LOCUS       DATBFZ010000001        10671 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_5578474, whole genome shotgun sequence.
ACCESSION   DATBFZ010000001 DATBFZ010000000
VERSION     DATBFZ010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 10671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10671
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5578474"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="VH419_00005"
     CDS             <1..470
                     /locus_tag="VH419_00005"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: protein motif:HMM:NF013962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEY7042028.1"
                     /translation="KGLRVGLDVYADEPASGRAQWSSKLAAHPNVVGTHHIGASTGQA
                     QRAVADGVVEVIDAFVEGQATNCVNLAPRLGVETLTVRHLDRVGVLARVLDLLSAERL
                     NVEQMQNRVFEGGEAAVATIDVGGEIDDGLLSRLRDIPDVLSVSLAFIQDSSD"
     gene            complement(499..1113)
                     /locus_tag="VH419_00010"
     CDS             complement(499..1113)
                     /locus_tag="VH419_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HEY7042029.1"
                     /translation="MTWHSSSHEVRDRVQTAFEIVAREPFLPRRQRRYAGEDRPLEIG
                     HGQTNSQPRTVRAMLEYLDPRPGDRVLDVGCGSGWTTALLGELVGPDGLVVGIELVPQ
                     LAKAARHNLTGAGEHIRVLAAEPDVLGAPDLAPFDKILVSAQARAMPDPLVQQLEIGG
                     RLVAPVRGVMTVVDRLGEDEVSIRRMGRYAFVPLVWQPPPDSSG"
     gene            1388..2314
                     /locus_tag="VH419_00015"
     CDS             1388..2314
                     /locus_tag="VH419_00015"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEY7042030.1"
                     /translation="MALSALPRLQPVAATHPAARRYSRARRNLLPRHDRGTAVSGRWA
                     HDLLLETGAEVETFLWCPGERQLSSEVVDHAAAVAETAASAYVVSERTLTRLHPGLSA
                     PALLSVVRLPTWRPQEVVTADAQLFLVADGIEYAGNLGTLVRTADACGADGLVLTNAD
                     ARITHPKVFVASRGTVLSLPVLEYADVRAARRDLSGAGFTVYVADPAAALSYREGAYA
                     FGRSAFVVGSEGDGVCAEWRTPDLTRVSIPMHGTADSLNVAASAAILLFEARGQLDRS
                     DSAQPHTRQDSQTIPSLSASTYQSYSGSRPRL"
     gene            complement(2239..3129)
                     /locus_tag="VH419_00020"
     CDS             complement(2239..3129)
                     /locus_tag="VH419_00020"
                     /inference="COORDINATES: protein motif:HMM:NF019151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excalibur calcium-binding domain-containing
                     protein"
                     /protein_id="HEY7042031.1"
                     /translation="MPRPLTALLVTAVALAGLAEPIGTASATSSSVIPVMQAKPLPRA
                     ASSKLSPPYAVNAKATIRIRFRAAVRSLHVARSTPRGYDRDKFGDWRDADGDCRDARA
                     EVLVAESRRPVSGGCTITRGRWFSSYDRRFWRRASDVDIDHVVPLAEVWRSGGKRWGT
                     QRRVAYANDLADPRTLRAVTDNVNQSKGDSDPAHWLPEFGRCSYVAQYTAVKIRWRLL
                     VDRREKQAMRHVAAHCPNVVIRVHRAKVVTRRHGNGLDPRFDTCAAAIAAGYGPYRRG
                     RDPEYDWYVDADNDGIVCES"
     gene            3170..3982
                     /locus_tag="VH419_00025"
     CDS             3170..3982
                     /locus_tag="VH419_00025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042032.1"
                     /translation="MSESVVPSADEHLTDGWEPELAADDTLLRQCVLVHASWPVAVAS
                     STGRPWHRDDRWAGAWIGDRGWFTNLACLLRPPDDPGSIVREIGEVIPAHVPYTLLNP
                     WPGDLSPYGLRLIGHPPLMVRFPGPAAPPLGVDVREVHDADHLAVAEQVLVEGYPMPE
                     LQPLSRGDLFGPAILGHATRVWLGFVDGEPVSVAAAHHDGGVTLVEYVAALPAARGRG
                     AGAAVTWAATLSEPTAPAVLIASDDGRPVYARMGYRAIERWHAWVRIPEPRD"
     gene            complement(4023..4358)
                     /locus_tag="VH419_00030"
     CDS             complement(4023..4358)
                     /locus_tag="VH419_00030"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HEY7042033.1"
                     /translation="MTSSAFSDVAVLLAAKQRQLSGQLAEMSAPPEEIGNISFGKRVG
                     EGTSQAVDRLSAVPAHDKLQQLLAEVVRAQAKLGDGTYGSCDVCGRDIGSDRLEARPW
                     ATRCIDHAQ"
     gene            complement(4355..5317)
                     /gene="ligD"
                     /locus_tag="VH419_00035"
     CDS             complement(4355..5317)
                     /gene="ligD"
                     /locus_tag="VH419_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009080793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEY7042034.1"
                     /translation="MEKISDVPGPALATLTKERFSRPNWIYERKLDGMRIIASRSGSR
                     VRIVSRRGADAGGSFPEIVAALQQIDAPDFVVDGEVVAFDGVQTSFAKLQPRMHVADP
                     AKARRSGVKVYYYLFDLLHLDGHSTRSLPLRERKALLKDLFDWDDPLRFTEHRNADGE
                     KYFAQACAKGWEGLIAKRADSPYRSGRTSEWLKFKCEQGQEFVVGGWTDPQGSRFGFG
                     ALLLGYYSADGRLVYAGKVGSGFDQKRLAAMTRQLQSITQDQPAYDLATLPSAPVFRR
                     GVHWVKPTLVAQVGFSEWTPDGQLRHPRFLGLRTDKAVREVVRE"
     gene            5483..6319
                     /locus_tag="VH419_00040"
     CDS             5483..6319
                     /locus_tag="VH419_00040"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY7042035.1"
                     /translation="MATFVIVHGGFGGAWEWTSVARLLRRLGHDVYTPTLSGLGERSH
                     LASAATIGLAAQVDDIVGLIEIEDLHDVVLCASDFGGVPATVAADRVSSRLSMLVYID
                     ALVPCDGEAAVDLLPTDFAMFVQEGLTEHGERWRVPAIVDPLPAPGWQTAAQMAAYRR
                     KLRDQPVMTFIEPAYLTGAVDFVQRVFVRCTMRADFADLLDVDPIRLVAWRAKMNGWQ
                     YDELAAGHDPHLTEPLATANHLAALAGSVPSHASAVPPELVRHLPKQRQSGLDSQNWA
                     WN"
     gene            complement(6899..8314)
                     /locus_tag="VH419_00045"
     CDS             complement(6899..8314)
                     /locus_tag="VH419_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY7042036.1"
                     /translation="MTIESTSAATAESLRGLCGGAVHLPGDPGYDGARVPWNVAVDQR
                     PAAVAYPANPREVSEVVVAAARAGLRVAPQTTGHNAAPLAAKGMDDVVLVRTSAMTNV
                     TVGAQSRIARAEGGALWLDAVEAATPHGLIPLHGSSPDVGIAGYSLGGGIGWYARKLG
                     LATNSITAVELVTADGAVVRADADTDPALFWALRGGGGNFGIVTALEFALYPIETAYA
                     GMLIWDRVHTEKVLRRWVDWTGQAPDEVTTSFRVLNLPPLPELPELLRGRQIVMIDGA
                     VLGSDSEAESILSGLRELSPEIDTFGRAPAGSLIRMHMDPEGPTPAVTHTSMLGALSD
                     EAIATFVSAVGPGSRSSLLLAELRQLGGALSRPHPGGGGALSHLEADFVVFAGAIAAT
                     PELAAQGYTDAVSLTTAMAPWATGRQYLNFAEDAVDPRAGYTSEAWEQLSAVRSMVDP
                     DGVFAANHTVPRLYEGGQPAV"
     gene            complement(8418..>10671)
                     /locus_tag="VH419_00050"
     CDS             complement(8418..>10671)
                     /locus_tag="VH419_00050"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEY7042037.1"
                     /translation="TISVAPAPLSRAGVADLVHERLGDAADAFVSACHTTTSGNPLLL
                     RQLLRALEADRVRPDALHADTVRAIGSRAVSSMVLRRFGRMPAENRAVARAVAVLGDG
                     AALPHVAALAGLSDERTALAVGSLARAEVLRDEYPLGFVHPLVRDAVYGDLSAGERQL
                     QHERAAAALIDSGATAEQVAAHLLLVPSRGDAKVVEVLREAADRDLHRGAPESSAAYL
                     HRALAEPPTPDVAAQILVDLGDVEARFDGRAAAGHLRQAYGALTDPAAKAEAGILLAR
                     TLVFVGQPGEATRFALAASAALPESLVDHRQGLVALARIAGFMHGLLPSEYAAGPRPE
                     PVGDGLGARALAATLAWETVLTGENRDQAIDYGGFALVGGELQRVDAGLLWVVAAMSR
                     EMGGVDTMPFWDQALADAHERGTLFAALATHLWRGYAQWRRGDLPEAYQSMLNCTDQN
                     RLYGAPEIGQPYTDAMTVWILLDRGEITKARGLVEEVRPRQRIGDGDRLFHEAEAQLL
                     IAEGHYDDALVCLDRADALTLTSRNPIWHRSPALRVEALRLLGRHEEALQLADEVVTA
                     ARRWGEPEVIGIALRLRGEIHSVDAETDLQDACDLLRDGRSRLEYARALAAYAGFQAR
                     SRPGQPPDAAAMATLQEAFELAEQCRAFGLRRDLVDDLARGGVAVPAEPRLAQQFTTT
                     EQRIVRMAVDGLGNREIAQALFMTPRTVELTLTSVCERLGAATIAEVTSSSLPPDLSS
                     PLSVREPVAD"
BASE COUNT         1542 a         3717 c         3782 g         1630 t
ORIGIN      
        1 agaagggtct gcgggtcggg ctcgacgtgt acgccgacga gcccgcgagc ggccgagcgc
       61 aatggagctc caagctggcc gcacacccca acgtcgtcgg gacgcaccac atcggcgcgt
      121 cgaccgggca ggcgcagcgt gcggtcgcgg acggggtcgt cgaggtcatc gacgccttcg
      181 tcgagggtca agccaccaac tgcgtcaacc tggcgccgcg gctcggggtc gagacactca
      241 ccgtccggca cctggaccgc gtcggcgtgc tcgcccgggt cctcgaccta ctgagcgcgg
      301 aacgcctcaa cgtcgagcag atgcagaacc gcgtcttcga gggcggggag gcggcggtcg
      361 cgaccatcga cgtcggtggg gagatcgacg acgggctgct gagccgcctg cgcgacatcc
      421 cggacgtcct gagtgtctcg ctcgcgttca tccaggattc gtccgactga tcccggccga
      481 gatcgtcagc ggtcactctc agcccgacga gtcgggaggt ggctgccaga ccagcggcac
      541 gaacgcgtag cggcccatcc ggcgtatcga cacctcgtcc tcgccgagcc tgtccacgac
      601 ggtcatcacg ccgcgaaccg gtgccaccag ccggccaccg atctcgagct gctggaccag
      661 cgggtcgggc atggcgcgcg cctgcgccga caccaggatc ttgtcgaacg gggcgaggtc
      721 cggggcaccg aggacgtccg gttccgcggc cagcacgcgg atgtgctcac cggcgccggt
      781 gagattgtgt cgggccgcct tggcgagctg ggggacgagc tcgatcccga ccacgaggcc
      841 gtccgggcca accagctcac cgagcagcgc ggtcgtccaa cccgatccgc agccgacgtc
      901 gagcacgcgg tccccagggc gcggatcgag gtactccagc atcgcgcgca ccgtccgcgg
      961 ctgggagttg gtctgaccgt ggccgatctc cagcgggcgg tcctcgccgg cgtagcggcg
     1021 ctgccgacgt ggcaggaacg gctctcgggc gacgatctcg aaggccgtct gcacccggtc
     1081 gcggacctcg tgcgaggagc tgtgccaggt catgggtcgt gagcggcgcc ggtcgtgtgg
     1141 ctggtcgtcc ggctggacgt ggtacgcctg tgacgcctgg tgtaccaaac tatcgcaggt
     1201 gcggtggctt gaagaaccaa ccgcaccatc gctaaaacca ggtgcgggag cgtctgtcgg
     1261 tgtctaccct cgggcgcatg ggtagaacgg agtgacttcc accaggcgcc gttcggctgc
     1321 ctgacgacca gcgcgcgcga cccgctcggc gtgccggtca ggtcgactca cttctccgga
     1381 gcactccatg gccctgtccg cgttgccgcg actccaaccc gtcgccgcca ctcaccccgc
     1441 cgcccgccgc tactcgcgtg cgcggcgcaa cctgctgccg cgccacgacc gaggcactgc
     1501 ggtctccggc cgctgggccc acgacctgct gctcgagacc ggtgccgagg tcgagacgtt
     1561 cctgtggtgt ccgggcgagc gccaactctc gtcggaggtt gtggaccacg ccgcagcggt
     1621 cgccgagacg gcggcttcgg cgtacgtcgt ctctgaacgc accctcacgc ggctgcaccc
     1681 cggcctcagc gcacccgccc tgctgtccgt cgtgcggcta ccgacctggc ggccgcagga
     1741 ggtcgtcaca gccgacgcgc agctgttcct cgtggccgac ggcatcgagt acgccggcaa
     1801 cctcgggacg ctcgtccgga ccgcggacgc gtgtggtgcg gacggactgg tgctgacgaa
     1861 cgccgacgcc agaatcacgc atcccaaggt gttcgtggca agccggggga ccgtgctctc
     1921 gttgccggtg ctcgagtacg ccgacgtgcg tgctgcacgg cgggacctgt cgggcgccgg
     1981 cttcacggtg tacgtcgccg atccagccgc cgctctgtcg tatcgggagg gtgcgtacgc
     2041 gttcggacgg tccgcgttcg tggtcgggtc cgagggcgat ggtgtctgcg ccgagtggcg
     2101 tacacccgac ctgacccgag tctcaatccc gatgcacggg acggcggact cgctcaacgt
     2161 cgcggcgtca gcggcgatcc tgctgttcga ggcgcgcggc cagctcgacc ggtcggacag
     2221 cgcgcagcct cacacccgtc aggactcgca gacgatcccg tcgttgtcgg cgtcgacgta
     2281 ccagtcgtac tccgggtcgc gaccgcgtct gtaggggccg tagccggcgg cgatcgccgc
     2341 ggcgcaggtg tcgaaccgtg ggtcgaggcc gttgccgtgc cggcgggtga cgaccttggc
     2401 ccggtgcact ctgatgacga cgttcgggca gtgcgcggcg acgtgccgca tcgcctgctt
     2461 ctcacggcgg tcgacgagca gtcgccagcg gatcttcacc gcggtgtact gggcgacgta
     2521 cgagcaacgg ccgaactccg ggagccagtg cgcgggatcg ctgtcgccct tgctctggtt
     2581 gacgttgtcg gtgaccgccc gcagcgtgcg cgggtcggcg aggtcgttcg cgtaggccac
     2641 gcgccgctgc gtgccccatc gcttgccgcc ggaccgccac acctcggcga gcgggaccac
     2701 gtggtcgatg tcgacatcgc tcgctcgccg ccagaaccgg cggtcgtagg aggagaacca
     2761 tcgcccccgc gtgatcgtgc agccacctga cacaggcctg cgcgactcgg cgaccagcac
     2821 ctcggcgcgg gcgtcgcggc agtccccgtc ggcatcccgc cagtcgccga acttgtcccg
     2881 gtcgtaccca cgaggcgtcg atctcgcgac gtgcaacgac cggaccgcgg cccggaacct
     2941 gatacggatc gtcgccttgg cgttgacggc gtacggcggc gacagcttcg acgacgccgc
     3001 tctcggcagc ggtttcgcct gcatgacggg aatcaccgaa gacgacgtcg cactggccgt
     3061 cccgatcggc tcggcgaggc cggcgagggc gactgccgtg acgagcagag cggtgagggg
     3121 tcgtggcacg cgttgacggt acctgggcct tcggctgtat aggtggaaga tgagcgaatc
     3181 ggtcgtgccc tccgccgacg aacatctcac cgatggttgg gagcctgagc tcgccgccga
     3241 cgacacgctg ctccggcagt gcgtgctcgt gcatgcgtcg tggccggtcg ccgtggccag
     3301 cagcacaggt cgaccgtggc accgcgacga ccgctgggcg ggcgcgtgga tcggcgaccg
     3361 gggctggttc acgaaccttg cgtgcctgct gcgtcccccg gacgatccag ggtcgatcgt
     3421 ccgcgagatc ggtgaggtca tccctgcaca cgtgccgtac acgctgctga acccgtggcc
     3481 cggcgacctg tcgccgtacg gactgagact gatcgggcat ccgccgctga tggtccggtt
     3541 cccaggtccg gcggccccgc cgctcggtgt cgatgtgcgg gaggtccacg atgccgacca
     3601 cctcgcagtg gcggagcagg tcctcgtcga gggctatccg atgccggagc tccagccgct
     3661 gtcgcgaggc gatctgttcg ggcccgccat cctcgggcac gcgacgcggg tgtggctcgg
     3721 cttcgtcgac ggtgagccgg tgtcagttgc ggctgcgcac cacgatggtg gagtgacgct
     3781 ggtggagtac gtcgccgcgc tgccggccgc tcgtggccgc ggcgccggtg ccgccgtgac
     3841 ctgggcggcg acgctgtcgg agccgaccgc gccggccgtg ctcatcgcca gtgacgacgg
     3901 gcgacctgtg tacgcgcgga tgggctaccg tgcgatcgag cgctggcacg cctgggtgag
     3961 aatccccgag ccccgggatt gagcaggtcc gagcgccatc caccggatcc gttggctgcg
     4021 tgtcactgtg cgtggtcgat gcaccgagtc gcccacggcc tggcctcgag ccggtcggag
     4081 ccgatgtccc ggccacagac gtcgcaactt ccgtaggtcc cgtcgccgag cttggcctgc
     4141 gctcgcacga cctcggcgag cagctgctgg agcttgtcgt gcgcggggac ggcggacagc
     4201 cggtccaccg cctggctggt gccttcaccg acgcgcttgc cgaacgagat gttgccgatc
     4261 tcctcagggg gtgcgctcat ctcggccagc tggcctgaga gctgccgctg cttggccgcg
     4321 agcaggacgg cgacgtcgga gaatgcggag ctcgtcattc acgcaccacc tcgcggacgg
     4381 ccttgtcggt gcgcaggcca aggaagcggg ggtgacgcag ctgaccgtcc ggcgtccatt
     4441 cggagaagcc gacctgtgcg accaacgtcg gcttcaccca gtggacgcca cgtcggaaga
     4501 cgggggcgct cggcagcgtg gccaggtcgt atgccggttg gtcctgcgtg atcgactgga
     4561 gctgcctcgt catcgccgcc agacgcttct ggtcgaagcc gctcccgacc ttgccggcgt
     4621 acacgagtct gccgtccgct gagtagtagc cgagcaacag cgcgccgaag ccgaaccgcg
     4681 agccctgcgg gtcggtccag ccgccgacga cgaactcctg gccctgctcg cacttgaact
     4741 tcaaccactc ggacgtgcgt ccggatcggt agggcgagtc ggctcgtttc gcgatcaggc
     4801 cctcccagcc cttggcgcag gcctgcgcga agtacttctc gccgtcggcg ttgcggtgct
     4861 cggtgaagcg gagcgggtcg tcccagtcga acaggtcctt gagcagtgcc ttgcgctcgc
     4921 gcagcggaag cgaccgggtg ctgtggccgt cgaggtggag caggtcgaag aggtagtagt
     4981 agaccttcac cccgctccgc cgtgcctttg ccgggtcggc cacgtgcatc cggggctgga
     5041 gcttcgcgaa gctcgtctgg acgccgtcga acgccaccac ctcgccgtcg accacgaagt
     5101 cgggtgcgtc gatctgttgg agagccgcca cgatctccgg gaagctgccg ccggcatcgg
     5161 cgcctcgccg gctgacgatc cggacccggc tgccggagcg ggaggcgatg atgcgcatgc
     5221 cgtcgagctt gcgctcgtag atccagttcg gacgggagaa gcgttctttc gtcagcgtgg
     5281 ccagcgccgg gccggggacg tcggagatct tctccatgtc cgcagggtag tgctggagac
     5341 tcccagtgag gcgagtacga cgtacggtga caccagtgcc gctcgggacc acacactgcc
     5401 catatgaccg ctgatcctgg tgatggcggg tcgcccgttc ggactattgc cgccggcgtg
     5461 atcggcgtcg ttccattcga ctatggccac tttcgtcatc gtgcacgggg gattcggcgg
     5521 tgcctgggag tggacctcgg tggcgcggct cctacgtcgg ctcggtcacg acgtctacac
     5581 gccgaccctc agcggcctcg gcgaacgcag ccaccttgct tcggccgcca cgatcggtct
     5641 ggcggcgcag gtcgacgaca tcgtcgggct gatcgagatc gaggacctgc acgacgtcgt
     5701 cttgtgtgcg agtgacttcg gcggcgtacc ggcgacagtc gcggccgacc gggtgtcgtc
     5761 gagactgagc atgctcgtgt acatcgatgc actcgtgccc tgcgacggcg aggcggcggt
     5821 cgacctgctg cccaccgact tcgcgatgtt cgtccaggag gggctgaccg agcatggtga
     5881 gcgctggcgg gtgcctgcca tcgtcgaccc gctcccggcg cccggttggc agacggcggc
     5941 gcagatggcg gcataccgac gaaagctgcg cgaccagccg gtgatgacgt tcatcgaacc
     6001 ggcgtacctc accggggccg tcgacttcgt gcaacgggtc ttcgtccggt gcacgatgcg
     6061 ggccgacttc gcggacctcc tcgacgtcga cccgatccgg ttggtggcct ggcgagccaa
     6121 gatgaacggg tggcagtacg acgagctggc cgccggacat gatccgcacc tcaccgagcc
     6181 gctggcgacc gccaaccacc tggcagcact ggcgggctcg gtcccatcgc acgccagtgc
     6241 agttccgccg gagctggtgc ggcatctgcc gaagcagcgt cagtccgggc tcgacagcca
     6301 gaactgggcc tggaactgac cctggaactc accctggaac tgacacagtg acgtgcaccg
     6361 actgccgggc ttccgccgat tgcggatgga cgcgtcgcag cggcgaagcc gcgcggcgcg
     6421 gctcaatatg ccacctgcca acgctggctg tcggcaatcg cgggaagcca ctgtccacag
     6481 ggggcgatca gaaactgtgg agaagtggat gtattccgtg gacgacgcgg ccgcttctgt
     6541 gggtaactcg ccaggccgga aaattccttg aacttctttg cagcaaaggg ttgtgcgacc
     6601 gcttcgggcc cgtgtagaaa tctctcacgc cttccgagag ggaggcggga tcggaaggga
     6661 aaccgaggat ccatccggcg gggcgacctg ccgcggcagc cggtgacgag gctgtcggga
     6721 tctgacggac cgaccggtcg gacggaggcg tcacatccct ccgaccaccc gtagggcctc
     6781 tcaacctcat acgttgagag gccctacacc tgtctgtgga ccgtcatcgg acctcggcgg
     6841 ccgggcttgt cgagcccccc gtctcgacaa gcccggtcag cgcgtcccgt ccggctcgtc
     6901 agacggccgg ctgcccacct tcgtacaagc ggggcaccgt gtggttggcc gcgaagaccc
     6961 cgtccgggtc gaccatcgag cgcaccgccg acagctgctc ccacgcctcg ctggtgtagc
     7021 ccgcccgcgg gtcgacggcg tcctcggcga agttcaggta ctgccgaccc gtcgcccacg
     7081 gcgccatcgc cgtcgtcagg ctgaccgcgt ccgtgtaccc ctgtgccgcc agctcaggcg
     7141 tcgccgcgat ggcgcccgcg aacactacga agtcagcctc caagtgggaa agagcgccgc
     7201 cgcccccggg gtgcgggcga gacagcgcgc cgccgagctg ccggagctcg gccagcagca
     7261 gtgatgaccg cgagccgggt cccacggccg agacgaacgt cgcgatcgcc tcgtcggaga
     7321 gcgcgccgag catcgatgtg tgcgtcacgg ccggcgtcgg tccctctggg tccatgtgca
     7381 tccggatcag cgatccggcc ggggcccgtc cgaacgtgtc gatctcaggg gacagctcgc
     7441 gcaggccgga aaggatcgac tccgcctcgc tgtcgctgcc gagcacggcg ccgtcgatca
     7501 tgacgatctg gcggccgcgg agcagctccg gcagctccgg cagcgggggg aggttgagga
     7561 cgcggaacga cgtggtgacc tcgtccggag cctgtccggt ccagtccacc cagcggcgca
     7621 gcaccttctc ggtgtgcacc ctgtcccaga tgagcatgcc ggcgtacgca gtctcgatcg
     7681 ggtacagcgc gaactccagc gcggtcacga tgccgaagtt gccgccgcca ccgcgcagcg
     7741 cccagaagag cgcgggatcg gtgtcggcgt cagcccggac gacggcgccg tcagcggtca
     7801 cgagctccac ggccgtgatg ctgttcgtgg cgaggccgag cttgcgcgcg taccagccga
     7861 taccgccgcc gagggagtag ccggcgatgc cgacgtccgg ggacgagcca tgtagcggga
     7921 tgagtccgtg cggcgtggcg gcctcgaccg cgtcgagcca cagggctccg ccttcggctc
     7981 gtgcgatgcg ggactgggcg ccgacggtga cattggtcat cgccgaggtg cggacgagta
     8041 cgacgtcgtc cattcccttc gccgccagcg gggcagcgtt gtggccagtg gtctgcggag
     8101 ccacccgaag tccggcgcgg gcggccgcga cgacgacctc ggacacctct cgtgggttgg
     8161 ccgggtaggc aacggctgcg gggcgttggt cgacggccac gttccaggga acacgcgccc
     8221 cgtcgtatcc agggtcgccc ggcaggtgga ccgcgccgcc gcacaggccg cgcaagcttt
     8281 ccgcggtggc ggcggaggtg gactcgatgg tcatgtgagc tccttggtca gctactcgtt
     8341 gaggtctgac caggagcatg cgggcgaccg gttgggagac acttgcggtg cacttgagcc
     8401 ccgccgagac gggacggtca gtcggcgacg ggctcgcgga cgctgagcgg cgaggacagg
     8461 tccggcggga gtgagctcga ggtgacctcg gcgatcgtgg ccgcgcccag gcgctcacag
     8521 accgatgtca acgtcagttc caccgtccgc ggcgtcatga agagcgcctg cgcgatctcc
     8581 cggtttccga gcccgtcgac cgccatccgg acgatccgct gctcggtggt ggtgaactgc
     8641 tgcgccagcc tcggctccgc aggcacggcg acgccgccgc gcgcgaggtc gtccaccaga
     8701 tcccggcgca gcccgaaggc acggcattgc tcggccagct cgaacgcctc ctgcagcgtc
     8761 gccatcgccg ctgcgtccgg gggctggccg ggtctggacc gggcctggaa gccggcgtac
     8821 gccgcgagcg cccgggcgta ctcgagccgg gacctaccgt cgcggagcag gtcgcacgcg
     8881 tcctggaggt ccgtctcggc gtcgacgctg tggatctccc cgcggagtct cagcgcgatg
     8941 ccgatgacct ccggctcgcc ccaccgccgg gccgctgtca cgacctcgtc cgcgagctgg
     9001 agcgcctcct cgtggcggcc caacaggcgc agcgcctcga cccggagcgc cggggagcga
     9061 tgccagatcg ggttcctgct cgtgagcgtc agcgcgtcgg cacggtcgag acagaccagc
     9121 gcatcgtcgt agtggccctc cgcgatgagc agttgagcct ccgcctcgtg gaagagccga
     9181 tcgccgtcgc cgatccgctg acgcggccgg acctcctcga ccagcccgcg cgctttcgtg
     9241 atctcacccc ggtcgaggag gatccagacg gtcatcgcat cggtgtaggg ctgtccgatc
     9301 tccggggcgc cgtacagccg gttctggtcc gtgcagttca gcatcgactg gtacgcctcg
     9361 ggcaggtcgc cgcgacgcca ctgtgcgtac cctcgccaaa gatgggtcgc gagggcggcg
     9421 aacagcgtgc cgcgttcgtg tgcgtctgcc aacgcctggt cccagaacgg catcgtgtcg
     9481 acgccgccca tctcccgtga catcgcggcg acgacccaga gcagacccgc gtcgacgcgc
     9541 tgcagctctc cgccgaccag ggcgaagccg ccgtagtcga tcgcctggtc gcggttctcg
     9601 ccggtgagga cggtctccca ggcgagggtc gcggcgagcg cacgagcgcc gagcccgtcg
     9661 ccgaccggct ccggacgtgg tcccgcggcg tactccgacg ggagcagtcc gtgcatgaac
     9721 cccgcgatcc gcgccagcgc caccaggccc tggcgatggt cgaccaagct ctcggggagc
     9781 gcggcgctgg ccgcgagggc gaaccgtgtc gcctcgcccg gctgcccgac gaacacgagg
     9841 gttcgcgcga gcaggatgcc ggcctcggcc ttcgccgccg ggtcggtcaa cgcgccgtat
     9901 gcctggcgca ggtgtccggc cgccgcccgg ccgtcgaagc gtgcctcgac gtcgccgagg
     9961 tcgacgagta tctgcgccgc cacgtccggg gtcggtggct cggcgagtgc tcggtgcagg
    10021 tacgccgccg agctctccgg cgcaccgcga tgcaggtcgc gatcggccgc ctcccggagc
    10081 acctcgacca ccttcgcgtc gccacggctc ggcaccagga gcaggtgggc agcgacctgc
    10141 tcggccgtcg ccccggagtc gatcagcgca gcggccgctc gctcgtgctg cagctggcgt
    10201 tccccggcgg acaggtcgcc gtacaccgcg tctcgaacca gcggatgcac gaacccgagc
    10261 gggtactcgt cacgaaggac ctcggcccgt gcgagcgacc cgaccgcgag tgcggtgcgt
    10321 tcgtccgaca gacccgccaa ggcggcgacg tgcggcaacg cggcgccgtc cccgagcacc
    10381 gccacggcac gggccacggc ccggttctct gcaggcatcc ggccgaaccg gcgaagcacc
    10441 atgctcgaga ccgcccgcga cccgatcgcc cggaccgtgt ccgcgtggag cgcgtcggga
    10501 cggacgcggt ccgcttcgag tgcccgcagc aattgccgca gcagcagcgg gttgcccgac
    10561 gtggtggtgt ggcaggcgga gacgaaggcg tcggccgcgt caccgagccg ttcgtgaacc
    10621 aggtcggcca cccccgctcg cgagagtggc gccggtgcca ccgagatcgt c
//
LOCUS       DATBFZ010000002         3463 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_986199, whole genome shotgun sequence.
ACCESSION   DATBFZ010000002 DATBFZ010000000
VERSION     DATBFZ010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 3463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3463
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_986199"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            267..3353
                     /locus_tag="VH419_00055"
     CDS             267..3353
                     /locus_tag="VH419_00055"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY7042038.1"
                     /translation="MADDTDGPVPFSPTELRTFLIADVRGYTRFTKEQGDEAASDLAA
                     SFAEIVRSTVPEFAGELLELRGDEALCVFGSARQALRAAVDLQRRLRTPSDEDGSVFP
                     LGVGVGLDAGEAVPTEGGYRGGALNLAARLCSVAAPGQVLASETVVHLSQRVDGVQFS
                     TARTRRFKGIETPVRLVEVIPETPLPAVPVVPAPKRRRSTAWMVAAVALAVAAVSAIG
                     AIYMRGNDGSSVGLASNSFSLVSSDGHASTPLTIDEAPFGVAVDGKVVWLPRYSDDAV
                     LSLDTVTDEKELVQVGDGPDAVAVGEGSVWVANSGDGTVSEVSPAAGRTVGDPIHVGN
                     GPSGIAVGEGAVWVALSVDGAVAKIDPDTGKVVDTFSAGTNPTRVAVDFGKVWVTNES
                     VGTVTPIDPATDTAGTPIAVGHGPNGITTGADAVWVTNSLDGSVSRIDPDTLVVATFP
                     TRGDDTQGIAVVGDTVWVAARRSAKIVRLDATTGQIRSNLAVGANPQDVATLGDGAAI
                     TTTTSPREHRGGTLTIAGASARALRDRPSVDPDSFAAWYPVVWDSLAVTNDGLVSLKR
                     VPGPDGGTVVADLATALPTVTDGGLTYAFELREGIRYSTGRPVHASDIRYGLERTFTV
                     NAGYYLQVRPPLDHEPYGAIVGAPKCLASPRSCDLSRGIVVDDKTGRITFHLRHPDPD
                     LIAKLAMPLAVAIPPDAPRHDSGRHPLPATGPYMVARYGPIRQYILVRNPYFHEWSAD
                     AQPDGYPERIFWQIYKTTSEAVSAVEHGVADWLYFTIPGINPQQTREIEADYAAQIHP
                     SAYPSTDFLLFEPNSPLSRDRAARHAIAYAIDRDRLRTILKSGSSSLPAPPTCQLVPI
                     NFLGYRPYCPYKLDPARAQKLVHRSPNYGKPVSVVSYVGGTGADRYVVELLNSLGFRA
                     RLAPLDEDGNPKEPQDITLYSWAADYVGPADFILGIRTGLITPGDLTDAYAKQSESQF
                     QGTRAWAAADRHLTDNALVIPIGTGSTLGFTSKRVGNYQTAPAPGNSPMIDQMWVH"
BASE COUNT          586 a         1165 c         1158 g          554 t
ORIGIN      
        1 gcggcgccgg ccgcccctcc cagtcgacgc tcggcccgtg ccagcacgag aactggatgt
       61 cggcgatcag gcggtcgagt gccttgatgt ctgcgctatt catgtgattc agcccccttg
      121 tgaatcgttg ccctcgatct acataacggc agacctcggt tccggatcaa ggctgggacg
      181 aaaaccttga cagaccgcga acaattggcg gctcgccggg ctgcgaaaca cgcaggtcgt
      241 tgcgtttgtc gtagactccg gcgatcgtgg cagacgatac ggacgggccg gttccgttct
      301 cgccaacgga gctgcggaca ttcctgatcg cggatgtccg cggatacacg cggttcacca
      361 aggagcaggg tgatgaggcc gcttcggatc tcgcggcgtc cttcgccgag atcgtccgca
      421 gcacggtccc ggagttcgcg ggcgagctcc tggagctgcg cggcgatgag gcgctgtgcg
      481 tgttcgggtc ggcgcggcag gcgctgcggg cggcggtgga cctgcagcgg cggttgcgca
      541 cgccgtcgga cgaggacggc agcgtcttcc cgctgggggt gggtgtgggg ctggatgccg
      601 gcgaggcggt gccgaccgag ggtggctatc gtggcggtgc gctgaacctg gccgcgaggc
      661 tctgctcggt cgccgcgccg ggccaggttc tggccagcga aaccgttgtc catctctcgc
      721 aacgcgtcga cggagtccag ttctcgaccg cgcgaacgcg ccggttcaag gggatcgaga
      781 cgcccgtgcg gttggtggag gtcatcccgg agaccccgct ccccgccgtt ccggtcgtgc
      841 ccgcaccgaa gaggcggcgg tcgacagcat ggatggtcgc ggccgtggcg cttgccgtgg
      901 ccgcggtgtc cgcgatcggt gccatctaca tgaggggaaa cgacgggtca tcggtcgggc
      961 tggcgagcaa ctcgttcagc ctggtctctt cggacgggca cgcgagcacg ccgctcacga
     1021 tcgatgaggc gccgttcggg gtggccgtcg acgggaaggt cgtatggctg ccgcgctact
     1081 cggacgacgc cgtcctgtcc ctggacacgg tcacggacga gaaggagctg gttcaggttg
     1141 gcgacgggcc ggatgcggtc gccgtgggtg agggcagcgt gtgggtggcc aactcgggcg
     1201 acggcaccgt gtcggaggtg agcccggcgg ctggcaggac ggtcggtgac ccgatccatg
     1261 tggggaacgg cccgtcggga atcgcggtcg gggagggagc cgtgtgggtg gcgctcagcg
     1321 tcgacggcgc ggtcgcgaag atcgacccag acactggcaa ggtcgtcgac accttctcgg
     1381 ccgggacaaa cccaacgcga gtcgcagtcg attttggcaa ggtgtgggtc accaacgagt
     1441 cggtcgggac ggtgacaccg atcgatccgg cgaccgatac cgcgggtacg ccgatcgcag
     1501 tcggccacgg acccaacggc atcaccaccg gagccgacgc ggtctgggtc accaacagcc
     1561 tcgacgggtc ggtctcgcgg atcgacccag acacactcgt ggtggccacg ttcccgaccc
     1621 gaggcgacga cacgcagggc atagcggtgg tcggcgacac cgtctgggtg gcggcccgcc
     1681 ggtccgcgaa gatcgtccgc ctcgatgcca cgacgggcca gatcagatcg aacctggcgg
     1741 tgggtgccaa tccgcaggat gtcgcaaccc tcggcgacgg cgcggcgatc accaccacga
     1801 catccccgag ggagcaccgc ggcgggacat tgaccatcgc gggcgcaagc gcgcgcgcgc
     1861 tccgtgaccg tccatcggtc gaccccgaca gctttgccgc gtggtacccc gtggtgtggg
     1921 attcgcttgc ggtgacgaac gacggccttg tcagcctgaa acgagtaccc ggcccggacg
     1981 gcgggacagt ggtcgcggat cttgcaacgg cgcttccgac ggtcaccgac ggcggcctca
     2041 cgtacgcgtt cgagctccgc gaggggatcc gatactcgac tggtagaccg gtccatgcga
     2101 gcgacatccg gtacggcctt gagcgcacgt tcacggtcaa tgccgggtac tacctccagg
     2161 ttcggccacc tttggaccac gagccgtacg gcgcaatcgt cggcgcgccg aaatgcctgg
     2221 cgtccccgag gtcgtgcgac ctcagccgcg ggatcgtggt ggacgacaag accgggagga
     2281 tcacgtttca cctgcgacat cccgatcccg acctcatcgc gaaactcgcc atgccacttg
     2341 cggttgcgat tcccccggat gcccctcggc acgactccgg tcgccacccg ttgcctgcca
     2401 ccggcccgta tatggtcgcc cgctacggac ccattcgaca gtacatcctc gtacgcaacc
     2461 cgtacttcca cgaatggtca gcggacgcgc aaccggacgg ataccccgaa cgcatcttct
     2521 ggcagatcta caagacgacc agcgaggcgg tttccgcggt cgagcacggc gtcgccgact
     2581 ggctctactt cacgatccca ggcatcaacc cgcagcagac tcgcgagatc gaagccgact
     2641 acgccgccca gatccatcct tcagcgtacc cctccaccga cttcctgctc tttgagccga
     2701 actcgccgtt gagtcgcgac cgcgcagcgc gacacgcgat cgcatacgcc atcgaccgcg
     2761 accggctccg cacgatcctg aagagcggca gcagcagcct cccggcgcca ccgacgtgcc
     2821 agctggtccc catcaacttc ctcgggtacc ggccctactg cccatacaag ctcgaccctg
     2881 cccgtgctca gaagctcgta caccgctcgc caaactatgg caagccggtc tcggtcgtct
     2941 cctacgttgg ggggacgggc gccgaccggt atgtcgtcga gcttctgaac agccttgggt
     3001 tccgcgcgcg gctcgccccg ctggatgaag acgggaatcc aaaggaaccg caagacatca
     3061 ccctctactc ctgggccgct gactatgtgg gtcccgcgga cttcatcctc gggattcgga
     3121 cggggctcat caccccgggt gacctcaccg atgcctacgc aaaacagagc gagagtcagt
     3181 tccaaggcac gcgcgcctgg gcggccgccg accgacacct cacggacaac gcgctggtca
     3241 tcccgatcgg caccggcagc accctcgggt tcacatccaa acgagtcggc aactaccaaa
     3301 ccgcgcctgc gccggggaac tccccgatga tcgaccagat gtgggtccac taacgtcccg
     3361 tcagagcgtc ttgaactgct cgaacggctg cttgcacgca tcgcagtagc ggatcgagcg
     3421 gcacggcgtc ggcccgaaca ggttctcgag gtgcgtgtcg gac
//
LOCUS       DATBFZ010000003         1848 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_3283449, whole genome shotgun sequence.
ACCESSION   DATBFZ010000003 DATBFZ010000000
VERSION     DATBFZ010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 1848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1848
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3283449"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..301)
                     /locus_tag="VH419_00060"
     CDS             complement(50..301)
                     /locus_tag="VH419_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008357797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY7042039.1"
                     /translation="MSVTTPHHAPFHERHDDELLTLSEVAEILSASENTVRWWRQIGS
                     GPSFFKIGRRLYTTVGELRRFIREQRLASRPVISATRRN"
     gene            481..1017
                     /locus_tag="VH419_00065"
     CDS             481..1017
                     /locus_tag="VH419_00065"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY7042040.1"
                     /translation="MRRLISRARRGSGLTQRQLAQRARTSQATLSAYERGLKCPNLAV
                     AERIVEAAGYRLDLVTHVHFEHHIARGVRPFWVPDRLWRGKLPECFAKVYLDDPATFE
                     GIRKFDLRRRPQRRRLYELLLRKGQPDELMDWIDGALLVDLWEDLTIPAVIREAWQPA
                     VTVAAGGPRERPWYGAMA"
     gene            complement(1007..1405)
                     /locus_tag="VH419_00070"
     CDS             complement(1007..1405)
                     /locus_tag="VH419_00070"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEY7042041.1"
                     /translation="MRIVLDTDVASGLIKGTLSPAMLAAIAPHEAALSFITVGELARW
                     IHARDLGQRRRRAVEAFFAAHPVIPGGRDVGAKWGEIVAYAVKRGRPRPFNDSWIAAS
                     CLAYDLSLATLNVTDFEDFAEYEGLRLITP"
     gene            complement(1409..1603)
                     /locus_tag="VH419_00075"
     CDS             complement(1409..1603)
                     /locus_tag="VH419_00075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042042.1"
                     /translation="MTSDPRSEPPPEWVSDKEDLDALFKDAKPIGDGSDWACQDFFES
                     DEEQAEFVAWYRAERHKELA"
BASE COUNT          294 a          584 c          642 g          328 t
ORIGIN      
        1 gtgcccgact cgtgggtcca ggtgagtatc cgcctgcgga tgctggccat cagttcctcc
       61 tcgtggccga gatgaccggt cgggaggcga gtcgctgctc ccggatgaag cggcggagct
      121 cgccgaccgt ggtgtagagc cgtcgtccga tcttgaagaa cgacgggccg ctgccgatct
      181 ggcgccacca gcgcaccgtg ttctcggagg ctgacagaat ctcagcaact tccgacaggg
      241 tgagcagctc gtcgtcgtgg cgctcgtgga agggggcgtg gtggggggtg gtcacggaca
      301 tggttgcctc cgagtgatgg gggttgttgg ggttgttcgt ctacttcaag cgcggcgccc
      361 tccggcgaat ggacacctgg ggagaaccat cggtgggacc gcgttcacca ggtaggtgcg
      421 cgcgaggcca ctcttcaatc ggcgtgtcga cgtccgcccg gatatcgtgg gcgcatagaa
      481 gtgaggcgac tgatctctcg ggctcggcga gggagcggcc tgacccagcg ccagctcgca
      541 cagcgggcga ggacgtccca ggccaccttg tcggcctacg agcgcggctt gaagtgtccg
      601 aacctagcgg tcgccgaacg gatcgtggag gccgcaggct accggctcga cctggtcacc
      661 cacgtccact tcgagcacca catcgctcgc ggggtgcgac cgttctgggt gcccgaccgt
      721 ttgtggagag gcaagttgcc ggagtgcttc gccaaggtct acctcgatga tccggcgacg
      781 ttcgagggga tccggaagtt cgacctacgc cgtcggcccc aacggaggcg gctgtacgaa
      841 ctcctgcttc gcaaggggca acccgacgag ctgatggact ggattgacgg cgctctgctc
      901 gtcgatctct gggaagacct cacgattccg gcggtcatcc gggaggcatg gcagccggct
      961 gtcacggtcg ccgccggtgg tccgcgcgaa cgcccctggt acggcgctat ggcgtgatca
     1021 ggcgaaggcc ctcgtactcc gcgaagtctt cgaagtcggt gacattgagg gtcgccagcg
     1081 agaggtcgta ggcgaggcag ctggctgcga tccacgaatc gttgaacgga cgtgggcggc
     1141 cccgcttgac ggcgtaggcg acgatctcgc cccacttcgc ccccacgtca cgaccacccg
     1201 gaatgaccgg gtgggccgcg aagaacgcct cgacggcccg acggcgccgc tgaccgaggt
     1261 ctcgggcgtg aatccatcga gccagctcgc cgaccgtgat gaatgacaga gctgcttcat
     1321 gcggcgcgat ggcagcgagc atcgcaggcg acaacgtccc tttgatcaga cccgacgcca
     1381 cgtcggtgtc gagcacgatc ctcacggctc aggcaagttc cttatggcgc tcggcccggt
     1441 accacgcgac gaactccgcc tgttcctcgt cggactcgaa gaaatcctgg catgcccagt
     1501 cggagccgtc accaatcggc tttgcgtcct tgaagagcgc gtcgaggtct tccttgtcgc
     1561 tgacccactc gggtggcggc tcagaccgcg ggtcgctggt catggcagcc tccttggcga
     1621 tcatcgaccg atggctcaag tgtggcacgt cgcactgacc ttccctcgga cgctcgtcag
     1681 agaccgatgc tcggaccttg cgcctgcgac ggtggtcggt agccgaagtc gtcttcgacg
     1741 cgacgggccg gagtcggagg ccgtcgggct ggccgcttgg ctttcggccc gtagcggtca
     1801 acggcctctg cggcaagtcg tgcggcgtgg gccggtccga ggtcggct
//
LOCUS       DATBFZ010000004         3700 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_1642863, whole genome shotgun sequence.
ACCESSION   DATBFZ010000004 DATBFZ010000000
VERSION     DATBFZ010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 3700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3700
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1642863"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..744
                     /locus_tag="VH419_00080"
     CDS             <1..744
                     /locus_tag="VH419_00080"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEY7042043.1"
                     /translation="REQDVEFFRTQGYLLPGRAMFAPDKLATLEQIFNDHLADKGDKL
                     SDELDTPHYRDARLFDFLLSDEVLDTVEPLIGPNIALWSSHFISKDPYTGRETPWHED
                     SAFWNGRFSSYDSIVTVWLALENPSTRENGCMRVVPGTHLTFGASEYVPTDMTTKTFH
                     AEIAEVDENQAVDFELQRGEFSLHDGRIVHGAKANTSSVRRTGYTMRYFPSSVRMFDN
                     EINKGWKIWLARGEDLAGNTYEPDPRVSA"
     gene            746..1666
                     /locus_tag="VH419_00085"
     CDS             746..1666
                     /locus_tag="VH419_00085"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEY7042044.1"
                     /translation="MELGFLTACLPGESLEQITRWAATAGYEALEVAAWPDRAGRDWE
                     ASHLDVEKFASADAEGVRRLLVDNGLTLSAVAFYENNLHEDPGTRETNHEHLRRCIDA
                     AQLLGCSLVGTFVGRDISLTVQENLRLAEKMLPPLVEYAAAREVRLVIENCPMEGWHP
                     DGYPANLAYSPELWRWLAGLGLYLNFDPSHLIWMGIDPVAALDECMDQVLHVQAKDAE
                     VDRRARNRFGVFGQLIDKRSPWASGWWRYRIPGLGEVDWPQLIARLRAAGFDGVVSVE
                     HEDPVWGGTPARVRQGLEIAHRTLEPLVHG"
     gene            1747..>3700
                     /locus_tag="VH419_00090"
     CDS             1747..>3700
                     /locus_tag="VH419_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042045.1"
                     /translation="MVDDLVDYRATILVWAAMGGGSLALPYLEQEAFGPVDARSRFYG
                     FVNDAEFIQECHKHGIKVLGIVFEAQGWEFPVELNDAEDQVLSLNELREVGHRDWLGL
                     REFSQNRYPRLWPPIEKYFPNGLVNSDGDPVTDLIDECVARDIHGRPCHAHWVECPDR
                     EHECYYMDRNNPVWREYLKAVIRIQIDAGVDGIQLDEAELPMGAFQYGACFCKDCMKG
                     FRSYLLGLPPSALDGVVEEAELEAFHYGEWLLRQGYDFKSDRESTPLFGDYYRFQCLE
                     IKKHFGELANYAREYGRSVGREVIVSGNFFNLEPMYLALADDVDLLITEMRNTTYQQP
                     EWYRYVAGFAGDKEVVVVENPYGGVVPELIQLLGEGKGHDLFRLSLYEAAALGATMTV
                     PYGSWMGSVIEDSFYAPHALATEIQGFLAEHEDLFSRSTVNDVAVVFSVESTRELIGK
                     ADASDNTTNRRDESVVVPYRAVTETMARSAVPFDVAIYADGVTAEDRASSAALARYRT
                     VVLPDCHLLTEGQTDALLGYLDGGGTVVVVDRFAETMPDARDAILDHPGTRRGDRNDL
                     DTLLPYGRQVVIDAPFAANIQQLSDGACAVHLINYDYVPETDAVRPVLDTRVGVTLDK
                     TYGRVTFIGSDGTRQTVDLERTAADGD"
BASE COUNT          643 a         1242 c         1209 g          606 t
ORIGIN      
        1 agggaacaag atgtcgagtt cttccgtacg cagggctacc tgttgcccgg gcgtgcgatg
       61 ttcgcgccgg acaagctcgc gacgctggag cagatcttca acgaccatct ggccgacaag
      121 ggcgacaagc tgtccgacga gctcgacaca ccgcactacc gcgatgcccg gctcttcgac
      181 ttcctgctgt ctgacgaggt tctcgacacg gtcgagccgt tgatcggtcc gaacatcgcg
      241 ttgtggtcga gccacttcat ctccaaggac ccctacaccg gccgcgagac gccgtggcac
      301 gaggacagcg cgttctggaa cgggcggttc agctcctacg acagcatcgt caccgtctgg
      361 ttggcgctcg agaacccgag cacgcgggag aacggctgca tgcgtgttgt tccgggcacg
      421 cacctcacgt tcggtgcctc cgagtacgtc ccgaccgaca tgaccaccaa gacgttccac
      481 gccgagatcg ccgaggtcga cgagaatcaa gcggtcgact tcgagctgca gcgcggcgag
      541 ttctccctcc acgacggacg catcgtgcat ggcgccaagg cgaacaccag cagcgtgcgt
      601 cgcacggggt acacgatgcg gtacttcccc tcgtcggtgc ggatgttcga caacgagatc
      661 aacaagggct ggaagatctg gcttgcccgt ggcgaggacc tggccggcaa cacctacgag
      721 cccgacccgc gagtctctgc ctgagatgga gctcggcttc ctgactgcct gcctgcctgg
      781 ggagtcgctc gagcagatca cccggtgggc ggccacggcc gggtacgagg cgctcgaagt
      841 ggccgcctgg ccggatcgag ccgggcgcga ctgggaggcg agccacctcg acgtcgagaa
      901 gttcgcatcc gcggacgccg aaggtgtgcg tcgattgctg gtcgacaacg gcctgacact
      961 ctcggccgtg gcgttctacg agaacaacct ccacgaggat cccggcacga gggagaccaa
     1021 ccacgagcac ctcagacggt gcatcgacgc ggcccagctg ctgggctgct ccttggtggg
     1081 aacgttcgtc ggacgggaca tctcgctgac ggtgcaggag aacctgcggc tcgccgagaa
     1141 gatgctcccc ccgctcgtcg agtacgccgc ggcccgcgag gtgcgcctgg tcatcgagaa
     1201 ctgcccgatg gaggggtggc acccagacgg ctacccggcc aacctcgcct actcccccga
     1261 actgtggcgc tggctggccg gattgggcct ctacctcaac ttcgacccgt cgcacctgat
     1321 ctggatgggc atcgacccgg tcgccgcgct ggacgagtgc atggaccagg tgctgcacgt
     1381 acaggccaag gatgccgagg tcgatcgccg cgcccgtaat cggttcggcg tgttcggaca
     1441 gctcatcgac aagcggtcgc cctgggcctc cggctggtgg cgctaccgca tcccggggct
     1501 cggcgaggtc gactggccgc agctgatcgc ccggttgcga gctgccgggt tcgatggcgt
     1561 ggtatcagtc gagcacgaag atccggtctg gggcggaact ccggcgcgcg tccggcaagg
     1621 cctcgagatc gcccaccgaa ccctcgagcc gctggtccac ggctgatgca ccgcggggtc
     1681 tacttcgacg cgtggtttcc gcgccagcac tgctaccacc cgagcctgcc accgcgacga
     1741 cttcgcatgg tggacgacct ggtcgactac cgggccacga tcctggtgtg ggcggccatg
     1801 ggcggcggct cgctggcact tccctatctc gagcaggagg cgttcgggcc cgtcgacgcc
     1861 cggtcacggt tctacggctt cgtcaacgac gccgagttca tccaggagtg tcacaagcac
     1921 gggatcaagg tcctcggaat cgtgttcgag gctcagggct gggagttccc ggtcgagctc
     1981 aacgacgccg aggaccaggt gctgtcgctc aacgagctgc gggaggtcgg acaccgcgac
     2041 tggctggggc ttcgcgagtt ctcgcagaac cgctacccca ggctctggcc gcccatcgag
     2101 aagtacttcc cgaacggcct cgtcaacagc gacggcgacc ccgtgaccga cctgatcgac
     2161 gagtgcgtcg cccgcgacat ccacggtcgt ccgtgtcacg cgcactgggt cgagtgcccc
     2221 gaccgtgagc acgagtgcta ctacatggac cgcaacaacc cggtctggcg cgagtacctc
     2281 aaggccgtca tccggatcca gatcgatgcc ggcgtcgacg ggatccagct cgacgaggcg
     2341 gagctgccga tgggggcctt ccagtacggc gcctgtttct gcaaggactg catgaagggc
     2401 ttccgcagct atctcctcgg ccttccgccg tctgccttgg acggcgtcgt cgaggaagcc
     2461 gagctcgagg cgttccacta cggcgagtgg ttgctgcggc agggctacga cttcaagtcc
     2521 gaccgtgagt ccaccccgct gttcggtgac tactaccgat tccagtgtct ggagatcaag
     2581 aagcacttcg gcgagctggc gaactacgcc cgcgaatacg gtcggtcggt ggggcgtgag
     2641 gtgatcgtct cggggaactt cttcaacctc gagccgatgt acctggccct ggccgacgac
     2701 gtggacctgc tgatcaccga gatgcggaac acgacgtacc agcaaccgga gtggtaccgg
     2761 tacgtcgcgg ggttcgccgg cgacaaggag gtcgtcgtcg tcgagaaccc gtacggcggt
     2821 gtcgtcccgg agctgatcca gctgctgggc gagggaaagg gtcacgacct gttccggttg
     2881 tctctctacg aagcggccgc gctcggcgcc acgatgacag tcccgtacgg ctcctggatg
     2941 ggcagcgtca tcgaggactc gttctacgca ccacacgccc tggccaccga gatccaaggc
     3001 ttcctcgccg agcacgagga cctgttcagc cggagcacgg tcaacgacgt agcagtcgtc
     3061 ttcagcgtcg agagcacccg cgagctgatc ggcaaggccg acgccagcga caacacgacg
     3121 aatcgacgcg atgagtcggt cgtcgtgcct tatcgcgcgg tcaccgagac catggcgagg
     3181 tcggctgtcc cgttcgacgt ggccatctac gccgacggcg tcacggccga agaccgcgcc
     3241 tcgtccgccg ctctggcccg ataccgcacc gtggtcctgc cggactgcca tctcctgacg
     3301 gagggccaga cggacgcgtt gctcggatac ctggacggtg gcggcaccgt ggtcgtcgtg
     3361 gaccgcttcg cggagaccat gcccgacgcc cgggacgcca tcctcgacca ccccggcacg
     3421 cgacgaggtg accgcaacga tctcgacacg ctcctcccgt acggacggca ggtcgtgatc
     3481 gacgcaccgt tcgcggccaa catccaacag ctctccgacg gggcatgcgc cgtgcacctg
     3541 atcaactacg actacgtccc ggagacagac gccgtgcgtc ccgtcctcga cacccgcgtt
     3601 ggtgtcacct tggacaagac gtacgggcgg gtcacgttca tcggcagcga cggcacccgg
     3661 cagaccgtcg acctcgagcg aaccgcggcg gatggtgacg
//
LOCUS       DATBFZ010000005         1679 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_1315430, whole genome shotgun sequence.
ACCESSION   DATBFZ010000005 DATBFZ010000000
VERSION     DATBFZ010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1315430"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="VH419_00095"
     CDS             <1..351
                     /locus_tag="VH419_00095"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY7042046.1"
                     /translation="QQRLSLYVAVIHDPALLLLDEPTAGLDPQSRRQLWGRIELIRQG
                     GGSILLTTHSMEEAQSVCDRVAIIDNGTLLTTETPRDMIAKHRNDPRVRQVARGEVTL
                     EDVFIGLTGSEIRD"
     gene            344..1114
                     /locus_tag="VH419_00100"
     CDS             344..1114
                     /locus_tag="VH419_00100"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY7042047.1"
                     /translation="MTSSRVTAGPPLGLVFRSLLRADFLVFLKHRRALIISMVLPVFV
                     LLSTSTSKATQRFGGVEFVIGLAIAYGLLSTSLIGYALTVARDREKGVFQRLRVTPAP
                     TWTIMTSRLAMQVLANLIISIAVVIIGSQIHHISPSIGQYALVLLVSILAGAVFLSIA
                     QALVGLVRSADSVQAIARVLFAILILLGLLGQSGALGSFWSSIARWTPVGVVMTLFAG
                     VLDLHAWHSRDTLSLLACLGYIIVFTGIGIRWFQWDAQ"
     gene            1123..1596
                     /locus_tag="VH419_00105"
     CDS             1123..1596
                     /locus_tag="VH419_00105"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY7042048.1"
                     /translation="MDTYNLFHDETAGDESDPPGFRAGSAQLAPSFGASRLAMTVYDL
                     PPGEAVCPYHFHWGDEEWMVVVSGTPTVRTPNGEQTLEPGDVVCFVVGPEGAHRVHNA
                     SDGAARVALFSNKHEFGIIEYPDSDKMGVWGRHNDALDHVVRRSPDLNYWDGERS"
     gene            complement(1613..1679)
                     /locus_tag="VH419_00110"
     tRNA            complement(1613..1679)
                     /locus_tag="VH419_00110"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1650..1652),aa:Met,seq:cat)
BASE COUNT          271 a          566 c          548 g          294 t
ORIGIN      
        1 cagcagcgcc tctccctgta cgtcgcggtc atccatgacc ccgcgctgct actcctcgac
       61 gagccgaccg ccggcctcga tccgcaatcg cgacgacagc tgtggggccg gatcgaactc
      121 atccggcagg ggggtggaag catcctgttg acgacgcact cgatggagga agcccagtcg
      181 gtctgcgatc gagtggcgat catcgacaac gggacgctgc tgacgaccga gacgccgcgc
      241 gacatgatcg cgaagcaccg caacgacccg cgggtccggc aggtcgctcg cggcgaggtc
      301 acgctcgagg acgtgttcat cggactcacc gggagcgaga tccgtgacta gctcgagggt
      361 cacggcgggg ccaccactgg gcctcgtgtt ccgttcgctg ctgcgcgcgg acttcctcgt
      421 cttcctcaag catcggcgcg cgctgatcat cagcatggtg ctgcccgtct tcgtcttgct
      481 cagcacgagc accagcaagg ccacccagag gttcggcggg gtcgagttcg tgatcgggtt
      541 ggcgatcgca tatgggctgc tctcgacttc gctgatcggc tacgcgctga cggtcgcgcg
      601 agaccgggag aagggcgtct ttcagcgact gcgcgtcacc ccggcgccca cctggacgat
      661 catgaccagc cggctggcga tgcaggtgct cgcgaacctg atcatctcga tcgccgtcgt
      721 gatcatcggc agccagatcc atcacatctc gccctcgatc gggcagtacg cgctggtcct
      781 gctggtctcg atcctcgcgg gagccgtgtt cctgagcatc gcccaggcgc tggtcggcct
      841 ggtcagatcc gccgacagcg tccaggccat cgcccgagtt ctgttcgcca tcctgatcct
      901 gctcgggctc ctcgggcaga gcggcgcgct gggcagcttc tggagctcga tcgcgcgctg
      961 gactccggtc ggggttgtga tgacgctgtt cgccggcgtg ctcgaccttc acgcctggca
     1021 cagccgcgac acgctttcgc tgctggcgtg cctcggctac atcatcgtgt tcaccggaat
     1081 cggcatccgc tggttccagt gggacgccca ataggagggg ccgtggatac atacaacctg
     1141 tttcacgacg agacagccgg cgacgagagc gatccgcccg gattccgtgc tgggtccgcg
     1201 caactcgccc cgagcttcgg agcgtcccgc ctcgcgatga ccgtgtatga cctgccgccc
     1261 ggggaggcgg tttgcccgta tcacttccac tggggcgatg aggagtggat ggtcgtggtg
     1321 tccggaacgc caaccgtccg cacgcccaac ggcgaacaga cgctcgagcc cggcgacgtc
     1381 gtgtgcttcg tggtgggtcc cgagggagcg caccgggtcc acaacgcgag cgacggcgcc
     1441 gcccgcgtcg ccctcttctc caacaagcac gagttcggga tcatcgaata ccccgacagc
     1501 gacaagatgg gcgtctgggg ccgccacaac gatgcgctcg atcacgtggt ccggcgcagc
     1561 ccggacctca actactggga cggcgagcgg tcctgacgag gcagatatca ggtggccctg
     1621 ccaggactcg aacctgggcg cgacggatta tgagtccgct gctctaccga ctgagctac
//
LOCUS       DATBFZ010000006         9716 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_332153, whole genome shotgun sequence.
ACCESSION   DATBFZ010000006 DATBFZ010000000
VERSION     DATBFZ010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 9716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9716
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332153"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2153
                     /gene="glgP"
                     /locus_tag="VH419_00115"
     CDS             <1..2153
                     /gene="glgP"
                     /locus_tag="VH419_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012922510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HEY7042049.1"
                     /translation="LGVGLLYRHGYFRQTLSREGWQQERYPIVDPDELPLTLLRELDG
                     SPTKIVVGLAGGQQLLAHVWVAQVGRVPLLLMDSDIEDNPATLRDVTDRLYGGGSDHR
                     LLQEVLLGVGGIRAIRAYCNVTGAPAPEVFHTNEGHAGFLGLERIREFIESDDLDFAS
                     AVEKARAGTVFTTHTPVPAGIDRFQRELIEMQFGGENSIPGMPVEDILALGAEDYDGG
                     DPTVFNMAVMGFRLSGRANGVSRLHGHVSREMFNGLWPGFDEDDVPIGSITNGVHAPT
                     WVAPEVFELAEKEAVLTSVENSEDWSAIDKIPSHALWETKRQLRARLVDDVRRRMRSS
                     WLKRGAAQAELGWTDSILDPDVLTIGFARRVPTYKRLTLMLRDPDRLKRLLTHPDHPV
                     QLVIAGKAHPADDTGKKLIQEMVRFSDDPAVRHRIAFLPNYDIAMAQALYPGCDVWLN
                     NPLRPYEACGTSGMKAALNGGLNLSILDGWWDEWYDGDNGWAIPSADGVDDPERRDDI
                     EAEALYDLLENEVAPRFYDVDGEKVPARWIEMVRHTLKSLGPKVLASRMVRDYVLTLY
                     VPASTATRRRDSAAAKRLAHWKAGVRQAWPHVRIEHVESSGVSDAPALGSTLAVRVYA
                     SLGDLFPEDVDVQVVHGRVAGDDQLHDPQVASLQHADVYEGGRHRFEGEVALEHTGPF
                     GYTVRILPANELLASPADVGLIAWPLDVNAAEVD"
     gene            2275..2559
                     /locus_tag="VH419_00120"
     CDS             2275..2559
                     /locus_tag="VH419_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042050.1"
                     /translation="MDSLPLVGAEVQRIDVAFTAVSAELAGVLSSLREVDLTSVGTPT
                     ADAAVATGMADLQASLTRLRGTADECVLVLRRHGTAESTEASPSDGGDAS"
     gene            2556..3455
                     /locus_tag="VH419_00125"
     CDS             2556..3455
                     /locus_tag="VH419_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042051.1"
                     /translation="MRADVVLGFEPTPSDPSALAAVREALRRTIRALVESRTQLDRLG
                     RTGSVWDGPAGAPVASLLRHYSRQLAALEESLIDCLQALETWIDRSAERQEHVAAIVT
                     SVAELAGVEGAEDRRTRLIATARELSVEHDRAAADLAAAFEELSSAVNQIAAVEEDLA
                     GELDRTLLALTAAVEDWLAAEAPELLRTAMSLGEVAGLTTVISELVGIAALGRDPGDA
                     EGVSEIVARSPGSHRLIRALRQQWLEMAPALPDASFAVSPTGGLADALAGRRQWSTGP
                     DKPGPDGSPGADRGQSAGDGNGN"
     gene            3458..4090
                     /locus_tag="VH419_00130"
     CDS             3458..4090
                     /locus_tag="VH419_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042052.1"
                     /translation="MVLLIEPRSASAPAVVLDVPADFHQVPLASDTEDRTAAQLQVLE
                     DMGLSDTGQREALSLYLEALSKRLSGGPVLATAFCAVRVNDHPSTATLTVGLHTMRTA
                     DRGLAVLGAAEAMRREGRYANVAIVEVGPHPAVTAVAERATTPEDTVTGEVGATLREL
                     SVLVPVPGHEQAVIVTLSTPCLEDWDIYQRLVLDICRSLRVQSALGDAIS"
     gene            complement(4166..6313)
                     /gene="glgX"
                     /locus_tag="VH419_00135"
     CDS             complement(4166..6313)
                     /gene="glgX"
                     /locus_tag="VH419_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HEY7042053.1"
                     /translation="MSSVEGPVERPVVWPGSHDALGARFDGTGTNFAVWAPDATAVDL
                     CLFDDAGVEVRLALPEHTLGVWHGFVRGVAPGQRYGFRVSGPWDPDHGHVFNPDKLLL
                     DPYARAIDGDMVVDRALAIVDRDRQPMAGDTAGLTPRSVVVGDAPFDWGEDRRPEIPW
                     RHTVIYEAHVKGLTHRHPDVPAELRGTFAAMGHPATIHYLKDLGVTSIELLPVHHFVS
                     EPVLLERGMVNYWGYNTVGFFAPHAAYSASGSRGQQVTEFRQMVKNLHAAGLEVILDV
                     VYNHTGEGGPNGPALCFRGYDDGSYYRTDGWGRYADVTGCGNTVNVSEPQVLQLVMDS
                     LRYWVTEMHVDGFRFDLASALTRNGPNVDLHAPFLTAVHQDPVLRRVKLIAEPWDATS
                     DGYLVGRFPPPWCEWNDKYRDAVRDFWRGHGGGVRDLASRLSGSSDLYADDGRLPFST
                     VNFVTAHDGFTLRDLVSYNRKHNEANGEQNTDGTDNHRSWNCGVEGETTDDTVIALRR
                     RQSANLLATLLLSTGVPMITAGDERGRTQGGNNNAFCQDNPTSWFPWEGLDKDWLHLD
                     RLTRLLLRLRADHPVLRQRHFFAGTPLGSNGRKDISWLRPDGSEMTVDDWQDPTRSTL
                     GVFLAGDALRGVNAVGERTHDTSYVLWLHAGTAPVDVTLPKALADAYVTVLRTDQDLD
                     ALDDLFDSGERLRAGSTVTLLDRTFALFEALTH"
     gene            6390..7169
                     /locus_tag="VH419_00140"
     CDS             6390..7169
                     /locus_tag="VH419_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007031679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEY7042054.1"
                     /translation="MADFARLEVSEGVGTLRLDRPPMNALDVQMQEEIRAVAAEATDR
                     DEVRAVVVYGGEKVFAAGADIKEMAAMSYTDMVKRSGALQSAFTAVARIPKPVVAAVT
                     GYALGGGCELALCADVRIAADDAKIGQPEILLGVIPGAGGTQRLSRLVGPAKAKDIIF
                     TGRFVAAEEALSIGLVDKVVPAAEVYDAAVSWAAAFAAGPAYALRAAKEAIDRGLETD
                     LETGLQIERQQFAALFATEDRTAGMTSFVEKGPGKAVFEGR"
     gene            7390..8175
                     /locus_tag="VH419_00145"
     CDS             7390..8175
                     /locus_tag="VH419_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEY7042055.1"
                     /translation="MKIVVCVKYVPDASADRTFGSDNTVDRVGVDGVLSELDEYAVEE
                     ALKIRDSGVDDVEIVVVTMGPGEARAAILKSLQMGADAGVHVKDDAIHGSDAIATSLV
                     LANAIERQGPDIVLCGMASTDGSMSVVPAMLAERLSLPQVTFASEVKVDGSTVQVRRD
                     GDSATETIEASTPLLISVTDQANEPRYPSFKGIMAAKKKPVEELTIADLGIDANDVGT
                     AAAWTEVTGVEARPPRTAGTIVSDTDGSGADQLVEFLAGQKFI"
     gene            8199..9155
                     /locus_tag="VH419_00150"
     CDS             8199..9155
                     /locus_tag="VH419_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020671078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEY7042056.1"
                     /translation="MAEILALVDHVDGTVRKTTAELLTLARRLGEPSAVFIGSGLDTA
                     RESLKRFGAAKVYVMDSADLTGFLVAPKAEALAQLVDSKRPGAVLIASNAEGKEIAGR
                     LAIKTSSGVITDAVDVQSGENGPVTTQSVFAGNFTVTARVTKGTPIITVKPNSVTPEA
                     VDGTAAEESVDVDVSDVAKTAKITQSQTRQATGRPELTEASIVVSGGRGTGGNFEPVE
                     AFADALGAAVGASRAAVDSGWYPHSYQVGQTGKTVSPQLYVATGISGAIQHRAGMQTS
                     KTIIAINKDEEAPIFELVDFGVVGDLHAVLPKATEQVNARKG"
     gene            9239..>9716
                     /locus_tag="VH419_00155"
     CDS             9239..>9716
                     /locus_tag="VH419_00155"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008361208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HEY7042057.1"
                     /translation="MDESTALLVVVMGVSGTGKSTVGERLADRLRVPFADADEFHPPA
                     NLEKMAAGTPLTDEDRWPWLEAIGRWLGEHAEEGAVATCSALRRVYRDVLRQRAAGIW
                     FLYLDGNNDVIAQRVSSRPLHFMPASLLQSQFDTLEPPDPDERAVAVDTGQPPDEIV"
BASE COUNT         1514 a         3220 c         3460 g         1522 t
ORIGIN      
        1 tcctcggcgt cgggctgctc taccggcacg gctacttcag gcagacgctg tccagggagg
       61 ggtggcaaca ggagcgctat ccgatcgtcg acccggacga gctgccattg acgctgctgc
      121 gcgagctcga cggcagcccg acgaagatcg tggtcggact cgctggcggt cagcagctcc
      181 tcgcccacgt gtgggtggct caggtcggcc gggttccgct gctgctgatg gactccgaca
      241 tcgaggacaa ccccgccacg ctgcgcgacg tcaccgaccg gctctacggc ggcggctccg
      301 accatcgact gctccaggag gtgctgctcg gcgtcggcgg gattcgtgcc atccgcgcct
      361 actgcaacgt caccggtgcg ccggcccccg aggtcttcca caccaacgag gggcatgccg
      421 gcttcctggg tctcgagcgc atccgggagt tcatcgagtc cgacgacctg gacttcgcca
      481 gcgcggtgga gaaggcgcga gccgggacgg tgttcacgac gcacaccccg gtgccggccg
      541 ggatcgaccg gttccagcgc gagctcatcg agatgcagtt cggcggcgag aactccatcc
      601 cgggaatgcc ggtcgaggac atcctcgccc tcggggcgga ggactacgac ggtggcgatc
      661 cgaccgtctt caacatggcc gtgatgggat tccggctctc cggccgtgcc aacggcgtca
      721 gccggctgca cggccatgtg tcccgggaga tgttcaacgg gctctggccc ggcttcgacg
      781 aggacgacgt gccgatcggc tcgatcacca acggcgtcca cgcgccgacg tgggtggcac
      841 cagaggtgtt cgagctggcc gagaaggagg ccgtcctcac gtcggtcgag aactccgagg
      901 actggagcgc catcgacaag atcccctccc atgcgctctg ggagaccaag cgccagctac
      961 gggctcggct ggtcgacgat gtgcggcggc gcatgcgctc gtcctggctc aagcgcggtg
     1021 cggcccaggc agagctcggc tggaccgact cgatcctcga tccggacgtg ctcacgatcg
     1081 ggttcgcgcg acgcgtgccg acgtacaagc ggctgacgtt gatgctgcgc gacccggatc
     1141 ggctcaagcg gctgttgacg caccccgacc acccggtgca gctcgtcatc gcggggaagg
     1201 cgcaccccgc ggacgacacc ggcaagaagc tgatccagga gatggtgcgg ttctccgacg
     1261 acccggcggt gcggcaccgg atcgccttcc tgcccaacta cgacatcgcg atggcccagg
     1321 cgctgtatcc gggttgcgac gtctggctga acaacccgct gcgaccgtac gaggcgtgcg
     1381 gcacgtcagg gatgaaggcc gccctcaacg gtgggttgaa cctgtcgatc ctcgacggct
     1441 ggtgggacga gtggtacgac ggcgacaacg gctgggcgat cccgtcggcg gacggcgtgg
     1501 acgatccgga gcgccgcgac gacatcgagg cggaggcgtt gtacgacctg ctcgagaacg
     1561 aggtggcgcc gcgcttctac gacgtcgacg gggagaaggt gccggctcga tggatcgaga
     1621 tggtgcgcca cacgctgaag tcgctcgggc ccaaggtgct cgcgtcgcgg atggtccgtg
     1681 actacgtcct gacgctgtac gtgcccgcct cgaccgccac ccgtcggcgg gactcggccg
     1741 cggcgaagcg actcgctcac tggaaggcgg gggttcgtca ggcgtggccg cacgtccgga
     1801 tcgagcacgt cgagtccagt ggcgtcagtg acgcgcccgc gctgggatcg accttggccg
     1861 tccgggtgta cgcgtccctc ggggacctct tcccggagga cgtcgacgtc caggtcgtgc
     1921 acggccgcgt ggcgggcgac gaccagctgc acgatccgca ggtggcgagt ctgcagcatg
     1981 cagacgtgta tgagggcgga cggcaccggt tcgagggcga ggtcgcgctc gaacacaccg
     2041 gaccgttcgg ctacacggtg cgcatcctgc cggcgaacga gctgctcgcg agccccgcgg
     2101 acgtcgggct catcgcgtgg ccgctcgacg tgaacgctgc cgaagtcgac tgacggcacg
     2161 tcggtttccg tctcgacatc gggccacggt gacccgctga cgcggcagga tggacgaagt
     2221 ttcactcacg tgggccgtcc tgggtccgcg tggccctcgt gatcggagtc agccgtggac
     2281 tcgctcccgc tcgtcggcgc cgaagtgcaa cggatcgacg tcgcgttcac ggccgtgtcc
     2341 gcggagcttg ccggtgtgct gtccagcctg cgcgaggtcg acctgaccag cgtcggtacg
     2401 ccgacggcgg atgcggcggt cgccaccggc atggcagacc tccaggccag cctgacgagg
     2461 ttgcggggca cggctgacga gtgcgtgctg gtgttgcgcc gacacgggac ggccgagagc
     2521 accgaggcct cacccagtga cggtggtgat gcgtcgtgag agcggatgtc gtcctcggat
     2581 tcgagccgac gccgagcgac ccgagcgccc tcgccgctgt gcgcgaggcc ctgcgccgga
     2641 cgattcgggc cctggtggag tcgcgcacac agctggaccg gctgggcagg acgggttcgg
     2701 tctgggacgg gccggccggc gccccggtgg cgtcgctgct gcggcactac tcacgtcagc
     2761 tggcggcgtt ggaggagtcg ctgatcgact gccttcaggc actcgagacc tggatcgacc
     2821 gttcggcgga gcggcaggaa cacgttgccg ccatcgtcac gtcggtggcc gagctcgccg
     2881 gcgtcgaggg cgccgaggac cggcgtacgc ggctgatcgc caccgcccgc gagctcagcg
     2941 tcgagcacga ccgcgcggcc gccgatctcg ccgctgcatt cgaggagctg tcgagcgcgg
     3001 tcaaccagat cgcggcggtc gaggaagacc tggccggcga gctcgaccgg accctgctgg
     3061 ctctgactgc cgccgtggag gactggctcg cggcggaggc acccgagctg ttgcggacag
     3121 cgatgtcgtt gggcgaggtc gcgggtctca cgacggtcat ctccgagctc gtcgggatcg
     3181 cagcgttggg gcgtgacccc ggcgacgcgg aaggggtcag tgagatcgtc gcgcgcagcc
     3241 ctggttcgca ccggttgatc cgcgctttgc gacaacagtg gctcgagatg gcaccggcgc
     3301 tccccgacgc gtcgttcgcc gtgagcccga ccggtggtct cgcggatgct ctggccgggc
     3361 ggcgtcaatg gtcgaccggt ccggacaagc ccgggcccga cggcagtcca ggtgcggacc
     3421 gcggccagtc ggccggagac gggaacggga actgaccgtg gtgctgctga tcgaaccacg
     3481 cagtgcgagc gccccggccg tcgttctcga cgtaccggcg gacttccacc aggttccact
     3541 ggcgtcagac accgaagacc ggaccgccgc gcaactgcag gtgctggagg acatgggcct
     3601 gtcggacacc ggccagcggg aggcgttgtc gctttacctc gaggcactct cgaagcggtt
     3661 gtcgggtggg ccagtgctgg ccacggcgtt ctgtgccgtt cgggtcaacg accatccctc
     3721 gacggccacg ttgacggtgg ggttgcacac gatgcgcacg gccgaccgcg ggcttgcggt
     3781 gctcggtgcg gccgaggcga tgcggcgcga ggggcggtac gccaacgtcg cgatcgtcga
     3841 ggtcggtccg catccggcgg tgacggcggt cgcggagcgg gcgacgactc ccgaggacac
     3901 ggtcactggc gaggtcggcg ccacgctgcg tgagctctcg gtgctggtac cggtgcctgg
     3961 ccacgagcag gctgtcatcg tcacgttgtc gacgccgtgt ctggaggact gggacatcta
     4021 ccagcggctg gtcctcgaca tctgtcggtc gcttcgcgtc cagtccgctc tcggcgacgc
     4081 catctcctga cctgcgccga ggtggcgatc gctgctcccg agtcggcgtg tcttgaacga
     4141 aacctgccac ctcggcggga ggtggtcagt gggtgagggc ttcgaagagg gcgaaggtgc
     4201 ggtcgaggag cgtcacggtc gagccggcac ggagccgttc gccactgtcg aagaggtcgt
     4261 cgagtgcgtc gaggtcctgg tcggtgcgta ggaccgtcac gtacgcgtcg gcgagcgcct
     4321 tcggaagcgt gacgtccacc ggtgctgttc cagcatgcag ccacaggacg tacgacgtgt
     4381 cgtgcgtccg ttcgccgacc gcgttgaccc cgcgcagcgc gtctccggcc agaaacaccc
     4441 ccagtgtcga gcgggtcggg tcctgccagt cgtccacggt catctccgaa ccgtcaggtc
     4501 tcagccagct gatgtccttg cggccgttgg accccaacgg cgtaccggcg aagaagtgcc
     4561 gctgtcggag caccgggtgg tcggcgcgca accggagcag caaccgggtc agccgatcga
     4621 ggtgcagcca gtccttgtcc aggccttccc acgggaacca ggaggtcggg ttgtcctggc
     4681 agaaggcgtt gttgttgccg ccctgggtgc gcccgcgctc gtcgcctgcg gtgatcatcg
     4741 ggactccggt ggacagcagc aaggtggcga gcaggttcgc cgactggcgg cggcggagcg
     4801 cgatgacggt gtcgtccgtg gtctcgccct cgacgccaca gttccacgag cggtggttgt
     4861 ccgtgccgtc ggtgttctgc tcgccgttgg cctcgttgtg cttgcggttg tacgacacga
     4921 ggtcgcgcag tgtgaagccg tcgtgcgcgg tgacgaagtt gaccgtcgag aacggcagcc
     4981 gcccgtcgtc ggcgtacagg tcgctggacc cggacaggcg tgacgccagg tcgcgtacgc
     5041 cgccgccatg gccacgccag aagtcgcgga cggcgtcgcg gtacttgtcg ttccactcgc
     5101 accacggcgg cgggaaccgc ccgaccaggt agccgtcgct ggtcgcgtcc cacggctcgg
     5161 cgatcagctt gacccgccgc agcaccgggt cctggtgcac cgccgtcagg aatggtgcgt
     5221 gcaggtcgac gttcgggccg ttgcgtgtca gcgccgaggc caggtcgaac cggaacccgt
     5281 cgacgtgcat ctccgtgacc cagtagcgca gcgagtccat cacgagctgg agcacctgtg
     5341 gttcggagac gttgacggtg ttgccgcatc cggtcacgtc ggcgtatcgc ccccacccgt
     5401 cggtccggta gtaggacccg tcgtcgtacc cgcggaagca cagcgcagga ccgttcgggc
     5461 cgccctcgcc ggtgtggttg tagaccacgt cgaggatcac ctcgaggccg gccgcgtgca
     5521 ggttcttcac catctgccgg aactcggtga cctgctgacc gcgcgagccg ctggcggagt
     5581 acgccgcatg gggcgcgaag aagccgacgg tgttgtagcc ccagtagttg accattccgc
     5641 gctcgagcag gacgggttcg gagacgaagt ggtggacggg tagcaactcg atcgacgtga
     5701 cgccgaggtc cttcaggtag tggatcgtcg cggggtgtcc catcgccgcg aacgtgccac
     5761 gcagctctgc cggaacgtcg gggtgccggt gggtcagccc cttgacgtgc gcttcgtaga
     5821 tgaccgtgtg tcgccacggg atctcgggcc ggcggtcctc cccccagtcg aagggagcgt
     5881 cgccgaccac gacgcttcgc ggggtcaggc cggcagtgtc gccggccatc ggctgccggt
     5941 ctcggtcgac gatcgcgagt gcgcggtcga ccaccatgtc gccgtcgatg gcgcgcgcat
     6001 acgggtcgag caacagcttg tccgggttga acacgtggcc gtggtcggga tcccacggac
     6061 cgctgacccg gaagccgtag cgctggcccg gcgcgacacc cctgacgaac ccgtgccaga
     6121 cgccgagcgt gtgctcgggc agcgccagcc gtacctcgac gccggcgtcg tcgaacaggc
     6181 acaggtcgac cgcggtcgcg tccggcgccc acaccgcgaa gttggtcccg gtcccgtcga
     6241 atcgagcgcc cagggcgtcg tggctccccg gccacaccac cggccgctcg accggccctt
     6301 cgacggaact catgcaccca tcctgccgaa cgcggcggtc gtcggtgccg gatcgttgcc
     6361 gaatcgacgt ggtactacgc tccccggtca tggctgattt cgcgcgactc gaggtctccg
     6421 aaggtgtcgg cacgctccgt ctggaccggc cgccgatgaa cgcgttggac gtgcagatgc
     6481 aggaggagat ccgggctgtc gccgcggagg ccaccgaccg cgacgaggtg cgagcggtgg
     6541 tcgtgtacgg cggggagaag gtcttcgcgg ccggcgccga catcaaggag atggcggcga
     6601 tgtcgtacac ggacatggtc aagcggtcgg gggcactcca gtccgcgttc accgccgtcg
     6661 cccggatccc gaagccggtg gttgcggccg tgacgggcta cgcgctcggc ggcggctgcg
     6721 agctggcgct gtgcgccgac gtccggatcg ccgccgacga cgcgaagatc ggtcagccgg
     6781 agatcctgct cggcgtcatc cccggcgctg gggggacaca gcggctcagt cggctggtcg
     6841 gtccggcgaa ggccaaggac atcatcttca ccggtcggtt cgtcgcggcc gaagaggcgc
     6901 tttcgatcgg gctcgtggac aaggtcgttc ccgctgccga ggtgtacgac gcggctgtgt
     6961 cctgggccgc ggcgttcgcg gcaggcccgg cgtacgcgct gcgggccgcg aaggaggcga
     7021 tcgaccgtgg cctggagacc gatctcgaga ccggcctgca gatcgagcga caacagttcg
     7081 cggcattgtt cgcgacagag gatcgcaccg ccgggatgac gtcgttcgtg gagaaggggc
     7141 caggcaaggc cgtcttcgaa ggtcgctgac accacctccg cgccgagtgg acgttcgcgg
     7201 tgcaaacatc gcaaaccgac tctttccatc ggaggtttgc gatgtctaca ggccgggtgg
     7261 gctgtaaaca tcgcaaaccc ccaagggctg cggcggtcgg ctgacgggta gcctgcgggc
     7321 ctgagagcac tccggacact cactccggac actactgcgg acactggaga cgggaggccc
     7381 agctcggcca tgaagatcgt tgtctgcgtc aagtacgtgc ccgacgcctc ggccgaccgg
     7441 acgttcggct ccgacaacac cgtcgaccgc gtgggcgtcg acggcgtgct gtccgagctc
     7501 gacgagtacg ccgtcgagga ggcattgaag atcagagact cgggtgtcga cgacgtcgag
     7561 atcgtcgtcg tcacgatggg tccgggggag gcgcgcgctg cgatcctgaa gtcgcttcag
     7621 atgggtgcag acgccggtgt ccacgtcaag gacgacgcga tccacggctc cgacgccatc
     7681 gccacgtcgc tggtactcgc caacgcgatc gaacgacaag gccccgacat cgtgctgtgc
     7741 ggcatggcct cgaccgacgg ctcgatgagc gtcgtacccg cgatgctggc agagcgactc
     7801 tcgctgccgc aggtgacctt tgcgtcggag gtcaaggtcg acggctccac cgtgcaggtg
     7861 cgtcgagacg gcgactccgc caccgagacg atcgaggcga gtacgccgct gctgatcagc
     7921 gtcaccgacc aggccaacga acctcggtac ccctcgttca agggcatcat ggccgccaag
     7981 aagaagcccg tcgaggagct cacgatcgcc gacctgggca tcgatgcgaa cgacgtgggt
     8041 acggctgccg cctggaccga ggtgacaggt gtcgaagccc gcccgccgcg tacggcgggg
     8101 acgatcgtgt ccgacaccga cggctccgga gccgaccagc tggtcgagtt cctggccggc
     8161 cagaagttca tctgagtccg agaacggagc gcacatcgat ggcagagatc ctcgccctcg
     8221 tcgatcacgt ggatggcacg gtccgcaaga ccacggccga gctgttgacg ctggcgcgac
     8281 gcctggggga gccatcggcc gtcttcatcg gatccgggct cgacacagca cgcgagtcac
     8341 tgaagaggtt cggcgcggcg aaggtctacg tcatggactc cgccgatctc accggtttcc
     8401 tcgtcgctcc gaaggcggaa gcgctggccc agctcgtgga cagcaagcgg ccgggagccg
     8461 tgctcatcgc gtcgaacgcc gaaggcaagg agatcgccgg tcgcctcgcg atcaagacca
     8521 gctccggcgt catcaccgat gccgtcgacg tgcagtcggg tgagaacggt ccggtgacca
     8581 cccagtccgt gttcgccggc aacttcaccg tcacggcgcg agtcacgaag ggaacgccga
     8641 tcatcacggt caagcccaac tccgtgacac cggaggccgt tgacggcacc gctgcagagg
     8701 agtccgtcga cgtcgacgtc agtgacgtcg ccaagaccgc aaagatcacg cagtcgcaga
     8761 cccgtcaggc gaccggtcgc ccagagctga ccgaggcgtc catcgtcgta tccggtggtc
     8821 gcggcaccgg cggcaacttc gagccggtcg aggcgtttgc cgacgctctg ggcgccgcgg
     8881 tcggagcctc ccgtgcggcc gtcgactctg gttggtatcc gcactcctac caggtcggcc
     8941 agaccggcaa gaccgtgtca ccacagttgt acgtcgccac cggcatctcg ggtgcgatcc
     9001 agcaccgcgc ggggatgcag acctccaaga ccatcatcgc catcaacaag gacgaggagg
     9061 cgccgatctt cgagctcgtc gacttcggcg tcgtcggcga cctgcacgcg gtgttgccca
     9121 aggccaccga gcaggtcaac gcgcgcaagg gctgacgcga ctgcccgttt ccgcgcagtg
     9181 cggaaccacc gcggtggtcg ggaagcatga gcaccggctg cgcgggtatg ccctgcctat
     9241 ggacgagtcc acggcgctcc tggtcgtcgt gatgggagtc tcgggtaccg gcaagtcgac
     9301 ggtgggagag cgcctggcag accgcctccg ggtgccgttc gccgacgccg acgagttcca
     9361 tccacccgcc aacctcgaga agatggccgc cggcacgccg ctgacagacg aggatcgctg
     9421 gccgtggctg gaggcgatcg gccgctggct tggcgaacac gccgaggagg gtgcggtcgc
     9481 cacctgctcg gcgcttcgcc gtgtctaccg tgacgtgctg cgccagcggg ccgccggcat
     9541 ctggttcctc tacctcgacg gcaacaacga cgtgatcgcg caacgggtgt ccagccgccc
     9601 cctccacttc atgccggcgt ccctgctgca gtcgcagttc gacacgctgg agccgccgga
     9661 tccggacgaa cgtgccgttg cggttgacac cggtcagcca ccggacgaga tcgtcg
//
LOCUS       DATBFZ010000007         4262 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_5908452, whole genome shotgun sequence.
ACCESSION   DATBFZ010000007 DATBFZ010000000
VERSION     DATBFZ010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 4262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4262
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5908452"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            226..1920
                     /locus_tag="VH419_00160"
     CDS             226..1920
                     /locus_tag="VH419_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008363491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEY7042058.1"
                     /translation="MTDPRPARRVDAIADQFVADYAALDPIAATYFGISGHDDQLTDL
                     SPGGFDARRELLVRALGRMQATEPVDELEVAAKASFVERQTVALEQYAAGTPQSQINV
                     VTSGLHSIRSIFDLMSTDGEQAWTAIDTRLAHIPATVEGYQHTLSRAADEGHVAAKRQ
                     ISLVAEQIKAWTGQVGLGGDFFATFVGQSDGQPASLRKRLDEHAAQSSAAFADFGRFL
                     SEELLPRGRDEDAVGRDAYVPESRSFLGALIDLDETYAWGWDELARIEDEMARVADKI
                     QPGATVDEAVAVLDADPARRIESKEVFRDWMQLLADRTVAEMAGTHFDIPGPVRRIEC
                     CLAPTHDGGIYYTGPSEDFERPGRMWWAVPDDQMSFSTWREVTTVYHEGVPGHHLQVA
                     QTAYRSELLNRWQRTMCWVSGHGEGWALYAERLMDDLGYLDDPGDKLGMLDGQGMRAA
                     RVIIDIGMHLGLDIPRDNPFGFHPGEKWTAELCLEFLRQHCRMDDAIVQYEVNRYLGW
                     PGQAPSYKVGERIWLQARDEVRERKGAAFDLKDFHRHALDLGSLGLDPLRAALARL"
     gene            1947..2561
                     /locus_tag="VH419_00165"
     CDS             1947..2561
                     /locus_tag="VH419_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020576836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HEY7042059.1"
                     /translation="MTSARVRLVLASASPARRATLRAAGVCPEVVVSDVDETGYTAED
                     PEALALRLAVAKATAVAQLSVAAGAVVVGCDSVLDLDGVALGKPVDAEEAVRRWTSMR
                     GRSGTLVTGHCVVDTRSGKQVEAVARTQVTFADVTDAEIEAYVATGEPLAVAGAFTID
                     GLGGAFVTGIAGDHHNVVGLSLPLLRRMLLDLDIPWTDLWSPTS"
     gene            complement(3288..>4262)
                     /locus_tag="VH419_00170"
     CDS             complement(3288..>4262)
                     /locus_tag="VH419_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012919075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY7042060.1"
                     /translation="CVAIEEIGRVDQSMGITLEAAVGLGINPILTYGSDEQKQTWLPD
                     LVTGTTLAGFGLTEPGSGSDAGATRTKADLVDGEWVVNGSKQFITNSGSDITSVVTVT
                     ARTGVSTSPTTGKDRAEVSAIIIPTGTPGFTAEPAYDKLGWHASDTHPLSFDDVHVPE
                     SNLLGERGKGYAQFLATLDDGRVAIAALAVGCIQACLDMSVQYAGERTTFDVPIGSKQ
                     GVAFQIADLKVMAEAARGLTYKAAAIKDAGRSTKEFKEAASIAKLYSTEAAVTATRIA
                     TQVFGGYGFMEEYPVARFYRDAKVLEIGEGTSEVQRMLIARALGLPVE"
BASE COUNT          696 a         1459 c         1426 g          681 t
ORIGIN      
        1 acaggatcgc gcgtggtgcg tcgccagtga gatcgacttc gactcgacct tggtcgcggg
       61 ttcggaggtt ctcatcggcg agatcatcgg acatgccgac ctcgaggcgt ttcctgtcgc
      121 gcccgccgac gacctcagcc atcgcggcga caccgtcaac ccgcgactgt gacgtcaact
      181 gaactggctc gtcgcttcgt gatccggctg cgctaggttg actcggtgac cgacccacga
      241 ccagcgcgac gtgtcgacgc gatcgccgac cagttcgtcg ccgactacgc ggccctcgac
      301 ccgatcgctg ccacgtactt cgggatcagc gggcacgacg accagctcac cgacctctcg
      361 cccgggggct tcgacgcacg tcgcgagctg ctggtgcgag cgctcggccg gatgcaggcg
      421 accgaacccg tcgacgagct ggaggtggca gccaaggcgt ccttcgtcga acgacagacc
      481 gtcgcgctcg agcagtacgc cgcgggaacg cctcagtcgc agatcaacgt cgtcacgagt
      541 gggttgcaca gtatccgcag catcttcgac ctcatgagca ccgacggcga gcaggcatgg
      601 accgccatcg acacgcggct cgcccacatt cccgccacgg tcgagggcta ccagcacacc
      661 ctgtcgaggg ctgccgacga gggacacgtg gccgccaagc gacagatctc cctggtcgct
      721 gagcagatca aggcctggac cggccaggtc ggcctgggcg gcgacttctt cgcgacgttc
      781 gtcggccagt cggacgggca gcccgcgtcg ctgcgcaaac ggctggacga gcacgctgcc
      841 cagagcagcg cggcgttcgc cgacttcggc cggttcctca gcgaggagct gctgccacgc
      901 ggtcgagacg aggacgccgt cgggcgagac gcctacgttc cggagtcacg atcgttcctc
      961 ggtgccctca tcgacctcga cgaaacctac gcctgggggt gggacgaact cgcgcgcatc
     1021 gaggacgaga tggcccgggt ggccgacaag atccagcccg gtgccaccgt ggacgaggct
     1081 gtcgccgtgc tcgacgccga ccccgcccgc cgaatcgaga gcaaggaggt gttccgcgac
     1141 tggatgcaac tgctcgccga ccgaaccgtc gccgagatgg ccggcacaca cttcgacatc
     1201 cccggaccgg tacgccgcat cgagtgctgc ctcgccccta cgcacgacgg tggcatctac
     1261 tacaccggac cgagcgagga cttcgagcga ccgggtcgga tgtggtgggc cgtgcctgac
     1321 gaccagatgt cgttctcgac gtggcgtgag gtgaccacgg tctaccacga gggcgtgccg
     1381 ggtcatcacc tgcaagttgc gcagacggcg taccgcagcg agctgctgaa ccgttggcag
     1441 cgcacgatgt gctgggtttc gggccacggt gaaggatggg cgctgtacgc cgagcggctg
     1501 atggacgacc tcgggtacct cgacgacccc ggcgacaagc tcgggatgct cgacgggcag
     1561 ggtatgcgag ccgcccgggt catcatcgac atcggcatgc atctcgggct ggacatcccg
     1621 cgcgacaacc cgttcgggtt ccacccgggt gagaagtgga ccgctgagct ctgcctggag
     1681 ttcctgcgcc agcactgccg gatggacgat gccatcgtcc agtacgaggt caaccgctac
     1741 ctgggatggc ccggccaggc gccgtcgtac aaggtcggcg aacggatctg gctgcaggcc
     1801 cgcgacgagg tccgcgaacg caagggcgcg gccttcgacc tcaaggactt ccatcgccac
     1861 gcgctcgacc tgggctcgct gggtcttgac ccgttgcgag ccgcgctcgc ccgcttgtga
     1921 ccgctgcgga gggacgcgag ggaatgatga cctccgcgcg ggtgcggctc gtcctcgcct
     1981 ccgcctctcc cgcgcggcgt gcgacgctgc gcgcggccgg cgtctgcccc gaagtcgtcg
     2041 tgtccgacgt cgacgaaacc ggctacaccg cggaggatcc ggaggcgctc gcgctccgcc
     2101 tggcggtggc gaaggcgacg gcggtggcgc agctgtccgt cgcggccggt gccgtcgtgg
     2161 tggggtgcga ctccgtcctc gacctcgacg gtgttgcttt gggcaagccg gtcgacgccg
     2221 aagaggcggt acgccgctgg acgtcgatgc gtggtcgctc cggcaccctg gtgaccgggc
     2281 attgcgtggt ggatacccgg tcgggcaagc aggtggaggc ggttgcccgc acgcaggtca
     2341 ccttcgccga cgtgacggac gctgagatcg aggcctacgt cgcaaccggc gagccgttgg
     2401 cggtggcagg ggcgttcacg atcgacggcc tgggtggcgc gttcgtcacc gggatcgccg
     2461 gtgaccacca caacgtcgtg ggcctctccc tgcccctgct ccgccggatg ctcctcgatc
     2521 tggacatccc ctggaccgac ctctggtccc ccaccagttg atgcgtggcc cacgctgaca
     2581 gcgctgctgt gaggttcgcg atgtttacag cccaaaaggc gtgtaaacat cgcaaaccct
     2641 cgataggcac agtgggtctg ccgtgtggac atcgcagact gcagccgctc ggcgaaggat
     2701 gtccgaaacc cgccagtcac acatcaggca caaggaacgg aacgtcggct gacgagccca
     2761 gcgggcacga acgtctcccg cgcttcgctg ccatccccca gccgaccatc caggccgagg
     2821 tggtttgcct cggtagccat ccaaacggac aggtagccag gcagatctgc ctcgggacgg
     2881 gtggacttgc acgggtagcc atgcaaaccg ccgctcggca accggcaatg tccgaaaccc
     2941 gcgagtcgcg ggcaccgccg gcggacggta cgccggccga cagcctcagc gagacaccaa
     3001 cgtctcccgc gcttcgctgc catcccacct cagccgacca cgtaggccga ggtggtttgc
     3061 ctcggtagcc atccaaacgg acgggtagcc aggcagatct gcctcgggac ggatgggttt
     3121 gcacgggtag ccatgcaaac cgcttggccg ccacaggtgt gaccgtcttg actattcgac
     3181 cgaccacctg ggccgagatg gcagctttcg tccagggcac gccgacggga gaacgaaaat
     3241 cgccacctcg actggccacc tggacgccac aggtgtgacc gtcttgacta ttcgactggt
     3301 aggcccaggg cgcgggcgat gagcatgcgc tggacctccg aagtgccttc gccgatctcg
     3361 agcaccttcg cgtcccgata gaagcgggcg accgggtact cctccatgaa gccgtagccg
     3421 ccgaacacct gggtggcgat gcgcgtcgcc gtcacggccg cttcggtgga gtagagcttg
     3481 gcgatcgacg ccgcctcctt gaactccttc gtcgagcgac cggcgtcctt gatcgccgcc
     3541 gccttgtacg tgaggccacg agccgcctcc gccatcacct tcaggtcggc gatctggaac
     3601 gccacaccct gcttgctgcc gatcggcacg tcgaacgtgg tgcgttcacc ggcgtactgc
     3661 acggacatgt caaggcaggc ctggatgcag ccgaccgcca gcgcagcgat cgcgacccgg
     3721 ccgtcgtcga gggtggcgag gaattgcgcg tagcccttgc cgcgctcgcc gagcaggttg
     3781 ctctccggca cgtgcacgtc gtcgaagctc agcgggtggg tgtcgctggc gtgccagccg
     3841 agcttgtcgt acgccggctc ggccgtgaag ccgggcgtgc ccgtcgggat gatgatggcc
     3901 gatacctcgg cacggtcctt cccagtggtc gggctcgtcg agacgccggt ccgggcggtc
     3961 acggtcacga ccgaggtgat gtcggagccg gagttcgtga tgaactgctt gctgccgttc
     4021 accacccact cgccgtcgac gaggtccgcc ttcgtcctgg tcgccccggc gtcggacccg
     4081 gaacccggct cggtcaaccc gaagccggca agcgtcgtac cggtcacgag gtcgggcagc
     4141 catgtctgct tctgctcgtc ggacccgtac gtcaggatcg gattgatgcc gagaccgaca
     4201 gccgcctcga gcgtgatgcc catcgactgg tcgacccggc cgatctcctc gatcgcgacg
     4261 ca
//
LOCUS       DATBFZ010000008         4228 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_1317246, whole genome shotgun sequence.
ACCESSION   DATBFZ010000008 DATBFZ010000000
VERSION     DATBFZ010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 4228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4228
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1317246"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            180..1028
                     /locus_tag="VH419_00175"
     CDS             180..1028
                     /locus_tag="VH419_00175"
                     /inference="COORDINATES: protein motif:HMM:NF016816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HEY7042061.1"
                     /translation="MKVFVSCDMEGVAGVVDWAQCRTDGGAAYELGCRLMLGEVNAAI
                     KGACEVGATDVVVNDSHGAMANLDPERLAAPASYISGRHKPGYMMAGLDETFDAVFFV
                     GYHGSISGESSVLSHTYNPDVISGVRLGGEYVGESGINLLVANGYRVPLALVTGDAQT
                     LAEVGKLAPDVVGVQVKSSLTRFAAHSLHPQDARARIGDGARTALERVRAGTIAPPSV
                     PEDPSLEVELQTADMAAVAAWLRGVERIGTRTVRVTGEGTAPESVAVYDAFVALTYIT
                     RQAGGR"
     gene            1025..2575
                     /locus_tag="VH419_00180"
     CDS             1025..2575
                     /locus_tag="VH419_00180"
                     /inference="COORDINATES: protein motif:HMM:NF005303.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEY7042062.1"
                     /translation="MTQTRAISGGAMTEHAVVIAGGGPTGLMLAGELSLAGIDVVIVE
                     RHTSQELDGSRSGGLHSRTIEVLDQRGIAERFLSAGQEHPGLGFAMIPLDISDFPTRH
                     NYLLALWQRDFERILAGWVEELGVPILRGCEMVGFAQHDTGVDVALSGDASVGASVGA
                     SVGAEYLVGCDGGRSLVRKVAGIDFPGLNPSASWIIAEVEMDEEPEVGVRTEGGGIGP
                     VNRAGAGGPYRVVLKERQVDDRTEPSLQDLREALIVAYGTDFGVHSPTWISRFTDMAR
                     QAASYRHGRVLLAGDAAHVHPPQGGQGLNIGVQDAVNLGWKLAQAVDKTSPDSLLDTY
                     HAERHPVGARVLHNTMAAVALGTPDDRHQALRETVAELLSMDEPRKRFAGMLSGLDVH
                     YDLGEGHPLLGRRMPDLDLRTVTGPTRVFTLLHEARPVLLNLGETGRFDIAAWADRVR
                     LVDAEHDGAWELPVLGEVAAPPAVLIRPDGHVAWAGDLTDPALPRALATWFGAATPDE
                     SGGSGSLR"
     gene            complement(2558..3004)
                     /locus_tag="VH419_00185"
     CDS             complement(2558..3004)
                     /locus_tag="VH419_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042063.1"
                     /translation="MTTPPEGPLGLRLSSLVRAGGTAATLLAGNPAGLLRTTLLAGGS
                     NVVAREIDPAGIQVLSISPDADINVGGEGGSRGGQADLITTASSTASWIAANLLLAKL
                     ASLLPLPRIVKAAVFGAGVYALDDMVVKKVTAVREHKPAEESAKAA"
     gene            3067..4104
                     /locus_tag="VH419_00190"
     CDS             3067..4104
                     /locus_tag="VH419_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY7042064.1"
                     /translation="MTPQIAVEARDLVKHFGDTVAVDGVSFAIPEGTVLSMLGPNGAG
                     KTTTVRMLTTLSVPTSGGGSVGGHDIVNDQHGVRRSMGLTGQAATVDELLTGRENIRL
                     IGRLYGIPRRRIRALADELLERFQLSDAADRTAKTYSGGMRRRLDLAVSLVANPPVLF
                     LDEPTTGLDPRSRVELWDVLRELVRGGTTLLLTTQYLEEADTLADEIIVIDRGRVIAA
                     GTPTELKDQAGRASVVLTVTHHSDLSRAAELVGRCSTELHVEEAQRRIVAPANGLEDM
                     TRIAHLLQEQSVPVDDLGLMRPSLDDVFLHLTGHRAEAEEDEAGDGHGEAGTSAGRDD
                     AETRRPEEALR"
BASE COUNT          683 a         1433 c         1486 g          626 t
ORIGIN      
        1 caccctagga cgcgacgagc caacgcgcgc agccgcgccg acgtaccggg caggcatcgt
       61 tgccaacccg ataccgacgc aggcggcggc acaccaccgt ggcgggtgtc cgggcgccgc
      121 cacgtggcag gatcgactga cgagccggca gcagtccaag ccggccgagg aggacgcggg
      181 tgaaggtgtt cgtctcgtgc gacatggagg gtgtcgcagg tgtggtggac tgggcgcagt
      241 gccggaccga cgggggagcg gcatacgagc tgggctgccg gctgatgctg ggcgaggtca
      301 acgcggcgat caagggggca tgcgaggtcg gcgcgaccga cgtcgtcgtc aacgactcac
      361 acggtgcgat ggcgaacctc gacccggaac ggctcgcggc accggcgtcg tacatcagcg
      421 gccgacacaa gcccggctac atgatggcgg gtctcgacga gacgttcgac gcagtgttct
      481 tcgtcgggta ccacgggtcg atctcggggg agtcgtcagt gctgtcgcac acctacaacc
      541 cggacgtcat cagcggcgtg cgactgggcg gcgagtacgt cggggagagc gggatcaacc
      601 tgctcgtggc caacggctac cgggtcccgc tggcgttggt gaccggtgac gcgcagaccc
      661 tcgcggaggt gggcaagctc gcaccggacg tcgtcggcgt acaggtgaag tcctcgctga
      721 cgaggtttgc tgcacactcg ctccatccac aggacgctcg cgcccggatc ggggacggcg
      781 cgcgtacggc gctcgaacgc gtccgcgcag gaacgatcgc gcctccttcc gtgccggagg
      841 acccgagcct cgaggtcgag ctgcagaccg ccgacatggc agccgtcgcg gcatggctga
      901 ggggcgtcga acggatcggc acgcgaaccg ttcgggtgac cggcgaagga acagcgccgg
      961 agagtgtcgc cgtgtacgac gcgttcgtcg cgctgacgta catcacccgg caggctggtg
     1021 gccggtgacg cagacgcgtg cgatctcggg aggggcgatg accgaacatg cggtggtgat
     1081 cgcgggcggc ggcccgaccg gtctcatgct tgcgggcgag ctgtcgttgg cggggatcga
     1141 cgtcgtcatc gtcgaacgac acaccagtca ggaactcgac ggatcgcgct ccgggggtct
     1201 gcactcccgc accatcgagg tgctcgatca gcgtggcatc gctgagcggt tcctctcggc
     1261 cgggcaagaa cacccgggcc ttggcttcgc catgatcccg ttggacatca gcgacttccc
     1321 cacccgccac aactacctgc tggcgctctg gcagcgggac ttcgaacgca tcctggcggg
     1381 ctgggtcgag gagctcggag ttccgatcct tcgtggctgc gagatggtgg gcttcgctca
     1441 gcacgacacc ggtgtcgacg tcgcgctgtc gggagacgcg tcggtcggcg cgtcggtcgg
     1501 cgcctcggtc ggcgcggagt acctggtcgg ctgcgacggc ggacgcagtc tggtccgcaa
     1561 agtggcaggc atcgactttc ccgggttgaa tccgtcggcc agctggatca tcgccgaggt
     1621 cgagatggac gaggagcctg aggtcggggt gcgcaccgaa ggtgggggca tcggtcccgt
     1681 caaccgcgcc ggggccggcg ggccgtaccg agtcgtgctg aaggaacggc aggtcgacga
     1741 ccgcaccgag ccgagcttgc aggacctccg cgaggcactg atcgtcgcgt acgggactga
     1801 cttcggggtg cacagcccga cctggatctc ccggttcacc gacatggcgc ggcaggcggc
     1861 gtcgtaccga cacggccgtg tgctcctcgc cggagacgcc gcgcatgtgc atccaccgca
     1921 gggcggccag ggcctcaaca tcggcgtaca ggatgccgtg aacctggggt ggaagctggc
     1981 ccaggcggtc gacaagacct ctcccgacag tctgctggac acctaccacg ccgaacggca
     2041 tccggtcggt gcccgggtgt tgcacaacac catggcggca gtggcgctcg gcactcccga
     2101 cgaccgtcac caggcccttc gcgagaccgt tgccgaactg ctgagcatgg acgagccccg
     2161 caagcgcttc gccgggatgc tctccgggct cgacgtccac tacgacctcg gtgaggggca
     2221 tccgctgctc gggcggcgca tgccagacct cgacctgcgt accgtcaccg gtcccacgcg
     2281 ggtcttcacc ctgctgcacg aggcccggcc ggtcctgctg aacctcggcg aaaccggccg
     2341 gttcgacatc gctgcttggg cggatcgagt ccggttggtc gacgccgagc acgacggtgc
     2401 gtgggagctt ccggtcctgg gcgaggtcgc tgcgccgccg gcagtgctga tccgacctga
     2461 cggacatgtc gcttgggctg gcgacctcac ggacccggcg cttcctcgcg cccttgccac
     2521 ctggttcgga gcggccacgc cggatgagtc cggcgggtca ggcagccttc gctgactcct
     2581 cggccggctt gtgctccctc acggcggtca ccttcttgac gaccatgtcg tcgagcgcgt
     2641 agacgcctgc accgaacacc gccgccttga cgatgcgagg cagcggaagc aggctggcca
     2701 gtttcgccaa cagcaggttg gccgcgatcc aggaggccgt ggacgacgcg gtcgtgatga
     2761 ggtccgcctg cccaccgcga ctgccgcctt caccgccgac gttgatgtcg gcgtcagggc
     2821 tgatcgacag gacctggatg cctgccgggt cgatctctct ggcaaccacg ttcgagccgc
     2881 ccgccagcag ggtggtccgg aggagaccgg ccggattccc ggccagcagg gtcgcggccg
     2941 tgccgccggc gcggacgagt gagctgagcc gcagcccgag cggcccttcg ggaggcgttg
     3001 tcatgcccgt caccgtaccg tgcaaatggt tggaggtctg tcgccgcctc gacctagggt
     3061 tgacgcatga ctccccagat cgcggtcgag gcacgggacc tggtcaagca cttcggagac
     3121 accgttgccg tcgacggcgt cagcttcgcc attcccgagg gcaccgtgct cagcatgctc
     3181 ggcccgaacg gcgccgggaa gaccacgacc gtccgcatgc tcacgacgct gagcgtgccc
     3241 accagtggcg ggggatcggt tggcgggcac gacatcgtca acgaccagca cggcgtacgc
     3301 cggagcatgg gtctcaccgg ccaggctgcc acggtcgacg agctcctcac cgggcgggag
     3361 aacatccggc tcatcggcag gctttacggc ataccccgac gtcgcatccg ggcactcgcc
     3421 gacgagctgc tcgagcgttt ccagctcagc gatgccgcgg accggacggc gaagacgtac
     3481 tccggcggca tgcggcggcg gctcgacctc gcggtgagcc tggtggccaa cccacccgtg
     3541 ctgttcctcg acgagcccac caccggcctc gatccgcgga gccgggtcga gctctgggac
     3601 gtgctgcgcg agctcgtgcg aggcggcacg accttgctgc tcaccacgca gtatctggag
     3661 gaggccgaca cgctggccga cgaaatcatc gtcatcgacc gtggtcgggt gatcgcagcc
     3721 ggcactccaa ccgagctcaa ggaccaggca ggtcgagcca gtgtggttct caccgtgacc
     3781 caccattccg acctctcgcg tgccgccgag ctcgtaggtc gctgctccac ggagctgcac
     3841 gtcgaggagg cgcaacgtcg catcgtcgcg cccgccaacg ggctcgagga catgacgcgc
     3901 atcgcgcacc tgcttcaaga acagagcgtc cccgtcgacg acctcgggct gatgaggccc
     3961 agcctcgacg acgtcttcct ccacctcacc ggtcaccggg ccgaggccga agaggacgag
     4021 gcgggggacg gccacggcga agcggggacg tccgccggac gcgacgacgc cgagacccga
     4081 cggcccgagg aggccctccg atgaccgccg tcaccgagcc cagccagacc ggtgggatct
     4141 cgagcctgga gctcgaacgc cgcaacgtcc tccagcagtc gatcaccgtg gtgtggcgaa
     4201 acctgatcca catcaagcgg atgcccga
//
LOCUS       DATBFZ010000009         5631 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_3286430, whole genome shotgun sequence.
ACCESSION   DATBFZ010000009 DATBFZ010000000
VERSION     DATBFZ010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 5631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5631
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3286430"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(23..403)
                     /locus_tag="VH419_00195"
     CDS             complement(23..403)
                     /locus_tag="VH419_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042065.1"
                     /translation="MTIEAYDPFDLPDWLGTHHVSWQATSGLHDQPVVRGRLTSDEGD
                     EIALDLLAVDAAYPRVACPEDLRKAAHQAWHFGQVELVVADGRVAAAVPGIGFDANLA
                     CDVLIRLAKSVGAPSDHYTVSLAL"
     gene            complement(620..1480)
                     /gene="tatC"
                     /locus_tag="VH419_00200"
     CDS             complement(620..1480)
                     /gene="tatC"
                     /locus_tag="VH419_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEY7042066.1"
                     /translation="MTTFFRLHGRSDSGGERDPAPVDEGRMTLTEHLRELRRRMFISI
                     VAIFVGFVVGWVFYDDIAKLLTDPYTTTVRQLAEDQGINSQAVISGVGAPFILQAKVA
                     LVSGLVLSSPIWLYQIWAFIMPGLHRNERKWTLIFVAIAGPLFAAGVVLGYVVLPKGL
                     SVLIGFTPEQVTNLVDLNSYLSFVLRILLVFGVAFEIPLFVVLLNLAGVVRGRHLARW
                     RAWIIFGTFVFAAVATPSTDPITMLLLAFPMVVLFLVSEVIAHLVDRRRGLHRPADYA
                     EYDDDEASPI"
     gene            complement(1519..1860)
                     /gene="tatA"
                     /locus_tag="VH419_00205"
     CDS             complement(1519..1860)
                     /gene="tatA"
                     /locus_tag="VH419_00205"
                     /inference="COORDINATES: protein motif:HMM:NF001854.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HEY7042067.1"
                     /translation="MFEATTTLDPNMAAFGGIGAPEVIIIVLVLLLLFGGRKLPEVAR
                     GTGRALRIFKAETKGLMDDDEDDKDVKRTDTTAQPPPQITQSDTRPKPADIETPSPNA
                     SDSLPPTHTEK"
     gene            complement(2520..3488)
                     /locus_tag="VH419_00210"
     CDS             complement(2520..3488)
                     /locus_tag="VH419_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009078184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HEY7042068.1"
                     /translation="MSASAHEQVERMLALVPYLRSRDGISVDEVARDFGVPPSQIVKD
                     LKVLWYCGLPNSVTGDMIDIDMEALDGDGIVKLSNADYLTRPLRLAPHEALAMMVALR
                     ALRETSGSSQRETVDRTLAKLEAATGESAAPASAVDIHVDQHDDAIRASVDQALATQR
                     RLHLRYYVPGRDETTERDADPIRLVFSEGHGYLEAYCYRAGEVRLFRIDRITAAEVLD
                     QPAEPPEGLGLHDLSKGLFQPDPEDALAVLDLQPAARWVSEYYPIEREQELGDGRLRV
                     ELRFSDERWLQRLVMRLGGAASVVQPESVASAVRDRARAALAQYSA"
     gene            complement(3485..4474)
                     /locus_tag="VH419_00215"
     CDS             complement(3485..4474)
                     /locus_tag="VH419_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007077767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HEY7042069.1"
                     /translation="MTARKAERLMNLTICLLVARTFIPKDRIRQAVEGYHDLSDEAFD
                     RMFDRDKEELRVLGVPIELGTIDKAFEDEVGYRISRARFELPEISLEADEAAVVGLAA
                     RVWQHASLASATTQGLRKLRAGGVDVDESTLAILEPQLATDEPAFDDIFAAVTSQTPI
                     AFEHRRSGEAGSTRRHLEPWGIVSWHGHWYVVGHDRDRDAPRMFRVSRIEGGVESVGE
                     PGSYSPPPDLNLRALASSLAPPHPHSSARVLVRHGAGDSLRRRAASSKPAQEGWTEIE
                     LPYGSGSSLAEELTSYGPDVCVLGPAEVRAAVVRRLSALAEAEQPAEPAGQPR"
     gene            complement(4597..5526)
                     /locus_tag="VH419_00220"
     CDS             complement(4597..5526)
                     /locus_tag="VH419_00220"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEY7042070.1"
                     /translation="MRRTLAAALLPLLLVLTGCGDNPEEGVTAKKATIADIQVTGEPG
                     KKPVVDFKPPVTFATTQSKVLDKGPGKGDEIAENSEVTVNYLGINAADGSEFGSTYDK
                     GDQPAIFTVNQVIPGFGKGLQGAQAGDRVLITVTPEDAYGESGNGSDIGKNTSVIFVV
                     DVLKVKNPAKPVTLTEKDIPKLELDKDGNPTKFVTTPGIPDSVSKLGVAVLKEGKGAK
                     VTSNDTLTVNYLGQIYPDGKVFDESYSTGQPATFPLSNVIAGWQQGLVGQREGSRVVL
                     VIPSELGYGATGQGKDIPPDSDLIFVVDIEKIG"
BASE COUNT          900 a         1894 c         1898 g          939 t
ORIGIN      
        1 cgcgccgccc gtcgtgcggc cttcagagtg cgagcgacac tgtgtagtgg tcactcggcg
       61 cgcccacgga cttcgccagc cggatcagca cgtcgcaggc caggttcgcg tcgaaaccga
      121 tgcccggcac ggcggcggcc acccgtccgt ccgccacgac gagctcgacc tgcccgaagt
      181 gccaggcctg gtgagccgct ttgcgaaggt cctcgggaca cgcgacgcga gggtacgccg
      241 cgtcgaccgc caacaggtcc agcgcgatct cgtccccctc atcagacgtc agcctgccac
      301 ggacgaccgg ctggtcgtgc agaccacttg tggcctgcca gctgacgtga tgcgtaccga
      361 gccaatcggg caggtcgaac ggatcatagg cctcgatggt catctccccg agaatcgctt
      421 cgcgtgccgc acgaccagaa gtggctcacg tgtcggctgg tgtgccgcgt cggaagttct
      481 ttcacaggtg cggcgagaac aggtggatgc atcgcaaggc ggaggaaggg aggcgatgcg
      541 cagcatcgtt gaccgacgac aacgccgcga ggcgccgcct ggaccggcgc aacggggaca
      601 agagttccga cgcgaggcac tagatcggcg aggcctcgtc gtcgtcgtac tcagcgtagt
      661 cggcgggacg gtgcagcccg cggcgtcggt cgaccagatg ggcgatgacc tcggagacga
      721 ggaacagcac caccatcggg aacgccagga gcagcatcgt gatcggatcc gtcgacgggg
      781 tggccacggc agcgaacacg aacgtgccga agatgatcca cgcccgccat cgggcgagat
      841 gacgaccgcg tacgacgccg gcaaggttca gcagcaccac gaacagtggg atctcgaacg
      901 ccacgccgaa gaccaggagg atcctgagca cgaagctgag gtagctgttg aggtcgacca
      961 ggttggtcac ctgttcaggt gtgaacccga tcagtaccga caggcccttg ggaagcacga
     1021 cgtagcccag cacgacgccg gcggcgaaca acggtccggc gatggccacg aagatcagcg
     1081 tccacttgcg ctcgttgcga tggagcccgg gcatgatgaa cgcccagatc tggtagagcc
     1141 agatcggact cgagagcacc agtcccgaga cgagcgcgac cttcgcctgc aggatgaacg
     1201 gtgcgccgac gccggagatg acggcctgcg agttgatgcc ctggtcttcc gccagctggc
     1261 gcaccgtcgt cgtgtagggg tcggtcagca gtttggcgat gtcgtcgtag aacacccagc
     1321 cgacgacgaa gccgacgaag atcgcgacga tcgagatgaa catccggcgg cgaagctcgc
     1381 gcagatgttc ggtgagggtc atccgcccct cgtcgaccgg ggcggggtca cgctcgccgc
     1441 cggagtccga ccgcccgtgc aaacggaaga acgtggtcac gggaaccgcc tcgtcctagc
     1501 gcgccggcgg gatcgccgtc acttctcggt gtgcgtgggc ggaagcgagt cgctcgcgtt
     1561 cggcgacggc gtctcgatgt cggccggctt cggcctggtg tcgctctggg tgatctgcgg
     1621 cggcggttgg gctgtggtgt cggtgcgctt gacgtccttg tcatcctcgt cgtcgtccat
     1681 caggcccttg gtctcggcct tgaagatccg cagtgcccgg ccggtgccgc gagccacctc
     1741 gggaagcttg cggccgccga agagcagcag caagacgagc acgatgatga tgacctcggg
     1801 cgcgccgatg ccaccgaacg cggccatgtt ggggtccagg gtcgttgtcg cctcgaacat
     1861 ttgtgcctct tcgtcccgat agtgtcacag ccgcaacgag cggctgttgc cgtcagtcat
     1921 cctacgctct ggattcggca cgcccactcg agcggtcgga ctctcatggc agattctttc
     1981 gtttctcccg cgaccccgac agcgggatgg tcgaggtgtt tacggtggac cacctcgctg
     2041 gcacggcaga cctgcggact cctccggttt cggtgggcct ccttccggaa agccacctcc
     2101 cgcacgaatc ggacagacgt cgggcggtgg tttgcctggt tagccaggca gatctgcctt
     2161 gggaagggtg gatttgcctg ggtagccatg caaaccgccc tcggcgaccg acggatgtcc
     2221 gaaaaccgcc agtcacacac cgaccacgag ggacggaagg ccggccgacc ggctcggcag
     2281 gcgcgaacgt gtcccgctcc tcgttgcaga tccacctgag ctcatgacaa cagaagccgg
     2341 gcgaccgccc tcaccgatca cgaccgtctc cgccccgaca gaaacctgag gagtctccag
     2401 atctgcctcg ctacctgtcc acgcggatgg ctacccgggc aaaccaccac cccagtctgt
     2461 tcgaccacga gctgttcgaa atggtgacga acgagatcgg gagcggccga tggcctgcgt
     2521 cacgcggagt actgcgccag tgctgcgcgt gcacgatcgc gcaccgccga ggccaccgac
     2581 tccggttgaa cgacggacgc cgcaccgccg aggcgcatca ccagccgctg gagccagcgc
     2641 tcgtcgctga agcgcagctc gacgcgcagc cgtccgtccc cgagctcttg ctcccgctcg
     2701 atcgggtagt actccgacac ccaccgggcc gccggttgca ggtcgaggac cgccagcgca
     2761 tcctccggat ctggctggaa caaccccttg gagaggtcgt gcaggcccag tccttccggt
     2821 ggttcggccg gctggtcgag gacttcggca gcggtgatcc ggtcgatacg gaacagccgc
     2881 acttcgcccg cccgatagca gtaggcctcg aggtacccgt gcccctccga gaagaccagc
     2941 cgaatcgggt cggcgtcacg ctctgtggtc tcgtcgcggc cgggtacgta gtagcgcagg
     3001 tggagtcgac gctgggtcgc cagcgcctgg tcgacgctgg cccggatcgc gtcgtcgtgc
     3061 tggtccacgt ggatgtcgac cgccgacgcg ggggctgccg actcgccggt cgccgcctcg
     3121 agcttggcca gcgtccggtc gacggtctcc cgctgactgc tcccgctggt ctcgcggagg
     3181 gcgcgcagcg ccaccatcat ggccaacgcc tcgtgtgggg cgagtcgcag cggtcgggtg
     3241 aggtagtcgg cattgctcag cttgacgatg ccgtcgccgt cgagcgcctc catgtcgatg
     3301 tcgatcatgt cgccggtgac cgagttgggg agcccgcagt accacaggac cttgaggtcc
     3361 ttcacgatct gggacggcgg tacgccgaag tcacgggcga cctcgtcgac ggagatcccg
     3421 tcgcggctcc ggaggtacgg aacaagcgca agcatccgct cgacctgctc gtgagcgctg
     3481 gcgctcatcg cggctgtcct gcgggctcgg ccggctgttc ggcctcggcg agggcgctca
     3541 accgtcgtac gacggcagcc cgcacctccg ccggtccgag gacacagacg tccggaccgt
     3601 acgacgtcag ctcctccgcc agcgacgaac cactgccgta cggcagctcg atctccgtcc
     3661 agccttcctg cgccggcttg gacgaggccg cccggcggcg caggctgtct ccggccccgt
     3721 gcctgaccag cacacgggcg gagctgtgcg ggtgcggggg cgccagactg gacgcgagcg
     3781 cccgcaggtt caggtccggt ggcgggctgt acgagccggg ctcgccgacc gactcgacgc
     3841 ctccctcgat ccgggagacc cggaacatgc gcggggcgtc ccggtcgcgg tcgtggccga
     3901 cgacgtacca gtgtccgtgc caggagacga tgccccatgg ttcgaggtga cgccgcgtgc
     3961 tgccggcctc ccccgatcgg cggtgctcga aggcgatcgg cgtctgggag gtcacggccg
     4021 cgaagatgtc gtcgaaggcc ggctcgtccg tcgcgagctg cggctcgagg atcgccagcg
     4081 tcgactcgtc gacgtcgacg ccgccggccc gcagcttgcg caggccctgg gtggtcgccg
     4141 aggcgaggct ggcatgctgc cagaccctcg cagcgaggcc gaccaccgct gcctcgtccg
     4201 cctcgaggct gatctcgggc agctcgaagc gggcccggct gatccggtag ccgacctcgt
     4261 cctcgaacgc cttgtcgatg gtgccgagct cgatcgggac accgaggacc cgcagctcct
     4321 ccttgtcccg gtcgaacatc cggtcgaacg cctcgtcgga gaggtcgtgg tagccctcga
     4381 cggcctgcct gatcctgtcc ttcgggatga acgtccgcgc cacgagcagg cagatcgtga
     4441 ggttcatcag ccgctcggcc ttgcgtgcgg tcaccagacg tcacctcttt ctgcgggcct
     4501 cgtcgtcgta ctcgccatct gctggtcgtg gcctgccggg tcttccctct tcacggtagt
     4561 gaagccgact gcccctcgcc cggagtggtg gcagcgtcag ccgatcttct cgatgtcgac
     4621 gacgaagatc aggtcggagt ccggcgggat gtccttgccc tgaccggttg cgccgtatcc
     4681 cagctcgctc gggatcacga gcacgacccg ggagccttcc cgttgcccga cgaggccctg
     4741 ctgccacccg gcgatgacgt tgctgagcgg gaacgtcgcg ggttgacccg tgctgtagga
     4801 ctcgtcgaac accttcccgt cgggatagat ctggccgagg tagttcacgg tcagcgtgtc
     4861 gttcgaggtg accttcgcgc ccttgccctc cttcagcacg gccaccccga gcttggagac
     4921 cgagtccgga atgcccggtg tcgtcacgaa cttcgtgggg ttcccgtcct tgtcgagctc
     4981 gagcttcggg atgtccttct cggtgagggt gaccggcttg gccgggttct tcaccttcag
     5041 gacgtcgacg acgaagatca cgctggtgtt cttgccgatg tcgctgccgt tgccggactc
     5101 gccgtacgcg tcctccgggg tgaccgtgat gagcacccgg tcaccggcct gagcgccctg
     5161 cagacccttg ccgaagcccg ggatcacctg gttgacggtg aagatggcgg gctggtcacc
     5221 cttgtcgtac gtcgacccga actccgaccc gtcggcggcg ttgatcccga ggtagttgac
     5281 ggtgacttcg gagttctcgg cgatctcgtc gcccttgccc ggtcccttgt ccagcacctt
     5341 gctctgcgtg gtggcgaacg tgaccggcgg tttgaagtcg acgacgggct tcttgcccgg
     5401 ctcccctgtg acctggatgt cggcgatcgt cgccttcttg gcggtgacac cctcctcggg
     5461 gttgtcgccg cagccggtca ggaccaggag cagcggcagc agggcagcgg cgagggtacg
     5521 acgcacgggg aaacacctca cggatcgggc accgggacga ccggggacag gactcacatc
     5581 aagacgaacg acgggacagc ctaactcagg gtcacatcga ctcgatcagc c
//
LOCUS       DATBFZ010000010         8419 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_6237938, whole genome shotgun sequence.
ACCESSION   DATBFZ010000010 DATBFZ010000000
VERSION     DATBFZ010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 8419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8419
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6237938"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="VH419_00225"
     CDS             <1..546
                     /locus_tag="VH419_00225"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF033740.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarP family serine protease"
                     /protein_id="HEY7042071.1"
                     /translation="SLEGTGFVYRPGRVMTNAHVVAGVDEPTVRIGETDYSGTVVSYD
                     PAVDVAVIDVPDLPAPALDFASREARSGAPAAVLGFPENGPFDAQPARIRDRRTLRSP
                     DIYGDDTVFRDTYSIRAVVRQGNSGGPLVDARGDVLGVIFAASVTDGDTAYALTARQV
                     SEAALAGASASAGVDTGDCAL"
     gene            complement(601..999)
                     /locus_tag="VH419_00230"
     CDS             complement(601..999)
                     /locus_tag="VH419_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011753839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEY7042072.1"
                     /translation="MTTPHGPDAEEPTIGKLVADTSRDLSALVRGEIELAKTELKFSV
                     RAGGIAIGLFVAAAFLVLLAIVMISIAFAYFLHWLYFGLGTSYLIVFVVYVLIAALLG
                     FIGSRKMKQVKAPEQTMAAVKSNKQVLKRT"
     gene            complement(1096..2565)
                     /locus_tag="VH419_00235"
     CDS             complement(1096..2565)
                     /locus_tag="VH419_00235"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEY7042073.1"
                     /translation="MAELETFGAIVLVAALGLVVAILSNRVSERLHVPAPAIFFGLAV
                     LAAAVYPDLAELPLVGTEEIVTVAVAAILFDGGMHIGLNRLRESLGVIAATGVLGTFL
                     TAGAVALVVHTLFGFDWWVAVLLGTAIAPTDPAVVFSVLGRREIGGRAGTILEGESGA
                     NDPVGIALLVSLLAAGGVSLGAWGDVAGTFALQMIVGTAIGIIGGLATLWFMRNVPLP
                     SEALYPLRALASGFVVYGLATVLHGSGFLAIFVAGIVLGDERAPYKGEVERFTSAIAS
                     LSEVVAFVALGLTVDVDVLVDSDVWLTGILIGAIVTFVVRPIVVGAVLLPFGLGRNER
                     LFVLWSGLKGAVPLLLGILVLDEQVPDAERVYGIVIVVVIMSVLLQGTLVPTAASLLS
                     IPMRMREPEPWALGVRLRHEPNGVHRLEVAARSVAAGTRIEDLPISSDDVWVSFVVRR
                     RRLVTVRGDTVLEPGDEALILADPALHDELVSMFTTASR"
     gene            complement(2650..4623)
                     /gene="acs"
                     /locus_tag="VH419_00240"
     CDS             complement(2650..4623)
                     /gene="acs"
                     /locus_tag="VH419_00240"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017933284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HEY7042074.1"
                     /translation="MSDTPSETLSNLLHEERRFEPPAELAAQANATDADYEAAEKDRL
                     GFWAKQAERISWAEPFDEVLDWSNPPFAKWFVGGKLNAAYNCVDRHVENGLGDKVAYH
                     WVGEPGDTRDITYAELKDEVCKAANALLDLGVQTGDRVAIYMPMIPETVISMLACARI
                     GAPHTVVFGGFSADALKGRIQDCDAKAVITADGGYRRGAPSALKPAVDQALGECPGVS
                     AVLVVRRTEQDVEMTDGRDHWWHDVVEKQSTEHQPEAFDSEHPLYVMYTSGTTGKPKG
                     ILHTTGGYLVGTSYTHWAIFDLKPETDIYWCAADIGWVTGHSYIVYGPLANAATSLMY
                     EGTPDTPHKGRWWEIIADHKATILYCAPTAIRTFMKWGADIPAKFDMSSLRVIGSVGE
                     PINPEAYIWYRKSIGGDRAPVVDTWWQTETGMIMISPLPGVTAGKPGSAMKAIPGIEA
                     LVVDDSGNAVKPGSGGYLVLTEPWPAMLRTLWGDDERYKETYWSRFPELYFAGDGAKL
                     DEDGDIWLLGRVDDVMNVSGHRLSTTEIESALVSHPKVAEAAVVGANDEMTGQAPVAF
                     VILRESAGDGGDGVVAELRDHVAKEIGAIAKPKSIMVVPELPKTRSGKIMRRLLRDVA
                     ENRPTGDVTTLADSSVMDLIKENLASGKAADED"
     gene            complement(4782..5399)
                     /locus_tag="VH419_00245"
     CDS             complement(4782..5399)
                     /locus_tag="VH419_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017933283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HEY7042075.1"
                     /translation="MTSQRIEQSAPTSPVPPASPIADPAPLGLAAFALTTFVLSCVNS
                     GIIPDSTEPVVFGLALAYGGLAQFAAGIWEFAKGNTFGATAFCSYGMFWVSFWYLTGH
                     TDLTGMSADDAAKGIGTYLLAWAIFTGYMSVAATRVSGAVLVVFVLLTLTYLVLAIGE
                     YATSDGWHHLGGWLGLITALAAWYASFAGVTSFTFKRPLVPTAPR"
     gene            complement(5605..7119)
                     /locus_tag="VH419_00250"
     CDS             complement(5605..7119)
                     /locus_tag="VH419_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007079410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HEY7042076.1"
                     /translation="MNAAAGVLGVTVVSIATVAIGAYGLRFSRTTSDFYVASRSVGPT
                     LNASAISGEYLSAASFLGVAGLVLAHGTDMLWYPVGWTAGYLVLLVLVAAPLRRSGAY
                     TLPDFADFRLDSRRVRHTSSALVVLIGILYLMPQFQGAGITLRAITGAPKPVGAVVVA
                     VIVLVNVLSGGMRSITFVQAFQYWLKLTALLVPAAIIFRTWAGNGSPSALDADEWIQP
                     MTGNHSVYVTYSLILATFLGTMGLPHVVVRFYTNPDGRAARRTTVVVLGLLSLFYLLP
                     PIYGFLGRVYAPDLVAAHRTDSVVLELPSHVFTSPLGDMLTALVSAGAFAAFLSTSSG
                     LTVSVAGVISQELVGSHVDGVRAFRIGTVVATVISLTFALASTGLAVANAVELAFAVA
                     ASTFCPLLLLGIWWRGLTAYGAGLGLVVGGALSGGAVLATLFGYTAHGWYGVLLSQPA
                     AWTVPASFAAMIIGSLATRHRQLPHIGQVMVRLHTPEHVDVDRGTFHPEMPRPR"
     gene            complement(7116..7478)
                     /locus_tag="VH419_00255"
     CDS             complement(7116..7478)
                     /locus_tag="VH419_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020389040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042077.1"
                     /translation="MPSRVTVTSPRTSAPHRRRTPATQEIDDQTVLGEIYMRSLIRAQ
                     LRLGAVVLLVLAVTLGGLPLLFAQVPAVRDAHIVGIPLPWLILGVLVYPVLLGLGWYY
                     VRQSERNERDFSELVDRR"
     gene            complement(7509..8303)
                     /locus_tag="VH419_00260"
     CDS             complement(7509..8303)
                     /locus_tag="VH419_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008356300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEY7042078.1"
                     /translation="MPDPARSEERARAGLHVLVVDDEHPVLDELGYLLAHDDRVGTVS
                     TAASGGAALKQLEREDIDAVFLDIAMPGLSGLDLARLLTRFKEPPSIVFVTAHDAHAV
                     EAFELNALDYLLKPVRQERVAEAVRRIVETRDPRSQADEMIPVELAGVIRFIRRRDVQ
                     YAEAQGDYVRLHTADGSHLLRTPLGALEERWASAGFVRIHRSLLVAVAQITEVRFEHG
                     RGSVRVGDAVLQVSRRHTAQLRDMLLHPAASETADTQRPVARAPSR"
BASE COUNT         1394 a         2819 c         2929 g         1277 t
ORIGIN      
        1 agcctcgagg gcaccggctt cgtctatcgg cccggacggg tcatgacgaa cgcccacgtc
       61 gtcgccgggg tcgacgagcc cacggtccgt atcggggaga cggactactc gggcacggtc
      121 gtctcctacg acccagccgt cgacgtcgcc gtgatcgacg tacccgacct gccggcgccg
      181 gcgctcgact tcgcctcgcg ggaggcccgt tccggcgcac ctgctgccgt cctcggcttc
      241 ccggagaacg gtccgttcga cgcgcaaccc gcacgcatcc gcgaccgacg gaccctgcgc
      301 agccccgaca tctacggcga cgacacggtg ttccgggaca cctactcgat ccgggcggtc
      361 gtccggcagg gcaactccgg tggcccgctc gtggacgccc gcggtgacgt cctgggcgtg
      421 atcttcgccg cgtcggtgac cgacggcgac accgcctatg ccctcaccgc caggcaggtg
      481 tccgaggccg ccctggctgg ggcgtcggca tccgcgggcg tcgacaccgg tgactgcgct
      541 ctgtgagccg gcagccggtg agccagttga gcccatctcg tcgcggccgt gacgtcggcg
      601 tcaggtgcgc ttgaggacct gcttgttgct cttgacggcg gccatcgtct gttcgggtgc
      661 cttgacctgc ttcatcttgc gactgccgat gaagccgagc agtgcggcga tcagcacata
      721 gacgacgaag acgatgaggt acgacgtgcc gagcccgaag tagagccagt gcaggaagta
      781 cgcgaacgcg atcgagatca tcacgatcgc gagcagcacg aggaaggcgg cggcgacgaa
      841 cagaccgatc gcgatcccgc cggcgcggac gctgaacttg agctcggtct tcgccagctc
      901 gatctcgcca cgtacgagcg cggacaggtc gcgactggta tcggcgacaa gcttgccgat
      961 cgtcggttct tcggcgtcag gaccgtgagg ggtcgtcatc ggatgtcctc tcgtcgcgtg
     1021 ccgtgcgcta gcgaaaccct acgggttcgc cctggtccgc gcgattcgtg gtggccgcat
     1081 ggcgctgtcg tggcttcagc gcgacgccgt ggtgaacatc gagacgagct catcgtgcag
     1141 ggcggggtcg gccaggatca gcgcctcgtc gcccggctcg agcaccgtgt cgccgcgcac
     1201 ggtgaccagc cgtcggcggc gtacgacgaa gctcacccat acgtcgtcgg acgagatcgg
     1261 cagatcctcg atccgtgtcc cggcggccac cgagcgtgcg gcgacctcga gccggtgcac
     1321 gccgttgggc tcatgccgaa gccggacgcc cagcgcccac ggttcgggtt cgcgcatacg
     1381 catcgggatg ctgagcaggg aggcggccgt cgggaccaac gttccctgaa ggaggacgga
     1441 catgatcacg accacgatca cgatgccgta gacgcgttcg gcgtctggta cctgctcatc
     1501 gagcaccaag atcccgagca agagcggaac ggcgcccttg aggccgctcc agagcacgaa
     1561 gagccgttcg tttcgaccca gaccgaaggg cagcagcacc gcgccgacga cgatcggtcg
     1621 gaccacgaag gtgacgatcg cgccgatgag gattccggtg agccagacgt cgctgtcgac
     1681 cagcacgtcc acgtcgacgg tgagaccgag agcaacgaac gccacgacct ctgacagcga
     1741 ggcgatcgcg gacgtgaagc gttcgacctc acccttgtac ggcgctcgct cgtcacccag
     1801 gacgatcccg gcgacgaaga tggcgaggaa ccccgagccg tgcagcacgg tggccaggcc
     1861 gtagacgacg aacccggagg cgagtgcgcg cagtgggtag agcgcctcgc tcggaagcgg
     1921 cacgttgcgc atgaaccaca gcgtcgccag cccgccgatg atgccgatcg ccgtaccgac
     1981 gatcatctgg agagcgaacg taccggcgac gtcgccccag gcaccgaggc tgacgccgcc
     2041 cgcggcgagc aggctgacga gcaacgcgat acccaccggg tcgttcgcgc cggactcgcc
     2101 ctccaggatc gtcccggccc gccctccgat ctcgcgtctg cccagcaccg agaagacaac
     2161 tgccgggtcg gtcggcgcaa tggccgttcc gagcaacacc gcgacccacc agtcgaagcc
     2221 gaacagggtg tgcacgacga gcgcgacggc tcctgccgtc aggaacgtgc cgagcacgcc
     2281 ggtcgcggcg atcactccga gtgactcccg gagtcggttg agcccgatgt gcatgccgcc
     2341 gtcgaagagg atggccgcga cggccaccgt gacgatctct tcggtgccga ccagcggcag
     2401 ctccgcgaga tcggggtaga cggcggccgc gagcaccgcg aggccgaaga agatcgccgg
     2461 cgcaggaacg tgcaggcgtt cgctgacacg gttggagagg atggcgacga ccagacccag
     2521 cgcggcaacc aggacgatcg ccccgaaggt ctcgagctcg gccaccagac ctcccggacg
     2581 tcgtcggcat ctgtgccgac cagacttccc ggctcgccct acgctcctcc tgcgaggcta
     2641 gcagccagtt cagtcctcgt ccgcggcctt gcccgaggcc aggttctcct tgatcaggtc
     2701 catcaccgac gagtcggcca gcgtggtcac gtcgccggtg ggacggttct cggcgacatc
     2761 acggagcagc cggcgcatga tcttccccga ccgggtcttc ggcagctcgg gcacgaccat
     2821 gatcgacttc ggcttggcga tcgcaccgat ctccttcgcg acgtggtcgc gcagctcggc
     2881 aacgacgccg tcgccgccgt ctccggcgct ctcgcgcaag atcacgaacg cgaccggcgc
     2941 ctgaccggtc atctcgtcgt tggcgcccac gaccgcggct tccgcgacct ttggatgcga
     3001 cacgagcgcg gactcgatct cggtggtcga caaccggtga ccggaaacgt tcatcacgtc
     3061 gtcgacgcgg ccgagcagcc agatgtcgcc gtcctcgtcg agcttggctc cgtcaccggc
     3121 gaagtacagc tccgggaacc gcgaccagta cgtctccttg taccgctcgt cgtcacccca
     3181 caacgtgcgc agcatcgcgg gccaaggctc ggtcagcacg agatagccgc cggatcccgg
     3241 cttcactgcg ttgccggagt cgtcgacgac gagcgcctcg atgccgggga tcgccttcat
     3301 cgccgagccg ggcttgccgg ccgtgacacc cggaagcgga ctgatcatga tcatgccggt
     3361 ctcggtctgc caccacgtgt cgacgaccgg agcccggtcg ccgccgatcg acttgcgata
     3421 ccagatgtac gcctcggggt tgatcggctc gccgaccgag ccgatgacgc gcaacgagga
     3481 catgtcgaac ttcgcgggga tgtcggcgcc ccacttcatg aacgtccgga tcgcggtcgg
     3541 ggcgcagtac aggatcgtcg ccttgtggtc ggcgatgatc tcccaccagc ggcccttgtg
     3601 cggagtgtcc ggggtgcctt cgtacatcag cgaggtcgcc gcgttggcga gtggcccgta
     3661 gacgatgtag gagtggccgg tgacccagcc gatgtcggcg gcgcaccagt agatgtccgt
     3721 ctccggtttc aggtcgaaga tcgcccaatg cgtgtacgac gtaccgacga ggtagccgcc
     3781 ggtcgtgtgc aggatgccct tcggcttccc ggtggtcccg gacgtgtaca tgacgtacag
     3841 cgggtgctct gagtcgaacg cttcgggctg gtgctcggtc gactgcttct cgacgacgtc
     3901 atgccaccag tggtcgcgcc cgtcggtcat ctcgacgtcc tgctcggtcc ggcgtacgac
     3961 gagcacggcg ctgacgccag ggcattcccc gagggcttgg tcgacggcag gcttcagcgc
     4021 ggacggggcg ccacggcgat agccgccgtc ggccgtgatg actgctttcg cgtcacagtc
     4081 ctggatccgg cccttgagcg cgtcggccga gaagccgccg aacacgacgg tgtgcggagc
     4141 gccgatgcgc gcgcaggcga gcatcgagat gacggtctcg gggatcatcg gcatgtagat
     4201 cgcgacccgg tccccggtct gcacgccgag gtcgagcagc gcgttggcgg ccttgcacac
     4261 ctcgtccttc agctcggcgt acgtgatgtc ccgggtgtcg ccgggctcgc cgacccagtg
     4321 gtacgcgacc ttgtcgccca ggccgttctc gacgtgccgg tccacgcagt tgtacgccgc
     4381 attgagcttg ccgccgacga accacttcgc gaacggcggg ttgctccagt cgagcacctc
     4441 gtcgaacggc tcggcccagc tgatgcgctc cgcctgcttg gcccagaacc cgagccggtc
     4501 cttctccgcc gcctcgtagt ccgcgtccgt cgcgttggcc tgcgcggcca gttccgcggg
     4561 tggctcgaag cgccgctcct cgtgcagcag gttggagagg gtctccgatg gggtgtcact
     4621 catgttctct cctcgaacga tgccggtggg ttcagcggcg gtccgccgtg gaactacgag
     4681 agtggcggtg tgccagcacg gtcacaccgc cactccacca ggctggtggc cgtagggcaa
     4741 gcgatgccgt cagctgccgt cagctgccgt cagcgctgcg ttcaacgcgg cgcggtcggg
     4801 accagcggtc gtttgaacgt gaacgacgtg acgccggcga acgacgcgta ccaggccgcc
     4861 agggcggtga tgaggccgag ccatccgccg aggtggtgcc agccgtctga cgtcgcgtac
     4921 tcaccgatgg cgagcaccag gtacgtcagc gtcagcagca cgaagacaac gagcacggca
     4981 ccgctgaccc gggtcgccgc cactgacatg tagccggtga agatcgccca ggcgagcagg
     5041 taggtgccga tgcccttggc ggcgtcgtcg gcgctcatgc cggtcaggtc ggtgtgtccg
     5101 gtcaggtacc agaacgacac ccagaacatg ccgtacgagc agaaggcggt ggccccgaac
     5161 gtgttgccct tggcgaactc ccagatgccg gcggcgaact gggcgaggcc gccgtacgcc
     5221 agcgcgagcc cgaacaccac gggttccgtg ctgtcgggga tgatccccga gttcacgcaa
     5281 ctgagcacga acgtcgtcag tgcgaaagct gccaggccga ggggtgccgg gtcggcgatg
     5341 ggggacgccg gtggaaccgg gctggtgggt gcggactgct cgatccgttg cgaggtcatg
     5401 atgactcctt gcacgtcgca atctgtccgg caggcaccgg acgcgttcac gctaggtgtg
     5461 atgcgcgcca cgatgaaggg gtcggcgacc ggcgttcggc ggtcggccgc gatgggtcgc
     5521 caggcggtcg acggcgtacg acgaacggtc gttccagcgc gacgagcggt acgtcgcaaa
     5581 cccgcgccct gaccggcgga ggattcaacg tggtctcggc atctccggat ggaacgtccc
     5641 tcggtcgacg tcgacgtgct ctggggtgtg cagccggacc atcacctgtc cgatgtgcgg
     5701 cagctgccgg tgtcgggtgg ccagcgaccc gatgatcatc gctgcgaagc ttgccgggac
     5761 ggtccacgcg gccggttggc tgagcaggac gccgtaccag ccgtgcgccg tgtagccgaa
     5821 caaggtggcg agcacggccc ctccggacag cgcgccgccc acgacgagac ccaacccggc
     5881 gccgtaggcg gtgagaccgc gccaccagat gcccaacagc agcagcgggc agaacgtgga
     5941 cgcggcgacg gcgaaggcga gctcgacggc gttcgccacc gccaggcccg tcgacgccag
     6001 cgcgaacgtc agcgagatca ccgtggccac gacggtgccg atccggaacg cgcggacgcc
     6061 gtcgacgtga ctgccgacga gctcttggct gatgaccccg gcgaccgaga cggtcagccc
     6121 agaggaggtg gacaggaacg ctgcgaacgc gccggcactc accagggcgg tcagcatgtc
     6181 tccgagcggg ctcgtgaaga cgtggctggg cagttcgagc acgaccgagt cggtgcggtg
     6241 ggccgcgacg aggtcggggg catagacgcg gccgaggaag ccgtagatcg gcggcagcag
     6301 gtagaagagg ctcagcagcc ccaggaccac gacggtcgta cgccgggcgg cccgcccgtc
     6361 ggggttggtg tagaagcgca cgacgacgtg ggggagcccc atggttccca ggaacgtggc
     6421 caggatcagc gagtaggtga cgtacacgct gtggttgccg gtcatcggct ggatccactc
     6481 gtcggcgtcg agtgcggacg gtgaaccgtt cccggcccag gtgcggaaga tgatcgcagc
     6541 ggggaccagc aacgcggtca gcttgagcca gtactggaag gcctgcacga aggtgatgct
     6601 gcgcatccct ccggacagca cgttgacgag cacgatgacc gccacgacga cggcgccgac
     6661 cggcttcgga gcgccggtga tcgctcggag cgtgatgccg gcgccttgga actgtggcat
     6721 caggtacaag atgccgatca gcacgacgag cgcactcgac gtgtgcctga cccgtctcga
     6781 gtcgagccgg aagtcggcga agtccggcag ggtgtacgcg ccggaccggc gcagcggcgc
     6841 ggccaccagg acgagcagga cgaggtagcc ggcggtccat cccacggggt accagagcat
     6901 gtccgtcccg tgcgcgagga ccagtccggc cacgccgagg aaggacgccg ccgacaggta
     6961 ctcgccgctg atggcggaag cgttgagcgt cggtccgaca gagcgggatg cgacgtagaa
     7021 gtcgctcgtc gtacgagaga acctgagccc gtacgcgccg atcgcgaccg tggcgatcga
     7081 cacgaccgtg accccgagga ctccggcggc ggcgttcacc gccggtcgac cagctcggaa
     7141 aagtcccgct cgttgcgctc ggactgccgg acgtagtacc agccgagtcc gagcagcacc
     7201 ggatacacga gtacgccgag gatcagccag ggcaacggga tcccgacgat gtgcgcatcg
     7261 cgtacggccg ggacttgagc gaacagcagg ggcaggccac ccagtgtcac cgcgaggacc
     7321 agcagcacga ccgctccgag ccggagctgg gcacggatca gcgaccgcat gtagatctcg
     7381 ccgagaaccg tctggtcgtc gatctcctgt gttgccggcg tgcgacgtcg gtggggtgcc
     7441 gatgtccgtg ggctcgtcac cgtcacgcgc gacggcacgg gctcatcgtg ggtcgtcatc
     7501 tcatgtgttc atcgagaagg ggcgcgagcg accggccgct gggtgtcggc tgtctctgac
     7561 gcagcggggt gcagcagcat gtcgcgcagc tgcgcggtgt gtcgccggct cacctgcagg
     7621 acggcgtccc ccaccctgac ggagccgcgg ccgtgttcga acctgacctc ggtgatctgg
     7681 gccaccgcga cgagcaggct ccgatggatc cgtacgaagc ccgcgctggc ccaccgctcc
     7741 tcgagcgctc cgagcggcgt acgcagcaag tgcgaaccgt cggctgtgtg gagccgcacg
     7801 tagtcgccct gcgcttcggc gtactggacg tcccgtcgcc tgatgaaccg gatcactccg
     7861 gccagctcga ccgggatcat ctcgtccgcc tgcgatcggg gatcgcgggt ctcgacgatc
     7921 cggcggactg cctcggccac ccgctcctgg cggaccggct tgagcaggta gtcgagcgcg
     7981 ttgagctcga aggcctcgac ggcgtgggca tcgtgggcgg tgacgaacac gatgctcggt
     8041 ggctctttga accgggtgag cagccgggcc aggtcgagac ctgagagccc cggcatggcg
     8101 atgtcgagga agaccgcgtc gatgtcttct cgctcgagct gcttgagcgc cgcacctccc
     8161 gatgcggccg tcgatacggt gccgactcgg tcgtcgtgtg ccagcagata gccgagctcg
     8221 tcgaggaccg ggtgctcgtc gtcgacgacg agcacatgca gtcccgcccg ggcacgctcc
     8281 tcagacctgg cagggtccgg catggcgccg acgatagcgc ggctttggcc gtaccgccct
     8341 ccgagcaggc gaccggcgtg ttgcctgccg gaacggctgt tgacctcagg cgccgtggtg
     8401 gtcttcgacg gtgaaggtg
//
LOCUS       DATBFZ010000011         9107 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_5253684, whole genome shotgun sequence.
ACCESSION   DATBFZ010000011 DATBFZ010000000
VERSION     DATBFZ010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 9107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9107
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5253684"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..703)
                     /locus_tag="VH419_00265"
     CDS             complement(<1..703)
                     /locus_tag="VH419_00265"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEY7042079.1"
                     /translation="MTDAAGSSVPREEVMTEEDAAEQERDRARFDLLPDGVVVADADG
                     IVTVANATARRLLELGDAEPIGRHVSHVLELQDLAGVDWFDCVRPYDGLAIRRRLQEA
                     VWLTPSGHEVLVTGLLLRDRPGGKVVTSAWGIRDARSRQRVDRERSDLVATVAHELRS
                     PLTGVKGFTATLLSKWERFTESQKQLMLRTVDADADRLTRLIGELLDVARIDTGRLSV
                     RKEPVDLVEALGRQLE"
     gene            complement(784..1623)
                     /locus_tag="VH419_00270"
     CDS             complement(784..1623)
                     /locus_tag="VH419_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020577505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEY7042080.1"
                     /translation="MRLTDTGSGPALTPRSARVKEARKLVRRSGRAAARRFLAEGPQA
                     VGEALRYDSPAGPCVVEVFATDPAADRHPRLRSAARGLGVDWQVVDDHALASLTGTVT
                     PQGVVAVCRFLDADLDAVLADTPRLVVICANVRDPGNAGTVIRCADAVGASAVVLAGE
                     SVDPYNGKAVRASVGSLFHLPIVTGLPVETAVAAVRAAGLVVLAADGAGDVSVDDLLD
                     RGELGRRTAWIFGNEAWGLDAATTALADRVVRVPIYGRAESLNLATAAAVCLYASARA
                     QRA"
     gene            complement(1614..2006)
                     /gene="rplT"
                     /locus_tag="VH419_00275"
     CDS             complement(1614..2006)
                     /gene="rplT"
                     /locus_tag="VH419_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEY7042081.1"
                     /translation="MARVKRAINAHKKRRETLEKASGYRGQRSRLYRKAKEQVQHSLV
                     YSYRDRRARKGEFRRLWIQRINAGARANGMTYNRFIQGLREAGVEVDRKILADLAVND
                     TAAFAALVEVAKKAVPVQPAVESAKASA"
     gene            complement(2085..2288)
                     /locus_tag="VH419_00280"
     CDS             complement(2085..2288)
                     /locus_tag="VH419_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007028043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bL35 family ribosomal protein"
                     /protein_id="HEY7042082.1"
                     /translation="MKRHSGMSKRIKATGSGKLVRLRARRKAGAAFASTPIRGSRKNR
                     RRHAGTVEVASSDTKQVKRLLGR"
     gene            complement(2384..3049)
                     /gene="infC"
                     /locus_tag="VH419_00285"
     CDS             complement(2384..3049)
                     /gene="infC"
                     /locus_tag="VH419_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002548324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEY7042083.1"
                     /translation="MRVNDRIRVPEVRLVGPNGEQVGIVRIDDALRLAQEADLDLVEV
                     APTARPPVCKLMDYGKFKYENAQKARETRRNQTNTVIKEMKLRPKIDAHDYETKKGHV
                     VRFLKQGDKVKITIMFRGREQHRPELGFRLLQRLAGDVEELGYVESAPKQDGRNMIMV
                     IGPHKKKSEARAEAKAERDERAALRAADRDAERAARIERSAAAKTAPKKTPRRSENLD
                     PDM"
     gene            3473..4072
                     /locus_tag="VH419_00290"
     CDS             3473..4072
                     /locus_tag="VH419_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose transferase"
                     /protein_id="HEY7042084.1"
                     /translation="MDISDGGEPPGGLPAAAASKVVSRLLSEPPRAGRTRVVAVDGPA
                     GSGKTVLAEALRRDAQARGLEVVVVHMDDLYAGWTGLDDELGARVVDQILHPLANGEP
                     VRWQRFDWYAERFDGWETAPPPDALVLDGCGSGARAHAPYVALLVWLEADEPSRVRRG
                     IARDGEQVLPHWLAWMALEQQHFAVNDTRARADLRFTTD"
     gene            complement(4059..4610)
                     /locus_tag="VH419_00295"
     CDS             complement(4059..4610)
                     /locus_tag="VH419_00295"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEY7042085.1"
                     /translation="MTGIGHVTLPAGTFELRPPHVAEAEHAMAMLRDPDVRQWNPGPD
                     EVTLDVVRDWLVRSADWSSGEFAVWSVHDAQRYVGNALICRIDPDQREAWVAYRTAPW
                     ARGRGAATSAVQAMTSFAFEVLGLERLRLPHAVANAASCRVADKAGYVYEGTERGGYR
                     DPAGVRWDSHVHARLRDGVVSRS"
     gene            complement(4607..4831)
                     /locus_tag="VH419_00300"
     CDS             complement(4607..4831)
                     /locus_tag="VH419_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042086.1"
                     /translation="MRDEAGWFRSTFQHDADAFVEAGLWLRSPLRRYVLALATAGAVL
                     PRSLSRAHAGGGRTPRRSDEPAATGQTPPR"
     gene            complement(4849..6771)
                     /locus_tag="VH419_00305"
     CDS             complement(4849..6771)
                     /locus_tag="VH419_00305"
                     /inference="COORDINATES: protein motif:HMM:NF012239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation factor GTPase family protein"
                     /protein_id="HEY7042087.1"
                     /translation="MPTLNLGIVAHVDAGKTTLTERLLYDAGVISRVGSVDHGDTVTD
                     SDAIERRRGITIRSAVVTFDIGDRKVNLIDTPGHSDFVAEVERALSVLDGAVLVVSAV
                     EGVQAQTRVLIRILERLQIPFLVFVNKVDRVGASQEETLGALREALAGDAVAMNLVTG
                     IGTRSATVCTRPSLELRRDLELRLAEYDDDVLRHFVDATEPLAMPHALNLLTRTSAAG
                     HLHPVYFGSAMTGAGVEELRAGIARHLPAYDADVDAPLHGRVFKIERRRSGRAAFVRI
                     LAGRLCVRDNVPVFHRTRDGGVVERSGRVTAIRVFARGQETDEVPAAAGDIAVVTGLD
                     DVAIGDQLGRWNADVADRLFAPPTLEAVVEPAVPAGRAALFEALQRLTDQDPFIQTRL
                     DGVDEEVTVSLYGEVQKEVLTARLSEEFGVEARFLPTQVIHVERVSGVGEARGRVPMG
                     NVAVGLRVEPGPVGCGVDYRLGIERGWLLPSFHVAVEEGVFLGLQDGLFGWQVVDCRV
                     TLVDGRFCAPTPSAGEFRRLTTSLLRKALERAGTTVCEPYSEFDLEFPSHVASTVLGA
                     LAAGRASDLQPQVGRERGQVTGVLPTASVPGLEQKLPDITEGRGVWFTRPAGHRPVVG
                     EPVRRRHVLIPPTRGT"
     gene            7371..8699
                     /locus_tag="VH419_00310"
     CDS             7371..8699
                     /locus_tag="VH419_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEY7042088.1"
                     /translation="MAEKRSGEGAEEKPIRVPPALGSGIDEDVARIIERAQAEGLALT
                     GEGGLLPEMIKRAVEAAMQAELTDHLGYERYAVEGRGSGNSRNGTTSSTVHTNAGPVG
                     LDKPRDRNGEFDSVVVPKGTRRLGEFDEMILSLYAKGMTDRDIAEHLQATYGAEISHE
                     TVANITDAINEQVKEWRNRPLDEVYPILYVDAIRLRIRDGGQVRVKSCHLAIGVDLEG
                     RKRVLGMWIEHTEGAKFWAGVLTELRNRGIKDVLIVCCDGLTGLPEAIRAIWPHATVQ
                     TCVVHLIRNCMRYASWKDRKAIAAMLRPIYTAPTLEAAEQALEAFAGSDIGNRCPAAV
                     DAWRRAWEDFIPFLELPQPIRQVVYTTNAIESYNYQLRKVTKNRGQFPSDDAAYKLLY
                     LAICDIEVRTTSRGGNRDKKLLQRGAASQHWTEALNHFALIYPGRLPEPA"
     gene            complement(8846..>9107)
                     /locus_tag="VH419_00315"
     CDS             complement(8846..>9107)
                     /locus_tag="VH419_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042089.1"
                     /translation="GQRGQDLPVGGREQVGDVVLERADVLEQDAVASKVPGQSRCSLL
                     SVVWRRQTSPPPVGPVLRVGSVSSLLCKWLEAVMLRLCCVAW"
BASE COUNT         1430 a         3127 c         3140 g         1410 t
ORIGIN      
        1 gctcgagctg acgcccgagc gcctcgacca gatcgaccgg ctccttgcgg accgagagtc
       61 gcccggtgtc gatccgtgcc acgtcgagca gctccccgat cagccgggtg agccggtcgg
      121 catccgcgtc gacggtgcgc agcatcagct gcttctgtga ctcggtgaac cgctcccact
      181 tcgacagcag ggtcgcggtg aaacctttga cgcccgtgag gggtgaccgc agctcgtgcg
      241 ccaccgtcgc caccaggtcc gaccgctcac ggtcgacacg ctggcgcgag cgcgcgtccc
      301 gaattcccca ggccgaggtg acgaccttgc cgccgggccg gtcccgcagg agcagccctg
      361 tcacgagcac ctcatggccg gacggggtga gccacaccgc ctcctggagt cggcggcgaa
      421 tggccaggcc gtcgtacggc cgtacacagt cgaaccagtc gacgccggcg agatcctgca
      481 gctcgagaac gtgggagacg tgccggccga tcggctcggc gtcaccgagc tcgagcagcc
      541 gccgcgcggt ggcgttggcg acggtgacga tcccgtcggc atcagcgacc accacgccgt
      601 cggggaggag gtcgaagcgt gccctgtccc gctcctgctc ggcggcgtcc tcctccgtca
      661 tgacctcttc tcgagggacg ctcgaaccgg ctgcgtcggt catccgagcc cctcctgtcc
      721 ggtccgagca gagcaggtgt gtcacagact agtacgacgg gcgggcgtcg tcgcgggcca
      781 tcgtcatgcc cgctgagccc gggcagatgc gtacaaacag accgcggcgg cggtcgcgag
      841 gttgaggctc tcggcgcggc cgtagatcgg gacccgcacc acccggtcgg ccagtgccgt
      901 ggtggcggcg tccagtcccc acgcctcgtt cccgaagatc cacgcggtac gccggccgag
      961 ctctccccgg tcgaggaggt cgtcgacact cacgtcaccg gccccgtcgg cggccagcac
     1021 cacgagcccg gcggcccgta ccgcagcgac cgccgtctcg accggcaggc cagtgacgat
     1081 cgggaggtgg aacaacgaac cgacgctggc acgcaccgcc ttgccgttgt aggggtcgac
     1141 cgactccccg gccagcacga cggcggacgc accgacggcg tcagcgcagc ggatgacggt
     1201 gccggcgttt ccggggtcgc ggacgttcgc gcagatgacg acaagccgtg gcgtgtcggc
     1261 gagcaccgcg tcgaggtcgg cgtccaggaa ccggcacacg gcgaccacgc cttgcggggt
     1321 caccgtgccg gtcagggacg cgagagcatg gtcatcgacg acctgccagt cgacgccgag
     1381 cccgcgggcg gccgacctga gccgtgggtg ccggtccgcc gcggggtcgg tggcgaagac
     1441 ctcgacgacg cacgggccgg cgggtgagtc gtaccgcaag gcttcgccga cggcctgggg
     1501 gccctcggcg aggaatcgtc gggcggcggc gcgaccactg cgccgtacga gcttgcgggc
     1561 ctccttgacg cgggccgacc gtggcgtcag cgcaggccct gaacctgtgt ccgtcaggcg
     1621 gatgccttcg ctgactcgac ggccggctgg accggaacgg ccttcttcgc gacctcgacg
     1681 agagccgcaa aggcagccgt gtcgttgacg gccaggtcgg caaggatctt gcggtcgacc
     1741 tccacgccgg cttcacgcag accctggatg aaacggttgt aggtcatgcc gttggcgcgc
     1801 gcaccggcgt tgatgcgctg gatccacaac cggcggaact cccccttgcg cgcgcggcgg
     1861 tcacggtagc tgtacaccag cgagtgttgg acctgctcct tggccttgcg gtagagccgg
     1921 gaccgctgac cgcggtagcc ggaggccttc tcgagggtct ccctgcgctt cttatgggcg
     1981 ttgatcgccc gcttgacgcg tgccacgtgt tactccttgg aagcttcgtg cgcctgctgt
     2041 cgcaggcatg tctgatcgtg gtgggtcgag ggggtcttgg ccagctacct gccgagcagc
     2101 cgcttgacct gcttggtgtc gctgctcgcg acctccacgg tgccggcgtg ccgccgacgg
     2161 ttcttcctcg atccccggat gggtgtggac gcgaacgcgg cgcccgcctt gcggcgtgcc
     2221 cgcagccgga cgagcttgcc cgagcccgtc gccttgatgc gcttggacat gcctgagtgg
     2281 cgcttcatct tcggcatggt gccgatctcc gtctctttga gctgatgtgc tgactgctgg
     2341 ttcggtcggt ggtgctgggt ggtgtgtgac gcgcagagcg ctgtcacatg tccggatcga
     2401 ggttctccga acggcgcggc gtcttcttcg gtgctgtctt tgccgccgcg gagcgctcga
     2461 tccgggccgc tcgctcagcg tcccggtcgg ccgcccgcag ggcagctcgc tcgtcgcgct
     2521 cagccttggc ctcggctctg gcctcggact tcttcttgtg cgggccgatc accatgatca
     2581 tgttgcggcc gtcctgcttc ggggccgact cgacgtaccc cagctcctcg acgtcgccgg
     2641 ccagtcgctg cagcaaccgg aagccgagct cagggcggtg ctgctcgcgc ccgcggaaca
     2701 tgatcgtgat cttgaccttg tcgccctgct tcaggaagcg cacgacgtga cccttcttgg
     2761 tctcgtagtc gtgtgcgtcg atcttcgggc gaagcttcat ctccttgatg accgtgttgg
     2821 tctggttgcg ccgcgtctcg cgtgccttct gggcgttctc gtacttgaac ttgccgtagt
     2881 ccatgagctt gcacaccggc gggcgagccg tcggggcaac ctcgaccagg tcgagatcgg
     2941 cctcctgcgc caggcggaga gcatcgtcga tgcggacgat gccgacctgc tcaccgttcg
     3001 gtccgacgag gcgtacctca gggacgcgga tgcggtcgtt gacacgcaac tctgtgctga
     3061 tgagtcctcc tgttgatggt ctgtcacgga tgtggcgcct gggccggaca aacaaaaatg
     3121 gcctccgtgc cacgcacgaa agccaccacg tctgccgccg agtcggcgaa acggcgagcc
     3181 accacggacg gttcgcgtcc tgcggatgtc tcgcgacctg acccgacgac ctcgcggtcg
     3241 gtgcgggtgg gaggtggcct ccgcttgtac acctgttcga acaccgagcc gaagcccggc
     3301 attccaccac aggtgggtca gcgccacagg ttaccagtcg gccaccccct ccgtcacttc
     3361 cgccgacccg ccgctggtgt tctccgcgaa atgcgggaaa gtgcaggtcg acacgcccat
     3421 acggagatgc acttcaccgc aattcgcgga aaccagcggg gaggagaacg ccgtggacat
     3481 cagcgacggc ggcgaaccgc ccggcgggtt gcctgctgct gccgcctcga aggtcgtcag
     3541 ccggctgctg tcagagccgc cgcgagccgg ccggacgcgg gtcgtcgccg tcgacgggcc
     3601 ggccggatcg ggcaagaccg tgctggcgga ggcgctgcgc cgcgacgcgc aggcgcgagg
     3661 actcgaggtc gtcgtcgtgc acatggacga cctgtacgcc ggctggactg gtctcgatga
     3721 cgagctcgga gcccgggtcg tggaccagat cctgcacccg ctggcgaacg gtgagccggt
     3781 ccgctggcag cgcttcgact ggtacgcgga acgcttcgac ggatgggaga ccgcaccgcc
     3841 tcccgacgcg ttggtcctcg acggctgcgg atcaggcgca cgtgcgcacg cgccgtacgt
     3901 cgcgctgctg gtgtggctgg aggcggacga gccgtcgcgg gtccggcgtg gcatcgcgcg
     3961 ggacggtgag caggtgctgc cccactggct cgcctggatg gcgctggagc agcagcactt
     4021 cgcggtgaac gacactcggg cgcgcgccga cctacgcttc acgaccgact gacgacgccg
     4081 tcgcgcagcc gggcgtggac gtgactgtcc cagcggacgc cagccgggtc gcggtagcca
     4141 ccccgctcgg tgccctcgta cacgtacccg gccttgtccg ccactcgaca ggaggcggcg
     4201 ttcgcgaccg cgtgcggcag ccgcagccgc tcgaggccga ggacctcgaa cgcgaagctc
     4261 gtcatcgcct gcaccgcgga ggtggcggca cctcgcccac gcgcccacgg ggccgttcgg
     4321 taggcgaccc acgcctcgcg ctggtcgggg tcgatccggc agatcaacgc gttgccgacg
     4381 taccgctgcg cgtcgtggac ggaccagacc gcgaactcgc ctgacgacca gtcggcgctt
     4441 cgcaccagcc agtcgcgtac gacgtcgagc gtgacctcgt cggggccggg attccactgc
     4501 cggacgtccg ggtcacgcag catcgccatc gcgtgctcgg cctcggcgac gtggggcggg
     4561 cgcagctcga aggtccctgc gggcaacgtg acgtgaccga tgcccgtcat cgcggaggtg
     4621 tctgcccagt cgcggcgggt tcgtcagacc ggcggggcgt ccggccgccg ccggcatgcg
     4681 cccttgacag cgagcgcggc agcacagcgc cagcggtggc cagcgccagg acgtaccgcc
     4741 gcagcggcga ccgcagccag agccccgcct cgacgaacgc gtcggcgtcg tgctggaagg
     4801 tggagcggaa ccagcccgcc tcgtccctca cgacgcgagc gtattgcgtc atgtcccccg
     4861 ggtgggcgga atcaggacat gccgacgacg taccggctcc ccgacaacgg gtcgatgacc
     4921 agcgggccgg gtgaaccaca ctccgcggcc ctccgtgatg tcgggcagct tctgctcgag
     4981 cccggggacc gacgcggtcg gaaggacgcc ggtcacctgg ccacgttcgc ggccgacctg
     5041 aggctgcagg tccgacgccc ggccagccgc cagcgctccc aacacggtgc tcgcgacgtg
     5101 cgacgggaac tcgagatcga actcgctgta cggttcgcag accgtggtgc ctgctcgctc
     5161 cagcgccttg cggagcaggc tggtcgtgag ccgccggaac tcaccggccg acggtgtcgg
     5221 agcacagaag cgcccgtcga cgagcgtcac ccggcagtcc accacctgcc atccgaacaa
     5281 gccgtcctgg agcccgagga acaccccttc ctcgacggcg acgtggaacg acggcagcag
     5341 ccagccgcgt tcgatcccca gccgatagtc gacaccgcag ccgaccgggc cgggctcgac
     5401 acgtagcccg accgccacgt tccccatcgg cacccgcccg cgggcctcac ccacgcctga
     5461 cacacgctcc acgtggatga cctgcgtggg caggaaccgc gcttcgaccc cgaactcctc
     5521 gctcagccgg gcggtcagaa cctccttctg cacctccccg tacaggctga cggtgacctc
     5581 ctcgtccact ccgtcgagcc gcgtctggat gaacgggtcc tggtcggtga gccgttggag
     5641 tgcctcgaac agcgcggcac ggcctgccgg tacggccggt tcgaccaccg cttccagcgt
     5701 cggcggcgcg aagagtcggt ctgccacatc ggcattccag cggcccagct ggtcgccgat
     5761 ggcaacgtcg tcgaggccgg tcacgaccgc gatgtcgcca gcagcggcgg gcacctcgtc
     5821 ggtctcctga ccgcgcgcga agacgcggat cgcggtcacg cggccggacc gctcgacgac
     5881 gccaccgtcg cgcgtccgat ggaacaccgg cacgttgtcc ctgacgcaga gccttcccgc
     5941 caggatccgc acgaacgcag cccttccgct acgacggcgc tcgatcttga agacccggcc
     6001 gtgcagcgga gcatcgacgt ccgcgtcgta cgccggcagg tgacgggcga tgccggcccg
     6061 cagctcctcg acacccgcgc cggtcatggc ggacccgaag tacacaggat ggagatgtcc
     6121 ggcggcgctc gtcctcgtca agaggttgag tgcgtgtggc atggcgagtg gctcggtggc
     6181 gtccacgaag tgccgcagga cgtcatcgtc gtactccgcc agccggagct cgaggtcgcg
     6241 gcgtagctcc agcgaggggc gcgtgcagac cgtcgccgag cgcgtgccga tgccggtgac
     6301 gaggttcatc gcgacggcgt ccccggcgag tgcctcgcgc agtgccccca gcgtctcctc
     6361 ctgcgacgcg ccgacccggt cgaccttgtt gacgaagacc aggaacggga tctgcaggcg
     6421 ctcgaggatc cggatgagga cgcgcgtctg agcctggaca ccctccacag ccgacacgac
     6481 caggacggcc ccgtcgagca cgctgagcgc gcgttcgacc tcggcgacga agtcggagtg
     6541 gccgggggtg tcgatcaggt tgaccttgcg gtcgccgatg tcgaacgtga cgacggccga
     6601 ccggatcgtg atgccgcgtc ggcgctcgat ggcgtcggag tcggtcacgg tgtcgccgtg
     6661 gtcgacgctg ccgacccggc tgatgacgcc tgcgtcgtac agcaggcgtt cggtcagtgt
     6721 ggtcttaccg gcgtcgacat gggcgacgat cccgagattg agggtgggca cgggtgacgg
     6781 ctgccttctg gtcgctcgga tgggtgaagg tgatctgtcc gagcgagcgg cgcatcccgt
     6841 gctccttcct tcgtagccct cacggcgact gtcgccgtct ccgcgtgacg gtagacctca
     6901 gctcgctccc ggcgccacag cttttcgggc ccggcgtggt ttgcctgggt agccatccga
     6961 atgcacggct ccccaggcaa accggggttc tccctgcggt ttctgtgggc cggcggcggg
     7021 tgaggtttgc gatgtttaca gcccaaaagg cgtgtcaaca tagcaaaact ccgatgggca
     7081 cagtgggttt gccgtctgaa catcgccaac ttcacgccgg ccacccgcgg agtccgaaac
     7141 cccccagcca cccaggccgg tccgatacgt gcggtaggtc gcttttccat ctcggggttt
     7201 gcctgggtag ccatccaagc ggacaggtag ccaggcaggt ctgccttggg aggggtggac
     7261 ttgcctgggt agccatgcaa accgcgggtg ggcgaccaac ggatgtccca agagggggtg
     7321 tcagatggtc tgtgtaaacg gctggcctga ttcgagagga gactcaggtg atggccgaga
     7381 agaggtctgg cgagggagct gaggagaagc cgattcgggt gccgccggcg ttggggtccg
     7441 ggatcgacga ggacgtcgct cggatcatcg agagggcgca ggccgagggg ttggcgttga
     7501 ccggggaggg cggtctgttg ccggagatga tcaagcgggc ggtagaggcg gcgatgcagg
     7561 ccgagttgac cgaccacttg gggtatgagc ggtatgcggt cgagggtcgt ggcagcggga
     7621 actcccgcaa cggcacgacc tcctcgacgg tgcataccaa cgccggcccg gtggggttgg
     7681 acaagccgag agaccgcaac ggcgagttcg actcggtggt ggtgccaaag ggcacccgcc
     7741 gactcggcga gttcgacgag atgatcttga gcctgtacgc caaggggatg accgatcgag
     7801 acatcgccga gcacctgcag gccacctatg gtgcggagat ctcgcacgag acggtcgcga
     7861 acatcaccga cgcgatcaac gagcaggtca aggagtggcg gaaccggccg ctggatgagg
     7921 tctatccgat tctgtacgtc gacgcgatcc ggctacggat ccgcgacggc ggccaggttc
     7981 gggtcaagtc ctgccacctg gccatcgggg tggacctgga gggccgcaaa cgggtgctcg
     8041 ggatgtggat cgagcacacc gagggcgcca agttctgggc cggtgtgctc accgaactgc
     8101 gcaaccgcgg gatcaaggac gtcctgatcg tgtgctgcga cggtctgacc gggctgcccg
     8161 aggcgatccg ggcgatctgg ccgcacgcga cggtgcagac ctgcgtggtg cacctgatcc
     8221 gcaactgcat gcggtatgcc tcttggaagg accgcaaagc gatcgcggcg atgctgcggc
     8281 cgatctacac cgccccgacg ctggaagccg ccgagcaggc gctggaggca ttcgccggct
     8341 cagacatcgg caaccggtgc ccggccgcgg tcgacgcctg gcgacgggcc tgggaggact
     8401 tcatcccgtt cctggagctg ccgcagccga tccgccaagt ggtctacacc accaacgcga
     8461 tcgagtccta taactaccag ctccgcaagg tgaccaagaa tcgtggccag ttcccctccg
     8521 acgacgccgc ctacaagctg ttgtatctgg cgatctgcga catcgaggtg cgcaccacca
     8581 gccgtggcgg gaaccgggac aagaagctgc tacaacgcgg cgccgccagc caacactgga
     8641 ccgaagcgct caaccatttc gcactcatct accccggccg actgcccgaa ccggcctgac
     8701 cggccgaccc gatgactgcc accactgcgt tccctgacag ccagcccacc cgtggcgacg
     8761 cgagccgggg tcaaagtgga gcgcccgcag cgcagcgagg acgaacgaca ttgacgccga
     8821 cgagcagagc acgacaatca gctggctacc aggcaacgca gcacaaccga agcatcacag
     8881 cctccagcca tttacacagc aaacttgaca cgctcccgac gcgcagtact gggccgaccg
     8941 gtggcgggga tgtctgccgc cgccacacga cgctgagcaa cgagcaccgc gactggccag
     9001 ggaccttgct cgctaccgcg tcctgctcca ggacatcagc gcgctcgagg acgacatcgc
     9061 cgacctgctc gcgcccaccg acgggcagat cctgaccacg ctgcccg
//
LOCUS       DATBFZ010000012         7970 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_3288490, whole genome shotgun sequence.
ACCESSION   DATBFZ010000012 DATBFZ010000000
VERSION     DATBFZ010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 7970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7970
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3288490"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="VH419_00320"
     CDS             <1..336
                     /locus_tag="VH419_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017934341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEY7042090.1"
                     /translation="DDLARGLGRRTGVDRLVLGAAIVLLAGGATALAGPIAFVGLVVP
                     HGVRAVVGTDYRSLLPLSAGYAAALTVVADTVGRVVLPPTEVQVGIMTAVVGVPVFLW
                     LVRRGRTGL"
     gene            336..1385
                     /locus_tag="VH419_00325"
     CDS             336..1385
                     /locus_tag="VH419_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008360354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEY7042091.1"
                     /translation="MTLLDVAPTRRPDRALELSRRARRGPVRRQRLVICGLVLALLLA
                     FAARVLLGDFTVTIPDFVRILFGADIPGASYIVMETKLPRAVLGVLVGASFGIGGAIF
                     QTTLRNPLASPDIIGVSVGASAASVFTIVVLGSSESSVSFAAIIGAVLVAVTVRVVAG
                     PVRGYRLVLVGVGVAAALQSVIQYFFTRADVYDAQAALRWLTGSLSAADWPGITILAV
                     FLLLCLPIIVWLARSLPVIELGPDAAASLGVTTRRTDALLMLAVVLTAVGVAAAGPVA
                     FVSFLAGPIARGLNGGRTTLVGAALTGAVIVVGADYVADYLLSDVNFPVGVITGAFGA
                     PYLLWLLARRERSQR"
     gene            1382..2233
                     /locus_tag="VH419_00330"
     CDS             1382..2233
                     /locus_tag="VH419_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020574998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY7042092.1"
                     /translation="MTLVEDDVVHVRTQQQPAARLRAENVTLGYDEHPVVESLSLEFP
                     DRRVTAVIGANACGKSTLLRGLGRLLRPQAGSVLLDGAAIHRLPTREVARTLGLLPQD
                     PVTPDGVTVVELVERGRHPHRNAFRRRSGADTEAVAAALELTDTAGLAGRVMDELSGG
                     QRQRVWIAMALAQETDLLLLDEPTTYLDLAHQLEMLDLLRELNAVRGTTIVMVLHDLN
                     LAARYADHLVAMRTGRVVAAGTPAEVVTEQNVNQVFDLDCRVISDPVAGTPLVVPVAR
                     RGQELSE"
     gene            2230..3183
                     /locus_tag="VH419_00335"
     CDS             2230..3183
                     /locus_tag="VH419_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017934344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055072 - obsolete iron ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-interacting protein"
                     /protein_id="HEY7042093.1"
                     /translation="MTATATDVHRPAPMIVDQVRVVSVERLSPSFARIELGGPALTEF
                     GVDGPLYDQRVKLIFPGPSGLLPRIDPYDDGWYAGWRRLPTEVRGHMRTYTVRTTRGR
                     GAGTTLVVDFVLHDEAGVTGPGAAWAAGAQVGDSLVVVAPRRGWPFGGIEFAPFPSSR
                     RLMLVADETAVPAVGGILDGLPSGLVGTAYLEVPVSADILDMHAPPGIDVVWLPRDGA
                     ELGTRLRRAVSAQFGVATVAAGPHEVDPDLWETPTYSSSGEPIDVRPPTTGAGGDLYA
                     WVAGESAVVTGLRRQLVRELGLDRRQVAFMGYWRRGVAMQA"
     gene            complement(3202..3903)
                     /locus_tag="VH419_00340"
     CDS             complement(3202..3903)
                     /locus_tag="VH419_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020387574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY7042094.1"
                     /translation="MELTHQFTVPAPVDVAWKAFNDLERIAPCFPGASLTSYDGDAFE
                     GICKVKLGPISLQYAGSGRFVERDEATHRAVIEAKGRDKRGNGTAAANVTTTLAASGD
                     DATDVTVTTDLSITGRPAQFGRGVMQDVSDKLLGQFAACLETKLGGEESTASAATAEP
                     QSTVPEPESAAAESAATPIGAEAAAAVSASSAEPTEELDLGATVMPVLVKRYAPYVIG
                     GLIALWLLKRLLRRS"
     gene            complement(3972..4826)
                     /locus_tag="VH419_00345"
     CDS             complement(3972..4826)
                     /locus_tag="VH419_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009074305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEY7042095.1"
                     /translation="MIPAAFDYTAPTTVADAITALGGDAEEIKVLAGGQSLIPVLKLR
                     LAAPTLLVDIGHIDELKGISEDGDAIVIGAMTTHDEVATNELVRDHAHVLAEAAQSVA
                     DPQVRHMGTLGGALVHSDPAGDLPAPILALDAELVIAGPGGERTVAAADFFEDLFTTV
                     VGEDEILTKIRIPKHTGWGGHYEKFTRVAQQWSIVAVAAAVKAENGQISAAKIGLTNM
                     GATALRATAVEQALVGQSATADAVKAACAQAAEGTNPPSDTNGDADYRRHLASVLTGR
                     AVLAAAGS"
     gene            complement(4823..7291)
                     /locus_tag="VH419_00350"
     CDS             complement(4823..7291)
                     /locus_tag="VH419_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008360870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEY7042096.1"
                     /translation="MTVTEERPTQEVGTARRRKEDARLITGRTRWTDNIKLPGMLHLA
                     MVRSPFAHARITSVNADEAKRAPGVVAVYTGADLADEQGGLPNAWMLTPEQVSPNHPS
                     IAVDRVAFAGEVVGIVVARSAAEARDAAELVEVDYEALPVALDLKAAAANEVLAHPDL
                     GTNISAVRTFDSAEAGTGGDVDDAIAKARENGIVIEREFRQQRLIPAFMEPRSVVVDP
                     TGEQMTVWTSTQVPHFVKIFISLVLGIPESKIRVIAPDVGGGFGGKLQFTPEEVLTVI
                     ASRHTGKPCKYTETRSESLMVAHHGRDQWQTLTLAADKDGTVTGFKVDLLCDMGAYLG
                     LVTSAIPFLGALMFNGIYKFPAYRWTGTNVFTNKAWTDAYRGAGRPEATFAIERMMDE
                     LAVEVGVDPLEIREKNWITHEEFPFRTVAGLDYDTGNYEAATERAKSLFGYDDLRAEQ
                     KRRRESGDPVQLGIGISTFTEMCGLAPSRWLGTNGYVGGGWEHASVRMLPTGKVEVIA
                     GTSPHGQSHETAWSQIVADRLGVAFDDVEVLHGDTQVSVRGLDTYGSRSLAVGAMAVV
                     AAADKVIERARPIAAHLLEANPDDLEFSGGSFTVKGTDASKSLAEHAFAVFQGHNLPD
                     GVDRSLDAEATFDPDTFSFPHGTHLCAVDVDTETGEVKIRNYTSVDDVGKVINPLIVE
                     GQAHGGIVQGVSQALFEEAVYDDTGTLVTGSFVDYLVPSAADLPTITAERTESPSTTN
                     ALGVKGVGETGTIASTPAVVNAVIDAVRHLGVDDVAMPMSPHRVWTAIQHGSKGAATP
                     TDNPTVDDPALPSHNAATAEGGQS"
     gene            complement(7288..7755)
                     /locus_tag="VH419_00355"
     CDS             complement(7288..7755)
                     /locus_tag="VH419_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020387577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEY7042097.1"
                     /translation="MVRINVTVDGVAYSDDVEPRTLLVQYLREQLGKIGTVVGCDTSN
                     CGACTVHLDGKSVKSCTMLAVQADDHDVTTIEGLASNGTLHPMQQAFHENHALQCGYC
                     TPGMIMQSIALLEDNPHPDEAAVREGLEGNLCRCTGYQNIVRAVLAASKAGAQ"
BASE COUNT         1052 a         2755 c         2750 g         1413 t
ORIGIN      
        1 gacgatctcg cccgaggact ggggcgtcgt acgggggtcg accgcctcgt tctcggtgcg
       61 gcgatcgttc tgctggccgg cggtgccacc gcgctggccg gcccgatcgc gttcgtcggc
      121 ctcgtcgtcc cacacggcgt ccgagctgtc gtcggtacgg actaccgcag cctgctgccc
      181 ctcagcgccg ggtacgccgc agcgctgacg gtcgtcgccg acaccgtcgg ccgggtcgtg
      241 ctgccgccca ccgaagtgca agtcggcatc atgacggctg tcgtcggtgt cccggtgttc
      301 ctctggctcg tccgacgcgg caggacgggg ctctgatgac cctgctggac gtcgcaccga
      361 cgcgcaggcc ggatcgcgcc ctcgagctgt cccgccgagc gcgccgtgga cccgtgcgtc
      421 ggcagcggct ggtgatctgc ggtctcgtgc ttgccctgct gctcgcgttc gcggcccgtg
      481 tcctgctggg cgacttcacg gtgacgatcc cggacttcgt ccgcatcctc ttcggcgccg
      541 acattcccgg cgcgtcgtac atcgtgatgg agaccaagct gccacgtgct gtgctgggtg
      601 tcctcgtcgg cgcctccttc gggatcggcg gcgcgatctt ccagacgacg ttgcgaaacc
      661 cgctcgccag tccagacatc atcggtgtga gcgtcggcgc cagcgcggcg tcggtcttca
      721 cgatcgtcgt gctcggcagc tcggagtcca gtgtctcgtt cgcggcgatc atcggagcgg
      781 tcctggtcgc cgtcacggtc cgtgtggtcg ccggccccgt ccgggggtac cgactggtcc
      841 tcgtcggtgt cggggtggct gcggcgctcc agtcggtgat ccagtacttc ttcacccgcg
      901 ccgacgtgta cgacgcccag gctgccctgc gctggctgac cggcagcctc agcgcagcgg
      961 attggcccgg catcacgatc ctggccgtgt tcttgttgct gtgccttccg atcatcgtgt
     1021 ggctggcccg gtccctgccg gtgatcgagc tcggacccga cgcggcagcg agtctgggtg
     1081 tgacgacgcg tcgcaccgac gcgctgttga tgctcgccgt tgtcctcact gctgtcggtg
     1141 tggcagcggc cggaccggtt gcgttcgtat cgttcctggc cggacccatc gcgcgcggtc
     1201 tcaacggcgg gcgtacgacg ctcgtgggtg ccgcgctgac aggcgccgtg atcgtcgtgg
     1261 gcgccgacta tgtcgcggac tacctgctgt ccgacgtcaa cttccccgtc ggcgtgatca
     1321 cgggagcctt cggcgcgccg tacctgctgt ggctgctcgc gcggcgtgaa aggagccagc
     1381 gatgacgctg gtcgaggacg acgtcgtgca tgtccggact cagcagcaac cggccgcgcg
     1441 gctgcgggcc gagaacgtca cccttggcta cgacgagcat ccggtcgtcg agtcgctctc
     1501 gctggagttc ccggaccgtc gggtcaccgc ggtgatcggt gcgaacgcgt gtggcaagtc
     1561 aacgttgctg cggggactcg gccgactgct caggccgcag gcgggcagcg tcctgctcga
     1621 cggggcggcg atccaccggc tccccacccg tgaggttgcc cgcacgctgg gactgctgcc
     1681 gcaggatccg gtcacgcccg acggtgtcac ggtcgtcgag ctcgtcgagc gtggccgcca
     1741 tccgcatcgc aacgcgttcc gccgtcgttc gggtgcagac accgaggccg tggccgcggc
     1801 gctcgagctg accgacactg ccgggctcgc cggccgcgtc atggacgagc tctccggcgg
     1861 acagcggcag cgggtgtgga tcgcgatggc gttggcgcag gaaaccgacc tgctgctgct
     1921 cgacgagccg accacctacc tggacctggc gcaccagctc gagatgctcg acctgctgcg
     1981 cgagctcaac gcagtccgcg gcaccacgat cgtcatggtg ctgcacgacc tgaacctcgc
     2041 agcccggtac gccgaccacc tggtggcgat gcgcaccggc cgcgtcgtcg ccgcgggtac
     2101 acccgccgag gtcgtcaccg agcagaacgt caaccaggtg ttcgacctcg actgccgcgt
     2161 catcagcgac ccggtggccg gcacgcccct ggtcgtgccc gtcgcccgcc gcggccagga
     2221 gttgtcggaa tgaccgcaac ggcgacggac gttcaccgcc ccgccccgat gatcgtcgac
     2281 caggtccggg tcgtgtctgt cgaacgcttg tccccctcgt tcgcgcgcat cgagctcggt
     2341 ggtccggcgc tgacggagtt cggcgtcgac ggaccgctct acgaccagcg cgtcaagctg
     2401 atctttcccg gcccgtccgg actgctgccg aggatcgatc cctatgacga cgggtggtac
     2461 gccgggtggc gccgcttgcc gacggaggtc cgcggccaca tgcgcacgta caccgtgcgt
     2521 acgacccgcg ggcgcggcgc gggcacgacg cttgtcgtcg atttcgtcct gcacgacgag
     2581 gcgggtgtga ccggtccggg cgcggcatgg gccgccggcg cgcaggtcgg ggactcgctc
     2641 gtcgtcgtcg ctccccggcg cggctggccg ttcggcggta tcgagttcgc accgttcccg
     2701 tcgtcgcgtc ggttgatgct ggtcgccgac gagaccgcgg tccccgccgt cggtggcatc
     2761 ctcgacggac tgccgtcagg ccttgtcggt acggcgtacc tcgaggtccc ggtgtccgcg
     2821 gacatcctcg acatgcacgc accgccaggc atcgacgtcg tttggcttcc tcgcgacggg
     2881 gccgagctcg gcacccggct ccgccgcgcg gtctcggcac agttcggggt cgccacagtt
     2941 gcagccgggc cacatgaggt cgacccggat ctgtgggaga cgccgacgta ctcctcgtcg
     3001 ggagagccca tcgacgttcg tccaccgacg accggtgcgg ggggtgacct gtacgcctgg
     3061 gtggccggcg agtctgcggt ggtgacgggt ctgcgccgtc agctggtgcg ggagctcggc
     3121 ctggaccgcc ggcaggtcgc gttcatgggc tactggcgcc gcggcgtcgc catgcaggca
     3181 tgagccgctc cgcagcagga gtcaggagcg cctgaggagc ctcttcagca gccagagggc
     3241 gatcagaccg ccgatgacgt agggcgcata ccgcttgacg aggacaggca tcacggtggc
     3301 gccgaggtcg agctcttctg tcggctcggc ggatgacgcc gacactgccg ccgcagcttc
     3361 cgcgccgatc ggcgtcgccg ccgactccgc agctgcgctc tccggctccg gaaccgtgct
     3421 ctggggctcc gcggtcgctg ccgaggcggt ggactcctcg ccgccgagct tggtctccag
     3481 acaggccgcg aactggccga gcagtttgtc gctgacgtct tgcatcacac cccggccgaa
     3541 ctgcgccggc cgcccggtga tgctgaggtc ggtcgtcacc gtgacgtcgg tggcgtcgtc
     3601 accactcgcc gccaatgtcg tggtgacgtt ggcggcggcg gtgccgttgc cccgcttgtc
     3661 cctccccttc gcctcgatga ccgcccggtg cgtcgcctcg tcgcgctcga cgaaccggcc
     3721 cgacccggcg tactggagcg agatcgggcc gagcttcacc ttgcagatgc cttcgaaggc
     3781 gtcgccgtcg tacgacgtca gcgacgcccc gggaaaacat ggcgcgatcc gttcgaggtc
     3841 gttgaacgcc ttccaggcga cgtcaacggg tgcagggacc gtgaactggt gcgtcagctc
     3901 catgagcgcg cctttcagtc gatgacgccc cgggcgatga cgtcgtcgtg tgcgtgggtt
     3961 ccgtcaggcg ttcacgaccc cgcggccgcc agcacggcgc gaccggtgag gacgctcgcc
     4021 aggtgtcgac ggtagtccgc atcgccgttc gtgtccgacg gcgggttggt cccttcggcc
     4081 gcctgagcgc acgccgcctt gacggcgtca gccgtggccg actggcccac gagtgcctgc
     4141 tccaccgcgg tcgcccgcag tgcggttgcg cccatgttgg tgagcccgat cttggccgct
     4201 gagatctgac cgttctccgc cttgaccgcg gccgcgaccg cgacgatcga ccactgctgc
     4261 gccacccggg tgaacttctc gtagtggccg ccccagccgg tgtgcttcgg gatgcggatc
     4321 ttcgtgagga tctcgtcctc gccgacgacc gtcgtgaaca ggtcctcgaa gaagtccgcg
     4381 gccgcgacgg tccgttcccc gccgggtccg gcgatcacga gctcggcgtc gagggcgagg
     4441 atcggcgccg gcaggtcgcc cgccgggtcg gagtgtacga gcgccccgcc gagcgtgccc
     4501 atgtgccgga cctgcgggtc ggccaccgac tgggcggcct cggcgaggac atgggcgtgg
     4561 tcgcgcacca gctcgttcgt ggccacctcg tcgtgcgtgg tcatggcacc gatgacgatc
     4621 gcgtcaccgt cctcggagat gcctttgagc tcgtcgatgt gaccgatgtc gacgagcaga
     4681 gtcggcgccg caagccgcag cttgaggacc gggatcaggc tctgcccgcc ggccagcacc
     4741 ttgatctcct cggcgtcgcc gccgagtgcg gtgatggcgt cggcgacggt cgtcggcgct
     4801 gtgtagtcga acgcggccgg gatcatgact gtcctccctc agcggtggcg gcgttgtgtg
     4861 acgggagtgc tgggtcgtcg accgtggggt tgtcggtcgg ggtcgcagca cccttcgacc
     4921 cgtgctggat agccgtccac acccgatgcg gcgacatggg catcgcgacg tcgtcgacgc
     4981 cgagatgacg gacggcgtcg atgaccgcgt tgacgaccgc tggtgtcgaa gcgatcgttc
     5041 ccgtctcgcc gacgcccttc accccgagag cgttggtcgt cgacggggac tccgtgcgtt
     5101 cggccgtgat cgtcgggagg tcggcggcgc tgggcaccag gtagtcgacg aaggagccgg
     5161 tgaccagcgt tcccgtgtcg tcgtagacgg cttcctcgaa cagcgcctgt gagacgccct
     5221 gcacgatccc gccgtgcgcc tggccttcga cgatcagcgg gttgatgacc ttgccgacat
     5281 cgtcgacgga ggtgtagttg cggatcttca cctcgcccgt ctcggtgtcc acgtccacgg
     5341 cgcacagatg tgtgccatgt gggaacgaga acgtgtcggg gtcgaacgtt gcctccgcgt
     5401 ccagcgaccg gtccacacca tcgggaaggt tgtgtccctg gaagacggcg aacgcgtgct
     5461 cggcgagcga cttggacgca tctgttccct tgacggtgaa agagcccccg gagaactcga
     5521 ggtcgtccgg gttggcctcc agcaggtggg cggcaatcgg acgcgctcgc tcgatcacct
     5581 tgtcggctgc ggcgacgacc gccatcgcac ccacggccag cgaccgagag ccgtaggtgt
     5641 ccagcccccg gacggacacc tgggtgtcac catgcagcac ctcgacgtcg tcgaacgcca
     5701 cgccgagccg gtcggcgacg atctgcgacc acgccgtttc gtggctctgc ccgtgcggac
     5761 tcgtcccggc gatgacctcc accttgccgg tcggcaacat cctgaccgac gcatgctccc
     5821 agccacctcc gacgtacccg ttcgtgccca gccaacgcga cggcgccaga ccgcacatct
     5881 cggtgaacgt cgagatgccg atcccgagct ggaccgggtc gccgctctcg cgacgccgct
     5941 tctgctcggc ccgcaggtcg tcgtacccga acaggctctt ggcccgctcc gtggcggcct
     6001 cgtagttgcc cgtgtcgtag tcgagtccgg cgaccgtcct gaacgggaac tcctcgtgcg
     6061 tgatccagtt cttctcgcgg atctcgagcg ggtcgacccc cacctcgaca gcgagctcgt
     6121 ccatcatccg ctcgatcgcg aacgtggcct ccgggcgacc ggcgccgcgg tacgcgtcgg
     6181 tccacgcctt gttggtgaac acgttggtcc cggtccagcg gtatgccggg aacttgtaga
     6241 tgccgttgaa catcaacgcg ccgaggaacg ggatggccga cgtgaccagt ccgaggtagg
     6301 cgcccatgtc gcacagcagg tccaccttga agcccgtcac cgtgccgtcc ttgtcggccg
     6361 ccagggtcag ggtctgccac tggtcgcgac cgtggtgggc gaccatcagt gactcgctgc
     6421 gcgtctcggt gtacttgcac ggcttcccgg tatgacgtga ggcgatgacg gtcaggacct
     6481 cttccggcgt gaactgcagc ttgcctccga agccgccgcc cacgtcggga gcgatcaccc
     6541 ggatcttgct ttccgggata ccgagcacca gcgagatgaa gatcttgacg aagtgcggta
     6601 cctgcgtcga cgtccagacc gtcatctgct cacccgtggg atcgacgacg accgaccggg
     6661 gctccatgaa cgcggggatg agccgttgct ggcggaactc tcgttcgatc acgatgccgt
     6721 tctcccgggc cttcgcgatc gcgtcgtcga cgtcgcctcc cgtgcctgcc tcggcggagt
     6781 cgaaggtccg taccgccgag atgttggtcc caagatcggg gtgggccaag acctcgttgg
     6841 ccgcggccgc cttcaggtcc aacgcgaccg gcagcgcctc gtagtcgacc tcgaccagct
     6901 cagcggcgtc acgcgcttct gcagcgctgc gggcgacgac gatgccgacg acctcgccgg
     6961 cgaaggccac ccggtcgacc gcgatggacg ggtggttggg agacacctgt tcgggggtga
     7021 gcatccaggc gttcggcagt ccgccctgtt cgtcggcaag gtcggcaccg gtgtagaccg
     7081 ccacgacgcc gggcgcgcgc ttggcctcgt cggcgttcac cgaagtgatc cgtgcgtgcg
     7141 cgaacgggct tcgcaccatc gccaggtgca gcatcccggg cagcttgatg ttgtccgtcc
     7201 agcgggtacg cccggtgatc agccgcgcat cctccttgcg gcggcgggcg gtgccgacct
     7261 cctgcgtcgg ccgttcttcg gtgacggtca ctgcgcacct gctttcgacg cggccagtac
     7321 ggcccggacg atgttctggt atcccgtgca ccggcacagg ttgccctcca ggccttcacg
     7381 gaccgccgcc tcgtcagggt gcgggttgtc ctcgagcagg gcgatgctct gcatgatcat
     7441 gccgggtgtg cagtagccgc actggagagc gtggttctcg tggaacgcct gctgcatcgg
     7501 gtgcaacgtg ccgttgctgg cgagaccctc gatcgtcgtc acgtcgtggt cgtcggcctg
     7561 gacggcgagc atcgtgcatg acttcacgct cttgccgtcg aggtggacag tgcaggcgcc
     7621 gcagttgctc gtgtcgcaac cgacgacggt gccgatcttg cccagctgtt cccgcaggta
     7681 ctggacgagg agggtacggg gttcgacgtc gtcggagtac gcgaccccgt cgacggtgac
     7741 gttgattcgg accatggtcg tcgcctctca ccgaaggtcg gtagggatgg ttcggacgct
     7801 aggtcagggc gcgttgcacc gccgttgcac gcagccgctg cttcgtgact ggtcggtcac
     7861 ccggcgtgat gaatggggcc ttcggacgcc ttcagcgggc ttccgtcgcc accccagcgc
     7921 agcgcgatga tctcggccgc gacgctgatc gcggtctcct ccggcgtacg
//
LOCUS       DATBFZ010000013         1872 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_4269786, whole genome shotgun sequence.
ACCESSION   DATBFZ010000013 DATBFZ010000000
VERSION     DATBFZ010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 1872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1872
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4269786"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..974)
                     /locus_tag="VH419_00360"
     CDS             complement(<1..974)
                     /locus_tag="VH419_00360"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY7042098.1"
                     /translation="METPTRPGQLVGIGVVGYGYWGPNLVRNFGTTPGARMVAVSDLS
                     PTRLASVQALYPAVKTTPRYEDMLLDPEIDAVAIATPVSSHFRLAMQALEAGKHVLVE
                     KPLALTSGQAALLVEEADRRDLVLMVDHTFVYHGSVQRIRELIESGQVGKLHYFDSTR
                     VNLGLFQRDVDVLWDLAVHDLSIMDYLLGKSPVAVAATGAAHVPGQPVNIAYLTCFFE
                     GSLIAHVNVNWLAPVKVRRTLIGGDRQMIVYDDLEPSEKVKVYDSGVTLGDGPAGPDG
                     PYERLVGYRTGDMWAPKVSSTEALAVEAEHFVDCIVHGTQPLSSGQVGL"
     gene            1384..1779
                     /locus_tag="VH419_00365"
     CDS             1384..1779
                     /locus_tag="VH419_00365"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY7042099.1"
                     /translation="MPYRINHIHLKAPDPKKTADWYVKAFNFKIVSDEVRVFGDRFVR
                     CQSEDGGMAVNISGARTGETLGSGDANPHHGLEHFGLDTDDIEAEIARLEGLGATRME
                     GPIQMANGGKIAFIKAPDDVRLELIQAPK"
BASE COUNT          318 a          650 c          607 g          297 t
ORIGIN      
        1 agaccgacct gcccgctcga gagcggctgc gtgccatgca cgatgcagtc gacgaagtgc
       61 tcggcttcga cggccagcgc ctcggtggag ctgactttcg gcgcccacat gtcgccggtc
      121 cggtagccga ccagccgctc gtacgggcca tccggcccgg ccggcccgtc gcccagcgtc
      181 acgccgctgt cgtagacctt caccttctcg ctcggctcca gatcgtcgta gacgatcatc
      241 tggcggtcac cgccaatcag cgtccggcgg accttgaccg gcgccagcca gttcacgttg
      301 acgtgcgcga tcaggctccc ctcgaagaag caggtcagat aggcgatgtt gactggctgg
      361 cccggcacgt gcgcggcgcc ggtcgcggcg accgcgaccg ggctcttgcc aagcaggtaa
      421 tccatgatcg acaggtcgtg gacggccaga tcccagagca cgtcgacgtc acgctggaac
      481 aggcccaggt tgacccgagt cgagtcgaag tagtgcagct tgccgacctg gccggactcg
      541 atcagctcac ggatgcgctg gaccgagccg tggtagacga acgtgtggtc caccatcagc
      601 accaggtcgc gccgatcggc ctcctccacc agcagcgccg cctggccgct ggtcagcgcc
      661 agcggcttct cgaccagcac gtgcttgccg gcctccagcg cctgcatcgc cagacggaag
      721 tggctggaga ccggcgtcgc gatggcgacg gcgtcgatct ccgggtccag cagcatgtcc
      781 tcgtagcggg gcgtcgtctt gacggccggg tagagtgcct gaacgctcgc gagccgggtg
      841 ggcgagaggt cgctgaccgc caccatgcgc gcgccggggg tcgtgccgaa gttgcggacc
      901 aggttcgggc cccagtagcc gtagccgacc acgccgatgc cgaccagttg ccccggacgg
      961 gtcggcgtct ccacggatcg ctcgaacggt acagtcgcta gcggcggagt cgagtcggtt
     1021 ggcggcacca ccctgtcatc ccctcacgtc gttcgaccag ctcacgcggc cgtcgcgtct
     1081 ccagccacgc gtcacgcgtc aaccagcctg ccgaggtgct gtcatggtcg agaaacgagc
     1141 gtcaagaccc gctgtctcat taccatccgt aacggatcgt ggcgcctcaa ggtgccggct
     1201 cgttcatgat acgcggcgca ggtccgcatc gtggcccgcc ccgagatact gtgaggcgct
     1261 cgtggcctcg tggtcctgtc cttgacgact cgtcccccat cgcctaccgt tccgcccagg
     1321 cgtgggctcg cacccacgcg gcgaacatgc tgcgagccca tgacgtcacc tgggagagcg
     1381 acgatgccgt accgtatcaa ccatatccac ctgaaggcgc cagaccccaa gaagacggcc
     1441 gactggtacg tcaaggcgtt caacttcaag atcgtcagcg acgaggtccg cgtcttcggc
     1501 gaccgtttcg tgcgctgcca aagcgaggac ggcgggatgg ctgtcaacat ctccggcgct
     1561 cgcacgggcg agacgctcgg gtcaggcgac gccaacccgc atcacggcct ggagcacttc
     1621 gggctcgaca ccgacgacat cgaggccgag atcgcccgcc tcgaagggct cggcgcgacc
     1681 cggatggagg gcccgatcca gatggccaac ggcggcaaga tcgccttcat caaggctcca
     1741 gacgacgtcc ggctggagct gatccaggcc ccgaagtaga cgaaggggca gtcggcagtc
     1801 ggtagacatc cacgcccatc atccgtccgt ggactgatga agtccacgtc tacgttcggt
     1861 ggtcgctgcg cg
//
LOCUS       DATBFZ010000014        13789 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_4926617, whole genome shotgun sequence.
ACCESSION   DATBFZ010000014 DATBFZ010000000
VERSION     DATBFZ010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 13789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13789
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4926617"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="VH419_00370"
     CDS             complement(<1..533)
                     /locus_tag="VH419_00370"
                     /inference="COORDINATES: protein motif:HMM:NF039494.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042100.1"
                     /translation="MPHLTIAAIYQAARTAGFTPEQATTWTAIALAESGGNTGALNDH
                     GEYSMGLWQINVGPGVRDNVWGNLNDPVTNARAAYAISHHGTDMRPWTTTHDSNQGTS
                     ADYRTYLSQVEAVTGVQGDDRGVGGYDSPLPPPLPSSGDPGLVQPSTLTDTLTYDQID
                     GGMLPGANVDTDLDGLT"
     gene            688..3282
                     /locus_tag="VH419_00375"
     CDS             688..3282
                     /locus_tag="VH419_00375"
                     /inference="COORDINATES: protein motif:HMM:NF012242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEY7042101.1"
                     /translation="MGYRLGIDLGTTFTAAAIGNGQPPTMLGLGNRALQIPSVLFLRD
                     DGSFLVGEPAERRGLVEPGRLVREFKRRIGDHVPLLVAGTPYSPQALTARLLSWVVAT
                     STERVGTAPDSLVVTYPANWGAYKRELLDQVITLADVGQAYTCPEPQAAAAQYAARAG
                     LESGGRIVVYDLGGGTFDVCVLMKTDSGFTILGSPEGIEHLGGIDFDEAVFRHVLTSL
                     KSQLAGLDPESPDVRATLSRLRRDCVDAKEALSSDVDTAIPVALPGGGTTVRLTRSEL
                     ESLIGPSLQDTISATARALRTADTTPEQLTSIVLVGGSSRIPLVSHLLQQHFGTTTAL
                     DTHPKHDVALGAVQYQPDGGPAERPPPVGGPTTQAAGAGSERPTTPSRLSPQEQARRS
                     GLPVPPPARPAASELAGPSAQPAVEELPVAPPVEDAVEDAREDAAPPPPPVAGSRPGP
                     QPTAPPPVSGPPPDATLTGPPQSPRPPRRSPVEWFSDASTWQKIGTVVAVVGVIAAGA
                     VVVRLASGSSPAASTSGGPETSPSTPTNGQSTAPHAPGLPGSDPLPETSVVVPMETKR
                     GDSNLYAVDTSRPGSRPKPLAVGPADETLPALSPDRKTVIYIKTPYSTEEGGIPENAV
                     STMWAMPAVPAAGDEPVRLRYRLNGSPCVSRPAWSPDASLLAVLCGPEEKTAIGLVHL
                     AEDGPAVVTPLMRIDAREHPDGIEDLTFSGDGTQVIFADHANSGNTTGLYAVDVKSGA
                     TTRLTDGLDKDGDSSPVNPDMLAFRRADSLGAADIFLLSLSGGRIPCPYETSQEGPYT
                     LCRLTDVGRHVAQDPSWSQDGSQIAFKWNTEDADLKVVPVSPTIVQPESVLPETHGGA
                     KAPAWSAR"
     gene            complement(3331..3882)
                     /locus_tag="VH419_00380"
     CDS             complement(3331..3882)
                     /locus_tag="VH419_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012919431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEY7042102.1"
                     /translation="MTFHTGQLLLATPRLLDPNFARSVIFLLDHDEDGALGVVINRPS
                     QLPLSAVLPTWSTVVTAPPLLFSGGPVSPESALAVGLSLGDGPGVGFKRLTGDYGLVD
                     LDADPSEVLADLVGVRVFSGYAGWGSGQLEAELVEGSWYIAPALPRDLLDPDPEHLWR
                     TVLRRQPGELAYVATFPDDPTMN"
     gene            complement(3893..5287)
                     /locus_tag="VH419_00385"
     CDS             complement(3893..5287)
                     /locus_tag="VH419_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020387088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HEY7042103.1"
                     /translation="MPAAPSVSYSITVRLVVPAGGTAVSQLTNAVEGAGGIVTALDVT
                     ASGHDQLRIDVTCAARDTDHADRIVEALRGVEGVVVHKVSDRTFLMHLGGTIEMASKH
                     PIRNRDDLSMVYTPGVARVSMAIARNPEDARRLTVKRNAVAVVTDGSAVLGLGNIGPA
                     AALPVMEGKAALFKRFAGIDAWPLCLDTQDVDEIVRTVQLLAPVFAGINLEDISAPRC
                     FEVEARLRKLLDIPVFHDDQHGTAIVVLAALTNALRVVGKSLDGVRIVMCGAGAAGSA
                     ILRLLLESGAKDLVVADVDGVISTKRSGMNSSLSWIAENTNAACFEGTLKEAVRGADV
                     FIGVSAPDLLDGGDIESMAADSIVFALANPDPEVDPAQARRYAAVVATGRSDYPNQIN
                     NVLAFPGVFRGLLDASANAIEDSLLIAAAEAIAGTVSDEELNANYVIPSVFHEEVHKA
                     VATAVKDAAHAVGA"
     gene            complement(5731..6744)
                     /locus_tag="VH419_00390"
     CDS             complement(5731..6744)
                     /locus_tag="VH419_00390"
                     /inference="COORDINATES: protein motif:HMM:NF022702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HEY7042104.1"
                     /translation="MPGRRHTLVAAGTLVSLSLVLTACGGDEGSAEDPTTPVSTANAL
                     LLNGEWPLTGEKLDGDLPKHPVYVVKIDNTTNSAPQHGLDSADMVAEELVEGGFTRLV
                     AFYYQDAPGLVGPVRSMRASDIPIVKPTNAVIIAAGGAASTKRRVAAAGISNLGEGSP
                     GFERDDSRSAPYNLYAHLSQIAAHPTGRWEPPAGPYFQFGDTDLTDARQVTKISAEFS
                     GAHTTDWVHTDKGWVRTNSNAEKGHDFSVDTVLLLRVREGDAGYLDPAHNPVPETLLH
                     GSGAAILVHGDKALVCRWQKTKDPASTLVLTTKSGHAVNVPPGHTFVELVPAETGKVT
                     LTK"
     gene            complement(6975..7481)
                     /locus_tag="VH419_00395"
     CDS             complement(6975..7481)
                     /locus_tag="VH419_00395"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEY7042105.1"
                     /translation="MPATSVRRRSYVAVPVMLLTTLLVTLSMVVTTANPADASMRQQR
                     RAHQIQHGLRVAIDQIGDPYVYGADGPGAFDCSGLVMFSYKRAGVYLPRSSSAQAHYV
                     RHIPKSHLHRGDLMFFYDGGGVYHVGIFLRRDHGRPILLHAPYPGRRVHREHVWTHRW
                     FAGTLRLR"
     regulatory      complement(7485..7665)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            complement(7934..8575)
                     /locus_tag="VH419_00400"
     CDS             complement(7934..8575)
                     /locus_tag="VH419_00400"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN reductase"
                     /protein_id="HEY7042106.1"
                     /translation="MTRRIVVVSGGLSEPSTTRLLADRLAASVEKSLTDERLIVGRQV
                     DGLEVSVVELRDVARDITDALLTRVPSAALQTVIDAVTSADGLVAVSPVFNASYSGLF
                     KSFFDVLDADDLAGTPVLIAATGGTERHSLALEHALRPLFSYLHALVVPTAVFAATSD
                     WGSREYSARLSARIDRATGELARLVAEAPAVAQPTDPYDDPTPFEELLFGSPS"
     gene            complement(8572..9714)
                     /locus_tag="VH419_00405"
     CDS             complement(8572..9714)
                     /locus_tag="VH419_00405"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012917980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY7042107.1"
                     /translation="MQFGIFSVGDVTTDPTTGRTPTEYERIKATIEIAKKAEEVGLDV
                     FATGEHHNPPFIASAPTTTLAFIAARTERIILSTATTLITTTDPVLIAEDYAKLQHLA
                     DGRVDLMMGRGNTGPVYPWFGKDIRDGIPLAIENYHLLHRLWREDVVDWEGRFRTPLQ
                     GFTSTPRPLDGVPPFVWHGSIRSPEIAEQAAYYGDGFFANHIFWPKEHFMRLIGLYRA
                     RFEHYGHGSADQAIVGLGGQVFMRKNSQDAIREFRPYFDNAPVYGHGPALEEFMEQTP
                     LTVGSPEQVIEKTLTFREHFGDYQRQLFLMDHAGLPLKTVLEQLDMLGADVVPVLRRE
                     LDGLRPAHVPDAPTHQSLLAAAKTVSAEAVSDDTAADQAELEESKR"
     gene            9933..10136
                     /locus_tag="VH419_00410"
     CDS             9933..10136
                     /locus_tag="VH419_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008357790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1918 domain-containing protein"
                     /protein_id="HEY7042108.1"
                     /translation="MFAAVGDRLTVHSQHLDGVVRDGEIIEVHGDQGQPPYLVRWSDD
                     GHEALVFPGPDASVEHGEHSATE"
     gene            complement(10173..11333)
                     /locus_tag="VH419_00415"
     CDS             complement(10173..11333)
                     /locus_tag="VH419_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017935467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HoxN/HupN/NixA family nickel/cobalt transporter"
                     /protein_id="HEY7042109.1"
                     /translation="MTTTTGTTRTPTASAPPPGSRWHRFRTSLTPAEWRRAAGLFAVI
                     LALHVIGFGLLFALVVPGNFSLGDGSLFGVGVGITAYTLGMRHAFDADHIAAIDNTTR
                     KLMTDGQRPLSVGFFFSLGHSTNVFVLALLFSWGIRGLSGEVSDDSSSLHKATGVIGP
                     SVSGVFLLLIGILNLLVLVGIVRVFRRMRHGMYDERELEDQLNNRGFMNRFYGRATNA
                     IKKPWQMYPLGVLFGLGFDTATEIALLATAGAAAAGGLPWYAILCLPILFAAGMSLLD
                     TIDGAFMNFAYGWAFAQPVRKVFYNITVTALSVAVALIIGMVELVAVFSEKFGLEDGV
                     VGWVAALDLNYVGYAIVALFVVTWLTALAVWRFGRIEEKWSSGLRQAERAVD"
     gene            complement(11518..>13789)
                     /locus_tag="VH419_00420"
     CDS             complement(11518..>13789)
                     /locus_tag="VH419_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042110.1"
                     /translation="KLAGAEALVVLDNCEHLVDGVSVFVERLLSACPEVTVLATSRVR
                     LQVPFESVFQVPGLSLDDGTGEGDSDAVTLFVDRAAMAGWSSSYRYDQRRITAICEQL
                     DGVALAIELAAARLTTLGLDGLEIGLADRLDLLAGGPRLDARHRSVRSALDWSFGLLG
                     EKDQVVLRRTSVFAAPFTSERAVAMAGHAPLSPSDVAVSLARLTDQSLLVVSSELDGT
                     HYRMLETIRQYGTERMVDVGEETDVRDRHLRWCLATAASQAAADGGPPAAFEEVADDL
                     RAGLRWAGCRPELRADAHGLALLLARLAYTSGLPSEAQERYEEAAALAPEPADAAHAL
                     HLAAAVAWGRHLGNDAMRLYRAAAEAARRAGDPHRAALELLCAAELITNAPGLMSELA
                     PPGEEQALLAEARALAGGDLHVEAAVLTVTTPNDEVDPGYAELAERAADLAHRVGDVR
                     LESHALDQLTAARFISGEVGNAVATIRRRIDLLTPRAHDVELAWEYSDTLHMAPLVYV
                     AAGDLETARRHAQERSELSFFREADHLAVEWLLTTAAIAGDFDEASALAQRFRRAWEE
                     AGRPSIGGIAFAPAAAAMVYGIRGDDDARRDWLDITAEMSRVVEHMRGRRTIYSPTFE
                     GMVALHRGEIAAALTHVAGEPESFKPWHDAAWRPWYAAVWAEAGVLAALPDRRSRLDR
                     ARFVVRENPISSALVDRAAAIDADDTEMLLAAADALSTAGCRYQHARTLVFAGGDARA
                     TGEAIMADIGAAPMTA"
BASE COUNT         2251 a         4664 c         4699 g         2175 t
ORIGIN      
        1 ttggtgagac cgtccaggtc ggtgtcgacg ttggcaccgg gcagcatccc gccgtcgatc
       61 tggtcgtagg tgagcgtgtc ggtcagcgtg ctcggctgga ccagcccggg atcgcccgac
      121 gacggcagcg gaggtggcag cggcgagtca tagccgccga cgccgcggtc gtcgccctgg
      181 acacctgtca ccgcctccac ttgcgaaagg taggtccggt agtccgccga ggttccctgg
      241 ttggagtcgt gcgtcgttgt ccacggacgc atgtcggtgc cgtgatggga gatggcgtac
      301 gccgcccggg cgttcgtgac gggatcgttg aggtttcccc agacgttgtc ccggacgccg
      361 ggaccgacgt tgatctgcca caagcccatc gagtactcgc cgtggtcgtt gagggctccg
      421 gtgttcccgc cggactcagc gagcgcgatc gccgtccagg tcgtcgcctg ctccggagtg
      481 aaccccgcag tgcgagcggc ctggtagatc gcggcgatgg tcagatgcgg cattggctgc
      541 tcccgaggtg cgtccgcccg cacccgagcg tgaccacctc ttgtcagtgt aggtcggcgt
      601 catcgagagt aaccaccact tcaccaatcg tcgtcgtcga cgatgccctg gctctacgat
      661 gaggatgctt gagggggacg gactcacatg ggctatcggc tgggcatcga tctcggcacg
      721 accttcaccg ccgctgccat cggcaacggt cagccaccga ccatgctcgg actcggaaac
      781 cgcgcactcc agatcccctc cgtgctgttc ctgcgcgacg acggctcgtt cctggttggt
      841 gagcctgcag aacgtcgtgg cctcgttgag ccgggtcgac tcgtccggga gttcaaacgc
      901 cggatcggcg atcacgtgcc gttgctggtg gcgggtacgc cgtactctcc gcaggcgctg
      961 acggcccggc tgctgtcgtg ggtcgttgcc acgtcgaccg aacgggtcgg aaccgcaccc
     1021 gactcgctgg tcgttacata cccggccaac tggggcgcgt acaagcgcga gctcctcgac
     1081 caggtcatca cgctcgccga cgtcgggcag gcatacacat gtccggagcc gcaggcggcc
     1141 gccgcccagt acgccgctcg agcgggtctg gagtccggtg gccggatcgt cgtgtacgac
     1201 ctgggcggcg ggacgttcga cgtctgtgtg ctgatgaaga ccgacagcgg cttcacgatc
     1261 ctcggcagcc cggaaggcat cgagcatctc ggcggcatcg acttcgacga ggcagtcttc
     1321 cggcatgtgc tgacgtcact gaagtcgcag ctcgccggcc tcgacccgga gtctcccgat
     1381 gtgcgcgcca cgctgagccg gctgcgccgc gactgcgtag acgcgaagga ggcactgtcg
     1441 tccgacgtcg acacggcgat cccggtggca ctgccgggcg gcggcacgac ggtgaggttg
     1501 acccgatccg agctcgagtc gctgatcggc ccctcgttgc aggacacgat ctcggcgacg
     1561 gcgcgcgcgc ttcggacagc cgacacgacc ccggaacagc tcacctcgat cgtgctggtc
     1621 ggaggcagct cgcggatccc gctcgtcagt catctcttgc agcagcactt cggcacgacg
     1681 accgccctgg acacgcaccc caagcacgat gtcgctctcg gcgccgtcca gtaccagccc
     1741 gacggcggac cggctgaacg gccgccaccc gtcggggggc ctaccaccca ggccgccggc
     1801 gccggctccg aacgccccac gacgccgtcg cggctgtctc cccaggagca agctcgccgc
     1861 tcgggactgc cggtgccgcc cccggcccga ccggccgcat cggaactcgc aggtccgagc
     1921 gcccagccgg ccgtcgaaga actcccggta gcgcccccag tggaagacgc agtggaagac
     1981 gcaagggaag acgcggcacc gccaccgcca ccggtagcgg gttcccgccc tggtccgcag
     2041 ccgaccgcac cgccaccggt ctccggtcct ccgcctgacg cgacgctcac cggaccgccg
     2101 cagtcgccgc gtcccccgcg tcgttcgccg gtcgaatggt tctctgacgc gtccacctgg
     2161 cagaagatcg ggacagtcgt cgccgtcgtc ggcgtcatcg ccgcgggagc cgtcgtcgta
     2221 cggctcgcgt ccggctcgtc acccgccgct tccacgtcag gtggcccaga gacgtcgccg
     2281 tcaacgccca ccaacggcca aagtacggcg ccgcatgctc ctggacttcc gggctccgac
     2341 ccgttacctg aaacgtcggt cgtggtgccg atggagacca aacgcggcga cagcaacctc
     2401 tacgccgtcg acaccagccg accaggttcg cgtccgaagc ccctcgcggt cggccccgcg
     2461 gacgagacgc tgcctgcgct gtcgcctgat cgcaagaccg tcatctacat caagactccc
     2521 tactcaacgg aggagggcgg catacccgaa aacgccgttt cgacgatgtg ggcaatgcct
     2581 gcagtgccgg cggccggcga cgaaccggtc cggctgagat accggctcaa cggttcgccc
     2641 tgcgtcagcc gtccggcgtg gagccccgac gcttcgctgc tcgccgttct gtgtggcccc
     2701 gaggagaaaa ccgccatcgg tctggtccat ctggccgagg acgggccggc ggtggtgacg
     2761 ccactgatgc ggatcgacgc acgcgagcac ccggacggga tagaggatct gacgttctcc
     2821 ggtgacggta cacaggtgat cttcgccgat cacgccaact cggggaacac gaccggcctg
     2881 tacgccgtcg acgtgaagtc gggagcaacc acgcggctga cggacggcct cgacaaggac
     2941 ggggactcgt cgccggtgaa ccccgacatg ctggccttcc ggcgcgccga ctccctcggc
     3001 gcggcagaca tcttcctgct gagtctcagt ggcgggcgca tcccctgtcc gtacgagacg
     3061 tcgcaggagg gcccgtacac gctgtgtcgg ctcaccgacg tcggtcgtca cgtcgcccag
     3121 gatccgtcct ggtcacagga cggctcgcag atcgcgttca aatggaacac cgaggatgcc
     3181 gacctgaagg tggtgccggt gtcaccaacg atcgtgcaac cggagtcggt gctcccggaa
     3241 acgcacggcg gggccaaggc gccggcctgg agcgcccgct gaggctcgcg gcggtgacgg
     3301 ttcggtccgt gacggtcagt tcagtgacgg tcagttcatg gtcgggtcgt cggggaaggt
     3361 ggcgacatag gcaagctcgc ccggttggcg tctgagcacg gtccgccaca agtgctcggg
     3421 atcggggtcc agcaggtccc gaggcagggc cggtgcgata taccacgatc cttcgacgag
     3481 ctccgcctcg agctgccccg agccccatcc ggcgtacccg ctgaagactc gtacgccgac
     3541 caggtcggcg agtacctccg aggggtcggc gtccaggtcg acgaggccgt agtcgcccgt
     3601 gagccgcttg aagcccaccc ctgggccatc gcccaacgag aggccgacag cgagggcgga
     3661 ctccggcgac accggtccgc cgctgaacag caagggcggc gcggtcacga ccgtggacca
     3721 cgtggggagg acggcgctga gcggcagctg agacggacgg ttgatgacga cgccgagggc
     3781 tccgtcctcg tcgtggtcga gcaggaagat gaccgagcgg gcgaagttcg ggtcgaggag
     3841 tcgcggcgtt gccagcagca gttggccggt gtggaacgtc atcacagcct catcaggcac
     3901 cgaccgcatg agcagcgtcc ttgacggcgg tggcgaccgc cttgtgcacc tcttcgtgga
     3961 agacgctcgg gatgacgtag ttcgcgttga gctcctcgtc actgactgtg ccggcgatgg
     4021 cctctgctgc ggcgatgagc agcgagtcct cgatggcgtt ggcagacgcg tcgagcagcc
     4081 cccggaagac gccgggaaag gcgagcacgt tgttgatctg gttggggtag tccgaacgtc
     4141 ctgtggcgac gacggcggcg taccgtcgcg cctgcgccgg gtcgacctcc ggatccgggt
     4201 tggccagggc gaacacgatc gagtccgccg ccatcgattc aatgtcaccg ccgtcgagga
     4261 ggtccggcgc ggacaccccg atgaacacgt ctgcgccgcg gacggcctcc ttcaacgtgc
     4321 cctcgaagca cgccgcgttg gtgttttccg cgatccagct gagggaggaa ttcatgccgg
     4381 accgcttggt gctgatcacg ccgtcgacgt ccgcgaccac caggtctttg gcgccgcttt
     4441 cgagcagcag acgcaggatc gccgagcccg cggcaccggc tccgcacatc acgatgcgta
     4501 cgccgtccag ggacttgccg acgacgcgca acgcgttcgt cagcgccgcg agtacgacga
     4561 tcgccgtccc gtgctggtcg tcgtggaaga cagggatgtc gagcagtttt cggagccggg
     4621 cctcgacctc gaagcagcgc ggcgcagaga tgtcctcgag gttgatgccg gcgaacacgg
     4681 gagcgagcag ctggacggtc cggacgatct cgtccacgtc ttgggtgtcg aggcagagcg
     4741 gccacgcgtc gatgcccgcg aaccgtttga acaaggcggc cttgccctcc atcaccggca
     4801 gcgcggccgc cggaccgatg ttgccgaggc cgagcactgc tgacccgtcg gtcaccacgg
     4861 cgacggcgtt gcgcttcacc gtgaggcggc gcgcgtcctc cgggttccgc gcgatcgcca
     4921 tcgagacgcg ggccacaccg ggggtgtaga ccatggacag gtcgtcgcgg ttgcgtatcg
     4981 gatgcttcga cgccatctcg atcgttccgc cgaggtgcat gagaaacgtt cggtccgaga
     5041 ccttgtgcac gaccacgccc tcgacgcccc gcaacgcctc gacgatgcgg tcggcgtggt
     5101 cggtgtccct ggccgcgcac gtgacgtcga ttcgaagctg gtcgtggccg gaggcggtca
     5161 cgtccagggc agtgacgatc ccccctgccc cctcgacggc gttggtgagc tggctcaccg
     5221 ccgtgccacc agccgggacg acgagccgaa cggtgatcga gtaggacaca gacggcgcag
     5281 cgggcatgtg cccatcatcg caaacctcgg ccctcctcag ttttgaactg tcccgtgtgc
     5341 tgcctcgccg tggtgggttg cctggccagc catccgaatg cctggttcgc caggcaggtc
     5401 tgtggactgc tcgggtttgc ttggcgacgg agacgctcgc gatggatggc cccgatcggc
     5461 cggctgtccc ctgtcacggg ctggggtgga tgggcgctga agcgcgggca acgttcgcgc
     5521 ctgcgagctg gtcatccggc gttccgccct caggtcgatg tgcgagtagc gggtttcgga
     5581 cgtccgctcg tccgcggtcg cggtttgcat ggctaaccag ccaaacccat ccctcccgag
     5641 gcagatctgc ctggctaccc gtccgattgg atggctagcc aggcaaacca ccaacggtgt
     5701 cggtggatcg gagcggggct gcggcgatga ctacttcgtc agcgtgacct tgccggtctc
     5761 ggccgggacg agctcgacga aggtgtgtcc cggtggcacg ttgacagcgt gaccggactt
     5821 cgtggtcagc accaaggtgc tggcgggatc cttcgtcttc tgccaacggc acaccagcgc
     5881 cttgtcaccg tgcaccaaga tggcggcgcc agatccgtgg aggagcgtct cggggaccgg
     5941 gttgtgcgcg gggtccaggt agcccgcgtc gccctcacgc acccgaagca gcagcacggt
     6001 gtcgacgctg aagtcgtggc ccttctcggc gttgctgttc gtacgcaccc agcccttgtc
     6061 cgtgtgcacc cagtcggtcg tgtgcgcccc ggagaactcc gcactgatct tggtgacctg
     6121 ccgcgcgtcg gtgagatcgg tgtcgccgaa ctggaagtac ggtccggccg gcggctccca
     6181 ccgccccgtc ggatgtgccg cgatctgtga caggtgcgca tacaggttgt acggcgccga
     6241 ccggctgtcg tcacgctcga aaccgggtga gccctcgccg aggttgctga tccccgccgc
     6301 cgccacgcgt cgtttggtcg acgccgcacc accggccgcg atgatgaccg cgttcgtcgg
     6361 cttcacgatc ggaatgtcgc tggcccgcat ggagcggacc gggccgacga ggcccggggc
     6421 gtcctggtag tagaacgcca ccaaccgggt gaatccgcct tcgacgagct cctcagccac
     6481 catgtccgcc gagtcgaggc cgtgctgtgg cgccgagttc gtcgtgttgt cgatcttgac
     6541 gacgtacacc ggatgcttcg gcaggtcacc gtcgagcttc tcgccggtca gcggccactc
     6601 gccgttgagc aggagcgcgt tcgccgtgga gaccggtgtc gtcgggtcct cggcggagcc
     6661 ctcgtcccca ccgcacgcgg tcaggacgag cgagagcgac accagggttc cggcagcgac
     6721 gagcgtatgg cgacgtccag gcatgcgaag agatcctcga aagtgcagcg gttgacatgg
     6781 cattgggctc cagtgtgacg caggaccgcc cccggatcgg ggacggtcct ggcgcggggt
     6841 gtcacggatc acgcccctca gcccttcggc gccggcgtac ggcgggtcag tccttcggcg
     6901 ccggcgtacg gccggtcagc caacggaggc ggcccgagga aagcggttcc aggaagcggc
     6961 ccgaggagag ccactcagcg caagcgcagt gtccccgcga accaccggtg ggtccagaca
     7021 tgctcacggt gcacccgacg acccgggtag ggcgcgtgca gcaggatcgg tcggccgtgg
     7081 tcgcgcctga ggaagatgcc gacgtggtag acgccgccac cgtcgtagaa gaacatcagg
     7141 tccccgcggt gcaggtggct cttcggaatg tgccggacgt agtgcgcctg cgctgacgag
     7201 ctccgcggca ggtacacgcc cgcgcgcttg taggagaaca tcacgaggcc ggagcagtcg
     7261 aacgcacccg gtccgtcggc gccgtagacg tagggatcac cgatctggtc gatggccaca
     7321 cgaaggccgt gctggatctg gtgcgcccgg cgctgctggc gcatcgacgc atcagccggg
     7381 ttggcggttg tcaccaccat cgagagagtc accagcaacg tggtgagcag catgacaggg
     7441 acggcgacgt acgaacgccg ccgcacagaa gttgcgggca tgacagaggc tccttctcac
     7501 gcctgtgagg tcagctgtcg ggttcgggcg agaaggtgcc cggccacatc gaccgatcgg
     7561 agtactccga tctcgtggct acaccccgag cacgtgggcc gcgaacggcc gctgtcgtgc
     7621 agattgggtc ccccactcct gcccctggtg agatgagttc tcgactacag ggaatggcac
     7681 gaacgacgtg ggacaggact cggcgtacga cgctcgtgct gttcagttgt gacgcttgaa
     7741 gtgcccggtg accttaacga gtaacgcgtc cggatcaaaa cccgaaatcc ggcaatccgt
     7801 gtcaagacga agggaccgtg ggatagatca cgatcgacat cgacgcatcg tggtcgatct
     7861 gtcacgtgtc gtgttcaccc cacgccccgg gctgtacggc gtgcacctgg ttgccggcga
     7921 tggcgaggac cgatcaggaa ggggagccga aaagcagttc ctcgaacggg gtcgggtcgt
     7981 cgtagggatc cgtcggttgc gccaccgcag gtgcctccgc aaccagccgg gcgagctcgc
     8041 cggtggcgcg gtcgatccgt gccgacaacc tcgcggagta ctcacgggat ccccagtccg
     8101 acgttgccgc gaagacagcg gtcgggacca ccagcgcgtg gaggtaggag aagaggggcc
     8161 gcaacgcgtg ctccagagcc agcgaatgac gctcggtgcc ccccgtcgcg gcgatcagta
     8221 ccggggttcc ggcgaggtcg tcggcgtcga gcacgtcgaa gaacgacttg aacaacccgc
     8281 tatacgacgc gttgaagacc gggctcaccg cgacaagacc gtccgccgac gtcaccgcgt
     8341 cgatcaccgt ctgcagggca gcgctcggca cccgggtgag gagcgcgtcg gtgatgtctc
     8401 gcgccacgtc acgcagctcg acgacggaca cttcgaggcc atcgacctgt cgcccgacga
     8461 tcagtcgttc gtcggtcagc gatttctcga ccgaggcggc aaggcgatcc gccagcaacc
     8521 gtgtcgtgga gggttcgctc aacccaccgc tgaccaccac gatccgtcgg gtcatcgctt
     8581 gctctcctcg agctcggctt gatccgcagc agtgtcgtcg gacaccgctt ccgcggacac
     8641 cgtcttcgcc gcggccagga gggactggtg cgtcggcgcg tccggcacat gcgccgggcg
     8701 gaggccgtcg agctcgcgac gcagcaccgg cacgacgtcc gcaccgagca tgtccagctg
     8761 ctccagcacc gtcttgagcg gcaggccggc gtggtccatc aggaagagct gccgctgata
     8821 gtcgccgaag tgctcgcgga acgtgagggt cttctcgatg acctgctccg gacttccgac
     8881 ggtcaacggc gtctgctcca tgaactcctc cagcgccggg ccatgaccgt acaccggagc
     8941 gttgtcgaag tatggccgga actcacggat cgcgtcctgg gagttcttgc gcatgaagac
     9001 ctggccgccg aggccgacga tcgcctggtc ggccgagccg tgtccgtagt gctcgaagcg
     9061 agcccggtac aagccgatga gccgcatgaa gtgctccttc ggccagaaga tgtggttggc
     9121 gaagaacccg tcgccgtagt acgccgcctg ttcggcgatc tccggactgc ggatcgagcc
     9181 gtgccagacg aagggcggta cgccgtcgag cggccggggc gtcgacgtga aaccttggag
     9241 cggcgtacgg aaccggccct cccagtcgac cacgtcctct cgccagaggc gatgcagcag
     9301 gtggtagttc tcgatcgcga gcgggatgcc gtcccggatg tccttgccga accacggata
     9361 gacggggcct gtgttgccgc ggcccatcat caggtcgacg cgaccgtcgg cgaggtgctg
     9421 gagcttggcg tagtcttccg cgatcaggac agggtcggtc gtcgtgatca gggtcgtcgc
     9481 cgtcgacaag atgatgcgct cggtccgggc cgcgatgaac gccaacgtcg tggtcggcgc
     9541 ggaggcgatg aacggcgggt tgtggtgttc gccggtcgcg aacacgtcga gcccgacctc
     9601 ctcggccttc ttggcgatct cgatggtcgc cttgatgcgc tcgtactccg tcggcgtccg
     9661 gcccgtcgtg gggtcggtcg tcacgtcgcc gacgctgaag atcccgaact gcatgacgct
     9721 ccctgctctc gctcaagtag ttgacgaatc aatcatttga acgagggtcg atcgcgcggt
     9781 attcccgcgt tcccccgatg atcgcagaac gagcggtgtc gcacccctac ccggttgcgg
     9841 cccggacgcg cacactggat ggtggcagga gaccaaggat cgcggccagg ccgtgacagc
     9901 gtcagagccc gcatcgtgaa agggacagac tcgtgtttgc agcagtagga gaccgtctca
     9961 cggttcacag ccagcacctg gacggggtcg tgcgagacgg cgagatcatc gaggtccacg
    10021 gagaccaggg tcagccgccg tacctcgtgc gctggtccga cgacggtcac gaggcgctcg
    10081 tgttcccggg gccggatgcg tccgtcgagc acggtgagca ctccgcgaca gagtagtcgt
    10141 cgcggagccg cgggacacgt ccagcaggac gatcagtcca cagcgcgctc agcctgacgc
    10201 agaccgctgc tccacttctc ctcgatccga ccgaagcgcc agacagccag cgcggtcaac
    10261 caggtcacca cgaacagcgc gacgatcgcg tacccgacgt agttgaggtc gagggcggcc
    10321 acccacccga caacgccgtc ctcgagaccg aacttctccg agaacaccgc gacgagctcg
    10381 accatcccga tgatcagcgc gaccgcgacc gagagtgcgg tgaccgtgat gttgtagaag
    10441 accttccgga ccggttgggc gaacgcccac ccgtaggcga agttcatgaa cgcgccgtcg
    10501 atcgtgtcca gcaacgacat gccggctgca aacaggatcg gtaggcagag gatcgcgtac
    10561 cacgggagcc cgccggccgc tgcagccccc gccgtggcga gcagcgcgat ctcggtggcg
    10621 gtgtcgaagc cgaggccgaa gaggacaccg agcgggtaca tctgccacgg cttcttgatc
    10681 gcgttcgtcg cccggccgta gaagcggttc atgaagccgc ggttgttcag ctggtcctcg
    10741 agctcacgct cgtcgtacat gccgtgccgc atccggcgga acaccctgac gatgccgacc
    10801 agcaccagca ggttgaggat cccgatcagc agcaagaaca caccagagac actgggcccg
    10861 atgaccccgg tggccttgtg aaggctcgag ctgtcatcgg agacctcccc gctgagcccc
    10921 ctgatgcccc acgagaacag cagcgccagc acgaagacgt tcgtcgagtg cccgagggag
    10981 aagaagaatc cgacgctgag cgggcgctga ccgtcggtca tcagcttccg ggtggtgttg
    11041 tcgatcgccg cgatgtggtc ggcgtcgaac gcgtgacgca tgcccagcgt gtacgccgtg
    11101 atgccgacgc cgacgccgaa cagcgaaccg tcgccgaggc tgaagttgcc cggcacgacg
    11161 agggcgaaga gcagaccgaa gccgatcacg tggagcgcga ggatgaccgc gaacaagccg
    11221 gccgcgcgac gccactcggc cggcgtcaac gaggtgcgga agcggtgcca ccgcgaaccc
    11281 ggtggtggtg cgctggcggt cggagtccga gtcgttccgg tcgtcgtggt catgactgcc
    11341 tctcgggctg gtcgggcagg tggtctcctc ctttattcgg agcctaagcc cgatcggttc
    11401 gctgttgcaa ttcatacgca atagcaaccg agtagcagta gaccgcgatg tgcggggact
    11461 tccgggattc cgacgaccca gaacccggag gctcccgcac ttcgcgggcc gacaggttca
    11521 ggcggtcatc ggtgctgctc cgatgtcggc catgatcgcc tcgccggtcg cccgcgcatc
    11581 gcctccggcg aagacgaggg tgcgcgcgtg ttggtaccgg cacccggcgg tgctcagcgc
    11641 gtctgccgca gccaggagca tctcggtatc gtcggcgtcg atagccgcgg cccggtcgac
    11701 cagtgccgag gagatcgggt tctcgcggac gacgaacctg gcccggtcca gacggctccg
    11761 tcggtcaggc agggccgcca gcacaccggc ttcagcccaa accgcggcgt accacggtcg
    11821 ccacgcggcg tcgtgccatg gcttgaacga ctccggttcg cctgccacgt gcgtcagcgc
    11881 agccgcgatc tcaccccggt gcaacgccac catcccctcg aatgttgggc tgtagatggt
    11941 ccgccgtccg cgcatgtgtt ccaccacccg gctcatctcg gcggtgatgt cgagccagtc
    12001 ccgccgggcg tcgtcgtcgc ctcggatgcc gtacaccatg gctgccgcgg cgggagcgaa
    12061 ggcgatgcct ccgatggatg gccggccggc ctcctcccag gcgcgacgga agcgctgcgc
    12121 gagcgcgctg gcctcgtcga agtcgccggc aatcgccgcc gtggtgagca gccattcgac
    12181 cgcgagatgg tccgcctccc ggaagaagga cagctcgctg cgctcttgtg cgtggcggcg
    12241 ggcggtctcc aggtcgccgg cggcgacgta caccaggggc gccatgtgga gtgtgtcgga
    12301 gtactcccat gccagctcga cgtcgtgcgc acggggcgtg agcaggtcga ttcggcgtcg
    12361 gatggtggcc acggcgttgc ccacctcgcc ggagatgaag cgtgcggcgg tgagctggtc
    12421 cagcgcgtgg ctctccaggc ggacgtcgcc aacccgatgg gccaggtcgg cagcccgttc
    12481 ggccagctcg gcgtatcccg ggtcgacctc gtcgttcgga gtcgtgaccg tgagcacggc
    12541 cgcctcgaca tggagatcgc ctccggcgag ggctcgagcc tccgccagca gggcttgttc
    12601 ctcgcccggt ggagccagct cagacatgag gcccggcgcg ttcgtgatca gctcggccgc
    12661 acacaggagc tcgagcgccg cgcggtgtgg gtcgccggcc cggcgggcgg cttcggccgc
    12721 ggcccggtag agccgcatcg catcgttgcc caggtgacgg ccccaagcga cagccgcagc
    12781 gaggtgcagc gcgtgcgccg cgtcggcggg ttccggcgcc agcgcggccg cttcctcata
    12841 ccgctcctgt gcctcgctcg gcagcccact ggtataggcg agtcgtgcca ggagcagagc
    12901 aaggccatgg gcgtcggccc ggagttcggg ccggcacccg gcccagcgca gtccggcccg
    12961 gaggtcgtcg gccacttcct cgaacgcggc cgggggcccg ccgtcggcgg ccgcctgcga
    13021 agccgccgtc gccaggcacc agcgcaagtg ccggtctcgc acatcggtct cctcaccgac
    13081 gtccaccatg cgctcggtgc cgtactgccg gatggtctcc agcattcggt agtgagttcc
    13141 atcgagttcg gagctcacca caagcagact ctggtcagtg aggcgggcca acgacacggc
    13201 cacatccgaa ggcgacagcg gtgcgtgtcc ggccatcgcc accgcccgct cggacgtgaa
    13261 cggcgcggcg aacaccgacg tccgccgcag caccacctgg tccttctcgc cgagcaggcc
    13321 gaagctccag tccagggcgg agcggaccga ccggtgccgt gcgtcgaggc gaggcccacc
    13381 agcgagcagg tcgagccggt cggcaaggcc gatctccaag ccgtcgagcc ccaaggtcgt
    13441 caggcgggca gccgccagct cgatcgccag cgcaaccccg tcgagctgct cgcagatcgc
    13501 ggtgatgcgg cgttggtcgt agcggtatga cgacgaccaa cccgccatgg cggcccgatc
    13561 gacgaacaac gtcacggcgt cgctgtcgcc ttcgccggtg ccgtcatcga gtgacagccc
    13621 cggcacctgg aacaccgact cgaacggcac ctgcaaccgc acccggctgg tcgccagcac
    13681 ggtcacctcg ggacatgccg acagcagccg ttcgacgaac accgacaccc catccacgag
    13741 gtgctcgcag ttgtccagca ccaccagcgc ctcagccccg gccagcttc
//
LOCUS       DATBFZ010000015         2612 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_1977465, whole genome shotgun sequence.
ACCESSION   DATBFZ010000015 DATBFZ010000000
VERSION     DATBFZ010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1977465"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="VH419_00425"
     CDS             <1..390
                     /locus_tag="VH419_00425"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY7042111.1"
                     /translation="VAERAFEDALFYAKERKQFGKPIGSFQALQHRFADLAADLEAAR
                     LMTYWVASMVDQDEDRMLPKEASMVKLFVTETAKRVTLEGMQMMGGYGYSSEYDMERL
                     VRQTLVSTIYGGTSEIQRGIIAKTLGL"
     gene            759..>2612
                     /locus_tag="VH419_00430"
     CDS             759..>2612
                     /locus_tag="VH419_00430"
                     /inference="COORDINATES: protein motif:HMM:NF042648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N,N-dimethylformamidase beta subunit family
                     domain-containing protein"
                     /protein_id="HEY7042112.1"
                     /translation="MLLAGSPTAFGLLLFTVWGGSASAQVVDPCAPVQNPIVCENSKP
                     GTPQSEWDVNGGGDPSIEGFATDISVDHGETVHFKIDTPSTAYHLDIYRMGYYGGDGA
                     RKVATVTPSASLPQAQPTCDTDVPVGLVDCGNWSESAHWDVPADAVSGIYFAKLVRDD
                     DPSSEGSHILFIVRDDEGQSDLLFQTSDTTWQAYNRYGGNSLYSAEGEDGSPAGRAYK
                     VSYNRPFETRGPTPEDSPFNAEYPMVRFLERNGYDVSYFTGVDADRRGSEMLEHKTYL
                     SVGHDEYWSGTQRANVEAARAAGVNLAFFSGNEVFWKTRWENSIDGSGTDHRTLVCYK
                     ETHDGAKIDPGPPGNPNVWTGTWRDPRFSPPADGGRPENGLTGTIFTVNDGTSAIEVP
                     AADGKLRFWRNTSIAGLAPGQTATLSDSTLGYEWDEDLDNGARPAGLIDMSSTPVDVP
                     QRITDYGSNYGPGHATHSLTLYKAPSGALVFGAGTIQWSWGLDGDHDRGGSTPDSRMQ
                     QATVNLLADMGTQPDRLQAGLVTGTASTDTDPPTATISSPLADSNVESGVQTTISGTA
                     SDVGGGQVGSVEVSTDGGSTWHPAQGHEQWTYSWTPGTTGSATIKARAVDDS"
BASE COUNT          484 a          898 c          849 g          381 t
ORIGIN      
        1 gtcgccgagc gcgccttcga ggacgccctc ttctacgcca aggagcgcaa gcagttcggc
       61 aagccgatcg gctccttcca ggcgctccag caccgcttcg cagacctcgc cgccgacctc
      121 gaggccgccc ggctcatgac ctactgggtc gcgagcatgg tcgaccagga cgaggaccgc
      181 atgctcccca aggaggcgtc gatggtgaag ctcttcgtca ccgagaccgc caagcgcgtg
      241 accctggagg gcatgcagat gatgggcggc tacggctact cctcggagta cgacatggag
      301 cgcctggtcc gccagacgct ggtctccacc atctacggcg gcacctccga gatccagcgc
      361 gggatcatcg cgaagacgct cgggctctag ctgaggctga gaacccggca ccggcatcga
      421 cccggcgccg tggccatcca agggcggcag ccccaccagc gcgggcgatc aatactccag
      481 accgagttca atactgcgta gaggggttaa gtagaacacc gaaaatcggt atttacccct
      541 tgacaagctt cgcttcccgg cggttagcgt gatcacgcaa attgcgtgaa ctcctttgag
      601 gtggcagggc ctcgtggtag gcgcaaacat ccggtttgac cgcggctgtg tggccgtagg
      661 gagtggaagt gcagcgtgga gagggtcgat tcgggggccc ccggacggga cagccgggcc
      721 agtcctcagt cgccagggcg ctgcggcgag tcgcccacgt gctcctcgcc ggttcgccga
      781 cggcgttcgg gcttctcctg ttcacggtgt ggggtggctc ggcgtcggca caggtcgtcg
      841 acccttgcgc gccagtccag aacccgattg tgtgcgagaa ctcgaagccc ggcaccccgc
      901 agagcgagtg ggacgtgaac ggcggcggcg atcccagcat cgaggggttc gccaccgaca
      961 tcagcgttga tcacggcgag acggtccact tcaagatcga caccccatcg accgcctacc
     1021 acctcgacat ctaccggatg ggctactacg gcggcgacgg cgcccgcaag gtcgcgaccg
     1081 tcaccccctc ggcgagcctg ccacaggccc agcccacctg cgacacggat gtccccgtcg
     1141 gcctcgtcga ctgcggcaac tggagcgagt cggcccactg ggatgttccc gccgacgcgg
     1201 tctccgggat ctatttcgcg aagctcgttc gggacgacga ccccagctct gagggaagcc
     1261 acatcctctt catcgtgagg gatgacgaag ggcagtccga cctgctcttc cagacctccg
     1321 acacgacctg gcaggcgtac aaccggtacg ggggcaacag cctctactcc gcggaggggg
     1381 aggatggatc accggccggt cgcgcgtaca aggtcagcta caaccgtccc ttcgagacgc
     1441 gcggtccaac gcccgaggat tcaccgttca acgccgagta cccgatggtc cgcttcctcg
     1501 agcgcaacgg ttacgacgtc agctacttca cgggcgtcga cgctgatcgt cgcgggagtg
     1561 agatgctcga gcacaaaacg tacctctcgg tcgggcacga cgagtactgg tcgggcaccc
     1621 agagggccaa cgtcgaggcc gcccgcgccg ccggcgtgaa cctcgccttc ttctccggca
     1681 acgaggtctt ctggaagacc cgctgggaga acagcatcga cggctcgggc accgaccacc
     1741 gcaccctggt ttgttacaag gagacgcacg acggcgccaa gatcgacccc gggccgccgg
     1801 ggaacccgaa cgtctggacg ggaacctggc gcgatcctcg tttcagccct ccggctgacg
     1861 gaggccgtcc cgagaacggg ctgacgggaa ccatcttcac cgtcaacgac ggcaccagcg
     1921 cgatcgaggt gcccgcggcg gacggcaagc tccgcttctg gcgcaacacc agcatcgccg
     1981 gcctggcacc gggccagacc gccacgcttt cggacagcac actcggctac gagtgggatg
     2041 aggacctgga caacggggca cggcccgcgg ggctgatcga catgtcctcg accccggtgg
     2101 acgtgccgca gaggatcacc gactacgggt ccaactacgg ccccgggcac gcgacgcaca
     2161 gcctcactct ctataaggca cccagcggag cgctggtgtt cggcgcaggc accattcaat
     2221 ggtcctgggg cctcgatggg gaccacgacc gcggcggctc gactccggat tccaggatgc
     2281 agcaggcaac ggtgaacctg ctggccgaca tgggtaccca gccggacagg ctccaggccg
     2341 gtctggtcac gggcactgcc tccaccgaca ccgacccgcc gacggcgacg atctcgtccc
     2401 ccctcgcgga ctcgaacgtc gagtccggcg tgcagacgac catcagcgga accgcaagcg
     2461 atgtgggcgg tgggcaggtc ggatccgtgg aggtttcgac cgacggcggc agcacctggc
     2521 atccggccca gggccatgag cagtggacct acagctggac gcccggcaca accggcagcg
     2581 ccacgatcaa ggcgagggcc gtcgacgaca gc
//
LOCUS       DATBFZ010000016         4294 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_5255295, whole genome shotgun sequence.
ACCESSION   DATBFZ010000016 DATBFZ010000000
VERSION     DATBFZ010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 4294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4294
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5255295"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..740)
                     /locus_tag="VH419_00435"
     CDS             complement(<1..740)
                     /locus_tag="VH419_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014604479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEY7042113.1"
                     /translation="MPFSRYRAYTPVDLPDRTWPSKRIDHAPRWLSTDLRDGNQALID
                     PMTPARKLKMFELLVEMGYKEIEIGFPSASQTDFDFVRQLIEEDRIPDDVTVSVLTQA
                     REDLIERTAESLVGAKKATIHMYNATAPLFRRVVFRVDKAECLSIATHGTELVMKYAD
                     EHLGDVEFGYQYSPEIFTGTEPEFAVEVCNAVMDVWQPSSGREIILNLPATVEMSTPN
                     TYADLIEWFGRHVRNRDHVAISLHPHNDR"
     gene            1130..2137
                     /locus_tag="VH419_00440"
     CDS             1130..2137
                     /locus_tag="VH419_00440"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY7042114.1"
                     /translation="MPFVRVKPDDLTAVSTGVDLLNAAQRVDDPDGWPDIPELSAKWL
                     EFGWDLEPDERYLYVPVEGGEPVGVLDVSLPSRDNLHLVWAEVVVHPDHRRRGHGSAM
                     VAEVVRRAQDAGRTTIWLGTAEDDEGSRRFLEQQGFHYASHDARRRQILADVDGAEID
                     RLHATAQEAAADYELERYVPPIPDDVLAELVEVTEAINDAPMGELTYEDEKFDLQRLK
                     DLETARAGRGDRVYRLAARHRETGRFGGHTLVIVNPLRPTRAGQGDTAVHREHRGHRL
                     GMLLKIEMMRWLADVEPQVEVVETWNNADNRYMINVNEAIGYRLSRVFAMYERNLGTA
                     D"
     gene            complement(2314..3270)
                     /gene="era"
                     /locus_tag="VH419_00445"
     CDS             complement(2314..3270)
                     /gene="era"
                     /locus_tag="VH419_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017935636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEY7042115.1"
                     /translation="MSTEPVIEPASQESSFRSGFACFVGRPNAGKSTLTNALVGTKIA
                     IASSRPQTTRHAVRGIVHRPDGQLVLIDTPGLHKPRTLLGERLNDLVRATWTEVDVIG
                     VCLPANQRVGPGDTYLVGEIAALKRKPKLVAIATKSDLVSAGRMAEHLVAIGGLENNL
                     GIEWAHLVPVSATTGDQLPVLADVLLDLLPEGPPLYPDGEITDEPEEALVAELIRESA
                     LEGVHDELPHSITVVVEEMGLREGRPADKPLLDVYASMIVERDSQKGIVIGHRGSRLR
                     DIGAAARSQIETLLGTPVYLDLRVKVLKEWQRDPKHLKRLGF"
     gene            complement(3274..3624)
                     /locus_tag="VH419_00450"
     CDS             complement(3274..3624)
                     /locus_tag="VH419_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020574557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEY7042116.1"
                     /translation="MSESITPTNPEDRKIITLARASRARGGATQGACVRDGDGRTYAA
                     VNVALPSLNLSAIQLAVAMSVSSGAPGLEAVALVSDDEPAESDIAVIREVAGRPVAVW
                     VAAADGNVRSRHEV"
     gene            complement(3621..>4294)
                     /locus_tag="VH419_00455"
     CDS             complement(3621..>4294)
                     /locus_tag="VH419_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HEY7042117.1"
                     /translation="ILYLKDVIKRVYDSPDTQTTERVNSMMRRPVLCPDSKPVDALLK
                     EMQLKRVHLVIVVDEFGGTAGMATIEDILEEIVGEITDEYDEENTDVTELAEGRFRVS
                     PRLPLDELGELFGVELDDEDVETVGGVMAKLLNKIPIAGSTVSLAGIELVAERPSGRR
                     NKVGTVLATWIGDHDHDHDGATDARSIEAATQITRRAEEDRKRDDGERASQAGTDDKA
                     GRKAS"
BASE COUNT          678 a         1464 c         1425 g          727 t
ORIGIN      
        1 cggtcgttgt gcgggtgcag cgagatcgcc acatggtcgc ggttgcggac gtggcggccg
       61 aaccactcga tcaggtccgc gtaggtgttc ggcgtggaca tctcgacggt ggccggcagg
      121 ttgaggatga tctcgcgacc actcgaaggt tgccagacgt ccatcaccgc attgcatacc
      181 tcgacggcga actccggctc ggtcccggtg aagatctccg gtgagtactg gtagccgaac
      241 tcgacgtcgc ccaggtgctc gtcggcgtac ttcatcacca gctcggtgcc gtgggtggcg
      301 atcgacaggc actcggcctt gtccacccgg aagaccaccc gacggaacag gggtgccgtc
      361 gcgttgtaca tgtggatggt cgccttcttc gccccgacca gcgactcggc ggtccgctcg
      421 atcaggtcct cccgggcctg ggtcagcacc gagaccgtca cgtcgtcggg gatccggtcc
      481 tcctcgatga gctgccggac gaagtcgaag tcggtctggc tggccgacgg gaaaccgatc
      541 tcgatctcct tgtagcccat ctccaccagc agctcgaaca tcttcagctt gcgggccggc
      601 gtcatcgggt cgatcagtgc ctggttcccg tcacgcagat cggtgctcag ccatcgtggg
      661 gcatgatcga tccgcttgct cggccaggtg cggtcgggca ggtcgacggg ggtgtacgcg
      721 cggtagcgcg agaacggcat cgggcttggc tgctgcacgg gcacggcttg tggccgcgtg
      781 ccgaaactcg actgacccga tgacggtgtg tcgctggtgg tgctcatgaa gttcctcgct
      841 gctcggactg agtgcgggcc ggaacgcacg ccaagacgcc gcagcgagga tgccggccct
      901 tagtgggcct cgctgcggca gcgaaggagg agcagcgcaa acgcgcgctc ggcgctagcc
      961 atgccgctta ccttaaccca catcgccgcc tacgccaagg ctccctggtt tcacgacgct
     1021 cgactgctct catgggcccg attcgggctt ctcggcggtt cgagtgtcac gaaaccaggg
     1081 atctggaaaa ccagtggccc gggtgcgacg tctcaggcac cctgggtcaa tgcctttcgt
     1141 acgtgtcaag cccgacgatc tcaccgccgt gagcaccggg gtcgacctgc tcaacgccgc
     1201 gcagcgggtc gacgacccgg atggatggcc cgacatcccg gagctgtctg ccaagtggct
     1261 ggagttcggc tgggacctgg agccggacga gcgctacctc tacgtgccgg tcgagggagg
     1321 ggaaccggtc ggtgtgctgg acgtgagcct gccgagcagg gacaacctgc atctggtctg
     1381 ggccgaggtc gtcgtccatc ccgatcaccg tcgccgcggt cacggctcgg cgatggtcgc
     1441 cgaggtcgtg cgtcgcgcgc aagatgcggg tcggaccacg atctggctcg gcacggccga
     1501 ggacgacgag ggctcccgcc gcttcctgga gcagcagggc ttccactacg ccagccatga
     1561 cgcacgccgg cgccaaatcc tggccgacgt cgacggtgcc gagatcgatc ggctgcacgc
     1621 gaccgcccag gaggcggccg cggactacga gctggagcgc tacgtcccgc ccattcccga
     1681 cgacgtgctc gccgagctgg tcgaggtgac cgaggcgatc aacgacgccc cgatgggcga
     1741 gctcacctac gaggacgaga agttcgacct gcaacggctg aaggacctcg agacggcgcg
     1801 tgccggccgc ggcgaccggg tctatcggct cgcggcccgg caccgggaga cgggccgctt
     1861 cggtgggcac acgctggtca tcgtcaaccc gttgcggccg acccgcgccg ggcagggcga
     1921 caccgccgtt caccgcgagc atcgtggcca ccggctgggc atgctgctca agatcgagat
     1981 gatgcgctgg ctcgccgacg tcgaaccgca ggtcgaggtg gtcgagacct ggaacaacgc
     2041 cgacaaccgc tacatgatca acgtcaacga ggcgatcggc tatcggctgt cccgagtgtt
     2101 cgccatgtac gagcgcaact tggggacagc agactgatcg ctcaacgacg atgtccggat
     2161 cccgtcggcg ccaagtgcgc gtgctgacag cttgattccc cgcatgcgga gtgcgaagtc
     2221 gatcttcggg gtgcgaagtg ggccaaaccc aggcgcgcga cctagattcg ctcggccgac
     2281 gccgcgaaca cggcggtcgt gaaatcagtc cggtcagaag ccaagccgct tgaggtgctt
     2341 ggggtcgcgc tgccactcct tgagcacctt gacccgcagg tccagataga ccggcgtacc
     2401 gagcagcgtc tcgatctggg accgggcggc cgcgccgatg tcgcggagcc ggctgccccg
     2461 atgcccgatc acgatgccct tctgggagtc ccgctcgacg atcatcgagg catacacgtc
     2521 caagagcggt ttgtctgccg gccggccctc acgaagtccc atctcctcca ccacgacggt
     2581 gatcgagtgc ggcagctcgt cgtgaacccc ttccagagca gactcgcgaa tcagctcggc
     2641 gaccagagcc tcctccggct cgtcggtgat ctcgccgtcg ggatacagcg gcgggccctc
     2701 cggcagcagg tcgagcagca cgtcggcgag caccgggagc tgatcaccgg tcgtcgccga
     2761 gaccgggacc agatgagccc actcgatgcc gaggttgttc tccagtccac ctatcgccac
     2821 gagatgttcg gccatccgac cggcggacac caggtcggac ttggtggcga tcgcgacgag
     2881 cttgggtttc cgtttcaggg cggcgatctc gccgaccagg taggtgtcac cgggacccac
     2941 ccgctggttg gccgggaggc agacgccgat cacgtcgacc tcggtccagg tcgcccgcac
     3001 gaggtcgttc agccgctcgc cgagcagcgt gcgcggcttg tgcagcccgg gggtgtcgat
     3061 cagcacgagc tgaccgtcgg gacggtgcac gatgccgcgc acggcgtgcc gggtggtctg
     3121 cggcctggac gacgcgatcg cgatcttggt gcccaccagg gcgttggtca gcgtcgactt
     3181 gcctgcgttg gggcgcccga cgaagcacgc gaagcctgac cgaaaactac tctcctgcga
     3241 cgccggctcg atgaccggtt cggtgctcac cgctcagacc tcatgtcgac tccgcacgtt
     3301 cccgtccgct gcggccaccc aaacggcgac cggccgaccg gcgacctcac ggatcacagc
     3361 aatatcgctc tccgcgggct cgtcgtcgga gaccagcgcc accgcttcca gcccgggcgc
     3421 tccggacgag acgctcatcg cgacggcaag ctggatcgcg gacaggttga gggacggcag
     3481 cgccacattg acggcggcgt acgtccgccc gtccccgtcg cggacgcacg ctccctgcgt
     3541 ggccccaccg cgcgcccggg atgcccgagc cagcgtgatg atcttgcgat cctccgggtt
     3601 cgtgggagtg atcgattcgc tcacgacgcc tttcgtcctg ccttgtcgtc ggttccggcc
     3661 tgcgaggcgc gctcgccgtc gtcccgcttg cggtcctcct cggcccgccg ggtgatctgg
     3721 gtcgccgcct cgatgctgcg cgcgtcggtc gcgccatcat gatcgtgatc atgatcgccg
     3781 atccaggtcg ccagcacggt gccgaccttg ttccgccggc cgctcggccg ctcggccacc
     3841 agctcgatcc cggcgaggga gacggtcgaa ccggcgatcg ggatcttgtt gagcagcttg
     3901 gccatcaccc cgcccacggt ctccacgtcc tcgtcgtcca gctccacccc gaatagctca
     3961 ccgagctcgt cgagcggcag ccgcggcgac acccggaagc gtccctcggc cagctcggtg
     4021 acgtcggtgt tctcctcgtc gtactcgtcg gtgatctcgc cgacgatctc ctcgaggatg
     4081 tcctcgatcg tggccattcc ggccgttccg ccgaactcgt cgaccacgat caccaggtgc
     4141 acccgcttca gctgcatctc cttgagcaac gcatccaccg gcttggagtc cgggcagagt
     4201 accggtcggc gcatcatcga gttgacccgc tcggtggtct gcgtgtcggg tgagtcgtag
     4261 acacgcttga tcacgtcctt cagatagagg atgc
//
LOCUS       DATBFZ010000017         4349 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_3290779, whole genome shotgun sequence.
ACCESSION   DATBFZ010000017 DATBFZ010000000
VERSION     DATBFZ010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 4349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4349
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3290779"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="VH419_00460"
     CDS             <1..354
                     /locus_tag="VH419_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020578966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEY7042118.1"
                     /translation="AAGLTASAWKTSAGAASRVPVARATNLTRQLRDYQAAGLTVAGL
                     AADGEVDVADLAVATEPLVLVVGSEGKGLSRLVRETCDVLVRIPMTATTESLNAGVAA
                     GIALYEISRLRSAAR"
     gene            486..1643
                     /locus_tag="VH419_00465"
     CDS             486..1643
                     /locus_tag="VH419_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020391635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY7042119.1"
                     /translation="MPVDRLLPTDEAHDLMELVRDIASEQLAPRASEAEAAAEFPRDV
                     FTLLGKTGLLSLPFDEADGGGGQPYEVYLQVLEEIATAWVSVAVDISVHTLSTYALAQ
                     FGTDAQRHTLLPGMLSGDQLGAYALSEPQAGSDVSGIATKAVLDGDEYVVTGTKAWIS
                     HGPHADFWLLFARTGPDPRRGLSAFHVPVGTPGISFGAPEKKMGMTASTTTQVVFDRA
                     RVAADLRIGDEGQGMAIALSALDSGRLGIAACATGLAQAALDLAVSYAAERHQFGQPI
                     AEFQGLQFMLADMAAAVESARATYLMAARLRDAGRPFSRQASIAKLTASDTAMKVTTD
                     AVQVLGGYGYTRDFPVERYMREAKVMQIFEGTNQIQRMVVARSLLKDLQSR"
     gene            complement(1720..2934)
                     /locus_tag="VH419_00470"
     CDS             complement(1720..2934)
                     /locus_tag="VH419_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017932946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4032 domain-containing protein"
                     /protein_id="HEY7042120.1"
                     /translation="MALRVVATSQQPGLMTLPWDRPLEEWDDSVCVSLPRGISRHVVR
                     FVRLGHDVFAVKETREPIAWREYRLLRDLRRIGAPAVEAVGVVTGRQTPGGDEIEPCL
                     VTHHLTASMPYREMFSRGVRPGTMPRLVDALVVLLTRLHLSGFFWGDCSLSNTLFRRS
                     AGQFAAYLVDAETGELHHGLSNGQREYDIDTAEGNLFAEMLDLQAGGLVGEDEVDPYA
                     LIEVVRGRYETLWDELTGVEDFSTDEMWRIEQRIERLNQLGFDVDELDIVSDWDGATV
                     RIQPRVVDAGHHSRMLQGLTGLDVEDNQARRLLNDLNSYAAYHELQNADQSWVAHRWL
                     TEIYEPIIAMAPENRRGQLEAAEMFHEILEHRWYLSERAGEEVDLFETAKDYFAQQAN
                     PSAPLTELFVEP"
     gene            complement(2956..3426)
                     /gene="soxR"
                     /locus_tag="VH419_00475"
     CDS             complement(2956..3426)
                     /gene="soxR"
                     /locus_tag="VH419_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008359578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HEY7042121.1"
                     /translation="MARMPDHLSIGELSLRSGVAASALRFYEDVGLIEAERTAGNQRR
                     YPRHMLRRIAFIRAAQRVGLSLDEIAAALATLPDRRTPNKADWERVSRHWTARIDRQI
                     ADLQRLKTKLTSCVGCGCLSLRTCSLSNPDDEQGEAAAGPAWLATEENAGVTRD"
     gene            complement(3435..3608)
                     /locus_tag="VH419_00480"
     CDS             complement(3435..3608)
                     /locus_tag="VH419_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017935490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEY7042122.1"
                     /translation="MATDDKFENKAEEVKGQVKEKTGQVKGDDKMRAEGKAEQSKANL
                     KQAGENVKDAFKK"
     gene            3761..>4349
                     /locus_tag="VH419_00485"
     CDS             3761..>4349
                     /locus_tag="VH419_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015579712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEY7042123.1"
                     /translation="MSIANIERPPTYGYADLARLMVVQTGDEKHEEASTSTLDVLWVL
                     YDQVLRITPETVDDPHRDRFLLSKGHGPVAYYAVLAAKGFVPVDWLPQFGAFDSPLGF
                     HPDRNLVPGVEISSGSLGHGLPLAVGTTLGLRAQGITSRVVTLIGDGELDEGTNAEAI
                     VVAGVFGLDRLTVVAIDNSSDSLGWSGGLERRFDIE"
BASE COUNT          678 a         1489 c         1425 g          757 t
ORIGIN      
        1 gctgcgggcc tgaccgccag tgcctggaag acgtcggcgg gtgcggcgtc gcgggtaccg
       61 gtcgcgcgtg ctacgaacct gacacggcag ctgcgcgact accaggctgc aggcctgacg
      121 gtcgcgggtc tggccgccga cggcgaagtc gacgtggccg acttggcggt tgccaccgag
      181 ccgctggtgc tcgtggtcgg gagtgagggg aagggtctgt cgcggctcgt ccgggagacc
      241 tgcgacgtcc tcgtgcgtat ccccatgacg gccaccaccg agtccctcaa cgccggcgtc
      301 gccgccggca tcgccctcta cgagatctcc cgcctccgct ccgccgctcg ttgagtgctg
      361 ggttgcgggt tgttgagtgc tgggttgcgg gctctcgctg ctccattcga gtgggcgggg
      421 cattggacga gccgcggtta ggctgttggt gacccgcccg ccgaccgacg gagacgcgcc
      481 gcagcatgcc agttgaccgc ctgcttccca ctgacgaggc tcacgacctg atggagctcg
      541 tgcgcgacat cgcctccgaa cagctcgccc cacgcgcctc agaagccgag gcggcggccg
      601 agttcccgcg tgatgtgttc acgctgctcg gcaagacagg tctgctctcg ttgccgttcg
      661 acgaggccga cgggggcggg ggccagccct atgaggtcta cctgcaggtc ctggaggaga
      721 ttgcgaccgc ctgggtgagc gtggcggtcg acatctcggt ccacaccttg tcgacgtacg
      781 cgctggccca gttcggcacg gacgctcaac ggcatacctt gctgccgggg atgttgagcg
      841 gcgaccagct cggcgcctat gccttgtcgg agccgcaagc cggatcggac gtgtcgggca
      901 tcgccaccaa ggcggtgctc gacggtgacg aatacgtggt cacgggcacc aaggcgtgga
      961 tcagccacgg gccgcacgcc gacttctggc tgctgttcgc gcggacgggg ccagaccccc
     1021 gacgtggtct gtcggcgttc catgtccccg ttggtacgcc gggtatctcc ttcggcgcgc
     1081 ccgagaagaa gatgggtatg accgcgtcga cgacgacgca ggtggtcttc gacagagcac
     1141 gggtggcggc ggatctgcgg atcggcgacg agggacaggg gatggcgatc gcgctctcgg
     1201 cgctcgactc cggtcggctc ggcatcgctg cctgcgccac cggtcttgcg caggcggcgc
     1261 tcgaccttgc agtctcgtac gccgccgagc gtcaccagtt cggccagccg atcgcggagt
     1321 tccagggact gcagttcatg ctcgccgaca tggcggccgc cgtcgagtcc gcccgggcga
     1381 cgtacctcat ggccgcgcgc ctgcgcgatg ccggtcgacc gttcagtcgg caggcatcga
     1441 tcgcgaagct gaccgcctcc gatacggcga tgaaggtcac cacagatgcc gtgcaggtac
     1501 tcggcgggta tggctacacg cgcgatttcc ccgtggagcg gtacatgcgg gaggcgaagg
     1561 tgatgcagat cttcgagggg acgaaccaaa tccagcggat ggtcgtcgcg cgttcgcttc
     1621 tgaaggatct gcaatctcgc tgacaaccac aagtcacggc tcaacggacc acaactcacg
     1681 gttcaacgga ccacaactca cggttcgatg aaccacaact cacggttcaa cgaagagttc
     1741 ggtgaggggg gctgaagggt tggcttgctg ggcgaagtag tccttggccg tctcgaagag
     1801 atcgacctct tcgcctgcgc gctccgacag gtaccagcga tgctcgagga tctcgtggaa
     1861 catctcggcc gcctcgagct gccctctccg gttctccggc gccatggcga tgatcggctc
     1921 gtagatctcc gtcagccagc gatgcgcgac ccaggactgg tcggcgttct gcagctcgtg
     1981 gtacgccgcg taggagttca ggtcgttgag cagacgacgc gcctggttgt cctcgacgtc
     2041 cagcccggtc agtccctgca acatccgcga gtggtgtccg gcgtcgacca ccctcggctg
     2101 gatccgcacg gtggcgccgt cccagtcgga gacgatgtcg agctcgtcga cgtcgaagcc
     2161 gagctggttc agccgctcga tccgctgctc gatccgccac atctcgtcgg tgctgaagtc
     2221 ctccacacca gtgagctcgt cccacagcgt ctcgtaccgc ccccgcacga cctcgatcag
     2281 cgcgtacgga tcgacctcgt cctcaccgac cagcccacca gcctgcaggt cgagcatctc
     2341 cgcgaacagg ttgccctccg cggtgtcgat gtcgtactca cgctggccgt tgctcagccc
     2401 gtggtgcagc tcgccggtct ccgcgtcgac gaggtacgcc gcgaactgcc cggccgagcg
     2461 acggaagagt gtgttcgaca acgaacagtc gccccagaag aacccgctca ggtgcagcct
     2521 cgtcaacagc accaccaacg cgtcgacgag gcgcggcatc gtcccaggtc ggactccgcg
     2581 cgagaacatc tcgcggtacg gcatcgacgc ggtcagatga tgggtcacca gacacggttc
     2641 gatctcgtca ccacctggtg tctgccgtcc cgtcacgacc cccacggctt cgaccgccgg
     2701 tgctccgatg cgccgcagat cccgcaacag tcggtactcc cgccaggcga tcggctcacg
     2761 cgtctccttg acggcgaaca cgtcgtgccc gagccgcacg aaccgcacca cgtggcggga
     2821 gatcccacgc ggcaatgaca cgcagaccga gtcgtcccac tcctcgagcg ggcgatccca
     2881 ggggagcgtc atcagccctg gctgctgact ggtggcgacg acgcgcaagg ccatacagac
     2941 gaccctagtg cggcgttagt cccgggtgac gccggcgttc tcctcggtgg cgagccaggc
     3001 ggggccggcg gccgcctcgc cctgctcgtc gtcggggttg ctcagggagc aggtccgcag
     3061 gctcagacat ccgcatccga cgcagctggt gagcttggtc ttgaggcgtt gcagatcggc
     3121 gatctgccgg tcgatccgcg ccgtccagtg gcgtgacacc cgctcccagt cggccttgtt
     3181 cggcgtacgc cggtcaggca gcgtcgcgag cgccgcggcg atctcgtcga ggctgagccc
     3241 aacccgctgt gcggcccgga tgaacgcgat ccgacgcagc atgtgacgcg ggtagcggcg
     3301 ctggttgccg gccgtgcgct cggcttcgat cagcccgaca tcctcgtaga agcgcaacgc
     3361 ggatgccgcc acccccgagc gcaacgacag ctcgccgatg ctcaggtggt cgggcatccg
     3421 cgccatgcat gctcctactt cttgaacgcg tccttcacgt tctcaccggc ctgcttgagg
     3481 ttggccttgg actgctcggc cttgccctcg gcgcgcatct tgtcgtcgcc cttgacctgg
     3541 ccggtcttct ccttgacctg tcccttgacc tcttcggcct tgttctcgaa cttgtcgtcc
     3601 gtcgccatct ccgcacctcc gtcagctgga acggccggta ggcggttcgc ccccagcacc
     3661 tcgtacaaag gcgacgtacc caacaaacgg tgacaccatg ccatgctccg caggcaaagg
     3721 ctcttgactt aaagtttact tgaagtcgca ggcttcctcc atgtccatcg caaacatcga
     3781 acgcccgccg acctacgggt acgccgacct ggcccggctg atggtggtcc agaccggaga
     3841 cgagaagcac gaagaggcgt ccacatcgac gctcgacgtg ctctgggtcc tctacgacca
     3901 ggtcctgcgg atcacgcccg agacggtgga cgacccgcac cgggaccggt tcctcctcag
     3961 caagggacac gggccggtgg cctactacgc cgtgctcgca gccaagggct tcgtgccggt
     4021 cgactggctg ccacagttcg gcgcgttcga ctcgccgctc ggctttcacc cggatcgcaa
     4081 cctggtccca ggggtcgaga tctcatccgg ctcgctcggt cacgggctgc cgctggcggt
     4141 cgggacgacg ctcggcctcc gtgcccaggg catcacgtcc cgcgtggtga cgctgatcgg
     4201 ggacggggag ctcgacgagg ggacgaacgc cgaggccatc gtggtcgccg gtgtcttcgg
     4261 actcgaccga ctcaccgtgg tcgccatcga caactcgtcg gactcgttgg gctggtcggg
     4321 tggactcgag cgccgtttcg acatcgagg
//
LOCUS       DATBFZ010000018         1510 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_2963385, whole genome shotgun sequence.
ACCESSION   DATBFZ010000018 DATBFZ010000000
VERSION     DATBFZ010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2963385"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            493..618
                     /locus_tag="VH419_00490"
     CDS             493..618
                     /locus_tag="VH419_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042124.1"
                     /translation="MNEAVIEARAGYLIEDRINTTQRRSSRLRRHHRHLRTSSWL"
     gene            complement(696..1217)
                     /locus_tag="VH419_00495"
     CDS             complement(696..1217)
                     /locus_tag="VH419_00495"
                     /inference="COORDINATES: protein motif:HMM:NF026766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm26 family immunity protein"
                     /protein_id="HEY7042125.1"
                     /translation="MARKKYCKPGDVFTVPLGDGRVGIGQVAAGYAPMSAYYMTIFDV
                     VTREEDAPSDVAAALAAPVLFASLSYDGKIYAGHWRIIDHAPVSRTTRLPAYRVAIGA
                     PPRPYIEDFFAIKRRRASRKEARALPERGFVSPAVLERALRAHHGMEPWLPAFDDLAL
                     NQLAIADRFATDA"
BASE COUNT          268 a          493 c          499 g          250 t
ORIGIN      
        1 ccagcgcatg caaccgaacg agcacgtcgg ccttgtcatc ggtcgtcaga aacgcggggt
       61 cgacccctgc gacgtccttc agggcggcct cgacggcatc tgcgcacgcc acgagagggt
      121 gcaccgccat ccgctcaccc acccggcacc tcctgcgatc gacttctgcg cgcaagctct
      181 cagtcgatgg cagcgtgccc agaggcgggc caccaccacg gtggcccgtc cggagcctca
      241 gtcatcacta tcactccgga gtggcactat tcgaacacac gttcgatcat gtgtcaagga
      301 gcctgaagtg actggtgatg tctcaggaca tgggtgacag ttctgcgtcg tcctggtgca
      361 gcgatttgcc gaggtcgccc gacgagtcct tcccgttgtc gaaacgaagc gaagggtgtt
      421 cgtcgaacca cggacggggc gcccacgggg atggcgcccc ccgagaggac gaaaccgaca
      481 aggagtacca cgatgaacga ggccgtcatc gaggcccggg ccggctatct gatcgaggac
      541 cggatcaaca ccacacagcg gcggtcctcc cgtcttcggc gtcaccatcg gcacttgcgc
      601 acctcgagct ggctgtgacg tcgcgtggtc agacgttccc gacccggcgg aagaggcgcc
      661 tctccgcggc gcgtgagtgc gttcgctgcg gccgctcagg cgtcggtggc gaagcgatcg
      721 gcgatggcca gttgattgag cgcgaggtcg tcgaaggccg ggagccaggg ctccattccg
      781 tgatgggcgc gcagcgccct ctccaacacg gccggcgaca cgaagcctcg ctctggcaac
      841 gcacgagctt ccttgcgtga ggctcgccgc cgtttgatgg cgaagaagtc ctcgatgtac
      901 gggcgcggcg gagccccgat cgccacccgg taggcgggca agcgtgtggt tcgactcacg
      961 ggtgcgtggt cgatgatcct ccagtgcccg gcgtagatct tgccgtcgta cgagagagac
     1021 gcgaacagga cgggtgcagc gagggcagcc gctacgtccg acggcgcatc ttcttctcgt
     1081 gtgacaacgt cgaagatcgt catgtagtag gcggacatag gtgcgtaccc ggcggccacc
     1141 tgcccgatcc caacgcgacc atcgccgagg ggaacggtga acacatcacc gggcttgcag
     1201 tacttctttc tcgccacact ggcctcccgg acgtcccagt tcgagcacat cctgccgggt
     1261 cggagaccgt gccgctgagg attgacccga ctttcgctca tcggcagaac cgctcatgtc
     1321 agcggatgag cgggcgcttc ggcggcgacg ggtcagcacc agccggtcta ggagacagga
     1381 ggaggaggag accgtcagtt gagcatcccc gacgcgtcac ctgccgccct tccaccaccc
     1441 tttgtcggcg atgctgtgtc cgccggggtt cgcgacgcga cggggccagt tgcggaacag
     1501 gttggagcga
//
LOCUS       DATBFZ010000019         3140 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_2964734, whole genome shotgun sequence.
ACCESSION   DATBFZ010000019 DATBFZ010000000
VERSION     DATBFZ010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 3140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3140
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2964734"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1657)
                     /locus_tag="VH419_00500"
     CDS             complement(<1..1657)
                     /locus_tag="VH419_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013160627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEY7042126.1"
                     /translation="MPDDTSVTPLTKVLIANRGEIAVRVIRACKDAGIGSVAVYAEPD
                     RDALFVRLADEAYSLEGSTPADSYLDIDKIIAAAKRSEADSVHPGYGFLAENATFARS
                     VIDAGLVWIGPPPPAIDALGDKVQARHIAQKVGAPLVAGTSDPVADADEVVAFARENG
                     LPIAIKAAFGGGGRGLKVARTEDEIVELYDSAVREAVTAFGRGECFVERYLDKPRHVE
                     TQCLADQHGNVVVVSTRDCSLQRRHQKLVEEAPAPFLSDDQMKTLYSASKAILEEAGY
                     VGAGTCEFLVGQDGTISFLEVNTRLQVEHPVTEEVTGIDLVREMFRVAAGEELGYDDP
                     VVEGHSIEFRINAEDAGRNFMPAPGTLTRWHAPSGPGIRLDGGYDQGETVPGSFDSLV
                     AKLIVTGRDREQALERSRRALDEFVVEGMPTVIPFHRAVVDDPAYIGDGHTFGVYTSW
                     IENEFDNTIAPYDGETDVAEVGERETVIVEVGGKRLQVSLPAGLAAGATTAAAKKPTK
                     RSAGKKAGAAAGGNALTAPMQGTIVKLAVEEGQQVEAGELVVVL"
     gene            complement(1726..2337)
                     /locus_tag="VH419_00505"
     CDS             complement(1726..2337)
                     /locus_tag="VH419_00505"
                     /inference="COORDINATES: protein motif:HMM:NF024898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY7042127.1"
                     /translation="MVRPRFEARPLTPSTWGDLEDLFALPGGSIVRGCWCMFYRRTGP
                     VGVSAAAGVTNKQQLCSLVDGGVVPGLVGYLDGNPVGWISLGPREDYLKLRRSSVMKP
                     VDDTEVWSVVCTYVAKSHRGEGIQHKLLAHAIDWARDQGVRTLEAYPVDKPERSHDDF
                     MFFGSRSLYERAGFREVVRRSPTRVVMRRGLRPRRTSSRGGRH"
     gene            complement(2347..2778)
                     /locus_tag="VH419_00510"
     CDS             complement(2347..2778)
                     /locus_tag="VH419_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012919074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY7042128.1"
                     /translation="MAAHPSTARDEPTWTITELAAEYDVTLRTLRHYEDVGLLSPERQ
                     GTKRIFHNRDRIRLALILRGRRIGFSLDEIATIVNMYDEQPGEAGQLVYLLEQIEVRR
                     CELAQRRRDIDETLVELDQVEARCREDLVALQHGEPSPAGA"
     gene            2886..>3140
                     /locus_tag="VH419_00515"
     CDS             2886..>3140
                     /locus_tag="VH419_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008363359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY7042129.1"
                     /translation="MAPFELSPEHEDFRQSIREFAQAEIAPYAAKWDKDHHFPVDTVQ
                     QMGKLGLFGLTAPEEYGGAGEDGDFTSLCVAIEEIGRVDQS"
BASE COUNT          477 a         1046 c         1081 g          536 t
ORIGIN      
        1 cgagtacgac gacgagttct ccggcctcga cctgctgacc ctcctccacc gcgagcttga
       61 cgatggtgcc ctgcatggga gcggtgagcg cgttcccgcc tgccgcggcg cccgccttct
      121 tgcccgccga gcgcttcgtc ggcttcttgg ctgcggccgt ggttgctccg gccgcgagcc
      181 cggccgggag ggagacctgg agtcgcttcc cgccgacctc gacgatcacc gtctctcgct
      241 caccgacctc ggcgacatca gtttccccgt cgtatggcgc gatcgtgttg tcgaactcgt
      301 tctcgatcca gctggtgtag acgccgaagg tgtggccgtc gccgatgtac gccgggtcgt
      361 cgacgacggc gcgatggaac gggatcaccg tcggcatacc ctccacgacg aactcgtcga
      421 gcgcgcgacg cgaccgttcg agcgcctgtt cacggtctcg gccggtgacg atgagcttcg
      481 ctaccagcga gtcgaatgat cccggcaccg tctcgccctg gtcgtagcca ccgtcgagcc
      541 tgatccccgg gccggacggc gcgtgccatc gcgtcagggt gccgggtgcc ggcatgaagt
      601 tgcggcctgc gtcttcggcg ttgatccgga actcgatcga gtgcccttcc acgaccgggt
      661 cgtcgtaccc gagctcctca ccggcggcga cgcggaacat ctcgcggacg aggtcgatgc
      721 cggtgacctc ttcggtgacc gggtgttcga cctggagccg ggtgttgacc tcgaggaacg
      781 agatcgtgcc gtcctggccg acgagaaact cgcaggtccc ggcaccgacg tacccggcct
      841 cttcgaggat cgccttggag gcggagtaca gggtcttcat ctggtcgtcg gagaggaagg
      901 gcgcgggcgc ctcctcgacc agtttctggt ggcggcgttg cagcgagcag tcgcgggtgg
      961 agaccacgac gacgttcccg tgctggtccg cgaggcactg cgtctcgacg tgtcgcggct
     1021 tgtcgaggta ccgctccacg aagcactcac ctcgcccgaa cgccgtcacg gcctcgcgga
     1081 cggcagagtc gtacagctcg acgatctcgt cctctgttcg cgcgaccttg aggccgcggc
     1141 cgccgccgcc gaacgccgcc ttgatcgcga tcggcaggcc gttctcgcgg gcgaacgcca
     1201 cgacctcgtc ggcgtccgcc accggatctg atgtgccggc cacgagcggt gcaccgacct
     1261 tctgggcgat gtggcgtgcc tggaccttgt cgcccagcgc gtcgatcgcc ggaggcgggg
     1321 gtccgatcca gacgagcccg gcgtcgatga ccgatcgtgc gaacgtggcg ttctcggcga
     1381 ggaagccgta gccggggtgc accgagtcgg cctcgctgcg cttggcggcg gcgatgatct
     1441 tgtcgatgtc gaggtaggag tccgcggggg tcgagccttc cagtgagtag gcctcgtcgg
     1501 cgagcctgac gaacagcgcg tcccggtccg gctcggcgta caccgcgacg ctgccgatgc
     1561 ctgcgtcctt gcacgctctg atgacccgga cggcgatctc cccacggttg gcgatcaaga
     1621 ccttggtcaa cggggtgacg ctcgtgtcgt caggcacgag gctgagtcta ggaccggtca
     1681 gcccgtgggc gcccttgggg agagccgatc cccgagcgcc ggggctcagt gccgcccccc
     1741 tcgggacgac gtacgccgcg gacgaaggcc acggcgcatc acgacgcggg tcggtgaccg
     1801 gcggacgacc tcgcggaagc cggcccgttc gtagaggctg cgtgacccga agaacatgaa
     1861 gtcgtcgtgg ctgcgttctg gcttgtcgac ggggtacgcc tccagggtgc ggacaccctg
     1921 gtcgcgcgcc cagtcgatgg cgtgggcaag cagcttgtgt tggatgcctt cacctcgatg
     1981 cgacttggcg acatacgtgc agacgaccga ccagacctcg gtgtcgtcga ccggcttcat
     2041 gaccgacgac cgtcgcagct tcaggtagtc ctcgcggggg ccgaggctga tccagccgac
     2101 cgggttgccg tcgagatagc cgaccaggcc agggacgacg ccgccgtcga cgaggctgca
     2161 gagctgttgc ttgttcgtga ccccggcggc cgccgacacc ccgaccgggc cggtcctgcg
     2221 gtagaacatg caccagcagc cacgcacgat cgacccgccc ggcagggcga acagatcctc
     2281 gagatcaccc caagtgctcg gtgtgagagg tcgcgcctcg aaccgtggcc gcaccatgga
     2341 ggtcagtcaa gcaccggccg gcgacggttc gccgtgttgc agcgcaacga ggtcctctcg
     2401 gcagcgcgcc tcgacctggt cgagctcgac cagggtctcg tcgatgtcgc ggcggcgttg
     2461 ggcgagctcg cagcggcgta cctcgatctg ttcgagcagg tagaccagtt ggcctgcttc
     2521 tcccggctgc tcgtcgtaca tgttgacgat ggtggcgatc tcgtccaggg agaacccgat
     2581 gcggcggcca cgcaagatca aggcaagccg gatccggtcc cggttgtgga agatccgctt
     2641 ggtgccctga cgctccggtg agagcagccc gacgtcctcg tagtgccgca gggtgcgaag
     2701 cgtgacgtcg tactccgctg ccagctcggt gatggtccag gtcggttcgt cgcgagcggt
     2761 gctcgggtgc gctgccacct cttgcccttc cgttacggaa gcgatctatc gtggacgacc
     2821 aagtttacgt ttacgtaaga gtaagcagac agaccagcgc gccgtcaaca tgcaggagaa
     2881 ccgccgtggc accgttcgag ctgtcccccg aacacgagga cttccgtcag agcattcgtg
     2941 agttcgcgca ggcggagatc gcgccgtacg ccgcgaagtg ggacaaggac caccacttcc
     3001 ccgtcgacac cgtccagcag atgggcaagc tcggcctgtt cgggctgacc gcgccggagg
     3061 agtacggcgg ggccggtgag gatggcgact tcaccagcct gtgcgtcgcg atcgaggaga
     3121 tcggccgggt cgaccagtcg
//
LOCUS       DATBFZ010000020         6924 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_1651623, whole genome shotgun sequence.
ACCESSION   DATBFZ010000020 DATBFZ010000000
VERSION     DATBFZ010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 6924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6924
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1651623"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="VH419_00520"
     CDS             complement(<1..237)
                     /locus_tag="VH419_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042130.1"
                     /translation="MSSNWGPARPDLQVDVGDTEPSPWAGRVLTFAGALMLVAGIFEL
                     FEGIQALANDSFFNLRGDYTYDMSVTAWGWIHLVL"
     gene            465..719
                     /locus_tag="VH419_00525"
     CDS             465..719
                     /locus_tag="VH419_00525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042131.1"
                     /translation="MDHYVIRVDGALPDNALQDFANVSVTAGSVQTVLHGDLPDQSSL
                     AGILDYLDALGVEIVEVLKVPRERRSVSDEAEIAGDPEGS"
     gene            complement(678..3488)
                     /locus_tag="VH419_00530"
     CDS             complement(678..3488)
                     /locus_tag="VH419_00530"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEY7042132.1"
                     /translation="MPRKTTTTGRSAVPSVGLRRAPRTPALLVPRPRLFKLLDSGTRK
                     PLTLVIGPAGTGKTVLLSSWLEQSRSTRLRRARWNSPHDGISVEELLLIAAGVDAEDA
                     AALTAGKPGVASPRVHDALVQRFDDGHRPDLLIVDDAHLLPPSTVALLARVLTTRPEA
                     VNLLIGSRRDLAVPVLELELKDAATTVRAGQLRFTPDESERLVRQHSSHVSPAAIVEL
                     ARRADGWAAALVLGAHAVAGVSEPEAIRSALGGTDQPVLDFLLGEAFAALSDGVKELL
                     LSTFEEPELTGLRAVVLTGRSDAGALLSDLAADGLLVTAYAGQSTSEMVYSYHPLLVE
                     LLRRRITADSGDVSTVADAHLRAARYDAAQGKRASALRHAIASHEPQMVAELLVEHGP
                     ALLTRGDLEVVAQAQDELGYDAMTNFPFLRGIVGLYRRAVGDVGGAVVLAAHAQETGA
                     TMPAETVTESLLRDAVRADAMILQLWQTHLGWQDASAVIAEARRVLGCLQSSTHAHRP
                     VLVLSPERQAWLLLELASAEMWAGDLDSAAVHVDEALLSARASDHPGLLALGLGHRAV
                     GELLKSNARGAADTAAEGLTHAQRTGSADRCFTDRSHITLAWASYLRLEFDQAFESLS
                     RIDLDAPGQVDTTVSILSSVLHAALLLEQGRLDEAQRELADPVGAAAALPSYLDRALA
                     LTRWRCASARGDGDAAAAEVRVLTKLGFDGDADVLSALARAELGDVDAGLETLSRVLT
                     AAGPTDQASLARAATARTALLLLNGDRNAAQIAVLDVLARTAPQELLFFLTIGLLAGR
                     DFLDLLADETKRAGAHPFAARALRSLREYVANRPHAIAASSPEIARAERRVRSETVDR
                     LQPKGIRNRAVVVNGVNVTLTEREIDVLEQLALGSSYHEIGLALFITENTVKTHLASL
                     YRKLGVERRSAALRVARDLGLV"
     gene            complement(3606..4718)
                     /locus_tag="VH419_00535"
     CDS             complement(3606..4718)
                     /locus_tag="VH419_00535"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEY7042133.1"
                     /translation="MSDVAGRPPGLSNGLPRTVVLLLGLAAAVVVVGGLRSASFIVSD
                     VFLALVLTIAVYPAQTYVIRRGWPRWSAFLVGALGVFVILIAIVGSVVLALARFASLL
                     PSYQDEAQQRIEDVTNRLDQLGIHTEQQQSLASSIDPARLVGYVGDLLGELASISSSL
                     FFILTVLFFMAIDSYQFPTLLEFARNRHPQMVDSLISFARGTRTYLVVSSGFGFIVAV
                     IDVIALALLGIPAPFAWGVLSFVTNYIPNIGFVVGLVPPAALAYLEGGPGLALAVIAA
                     YSVINFVIQSLIQPKIVGNAVGLTPTITMVSLVFWAWVLGALGALLAIPLSLLAKAVL
                     VDADRDAGWLQPLISGTVDQTTRFAPGDSGGVSDKS"
     gene            complement(4715..4933)
                     /locus_tag="VH419_00540"
     CDS             complement(4715..4933)
                     /locus_tag="VH419_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042134.1"
                     /translation="MTYYIIRVEGQLSAGLMATFPHLEATQHAQTVLHGHLDKQSELS
                     EVLGYLGEIGVDVVEVHRLPENEDVMPV"
     gene            5109..6035
                     /locus_tag="VH419_00545"
     CDS             5109..6035
                     /locus_tag="VH419_00545"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HEY7042135.1"
                     /translation="MSGDDANGTQSEARLRSRLARAIDRSRRVALFLVDVVPGLRRLL
                     AEVARNEIIDRSLVVAAQAMFSVTPLLIVVAAFSPRTFGDSLSESMANVMGVQQEDTT
                     ALQRAATVDQVRAQTGIVGVAIVLVSALSFARALQRMYERVWQQQHRGGVVGNRRCFF
                     WLVSWIAYLEMIAVLSHVLPRGDLSTLRLVVQFVVSTAVWWWTMRMLLLGRVGWPNLL
                     LGALLTSIGVLLLTRFSRLLMPAYVGSAVDQFGTLGVIFAISTWLLVFGGILVVAAII
                     GRVYTEELTIMQRFIRMFAKRTAPDSQPAGGA"
     gene            6035..>6924
                     /locus_tag="VH419_00550"
     CDS             6035..>6924
                     /locus_tag="VH419_00550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042136.1"
                     /translation="MDDDTTANGPDEDRPPGSDEELKAEVAALRAELATRPAGGRSSK
                     GWWRPLVCGLLLALVVVLSPLSVLAVWTHAEIADTDRYVDTVAPLASNPDVQDAVANR
                     ISTTLLTYIDVPKLTSETVEALSARGLSDRAAAALTALSVPLTNAVTDFVHTKVLELV
                     QSDAFAQAWEEANRQAHEQMVAVLTGETNGAVSVSGNEVSVKLATFIDTVKQALVDQG
                     FQLASRIPTVDATFVVFSSDNLGKAQQGFHLLDTAAIVLPLLTLVLIVVAVFVARVRR
                     TAIIAAGLCVAGGMLLLGLAL"
BASE COUNT         1155 a         2254 c         2394 g         1121 t
ORIGIN      
        1 gagcaccagg tggatccagc cccaggcggt cacgctcatg tcgtaggtgt agtcaccgcg
       61 gaggttgaag aacgagtcgt tcgccagtgc ctggatgccc tcgaacagct cgaagatccc
      121 agcgaccaac atcaacgcgc cggcgaacgt gaggactcga ccggcccacg ggctcggctc
      181 ggtgtcaccg acgtctactt ggaggtcggg acgtgctggt ccccagttgc tcgacatcgt
      241 cgactcctcg gcaagacagg ctgcggacag actgcggaca ggctgcggac accgccatcg
      301 tcgaccgcgc cgggcctgca tcgcctcccc cgataagggt gaacgtcgga ggctgacctg
      361 cgacgcctgg cccggtcgcc cgtcgtgggt gaatcgcgca gcatccgcgt cgctcacact
      421 cgaacctgaa cgagggctgc gcgagtggct ggtgagggtg ccggatggac cactacgtca
      481 tcagagtcga tggtgcgttg ccagacaacg ctctgcagga ctttgccaac gtgtccgtga
      541 ccgctggctc ggtgcagacc gtgctgcacg gagacctgcc ggaccagtcg tcgcttgccg
      601 gcatcctcga ctatctcgac gcgctcgggg tcgagatcgt cgaagtcctg aaggtccctc
      661 gggagcggcg gtccgtgtca gacgaggccg agatcgcggg cgacccggag ggcagctgac
      721 cgacgctcca cgccgagctt gcggtacagc gacgcgaggt gggtcttgac ggtgttctcc
      781 gtgatgaaca gcgcgagccc gatctcgtgg tacgagctgc ccagcgcgag ctgttcgagg
      841 acgtcgatct cgcgttcggt cagcgtgacg ttcacaccgt tgacgacgac ggcgcggttg
      901 cgaatccctt tcggctgcag ccggtcgacg gtttccgagc gcacgcgacg ttcggcacgg
      961 gcgatctccg gcgacgaggc ggcgatcgcg tgcgggcggt ttgcgacgta ctccctgaga
     1021 cttctcagtg ccctggcggc gaagggatgc gcccctgccc gtttggtctc gtcggccagc
     1081 aggtccagga agtcacggcc cgccaacagc ccgatcgtca ggaagaacag cagttcctgc
     1141 ggcgctgtcc tcgccaggac gtcgagaacg gcgatctggg ccgcgttccg gtcgccgttc
     1201 agcaacagga gcgctgtgcg agccgtggct gcgcgcgcga gcgacgcctg gtcggtcgga
     1261 ccggcggccg tgaggacgcg actcagcgtc tcgaggccgg cgtcgacgtc gcccagctcc
     1321 gcacgggcca gggccgacag gacatcggcg tcaccatcga agccgagctt ggtgaggacg
     1381 cgcacctcag cggcggcggc gtcgccgtcg ccccgcgcag atgcgcatcg ccagcgagtc
     1441 agagccagcg cgcgatcgag gtaggacggg agggcggcgg ctgccccgac cgggtcggcg
     1501 agctcgcgct gggcctcgtc gagacgaccc tgttccagca gcaacgccgc atgtaggacg
     1561 ctgctcagga tcgaaacagt cgtgtcgacc tgtccgggtg cgtcgaggtc gatcctggac
     1621 aacgactcga aggcctggtc gaactcgagc cgaaggtacg acgcccacgc gagcgtgatg
     1681 tgcgacctgt cggtgaagca gcggtccgcg gaaccggttc gctgggcatg cgtgagccct
     1741 tccgctgcgg tgtcggcagc gccccgggcg ttgctcttga ggagctcacc caccgcgcga
     1801 tgtccgaggc cgagggcgag caggcccgga tgatccgatg cacgtgcgct cagcagggct
     1861 tcgtcgacat ggacagcggc cgagtcgagg tcgcccgccc acatctctgc gctggcgagc
     1921 tcgagcagga gccaggcctg ccgctcgggc gacagcacca gcacgggtcg gtgggcatgt
     1981 gtcgaggact gcagacagcc gagcacccgc cgagcctcgg cgatgaccgc ggaggcgtcc
     2041 tgccaaccga gatgcgtctg ccacagctgc aggatcatcg cgtcggcgcg aacggcatcg
     2101 cgcagcaacg actcggtgac cgtttccgcc ggcatcgtgg cgcccgtctc ctgtgcgtgt
     2161 gcggcgagaa cgaccgcgcc gccgacgtcg ccgaccgcgc gacggtacag accgacgatg
     2221 ccgcgcagga agggaaagtt cgtcatcgcg tcatagccca gctcgtcctg cgcctgcgca
     2281 acgacctcca gatctcccct tgtcagcagg gccggtccgt gttcgaccag cagctctgcg
     2341 accatctgcg gctcgtgtga ggcaatggcg tggcggaggg ctgacgcgcg cttcccctgc
     2401 gcggcgtcat accttgcggc gcgtagatgg gcgtccgcca cagtggacac gtcgccgctg
     2461 tcggcggtga tccgacgacg cagcagctcg acgagcagcg ggtggtagct gtagaccatc
     2521 tcgctggtgg actggccggc gtacgccgtg acgagcagcc cgtcggcggc gaggtcggac
     2581 agcagggccc cggcgtcgga tcgtccggtc agcacgacag cgcgcaaacc tgtcagctcg
     2641 ggctcctcga acgtgctcag caacaactcc ttgacgccgt ccgacagcgc ggcgaacgcc
     2701 tctccgagca ggaagtccag caccggctgg tcggtgccac ccaacgcgga acggatcgcc
     2761 tccggttcgg agacaccggc gaccgcgtgc gcaccgagca cgagcgcagc cgcccatccg
     2821 tcggcacgcc gggccagctc gacgatcgcg gccggtgaga catgtgagga gtgctgccgg
     2881 acaagccgtt ccgactcgtc cggggtgaac cgcagctggc cggctcgcac ggtcgtggcg
     2941 gcgtccttga gctccagctc gagcaccggc acggcgagat cgcgtcggct gccgatgagc
     3001 aggttcaccg cctccggtcg cgtggtcagc acccgtgcca ggagtgccac cgtgctcggt
     3061 gggaggaggt gagcgtcgtc gacgatgagg aggtcgggcc ggtgcccgtc gtcgaaccgc
     3121 tggacgaggg catcgtgcac gcgcggtgac gcgacacctg gcttgccggc ggtcagggcc
     3181 gccgcgtcct cggcgtccac accggccgcg atcagcagca gctcttcgac gctgatcccg
     3241 tcgtgcgggc tgttccaccg agcacgacgc aaccgcgtcg atctggactg ctcgagccag
     3301 gaactgagga gaacggtctt tcccgttccg gccggcccga tcaccaacgt caatggcttg
     3361 cgagtgccac tgtcgagcag tttgaacagt cgaggacgcg ggacgagcag cgcgggagtg
     3421 cgcggcgcac gacggagacc gacgctgggg acggctgagc gccccgtggt cgtcgtcttg
     3481 cgcggcatgg ctcccctccc gaaaagccag cgcatcacgg aggtcgtgac gacgcgtgac
     3541 gatctcacga tacgtgggag cgccgctacg attcgcgttt ccggtacgcg agtcggaccg
     3601 atccgtcacg acttgtcgga gactccgcca ctgtcgcccg gggcaaatcg ggtggtttga
     3661 tccacggttc cggagatcag cggctgcaac cagcccgcgt ccctgtcggc atcgacgagc
     3721 accgccttcg ccagcaacga cagcggaatc gccaacagcg cgccgagcgc gcccagtacc
     3781 cacgcccaga acaccagcga gaccatcgtg atcgtgggcg tcagtccgac ggcgttgccg
     3841 acgatcttcg gttggatcag cgactggatg acgaagttga tcacgctgta cgccgcgatg
     3901 accgcgagcg ccaaccccgg cccgccttcg aggtaggcca gcgcggccgg cggtaccagc
     3961 ccgaccacga acccgatgtt cgggatgtag ttcgtcacga aggacaggac cccccacgcg
     4021 aatggcgccg ggatgcccag cagcgccagc gcgatcacgt cgatgacggc gacgatgaac
     4081 ccgaagcctg aggagacgac aaggtacgtg cgtgtaccgc gggcgaacga gatgagcgag
     4141 tcgaccatct gtgggtgccg gttccgggca aactccagga gcgtcgggaa ctggtaggag
     4201 tcgatcgcca tgaaaaacag cacggtgagg atgaagaaca ggctcgatga gatcgaggcg
     4261 agttcgccga ggaggtcgcc gacgtacccg accagccggg ctgggtcgat cgagcttgcg
     4321 agcgactgct gctgttcggt gtggatgccg agctggtcca gccggttcgt cacgtcctcg
     4381 atgcgctgct gtgcctcgtc ctggtagctc ggcagcaacg aggcgaacct ggcgagcgcg
     4441 aggacgaccg atcccacgat ggcgatcagg atcacgaaca cgccgagcgc tccgacaagg
     4501 aacgcagacc accgtggcca gccgcgacgg atcacgtagg tctgcgcggg atacacagcg
     4561 atcgtgagga cgagcgccaa gaagacatcg ctgacgatga acgacgctga gcgcagcccg
     4621 ccgacgacta ccaccgcagc tgccagcccc aggagcagga caaccgttct cgggagaccg
     4681 ttcgacaggc ctggcggtct cccggcgacg tcgctcatac cggcatgacg tcttcgttct
     4741 cgggcagtcg atgcacctcg acgacgtcga caccgatctc tcccaggtag ccgagcacct
     4801 ccgacagctc ggactgcttg tcgaggtggc cgtgcagcac ggtctgggcg tgctgggtgg
     4861 cttccagatg cgggaacgtc gccatcagtc cagcggacag ctgcccttct acgcggatga
     4921 tgtagtaggt catcgacggc tccttgtcgc gtccagcgca cgacccgact gaagccatga
     4981 tcgtgttgcg aagcaggcac agcctcaccc gttagtggtg aggctccagc cgggtcggat
     5041 ttctaacctg aggttgaggt gtgagtgccg tgctcgcgga acggggcgcg ccgtatggag
     5101 gaccgaaggt gagcggggat gacgcgaacg gcactcagag cgaggctcgg ctccgttcgc
     5161 ggctcgcacg tgcgatcgac aggtcgcgca gggttgcgct ctttctcgtc gacgtcgtcc
     5221 ccgggcttcg tcggctgttg gccgaagtgg cccgcaacga gatcatcgac cggtccctcg
     5281 tcgtcgcggc acaggcgatg ttctccgtca cgccgctgct catcgtggtc gcagcgttca
     5341 gcccacgcac gttcggtgac agtctctcgg agagcatggc caacgtgatg ggcgtgcaac
     5401 aggaggacac gaccgccctc cagcgagcgg cgacggtcga ccaggtgcgc gcgcagaccg
     5461 ggatcgtcgg cgtggccatc gtcttggtgt cggcactgag ttttgcgcgc gccctccaac
     5521 ggatgtacga acgcgtctgg cagcagcagc accgcggtgg tgtggtgggg aaccgccgct
     5581 gcttcttctg gctcgtgagc tggatcgcgt atctcgagat gatcgcggtg ctctcccacg
     5641 tcttgccgcg aggcgacctg tcgacgctcc gactggtcgt gcagttcgtc gtgagcacgg
     5701 cggtctggtg gtggacgatg cgcatgctct tgttgggccg cgtcggctgg ccgaacctcc
     5761 tgctgggagc cctgctcacg tccatcggtg tcttgctgct cacccggttc tcacgactgc
     5821 tgatgccggc gtacgtcggg agcgcggttg accagttcgg gacgctcggt gtcatcttcg
     5881 cgatttcgac gtggctgctc gtgttcggag ggatcttggt ggtggcggcg atcatcggcc
     5941 gggtgtacac cgaggagctc acgatcatgc agcgattcat ccgcatgttt gcaaagagaa
     6001 cggcaccaga ttcccaaccc gctgggggtg cgtgatggac gacgacacga cggcaaacgg
     6061 accggacgag gatcgccccc ctggttctga cgaggagttg aaagcggagg ttgccgcgct
     6121 gcgcgccgag ctcgcaacgc ggccggccgg cggacgctcc agcaagggtt ggtggcggcc
     6181 gctggtctgc ggtttgctgc tcgccctcgt cgtcgtcctc tcaccgctga gcgtcctggc
     6241 ggtgtggacg catgccgaga tcgccgacac cgaccgctac gtcgatacgg tggcgccgct
     6301 ggcgtccaac ccggatgtgc aggatgcggt cgccaaccgc atctcgacga cgctgctcac
     6361 gtacatcgac gtgccgaagc tgacgtcgga aaccgtcgag gcgctgtcgg cacgtggtct
     6421 ctcggaccga gccgcggcgg cgctcacggc gctcagtgtc ccgctgacca acgcggtgac
     6481 cgacttcgtg cacaccaagg tgctcgaact cgtacagagc gatgcgttcg cacaagcgtg
     6541 ggaggaggcc aaccgccagg cccatgagca gatggtcgct gtgctcaccg gcgagacgaa
     6601 cggggcggtg agcgtctccg gcaacgaggt cagcgtgaag cttgccacct tcatcgacac
     6661 cgtgaagcag gcgctggtgg atcaaggatt ccagttggcg agcaggatcc cgaccgtcga
     6721 tgcgacgttt gtcgtcttca gttccgacaa tcttggcaag gcgcagcagg gcttccacct
     6781 gctcgacacc gcggcgatcg tgctgccgtt gctcacactc gtcctgatcg tcgtggccgt
     6841 cttcgtcgcg cgggtccggc gtaccgccat catcgcggca ggtctgtgtg tggccggggg
     6901 gatgctcctg ctgggtcttg cgct
//
LOCUS       DATBFZ010000021        29865 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_1325487, whole genome shotgun sequence.
ACCESSION   DATBFZ010000021 DATBFZ010000000
VERSION     DATBFZ010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 29865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29865
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1325487"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..824
                     /locus_tag="VH419_00555"
     CDS             <1..824
                     /locus_tag="VH419_00555"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEY7042137.1"
                     /translation="GPLTIGLESSGQLAQRVGVSGPTVSRFVSRLGFDNYGAFQRALR
                     EDLQARVKSPIEVYRTHVDPKVDRDATSPLEAAGDVLAEAVVATLRQVGPTDFQDATT
                     YLTDSRRQVLAAGGWFSHLLAGYLTALLRQFRPRVHLVQPVASERAAALADLETRDIV
                     AVFDFRRYERDTFEFAKEAKHSGARIVLFTDPWLSPVADLADAVLPAHVVGPSPFESL
                     TPTLALVETLVTAVAQGMGELGDRRFSHFGGIADHWVRPWPTHDNSSDLIDQDLP"
     gene            866..2191
                     /locus_tag="VH419_00560"
     CDS             866..2191
                     /locus_tag="VH419_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020391650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEY7042138.1"
                     /translation="MHVSRVHARSTLALVATGALAAATALAPTSAALAKKEVSAVRHI
                     VYQQWDSASDFAAGTASGTTVVGDTLRIGTPSGQFSYTDPFGAGTTATYDVGSWTSPV
                     VTTGFPYTELVSSWNAKTPPGTWVQMSVRGLADNGSTSKDYILGRWAEGTESIHRTSV
                     PVQGDDLASVAIDTLVAKKGRSLSTWQLTVSLYRLAGSSSTPTVSLVGAMASGLPDVK
                     KVNASPLGGAEGIELAVPPYSQEIHRGHYPEFNNGGEAWCSPTSTAMVLGYWQQQFPG
                     QGYGPAPADYADLIPPDQWVDYAAANTYDWNYDGTGNWPFNTAYAGRYGLEGFVTRLR
                     SLTEAEQFIKAGIPLVVSVSFKQNELTGAGYGTNGHLMVIRGFTPSGDVIANDPASHL
                     DPNDASVRVVYNRAEFENVWIPKSGGIAYVIHPESVSLPPNVPGAEPNW"
     gene            complement(2295..4031)
                     /locus_tag="VH419_00565"
     CDS             complement(2295..4031)
                     /locus_tag="VH419_00565"
                     /inference="COORDINATES: protein motif:HMM:NF044955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEY7042139.1"
                     /translation="MSRKHVRGLGSGWWTATHAGVFVAAFLGTMALGPPVAVAAAATT
                     IGTYDATVRVNEDGTLHVSESVTYDFGGAATDAVERVITTREQYDADDDRVYDISDVS
                     VDAVQTDVKTTVTSQDTTDVIDVEFTEPQSGAVTVTYDYDVDGTVAETADGLEVRWPV
                     VQGFDAPIEHATVEWDAPDAIWMSCLAGSPGSSRPCTTSQLVDVSAPTMTQDGLDAGD
                     EMVGILGLDAASGVSASTDLRPRWSLARAFTASGPQLVVAMAVVLLGLLGAVLLWWTR
                     GRDRGHRGDVDVCPLLDRGDGTLVFAPPSAVRPGQMGTLVDEHADVVDVAATVIDLAV
                     RNYLFVEEQVGDPSGRADWLLRRRNDAGDELLPYEREVFSALFGQGDDVLVSDLDSAM
                     RSRLPGVQALLYDDMVREGWFGERPDAVRSRWTTAGWVLVTAGLVLTGVLALVSTFGL
                     VGLAVVLAGVSLAAAGQVAPARTSRGSKVLRELREYRAFLADAPTDEIPSEQREELVS
                     RCYPYAVVFGLGERWAEALATMDVDDSPDEPLYWYGAPEDWHLSDAATSLASFSAALT
                     SALAGRRLLTFD"
     gene            4433..5290
                     /locus_tag="VH419_00570"
     CDS             4433..5290
                     /locus_tag="VH419_00570"
                     /inference="COORDINATES: protein motif:HMM:NF016143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HEY7042140.1"
                     /translation="MAAGFFVLVLIGAALQDGGTRARVDASAVDHRPARAENPGSAPV
                     DPSDANAVVRRNTLYTEGGLANGNCPAKDLGDAGRQEQTAFYRALMECLNDEWRPLVE
                     NAGYSYEEPGLVVFDSPVDTPCGNASPESGRTLAFYCPGDSVMYADVPQMRRFFGDDD
                     VAYAIVIGHEFGHHVQQETGILSAYDDLAHADFESRLELNRRVELQASCMGGLFLGAI
                     AESFPMSDQRLSRLEQVAGSFGDEPGGGDDKRDHGSGASNRAWIFRGFDANDAETCNT
                     FVVPAGQVE"
     gene            5291..6106
                     /locus_tag="VH419_00575"
     CDS             5291..6106
                     /locus_tag="VH419_00575"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY7042141.1"
                     /translation="MHRSGVGGRGFGQWIGAVIDVPPAVVDAVDAFWTGVLGWTVGPA
                     WPGHPEFHHLEPPAGDSHVLVQAIDGPARIHLDLYVADIDAEAARLVALGATLGERHA
                     HWRVLSSPTGLPFCVVTSDRARERPSAPTWPDGHRSRLAQVCIDVPHGGLDEETAFWQ
                     AVTGWTFRESDVPEFAGHLEPGPGGSMQLLVQELGADDPAGGSRVHLDLGTDDIEAEV
                     CRLVELGADRVRDDRGWVVLRDPAGMLFCVTVQSPSEATLPPEKRGLDEPGTR"
     gene            complement(6210..7823)
                     /locus_tag="VH419_00580"
     CDS             complement(6210..7823)
                     /locus_tag="VH419_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020390657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEY7042142.1"
                     /translation="MTSQVRTTPEDRTRPARSSRLVIGAVLGMVSAGAGLAVSELLSG
                     WLHQRASPVVALAESVIRLTPGAVIEHVISLVGHNDKPLLVSLTLLGLALASSIVGVL
                     ALRNLILAELIFLIMGVVLATAVHARLTSSPVRYVPAIAGVAVAMFALAQLAPRAVRV
                     VSPGPIDTTSPADGASTALASREQDLSRRRFLATAGVVAAGALVVGATGRWLARSRAA
                     VEAARHRLRLPITARPAPSGADLGVDGVAPWVTSQQDFYRIDTALAVPQVLPAEWRLR
                     IHGMVERELTMTYQDLLDRGLQQAWITLCCVSNQVGGGLISNASWSGVPIADVLADVG
                     LHPDADAVLSTSADGWTAGTPLSVLTDGRDAMFAVAMNGQPLTLEHGFPVRMVVPGLY
                     GYVSGTKWVVDLEVSRFDRFSAFWTQRGWSERGPIKTESRIDVPDDGAHVSAGQVVVA
                     GVAWAQHTGIDRVEVRIGDGDWQQAKLAIEPTIDSWRQWSYLWSAEPGQHDISVRATD
                     ASGYTQTGDRADVVPNGATGWHTISVDVS"
     gene            7944..8441
                     /gene="ispF"
                     /locus_tag="VH419_00585"
     CDS             7944..8441
                     /gene="ispF"
                     /locus_tag="VH419_00585"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020578975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEY7042143.1"
                     /translation="MTSEPAPASIRVGVGVDVHPLVAGRELWVAGLLWPDERVGLSGH
                     SDGDVVAHAACDALFSAAGLGDLGSQFGTSEPSWEGASGVALLAETARRLRLAGAGIG
                     NVAVQLIGDLPKIGPRRAEAERVLSDAVGASVTLTATTSDGLGLTGRREGLAAVATAL
                     VYVGS"
     gene            complement(8426..9139)
                     /gene="ispD"
                     /locus_tag="VH419_00590"
     CDS             complement(8426..9139)
                     /gene="ispD"
                     /locus_tag="VH419_00590"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEY7042144.1"
                     /translation="MLTHVSQVAAIVPAAGSGSRLAAAPTDSGLPKPLRRLGRDTLLR
                     HAVRSVERHADQIVVPAPAEWIDEIRASLADVPLVVDVVEGGPTRQRSVELALAAVER
                     ATPYVLVHDAARPLTPSAVTERVVAALRAGAQAVVPAVDVVDTLRQVTTDNTSRTMDR
                     SSMRAVQTPQGFATEVLRQAHIRAGHDQATDDASLAESLGVDVVIVEGDVLAFKVTTA
                     LDLRLAEVVVSRDEAQEPT"
     gene            complement(9387..9872)
                     /locus_tag="VH419_00595"
     CDS             complement(9387..9872)
                     /locus_tag="VH419_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014136563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HEY7042145.1"
                     /translation="MTFTVGETVVYPNHGAAVIEDIETRNIKGEDKLYLVLRIVAQND
                     LVVRVPACNLDLVGVRDVVDSDGLERVFDVLRAEHTEEPTNWSRRYKANLEKLHSGDV
                     LKVAEVVRDLWRRERERGLSAGEKRMLAKARQILVSELALAEHTNEDKAETILDEVLA
                     S"
     gene            10167..10748
                     /locus_tag="VH419_00600"
     CDS             10167..10748
                     /locus_tag="VH419_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042146.1"
                     /translation="MSARPSARSRLPRRLAIAALVATLPVLTACAANFSAQTQQQYQP
                     AVGTDDRDGDVYVINAVIVADQAGNGTVVTTLISQQSDDYLQGFSAEDSKGGALKTNK
                     LPAITGSQDPNAPSNGIALPAQQSVKIPDDGALQVSGDTITPGTFATLTLTFVEAAPA
                     TIDVPVVPRSGPYIGTQIRPIETPSTSESTPAS"
     gene            complement(10778..11467)
                     /locus_tag="VH419_00605"
     CDS             complement(10778..11467)
                     /locus_tag="VH419_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020578980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY7042147.1"
                     /translation="MTRVLVVEDEESYSDALAYMLRKEGFDVALAGNGPEALEEFERG
                     GADIVLLDLMLPGLPGTEVCRRIRQVSSVPVIMVSAKDAEVDKVVGLELGADDYVTKP
                     YSPRELVARIRAVLRRGSEQDLTPVALEAGTVRMDVDRHVVTVSGEEVRLPLKEFELL
                     EMFLRNAGRVLTRGQLIDRVWGADYVGDTKTLDVHVKRLRAKIEPDPAHPRFLVTVRG
                     LGYKYDAPAHV"
     gene            complement(11464..12708)
                     /locus_tag="VH419_00610"
     CDS             complement(11464..12708)
                     /locus_tag="VH419_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY7042148.1"
                     /translation="MDSSTAALLGGGIGAMLGAFAVLAFLRSEHIQSVSPSAPPAEPL
                     VPPGVAAVLSVLRSSALVVGPDDQVLKASAPAHALGLVRDSRVAVAELLELVRQVRRD
                     GQIREDELVLRRGRTVSRHVAARVAPLGSRLVLVLVEDRTRERRVEAIRRDFVANVSH
                     ELKTPVGALNLLAEAVGEAAEDPQAVERFAVRMRTESQRLTRLVQQIIELSRLQGDDP
                     LDELDPVSVDALAERAIDRVGVDAHDKRVELSFAGEHGFQVLGNGEQLLVALGNLVEN
                     AVAYSPPDTKVTVSARANEDDDDMVDIVVTDQGVGIPADDLDRIFERFYRVDPARARS
                     TGGTGLGLSIVKHVAATHGGDVRVWSVEGAGSSFTLTLPSRNVDAQDPIGLGVGLSDG
                     LDTDGRDGVLHSVSRSHAEEAS"
     gene            12843..13496
                     /gene="phoU"
                     /locus_tag="VH419_00615"
     CDS             12843..13496
                     /gene="phoU"
                     /locus_tag="VH419_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEY7042149.1"
                     /translation="MRVQYQQQLDHVLSELVVMTGGVREALSQATKALLQADASAAES
                     VISGDLAIDDAREQIENESFEILARQAPVAGDLRMLVASLRMVADLERMGDLSVHVAK
                     VARLRFPTKAVPDVLEPVISEMAKTADEMIGAAADIVANRDVRAAQELEEADDEMDRL
                     RRSMFRTLLSDEWDAGVEPAIDMALLGRYYERIGDHAVSMARRIVFLVTGENPTALL"
     gene            13637..14392
                     /locus_tag="VH419_00620"
     CDS             13637..14392
                     /locus_tag="VH419_00620"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007030857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglyceromutase"
                     /protein_id="HEY7042150.1"
                     /translation="MSDVPYRLLLVRHGESEWNAKNLFTGWVDVALSEQGRGEAVRAA
                     ELIAERDIVPDVLHTSVLRRAIATAQICLDEIDRHWIPVRRSWRLNERHYGALQGKDK
                     KATLEEYGEEQFMLWRRSYDTPPPEIADDDEFSQAGDPKYAALPPELRPRTECLKDVV
                     ERMLPFWYDAIVPDLLTGQTVMVAAHGNSLRALVKHLDDMSDEAVVGLNIPTGIPLLY
                     DLDHELRPVTRGGTYLDPAAAEEAIKAVANQGR"
     gene            complement(14487..14645)
                     /locus_tag="VH419_00625"
     CDS             complement(14487..14645)
                     /locus_tag="VH419_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042151.1"
                     /translation="MLTATDVAREVSLSRRAEARESELISAARLARRQRRLERRIERL
                     NGYLAAHR"
     gene            complement(14844..15386)
                     /locus_tag="VH419_00630"
     CDS             complement(14844..15386)
                     /locus_tag="VH419_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020392203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HEY7042152.1"
                     /translation="MTSSSDDIAVARATAIDTIRRALADAEIDVREPRDGVFVFDLPG
                     ERKLATPCQLAVGDHAVAVHAFVCRNPDENHEGVYRWLLSRNLRLYGVAFGVDHNGDI
                     YLDGRLALSAVSPGELDRLLGSVLSYADESFNTILELGFASSIRKEWEWRLSRGESTR
                     NLEAFRGWLERDDAPAPPAP"
     gene            complement(15383..16693)
                     /gene="mshA"
                     /locus_tag="VH419_00635"
     CDS             complement(15383..16693)
                     /gene="mshA"
                     /locus_tag="VH419_00635"
                     /EC_number="2.4.1.250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012918668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010125 - mycothiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-inositol-3-phosphate glycosyltransferase"
                     /protein_id="HEY7042153.1"
                     /translation="MTATLARVVPFDRALTPRRIAMLSVHTSPLDQPGTGDAGGMNVY
                     EVELSRQLAARGIEVEIFTRATSGRLPPTVELTDGVTVRHVVAGPFEGLTKGELPGQL
                     CTFARDVLRTEAAYAPGYYDIIHSHYWLSGQVGALARDRWGIPLVHSMHTTAKVKNHL
                     LADGDVPEPMARVIGEEQVVETADLIIANTADERRQLLDLYAAEPERLRVITPGVDLR
                     VFRPGSQTDARRRLGVREDALVVMFVGRIQPLKAPDVVVKAAARLVDRRPDLRDRLVV
                     PIIGGPSGSGLETPDGLVDLAASLGVGDIVRFVAPVEQARLVDYYRAASLVAVPSYNE
                     SFGLVAIEAQACGTPVVAANVGGLPTAVDDGVSGILVDGHEPAQYAAVFEWLLTDDLA
                     RRRLQAHCAAHARRFGWGQTAQLTLEAYRDAALSMRADALALAE"
     gene            16846..17616
                     /locus_tag="VH419_00640"
     CDS             16846..17616
                     /locus_tag="VH419_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007076578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY7042154.1"
                     /translation="MSDPAQAGPDGPRLAVVTGASSGIGAAVARALARVGFGVFCAAR
                     RLDRIESLAEEIGGEAVACDVTDEAQVADLAAAVGSRLDVLVNNAGGAFGTEPVAEGR
                     VEDWRAMYDVNVIGTLNVTKALLPALIASGDGVLVNMGSTAGRVAYEGGGGYTAAKHG
                     VAVMTETLRLELVGQPVRISEIAPGMVKTEEFGLVRFRGDEEARDAVYEGVPDPLSAE
                     DIADAVAWVATRPSHVNIDLMVMRPRAQAAQHKVYRET"
     gene            complement(17622..18455)
                     /locus_tag="VH419_00645"
     CDS             complement(17622..18455)
                     /locus_tag="VH419_00645"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HEY7042155.1"
                     /translation="MGAPRGKRACRLFAKMGDMPTSLTLDRHLEAIRRSGPALADRAE
                     LAGLDAPVPTCPDWTIAHLVAHLAMAHRWAAAHVRRDVDREPLDDDVIRTTQPDLVGY
                     YREEYDGLVRALEAAPADLDAWVFLNDAPAPREFWARRQAHETTIHAVDALAGRLGAI
                     PTAAECAIDLELAIDGIDELLRGFITRRPSRLYAGKEFTILVRPTDHDRAWLVSVSAE
                     PPVTSELHPGTEGAEVAAVFFGTAAQLYVGLWNRGDEITVEGDQGMLDRWRSAHHIRW
                     G"
     gene            complement(18588..19820)
                     /locus_tag="VH419_00650"
     CDS             complement(18588..19820)
                     /locus_tag="VH419_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020386358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY7042156.1"
                     /translation="MPVIDVRDVVRNVEEAHRSHWATVLAATVRLTRDIDLAEDCAQD
                     AFVQALRSWPDGIPDNPAGWLTTVARRKALDRLRREDSLRRKLPLLLVEETSESPDAD
                     LTAPADPLRLVFTCCHPALGRDAQLSLTLRLMCGLTTAEIGKVLLTKEATIAARVTRA
                     KQKIAAAGVPFRLPTDDQLPERLDTVLTVVHLAYTAGHTATGDALTRPELTDRALRLA
                     RLLVAQLPDEPEAQGLLALLLFAQARGAARLGPAGDLVLLADQDRSRWDTTLVHEGLA
                     LTTQALDGGGRFALQAAIAGLHMTAPSWETTDWRQVVRMYDALLLTWPSPVVALNRLA
                     ATSLVPGADLPAVLAELDVLALDPTLERYAYVAATRADVLSRLGRQPEARAAYDIAIE
                     LSDNETERRFLRARRHSL"
     gene            complement(19865..20239)
                     /locus_tag="VH419_00655"
     CDS             complement(19865..20239)
                     /locus_tag="VH419_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012922529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEY7042157.1"
                     /translation="MAQYMFLLFDDEKWYDELTPESWETAMKLHGEFATAVEAAGARI
                     LEGRALERSSTATTITKDWSDPSAKPVATDGPFIETKEALGGFYLVEARDLDQALELA
                     NICPSGHVEVRPVMDTSGDAPS"
     gene            20384..22303
                     /gene="typA"
                     /locus_tag="VH419_00660"
     CDS             20384..22303
                     /gene="typA"
                     /locus_tag="VH419_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEY7042158.1"
                     /translation="MPGTDLTPTSRADLRNVAIVAHVDHGKTTLVDAMLWQSGAFGEH
                     QHVDERAMDSGDLEREKGITILAKNTAVRYTPASKSDAAAASQPQRAVTINIIDTPGH
                     ADFGGEVERGLSMVDGIVLLVDASEGPLPQTRFVLRKALSAKLPVVLVVNKVDRPDAR
                     ISEVVEETYELFLDLLDDSHDQDALDFPVVYASAKAGRASLEQPADGDLPDSPDLEPL
                     FRTIIETVPAPTYVDGAPLQAHVTNLDASPFLGRLALCRVHEGTLRKGQTVTWIKRDG
                     SQQNVKITELLVTEALDRVPGDSAEPGDIIAVAGIPDINIGETLADVDEPVALPLITV
                     DEPAISMTIGTNTSPLAGRVKGSKVTARLVRDRLDRELVGNVSIRVLATERPDAWEVQ
                     GRGELALAILVEQMRREGFELTVGKPQVVAREIDGKVHEPVERLTIDCPEEYLGTVTQ
                     LLAVRKGRMEQMVNHGTGWVRMEFLVPARGLIGFRTDFMTDTRGTGIAHHVFEGYEPW
                     FGELRTRPSGSLVADRTGTATSYAMFNLQERGSLFVEPGTEVYEGMLVGENSRSDDMD
                     VNITKERKLTNVRSSTAEELERLVPPRRLSLEQSLEFCREDECVEVTPEAVRLRKVVL
                     DQHERAKSAARSRRT"
     gene            complement(22412..22906)
                     /locus_tag="VH419_00665"
     CDS             complement(22412..22906)
                     /locus_tag="VH419_00665"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HEY7042159.1"
                     /translation="MEITLVEGDITEQQVDAVVNAANRGMRGGGGVDGAIHRAGGPAV
                     LQDCIERFPNGLATGHAGWTTAGRLSARWVIHVVGPNHAAGETDRSLLTSCYANALAV
                     ADELGARTVAFPLVSAGIYGWPLQDAANAAVETLRSTPTSVEEARMVAFGSTAFAVLE
                     LALT"
     gene            complement(23272..24051)
                     /locus_tag="VH419_00670"
     CDS             complement(23272..24051)
                     /locus_tag="VH419_00670"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY7042160.1"
                     /translation="MPLIAGPDGPIECLVNGSGLPVTVFAHGLASSIDETRPFGSGVS
                     GTRVFFHFRGHGASGGSEAPWTYAAAAAELDAVRTAYDAHQALGVSLGAGALLRSAVD
                     TPDAFRRLVVVLPASVDQPRTDPAIDRMQARAALIEHRDLRSLAESLVAEQPVGVRDR
                     SDVRLWARRQARRLSATTVARALRELPPQYPLKSRAELAVVRCPVLVIGQEGDGAHPA
                     SVARDLAAALPDADLEIYDDGGVLWSHRAAVRRRISAFLNA"
     gene            complement(24066..24371)
                     /locus_tag="VH419_00675"
     CDS             complement(24066..24371)
                     /locus_tag="VH419_00675"
                     /inference="COORDINATES: protein motif:HMM:NF022184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2516 family protein"
                     /protein_id="HEY7042161.1"
                     /translation="MSSTAAVDAVLNLETLILFVIWILLFAVKTFALIDALFHKDNSY
                     VAADKQNKAFWLLLLVIFLVLHYLISDPRHVLNLIGTTAALVYLADVRPTLRAMKRY"
     gene            complement(24473..25207)
                     /locus_tag="VH419_00680"
     CDS             complement(24473..25207)
                     /locus_tag="VH419_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042162.1"
                     /translation="MAPAASFDKTRKDIEKAIADSGALNAVVGAGDLAVKKLRDVGGE
                     ITTRAGKIDPKALRGQAQAKVAATPEQIKALQKQAQAKVSAAPDQIKTLPTKAQSALG
                     DAVAKGFTAYGELAGRGKELVDRVRGQQSTQDLDDQASTTVSQAKGTATTAKKAAAET
                     ASTAQEAAKKTASTAKKSATRTKTSVKSTTTSAKKTASAAKKAADDAATKVGDDAPKI
                     GGDSAPMSGGDTAPRSGEDTAPTSGA"
     gene            complement(25257..25688)
                     /locus_tag="VH419_00685"
     CDS             complement(25257..25688)
                     /locus_tag="VH419_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY7042163.1"
                     /translation="MATLKVGRPLTEGAPKAAARVTATARASVAGTAQNLGDYLRDQR
                     RGAQLTLRQLADQAGVSNPYLSQIERGLRRPSADVLQQLAKALRISAETLYIRAGILD
                     PDERGAATVQTAVLGDPDLNERQKQVLLEIYASFRQGNATT"
     gene            complement(25864..26826)
                     /locus_tag="VH419_00690"
     CDS             complement(25864..26826)
                     /locus_tag="VH419_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020392224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEY7042164.1"
                     /translation="MADTEILVDIVRSGFVEGHHRGSVVALDTEGSIDWSIGDVTSPI
                     YPRSCNKPVQALAMLRLGLDLDGELLALAAASHSGESFHIDGVRRILSSAGLDESALQ
                     TPPDFPLDDAAKTAYIRAGLEKSAIAMNCSGKHAAMLATCVERGWDIATYRLPQHPLQ
                     EAICVTFAELTGEPVVSIGVDGCGAPLFSATLLGLARAFRSIRVAGAGSPERRVAEAF
                     AAYPEFASGSTRDEAKLLRAVPGAMAKAGAECCYALALPDGRAVALKVEDGSPRARPV
                     VMAAALQRMGVMEDDAVDVEALRATGVVPLLGGGEPVGELRPAF"
     gene            26893..28200
                     /gene="cysC"
                     /locus_tag="VH419_00695"
     CDS             26893..28200
                     /gene="cysC"
                     /locus_tag="VH419_00695"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF003013.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HEY7042165.1"
                     /translation="MSTPVEGKVGVPLWTPTPRELDDLDLLISGCFAPPLPGFVAPTD
                     DSAPITLQVSGTVADMVIAVGQVDLVDPEGAPLARMTVTGTWPTDEPDGLGLVGPVER
                     LAHPEFGPFRRLHVPPATLHETVPADTLLAVPVHRVLSGRDIDEIARAAKDQDRIAVL
                     LACVGDGMPQDISGPALVRATLAAAPLIGADTAVCAVAAADHGWDAAPGVRSPNDWLL
                     DSIAAAYATAAYRVERQPTGALPDAVAAIATRDRPPRDRQGVVIFFTGLSGSGKSTLA
                     RALVDHVLEQGDRTITSLDGDVVRHHLSKGLGFSRADRETNILRIGFVAAEIARHGGV
                     AICSPIAPFESTRSEVRRMVERAGGGFVLVHVSTPLEECERRDRKGLYAKARAGEIPD
                     FTGISSPYEEPVDAIKIDTTNRDIADCLAELLDVVTTEGWLHP"
     gene            28219..29316
                     /locus_tag="VH419_00700"
     CDS             28219..29316
                     /locus_tag="VH419_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEY7042166.1"
                     /translation="MLDASQLLADSALSISSIEVKTAVAAFLVGVVVGITGMGGGALM
                     TPALIFLGVGDASSVVTADLTAAAVYKTGGAVVHARAGSPNLRLAGWLIAGSVPCAFL
                     GPHLVHWFAPEADIDTFLKKAIGFALLLAASTYALRLYLELRRRTSGRPTPSGEPVIR
                     PIPTMLIGGFGGLLVGITSVGSGSVIMVALLMLYPMLSALKLVGTDLVQAVPLVLSAA
                     ISNIMVNGLDWSIVIPLTIGSVPGCILGAKVAPMMRQTYIRRGIIVVLTMSGLALLDK
                     AGWAPLGSGEDDTHPVFIALVGVALVFLVPLVWGLLRMERGLPMFGTPTVQQIEDHQA
                     DVPDRPTGEAAPNAVEESDRTLEEPARKSSD"
     gene            29480..>29865
                     /locus_tag="VH419_00705"
     CDS             29480..>29865
                     /locus_tag="VH419_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008360685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HEY7042167.1"
                     /translation="MTVHADYRLSQLEELEAESIHIFREVAAEFEKPVLMFSGGKDSI
                     VMMRLAEKAFFPAKIPFPVLQVDTGFDFPEVLQTRDNWVDRLGVRLVVASVEQAIKDG
                     IVVDDGKTSRNRLQIGTLLNAVEENG"
BASE COUNT         4685 a        10343 c        10120 g         4717 t
ORIGIN      
        1 ccggtccgct cacgatcggg ctcgagtcgt cagggcagct tgctcaacgt gtcggcgtga
       61 gtggtcccac cgtcagccga ttcgtgagtc gcctgggctt cgacaactac ggcgccttcc
      121 aacgggcgct gcgtgaagac ctccaggccc gggtcaagtc gccgatcgag gtgtaccgga
      181 cgcacgtcga cccaaaggtc gacagggacg ccacgagtcc gctcgaggcg gctggggacg
      241 tgctcgccga agcggtcgtg gcgaccctcc ggcaggtcgg tccgacagac ttccaggacg
      301 caacgacata cctgacggac agccgtcgcc aggtgttggc ggcgggtgga tggttcagcc
      361 atctgctggc tgggtatctc accgctctgc ttcggcagtt ccggccgcgc gtgcacctcg
      421 tccagccggt ggcgagcgag cgcgcggccg cgctggccga cctcgagact cgcgacatcg
      481 tggcggtgtt cgacttccgc cgctacgaac gcgacacgtt cgagttcgcc aaggaggcca
      541 agcattcggg ggccaggatc gtgctgttca ccgacccgtg gctgtcaccc gtcgccgacc
      601 tggccgacgc cgtcctcccc gcccacgtcg tgggtccttc accgttcgag agcctgacac
      661 ccacgctcgc cctggtcgag acgctcgtga ccgctgtggc tcagggcatg ggtgagctcg
      721 gcgaccgacg gttcagccac ttcggcggca tcgccgacca ctgggtgcgg ccatggccca
      781 cgcatgacaa cagttccgac ctgatcgacc aggacctccc atgaggttcc ggggttccac
      841 cccactccga ccgcgaggtg accacatgca cgtcagccga gtccacgcac gttcgaccct
      901 cgcgctcgtc gcgaccgggg cgctggcggc cgccacggcc ctcgcgccga ccagcgccgc
      961 cctcgcgaag aaggaagtgt ctgccgtgcg ccacatcgtg taccagcagt gggactccgc
     1021 atccgacttc gccgccggga cggcttccgg gacgacggtg gtcggcgaca ccctgcgcat
     1081 cggtacgccg tccggccagt tctcgtacac cgacccgttc ggcgccggga cgactgcgac
     1141 gtacgacgtc gggagctgga cgtcgccggt ggtgacgacc ggattcccgt acaccgagct
     1201 cgtctcgtcg tggaacgcca agacgccgcc ggggacctgg gtgcagatgt ccgtccgtgg
     1261 tctcgccgac aacggctcca cgtcgaagga ctacatcctc gggcggtggg ccgaaggcac
     1321 cgagtccatt caccgcacct cggtccccgt ccaaggtgac gacctcgcct ccgtcgccat
     1381 cgacacgctg gtcgccaaga agggacgcag cctttcgacc tggcagctga ccgtgtcgct
     1441 gtaccgattg gcaggttctt cgtcgacccc aaccgtcagc ctcgtcggtg ccatggcgtc
     1501 gggacttccg gacgtcaaga aggtcaacgc cagcccgctg ggtggcgcgg aaggcatcga
     1561 gctggcggtg ccgccgtact cgcaggagat ccaccgcggt cactacccgg agttcaacaa
     1621 cggcggtgag gcgtggtgca gcccgacgtc gacggcgatg gtgctcggtt actggcagca
     1681 gcagttcccc ggacaggggt acggccccgc gccggcggac tacgcggacc tcatcccgcc
     1741 ggaccagtgg gtcgactacg ctgccgcgaa cacgtacgac tggaactacg acggcaccgg
     1801 caactggccg ttcaacaccg cgtacgccgg tcggtacgga ctcgagggct tcgtgacgcg
     1861 cctccgctcg ctgacggagg ccgagcagtt catcaaggcc ggcatcccgc tggtcgtgtc
     1921 ggtgtcgttc aagcagaacg agctgacggg cgccggctac gggaccaacg ggcacctcat
     1981 ggtcatccgg ggcttcaccc cgagcggtga cgtgatcgcc aacgacccag cctcccacct
     2041 cgatccgaac gacgcctcgg tgcgcgtcgt ctacaaccgg gccgagttcg agaacgtctg
     2101 gatcccgaag tccggcggca tcgcctacgt catccacccc gagagtgtct cgctgccacc
     2161 caacgtcccc ggcgccgaac ccaactggtg acaaaccttc gtcgaaccgt gagttgtggt
     2221 tcgtccaacc gtgagttgtg gttcgtccaa ccgtgagttg tggtgcatcg aaccgccgcg
     2281 ggcggacgac aaggtcagtc gaaggtcagc aggcggcgcc cggcgagcgc cgacgtgagg
     2341 gcggccgaga agcttgccag cgaggtggcg gcgtccgaca gatgccagtc ctccggagcg
     2401 ccgtaccagt agagcggctc gtccggcgag tcgtcgacgt ccatcgtcgc cagcgcctcg
     2461 gcccagcgct cgccgagtcc gaacacgacg gcgtacgggt aacagcggga gaccagctcc
     2521 tcgcgttgct ccgacgggat ctcgtcggtc ggggcatcgg cgaggaaggc gcggtactca
     2581 cgcagctcac gcaggacctt gctgccgcgt gaggtccggg cgggtgcgac ctgtccggct
     2641 gccgccaacg acacacccgc gaggacgacg gccaagccga ccagtccgaa cgtgctgacc
     2701 agggccagta cgccggtgag gaccaggcct gccgtgacga gtacccagcc ggccgtcgtc
     2761 cagcgagacc gtacggcgtc cggtcgttct ccgaaccagc cctctcggac catgtcgtcg
     2821 tacaacaacg cctgtacgcc gggcaaccgc gaccgcatcg ccgagtccag gtcactcacc
     2881 aacacgtcgt ctccctgacc gaagagcgcg ctgaacacct cccgttcgta gggaagcagc
     2941 tcgtcgccgg cgtcgttgcg gcgacggagc agccagtcgg ctcggccgga cgggtcgcct
     3001 acctgctctt cgacgaacag gtagttccgg accgcaaggt cgatcaccgt tgcggcgacg
     3061 tccacgacgt cagcatgctc gtcgaccagc gttcccatct gtccgggacg gacggcgctc
     3121 ggcggcgcga acaccagcgt cccgtcgccg cggtccagca acgggcagac gtccacgtcg
     3181 ccacggtgac cgcggtctcg gcctcgggtc caccacagca gcaccgcgcc aagcagtccc
     3241 agcagcacga ctgccatggc gacgacgagc tgtggtccgg acgccgtgaa tgcgcgcgcg
     3301 aggctccatc tcggccgcag atccgtgctc gccgagaccc ccgaggcggc gtccaggccg
     3361 aggatcccga ccatctcgtc accggcgtcg aggccgtcct gcgtcatcgt gggcgccgac
     3421 acgtccacga gctgcgacgt cgtacacggt cggctcgagc cgggtgaacc tgccaggcac
     3481 gacatccaga tcgcgtccgg tgcgtcccat tccacggtcg cgtgctcgat cggcgcgtcg
     3541 aagccctgca cgactggcca ccggacctcc agcccgtcgg cagtctcggc aacggtcccg
     3601 tccacgtcgt agtcgtacgt gactgtcact gcgccgctct gcggctcggt gaactcgacg
     3661 tcgatgacgt ccgtggtgtc ttggctggtc accgtggtct tgacgtcggt ctggaccgcg
     3721 tccacagaga cgtcgctgat gtcgtacacg cggtcgtcat cggcgtcgta ctgctcacgc
     3781 gtcgtgatga cccgttcgac ggcatccgtg gccgctccgc cgaagtcgta cgtcaccgac
     3841 tcggagacgt gcaacgtccc gtcctcgttc acccgcacgg tcgcgtcgta cgtcccgatc
     3901 gtggtcgcgg cagccgcgac ggccaccgga ggacccagcg ccatggtccc caggaaagcc
     3961 gccacgaaga ccccggcatg cgtcgcggtc caccagccgg acccgagacc gcgcacatgc
     4021 ttgcgtgaca tggcgtcagg gtaatgtgcg ctggagttgc ctcggcggtg gcgaggcgaa
     4081 gggggttcgg gtgtcacagg gaccagacga gccgacccgt tcaggcgagt cgaccgcttc
     4141 gggccgcccg gatgccgacc gtggatggtc ggctcggccg ctgccgccac gtccggtcct
     4201 ccctccgcca cagagcgctt ccaggttcgt tccgtcgcct ccgccgtacg ccggatgggg
     4261 cccaccgcag ccgcccgtcc cgccggcacc accgccctac gccggaccac ccgggtatgg
     4321 acgaagcccg tacggtcacc ccccaccgca atgggggtat ccaggttggg ctcctcccgt
     4381 ccacagccgc cggcgtccgg tcgcgcccat cctgctggcc ctcgtcggca tcatggccgc
     4441 cggcttcttc gtgctcgtgc tcatcggcgc ggccctgcaa gacggcggca cccgcgcccg
     4501 cgtcgacgcg tctgctgtcg accaccggcc ggcgcgagcc gagaaccccg gttcggcgcc
     4561 ggtcgacccg agcgacgcca acgcggtggt acgacgcaac accctgtaca ccgagggtgg
     4621 tctcgcaaac ggcaactgtc ctgccaagga cctcggcgac gccggccgcc aggagcagac
     4681 cgccttctac cgggcgctga tggaatgcct caacgacgag tggcgcccgc tggtggagaa
     4741 cgccgggtac agctacgagg agccaggcct ggtcgtcttc gactctccgg tggacacgcc
     4801 atgcggcaac gcgtcgccgg agagcggccg aacgctggcc ttctactgcc cgggggactc
     4861 cgtgatgtac gccgacgtgc cgcagatgcg gcggttcttc ggcgatgacg acgtggccta
     4921 cgcgatcgtc atcggccacg agttcggcca ccacgtccag caggagacgg gaatcctctc
     4981 ggcgtacgac gacctcgcac atgccgactt cgagagccgg ctcgagctca atcggcgggt
     5041 cgagctgcag gccagctgca tgggcgggct cttcctgggc gccatcgcgg agtcgttccc
     5101 gatgagcgac caacgtctga gccggctgga gcaggtcgcc gggtcgttcg gcgacgagcc
     5161 gggcggcggc gacgacaagc gcgaccacgg ctcgggtgcg tccaaccggg cctggatctt
     5221 ccgagggttc gatgccaacg acgccgagac ctgtaacacg ttcgtcgtgc ctgctggcca
     5281 ggtggagtag gtgcaccggt cgggtgtggg tgggcgcggg ttcggacagt ggatcggcgc
     5341 ggtcatcgac gttccacccg cggtggtcga cgcggttgac gccttctgga ctggcgtact
     5401 cggttggacg gtcggacctg cctggcccgg ccatccggag ttccaccacc tcgaaccccc
     5461 cgctggtgac agccacgtcc tcgtccaggc gatcgacggg cccgcccgca tccacctcga
     5521 cctgtacgtc gccgacatcg acgccgaggc cgcccgcctg gttgcgctcg gcgccaccct
     5581 tggtgagcgg catgcgcact ggcgagtgct cagcagcccg accggtctgc cgttctgcgt
     5641 cgtgacctca gaccgcgcga gggagcggcc gagtgcgccg acgtggccgg acggtcatcg
     5701 cagccggctg gcccaggtct gcatcgacgt tccacacggt ggtctcgacg aggagacagc
     5761 gttctggcag gcggtgaccg gctggacgtt ccgggagtcc gacgtgccgg agttcgcggg
     5821 tcacctcgag ccaggtcccg gtggcagcat gcagctgttg gtccaggagc tcggggcaga
     5881 cgatccagcg ggcggctccc gagtccatct cgacctcggc accgacgaca tcgaggctga
     5941 ggtgtgtcga ctcgtcgagc tcggcgccga ccgagtccgt gacgaccgcg gttgggtcgt
     6001 gctgcgagat cccgccggca tgctgttctg cgtgacggtc cagtctccct ccgaagcgac
     6061 ccttccgccc gagaagcggg gactcgacga gcctggaacc cgctgatcga gcctaccgag
     6121 aagcccgctg atcgagcccg tcgagatcaa gggtcggggt ctcgacaagc tcgaccgccg
     6181 gagagtcgag gtcaagagcg aggcgtcgat caggagacgt cgacggagat cgtgtgccaa
     6241 ccggtggcgc cgttcgggac cacgtcggca cggtcgccgg tctgcgtgta tccactggcg
     6301 tcggtcgccc gaaccgagat gtcgtgctgc ccgggctccg ccgaccagag gtaagaccac
     6361 tgtcgccagc tgtcgatggt cggctcgatt gccagcttcg cctgctgcca gtcgccgtcg
     6421 ccgatgcgca cctccacccg gtcgattccg gtgtgctgcg cccacgcgac ccctgccacc
     6481 acgacctgtc cggcggacac gtgggcaccg tcatcgggga cgtcgatgcg cgactcggtc
     6541 ttgatcgggc cgcgttcaga ccacccgcgc tgcgtccaga acgcgctgaa ccggtcgaac
     6601 cggctgacct cgaggtcgac cacccacttg gtgcccgaga cgtacccgta caggcccggg
     6661 acgaccatcc ggaccggaaa gccgtgctcc agcgtgagcg gttgcccgtt catcgcgaca
     6721 gcgaacatcg cgtcacggcc gtcggtcagg accgagagcg gcgtaccggc cgtccagccg
     6781 tcagccgacg tcgacaggac cgcgtcggcg tcggggtgca ggccgacgtc ggccagcacg
     6841 tcggcgatcg gtacgccgga ccaggaggcg ttcgagatca ggccgccgcc gacctggttg
     6901 gacacgcagc acagggtgat ccatgcctgc tgcaggccgc gatcgagcag gtcctggtag
     6961 gtcatcgtca gctctcgctc gaccatgccg tggatgcgga gcctccactc ggcgggcagg
     7021 acctgtggga ccgccagtgc ggtgtcgatc cggtagaagt cctgctggct ggtgacccac
     7081 ggcgccacac cgtcgacacc gaggtctgcg ccgctcgggg ccgggcgcgc agtgatcggc
     7141 aggcgcagtc gatggcgggc cgcttcgacg gccgcgcgac tgcgggccag ccaccgtccg
     7201 gttgcgccga ccacgagagc gccggccgcc accacacctg cggtggcgag gaaccgtcgg
     7261 cgactcagat cctgttcgcg cgaggcgagg gccgtcgacg cgccgtcggc cggcgaagtc
     7321 gtgtcgatcg gtcctggcga gacgacgcgc accgctcggg gcgccaactg ggcaagcgcg
     7381 aacatcgcca ccgcgacccc cgcgatcgcg ggcacgtacc tgaccggcga cgacgtcagc
     7441 cgtgcgtgaa ccgccgtcgc gaggacgacc cccatgatga ggaagatcag ctcggccagt
     7501 atcaggttgc gcagggccag tacgccgacg atgctgcttg cgagagcgag cccgaggagt
     7561 gtcaacgaca cgagcagcgg cttgtcgttg tggccgacca gcgagatgac gtgttcgatc
     7621 acggcccccg gagtgagccg gatgaccgac tcggccagag ccacgacggg tgaggcccgc
     7681 tgatggagcc agccgctcag gagctcagac accgccagac cggcgccggc cgaaaccatg
     7741 ccgagcacgg cgccgatgac gagcctcgat gaccgggcag gtcgcgtcct gtcctccggt
     7801 gttgtccgga cctgcgacgt cataccttcg atggtgcctc accggtcgcg cgaaccggag
     7861 gcgacttcgg ccgtcccgcc acggggggtg accgtgccga cgttggcatg gagcgatccg
     7921 gcagggtggg ccatgatgct gccgtgacca gcgaacccgc ccccgcctcc atccgtgtcg
     7981 gcgtcggagt cgacgtgcat cccctcgtcg ccggccgcga gctctgggtc gccgggctgc
     8041 tctggccgga cgagcgtgtg ggtctgtccg gccactccga cggtgacgtc gtggcgcacg
     8101 cggcctgtga cgcgctcttc tccgccgccg ggttgggcga cctcggaagc cagttcggca
     8161 cgtcggaacc gtcctgggag ggagccagcg gggtggcgct gctcgcggag acagcgcggc
     8221 gcctccgtct ggcaggcgcc ggcatcggaa acgtcgcggt gcagctgatt ggcgacctgc
     8281 ccaagatcgg tccacgccgt gccgaggccg agcgggtgct gtcggacgcg gtcggcgcca
     8341 gcgtgacctt gaccgcgacc acctccgacg gactcggcct caccggtcga cgcgaaggac
     8401 tggcggcggt ggcgacggcg ctggtctacg tcggctcctg agcctcgtca cgagacacga
     8461 ccacttccgc cagcctcagg tcgagcgctg tggtcacctt gaacgcgagc acgtcacctt
     8521 ccacgatgac gacgtccacc cccagcgact cggcaaggct tgcgtcgtct gtcgcctggt
     8581 cgtgaccagc ccggatgtgg gcctgtcgga gtacctcggt tgcaaagccc tgcggtgtct
     8641 gcaccgctcg catggacgac cggtccatgg tgcggctggt gttgtcggtg gtcacctgtc
     8701 gcaacgtgtc gaccacgtcg acagccggta cgacggcctg cgcgcccgct cgcaaggcgg
     8761 ccaccacgcg ttcggtcacc gccgacgggg tcagcggacg ggcagcgtca tggacgagga
     8821 cgtacggcgt ggcccgctcc accgcggcga gcgcgagctc gaccgaccgt tgacgggtcg
     8881 ggccgccttc gacgacgtcg acgacgaggg gtacgtcggc gagggacgca cggatctcgt
     8941 cgatccactc cgcgggagcg gggacgacga tctggtcagc gtggcgctcg acactgcgga
     9001 cggcatgacg cagcaacgtg tcccgaccga gccgtcgcag cggcttgggt aggccggagt
     9061 cggtcggggc ggccgcgagc cggctgccgg agcctgccgc cgggacgatc gccgccacct
     9121 gactcacgtg cgtcagcatg ccagagcggc gtactcactc ggatgcgaca ccggccatgc
     9181 gggcccccac ccagacacca ggccgcttga ggttggctca cgttcctcgg gtcgggcctg
     9241 caccgcaagc acccagtctc catcgagggt ttgcgatgtt tacagcccat ctgggctgta
     9301 aacatcgcaa accgacaacg gcggacgaag aacggccccc gccgcaaagg acgggggccg
     9361 ctcccctact cagacgattc aggtggtcag gatgcgagca cctcgtcgag gatggtctcc
     9421 gccttgtcct cgttggtgtg ctcggccagc gccagctcgg agacgaggat ctggcgcgcc
     9481 ttcgccagca tccgcttctc accagcggac agaccacgct cgcgctcacg gcgccacagg
     9541 tcccgcacga cttcggcgac cttcaggacg tccccggagt gcagcttctc caggttggcc
     9601 ttgtagcgac gcgaccagtt ggtcggctct tcggtgtgct ctgcgcgcag tacgtcgaac
     9661 acccgctcga ggccgtctga gtcgacgacg tcgcggacac cgacgaggtc gaggttgcag
     9721 gcgggcactc gaacgacgag atcgttctgg gcgacaatcc ggaggacgag atagagcttg
     9781 tcctcacctt tgatattgcg ggtttcgata tcttcgatga ccgcggcccc gtgattggga
     9841 taaaccaccg tctcgccgac cgtaaaagtc atgtgtcaga tacccctttc cgggcatcaa
     9901 ggataccacg tcgcgggggt ctcaggaatc acgtttcccc tgctcagacc gtaattctgg
     9961 gcttgacaaa aggccgatct cgtgcgtgtc gggagtcctc caccatcgtt gcgcgatggt
    10021 ttcgctacgc tgagcctgcc tggcctcccc caggccgcca cagtcgcgtt gacgttcgcg
    10081 gtcggttcga ccattgccgt aggagggcac ccatcgtgga gctgcggact ccccgtcgca
    10141 acggctcggc tccagccgga aaccgcttgt ctgccaggcc ttctgcccgg tcgcggctgc
    10201 cccgacggct cgccatcgcc gcgctcgtcg cgacgttgcc cgtcctgacc gcgtgcgccg
    10261 cgaacttcag cgcgcagacg cagcagcagt accagcctgc cgtcggtacc gacgaccgag
    10321 acggtgacgt ctacgtcatc aacgccgtca tcgtcgccga ccaggccggc aacggcaccg
    10381 tcgtgacgac actgatcagc cagcagagcg acgactatct ccaagggttc tcggccgagg
    10441 acagcaaggg tggcgcgctg aagacgaaca agcttccggc gatcactggg tcccaggatc
    10501 ccaacgctcc gtcgaacggc atcgcgctgc cagcccaaca gtcggtgaag atccccgacg
    10561 acggcgccct gcaggtgtcc ggtgacacga tcacacctgg aaccttcgcc accctgacct
    10621 tgacattcgt cgaggcggcg ccggccacga tcgacgttcc cgtcgtaccc cggagcgggc
    10681 cctacatcgg gacccagatc aggccgatcg aaacgccgtc gacaagcgag tccacgcctg
    10741 cttcgtgacc gcgttcggct gcccgaggcg gtgcagctca gacgtgggcg ggagcgtcgt
    10801 acttgtaccc gagcccccgc acggtcacca ggaaccgagg atgcgccggg tcgggttcga
    10861 tcttcgcgcg cagccgcttg acatggacat cgagggtctt ggtgtcaccg acgtagtcgg
    10921 cgccccatac ccggtcgatg agctggcccc gggtgagcac acgcccggcg ttgcgcagga
    10981 acatctcgag cagctcgaac tccttgagcg ggagccggac ctcttccccc gacacggtga
    11041 ccacgtgtcg atcgacgtcc atccgtacgg tgcccgcctc gagcgcaacc ggtgtcaggt
    11101 cctgctcgga acctcggcgt aggaccgcac gaatccgcgc caccagctcg cgcggactgt
    11161 acggcttcgt gacgtagtcg tcggctccga gctccagccc gacgaccttg tcgacctcgg
    11221 cgtccttcgc gctgaccatg atgaccggga ccgacgacac ctgccggatc cgacgacaga
    11281 cctccgtgcc gggaagcccg ggcagcatca ggtcgaggag cacgatgtcg gcgccgcccc
    11341 gctcgaactc ctccagtgcc tccggcccgt taccggccag cgccacgtcg aagccctcct
    11401 tgcgcaacat gtaggccaac gcgtcgctgt agctctcttc gtcctcgaca acgagaacgc
    11461 gggtcacgag gcctcctcgg catgtgatcg ggaaacggag tggagtacgc cgtcccggcc
    11521 gtcggtgtcg agcccgtccg acagaccgac gccgagaccg atcggatcct gcgcgtcgac
    11581 gttcctgctc ggcagtgtca acgtgaacga cgaacccgcg ccctcgacgc tccacacgcg
    11641 cacgtcgccg ccatgggtcg ccgccacgtg tttgacgatg gacagcccca gcccggtccc
    11701 gccggtcgaa cgggcacgcg ccgggtccac tcggtagaac cgttcgaaga tccggtccag
    11761 gtcgtcggcc ggtatgccga ccccttggtc cgtcacgacg atgtcgacca tgtcgtcgtc
    11821 gtcctcgttg gctcgcgcac tcaccgtgac tttcgtgtcg ggagggctgt aggcgacggc
    11881 gttctccacc aggttgccga gcgcgacgag gagctgctca ccgttgccga gcacctggaa
    11941 cccgtgctca ccggcgaagg acagctcgac gcgcttgtcg tgcgcgtcga caccgacccg
    12001 gtcgatcgcc cgctcggcca gcgcgtcgac cgacaccggg tcgagctcgt cgagcgggtc
    12061 gtcaccctgc agccgtgaga gctcgatgat ctgctgcacg agccgggtga gacgctggct
    12121 ctcggtccgc atccggacag cgaatcgctc gacagcctgc gggtcctcgg cagcctcgcc
    12181 gacggcctcc gccaacaggt tcagcgcacc gaccggtgtc ttgagctcgt ggctgacgtt
    12241 ggccacgaag tctcgtcgga tggcctcgac gcgccgttcg cgcgtacggt cctccacgag
    12301 cacgaggacg agccgactgc cgaggggcgc gacccgggcg gcgacgtgcc gagacacggt
    12361 tcgcccccgg cgcaggacga gctcgtcctc acggatctgt ccgtcccggc gtacttgccg
    12421 cacgagctcc agcagctccg cgaccgcgac gcgggaatcc cggacgaggc cgagtgcgtg
    12481 cgcaggcgca ctggccttca gcacctggtc atcgggtccg acgaccagcg cggacgaccg
    12541 aagcacggac agtacggcgg ccacgcccgg cggcacgagt ggctcggcag gcggcgccga
    12601 cggcgagaca ctctggatgt gctcactgcg taggaacgcc aggaccgcga aggcaccgag
    12661 catcgccccg ataccgccgc ccagcagggc ggccgtcgac gagtccatgc ctcgatggta
    12721 cgcagccggc cacctgccgt tggcagtcca gaccgcccag ttgcccgcag tttccgcgag
    12781 cgttcatgct gtgttcacgt ccgaacgccc agccgttacc ccacgtccct acggtggtcg
    12841 ccatgcgagt gcagtaccag caacaactcg accacgtcct cagcgagctc gtcgtcatga
    12901 ccggcggcgt ccgcgaagcc ctgtcccagg cgaccaaggc gttgctccag gctgacgcgt
    12961 cggccgcaga gagcgtgatc agcggtgacc tggcgatcga cgatgcccgc gaacagatcg
    13021 agaacgagag cttcgagatc ctcgcccgac aggcgccggt cgccggcgac ctgcggatgc
    13081 tggtggcctc gttgcggatg gtggcagacc tcgagcggat gggcgacctg tcggtgcacg
    13141 tcgccaaggt cgcacggctt cggttcccga cgaaagcggt gccggacgtg ctggagccgg
    13201 tgatctccga gatggcgaag accgccgacg agatgatcgg cgcagcagcc gacatcgtgg
    13261 ccaaccggga cgtccgggcc gcccaggagc tcgaagaggc cgacgacgag atggaccggc
    13321 ttcgacgctc gatgttccgc acgttgctga gcgacgagtg ggatgccggt gtcgagccgg
    13381 cgatcgacat ggcgctgctg gggcgctact acgaacgtat cggcgaccac gccgtctcga
    13441 tggcccgccg catcgtcttc ctcgtcaccg gcgagaaccc caccgccctc ctctgacccc
    13501 tgccgaggtg gcaggtttcg ttgaacttcg gcggcgtgtc ggaccgcgat tgccacctcg
    13561 acgggccgtc gctgccctac cgcttccagt cctcgccaac cacctcgctt tggagtgact
    13621 cgtctaaggt cgagggatga gtgacgtccc gtaccgtctg ctgctcgtcc gccacggcga
    13681 gagcgagtgg aacgccaaga acctgttcac cggatgggtg gacgtggcgc tcagcgagca
    13741 ggggcggggt gaggcggtcc gagccgcgga gctgatcgct gagcgcgaca tcgtgccgga
    13801 tgtgctgcac acgtccgtgc tccgccgggc catcgccaca gcgcagatct gcctcgacga
    13861 gatcgaccgg cactggatcc cggtacgccg ctcatggcgc ctcaacgagc gccactacgg
    13921 agcgctgcag ggcaaggaca agaaggcgac gctcgaagag tatggcgagg aacagttcat
    13981 gctgtggcgt cgctcgtatg acacgccgcc accggagatc gcggacgacg acgagttcag
    14041 ccaggcgggc gacccgaagt acgccgcctt gccgccggag ctgcggccgc gcaccgaatg
    14101 tctcaaggac gtggtcgagc gcatgctgcc cttctggtat gacgcgatcg tccccgatct
    14161 cctcaccggt cagacggtga tggtggcagc ccacggcaac tcgctacgag ctctggtcaa
    14221 gcacctcgac gacatgtcgg acgaagcggt ggtcgggctg aacatcccga ccggtatccc
    14281 actgctctac gacctcgacc acgagctccg tccggtgacc cgcggcggaa cctatctcga
    14341 cccagccgcc gcggaggagg ccatcaaggc cgtcgccaac cagggccgct gaccgaacgt
    14401 cccagacaga acatcccgga gaagcgaaga tccgggccac cgtgacggca gtcgtcaggt
    14461 gacccggatc ttcgttcgtg ggtgcgttac cggtgcgcgg ccaggtatcc gttgagccgt
    14521 tcgatccgcc gctcgaggcg gcgctgacgg cgagcgagtc gagcggcgga gatcagctcg
    14581 gactcccgcg cctcggctcg cctggacagg ctgacttcgc gcgcgacgtc cgttgcggtg
    14641 agcatgactt cctccgtgtt ctaccggcat ctcgccggga ctctcgtgtc gggctgtggg
    14701 ctgtgatggg tcgctgggca gcttgttcgg agcgtaagag acggctccgc ttgctgcctc
    14761 ttacacgata gcgaccacca gacgatgacc ccaaaccgac acggcgtgat ccccgacacg
    14821 ctcgatctcg gtgaactccc aggtcagggc gctggcggcg ccggcgcgtc gtcgcgttcg
    14881 agccagccac ggaacgcctc gaggttcctg gtggactccc ctcgagacag ccgccactcc
    14941 cattccttgc ggatcgacga ggcgaagccg agctcgagga tcgtgttgaa ggactcgtcg
    15001 gcgtacgaca acaccgaacc gagcagccgg tccagctcgc ccgggctcac cgccgacaac
    15061 gcgagccgcc cgtcgagata gatgtcgccg ttgtggtcga cgccgaatgc gacgccgtac
    15121 agacgcaggt tgcggctcag cagccaccgg tagacgccct cgtggttctc gtccggattg
    15181 cgacacacga acgcgtgcac cgccaccgcg tggtcgccga ccgccaactg gcagggcgtg
    15241 gccagcttgc gctccccggg caggtcgaag acgaacacgc cgtcgcgggg ctcccgaacg
    15301 tcgatctcgg cgtctgcgag cgcccgcctg atcgtgtcga tcgccgtggc acgggcgact
    15361 gcgatgtcgt cggacgacga ggtcattcgg ccagcgcgag cgcgtcggcc cgcatcgaca
    15421 gcgccgcgtc ccggtaggcc tcgagggtca gctgcgcggt ctggccccag ccgaaccgcc
    15481 gggcgtgtgc agcgcagtgg gcctgaagcc ggcgacgcgc caggtcgtcg gtcagcagcc
    15541 actcgaaaac cgcggcgtac tgcgccggtt cgtgtccgtc gaccagtatt ccgctgacgc
    15601 cgtcgtcgac ggccgtcggc agtccgccga cgttcgctgc gacgaccggg gtgccgcagg
    15661 cctgggcctc gatcgcgacc agcccgaacg actcgttgta cgacggcacg gcgaccagcg
    15721 acgcggctcg gtagtagtcg acgagtcgcg cttgctcgac cggcgcgacg aatcggacga
    15781 tgtcgccgac acccagcgac gccgccaggt cgaccagacc atccggcgtt tccagaccgg
    15841 agcccgacgg cccgccgatg atcgggacga ccaaccggtc gcgcaggtcc ggccggcgat
    15901 cgacgagccg ggcggctgcc ttgaccacga cgtcaggtgc cttgagcggc tggatccgac
    15961 ccacgaacat gacgacgagc gcgtcctccc ggacaccaag ccgccgccgc gcgtccgtct
    16021 ggcttcccgg ccggaagacg cgcagatcca caccgggagt gatcacccgg agtcgctcgg
    16081 gttcggcggc gtacaggtcg aggagctggc gacgctcgtc cgcggtgttg gcgatgatga
    16141 ggtcggccgt ctcgaccacc tgctcctcgc cgatcacccg cgccatcggc tcgggcacgt
    16201 ctccgtcggc gagcaggtgg ttcttcacct tcgccgtcgt gtgcatggag tggacgagcg
    16261 ggatacccca acggtcgcgg gccaatgcgc ccacctgacc ggacagccag tagtgcgagt
    16321 ggatgatgtc gtagtagccg ggagcgtagg cggcctcggt acgcaagacg tctcgcgcga
    16381 acgtgcacag ctgccccggg agctcaccct tggtgagtcc ttcgaacgga ccggccacga
    16441 cgtggcgcac agtgaccccg tcggtcagct cgacggtcgg cgggaggcgg ccggacgtgg
    16501 cgcgggtgaa gatctcgacc tcgatgccac gcgcggcgag ctggcgcgag agctcgacct
    16561 cgtagacgtt cataccgccg gcgtcgccgg ttccgggctg gtccaacggt gaggtgtgga
    16621 cgctgagcat cgcgatccgg cggggcgtca gcgcccggtc gaagggaacg acacgggcca
    16681 gcgtcgcggt catgctgacc ctcctgtcgt cggtgcgtgg tccggaagat gacaacagtc
    16741 gtctagtctc ccgcatttcc ggtgtgctcg cgaatcgagc ggtcaggcat caggagaagg
    16801 gaggtgcgtc ggctccgacc ggctggtcag tgccaggatg gcgccatgtc tgaccctgca
    16861 caggctggac ctgacggacc gcgtcttgcc gtggtgaccg gagcgagcag cgggatcggg
    16921 gcagccgtgg cacgcgccct cgcccgggtc ggcttcggtg tcttctgcgc cgcccggcgg
    16981 ctcgaccgca tcgagtcgct ggccgaggag atcggcggcg aggcggtcgc ctgtgacgtc
    17041 accgacgagg cgcaggtcgc cgatctggca gctgcggtcg gcagcaggct ggacgtgctc
    17101 gtcaacaacg ccggtggggc gttcgggacc gagccggtcg ccgaggggcg ggtcgaggac
    17161 tggcgagcca tgtacgacgt gaacgtcatc ggaaccttga acgtcacgaa ggcgctgttg
    17221 ccggcgctga tcgcgagcgg tgacggcgtc ctcgtcaaca tgggctcgac ggccggccgg
    17281 gtcgcgtacg aaggtggtgg tggctacacg gcggccaagc acggcgtggc cgtgatgacc
    17341 gagaccttgc ggctggagct cgtcggccag cccgtccgca tcagcgagat cgcgccgggc
    17401 atggtcaaga ccgaggagtt cggactcgtc cggttccgcg gcgacgagga agcgcgagac
    17461 gcggtgtacg aaggcgtacc cgatccgctc tccgctgagg acatcgcgga cgcggtcgcc
    17521 tgggtggcca cccgtccgtc gcacgtcaac atcgacctga tggtcatgcg tccgcgggcg
    17581 caagccgccc agcacaaggt ctaccgggag acctgactcc atcatcccca gcgaatgtgg
    17641 tgcgccgagc gccatcggtc gagcatgccc tggtcaccct cgaccgtgat ttcgtcgccc
    17701 cggttccaca gcccgacgta gagctgtgcc gccgtgccga agaacaccgc cgccacctcc
    17761 gctccctccg tgccagggtg cagctctgac gtgaccggtg gctcggcaga caccgagacg
    17821 agccaggcgc ggtcgtggtc ggtcggacga acgagaatcg tgaactcctt tccggcgtac
    17881 aacctgctcg gccgtcgggt gatgaagccg cgcagcagct cgtcgatgcc gtcgattgcc
    17941 agctcgagat cgattgcgca ttcggctgcc gtggggatgg ctccgagccg gccggccaac
    18001 gcgtccaccg cgtggatcgt cgtctcgtgc gcctgccgcc gcgcccagaa ctcgcgcggc
    18061 gccggcgcgt cgttgaggaa cacccaggcg tcgaggtcgg ccggagcggc ttccagtgcc
    18121 cgcacgagcc cgtcgtattc ctcccggtag tagccgacga ggtcgggttg cgtcgttcgg
    18181 atcacgtcgt cgtcgagcgg ttcgcggtcg acgtctcggc gtacatgcgc cgcggcccaa
    18241 cggtgcgcca tcgccaggtg tgccaccagg tgcgcgatcg tccagtccgg gcaggtcggc
    18301 actggcgcgt cgaggccggc gagctcagcg cggtcggcga gtgccgggcc cgaacgccga
    18361 atggcctcga gatgtcggtc cagcgtcagc gaggtgggca tgtcccccat cttggcgaac
    18421 aggcggcacg ctcgcttccc tcggggcgcc cccatcgcaa gctcctcgtc tccatcgtgg
    18481 gtttgcgatg tttaaagccc aagcggcgtg taaacatcgc agacctccga tgggcacagt
    18541 gggtctcgga tgtggacttc gcaaacccac aacggccgtt gcggtggtca gagggagtgg
    18601 cggcgggcgc ggaggaagcg ccgttccgtc tcgttgtcgc tgagctcgat cgcgatgtcg
    18661 tacgccgctc tcgcctccgg ctgccggccg agacgtgaga gcacgtcggc ccgagtggcg
    18721 gcgacgtagg cgtaccgctc cagggttgga tcgagcgcga gcacgtccag ctcggcgaga
    18781 acggccggga gatcggcgcc cggcacgagg ctggtggcgg ccagccggtt gagcgccacg
    18841 accggactcg gccacgtcag cagcagggcg tcatacatcc gcaccacctg ccgccagtcg
    18901 gtggtttccc aggacggggc ggtcatgtgc agcccggcga tggcggcctg cagcgcgaac
    18961 cgtccaccgc cgtcgagcgc ctgcgtcgtg agcgccaggc cctcgtgcac gagcgtcgtg
    19021 tcccaccgtg agcggtcctg gtcggcgagc agcaccaggt caccggcagg accgagccgt
    19081 gccgccccgc gagcctgcgc gaagagcagc agggccagca gtccctgcgc ctccggctcg
    19141 tccggtagct gcgccaccag cagccgggcg agccgcagcg cgcggtcggt cagctcaggt
    19201 cgggtgagtg cgtcgccggt cgccgtgtgc ccggcggtgt acgcaaggtg gacgacggtc
    19261 agtaccgtgt ccagccgctc cgggagctgg tcgtcggtcg ggagtcggaa cggcacacct
    19321 gcggcggcga tcttctgttt ggcccgagtg accctggccg cgatcgtggc ctccttggtc
    19381 agcaggacct tgccgatctc ggctgtcgtc agtccgcaca tcagccgcag cgtcagcgac
    19441 agctgagcgt cccggccaag ggcggggtgg cagcaggtga agaccaaccg gagcgggtct
    19501 gccggcgcag tcaggtctgc atcgggcgac tcggaggtct cctccacgag cagtaacggc
    19561 agcttgcgcc gcagcgaatc ctcgcgccgg agccggtcga gcgctttgcg gcgagcaacg
    19621 gtcgtgagcc acccagccgg gttgtccggt atgccgtccg gccaggaccg gagcgcctgc
    19681 acgaaggcat cctgtgcgca gtcctcggcc aggtcgatgt cacgcgtgag gcgcaccgtt
    19741 gccgcaagga ccgttgccca gtggctgcgg tgcgcctcct cgacgttccg gacgacgtcg
    19801 cgcacgtcga tcacgggcac gcgtggcact tcagaccgtg accggatcgc gtcgaggtct
    19861 cgtgtcagct cggggcgtcg ccgctggtgt ccatcaccgg ccggacctcg acatggccgg
    19921 acggacagat gttggccagc tccagcgcct ggtcgaggtc acgtgcctcg acgaggtaga
    19981 acccgccgag tgcctccttg gtctcgatga aaggcccgtc ggtcgcgacc ggcttggcgg
    20041 acgggtcgct ccagtccttc gtgatcgtcg tggcggtcga gctgcgctcc agtgcgcgac
    20101 cttcgaggat ccgggcgccc gctgcttcca ccgcggtggc gaactcgccg tgcagcttca
    20161 tcgccgtctc ccagctctcc ggcgtcagct cgtcgtacca cttctcgtca tcgaagagca
    20221 ggaacatgta ctgggccatg gtggcctcct ccgtttcggg gtcgttgctg aactgacgaa
    20281 cggcggtgtg gcgattcgac aacctcgggc gggtcgtgtc cagtttcctc gctcggcggc
    20341 cgccacgaca gcgccgggca gcggcggcgt acgctctctg ctcatgccgg gcaccgacct
    20401 gacccccact tcacgcgcgg acctgcgcaa cgtcgcgatc gtcgctcacg tcgaccacgg
    20461 caagacgacg ttggtcgacg cgatgttgtg gcagtcaggt gccttcggcg agcatcagca
    20521 cgtcgacgag cgcgcgatgg acagcggtga cctcgagcgc gagaagggca tcacgatcct
    20581 cgcgaagaac accgcagttc gctacacccc agcatcgaag agcgatgctg ccgctgcgtc
    20641 ccagccccaa cgcgccgtca ccatcaacat catcgacacg ccaggccacg ccgacttcgg
    20701 cggcgaggtc gaacggggcc tgtccatggt ggacggcatc gtgctcctcg tggatgcctc
    20761 cgaggggccg ctgccgcaga cgcgcttcgt cctgcgcaag gccttgtccg cgaaactccc
    20821 cgtcgtcctg gtggtcaaca aggtcgaccg gcccgacgcc cgaatctccg aggtcgtcga
    20881 ggagacgtac gagcttttcc tcgacctcct cgacgacagc cacgaccagg acgcgctcga
    20941 cttcccggtg gtgtacgcct ccgccaaggc cggccgcgcc tccctcgagc agccggccga
    21001 tggcgacctg cctgactcac ctgacctgga gccgttgttc cggacgatca tcgagacggt
    21061 gcccgccccg acgtacgtcg acggcgcccc acttcaggca cacgtcacga acctggacgc
    21121 ctcgcccttc ctcgggcgac tcgcactgtg ccgcgtgcac gaaggaacgt tgcgcaaggg
    21181 ccagaccgtc acctggatca agcgggacgg gtcacagcag aacgtcaaga tcaccgagct
    21241 tctggtgacc gaggcgctgg accgcgtacc gggcgactcc gctgaaccag gtgacatcat
    21301 cgcggtcgcc ggcatccccg acatcaacat cggcgagacg ctcgccgatg tcgacgagcc
    21361 ggtcgcgctg ccgctcatca cggtcgacga gccggcgatc tcgatgacga tcggcaccaa
    21421 cacgtcgccg ctcgccggcc gagtcaaggg ctcgaaggtg acggctcggc tcgtccggga
    21481 ccggctcgat cgtgagctgg tcggcaacgt ctcgatcagg gtgcttgcga ccgagcgacc
    21541 cgacgcgtgg gaggttcagg gccggggtga gctcgcgctg gccatcctcg tcgagcagat
    21601 gcgacgcgag ggattcgagc tgactgtggg caagccgcag gtcgtggcgc gcgagatcga
    21661 cggcaaggtg cacgagccgg tcgagcggct caccatcgac tgtcccgagg agtacctcgg
    21721 caccgtcacc cagctgctcg cggtccggaa gggccggatg gagcagatgg tcaaccacgg
    21781 gaccggctgg gtgcggatgg agttcctggt gccggctcgt ggcctgatcg gttttcggac
    21841 cgacttcatg accgacaccc gcgggaccgg gatcgcgcac cacgtcttcg aggggtacga
    21901 gccgtggttc ggtgagttgc ggacgcgtcc gtctggttcg ttggtggccg accggaccgg
    21961 gacggcgacg tcgtacgcca tgttcaacct gcaggagcgc ggaagcctct tcgtcgagcc
    22021 cggcaccgag gtgtacgaag gcatgctggt cggcgagaac agccgttccg acgacatgga
    22081 cgtcaacatc acgaaggagc gcaagctcac gaacgtgcga tcgtccacgg ccgaggagct
    22141 cgagcggctg gtgccgccgc gccggctgtc cctcgaacag tcgctggagt tctgtcgcga
    22201 ggacgaatgt gtggaggtga ccccggaagc cgtccgcctc cgcaaggtcg tgctcgacca
    22261 acacgaacgc gccaagtccg ccgcccgttc ccgccgcacc tagtccacgt ccgtggtggg
    22321 tttgcctggt tagccgtgca tctggatggc taaccaggca aatctgccgc gggaggggag
    22381 gatctgcctc gctggctcag ttccgcgcat gtcacgtcag ggcgagttcc agtaccgcga
    22441 acgccgtcga gccgaacgcg accatccgtg cttcctcgac cgatgtcggc gtactccgca
    22501 acgtctcgac ggccgcgttc gccgcgtcct gcaacggcca gccgtagata cctgctgaaa
    22561 cgagcggaaa cgccacggtg cgtgcgccga gctcgtcggc gaccgcaagt gcgttcgcgt
    22621 agcaggacgt caacaaggaa cggtcagtct cgcccgcggc gtggttcggg ccgacgacgt
    22681 ggatgaccca gcgcgccgaa agccgtccgg ccgtggtcca gccggcgtga cccgtcgcca
    22741 gcccgttcgg gaagcgctcg atgcagtcct gcaggacagc gggaccacct gctcgatgaa
    22801 tggcgccgtc gacaccgcca ccgccacgca taccgcggtt ggcggcgttg acgacggcat
    22861 ccacctgctg ctcggtgatg tcaccctcga caagcgtgat ctccatgcct tcttcgtacc
    22921 ctgacccatc gagacagact ccccacccca tacaggtttg gggactcctc aggattctgt
    22981 ggcgccagag aaggtcgcag gcgggggcgt gctcgcccag ccccgcgtcg tgatgcgctg
    23041 gggtgggtcg gcaacgatga ggcggatcgt tcgtgcccgc ttgagcttgc cggccgccgt
    23101 tccgtccctc tcgggtgaat gttctgccgt gtggttggcg ggtttcggat atccgcggct
    23161 gaccggcctg ggttgcgatg ttcacatccg agactcacgg cgcccatcgc aggtttgcga
    23221 tgtttacaca ccttttgggc tgtaaacatc gcaaacctcg gccggccgag gtcaagcatt
    23281 gaggaaggcg gagatgcggc ggcggaccgc ggcgcggtgg ctccacaaga cgccgccgtc
    23341 gtcgtagatc tcgaggtccg cgtcgggaag cgccgctgcc aggtcgcgcg caaccgaggc
    23401 tgggtgcgcc ccgtcgccct cctggccgat caccagcacc ggacaccgca ccaccgccag
    23461 ttccgcacgg gacttcagcg gatactgcgg cggcagctcg cggagcgccc tggccacggt
    23521 tgtcgcggag agccggcgcg cctggcgtct cgcccacagc cgtacgtcag accggtctcg
    23581 tacgccgacc ggctgctcgg cgaccagcga ctccgccaga ctccgcagat cacgatgctc
    23641 gatcagcgcg gcccgagcct gcatgcggtc gatcgcgggg tcggttcgcg gctggtcgac
    23701 gctggcgggc aacaccacga ccagccgccg gaacgcgtcc ggcgtgtcca ccgccgaccg
    23761 caacagcgcg ccggccccga gcgacacccc gagggcctgg tgcgcgtcgt acgccgtccg
    23821 gaccgcatcg agctcggcag cagcagcggc gtacgtccac ggcgcctcag agccaccgga
    23881 agcgccgtga ccgcggaagt ggaagaagac ccgggtgccg gagaccccgg atccgaacgg
    23941 acgtgtctcg tcgatcgagc tggccagacc atgcgcgaac accgtgaccg ggagtcctga
    24001 accgttgacc agacactcga tcggcccgtc cgggccggcg atcaggggca tctgggtctc
    24061 aggcgtcagt agcgcttcat ggcccgcagc gtcggacgca cgtcggcgag gtagacgagg
    24121 gccgcggttg tgccgatcag gttgagcacg tgacgcgggt cgctgatcag gtagtgcagc
    24181 acgaggaaga tcacgagcag cagcagccag aacgccttgt tctgtttgtc ggcggccacg
    24241 taggagttgt ccttgtggaa caaggcgtcg atcagcgcga acgtcttcac cgcgaacagc
    24301 aggatccaga tcacgaacag gatcaacgtc tcgaggttga gtacggcgtc gacggcggca
    24361 gtactgctca cgcgacaagg gtaaaccgtc gtcggacaag caacagcccc gcagacgtgt
    24421 tctctgcggg gctgtcgtcc ggctcggtca gcgaccggcc cgccggggta cgtcaggcgc
    24481 cggacgtcgg cgcggtgtcc tcgccggacc tcggcgcggt gtcgccgccg gacatcggcg
    24541 cgctgtcgcc gccgatcttc ggagcgtcgt cgccgacctt cgttgcggcg tcgtcggcag
    24601 ccttcttcgc ggctgaggcc gtcttcttgg cgctggtcgt cgtcgacttg acggacgtct
    24661 tcgtcctggt cgcggacttc ttcgccgtcg acgcggtctt cttcgccgcc tcctgcgcgg
    24721 tcgaagcggt ctccgcagcc gccttcttgg cggtggtggc cgttcccttc gcctggctca
    24781 ccgtcgtcga cgcctggtcg tcgaggtcct gtgtcgactg ctggccgcgg actcggtcga
    24841 ccagctcctt gcccctgccg gcgagctcgc cgtacgccgt gaagcccttc gcaacagcgt
    24901 caccgagcgc tgactgcgcc ttcgtcggca gtgtcttgat ctggtccggt gcggctgaca
    24961 ccttggcctg cgcctgcttc tgcaacgcct tgatctgctc cggggtcgcc gccaccttcg
    25021 cctgggcctg gccgcgcagc gccttggggt cgatcttccc ggcgcgcgtg gtgatctcac
    25081 caccgacgtc gcgcagtttc ttcacggcaa ggtcgccggc gccgacgacg gcgttgagcg
    25141 caccggagtc ggcgatggcc ttctcgatgt ccttgcgggt cttgtcgaac gaggcagcgg
    25201 gtgccatggt gttcctctct cagatggttg gcggggctca cgagccggtc gctggatcag
    25261 gttgtggcgt tgccctgccg aaacgatgca tagatctcga gcagcacttg cttctgccgc
    25321 tcgttcaggt cgggatcgcc gagtacggcg gtctgtacgg ttgcggcgcc gcgttcgtcc
    25381 gggtcgagga ttcccgcccg gatgtagagc gtctccgccg agatgcgcaa tgccttcgcc
    25441 agctgctgga gcacgtccgc agacgggcgg cgcagaccgc gctcgatctg gctcaggtag
    25501 gggttggaca ccccggcctg atcggccagc tggcgcagcg tgagctgcgc gccgcgacgc
    25561 tggtcgcgga ggtagtcacc caggttctgc gccgtccccg cgacgctggc gcgtgcggtg
    25621 gcggtgaccc gggcggccgc cttgggcgca ccctcggtga gcggcctccc aaccttcagc
    25681 gtcgccatgg gttcatcctg ctaacaaaag ttagcaaatg caagcagccg gaacgtgggc
    25741 ccggtcacag ccggtggcct caattgcgga gaactggtgc cgcgccacgg cgtgtcgcgc
    25801 tgacaattcc cgcaattcgc ggagacccgg cagcgctgtg aacggtgagg aacggcagct
    25861 gggtcagaac gccgggcgca gctccccgac cggctcgccg ccgccgagca gcggcacgac
    25921 gccggtcgcg cgcagtgcct cgacgtcgac ggcgtcatcc tccatgacac ccatccgctg
    25981 cagtgccgcg gccatcacga caggtctggc gcgcggcgag ccgtcctcga ccttcagcgc
    26041 gaccgcgcga ccgtcaggaa gcgccagcgc gtagcagcac tccgcacccg ccttcgccat
    26101 cgctccgggc accgcacgca gcagcttggc ttcgtcccgg gtgctcccgg acgcgaactc
    26161 cgggtatgcc gcgaacgcct ctgcgacgcg ccgctccggg ctgccggcac cggcgaccct
    26221 gatcgagcgg aacgcgcgag ccagcccgag cagcgtggcg gagaacagtg gcgccccaca
    26281 gccatccacc ccgatgctga cgacgggttc accggtcagc tcggcgaacg ttacgcagat
    26341 cgcctcttgc agcgggtgct gcgggagccg gtaggtcgcg atgtcccagc cgcgctcgac
    26401 acacgtggcc agcatggccg cgtgcttgcc ggaacagttc atggcgatgg cggacttctc
    26461 cagcccggcg cggatgtacg ccgtcttcgc ggcgtcgtcg agcgggaagt ccggcggcgt
    26521 ctgcagtgcg gactcgtcca accctgccga cgacaggatc cggcgtacgc cgtcgatgtg
    26581 gaaggactcc cccgagtggc tggccgcggc cagcgccagc agctctccgt ccaggtcgag
    26641 gccgagccga agcatggcca gcgcctgcac cggcttgttg caggaacgtg ggtagatcgg
    26701 gctggtcacg tcaccgatcg accagtcgat cgaaccctcg gtgtcgagcg ccaccaccga
    26761 gccgcggtgg tgtccctcga cgaacccgga ccggacgatg tcgacgagga tctcggtgtc
    26821 agccacgaac cgtgagcgta cctgcgtcca gatcggccac ggcggcggga ccgcggtggc
    26881 acaatcgcac ccgtgtccac tcctgtcgag ggcaaggtcg gcgtgccgct gtggactccc
    26941 acgccgcgcg agctcgatga cctcgacctg ctgatcagcg gctgcttcgc accacccctg
    27001 cccgggttcg tggcgccgac cgatgactcg gcaccgatca cgttgcaggt ctccggaacg
    27061 gtcgccgaca tggtcatcgc cgtcgggcag gtcgacctgg tcgaccccga gggcgcaccc
    27121 ctggcgcgga tgacggtgac cgggacctgg cccaccgacg aacctgacgg gctcggtctg
    27181 gtcgggcctg tcgagcgcct ggcgcacccg gagttcgggc cgttccgccg cctgcacgtg
    27241 ccgccggcga cactccacga gacagtgccc gcggacacgc tgcttgcggt tccggtccac
    27301 cgggtgctca gcgggaggga catcgacgag atcgcgcggg ccgccaagga ccaggatcgg
    27361 atcgcagtcc tgctcgcctg tgtcggcgac ggcatgccac aggacatctc cggccccgcg
    27421 ctggtgcgcg ccacccttgc ggctgcaccg ttgatcggcg ccgacacagc cgtctgcgcg
    27481 gtcgcggcgg ccgatcacgg ctgggacgcc gcaccgggtg tccgctcgcc gaacgactgg
    27541 ctgctcgaca gcatcgcggc ggcgtacgcg acggcggcgt accgggtcga gcggcagccg
    27601 acgggggcgc tgcccgacgc ggtggccgcc atcgcgaccc gagaccggcc accgcgcgac
    27661 cggcaaggcg tcgtgatctt cttcaccggg ctgtcaggtt cggggaagtc cacactggcc
    27721 cgcgccctgg tcgaccacgt cctcgagcag ggcgatcgca cgatcacctc cctggacggc
    27781 gacgtggtcc ggcaccacct gtccaagggg ctcggcttca gccgggcgga ccgggagacg
    27841 aacatcttgc ggatcggctt cgtggcggcc gagatcgccc ggcacggcgg tgtcgccatc
    27901 tgctcgccga tcgcgccatt cgagtccacc cgttcggaag tacgccggat ggtggagcgc
    27961 gccggcgggg gcttcgtcct cgtgcacgtc tcgaccccac tggaggaatg tgagcgccgt
    28021 gatcgcaagg gtttgtacgc gaaggctcgg gccggcgaga tcccggactt caccggaatt
    28081 tcgtcgcctt atgaagaacc ggttgacgcc atcaaaatcg acacaactaa tagagatatt
    28141 gcagactgtc tcgccgagct gctcgacgtc gtgacgacgg aaggctggct tcacccgtag
    28201 ctccccaccg gagggttcat gttggacgcg tcccagttgt tggccgacag tgcgctgtcg
    28261 atcagcagca tcgaggtcaa gaccgcggta gcggccttcc ttgtcggtgt cgtcgtcggc
    28321 atcaccggca tgggcggtgg ggcgctgatg acgccggcgc tgatcttcct gggcgtgggg
    28381 gacgcctcca gcgtcgtcac cgcggatctc accgcggccg cggtctacaa gaccggcgga
    28441 gcggtcgttc acgcccgtgc aggatcaccg aacctcagac tggccggctg gctgatcgcg
    28501 ggctcggtcc catgcgcgtt cctcggcccc cacctggtgc actggttcgc accagaagcc
    28561 gacatcgaca cgttcctgaa gaaggcgatc ggcttcgcgc tgctgctggc ggcctcgacg
    28621 tacgcgctgc gcctgtacct cgagctgcgg cgccgcacca gtggacggcc gacgccgagc
    28681 ggtgaaccgg tgatccggcc gatcccgacg atgctgatcg ggggcttcgg tgggttgctc
    28741 gtcggcatca cgtccgtcgg ctccgggtcg gtcatcatgg tcgcgctgct gatgctctac
    28801 cccatgctgt ccgcgctgaa gctggtcggt accgaccttg tgcaggccgt gccactcgtg
    28861 ctgtcggcgg cgatctcgaa catcatggtg aacgggctcg actggagcat cgtgatcccg
    28921 ctcaccatcg gctcggtgcc aggttgcatc ctcggcgcca aggtcgcgcc aatgatgcgc
    28981 cagacctaca tccgtcgcgg gatcatcgtg gtgctgacga tgtccgggct tgcgttgctg
    29041 gacaaagccg ggtgggcgcc gctcgggtcg ggtgaggacg acacgcaccc ggtgttcatc
    29101 gccctggtag gagtcgcgtt ggtcttcctg gtgccgctgg tctggggtct gttgcgcatg
    29161 gagcgtgggc tgccgatgtt cgggacaccc acggtccagc agatcgagga tcaccaggcc
    29221 gacgtacccg atcggccgac gggcgaggcc gcaccgaacg ccgtcgagga gtccgatcga
    29281 acgctggagg aaccggctcg caagtcttcg gactagcgct tgcgagcggt cgcgctgccc
    29341 ctcatgcgcg ggttgcaggt cgccggtccg cactccgatc tgtgtcacgt agaccacagc
    29401 agtccgcgca accggccaaa tcacactgcc gaccaccgtt aggattggcg cgaatctcat
    29461 gcttgaggag cctttgcgta tgaccgtgca cgctgactac cgcctcagcc agctcgagga
    29521 gctggaggcg gagtcgatcc atatcttccg cgaagtcgcg gcggagttcg agaagccggt
    29581 gctgatgttc tccggcggca aggactccat cgtgatgatg cggctggccg agaaggcgtt
    29641 cttccctgcc aagatcccgt ttccggtgct gcaggtcgac acaggcttcg acttcccgga
    29701 ggtcctccag acgcgggaca actgggtcga ccgactcggt gtccgtctgg tcgtcgcctc
    29761 cgtcgagcag gccatcaagg acggcatcgt cgtcgacgac ggcaagacca gccgcaaccg
    29821 cctccagatc ggcaccctgc tgaacgccgt ggaggagaac gggtt
//
LOCUS       DATBFZ010000022         6399 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_1652329, whole genome shotgun sequence.
ACCESSION   DATBFZ010000022 DATBFZ010000000
VERSION     DATBFZ010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 6399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6399
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1652329"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..454)
                     /locus_tag="VH419_00710"
     CDS             complement(<1..454)
                     /locus_tag="VH419_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012921047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEY7042168.1"
                     /translation="MTRSGFLSNTGVSEREAEVLALVGKRLSNAEIASRLFISVRTVE
                     SHVSSLLRKLGMSDRRALADVAATVADADIDPAARPASPSLPSPLTSFVGRAAERAAL
                     AEVLAGHRLVTAVGPGGIGKTRLAISVAGDVAAGRRDGVWFVDLVRVTE"
     gene            759..1196
                     /locus_tag="VH419_00715"
     CDS             759..1196
                     /locus_tag="VH419_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042169.1"
                     /translation="MTAILGTWNPRLWQWDNIADAIVATASGRSVVDSWSVNNRRNGV
                     NPGDRFYLLKVGKHPRGLVGSGTILSEPFRAPHWDGTPGKTTKVIDVEFDRLIDPEHV
                     FPAAVLSEELTHTNWKPMSSGTTIHPADERPLESLWKDHLTQL"
     gene            complement(1269..1341)
                     /locus_tag="VH419_00720"
     tRNA            complement(1269..1341)
                     /locus_tag="VH419_00720"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1306..1308),aa:Arg,seq:acg)
     gene            1703..3574
                     /locus_tag="VH419_00725"
     CDS             1703..3574
                     /locus_tag="VH419_00725"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020578783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HEY7042170.1"
                     /translation="MTAETRTPTTADDRAGDPSPSGIPSNATTTHAGLQAWVDDIAAL
                     TRPDRVHWCDGSDEEWAKLTAELVSRGTLVQLNPDIKPNSYWAASDPTDVARVEDRTF
                     ICSVEEADAGPTNNWMAPGEMKATMTDLYRGSMRGRTMYVIPFVMGHLEAEHPMFGIE
                     ITDSAYVVASMRIMSRMGTHVLHKIEELGEDADYVPCLHSVGMPLEPGQDDVAWPCND
                     TKYISHFPEERMIWSYGSGYGGNSLLGKKCYALRIASAKARDEGWMAEHMLILKLTSP
                     QGSVKYVAAAFPSACGKTNLAMLAPTVPGWKVETLGDDIAWMRFGADGRLYAVNPEYG
                     FFGVAPGTNQKTNPNAIRTLERGNSMFTNVALTDDGDVWWEGMEHEPAKATSWKKEPW
                     TPDSDELSSHPNSRYCTPIKQCPILAPEYDDPKGVPIDAILFGGRRKTTIPLVTEARD
                     WVHGTFMGATLSSETTAAATGAVGVVRRDPMAMLPFIGYNAADYFNHWITIGKEHDNV
                     KLPRIYYVNWFRRDDDGGFLWPGFGENSRVLKWIVERIEGTAPAVETPIGHVPTVDAI
                     DIDGLDVTRDDIAKALEVDVEEWRAEIPQITEWFEKFGDKLPAVLWTELDGLKARLG"
     gene            complement(3742..4248)
                     /locus_tag="VH419_00730"
     CDS             complement(3742..4248)
                     /locus_tag="VH419_00730"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HEY7042171.1"
                     /translation="MGNAAGARRRRWSAGLPALLGIVVLVALSAVTAPSVSATNHSSG
                     DPSKAAPNRLEANLNGAKEVPGPGDSNGRGHAVIRLRPAAHKVCVNATWSRIGRPIAA
                     HIHRGGPKAQGPVVIDLSTAVTGGAHCKGGVPTRLINRLKAHPGRFYFNIHTVQFQGG
                     AIRGQLHR"
     gene            4360..5979
                     /locus_tag="VH419_00735"
     CDS             4360..5979
                     /locus_tag="VH419_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEY7042172.1"
                     /translation="MTVAGGTGGGAWARSLVSYARAHRPRSWRHAIAVVALYVTGWAV
                     VAVPVAYHLFMTDSRSSVVAGHDVVVSPTHDGWATFDLGALLPDVRYPRDQWLGVHID
                     VGATNVDSYDTLIDRYAVIASQPAGEVDKVTGLVRDMAYDAMVKGALVGLVGPVLWLL
                     VGRRRRTELWSALTTRRGLAITATGALVAGLVVWPFGDNGTSATVSGSNRWEPIAQLL
                     PETTIEGEAARLQVQGGLITTGTKRLIQSAFDTYGKSRTYYHDLADRAPALAAQLHQP
                     REDETVALVVTDRHDNIGMDQVSRAIGDAGGATILFDAGDDTSTGEQWEAFSLDSVAT
                     AFDGYQRYGVAGNHDNGSFVSDYLTDLGFTMLNGEPVKAADGIRLLGAPDPRSSGLGD
                     WTTVGGISLEEQEHLLADAACRADDEGKRIATLLVHDASQGGEALDRGCVDLVLGGHL
                     HVQAGPTIVRGETGSVGTTYTTGTTGGAAYAFALGSKLRRDAEVSLVTYRDGRPVGIQ
                     PVMISTTGVFSVEPYVPLPSTDSRDAQQADN"
     gene            complement(6014..6104)
                     /locus_tag="VH419_00740"
     tRNA            complement(6014..6104)
                     /locus_tag="VH419_00740"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6068..6070),aa:Ser,seq:gct)
     gene            complement(6230..6321)
                     /locus_tag="VH419_00745"
     tRNA            complement(6230..6321)
                     /locus_tag="VH419_00745"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6285..6287),aa:Ser,seq:tga)
BASE COUNT         1129 a         2148 c         2132 g          990 t
ORIGIN      
        1 gttccgtaac gcggaccagg tcgacgaacc acaccccgtc gcgacgcccc gcggcaacgt
       61 cgccggcgac actgatcgcc agccgcgtct tgccgatccc gccggggccg accgccgtga
      121 ccagccggtg accggccagg acctccgcca gcgcggcgcg ctcggccgcc cggcccacga
      181 acgacgtcaa cggcgacggc agcgacgggc tcgcgggacg ggcggcaggg tcgatgtcgg
      241 cgtccgcgac ggtggcggcc acgtcggcga gggctcggcg gtcggacatg ccgagcttcc
      301 tcagcagcga cgagacgtgg ctctcgaccg tccggaccga gatgaacagg cggctggcga
      361 tctcggcatt gctgagacgc ttgcctacca gggcgagtac ctcggcctcc cgctcggaga
      421 cgccggtgtt actgaggaac ccagatcggg tcatggcacc ggtcaggatc cgtggtcgtc
      481 ccgcggcgga tccataaccg ggatggatga gtaggaccat cgccaacacg gcagtgacgg
      541 catgccgaca cgatacgacc gatcctgccg acgaggggat cgcatcagcc acgccgtgtc
      601 cgcggcagcg gtcacggttg gtggtcgcgg tccgttagac gccgcgtcct ggcggcttgc
      661 ccttgcccgg cttcttgggt tggcgctcgg agggattcaa accccaccct tttgacccgt
      721 agcatggcga tcggctcgcc gctagattgc gagacgagat gacagccatc ctcggaacct
      781 ggaacccacg gctgtggcag tgggataaca tcgcggacgc cattgtcgcg actgccagcg
      841 ggcggagcgt cgtggactcc tggtctgtca acaatcgcag gaacggcgtc aatcctggcg
      901 atcgtttcta tctgctcaag gtgggcaagc atccacgtgg cttggtcggc agcggcacaa
      961 tcctcagtga gccttttcga gctccgcact gggacgggac gcccggcaag acaaccaagg
     1021 tgatcgacgt agagttcgac cgcctcatcg accctgagca cgtgtttccg gcggcagttc
     1081 tgtccgagga gctcacgcac accaattgga agccgatgtc gagcggcacc acaatccatc
     1141 ctgccgatga gcggccgctt gaatcgctct ggaaggacca tttgacgcag ctctgagcca
     1201 acgcaagcgc agtggtctcg gcgggagggt cgaaggggtg aagtccgctc tgaccgggac
     1261 tttgttagcg cgctcggagg gattcgaacc cccaaccttt tgatccgtag tcaaatgctc
     1321 tatccgttga gctacgagcg ccggttggtg ttgctggtgc tgtcgatcgg cgtacgacgt
     1381 cggctcatcc gcgtcgtgac gcgccgtgtg agtttagcgt gacgccgtac gccggatgaa
     1441 atcgtgcgag gtcgccatcg cagactccga gttcacatcg cagatctgcg atgcgaacag
     1501 gccgggcggg ctgtaaacat cgcaaaccca ccacagtcac ccgcagaccc ggatggcgga
     1561 gggtatcgcg gcgggcgtac ggtggctgca accgcccaca gaaaccggac tgtgagcgcc
     1621 catatccaca tcaatccgcc gttggagacc ggcggccggt ggttcctatc caccgcgatg
     1681 cgcgagaggg aacaatccag gcatgaccgc ggagacacga accccgacga ctgccgatga
     1741 ccgggccggt gatcccagcc cgagcggcat cccaagcaac gcgaccacga cccacgccgg
     1801 cctgcaggcg tgggtcgatg acattgcggc cctgacacgt cccgaccgcg tccactggtg
     1861 cgacgggtcc gacgaggagt gggccaagct caccgcggag ctcgtctccc gcggcacgtt
     1921 ggtgcagctc aacccggaca tcaagcccaa ctcctactgg gctgcctccg acccgaccga
     1981 tgtcgcacgc gtcgaggacc ggacgttcat ctgctcggtc gaggaggccg acgccggtcc
     2041 caccaacaac tggatggctc cgggcgagat gaaggcgacg atgaccgacc tgtaccgcgg
     2101 gtcgatgcgc ggccgcacca tgtacgtcat cccgttcgtg atgggccacc tcgaggccga
     2161 gcacccgatg ttcggcatcg agatcaccga ctccgcctat gtcgtcgcct cgatgcgcat
     2221 catgtcgcgg atgggaaccc atgtgctgca caagatcgaa gagctcggcg aggacgccga
     2281 ctacgtcccc tgcctgcact cggtcggtat gccgctcgag cccggccagg atgacgtggc
     2341 ctggccgtgc aacgacacga agtacatcag ccacttcccc gaggaacgga tgatctggag
     2401 ctatggctcc ggctacggcg gcaactcgct gctcggcaag aagtgctacg cgctgcggat
     2461 cgcgtcggcg aaggcacgtg acgagggctg gatggccgag cacatgctca tcctcaagct
     2521 cacgtcgccg cagggcagcg tcaagtacgt cgccgcagcg ttcccgagcg cctgcggcaa
     2581 gaccaacctc gccatgctcg cgccgacggt gcccggctgg aaggtcgaga cgctcggcga
     2641 cgacatcgcc tggatgcgct tcggtgcgga cggccggctg tacgccgtca acccggagta
     2701 cggcttcttc ggtgtcgctc ctggcacgaa ccagaagacc aacccgaacg ccatccgcac
     2761 gctcgagcgc ggcaactcga tgttcacgaa cgtcgcgctg accgacgacg gggacgtctg
     2821 gtgggagggc atggagcacg agcctgccaa ggccacgtcg tggaagaagg agccgtggac
     2881 accggactcc gacgagctct cgagccaccc aaacagccga tactgcacgc cgatcaagca
     2941 gtgcccgatc ctcgcacccg agtacgacga ccccaagggt gtgccgatcg acgcgatcct
     3001 gttcggcgga cgacgcaaga ccacgatccc tctcgtcacc gaggcgcgtg actgggtgca
     3061 cggcacgttc atgggtgcca cgctgtcgtc cgagacgacg gcagcggcga ccggagcagt
     3121 cggcgtcgta cgccgcgacc cgatggcgat gctgccgttc atcgggtaca acgccgccga
     3181 ctacttcaac cactggatca cgatcggcaa ggaacacgac aacgtgaagc tgccgcggat
     3241 ctactacgtc aactggttcc gccgcgacga cgacggcggt ttcctgtggc cgggcttcgg
     3301 cgagaacagc cgcgtgctca agtggatcgt cgagcgcatc gaagggacgg cgcccgcagt
     3361 cgagacgccc atcggtcacg tccccaccgt cgacgcgatc gacatcgacg gcctcgacgt
     3421 cacccgcgac gacatcgcga aggcgctgga ggtcgacgtc gaggagtggc gtgcggagat
     3481 cccgcagatc accgagtggt tcgagaagtt cggcgacaag cttccggcgg tgctctggac
     3541 cgagctcgat ggcctgaagg ctcgcctcgg ctgactgtga attgcctcgg ctgattgtga
     3601 attgcgggaa cctgaggtcg gcaggcaggt gcgtgtcgta cgtttcccgc aattcgcaga
     3661 gaccccaggt gacgggacga ggcccccact ggcagctgtg gaggcctcgt catcggcggt
     3721 cgatggcagt cgatccgccg atcagcggtg cagctgtccc ctgatcgcac caccttggaa
     3781 ctgcacggtg tggatgttga agtagaacct tcccggatgc gccttgagcc ggttgatcag
     3841 gcgagtcggt acgcccccct tgcagtgcgc accacccgtg acggcggtcg acaagtcgat
     3901 caccactggt ccctgagcct tcgggccgcc ccggtggatg tgcgccgcga tcggccggcc
     3961 gatccgtgac cacgtggcgt tcacgcacac cttgtgggcc gccgggcgaa gcctgatcac
     4021 cgcatggccg cggccgttcg aatcgcccgg accgggcacc tccttggcgc cgttgaggtt
     4081 cgcctcgagc cggttcggtg ctgctttgct cggatcgccg gaggaatggt tcgtcgccga
     4141 cacactcggc gccgtcaccg ccgacagcgc caccaggacg acgattccca gtagagccgg
     4201 caaccctgcg ctccaacggc gtcggcgtgc acctgctgca ttgcccatcg cggcctcctc
     4261 gcggcatctc ccctatcacg aaggctcgac ccggccaccg caccggtcaa cctgtacaag
     4321 agggcattgg acctcccgtt ccgtccgtac gctggaaacg tgaccgtggc tggtgggacc
     4381 ggaggaggcg cctgggcgcg cagcctggtg tcgtatgccc gcgcacaccg gcccagaagc
     4441 tggcgacacg ccatcgcggt cgtggccttg tacgtgacgg gctgggcggt ggtcgcggtt
     4501 cctgtcgcct accacctgtt catgaccgac tcgaggtcga gcgtcgtcgc cggtcatgac
     4561 gtcgtcgtct cgccgacgca cgacggctgg gcgacgttcg acctcggcgc gctgctcccc
     4621 gacgtccgct acccgaggga ccagtggctc ggtgttcaca tcgacgtcgg tgcgaccaac
     4681 gtggacagct acgacaccct gatcgaccgg tacgccgtga tcgccagcca gcccgcaggt
     4741 gaggtcgaca aggtcaccgg gctggtccgt gacatggcct acgacgcgat ggtgaagggc
     4801 gcgctcgttg gtctcgtcgg acccgtcctg tggctgttgg tcggacgtcg acggcgtacc
     4861 gagctgtggt cagcactcac cacgcgccga ggactcgcga tcaccgcgac cggtgcgctg
     4921 gtcgcggggc tggtcgtgtg gcccttcggt gacaacggca cgtcggccac ggtgtcgggc
     4981 agcaaccggt gggagccgat tgctcagctg ctcccggaga cgacgatcga gggcgaggcc
     5041 gcgcggctgc aggtccaggg tggcctgatc accaccggga cgaagcgact gatccagagc
     5101 gcgttcgaca cctacggcaa gagccgaacg tactaccacg acctcgccga ccgagcccca
     5161 gcactcgccg cccagctcca ccagccccgc gaggatgaaa cggtggcgct cgtcgtgacg
     5221 gatcggcacg acaacatcgg catggaccag gtcagccgtg cgatcggcga cgccggtggt
     5281 gcgacgatcc tcttcgatgc gggcgacgac acgtcgacgg gcgagcagtg ggaggcgttc
     5341 agcctcgact cggtcgcgac ggcgttcgac ggctaccagc gatacggagt cgccggcaac
     5401 cacgacaacg gctcgttcgt gagcgactac ctcaccgatc tcggcttcac catgctgaac
     5461 ggcgagcccg tcaaggcggc cgacgggatc aggctgctcg gtgcaccaga cccccgcagc
     5521 agtggcctcg gcgactggac gacggtcggc ggcatctccc tggaagagca ggagcacctg
     5581 cttgccgatg ccgcgtgccg tgcggacgac gaaggcaagc gcatcgccac actcctcgtg
     5641 cacgacgcca gccagggtgg tgaggccctc gaccgtggtt gtgtcgacct cgtgctcggc
     5701 ggccacctgc acgtccaggc cggacccacg atcgtgagag gggagacggg gagcgtcggg
     5761 acgacgtaca cgactggcac gacgggtgga gcggcgtacg ccttcgcact gggcagcaag
     5821 ctgcggcggg atgccgaggt ctccctcgtc acctaccggg acggtcgtcc ggtcgggatc
     5881 cagccggtga tgatcagcac gactggggtg ttctccgtcg agccctatgt gccattgccc
     5941 tcgaccgaca gccgggacgc ccagcaggcc gacaactgac ccggtcgtgc cctcccgcgc
     6001 ttgcaggagg gtgtggcgga ggtggcggga tttgaacccg cgagagggga tcaccctcaa
     6061 cccgcttagc aggcgggcgc actaggccac tatgcgacac ctccacggcg gcacagccag
     6121 atgctggcgc cgccacagag taccggccca gcagccgccg actccaatcg gtgtcgggtg
     6181 gcgcagacgg cgcgggcgag ccagagccgg ccatgcggtc cgcgagactt ggcggaggag
     6241 gcgggatttg aacccgcggc tgacatctct gccagcgacc gctttcaagg cggttccgat
     6301 cggccactcc ggcacccctc cgggcacgac ccgcgcaggg ccgacaccgt gagggagtct
     6361 aggtggaccg ggtccgcaca gagatagtcc ccgacgcaa
//
LOCUS       DATBFZ010000023         3181 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_2966211, whole genome shotgun sequence.
ACCESSION   DATBFZ010000023 DATBFZ010000000
VERSION     DATBFZ010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 3181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3181
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2966211"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..768)
                     /locus_tag="VH419_00750"
     CDS             complement(<1..768)
                     /locus_tag="VH419_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020389651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HEY7042173.1"
                     /translation="MSSVVTFAPTVLDVGSSFRDTVLNGPVLLAIPVALIAGTVSFFS
                     PCVLPLLPGYLSYITGLSGADIVTNGSTGVRGRMVTGTLLFILGFSFVFVAVGGAFGQ
                     VGATLIEHQKTLTRVLGIVTIILGLVFAGVVPWMQRDVRIHQVPAVGLGAAPLLGVLF
                     GLGWTPCIGPTLGAVLSLSGTYGASPTRGAFLTFVYCMGLGIPFVVAAVSYRKMMTVV
                     GFVRRHQLAVMRFGGGMLVLVGLLLVSGAWDAMIVNMK"
     gene            complement(815..1438)
                     /locus_tag="VH419_00755"
     CDS             complement(815..1438)
                     /locus_tag="VH419_00755"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEY7042174.1"
                     /translation="MELEQQLMGSTPRRRLRRTRTSAALAAAGIVVVIATSGCTTSQG
                     DQTDNKGYVSGDGSITVIDAADRKQAPTLEGDKLGGGTLDTADYAGQVVVLNVWGAWC
                     GECRAEAEDLVQASRTLAKDDVAFVGLDTRDRESLALSYVRRFDVSYPSLVDQDSKLL
                     LKFYDVVSPNAIPSTLVIDASGNLAAIINGPTTSDTLVDLVRDVQKT"
     gene            complement(1486..2172)
                     /locus_tag="VH419_00760"
     CDS             complement(1486..2172)
                     /locus_tag="VH419_00760"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020392280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY7042175.1"
                     /translation="MSEAATSDSPSTEPVTTTVHLLRHGEVDNPAGILYGRLPGYHLS
                     ELGQQMAERVAKTVTGRDITHLVASPLERAQETARPTAEALGLEIVTDERVIESDNVF
                     EGKKFGPGDGALRRPSTWRHIYNPFRPSWGEPYKDVVVRMLAAMHDAREAAAGHEALI
                     VSHQLPIWIVRSFIEGRRLFHDPRKRECSLASLTSFTYTDDRITVVAYSEPAADLVPV
                     KAAKKFTAGA"
     gene            complement(2169..>3181)
                     /locus_tag="VH419_00765"
     CDS             complement(2169..>3181)
                     /locus_tag="VH419_00765"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017933162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEY7042176.1"
                     /translation="QVRLVSSGTEATMSAVRLARGFTGRDLVVKFAGCYHGHVDALLA
                     AAGSGLATFGISTTPGVPDASTAATIVLPYNDLQAVEQTFAEHGDRIACVITEAAAGN
                     MGAVAPVDGFNAGLSRLCTEHGALFVSDEVMTGFRASRAGYWGIDGAVEGWTPDLMTF
                     GKVMGGGFPAAAFGGREDVMAQLSPVGPVYQAGTLSGNPIATTAGVTTLRLATDDVYT
                     RLDTAASTIMRLAGEALSAAGVPHVMQAAGNLFNAFFVDPSEVPVVRNFDDASRQAQY
                     RFRAFFHAMLDRGVYLPPSAFEAWFVSATHDERAIDRIAAALPAAANAAAAATPDQEE
                     PR"
BASE COUNT          547 a         1081 c         1086 g          467 t
ORIGIN      
        1 cttcatgttc acgatcatcg cgtcccaggc gccggacacg agcagcagcc cgacaaggac
       61 gagcatgccg ccgccgaatc tcatcaccgc gagctggtgg cgccggacga agccgaccac
      121 ggtcatcatc ttccggtagg acaccgcggc cacgacgaac gggatcccga gccccatgca
      181 gtagacgaac gtgaggaacg cgccacgcgt cgggctggcg ccgtacgtcc ccgacagcga
      241 cagcacagca ccgagggtcg gaccgatgca cggcgtccac cccagtccga acaacacacc
      301 gagcaacggg gcggccccga gtcccacagc cggcacctgg tggatgcgga cgtcccgctg
      361 catccacggc accaccccgg cgaagacgag cccgaggatg atcgtgacga tgccgagcac
      421 gcgggtgagc gtcttctggt gctcgatcaa cgtcgcgccg acctggccga aggccccacc
      481 cacggccacg aacacgaacg agaagccaag gatgaacaac agcgtgcccg tcaccatccg
      541 accgcgaacg ccggtgctcc cgttggtgac gatgtcggcg ccggacaacc cggtgatgta
      601 ggagaggtag cccggcagca agggcaggac gcacggcgag aagaacgaca ccgtgccggc
      661 gatcagcgcg accgggatcg ccagcagcac cggcccgttg agcaccgtgt cacggaacga
      721 gctgccgacg tcgagcacgg tcggggcgaa cgtgaccacg gagctcacga catcagagac
      781 tgcggcaggg gaagacacga gagccaccgg acagtcaggt cttctgcacg tcccgcacca
      841 ggtcgacaag ggtgtctgac gtggtggggc cgttgatgat cgcggcaaga ttgccggagg
      901 cgtcgatgac gagcgtggac gggatcgcgt tcggggagac cacgtcgtag aacttcagca
      961 gcagcttgct gtcctggtcg accaggctgg ggtaggacac gtcgaaccgg cgtacgtagg
     1021 acaatgccaa cgactcgcgg tcccgggtgt cgagaccgac aaaggccacg tcgtccttgg
     1081 cgagcgtgcg cgatgcctgg acgaggtcct cggcctcagc ccggcattct ccgcaccagg
     1141 cgccccagac gttcagcacg acgacctggc cggcgtagtc cgcagtgtcg agcgtgccgc
     1201 ctccgagctt gtcgccctcg agggtgggtg cctgcttgcg atcggccgcg tcgatcaccg
     1261 tgatcgaccc gtcgccgctg acatagccct tgttgtcggt ctggtcgccc tgcgacgtcg
     1321 tgcagccgga cgtcgcgatc accacgacga tccctgccgc ggcaagcgca gcagaggtgc
     1381 gggtacgccg aagacggcga cgcggcgtcg atcccatcag ctgctgctcc aactccatga
     1441 tctgctgcgc gcctctcggc gatcgagatt cggaggcacc accgctcagg cgcctgccgt
     1501 gaacttcttg gcggccttga ccgggaccag gtcggcggcc ggttcggagt acgcgaccac
     1561 ggtgatccgg tcatcggtgt acgtgaacga ggtgaggctg gccaggctgc actcacgctt
     1621 gcgcgggtcg tggaacagcc ggcgtccctc gatgaacgac cggacgatcc agatcgggag
     1681 ctggtgcgac acgatcaacg cctcgtgccc ggccgctgcc tcccgggcgt cgtgcatcgc
     1741 ggcgagcatc cgcaccacga cgtccttgta gggctcgccc caggacggac ggaacgggtt
     1801 gtagatgtgc cgccaggtcg agggccgtcg cagggcgccg tctcctggac cgaacttctt
     1861 gccctcgaac acgttgtcgg actcgatcac ccgttcgtcg gtcacgatct cgagcccgag
     1921 cgcttccgcc gtcggtcgcg cggtctcctg cgcacgctcc agaggcgagg ccaccagatg
     1981 cgtgatgtcg cgtccggtca cggtcttcgc gacccgttcc gccatctgct ggccgagctc
     2041 gctgaggtgg tagccgggga ggcggccgta gaggatcccg gcagggttgt cgacctcccc
     2101 gtgtcgcagc aggtggaccg tggtcgtgac gggttccgtg ctgggggagt cgctcgtcgc
     2161 ggcttcgctc atcgtggttc ttcctggtcg ggggtggcgg ctgccgcggc gttggcagct
     2221 gccggaaggg cagcggcgat ccggtcgatc gcccgctcgt cgtgcgtcgc gctgacgaac
     2281 cacgcctcga acgcgctggg cggcaggtag acaccccggt cgagcatcgc gtggaagaaa
     2341 gctcgaaagc ggtactgcgc ctggcggctc gcgtcgtcga agttccggac gaccggcacc
     2401 tccgaaggat cgacgaagaa cgcattgaaa aggtttcccg cggcctgcat gacgtgaggc
     2461 acaccggccg cgctcagcgc ctcgccggcc agccgcatga tcgtcgacgc cgcagtgtcc
     2521 agccgggtgt acacgtcgtc ggtggccagg cgcagggtcg tcacgccggc ggtggtcgcg
     2581 atcggattgc ccgacaaggt gccggcctgg tacaccggcc cgaccggcga cagctgcgcc
     2641 atcacgtcct cacgaccgcc gaacgccgcc gccggaaatc ccccgcccat caccttgccg
     2701 aaggtcatca ggtccggtgt ccagccctcg accgcgccgt cgatgcccca gtagccggcg
     2761 cggctggcgc ggaagccggt catcacctcg tcgctgacga acagggcgcc gtgttcggtg
     2821 cacagtcggg acagaccggc attgaaaccg tcgacaggag cgactgcccc catgttcccg
     2881 gcagctgcct cggtgatgac gcaggcaatc cggtcaccat gttcggcgaa cgtctgctcg
     2941 acggcctgca ggtcgttgta cggaagcacg atcgtggcgg cggtcgatgc gtccggaaca
     3001 ccgggtgtcg tcgagattcc gaacgtcgcg agcccggacc ccgctgcagc gagcagcgcg
     3061 tcgacgtgcc cgtggtagca gccggcgaac ttcacgacca ggtcgcggcc ggtgaagccg
     3121 cgggcgaggc ggaccgccga catcgtcgcc tcggtgcccg aggagacaag ccgcacctgg
     3181 t
//
LOCUS       DATBFZ010000024         1715 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_1653627, whole genome shotgun sequence.
ACCESSION   DATBFZ010000024 DATBFZ010000000
VERSION     DATBFZ010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1715
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1653627"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(48..482)
                     /locus_tag="VH419_00770"
     CDS             complement(48..482)
                     /locus_tag="VH419_00770"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY7042177.1"
                     /translation="MVYLPLLLDLADPGEQGGETVLVVEDDPDTLSVVVDCLQEEGFT
                     VTTATNGVEALERACHCVPDAIVLDLCLPVMSGPEFLQAWRRTAPTQPVPIIAMSGLN
                     RDVSADELGVQAWLPKPFAVDALIHAVAAAVSRGGVHHAPLR"
     gene            1450..>1715
                     /locus_tag="VH419_00775"
     CDS             1450..>1715
                     /locus_tag="VH419_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042178.1"
                     /translation="MFAGRRSRWILVSLVLLGVAGVAVVGGVQYGPAVVSQLTGADGS
                     LFSGGNPDLTGADATVGSDVVAGATATPSPRGGRQVVPARRGPIS"
BASE COUNT          285 a          587 c          549 g          294 t
ORIGIN      
        1 gagcaccagc tccttcggcg tttcctgggg ctttcgggct cgtcgagcta ccgtaagggc
       61 gcatggtgca cgccaccacg gctcaccgcg gctgctaccg cgtggatgag cgcgtcgaca
      121 gcgaacggct tcgggagcca cgcctgcaca ccgagctcat cggcgctgac gtcacggttg
      181 agcccagaca tggcgatgat cggcaccggc tgcgttggcg cggtccgtcg ccacgcctgc
      241 aggaattcgg ggccactcat caccggcagg cacagatcga gcacgatggc atccgggacg
      301 caatgacacg ctcgctccag cgcttcgacc ccattcgtcg cggtggtcac cgtgaacccc
      361 tcctcctgga ggcaatcgac gacgacgctc aacgtatcgg ggtcatcctc gacgacgagg
      421 acggtttcac caccctgctc gcctggatca gcgagatcca gcaggagcgg cagatagacc
      481 atgtcgtagc aggtctgaca cagggtgagc gtggcctttc ctccgaaagg tgccgggaca
      541 acgaccgctg tcgtccgggc taccggcccc tcgcagttcc agcactccga tgtactgtgc
      601 tctcgcaagg ttggcgccat tgttgactct ccttgctcgt ggtaccgcgc attcggcgtc
      661 gacagggcat gagccccggg gctacctggc agttggcgag gaagtcctgg ctctcttgca
      721 ggcggcgcaa ctggcgtcgg agcgcttgtc gcggttggca gctacgacag cgtgtcaccc
      781 gcggcctgcg atgacgcgta cgaccagcgg atgaaagcaa gaacgagcac aatggcgcga
      841 acgggaccat gtccggcgcg cgctggcgca tgacgtcgcg actaccgccg catcgcagat
      901 gtacgggcaa catcagcatt ctctgctggt ttccgccgac ccgtcggtgg ctcgctccac
      961 ggcacgagca tgtcctggac cattcccccg atcgcatggc accctggctc tcagcgcccc
     1021 tctggccggc tggccaaccg cgtaacccgg atggatcatc gatccgggcg actgtggcca
     1081 gtatatagta agtatgggtc agtttcccat acagacatgc gcatctgtga tcgtgctccc
     1141 atccgcatat gctacgtgaa caccagacag cgaacgtcgt cagatcctgt gccaggttga
     1201 gcggcgctac gacctcggca cgaacgtgcc cggcacgaat cgcccggccg tgggctctca
     1261 ggcttgccaa cggcgggcgc gcaggcgccc ggtgccgcgc tgccgacgag tctcgacgga
     1321 tgctcaaccg ggccacaatc accgctggcg cggccctcaa cttccgaccc gtacgatgat
     1381 gcgacagaca ccccgcctca ccgaggcgac ggtgctgcat cacccggacg tgccgaggag
     1441 gttgtgtcca tgttcgcggg acggaggagc cgctggattc tcgtcagcct cgtcctcctc
     1501 ggcgtcgcag gtgtcgccgt cgtcggtggc gtccaatacg ggccggcagt tgtgtcgcaa
     1561 ttgaccggcg cggacggctc gctcttctcc ggcggcaacc cggacctgac gggggctgac
     1621 gccaccgtcg gcagcgacgt tgtcgccggg gcgacggcaa cgccctcgcc ccgtggtggc
     1681 cgccaggtcg tgcccgcacg gcgtggcccg atctc
//
LOCUS       DATBFZ010000025         2241 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_1328133, whole genome shotgun sequence.
ACCESSION   DATBFZ010000025 DATBFZ010000000
VERSION     DATBFZ010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1328133"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..973)
                     /locus_tag="VH419_00780"
     CDS             complement(<1..973)
                     /locus_tag="VH419_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042179.1"
                     /translation="MPAAARRRRTPFDVRRRTAIAAAAVLVLLLAGCTGHRDTGVHPQ
                     PQPDQSPPTATAGASGVPATRTIADTGGAPNLSALLNYPRVFAALAVVPENAAEISFT
                     DVRSVKERLGFASLTSNSAHAEHVAFWRRAEADGSMFNGSALNAFERTMSRDFGWTGD
                     DVDWEVDFATVETGCLRSMLCSTGRGRVFGLRPGLDWSVVTDSLARSGFTPDITPNGS
                     LVNRTRLAPFQRVLPIAGLHAVATGNSRGLARIGAVLEGAPTFGRRLANFSRSLPTVE
                     SMHVSGGCVTLDSALPVPRRHRAPVPVPPDEVRRLAAAETVAVIVDKD"
     gene            complement(1025..1831)
                     /locus_tag="VH419_00785"
     CDS             complement(1025..1831)
                     /locus_tag="VH419_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012920522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY7042180.1"
                     /translation="MARFLVVVVVVASVLVATLWLLQRRLIYFPDTGTVPPAATVIPG
                     ARDVTLRPSDGLELGAWFVPADEAGHGLTVLVANGNAGSRTDRAPLAEALADRGFGVL
                     LFDYRGYGGNPGSPSEEALARDVRAAYDFLVSSQGIEPDRLIYFGESLGAAVVTELAT
                     EYPPRAMVLRSPFVDLAAVGQVHYPFLPVRLLLRDEFPVADRIASVETPVAVVYGSKD
                     SVVPPRQSREVAAAARNAVVVEVDADHNDARLTYGDEVIDAVQRLAEQVD"
     gene            1938..>2241
                     /locus_tag="VH419_00790"
     CDS             1938..>2241
                     /locus_tag="VH419_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042181.1"
                     /translation="MSDDFLGVLQGKDDQDNSVRVSIDANHGMLKIGGAGRGGDVALT
                     DDAGTATIFIDAGGSEGESGGGPVHLSTDALLRAGATTVITGNGEIDLGGHRRTGWI"
BASE COUNT          387 a          770 c          772 g          312 t
ORIGIN      
        1 cgtccttgtc gacgatcacc gccacggtct cggcggccgc gagacgtcga acctcgtcag
       61 gcggcaccgg aaccggcgcg cggtggcgcc gcggtacggg cagggcgctg tcgagagtca
      121 cgcaaccgcc ggagacgtgc atggactcga ccgtcggcag gctgcgagag aagttcgcaa
      181 gtcttcggcc gaacgtaggt gctccctcga gcacggcacc gatccgggcg aggccacgac
      241 tgttgccggt cgccaccgcg tgcagcccag cgatcgggag aacacgctga aacggtgcaa
      301 ggcgggtgcg gttgaccagt gatccgttcg gcgtgatgtc aggcgtgaag ccgctgcgcg
      361 cgagcgagtc ggtcaccacc gaccagtcga gacctggtcg gaggccgaac acccgaccgc
      421 gtcccgtcga gcacagcatc gaccgcaggc aaccggtctc gacggtggcg aagtcgacct
      481 cccagtcgac gtcgtcgccg gtccagccga agtcgcgcga catcgtgcgc tcgaacgcgt
      541 tcagtgccga tccgttgaac atggatccgt ccgcctcggc gcgccgccag aaggcgacgt
      601 gctcagcgtg cgctgagttg ctggtcaacg atgcgaaccc cagccgctcc ttgaccgaac
      661 gtacgtcggt gaaggagatc tcagcggcgt tctccgggac cacggcgagg gcggcgaaca
      721 cccgcggata gttcagcagc gccgacaggt tcggcgcccc accggtgtcg gcgatggttc
      781 gagtggctgg gacaccgctg gcgcctgctg tcgcagtcgg cggcgactgg tccggctgcg
      841 gttgagggtg caccccggtg tcgcggtgac ccgtgcagcc cgcgaggagc agtacgagaa
      901 ccgccgcggc cgcgatcgcg gttcgccgcc ggacgtcgaa cggcgtacga cgtcgccggg
      961 ctgcagcggg catgcgtcga gtctctcacc ggcacaccag gtcgttgagc gccgtcgcca
     1021 ccgctcagtc cacctgctcg gcaagccgct ggaccgcgtc gatcacctcg tcgccgtacg
     1081 tcagacgcgc atcgttgtgg tccgcatcga cctcgacgac gacggcgttc cgcgcggccg
     1141 ctgccacctc acgactttgc cggggaggta cgaccgagtc cttgctcccg tagaccaccg
     1201 cgacgggagt ctcgacggag gcgatgcggt cggcgaccgg gaactcgtcg cggagcaaca
     1261 gccggaccgg gaggaacgga tagtggacct gtcccacggc cgccaggtcg acgaacggcg
     1321 accgcaggac catcgccctc ggcgggtact ccgtcgcgag ctcggtcacg acggcggcac
     1381 ccaggctctc cccgaaatag atcagccggt caggctcgat gccctgggac gagaccagga
     1441 agtcgtacgc cgcccgcacg tctcgcgcca acgcctcctc gctcggactt ccggggttcc
     1501 cgccgtaccc gcgatagtcg aacagcagaa cgccgaaacc gcgatcggcc aacgcttcag
     1561 ccaagggagc ccgatcggtc ctgctgcctg cgttgccgtt cgccacgagc actgtcagtc
     1621 cgtgccctgc ttcgtcggcc ggtacgaacc aggcgcccag ctcgaggccg tcgctgggtc
     1681 gaagggtgac gtcccgcgcg ccggggatca ccgtcgctgc cggtgggaca gtcccggtgt
     1741 cggggaagta gatcagccgc cgctgcagca accagagggt cgcgaccagc acgctggcga
     1801 cgacgaccac gaccacgaga aacctggcca tccgcccagt ctgcccgcac acggcttcga
     1861 ggagggtcgg acgaggtctg cgtcgctgta cgccgggagt acggtcgcgg caagaccgac
     1921 ccaacgagga gccagccatg tctgacgact ttctcggcgt actgcaaggc aaggacgacc
     1981 aggacaacag tgtccgtgtc agcatcgacg cgaatcacgg catgctcaag atcggtggag
     2041 caggtcgtgg cggtgacgtg gcgctcaccg acgacgccgg gaccgccacg atcttcatcg
     2101 acgcgggggg atccgaggga gagtccggcg gcggaccggt gcacctgtcg accgatgcac
     2161 tgctgcgggc aggagccacc acggtcatca ccggcaacgg cgagatcgac ctcggcggcc
     2221 accgcaggac cggttggatc a
//
LOCUS       DATBFZ010000026         2140 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_1983940, whole genome shotgun sequence.
ACCESSION   DATBFZ010000026 DATBFZ010000000
VERSION     DATBFZ010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1983940"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            166..726
                     /locus_tag="VH419_00795"
     CDS             166..726
                     /locus_tag="VH419_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042182.1"
                     /translation="MSVTITPETFTLVNFDAAEIKKVVDGLIPAIGLPADLDVTIEVD
                     ESTPLGRAIVTSYDDPVLINVESGALEDPKRPRRVSPTGAADVLGRLLFRVRDRRSDG
                     FGDPPPDEELTMPQSVAWDVYCIGRLVRLGFRHYDNRQRRLYHFRNRHGFTDAADEAF
                     ETLWTSDDLTWSDIVALSDKAAAAAA"
     gene            complement(804..1118)
                     /locus_tag="VH419_00800"
     CDS             complement(804..1118)
                     /locus_tag="VH419_00800"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HEY7042183.1"
                     /translation="MGTDMELTMVTMVFDSSDADALQSILAKYVVLSRGHPGCRNIDL
                     CASATTPGRFVIVQKWESPEAQQAHFDSPDMVEMASACTGLLAHPPAIDLLETISAHD
                     LI"
     gene            1412..>2140
                     /locus_tag="VH419_00805"
     CDS             1412..>2140
                     /locus_tag="VH419_00805"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY7042184.1"
                     /translation="MPKERVERDEEDLVRLYLTDIGQYPLLTKDDEVRLAQAIEGGNE
                     ARLHLERAGRDLTPARKRELRRQAREGEDAERTFVQSNLRLVVSIAKKYQASGLPLLD
                     LIQEGNLGLMHAVEKFDWRKGFKFSTYATWWIRQAITRGIANTGRTIRLPVHAGDTLA
                     RLQKARARLELKLGRPATLSELAAEVEMPEDKVTEALRFAAEPLSLSEPLREDGDAEL
                     GDIVEDRSVESPFEVAATALLPAEI"
BASE COUNT          382 a          718 c          690 g          350 t
ORIGIN      
        1 cggcttccgc ggcgaggacg acgacagccc catcaagctc gctcgtcgct tccgcgaact
       61 cgctgggttc gacagcgacg agatggacga cctcgcagag atctctcgcg aggccaaggc
      121 agatgcggag gcgcagcgcg agcggcttgc cgcacagtcg tccgcatgag cgtcacgatc
      181 acgcctgaga cgtttaccct cgtcaatttc gacgcggccg agatcaagaa ggtggtcgac
      241 gggctgatcc ccgcgatcgg gctccccgca gacctcgacg tcaccatcga agtcgacgag
      301 tcgacgccgc tcggtcgcgc catcgtcacc tcgtacgacg atcccgtgtt gatcaacgtc
      361 gagagtggcg cgctcgagga tccgaagcgt ccccgacggg tgtcgccgac cggggccgcg
      421 gacgttctgg gacggctcct gttccgcgtc cgcgaccggc gctccgacgg gttcggtgat
      481 ccgccgccgg acgaggagtt gaccatgccc cagtcggtgg cgtgggacgt ctactgcatc
      541 ggccgtctcg tccgcctcgg gttccggcac tacgacaacc gccagcgccg gctgtaccac
      601 ttccgcaatc gccacggctt caccgacgcg gccgacgagg cattcgagac tctgtggacg
      661 tccgacgacc tgacctggtc cgacatcgtc gccctctcgg acaaagccgc cgccgcagcc
      721 gcctagcccc ggtccccctt tccgttctcc gggtcgattg cggggctatg gccccacaat
      781 cgcaccaaca ttcgggagtg gggtcagatg aggtcgtggg cggagatggt ctcgaggagg
      841 tcgatggcgg gtggatgggc gagcaggccg gtgcaggcgg aggccatttc aaccatgtcc
      901 ggggagtcga agtgggcctg ctgagcttcg ggtgactccc acttctggac gatgacgaac
      961 cttccaggcg tggtggctga tgcgcagagg tcgatgttcc ggcacccagg gtggccgcgg
     1021 ctgagtacca cgtatttggc aaggatcgac tgcaacgcat cggcatccga cgagtcgaag
     1081 accatcgtga ccatggtgag ttccatgtcc gtccccatcg ctacgcaccg tagagcaggg
     1141 aacatcgccc cggttcccga agtccttgac cgtgcctgcc aaaaagtaca agttggacgt
     1201 caagggaccc caacctgcgt ccgaagaaca cgcagcgtga acatgaccat cgaaaaccaa
     1261 ggagcgggtt ggcactcttg acctggccag tatcgtaagg accacatact cgggccgtgc
     1321 gacggccgag tcgcttggcg ggctgaccac tccccgtcgt aagcgttccg ccgtacctcc
     1381 caaagttgga tttcgagcag agaggcctct cttgcccaaa gagcgcgtcg agcgcgacga
     1441 ggaagatctc gtccgtctgt acctcaccga tatcgggcag tacccgctgc tcaccaagga
     1501 cgacgaggtt cgtctggccc aggcaatcga aggtggcaac gaggcccgtc tccatctcga
     1561 gcgagccggc cgtgacctca ctcctgcccg caagcgcgag ctgcgccgtc aagcccgcga
     1621 gggtgaggac gccgagcgga cgttcgtgca gtcgaacctc cgcctggtcg tgtcgatcgc
     1681 caagaagtac caagcgtccg gtctgccgct gctcgacctc atccaggagg gcaacctcgg
     1741 cctgatgcat gccgtcgaga agttcgactg gcgcaagggc ttcaagttct cgacgtatgc
     1801 cacctggtgg atccgccagg ccatcacccg aggcatcgcc aacaccggtc gcacgatccg
     1861 cctgcccgtg cacgcgggcg acacgctcgc ccggctgcag aaggcgcggg cgcgcctcga
     1921 gctgaagctc ggccgtcccg cgacgctcag cgagctcgca gccgaagtgg agatgcccga
     1981 ggacaaggtc accgaggcgc tgcgtttcgc ggccgagccg ttgtcgctct ccgagccgct
     2041 tcgcgaagac ggcgacgccg agctcggcga catcgtcgag gaccgatcgg tcgagtcgcc
     2101 gttcgaggtc gcggcgaccg cgctcttgcc ggccgagatc
//
LOCUS       DATBFZ010000027         5757 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_4930796, whole genome shotgun sequence.
ACCESSION   DATBFZ010000027 DATBFZ010000000
VERSION     DATBFZ010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 5757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5757
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4930796"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2033)
                     /locus_tag="VH419_00810"
     CDS             complement(<1..2033)
                     /locus_tag="VH419_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042185.1"
                     /translation="MTQALESTPIRGQKDALRVAAGVGSGNFMFRVPVADYPGRAESP
                     LKLSLVYNSRVWQKVPYLPDQDRIVFDIDDDWPAPGWCFSLERLISSKSVPILVGPDG
                     TRHPAVVREEQVGPNGGKLVGQRTTDGSLITFYRERGSGVNSGYALYPDGARVVFDAR
                     SEDGGTLYPTRRIDRHGNVTRIDYRGGTGPNIERMVDPTGRTLTFQYDLTGQQLIAVK
                     GPGCYLAPDIVLLRLHYQWLNLDTSWSPGLDVDAPAQAVVIDGVMSPPSGQGYWFGDP
                     GDLSGYGMVRNVRKCVGMEWQPDLTDPPNPQGKMTKGIQIHLRSYNYPAASGVVLSDS
                     PTYTQMDEGYVGGITERTTFSVKPFSVSGTESTITYGDGSSTVQRDLPAADTSGLLSN
                     LSTFDPLHKLIQSVDTNWQPGADGAPFLTHVTTTSPISSWKVEYDPGPNNQVREERRY
                     RPGGGLDTRTVIEYEAAPVYTDRHILNLPKRIHVSDFGGTAISLTTYAYDTVALHPVP
                     GITGVASSYDPGSDIYDSRTAFRGCVTVERRYPIPRTGTAGIAVEVANEYDRAGNLVQ
                     QDLGPQRQTWVYDQSTEYALATRLIVGSKDPDSPARITNEAAYNPAGLMSWSRGVDGT
                     LTDYGYTMDGFRPMSLVHLEQPFYSVTILYDDLERRENLLVRDENGDLAINEVT"
     gene            complement(2524..2597)
                     /locus_tag="VH419_00815"
     tRNA            complement(2524..2597)
                     /locus_tag="VH419_00815"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2561..2563),aa:Ile,seq:cat)
     gene            complement(2660..3424)
                     /locus_tag="VH419_00820"
     CDS             complement(2660..3424)
                     /locus_tag="VH419_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042186.1"
                     /translation="MESGHVDGFLPSTSGLKFTNHFPSGIPIVSIELPGIGRSIPIGD
                     ASNGVCGGMVYAVLDLFTADPRLRVPEQTSPPDGDNPLTRYLTKRLVDSFALERGLGS
                     NVARYMHLMSTPDHDTYIVDGVASVIAEYEWPKIKRDIDAGRPSPLGLVAGKRVLPTD
                     FSGKVKQLGHCHQVLAYAYDVDDDRLTLHVYDPNDPGNDTSTVEMSLRDPSEDTPILT
                     QRISSRISGHGSFRAFFRHDHYTRVTPPDGISPGSL"
     gene            3622..4236
                     /locus_tag="VH419_00825"
     CDS             3622..4236
                     /locus_tag="VH419_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020385568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEY7042187.1"
                     /translation="MKIALYGASGMIGSRIAVEALSRDHAVTGLTRSGSIDVPPGCMP
                     RRGDAADADDVARIASEHDVVISAIGPSRTGVRHEVFLHALATLAEQVARRRLIVVGG
                     SGSLYVAPGLRLMDSPGFPPAAKPEQLTQATALELLKDTGALADWVYVSPAPFIAPGE
                     RRGHYRRGRDMVLGDQISAEDFALAIVDEVEQPKFHRSRFTVAW"
     gene            4255..5052
                     /locus_tag="VH419_00830"
     CDS             4255..5052
                     /locus_tag="VH419_00830"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY7042188.1"
                     /translation="MDVVSLGFRTDLALLRLGGSLIEDRGDHLVVTSPQNPTFWWGNF
                     ILLDHVPPPEAAGEWVDVFRREFPDQDHLAIGIDGRTRTREDAAGFAALGLKVETSSV
                     MTAQSVHEPPHPNMRAEYRRLESDQDWADHVELRVALEERYDKDSYRVFVTDKARSSR
                     AIVESGNGAWFGAFIDGRLLSTMGLVRADADVARFQNVETHPEARGQGLAGTLVEHVS
                     RYGFDELGATTLVMVADPDYVAIRVYRAVGFTSSETQLQIERTRGTD"
     gene            complement(5089..>5757)
                     /locus_tag="VH419_00835"
     CDS             complement(5089..>5757)
                     /locus_tag="VH419_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042189.1"
                     /translation="DGLLRSHVQYHADAATYIYAGSQPSLMRRLFTDRERPLYGQAEP
                     LELIPLPMDDVLVELAERFASLDLDPGEALAPLVVAAAGHPQRTMLLAHLLHRELELR
                     STGDSMIGSADLDGLALADAIVDLALDQTREAHQAIWDSLSSGKKAVLAALADGIRPT
                     GSRATERAGLSRATLQSALRELAREGQHVSRVKLGPESSPDWRFIDPLFARWVSLRGQ
                     RANT"
BASE COUNT          950 a         1847 c         1948 g         1012 t
ORIGIN      
        1 gtcacctcgt tgatcgccag gtccccgttc tcgtcacgta ccagaaggtt ctctcgacgt
       61 tccaggtcgt cgtagaggat cgtgaccgag tagaacggct gttcgaggtg caccaggctc
      121 attggacgga agccatccat cgtgtagccg tagtcagtga gcgtgccatc aacgcctctg
      181 ctccacgaca tcagcccagc cgggttgtag gcggcttcgt tcgtgattct ggctggtgag
      241 tcggggtcct ttgagccgac gatcagcctg gtggccagcg cgtattcggt gctctggtca
      301 tacacccagg tttggcgctg gggaccgagg tcctgctgca ccaggttgcc tgcccggtcg
      361 tactcgtttg caacctccac agcaatgcca gcagtcccgg tgcgcgggat cgggtaccgg
      421 cgctccacgg tgacacatcc tcggaaggcc gtcctgctgt catagatgtc gctgcccggg
      481 tcgtagctgg acgcgacgcc ggtgatgcca ggcactgggt gcagggcgac cgtgtcgtag
      541 gcgtaggtcg tcagcgaaat ggcagttcca ccgaagtcgg acacgtggat gcgcttcggc
      601 aagttcagga tgtgtcgatc ggtgtagacg ggggcggcct catactcgat gacagtccgc
      661 gtgtcgagac cgccgcctgg ccggtaccgg cgctcctcac gcacctggtt gttcggtcct
      721 gggtcgtatt ccaccttcca gcttgagatc gggcttgtgg tcgtgacgtg agtcaggaat
      781 ggcgcaccgt cggcgccagg ttgccagttg gtgtcgacag actggatgag cttgtgaaga
      841 ggatcgaagg tcgacaagtt cgacaacagc ccggaggtgt ccgcggcggg caggtctcgc
      901 tggacggtgg agctgccatc accgtaggtg atggtgcttt cggtgccgct gacgctgaac
      961 ggcttgaccg agaacgtggt gcgctcggtg atgccgccga cgtacccctc gtccatctgt
     1021 gtgtatgtcg gcgaatcact gagaacaact cccgatgcgg cgggatagtt gtacgaacgc
     1081 aagtggatct gaatcccctt ggtcatcttg ccctgcgggt tcggtggatc ggtcaggtcg
     1141 ggctgccact ccatgccgac gcacttgcga acgttgcgca ccatgccgta gccgctgagg
     1201 tcgcccggat cgccgaacca gtagccttgc ccgctcggcg gactcatgac gccgtcgatg
     1261 accaccgctt gcgccggggc gtccacatcg aggccgggac tccacgacgt gtccagattc
     1321 agccactggt agtgcagacg aaggagaacg atgtccgggg cgagatagca gcccggtccc
     1381 ttgacggcga tgagctgctg accggtcagg tcgtactgga acgtcagcgt ccgtccggtt
     1441 ggatcgacca tgcgctcgat gtttgggcct gtgccgccgc ggtagtcgat gcgggtcacg
     1501 ttgccgtgtc ggtcgatgcg tctggtcggg tacagcgtcc cgccgtcctc ggaacgagcg
     1561 tcgaaaacca cccgggcacc gtccggatac agcgcgtatc ccgagttcac accgctgccg
     1621 cgttcgcggt agaaggtgat cagggatccg tccgttgtcc gctgaccaac gagtttccca
     1681 ccgttgggtc cgacctgttc ttcacgtacg acggcgggat gacgggtgcc gtccggtccg
     1741 acgaggatgg gaaccgactt gctcgaaatc agccgctcga gcgaaaagca ccacccaggg
     1801 gccggccagt catcgtcaat gtcgaagacg atccgatcct ggtcgggcag atagggaacc
     1861 ttctgccaaa ccctcgagtt gtagaccagt gacagcttca gagggctctc cgcccgacct
     1921 gggtagtccg cgacaggcac cctgaacatg aaattgccgc tgccgacgcc tgctgccacc
     1981 ctcaacgcgt ccttctggcc gcgaatcgga gtggattcca gcgcttgcgt cattgccttg
     2041 gcgtagggaa ttcctccccg ccgtgatgac ggagcagtcg caacagcttg tgcggcttct
     2101 tcccagcccg ttctgcgcat cgccgacccc ctgacggacc cggcgagccg gatccacccg
     2161 cccgccggcg gtaacctccc ccgtccactc tcgcaggatg gagaacattg ctccacccac
     2221 atgtcgacga actcacggga ctgagggagc tatcccgcgg aaaccgagtc gttgggttga
     2281 cgtcaacatt cgttggcaac ggagcgatgc cacatttctg caaactgtcc aagccgtcgt
     2341 tctcagcact gcttccggtt ggcgggcgga ggctctcgtc gccgatggga gcaggtgaag
     2401 gaagcgatgg acgctgccct agccaccgtc caacgatggt tccctggcaa tctccccgcc
     2461 aagcgtcctg gtctgtgccc acctggtcgc tctccggaag gtaaagatcc agttcaagtg
     2521 gggtggggcg ggcggggatc gaacccgcga cctaaggatt atgagtcctc tgctctgacc
     2581 ggctgagcta ccgccccctg aacggcacag atcatgtcac gccgccgggc agcgggggcc
     2641 acgcagcggg cgaggccggt cagagcgagc cgggcgagat gccgtcgggt ggggtcacac
     2701 gcgtgtagtg gtcgtggcgg aagaacgccc ggaacgagcc gtgaccggag atccgggacg
     2761 agatccgttg ggtgagaatc ggtgtgtcct ctgacgggtc tcgcaacgac atctcgacgg
     2821 tcgacgtgtc gttgcccggg tcgtttgggt cgtacacatg cagcgtgagg cggtcgtcgt
     2881 cgacgtcgta cgcgtaggcg agcacctggt ggcagtggcc gagctgtttc accttgcccg
     2941 agaagtcggt cggtagcaca cgcttcccgg ccaccagccc gagtggagac gggcggcccg
     3001 cgtcgatgtc ccgcttgatc ttcggccact cgtactcggc gatgaccgac gcgaccccgt
     3061 cgacgatgta cgtgtcgtga tcgggtgtcg acatgaggtg catgtaccgg gcgacgttcg
     3121 aaccgagacc gcgctcgagg gcgaagctgt cgacgagacg cttcgtgagg tacctcgtca
     3181 gcgggttgtc accgtccggc ggcgacgtct gctcgggcac acgaagtcgc ggatcggcgg
     3241 tgaagaggtc gaggacggcg tacaccatgc ccccgcagac gccgttggag gcgtcaccga
     3301 tcgggatgga gcggccgatg ccgggcagct cgatgctcac gatcgggatc ccggagggga
     3361 agtggttggt gaacttcagg cccgacgtgc tcggcagaaa tccatcgacg tgtccgctct
     3421 ccatcagctc caccttcccg gaccatgcaa caagggccac gacgccggcc cgacggcgcg
     3481 cacgtcgcct cgttgcatgg tcgcccgact gtcaagactc gccatccccg caggagccgg
     3541 tcccgccgtc tcctgatacg caaggtccgc aacgcagtcg acctatgatg tgcgcacacc
     3601 gagccagtgc cgggagggct gttgaagatc gcgttgtacg gcgcctccgg catgatcgga
     3661 tcgcgcatcg ccgtcgaggc actgtctcgc gaccacgccg tcaccggact gacccgttcg
     3721 ggaagcatcg acgtgccgcc cggatgtatg ccgcgacgag gcgacgcagc cgatgccgac
     3781 gacgtggcgc ggatcgcgtc cgagcacgac gtggtgatct ctgccatcgg tcccagccgg
     3841 accggcgtac gccatgaggt cttcctgcac gcgctcgcca cgcttgccga gcaggtcgcc
     3901 aggcgtcgac tcatcgtcgt cggcggctcc ggcagcctgt acgtcgcgcc aggcctgcgt
     3961 ctgatggaca gcccaggctt cccgccggcc gccaagcccg aacagctgac ccaggcgacg
     4021 gcgctcgagc tgttgaagga caccggcgcg ctcgccgact gggtctacgt gtcgcccgct
     4081 ccgttcatcg cgcccggcga aaggcggggt cactaccgcc gcggccgcga catggtgctc
     4141 ggcgaccaga tcagtgccga ggacttcgcc ctcgccatcg tcgacgaggt cgagcagccg
     4201 aagttccacc gttcgcggtt caccgttgcc tggtgatggg agaggcttgg acccgtggac
     4261 gtcgtctcgc tgggctttcg gaccgacctc gccctgctcc ggctcggcgg gtcgctgatc
     4321 gaggaccgcg gtgaccatct cgtggtcacg tcgccacaga acccgacgtt ctggtggggc
     4381 aacttcatcc tgctcgacca cgtgccgccg ccggaggctg ccggcgaatg ggtcgacgtc
     4441 ttccgacggg agttccccga tcaggatcac ctcgccatcg gcatcgacgg gaggacgagg
     4501 acgcgtgaag acgcggcggg ctttgccgct ctggggctca aggtcgagac gtcatcggtg
     4561 atgaccgcgc agtccgtcca cgagccacca cacccgaaca tgcgtgcgga gtaccgccgg
     4621 ctggagtccg accaggactg ggccgaccac gtcgagctcc gggtggcgct cgaggagcga
     4681 tacgacaagg actcgtaccg cgtcttcgtg acggacaagg cgcgcagctc gcgcgcgatc
     4741 gtcgagtccg ggaacggcgc gtggttcggg gcgttcatcg acgggcggtt gctctcgacc
     4801 atgggactgg tccgggccga cgcagacgtt gcccggttcc agaacgtcga gacccatccg
     4861 gaagcccgcg gccaggggct ggccgggacg ctggtcgagc acgtgagtcg gtacgggttc
     4921 gacgagctcg gcgccaccac gttggtgatg gtcgcggatc cggactacgt ggcgatcagg
     4981 gtctatcgcg cggtcgggtt cacgtcatct gagacgcagc tgcagatcga acggacgcga
     5041 gggacggact gagcgcgtcg atcggcgcgc ccggacgacg tcaggccctc acgtgttggc
     5101 gcgttggccg cgcagcgaca cccaccgagc gaacagcggg tcgatgaacc gccagtccgg
     5161 gctcgactcc gggcccagct tcacccggct gacatgctgc ccttcgcgcg cgagctcgcg
     5221 cagcgccgac tgcaacgtgg cgcggctgag accggcgcgt tcggtggcgc gtgaaccggt
     5281 cggccggatg ccgtcggcca gggcggcgag gaccgccttc ttgcccgagc tcaagctgtc
     5341 ccagatcgcc tgatgcgcct ctcgcgtctg gtcgagggcc aggtcgacga tcgcgtcggc
     5401 aagcgccagc ccgtccaggt cggcggatcc gatcatcgag tcaccggtgc tgcgcagttc
     5461 gagctcccga tgcagcaggt gggcgaggag catcgtgcgc tggggatgac ccgctgccgc
     5521 gacgacgagc ggcgccagcg cctcgccggg atccagatcg agtgacgcga accgctcggc
     5581 gagctcgacg aggacgtcgt ccatcgggag cgggatcagt tccagtggct ctgcctgtcc
     5641 gtacagaggt cgttcgcggt cggtgaagag gcggcgcatc agcgacggct gcgaacccgc
     5701 atagatgtag gtcgccgcgt ccgcgtggta ctgcacgtgc gagcgcaaca gcccgtc
//
LOCUS       DATBFZ010000028         7236 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_1987048, whole genome shotgun sequence.
ACCESSION   DATBFZ010000028 DATBFZ010000000
VERSION     DATBFZ010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 7236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7236
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1987048"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..892
                     /locus_tag="VH419_00840"
     CDS             <1..892
                     /locus_tag="VH419_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042190.1"
                     /translation="KRGAAWAVGVAATGGLVLMVVDAADGASRLAKVNDLLDSYGHAS
                     SAAPAPAAAPAPVAVAPTTSAPTPSTTTTLVIPTTDAPKLEIPRTVRIDGENVSLAHV
                     IDLGLIGEEPEIWTAAQERTELAADYEKDGTLPCEGSDCSGLTVADYQQMWGTTDPQV
                     MLDKAAQYWADWTVGRHKEWIASLGYLDTTEGRQDYHEREARWQQEQAKYAEQDRKPV
                     LQQAAEDLNELQGDGSVPAYEPGDWAHRTMAGVDVKALCRSVDEKGYRLAKHQFEDEH
                     GGNGDDVIYAYATLVSEHCH"
     gene            967..1551
                     /locus_tag="VH419_00845"
     CDS             967..1551
                     /locus_tag="VH419_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042191.1"
                     /translation="MNFSDEPTVRPIGGDELVTVSYVLPARLATKFQREAGAVLDRLH
                     RIDAEPDFDLAIWQSSGFAPPYNSNHRGDESAWTFPPITWDDRKAVAWAVDNWTDTGR
                     SLVAGLLLAPAAGVHTFDLAEAVGYTGGLPSAFRAIVGRLRAIERAPFWRGDPDTLRH
                     ERGQLLAPDRGADVVRRILEIRHPAVFATNGRTR"
     gene            1605..2123
                     /locus_tag="VH419_00850"
     CDS             1605..2123
                     /locus_tag="VH419_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042192.1"
                     /translation="MQLESTGPDVWLFRCVRGPQHADRDHVWSPTPLVVDLDRRTGLG
                     EELGVYDDLLRCVEPGERVEYGVVEHRFAAVSPDAYRDLLVRYGHTSLAPTKYSTSSF
                     LSHALAQLGREGLLVVTSVDATGYWRYLRAVPAFARPETPPDIETTSWAGYASHHGID
                     PATWPASAALRR"
     gene            complement(2137..2529)
                     /locus_tag="VH419_00855"
     CDS             complement(2137..2529)
                     /locus_tag="VH419_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042193.1"
                     /translation="MRDVYESDRYLDVLEALWARGAGTWYSYGDLAEVIQLHFAVQQD
                     GVTISNVLKRVAQRHGTPNRARKVDGTAGHNFDPLLQRLWDERMQSEGLPPWDEQWPE
                     WRRVRSHALIDMLSDILPTQIIYEPVTS"
     gene            complement(2641..3009)
                     /locus_tag="VH419_00860"
     CDS             complement(2641..3009)
                     /locus_tag="VH419_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042194.1"
                     /translation="MITSPDEYLATVPEVGRPWLREFWDYVQNTYPDLEVTMFRQRPM
                     YKFGHSYQHGYVMFTAAKTHFAVQAIDFDLVARARSTIPGALGGKGNVAVKYTATEAK
                     PALKAFVDAVMSRHDVGRRT"
     gene            complement(3122..4099)
                     /locus_tag="VH419_00865"
     CDS             complement(3122..4099)
                     /locus_tag="VH419_00865"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEY7042195.1"
                     /translation="MITYVDTSVIVKLIIDEPESDHASMIWTSAASLAACQLAHVEAR
                     AALASAHRAGRVTRAELDNAEANLVELWSQLYVVDVDESLVRHAGDLARLHALRGYDA
                     VHLGAALFVGARVMAVSDGRLARAARECGIAVAVPSELALADVVHLTGPVVEIMHRWL
                     EAIVDRGDLAAAWPLTEPDFRLALVQSWLWEGFAPQVPNVEVAAALLAEPDQPAHPLW
                     DEFAAWRIGRWKAVLPDYVIDPSRRGTVSSPYISGVDLEAVMISDNSTGARILGGRTV
                     EVQRFSVRHTVDGVLVSGVGAALPVPGWPPTEIPLPPLPADPQLGERRS"
     gene            complement(4599..5096)
                     /locus_tag="VH419_00870"
     CDS             complement(4599..5096)
                     /locus_tag="VH419_00870"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEY7042196.1"
                     /translation="MTVWHVDKNPEAKTMVITAEFKASIATVWRLWADPRLLERWWGP
                     AGFPATFEHHDLTPGGTITYFMSGPEGGDRFDGTWNVIEVDAPTRLVVEDAIVEEDGT
                     PSDGNSMTRLEIDIEAAGDTTRMILTTHFDSLEGMEQAIAMGVVEGMKACMSQIDTLL
                     ADIAA"
     gene            complement(5093..5446)
                     /locus_tag="VH419_00875"
     CDS             complement(5093..5446)
                     /locus_tag="VH419_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017932813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEY7042197.1"
                     /translation="MVVDTVERERDTDRLFFALSDATRRDIVTQTLDRERSVSELGRL
                     YPISLTAVQKHVEVLVDAGLVSKHRSGREQLVRGEVDRLRRAHDLLDDLETLWRARLE
                     RFQDVLNETTGKENQ"
     gene            complement(5593..5877)
                     /locus_tag="VH419_00880"
     CDS             complement(5593..5877)
                     /locus_tag="VH419_00880"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HEY7042198.1"
                     /translation="MTKRTSGHTTSGKPIDDQLIDALANEAEAGYDVDEILARRGKRG
                     RPSLGNAPSTVESVRLDPDLKERLARRAEAEGVPVSEVIREALRQHLEAS"
     gene            complement(6210..6872)
                     /locus_tag="VH419_00885"
     CDS             complement(6210..6872)
                     /locus_tag="VH419_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020576438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEY7042199.1"
                     /translation="MTQLVRVQNFMLSTDGFGAGEGQSLEAPFGHADRPDVSVPADLA
                     SWAFATASWPGRTEPGGSRGLDDYFTRDFARNIGAEIMGRNKFGPQRGPWENLDWEGW
                     WGDNPPFHTPVFVLTHHHRPSFTLTDTTFHFVDATPEDAMRQAKEAAEGKDVRIGGGV
                     ATVRQFLEADLIDTIHVAVAPIEIGRGERLWRSHEDLLDRFHLESVPSPSGVTHLLFW
                     RR"
     gene            complement(6874..>7236)
                     /locus_tag="VH419_00890"
     CDS             complement(6874..>7236)
                     /locus_tag="VH419_00890"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEY7042200.1"
                     /translation="CQWFGPDGFTCTVRAMDVRPGGRACFDMTSGDGTVFSNRFDYLE
                     IVPSERLVLDHGADTDDDPKRFRVTITLDEQADGKTVLTLRQLHPTVELRDTKIGFGA
                     VELGLQTMQKLARYLGAA"
BASE COUNT         1182 a         2323 c         2436 g         1295 t
ORIGIN      
        1 gaagcggggc gccgcctggg cggtcggcgt cgcggcaacg ggcggcctcg tgctcatggt
       61 ggtcgatgcg gccgatggcg ccagccgcct ggccaaggtc aacgaccttc tcgacagcta
      121 tggtcacgcc tcgtcggccg ctccggcgcc ggccgcggca cccgcccctg tcgccgtcgc
      181 gccgacgacg tccgcgccga caccgtcgac gacgacgacg ctcgtaatcc cgacgaccga
      241 tgctccgaag ctcgagatcc ccaggaccgt acgcatcgac ggtgaaaacg tgtcgctcgc
      301 gcacgtcatc gacctgggcc tcatcggcga agagcctgag atctggacgg cggcgcagga
      361 gcggaccgag ctcgctgcgg actacgagaa ggatgggacg ctgccctgtg aaggcagcga
      421 ctgttccggg ttgacggtgg ccgactatca acagatgtgg gggacgacgg acccgcaggt
      481 gatgttggac aaggcggcgc agtactgggc cgactggacg gttgggcggc acaaggagtg
      541 gatcgcgagc ctcggctatc tcgacacgac cgagggacgc caggactacc acgagcgtga
      601 ggcccggtgg cagcaggagc aggcgaagta cgccgaacag gatcgcaagc cggtgctgca
      661 gcaagccgcg gaggatctca acgagttgca gggcgacggg tcggttcccg cctacgagcc
      721 cggcgattgg gcgcacagga ccatggccgg cgtcgatgtg aaggcgctgt gccgctccgt
      781 cgacgagaag ggctaccgcc tcgccaagca ccaattcgag gatgagcacg gtggcaacgg
      841 cgacgacgtc atctatgcgt acgccacgct cgtcagcgag cactgtcact gagattcatc
      901 atctcagttc acaatttctg cgatgctgac ttgacgacct cccgactgac atcttagcat
      961 cgcagaatga acttctccga tgaaccaacc gtgcggccga tcggcggcga cgagctggtc
     1021 accgtcagct acgtcctccc cgctcggctt gccaccaagt tccagcggga ggccggagct
     1081 gtgctcgacc gcctccaccg catcgacgcc gaacccgact tcgacctcgc gatctggcag
     1141 tcgtccggct tcgcgccgcc gtacaacagc aaccaccggg gcgacgagag cgcctggacc
     1201 tttccgccga tcacgtggga cgaccgcaag gcggtggcgt gggccgttga caactggacc
     1261 gatactggcc ggtctctggt cgccggtctc ctcctcgctc ccgcggcggg tgtccacacc
     1321 ttcgacctcg cagaggccgt cgggtacacg ggcggcctgc cgtccgcgtt cagagcgatc
     1381 gtgggtcgcc ttcgggcgat cgagcgagca ccgttctggc ggggcgatcc cgacacgctt
     1441 cgccacgaac gcggtcagct gctcgcaccc gatcgaggag ccgacgtcgt gcgtcgcatc
     1501 ctcgagatca gacatccggc cgtcttcgcg acgaacggca gaacccggtg actccgaggc
     1561 gtccaccccg gcaggtctgc gccctgtgcg gactcgatga cttcgtgcag ctcgaatcga
     1621 ctggccctga tgtgtggctg ttccgttgcg tacgaggacc gcaacacgcc gaccgagacc
     1681 atgtctggtc gccgacgcca ctggtcgtcg atctcgaccg tcggaccggt ctcggcgaag
     1741 agctcggtgt ctacgacgac ctcctgcgct gtgtcgagcc cggcgagcgg gtcgagtacg
     1801 gcgtcgtcga gcacagattc gcagccgttt caccggacgc ctaccgcgac ttgctggtcc
     1861 gctacggcca cacgtcgctg gcaccgacca agtactcgac gtcgtcgttc ctgagccatg
     1921 ccctcgcgca gctcggtcgg gaaggtctcc tcgttgtcac gagcgtcgac gccaccggct
     1981 attggaggta cctgcgcgcg gttccggcct tcgcgcggcc ggagacacca ccggacatcg
     2041 agacaacgtc gtgggccggg tacgcctccc accacggaat cgatcccgcg acctggccag
     2101 ccagtgcggc gctgcgccgt tagcgccgac agctcctcag gatgtcaccg gttcatagat
     2161 gatctgggtc gggaggatgt ccgagagcat gtcgatcagc gcgtggctcc gcacccgtcg
     2221 ccactcgggc cactgctcgt cccagggcgg tagaccctct gactgcattc gctcgtccca
     2281 gaggcgctgg agaagtgggt cgaagttgtg accggcggtg ccgtccacct tgcgtgctcg
     2341 gttgggggtg ccgtggcgct gggccacccg cttgaggacg ttggagatcg tgacgccatc
     2401 ctgctggacg gcgaagtgca gttggatgac ctcggcgaga tcgccgtagg agtaccaggt
     2461 gccggcgccg cgagcccaca acgcctccag gacgtcgagg taccggtcac tctcgtagac
     2521 gtcacgcatg accaacctcc cggccgatgg agccggcctc ccactggcaa accacggtga
     2581 tcatgagttc ctttcccgcc gcggaaaggt gactctactg gctggtcaag gccggttttg
     2641 ttaggtcctc cgacccacgt cgtggcgact catgacggcg tcgacgaagg ccttcagtgc
     2701 tggctttgcc tccgtcgccg tgtacttcac tgcgacgttg cccttgcctc cgagagcgcc
     2761 cggaatagtt gagcgggctc gtgcgaccag gtcgaagtcg atggcttgga cggcgaagtg
     2821 ggttttcgcc gccgtgaaca tgacgtaccc gtgctggtag ctgtgtccga acttgtacat
     2881 cggtctctga cggaacatgg tcacttcaag gtccgggtac gtgttctgga cgtagtccca
     2941 gaactcccgc agccacggtc gaccgacctc gggcacggtt gcgaggtatt catcgggact
     3001 ggtgatcacg ctgggagtgt cgcaccccgg cttcgggctc ttcctcacgc tcccggtcga
     3061 tcgaagggaa ggttcttcga cgacgatggt cacagacccg gtggtcgggg atgcttgggc
     3121 gctaggaccg acgttcgccg agttgcgggt cggctggcaa tggaggaagg gggatctcgg
     3181 tcggtggcca gcctgggaca gggagcgccg cgccgactcc tgagaccaag acgccgtcga
     3241 ccgtgtggcg aacgctgaaa cgttgtacct ccacggttcg tcctcccagg attcgagcgc
     3301 cggtgctgtt gtcgctgatc attaccgctt cgaggtcgac acccgagatg taaggagacg
     3361 acacagtccc tcgcctcgac gggtcgatga cgtagtccgg caacactgcc ttccaccgac
     3421 cgatccgcca cgccgcgaac tcgtcccaca gaggatgcgc gggttggtct ggctcggcta
     3481 aaagcgccgc cgcgacctcg acgttgggta cttgtggggc gaatccctcc cacagccagg
     3541 actgcacgag cgcaagacgg aagtcgggct cggtcagcgg ccaggctgcg gcgagatccc
     3601 cgcgatcaac gatcgcttcc agccagcggt gcatgatctc cacgacgggg cctgtcagat
     3661 ggaccacgtc ggccagggcg agctcgctcg ggacggcaac cgcgatgccg cactcgcgtg
     3721 cggcacgtgc gagacgcccg tcgctgaccg ccatcactcg cgctccgaca aagagcgcgg
     3781 caccgagatg aaccgcgtca tagccacgta aggcatgcaa tcgtgcgagg tcacccgcgt
     3841 gtcgaacgag ggactcgtcg acatccacga cgtagagctg agaccacaac tcaacgagat
     3901 ttgcttcggc gttgtcgagc tcagccctgg tgacccgacc cgcccgatgt gctgaggcaa
     3961 gtgcggcgcg agcttcgacg tgcgcgagtt ggcaggctgc gagcgacgcc gccgacgtcc
     4021 agatcatgct tgcatggtct gattccggct cgtcgatgat gagcttcaca atcaccgacg
     4081 tatctacata ggtgatcacc tacgttggtc gtccaccagg tcgctcacgg cacctctcgc
     4141 gacgacccgt cgtggggtgt ggcgttcagg cgaggtggga gggcgaacgc tcgactgctc
     4201 gacgagcgcc cgcagccgac ggtcctcgtc gccgactggc aggatgaccg caacgggaac
     4261 accgcggtcc gtaaccgtca gaacggtccc atgcgcgaca tcgtccaaat gacggctcaa
     4321 attcgtcttg aggtcccgca ctcccacatc tcgccgatcg tgcgatgtag tcacagccat
     4381 gcgcatattg tagtgctcgt cgagcaacgc tggtcgctcg cgccccaagg gtgccgcccg
     4441 ggccacccga ggcccgagcg ggattctggc ggaggtggcc cagtagcgac atgcgcagcc
     4501 atgtcgccac cgggctcacg tgcatgggtt gtctcggcgg tgtgggccgt cgtgagcccg
     4561 gtgctggcgg cgaagttcgt ccgctgagag gaatccccct aggcggcgat gtcggcgaga
     4621 agggtgtcga tctgggacat gcaggccttc atgccttcga cgacgcccat ggcgatcgct
     4681 tgctccatcc cttcgagcga gtcgaaatgc gtggtgagga tcatgcgggt cgtgtcgcct
     4741 gccgcttcga tgtcgatctc caggcgagtc atcgagttgc catcgctggg cgttccgtcc
     4801 tcttcgacga tggcgtcttc gaccacgagc ctggtcgggg cgtcgacctc gatcacgttc
     4861 caggtgccgt cgaacctgtc gccgccctcg gggccggaca tgaagtaggt gatcgtgccg
     4921 cccggtgtga ggtcgtggtg ctcgaacgtg gccgggaacc cggccggacc ccaccaccgc
     4981 tcgaggaggc gcgggtcggc ccacagccgc cacacggtgg cgatggacgc cttgaactcc
     5041 gccgtgatga ccatggtctt ggcctctggg ttcttgtcga catgccaaac ggtcattggt
     5101 tttcctttcc cgtggtttcg ttgaggacgt cttggaagcg ttcgaggcgg gctcgccaca
     5161 gcgtttcgag gtcatcgaga aggtcgtggg cgcggcgcag tcggtcgacc tcgccccgca
     5221 cgagttgttc ccgtccgctc cggtgcttgg agaccagacc ggcgtcgacg aggacctcga
     5281 cgtgcttctg gacggcggtg aggctgatcg gatacagccg gccgagctcg ctgacagagc
     5341 gctcacggtc gagcgtctgg gtcacgatgt cgcgtcgtgt tgcatcgctg agcgcgaaga
     5401 acaaccggtc ggtgtctctt tcgcgctcga cagtatctac aaccatttgg ttgtaggtta
     5461 cgcctcccgc cgacgatgtc aagaggccag cgctcgctgc gtccatcggg gtgcctggag
     5521 gcgtggcgta ggcgttcctg gaccgatgct gcgccgaagg acgctcacag ccggcgctct
     5581 gggacggttt gttcagctcg cttcgaggtg ttgtcgaagt gcctcgcgga tgacttcgga
     5641 gacggggact ccctcagcct ctgctcgccg tgcgaggcgt tccttgaggt ctgggtcaag
     5701 ccggaccgac tcgaccgtcg atggagcgtt gccgaggctg ggacggcctc gcttgcctcg
     5761 gcgggcgagg atctcgtcga cgtcatagcc agcttcggct tcgttggcca gcgcgtcgat
     5821 gagctggtcg tcgatgggct tgcctgatgt ggtgtggccg gaggttcgct tggtcatggc
     5881 tcgaacagct tccggtaaac gggccgcagg ggcatggcgt ggatgatcac ttcatcgcct
     5941 tcgatcgagt ggaggatgtc gtcgtccgcg attccgtggc ggcgggctga cgagcgaatc
     6001 tctacacacg attattgtac tacgaaatca cgagatgtcc gcccccggcg acgaaggcgg
     6061 cgtgccggtc gaaggtatgt cgggcggtgg tcgcccgagg tcgtgtgggg atccgcgcgt
     6121 cgatcggcca aatgaacgtc gcgcaatgga gcgccggcgt cgagcgccgg catgggctga
     6181 ccacggacgg ggcccgtcgt cggccgtggt taccgccgcc agaagaggag gtgcgtgacg
     6241 ccgctggggc tgggcacgga ctcgaggtgg aaccggtcga gcaggtcctc gtgggacctc
     6301 cagagtcgct cccctcggcc gatttcgatc ggcgcgacgg cgacgtggat ggtgtcgatg
     6361 agatccgcct cgaggaactg ccgaacagtg gcgacgccac ctccaatccg tacgtctttg
     6421 ccctcagcgg cctccttggc ttgccgcatc gcgtcctcgg gcgtcgcgtc gacgaagtgg
     6481 aacgtggtgt cggtcagcgt gaagctgggt cggtgatggt gggtgaggac gaacacgggc
     6541 gtgtggaagg gcgggttgtc tccccaccac ccttcccagt cgaggttctc ccacgggcca
     6601 cgctgcggcc cgaacttgtt gcggcccatg atctcggcac cgatgttccg cgcaaagtcg
     6661 cgagtgaagt agtcgtcgag ccctcgggat ccgccgggct cggttcggcc cggccagctc
     6721 gcagtggcga acgcccacga ggccaagtcg gccggaacgc tgacatctgg gcggtcggcg
     6781 tgtccgaagg gcgcctcgag gctctggccc tcgcccgcgc cgaagccgtc cgtcgacaac
     6841 atgaagttct gcactcgcac gagctgggtc atctcaggcg gctccgaggt agcgggcgag
     6901 cttctgcatc gtctgcagac cgagctcgac cgcgccgaag ccgatcttcg tgtcgcgcag
     6961 ctcgacggtc gggtggagct gccgcagcgt cagcaccgtc ttcccgtcgg cctgttcgtc
     7021 gagggtgatc gtcactcgga agcgcttcgg gtcatcgtcg gtgtcggcgc cgtggtccag
     7081 gaccagccgc tcgctcggaa cgatttcgag gtagtcgaag cgattgctga acacggtgcc
     7141 gtctccggag gtcatgtcga agcacgctcg gccgccgggg cgcacgtcca tcgctcggac
     7201 cgtgcaggtg aacccatcgg ggccgaacca ttggca
//
LOCUS       DATBFZ010000029        10169 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_2642945, whole genome shotgun sequence.
ACCESSION   DATBFZ010000029 DATBFZ010000000
VERSION     DATBFZ010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 10169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10169
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2642945"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(164..1525)
                     /gene="pafA"
                     /locus_tag="VH419_00895"
     CDS             complement(164..1525)
                     /gene="pafA"
                     /locus_tag="VH419_00895"
                     /EC_number="6.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017935380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA];
                     GO_process: GO:0070490 - protein pupylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Pup--protein ligase"
                     /protein_id="HEY7042201.1"
                     /translation="MDRRILGIENEYGVTCTFKGARRLSPDEVARYLFRRVVSWGRSS
                     NVFLRNGARLYLDVGSHPEYATPECDSVTDLVAHDKAGERVLEGLLVDAERRLREEGI
                     AGDVYLFKNNTDSAGNSYGCHENYLVGRHGEFARLADILIPFLVSRQIICGAGKVLQT
                     PRGAIYSVSQRAEHIWEGVSSATTRSRPIINTRDEPHADAERFRRLHVIVGDSNMSET
                     TTMLKVATTDLVLRMIEAGVVMRDMTMENPIRAIREISHDMTGQRRVRLANGRDASAL
                     DIQNEYLAKARDFVDRRELHTPTVDAALDLWERGLKAVESDDLSLVEREIDWVIKYRL
                     IERYRSQHGLGLSSARVAQLDLAYHDIHRRRGLYYLLERRGAVARVTSDLAIFEAKSV
                     PPQTTRARLRGEFIRRAQERRRDFTVDWVHLKLNDQAQRTVLCKDPFRSHDERVERLI
                     EGM"
     gene            complement(1670..2236)
                     /locus_tag="VH419_00900"
     CDS             complement(1670..2236)
                     /locus_tag="VH419_00900"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY7042202.1"
                     /translation="MRSATFLTSRMPDDARNWQVVGDDTVIHQGWIRVSRRTYRLPDG
                     RVAEWEMFGGGQTVGVLALTPDGRLLCVRQFRPGPDRVVLNIPGGFVDEGETPIEAAA
                     RELTEETGYTSDDMELVATARVASSTGQRHVVIARDCRPDGVQNLDAYEDCEPVVLDV
                     ADVREQLRNGGMTGVDIVYLGLDHAGLL"
     gene            complement(2199..2999)
                     /gene="prcA"
                     /locus_tag="VH419_00905"
     CDS             complement(2199..2999)
                     /gene="prcA"
                     /locus_tag="VH419_00905"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020384868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit alpha"
                     /protein_id="HEY7042203.1"
                     /translation="MSMPFYVSPEQLMKDRADFARKGIARGRSLVVLQYVDGLLFASE
                     NTSPSLHKVSEIYDRLAFAAVGRYNEFENLRIAGVRLADLRGYSYDRRDVTGRGLANA
                     YAQTLGTIFSSGGEKPYEVEIFVAEVGDTAAEDQIYRLTYDGSVADVQGYAVMGGAVE
                     SVNDYLSSRYAQGLDLAAALRLAVDALGHEGAAAANRQVNRDNLEVAILDRTRTQPRK
                     FRRLTGARLAALFDAAPPEEETSPESSTESPAEDPADAIGDLPDEPDA"
     gene            complement(3011..3838)
                     /gene="prcB"
                     /locus_tag="VH419_00910"
     CDS             complement(3011..3838)
                     /gene="prcB"
                     /locus_tag="VH419_00910"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit beta"
                     /protein_id="HEY7042204.1"
                     /translation="MSQPRLPGAYVTPGSSSFAEFLAAQAPELLPGRRTPVSGTMPET
                     PHATTLVAATFEGGVVMAGDRRATMGNIIAQRDIEKVFPADEYSCVGMAGAAGIGLEL
                     IRLFAVELEHYEKIEGTTLSLDGKANRLAALIRSNLGMAMQGLAVVPLFAGYDLHAGV
                     GRIFSYDATGGRYEEHAFHSVGSGSVFARGSLKKLYRDDLTARESVMATVQALYDAAD
                     DDSATGGPDLTRRIFPVVAMVTEDGFSRLGDSEIAGIADEVVAARMQRPDGPSAPLT"
     gene            complement(3868..4077)
                     /locus_tag="VH419_00915"
     CDS             complement(3868..4077)
                     /locus_tag="VH419_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012920843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like protein Pup"
                     /protein_id="HEY7042205.1"
                     /translation="MAREGGQQHRTTRKSSEDDAPPEAAPSEAAAERKEKLDDDVDAI
                     LDDIDEVLESNAEEFVRGFVQKGGQ"
     gene            complement(4157..5662)
                     /gene="dop"
                     /locus_tag="VH419_00920"
     CDS             complement(4157..5662)
                     /gene="dop"
                     /locus_tag="VH419_00920"
                     /EC_number="3.5.1.119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="depupylase/deamidase Dop"
                     /protein_id="HEY7042206.1"
                     /translation="MTVRRIMGTETEFGIHVLGTPSANPMLTSSQLVNAYASTSQRSR
                     RARWDFEEESPLRDARGFDLSRSAADPSQLTDEEIGLANLILTNGARLYVDHAHPEYS
                     TPECTNPRDIVLWDKAGELIIAAAAAAVDIPGQNPLVLYKNNTDNKGASYGAHENYLM
                     KRSTPFPDIVRHITPFFVSRQVICGAGRVGIGQDGSKHGFQISQRADYFEVEVGLETT
                     LKRPIVNTRDEPHADPEKYRRLHVIIGDANLAEISTYLKVGMTSLVLAMIEDRAISAD
                     LAVDGPVNELHQVSHDTSLKHLIRLKDGRELTALDIQEAYLELARGYVEDRMGSDADD
                     QTADILDRWGATIEALRRDPMECRKQLDWVAKLSLLESFRDRDRLDWDSPKLHMIDLQ
                     YADLRPDKGLYQRLLQRGSIERLLTDAEVARAVSQPPTDTRAYFRGRCLAQYPDRIAA
                     ASWDSVIFDLPGRDSLQRIPTMEPARGTRQHVGELLDRCETAEELFAAITA"
     gene            complement(5735..5950)
                     /locus_tag="VH419_00925"
     CDS             complement(5735..5950)
                     /locus_tag="VH419_00925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042207.1"
                     /translation="MTKAPHEAKTGVEAIVMMLVWWGLPLILVAALIYWAQREKPAKR
                     DSEGGVHLTEDETKLAGGPEYRHPYGA"
     gene            6003..6578
                     /locus_tag="VH419_00930"
     CDS             6003..6578
                     /locus_tag="VH419_00930"
                     /inference="COORDINATES: protein motif:HMM:NF013340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEY7042208.1"
                     /translation="MAELRAVVLVFAVAAVSTVSTVSTLSACSGGGASDTPEPTTEQQ
                     SITVTSKAFSDGDLIPETFSCDGENVSPPLDWDGVPGAAQSLALVVADPDAPGGTYYH
                     WLVTDIPVDITNIGQDSEPDGGVVADNSAGDAAYTGPCPPSGTHHYRFTIYALSKPTG
                     LPPGSGVDETVDAIGTRAIASGTLVGTYTRH"
     gene            complement(7195..8949)
                     /gene="arc"
                     /locus_tag="VH419_00935"
     CDS             complement(7195..8949)
                     /gene="arc"
                     /locus_tag="VH419_00935"
                     /EC_number="5.6.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000502 - proteasome complex [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome ATPase"
                     /protein_id="HEY7042209.1"
                     /translation="MTAHEDGRTRGSRSNEELRTQVSFLESEVSELRRRLSDAPQATR
                     FLEQRLAEAQSSLASLTSQNERLAGTLREARDQIMTLKEEVDRLAQPPTGFGTFLGKN
                     DDDTIDVFTGGRKLRVNVSPNVDVDELQRGQEVMLNEALNVVAALHFEQVGEVVMLKE
                     VLADTERALVIANADEERVVRIAAPLRDIKLRAGDSVLLDARAGYIYERIPKTEVEEL
                     VLEEVPDIDYAKIGGLSRQIDAIRDAIELPYLYPDLFQEHELKPPKGVLLYGPPGCGK
                     TLIAKAVANSLAKKVAAKTGEEGKSFFLNIKGPELLNKYVGETERHIRLVFQRAREKA
                     SEGTPVIVFFDEMDSLFRTRGSGVSSDVENTIVPQLLSEIDGVEGLENVIVIGASNRE
                     DMIDPAILRPGRLDVKIKIERPDAESARDIFSKYLTPTLPLHSDDLAEFGNNPNACVG
                     AMIQRTVERMYTESEENRFLEVTYANGDKEILYFKDFNSGAMIQNIVDRAKKMAIKDL
                     LDNDQRGLRVGHLLQACIDEFKENEDLPNTTNPDDWARISGKKGERIVFIRTLISGKE
                     GTEAGRSIDNVANTGQYL"
     gene            complement(9181..10131)
                     /locus_tag="VH419_00940"
     CDS             complement(9181..10131)
                     /locus_tag="VH419_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017935374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
                     /protein_id="HEY7042210.1"
                     /translation="MSEDVSVELQTVLSGVRRGPLRAGERVRLTDPKGRNHSVLLEAG
                     KTFFTSKGAIAHDALIGGPDGTVVTSSGGTEYLVLRPLLESFVTSMPRGAAVVYPKDA
                     AQIVTMADIFPGAKVVEAGVGSGALTCSLLRAVVPGGHVISYERRADFAEIAERNVRS
                     FFGDAHPDWTLRVGDLATDLGEREVDRVVLDMLAPWECLPAVAEALVPGGIVCGYVAT
                     TTQLSRLVEAIRGHSGFTEPYAWESIVRGWHVEGLAVRPQHHMNGHTGFLVTARRMAP
                     GRTMPKRSRRPSPGAYGPDYSGPRPAELTDEAVRPVDEDT"
BASE COUNT         1573 a         3400 c         3449 g         1747 t
ORIGIN      
        1 atcgtgatgg tgggcttctc gccgtacttc ccggacacct ggatgcggtc ctggaggccg
       61 gaggcgtcct tggggctggc ggccgagccg caggcggaga cggcgaccga gaccaggagc
      121 gctgtgacga cgaggccgac acggcgcctg tggtggggca gggtcacatt ccctcgatca
      181 gtcgttccac tcgctcgtcg tgggagcgga aggggtcctt gcacaacacg gtgcgctggg
      241 cctggtcgtt gagcttgagg tgcacccagt cgacggtgaa gtcacgacgg cgttcctgcg
      301 cccgtcggat gaactcgccg cgcaacctcg cgcgggtcgt ctggggcggt acggacttgg
      361 cctcgaagat cgccaggtcg gaggtgaccc gggccacagc gccgcgccgt tcgagcaggt
      421 agtacaggcc gcggcggcgg tggatgtcgt ggtacgccag gtcgagctgg gcgacgcgcg
      481 ccgaggacag gccgaggccg tgctgcgagc ggtaccgctc gatcagccgg tacttgatca
      541 cccagtcgat ctcgcgctcg accaggctca ggtcgtcgga ctccacagcc ttgaggccgc
      601 gctcccacag atccagggcg gcgtccaccg tcggcgtgtg cagctctcga cggtccacga
      661 agtcgcgggc cttcgcgagg tactcgttct ggatgtccag cgccgacgcg tcccgtccgt
      721 tcgccagccg aacccggcgc tgcccggtca tgtcgtggga gatctcgcgg atggcgcgga
      781 tcgggttctc catcgtcatg tcgcgcatca ccacaccggc ctcgatcatc cggagcacaa
      841 gatcggtggt cgcaaccttg agcatcgtcg tggtctcgct catgttggag tcgcccacga
      901 tgacgtgcag ccgacggaag cgctcggcgt ccgcgtgcgg ctcgtcccgg gtgttgatga
      961 tcggacgtga ccgggtggtg gcgctggaga cgccctccca gatgtgctcg gcgcgttggc
     1021 tgaccgagta gatcgcacca cgcggcgtct gcagcacctt gccggcgccg cagatgatct
     1081 gccggctgac gaggaacggg atcaggatgt cggccagccg ggcgaactca ccgtgccggc
     1141 cgaccaggta gttctcgtgg cagccgtacg agttgcccgc cgagtcggtg ttgttcttga
     1201 agaggtagac gtcgccggcg atcccctctt cgcgaagccg gcgctcggcg tccacgagca
     1261 ggccctccag gacccgctca cccgccttgt cgtgcgcgac gaggtcggtc acggagtcgc
     1321 actcgggagt ggcgtactcc gggtggctcc cgacgtcgag gtacagccgc gctccgttgc
     1381 ggaggaagac gttgctggac cgtccccacg acacgactcg gcggaagaga tagcgcgcca
     1441 cctcgtcggg tgacagccgg cgcgcaccct tgaacgtgca cgtcacgccg tactcgttct
     1501 cgatgcccaa gatccgccga tccacctgtt caccttatgt cagaacagct gggttccggc
     1561 ggccgagcga acccccgccc gttgtggacc ccggattgcg ggaagttccg gccttttcgg
     1621 cctcgaaatc ccggaagttc ccgcaattcg cgggaggcct gcgcgttcgt cagagcaggc
     1681 cggcgtggtc gaggccgagg tagacgatgt cgacgccggt catcccgccg ttgcgcagct
     1741 gctcacgaac gtcggcgacg tcgagaacga cgggttcgca gtcctcgtag gcgtcgaggt
     1801 tctggacgcc gtccggccgg cagtcacgtg cgatcacgac atgccgttga ccggtggagg
     1861 aggcgacgcg agcggtggcg accagctcca tgtcgtcact ggtgtagccg gtctcctcgg
     1921 tgagctcgcg ggccgctgcc tcgatcggcg tctcgccttc gtcgacgaag ccgcccggga
     1981 tgttgaggac cacccggtcc gggcccggtc ggaactggcg gacacacagc agccgcccgt
     2041 caggggtcaa cgcgagtacg ccgacggtct ggccaccgcc gaacatctcc cactccgcaa
     2101 cacggccgtc gggcaaccgg tacgtgcgcc tgctgacccg tatccacccc tgatggatga
     2161 ccgtgtcgtc accgacgacc tgccagtttc gtgcgtcgtc aggcatccgg ctcgtcagga
     2221 aggtcgccga tcgcatccgc cgggtcctcc gccggcgact cggtggagga ttccgggctg
     2281 gtctcctcct ccggcggcgc ggcgtcgaac agggcagcga gccgggctcc ggtgaggcga
     2341 cggaacttgc gtggctgagt gcgggtgcgg tcgaggatcg cgacctcgag gttgtcgcgg
     2401 ttgacctggc ggttggctgc ggctgcgccc tcgtggccga gtgcgtccac ggcgagccgg
     2461 agcgcggccg ccaggtcgag accctgggcg taacgggagg agaggtagtc gttgacactc
     2521 tcgaccgcgc cacccatcac ggcgtacccc tggacgtctg ccaccgagcc gtcgtacgtc
     2581 agccggtaga tctggtcctc cgctgcggtg tcaccgacct cggcgacgaa gatctcgacc
     2641 tcgtagggct tctcgccgcc ggaggagaag atggtgccga gcgtctgggc gtaggcgttg
     2701 gccaggccac ggccggtcac gtcgcgacgg tcgtaggagt agccgcgcag gtcggccagg
     2761 cggacaccgg cgatccgcag gttctcgaac tcgttgtagc ggccgaccgc ggcgaacgcg
     2821 agccggtcgt agatctcgga caccttgtgc agcgagggcg aggtgttctc ggaggcgaag
     2881 agcagcccgt cgacgtactg cagcacgacg aggctgcggc cccgggcgat ccccttccgg
     2941 gcgaagtccg cccggtcctt catcagctgc tcgggcgaga cgtagaacgg catcgacatc
     3001 gaacggtttc ctatgtgagc ggggccgagg ggccgtcggg acgctgcatc cgagccgcca
     3061 ccacctcgtc agcgatcccg gcgatctcgg agtcgccgag ccgcgagaag ccgtcctcgg
     3121 tgaccatcgc gacaaccggg aagatccgcc tggtcaggtc aggaccgccg gtggccgagt
     3181 cgtcgtcggc tgcgtcgtac aacgcctgga cggtcgccat gacggactcc cgagcagtga
     3241 ggtcgtcacg gtagagcttc ttgagcgaac cacgggcgaa gaccgagccc gagccgaccg
     3301 agtggaacgc gtgctcctcg tatcgccctc ccgtggcgtc gtaggagaag atccggccca
     3361 cgccggcatg caggtcatag ccggcgaaca gcggcacgac ggccagacct tgcatcgcca
     3421 tgccgaggtt gctgcggatc agcgcagcca accggttggc cttgccgtcc agcgacagtg
     3481 tggtgccctc gatcttctcg tagtgctcga gctcgacggc gaacagccgg atgagctcga
     3541 gcccgatccc ggccgcgccg gccatgccga cgcaggagta ctcgtcggcc gggaagacct
     3601 tctcgatgtc gcgctgggcg atgatgttgc ccatcgtggc gcgccggtcg cccgccatca
     3661 ccacgccgcc ctcgaacgtg gcggccacga gtgtcgtggc gtgtggcgtc tcgggcatgg
     3721 tgccgctcac cggcgtacgc cgtccgggca gcagctcagg cgcctgcgcc gccaggaact
     3781 cggcgaagga cgagctgccc ggcgtgacgt acgcgccggg aagtcgaggc tgtgacatcg
     3841 gctgtgacat ctctggggac aacggcgtca ctgcccaccc ttctgcacga aaccccgcac
     3901 gaactcctcg gcgttggact cgaggacctc gtcgatgtcg tcgaggatgg cgtcgacgtc
     3961 gtcgtcgagc ttctccttgc gttcggcagc agcttcgctg ggcgccgcct cgggaggtgc
     4021 gtcgtcctcc gaggacttgc gcgtggtcct gtgctgctgg ccaccttcgc gtgccatcgt
     4081 ccacctcctc tgctccactt gtcgggaatc ctgccactgt cgaccctacc cagtggcggg
     4141 gacgcgcaga tcgacgctat gccgtgatcg ccgcgaacag ctcctcggcg gtctcacagc
     4201 ggtcgagcag ttccccgacg tgctgccgcg tgccccgggc cggctccatc gtcgggatgc
     4261 gttgcagcga gtcgcggccg gggaggtcga agatcaccga gtcccatgag gcggcggcaa
     4321 tccggtcggg gtactgcgcg aggcagcggc cgcggaagta cgcgcgcgtg tcggtgggcg
     4381 gctggctgac ggccctggcc acctcggcat cggtgaggag gcgctcgatc gaaccacgtt
     4441 ggagcagccg ctggtacaga cccttgtccg gacgcaggtc ggcgtactgg aggtcgatca
     4501 tgtgcagctt cgggctgtcc cagtcgagcc ggtcgcggtc gcggaaggac tccagcagcg
     4561 acagcttcgc gacccagtcg agctgcttgc ggcattccat cgggtctcgc cgcagcgcct
     4621 cgatggtcgc accccaccgg tccaggatgt cggcggtctg gtcgtcggcg tcggaaccca
     4681 tccggtcctc gacgtagccg cgggcaagct cgaggtacgc ctcctggatg tcgagcgcgg
     4741 tcagctcgcg accgtccttc aagcggatca ggtgcttgag gctggtgtca tgggagacct
     4801 ggtggagctc gttgacgggc ccgtcgacgg cgagatccgc gctgatcgcc cggtcctcga
     4861 tcatcgcgag caccaacgag gtcatcccga ccttcaggta cgtcgagatc tcggccaggt
     4921 tcgcgtcgcc gatgatgacg tggagccgtc ggtacttctc ggggtcggcg tgtggctcgt
     4981 cacgggtgtt gacgatcgga cgcttcagcg tggtctcgag cccgacctcc acctcgaagt
     5041 agtcggcccg ctggctgatc tggaagccgt gtttgctgcc gtcctggccg atcccgaccc
     5101 ggccggcccc acagatcacc tgccggctga cgaagaacgg tgtgatgtgc cggacgatgt
     5161 cggggaaggg cgtcgaccgc ttcatgaggt agttctcgtg cgccccgtag gacgcgccct
     5221 tgttgtcggt gttgttcttg tagagcacga gcgggttctg gcccgggatg tcgaccgcgg
     5281 ccgcggcggc ggcgatgatg agctcccccg ccttgtccca cagcacgatg tcgcgcgggt
     5341 tggtgcactc aggcgtcgag tactccgggt gggcgtggtc gacgtacagc cgtgcaccgt
     5401 tggtgaggat gaggttcgcc agcccgatct cctcgtcggt caactggctc ggatcggcag
     5461 cactgcggct caggtcgaag ccccgcgcat cacgcagcgg gctctcctcc tcgaagtccc
     5521 agcgcgcgcg tcgggaccgc tggctggtgg aggcatacgc gttgacgagc tgcgaggacg
     5581 tcagcatcgg gttggctgac ggcgtgccga ggacatggat gccgaactcg gtctccgtcc
     5641 ccatgatgcg gcgtaccgtc atgctggcag cctacgggca cgctgggaac ggtggtcgcc
     5701 aaccggtgac gtcccggcgg cgctgcagtt cggttcaggc gccgtacgga tgccggtact
     5761 cgggtccgcc ggccagcttg gtctcgtcct cggtgaggtg gacgccgcct tctgagtcgc
     5821 gcttggccgg cttctcgcgc tgggcccagt agatgagcgc cgcgaccagg atcaggggta
     5881 ggccccacca caccagcatc atcacgatcg cctccacacc agtcttggcc tcatgcgggg
     5941 ccttcgtcac cagggtaccg aggtgtcggc tgttcagccg ctcgacgtga cacgatgctg
     6001 ccgtggcgga attacgggcc gtcgtcctcg tcttcgccgt ggcggccgtg tcgacggtgt
     6061 cgacggtgtc cacgctgtcc gcgtgcagcg gaggcggtgc gtcagacaca ccggagccga
     6121 cgaccgaaca gcagtcgatc acggtcacga gcaaggcgtt ctcggacggc gatctcatcc
     6181 cggagacgtt cagctgcgac ggtgagaacg tctcgccgcc gctggactgg gacggcgtac
     6241 cgggtgccgc gcagtccctc gccctcgtcg tggcggatcc ggacgcgccg ggagggacgt
     6301 actaccactg gctcgtgacg gacattcccg tcgacatcac caacatcggc caggacagcg
     6361 agcccgacgg cggtgtggtg gcggacaact cggccggcga cgcggcatac accgggccgt
     6421 gtccgcccag cgggactcat cactaccggt tcacgatcta cgcgctgtcg aagccgaccg
     6481 gactcccgcc gggctcaggg gtcgacgaaa ccgtcgacgc gatcggcacc cgtgccatcg
     6541 caagcggcac gctggtcggc acctacaccc gccactgaca gctggtgggt ttgcctcggt
     6601 agccatccaa acggacgggt agccaggcag gtctgcctcg ggacgggtgg gcttgcctgg
     6661 gtggccatgc aaaccgccgc tcagttccgc cggtggtccg cgatgttcac atcccaaacc
     6721 ggctccatcc atccgaggtt cgcgatgttc acacggcact tgggctgtaa acatcgcaaa
     6781 ccaccacggt tgggtgtcgg tgagggtttg cctcggtagc catccaaacg gccgggtagc
     6841 caggcaggtc tgcctcggga gggatgggct tgcctgggta gccatgcaaa ccgccgcccg
     6901 gcgacccgtg gatgtccgaa acccgccagt cacacaccga ccaacaaggg acggaaaccc
     6961 ggccggccag ctcagcaggc acaaacggtt cccgcccttc gccgccaatc acctcagcca
     7021 gcacgacacg acactgcgcc gggcaacgac gccaacccaa cacgaccgtc tccgccccac
     7081 cgaaaccgca ggagagccca gatctgcctg gctacccatc cggttgcacg gctagccagg
     7141 cagaccacga cggtcaccta tcggttgcgg acgtggccgg cagaaaccag agggtcagag
     7201 gtattggccg gtgttggcga cattgtcgat ggagcgaccg gcttcggtac cttccttgcc
     7261 ggagatgagg gtgcggatga acacgatgcg ctcacccttc ttgccggaga tccgggccca
     7321 gtcgtccggg ttggtcgtgt tcggcaggtc ctcgttctcc ttgaactcat cgatgcacgc
     7381 ctgcaggaga tgcccgacgc ggagcccccg ttggtcgttg tcgagaaggt ccttgatcgc
     7441 catcttcttg gcccgatcga cgatgttctg gatcatcgcg ccggagttga agtccttgaa
     7501 gtagaggatt tccttgtcgc cgttggcgta ggtgacctcg aggaagcggt tctcctccga
     7561 ctcggtgtac atccgttcga cggtccgctg gatcatggcg ccgacgcacg cgttcgggtt
     7621 gttgccgaac tccgccaggt cgtcggagtg cagcgggagc gtcggcgtca ggtacttcga
     7681 gaagatgtcg cgggcggact cggcatccgg gcgctcgatc ttgatcttca cgtccaaccg
     7741 gccgggccgc aggatcgccg ggtcgatcat gtcctcacgg ttggaggcgc cgatcacgat
     7801 gacgttctcg agaccttcga ccccgtcgat ctcactgagc agctgcggga cgatggtgtt
     7861 ctcgacatcg gacgagacgc ccgagccgcg ggtcctgaag agcgagtcca tctcgtcgaa
     7921 gaagacgatg accggcgtac cctcgctggc cttctcacga gcacgctgga agacgagccg
     7981 gatgtgccgc tcggtctcac cgacgtactt gttgagcagc tcgggaccct tgatgttgag
     8041 gaagaacgac ttgccctcct cacccgtctt cgcggcgacc ttcttggcca gcgagttggc
     8101 caccgccttg gcgatgagcg tcttgccgca accgggtggg ccgtacagga ggacgccctt
     8161 cggcggcttc agctcgtgct cctggaacag gtccgggtag aggtagggca gctcgatggc
     8221 gtcgcggatg gcatcgatct gccggctcag cccaccgatc ttggcgtagt cgatgtcggg
     8281 gacctcttcg agcacgagct cctcgacctc ggtcttcggg atccgctcgt agatgtatcc
     8341 cgctcgcgcg tccagcaaca ccgagtcacc ggctcgcagc ttgatgtccc gcaacggggc
     8401 ggcgatccgg acgacgcgct cctcatcggc gttcgcgatg accagggcac gctcggtgtc
     8461 ggcgagcacc tccttcagca tcaccacctc gcccacctgc tcgaagtgca acgcagcgac
     8521 gacgttcagc gcctcgttca gcatgacctc ctggccacgc tgcagctcgt cgacgtcgac
     8581 attcgggctg acgttgacgc gcagcttccg gccgccggtg aacacgtcga tcgtgtcgtc
     8641 gtcgttcttg ccgaggaacg tcccgaagcc ggtcggtggc tgggcgagcc ggtcgacctc
     8701 ctccttcagc gtcatgatct ggtcgcgggc ctcacgcaga gtgccggcga gacgttcgtt
     8761 ctgcgacgtc agcgaggcga gcgaggactg ggcctcggcg agacgctgct ccaggaaccg
     8821 ggtggcttgc ggtgcatcgg acaggcggcg gcggagttcg gacacctcgg actcgaggaa
     8881 gctgacctgc gtgcgcagct cctcgttcga tctggatcct cgagttcgtc cgtcttcgtg
     8941 tgcagtcacc aaaaccacct ccgctgggag gactgctgaa tccaacctac ccgcgtaggg
     9001 agtcggaagg gtcaggtctg gatacgtgtc ggtatcagta tgtgatcttg accgcgatgt
     9061 ctgtccggcc agccgacccc cggcgtcggc cgaacgcacc acccggtcgt ggttgtgctc
     9121 cgctggggct tcctcacccg gagttcggcg gggaagccgt cgcggatgga gcgtggcggg
     9181 tcacgtgtcc tcgtcgaccg gtcgcacagc ctcgtcggtg agctcggccg gacgcgggcc
     9241 gctgtagtcc ggcccgtacg cgcctggcga gggtcgccgg ctccgcttcg gcatcgtccg
     9301 ccctggcgcc atccgacggg ccgtgaccag gaatccggta tgcccgttca tgtggtgctg
     9361 cggccgtacc gcgagaccct cgacgtgcca tccacgcacg atcgactccc aagcgtacgg
     9421 ctcggtgaag ccactgtgcc cgcggatcgc ctccaccaga cggctgagct gtgtcgtcgt
     9481 ggcgacgtac ccgcacacga tcccgcccgg caccaacgcc tccgcgacgg ccgggagaca
     9541 ttcccaggga gccagcatgt ccaggacgac gcggtccacc tcgcgttcgc ccaggtcggt
     9601 cgccaagtcg ccgactcgga gcgtccagtc cgggtgagcg tcaccgaaga acgatcggac
     9661 gttgcgctcg gcgatctcgg cgaagtccgc tcgtcgctcg tacgagatga cgtgaccacc
     9721 cggaaccacg gcacgcagca gcgaacaggt caacgcaccc gaaccgaccc ccgcctcgac
     9781 gactttcgcg cccgggaaga tgtcggccat cgtcacgatc tgcgcggcgt ccttcgggta
     9841 gaccacggcg gcgccgcgtg gcatcgacgt gacgaagctc tccagcagcg gtcgcagcac
     9901 gagatactcc gtgccgccgc tggacgtcac caccgtgccg tccggaccac cgatcagcgc
     9961 gtcatgcgcg atcgcgccct tggacgtgaa gaacgtcttc cccgcctcga gcaacacgct
    10021 gtggttgcgg cccttcgggt cggtgagccg tacccgctcc cccgcccgca gcggcccacg
    10081 gcgtacgccg gacagcacgg tctgcagctc gacgctgacg tcctcggaca tggccgtcac
    10141 gttagtggct cacgccattc ggaaggcag
//
LOCUS       DATBFZ010000030         2750 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_3299777, whole genome shotgun sequence.
ACCESSION   DATBFZ010000030 DATBFZ010000000
VERSION     DATBFZ010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2750
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3299777"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..690)
                     /locus_tag="VH419_00945"
     CDS             complement(<1..690)
                     /locus_tag="VH419_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007077109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
                     /protein_id="HEY7042211.1"
                     /translation="MAGQDLSDRLKQLDATLSNIERVLDLPGMRAEIGELGEQVAAPD
                     LWDDQANAQRVTGRLSLLQAELERVSSLRQRLDDTEVLIELANEEDDAGSLKEAETEL
                     DRLAKSIEALEVRTLLSGEYDEREALVSVRSGAGGVDAADWAEMLMRMYLRWAERHKF
                     PTEVYETSYAEEAGIKSATFAVKAPFAYGTLSVEQGTHRLVRISPFDNQGRRQTSFAA
                     VEVVPVLEQTDD"
     gene            complement(734..1012)
                     /locus_tag="VH419_00950"
     CDS             complement(734..1012)
                     /locus_tag="VH419_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042212.1"
                     /translation="MTELPRDMDDAAPREADVERAQEKERVDLDPEDQPNAPNREPVT
                     ERVGPNADPDQVPGPLEPGENAPDRPDDVTSFEQPGGAGNWDDQDMED"
     gene            complement(1111..2172)
                     /locus_tag="VH419_00955"
     CDS             complement(1111..2172)
                     /locus_tag="VH419_00955"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY7042213.1"
                     /translation="MTAFRSLARAMLKSFLRDRQAVFFSVFFPLMFLVLFGGIFANQS
                     QSRISMVQVGDVSLIDNLPREARAAFDDSFDVEKSDDLDAAIQKVRDGDVTIAVQQQG
                     DQIIAHYSDADQVQSAVAQGTLRSFIQSANVAASGKPPTYTYAGERVEDESLKTIQFV
                     TPGLLGWAIATSATFGAAVTLVGWRTTRLLRRIRLSPVTTTSVVAARIGVTMLIALVQ
                     MAIFVGLGVVAFGLQLTGSWYMAVPLLICGTLAFMALGLFAGSVSKTVEGATSLAQVF
                     VLPMAFLSGSFFSLDGAPPWLNVTSHVLPLRYLNDGMLDVMVRGLGPSAAVVPMLVLL
                     GFAAVVIAISSRFFSWDVE"
     gene            complement(2169..>2750)
                     /locus_tag="VH419_00960"
     CDS             complement(2169..>2750)
                     /locus_tag="VH419_00960"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY7042214.1"
                     /translation="AGAAADEWLERVGMTDKADTRADKLSGGQLQRLSIACALVHEPE
                     IVFLDEPTAALDPQARRNLWDLLATLNDHGRTVVLTTHYMDEAEILCDRVAIMDLGKI
                     LELDTPAALIRALDAGVRISVDLSAIAEADARSLPGVEHVDVDTTSVVLSTRDPAAVL
                     SGLAHREALAGLQVKGATLEDVFLTLTGREYRA"
BASE COUNT          444 a          941 c          911 g          454 t
ORIGIN      
        1 gtcgtcggtc tgctccagca ccggtacgac ctcgaccgcc gcgaacgagg tctggcgtcg
       61 tccttggttg tcgaacggtg agatacggac gaggcggtgg gtgccctgct cgacgctcag
      121 cgtgccgtag gcgaagggtg ccttgaccgc gaaggtggcc gacttgattc ccgcctcttc
      181 ggcgtacgac gtctcgtaga cctcggtggg gaacttgtgg cgctcggccc agcgcaggta
      241 catccgcatg agcatctcgg cccagtccgc ggcgtccact cccccggctc cggatcggac
      301 gctgaccagc gcctcgcgct cgtcgtactc gccggacagc aaggtgcgga cctcgagcgc
      361 ctcgatgctc ttcgccagcc ggtcgagctc ggtctcggcc tccttcaggg agccggcgtc
      421 gtcctcttcg ttcgcgagct cgatgagcac ctcggtgtcg tcgagacgtt gccgcaggct
      481 cgacacccgc tcgagctccg cctggagcag cgacagccgg ccggtgactc gctgggcgtt
      541 cgcctggtcg tcccacaggt ccggtgcggc gacctgctcg ccgagctcac cgatctccgc
      601 ccgcatcccc ggcaggtcga gcacccgctc gatgttgctc aacgtcgcgt cgagctgttt
      661 gagccggtcg gagaggtcct gtcctgccac gggccaaggg tacggcgtgc cgcgaaccgg
      721 ccgcgaaccc cggtcagtcc tccatgtcct ggtcgtccca gttgccggcg ccacccggct
      781 gctcgaacga cgtcacgtcg tcagggcgat ccggcgcgtt ctctcctggc tccaacgggc
      841 cgggcacctg gtccgggtcg gcgttcggcc cgacccgctc ggtcaccggc tcacggttgg
      901 gcgcgttggg ttgatcctcc gggtcgaggt cgacccgctc tttctcctgg gctcgttcga
      961 cgtccgcctc acgcggtgcg gcgtcgtcca tgtctcgcgg cagctcggtc atggagccgc
     1021 cgtaccctcc agcgaagtga aggaaacgtc agggttctca ggtggcttct tgagccgcgc
     1081 cccagcggct gcgccgctgc ggcgcgtcca tcattcgacg tcccagctga agaagcggct
     1141 ggagatggcg atcacgacgg cggcgaagcc gagcaggacc agcatcggga cgacggcggc
     1201 gctcggaccg aggccgcgga ccatgacgtc gagcatcccg tcgttcagat agcgcagcgg
     1261 cagcacgtgt gaggtgacgt tcagccacgg cggcgcaccg tcgagagaga agaacgaccc
     1321 ggagaggaat gccatcggca gcacgaacac ctgggccagt gacgtggcgc cctcgaccgt
     1381 cttggacacc gacccggcga acaacccgag cgccatgaac gccaacgtgc cgcagatcag
     1441 caacggcacg gccatgtacc acgagccggt cagctggagc ccgaacgcca cgacccccag
     1501 ccccacgaag atcgccatct ggaccaacgc gatcagcatc gtgacgccga tgcgagcagc
     1561 caccacggac gtggtggtca ccggcgaaag tctgatccgg cgcagcagcc tcgtggtccg
     1621 ccatcccacc aaggtgaccg cagcgccgaa cgtcgcgctc gtggcgattg cccatccgag
     1681 cagacctggt gtgacgaact ggatcgtctt cagggactcg tcctccacgc gttcaccggc
     1741 gtacgtgtag gtcggaggtt tgccggaggc cgcgacgttc gcggactgga tgaaggaccg
     1801 gagcgtgccc tgcgcgacgg cggactgcac ctggtcggca tcgctgtagt gggcgatgat
     1861 ctggtcgccc tgctgctgga ccgcgatcgt cacatcgccg tcgcgaacct tctggatcgc
     1921 cgcatcgagg tcatctgact tctctacgtc gaacgagtcg tcgaacgccg ccctcgcctc
     1981 tctcggaagg ttgtcgatca gcgacacgtc gccgacctgc accatggaga tcctgctctg
     2041 tgactggttg gcgaagatcc ccccgaacag aaccaggaac atcagcggga agaacaccga
     2101 gaagaacacc gcttgtcggt cccgcaggaa gctcttgagc atcgcacggg cgaggctgcg
     2161 aaaggcggtc atgcccggta ctcccggccg gtcagcgtga ggaagacgtc ctcgagcgtg
     2221 gctcccttga cctgaagtcc ggcgagcgcc tcgcggtgcg ccaggcccga cagtacggcg
     2281 gccggatctc gcgtcgacaa cacgaccgac gtcgtatcca cgtcgacgtg ctcgacaccg
     2341 gggagcgacc gggcatccgc ttcggcgatc gcagacaggt cgacactgat gcgcactcct
     2401 gcatcgagtg cacgtatcag cgccgcaggg gtgtcgagct cgaggatctt gccgaggtcc
     2461 atgatcgcga cccgatcaca caagatctcg gcttcatcca tgtagtgcgt ggtgagcacg
     2521 acggtacgtc cgtggtcgtt gagggtggcg agcagatccc acaggttgcg tcgggcctgc
     2581 gggtcgagcg cggcggtcgg ctcgtcgagg aagacgatct ccggctcgtg cacgagcgcg
     2641 caggcgatcg agagccgttg caactggccg cccgagagct tgtcggcccg cgtgtcggcc
     2701 ttgtccgtca taccgacgcg ttcgagccac tcgtccgccg cggcgccggc
//
LOCUS       DATBFZ010000031         2644 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_19236, whole genome shotgun sequence.
ACCESSION   DATBFZ010000031 DATBFZ010000000
VERSION     DATBFZ010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2644
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19236"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..742)
                     /locus_tag="VH419_00965"
     CDS             complement(<1..742)
                     /locus_tag="VH419_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HEY7042215.1"
                     /translation="MVTSSVTEGRASLTRTWRPDWPCDPYDVLRPLRRGAGDPTFQRD
                     PDGTVWRASMTPEGPATVRISRRAADGEIDLEAWGQGATWMLEQAPAMLGAHDDVTGF
                     RSDDPVVRTAWQRNPHWRVPTSGLVLEALVAAVVEQKVTGQEAWTGWRRLVRRFGVPA
                     PGPGLDRGMWTLPTAETIARIPSWEWLRCHIDGGRSRTIVRAARVAPSLERTVGLPMD
                     AVERRLRSLLGVGVWTAAEVRQRAHGDAD"
     gene            867..1253
                     /locus_tag="VH419_00970"
     CDS             867..1253
                     /locus_tag="VH419_00970"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY7042216.1"
                     /translation="MTEPIRVIRHAELAAADPTPGMMRKRAFELPKLWAGQVETAPGA
                     VSGWHHHDVNETSLYVVSGVLRLEFEGYDGYVDAQAGDFIHVPAFTVHRESNPTDVPS
                     VAVIARAGGGIPTVNVDPPPTHRVDD"
     gene            1378..>2644
                     /locus_tag="VH419_00975"
     CDS             1378..>2644
                     /locus_tag="VH419_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEY7042217.1"
                     /translation="MAGLMLADVVATSQQVTATRSRLAKRTALVELLRRADAADIEVA
                     VAFMSGVLRQRRTGLGWASLRDLPPPADTATLTIAEVDAAFEAISRLAGTGSATARGA
                     AATALMGRATADEQAFLRGLVYGGLRQGALETSLVDAVADASGVPLAAVRRAVMLRGA
                     VGPVATAAMTEGQGAVEAFTLTVGQPIKPMLASTAPDIAAAFDKVGAGDDSTKTCVDT
                     KLDGIRIQVHKQGPDVSVFTRSLDDITARLPEVVTVTTALPVDRAVVDGEAIALDQSG
                     RPRPFQETSSRTATQLDEIAGPPGTDVTPFFFDCLHLDGEDLIDLPLRERLARLDAVT
                     PESARIRRLVTASAEAADTFFLDQVAAGQEGVVVKDLEAPYDAGRRGAAWVKVKPRHT
                     LDLVVLAVEWGSGRRQGWLSNIHMGARDPD"
BASE COUNT          384 a          979 c          916 g          365 t
ORIGIN      
        1 cgtcggcatc tccgtgcgcc cgctggcgca cctcggccgc cgtccacacg ccgacgccga
       61 gcagcgagcg taggcggcgc tcgactgcgt ccatcggcag gccgacggtg cgctcgaggg
      121 acggcgccac tcgggcggcc cgcacgatcg tccgcgacct gccaccgtcg atgtggcagc
      181 gcagccactc ccacgacggg atgcgtgcga tcgtctcggc ggtcgggagt gtccacatgc
      241 cacggtcgag gccgggtccg ggcgccggca cgccgaatcg ccggaccagc cgtcgccagc
      301 cggtccaggc ctcctggccg gtgaccttct gctccaccac ggcggcgacc agtgcctcga
      361 gcacgagccc gctcgtcggg acccgccagt gcgggttgcg ctgccaggcc gtgcggacga
      421 ccgggtcgtc gctgcggaag cccgtcacgt cgtcgtgggc gccgagcatc gcgggtgcct
      481 gttcgagcat ccatgtcgcg ccctggcccc acgcctccag gtcgatctca ccgtcggcgg
      541 cgcgccggct gatccgcacg gtggccggcc cctcgggtgt catcgaggcg cgccacacgg
      601 tgccgtccgg gtctcgttgg aacgtcgggt caccggctcc gcggcgcagc gggcgcagca
      661 cgtcgtacgg atcacacggc cagtcaggcc gccaggtccg cgtgagcgac gcccgtccct
      721 ccgtcacgct cgacgtcacc atcgccactc ccccacccta tgcgcccgac ctctcgccga
      781 ggtggcaggt ttcgttcacg acacgccgca tcgcgaacgt cgatcgccac ctcggcgtac
      841 gtccccgaca gcacgagaat ggctgcatga ccgaaccgat ccgggtgatc cgacacgcag
      901 agctggccgc agccgatccc acgcccggca tgatgcgcaa acgggccttc gagctgccga
      961 agctgtgggc cggtcaggtc gagaccgcac ccggcgcggt ctccggctgg caccaccacg
     1021 acgtcaacga gacgagcctg tacgtcgtgt ccggcgttct gcgactcgag ttcgaggggt
     1081 acgacgggta cgtcgacgcc caggcgggcg acttcatcca cgtccccgct ttcacggtgc
     1141 atcgagagag caacccgacc gacgtacctt ccgttgcggt gatcgcgcga gccggcggtg
     1201 gcatccccac cgtcaacgtc gacccaccac cgactcatcg cgtcgacgac tgaggcggac
     1261 tcgccggcgt tcaaggtcgg tcgaggtggc aatcgcagta cgacacgccg acagaaccca
     1321 acgaaacctg ccacctcggc gggagggggt gtcggagcgg ggcggcagac tgggcgggtg
     1381 gcgggactga tgctggcgga cgtggtggcc acctcgcagc aggtgacggc gacccgttcg
     1441 cggctggcga agcgtacggc gttggtggag ctcctgcgcc gggcggacgc cgccgacatc
     1501 gaggtcgcgg tggcgttcat gtccggcgta ctccgtcaac gccgcaccgg gctggggtgg
     1561 gcctcgctgc gagacctgcc gcctccggcc gacaccgcca cgctgacgat cgccgaggtc
     1621 gacgccgcct tcgaggccat cagcaggctg gccggcaccg ggtcggcaac ggctcgcggc
     1681 gcagcggcga cggcgttgat gggccgcgcc actgcggacg aacaggcctt cctccgaggt
     1741 ctcgtgtacg gcgggctcag acagggcgcg ctcgaaacat ccctggtcga cgcggtcgcg
     1801 gatgcgtccg gtgtccccct cgctgccgta cgacgtgccg tgatgctccg cggcgccgtc
     1861 ggcccggtcg ccacggcggc gatgaccgaa gggcagggcg cggtcgaggc gttcacgctc
     1921 accgtcggac agccgatcaa gccgatgctc gcgtccaccg ctcccgacat cgcggccgcc
     1981 ttcgacaagg tcggcgcggg tgacgactcg acgaagacct gcgtcgacac caagctcgac
     2041 ggcatccgca tccaggtgca caagcagggc cccgacgtct cggtgttcac ccgcagcctc
     2101 gacgacatca ccgcgcgact gccggaggtg gtgacggtga cgacagcact gcccgtcgac
     2161 cgggcggttg tggacgggga ggcgatcgcg ctggaccagt ccgggcgtcc ccggcccttc
     2221 caggagacgt cgtcacggac cgccacgcag ctcgacgaga tcgccgggcc gcccgggacc
     2281 gacgtcacgc cgttcttctt cgactgcctc cacctcgacg gcgaggacct catcgacctg
     2341 ccgctacgcg aacggctggc tcggctcgac gccgtcacgc cggagtcggc ccgcatccgc
     2401 cggctggtca ccgcctccgc tgaggccgcc gacaccttct tcctcgacca ggtggccgca
     2461 ggtcaggaag gcgtcgtggt caaggacctc gaggcgccgt acgacgcggg ccgtcgtggc
     2521 gccgcctggg tcaaggtcaa gccgcgccac accctcgacc tggtcgtgct cgcggtcgag
     2581 tggggcagcg gacgccgcca gggttggctg tccaacatcc acatgggcgc gcgggacccc
     2641 gacg
//
LOCUS       DATBFZ010000032         6299 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_1004422, whole genome shotgun sequence.
ACCESSION   DATBFZ010000032 DATBFZ010000000
VERSION     DATBFZ010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 6299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6299
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1004422"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..457
                     /locus_tag="VH419_00980"
     CDS             <1..457
                     /locus_tag="VH419_00980"
                     /inference="COORDINATES: protein motif:HMM:TIGR02983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SigE family RNA polymerase sigma factor"
                     /protein_id="HEY7042218.1"
                     /translation="SAEDLLQTTLATVYVRWSRIRDRRAADAYMRRAMVNQQISWFRQ
                     KWRTCERPTDDLPERPHDWDHDSHHRWDRGELWPFVTALPARQRAVVVLRYYEDLSEA
                     ETARVLGCSIGTVKSQTNSGLAKLRRMARESGLGTAGVPGAGEAEARLG"
     gene            457..954
                     /locus_tag="VH419_00985"
     CDS             457..954
                     /locus_tag="VH419_00985"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY7042219.1"
                     /translation="MTTWLPADFVHPRWVDLPFGHHLRPIRESDVDLDMIAVMGSQAR
                     LWDIYGRAWGWPPATMTYEQDREDLARHAAEMETHESFNYALFDADETALLGCVYIDP
                     PEKPGADTEISWWVVDECVDTDVEAGLDAEVPRWIASSWPFERPRYVGRDLSWDEWLA
                     LPDLS"
     gene            1193..1708
                     /locus_tag="VH419_00990"
     CDS             1193..1708
                     /locus_tag="VH419_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012920607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEY7042220.1"
                     /translation="MIWTAPDVDLPDGPLTGDERPMLEGFLRWHRAYLRHKCAGLTGE
                     QLAECSAPPSNLSLLGLVRHMAKVERRWFRERIAGEELEPMYDPGLGDDADFEDLDPA
                     GAQADYERLVEECALAEDALAAAAYDDTIMSRHGPMSVRAVVVHMIEEYAQHNGHADL
                     LRERVDGTTDK"
     gene            complement(1824..2393)
                     /locus_tag="VH419_00995"
     CDS             complement(1824..2393)
                     /locus_tag="VH419_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015068958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042221.1"
                     /translation="MSNQMKKDLRLPDPDFVHPEVVAPETRSMKTARYVSVAVRLSLG
                     WVFLWAFLDKTFGLGHETAKADAWIRGGNPTEGFLAFAATGPFEGFYHNIAGQAWTDW
                     LFMLGLLGIGLALVFGVAMRIAAAAGALLLVMMWTVVLPPANNLFMDDHLIYAMVLVM
                     LAAMGAGRTFGLGRYWEKVPLVNRYPWLR"
     gene            complement(2774..3433)
                     /locus_tag="VH419_01000"
     CDS             complement(2774..3433)
                     /locus_tag="VH419_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008361416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY7042222.1"
                     /translation="MTDTSARAEKIRVFLLDDHEVVRRGLRELLEQDGDIEVVGESGL
                     AEEATRRIPALRPDVAVLDGRLPDGSGIDVCREIRSRHPEIAALILTSFDDDEALFSA
                     IMAGAAGYVLKQIRGNDLVDTVRRVADGQSMLDPAVTQQVLDRLRKGPEEDKALAPLT
                     AQERRILQHIGEGMTNRQIAETMFLAEKTVKNYVSSLLAKLGMERRTQAAVFAAKHLG
                     I"
     gene            complement(3519..5186)
                     /locus_tag="VH419_01005"
     CDS             complement(3519..5186)
                     /locus_tag="VH419_01005"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HEY7042223.1"
                     /translation="MGTSRGHEAADGTDRRPHSPLLDAVVGIGSDLSLDGVLQRIIEA
                     ARQLTGARYAALGVIGSERDKRLREFVYVGIDDEQRRLIGDLPHGLGVLGLLIDHPEP
                     LRLHDITTHPASFGFPPDHPVMRSFLGVPIRVQGRVFGNLYLSEKASGEDFDEADEDV
                     VLALAAAAGVAIDNARLYGEAARRETWLQAAAEVVAALLGPGEPQVAFDLVARRAAEQ
                     SAADHAMVVVTDEDGKLVVRAVAGELPHIVPGMALARDRTLSAVVLETGRTLVVDDVA
                     ADPRIGNELPSSEDLLSLGAVLVVPMRTPDGDEGALSMGWFKEHTSQLRDIDVPVIER
                     YAEQAALAIQVARSRETQQRLAVLEDRDRIARDLHDLVIQQLFAVGLSLESTVRITAK
                     PEVAERLVTAVDDIDSTIKDIRRTIFHLSAPEQVRDLRHDIDDVVTRSAQSLGFTPAL
                     RTTGPVAHGVPDQIAPHLLAVLGEALSNVARHARASQVTVEIEVGDAALTLVVRDDGV
                     GLDVSGGDEGGHGLANMRDRAEDLGGELGIESTPGAGSCLCWRVPVS"
     gene            5357..6289
                     /locus_tag="VH419_01010"
     CDS             5357..6289
                     /locus_tag="VH419_01010"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY7042224.1"
                     /translation="MTTQTQPTDESSTSVPVGVVIVGVDGSDKNQAALEWADREADRL
                     GYRLVLVGATPIFGTPTWGFTRDYGFAELEQDMRDQLDATRARLERPGRDIRVDAATG
                     DATRVLLEAGKHCDLLIVGKRGIGATKRMLVGSTSTAVAGRSPVPVVVVPDSWDQSRQ
                     ESAPIVVGVTDAERDHEVLEFGFTRADRLGVPLIAAYVWDLPLQVVWSPDDVARWKAE
                     ARELLDGVVAQWSKRFPDVEVVSLTPHGNVAMALVDAGEAAQLVVVGRHTGPRHLGGF
                     SLGSKTRALLHYAEFPVAVVPTKHVKSDPLADLG"
BASE COUNT         1020 a         2168 c         2130 g          981 t
ORIGIN      
        1 ctcggccgag gacctgctcc agacaaccct cgccaccgtc tacgtgcgct ggtcgcggat
       61 ccgcgaccgc cgcgcggcgg acgcctacat gcgccgggcg atggtcaacc agcagatcag
      121 ctggttccga cagaagtggc ggacctgcga gcgtccgacc gatgacctgc ccgaacgacc
      181 ccacgactgg gaccacgact cgcaccaccg ttgggaccgt ggcgagctct ggccgttcgt
      241 cacggccctc ccggcgcggc agcgagccgt cgtcgtcctc cggtactacg aagacctgtc
      301 ggaggcggag acggcgcggg tcctgggctg ctcgatcgga accgtcaagt cgcaaaccaa
      361 cagcggactg gcgaagctgc gtcggatggc ccgggagtcg ggcctcggga cagccggggt
      421 gccgggcgcc ggcgaagcgg aagcgagact gggctgatga ccacatggct gcccgcagac
      481 ttcgtacacc ctcgctgggt cgatctgccg ttcggtcacc acctgcgacc catccgcgag
      541 agcgacgtcg acctagacat gatcgccgtg atgggttcac aggcacggct gtgggacatc
      601 tacgggcgag catggggctg gccgcccgcc acgatgacgt acgagcagga ccgcgaggac
      661 ctggcccggc acgccgcgga gatggagaca cacgagtcgt tcaactacgc gttgttcgac
      721 gcggacgaga cggcgctgct gggttgtgtg tacatcgacc ctcccgagaa accaggggcg
      781 gacaccgaga tctcgtggtg ggtggtcgac gaatgcgtcg acaccgatgt cgaggcgggt
      841 ctcgacgccg aggtgccgag gtggatcgcc agcagctggc cgttcgagcg gccacggtac
      901 gtcggtcgcg acctcagctg ggacgagtgg ctggcgctcc ctgacctttc gtgagcacct
      961 ggccggcggg tgggcctcag agcctgccat gcggggatgg actcctcctc cctgctgggc
     1021 ggacatcgca aacccactgt gcccatcgga ggcttgcgat gttcacacgc cagttgggct
     1081 gtaaacatcg caaaccttcc acgggaagct ggtagagcgg ctgatcgccc agcagcacct
     1141 cacaccctca cctcgcggcg gtgcggcaca tggcagatgt gatgatggcg cgatgatctg
     1201 gacggctcct gacgtcgacc tgcccgacgg cccactcacc ggcgacgagc gaccgatgct
     1261 cgagggcttc ctgcgctggc accgcgcgta cctgcgccac aagtgcgcag gcctgaccgg
     1321 cgagcagctc gcggagtgct cggcgccgcc gtccaacctg tcgctgctcg gcctggtgcg
     1381 acacatggcc aaggtcgagc gccgctggtt ccgggagcgg atcgccggcg aggagctcga
     1441 gccgatgtac gacccgggcc tcggggacga cgccgacttc gaggatctcg acccggctgg
     1501 cgcgcaggcc gactacgagc gcctcgtcga ggagtgcgcg ctggccgagg acgcgctggc
     1561 cgcggcggcg tacgacgaca cgatcatgtc gcgccacggc ccgatgtccg tacgcgctgt
     1621 ggtcgtccac atgatcgagg agtacgccca gcacaacggg cacgccgacc tgttgcggga
     1681 gcgcgtcgac ggtacgacgg acaagtagat cgagggtacg agcgacgaag gcgacgtgat
     1741 cggccggcct cgaacgaagc tggcatgcga agaggccggg agcccgcggg cgggcgggtt
     1801 cccggcctct gtgctgggga gcgttaccgc agccaggggt agcgattgac cagcggtacc
     1861 ttctcccagt accggccgag accgaacgtc cggccggctc ccatcgccgc cagcatcacc
     1921 aggaccatgg cgtagatgag gtggtcgtcc atgaacaggt tgttcgcggg gggtaggacg
     1981 accgtccaca tcatcacgag cagcagcgca ccggccgcag ccgcgattcg catcgcgact
     2041 ccaaacacca gggccaatcc gatgccgagg agtccgagca tgaacagcca gtcggtccag
     2101 gcctgtccgg cgatgttgtg gtagaagccc tcgaagggtc cggtcgctgc aaaggccagg
     2161 aagccttcgg tcgggttgcc accacggatc catgcgtcag ccttggcggt ctcgtgaccc
     2221 aggccgaacg tcttgtcgag gaatgcccac aggaagaccc agccgaggct cagccgcacg
     2281 gccacggaaa cgtaccgggc ggtcttcatc gagcgggtct ccggagccac gacctcggga
     2341 tgtacgaagt ccggatccgg gaggcgtagg tccttcttca tctggttgct catgacaccc
     2401 tccctcggag tgctggttga tttcgtcgtc acttccactc tggtgaccgc ggccggcgta
     2461 caggagaggc ggaggccatc gactgtcagg gactttcgtc cttcagcagc gccccggatc
     2521 gaggtggcaa tgacagcgag gcggcaagga cgtggcgaca cgccggcaaa ctccaactat
     2581 ccctaggggt cccaggtagg cccatgctct gctgcgcgtg cccgaggcgc gatctgcggc
     2641 gttggagtcg tcgcccgatg ctgggcatcg atctcctcct ccgcctggca gctcatccac
     2701 ctcgaccccg ctcgcgacga gcgggaccca cctgacaccc ctgccacctc ggtgggagag
     2761 cgcgagggag ggatcagatg ccgaggtgct tggcggcgaa gacggcggcc tgggtacgtc
     2821 gctccatgcc cagcttggcg agcaggctgg acacatagtt cttgacggtc ttctcggcaa
     2881 ggaacatcgt ctcggcgatc tgccggttcg tcatgccttc gccgatgtgc tgcaggatgc
     2941 gccgctcctg tgcggtgagt ggcgcgagtg ccttgtcctc ctcgggtccc ttccggagcc
     3001 ggtcgagtac ctgttgcgtc accgccggat cgagcatgga ctggccgtcg gccactcggc
     3061 gtacggtgtc gacgaggtcg ttgccgcgga tctgcttgag cacgtatccc gccgcgcccg
     3121 ccatgatcgc gctgaacagc gcctcgtcgt cgtcgaacga cgtgaggatc agcgcggcga
     3181 tctccgggtg acgtgaccgg atctcccggc acacgtcgat gccggacccg tcgggcagtc
     3241 tgccgtcgag cacagccacg tcgggacgca gcgccgggat ccggcgggtt gcctcctcgg
     3301 caagtccgga ctcaccgaca acttcgatgt caccatcctg ctcgagcagc tcgcgcaggc
     3361 cgcgacgaac gacctcgtgg tcgtccagga ggaacactcg gatcttctcg gcgcgcgcag
     3421 acgtgtcggt cataccccca cggtagggcc gcgggacacc gcagacgagg gacgaatgtg
     3481 gtctcgcggc gggactttgg tccctcgcaa ccgacccgtc aggacaccgg aacacgccag
     3541 cacaggcagc tgccggctcc tggtgtcgac tcgatgccga gctcgccacc gagatcctcg
     3601 gcccggtcgc gcatgttcgc caggccgtgg ccaccttcgt ccccgccact gacgtcgagg
     3661 cccacgccgt cgtcgcggac aacgagcgtc agggcggcgt ccccgacctc gatctcgacc
     3721 gtgacctgcg acgcccgcgc gtgccgggcg acgttcgaaa gggcctcccc cagcaccgcc
     3781 aggagatgag gtgcgatctg atcgggtacg ccgtgcgcca ccgggccggt ggtccgcaga
     3841 gctggcgtga acccgaggct ctgcgccgac cgggtcacga catcgtcgat gtcgtgccgc
     3901 aggtcgcgga cctgttccgg ggcgctgaga tggaagatcg tgcggcggat gtccttgatc
     3961 gtcgagtcga tgtcgtcgac ggccgtcacg agtcgctcgg ccacctcggg cttggccgtg
     4021 atccgtaccg tgctctccag gctgaggccg accgcgaaca gctgctggat gacgaggtca
     4081 tgcaggtcgc gcgcaatccg gtcccggtct tcgagcaccg ccagccgttg ctgcgtctcc
     4141 cgcgaccgtg cgacctggat cgccagtgcc gcctgctcgg cgtaccgctc gatgaccggt
     4201 acgtcgatgt cgcgcagctg gctcgtgtgc tccttgaacc accccatgct cagggcaccc
     4261 tcgtcgccgt cgggagtccg catcggcacc acgagcaccg caccgaggct gagcaggtcc
     4321 tccgacgacg ggagctcgtt cccgatccga gggtctgcgg cgacgtcgtc cacgacgagt
     4381 gtcctgccgg tctccagcac aaccgccgac agcgtccggt cccgggccag cgccatcccc
     4441 ggcacgatgt gtggcagctc gccggcaaca gcccggacga ccagcttccc gtcctcgtcg
     4501 gtgacgacga ccatcgcatg atcggcggcc gactgttccg ccgcccgtcg ggcgaccagg
     4561 tcgaatgcca cctgcggttc ccccggcccg agcagcgcgg cgacgacctc ggcggcggct
     4621 tgcagccagg tctcccgccg ggcggcctcg ccgtacagcc gtgcattgtc gatcgcgacc
     4681 cccgccgcgg cggccagcgc gagcacgaca tcctcgtccg cctcgtcgaa gtcctcacca
     4741 ctcgccttct ccgacaggta gaggttgccg aacacccggc cctggacccg gatcgggacc
     4801 ccgaggaacg acctcatcac cggatggtcc ggggggaagc cgaacgacgc cggatgcgtc
     4861 gtgatgtcgt gcaggcggag cggctccgga tggtcgatga gcaggccgag cacgccgagg
     4921 ccgtgcggga ggtctccgat gagtctgcgc tgctcgtcat cgatgcccac gtacacgaac
     4981 tcacgcagcc gcttgtcacg ctcggagccg atcacaccga gtgcggcgta cctggcaccc
     5041 gtcagttgcc gggccgcctc gatgatgcgt tgcagtacgc cgtccagcga caggtcggag
     5101 ccgatgccca ccacggcgtc gagaagcggg ctgtgcggcc gacggtccgt cccgtccgcc
     5161 gcctcgtggc cccgactcgt acccacgtgc cacctctcgt tctcgcgggc tcagcctagg
     5221 acgggacctt cgacctatgc agtcggcacc gtcgaccctc cccagacaac cgtcggctgt
     5281 cgcacggtcg aagacgagaa gtgcgacggt tgcgtccgct acgacgcctc cgtcgaccgc
     5341 gacgaaggag gctgtcatga caacccagac ccaacccacc gacgagtcca gcacctccgt
     5401 cccggtcggc gtcgtcattg tcggcgtcga cggttcggac aagaaccagg ctgccttgga
     5461 gtgggccgac cgcgaggccg accgactcgg ctaccggctg gtgctcgtgg gcgcaacgcc
     5521 gatcttcggg acacccacct ggggcttcac gcgtgactac gggttcgccg agctcgagca
     5581 ggacatgcga gaccagctcg acgccacccg ggcacgactg gagcggccag gtcgagacat
     5641 ccgcgtcgac gcggcaaccg gtgacgcgac acgcgtcctc ctcgaggccg gcaagcactg
     5701 cgacctgctc atcgtcggca agcgtggtat cggggccacc aaacggatgc tggtgggcag
     5761 tacgtcgacc gcggtcgcag ggcggagccc ggttccggtc gtggtcgttc cggacagctg
     5821 ggaccagtcc cggcaggagt ccgcacccat cgtcgtcggt gtcaccgacg ccgaacgcga
     5881 ccacgaggtc ctcgagttcg ggttcacccg cgccgatcgg ctcggcgtcc cactgatcgc
     5941 ggcatacgtg tgggacctgc cgttgcaggt cgtctggtcc ccggacgacg tggcacggtg
     6001 gaaagccgag gcacgcgaac tgttggacgg cgtcgtggca cagtggtcca agcggttccc
     6061 cgacgtcgag gtggtgtcgc tcaccccgca cggcaacgtc gcgatggcgc tcgtcgacgc
     6121 tggtgaggca gcgcagctcg tggtcgtcgg ccgccacacc ggtccgcggc acctcggtgg
     6181 attctcgctc ggctcgaaga cacgggcgtt gctgcactac gcggagttcc ccgtggccgt
     6241 ggtgccgacc aagcacgtca agtccgaccc gttggccgat cttggttgat gatggagga
//
LOCUS       DATBFZ010000033         2839 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_1005188, whole genome shotgun sequence.
ACCESSION   DATBFZ010000033 DATBFZ010000000
VERSION     DATBFZ010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2839
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1005188"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            163..1539
                     /locus_tag="VH419_01015"
     CDS             163..1539
                     /locus_tag="VH419_01015"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HEY7042225.1"
                     /translation="MDLPEREGRAEAAEQLVSQLQDQGVVAVATTFVDNSGIARVKCV
                     PLDRFPKLAAWGVGSSVAFDRFRFDDWIAADSDGRAPVGDLRIIPDVRRVVALSAQPG
                     WAWAPADRYDQEGHPHDQCSRLLLRRLVERLADRSLALTTAIEIEWVVSDEPGDEFHP
                     GVRGPAYGMARLIDASDYALDLITSLTEEGVEVEQFHPEYAPGQLELSVRAESPLDAA
                     DTSVLVRSTIRAVGAACGLRTSYSPKVAVPGVGNGGHVHFSLWREETNLMIGGDGPFG
                     LTREAAAFSAGVLAHLPGLMAVGAPSVVSYLRHVPSHWAGTFACWGLENREAAMRMVT
                     GSTGSSHWAANLETKCFDLHANPYLLLAGIIAAGLDGLTRDAVLPEPVDVDPAVYDDG
                     ELGRRGISRLPQDLRTATDAFATDDVLRDAFGPALTASILAVRESEIELFDGVSDEEL
                     AAATRWQH"
     gene            complement(1670..2167)
                     /locus_tag="VH419_01020"
     CDS             complement(1670..2167)
                     /locus_tag="VH419_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009073768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4396 domain-containing protein"
                     /protein_id="HEY7042226.1"
                     /translation="MTEHRHHGHADTGVMEHGADASWRTATTATLHCLTGCAIGEVLG
                     LVLATWWGWSNGPSIALAVVLAFVFGYSLTMGPVLRAGVGFKKAVGIALAADTVSITT
                     MEIVDNVFVLLVPGAMAAGLLDGLFWGSLVVSLIIAFVVTVPVNRAMIARGKGHAVVH
                     AYHHH"
     gene            complement(2227..>2839)
                     /locus_tag="VH419_01025"
     CDS             complement(2227..>2839)
                     /locus_tag="VH419_01025"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HEY7042227.1"
                     /translation="TDAAADVARFQVVLTARSSHCDPAAPPDLHLFPAGPFPAEGVPG
                     TAVFGIVTALLAPRARGSVRIRSADPNEPPSIRTPLLDHGEDVARMVDATLLARTLSR
                     TPPLAGFVSADELEPGSAILDDDHEGLARSIRGRVASYHHPVGTCAMGPDPDGGAVVD
                     ATGAVYGVEGLWVADASVMPTIPSAGTNMSTILVATKIAEGLR"
BASE COUNT          449 a          996 c          971 g          423 t
ORIGIN      
        1 caggcgttcg aactctgccc ggctcaggtc gcggcgcacg agcccgtggc agtggtgatc
       61 gaccaacggt ccatcgacga aacgcgtcac agcgtgccct agtctgagcg tgacctgcgg
      121 gtttgtccat cacgtgtgca ccaggacgac gggaggagca ccgtggacct gccggagcgc
      181 gaggggcgcg ccgaggccgc cgagcagctg gtcagccagc tccaggacca gggcgtcgtc
      241 gcggtcgcga ccacgttcgt ggacaactcc gggatcgcgc gggtcaagtg cgtccccctc
      301 gaccggttcc cgaagctggc cgcctggggc gtggggagct cggttgcgtt cgaccggttc
      361 cgcttcgacg actggatcgc cgccgactcc gacggtcggg caccggtcgg cgacctgcgc
      421 atcatccccg acgtccgccg cgtcgtcgcg ctgtcagccc aaccggggtg ggcgtgggca
      481 ccggcagacc gctacgacca ggagggtcac ccgcacgacc agtgcagccg gttgctgctg
      541 cgccggctgg tcgagcggct tgccgaccgg tcgctcgcgc tcaccacggc gatcgagatc
      601 gagtgggtcg tcagcgatga gcccggcgac gagttccatc ccggggtacg cggtccggca
      661 tacggcatgg cgcggttgat cgacgcgtcg gactacgcgc tggacctgat cacgtcgttg
      721 accgaagaag gcgtcgaggt cgagcagttc catccggagt acgcacccgg acagctcgag
      781 ctctcggtgc gagccgagtc gccactcgac gccgccgaca cgagcgtgct ggtccgctcg
      841 accatccgcg ccgtcggtgc ggcgtgcggg ctgcggacgt cgtactcgcc caaggtcgct
      901 gtccctggcg tgggcaacgg cgggcacgtg cacttcagcc tgtggcgcga agagaccaac
      961 ctgatgattg ggggcgacgg tccgttcggc ctgactcgag aggcggcggc cttctccgcc
     1021 ggtgtcctgg cgcacctgcc gggtctgatg gcggtcggtg cgccgagtgt cgtcagctac
     1081 ctgcggcacg tgccgtcgca ctgggccggc acgttcgcct gctggggtct ggagaaccgc
     1141 gaggcggcga tgcggatggt gacgggctcg acgggcagca gccactgggc cgccaacctc
     1201 gagaccaagt gcttcgacct gcacgccaac ccgtacctcc tcctcgcggg gatcatcgcc
     1261 gccggcctcg acggcctgac acgcgatgcc gtgctgccgg aaccggtgga cgtcgaccca
     1321 gcggtgtacg acgacggcga gctcggccgg cgcgggatct cccgacttcc gcaggacctg
     1381 cgtacggcga ctgacgcctt cgccactgac gacgtactcc gcgacgcgtt cggccccgcc
     1441 ctgaccgcct cgatcctcgc cgtacgggaa agcgagatcg agctcttcga cggtgtcagt
     1501 gacgaggagc tggcagcggc aactcgctgg caacactgat tccgaggaag cggccccctg
     1561 accactgcgt cgggttgccg cgaattgcgg gaaaggtcat cgcaaaacca ccttcgaagc
     1621 atcacgtttc ccgcaattcg caggagctcc ggagcgcgga ggggggagct cagtggtggt
     1681 ggtaggcgtg gacgaccgcg tggcccttcc cgcgtgcgat catcgcccgg ttgaccggca
     1741 cggtgacgac gaacgcgatg atgagtgaga cgaccagcga tccccagaac agcccgtcga
     1801 gcagacctgc ggccatcgcg ccgggcacca acaacacgaa cacgttgtcg acgatctcca
     1861 tcgtcgtgat cgagaccgtg tcggcggcca gcgcgatccc gaccgccttc ttgaaaccga
     1921 cgccggcgcg cagtaccgga cccatcgtga gggagtagcc gaagacgaac gccagtacga
     1981 cggcgagcgc gatgctcggc ccgttcgacc agccccacca cgtcgccagc accaggccga
     2041 gcacctcacc gattgcacaa ccggtgaggc agtgcagggt tgccgtcgtt gcggtccgcc
     2101 acgaggcatc cgccccatgc tccatcacac cggtgtccgc gtgcccgtga tgacggtgct
     2161 cggtcatgac ctcacgatat accaccccag ggtatgtgac gccactcggc tgccggactg
     2221 ccgtggttat cgcaagcctt cggcgatttt cgtggccacg aggatggtgc tcatgttcgt
     2281 gccggccgac gggatcgtcg gcatcaccga cgcgtcagcg acccagagac cctcgacgcc
     2341 gtacaccgca ccggtcgcgt cgaccaccgc gccaccgtcc gggtccggcc ccattgcgca
     2401 agtgccgacc gggtggtggt acgaggcaac ccgtccccgg atcgacctcg cgagcccttc
     2461 gtggtcgtcg tccaggatcg cggatcccgg ttccagttcg tcggccgaca cgaacccggc
     2521 aagcggcggc gtacgactga gcgtgcgtgc caacagcgtg gcgtcgacca tccgcgcgac
     2581 gtcctcgccg tgatcgagca gcggtgtacg gatcgacggc ggctcgttcg ggtcggcgga
     2641 ccggatccgc accgaccccc tggcgcgcgg cgccagcagc gcggtgacga tgccgaacac
     2701 cgccgtaccc ggcacgccct ccgccgggaa cggtcccgcc gggaacaggt gcaggtcggg
     2761 tggcgccgcc ggatcgcagt ggctcgaccg cgcggtcagc accacctgga accgcgccac
     2821 gtccgctgcc gcatcggtc
//
LOCUS       DATBFZ010000034         2948 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_352101, whole genome shotgun sequence.
ACCESSION   DATBFZ010000034 DATBFZ010000000
VERSION     DATBFZ010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352101"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(31..1194)
                     /locus_tag="VH419_01030"
     CDS             complement(31..1194)
                     /locus_tag="VH419_01030"
                     /inference="COORDINATES: protein motif:HMM:TIGR01187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY7042228.1"
                     /translation="MSGGHVKLESLTKRFDGVTAVDGISLEIQAGEFFSLLGPSGCGK
                     TTTLRMVAGFEPPSGGQILLDGTDVGTLAPHRRNVNTVFQSYALFPFLTVAENVAFGM
                     RYKSVPKSERDSRVAEALALVQLSGFEKRRPNQLSGGQQQRVALARALVLRPSVLLLD
                     EPLGALDAKLRRTLQVELTALQKQVGITFLYVTHDQEEALTMSDRLAVMNHGRIAQLG
                     TPEEVYNEPADAYVADFLGVSNLMDARVEPRGNGQGPCRLRVGDFTLEAKAGQLDATG
                     AVKVAIRPERIHLRPYEAAGVNCVPGMVERLVFLGSTTHIYVRLATGSALQALVRNEG
                     SAVPYNQGTPVKVDLPADALRVLPDPGVSVAEIHDETLGDDPLDLTSAAPTSS"
     gene            complement(1216..2154)
                     /locus_tag="VH419_01035"
     CDS             complement(1216..2154)
                     /locus_tag="VH419_01035"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY7042229.1"
                     /translation="MASTIREPVTTSSEDRVKPTSPGRRRGNPWRRPWAMEGFTWLYI
                     VWSIVPIAIAVLFSFNKGASQATWQGFSTKWYVGDQVTSVLHNPSLHQAVVQTIVLAV
                     LTTLICVPIGVCFAIGIDRWRTRTSKGLNIVMIFAFVIPELLLAVALFLLVTQLLTFI
                     KLGTPAEVAGLVVWNISWPAIIVRARLVSIGRSYEEAACDLGASTLGAIRRVLLPLLM
                     PAIFASSVLVFASTVDDFVIVEQLSSTANTQPMSVLIYSNVHAGLSGPALNALATIML
                     VLSLLAALIGYVGYRWMSRGERTKGGADALTGIAGI"
     gene            complement(2154..>2948)
                     /locus_tag="VH419_01040"
     CDS             complement(2154..>2948)
                     /locus_tag="VH419_01040"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY7042230.1"
                     /translation="VAGGGVNEFFQTVVPVWNPLNWSSWNLSAVWHEIFGTGSFIGPP
                     ALRTLVYTAIATVISLAVSYPVAYFVSRYAGRRKTMFLVLLIAPFWVSYMMRMLAWID
                     LLQQDGFFNSALSFFGLPGNTDWLGGKSVTVILGLVYGYIPYVILVLYAFLDRIDQSM
                     LEASRDLGLSKWRTFWHVTIPLSKPALIAAAFITVLPMLGDYYTNQLLSGAPGTTMIG
                     NVIEGQLNAPGEIGQGAVLSLMLLVVLLIPMIYYVVSTARSSMENA"
BASE COUNT          539 a          973 c          953 g          483 t
ORIGIN      
        1 ccctcggcgc cgttcggctg gggtccgtgc tcagctcgag gtcggcgccg cgctggtcag
       61 gtcgagcggg tcgtcgccga gcgtctcgtc gtgaatctca gcgacgctga cgcccgggtc
      121 cggaaggacg cgcagcgcat cggccggaag gtccaccttg acgggcgtcc cctggttgta
      181 aggcaccgcg ctcccctcgt tgcgcaccag cgcctggagg gctgagccgg tggcgaggcg
      241 gacgtagatg tgcgtggtcg agccgaggaa cacgagacgc tcgaccatcc ccggcacaca
      301 gttgacgccg gctgcctcgt aaggacgcag gtggatgcgc tccggtcgga tcgcgacctt
      361 gacggcgccg gtcgcgtcga gctggcctgc cttcgcctcc agagtgaaat cgcccacacg
      421 caggcggcag ggtccctgcc cattgccgcg cggctcaact cgagcgtcca tcaggttcga
      481 cacccccagg aagtcggcca cgtaggcatc ggcgggctcg ttgtagacct cttccggtgt
      541 gccgagctgg gcgattcgtc cgtggttcat gacggccagg cgatcggaca tcgtgagcgc
      601 ctcttcctgg tcatgggtga catacaggaa tgtgatcccc acctgcttct ggagcgcggt
      661 caactccacc tggagcgtcc ggcggagctt tgcgtccagt gcccccaggg gctcgtcgag
      721 cagcaggacc gacgggcgca gcaccagtgc ccgtgccagc gcgacgcgct gttgctggcc
      781 gcccgaaagc tggttggggc gtcgcttctc gaaacctgac agctggacaa gcgcgagcgc
      841 ttccgcgacc cggctgtcgc gctcggactt cggcaccgac ttgtacctca tgccaaaggc
      901 gacgttctcg gcgacggtga ggaatgggaa cagcgcatag ctctggaaca ccgtgttgac
      961 gttgcggcgg tggggcgcga gggttccgac gtcggtgccg tcgagcagga tctgtcctcc
     1021 gctcggcggc tcgaagcccg cgaccatgcg cagcgtggtc gtcttgccac acccgctcgg
     1081 cccgagcagc gagaagaact cgccggcctg gatctcgagc gagatcccgt cgaccgcggt
     1141 gacaccgtcg aaccgctttg tcagtgactc gagcttgaca tgtcctccac tcatttcagt
     1201 cctctcaccg tcctctcaga ttcccgcgat gccggtcaaa gcgtcggccc cgcccttggt
     1261 gcgctcaccc cggctcatcc acctgtagcc gacgtatccg atcagcgcgg cgaggagcga
     1321 caggacgagc atgatcgtgg ccagcgcgtt gagcgccggg ccactcagtc ccgcatgaac
     1381 gtttgagtag atcagcaccg acatcggctg cgtgttcgcg gtcgatgaca gctgctccac
     1441 gatcacgaaa tcatcgacgg tgctcgcgaa gacgagcacc gagctggcga agatcgccgg
     1501 catcagcagc ggaagcagca ctcgccggat cgccccgagc gtggacgccc caaggtcgca
     1561 cgccgcctct tcgtagctcc gcccgatcga caccagccgc gctcgcacga tgatcgcggg
     1621 ccagctgatg ttccacacca cgagtccggc aacctcggcc ggcgtcccga gcttgatgaa
     1681 cgtcagcagt tgggtgacga gcaggaacag cgcgaccgcg agcaacagct ccgggatcac
     1741 gaacgcgaag atcatcacga tgttcagtcc cttcgacgtc cgggtccgcc agcgatcgat
     1801 gccgatcgcg aagcagacgc cgatcggaac gcagatcagc gtggtgagaa ccgcgagcac
     1861 gatcgtctgg actaccgcct gatgcaggct ggggttatgg aggaccgagg tgacctggtc
     1921 gccgacgtac cacttggtcg agaagccctg ccacgttgcc tgcgatgcgc ccttgttgaa
     1981 cgagaacagc acggcgatcg cgatcggcac gatcgaccac acgatgtaaa gccatgtgaa
     2041 gccctccatc gcccacggcc ggcgccacgg gttaccgcgg cggcgcccgg gcgacgtggg
     2101 tttcacccgg tcttcggacg aggtcgttac aggctcacgg atcgtgctgg ccatcaggcg
     2161 ttctccatcg aggaacgggc ggtactgacc acgtaataga tcatcgggat cagcagcacg
     2221 accagcagca tcagcgacag caccgcaccc tgtccgatct cgccgggcgc gttgagctgc
     2281 ccctcgatca cgttgccgat catcgtcgtc ccgggtgccc ccgacagcag ctggttggtg
     2341 tagtagtcac cgagcatcgg cagcacggtt atgaacgctg cggcgatgag cgcgggcttg
     2401 ctcaatggaa tcgtcacgtg ccagaaggtc cgccacttgc tgagtccgag atccctggag
     2461 gcctccagca tgctctggtc gatgcggtcg aggaacgcgt agagcaccag gatcacgtac
     2521 gggatgtagc cgtacacgag ccccaggatc acggtcaccg atttgccgcc gagccagtcg
     2581 gtgttgccgg gcaggccgaa gaaggacaac gcgctgttga agaagccgtc ctgctggagg
     2641 aggtcgatcc acgccagcat ccgcatcatg tagctgaccc agaacggcgc gatcagcagt
     2701 accaggaaca tcgtcttacg ccttccggcg tagcgcgaga cgaagtacgc caccgggtac
     2761 gagaccgcaa gcgaaatcac ggtcgcgatc gcggtgtaga cgagcgtccg cagcgcaggg
     2821 ggaccgatga acgatccggt cccgaagatc tcgtgccaga ccgcgctcag gttccagctc
     2881 gaccaattca gcggattcca gacggggacc accgtctgga agaactcgtt gacgccaccg
     2941 ccggccac
//
LOCUS       DATBFZ010000035         5388 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_352892, whole genome shotgun sequence.
ACCESSION   DATBFZ010000035 DATBFZ010000000
VERSION     DATBFZ010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 5388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5388
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352892"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="VH419_01045"
     CDS             complement(<1..363)
                     /locus_tag="VH419_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY7042231.1"
                     /translation="MLTPYRKVLSVPGALAFTSAGFLARLPISMATLGIVLLVVGETG
                     SYGQAGAVSAAFIIGEGCAAPLLARVIDRFGQGEIVLPAALVYGTGLVLLTVSVRADA
                     PKPVTLVCALVTGASYPPI"
     gene            431..1588
                     /locus_tag="VH419_01050"
     CDS             431..1588
                     /locus_tag="VH419_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020575692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEY7042232.1"
                     /translation="MSHPRDVEDGELATGVSTSSTTGLPRDAAPYDALLLVSFGGPEK
                     PGDVMPFLENVTRGRGVPRERLEQVAQHYFDLGGRSPINDQCRELVAAIEADFKECGL
                     DVPVYWGNRNWEPYLSDTVATMRGDGVRRAVCFVTSAYASYSGCRQYRENLYEAVADV
                     ADAPRLDKLRHYFNHPGFVEAFVDSTSAGLANVPAQHRQHAHLVFVTHSIPVAMADTA
                     GPDGGAYVAQHLSVASLVSTAVQQQTGVKHPWELVYCSRSGPAQVPWLEPDVNDHLRR
                     LASDGVRAVVIVPLGFVSDHMEVVYDLDTEAAQTAGDLGLEFVRAATPGVHPAFVATV
                     RDLLLERAARERGERVVPAAFGGLPAAWDVCRAGCCPNPRASRPALCETPA"
     gene            1585..2421
                     /locus_tag="VH419_01055"
     CDS             1585..2421
                     /locus_tag="VH419_01055"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012921381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEY7042233.1"
                     /translation="MTDDRALLDLAVSTARQAGELVQRGRQGDVHVTATKSSPTDIVT
                     DSDIASERLIRSAILAARPDDGFFGEEGDYDEKSAGTSGVTWVADPIDGTVNYLYDIP
                     QYAVSIASQRAGRTVVAVVHNPTSGETFTATAGGGAWLDGSPISVSGVTEVAAALVGT
                     GFHYRSDVRAHQAAETAALLPSVRDIRRLGSAALDLCFLACGRLDAYVERGLRPWDLA
                     AAQLVVSEAGGRVEGMFGSAPGELVAVAAGTGLFDAFHERLVDCGYADWPMPDWPPPR
                     TT"
     gene            2716..3015
                     /locus_tag="VH419_01060"
     CDS             2716..3015
                     /locus_tag="VH419_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012921380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4193 domain-containing protein"
                     /protein_id="HEY7042234.1"
                     /translation="MATDYDAPRKTDEELGEDSLEELKARRHDKNSGKVDEDEVEAAE
                     SFELPGADLSHEELSVQVLPRQEDEFTCSSCFLVHHRSQLASEKNGVLICTDCAA"
     gene            complement(3086..3433)
                     /locus_tag="VH419_01065"
     CDS             complement(3086..3433)
                     /locus_tag="VH419_01065"
                     /inference="COORDINATES: protein motif:HMM:NF025385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4235 domain-containing protein"
                     /protein_id="HEY7042235.1"
                     /translation="MADTSKKSDRPKPKGKRSWKLLGSTAALVAGLATAKALDAAWRT
                     ATGKQPPDAPESPEIGNVEALAWAAASGLAMGVARMYATRKAANYWVKSFGTLPPGMD
                     KGATKETKKKVAR"
     gene            complement(3446..3913)
                     /locus_tag="VH419_01070"
     CDS             complement(3446..3913)
                     /locus_tag="VH419_01070"
                     /inference="COORDINATES: protein motif:HMM:NF022736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3093 domain-containing protein"
                     /protein_id="HEY7042236.1"
                     /translation="MASPPRSLAYSERLHVPPGWWLLGLAFVLAVGWVFFVATPHIVT
                     LVAVAVTAAVVVVAVQRYGELQVQVDDQTLRAGRGVLPLENVGRVQAFDAASTRRQLG
                     PDADARAYLVTRPYCATSVRVEVVDDRDPTPYWLISTRRPEELAASLRGSRGE"
     gene            4030..4497
                     /gene="dut"
                     /locus_tag="VH419_01075"
     CDS             4030..4497
                     /gene="dut"
                     /locus_tag="VH419_01075"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020658000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEY7042237.1"
                     /translation="MLEVLVQRRDGVPLPTYAHPGDAGADLVSTVDITLQPGERALVP
                     TGVSIAMPDGYVGLVHPRSGLAARCGLSIVNAPGTIDAGYRGEVKVVLVNLDPHEPVT
                     LSRGDRIAQLVFQQVERARFVEVDRLPGSHRGDGGYGSTGGMAAHRPVQEDVS"
     gene            4494..5195
                     /locus_tag="VH419_01080"
     CDS             4494..5195
                     /locus_tag="VH419_01080"
                     /inference="COORDINATES: protein motif:HMM:NF023917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3710 domain-containing protein"
                     /protein_id="HEY7042238.1"
                     /translation="MIFRRRKSRPDADDASELVESPDEGQPEELDELDELSRARPHGP
                     WDRSETKADEDDPAYVDLGGLVVRGREGLELRLQVNEATQLVEAVMLAGAESGLELRA
                     FAAPRSASIWDDVRRDIAAEASKRGGTATEVDGEFGTELKVVLPVQTAQGRQATQPTR
                     IVGVEGPRWLLRGTFLGASAQQADPDGVVENAFRDVIVVRGDGPMAPRDMIAMRMPRG
                     AEVTATSEPEPTDEL"
BASE COUNT          853 a         1839 c         1884 g          812 t
ORIGIN      
        1 gatcggtggg tagctcgcac cggtgacgag ggcgcagacc agcgtcaccg gcttgggcgc
       61 gtcagcccgc accgagaccg tcaggaggac gagtccggtg ccgtacacga gcgcggcggg
      121 gagcacgatc tctccctgcc cgaaccggtc gatcacccgg gccagcagcg gtgcggcgca
      181 gccttcgccg atgatgaagg ccgctgacac ggcgccggcc tggccgtacg agccggtctc
      241 gccgaccacc agcaacacga tgccgagcgt cgccatggag atcggcagcc gggccaggaa
      301 ccccgccgac gtgaaggcga gtgccccggg cacggacaac accttccggt acggcgtgag
      361 catgaccctc accctatgag tccacgggga cgcgccgggc cggtcgtggc cgggtggcag
      421 gatcgtcccc atgtcccacc cccgtgacgt cgaggacggc gagctggcca ccggggtctc
      481 gaccagctcg acgactggac tgccgcgaga cgcggcaccg tatgacgcct tgctcctggt
      541 gtcgttcggt ggaccggaga agccgggcga cgtgatgccg tttctcgaga acgtgacgcg
      601 tggccgcggc gtcccgcggg aacgcctcga gcaggtcgca cagcactact tcgacctcgg
      661 tggtcgcagt ccgatcaacg accagtgtcg cgagctggtg gcggcgatcg aggcggactt
      721 caaggagtgc ggcctcgacg tgccggtgta ctggggcaac cgcaactggg agccgtacct
      781 gtccgacacg gtcgcgacga tgcggggcga cggcgtacgc cgggcggtct gtttcgtgac
      841 gagcgcctat gcgtcgtact ccggctgtcg ccagtaccgc gagaacctct acgaggccgt
      901 ggcggacgtg gccgacgcgc cacggctgga caagctccgg cactacttca accacccagg
      961 cttcgtcgag gccttcgtgg acagcacgtc ggccgggctg gcgaatgtgc ccgcgcagca
     1021 tcggcagcac gcgcacctgg tcttcgtcac gcactcgatc ccggtggcca tggcggacac
     1081 ggctggtccc gacggtggcg cctacgtggc gcagcacctg tcggtcgcca gcctggtgtc
     1141 aacggccgtg caacaacaga caggcgtgaa gcatccgtgg gagctggtct actgcagccg
     1201 atccggacct gcgcaggtgc cttggctcga acccgacgtc aacgaccacc ttcgccgact
     1261 cgcctccgat ggggtgcgcg cagtcgtcat cgtcccgctc gggttcgtgt ccgaccacat
     1321 ggaggtcgtc tacgacctcg acaccgaggc ggctcagacc gccggtgacc tcggcctcga
     1381 gttcgtccgg gcagcgacgc cgggtgtcca tccggcgttc gtggcgaccg tgcgtgacct
     1441 gctgctcgag cgtgccgccc gtgagcgggg tgaacgcgtc gtacccgccg ctttcggtgg
     1501 gcttccggcg gcgtgggacg tgtgccgggc aggctgctgc cccaacccgc gcgcgagccg
     1561 gccggcgctg tgcgagacgc cggcatgacc gacgatcgcg ccctgctgga cctggcggtg
     1621 tccaccgctc gacaggccgg cgagctcgtc caacggggtc gacagggcga cgtccacgtc
     1681 accgccacca agtcgagccc gaccgacatc gtcaccgaca gcgacatcgc gtcggagcgg
     1741 ctcatccggt cggcgatcct ggcggccagg cccgacgacg ggttcttcgg cgaggagggc
     1801 gactacgacg agaagtccgc cggaacctct ggtgtcacgt gggtggccga cccgatcgac
     1861 ggcaccgtca actacctgta cgacatcccg cagtacgccg tgtcgatcgc ctcgcagcgt
     1921 gccggccgga ccgtcgtcgc cgtcgtgcac aacccgacgt ccggcgagac gttcaccgcc
     1981 accgcgggtg ggggagcctg gctcgacggg agcccgatat cggtttccgg tgtcaccgag
     2041 gtcgcggcag cactcgtcgg caccggcttc cactaccgct ctgacgtgcg tgcccatcag
     2101 gctgcggaaa cggcggcgtt gctgccgtcg gtgcgagaca tccggcggct gggttcggct
     2161 gcccttgacc tgtgtttcct cgcgtgcggg cgactcgacg cgtatgtcga gcgggggctg
     2221 cgaccctggg acctggcggc cgctcagctc gtcgtcagcg aagccggcgg acgggtcgag
     2281 ggcatgttcg gctccgcgcc cggcgagctc gtcgcggtcg ccgcgggcac cgggctgttc
     2341 gacgcgttcc acgagcgcct ggtcgactgc ggctacgccg actggccgat gccggactgg
     2401 ccgccgccgc ggacgacctg actcgtctac gacatcggcc tggccaggac cacgtcccga
     2461 aaccccgttg tcacctggca tttcgccatc gttcaccaac agaatccggt gcgaaaagta
     2521 tgggtttggc cgcgttgagt aggactccgg acggtgactg ggcgccatga gatgagacac
     2581 acacgaattg acgtggcgcg agagcgcgaa atcgggcaca atctcgccgc cagagcgggc
     2641 acaaatcgcg aacgtgctgc gttacacgca gagcaccgca gacgatcgac ggacgagggg
     2701 aactgagcac acgccatggc taccgactac gacgcaccac gtaagaccga cgaagagctg
     2761 ggcgaggact ctctggagga gctcaaggct cgtcgtcacg acaagaactc cgggaaggtc
     2821 gacgaagacg aggtcgaggc cgcggagtcc ttcgagctgc ccggcgcgga cctgtcccac
     2881 gaggaactct ccgttcaagt gctgcctcgg caggaggacg agttcacctg ttccagctgt
     2941 ttcctcgtcc atcaccgcag tcagctcgcc agtgagaaga acggcgtact catctgcaca
     3001 gactgcgctg cctgaccgtc cactgagctg tcaggtgccg gcccttccag cgcctctcga
     3061 cgacgcccgc tgaccattca tcgcctcagc gggcgacctt cttcttggtc tccttcgtcg
     3121 cacccttgtc catccccggt ggcaaggtgc cgaaggactt cacccagtag ttcgccgcct
     3181 tgcgggtcgc gtacatcctc gccaccccca tggcgagacc gctggctgcg gcccaggcga
     3241 gcgcctcgac gttcccgatc tccgggctct ccggagcgtc cggcggctgc ttgccggtgg
     3301 cagttcgcca ggcggcatcc agcgccttgg ccgttgcgag gccggcgacc agggcagcgg
     3361 tcgaacccag cagcttccag ctccgcttgc ctttgggctt gggacggtca gacttcttcg
     3421 acgtgtcggc cacgaggcca gtctgtcact cacctcgtga accgcgcagg ctcgcggcca
     3481 gctcttcggg gcgtcgggtc gagatgagcc agtacggcgt cgggtcccgg tcgtcgacca
     3541 cctcgacccg gacggacgtg gcgcaatacg gccgcgtcac gaggtacgcg cgggcgtcgg
     3601 catcaggacc gagctgtctt ctcgtcgatg cggcgtcgaa cgcctggacc cggccgacgt
     3661 tctcgagcgg caggacaccc cggccggccc gcaacgtctg gtcgtccacc tggacctgca
     3721 gctcgccgta tctctgcacc gccacgacga cgacggcggc cgtcaccgcg accgcgacca
     3781 gcgtgacgat gtgcggcgtc gccacgaaga acacccagcc gaccgccagc acgaacgcga
     3841 gtccgagcag ccaccagccc ggcggcacgt gaagtcgttc gctgtacgcg agcgaacggg
     3901 gtggtgaggc cacccggtca gcctcccacg gtgcgcacgc cccaggttgc gcgagtaggc
     3961 tgaccagccg attccgcacg tggccgtgac cggcgacacc agcacgcagg catcagcgag
     4021 gaggcccagg tgctcgaggt tctcgtccag cgtcgtgacg gcgttccgct gccgacgtac
     4081 gcgcatcccg gcgacgccgg tgctgacctc gtctcgacgg tggacatcac cctgcagccc
     4141 ggtgagcgcg ccctggtgcc gaccggcgtg agcattgcca tgccggacgg ctacgtgggt
     4201 ctggtgcacc cgcgatcggg gctcgccgcc cgctgcgggc tctcgatcgt gaatgcgccc
     4261 ggcacgatcg atgctgggta tcgtggcgag gtgaaggtcg tgctcgtcaa cctcgaccca
     4321 catgaacccg tgacgttgtc gcgaggggac cggatcgccc agctcgtctt ccaacaggtc
     4381 gagcgagcca ggttcgtgga ggtggaccgg ctaccgggtt cgcatcgagg cgacggcggg
     4441 tacggctcca ccggcggcat ggccgcacac cggccggtcc aggaggacgt gtcgtgatct
     4501 tccgtcggcg caagtcgcga ccggacgccg acgacgcctc cgaactcgtc gagtcgccgg
     4561 acgaggggca acccgaagag ctggacgagc tggacgaact gtcgcgggcg cggccgcatg
     4621 gtccgtggga ccggtcggag acgaaggcgg acgaggacga cccggcgtac gtcgacctcg
     4681 gcggactggt ggtacgcggc cgcgagggtc tcgagctgcg tctccaggtg aacgaggcca
     4741 cacagctggt ggaggcggtc atgttggcgg gcgccgagtc ggggctggag ctgcgcgcgt
     4801 tcgcggcgcc tcggtcggcc agcatctggg acgacgtacg tcgcgacatc gccgccgagg
     4861 catcgaagcg gggcgggacg gctaccgagg tggacggcga gttcggtacc gagctgaagg
     4921 tcgtgcttcc tgtccagacg gcgcagggca ggcaggcgac gcagcccacc cgcatcgtcg
     4981 gcgtcgaagg cccccgctgg ctgctgcggg gcacgttcct cggggcgagt gcacagcagg
     5041 ccgatcccga cggcgtggtc gaaaacgcct tccgggacgt gatcgtggtc cggggggacg
     5101 gaccgatggc gccgcgcgac atgatcgcga tgcggatgcc tcgtggcgcc gaagtcaccg
     5161 cgacctccga gccggagccg accgacgaac tctgacgggc cgcagatcga gatggttcat
     5221 gtagccgggg gtctcgacgg gctcgaccgc cgggaaccag gggtctcgag ggactcgctg
     5281 atcgagcttg tcgagatcaa gagtccggga gaaccgctga tcgaggcagt tcatgtagcc
     5341 gggggtctcg acaggctcga ccgccgtagc ccggggtctc gacaggct
//
LOCUS       DATBFZ010000036         3132 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_1663531, whole genome shotgun sequence.
ACCESSION   DATBFZ010000036 DATBFZ010000000
VERSION     DATBFZ010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 3132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3132
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1663531"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            194..1138
                     /locus_tag="VH419_01085"
     CDS             194..1138
                     /locus_tag="VH419_01085"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HEY7042239.1"
                     /translation="MGDGGRVQYKTVVYDSSRWDRVVFRPGDIVITTPAKCGTTWLQM
                     ICALLVFQTPDLYAPLDTISPWVDMLTKDLPSVLATLEAQEHRRFMKTHTCFDGLPFD
                     ERVTYICVGRDPRDVAVSWDNHRANTDMIAFLAARERAVGLDDIADLLADGPPVEPEA
                     WGDRFWAWVENEEPVGAASSLHTTLDHLGQAWGRRERPNVLLLHYQDLTDDLDGQMRV
                     IANRLGITVPETTWPELVEAATFDRMRERATEIAPETPKRLWLNERDFFRQGRSGQWR
                     DLLDDTDVARYAAAVERWADPEVAAWVHRPPLPAGPST"
     gene            complement(1135..2160)
                     /locus_tag="VH419_01090"
     CDS             complement(1135..2160)
                     /locus_tag="VH419_01090"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEY7042240.1"
                     /translation="MKAVRLEDGAIRIREVDPPVPGQDQALVRITAAGVCHSDLHLAR
                     SDWEGIQTHTIGHEAIGVVEALGPGADRFVNVGDRVILGLGGMGGGYWCGACEFCLSG
                     RPRFCRLTKGILGTFAELFAVYAPSLVTLPDSISDDEAPLACGGLTAYGAVKKLQKHQ
                     VQPGRPIAVIGAAGGLGHYAVQLATAFGYEVVGVDVGAERLDFVKSLGARLAVDAGDA
                     LDVIRQEFGGVDASLVFAARLSGFDLGFKALRPSGVFIAVGLPPTSDGSFSITPWELI
                     GKDAVIASSAVGTVQDMRELIALASAGRVRSHISRRAKLSELESVFDDLQAGRYVGRA
                     VLTDLAN"
     gene            complement(2214..>3132)
                     /locus_tag="VH419_01095"
     CDS             complement(2214..>3132)
                     /locus_tag="VH419_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017936266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEY7042241.1"
                     /translation="FVADLEPTEDGSLQIMYGIDGRRDLTESTLDNLTGYVGAKPVRI
                     GNGAFDQRQNDVFGAVLDSVLLHTGRSQRLPRRVWPIVEAQARCATSIWQQPDQGIWE
                     ARGAPRHYVSSKLMCWVALHRAAKLSALRGINELEAEWGATADEIRADVLKQGVSDRG
                     VLRQHYETDSLDASTLLAPMFGFLEDEDEIAHNTVLAIADELTENGYVLRYRTDETDD
                     GMSGKEGTFLICSFWLVSALAIVGEGSRARDLMERLLRVASPLGLYAEEYDVDTGYHL
                     GNFPQAFSHLALIEAAGRIIANERLRELA"
BASE COUNT          504 a         1104 c         1055 g          469 t
ORIGIN      
        1 tgccacttgt cctggtgctc cacctcggcc gccgccaccc ggtagcgccg ctgcagacct
       61 tcgacgatcg gccgcaccgc cgtgcgcttg tccttcagcg agtggcagtc gggcaggtgg
      121 aggtcgaagg cggcaacggc ggcgaacaca gccggctcag cgtaaggccc gtgcggtcgg
      181 caaaactggc gcgatgggcg acggtgggag ggtccagtac aagacggtcg tctacgacag
      241 cagtcggtgg gaccgcgttg tcttccggcc cggtgacatc gtcatcacga cgccggccaa
      301 gtgcgggacg acgtggctgc agatgatctg cgcactcctc gtgttccaga cgcccgacct
      361 gtacgcgccg ctggacacca tctcgccgtg ggtcgacatg ctcaccaagg acctgccgtc
      421 ggtgctcgcc accctggagg cgcaagagca tcgccgcttc atgaagaccc atacgtgctt
      481 cgacggcctg cccttcgacg agcgcgtgac ctacatctgc gtcgggcgtg atccacgcga
      541 cgtggccgtg tcatgggaca accaccgggc caacaccgac atgatcgcct tcctggcggc
      601 gcgggagcgg gctgtgggcc tcgacgacat cgccgacctg ctcgccgacg gcccgcccgt
      661 ggagcccgaa gcctggggtg accggttctg ggcttgggtc gagaacgagg agccggtcgg
      721 cgccgcttcc tcgctgcaca ccacgctcga ccacctcggt caggcctggg ggcgtcggga
      781 gcgaccgaac gtgctgctgt tgcactacca agacctcacc gacgacctcg acggccagat
      841 gcgagtcatc gccaaccggc tcggcatcac cgtgcccgag acgacgtggc ccgagctggt
      901 cgaggccgcc accttcgacc gcatgcgcga gcgcgccacc gagatcgccc ccgagacgcc
      961 gaagcgactc tggctgaacg agcgagactt cttccgccag ggccggtccg gccagtggcg
     1021 cgacctgctc gacgacaccg acgtcgcccg gtacgcggcc gccgtcgagc gctgggccga
     1081 ccccgaagtc gcggcctggg tccaccgccc gccgttgccg gccggcccgt cgacctagtt
     1141 cgcgaggtcg gtgaggacgg cgcgtccgac gtagcggccg gcttggaggt cgtcgaacac
     1201 cgactccagc tccgacagct tcgcccgacg cgagatgtgg ctgcgcacgc gccccgcgga
     1261 agccagcgcg atcagctcgc gcatgtcctg cacggtgccg accgctgacg acgcgatcac
     1321 cgcgtccttg ccgatgagct cccagggggt gatcgagaag cttccatcgc tggtcggcgg
     1381 caggccgacc gcgatgaaga cgccactcgg tcgcaacgcc ttgaagccga ggtcgaagcc
     1441 cgacagtcgc gccgcgaaga ccagcgacgc atcgacgccg ccgaactcct ggcggatgac
     1501 gtcgagggcg tcaccggcat cgaccgcgag gcgggccccg agcgacttca cgaaatcgag
     1561 ccgttcggcg ccgacatcga cgccgacgac ctcgtacccg aaggcggttg ccagctgcac
     1621 ggcgtagtgc ccgaggccgc cggcggcgcc gatcaccgcg atcggccggc ccggttggac
     1681 ctggtgcttc tgcagcttct tgaccgcgcc atacgcggtc agcccgccgc acgccaacgg
     1741 ggcctcgtcg tcgctgatgc tgtcgggaag cgtgacgagt gacggcgcgt acacggcgaa
     1801 cagctccgcg aacgtgccga ggatcccctt ggtcagacgg cagaagcgag ggcgtccgct
     1861 caagcagaac tcacaggcac cacaccagta cccgccaccc atgccgccga gcccgaggat
     1921 gacgcggtcg ccgacgttca cgaaccggtc ggctccgggc ccgagcgctt cgaccacgcc
     1981 gatggcctca tggccgatcg tgtgggtctg gatgccctcc cagtcggagc gggcaaggtg
     2041 gagatcggag tgacacacac ccgcggccgt gatccgtacg agtgcctggt cctgacccgg
     2101 caccggcgga tcgacctcac ggatgcggat cgcaccatcc tcgagacgaa ctgccttcat
     2161 gcctctgccc ccgcgcgttc gagtcgcttc ggcagcgtag ccagccgatc cgactaggcg
     2221 agctcgcgca ggcgctcgtt ggcgatgatc cgtcccgccg cctcgatcag cgcgagatgc
     2281 gagaaggcct gtgggaagtt cccgaggtgg tagcccgtgt cgacgtcgta ctcctcggcg
     2341 tacagcccga ggggagacgc gacgcgcagc aagcgctcca tcagatccct tgcccgtgat
     2401 ccctcgccca cgatggcgag cgccgacacg agccagaacg agcagatgag gaaggtgccc
     2461 tccttgccgg acatgccgtc gtcggtctcg tcggtccggt agcgcagcac gtagccgttc
     2521 tcggtgagct cgtcggcgat ggcgaggacg gtgttgtggg cgatctcgtc ctcgtcctcg
     2581 aggaacccga acatcggggc cagcagggtg gacgcatcga gcgagtcggt ctcgtagtgc
     2641 tgccgcagca cgccgcgatc gctgactccc tgcttcaaca cgtcggcgcg gatctcatcg
     2701 gcggtcgcac cccattcggc ctccagctcg ttgatgccgc gcagggccga gagcttcgcg
     2761 gctcggtgga gggccaccca gcacatcagc ttcgacgaga cgtagtgacg aggtgcgccg
     2821 cgcgcctccc agatgccctg gtccggttgc tgccagatgc tcgtcgcgca acgggcctgg
     2881 gcctccacga tcggccagac ccggcgcggc aggcgctggc tgcgtccggt gtggagcagc
     2941 accgagtcga gcacggctcc gaagacgtcg ttctggcgct ggtcgaacgc accgttgccg
     3001 atccggaccg gcttcgcgcc gacgtagccc gtgaggttgt cgagcgtcga ttccgtcagg
     3061 tcacggcggc cgtcgatccc gtacatgatc tggagcgagc cgtcctcagt cggttcgagg
     3121 tctgcgacga ac
//
LOCUS       DATBFZ010000037         1988 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_2321282, whole genome shotgun sequence.
ACCESSION   DATBFZ010000037 DATBFZ010000000
VERSION     DATBFZ010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 1988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1988
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2321282"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..555)
                     /locus_tag="VH419_01100"
     CDS             complement(28..555)
                     /locus_tag="VH419_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008363669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HEY7042242.1"
                     /translation="MPRGTPIRPDAGDHVDNDALLRRHGLQVTAQRLAVLRAVTRHSH
                     STADDIDKAVRAEIGAISRQAVYDALAALTETRILRRIQPAGSAARYETRVGDNHHHL
                     ICRTCGQMVDVDCAVGYTPCLTASDDSGYEIDEAEVVYWGRCPDCQAASDGDGRGSGA
                     KSSKRKTPREENRRV"
     gene            753..980
                     /locus_tag="VH419_01105"
     CDS             753..980
                     /locus_tag="VH419_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015071955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HEY7042243.1"
                     /translation="MKLSLSMSEDDVEFIDQYASEHGVHSRSAVVQRAVSLLRAGELG
                     DDYAAAWTEWDRTDAELWDEAVGDGVESGTP"
     gene            977..1324
                     /locus_tag="VH419_01110"
     CDS             977..1324
                     /locus_tag="VH419_01110"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015071954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEY7042244.1"
                     /translation="MRRGDVVWVDLEPVRGSEANKRRPAVVVSNDGANRAAQSLGRGV
                     ITVVPITSTTGRVYPFQVRIPAKTSGLPKASKARAEQIRSIAVQRVVSTAGHLAPELL
                     EQLDDAIRLHLSL"
     gene            complement(1410..1577)
                     /locus_tag="VH419_01115"
     CDS             complement(1410..1577)
                     /locus_tag="VH419_01115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family DNA-binding protein"
                     /protein_id="HEY7042245.1"
                     /translation="MGAEPLRATEAARQLGIPTQELLRLIHEKQIRYVMVDGIAHVPA
                     DAIDEFRTRAS"
     gene            complement(1567..1773)
                     /locus_tag="VH419_01120"
     CDS             complement(1567..1773)
                     /locus_tag="VH419_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042246.1"
                     /translation="MSADLPEPPPAIRYSLDEALSLLAALEDGRDALIDSGHLAVVVS
                     VEAEIRLLSRRLGFDDPEGDANGR"
BASE COUNT          297 a          698 c          667 g          326 t
ORIGIN      
        1 actgggtttt cgctttcgct gccggtgtca gacacgccgg ttctcctctc ggggtgtttt
       61 cctcttcgag ctcttcgctc ccgacccgcg gccgtcgccg tcggacgccg cttggcagtc
      121 ggggcagcgg ccccagtaga cgacctcggc ctcgtcgatc tcgtagccgg agtcgtcgga
      181 cgccgtgagg cacggcgtgt agccgaccgc gcagtcgacg tcgaccatct gcccacacgt
      241 gcggcagatg agatggtgat ggttgtcgcc cacccgggtc tcgtagcggg ccgccgaccc
      301 cgcgggctgg atgcgccgga ggatgcgggt ctcggtgagc gcggccaggg cgtcgtagac
      361 ggcctgccgg gagatggccc cgatctcggc ccgcaccgct ttgtcgatgt cgtccgccgt
      421 gctgtgcgag tggcgggtga ccgcgcgcag cacggccagg cgctgggccg tgacctgcag
      481 accatgccgg cggagcaggg cgtcgttgtc gacgtgatcg ccggcatcag gccgtatcgg
      541 cgtccccctg ggcacagccg gaccctaacc ccgattctgg agtcagtcca gatctagccc
      601 gatcccgcca cgcgcacccg gtccggcctt cgagcgcgca cagatggtcg ctctgcggcc
      661 atgactgcac tctcgtcaga tcaacgaggc cggcgaccct ccgggtagta caacggtagc
      721 tcctccgcta ctaccacggt acgatctccg gcgtgaagct cagcctgagc atgtccgagg
      781 acgacgtcga gttcatcgac cagtacgcca gcgagcatgg cgtccactca cggtcagctg
      841 tcgtacaacg ggcggtgtcg ctgctgcggg ccggcgagct cggcgacgac tacgcggcgg
      901 cctggacgga gtgggaccgc accgacgccg agctgtggga cgaagccgtc ggtgacggcg
      961 tcgagtccgg cacgccatga gacgcgggga cgtcgtctgg gtggacctcg agcccgtgcg
     1021 cggcagcgag gcgaacaagc gccgcccggc ggtcgtcgtc agcaacgacg gcgccaaccg
     1081 cgccgcgcag agcctcggcc gcggcgtcat cacggtggtc ccgatcacct cgacgaccgg
     1141 tcgggtctac ccgttccagg tccgcatccc ggccaagact tccggcctgc cgaaggcgtc
     1201 caaggcacgg gctgagcaga tccgatccat cgccgtccag cgcgtcgtca gcaccgccgg
     1261 acaccttgcc ccggagctgc tcgagcagct cgacgacgcc atccggcttc acctcagcct
     1321 ctagccaaac cgcgaacgat gccggtgcgg gagcccgaca ccacgaccgg cgctaccgaa
     1381 cgctcctgcg tcagtacggc agatccgttt caggacgccc tggtccggaa ttcgtcgatc
     1441 gcgtcggcag gcacgtgcgc gatgccgtcg accatcacgt atcggatctg cttctcgtgg
     1501 atgagtcgga ggagttcctg cgtcggtatc ccgagttggc gggcggcctc ggttgctcgc
     1561 agtggctcag cgcccattgg cgtctccctc gggatcgtcg aagccaagcc tacgactgag
     1621 cagcctgatc tcggcctcca cgctcacgac gactgcgaga tggccgctgt cgatcaacgc
     1681 gtcccggccg tcctcgagcg cggccagcag gctaagagcc tcatcgaggc tgtaccggat
     1741 cgccggcggc ggctctggaa ggtcggcgct cacggctcca tcatgacggc ccggtgtgac
     1801 acaacttggg tgacgcgtct tgcccgctcg ccacgatcgc ccgtcgagcg ccgagcggca
     1861 gctcggcgcg tggcgggtct cgatctccag atcgagtgtg catccgtgtg ccggacagac
     1921 acgtgtgtgc acactcgacg gcacgcgacc gatcgcccgt tctggtgtgc ccgtgcacgc
     1981 cgttgaac
//
LOCUS       DATBFZ010000038         1532 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_1008650, whole genome shotgun sequence.
ACCESSION   DATBFZ010000038 DATBFZ010000000
VERSION     DATBFZ010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1008650"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(110..>1532)
                     /locus_tag="VH419_01125"
     CDS             complement(110..>1532)
                     /locus_tag="VH419_01125"
                     /inference="COORDINATES: protein motif:HMM:NF012956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4982 domain-containing protein"
                     /protein_id="HEY7042247.1"
                     /translation="FAGQFLWSGWDYIGEPTPYTVFPVKTSFFGAVDTAGFPKDAYYL
                     YKSQWNPAPMVHILPMNWTDYKRGQPVQVWAYANEPTVELFLNGVSLGAKSFTRKTAS
                     DGLRYLETTECPGDDKTYTTGACPGSYESPNGSSGKLHLAWTVPFAPGKLVAVARDAA
                     GKVVARDEVDTAGAPDALKLTPDKTVLRADGKSLSYVTVHVVDARGIEVPDADNPIHI
                     SVRGAGSFAGADNGKQDDAEGYKSPTHDAFNGKLLAIVQAGTRPGPITIAASADGLHG
                     AAMTLRASDRPSGDGPRAHSVRLRRSHRALSPRVASTAPVADASFSGGVFSDGGSDFG
                     TSSTLPATMLDGNPATFWSNRYSKGATQTLPEITNSHPSDWVSVSWRAPQRVDQIQAL
                     FVVDANDQLPATVTVSYWDGSSWKPVSDQNIAFAGSSGAPSSITFDPVTTTKLKLDMS
                     SASPDDPTTGNLAIAELRIPGVT"
BASE COUNT          233 a          501 c          529 g          269 t
ORIGIN      
        1 gcctcatttg cagccaccca gcgacagatc gctcagctgc cggtgacgta cgagcgcgaa
       61 gaccaagccg cgtagcgaca gtccttacga cacctcatcc agagcccgcc tacgtcacgc
      121 ccgggattcg cagctcggca attgcgaggt tgccggtcgt gggatcgtcg ggcgaggcgc
      181 tggacatgtc gagcttgagc ttggtcgtgg tgaccgggtc gaacgtaatc gacgacggtg
      241 cgccggagct tcccgcgaac gcgatgttct gatcgctgac gggtttccac gagctgccgt
      301 cccagtaaga gacggtgacc gtcgccggca actggtcgtt ggcatcgacg acgaacagcg
      361 cctggatctg gtcgacccgt tgcggagccc tccagctgac gctcacccaa tcgctcgggt
      421 gcgagttggt gatctccgga agcgtttggg tcgcgccctt gctgtagcgg tttgaccaga
      481 acgtcgcggg gttgccgtcg agcattgttg ccggaagcgt cgagctcgtc ccgaagtccg
      541 agccgccgtc ggagaacacg ccgccggaga agctcgcgtc cgcgactgga gcggttgacg
      601 cgacgcgagg ggagagcgcg cggtggctgc gccgcaggcg gaccgagtgg gcgcgcggac
      661 cgtcaccgct gggtcggtcg ctggcgcgca gcgtcatcgc cgcaccgtgc agcccgtcgg
      721 cggatgccgc gatcgtgatc ggtcccggcc tggtgccggc ctggacgatc gccagcagct
      781 tgccgttgaa cgcgtcgtgc gtgggggact tgtagccctc ggcgtcatcc tgcttgccgt
      841 tgtcggcgcc ggcgaagctg cccgcgccgc ggaccgagat gtggatcggg ttgtcggcgt
      901 cgggcacctc gatgccgcgc gcgtcgacca cgtggacggt gacataggac agcgactttc
      961 cgtcggctcg gagcacggtc ttgtccgggg tcagcttcag ggcgtccggc gcgcctgccg
     1021 tatcgacctc gtcgcgcgcg accaccttgc cggccgcatc cctggcgacg gcaacgagct
     1081 tccccggcgc gaacggcacc gtccacgcca ggtgcagctt gcccgagctg ccattcgggc
     1141 tctcatagct cccagggcag gcaccggtcg tgtaggtctt gtcgtcgccc gggcactcgg
     1201 tcgtctccag atagcggagt ccgtcgctgg ccgtcttgcg cgtaaacgac tttgcgccga
     1261 gcgagacgcc gtttaggaac agctccaccg tcggctcgtt ggcgtacgcc cagacctgca
     1321 ccggctggcc ccgcttgtag tcggtccagt tcatcggaag gatgtgcacc atcggcgccg
     1381 gattccactg agacttgtag aggtagtagg cgtccttcgg gaatccggcg gtgtcgacgg
     1441 cgccgaagaa cgacgtcttc accgggaaga ccgtgtatgg agtcggctcg ccgatgtagt
     1501 cccaccccga ccacaggaac tgcccggcaa ag
//
LOCUS       DATBFZ010000039         1605 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_1008713, whole genome shotgun sequence.
ACCESSION   DATBFZ010000039 DATBFZ010000000
VERSION     DATBFZ010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1008713"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(183..>1605)
                     /locus_tag="VH419_01130"
     CDS             complement(183..>1605)
                     /locus_tag="VH419_01130"
                     /inference="COORDINATES: protein motif:HMM:NF012956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4982 domain-containing protein"
                     /protein_id="HEY7042248.1"
                     /translation="FAGQFLWSGWDYIGEPTPYTVFPVKTSFFGAVDTAGFPKDAYYL
                     YKSQWNPVPMVHILPMNWTDYKQGQPVQVWTYANEPTVELFLNGVSLGAKSFTRKTSG
                     DGLRYLETTECPGDDKTYTTGACPGSYESPNGSSGKLHLAWTVPFAPGKVVAVARDAT
                     GKVVARDEVDTAGAPDALKLTPDKTVLRADGKALSYVTVHVVDEHGIEVPDADNTIHV
                     SVRGPGSFAGADNGKQDDAEGYKSPTHDAFNGKLLAIVQAGTRPGPITITASADGLHG
                     AAITLRASDRPRGDGPRADSVRLSRSHRAFGPRVASTAPAADASFSGGVFSDGGSDFG
                     TSSTLPTAMLDGNPATFWSNRYSKGATQTLPEITNSHPSDWVSVSWGAPQRVDQIQAL
                     FVVDANDQLPTTITVSYWDGSSWKPVSDQSIAFAGSSGAPSSITFDPVATTKLKLDMT
                     SASPGDPMTGNLAIAELRIPGVT"
BASE COUNT          242 a          536 c          546 g          281 t
ORIGIN      
        1 ccacgagcac agctggagcc ctccgggcca ttttgctgtg gtctactgca cacgctgcgg
       61 cctgacgctc agcgcctcat ttgcagccac ccagcgacag atcgctcagc tgccggtgac
      121 gtacgagcgc gaagaccaag cggcgtagca acagtcctcc cgacacctca tctagagccc
      181 gcctacgtca cgcccgggat tcgcagctcg gcaattgcga ggttgccggt catgggatcg
      241 ccgggcgacg cgctggtcat gtcgagcttg agcttggtcg tggcgaccgg gtcgaacgta
      301 atcgacgacg gcgcgccgga gctgcccgcg aacgcgatgc tctgatcgct gacgggtttc
      361 cacgagctgc cgtcccagta agagacggtg atcgtcgtcg gcaactggtc gttggcgtcg
      421 acgacgaaca gcgcctggat ctggtcgacc cgttgcggag ccccccagct gacgctcacc
      481 caatcgctcg ggtgcgagtt ggtgatctcc ggaagagtct gggtcgcgcc cttgctgtag
      541 cggtttgacc agaacgtcgc ggggttgccg tcgagcatcg ccgtcggaag cgtcgagctc
      601 gtcccgaagt ccgagccgcc gtcggagaac acgccaccgg agaagctcgc gtccgcggct
      661 ggggcggttg acgcgacgcg agggccgaac gcgcggtggc tgcgcgacag gcggaccgag
      721 tcggcgcgcg ggccgtcgcc cctgggtcgg tcgctggcgc gcagcgtaat cgccgcaccg
      781 tgcagcccgt cggcggatgc cgtgatcgtg atcggtcccg gcctcgtgcc ggcctggacg
      841 atcgccagga gcttgccgtt gaacgcgtcg tgcgtggggg acttgtagcc ctcggcgtca
      901 tcctgcttgc cgttgtcggc gccggcgaag ctgccgggcc cgcggaccga gacgtggatc
      961 gtgttgtcgg cgtcgggcac ctcgatgccg tgctcgtcga ccacgtggac ggtcacgtag
     1021 gacagcgcct ttccgtcggc tcgcagcacg gtcttatccg gggtcagctt cagggcgtcc
     1081 ggcgcgcctg ccgtatcgac ctcgtcgcgc gcgaccacct tgccggtcgc atccctggcg
     1141 acggcaacga ccttccccgg cgcgaacggc accgtccacg ccaggtgcag cttgcccgag
     1201 ctgccgttcg ggctctcata gctaccaggg caggcgccgg tcgtgtaggt cttgtcgtcg
     1261 cccgggcact cggtcgtctc cagatagcgg agtccgtcgc cggacgtctt gcgcgtaaac
     1321 gactttgcgc cgagcgagac gccgttcagg aacagctcca ccgtcggctc gttggcgtac
     1381 gtccagacct gcaccggctg gccctgcttg tagtcggtcc agttcatcgg aaggatgtgc
     1441 accatcggca ccggattcca ctgcgacttg tagaggtagt aggcgtcctt cgggaatccg
     1501 gcggtgtcga cggcgccgaa gaacgacgtc ttcaccggga agaccgtgta tggagtcggc
     1561 tcgccgatgt agtcccaccc cgaccacagg aactgcccgg caaag
//
LOCUS       DATBFZ010000040        10702 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_2323409, whole genome shotgun sequence.
ACCESSION   DATBFZ010000040 DATBFZ010000000
VERSION     DATBFZ010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 10702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10702
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2323409"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            192..554
                     /locus_tag="VH419_01135"
     CDS             192..554
                     /locus_tag="VH419_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042249.1"
                     /translation="MGDSGYPGAVRLDVQLGAGKARMTMTNLSLLDYFSIPNALFHFQ
                     DPVAAVCSFDIRWSGPVTDRSPVVAPAGSEGDLVLSSATMTWSASNDLGFSFESDPTP
                     TSSIFAQLGRVRNGVFAD"
     gene            908..1336
                     /locus_tag="VH419_01140"
     CDS             908..1336
                     /locus_tag="VH419_01140"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEY7042250.1"
                     /translation="MGVIDGRTWLEHLSKAECWKLLALHPVGRIGVLVDSAPEIYPVN
                     HVVDRGTIVFRTDPGTKLRGLDRSPSVCYEVDGLSLEERAGWSVLVKGRAVELTSSDE
                     LRAAQELPLCFWSVGEKAHWIRIVPREVTGRRIHQPAPQR"
     gene            1478..1675
                     /locus_tag="VH419_01145"
     CDS             1478..1675
                     /locus_tag="VH419_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008355884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1918 domain-containing protein"
                     /protein_id="HEY7042251.1"
                     /translation="MRASVGDRIVVKSRHIDEPNRDCEVLEVHGADGGPPYLVRWGDT
                     GHRTLFFPGSDAVVQHFEHSS"
     gene            1672..2127
                     /locus_tag="VH419_01150"
     CDS             1672..2127
                     /locus_tag="VH419_01150"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY7042252.1"
                     /translation="MTCSGRIVVGVDASEGSAVALRWALEEARLRGSTVEVVHAWHYP
                     YLASCDITGMAPAGLSLGDLEAGERAVLDRAVAAGGTAPEGVTVNPILADGGAAEVLL
                     EAAVGADLLVVGSRGHGGFAGLLLGSVSEHCVHHSPCPVVVVPHGRPTR"
     gene            complement(2356..3318)
                     /gene="argF"
                     /locus_tag="VH419_01155"
     CDS             complement(2356..3318)
                     /gene="argF"
                     /locus_tag="VH419_01155"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEY7042253.1"
                     /translation="MKDVLRIADLSPDDLRHGIGLALAAKRRPREWGSLLDGETAVLC
                     FAMPATRSRILFESAVARLGGTPVVLGPTELQLGRDDSLEDAAKDISRFARAIVIRGF
                     DDKDLRRFAAAATAPVINASSAGHHPCQALTDLMTLREHFGRLSGLKVAYLGAGNNVA
                     HSLIEACALAGIDIAVATPPGFGPSVEVLDHADQVARVSGSIVWPTHDPLVAVAGANA
                     VYTGPWSSMNTSEQERELRVAALRPYEVTSAVMAEADPGAVFLHCLRTRRGEEVSPAV
                     IDGPQALVVEQAENRLHAAVGLLAGLLKEELAGATAGSREPQPA"
     gene            3563..4036
                     /locus_tag="VH419_01160"
     CDS             3563..4036
                     /locus_tag="VH419_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042254.1"
                     /translation="MTPPFDVAAFLSAHREPILDRATTEVAARRLPHYEATGLGETQA
                     RLAALFDVVTRAAGEKDLAPVFAYTEELARSRQRTGHGLSEVQTAINALEEQLWRAVL
                     DDAPPDVQGLGLAMVSTILGAVKDRLACDYVARVAGQPMRTLLVEELFRGTAGPP"
     gene            complement(4083..4691)
                     /locus_tag="VH419_01165"
     CDS             complement(4083..4691)
                     /locus_tag="VH419_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042255.1"
                     /translation="MRTSRWWMVAAAVAVSVVVTSVALLRQDDGKVDATNQSPTTTVV
                     TSSTTTGPSTTDTTAETTTTTIPPSSPTTAAPPTLLAPALEVAPGAGSGEVQVDWKAV
                     AGATGYRVFRADDRQGPFAVIVDVNISNGRVTAVPSVVNVYSEAHSYVPQQGRMSGPD
                     RSLSFHVVQVESGQRGGPCYRVAAYDSTGQGASSSIHCASPR"
     gene            4757..5056
                     /locus_tag="VH419_01170"
     CDS             4757..5056
                     /locus_tag="VH419_01170"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY7042256.1"
                     /translation="MPAEDADPPGDLEAGERAVPDRAVAAGGTAPEGVTVNPILADGG
                     AAEVLLEAAVGADLLVVGSRGHGGFAGLLLGSVSEHCVHHSPCPVVVVPHGRPPR"
     gene            complement(5071..5397)
                     /locus_tag="VH419_01175"
     CDS             complement(5071..5397)
                     /locus_tag="VH419_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042257.1"
                     /translation="MPDDGLRTIAIHRALGRVVVAPSGELSRDSTLLLRTILGDLVAG
                     QGNLELVIDAMDVVHMHAEVVTVLSEIAVEAAARGAVVVVSLPPDDARAELERAGVKT
                     VPRPGA"
     gene            complement(5470..6513)
                     /locus_tag="VH419_01180"
     CDS             complement(5470..6513)
                     /locus_tag="VH419_01180"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEY7042258.1"
                     /translation="MRALVYHGPGQKAWTEVPDPAIGADTDAIVRVDAVTICGTDLHI
                     LKGDVPTVTSGRVLGHEAVGTVEAVGAGVKTVAVGDRVLVSCITSCGACRFCREGRYG
                     QCLGGGGWILGHLVDGTQAEYVRVPFADTSTHKVPPGVSDDQILMLADILPTGYEVGV
                     LNGRVQPGDVVAVVGAGPIGLAAMMGARLFSPSHVVAIDLADSRLEAAKQFGADNVVN
                     NGREDARAAVLGLTNGLGADVSIEAVGVPAAFELAVALARPGGHIANIGVHGEPSTLH
                     LEEQWIRDITITTGLVDTYTTPTLLRLVATGQLDSTRFVTHHFGLDEFEDAYDVFSRA
                     AETGALKVVLTRP"
     gene            6670..7335
                     /locus_tag="VH419_01185"
     CDS             6670..7335
                     /locus_tag="VH419_01185"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEY7042259.1"
                     /translation="MKVRDVMTTEVVTVRPETPFAEIVSQLLWQGISGVPVVDDDGRL
                     LGVVTEADLVSNEAYGYRRRRALALIGDYLRGFDPGWVRKASARTARELMTAAPDVAR
                     PDEQVADVARRMLEERHKRLPVVEDGRVVGIVSRHDLLRQFHRPDEETAGDIERLLAD
                     PFRAPETHAAAARVENGIVTLEGTVQRPSDKRVVEAAVAQVPGVVAVDNRLEAREPEP
                     RAF"
     gene            7558..7824
                     /locus_tag="VH419_01190"
     CDS             7558..7824
                     /locus_tag="VH419_01190"
                     /inference="COORDINATES: protein motif:HMM:NF020532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dsRBD fold-containing protein"
                     /protein_id="HEY7042260.1"
                     /translation="MKEPRVWNVEVVFTEDADRTRADTVLDVGDLRIHGWGRARRRPG
                     DPDVPAIGEELAAARALADLAHQLLQHAADSIGAFEGHPVNVHG"
     gene            7844..8983
                     /locus_tag="VH419_01195"
     CDS             7844..8983
                     /locus_tag="VH419_01195"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEY7042261.1"
                     /translation="MATAAHEPANGSSPGSPWRAADARTQHRPGMSMRVATVSGIQIR
                     IHATFLVLVALVAVTANAEEGPGLTWGLIWLVALFSCVVVHELAHSLVARRFGIPVLE
                     IELLPFGGISKMGRSPQDPGVELRIALAGPLASVGLGAAFAWLALLAGASMWPPTLYG
                     GGFLARLAWVNLLLAGFNLVPALPLDGGRVLRATLEQRSDRDRATRVAARAGRLFAVA
                     MIAAGLLFNLWLLVIGLFVYAGSWAEEAAALLHACVKELHVSDVMVRDPMCVSSATPV
                     EELVADLWRTAQREFPVVGADGACEGVVTADALLRAAPHARVGALADARAVLAPDDPL
                     ETCGLLTGEIGAAAVVSGGQVVGLARAADTAAVVRQLLRQGAQKP"
     gene            9426..9620
                     /locus_tag="VH419_01200"
     CDS             9426..9620
                     /locus_tag="VH419_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042262.1"
                     /translation="MRLVTADDWDPALASLRLREVVSDALVLRQMSARVQRAIAERPS
                     EVAERAARTLAAAMGVAQST"
     gene            9765..10247
                     /locus_tag="VH419_01205"
     CDS             9765..10247
                     /locus_tag="VH419_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042263.1"
                     /translation="MTWLVLLAESAIAVSLIVYLVHDRRLDDDAAFDAYVDVEDVPPG
                     ADDRAGPPLHLAVDEADDGRLTAEIVFCEVQPGTLRLDCVVSAGEGRGWPFSIALELP
                     NNDSALSVAWAIGQWIATSAVVVLRLPPEEADHPYVDLTDGITTVRLETTNHGPLLCE
                     "
     gene            10333..10698
                     /locus_tag="VH419_01210"
     CDS             10333..10698
                     /locus_tag="VH419_01210"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEY7042264.1"
                     /translation="MEHHHEVLQELRDPTKALRRAIPETWAGFVALHDAAMAEGEVPA
                     RLKEAAALAISAVKRCDGCIAYHAKAAAHAGATPGEVAELLGVALLMDGGTASVYAPR
                     AWEAYAEFVREYTGMEAVS"
BASE COUNT         1609 a         3661 c         3801 g         1631 t
ORIGIN      
        1 ctcggcgtcg aggtcctccg gtgcaacgcg gtcgagattt cgtcgtgctt ccgccaggag
       61 gcggtcgacc gctgactcgg acccggcggg atcgtcgggc ccagcggagt ccagcggctc
      121 gtcccgtgcg agcacgcgct ggagcttctg tgcgggcccg gtccgcggcg cccagcgcgc
      181 cgaagccgtg cgtgggggac tcggggtacc cgggggctgt acgcctcgac gtgcagctcg
      241 gcgcgggcaa ggctcggatg acgatgacca atctctcgct gctcgactac ttctcgatcc
      301 cgaacgcgct gttccacttc caagatcccg tggcggccgt gtgctcgttc gacatcaggt
      361 ggagcggccc ggtcaccgac cgctcgcccg tcgtagcgcc ggcgggatcc gagggggatc
      421 tggtcctgag cagcgcaacg atgacgtggt cggcgtcgaa cgacctcggg ttctcgttcg
      481 aatctgatcc caccccgacg tcgagcatct tcgcccagct cggccgcgtc cgaaacggcg
      541 tgttcgcgga ctgacgcagc cgcgagggat ccgcgttctc cacaactgag cgctacgcct
      601 gaccggtgcg tcgtcaccgg gccggagccc ccagttcggc aacgcagtgc tgtccagaag
      661 aggcggatcg acatcacggc agcgccatgg tccctcgggg ggctgaaagc ggccattcgg
      721 ccctacagca gaatgcggcc aatctctaca ccgggaccat ggagcgtgag accgaggctt
      781 ccacgacgtc gcgaacgccc gatcgacacg cccatacggt tcctccgcgg caagcccgga
      841 cttgtcctgc taccgggtcg acctgcggga ggtcggcccg aacgacatcg gctgaaggga
      901 ggctgccatg ggagtcatcg atggccgcac atggctcgag cacttgagca aggctgagtg
      961 ctggaagctc ctggcgctgc atcctgtggg ccgcatcggt gtcctcgtcg acagcgcacc
     1021 tgagatctat cccgtcaacc acgtggtcga tcgcggcacg atcgtctttc ggacagaccc
     1081 gggcacgaag ctacgcgggc tcgatcgcag cccttcggtc tgctacgagg tcgacggcct
     1141 gagcctcgag gagcgcgccg ggtggagcgt gctcgtcaag ggccgagccg tcgagctgac
     1201 ctcctcggac gagttgcgcg cggcacagga gctgccgctt tgcttctggt cggtcggcga
     1261 gaaagcgcat tggatacgca tcgttccacg cgaagtcacc gggcgccgga tccatcagcc
     1321 cgcgccacaa cgctgaacgg ccgtcatggt gccactgcga gcgccgtggc atcgccacca
     1381 gagtccttcg cccaccaccc ttggatccat gacgttcgcc tctacctgtg aacgcccggg
     1441 tggtagcacg atcgggacag aaccgaaggg agtcgccatg cgagcaagtg tgggcgaccg
     1501 aatcgtcgtc aagagccgtc acatcgacga gccgaaccgg gactgcgaag ttctcgaggt
     1561 gcacggcgcc gatggcggac cgccctacct cgtgcggtgg ggtgacaccg gacatcggac
     1621 actcttcttc ccaggctcgg acgcggtcgt ccagcacttc gagcactcct catgacctgc
     1681 tccggccgca tcgtcgtcgg ggtggacgcg tccgaggggt cggccgtggc tctgcggtgg
     1741 gcgctcgaag aggcgaggct ccgcgggtcg accgtcgaag tcgtgcacgc gtggcattac
     1801 ccataccttg cgagctgtga catcacgggc atggctccgg ccggactctc gctcggcgat
     1861 ctggaggcgg gcgagcgcgc cgtgctcgat cgcgctgtgg cggccggggg aacggcgccc
     1921 gagggcgtga cggtcaaccc gatcctcgcc gacggcggcg cggcggaggt gttgctcgag
     1981 gcggccgtcg gagctgacct gctcgtcgtc ggctcacgcg gtcatggcgg gttcgcaggt
     2041 ctgctgctcg gctcggtgag cgagcactgc gtgcaccaca gcccgtgccc ggtcgtggtg
     2101 gtgccgcacg gccgaccgac gcgatagcgg cgcggtcaga gtcctgttgg ctcgcgggac
     2161 ggtcatcatg tcggcgcgct ccaactcgcg tcgcgcgtgt tccggcggaa ggctcaccac
     2221 gaccactgca ccccgcgact cgcagttcga cggcgtgagc cgccgaagct cgaggtcgga
     2281 ccggagcagc agcacgcgcc gagggttccg acctctgacc ctacgaagca gccggacatg
     2341 gggggtgcct tccgctcagg ccggctgcgg ttcgcggctc cccgccgtcg cgccggccag
     2401 ttcctccttg agcagaccgg cgagcagccc gaccgcggca tggagtcggt tctcggcctg
     2461 ctcgacgacc aacgcctgcg ggccgtcgat caccgccgga ctcacctcct cgccgcgacg
     2521 ggtacggagg cagtgcagga acaccgcgcc cggatcggct tcggccatga cggcgctcgt
     2581 gacctcgtac ggtcgaagcg ccgccacccg gagctcccgc tcctgctcgg acgtgttcat
     2641 cgacgaccac gggccggtgt acacggcgtt ggcgcccgcg acggccacga gcggatcgtg
     2701 ggtcggccag acgattgacc cgctcactct tgcgacctgg tcggcgtggt cgaggacctc
     2761 gaccgagggt ccgaaccccg gtggtgtcgc cacggcgatg tcgatgcccg cgagcgcgca
     2821 cgcctcgatg aggctgtgcg cgacgttgtt gccggcgccc agatacgcga ccttcaagcc
     2881 cgagaggcga ccgaagtgct ccctgagcgt catcagatcc gtgagcgcct ggcacgggtg
     2941 atgaccggcg gacgaagcgt tgatcaccgg agcggtcgcg gccgcagcga agcgtcggag
     3001 gtccttgtcg tcgaaccccc ggatgacgat ggctcgggcg aaacggctga tgtccttcgc
     3061 agcgtcttcg agcgagtcgt cccgaccgag ctggagctcc gtcggtccga gaacgacggg
     3121 tgtgccgccg agccgggcca cggcgctctc gaacaggatt cgactgcgcg tcgcgggcat
     3181 cgcgaagcac agcaccgcgg tctcgccgtc gagcaaggac ccccactccc gcggccgccg
     3241 cttggcggca agcgccagtc cgatgccatg tcggagatcg tccgggctga ggtcggcgat
     3301 gcgtagcacg tccttcacgg ttcgagcgtg cgcgccgcga tgttgtcgat gccagggccg
     3361 aagggctcta cccgctgtcc ggcgtgcagc tacctcagtg gcgtgcgcaa caccagaacg
     3421 tcccccacgt ccgcccggaa cagctgcagc tgcccgccga tccacacgac ggctgagtag
     3481 ccatcgctgc ggtcgggagc aggcccgaca gatcgagacg ctcgccgcct cgacgacggc
     3541 ttcctggact agaccacgcg ccgtgactcc cccattcgat gtggccgcct tcctgtccgc
     3601 acaccgagag ccaatactcg acagagcgac cactgaggtc gccgcgcgcc gactgccaca
     3661 ctacgaggcc accggtctcg gggagaccca ggcgcgcctg gccgccctct tcgacgtcgt
     3721 gacacgggcc gcaggcgaga aggacctcgc acccgtcttc gcctacaccg aggagctcgc
     3781 gcggagccga caacgtaccg gccacggcct gagcgaggtg cagaccgcga tcaacgcctt
     3841 ggaggagcaa ctctggcgtg ccgttctcga cgacgcacca cccgacgtcc agggactggg
     3901 tctcgccatg gtgtcgacga ttctcggcgc ggtcaaggac cggctggcat gcgactacgt
     3961 cgctcgcgta gccggtcaac cgatgcggac cttgctcgtc gaggaactct tccgcggcac
     4021 ggccggccca ccctgacccc gtgcgcccga ggccggactg cgagcttcga tgaccccggg
     4081 gttcagcgtg gtgatgcgca gtggatcgat gaggatgcgc cctgtcccgt gctgtcgtag
     4141 gccgcgaccc gatagcacgg accgccgcgc tggccagact ccacctgcac gacgtggaag
     4201 gacaacgatc ggtccggtcc gctcatccgg ccctgctgcg gtacgtagga gtgggcctcc
     4261 gagtagacgt tcacgacgct cggaaccgca gtcacgcggc cgttggagat gttcacgtcg
     4321 acgatgactg cgaacgggcc ctggcgatcg tcggcccgga acacgcggta gccagtggct
     4381 cccgcgaccg ccttccagtc gacctgcacc tctccggaac ccgcccccgg tgccacctcg
     4441 agcgctggcg cgagcagggt cgggggcgcg gcggtggtcg ggctcgatgg cgggatggtt
     4501 gtcgtcgtcg tctcggcagt cgtgtccgtg gtcgaaggac cggtggtcgt ggacgacgtc
     4561 acgacagtcg tcgtagggct ctggttggtc gcgtcgacct tgccgtcatc ctgtcgaagc
     4621 agtgcgacag aagtgaccac aaccgacacg gccaccgccg ctgccaccat ccaccaccgg
     4681 ctcgttctca caagctcagt gtgtgagtga cggacatgtc ctcgtagggg cggaggtccc
     4741 agttcgggaa gaaccagtgc cagcggaaga cgcagatcca cccggcgatc tggaggcggg
     4801 cgagcgcgcc gtgcccgatc gcgctgtggc ggccggggga acggcgcccg agggcgtgac
     4861 ggtcaacccg atcctcgccg acggcggcgc cgcggaggtg ttgctcgagg cggccgtcgg
     4921 agctgacctg ctcgtcgtcg gctcacgcgg tcatggcggc ttcgcaggtc tgctgctcgg
     4981 ctcggtgagc gagcactgcg tgcaccacag cccgtgcccg gtcgtggtcg tgccacacgg
     5041 tcggccgccg cgatagccga ccagtaggag tcaggcgccc ggtcggggaa ccgtcttcac
     5101 gccggcgcgc tccagctcgg cacgcgcgtc gtccggcgga aggctcacca cgaccaccgc
     5161 accccgcgcc gcagcctcga ccgcgatctc ggacagcacc gtgaccacct cggcgtgcat
     5221 gtggacgaca tccatggcgt cgatcacgag ctcgaggttg ccttggccgg cgaccaggtc
     5281 gcccaggatc gtgcgtagca acagcgtgga gtcccggctc agctcgcccg atggcgcgac
     5341 gacgacccga ccgagcgcgc ggtggatcgc gatcgtccgc aagccgtcgt cgggcacgtc
     5401 ccacctccct tggtgcttgc ccggcctgaa acctcgtcac cgtagatcag cggcaacaac
     5461 cgcagctggt cacggccggg tcagcacgac cttcaacgcg ccggtttccg ccgctcgaga
     5521 gaacacgtcg tacgcgtcct cgaactcgtc gaggccgaag tggtgggtga cgaaccgagt
     5581 cgagtcgagc tggcccgtgg cgacgagccg gagcagcgtc ggcgtggtgt acgtgtcgac
     5641 gagtcccgtc gtgatcgtga tgtcgcggat ccactgctcc tcgaggtgga gcgtcgaggg
     5701 ctcgccgtga acgccgatgt tggcgatgtg tcctcccggc cgagccagcg cgaccgcgag
     5761 ctcgaaggct gcgggaacgc cgacggcctc gatcgacaca tcagcaccga gcccgttcgt
     5821 cagaccgagc actgcggcgc gggcgtcctc ccgcccgttg ttcacgacgt tgtcggcgcc
     5881 gaactgcttg gccgcctcga gccggctgtc tgcgaggtcg atcgcgacga cgtggctcgg
     5941 actgaagagt cgggcaccca tcatggcggc gagcccgatc ggcccggctc ccacgactgc
     6001 gacgacgtcg cccggctgga cgcggccgtt cagcaccccg acctcgtagc cggtcggcag
     6061 gatgtccgcc agcatcagga tctggtcgtc ggacacaccg ggcggcacct tgtgggtcga
     6121 ggtgtcggcg aacggcacgc gcacgtactc cgcctgggtc ccgtcgacga ggtgcccgag
     6181 gatccagccg cccccaccga ggcactgtcc gtagcgcccc tcgcgacaga accggcacgc
     6241 gccgcacgac gtgatgcagg acaccaggac tcggtctccg accgcgaccg tcttgacgcc
     6301 cgcgccgacc gcctccaccg tgccgacggc ttcgtggccg aggacccgac cgctggtcac
     6361 cgtggggacg tcgcccttca ggatgtgcag gtcggtccca cagatcgtca ctgcgtcgac
     6421 gcgcacgatc gcgtccgtgt cggcgccgat cgccggatcg ggcacctccg tccacgcctt
     6481 ctgaccaggt ccgtgataca cgagtgcgcg catggtgtcc tccgatccga cgtcttcgat
     6541 cagaccgtac gaatcggaag ggccggcggg cagagtcgaa ggtcacgact cggtccggcc
     6601 gcgcccgggc gccgtgaggg gaccttggcc tctgtcggtg gcgggagcca cggcggacac
     6661 tcgacagtga tgaaggtgcg cgacgtgatg acgaccgagg tggtgacggt tcgaccggag
     6721 acgccgttcg ccgagatcgt gagccagctt ctgtggcagg ggatcagcgg cgtccctgtc
     6781 gtcgacgacg acggccgact gctcggggtt gtcaccgaag cggatctcgt gagcaacgag
     6841 gcgtacggct atcggcgccg ccgggcactc gcgctgatcg gtgactactt gcgcggcttc
     6901 gaccccgggt gggtgcgcaa ggcgtccgcg cggacggccc gtgagctgat gaccgcggcg
     6961 ccagatgtcg ctcgccccga cgagcaggtg gctgacgtgg cccgccggat gctggaagag
     7021 cgacacaagc gactgcccgt cgtggaggac ggccgggtcg tcgggatcgt ctcccggcac
     7081 gacctgcttc gccagttcca ccgccccgac gaggagacag cgggcgacat cgagcgactg
     7141 ctggccgatc cgttccgcgc gcccgagacg catgcggcgg cggcacgggt ggagaacggg
     7201 atagtgacgc tcgagggcac ggtgcagcgg ccgagtgaca agcgggtcgt cgaggccgcc
     7261 gtcgcccaag tgccgggggt ggtcgccgtg gacaatcgcc tcgaagcacg ggagccggag
     7321 ccccgcgcgt tctgacccgg gctgcgaccg gctcgagctc cgccctcggg acgttgtccc
     7381 cttgtgcgcc ggccggcgcg cgcacatcgt caagagcatg acgatgaccg cgccatcacc
     7441 catcgttctg cggcccgacg agctggcgtc cttgccgccg gaggcgctcc tgcctgtacg
     7501 aacagccggt gggctgagcg tccgtgagat cggacgcatg aggacaggag gtgctccatg
     7561 aaggagccac gggtgtggaa cgtcgaggtc gtcttcacag aggacgcgga tcggacccga
     7621 gccgacaccg tgctggacgt cggggacctg cggatccacg ggtgggggcg cgcccggcgc
     7681 cgtcccggcg atcccgacgt gccggcgatc ggcgaggagc tcgccgcggc acgggcgctg
     7741 gcggatctgg cgcaccagct cctccagcac gcggctgact cgatcggcgc gttcgagggc
     7801 catcccgtca acgtccacgg gtgaaggagc gcgggggacc ggcatggcga cggctgcgca
     7861 cgagccggcg aacgggtcgt ctcccgggag cccgtggcgc gccgcggacg cacgaacgca
     7921 gcaccggccg ggcatgtcga tgcgggtggc gaccgtgtcc gggatccaga tccggatcca
     7981 cgcaaccttt ctcgtcctgg tcgccctcgt cgccgtcacc gccaacgcgg aggagggccc
     8041 ggggctgacg tgggggctga tctggctggt cgccctgttc agctgcgtcg tcgtccacga
     8101 gctggcgcac agcctcgtcg cccgacgctt cgggatcccg gtcctcgaga tcgagcttct
     8161 cccgttcgga ggcatctcca agatgggtcg ttcgccgcag gaccctggcg tcgagctgcg
     8221 catcgccctt gccgggccac tggccagcgt gggactggga gcagccttcg catggctcgc
     8281 gctcctcgcc ggcgcgtcga tgtggccgcc gacgctctac ggcggagggt tcctcgcgag
     8341 gctcgcgtgg gtcaacctgc tgctggcggg gttcaacctc gtcccggcgc tgcccctcga
     8401 cgggggccgc gtgctgcgag cgacgctcga acaacggagc gatcgcgacc gggcgacgcg
     8461 agtcgcggca agggccgggc ggttgttcgc tgtcgccatg atcgcggccg ggctcctgtt
     8521 caacctctgg ctgctcgtca tcggcctgtt cgtgtacgcc gggagctggg cggaggaggc
     8581 agccgcactg ctccatgcat gtgtgaagga gctccacgtc agcgacgtga tggtccggga
     8641 tcccatgtgc gtgtcctcgg ccacaccggt cgaggagctg gtcgcggatc tctggcgcac
     8701 ggcccaacgc gagttccccg tcgtcggggc cgacggggcc tgcgaagggg tcgtgaccgc
     8761 ggatgcgctt ctgcgcgccg cgccacatgc tcgtgttggt gcgctcgcgg acgcccgtgc
     8821 ggtcctggcg ccggatgacc cgctggagac gtgcggtctg ctcaccggcg agatcggcgc
     8881 cgccgcggtg gtcagcggcg gacaggtcgt gggtctggcg cgagctgccg acaccgccgc
     8941 cgtcgtgcgc caactgcttc ggcaaggcgc gcaaaagccg tgagtgacca ggagtgaccg
     9001 gtctcgaggg gagcgggcgc tcgccgccgg ggtggcctgc gagacgccgc ggtggacgcg
     9061 gccggtcgcg aagaagggct gacgcgaagg gacgaaggac cctggcgccg cccatgggct
     9121 cggcggatgc tgcccgtcag cgggtcgaac ccacgaccga gggaggcagc catgaagcgg
     9181 ccccgcagat ggtggaacgg acggagccga cgcgcccggt cccaaccggc ctggcggcag
     9241 gccttgatcc cacacggccc ggttcggttc gacacctcga ggtgcagctt ggcggtcgcc
     9301 gtggtcaaca ccgaccgtga gcaacgcact gggtcatgtg gtccgtgcag ggcggcgtga
     9361 gctgagtcgg cgacatggct gcccgaagac gttggagtgc ggcagaccgg gggatggttc
     9421 acctcgtgcg gctggtcacc gcggacgact gggatcccgc cctggcgtcg ctgcggctgc
     9481 gcgaggtcgt cagtgacgcg ctcgtgctgc gccagatgtc tgcgcgggtc cagcgggcga
     9541 tcgcggagcg tccgtcggag gtcgcagaac gcgccgcccg gacgttggcg gcggcgatgg
     9601 gcgtggcgca gtcgacctga cgcgcccggc actcgcgtga ggctgatcgg ccctccggag
     9661 ggccatcgga ctcggacacg atcgaccatt cgcctctgaa cgccgaccga ctcgcggcga
     9721 gatgatccgg acatgatcgg gcgaacgacg gacggctcga acccatgacg tggctcgttc
     9781 tcctggccga gtccgcgata gcggtgtccc tgatcgtgta cctggtccac gaccgccgac
     9841 ttgatgacga cgccgccttc gacgcgtacg tcgacgtcga ggatgtccca cccggcgcag
     9901 acgaccgagc aggaccgccg ctgcatctcg ccgtcgatga ggccgacgac ggtcgcctga
     9961 ctgcggagat cgtcttctgc gaggtgcagc ccggaacctt gcggctggac tgcgtggtca
    10021 gcgcggggga gggtcgcggg tggccgttct cgatcgctct cgagctgccg aacaacgact
    10081 cggccctgtc ggtggcctgg gcgatcggcc agtggatcgc gacgtccgcg gtcgtggtcc
    10141 ttcgcctgcc gcccgaggag gctgaccacc cctacgtcga cctcaccgat ggcatcacga
    10201 ccgtgcgtct cgagacgacc aatcacggtc cgctcctgtg cgagtagggg accgacgtga
    10261 ccttcgcctc tgtgcgccgc cgcggccgtc actgacactg gaggtggacg caagggcgtg
    10321 atggaggtcg tcatggaaca tcaccatgag gtgctgcagg agctgcgaga tccgacgaag
    10381 gccctgcgcc gagccatacc cgagacctgg gcaggcttcg tcgcgctgca cgatgcggcg
    10441 atggccgagg gagaggttcc ggcgcgcctg aaggaggcgg ccgccctcgc gatctcggcc
    10501 gtgaagcgct gcgacggctg catcgcctac cacgccaagg cggcggcaca tgcgggggcc
    10561 acacccggcg aggtggcgga gcttctcggc gtcgcgctgc tcatggacgg cggcaccgcc
    10621 tcggtgtacg cgccgcgtgc gtgggaggcc tatgccgagt tcgtccggga gtacaccggc
    10681 atggaggcgg tcagctgatg ga
//
LOCUS       DATBFZ010000041        15001 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_3965526, whole genome shotgun sequence.
ACCESSION   DATBFZ010000041 DATBFZ010000000
VERSION     DATBFZ010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 15001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15001
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3965526"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="VH419_01215"
     CDS             complement(<1..314)
                     /locus_tag="VH419_01215"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY7042265.1"
                     /translation="MIARPPVPVGIAILGAAHMPHAWSYARAATESAATTLVGVHDVD
                     EAMAQWIRQDFDASYFADVEALLALPDLDAVVICSETATHRRYAELAASAGKHVLCEK
                     PIA"
     gene            complement(311..1309)
                     /locus_tag="VH419_01220"
     CDS             complement(311..1309)
                     /locus_tag="VH419_01220"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY7042266.1"
                     /translation="MENLRVGVVGAGWVAAVHAHAYRGSPGVEITVVTDPVVSKAERL
                     AEQTGARVVPDVQAVIDAGVDVVDVCTPPYVHADVTIQALEAGLHVICEKPLARTLED
                     ARRVAKAAESATGLLMVGHVSRYEPDHVAAKSLVDEGRIGRLRMVTHSTTTSLPGWSE
                     AGWLADPAKSGGPLLDQAVHSFDYFGWLTGCPAVRVTAVGADTGVGPSTYALATVRYA
                     DDTIACAEVSWGHPPARGFKLAAELVGTQGRLTWSYDHMMTGVLYPREGAPEWYDALG
                     DRGFRAELRAFTDAIRAGGPSPVPVADATESLRVALGALESLHTGRTVDLTTWETA"
     gene            complement(1330..2556)
                     /locus_tag="VH419_01225"
     CDS             complement(1330..2556)
                     /locus_tag="VH419_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042267.1"
                     /translation="MTSSAVAPADSTQTRATLDRLLAAGNGVIRLAPAWVARDFLPPG
                     RRLGLPEAAYDVGERGFICERWLASTTKADNRVGPPDEGLSHLVGADGSRLLLRDAVR
                     AAPATVMGSAYAAAHPAGLGRLAKLFDYAYRLPYHVHPPQAFATLVAANAKDEAYHFP
                     AGVDMGAHPESFFGVHPWIAAQRAHDVLLPYLVDWDSDLILRHARAELLVPGEGFHIP
                     SGVLHAPGTALTLELQEDSDVLSMFQALNAGRIISKELLFKDVRPQDREEHGERFPLG
                     FVDWELNGDPYFFENRHLAPVPAGSTEDSTGEQTWIFYNTAKFSGKRLVVGPGQSLTV
                     AEPGVYSVFVWSGQGRYAGHEVRGGDPGLDELLVVHEAAVQPHVVENIGSEDLVVFTF
                     FGPDIQRDVPTIPAFG"
     gene            complement(2560..3552)
                     /locus_tag="VH419_01230"
     CDS             complement(2560..3552)
                     /locus_tag="VH419_01230"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEY7042268.1"
                     /translation="MTAIDHERTDVSEVPVALEMKPPKRDTNKLGRKYSLKSRIATYC
                     ILAPLAILFVAPFAWLVSASFQPITEIFSKTPHWIPANPTMDGYKGFLNVGHLTAAQQ
                     NAGHGDWRWFANSVFVAGTVTILQTFFNALCAYCFAKRQFPGRDVIFVFFLATMMVPG
                     QVTLVPNFIITKHIPFFGGNDWLGNGGSGWLDSCWGLIMPGVVSAFGIFLLRQYMMSI
                     PNELLDAARIDGAGEFRIFWTIVLPLCAPALAATAIFTFQGAWEDFLWPLIIINSPDH
                     YTAPLGLASFVVQNKTSWNLLFAGSVIATLPMIVIFMIFQRRFIQGIALTGVKG"
     gene            complement(3549..4502)
                     /locus_tag="VH419_01235"
     CDS             complement(3549..4502)
                     /locus_tag="VH419_01235"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEY7042269.1"
                     /translation="MTTASVPVRNDKDTRSDIRDSKVRFTLWRMWRERSAYLFIAPGV
                     IIFSVFTVAALIFAFYLTFHQWSIIEPDKPYVGLRNYKDMIHDQGFRHSVFNTAYFTL
                     GSVPVTMAIGLFFALLLNQPIRFLSTFRTMFFLPVVTPFVVSAILWKWLFNGDFGLFN
                     YYLLKVHLINEPLLWLSSQNLAMPSVILMSIWGGVGFSMVIYLAGLQAIPEELYESAR
                     MDGAGAVSRLWYITIPMLAPTTLFLLVIGVISSFQIFTQIYLMTQGGPIDKTTTMVYY
                     IYEWAFKFYDMGYASTLAMGLFLLLLVVTLFQLRLYRRADA"
     gene            complement(4625..5902)
                     /locus_tag="VH419_01240"
     CDS             complement(4625..5902)
                     /locus_tag="VH419_01240"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEY7042270.1"
                     /translation="MAGTSLAACGSDNSDSPESTSTAGQGKGPVPEPTQPVTVTFESW
                     VGGYPDMKKLADQFHKEHPMITIKFINASSDTESQKLTTQIAGNEAPDTAYVNASDTS
                     DFAARGALVNLDDYISRSDIVKPDDYVDAFKTFVEYDGHLWGLPFDGESTGLFYRKDL
                     FEQAGIDHPPTTWDEFLEDAQKLTDPDKDQYGFEMFASESAYYWYPWLYQAGGDLLSD
                     DGQQIVFNDDAGKKAADFYVDLAKYSPPDYYNSNSYDGRVAFENGNVAMYVAGSWLAG
                     TIHSEKPDIDDKWSTAPLPDGDAGCKTTIAGDSLVLFNQSEHPDAAWKWLEFLSKPEN
                     VAKWTYKSPTGTELPPLKSLLNSPDLVQTKPVLEGFAKLMDCGVASSVSQPKFPRVEE
                     ILNEELGKAIYGDQSGSEAVDNAAQKGQLILNR"
     gene            6291..7379
                     /locus_tag="VH419_01245"
     CDS             6291..7379
                     /locus_tag="VH419_01245"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY7042271.1"
                     /translation="MTEVAGQGHTTPATPAAPAGRDGSAAATEPTGQTRVTIAQVADL
                     AGVSPTTVSHVLSGKRVVKATTRGTVQEAIRRLGYRPNNVARSLRTRRSHIVAVVLPD
                     LTNSFYGVLTRGLADAVDQRGYGTYLCNTDGLADRETKFLEDVVDRGADGIVLASVDS
                     ASTAALGPADLGTPVVCVGGSLDHPLIDMVTADDRVGSHAATAHLIARGVTRVAMIQG
                     PATMGTDRVDGFRQAMAAAGLEPDPDLMVEGDWTRQGGYRAMQELLSQPNAPDSVFCA
                     NDLTAIGALEAVRERGLSVPDDVALVGFDDVDAATIVSPALTTVRNPAYDIGRQAGEL
                     LMSRMLGEFKGNGRTVVLPCPLVPRDSA"
     gene            complement(7712..8668)
                     /locus_tag="VH419_01250"
     CDS             complement(7712..8668)
                     /locus_tag="VH419_01250"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEY7042272.1"
                     /translation="MKIAALTKPGHFEIEETPVPTIRPDEVLLRVAACGVCASELDTW
                     KGAGSTDFPLHLGHEVSGTVASIGDDVDGLAVGDPVGVWVTSHGFAEYVAVRAEYCRP
                     AGDVPLDTVLAEPLACATNAVERADVRLGDDVVIIGAGFMGDLVYQLVALRGARRIIV
                     ADTRRDALERSRARGATRAVDVTAESLEDVVRAFTEGRGADVTFECTGTQAALTALGG
                     VARMSGKVVLVGFHQGPPREIPLGYWNWMAFDVVNAHFREVSTIMRGMTVGMRLLAAG
                     RLSMTELVTHRFAIEEIGTAFQTALDKPPGFVKATVLLDPIR"
     gene            complement(8665..10755)
                     /locus_tag="VH419_01255"
     CDS             complement(8665..10755)
                     /locus_tag="VH419_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042273.1"
                     /translation="MQRGVYFDAWFPRQHCYHPSLPPRRLRMVDDLVDYRADVLVWSA
                     MGGGSLALPYLEQEAFGRVEPRSRFYGFVNDSEFVAECHKHQIKVFGIVFEAQGWEFP
                     VELTDDESQILALNELRGVGRRDWIGLREFSQNRYPNLWDPFEKYFPGGLVNSDGEPV
                     TDLIEECVARDIHQQPCHARWVECPDREHQCFYMDRNNPVWREYLKAVIRIQIDAGVD
                     GIQLDEAELPMGAFQYGGCFCKDCMKGFRAYVQDLNEVDPVLDGEDLGSFHYGAWLLE
                     RGYDFKDDREQTPLFGAYYRYQCKAIKRHFGELADYAREYGRERGRDVLVSGNFFNCD
                     PSYLALADDVDLVITEMRNTTYRQPEWYRYVAGFAGDKDVVVVENPYGGVVPEMVDLL
                     AAGRGRDLMRLSLYEGAAFGANMSAPYGSWMGSTIEDSFYAPHDLLVEVQSFLADNDR
                     LYRRQSCHEVAVVYSVESASALVSKADASDNLSNTTDQSVQVPYRVVTRGLADAGVPF
                     DVVLFPDGETAPDRISAPSLDTYRTVVLPDCHHLTPRQADTLLAYVEGGGYVVALGGL
                     GDNLDRGVQQALAGHEGVHRAELGDLEALTPFGPQVEVSVDVAVNVNLLDDGSAAVHL
                     LDYGYDRETDGVPAHEEVSLSVRLPFDVAGATLVSPGSGDSPLPVASDSDAWTVRLPS
                     FRLYAVVVFDAVAP"
     gene            complement(10800..11723)
                     /locus_tag="VH419_01260"
     CDS             complement(10800..11723)
                     /locus_tag="VH419_01260"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEY7042274.1"
                     /translation="MKLGFLTACLPGTPLDEIVDWAATEGYQGLEVAVWPGEPGRPWE
                     ASHIDVRELDTDGAAAVRGLFDRHGLEISALAYYENNLHADEERRRQIHQHLYAVIDA
                     AQRVGCGLVGTFVGRDVTRTVEENLRLGEKVLPPIVEYAAARGVRLVVENCPMEGWHP
                     DGYPANLAYSPELWDWMAGLGLYLNFDPSHLAWLGIDPTAVLPQYADRVLHVQAKDVE
                     IDHAARNRYGLFSRVVDRASDPWASGWWRYRIPGLGDIDWRRLVDQLYEGGFTGFVSV
                     EHEDPVWGGTPEKVLQGLVVARRTLAPLLVA"
     gene            complement(11839..12588)
                     /locus_tag="VH419_01265"
     CDS             complement(11839..12588)
                     /locus_tag="VH419_01265"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEY7042275.1"
                     /translation="MLTERDVEFFRTNGYLLPGRQMFSEEKLGRLEAIFNDHLADKGD
                     KLSDELDTPHYRDERLLEFLLSEEVLDTVEPLIGPNIALWSSHFISKDPFTGRATPWH
                     EDSAYWNGRFSRYDGIVTVWLALENPSNHENGCMRVIPGSHLAGGFSDNYVPTDMSVQ
                     TFHAEIADVDESKAVDFELKRGEFSMHDGRIVHGAKPNTSSMRRTGYTMRYFPTSVRM
                     LPDEINEGWKIWLARGEDLSGNTYANIPAPA"
     gene            complement(12593..13729)
                     /locus_tag="VH419_01270"
     CDS             complement(12593..13729)
                     /locus_tag="VH419_01270"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY7042276.1"
                     /translation="MSKLRVGVIGLGEVAQVIHLPVLESLPDKFEIAAVCDISPSLLA
                     TVGDRYGVPGRYADSADLLRSEQLDGVLVLNSDEYHAECAVNALERGVNVFVEKPMCL
                     SPREAEAIIAARDKSDGAQVMVGYMRRFAPAFLEAKEQLASLGPVNYVRVHDIIGKNQ
                     LIIDQMWNVHRPGDIPEAMVADKWERASKLVQEALGSVPDELAWTYRLLCGLGSHDLS
                     AMRELFGKPKRVLAARQWRSASFVTALFEYDDFVATYETGVDDQVRFDAHIEVYGQSA
                     QLRIQYDTPYVRHFPTTVVVERTNGDALERSVSRPHLKDPYTAELEYFHAMVTEGFAP
                     KTTPEDFVEDLALFAEMIKMMAASAEDGSYVETEHTPTDTDETR"
     gene            complement(13787..14794)
                     /locus_tag="VH419_01275"
     CDS             complement(13787..14794)
                     /locus_tag="VH419_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012921862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEY7042277.1"
                     /translation="MPRPVTLFTGQFADLPFEEMCRMAAEWGYDGLEIACWGDHFDVH
                     RAVTEDDYVQDRRDILDRHGLSVYAISCHLTGQAVCDHPIDERHEGILPTRIWGDGDA
                     EGVRKRAAEEIKDTARAAAALGVDTVIGFTGSSIWHTVAMFPPVPEHMIERGYADFAE
                     RWNPILDVFDEVGVRFAHEVHPSEIAYDYWTTQRAMEAIGHRPAFGLNFDPSHFVWQD
                     LDPVGFLWDFRDRIYHVDCKESVKRFNGRNGRLGSHLPWGDPRRGWDFVSTGHGDVPW
                     EAVFRMLNSIGYAGPISVEWEDAGMDRSVGAPDALAFVRRLTGIEPPTASFDAAFSSG
                     G"
BASE COUNT         2494 a         5032 c         5015 g         2460 t
ORIGIN      
        1 gcgatcggct tctcgcacag cacgtgcttg ccggccgacg cggccaactc ggcgtaccga
       61 cgatgggtgg ccgtctcgct gcagatgacg accgcatcga gatccggcaa ggccagcaag
      121 gcctcgacat cggcgaagta cgacgcgtcg aagtcctgcc gaatccactg ggccatggcc
      181 tcgtcgacgt cgtggacacc gaccagcgtg gtcgcggccg actcggtcgc ggcgcgggcg
      241 tacgaccagg catgcggcat gtgcgcggcc ccgaggatgg cgataccgac gggcacaggc
      301 ggtcgcgcga tcatgccgtc tcccaggtcg tgaggtcgac cgtccggccc gtgtggaggg
      361 actcgagtgc accgagcgcg acccgcaacg actccgtggc atcggcgacc ggaaccggtg
      421 acggcccgcc ggccctgatc gcatcggtga acgcacgcag ctcggcccgg aatcctcggt
      481 caccgagtgc gtcgtaccac tcgggcgcac cctcccgcgg gtacagcacg ccggtcatca
      541 tgtggtcgta gctccacgtc aaccgcccct gcgtgccgac cagttcggct gcgagcttga
      601 agccgcgcgc cggcggatgc ccccagctga cctcggcgca ggcgatcgtg tcgtcggcgt
      661 atcggaccgt ggccagcgcg tacgtcgacg ggccgacccc cgtgtcggcg cccacggcag
      721 tgacccgcac cgccggacag ccggtcagcc aaccgaagta gtcgaacgag tgcaccgcct
      781 ggtcgagcaa cggtccaccg gacttcgcgg ggtccgcgag ccatcccgcc tcgctccacc
      841 caggcagcga ggtcgtggtc gagtgggtca ccatgcggag ccggccgatg cggccctcgt
      901 cgacgagcga cttcgcagcg acgtggtccg gctcgtagcg cgagacatgc ccgaccatca
      961 gcagtccggt ggcggactcc gccgccttcg cgacccggcg ggcgtcctcg agcgtccgcg
     1021 cgagaggctt ctcgcagatg acgtggagtc ccgcttcgag cgcctggatc gtgacatcgg
     1081 cgtgcacgta cggcggcgta cacacgtcga cgacgtccac cccggcgtcg atcaccgcct
     1141 gtacgtccgg cacgactctg gcgccggtct gctcggcgag ccgttctgcc ttcgagacga
     1201 ccgggtcggt cacgacggtg atctccacac ccggcgaccc ccggtacgcg tgcgcgtgta
     1261 cggccgcgac ccagccggcg ccgacgacgc cgacgcgcaa gttctccacg tgcgccttgc
     1321 cttcctctgt caaccgaagg ccgggatcgt cggtacgtcg cgttggatgt ccgggccgaa
     1381 gaacgtgaag acgacgaggt cctcggaacc gatgttctcg accacgtgcg gctgcaccgc
     1441 ggcctcgtgc acgaccaaca gctcgtcgag gccgggatcg ccgccgcgga cctcgtgccc
     1501 ggcgtaccgg ccctgcccgg accagacgaa gacgctgtag acaccgggct cggcgaccgt
     1561 cagcgactgg cccggaccga cgacgagccg cttcccgctg aacttggcgg tgttgtagaa
     1621 gatccacgtc tgctcaccgg tcgagtcctc cgtcgagccg gccggcaccg gcgccaggtg
     1681 ccggttctcg aagaagtacg gatcaccgtt gagctcccag tcgacgaagc cgagcgggaa
     1741 gcgctccccg tgctcctcgc gatcctgcgg gcggacgtcc ttgaacagga gctccttgga
     1801 gatgatgcgc ccggcattca aggcctggaa catggacaag acgtcggagt cctcctgcag
     1861 ttccagcgtc aacgcggtac cgggtgcgtg cagcacaccg gacgggatgt gaaagccctc
     1921 ccccggtacc aacagctcgg cacgggcatg gcgcagaatg aggtcgctgt cccagtcaac
     1981 gaggtagggc agcagcacgt cgtgggcccg ctgtgcggcg atccacgggt gtacgccgaa
     2041 gaacgactca gggtgcgcac ccatgtcgac accggccggg aagtggtaag cctcgtcctt
     2101 cgcgttcgcc gctaccaggg tggcgaacgc ttgcggcgga tggacgtggt agggcagccg
     2161 gtacgcgtag tcgaagagtt tcgcgagccg gcccagcccg gcaggatgcg cggccgcgta
     2221 cgccgacccc atcaccgtcg ccggcgcggc ccggaccgcg tcacgcagca gcagccgaga
     2281 cccgtctgca cccacgagat ggctgagccc ttcgtcaggt gggccgaccc ggttgtccgc
     2341 cttcgtggtc gacgcgagcc accgctcgca gatgaagccg cgctcgccca cgtcgtacgc
     2401 cgcctccggc agccccagcc gacgaccggg agggaggaag tcgcgcgcca cccatgcagg
     2461 cgccaaccgg atcacgccgt tgcccgcggc gaggaggcgg tcgagcgtcg cccgggtctg
     2521 ggtggagtca gcgggagcga cagcagatga cgtcaagcct cagcccttca cgccggtcag
     2581 cgcgatgcct tggatgaacc gccgctggaa gatcatgaag atcacgatca tcggcagcgt
     2641 ggcgatgacc gagccggcga acagcaggtt ccacgacgtc ttgttctgga cgacgaacga
     2701 cgccagcccc agcggggcgg tgtagtggtc gggactgttg atgatgatca gcggccagag
     2761 gaagtcctcc cacgcgccct ggaaggtgaa gatggccgtg gccgccaggg cgggtgcaca
     2821 cagcgggagc acgatggtcc agaagatgcg gaactcgccg gccccgtcga tccgtgcggc
     2881 atccaacagc tcgttcggga tggacatcat gtactgccgc agcaggaaga tgccgaacgc
     2941 actgacgacg cccggcatga tcaggcccca gcaggagtcg agccatcccg acccgccgtt
     3001 gcccagccag tcgttgccgc cgaagaacgg gatgtgcttg gtgatgatga agttggggac
     3061 gagcgtgacc tgtcccggca ccatcatcgt cgccaggaag aacacgaaga tgacgtctct
     3121 cccagggaac tggcgcttcg cgaagcagta cgcgcacaag gcgttgaaga acgtttggag
     3181 aatcgtgacg gtcccggcca cgaacacgct gttggcgaac catcgccagt ccccatgccc
     3241 ggcgttctgc tgggcagcgg tgaggtggcc gacgttcaag aaacccttgt aaccgtccat
     3301 cgtcgggttc gccgggatcc agtgaggggt cttcgagaag atctcggtga tcggctggaa
     3361 ggacgcgctc acgagccagg cgaacggtgc gacgaagagg atcgccagcg gcgcgaggat
     3421 gcagtacgtc gcgatgcggg acttcaggct gtacttgcgg cccagcttgt tcgtgtcacg
     3481 cttcggcggc ttcatctcca gcgcgacggg gacctcactg acgtcggtcc gctcgtggtc
     3541 gatggcggtc atgcgtcggc cctccggtag agccggagct ggaagagggt cacgaccaac
     3601 agcaggagga acaagcccat cgccagcgtg ctggcgtagc ccatgtcata gaacttgaac
     3661 gcccattcgt agatgtagta gaccatcgtc gtcgtcttgt cgatgggccc gccctgcgtc
     3721 atcaggtaga tctgcgtgaa gatctggaac gagctgatca ccccgatcac cagcaggaac
     3781 aacgtggtgg gcgcgagcat cgggatcgtg atgtaccaca gccggctcac ggctccggct
     3841 ccgtccatcc gggctgactc gtagagctcc tcggggatcg cctgcagccc ggcgaggtag
     3901 atcaccatcg agaagccgac gccgccccag atgctcatca ggatcaccga cggcatcgcc
     3961 aggttctggc tggacagcca cagcagtggc tcgttgatca ggtgcacctt gagcaggtag
     4021 tagttgaaga gcccgaagtc accgttgaag agccacttcc acaagatggc cgagacgacg
     4081 aacggggtca cgaccggcag gaagaacatc gtccggaacg tgctcaagaa cctgatcggc
     4141 tggttgagca gcagggcgaa gaagaggccg atcgccatcg tgaccgggac ggatccgagc
     4201 gtgaagtacg ccgtgttgaa gaccgagtgg cggaagccct ggtcgtggat catgtccttg
     4261 tagttgcgca ggccaacgta cggcttgtcg ggctcgatga tgctccactg gtggaacgtg
     4321 aggtagaacg cgaagatcag cgccgccacc gtgaagaccg agaagatgat gacgccgggt
     4381 gcgatgaaca ggtacgccga ccgctcccgc cacatccgcc agagggtgaa ccgcaccttg
     4441 ctgtcgcgga tgtctgatcg ggtgtccttg tcgttccgga cagggaccga cgccgttgtc
     4501 actcacatct ccttgtcggt gctgcgatgc ccggcgtgat cccggacgcc gctcaaggta
     4561 cttcaggcgg ctggctgctg gtccggccaa cgacatcgga ccagcagcca acagccagcg
     4621 gaaactagcg gttgaggatc agttgaccct tctgggctgc gttgtcgacc gcctcggaac
     4681 cgctctggtc gccgtagatg gctttgccga gctcctcgtt gaggatctcc tcgacccgcg
     4741 ggaacttcgg ctgcgagacc gagctggcca ccccgcagtc catcagcttc gcgaagcctt
     4801 cgagcaccgg ctttgtctgc accaggtccg gcgagttgag cagcgacttc agcggcggga
     4861 gctcggtgcc ggtcggggac ttgtaggtcc acttcgcgac gttctccggc ttcgagagaa
     4921 actcgagcca cttccaggcg gcgtccgggt gctccgactg gttgaacagc accaacgagt
     4981 cgccggcgat ggtggtcttg cagcccgcgt cgccgtcggg gagcggtgcg gtcgaccact
     5041 tgtcgtcgat gtccggcttc tcggagtgga tggtgccggc caaccaggag ccggcgacgt
     5101 acatcgcgac gttgccgttc tcgaacgcga cgcgaccgtc gtacgagttg gagttgtagt
     5161 agtccggcgg ggagtacttc gccaggtcga cgtagaagtc ggcggccttc ttgccggcgt
     5221 cgtcgttgaa cacgatctgt tggccgtcgt cgctcaacag gtcgccgccg gcctggtaga
     5281 gccacgggta ccagtagtac gccgactccg acgcgaacat ctcgaagccg tactggtcct
     5341 tgtcgggatc ggtgagcttc tgcgcgtcct cgaggaactc gtcccaggtc gtcggcgggt
     5401 ggtcgatgcc ggcctgttcg aacaagtcct tgcggtagaa cagcccggtt gactcgccgt
     5461 cgaacggcag cccccacagg tggccgtcgt actcgacgaa cgtcttgaac gcgtcgacgt
     5521 agtcgtcagg cttcacgatg tcgctgcggc tgatgtagtc gtcgaggttg accagggcgc
     5581 cgcgagccgc gaagtccgac gtgtcgctgg cgttgacgta cgccgtgtcg ggcgcctcgt
     5641 tgccggcgat ctgcgtggtg agcttctggc tctcggtgtc ggagctcgcg ttgatgaact
     5701 tgatcgtgat catcgggtgc tctttgtgga actggtcggc gagcttcttc atgtcggggt
     5761 agccgccgac ccatgactcg aacgtgaccg tgaccggctg ggtcggctcg ggcaccgggc
     5821 ccttgccctg tcctgccgtc gacgtgctct ccggcgagtc ggagttgtcg cttccgcagg
     5881 cggccagact cgtgccggcc acgacgacga ccgccgcgag aaccggtgtc ctccaagact
     5941 taggcatttt cacgcaacac agctcccttg ctcgttcccc gaaccgatga cggaccagcc
     6001 ttcgcacgcc ccgatgcaaa tcgtttggcg ccctgttcgg gaagcatgat cgggggcggc
     6061 ttgcgttgtc aagggatcgc ggagaagtgc cgagccgcgg tgatcaacgt gtgacgggtc
     6121 ggtgggcagg ttcgacatgg acatcggcgc gaagcgtacc ccgggctggc agccgagcca
     6181 ctttcggcgc atgaccaaat cgttttgggt ccgagttgct accatacgaa cgctgttcgg
     6241 gccgtcgacg acggcgggtg gcgaggccga ggagatcgca aggggagccg atgaccgaag
     6301 tagccggcca gggacacacc acgcccgcga cacccgcggc gcccgctgga cgggacgggt
     6361 cagccgctgc gaccgagccg accggacaga cccgggtcac gatcgcgcag gtcgccgacc
     6421 tggccggagt cagccccacc accgtgtcgc acgtcctgtc cggcaagcgt gtcgtcaagg
     6481 cgaccacgcg cggcaccgtg caggaggcca tccgccggct gggctaccgc ccgaacaacg
     6541 ttgccagaag tctgcggacc aggcgatcac acatcgtcgc cgtcgttctg ccggatctca
     6601 cgaactcgtt ctacggcgtc ctcacccggg ggctggccga cgcagtcgac cagcggggct
     6661 acgggacata tctgtgcaac accgatgggc tggccgaccg agagacgaag ttcctcgagg
     6721 acgtcgtcga tcgaggcgct gacggcatcg tgctcgcgtc tgtcgacagc gcatcgaccg
     6781 ccgccctcgg ccccgccgac ctgggtacgc cggtcgtgtg cgtcggcggc agcctcgacc
     6841 accccctcat cgacatggtc accgcagacg accgcgtggg gtcgcacgcg gccacggcgc
     6901 acctcatcgc tcgtggcgtc acgcgtgtcg cgatgatcca gggaccggcc acgatgggca
     6961 ccgaccgggt cgacggcttc cggcaggcca tggcggcggc aggactcgaa cctgaccctg
     7021 acctgatggt ggagggcgac tggacgcgtc agggcggcta tcgcgcgatg caggaactgc
     7081 tgtcacagcc gaacgcgccg gacagcgtgt tctgcgccaa cgacctcacc gcgatcgggg
     7141 ccctggaggc cgtccgcgag cgaggactct cggtcccgga cgacgtcgcc ctggtcggct
     7201 tcgacgacgt cgacgccgcg acgatcgtgt cgcccgcgct gacgacggtt cgcaacccgg
     7261 cgtacgacat cgggcgtcag gcaggcgaac tgctgatgag ccggatgctc ggcgagttca
     7321 agggcaacgg tcgaaccgtg gtcctcccct gtccgctcgt cccccgcgac tccgcctagc
     7381 ccaccctccg ccgaggtggc aagtttcgtt cggttttgtc ggcgtgtcgc gcactgattg
     7441 ccacctcgat caggtcattt gccgaggact gcggcggtcg agcctgtcga gaccccgacc
     7501 ccttgatcgt gatccttgat ctcgacaggt ggttcctgag cttgtcgaag ggctcgatca
     7561 gcgagctccg gcggtcgagc ctgtcgagac cccgacccct tgatcgtgat ccttgatctc
     7621 gacaagctcg atcagcggac ttcggccctt gatcccgaga ggtggttcct gagcttgtcg
     7681 agaccccgac cctcgatctc gacaggctcg atcagcggat cgggtcgagg aggacggtgg
     7741 ccttgacgaa gccgggcggc ttgtcgaggg cggtctggaa cgccgtaccg atctcctcga
     7801 tcgcgaaccg gtgggtgacc agctccgtca tcgacagcct gcccgctgcg agcagccgca
     7861 tgcccacggt catgccccgc atgatggtgg agacctcgcg gaagtgcgcg ttgacgacgt
     7921 cgaacgccat ccagttccag tagccgagcg ggatctcccg tggcggaccc tggtggaagc
     7981 cgaccagcac gaccttcccg ctcatccggg cgacaccgcc gagggcggtc aacgcggcct
     8041 gggttccggt gcactcgaac gtcacgtccg cgccacgacc ttcggtgaag gcgcgtacga
     8101 cgtcctcgag cgactcggcc gtcacgtcga cggcgcgggt tgcgccacgt gcccgcgaac
     8161 gctcgagggc gtccctgcgt gtgtccgcca cgatgatccg ccgggcaccg cgaagggcca
     8221 ccagctggta caccaggtcg cccatgaagc ccgccccgat gatgacgacg tcgtcgccga
     8281 gccggacgtc ggcgcgttcg acggcgtttg tagcgcaggc cagcggttcg gcaagcaccg
     8341 tgtcgagcgg tacgtcgccc gccggtcggc agtattccgc ccggaccgcg acatactccg
     8401 cgaaaccatg cgacgtgacc cagacgccga ccgggtcgcc caccgccagg ccgtcgacgt
     8461 cgtcaccgat cgacgccacc gtgccactca cctcgtgtcc gaggtggagc gggaagtcgg
     8521 tggacccggc gcccttccag gtgtccagct cggacgcgca cacgccgcaa gccgccaccc
     8581 ggagcagcac ctcgtccggt cggatcgtcg ggaccggagt ctcctcgatc tcgaagtggc
     8641 cgggcttggt cagcgcggcg atcttcatgg tgccaccgcg tcgaagacca cgacggcgta
     8701 caggcggaac gacggaagcc ggaccgtcca tgcgtcgctg tcggaggcca cgggcagcgg
     8761 gctgtccccg gacccgggac tgaccagcgt cgcgcccgcc acgtcgaatg gcagccgcac
     8821 ggacagcgag acctcctcat gggcgggtac gccgtccgtc tcccggtcgt agccgtagtc
     8881 gagcaggtgg accgcggccg agccgtcgtc gagcaggttc acgttcaccg cgacgtcgac
     8941 ggagacctcc acctgcggtc cgaacggcgt cagcgcctcg aggtcgccga gctccgcccg
     9001 gtggacgcct tcgtggccgg ccagtgcctg ttgcacccca cgatcgaggt tgtctcccaa
     9061 cccgccgagt gcgacgacgt atccaccgcc ctcgacgtaa gccagcaagg tgtcggcctg
     9121 gcggggagtc aggtggtgac agtccggaag caccaccgtg cgatacgtgt ccagcgaggg
     9181 tgccgagatc cggtctggcg ccgtctcgcc gtccgggaac aacaccacgt cgaacggcac
     9241 accagcatca gcaagcccgc gggtcacgac ccggtagggc acctgcacgg actggtccgt
     9301 cgtgttgctc aggttgtcac tggcgtcggc cttgctgacg agtgcgctcg cgctctccac
     9361 gctgtagacg accgccactt catgacagct ctgacgacgg tagagccggt cattgtcggc
     9421 gaggaagctc tgtacctcga cgagcagatc atgcggcgcg tagaacgagt cctcgatcgt
     9481 gctgcccatc caggacccgt atggcgcgct catgttggca ccgaacgcgg cgccttcgta
     9541 cagcgacagc cgcatcagat cgcgaccacg tccggctgcg agcaggtcga ccatttcggg
     9601 aaccacgccg ccgtaggggt tctcgacgac gaccacgtcc ttgtcgccgg cgaacccggc
     9661 gacgtaccgg taccactccg gctgccggta cgtcgtgttg cgcatctcgg tgatgacgag
     9721 gtccacgtcg tcggcgaggg cgagatagct cgggtcacag ttgaagaagt tgcccgacac
     9781 gagcacgtcg cgcccccgct cgcgaccgta ctcacgcgcg tagtccgcca gctcgccgaa
     9841 gtgacgtttg atcgccttgc actggtagcg gtagtacgcg ccgaacagtg gcgtctgctc
     9901 acggtcgtcc ttgaagtcgt agccgcgctc gagcagccac gcaccgtagt ggaacgaccc
     9961 gaggtcctcg ccgtcgagca ccgggtcgac ctcgttcagg tcctgcacgt acgcccggaa
    10021 gcccttcatg cagtccttgc agaagcagcc gccgtactgg aaggcaccca tgggcaactc
    10081 ggcttcgtcg agctggatgc cgtcgacgcc ggcatcgatc tggatcctga tcacggcctt
    10141 gaggtactcc cgccagaccg ggttgttgcg gtccatgtag aagcactggt gctcccggtc
    10201 cgggcactcg acccagcggg catggcacgg ctgttggtgg atgtcgcggg cgacgcactc
    10261 ctcgatcagg tcggtgaccg gctcgccgtc gctgttgacc aggccgcccg ggaagtactt
    10321 ctcgaacgga tcccacaggt tcgggtagcg gttctgcgag aactcccgta gtccgatcca
    10381 atctcgtcgg cccactcccc tgagctcgtt gagtgccagg atctgcgact catcgtcggt
    10441 cagctcgacc gggaactccc agccctgcgc ctcgaacacg atgccgaaca ccttgatctg
    10501 gtgcttgtgg cactcggcca cgaactcgct gtcgttgacg aagccataga agcgggaccg
    10561 cggctcgacc cggccgaagg cctcctgctc gaggtacggc agcgcgagcg aaccgccacc
    10621 catcgccgac cacaccagca cgtccgcgcg gtagtcgacc aggtcgtcga ccatgcgcag
    10681 ccgacgtggc ggcaggctcg ggtgatagca gtgctgacgg gggaaccagg cgtcgaagta
    10741 gacaccgcgc tgcatggccg agcctttctg gcttgcgagg tctttcgtga cgcagggcgc
    10801 tacgccacga gcagtggagc gagcgtacgc cgcgcgacca ccaggccctg cagcaccttc
    10861 tccggcgtcc cgccccagac aggatcctcg tgctcgaccg acacgaagcc ggtgaagcca
    10921 ccttcgtaga gctggtccac gaggcggcgc cagtcgatgt caccgaggcc ggggatccgg
    10981 tatcgccacc agccgctcgc ccacgggtcg gaggcgcggt cgacaaccct gctgaacagg
    11041 ccgtagcggt tcctggctgc gtggtcgatc tccacgtcct tggcctggac gtggaggacc
    11101 cggtcggcgt actgcggcag caccgctgtc gggtcgatgc caagccatgc caggtgcgac
    11161 gggtcgaagt tcaggtacaa cccgagaccc gccatccagt cccacagctc gggcgagtac
    11221 gcgaggttcg ccgggtagcc gtcggggtgc cagccctcca tcggacagtt ctcgaccacg
    11281 aggcggacgc cgcgcgcggc ggcgtactcc acgatcggcg gcaacacctt ctcgccgagt
    11341 cgcaggttct cctccacggt gcgggtcacg tcgcgaccga cgaaggtgcc gacgagaccg
    11401 cagccgactc gttgcgccgc atcgatcacg gcgtacaggt gctggtggat ctgccggcgc
    11461 cgctcctcgt cggcgtgcag gttgttctcg tagtacgcca gcgcggagat ctccaggccg
    11521 tgccggtcga acagaccccg tacggcggcc gcgccgtccg tgtccagttc gcgtacgtcg
    11581 atgtggctgg cctcccacgg ccgaccgggc tcgcccggcc agaccgccac ctcgaggccc
    11641 tgatagccct cggtggcggc ccagtcgacg atctcgtcca gcggcgtacc ggggaggcag
    11701 gccgtcagga agccgagctt caccggttca cccaggccga cgcccaactg ccgtggcgat
    11761 cgaggtggcg ttcgaggtgg caatcgacgt tcgacacgcc gtcagaatcc aacaaaagct
    11821 gccacctcgg cgagggggtc atgcgggggc ggggatgttg gcgtaggtgt tgccggacag
    11881 gtcctcgccg cgggcgagcc agatcttcca gccttcgttg atctcgtcgg gcagcatccg
    11941 gaccgacgtc gggaagtaac gcatggtgta accggtccgg cgcatgctgc tcgtgttcgg
    12001 cttggcgccg tgcacgatcc ggccgtcgtg catcgagaac tcgccgcgct tcagctcgaa
    12061 gtcgacggcc ttgctctcgt ccacgtcggc gatctcggcg tggaacgtct ggacgctcat
    12121 gtcggtcggg acgtagttgt cggagaagcc gcccgcgagg tggctccccg ggatgacccg
    12181 catgcagccg ttctcgtggt tgctcgggtt ctccagggcc agccagacgg tgacgatgcc
    12241 gtcgtaccgg ctgaaccgac cgttccagta cgcgctgtcc tcgtgccacg gcgtggcgcg
    12301 gccggtgaac gggtccttcg agatgaagtg gctcgaccag agcgcgatgt tcggaccgat
    12361 cagcggctcg accgtgtcga gcacctcctc ggacagcagg aactccagca gccgttcgtc
    12421 gcggtagtgc ggcgtatcga gctcgtcgga gagcttgtcg cccttgtcgg ccaggtggtc
    12481 gttgaatatg gcctcgagcc gtcccagctt ctcctccgag aacatctgcc tgccgggaag
    12541 caggtaaccg ttcgtccgga agaactccac atcgcgctcg gtcagcacga cttcacctcg
    12601 tctcatctgt gtctgttggg gtgtgttcgg tctcaacgta cgacccgtcc tcagccgagg
    12661 cggccatcat cttgatcatc tccgcgaaca gcgcgagatc ctccacgaag tcctccggcg
    12721 tcgtcttcgg cgcgaagccc tcggtgacca tggcgtggaa gtactcgagc tcggcggtgt
    12781 acgggtcctt caggtgcgga cgactcacgg accgctcgag cgcgtcgccg ttcgtgcgct
    12841 ccacgaccac ggtggtcggg aagtgccgca catagggcgt gtcgtactgg atacgcagct
    12901 gcgccgactg cccgtacacc tcgatgtggg cgtcgaagcg gacctggtcg tcgacacccg
    12961 tctcgtacgt cgccacgaag tcgtcgtact cgaagagcgc cgtgacgaag ctcgcactgc
    13021 gccactgacg cgccgccagg acccgcttcg gcttgccgaa gagctcacgc atcgcggaga
    13081 ggtcgtggct gccgagaccg cagagcagtc ggtaggtcca ggcgagctcg tccggcaccg
    13141 atccgagcgc ctcctggacg agcttgctcg cgcgttccca cttgtccgcg accatggcct
    13201 cggggatgtc gccaggccgg tgcacgttcc acatctggtc gatgatcagc tggttcttgc
    13261 cgatgatgtc gtgcacgcgg acgtagttga ccgggccgag tgacgcgagc tgctccttcg
    13321 cctccaggaa ggccggagcg aaacgccgca tgtagcccac catcacctgg gctccgtcgg
    13381 acttgtcccg ggcggcgatg atcgcctccg cctcccgagg gctgaggcac atcggcttct
    13441 cgacgaagac gttgacgcca cgctcgagtg cgttgaccgc acattcggcg tggtactcgt
    13501 cgctgttgag gacgagtact ccgtcgagct gctcggacct gagcaggtcg gccgagtcgg
    13561 cgtaccggcc gggtacgccg taccggtcgc ccaccgtggc gagcagggac ggcgagatgt
    13621 cgcagacagc ggcgatctcg aacttgtccg gcaacgactc gagcacgggc aggtggatga
    13681 cctgcgccac ctcgccaagt ccgatcacgc cgactcgtag cttgctcacc cgtagaacct
    13741 tccttgcatc acttgaaccc ggagatgtgg tgggacgagc cgtcgctcag ccgcccgagg
    13801 agaacgccgc gtcgaacgat gccgttggcg gctcgatccc ggtcagccgg cgtacgaacg
    13861 cgagtgcgtc cggcgcgccc accgagcggt ccatgccggc gtcctcccac tcgaccgaga
    13921 tggggcccgc gtagccgatc gagttgagca tccggaacac cgcctcccac ggcacgtcac
    13981 cgtgtccggt cgacacgaag tcccagccgc gccgcgggtc gccccagggc aggtgcgacc
    14041 cgagacgccc gttgcggccg ttgaagcgct tgaccgactc cttgcagtcg acgtggtaga
    14101 tgcggtcccg gaagtcccag aggaacccga ccgggtccag gtcctgccag acgaagtggc
    14161 tcgggtcgaa gttgagaccg aacgccggcc ggtgcccgat cgcctccatc gcgcgctggg
    14221 tggtccagta gtcgtaggcg atctcgctcg ggtggacctc gtgcgcgaac cggacgccga
    14281 cctcgtcgaa gacgtcgagg atggggttcc accgttcggc gaagtcggcg tacccgcgtt
    14341 cgatcatatg ctccggcacc ggcgggaaca tcgccaccgt gtgccagatc gacgagccgg
    14401 tgaacccgat gacggtgtcg acgccgagcg cggccgcggc ccgggcggtg tccttgatct
    14461 cctccgcagc gcgcttccgg acgccctcgg cgtcgccgtc gccccagatg cgggtcggca
    14521 ggatcccctc gtgccgctcg tcgatggggt ggtcacagac cgcctggccg gtgaggtggc
    14581 aagagatcgc gtacaccgag aggccatgcc ggtcgaggat gtcgcggcgg tcttggacgt
    14641 agtcgtcctc ggtcaccgcg cgatgcacgt cgaagtggtc gccccagcag gcgatctcga
    14701 gcccgtcgta cccccactcg gccgccatcc ggcacatctc ctcgaacggg agatcggcga
    14761 actggccggt gaacaaggtg actggtcgtg gcatccgagg cctcctgctg tcgtgctgcg
    14821 cgtcgtgggg gctgtacgtc gtcagtgcgt cgggagcgag atccagcgcg agccgtcgtt
    14881 cgaggagcgt tcgacggcgt cgagcacccg ctgcaccatc aggccctcct cgaacgacgg
    14941 cgtgggggga cgaccggccg ccacgtcgct gacgaagtcg gcgacctcgt gcacgaaggt
    15001 g
//
LOCUS       DATBFZ010000042        10921 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_2652834, whole genome shotgun sequence.
ACCESSION   DATBFZ010000042 DATBFZ010000000
VERSION     DATBFZ010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 10921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10921
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2652834"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1530
                     /locus_tag="VH419_01280"
     CDS             <1..1530
                     /locus_tag="VH419_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011753811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEY7042278.1"
                     /translation="QAPEEADLGFPWWLQVTHFINFLFIGLLIRSGWEILASHPRLYW
                     RNDCGPGSEWLRFTKDKVPTEVGAFTARDDQRTLHPLISLPGRARIGIGRAWHGIVTT
                     LWILNGLVYVVLLFATGQWRRLIPTSWDVFPDAWESLRIYAGFGVPSIEHFQPYDALQ
                     QLMYSFIVFVLAPLMILTGPVMSPAVVGRFPWWAKIFGGRQAARSIHFIGMVIFVVFV
                     VMHVTLVFVVHPEHNLVHMMMGTEYDPALVAQAVTRVIIGVVVVVAIWIAASYWSLRD
                     LRTSQRVLYGVQMPVKRLFLDRLTSHQRRKQVFTEKDISPFHWVNTRPPDLDQSPEWV
                     ALRQDDFRDFRLEVGGLVSHARSFSLDELEALASQDQITLHTCMQGWTGIAKWSGIPL
                     REVLAQVEPLEDANYVLIESFGMAQQMHDGRPVEPYYTVLTQAMAMEDETILAWGMNG
                     APLPDMYGAPLRLRAESMHGYKMVKWVRSVSWIRDYAEVGDGMGGTREDSGYQDMDAR
                     I"
     gene            1601..2356
                     /locus_tag="VH419_01285"
     CDS             1601..2356
                     /locus_tag="VH419_01285"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY7042279.1"
                     /translation="MAGQRAASVYADFLLPHLTPTSRLVDLGCGDGELTLDLASSVGH
                     VTGVDVDQDELDRASRAADELGVTNATFILGDVGALDIPDDDVDVVLAHSVLEALEHP
                     DAAVAEMARIVRPGGLVAVASVDYGGLVLAGPQVDVVRRFYAVREQLWLSEGADPYLG
                     RRLRGLLLGAGLHDVVASSTYICHGTARAVRQFGQGRAEDCQDDWYVDSALRHGLATG
                     KEIEEMRRAWLEWSQAPTSYAAFCWCRALGWKG"
     gene            2457..3122
                     /locus_tag="VH419_01290"
     CDS             2457..3122
                     /locus_tag="VH419_01290"
                     /inference="COORDINATES: protein motif:HMM:NF014089.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HEY7042280.1"
                     /translation="MRSELFGTALESATTDRFTLQNARLLRVALDGEVMARQGAMIAY
                     QGEVDFAYQGSGGMGKLFKKAFTGEGMSLMKVSGKGDVFFADDAMEIHLVNLENDSLT
                     VNGSNVLAFDSTLTWDIKRVEGASMMAGGVFNTTFTGVGSIAITAHGTPVVLNVDAPT
                     YADVQSAVAWSTSLSTTVRRTAGAGALIGRGSGEAFQLSFSGEGFVIVQASEGRIVVP
                     HSH"
     gene            complement(3154..3708)
                     /locus_tag="VH419_01295"
     CDS             complement(3154..3708)
                     /locus_tag="VH419_01295"
                     /inference="COORDINATES: protein motif:HMM:NF019500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HEY7042281.1"
                     /translation="MREMSYDSLTRAGRRRAMARPLLRALIVTVAVFVLYFTLPFDKS
                     SAFSTALALSLGLVAIACLLAWQTHAIARSPYPRVRAIESLATSFPLVVLLFSTTYFL
                     MDQYDETSNFSEPMTRIDALYFTVTTFATVGYGDITPVSETARIIAVVQMVVDLILIG
                     LVVHVFLGSVRAGLARRDAGPDDV"
     gene            complement(3893..4438)
                     /locus_tag="VH419_01300"
     CDS             complement(3893..4438)
                     /locus_tag="VH419_01300"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY7042282.1"
                     /translation="MSLSLIALSAESIRRVYAGESDWAAGQPDLSGVVWPADDRRVLR
                     YRVEALDSDPEAAPFLLHAAVQDGGFVGRIGSHAAPDAGGAVEIGYAVVPSARGQGVG
                     GQIVDLFLTWLSARGVTRVDASVGPDNVPSLRLLKRRGFVEVGERWDDEDGRELVLSK
                     ALEPRPRSSDDTPTATEPPAT"
     gene            complement(4435..6084)
                     /locus_tag="VH419_01305"
     CDS             complement(4435..6084)
                     /locus_tag="VH419_01305"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007078475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEY7042283.1"
                     /translation="MNVPIELRRRTRAWIADDPDEETRAELADLLSLADGGHPAAMAD
                     LIDRFDGRLEFGTAGLRGALAAGPNRMNRVVVTAAAAGLAAYLRETAGSGTVVIGYDA
                     RHKSEVFARDTAEVMCGAGLDAVVMPRPLPTPLVAFAIRHLGCVAGVMVTASHNPPQD
                     NGYKVYLGDGSQIVPPADADISAKIDAVEAVAEVPRSAGWTVLGEDIVESYIAAVAAL
                     PASDSARDLTMVYTPLHGVGDETVEKVLVRAGFTAPIVVPEQAVPDADFPTVSFPNPE
                     EPGAIDLAMALAAENDADIVVANDPDADRCAVAVSTDHGWQMLRGDEVGALLADHLLR
                     RGVRGVFATTIVSSSLLGEQARAAGVDFAETLTGFKWIGRVPGLAFGYEEALGYCAAP
                     DLVRDKDGVSALVRVAELAAELKASGKSLLDRLDEIAARFGLYATDQLSVRVDDVTRI
                     GEALERLRETPPSQLGGYDVVQTDDLNEGTDSLPPTEGIRYRLAEGGRVVVRPSGTEP
                     KLKCYLEVVVPVDDSDVEAARISAAGRLDAIRGDLAAAAGL"
     gene            complement(6134..6952)
                     /locus_tag="VH419_01310"
     CDS             complement(6134..6952)
                     /locus_tag="VH419_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY7042284.1"
                     /translation="MRVLVTGAAGHIGSVLSDRLSARHQMRGLDLAAWHAPEDCGAAY
                     PGEFVVGDCADPAVAAAAVDGMDAVVHLAGVASEAALPDILHGHVVGTAAILDAMVQH
                     GVRRMVFASSNHAVGMTPRPDSGPIGVDTPARPDTFYGVGKVAGEALLSLYADRYGVS
                     SVAMRIGSFRQRPDSSRHLSTWLSYDDCTAMVDAALTADFEGFRVVYGISANRDAWWD
                     LGPGRALGFEPRDDAAIVAPELVTSRAPAIAITRVGGEFTTAEFDRRPFDVTAT"
     gene            complement(6952..7773)
                     /locus_tag="VH419_01315"
     CDS             complement(6952..7773)
                     /locus_tag="VH419_01315"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEY7042285.1"
                     /translation="MSDPVPDVTTPSPYDVADQAAKRLGELTGLARHDVALVMGSGWL
                     PAADALGATGAEISTTDLPGFAPPAVEGHAGKIRSVDASGVRALVFLGRTHFYEGLGV
                     AAVVHGVRMAAAAGCRVVVLTNGCGGVRPEWSPGTPVLIRDHINLTGSSPIVGARFID
                     LTDLYSSRLRALCRDADPTLDEGVYVQLPGPHYETPAEIAMVRTIGGDLVGMSTTLEA
                     IAAREAGMEVLGISLVTNAAAGLTGAPLNHDEVLEAGRQAATRMGDLLAAIVPRV"
     gene            complement(7795..8238)
                     /locus_tag="VH419_01320"
     CDS             complement(7795..8238)
                     /locus_tag="VH419_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017934687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HEY7042286.1"
                     /translation="MVLYAAYGSNMDPARMAERCPRSPTRGSGWLSGWRLTFGGEEHG
                     WDGALATVVEAPGEQVFVALYELAQEDERHLDEWESSVYGKIRVRVSTLDGEQLAWLY
                     VLDAYEGGLPSASYLGVLSEAAQAAGAPDDYVHDLRTRPCRSVSE"
     gene            8277..9665
                     /locus_tag="VH419_01325"
     CDS             8277..9665
                     /locus_tag="VH419_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002516527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone dehydrogenase"
                     /protein_id="HEY7042287.1"
                     /translation="MTRVVIIGGGPGGYEAALVGAQLGAEVTIVDRDGIGGSAVLTDC
                     VPSKTLVATSELMTSVQESGELGVRLAGQPPDAGHSASVDLAVVNERVRKLAAQQSAD
                     ITRRLDQEDVRIIRGTGRLDGPDTVVAATADSEVRLEADAVLVATGAHPRVLPSAEPD
                     GERILTWEQVYDVDELPARLVVIGSGVTGAEFASAYNALGVDVVLVSSRDRVLPGEDA
                     QAAEVLEDVLKRRGMTVLGRSRAADVVREGDSVAVTLTDGSSVEGSHCLLAVGSIPNT
                     AGLGLEEAGVTLGDGGFVTVDRVSRTTGRGVYAAGDCTGVLMLASVAAMQGRIAMWHA
                     LGDAVSPLDLNTVSSTVFTSPEIATVGYSQQAVDAGVIEANQQMLPLAGNARAKMQGV
                     HDGFVKLFCRPGTGIVVGGVVVAPRASELIHAVSLSVEASLTVDELAHAFTVYPSMSG
                     SIAEAARRLHTR"
     gene            complement(9720..10754)
                     /locus_tag="VH419_01330"
     CDS             complement(9720..10754)
                     /locus_tag="VH419_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042288.1"
                     /translation="MDESVLLRDALQAGRTRSQMRGPGWTPISHGLYVRREADADLID
                     RCRHVRPILPWDAACSHLTAARLWSTWLPALPDWLPLQVTIPPGAQRPERAGLYVARS
                     RAALPPPIVVSGVPVLDPALVIGQLAEDLQLIDLVIAIDGFLQQQLCTEDDTWQAIRS
                     RQRGLPMLRRALALVDGRSESRWESVLRLFHVLCKIPVEAQYPIRDADGEVVARADLR
                     IKETRRLPEYDGANHRDRERHRRDLARDKLLARSNWERYGYTSVEILDNPTQILRDAE
                     NALGLRHDRTRLEPWLTEVERSSLSQLGYRRLLRRLHRFAKPLRGRGERRLRAAAECG
                     KVDPDKGVSA"
BASE COUNT         1750 a         3678 c         3762 g         1731 t
ORIGIN      
        1 caggctcctg aggaggcgga cctcggtttc ccgtggtggc tgcaagtcac ccacttcatc
       61 aacttcctgt tcatcgggct cctcatccgc tccggctggg agattctcgc ctcgcatccg
      121 cgcctgtact ggaggaacga ctgcgggccg ggctcggagt ggctgaggtt caccaaggac
      181 aaggtgccaa ccgaggtcgg tgcgttcacc gcccgcgacg accagcgcac cctgcacccg
      241 ctgatctcgc tgccgggacg ggccaggatc ggaatcggtc gcgcctggca cggcatcgtg
      301 acgacgctgt ggatcctcaa cggactggtc tacgtcgtac tgctgttcgc gaccgggcag
      361 tggcggcgcc tgatccccac ctcctgggac gtctttcccg acgcctggga atcgctgagg
      421 atctacgcgg gattcggtgt gccgtcgatc gagcacttcc agccgtacga cgcgctgcaa
      481 cagctcatgt actcgttcat cgtgttcgtc ctcgcgccgc tcatgatcct caccggcccg
      541 gtgatgtcgc cggcggtcgt cggcaggttc ccctggtggg cgaagatctt cgggggacgg
      601 caggccgcgc gatcgatcca cttcatcggc atggtgatct tcgtcgtctt cgtcgtgatg
      661 cacgtgacgc tggttttcgt cgtgcacccc gagcacaacc tggtccacat gatgatgggc
      721 accgagtacg accccgcgtt ggtggcccaa gccgtgaccc gggtgatcat cggtgtcgtc
      781 gtggtcgtcg cgatctggat cgccgcctcg tactggtcgc tgcgtgacct gcgcacgagc
      841 cagcgcgtcc tgtacggcgt gcagatgccc gtcaagcgtc tcttcctcga ccggctcacc
      901 tcgcaccagc gccgcaagca ggtcttcacc gagaaggaca tctcgccgtt ccattgggtc
      961 aacacccggc cgcctgacct cgaccagtcg ccggagtggg tggcgttgcg ccaggacgac
     1021 ttccgggact tccggctgga ggtgggcggc ctggtcagcc acgcgaggtc cttctccctc
     1081 gatgagctcg aggcgttggc aagccaggac cagatcaccc tgcacacgtg catgcaggga
     1141 tggacgggaa tcgccaagtg gtcgggcatc ccccttcgcg aggtcctggc ccaggtcgag
     1201 ccgctcgagg acgccaacta cgtgctgatc gagtcgttcg ggatggccca gcagatgcac
     1261 gacggccggc cggtggagcc gtactacacc gtgctgaccc aggcgatggc catggaggac
     1321 gagacgatcc tggcgtgggg catgaacggc gcgccgctgc ccgacatgta cggggcaccg
     1381 ctgcgactac gcgctgagtc catgcacggc tacaagatgg tcaagtgggt tcggtcggtg
     1441 tcgtggatcc gtgactacgc cgaggtcggg gacggcatgg gcggcacccg ggaggactcc
     1501 ggctaccagg acatggacgc gcggatctga tcggccgagg gcgagtgcaa gcgatgacgg
     1561 acgatcaacg tcttcatcgc ctcgtgtggg agcctgggtc atggccggcc agcgtgcggc
     1621 gtcggtgtat gccgacttcc tgctcccgca cctgaccccg acgtcacggc ttgtcgatct
     1681 cgggtgcggt gacggtgagc tgaccctcga cctggccagc agcgtcgggc acgtgaccgg
     1741 cgtggacgtg gatcaggacg agctcgaccg ggccagccgc gctgccgacg agctcggggt
     1801 cacgaacgca accttcatcc tcggcgacgt gggtgcgctc gacatcccgg atgacgacgt
     1861 cgacgtcgtc ctggcgcact cggtgctcga agctctggag catcccgatg ctgcggtcgc
     1921 ggagatggca cgcatcgtgc ggccgggtgg gctggttgcg gtggcgagcg tcgactacgg
     1981 cggccttgtc ctggccggac cgcaggtcga cgtggttcgc cgcttctacg cggtccggga
     2041 acagctctgg ctcagcgagg gggccgaccc ctacctgggt cggcggctcc gtggcctcct
     2101 gctcggcgcg ggtttgcacg acgtcgtcgc cagctcgacg tacatctgtc acgggacggc
     2161 gcgagccgta cgccagttcg gccagggccg cgccgaggac tgccaggacg actggtacgt
     2221 cgactcggcc ctgcgacacg gcctcgcaac cggcaaggag atcgaggaga tgcggcgtgc
     2281 gtggcttgag tggtcgcagg ctccgacgtc gtacgccgca ttctgctggt gccgtgcact
     2341 cggctggaag ggctgagcaa cgctccgtcc gagccgaggc gcccgacgta cacgcggttc
     2401 atcctggccg gatcccgcgt agcctgcgaa gcacacacga ccagggagga caagccatgc
     2461 gcagcgagtt gttcgggaca gccctcgagt ccgcgacaac cgaccggttc acgttgcaga
     2521 acgcccgcct gcttcgagtg gcgcttgacg gggaagtgat ggcgcgacag ggcgcgatga
     2581 tcgcgtacca gggcgaggtc gacttcgcct atcagggttc cggcgggatg gggaagctct
     2641 tcaagaaggc cttcacgggt gagggcatgt cgctgatgaa ggtgagcggg aagggcgacg
     2701 tcttcttcgc cgacgacgcg atggagatcc atctcgtgaa cctggagaac gactccctaa
     2761 cagtcaacgg cagcaacgtc ctcgcgttcg actccacgct gacctgggac atcaagcggg
     2821 tggagggcgc gtcgatgatg gctggcggcg tcttcaacac caccttcacc ggcgtgggct
     2881 cgatcgcgat caccgcgcac ggaacaccgg tcgtgctcaa cgtcgacgct cctacgtacg
     2941 ccgacgtcca atcggcggtc gcatggtcga cgtcgctgag cacgacagtt cggcggaccg
     3001 ctggcgcggg tgcgctcatc ggccgcggct caggcgaggc ctttcaactg agtttctccg
     3061 gcgagggctt cgtcatcgtc caagcaagcg agggccggat cgtcgttccg cactctcact
     3121 gagccggctg atcggtacgg caagttcggg acactagacg tcgtccggac cggcgtctcg
     3181 tcgagccaat ccggccctga ccgagccgag gaacacatga acgaccagac cgatcaggat
     3241 gaggtcgacc accatctgca caaccgcgat gatccgggca gtctcggaga ccggggtgat
     3301 gtcaccgtag ccaactgtcg cgaaggtggt cacggtgaag tagagcgcgt cgatgcgcgt
     3361 catcggttct gagaagttgc tcgtctcgtc gtactggtcc atcaggaagt aggtggtgga
     3421 gaacaacagc acgacgagcg ggaacgaggt ggcgagcgac tcgattgccc gcacccgcgg
     3481 atacggcgat cgggcaatgg cgtgtgtctg ccaggcgagc aggcaggcga tggccacgag
     3541 cccgagcgac agcgcgaggg ccgtgctgaa tgcactgctc ttgtcgaacg gcaacgtgaa
     3601 gtacagcacg aagacggcga ccgtgacgat gagtgcccgc aacagcgggc gggccatcgc
     3661 tcggcgccga ccggcgcggg tgagactgtc gtaggacatt tcacgcatcg cgagccctac
     3721 acgttcaccg agcacccatg atcgggcagt gcgccttctc ccgaggtccg gtcgccgcct
     3781 cgtgcgttcg agggctgcag catgccatgg acggatcctt ttgcatggca ctgggtgtgt
     3841 cctcacccag cgcgggtgag cgggccgagg agttcctcgc cgcgtaagac catcaggtcg
     3901 ctggcggctc ggtggcggtt ggcgtgtcgt ccgaggaccg tgggcgaggc tcgagtgcct
     3961 tgctcaggac cagttcgcgt ccgtcctcgt cgtcccaacg ctcgcccacc tcgacgaagc
     4021 cgcggcgctt cagcaaccgc aacgagggca cgttgtccgg gcccacgctg gcgtcgacgc
     4081 gcgtcacgcc tcgggcggag agccaggtca ggaacaggtc gacgatctgc ccgccgacac
     4141 cctgcccgcg cgccgagggc acgacggcgt agccgatctc gaccgcgcca ccggcgtccg
     4201 gagctgcatg tgacccgatg cggccgacga atccgccgtc ctggacagcg gcatgcagca
     4261 ggaagggcgc agcctccgga tcggagtcca gcgcctccac ccggtagcgc agcacccgac
     4321 ggtcgtccgc cggccagacg acgccggaca agtctggttg gccggctgcc cagtcggact
     4381 cgccggcgta cacacgcctg atcgactcgg ccgacaacgc gatcagggag aggctcacag
     4441 tccggcggcc gcagcgagat cgccccgaat cgcgtccagc cggccggctg ccgagatgcg
     4501 agcggcttcc acgtcggagt cgtcgactgg cacgaccact tcgaggtagc acttgagctt
     4561 cggctcggtt cccgacggcc gtacgacgac ccgaccgccc tcggccaggc ggtagcgaat
     4621 cccctcggtg ggcggcagcg agtccgtacc ttcgttcagg tcgtcggtct gtacgacgtc
     4681 gtagccgccg agctgcgatg gcggcgtctc acggagacgt tccagcgcct ccccgatgcg
     4741 cgtcacgtcg tcgacccgga ccgacagctg gtcggtcgca tacagcccga accgcgcggc
     4801 gatctcgtcc aaccggtcga gcagcgactt gccggacgcc ttgagctcgg cagccagttc
     4861 ggccacccgg accaacgccg acacgccgtc cttgtcgcgc accaggtcag gagcggcgca
     4921 ataacccaac gcctcctcgt agccgaaggc caggcccggc acgcggccga tccatttgaa
     4981 gccggtcaac gtctcggcga agtccacgcc ggcggcccga gcctgttcgc cgagcaggga
     5041 cgacgacacg atcgtcgtcg caaagacacc ccggacaccg cggcgcagca ggtggtcggc
     5101 cagcagcgcg cccacctcgt cgccgcgcag catctgccac ccgtggtccg tcgacaccgc
     5161 cacagcgcat cggtccgcgt cggggtcgtt cgcaaccacg atgtcggcat cgttctcggc
     5221 ggccagcgcc atcgccaggt cgatcgcccc gggctcctcg ggattgggga aggacaccgt
     5281 cgggaagtcc gcgtccggaa cagcctgctc gggtacgacg atcggggcgg tgaaccctgc
     5341 ccgcaccaac accttctcga ctgtctcgtc acccacaccg tgcagcggcg tatagaccat
     5401 ggtcaggtcg cgggcagagt cggatgcggg cagggcggcg acggcggcaa tgtaggactc
     5461 gacgatgtcc tcgccgagca cggtccagcc tgcggagcgc ggcacctcgg cgaccgcctc
     5521 gaccgcgtcg atcttcgccg agatgtccgc gtcggcaggt gggacgatct ggctcccgtc
     5581 gccgagatag accttgtagc cgttgtcctg tggtgggttg tggctcgccg tgaccatgac
     5641 gcctgcgacg cagccgaggt gccggatcgc gaaggccaca agaggcgtgg gcagcggccg
     5701 gggcatgacc acagcgtcca ggcccgctcc gcacatcacc tccgcggtgt ccctggcgaa
     5761 gacctcggac ttgtgacgtg cgtcgtagcc gatgacgacg gtcccggacc ctgccgtctc
     5821 gcgcaggtac gctgccaagc cggcggccgc ggcggtcacc accacacggt tcatccggtt
     5881 cggcccggcc gccagcgcgc cacgcaggcc ggccgtcccg aactcgagcc ttccgtcgaa
     5941 ccggtcgatg aggtccgcca tggccgccgg gtggccgccg tccgccagcg acagcaggtc
     6001 ggccagctcg gcgcgcgtct cctcgtccgg gtcgtcggcg atccacgccc tcgtccgtcg
     6061 acgcagctcg atgggaacgt tcaccgtcgt ctccttgcgc gtgccctgat catgtccctg
     6121 atcatgtccc tgatcatgtc gctgtcacgt cgaacggccg ccggtcgaac tcagcagtgg
     6181 tgaactcgcc ccccaccctc gtgatcgcga tggctggcgc acgagacgtc accaactcgg
     6241 gagcgacgat cgccgcgtcg tctcgtggtt cgaagccgag cgccctaccc ggtcccagat
     6301 cccaccaggc gtcgcggttg gccgagatcc cgtacaccac gcggaagcct tcgaagtcgg
     6361 cggtcagcgc tgcgtcgacc atggccgtgc agtcgtcgta cgacaaccac gtcgacagat
     6421 gtcgtgacga gtcagggcgc tgccgaaagc tgccgatccg catcgccaca ctggagacgc
     6481 cgtaccggtc ggcatacagg ctcagcaacg cctcccccgc gaccttgccg acgccgtaga
     6541 acgtgtcggg gcgcgcgggg gtgtcgactc cgatcggccc ggagtccggc cgcggagtca
     6601 tgccgaccgc gtggttgctg ctggcgaaga ccatccggcg tacgccgtgc tggaccatcg
     6661 cgtcgaggat tgcggccgtg ccgaccacgt gaccgtgcag gatgtccggg agggcagcct
     6721 cgctcgcgac accggcgagg tggacgaccg cgtccatacc gtcgaccgcg gcagccgcga
     6781 cggccgggtc ggcgcagtcg ccgaccacga actcgcccgg atacgcagca ccgcagtcct
     6841 ccggggcgtg ccatgcggcc aggtcgaggc cgcgcatctg gtgccgcgcc gagaggcgat
     6901 ccgacaggac agaaccgatg tggccggcag cccccgtgac gagaacgcgc atcagactcg
     6961 cggcacgatc gcagcgagca ggtcacccat gcgggtggcg gcttgccgac ccgcctcgag
     7021 gacctcgtcg tggttgagcg gcgcacccgt caacccggcg gcagcgttgg tgacgagcga
     7081 gatgccgagc acctccatgc ctgcctcgcg cgcggcgatg gcttcgagcg tcgtcgacat
     7141 gccgaccagg tcgcctccga tggtgcggac catcgcgatc tctgcaggcg tctcgtagtg
     7201 gggcccgggt agctggacgt agacgccttc gtcgagcgtc gggtccgcgt cacggcacag
     7261 cgcacgcaac cgcgaggagt acaggtcggt gaggtcgatg aaccgcgcgc cgacgatggg
     7321 ggagctgccg gtcaagttga tgtggtcgcg gatcagtacg ggtgtgcccg gcgaccattc
     7381 cggtcggacg cctccgcaac cgttggtgag gacgacgacg cgacagccgg ccgctgccgc
     7441 catccgtacc ccgtgcacga cagcggcgac gccgagcccc tcgtagaagt gcgtgcggcc
     7501 gaggaacacc agggcacgaa cgccggatgc gtcgacggag cggatcttgc cggcgtggcc
     7561 ctcgacggca ggaggagcga agccgggcag atcggtcgtg gagatctcgg caccggtggc
     7621 accgagcgcg tcggcggcgg gcaaccaacc cgaccccatc acgagcgcca cgtcgtgacg
     7681 ggccagacct gtcagctcac cgaggcgctt ggcggcctgg tcggcgacgt cgtacggcga
     7741 cggcgtggtc acgtccggca ccggatcgct catgtggaga ccgtaccgcg cggctcattc
     7801 cgatacggat ctgcagggac gggtgcggag gtcgtgcacg tagtcgtcgg gagctccggc
     7861 ggcctgggca gcttcgctga gcaccccgag gtagctcgcc gacgggaggc cgccttcgta
     7921 cgcatcgagc acatagagcc acgcgagctg ttcgccgtcg agcgtcgaca cccgcacccg
     7981 gatcttgccg tagacggagc tctcccattc gtcgaggtgt ctctcgtcct cctgggccag
     8041 ttcgtacagc gccacgaaca cctgctcacc cggcgcttcg acgacggtcg ccagggcgcc
     8101 gtcccagccg tgctcctcgc caccgaaggt gagtcgccac ccggaaagcc agccggaacc
     8161 tcgcgtcgga gaacgcgggc agcgttcggc catccgtgcg gggtccatgt tcgagccgta
     8221 cgcggcatag agcaccacgg cccgcaggtt atcgcgagcc cggttatcgt gactgcgtga
     8281 cgcgcgtcgt gatcatcggt gggggaccgg gcggctacga ggccgcgttg gtcggcgccc
     8341 agctcggtgc ggaggtcacg atcgtcgacc gggacgggat cggcggatcc gccgtcctga
     8401 ccgactgcgt tccctccaag acactcgtcg ccacgtctga gctgatgaca tcggtccagg
     8461 agtccggcga actcggcgta cgtcttgctg gtcagccgcc ggacgccggg cacagtgcgt
     8521 cggtggacct cgcggtggtc aacgaacggg tcaggaagct ggcggctcag cagagcgccg
     8581 acatcacgcg ccggctcgat caggaggacg tgcgcatcat ccgcgggacc gggcggctcg
     8641 acggcccgga cacggtcgtc gcggccacgg cggacagcga ggtgcggctc gaggccgacg
     8701 ctgtgctcgt cgccaccggc gcgcacccgc gggtgctgcc gagcgccgaa ccggacggcg
     8761 agcgcatcct cacctgggag caggtgtacg acgtcgacga gttgccggcg cggctggtcg
     8821 tgatcgggtc gggagtgacc ggggccgagt tcgccagcgc gtacaacgcg ctcggtgtcg
     8881 acgtcgtgct ggtcagcagc cgtgaccggg tcctgccagg tgaggacgcg caggcggccg
     8941 aagtcctcga ggacgtgctg aagcgccgcg gcatgaccgt cctcggtcgt agccgggccg
     9001 cggacgtcgt acgcgagggc gactccgtcg ccgtgaccct caccgacggc tcctccgtgg
     9061 agggatcgca ctgcctgctc gcggtgggct cgatccccaa cacggccggc ctgggtctgg
     9121 aggaggcggg ggtcacgctg ggcgacggtg gcttcgtgac cgtcgaccgg gtctcccgga
     9181 cgacagggcg tggggtatac gccgctgggg actgcacggg cgtgttgatg ttggcatccg
     9241 tggcggccat gcaggggcgg atcgccatgt ggcacgcgct cggggacgcc gtctcaccgc
     9301 tcgacctcaa cacggtctcg tcgacggtgt tcacgtcccc ggagattgcc accgtcggct
     9361 acagccagca ggcggtcgat gcgggagtga tcgaggcgaa ccagcagatg ctcccactgg
     9421 ccggaaatgc ccgggccaag atgcagggcg tgcacgacgg cttcgtcaag ctcttctgcc
     9481 ggcccggcac cggcatcgtg gtcggtggcg tcgtcgtggc gccgcgggcg agcgagctca
     9541 tccacgcggt ttcgctgtcg gtcgaagcca gcctgacggt cgacgaactg gcgcacgcgt
     9601 tcaccgtcta tccgtcgatg agcggttcga tcgccgaagc cgcccgccgc ctccacaccc
     9661 gctgaccgcc tcccaccgca cattcccgcc cccgtgcttc ccgcgaattg cgggaaaggt
     9721 caggccgaaa cgcccttgtc agggtccact ttcccgcatt ccgcggccgc ccgtaggcgg
     9781 cgttcacctc gtccgcggag cggcttggcg aagcggtgga ggcgtcgtag cagtcttcgg
     9841 tagccgagct ggctgagact ggatcgttcg acctcagtca accaaggctc gagacgtgtc
     9901 cggtcgtgcc ggaggccgag ggcgttctcg gcatcacgaa ggatctgtgt cggattgtcg
     9961 aggatctcga cggaggtgta tccatagcgc tcccagttcg acctggcgag caacttgtca
    10021 cgggcgaggt cgcgacgatg gcgctcgcga tcacgatgat tggctccgtc gtactccggt
    10081 agccgccttg tctccttgat gcgcagatcc gcacgggcga cgacctctcc gtcggcgtcc
    10141 cggatggggt attgcgcttc cacggggatc ttgcacagga cgtgaaagag ccggagcacg
    10201 ctctcccatc gtgactcgct gcgaccgtcg accagcgcga gcgcccgacg cagcatcggc
    10261 aacccgcgtt gccgcgagcg gattgcctgc caggtgtcgt cctcggtaca cagctgctgt
    10321 tgcaggaacc cgtcgattgc gatgaccaga tcgatcagtt gcaggtcctc agcgagctga
    10381 ccgatcacca gggccggatc gagaacgggg acgccactga caacgatcgg tggtgggagt
    10441 gctgcccggc tacgggcgac gtacagtccg gcgcgttcgg gtcgctgtgc ccccggcggg
    10501 atcgtcacct ggagaggaag ccagtcgggg agagccggca gccaggtcga ccagagccga
    10561 gctgcggtca ggtggctgca cgcagcgtcc cacggaagaa tcggtcgaac gtgtcggcag
    10621 cggtcgatga ggtctgcgtc cgcctcgcgc ctgacgtaga ggccgtggct gatcggggtc
    10681 catcctggtc cgcgcatctg cgaccgtgtc cgtccggcct ggagcgcatc gcgaagcaac
    10741 acagattcgt ccatccgaca agatcaccgc ggatgggcat cgcgctgcgg tcgacgcagc
    10801 atctttgtgg accaactccc ggccctcccc aaccctgtgg gctcccgcga attgcgggaa
    10861 aagtcaggcc gaaacgccgc tgtgaggggc cactttcccg caattcgcgg gaaccaggca
    10921 g
//
LOCUS       DATBFZ010000043         2529 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_357876, whole genome shotgun sequence.
ACCESSION   DATBFZ010000043 DATBFZ010000000
VERSION     DATBFZ010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2529
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357876"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..970
                     /locus_tag="VH419_01335"
     CDS             <1..970
                     /locus_tag="VH419_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042289.1"
                     /translation="QPDKTAPTASYTVSQGGPNDSHATITLDPGSLKDNVSSSVDITQ
                     DVDWGDGAHASGNGKTTTSFSHDYLQTKQIYHPVVTLTDKAGNSRPYTFTVVVADTTH
                     PTGTFSVSPSAAWAKWTSVSVKPVSLGDDLSQPGNIGWSVNWGDGTSQSSTTGSASSL
                     SHRYATAGSFTPAVTITDEAGNTWNPAPASTVAVKVDSKAPRVRVIAPKVHKQFVRSW
                     RTLNGRATDAGTGVRKVRVAAIEKRGKVWYGYRPATKTWVRGGKTRAAAWRASRVAPV
                     TATVAHTWSLPLRHLTKGVLVSKVSGVDNVGNKGAWKVYQRSLTRR"
     gene            1123..>2529
                     /locus_tag="VH419_01340"
     CDS             1123..>2529
                     /locus_tag="VH419_01340"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY7042290.1"
                     /translation="MLLLERDGHVASLVQYAEEARAGHGRTVLMAGEAGVGKSSLLDA
                     LEQELTDALWYWGTCDGLFTPRPLAPLLDIADQIGGELKLLCDSGAPRDQLFSELVRH
                     LHTPDRLTVLAIEDVHWADEATLDLLRFLGRRLRDSATLLVITYRDDGLAQDDPLRVA
                     VGELSMHRTTRRLSLAPLSTDAVAGMAAAAGADPARLYALTGGNPYYVCEVLQTAEAA
                     LPESVRDAVLARVAAVSANAREVLDVAALMGARVDHQLLERATGASSAILDELLACGV
                     IVSDADQLRFRHEIARMAIQSAIPPHRIRSTHRTILDALLQAGLQDDARLAFHAEGAG
                     DVGLVLEYAPRAGRRASELAAHREAAAQFERSLRAADQADDRTTASLYDDLAGELALV
                     DRWQDSANAREEALRRWRAVGDPLREGDSLRRYSRVMWRLCRGDEADSAAVKALELLA
                     PLGPSPELAWAYANLANNK"
BASE COUNT          416 a          881 c          840 g          392 t
ORIGIN      
        1 tcagccggac aagaccgcac ccacggcctc gtacacggtg tctcaaggtg ggcccaacga
       61 cagtcacgcg acgatcacgc tcgaccctgg ttcgttgaag gacaacgtct cgtcgtccgt
      121 cgacatcacc caggacgtgg actggggaga cggagcgcac gcctcgggga acggcaagac
      181 caccacctcg ttctcgcacg actacctgca gaccaagcag atctaccacc cggtcgtcac
      241 gctgacggac aaggcgggaa actctcgccc ctacacattc acggtcgtcg tcgctgatac
      301 gacgcacccc accggcacgt tctcggtgag cccgtcggcc gcctgggcga agtggacgag
      361 cgtctcggtg aagcccgtga gccttggcga cgacctgagc caacccggca acatcggctg
      421 gtcggtcaac tggggcgacg gtacgtcgca gtcctcgacc accggatctg cgtcctcgct
      481 gtcccaccgc tacgcgacgg ccggcagctt cacaccagcc gtcacgatca ccgatgaggc
      541 aggcaacacg tggaaccccg ccccggcttc caccgtggcc gtgaaggttg actcgaaggc
      601 gccgcgggtg cgggtgatcg ctcccaaggt ccacaagcag ttcgtgcgaa gctggcggac
      661 gctgaacggc cgcgccaccg acgccggcac cggtgtgcgc aaggtccgcg tggccgccat
      721 cgagaagcgc ggcaaggtct ggtacggata ccgcccggcc accaagacat gggtccgcgg
      781 cggcaagacc agggcagccg cgtggcgcgc ctcccgggtc gcccccgtca cggccaccgt
      841 ggcgcacacc tggtcgctgc cgctgcgtca cctgacgaag ggtgtgctgg tctccaaggt
      901 ctccggagtg gacaacgtcg gcaacaaggg tgcctggaag gtctaccagc gctcactgac
      961 ccgccgctga ccagagctgg ccagagttgg cggttttcgg gcgtggcgtc acggctcata
     1021 tccaccggaa tcgggtcgat gggtaccggt caccgtgccg agccggagcg gattcggtaa
     1081 tgcgggtgta acgctcgccc ggaagggcgg acaatcccaa gcatgctgct gctcgagagg
     1141 gatggtcatg ttgcctccct cgtccagtat gccgaggagg cgcgagccgg ccacggccga
     1201 accgtcctga tggcgggtga ggcgggcgtc ggcaagtcgt cattgctgga cgcgctcgag
     1261 caggagctca ccgacgcgct ctggtactgg ggaacctgtg acggcctgtt cacgccacgt
     1321 ccgctggcgc cgctgctgga catcgccgac cagatcggcg gcgagctgaa gctgttgtgc
     1381 gactccgggg cgccacgcga ccagctcttc agcgagctcg tccggcacct gcacacgccc
     1441 gaccggttga ccgtgctcgc catcgaggac gtccactggg ccgacgaggc cactctcgac
     1501 ctgctgcgtt tcctcggccg ccggctccgc gacagcgcga cactgctggt gatcacgtat
     1561 cgcgatgacg gcctcgccca ggacgatccg ttgcgtgtcg cggtcggcga gctctcgatg
     1621 catcgcacga cccgtcgcct cagcctggcg ccactgtcca ccgacgccgt tgccggcatg
     1681 gcagcagcgg cgggtgccga tccggcccgg ctgtatgcac tcaccggcgg gaacccgtac
     1741 tacgtctgcg aggtgctgca gaccgccgag gcggcacttc cggagtcggt gcgcgacgcc
     1801 gtattggctc gcgttgccgc tgtctctgcg aacgcgcgtg aggtcctcga tgtggccgcc
     1861 ttgatgggtg ctcgcgtcga ccaccagctg ttggagcggg ccaccggggc gagctcggcc
     1921 atcctcgacg agctgctcgc ctgtggcgtc atcgtcagcg acgccgacca gctccggttc
     1981 aggcacgaga tcgcgcgaat ggcgatccag tcggcgattc ccccacatcg gatccgttcg
     2041 acccatcgga caatcttgga cgccttgctc caggccggtc tccaagatga tgcacgcctt
     2101 gcctttcacg ccgaaggagc cggcgacgtc ggcctcgtcc tggagtacgc gccccgcgca
     2161 ggccgacgtg catccgagct cgccgctcac cgtgaagcgg cggcgcagtt cgaacgctcc
     2221 ctgcgggctg cagaccaagc cgacgatcgg acgacggcgt cgttgtacga cgacctggca
     2281 ggcgagctgg cactcgtcga ccgttggcag gattcggcga acgcacgcga ggaggcgctg
     2341 cgacgttggc gtgcggtcgg cgatcccctt cgcgaaggcg acagcctgcg acggtactcg
     2401 cgggtgatgt ggcggctctg caggggcgac gaggccgaca gcgccgccgt gaaggccctc
     2461 gagctgctcg cgccgctcgg acccagtcct gaacttgcct gggcctacgc caacctcgcc
     2521 aacaacaag
//
LOCUS       DATBFZ010000044         4069 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_5267084, whole genome shotgun sequence.
ACCESSION   DATBFZ010000044 DATBFZ010000000
VERSION     DATBFZ010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 4069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4069
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5267084"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1609
                     /locus_tag="VH419_01345"
     CDS             <1..1609
                     /locus_tag="VH419_01345"
                     /inference="COORDINATES: protein motif:HMM:NF016071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HEY7042291.1"
                     /translation="GEFGSNDGQDNAERITQFGLGAGKKPAVRVLGQLSPPALFTEPL
                     ETEEEQGSIPLATETGIPDETSGVEVDSEIGDGEFGSAGTGSGDFDFFKVTASAGEAI
                     RANTAGSDFDTILIVYDADGNILASNDDANGTFQSDLTFLAPADGDYFVLVTGFLCLP
                     EDPFDPSSGTGAESEGAYHLEITVGPLDQDFYAVRLKPGDVLGGMIKGAAIDMTVHKP
                     NGEERIVSEFADASFIYPMQSPLPGAGGDGNASFAYVAEEGGWYTVSTSNGDGPYQMQ
                     LEVYRPGSEAAGPGKTQKIFLDFDGERVNTGVFGGFGVVTLSPLSAFLGRWGLPNGRL
                     NAVIDQVVATVKENIKQDLVAKGLNPNVKVKVLNSRDDPDPFGNPNVSRVIVGGTIAQ
                     SGVPTIGIAQSIDPGNFAHEETALVLLDTVSDPDPNDDASFNFYFGPGTNKVKFIGTA
                     LGNVVSHETGHFVGSFHVDQFNDVLNLMDQGGNFALLYGVGDDGVGGTADDPDVDFGV
                     DTYNPNEGFAGLENTLNNTAWAFRPGQ"
     gene            complement(1684..2109)
                     /locus_tag="VH419_01350"
     CDS             complement(1684..2109)
                     /locus_tag="VH419_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY7042292.1"
                     /translation="MINHVSLQSVWVHDIDEARDFYVDKLGFVLNTDVTMGGYRWCTV
                     NHPNQPDLQLVLSLPGPPLDEDDAEQVRRMIGKGAMSGGGLDTDDCRKTYEELKDKGV
                     EFLQEPQDRPYGVEAVLRDNSGNWWVLVERKGFDPADLQ"
     gene            complement(2153..2584)
                     /locus_tag="VH419_01355"
     CDS             complement(2153..2584)
                     /locus_tag="VH419_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012921974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEY7042293.1"
                     /translation="MSSVAVPTALLPHLRRARDLADRSYAQPLDVGRLAAQASTSKYH
                     FIRSFTSVYGETPMRCLSRRRIERAQDLLRATNLTVTEVCHLVGYTSLGTFSTRFRDL
                     VGMSPSEYQASQPAARIPGCYIFMLGLQPLGSRNLEEAAPN"
     gene            complement(2654..2926)
                     /locus_tag="VH419_01360"
     CDS             complement(2654..2926)
                     /locus_tag="VH419_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017935783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HEY7042294.1"
                     /translation="MPRGKTPGPSVKDDKTYEAVRRQGASKEKAARIANASANQGRKT
                     VGRRGGKAGTYEDMTKQELYDRAKRIGIEGRSTMSKRQLISALRHH"
     gene            complement(3080..3850)
                     /gene="lexA"
                     /locus_tag="VH419_01365"
     CDS             complement(3080..3850)
                     /gene="lexA"
                     /locus_tag="VH419_01365"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012922273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEY7042295.1"
                     /translation="MAKTSKTAKSPRSPAPATGQEDTHAVHELPDGPPDSTGLTPRQR
                     RVLAVIRDSVVARGYPPSMREIGQSVGLTSSSSVAHQLRVLESKGYLRRDPNRPRAVE
                     VLLPDNQPSRAVGPAAETAYDETDIGDSRPAATYVPVVGRIAAGGPILAEERVEEIFP
                     LPKRLVGEGTLFLLEVKGDSMIDAAICDGDFVVIRQQPTAENGEFVAAMIDGEATVKT
                     LQKKAGSVWLLPHNAAYEPIDGTHATILGKVVTVMRKV"
BASE COUNT          654 a         1293 c         1389 g          733 t
ORIGIN      
        1 tggcgagttc gggtccaacg acggccagga caacgccgag cggatcaccc agttcggtct
       61 gggcgccggg aagaagccgg ccgttcgagt gctgggccag ctgtcaccac ccgccctgtt
      121 caccgagcct ctcgagaccg aggaggagca gggctcgatc ccgctcgcga ccgagaccgg
      181 tatcccggac gagaccagcg gtgtggaggt cgacagcgag atcggcgacg gcgagttcgg
      241 ctcggcgggc accggatcgg gggacttcga cttcttcaag gtcaccgcgt ctgccggcga
      301 agccatcagg gccaacacgg cggggagtga cttcgacacg atcctgatcg tgtatgacgc
      361 cgacggcaac atcctggcca gcaacgacga cgcgaacggg acgttccaga gcgacctgac
      421 gttcctggct ccggccgacg gtgactactt cgtgctggtc accggcttcc tgtgtctgcc
      481 ggaggatccc ttcgacccca gcagcggaac cggcgccgag tccgaggggg cttaccactt
      541 ggagatcacc gtaggtccgc tggaccagga cttctacgcg gtccggctca agccgggcga
      601 tgtgctggga ggaatgatca agggcgcggc gatcgacatg accgtccaca agcccaacgg
      661 cgaagagcgg atcgtgtcgg agttcgccga tgcgtcgttc atctacccca tgcagtcgcc
      721 gctgccgggg gccggtggtg atggcaacgc gtcattcgcc tacgtcgccg aggagggcgg
      781 gtggtacacc gtttcgacgt ccaacggtga cgggccgtac cagatgcagc tcgaggtgta
      841 ccgtcccggc tcggaggctg ccggaccggg gaagacccag aagatcttcc tcgacttcga
      901 cggtgagcgg gtcaacaccg gcgtgttcgg cgggttcggc gtcgtgacac tgtcgccact
      961 gtcggcgttc ctcggccgat gggggctgcc caacggccgg ctgaacgccg tgatcgacca
     1021 ggtggtcgcg accgtgaagg agaacatcaa gcaagacttg gtggccaagg ggctcaaccc
     1081 gaacgtcaag gtgaaggtcc tgaacagccg cgacgaccct gacccgttcg gcaacccgaa
     1141 cgtttcacgg gtgatcgtcg gtggcaccat cgcgcagtcg ggcgttccga cgatcgggat
     1201 cgcgcagtcg atcgatccgg ggaacttcgc gcacgaggag acggcactgg tgctgctcga
     1261 cacggtcagt gacccggacc cgaacgacga cgcttcgttc aacttctact tcgggcccgg
     1321 caccaacaag gtgaagttca tcggcaccgc actcggcaac gtcgtgtcgc acgagaccgg
     1381 tcacttcgtg ggcagcttcc acgtcgacca gttcaacgac gtcttgaacc tgatggatca
     1441 gggcggcaac ttcgcgctgc tgtacggcgt cggtgacgac ggcgtcggcg gcacggccga
     1501 cgacccggat gtcgacttcg gtgtcgacac ctacaacccg aacgaagggt tcgccggtct
     1561 ggagaacacc ctcaacaaca ccgcctgggc gttccgtccg ggtcagtaac cgaagcccac
     1621 gattcgtccg accggccgca gggtgacacc tgcgcgccgg ccggacgaat ccggggttca
     1681 gtcctactgg aggtctgcag ggtcgaaccc cttgcgttcg acgagcaccc accagttgcc
     1741 ggagttgtcg cgcagcaccg cctcgacgcc gtacgggcgg tcctgcggct cctgcaggaa
     1801 ctccacgccc ttgtccttga gctcctcgta cgtcttgcgg cagtcgtcgg tgtccagccc
     1861 gccgccgctc atcgcgccct tgccgatcat ccggcgtacc tgctcggcgt cgtcctcgtc
     1921 caacggcggg ccgggcaagg acagcacgag ctggaggtcc ggctggttcg ggtggttgac
     1981 cgtgcaccag cggtagccgc ccatggtgac gtcggtgttc agcacgaagc cgagcttgtc
     2041 gacgtagaag tcgcgtgcct cgtcgatgtc gtggacccag acagactgga gcgagacgtg
     2101 gttgatcatc gtgatacccc tctggtcttt gttccccacc acgctagagc gctcagttcg
     2161 gtgctgcttc ttccagattg cggctcccga gcggttgcag gccgagcatg aagatgtagc
     2221 agccggggat ccgggcggcc ggctggcttg cctggtactc cgacgggctc atgccgacga
     2281 ggtcgcggaa gcgtgtggag aacgtgccga gactcgtgta gccgaccagg tgacagactt
     2341 cggtgacggt caggttggtg gctcggagga ggtcctgggc gcgttcgatc cggcgccgcg
     2401 acaggcagcg catgggcgtc tcgccgtaga ccgaggtgaa gctgcggatg aagtggtact
     2461 tcgaggtcga tgcctgcgca gcgaggcgtc cgacgtcgag tggttgcgcg tacgaccggt
     2521 ccgcgagatc acgggcgcgc cgcaggtgcg gcagcagcgc ggtcggcact gccacggaag
     2581 acacgtcgtc aaccctagag cccgtcccca tgtcctggtg gcgagaccgc gggcatggcg
     2641 atgtgctgct gcttcagtgg tggcgcagcg cgctgatgag ctgtcgcttg gacatcgtcg
     2701 aacgtccttc gatgccgatc cgcttggctc ggtcgtagag ctcctgcttg gtcatgtcct
     2761 cataggtccc ggccttaccg ccacgtcggc cgacggtctt gcggccctgg ttggctgacg
     2821 cgttggcgat cctggccgcc ttctccttgc tcgctccctg tcggcgtacg gcctcatagg
     2881 tcttgtcgtc cttgaccgac ggtcccggtg tcttccctct gggcatggcg aacctcctga
     2941 tgacggatcc tctgctgacc tggtgcccgg aggtcctttg cccaaacgtg agctgagtcg
     3001 gcgcaagcgg aaacaaaccg ccaaccggcg agaggattac ggtcgaacgt cgacccttgg
     3061 ttgagaccag ctgcgacggt tacaccttcc gcatgacggt gacgaccttg ccgaggatcg
     3121 tggcgtgggt gccgtcgatc ggctcgtacg cggcgttgtg ggggagcagc cagaccgagc
     3181 cggccttctt ctgcaaggtc ttgacggtgg cttcaccgtc gatcatcgcg gccacgaact
     3241 cgccgttctc ggcggtgggc tgctggcgga tcacgacgaa gtcgccgtcg cagatggcgg
     3301 cgtcgatcat cgagtcgccc ttcacctcga gcaggaacag cgtcccctcg ccgacgagcc
     3361 gcttcggcag cgggaagatc tcctcgacgc gctcctcggc aaggatgggg ccgccggcgg
     3421 cgatccggcc gacgaccggg acgtaggtgg ccgctgggcg ggagtcgccg atgtcggtct
     3481 cgtcgtacgc cgtctcggcc gccggaccca ccgcgcggga cggttggttg tcgggcagca
     3541 ggacctcgac ggcacgtggt cggttggggt ctcgacgcag atagcccttg ctctcgagca
     3601 cacgcagctg gtgtgccacc gacgatgagc tggtcagtcc gacggactgg ccgatctccc
     3661 gcatgctcgg cgggtagcca cgggccacaa cagagtcgcg gatcaccgcg aggacccgtc
     3721 gctggcgtgg ggtgagcccg gtggagtcgg gtggaccgtc ggggagctcg tgcaccgcgt
     3781 gggtgtcctc ctgcccggtc gcgggggcgg gcgatctcgg cgacttcgcg gtcttggacg
     3841 tcttggccat ctcgtcacgc cttcgtttcg gtctggtcgt ctcgcagacg acggtagacc
     3901 tgacggccga cgctttcaaa cacctgttcg aatcggcgtg tcctggcggt gtcgagagga
     3961 caggttcccc ggttggggtg tcgctcgaac acgcgttcga tcaatgcgca ccattgtcgg
     4021 tggcccgcgc tatcgtttca aacatgtgtt cgatcgaacg tgtcttcga
//
LOCUS       DATBFZ010000045         4064 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_4939444, whole genome shotgun sequence.
ACCESSION   DATBFZ010000045 DATBFZ010000000
VERSION     DATBFZ010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 4064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4064
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4939444"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(454..2211)
                     /locus_tag="VH419_01370"
     CDS             complement(454..2211)
                     /locus_tag="VH419_01370"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012920238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HEY7042296.1"
                     /translation="MPQLRVALAQVNPTVGDLTGNADLIASAVAEAAEAGAHLVVTPE
                     MCLTGYPIEDLAFRPSFVEASRRAVVDLARRLQSAGHGDVAVVVGFLDRAEPPQQGVG
                     IPKGSPQNAVAVLYAGEVRARQAKHHLWNYGVGDEIRNFVPGTTLSVIQVRGVDVAIA
                     ICEDIWQDGPSAAADAAEAALLVVPNGSPYERHKDDARLELCARRAREGDCALAYVNL
                     VGGQDELVFDGDSLVVDTSGELVARAFQFVPQVLVVDLDLPSATDSMPPPGTEFFGLR
                     VERTILSTEPVPSYDAERAALAPRLDDLAEVYHALVLGLRDYVRKNGFRTVLLGLSGG
                     IDSTLVGAIACDALGPGNVFGVSNPSEWSSEHSRTDAAELARRTGLQLDTVAIAPMVD
                     AFRAAVKVDGLAEENLQARIRAVVWMALSNQHGHLVLACGNKSELSVGYSTIYGDAVG
                     GFAPIMDVPKTMVWELARWRNADAERRGEQPPIPEHAIEKPPSAELRPGQLDTDSLPD
                     YHLLDDILDDYVEADRSAPELVAQGFDAAMVERVAWMVDRAEYKRRQYPPGPKISARN
                     FGRDRRLPITNAWREQLRR"
     gene            complement(2273..3763)
                     /locus_tag="VH419_01375"
     CDS             complement(2273..3763)
                     /locus_tag="VH419_01375"
                     /inference="COORDINATES: protein motif:HMM:NF015083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme peroxidase family protein"
                     /protein_id="HEY7042297.1"
                     /translation="MAHGDADAGTQTPRSRFYDRGKFGRLFPSLPPFSADTPLIRDAL
                     AELGAPAGPMDAQDDLSDPITLITDPAKSQHNPNNPTLSAGMTFLGQFLDHDMTFDPT
                     SSLARAQDPESIRNFRIPALDLDSVYGGGPGVSPHLYDQTVDGGRTTFLTELIPGSAT
                     VSIDGCDRRDLPRNAQNVALVADPRNDENLMVSGLQLAMLRFHNRVLADVKEDLGAAF
                     TSDEIFAETQRVVRWHYQWMVLHEFLPKTVGAEIADDVLTNGPLFYTWRHDPFIPVEF
                     SVAAYRFGHSQVRPSYRANFGTSKTDVSQQFFGLVFDPTSTDAADPDDLRGGHRAPRR
                     FIDWQTFFDFGDGLVRPNKKIDTTLSSVLFALLGQSPGTPTSLATRNLLRNLTMRIPS
                     GQRVANAMQQPVLAATELDDLKALHLHNRTPLWFYVLREAEVVADGEHLGPVGGRIVA
                     EVLHGLVQGDRASYLRQDPEWTPTYGTDDSFGIVDLLEAAGAVVPL"
BASE COUNT          686 a         1354 c         1394 g          630 t
ORIGIN      
        1 acatccgttg gtcgccggcg ccggtttgca tggctaacca gccaaatcca tccctcccga
       61 ggcagacctg cctggctacc cgtccgtctg gatggctacc gaggcaaacc accaccgaca
      121 cacgaggtat gaggtttgcg atgtttacag cccaaaaggc gtgtaaacat cgcaaacctc
      181 cgatggggac agtgggtttg ccgtctgaac atcgcaaacc tcggtcccgt ccggccgcag
      241 atgtccgaaa ccgccgaccg ggcaccgacc ataaaggggc cgaaagccgg ccggccagcc
      301 cagcaggcgg caaacacctc ccgctcatat ctgccggctc accccagccg gcgccaacgg
      361 gagcgctgcg accaccccac ccatcgcgac ccgcccacgg aaaccggagg agcaccaaac
      421 ctgcccagcg acgggtgggg ttgctcggtt gcgctagcgc cgcagctgtt cgcgccaggc
      481 gttggtgatc ggcagccgtc ggtcacggcc gaagttgcgc gccgagatct tgggacccgg
      541 tggatactgg cgacgcttgt actccgctcg gtcgaccatc caggcaaccc gctcgaccat
      601 cgcggcgtcg aagccctgcg cgaccagctc cggcgcgctg cgatcggcct cgacatagtc
      661 gtccaggatg tcgtcgagca ggtggtagtc gggaagagag tcggtgtcga gctgaccagg
      721 tcgcagctct gccgacggtg gcttctcgat cgcgtgctct gggatgggcg gctgctcacc
      781 gcgccgctcc gcgtcggcgt tccgccaccg ggccagctcc cacaccatgg tcttggggac
      841 gtccatgatc ggggcgaagc caccgaccgc gtcaccgtag atcgtcgagt acccgacgga
      901 gagctcgctc ttgttgccgc aggccagcac caggtgtccg tgctggttcg acagcgccat
      961 ccagacgaca gcgcggatgc gcgcctgtag gttctcctcg gcgagcccgt cgaccttcac
     1021 cgccgcgcgg aacgcgtcga ccatgggagc gatggccacc gtgtcgagct gaaggccggt
     1081 ccgccgcgcc agctcggctg cgtcggttcg cgagtgctcg ctcgaccact cgctcgggtt
     1141 ggaaacaccg aagacgttgc ccggtccgag cgcgtcacac gcgatggcgc cgaccagcgt
     1201 cgagtcgatg ccgcccgaca gcccgaggag aaccgtgcgg aagccgttct tgcgcacgta
     1261 gtcccgcagc ccgaggacga gcgcgtggta gacctcggcc aggtcgtcca gccgtggtgc
     1321 gagggcggcg cgttcggcgt cgtacgacgg caccggctcg gtcgagagga tcgtgcgctc
     1381 gacgcgcagg ccgaagaact ccgtccccgg cggcggcatc gagtccgtcg cggacggcag
     1441 gtccaggtcg acgacgagca cctgcggcac gaactggaag gcccgcgcaa ccagctcgcc
     1501 ggaggtgtcc acgacgagcg agtcgccgtc gaacacgagc tcgtcctgac cgccgacgag
     1561 gttgacatag gcgagcgcac agtcgccctc tcgtgcccgg cgtgcacaga gctcgagccg
     1621 ggcgtcgtcc ttgtgccgct cgtacggtga tccgttcggt acgacgagca gcgcggcctc
     1681 ggccgcatca gcggcggcgg acgggccgtc ctgccagatg tcctcgcaga tcgcgatcgc
     1741 cacgtcgact ccacgcacct ggatgacgga cagcgtggtg ccggggacga agttgcggat
     1801 ctcgtcgccg actccgtagt tccacaagtg gtgcttggcc tgacgagcgc ggacctctcc
     1861 ggcgtacagg accgcgacgg cgttctgcgg cgagcccttc gggatgccga cgccttgttg
     1921 cggcggctcg gcacgatcga ggaagcccac gacgacggcg acgtcgccat gaccggcgga
     1981 ctgcaggcgt cgagccaggt cgacgaccgc tcggcgggag gcttcgacga aggacggccg
     2041 gaacgcgagg tcctcgatcg ggtatcccgt caggcacatc tccggtgtca ccacgaggtg
     2101 agcaccagcc tcggcggcct ccgccacggc cgaggcgatc aggtcggcgt tgccggtgag
     2161 gtcaccgacc gtcgggttga cctgggcgag ggcgacgcgc agctgcggca cgctgccagc
     2221 ctatcgaggg gccgaccgcg gtcctgtcgt ctccggcggg cgtccggtcc agctacagcg
     2281 gcaccacggc gcccgccgct tccagcaggt caacgatgcc gaacgagtcg tccgtcccgt
     2341 acgtcggcgt ccactccggg tcctggcgga ggtacgacgc ccggtcacct tgcaccaagc
     2401 cgtggagcac ctccgcgacg atccggccgc cgaccggccc gaggtgctcc ccgtccgcga
     2461 cgacctccgc ctcacggagg acgtagaacc acaacggtgt ccggttgtgg aggtgcagcg
     2521 ccttgagatc gtcgagctcg gtggccgcga gtaccggctg ctgcatggca ttggccaccc
     2581 gctgccctga agggatgcgc atggtgaggt tgcggagcag gttccgggtc gccagcgagg
     2641 tcggcgtgcc cggcgactga cccagcagcg cgaagagcac tgaggacaac gtggtgtcga
     2701 tcttcttgtt cggccggacg aggccgtccc cgaagtcgaa gaacgtctgc cagtcgatga
     2761 accggcgcgg ggcgcgatgg ccgccgcgca gatcgtctgg atcggcggcg tcggtcgagg
     2821 tcgggtcgaa gacgagcccg aagaactgct gcgagacgtc ggtcttgctg gtgccgaagt
     2881 tcgcccgata gctcggccgc acctgggagt gtccgaaacg gtacgccgcc accgagaact
     2941 cgaccgggat gaacgggtca tgccgccagg tgtagaacaa cggcccgttg gtcaagacgt
     3001 cgtcggcgat ctcagcgccg acggtcttcg gcaggaactc gtgcaagacc atccactggt
     3061 aatgccaacg aaccacccgc tgcgtctcgg cgaagatctc gtcggaggtg aacgcggcac
     3121 cgagatcttc cttgacgtcg gcgagcaccc ggttgtggaa ccgcaacatg gccagctgca
     3181 gccccgagac catgaggttc tcgtcgttgc gagggtcggc gacgagggcg acgttctggg
     3241 cgttgcgagg caggtcccgt cggtcacagc cgtcgatgga gacggtcgcc gatcctggaa
     3301 tcagctcggt gaggaacgtc gtccggccgc cgtcgaccgt ctggtcgtac agatgcggtg
     3361 agactcccgg accgccgccg tagacgctgt cgaggtcgag ggcggggatc cggaagttgc
     3421 ggatcgactc cggatcctgt gcgcgggcca ggctcgacgt cgggtcgaac gtcatgtcgt
     3481 ggtccaggaa ctggccgagg aacgtcatcc cggccgacag cgtggggttg ttcgggttgt
     3541 gctggctctt cgccgggtcg gtgatcaacg tgatcggatc cgagaggtcg tcttgtgcgt
     3601 ccatcggccc tgccggcgcg ccgagttcgg cgagcgcatc acggatcagc ggcgtgtccg
     3661 ccgagaacgg cggaagggac gggaacaacc ggccgaactt gccgcggtcg tagaacctcg
     3721 aacgtggtgt ctgcgttcct gcgtcggcgt cgccgtgtgc catctgcgca cctccgtggc
     3781 tggtcctcga acccaggagc caccctggcc cgttgagata cgcgtgtcca ggccgcgtta
     3841 acgtcatgga aacatcggac gggcaggatg acccgcatgg ggtaagcagg aagctttccc
     3901 acgaagagcc tgattcagag caacaccgac cgtgcgaaat catctggatg ttgtggcgca
     3961 gactctacag cgtcgctgac gaaacggcgc gcaacatcat cagatccccg cgggcgggga
     4021 tcccgcatgc actccggcgt gcatgcccga acgcaaactg gtcg
//
LOCUS       DATBFZ010000046        11099 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_3313564, whole genome shotgun sequence.
ACCESSION   DATBFZ010000046 DATBFZ010000000
VERSION     DATBFZ010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 11099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11099
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3313564"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1052)
                     /locus_tag="VH419_01380"
     CDS             complement(<1..1052)
                     /locus_tag="VH419_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020576634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY7042298.1"
                     /translation="MTARPAPLAELSADERRKEQHAWYFYDWANSAYVTTVGTVLFSP
                     YLTSVAERAACGRVGTDENPCDTNLHVLGLAISPGSMVFYVVTISTIFSALILPIVGA
                     AADRTGRYKSILAGFAWSGSALAAAMFFVTGTNWQLGALLLVGANLCLGSSLVVYNAI
                     LVQIATPDERDRVSTRGWSLGYLGGGVLLALNLVLVLGHSAFGLDKAMAVRVCLFSAA
                     VWWAGFTIIPFRGLRNLPPADVSPGTGGLARSSFSQLWRTLREMVGFPVLMLFLVASL
                     FINDGVQTVTVASSTYGEKQLGFDTSVLIATILMVQFVAFGGALLFGAAAKVFGAKAG
                     IIVALALWIVIVVIAF"
     gene            complement(1054..1872)
                     /locus_tag="VH419_01385"
     CDS             complement(1054..1872)
                     /locus_tag="VH419_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012921208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HEY7042299.1"
                     /translation="MSRTRSARSRSTHVADYPFFDNGGRPIPFAHRGGALTGDHVGLE
                     NSMAAFEAAIAMGYRHIETDVHSTRDGAVVAFHDTTLDRITDRTGRLDGLDHADLARA
                     LVGGREAIPLLSDILTAWPELRLNIDAKADAAVVPLARCIDQHHAWDRVCVASFSPRR
                     LHQLRKVLDSRVATAYSAFGVGALRLLPSRRLRGWAVHHGQAAQVPPRRGPLEVVTTA
                     FVERAHDLGKQVHVWTIDDAGTMQRLLDLGVDAIMSDRIDVLREVFESRGIWQA"
     gene            1905..2174
                     /locus_tag="VH419_01390"
     CDS             1905..2174
                     /locus_tag="VH419_01390"
                     /inference="COORDINATES: protein motif:HMM:NF024845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HEY7042300.1"
                     /translation="MDAWTCPQCNSEMDERERGGVSVVQCTSCEGLFLRRIDLGIMIE
                     QENDWHVSSGPGTQPIPRIVPGMTPPHEYHSTKKQARSYIDELFG"
     gene            complement(2275..3804)
                     /locus_tag="VH419_01395"
     CDS             complement(2275..3804)
                     /locus_tag="VH419_01395"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020385969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEY7042301.1"
                     /translation="MSEDYLRRIGNLIRDARKHRGYTQTQLADVLGTSQSAVNRIERG
                     HQNLSLEMLARIGEALDSEIVSLGVPGPMHLRVAGGTRLSGSIDVKSSKNAGVALLCA
                     SLLNKGRTTLRRVARIEEVSRILEVLGSIGVKSTWLNEDNDLELIAPDTLDLSGIDQE
                     AARRTRSIIMFLGPLLHDRDSFQLPYAGGCDLGTRTVEPHLAALRPFGLEVKATEGSY
                     HARVDPDVQPVRPIVLTERGDTVTENALLASARHPGVTVIRNASPNYMVQDLCFFLAK
                     LGVRIDGIGTTTLTVHGVPDIDVDVDYAPSEDPIEAMSLLAAAIVTESEITIRRVPIE
                     FLEIELALLEEMGFQYDRTEEYLAGNGHTRLVDLTTRASTLHAPMDKVHPMPFPGLNI
                     DNLPFFAVIAATATGQTMIHDWVYENRAIYLTELTKLGASVTLLDPHRVLVEGPTRWS
                     ATELICPPALRPAVVILLAMLAAKGTSVLRNVYVINRGYEDLADRLNALGAQVESFRD
                     I"
     gene            4051..4821
                     /locus_tag="VH419_01400"
     CDS             4051..4821
                     /locus_tag="VH419_01400"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020386118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEY7042302.1"
                     /translation="MTVLSTPAPAHAEQRHLVALGDSYASGLGTRAYDPDSGSCKRSS
                     YAYPAIDAGRIGATLSFKACAGATTADVRDDQLGPLSAGTTDVTITVGGNDVGFRHVI
                     TECAMPRWASDCNGAIDAARTTIRHPLPRRLNHLYRAVHALAPSANVVVVGYPRVFNG
                     EDCNAGTFFSPAEEARLNATADLLDRTTRARAVAHGFAFVDPRRAFTGHAVCDDPEWI
                     NGLSRPVSESYHPNRKGQRHYANLVSRRLDEAAGATRR"
     gene            complement(4808..6637)
                     /gene="glmS"
                     /locus_tag="VH419_01405"
     CDS             complement(4808..6637)
                     /gene="glmS"
                     /locus_tag="VH419_01405"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020386417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEY7042303.1"
                     /translation="MCGIVGYVGGQTAAPIILEGLGRLEYRGYDSAGLAVLATGGARV
                     VKQAGRVRDLEAILPKRFAGKVGIGHTRWATHGAANDTNAHPHLDASGRVAVVHNGII
                     DNASTLRERLTAAGVELVSETDTEVISHLVAQSDASTLEDAVLDALAQIDGTFGIAVM
                     HADFPDRLVVARNGSPLILGIGEREMHVASDLAALVRYTNQVVHLDDGELASVTATGF
                     TTFTADRQRTDKQATTVDVRAEDYDAGAHAHFMLKEMFEQPQAAERVMRGRLDDRFGT
                     SHLGGLNLDARDTRSIRRVKILGCGSAYYVGQVGAAMIEDMARLPADAEPASEFRYRN
                     PVIEADTLYVAVSQSGETVDTLLAVQEIKRKGGRVVGLVNVVGSAIARECDGGIYLHA
                     GPEVAVASTKALTTMGVGFALLALHLGRVRDLSISDGRRIIEGLRRLPAQIGEILDQD
                     EAIAAIAARLAEAESLFFVGRVRGYPVAREGAQKFKEISYRHAEAYPTSELKHGPLAL
                     ISPEVPTVAVVPADDMADRNLAALHEINARSGPIVVVSHAGVDVGDLADVLTARIDVP
                     VNEPELDPILLTIPLQLLAYHAAVKLGHDVDKPRNLAKSVTVE"
     gene            complement(6646..6906)
                     /locus_tag="VH419_01410"
     CDS             complement(6646..6906)
                     /locus_tag="VH419_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011758026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042304.1"
                     /translation="MPESDLVSRVAATTGLSHGEAARVIADVVAFYSESTEEFVRRRH
                     HHLQTYGMKNPEIFSQITSELRRRVVAAPDLSERQLRRIVYG"
     gene            7093..8340
                     /locus_tag="VH419_01415"
     CDS             7093..8340
                     /locus_tag="VH419_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012918534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042305.1"
                     /translation="MAAPTRHLVGLLLGTEEDWPRAFEALTQRLGVFEYEGEQHVLDC
                     ERVMIEPFNLRTTTRYDVVIDRLAHWYYHPREWLKKVALMDDVYLLNSPFTFQSMEKH
                     SAYCALMRLGMKVPDTVLVPYKNPVDNVRWAYTSERYNRPFNLHALAHDMGYPMYMKP
                     FDGGAWRGVSRIENDEQLDASYDESGEMLMHLQKAVEPYDVFARSLTIGPETMVMNFK
                     PDLPMHNRYAVDHGFLTPETGAEAVTIAQTVNAFFRWEFNSCEMLVRGTDVLPIDYAN
                     ACPDVAVTSLHYYFPWAMSRLVAWTAFCAATDRPARPHIDPTDWFAVADDASLSYDDK
                     LARYQQLADDYFDKERYVAFKEKSLPDLDAMVREWVTSPAFDELLTATVVATYPAHEQ
                     EQFLAHFRGLIDLWIHDQGGVPA"
     gene            8337..9317
                     /locus_tag="VH419_01420"
     CDS             8337..9317
                     /locus_tag="VH419_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017932942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY7042306.1"
                     /translation="MSHPASADGSLEYRPLGSSGLRVSSVGVGCNAFGARIDADRTSE
                     VVEAALEAGVTLFDTADVYGGEPGLSETLLGRALGDRRDDVVIATKFGGDMRGANGPD
                     WGARGSRRYIRRAVDTSLRRLGTDWIDLYQMHNPDPTTPIAETLAALGELVTAGKVRY
                     IGSSNFTGWQVVDADWTAQVAGHPAFVSAQNKYSLYDRSADADLVPACEHIGISLLPY
                     FPLEYGLLTGKYTRGRPAPEGTRLALPTQARRLEGADFGRIEALAAYAQERSVGLLDV
                     AIGGLAAQPFIGSVIAGATSAEQVRTNVRAASWQPTPDDLTALDELTKTG"
     gene            9322..10374
                     /locus_tag="VH419_01425"
     CDS             9322..10374
                     /locus_tag="VH419_01425"
                     /inference="COORDINATES: protein motif:HMM:NF021599.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2332 domain-containing protein"
                     /protein_id="HEY7042307.1"
                     /translation="MAGFDSGATLAERMRAHAADSTHLYGELMRALADDWDAGGPVRS
                     ICRGWEESPQGTVLQLRLLAGLFRIVLTGRAPQLVPYYPCLGGTAPPSQAWPQVREVL
                     VDNVDELHDALAIAPQTNEVGRSGALLLGLFESVRRSGRRRVRLLEVGASAGLNLLVD
                     RFRFEQAAWSFGPADSPLVFRDMFIGDVEPEEFEIVERRGCDLHPVDITTDEGRLRLR
                     SFVWPFQPERHERLMSALEVAAQCPVEVDPFPAGEWLEDQLARGTDGDVLTVVWHSVT
                     RMYWPPEETRRVDATVAAAARRGPVAHVAMEHAPDVMSGADLTLSGIGSDGRPSFVEQ
                     TLATVADHGIPVTVHA"
     gene            complement(10748..>11099)
                     /locus_tag="VH419_01430"
     CDS             complement(10748..>11099)
                     /locus_tag="VH419_01430"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY7042308.1"
                     /translation="TINVAAPDPNVVGHFYEQLLGWNVAIDEPGWMIVRPPGGGVGIS
                     SEFDEAYVAPTWPSEPGKPSQMLHLEIKVDDLEAAVAYATSIGARLAEYQPQEDVRVC
                     LDPAGHPFCLWLGS"
BASE COUNT         1803 a         3750 c         3801 g         1745 t
ORIGIN      
        1 aagaacgcga tcacgacgat cacgatccac agcgcgagcg ccacgatgat gccggccttt
       61 gcgccgaaga ccttcgccgc cgctccgaag agcagcgccc cgccgaaggc gacgaactgc
      121 accatgagga tcgtggcgat gagcacggag gtgtcgaagc ccagctgctt ctcgccgtac
      181 gtcgaagacg ccacggtcac ggtctggacg ccgtcgttga tgaacagcga cgccaccagg
      241 aacagcatca acaccgggaa cccgaccatc tcgcgcagcg tccgccagag ctggctgaag
      301 ctggaccgag ccagcccccc ggtcccagga gagacgtcgg ccggtggcag gttccggaga
      361 ccccggaagg ggatgatcgt gaagcccgcc caccagaccg ccgcgctgaa caggcagacc
      421 cgcaccgcca tcgccttgtc cagcccgaac gcgctgtgcc cgagcaccag cacgaggttg
      481 agggccagca ggacgccgcc gccgaggtag cccagcgacc atcctcgcgt cgatacacgg
      541 tcccgctcgt cgggtgtcgc gatctgcacc aggatcgcgt tgtagacgac caggctggag
      601 ccgagacaca ggttcgcccc gacgagcagg agcgcaccga gctgccagtt cgtgccggtg
      661 acgaagaaca tcgctgcggc caatgcgctg cccgaccacg cgaacccggc caggatcgac
      721 ttgtaccgac cggtccggtc ggccgccgcg cccacgatcg gcaggatcag cgcggagaag
      781 atcgtcgaga tcgtgacgac gtagaacacc atcgagcctg gggagatcgc gagacccagc
      841 acgtgcaggt tggtgtcgca ggggttctcg tcggtaccga cccgaccgca ggccgcgcgc
      901 tcggcgaccg acgtcagata gggggagaac aacacggtgc cgacggtggt gacgtacgcc
      961 gagttcgccc agtcgtagaa gtaccaggcg tgctgctcct tgcgtcgctc gtcggccgac
     1021 agctcggcga gcggcgcagg gcgggccgtc atctcacgcc tgccagatac cgcgggactc
     1081 gaagacctcg cggagcacgt cgatccggtc cgacatgatc gcgtcgacgc cgaggtcgag
     1141 cagccgttgc atggttccag catcgtcgat ggtccaaacg tggacctgct tcccgaggtc
     1201 gtgggcgcgt tcgacgaacg cggtcgtcac gacctccagc ggccctctgc ggggcggcac
     1261 ctgcgccgcc tgtccgtggt ggaccgccca tccccgcagc cgtcggctcg ggaggagtcg
     1321 aagggcgccc acaccgaagg cggagtacgc cgttgccacc ctcgaatcga gcaccttcct
     1381 cagctggtgc agccggcgag gagagaagga tgcgacacac acgcggtccc acgcgtgatg
     1441 ctggtcgatg cagcgcgcca gcggtacgac ggccgcgtcg gccttggcgt cgatgttcag
     1501 tcgaagctcc ggccaggcgg tgaggatgtc actcagcagt gggatggcct cccggccacc
     1561 gaccagagca cgggcaaggt cggcgtggtc gaggccgtcc agccgtccgg tccggtcggt
     1621 gatgcggtcc agggtcgtgt cgtggaacgc gaccacggcg ccgtcgcggg tggagtgcac
     1681 gtcggtctcg atgtgccggt agcccatggc gatggcggcc tcgaatgcgg ccatcgagtt
     1741 ctccaacccg acgtggtcgc cggtgagtgc gccgccgcga tgggcgaacg ggatcggtcg
     1801 tccaccgttg tcgaagaagg ggtagtcggc aacgtgcgtg ctgcgacttc gggcggaccg
     1861 tgtcctgctc acagcaccga agtatgacgg gttacggttg ccccatggac gcttggacct
     1921 gcccgcaatg caactccgaa atggacgagc gagaacgagg cggtgtcagc gtcgtacagt
     1981 gcacctcctg cgaagggctc ttcctgcgtc ggatcgacct cggcatcatg atcgagcagg
     2041 agaacgactg gcacgtgtcg tccgggccgg gcacccagcc gattccgcgc atcgtccccg
     2101 gcatgacacc gccccacgag taccactcca ccaagaagca ggcgcgcagc tacatcgacg
     2161 agctcttcgg ctgatccgcc ctcgggtggc tcccgcgaat tgcgggaaaa gtcaccctca
     2221 gatcgccgtc tggacctgcg ttttcccgca attcgcggca gcccggcagt tgggtcagat
     2281 gtcgcggaag gactcgacct gggcgccgag cgcgttcagc cgatcggcca ggtcttcgta
     2341 gccacggttg atgacgtaga cgttgcgcag caccgacgtg cccttcgccg ccagcatcgc
     2401 cagcaggatg accacggccg gccgcagcgc gggtgggcag atgagctccg tcgccgacca
     2461 gcgggtcggg ccctcgacga ggacgcggtg cgggtcgagc aaggtgacgc tcgcgccgag
     2521 cttggtgagc tcggtcaggt agatcgcacg gttctcgtag acccagtcgt ggatcatcgt
     2581 ctgcccggtg gcggtcgccg cgatgaccgc gaagaacggc aggttgtcga tgttgagccc
     2641 cgggaacggc atcgggtgga ccttgtccat cggcgcgtgc agcgtcgatg cgcgggtggt
     2701 gaggtcgacc agcctcgtgt ggccgttgcc ggcgaggtac tcctcggtgc ggtcgtactg
     2761 gaaccccatc tcctccagca gcgcgagctc gatctcgagg aactcgatcg gcactcggcg
     2821 gatcgtgatc tcggactcgg tgacgatcgc ggcggcgagc aggctcatcg cctcgatcgg
     2881 gtcctccgac ggtgcgtagt cgacatcgac gtcgatgtcc gggaccccgt gcacggtcag
     2941 cgtcgtcgta ccgatgccgt cgatgcgcac ccccagcttg gcgaggaaga agcacaggtc
     3001 ttgaaccatg tagttcgggc tcgcgttgcg gatcacggtc acgccagggt ggcgggccga
     3061 ggcgaggagg gcgttctcgg tgacggtgtc gccgcgctcg gtgagcacga tgggccggac
     3121 cggctgcaca tcggggtcga cccgcgcgtg gtagctcccc tcggtggcct tgacctcgag
     3181 gccgaacggc cgcagcgccg ccaggtgcgg ttcgacggtg cgggtgccca ggtcgcatcc
     3241 acccgcgtac ggaagctgga agctgtcacg gtcgtgcagc agcgggccga ggaacatgat
     3301 gatgctgcga gtacgccgag cagcctcctg gtcgatgccc gacaggtcga gcgtgtccgg
     3361 ggcgatcagc tcgaggtcgt tgtcctcgtt cagccacgtc gacttcacac cgatgctgcc
     3421 gagcacctcg aggatgcgac tgacctcctc gatacgggcg accctccgca gcgtggtgcg
     3481 tcccttgttg agcagcgagg cacacagcag ggcgacaccg gcgttcttgc tcgacttcac
     3541 gtcgatcgag ccggagagcc gggtcccgcc ggccacccgc aggtgcatcg gtcccggcac
     3601 cccgagcgag acgatctcgg agtcgagcgc ctcgccgatc cgggccagca tctccaggct
     3661 gaggttctga tggccacgct cgatccggtt gacggcgctc tggctggtgc cgaggacatc
     3721 ggcaagctgg gtctgggtgt agccgcggtg cttgcgagca tcccggatga gattgccgat
     3781 gcgacgcaag tagtcctcac tcatgactcg acggtatctc acatgtgaga taccgcaaat
     3841 gcggcgcgcg gtgtctccac agattgccgg tccaggcttg caggcgagcg tcgccggcga
     3901 caaggtgaaa gagatcacgt catgcacgca tcgaatcaga ccctgaatca gaccctgagt
     3961 cacaccctga ggcacgtcct gagtcgccca ccgagccgaa cgcgcaccgc cgcagtcgct
     4021 gctgccgtcg cgatcgtctg tgccgtcgcg gtgaccgtgc tgagtacgcc ggcccccgcc
     4081 cacgcggagc agcggcacct ggtcgcgttg ggggactcct acgcctccgg actcgggacc
     4141 cgcgcctacg accccgactc aggctcgtgc aagcggtcgt cctacgccta tccggccatc
     4201 gacgccggca ggatcggcgc gacgctgtcg ttcaaggcct gcgcgggggc caccacggcc
     4261 gacgtgcgtg acgaccagct cggcccgctg tcggccggca ccactgacgt caccatcacc
     4321 gtgggcggca acgacgtcgg tttccggcac gtcatcaccg agtgcgcgat gccgcgctgg
     4381 gcgtcggact gcaacggggc catcgacgct gcccggacga cgatccgcca cccgctgcca
     4441 cgccggctca accacctgta ccgcgcggtc cacgctctgg ctccatcggc gaacgtggtc
     4501 gtggtcggct acccacgggt cttcaacggt gaggactgca atgccgggac gttcttcagc
     4561 ccggccgagg aggccaggtt gaacgcgacg gcagatctgc tcgaccgaac gacccgggct
     4621 cgcgcggtgg cccacggctt cgcgttcgtc gacccgaggc gcgcgttcac cggtcatgcg
     4681 gtgtgtgatg acccggaatg gatcaacggt ctgtcccgcc cggtgtccga gtcgtaccac
     4741 ccgaaccgca agggccagcg acactacgcg aacctcgtct cccgccgcct cgacgaggcc
     4801 gccggggcta ctcgacggtg acggacttgg cgaggttgcg tggcttgtcg acgtcgtggc
     4861 ccagcttgac ggccgcgtgg taggccaaca gctggagcgg gatcgtcagc aggatcgggt
     4921 cgagctcggg ctcgttcaca ggtacgtcga tccgggcggt cagcacgtcc gcgagatcac
     4981 cgacgtcgac acccgcgtgg ctgacgacga cgatcggtcc gctgcgagcg ttgatctcgt
     5041 gcagcgccgc gaggttccgg tcggccatgt cgtcggccgg cacgaccgcc accgtcggca
     5101 cctctgggct gatcagcgcg agtggtccgt gcttgagctc cgacgtcgga taggcctcgg
     5161 cgtgccggta cgagatctcc ttgaacttct gcgctccctc gcgcgcgacg gggtagccgc
     5221 gtacccggcc gacgaagaac aggctctctg cttcggccag ccgagcggcg atcgcggcga
     5281 tcgcctcgtc ctggtcgagg atctcgccga tctgggcggg gagtctccgg aggccctcga
     5341 tgatccttcg tccgtcggag atcgacaggt cccggacccg gccgaggtgc agcgccagca
     5401 acgcgaaccc gacacccatc gtggtgagcg ccttggtgga cgccacggcg acctcaggtc
     5461 ccgcatgcag gtagatcccg ccgtcgcact cacgggcgat ggccgaaccg acgacgttga
     5521 cgagcccgac cacgcggccg cccttgcgct tgatctcctg gacagcgagc agcgtgtcga
     5581 cggtctcacc ggactggctg accgcgacgt acagcgtgtc ggcctcgatc accgggttcc
     5641 ggtagcggaa ctccgaggca ggttcggcgt cggccggcag ccgggccatg tcctcgatca
     5701 tcgcggcgcc gacctggccg acgtagtacg ccgacccgca gccgaggatc ttgacgcgcc
     5761 ggatcgatct ggtgtcgcgg gcgtccaggt tcaggccgcc gaggtgggat gtcccgaacc
     5821 ggtcgtcgag gcggcctcgc atcacccgct cggccgcctg cggctgctcg aacatctcct
     5881 tgagcatgaa gtgcgcgtgc gcgccggcgt cgtagtcctc ggcacgcacg tcgaccgtgg
     5941 tggcctgctt gtcggtccgc tgccggtcgg ccgtgaacgt ggtgaacccg gtcgcggtga
     6001 ctgacgccag ctcgccgtcg tcgagatgca cgacctggtt cgtgtagcgg accagcgcgg
     6061 cgaggtcgct ggcgacgtgc atctcgcgct caccgatccc gaggatcagt ggggagccgt
     6121 tgcgagcgac gacgagtcgg tcggggaagt ccgcgtgcat cacggcgatg ccgaacgtcc
     6181 cgtcgatctg cgccagcgcg tcgagtacgg cgtcctcgag agtgctcgcg tcggactgcg
     6241 cgaccaggtg gctgatcacc tcggtgtcgg tctcggacac cagctcgacg ccggcggcgg
     6301 tgagccgttc ccgcagcgtg ctggcgttgt cgatgatccc gttgtggacg actgcgaccc
     6361 ggccgctcgc gtccagatgg gggtgggcgt tcgtgtcatt ggccgcaccg tgcgtcgccc
     6421 atcgggtgtg accgattccg accttgccgg cgaaccgctt gggcagaatc gcctcgaggt
     6481 cacggacacg gccggcctgc ttcacgaccc tggcaccgcc cgtcgccagc accgcaagcc
     6541 cggcagagtc gtagccgcgg tactcgagcc ggccgagccc ttccaagatg atgggggccg
     6601 cggtttgacc gccgacgtaa cccacgatgc cgcacatgac gctcctcagc cgtagacgat
     6661 gcgccgcagc tggcgctcgg acaggtccgg ggcggcgacc acccgtcgcc tgagctcgct
     6721 ggtgatctgc gagaagatct ccgggttctt catgccgtag gtctgcaggt ggtggtgccg
     6781 gcgccggacg aactcctcgg tggactcgga gtagaaggcg accacgtcgg cgatcacccg
     6841 ggccgcctcg ccgtgcgaca gccccgtggt cgcggccacg cgactcacca gatcagactc
     6901 gggcatggcc acgatggtga cacctcatcg gaacaaagtc cacgcgcctg cccgaaatcg
     6961 ggcaggaaat cgctgcagca ccgcgcgaag tcacctgttg cgctgtgtgg tgtctgcgag
     7021 ttggctgagg cgtggggcgc gaaccgtcca gcaacgcgcc cggccgacgc tcgtccgagg
     7081 agaggacacg acatggctgc acccacgcga cacctcgtcg ggctgttgct cgggacggag
     7141 gaggactggc cgcgggcctt cgaggcgctg acccagcggc tcggggtctt cgagtacgag
     7201 ggggagcagc acgtgctcga ctgcgaacgg gtcatgatcg agccgttcaa cctgcgcacg
     7261 acgacgcgct acgacgtggt catcgaccgg ctggcgcact ggtactacca cccgcgtgaa
     7321 tggctgaaga aggtcgcctt gatggacgac gtgtacctgc tgaactcgcc gttcacgttc
     7381 cagtcgatgg agaagcactc ggcgtactgc gcgctgatgc ggctcggcat gaaggtgccg
     7441 gacaccgtgc tcgtcccgta caagaacccg gtcgacaacg tccgttgggc ctacacctcg
     7501 gaacgctaca accggccctt caacctgcat gcgctcgccc atgacatggg ctacccgatg
     7561 tacatgaagc ccttcgacgg aggcgcctgg cgcggcgtct ccaggatcga gaacgacgag
     7621 cagctcgacg cgtcgtacga cgagtccggc gagatgctca tgcacctgca gaaggccgtc
     7681 gagccgtacg acgtcttcgc ccgctcactg acgatcgggc cggagaccat ggtgatgaac
     7741 ttcaagccgg acctgccgat gcacaaccgc tacgccgtcg accacggctt cctcacgccc
     7801 gagaccggcg cggaagcggt gaccatcgca cagaccgtga acgcgttctt ccgctgggag
     7861 ttcaactcct gcgagatgct cgtccgcggc accgacgtgc tcccgatcga ctacgcgaac
     7921 gcctgcccgg acgtcgcggt cacgtcgctg cactactact tcccgtgggc aatgtcccgg
     7981 ctggtggcct ggacggcgtt ctgtgccgcg accgaccgac cggcccgtcc gcacatcgac
     8041 ccgacggact ggttcgccgt ggccgacgat gcgagcctgt cgtacgacga caagctcgcg
     8101 cgctaccaac agctggcgga tgactacttc gacaaggagc gatacgtcgc gttcaaggag
     8161 aagtcgctgc ccgacctcga cgcgatggtg cgggagtggg tgacctcacc ggcgttcgac
     8221 gagctgctga cggcgacggt cgtcgcgacg tacccagcgc acgagcagga gcagttcctg
     8281 gcccacttcc gcggcctgat cgacctctgg atccatgacc agggcggcgt acccgcgtga
     8341 gccatccggc atcggccgac ggatcgctcg agtatcggcc actggggagc tccggcctca
     8401 gggtgagcag cgtcggtgtc gggtgcaacg cgttcggcgc gcgcatcgac gccgatcgga
     8461 ccagcgaggt ggtcgaagcc gccctcgagg cgggcgtgac gctgttcgac accgccgacg
     8521 tgtacggcgg ggagcccggc ttgtccgaga cgctgctcgg ccgcgcgctg ggcgatcgtc
     8581 gcgacgacgt ggtgatcgcg accaagttcg gtggcgacat gcgtggtgcg aacggtccgg
     8641 actggggggc gcgtggttcg cgccgctaca tccgtcgcgc ggtcgacacc agcctgcgcc
     8701 gactcggaac cgactggatc gacctctacc agatgcacaa cccggacccc accacgccga
     8761 tcgcggagac cctggcggcc ctgggagaac tcgtcaccgc tggcaaggtg cgctacatcg
     8821 ggtcgtcgaa cttcaccggc tggcaggtcg tcgatgccga ctggacggca caggtcgccg
     8881 gacacccggc gttcgtgagc gcacagaaca agtactcgct ctacgaccgc tccgccgatg
     8941 ccgacctggt ccctgcgtgt gagcacatcg gcataagcct gctcccgtac tttccgttgg
     9001 agtacgggct gctcaccggg aagtacaccc gcggccggcc ggctccggaa ggcaccagac
     9061 tcgcgttgcc gacgcaggcg agacgactcg aaggtgccga cttcggccgc atcgaggcgt
     9121 tggcggcgta cgcgcaggag cggtcggtcg gtctcctcga cgtcgcgatc ggtggcctgg
     9181 ccgcccagcc gttcatcggg tcggtgatcg ctggtgcgac cagcgcggag caggtccgga
     9241 ccaacgtccg ggccgcgtcc tggcagccga cccctgacga tctgacggcg ttggacgagc
     9301 tgaccaagac cggctgacgg cgtggccggg ttcgacagcg gtgcaacgct cgccgagcgg
     9361 atgcgcgccc acgccgcaga cagcacgcat ctgtatggcg agctgatgcg tgccttggcc
     9421 gacgactggg acgccggtgg cccggtccgg tcgatctgcc gtggctggga ggagagccca
     9481 cagggcaccg ttctccagct ccgactgttg gccgggttgt tccggatcgt gctgaccgga
     9541 cgggcacccc agctggtgcc gtactacccc tgcctcggcg ggacggcgcc accgagccag
     9601 gcatggccac aggtgcgcga ggtgctcgtc gacaacgtgg acgagctgca cgacgcattg
     9661 gccatcgccc cgcagaccaa cgaggtcggc cgctcgggcg cgctgctgct cggtctattc
     9721 gagtccgtac gtcggagtgg gcgccgacgc gtcaggctgc tcgaggtcgg agcatctgcc
     9781 ggcctcaacc tgctcgtcga caggtttcgc ttcgagcagg cggcatggtc cttcgggccg
     9841 gcggactccc cgctggtctt ccgcgacatg ttcatcggcg atgtcgagcc cgaggagttc
     9901 gagatcgtcg aacgacgagg ctgcgacctg cacccggtgg acatcacgac cgacgagggc
     9961 cggctgcggc tgcggtcgtt cgtctggccg ttccagcccg agcgccatga acggctgatg
    10021 agcgcgttgg aggtcgccgc gcagtgcccg gtggaggtgg acccgtttcc ggcgggggag
    10081 tggctcgagg accagctggc caggggaacc gacggcgatg tcctcaccgt cgtctggcac
    10141 tcggtgaccc gaatgtactg gccgccggag gaaacccgtc gggtcgacgc cacggtggct
    10201 gccgccgcac gtcgcgggcc cgtcgcgcac gtggcgatgg agcacgcccc cgacgtcatg
    10261 agcggcgccg atctgacgtt gtccgggatc ggttccgacg gccgacccag ttttgtggag
    10321 cagacccttg ccaccgtcgc cgaccacggc atccccgtca ccgtccacgc atgaacctgc
    10381 cactctccct ccatggtggt ttgcctgggt agccatgcaa acggatgggt agccatgcag
    10441 atctgccctg tgaggggtgg gtgtgcctgg gtacccatgc aaaccgccgc cggcgaccga
    10501 cggatgtccg aaacccgcca gtcacacgtc agtcaacagg aacggaacgc cggccgagcg
    10561 gcttagctgg cacgaacgtc tcccgctcgt cgctgccatc ccacctcagc cagaggccga
    10621 cggaccgcgg gacgaccacg gccgcctcat cgcgaccatg tccgtccggc gagtctcgag
    10681 gagtgccggt ccttcgaatg ctccagtacc cgacggcccg ggacgccgtg gcgggggagg
    10741 agggtggtca gctgccgagc cagaggcaga acggatgtcc ggcggggtcg aggcagacgc
    10801 ggacatcttc ttgcggctgg tactccgcca gccgagctcc gatcgacgtg gcgtaggcga
    10861 ccgcggcttc gaggtcgtcg accttgatct ccaggtgcag catctggctc ggcttgccgg
    10921 gctccgacgg ccatgtcggc gcgacgtacg cctcgtcgaa ctcgctcgag atgccgacac
    10981 cgccgccagg tgggcggacg atcatccacc ccggctcgtc gatggcgacg ttccacccca
    11041 acagctgctc gtagaagtga ccgacgacgt tgggatcggg cgccgcgacg ttgatcgtg
//
LOCUS       DATBFZ010000047         3129 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_1017516, whole genome shotgun sequence.
ACCESSION   DATBFZ010000047 DATBFZ010000000
VERSION     DATBFZ010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 3129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3129
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1017516"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..601)
                     /locus_tag="VH419_01435"
     CDS             complement(116..601)
                     /locus_tag="VH419_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042309.1"
                     /translation="MNRLRKISRPIAIGAALVAPMLATVPSHASTSFQGCTLTPERPA
                     YAGFNNASNIPVINYKFTIDCDQGLSAEVKQVRMEQDLVSREGDAPDDQTGTFSKVYD
                     FPNGGVHHVTVQAPLPNTGPSNEGATEEVYQKVKMRVTSTTVTGPWTPFELTDVRSIH
                     R"
     gene            complement(857..1969)
                     /locus_tag="VH419_01440"
     CDS             complement(857..1969)
                     /locus_tag="VH419_01440"
                     /inference="COORDINATES: protein motif:HMM:NF013559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallopeptidase"
                     /protein_id="HEY7042310.1"
                     /translation="MPRKRLKVCYIAPEGGSPHVVPRKSGQQVAALERVDNVRVTPLA
                     SGIFGPQRAAVEKLTLWDNGRTLRVKFLDGDPDVQTKVTQIAKEWEAVANITLDFVTS
                     GASEVRISFLQKGFSWSTVGTDALTVSSTQPTMNYGWLTASTALREYQRVVRHEFGHA
                     LGMIHEHQNPAAAGVIPWDKPKVYAYYAQQGWTKSDVDHNIFATYAADATNFTAFDPT
                     SIMEYAIPDELTIGSYAIGWNTELSAQDRSFMAKQYPMASPGTVELEIGGPRYEADLS
                     VGAEVDTYHFQVGTPATFIMTTEGPTDTVLTVQGPNDPGAIVAWDDDRGAGLNARIVR
                     KLYEGDYWLTVKHKDPGNVPPDSTGTYTVGLKQRKS"
     gene            complement(2190..>3129)
                     /locus_tag="VH419_01445"
     CDS             complement(2190..>3129)
                     /locus_tag="VH419_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042311.1"
                     /translation="RMRLTASLKRRDDVVGERSWHGSPDVRPRIVTDPTIPPPDLPWQ
                     FGLLPIAGDNVRRFQAALKSRTQDPRIRVNGLFDAYFDEIARQLLPPDPTLPPGAPPV
                     PPGRVTAGIWGAVMQPPHGSAEAWGIATPTETDLHDSTPTLTEGTLLPASVNLRALPC
                     KVEVVVHRRGLTDISGGDVLVTLLRWIDPASPQTANPASSLTWFGGPVPWGPAVDEVL
                     NSNTGTTSQTFGDGWSFVGTTDETRRKPLVGQHLDNTHSGVASFDLNLTGLAGQVVLL
                     VAVIRADGDSSIQSLPIKDLALTNPHVAVRSIRVTV"
BASE COUNT          461 a         1022 c         1087 g          559 t
ORIGIN      
        1 ggcggcttag ggtcgagcct gccaagaccg actcgtccgc tggtcgaact tgtcgagacc
       61 gaggaccgtt gaagcgatcc gggtctcgac aagctcgacc accgaagtcc ccggctcagc
      121 ggtggatcga gcggacgtcg gtcagctcga acggcgtcca tggcccggtc accgtggtgc
      181 tggtgacgcg catcttgacc ttctggtaga cctcttccgt cgcgccctcg ttggagggac
      241 cggtgttggg cagcggcgcc tgcacggtca cgtggtgcac accgccgttc gggaagtcgt
      301 agaccttcga gaaggtgccc gtctggtcgt cgggggcgtc gccctcgcgg ctcaccaggt
      361 cctgctccat ccggacctgc ttcacttccg cagacaggcc ctggtcgcag tcgatggtga
      421 acttgtagtt gatgaccggg atgttgctcg cgttgttgaa gccggcgtac gccggtcgct
      481 ccggcgtgag cgtgcaaccc tggaagctgg tcgacgcgtg actcggcacg gtcgcgagca
      541 tcggagctac cagggcggcg ccgatggcga tcggccggct gatcttgcgc aatctgttca
      601 tgattcctct tcctgacatg cgacccgagt ggtcgccgtg gtggtgacgc cgcgaggtcc
      661 gcgacgtggg aatcaccgtg cccgacgccc atcagcatgg cgtcagtacg agatctgcta
      721 ccggtcggcc agacgccgct cggggctccg cgaattgcgg gaaacgtccg ggtctgcccg
      781 gcgtgtccac cgcaactttc ccgcaattcg ccggagccca gggcttgggg tgtggtcggg
      841 gttggcggga ggggagctag gacttgcgtt gtttgaggcc gaccgtgtag gtgccggtgc
      901 tgtccggggg aacgttgccg gggtccttgt gcttgacggt caaccagtag tcgccctcgt
      961 acagcttccg gacgatccgg gcattcaggc cggcgccgcg gtcgtcgtcc catgccacga
     1021 tcgcacccgg gtcgttcggg ccctggacgg tcaacaccgt gtcggtcggg ccctcggtgg
     1081 tcatgatgaa cgtcgccggc gtgccgacct ggaagtggta cgtgtcgacc tccgcgccga
     1141 ccgacaggtc cgcctcgtag cgcgggccgc cgatctcgag ctcgaccgtc cccggtgagg
     1201 ccatcgggta ctgcttcgcc atgaacgacc ggtcctgcgc ggagagctcg gtgttccacc
     1261 cgatggcgta cgacccgatc gtcagctcgt ccgggatcgc gtactccatg atcgacgtcg
     1321 ggtcgaaggc cgtgaagttg gtggcgtcgg cggcgtacgt cgcgaagatg ttgtggtcga
     1381 cgtcggactt cgtccacccc tgctgcgcgt agtacgcata gaccttcggc ttgtcccagg
     1441 ggatgacacc ggccgctgcg gggttctggt gctcgtggat catcccgagg gcgtggccga
     1501 actcgtgccg gacgacccgc tggtactccc gcaaagccgt gctcgccgtc agccatccgt
     1561 agttcatcgt cggctgcgtc gacgacaccg tcagtgcgtc ggtaccgacc gtggaccacg
     1621 agaacccctt ctggagaaac gagatccgga cctccgacgc gccgctcgtc acgaagtcga
     1681 gggtgatgtt tgccaccgcc tcccactcct tggcgatctg ggtgaccttc gtctgtacgt
     1741 cgggatcgcc gtcgaggaac ttcacccgca gcgtccgacc gttgtcccac agcgtcagct
     1801 tctccaccgc ggcgcgctgt ggtccgaaga tcccggatgc cagtggtgtg acccgaacgt
     1861 tgtcgacgcg ctccagcgcc gccacctgct ggccgctctt gcgcggcacc acgtgcgggg
     1921 agccgccctc cggcgcgatg tagcagacct tcagtcgctt cctcggcatc gcgactcctt
     1981 ccgtcttgcg ctggtcgccg aacccgctga tcgagcttgt cgagatcaag ggggtggtct
     2041 cgacaggctc gaccgccgga ggtccgccga tcgccgaacc cgctgatcaa gctctttcga
     2101 ccgaggtggc aatcgaatgg cgacacgccg gcacattgca acaaaagttg ccacctcggc
     2161 ggggtccaac ggggctacgt cggcgggggt cataccgtca cgcggatgga gcggacggcg
     2221 acgtgggggt tcgtgagggc caggtccttg atcgggagcg actggatcga gctgtcgccg
     2281 tcggcgcgga tcaccgccac cagcagcacc acctgcccgg ccagcccggt cagattcagg
     2341 tcgaagctcg ccaccccgga gtgcgtgttg tcgaggtgct gtccgacgag cggtttacgc
     2401 cgggtttcgt cggtcgtacc gacgaaggac cagccgtcac cgaacgtctg cgaggtcgtg
     2461 ccggtgttgg agttgagcac ctcgtcgacg gccggacccc atggcacagg cccgccgaac
     2521 cacgtgagcg acgaggccgg gttggccgtc tgcggcgagg caggatcgat ccagcggagc
     2581 agcgtgacga ggacgtcgcc gccggagatg tcggtcaggc cgcgccgatg cacgacgacc
     2641 tcgaccttgc acggcaacgc tcgcaggttg accgaggcgg gcagcagcgt gccttccgtc
     2701 aaggttggcg tcgagtcgtg caggtcggtc tcggtcggcg tcgcgatgcc ccaggcctcg
     2761 gccgacccgt gcggcggctg catgacggca ccccagatgc ctgccgtcac ccttcctgga
     2821 ggtaccgggg gagcgcccgg cggcagcgtc gggtccggcg gcagcagctg gcgggcgatc
     2881 tcgtcgaagt aggcgtcgaa caacccgttc acgcgaatcc gcggatcttg ggtgcgcgac
     2941 ttcaacgccg cctggaagcg tcggacgttg tcacccgcga tcggcagcag gccgaactgc
     3001 cagggcaggt cgggcggcgg gatcgtgggg tcggtcacga ttcgcggtcg cacgtcgggg
     3061 ctgccgtgcc acgagcgttc gccgacgacg tcgtcacgtc gcttgaggct cgcggtgaga
     3121 cgcatccgc
//
LOCUS       DATBFZ010000048         2215 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_2657798, whole genome shotgun sequence.
ACCESSION   DATBFZ010000048 DATBFZ010000000
VERSION     DATBFZ010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2657798"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(159..1211)
                     /locus_tag="VH419_01450"
     CDS             complement(159..1211)
                     /locus_tag="VH419_01450"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY7042312.1"
                     /translation="MSRTLVITNDFPPRRGGIENFVYSICAAMNPEEVVVYTSRTPGD
                     DEFDRTLAFPVLRDRARVLLPTPRVARRAQQIAREHGCDRVVFGAAAPLGLLARGLRN
                     RTGATHLAAITHGHEVWWAKVPVARQALRRIADEVDVVTYVSEFCRERIAPALSPAAR
                     TRMQRLSPTVDTNRFHPGVDGSQWRRRLGIGPDIPVVLSASRLVRRKGQDVLIKAWPG
                     VRCTFPDARLVIAGDGPSRRRLHRLATSVGVAGSVCFIGSQFWEEMPAIYAMADMFVL
                     PCRTRLWGLEVEAFGMVFLEAASAGLPVVAGDSGGAPQTAQSIGGTVVDPCSSFAVAT
                     EIARKLDGRDRAIRHS"
     gene            complement(1208..>2215)
                     /locus_tag="VH419_01455"
     CDS             complement(1208..>2215)
                     /locus_tag="VH419_01455"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEY7042313.1"
                     /translation="RLSGAVRRRVPWWPAQVGVLVATVLAFQLVIALPFAVWSSAVSR
                     YYGLTTQDWAGWLQDLLLGWSIALLGTALCMLGGVWLARRSPERWWIPAASGAAGLVF
                     VVSFAYPVLVEPLFNRFSSMPASPLRTQLLEYAARDGIDVSDVLVADASRRTTAYNAY
                     VSGFGSTKRLVLYDNLLAGGRTDQVKVVVAHELGHVRNHDVLIGTTLGAAAAATGVVG
                     LFLLLRRSWWRRRVGADSAGDPAVVPLVLSLVVLVGFLALPVQNLVSRHVEARADLHS
                     LDLTQNPTAFIGLQQRLAVTNISHLTPNPILAWWFDDHPTAMDRIAMAVAWADEHAGE
                     P"
BASE COUNT          427 a          733 c          741 g          314 t
ORIGIN      
        1 gatcaagagc gaaggcacga tcaccaccac gtgtcccgct gacgagtgac gctcgccgta
       61 gggccaggga actgcggtgg ccatgacgaa ggagttcccg agaagtccgt ccgcgaagga
      121 gggccgaggt gaaggggctt cgcaatcagg acggcccgtc agctgtggcg gatggctcgg
      181 tctcgaccgt cgagttttct cgcaatctcg gtcgcgacgg cgaacgacga gcaagggtcg
      241 accacagttc caccgatgga ttgcgcggtc tgcggggcac cacctgaatc gccggcgacc
      301 accggcaagc cagcggatgc ggcctcgagg aacaccatcc cgaatgcttc gacctccagc
      361 ccccagaggc gagtccgaca cggtagtacg aacatgtccg ccatcgcata gatcgcaggc
      421 atctcctccc agaactggct accgatgaag cacacggaac cggctacgcc cacggaggtg
      481 gcaagtcggt ggaggcgtct acgggacggg ccatctcccg cgatgaccag ccgtgcatca
      541 gggaacgtac agcgcacacc gggccatgcc ttgatgagga cgtcctgccc cttgcggcgt
      601 accagtcgcg acgctgacag caccaccggg atgtctggcc cgatgccgag tcggcgacgc
      661 cattgggaac cgtcgactcc cgggtggaac cggttggtgt cgaccgtcgg cgacaagcgc
      721 tgcattctgg ttcgcgccgc cggcgaaagg gcgggggcga tgcgctctct gcagaactcg
      781 ctgacgtagg tgaccacgtc gacttcgtcg gcgattcgac gcaaggcctg tcgagccaca
      841 ggcaccttgg cccaccacac ttcgtggcca tgtgtgatgg cagccaggtg tgtggcgccg
      901 gtcctgttcc gcaaaccgcg tgccaacaac ccgagcggcg cggctgcccc gaacacgaca
      961 cggtcacagc cgtgctcgcg agcgatctgc tgggctcttc gagccactcg tggcgtcggc
     1021 agcagaaccc tggcccggtc gcgaaggacg ggaaaggcga gtgtccggtc gaactcgtcg
     1081 tcgccagggg ttcgagacgt gtagacaacg acctcttcgg gattcatggc ggcgcagatc
     1141 gagtagacga agttctcgat gccgccgcgg cgcggcggga agtcgttggt gatgacgagc
     1201 gtcctgctca cggctcgccc gcatgctcat ccgcccaagc aacggccatc gcaatccggt
     1261 ccatggctgt cgggtgatcg tcgaaccacc aggccaggat cgggttcggc gtgaggtggg
     1321 agatgttcgt gacagcgagt cgctgttgca ggccgatgaa cgccgtcggg ttctgggtga
     1381 gatcgagcga gtgcaggtcc gcgcgcgcct cgacgtggcg actcaccagg ttctgcaccg
     1441 gcagcgcgag gaagccgacg agtacgacca gcgacagcac gagcggcaca actgcaggat
     1501 cgccggccga gtcggctccg acacggcgtc gccaccacga acgacgtaac aacaggaaga
     1561 gccccacaac tccggtggcg gccgccgccg cgccgagggt cgtcccgatg aggacatcgt
     1621 ggttgcgtac atgaccgagc tcgtgcgcga ccacgacctt cacctggtcg gtccgaccgc
     1681 ctgccagcag gttgtcgtag aggacgagcc gcttcgtcga cccgaagccc gagacgtagg
     1741 cgttgtatgc cgtcgtacgc cgcgacgcgt cggctacgag cacgtccgag acgtcgatcc
     1801 cgtcccgcgc ggcgtactcg agcagctggg tgcgcaacgg cgacgccggc atcgaggaga
     1861 aacggttgaa caacggctcc acgaggaccg ggtaggcgaa cgacacgacg aagaccaaac
     1921 cagcggctcc cgaggcagct ggtatccacc agcgctccgg cgagcgccgt gccagccaca
     1981 ccccacccag catgcacagg gccgtgccca gcagcgcgat ggaccagccg aggaggagat
     2041 cctgaagcca tccggcccag tcctgtgtgg tcagcccgta gtaccgcgac accgcgctgc
     2101 tccagacggc gaacggcagg gcgatgacga gctggaaggc cagtaccgtg gcgaccagca
     2161 ctccgacctg agccggccac catggcaccc ggcgacgtac cgcaccgctc agccg
//
LOCUS       DATBFZ010000049         4277 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_1345140, whole genome shotgun sequence.
ACCESSION   DATBFZ010000049 DATBFZ010000000
VERSION     DATBFZ010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 4277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4277
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1345140"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..532
                     /locus_tag="VH419_01460"
     CDS             <1..532
                     /locus_tag="VH419_01460"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEY7042314.1"
                     /translation="RSLTDLAGEVAALSADDDVMSRLSPEVRDDWRSTAPALVSACAE
                     LDDLGPAPTLVHGDFHPWNVVRGADGVRIFDWSDAAVSHPFADLVSYVMRADDVSVRQ
                     RLFDRYLNSWRDHLPAQQLRRAGELALVVGSLYQVQTYRRIAAAVMPDGDLMSGDVSW
                     MKRSLDRLRHGLAAGG"
     gene            700..1308
                     /locus_tag="VH419_01465"
     CDS             700..1308
                     /locus_tag="VH419_01465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042315.1"
                     /translation="MFIQVIQGQVSDAGQVKAALERWSKDLQPGSSGWLGTTAGVTED
                     GRFIALARFESEEAAQRNSDRPEQGEWWAETAKLFTSDPTFSNSKDVMVDVNGDPDDA
                     TFVQVMQGRSSDPERAKELMSDDSVDWAAFRPEIIASVGCGHSGGAYTMAIYFTSEEA
                     AREGEKKEPPPELTAQMEEMDKLSVGTPEFYDLKDPWLISPS"
     gene            complement(1381..1905)
                     /locus_tag="VH419_01470"
     CDS             complement(1381..1905)
                     /locus_tag="VH419_01470"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF and ANTAR domain-containing protein"
                     /protein_id="HEY7042316.1"
                     /translation="MAELADSLQYELREGPCYAAVTYDRFVLVSDLTATTEFPRYGPL
                     AVELGIGSQAGIQLVHDGERAGLNLYSRASGAFDRSTLQFAELFATHAGALLGYADQV
                     EQLSEALHTRTDIGTAVGILMERYGIDRHQAFAFLARSSQNRNVKVRVLAQRVLDGTF
                     ESTPSEDSKSRQWP"
     gene            complement(2080..2769)
                     /locus_tag="VH419_01475"
     CDS             complement(2080..2769)
                     /locus_tag="VH419_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012923074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY7042317.1"
                     /translation="MALARFKKICVDAVDPIALGDFWAQVLGYRLQPDDRPEDEREAG
                     LVDADGSYRIWFNRVPEAKSVKHRVHLDVYAWTLADLEALGATVVLPAGEDRRWTVMQ
                     DPEGGEFCAFLRDVLPVRRLHGLVVDCEDAPAQARWWYDVLGGRHIDEGTWGTVTDLP
                     ELPDMTFDFVPVPEPKSAPNRIHWDVAVDSVDALLDRGATLLRAKGEGGIEWDVLADP
                     EGNEFCVFTSV"
     gene            2851..3681
                     /locus_tag="VH419_01480"
     CDS             2851..3681
                     /locus_tag="VH419_01480"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEY7042318.1"
                     /translation="MTQSLASYVDALPAEGNFSVWFGPLSGEPAYAHRADVQHYAAST
                     MKLALVMAAFRTAETGAIDLDAPVRIHDEFASAIDGSPFSLDRDEDSDEEPWQRIGTE
                     VALRWLCHRAIVRSSNLATNLVLEAVGTDAVAEVLAIVGAEHSVVTRGIEDSAAREAG
                     LQNLVTATDLARTLQALYVDGVASPANRDEILAVLAAQQINDAIPVRLPPGTRVAHKS
                     GWVEGISHDAGIVYPSPADGGEPFVFVMCTTSDLDEQAGLDLIAAGAAAAWEDRKVLS
                     "
     gene            3678..>4277
                     /locus_tag="VH419_01485"
     CDS             3678..>4277
                     /locus_tag="VH419_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEY7042319.1"
                     /translation="MSASDVIADVACTRLTAELHTPFVTALRSTSSVETVVVEITTTD
                     GAHGFGEAPQVWRVTGESLAGAEACIAGPLRDVLVGRGLDDFQDLLVECSAAVVANHG
                     AKAAVDVALHDLFARRLDVSLPRLLGSSRHRLPTDVTISAGDADRLGADARDRQAEGF
                     AVLKLKVGTDAETDVERVEAVRDAVGPEVRIRVDANQGWT"
BASE COUNT          707 a         1446 c         1457 g          667 t
ORIGIN      
        1 ccgttccctc accgacctcg cgggagaggt cgccgcgctc tccgccgacg acgacgtgat
       61 gtcgcggctg agcccggagg tccgcgacga ctggcgcagc accgcgccgg cgctggtgtc
      121 ggcctgcgcc gagctcgacg acctcgggcc ggcgcccact ttggtgcacg gcgacttcca
      181 tccctggaac gtcgtccgcg gcgccgacgg cgtccgcatc ttcgactggt cggacgccgc
      241 ggtctcacac ccgttcgccg acctggtctc ctacgtcatg cgagccgacg acgtgtcggt
      301 ccgacaacga ttgttcgacc gctacctgaa ctcgtggcgg gaccacctcc cggcacagca
      361 gttgaggcga gccggcgagc tcgcgctggt ggtgggctcg ctgtaccagg tgcagaccta
      421 ccgcaggatc gctgcggcag tgatgccgga cggcgacctg atgagcggtg acgtcagctg
      481 gatgaaacgc agtctggacc ggcttcggca cggtctcgct gccggcggtt gagcacgcgc
      541 atccggccca cggttccgaa ttgcggaaaa gtcccggctt ccactcggcg tgtcgacatg
      601 cgttttcccg cagttcgccg ggcgttacgc acaggctcga ccgtaggagg ggtcaggagt
      661 agcgtcgcaa gaggtccacg tcgaggaggg agtgacgtcg tgttcatcca ggtgatccag
      721 ggacaggtgt cggatgccgg gcaggtgaag gcagcgctcg agcgatggtc gaaggatctg
      781 caaccgggct cgtcgggatg gctcggtacg acggcgggtg tcaccgagga cggcaggttc
      841 atcgcactgg cccggttcga gtcggaggag gccgcccagc gcaacagcga ccgtcccgag
      901 cagggcgagt ggtgggccga gaccgccaag ctcttcacca gcgacccgac gttcagcaac
      961 agcaaggatg tcatggtcga cgtcaacggc gatcccgacg acgccacctt cgtgcaggtc
     1021 atgcagggcc gcagcagcga cccggagcgg gccaaggaac tcatgagcga cgactccgtc
     1081 gactgggccg cgttccgtcc agagatcatc gccagcgtcg gctgcgggca cagcggcggt
     1141 gcgtacacga tggcgatcta cttcaccagc gaggaagccg cccgcgaggg tgagaagaag
     1201 gagccgccgc cggagctgac ggcgcagatg gaggagatgg acaagctcag cgtcggcacg
     1261 ccggagttct acgacctcaa ggacccctgg ctcatctccc ccagctgact tgtcagatgc
     1321 aggaggacct gtagccccac gtacttctga caacttctgg gagcaggcgt acgtgagcgc
     1381 ctacggccac tgacgcgact tgctgtcttc actcggcgtg ctctcgaatg tgccgtcgag
     1441 gacacgttga gccaggacgc gcaccttgac gtttcggttc tgggagctcc gggccaggaa
     1501 ggcgaacgcc tggtgccggt cgatgccgta gcgctccatc aggatgccca ccgcggtgcc
     1561 gatgtcggtg cgcgtgtgga gcgcttcgct caactgctcg acctggtcgg catagccgag
     1621 caaggctccg gcgtgggtgg cgaagagttc ggcgaactgc agggtggagc ggtcgaaggc
     1681 tcccgatgcg cgggagtaca ggttgagccc ggcgcgttct ccgtcgtgga cgagttggat
     1741 cccagcctgg gagccgatgc cgagttcgac tgctaggggg ccgtagcgcg gaaactccgt
     1801 cgtggccgtc aagtcgctga cgagcacgaa ccggtcgtag gtgacggcgg cgtagcatgg
     1861 cccttcacgc agctcgtact gcaggctgtc ggcgagctcg gccaggtgat tccgctgacg
     1921 gaaacacgtc ttgtgttagg gacgggacgc gcttgttcaa gtggcgtcca tgccagcgta
     1981 cgcccgcaac cgacccgcgg gcgaccgagt gcactcgtat gccgcttcaa gcaccacgac
     2041 gttgagccgg atcagaccac cagtggtaga cgtgtcacat cagaccgacg tgaagacgca
     2101 gaactcgttg ccttcgggat cagccagcac gtcccactcg atgccgccct cgcccttggc
     2161 ccgcagcagc gtggcgcccc gatcgagcag cgcgtcgacc gagtcgactg ccacgtccca
     2221 gtggatgcgg ttcggcgcgg acttcggctc cggaaccggc acgaagtcga acgtcatgtc
     2281 cggcagctcc ggcaggtcgg tgacggtgcc ccacgtgcct tcgtcgatgt gccggccgcc
     2341 gaggacgtcg taccaccagc gcgcctgagc aggggcgtcc tcgcagtcga cgaccagccc
     2401 gtgcaaccgc ctgaccggga gcacatcgcg caggaacgcg cagaactcgc caccctcggg
     2461 gtcctgcatc accgtccagc gccggtcctc accggctggg agcacgaccg tcgcccccag
     2521 cgcctcgagg tcggccaacg tccaggcata cacgtccagg tggacgcgat gcttgacgga
     2581 cttggcctcc gggacgcggt tgaaccagat ccggtagctg ccgtcggcgt ccaccagacc
     2641 cgcctcgcgt tcgtcctcgg ggcggtcgtc cggctgcagt cggtagccga ggacctgcgc
     2701 ccagaagtca cccagcgcaa tcggatcgac ggcgtccaca cagatcttct tgaagcgggc
     2761 caacgccatg accggaaggc taaccgactt gtcagaagcc gatagacctc tggctccatc
     2821 agcttctgac aactcggtta gcctcggccc gtgacgcaat cgctcgcgtc gtacgtcgac
     2881 gcacttccgg cggaaggcaa cttctcggtc tggttcggcc cgctgtccgg cgaaccggcg
     2941 tacgcccacc gcgccgacgt ccagcactat gccgccagca cgatgaagct ggcactcgtg
     3001 atggccgcct tccggacagc cgaaaccggc gccatcgacc tggacgcacc ggtgcggatc
     3061 cacgacgagt tcgcttctgc catcgacggc tcaccgttct cactggaccg cgacgaggac
     3121 agcgacgagg agccctggca gcggatcggc accgaggtcg cgctgcgctg gctctgtcac
     3181 cgcgcgatcg tccggtcgag caacctcgcc accaacctgg tgctcgaagc cgtcggcacc
     3241 gatgcggtgg ctgaggtgct cgcgatcgtg ggcgccgaac acagcgtggt cactcgtggc
     3301 atcgaggact ccgcggcccg cgaggccggt ctgcagaacc tcgtcaccgc cactgatctg
     3361 gcgcgcacgc tccaggcgct gtacgtcgac ggcgtggcat cgccggcgaa ccgcgacgag
     3421 atcctcgcgg ttctcgccgc ccagcagatc aacgacgcca tcccggtgcg gctgccaccc
     3481 gggacccggg tcgcacacaa gtcgggatgg gtcgagggga tctcccacga cgccggcatc
     3541 gtgtatccga gcccggccga tggcggtgag ccgttcgtct tcgtgatgtg tacgacgtcc
     3601 gacctggacg agcaggcggg actggatctc atcgcggccg gagccgccgc cgcgtgggaa
     3661 gaccgtaagg tgctgtcgtg agcgcttcgg acgtgatcgc cgacgtggcc tgcacccggc
     3721 tgacggccga gctgcacaca ccgttcgtga ccgcactgcg ttcgacgtcg tccgtggaaa
     3781 cggtcgtcgt ggagatcacg acgacggacg gcgcacacgg cttcggcgag gcgccacagg
     3841 tgtggcgggt gacgggtgag tcgctcgccg gcgccgaggc ctgcatcgcc ggcccgctcc
     3901 gcgacgtact cgtgggtcgc ggtctcgacg acttccagga cctgctggtc gagtgcagtg
     3961 ccgccgtggt cgccaaccac ggggccaagg cggccgtcga cgtggcacta cacgacctgt
     4021 tcgccaggcg actcgacgtc tcgcttcccc ggctgctcgg ctcgtcccgg catcggctgc
     4081 cgaccgacgt cacgatctcc gccggcgacg cggaccggct cggcgccgac gcacgcgatc
     4141 gccaggccga ggggttcgcc gtgctcaagc tcaaggtcgg cacggacgcc gagaccgacg
     4201 tcgagcgcgt cgaggccgtc cgggacgctg tcgggcctga ggtccgcatc cgggtcgacg
     4261 ccaaccaggg atggacg
//
LOCUS       DATBFZ010000050         2315 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_2658683, whole genome shotgun sequence.
ACCESSION   DATBFZ010000050 DATBFZ010000000
VERSION     DATBFZ010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2658683"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..300)
                     /locus_tag="VH419_01490"
     CDS             complement(1..300)
                     /locus_tag="VH419_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042320.1"
                     /translation="MALARVVTFEGGTAEGLRAAVEEVNGQMRDRGGPPEGVKSIGMT
                     FLVDPDGGRAMFVGIFENEADLNDSTATLEAMSPPEGMGQRASVDVYEVGADVRM"
     gene            complement(363..1628)
                     /locus_tag="VH419_01495"
     CDS             complement(363..1628)
                     /locus_tag="VH419_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042321.1"
                     /translation="MVVGEPPLAQSSNVQLLGHIPGTAAGMNFKGHYAYVTGWAGVEV
                     LDIAQPEAPKVVGALTLPHFENEDVDLCGNTLIVVNDREVEDLGSVLYVVSIANPTTP
                     TIEAVLPLGLTGSGRGSGHIANFVKSDCTQLWVDGGDHVEVVDLTIPSAPRSLGKFVS
                     AASLSDAFNVSHDTEFDRPTNTIFNVGGGGAAAYKLTSNPLAPKLLGTTGTAGRNPSP
                     YNDFILHNSQRRGNTLLVTEEDYIDTDETPPGGCRGQGKFETWDLSRLSKGTIAPVDT
                     WETELNGMFTSGAVDSKAPVTVNCSSHWFDAKAGVAAVGWYEQGVRFLDYRTPGDIKQ
                     VGYYIPANGSTWAAYWSPTDKSGQIAYTADAYRGVDVVKIKDGGLTGKKVKAPVRDEW
                     LGSSTDSTIQPHPTFGFVCPVLRATVSVG"
     gene            1783..>2315
                     /locus_tag="VH419_01500"
     CDS             1783..>2315
                     /locus_tag="VH419_01500"
                     /inference="COORDINATES: protein motif:HMM:NF025691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4389 domain-containing protein"
                     /protein_id="HEY7042322.1"
                     /translation="MNTPEQYESSASGPSSPDYPVRFEVDYPEELNRWLPLVKWFLLI
                     PHYFALFFVIIAAFFVGVFAFFAVLITGRWPRGAFDFLVGTARWCYRVAAYFHLMTDR
                     YPPFSLADDPDYPARLEVDYPEQMNRWRPLVQWLLAIPYLIVASVLYYLTGIVTFIAF
                     FTVLFTKKIPRGLFELML"
BASE COUNT          336 a          801 c          766 g          412 t
ORIGIN      
        1 ctacatgcgc acgtctgcgc cgacctcgta gacgtcgacc gaggctcgct ggcccatccc
       61 ctccggcggg ctcatcgcct cgagcgtcgc ggtgctgtcg ttgaggtcag cctcgttctc
      121 gaagatcccg acgaacatgg cgcgaccgcc gtcggggtcg acgaggaagg tcatcccgat
      181 gctcttgacc ccctccggcg gaccgccgcg gtcccgcatc tgaccgttga cctcttcgac
      241 cgcagcacga aggccttcgg ccgtcccgcc ctcgaaggtg actactcgcg caagcgccac
      301 gactctcctc tcgatcgggt cctgcgcgga cactaaccga gcggtcagcg tcggcaaatg
      361 gcctagccga cggagacggt cgcccgcagg accgggcaga cgaagccgaa cgtggggtgc
      421 ggctggatcg tcgagtcggt cgagctgccg agccactcgt cgcgcaccgg cgccttgacc
      481 ttcttgccgg tcaggccgcc gtccttgatc ttcacgacgt cgaccccgcg gtaggcgtcg
      541 gccgtgtagg cgatctgacc ggacttgtcg gtcggcgacc agtaggccgc ccaggtcgag
      601 ccgttggcgg ggatgtagta gccgacctgc ttgatgtcgc ccggcgtgcg gtagtcgagg
      661 aagcgcacgc cctgctcgta ccagccgacg gcggccacgc ccgccttggc gtcgaaccag
      721 tgcgaggagc agttgacggt cacgggcgcc ttggagtcca ccgcgccgct cgtgaacatg
      781 ccgttcagct cggtctccca ggtgtcgacc ggcgcgatcg tccccttgct cagccgcgac
      841 aggtcccagg tctcgaactt gccctgcccg cggcacccgc ccggcggcgt ctcgtcggtg
      901 tcgatgtagt cctcctcggt gaccagcagc gtgttcccgc gtcgctgcga gttgtggagg
      961 atgaagtcgt tgtagggcga ggggttgcgg cccgcggtcc cggtcgtgcc gagcagcttg
     1021 ggagcgagcg ggttggaggt cagcttgtag gcggccgcgc caccgccgcc gacgttgaag
     1081 atcgtgttcg tcggccggtc gaactccgtg tcgtggctga cgttgaaggc gtcgctgagc
     1141 gaggcggccg acacgaactt gccgagcgac cgcggcgcgc tcgggatggt caggtcgacc
     1201 acctcgacgt gatcgccgcc gtcgacccac agctgcgtgc agtccgactt gacgaagttg
     1261 gcgatgtggc ccgagccgcg gccgctgccg gtcaggccga gcggcagcac ggcctcgatc
     1321 gtcggggtgg tcgggttggc gatgctgacc acgtagagga cggagccgag gtcctcgacc
     1381 tcacggtcgt tgacgacgat cagcgtgttg ccgcacaggt cgacgtcctc gttctcgaaa
     1441 tgcggcagcg tcagcgcgcc gacgaccttc ggcgcctccg gctgcgcgat gtcgagcacc
     1501 tccacgccgg cccagcccgt cacgtacgcg tagtggccct tgaagttcat cccggcggcg
     1561 gtgccgggga tgtgcccgag cagctggacg ttgctcgact gcgccagagg cggctcgccg
     1621 acaaccatct gagcactggc aggcagggcg gcgacgagtg tcgccgcgcc tgccagtacc
     1681 cccgtcgaga caaggcgcct gatcccccga gcgcgcacga taaacgcatg cgtaccccaa
     1741 acttcgaaag tccaatcctg cggcccattt acgcttgcgc gcgtgaacac tcccgagcag
     1801 tacgagagca gcgcctccgg cccgtcgtca cccgactacc cggttcgctt cgaggtcgac
     1861 taccccgagg agctgaaccg ctggctgcca ctggtcaagt ggttcctgct gatcccgcac
     1921 tacttcgccc tgttcttcgt gatcatcgcg gcgttcttcg tgggggtctt cgcgttcttc
     1981 gcggtgctga tcacgggccg ctggccgcga ggggcattcg acttcctcgt cggcacggcc
     2041 aggtggtgct accgcgtggc cgcctacttc cacctgatga cggaccggta cccgccgttc
     2101 agcctggcgg acgatcccga ctacccggcg cggctggagg tcgactaccc ggagcagatg
     2161 aaccgctggc gacccctggt ccagtggctg ctggcgatcc cgtatctgat cgtcgcctcg
     2221 gtcctctact acctcaccgg gatcgtcacc ttcatcgcgt tcttcacggt gctgttcacg
     2281 aagaagatcc cgcgcggcct gttcgagctg atgct
//
LOCUS       DATBFZ010000051         3064 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_363315, whole genome shotgun sequence.
ACCESSION   DATBFZ010000051 DATBFZ010000000
VERSION     DATBFZ010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 3064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3064
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363315"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            135..416
                     /locus_tag="VH419_01505"
     CDS             135..416
                     /locus_tag="VH419_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HEY7042323.1"
                     /translation="MITAIVFIKADVAQIPEVAQQIASLEGVSEVYSVTGDIDLIAIL
                     RVRNHDDVAALVPDLINKVPGVLSTETHIAFRTYSSHDLEAAFSLGVEE"
     gene            complement(540..1670)
                     /locus_tag="VH419_01510"
     CDS             complement(540..1670)
                     /locus_tag="VH419_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042324.1"
                     /translation="MSAYGHGRLRRGRSRSWLATGMTGALVGYAALAGAGTAAHAGTP
                     INDSARMMRADGRAHHSSEHIVWSQFVDLDFTGARIMVAASDGDSAHPLTYPAAGVVD
                     INPRISPNGRMVAFERDDSDGISHVMLVGIDGSGLHELDVCADPCIGANQPTWTPDGN
                     HLIFEKVIGPIDEGGNAQAAFLSKTDLTGDHEVRITPKRLDGVSEDANAQFAPAGYMV
                     VVRLNLARDRPALFRMRPDGTHACRLTPWALSADTQDVSPAISGPTKDLAVFETYGHN
                     EPPPGKVSAVATVKATCGGDHRIQYVTSPNREPVWHFNPAWSPDGKRLVFVRFKSVDG
                     DPIVHGDLVTSRWDGSDRRRIVQDPLFDFRPDWGPAPDPYAD"
     gene            1783..2838
                     /locus_tag="VH419_01515"
     CDS             1783..2838
                     /locus_tag="VH419_01515"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEY7042325.1"
                     /translation="MTATVEAVRGQLSDLVGRAAAGPEFGEAVANAIRTVLQFDAWCL
                     LGLNPGNGLRTFQFGHGGTGSEHTADMAANEAFQHDVNKYAELARAAVPAGCLSREHP
                     GAATSPRFQEIMRPQGIHSELRLVLRDGGRVWGAMSLFRDDPHRPLGDDDVDVMVALA
                     DPLGRAVRRFPTRPFATTPEPLAPGVVLLGPDNRMVAVSDQAWAWLEDLVPGGRDETW
                     LDDVTRVIYDAANVVRRRGGNGAGDGAGAAQSAVRTVSGRWLWVQGTALHDEPADVAV
                     VLQPAATRQLIGTLAVIHGLTRREVEVVERVTAGLATKQIAHDLAISPFTVNDHLKAI
                     YRKCSTAGREELLALIT"
BASE COUNT          476 a         1012 c         1043 g          533 t
ORIGIN      
        1 gctgggttgc gggttgttga gtgcgaggtt ggcgtgcgtt gccgtgagtc gtggttgtcg
       61 ggcctgactc gtctttccgg cgtcgggcgt tacgctcgtc ggcgtaccgt ccgacgccga
      121 ccgaggagtc gcccgtgatc accgcgatcg tgttcatcaa ggccgacgtg gcccagatcc
      181 cggaggtggc ccagcagatc gcatcgctcg agggtgtcag cgaggtctac tccgtgaccg
      241 gcgacatcga cctgatcgcg atcctgcggg tccgcaacca cgacgacgtc gcggcgctcg
      301 tgcccgacct catcaacaag gtgccgggcg tgctctcgac ggagacccac atcgcgttcc
      361 gtacctactc cagccacgac ctcgaggccg ccttctccct cggcgtcgag gagtagctcc
      421 attcgtcgaa ccgtgagttg tggttcgtcg aaccgtgagt tgtcgttcgt cgaaccgtga
      481 gttgtcgttc atcgagtcgt gacttgcggt ggctcgaagc gcgtcgtacg ccgtagtcgt
      541 cagtcggcgt acgggtccgg ggccggtccc cagtcagggc ggaagtcgaa caagggatcc
      601 tgcacgatcc gccggcggtc ggagccgtcc catcgactcg tcaccaggtc gccgtgcacg
      661 atcgggtcac cgtcgaccga cttgaaccgc acgaagacga gacgcttccc gtccggggac
      721 cacgccgggt tgaagtgcca gaccggctcc ctgttggggg aggtgacgta ctggatgcgg
      781 tgatcgccac cgcacgtcgc cttcacggtt gcgaccgccg agacctttcc gggaggcggc
      841 tcgttgtgac cgtaggtctc gaacaccgcc agatccttcg tcggtcccga aatcgccgga
      901 gacacgtcct gggtgtcggc gctgagcgcc cacggggtca ggcggcaggc gtgggtcccg
      961 tcgggcctca tccggaagag ggcaggccgg tcgcgagcca ggttcaagcg gaccacgacc
     1021 atgtagccgg cgggggcgaa ctgagcgttg gcgtcctcgg agacaccgtc gaggcgcttc
     1081 ggagtaatgc gaacctcgtg gtcgcccgtc aggtctgtct tcgacagaaa cgccgcctgg
     1141 gcattgccgc cttcgtcgat cggcccgatc accttctcga agatcaggtg gttgccgtcc
     1201 ggggtccacg tcggctggtt cgcaccgata cacggatcgg cgcagacatc gagctcgtgc
     1261 agtccgctcc cgtcgatccc cacgagcatc acgtgactga tgccgtcgct gtcgtcgcgc
     1321 tcgaaggcga ccatgcgccc gttcggcgag atcctcgggt tgatgtcgac gacgcccgcg
     1381 gcagggtacg tcagcgggtg cgcgctgtct ccgtcggagg cggcaaccat gatccgggca
     1441 ccggtgaagt cgaggtcgac gaactgcgac cacacgatgt gttcactcga atggtgagcc
     1501 cggccgtcgg ctcgcatcat gcgcgcggag tcgttgatcg gcgtccctgc gtgcgctgcg
     1561 gtgccggcgc cggccagtgc ggcgtaccca acgagcgctc ctgtcatccc ggtggcgagc
     1621 caactcctgc tgcgaccgcg ccgcagcctt ccgtgaccgt atgcactcat cgcatgcccc
     1681 tccgtcgcct cgacgcgtgt cgcggttgct gtgagtgtcc gacgacatcg tgagttcgga
     1741 aatccctact tccagggatg tgggctctgg cacgatcgag agatgaccgc cacggtcgag
     1801 gcggtgcgtg gacaactttc cgatctggtc ggacgcgctg cggcggggcc tgagttcggc
     1861 gaggcggtgg ccaacgccat acggacggtc ctgcagttcg acgcgtggtg cctcctcggg
     1921 ctcaaccccg gcaacggact gcgtacgttc cagttcggcc acgggggcac cggcagcgag
     1981 cacaccgctg acatggcagc gaacgaggcg ttccaacacg acgtcaacaa gtacgccgag
     2041 ctcgcccgcg ccgccgtgcc ggccggctgc ctgtcccgtg agcatcctgg ggctgcaacg
     2101 agtccgcgtt ttcaggagat catgcgtccc cagggcatcc actccgagct ccggcttgtg
     2161 ctgcgggacg gcgggcgcgt ctggggagcg atgtcgttgt tccgggacga cccgcatcgc
     2221 ccgctcggcg acgacgacgt cgacgtcatg gtggcgctgg ccgacccgct cggtcgggcg
     2281 gttcggcggt ttccgactcg cccgttcgcc acgacgcctg aaccgctggc gcccggcgtc
     2341 gtactgctgg gtcctgacaa ccggatggtg gcggtctccg accaggcgtg ggcctggttg
     2401 gaggacctcg tgccaggtgg ccgcgacgag acctggctgg acgacgtgac gagggtcatc
     2461 tacgacgccg cgaacgtcgt acgccggcgg ggtggcaacg gtgctggtga cggcgctggt
     2521 gccgcgcagt cagccgtccg tacggtcagc ggtcggtggc tgtgggtgca ggggaccgcg
     2581 ctgcacgacg agccggccga cgtggctgtg gtgctccaac cggctgcgac gcggcagctc
     2641 atcgggaccc tggccgtcat ccacgggctc acgcgacggg aggtggaggt ggtcgagcgt
     2701 gtcacggcgg gccttgccac gaagcagatc gcccatgacc tcgccatctc gccgttcacc
     2761 gtcaacgacc acctcaaagc gatctatcgc aagtgcagca ctgccggacg cgaagagctg
     2821 ctcgccctga tcacctgact gagccttcat gtcgagcggt aggtcgatct gggaaccaga
     2881 agtcacggtt cgctgaacca caactcacgg ttcgctgaac cacaacccac ggctcaacga
     2941 accacaaccc acgactcaac gaaccacaac tcacggttca acggaggcgg cggcgtggag
     3001 ccaggtggcg agcttggcct tggcagcgcc ggagtcgacg gcctcagcag ccttctgcat
     3061 cccc
//
LOCUS       DATBFZ010000052         2814 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_1346892, whole genome shotgun sequence.
ACCESSION   DATBFZ010000052 DATBFZ010000000
VERSION     DATBFZ010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2814
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1346892"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..968
                     /locus_tag="VH419_01520"
     CDS             <1..968
                     /locus_tag="VH419_01520"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEY7042326.1"
                     /translation="TYRERTRTATLQRQYGTTTRYGRRRRSLLLLGVLLVVLLAATAF
                     AAARVLLHDNDKDRTYLSTDGWPARGQGAYLLGDGQAAVSPHEQPVPIASLAKVMTAY
                     LVLKHDPLSTGDDGRRFVVEQRDVQDTERRRREDQSVVDVRAGEQLTERDALMAILLP
                     SANNVAVLVARQVDGSVASFVAEMNRTAHALGMSHTTYTDPSGFDEHTVSTALDQLRL
                     AQRVADNDTLAAMMATRSYSMPVADRVTNTNTLLGEDGFVGMKTGSDDAAGGCFMFRS
                     WRHTERGNVPLTGVVLGQQGHNLITAGLYAAKQLVDQVAPTAATP"
     gene            1063..1497
                     /locus_tag="VH419_01525"
     CDS             1063..1497
                     /locus_tag="VH419_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014174167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY7042327.1"
                     /translation="MATEFQVTFDSADPGAHAAFWAEAVHYIEQPPPSGFDSWDAALD
                     SWGVPAEHRNDRAALVDPAGAGPRLFFQKVPEPKTAKNRVHLDLRAAAGVAPEGRADA
                     LEAECTRLVALGARRVQRLEADGINGVCIVMQDPEGNEFCLD"
     gene            complement(1516..1839)
                     /locus_tag="VH419_01530"
     CDS             complement(1516..1839)
                     /locus_tag="VH419_01530"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEY7042328.1"
                     /translation="MAKFIYLYRGPATPLSDLTPEQGAERTAAFGAWMEKVGTALVDV
                     GSPFGASASVRDDGTEVEAGDLIGYTIVAADDLAAAKALTDGLPFLSNGDGTCAVEIF
                     ELLPM"
     gene            2069..2674
                     /locus_tag="VH419_01535"
     CDS             2069..2674
                     /locus_tag="VH419_01535"
                     /inference="COORDINATES: protein motif:HMM:NF012859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RICIN domain-containing protein"
                     /protein_id="HEY7042329.1"
                     /translation="MSTDLTNQPTYAVTEDASEPAAKPRRRRVTRVLAAATTMALAPA
                     VLLGLAAGPASASTYSATNVYLASDSSASTLFVDVSGASRSWGAQVIQWSLNGGANQK
                     WDIQNLADGNKEFVNANSGQCLTTDGIAGDGVYQFPCVGAKNQEWTTNLVVGNFHNYT
                     IKSASSGLNLDVSGNSSNQGAVIDTWYDNGGGNQHFFGYQG"
BASE COUNT          498 a          987 c          922 g          407 t
ORIGIN      
        1 tgacctaccg cgaacgaact cgaacggcaa cgctgcaacg tcagtacggg acgacaacgc
       61 ggtacggccg ccgccggcgc agccttctcc tgctcggcgt gctactcgtc gtactccttg
      121 ccgccacggc gtttgcggct gcccgcgtac tgctccacga caacgacaag gaccgcacct
      181 acctgtcgac cgacgggtgg ccggcccgcg gacagggggc gtacctgctc ggcgacggcc
      241 aggccgcggt gagcccgcac gagcagccgg ttccgatcgc cagcctggcg aaggtgatga
      301 ccgcctacct cgtgctcaag cacgatccgc tgagcacggg cgacgacggt cggcgattcg
      361 tcgtcgagca gcgcgatgtc caggacaccg agagaaggcg ccgcgaggat caatcggtcg
      421 tcgatgtccg cgccggcgag cagctcaccg agcgtgacgc cctcatggcg atcctgttgc
      481 cgtccgcgaa caacgtcgcg gtgctcgtcg cccgccaggt cgacggcagc gtcgcctcgt
      541 tcgtcgcgga gatgaatcgc acggcccacg cgctggggat gtcgcacacg acctacaccg
      601 acccgagtgg tttcgacgaa cacaccgtct cgactgcgct cgaccaattg cggctggcac
      661 agcgggtcgc ggacaacgac acactcgccg cgatgatggc gacgcgcagt tattcgatgc
      721 cggtcgcgga cagagtcacg aacaccaaca ccctgctcgg cgaggacgga ttcgtcggca
      781 tgaagaccgg ctcagacgac gcagccggcg gctgcttcat gttccggtca tggcggcaca
      841 ccgagcgcgg caacgtcccg ctgaccggcg tagtgctcgg gcagcaaggg cacaacctga
      901 tcaccgcggg gctgtatgcg gccaagcagt tggtcgacca ggtggcaccg accgcggcga
      961 ccccctgatc gtggacccga tggtgaacac gtcgtgtcgg ggccaatccc gggtggttcc
     1021 cgaggcggac acgttgtcgg tgggttgcgc gaaggtcgat gcatggcgac cgagttccaa
     1081 gtgacgttcg acagtgctga tccgggtgcg cacgctgcct tctgggccga ggcggtgcac
     1141 tacatcgagc agccaccacc gtccggcttc gactcgtggg acgccgccct cgattcctgg
     1201 ggcgtgccgg ccgagcaccg caacgaccgc gcggcgctgg tcgaccccgc aggtgcaggg
     1261 cctcggctgt tcttccagaa ggtgccggag ccgaagacgg cgaagaaccg cgtacacctc
     1321 gacctgcgcg ccgccgccgg tgtggcgccg gagggccggg cggacgcgct cgaggcggag
     1381 tgcacccgcc tggtcgcgct cggcgcgcgt cgggtgcagc ggttggaggc cgacggcatc
     1441 aatggcgtct gcatcgtcat gcaggacccg gaaggcaacg agttctgcct cgactgaggg
     1501 atggtccggc ggacgctaca tcggcagcag ttcgaagatc tccaccgcac acgtcccgtc
     1561 accgttggac aggaacggca ggccgtcggt gagcgccttc gccgcagcaa gatcgtcggc
     1621 cgcgacgatc gtgtagccga tcaggtctcc ggcctccact tccgtaccgt cgtcgcgaac
     1681 ggacgcgctc gccccgaacg ggctgccgac gtcgacgagc gccgtaccga ccttctccat
     1741 ccaggcgccg aacgcggccg tccgctcagc gccttgctcc ggcgtgaggt cggacaacgg
     1801 cgttgccggg ccacggtaga ggtagatgaa cttcgccatg tcgagcgtcc cttcaacaac
     1861 gcggaccaat gaccttcgac cgttaagacg aacagcgccg gcgcggacgg acaactgaat
     1921 gcctcagtca acctcgcgcg tcggccgctg agggggtgcg ggcaccccct cgcgaagacg
     1981 gtggccggcc aggtgggacc ggtgatcgga ttcggcgagg ctttctcgga cgcggtcacc
     2041 cggaccgcca ccacagaagg aatcgcgcat gagcaccgac ctgaccaacc agcccaccta
     2101 cgccgtcacc gaggacgcct ccgagcccgc cgcgaagccc cgcaggcgtc gcgtcacccg
     2161 agtgctcgcc gccgccacca cgatggccct cgcgccggcg gttctgctcg gactggccgc
     2221 cggccctgct tccgcgagca cgtactccgc gaccaacgtc tacctggcct cggacagcag
     2281 cgcgtcgacc ctcttcgtgg acgtgtccgg cgcatcccgt tcgtggggcg cgcaggtcat
     2341 ccagtggagc ctcaacggcg gcgccaacca gaaatgggac atccagaacc tggccgacgg
     2401 caacaaggaa ttcgtcaacg ccaacagcgg gcaatgcctg accaccgacg gcatcgccgg
     2461 agacggcgtg taccagttcc cctgcgtcgg cgccaagaac caggaatgga ccaccaacct
     2521 cgtcgttggc aacttccaca actacacgat aaaaagcgca agcagtgggc tgaacctcga
     2581 cgtgtccggc aacagcagca accaaggcgc ggtcatcgac acgtggtacg acaacggcgg
     2641 cggcaaccag cacttcttcg gctaccaggg ctgaccgtcc agcaccagca ccggtcgagt
     2701 tcgttcgcca tccttgtccg cccgccatac gctcggacag cacacccggt ttggcggtcg
     2761 catggctggt cgatcgagcc gcccgttgtg gcggcgcgcg gcgatcggct gctg
//
LOCUS       DATBFZ010000053         3133 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_3316304, whole genome shotgun sequence.
ACCESSION   DATBFZ010000053 DATBFZ010000000
VERSION     DATBFZ010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 3133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3133
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3316304"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="VH419_01540"
     CDS             complement(<1..229)
                     /locus_tag="VH419_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042330.1"
                     /translation="MTRTVRIALCDPGGEVLGALPPFELETPWWQDTGPAVVEARRRY
                     GVELTILRIVDTEDRWPPHGGRVTYLAEVDGP"
     gene            250..1974
                     /locus_tag="VH419_01545"
     CDS             250..1974
                     /locus_tag="VH419_01545"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HEY7042331.1"
                     /translation="MPTAAEIKAQLTGPGGMFEVVTETVRGRPMQVYKERMRALRQIC
                     AVAQARGDQTFIVYGDREISFREFIAGSNSVSAGLQALGIGPGDRVAVLSQNNPEWCM
                     TFWGTVNVDAILVGLNGWWKTDEVIYGLQDSGAKVLVADGKRFERIADSLDECPDLEH
                     VFLVEASPSDFPVADKTGKLHPFSDLLGAATDTMPDVPIDEDDYAVIFYTSGTTGRPK
                     GAISTHRSMIANLQNTMYGSVAGAMLGGGAMPDASAGGGQTVALFTSPLFHVSGCHST
                     LVVGLLAGIKLIMVEGRFDPEKALSLIEEHKVALWATVPTMVWRVCEDPHRHDYDTST
                     VVSVAFGGSPSADELQRKVHETFPNVRSTTNAYGLTESSSVATAISGQDAVDKPTSVG
                     PAVPTLEIVIRDEHGAPVETGVAGEVTMYGPLITAGYWNKPDATAETVRDGWLYTGDV
                     GYLDADGYLYITDRKKDMIIRGGENIYCVEIENRLVENPRIADAAVIGVPHPELGEEV
                     KAVVQVEPGETITEDEVRQWVGETLANFKVPTYVEVRDDKLPRNASGKLLKNVLRGTG
                     DVSFDETM"
     gene            complement(2045..3007)
                     /locus_tag="VH419_01550"
     CDS             complement(2045..3007)
                     /locus_tag="VH419_01550"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HEY7042332.1"
                     /translation="MDINLAIEAVNVIASRQAGAFTSQQATAAGVTRRVRRRLVTQGV
                     WSVRGRGLLVVAGSPGGWLQDLWLAHLAAGPASVICGRSAARLHGLRGQWGEAVEIVV
                     PHDGAHDAAHGRVRRTRYLPEHHVVHIDGLPVTSVARTIFDRAGDPGNPVMFRRDDLV
                     EIHERQLRRVVRDAMVRRGLTMPGLIRVTAALARRGRAGSAVMRSLVRHLGAGYRPTE
                     SELEDAFLDLIDAEGLERPENQVDVVGERGWLGRVDFMYRSRRLIIEVDGPDHDDEER
                     ILEDKRRDDGLAEAGWSVIRIHWTTLLFDADAVAAEIRRGLSSP"
BASE COUNT          531 a         1036 c         1111 g          455 t
ORIGIN      
        1 cgggcccgtc gacctccgcc agataggtga cgcgtccgcc gtgcggaggc catcggtcct
       61 cggtatcgac gatccgcagg atcgtgagct cgacgccata gcggcgccga gcctccacga
      121 cggccgggcc cgtgtcctgc caccacggcg tctcgagctc gaagggagga agcgcgccga
      181 ggacctcgcc accggggtcg cagagcgcga tgcggaccgt cctcgtcacg cttgacagaa
      241 tgcccggcca tgccgaccgc tgcggagata aaggctcagc tcactgggcc tggagggatg
      301 ttcgaggtcg tcaccgaaac ggttcgggga cggccgatgc aggtctacaa ggagcggatg
      361 cgcgcgctcc ggcagatctg cgcggtggcg caggcccggg gcgaccagac gttcatcgtc
      421 tacggcgacc gcgagatctc gttccgcgag ttcatcgccg gctcgaactc ggtgagcgcg
      481 gggctgcagg cgttgggcat cgggccgggc gaccgggtgg cggtgctgtc gcagaacaac
      541 cccgagtggt gcatgacctt ctgggggacg gtcaacgtcg acgcaatcct cgtggggctg
      601 aacgggtggt ggaagaccga cgaggtgatc tacgggctgc aggactcggg ggccaaggtc
      661 ctcgtggccg acggcaagcg gttcgagcgc atcgccgact cactcgacga gtgccccgac
      721 ctcgagcacg tgttcctggt ggaggcgtcg ccgtcggact tcccggtggc cgacaagacc
      781 gggaagctgc acccgttctc ggacctgttg ggtgccgcca ccgacaccat gccggacgtg
      841 ccgatcgacg aggacgacta cgcggtcatc ttctacacca gcggcacgac ggggcggccg
      901 aagggcgcga tcagcaccca ccgcagcatg atcgccaacc tccagaacac gatgtacggc
      961 agcgtggccg gggcgatgct ggggggcgga gcgatgcccg acgcctcggc gggcgggggc
     1021 cagacggtcg ccctgttcac gtcgccgctg ttccacgtgt cgggctgtca ctcgacgctc
     1081 gtggtggggc tgctcgccgg gatcaagctg atcatggtcg agggtcggtt cgatcccgaa
     1141 aaggcgctgt cgctcatcga ggagcacaag gtggcgctgt gggccacggt gccgacgatg
     1201 gtgtggcgcg tgtgcgagga cccgcaccgg cacgactacg acacgtcgac ggtggtgtcg
     1261 gtggcgttcg gcgggtcgcc ctcggccgac gagctgcagc gcaaggtgca cgagacgttc
     1321 cccaacgtgc gcagcaccac caacgcgtac ggcctcaccg agtcgtcgtc ggtggcgacg
     1381 gcgatctcag gtcaggacgc ggtggacaag ccgacgtcgg tggggccggc cgtgccgacg
     1441 ctggagatcg tgatccgcga cgagcacggg gctccggtcg agacgggtgt cgcgggcgag
     1501 gtcaccatgt acggcccgct gatcacggcc gggtactgga acaagcccga cgcgaccgcc
     1561 gagacggtgc gggacggctg gctgtacacg ggcgatgtcg gctacctcga cgccgacggg
     1621 tacctctaca tcaccgaccg caagaaggac atgatcatcc gcggcggcga gaacatctac
     1681 tgcgtcgaga tcgagaaccg gctggtggag aacccgcgca tcgccgacgc cgcggtgatc
     1741 ggcgtgccgc atcccgagct gggcgaggag gtcaaggccg tcgtccaggt cgagccgggc
     1801 gagaccatca ccgaggacga ggtgcgccag tgggtcggcg agacgctcgc caacttcaag
     1861 gtccccacgt acgtcgaggt ccgggacgac aagctccccc gcaacgccag cggcaagctg
     1921 ctgaagaacg tgttgcgcgg caccggcgac gtcagcttcg acgaaaccat gtaacccctt
     1981 gcctacgaga ttctgaggag ggttgtggcc gacagatggc cacaaacctc cccagatccg
     2041 ggggctacgg cgacgacagg ccacgacgaa tctcggcggc gacggcgtcg gcgtcgaaga
     2101 gcagcgtggt ccagtggatg cggatgacac tccagccggc ctcggcgaga ccgtcgtcgc
     2161 ggcgcttgtc ctcgaggatc cgctcttcgt cgtcgtggtc gggaccgtcg acctcgatga
     2221 tgagacggcg gctccgatac atgaagtcca ctcgtccgag ccagccgcgc tcgccgacca
     2281 cgtcgacctg gttctccggc cgttcgagac cctccgcatc gatcaggtcg aggaacgcgt
     2341 cctcgagctc gctctccgtc ggccgatagc ccgcgccgag atgccgcacg agcgatcgca
     2401 tgaccgccga tccggcccga ccccgccggg cgagcgccgc ggtcacgcga atgagacccg
     2461 gcatcgtgag gcctcgccgc accattgcat cgcggacaac gcgccgcagc tgtcgttcgt
     2521 ggatctcgac gaggtcgtcg cgtctgaaca tgacggggtt acccgggtcg ccggcgcgat
     2581 cgaagatcgt ccgagccacg gatgtcacgg gaagcccgtc gatgtgaacg acgtggtgct
     2641 ctggaagata tctcgtccgg cgcactcggc cgtgggcggc gtcatgtgcg ccgtcgtgcg
     2701 gaacgacgat ctccaccgcc tcgccccact gaccccgcag gccgtggagt cgggcggccg
     2761 atcggccgca gatgaccgag gccggaccag cggccagatg cgccagccag aggtcctgca
     2821 gccatccacc cggcgagccc gcgacgacga gcagtccacg gccgcgcacc gaccagacac
     2881 cctgcgtcac cagccgacgt cgcacgcgcc gggtgacgcc ggcggcggta gcctgctgcg
     2941 acgtgaaggc gccggcttgg cgcgaggcga tcacattgac cgcttcgatg gcgaggttga
     3001 tgtccacgtc aggttgtact gcggacgtgt gacaccccgt tctgcggagg attgtggcca
     3061 actggtggcc acaatcctcc tcagaacggg aaagggctag gtgacggcgc cgccgctggc
     3121 gtcttcgacg cgc
//
LOCUS       DATBFZ010000054         2670 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_5269309, whole genome shotgun sequence.
ACCESSION   DATBFZ010000054 DATBFZ010000000
VERSION     DATBFZ010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2670
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5269309"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1041)
                     /gene="dinB"
                     /locus_tag="VH419_01555"
     CDS             complement(<1..1041)
                     /gene="dinB"
                     /locus_tag="VH419_01555"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEY7042333.1"
                     /translation="MTNDRGCTIIHVDMDAFYAAVAIRDRPELQDQAVIAGGGDRGVV
                     LSATYPAREYGVRSAMPMTRARRLCPHAVVVMPDYDEVSQVSSAVMELFRSITAKVET
                     LSMDEAFLDVSGSLRRFSSPTRIGEYLRATIYDEQRITCSVGIAETTQLAKLASQRAK
                     PDGLVVVPREEVVSFLHPLGVEELWGVGEKTAEQLHQFGLRTVADLAYTPVAVLQRAL
                     GAGTGARLHAMAWGDDDREVVARRGPDEPEHSIGNNETFYRDTDDDAVILRELLRLSN
                     KVTSRMRSARVAGRTITLSVRFSDFTTITRSRTLRDHTNLTPEIYATAADLFLGLGLE
                     RARIRRVGVRVEG"
     gene            complement(1147..1938)
                     /locus_tag="VH419_01560"
     CDS             complement(1147..1938)
                     /locus_tag="VH419_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012920492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY7042334.1"
                     /translation="MVERRSAIRAALVWDALFPVLQARHEAVTSAHDNRDKRPLEVVD
                     LGGGTGGLAVRIAELGPDVVQVLVVDPSPDALASLERRAAEADVADAVRGVLGDAATL
                     LDLVTAGTVDVVVCHEVLEVVDVPAQAVEAAAAVLAPGGVLSLVAAQRSAAVFARAVA
                     GHVTDARTLLTDPDGRWGSTDPMPRRFTRDELTGLLEDAGLTVAEVRGVRVFTDHLSS
                     ANVEPERSGHGDGTAEELHMLEAEVATHPDFLAIATQLHLLATKR"
     gene            complement(2105..>2670)
                     /locus_tag="VH419_01565"
     CDS             complement(2105..>2670)
                     /locus_tag="VH419_01565"
                     /inference="COORDINATES: protein motif:HMM:NF013499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HEY7042335.1"
                     /translation="TQTPPIRSGRRVVFLTLDDGSGPVDATFFEDAQGPYAATLFHSW
                     LLVVRGELRRTGPKGVSLRATGCWELTAMYEAWKHGGVEAARDLMAAAPLGFTGEQAD
                     AAVKGAAESRSTRPVMAQPPRFDDPAEERSGEPATKAGGMGRRRVLVHASGFKQSPYA
                     DIRPAGEDTKHGVPRKLWHSSPGSSGP"
BASE COUNT          430 a          943 c          906 g          391 t
ORIGIN      
        1 accctcgacc cggacgccca cccggcggat ccgggcacgt tcgagcccga gtcccaagaa
       61 caggtcggcc gccgtcgcat agatctccgg agtcaggttg gtgtggtcac gcaacgtgcg
      121 ggaccgggtg atcgtggtga agtcggagaa ccgcacgctc agcgtgatcg tccggccggc
      181 aacccgcgcc gaccgcatcc gggatgtcac cttgttcgac agtcggagca gctcacggag
      241 gatgacggcg tcgtcgtcgg tgtcgcggta gaacgtctcg ttgttgccga tgctgtgctc
      301 gggttcgtcc ggaccacgac gcgccaccac ctcgcggtcg tcgtcgcccc acgccatcgc
      361 atgcagccgg gcgccggtgc ctgcccccaa ggcccgctgc aacaccgcaa cgggcgtgta
      421 tgccaggtcg gcaaccgtcc gcaggccgaa ctggtgaagc tgctcggccg tcttctcccc
      481 caccccccac agctcttcga cgccgagcgg gtgcaggaac gacaccacct cctctcgcgg
      541 tacgacgacc agaccgtcag gcttggcacg ttgtgaggcc agcttggcca actgagtggt
      601 ctcggcgatc ccgaccgaac aggtgatgcg ctgctcgtcg tagatcgtgg cgcgcaggta
      661 ctccccgatc cgggtgggtg aggagaatcg ccgtagtgac cccgagacgt cgaggaacgc
      721 ctcgtccatc gacaacgtct ccaccttcgc agtgatgctg cggaacagct ccatcaccgc
      781 cgacgagacc tggctgacct cgtcgtagtc gggcatcacc accaccgcat gagggcacag
      841 ccgacgggcc cgggtcatcg gcatggcgga ccggacgccg tactcacgag cggggtaggt
      901 ggccgacagc accacaccac gatcgccacc tccggcgatg accgcctggt cctgcagctc
      961 gggacgatca cggatcgcca cagctgcgta gaacgcgtcc atgtcgacat ggatgatcgt
     1021 gcagcctcgg tcgttcgtca ccgcgtaccc cctgccgggc tgaccgcgaa atgcgggaaa
     1081 acggaggtcg acacgtcgat gccggcatga cctttcccgc aattcgcagg agccacccag
     1141 ggagggtcag cgcttggtgg cgagcaggtg cagctgggtg gcgatggcca ggaagtccgg
     1201 atgcgtggcc acctccgcct ccaacatgtg cagctcctcg gccgtcccgt ccccatgccc
     1261 cgaccgctcc ggctcgacgt tcgcgctgct gaggtggtcg gtgaacacgc gtacgccgcg
     1321 aacctcggcc accgtgagcc cggcatcttc gagcagtccg gtgagctcgt cgcgggtgaa
     1381 ccggcgtggc atcgggtcgg tgctccccca gcgcccgtcc ggatcggtga gcagggtgcg
     1441 cgcatcggtg acgtgcccgg cgacggcgcg agcgaagacg gcggccgaac gctgcgcggc
     1501 caccagactg aggaccccgc ccggtgcgag cacagcggct gctgcctcca ccgcctgcgc
     1561 ggggacgtcg acgacctcga ggacttcgtg gcagacgacc acgtcgacgg tcccggcggt
     1621 gaccaggtcg agaagggtgg ccgcgtcacc gagaacgccg cgaacggcat cggcgacgtc
     1681 cgcctcggcg gcgcgtcgtt cgagcgaggc caacgcgtcg ggactggggt cgaccaccag
     1741 gacctggacg acatcgggac cgagctcggc gatccggacg gccaagccgc cggtgccacc
     1801 tccgagatcg acgacctcca acggccgctt gtccctgttg tcatgggcac tcgtgacggc
     1861 ctcgtggcga gcctggagca ccgggaacaa cgcgtcccag accagggcgg cccggatcgc
     1921 actgcggcgt tcgaccacga gcagtctcct gatggctcac cgatgaccac ctctcagcca
     1981 ctgccgcccg ggcgagcggc gctcagcggg atgcatcgca aggcagagga ggaagccgat
     2041 acttgagcga agcgtatcgg cgacgacgat aacgccgcga ggcgcccgct gagcgtcgct
     2101 ggggtcatgg gccggagctt ccgggggacg agtgccacag tttcctgggg acaccgtgct
     2161 tggtgtcctc cccggccgga cggatgtcgg cgtacggcga ctgcttgaac ccgctggcgt
     2221 gcacgagcac ccgccgccgg cccatgccac cggccttcgt cgcgggctcg ccggaccgtt
     2281 cctcggccgg gtcgtcgaag cgtggcggct gcgccatcac cggccgggtc gaccggctct
     2341 cggccgctcc cttcaccgca gcgtccgcct gctcgccggt gaagccgagt ggtgcggcag
     2401 ccatcaggtc gcgggcagcc tcgacacctc cgtgcttcca ggcttcgtac atcgcggtca
     2461 gctcccaaca gccggtggcg cgcagtgaga cgcccttggg gccggtacgc cgcagctcgc
     2521 ctcgaaccac cagcagccag gagtggaaca aggtggcggc gtacggacct tgcgcgtcct
     2581 cgaagaacgt cgcgtcgacc ggcccgctcc cgtcgtcgag ggtcaggaac accacccgtc
     2641 gaccggaccg gatcggtggc gtctgggtgg
//
LOCUS       DATBFZ010000055        16814 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_1022831, whole genome shotgun sequence.
ACCESSION   DATBFZ010000055 DATBFZ010000000
VERSION     DATBFZ010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 16814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16814
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1022831"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..421
                     /locus_tag="VH419_01570"
     rRNA            <1..421
                     /locus_tag="VH419_01570"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            535..651
                     /gene="rrf"
                     /locus_tag="VH419_01575"
     rRNA            535..651
                     /gene="rrf"
                     /locus_tag="VH419_01575"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            761..1570
                     /locus_tag="VH419_01580"
     CDS             761..1570
                     /locus_tag="VH419_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY7042336.1"
                     /translation="MPAGDTSPRRPRRDQPGRDRSKDRTRSTAGRWRRDELPAVERTA
                     VQAAYDGPPIPDDITGKELDRYVVNQLKSLPEKLAARVARHLVAAARLIDTDPETAYQ
                     HTLAARARASRVAGVREASGEAAYAAGRFKDALTEFKAARRMNHSPIYLPMMADCERG
                     LGRPERALTLAKDPAVAELDEAGQIEMKIVESGARRDLGDAKAAIRTLDGKELRSRSR
                     APWVARLRYAYAEALLADGQADRAREWFERAAGVDTGGHTDAADRVAALDN"
     gene            1775..2119
                     /locus_tag="VH419_01585"
     CDS             1775..2119
                     /locus_tag="VH419_01585"
                     /inference="COORDINATES: protein motif:HMM:NF012651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEY7042337.1"
                     /translation="MTQEDNEVVLRGRVAAPVDERQLPSGDTIMTARLIVDRDETARA
                     RSSQRVDTIDCVAWLRRVQRTMRTWQAGEQVEISGAIRRRFFRGVSGPVSRVEVEIRT
                     ARRLTPRRPDNA"
     gene            complement(2091..3392)
                     /locus_tag="VH419_01590"
     CDS             complement(2091..3392)
                     /locus_tag="VH419_01590"
                     /inference="COORDINATES: protein motif:HMM:NF018004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HEY7042338.1"
                     /translation="MPCRGRGRRWLRCSVAPEWQSDGERERDPGAPQPRLRLRPFPAT
                     RFDPSVVGDIGEVTSPPYDVMDRAMIDDLLNEHPRNIVRLILPQMVSDPVRSGDPYSR
                     AARLLTRWRANGVLRTDREAALYVYEYGDDTRRVCGVVGALDLRKRTASVVLPHEGVI
                     PRVVAERLAMMASARANLEPILLVYNGDGSTEPVLGRARQRQPLIDVRAGDQTFHRVW
                     SITDRTELAALRRALEPHQALIADGHHRYATYLQLRKRHRSIGDGKGPWDRGLALLID
                     HAEYPLQLGPIHRSISELSLADLVAPPGFELSPAQRFSGLPAPPDEQGCFVVTDGSAV
                     RRLRLTDVRESAVSDAELLHARVLPGWGGGEDQVGYHHTVDQTLRAAHQDGGVAVLLH
                     PTSVAEVMEVARAGKMMPRKSTSFGPKPRMGLLMRCLDDEA"
     gene            3399..4457
                     /locus_tag="VH419_01595"
     CDS             3399..4457
                     /locus_tag="VH419_01595"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HEY7042339.1"
                     /translation="MTPVRGVLLGSSDEPLATAYDCAMVDLDGVVYVGEHAVEGVPEL
                     LSKAREHGMAIAFVTNNAARTPEAVAAHLVALGVQAEAADVVTSAQAAAREVAARVEP
                     GARVLVVGGEGLRAALAEHGLVAVSSAQDRPHAVVQGFHPAISWALLAEGGYAVRAGA
                     VWIASNLDLTIPTQHGIAPGNGTLVNAVAIAAGRRPDVVAGKPFPPLFDETVRRIAST
                     RPLVVGDRLDTDIEGAVTCGADSLLVMTGVTGVAELCRADPRRRPTYVSWTMSGLLQA
                     HRAPQPTDGAWSLGGWTARVNEGGRLEVVDRATSDDDGLPVVAAACWTWLDAQDDEDP
                     YRRLDLSGLDGVWSGPHR"
     gene            4485..4694
                     /locus_tag="VH419_01600"
     CDS             4485..4694
                     /locus_tag="VH419_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042340.1"
                     /translation="MTDQRATGDDRVDAVTARLRELDDRDVHDHADVYEAIHRDLAAV
                     LDDAAALPVTPDASDSTAEDGPTVP"
     gene            4714..5520
                     /locus_tag="VH419_01605"
     CDS             4714..5520
                     /locus_tag="VH419_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020386558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEY7042341.1"
                     /translation="MTRRLRLDAELVRRQLARSRDHAAELIAAGRVRVGGAVATKAAT
                     SVTTDVALVVRDDPSSPDYVSRGGHKLVGALEAFAAGGLVVQGRRALDAGASTGGFTD
                     VLLRAGVDAVVAVDVGYGQLAWTLQQDPRVTVLDRTNVRDLRTAMLPFRPELVVGDLS
                     FISLGKVLPALRDVSHPDADFVLMVKPQFEVGKQRLGSRGVVRDPALRVEAILDVAEA
                     AAELGLGVVDVCASPLPGPSGNVEYFVWFRRGAGPVDPQHVQTVVAEGPR"
     gene            5534..6472
                     /locus_tag="VH419_01610"
     CDS             5534..6472
                     /locus_tag="VH419_01610"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019674 - NAD metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEY7042342.1"
                     /translation="MTEPNAQGRTVLLVTHTGRSEAVEVATAVAKRLLASDIAVRVLV
                     DEAADLPITGVAAVTAGADAADGCELAVVIGGDGTILRAAELTRFTETPLLGINLGHV
                     GFMAEAEWDDLDTTVEAIIGGTLSGPDVVEERMTLDVRVLLDGVEIGSGWALNEASVE
                     KASRQRMLEAVVEIDGRPLSRWGCDGVVFATPTGSTAYAFSAGGPVVWPEVEALLLVP
                     ISAHALFARPMVTSPDSTLAVELLAESDGGVLWCDGRRPMELPTGARIEVRRGGHPVR
                     LARLHEAPFTDRLVAKFDLPVSGWRGGAERRRGRQR"
     gene            6472..8166
                     /gene="recN"
                     /locus_tag="VH419_01615"
     CDS             6472..8166
                     /gene="recN"
                     /locus_tag="VH419_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012922985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEY7042343.1"
                     /translation="MLEELAIRSVGVIDEAVLELGPGFTVVTGETGAGKTMVVTALGL
                     LLGARADVGAVRGGAGKARVDGRVRIDPGSPAWRRADDIGADLDDETLILERTVSAEG
                     RSRANVGGAAVPVGTLADLTADLVTVHGQSDQQRLLQPAVQRETLDAFGGGSHAELVD
                     RFRTSYDGLTTIRAELEDIVQRRRERAQEADALRFGLGEVEAASPEPGEDVSLAAEEG
                     RLAHVEGLRQAADVARFALSGDETDVDGRDALSLVAAARKAVEAERHHDDRLADLAEA
                     LASASYALADVAADLSAYATSLDVDPARLGWVQDRRATLTALTRKYGDTIDDVLAWSG
                     RAASRLLELEDDDARLEALRGEEAAAAEAMAAAGTELSAARRALADALAGRVSTELAE
                     LAMPQAEFTVDVRPGETPTRSGYDEVTFCFAAHAGAPARPLQRGASGGELSRVMLALE
                     VCLVGTQPVPTMIFDEVDAGVGGKAAVEVGRRLARLAKAVQVIVVTHLPQVAAFADQH
                     YVVVKTDDGRVTTAGVTALDEAGRRSELSRMLAGLEDSESALAHADELLELRHSLD"
     gene            8314..9492
                     /gene="steA"
                     /locus_tag="VH419_01620"
     CDS             8314..9492
                     /gene="steA"
                     /locus_tag="VH419_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020577470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cytokinetic ring protein SteA"
                     /protein_id="HEY7042344.1"
                     /translation="MKLSLRKPRSTHDVGVSGTARLDRRTRTMAKRLRPGDIAVIDHV
                     DIDRHAALALIDADVAAVVNIAPSISGRYPNLGPELIVDAGIVLVDDVGSAVFQAVND
                     GDLVRIEGDTIYRGDTAVGVGVRHDPASVAAAMEAAKDGMASQLEAFSANAVEHLRRE
                     RALLLDGEGVPSLSTRVDGRDVLVVLRTFDYAADLRSLRTYIRENSPVLIGVDAGADA
                     LLEAGHRPDIVVTGAEDISDRALRCGAEVVAHAGRDGRIPGAERLDRLATPHTTFCGG
                     GTSEDAAILLAHAHGAGLIVMVGSHATLVELLDKGRSAMASSFLTRATVGSSLVDAKA
                     VARLYRHRVRTWVVLLVLLVGIAAVVAAIATTPVGQDWWDQVTAWYADASDWVAGLTS
                     "
     gene            9489..10433
                     /locus_tag="VH419_01625"
     CDS             9489..10433
                     /locus_tag="VH419_01625"
                     /inference="COORDINATES: protein motif:HMM:NF022824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper transporter"
                     /protein_id="HEY7042345.1"
                     /translation="MTALRRWVFPILVALLAFAIGIALGGGPLQTDEGGETDAGLARD
                     KQALQDDVASLRSDRTFDDGLSQALSARLLDKRLEGRSVTLVVLPGIDERSVAATEQD
                     VKLAGADVPVVVRLDDGLLNPGKKTYVDSVATNSLKGASDLASLAGASTYERAGALVA
                     RAYAGKGNNADVDDEAARIDSELQGAELVAVDGTPIRRGTFVVVLAPGEHGQDSFTRA
                     QMVIAGDLVTALVHGSDAVVVGSSPTSSQSGGLIDVLTADQDLASTRLSTINVADSPA
                     GRIALVYALAAAASGAPGHYGVDGDEAVLPPGLAPNDD"
     gene            10617..12311
                     /locus_tag="VH419_01630"
     CDS             10617..12311
                     /locus_tag="VH419_01630"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEY7042346.1"
                     /translation="MPTKHLFVTGGVASSLGKGLTASSLGRLLKSRGLRVTMQKLDPY
                     LNVDPGTMNPFQHGEVFVTNDGAETDLDIGHYERFLDVDLNQQANVTTGQVYSTVIAK
                     ERRGDYLGDTVQVIPHITNEIKQRIRAMAGPDVDVVITEIGGTVGDIESLPFLEAARQ
                     VRHDIGRDNCFFLHVSLVPYIGPSGELKTKPTQHSVAALRSIGIAPDAVVCRADREIP
                     ANIKRKISLMCDVDDEAVVAAVDAPSIYDIPKVLHAEGLDAYVVRRLNLPFRDVDWRR
                     WDELLQRVHHPDEEVTVALVGKYIDLPDAYLSVAEALRAGGFEHSARINLRWVASDEC
                     QTPEGASRLLGDVDAVCVPGGFGIRGIEGKVGALTYAREHRIPTLGLCLGLQCMVIEF
                     ARNVAHLVEAGSTEFDIDTAQPVIATMEEQLDIVDGAGDMGGTMRLGLYPADLQPGSI
                     AAEAYGSTRVEERHRHRYEVNNKFRGDLEDAGLVFSGLSPDHELIEFVELPREVHPYY
                     VSTQAHPELRSRPTRPHPLFSGLVAAALERQRELRLPVDETALRSTAYWPAPQPIE"
     gene            12326..12928
                     /locus_tag="VH419_01635"
     CDS             12326..12928
                     /locus_tag="VH419_01635"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008359438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY7042347.1"
                     /translation="MKAEGRPADTPESWPVLSTTTESEGAIVSVFSEEIEAPDGGRFV
                     RQVVRHPGAVAVVAVDSEDRVLVLSQYRHPVRQRLVELPAGLLDVDGEDPESAAVREL
                     AEEAQLSARHWSVLVDVLTSPGIHDEAVRIFLAEELSEIDLPAGFEAEHEEADMSRHW
                     VRLDELVTAILDGEVKDSLTVSGSLALWARRHGYGSGKQL"
     gene            12990..14105
                     /gene="ald"
                     /locus_tag="VH419_01640"
     CDS             12990..14105
                     /gene="ald"
                     /locus_tag="VH419_01640"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020577439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEY7042348.1"
                     /translation="MKVGVPKEVKNHEYRVAITPAGVHELSRRGHEVYVQASAGIGSS
                     INDDEFVAAGAKTLPTADDVWETGDLILKVKEPIAEEYHRMRDGQTLFTYLHLAASRD
                     CTDALIKQGVTAIAYETVQTPDGALPLLAPMSEVAGRLAPQCGAYHLMRQGGGRGVLM
                     GGVSGVYAAKVVVIGAGVSGMNAAAIALGMQAEVLLLDRNIERLRQADRIYQGHLQTV
                     ASNSYEIERAVIDADLVIGAVLVPGAKAPMLISNELVSRMKPGSVLVDISIDQGGCFE
                     DSRPTTHAEPTYRVHNSVFYCVANMPGAVPHTSTYALTNVTLPYAVSLADKGWKQALL
                     DDHSLALGLNTHQGEVTYEPVATAHGLDHRSLDSVLS"
     gene            14137..15072
                     /gene="xerD"
                     /locus_tag="VH419_01645"
     CDS             14137..15072
                     /gene="xerD"
                     /locus_tag="VH419_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEY7042349.1"
                     /translation="MVAEPSNLRGAIRTYLDHLAVERGLAANTVASYRRDLRRYDGFL
                     AGQGVRALSDVTEPMVAEFLMRLREGDAAHQPLSAGSAARTLVAVRGFQKFAVREGLA
                     DHDPAATVKPPTPAKRLPKALPLSDVEAILAAAGAPGTTLALRDRALLEVLYGTGARI
                     SEAVGLDIDDVDLAEGVVRLLGKGGKQRLVPIGSYARDALDAYLVRGRPMLADGGRGT
                     PAVFLNARGGRLSRQSAWTVLGKAADRAGVTADVSPHTMRHSFATHLLDGGADVRVVQ
                     ELLGHASVTTTQIYTLVTVDKLREVYAASHPRALG"
     gene            15318..16199
                     /locus_tag="VH419_01650"
     CDS             15318..16199
                     /locus_tag="VH419_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017936236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY7042350.1"
                     /translation="MGQFTTVAESPPVLEMGPTGRPLPVFPEPPPLIEHGGATVIAMC
                     NQKGGVGKTTTVINLGASLAEYGRKVLLVDFDPQASLTVGLGINPNELGTTIYHLMLE
                     REVPLSDVVVRTGVEGVDLIPSNIDFAGAEMQLINEVAREQVLARALAPFVAAYDVIL
                     IDCQPSLGLLTVNALAAADGVIVPLECEYFALRGVALLKGTIDKVQDRLNPKLQIIGL
                     LGTMYDGRTLHSREVLQTLVDGWGDAVFHTVIRRTVKFSEATVAGEPLTAYATASNGA
                     DSYRQLAREVLTRCHDA"
     gene            16184..16513
                     /locus_tag="VH419_01655"
     CDS             16184..16513
                     /locus_tag="VH419_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012922685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042351.1"
                     /translation="MSRRVTLPGADELFGSTEKPSRAEPPSPAAGDGQSATGPSGRIR
                     HDEKITVYVTADELLDLEHTRLSLRRDHGLAVDRGRLVREAIHLALEDAATNGSDSAL
                     VRRLTAS"
     gene            16510..>16814
                     /locus_tag="VH419_01660"
     CDS             16510..>16814
                     /locus_tag="VH419_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEY7042352.1"
                     /translation="MTAPLLDRESESGFAVHLDNFEGPFDLLLQLIAKHKLDITEVAL
                     SKVTDEFIVHTKAMGADWGLDQTTEFLVVAATLLDLKIVRLLPSAELEDDEDLALLEA
                     "
BASE COUNT         2625 a         5683 c         5934 g         2572 t
ORIGIN      
        1 tcgacgggat ggtttggcac ctcgatgtcg gctcgtcgca tcctggggct ggagtaggtc
       61 ccaagggttg ggctgttcgc ccattaaagc ggcacgcgag ctgggtttag aacgtcgtga
      121 gacagttcgg tccctatccg ctgcgcgcgc aggagacttg agaagggctg cccctagtac
      181 gagaggaccg gggtggacga acctctggtg tgccagttgt tctgccaaga gcacggctgg
      241 ttggctacgt tcgggagtga taaccgctga aagcatctaa gcgggaagca cgcttcaaga
      301 tgaggtttct caccgggtga accgggtaag gcccccagca gaacactggg ttgataggcc
      361 ggaggtcgaa ggcagcgatg cacggagccg accggtacta ataggccgag ggcttgtctt
      421 ctttctcatc ttcattgttc tttgttcgcg tccactgtgc ggttcccgag atacggtcgg
      481 gaccggtcga ggttcagcca gcctggaccc ccgccggcta tatctccata gagtttcggc
      541 gaccatggcg aaggggaaac acccggctcc attccgaacc cggaagttaa gcccttcagc
      601 gccgatggta ctgcacgcgg gagcgtgtgg gagagtagga cgtcgccgga cattcttgtg
      661 aagggggtcg ctttttcgaa gcggccccct tcgtcatcat caccaccatc atgatgagtt
      721 cagggtcttc ctgaattcag cgcgcatcga ggagtccgcc atgcccgccg gcgacacgag
      781 cccccgccgt cctcgcaggg atcagccggg ccgggaccgc agcaaggacc gcacccgcag
      841 tactgcggga cgctggcgtc gcgacgagct cccggcggtc gaacggactg cggtccaggc
      901 agcgtacgac ggtccgccta ttccggacga catcaccggc aaggagctcg acaggtacgt
      961 cgtcaaccag ctcaagtcgc tgccggagaa gcttgcggcc cgggtcgcgc gccatctggt
     1021 ggccgccgcc cggttgatcg acaccgaccc ggagacggcg taccagcaca cactggcagc
     1081 acgggcacgc gcgtcccgcg tcgcgggcgt gcgcgaagca agcggcgagg ccgcctacgc
     1141 cgccggccgc ttcaaggatg ccctgacaga gttcaaggcc gcccggcgaa tgaaccacag
     1201 cccgatctac ctgccgatga tggccgactg tgagcgtggc ctcggccgtc ccgagcgggc
     1261 gctcacgctg gcgaaggatc ccgctgtcgc cgagctcgac gaggctggac agatcgagat
     1321 gaagatcgtg gagtccggcg ctcgacgcga cctcggcgac gcgaaggcgg cgatccgaac
     1381 cctcgacggc aaagagctgc ggagcaggtc tcgtgccccg tgggtggctc ggctgcgcta
     1441 cgcctacgcc gaagccttgc tggccgacgg acaggccgac cgggcacggg agtggttcga
     1501 acgcgccgcg ggtgtcgaca ccggcggcca caccgatgcc gccgaccggg tcgccgcgct
     1561 cgacaactga cgatccgcgc aacgccgagg tggcaagttt cgttgggttt tgtcggcgtg
     1621 tcgcgccctg agtgccacct cgtcactctt ctgagcgagc ctcacaacat gcttccccag
     1681 ttcgccgatc ggccggcatc ggtccacaac attccactgc gcggcgttcc gcccatcccc
     1741 actcagcatg atggcccgag aagggagcca tccgatgacc caagaggaca acgaggtcgt
     1801 cctgcgcgga cgcgtcgcgg caccggtcga cgaacgacag ttgccgagcg gcgacacgat
     1861 catgaccgcg cgcctcatcg tggaccggga cgagaccgct cgtgcccgct ccagccagcg
     1921 cgtcgacacg atcgactgtg tcgcgtggct gcgccgggtg cagcgcacga tgcgtacctg
     1981 gcaggcaggt gaacaggtgg agatctccgg tgcgatccgg cgcaggttct tccgcggagt
     2041 gagcggcccg gtgagccggg tcgaggtcga gatccgtacc gcccgccggc tcacgcctcg
     2101 tcgtccagac aacgcatgag gagccccatg cgcggcttgg gcccgaacga cgtggacttg
     2161 cgcggcatca tcttcccggc acgcgccacc tccatgacct cggccacgga ggtggggtgg
     2221 aggagaaccg cgacaccgcc gtcctggtgc gccgcccgca acgtctggtc gacggtgtgg
     2281 tgatacccga cctggtcctc accacctccc cagcccggga gcacccgcgc gtgcaacagc
     2341 tccgcgtcgc tgacggcgct ctcgcggacg tcggtgagcc gcagtcggcg taccgcggaa
     2401 ccgtcggtga cgacaaagca gccctgttcg tcaggcggtg ccggcaggcc ggagaaccgc
     2461 tgcgccggag acagctcgaa ccccggcggc gccacgaggt cggccaacga aagctcggag
     2521 atgctccggt ggatcgggcc gagctggagc ggatactccg cgtggtcgat cagcagcgcc
     2581 aggccgcgat cccacggacc cttgccgtca ccgatggagc ggtgccgctt gcgcagctgc
     2641 aggtacgtcg cgtagcggtg atgcccgtcg gcgatcagcg cctggtgcgg ttccagggcc
     2701 cggcgaagtg cagccaactc cgtccgatcc gtgatcgacc acacccgatg gaatgtctgg
     2761 tcaccggcgc ggacgtcgat gagcggctgc cgctgccgcg ctcggccaag caccggctcg
     2821 gtggaaccgt caccgttgta gacgagcagg atgggctcga ggttcgcacg tgcgctcgcc
     2881 atcatcgcca gccgttcagc gacgacccgg gggatcacgc cctcatgggg gagcaccacg
     2941 ctggcggtgc gtttgcgcag gtccaaggcg ccgacgacgc cacacacccg gcgggtgtcg
     3001 tcgccgtact cgtagacgta cagcgcggcc tcgcggtcgg tgcgcagtac gccgttcgcg
     3061 cgccaccggg tgagcagccg ggccgcccgg gagtacggat ctccggaacg gaccgggtcg
     3121 ctcaccatct gcggcaggat cagccggacg atgttgcgag gatgctcgtt cagcaggtcg
     3181 tcgatcatgg cgcggtccat cacgtcgtac ggtggcgacg tgacctcacc gatgtccccg
     3241 accacggacg ggtcgaaccg agtcgccgga aatggtcgta gccgcaggcg cggctgcgga
     3301 gcaccggggt cccgctcccg ctcgccgtcc gattgccact ctggggccac gctgcatcgt
     3361 agccagcggc gcccccgtcc tcggcacggc atagcacgat gacacccgtc cgcggcgtcc
     3421 tgctggggag cagtgacgag ccgctcgcga ccgcctatga ctgcgcgatg gtggacctcg
     3481 acggagtggt gtacgtcggc gagcacgccg tcgagggagt tcctgagctg ctctcgaagg
     3541 cacgggagca tgggatggcg atcgccttcg tcacgaacaa cgctgcccgc acgcccgagg
     3601 cggtcgctgc gcacctggtg gcgctcggtg tgcaggcgga ggctgcggac gtcgtcacct
     3661 ccgcccaggc ggctgcccga gaggtggcag cccgggtcga gcccggcgcc cgggtcctgg
     3721 tcgtcggtgg ggagggtctg cgcgcagcgc tcgccgagca cggtctcgtc gcggtctcgt
     3781 cggcgcagga ccgaccgcat gcggtggtgc aggggttcca tccggcgatc agctgggcgc
     3841 tgctggccga aggcgggtac gccgtccggg ccggcgcggt ctggatcgcc tcgaaccttg
     3901 acctgacgat cccgacccag catggcatcg cgcccggcaa cgggacgctc gtgaacgcgg
     3961 tcgcgatcgc ggccggccgc cggccggacg tggtcgccgg caagccgttc ccaccgcttt
     4021 tcgacgagac ggtccgacgc atcgcctcga cgcggccgct ggtcgtgggc gaccggctgg
     4081 acaccgacat cgagggtgcg gtgacgtgcg gggctgattc gctgctggtg atgactgggg
     4141 tcaccggagt tgccgagctg tgcagagccg acccgaggcg ccgaccgaca tacgtctctt
     4201 ggacgatgag tggcctgctg caggcccatc gcgccccaca gcccacggac ggtgcgtggt
     4261 cgctgggggg ctggactgca cgggtgaatg agggcggtcg cctggaggtc gtcgaccggg
     4321 cgacatcgga cgacgacggg ctcccggtcg tggcggcggc atgctggacg tggctcgacg
     4381 cgcaggacga cgaagacccg tatcgacgcc tcgacctgtc aggcttggac ggtgtttgga
     4441 gcggtcccca ccggtaggtt gtccgcgtgc ccgaaggagt ccccatgaca gaccagcgcg
     4501 ccaccggtga cgaccgggtc gacgccgtga cggcccggct acgtgagctg gacgaccgcg
     4561 acgtccacga ccacgccgac gtctacgagg cgattcaccg tgacctggca gcagtgctcg
     4621 acgacgcagc agcgctgccg gtcacaccgg acgcctcgga ctcaacggcg gaggacggcc
     4681 cgacggtccc gtgagcacgc agcggcggcc ctgatgacgc gccggcttcg gctcgacgcc
     4741 gagctcgtac gccgccagct ggcccgcagc cgcgaccacg ccgccgagct gatcgcggcc
     4801 ggccgggtcc gggtgggcgg cgcggtggcg acgaaggcag ccacgagcgt cacgaccgac
     4861 gtcgcgcttg tcgtacggga cgacccgtcc agcccggact acgtgtcgcg aggcgggcac
     4921 aagctcgttg gcgctctcga agcgttcgct gcgggcgggc ttgtggtgca gggccggagg
     4981 gcgctggacg ccggtgcgtc gaccggcggc ttcaccgacg tcctgctgcg cgccggggtg
     5041 gacgccgtcg tcgcggtcga cgtcggctac ggccagctcg cctggacgct tcagcaggat
     5101 cctcgcgtca ccgtcctcga ccgcaccaac gtcagagatc tgcgcacagc aatgctgccg
     5161 ttccggccgg agttggtggt cggtgatctg tcgttcatct cgttggggaa ggtgctaccg
     5221 gcgttgcgcg acgtgagcca ccccgacgcc gacttcgtgc tgatggtcaa gccgcagttc
     5281 gaagtgggca agcagcgcct cggcagtcga ggcgtcgtcc gcgaccctgc cctgcgcgtc
     5341 gaggcaatcc tcgacgtcgc cgaagcagcg gccgagctgg gcttgggggt ggtagacgtc
     5401 tgcgcgagcc cgcttcccgg accgtcggga aacgtcgagt acttcgtctg gttccggcgc
     5461 ggcgccggcc cggtcgaccc acagcatgtg cagaccgtcg tcgcggaagg tccgaggtga
     5521 gaggctgtct ggcgtgaccg aaccgaacgc ccaaggccgg acggtgctgc tcgtcacgca
     5581 taccggacgc agcgaggccg tcgaggtggc gaccgccgtg gcgaagcgat tgctggcctc
     5641 cgacatcgct gtccgtgtgc tcgtcgacga ggcggcggac ctgccgatca ccggcgtggc
     5701 tgcggtcacc gccggcgccg atgcggcgga tgggtgtgag ctcgccgtcg tgatcggagg
     5761 cgacggcacg atcctgcggg cggccgagct cacccggttc accgagaccc cgttgctggg
     5821 catcaatctc ggccacgtcg gcttcatggc agaagccgag tgggatgacc ttgacaccac
     5881 cgtcgaggcg atcatcggcg gcaccctgtc aggcccggat gtggtcgagg agcggatgac
     5941 gctcgacgtc cgggtgctgc tcgacggcgt cgagatcggc tcgggctggg cactgaacga
     6001 agcgagtgtc gagaaggcat cgcggcagcg gatgctcgag gccgtcgtgg agatcgacgg
     6061 ccgcccgctc tcccggtggg gctgcgacgg cgtcgtcttc gccacgccga cggggtcgac
     6121 cgcgtacgcg ttcagcgccg gcggacccgt cgtctggccg gaggtggagg ccctgttgct
     6181 ggtcccgatc agcgcccacg cactgttcgc aaggccgatg gtgacgtcgc cggactcgac
     6241 cctcgcggtc gagctgctcg ccgagtccga cggcggcgtt ctctggtgtg acgggcgtcg
     6301 tccgatggag ctgccgacag gtgcccggat cgaggtacgc cgtgggggcc atccggtgcg
     6361 actggcgcgc ctgcacgagg cgccgttcac cgaccgtctg gtggcgaagt tcgacctgcc
     6421 cgtgtcgggg tggcgcgggg gggcggagcg ccgccgcggg agacaacgct gatgctcgag
     6481 gagctcgcca tccggtcggt cggcgtgatc gacgaggccg tcctcgagct cggcccgggt
     6541 ttcaccgtcg tcaccggtga gaccggtgcc gggaagacga tggtggtgac ggcgctcggc
     6601 ttgctgctcg gcgcccgtgc agacgtcggg gcggtgcgcg gcggtgcagg aaaggctcgt
     6661 gtcgacggcc gggtccggat cgacccgggg tcaccagcct ggcggcgagc cgacgacatc
     6721 ggcgccgacc tggacgacga gacgctgatc ctggaaagga cggtctcggc ggaggggcgt
     6781 tcgcgcgcca acgtgggtgg ggctgcggta ccggtgggca cgctcgccga tctcacggcc
     6841 gacctggtca ccgtgcacgg ccagtccgac cagcagcggc tgctgcaacc ggcggtgcag
     6901 cgggagacgc tggacgcgtt cggcggcggc tcgcacgccg agctggtcga ccgattccgg
     6961 accagctacg acgggttgac cacgatccgc gccgagctcg aggacatcgt gcagcgaaga
     7021 cgcgaacgcg cccaggaggc cgacgccttg cgcttcgggc tcggcgaggt ggaagccgcg
     7081 agcccggagc cgggcgagga cgtgtcgttg gccgccgagg aggggcggct cgcgcacgtg
     7141 gagggtctgc ggcaggcggc cgacgtcgcc cggtttgccc tgagcggtga cgagaccgac
     7201 gtcgacggac gtgacgcact cagtctggtt gctgccgccc ggaaggccgt cgaagcagaa
     7261 cgtcaccacg acgacaggct cgctgacctc gcggaggcgc tggcctcggc gtcgtacgcg
     7321 ctcgccgacg tcgcggcgga cctctcggcg tacgccacat cgctcgatgt cgacccggcc
     7381 cggctcgggt gggtgcagga ccggcgtgcc acgctcaccg cattgacccg caagtacggc
     7441 gacaccatcg acgacgtgct ggcatggtcg ggccgggccg ccagccggct cctggagctc
     7501 gaggacgacg acgccaggct cgaggcacta cgaggcgagg aggccgcggc ggccgaggct
     7561 atggcggcgg caggtacgga actgtcggcg gcccgccgtg ccctggccga cgcgctggca
     7621 ggtcgcgtga gcaccgaact ggccgagctg gccatgccgc aggccgagtt cacggtcgac
     7681 gtcagacccg gggagacccc gacgcggagc ggctacgacg aggtcacgtt ctgcttcgct
     7741 gcgcatgcag gcgcgcccgc ccggccgctg caacgcggcg cctccggcgg cgagctgtcg
     7801 cgggtgatgc tggcgctcga ggtgtgcctg gtcgggacgc agccggtgcc gacgatgatc
     7861 ttcgacgagg tcgatgccgg ggtcggcggc aaggcggccg tcgaggtcgg gcgccgcctc
     7921 gcccggctcg ccaaagcggt ccaggtcatc gtggtgaccc atctcccgca ggtggcggcg
     7981 tttgccgacc agcactatgt cgtggtcaag actgacgacg gcagggtcac gacggcgggt
     8041 gtcaccgcgc tcgacgaggc gggccgccgc agcgagctgt cccggatgct ggccgggctg
     8101 gaggactccg agtcggcgct ggcgcatgcc gacgagctgc tggagctccg acatagcctt
     8161 gactgagcgt tgaccgagcg ttgactgagc gttgactgag cgttgactga gcgtgtcgca
     8221 ctcaagggct gacccgggcc gacaaggtcg accgtcgggt tggcgcgtcg cttcctgggt
     8281 tcccttgggc tcgtttggca ggattggcgc acgatgaagc tctccctacg caagccacgt
     8341 tccacgcacg acgtcggggt ctccggaacg gcacgcctcg accggcgtac ccgcaccatg
     8401 gccaaacggc ttcgtcccgg cgacatcgcc gtgatcgatc acgtcgacat cgaccgacat
     8461 gccgcgctcg ccctgatcga tgccgatgtc gcggcggtcg tcaacatcgc tccgtcgatc
     8521 tccgggcgtt acccgaacct cggacccgag ctgatcgtcg acgccggcat cgtgctcgtg
     8581 gacgacgtcg ggtcggccgt cttccaggcg gtcaacgacg gcgacctggt gcgcatcgag
     8641 ggtgacacga tctatcgggg tgacacggcg gtgggcgtcg gcgtacgcca cgacccggcc
     8701 tccgtcgccg ccgcgatgga agcggcgaag gacggcatgg cgagccagct cgaggcattc
     8761 agcgcgaacg cggtcgagca tctgcggcgg gagcgcgcac tgctgctgga cggcgaaggc
     8821 gttccgtcgt tgtcgacccg ggtcgacggt cgcgatgtgc tggtcgtgct acgcacgttc
     8881 gactacgccg ccgatctgcg gtcgctcaga acgtacatcc gtgagaactc acccgtactc
     8941 atcggcgtcg acgcgggtgc cgatgcgttg ctcgaggcag gccatcgtcc ggacatcgtg
     9001 gtgaccggcg ccgaggacat ctccgaccgc gccctgcgct gtggcgccga agtcgtcgca
     9061 catgcaggac gcgacggccg gatccccggt gccgagcgcc tcgatcggtt ggcgaccccg
     9121 cacacgacgt tctgcggcgg cggcacttcc gaggacgccg cgattctgct cgcccatgcg
     9181 cacggggccg gactgatcgt gatggtcggg tcgcacgcga cgcttgtcga gctgctcgac
     9241 aaggggcggt cggcgatggc gagctcgttc ctcacgcggg cgacggtggg ttcgagcctg
     9301 gtcgacgcga aggccgtcgc ccgcctctac cggcatcggg tgcggacctg ggtggtgctg
     9361 ctggtactgc tcgtcggcat cgcggcggtc gtcgcggcga tcgccaccac gcccgtgggt
     9421 caggactggt gggaccaggt cacggcctgg tacgccgacg cgtccgactg ggtcgcagga
     9481 ctgacgtcgt gaccgcactc cggcgatggg tctttcccat cctggtggcg ctgctggcct
     9541 tcgcgatcgg gatcgccctg ggcggcggac ccctgcagac cgacgaaggt ggcgagaccg
     9601 atgccgggct ggcccgcgac aagcaggcgc tccaggacga cgtggcgtcg ctgcgttcgg
     9661 accgcacgtt cgacgacggc ctcagccagg cactgtcggc gcggctcctc gacaagcgac
     9721 tcgaagggcg cagcgtgacg ctggtcgtgc tgcccggcat cgacgagcgc tcggtcgccg
     9781 ccaccgagca ggacgtgaag ctggccggtg ccgacgtgcc cgtcgtcgtc cggctcgacg
     9841 acggactgct gaaccccggc aagaagacgt atgtcgacag cgtggcgacc aactccctca
     9901 aaggcgcgtc cgacctcgcg agccttgccg gcgccagcac ctacgagcgc gccggcgccc
     9961 tggtcgcccg cgcgtacgcc gggaagggca acaacgccga cgtcgatgac gaagcggcca
    10021 ggatcgactc cgagctgcag ggcgcggagc tcgtcgccgt cgacggtacg ccgatcaggc
    10081 gtggcacgtt cgtcgtcgtc ctcgcacccg gtgagcacgg ccaggacagc ttcacgcggg
    10141 cccagatggt catcgcgggc gacctggtca cggccctggt gcacggaagc gacgcggtcg
    10201 tcgtcgggtc cagcccgacg tcgtcgcagt ccggcgggct gatcgacgta ctcaccgccg
    10261 atcaagatct ggcttcgacg cggctctcca cgatcaacgt cgcggactct ccggctggtc
    10321 ggatcgccct cgtctatgcg ttggcggccg ccgcatcggg tgcccccggc cactacggcg
    10381 tggacggtga cgaggccgtg ttgccacccg gtctggcgcc caacgacgac tgaccagcac
    10441 gtctgccgcg ctcgtcggcg tgtccgttga ttgagtctgt cgaaatcgag gacctctgaa
    10501 gcgttcgggg tttcgacaag ctcaaccgcc ggaagggttg ctaggctgga atcccgtgga
    10561 agggcggtga ctcaccgccg tacgtcgacc cgcgggagac ctcgttggcc gtttcgatgc
    10621 cgaccaagca ccttttcgtc accggcggcg tggcgtcctc gttgggcaaa ggcctcacgg
    10681 catcgtcgct cggccggctg ctgaagtcgc gcggactgcg ggtcacgatg caaaaactcg
    10741 acccgtacct caacgtcgac cccggcacga tgaatccgtt ccagcacggc gaggtgttcg
    10801 tgaccaacga cggggcggag accgacctcg acatcggaca ctacgagcgc ttcctcgacg
    10861 tcgacctgaa ccagcaggcc aacgtcacca ccgggcaggt gtactcgacc gtgatcgcga
    10921 aggagcggcg cggagactac ctcggtgaca ccgtccaggt gatcccgcac atcaccaacg
    10981 agatcaagca acgcatccgg gcgatggcgg ggcctgacgt cgacgtcgtc atcaccgaga
    11041 tcggtggaac ggtcggcgac atcgagtccc tcccgttcct cgaggccgct cgtcaggtgc
    11101 gccacgacat cgggcgtgac aactgcttct tcctgcacgt ttcgctggtg ccgtacatcg
    11161 gcccttccgg agagctcaag acgaagccga cccagcactc ggtcgccgcg ctccgttcga
    11221 tcggtatcgc cccggacgcc gtcgtctgtc gggccgaccg cgagatcccg gcgaacatca
    11281 agcgcaagat ctcgctgatg tgtgacgtcg acgacgaggc cgtggtcgct gccgtcgacg
    11341 cgccatcgat ctacgacatc ccgaaggtgc tgcacgccga gggactcgac gcgtacgtcg
    11401 tccgccgtct caacctgccg ttccgcgacg tggactggag acgctgggac gaactgctcc
    11461 aacgcgtcca ccaccccgac gaggaggtca ccgtcgcgct cgtcggcaag tacatcgatt
    11521 tgccggacgc ctacctctcg gtcgcggagg cactccgggc cggcggcttc gagcattcgg
    11581 cccggatcaa cctgcgctgg gtggcctctg acgagtgcca gacgcccgaa ggggcgtccc
    11641 gcctgctcgg cgacgtcgat gccgtctgcg tgccgggcgg tttcgggata cgcggcatcg
    11701 agggaaaggt cggtgcgctg acgtatgccc gcgagcaccg tatccccacc ctcggactct
    11761 gcctcggcct ccagtgcatg gtcatcgagt tcgcgcgcaa cgtcgcccac ctcgtcgaag
    11821 cgggttcgac ggagttcgac atcgacactg cgcagccggt catcgccacc atggaggagc
    11881 agctcgacat cgtcgacggt gcaggcgaca tgggtggcac gatgcggctc ggtttgtatc
    11941 cggccgacct tcagccgggc tccatcgctg cggaggccta cggctcgacg cgtgtcgagg
    12001 aacgtcatcg gcaccggtac gaggtcaaca acaagttccg gggcgacctc gaggacgccg
    12061 ggctggtctt ctcggggctc agtcccgacc acgagctcat cgagttcgtg gagctcccac
    12121 gcgaggttca tccgtactac gtctccacgc aggcccaccc cgagctgcgt tcccgcccga
    12181 cccggccgca cccactgttc agcgggctgg ttgccgctgc gctggagcgg caacgcgagc
    12241 tgcggctgcc cgtcgatgag acggcgctgc ggtcgacggc gtactggccc gcaccccaac
    12301 ccatcgagtg aaggcagccc gtcgagtgaa ggccgaagga cgacccgccg atacgccgga
    12361 gtcctggccg gtcctgtcga cgacgaccga gagcgagggt gcgatcgtct cggtgttctc
    12421 cgaggagatc gaggcgcctg acggcggccg gttcgtccgc caggtggtcc gccacccggg
    12481 cgcggtcgcg gtggtcgcgg tcgacagtga ggatcgggtg ctcgtgctgt cgcagtaccg
    12541 tcatccggtg aggcaacggc tggtggagct cccggccgga ctgctcgacg tcgacgggga
    12601 ggaccccgag agcgcagcag tccgggagct ggccgaggag gcgcagctgt ccgcgcgaca
    12661 ctggtcggtg ctcgtcgacg tgctcacctc accggggatc cacgacgagg cggtgcggat
    12721 cttcctggct gaagagctgt cagagatcga cctgcccgca ggattcgagg ccgagcacga
    12781 ggaggccgac atgagccggc actgggttcg gctggacgag ctggtgaccg cgatcctgga
    12841 cggggaggtc aaggacagtc tcacggtgtc gggatcgctt gctctctggg cccgtcgcca
    12901 cggatacggg agtggaaagc aactgtgact tcgaatatcg ttgcatcgcc cgcgatgatc
    12961 aggaccacgc gtcggaaaga gtgaggacaa tgaaggtcgg cgtacccaag gaagtcaaga
    13021 accacgagta ccgcgtcgcc atcacgcccg ccggcgtgca cgagctgagc aggcgcggcc
    13081 acgaggtgta cgtccaggcc agtgcgggga tcggcagctc gatcaacgac gacgagttcg
    13141 tcgccgccgg cgcgaagact cttccgaccg ccgacgacgt ctgggagacc ggtgacctca
    13201 tcttgaaggt gaaggagccg atcgccgagg agtatcaccg gatgcgtgac gggcagacgc
    13261 tcttcaccta cctccacctc gccgcgtcgc gggactgcac cgacgcactg atcaagcagg
    13321 gtgtcacggc gatcgcgtac gagaccgtgc agacgccgga cggcgcgctc ccgctgctcg
    13381 cgccgatgag cgaggtcgcc gggcggctcg cgccgcagtg cggcgcctac cacctgatgc
    13441 ggcagggcgg cggtcgaggc gtgctgatgg gcggcgtgtc cggcgtgtac gccgcgaagg
    13501 tcgtcgtgat cggtgccggc gtgtcgggca tgaacgccgc cgcgatcgcg ctaggcatgc
    13561 aggcggaggt gctgctcctc gaccgcaaca tcgagcggct gcgccaggcc gaccgcatct
    13621 accagggcca cctgcagacg gtggcgtcca acagctacga gatcgagcgc gccgtcatcg
    13681 acgcggacct ggtcatcggc gcggtacttg tcccgggagc gaaggcgccg atgctcatct
    13741 cgaacgagct ggtctcgcgg atgaagcccg gctcggtgct cgtcgacatc tcgatcgacc
    13801 agggcggctg cttcgaggac tcgcggccga ccacgcacgc cgagccgacg taccgtgtgc
    13861 acaactcggt cttctactgc gtggcgaaca tgccaggtgc ggtgccgcac acctcgacat
    13921 acgcgctgac gaacgtcacc ctcccgtacg ccgtctcgct ggccgacaag ggctggaagc
    13981 aggcgctgct cgacgaccac tcgctcgcac tcgggctcaa cacccatcag ggtgaggtca
    14041 cctacgagcc ggtcgcaacg gcccacggcc tggaccaccg gagtctcgac tccgtcctca
    14101 gctgacccag tcgcggagac aggcacgatc agcaccatgg tggccgagcc gtccaacctg
    14161 cgaggcgcca tccgcaccta tctggaccac ttggcggtcg agcgtggtct ggccgcgaac
    14221 accgtcgcgt cctaccggcg tgatctgcgt cggtatgacg gcttcctcgc cggccagggc
    14281 gtccgggccc tttcagacgt gaccgaaccg atggtcgcgg agttcctcat gcggttgcgc
    14341 gaaggggacg ccgcgcacca gccgctcagc gccggaagtg ccgcccgcac gctggtggcg
    14401 gtgcgtggct ttcagaagtt cgcggtccgg gagggcctcg ccgaccacga cccggcagcg
    14461 acggtgaagc cgccgacgcc ggcgaagcga ctgccgaagg cgcttccgct gtccgacgtc
    14521 gaagcgatcc tggcagcggc cggtgcgccg ggcacgacgc tggcgcttcg tgaccgggcg
    14581 ctgctcgagg tcctgtacgg cacgggagcc cggatatctg aggcggtcgg gctcgacatc
    14641 gacgacgtcg atctcgccga aggcgtggtc cggctgctgg ggaaaggcgg caagcagcgg
    14701 ctcgttccca tcggctccta cgcccgcgac gcactcgacg cctacctggt gcgtggccgg
    14761 ccgatgttgg ccgacggcgg tcgcggcacg cccgcggtct tcctcaacgc ccgcggtggg
    14821 cggctgtccc ggcagtccgc atggacggtg ctcggcaagg ctgccgaccg tgccggcgtc
    14881 acggccgatg tctccccgca caccatgcgg cactcgttcg ccacccacct gctcgacggt
    14941 ggcgccgacg tgcgcgtcgt ccaagagctg ctggggcacg cctcggtgac gacgacgcag
    15001 atctacacgc tggtgaccgt ggacaagctg cgcgaggtgt acgccgccag ccacccgagg
    15061 gcactgggct agcgggtacg gtccctcggc cgggtcgatg aatcgacttt tggcgcgcgc
    15121 cgcccgcgtg tcgtcgccag cggctgtgcc ggcccgtggt ggactgcaga tggacgtcga
    15181 acaatgagac tggagtggct gacgtgagcg agcccacctt cgtaccaccc ccgaaaaacc
    15241 cgctggcaac actgccaccc gaggcctccg ccaggcccgg gtcggcatcc atcacctcga
    15301 tcggctcgac gctggcgatg ggacagttca cgaccgtcgc cgagtcaccc cccgtgctcg
    15361 agatgggccc caccggcagg ccgctcccgg tgttcccaga gccgccgccg ctcatcgaac
    15421 acggcggtgc cacggtcatc gcgatgtgca accagaaggg cggcgtcggc aagaccacga
    15481 ccgtgatcaa cctcggtgcc agcctggcgg agtacggccg caaggtgctg ctcgtcgact
    15541 tcgaccccca ggcttcgctc accgtcggcc tcggcatcaa ccccaacgag ctcggcacca
    15601 cgatctacca cctgatgctc gaacgcgagg tcccgctcag cgatgtggtg gtacggaccg
    15661 gtgtcgaggg ggtggacctg atcccctcca acatcgactt cgccggcgcc gagatgcagc
    15721 tcatcaacga ggtcgcccgc gagcaggtgc tcgcccgcgc gctggcaccg ttcgtcgcgg
    15781 cgtacgacgt gatcctgatc gactgccagc cgtcgctggg cttgctgacc gtgaacgcgt
    15841 tggccgctgc ggacggggtg atcgtgccgt tggagtgcga gtacttcgct ttgcgtggcg
    15901 tcgcgctgct caaaggcacc atcgacaagg tgcaggaccg cctgaacccg aagctgcaga
    15961 tcatcggcct gctcgggacc atgtatgacg gccgcaccct gcacagtcgt gaggtgctcc
    16021 agaccctggt cgacggctgg ggcgacgcgg tgttccacac ggtgatccgg cggacggtga
    16081 agttctccga ggccacggtg gcgggagagc cgctgacggc gtacgcgacc gcctcgaacg
    16141 gtgccgactc ctaccgtcag ctggcccggg aggtgctgac ccggtgtcac gacgcgtaac
    16201 cctgcctggt gcggacgagc tgttcgggtc gaccgagaag ccgtctcggg cggagccgcc
    16261 gtcaccggcc gcgggcgacg gccagtcggc caccgggccg agcgggcgga tccgccatga
    16321 cgagaagatc accgtctacg tcaccgcgga cgagctgctg gatctcgagc acacccgcct
    16381 ctcgctgcgc cgcgaccacg ggctcgccgt cgatcggggc cgactcgttc gggaggcgat
    16441 ccatcttgcc ctcgaggacg cagccacgaa cggttccgac agcgcgctgg tacgccggct
    16501 gaccgcgtcg tgactgcacc gctgctcgac cgcgagtcgg aaagcgggtt cgcggtccat
    16561 ctcgacaact tcgagggacc cttcgacctg ctgctgcagc tcatcgccaa gcacaagctc
    16621 gacatcaccg aagtcgcgct gtcgaaggtc accgacgagt tcatcgtcca caccaaggcg
    16681 atgggtgccg actgggggct cgaccagacc accgagttcc ttgtcgttgc ggcgacgctg
    16741 ctcgacctga agatcgtccg gctgctgccg tcggctgagc tcgaggacga cgaggacctc
    16801 gccctgctgg aggc
//
LOCUS       DATBFZ010000056         2769 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_4942660, whole genome shotgun sequence.
ACCESSION   DATBFZ010000056 DATBFZ010000000
VERSION     DATBFZ010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2769
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4942660"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..975
                     /locus_tag="VH419_01665"
     CDS             <1..975
                     /locus_tag="VH419_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase family protein"
                     /protein_id="HEY7042353.1"
                     /translation="LTHRDMGPRARYLGPLVPKEVLIWQDPIPPVDHKLINDKDIAAL
                     KQKLLSSLSVSQLVSTAWASASTFRGSDKRGGANGARIRLSPQRDWDVNNPPELAKVL
                     KKLEAIHADFNKSAKGGKKVSLADLIVLGGDAAIEQAAKNAGVKVTVPFTPGRTDATQ
                     AQTDVESFSVLEPFADGFRNYQKARAPVSTEELLIDKAQLLTLTAPEMTVLLGGMRVL
                     NTNARPSKHGVFTKKTDALTNDFFVNLLDMDTEWKPTSDAKDVFEGRDRKSGQVKWTG
                     TRADLVFGSHSVLRAISEVYGSADAQEKFVKDFVAAWTKVMNLDRFDV"
     gene            1219..1749
                     /locus_tag="VH419_01670"
     CDS             1219..1749
                     /locus_tag="VH419_01670"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEY7042354.1"
                     /translation="MRMLAVVLFLSSTALAAEGAAKPATLKSSLLDQLKTTHDQQDWF
                     VPVRQAVDGVTAAQASWKPQGGDHSIGMLANHLLFWNSQNLARFKKVTPAKFDGNNDE
                     TFNAFDQAKWDEVRKRLDEVLTELEKLVAAADDKTLQSWAPTIGRIATHNAYHTGQIM
                     VLRKMQGAWDASKGVK"
     gene            1798..2469
                     /locus_tag="VH419_01675"
     CDS             1798..2469
                     /locus_tag="VH419_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017935105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY7042355.1"
                     /translation="MPRTMATYAFIHGAGDVGWYWHLVEAELRRRGHETISMDLPVED
                     DSAGLVDYAEVVASAIGGRPDPLIVAQSFGGYVAPIVCERVPARLLVLVAGMVPSPGE
                     SAEEMFVNTGYSTEPRSDGGQTDVFYDDVPPALAREAKARGRHQSDTPGKQPWPAKGW
                     PDVPTRFLLCRNDRLFPASWLRRVVRDRLSITPDEIDSGHTPALSRPKELAERLESYR
                     AGGSR"
BASE COUNT          483 a          889 c          976 g          421 t
ORIGIN      
        1 ctcacgcatc gcgacatggg tccgcgcgcg cgctatctcg gcccgctggt cccgaaggaa
       61 gtgctgatct ggcaggaccc gatcccgccg gtcgatcaca agctgatcaa cgacaaggac
      121 attgcggctc tgaaacagaa gctgctgagc agcctgtcgg tgtcgcaact ggtctcgacc
      181 gcctgggcgt cggcctcgac gttccgcggg tctgacaagc gcggcggcgc gaacggtgcc
      241 cgcattcgct tgagcccgca gagggattgg gacgtcaaca atccgccaga gttggccaag
      301 gtgctcaaga agctcgaggc gatccacgcc gacttcaaca agtcggcgaa gggcggcaag
      361 aaggtttcgc tggccgacct catcgtcctg ggcggtgacg cggccatcga gcaggcggca
      421 aagaacgccg gcgtgaaggt cacggtgccg ttcactcccg ggcggacgga cgcgacgcag
      481 gcgcagaccg acgtcgagtc gttctcggtg ctcgagccgt tcgcggacgg cttccggaac
      541 taccagaagg cgcgggcccc ggtgtcgacc gaggagctgc tgatcgacaa ggcgcagctg
      601 ctgacgctga ccgcgccgga gatgacggtg ctgctgggtg ggatgcgggt gctgaacacc
      661 aacgcccggc cgtcgaagca cggcgtcttc accaagaaga ccgacgcgct gaccaacgac
      721 ttcttcgtga acctgctcga catggacacg gagtggaagc cgacgtcgga cgcgaaggac
      781 gtgttcgagg gacgcgaccg caagtccggc caggtgaagt ggaccgggac ccgggcggac
      841 ctggtcttcg gctcgcactc ggtgctccgc gcgatctcgg aggtgtacgg gagcgcggac
      901 gcccaggaga agttcgtgaa ggacttcgtg gcggcctgga ccaaggtgat gaacctggac
      961 cgcttcgacg tgtgagcggt ggacgttccg ttcacgctcg gctcgggccg ggcgtgagcg
     1021 gtggcggtct cgcggcatga ctcgaccgcg gcgcggacgc cgtgctccgg tcttttttcg
     1081 gcacgccggc ttggcggatg ttgcggtccg cgcagtcaat ggcgcacggg aagccgccgg
     1141 ccggaccgag gctccacgcg ccctggacta ctctcctggt ggatggccct ccctggacga
     1201 gggtcgcgga ggatcccgat gagaatgctc gcggtcgtgc tgttcctctc ctcgacggcg
     1261 cttgctgccg agggcgccgc gaagccggcg accctgaaga gcagcctgct cgaccagctg
     1321 aagaccaccc acgaccagca ggactggttc gtcccggtga ggcaggcggt ggatggagtc
     1381 acggcggccc aggcgtcgtg gaagccgcag ggaggcgacc actccatcgg gatgctggcg
     1441 aatcacctgc tcttctggaa ctcgcagaac ctcgcgcggt tcaagaaggt cacgcccgcc
     1501 aagttcgacg ggaacaacga cgagacgttc aacgccttcg accaggcgaa gtgggacgag
     1561 gtccggaagc ggctggacga ggtcctgacc gagctggaaa agctcgtcgc ggccgcggac
     1621 gacaagacgc tccagtcctg ggcgccgacg atcggcagga tcgcgaccca caacgcgtac
     1681 cacacgggcc agatcatggt tctgcgcaag atgcagggag cgtgggacgc gtcgaagggc
     1741 gtgaagtaga gctcgacgag acaggcgctc tggcgacgcc ggaccatcgt gcgcagagtg
     1801 ccccgcacga tggccaccta tgcgttcatt cacggcgccg gcgacgtcgg ctggtactgg
     1861 cacctggtcg aggcggagct ccgcaggcgc gggcacgaga cgatctcgat ggatctcccg
     1921 gtcgaggacg actcggccgg cctggtcgac tacgcggagg tcgtcgcctc tgccatcggc
     1981 ggtcgcccgg atccgctgat cgtcgcccag tccttcggcg ggtatgtcgc gccgatcgtc
     2041 tgtgagcggg tgccagcgcg tctcctcgtc ctcgtcgccg gcatggtccc gtcgccgggc
     2101 gagtcagctg aggagatgtt cgtcaacact ggctattcga cggagccgcg gagcgacggg
     2161 ggccagaccg acgtcttcta tgacgacgtt ccgcccgccc tggcacgcga ggcgaaggcg
     2221 cgcggacggc accagtcgga cacgccgggc aagcagccct ggccggcgaa ggggtggccc
     2281 gacgtgccga cgcggttcct gctgtgtcgg aacgatcggc tgttcccggc gtcctggctc
     2341 cgacgggtgg tccgggaccg actgagcatc acgccggatg agatcgacag cggccacacg
     2401 ccggcgctga gccgcccgaa ggagctggcc gagcgactcg agtcctatcg agccggcggc
     2461 agcaggtagc tcagcccgat ggtcagcgac gagggtcgac gcactcgcgc caggcgaggt
     2521 gcgcgtggcc gccgcggtac tcccgaaact gacgttcaca gttcttcacc ggaatggctg
     2581 cggacaccct ccgtaagacg atcgaacgac gtcgccgctc cgccacagcg ggcgtcgatg
     2641 agctccggcc atctctcgaa cgtaatctcc tgctggcgaa cccgagcccg caaggagcag
     2701 gcatgactcg cacgacgaat gcaacgctcg ctggtttcac ctcgctcttt tacatggggg
     2761 tgggaatcg
//
LOCUS       DATBFZ010000057         3707 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_1350964, whole genome shotgun sequence.
ACCESSION   DATBFZ010000057 DATBFZ010000000
VERSION     DATBFZ010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 3707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3707
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1350964"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="VH419_01680"
     CDS             complement(<1..223)
                     /locus_tag="VH419_01680"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015071008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEY7042356.1"
                     /translation="MPVRCAWVPEGDELYAAYHDGEWGVPSRDPRHLFEMLILEGAQA
                     GLSWSTILRKREGYRLAFAGFDPAKVARLD"
     gene            complement(294..2576)
                     /locus_tag="VH419_01685"
     CDS             complement(294..2576)
                     /locus_tag="VH419_01685"
                     /inference="COORDINATES: protein motif:HMM:NF044618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M6"
                     /protein_id="HEY7042357.1"
                     /translation="MRKTRLVALLGVLVGVLVIVPSALSTRAPKGVAATPLDVLHVGP
                     DVSGLQVDLGVQQGRTAPVQPVGKAPVVGETRNWVGLDDTYGLLYRKQYTLRGVGNHI
                     EVWVASELNRRAPSSTLIPQVGQSSGTNFLDGDCRNGVRTTITDDQVNYLIGQFDDNI
                     YPKESATFSVPPDRDGSNAQVGAPFDPTGDGGHIVVLVDNVRDDNFYDLNNTQGFSYI
                     AGFFSSQINDLFDRNVMTIDAFDWLHRTGANPPNDPVPGNNCTSAPARPFLYEGTFAH
                     EYQHLLEHYEDSDEVNWVNEGLSDWAQTLTGYVDPSIPITQTGFDSHTQCFLGWLGVQ
                     TPANPNPREGGPENSLTEWGDQGDDEILCDYGAAYTMMEFLSGRYGTDFMTRLHRNDD
                     NGLVGLQAVLKQESSKLQAQDVLHDWSLMVALDGLIDNGAKIAGHPREKLVTTPTLHA
                     TINWDTPEAYSTPGAPPNGADYVRLRKASGTYFTGNEITSIGFTGATTLPTQPVQWTV
                     DQNPPTQPGDPALYSGADDNRDEAIVRQVAVPTGSGAELTFNAFWNEEEGWDFGFVQV
                     STDQGVSYQSLACTDTTTVTNPDAFPTAKENVPGFTGFSDGFRAQTCSLSAYAGQTVL
                     LAFRAFNDEATLGTDETVTPGFWVDDVKVGSTLISDGSSLTGWQSFTQVRPNPVSNFT
                     VRLISMKTGVKKTTIKVRELQLTGDFNLRGKARVQRYIDKNADFVAAVVFYDDPSETS
                     TQYAPYRLTVNGITQPGGGL"
     gene            complement(2655..>3707)
                     /locus_tag="VH419_01690"
     CDS             complement(2655..>3707)
                     /locus_tag="VH419_01690"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012923158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEY7042358.1"
                     /translation="NANEVIATLAGEGVHPNDHVNMGQSSNDVFPSAVHLAALDELVN
                     DLLPALVRLGDSLEAKAKEFDDVVKSGRTHLMDAVPVTLGQEFAGYAAQVRQGAKRVE
                     ATFERLGQVPLGGTATGTGLNTHPEFAERVRVRVRESTGLPIAAPADPFEAQAARDAL
                     VEASGALKTVAVSLTKIANDLRFMGSGPRAGLAEISLPELQKGSSIMPGKVNPVIPEV
                     VTQVAAQVIGNDAAIAIGGMQGHFELNVFVPLIKRNLLDSIKLLASASRLLAEKCVDG
                     IEANRETNEHYAELTLSAATALNPYIGYDKATEIVKEAATSGRPLRDVARDAGVEESV
                     LDEALDYRKMAKPHPG"
BASE COUNT          532 a         1225 c         1301 g          649 t
ORIGIN      
        1 gatcgagccg ggccaccttc gccgggtcga aaccggcgaa cgcaagccgg tagccctcgc
       61 gcttgcgcag gatcgtcgac cacgacagcc cggcctgagc tccctccagg atcagcatct
      121 cgaacaggtg gcgggggtcg cgcgagggca cgccccactc cccgtcgtgg taggcggcgt
      181 acagctcgtc gccctccggg acccaggcgc agcggaccgg caccggcgta tcgaaccaga
      241 caggcgaggg gccgcccgga ggcggcccct cgagccgagt tgcgaccgga cggctagaga
      301 ccgccgccgg gctgcgtgat gccgttgacc gtgagccggt acggcgcgta ctgcgtgctc
      361 gtctcggacg ggtcgtcgta gaagacgacc gccgcgacga agtccgcgtt cttgtcgatg
      421 tacctctgaa cgcgcgcctt cccacgcagg ttgaagtcgc ccgtgagctg gagctcgcgg
      481 accttgatcg tcgtcttctt cacgcccgtc ttcatgctga tcagccggac ggtgaagttc
      541 gagaccgggt tcggacggac ctgggtgaag gactgccagc ccgtgagcga cgacccgtcg
      601 gagatcagcg tgctgccgac cttgacgtcg tcgacccaga agccgggggt caccgtctcg
      661 tccgttccga gggtggcttc atcgttgaag gcccggaagg cgagcagcac ggtctggccc
      721 gcgtaggcgg acaggctgca cgtctgggcc cggaagccgt cggagaagcc ggtgaagccc
      781 ggcacgttct ccttggccgt cgggaacgcg tccggattcg tcaccgtggt cgtatccgtg
      841 caggcgaggc tctggtagct gacgccctgg tcggtcgaga cctggacgaa gccgaagtcc
      901 cagccctcct cctcgttcca gaaggcgttg aaggtgagct cggcgcccga gccagtgggc
      961 accgcgacct gccggacgat cgcctcgtcg cgattgtcgt ccgcaccgga gtacagcgcg
     1021 ggatccccgg gctgtgtcgg tgggttctgg tcgaccgtcc actgcaccgg ttgggtgggc
     1081 aaggtcgtcg ccccggtgaa gccgatcgag gtgatctcgt tgcccgtgaa gtacgtcccg
     1141 gatgccttcc gcagccggac gtagtcggcg ccgttcgggg gcgcacccgg cgtcgaatac
     1201 gcctccgggg tgtcccagtt gatcgtcgcg tgcagcgtgg gcgtcgtcac cagcttctca
     1261 cgcgggtggc cggcgatctt ggcgccgttg tcgatcaggc cgtcgagcgc gaccatcagg
     1321 ctccagtcgt ggaggacgtc ctgcgcctgg agcttgctcg actcctgttt cagcacggcc
     1381 tggagaccga ccaggccgtt gtcgtcgttc cggtgcaggc gggtcatgaa gtccgtcccg
     1441 taccggcccg acaggaactc catcatcgtg tacgcggcgc cgtagtcgca gaggatctcg
     1501 tcgtctccct ggtcacccca ctcggtcaac gagttctccg gcccgccttc gcgtggattg
     1561 gggttcgccg gcgtctgcac gccgagccag cccaggaagc actgcgtgtg cgagtcgaag
     1621 ccggtctgcg tgatcgggat cgacgggtcc acgtagccgg tgagtgtctg ggcccagtcc
     1681 gacaggccct cgttcaccca gttgacctcg tcgctgtcct cgtagtgctc gagcaggtgc
     1741 tggtactcgt gcgcaaacgt gccctcgtac aggaacggcc gcgccggggc gctggtgcag
     1801 ttgttgcccg gcaccgggtc gttcggcggg ttcgcccccg tccggtgcag ccagtcgaac
     1861 gcatcgatcg tcatcacgtt gcgatcgaag aggtcgttga tctgtgagct gaagaagccc
     1921 gcgatgtacg agaagccctg cgtgttgttc aggtcgtaga agttgtcgtc gcggacgttg
     1981 tcgaccagga cgacgatatg gccgccgtcg ccggtcgggt cgaacggcgc accgacctgt
     2041 gcgttcgagc cgtcgcggtc gggcggaacg ctgaaggtgg ccgactcctt cggatagatg
     2101 ttgtcgtcga actggccgat caggtagttc acctgatcgt cggtgatcgt cgtgcgcacg
     2161 ccgttacggc agtcgccgtc caggaagttc gtgcccgagg actggccgac ctgcgggatg
     2221 agtgtcgagg acggagcgcg gcggttgagc tcgctcgcca cccagacctc gatgtggttg
     2281 cccacgccgc gcagcgtgta ctgcttgcga tagagcagac cgtaggtgtc gtcgagcccg
     2341 acccagttgc gcgtttcgcc gacgacaggt gccttgccga ccggctggac cggtgccgtc
     2401 cttccctgct gaacgccgag atcgacctgc agcccgctga catccgggcc gacgtgcagg
     2461 acgtcgagcg gcgttgcggc cacgcccttc ggcgccctcg tcgagagagc gctcggaacg
     2521 atcacgagca ctccgacgag aacgcccagc agcgcgacga gtcgcgtctt gcgcatctac
     2581 ttcccccctg tgtcgattgc ctgaggcccc tgccgcccgg ggctggacct ctctatgtcc
     2641 gcggtgagag atccctaacc agggtgcggc ttggccatct tgcggtagtc gagcgcctcg
     2701 tccaggacgg actcctcgac cccggcgtcg cgggcgacgt cgcggagcgg gcgcccggac
     2761 gtcgccgctt ccttgacgat ctcggtcgcc ttgtcgtagc cgatgtaggg gttgagggcg
     2821 gtcgccgcgg agagcgtcag ctcggcgtag tgctcgttcg tctcgcggtt cgcctcgatg
     2881 ccgtcgacgc acttctcggc gagcagacgc gaggcggagg cgagcagctt gatcgagtcg
     2941 agcaggttgc gcttgatcag cgggacgaag acgttgagct cgaagtggcc ctgcatgccg
     3001 ccgatcgcga tcgccgcgtc gttgccgatc acctgcgcgg cgacctgcgt gacgacctcc
     3061 gggatcaccg ggttgacctt gcccggcatg atcgagctcc ccttctggag ctcgggaagg
     3121 gagatctccg ccaggccggc ccgcgggccg gagcccatga agcgcaggtc gttggcgatc
     3181 ttggtcagcg agacggcgac ggtcttcagc gctccggagg cctccacgag cgcgtcgcgc
     3241 gccgcctggg cctcgaacgg atcggccggc gcggcgatcg gaagcccggt cgactcgcgc
     3301 acacggacgc ggacgcgctc cgcgaactcc gggtgcgtgt tgagtcctgt ccccgtggcg
     3361 gtgccgccga gcgggacctg gccgagccgc tcgaacgtcg cctcgacccg cttcgcgccc
     3421 tggcggacct gcgctgcgta gccggcgaac tcctgtccga gcgtgacggg gacggcgtcc
     3481 atcaggtgcg tgcggcccga cttgacgacg tcgtcgaact ccttcgcctt ggcctcgagc
     3541 gagtcgccga ggcggacgag cgcgggcagc aggtcgttga cgagctcgtc gagcgcggcg
     3601 aggtggaccg cggacgggaa gacgtcgttc gacgactggc ccatgttcac gtggtcgttc
     3661 ggatggacgc cctcgccggc cagcgtcgcg atcacctcgt tggcgtt
//
LOCUS       DATBFZ010000058        20244 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_2335985, whole genome shotgun sequence.
ACCESSION   DATBFZ010000058 DATBFZ010000000
VERSION     DATBFZ010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 20244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20244
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2335985"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="VH419_01695"
     CDS             complement(<1..239)
                     /locus_tag="VH419_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020384734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY7042359.1"
                     /translation="MPGRPGGAGAAEVAAHQPTTPDAIRNVVLVGPPGSGKTTLVEGL
                     LHATGVINRMGRVEDGTTVSDYDEAEIRQQRSIGLS"
     gene            524..1144
                     /locus_tag="VH419_01700"
     CDS             524..1144
                     /locus_tag="VH419_01700"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017933627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEY7042360.1"
                     /translation="MLERFRQFWTNLFKPISHGLIKLGVSPDAVTIVGTIGVVVGALG
                     FFPRGELLIGVIVITVFVFSDMIDGYMARTLGVSSKWGSFLDSTLDRIGDAAIFAGLA
                     IWLFQGGDNEVLAYVALWCLVMGSVTSYARAKAESLGMQAKGGIAERSDRLVAVLVMT
                     GLSDMLDVPFLLTLVLWVLAVASTITVVQRMVLVRRQALADPALPS"
     gene            1156..2097
                     /locus_tag="VH419_01705"
     CDS             1156..2097
                     /locus_tag="VH419_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020384739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylinositol mannoside acyltransferase"
                     /protein_id="HEY7042361.1"
                     /translation="MVTFARSGPVPGADRRRAPAAREALVDHAFGIGWAITRHAPEPV
                     AERLLEHAADRITRQRGSGVEQLERNLARAAPELDADDLRELTQTAMRSYFRYWHEAF
                     RLPSWTRERVVDGVVTTNETPLREGFARGQGAIVALPHMANWDLAGAWACLTGMPVST
                     VAERLRPESLYERFVAYRRQLGMEVIALTGEGSPLRALREALLRGRLVCLLSDRDLSG
                     NGVEVDLLGEPACLPGGPAVLARMTGAPLVAASLCFSGPLLRIDFSDPVPVRPGPSGL
                     AAMTQDVADWFTAGVRRNPADWHMLQPVFVGDRGLRR"
     gene            2094..3197
                     /locus_tag="VH419_01710"
     CDS             2094..3197
                     /locus_tag="VH419_01710"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012920984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY7042362.1"
                     /translation="MRVGIVCPYSFDVPGGVQNHVRDLAVALHAQGHEVGVLAPGECA
                     DGLPSYVELVGKAVPVPYNGSVARLAFGPRVASRTARWLRTGDFDVVHVHEPIAPSVS
                     LLTLWTAEQPLVATFHTAIERSRAMASVSPLVRPSLEKLAAQIAVSEVARDTLVHHLG
                     GGPVVIPNGLFCDAFASGGPEPVRHRTEPVVVFLGRIDEPRKGLHVLLDAWPQVTAVF
                     PTARLLVAGRGDRLGLAPSGPLTGVEFLGQVSDDARGRLLRSATVCVVPALGGESFGI
                     VLVEAMAAGAAVVASDLPAFRSVLAGDCGRVFPPGDSAALAETIVDVVSDATQRHAMT
                     ERASIAVRRFDWSRVIDQVVTVYDTATRAMATR"
     gene            3399..4325
                     /gene="pdxS"
                     /locus_tag="VH419_01715"
     CDS             3399..4325
                     /gene="pdxS"
                     /locus_tag="VH419_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HEY7042363.1"
                     /translation="MTSEIQNQPTPAPSSPETGTAKVKRGMAEMLKGGVIMDVVTSEQ
                     AKIAEEAGAVAVMALERVPADIRAQGGVSRMSDPDMIDGIIDAVSIPVMAKARIGHFA
                     EAQVLQSLGVDYIDESEVLTPADYANHIDKWQFTVPFVCGATNLGEALRRITEGAAMI
                     RSKGEAGTGDVSNAVTHMRTIRAEMRRLQSMSEDELFVAAKELQASYDLVREVATLGR
                     LPVVLFTAGGIATPADAAMMMQLGAEGVFVGSGIFKSGNPAQRAAAIVKATTFYDDPD
                     VVAKVSRGLGEAMVGINVDEIPQPHRLAERGW"
     gene            4339..4929
                     /locus_tag="VH419_01720"
     CDS             4339..4929
                     /locus_tag="VH419_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042364.1"
                     /translation="MAIGSLDKAGRLEQLKTYARVQVWSGYRSPADVRSDVYDALLDE
                     VKDPSEAERLTSDYLVQAERDLESAAAEWPDPSAFDRLQATFEDLREQGLIVLEACED
                     HWSANAALETAGAEGPEPAGIAYFTHADVWHAVEHGMLELNVWHPSTANVAPGDPLLE
                     RVQEALARHRISSVFDEGRIEVSVAWERRPARPHSA"
     gene            5212..5670
                     /locus_tag="VH419_01725"
     CDS             5212..5670
                     /locus_tag="VH419_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042365.1"
                     /translation="MKKLTRRTAAVGVVTLGLVGAGVAFAAWTSTGTGSGSAEATSAV
                     DLQVHVDPATGLYPTGSKNATFTVTNQNPYAVRLITAEAATDFSVDAAHSTCNLDSLH
                     SAAQMLNDVIPSGQTSAPHTVALSMDNNANDACQGATFGFKLTVHGASSS"
     gene            5726..6301
                     /locus_tag="VH419_01730"
     CDS             5726..6301
                     /locus_tag="VH419_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042366.1"
                     /translation="MTERRRTRRTLVLLLALIGLTATGATHASLGPSEPDPSVFVGNG
                     VGEAMPRGAAGTSSLQPATPTSYVGVNGHGSGHRGVSGHDWWRGPISVAVGTVSGLYP
                     GLTKRLPVTVTNPNSFGIGVTRIKVRTLGTVTCSGHSFTARHSSLVRQRFIRATRTRT
                     FAIPYRMRRTAPDGCQRAHVAIRVRVRAVHR"
     gene            6298..7317
                     /locus_tag="VH419_01735"
     CDS             6298..7317
                     /locus_tag="VH419_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042367.1"
                     /translation="MRRLLIVAALVAVGLAVQPAMAMAEWLSPGTGSGSSTATRVNSP
                     VAPTVVRSGSNVVVSWPQGKLANGTVVTGYQVKRTVGTTTTTLCTTTEPTRTCTDGNP
                     AGTASYAVVNRYANWSATGPTTSLTFDQTAPATTLTSNPAPNAAGWNKTDPTLTLTAT
                     DAGGSGVASITYKIGSGSAVTVNGASASFPVTTTGVTTVTYFATDVAGNVEATKTSTV
                     RLDKTSPTLTNVFPANNGSYTNQQYRIGCTPQRSVCGNANDNQQVVTVQIKLMNSSGQ
                     CLGSGGGFTTANCSAYLTATGTTTWYSTTGNLAKSTYTLTVLVTDVAGNTNGPAGQTF
                     AFTRS"
     gene            7374..7994
                     /gene="pdxT"
                     /locus_tag="VH419_01740"
     CDS             7374..7994
                     /gene="pdxT"
                     /locus_tag="VH419_01740"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015582857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HEY7042368.1"
                     /translation="MPRTSSPTIGVLALQGDVREHLRMLSGVGATPVAVRRPSELAAV
                     DAIVLPGGESTTMHKLARVFGLFEPLQRRLSDGMPALGTCAGMILLADRIESGIDGQE
                     TLGGLDVVVRRNAFGRQVDSFEVDVDFEPFDRPFHAVLIRAPWVEKVGDGVDVLATVA
                     GGPAAGRVVAVRQGNIMATSFHPEITSDDRVHHYFADLVRNSRGAQ"
     gene            7997..8773
                     /locus_tag="VH419_01745"
     CDS             7997..8773
                     /locus_tag="VH419_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020660186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY7042369.1"
                     /translation="MSGHSKWATTKHKKAVIDARRGKLFAKLIKNIEVAARTGGGDPA
                     GNPTLYDAIQKAKKSSVPNDNIDRAVKRGSGLEAGAADYQTIMYEGYAPGGVALLIEC
                     LTDNRNRAAGEVRTAMTRGGGSMADAGSVSYMFARKGVVIVPKAVDNQEGGATELDED
                     TVLAAVLDAGAEEVNDLGESFEVVSEPTDLVAVRTSLQDAGLDYESAEAAFVPSITVD
                     VDKDVAGRVFRLIETLEDSDDVQNVFSNVDVSDDILAQLA"
     gene            8970..9506
                     /gene="ruvC"
                     /locus_tag="VH419_01750"
     CDS             8970..9506
                     /gene="ruvC"
                     /locus_tag="VH419_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008356869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEY7042370.1"
                     /translation="MRVLGVDPGLTRCGLGVVEGGFSQPLRAIAVDVVRTPSDADLGA
                     RLVAIEREVEAWLDAYAPDAVAVERVFSQHNVRTVMGTAQASGVAVVAAARRGLPIAL
                     HTPSEVKAAVTGSGRAGKEQVTAMVTRVLRLESRPTPADAADALALAMCHIWRGQAGG
                     RLDEALAKAAGGSRSGDR"
     gene            9503..10126
                     /gene="ruvA"
                     /locus_tag="VH419_01755"
     CDS             9503..10126
                     /gene="ruvA"
                     /locus_tag="VH419_01755"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEY7042371.1"
                     /translation="MIAFVKGTVSAVFVDSAVVDVGGVGVLVMCTPAALATLRVGDEA
                     TLATSMVVREDSLTLFGFADYDERSLFELLQTASGVGPKLAQAMLAVHGPDELRRAVM
                     TEDLVALCKVPGIGRKGAQRIVLELKDRVGAPAGGAVPTHAPARVAADGWRDQVRAGL
                     LGLGWSVKEAEAAVDAVAPMAAEALSDGETAPVAELLRAALQSLSRA"
     gene            10173..11225
                     /gene="ruvB"
                     /locus_tag="VH419_01760"
     CDS             10173..11225
                     /gene="ruvB"
                     /locus_tag="VH419_01760"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012921990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEY7042372.1"
                     /translation="MDEPAGRGLVTADADGDERTLEAALRPRTLAEIIGQERVREQLG
                     LVLSAARGRGRCPDHVLLSGPPGLGKTTMAMIIATEMGAPLRVTSGPAIQHAGDLAAI
                     LSGLGEGEVLFLDEIHRMSRPAEEMLYMAMEDFRVDVVVGKGPGATAIPLEIPVFTLV
                     GATTRAGLLPGPLRDRFGFTGHLDFYDVDELQQIVNRSARLLEVEVDAGGANEIASRS
                     RGTPRIANRLLRRVRDFAQVRSDGRVSRDLAHDALALFEVDESGLDRLDRAVLDALCR
                     RFGGGPVGLGTLAVAVGEERETVEEVAEPFLVRSGYLARTPRGRVATPAAWHHLGMTA
                     PRAAPYQDQEALFGDR"
     gene            11405..11791
                     /gene="yajC"
                     /locus_tag="VH419_01765"
     CDS             11405..11791
                     /gene="yajC"
                     /locus_tag="VH419_01765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEY7042373.1"
                     /translation="MSGNALAQLLPLLLLVVLVYFLMIRPARRRAQQVSRLQAALSVG
                     DEVMLSSGIFGTVESVFDDRVNVKVADGVVLVVHRGAVNEIVRDVPAESATAADGEPG
                     GAEDQRLPEQDPDDPGTHEDTTRGAN"
     gene            11793..13598
                     /gene="secD"
                     /locus_tag="VH419_01770"
     CDS             11793..13598
                     /gene="secD"
                     /locus_tag="VH419_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017933619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEY7042374.1"
                     /translation="MAAKTSHPTRTLLIFMLVIGGLYGAAAAVGTWKPKLGLDLQGGT
                     RITLQAKSTGGGSVTSDKLDEAVDIISNRVNGAGVAESEVSTQGSDIIVVEIPGKVDK
                     NLERTIGSTAQLRFRLEALRLPPAGTTLPSPTDTGATSPSGSEPTSPKSTTGNESPAT
                     PSGTRTGPTIKPKPGDKTPNTTSDTGKNRAGAAWMRSGNNAPSTPTEPTPSGPTTGPP
                     TELVPPDLEDIKDPYAWVANPPQEWQARAAAYVCPGPGQPSSGQIDLPSQPLIACDEA
                     GNRFLLGPAMVEGTHVKDASYGIPQQGIDYVVNVSFDGEGSKQFADVTTRIAGQTNPL
                     NGDKQRLAIVLDGEVLSDPSNDQPITGGRAEISGNFDQDQAKSLANSLKYGALPLTFV
                     VQSRTDEGPELASNQLTAGIIAGIIGLILVVIYCLIYYRGLGFVVIASLIIAGLLTYA
                     CVLLLSHAYGFTLTLPGIAGLIVAIGITADSFIVYFERLRDEVREGKTLRTSVETGWR
                     RARTTILAADGVSILAALVLFIFAIGVVKGFAFALGLTTVVDIFVVFFFTKPMVTMLS
                     RTKFFGHGHKWSGLDRQHLGMPPLKKTPRTAGGEA"
     gene            13598..14773
                     /gene="secF"
                     /locus_tag="VH419_01775"
     CDS             13598..14773
                     /gene="secF"
                     /locus_tag="VH419_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEY7042375.1"
                     /translation="MSKFGRIGAALYRGDVSIDFVGRRRTWYKISLAILAIAVVALVW
                     RGLNFGIEFRGGVEFQAQISNPSSKVESVTNAVVDTGIEAAQSPVVVASNNDSIRVQT
                     EPLTLDEINVVSNALTDEGATEVSTASIGPTWGQAIAKKALTGLIVFVILVVLFIWAY
                     FREWRMSVAAIVALAHDLLITVGIYALTGFEVTPATVTGLLTILGYSLYDTVVVFDKV
                     RENTRNITASTRRTYAESANLAVNQTLVRSINTSIAALLPVGAILYVGAFMLGSGPLK
                     DLALALFVGMAAGAYSSIFIATPLLAQLKMREPAMQAQAKRVTSRRTAADRMIVHDPE
                     RAAAEAESAEQEDDGKPPAPRPVVTRTATKTAPRPTSSSASKRSQPQRQARSKRGKR"
     gene            14770..15321
                     /locus_tag="VH419_01780"
     CDS             14770..15321
                     /locus_tag="VH419_01780"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEY7042376.1"
                     /translation="MSAGAPDDLAALIAPLVRDVSDFPEPGIVFKDLGPLFADHDALT
                     RVVEAIADAGRADGRGRVDKVVGIESRGFILGAPVALELGAGFVPVRKQGKLPGPTFQ
                     RSYALEYGEATLEVQRDAFAPGDFVLVVDDVLATGGTVEATAALVEQCGAEVVGIAVL
                     IELGFLSGRDRAAGHAVTSLLIL"
     gene            15640..17883
                     /locus_tag="VH419_01785"
     CDS             15640..17883
                     /locus_tag="VH419_01785"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEY7042377.1"
                     /translation="MRTRLARIGRSSGFDPVLEPLFQVVRNTHPKADLAIIEKAYKAA
                     ERHHHGQTRRSGDPFITHPLAVTTILAELGMTPPTLCAALLHDTVEDTPYTLDQLRAD
                     FSDEIALLVDGVTKLDKVSYGDSAQAETIRKMIVAMSKDIRVLVIKLADRLHNMRTLR
                     FLRQATQERIASETLEIYAPLAHRLGMNTIKWELEDLAFATLHPKVYDEIVRLVAERA
                     PARDEFLATVIDQVQHDLRGAKIKATVTGRPKHYYSIYQKMIVRGREFTDIYDLVGIR
                     ILTDSLRDCYAVLGVLHARWNPVPGRFKDFVAMPKFNMYQSLHTTVIGPAGKPVELQI
                     RTQAMHRRAEYGVAAHWKYKEDGRAGLDTNRPLNADEGSGDMIWVRQLLDWQRETEDP
                     GEFLDSLRFEINSAEVYVFTPRGEVIALPTGSTPVDFAYSIHTEVGHRCIGARVNGRL
                     VPLESQLDNGDVVEIFTSKAQNAGPSRDWLTFVKSPRARNKIKQFFTKERREEAIERG
                     KETIARLMRREGLPLQRMLTHDTLSVVAGELRLPDVTALYAAVGEGNTTAQTVVRRIL
                     ELFGGDDAAADEAAESTVTITSERERRKRSPRGDAGVIVKGLSDVWVKLARCCTPVPG
                     DDIVGFVTRGSGVSVHRGDCTNIKSLSSQPGRMVEVEWAPTAQSMFLVAIQVEALDRN
                     RLLSDITRVLSDVHVNILSASVATTRDRIAKSRFTFEMADPTHLGHVLKAVRTVDGVF
                     DAYRVTQ"
     gene            complement(18144..19385)
                     /locus_tag="VH419_01790"
     CDS             complement(18144..19385)
                     /locus_tag="VH419_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014166942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HEY7042378.1"
                     /translation="MTETPDGGGFGRVDADGTVYVKTKDGERVVGQWPDGDPQAALDF
                     YRKRYEGLVVEVDLLEQRIKAGSLSPEDATATVAKVKASIQSAQAVGDLDALAARLDA
                     LEPVIAERREARRAERAARADEAKQAKRRIAEEAERLASGTDWRHGANRLRELLTTWK
                     SLPRIDKASDDELWHRFSSARTAYTRRRKQHFAELNERRDSAKVVKEKLVAEAEELAD
                     STDWGATARAYRDLMSRWKAAGPAQKDVDDALWKRFRAAQDAFFGARDAANAALDQQY
                     AANADVKRGLLVEAEALLPVRDPRTAREQFRELAEKWDAAGKVPRTEMKDLETRFKRV
                     EQAVRGAEDDQWRRRNPEAHARAADTVAQLEASIASLQSDLATAEASGDARQVESAKA
                     AITARQSWLDEAKRALTEFST"
     gene            complement(19382..19687)
                     /locus_tag="VH419_01795"
     CDS             complement(19382..19687)
                     /locus_tag="VH419_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042379.1"
                     /translation="MRDSRRQRTISIVVGVVIGLVAAALLAWLVVHIIDEENTRSPQA
                     PAVPTDTFKTDLLTPSTPPQTNGTQPTGGATNTPTQSNHKPTDNKPSSKATATRGNR"
     gene            19961..>20244
                     /locus_tag="VH419_01800"
     CDS             19961..>20244
                     /locus_tag="VH419_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017933613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY7042380.1"
                     /translation="MLIAAFPAGPWATNCYVVAPGPREECVVIDPGYDAASGVAAVVR
                     EHHLRPAAVLLTHGHVDHIWSVTPVAGAYDATAYIHPADRHLLTNPAAGLS"
BASE COUNT         3143 a         6930 c         6928 g         3243 t
ORIGIN      
        1 gagaggccga tcgatcgctg ctgccgtatc tcggcctcgt cgtagtcgga caccgtggtg
       61 ccgtcctcga cccgccccat ccggttgatc acaccggtgg cgtgcaggag gccttcgaca
      121 agcgtcgtct tgccggagcc tggcggcccc accaagacga cgttacggat cgcatcgggg
      181 gtggtgggct ggtgggccgc aacctcggct gcacccgccc ctcctggtct gcctggcatg
      241 aacgctgctc ctgtcgccga acttcgcttc cggtcgccca acgtcggtcg aacccgaacg
      301 tgcctccacc tttcaccaga cccgcccccg acacaaggcc ttcctccctc gccgaggtgg
      361 caactttcgt tcgagattcg cggcgtgtcg cgacgtcgtt gccacctcga tcgagggcgg
      421 ccgtcggttg aggatccgac cacgccggcc ggtacgatca ccagcagatc ggaaacgtcg
      481 tacgccgcgg cgaatcctcc acggaaaggg ctccaccaag cacatgctcg agcgcttccg
      541 tcagttctgg accaacctgt tcaagcccat ctcgcacggc ctgatcaagc tcggtgtctc
      601 gcccgacgcg gtcaccatcg tcgggacgat cggcgtcgtc gtcggggcgc tcgggttctt
      661 cccccgtggt gagctcctga tcggcgtcat cgtcatcacg gtgttcgtct tctcggacat
      721 gatcgacggc tacatggcgc gcacgctcgg cgtctcctcg aagtggggct cgttcctcga
      781 ctcgacgttg gaccggatcg gcgatgccgc gatcttcgcc gggctggcga tctggctgtt
      841 ccagggtggt gacaacgagg tgctcgcgta cgtcgccttg tggtgtctcg tgatggggtc
      901 cgtgacgtcg tacgcccggg ccaaggccga gagcctcggc atgcaggcca agggagggat
      961 cgccgaacgc tccgaccgac tcgtcgcggt cttggtgatg accggactgt ccgacatgct
     1021 cgacgtgccg ttcctgctca cgctcgtcct gtgggtgctg gccgtggcga gcacgatcac
     1081 ggtcgtccag cggatggtgc tggtacgccg acaagcgctg gccgacccgg cgcttccctc
     1141 ctgagcgcgt tcgtcatggt gacgttcgcc aggtccggac cggtgcccgg cgccgaccgc
     1201 cgacgtgcac cggcggcccg ggaggcactc gtcgaccacg cgttcggcat cggctgggcg
     1261 atcactcggc acgctcccga accggtggcc gagcgactgc tggagcacgc cgccgaccgg
     1321 atcacacgcc agcgcgggag cggggtcgag caactcgagc gcaacctcgc cagggccgca
     1381 ccggagctcg acgcggacga tctgcgtgag ctgacgcaga cggcgatgcg ctcgtacttc
     1441 cggtactggc atgaggcgtt ccggctgccc tcctggacaa gggagcgcgt cgtggacggc
     1501 gtcgtgacga cgaacgagac gccgctgcgc gaggggttcg cccgcggtca gggagcgatc
     1561 gtcgcgctgc cccacatggc caactgggat ctggcggggg cgtgggcgtg tctcaccggg
     1621 atgccggtgt cgacggtcgc ggagcgactt cgcccggagt cgttgtacga acgcttcgtg
     1681 gcctaccgac ggcagctggg catggaggtg atcgcgttga cgggggaggg cagcccgttg
     1741 cgagcacttc gcgaagcgct gctccgcggt cggctcgtgt gcctgctgtc ggaccgcgac
     1801 ctgagcggca acggcgtcga ggtcgatctc ctgggcgagc cggcatgcct ccccggcggt
     1861 ccggcggtgc tggcccggat gacgggcgca ccgctggtgg ctgcgagcct gtgcttcagc
     1921 ggtccgttgc tgcgtatcga cttcagcgat cccgtgcccg tccggccagg tccgagcgga
     1981 ctggcagcga tgacgcagga cgtcgccgac tggttcactg ccggcgtccg gcgcaatcct
     2041 gccgactggc acatgttgca gccggtgttc gtcggcgacc gtgggctgcg gcgatgaggg
     2101 tcgggatcgt ctgcccctac tccttcgacg ttcccggtgg cgtccagaac cacgtccgcg
     2161 acctcgctgt cgcgctgcac gcccaggggc atgaggtcgg tgtgctcgcc cccggggagt
     2221 gcgcggatgg cctgccgtcg tacgtcgagc tggtgggcaa ggcggtcccg gtgccgtaca
     2281 acggatcggt cgcccggctc gcgttcggac cgcgtgtcgc atcacgcacc gcccgctggc
     2341 tgcgtaccgg cgacttcgac gtcgtacacg tccacgagcc gatcgccccg agcgtctcgc
     2401 ttctcacgct gtggacggcc gagcaaccgc tggtcgcgac gttccacacc gccatcgagc
     2461 ggtcccgggc gatggcgtcg gtctcgccgc tggtgcggcc gagcctggag aagctcgctg
     2521 cccagatcgc ggtgtcggag gttgcccgtg acaccctcgt tcaccatctc ggcggtgggc
     2581 cggtcgtcat tccgaacggc ctcttctgtg atgccttcgc cagcggcggg ccggagccgg
     2641 tgcggcaccg gaccgagccg gtcgtcgtgt tcctcgggcg catcgacgag ccacgcaagg
     2701 gcttgcatgt gcttctcgac gcgtggccgc aagtcaccgc ggtgtttccg accgctcggt
     2761 tgctcgtcgc aggacgtgga gaccgcctcg ggcttgcgcc gagcggaccg ttgaccggtg
     2821 tcgagttcct gggacaggtg agcgatgacg cgcgaggtcg gctgctgcgg tcggccacgg
     2881 tctgcgtggt gcccgcgctc ggcggggaga gcttcgggat cgtgctcgtc gaggcgatgg
     2941 ctgccggtgc agcggtggtc gccagcgatc tgcccgcgtt ccgctccgtg ctggccggcg
     3001 actgtgggcg ggtcttcccg cccggcgact cggcggcgct ggcggagacg atcgtcgacg
     3061 tcgtctccga tgcgacgcaa cggcacgcca tgaccgagcg ggcgtcgatc gccgtacgcc
     3121 ggttcgactg gtcacgggtg atcgaccaag tcgtcaccgt ctacgacacc gcgacccggg
     3181 cgatggcgac gcgctgaccc tctcgcgtcc cttggcgccg ccgtccccgg aaatgcggga
     3241 ggttccgcga tttcgggcga ggaaatcgcg gaacctcccg caattcgcgg agaggtgagc
     3301 ccgtccggtg acaggtgcat cgcgctttgt ggctcctgac gcccgcgccg tagactgacg
     3361 gcaagtccac acctcagaaa ccccacgtaa ggctcatcgt gaccagcgaa atccagaacc
     3421 agccgacgcc cgccccgtcg agtcccgaga ccggcaccgc caaggtgaag cgcgggatgg
     3481 cggagatgct caagggcggc gtgatcatgg acgtcgtcac gtcggagcag gccaagatcg
     3541 ccgaggaagc cggcgccgtc gcggtgatgg cgctcgagcg cgtcccggcc gacatccgcg
     3601 cacagggcgg ggtgtcccgg atgagcgacc ccgacatgat cgacggcatc atcgacgccg
     3661 tgtcgatccc ggtgatggcc aaggcgcgca tcggccactt cgccgaggcg caggttctgc
     3721 agagcctcgg cgtcgactac atcgacgagt cggaggtgct gacgccggcc gactacgcca
     3781 accacatcga caagtggcag ttcacggtgc ccttcgtctg cggcgccacc aaccttgggg
     3841 aggcgttgcg ccggatcacc gagggcgcgg cgatgatccg atccaagggc gaggccggca
     3901 ccggggacgt ctccaacgcc gtcactcaca tgcggaccat ccgtgcggag atgcggcgcc
     3961 tgcagtcgat gagcgaggac gagctgttcg tcgcggcgaa ggagctccag gcgtcgtacg
     4021 acctcgtccg cgaggtggcc acgctcggca ggcttcccgt ggtgctgttc acggccggcg
     4081 gcatcgccac gccggcggat gcggcgatga tgatgcagct cggcgcggag ggtgtgttcg
     4141 tcggttccgg catcttcaag tccggcaacc cggcgcagcg tgccgccgcg atcgtcaagg
     4201 ccacgacgtt ctacgacgac ccggacgtgg tcgcgaaggt ctcgcgtggt ctcggtgagg
     4261 ccatggtcgg catcaacgtc gacgagatcc cgcagccgca ccggctcgcc gagcgcggct
     4321 ggtaggcgta cgtcgtcgat ggcgatcggg agtctcgaca aggccgggcg gctcgagcag
     4381 ctgaagacgt acgcccgtgt ccaggtctgg tccgggtacc ggagtcctgc ggacgtgcgc
     4441 tccgatgtgt atgacgccct gctcgacgag gtgaaggacc cctccgaagc ggaacggctg
     4501 acgtccgact acctggtgca agccgaacga gacctcgagt ccgcagcggc cgagtggccc
     4561 gacccgtccg ccttcgaccg cctgcaagcc acgttcgagg acctgcgcga gcagggtctc
     4621 atcgtgctcg aggcgtgtga ggaccactgg tcggcgaacg ccgccctgga gacggccggc
     4681 gcagagggtc ccgagccggc cgggatcgcc tacttcacgc acgccgacgt ctggcatgcc
     4741 gtcgagcacg gcatgctcga gctcaacgtc tggcacccca gcacggcgaa cgtcgcgccg
     4801 ggggatccgc tcctggaacg tgtgcaggaa gcgcttgccc gccaccgcat ctcgtcggtc
     4861 ttcgacgagg gccgcatcga ggtctcggtg gcctgggaac ggcgccccgc acgccctcac
     4921 tctgcgtgac gtgcctgcgc gcggccgccg gatgcccgcc aaggagggcg agccgcctga
     4981 catgccgcga cgtccaccgg tgaccagtcg cccatagccc gttcgggcca tgaagggcgg
     5041 ggcgcgtcgc tgcttacgca gcgtcatgtg gtcagaagcc gggcctccaa aggtgcggaa
     5101 gcgaaatcga agggacaaaa ccgcgtcacg aactcccagc gggatcgttc ctgttccgga
     5161 cgcggtcacc gatgacccgt ccgcctccga ctcgaaaggc ccgctaccct catgaagaag
     5221 ctcacccgtc gtaccgccgc cgtcggcgtc gtcaccctcg gcctcgtcgg cgccggcgtc
     5281 gccttcgccg cgtggaccag caccggcacc ggcagcggct ccgctgaggc gacctctgcc
     5341 gtcgacctcc aggtgcacgt ggaccccgcc accgggctgt accccaccgg cagcaagaac
     5401 gccaccttca cggtcacgaa ccagaacccg tacgccgtca ggctgatcac cgctgaggcg
     5461 gcaaccgact tctccgtcga cgccgctcac agcacctgca acctggactc gctgcacagc
     5521 gcagcgcaga tgctcaacga cgtcatcccg tccggtcaga ccagcgctcc gcacacggtc
     5581 gcgctcagca tggacaacaa cgccaacgac gcctgccagg gtgcgacctt cggcttcaag
     5641 ctgacggtcc acggcgcgag cagcagctga ccagccggct tcacgaaact gaaccatcgc
     5701 acgacgacga cgggactgaa ctgacgtgac cgagcgcagg cgtacgcgcc gcaccctcgt
     5761 gttgctgctc gccctcatcg ggctgacagc gacgggggcg acgcacgcct cgctcgggcc
     5821 gtcggagccg gacccgtcgg tcttcgtcgg caatggtgtg ggcgaggcga tgccacgcgg
     5881 cgccgccggc acctcgagcc tgcagccggc gacccccacg tcgtacgtcg gagtcaatgg
     5941 tcacggctcc ggccatcgtg gggtcagcgg gcacgactgg tggcgcggcc cgatctcggt
     6001 agcggtcggc actgtcagcg ggctctaccc gggactcacc aagcggctcc ccgtcaccgt
     6061 caccaacccc aactcgttcg ggataggggt gacgcggatc aaggtgcgca cgctcggcac
     6121 ggtgacgtgc agcggccact ccttcacggc tcggcacagc agcctcgtgc gccagcgctt
     6181 catccgagcg acccggacac ggacgttcgc gatcccttac cggatgaggc gcaccgcacc
     6241 cgacggctgt cagcgggcgc acgttgcgat ccgcgtacga gtccgggcgg tgcaccgatg
     6301 aggcgcctct tgatcgtcgc tgccctcgtc gcggtgggcc tggccgtcca gccggcgatg
     6361 gccatggcgg agtggctctc accaggcacg ggctccggca gctcgacggc cacccgcgtc
     6421 aattcgccgg tcgcccccac ggtcgtccgg agcggatcga acgtcgtcgt ctcctggcca
     6481 cagggcaagc tcgcgaacgg cactgtggtc accggctacc aggtgaagcg gaccgtaggc
     6541 acgacaacga cgacgctgtg cacgacgacc gaacccacgc gcacctgcac cgacggcaac
     6601 cccgcaggca ccgcttccta cgccgtcgtc aaccggtatg cgaactggag tgcgactggc
     6661 ccgaccacgt ccctcacgtt cgaccagacc gccccggcga cgacgctcac cagcaacccg
     6721 gcgccgaacg ccgcaggctg gaacaagacc gatccgacgc tgacgctgac ggcgaccgac
     6781 gccggcggct caggggtcgc ttcgatcacc tacaagatcg gcagcgggtc ggccgtgacc
     6841 gtcaacggcg cgagcgcttc cttcccggtg acgacgacgg gcgtcacgac cgtcacctac
     6901 ttcgcaaccg acgtcgccgg caatgtggag gcgaccaaga cctccacggt gcggctcgac
     6961 aagacgagcc cgaccttgac caacgtcttc cccgcgaaca acggctccta cacgaaccag
     7021 cagtacagga tcggttgcac cccgcagcgc tccgtctgtg gcaacgcgaa cgacaaccag
     7081 caggtggtca ccgtccagat caagctgatg aactcgtccg gtcagtgcct cggcagcggt
     7141 ggcggcttca cgacggccaa ctgctcggcc tacctgaccg caaccgggac gaccacgtgg
     7201 tactccacca ccgggaacct cgcgaagagc acctacacgt tgacggtgct ggtgaccgac
     7261 gtggccggga acacgaacgg cccggcagga cagaccttcg ccttcacgcg gagctgacgc
     7321 tcggtccctg accgacacca gagcattcgg ccgtggcaac ctacgatggt cgggtgcccc
     7381 gtacatcttc cccgacgatc ggtgtgctcg cgctgcaggg cgatgtgcgc gaacacctgc
     7441 gcatgctgag cggggtcggc gccacgcctg tcgccgtgcg acgtccctcg gagctcgccg
     7501 cggtggacgc gatcgtgctg ccggggggcg agtcgacgac gatgcacaag ctcgcgcgtg
     7561 tcttcgggct gttcgagccg ctgcagcgtc ggctgtccga cggcatgccg gcgctcggca
     7621 cctgtgccgg aatgatcctc cttgccgatc ggatcgagtc aggaatcgat ggtcaggaga
     7681 cgctcggtgg gctcgatgtc gtcgtacggc ggaatgcgtt cggccggcag gtggactcgt
     7741 tcgaggtcga cgtggacttc gagccgttcg accggccgtt ccatgccgtc ctcatcaggg
     7801 cgccgtgggt cgagaaggtg ggagacggcg tcgacgtcct cgcgaccgtc gccggcggcc
     7861 ccgccgccgg tagggtcgtg gccgtccgcc aggggaacat catggccacg tcgttccacc
     7921 ccgagatcac ctcagacgac cgggtccacc actacttcgc cgatctcgtc cgcaattccc
     7981 gaggagctca gtaggcatgt ccggccactc gaaatgggcg accaccaagc acaagaaggc
     8041 ggtcatcgac gcccggcgcg gcaagctgtt cgccaagctg atcaagaaca tcgaagtggc
     8101 ggcgcggacc gggggtggtg acccggcggg gaaccccacg ctgtacgacg ccatccagaa
     8161 ggcgaagaag tcctcggtgc cgaacgacaa catcgaccgt gccgtcaaac gtggttcggg
     8221 actcgaagcc ggtgccgccg actaccagac gatcatgtac gagggctacg cgcccggcgg
     8281 tgtcgccctg ctgatcgagt gcctcaccga caaccggaac cgggcggccg gtgaggtccg
     8341 gaccgcgatg actcgcggcg gcggatcgat ggccgatgcc ggttcggtgt cgtacatgtt
     8401 cgcccgcaag ggcgtcgtca tcgtgccgaa ggccgtggac aaccaagagg gcggtgcgac
     8461 cgagctcgac gaggacaccg tgctcgccgc cgttctcgac gcgggcgccg aggaggtcaa
     8521 cgacctcggt gagtccttcg aggtggtctc ggagccgacc gacctggtgg ccgtacggac
     8581 gtcgctgcag gacgccgggc tcgactacga gtcggccgag gcggcgttcg tcccgagcat
     8641 caccgtcgac gtcgacaagg atgtcgcggg tcgggtgttc cggctcatcg agacgctcga
     8701 ggactccgac gacgtacaga acgtcttcag caacgtcgac gtctccgacg acatcctcgc
     8761 gcagctcgcc tgacgggctt gcgtccgtac ttcctgccgc cccgccgacc ggcgctacgg
     8821 acgcaagcag acgcgagcag gcgcaggcag acgcgagcag aacggcggcg cgtcggtgcc
     8881 gggttgtgtc gctgccggcc gttacgttgg tggcggtcga acatctgttc ggacctggac
     8941 tgacgtcgcc gtctcgtgag gagcatgccg tgcgggtgct gggcgtcgac ccggggctca
     9001 cccggtgcgg gctgggcgtc gtcgagggcg gattctcgca gccactgcgg gcgatcgccg
     9061 tcgacgtggt ccgtacgccg tccgatgccg acctcggagc ccgcctggtc gcgatcgagc
     9121 gggaggtcga ggcctggctc gacgcctacg cgcccgatgc cgtcgccgtg gagcgtgtct
     9181 tctcccagca caacgtgcgc accgtgatgg gcacggcaca ggcgtccgga gtcgccgtcg
     9241 tggctgcggc ccggcgcggc ctgccgatcg cgctccacac gcccagtgaa gtcaaggcgg
     9301 cggtgaccgg aagcggacgc gccggcaagg agcaggtcac cgcgatggtg acccgggtgc
     9361 tgcggctcga gtccagaccg accccggccg acgccgctga cgccctggcg ttggcgatgt
     9421 gccacatctg gcggggtcag gcgggaggcc ggctcgacga agcgctcgcg aaggcggcgg
     9481 gcggatcgag gagtggagat cgatgatcgc gttcgtcaag gggaccgtgt cggccgtctt
     9541 cgtcgactcg gccgtggtcg acgtcggcgg cgtcggcgtc ctcgtgatgt gcacgccggc
     9601 ggcgttggcc acgctccggg tgggcgacga ggccacgctc gcgacctcca tggtggtccg
     9661 ggaagactcg ctgacgctgt tcggcttcgc cgactacgac gagcgcagcc tgttcgagct
     9721 gctgcagact gccagtggcg tcgggccgaa gctggcgcag gcgatgctcg cggtgcacgg
     9781 tcccgacgag ctgcgacgcg ccgtcatgac cgaagacctc gtcgccctct gcaaggtgcc
     9841 cgggatcggc cgcaagggcg cgcagcggat cgtcctggag ctcaaggacc gggtcggcgc
     9901 cccggcgggc ggcgccgtac ccacgcacgc tcccgcgagg gttgccgcgg acggctggcg
     9961 tgaccaggtc cgcgcgggcc tgctgggact gggctggtcg gtcaaggagg ccgaggccgc
    10021 tgtcgacgcc gtcgccccga tggctgccga agcactcagc gacggcgaaa ctgcccctgt
    10081 tgccgagctg ctgcgggccg cactccagtc gctgtcgagg gcatgacgga ggggtggtga
    10141 gagacatcga ggagcggtac gcgttgccac cactggacga acctgccggt cgcggactcg
    10201 tcaccgcgga cgccgacggc gacgagcgaa cgctggaggc tgcgctgcgc ccgcgcacac
    10261 tcgccgagat catcgggcaa gagcgtgtcc gcgagcagct cggtctggtg ctgtccgcgg
    10321 cccgtggtcg ggggcgctgt cctgaccacg tgctgttgtc gggaccaccc gggctcggca
    10381 agacgacgat ggcgatgatc atcgccaccg agatgggtgc gccgctgcgc gtcacgagcg
    10441 gaccggcgat ccagcacgcc ggcgacctgg cggcgattct ctcgggtctc ggcgagggcg
    10501 aggtgctgtt cctcgacgag atccaccgga tgtcccggcc tgccgaggag atgctctaca
    10561 tggcgatgga ggatttccgc gtcgatgttg tcgtcggcaa aggtccgggc gcgacggcga
    10621 ttccgctgga gatccccgtg ttcacgttgg tgggtgccac gacacgcgcc ggactgttgc
    10681 ccggcccgct gcgggaccgg ttcgggttca ccggtcacct cgacttctac gacgtcgacg
    10741 agctgcagca gatcgtcaac cggtcggctc gactgctcga ggtcgaggtg gacgccggcg
    10801 gagcgaacga gatcgcgtcc cgctcccgcg gcaccccgcg gatcgccaac cggttgctgc
    10861 ggcgggtgcg cgacttcgcc caggtgcgtt ccgacggacg cgtcagccgt gaccttgctc
    10921 acgacgcgct ggccctgttc gaggtcgacg agtccgggct ggaccgcctc gaccgcgcgg
    10981 tgctcgacgc actgtgccga cgcttcggtg gtggcccggt cgggctcggg acgctggccg
    11041 ttgccgtcgg ggaggagcgc gagaccgtcg aggaggtggc cgagccgttc ctggtccggt
    11101 ccggttacct tgcgcgtacg ccgcgcggcc gggtcgccac accggccgcc tggcaccacc
    11161 tcgggatgac cgccccccgt gccgctccgt atcaggacca ggaggcgctg ttcggcgatc
    11221 gctgaggggc ccgttcggtc atcaatcggt caccaaacgg tcactgccgg ccactccgat
    11281 tcaagatttc cggacatcgc cggttagact ccgcctttgg ccgtgcaccc ggcccctcga
    11341 tcgtgttccc gagctgatcc tgacgtgccc gcccggccgg ggatcgcaac aaggagtcaa
    11401 ccaggtgagt ggtaacgccc tcgctcagct gctgccgctt ctcctgctgg tggtgctggt
    11461 ctacttcctc atgatccgtc cggcccgtcg ccgggctcag caggtctccc gtctccaggc
    11521 cgccttgtca gtcggcgacg aggtgatgct gagcagcggc atcttcggca cggtcgaatc
    11581 ggtcttcgac gaccgcgtga acgtcaaggt ggccgacggc gttgtactgg ttgtgcatcg
    11641 tggcgcggtc aacgagatcg tccgcgacgt accggcggag tccgccaccg cggccgatgg
    11701 tgagcccggc ggtgcggagg accagcgcct acccgagcag gacccggacg accccggcac
    11761 ccacgaagac acgacccgag gagcgaactg acgtggcggc caagacgtcg catccgaccc
    11821 ggacgctcct gatcttcatg ctcgtcatcg gcggcctgta cggcgctgcc gcggcggtcg
    11881 gcacctggaa gccgaagctc ggcctcgacc tccagggcgg cactcgcatc accctgcagg
    11941 ccaagtccac cggaggcggt tccgtcacca gcgacaagct cgacgaggcc gtcgacatca
    12001 tcagcaaccg ggtgaacggc gccggcgtcg ccgagtcgga ggtgtcgacc cagggcagcg
    12061 acatcatcgt ggtcgagatc ccgggcaagg tcgacaagaa cctcgagcgc acgatcgggt
    12121 cgacagcaca gctgcgattc cgcctcgagg cgctacgact gccgcctgcc ggtacgacgc
    12181 tgccctcacc gaccgacacc ggcgccacga gtcccagcgg cagcgagccg acgagcccga
    12241 agagcacgac ggggaatgag agcccggcca caccgtcggg tacgaggacc gggccgacga
    12301 tcaagccgaa gccgggcgac aagacgccga acaccacctc ggacaccggc aagaaccgtg
    12361 ccggcgccgc ctggatgcgc agcggcaaca atgcgccgtc gacgccgacc gagccgacgc
    12421 cgagcggtcc cacgacggga cccccgaccg agctcgtgcc gcccgacctg gaggacatca
    12481 aggacccgta cgcctgggtg gcgaacccgc cgcaggagtg gcaggcgcgg gccgccgcgt
    12541 acgtctgccc gggtcccggt cagccgtcga gcgggcagat cgacctgccg agccagccgc
    12601 tcatcgcgtg tgacgaggcc ggcaaccgct tcctgctcgg tccggcgatg gtggagggga
    12661 cacacgtcaa ggacgccagc tacggcatcc cgcagcaggg catcgactac gtcgtcaacg
    12721 tgtccttcga cggtgagggc tccaagcagt tcgccgacgt gaccacgcgg atcgccgggc
    12781 agaccaaccc gctcaacggc gacaagcagc ggctcgcgat cgtcctcgac ggcgaggtcc
    12841 tgtccgaccc gtcgaacgat cagccgatca ccggcgggcg agccgagatc tccggcaact
    12901 tcgaccagga tcaggcgaag agcctggcga actcgctcaa gtacggcgca ctgccactga
    12961 cgttcgtcgt ccagagccgg accgacgagg gtcctgagct cgccagcaac cagctcaccg
    13021 ccgggatcat cgccggcatc atcggactga tcctcgtcgt gatctactgc ctgatctact
    13081 accgcggtct gggcttcgtc gtgatcgcct cgctgatcat cgccgggctg ctgacgtacg
    13141 catgcgtcct gctgttgagc catgcgtacg ggttcacact caccctgccg ggcatcgccg
    13201 ggttgatcgt cgcgatcggc atcaccgccg actcgttcat cgtctacttc gaacggttgc
    13261 gcgacgaggt acgcgaaggc aagacgttgc gcacgtcggt cgagaccggc tggagacggg
    13321 ccaggacgac gatcctggcc gcggatggcg tctcgatcct ggcggcgctg gtgctgttca
    13381 tcttcgcgat cggggtcgtg aagggcttcg cgttcgcgct cggtctgacg acggtcgtcg
    13441 acatcttcgt ggtgttcttc ttcaccaagc cgatggtgac gatgctgagc aggacgaagt
    13501 tcttcggcca cggccacaag tggtccggcc tcgaccgcca gcacctcggc atgccgccgc
    13561 tgaagaagac tccgcgcacg gcgggagggg aggcctgatg tcgaagttcg gacggatcgg
    13621 ggccgccctc taccgcggag acgtctcgat cgacttcgtc ggacgtcgca ggacctggta
    13681 caagatctcg ctggcgattc tggcgatcgc tgtggtcgca ctggtgtggc gcgggctgaa
    13741 cttcgggatc gagttccgcg gcggcgtcga gttccaggcg cagatctcca acccgtcctc
    13801 gaaggtcgag tccgtcacga acgccgtcgt cgacaccggc atcgaggcgg cgcagagtcc
    13861 tgtcgtcgtg gcgtcgaaca acgactcgat acgggtccag accgagccgc tcacgctgga
    13921 cgagatcaac gtcgtctcca acgcgctgac cgacgagggc gccaccgaag tgagcaccgc
    13981 gtcgatcgga cccacctggg gtcaggcgat cgcgaagaag gcgctcaccg gtctcatcgt
    14041 gttcgtcatc ctcgtggtcc tcttcatctg ggcgtacttc cgagaatggc ggatgtccgt
    14101 cgcggcgatc gtggcgctgg cgcacgacct gctgatcacg gtcggcatct acgccctgac
    14161 cggcttcgag gtgacacccg ccacggtcac cggcttgctg acgatcctcg gttactcgct
    14221 gtacgacacg gtggtggtgt tcgacaaagt tcgcgagaac acccgaaaca tcacggcgtc
    14281 gacgcggcgt acgtatgccg agagcgccaa cctggcggtc aaccagaccc tggtccggtc
    14341 gatcaacacg tcgatcgccg ccttgctgcc cgtcggagcg atcttgtacg tcggcgcgtt
    14401 catgctcggt tcgggcccgt tgaaggacct ggcgcttgcg ctgttcgtcg gcatggccgc
    14461 cggggcgtac tcgtcgatct tcatcgcgac acccttgctc gcccagctga agatgcgcga
    14521 gccggcgatg caggcgcagg ccaagcgggt gacgtcccgt cggacggctg cggaccggat
    14581 gatcgtgcac gacccggaac gggctgctgc cgaggccgag agtgcggagc aggaggacga
    14641 cgggaagccg cccgctccgc gacccgtcgt gacgcggacg gccaccaaga cggcgccgcg
    14701 tccgacgagc agctcggcca gcaagcggtc gcagccgcag cgtcaggccc gctccaagcg
    14761 cggcaagcga tgagtgccgg ggcgcctgac gacctcgcgg cactgatcgc gcccctcgta
    14821 cgcgatgtct cggatttccc ggagccgggc atcgtcttca aggacctcgg tccgctcttc
    14881 gccgaccacg acgcgttgac gcgggtcgtg gaggcgatcg ccgacgcggg ccgcgcggac
    14941 ggccgcgggc gggtggacaa ggtggtcggg atcgagtcgc gcgggttcat cctcggtgca
    15001 ccggtcgccc tggagctggg cgccggcttc gtgccggtgc gtaagcaggg caagctgccc
    15061 ggcccgacgt tccaacggtc gtacgcgctg gagtacggcg aagccactct cgaggtccag
    15121 cgggatgcct tcgcgccagg tgacttcgtg ctggtcgtcg acgacgtact ggcaaccggc
    15181 ggcactgtgg aagcaacggc tgctctggtc gagcagtgtg gcgccgaggt cgtcggcatc
    15241 gcggtcctga tcgagctcgg tttcctttcc gggcgcgacc gcgccgccgg tcatgccgtg
    15301 acctcgctgt tgatcctgta gtcccgcccg agacaacggc gacttgcgat agacgccggc
    15361 ccgcgtcagc cgaggtggcg gttttcgatc caccctgcga tgtgtgctgg acgaatccgc
    15421 catgtcggga ccgtagtgaa ggtgtgctcc ccgtcgaggt ccagcccgag ccaacggcga
    15481 cttgcggtta gcgcggccct cgcatcgctc accagtggtt ccgtagactg gttgttcgga
    15541 gacggagcag aaggtcagga gcacggcgtg gcggacgagg ttctcgctac cacgacggac
    15601 gagccggcag ccggcgagag cgctgccgca cccaccagga tgcggacgag gctggcccgg
    15661 atcggtcggt cgagcgggtt cgaccctgtc ctggagccgc tgttccaggt ggtccgcaac
    15721 acccacccga aggccgatct cgcgatcatc gagaaggcct acaaggccgc cgagcggcat
    15781 catcacgggc agacccgccg tagcggtgac ccgttcatca cccatccgct ggcggtgacg
    15841 acgatcctgg ccgagctggg catgacgccg ccgaccctgt gcgcggcgtt gctgcacgac
    15901 acggtggagg acacgccgta caccctcgac cagctccgcg ccgacttcag tgacgagatc
    15961 gcgctcctcg tcgacggcgt caccaagctg gacaaggtca gctacggcga ctcggcccag
    16021 gcggagacga tccgcaagat gatcgtcgcg atgagcaagg acatccgggt cctggtcatc
    16081 aagctcgccg accggttgca caacatgcgc acgctgcggt tcctgcgtca ggccacgcag
    16141 gagcgcatcg ccagcgagac gctggagatc tacgcaccgc tggcccaccg gctcggcatg
    16201 aacacgatca agtgggagct ggaggacctc gcgttcgcga cgctgcaccc gaaggtgtac
    16261 gacgagatcg tccggctggt cgcagagcgg gcccccgcgc gcgacgagtt cctcgcgacc
    16321 gtcatcgacc aggtgcagca cgacctgcgt ggagccaaga tcaaggccac ggtgactgga
    16381 cgccccaagc actactactc gatctaccag aagatgatcg tgcgcggccg cgagttcacc
    16441 gacatctacg acctcgtcgg gatccgcatc ctcaccgact cgttgcgcga ctgctacgcg
    16501 gtgctcggcg tcttgcatgc tcggtggaac ccggtgccgg gacgattcaa ggacttcgtc
    16561 gcgatgccga agttcaacat gtaccagtcg ttgcacacga ccgtgatcgg tccggcaggc
    16621 aagccggtcg agctgcagat ccgcacccag gcgatgcacc gccgggcgga gtacggcgtc
    16681 gcagcgcact ggaagtacaa ggaggacggc cgcgccggcc tcgacaccaa ccggccgctg
    16741 aacgccgacg aggggtcggg ggacatgatc tgggtccgcc agctgttgga ctggcagcgt
    16801 gagaccgagg acccggggga gttcctcgac tcgctgcgct tcgagatcaa ctctgccgag
    16861 gtctacgtct tcacgcctcg tggggaggtc atcgcgttgc cgaccggttc gacgcccgtc
    16921 gacttcgcgt actccatcca caccgaggtc ggacaccgct gcatcggtgc gcgcgtcaac
    16981 ggtcgcctcg tgccgttgga gtcccagctc gacaacggcg acgtggtcga gatcttcacg
    17041 tccaaggcgc agaacgcggg acccagccgg gactggctga cgttcgtcaa gagcccgcgg
    17101 gcgcgcaaca agatcaagca gttcttcacc aaggagcgtc gcgaggaggc gatcgagcgg
    17161 ggcaaggaga cgatcgctcg gctgatgcga cgcgagggtc ttccgctgca gcggatgctc
    17221 acccacgaca cgttgtccgt cgtcgccggc gagctgcggc tgccggacgt gaccgcgttg
    17281 tatgccgcgg tcggcgaagg gaacacgacc gcgcagaccg tcgtacgccg gatcctcgag
    17341 ctcttcggtg gcgacgacgc ggcagcggac gaggcggccg agagcacggt cacgatcacg
    17401 agcgagcggg agcggcgcaa gcggtctccg cgtggcgatg cgggtgtgat cgtcaagggt
    17461 ctgagcgatg tctgggtgaa gctggcgcgc tgctgcaccc cggtgccggg cgacgacatc
    17521 gtcggcttcg tgacgcgtgg gtcaggcgtg tccgtgcacc gtggcgactg cacgaacatc
    17581 aagagcctct cgtcgcagcc gggtcggatg gtcgaggtcg agtgggcgcc gactgcgcag
    17641 tcgatgttcc tggtggcgat ccaggtcgag gcgctggacc gcaaccggct gctgtcggac
    17701 atcactcggg tgctgtccga cgtacacgtc aacatcttgt cggcgtcggt ggcgacgacc
    17761 cgggaccgga tcgcgaagag ccgcttcacg ttcgagatgg ccgacccgac ccacctcggt
    17821 cacgtcctca aagccgtccg caccgtcgac ggcgtcttcg acgcctaccg cgtcacccag
    17881 tgaccctttt cgttgagtgc gggtttcttg cgtgttgagt gctggattct ggtgtgttga
    17941 gtgttgccgt gtcgggtgtt gagtgctggg ttctggtctg ttgagtgttg ccttgctgag
    18001 gcttgcgagt gctggcgagc ctgcttgtga agtagcccga cgctaaggac gccggaatcc
    18061 aacattcgcc gaccggaaac ccggcactca aagcccggaa acccggcact caacacacca
    18121 gaacccggca ctcaacgaaa acgtcaggtg gagaattcgg tgagggcgcg tttggcttcg
    18181 tcgagccagg actggcgggc ggtgatcgca gccttggccg actcgacctg acgcgcgtcg
    18241 ccggacgcct ccgccgttgc caggtcggac tgcagcgacg cgatcgacgc ctccagctgc
    18301 gccaccgtgt cggccgcccg cgcgtgcgcc tccgggttgc gccggcgcca ctggtcgtcc
    18361 tcggcgccgc gcaccgcctg ctcgactcgc ttgaaacgcg tctccaggtc cttcatctcc
    18421 gtcctgggca ccttgccggc cgcgtcccac ttctcggcca gctcgcggaa ctgctcccgc
    18481 gccgtcctcg ggtcgcgtac gggcaacaag gcctccgctt cgacaagcag gccacgcttc
    18541 acatcggcgt tggcggcgta ctgctggtcc agtgccgcat tggcggcgtc gcgggctccg
    18601 aagaacgcgt cctgtgctgc tcggaaccgt ttccacagcg cgtcatccac gtctttctgc
    18661 gccggcccgg cagccttcca tcgcgacatc aggtcgcggt aggcccgcgc agtcgcgccc
    18721 cagtccgtcg agtctgcgag ctcctcggct tctgcgacga gcttctcctt gacgaccttc
    18781 gcgctgtcac ggcgttcgtt cagctctgcg aagtgctgct tgcgacgccg cgtgtacgcc
    18841 gttcgggccg acgagaaccg atgccacagc tcgtcgtcgc tggccttgtc gatgcgcggc
    18901 aggctcttcc aggtggtgag cagctcacgc agccggttcg caccatgccg ccaatccgtt
    18961 cccgaggcga gtcgctctgc ctcttcggcg atgcgccgct tggcctgctt cgcctcgtcg
    19021 gcccgagccg cacgctccgc ccgccgggcc tcccggcgtt cggcgatcac cggctcgagc
    19081 gcgtcgagcc gagcggccaa cgcgtcgaga tcgccgaccg cctgggcgct ctggatggag
    19141 gccttcacct tcgcgacggt cgcggtcgcg tcctcgggcg agagtgaccc ggccttgatc
    19201 cgctgttcga gcaggtcgac ttcgacgacg aggccttcgt agcgctttcg atagaagtcc
    19261 agagccgcct gcgggtctcc gtcgggccac tggccgacga cccgttcacc gtccttggtc
    19321 ttgacgtaga cagtgccgtc ggcatcgacg cggccgaagc cgccgccgtc gggtgtctcg
    19381 gtcatcggtt ccccctggtg gccgtggcct tgctcgacgg cttgttgtcc gtcggcttgt
    19441 ggttcgactg ggtcggcgtg ttggtggcgc cgccggtcgg ctgcgtgccg ttcgtctgcg
    19501 gtggggtcga gggtgtcagc aggtcggtct tgaacgtgtc ggtcgggacg gccggtgcct
    19561 gcgggctgcg ggtgttctcc tcgtcgatga tgtggacgac gagccacgcg agaagcgcgg
    19621 ccgcgacgag accgatgacg acaccgacga cgatgctgat ggtccgctgt cgccgagagt
    19681 cgcgcacccg tcgcgtctgc tggcgttccc actttgcacg cgccaactga cgttgacgct
    19741 gcttgctcgt aaccacacgt acctccgtga ccccggaacg gtggaacagt ctaagaggga
    19801 gtccgaaacg ccaacgcacc ccgtgccgat cgtgaccatc gcgttgtttt cagccgccct
    19861 gctccccgtc cgacgtcgcc accggggcga gggacgggcg ttcggccgcc aggttgacgc
    19921 tcggtaggct tccgggaccg tacgaaacga gaggcccgct gtgctcatcg ccgccttccc
    19981 cgccggtccc tgggcgacga actgttatgt cgtcgcgccc ggtccgcgcg aggagtgcgt
    20041 ggtgatcgac cctgggtatg acgctgcgtc cggcgtcgcc gcggtcgtgc gcgagcacca
    20101 cctacgccca gccgcggtcc tgctgaccca tggacatgtc gaccacatct ggtcggtgac
    20161 gccggtcgcc ggtgcctacg acgcgacggc gtacatccac cccgccgacc ggcacttgct
    20221 caccaacccg gccgccggac tctc
//
LOCUS       DATBFZ010000059         6829 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_4615100, whole genome shotgun sequence.
ACCESSION   DATBFZ010000059 DATBFZ010000000
VERSION     DATBFZ010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 6829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6829
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4615100"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1346)
                     /locus_tag="VH419_01805"
     CDS             complement(<1..1346)
                     /locus_tag="VH419_01805"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY7042381.1"
                     /translation="MGEEGPPAGQPSGSGVDGPDRDDASVGGIHAFLIADMRGYTAYT
                     DRYGDEAAARLAERFAILATEVISRHHGHVLELRGDEALAVFTSPRHALRASVALQAA
                     LADAPDAEDGLPVGIGLDAGEAVPVQGGFRGAPLNRAARLCSLATGGQVLATPEIVHL
                     AGQLQDIEAVPSGRVTLKGFQQPVDVLSIGPGDPTTREPASRSGMVGLLARFGLGRRR
                     TRVVLGTVAATVLVLTGATIIPSLIPGSAQSSGRDTPPPSPADPPDLLATLIFVPDFS
                     PRSAPAVFMIPDSQGPPPALPKHHLDGPPPIASVGDDAYNRWHVNHGGIPENSQTVRL
                     VLGARTDQPVIITKVTPYVVTRKPPVHGWNFNPETGAGVPLRFVQASLDCPEHAAWLV
                     TTDPKTMVVVDRTTAIDLKVSRRDAEELEVTAFSTESYVQWGLEITYVSHGKLKTLR"
     gene            1603..4533
                     /locus_tag="VH419_01810"
     CDS             1603..4533
                     /locus_tag="VH419_01810"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY7042382.1"
                     /translation="MHQPTAAELPLVGRARELASLQQAIDDARHARTTVVHLIGGAGL
                     GKSRLVLEACRMAEASGATVLSGAASDPAGLTPFATVRSPFETLSDIRGDHGLGRHLG
                     LAGDDLAVVLEPHEPSNVEFSVRRWREVLRRAMTNMAARGPVLVAFDDVHWADEPSRD
                     VLASLARRPPPAGFVLVLAYRWRQELGPLLAASSADVASTPGFSVRRLELSALDADDI
                     AELLPHAGPARRQSLLQASHGNPFFVHALHRQPMVPGLGFDEAGVIPSAVQAALAGEL
                     DRLDPADRRLVDVAAILGGTFDPHLVADVVGVSVEEALAGLDRSAQEGICAAEHSATT
                     WRFRHPLLRLAAYDAQSAVTRAATHRRVQQVLAAHGAPAAEQARHVAVTARSGDQDAA
                     HLLVTAAGEVADRSPAMAERWLTTALQLTADGGLDDVRDIRLARCRATTALGRLQDAR
                     AEAHEVVSTLGHDDSRRVDAVRFAAHLDQLLGSHAESDALLTRELATRAGTRQDDPDG
                     IALLVDLATTRLMRGDFERARVPAEQALTLVADLDPTRAPGLSAATLSLHALVAHADG
                     DRAHAVADRESASQSVDALTDVELAGHLEAGLWLGWVEMFLEEYAAGIRHLSRCLDIA
                     RRGTHQHLLTHLLVGYGSLLKLRGDLPGAAEAYDEAAEVAHRTQSTELLVMAETMQCR
                     AATWLGDHDRARRLGERAVASTAEQGSWFEAIARAVLAQARLVGGDASGVLDAVISAG
                     GGPELPRFDPASRCDWWEVATRAAIAEGDLVTAQGLVERVSETSRTLELRAPRGAAAL
                     ARAQLSAALGHWHVAVEEAHAAAAQFAAVGNRLERGRALLVSGSALAEAGQRKAALEE
                     IQTAEHDFATCSAAHFQALSRSLMRRLGRRVPSPRRTPVHETVGPASAPTSTNDALAT
                     LSRREWEVARLVSAGHTNRQIASELVVSEKTVESHVSHIFTKLGVASRTAIASAVAAT
                     GAGS"
     gene            4711..4935
                     /locus_tag="VH419_01815"
     CDS             4711..4935
                     /locus_tag="VH419_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042383.1"
                     /translation="MTIDRELELTSCPECGELAEIVWRAVLASSDGPIGHAYVRCVRR
                     HWFLMPSEGLSAPDPSSASATSRHIKEEHV"
     gene            4932..5552
                     /locus_tag="VH419_01820"
     CDS             4932..5552
                     /locus_tag="VH419_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042384.1"
                     /translation="MKIKKRTAAVLSAAGLAAAALPLTMAGHGEAAPDATHFRMIRSS
                     GVVAAGCLEHAGARVTVTPKGPVEVMDIKAWGLPKRREFDLFVTQQPDAPFGMSWYQG
                     DMESDKYGRASGHFVGRFNVETFIIAPGSVAAPTPHDELDADTNPATPPVHTFHLGLW
                     FGSPRAAHAAGCPTTVTPFNGEHHAGIQALSTRQFAATEGPLGNIE"
     gene            5737..6456
                     /locus_tag="VH419_01825"
     CDS             5737..6456
                     /locus_tag="VH419_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020386402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY7042385.1"
                     /translation="MAAMVEGRRGSDGGGLRVVFADDNFLVREGVAALLAEVEEVDLV
                     DTTSEPNALLRSVAEHRPDAVLTDIRMPPTFTTEGIEAAKRIRGEFPDTGVVVLSQYV
                     EEDYAFELLADGVEGLGYLLKERVSQVDELVRALQDVAAGGSALDPKVIEGLLSRKTR
                     EARSPLSQLTERELEVLQLMATGRTNAVLAKELFMSERAVEKHVSAVFQKLGLADERE
                     TNRRVMAVLTYLEATGGASSR"
     gene            6563..>6829
                     /locus_tag="VH419_01830"
     CDS             6563..>6829
                     /locus_tag="VH419_01830"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY7042386.1"
                     /translation="MADVRVLVVDDQEPFRRAAAGVIDEMAGFVVVGSVESAERSITA
                     VAELHPDLVLMDVNLPAMNGIEAARRLTSANAGLVVLLLSTYDED"
BASE COUNT         1067 a         2285 c         2415 g         1062 t
ORIGIN      
        1 cgcaacgtct tgagcttccc gtgcgagacg taggtgatct cgagacccca ttgcacgtag
       61 gactcggtcg agaatgcggt cacctcgagt tcctcggcgt cgcggcggct gaccttcagg
      121 tcgatcgcgg tggtgcgatc cacgacgacc atcgtcttgg ggtcggtggt gaccagccag
      181 gccgcgtgct caggacagtc gaggctggcc tggacgaaac gcaacgggac tccggcgccg
      241 gtctccgggt tgaagttcca gccatggacc ggaggcttcc gggtcacgac gtacggtgtg
      301 accttggtga tgatgaccgg ctggtcagtt cgcgcgccaa ggaccagccg gaccgtctgc
      361 gaattctccg gtatgccgcc atggttcaca tgccagcggt tgtaggcatc gtcacccacc
      421 gaagcgatcg gcggtggacc gtcgaggtga tgcttcggaa gtgcgggtgg tggaccttgg
      481 gagtccggaa tcatgaagac ggccggtgcg gatcgtgggc tgaagtccgg cacgaagatc
      541 agcgtggcga gcaggtctgg ggggtccgcg ggactcggcg gcggggtgtc gcgaccgctg
      601 gattgcgccg agcccgggat cagtgacggg atgatcgtcg ccccggtgag caccagcacg
      661 gtcgcggcaa cggttcccaa caccacgcga gtgcgccgac gacccagccc gaaccgagca
      721 aggagtccga ccattccgga tcgcgacgcc ggctcgcgcg tcgtcgggtc gcctgggccg
      781 atcgacaaga cgtccaccgg ctgctggaag cccttgagcg tgacccggcc ggagggcacc
      841 gcctcgatgt cctggagctg cccggccagg tggacgatct ccggtgtcgc cagtacctgg
      901 cctccggtcg cgaggctgca caggcgtgcg gcgcggttca agggagcacc ccggaacccg
      961 ccctgcacag gcaccgcctc gcccgcgtcg agaccgatcc cgacgggcag cccatcctcg
     1021 gcgtcggggg catcagccag agccgcctgc agcgcgaccg acgcacgcag cgcgtggcga
     1081 ggtgacgtga aaacggccag cgcctcgtca ccgcgcagct cgagcacgtg accgtgatgc
     1141 ctgctgatca cctccgtcgc gagaatggcg aacctctcgg caagtcgcgc tgccgcctcg
     1201 tccccgtacc ggtcggtgta cgccgtgtag ccacgcatgt cggcgatgag gaacgcgtgg
     1261 atgccgccga cgctcgcgtc gtcacggtcg ggtccgtcga cgccggaccc ggagggttgc
     1321 ccggctggcg ggccttcctc acccatggcg tgagtgtcgt ctccgacgaa cgtttcgtgc
     1381 aagcacattg cgcgagcgat cggtcgtgtc atccgccgcc gattccaagc tcggagagca
     1441 tgcgggacgg cagacgcggt caggcaggga aggggccgta tcacggacaa accacgcgac
     1501 tcctgcacat cgtcaggcgt agggtcatat caggccgtgg cggttgcgtg ctcgacccct
     1561 cgcggtcgtc gaaggagtcg catcgtcgag gcagtgagac ccatgcacca gccaaccgct
     1621 gcagagttgc cgttggtcgg gcgcgcccgg gagctggcca gcctgcagca ggcgatcgac
     1681 gacgcccgcc atgcgcgaac cacggtcgtc cacctgatcg gtggcgccgg gctggggaag
     1741 agccggcttg tcctggaggc atgccggatg gccgaagcct ccggcgcgac ggtgctgtcg
     1801 ggggcagcca gcgaccctgc cggtctcact ccgttcgcaa cggtgcgcag cccgttcgag
     1861 acgctcagcg acatccgtgg cgatcatggt ctcggtcgac acctgggcct tgccggtgac
     1921 gatctcgccg tggttctcga accacacgaa cccagcaatg tcgagttcag tgtccgacga
     1981 tggcgtgagg tcctgcgccg ggcgatgacc aacatggcgg cgcgcgggcc ggtgctcgtc
     2041 gccttcgacg atgtccactg ggccgatgaa ccctcccgcg acgtcttggc atcgttggcc
     2101 cggcgtccac caccggcagg cttcgtgctc gtgctcgcct accgatggcg gcaggagctg
     2161 gggccgttgc tggcagcgag cagcgcagac gtcgcctcga ccccagggtt ctccgtacgc
     2221 cggctggagc tgtcggctct ggacgccgac gacatcgccg agctgctgcc ccacgcgggt
     2281 cctgcccgca ggcagtcgct gctgcaggcc agccacggca atcccttctt cgtccacgca
     2341 ctgcatcgac agccgatggt gcccggactc ggttttgacg aggcaggcgt catcccaagc
     2401 gccgtccagg cggcactcgc cggagagctc gatcggctcg acccggccga tcgacgtctc
     2461 gtcgatgtcg cggcgatcct gggcggaaca ttcgacccgc atctggtcgc cgacgtcgtc
     2521 ggcgtttcgg tcgaagaggc tctggccggg ctggaccggt ctgcccagga ggggatctgc
     2581 gctgccgagc actcggcgac aacctggcgc ttccgtcatc cgctgctgcg gctggcggcg
     2641 tacgacgccc aatccgccgt cacccgggcg gccacgcatc ggcgcgtcca gcaggtcctc
     2701 gcggcgcatg gtgcgccggc ggccgagcag gcacgccatg tcgcggtcac cgcccgctcc
     2761 ggcgaccagg acgcggccca cctgctcgtc actgcggcag gcgaggttgc ggacaggtcg
     2821 ccggcgatgg ccgagcggtg gctcacgaca gcgctccagc tcaccgcgga cggtggtctc
     2881 gacgacgtcc gggacattcg gttggcgcgg tgccgcgcca ccaccgcact cggcaggctg
     2941 caggatgcgc gtgccgaagc ccatgaggtc gtaagcaccc tcggacacga cgactcccgt
     3001 cgggtcgacg cggtcaggtt cgcggcccac ctggaccagc tgctcggcag ccacgcagag
     3061 tccgatgccc tgctcacacg agagctcgcg acgcgcgccg gcacccggca ggacgaccca
     3121 gacggcatcg cgctgctcgt cgacctggcg acgacgcggt tgatgcgtgg tgacttcgaa
     3181 cgggctcgtg ttcctgcgga gcaggcactc acgttggttg ctgacctcga tcccactcgg
     3241 gcgccgggtc tgtcggccgc aacgctgtcc ttgcatgcac tcgtcgctca tgccgacggt
     3301 gaccgcgctc acgcggtcgc ggaccgcgag tccgcgagcc agtcggtcga tgcgctgacc
     3361 gacgtggagc tcgccggcca tctcgaggcc gggctgtggc tgggctgggt cgagatgttc
     3421 ctggaggagt acgccgcggg catcagacac ctgtcgcgct gcctcgacat tgctcggcgc
     3481 gggacacacc aacacctgct cacccacctg ctcgtcggct acggcagcct gctcaagctg
     3541 cgtggggatc ttcccggcgc cgccgaggcg tacgacgagg cggccgaggt ggcgcaccgg
     3601 acgcaaagca cggagctgct ggtgatggcc gagacgatgc agtgtcgggc ggccacctgg
     3661 ctcggcgacc acgaccgcgc ccgccgactg ggtgagcgcg cagtggcgtc gacagccgaa
     3721 cagggcagct ggttcgaggc gatcgcccgg gcagtcctcg cccaggcccg cctggtcgga
     3781 ggcgacgcga gcggcgtcct cgacgcggtg atctccgccg gcggtgggcc ggaactaccg
     3841 cgcttcgacc cggcgtcgcg ttgtgactgg tgggaggtgg caacccgggc cgccatcgcc
     3901 gagggcgacc tggtgacggc tcagggcctc gtcgagcgcg tgtcggaaac ctcgcggacg
     3961 ctggagctgc gcgcaccgcg tggcgcggcc gccttggctc gtgcacagct gtccgcggca
     4021 ctcgggcact ggcacgtcgc cgtcgaggag gcgcacgcgg cggcggcgca gttcgcggcg
     4081 gtcggcaacc ggctcgagcg cggacgtgcg ctgctggtga gcgggtcggc gctcgccgaa
     4141 gcgggccaac gcaaggcggc actggaggag atccagactg ccgagcacga cttcgccacc
     4201 tgctctgccg ctcacttcca ggcgttgagc cggagcctga tgcgccggct cgggcgacgg
     4261 gtgccgagcc cccgccgaac gccggtccac gagacggtcg ggcctgcatc cgcgccgacg
     4321 tccacgaacg atgcgctggc gacgctgtcg agacgggagt gggaagtggc ccgtctggtg
     4381 agcgcaggtc acaccaaccg tcagatcgcc tccgagctcg tcgtcagcga gaagacggtg
     4441 gagagccacg tctcccacat cttcaccaag ctcggcgtgg cctcgcgaac tgcgatcgcg
     4501 agcgccgtcg ccgcgactgg tgccggctcc tgacagcacc ctgacacgtc cctgacatcc
     4561 gccctgacaa gcgttggctt tgcctgtttt cccaggtcag aaggggtttc tcagatctgc
     4621 cccgatgtca gggattcccc cgatgccgcg ccgtccgaac gcgacgacag tcgactcggc
     4681 ggcacagtgc cgctggaacg gaggggggac atgaccatcg accgtgagct cgagctgacg
     4741 agctgccccg agtgcggaga acttgccgag atcgtctggc gagccgtcct ggcgtcgagc
     4801 gacggcccga tcgggcacgc atacgtgcga tgtgttcgac ggcactggtt cctgatgccc
     4861 tccgaggggt tgtccgcacc cgatccttcg agtgcatcgg caacctcccg acacatcaag
     4921 gaggaacacg tatgaagatc aagaagagga ctgctgctgt gctgtccgcc gccggactgg
     4981 cagcggctgc gctgccgctg acgatggcgg ggcatgggga ggcagcgccg gacgcgaccc
     5041 acttcaggat gatccgctcc agcggcgttg tggctgcggg ctgcctcgag cacgccggtg
     5101 ctcgggtcac cgtcacgccg aagggccccg tcgaggtcat ggacatcaag gcgtgggggc
     5161 ttcccaagcg gcgtgagttc gacctgttcg tgacccagca accggacgcg ccgttcggca
     5221 tgtcttggta ccagggcgac atggagagcg acaagtacgg ccgcgcaagc gggcacttcg
     5281 tcggtcgctt caacgtcgag acgttcatca tcgctccagg ctctgtggcg gctccgacgc
     5341 cgcacgacga gctggacgct gacacgaacc cggcgactcc accggtgcac accttccacc
     5401 tgggcttgtg gttcggttcg ccgcgtgcgg ctcacgcggc cggctgcccg accacggtga
     5461 cgccattcaa cggtgagcac cacgccggca tccaggcact gagcaccagg cagttcgctg
     5521 ccaccgaagg cccgctcggc aacatcgagt gagccaatgg gcgagtgagg ggaatgggcg
     5581 agtgagccct ggccggtagg cagcgtcgac cggccagcac aatgaaagag acctggccgc
     5641 ttccgcggtc cgctgatctc ctgtcgagat cacgggccac cgggggcggc caggtcgtct
     5701 gctgtacgac gagcatccag accctagagt gtcgccatgg ccgcgatggt ggaggggcgc
     5761 cgtggttcgg acgggggcgg tctgcgcgtc gtgttcgctg acgacaactt cctcgtgcgc
     5821 gaaggcgttg ccgcgctgtt ggccgaggtc gaggaggtcg acctggtcga caccacctcg
     5881 gaaccgaatg cgctgctgcg ttcggtcgcc gagcaccggc ccgacgccgt tctcaccgac
     5941 atccggatgc ctccgacctt caccacggaa gggatcgagg cggccaagcg gatccgcggt
     6001 gagttccctg acaccggggt cgtggtgctg tcgcagtacg tcgaggagga ctacgcgttc
     6061 gagctgctgg ccgatggagt cgaaggcctg ggctacctgc tcaaggagcg tgtctcgcag
     6121 gtcgacgaac tggtgcgggc gctgcaggac gtggcggccg gcggatccgc acttgacccg
     6181 aaggtgatcg agggactgct cagccggaag acgagagaag ccaggtcgcc gctgtcccag
     6241 ctgaccgagc gcgagctgga agtcctgcag ctgatggcga ccggcaggac gaacgccgta
     6301 ctcgccaaag agctcttcat gagcgaacgg gcagtggaga agcatgtcag cgccgtgttc
     6361 cagaaactcg ggctggcgga cgagcgggag accaaccgcc gggtgatggc ggtgctcacc
     6421 tacctggagg cgaccggcgg ggcatcctcg cgctgaccga cgtcgggcgg tcgtgggtgt
     6481 gcggagagcc gcatcgcatc gggcggtcaa cctcctttgg caggaccgcc gtaccagtca
     6541 cgatggtgca caggaggtga ccgtggccga tgtccgcgtg ctcgtggtcg atgaccagga
     6601 gccgtttcgc cgggccgcag ccggggtcat cgacgagatg gccggcttcg tcgtggtcgg
     6661 atcggtggag tcggctgagc gctcgatcac tgctgtcgcg gagctgcacc ccgacctcgt
     6721 gttgatggac gtcaacctgc cggcgatgaa cggcatcgag gcagctcgcc gcctcacctc
     6781 ggccaacgcg ggcctcgtcg tgctgttgct ttcgacgtac gacgaggac
//
LOCUS       DATBFZ010000060         3459 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_39816, whole genome shotgun sequence.
ACCESSION   DATBFZ010000060 DATBFZ010000000
VERSION     DATBFZ010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 3459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3459
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39816"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1243
                     /locus_tag="VH419_01835"
     CDS             <1..1243
                     /locus_tag="VH419_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020386998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HEY7042387.1"
                     /translation="LDRAVVNSIATSMSTVLDDWDATNQKQSGRCWLFAGLNLLRAGA
                     AKSMDLKDFEFSQNYLLYWDKLEKANFWLEAIIETADRDVDDRTVAHLLANAAEDGGQ
                     WNMFVALVRKHGLVPKAAMPETHSSSKTHAMNRSISQLLRRSARDLRQQKANGATSEQ
                     LREAKLATLEQMHRVLSIHLGTPPTAVHWQWNDKDKNFHRDDTMTPQEFAEKYAELPI
                     DEYVCIVHDPRESSEVGRTYTVEYLGNIVGAPPVVYLNVDMSVIRELTMAAVSAGEPV
                     WFGCDTGKMSNLELGYWDEALYDYEGVYSFENGELSLDKADRLLHHDTMMTHAMLFTG
                     VDVVAGADGADAPRRWRVENSWGGDKADKGFWTMNDSWFGEHVFEVAVRRSALSAELQ
                     AALDQEPVILPAWDPMGALAR"
     gene            complement(1295..2008)
                     /locus_tag="VH419_01840"
     CDS             complement(1295..2008)
                     /locus_tag="VH419_01840"
                     /inference="COORDINATES: protein motif:HMM:NF014037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mut7-C RNAse domain-containing protein"
                     /protein_id="HEY7042388.1"
                     /translation="MADAIVAIQVAAPLRFFVPARRRAPRVEVAYDATSTIGHLVSSL
                     GVPLPEVGRLAVGGAQVSPAYRPQPGDLVVVAPVRRPQPLPGGRARFLLDVHLGSLAR
                     RMRLLGIDTRYERDIDDDELARLAPEDDRVLLTQDRGLLHRTAIRERAAYVRGSGADE
                     QLVDVLDRFALATAPFTRCLACNGLLAAASKQDVLPVLEPGTARTYDDFVRCQACGHV
                     YWRGAHARQLDALVAAART"
     gene            complement(2015..3130)
                     /locus_tag="VH419_01845"
     CDS             complement(2015..3130)
                     /locus_tag="VH419_01845"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HEY7042389.1"
                     /translation="MASPSRTVRVSRNPLARVMRRGGRTARRAIAETETTWLVVLISM
                     SLLFMVAGQVFDMWWPLTAYVIPVVLAMNVLSLARMVVLDIVVGVCLTISIAEFERGL
                     TRPAWAGIGVIVTVMVIVLWFAVDRQTLGVASTRGESMLIDLRDRLASQSQLPALPRA
                     WHAEAVMRSAGGASFAGDFIVAAKSITGDLLEVAVVDVSGKGVGAGSRALLLSGAFGG
                     LLGSLPEEEFLPAANEYLMRQEWSEGFATAVHMSINLKDGTFRVRTAGHPPALQFQAG
                     SGRWVVHWTEGPVLGLIHHADYTTHRGQMMPGDALLLYTDGLVETPKRDISYGIDKLL
                     GEAERLVQQGFVHGASRLVASVESESDDRALFLLHRR"
BASE COUNT          628 a         1195 c         1137 g          499 t
ORIGIN      
        1 cctcgaccgt gcggtggtga actccatcgc cacctcgatg tcgacggtgc tcgacgactg
       61 ggacgcgacc aaccagaagc agagcggacg ctgctggttg ttcgccgggc tcaacctgct
      121 gcgggccggc gccgccaaga gcatggacct gaaggacttc gagttctcgc agaactacct
      181 gctctactgg gacaagctcg agaaggccaa cttctggctg gaggcgatca tcgagaccgc
      241 tgaccgagac gtggacgacc gcactgtcgc gcacctgctc gccaacgccg ccgaggacgg
      301 cggccagtgg aacatgttcg tggccctggt ccgcaagcac ggcttggtgc cgaaggccgc
      361 gatgccggag acgcattcct cgtcgaagac gcacgcgatg aaccgctcga tcagccaact
      421 gctgcgacgc agtgcacgtg acctccgcca gcagaaggcc aacggcgcca ccagcgagca
      481 gcttcgcgag gccaagctcg ccacgctcga gcagatgcac cgcgtgctgt ccatccacct
      541 gggcacaccg ccgacggccg tgcactggca gtggaacgac aaggacaaga acttccaccg
      601 cgacgacacg atgacgccgc aggagttcgc cgagaagtac gccgagctcc cgatcgacga
      661 gtacgtctgc atcgtgcacg acccacggga gtccagcgag gtcgggcgca cctacaccgt
      721 cgagtacctc ggcaacatcg tcggcgctcc cccggtcgtc tacctcaacg tcgacatgtc
      781 cgtgatccgc gagctcacga tggcggcggt cagcgcaggg gagccggtct ggttcggctg
      841 cgacaccggc aagatgtcga acctcgagct cggctactgg gacgaggcgc tctacgacta
      901 cgaaggcgtg tactccttcg agaacggcga gctgtcactc gacaaggctg accggttgct
      961 ccaccacgac acgatgatga cccacgcgat gctcttcacc ggtgtggacg tcgtggccgg
     1021 agctgacgga gccgatgcgc cgcggcgctg gcgggtcgag aacagctggg gtggcgacaa
     1081 ggccgacaag ggcttctgga cgatgaacga cagctggttc ggcgagcacg tgttcgaggt
     1141 cgccgtacgc cgttcggcgc tgtctgccga gctgcaggcc gcactcgacc aagagcccgt
     1201 catcctcccc gcttgggacc cgatgggtgc tctcgcccgc tgatcgcccc gttgatcgct
     1261 gtcggcgatc gaacttgtcg agatccctgc cgcactaggt tcgcgccgcc gccaccagcg
     1321 cgtcgagctg tcgggcatgg gcgccccgcc agtagacgtg cccgcatgcc tgacatcgga
     1381 cgaagtcgtc atacgtgcgc gcggttccgg gttcgagcac cggcagcacg tcctgcttcg
     1441 aggccgccgc cagcagtccg ttgcaggcca ggcaccgagt gaacggcgcg gtcgcgagcg
     1501 cgaagcggtc gagcacgtcg accagctgct cgtcggcgcc tgatccgcgg acgtacgccg
     1561 cgcgctcgcg gatcgcggtc cgatggagca gccctcgatc ctgagtcagc aacacccggt
     1621 cgtcctccgg agcgagccga gccagctcgt cgtcgtcgat gtcgcgctcg tagcgggtgt
     1681 cgatcccgag cagccgcatc cgcctcgcca gcgaaccgag atgcacgtcg agcaggaacc
     1741 gggcgcgccc accgggcaag ggctgtggtc ggcgtaccgg agccaccacg acgaggtcgc
     1801 ccggctgagg acggtacgcc ggcgagacct gtgcaccacc gaccgccaag cggcccacct
     1861 caggcagcgg aacgcccaac gaggagacca ggtgaccgat cgtcgaggtc gcgtcgtagg
     1921 cgacctcgac gcgcggcgcc cgtcgtctgg ccggcacgaa gaagcgcagt ggcgcagcca
     1981 cctggatcgc gacgatcgcg tcggccatcc gtcctcagcg tcggtgcagc aggaacagcg
     2041 cccggtcgtc actctcggac tcgacgctcg cgaccaagcg gctggcgccg tgaacgaagc
     2101 cctgttgaac cagccgctcc gcctcgccga gcagcttgtc gatgccgtag ctgatgtccc
     2161 gcttcggggt ctcgaccagc ccgtcggtgt acagcagaag agcgtcgccg ggcatcatct
     2221 gcccgcggtg cgtcgtgtag tccgcgtggt ggatgagacc gaggacggga ccctcggtcc
     2281 agtgcacaac ccaacgccct gagccggcct ggaactgcag cgccggcgga tgaccggcag
     2341 tccgcactcg gaacgtgccg tccttcaggt tgatcgacat gtgtacggcg gtggcgaagc
     2401 cctcgctcca ctcctgcctc atcaggtact cgttggctgc cgggaggaac tcttcctcgg
     2461 ggagtgatcc cagcaacccg ccgaacgcgc cggacaagag gagcgcccgg gacccggctc
     2521 cgacaccctt cccggacacg tcgacgacgg cgacctcgag caggtcgccg gtgatggact
     2581 tggccgcgac gatgaagtcc cccgcgaacg acgcgccccc cgctgaccgc atcacggcct
     2641 cggcgtgcca ggcacggggc aacgcgggga gctggctctg gctggccagt cggtcgcgaa
     2701 ggtcgatcag catcgactca ccccgcgtcg aggccactcc cagtgtctgt cggtcgacgg
     2761 cgaaccagag cacgatcacc atcacggtca cgatcacgcc gatcccggcc caggcgggtc
     2821 gagtcaatcc gcgctcgaac tcggcgatgg agatcgtcag acacaccccc acgacgatgt
     2881 cgaggacgac catccgtgcg agtgacaaga cgttcatggc cagcacgaca gggatgacgt
     2941 aggccgtcaa gggccaccac atgtcgaaga cctgccccgc gaccatgaac agcagagaca
     3001 tgctgatcag cacgaccagc caggtggtct cggtctcggc gatcgcgcgc cgcgcagtcc
     3061 tgccaccacg ccgcatcacc cgcgcgagcg ggttgcggga cacccgaacg gtgcgcgagg
     3121 ggctggccac ggctgagagc gtagccaagt acgccgaccc cggctggaca ataggtccac
     3181 cgttggcgga ccgtgatgaa cgcctcacgt cgcgggcgca acgaaccagc tccacatggt
     3241 gaccggtcga tgtcggctgt tgtccatcga tgccgaacag gtcacgtttt gcccgcgtcg
     3301 acggaccacc caccgaggac taccgtcgcg acggatcgtc gccgcggccg agcggagaca
     3361 tcgagggagg gagtgatcga tgctcgcgat ctacgtcgat ttcgagtacg acgacgacgc
     3421 ccgggagctc ttgtccaagg tggcggccga gtccgtccc
//
LOCUS       DATBFZ010000061         2838 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_5927550, whole genome shotgun sequence.
ACCESSION   DATBFZ010000061 DATBFZ010000000
VERSION     DATBFZ010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2838
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5927550"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..504)
                     /locus_tag="VH419_01850"
     CDS             complement(<1..504)
                     /locus_tag="VH419_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEY7042390.1"
                     /translation="MKVLDGRTTSDLVKLLIFVVVTMLATSVLVMTIGNISFAPKQDY
                     KAEFVDATGVVKGDDVRVAGVKVGTVQDVQVQDRTRALVTFSVEDSASISKATHAAIR
                     YRNLVGQRYISLSQEIGDSAALHPGDTIPVSQTTPALDLTVLFNGFKPLFQALSPSDI
                     NKLSYEIV"
     gene            complement(501..1796)
                     /locus_tag="VH419_01855"
     CDS             complement(501..1796)
                     /locus_tag="VH419_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HEY7042391.1"
                     /translation="MARTSFATVALPKVMGIVFLCLVLAGVYLTYAIFSKKFTDYDTV
                     TLQSSTIGLQLPMRADVKVRGVIVGEVLDFKPTSDGADITLGIYPSKIDTIPADVTGS
                     IVPKTLFGEKYVSLVVPDKPEQGHLEAGQTIAKTEVSTEVEQVLSDLYPLLRTVQPAE
                     INQTLNALATALEGRGDELGENLETLDGYLKRINPQIPELVEDLRLTSEVSDNYADVM
                     PQIAQIIDDTVLTTGTLEDREQKLHALFQDVSSFSDTASDFLDENGDNLIRLSQVGPE
                     QLKVFARYAPEFSCLSKGLVNAGKREASAFRGFKLHIVLETLPKQPRAYGPEDQPRFG
                     DDRGPNCLHLPDPKWSQSNPVRHQPDFNDGVDEPTGKGTSRVVPNRSFQTATPGTSQE
                     SDLLRSLLGPALGVSADDVPDLGVLLVGPMARGAEVSLR"
     gene            complement(1802..2629)
                     /locus_tag="VH419_01860"
     CDS             complement(1802..2629)
                     /locus_tag="VH419_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY7042392.1"
                     /translation="MASIRAVYDRPARFLDNLGSELLFYVTALAWTPRTIRRYKKEVL
                     RILAEVTLGSGALAVIGGTVGVITAMCFFTGTEVGLQGYAALNQIGTAAFSGFVSAYF
                     NTREIAPLVAGIALAATVGCGFTAQLGAMRISEEVDALEVMAVPSLPFLVTTRIIGGL
                     IAIIPLYVVGLLASYFATRLTVTTFFGQSTGTYDHYFNQFLPPGDVLWSFGKVLVFAV
                     TVILIHCYHGYNATGGPAGVGVAVGRAVRTSIVALNVIDLLLSMAIWGTTTTVRLAG"
     gene            complement(2635..>2838)
                     /locus_tag="VH419_01865"
     CDS             complement(2635..>2838)
                     /locus_tag="VH419_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003951411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY7042393.1"
                     /translation="WIGMIKALVFGMLAAIVAAYKGMSAGGGPKGVGDAVNESVVVTF
                     MLLFLVNFVMSAIYMQLVPPKTG"
BASE COUNT          466 a          893 c         1020 g          459 t
ORIGIN      
        1 gacgatctcg taggacagct tgttgatgtc cgacggcgac agcgcctgga acaacggctt
       61 gaacccgttg aacagcacgg tcaggtcgag cgcgggcgtg gtctggctga ccgggatcgt
      121 gtcgccgggg tgcagcgcgg cggagtcgcc gatctcctgg ctgagcgaga tgtagcgctg
      181 accgacgagg ttccggtacc ggatcgcggc gtgcgtcgcc ttgctgatcg aggcggagtc
      241 ctcgacgctg aacgtcacca gcgcccgggt ccggtcctgg acctggacgt cctggaccgt
      301 gccgaccttc acgccggcga ccctgacgtc atcgcccttc acgacgccgg ttgcgtcgac
      361 gaactccgcc ttgtagtcct gcttgggcgc gaacgagatg ttgccgatcg tcatcaccag
      421 gaccgacgtg gcgagcatcg tgaccacgac gaagatgagg agcttgacca ggtctgaggt
      481 ggtgcggccg tcgaggacct tcatcgcagc gacacctccg ccccgcgtgc catcgggccg
      541 accagaagga caccgaggtc cggtacgtcg tcggcgctca cgccgagcgc cggaccgagc
      601 agcgagcgca gcaggtcgga ctcctgcgag gtgcccggcg tggcggtctg gaaggaccgg
      661 ttcggcacga cccggctggt gcccttgccg gtcggctcgt cgacgccgtc gttgaagtcg
      721 ggctggtgcc gcaccgggtt ggactggctc cacttcgggt cgggcaggtg caggcagttg
      781 ggaccgcggt cgtcgccgaa ccggggctgg tcctccggac cgtacgcgcg cggctgcttc
      841 ggcagcgtct ccagcacgat gtgcagcttg aagccgcgga acgccgacgc ctcccgcttg
      901 ccggcgttga cgaggccctt ggacaggcag ctgaactccg gcgcgtagcg ggcgaagacc
      961 ttcagctgct cggggccgac ctggctgagc cggatcaggt tgtcgccgtt ctcgtcgagg
     1021 aagtcgctgg cggtgtcgga gaaggacgag acgtcctgga agagtgcgtg cagcttctgc
     1081 tcccggtcct ccagcgtgcc ggtcgtcagc acggtgtcgt cgatgatctg ggcgatctgc
     1141 ggcatcacgt cggcgtagtt gtcggagacc tccgaggtca gacgcaggtc ctcgaccagc
     1201 tcggggatct gcgggttgat gcgcttgagg tagccgtcga gcgtctcgag gttctccccc
     1261 agctcgtcac cgcgcccctc gagggcggtc gcgagcgcgt tcagggtctg gttgatctcg
     1321 gcgggctgca cggtgcggag cagcgggtag aggtcggaga gcacctgctc gacctcggtc
     1381 gagacctcgg tcttcgcgat cgtctggccg gcctcgaggt gcccctgctc gggcttgtcg
     1441 gggacgacga gcgagacgta cttctcgccg aacagcgtct tcggcacgat cgacccggtg
     1501 acgtccgccg ggatggtgtc gatcttgctg gggtagatgc cgagcgtgat gtcggcgccg
     1561 tcgctcgtcg gcttgaagtc gagcacctcg ccgacgatca cgccacgcac cttcacgtcg
     1621 gcacgcatcg gcagctgcag cccgatcgtc gacgactgca gggtcaccgt gtcgtagtcg
     1681 gtgaacttct tggagaagat cgcgtaggtg aggtagaccc cggccagcac caggcagagg
     1741 aacacgatgc ccatcacctt gggcaacgcg acggtggcga acgacgtacg cgccatcggc
     1801 gtcaccccgc cagccgcacg gtcgtggtgg tcccccagat ggccatggac agcaggaggt
     1861 cgatgacgtt gagcgcgacg atgctggtgc ggaccgcacg gcccacggcg acgccgacgc
     1921 cggcggggcc gccggtcgcg ttgtagccgt ggtagcagtg gatcaggatc acggtgacgg
     1981 cgaagacgag caccttgccg aaggaccaga gcacgtcacc gggtggcagg aactggttga
     2041 agtagtggtc gtaggtgccg gtgctctgcc cgaagaaggt cgtgacggtc agccgggtcg
     2101 cgaagtagga cgccagcagg ccgacgacgt agagcgggat gatcgcgatc aggccgccga
     2161 tgatgcgggt ggtgaccagg aacggcagcg acgggaccgc catcacctcg agcgcgtcga
     2221 cctcctcgga gatccgcatc gcgccgagct gggcggtgaa gccgcagccg acggtggcgg
     2281 cgagcgcgat gccggcgacg agcggtgcga tctcgcgggt gttgaagtac gccgagacga
     2341 agcccgagaa cgccgcagtg ccgatctggt tgagcgcggc gtacccctgc aggccgacct
     2401 cggtgccggt gaagaagcac atcgcggtga tgactcccac ggtgccgccg atgaccgcga
     2461 gcgcgccgga gcccagggtg acctccgcca ggatgcggag gacctccttc ttgtagcgcc
     2521 ggatcgtgcg cggcgtccac gcgagcgcgg tcacgtagaa gagaagctct gagccgaggt
     2581 tgtcgaggaa cctggctggc cggtcgtaga cggccctgat gctcgccatc acgctcaccc
     2641 tgtctttgga gggacgagct gcatgtagat cgcgctcatc acgaagttga cgaggaacag
     2701 aagcatgaag gtgacgacga cggactcgtt gaccgcgtca ccgacgccct tgggcccgcc
     2761 gccggccgac atgcccttgt aggcggcgac gatggcagcc agcatcccga acaccagcgc
     2821 cttgatcatg ccgatcca
//
LOCUS       DATBFZ010000062         2070 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_1025438, whole genome shotgun sequence.
ACCESSION   DATBFZ010000062 DATBFZ010000000
VERSION     DATBFZ010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1025438"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..933)
                     /locus_tag="VH419_01870"
     CDS             complement(<1..933)
                     /locus_tag="VH419_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042394.1"
                     /translation="MLVMDSRMQTMDGVTLFADHADRSQFWYLPFPVRLAERDGEPQF
                     TLIRYRPAVADSGVKGGGFLMMEVELRLDPEVEQRLRSAASRLSDGVPRLSVVPFDEG
                     TVQVAVLNLQGGGGTTAPTPPPGALLAVTSILGANTPSLAGNNSAVFSLTLSQEGSVI
                     LDQAFRQGAMPVGVLYNLKYTVLQPALDVEITANFKRIYDHLSFGVDLSASAVIYGVP
                     VYLEAGIDMAFEKLKQDGVIIIKVINFSDAADEADKEKWALDFFKSMLLEQWFKPTLA
                     PVTFDQKPKTGPATPGGASGAGASGSGASGSGASG"
     gene            complement(972..>2070)
                     /locus_tag="VH419_01875"
     CDS             complement(972..>2070)
                     /locus_tag="VH419_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042395.1"
                     /translation="LTYDGQPRTIRLTEEAPDGDSDWNSLVTNGRMVRDVAYEYRVSF
                     DGVDSAQRPAVLLSGTRTTIGDQFEVSPRAEELYFLDDIQIGAALLPWDRYPQVAVEV
                     RYADPPNRIRLAETFLLSKERPEATWTRFRLNPALTGYDVRVTFLSPAGRDVLQDWTT
                     TDQERLVIRDPHPLRRTVTVAPAVDWRLVAMVFVELRYVDLPNSVDEEATLSFFDTPE
                     DRAPKTFAVNLVDGEQRLVRWSATFVLKDNRTIAVPPSMTAGSTVVLRTDMAGHRVVT
                     VLPPEVDFTARGVARVEARLAYDDAQSALHFEDEYTFAGPGEARFFEYDYVADDRSGY
                     SCQLRTVLANGLVQERDLGRLDVDRLVLPVA"
BASE COUNT          313 a          714 c          720 g          323 t
ORIGIN      
        1 ccccgacgcc ccactccccg acgccccact ccccgacgcc ccagcccccg acgcccctcc
       61 gggtgtggcc gggccggtct tcggcttctg gtcgaaggtg acgggcgcga gcgtcggctt
      121 gaaccactgc tcgaggagca tcgacttgaa gaagtcgagc gcccacttct ccttgtcggc
      181 ctcgtcggcg gcgtccgaga agttgatgac cttgatgatg atgacgccat cctgcttgag
      241 cttctcgaag gccatgtcga tccccgcttc caggtagacc ggcacgccgt agatcaccgc
      301 gctggccgac aggtcgacgc cgaagctcag gtggtcgtag atgcgcttga agttcgcggt
      361 gatctccacg tcgagcgctg gctgcagcac ggtgtacttc aggttgtaga ggacaccgac
      421 cggcatcgcg ccctggcgga aggcctggtc gaggatgacc gacccctcct ggctcagcgt
      481 gaggctgaac accgccgagt tgttcccggc gagtgacggg gtgttggcgc cgaggatgga
      541 ggtcaccgcg agcagggccc ccgggggcgg ggtgggtgcc gtggtgccgc cgcctccctg
      601 caggttcagc accgcaacct gaactgtgcc ctcgtcgaag ggcaccacgc tcaaccgcgg
      661 cacgccgtca ctgagcctcg aagcggcgct gcggagccgc tgctcgacct ccgggtccag
      721 ccgcagctcg acctccatca tcaggaagcc gcctcccttg acgccgctgt cggccacggc
      781 cgggcggtag cggatcagcg tgaactgtgg ctcgccgtcg cgctcggcca gccggaccgg
      841 gaaggggagg taccagaact ggctccggtc cgcgtggtcg gcgaagagcg tgacaccgtc
      901 catggtctgc atccggctgt ccatcacgag catcggacgt acccccttct ttcgcgagct
      961 tgcgccgctc gctaggccac cggcagcacg agccggtcca cgtccaggcg ccccaggtca
     1021 cgttcctgca cgagcccgtt ggcgaggacc gtgcgcagct ggcaggagta gcccgagcgg
     1081 tcgtcggcca cgtagtcgta ctcgaagaac cgcgcctccc ccgggccggc gaacgtgtac
     1141 tcgtcctcga agtggagcgc cgactgcgcg tcgtcatagg caaggcgagc ctcgaccctc
     1201 gcgacccccc gggcggtgaa gtccacctct ggcggcagca ccgtgacgac ccggtgcccc
     1261 gccatgtcgg ttcgcagcac aaccgtggac ccagcggtca tcgacggtgg cacggcgatc
     1321 gtgcggttgt ccttgaggac gaaggtggcg ctccagcgga ccagccgctg ctcgccgtcc
     1381 accaggttga cggcgaaggt cttgggcgcc cggtcctcgg gggtgtcgaa gaacgacagc
     1441 gtcgcctcct cgtccaccga gttgggcagg tcgacgtagc gcaactcgac gaacaccatg
     1501 gcgaccagtc gccagtcgac cgctggcgcc acggtcacgg tgcgccgcag cgggtgcggg
     1561 tcccggatga cgagccgttc ctggtcggtg gtcgtccagt cctgcaggac gtcccggccg
     1621 gcgggcgaca ggaacgtcac ccggacgtcg tagccggtga gcgccgggtt cagccggaag
     1681 cgggtccagg tggcctcggg gcgctccttg ctgagaagga acgtctcggc cagccggatc
     1741 cggttgggcg ggtcggcgta ccgcacctcg accgcaacct gcggataccg gtcccacggg
     1801 agcagggcgg cgccgatctg gatgtcgtcg aggaagtaca gctcctcggc gcggggcgag
     1861 acctcgaact ggtcgccgat cgtggtgcgg gtgccggaca gcaggacggc gggccgctgc
     1921 gcgctgtcga cgccgtcgaa gctcacgcga tactcgtagg cgacgtcacg gaccatccgg
     1981 ccgttcgtga ccaagctgtt ccagtcggag tccccgtccg gggcctcctc ggtcagccgg
     2041 atggtgcgcg gctggccatc gtaggtgagg
//
LOCUS       DATBFZ010000063         1669 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_1026324, whole genome shotgun sequence.
ACCESSION   DATBFZ010000063 DATBFZ010000000
VERSION     DATBFZ010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 1669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1669
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1026324"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1619)
                     /locus_tag="VH419_01880"
     CDS             complement(<1..1619)
                     /locus_tag="VH419_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042396.1"
                     /translation="MKAIYDLSATPSFLSGSGHREGTLFPWVVSDFGLIEAIESGIVK
                     IPRVPVDDNAVSVDVKYLNLWPNVGDQLPKRGRKDVEVPQNLPGLLEGALESLYDSYD
                     KAFRQWEGSQIARDGEPPPVFIVVCNNTTVSKMVYDYIAGWERRAAGGDGPSVLVPGR
                     LALFSNVRNGEWRHRPRTILVDSQQLESGEALKPEFKQVAAAEIEEFTADYLRRFPGR
                     SADDVGDAEILREVMNTVGKKDKLGEPVRCVVSVSMLTEGWDANTVTHILGVRAFGTQ
                     LLCEQVVGRGLRRRSYAADENGFFTPEYADVYGVPFQFIPTVAKTRDITMKPNRRVHA
                     APDRAAAQITFPRLVGYRIELPDEELWAEFTERSRLVLSTSDMPTETVVSGLIGSAGT
                     HTLDELRAKRDQEIAFHLTRRIMERFLAGQDPKPWYFPQLLRITKRWLAECLTCDSQT
                     FPGLLLIGENADRAASLILHEVVSSQPGNRRPMVRPMLAPDPAGSTATVDFYTTREVY
                     PTSEQRCHVNFAVLDGPRGNTWEEMVAQLLEA"
BASE COUNT          260 a          555 c          550 g          304 t
ORIGIN      
        1 gcctccagaa gctgggcgac catctcctcc caggtgttgc cgcgcgggcc gtcaagcacc
       61 gcgaagttca cgtggcagcg ctgttccgag gtcgggtaga cctcccgtgt ggtgtagaag
      121 tccacggtcg cggtcgagcc agccggatca ggggccagca tcggccggac catcggccgc
      181 cggttgcccg gctgggagct gacgacctca tgcaggatca ggctggctgc ccggtcggcg
      241 ttctcgccga tcagcagcag gccggggaat gtctggctgt cgcaggtcag gcattcggcc
      301 agccaccgct tggtgatccg aagcagttgc gggaaatacc acggcttggg gtcctgtccg
      361 gccaggaacc gttccatgat ccgccgcgtc agatggaacg cgatttcctg gtcccttttg
      421 gcccgcagct cgtctagcgt gtgcgttcct gccgagccga tcaggccgct gactaccgtc
      481 tcggtcggca tgtcggaagt gctcagtaca agccgactgc gttcggtgaa ctcggcccag
      541 agttcctcgt cgggcagctc gatccggtag ccgaccagcc gcgggaaagt tatctgtgcc
      601 gctgcccggt ctggcgcagc gtggacccgc cggttcggct tcatcgttat gtcacgggtc
      661 ttggcgaccg tggggatgaa ctggaacggc accccgtaca cgtcggcgta ctccggggtg
      721 aagaacccgt tttcatccgc cgcgtaggag cggcgccgca gtccacggcc gactacctgc
      781 tcgcacagta gctgggtgcc gaacgcccgc acaccaagga tgtgggtgac cgtgttggcg
      841 tcccagccct cggtgagcat ggatacgctg accacgcagc gcaccggctc gccgagcttg
      901 tctttcttgc cgaccgtgtt catgacctcg cgcaagatct cggcgtcgcc gacgtcgtcg
      961 gcagagcggc ccgggaagcg gcgcaggtag tcggcggtga actcctcgat ctcggcggcg
     1021 gcgacctgct tgaactcggg cttgagcgct tcgccggatt ccagttgctg ggagtcgacc
     1081 aggatggtgc gtgggcggtg ccgccattcc ccgttccgca cgttggagaa cagcgcgagc
     1141 ctgccgggca ccaacacgga cggaccatcg ccgcccgccg cccgccgttc ccagccggcg
     1201 atgtagtcgt agaccatctt cgacacggtg gtgttgttgc atacgacgat gaacactggc
     1261 ggtggctcgc cgtccctggc tatctgcgag ccctcccact gccggaacgc cttgtcgtag
     1321 gagtcgtata ggctttccag cgccccctct agcaggccgg ggaggttctg cggtacctcg
     1381 acatccttgc ggccccgctt cggcagctgg tccccgacgt tcggccacag gttcaggtac
     1441 ttcacgtcga ccgagaccgc gttgtcatct accggcaccc gggggatctt cacgatcccg
     1501 gactcgatcg cctcgatgag accgaagtcg ctgaccaccc atgggaagag cgtgccttcc
     1561 cggtggccgg acccggacag gaacgacggg gtggcggaca ggtcatagat cgccttcacg
     1621 ccgagcttct cggcgatggc ccgcagcccg gtaaaccaca cccgggcgg
//
LOCUS       DATBFZ010000064         1500 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_2011007, whole genome shotgun sequence.
ACCESSION   DATBFZ010000064 DATBFZ010000000
VERSION     DATBFZ010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2011007"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="VH419_01885"
     CDS             <1..286
                     /locus_tag="VH419_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007076579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042397.1"
                     /translation="VGGLPVAVADGVSGALVEGHDVDDWAAAIDDVLQRDPGPLSVAA
                     AAHAAQFSWGHTVDALLNSYAHAMSDYRSRHRRPGRRPGRRFALRRGVRA"
     gene            289..789
                     /locus_tag="VH419_01890"
     CDS             289..789
                     /locus_tag="VH419_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020578987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HEY7042398.1"
                     /translation="MGTSVEQIIITTLDEHELVYQRHEGAHGGLPGIVVELPGERKLK
                     TNTILSIGEHSVRVEAFVCRKPDENFEGVYRFLLKRNRRLYGVAYTLDNVGDIYLVGR
                     MALEAVSADEVDRVLGQVLEAVDSDFNTLLELGFRSSIQKEWEWRVSRGESLKNLEAF
                     EHLIED"
     gene            813..>1500
                     /locus_tag="VH419_01895"
     CDS             813..>1500
                     /locus_tag="VH419_01895"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007030857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglyceromutase"
                     /protein_id="HEY7042399.1"
                     /translation="MPTLILLRHGESDWNQKNLFTGWVDVDLTDKGRTEAVRGGKLLV
                     ENDVLPDVVYTSLLRRAITTANLALDAADRHWIPVHRDWRLNERHYGALQGLDKAATK
                     EKYGEEQFMAWRRSYDTPPPAIEKGSEFSQDSDPRYADIGGGPLTECLKDVVTRFVPY
                     YEDTIVPDLKAGKTVLIAAHGNSLRALVKYLDGMSDEEVVGLNIPTGIPLRYELDENL
                     KPLVAGGEYLD"
BASE COUNT          273 a          505 c          504 g          218 t
ORIGIN      
        1 ggtcggcggt cttccggtcg cggtggcaga cggagtcagc ggggccctgg tcgaaggtca
       61 cgatgtcgac gactgggcgg ccgcgatcga cgacgtgctc cagcgcgatc cggggccgct
      121 gagcgtggcc gccgcggcgc acgccgcgca gttctcctgg ggacacaccg tcgatgccct
      181 gctgaacagc tatgcccatg cgatgagcga ctaccggtcc cggcaccgca ggcccggccg
      241 ccgtcccggg cggcgattcg ccctgcgtcg gggggtacgg gcgtgaccat gggcaccagc
      301 gtcgaacaga tcatcatcac caccctcgac gagcacgaac tcgtctatca gcggcacgag
      361 ggcgcccatg gtggcctgcc gggcatcgtc gtcgagctgc cgggggaacg gaagctcaag
      421 accaacacca tcctgagcat cggcgagcat tcggttcgcg tcgaggcgtt cgtctgccgc
      481 aagcccgacg agaatttcga gggcgtctac cggttcctgc tcaagcgcaa ccgcaggctc
      541 tacggcgtcg cctacaccct cgacaacgtg ggcgacatct acctcgtcgg ccggatggca
      601 ctggaagcgg tcagcgccga cgaggtcgac cgcgtgctcg gccaggtgct cgaggccgtc
      661 gactccgact tcaacacctt gctggaactg ggcttccgct cctccatcca gaaagagtgg
      721 gaatggcggg tgtcgcgcgg tgagtcgctg aagaacttgg aggcgttcga gcacctgatc
      781 gaggactagg tgctcgtgag aagatcacgg gcatgccgac gctgatcctg ctccgccacg
      841 gcgagagcga ctggaaccag aagaacctgt tcacgggatg ggtcgacgtc gacctcaccg
      901 acaagggccg caccgaggcc gtccgcggcg gcaagctgct ggtcgagaac gacgtgctgc
      961 ccgacgtggt ctacacctcg ctgctgcgcc gcgcgatcac caccgcgaac ctcgccctgg
     1021 atgccgccga ccggcactgg atccccgtgc accgcgactg gcggctcaac gagcggcact
     1081 acggcgcact gcagggcctc gacaaggccg cgaccaagga aaagtacggc gaagagcagt
     1141 tcatggcatg gcggcgcagc tatgacaccc cgccgccggc catcgagaag ggcagcgagt
     1201 tcagccagga ctccgacccc cgctacgccg acatcggtgg cggaccgctg accgaatgcc
     1261 tcaaggacgt cgtgacccgg ttcgtgccgt actacgagga cacgatcgtg cccgacctga
     1321 aggccggcaa gaccgtgctg atcgcggccc acgggaactc gctgcgtgcg ctggtcaagt
     1381 acctcgacgg gatgtccgac gaagaggtcg tcggcctgaa catcccgacc ggcatcccgc
     1441 tgcgctacga gctggacgag aacctgaagc cgctggtcgc gggcggtgag tacctggacc
//
LOCUS       DATBFZ010000065         4408 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_1681452, whole genome shotgun sequence.
ACCESSION   DATBFZ010000065 DATBFZ010000000
VERSION     DATBFZ010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 4408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4408
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1681452"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..4313)
                     /locus_tag="VH419_01900"
     CDS             complement(<1..4313)
                     /locus_tag="VH419_01900"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HEY7042400.1"
                     /translation="MQAHEPGDYLIGQRWAAPATGPQTLTVVAQRNLDWTTMTLLDFD
                     RDGRPDAMVNFAWSPGQQDVVWQLWRLTPAAMAPGIGTTGGLPCFTPTPDDATLVGAG
                     HIPEVRRTFTWEVTLTHDFGADLGSAYAGLVRYQPIITTIFTPSARALFSAYTVNDIL
                     PNSADPVQAEGDTAHQCSGKVNGTFSEGIQVAPARGTDRGQSTFLPARRQAMFTDATT
                     TDTGQPQLVESDIEQWNASQTTDDLTADVPNPTPQTYHPADLVLTMRADHPLDTVTYL
                     VPDGGKQPIGLVSTSGVANQPAGQATLHVQLASGLSSLSYPNSATGNGCYPRSASTII
                     NGHAWGPTPVSFDVPLTAVTGDNGQSEYRAEISLDDVLGIGDGGTGDRPAFRWATTSA
                     PGLHFPPQYTPDVAGLDDIDQPFGVCTVPTTGDPDGTQLWPSSMVRMDTTLPSASLAS
                     SPSNPHAGDTVTLTATTDATSPSYRFDPESTGSWGSFGASASTTHAYGSTVGGQPFVA
                     HVYLVNGAGTGAMASTTVATDDPPPTASFSESSGSPAPPFVIGTNGPTISWTDQSTDT
                     LGGVIKAWHWDLHKTTWNGTGGTSCDATATDSSDPSCDAAGFSHTFVTGDQGDWVLTE
                     TVTDDGGAKSSMFKTFHVVQPPIALLDSVTPQPIVINLNTFPVEGDFTIHMKTADDGS
                     LGSEGPLTYFFAGASGTLATPPFGANDFFQGSDPFDGTFDGKEFRTGVFPVQAEVEDT
                     DGRTDYTPVFNVLVRGAGDSPPTAHLTTSPGTIRSGETDVTFDASASVLNNPDGSTGV
                     PDHFQFDFGDGTPPLDTTSAVVHHTYAGSGQYTASVTVIDDRILPATPSDPATANLTV
                     APGTTDTSAPVAKLALVHPDQPVFAKAPVELTAAASTVHAGDGPAHFAWDLDGDGTFE
                     TDTGTNATLNTTYPTAGVRHVKVRVTDARGRASVSNDLFVGVNPPNDQPPFVQVTGPD
                     TITLANGSGQATFDASNSVGRNLDGSLTFAWDLNGDGTYETFTGSVSHVTATFTSSGV
                     HTVRVRATDIYGNSDVASTTIFVRGAAEVAAGCQSKEQYRTLSYGKIQLAACWSQVDR
                     PGTAPLWVARNNVLVDGMLLNSPTAGSADQQTFSNCTGSCATTQSAFNLHHGSMIAFD
                     PTNGLLATNMPFAWRATNGVESFVLNQGSLDLMLPTSSDADGLILHPPSGGTFLTLSV
                     ASQAEVKFPDDGEATIALTMHMPPQLPGASGDLTLRSTTTQGLIVDHLAVDVTTGLLA
                     DKLKLSDLSLEYDRPTQLWSGSAELGIPGIKKKPDFGLKVGVSVQNGKFHSIYGEVNG
                     LEIDLGEGIFLQRIAAGVGVDPIDIQGGLGISAGPEILGTQILSADGDFRLTLPSAAA
                     PYTLFQIAGVTKLADLFDLTKGVARFTTDGFVEARGGLTRESFIGYFDASIGGWFTLH
                     D"
BASE COUNT          661 a         1415 c         1590 g          742 t
ORIGIN      
        1 aagtcgtgga gcgtgaacca tcctccgatc gacgcgtcga aatagccgat gaacgactcg
       61 cgggtgagcc cgccgcgggc ctcgacgaag ccgtcggtcg tgaagcgagc gacgcccttg
      121 gtgagatcga acaggtccgc gagcttcgtt acacccgcga tctggaacag cgtgtacggc
      181 gcggcggccg agggcagcgt gagccggaag tcgccgtcgg cggagaggat ctgggtgccg
      241 aggatctccg gcccggcgct gatgccaagt ccgccctgga tgtcgatcgg atcgaccccg
      301 acgccggccg cgatccgctg caggaagatg ccctcgccga ggtcgatctc gaggccgttg
      361 acctcgccgt agatcgagtg gaacttgccg ttctgcacgc tgacaccgac cttgaggccg
      421 aagtcgggct ttttcttgat ccccggaatc ccgagctcgg cggagcccga ccagagctga
      481 gtgggacggt cgtattcgag actcaggtca gacagcttga gcttgtcggc gagcagcccg
      541 gtggtgacgt cgaccgcgag gtggtcgacg atcagcccct gcgtcgtcgt cgaacgcagg
      601 gtcagatcac cactggcgcc gggaagctgc ggtggcatgt gcatcgtcag cgcgatcgtc
      661 gcctcgccgt cgtcggggaa cttcacctcg gcctggctcg cgacgctcag cgtcaggaag
      721 gtgccgcccg acggcgggtg caggatcagg ccatcggcgt cgctggacgt gggcagcatc
      781 aggtcgagcg acccctggtt gagaacgaag ctctcgacgc cgttcgttgc cctccacgcg
      841 aacggcatgt tcgtggcgag caggccgttg gtcggatcga acgcgatcat cgacccgtgg
      901 tggaggttga acgcagactg ggtcgtcgcg cagctccctg tgcagttcga gaacgtctgc
      961 tggtcggcgg aaccggcggt gggcgagttc agcagcatgc cgtcgaccaa cacgttgttg
     1021 cgcgcaaccc acaacggcgc cgtgcccggt cggtcgacct gcgaccagca ggcggcgagc
     1081 tggatctttc cgtaggacag cgtccggtac tgctctttgc tctgacaccc cgccgcaacc
     1141 tcggccgcgc cccgaacgaa gatcgtcgtc gaggcgacgt ccgagttgcc gtagatgtcg
     1201 gtcgcgcgga cccgtacggt gtggacgccc gaggaggtga acgtggcggt cacgtgggag
     1261 accgagccgg tgaacgtctc ataggtcccg tcgccgttga gatcccaggc gaacgtgagc
     1321 gagccgtcga ggttgcgccc gaccgagttc gacgcatcga acgtcgcctg gcccgacccg
     1381 tttgccagcg tgatcgtgtc ggggccggtg acctgaacga acggcggctg gtcgttgggc
     1441 ggattgaccc cgacgaacag atcgttggag accgaagcgc ggcctcgcgc gtccgtgacg
     1501 cggaccttga cgtgacgcac accggccgtc ggatacgttg tgttgagggt cgcgtttgtt
     1561 cccgtgtcgg tctcgaacgt gccgtcgccg tcgaggtccc acgcgaagtg cgcgggcccg
     1621 tcacccgcat ggacggtcga cgccgccgcg gtcagttcca ccggcgcctt cgcgaacacc
     1681 ggctggtccg gatggacgag cgcgagcttc gcgaccgggg cgctggtgtc ggtcgtgccc
     1741 ggcgcgaccg tcaggttggc ggtcgccgga tccgacggcg tcgcgggcag gatgcggtcg
     1801 tcgatgacgg tgactgacgc cgtgtactgc ccggagcccg cgtaggtgtg atggacgacc
     1861 gccgaggtgg tgtcgagcgg cggcgtgccg tcgccgaagt cgaactggaa gtgatcgggg
     1921 acgcccgtcg agccgtccgg gttgttcagc accgatgccg acgcgtcgaa ggtcacgtcg
     1981 gtctcgccgg atcggatcgt gcccggcgag gtcgtgaggt gggcggtcgg cggcgagtcg
     2041 ccggcgccgc ggacgagcac gttgaagacc ggcgtgtagt cggtccgtcc atcggtgtct
     2101 tcgacctcgg cctggaccgg gaagactccg gttctgaact ccttgccgtc gaacgtgccg
     2161 tcgaacggat cggacccctg gaagaagtca ttggccccga acggtggggt cgccaacgtt
     2221 ccggacgcgc ccgcgaagaa gtaggtcagc ggaccctcgc tgccgagcga cccatcgtcc
     2281 gccgtcttca tgtgaatcgt gaagtcgccc tccaccggga acgtgttcag gttgatcacg
     2341 atcggttgcg gcgtgacgct gtccagcagc gcgatcgggg gctggacgac gtggaacgtc
     2401 ttgaacatgc tcgacttcgc gccgccgtcg tcggtcacgg tctcagtcag cacccagtcg
     2461 ccctggtcgc cggtcacgaa cgtgtgcgag aagccggccg cgtcgcagct cgggtcgctg
     2521 gagtcggtgg cggtcgcgtc gcacgaggtc ccgccggtgc cgttccaggt cgtcttgtga
     2581 aggtcccagt gccacgcctt gatgacaccg ccaagcgtgt ctgtcgactg gtcggtccag
     2641 ctgatcgtcg gaccgttcgt accgatcacg aacggcggcg ccggactccc gctgctctcg
     2701 ctgaaggatg cggtcggcgg cgggtcgtca gtggcgaccg tcgtcgatgc catcgcgccg
     2761 gtgcccgcgc cgttgacgag gtacacgtgc gcaacaaacg gctgcccgcc gacggtcgac
     2821 ccatacgcgt gggtcgtcga ggcgctggcg ccgaagctcc cccaggatcc ggttgattcc
     2881 gggtcaaagc ggtaggacgg gctcgtcgca tcggttgtcg ccgtgagcgt gaccgtgtcg
     2941 ccggcgtgtg gattggacgg gctcgaggcg agcgaggcgc ttggcagcgt ggtgtccatg
     3001 cgcaccatcg aggacggcca cagctgcgtg ccatccgggt cgcccgttgt cgggacggtg
     3061 caaacgccaa agggctgatc gatgtcgtcg agcccggcca catccggcgt gtactgaggc
     3121 gggaagtgca gccccggggc cgaggtcgtg gcccaccgga acgccggccg atcgccggtg
     3181 ccgccgtcgc cgatgccgag cacgtcgtcg agcgagatct ccgctcggta ctcggattgc
     3241 ccgttgtcgc cggtgaccgc ggtcaacggc acgtcgaagc tgaccggtgt cggcccccaa
     3301 gcgtgaccgt tgatgatcgt gctcgcactg cgcggatagc agccgttgcc ggtggccgag
     3361 ttgggatacg agaggctcga caggcccgat gcgagctgga cgtgcagcgt tgcctgcccc
     3421 gccggctgat tggcaacgcc gctggtgcta acgaggccga tcggctgctt tccgccgtcc
     3481 ggcacgagat acgtcaccgt gtcgagcgga tggtcggcgc gcatcgtcag gacgaggtcc
     3541 gccgggtgat acgtctgagg agtcggattg ggcacatccg cggtcaggtc atcggtcgtc
     3601 tgcgacgcgt tccactgctc gatgtcggac tcgacgagct gcggctggcc ggtgtcggtt
     3661 gtcgtcgcgt cggtgaacat cgcctggcgg cgcgccggca ggaacgtcga ctgaccacgg
     3721 tcggtgcccc tggcgggcgc cacctggatg ccctcggaga acgtgccgtt gaccttcccg
     3781 gagcactggt gcgccgtgtc gccctctgcc tgcaccggat ccgccgagtt cggcaggatg
     3841 tcattgaccg tgtaggcgct gaacagcgcc cgtgcgctgg gggtgaaaat cgtcgtgatg
     3901 atcggctggt agcgcacaag gccggcgtac gcgctgccga ggtcggcgcc gaagtcgtgc
     3961 gtcagcgtca cctcccaggt gaacgtgcgt cgcacctcgg gtatgtggcc ggcgccgacg
     4021 agcgtggcgt cgtcgggcgt cggcgtgaag cacgggagcc cgccggtggt cccgatgccc
     4081 ggggccatcg ccgcgggcgt cagccgccac agttgccaga cgacgtcctg ctggccggga
     4141 ctccaggcga agttgaccat cgcgtccggc ctgccgtcgc ggtcgaagtc cagcagcgtc
     4201 atggtcgtcc agtcgaggtt gcgctgcgcg acgaccgtga gggtctgcgg accggtcgcc
     4261 ggggcggccc accgttggcc gatcaggtag tcgccgggct catgggcctg cacgacggtc
     4321 gccttggcgc tgcctgcgaa cacaagcgcg agcagcccgc aaatcactgc aaccagcaca
     4381 cccgcggctc ggctcctcac cgagcgac
//
LOCUS       DATBFZ010000066        16840 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_2668448, whole genome shotgun sequence.
ACCESSION   DATBFZ010000066 DATBFZ010000000
VERSION     DATBFZ010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 16840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16840
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2668448"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..981)
                     /locus_tag="VH419_01905"
     CDS             complement(<1..981)
                     /locus_tag="VH419_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016669877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HEY7042401.1"
                     /translation="MSNRVASLRAAVAERVVVADGAMGTMLQQQDLTLDDFEGLEGCN
                     EILNVSRPDVVRVIHDAYFAAGADCVGTNTFGANFANLSEYDIVPRIRELARTGAQIA
                     RDAADAAATPARPRWVLGSVGPGTKLPTLQHVTFATLRDAYQEQVRGLIEGGVDAVLV
                     ETAQDLLQAKAAVIGAKRAIAEGAYADRQDQPLLIVNVTVETTGTMLLGTEIGAALTA
                     LEPLDIDMMGLNCATGPAEMSEHLRYLSRQAAVPLACMPNAGLPQLTAEGASYPLTPV
                     ELADAHETFTREFGLSLVGGCCGTTPEHISAVVERVRAKPLAPRKPRRDPG"
     gene            1101..1949
                     /locus_tag="VH419_01910"
     CDS             1101..1949
                     /locus_tag="VH419_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017935366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HEY7042402.1"
                     /translation="MISIEAERQLDDAIVIAAFEGWNDAGEAATDAVNHLLVSWKATL
                     LATVDPEDYYDFQVNRPHVGFDTNGRRELSWRSTSFWHCSTTPNDRQVILVTGIEPNL
                     RWKSFCAEALDYVTSIGATTFVTLGALLADVPHTRPVPITGISSDPELAELYGLEPSR
                     YTGPTGIVGVIQEASHRRGLKTLSYWAAVPHYVAQPPAPKATLALLGQLEDLVGVPID
                     MRGLPEEARAWERGVDELASDDSDVAEYVRSLEEEQDTADLPEATGDAIAREFERYLK
                     RRNEER"
     gene            complement(2124..3395)
                     /gene="mshC"
                     /locus_tag="VH419_01915"
     CDS             complement(2124..3395)
                     /gene="mshC"
                     /locus_tag="VH419_01915"
                     /EC_number="6.3.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR03447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010125 - mycothiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--1-D-myo-inosityl
                     2-amino-2-deoxy-alpha-D-glucopyranoside ligase"
                     /protein_id="HEY7042403.1"
                     /translation="MRAWPSPSSPDVTFRELGPAPELALYDTRSGEVRTRRPDGPARL
                     YVCGITPYDATHMGHAATYTAFDLAIRAWNAAGHAVRYVQNVTDVDDPLLERAQATGE
                     DWVALAERETELFREDMAALSVIPPDVYAGAVESIPLIVAFIEELLARGAAYDVDGDL
                     YQAVSVDPHYGEVSRLPRDTMRALFAERGGDPDRPGKRDPLDPLLWQRQREGEPGWES
                     PFGTGRPGWHVECTAIARHHLGTPLDVQGGGTDLVFPHHEMSASHAHVADPDRPFAHA
                     YSHGGMVGYDGHKMSKSRGNLVLVSALRADGVDPRVIRLALHSQHYRNDWDWTDALVD
                     DAAKRLSRWVDAVAADEHEVGETLAAAREVRAALADDLDAPRALAVVDDWAARRSAGP
                     GHEAGHLVAGRPVGHVMADVIHASLGIPLLP"
     gene            complement(3437..4282)
                     /locus_tag="VH419_01920"
     CDS             complement(3437..4282)
                     /locus_tag="VH419_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020384913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO1664 family protein"
                     /protein_id="HEY7042404.1"
                     /translation="MDSDALQTDEPAVLDGATIDQILTHGDLELDGRLVSASNASFLG
                     RVTLDAISLGCVYKPVAGERPLWDFPEGTLAGREVAARLVSEAGGFGVVPPTVLRDGR
                     FGTGMCQLWVHVDPDMDGAAGSGLVDVVPTDFDEPGWIAVLEAEDHRDRPVLLVHADD
                     DRLRDMAVLDVVINNADRKGGHVLVDLQGSVWGCDHGVCFHEEPKLRTVLWGWAGERL
                     RERDVAMLTELTDRCRTGLDDVLEQHVSRTEVDAMAYRIDRLLSARRMPRPGDRWPVI
                     PWPAF"
     gene            complement(4257..4937)
                     /locus_tag="VH419_01925"
     CDS             complement(4257..4937)
                     /locus_tag="VH419_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012921417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3090 family protein"
                     /protein_id="HEY7042405.1"
                     /translation="MSIVVHSFEQPDRFVAGTVGEPGQRTFFLQAREGSRITSVALEK
                     QQVSVLAERVDMLLDEALRRSGGDPTIPAVAPAGIGDQEPLDLPIVEEFRVGTITLSW
                     DHEDSRVVIELFPISDETVVMESFGDDSDSESDNSDSEGDDDAQRGAGDGLGDDEVDE
                     VEELEVEVEASEVLIVRLEPGYARAFVRRAQSVVSAGRAPCQFCGNPIDPAGHLCPRA
                     NGFRRIAD"
     gene            complement(4967..5680)
                     /locus_tag="VH419_01930"
     CDS             complement(4967..5680)
                     /locus_tag="VH419_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY7042406.1"
                     /translation="MATVLLVRHGRSTANSSGVLAGQAPGVLLDDVGKEQAQATGARI
                     APLPVRALVTSPLERCHHTAEAIAGARSDDLEIVVDDRLTECGYGDWTGHDLKQLAKD
                     PLWKAVQAHPSGVTFPGGESMRAMQARGVEAVREWDRRVTDEHGPDALWVAVSHADVI
                     KAIVADALGTHLDNFQRIVIDPGSVSVISYTPLRPFVVRYNDHGELVALIPPKRRRGR
                     KKAGSDAVVGGGAGSAAAP"
     gene            5743..6735
                     /gene="corA"
                     /locus_tag="VH419_01935"
     CDS             5743..6735
                     /gene="corA"
                     /locus_tag="VH419_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012921419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HEY7042407.1"
                     /translation="MGDVIVDCAVYRQGKRVQLDDGGTDIAAAYSWARLNEDDFVWVG
                     LFEPTQNEMDTVADAFGLHPLAVEDTVLAHQRPKLERYGDVLFMVLKTIWYVDANDAV
                     ETGEIAVFVGERYVVTVRHGEGLELHEVRQDLEQHTDVLGHGPYAVLYSVCDRVVDGY
                     EAVSAELDNDVDEIEMSVFSDYRTRDSHRIYTLKREVAEFRRAVAPLREPLNRFAQCQ
                     VAGMPQAAGAFFRDVADHALRVHEQIENIDALLSSAHDAHMAQISVQQNDDMRKISSW
                     VAIAAVPTLIAGIYGMNFEHMPELTWTYGYPAVIVLMLIICGTLFRLFKKSGWL"
     gene            complement(6821..7648)
                     /locus_tag="VH419_01940"
     CDS             complement(6821..7648)
                     /locus_tag="VH419_01940"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017935359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEY7042408.1"
                     /translation="MDLLDAIILGIVEGLTEFLPISSTGHLTIAEHMLGLPIDDPAVT
                     AFTVVIQSGAILAVILYFRRDIWAIIRAFFRGIGDPSVRGSLDYRMGWYVIVGSIPIV
                     VVGVAGESLVTGPLRNLWWVAGGLIGWSVVMVAAERVATQRRHEPDLTMRDSVIIGVV
                     QCVALIPGVSRSGATISAGLFRGLDRVTATRISFFLSIPALVGAGLKELPSALGGDIP
                     VGSTIVATVVSFVVAYATVAWLLRFVAGHPITWFVLYRVALGLLLVVLLATGTVAAT"
     gene            complement(7654..8715)
                     /locus_tag="VH419_01945"
     CDS             complement(7654..8715)
                     /locus_tag="VH419_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012921421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEY7042409.1"
                     /translation="MKLALSMGYVLGSGPANSAQDVVALAQEAERLGYSVVWTAEAYG
                     SDAATVLAYLAAKTDRIGLGAAVMQIPARTPAMTAMTAATLDLLSAGRLRLGLGVSGP
                     QVSEGWHGVRFDKPLARTREYVEIVQQALRRQTVTHDGEFFPLPLPDGPGKPLKLSVH
                     PARDHIPLYLAAVGPKNLELTGEIADGWLAIFFSPEYAAQSFEHLEAGRATAGRTGTP
                     FDVAATVPVVVGPDVETCAEPLRAYAALYVGGMGSRRQNFYNQLARRMGFEKEAEFVQ
                     DLYLDRRHREAMAAVPLEFIDQTSLIGPRERIADRLHAFSEAGVTTLSVSTFAPDLSM
                     RIATLRVVSEALATAGLAD"
     gene            8839..9807
                     /locus_tag="VH419_01950"
     CDS             8839..9807
                     /locus_tag="VH419_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY7042410.1"
                     /translation="MKHRYLGRSGLKVSRLGLGTMLWGTETDEHDAADQLRAFVDAGG
                     TLIDTANSYGGGASESLVGTLLGMVVDRDDIVLATKGGIGRPKGERIVDVSRGGLLRD
                     LDASLRRLDVDHVDLWQIHTWSDEVPLDETLTAADAAVATGRARYVGISNYSGWQTAR
                     AATHQSAAPGRATLSSTQVEYSLLQRGIEREVVPAAQALGLGVLCWAPLGRGVLTGKY
                     RTGVPADSRAASPSYERYVGEHLDARAAQIVEAVCRAAEGLDLSPTEVALAWVRDQPG
                     VTAAIVGARTSAQLTGCLTVEGVTLPEEIVDALDDVSAIELSYPEI"
     gene            complement(9792..11612)
                     /locus_tag="VH419_01955"
     CDS             complement(9792..11612)
                     /locus_tag="VH419_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017935193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEY7042411.1"
                     /translation="MPLPIEEYAVLGDTGTCALVGRDGSVDWLCLPRFDSQACFAALL
                     GSPRHGRWLIGPKDKPTSTRQYVGNSFVLETIHETESGAVRVTDVMPLGDNRADVVRR
                     IEGIRGTVVMYHEWVVRFGYGKVRPWVTRRKDEEGRDVISAIAGPDMLVLRGSRLPKA
                     SDGKHTDTFEVREGDDLVFSTTWFRSHRPIPPMLDVVGRIDETRERSEEWLATCSYTG
                     PHEDAVRRSLLVLRLLSHGGTGGIAAAPTTSLPEEFGGSRNWDYRYCWLRDASLTLEA
                     LLAAGSVDESRVWRDWLLRAIAGDPKDVQIMYAVDGARDLPERELDHLPGYADSKPVR
                     VGNGAVTQRQTDIFGEVLSALELARQHGVDETRHSWSLQRALVNELAKHWNEPDNGLW
                     EIRGPQRHFTHSRLMVWVAFDRVIKGVENHGLEGPVEEWRTIRDAVRDQILTQGYDEH
                     RGTFVQHFETTEVDASLLLIPLVGFLPGDDPRVVGTVKAVEADLLHEGLVLRYLTQTG
                     VDGIEGGEHPFLACSFWLVAALARVGRRDDAEALMERLVALTNDVGLLSEEYDPVHDR
                     MVGNFPQAFSHLALVGAAIALADTAGRTGDERTSEGQISG"
     gene            11713..11922
                     /locus_tag="VH419_01960"
     CDS             11713..11922
                     /locus_tag="VH419_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008361292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5703 family protein"
                     /protein_id="HEY7042412.1"
                     /translation="MEYEFQRFSLPRTISRSAVRRLLIDHAEYGGWELARLRLFPDGT
                     RKVELRRRIIRVRSTLSPDVVRPSL"
     gene            complement(12083..13399)
                     /locus_tag="VH419_01965"
     CDS             complement(12083..13399)
                     /locus_tag="VH419_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020384930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEY7042413.1"
                     /translation="MTQPSSRSYAPDAEVVQICRDLIRMDTTNYGDGSGSGEREAAQH
                     VAGLLDEVGIESQVFEPSKGRTSLVAHWGDPNATDRLLLHGHLDVVPAAKADWKVDPF
                     SGEEVDGCIWGRGAVDMKDFDATILSVVRARARAGELPRRAVTMAFTADEEAGSIQGA
                     HPLVKEHPEWFEGCTEAIGEVGGASATVFGRRVYLIETAEKGIAWLRLRAHGTAGHGS
                     MRNTDNAVTAVAEAVAVIGRHEWPVRLTPTMKALLEQVAAFTGHGSVGDDVDALIDKL
                     GFGSQMLAAVVRNTANPTMLDAGYKHNVVPGEATASVDGRFLPGYEDEFFETLRGLLP
                     DSVSYDVLVHDRAVETSWDGPITEAIVASVRDEDPEAVVLPSMFSGGTDAKAWSELGI
                     RCFGFTPLRLPPDLDFSALFHGVDERVPVDALEFSARVLDRFLDRA"
     gene            13511..13596
                     /locus_tag="VH419_01970"
     tRNA            13511..13596
                     /locus_tag="VH419_01970"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13545..13547,aa:Leu,seq:gag)
     gene            complement(14116..14598)
                     /locus_tag="VH419_01975"
     CDS             complement(14116..14598)
                     /locus_tag="VH419_01975"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEY7042414.1"
                     /translation="MARAGCRDRGGAASGPRSASARPVRYLLDTNVVSELRRPAAAEA
                     VRGWVRSQAVATLAISVITVMELEIGVHRVERRDPDQGEVLRRWLEQRVLTAFRDRVL
                     PVDLAVARRAAELHVPHPGPERDSLIGATALVHGLTVVTRNVRDFAAASVPLLNPWET
                     "
     gene            complement(14526..14762)
                     /locus_tag="VH419_01980"
     CDS             complement(14526..14762)
                     /locus_tag="VH419_01980"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEY7042415.1"
                     /translation="MTEISAREFNRDVSAAKRAAEVEAVVITDHGRPSHVLLSYAEYE
                     RITGTAIDLAEWLALDVEIVGEQPLDLGLRVPDL"
     gene            15002..15430
                     /locus_tag="VH419_01985"
     CDS             15002..15430
                     /locus_tag="VH419_01985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042416.1"
                     /translation="MPWLWIIAVVVLVVLLAALIWWTSGRAKPEIKGQGDFFDVHGHD
                     QQTSYGIDPFEGREESRRLNVTAVSETLCRGPIIQSQESRMANSPAAEAEWRAATDRS
                     CCRVDSSAAHEVVGSPVGVAADQVGCVRGKQDAATMRGDD"
     gene            complement(15868..16386)
                     /locus_tag="VH419_01990"
     CDS             complement(15868..16386)
                     /locus_tag="VH419_01990"
                     /inference="COORDINATES: protein motif:HMM:TIGR01382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HEY7042417.1"
                     /translation="MSLDERKVLVLAADLFEDMELLYPVFRLREEGVAVTIAGLDDEP
                     VHGKKGYGPLDVDTTVDAVAADGFDALVVPGGFAPDKLRRSAKVLDLVRGFDTAGKPI
                     AFICHAGWVPISAKILSGRRATSVGAIRDDMENAGVDWVDEATVVDGNLISARTPDDL
                     GPWMKALFAALR"
     gene            complement(16467..>16840)
                     /locus_tag="VH419_01995"
     CDS             complement(16467..>16840)
                     /locus_tag="VH419_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042418.1"
                     /translation="LLAEALAAIVHPSPWDLGEIVWWSAVAGAVPDVPGDLAKPFRLM
                     DDAMADPADDVWRQAADSWRALGCPLWVAQCLARLSDLSAAREALQILDDLGAPAVRR
                     AVVRDRQRQGLVVPDAPSPGT"
BASE COUNT         2720 a         5805 c         5673 g         2642 t
ORIGIN      
        1 gccggggtcg cggcgtggtt tgcggggggc gagcggtttg gcccggaccc gctcgacgac
       61 tgccgagatg tgctcgggcg tcgtaccgca gcagccgccc acgagggaca gaccgaactc
      121 gcgggtgaac gtctcgtggg cgtcggcgag ctcgaccggc gtgagggggt acgacgcacc
      181 ctcggcggtg agctgcggga ggccggcgtt gggcatgcac gcgagcggga ccgccgcctg
      241 ccgggacagg tagcgcaggt gctcgctcat ctcggccgga ccggtcgcgc agttgaggcc
      301 catcatgtcg atgtcgagcg gttccagggc cgtcaaagcc gccccgatct cggtcccgag
      361 cagcatggtg ccggtcgtct cgacggtgac attgacgatg agcagcggct ggtcctgccg
      421 gtcggcgtac gccccctccg caatggcgcg tttggcgccg atcaccgcgg ccttcgcctg
      481 cagcaggtcc tgggcggtct cgacgaggac agcgtccacg ccgccctcga tgagtccccg
      541 cacctgctcc tggtaggcgt cgcgcaacgt cgcgaacgtc acgtgctgga gcgtgggcag
      601 cttggtaccc ggcccgaccg agccgagcac ccaccggggc cgggcggggg tggcagcggc
      661 gtccgcggcg tcgcgggcga tctgcgcgcc ggtgcgggcg agctcccgga tgcgaggcac
      721 gatgtcgtac tcgctgaggt tcgcgaagtt tgcgccgaag gtgttggttc cgacgcagtc
      781 cgcgcccgct gcgaagtatg cgtcgtggat cacccgtacg acgtccggac gagacacgtt
      841 gaggatctcg ttgcatccct cgagcccctc gaagtcgtcg agggtcaggt cctgctgctg
      901 cagcatcgtg cccatcgccc cgtccgccac gacgacacgc tcggcgacgg cagcgcgaag
      961 tgacgcgaca cggttggaca cttgacctcc acggatgcgg ttgaatgtcg ccagccaggt
     1021 tatcccgggc gccggtcccg accgcggtgt atctcaggtc acgagtgtcg gcgccctggt
     1081 gacgcccgta ggcttgctag gtgatcagca tcgaggcgga gcggcagctc gacgacgcca
     1141 tcgtcatcgc ggcgttcgag ggttggaacg acgcgggcga ggcggcgacc gacgccgtca
     1201 accatctgct ggtgtcgtgg aaggccacgt tgctggcgac ggtcgacccc gaggactact
     1261 acgacttcca ggtgaaccgt ccgcacgtcg gcttcgacac caacggccgg cgcgagctga
     1321 gctggcgctc gaccagcttc tggcactgct cgacgacgcc gaacgatcgc caggtcatcc
     1381 tcgtcaccgg catcgagccg aaccttcgct ggaagtcgtt ctgcgccgag gcgctcgact
     1441 acgtcacgtc catcggcgcg acgacgttcg tgaccctcgg ggcgctgctc gccgacgtgc
     1501 cccacaccag gcccgtcccg atcaccggga tctcgtccga ccccgagctc gcggagctgt
     1561 acggcttgga gccgtcgcgg tacaccgggc cgaccggcat cgtcggcgtg atccaggagg
     1621 cgtcccaccg gcgcggcctc aagacgctgt cgtactgggc cgcggtgccg cactacgtcg
     1681 cccagccgcc cgcgccgaag gccacgctgg cgctgctcgg ccagctcgaa gacctggtcg
     1741 gcgtaccgat cgacatgcgc ggcctgccgg aagaggcgcg agcctgggag cgaggggtgg
     1801 acgagctcgc ctcggacgac agcgacgtcg cggagtacgt ccggtccttg gaggaggagc
     1861 aggacaccgc tgacctgccg gaggcgaccg gtgacgcgat cgcccgcgag ttcgagcgct
     1921 acctcaaacg ccgcaacgaa gagcgctgac aacaccccgg ccatcccccg gcgaggtggc
     1981 actcgcggtc cgacacgccg ccaattacca acaagacctg ccacctcgcc gggggtcccc
     2041 ggcgaggtgg caggttttgt tggggacacg ccggttgagc gaacggcttt gccacctcgc
     2101 cgtggactcg cggtaggcga gcgtcagggc aggaggggta tgccgaggga ggcgtggatg
     2161 acgtcggcca tcacgtggcc aacgggccgt ccggccacca agtggccggc ttcgtgacca
     2221 ggcccggctg agcgacgagc cgcccagtcg tcgacgaccg ccagcgcgcg gggcgcgtcc
     2281 aggtcgtcgg ccagcgcggc ccggacctcc cgcgcggcgg ccaacgtctc accaacctcg
     2341 tgctcgtccg ccgcaaccgc atcgacccac ctgctcagcc gcttggcggc gtcgtcgacc
     2401 agcgcgtccg tccagtccca gtcgttgcgg taatgctggc tgtgcagcgc gagtcggatg
     2461 acacgcgggt cgacaccgtc ggcgcgcaac gcggacacca gcacgaggtt gccacgcgac
     2521 ttggacatct tgtgcccgtc gtagccgacc attccgccgt gcgagtaggc gtgcgcgaac
     2581 ggacgatccg ggtccgccac gtgggcgtgg ctggccgaca tctcgtgatg cgggaagacc
     2641 aggtcggtgc ctccgccctg gacgtcgagc ggcgtaccca gatggtgacg ggcgatcgcc
     2701 gtgcactcga catgccatcc aggtcgcccg gtgccgaacg gggactccca gccaggttcg
     2761 ccctcgcgct gccgctgcca gagcagggga tcgagcggat cgcgcttgcc gggacgatcg
     2821 ggatcgccgc cgcgttcggc gaagagcgcc cgcatggtgt cgcgcggcag cctcgacacc
     2881 tcgccgtagt gtgggtcgac ggagacggcc tggtaaaggt cgccgtcgac gtcgtacgcg
     2941 gcgccgcgcg ccaggagctc ctcgatgaaa gcaacgatga gcggtatcga ctccaccgcc
     3001 ccggcgtaca cgtccggcgg gatgaccgac agcgcagcca tgtcctcgcg gaacagctcg
     3061 gtctcacgct cggccagcgc gacccagtcc tcaccggtcg cctgggcacg ttcgagcaac
     3121 gggtcgtcca cgtcggtgac gttctgcacg tagcggacgg cgtgtcccgc agcgttccag
     3181 gcgcggatcg ccagatcgaa ggccgtgtac gtcgccgcgt gccccatgtg ggtggcgtcg
     3241 tacggcgtga tgccgcagac gtacaaccgg gcgggcccgt cgggacgcct cgttcggacc
     3301 tcgcccgagc gggtgtcgta caacgccagc tccggtgccg gcccgagctc ccggaaggtc
     3361 acgtccgggg acgacggtga cggccaggca cgcatgaggg aagcgtaacg acgacgaggc
     3421 ggcaggtcag gtccggtcag aaggcgggcc aggggatgac cggccaacga tcgcccggcc
     3481 tgggcatcct ccgcgccgac agcagccggt cgatccgata agccatcgcg tcgacctcgg
     3541 tgcggctcac gtgctgctcc agcacgtcgt ccagtccggt tcgacaacgg tccgtcagct
     3601 cggtcagcat cgcgacgtcg cgctcgcgca gtcgctcgcc ggcccagccc cagaggacgg
     3661 tgcgcagttt cggctcctcg tggaagcaga caccgtggtc gcacccccag accgaccctt
     3721 gcaagtccac gagcacgtgc ccacccttgc ggtcggcgtt gttgatgacc acgtcgagga
     3781 cggccatgtc gcgcagccgg tcgtcgtcgg cgtgcacgag cagcacgggc cggtcgcggt
     3841 ggtcctccgc ctccagcacc gcgatccagc cgggctcgtc gaagtcggtc ggcacgacgt
     3901 cgaccaggcc tgatccggcc gctccgtcca tgtcgggatc gacgtggacc cacagctgac
     3961 acatcccggt cccgaacctg ccgtctcgca gcacggtcgg cggtacgacg ccgaacccac
     4021 ccgcctccga caccagccgc gcggccacct cgcggccggc caacgtcccc tccgggaagt
     4081 cccacagcgg ccgctccccc gcgaccggct tgtagacgca accaagggag atcgcgtcca
     4141 acgtgacgcg acccaggaag ctggcgttcg acgcggagac gagacggcca tcgagctcga
     4201 gatcgccgtg cgtcaggatc tggtcgatgg tcgccccgtc gaggaccgcc ggctcgtcag
     4261 tctgcaatgc gtcggaatcc attggctcgt ggacacaagt gccccgccgg atcgatcgga
     4321 ttgccgcaga actggcacgg cgcacgcccg gccgacacga ccgactgggc ccgacgcacg
     4381 aacgcgcggg catagccggg ttcgagcctg acgatcagga cctcgctcgc ctccacctcg
     4441 acctcgagct cctcgacctc gtcgacctcg tcatcgccga gaccgtcgcc ggcgcctcgc
     4501 tgggcgtcgt cgtcaccttc gctgtctgag ttgtcgctct cgctgtccga gtcgtcgccg
     4561 aaggactcca tgacgacggt ctcgtcggag atcgggaaga gctcgatgac cacgcgactg
     4621 tcctcgtgat cccaactgag cgtgatcgtt ccgacgcgga actcctccac gatcggcagg
     4681 tccaacggtt cctgatcccc gatcccggcc ggagcgaccg ctgggatcgt cgggtcgccg
     4741 ccactgcgtc gcagggcctc gtcgagcagc atgtccaccc gctcggcgag tactgacacc
     4801 tgctgcttct ccagcgcgac gctggtgatc ctgctgccct cccgagcctg caggaagaac
     4861 gtccgctgtc caggctcacc gaccgtaccg gccacgaaac gatccggttg ctcgaagctg
     4921 tggacgacga tcgacatgcg cccagcctag tgtcccgcaa ccgaggtcaa ggcgcggccg
     4981 ccgagcctgc gccgccgccc acgacagcat cactcccggc cttcttgcgc cctcgacgac
     5041 gcttcggggg gatcagggcc accagttcgc cgtggtcgtt gtaccgcacg acgaacggcc
     5101 gcaacggggt gtacgagatc accgagacag agcccggatc gatcacgatg cgctggaagt
     5161 tgtcgagatg agtgcccagg gcgtcggcca cgatcgcctt gatgacgtcg gcatgactca
     5221 ccgcgaccca caaggcgtcc ggcccgtgtt cgtcggtcac ccgacggtcc cactcacgca
     5281 ccgcttcgac cccacgcgcc tgcatggccc gcatggactc cccgccgggg aaggtgacgc
     5341 cgctcggatg cgcctgcacc gccttccaga gcgggtcctt cgcgagctgc ttgaggtcgt
     5401 gaccggtcca gtcgccgtac ccgcattccg tcagccggtc gtcgacgacg atctcgaggt
     5461 cgtcgctgcg cgcgccggcg atcgcctctg cggtgtggtg acatcgttcg agcggactcg
     5521 tgacgagcgc ccggacgggc agcggggcga tgcgcgcgcc ggtcgcctgc gcctgctcct
     5581 tgccgacgtc gtcgaggagt acgccggggg cctgccccgc caggacgccg ctgctgttcg
     5641 ccgtggaccg gccgtgccgc acgagcagga ccgtcgccat ggggtcagcg taggccgtgc
     5701 ccagcagcgt ggcgcggcgc ggcggccacg gcacggggga caatgggaga cgtgatcgtc
     5761 gactgcgctg tgtaccgcca gggcaagcgg gtccagttgg acgacggcgg tacggacatc
     5821 gccgctgcct actcgtgggc tcgactcaac gaggacgact tcgtctgggt cggcctgttc
     5881 gaaccgacgc agaacgagat ggacaccgtc gccgacgcgt tcgggctgca cccgctggcg
     5941 gtcgaggaca cggtgctggc gcaccagcgg cccaagctgg agcggtacgg cgacgtcctg
     6001 ttcatggtgc tgaagacgat ctggtacgtc gacgccaacg acgcggtcga gaccggggag
     6061 atcgccgtgt tcgtcggcga acggtacgtc gtcaccgttc ggcacggcga agggctcgag
     6121 ctgcacgagg ttcggcagga cctggagcag cacaccgacg tgctcggaca tggaccgtac
     6181 gccgttctgt actccgtctg cgaccgcgtt gtcgacggct acgaagcggt cagcgccgag
     6241 ctcgacaacg atgtcgacga gatcgagatg tcggtgttct ccgactaccg cacccgtgac
     6301 tcgcaccgga tctacacact gaagcgcgag gtggccgagt tccgccgggc ggtggcgccg
     6361 cttcgggagc cactgaaccg gttcgcgcaa tgccaggtcg cggggatgcc acaggccgcc
     6421 ggcgccttct tccgtgacgt cgccgaccac gcgctgcgtg tgcacgagca gatcgagaac
     6481 atcgacgcct tgctctcgag cgcgcacgac gcccacatgg cgcagatctc ggtgcagcag
     6541 aacgacgaca tgcgcaagat ctcgtcatgg gtggcgatcg ccgcggtccc gacgttgatc
     6601 gccggcatct acggcatgaa cttcgagcac atgcccgagc tcacctggac gtacgggtac
     6661 ccggcggtga tcgtcttgat gctcatcatc tgcggcaccc tgttccgcct cttcaagaag
     6721 tccggctggc tgtaggccct ccgcgaattg cggggacttc cgggtttttc ggccccagaa
     6781 tcccggaacc tcccgcaatt cgcgaggccc caaggcgatg tcaggtggcg gcgacggtgc
     6841 cggtggcgag gagtacgacg aggaggagtc cgagcgcgac ccggtacagc acgaaccacg
     6901 tgatcggatg cccggccacg aaccgcagca accaggcgac ggtcgcgtag gccacgacga
     6961 agctgacgac cgtggcgacg attgtcgagc ccaccgggat gtccccaccg agcgcgctgg
     7021 gcagttcctt cagtcccgcc ccgacaagcg ccgggatcga caggaagaac gaaatgcggg
     7081 tcgcggtcac ccggtcgaga ccacggaaca ggcccgccga gatcgtcgcg ccggaacgcg
     7141 agaccccagg aatcagcgcg acgcactgga cgactccgat gatcaccgag tcgcgcatcg
     7201 tcagatcggg ttcgtggcgc cgctgtgtgg cgacccgctc cgccgcgacc atgacgacgc
     7261 tccagccgat cagaccgccc gccacccacc acaggttccg cagcggaccg gtcaccaggc
     7321 tctcgccggc cacccccacc acgacgatcg ggatcgagcc gacgatgacg taccagccca
     7381 tccggtagtc gagcgaaccg cgcaccgacg ggtcgccgat gccgcggaag aaggctcgga
     7441 tgatggccca gatgtccctg cggaagtaga ggatcaccgc caggatcgcg ccggactgga
     7501 tcacgacggt gaaggccgtg accgccgggt cgtcgatggg gaggccgagc atgtgctcgg
     7561 cgatcgtcag atgtccggtg ctcgatatcg ggaggaactc ggtcagccct tcgacgatgc
     7621 cgaggatgat cgcgtccagc agatccacgg gcgtcagtct gctagtccgg cagtggccag
     7681 tgcctccgaa acgacgcgaa gtgtggcgat tctcatggaa agatcagggg cgaacgtcga
     7741 cacggacagg gtcgtgacgc cggcctcgga gaacgcgtgc aggcggtctg cgatccgctc
     7801 gcgtggtccg atgagcgacg tctggtcgat gaactccagc ggtacggcgg ccatcgcctc
     7861 gcggtgccgg cggtcgaggt acaggtcctg gacgaactcc gcctccttct cgaagcccat
     7921 ccgccgggcc agctggttgt agaagttctg ccggcgggag cccatgcctc cgacgtagag
     7981 agcggcgtag gcacgaaggg gttcagcgca cgtctcgaca tcgggtccga cgacgacagg
     8041 gacggtcgcc gcgacgtcga acggcgtacc ggtgcgaccc gccgtcgcgc gcccggcctc
     8101 gaggtgctcg aacgactggg cggcgtactc cggcgagaag aagatcgcca accatccgtc
     8161 ggcgatctct ccggtgagct cgaggttctt cggtcccacg gcggcaaggt agagcgggat
     8221 gtggtcgcgt gcggggtgga cggacagctt gagcggcttg cccgggccgt ccgggagcgg
     8281 cagcgggaag aactcgccgt cgtgtgtcac ggtctggcgt cgcagcgcct gttgcacgat
     8341 ctcgacgtac tcgcgggtgc gtgccaacgg cttgtcgaac cgtacgccgt gccagccttc
     8401 ggacacctgg ggaccggaga cgcccagccc gagacggagc cggccggcgc tgagcaggtc
     8461 gagcgtcgcc gccgtcatcg cggtcatcgc cggcgtccgg gccgggatct gcatgacggc
     8521 tgcaccgagc ccgatccggt cggtcttcgc ggcaaggtac gccagcacgg tggcagcgtc
     8581 ggagccgtac gcctccgcag tccacaccac ggagtacccg aggcgttcgg cctcctgcgc
     8641 cagcgccacg acgtcctgcg ccgagttggc ggggccgctg cccagcacgt agcccatgct
     8701 cagcgcgagt ttcacccgcg caggctacac ggtgcgctga tggacgtgat gcggcgaaga
     8761 agccgcgcga cgtgctcacc acgccaccgt cgtcgatctg cagatggccg gtgctcctgg
     8821 tgcactagcc tctgcggcgt gaagcaccgc tatctgggac gctccgggct gaaggtgtct
     8881 cgcctgggcc tcggcaccat gctgtggggc accgagaccg acgagcacga cgcggccgac
     8941 cagcttcggg cgttcgtgga cgccggtggg acgctcatcg acaccgcgaa cagctatgga
     9001 ggcggagcca gcgagtcact ggtggggacg ctgctgggca tggtcgtcga tcgcgacgac
     9061 atcgtgctgg ccacgaaggg cgggatcggc cgaccgaagg gcgaacggat cgtcgacgtc
     9121 tcccgcggcg ggctgctgcg ggacctcgac gcctcgctgc gacgcctcga cgtcgaccac
     9181 gtcgatctct ggcagatcca cacctggtcg gacgaggttc cgctcgacga gacgttgacc
     9241 gccgctgatg cggccgtcgc gaccggccgg gcgcggtacg tcggcatctc gaactactcc
     9301 gggtggcaga ccgcgcgggc cgccacccac cagtcggcag cgccaggccg ggcgacgctc
     9361 tcctcgacac aggtcgagta ctcgctgttg cagcgaggca tcgagcgcga ggtcgtgccg
     9421 gctgcccagg cgctgggcct cggtgtcctc tgctgggcgc cactcggtcg cggtgtcctc
     9481 accggcaagt accggaccgg tgttccggcc gactcccggg cggcgtcgcc gtcgtacgag
     9541 cggtacgtcg gcgagcacct cgacgcgcgc gcggcgcaga tcgtggaggc cgtctgccgg
     9601 gcagcggagg gcctcgacct gtcgccgacc gaggtggcac tcgcctgggt ccgtgaccag
     9661 cccggggtca cggccgcgat cgtcggcgca cgcacaagtg cgcagctcac gggctgcctc
     9721 accgtcgaag gggtcacgct ccccgaggag atcgtcgacg ccctcgacga cgtgtcggcg
     9781 atcgagctga gctaccccga gatctgacct tcgctcgtcc gttcgtcacc ggtccgtccc
     9841 gcggtgtcgg caagggcgat ggccgcaccc acgagcgcga gatgcgagaa cgcctgcggg
     9901 aagttgccga ccatccgatc gtggacgggg tcgtactcct ccgacagcag gcccacgtcg
     9961 ttcgtcagcg cgaccaaccg ttccatcagc gcctccgcat cgtcgcgtcg gccgactcgc
    10021 gcgagcgccg ccaccaacca gaacgagcag gcgaggaacg ggtgctcgcc gccctcgatc
    10081 ccgtcgacac cggtctgcgt caggtagcgc agcacgagtc cttcgtgcag gagatccgcc
    10141 tcgaccgcct tgaccgtgcc gaccacacgg gggtcgtcgc cgggcaggaa tcccaccagc
    10201 gggatcagca gcaacgatgc gtcgacctcg gttgtctcga agtgctggac gaacgtgcca
    10261 cgatgctcgt cgtacccctg agtcaggatc tggtcgcgaa cggcgtccct gatcgtgcgc
    10321 cactcctcga ccggtccctc gagcccgtgg ttctcgacgc ccttgatcac ccggtcgaag
    10381 gcgacccaga ccatgaggcg cgagtgggtg aagtggcgct gcggcccacg gatctcccac
    10441 aaaccgttgt ccggctcgtt ccagtgcttg gcaagctcgt tgacgagggc ccgctgcagc
    10501 gaccacgagt ggcgggtctc gtcgacgccg tgctggcgag ccagctcgag agcggacagc
    10561 acctcgccga agatgtcggt ctgccgctgg gtcacggcgc cgttgccgac ccggaccggt
    10621 ttggagtcgg cgtagcccgg aaggtggtcg agctcgcgct ccggaaggtc acgtgcgccg
    10681 tcgacggcgt acatgatctg gacatccttc gggtcgccgg cgatggcccg cagcagccag
    10741 tcacgccaca cccggctctc gtcgaccgag ccggccgcga ggagtgcctc gagcgtgagt
    10801 gaggcgtcac gcagccagca gtaccggtag tcccagttac gggagccgcc gaactcctcc
    10861 gggagtgacg tggtcggcgc ggccgcgatg ccaccggtgc cgccgtggct cagcaggcgc
    10921 agcacgagaa gcgaccggcg gaccgcatcc tcgtgcggac ctgtatagct acacgtcgcg
    10981 agccattcct ccgagcgctc tctggtctcg tcgatgcgac cgacgacgtc gagcatcggc
    11041 gggatggggc gatgcgaacg gaaccaggtc gtcgagaaga cgaggtcgtc accctcgcgc
    11101 acctcgaacg tgtcggtgtg cttgccgtcg gacgcctttg gcagccggct gccgcgcagg
    11161 acgagcatgt ccggtccggc gatcgccgag atgacgtcgc gcccctcttc gtccttgcgt
    11221 cgggtcaccc acggtcgcac cttgccgtag ccgaacctga cgacccactc gtggtacatg
    11281 acgacagtgc cgcggatgcc ttcgatgcgt cgtacgacgt cggcccggtt gtcgccgagc
    11341 ggcatcacgt cggtcacccg caccgcgccg gactcggtct cgtggatggt ctcgagcacg
    11401 aacgagttgc cgacgtactg ccgggtgctg gtcggcttgt ccttcggtcc gatcagccat
    11461 cggccgtgcc tgggactgcc gagcagggct gcgaagcagg cctgggagtc gaaccggggt
    11521 agacacaacc agtcgacaga gccgtctcgt ccgacgaggg cgcaggtccc ggtgtcgccg
    11581 aggacggcgt actcctcgat gggcaacggc atggctcgaa ggttagccgg gacacgcccg
    11641 cctcggagtc gcggcgcgac acttcacccg tcgggccacg agccggtgct ccgtcgtgtc
    11701 acgatggtgg cgatggagta cgagttccag cgcttctcgt tgccacggac gatctcgcgg
    11761 tcggcggtcc gtcgactgct catcgaccac gcggagtacg gcggctggga gttggcgcgg
    11821 ctgcggctct tcccggacgg gactcgcaag gtcgagctcc gccgacgcat catccgggtc
    11881 cggtcgacgc tctcccccga cgtcgtccgc ccctccctct gacgccccgc gttctcccgc
    11941 gcgtccgttc cactgtcctg tggtgggttt gcgatgttta cagcccactt cccgtcttca
    12001 catcgcaggt ctggagtgtc aacacgccac ttgggctgta aacatcgcaa accttcgccg
    12061 ccggaccggc accgaaggcg gatcaggctc ggtcgaggaa gcggtcgagg acccgcgcgc
    12121 tgaactccaa cgcatcgacg gggacccgct cgtcaacccc gtggaacagg gcgctgaagt
    12181 cgaggtccgg aggcagccga agtggggtga atccgaagca ccggataccg agctcgctcc
    12241 acgccttcgc atccgtgccg cccgagaaca tcgagggaag cacgacagcc tccggatcct
    12301 cgtctcgcac ggacgcaacg atcgcctcgg tgatcggtcc gtcccatgac gtctccaccg
    12361 cccggtcgtg gaccagcacg tcgtacgaca cgctgtcggg cagcaggccc cgcagcgtct
    12421 cgaagaactc gtcctcgtat ccgggcagga agcgcccgtc gacgctggcg gtcgcctcac
    12481 cgggtacgac gttgtgcttg taccccgcgt cgagcatggt cgggttggcg gtgttgcgaa
    12541 ccacggcggc gagcatctgc gagccgaacc ccaacttgtc gatcaaggcg tcgacgtcgt
    12601 ctccgacgga accgtgaccg gtgaacgccg cgacctgctc gagcaaagcc ttcatcgtcg
    12661 gagtgagccg taccggccac tcatgacggc cgatcaccgc caccgcctca gcaacggcgg
    12721 tgacggcgtt gtcggtgttg cgcatcgagc catgcccggc ggtcccgtgc gctctcagcc
    12781 gcagccaggc gatgcccttc tccgcggtct cgatgaggta cacccgacga ccgaacacgg
    12841 tggcggacgc accgccgacc tcaccgatcg cctcggtgca cccctcgaac cactccggat
    12901 gctctttgac aagcggatgc gctccttgga tgctgccggc ctcctcgtcg gcggtgaacg
    12961 ccatcgtcac cgcacgcctc ggcagctcac cggcccgagc ccgcgcccgc actaccgaga
    13021 ggatcgtcgc gtcgaagtcc ttcatgtcga cagcgcctcg cccccagata cagccgtcga
    13081 cctcctcccc cgagaacggg tcgaccttcc agtcggcctt ggcggcgggt acgacgtcga
    13141 ggtgtccgtg cagcagcaac cggtcggtgg cgttgggatc gccccagtgg gcaacgagcg
    13201 acgtacgacc cttcgacggc tcgaagacct gcgactcgat gcccacctcg tcgaggaggc
    13261 cggcgacgtg ttgggccgcc tctcgctcgc ctgacccgga accgtccccg tagttcgtgg
    13321 tgtccatccg gatgaggtca cggcagatct ggacgacttc tgcgtcgggt gcgtacgacc
    13381 gggagctggg ctgcgtcata gcgcccatct tgcacccctg gcggccggca gcgattgcag
    13441 gcaatcggcc ggactttgct agggttggtg gtcgcctacc gacgtcgtat gacgtccggt
    13501 tggcacccac gtccgggtgg cggaataggc agacgcgcta gcttgaggtg ctagtgcccg
    13561 tattagggca tgggggttca agtcccccct cggacacaac agctccaggt ctctgacctg
    13621 gggctttgtc gattccgggc ctcgtctcgt gcttggggtg tagcacgacc cggaggtcga
    13681 tcgagcactc cgtcatccga tccggtggtg tcgcacccac ctgcgcacgt gctcgggctt
    13741 cgtcaacacg tcgaagacga gcgatcggag catcgaactc tcgagtgatg accacctcga
    13801 gcttgtccgg gaatgtgatg actgcagaac catgccgatc agctgtcact ggcatctcct
    13861 tgtcgttgca ggttctcgag gtagtcctcg agccgatcca gtcggtcgtt gatcgtccgc
    13921 tcgtacttgg cgacccagtc gtgcaagggc cgaagatgct cgggggccag gcggtagatt
    13981 cctgcgtccc gcccgcgtca tcgtgggcgg aatggggtac ctcggccacg ggcgagtccc
    14041 cgcagctgcg cgtggcgctg ccagtcacac cactcggaat tgttcgggcg gagggtcatg
    14101 ggtcggcggc cgccgtcacg tctcccaagg attgagcagg ggaacgctgg cggccgcgaa
    14161 gtccctgacg ttccgtgtca cgaccgtcag gccgtgaaca agcgctgtcg ccccgatcag
    14221 cgaatcccgc tccggtcccg gatgtgggac gtgcaactcc gccgcccgcc gggcgaccgc
    14281 cagatcaacg ggcaggactc ggtcccgaaa ggccgtcagg acacgctgct cgagccaccg
    14341 gcgcaaaacc tcaccctgat ctggatcacg tcgctccacc cggtgaaccc cgatctcgag
    14401 ctccatcacc gtgatgacgc tgatcgccag cgtggcgact gcctgcgatc ggacccaccc
    14461 gcgcaccgcc tcagccgctg ccggtcggcg cagctcactc acgacattcg tgtcgagcag
    14521 gtacctcaca ggtcgggcac tcgcagaccg aggtccagag gctgctcccc cacgatctcg
    14581 acatccagcg cgagccactc cgccagatcg atcgcggtac cggtgatccg ctcgtactcg
    14641 gcatacgaca acaagacgtg cgaagggcgc ccatggtccg tgatcacgac cgcctccacc
    14701 tcggccgccc gcttggccgc actgacgtca cggttgaatt cgcgtgcgct gatctcggtc
    14761 accgtccgca cctcctgtag gtagtgtacc tacatcgcat ccacccttcg tcagtgcaag
    14821 ctcactggaa aggacccatg tgttcgacgg cggtgtcctc ggacactcac tcaccaggca
    14881 gaatgccgct tcacacacgc agatccaccc gcgggcacgc tgtcggaagc aagcgtcctc
    14941 atcaagaaca cgtgggcggc ttccttgcct gcgggcgtac ggtgcctacg gaggacgagt
    15001 catgccgtgg ttgtggatca ttgcggtcgt cgtacttgtt gtgctgcttg ccgcgctgat
    15061 ctggtggacg agtggtcggg ccaaacctga gatcaagggt caaggggact tcttcgacgt
    15121 acacgggcac gatcagcaga cgagttatgg catcgatccg ttcgaagggc gcgaagagtc
    15181 caggagactg aacgtcactg cggtttcgga gacactgtgc cgtggtccga tcatccagtc
    15241 acaggaaagc aggatggcca actcacctgc cgctgaggcg gaatggcggg ccgcgaccga
    15301 ccgatcctgt tgccgggtcg actcatcggc tgcgcacgaa gtcgtcggtt cgcctgttgg
    15361 tgtcgccgcg gaccaggttg gttgcgtaag aggcaaacag gatgcggcga ccatgcgggg
    15421 cgatgattga cgcgaaactg tctccgtttc ctgttccacc accccgtgct actgacacga
    15481 tccgagtgaa gtgatgggcg cggttgcgga cgaacacgtc aagtttgtgg ttggtgtcgc
    15541 cgcggaccag tgaagcatcg ctggagcgga aagtcaccag gttctttcgt cccccatgca
    15601 ttgactgctg ctgcggcgtc aacccggtga gccgaccgag tggtatgtgc cgtccatgca
    15661 gcaaatgctg cggtccgaac gtcgccgccc aactgtgcta gcacggcacc aacgagtgcc
    15721 acagccaccg ttgttgatcg tgcttgcatg tccctccttg ccagcgtctg ttggcgcgga
    15781 atggtccact aacgcactca catgccactg gtcggcgctt gcgttggtcg agcgtcgggg
    15841 acaagccggg tcaatcgccc cgccgcacta ccgcagggcg gcgaacaatg ccttcatcca
    15901 tggcccgaga tcgtccggcg tacgcgcgga gatgaggttg ccgtccacga cggtggcttc
    15961 gtcgacccag tcgacgccgg cgttctccat gtcgtctcga atggccccga cgctcgtggc
    16021 gcgccgcccg gagaggatct tcgccgagat cggcacccag ccggcgtgac agatgaaggc
    16081 gatcggtttg ccggccgtgt cgaagccgcg gacaaggtcg agcaccttcg ccgagcggcg
    16141 cagcttgtcc ggcgcgaacc cgccgggcac gacgagcgcg tcgaacccgt cggcggccac
    16201 cgcgtcgacg gtggtgtcga cgtcgagtgg cccgtagccc ttcttgccgt gcaccggctc
    16261 gtcgtcgagt ccggcgatcg tcaccgccac gccttcctcg cgcagccgga aaaccgggta
    16321 cagcagctcc atgtcctcga acaggtcggc ggcgaggacg agcactttgc gttcatcgag
    16381 cgacatgcct caaaccctac gcgcgccacc cggaggcggg cagccgccgt cgccccaagg
    16441 ccggccactc atcggcatgt ggccagtcag gttccgggcg agggagcgtc cggcacaacc
    16501 aggccctgcc gctgccgatc gcggaccacc gcacgtcgta cggcgggggc gccgaggtcg
    16561 tcgaggatct gcagcgcctc gcgggcggcc gagagatccg aaaggcgggc caggcactgg
    16621 gcaacccaca aggggcagcc gagcgctcgc cagctgtcag cggcctgtcg ccagacgtca
    16681 tcggccgggt ccgccatcgc atcgtccatc aaccggaacg gcttcgcgag atcgcctggg
    16741 acgtcaggaa ccgcgccggc cactgccgac caccacacga tctcgccgag gtcccacggc
    16801 gacgggtgca cgatggcggc gagtgcttcg gcgagaaggc
//
LOCUS       DATBFZ010000067         2437 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_2996832, whole genome shotgun sequence.
ACCESSION   DATBFZ010000067 DATBFZ010000000
VERSION     DATBFZ010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2996832"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..592)
                     /locus_tag="VH419_02000"
     CDS             complement(<1..592)
                     /locus_tag="VH419_02000"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HEY7042419.1"
                     /translation="MSETPPPTSTDQAPEDMTRRNFIEHVGFMSGTALLYAACKPSEP
                     KKSEVTVGPVVESSHRSLTNDEYDTVAAAVERICPRDEEPGAIDLGVPEYIDRALATP
                     TLAHVRDDFARGTEVLMRRAQGRHQKPFAALTPDLQDALLTEFKDSPAASGEQHYWTL
                     LIGLTMEGLFGDPAHGGNRGGRGWELIGFGNGRRGCA"
     gene            706..2181
                     /locus_tag="VH419_02005"
     CDS             706..2181
                     /locus_tag="VH419_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042420.1"
                     /translation="MRPTSMALAVGAMVALAFGGCSQKFGELIYDDSLLCGDLNQACA
                     PDGGRFDLHIVDRTSYVVATPSVPTFDMVIKPNSTSVGFAYVVVSPRQDSGVIAMFPD
                     AGIVDYDIMFREFDVDAGVASPPQLVQTVQRFFGVSVAYQVNGFPAVAYLGGPPDFTE
                     TFWFQNDAAIAYNQGGRWVEQVAATDSSPPMASPPSPPSDNGKIVGFYPALIFEGNNA
                     VLAYRDGHFGQSAGTGDWNSADLEMAVGGPTTWTPVALLQSGDSKRAYGAHNRIIYGA
                     KTLSSGLRAAAVVSDRAGTAPDTFGADIEFSEQQDDGTWKSCGAFQPLTIADTPSILG
                     ANGASMLPNTQAGPSIDYRDNFGYAIAVTNRSQNGVFFTACAANTDCTTPSGWDTLDP
                     VFQRGSGGWYAAVAISPDGDPSVAYYICSKQPNRNENTCTDDHYLAVANRGLSQVWSD
                     HEVVVDTGSVFQTRMVFIGTRRVIGYRNGASGVLQIAVEKP"
     gene            2178..>2437
                     /locus_tag="VH419_02010"
     CDS             2178..>2437
                     /locus_tag="VH419_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042421.1"
                     /translation="MTRSSLVLLVAGAIVVSVACGKGDNSLDGSLSEVFDLDVSTVHV
                     LRNDDALVVSYEHNAGRDIDLVLRFTLALDQLPLQTGRAMNIA"
BASE COUNT          398 a          834 c          820 g          385 t
ORIGIN      
        1 cggcgcaccc ccgccgcccg ttgccgaagc cgatgagctc ccagccacgg ccaccgcggt
       61 tgcctccgtg cgccggatct ccgaacaggc cctccatcgt gagcccgatg aggagcgtcc
      121 agtagtgctg ctcgccggag gcggcgggcg agtccttgaa ctcggtgagc agcgcatcct
      181 ggaggtcggg ggtgagcgcg gcgaagggct tctggtgtcg accctgagcc cggcgcatca
      241 gcacctcggt gccgcgagca aagtcgtcac ggacgtgcgc gagggtcggc gtggccagtg
      301 cccggtcgat gtactcgggg acgccgaggt cgatggcacc cggctcctcg tcgcgcgggc
      361 agatgcgctc cacggccgcc gccaccgtgt cgtactcgtc gttggtgagc gagcggtggg
      421 agctctcgac caccggtccg accgtcacct cggatttctt cggctcggac ggtttgcacg
      481 ccgcgtagag caacgccgtt ccggacatga agccgacgtg ctcgatgaaa tttctgcgcg
      541 tcatgtcctc cggagcctga tccgtggacg tgggtggagg tgtctccgac atgcggagga
      601 gtgtcctccg agaaatcgac gggccgcaag acgatgcatg caaggggatc cgcgatgccg
      661 ctggtagaat gcgagccgga tccgggggtg aaagtgaagg aagggatgcg tcccacatcg
      721 atggcgctgg ccgtcggcgc catggtcgcg ctcgcgttcg gcggctgcag ccagaaattc
      781 ggcgagctca tctacgacga cagcttgctc tgcggtgatc tgaaccaggc ctgcgcaccg
      841 gacggcgggc ggttcgatct ccacatcgtg gatcgcacga gctacgtcgt ggccacgccc
      901 tcggtcccga ccttcgacat ggtgatcaag ccgaacagca ccagcgtcgg cttcgcctac
      961 gtcgtcgtga gcccgcggca ggactcgggc gtcatcgcca tgtttcccga cgcgggcatc
     1021 gtcgactacg acatcatgtt tcgcgagttc gacgtggacg ccggagtcgc gtccccgccg
     1081 cagctggtgc agacggtcca gcgcttcttc ggcgtgagcg tcgcctacca ggtcaacggc
     1141 ttccccgcgg tggcgtacct cggcggacca ccggacttca ccgagacctt ctggttccag
     1201 aacgacgcgg ccatcgccta caaccagggc ggccgctggg tggagcaggt ggcggccacc
     1261 gactccagcc cacccatggc gagcccaccg tcacctccca gcgacaacgg gaagatcgtc
     1321 ggcttctacc cggcgctcat cttcgagggg aacaacgcgg tgctcgccta ccgcgacggc
     1381 cacttcggtc agtccgcagg caccggcgac tggaactcgg cggatctgga gatggcggtc
     1441 ggcggcccca cgacgtggac cccggtcgcg ctgctgcagt cgggcgacag caagcgcgcc
     1501 tacggcgccc acaaccgcat catctacggg gcgaagacgc tgagttccgg gctgcgggcc
     1561 gctgcggtcg tgtcggatcg ggcgggcacc gccccggaca ccttcggggc ggacatcgag
     1621 ttctccgagc agcaggacga cgggacctgg aagagctgcg gcgccttcca gccgctgacc
     1681 atcgccgaca cgccgagcat cctcggtgcg aacggcgcgt cgatgctgcc caatacccag
     1741 gccggcccct ccatcgacta ccgcgacaac ttcggctacg cgatcgccgt caccaaccgc
     1801 agccagaacg gcgtgttctt caccgcctgc gccgcgaaca ccgactgcac gacgccctcg
     1861 gggtgggaca cgctcgatcc ggtgttccag cgcggaagcg gcggctggta cgcggcagtg
     1921 gccatctcgc cggatggaga cccgtcggtc gcgtactaca tctgctccaa gcagcccaac
     1981 cggaacgaga acacgtgcac cgacgaccac tacctcgcgg tggcgaaccg ggggctcagc
     2041 caggtgtgga gcgaccacga ggtggtggtg gacacgggct cggtgttcca gacccggatg
     2101 gtcttcatcg gcacccgccg ggtgatcggc taccggaacg gtgccagcgg cgttctgcag
     2161 atcgcggtcg agaagccatg actcgatcgt cgctggttct tctggtggcc ggggcgatcg
     2221 tggtctcggt cgcctgcggc aagggggaca actcgctgga cggaagcctc tccgaggtgt
     2281 tcgacctcga cgtctccacc gtccacgtgc tccgcaacga cgacgccctg gtggtgagct
     2341 acgagcacaa cgcaggccgg gacatcgacc tcgtgcttcg cttcaccctg gccctggacc
     2401 agctgccgct gcagaccggc cgcgccatga acatcgc
//
LOCUS       DATBFZ010000068         8632 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_1029338, whole genome shotgun sequence.
ACCESSION   DATBFZ010000068 DATBFZ010000000
VERSION     DATBFZ010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 8632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8632
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1029338"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..595
                     /locus_tag="VH419_02015"
     CDS             <1..595
                     /locus_tag="VH419_02015"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HEY7042422.1"
                     /translation="AYHTRMGQALCELIEHLSADAMPQHGVGNAQIVIILDEQAMRHG
                     TGPVVLDTGDQVSVAEARRLACNACIMPMVLNGRSRILDLGMGQRLFDRNQRLALAHR
                     DGGCVFPGCERPPAWCDGRSRATESPGPAQQATCLVLRRGSSNGCLVCGFHHRLIHKG
                     DWSIQTATDGVVEVIPPDWVDLKRQPLRHQRFKPRPG"
     gene            complement(598..1494)
                     /locus_tag="VH419_02020"
     CDS             complement(598..1494)
                     /locus_tag="VH419_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020389069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042423.1"
                     /translation="MTNDPLAAARAFLDTEGRLIERRLAAVLFDDADRSGVIDAVRAY
                     RNPDGGFGHGLEPDKRCPASLPLDVECALDILLAAGASEDSIGSADRHEIDGLVSGAC
                     DWLASVAGPDGAVSLCFPVIEAYPRAEHVSDWTYEPGLNPTAGLTGRLHRMHVTHPWL
                     DRATAWCWNRLEQGFDEDAHTLGEVLVFLANVPDRDRAEVLSAQVGDWLSGARWFRAD
                     PGDPEYGVTPLHLARTPDSPWRRLFDDATIEGHLDRMLRDQQPDGGWPITWEPPSMAS
                     TLDWRGIETLRALRTLRAYDRL"
     gene            1582..2766
                     /locus_tag="VH419_02025"
     CDS             1582..2766
                     /locus_tag="VH419_02025"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HEY7042424.1"
                     /translation="MSTPRRFDVTERRARLAVRHHLATEAQETGTAAASLIGLHATDP
                     ASVYLSAWARVDGVKHDDIDDALYEARTIVKHMAMRRTVWALATDLLPVVQAAASDAI
                     AVNERKRLARDLEKSGVTTNGPAWVRRAEKAAMNALATMGPSFGRELSSAVPVLRTKV
                     RVGSANRSQEIGVVTRITTILSASGQATRARAGGTWVNRQPRWVRMQDWVPRVTEDPP
                     TSAAAARADLARHWLRAFGPATVDDLKWWAGWTVAQTKAALEAIGAIGVDLVGAEPGV
                     ALPDDLEPVPAPDPWVSLLPSLDPTTMAWKHRDWYLGSHGGRLFDPYGNAGPTVWSDG
                     RVIGGWGQRPDGEVVVQLLEDVGADVTASVTAEAERLTGWLAGVQVRPSFPTPLQREL
                     SA"
     gene            complement(2849..4633)
                     /locus_tag="VH419_02030"
     CDS             complement(2849..4633)
                     /locus_tag="VH419_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042425.1"
                     /translation="MTQRAASAMRRRAGYGLATMACLGAAALVNASPPALGVHATDPC
                     PAAYPEDELTVDQSVNGLTVTQGTEPSEFTGKVLGVLDDGIAPGVDMIMMDLTSPTID
                     KVGIWQGMSGSPVYAEDGRLIGAVAYGLAFGPSTVAGITPFGDMQALLDQAPASAKTR
                     TTLADAKRAVRVDISSKQARQLARHTDVSAKNLDQGLERLPMPFVVSGNAHRLNQAAH
                     KLGMKGVRAFQGHTAAANSTPSVIEAGGNLAATVSYGDLTSGGVGTATAVCDNGQHVL
                     AFGHPMLWTGPTSLTMHGADAVYIQDDPTSAGFKVANLTAPAGTIVADRLTGLLGLDN
                     TLPDTTRVTAHVEKIGGGSRDGTTIISVPDAVPEISALHLLTDEDRVFDQLTPGSAQV
                     TWTVRGKRSDGTPFHYTRTNRYASEYDLTYETIFESYDQLYRLMHNKFDDVTITHISY
                     TSTLNPDFKAYKLGSVERRVNGQWEKLSRTTVRVQGGSTMHLRAHLRPLGDGTAKVVP
                     LTLHVPQVKRSTYGFLQVTGGAWVFERPTGSSFGQFLHSLSHATHNNDVVGQVSVRTN
                     GSNHVSKGTGSVDQVVQGQLGTSLRIIH"
     gene            5047..5202
                     /locus_tag="VH419_02035"
     CDS             5047..5202
                     /locus_tag="VH419_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042426.1"
                     /translation="MSSRTTNPGLPGGRTRRSLLHWPAPDTLVLLLLLVIAIIAALLA
                     GFALGAR"
     gene            complement(5310..5591)
                     /locus_tag="VH419_02040"
     CDS             complement(5310..5591)
                     /locus_tag="VH419_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HEY7042427.1"
                     /translation="MCRNIKVLHNFEPPADESEIHAAATQYVRKVAGMTKPSAANQHA
                     FDEAIAAVTAATRTLLDELVTHAPKRDREVELAKARARHEKRFGVPATR"
     gene            5716..6516
                     /locus_tag="VH419_02045"
     CDS             5716..6516
                     /locus_tag="VH419_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042428.1"
                     /translation="MQRQRKATFFVLAAIAAATGPIGAGSASPAAGSLSTSAPPPTSA
                     TTKTRVEPVAPVHPAPSHFTHGRVDNAWYPLRPGTRYVFKGAEDGVRERDVVLVTYQT
                     EVIDGVTCRAVHDRLIIHGNVRERTTDWFAQTKRGTVWYFGERTAELNPRGRVVSREG
                     SWRSGRHGAEAGIFMPRHPRVGQSFLQENSPGVAVDRFRVLSLHAHATAPLISSHHAL
                     LTKETTRLEPGVVDHKLYVRDIGQVSEQTVRGGDEALHLVSLRHVPRG"
     gene            complement(6524..7195)
                     /locus_tag="VH419_02050"
     CDS             complement(6524..7195)
                     /locus_tag="VH419_02050"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEY7042429.1"
                     /translation="MRRFSAAVAVVLPVLVLVTPAAPARVVDSSPGPETGPAARLGLG
                     LGSPLRRTDPSSNAHHAKPRRFWVRDKHRYSSPWYAGVHRKMIAFGCTPAPYYDPDPR
                     CAHHRGFHHGLDIAMPCGTKLFAGYRTRVVDPTSSGALGSAYGRWAFRLRSARLHKDF
                     VIGHVRRVFVEPGDLVRRGELIARASDAGAPDGCHLHFEVRPVRAGYQSAVRPGPYLR
                     LDRDR"
     gene            7306..7458
                     /locus_tag="VH419_02055"
     CDS             7306..7458
                     /locus_tag="VH419_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPGG-motif small membrane protein"
                     /protein_id="HEY7042430.1"
                     /translation="MYFLLWILAVILVIWGIVMLFSGSILWGIVLIIVGLLVGPGGVS
                     IFHRRA"
     gene            complement(7502..8476)
                     /locus_tag="VH419_02060"
     CDS             complement(7502..8476)
                     /locus_tag="VH419_02060"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEY7042431.1"
                     /translation="MRLTAVASVVGVVLAGCSSNGSDGDVPPRKTAPVPSTTSGDCGD
                     FRIAYDPSKGYEASAFVVGTLAEDQLGCTVHYVKTTSRAAWRLVADGDADVYMDAYGS
                     PGLQAALTRKGGPVVRLGPNGFKGGVDVLAPAFMGNLGLRTYRDLPDTTSIGWGSVTP
                     AITTVPELLPLAQSFVQFQDLDYAVRDYSQVGTGSGMGDLLQQPRADDENHRPNLYLV
                     AAPLGLIGDGPGRSSVEIPESAADDCVPNRVSTLCSLANFRYQKIVNSAFARSGSPAY
                     NLVYRYRIGPEEADNLLEIVELSGYDVQSADVASWINTHPNVWRRWLD"
BASE COUNT         1370 a         2930 c         2952 g         1380 t
ORIGIN      
        1 ggcctatcac acccggatgg ggcaggcgtt gtgtgagctg atcgaacacc tgtccgccga
       61 cgcgatgcca cagcacgggg tgggcaacgc gcagatcgtc atcatcctcg acgagcaagc
      121 gatgcggcac gggaccggtc cagtggtgtt ggacaccggc gaccaggtct cggtcgccga
      181 ggcgcgccgg ttggcgtgca acgcctgcat catgccgatg gtgctgaacg gtcggtcccg
      241 catcctcgac ctcggcatgg ggcaacggct gttcgaccgc aaccaacgcc tcgcgctcgc
      301 ccaccgcgac ggtggctgtg tgttccccgg ctgcgaacgc cctccggcct ggtgtgatgg
      361 acggtcccga gcgacggagt cgccgggacc ggctcaacaa gccacctgcc tcgtcctacg
      421 tcggggcagt agcaacgggt gcctggtgtg cgggttccac caccggctca tccacaaagg
      481 cgactggtcg atacagacgg ccaccgacgg ggtcgtcgaa gtcatcccac cagactgggt
      541 cgacctgaaa cgccaaccac tccgacacca acggttcaaa ccccggccag gctgacctca
      601 cagccggtca taggcgcgca gcgtacgcag ggcacgaagc gtctcgatcc cacgccagtc
      661 cagggtggag gccatgctcg gtggttccca cgtgatcggc cagccgccgt cgggctgctg
      721 gtccctgagc atccgatcca ggtgaccctc gatcgtggcg tcgtcgaaga gtcgtcgcca
      781 cgggctgtcc ggcgtccgcg cgaggtgcag cggagtcacg ccgtactccg ggtctcccgg
      841 gtccgctctg aaccatcgag cacctgacaa ccagtcacca acctgcgcgc tcagcacctc
      901 tgcgcgatcc cggtcgggaa cgttggcgag gaacaccaac acctcaccga gggtgtgagc
      961 gtcctcgtcg aatccttgct cgagacggtt ccaacaccat gcggtggccc ggtcaagcca
     1021 gggatgtgtg acgtgcatcc gatgcagccg gccggtgagt cccgcggtcg ggttcagtcc
     1081 tggttcgtac gtccagtcgg acacgtgctc agcccgcggg tacgcctcga tcaccggaaa
     1141 gcacaacgac accgccccat cggggcccgc gaccgatgcg agccagtcac aagcaccgct
     1201 cacgagtccg tcgatctcgt gccgatcggc cgagccgatg ctgtcctccg aggcaccggc
     1261 cgccagcagg atgtcgagcg cacactcgac gtcgagaggg aggctggccg ggcaccgctt
     1321 atccggttcg agtccatgtc cgaacccacc gtccgggttg cggtaggccc gcaccgcatc
     1381 gatgactcca gagcgatcgg cgtcgtcgaa cagcaccgcg gccagtcgtc gctcgatcaa
     1441 ccgtccctcg gtgtcgagga atgctcgggc ggcagcgaga gggtcattcg tcacccacgc
     1501 agtctggcac gcacgatgcc gcctcggtcc cggccgaccg cgcccatggc tggcaccccg
     1561 catcggctga aaagatgagg catgagtact ccgcgccgat tcgacgtcac cgaacgtcgt
     1621 gcgcggttgg cggtgcggca ccacctggcg accgaggcac aggagaccgg caccgctgca
     1681 gcgtcactga tcggattgca cgccaccgac ccggcctcgg tctacctgtc cgcctgggcg
     1741 cgcgtcgacg gcgtcaagca cgacgacatc gacgatgcac tgtacgaggc ccgcaccatc
     1801 gtcaagcaca tggccatgcg gcgtaccgtc tgggcgcttg ccacggatct gctgccggtc
     1861 gtccaggcgg ccgcgagcga tgcgatcgct gtcaacgagc gcaagcgact ggctcgggac
     1921 ctggagaagt cgggcgtgac gacgaacgga ccggcgtggg tacgccgcgc cgaaaaggcg
     1981 gcgatgaatg cgctggcaac catgggaccg tcgttcggcc gcgagctgtc gtcagcggtg
     2041 ccggtgctcc ggacgaaggt ccgggtcggc agcgccaacc ggtcgcagga gataggtgtg
     2101 gtcacgagga tcaccacgat cctctccgcc tccgggcagg cgacgcgggc gcgcgcgggc
     2161 ggcacgtggg tcaaccggca gccgcgctgg gtgcggatgc aggactgggt tcctcgagtc
     2221 accgaagacc cgccgacgtc ggccgccgcg gcgcgcgccg atctggcgag gcactggttg
     2281 cgcgcgttcg ggcccgcaac cgtcgatgat ctgaagtggt gggccggctg gaccgtcgcg
     2341 cagacgaagg ccgcgctcga agccatcggg gcgatcggtg tcgacctggt cggcgccgag
     2401 ccaggtgtcg cgctgccaga cgacctcgag cctgtcccgg ctcccgaccc gtgggtatca
     2461 ctgttgccct cgctcgatcc cacgacgatg gcctggaagc accgggactg gtatctcgga
     2521 tcacacggcg gtcggttgtt cgacccgtac ggcaatgctg gccccaccgt ctggtccgac
     2581 ggccgtgtca tcggtggctg gggtcaacgg ccggacggcg aggtggtcgt ccagctgctc
     2641 gaggacgtcg gcgctgacgt gaccgcctca gtgacggccg aggcggagcg cctcaccggc
     2701 tggcttgccg gcgtacaggt tcgtcccagc tttcccacgc cgctgcaacg cgagctctcg
     2761 gcgtgagccg ctggtcggac gaggccagac gaggccgccc ccgtgtcacg ggacggcctc
     2821 gcctgttggt gtcagtaccg ctgctgcgtc agtggatgat gcgcagcgag gtgccgagct
     2881 gaccctgaac cacctggtcg accgagccgg tgcccttgga cacatggttc gagccgttgg
     2941 tgcgcacgct cacctggccc acgacgtcgt tgttgtgcgt cgcgtgggaa aggctgtgca
     3001 ggaactgacc gaacgacgat cccgtggggc gttcgaagac ccacgcgcca cccgtgacct
     3061 gcaggaagcc gtacgtcgag cgcttgacct ggggcacgtg cagcgtcaac ggcacgacct
     3121 tcgccgtacc gtcaccgagc gggcgcaggt gggcacgcag gtgcatcgtc gagccaccct
     3181 gcacgcgcac cgtcgtgcgc gacaacttct cccattgccc gttcacacgg cgttcgacgg
     3241 agcccagctt gtacgccttg aagtccgggt tgagcgtcga cgtgtagctg atgtgggtga
     3301 tcgtcacgtc gtcgaacttg ttgtgcatca ggcggtacag ctggtcgtag gactcgaaga
     3361 tcgtctcgta ggtgaggtcg tactccgagg catacctgtt ggtgcgggtg tagtggaacg
     3421 gcgtcccgtc agaacgcttg ccgcgcaccg tccaggtgac ctgcgcggat cccggtgtga
     3481 gctggtcgaa gacccggtcc tcgtcggtca gcaggtgcag cgccgagatc tccggcacgg
     3541 cgtcgggaac actgatgatc gtcgtcccgt cacgggagcc tcccccgatc ttctcgacgt
     3601 gcgcggtgac gcgggtcgtg tccggcaagg tgttgtcgag tccgagcagg ccggtgagcc
     3661 gatcggcgac gatcgtgccg gccggtgcgg tcaggttggc gaccttgaac cccgcagacg
     3721 tcgggtcgtc ctggatgtag acggcgtcgg cgccgtgcat cgtgagcgac gtcggcccgg
     3781 tccacagcat cgggtgaccg aacgccagca cgtgctggcc gttgtcgcac accgccgtgg
     3841 cggttccgac gccgccactg gtgaggtccc cgtaggacac ggtcgcggcg aggttgccac
     3901 ccgcctcgat caccgacggc gtgctgttcg ctgcggccgt gtggccctgg aacgcccgga
     3961 cgcccttcat gcccagcttg tgcgcggcct ggttgagccg gtgcgcattc ccggagacga
     4021 cgaacggcat cggcagccgc tcgagacctt ggtcgagatt cttcgcggag acgtcggtgt
     4081 gccgcgccag ctgccgcgcc tgcttcgacg agatgtcgac ccgaaccgcg cgcttggcgt
     4141 cggcgagcgt ggtgcgggtc ttcgcgctgg cgggcgcctg gtcgagcagc gcctgcatgt
     4201 cgccgaacgg tgtgatgccg gcgaccgtcg acgggccgaa ggcgaggccg taggcgacag
     4261 cgccgatgag ccgaccgtcc tcggcgtaca ccggcgagcc ggacatgccc tgccagatgc
     4321 cgaccttgtc gatggtcggt gacgtcaggt ccatcatgat catgtcgacg ccgggcgcga
     4381 tgccgtcgtc gaggacaccg agaaccttgc cggtgaactc cgacggctcg gtgccttgcg
     4441 tgacggtcaa cccgttgacc gactggtcga ctgtgagctc gtcttccggg tacgccgccg
     4501 ggcacgggtc ggtcgcatgg acccccagtg ccggcggtga tgcgttgacg agcgctgcgg
     4561 cgcctaggca cgccatggtg gcgaggccat agccagctcg gcgtcgcatc gccgaggcag
     4621 cacgttgagt catgtttccc cctcagggtg ggccacgcca tggcgtggtg atggtgatga
     4681 gcatcgtgag cgcatgcggc cgtcgaccgc gagcgaaaac ctatgccctc tccgacgcgc
     4741 cgaagccggc tttcgttgct tcgacacgcg gatttgtcca tggggacttc gtggaagagg
     4801 gtcagccgcc gtcggctgat accgagccgc cgacgcatac ggtcacaaca cggcaacgaa
     4861 ggcagcgggg ccgagtggct tccgcgaccg gctcgaacgg gtgaggccgc ctggtcattt
     4921 cggacgatgt ttgcccggag tgcgtgtcaa cggcgaccgg caggtccatg gtggtcgcag
     4981 aatcgtgtac aggatctcgg cctatcgcat gacacgcttg acgccacgtg tcggagggga
     5041 cacggcatga gcagcaggac gaccaacccg ggcctaccgg ggggccggac ccgacgaagc
     5101 ctgctgcact ggccggcacc tgacaccttg gtcctgctgc ttctgctcgt catcgcgatc
     5161 atcgcggcac tgctggccgg ctttgccctc ggcgcccgtt gacgcctccc gtgggtcccc
     5221 gcgaattgcg gtgaacctca gcccgcaagg gggccggaga cgtgcgcttt cccgcaattc
     5281 gcgggagcac ggcagtgcgg gacggatgat cagcgtgtgg cgggtacgcc gaagcgcttc
     5341 tcgtgccgtg cgcgggcctt cgccagctcg acctcccggt cacgcttcgg cgcgtgcgtg
     5401 acgagctcgt ccagcagcgt ccgggtggcc gcagtcaccg ctgcgatcgc ctcgtcgaag
     5461 gcgtgctggt tcgccgccga cggcttcgtc atccccgcga ccttgcgcac gtactgtgtc
     5521 gcggccgcat ggatctctga ctcgtcggcg ggtggctcga agttgtggag caccttgatg
     5581 ttcctgcaca tgtcctcaac tctaggcgag tggttccgag gggtgccggt gaagcgagcg
     5641 actcagcgtc cccacagcag gtacgccgca tgctcgtcct gaacgcaggc gaacacctga
     5701 ccgcgaggag gagacatgca gcgacaacga aaggccacgt tcttcgtgct cgcggcgatc
     5761 gccgcggcga caggcccgat cggcgcaggc tcggcctcac ccgcggcagg atcgctctct
     5821 acctcagcac caccacccac ctcagcaacg acgaagacca gggttgagcc ggtggcgccg
     5881 gtccacccgg caccctcgca tttcacgcac gggcgcgtcg acaacgcctg gtacccgctt
     5941 cgcccaggaa cgcggtacgt cttcaagggg gccgaggacg gggtcaggga acgcgacgtc
     6001 gtgctggtca cctaccagac cgaggtgatc gacggcgtca cgtgccgtgc cgtccacgac
     6061 cggctgatca tccatgggaa cgtgcgcgag cggacaaccg actggttcgc gcagaccaaa
     6121 cggggcaccg tgtggtactt cggcgagcgc accgccgagc tgaacccgcg gggacgggtc
     6181 gtcagtcgag aggggtcctg gcggtccggc cggcatggag ccgaggcagg catcttcatg
     6241 ccgcggcacc cgcgcgtcgg gcagtccttc ctgcaggaga actcccccgg tgtcgctgtc
     6301 gaccgcttcc gcgtcctgtc actgcacgcc cacgcgaccg cacccttgat ctcctcgcac
     6361 cacgccctgc tgacgaagga gacgacgcgg ctcgagccgg gcgtcgtcga ccacaagctg
     6421 tacgtgcggg acatcggtca ggtcagcgag cagacggtgc gcggcggcga cgaggcattg
     6481 cacctggtct cgctcaggca cgtcccgcgg ggctgacagc ctgtcaacgg tctcgatcca
     6541 accgcagata tgggccggga cgtacggcgc tctggtaccc ggcacggacc ggccgtactt
     6601 cgaagtgcag gtgacaaccg tccggcgcgc ccgcgtccga tgcgcgcgcg atgagctcgc
     6661 cgcgtcggac caggtctccc ggctcgacga acacccggcg gacatgcccg atcacgaagt
     6721 ccttgtggag ccgcgcactc cggagccgaa aggcccaacg tccgtacgcc gagccgagcg
     6781 ctcccgacga cgtgggatcg accactctcg tccggtagcc ggcgaacagc ttggtgccgc
     6841 acggcatcgc gatgtccagg ccatggtgga agccccggtg gtgggcgcag cgtggatcgg
     6901 ggtcgtagta cggcgccggg gtgcagccga aggcgatcat cttgcgatga acgccggcgt
     6961 accacgggga gctgtaacga tgcttgtcgc ggacccagaa ccgccgtggt ttggcgtggt
     7021 gggcgttcga ggacggatcc gtgcggcgca gagggctgcc gaggccgagt cccagcctgg
     7081 cggccggacc ggtttccggc ccgggagacg agtccacgac cctcgccggg gcggccggtg
     7141 tgacgagcac gagcaccggc agcacgaccg cgacagcggc cgagaacctg cgcatttccg
     7201 acctcctggt cgctcacccg agtgtcgcac accaactccg ccctccggcg tgcgacgttt
     7261 aggcacgcgg tggcagggaa catcgaagcg cgaaggaggc acccgatgta ctttctgctc
     7321 tggatcctcg ccgtcatcct ggtgatctgg gggatcgtga tgctcttcag cggcagcatc
     7381 ctgtggggca tcgtgctcat catcgtcggc ctgctcgtcg gccccggtgg cgtgagcatc
     7441 ttccaccgac gcgcctaggc gcacgccaca ccggtgctgg ttcgaccttg ccaacccagg
     7501 ttcagtcgag ccagcgccgc cacacgttcg ggtgcgtgtt gatccacgac gcgacgtccg
     7561 cgctctggac gtcgtacccg gacagctcga cgatctcgag caggttgtcc gcctcctccg
     7621 gcccgatccg ataccggtag accaggttgt acgccgggct gcccgacctc gcgaaggcac
     7681 tgttcacgat cttctggtag cggaagttcg ccagggagca cagcgtcgac acccggttgg
     7741 gaacacagtc gtcagcagcc gactcgggga tctcgaccga actgcgtccg gggccgtcac
     7801 cgatcaggcc gagaggcgct gccaccaggt acaggttcgg tcggtggttc tcgtcgtcgg
     7861 cgcggggctg ctgcagcaga tcccccatgc cgctgccggt cccgacctgg ctgtagtcgc
     7921 gcacggcgta gtcgaggtcc tggaactgga cgaacgactg ggcgagcggc agcagctctg
     7981 ggacggtcgt gatcgccggt gtcacggagc cccatccgat cgaggtcgtg tcgggaaggt
     8041 cgcggtaggt gcggagcccc aggttcccca tgaaggccgg tgcgagaacg tctaccccgc
     8101 ctttgaaccc attcggtccg agccggacga cggggccacc ctttcgggtc agggcggctt
     8161 gcaggccggg gctgccgtac gcgtccatgt agacgtcggc gtcgccgtcg gccaccagcc
     8221 gccatgccgc ccgcgaggtc gtcttcacgt agtggacggt gcagccgagc tggtcctcgg
     8281 caagcgttcc gacgacgaag gcgctcgcct cgtagccctt gctggggtcg taggcgatcc
     8341 ggaaatcgcc gcagtcgccg gacgtcgtac tcggaacggg cgcggtcttg cgcggcggga
     8401 catcaccgtc ggagccgttg ctcgagcacc ctgccagcac gacaccgacg acgctcgcga
     8461 cggccgtgag cctcaccaag ccaagccgcg gcgaccacgt acgtcggcac ccgacagcag
     8521 ccatctcgct cgctttcccc gcccggcgcc attgccggga caccgcagcg gaccacaata
     8581 gcgccgcagc atcgcctaac cgccggctcg caccagcgtc tccggcatcc gc
//
LOCUS       DATBFZ010000069         5720 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_375618, whole genome shotgun sequence.
ACCESSION   DATBFZ010000069 DATBFZ010000000
VERSION     DATBFZ010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 5720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5720
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375618"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="VH419_02065"
     CDS             <1..531
                     /locus_tag="VH419_02065"
                     /inference="COORDINATES: protein motif:HMM:NF024770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEY7042432.1"
                     /translation="DPVPGMTEMASEIARLGHRRVGAIFGPRNTSTGLLRETALREAL
                     EGHGITIGAQLTYRGPFDFETGHSGAELLLQRQPRPTVIVCGNDVVALGALNAAKELG
                     VEVPGDVSIVGFDDLPPASWPLVQLTTVAFDLDTMARTAARLLVDRIEDQENLPYEHA
                     IFTSRLVRRRTLAAST"
     gene            625..1605
                     /locus_tag="VH419_02070"
     CDS             625..1605
                     /locus_tag="VH419_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020419253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEY7042433.1"
                     /translation="MPLDPIAYKPYADPDDFIREVTDLIWVDRAIGFIRENYEPDSIV
                     HGAFGTVVGRDEVLEGSLMRISDTPDRIAQAEDVVWEARGDDAFLSSHLVLSVDPHDD
                     VVSRTIANCLYRRGRMVEEWVVRDSLASALQRGLDPDEVARGKTFRGYVGSLTQPAPT
                     DVIACGDSGPRPDEFRPEVEMVLDFIQRVWNDRELEKVEDFWIRDLVLHTVGNKIVIR
                     PEGYRRALLKLIRPFPAGQFEVRDVQTNFNERYAGLRIAVLWKLTGRYNGTADFGPVT
                     DSPIDLLGVSQFLVQHGRIVREVRVYDEIALRTQIASRRGDEPFVGANIY"
     gene            1659..2822
                     /locus_tag="VH419_02075"
     CDS             1659..2822
                     /locus_tag="VH419_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042434.1"
                     /translation="MTDVLIEAQELQRRTIRRSDWVSCNAAFIDCRTPGSDKKENYAF
                     IGPGVSQNANQFVNLTELHGYNLGAAGMPNGVSNNLHLHFTAEVFINFGGVFRLRWGV
                     DGKQGEYVSTDGDVISVPTWIFRGFTNEGPDDGILLTVLGQDVTGGIIWGPSVLREAE
                     SHGLYLTADSRLIDTVAGDELPADIPLIKPMKQRYIDELTPYTVEEMRSRVIQPGDRA
                     YSTRSFLCTGLPGGNAELALGIGYGMSEDRRAVPRVHEPHSFNLAWLRAVEGEGMLRH
                     RHSETQVLVVKSGVWEVTLNEGDQAETVKLAAQDAFSVPAGSWRSMRLIEPGSNAATD
                     GTGELLVINGGDGRTRLEWAPEVVDAAFNAGWMLDPNGYLAPTAVMITATEDD"
     gene            3163..3642
                     /locus_tag="VH419_02080"
     CDS             3163..3642
                     /locus_tag="VH419_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEY7042435.1"
                     /translation="MADTQKAAKSTTAIKKTSGSAFTEEERAAMKERAQEVKAANGRT
                     GGKSKAADESALLEKIAEMKEPDRALAEQVHSVITDAAPELSPTLWYGMPAYAKDGKV
                     VCFFQPAAKFKARYSTLGFNDPANLDDGAMWPTAFALTKLTAAEEKKIGALVRRAVR"
     gene            complement(3683..4156)
                     /locus_tag="VH419_02085"
     CDS             complement(3683..4156)
                     /locus_tag="VH419_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020576285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HEY7042436.1"
                     /translation="MGAEQRFTVTVADGGRGRALIPVPFDPDAVWGAKTRHHVGGSVN
                     GKRVRAVIESRGAEFGLLIGRAWLRDGCVAIGDQAQVVIEPEGPQRDDLADDVAAALD
                     ANPRAGEFFDSLAQFYRRGYLRWIDSTKRSPELRAERIATMVGLLEAGVKDYHDQ"
     gene            4365..4850
                     /locus_tag="VH419_02090"
     CDS             4365..4850
                     /locus_tag="VH419_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042437.1"
                     /translation="MALPSRTMRTAPAVPLRRLRASLLALAFLFTTVLAAVVAPPTAD
                     AAGVKYNSYFVTDVYISVTGGRVNGYIRITQDVGSTDFYFVTATVYVTQCRADLTVCG
                     TFAANSGGGSNIVSTSSKPAAYGHVYKTCSSFTLWDPTYPPRGRTQKWTNVCSPWSSY
                     P"
     gene            5256..5672
                     /locus_tag="VH419_02095"
     CDS             5256..5672
                     /locus_tag="VH419_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020388945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase superfamily protein"
                     /protein_id="HEY7042438.1"
                     /translation="MDLRLELVTVPVADVERAKSFYVDQVGFRVAQDHRVDDTHRFVE
                     LVPPGSPSSIALTTGYVDSKPGSLKGIQVNVDDVDAVHEALRQHSVAVSDIQQYPWGR
                     FCFFADPDGNEWSVHEPPDAAGWESSDSCARPTSAA"
BASE COUNT         1004 a         1896 c         1899 g          921 t
ORIGIN      
        1 gacccagtgc cagggatgac cgagatggcc agcgagatcg cccgcctcgg tcaccggcgc
       61 gtgggggcga tcttcggtcc ccgcaacacc agcaccggat tgctgcgcga gaccgcgctc
      121 cgcgaggccc tggaagggca cgggatcacg atcggcgcgc agctcaccta ccgtggcccc
      181 ttcgacttcg aaaccgggca ttccggcgcc gagctcttgc tgcagcgtca gccgcggccg
      241 acggtgatcg tctgcggcaa tgacgtcgtc gcactcggcg ccctgaatgc cgccaaggag
      301 ctcggggtcg aggtgccggg ggatgtctcg attgtcggat tcgacgacct gccgccggcg
      361 tcctggccgt tggtgcagct caccacagtc gcgttcgacc tcgacaccat ggctcggacg
      421 gccgcccgac tgctcgtcga tcggatcgaa gatcaagaga acctgccgta cgagcacgcg
      481 atcttcacct cgcggctggt gcgacgtcgg accctggccg cctcgacgta acctgtctca
      541 caccagccgg cgttgccttg cgcggatctg tgcatacgca tacactcgag atcaccggtt
      601 tccgtccacg tctgcaggag tgccatgccg ctcgatccga tcgcctacaa gccgtacgcc
      661 gatcctgacg acttcatccg agaggtgacc gatctgatct gggtggaccg ggcgatcggt
      721 ttcatccggg agaactacga gcccgactcg atcgtgcacg gcgcgttcgg caccgtggtc
      781 ggtcgggacg aggtgctcga gggctcgttg atgcggatct cggacactcc ggatcggatt
      841 gcgcaggccg aggatgtcgt ctgggaagcc cgtggagacg acgcgttcct cagctcacac
      901 ctggtgctga gtgtcgatcc gcacgacgac gtggtgagcc gcaccatcgc caactgtctg
      961 taccggcggg ggcggatggt ggaggagtgg gtcgtacgcg actcgctggc ttctgcgctg
     1021 cagcgcggtc tggatcccga cgaggtcgcg cgcggcaaga cctttcgcgg ctacgtaggc
     1081 agcctgaccc agccagcgcc caccgacgtg atcgcctgcg gcgacagcgg tccacgtccg
     1141 gacgagttcc ggccggaggt ggagatggtg ctcgacttca tccagcgcgt ctggaacgac
     1201 cgtgagctgg agaaagtcga agacttctgg attcgtgatc ttgtgctgca cacggtaggc
     1261 aacaagatcg tgatccgacc ggagggttac cgccgggcac tcctcaagct gatccgcccg
     1321 ttcccggccg gacagttcga ggttcgcgat gtccagacga atttcaacga gcgctacgcg
     1381 ggactgcgga tcgcggtttt gtggaagctc accggtcgct acaacggcac cgccgacttc
     1441 ggacccgtca cggattcgcc gatcgacttg ctcggtgtgt cccagttcct cgtccagcac
     1501 ggccggatcg tgcgggaggt ccgggtctac gacgagatcg ccctgcgcac gcagatcgcg
     1561 tcccggcgcg gcgacgaacc gttcgtcggc gccaacatct actgatcatc ctcggttcga
     1621 tccatcgagc ttgcacgcag cacagtaagg acagactcat gaccgacgta ctcatcgaag
     1681 cccaagagct gcagcgtcgt accatccgcc gcagcgactg ggtgtcgtgc aacgcggcgt
     1741 tcatcgactg ccgcacaccc ggctccgaca agaaggagaa ctacgccttc atcggacccg
     1801 gggtgtccca gaacgccaac caattcgtca acctgaccga gctgcacggc tacaacctcg
     1861 gtgccgccgg gatgccgaac ggggtctcca acaacctgca tctgcacttc accgccgagg
     1921 tcttcatcaa cttcggtgga gtcttccggc tgcgctgggg cgtcgacggc aaacagggcg
     1981 agtacgtcag cactgacggc gacgtgatca gcgtgccgac ctggatcttc cgcggcttca
     2041 ccaacgaggg ccccgatgac ggcatcttgt tgacggtgct cggccaagac gtcaccggcg
     2101 gcatcatctg gggcccgtcg gtgttgcggg aggccgagtc gcacggcctg tatctcaccg
     2161 ccgacagccg gctgatcgac accgtcgccg gtgacgagct gccggccgac atcccgctga
     2221 tcaagcccat gaagcagcgc tacatcgacg agctgacccc gtacaccgtc gaggagatgc
     2281 gttcgcgggt catccagccc ggtgaccggg cctacagcac gcgctccttc ctgtgcaccg
     2341 gcttgccggg aggcaacgcc gagctcgcgc ttggcatcgg ctacggcatg agcgaggacc
     2401 ggcgtgcggt gccgcgggtg cacgagccgc actccttcaa cctcgcctgg ctgcgagccg
     2461 tcgagggcga gggcatgctc cgacaccgcc acagcgagac gcaggtgctg gtcgtcaagt
     2521 ccggtgtctg ggaagtgacc ctcaacgaag gggaccaggc cgagacggtc aagctggcgg
     2581 cgcaggacgc cttctcggtg ccggctggca gctggcggag catgcggctg atcgagcccg
     2641 gttccaacgc ggcgaccgac ggcaccggcg agctgctggt gatcaacggc ggtgacggcc
     2701 ggacccgcct ggagtgggcg ccggaggtag tggacgccgc gttcaacgcc ggatggatgc
     2761 tcgaccccaa cggctacctc gcaccgaccg cggtgatgat caccgctacc gaggacgact
     2821 gagctgggcg ctcgagaccc ccgcttctac accgtgatca acactgatcc ggtcgggaag
     2881 gctgcacggc cgtggggcgc gcagtcggcg tcctcagctg tccgctccca cttcgcaccc
     2941 cgtacgtgta ctccgcaccc cgcacgcagt gtgcgaagtc gacctccggt gtgcgaagtg
     3001 gcccgaactg gaccgcggtc gtcgcagaca atgtgctcgg ccacgtccgt caggttgccg
     3061 acgactgggc agagcatcga tcagttttcg agaagcgcca cggcgacgga gccgcgagaa
     3121 tgacggttgc gaccgaacca gatccgcaga tgggagatca ccgtggccga cacgcagaag
     3181 gcggcgaaga gcaccaccgc gatcaagaag acgtcggggt cggcgttcac cgaagaggag
     3241 cgtgcggcca tgaaggagcg cgcccaggag gtgaaggcgg ccaacggccg aaccggaggc
     3301 aagagcaagg cggcggacga gagcgctctg ctggagaaga tcgcagagat gaaggaaccc
     3361 gatcgcgcgc tggccgagca ggtgcattcg gtcatcaccg acgccgcgcc ggagttgtcg
     3421 cccaccctct ggtacgggat gccggcctat gcgaaggacg gcaaggtcgt ctgcttcttc
     3481 cagccggcgg cgaagttcaa ggcgaggtac tcgacgttgg gcttcaacga cccggcgaac
     3541 ctcgacgacg gggccatgtg gccgaccgca ttcgcgttga cgaagctgac cgcggccgag
     3601 gagaagaaga tcggtgcgct cgtgcgaagg gcggtccgct gacctcgacc cgtcgaaacc
     3661 ccgcgacgtc ggcattgctg ccctactggt cgtggtagtc cttgaccccc gcctcgagca
     3721 gcccgaccat tgtggcgatc cgctcggccc gtagctctgg actgcgcttg gtcgagtcga
     3781 tccagcgaag gtaaccgcgc cggtagaact gggcgagaga gtcgaagaac tcgcccgcgc
     3841 gggggttcgc gtccagcgcg gcggcgacgt cgtcggcgag atcgtcccgc tgcgggccct
     3901 ccggctcgat caccacctgg gcctggtcac cgatggcgac gcagccgtcg cggagccagg
     3961 cgcggccgat caggaggccg aactccgctc cgcgcgattc gatgacggcg cgaactctct
     4021 ttccgttgac cgagccgccg acgtggtgtc tggtcttggc gccccacacc gcatccgggt
     4081 cgaacgggac ggggatcagg gcgcggcccc ggccgccgtc cgcgaccgtg accgtgaatc
     4141 gctgttcggc acccacgcga cgagtctctc atcgtccgcg tcgtcagaca gcacccggga
     4201 accgaaccgg cgacgtacct gaatgccaca ggtcctggcg acacgacagc ggctggcctg
     4261 ctctggccgc gctgagcacc gaatggtgct gcgcgcccag atcgtgctgg ccgccgccca
     4321 cggcgtagcg tcgagtacgc cgagcatcgc aaaggactgg aaccatggca ctgccgtcga
     4381 ggacgatgcg gactgctccg gcggtcccgc tgcgccggct gcgtgcgagt ctgctggcgc
     4441 tcgccttcct gttcacgaca gtgctcgcgg cagtggtggc gccgccgacg gcggacgccg
     4501 caggggtcaa gtacaacagc tacttcgtca ccgacgtgta catcagcgtc accggtggac
     4561 gcgtgaacgg ctacatccgg atcacccagg acgtcgggtc gaccgacttc tacttcgtga
     4621 cggccaccgt ctacgtcacc cagtgccggg ccgacctcac cgtgtgcggc acgttcgcgg
     4681 ccaactccgg cggcggcagc aacatcgtgt cgacttcctc gaagcccgcc gcctacggac
     4741 acgtgtacaa gacctgcagc tccttcacgc tctgggaccc gacctatcca ccccgcggac
     4801 gaacccagaa gtggacgaat gtctgctcgc cctggtcgtc gtacccgtag accgaccgac
     4861 gagcgcgcgc cgtggggcgg cggtcaacac gacggtcgta tgagccgggc cgacccatcc
     4921 tggccgagca aacaacgagg tcgaggaagg acaagcgtgc acacgaccgg tggcatgcga
     4981 ttgcggctcc cgatcccggg caccgggttc gtcacatgat caagcttcgc ccgtcaacca
     5041 ggttgggcca ccccagccgc ggacacggga acctaccgtc atgtgcgccc acacaccgcc
     5101 gttcagccag agctcggcct cgcgcttacc tccggaccac tggtcatggc aggtttccgg
     5161 ggtgttggat gcgcacggtg atagttggtg caggcctggt tggacaacga caagtcgggg
     5221 agttatcggg gagggagagc aggggaggtt tggtcatgga tcttcgcctc gagctggtga
     5281 cggtgcccgt tgccgatgtg gagcgggcca agtcgttcta tgtggaccaa gtgggtttca
     5341 gggtcgcgca agatcaccgt gtcgatgaca cgcatcgttt cgtggagttg gttccccccg
     5401 gctcgcccag ctcgattgct ctcaccacgg gctacgtcga ctccaagccc ggctcgctga
     5461 agggcatcca ggtcaatgtg gacgacgtcg atgcggtcca cgaggccttg cggcaacaca
     5521 gcgtggcggt ctccgacatc cagcagtacc catggggtcg cttctgcttc ttcgcagacc
     5581 ccgatggcaa cgaatggtca gtccacgagc cgccggacgc agcagggtgg gagagcagtg
     5641 acagctgcgc caggcccacg tcagcagcat gaccacaacc cgtccgtctt gcggacgtcg
     5701 cggcaggggg agggccggtc
//
LOCUS       DATBFZ010000070         9783 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_4946748, whole genome shotgun sequence.
ACCESSION   DATBFZ010000070 DATBFZ010000000
VERSION     DATBFZ010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 9783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9783
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4946748"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            853..1764
                     /locus_tag="VH419_02100"
     CDS             853..1764
                     /locus_tag="VH419_02100"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY7042439.1"
                     /translation="MNASSTSVEQLQFFADPGEFLAAAGDFLATDPMISTVVTTVAHR
                     AAAELSEGIAQPEQNWWLVVSDARGAVCGAGMRTAPFVPYPPYLLPMPDGAAVALARA
                     LHERGEEVLGVNGALPAVELFATELARLRNSRVTVAQHTRLHELGRLVPPQSVPGRLV
                     TATDDDVEAAMEWYAAFMGDADEQAGRPRGSSAEHIPDEPEMLRKIRAGRLWFWLDGN
                     RRRVSLVGANPPSFGVARIGPVYTPPTERGRGWASNAVAEVSRLVVADGARACLFTDQ
                     ANPTSNKIYAALGYEPVVDMANLLIVS"
     gene            complement(1805..2206)
                     /locus_tag="VH419_02105"
     CDS             complement(1805..2206)
                     /locus_tag="VH419_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020574666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HEY7042440.1"
                     /translation="MRVLLNIIWLLLCGIWMAVGYAVAGIICCILIIPIPFGIAAFRI
                     ANYALWPFGRTVVRRDTAGVGSAIGNVVWIVVAGIWLAIGHVLTGILLCLTIIGIPLG
                     VANFKMVPISLLPLGSEIVDVDDVDLDQRRL"
     gene            complement(2354..3313)
                     /locus_tag="VH419_02110"
     CDS             complement(2354..3313)
                     /locus_tag="VH419_02110"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEY7042441.1"
                     /translation="MRDGRHPTRGALLVVTGALLFVVNAGVSRVIQSAGVGSATLTTV
                     RCTGTALVLLLVVALRRELRLVVPENRQALLATVAFGITGVAMVQWLYFVAIDRLPVG
                     VALLLEFTAPVLVALFARFGYGEHVRARLWAGLACSLGGLALVTEIWSGLALDVIGVL
                     AGLGAAVSLAAYFLLGEHSVATRPPLAVLTGAFVVATVFWNIAAPLSALGSVDLGGQV
                     SLGGNLSEYDVSLWALIVWMVVLGTVAPFLAELSALRDLSATEVGLIGMVEPVGATVL
                     GWLWFEENLSAVQVVGIAIVLVGIALAQTARNVPLTAETRLMT"
     gene            3368..4456
                     /gene="hisC"
                     /locus_tag="VH419_02115"
     CDS             3368..4456
                     /gene="hisC"
                     /locus_tag="VH419_02115"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009085956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEY7042442.1"
                     /translation="MTTPRFRPALDTIPSYKAGRPPAAGQPAGQPRYKLSSNENPYPP
                     LPGVVARATEAAEAMNRYPDMGVTRLHEALAAKYSVSTDQIASGAGSVAVLYHLLQAS
                     CDDGDEVVYAWRSFEAYPIAAKLTGATSVHVPVDADARHDLPSMLEAITDKTKVVIVC
                     TPNNPTGTSVHQAELEAFLAGVPGHVLVAIDEAYHEFVTDPETPDALELARGRDNVVV
                     LRTFSKAYGLAGLRVGYAVAPAPIAEAIRKCALPFGVSSIAQAAAVASLDSEAELLER
                     VDELVKERSRVHDALRQQGWDIPDAQGNFVWFGLGDATVGFAEACDAAGVSVRPFAGD
                     GARVTIGEPEANDLLLDVAQRWHSEHTG"
     gene            4727..6541
                     /locus_tag="VH419_02120"
     CDS             4727..6541
                     /locus_tag="VH419_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013861205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY7042443.1"
                     /translation="MTAAAPPALALPSPPRQGGSRSLWRLRRYMRPYYGSLATMGSTA
                     VVGVFVSMSIPLVTRAIIDGPITDGDMSAIWPLGLAGLVLGITEAGLILVRRWVQARA
                     VLGFETAVRDDLYRHMQALPMEFHGRWQSGQLLSRVTTDLSAIRRFMGFGLLFLFINI
                     LQLTVVTLLLLHLYWPLGLVVAISAVPIIVLSKRFETKYVVVSRAVQDQQGDVATAVE
                     EGAVGFRVIKSFGRRHHIERQFRGRAQTLYNTSIEKVRLSSKFWTFLEIIPNISLVFV
                     LLLGALAVGEHEVTLGTLVAFITLMLSLVWPIEALGVILSMAQEAMTAADRVNEIFDT
                     KSAITSGTRELERPRGHLRFERVGFHFPDSDQDVLHDVDLEVLPGETLALVGATGAGK
                     TALTSLVPRLYDVTAGRITVDGVDVRELTLPCLRTVVATAFEEPTLFSMSARENITLG
                     RPDADEADIRDAIETAQASFVYDLPWGLETRIGEQGMSLSGGQRQRLALARAVLARPR
                     ILVLDDTLSALDVHTEALVEQALRHVLADATGIVVAHRASTVLLADKVALLQGGTITH
                     VGRHEELLATVPAYHDLLAGDTDHFDDLPTAQEDVVLR"
     gene            6538..8415
                     /locus_tag="VH419_02125"
     CDS             6538..8415
                     /locus_tag="VH419_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020391446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY7042444.1"
                     /translation="MTTTNERHDDVDDERADDESQNVSWRGVAAEERDEIPAKATLLL
                     QQRSRRLLKHLLAPHKKLLGLLLVAVLIENAARLSVPFLVKEGIDSGIPPILDGEGTS
                     TLYTIVGVLLLAVVIQAVTRVTFLMLAGKIGQQVLFILRRQVFSHFQRLSPAFHEEYT
                     SGRVISRQTSDIDAIYELLETGFDGLITAMLTLIGTAVLLVVLDVKLGLVALLCFPAL
                     LVLTNWFRRESAKAYRRTRETVAVVIVHFVETINGMRAVQAFRREDRNQEIFESVNDD
                     YRQANERAFRLVGLFMPGIKLIGNITIAVVLIYGARLAIEGEVTPGVLAAFLLYLRQF
                     FEPMQEISQFYNTFQSASAALEKLSGVLEEEPSVPEPKHPVPLVEAHGALHLDGVGFE
                     YVAGRPVLPDLELEVPAGMTLALVGTTGAGKTTLAKLVSRFYDPTRGRITLDGIDLRD
                     LAEDDLRKAVVMVTQENFLFNGTVADNIRFGKPDATMEEVVAAAQAIGAHDFIIGMPE
                     GYETDVANRGGRLSAGERQLVAFARAFLANPAVLILDEATSSLDVPSERLVQHALRTI
                     LADRTAIIIAHRLSTVEIADRVLVMEHGRIVEDGTPDELIAGGEGRFADLHNAWLASL
                     A"
     gene            complement(8499..>9783)
                     /locus_tag="VH419_02130"
     CDS             complement(8499..>9783)
                     /locus_tag="VH419_02130"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEY7042445.1"
                     /translation="VSSTTKVDKRPDDQAAAQDENKVDLNRVRIEIEPQAEWRQMYDE
                     AWRLMRDHYWRPDMGGTDWGAARERYAPLLSRLGSHDDLVDLIWELQGELGTSHAYVL
                     PDPDDVEPTRRQGLLGADLEYADDAWRIARVIPGEPSDRNARSPLTAPGVGARVGDAI
                     VAVDGRRPSRTRSPYALMLGTADKPVELTIAPADGGAARRAVVVPLADEMPLRYQDWV
                     NDRRRYVHEQTAGRVGYLHVPDMVSGGWAQLHRDLRTEMRRDALIVDVRANRGGHTSQ
                     LVVEKLARRVIGWDLTRGYEPESYPLDARRGPLVAVTDMYAGSDGDIVTAAIQSLGLG
                     QVVGTRTWGGVVGIDGRYRLIDGTLVTQPRYAFWFEDFGWGVENYGVDPDVEVEVAPQ
                     DRVAGNDVQLDRALALVEQQLRRTPPKVAPALPDL"
BASE COUNT         1526 a         3336 c         3369 g         1552 t
ORIGIN      
        1 tgattccaag ggaggttgcc tggctcgcgg ctccccgcat cccgcctggc tgcgttgtcg
       61 tcactccgaa taggagctcc gctatgagtc gctcctccgc cttgccagat cgggcgcgga
      121 tgagccgctc gcttcaccgg ccccttggaa tcaatcgtct ggacgagcga gacctggcag
      181 cctcgatgaa ggcggacatg cgtgggtggt ggtttgcgtg gttaaccatc cgaatgcacg
      241 gctcccgagg caaacctgag tccgcctccg ggttctgtgg cggcgacggt cgcgatgagt
      301 gggcgggtca gcccggcgtt ctgttatcag gggctgaggt ggatcggcag cgaagagcgg
      361 gagatgttgc tgcctgctga gctggccggt cggctctcgg tttgcctggg ccggggtggg
      421 tcggcattga gagcgggtga cgttcgtgcc ggctgagctg gctggccggg cttgcagacg
      481 cttgtggtcg gttggtggct ggcggttctt gggacatccg ttggtcgccg acccgcggtt
      541 tgcatggcta cccaggcaag tccacccctc ccgaggcaga cctgcctggc tacccgtccg
      601 cttggatggc tacccaggca aaccccgaga tggaaaggcg acctaccgca cgtatcggac
      661 catcgtgggt ggctgggggt ttccggggac accgcggctg accggcgtga tgtttgcgat
      721 gttcagacgg caagcccact gtgcccatcg gaggtttgcg atgtctacac gccgcttggg
      781 ctgtaaacat cgcaaacccg gcccccggaa attggcgtgc cggaccgatg cgcgcgtggc
      841 taacctgcgc ggatgaacgc atcgtcgacc tcggtcgagc agctgcagtt cttcgccgat
      901 ccgggcgagt tcctcgcggc tgccggtgac ttcctcgcca ccgatccgat gatcagcacg
      961 gtcgtgacga ccgtcgcgca ccgcgccgcg gccgagctgt cggagggaat cgctcagccc
     1021 gagcagaact ggtggctcgt ggtctccgac gccaggggtg cggtatgcgg cgccggcatg
     1081 cggactgccc cgttcgtgcc gtacccgcct tacctgctgc cgatgccgga cggcgcggca
     1141 gtcgcgctgg cgcgtgcgtt gcacgagcgc ggtgaggaag tgctcggcgt caacggggcc
     1201 ctgcctgccg tcgagctgtt cgcgaccgag ctggctcgtc tgcgaaacag tcgcgtcacc
     1261 gtggcgcagc acacccggct ccacgagctg ggccggctcg tgcccccgca gtcggtgcca
     1321 gggcggttgg tgaccgcgac ggacgacgat gtcgaagcgg cgatggagtg gtacgccgcc
     1381 ttcatgggcg acgcagacga acaggctggt cgaccacgcg gcagcagtgc cgagcacatc
     1441 cccgacgagc cggagatgct gcgcaagatc cgcgccggcc ggttgtggtt ctggctcgac
     1501 ggcaaccgac gacgtgtcag cctcgtcggg gccaacccgc cctccttcgg ggtcgcccgc
     1561 atcggacccg tctacacgcc gccgaccgag cgtggtcgcg gctgggccag caacgcggtt
     1621 gctgaggtct cccggctcgt cgtggccgat ggcgcccggg cttgcctgtt caccgaccag
     1681 gcgaacccga cgtcgaacaa gatctacgcc gcgctgggtt acgagccggt cgtcgacatg
     1741 gccaacctcc tgatcgtcag ctgacccgcc cgtccggtgc cggacgacgt gcggttgagc
     1801 ctggtcagag ccggcgctgg tccaggtcga cgtcgtcgac gtccacgatc tcgctcccca
     1861 gcggcaggag cgagatcggg accatcttga agttcgcgac gccgagcggg atcccgatga
     1921 tcgtcaggca cagcaggatt ccggtgagca cgtgaccgat cgcgagccag atgcctgcga
     1981 cgacgatcca gaccacgttg ccgatggccg atccgacgcc ggcggtgtcg cgccgtacga
     2041 cggtcctccc gaacggccag agtgcatagt tcgcgatgcg gaaggcagcg atcccgaagg
     2101 ggatcgggat gatcaagatg cagcagatga tgccggccac cgcgtacccc accgccatcc
     2161 agatgccgca gagcaacagc cagatgatgt tgagcagcac gcgcatgacg tgtcccttcg
     2221 cgacctcggc gtcgtcagga aagtctgcgc acggagatcc gaaccgcgtc aacctcgtcg
     2281 aagggcaggc gagaagggca ccgtccgtga cgaaggagcc gctcggcagg tcgggatcgg
     2341 tggaaccgct tgctcaggtc atcagccggg tctcagcggt gaggggcacg ttgcgcgcgg
     2401 tctgggcgag cgcgatgccg accagcacga tggcgatccc gaccacctgc accgccgaca
     2461 ggttctcctc gaaccacagc cagccgagca ccgtcgcgcc cacgggctcc accatcccga
     2521 tcaggccgac ctcggtcgcg ctgaggtccc gcaacgcgct gagctcggcg aggaatggtg
     2581 cgaccgtgcc gagcacgacc atccacacga tcagggccca caggctcacg tcgtactccg
     2641 agaggttccc gccgagtgag acctgaccac cgaggtcgac cgatcccaga gccgacagcg
     2701 gagccgcgat gttccagaag acggttgcca cgacgaaggc gccggtcagt acggcgagcg
     2761 gcggacgggt tgccaccgag tgctccccga gcaggaagta cgccgccagc gacacggccg
     2821 ctccgagtcc cgccagtacg ccgatcacgt ccagcgcgag acccgaccag atctcggtga
     2881 cgagggcgag cccaccgagc gagcacgcca gcccggccca gaggcgggcc cggacgtgct
     2941 cgccatagcc gaatcgcgcg aacagcgcca ccaggaccgg agcggtgaac tccagcagca
     3001 gggcgacacc tacgggcagc cggtcgatgg ccacgaagta cagccactgg accatcgcca
     3061 cgcccgtgat cccgaacgcc accgtggcga gcagcgcctg ccggttctcc ggaacaacga
     3121 gtctcagctc gcggcgcagg gcgacgacca gcaacagcac cagggcggtt ccggtgcagc
     3181 gcacggtcgt cagggttgcc gaacccactc cggccgactg gatgacccgg gagacgccgg
     3241 cgttgacgac gaacaacaag gcgccggtga cgacgagcag ggcgccacgg gtggggtggc
     3301 gtccgtcgcg cattggccga gcctacgcag ggcctcggcg gtgctcggac cgggttaggg
     3361 tgcggacatg acgactccgc ggttccgtcc cgccctggac acgattccgt cgtacaaggc
     3421 tggcaggccg ccggcggccg gccagcccgc gggacaaccc cgatacaagc tctcgagcaa
     3481 cgagaacccc taccccccgt tgcccggcgt cgtcgctcgg gccaccgagg ccgccgaggc
     3541 gatgaaccgc taccccgaca tgggggtcac ccggctccac gaggcgctgg cggcgaagta
     3601 ctccgtctcg acggatcaga tcgcgtccgg agccggctcg gtcgccgtcc tctaccacct
     3661 gctgcaggcc agctgcgacg acggcgacga ggtggtgtac gcctggcggt cgttcgaggc
     3721 atacccgatc gcagccaagc tgaccggggc gacaagcgtg cacgtacccg ttgacgcgga
     3781 cgcgcgtcac gacctcccgt cgatgctcga ggcgatcacc gacaagacca aggtcgtgat
     3841 cgtgtgtacg ccgaacaacc ccaccgggac gtcggtgcac caggccgagc tcgaggcgtt
     3901 ccttgccggg gttccggggc atgtcctggt ggcgatcgac gaggcctacc acgagttcgt
     3961 caccgacccg gagacgccgg acgcgctcga gctggcgcgc ggtcgcgaca acgtcgtcgt
     4021 actccggacg ttcagcaagg cctacggcct ggccggtctg cgggtcgggt acgccgtcgc
     4081 ccccgcgccg atcgccgagg ccatccgcaa gtgcgccctg ccgttcggtg tctcgtccat
     4141 cgcccaggcg gcggcggtcg cctcactcga cagcgaggcg gagctgctcg aacgcgtcga
     4201 cgagttggtg aaggagcgtt ctcgggtgca tgacgcgttg cgtcagcagg gttgggacat
     4261 ccccgacgca cagggcaact tcgtctggtt cgggctcggc gacgcgacgg tcgggttcgc
     4321 cgaggcgtgc gatgccgcgg gggtgagcgt gcgtccgttc gccggcgacg gtgcgcgggt
     4381 gacgatcggt gagcccgagg cgaacgacct gctgctcgac gtggcccagc gctggcacag
     4441 cgagcacacc ggctgacccc cggcttcccc gaattgcggg gaagtggttg tgcatgcgcc
     4501 cgaaatgcga agacgtttcc cgcaattcgc aggcccaggc aggggccggt acgacgcgga
     4561 taacgattgg cgatgcgtca gaggcatcgt ctaaagtgct acttacaacc caccggcctc
     4621 tcggatgact cggccatgcc gtcaggacgg tcctcgagcg gcgcccgact gacggcgagc
     4681 tcgccggtgt ggccgcccgt cgacctacag ggggactccg ccactgatga ccgccgccgc
     4741 cccgccagcg ctcgcacttc cgtcaccgcc ccgccagggc ggttcccgct ccttgtggcg
     4801 gctccggcgt tacatgcggc cctactacgg ctcactggcc accatgggca gcaccgccgt
     4861 cgtgggcgtc ttcgtctcga tgagcatccc gttggtcacc agggccatca tcgacggccc
     4921 gatcaccgac ggcgacatgt cggccatctg gccgctcggc ctcgccggcc tcgtcctcgg
     4981 catcaccgag gcaggtctca tcctggtccg ccggtgggtg caggcccgcg cggtgctcgg
     5041 cttcgagacc gccgtccgcg acgacctgta ccggcacatg caggcgctgc cgatggagtt
     5101 ccacggccgg tggcagagtg gtcagctgtt gtcgcgggtc acgaccgacc tcagcgcgat
     5161 ccggcggttc atgggcttcg gcctgctgtt cctcttcatc aacatcctcc agctgaccgt
     5221 cgtgacgctg ctgctcctgc acctctactg gccgttgggg ttggtggtcg cgatctcggc
     5281 agtgccgatc atcgtgctga gcaagcggtt cgagacgaag tacgtcgtcg tctcccgcgc
     5341 tgtgcaggac cagcagggcg atgtcgccac ggcggtcgag gagggggccg tcggcttccg
     5401 cgtcatcaag tcgttcgggc gccgccatca catcgagcgc cagttccgcg gccgcgcaca
     5461 gaccctctac aacacctcga tcgagaaggt ccggctgtcc tcgaagttct ggaccttcct
     5521 cgagatcatc cccaacatca gcctcgtctt cgtcctgctg ctcggcgccc tcgcggtggg
     5581 agagcacgag gtgacgctgg ggacgctggt cgccttcatc accttgatgc tgtcgctggt
     5641 ctggccgatc gaggcgctgg gcgtcatctt gtcgatggcg caggaggcga tgactgccgc
     5701 cgaccgggtc aacgagatct tcgacacgaa gtccgccatc accagcggca cccgcgagct
     5761 cgagcggccg cgcggacacc tgcgcttcga gcgcgtcggt ttccacttcc ccgactccga
     5821 tcaggacgtc ctgcacgatg tcgacctcga ggtgttgccc ggcgagacgt tggcgctggt
     5881 gggcgccacg ggtgcgggca agaccgcgct gacgagcctg gtgccgcggc tgtacgacgt
     5941 caccgcgggt cggatcacgg tcgacggggt ggacgtgcgg gaactgaccc tgccgtgcct
     6001 tcgcaccgtg gttgcgacgg cgttcgagga gccgacgctg ttctcgatga gcgcgcggga
     6061 gaacatcacg ctcgggcgtc ccgacgccga cgaggccgac atccgggacg cgatcgagac
     6121 ggcgcaggcc agcttcgtgt acgacctccc gtggggtctc gagacgcgga tcggtgagca
     6181 gggcatgtcg ctgtccggcg gtcagcggca gcggctcgcg ttggcgcgcg ccgtactggc
     6241 acgtcctcgg atcctcgtcc tcgacgacac gctttccgcc ctggacgtgc acacggaggc
     6301 gctcgtcgag caggcgcttc ggcacgtgct cgccgacgcg accggcatcg tggtggctca
     6361 tcgcgcctcg accgtgctgc tggccgacaa ggtggcactg ctgcagggcg gcacgatcac
     6421 gcatgtcggc cgccacgagg agctgctggc gaccgtcccg gcataccacg acctcctcgc
     6481 cggcgacacc gatcatttcg atgaccttcc caccgcgcag gaggacgtgg tgctgcgatg
     6541 acgacgacca acgagcgtca cgacgacgtc gacgacgagc gggccgacga cgaatcgcag
     6601 aacgtcagct ggcgcggtgt cgccgcggag gagcgcgacg agatccccgc caaggccacg
     6661 ctgctcctcc agcagcggtc gcggcgcttg ctcaagcacc tgctggcgcc gcacaagaag
     6721 ctgctcgggc tcctactggt cgcggtcctg atcgagaacg ccgcccggct ctcggtgccg
     6781 ttcctcgtca aggagggcat cgacagcggg atcccgccga tcctcgacgg tgagggaacc
     6841 agcacgctct acacgatcgt cggcgtcctg ctgcttgcgg tggtcatcca agcggtcacc
     6901 cgcgtcacct tcctcatgtt ggcgggcaag atcggccagc aggtgttgtt catcctgcga
     6961 cgccaggtgt tctcgcactt ccagaggctg tcgccggcgt tccacgagga gtacacgtcg
     7021 ggacgggtga tctcccgaca gacctcggac atcgacgcca tctacgagct gctggagacg
     7081 ggcttcgacg gtctcatcac cgccatgctg acgctgatcg gcaccgcggt gctgctcgtc
     7141 gtgctcgacg tcaagctcgg actcgtggcg ctgctgtgct tcccggcgct gctcgtcctg
     7201 acgaactggt tccggcgcga gtccgccaag gcctatcggc gtacccgcga gaccgtcgcg
     7261 gtggtcatcg tgcacttcgt cgagacgatc aacgggatgc gcgcggtgca ggcgttccgg
     7321 cgcgaggacc gcaaccagga gatcttcgag tcggtcaacg acgactaccg ccaggcgaac
     7381 gagcgcgcct tccggctggt cgggttgttc atgcccggca tcaagctgat cggcaacatc
     7441 acgatcgccg tcgtactcat ctacggcgcc cggctcgcga tcgagggcga ggtcacaccg
     7501 ggtgtgctgg cagcgttcct gctgtacctg cgccagttct tcgagccgat gcaggagata
     7561 tcgcagttct acaacacgtt ccagtcagca tcggcggcgc tcgagaagct gtccggcgta
     7621 ctcgaggagg agccgtcggt gccggagccg aagcatccgg tgccgctcgt cgaggcgcac
     7681 ggcgcactgc acctggacgg ggtcgggttc gagtacgtcg ccgggcgacc ggtgctccct
     7741 gacctcgagc tggaggttcc ggccggtatg acgttggccc tggtcgggac cactggtgcc
     7801 ggcaagacga ccctggccaa gctcgtgtcg cgcttctacg acccgacgcg cggtcgcatc
     7861 acgctggacg ggatcgacct gcgtgacctc gcagaggacg acctgcgcaa ggcggtcgtg
     7921 atggtgacgc aggagaactt cctgttcaac gggacggtgg ccgacaacat ccggttcggc
     7981 aagccggacg cgacgatgga ggaggtggtg gcggccgcgc aggcgatcgg cgcccacgac
     8041 ttcatcatcg gcatgccgga gggctacgaa accgatgtcg ccaaccgtgg cggtcggctg
     8101 tcggccggtg agcgacagct ggtggcgttc gcccgggcgt tcctggccaa cccggccgtg
     8161 ctcatcctgg acgaggcgac ctcgagcctg gacgtgccgt ccgaacggct ggtgcagcac
     8221 gcgctgcgga ccatcctggc cgaccggacc gcgatcatca tcgcgcaccg gctctcgacg
     8281 gtggagatcg ccgaccgggt gctggtgatg gagcacgggc ggatcgtcga ggacggcacg
     8341 cccgacgagc tcatcgccgg tggcgaggga cggttcgccg acctccacaa cgcctggctc
     8401 gcctccctcg cctgaccgtc cacgccgagg tggcgatgga cgttcgggaa tcggcgtgtc
     8461 tccaacgaaa cctgccacct cggcaggagg ggtggacgtc agaggtcggg gagtgcgggg
     8521 gcaaccttgg gcggcgtacg ccggagctgt tgttcgacga gcgccagcgc gcggtcgagc
     8581 tggacgtcgt tgcccgcgac ccggtcctga ggcgcgacct cgacctcgac gtccgggtcc
     8641 accccgtagt tctcgacccc ccagccgaag tcctcgaacc agaaggcgta ccgcggctgc
     8701 gtcaccagtg ttccgtcgat gagccggtag cggccgtcga tcccgacgac gccgccccag
     8761 gtacgcgtcc ccacgacctg gccgagcccg agtgactgga tggcggcggt cacgatgtcg
     8821 ccgtcggagc cggcgtacat gtcggtcacg gccacgagag ggccgcgccg tgcgtcgagt
     8881 gggtacgact ccggctcgta cccgcgggtg aggtcccagc cgatcacccg gcgggccagc
     8941 ttctcgacca cgagctggga cgtgtgtccg ccgcggttgg cgcggacgtc gacgatgagg
     9001 gcgtcgcggc gcatctcggt gcgcaggtca cggtggagct gcgcccaacc gccggagacc
     9061 atgtcgggga cgtgcaggta tccgacccgg ccggctgtct gctcgtgcac gtaccgtcgg
     9121 cggtcgttga cccagtcctg ataccgcagc ggcatctcgt cagccagtgg cacgacgaca
     9181 gcccgccggg cagcaccgcc gtccgccggc gcgatcgtca gctcgaccgg cttgtccgcc
     9241 gtgccgagca tcaacgcgta cggactcctg gtacgactcg gccgacgccc gtcgaccgcg
     9301 acgatggcgt cgccgacacg cgcgccgacg cccggcgccg tgagcggtga ccgtgcgttc
     9361 cggtcggaag gctcgcctgg aatcacgcgg gcgatccgcc aggcatcgtc ggcgtactcg
     9421 aggtcggcac cgagcaggcc ctgcctgcgg gtcggttcga cgtcgtccgg gtccggcaag
     9481 acgtaggcgt gcgaggtccc gagctcaccc tggagctccc agatgaggtc gacgaggtcg
     9541 tcgtgggacc ccaaccgcga caacagcggc gcgtatcgtt cgcgtgcggc cccccagtcg
     9601 gtgccgccca tgtcggggcg ccagtagtgg tcacgcatca gccgccacgc ctcgtcgtac
     9661 atctggcgcc actcggcctg cggctcgatc tcgatgcgga cccggttgag gtcgaccttg
     9721 ttctcgtcct gtgcggcggc ctggtcgtct ggccgcttgt ccaccttcgt ggtcgacgag
     9781 acg
//
LOCUS       DATBFZ010000071         5395 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_5931271, whole genome shotgun sequence.
ACCESSION   DATBFZ010000071 DATBFZ010000000
VERSION     DATBFZ010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 5395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5395
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5931271"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1023
                     /locus_tag="VH419_02135"
     CDS             <1..1023
                     /locus_tag="VH419_02135"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEY7042446.1"
                     /translation="LWLEADRMAFLDDVLTQENFANQVAVVAQERSQRMDNVPYGTVF
                     ERALPLIFDADHPYGHLPIGNMQHLAEADLDEVSAFHTTYYMPNNAVLSIVGDIETEE
                     GFAKAARYFDHIEPGDVPKREIPPALPPLSEPRRLDHEEDVPSPGLWLAYRLPVDAPD
                     SRELAAVSLASSIVGENETSRLYRRLVRQDRLALSAGLGLNTLIAGNSLGLGSMRALP
                     GQDLEELASVVSDVLHRFAGDGPSDRELQMARAQAERDWLDEMGTAAGRADAISACTL
                     LFDDAEALNQRLPLLRSITGEEVREAAETWLLPGLNAQVRVHPEGPAERAALAGEMPL
                     EELEEQ"
     gene            1026..2399
                     /locus_tag="VH419_02140"
     CDS             1026..2399
                     /locus_tag="VH419_02140"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEY7042447.1"
                     /translation="MIATPPPVSEVSNWRFPHHIEHRLDNGLRVLRYDCPGQYVVAAS
                     MLFDLPLTAEPREVEGVAGLVGRCLTRGAAGRSAEEFADALALCGADLDASAFPDGFV
                     VRLSAPTVELTDAFGLMADALMAPEFDVDEFAHEQRLRLQEIDQAAAYPQHVAVEHLN
                     AALFGEHRAARPVGGSATSVRAVGRDDVVEYAARNLDPRGATLVIAGDFADLDLESLL
                     RDTFGGWQGADIAQPVHPVVPAASSDRPQVLLVDWPDAPQSTLRLAGPGITRGDSRWP
                     GMFVANFAIGGNFSSRINTVLREEKGFTYGAGSSLDTGRSTGLVAISTAVRTDATAEA
                     LTEVIAIMRAAPGSITDEEVAMASRAATESAALGFERADSVVGRVELLLSQGLPLDHV
                     DVNLERIRTVTTAAANEALTSILRPDAFTVVVVGAADELRRPLADWGYAGVTEVAPTR
                     TSTATPD"
     gene            complement(2396..4027)
                     /gene="pgm"
                     /locus_tag="VH419_02145"
     CDS             complement(2396..4027)
                     /gene="pgm"
                     /locus_tag="VH419_02145"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002548824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HEY7042448.1"
                     /translation="MAHERAGQPAQASDLIDVDTVVAAYYDRKPDPDDPGQRVAFGTS
                     GHRGSSLDTAFNEPHIVATTQAIVEYRAGQGVDGPLLIGRDPHALSEPAWQTALEVLV
                     AAGVTVLVDDRDGYTPTPAVSHAILRLNREGRHGRVDGIVVTPSHNPPRDGGFKYNPP
                     HGGPADSDATNWIQDRANVLLADGVDDVRRVDADQARKTAGRFDFLASYVDDLPDVLD
                     LDAIKNAGVRIGADPMGGASVAYWGEIAERHGLDLTVVNPKVDPTWRFMTLDTDGKIR
                     MDCSSPNAMASLIAQRASYDIATGNDADADRHGIVTPDAGLLNPNHFLAVAIGHVYGH
                     RDAWPSECAVGKTLVSSSMIDRVAGELGRRLLEVPVGFKWFVPGLLDGSVGFGGEESA
                     GASFLRRDGTTWTTDKDGILLALLASEILAVTGKTPSQLYAELVQRHGDPAYARVDAP
                     ASREQKARLGRLDTGDITATEVAGEPITAMFTKAPGNDAAIGGIKVCTESAWFAARPS
                     GTEDVYKIYAESFRGPDHLALVQQEARNVVNAALG"
     gene            4175..5077
                     /locus_tag="VH419_02150"
     CDS             4175..5077
                     /locus_tag="VH419_02150"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY7042449.1"
                     /translation="MKVFVTGATGVLGRAAVAALVHAGHDVTGVARTDEKAAVLTGLG
                     ATARNVSLFDHDGLVSAFGGFDAVCNLATHFPVGMAGLRPGAWKVNDRLRVEGSKTVA
                     QAARGAGVRRLVQESVSLLYADGGDDWITEESTLSVSRSLDPAAMAETNAGMFACRSR
                     RPVVLRFGSIIGNDPITKWRLAQARAGRPIGLGDPNGWAHLVHPEDAGTAVSAALDAP
                     DGVYNVGAEPVRRQEMVAAFAHAVGRDELGFMSSMLVRLGGDRLSPLTRSHRVSSALL
                     HESTGWKPLHDQFTTSWLTDLTGI"
BASE COUNT          798 a         1851 c         1903 g          843 t
ORIGIN      
        1 ctgtggctcg aggccgaccg gatggcgttc ctcgacgacg tactgaccca ggagaacttc
       61 gccaaccagg ttgcggtggt ggctcaggag cgcagccagc ggatggacaa cgtcccgtac
      121 ggcaccgtgt tcgagagggc gctgccgctg atcttcgacg ccgaccaccc gtacgggcat
      181 ctgcccatcg gcaacatgca gcacctcgcc gaggcggacc tcgacgaagt gagcgcgttt
      241 cacacgacct actacatgcc gaacaacgcc gtgctctcga tcgtcggtga catcgagacg
      301 gaagagggtt tcgcgaaggc cgccaggtac ttcgaccaca tcgagcccgg cgacgtgccc
      361 aagcgggaga ttccaccggc gctgccaccg ctgtcggagc cgcgtcgtct cgaccacgag
      421 gaggacgtgc cgtcaccagg cctgtggctg gcctaccggc tcccggtgga cgcgccggac
      481 tcgcgtgagc tcgcggcggt gagcttggcc agcagcatcg tcggtgagaa cgagaccagc
      541 cggctgtacc ggcgactcgt ccgccaggac cgcctcgcgt tgtccgccgg gctcggcctg
      601 aacaccctca tcgccggaaa ctcgcttggt ctcggctcca tgcgcgcgct gcccggccag
      661 gacctggagg agctcgcgtc ggtagtgagc gacgtgcttc atcgtttcgc cggcgacggc
      721 ccgagcgatc gagagctcca gatggcgcgc gcacaggcag agcgtgactg gctcgacgag
      781 atgggaactg ctgccgggcg cgccgacgcg atctctgcgt gcacgctgct gttcgacgac
      841 gcggaggcgc tgaaccagcg gttgccactg ctgcgcagta tcaccggcga ggaggtgcgc
      901 gaagccgccg agacgtggct gcttccgggg ttgaacgcgc aggtgcgggt ccatcccgag
      961 gggcctgccg agcgcgcggc gctggcaggg gagatgccgc tggaggagct ggaggagcaa
     1021 tgacggtgat cgcgaccccg cctccggtga gcgaggtctc caactggcgg ttcccgcacc
     1081 acatcgagca ccggctcgac aacgggctgc gcgtcctccg gtacgactgc cccggccagt
     1141 acgtcgtggc ggcgtcgatg ctgttcgacc tgccactcac ggccgagccg cgcgaggtcg
     1201 aaggggtagc ggggctggtc ggacgatgcc tcacccgtgg cgctgccggc cggtccgcgg
     1261 aggagttcgc cgatgcgctc gcgctgtgcg gagcggacct ggacgcttcg gcgttcccgg
     1321 acggcttcgt cgtccggctt tccgcgccga ccgtcgagct gaccgacgca ttcgggctca
     1381 tggccgatgc actgatggcg ccggagttcg acgtcgacga gttcgcccac gagcaacgac
     1441 tccggctcca ggagatcgac caggcggcgg cgtacccgca acacgtcgcc gtcgagcacc
     1501 tcaacgcagc actgttcggc gagcaccgcg ccgctcgacc cgtcggtggt agcgcgacgt
     1561 ctgtccgagc agtcgggcga gacgacgtcg tggagtacgc cgcccggaat ctcgacccac
     1621 gcggggcgac gctcgtcatc gcgggtgact tcgccgacct cgacctggag tcgctgctgc
     1681 gggacacctt cggaggatgg cagggcgcgg acatcgcgca gcccgtgcat cccgtcgtgc
     1741 ctgctgcctc ctcggaccgg ccgcaggtgc tgctcgtcga ctggccggat gcaccgcagt
     1801 cgaccctccg gctcgccggg ccggggatca cccgaggcga cagccggtgg ccgggcatgt
     1861 tcgtcgccaa cttcgccatc ggcgggaact tctcctcccg catcaacacc gtgctgcgcg
     1921 aggagaaggg tttcacctac ggtgccgggt ccagcctcga caccgggcgg tccaccggtc
     1981 tggtggcgat ctccaccgcg gtccggacgg acgcgacggc cgaggcgttg accgaggtca
     2041 tcgcgatcat gcgcgccgcg cccggctcga tcaccgacga ggaggtcgcg atggcgtcac
     2101 gtgccgcgac cgagtcggcc gcgctggggt tcgagcgagc cgacagcgtc gtcggccggg
     2161 tcgagctgct gctgtcgcaa gggcttccgc tggatcacgt cgacgtgaac ctggagcgga
     2221 ttcgcacggt cacgacggcg gccgccaacg aggcgctgac ctcgatcctg cggccggacg
     2281 cgttcaccgt cgtcgtcgtc ggagctgcag acgagctgcg tcgcccgctc gccgactggg
     2341 gctacgccgg cgtgacggag gtcgccccca cccgcacgtc gacggcaacg ccggactaac
     2401 cgagtgcggc gttgacgacg ttgcgggcct cctgctgcac gagcgcgagg tggtcgggtc
     2461 cacggaagga ctcggcgtag atcttgtaga cgtcctcggt gccggacgga cgggctgcga
     2521 accaggcaga ctcggtgcac accttgatgc cgccgatcgc agcgtcgttg ccgggcgcct
     2581 tcgtgaacat cgccgtgatc ggttcgccgg ccacctctgt ggcggtgatg tcgccggtgt
     2641 ccagcctgcc gagccgggcc ttctgttcgc gggacgcggg cgcgtccaca cgggcgtacg
     2701 cagggtcgcc gtgccgctgg accagctcgg cgtacagctg gctcggcgtc ttgccggtga
     2761 cggcgagaat ctcgctcgcc agcaacgcca gcaggatgcc gtccttgtcg gtcgtccagg
     2821 tggtcccgtc gcgacgcagg aacgacgcgc cggccgactc ctcgccgccg aagccgaccg
     2881 agccgtcgag gagcccgggg acgaaccact tgaatcccac cggcacctcc agcagccgac
     2941 gccccagctc gccggcgacc cggtcgatca tcgacgagct caccagcgtt ttgccgacgg
     3001 cacactccga tggccaggcg tcccggtgcc cgtacacgtg tccgatcgcc acggcaagga
     3061 agtggttggg gttcagcagt ccggcgtccg gggtcacgat gccgtgccgg tcggcgtcgg
     3121 catcgttgcc ggtcgcgatg tcgtacgacg cacgttgtgc gatcagcgac gccatcgcgt
     3181 tgggggacga gcagtccatc cggatcttgc cgtcggtgtc cagcgtcatg aaccgccagg
     3241 tcgggtcgac cttcgggttc accacagtga ggtcgagacc gtgtcgttcg gcgatctctc
     3301 cccagtacgc cacgctggcg ccacccatgg ggtcagcgcc gatccgtacg ccggcgttct
     3361 tgatcgcatc gaggtcaagc acgtcgggaa ggtcgtcgac gtaggacgcg aggaagtcga
     3421 accgaccggc ggtcttgcgg gcctggtcgg cgtccacgcg gcgtacgtcg tcgacgccgt
     3481 cggcgagcaa cacgttggcc cgatcctgga tccagttcgt ggcgtcggag tcggccggtc
     3541 cgccgtgcgg cgggttgtac ttgaagccac cgtcgcgggg cgggttgtgc gacggtgtca
     3601 cgacgatccc gtcgacccga ccgtggcggc cctcccggtt gagccgaagg atcgcgtgcg
     3661 agaccgcggg tgtcggcgtg tagccgtccc gatcgtcgac gagcacggtg acaccggcgg
     3721 ccacgagcac ctccagcgcc gtctgccagg cgggttcgga gagcgcgtgc gggtcgcggc
     3781 cgatgagcag cggaccgtcg accccttgac cggcgcggta ctcgacgatc gcctgggtcg
     3841 tcgcgacgat gtgcggctcg ttgaacgccg tgtcgaggct cgagccacga tgcccggagg
     3901 tcccgaacgc cacccgctga ccgggatcgt ccgggtccgg cttgcggtcg tagtacgcgg
     3961 ccaccaccgt gtcgacgtcg atcaggtcgg acgcttgggc cggttggccg gcacgctcat
     4021 gagccatgcg gcgatcctcg cccaccccga cagcgctgtc gaccgatgga ctctggcggg
     4081 tacttcggag ttcgtctcac gacgtgggac gtgctctgtt atcttctcgt tatctgtggg
     4141 cacctagcgc ctgcagaccc ctgtgaggtg ccgagtgaag gtcttcgtga cgggagccac
     4201 cggcgtgctc gggcgcgccg cggttgccgc actcgtccat gccggccatg acgtcaccgg
     4261 cgttgcccgc accgacgaga aggcggccgt tctcaccggc ctcggcgcta ccgcgcgcaa
     4321 cgtctcgttg ttcgaccacg acggtctcgt ctcagcgttc ggcgggttcg acgcggtgtg
     4381 caacctcgcc acgcacttcc cggtcggcat ggcagggctg cgtcccggcg cgtggaaggt
     4441 caacgaccgg cttcgtgtcg agggctccaa gacggtcgcg caggcggctc ggggcgccgg
     4501 cgtacgacgg ctcgtgcagg agagcgtgtc cttgttgtac gccgacggag gcgatgactg
     4561 gatcaccgag gagagcacgc tgtcggtgtc gcgctcgctc gacccggcgg cgatggccga
     4621 gacgaacgcc ggcatgttcg cctgccgcag tcggcgtccg gtcgtgctgc ggttcggcag
     4681 catcatcggc aacgatccga tcaccaagtg gcgactcgcg caggcacgcg cgggacggcc
     4741 gatcgggctc ggcgatccca acgggtgggc ccatctcgtc catcccgagg acgccggcac
     4801 ggccgtctcg gcggcgctgg acgcacccga cggcgtctac aacgtcggag ccgagccggt
     4861 gcggaggcag gagatggtcg cagccttcgc ccatgccgtc ggacgtgatg agctcggctt
     4921 catgtcgagc atgctcgtcc ggctgggtgg cgaccggttg tctccgctga cgcgctccca
     4981 ccgcgtctcg tccgccctgc tgcacgagtc cacgggatgg aaaccgttgc atgaccagtt
     5041 cacgacttca tggttgaccg acctgacggg gatctgagcc ccacgaacga cgccgctgac
     5101 gacgaggacg ggcgcgaccc ggccgaaccg gcccgtacgc cggcggagcc aaaagatcga
     5161 cggcctcgcc accggcccac ggtcgccgag gtcttcggcg atgtgctccc ggacaccacc
     5221 ggagacgaac gcgacgaaga cagcgaccgg gctgcaggaa ggtcatcgcg ggacgaggag
     5281 atcctgcgcg acgtcccgcc gcatcactga gcgcccttcg gcggtcgagc ccgtcgagac
     5341 ccccttgatc tcgacgggct cgatcagcgg gcttcggcgg tcagcgggct tcggc
//
LOCUS       DATBFZ010000072        19494 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_47938, whole genome shotgun sequence.
ACCESSION   DATBFZ010000072 DATBFZ010000000
VERSION     DATBFZ010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 19494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19494
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47938"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2301)
                     /locus_tag="VH419_02155"
     CDS             complement(<1..2301)
                     /locus_tag="VH419_02155"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEY7042450.1"
                     /translation="MNLSALVQVLHAEGPMSRSELVERTGLTRSAIRRLVGELVAAGL
                     VIEQRGESQGSPGRPSPVVRLDPTGAVVLALEIAVDSLAMAVVGVGGQVLELARVDRS
                     RGHLAPDDLVADLVALADGASARWRNDALVGIGVAVVGVVRRDDGFVSIAPNLGWRDV
                     ALGVELSAALGAPAPVHVANEADLGALAEHRRGAAVGVDDVIFLSGEVGVGGGLIVGG
                     RPLTGVAGFGGEVGHVPINPVGGAACRCGAVGCWETEIGEEALLRRAGRPADGGSAAV
                     EAVLDDAGRGDTTALGAIAEIGDWLGLGLSGLVNTLNPARVVLGGRFARLHPFVAPRV
                     EAALDRRALAAPRSLVDVVPATLGVDAPLLGAAELAFGPILDDPAAWFHRSRKEAEVA
                     SSRSPHGRVVASQARSHRPKGGLANMHKFSRAHVGLRHTGEETRMHRRRSVVLAMSAA
                     VVLAACGSSSGGEASNTTAAAGGAATTAAAAATTAAAAPATTAAAGGAATTAAAGGGG
                     AAGGCKVGVSWNNYQEERWAKWDEPAIKAAIAAGGGTYISNDAKSSAETQANNVDNLI
                     SQGAKVLIILAQDGTAIKPSVASALQQGVPVIAYDRLIEDPKALYITFDNVEVGRLQA
                     ESIFGAVPKGNYIIIKGNKADANADFLRSGMDKVIKPAVDSGDIKIVGETYTDNWDPS
                     KAQTETEQFLTSANNDVQAVLSENDGMAGGVVAALQAQGLAGKVPVSGQDGDQPALNR
                     IALGTQTVDVWKDARMLGKAAGDAAIQ"
     gene            2519..4108
                     /locus_tag="VH419_02160"
     CDS             2519..4108
                     /locus_tag="VH419_02160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042451.1"
                     /translation="MTSRTLTLGRTTYPVLLPNLRDPRLHVAAVIITIHVLGQLGLHF
                     HVSVPQILSAILTCALLEVALTFRAQRAFVWPASAMLTGSGVALILRVPDTPLNDPWT
                     THAWYVFAGVAAFSLLTKYVIRYHGSHVFNPSNIGLVVAFLVLGSERVEPLDFWWAPL
                     NLWMITAYAVILAGGLLVTARLRMLAVAVTFWLSLAAGVAVLAASGHCMTARWAFAPV
                     CGFDFWRVIVISPEVLIFLFFMITDPKTVPSGRVGRVVFGLLVAVVSTLLMAPQTNEF
                     GTKVALLGGLVLVCAARPVLDRLVPQPRTAADRLGRFASRMATGGGSGGWLRPVLRIA
                     MIAAAVAVVGAGIVTAGTPARGVVVPGVDDVVGRVPHDVDPATFPKISVEQDVIDWNH
                     EIAGPGVREIALTLVENLQLENQALLRADPKILEAVDHGDRLDEMRDRLAQAKSTGTT
                     VVERYQIKDVDVALLVPFGKQDGLSLGFRSRGLETKETYDSDGHLRASTSTPFAKTFV
                     VRRATGARWLNVAVLPPAAGT"
     gene            4166..5857
                     /locus_tag="VH419_02165"
     CDS             4166..5857
                     /locus_tag="VH419_02165"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HEY7042452.1"
                     /translation="MPEEPAVVHPDPASHRTRRSRHWPILVGLVAMLVVVGGVVVAVR
                     SRSSSSYVPSALHYVEEAAAAGIDHQYAGGSQFFVGGGVAAFDCNEDGRPDLFFAGGS
                     GPAALYRNESPTGGALRFARVASSVTDLTSVTGAYPLDIDGDGHLDLAVLRVGGNVVL
                     RGRGNCQFERANERFGIDGGNAWTVAFSATWEGSNALPTLAFGNYRTPDGESCADSEL
                     VRPAASGDTYAAAIPLHPGYCTLSVLFSDWSRTGKRDLRMTNDRNYYREGEEQLWRLE
                     PGQAPRLYTAGEGWRPMQIWGMGIASQDVTGDGYPEVFLTSQGDNKLQTLAAGATRPS
                     YADIALDRGVTAQRPFTGGDVLPSTAWHPEFEDVNNDGVLDLFVSKGNVEAQPDYARR
                     DPSNLFLGEADGTFVEGAEAAGIVRYERARGASLVDLNLDGMLDLVVVDRAANVQVWR
                     NVGRGDAAHPAPMGHWIAVGLQQPAPDVDAIGAWLDVKVDGRTVTREVTVGGGHAGGK
                     LGWFHFGLGDAKEAQVRVTWPDGQKGSWMTMQADQMATIERGSPAPARWLPPPAP"
     gene            5854..7248
                     /locus_tag="VH419_02170"
     CDS             5854..7248
                     /locus_tag="VH419_02170"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEY7042453.1"
                     /translation="MNATRTTGAARLAEVELPDFGMPRAEPLLPASLYAARLDRLRAR
                     MEARGHEHIVVWADREHSANLAYLTGFDPRFEEAALIVGSTSDPALLVGNECFGMAEA
                     APLRTRTVRFQDFSLPNQPRDASCALADILGGEGIAAGRRVGVVGWKTYASRDTIELP
                     AFVVDALRQATGSHALVENATDLFIDAEDGLRVVNEVEQLAAFEWAACQTSRGVRNVV
                     TGVQPGMTEREAARLLEWNGAPLSCHLMLTAGPRAKLGLLSPGDRPIERGDPLTTAFG
                     IWGGLNCRAGFVVEDAAELPDGIRDYVDRLVAPYFEAVAEWYEALHVGQTGGELQAIV
                     DRHLGDAFFGVFLNPGHQLHLDEWVNSPVFPGSTIELRSGMALQCDIIPTTGTPYFTT
                     NIEDGLALADDSLRAAFRSLYPDAWGRVQARRRFMSEALGIELHPDVMPFSNLAAHLP
                     PFLMRPDRAMTLVR"
     gene            7302..8897
                     /locus_tag="VH419_02175"
     CDS             7302..8897
                     /locus_tag="VH419_02175"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HEY7042454.1"
                     /translation="MTDQPKTEQPNLHDWAPLVDDLAARKSRAVEMGGPKQVDRQRSM
                     GKLPVRDRLDLLLDPGSFVELGMLADSMDATLTAERGYLAADGMVAGIGAIDGRRVAI
                     CAYDFTVLAGSMGNIGEQKTARVRELALRQRIPIVWLLDSAGARIQSSSGSTFAAAGA
                     LFREQVTMSGVVPQVAAMLGHCAAGTAYIPALADFIPMVKGTSSMALGGRHLVRAATG
                     EDVTEEEMGGSEVHTKVSGVADLEVASDEECLDVVRRYLSFFPSHNGEAPPVRPTNDP
                     VDRRVEELYRLVPTAPRRAYDMNKVVPLLCDDGEFFPIKPAFARNLVTGLGRIGGLPV
                     GFLASNPMVLGAALDVNAADKAARFVWLCDAFGIPLVFLHDVPGFIVGSAVEKQGIIR
                     HGAKMLFAVSEATVPKVSIVFRKSYGAGYFVMNGTAYEADYIAIWPTAEIAVMGPDGM
                     VEITQRRVLDQYTDPKERADAKIRLAEEFRKNIDPYIAAGHAAVDDVIDPADTRFAIW
                     KGLQVSKTKHVERPWRKHGVPPV"
     gene            9195..10376
                     /locus_tag="VH419_02180"
     CDS             9195..10376
                     /locus_tag="VH419_02180"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009071871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEY7042455.1"
                     /translation="MGEQYLTSKLQGFGTTIFAEMSALAVSTGSINLGQGFPDEDGPA
                     EVLDAALYAISHGNGNQYPPGAGVPELRHAVVGHQHRFYGFEFDPDTEVLVTCGATEA
                     IAAAMLALLDTGDEAIAFEPYYDSYAACISMAGAVRRPVTLRPPDYALDVDALRAAIT
                     PRTKLILLNTPHNPTGKVFTADELAAIAAVAQEHDLVVVADEVYEHLTFDDVPHVPIA
                     TVPGMWERTVTIGSAGKSFSFTGWKVGWATGPAPLITAVRTAKQFLTYVASGPFQYAA
                     AIGLGLPDTYFESVRATLQEKRDRLADGLSDAGFTVFPPQGTYFITADIRALGEDDGM
                     AFCRSLPDRCGVVAIPTIVFYDDVDAGRPLVRFAFCKRLPVLDEAVERLRSLRTGRSG
                     T"
     gene            10373..11146
                     /locus_tag="VH419_02185"
     CDS             10373..11146
                     /locus_tag="VH419_02185"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEY7042456.1"
                     /translation="MRIAAIQHDIVWEDGDATRRHVTPMVAAAAASGARLAVLTEMFA
                     TGFSLEAERIAEAEDGPTSTWLRGQAAEHGLWLYGSVPERRPDGGRPRNVGILAGPDG
                     SVHRYAKIHPFSYAREHESYDAGDRTVTVEVEGVRCSLFVCYDLRFADDWWPLAPSTD
                     VYLCCANWPETRRAHWIALLQARAIENQAYVVGVNRVGEGGGLSYSGDSRIFDPLGEM
                     LAGAARTETILLADVDPAEVVKVRDRFRFLQDRRTGAPT"
     gene            11143..11358
                     /locus_tag="VH419_02190"
     CDS             11143..11358
                     /locus_tag="VH419_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011753681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding carrier protein"
                     /protein_id="HEY7042457.1"
                     /translation="MTEVRAEITANVWQVRTEVGADVAEGDELVILESMKMEIPVAAP
                     GPGRVAELRVVPDDKVQEGDVLVVLDP"
     gene            11546..16312
                     /locus_tag="VH419_02195"
     CDS             11546..16312
                     /locus_tag="VH419_02195"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEY7042458.1"
                     /translation="MRVRRLAVGGCLAVAAMTMLDLATAPPPAVAAVTPAFVQVRANE
                     VSSGTTNSLAFTNAPKAGNLIVVYVLWNNAGAVTLSDSRGDTYASAGSRTTWGSNYSA
                     QTFYAKNIVGGATTVTATFGTSLTSFGIVYVHEYSGVDKTNPLDVTATATGTAKAMSS
                     GNVTTTNATDLLFGAGASSNAVNQGGTGYTTRSTASGNRTEDRVVTSTGSYAATASQN
                     GNAWVMQIVAFKADAGGTGDTTPPSVPTGLTATAVSQTQINLAWNASSDNVGVTGYNV
                     FRNGTLVTTVGTNSYQNSGLSPGTTYTYTVSAFDAAGNTSAPSTSASATTQSPPPDIT
                     PPTVSLTAPAAGATVSATLSVTANAADNVGVVGVQFLLDGTNLGGEDTAAPYSTSWDT
                     TTATNGSHTLSAKARDAAGNTTTSATVSVTVSNTAPPPGGPQAAYAFDEGAGTSAADA
                     SGHGITGTLTNDASWTAGKYGTALDLDGAGDFVDLGNPTALRMTGSMTLSGWIYATAF
                     PGDDAPIVSKRGSNGFQLDVTPDTGPRTIGFKLTNSSGGDMIRYGATALQVNSWYHVV
                     GVYNASAVTLHVYLNGQLDDGALVGTVTNSQQNSSQDVDVGQRPSGGAFFDGRIDDVR
                     IYNKAQSAAEIQADMLTPLGSGSSPDPTPPSVVIDAPVNGAQVSDIITVTADAEDNVG
                     VTGVQFFVDGQPAGAEDTVAPYGLQWDTRTVSNGAHTLTAQARDAAGNTAISAGVTVN
                     VANANFFQNEVLATGFDLPTAMKFMPDGRLLVVELAGTVLIVPPPYTQPDPTPFLHLT
                     NVGSAGVQQGLYDIALDPNFATNHFFYVFYTLGSPNRDRLSRFTANATNTGTVAGSEL
                     VLYQDPEDANAEHHGGAIMFGNDGKLYVTTGEHFNAGESQDLTKPRGKILRLNPDGTV
                     PTDDPFYDGSGPHWDSIWALGLRNPFRAYYDSPTGRMFVGDVGGNDASTAKEEINIGA
                     RGANYGWPDSEGPCSGQCTSPLYYYPHNGRDSAVVAGFVYHGSQFPSSYQGSFFFADY
                     TQNWIKRLTLNPDGTLANVFNFEPADGSVDGPYGDIVYLTQGPEGALYYLDLGYSDIG
                     GTFGLSKLRRIRYIQSDQPPIVNASANPTSGAAPLTVNFSSAGSSDPEGHALTYSWDF
                     GDGTTSTAANPSHTYTQAGPYAARLTVSDGTNNSISPPIAINVGNKPTATITSPQDGV
                     FFKAGDVISFSGDATDAEDGTLPASAFTWNIDFLHDGHVHPGVPQTGIKSGTFTIPTT
                     GHDFSGTTRYRITLTVTDSSGLTDSKFVTVWPTKVNLSFDTVPSGLTLNLDGIAKTTP
                     FVYDTLVGFNHTIQAPDQSVGSNNYTFASWSDGGAAQHVIVVPATDQAYRATYNVTSP
                     PPTPAFVQITSTTPQSSLATVTLAYPGGQVGGDTNVIAVGWNDVSSTITSVTDSAGNT
                     YQVATPMARGPAVSQAIYYAKNIKAAAAGTNTVTVRFSAAAPFPDIRAAEYSGLDLLN
                     PFDKTGSASGTSATATSGSVTTASATELLFGAGMTTGVFNGSASGYTTRVITPQDGDI
                     ANDRNVTAVGSYTAGATLSGSAGWLMQIASFRAAGQ"
     gene            complement(16343..17089)
                     /locus_tag="VH419_02200"
     CDS             complement(16343..17089)
                     /locus_tag="VH419_02200"
                     /inference="COORDINATES: protein motif:HMM:NF016119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class E sortase"
                     /protein_id="HEY7042459.1"
                     /translation="MDDLKKGPGHYPDTPLPGHIGNVGIAGHRTTFGHPFFRINELEP
                     GDDVILTTATGRYVYKVTEQFIVSPSQSEVLAPTPDAELTLTSCHPRYSARKRIIIKA
                     KLDAAASSPLAPTAPPPTVAPTTVAPSTIAPTTAAPQSAASTTTSAASAPPATTAAAL
                     APTPPSVADQPIAGDTPDALEKGWFADSSAWGPVVCWGLLVVACSLLGWGLRRWTGRR
                     WVGVIAAVAPFVVCLYFFFENVNRLLPPNI"
     gene            17100..17390
                     /locus_tag="VH419_02205"
     CDS             17100..17390
                     /locus_tag="VH419_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042460.1"
                     /translation="MTTIQFSPIFGASRRAIGSPGAGAAVVGGGAGAVVATVSGSVDE
                     ELDGTDDATDDEDDDDVDVGATVVVVVASTSSNWLESWFCDWRVWIPVPHSW"
     gene            complement(17378..17542)
                     /locus_tag="VH419_02210"
     CDS             complement(17378..17542)
                     /locus_tag="VH419_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042461.1"
                     /translation="MNGEATSADWDPPLPSEKAPPPHPRKDWRWWVGLVGRILVVTGL
                     LLLAFVAYQL"
     gene            17639..18397
                     /gene="ccmA"
                     /locus_tag="VH419_02215"
     CDS             17639..18397
                     /gene="ccmA"
                     /locus_tag="VH419_02215"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HEY7042462.1"
                     /translation="MEPVIHLRDAVAVLGRFPALAGASLTVERGEIVLLKGPNGAGKT
                     TLLRLCAGLLPLERGEARILGVDLATDRRRVRARVGLLGHSNGLYDDLSVAENVRFWA
                     QTVGATSAEVDAALARLGLAGRLAETKVARLSAGQRRRTALAALVARRPELWLLDEPH
                     AGLDAEGRDVIDGLLHAAVAAGATVMVASHERERAGALAHRRVLVVGGQILDDGPHAA
                     GGRMARAVDEQRVASSPAGAASPTEPAPAGATPC"
     gene            18391..19074
                     /locus_tag="VH419_02220"
     CDS             18391..19074
                     /locus_tag="VH419_02220"
                     /inference="COORDINATES: protein motif:HMM:NF015344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HEY7042463.1"
                     /translation="MLRTLRLVAGKDLRIEARSRVAANQVLPLAVLVLVLFAFALDPD
                     SGVLRRATPGLFWLTVLFALVFTVQRSFALETADDAQDMLRLSALEPAGIFLGKAAAL
                     LAELLLLELGLGVGIIVLYDAPTRAAGVVLLLVAAVAATCGLAAVGTLYGGLAAGLRV
                     RDTLLPLLLLPVVAPVLIGASRAFEAALGIGDRAISEGWPWVGLLTVFALAYTALGLV
                     VFGPLMEES"
     gene            19071..>19494
                     /gene="ccsA"
                     /locus_tag="VH419_02225"
     CDS             19071..>19494
                     /gene="ccsA"
                     /locus_tag="VH419_02225"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEY7042464.1"
                     /translation="MTTGGATAAETGTVSVAPTSDAPHADSPARRTSATGTKATRVIG
                     GLALAGIPLLLLLGLVTSPEDVEMGDSVRIMYVHVPSAWLAYLAFGVTAVGSAGYLWR
                     RTRSLAWDRLAGASAEIGVLFTGLALATGMLWGRPTWGV"
BASE COUNT         3008 a         6844 c         6687 g         2955 t
ORIGIN      
        1 ctggatggcg gcatcaccgg ccgccttgcc cagcatgcgg gcgtccttcc acacgtcgac
       61 ggtctgcgtg ccgagagcga tccggttgag ggccggctga tcgccgtcct gacccgacac
      121 cggcaccttg cccgcaagcc cctgcgcctg cagggcggcc accacgccgc cggccatgcc
      181 gtcgttctcg gagagcacgg cttgcacgtc gttgttggcc gacgtcagaa actgctcggt
      241 ctccgtctgg gccttcgacg ggtcccagtt gtcggtgtag gtctcaccga cgatcttgat
      301 gtccccactg tcgacggccg gcttgatgac cttgtccatg ccactgcgca ggaagtcggc
      361 gttggcgtcg gccttgttgc ccttgatgat gatgtagttg cccttcggca cggcgccgaa
      421 gatcgactcg gcctgcaggc gcccgacctc gacgttgtcg aaggtgatgt agagcgcctt
      481 cgggtcctcg atgaggcggt cgtacgcgat gaccgggacg ccttgctgaa gtgcgctggc
      541 gaccgacggc ttgatggcgg tgccgtcctg ggcgaggatg atcagcacct tggcgccctg
      601 tgagatcagg ttgtcaacgt tgttggcctg ggtctcggcg gacgacttgg cgtcgttcga
      661 gatgtacgtg ccgccacccg ctgcgatcgc agccttgatt gccggctcgt cccacttggc
      721 ccagcgctct tcctggtagt tgttccatga gacgccgacc ttgcaaccgc cggctgctcc
      781 gccgccaccc gctgcggcag tggtggccgc accgcctgcg gcggccgtcg ttgccggggc
      841 ggccgcggcg gtagtggccg cggcagctgc tgtggtggcc gcgcctccgg cggcggccgt
      901 ggtgttcgat gcttcgccgc cgctgctgct gccgcaggcg gccaggacta ccgcagcact
      961 catcgcgagc accactgatc gacgcctatg cattcgggtc tcctcccccg tgtgtcgcaa
     1021 acccacgtgg gcccgcgaga acttgtgcat gttagccaaa cccccttttg gtcggtgact
     1081 tcgtgcctgc gaggccacga cacgcccatg tggcgagcgc gacgacgcaa cctcggcctc
     1141 ctttcgcgag cggtggaacc acgccgctgg atcgtcgagg atcggtccga acgcgagctc
     1201 tgccgcgccg agcagcggcg cgtccactcc gagcgtcgcg ggcaccacgt cgacgagcga
     1261 acgcggagcg gcgagcgcac gtcggtcgag cgcggcctcc acgcgaggtg cgacgaacgg
     1321 atggagccgt gcgaagcgcc caccgagcac gacccgggct ggattgagcg tgttgacgag
     1381 accggagagt ccgagaccca accagtctcc gatctcggcg attgcgccaa gcgcagtggt
     1441 gtcgccgcgg ccagcgtcgt cgagcaccgc ctccaccgcc gcgctgccac cgtcggccgg
     1501 ccggccggcg cgacgcagca gtgcctcctc accgatctcg gtctcccaac agccgactgc
     1561 gccacatcgg caggccgctc cgcccaccgg gttgatcgga acgtggccca cctcgccgcc
     1621 gaagccggcg acacccgtca gcggccggcc accgacgatg agccctccac cgacgccgac
     1681 ctcgcccgac aggaagatca cgtcgtcgac cccgacggcc gcaccgcgtc gatgctcagc
     1741 caaggcaccc agatcggctt cgttggcgac gtggaccggc gccggcgcgc cgagcgcagc
     1801 cgagagctcg acgccgaggg cgacgtcgcg ccaaccgagg ttcggggcga tggacacgaa
     1861 gccgtcgtca cgacggacga caccgacaac ggcgacgccg atccccacga gcgcgtcgtt
     1921 ccgccaccgg gccgacgcgc cgtccgccaa cgcgacgagg tcggcgacga ggtcatccgg
     1981 cgcgaggtgg ccgcgcgacc gatcgacgcg cgcgagctcg agcacctgac cgcccacgcc
     2041 cacgaccgcc atcgccagcg agtcgaccgc gatctcgagt gcgagcacga ccgcgccggt
     2101 ggggtcgagg cggacgacgg gcgagggccg gccgggagat ccctgcgact cgccgcgctg
     2161 ctcgatgacg aggccggccg ccacgagctc gcccaccagt cgtctgatcg cgctgcgcgt
     2221 gagtcccgtg cgctcgacca gctccgagcg cgacatcggc ccttcggcgt ggaggacctg
     2281 gacgagcgcg ctgaggttca tccgccgaat ggtctcgctg cgctgaccgc tgtcggctcg
     2341 acgccccgtc actgaatttg tgcccctcgg gtctaaatgt ttgggacagg gtgctacagg
     2401 tggcgcgcgc tgtcaacgct cagtcgccgg cgtcgtcggc gttcggggcc acgtgcggcg
     2461 ttcaggtcgc tcgtcttagg atgcggccga ccgacgaggc cgccgcgggg gagacgatgt
     2521 gaccagccgg acgctcacgc tcggcaggac cacgtacccc gtgctcctgc cgaacctccg
     2581 cgatccgagg ctgcacgtcg cggccgtcat catcacgatc cacgtcctcg gccaactcgg
     2641 cctccacttc cacgtgagcg tgccccagat cctgtcggcg atcctcacct gcgcgttgct
     2701 ggaggtcgcg ctgacgttcc gggcgcagcg cgccttcgtc tggccggcca gcgcgatgct
     2761 caccgggagc ggcgtcgcac tgatcctgcg cgtgcccgac acgcctctga acgacccctg
     2821 gaccacgcac gcgtggtacg tgttcgccgg cgtcgccgcg ttctcgctgc tcacgaagta
     2881 cgtcatccgc tatcacgggt cgcacgtctt caacccgtcg aacatcggac tggtcgtcgc
     2941 cttcctcgtg ctcggcagcg aacgggtcga gccgctcgac ttctggtggg cgccgctcaa
     3001 cctgtggatg atcaccgcct acgcggtcat cctcgccggc gggctgctag tcacggcgcg
     3061 attgcgcatg ctcgcggtgg ccgtgacgtt ctggctctcc ctcgcagcgg gcgtcgccgt
     3121 cctcgccgcc tccggccatt gcatgacggc tcgttgggcg ttcgcaccgg tgtgcgggtt
     3181 cgacttctgg cgggtgatcg tcatctcgcc cgaggtgctc atcttcctgt tcttcatgat
     3241 cacggacccg aagaccgtgc cgtcgggccg cgtcggtcgg gtcgtgttcg ggctgctcgt
     3301 cgccgtggtc agcacgctcc tgatggcacc gcagacgaac gagttcggca cgaaggtcgc
     3361 gctgctcggg gggttggtcc tcgtgtgcgc agcccggccg gtgctcgacc ggctcgtgcc
     3421 ccagccgcga acggcagccg accggctcgg tcgcttcgcc agccgaatgg cgacgggagg
     3481 cgggtccggc gggtggctgc gaccggtcct gcggattgcc atgatcgctg ccgccgttgc
     3541 cgtggtcggg gccgggatcg tgaccgcggg gacgccggcc cgaggcgtcg tcgtcccggg
     3601 cgtcgacgat gtcgtgggcc gcgtcccgca cgatgtcgat ccggccacgt tccccaagat
     3661 ctcggtcgag caggacgtga tcgactggaa ccacgagatt gccggacccg gcgttcggga
     3721 gatcgccctc acgctcgtcg agaacctgca actggagaac caggcgttgc tccgggccga
     3781 tcccaagatc ctcgaggcgg tcgaccacgg cgaccggctc gacgagatgc gagatcgact
     3841 cgcgcaggcc aagtcgacag ggacgacggt cgtggagcgg tatcagatca aggacgtgga
     3901 cgttgcgctg ctcgtgccgt tcggcaagca ggatggcctg agccttggct tccgctcgcg
     3961 agggctggag acgaaggaga cctacgacag cgatgggcac cttcgggcga gcacgtcgac
     4021 gcccttcgcc aagacgttcg tcgtccggcg ggcgaccggc gcccgctggc tcaatgtcgc
     4081 cgtactcccg ccggcggcgg gcacctgagc gcgtggtcag tgcgtgacgt ccgaacgtcc
     4141 agcttcgctg tggttcaatc cgtcggtgcc cgaggagccg gccgtcgtgc acccggatcc
     4201 ggcgtcgcac cggacgcggc gatcgcgtca ctggccgatc ctcgtcgggc tcgttgccat
     4261 gcttgtcgtc gtcggcggcg tcgtcgtcgc ggtgcgctcg cgatccagtt cctcttacgt
     4321 gccgtcggca ctgcactacg tcgaggaggc ggccgcggcg gggatcgacc accagtacgc
     4381 gggcggctcg cagttcttcg tcggcggcgg tgtggccgcg ttcgactgca acgaggacgg
     4441 acgccctgac ctgttcttcg ccggtggcag cggcccagcc gcgctgtacc ggaacgagag
     4501 tcccaccggc ggcgcgctgc gtttcgcgcg ggtggcgtcg tccgtgaccg atctgacgtc
     4561 ggtcaccggt gcctacccct tggacatcga cggtgacggc cacctcgacc tggcggtgct
     4621 gcgcgtcggc ggcaacgtcg tgctgcgtgg tcggggcaac tgccagttcg agcgagccaa
     4681 cgagcggttc gggattgacg gcgggaacgc gtggacggtt gcgttcagcg cgacctggga
     4741 gggctcgaac gcccttccca cgctggcatt cggcaactac cgcacgcccg acggtgagtc
     4801 gtgcgccgac agcgagctcg tgcgcccagc tgcatccggt gacacgtacg cggcggcgat
     4861 cccactgcac ccgggctact gcacgttgtc ggtcctgttc agcgactgga gcaggaccgg
     4921 caagcgcgat ctgcgcatga cgaacgaccg caactactac cgcgagggag aggagcagct
     4981 ctggcggcta gaaccgggcc aagcaccccg cctgtacacg gcgggggagg ggtggcggcc
     5041 gatgcagatc tggggcatgg ggatcgccag ccaggacgtc accggtgacg gctacccgga
     5101 ggtgttcctg accagccagg gcgacaacaa gctccagacc ctcgcggccg gcgcgactcg
     5161 ccccagctac gcggacatcg cactcgaccg aggcgtcacc gcccagcggc cgttcaccgg
     5221 cggcgacgtg ctgccatcga ccgcctggca tcccgagttc gaggacgtca acaacgacgg
     5281 cgttctggac ctcttcgtct ccaaagggaa cgtggaggcg cagcccgact acgcgaggcg
     5341 cgacccgagc aacctcttcc tcggcgaggc cgacggcacc ttcgtcgagg gggcggaggc
     5401 ggcgggcatc gtccgctacg aacgggcgcg gggagcttcg ctcgtcgacc tgaacctgga
     5461 cgggatgctc gacctcgtcg tggtcgaccg agcggccaac gtccaagtgt ggcggaacgt
     5521 cggacgcggc gacgccgcgc accccgcccc catgggtcat tggatcgccg tcggcctcca
     5581 gcagcccgct ccggacgtgg acgcgatcgg cgcgtggctg gacgtgaagg tcgacggccg
     5641 caccgtcacc cgcgaggtca cggtgggcgg cggccacgca ggaggaaagc tcggttggtt
     5701 ccacttcggt ctgggcgacg cgaaggaggc ccaggtgcga gtcacgtggc ccgacggcca
     5761 gaagggatcg tggatgacga tgcaggcgga tcagatggcg acgatcgagc gcgggagccc
     5821 ggcaccggca cgttggctcc caccccccgc gccatgaacg cgacgcgaac caccggagcg
     5881 gcccgactcg ccgaagtcga gctgcccgac ttcggcatgc ccagagcaga gccgctgtta
     5941 ccagcatccc tgtatgcggc gcggctcgac cgactgcggg cacgcatgga ggcacgcggc
     6001 catgagcaca tcgtcgtctg ggccgaccgt gagcacagcg cgaacctcgc ctacctgacc
     6061 ggcttcgacc cgcgcttcga ggaggcggcg ttgatcgtcg gcagcacctc cgacccggcg
     6121 ctgctcgtcg gcaacgagtg cttcggcatg gccgaagccg cgccgctgcg gacccggacc
     6181 gtgcggttcc aggacttcag cctccccaac cagccgcgcg acgcgtcctg cgcactggcg
     6241 gacatcctcg gcggggaagg catcgccgcg gggcgtcgtg tcggcgtcgt tgggtggaag
     6301 acctacgcca gccgcgacac gatcgagctc ccggcgttcg tcgtcgacgc gctccggcag
     6361 gccaccggct cgcacgcgct cgtggagaac gcgaccgatc tgttcatcga cgcggaggac
     6421 ggcctgcgcg tcgtcaacga ggtcgagcaa ctcgcggcgt tcgagtgggc ggcctgccag
     6481 acgtcgcgcg gcgtgcgcaa tgtcgtgacc ggcgtccaac cggggatgac ggagcgagag
     6541 gcggcccgcc tcctcgagtg gaacggcgcg ccgctgtcct gccacctcat gctgacagcc
     6601 ggaccgagag cgaagctcgg actgctcagc ccgggagacc gaccgatcga acgaggcgac
     6661 ccgctcacca ccgccttcgg catctggggc ggcctcaact gtcgggccgg cttcgtggtc
     6721 gaggatgcag ccgagctacc ggacggcatc cgcgactacg tggatcgtct ggtcgctccg
     6781 tacttcgaag cggtcgccga gtggtacgag gcgctgcacg tcgggcagac gggtggcgag
     6841 ctgcaggcga tcgtggaccg gcacctcggt gacgcgttct tcggcgtctt cctcaacccc
     6901 ggtcaccagc tccatctcga cgaatgggtg aactcaccgg tgttccccgg ctcgacgatc
     6961 gagctgcgat cggggatggc gctgcagtgc gacatcatcc cgacgacggg cacgccgtac
     7021 ttcacgacca acatcgagga tggcctggcg ctcgccgatg actcactgcg cgccgcgttc
     7081 cgcagcctgt acccagatgc gtggggtcgc gtccaggctc gccggcggtt catgtcggag
     7141 gcgctcggca tcgagctgca cccggacgtc atgccgttct cgaacctggc cgcgcacctg
     7201 cccccgttcc tgatgcgacc ggatcgggcg atgacgctcg tgcgctgacc tcagcgggca
     7261 ggcggggcgg atcgcacgag cgccgtgcta gaagcgacgg catgaccgac cagccgaaga
     7321 ccgagcagcc gaacctgcac gactgggcgc cgctggtcga cgacctcgcc gcccgcaaga
     7381 gccgcgccgt ggagatgggc gggccgaagc aggtcgaccg ccagcggtcg atgggcaagt
     7441 tgccggtgcg cgaccgcttg gacctcctgc tcgacccggg gagcttcgtc gagctcggga
     7501 tgctcgccga ctcgatggac gccacgctca cggccgagcg cggctacctg gctgccgacg
     7561 gcatggtggc cgggatcggc gccatcgacg gtcgtcgggt ggcgatctgt gcctacgact
     7621 tcacggtgct cgccggctcg atgggcaaca tcggcgagca gaagacggcg cgcgtgcgtg
     7681 agctcgcgct gcgtcagcgc atccccatcg tgtggctgct ggactcggcc ggcgcccgca
     7741 tccagtcgtc gtcggggtcc acgttcgcgg ctgctggcgc gttgttcaga gagcaggtga
     7801 cgatgagcgg cgtcgtgccg caggtggcgg cgatgctcgg ccactgcgcg gcaggcaccg
     7861 catacatccc ggccctggcc gacttcatcc cgatggtgaa gggcacgtca tcgatggccc
     7921 tcggtgggcg gcacctcgtg cgggcggcca ccggcgagga cgtcaccgag gaggagatgg
     7981 gcggttcgga ggtgcacacc aaggtctccg gcgtggccga cctcgaagtg gcctcggacg
     8041 aggagtgcct cgacgtcgtg cgccggtacc tgtcgttctt cccgtcgcac aacggcgaag
     8101 cgccgcccgt ccggccgacg aacgatccgg tcgaccgccg ggtcgaggag ctctaccggc
     8161 tcgtccccac cgcgccccgc cgtgcttacg acatgaacaa ggtcgtcccg ttgctgtgcg
     8221 acgacggcga gttcttcccg atcaagccgg ccttcgcccg caacctcgtc accgggctcg
     8281 gccggatcgg cggcctgccc gtcggcttcc tcgcctcgaa cccgatggtg ctcggcgccg
     8341 ccctcgacgt gaacgcggcg gacaaagcgg cccgcttcgt ttggctctgc gacgcgttcg
     8401 ggatcccgct cgtgttcctg cacgacgtgc ccggcttcat cgtcggctca gccgtcgaga
     8461 agcagggcat catccgccac ggggcgaaga tgctgttcgc cgtctccgaa gccaccgtgc
     8521 cgaaagtgtc gatcgtgttc cgcaagagct acggcgccgg ctacttcgtg atgaacggca
     8581 ccgcctacga ggcggactac atcgccatct ggccgacggc cgagatcgcg gtcatggggc
     8641 cggacggcat ggtggagatc acgcagcgcc gggtgctcga ccagtacacc gaccccaagg
     8701 agcgggccga cgccaagatc cgcctggccg aggagttccg caagaacatc gacccgtaca
     8761 tcgccgccgg ccacgcggcc gtggacgacg tgatcgaccc ggccgacacc cgcttcgcca
     8821 tctggaaggg tctccaggtc tcgaagacca agcacgtcga gcggccctgg cgcaagcacg
     8881 gcgtcccgcc cgtctgatcc ggtgagacca cccggcagac cactcgtgag accacctgtg
     8941 agaccacctg tgagaccacc cgggagacca cccgtcgcca accgtcgcca cgtttcagcc
     9001 tgacataacg taactccacc ggcgttgggc gattatcggc actctgaggg tgcagttccg
     9061 gtggtgaggg atcccgagcg gcggtcacga ccggcgcgga gaccggcgcg gaggcccagc
     9121 ggaagcccag cggagggcaa gcggagccgg agcggagttc ccgccggtga gtccggtgtg
     9181 atcgacttga ggtgatgggc gagcagtacc tcaccagcaa gctgcagggt ttcgggacga
     9241 cgatcttcgc cgagatgtcg gcgctggcgg tctccactgg ctcgatcaac ctgggacagg
     9301 gctttcccga cgaggacggt ccggccgagg tgctcgacgc cgccctctac gccatcagcc
     9361 acggcaacgg gaaccagtac ccaccggggg cgggcgtgcc cgagctgcgc cacgccgtcg
     9421 tcggccacca gcaccgtttc tatggcttcg agttcgaccc cgacaccgaa gtgctggtca
     9481 cgtgcggcgc caccgaggcc atcgctgccg ccatgctggc actgctcgac accggcgacg
     9541 aggcgatcgc gttcgagccg tactacgaca gctacgcggc gtgcatctcg atggccggag
     9601 cggtgcgccg tccggtcacg ttgcggccgc ccgactacgc cctcgacgtg gacgctctgc
     9661 gcgcggcgat cacgccccga acgaagctga tcctcctcaa caccccgcac aacccgacgg
     9721 gcaaggtgtt cacggccgac gagctggcgg ccattgccgc cgtcgcgcag gagcacgacc
     9781 tcgtggtcgt ggccgatgag gtgtacgagc acctgacctt cgacgacgtg ccccacgtcc
     9841 cgatcgccac ggtgccaggc atgtgggagc gcacggtgac gatcggttcg gcgggcaagt
     9901 cgttcagctt caccggctgg aaggtcggct gggccaccgg gccggcgccg ctgatcacgg
     9961 cggtgcgcac ggcaaagcag ttcctgacct acgtggcgag cggtccgttc cagtacgcgg
    10021 cggcgatcgg gctcggcctg cccgacacgt acttcgagtc ggtacgagcg acgctccagg
    10081 agaagcgcga ccgcctcgcc gacggattga gcgacgccgg gttcaccgtc tttccgcccc
    10141 aaggcacgta cttcatcacg gccgacatcc gagcgttggg cgaggatgac gggatggcgt
    10201 tctgccgatc actgccggac cggtgcggcg tggtcgccat cccgacgatc gtcttctacg
    10261 acgacgtcga cgccggccgg ccgctcgtgc gcttcgcctt ttgcaagcgg ctacccgtgc
    10321 tcgacgaggc cgtcgaacga ctccgttcgc tccggactgg gaggtcagga acatgaggat
    10381 cgcggcgatc cagcacgaca tcgtgtggga ggacggcgac gccacgcgtc gccacgtcac
    10441 cccgatggtg gcggcggcgg ccgcctcggg ggcgaggctc gccgtgctca ccgagatgtt
    10501 cgcgaccggc ttctcgctcg aagccgagcg catcgcggag gccgaggacg gaccgacctc
    10561 gacgtggctg cgcggccagg cggccgagca cggcctgtgg ctgtatggct cggtgccaga
    10621 gcggcggccg gacggcggac gtccccgcaa cgtgggaatc ctcgccggcc cggacggctc
    10681 ggtgcaccgc tacgcgaaga tccacccgtt ctcgtacgcc cgcgagcacg agtcgtacga
    10741 cgcgggtgat cgcaccgtca ccgtcgaggt cgagggcgtc cgttgcagcc tgttcgtctg
    10801 ctacgacctc cgcttcgccg acgactggtg gccgctcgca ccgagcaccg acgtctatct
    10861 ctgctgcgcg aactggcccg agacgcggcg cgcccactgg atcgcgctgc tacaggcgcg
    10921 agcgatcgag aaccaggcct acgtcgtcgg cgtcaaccgc gtcggtgagg gcggagggct
    10981 ctcgtactcg ggcgacagtc gcatcttcga ccccctcgga gagatgctcg ccggcgcggc
    11041 ccggaccgag acgatcctcc tcgccgacgt cgacccggcc gaggtggtca aggtgcggga
    11101 ccggttccgc ttcttgcagg accgccgcac cggagcacct acgtgaccga agtgcgagcc
    11161 gagatcacgg ccaacgtctg gcaggtccgc accgaggtcg gcgcagacgt ggccgaaggc
    11221 gacgagctcg tcatcctcga gtcgatgaag atggaaatac cggtcgccgc acccggcccg
    11281 ggccgcgtcg cggagctgcg ggtcgtcccg gacgacaagg tccaagaggg cgacgtgctc
    11341 gtggtgctcg acccctgacg gagagtggtg acgctggccg cggtaggtgt tggcgcggcc
    11401 gattccaggc caacgtgagc agtggtgcat tgtcgcactg ctcgcgccgg tgctagtcag
    11461 cgctgttcgc cgacggcagg tcggcacgac ggcgagatcc ttcggcccag cagtgcaggc
    11521 cggcgtcata gagagaagcg ggtcaatgag ggtgcggaga ctggcggtcg gcggctgcct
    11581 ggcggttgcg gcaatgacga tgctcgatct ggcgacggct ccgccgcctg cggtcgcggc
    11641 cgtcacgccc gcgttcgtgc aggtgcgggc caacgaggtc agcagcggaa ccacgaacag
    11701 cctcgcgttc acgaacgcgc cgaaggccgg caacctgatc gtcgtctacg tactgtggaa
    11761 caacgccggc gcggtcacgc tgagtgacag ccgcggtgac acctacgcgt ccgccgggtc
    11821 gcgcacgaca tggggcagca actacagcgc gcagacgttc tacgcgaaga acatcgtcgg
    11881 cggcgcgacg accgtcacag cgacgttcgg cacatccctc acgagcttcg ggatcgtcta
    11941 cgtccacgag tactcggggg tcgacaagac caacccgctc gatgtgaccg ccaccgccac
    12001 cggcacggcc aaggcgatga gcagcggcaa cgtcacgacg acgaacgcca ccgatctcct
    12061 cttcggcgcg ggtgcctcga gcaatgccgt caaccagggt ggtaccggct acacgacgcg
    12121 gtcgaccgcc tcgggtaacc gcacagagga ccgggtggtc acatcgacgg ggtcctacgc
    12181 cgcgaccgcg tcgcagaacg gcaatgcgtg ggtgatgcag atcgtggcgt tcaaggccga
    12241 cgcggggggg accggtgaca ccacgccgcc gtcggtcccg accggcctca ccgcgacagc
    12301 ggtgtcccaa acgcagatca acctggcgtg gaacgcgtcg agcgacaacg tgggcgtcac
    12361 gggctacaac gtcttccgga acggcacact cgtcacgacg gttgggacga actcctacca
    12421 gaactccggc ttgagcccag gtacgaccta cacgtacacg gtcagcgcgt tcgatgctgc
    12481 cggcaacacg tccgcgccgt cgacgagcgc gagtgccacg acgcagtcgc cgccgcctga
    12541 catcacgccg cccaccgtgt ccctgaccgc cccggcggcc ggcgcgaccg tgtcggcgac
    12601 gttgtcggtg acggccaacg ccgccgacaa cgtcggggtc gttggcgtcc agttcctgct
    12661 cgatgggacc aaccttggtg gtgaggacac cgccgcgccc tactccacgt catgggacac
    12721 gacgacggcg acgaacggga gccacacgct cagcgcgaag gcacgtgacg cggcgggcaa
    12781 caccaccacc tccgccaccg tctccgtgac cgtctcgaac accgcgccac cgccgggcgg
    12841 tccgcaggcg gcgtacgcct tcgacgaagg ggctggtacg tccgccgccg acgcgagcgg
    12901 tcacggcatc accggcacgc tgacgaacga tgccagctgg acggccggca agtacgggac
    12961 cgcgctcgac ctggacggcg ccggtgactt cgtggacctc ggcaacccga ccgcgttgcg
    13021 gatgaccggc agcatgacgc tcagcggctg gatctacgcg acggcgttcc ccggcgacga
    13081 tgcgcccatc gtctcgaagc gcggctccaa tgggttccag ctggacgtca cgccggacac
    13141 cggcccgcgc acaatcggct tcaagctcac gaacagctcc ggcggagaca tgatccgcta
    13201 cggggccacc gcgctgcagg tcaacagctg gtaccacgtg gtcggcgtct acaacgcgtc
    13261 ggcggtgacg ctgcacgtct acctgaacgg gcagctcgat gacggcgcgc tcgtcggaac
    13321 ggtcactaac tcgcagcaga attcgagcca ggacgtcgac gtcgggcagc gtccgagcgg
    13381 cggcgctttc ttcgacggtc gcatcgacga cgtccgcatc tacaacaagg cgcagagcgc
    13441 ggcggagatc caggcggaca tgctcacgcc gctcggatcc ggtagttcgc ccgacccgac
    13501 tccgcccagc gtcgtgatcg acgcgcccgt gaatggtgcg caggtctccg acatcatcac
    13561 ggtgaccgcc gatgccgagg acaacgtggg ggtcaccggc gtgcagttct tcgtcgacgg
    13621 ccagccggcg ggcgcggagg acacggtcgc gccctacggc ctgcagtggg acacccggac
    13681 cgtctcgaac ggagcgcaca cgctcactgc gcaggcgcgt gatgccgctg ggaacacagc
    13741 gatctccgca ggcgtgaccg tgaacgtcgc caacgccaac ttcttccaga acgaggtgct
    13801 tgccaccggg ttcgaccttc cgaccgccat gaagttcatg ccggacggcc ggctgctcgt
    13861 cgtcgagctg gccggcaccg tgctcatcgt gccgccgccc tacacccagc ccgacccgac
    13921 gccgttcctc cacctgacga acgtcggttc agcaggcgtg cagcagggcc tctacgacat
    13981 cgctctcgat ccgaacttcg cgaccaacca cttcttctac gtcttctaca cgctcggttc
    14041 gccgaaccgc gaccggctct cgcgcttcac cgcgaacgcc acgaacaccg gcacggtcgc
    14101 tggcagtgag ctcgtcctgt accaggaccc tgaggacgcc aacgccgagc accacggcgg
    14161 cgccatcatg ttcggcaacg acggcaagct ctacgtcacc accggcgagc acttcaacgc
    14221 cggggaatcg caggacctca cgaagccgcg ggggaagatc cttcggctga acccggacgg
    14281 caccgtcccg acggacgatc cgttctacga cggcagtggt ccccactggg actcgatctg
    14341 ggccctcggt ctccgcaacc cgttccgcgc ctactacgac tcaccgaccg gtcgcatgtt
    14401 cgtcggcgac gtcggcggaa acgatgcctc gaccgcgaag gaagagatca acatcggcgc
    14461 ccgaggggcg aactacggct ggcccgactc ggagggaccc tgctccggcc agtgcaccag
    14521 cccgctgtat tactaccctc acaacgggcg ggactccgcc gtggtggcgg gcttcgtgta
    14581 tcacggctcg cagttccctt cgtcgtacca gggcagcttc ttcttcgctg actacacgca
    14641 gaactggatc aagcgcctca cgctgaaccc ggatggcacg ctggccaacg tcttcaactt
    14701 cgagcccgcc gacggctcgg tcgacggacc ctacggcgac atcgtctatc tgacccaggg
    14761 ccctgaaggc gcgctctact accttgacct cggctactcg gacatcggag ggacattcgg
    14821 cctcagcaag ctgcgacgga tccgctacat ccagtccgac cagccgccga tcgtcaacgc
    14881 ctccgccaac ccgacgtcgg gcgctgcgcc tctgaccgtc aacttctcga gcgcgggttc
    14941 gtccgacccc gaaggtcacg cgttgacgta ctcctgggac ttcggcgacg gcacgacctc
    15001 caccgcggcc aaccccagcc acacctacac gcaagccggc ccctacgcgg cgcgactgac
    15061 ggtttcggac gggacgaaca acagcatctc tccaccaatc gcgatcaacg tcggcaacaa
    15121 gcccacggcc acgatcacgt cgccgcaaga tggggtgttc ttcaaggccg gtgacgtcat
    15181 ctcgttcagc ggggacgcca ccgacgcgga ggacggcacg ctgcccgcga gcgcgttcac
    15241 ctggaacatc gacttcctgc acgacggcca cgtccacccg ggcgtaccgc agaccggcat
    15301 caagagcggg acgttcacga tcccgaccac cggtcacgac ttcagtggga cgacgcggta
    15361 ccggatcacc ctcaccgtga ccgactcgtc gggcctcacg gacagcaagt tcgtgacggt
    15421 ctggccgacg aaggtcaacc tgagcttcga caccgttccg tccggcctga ccctgaacct
    15481 tgacggcatc gccaagacga cgccgttcgt gtacgacacg ctcgtcggtt tcaaccacac
    15541 catccaggcg cccgaccaga gcgtgggctc gaacaactac acgttcgcct cgtggtccga
    15601 cggcggagcg gcgcagcacg tgatcgtcgt ccccgcgaca gaccaggcgt accgggcgac
    15661 ctacaacgtc acgtcaccgc cacccacgcc agcgttcgtt cagatcacga gcaccacgcc
    15721 gcagagctcg ctggcgactg tgacgctcgc gtatcccggt gggcaggtcg gtggcgacac
    15781 caatgtgatc gcggtcggct ggaacgacgt gtcgtccacg atcacgtcgg tgaccgacag
    15841 cgccggcaac acctatcagg tcgccacgcc gatggcgcgc gggcccgccg tgagccaggc
    15901 gatctactac gccaagaaca tcaaagccgc ggctgcgggc accaacacgg tgaccgtgcg
    15961 attctccgct gctgcaccct tccccgacat ccgcgccgcc gagtacagcg ggctcgatct
    16021 cctcaacccg ttcgacaaga ccgggtcggc ctcggggacg agcgcgacgg cgacgagcgg
    16081 cagcgtgacc acggcatcgg cgacagagct gctgttcggc gcgggcatga ccaccggtgt
    16141 gttcaacggg tccgccagtg gctacacgac gagggtgatc acgccgcagg acggtgacat
    16201 cgccaacgac cgcaacgtca cggcggttgg cagctacacc gctggggcga cgctcagcgg
    16261 gtcggcgggc tggctcatgc agatcgccag cttccgggcc gcgggccagt agccgcccgc
    16321 cccggtgctc ccaagctgcc gctcagatgt tcggtggcag gagccggttg acgttctcga
    16381 agaagaagta gaggcacacg acgaagggcg cgacggcggc gatcaccccg acccaccggc
    16441 ggcccgtcca gcgtctcaag ccccagccga ggagcgagca ggccaccacg agcaggcccc
    16501 agcagaccac cggcccccat gccgaagagt cggcgaacca gcccttctcg agcgcgtcgg
    16561 gggtgtcacc ggcgatcggt tgatcggcga cggacggcgg cgttggtgca agcgctgcgg
    16621 ccgtggtcgc aggaggggcc gaagcggcag aggtggtggt cgacgcggca ctctgcggcg
    16681 cggcagtcgt cggggcgatc gttgacggtg caacagtcgt cggtgccacc gtgggcggcg
    16741 gcgccgtggg ggcgagcggc gaggacgccg ccgcgtcgag cttcgccttg atgatgatcc
    16801 gcttcctcgc cgagtagcgc ggatggcacg acgtcagcgt cagctccgcg tccggggtcg
    16861 gggccaggac ctcggactgc gacggcgaca cgatgaactg ctcggtgacc ttgtacacgt
    16921 accggccagt cgccgtggtg aggatcacgt cgtcgccggg ctccagctcg ttgatgcgga
    16981 agaacggatg gccgaacgtc gtgcggtggc cggcgatgcc gacgttgccg atgtggccgg
    17041 gcagcggcgt gtcggggtag tgacccggcc ccttcttcag gtcgtccacg ccgacgccgt
    17101 tgacgacgat ccagttcagc ccgatcttcg gcgcttcgag gcgggcgatc ggatctcccg
    17161 gcgcaggtgc ggcggtggtg ggcggtggag ccggcgcagt cgtggcgacg gtgtcaggca
    17221 gcgtcgacga ggagctcgac gggaccgacg atgcgaccga cgacgaggac gatgacgacg
    17281 tcgacgtcgg cgccaccgtg gtcgtcgtgg tggcgagcac gtcgtcgaac tggttggaga
    17341 gctggttctg tgactggcgg gtctggatcc ctgtgcctca cagctggtag gcgacgaagg
    17401 ccaggagcag cagccccgtc acaacgagga tccgaccgac gaggcccacc caccagcgcc
    17461 agtccttcct cgggtgcggc ggtggggcct tctccgacgg cagcggcggg tcccagtccg
    17521 ccgatgtcgc ctcgccgttc acgcaggcga cggtaccggc ccgaccccag gttgcggcgt
    17581 gcggccgcct ggttgcattg tcgtcgcgcg cggcggatca gtaccgttgg cccggcgtat
    17641 ggaaccggtg atccacctcc gcgacgccgt ggcggtgctg ggccggttcc cggccctggc
    17701 tggcgcgtcg ctgacggtcg agcgaggcga gatcgtgctc ctgaaggggc cgaacggcgc
    17761 cggcaagacc accttgttgc ggttgtgcgc cggcttgctg ccgctcgagc ggggtgaggc
    17821 ccgcatcctc ggtgtcgacc tggccaccga ccgtcgccgc gtgcgggccc gggtcggtct
    17881 gctcggtcac agcaacggcc tgtacgacga tctgtcggtg gccgagaacg tgcgcttctg
    17941 ggcccagacc gtcggcgcga ccagcgcgga ggtcgacgct gcgttggctc gtctcggtct
    18001 cgcgggacgg ctcgccgaga cgaaggtggc gcgcctttcc gcgggccagc gacgtcggac
    18061 ggcgctggct gcgctcgtcg cccgccggcc cgagctgtgg ttgctcgacg agccacacgc
    18121 cggtctggac gccgagggcc gcgacgtgat cgacggtctg ctccacgccg cggtcgccgc
    18181 cggcgccaca gtcatggtgg cgtcgcacga acgggaacgg gccggtgcgc tcgcccaccg
    18241 ccgggtcctc gtcgtcggcg ggcagatcct cgacgacggg ccgcacgccg cgggcgggag
    18301 gatggcccgg gccgtcgacg agcagcgcgt tgccagctcg ccggccggcg ccgcgtcgcc
    18361 gaccgagccg gcaccggccg gggccacgcc gtgctgagga cgctgcgcct cgtcgccggc
    18421 aaggacctgc ggatcgaggc tcgtagccgc gtggcggcga accaggtcct gcccctcgcg
    18481 gtgctcgtgc tcgtgctgtt cgccttcgcc ctcgaccccg acagcggcgt gctccgtcgc
    18541 gccaccccgg gcctgttctg gctcaccgtg ctgttcgccc tggtgttcac ggtccagcgc
    18601 tccttcgcgc tggagacggc ggacgacgcc caggacatgc tgcggctcag cgcgctcgag
    18661 ccggctggga tcttcctggg gaaggcggcc gcgctcctcg ccgaactgct gctcttggag
    18721 ctcggcttgg gcgtcgggat catcgtcctc tacgacgccc cgacccgggc cgcgggcgtc
    18781 gttctgctcc tcgtagcggc cgtggccgcg acctgcggtc tggccgcggt cggtacgctc
    18841 tacggcgggc tcgctgccgg gctccgggtt cgggacacgt tgctcccgct gctcctcctc
    18901 ccggtggtgg cacccgtgct catcggtgcc agccgggcgt tcgaagccgc tctcggcatc
    18961 ggcgataggg cgattagcga gggctggccg tgggtagggc tgctcacggt ctttgcgctc
    19021 gcctacaccg ccctcggtct ggtggtcttc gggccgctca tggaggagtc atgaccacag
    19081 gaggtgcgac agcggccgag acgggaacgg tgagcgtcgc gccgacgagc gatgcaccgc
    19141 acgccgatag tccggcccgc cgcaccagcg cgaccggtac caaggccact cgggtcatcg
    19201 gcgggctcgc gctcgccggc atcccgctgc tgttgctgct cgggctcgtc acgtcgcccg
    19261 aggacgtcga gatgggcgac agcgtccgca tcatgtacgt ccacgtgcct tcggcctggc
    19321 tggcgtacct ggcgttcggc gtcaccgccg tgggctccgc cggttacctg tggagacgca
    19381 cccggtcgct ggcgtgggac cggctcgccg gtgcgtcggc ggagatcggc gtgctgttca
    19441 ccggtctcgc gctcgccacc gggatgctgt ggggccgtcc cacgtggggc gtct
//
LOCUS       DATBFZ010000073         8296 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_377800, whole genome shotgun sequence.
ACCESSION   DATBFZ010000073 DATBFZ010000000
VERSION     DATBFZ010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 8296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8296
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377800"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..800)
                     /locus_tag="VH419_02230"
     CDS             complement(<1..800)
                     /locus_tag="VH419_02230"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY7042465.1"
                     /translation="MKRVRLVVGLIGVAVLMLSACTSSDDSSSSGKVVLKLWGENEGG
                     VGPFLDRLESSFEDAYPNIDLQITNFPEQNYDVKLQTAIAAGKEPDLVQAGGDLEAMR
                     TGKLLSLDDMVKQHHINLSHYVQSIVEPGDEFSCNYEGHLYCIGSYAGSVQLFYNKDM
                     FDAAGIPYPKAWPPMTPDEFVSIACQLTDKDKQVWGAAASDPLAYVPWEVFFSSDGRT
                     ATFNSPEAVHQVEVLADGYKNGCFPTSNVIDPWEQGRDYFIKGQLAMV"
     gene            998..1429
                     /locus_tag="VH419_02235"
     CDS             998..1429
                     /locus_tag="VH419_02235"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY7042466.1"
                     /translation="MKAAEPPDPGTGEVQTASDGDIVIAQRLGPDQRPTWSDVTSAGI
                     FRVPPAGRFDQHYHDCDEYWLVFAGRARVSVGDRPYDVGPGDIVCTPAGTDHDVIGVQ
                     ETLQAFWFEGRTPAGGRVGHLHRTPADAEGHSVPPLPDSEK"
     gene            complement(1464..2765)
                     /locus_tag="VH419_02240"
     CDS             complement(1464..2765)
                     /locus_tag="VH419_02240"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEY7042467.1"
                     /translation="MWDALDPRVMVAEELVERRQAGFDVSGVEDVVRDALEDGSAEAI
                     EQVYVALELTQLRPGWLFDEPSTLDEIRSALPDTPVVPPHRQSHDEVRDRLLGAWLGR
                     CVGCNLGKPVEGWSRERIRRYLELADAYPITDYIPVLEPAPADLQLNPCWRETTRGNV
                     ACMARDDDIDYTILGLHVLESWGFGFGTEQVAAEWLDHLPFTQVYTAEQVAYRNLVHR
                     IRPPETASHRNPYREWIGAQIRADVWGYVSPGDPGRASELAFRDASLSHTQNGIYGEM
                     WAAALIACAFVAGDAESALELSLAFVPARSRLAAALGSVLGLHADGLGWEQARDAIED
                     DLGIYGRVHTINNAALVAAGLLWGAGDFTRTIGLAVQGGWDTDCNGATAGSVFGALHG
                     TGALPGHWVDPLHNLVRSSINGFDNSAVSDLAERTFRLAAG"
     gene            complement(2816..5311)
                     /locus_tag="VH419_02245"
     CDS             complement(2816..5311)
                     /locus_tag="VH419_02245"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HEY7042468.1"
                     /translation="MTNEASVDYLTLDLTAYAAAGPDALGDAGRIGGGLRTFHGIPFQ
                     IGPTPDRPSFVLLKPGEPTVDIPVGERARRVVVAHLRLPVPAASAPELGDVVAEYTFE
                     TEGGGSPAVPVREGFEVAAPPDGWGWQGPFLAVAARTGESLDRRRGAWDEAGVRQTET
                     PFRQTTTELFLWTWKNPHPDSTVTSIRLRARTCPLIVAAITLGTGDEDPFVREAAVPV
                     RVTVRDADRPEVNCDWTFVVDRGVTTYGYPLPARALADYLDDPLKGWGEAPDSTSGRA
                     YARVAAVPSATLAIGRGQQTLARLPWREVTTAGSAERDGVRVELVEPGRNWVHVRVLD
                     DDTGRPVPCRVHFRSPDGVPYQPHGHHDHVNSDLGTWHVDVGGDVRLGRLTYAYIDGT
                     CQGWLPVGDVVVDVARGFEYEPLRAVVQIRHGQRELELRLRRWTSMNERGWYSGDSHV
                     HFLSAQGALTEQQGEDLNVVNLLQSQWGSLFTNTEDFTGRHQATNDGQYVTWVSQENR
                     QHMLGHLVLWGLKSPVMPWCTDGLSEAEYGGALEATESDWADQCHEQGGTVVIPHFPL
                     PNGEPATLIATGRADAVEMIVQHPGFHREYYRYLNGGYRLPLVGGTDKMSSEVPVGLY
                     RTYVRLGDEPFSYDAWRKGVVLGRTFLSGGPLLSLTVDGADIGDTVRLRGAGTVSVRA
                     TADSVFPISTLEIVRNGEVVAATSDGSVRRLEVSADISVDGDCWLAARCGGPSHFDGP
                     VHRDVWGRGVFAHTSPVYVATQGDEYGLFDETHARYMLTLIEGSLGYIRQRAARYPHS
                     QVTHHHGENDHLAYLERPFLQAREVLERRLDEG"
     gene            5432..6562
                     /locus_tag="VH419_02250"
     CDS             5432..6562
                     /locus_tag="VH419_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042469.1"
                     /translation="MPAPSQPDGPGMIVQRGFSTRPARSPAVALALTSLAASGIHFAV
                     MADHFAEAFVFGLFFAVVAWAQALWAVAVVVAPVRIVLTSGIIGNLAVIAVWAMSRTV
                     GVPLGPEPWTPESIGPADLLCGVLEGAIVLGCVLLLRHDMADATPDQDRRAVVALPAL
                     LAFGVALAVGTSVAIAASPEHEHAEAGHSHAAEHTASSHDGHVLVGGNGEPDLTQIAV
                     IRHAMRKYLDVRDARAAGWEQEHRDWPELGAHFARNSDWDGSFPARPGLRIRQPEFLM
                     YSRYLTGHWKLVAVAYVFDQALYPQPPKGLVGAQYHQHAWNCIKNGDELEEEDWGVLS
                     REECKIMRGVWSPGGVWMTHVWLVGNPDGIFAATNPRLTKLR"
     gene            complement(6584..7156)
                     /locus_tag="VH419_02255"
     CDS             complement(6584..7156)
                     /locus_tag="VH419_02255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042470.1"
                     /translation="MDRSTHLKSSCRVAAVVGTAALLTAGVQYHVLADHDHSRAARSE
                     VSAALGADRIVALRESTFASTQSRIYRELASTSLTVPSGQHGLTVAHFGAESLCPGAA
                     GSGYCSIRIRMDGTTEMVPQSGTNFAFDSASRDGRESHSMERTISGVDAGTHTFSVEW
                     RLVCDPITHKCPGSFKLDDWVFDVEFWRQS"
     gene            complement(7332..8042)
                     /locus_tag="VH419_02260"
     CDS             complement(7332..8042)
                     /locus_tag="VH419_02260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042471.1"
                     /translation="MRGRTDTEVAASPVASSRAQTWARNWWLVPAVLAAALAALFMVV
                     MPPHRTLNSVLEVPWRLSPVLLAVVAIAGFPQRRGLAMTAVGATFIAYMGVVDTSLVL
                     QVDDYVAAGSSAQDAFDSFYQFQLFMSAFMVLAIVLAYRLGGGGTTRTLQVGTAGVLV
                     LISGLNDLSYWLLADWPNGRPDTLFWASHIKVFVGESPSIPVAIVFTTVHLVLAGIVL
                     TPPVERRLDRLAARLGTR"
     gene            complement(8061..>8296)
                     /locus_tag="VH419_02265"
     CDS             complement(8061..>8296)
                     /locus_tag="VH419_02265"
                     /inference="COORDINATES: protein motif:HMM:NF028171.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HEY7042472.1"
                     /translation="AENPKYHAMISHFDRLTGVPMVTNTSFNDNEEPIVASPADAIRC
                     FLGTGMDTLALGDFWIDKATFGEAGGGADVAAG"
BASE COUNT         1381 a         2763 c         2763 g         1389 t
ORIGIN      
        1 atcaccatgg cgagctgacc cttgatgaag tagtcacggc cttgctccca cgggtcgatg
       61 acgttcgagg tcgggaagca gccgttcttg tagccgtcgg ccagcacctc gacctgatgc
      121 acagcctccg gcgaattgaa cgtcgcggtg cggccgtccg agctgaagaa cacctcccag
      181 gggacgtacg ccagcgggtc ggaggcagct gcgccccaga cttgcttgtc cttgtcggtc
      241 aactggcagg cgatcgagac gaactcgtcg ggtgtcatcg gtggccacgc ctttggatac
      301 gggataccag cggcgtcgaa catgtccttg ttgtagaaga gctgaacgga tccggcatac
      361 gagccgatgc agtacaggtg gccctcgtag ttgcagctga actcgtcgcc aggctcgacg
      421 atcgactgga cgtagtggga aaggttgatg tggtgctgct tgaccatgtc gtcgagcgac
      481 aggagctttc ccgttctcat cgcttcgagg tcgccgccgg cctgaaccag atccggctcc
      541 ttgcctgcgg cgatcgcggt ttgcagtttc acgtcgtagt tctgttcagg gaagttggtg
      601 atctggagat cgatgttcgg gtatgcatcc tcgaaggagc tctccagccg atcgaggaac
      661 gggccgactc caccttcgtt ctccccccag agcttcagca caaccttgcc gctgctgctc
      721 gagtcgtcgc tcgaggtgca ggcgctcagc atgagcaccg caaccccgat cagtcccaca
      781 accagccgga ctcgtttcat cgtcggacct tcctcgcgcc aggccgggtg taccgacagg
      841 tcggctgaca gcaggttctc gccgaccgct cgataaccgc tgatggcgcc gccggaacgg
      901 agccagagcc gaatttatgg taacgttctc aggatctgag cgtcaatgta cgcccgtcca
      961 ggtaatccag cggcccacaa cgatgggagc actccggatg aaagcggcgg agcctcctga
     1021 ccccggcaca ggagaggtcc agaccgcgtc tgacggcgac atcgtcatag cccaacggct
     1081 cggccccgac cagcgcccga cctggtccga cgtcacgtcc gcggggatct tccgagtccc
     1141 tcccgctggg agattcgacc agcactacca cgactgcgac gagtactggc tggtctttgc
     1201 cggccgagct cgggtgagtg tcggcgaccg gccgtacgac gtcggaccgg gggacatcgt
     1261 ctgtacgccg gcggggaccg accacgacgt gattggcgtc caggagacgt tgcaagcgtt
     1321 ctggttcgaa gggcgaaccc ctgccggcgg acgcgtcggg cacctccacc ggacaccagc
     1381 cgacgccgag ggccattccg tcccgccgct gccagattcg gaaaagtgac cgcccggccc
     1441 agccacgtca ctgcggcata tgatcagccg gcggccaacc ggaaggtccg ctcagccagg
     1501 tcggagacgg cggagttgtc gaagccgttg atcgagctgc gtacgaggtt gtgcagcggg
     1561 tcgacccagt ggccgggcag cgccccggtg ccgtgcaggg ctccgaacac ggaccctgcc
     1621 gtcgcaccgt tgcagtcggt gtcccacccg ccctggacgg caagcccgat ggtccgcgtg
     1681 aagtctccgg cgccccagag tagtccggcc gcgaccagcg ctgcgttgtt gatcgtgtgg
     1741 acgcgcccgt agatcccgag atcgtcctcg atcgcgtctc tggcctgttc ccagccgagt
     1801 ccgtcggcat ggaggccgag cacggagccc agtgcagccg caagccggga ccgggcaggc
     1861 acgaaagcga gagagagctc cagggcagat tccgcgtcac ccgccacgaa cgcgcacgca
     1921 atcagggcag cggcccacat ctccccgtag atgccgttct gcgtatgcga caagctggca
     1981 tcccgaaacg cgagttccga cgcccgccct gggtcgcccg gggacacgta cccccacacg
     2041 tccgcccgga tctgcgcgcc gatccactcc cggtacggat tgcggtggct ggccgtctcg
     2101 gggggccgga tccgatgcac gagattgcgg taggcgacct gctcagctgt gtacacctgc
     2161 gtgaacggca agtggtcgag ccattcggca gcaacctgtt ctgtgccgaa gccgaaaccc
     2221 cagctctcca aaacgtgtag gcccagaatc gtgtagtcga tgtcgtcgtc gcgcgccatg
     2281 caagcgacgt tgcccctggt cgtctcgcgc cagcacgggt tcagctgcag atcggccggc
     2341 gccggctcca gcacggggat gtagtcggtg atcgggtagg catccgccag ctcgaggtat
     2401 cggcgtatgc gctcccggct ccagccttcg accggtttgc cgaggttgca gccgacgcag
     2461 cgtccgagcc atgcgccgag cagccggtcg cggacctcgt catgcgactg ccgatggggc
     2521 gggacaactg gcgtgtcagg cagagcgctt cggatctcgt ccagcgtcga tggctcatcg
     2581 aaaagccagc cgggccgaag ctgtgtcagc tcgagcgcca cgtaaacctg ctcgatcgct
     2641 tccgcggacc cgtcctcgag cgcatcacgg accacatcct cgaccccgga tacgtcgaac
     2701 cccgcctgcc ggcgctcgac cagttcctcg gcgaccatca cgcgcggatc gagtgcgtcc
     2761 cacatggcct tgcctcctca tgcccgtgtc ggtgcagcat ccggcgtggt gtcgttcaac
     2821 cttcgtcgag acgtcgctcg agcacttcgc gtgcctggag gaatgggcgc tccagataag
     2881 cgagatggtc gttctccccg tggtggtggg tcacctgcga gtgcgggtac ctggccgccc
     2941 gctgccggat gtagccgagg cttccctcga tgagcgtgag catgtagcgg gcatgcgtct
     3001 cgtcgaacaa cccgtactcg tcgccctggg tcgcgacgta caccggcgag gtgtgcgcga
     3061 agacaccacg gccccagaca tcgcgatgta cagggccgtc gaaatggctc ggtccaccgc
     3121 accgtgcggc cagccagcag tcgccgtcga cactgatgtc cgcggacacc tcgagtcgtc
     3181 gaacgctccc gtcgctcgtc gcggccacga cttcaccgtt ccggacgatt tcgagggtcg
     3241 agatggggaa cacgctgtcg gccgtggctc tgaccgagac ggtacctgca ccgcgaagcc
     3301 gaaccgtgtc gccgatgtcc gccccgtcga cagtcagcga gaggagaggt ccgccgctga
     3361 ggaaggtccg ccccaggacg acccccttgc gccacgcgtc gtacgagaac ggctcgtcgc
     3421 cgagccggac atacgtgcgg tagagcccga ccggaacctc ggacgacatc ttgtccgttc
     3481 caccaacgag ggggagccgg tagccgccgt tcaagtagcg gtagtactcc cggtggaaac
     3541 ctggatgctg gacgatcatc tcgaccgcgt cagcacggcc ggtggcgatc aacgtcgcgg
     3601 gttcgccgtt gggtagcggg aagtgtggga tgacgaccgt tccgccctgc tcgtggcact
     3661 ggtcggccca gtcgctctcc gtggcctcga gcgctccgcc gtactcggct tcagacaacc
     3721 cgtccgtaca ccacggcatc acgggactct tcaaacccca cagcacgagg tgaccgagca
     3781 tgtgctggcg gttctcctgg ctcacccacg tcacgtactg accgtcgttg gtcgcctggt
     3841 ggcggccggt gaagtcttcg gtgttggtga acaggctgcc ccactggctc tgtaggaggt
     3901 tcacgacgtt cagatcctcg ccctgctgct cggtgagcgc tccctgcgct gataggaagt
     3961 gcacgtgcga gtcgccggag taccagcctc gttcgttcat cgaggtccag cgcctcagcc
     4021 ggagctcgag ctcgcgctgc ccatgccgga tctgcacgac cgcgcgcagc ggctcgtact
     4081 caaaaccgcg tgcgacgtcg acgacgacat cgccgaccgg cagccaaccc tggcacgtcc
     4141 cgtcgatgta ggcgtaggtg agccggccga gccggacatc accgccaacg tcgacgtgcc
     4201 aggtgccgag gtcggagttg acgtggtcgt ggtggccatg gggctggtat ggcacgccgt
     4261 cgggagagcg gaagtgcacc cggcacggca ccggccttcc ggtgtcgtcg tcgagcaccc
     4321 ggacgtgaac ccagttccgg ccgggctcga cgagctcgac tcggacgccg tcgcgctccg
     4381 ccgacccggc ggtcgtgacc tcccgccacg gcagccgcgc caaggtctgc tggccgcggc
     4441 cgatcgccag cgtcgccgac ggaaccgcgg caacccgggc gtacgcccgg ccactggtcg
     4501 agtccggtgc ctcgccccag cccttgagcg ggtcgtccag gtagtcggcg agcgcacgcg
     4561 ccggcagcgg gtacccgtag gtcgtgactc cgcggtcgac gacgaacgtc cagtcgcagt
     4621 tcacctcggg ccggtccgcg tcacgcaccg tcacgcggac ggggaccgcg gcttcccgca
     4681 cgaacgggtc ctcgtccccg gtccccaggg tgatcgcggc gacgatcaac gggcatgtgc
     4741 gggccctgag acggatcgaa gtgacggtcg agtccgggtg cggattcttc caggtccaca
     4801 agaagagctc ggtggtcgtc tgtcggaacg gcgtctcggt ctgccgcact ccggcctcgt
     4861 cccacgcccc gcgacgccga tcgagcgact cgcctgtgcg ggcagcgacc gccaggaacg
     4921 gaccctgcca accccagccg tctggcggtg cggccacctc gaacccctct cggacgggaa
     4981 ccgccgggct gccgccaccc tccgtctcga acgtgtactc ggccaccacg tcgccgagct
     5041 cgggcgccga cgccgcgggt acggggagcc gaaggtgtgc gacaaccacc cgccgagccc
     5101 gttcgccgac ggggatgtca acagtgggtt ctccgggttt gagcaggacg aacgatggac
     5161 ggtctggcgt cggtccgatc tggaacggta tgccgtggaa ggtccgaagg ccgccaccga
     5221 tccggccggc atcgccgagc gcgtcgggcc cagccgcggc gtatgccgtc agatcgagcg
     5281 tgaggtagtc gacggacgct tcgttcgtca tcgatcgtct ctccggcgtc gaaccgtcgg
     5341 gcaggcaggt cgaatcgacc cagccgatgc tcgcaggtcg cgcggttccc ctccctggct
     5401 gatgggttat gggaacgtta tcatggggaa gatgccggct ccttctcaac cggacgggcc
     5461 aggaatgatc gttcagcgcg ggttctccac caggccggcg cgatcgcctg cggtcgctct
     5521 tgcgctgacg tcgctcgccg catccggaat ccacttcgcc gttatggccg accacttcgc
     5581 ggaggcgttc gtcttcggcc tcttcttcgc ggtggtcgct tgggcgcagg cgctgtgggc
     5641 cgttgccgtc gtggtggcgc cggtccgcat cgtgctgacc agcgggatta tcggcaacct
     5701 cgcggtcatc gctgtctggg caatgtctcg cacggtcggc gttcccctcg ggccggagcc
     5761 ctggacgcct gagtcgatcg ggccggctga cctactctgt ggcgtgctcg agggcgcgat
     5821 cgttctcggt tgcgttctcc tgctccgtca cgacatggct gacgcgacgc ccgaccagga
     5881 ccgccgagcg gtcgtcgcgc tgcctgccct gctggcattc ggtgtcgcgc tcgcagtcgg
     5941 cacgagcgtg gccatcgccg ccagcccaga gcacgagcat gccgaggcgg gacacagtca
     6001 tgcagctgag cacacggcga gcagtcacga cgggcacgtc ctggtcggtg gcaacggcga
     6061 gccagacctg actcagatcg ccgtcatccg tcatgcgatg cgcaagtacc tcgacgtccg
     6121 ggacgcccgc gctgctggct gggagcagga gcatcgcgac tggcctgagc tcggcgcgca
     6181 cttcgcccgc aacagcgact gggacggatc gttccctgct aggccgggcc tgcggatccg
     6241 ccagccggag ttcctgatgt actcgcggta cctgaccggg cactggaagc ttgtcgcggt
     6301 tgcctacgtg ttcgaccagg ctctgtaccc ccaaccgccg aagggccttg ttggcgcgca
     6361 gtaccaccaa cacgcctgga actgcatcaa gaacggcgac gagctcgagg aggaggactg
     6421 gggcgtcctc agccgtgagg agtgcaagat catgcgtggt gtctggtccc ccggcggtgt
     6481 ctggatgacc cacgtctggt tggtcggcaa ccctgacgga atcttcgcag cgaccaatcc
     6541 ccgactgacg aagctccggt gatcgcgcag gctcaggtcg tggtcacgac tgacgccaga
     6601 actcgacgtc gaagacccag tcgtcgagct tgaaggaccc ggggcacttg tgggtgatgg
     6661 ggtcgcagac caacctccat tcgaccgaga aagtgtgggt gcctgcgtca accccgctga
     6721 tggtgcgctc catcgagtgc gactcccggc cgtcccggct ggcgctgtcg aacgcgaagt
     6781 tagtgccgga ctgcgggacc atctccgtgg tgccgtccat tcggatgcgg atcgagcaat
     6841 agcccgatcc cgcggccccg gggcaaaggg attcggcgcc gaagtgggca accgtgagcc
     6901 cgtgctgacc tgacggcacg gtgagcgagg tcgaggccag ttcccggtag atacgtgatt
     6961 gcgtgctcgc gaatgtgctc tcccgcagcg ccacgatgcg gtcggctccc aaagcggccg
     7021 acacctcgga ccttgcggca cgcgagtggt catgatcggc caggacgtgg tactggacgc
     7081 cggccgtcag cagggccgcc gtaccgacga ctgcggcgac acggcatgag ctcttgaggt
     7141 gggtggaacg gtccatgatc tctcctgttt actgcgtcgt ttgtgggtga agtggctctt
     7201 gtcaccgagc gtccgggtaa gagcagcaga gatcgtcggc cgaaatgctt attcttggca
     7261 gggatgccgc agacgcggac cttcacggtc ttcgggcaaa ggactcggcg aacgcgatgt
     7321 acgacgaggg ctcagcgcgt acctaggcgg gcggcgagcc ggtcgagccg acgttccacg
     7381 ggtggcgtga ggacgatccc ggcgagaacg aggtgcaccg tcgtgaagac gatggcgacg
     7441 ggtatggagg ggctttcgcc cacgaacacc ttgatgtgcg acgcccagaa cagggtgtcg
     7501 gggcggccgt tcggccagtc cgccaggagc cagtagctga gatcgttcag cccggagatg
     7561 agcacgagga cgccggccgt gccgacttgc aacgtgcgcg tcgttccacc accaccgagg
     7621 cggtacgcga gaacgatcgc caggaccatg aaggccgaca tgaacagctg gaactggtag
     7681 aagctgtcga atgcgtcctg ggcagagctg cctgcggcca catagtcgtc gacctgcagc
     7741 accagcgatg tgtcgacgac acccatgtac gcgatgaacg tggcgccaac ggccgtcatc
     7801 gcgagcccgc gacgttgcgg gaacccggcg atcgcgacca cagcaagtaa cacggggctc
     7861 aaccgccacg ggacctcgag caccgagttg agtgtgcggt gcgggggcat gacgaccatg
     7921 aacagtgctg cgagtgccgc cgcgagaacc gccgggacca gccaccagtt ccgagcccag
     7981 gtctgcgccc ggctcgacgc caccggggac gcggcgactt cggtatcggt tcgaccccgc
     8041 atcacgagga ggtctcctca tcacccggcc gcgacatcgg ctcccccacc ggcctcgccg
     8101 aacgtggcct tgtcgatcca gaagtcgccg agcgcaagcg tgtccatacc ggtgccgagg
     8161 aagcaccgga tcgcgtccgc gggcgaggcg acgatcggct cctcgttgtc gttgaacgat
     8221 gtgttggtga ccatcggtac cccggtgagc cggtcgaagt ggctgatcat ggcgtggtac
     8281 ttggggttct ccgcct
//
LOCUS       DATBFZ010000074         3211 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_5276002, whole genome shotgun sequence.
ACCESSION   DATBFZ010000074 DATBFZ010000000
VERSION     DATBFZ010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 3211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3211
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5276002"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            156..461
                     /locus_tag="VH419_02270"
     CDS             156..461
                     /locus_tag="VH419_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042473.1"
                     /translation="MTDQDVAETARRAHRRSGMLWGARVISYIMLAAVALTGAYSAGN
                     KAYASAHCSGPASSLGECDLADFAGLEWATIAFIGTVTLIVAVEVMLWLRRRSRATA"
     gene            823..1077
                     /locus_tag="VH419_02275"
     CDS             823..1077
                     /locus_tag="VH419_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042474.1"
                     /translation="MNRPPQVQDHRPLLEAAGFEVERYDDTDDWEARQRATTRALLDR
                     VEDLALESGQSPDDLRVGIAEMAATFDCMIRRVLIVARRA"
     gene            complement(1165..1467)
                     /locus_tag="VH419_02280"
     CDS             complement(1165..1467)
                     /locus_tag="VH419_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042475.1"
                     /translation="MSTLIAVRLPDEMAAFLDRTVATGKAPSRAVLVAAAVEREMRRQ
                     AAEQDAQVLREKGPADGWTIWCSGAAPTSRYPFSHDLHARPQRHSGRSCAGVSVVS"
     gene            1775..3048
                     /locus_tag="VH419_02285"
                     /pseudo
     CDS             1775..3048
                     /locus_tag="VH419_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011691690.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
BASE COUNT          569 a         1123 c         1018 g          501 t
ORIGIN      
        1 tcgtacctcg ggccgcgacg agcatgctgc gcctagcgat caatgcatcg caaccgcact
       61 catctgccgc ggtccgtgtg cggttcatcc ggtgagcggg agatgaatac gagactgcat
      121 gcgcctgagg agtagcgaac cgcatacttt gcgacgtgac agaccaggac gtcgccgaga
      181 cggcgaggcg tgcccaccgt cgcagcggga tgttgtgggg cgctcgcgtc atctcctaca
      241 tcatgttggc ggcagttgcg ttgacgggcg cctattccgc agggaacaaa gcgtatgcct
      301 cagcgcactg ctcgggtccc gcgtcgtctc taggcgagtg cgacctggcg gacttcgcgg
      361 ggcttgagtg ggcgaccatc gccttcatcg gaacggtgac tctgatcgtg gccgtcgagg
      421 tcatgttgtg gcttaggcga aggtcacgag cgacggcgtg actccccaga accgaccgct
      481 catcagcgtg aggcgttttg cgtcaagtgc tgggtgggtc tctggctcgt cgtcggtccc
      541 tgcatagcgg tatcggagct ggtgctcggc agcgtgtgat cgggttgtgt cgacgacggg
      601 ccgggacggc ctgtgaagag cgacgagata cctcggaggg ctctcaaatg ccgcggaagg
      661 ctcgccccgg cccagctttg tggacgcgcg acgcatcgcg cgatggggtg gttcaggcgg
      721 ctgccttagc gatcgtggtc ggccgcctgc catgagttgg cccgcgtgat ccgtcccggc
      781 gggcggctcg caatgacgac gtgggactac cactctcagc ccgtgaaccg cccgccccag
      841 gtccaagacc accggccgtt gctcgaggcg gccggcttcg aggtggaacg atacgacgac
      901 accgacgact gggaggcgcg gcaacgagcc acgacgcggg cgctactcga ccgagtcgag
      961 gacctcgccc tcgagtcggg ccagagcccg gacgacctcc gtgtcgggat agcggagatg
     1021 gctgcgacct tcgactgcat gatccgccgg gtgctcatcg tcgcccgccg cgcgtgatcc
     1081 ctccgcaccc gcgggttccg tgacgggtgt gctctcgacg tctgtctgtc tgctcagccg
     1141 acggcgaccg cagcttctcg ggcgtcatga cactacggag acacccgcgc aggaacgacc
     1201 gctgtgtcgc tgagggcgcg cgtgcaggtc atgcgagaag gggtatctcg aggtgggtgc
     1261 tgctccactg caccagatcg tccagccgtc ggcggggccc ttctcacgca ggacctgcgc
     1321 gtcttgctcg gcagcctgtc gacgcatctc tcgctccacc gccgccgcca cgagaacggc
     1381 tcggctagga gccttgccgg tggccaccgt gcggtccagg aaggccgcca tttcgtcggg
     1441 gagccgaaca gcgatcagag tgctcatgcc atccatcgtg atcgtcgcat cccacttcgg
     1501 atgcatgcgc acgcagcatt ccgcgcggtt cccgagagcc gttgagctgt cactaccaga
     1561 cctccagcac tgtcaccgat gtcctgatcc acgttcgtcc ccatgtcctg agacatgaca
     1621 gaggcacagc agcggtgtca tctcgcagga cataggtgac ggttctgcat caagacatcg
     1681 gtgacagtgt gggtcgcctt ggtggtgaca gttctgcccc gaggctcggg cggtgcctgc
     1741 tagtgaaccc atcgatcccc gtgtccgtct tgcgatctcg cggtggcctg aggacgcgcc
     1801 ccgcggggcg gtctcgacct tctgtgccga gcacgacatc tcccgcaagt cgttccacga
     1861 actacgcaaa cgcgccaaga ccgaggggcc ggcggccgtg ctcgagcccc ggacccgccg
     1921 accgaagacc agcccgtcga agctgaccga cgaggtcaag cagcaagcga tccaggtgcg
     1981 tgcggcgctg gaggcctccg ggctggacca cgggccgatc agcgtgcacg acaagatgca
     2041 cgcgatgggg ctgccggtgg tgccctcgac ggcgtcgctg gcgcggatct tccgtgaggc
     2101 cggtgtggcc cgcctcgagc cgaagaagaa gccccgttcg gcatggcgtc ggtttgtcta
     2161 cccggcgccg aacgcgtgct ggcaactcga tgccaccgag tacgtcctga ccggcggccg
     2221 caggtgtgtg atcttccagc tcatcgacga ccactcccgc tacgcggtgg cctcccacgc
     2281 cgcggccggc gagaccgcca aggacgccat cgcggtcttc gacaaggccg tggccgctca
     2341 cggtgtgccc caacgactgc tgtccgacaa cggacgcgcg ctcaacccct cccggctagg
     2401 gatcatcggc caactcctcg cacacgttgc cgcgttgggc accgaggcaa tcaccggcaa
     2461 gccgtacaag ccgaccaccc agggtaagaa cgaacgcttc caccagaccc tgttccgcta
     2521 cctcgacaag caacccctgg ccgagaccct cgaggagcta caggcccaga tcgacgcctt
     2581 cgaccacatc tacaacaccc agcgtcccca ccaagggctc cccggccgca ccacaccgct
     2641 ggccgcatgg gaggcgaccc ccaaggccga cccaccccgc ccgaagcccg accggcccgt
     2701 ctaccagccg ccggtcccgg catcgcgtcc ccgaccgaag ccgcccgcag acctacccgc
     2761 cgacacccgc atcagaacga tcagctcagt cggcaccatc cacgtcgaca aggtgttcta
     2821 caagatcgac gtacaccacg ccttccagca ggtcctggtc gtcagcgacg gcaaccagcc
     2881 cggcgacaag atcaccatca ccgacctcca aggcgagatc ctcgccgagc acaccagacc
     2941 cgcacccggc atccgctacg tagggaacgg ccgacccccc ggaccccgcc cgaagaaccc
     3001 agaagtgtca ccgaagccct gacacaccaa ccgtcaccga tgtcgtgatg cagaactgtc
     3061 acccatgtcc cgagacatca catcatcccc tggcaagtga gcagagcagc ggcgtgcgag
     3121 gaacgagcgc gtcgcgtggg ctcggccagg tcgagcgagc ccacggctga gcctgcgaag
     3181 ccgtgcaagg cgagtcgaga ccccgtgacg g
//
LOCUS       DATBFZ010000075         3522 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_5932378, whole genome shotgun sequence.
ACCESSION   DATBFZ010000075 DATBFZ010000000
VERSION     DATBFZ010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 3522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3522
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5932378"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(468..1799)
                     /locus_tag="VH419_02290"
     CDS             complement(468..1799)
                     /locus_tag="VH419_02290"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY7042476.1"
                     /translation="MRVAATFALITLLVTVALSAATYTLTRAYLLRNREDLATRQAFL
                     DGNVASGTLIDRASEPKDLVTSLGGDPGTQVLIHLDGEWYSSSVAIDPASLPSSLTAA
                     VASGNAARQRFSVAGVPHLAIGTPLAAAGAEYFEVVPLRELQRTLRTLLTYLVLASAV
                     TTAVGAAAGWWSSQRLLAPFRRISDVAVDVAKGSLDKRLDDDGDSDLSPLTTSFNEMV
                     NALQDRIAVEAQFAGDVSHELRTPLTALRTATAVVANDVDALPPRVRPAVDVLTNQVD
                     YFERLVLDLLEISRMDAGVDSLQLETVDVREVVVAAAKSAGGGPVEIVGSGPFEIDTD
                     KRRLGRVVVNLIENANRHGGGVTNVSVTRSDGHILVAVEDHGAGIASQDLDHVFDRFW
                     RAPNRDPGVRGTGLGLALVREHVKLMRGSIRLGPNEGAGTKAVIELPVDES"
     gene            complement(1829..2515)
                     /locus_tag="VH419_02295"
     CDS             complement(1829..2515)
                     /locus_tag="VH419_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020390558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY7042477.1"
                     /translation="MATRVMVVEDDADVRAMLLLLLRGEGYDVVEAATAEEAWERFSA
                     PKAEIDAVILDLKLPGRHGFELCRDLRQSSDVPIIIVTAQVHDADVVVGLESGADDYV
                     TKPFNPKVLTARLRALLRRTRSADAAGHLVFGPLVVKPREGRAERNGTPLPLTKTEFR
                     LLCDLAEHGDQLMSRDQLLERVWGYEFAGDGRLVDAHVRRIRMKIERDPAAPELLQTV
                     RGLGYRLVRP"
     gene            2740..3432
                     /locus_tag="VH419_02300"
     CDS             2740..3432
                     /locus_tag="VH419_02300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042478.1"
                     /translation="MELLTADPHPLRPRELVRPDVVVARCHPAVVDGARDDVCRLALE
                     LVEEGRRDSDEVELVADFLGPDAIASQRGLVSLGPDEWSAHRETFAEVMCLDRTSGDW
                     CRFERLNRRFATRAARVAPPEATLWVLLDGLVRSPLHLRRARPDITIIGLLLEPLPDP
                     IAVVRAASGAPLVDGILATDAVLLSALDEAARLEIAAVALDRADPCARGLVTADHAVV
                     LTTVFPGVARVR"
BASE COUNT          568 a         1198 c         1183 g          573 t
ORIGIN      
        1 gagctgcttg acctccggca cctcgctcag tgtgtatacg agctggccca ccgccaggag
       61 ctggtcgcca cggggcagct caccgaagtg gttcgagaga tcgatcgtgg ccgtcgggtc
      121 gctgaccgcg acgttactca ccacatcagt gctgatcgcg ctgcgaagtc cggccgacga
      181 ttccggggcc gatggtcccg tagcgagggc cgatatcagg ttcttgactg ctcctccagc
      241 caggtcccgt tcgacgggga cgagccgccc cgaacggatg aggtaaatcg tggtactcgt
      301 cgtgggcgac gccggtggtg attcggccgt ggtggttgtc ggcccgggga gaccgaacgg
      361 gacgtccttc cccgggatca cgtccgcgtg tccctggcct cgcacaccgc agcccacaca
      421 gatcatcagc aggccggcga ccatccatgc cgttcgtcga cgcctcatca agattcgtcc
      481 acggggagct cgatcacggc cttcgtacct gcgccctcgt tcggaccgag ccgaatcgag
      541 ccgcgcatca atttgacgtg ctcccgaacg agagcgagcc cgagcccggt gccacgaacg
      601 cccgggtccc ggttcggagc ccgccagaag cgatcgaaga catggtcgag atcctgcgaa
      661 gcgatcccag cgccatggtc ctcgacggct acgagtatgt gtccgtcgga tcgagtcacc
      721 gagacgttgg tgacgccgcc gccgtgccga ttggcgttct cgatcaggtt gaccacaacc
      781 cgtccgaggc gccgtttgtc cgtgtcgatc tcgaacggac ccgaccccac gatctcaacc
      841 ggcccgccgc cagccgactt ggcggccgct accacgacct cgcgcacatc gacggtttcg
      901 agctgcaagc tgtcgacgcc ggcatccatc cgtgagatct cgagcaggtc gagcacgaga
      961 cgctcgaagt agtcgacctg gttcgtcaac acgtcgactg ccggtcggac gcggggcggc
     1021 agcgcgtcga catcattggc cacgactgcg gtcgcggtcc gaagggcggt caagggagta
     1081 cgcagctcgt ggctgacatc accggcaaac tgcgcctcca ccgcgattcg gtcctgcagc
     1141 gcattgacca tctcgttgaa cgacgtggtc aggggcgaga ggtcactatc accatcgtcg
     1201 tcgaggcgct tgtcgagtga gcccttggcg acgtcgacgg ctacgtcgga gatgcgccgg
     1261 aacggcgcca ggagccgttg gctggaccac caccccgccg ccgcaccgac ggccgttgtg
     1321 accgccgatg ccagcaccag gtacgtcaac aaggttcgca gcgtgcgctg cagctcccgg
     1381 agcggaacga cctcgaagta ctccgctccc gcggcggcca gcggggtgcc gatggcgaga
     1441 tgcggtacgc ctgccaccga gaaacgctgg cgggcggcgt tgcctgatgc gaccgcggcc
     1501 gtgaggctgg acggcagcga tgccgggtcg atggcgaccg acgacgagta ccactcaccg
     1561 tccaaatgga tgagaacctg ggtgcccggg tcgccgccta gcgacgtgac gaggtccttg
     1621 ggctcgctgg cccggtcgat cagcgtgccg cttgcgacat tgccatcgag aaaagcttgg
     1681 cgcgtagcca ggtcctcccg gttacgaagg agataggcgc gggtgagcgt gtaggtcgcg
     1741 gccgaaagtg cgacggtcac gaggagcgtg atgagggcga acgtcgctgc gacccgcacg
     1801 cgaagaggaa gccgccgcct gaccttcttc acggccggac gagccggtag ccgaggccgc
     1861 gcacggtctg gagcagctcg ggggcggcgg ggtcacgctc gatcttcatt cggatgcgcc
     1921 ggacgtgggc gtcgacgagc cggccgtcgc cggcgaactc atagccccag actcgctcga
     1981 gcagctggtc ccgagacatg agttggtctc cgtgctcggc aagatcgcac agcaggcgga
     2041 actccgtctt cgtcagcggc aacggagttc cgttgcgctc ggctcggccc tctctcggct
     2101 tgaccacgag agggccgaag acaagatggc cggcggcgtc cgcgctccgt gtccgacgga
     2161 ggagcgctcg gagccgggcc gtgagcacct tggggttgaa cggctttgtc acataatcgt
     2221 cggcgccgga ctccaggccg acgacgacgt ccgcgtcgtg aacctgggcg gtgacgatga
     2281 tgatgggcac gtcgctcgac tggcgcaggt cccgacacag ctcgaagccg tgccggccgg
     2341 gcagcttcag gtcgaggatc accgcgtcga tctcggcctt cggcgcggag aaccgttccc
     2401 atgcttcctc cgctgtggcc gcctcgacga cgtcgtagcc ctcgccccgc agaaggagca
     2461 ggagcatcgc acggacgtca gcgtcgtcct ccacgaccat cacccgggtg gccactcacc
     2521 gatcatcgtc cccgggggcg cccgcttcca ttcattggcc tggccgttcc tgaacgagcg
     2581 cgaaacgaca acacgctcgc tacccgatga caatgcgacg ccggggggct ttcgccgaac
     2641 ggcaaccgag catcgcggtg cacgcaacat gcgcttgcca ccgtaggggc cgtgtggtgc
     2701 cggccaagga gccggccgga ccggcgaggg aggatctctg tggagctgtt gacggccgat
     2761 ccgcaccccc tccggccgcg cgagctcgtc cgcccggacg tcgtcgtggc ccgttgccat
     2821 ccggctgtcg tcgacggtgc ccgcgacgac gtctgccgtc tcgccctcga gctcgtcgag
     2881 gaaggacgac gtgactcgga cgaggtcgaa ctcgtcgccg acttcctcgg cccggacgcc
     2941 atcgccagcc agaggggtct tgtctcgctc gggccggatg aatggtccgc ccaccgggag
     3001 acgtttgcgg aagtgatgtg cctcgaccgg accagcgggg actggtgccg gttcgagcga
     3061 ttgaaccgac ggtttgcgac gcgggcggct cgggtcgcgc cgcccgaggc gacgttgtgg
     3121 gtccttctcg acgggctcgt ccgcagcccc cttcaccttc gccgagcgcg acccgacatc
     3181 acgatcatcg ggctgctgct cgagccactg cccgacccga tcgccgtcgt gcgcgctgca
     3241 tcgggtgctc cgctcgtgga cggcatcctc gccaccgacg ccgttcttct ctcggccctc
     3301 gatgaagcgg cgcgcctcga gatcgcggcg gtggcgctcg accgtgccga cccctgtgcc
     3361 cgaggcctgg tgaccgccga tcacgccgtg gtgctcacca ctgtcttccc tggggttgcc
     3421 cgagtcaggt gacgttcggg cgcaggagat gcgtcccgcg gaacgttgtc tacaacttgt
     3481 ctaccgccgg gcaagaaatc gcgcgtcgcc gacctacgta gc
//
LOCUS       DATBFZ010000076         2389 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_3331466, whole genome shotgun sequence.
ACCESSION   DATBFZ010000076 DATBFZ010000000
VERSION     DATBFZ010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3331466"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1068
                     /locus_tag="VH419_02305"
     CDS             <1..1068
                     /locus_tag="VH419_02305"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004811652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HEY7042479.1"
                     /translation="KNDIADPDSVDINESVPCPHCGNRHTWTEPRNFNMMLKTYLGVI
                     EDDSGLHYLRPETAQGIFLNFANVVTSSRMKPPFGIAQMGKSFRNEITPGNFIFRTRE
                     FEQLEMEFFVKPGEDEHWHQYWIDERTRWYTDLGIDRDHLRHYEHPQEKLSHYAKRTV
                     DIEYDFGFSERGFEELEGVANRTDFDLSQHSEFSGQDLSYFDQASGERFLPYVIEPAG
                     GLTRSLMAFLLDAYSEDEAPSAKGGVDKRVVLRLDPRLSPVKVAVLPLSRNADLSPKA
                     RDLAAQLRGHGWNVEFDDSGAIGRRYRRQDEIGTPYCVTVDFETLDDHAVTVRWRDSM
                     KQERISLDQTSAFFAEHLLGC"
     gene            1070..1744
                     /locus_tag="VH419_02310"
     CDS             1070..1744
                     /locus_tag="VH419_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042480.1"
                     /translation="MTRSYALPALAAVAALVLAGCGGGGSSEPAAGGPDTSSTPTSTP
                     TSSSTSPGSSSTGSDVVDGVTLTPEGSRLRVGDTARVSWQPNQKTTGVVAMTVTGLLK
                     MPISAFSAWRLNRETRRSTPYFVHATVRNLGKSDLSHVPVPLYLLDQRQTLLQASTFQ
                     ATFTPCPSRPLPAKFRHGDKTSVCLVYFVPHHGKLVAVSFRPTQDFDAITWQGDVVNR
                     GHHKKH"
     gene            1783..>2389
                     /locus_tag="VH419_02315"
     CDS             1783..>2389
                     /locus_tag="VH419_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HEY7042481.1"
                     /translation="MPLTLGPLTVPVPVVLAPMAGITNAAYRRLCAEQGAGLYVCEMI
                     TSRGLVERDSTTLKMLVFDDLEKTRSVQLYGTDPSYVGKATEILCAEYGVDHVDLNFG
                     CPVPKVTRKGGGGALPWKRGLLAEILTAAVAAAEPYGVPVTMKTRKGLDDDHLTYLDA
                     GRIAEDSGVVAIALHGRTVAQSYSGQADWGTIGRLVEHVSIP"
BASE COUNT          393 a          876 c          767 g          353 t
ORIGIN      
        1 aagaacgaca tcgccgaccc cgacagcgtg gacatcaacg agtccgtgcc ctgcccccac
       61 tgcggcaacc gacacacctg gaccgagccg cgcaacttca acatgatgct gaagacctac
      121 ctcggcgtca tcgaggacga ctcgggtctg cactacctgc gtcccgagac cgcccagggc
      181 atcttcctca acttcgccaa cgtggtgacc agctcgcgga tgaagccgcc gttcggcatc
      241 gcccagatgg gcaagagctt ccgcaacgag atcacgccgg gcaacttcat cttccgcacc
      301 cgcgagttcg agcagttgga gatggagttc ttcgtcaagc cgggcgagga cgagcactgg
      361 caccagtact ggatcgacga gcgcacccgc tggtacaccg atctcggcat cgaccgtgac
      421 cacctgcgcc actacgagca cccgcaggag aagctcagcc actacgccaa gcgcaccgtg
      481 gacatcgagt acgacttcgg cttctccgag cgtggcttcg aggagctgga gggtgtcgcc
      541 aaccgcaccg acttcgacct ctcccagcac agcgagttct ccgggcagga cctctcctac
      601 ttcgaccagg ccagcggtga gcggttcctc ccctacgtga tcgagccggc gggcgggctg
      661 acccgcagcc tgatggcgtt cctgctcgac gcctactccg aggacgaggc ccccagcgcc
      721 aagggcggtg tcgacaagcg cgtcgtgctc cgtctcgacc cacgcctgtc gccggtcaag
      781 gtcgccgtgc tcccgctcag ccggaacgcc gacctgtcgc cgaaggcgcg tgacctcgcc
      841 gcccagctcc gcgggcacgg ctggaacgtc gagttcgacg actccggcgc gatcggccgt
      901 cgttaccggc gacaggacga gatcgggacg ccgtactgcg tcaccgtcga cttcgagacg
      961 ctcgacgacc acgccgtgac ggtgcggtgg cgtgactcga tgaagcagga gcggatctcc
     1021 ctcgaccaga cgagcgcctt cttcgcggag cacctgctcg ggtgctgacg tgacccgctc
     1081 ctacgccctg cccgccctcg cggccgtcgc cgctctcgtg ctcgccggct gcggaggcgg
     1141 tggctcgtcg gagccggccg ccggcggccc ggacacctcg tcgacgccca cgtcgacgcc
     1201 cacgtcctcg agcacgagcc ccggctcgag ctccaccggt tccgacgtcg tcgacggggt
     1261 gacgctcacg cccgagggct cacggctccg ggtcggcgac accgcccggg tcagctggca
     1321 gccgaaccag aagacgacgg gcgtcgtcgc gatgaccgtg accggcctgc tgaagatgcc
     1381 gatcagcgcc ttcagcgcgt ggcggctcaa ccgcgagacc cggcgctcca cgccgtactt
     1441 cgtgcacgcc acggtccgca acctcggcaa gagcgatctc tcgcacgtgc cggtgccgct
     1501 ctacctgctg gaccagcggc agacgctgct gcaggcctcg accttccagg cgacgttcac
     1561 cccgtgcccg agccggccgc tgccggcgaa gttccggcac ggcgacaaga cctcggtttg
     1621 cctggtctac ttcgtgcccc accacggcaa gctggtcgcg gtcagcttcc gtccgacgca
     1681 ggacttcgac gcgatcacct ggcagggcga cgtcgtcaac cgcggacacc acaagaagca
     1741 ctgagtttgg tcagcacccg gctcgccccg gacaatcggg ccatgcctct gaccctcggc
     1801 ccgctgaccg tgcccgtgcc ggtcgtgctc gcgccgatgg cgggcatcac caacgcggcg
     1861 taccggcgtc tgtgcgcgga gcagggcgcc ggcctgtacg tctgcgagat gatcaccagc
     1921 cgcgggctgg tcgagcgcga ctccacgacc ctgaagatgc tggtcttcga cgacctcgag
     1981 aagacccgct cggtgcagct gtacggcacc gacccgtcgt acgtcgggaa ggcgaccgag
     2041 atcctctgcg cggagtacgg cgtcgaccac gtcgacctga acttcgggtg tccggtcccc
     2101 aaggtgaccc gcaagggtgg cggcggtgcg ctgccctgga agcgcggcct gctcgccgag
     2161 atcctcaccg ccgcggtcgc cgcagccgag ccgtacggcg tgccggtgac gatgaagacg
     2221 cgtaaggggc tcgacgacga ccacctgacc tacctcgacg ccggtcgcat cgcggaggac
     2281 tccggcgttg tcgcgatcgc cctgcacggc cgaacggtcg cgcagtccta cagcggacag
     2341 gcggactggg ggacgatcgg ccggttggtc gagcacgtgt cgatcccgg
//
LOCUS       DATBFZ010000077         2664 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_1689381, whole genome shotgun sequence.
ACCESSION   DATBFZ010000077 DATBFZ010000000
VERSION     DATBFZ010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1689381"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1283)
                     /gene="ettA"
                     /locus_tag="VH419_02320"
     CDS             complement(<1..1283)
                     /gene="ettA"
                     /locus_tag="VH419_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002516980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HEY7042482.1"
                     /translation="MADYVFTLRNVRKALGDKVVLDNVTLSFLHGAKIGVVGPNGTGK
                     STLLKIMAGLEQPSNGDAIRDPDATVGMLQQEPPLTEGTTVLENVEEAVHEIKGKVDR
                     YNAISAELADPDADYDTLLAEMGDLQTDLDHAGAWDLDSRLDQAMDALRCPPPDWPVE
                     TLSGGERRRVALCKLLLQQPDLLLLDEPTNHLDAESVQWLEGHLASYPGCVLAVTHDR
                     YFLDNVAEWILELDRGKTHPYEGNYSTYLETKKQRLQIEGQKDAKRSRMLERELEWVR
                     SNPKARQAKSKSRLARYEEMAAEAVRNRKVDLSEINIPPGPRLGDLVLEADGLGKSFG
                     DRVLMHDLDFKLPRAGIVGVIGPNGVGKTTLFRMIIGEEKPTEGELRVGQTVKISYVD
                     QSRAGIEPNKNVWEVVSDGLDHIKVASFEMPSRAYV"
     gene            complement(1362..1844)
                     /gene="ssb"
                     /locus_tag="VH419_02325"
     CDS             complement(1362..1844)
                     /gene="ssb"
                     /locus_tag="VH419_02325"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEY7042483.1"
                     /translation="MTETILHVVGHVGTDIDHRRVGDGVDLSTFRLATTPRRWDRQQR
                     AYVDGTTTWFTVQCWRQLAANVRDSLRRGDPVVVIGRLKTEEWTKDDVRNSRIVLEAF
                     SVGHDMARGVSSFRKTQKPVEAADEGANEVREALEDIERVEPTAYDENDPRGNLVVAS
                     "
     gene            complement(1995..>2664)
                     /locus_tag="VH419_02330"
     CDS             complement(1995..>2664)
                     /locus_tag="VH419_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEY7042484.1"
                     /translation="RTATGWPVTKWLSGLRPDPLRRLHLDGVQRTGRTSLPEPTPVQR
                     ARVDGAVRSVADAVTAGMARPWASAIRDASLSRLDGFSQALDAAVASTELGARREPLW
                     WRGVRALQWLLFLVAVAGGLWLLTLAVFAFLRLPEPDSVDWHGLPVPTLMLLGGVAVG
                     VLVAVGCRYAARVSARRRAARARTRLRSAIASVTDELVVDPMGREIDAYNRCRDGVRM
                     ALRA"
BASE COUNT          408 a          930 c          874 g          452 t
ORIGIN      
        1 acgtatgcac ggctcggcat ctcgaagctg gcgaccttga tgtggtcgag cccgtcggac
       61 acgacctccc acacgttctt gttcggttcg attcccgccc ggctctggtc gacgtacgag
      121 atcttgacgg tctgaccgac cctgagctcg ccctcggtcg gcttctcctc gccgatgatc
      181 atccggaaca gcgtggtctt cccgacgccg ttgggtccga tcacgccgac gatgccggcg
      241 cgcggcagct tgaagtcgag gtcgtgcatg aggacccgat ccccgaacga cttgccgaga
      301 ccgtccgcct cgagcacgag gtcgcccagc cgtgggccgg gcgggatgtt gatctcgctc
      361 aggtcgacct tgcggttccg aacggcctcc gcggccatct cctcgtatcg cgccaaccgt
      421 gacttgctct tggcctgccg cgccttcgga ttcgatctca cccattcgag ctcgcgctcg
      481 agcatcctgg accgtttggc gtccttctgc ccctcgatct gcaggcgctg cttcttcgtc
      541 tcgaggtatg tcgagtagtt cccttcgtac gggtgggtct tcccgcggtc gagctcgagg
      601 atccactcgg ccacgttgtc caggaagtac cggtcgtgcg tgacggcgag cacacacccc
      661 gggtacgacg ccaggtgacc ttccagccac tgcaccgact cggcgtcgag gtggttggtc
      721 ggctcgtcca ggagcagcag gtcaggctgc tggaggagca gcttgcacag cgcgacccgg
      781 cgacgctcac caccgctcaa cgtttcaacc ggccagtccg gcggcggaca ccgcagcgcg
      841 tccatcgcct ggtccagccg actgtccagg tcccatgcac cggcgtggtc gaggtcggtc
      901 tgcaggtcgc ccatctcggc gagcagcgtg tcgtagtcgg cgtccggatc cgccagctcg
      961 gcagagatcg cgttgtagcg gtcgaccttc cccttgatct cgtgaacggc ctcctcgacg
     1021 ttctcgagca cggtcgtgcc ttcggtcagc ggaggctcct gctggagcat gcccacagtg
     1081 gcgtcgggat cacgaatggc gtcaccgttc gacggctgct cgagaccggc catgatcttc
     1141 agcagcgtcg acttcccggt gccgttgggc ccgacgaccc cgatcttggc gccgtgcaag
     1201 aaggagaggg tcacgttgtc gaggacaacc ttgtcgccga gggctttccg gacattgcgc
     1261 agggtgaaga cgtagtcggc catgatgccg aagcctacga cgggtcttgc ggcatctccg
     1321 aatccacccg tgctctgttg gcgcagcacc agcaaggcgg gtcatgacgc caccacgagg
     1381 ttcccacgag ggtcgttctc gtcgtacgcc gtcggctcga cacgctcgat gtcctcgagc
     1441 gcctcacgga cctcgttggc accctcgtcg gccgcttcaa ccggcttctg cgtcttgcgg
     1501 aacgagctga ctccgcgagc catgtcatgc ccgacggaga acgcctcgag cacgatgcgg
     1561 gagttgcgga cgtcgtcctt cgtccactcc tcggtcttga gtcgcccgat gacgaccacc
     1621 ggatcgccgc ggcggagcga gtcgcgcacg ttggcggcga gctggcgcca gcactggacg
     1681 gtgaaccagg tcgttgtgcc gtccacgtag gcgcgctgct ggcggtccca tcggcgcggc
     1741 gtggtggcca gtctgaacgt gctcagatcg acgccgtcac cgacgcggcg gtgatcgatg
     1801 tcggtgccga cgtgtccgac gacgtgcagg atggtctcgg tcatggctgc tcctcctcgc
     1861 tcgttgctca gcggctgcag cctcgtcgct gcacgcgacg gtgggcgtgc ggcgagcgtg
     1921 accctgtgga cagccgcccg cacgaacccg ggctggggaa acggtggtgg cttggcgctg
     1981 tggcgagccg ggcgtcaggc gcgaagggcc atgcgtaccc cgtcgcgaca tcggttgtag
     2041 gcgtcgatct cgcgccccat cggatcgacg acgagctcat cggtgaccga tgcgatcgcc
     2101 gaccggagcc gggtccgtgc cctcgcggcg cgtcgtcgcg cggacacccg ggcggcgtac
     2161 cggcagccga cggccaccag cacgccgacg gcgaccccgc cgagcagcat caacgtcgga
     2221 accggaagac cgtgccaatc gacgctgtcc ggttcgggga gtcgcaggaa ggcgaaaacc
     2281 gccaacgtca acagccacaa acccccggca acggcgacca ggaacaacag ccactgcagc
     2341 gcacggacgc cgcgccacca aaggggttcg cgccgggcac cgagttcggt gctcgcgacc
     2401 gctgcgtcca gcgcctgcga gaaaccgtcc agccgcgaca gcgacgcatc ccggatcgcg
     2461 gacgcccagg gcctcgccat ccccgctgtc accgcatcgg cgacggagcg aaccgcaccg
     2521 tcgacacgag cccgctggac cggtgtgggt tcaggcaggg aggttcgccc ggtcctctgc
     2581 acgccgtcca ggtgaagccg ccgcagcggg tccggccgaa gccccgacag ccacttcgtc
     2641 accggccacc cagttgcggt ccgg
//
LOCUS       DATBFZ010000078         5739 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_1363243, whole genome shotgun sequence.
ACCESSION   DATBFZ010000078 DATBFZ010000000
VERSION     DATBFZ010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 5739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5739
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1363243"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1097
                     /locus_tag="VH419_02335"
     CDS             <1..1097
                     /locus_tag="VH419_02335"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HEY7042485.1"
                     /translation="HAGLPAEATGARPGPISVFLKVASGVLSGALIVLLFMAIIPKVT
                     EYQSVGDSLKSMSPLTIALLFVMAVLIRLVLAASYDAVMPGLSLWRSLVAREASSAVS
                     NVIPGPSGTAAQWAILRSWDVTTERFAQATLAVSVSTDVLILAGPGAFFVLWALSGMP
                     DSPDGGNAWAFGLGAVILSVLSVVVVAGVAKSMKLAGRLGRLCQACVNPLRRVISKPR
                     ITDLPEKFVVLRADMIEVLRLYGRMLLSWVVSGYLLNGVLLVACLWACGASRNELPMS
                     LGLMLYAVGRIATIIQITPGGVGVVEIAYTSVYVAVLGESAQPAIVAGVLVYRMFTYL
                     LPIITGAFAYVVWRVMRRSELHHEAAAESA"
     gene            1176..2165
                     /locus_tag="VH419_02340"
     CDS             1176..2165
                     /locus_tag="VH419_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008363440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF389 domain-containing protein"
                     /protein_id="HEY7042486.1"
                     /translation="MLQLRISAPTSLSDDVLRILSDDPAVTGLAVVNAASLRPAGDVV
                     FAEVAREAANDIIDRLLSIGLQREGSLHVQPVDTWVSQRAFDAEREAPGSGADAVVWA
                     EVTQRAYAESELNWTYLSFMCLATLIAAIAIILDSQILVIGAMVLGPEFGAVAGLGLA
                     LVRRRKVLLVRTIRALLLGFAVAICVTALTALVGRWLGWLAAADVTGPRPGTDFIYTP
                     DRWSFVVSVIAGAAGVLSLTSSRVGGLSGVFISVTTIPAAGNVGLALAFGLSHEARGS
                     TLQLLINLSGMAVAGFVTLILQQLVWARVSARRQRLSTRLRRRDRDGPARPSD"
     gene            complement(2562..3836)
                     /gene="lysA"
                     /locus_tag="VH419_02345"
     CDS             complement(2562..3836)
                     /gene="lysA"
                     /locus_tag="VH419_02345"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEY7042487.1"
                     /translation="MTDVARGELLALFPPDSSTDETGMLLLGGCRADTLAAEYGTPVL
                     VVSEHTIRTRAREYADGLVSRWPNARVLFASKAFPCTSVQRLMVEEGLGLDVAGGGEV
                     RSALKAGARSADLVLHGNAKTDEDIRQAVHLRMGLVVVDNADDVDRLEAIVPTGDRQD
                     VLVRVIPGIKADTHDHVLTGHEESKFGLPLEAATRLIQRIESSSRMRMRGLHVHVGSQ
                     IMDLDPFVQSVERLRQLGEFPVYDLGGGLGARYTYADQPPSIAQYLDALVSAAHACLP
                     ADARLLIEPGRSIVASAAATLYHVVTVKRAAKTFVAVDGGMADNLEVSLFGQRFEAGI
                     VDRLEGARGETVTVVGRHCESGDVLVDKVTLSRPKVGDLLAVPATGAYSFTMSNNYNG
                     ARRIPVVFVRDGRARLVVRRETWDDLLARDVD"
     gene            complement(3833..4756)
                     /locus_tag="VH419_02350"
     CDS             complement(3833..4756)
                     /locus_tag="VH419_02350"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY7042488.1"
                     /translation="MTMVVGYAPDERGRTALHLTSLLTRSTDDEIVVCSVVPSPWLPG
                     MARVDAEYRATLEKEADAALEHAKAAMPSDVNATYVRHSARSAPAGLLEVARNREARL
                     IILGSSSAGVFGHVVLGSTTERLLHGSPVAVALAPRGFRCGPDTTITRVTAAYDDSEN
                     ANTLVVAASTLAAEVGAGLRIASFAVWSQPAYTTRLGTDSEDDVLQSWTSSIEQAAAL
                     ALGRVADLASAPPKLETVIGRGPDWGAAIDEVEWHDGDVLIVGSSSIGPLAQVFLGTR
                     ASKIVRYAPVPVMVLPRRRAELLASEANVRA"
     gene            complement(4753..>5739)
                     /locus_tag="VH419_02355"
     CDS             complement(4753..>5739)
                     /locus_tag="VH419_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEY7042489.1"
                     /translation="APEQGGIINIPAVILVVLCAMLLIRGASESAKVNAVMVMLKIGV
                     LIMFVVIGLQGWNSDNLTPFAPDGLHGVTSAAGIIFFSYIGLDAVSTAGEEVKNPRRN
                     MPLAILIALVTVTSLYVLVTTVAVAAQPASAFEGQEAGLAKILENVTGETWPATVIAA
                     GAVISIFSVTLIVLYGQTRILFAMSRDGMIPQLFHKVNPRTLTPVPNTIIVATIIAVM
                     AAFIPIDFLAEMTSIGTLVAFLVVSIGVMVLRWTHPELPRGFKVPLFPVTPILSIAGC
                     IWIIQDLRPVTIYVFVIWVTVALLWYFAYGIKHSHLGRHEHVGLTDDEAPSP"
BASE COUNT          922 a         1951 c         1880 g          986 t
ORIGIN      
        1 ggcacgccgg gctgcccgca gaggcaaccg gcgcacggcc aggcccgatc tcggtcttcc
       61 tcaaggtggc cagcggcgtg ctgtccggcg ccctcatcgt cttgctgttc atggcgatca
      121 tccccaaggt caccgagtac cagagcgtcg gcgactccct gaagtcgatg tcgcccctga
      181 cgatcgccct gctgttcgtg atggcggtgc tgatccggct cgtcctcgcc gcgtcgtacg
      241 acgccgtcat gcctggcctc tcgctgtggc ggtcgctcgt cgcgcgagag gcgtcctcgg
      301 cggtcagcaa cgtcatcccg ggcccttcgg gcaccgcggc acaatgggcg atcctgcgct
      361 cgtgggatgt caccaccgaa cggttcgccc aggcgaccct ggcggtgagc gtcagcaccg
      421 acgtcctcat cctcgccggc cccggcgctt tcttcgtgct gtgggcactg tccgggatgc
      481 ccgactcgcc cgacggcggg aatgcctggg cgtttggtct cggtgccgtc atcctctcgg
      541 tgctttccgt cgtcgtcgtc gccggcgtcg cgaagagcat gaagctcgcc gggcgactgg
      601 gccggctctg ccaggcctgt gtcaacccgc tccgcagggt catcagcaag ccccgtatca
      661 cagacttgcc agagaagttc gtggttcttc gtgcggacat gatcgaggta ctccgcctct
      721 acggccgaat gctgctcagc tgggtcgtgt ccggctactt gctgaacggc gttctgctgg
      781 tcgcgtgcct gtgggcctgc ggcgcctctc gcaacgagct gcccatgtca ctggggctga
      841 tgctgtatgc cgtcgggcgc attgcgacga tcatccagat cacgccgggc ggtgtcggtg
      901 tcgtcgagat cgcctacacc tcggtgtacg tcgccgtgct cggcgagtcg gcccagcctg
      961 cgatcgtcgc gggcgtgctc gtctaccgga tgttcaccta tctgctgccg atcatcaccg
     1021 gcgccttcgc gtacgtcgtc tggcgggtca tgcgccgctc cgagctgcat cacgaggcag
     1081 cggccgagtc cgcctgagac gcacgcctcg caaaccctgt acgaccccac ctcagcgcgc
     1141 tgaagcgcaa gcgcagactg cgcgagactt tccgcatgct gcagctcaga atctctgccc
     1201 cgacatcgtt gagcgacgac gtcctacgga ttctgtccga cgacccggcc gtgacgggcc
     1261 tcgcggtggt gaacgccgcc tccctcagac ccgcagggga cgtggtgttc gccgaggtgg
     1321 cgcgagaggc ggccaacgac atcatcgacc gtctcctgtc catcggactt caacgcgagg
     1381 gaagtctgca tgtgcaaccc gtcgacacct gggtctccca acgcgcattc gacgccgaac
     1441 gggaggcgcc cggcagcggc gcagacgccg tggtgtgggc cgaggtcacg cagcgcgcct
     1501 atgcggagtc agagctgaac tggacctatc tcagcttcat gtgtctcgcg acgctgatcg
     1561 cggccattgc gatcatcctg gactcgcaga ttctggtcat cggtgcgatg gtgctcggcc
     1621 cagagttcgg tgcggtggcc ggcctcgggc tggctcttgt tcgtcggcgc aaggtcttgc
     1681 tggtccggac gatccgggca ctgctgctcg gattcgcggt cgcgatctgc gtcaccgcgc
     1741 tcaccgctct ggtgggtcgt tggctcggat ggctggccgc ggctgacgtc acgggtccgc
     1801 ggcctggaac tgacttcatc tacacgcccg atcggtggtc cttcgtcgtc tcggtcatcg
     1861 ccggggcggc cggtgtgctc tccttgacat cgtcacgagt cggcggactt tccggtgtgt
     1921 tcatctccgt gaccaccatc ccggctgcgg gcaacgtcgg actcgcgctt gcgttcgggc
     1981 tcagccatga ggcacgggga agcacgctcc agctgctgat caacctctca ggtatggccg
     2041 tcgccggctt cgtcacgttg atcctgcagc aactcgtctg ggcgcgtgtc tcggcaaggc
     2101 gacagcgact cagcacccgg ttgcgtcgga gggaccggga tggtcccgcc cgtccctccg
     2161 actgaggtgc cggagctgcc ggctcgtggt gatgatcgac gccacctggt cggccgggca
     2221 gatctcggct ctcctcacgt atctccgggc cgcgtcggtg gtggtttgcc tgggtagcca
     2281 tccaaacgga cgggtagcca ggcaggtctg ccttgggagg gatgcgtatg gctggttagc
     2341 catgcaaacc gcggctcggc accacggatg tccgaaaccc gccagtcaca cgccaggcaa
     2401 aagggacgta aaagccggcc ggccacctca gcaggaactt cccccgctct tcaaagccga
     2461 tccacctcca cccgaggacg accgaacgcc gggagaccac ggccaccgtc gcgaccatct
     2521 gcgcgctaca gggacctgag gacttgtagg gcaggaagca ctcagtcgac gtcgcgtgcg
     2581 agcaggtcgt cccacgtctc ccgccgtacg acgagacgcg ccctgccgtc ccggacgaac
     2641 acgaccggga tgcgccgggc tccgttgtag ttgttcgaca tcgtgaagct gtaggcaccg
     2701 gtggccggca cggcgagcag gtcaccgacc ttcggcctcg acaacgtcac cttgtcgacc
     2761 agcacgtcac cgctctcgca atgccggccg acgacggtca cggtctcacc gcgggcgcct
     2821 tccaaccggt cgacgatccc ggcttcgaac cgttggccga acagggacac ctcgaggttg
     2881 tccgccatcc cgccgtcgac cgccacgaat gtcttggcgg cacgcttcac cgtgaccacg
     2941 tggtacagcg tcgcagcggc cgaggcgacg atgctccggc caggctcgat cagcagtcgc
     3001 gcatccgccg gaagacaggc atgcgcggcg gaaacgagcg cgtcgagata ctgcgcgatc
     3061 gagggtggtt ggtcggcgta cgtgtacctg gcaccgagac cgccgccgag gtcgtaaacc
     3121 gggaactcgc cgagctgccg cagccgctcg acggactgga cgaacggatc gaggtccatg
     3181 atctgtgagc cgacatggac gtggaggcca cgcatgcgca tcctcgagct gctctcgatc
     3241 cgttggatca gccgcgtggc tgcctcgagc gggaggccga acttcgactc ctcatgaccg
     3301 gtgagcacat ggtcgtgcgt gtccgccttg atgccgggaa tgacgcggac cagaacgtcc
     3361 tgcctgtctc ccgtcggaac gatcgcttcg agcctgtcca cgtcgtcggc gttgtcgacg
     3421 acgacgagcc ccatgcggag gtggaccgcc tgacggatgt cctcgtcggt cttggcgttg
     3481 ccatgcagaa cgaggtcggc ggagcgagct cctgctttca gcgcggagcg gacttcacca
     3541 ccacccgcga cgtcgaggcc caagccctct tcgaccatca accgttgcac ggatgtgcag
     3601 gggaacgcct tcgacgcaaa cagcacgcga gcgttcggcc aacgggagac gaggccgtcc
     3661 gcgtactcgc gggccctggt ccggatcgtg tgctctgaca cgacgaggac cggcgtaccg
     3721 tactccgcgg ccaacgtgtc agcccggcaa ccgcccagca gcagcatgcc ggtctcgtcg
     3781 gtcgagctgt ccggcgggaa cagcgcgagc agctctcctc gggcgacgtc cgtcatgctc
     3841 ggacgttcgc ctcgctggcc agcagctccg cgcgtcggcg cgggaggacc atgaccggga
     3901 ccggcgcgta ccggacgatc ttgctggcac gcgtcccgag gaacacctgc gcgagcggac
     3961 cgatgctgct ggagccgacg atcagtacgt cgccgtcgtg ccattcgact tcgtcgatgg
     4021 cggcgcccca gtccggcccc cgaccgatca cggtctccag cttgggtggg gccgacgcga
     4081 ggtcagcgac gcgtccgagt gcgagcgcag cggcctgctc gatcgacgac gtccaactct
     4141 ggagcacatc atcctcggag tccgtaccga gacgggttgt gtacgccggc tgcgaccaca
     4201 cggcaaatga cgcgatgcgc aacccggcgc cgacctccgc ggcaagggtg ctggccgcca
     4261 cgaccaaggt gttcgcgttc tcgctgtcgt cgtacgccgc cgtgacgcgg gtgatcgtgg
     4321 tgtcgggtcc acatcggaaa cctcgaggcg ccagggcaac cgcgaccggc gaaccgtgca
     4381 gcagccgctc cgtcgtgctg ccgagtacga cgtggccgaa cacgcctgcc gacgacgacc
     4441 cgaggatgat gagccgggct tcgcggttcc gcgccacctc gagcagaccg gcaggcgcgg
     4501 atcgtgcgct gtgccggacg tacgtcgcat tcacgtcaga cggcatcgct gccttcgcgt
     4561 gctcgagcgc ggcgtccgcc tccttctcca gcgtcgcgcg gtactcggcg tcgactcggg
     4621 ccatccccgg gagccacgga ctcgggacga ccgagcacac cacgatctcg tcgtcggtcg
     4681 agcgcgtcag caggctcgtc agatggagcg ccgtgcgacc tcgttcgtcc ggggcgtacc
     4741 cgaccaccat cgtcatggtg acggtgcctc atcatcggtc aggcccacgt gctcgtgccg
     4801 gccgaggtgg gagtgcttga tgccgtaggc gaagtaccac agcaaggcca cggtgaccca
     4861 gatgacgaac acgtagatcg tgacgggccg caggtcctgg atgatccaga tgcagccggc
     4921 gatcgacagg atcggagtga ccgggaacaa cggcaccttg aagccgcgtg ggagctccgg
     4981 gtgggtccac cgcagcacca tcacaccgat cgagaccacg aggaacgcga ccagcgttcc
     5041 gatgctcgtc atctccgcaa ggaagtcgat ggggatgaac gcggccatca cggcgatgat
     5101 cgtggccacg atgatcgtgt tggggaccgg ggtcaacgtg cgcgggttca ccttgtggaa
     5161 cagctgcggg atcatgccat cccgagacat cgcgaacagg atccgcgtct gtccgtagag
     5221 cacgatcagg gtcacgctga agatcgagat caccgcgccc gcagcgatca ccgtcgccgg
     5281 ccatgtctcc cccgtcacgt tctccaggat cttggccagg ccggcctcct ggccttcgaa
     5341 cgccgaggcc ggctgcgccg ccaccgccac cgtcgtcacg aggacgtaga ggctggtcac
     5401 agtgaccaac gcgatgagga tcgccaacgg catgttccgc cgcgggttct tgacctcctc
     5461 cccggcggtc gagactgcgt ccagaccgat gtaggagaag aagatgatgc ctgctgccga
     5521 cgtcacgccg tgcaggccat caggtgcgaa cggcgtgagg ttgtcggagt tccatccctg
     5581 gagcccgatc accacgaaca tgatcaggac accgatcttg agcatgacca tgaccgcgtt
     5641 gaccttggcc gactcacttg ctccgcggat cagcagcatc gcgcacaaca cgacgaggat
     5701 cacggcgggg atgttgatga tgccgccctg ctccggggc
//
LOCUS       DATBFZ010000079         2018 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_3635528, whole genome shotgun sequence.
ACCESSION   DATBFZ010000079 DATBFZ010000000
VERSION     DATBFZ010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3635528"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="VH419_02360"
     CDS             <1..272
                     /locus_tag="VH419_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017933283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GPR1/FUN34/YaaH family transporter"
                     /protein_id="HEY7042490.1"
                     /translation="AWAIFTAYMCVAAARVSTAVLSVFVLLTITYLALTIGWFDQESS
                     GWIKLGGYLGILTAIAAWYTSFAGVTAFTWGRQVFPVGPRNAPRD"
     gene            395..>2018
                     /gene="acs"
                     /locus_tag="VH419_02365"
     CDS             395..>2018
                     /gene="acs"
                     /locus_tag="VH419_02365"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020664180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HEY7042491.1"
                     /translation="MSEETLSNLLHEERRFEPPADLAAKANVTVDAYDEAESDFVAFW
                     EGAAERLTWDTRWTRGLDWDDAPFAKWYVGGKLNAAYNCVDRHVENGHGDRVAYHWVG
                     EPEDDTRDITYAELKDMVCQAANALVDLGVKTGDRVAIYMPMIPETVVAMLACARLGA
                     PHTVVFGGFSSTALRDRVQDCDARIVITADGGYRRGAAAALKPAVDEALQQCPDVRNV
                     LVVRRTGQDVEWTDGRDVWWHDAVDSASKEHTPEAFDSEHPLYVMYTSGTTGKPKGIL
                     HTTAGYLVGCAWTHWAVFDLKDDDIFWTAADIGWVTGHSYIVYGPLANGATSVMYEGT
                     PDTPHKGRWWEIIQNYKVTLLYCAPTVIRTFMKWGADIPEKFDLSTLRIIGSVGEPIN
                     PEAYVWYRETIGGGRTPVMDTWWQTETGMHMISPLPGVTAGKPGSAMKALPGVSALVV
                     NDEGEAVKPGSGGYLVLTEPWPAMLRTIWGDDDRYQETYWSRFPGMYFAGDGAKLDED
                     GDVWLLGRVDDVMNVSGHRLSTTEIESALVSHP"
BASE COUNT          338 a          666 c          716 g          298 t
ORIGIN      
        1 tggcgtgggc gatcttcacg gcgtacatgt gcgtggcggc ggccagggtc agcacggcgg
       61 tgctcagcgt gttcgtgctg ctgacgatca cctacctcgc gctgacgatc gggtggttcg
      121 accaggagtc gagcggctgg atcaagctcg gcggatatct cgggatcctc accgccatcg
      181 ccgcgtggta cacctcgttc gccggagtca cggcgttcac ctggggtcga caggtcttcc
      241 cggtcggtcc gcgcaacgcg cctcgcgact gagcgcaccc gacgaccgga tcaccggtca
      301 ccgccttcgc gccgggacca cttcggtgga accatccgag tcctagggtg gttgtgggac
      361 cggcgcgaga accgtccgat aggagacgaa gtctgtgagt gaagaaaccc tgtccaacct
      421 gctgcacgag gagcgccggt tcgagcctcc cgccgacctg gccgcgaagg ccaacgtcac
      481 ggtggacgcc tacgacgagg ccgagagcga cttcgtggcg ttctgggagg gcgcggccga
      541 acggctcacc tgggacacca ggtggacccg tggcctcgac tgggacgacg cgccgttcgc
      601 caagtggtac gtcggcggca agctcaacgc ggcgtacaac tgcgtcgacc ggcacgtcga
      661 gaacggccac ggcgaccggg tcgcctacca ctgggtcggc gagcccgagg acgacacccg
      721 cgacatcacc tacgccgagc tcaaggacat ggtctgccag gccgccaacg ccctggtcga
      781 cctcggagtg aagacgggtg accgggtcgc gatctacatg ccgatgatcc ccgagaccgt
      841 ggtcgcgatg ctggcctgcg cacggctcgg cgccccgcac accgtggtgt tcggcggctt
      901 ctcgtcgacg gctctgcgcg accgggtcca ggactgcgac gcacggatcg tgatcactgc
      961 ggacggcggc taccgccgcg gcgcagcggc cgcgctgaag ccggcggtgg acgaggcact
     1021 gcagcagtgc ccggacgtcc gcaacgtgct ggtggtacgc cgtaccggcc aggacgtcga
     1081 gtggaccgac ggccgcgacg tctggtggca cgacgccgtg gacagcgcgt cgaaggagca
     1141 cacgccggag gcgttcgact ccgagcaccc gctctacgtc atgtacacgt cgggcacgac
     1201 cggcaagccg aaggggatcc tgcacacgac ggccggctac ctggtggggt gcgcgtggac
     1261 gcactgggcg gtcttcgacc tcaaggacga cgacatcttc tggaccgcgg ccgacatcgg
     1321 ctgggtcacc gggcactcct acatcgtcta cggaccgctg gcgaacggcg cgacgtcggt
     1381 gatgtacgag gggacgccgg acaccccgca caaggggcgc tggtgggaga tcatccagaa
     1441 ctacaaggtg acgctcctct actgcgcacc gacggtgatc cgcacgttca tgaagtgggg
     1501 cgcggacatc ccggagaagt tcgacctgtc gacgctgcgg atcatcggct cggtcggtga
     1561 gccgatcaac cccgaggcct acgtctggta ccgcgagacc atcggcggcg gccgcacccc
     1621 ggtgatggac acgtggtggc agaccgagac cggcatgcac atgatcagcc cgcttcccgg
     1681 cgtgaccgcg ggcaagccgg gctcggcgat gaaggcgctg ccgggtgtct ccgcgctggt
     1741 cgtgaacgac gagggagagg ccgtgaagcc gggctcgggt ggctacctcg tgctgaccga
     1801 gccgtggccg gcgatgctcc gcacgatctg gggcgacgac gaccgttacc aggagaccta
     1861 ctggtcacgc ttcccgggca tgtacttcgc cggcgacggc gcgaagctcg acgaggacgg
     1921 cgacgtgtgg ctgctcggcc gggtcgacga cgtgatgaac gtgtccggtc accggctctc
     1981 gacgaccgag atcgagtcgg ccctggtgtc gcacccga
//
LOCUS       DATBFZ010000080        12066 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_1036059, whole genome shotgun sequence.
ACCESSION   DATBFZ010000080 DATBFZ010000000
VERSION     DATBFZ010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 12066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12066
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1036059"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            171..1022
                     /locus_tag="VH419_02370"
     CDS             171..1022
                     /locus_tag="VH419_02370"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020386830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HEY7042492.1"
                     /translation="MLPFLAVAAAVPVAWGGWLGWTDLAIMAVMYVVTGLGITVGFHR
                     YFTHKSFKPNRGVKIALGAAGSMAIQGPIVRWVADHRKHHKFSDRDGDPHSPWRYGNT
                     VPALMKGLVYAHMGWLFDAEQTSQRQYAPDLLKDRDIVRMSRAFPWFVAASLLIPAIA
                     GAALTMSWQGALTAFFWASLVRIGLLHHVTWSINSICHAVGERPFKARDKSGNVWWLA
                     VPSFGESWHNLHHADPTCARHGVLRGQVDISARVIWILDKLGWIQAVRWPVKERLEMK
                     RAQPSDA"
     gene            1125..1775
                     /locus_tag="VH419_02375"
     CDS             1125..1775
                     /locus_tag="VH419_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020391368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY7042493.1"
                     /translation="MTDAKPVPKNKRNPRSRMTSAERREQLIEIARGLFATRGFEGTS
                     IEEIASRAEVSKPIVYEHFGGKEGLYAVVVDREVRQLLSMMRTSLTAGDPRDLLEQAA
                     FALLDYIEQSSDGFRILVRDSPIGSKTGSFVSIIGDIASRVEYILEEEFRNRGFDAKY
                     APMYAQMLVGMVGTTGQWWLEARKPTKNEVAAHLVNLAWNGLSGLEQRPTLHNPRG"
     gene            complement(1781..2812)
                     /locus_tag="VH419_02380"
     CDS             complement(1781..2812)
                     /locus_tag="VH419_02380"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017934748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEY7042494.1"
                     /translation="MTTEQELSGELEAFIAGLPKAELHVHHVGSASPRIVAELAARHT
                     DTPVPKDPELVAEYFTFTDFAHFVDIYLSVVDLVRTAEDIRMLTYEIARDMTEQQIRY
                     AELTCSPYTSVIRGIAPEAYCEALEDARLAAVRDFGIVLRWCFDIPGESGLPAADETV
                     RIATEIAPAGLVAFGLGGPEIGVPRPQFQPHFDRARAAGLHSAPHAGETTGPETIWDA
                     LRLLGADRIGHGTTATQDPELVSYLAEHRVPLEVCPTSNVATKAVPTLAAHPIRAMRD
                     AGIVVTVNSDDPPMFGTTLNREYAVAADLLDLDEDGVKALARSAVDVSFADEDVKKRL
                     VEEIDAYAS"
     gene            complement(2823..3284)
                     /locus_tag="VH419_02385"
     CDS             complement(2823..3284)
                     /locus_tag="VH419_02385"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY7042495.1"
                     /translation="MLIRDARPEDLEPLLGLLDEDAIREIAEDLSDLRPYAAALEEIL
                     ALPHSTVLVGEVDGELVATAQVTWQRRLMYGASLVCQVESVRVTSTERNGGLGTELMQ
                     WIIDDARRRGCARIELTSNAKRVDARRFYERLGFTPSHVGMKRYLGSSSQV"
     gene            complement(3278..4078)
                     /locus_tag="VH419_02390"
     CDS             complement(3278..4078)
                     /locus_tag="VH419_02390"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012923276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030798 - trans-aconitate
                     2-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HEY7042496.1"
                     /translation="MTVETATWNPATYLQFADERSRPFGDLLARVGAEAPRRVVDLGC
                     GPGQLTAGLAARWPQATILGVDSSPAMIDAAHEHATPNVRFEVADLRDWRPTEPADVV
                     VSNATLQWVPDHLELLSRLVEAVAPDGWFALQVPANFGEPSHRLLYQLAADPRFAAAT
                     TGLERAAAPDAQTYLTHLTRLGLVVDAWETTYLHVLTGPDPVFRWISGTGARPVLQAL
                     TPEQRTQFEAEYKALLREAYPNRGFGTVLPFRRTFVVAHRLERTPAGC"
     gene            4337..4834
                     /locus_tag="VH419_02395"
     CDS             4337..4834
                     /locus_tag="VH419_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008362597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY7042497.1"
                     /translation="MAAADEVDRLVEAWQRERPDLDVAPMRVLSRVTRLARHLDLARR
                     QAFAAHDLETWEFDVLSALRRAGTPYELSPGRLIRETMVTSGTMTNRVDRLSAKGLVA
                     RLPDPGDRRGVRVRLTDEGRHRVDAALDGLLEQERRLLHDLGPQDQGKLADLLRAVVA
                     PFDAP"
     gene            complement(4929..5831)
                     /locus_tag="VH419_02400"
     CDS             complement(4929..5831)
                     /locus_tag="VH419_02400"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007078449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEY7042498.1"
                     /translation="MTVTVRTPAKINLGLSVGPIREDGYHSVATVYQAISLYDEVRAR
                     PADDGVFTVSVTGEGQDLVPLDDTNLAVQAARMLAKAFDLDSGVALSIHKTIAVAGGL
                     AGGSSDAAGALVACDALWGTNASRDELWRIASALGADVPFCLTGGIAIGTGRGEHITP
                     VLARGGYEWVLAYADGTLATPEVYAELDRLRGSAAVPEPAASEVLMTALRSGDPYAVG
                     QALSNDLEEPALRLRPALRRTLDLGDEYGALGTLVAGSGPTCLYLAADEDHAVDLAVA
                     LSSSGLCRSVQRATGPVGGARLIT"
     gene            complement(5852..6721)
                     /gene="rsmA"
                     /locus_tag="VH419_02405"
     CDS             complement(5852..6721)
                     /gene="rsmA"
                     /locus_tag="VH419_02405"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020579766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEY7042499.1"
                     /translation="MDASPSASAGPRLLGPADVRALAAAYNVRPTKQRGQNFVIDANT
                     VRRIVRAADVDAHDEVLEVGPGLGSLTLALLDKVRHVTAVELDPGLAAALPETIRAFA
                     PHHVDRVTVVAGDAMRLDRVPGSEPTALVANLPYNVAVPVLLHLFATFPSIERVLVMV
                     QLEVADRLAAPPGSKVYGVPSVKAAWYADVRKAGLVGRNVFWPAPNVDSGLVAMVRRE
                     PPRTAAGRDAVFAVVDAAFAQRRKTLRAALASYAGSASAAEEAIRKAAVDPAARGERL
                     TIDDFARIADAFV"
     gene            complement(6769..7857)
                     /locus_tag="VH419_02410"
     CDS             complement(6769..7857)
                     /locus_tag="VH419_02410"
                     /inference="COORDINATES: protein motif:HMM:NF019142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase family protein"
                     /protein_id="HEY7042500.1"
                     /translation="MATLATVLAAGLALGTVAFASASKKSVTVSIDGRERVVKTSGDT
                     VADVLEDQGIDLGRHDAVAPSVSAKIDDGSRIAVSYGRELTLNIDGKKATYWTTATNV
                     QDALQQVGQRVAVGSDLSASRSSSIGRDGMRLVIRTPKRVVLKVGTHDAQKVTTTGIT
                     VTEALLDRGVEVDSLDVVRPGKQTRIDDGTRIVVIRRSSTRISAVESIPFGTIVRHDS
                     GMYDDQSDVLRSGHAGAERVSYQVMRRNGEVVHKRVVGHHTLRKPVPQIEVHGTKERP
                     VANYSSGSTVWDTIAQCESGGNWAINTGNGYYGGLQFSASTWTAYGGGAYASTANLAS
                     REEQIAIAEKVQAAQGWGAWPVCAAGVG"
     gene            complement(8171..9070)
                     /locus_tag="VH419_02415"
     CDS             complement(8171..9070)
                     /locus_tag="VH419_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012923289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEY7042501.1"
                     /translation="MTSSDGPLRERSGDSEQGRRDLARPPLPEPLPSPVVDNHCHLDI
                     VDGDSWLSPLEALELSRSVGVPRIVQIGCDLPGARWSVETATLHDTIVAGVSIHPNEA
                     AKLAAQGRLDEALAVIEMLAASSPRVRAVGETGLDYFRTPPDGRAAQQRSFVAHIEMA
                     KRLDKALVIHDRDAHDDVLALLDRHGLPERTVMHCFSGDADFARACLDRGAYLSFAGT
                     VTFKNAANLRRALAVAPLDRILVETDAPYLAPMPYRGRPNASYLVPHTMRCMAEVRQV
                     PLEDLCAAVDANTDAAFGGPWGR"
     gene            complement(9067..10878)
                     /gene="metG"
                     /locus_tag="VH419_02420"
     CDS             complement(9067..10878)
                     /gene="metG"
                     /locus_tag="VH419_02420"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEY7042502.1"
                     /translation="MSKVLSAVAWPYANGPRHIGHVAGFGVPSDVFSRYMRMAGHDVL
                     MVSGTDEHGTPILVTADQVGVSPRQLADENNRLIVEDLAGLGLSYDLFTRTTTRNHYA
                     VVQELFLTVYRNGYMIEQTTQGAISPSTGRTLPDRYIEGTCPICGYDSARGDQCDNCG
                     NQLEATDLINPRSKINGEAPEFVETQHFFLDLPALADTLSGWLDERAASGTWRPNVIR
                     FSQNILEGIRPRAMSRDIDWGIPIPLDGWRDLPTKRLYVWFDAVIGYLSASIEWARRT
                     GDPERWREWWNDPDALSCYFMGKDNIVFHAQIWPAELLAYNGKGSRGGNPGKYGELNL
                     PTEVVSSEFLTMEGKQFSTSRSVVIYVRDVLDRYQADALRYFICAAGPETSDSDFTWA
                     EFVRRTNGELVAGWGNLVNRTATMVHTNFGSVPEAGELTPDDVTLLAAVDAGFGSVGE
                     LLARHRFRQAIAEAMRIVGEVNKYVTAQEPYKLKAPEQRERLATILHVTTQAVVDCNT
                     MLAPFLPHAANRVHRVLGGEGDFVPMPRIEEVEDLDGGPGYPIITGEYADTPTWGRHV
                     IEPGRVVDKPTPVFTKLDPSVVDEELARLERDAGVSA"
     gene            complement(11029..11901)
                     /locus_tag="VH419_02425"
     CDS             complement(11029..11901)
                     /locus_tag="VH419_02425"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEY7042503.1"
                     /translation="MEIGLHIADFTWPGGTATMAQDLTRIVTTAEDVGIARISVMDHL
                     WQIGGIGPPENAMLEAYTTLGYLAAQTSRVKLLAWVTAAVYRHPGMLAKQVSTLDVLS
                     RGRAWLGIGAAWNKEEADGLGIPFPSTAERFERLEEALQICLQMWSDDDEPYDGRHYQ
                     LRRTLNSPQNITRPHPPILIGGGGERKTLRLVAKYAQACNLFETPELEHKLEVLRRHC
                     DDVGRDYDEIEKTVMGPIDPGDTGEDVDRLLERLQRLARLGVSEVHGQVRDVFDVKRL
                     ELLGERVIPVAATL"
BASE COUNT         1897 a         4134 c         4081 g         1954 t
ORIGIN      
        1 ccccgacgac cgacccgacc cccgccgatg tcacagccga tgtcgcagcc tcggatcgcg
       61 ccgacgcctc aaccacgacc gccacgctcg atccggtcga ccaggcgctg ggcaccctcg
      121 gcggcgacca gcagtcccgc gcggagcaga tcacgatcgg ggtcttcatc gtgttgccgt
      181 tcctggccgt cgcggcggcg gtcccggtgg cgtggggcgg gtggctcggc tggaccgacc
      241 tggccatcat ggcggtgatg tacgtcgtga ccggtctcgg gatcacggtc ggctttcacc
      301 ggtacttcac gcacaagtcg ttcaagccca accgcggggt caagatcgcc ctcggcgcgg
      361 ccggctcgat ggccatccag ggaccgatcg tgcgctgggt cgccgaccac cgcaagcacc
      421 acaagttctc cgaccgcgac ggtgacccgc actcgccgtg gcggtacggc aacacggtcc
      481 cggccctgat gaaagggctg gtctacgcac acatgggctg gctgttcgac gccgagcaga
      541 cgtcccaacg ccagtacgcg cccgacctcc tcaaggaccg cgacatcgtc cgcatgtccc
      601 gggcgttccc gtggttcgtg gccgcctcgt tgctgatccc ggccatcgct ggcgctgcgc
      661 tcaccatgtc gtggcagggc gcgctcactg cgttcttctg ggcctccctc gtccggatcg
      721 ggctcctgca ccacgtgacg tggtcgatca actcgatctg ccacgcggtc ggtgagcgcc
      781 cgttcaaggc tcgcgacaag tccggcaatg tctggtggct ggccgtcccg tccttcggcg
      841 agtcctggca caacctgcat cacgcggacc cgacctgtgc ccgccacggc gtactccgcg
      901 gtcaggtcga catctcggcg cgcgtcatct ggatcctgga caagctcggc tggatccagg
      961 cggtgcgctg gcctgtgaag gagcgcctcg agatgaaaag ggcccagccc tccgacgcct
     1021 gagtcgtcac accgatcttc cgcccggcga ccctgggttg cgacaagcct gtcctgagcc
     1081 tgtcgaaggg ctcaaccgcc gcaacagcag ggacaatggt tgacgtgacc gacgccaagc
     1141 cggtgcccaa gaacaagcgc aacccgcgat cgcggatgac cagcgccgaa cggcgcgagc
     1201 agctgatcga gatcgcccgc ggactgttcg ccacgcgcgg tttcgagggg acgtcgatcg
     1261 aggagatcgc atcccgggcc gaggtgtcga agccgatcgt ctacgagcac ttcggcggca
     1321 aagagggctt gtacgccgtc gtggtcgacc gcgaggttcg acagctgctc tcgatgatgc
     1381 gcacctcgtt gacggcgggt gatccgcgag acctcctcga gcaggccgcc ttcgcgctac
     1441 tcgactacat cgagcagtcg tctgacggct tccggatcct ggtacgggac tcgccgatcg
     1501 gctccaagac cgggtcgttc gtgtcgatca tcggcgacat cgcctcacgc gtggagtaca
     1561 tcctcgaaga ggagttccgc aaccgcggct tcgacgcgaa gtacgcaccg atgtacgcgc
     1621 agatgctcgt cggcatggtc ggcaccaccg gccaatggtg gctcgaggca cgcaaaccca
     1681 ccaagaacga ggtcgccgcg cacctggtga acctcgcgtg gaacggcctg tccgggctgg
     1741 agcagcggcc gacgctgcac aacccacgtg gctgagccgc tcagctcgcg taggcgtcga
     1801 tctcctcgac gagtcgcttc ttgacgtcct cgtcggcgaa cgacacgtcg acggcgctac
     1861 gcgccagggc cttgacgccg tcctcgtcga ggtcgagcag gtcggccgcg acggcatact
     1921 cacggttcag cgtggtcccg aacatcggcg ggtcgtcgga gttgacggtg acgacgatcc
     1981 cggcgtcgcg catcgctcgg atcgggtgcg ctgccagcgt cggaacagcc ttcgtggcga
     2041 cgttcgacgt cgggcagacc tcgagcggga cacgatgctc cgcgaggtag ctgaccagct
     2101 cagggtcctg ggtcgcagtg gtcccatggc cgatccggtc cgcgccgagc aggcgcagtg
     2161 cgtcccagat cgtctccggc ccggtcgtct ccccggcatg tggcgccgag tgcaacccgg
     2221 ccgcccgggc ccgatcgaag tgtggctgga actgcggtcg cggaaccccg atctccggcc
     2281 cgccgaggcc gaacgccacc agcccggccg gggcgatctc agtggcgatg cgcacagtct
     2341 cgtcggcggc cggcaggcct gactcgccag ggatgtcgaa gcaccaacgc agcacgatgc
     2401 cgaagtcccg aactgcggcg agtcgggcgt cttccaacgc ctcgcagtac gcctcgggcg
     2461 cgataccccg gatgaccgag gtgtacggcg agcacgtcag ctccgcgtac cggatctgct
     2521 gctccgtcat gtcccgagca atctcgtagg tcagcatccg gatgtcctcc gccgtacgga
     2581 ccaggtcgac gaccgacagg tagatgtcga cgaagtgagc gaagtcggtg aaggtgaagt
     2641 actccgccac cagctcggga tccttgggca ccggcgtgtc ggtgtgacgt gccgccagct
     2701 cggccacgat ccgcggggac gccgagccga cgtggtgcac gtggagctcg gccttcggga
     2761 gtccggcgat gaacgcttcg agttcgccgg acagctcctg ctcggtcgtc atcctcggga
     2821 ctctagactt gcgagctgct tccgaggtag cgcttcatgc cgacgtgaga cggcgtgaag
     2881 ccgagccgct cgtagaacct gcgggcgtcg acccgcttgg cgttgctcgt gagctcgatc
     2941 cgggcgcagc cacgccgtcg cgcgtcgtcg atgatccact gcatcagctc ggtcccgaga
     3001 ccaccgttgc gttccgtgga cgtgacgcgg acggactcga cctgacagac cagtgaggcg
     3061 ccgtacatca ggcggcgctg ccaggtgacc tgcgccgtcg cgaccagttc accgtccacc
     3121 tcaccgacga gaaccgtgct gtgcggaagc gcgaggatct cctcgagggc agcggcgtag
     3181 ggtctcaggt cggacaagtc ttcggcgatc tcccggatgg cgtcctcgtc gagcagcccg
     3241 agcaggggtt cgaggtcctc ggggcgggcg tcccggatca gcatccggcc ggggtcctct
     3301 cgagccggtg cgccaccacg aacgtacgcc ggaagggcag gaccgtgccg aacccgcggt
     3361 tggggtacgc ctctcggagc agcgccttgt actctgcctc gaactgcgtt ctctgctccg
     3421 gtgtcagggc ctgcagcacc ggccgcgcgc cagtaccgga gatccagcgg aacaccggat
     3481 ccggaccggt gaggacgtgc aggtacgtcg tctcccaggc atcgacgacg aggccgagac
     3541 gtgtcaggtg agtcaggtac gtctgggcgt ccggcgccgc agcccgttcg aggccagtgg
     3601 tcgcggcggc gaaccgtggg tcggcggcga gctggtagag cagccggtgg ctcggttcgc
     3661 cgaagttggc cggtacctgg agcgcgaacc agccgtcggg cgcgaccgcc tcgaccagtc
     3721 tggacagcag ctcgaggtgg tcgggaaccc attgcaaggt ggcgttggac accacgacgt
     3781 cggccggctc agtcggtcgc cagtcgcgca ggtcggccac ttcgaaccgg acgttcggcg
     3841 tggcgtgctc gtgggccgcg tcgatcatgg ccggcgacga gtcgacgccg aggatggtcg
     3901 cctgcggcca gcgggctgcg agtcccgcgg tcagctgtcc tggaccgcag ccgaggtcga
     3961 cgacgcgccg gggtgcctcg gccccgaccc gggcgagcag gtcgccgaac ggtctcgacc
     4021 gctcgtctgc gaactgcagg tacgtcgccg ggttccaggt cgcggtctcc accgtcacga
     4081 cacctccaag aaaatcttga cgtcaagaga catcctgcca aacccaatct ctcgacgtca
     4141 agatacctcg tccttccgtc gtgccgggtt cccgcgaatt gcgggaaagt ggccgctgac
     4201 aacggcgttt cggcctgacg tttcccgcat ttcgcggttc cccacagcgg cgggacggca
     4261 ccggggaggg gccatcacgt gagtgtctcc acgtcaagag tcttgacgcg gtcattcacg
     4321 ctctacactg acgcgcatgg cagcggcgga cgaggtcgac cggctcgtcg aggcgtggca
     4381 gcgtgaacgc ccggacctcg acgtcgcgcc gatgcgcgtg ctctcccggg tgacccgact
     4441 ggctcgccac ctcgacctgg cccgccggca ggcgttcgcc gctcatgacc tcgagacgtg
     4501 ggagttcgac gtgctctccg cgctgcgcag ggccggtacg ccgtacgagc tctcacccgg
     4561 acggctcatc cgggagacga tggtgacgtc cggcacgatg acgaaccggg tcgaccggct
     4621 gagcgcgaag gggctcgtgg cgcggctgcc cgaccccgga gaccgccgcg gcgtacgggt
     4681 gcggctcacc gacgaaggca gacaccgcgt cgacgccgcc ctcgacgggc tgctcgagca
     4741 ggagcggcgg ctgctgcacg acctcggccc gcaggatcag gggaagctcg cggacctgct
     4801 acgggccgtc gtcgcaccct tcgacgcgcc ctgagtcggg tcgaggtggc aatcggcgtt
     4861 cgacacgccg gcaaacttca acgaaagctg ccacctcggc gggaggcctg tgcaacctcg
     4921 gcgggaggtc aggtgatgag gcgggcacct ccgacgggac cggtggcgcg ctggacggaa
     4981 cggcagagac ccgagctgga cagcgccacc gccaagtcga cggcgtggtc ctcgtcggcc
     5041 gcgaggtaga ggcaggtcgg gcccgagccg gcgaccaggg tgcccaaggc gccgtactcg
     5101 tcgccgaggt ccagcgtccg ccgcaacgcc ggccgcagtc gcagtgcagg ctcctcgagg
     5161 tcgttggaca acgcctgccc gacggcgtac gggtcgccgg agcgcagtgc cgtcatcagc
     5221 acctccgacg cagccggttc gggaaccgca gcggaacccc ggagcctgtc gagctctgcg
     5281 tagacctcgg gtgtcgcgag cgttccgtcg gcgtacgcga gcacccattc gtagccgcca
     5341 cgggcgagaa ccggggtgat gtgttcgccg cggccggtcc cgatcgcgat gccgcccgtc
     5401 aggcagaacg gcacgtcggc acccagtgcc gacgcgatcc gccagagctc gtcgcgtgag
     5461 gcgttcgtgc cccagagcgc atcacaggcg acgagcgccc cggccgcgtc actgctacca
     5521 ccggccaggc cgccggcgac cgcgatcgtc ttgtggatgc tcagcgccac accggagtcg
     5581 aggtcgaacg ccttcgccag catgcgagcc gcctgcactg cgaggttcgt gtcgtcgagc
     5641 ggtacgaggt cctgcccctc accggtgaca ctgaccgtga agacgccgtc atcggccggc
     5701 cgcgccctga cctcgtcgta cagcgagatc gcctggtaga cggtggcgac cgagtgatac
     5761 ccgtcctcgc ggatcggacc cacgctgaga cccaggttga tcttcgccgg ggtacgcacg
     5821 gtgacggtca tggcgtcctc acgcaccaag gctagacaaa agcgtccgcg attcgggcga
     5881 agtcgtcgat ggtgagccgt tcgcctcgcg ccgccgggtc cacagctgcc ttccggatgg
     5941 cctcctcggc tgccgacgct gatccggcgt acgaggcgag cgctgcgcga agcgtcttgc
     6001 gtcgttgcgc gaacgctgcg tcaacgacgg cgaagacggc gtcccggccg gccgcggtcc
     6061 tcggtggttc acgtctgacc atggcgacaa gacccgagtc gacgttgggc gcgggccaga
     6121 agacgttgcg ccccaccagg cccgctttgc ggacgtcggc gtaccacgcc gccttcacgc
     6181 tgggcactcc gtagaccttg gagccgggag gcgcggccag ccgatcggcc acctcgagct
     6241 gcaccatgac cagcacgcgt tcgatgctcg ggaacgtcgc gaacaggtgc agcagcacgg
     6301 ggaccgcgac gttgtagggc aggttcgcga ccagcgccgt cggctcggag cccggaacac
     6361 ggtccagccg catcgcgtcg cctgcgacga cggtgaccct gtcgacgtgg tgcggcgcga
     6421 acgcgcggat cgtctccggc agcgcggcgg cgagtccggg atccagctcg acggccgtga
     6481 catgcctgac cttgtccagc agtgccagtg tcagcgagcc cagcccgggt ccgacttcga
     6541 ggacttcgtc gtgtgcatcg acgtcagccg ccctgacgat gcggcgaaca gtgttggcgt
     6601 cgatgacgaa gttctggccg cgttgcttgg tgggccggac gttgtacgcc gcggccaacg
     6661 cacgaacatc cgccggcccg agaagcctcg gaccggcgga tgccgaaggg ctcgcgtcca
     6721 catcggccac tttaggtgtg gcgacgtggt gcgcggggtc tgtgggcgct agccgacacc
     6781 ggcagcgcag acaggccagg caccccagcc ctgcgcggcc tggaccttct ccgcgatcgc
     6841 gatctgctcc tcgcgactgg cgaggttggc agtcgaggcg taggcgccgc cgccgtacgc
     6901 cgtccacgtg ctggcgctga actgcagtcc gccgtagtag ccgttgccgg tgttgatggc
     6961 ccagttcccg ccggactcgc actgcgcgat ggtgtcccac acggtgctgc cgctggagta
     7021 gttggccacc ggccgctcct tcgtaccgtg cacctcgatc tggggcaccg gcttgcgaag
     7081 cgtgtgatgg ccgacgacgc gcttgtggac gacctcaccg ttgcgacgca tcacctggta
     7141 gctgacgcgc tccgcacccg catggccgga gcggaggacg tctgactggt cgtcgtacat
     7201 gccgctgtcg tggcgcacga tggtgccgaa cgggatcgac tcgacggccg agatcctggt
     7261 ggagctgcgg cggatcacca cgatgcgggt gccgtcgtcg atccgcgtct gcttgccggg
     7321 ccggacgacg tcgaggctgt ccacctcgac cccgcggtcg agcagggcct ccgtcacggt
     7381 gattccagtg gtcgtcactt tctgtgcatc atgcgtgccg accttgagca cgacccgctt
     7441 cggcgtacgg atgacgaggc gcatgccgtc ccggccgatg gagctcgagc ggctggcgga
     7501 caggtctgat ccgacggcca cgcgctggcc gacctgctgc agggcgtctt gcacgttcgt
     7561 ggcggtcgtc cagtaggtcg ccttcttgcc gtcgatgttc aacgtcagct cgcggccgta
     7621 ggagacggcg atccgcgagc catcatcgat cttcgcgctg acggatggcg cgacggcgtc
     7681 gtgccggccg aggtcgatgc cttggtcttc gagcacgtcg gcgacggtgt cgccggaggt
     7741 cttcacgacc cgctccctgc cgtcgatgga gacggtcacg ctcttcttgc ttgcggaggc
     7801 gaaggcgacg gtcccgaggg ccagacccgc ggccaagacg gtggccagtg tcgccactgc
     7861 gatcttcctg gaggtattca gaacagctcc gaacgtcgta ctttcccggg ccacagggcg
     7921 gcggggtccg tggaccaccg aggagccgct ttgccctggt tcagtaccat atgagggcat
     7981 ccctgcttcc ggccaacaag tccacgacca tgccagaggc atcggaggcg aacaaatcct
     8041 tgcctccgtt cccaccgggc ggcgtgacaa acctcacgct ggaacctcag cgatcatcac
     8101 gaggcgtgac ggcgacacca catgttgtgg ttgcgcgccg cggggacccc accggtggtg
     8161 gtttgtccgg ctaccgaccc caaggtccgc cgaacgcggc gtcggtgttg gcgtcgacag
     8221 cggcgcagag atcctcgagc ggaacctgcc tgacctcggc catgcagcgc atcgtgtgcg
     8281 ggacgaggta cgacgcgttg ggcctccccc gatacggcat cggcgcgagg tacggcgcat
     8341 cggtctcgac aaggatcctg tcgagcggcg cgaccgccaa cgcgcggcgc aggttggcgg
     8401 cgttcttgaa ggtcaccgtg ccggcgaacg acaggtaggc gccccggtcc aagcaggcgc
     8461 gcgcgaagtc cgcgtcaccg gagaagcagt gcatcacggt ccgctccggg aggccgtgcc
     8521 ggtcgagcaa cgcgagcacg tcgtcgtgcg cgtcccggtc gtggatcacc agcgccttgt
     8581 cgagccgctt cgccatctcg atgtgcgcga cgaacgagcg ctgctgcgcc gcgcggccgt
     8641 ccggcggcgt gcggaagtag tccaagccgg tctcaccgac agcgcgcacg cgtggactcg
     8701 atgcagcaag catctcgatc acggccagcg cctcgtcgag gcgaccttgc gcggcgagct
     8761 tcgctgcctc gttcggatgg atcgaaacgc ccgccacgat cgtgtcatga agtgtcgcgg
     8821 tctccacgga ccaccgggcc ccgggcaggt cgcagccgat ctgcacgatg cggggaacac
     8881 cgacgctccg cgacagttcc agcgcctcga gcggagacag ccagctgtcg ccgtcgacga
     8941 tgtcgagatg acagtggttg tcgaccaccg gactcggcaa tggctctggc agcggcggcc
     9001 gggcaaggtc gcgacggccc tgctcgctgt ctcccgaccg ctcccgcaac gggccgtcgc
     9061 tcgacgtcac gccgagaccc cggcgtccct ctcgagccgg gccagctcct cgtcgaccac
     9121 ggagggatcg agcttggtga agaccggcgt cggcttgtcc acgacccgac cgggctcgat
     9181 gacgtgtcgg ccccacgtgg gcgtgtcggc gtactccccg gtgatgatcg gatagccagg
     9241 cccgccgtcg aggtcctcga cctcctcgat ccgcggcatc ggcacgaagt cgccctcacc
     9301 gcccagcacc cgatgcaccc ggttcgccgc atgcgggagg aacggcgcga gcatcgtgtt
     9361 gcagtcgacc accgcctgtg tcgtgacgtg caggatcgtc gcgagccgtt cacgctgctc
     9421 aggcgccttg agcttgtagg gctcctgcgc ggtgacgtac ttgttgacct caccaacgat
     9481 ccgcatcgcc tcggcgatcg cctgccggaa gcggtgccgg gcgagcagct cgccgaccga
     9541 tccgaagccc gcgtcgacgg ccgcgagcag cgtcacgtca tcgggggtga gctcgccggc
     9601 ctccgggacc gagccgaagt tcgtgtgcac catggtggcc gtccggttga cgaggttgcc
     9661 ccagccggcc acgagctcac cgttggtacg ccgcacgaac tccgcccagg tgaagtcgct
     9721 gtcggatgtc tcgggcccgg cggcgcagat gaagtagcgg agcgcgtcgg cctggtagcg
     9781 gtcgagcacg tcacggacgt agatcaccac gctgcggctg gtcgagaact gcttgccctc
     9841 catcgtgagg aactccgagc tgacgacctc ggtcggcagg ttcagctcgc cgtacttgcc
     9901 gggattcccg cctcggctcc ccttcccgtt gtatgcgagc agctcggccg gccagatctg
     9961 ggcatggaag acgatgttgt ccttgcccat gaagtagcac gacagcgcgt ctgggtcgtt
    10021 ccaccactcg cgccagcgct cgggatcgcc tgtacgacgc gcccactcga tcgaggcgga
    10081 caggtagccg atgaccgcgt cgaaccacac gtagagccgt ttggtgggca ggtcgcgcca
    10141 cccgtccaag gggatcggga tcccccagtc gatgtcacgc gacatggcgc gcggccggat
    10201 cccttcgagg atgttctggc tgaacctgat gacgttcggg cgccaggtgc ccgaggcggc
    10261 gcgctcgtcg agccagccgc tcaacgtatc ggcgagtgcc ggcagatcga ggaagaagtg
    10321 ctgcgtctcg acgaactccg gcgcctcgcc gttgatcttg ctgcgcgggt tgatcaggtc
    10381 cgtggcctcg agctggttgc cgcagttgtc gcactggtca ccgcgcgcac tgtcgtagcc
    10441 gcagatcgga caggtgccct cgatgtagcg gtcggggagg gttcgacccg tcgacggcga
    10501 gatcgcgcct tgcgtcgtct gctcgatcat gtagccgttg cggtacacgg tgagaaacag
    10561 ctcttgcacg accgcgtagt ggttgcgcgt cgtcgtccga gtgaagaggt cgtacgacag
    10621 accgaggccg gccaggtctt cgacgatgag ccggttgttc tcatcggcca gctggcgcgg
    10681 tgagacaccg acctggtcgg ctgtcaccag gatcggcgta ccgtgctcgt cggtcccgct
    10741 gaccatgagc acgtcgtgtc ccgccatccg catgtaccgg ctgaagacgt cggacgggac
    10801 gccgaaaccg gccacgtggc cgatgtggcg cggcccgttc gcatacggcc aggcgacggc
    10861 ggacaagacc ttgctcatgg ccgcaatcct agattgactg tcgaaccggt ttcggcgtcg
    10921 tcccgctggc tgccgggtct ccgcgaattg cgggaaaagt caggcccaga cggcgtacga
    10981 cggagccggt ttcccgcaat tccgggtgac ccgagcggac ctgccgcgtc agagggtggc
    11041 ggcgaccgga atgactcgct cgccgagcag ctccaaccgc ttcacgtcga agacgtcacg
    11101 cacctggccg tgcacctccg agacaccgag ccgtgccagc cgttgcaacc gttcgaggag
    11161 cctgtcgacg tcttcaccgg tgtcacccgg gtcgatcggg cccatcaccg tcttctcgat
    11221 ctcgtcgtag tcgcggccga cgtcgtcgca atgccgccgc aggacctcca gcttgtgctc
    11281 gagctcaggt gtctcgaaga ggttgcacgc ttgcgcgtac ttcgccacga gccgcagcgt
    11341 cttccgctcg ccaccaccgc cgatgaggat cggtgggtgc ggccgcgtga tgttctgtgg
    11401 tgagttcagc gtgcggcgaa gctggtagtg ccggccgtcg tacggctcgt cgtcgtcgct
    11461 ccacatctgc agacagatct gcagtgcctc ctccaaccgc tcgaaccgct cagccgtcga
    11521 cgggaacgga ataccgagcc cgtccgcctc ctccttgttc caggcagcac cgatgccgag
    11581 ccatgcccgg cctcgtgaca acacgtcgag tgtggagacc tgcttggcca gcatccccgg
    11641 atggcggtac acagcggcag tgacccaggc gagcagcttg acacgggacg tctgcgcggc
    11701 caggtagccc aacgtcgtgt aggcctcgag catcgcgttc tcgggcggtc cgatcccgcc
    11761 gatctgccac agatggtcca tgacgctgat ccgtgcgatc ccgacgtcct ccgccgtcgt
    11821 gacaatcctg gtcaggtcct gggccatcgt cgccgtgccg ccgggccagg tgaagtcggc
    11881 gatgtgcagc ccgatctcca tgtgcaggtc tcctttgcca gaccccctcg ctcttcacgc
    11941 tagtccgagg ttcgacacat tgcccgacgc gatctcacgg ttcatgaggc cgtaactcac
    12001 ggttcaagga accacaactc acggctcgac gaaccacaac tcacggctcg acgaaccaca
    12061 actcac
//
LOCUS       DATBFZ010000081         2983 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_707952, whole genome shotgun sequence.
ACCESSION   DATBFZ010000081 DATBFZ010000000
VERSION     DATBFZ010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2983
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707952"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="VH419_02430"
     CDS             <1..258
                     /locus_tag="VH419_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012920910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEY7042504.1"
                     /translation="RRSVQIVVGVTLLVSGALVLFNQTYLEPYDSATGQVVLMAVIGL
                     FALGVFWLRRLAGVEVPERFLVQSGRRGSERLDVVVGRGRA"
     gene            255..1157
                     /locus_tag="VH419_02435"
     CDS             255..1157
                     /locus_tag="VH419_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020384795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEY7042505.1"
                     /translation="MISVMLAGALAGLGVFLLVRVFVRPKPGVAATLARIDAGRRASV
                     PAAAVTETAKRPSWLERTRLSVGSRLEAEAAARGWRFDKTKTDLAIMNRPFAVHLGSK
                     VLLGLGALLWAPAVLTLVDLGPTISGILTLACSVFAFFIPDLALRKEAERRRRDFRHV
                     VGSFLDLVAMNLAGGRGLPEALMAASSVGDHWAMARIRQALSNARVIGITPWEGVSRL
                     GRELGVEELRDLASALALAGDEGAKIRLSLMARAESMRRKELADVEGQAGERSQSMLV
                     AQLLICASFLLFLAYPATSQILNS"
     gene            1306..1530
                     /locus_tag="VH419_02440"
     CDS             1306..1530
                     /locus_tag="VH419_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042506.1"
                     /translation="MPAEFRLVQQLLQYAALRREALRYRKDDHWGASAVEWAIISAIV
                     VAAAVLIGRAIFSLVSNKKNEMCQESEGGC"
     gene            1582..2001
                     /locus_tag="VH419_02445"
     CDS             1582..2001
                     /locus_tag="VH419_02445"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE family protein"
                     /protein_id="HEY7042507.1"
                     /translation="MTVLRRRRDQRGASAIEMAIVAPSFLLFIFAIVEVALWMHARDV
                     AIAAAREGVATLRTVNTQDSPSRWETYVERKAEQTANDLGDIQDPSADAAYDDDNKRV
                     TVTVKGGVIDLIPGWDLSVSGTASGLIEDFQPDLGEP"
     gene            2013..2489
                     /locus_tag="VH419_02450"
     CDS             2013..2489
                     /locus_tag="VH419_02450"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE family protein"
                     /protein_id="HEY7042508.1"
                     /translation="MNDQRGSSSLELVFLAPVLLSVMLLVAAFGRHAHTQAYIDQAAR
                     DAARTATATRDAATAKQAVNDTIEADLASAPPSCRDSAEHTVTSSTGSFEPSSPYTAY
                     PIGDEPGLVVLSVTVTCDVDVSDLSFIGIGHLTLTSTFSSPMPALYGVYCNPICGF"
     gene            2493..>2983
                     /locus_tag="VH419_02455"
     CDS             2493..>2983
                     /locus_tag="VH419_02455"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HEY7042509.1"
                     /translation="MTETTKDRGHHRHPRRALWSTRRRLLSRRRRDQRGVLTPAVVVM
                     AIGLLLLGGLVTDGGRQLNARSRAQATAEEAARAAADMLDLREAEAVIDKDKATDAVN
                     RFCEVARQEDDSITACEVADIGHDDNTDADFVKVHVQITITPLLFGLIGVGDMHADVT
                     ASAS"
BASE COUNT          441 a         1032 c         1024 g          486 t
ORIGIN      
        1 cgtcgcagcg tgcagatcgt ggtcggcgtc acccttctcg tgtccggtgc gttggtgctg
       61 ttcaaccaga cctacctgga gccgtacgac tcggcgaccg ggcaggtcgt actcatggcc
      121 gtcatcgggc tgttcgcgct cggggtcttc tggctgcgcc ggctggccgg ggtggaagtg
      181 cccgaacgct tcctcgtgca gagcgggcga cgtgggtccg agcggctcga cgtcgtggtc
      241 gggaggggcc gggcatgatc agcgtgatgc tcgccggcgc cctcgccggc ctgggtgtct
      301 tcctcctggt gcgcgtcttc gtccgtccca agcccggggt ggcggccacg ctggcgagga
      361 tcgacgcggg acgacgcgcg tcggttccgg ccgcggcggt caccgaaacg gcgaaacggc
      421 cgagctggct ggaacgcacc aggctgagcg tcggctcccg actggaggcc gaggcggctg
      481 ctcgggggtg gcggttcgac aagacgaaga cggatctggc gatcatgaac cggccgttcg
      541 ccgtccacct cggcagcaag gtgctcctcg ggctcggggc cttgctgtgg gcgccggccg
      601 tactgaccct cgtcgacctc ggcccgacca tctccggcat cctcacgctc gcgtgctcgg
      661 tcttcgcgtt cttcattccc gaccttgccc tccgcaagga agccgagcga cggcgtcgcg
      721 acttccggca cgtcgtcggc tcgttcctcg acctggtggc gatgaacctc gctggcggcc
      781 gcggtctgcc ggaagcgttg atggccgcat cctcggttgg cgaccactgg gcgatggcgc
      841 gcatcaggca ggcgctgagc aacgcccgcg tcatcggcat cacgccgtgg gagggtgtga
      901 gccggctcgg gcgggagctc ggggtcgagg agctgcgtga cctcgcgtcc gcgctggcgc
      961 tggccggtga cgagggcgcg aagatccggc tctcgctgat ggcgcgcgcg gagtcgatgc
     1021 ggcgcaagga gctcgccgac gtcgagggac aggcgggtga gcggtctcag tccatgctcg
     1081 tcgcccagct gctgatctgc gcgtcgttcc tgctcttcct cgcctacccg gcgacctccc
     1141 agattctcaa cagctgagtc agtcgatgag tcagcacatg tccgggtccg ccgccattcc
     1201 gcccgaagcg cgcatcaccg cgcacgtcat cacgtatcgt ccgtcccgca tggcagccca
     1261 tgcgaggccg gtgacagtgg ccagttcagt cgaagggatc tccacatgcc agcagagttc
     1321 aggctcgtcc agcagctgct gcagtacgcc gcgctgcgac gcgaggcgct gcgctaccgc
     1381 aaggacgacc actggggcgc gtcagcggtc gagtgggcca tcatctccgc gatcgtcgtc
     1441 gccgctgccg tgctcatcgg ccgggcgatc ttctcgctgg tctccaacaa gaagaacgag
     1501 atgtgccagg agtccgaagg cggctgctga cgtcgccgcc cgccgctgca gcggaccgac
     1561 cgaccgtcct gaccggggcc gatgaccgtg ctgcgccgtc gtcgagatca gcggggggcc
     1621 agcgccatcg agatggcgat cgtggccccg tcgttcctgc tgttcatctt cgccatcgtc
     1681 gaggtcgcct tgtggatgca cgcgcgcgac gtcgccatcg cggcagcgcg cgaaggcgtg
     1741 gccaccttgc gcaccgtgaa cacccaggac tcgccgagtc gctgggagac ctacgtcgag
     1801 cggaaggcgg agcagacggc gaacgacctc ggtgacatcc aggatccgtc ggccgacgcg
     1861 gcgtacgacg acgacaacaa gcgtgtcacc gtgaccgtga agggcggagt gatcgatctc
     1921 atccccgggt gggacctgtc ggtgtccggc acggcgtctg gtctgatcga ggacttccag
     1981 cccgacctgg gggagccgtg aggatcctcc gggtgaacga ccagcgcggg agctcgtcgc
     2041 tcgagctggt gttcctcgca cccgtcctgc tgtcggtcat gctgctcgtc gccgccttcg
     2101 gccggcacgc gcacacgcag gcgtacatcg accaagctgc gcgggatgca gcccgaacgg
     2161 ccacggcgac ccgcgacgcg gcaacggcca agcaggcggt caacgacacg atcgaggccg
     2221 acctggccag cgctcctccg tcgtgccgcg actccgcaga gcacacggtg acctcgtcga
     2281 ccggctcgtt cgagccgtcg agcccctaca ccgcgtaccc gatcggcgac gagccgggcc
     2341 tggtcgtcct ttccgtgacc gtgacctgcg atgtcgacgt gtccgacctg agcttcatcg
     2401 gcatcggcca cctgaccctg accagcacgt tctccagccc gatgccggcg ctgtacggcg
     2461 tttactgcaa cccgatctgc ggattctgag tgatgaccga gacgacgaag gaccgtggcc
     2521 accacaggca cccccggcgt gccctgtggt cgacgcgtcg gcgcctgctg tctcgtcgcc
     2581 gccgcgacca gcgcggtgtc ctcaccccgg cggtcgtcgt gatggccatc ggtctcctgt
     2641 tgctgggtgg cctcgtcacc gatggcggac gccagctcaa cgccaggtcc cgggcccagg
     2701 cgacagccga agaggcggcc cgcgccgccg cggacatgct cgacctacgc gaggccgagg
     2761 ccgtgatcga caaggacaag gcgacggatg cggtcaaccg cttctgcgag gtggcccgtc
     2821 aggaagacga ctccatcacc gcatgcgagg tcgccgacat cggtcacgac gacaacaccg
     2881 acgcggactt cgtcaaggtg cacgtccaga tcacgatcac gccgctgctg ttcggtctca
     2941 tcggggtcgg cgacatgcac gccgacgtga ccgcgtcggc atc
//
LOCUS       DATBFZ010000082         6657 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_381660, whole genome shotgun sequence.
ACCESSION   DATBFZ010000082 DATBFZ010000000
VERSION     DATBFZ010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 6657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6657
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381660"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            86..445
                     /locus_tag="VH419_02460"
     CDS             86..445
                     /locus_tag="VH419_02460"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEY7042510.1"
                     /translation="MRLTEQQTDRACGVLLGAACGDALGAGYEFGSSPTPPPGRRPDM
                     IGGGLGGFALGEWTDDTAQTYAIASVAATGADLRTPTALDAIAAGFARWFAEGPADVG
                     ILTREVLGTAGPDATAR"
     gene            445..1083
                     /locus_tag="VH419_02465"
     CDS             445..1083
                     /locus_tag="VH419_02465"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEY7042511.1"
                     /translation="MTAAARRVHDRLGRSGGNGSLMRTSPVALAHLDDPKALVTAAGA
                     VSALTHTDPQAGEACALWCLGIRSAVLDAVFPDLRDGLALLPKDSRPFWLGKIEEAER
                     LEPRRFRPNGYVVTALQAAWSAINLTQVIETRGEEGIGQGHILDALETAIRIGDDTDT
                     VASIAGALLGARWGASAFPSTWRSLVHGWPDATAEDLVDLARWSVGFRSLPE"
     gene            complement(1124..1603)
                     /locus_tag="VH419_02470"
     CDS             complement(1124..1603)
                     /locus_tag="VH419_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009078620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03668 family PPOX class F420-dependent
                     oxidoreductase"
                     /protein_id="HEY7042512.1"
                     /translation="MDERQMRHLVATAPVGRLATVDADGRPHLVPVCFALLDTTAYSA
                     IDHKPKTTTQLRRLQNLRDTGVACLLVDHYEADWSRLWWVRLDGVGRVLASGAEPGSD
                     QHTERQQALDALSTKYQQYTERPPTGPVLALDVTTWRGWSAQPAGSATSSTANDCWT"
     gene            complement(1614..2195)
                     /locus_tag="VH419_02475"
     CDS             complement(1614..2195)
                     /locus_tag="VH419_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020390745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY7042513.1"
                     /translation="MSDYPVKALDSDTWGAFERLAERHNGVFGGCWCTWFQTLPADKT
                     FTADGNHALKRRLVDEGRAHAALVFDGDEAVAWCEYGSPDELPNIYHRKQYEAELDRL
                     PDYRITCMFVDKKYRRKGLTAVALRGALDLISQAGGGVVEGYPHDTEGKKVAVLYNGT
                     RSLFERAGFTYVRPKGMKNCVMRIEVPKPRSRR"
     gene            2383..2607
                     /locus_tag="VH419_02480"
     CDS             2383..2607
                     /locus_tag="VH419_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042514.1"
                     /translation="MSKIHARIAAVLFALAAVFTISIGTASTADASAADCHDGVILWN
                     YYMSIGDYATAHGLLSNLIAMGCADVELES"
     gene            complement(2656..3084)
                     /locus_tag="VH419_02485"
     CDS             complement(2656..3084)
                     /locus_tag="VH419_02485"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEY7042515.1"
                     /translation="MSSRLKGRRRQVSAVIDALIAWAKAQPDVTAVALVGSYAYDRPR
                     MGSDVDLVLLTTSPDRHTEGLGWALAVDPRARLIRDRAWGPLRERRVRLRSGLQIELG
                     IVTPEWAALPLDEGTATVLRDGCRILFDPDGVLDRAIATL"
     gene            3180..5864
                     /locus_tag="VH419_02490"
     CDS             3180..5864
                     /locus_tag="VH419_02490"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY7042516.1"
                     /translation="MVALPTGSVTLLFSDIEGSTLLVRRLGSDWTDALDSQRRLLREA
                     WTCHDGVELGTEGDSFFVAFDAAAAGVQAAVAAQRAMAHHAWPQGEHVVVRMGLHTGT
                     PLVHDGGYVGLDVHRAARIAAAAHGGQVLVSEATKSLASRGVAEELRFTDLGRHRLKD
                     LLEPERLFQVQGDDLRATFPPVRSLGAPSRLPTTADRLVGRDAELVELTRLLSGAEAR
                     LVTLTGPGGSGKTSLGVALARALAVAPQEAGQVPVFPDGVFFVPLAEVTGVAEMWRMI
                     GEALAVPNVRRNPSGVVAEVEQWRALLVLDNLEQLVGADEVVDVLLRETTSLALLTTS
                     RQPLHVAGEYDHPVPALDVPADDSRAAVNASAAAQMFTDCVRRVQPAFELTDLNAGPI
                     AQICRRLDGLPLALQLVAARSKVLGPKAIAARLDQALDVVSNQRSASPRQHTLRETIA
                     WSYRLLDEEQRRILRGLGVFAGGGDLDAVAAVCGEPTTTRSSDGLLEALSSLVDASLV
                     DVVEAADGEPRFTLLETIRAFARDEMRGAGELQAIQERHASHFVDEAEALAATINGPG
                     FVSVVGQFAVDYDNFREALAWATTPGSSTSPGHDANALGLRLIAALMRYWRLAGLNAE
                     DKWWTERILEVAAAVDSAAYGRVLERLAVHRMSTNPAEAKELARKVVEIGRRVNDERL
                     VANSLVTLGDIHIQVEGDREGGIALYKEAWPHAQRSEDADAQANVLEGLCEVSTLSGD
                     FEGALTQVAREEELIRARDNPLDRLRHEHMVAWVQRRAGRPEQALDWIQRAVRSALAI
                     GFAYANGMTYVAEDYACILADLGQAADAARLLGAADARRERIDAPRAPYVEADIEPSI
                     SKARAVLTAREWEDAYGEGRQQQLEDALTVAHRRTEAM"
     gene            5861..6043
                     /locus_tag="VH419_02495"
     CDS             5861..6043
                     /locus_tag="VH419_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042517.1"
                     /translation="MKAVVYDRYGSPDMLRVEDVPMPSSVAGQVLVNVAATSIKLSDE
                     LSELRAPGSPAEGLLQ"
     gene            complement(6003..6575)
                     /locus_tag="VH419_02500"
     CDS             complement(6003..6575)
                     /locus_tag="VH419_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020390350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HEY7042518.1"
                     /translation="MTSTYGFTPGHPPVWDIERFMRGDEVDTAVITLERNRRTFAWKC
                     ADVGAEGLGLRLGASSVTLGGLLKHLAWVEELYFQNRLAGRQSMAPFDQLDPERDWEG
                     WAWSTAADDTPDALRALWVDTVHRSRDALAEVLSCGGLEQTTSNGMSLRRLLADVIEE
                     YARHTGHADLLREQVDGATGEGPPQDFPVP"
BASE COUNT         1076 a         2158 c         2316 g         1107 t
ORIGIN      
        1 cgcgcgattg cggggacttc cgggatttcg cggctgagaa acccggaacc tcccgcagtt
       61 cgtgaagcct gggaggctga gcgggatgcg gctgacagag caacagacgg atcgggcgtg
      121 cggcgtactc ctcggagcgg cgtgcggcga tgcgctcggc gcggggtacg agttcggtag
      181 cagcccgact ccaccgccgg gacgaaggcc ggacatgatc ggaggcgggc tgggtgggtt
      241 cgcgctaggt gagtggacgg atgacacagc acagacgtac gcaatcgcgt cggtcgccgc
      301 cacaggtgcc gatctgcgta cgccgactgc gctggatgcg atcgccgccg gctttgcccg
      361 gtggtttgcc gaaggccctg ccgacgtggg catcctgacg cgcgaggtgc tgggcactgc
      421 cgggcctgat gcgacagcga ggtgatgacg gccgctgctc ggcgggtcca cgaccggctg
      481 ggacgcagtg gcggcaacgg atcgctcatg cgaacctccc cggtcgcact ggcgcatctc
      541 gacgacccga aggcgctcgt gaccgcggcc ggcgcggtga gcgccctgac ccacaccgac
      601 ccgcaggcgg gagaggcctg tgcactctgg tgcctgggca tccggtcagc cgtgctggac
      661 gctgtctttc ccgacctgcg cgacggactc gcgctgctgc ccaaggactc ccggccgttc
      721 tggctgggaa agatcgaaga ggcggagcgg ctggaaccca gacgcttccg accgaatggg
      781 tacgtcgtga ccgccctcca agcagcctgg tcggcgatca acctgacaca agtgatcgaa
      841 acgcggggtg aggaaggcat cgggcaaggg cacatcctcg acgcgctgga gaccgccatc
      901 cggatcggcg acgacaccga cacggtcgcg tcgatagccg gtgccttgct cggtgcgaga
      961 tggggcgcga gtgcctttcc ctcgacgtgg cggagtctcg tgcacgggtg gcctgacgcg
     1021 acagcggagg acctggtgga cctcgcacgc tggtcggtcg ggttccgtag cttgccggaa
     1081 tgacgaatga ccgttccaga tccgtcgttc gccgagggat caactacgtc cagcagtcgt
     1141 tggcggtcga gctcgttgct gagcccgctg gttgggcgga ccagcctcgc caggtggtga
     1201 catcaagcgc caggaccggt ccggttggcg gtctctcggt gtactgctgg tacttcgtcg
     1261 acaacgcgtc cagcgcctgt tgacgttcgg tgtgctgatc ggaccccggc tcagcaccgc
     1321 tcgcaaggac gcgaccgacg ccgtcgaggc gtacccacca cagtcgtgac cagtcagcct
     1381 cgtagtggtc gacgagcagg caggcgacgc cggtgtctcg gaggttctga aggcgtcgaa
     1441 gttgcgtcgt cgtcttcggc ttgtggtcga tcgcgctgta ggccgtcgtg tccagcagtg
     1501 cgaagcacac cggaacgagg tgcggccggc cgtcggcgtc gaccgttgcg agacgcccaa
     1561 ccggggcagt ggcgaccagg tgtcgcatct gccgctcgtc cacccgttgg aggctaccgc
     1621 cgtgagcgcg gcttggggac ctcgatgcgc atcacgcagt tcttcatgcc tttgggtcgg
     1681 acgtaggtga agccggcccg ctcgaagagg ctccgggtgc cgttgtagag gaccgccacc
     1741 ttcttgccct cggtgtcgtg cgggtaccct tccacgaccc caccgccggc ttgcgagatc
     1801 aggtcgaggg cgccacgaag ggcgactgcc gtgagccctt tccttcggta cttcttgtcc
     1861 acgaacatgc aggtgatccg gtagtcgggc aacctgtcca gctccgcttc gtactgcttg
     1921 cggtggtaga tgttcggcag ttcgtcaggg ctgccgtact cgcaccaggc cacggcctcg
     1981 tcaccgtcga acaccagcgc ggcgtgcgcc cggccctcgt cgaccagccg ccgcttgagg
     2041 gcgtggttgc cgtccgcggt gaacgtcttg tctgccggga gggtctggaa ccaggtgcac
     2101 cagcagccac cgaacacacc attgtggcgc tcagcgagcc gctcgaacgc gccccacgtg
     2161 tccgaatcca gggccttgac cgggtagtcg ctcatcgggc tcctccttct ggccacaccg
     2221 tagaccgggt tgcggacgtt ctgtgtccgg aacttcggat ccggcatctc ggtgacaacg
     2281 acccatctcg tgatcgcttc cgttccgaat cccttgacga gtgcgcccac gagaacccgg
     2341 cggcgcggac cggggcgccc ggaacgaagt gagggggaca ccatgtccaa gattcacgca
     2401 agaatcgcgg ctgtgctgtt tgcactggcc gccgtcttca ccatctcgat cggaacggcc
     2461 agcacggccg acgccagcgc ggcggactgc cacgacggcg tcatcctctg gaactactac
     2521 atgagcatcg gggactacgc gacggcccac gggctcttgt cgaacctgat tgcgatgggt
     2581 tgcgcggacg tggaactgga gagctgaggc acgccgctga tgtgctgcgt ccggcgctga
     2641 ctcgggagcg tgcggtcaaa gggttgcgat cgcgcggtcg aggacgccgt cgggatcgaa
     2701 gaggatccgg cagccatctc gcaggaccgt tgcggtgccc tcgtcaaggg ggagcgccgc
     2761 ccactccggc gtcacgatgc ccagctcgat ctgcaggcca cttcgcagcc ggacgcgccg
     2821 ttctcggagt ggaccccacg ctctgtcacg gatgaggcgc gcacggggat cgacggccag
     2881 agcccagcca aggccctcgg tatgtcgatc ggggctcgtc gtgagcagga caaggtcgac
     2941 gtcgctcccc atgcggggtc ggtcgtaggc gtaggagccg acgagcgcca cggccgtgac
     3001 atcaggctga gccttcgccc aggcgatcag ggcgtcgatc accgcactga cttggcggcg
     3061 acgccctttc agcctggagc tcacgcggcc agcctctcag tcgcgtggga ccgatctgcc
     3121 gcggcgttgg gtgccgtgcc cgcgtggacg ttccgcagag gcgggtgtaa ccagggagca
     3181 tggtcgcatt gccgactggc agcgtgaccc tgctgttctc ggacatcgag ggttccaccc
     3241 tgctggttcg tcgcttgggg tcggactgga cggatgccct cgacagtcaa cgtcgtctgc
     3301 tgcgcgaggc ctggacatgc catgacgggg tggagctggg aacagagggg gacagcttct
     3361 tcgtggcgtt cgatgcggcc gcggccggcg tacaggcagc ggtggcggcc cagcgggcca
     3421 tggcgcacca cgcctggccg cagggcgagc atgtggtcgt ccggatgggc ttgcacaccg
     3481 ggacgccact ggtgcacgac ggcggctacg tcggcctgga tgtccaccgc gccgcgcgaa
     3541 tcgcagccgc agcgcatggc ggccaggtct tggtgtctga agcgaccaag tcgttggcgt
     3601 cccgaggtgt tgcggaggag ctacggttca cggatctggg ccgacatcgg ttgaaggatc
     3661 tcctggagcc cgaacgactc ttccaggtgc agggggacga cctccgtgcc acgttcccgc
     3721 cagtcaggag cctcggcgct ccgtccagac tgccgacaac cgcggaccga ctggtcggtc
     3781 gtgacgccga gctggtcgag ctcacgaggc tgttgagtgg cgccgaggcg cgtttggtga
     3841 cgctgaccgg acccggtggc tcgggcaaga cctcgctcgg ggtggcgctc gcgcgagcgc
     3901 tcgctgttgc tccacaggag gcaggtcagg tgccggtgtt tccggacggg gtgttcttcg
     3961 tgccattggc cgaggtcacc ggcgtcgccg agatgtggcg gatgatcggt gaggccctgg
     4021 ccgttccgaa cgtgcgccgc aacccgtccg gtgttgtcgc cgaagtcgag cagtggcgag
     4081 ccctgctggt gctggacaac ctcgagcagc tcgttggcgc cgacgaggtg gtcgacgtgc
     4141 tgctccgcga gaccacgtcg ctcgcactct tgaccacgtc ccggcagcca ctgcacgtag
     4201 cgggtgagta cgaccacccc gtgccggcac tcgacgtacc cgccgacgac tcgcgagcgg
     4261 ccgtgaacgc gtcggctgcc gcccagatgt tcaccgactg cgtacgccga gtgcagcccg
     4321 ccttcgagct gacggatctg aacgcgggcc cgattgccca gatctgccga cgactggacg
     4381 gactgccact tgcgctgcag ctggtcgcgg ctcgatccaa ggtcctcggt ccgaaggcga
     4441 tcgccgcgcg actggatcag gctctcgacg tcgtctccaa ccagcggtca gcgtcgcccc
     4501 ggcagcacac cctgcgagag acgatcgcgt ggtcctatcg gctgctcgac gaggagcaac
     4561 gtcggatcct ccgcgggttg ggcgtgtttg ccggcggagg cgatctggat gccgtggccg
     4621 cagtatgcgg ggagccgacc acgacccgct catctgacgg actgctcgag gcactgtcaa
     4681 gcctcgtcga tgcgagcctg gtcgatgtgg tcgaggcggc tgacggtgag ccgcggttca
     4741 ccctgctcga gaccatacgt gcattcgctc gggacgagat gcggggagcc ggtgaactgc
     4801 aggcgatcca ggaacgacac gcttcccatt tcgtcgacga ggcggaggcg ctcgccgcaa
     4861 ccatcaacgg cccgggcttc gtgtccgtcg tgggtcagtt cgccgtcgac tacgacaact
     4921 tcagggaagc gctcgcctgg gccaccaccc ctggctccag cacaagtcca ggacacgatg
     4981 ccaacgctct tggcctgcga ctcattgcgg ccctgatgcg gtactggcgg ctggcgggcc
     5041 tcaacgccga ggacaagtgg tggaccgagc gaatactcga ggtggccgcc gcagtcgaca
     5101 gtgcagcgta cggccgggtg ctcgaacgcc tcgccgtgca tcgcatgagc acgaatcctg
     5161 cggaagccaa ggaactggcg cgcaaggtgg tggagatcgg ccgccgggtg aacgacgaac
     5221 ggctcgttgc caactcgctc gtcacgctcg gggacattca catccaggtg gaaggcgatc
     5281 gagaaggcgg cattgctctg tacaaggagg cctggccgca tgcgcagcga tctgaggatg
     5341 cggacgccca agccaacgtt ctcgaagggc tatgcgaagt atcaacgctc tccggtgact
     5401 tcgagggggc gctcacgcag gtagcacgcg aggaagagct gattcgcgct cgggacaacc
     5461 ccctcgacag actcaggcac gagcacatgg tggcgtgggt ccaacggcgc gccggtcgtc
     5521 ctgagcaggc gctggactgg atacagcgag cggtccgctc tgcgcttgcc atcggcttcg
     5581 cctatgcgaa cggcatgacg tacgtggccg aggactacgc gtgcatcctg gccgacctcg
     5641 gacaggccgc ggatgctgcc cgtctcctcg gtgcagcgga tgcacgacgc gaacggatcg
     5701 acgcgccacg cgcaccgtac gtcgaggccg acatcgaacc atccatctcg aaggcgcgag
     5761 ccgtcctcac ggcgagggag tgggaagacg catacgggga gggtcggcag cagcagctgg
     5821 aggacgctct caccgttgct catcgacgga cggaggcgat gtgaaggctg tcgtgtacga
     5881 ccgctacggc tccccggaca tgttgcgggt ggaggacgtc ccgatgccgt cgtcagtggc
     5941 ggggcaggtg ctggtcaacg ttgcggcgac gtccatcaag ctgagcgacg agctgtcgga
     6001 gctcagggca ccgggaagtc ctgcggaggg ccttctccag tagccccgtc gacctgttcg
     6061 cggagcaggt cggcgtgacc agtgtggcgg gcgtactcct cgatcacgtc cgccagcaac
     6121 cggcgcaggc tcatcccgtt tgaagtcgtc tgctccagtc ctccgcaaga cagcacctcg
     6181 gccagcgcgt cgcgggagcg gtgcacagtg tcgacccaga gcgcacgcag cgcatcgggg
     6241 gtgtcgtcgg ccgccgtact ccaggcccag ccctcccagt ctctctcggg gtccagttgg
     6301 tcgaagggcg ccatggactg gcggccggcg agcctgttct ggaagtacag ctcctcgacc
     6361 cacgccaggt gcttgagcag gcctcccagc gtcaccgacg acgcaccgag ccgcaggccc
     6421 agtccctcgg cgccgacatc ggcacacttc caagcgaacg tgcgccggtt gcgttccagg
     6481 gtgatcacgg ccgtgtcgac ctcgtcgccg cgcatgaagc gctcgatatc ccacactggt
     6541 ggatggccgg gcgtgaagcc gtaggtgctc gtcatgcaac tcaccatatg catggttccg
     6601 gacgaaacac gtccgcaacc aactcgcggc tcggtcgacc ttcttggcac tcccgac
//
LOCUS       DATBFZ010000083         6712 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_2348481, whole genome shotgun sequence.
ACCESSION   DATBFZ010000083 DATBFZ010000000
VERSION     DATBFZ010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 6712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6712
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2348481"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1738)
                     /locus_tag="VH419_02505"
     CDS             complement(<1..1738)
                     /locus_tag="VH419_02505"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF020846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S53 family peptidase"
                     /protein_id="HEY7042519.1"
                     /translation="MTISVALAPAHSAALQRRLEAVYDRRSSRYHQWLSTGQFAHRYG
                     PSDRIRHAVTRYLRAHGLDVSTAASPFLIRATGSSDDVSAAFHTRLSSYRNARGVRYF
                     SNSTAVRLPPRLASHVNGVIGLSNTLRAHSSVKRTPFTGLKGARKSTVSCEAPYPTAQ
                     QLFDAVNNGVGFPFGYGGGPGCNGLTPSQDNGIYGAPHVGSRGKGAGADVAVFELSAY
                     QHSDIDTWTKQFYGASFHAPLVDVDVDGGPLNPQCPTGDTCPPQFEGYAGDIEVVADI
                     QMELAMAPAVRHLIVYNAPNDFTGQTELDEWTRIADDNTADTISSSWGICENDIDAGY
                     AQAENLLFEQMALQGQSVFGASGDTGSYDCIRADGSTILNVNDPPSQPWVTSVGGTSF
                     EHFNPGTTQHPNYPHAGEAPWNVDNLCNDSADEGGHPGFFWCGATGAGGGGSSQFWGR
                     PFYQRGPGVISSDTVYGNGSTHCSLARVGTPCRETPDISADADEYTPYAEYCTGSAAT
                     PFSVCATFSDQQTPPGWFGIGGTSLSSPLWAGIAVDRVSYTGHRFGNANPLLYLAYNA
                     APRRFFHDVTGAG"
     gene            2232..3506
                     /locus_tag="VH419_02510"
     CDS             2232..3506
                     /locus_tag="VH419_02510"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY7042520.1"
                     /translation="MTDARHSDARPRVLVLGGGFAGIGAARKLAKADVDVVIVDEHDY
                     HTFQPLLYQVATDLLPPITVGHPLRDLFHDQANARFHQAQVTSVDLGGRTVEFAHMDR
                     LGYDYLVLALGAGVNFFGVEGAAEHAFPMYTLADAVRLKEHILERWEMADKEPDVIED
                     GALNVVVVGGGPTGVESAGAIAELYRAVFTEDFPDIPQDQAQIVLVEAGPEIFAMFKP
                     GLRRYAEKALARRGVEVLTGEVVESIRPTRVSLRSGREIPAHTLVWGAGLKANPLVHS
                     LGVSLERGDRVPALPDLSLDGHPEVFAVGDAAWITDTKTGNVLPQLGSVALESGHHAG
                     ENIARLVEGAETKPFAYFDKGTMAAIGRGAAVMQGPHGRTMKGRTAWLAWGSVHLALL
                     STGEDRAKAVIDWTWAGATHERAARITVRTDE"
     gene            complement(3554..6223)
                     /locus_tag="VH419_02515"
     CDS             complement(3554..6223)
                     /locus_tag="VH419_02515"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEY7042521.1"
                     /translation="MTAQAQASTALLLESKLAVPEQRARPVTRARLHTLLDRAVQGPL
                     TLVSAAAGSGKTTLLSGWVTGRDSSLPRVSWLSLDKSDARPPAFWTGFVESLRRSGVE
                     VERAGLPVQLMAPDLDMLCGLAAALSSAPHPVVVVLDGCSVLADTVFEELDFLLQHSS
                     ARLRLVLLTREDPPLPLHRHRLEGTITELRQQDLAFTDSEVASLLSRSGVQMSARRVA
                     MLTDRTEGWAAAIRFAAVSLAAGGDADEIASVPVGTATPVAEFLTAEVMDTQPADLRD
                     ALLRSSVVDVMDPGLFDELTGRNDGHRVLDLLVRRNAMLLPVPGLARSYRYHELLRDL
                     LRHQLAYEQPELLPRLHQVAAGWLAEHGRVDEAVGHAAHAGAWSQAARHLVDGYAIGS
                     LLSGDEAASLGAVFANLPNDVIDAPTCVVRAAIALTAYDDVLAANELHRAEELCDHDP
                     PDQAVQLAVSVLGVVVAGRQSDVEKGLAAASTAEEILSGATQNNAQRAALTTLISTTK
                     AELHVWAGDVEAARADCTAGITAADGVGQDATLVARCLGQAALIEALQGHLRRAADLS
                     ARAHEIVTGSGNGGSKVSIALETARAWVATERLELEDARHHNVARTQSGETGAATAMI
                     VQLLTARLLRLDGDVDGCGRLLDELHASRVGSSLPAWLRARLAAEDVALINATQGRWS
                     GPPQVTADLETLPDLEAWLVRRSTGIDESTRPHEGSHLTALNHVRAPVDTRVTGWLVE
                     ADRCVADGQPARARTAVNRALLLAGREGMRRPFSEAPVRVRDFLMEDPALAEARRWVD
                     ASAGGGPGQSPAAIGMRDASPPAAVFVALTDKEQEVLGHLADQRTNDEIAAVMVLSVN
                     TIRTHVRSILRKLSVSRRQDAVRQARALNLIAS"
     gene            complement(6337..>6712)
                     /locus_tag="VH419_02520"
     CDS             complement(6337..>6712)
                     /locus_tag="VH419_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002528011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY7042522.1"
                     /translation="VSGWLFDHHDLTAYSQTFVWVVIFFFASAGASAAYLTVSETWPI
                     EIRSEAIAVFFAIAQIFGAIGPAFFGALIGDGSSRSGLVVGYVVGGVIMLLGGVVEIA
                     FGVDAEGASLEQVTKPLTSVAE"
BASE COUNT         1114 a         2165 c         2354 g         1079 t
ORIGIN      
        1 gtcccgctcc ggtcacgtcg tggaagaagc gacgtggagc ggcgttgtag gcgagataca
       61 acaacgggtt cgcgtttccg aaccggtgcc cggtgtagct gacccggtcg acggcgattc
      121 ctgcccacaa cggcgaggag aggctcgtcc cgccgatacc gaaccatccc ggcggcgtct
      181 gctggtcgct gaacgtcgcg cagacgctga agggcgtggc agcgctgccg gtgcagtact
      241 ccgcgtaggg cgtgtactcg tccgcgtcag cggagatgtc aggagtctcg cgacagggtg
      301 taccgaccct cgccagcgag cagtgcgtcg agccgttccc gtatacggtg tccgagctga
      361 tcacaccggg accgcgctgg tagaacggcc gtccccagaa ctggctggat ccgccacctc
      421 cggcgccggt cgccccgcac cagaagaagc ctgggtggcc gccttcgtcg gcgctgtcgt
      481 tgcagaggtt gtcgacgttc cacggcgcct caccggcatg cggatagttc gggtgctgcg
      541 tcgtaccggg gttgaagtgc tcgaacgacg taccgccgac gctggtgacc catggctgtg
      601 acggcgggtc gttgacgttg aggatggtcg aaccgtcggc gcggatgcag tcgtacgagc
      661 cggtgtcgcc agaggcgccg aagacgcttt ggccttgcag cgccatctgc tcgaagagga
      721 ggttctccgc ctgtgcgtag ccggcgtcga tgtcgttctc gcagatcccc cagctggagc
      781 tgatcgtgtc cgcggtgttg tcgtcagcga tccgtgtcca ctcgtcgagc tcggtctggc
      841 cggtgaagtc gttcggcgcg ttgtagacga tgaggtgacg cacggccggc gccatcgcga
      901 gctccatctg gatgtcggcg acgacctcga tgtcgcctgc gtagccctcg aactgcggcg
      961 ggcaggtgtc ccccgtcgga cactgcgggt tcaacggtcc accatcgacg tcgacgtcga
     1021 cgagcggcgc gtggaaggac gcgccgtaga actgtttcgt ccaggtgtcg atgtcggagt
     1081 gctgataggc cgagagctcg aacacggcaa cgtcggcgcc ggcacccttg ccgcgcgatc
     1141 cgacgtgagg ggcaccgtag atcccgttgt cctgagacgg cgtgaggccg ttgcagcccg
     1201 gtccgccgcc gtacccgaac gggaacccga cgccgttgtt cacggcgtcg aacagctgtt
     1261 gtgccgtggg atatggggcc tcacagctga ccgtgctctt gcgtgcaccc ttgaggccgg
     1321 tgaatggcgt ccgtttgacg gacgagtgcg cgcgaagggt gttggacagt ccgatgacac
     1381 cgttcacgtg ggacgcgagc ctggggggca gccgcacggc cgtggagttc gagaagtacc
     1441 tgacaccgcg cgcattgcga tagctgctca acctcgtgtg gaacgccgca gaaacgtcgt
     1501 cgctggagcc ggtcgcgcgg atgaggaacg gcgacgccgc cgtactcacg tcaagtccat
     1561 gggcacgcag gtatctcgtg accgcgtggc ggattcggtc cgagggtccg taccggtggg
     1621 cgaactggcc ggtcgacagc cactggtgat accggctgct gcgcctgtcg tacacggcct
     1681 cgagacggcg ctggagggct gcactgtgag caggtgccag ggcgaccgag atcgtcatgg
     1741 atcggctgct gtaggcgccg gtgcgtgtgt ccgtgctgcg tgatcggccg gccgcaagcg
     1801 acacgagtcg atgactcctg acatgtcccg ctccgactgc cgggacggag ccggccgaag
     1861 cggtcagggc agcggtggtc agagcgagac cggcgactgc ggtcgtggcg ataccggtac
     1921 gtcgtgcaag catgcaaacc cccttcgggt ggagagggtc ctgcgatggc aggccagccg
     1981 tctcgtgacg ctgcgaggtt gcctcgtgcg gcccgacggc ggttgtgcga gatcatcgat
     2041 caggacggcc agtagcgaga cttctcccgg cccctgacga ggtcaacacc cgtgggctaa
     2101 ggattggcta agaccggccg cacgtgccgg acggtcggcg cgactggtct cggcggacct
     2161 catccgcggc ggaggatgcc gcccacgcac agagttgctc ggctggagat gagcacagag
     2221 gaggtcacag gatgactgat gcgcgacaca gcgacgcccg tccccgggtc ctcgtactcg
     2281 gaggagggtt cgccggaatc ggcgcggccc gcaagcttgc caaggccgac gtcgatgtcg
     2341 tcatcgtcga cgagcacgac taccacacgt ttcagccgct cctgtaccag gtcgccaccg
     2401 acctgctgcc cccgatcacc gtcgggcatc cgctacggga cctgtttcat gaccaggcga
     2461 atgctcggtt ccatcaggca caggtcacct cggtggatct tggtggacgc actgtcgagt
     2521 tcgcgcacat ggaccggctc gggtacgact acctggtgct ggcgctcggc gccggcgtga
     2581 acttcttcgg cgtcgaggga gccgccgagc acgcctttcc gatgtacaca ctcgcggacg
     2641 cggtgcggct caaggagcac atcctggagc gctgggagat ggcggacaag gagccggacg
     2701 tgatcgagga cggagccttg aacgtggtgg tcgtcggcgg cgggccaacc ggtgtcgaga
     2761 gtgcgggcgc gatcgccgag ctctaccggg ccgtcttcac tgaggacttc cccgacatac
     2821 cgcaggacca ggcgcagatc gtcctcgtcg aagccggccc ggagatcttc gccatgttca
     2881 agcccgggct tcgacggtac gccgaaaagg ccctggcacg acgaggggtc gaggtgctca
     2941 ccggcgaagt cgtcgagtcg atcaggccca cgcgagtctc gctgcgctcc gggcgggaga
     3001 ttcccgcaca cacactggtc tggggtgcgg gactgaaggc gaacccactc gtccactccc
     3061 tgggggtctc actcgagcgg ggggaccgag ttccggcgct gccggacctc agtctcgacg
     3121 ggcaccccga agtgttcgcg gtcggtgatg ccgcgtggat cacggacacg aagacgggga
     3181 acgttcttcc acagctcggt tcagtcgctc tggagtccgg acaccatgcg ggggagaaca
     3241 tcgcgaggct cgtcgaaggg gccgagacca agccgttcgc ctacttcgac aagggcacga
     3301 tggcggctat cgggcgaggc gcggccgtga tgcagggacc ccacggtcgc acgatgaaag
     3361 ggcggactgc ctggcttgca tggggttcgg tccacctcgc gctgctgtcg accggtgagg
     3421 accgcgccaa ggcggtcatc gactggacgt gggcaggcgc gacgcacgag cgcgccgccc
     3481 ggatcaccgt ccggaccgac gagtgaccga cgagtgaccg agccccgtac acagggttag
     3541 cagcgtcagg agctcaactg gcgatcaggt tcagtgcgcg ggcctggcga actgcgtcct
     3601 ggcgtcggga gaccgacagt ttgcgcagga tgcttcgcac gtgggttcgg atcgtgttca
     3661 ccgagagcac cataacggcg gcgatctcgt cattcgtgcg ttgatcggca aggtggccga
     3721 gcacttcttg ttccttgtcg gtgagtgcca cgaacacggc agcgggcggt gacgcatcac
     3781 gcatcccgat ggctgccggc gactgacccg gtccgccgcc cgcggatgcg tcgacccagc
     3841 gccgcgcctc tgccagcgcc ggatcctcca taaggaaatc tcgcacgcgt actggggctt
     3901 ccgagaaagg ccggcgcatg ccctcgcggc cagccagaag caacgctcgg ttgacggccg
     3961 ttctcgcccg ggccggctgc ccgtcggcga cacatcggtc cgcctcgacc agccagcctg
     4021 tgacgcgcgt gtcgacagga gcacgaacgt ggttgagggc ggtgagatgg gaaccctcgt
     4081 gtggccgggt cgattcgtcg atgcccgtcg agcggcgtac cagccaggcc tccaggtcag
     4141 gcagcgtctc caggtccgcc gtgacctgtg gcggacccga ccatcgtcct tgggtcgcgt
     4201 tgatcagggc gacgtcctcg gcggcgagcc gggcgcgcag ccaggcaggc aacgatgacc
     4261 cgacgcggga cgcatgaagc tcgtcgagca gccgaccgca gccgtcgaca tcgccgtcca
     4321 agcgcagcag gcgagccgtg agcagttgga cgatcatggc cgtagctgcg ccggtctcgc
     4381 cggactgggt tctcgcgacg ttgtgatgcc gtgcgtcctc gagttcgagt cgctcggtcg
     4441 ccacccatgc gcgcgcggtc tccaatgcga tcgagacctt cgacccgccg tttccgctgc
     4501 cggtcacgat ctcgtgggct cgggcggaca ggtcggctgc acgccgaaga tgtccttgca
     4561 gtgcctcgat gagcgcagcc tgtcccaggc agcgagcgac gagggttgcg tcctgtccca
     4621 cgccgtcggc ggctgtgatg ccggcggtgc agtcggcgcg cgcagcctcg acgtctccgg
     4681 cccagacgtg cagctccgcc ttcgtcgtgc tgatgagcgt cgtcaacgca gcccgctgtg
     4741 cgttgttctg ggtggcgccg ctgaggatct cctcggccgt cgaggctgcc gccagccctt
     4801 tctcgacgtc ggactgtctg cccgcgacca cgacgcccag gaccgagacg gccagctgga
     4861 cggcctggtc gggtggatcg tggtcgcaga gttcttcggc ccggtggagc tcgttggccg
     4921 ccagcacgtc gtcgtacgcc gtcaaggcaa ttgccgctct gaccacgcac gtcggggcat
     4981 cgatgacgtc gttgggaagg tttgcaaaga cagcgccgag cgaagccgcc tcgtcgccgg
     5041 acaacaatga cccgattgcg taaccgtcca ccaggtggcg ggctgcctgt gaccaggcac
     5101 ccgcgtgcgc ggcgtgcccc acagcttcgt cgacacggcc gtgctccgcc agccagccgg
     5161 ccgcaacctg gtgcagccga gggagcaatt cgggttgttc gtacgccagc tggtggcgga
     5221 gcaggtcacg gagcaactcg tggtaccggt agctgcgggc caggcccggg accggcagca
     5281 gcatggcgtt cctgcggacg agcaggtcga ggactcggtg cccgtcgttg cgaccggtga
     5341 gctcgtcgaa caggccaggg tccatcacgt ccacgacgct gctgcgcagc agtgcatcgc
     5401 gtaggtcagc gggctgggtg tccatcacct ccgcggtcaa gaactccgca accggcgtgg
     5461 ccgtaccgac agggactgag gcgatctcgt cagcatcgcc gcccgctgcc agcgagacgg
     5521 ctgcaaacct gattgctgcg gcccagcctt cggtgcggtc ggtcaacatg gccacgcgtc
     5581 gcgccgacat ctgaacgccg gagcgagaga gcaacgacgc gacctcggag tcggtgaagg
     5641 cgagatcttg ctggcgtagt tccgtgatcg tgccctccaa ccgatggcga tggaggggca
     5701 gcggcgggtc ttcgcgagtg aggaggacaa gtcgtagtcg cgcggaactg tgctgcagga
     5761 ggaagtcgag ctcctcgaaa accgtgtcag ccaggacgct gcatccgtcg agcacgacca
     5821 caacaggatg gggcgcgctg gacagcgcgg cggcgagccc acacaacatg tcgaggtcgg
     5881 gcgccatcag ctggacgggc agacccgccc gctcgacctc gacaccactg cggcgcaggc
     5941 tctccacgaa gccggtccag aaggcaggcg gccgggcgtc gctcttgtcc agggacagcc
     6001 acgagacccg tggcagtgag gagtcccggc cggtgaccca gcccgacagc agggtggtct
     6061 tgccacttcc ggcggctgcg ctgaccaggg tcaacgggcc ctgcaccgca cgatcgagaa
     6121 gcgtatgcag ccgcgcccgg gtcaccggcc tcgcgcgctg ctccggcaca gccaacttgc
     6181 tctccagcag cagggcggta ctcgcttgcg cctgagcggt caccagatga ggagacgcag
     6241 cagctcggag ttgacggcga tcgccggccg tccccgatct tgacgagcgt cgagcccggg
     6301 ccggcggata cgactcttgg gaaagcagtg ccatggctac tccgcgaccg aggtgagggg
     6361 tttggtgacc tgttcgagcg acgcgccttc ggcatcgaca ccgaaagcga tctccacgac
     6421 gccgccgagg agcatgatca ctccgccgac gacatacccc acaacgagtc cgctgcgcga
     6481 cgatccgtca ccgatgagcg ctccgaagaa ggcgggaccg atcgccccga agatctgcgc
     6541 gatggcgaag aacacggcga tcgcctcgga ccggatctcg atcggccagg tctcgctgac
     6601 ggtgagatat gcggcgctcg cccccgccga ggcgaagaag aagatcacga cccagacgaa
     6661 ggtctgcgag taggcggtca ggtcgtggtg gtcgaagagc cagccgctga cc
//
LOCUS       DATBFZ010000084         3530 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_52744, whole genome shotgun sequence.
ACCESSION   DATBFZ010000084 DATBFZ010000000
VERSION     DATBFZ010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 3530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3530
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52744"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..701
                     /locus_tag="VH419_02525"
     CDS             <1..701
                     /locus_tag="VH419_02525"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEY7042523.1"
                     /translation="VDALIVTPTAKNRRHLARLLPGMVIVQVDRRVERLDADAILVDN
                     EAAGVGAVAHLIQAGHTNIGMLTGNVEVATAGQRLAGYKRALKDHGIAVRPALIRSGS
                     FHRDHAIDDATALIRARPAPTAIFAANNILAEGALVALGQLGLRVPRDMSLIAFDDEP
                     WMSMVEPAITTVRQPVVDIARSAAELALRRLREGREGRPSTVVFRTELVERASVAQLR
                     KVKAVKTTKAMSAR"
     gene            793..2313
                     /locus_tag="VH419_02530"
     CDS             793..2313
                     /locus_tag="VH419_02530"
                     /inference="COORDINATES: protein motif:HMM:NF012457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEY7042524.1"
                     /translation="MRLDLDLDFVREFAPDAFMFGVAYAPYCEGAGLNHPDGPKNTDW
                     TRRTSGRETDEGLGFWTNYADHVRLAASLGLNAFRLGIEWARCQPSASTEPTQAPSWD
                     EDALDHYADMIELVIDHGMQPVVTLHHFTHPLWLGLDIWLDDRGPDQLVEAQVEIVDR
                     INTRLIARGGKKAAHFLVYNEPNLVPLFYHQLGTYPVERTGSEYLLLAFDTMLAHYVK
                     AYDGIHDLFARRAWGAPHVGYTLASLCAYEYDKQLDDLLRLRAWGVDRRDVGPRLREC
                     RTRWRHRIDGLARSQLTDEQLERYERMVDRTAVELPPEGFNKTIDAVYSSPRARKLDY
                     LSLNVYEPFGAPRRDPGETERRVKWERYMMDGDVYRTFILAKNDFNDGLPIYMGENSC
                     ANLQAADGPATPRPDGWTRERYLKTYLMEMVRCMKEGVPVKGYLYWTLVDDVHPPRLG
                     LYNYDFVNGKILDTDGFGRPSGRIFAHLIAALRSGDRARIARAFVEAYPEQPPVLP"
     gene            complement(2347..>3530)
                     /locus_tag="VH419_02535"
     CDS             complement(2347..>3530)
                     /locus_tag="VH419_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042525.1"
                     /translation="RVMTEFIRDLRSHAVGSPEFAVSANVAYLGSLVVAFGSLWGALW
                     GRYVDFILMENHYRVEPRSPHLLLPRGTFAPWYRLGHAVTGAPTWICPSITVPRQLAG
                     ERRTTYYLLMFLEAYANAGRWGYYWWPGVDAETRLAATAPEALKTYIPFLDAHRELYE
                     DAVSDNQLAVLYADRSVLARPPTQDKYIALAQALAETGHQFDVVFCGDGQFNAESVDP
                     AALARYHTVVLPEARELGGDLVAALESYVHSGGRLVVFSESPLDPALIREVTGDLLVD
                     FWRHYRDEERTRIVDEVAAPPTAQIVASDPAARITRFRLGERQVLHLLAYSYDEVADA
                     VQPIRDLDLRIPWPTSEAPVCTLADLDGEHRLRGRLDNGALAVEIPILDPYAVLVVAP
                     S"
BASE COUNT          629 a         1138 c         1197 g          566 t
ORIGIN      
        1 gggtcgatgc gctgatcgtg acgcccactg ccaagaaccg ccgacacttg gcgcgcctcc
       61 tcccgggcat ggtcatcgtc caggtcgacc gccgcgtcga acgtctggac gcggacgcga
      121 tcctggtgga caacgaagca gccggcgtgg gtgcagtggc tcacctgatc caagccggcc
      181 acacgaacat cggcatgctg acgggaaatg tcgaggtggc caccgcagga cagcggctgg
      241 ccggctacaa gcgtgcgttg aaggaccacg gcatcgcggt gcgtccggcg ctgatccgct
      301 cggggtcgtt ccaccgggac catgccatcg acgacgccac ggcactgatc cgagcgaggc
      361 cggcgccgac ggcgatcttt gccgccaaca acatcctggc ggaaggagcg ctggtggcgc
      421 tcggacagct cgggctccgc gtcccgcgcg acatgtcgct catcgctttc gacgacgagc
      481 cgtggatgag catggtcgaa ccggcgatca ccacggtccg gcagccggtg gtcgacatcg
      541 ctcgcagtgc cgccgagctg gcactgcgtc ggcttcgcga gggccgagag ggtcgaccca
      601 gcacggtcgt cttccgcacc gaactggtgg agcgcgcctc ggtggcccag cttcggaagg
      661 tcaaggcggt caagaccacg aaggccatgt cggcacgatg aatgcgtaac gggtcggaac
      721 ctgaccttgg tcggcggcct catgataacg ttctcacatc gcgaccggcc acctcagctc
      781 aggagcagca cggtgcggtt ggacctcgac ctcgacttcg tcagggagtt cgcacccgat
      841 gcgttcatgt tcggtgtcgc ctacgcgccg tactgtgagg gcgcgggact gaaccatccc
      901 gacgggccga agaacaccga ctggactcgc cggacgtccg gtcgcgagac cgacgaaggg
      961 ctcggcttct ggacgaacta cgccgaccac gttcggctgg cggcatcgct ggggctgaac
     1021 gcgtttcggc tggggatcga gtgggctcgc tgtcagccgt cagcttccac agagccgact
     1081 caggcgcctt cttgggacga ggatgcgctc gaccactacg cggacatgat cgagctggtg
     1141 atcgatcacg gcatgcagcc ggtggtgacg ctgcaccact tcacgcatcc gttgtggctc
     1201 gggctggaca tctggctcga cgaccgcgga ccggaccagc tggtcgaggc gcaggtcgag
     1261 atcgtcgacc ggatcaatac gcgcctcatc gcgcgtggcg gcaagaaggc ggcgcacttc
     1321 ctggtctaca acgagccgaa cctggtcccg ctcttctacc accagctcgg cacctacccg
     1381 gtagagcgaa ccgggtcgga gtacttgctg ctcgcgttcg acacgatgct cgcgcactat
     1441 gtgaaggcgt atgacgggat ccacgacctg ttcgcgagac gggcgtgggg ggcgccgcac
     1501 gtgggataca cgctggcgag cctttgcgcc tacgagtacg acaagcagct ggacgacttg
     1561 ctgaggctgc gggcatgggg ggtggacagg agagacgtcg gcccccggtt gcgcgagtgc
     1621 cggacacgct ggcggcaccg catcgacggg cttgcccggt cgcagctgac cgacgaacag
     1681 ctcgagcgtt acgaacgcat ggtcgatcgg acagcggtcg agctgccgcc tgagggcttc
     1741 aacaagacga tcgatgcggt ctactcgtcc cctcgggcgc gcaagttgga ctacctctca
     1801 ctgaatgtct acgagccctt cggcgcaccg cggcgcgacc ccggcgagac cgagcgccgc
     1861 gtcaagtggg agcgctacat gatggacggc gatgtctacc gcacgttcat cctcgcgaag
     1921 aacgacttca atgacggtct gcccatctac atgggtgaga actcgtgcgc gaacctgcag
     1981 gctgccgacg gacctgcgac acctcggccg gacggctgga cccgagagcg ctacctgaag
     2041 acctatctga tggagatggt ccgctgcatg aaggaaggtg tgccggtcaa ggggtatctg
     2101 tactggaccc tggtcgacga cgtccatccg ccccggctcg gcctctacaa ctacgacttc
     2161 gtcaacggga agatcctcga cactgacgga ttcggtcggc catcaggccg catcttcgcc
     2221 catctgatcg ccgcgcttcg tagcggcgac agggcgcgga tcgcccgtgc gttcgtcgag
     2281 gcatatccgg aacaaccgcc ggtcttgccc tgagtctgca ggccatcgac cggtccgtcg
     2341 ggtcgcctac gacggcgcca ccacaaggac ggcgtacggg tcgaggatgg gaatctcgac
     2401 cgccagcgcg ccgttgtcca atcgcccacg gaggcgatgc tcgccgtcga gatcggcgag
     2461 tgtgcacacg ggagcttcgc tggtcggcca aggaatccgc aggtcgaggt cgcgaatcgg
     2521 ttgcaccgcg tcggccacct cgtcgtagga gtaggcgaga aggtgcagca cctgccgttc
     2581 gccgagacgg aacctggtga tccttgctgc tggatcggag gccacgatct gggccgtggg
     2641 aggtgccgcg acctcgtcga cgatgcgggt gcgctcctcg tcgcggtagt gccgccagaa
     2701 gtcgacgaga aggtcgccgg tcacttctcg aatgagcgcc ggatcgagcg ggctctccga
     2761 gaagaccacc agtctcccac cgctgtgaac gtacgactcg agtgctgcga cgagatcgcc
     2821 gccgagctct cgagcctcgg ggaggacgac ggtgtggtag cgcgccagcg cggccgggtc
     2881 gacgctctca gcgttgaact gtccgtcacc gcagaacacc acgtcgaact ggtgcccggt
     2941 ctctgcgagc gcctgcgcca acgcgatgta cttgtcctgc gtcggtggtc tcgccaggac
     3001 cgagcgatcc gcgtagagga cggcgagctg gttgtccgag acggcatcct cgtacagctc
     3061 gcgatgggcg tcgaggaacg ggatgtacgt cttcagcgcc tcgggagcgg tcgcggcaag
     3121 gcgggtctcg gcgtcgaccc ccggccacca gtagtagccc cagcgcccag cgttggcgta
     3181 cgcctcgagg aacatcagca agtagtacgt cgtgcgccgc tcgccggcca gctgccgcgg
     3241 aacggtgatg cttgggcaga tccaggtcgg agccccggtc actgcatggc cgaggcgata
     3301 ccacggggcg aacgtcccgc gcgggagcaa cagatgtggc gagcggggtt cgacccggta
     3361 gtggttctcc atcaggatga agtcgacgta ccggccccac agagcacccc acagagaccc
     3421 gaaagcgact accaagctcc cgaggtaggc gacgttggcg ctgacggcga actctgggct
     3481 gccgacagcg tgcgaccgca ggtcgcggat gaactcggtc atcacccgaa
//
LOCUS       DATBFZ010000085         6004 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_5606039, whole genome shotgun sequence.
ACCESSION   DATBFZ010000085 DATBFZ010000000
VERSION     DATBFZ010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 6004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6004
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5606039"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            491..1153
                     /locus_tag="VH419_02540"
     CDS             491..1153
                     /locus_tag="VH419_02540"
                     /inference="COORDINATES: protein motif:HMM:NF041646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VC0807 family protein"
                     /protein_id="HEY7042526.1"
                     /translation="MESNEAPAPVVSRRAGVLRTVALDVVGPLVVYRLCRSIGMAEVW
                     SLVIAGMLPGVGVLVDWLRWRTLEMVGVVVLGGIGLSIVLAMVSGNPKAVLLEGAAIT
                     AAFGVACLLSLGGRRPLIFYFGQAFYGGRHSHEGIELDIDYEQYKEARFFWRVVTVVW
                     GVTHIALAAALTMIVLGSSTGTALTFNRTVPWTLNGLLLVWSIWWGERLRAQKPDPEA
                     HD"
     gene            complement(1161..2138)
                     /locus_tag="VH419_02545"
     CDS             complement(1161..2138)
                     /locus_tag="VH419_02545"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEY7042527.1"
                     /translation="MASTPTAQPTEFARASEEVAAAFALGGVRRPLVLAATGEMGRIF
                     RLETDRGIFAVKELVERRTEEEVWQDVEFQERAGAVATTYLVARNVRTVDGRVLAEIS
                     GRQVRVQSWLDMAAPDPMLDPGLIGRMLAELHAAGEPRTDPVHPWYTTAVGAVRWSEY
                     ADRLTAARAPVASRVAAMVPRLVELEELLIAPTDLRTCHRDLWADNVRAMPDGTVCVF
                     DWDNCGPADPDQELAMLAFEFGMDSRDRIRTLHDAYVAAGGPGRVTQPGDLTMVIAQF
                     GHFVESAVQQFLDDEATPDDEEWALARLDELEERPLTIEAVQQIVDACA"
     gene            complement(2242..3090)
                     /locus_tag="VH419_02550"
     CDS             complement(2242..3090)
                     /locus_tag="VH419_02550"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY7042528.1"
                     /translation="MEYGFVAPFADARDLSEYAALAESHGWDGVFVAESVWGVDAWVA
                     LAAAAMTTQRLRLGTLLTPVSRWKPWDLASRVGTVDRLSGGRAIMSTGLGALHDGWLA
                     FEADEGRRQRAEKVDECLAVYDGLMRGQPFSFEGAHYTAHPTTFYPPDPPVQRPRPPV
                     WVVGAHMVGRPRQPSLERAARWDGLLPQVVHDEQRGKPQMDDFVDVVARVRRMRADAG
                     LPWDGYDVIVEGDSTGDFVQLDPASPQAWEQAGATWWIECWWSLEPGPEGAAELRRRL
                     AAGPPS"
     gene            3134..4618
                     /locus_tag="VH419_02555"
     CDS             3134..4618
                     /locus_tag="VH419_02555"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012923233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY7042529.1"
                     /translation="MSETRAARPVGDVLDAVVIGTGFAGIGMAIKLKESGRENFVVLE
                     KTDDVGGTWRDNTYPGCTCDVPSPLYSFSFAQNPHWTRFFSSQQEIWDYLRRLVVEYD
                     VAAHIRYGAEVTAATFDDAAGEWDVVVNGGETLRSRALVTGVGALHEPHVPDIPGLES
                     FTGTTFHSARWRHDHDLPGRRVAVVGTGASAIQFVPQIQPQVEHLDLFQRTPAWITPR
                     PDRAIDPKERGFYQRHPRIQRAVRNGVYWMMEARGTGFALTPKAMGFLEKSARKHLVR
                     QVPDPLLRAKLTPDYQIGCKRILLSNDFYPALGRPNVDVVTEKIREVRARSIMTVDGV
                     ERTVDTIIFGTGFNVIGNIPRMKIVGRHGAELSDVWGEHGMGAHLGITMAGFPNLFLL
                     LGPNTGLGHSSVVFMIESQIRYVVQALDLLEKRKATYLEVRPETEQHFLNRVQHRLAN
                     SVWQSGCSSWYQDDNGRNVAIWPHYSWQYWLATRRLKTSDFTLT"
     gene            4744..5472
                     /locus_tag="VH419_02560"
     CDS             4744..5472
                     /locus_tag="VH419_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003857431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEY7042530.1"
                     /translation="MVSLTGVNKWFGDLHVLKDIDLAIDRGEVVVVIGPSGSGKSTLC
                     RVINRLEPINEGEITLDGVPLPEEGKGLARLRAEVGMVFQSFNLFAHKTVLENVTLGP
                     VKVRNKSSSEAKELATKLLDRVGVGQQASKYPAQLSGGQQQRVAIARALAMQPKVMLF
                     DEPTSALDPEMIKEVLDVMVELAEDGMTMVVVTHEMGFARRAANRVVFMADGAIVEES
                     TPDEFFAHPKSDRAKDFLGKVLQH"
     gene            5601..>6004
                     /locus_tag="VH419_02565"
     CDS             5601..>6004
                     /locus_tag="VH419_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEY7042531.1"
                     /translation="MNCTKRTRFAALGAAALLAATLTACGDDSESGGSTDVDVSDNPT
                     FEAGTTMEKLADAGKITIGVKIDQPGIGNLEPGADAPEGFDIEIGRILAGALGIDDDS
                     INWKETISDNRESFIQGGDVDLVIASYSITDDR"
BASE COUNT         1033 a         2030 c         1979 g          962 t
ORIGIN      
        1 ctcgtcgtga gcggtgtccg ggtggatgag ctgcaaggca gaggagggag gcgatgcgca
       61 gcatcgttga ccgacgataa cgccgcagat cgccgcccgg gcggcgcgca acagggcgaa
      121 tgttggttga cggggtacta gacccagcac ggttcggcac ctgatgcgca gcgcgcgcac
      181 atcattctga gtggccgcat cctgagtggc tgccagcctg ctggcggccc ggaacgggca
      241 cagctcagga agtcggggcg agtgtccgtg gcctgtgttg aagtagcgcc atgagccaac
      301 agggctggca ggagtctctg cgggcagcgg atctcggcga ctgggtggtc ttgcacggcg
      361 gcgcggccgc tgtctatgtc gtgggctcgt tcagggccgc ggcttccgaa tcgtcggttg
      421 ggcgaaccgt ctgccgatga tgccgttcca cctgacgctt ggcgccatcc gccgtgcaga
      481 cttgacgatc gtggaaagca acgaggcacc ggccccggtc gtcagccgcc gggcaggagt
      541 cctgcggacc gtggcgctgg acgtggtcgg cccgctcgtc gtgtaccgcc tctgccgcag
      601 catcggcatg gccgaggtgt ggtcgctggt gatcgccggg atgctgcctg gcgtcggcgt
      661 actcgtcgac tggcttcgct ggcgcaccct cgagatggtc ggtgttgtcg tcctcggtgg
      721 gatcgggctc agcatcgtgc tggcgatggt gagcggcaac cccaaggccg tgttgctcga
      781 aggtgcagcg atcacggcgg cgttcggagt ggcgtgcctg ctgtcgctcg gcggacgccg
      841 accgctcatc ttctacttcg gtcaggcctt ctatggcgga aggcactcgc acgaaggcat
      901 cgagctggac atcgactacg agcagtacaa ggaggccagg ttcttctggc gcgtcgtgac
      961 ggtggtgtgg ggcgtgaccc atatcgcgct tgccgcggcg ctgacgatga tcgtgctcgg
     1021 atcctcgacc ggaacggcgc tcacgttcaa ccggaccgtg ccgtggacgc tgaacgggct
     1081 gctgctggtg tggtcgatct ggtggggtga acggctgcga gcccagaaac ccgacccgga
     1141 agcccacgac tagactacgg tcacgcgcag gcgtcaacga tctgctggac ggcttcgatc
     1201 gtgagcggac gctcctccag ctcgtccaac cgtgcgagcg cccattcctc atcgtccggg
     1261 gttgcctcgt cgtcgaggaa ctgctggacc gccgactcga cgaagtggcc gaactgagcg
     1321 atcaccatcg tgaggtctcc cggttgggtg acccggccgg gcccgccggc cgcgacatag
     1381 gcgtcgtgca acgtccggat ccggtccctc gagtccatcc cgaactcgaa ggcgagcatc
     1441 gcgagctcct gatcgggatc ggccggaccg cagttatccc agtcgaagac gcacaccgtg
     1501 ccgtcgggca ttgctcggac attgtcggcc cacagatcgc gatgacaggt tcgcaggtcc
     1561 gtcggcgcaa tcagcagctc ttccagctcg acgagacgcg gcaccatcgc ggcgaccctc
     1621 gacgcaaccg gcgcacgagc cgccgtcaga cgatcggcgt actccgacca tcgaacggca
     1681 cccaccgcgg tcgtgtacca gggatggacc ggatccgtgc gcggttcgcc cgcggcatgc
     1741 agctcggcga gcatccggcc gatcaacccc gggtcgagca tcggatcagg ggccgccatg
     1801 tcgagccagc tctgcacccg cacctgtcga cctgaaatct ctgccaacac cctcccgtcg
     1861 acagttcgca cgttgcgggc caccagatag gtcgtggcga ctgcgcccgc tcgctcctgg
     1921 aattccacgt cctgccagac ctcttcctcg gtccggcgct ccaccagctc cttgacggca
     1981 aagatccctc gatcggtctc caggcggaag atgcgaccca tctcgccggt cgccgcgagc
     2041 accaacggac gacggacgcc ccctaacgcg aaggcggccg ccacctcttc ggacgcgcgg
     2101 gcgaactcgg tcggctgtgc cgtcggcgtg ctggccatgc gctcatcctc ctgcgcgagc
     2161 cggggagcag acccggacca cgccgagccg tgacggcttg atgtcgccgc atgaccgacg
     2221 tgagcagttc gcgcctgaca ctcaacttgg cggtccggcg gccaggcgtc tcctgagctc
     2281 ggccgcaccc tcgggtcccg gctccaggct ccaccagcac tcgatccacc aggtcgcacc
     2341 agcctgctcc caggcctgcg gcgatgccgg gtcgagctgg acgaaatcgc cggtgctgtc
     2401 accctcgacg atgacgtcgt agccgtccca tggcagtccg gcgtccgcgc gcatccggcg
     2461 tacgcgggcg acgacgtcga cgaagtcatc catctgcggc ttcccgcgct gctcgtcgtg
     2521 gaccacttgc ggcagcaggc cgtcccaacg cgcggcgcgc tccaacgacg gctgccgggg
     2581 ccgccccacc atgtgcgccc cgaccaccca gaccggaggg cggggacgct gcaccggcgg
     2641 gtccggtggg tagaacgtcg tgggatgagc ggtgtagtgc gcgccctcga agctgaacgg
     2701 ctggccgcgc atcagaccgt cgtacacggc caggcattcg tccaccttct cagcgcgctg
     2761 tcgacgccct tcgtcggcct cgaaggccaa ccacccgtcg tgaagcgccc cgagcccggt
     2821 gctcatgatc gcgcgtccgc cactcagacg gtcgacggtc cccacccggg aagcgagatc
     2881 ccacggcttc catcgcgaga ccggtgtcag cagcgtgccg aggcggaggc gctgcgttgt
     2941 catcgcggct gccgcgagcg ccacccacgc atcgacaccc cacaccgact cggcgacgaa
     3001 gacgccgtcc catccgtggg actccgccag cgcggcgtac tccgagaggt cacgtgcgtc
     3061 cgcgaacgga gccacgaagc cgtactccat agcagccaat ctaaggcggc gtgcccggtg
     3121 ttaccttcgt cgcgtgagcg agacccgcgc cgcgcgccca gtcggggacg tgctcgacgc
     3181 cgtcgtgatc gggaccgggt tcgccggcat cgggatggcg atcaagctca aggagtcagg
     3241 ccgcgagaac ttcgtcgtcc tcgagaagac cgacgacgtc gggggtacct ggcgcgacaa
     3301 cacgtacccg gggtgcacgt gtgacgtccc ctcgccgctc tactcgttct ccttcgccca
     3361 gaatccccac tggacgcggt tcttctcgtc gcaacaggag atctgggact acctgcgacg
     3421 attggtcgtc gagtacgacg tcgccgccca catccggtac ggcgctgagg tcacggcggc
     3481 gacgttcgac gacgccgccg gcgaatggga cgtcgtcgtg aacggtggtg agaccctgcg
     3541 cagtcgggct ctcgtcaccg gcgtcggcgc gttgcacgag ccgcatgtcc ccgacattcc
     3601 tggcctcgaa tcgttcacgg gcaccacctt ccactccgcg cgctggcggc acgaccacga
     3661 cctgcccggt cgccgggtgg ccgtcgtcgg gaccggcgcc agcgcaatcc agttcgtgcc
     3721 gcagatccag ccacaggtcg aacacctcga cctgttccag cgcacgccgg cgtggatcac
     3781 gccgagacca gatcgtgcca tcgatccgaa ggagcgcggt ttctaccagc gccatccgcg
     3841 gatccagcgg gcggtccgga acggtgtcta ctggatgatg gaggctcgtg gcaccggttt
     3901 cgcgttgacc cccaaggcca tggggttcct cgagaagtcg gcgcgcaagc acctggtgcg
     3961 gcaggttcca gaccccttgc tgcgagccaa gctgaccccg gactaccaga tcggctgcaa
     4021 gcggatcctc ctctcgaacg acttctaccc tgcgctgggc cggccgaatg tcgacgtcgt
     4081 cacggagaag atccgcgagg tcagagcgcg cagcatcatg accgtcgacg gggtcgagcg
     4141 caccgtggac acgatcatct tcgggaccgg cttcaacgtc atcgggaaca tcccgcggat
     4201 gaagatcgtt ggccggcacg gtgcggagct cagcgacgtc tggggcgagc acggcatggg
     4261 tgcccacctg ggaatcacga tggcaggctt cccgaacctg ttcctgctgc tcgggccgaa
     4321 caccggtctc ggccacagct ccgtggtgtt catgatcgag tcccagatcc ggtacgtcgt
     4381 gcaggctctg gaccttctcg agaagcggaa agcgacgtac ctcgaggtgc gaccggagac
     4441 cgagcagcac ttcctgaatc gggtgcagca ccggctcgcc aacagcgtct ggcagtctgg
     4501 ttgctcgagc tggtaccagg acgacaacgg ccgtaacgtc gcgatctggc cgcactacag
     4561 ctggcagtac tggctcgcga cccgacgcct caagacctcc gacttcaccc tgacctgagc
     4621 ggttcttcgt tgacacgcgg ggcaaccact ccgctagggt caggcatggc tgacgcaccg
     4681 cccgccatgt ccgacccgag cagttctcag tcccccacgc cccaacagag cacgtcgcag
     4741 cccttggtgt cgctcaccgg cgtcaacaag tggttcggcg acctgcacgt gctcaaggac
     4801 atcgacctcg ccatcgaccg cggcgaggtc gtcgtggtca tcgggccgtc cggctctggc
     4861 aagtcgacgc tttgtcgggt gatcaaccgg ctcgagccga tcaacgaggg cgagatcacc
     4921 ttggacggcg taccactgcc cgaggaggga aaggggcttg cacggctgcg cgcggaggtg
     4981 ggcatggtct tccagtcgtt caacctgttc gcgcacaaga ccgtgctcga gaacgtcacg
     5041 ctcgggccgg tgaaggtccg gaacaagagc agcagcgagg cgaaggagct tgccacgaag
     5101 ctgctcgacc gcgtcggagt cggccagcag gcgagcaagt atccggccca gctctcgggc
     5161 ggtcagcaac aacgcgtcgc cattgcccgg gcgttggcga tgcagcccaa ggtcatgctc
     5221 ttcgacgagc cgacgtcggc gctcgacccg gagatgatca aggaggttct cgacgtgatg
     5281 gtcgagttgg ccgaggacgg catgacgatg gtggtcgtca cgcacgagat ggggttcgca
     5341 cgccgagccg cgaaccgcgt cgtcttcatg gccgatggtg cgatcgtcga ggagtcgacg
     5401 ccggacgagt tcttcgccca tcccaagtcc gaccgggcga aggacttcct cggcaaggtt
     5461 ctccagcact gagacgacag gcgaaaggag cgcacccagc acggtcgaat tcgatccgga
     5521 caacccgact ggacgaatca ggagacgtag ccatcgccgg aaagaccggc gcgccgaaca
     5581 cttcaagggg aatcagcacc atgaactgca ccaaacgcac tcgtttcgca gctcttgggg
     5641 ccgctgcact gctcgcggcc acactcaccg cctgcgggga cgactccgag tcaggcggca
     5701 gcaccgacgt cgacgtgtcg gacaacccga cgttcgaggc cgggaccacg atggagaagc
     5761 tggccgatgc cggcaagatc accatcgggg tgaagatcga ccagcccggt atcgggaacc
     5821 tcgagcccgg cgcagatgca ccggaggggt tcgacatcga gatcggccgg attctcgccg
     5881 gcgcgctcgg aatcgacgac gacagcatca actggaagga gacgatctcc gacaacaggg
     5941 agtcgttcat ccaaggtggt gacgtcgacc tcgtcatcgc gtcgtactcg atcaccgacg
     6001 accg
//
LOCUS       DATBFZ010000086         8311 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_1364821, whole genome shotgun sequence.
ACCESSION   DATBFZ010000086 DATBFZ010000000
VERSION     DATBFZ010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 8311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8311
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1364821"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..951)
                     /locus_tag="VH419_02570"
     CDS             complement(<1..951)
                     /locus_tag="VH419_02570"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012921885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HEY7042532.1"
                     /translation="MTARHLPRWSELQPLLRPKPVRLSPTERRLSTAASISDLRRMAK
                     RRAPRAVFDYTDGAAGDEISLRRSRQVYAQLEFSPSVLRDVSAIDTSTTLLGKSAALP
                     LVFAPTGFTRMMHTQGEPAVGRVAARTGIPYALSTMGTTSVERLASEVPDVRRWFQLY
                     VWRDREASSDLVARAKEAGYDALILTVDVPVPGPRLRDVRNGLTIPPALSLRTVAEGA
                     LHIGWWFDLLTTEALEFASLRDSEGTVAELIARMFDPATTMDDIDWLRSTWDGPLIVK
                     GIQTAADACRVVDAGADALIVSNHGGRQLDRAPTPLEVLPS"
     gene            1150..1461
                     /locus_tag="VH419_02575"
     CDS             1150..1461
                     /locus_tag="VH419_02575"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEY7042533.1"
                     /translation="MIIRVFRPTIRAGKEEEFESFLRDTAIPLVSRQPGLVAQHAGRP
                     RDASSTEYLYVTVWQDIESIRAFAGERWEEAVITPDEEHMLEATWIGHYEVIDTPAPS
                     R"
     gene            complement(1475..3424)
                     /locus_tag="VH419_02580"
     CDS             complement(1475..3424)
                     /locus_tag="VH419_02580"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEY7042534.1"
                     /translation="MTETFEQIEQWVASYQRASRETTDGLADISQAAPHPSRNLVACT
                     LGVRDSPDADVRRQVVLVDSPGSTRPVLDTDETSAWPVWSPDGTRLCVLAGRDGEHTT
                     AVVIDVEGTSKTTRAEDLGGSVEIARWSREGKRLALIVADAGAEISDVWGSGKVSGGV
                     TESWRPQVFPHEGGRRRLVVWEPASGSHCTITALNVWEADWYGDDVVALVSEGAGEDA
                     WYSARLVRIRLDGEVSPLYEATYQLAHPCGSPDGSSWSVLTGFASDRDLLAGTLIVAR
                     EGAEPVVMETADTHVTDQRWVDDDRLLFIGHRGLDTVVGTVDVGSARCVELWSGQATT
                     GLYQPDLGGLTADGRVVLLLEQHDMPPRLVRVGADGDEVLLDTRGPGTEHVVHTTGQT
                     RALRWTSTDGLDIEGLLTTPDGDAPYPIVLHVHGGPVGAFQDGWIGRDAHTTILVARG
                     YAVFRPNPRGSAGRGAEFAERVLGDMGGMDAQDLLTGLDHLVAEGVADPGRIGVTGQS
                     YGGFMAAWLPCLTDRFAASVSRSPCTDWRSQHLTSNIAEFDRIFLDGEPFDPESQYQT
                     RSPLTHHELCTTPMLLTAGSRDLATPASQAQQMYKALAERGVEAALAIYPEEGHGVQA
                     LPALADQCARMVAWFERFMPSGSGR"
     gene            complement(3514..4662)
                     /locus_tag="VH419_02585"
     CDS             complement(3514..4662)
                     /locus_tag="VH419_02585"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY7042535.1"
                     /translation="MTDLLDAPATEEHAQQQYELVDCDVHPIMKGGLADLRPFLSEAA
                     KRRLGLDGRRNLTTVGHREAVSIPRNQLYMNQAGVLRDDARAPDGSAPGADPAFTAHQ
                     LLDANGISRAVLIGGEVLGLGAMPDPDAAALIASAYNDWLAATWLAADDRYRGTIVVG
                     AQDPELAAREIRRAGEDQRFVAVLLPLTNILMGQRHYYPIYEAAAELRLPITVHPNSG
                     EGIFHTSPPLAGGTPTYYVEWHTGLSQVFQANLISLVCHGVFERFPDLKVVLTEGGLG
                     WIPDVLWRLDKNVKGLRDEVPWVRRLPSEYVFDHVRFTTQPLPEPKRLAHLHMLCEIA
                     CAERTLMFSSDYPHWDFDDPRYALTTLPAQIRQRVMVDNALETYGPRL"
     gene            4766..5116
                     /locus_tag="VH419_02590"
     CDS             4766..5116
                     /locus_tag="VH419_02590"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HEY7042536.1"
                     /translation="MEHRVGSVDDVRRAGCRIVQVDGRRVGVISVDAGFYAVHDRCPH
                     MGASMCAGSVGGTLVASEPHELVYGMEDRVIRCPWHGWEFDLETGRSLLEPERIGLKT
                     YRVTNDGHDVILHT"
     gene            complement(5157..5804)
                     /locus_tag="VH419_02595"
     CDS             complement(5157..5804)
                     /locus_tag="VH419_02595"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY7042537.1"
                     /translation="MGGCCEPRGYGDLFTERYSRHLAKRYRKRGLDKTARRIVAFLTD
                     RGIDGATVLELGGGVGDIQLELLRRGAARATNLELVDSYDEDARALAAEAGLADRITR
                     RQVDLALTPDLVEPHDIVVLHRVVCCYRDHQRLLAAAADHATRLLVFSHPPRNLVSRL
                     VFATENLLFRLRRTAFRTYLHDPVALTAAAERGSLETRYLSNALTWHIVGLERPT"
     gene            complement(5834..6148)
                     /locus_tag="VH419_02600"
     CDS             complement(5834..6148)
                     /locus_tag="VH419_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003849969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family transporter"
                     /protein_id="HEY7042538.1"
                     /translation="MRWVLLSVAIVAEVSGTLSLRLAAAGAKIWYGVVVTGYLLAFTF
                     LSLALAEGLGLGVAYGTWAAAGVALTAVASRFLFGEHLGVLMGCGIACIAAGVLLVEV
                     GH"
     gene            complement(6148..6504)
                     /locus_tag="VH419_02605"
     CDS             complement(6148..6504)
                     /locus_tag="VH419_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020576019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HEY7042539.1"
                     /translation="MIWLLLAAAILSEVTATLSLKAALNHHLLYVVVAVGYCASFVLL
                     ALVLRRGMALGVAYGIWSAVGVAATALLSALIFDEALTTVMVIGLGLVVCGMLLVELG
                     SHPSRPGPPTRDGRRT"
     gene            6881..7594
                     /locus_tag="VH419_02610"
     CDS             6881..7594
                     /locus_tag="VH419_02610"
                     /inference="COORDINATES: protein motif:HMM:NF021496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2218 domain-containing protein"
                     /protein_id="HEY7042540.1"
                     /translation="MRAAVLELLTEQPMHGYQLMHAIADRTGGRWTPSPGAIYPTISQ
                     LEDEGLVVIAAESGRKLVTLTDEGRALAQADRDAGVDPFTRFEPASPGLDLRRLSDEL
                     RNAVRDVARHGDSTMRSATAAILADARKRVYLLLAEGSAESDASYVARASVATAKPER
                     YVKQLAAHFGRRSEVRAEPSGTRVILTDGECLLRTGDDSIELVVSADDPDRLESVKQV
                     VGSHLERFGQRDGLTVSWT"
BASE COUNT         1409 a         2796 c         2823 g         1283 t
ORIGIN      
        1 ggatggcagc acctccagcg gcgtcggtgc ccggtcgagc tgccggccgc catggttcga
       61 gacgatcagc gcgtccgcgc cggcatcgac gacacggcac gcatccgccg cggtctggat
      121 gcccttgacg atcaacggcc cgtcccaggt cgaccgcagc cagtcgatgt cgtccatcgt
      181 cgtggccggg tcgaacatcc gcgcgatgag ctccgcgacg gtgccctcgg agtcgcggag
      241 tgaggcgaat tccagtgcct cggtggtgag caggtcgaac caccatccga tgtgcagcgc
      301 accttccgcg acggtccgaa gcgacagcgc cggcgggatc gtcagaccgt tccgaacgtc
      361 gcgcaaccgc ggtccgggaa ccggtacgtc gacagtgagg atgagggcgt cgtacccagc
      421 ctccttggcc ctggccacca ggtcgctgct cgcctctcgg tcgcgccaca cgtagagctg
      481 gaaccagcga cgcacgtcgg ggacctcgga tgcgagccgc tcgacggagg tcgtgcccat
      541 cgtggacaag gcgtacggga tgccggtgcg cgcagcgact ctgccgacgg caggctcgcc
      601 ctgggtgtgc atcatgcggg tgaatcccgt cggcgcgaac accaacggca aggcggccga
      661 cttcccgagc agagtggtcg acgtgtcgat ggcggacacg tcgcgcaaca cgctggggct
      721 gaactcgagc tgggcgtaca cctgtcggga gcgccgcagg ctgatctcgt caccagcggc
      781 cccgtcggtg tagtcgaaca cggcacgcgg cgcccgacgc ttggccatcc gacgcaggtc
      841 gctgatggag gcggcggtcg acaagcgtcg ttcggttgga ctgagccgga ccggcttcgg
      901 acggagcagc ggttggagct cggaccagcg cggcaagtgt ctcgcggtca ccctcgtgtt
      961 ctacacgacc cgcacccggc gggtcgagcc ccgtcgacag gctcaggaac cacctgtcga
     1021 gctcattgga acccgtgatc tgggcaggct gtatcagtgg gttcggacgg tgccggcagg
     1081 gcttggccgg acctgatccg gcgggatcgg tcgtagcctg gtggcccaac gatcaggatg
     1141 gagccagcca tgatcatccg cgtcttcagg ccgaccatcc gtgccggcaa ggaagaggag
     1201 ttcgagtcgt tcctccgtga cactgcgatc cccctcgtgt cacggcaacc agggctcgtc
     1261 gcgcagcacg ctgggagacc acgtgatgcg tcctccacgg agtatctgta cgtcacggtt
     1321 tggcaggaca tcgagtcgat ccgggcattc gcgggcgaac gctgggaaga ggccgtgatc
     1381 acgcctgacg aggagcacat gctcgaggcg acctggatcg ggcactacga ggtgatcgat
     1441 acgccggcgc cctcccgtta atgcgccgct gccgtcaccg gccggacccg gagggcatga
     1501 agcgctcgaa ccaggcgacc atccgcgcgc actggtctgc gagcgccggc agcgcctgta
     1561 cgccgtgccc ctcctcggga tagatggcga gcgctgcttc cacaccgcgc tcggccagcg
     1621 ccttgtacat ctgctgcgcc tggctcgccg gtgtcgcgag gtcgcgggag ccggcggtca
     1681 gcaacatcgg cgtcgtgcac agctcgtggt gggtgagcgg gctgcgggtc tggtactgcg
     1741 actccgggtc gaacggctca ccgtcgagga agatgcggtc gaactccgcg atgttgctgg
     1801 tgaggtgctg cgaacgccag tcggtgcacg gagatcgcga caccgatgcg gcgaaccgat
     1861 cggtcaggca cggcagccag gcggccatga agccgccata gctctgcccg gtgacgccga
     1921 tcctgccggg atcggccacg ccctcggcga ccaggtgatc gagtccggtc aggagatcct
     1981 gcgcatccat accccccatg tcgccgagca ccctctcggc gaactccgca ccccgacccg
     2041 cgctgcctcg cgggttggga cggaacacgg cgtacccgcg cgcgacgagg atcgtcgtgt
     2101 gtgcgtcccg gccgatccag ccgtcctgga acgcgcccac cggtcctccg tgcacgtgca
     2161 gaactatcgg gtacggcgca tcgccatccg gcgtcgtgag gagtccctcg atgtccagtc
     2221 cgtcggtcga ggtccatcgc agtgcgcggg tctgaccggt ggtgtgcacg acgtgctcgg
     2281 tgcccgggcc gcgagtgtcg agcagcacct cgtcgccatc ggcgccgaca cgcaccagcc
     2341 gtggcggcat gtcgtgctgc tcgagcagca ggaccacccg accgtcagcg gtaaggccgc
     2401 caaggtctgg ctggtagagg cccgtcgtcg cctgacccga ccacagctcg acacatctgg
     2461 ccgagccgac atccaccgtg cccacaaccg tgtcgagacc gcgatggccg atgaagagaa
     2521 gccggtcgtc atcgacccag cgttggtcgg tgacgtgcgt gtccgccgtc tccatcacga
     2581 ccggctcggc gccctcgcga gcgacgatga gcgtaccggc gagcaggtca cgatcgctgg
     2641 cgaagccggt gagcaccgac cagctcgatc cgtccggcga accgcacgga tgggcgagct
     2701 ggtatgtcgc ctcgtacagc ggcgagacct caccgtcgag acggatccgc acgaggcgtg
     2761 cggagtacca ggcgtcctcc cccgcccctt cgctgaccag cgcgacaacg tcgtcgccgt
     2821 accagtccgc ctcccagacg ttgagggcgg tgatcgtgca gtgcgagccg ctcgccggct
     2881 cccacacgac gagccgacga cgaccgcctt cgtgcgggaa cacctgcggg cgccacgact
     2941 cggtgacgcc accgctgacc ttgcccgacc cccagacgtc tgagatctcg gcacccgcgt
     3001 cggcgacgat gagcgcgagg cgcttccctt cccgagacca gcgggcgatc tcgaccgacc
     3061 cgcccaggtc ctcagcccgg gtcgtcttcg aggtgccctc gacgtcgatg acgacagcgg
     3121 tcgtgtgttc gccgtcgcgg ccggcgagca cgcacagccg ggtgccgtcc ggtgaccaga
     3181 ccggccacgc ggaggtctcg tccgtgtcga gcaccggtcg cgtcgagccg ggcgagtcca
     3241 cgagaacgac ctggcggcgt acgtcggcat ccggtgagtc gcggacgccc agcgtgcagg
     3301 cgacgaggtt gcggctcggg tgtggcgccg cctgcgagat gtcggcgagc ccgtccgtgg
     3361 tctcccgcga agcacgctgg tacgaagcga cccactgctc gatctgctcg aacgtctccg
     3421 tcacacgttc tcccttgcgc cggatccccc gcgaattgcg gggacttccg gcttctcggc
     3481 cggaaaaatc ccggaactgc ccgcaatccg cggttacagc cgcggtccgt aggtctcgag
     3541 cgcgttgtcg accatcaccc gctgtcggat ctgtgccggg agtgtggtga gggcgtaccg
     3601 cgggtcgtcg aagtcccagt gcgggtagtc ggagctgaac atcaaggtcc gctcggcgca
     3661 cgcgatctcg cacagcatgt gcaggtgcgc gaggcgcttc ggctccggca gcggctgggt
     3721 tgtgaaccgg acatggtcga agacgtactc gctcggtagc cggcgtaccc acggcacctc
     3781 gtcgcgcaga cccttgacgt tcttgtccag ccgccacaac acgtccggga tccagccgag
     3841 acccccttcg gtgagcacga ccttgaggtc ggggaagcgc tcgaagacgc cgtgacagac
     3901 caggctgatg aggttggcct ggaagacctg gctcaaaccc gtgtgccact cgacgtagta
     3961 cgtcggtgtg ccaccggcca acggcggcga cgtgtggaag atgccctcgc cagagttggg
     4021 atggacggtg atcggcaggc ggagctcggc cgccgcctcg tagatcgggt agtagtgccg
     4081 ttggcccatc aggatgttcg tgagcggcag gagcaccgcg acgaaccgct ggtcctcccc
     4141 tgctcgacgg atctcccggg cagccagttc cggatcctgc gcgccgacca cgatcgtgcc
     4201 gcggtaccgg tcgtcggccg ccagccaggt cgccgcgagc cagtcgttgt acgccgaggc
     4261 gatcaacgcc gccgcgtccg ggtcgggcat cgcaccgagc ccgagcacct caccaccgat
     4321 cagcaccgcc cgcgagatcc cgttggcgtc gagcagctgg tgtgctgtga acgcagggtc
     4381 cgctcccggg gccgagccgt ccggcgcacg agcgtcgtcc cggaggacac cggcctggtt
     4441 catgtacagc tggttacgcg ggatcgacac cgcctcgcgg tgacccacgg tggtgaggtt
     4501 gcgccgcccg tcgagtccga gccgccgctt cgccgcctcg gacaggaacg ggcgcaggtc
     4561 cgcgagaccc cctttcatga tcgggtggac gtcgcagtcg acgagctcgt actgctgctg
     4621 tgcgtgctcc tcggttgcag gcgcgtcgag caggtcggtc atggcgtact ccttgcgggt
     4681 tgccttgcgg gacggccccc gcaaccgtca cagtcattcc tagcatggcc atcccgccga
     4741 tccatgtgac agacaggagc gcttgatgga gcaccgggtc ggttcggtcg acgacgtccg
     4801 tcgcgccggt tgccggatcg tgcaggtcga cggacgtcgt gtcggtgtca tcagcgtcga
     4861 cgcaggcttc tacgcggtgc acgaccgctg tccgcacatg ggagcctcga tgtgcgcggg
     4921 gtcagtgggc ggcacgctcg tggccagtga gccgcacgag ctcgtctacg gcatggaaga
     4981 ccgcgtgatc cgatgcccgt ggcacggctg ggagttcgac ctcgagaccg gacggtcgct
     5041 gctcgagccc gagcggatcg gcctcaagac gtaccgcgtc acgaatgacg gccacgacgt
     5101 catcctgcac acctgaccac cgcaacgctg ggtgacccag gccggcaccc gatgtgtcag
     5161 gtgggacgct ccagtccgac gatgtgccag gtcagcgcgt tggaaaggta ccgggtctcg
     5221 agggatccgc gttcagcagc cgcggtcagg gcgacgggat cgtgcaggta ggtgcggaac
     5281 gccgtccgcc ggagtcggaa gagcaggttc tcggtcgcga acacgagccg gctgacgaga
     5341 ttgcgcggtg ggtgactgaa cacgagcagg cgggttgcat ggtccgcagc cgccgccaac
     5401 agccgctggt ggtcgcgata gcagcagaca acccggtgca ggacgacgat gtcgtgcggc
     5461 tcgaccaggt caggggtcag cgcgagatcg acctgccggc gcgtgatccg atctgccagt
     5521 cctgcctccg cagcgagcgc ccgggcgtct tcgtcgtacg agtcgacgag ctcgaggttg
     5581 gtggcgcggg cggcaccgcg acgtaacagc tcgagctgga tgtcgccgac ccctccgccg
     5641 agctccaaca cagtcgcgcc gtcgatcccc cggtcggtca gaaaggcgac gatccgtctc
     5701 gcggtcttgt ccagtccgcg cttgcggtac cgcttcgcga gatggcggga gtagcgctcg
     5761 gtgaagaggt cgccgtaccc gcgtggttcg cagcatccac ccatgtcgtg attatccgat
     5821 ggccgccgct tggtcagtgg ccgacctcga cgagcagcac gccagcggcg atgcatgcga
     5881 tcccgcagcc catgagcacc ccgagatgct cgccgaagag gaacctggac gcgaccgcag
     5941 tcagggcaac gccagcggcg gcccaggtgc cgtacgcaac gccgaggccg aggccctcag
     6001 cgagtgcgag agacaggaac gtgaaggcga ggaggtagcc agtcacgacg acgccgtacc
     6061 agatcttggc tcccgcagcg gcgagccgca gggacaacgt gccgctcacc tcggcaacaa
     6121 tggccacgct cagcaggacc caccgcatca ggttcggcgt ccgtcccggg tgggcggacc
     6181 cggacggctg gggtgggacc cgagctccac caggagcatc ccgcagacaa ccaggcccag
     6241 gccgatgacc atgacggtcg tcagcgcctc atcgaagatc agggcagaca gcaaggcggt
     6301 cgcggcgaca ccgacagcac tccagatgcc gtaggcgact ccaagtgcca tgccccgacg
     6361 caggacgaga gccagcagca cgaacgatgc gcagtagccg acagcgacca cgacgtacaa
     6421 caggtggtga ttgagtgctg ccttgagtga aagggtcgcg gtgacctcac tgaggatggc
     6481 ggcggcaagc aagagccaga tcacggtgac acctacgcac gcacgtcgat gacgtcgact
     6541 cgtcttgcgc tgacacaccc cgaccgtacg ccgacccggc acgtcggtcc gcgatcaact
     6601 cggtcccgtg ggggtttgcc tggctagcca tgcaaacgca cgggtagcca gccagatctg
     6661 acgtacgacg ggtgggcgtg catggttccc gtagcagacc gaggtcaccg cgacttgcgc
     6721 ggtgaaacga taacgttata tcgttactgt catcgttaca tcgacaggaa ggaacggtct
     6781 cttggagaac caccaccaca cgcgccggca cgacgaacat gcgggaaggc gacgccccgg
     6841 acggcatcac cggcgaggtg gccgtgcgtc ccgcggcgac gtgcgcgctg ccgtgctgga
     6901 gctgctgacc gagcagccga tgcacggcta ccagctcatg cacgccatcg ccgatcgaac
     6961 cgggggacga tggacaccga gtccaggtgc gatctatccg acgatcagcc agctcgagga
     7021 cgaggggctc gtcgtcatcg cggcggagtc cggacgaaag ctcgtcacgc tcaccgacga
     7081 gggccgagct ctcgcccagg cggatcgaga cgcaggggtc gacccgttca ctcgcttcga
     7141 gcccgccagc cccggtctcg acctgcgccg cttgagcgac gagctgcgca acgctgtacg
     7201 cgacgtggcg cgtcatggcg acagcaccat gcggtccgca actgcggcca ttctcgccga
     7261 cgcgcgcaaa cgcgtgtatc tcttgctggc agaagggtct gcggagagcg acgcgtcgta
     7321 cgtcgcccgc gcctcggtag cgacggcaaa accggaacgg tacgtcaaac agctggctgc
     7381 gcacttcggg cgccgcagcg aggtccgtgc ggagccctct gggacgcgcg tcatcttgac
     7441 cgacggcgaa tgcctgctac gtacaggtga cgactcgatc gagctcgtgg tcagcgctga
     7501 tgatccggac aggctcgaat ccgtcaagca ggtcgtcggc tcacacctcg aacgattcgg
     7561 ccagcgcgac ggcctgacgg tcagctggac ctgacgcaca cgtccgatcg ccgcctcccg
     7621 gcacaggtgg ggtcggaggt ttgcgatgtt tacagcccaa gcggcgtgtg aacatcgcaa
     7681 acctccgatg gacggagtgg gtttgccatc cgaacatcgc aaacaccagg cgggccatcc
     7741 gcagatgtcc gaggcccgcc agtcacacat caggcaccgg cgcgtcgtac gccgagccag
     7801 tcgtgactcg gtcggccggc atccttcgga gcgtggcgac gcggaaccga ccatgggctc
     7861 cctgctcaag gccttacgac cctgtcgtgg tcacctgcca ggtcgtgtac ttgtcacgac
     7921 acccggacga ggtgatggcg gtgaccgcca ggacgaatcc agctgaacac atccgagccg
     7981 gcgcggccgg tgagcggccc cgtcgacaca gacgagcatg gatcgtcgtc gcggtcgtcg
     8041 agtccacttg cgcctgcgcg gcactcctcc ttgatctgtt gatccccacc cttgtcctcc
     8101 tggcaatggc aacgctgtcg ttgctcgctc gacgcgaagg tctcgggtcg ctgggacttc
     8161 accgagtcgg aggcagcagc ctcgtcgcaa agatgttcgt gttcgcggtg gtgtggtcgg
     8221 tatttcagct cagcgtgacg atgccgatcg caaaccatct ctccgggagg caggcggacc
     8281 tcagcggctt cactgaactc caggggaacg t
//
LOCUS       DATBFZ010000087         1599 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_3005416, whole genome shotgun sequence.
ACCESSION   DATBFZ010000087 DATBFZ010000000
VERSION     DATBFZ010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3005416"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1075)
                     /locus_tag="VH419_02615"
     CDS             complement(<1..1075)
                     /locus_tag="VH419_02615"
                     /inference="COORDINATES: protein motif:HMM:NF014147.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042541.1"
                     /translation="MPTPTIETVASSASPTSSSTNPHHPPTSYAWQAWVTSPTEASVR
                     LAVPRTSSDAPLDVITVPVRATGRNWLGVGAALTDSSVELLTGRPALRALLYDPDRPD
                     GAQLNMIRLPLSATDFSTTWWTWGWNAAVGAWRPPPQAIAAADTAAAIAALRSDLFVA
                     AVPWSAPGSMKTSGSIAGGALTPGSETDFAKMLVDQVEWLRGKGLLLGALAIVNEPGY
                     SDNYPTMTMTDQQLATVGTMVKNTIGGKPELWAVDHNWSDRPRVDNVLALAPGVFDRA
                     AFHCYAGSPGEMSGLPVAAVMTECTGTSDTWTGTFRWDAANLIMGSIEAGSTGLMMWN
                     LALDPAHGPKRPGGCDNCRGLLTV"
BASE COUNT          285 a          510 c          542 g          262 t
ORIGIN      
        1 cgacggtcag taggcctcgg cagttgtcgc atcctccggg acgtttcggg ccgtgtgccg
       61 gatcgagagc gaggttccac atcatcaggc cggtcgagcc cgcctcaatg cttcccatga
      121 tcagattggc cgcgtcccac cggaaggtcc cggtccacgt gtcgctcgtg ccggtgcact
      181 cggtcatcac ggcggcaacg gggagcccag acatttcccc gggtgatccg gcgtagcagt
      241 ggaaagcggc gcggtcaaag acgcccggtg ccagggccag gacgttgtcg acgcgagggc
      301 gatcggacca gttgtggtcg acggcccaca gctcaggctt gccgccgatg gtgtttttca
      361 ccatcgtgcc gaccgtggca agttgctggt cggtcatcgt catcgtcggg tagttgtcgg
      421 aatatccggg ttcgttcacg atcgccaacg cgccgaggag aagtcccttg cctcgcagcc
      481 actcgacctg atcgacgagc atcttggcga agtcagtctc cgaaccaggg gtcaatgcgc
      541 cgccggcgat cgaaccgctc gttttcatcg acccgggcgc gctccaggga actgcagcga
      601 caaagaggtc cgatcgcagc gccgcaattg ctgctgccgt gtcggcagca gcaattgctt
      661 gcggcggagg ccgccacgcg ccgaccgcgg cgttccaccc ccaagtccac cacgtcgtcg
      721 agaagtccgt tgccgagagt ggaaggcgga tcatgttcaa ctgggcgccg tcagggcggt
      781 cggggtcgta aagaagcgct cgtagagcgg gccggccggt cagcaattcg accgaggagt
      841 cggtgagcgc cgcgccgacg cccagccagt tccgtccggt cgcgcggaca ggcaccgtaa
      901 tgacatcgag tggggcgtcc gaagaagtcc gtgggacggc gagacgaacc gaggcctccg
      961 taggtgatgt cacccacgct tgccatgcgt aagaggtcgg tgggtggtgc ggattggtgg
     1021 aactgctggt cggcgaagcg ctcgacgcaa ccgtctcgat ggtcggtgtg ggcaaggtcg
     1081 tcgtcgtggc tgccgcggcc ggttggattt gtccggtgac gtcgatgagt acgtcggtct
     1141 cggctgacga gaacacgcag acgcggcctg cgtcgcccac cctgaccagc actgaattgg
     1201 gtaccacatc gccggcgacg tagttgaggt tggaggccag agggacgggg ccgccgcagg
     1261 ggaacacggt caagtatccg gcgtcggagg gctgcactgc cgtcacgttc aacagcaccg
     1321 cggacgcatc cggggccacc ccgccacggc cgaccaggtg cacaccgatg acctgtccgg
     1381 cacctacacg accaacaccg gcgaacagtc cgtcgaccgt cgaggcgccc gagcgcgagt
     1441 ccagtaaccg caccggaccg accggcgcga ccgaaccacc cgactctggt tggatttgtc
     1501 cggtgacgtc gatgagtacg tcggtctcgg ctgacgagaa cacgcagacg cggcctgcgt
     1561 cgcccaccct gaccagcact gaattgggta ccacatcgc
//
LOCUS       DATBFZ010000088         1724 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_708981, whole genome shotgun sequence.
ACCESSION   DATBFZ010000088 DATBFZ010000000
VERSION     DATBFZ010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 1724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1724
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_708981"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..488
                     /locus_tag="VH419_02620"
     CDS             <1..488
                     /locus_tag="VH419_02620"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY7042542.1"
                     /translation="GAPAIPLEDLELEQWDSVIETSVTGAFLCTQHAFRIMKRQTPRG
                     GRIINNGSVSAGVPRPLSAPYTAAKHAITGLTRSTSLDGRPYDIACGQIDVGNASTEL
                     TEAMRTTGVLQADGTTRQEPTIDVSHIARAVVYMATLPLDANVQFITVMATKMPFIGR
                     G"
     gene            complement(1007..1273)
                     /locus_tag="VH419_02625"
     CDS             complement(1007..1273)
                     /locus_tag="VH419_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042543.1"
                     /translation="MTRFRSYWRWLGHRLRLSQPGRVFFTVALLVGVAAIAVARAHGD
                     REGGAPLVMFALLLVGVVTLDAVGRGVVAVVRRLILDGGRRRHS"
BASE COUNT          329 a          582 c          539 g          274 t
ORIGIN      
        1 tcggggcgcc ggcaataccc ctggaagatc tagagctcga gcaatgggac tcggtgatcg
       61 agaccagtgt caccggtgcc ttcctctgca cccagcacgc ctttcggatc atgaagcgcc
      121 aaacgccccg gggcggacga atcatcaaca acggctcggt ctctgcaggc gtgccccggc
      181 cgctctcggc gccgtacacg gctgccaagc acgccatcac cgggctgacg agatcaactt
      241 cgctcgacgg ccgcccctac gacattgcct gcggccagat cgacgtcggc aacgcaagca
      301 ccgagctgac cgaggcgatg cgaacgacag gcgtcctcca ggcagacgga acgacccgtc
      361 aggagccgac catcgatgtg tcccacatcg cccgagccgt cgtctacatg gcgaccctgc
      421 cgctcgacgc caacgtgcag ttcatcaccg tgatggcaac caagatgccg ttcatcggcc
      481 gtggctgagg gacgctgcac cggcttgcgc gcaaccagtt gatgcatgcg cgcgtgagcg
      541 accacggtca gtggatcgtc gtcgaacgcg cgcatggcct cggagatctc gtcgtaccgg
      601 gcctccgtca tcgcgccgtc cttgacgagg tgggctgcgg cctcgcggta cgtccagctc
      661 cagaagccct tgtgctctcc cgtgaggtag tgcgggtgat acgcgccgat gcggaggacg
      721 tcgaatcccg cctggctcgc ccaccggtgc aggcggcgac cgcccgcata atccacgccc
      781 agcgtgacat gcatcgacaa gtagtgcgcg ggttcgatcg tcatggatct cgctgatggc
      841 ccggagccgc gcatggccgg acttgccggc atcgaggacg tactcgtcgc cgtgggcggt
      901 catgcgggcg cgccttccct tgccggctgc cgaccccgtt ggcgaatcta ttcgctcgct
      961 tgccttcgga tcaacccaag gcggcatggg atggcgaacg aaatgttcag gagtgacgcc
     1021 tgcgcccgcc gtccaggatg aggcggcgaa caacagcgac gactccccgg ccgactgcat
     1081 ccagggtgac cacgccgacc agcaagagtg cgaacatcac caggggtgca ccgccctccc
     1141 ggtcgccgtg agcccgcgcg accgcaattg cggcaacgcc gactagaagc gcaacggtga
     1201 agaacactcg gccgggctgg gagagccgaa gccggtgacc gagccatctc caatagcttc
     1261 ggaatctcgt catgcgtgaa gtctaggtca gaggaacgag cgacaccttg ccccagtctt
     1321 cgggaatgcc gccggattgc ctccgtagga tcgtctcgtc tggctccttc gtagaggagg
     1381 gggagatgtg accgaccgac tccccgaacg ggcgcgtcaa cctcggcacc acatacctcg
     1441 acgccaacga actccgcatc gtcgatggga agctcacgct ccacttcagc agcgctgaac
     1501 caagagatga agatgcgaaa gcgaactggc tcccggcacc cgacgaccag ttctgccttg
     1561 ttgtccgcac gtacgttccg gaacgaagcc tcctcgacag cacctacaag cttccagacg
     1621 tagtccaatc cgagtagcgg cggcgtcagc gctgtcgtgt gggccagcgc cccggaatga
     1681 cgagtccgac gacggatcag gttgggccta gcgtctgggc gggg
//
LOCUS       DATBFZ010000089         1646 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_2023169, whole genome shotgun sequence.
ACCESSION   DATBFZ010000089 DATBFZ010000000
VERSION     DATBFZ010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2023169"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..666
                     /locus_tag="VH419_02630"
     CDS             <1..666
                     /locus_tag="VH419_02630"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY7042544.1"
                     /translation="TWGMQTAALVETHKVFLTDRWGHGRTPGTDEPFSYDDMAAETID
                     FVEQVVGGPAALVGWSDGGIVALYVSIKRPDLVTRQVLIGASFHYDGLEPGFDLGDDP
                     DADEVAVFKAMYDAVAVDPSHWPVFFAKTMKLWREQPTLTVDDVATVAVPTLVLAGDD
                     EAVKLEHTVAMYEALQNAELGIVPGTSHVLTLEKPEIVNRLILDFLAETGPPVTMAPI
                     RRA"
     gene            complement(771..1601)
                     /locus_tag="VH419_02635"
     CDS             complement(771..1601)
                     /locus_tag="VH419_02635"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY7042545.1"
                     /translation="MSDSVGQYLNEIGLVPLLTAQEERELSQIIERGREAQARLDAGE
                     KSRDLRKAVREAADAKDRFIRANLRLVVSVARRYPMPPGMELLDLIQEGNLGLEHAVD
                     KFDWRKGFKFSTYATFWIRQAIGRALDQKASLVRLPGDRSASLRAALRAVSGDSDELD
                     EEHARLHRLTTPTSLDRPVGDDDGSELIDLIADVKPGPEDMLLTREQDNLVTSLLDVL
                     DPRARHAVEQRFGLNDGRKRSYREVGDELGVTAEAARRLVKRAVNTVKEEAAARVDAA
                     "
BASE COUNT          260 a          517 c          582 g          287 t
ORIGIN      
        1 acgtggggga tgcagacagc tgccctggtc gagacccaca aggtgttcct gacggatcga
       61 tggggccatg gacggacgcc cggcaccgac gagccgttct cctacgacga catggcggcc
      121 gagacgatcg acttcgtgga gcaggtcgtc ggcggacccg ccgcactggt gggatggagc
      181 gacgggggga tcgtggcgtt gtacgtctcg atcaagcggc cagacctggt cacccgccag
      241 gtgctgatcg gggccagctt ccactacgac ggactcgagc cggggttcga cttgggcgac
      301 gatccggatg cggacgaggt cgccgtgttc aaagcgatgt acgacgcagt tgccgtcgac
      361 ccatcgcact ggccagtctt cttcgcgaag acgatgaagt tgtggcgcga gcagcccacg
      421 ttgacggtcg acgatgtcgc gaccgtggcg gtgccgacgc tcgtactcgc cggtgacgac
      481 gaggccgtca agctcgagca caccgtggcg atgtacgaag cccttcagaa cgcggagttg
      541 gggatcgtcc ctgggacgtc gcacgtgttg acgctggaga agccggagat cgtgaaccgg
      601 ctcatcctcg acttcttggc ggagaccggt ccacccgtga cgatggcccc gattagacga
      661 gcctgatctt cggcatgctg cgggcgtcgc ttcgctcctg cctccgcatg cgtgtcggcc
      721 cgacgctggc gctttccggg ccgacgtccc agcttgcgta gaccgacggg ttacgcggcg
      781 tcgaccctcg cggctgcctc ttccttgacg gtgttgaccg ctcgcttgac gagccggcgg
      841 gcggcttcgg cggtgacacc gagctcgtcg ccgacctcgc ggtagctgcg cttgcgaccg
      901 tcgttgaggc cgaaccgctg ctcgaccgcg tgacgggccc gaggatcgag cacgtcgagc
      961 aggctggtga cgaggttgtc ctgctcacgg gtgagcagca tgtcttccgg ccccggcttc
     1021 acatcggcga tgaggtcgat gagctcggag ccgtcgtcgt cacccaccgg gcggtcgaga
     1081 gaggtggggg tggtgaggcg gtggaggcgg gcgtgctcct cgtcgagctc gtcgctgtcg
     1141 ccggacaccg ctcggagcgc agcgcggagg ctggcggaac gatcgccggg gagccggacg
     1201 agactcgcct tctggtcgag cgcccggccg atggcctggc ggatccagaa cgtggcgtac
     1261 gtggagaact tgaagccctt gcgccagtcg aacttgtcga cggcgtgctc gaggccgagg
     1321 ttgccctctt ggatcagatc gagcagctcc atgccggggg gcatcggata gcggcgggcc
     1381 acgctcacga cgaggcggag gttggcgcgg atgaatcggt ccttggcatc ggctgcctcc
     1441 cgcaccgcct tgcgcaggtc acgcgacttc tcgccggcgt cgagacgcgc ttgcgcctcg
     1501 cgaccacgct cgatgatttg ggaaagctcc cgctcttcct gagcggtgag cagcggaacc
     1561 aggccgatct cgttcaggta ctgaccgaca gaatcactca tggcaaaggc cctcaacgcg
     1621 aactactggg gtgtcattcc cacctg
//
LOCUS       DATBFZ010000090         5586 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_4622954, whole genome shotgun sequence.
ACCESSION   DATBFZ010000090 DATBFZ010000000
VERSION     DATBFZ010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 5586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5586
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4622954"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..836)
                     /locus_tag="VH419_02640"
     CDS             complement(<1..836)
                     /locus_tag="VH419_02640"
                     /inference="COORDINATES: protein motif:HMM:NF022112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HEY7042546.1"
                     /translation="MSTVRRSYAARFVVALMVVGVSTACVSIPESSSVHAGRGPSAQD
                     ERPLSSNTPNGPRPGAGRKEIATGYLSAMLAFPPAPDVVREFLTPEAATAWNPEQGLV
                     VYTESSRPRLSETANFVIFSARALGSLDERGSWSTATGPRREIRAAFAMERVGGEWRL
                     RNPLPGTYVDQDYFSTYYDAFSLYFFEATNTILAADPVHMLLGDSLATSLVEDLLKGP
                     TRELQGAVGSSMPTTATVTTAVTISPSGVAEIPLSDEILQLSANDRRLFAAQLVWTLQ
                     PL"
     gene            complement(833..2551)
                     /gene="mtrB"
                     /locus_tag="VH419_02645"
     CDS             complement(833..2551)
                     /gene="mtrB"
                     /locus_tag="VH419_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020577235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MtrAB system histidine kinase MtrB"
                     /protein_id="HEY7042547.1"
                     /translation="MTSREGAAADPRTRAVASLRSARHVAATPIRQLAGLWRRSIQAR
                     VVISTVVLSTILTLLAGWILLRQVTDGLLESKEQASISQARSGAVAAQSKLDSADIAD
                     LFDIGPLVKGLVETLAPRDEARDDYTVAIIGPLAPAEGDASATGGSRSSGDIDPQKSI
                     PQDLMDSVTSQPGTWWAYSTMHFTDSSVDPVAGIVVGTQISVTGTAQRYGLFYFFPLS
                     EQQQSLLLVRSALITTGALLVVLLGIIAWLVTRQVVTPVRLARRIAERLAAGRLEERM
                     HVSGEDDIARLGTSFNQMAASLQRQIRQLEELSRVQHRFVSDVSHELRTPLTTVRMAA
                     DVLHDSRFDFDPNTARSAELLQAELDRFEGLLTDLLEISRFDAGAAVLELSDVDMRGL
                     ARRVVDANAALAESRGSRVTVSTTAAPCIAEIDVRRIERIVRNLVVNALEYGAGGDVE
                     VTVAASDDAVALTVRDHGVGLKPGEEVLVFNRFWRADPARARTRGGTGLGLSIAVEDT
                     ALHNGSLDAWGEPGVGAVFRLTLPRRAGDRPGRSPLPLVPMERKPMVVGAPYARHPHD
                     HRRNSP"
     gene            complement(2658..3389)
                     /gene="mtrA"
                     /locus_tag="VH419_02650"
     CDS             complement(2658..3389)
                     /gene="mtrA"
                     /locus_tag="VH419_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008364121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MtrAB system response regulator MtrA"
                     /protein_id="HEY7042548.1"
                     /translation="MNAGPAKTAEPVRPHRGKILVVDDDSSLAEMLTIVLRNEGFEPR
                     VCASGDRAMSVVREFKPDVVLLDLMLPGKDGVDVCKEIRAESGVPIIMLTAKSDTVDV
                     VVGLESGADDYVVKPFKPKELVARLRARLRKFEESAPEGLSIGDLVIDVAGHTVKRDG
                     QPLSLTPLEFDLLVCLARKPWQVFTREVLLEQVWGYRHAADTRLVNVHVQRLRSKVEI
                     DPENPQIVLTVRGVGYKAGEPVSTQ"
     gene            complement(3475..4935)
                     /gene="ahcY"
                     /locus_tag="VH419_02655"
     CDS             complement(3475..4935)
                     /gene="ahcY"
                     /locus_tag="VH419_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEY7042549.1"
                     /translation="MDFKVADLSLADFGRSEITLAEHEMPGLMAMRERFGESKPLSGA
                     RIAGSLHMTVQTAVLIETLVALGAEVRWASCNIFSTQDHAAAAVVVGPDGTPDDPQGV
                     PVFAWKGESLAEYWSCTERILSWPGDDGDEAAGSVSAGPNMILDDGGDATLLVHLGVE
                     YEKEGRVPSPGSTDNPELREILKLLTSSLAETSNKWTSIANGIRGVTEETTTGVHRLY
                     ERQRDGALLFPAINVNDSVTKSKFDNKYGCRHSLIDGINRATDVLIGGKVAVVCGYGD
                     VGKGCAESLRGQGARVIVTEVDPICALQAAMDGYQVATLDEVVEEADIVITATGCYKV
                     VTVDHMRRLKHQAILGNIGHFDNELDMAGLEAEPGIRRKTVKPQVDVWTFEDGKSVIV
                     LSEGRLLNLGNATGHPSFVMSNSFTNQVLAQIELFTKTDEYTTDVYVLPKHLDEEVAR
                     LHLAALGVSLTTLTEEQAKYLGIPTAGPFKSDHYRY"
     gene            complement(5067..>5586)
                     /locus_tag="VH419_02660"
     CDS             complement(5067..>5586)
                     /locus_tag="VH419_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEY7042550.1"
                     /translation="HTAAHESNRVRGNLSWWQFVRNARAPELPVVLLEDVAALLGLFF
                     ALIGVGMSLLTDNAYWDAAGTAAIGLLLVAVAVVLAIETKSLLLGEAAVAADVASIRE
                     SIRSATGVQDVIHLKTMHLGPEEILVACKISVEPTDHAMAVAHAIDEAEERIRAVVPE
                     AAYIFIEPDIQR"
BASE COUNT          883 a         1905 c         1876 g          922 t
ORIGIN      
        1 tgcaacggct gcagcgtcca gacgagctgg gccgcgaaca accggcggtc gttcgccgac
       61 agctgcagga tctcgtcgct gagtgggatc tctgcgactc cggaagggga gatcgtgacg
      121 gccgttgtga cggtcgccgt ggtcggcatc gagctgccaa ccgcaccttg cagctcgcgc
      181 gtcggaccct tcagcagatc ctcgaccaac gacgtggcga ggctgtcacc gagcaacatg
      241 tgcaccgggt cggccgccag gatggtgttg gtggcctcga agaagtacag cgagaacgcg
      301 tcgtagtagg tcgagaagta gtcctggtcg acgtacgtcc ctggcagcgg gttgcgcagt
      361 cgccactcgc cgccgactcg ctccatcgcg aaggcagccc tgatctcgcg acgggggccg
      421 gtcgcggtcg accagctgcc acgctcgtcg agcgagccca gcgcgcgagc gctgaagatc
      481 acgaagttgg cggtctcgga cagtcgtggc cggctggatt ccgtgtagac gaccagcccc
      541 tgctccgggt tccaggccgt cgccgcctcg ggtgtgagga actctcgcac gacgtcggga
      601 gcaggcggga aggcgagcat cgcgctcagg tagccggtgg cgatctcctt ccggccggcc
      661 cccggccgtg gcccgttggg tgtgttgctg ctgagcggcc gctcgtcctg cgcgctcggt
      721 ccgcgtccgg cgtgcaccga cgagctctcc gggatggaca cacaggcggt cgacacgccc
      781 acgaccatga gcgccacgac gaacctggcc gcgtacgacc gccgcacggt actcacgggc
      841 tgttcctgcg gtggtcgtgc ggatggcgcg cgtacggcgc gcccaccacc atcggcttgc
      901 gctccatcgg gacgagtggc agcggcgagc gccccggacg gtcgccggca cgccgcggca
      961 gcgtcaggcg gaagacggcg ccgacaccgg gttcgcccca ggcgtccagt gaaccgttgt
     1021 ggagagcggt gtcctcgacg gcgatcgaca ggccgagccc cgtcccgccg cgagtccgag
     1081 ctctcgccgg atcggcgcgc cagaaacggt tgaagaccag cacttcctcg cccggcttga
     1141 gcccgacacc gtggtcacgc acggtcaacg ccaccgcgtc gtccgaggcc gccacggtga
     1201 cctccacgtc acccccggcg ccgtactcga gcgcgttgac gaccaggttg cgaacgatcc
     1261 gttcgatccg ccgaacgtcg atctcggcga tgcacggcgc ggccgtggtg gacaccgtga
     1321 cccgcgaccc ccgactctcg gccagcgcag cgttggcatc gacgacgcga cgggccaggc
     1381 cacgcatgtc gacgtcggac agctccagga cagccgcccc cgcgtcgaag cggctgatct
     1441 cgaggaggtc ggtcagcagg ccctcgaagc gatccagctc ggcctgcagc agttccgccg
     1501 accgggcggt gttcgggtcg aagtcgaagc gcgagtcgtg gaggacgtct gctgccatgc
     1561 ggacggttgt cagcggcgta cgcagctcgt ggctcacgtc ggagacgaag cggtgctgga
     1621 cacgggagag ctcttcgagc tggcggatct gacgttgcag gctggccgcc atctggttga
     1681 acgacgttcc caaccgggcg atgtcgtcct caccgctgac gtgcatgcgc tcctcgagcc
     1741 gaccggcggc cagccgttca gcgatccgcc tggccagtcg caccggtgtc acgacctggc
     1801 gggtgacgag ccaggcgatg atgccgagca ggacgacgag cagcgcacct gtcgtgatca
     1861 gcgcgctccg caccagcagc agtgactgct gctgctcgct cagcgggaag aagtagaaca
     1921 ggccgtaccg ctgcgcggtg cccgtcacgc tgatctgggt gccgaccacg attcccgcca
     1981 ccggatcgac ggaactgtcg gtgaagtgca tcgttgagta cgcccaccac gtgcctggct
     2041 gcgaggtgac ggagtccatc aggtcctgcg gaatggactt ctgcgggtcg atgtcgcccg
     2101 atgaccggct cccgcccgtc gccgatgcat cgccttcggc cggtgccagc ggaccgatga
     2161 tcgcgacggt gtagtcgtcg cgtgcctcgt cgcgcggggc cagcgtctcg acgagacctt
     2221 tgacgagcgg gccgatgtcg aagaggtcag cgatgtcggc gctgtcgagc ttcgactgcg
     2281 cggccacggc gccggatcgt gcctggctga tcgacgcctg ctccttgctt tccagcagac
     2341 cgtcggtcac ctggcggagc aggatccagc ccgccagcaa ggtgaggatc gtcgagagga
     2401 ccacggtgct gatgacgaca cgggcctgga tcgaccgtcg ccacaacccg gcgagctgcc
     2461 ggataggagt cgcagcgaca tgccgcgccg agcgcagcga cgcgacggcc cgcgttcgcg
     2521 gatccgctgc tgccccctct cggctggtca cggcttgctc ctcggtcgtc gccgccgggc
     2581 tctgcctcgg ccggctcagg ttctcgcttg tggcgcttcg cgttgcgtcg tactgctcgg
     2641 gatcgatccc ttccgggtca ctgggtgctg acgggttctc ccgccttgta acccacgccg
     2701 cgaacggtca gcacgatctg tgggttctcg gggtcgatct cgaccttgga acgcaaccgc
     2761 tggacgtgga cgttgaccag ccgggtgtcg gcggcgtggc ggtaacccca cacctgctcg
     2821 agcagcacct ctcgggtgaa cacctgccac ggcttgcggg ccaggcagac gagcaggtcg
     2881 aactcgagcg gcgtgagcga caacggctgc ccatcgcgtt tgaccgtgtg accagccacg
     2941 tcgatcacca ggtcgccgat cgagaggccc tccggtgccg actcctcgaa cttgcgcagc
     3001 cgggcccgca gccgggccac cagctccttg ggcttgaacg gcttgaccac gtagtcgtcg
     3061 gcgccggact ccaggccgac caccacgtcg acggtgtcgc tcttggcggt gagcatgatg
     3121 atcggtacgc cggactcggc ccggatctcc ttgcagacgt ccacgccgtc cttgccgggc
     3181 agcatcaggt cgagcaggac gacgtccggc ttgaactcgc gcacgaccga catcgcgcgg
     3241 tcgccggacg cgcagacacg cggttcgaac ccctcgttgc gaaggacgat cgtcagcatc
     3301 tctgccaacg acgagtcgtc gtcgaccacg aggatcttgc cgcgatgcgg tctgaccggc
     3361 tcagcggtct tcgctggacc tgcgttcaca agcacacccc ttgctcgtcg cggcttggcc
     3421 accctgccga gccgtacgaa aagggtacgt cgtttcggct gctgcccggg tctgtcagta
     3481 ccggtagtgg tctgacttga aaggtccagc ggtcgggata ccgaggtact tggcctgctc
     3541 ctcggtcaac gtcgtcaacg agactccgag cgccgcaagg tggagacgag cgacctcctc
     3601 atcgagatgc ttcggcaaca cgtagacgtc tgtcgtgtac tcgtcggtct tcgtgaagag
     3661 ctcgatctgc gccagcacct ggttggtgaa cgagttcgac atcacgaacg acgggtggcc
     3721 ggtcgcgttc ccgaggttca gcagccgccc ctcgctgagc acgatcacgg acttgccgtc
     3781 ctcgaacgtc cacacgtcga cctgtggctt caccgtcttg cgcctgatgc cgggctcggc
     3841 ctcgagaccg gccatgtcga gctcgttgtc gaagtggccg atgttgccga ggatggcctg
     3901 atgcttgagt cggcgcatgt ggtcgaccgt caccaccttg tagcagccgg tggcggtgat
     3961 gacgatgtcg gcctcctcga cgacctcgtc gagcgtggcg acctggtagc cgtccatggc
     4021 cgcctgcaac gcgcagatcg ggtcgacctc ggtgacgatg acgcgcgcgc cctgcccgcg
     4081 cagcgactcc gcgcatccct tgccgacgtc gccgtacccg cacaccaccg cgaccttgcc
     4141 gccgatgagc acgtcggttg cgcggttgat gccgtcgatg agcgagtggc ggcagccgta
     4201 cttgttgtcg aacttggact tggtcacgga gtcgttgaca ttgatcgccg ggaacagcag
     4261 cgcgccgtcg cgctgccgtt cgtagagacg gtgaaccccg gtggtcgtct cctcggtcac
     4321 gcccctgatc ccgttggcga tcgaggtcca cttgttcgag gtctcggcca gggagctggt
     4381 cagcagcttg aggatctcgc gcagctcggg gttgtccgtc gagcccggcg acgggaccct
     4441 gccctccttc tcgtactcga caccgaggtg gacgagcagg gtggcgtccc caccatcgtc
     4501 gaggatcatg ttcggccctg cgctcaccga gccggccgcc tcgtcaccgt cgtcgcccgg
     4561 ccagctcagg atgcgttcag tacatgacca gtactccgcc agcgactcgc ccttccaggc
     4621 gaagaccggc acaccctgcg ggtcgtccgg cgtaccgtcc ggtcccacca cgaccgcagc
     4681 agcagcgtgg tcctgggtgg agaagatgtt gcagctggcc cagcggacct cggccccgag
     4741 cgcgaccagc gtctcgatca gtaccgcggt ctgcacggtc atgtgcagcg agccggcgat
     4801 ccgtgcaccc gacagcggtt tggactcgcc gaagcgctct cgcatcgcca tcaggccggg
     4861 catctcgtgc tcggcgagcg tgatctcgga acggccgaag tcggccaacg acaggtccgc
     4921 aaccttgaaa tccatgcggc ccaggatagg ggcagcacga acggcgacgg tcgacccggt
     4981 gcctccgagg tggcgatccc ggtccgacac gccgtcaaac tccaacgaaa cttgccacct
     5041 cggcgagagg tggcgggcgg agaaggtcag cgctggatgt cgggctcgat gaagatgtac
     5101 gccgcctccg gaacgacagc ccggatccgc tcttccgcct cgtcgatggc gtgcgcgacc
     5161 gccatcgcgt ggtcggtggg ttcgaccgag atcttgcagg ccaccaggat ctcctccgga
     5221 ccgagatgca tcgtcttgag gtggatgacg tcctgcacac ccgtggcgct gcggatgctc
     5281 tcccggatcg acgcaacgtc ggcagccacc gccgcctcgc cgagcagcag gctcttcgtc
     5341 tcgatggcga gcaccaccgc gacggcgacc agcaagagac cgatcgcggc ggtgccggcg
     5401 gcgtcccagt aggcgttgtc ggtgagcagg ctcatcccga cgccgatcaa cgcgaagaag
     5461 agacccagca gcgcggcgac gtcctccagc agcacgaccg gcagctcggg agccctggcg
     5521 ttgcgaacga actgccacca gctgaggttg ccacggactc ggttggactc gtgcgctgcc
     5581 gtgtgc
//
LOCUS       DATBFZ010000091         3133 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_3992626, whole genome shotgun sequence.
ACCESSION   DATBFZ010000091 DATBFZ010000000
VERSION     DATBFZ010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 3133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3133
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3992626"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..556)
                     /locus_tag="VH419_02665"
     CDS             complement(<1..556)
                     /locus_tag="VH419_02665"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HEY7042551.1"
                     /translation="MAPDVIVVGGGTAGCVVAAILSEDPACEVLLLEAGPDFPTVADL
                     PADVANGAEPTVNHDWGFVAERNAWGREIALPRAKLVGGCSATNAAFWFRGWPADYDG
                     WAAAGNPGWSYDDLLPTMQALGSVPLTHVSEVELAPLQSAFIEAALAAGQAAVDDHNR
                     PGPVGVGPMTRNTRDGVRMSTAQTH"
     gene            complement(660..>3133)
                     /locus_tag="VH419_02670"
     CDS             complement(660..>3133)
                     /locus_tag="VH419_02670"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042552.1"
                     /translation="LVKEVVNDNGGTAIPGDWTLNADGPTPVSGPGNSQQVTNQSVDP
                     GSYDLSETNGPPGYAASDWVCDGGMQSGASVDVALGDDVTCTITNDDQAALLTLVKVV
                     DNGTTGGTAEPADWQLTADGPTPVSGDGNSPEVTDQPVDAGTYDLSEANGPAGYAASD
                     WSCEGGTQNGASVDIAVGDDVTCTITNTAQQAHLTLVKEVVNDNGGTATPEQWTLTAD
                     GPTPVSGDGNSPEVTGQPVDPGSYDLSESGGPAGYAASDWSCDGGTQNGASVDIALGD
                     DVTCTIINDDEIAHLTLVKVVVNDNGGTAVDTDWTLSADGPTPITGTTGDPAVTDAVV
                     DAGDYALTEDGPSGYAASDWVCDGGDLTGGTVTVPNGGDVTCTITNDDQQAQLTLVKV
                     VDNGDTGGTAVPSDWTLSADGPTPITGAGNSAEVTDQPVDAGSYDLSEADGPDGYTAS
                     AWECVGGSQSGASVDIAVGDDVTCTITNTAQPSRWTLEKTSDPQSPSTVNPGDEITYT
                     ITASKLGGVDPTGVVVTDDLSSVLDNATLVAGSIDPSTGTATQNGNQIVWDIGTLSGD
                     ETLSYRVRVNDGAYGVQLRNVASGTAVTPPEECPAPAELRPVARAFIAARRAATWSAI
                     ANPKAAAEDPCSTVHRTPKWTLSKASDPTSGSTIDPGSVVTYTLTATNVSDAEVNHAV
                     VHDDLSNVLNHATLIAVPAGATLNGTTLTWNVPPMPANGDQETLTYSVRIDADAADVT
                     IGNVATPGAGGSCVTACETTHKTPATLPTQTENPPPPGQPPSGPLVNTGGPSEALVWA
                     GLLLLLVGGGGIYFATRRRRSDHAG"
BASE COUNT          457 a         1115 c         1009 g          552 t
ORIGIN      
        1 ggtgcgtctg tgccgtgctc atgcgtacgc cgtcgcgggt gtttcgtgtc atcggcccga
       61 ccccgacggg tcctggcctg ttgtggtcgt cgactgctgc ctgacctgcg gcgagggcgg
      121 cctcgatgaa cgccgactgg agtggcgcca actccacctc cgagacgtgg gtcaggggaa
      181 cggacccaag ggcttgcatg gtcggaagca ggtcgtcgta cgaccacccc gggttgccgg
      241 cggcggccca gccgtcgtag tcggccggcc acccgcggaa ccagaaggcg gcgttcgtcg
      301 cagaacaccc tccgacgagc tttgcccgcg gcagggcgat ctcacgcccc caggcattcc
      361 tttcagcgac gaagccccag tcgtggttca cggtcggctc ggcgccattc gcgacgtcgg
      421 ccggaaggtc cgccacggtc gggaagtcag gccctgcctc cagcagcaac acctcgcatg
      481 ccgggtcttc gctcaagatc gcagcgacca cgcaccccgc ggttccgcca ccgaccacga
      541 tcacgtccgg cgccataccc gccccctcgg ctcgccatga cggtacacct cgatccagcg
      601 gggatgtcga gccatgcgtg agtcgacgca gccgggctgc gtcgactcac gcgtcgggac
      661 taccccgcgt ggtctgacct acggcgcctg gtggcgaagt agatgccacc accaccgacc
      721 aggagcagga ggagcccggc ccacaccagg gcctcggacg ggccaccggt gttgaccagc
      781 gggccgctcg gcggctgacc cggcggaggc gggttctcgg tctgggtcgg cagcgtcgcc
      841 ggcgtcttgt gcgtcgtctc gcaggcggtg acgcacgagc cgcccgcacc aggcgtggcc
      901 acgttgccga tcgtgacgtc ggccgcgtca gcgtcgatcc gcacgctgta ggtcagcgtc
      961 tcctgatcgc cgttggcagg catcggcgga acgttccacg tcagtgtcgt cccattcagc
     1021 gttgcacccg ccggtacggc gatgagcgtc gcgtggttca gcacattgga caggtcgtcg
     1081 tgcacaacgg cgtggttcac ctcggcatcc gacacgttcg tcgccgtcaa cgtgtaggtc
     1141 acgacactgc caggatcgat cgtcgagccc gatgtgggat cgctcgcctt gctcaacgtc
     1201 cacttcgggg tgcggtgcac ggtggagcac gggtcctctg cggccgcctt cgggtttgcg
     1261 atcgccgacc aggtagcagc acgccgcgca gcgatgaagg cccgagccac cggccggagc
     1321 tccgcaggag ccgggcactc ctctggcggt gtcacagccg ttccggaggc gacgttccga
     1381 agctgtacgc cgtacgcgcc gtcgttgacc cgcacccggt agctcaacgt ctcgtcacca
     1441 ctgagcgtgc cgatgtccca gacaatctgg ttgccgttct gagtcgccgt gccggtgctc
     1501 ggatcgatgc tgcccgctac cagggtggcg ttgtcgagca ccgaggacag gtcgtcggtg
     1561 acgaccacac ccgtcgggtc gacaccgccc agcttgctgg cggtgatcgt gtacgtgatc
     1621 tcgtcacccg gattcaccgt cgatggcgac tgcgggtcgc tggtcttctc gagcgtccac
     1681 cgggaaggtt gggcggtgtt ggtgatcgtg caggtcacgt cgtcgccgac ggcgatgtcg
     1741 accgacgcgc cactctggct tccaccgacg cactcccacg ctgacgccgt gtacccgtcc
     1801 ggaccgtccg cctcggacag gtcatagctg ccggcatcca cgggttgatc ggtgacctcg
     1861 gcactgttgc cggcaccggt gatcggcgtc ggaccatctg ctgacaacgt ccagtccgaa
     1921 ggcaccgccg taccgcccgt gtcgccgttg tcgacaacct tgaccaaggt caactgtgcc
     1981 tgctggtcgt cattggtgat cgtgcacgtc acgtccccgc cgttgggcac cgtgaccgtg
     2041 cccccggtca ggtcgccgcc gtcacagacc cagtccgaag cggcgtaccc cgacggcccg
     2101 tcctcggtca gggcatagtc accggcatcc acgaccgcat ccgtcaccgc cgggtcgccg
     2161 gtcgtgccgg tgatcggcgt cggaccatcc gccgacaacg tccagtcggt gtcaaccgcg
     2221 gtcccgccgt tgtcgttgac gaccaccttc accaacgtca ggtgggcaat ttcgtcgtcg
     2281 ttgatgatcg tgcacgtgac gtcatcgccc aaggcgatgt cgaccgacgc gccgttctgc
     2341 gtgccgccgt cacagctcca atccgatgcg gcatacccgg ccgggccacc cgactccgac
     2401 aggtcgtagc tgcccggatc gacgggctgg cccgtcacct ccggactgtt gccgtcaccc
     2461 gagaccggcg tcgggccgtc ggcggtcaac gtccactgct cgggggtcgc ggtaccaccg
     2521 ttgtcgttga ccacttcctt caccagcgtc aggtgcgcct gctgcgccgt gttcgtgatc
     2581 gtgcaggtca cgtcgtcgcc gacggcgatg tcgaccgacg cgccgttctg cgtgccaccc
     2641 tcacagctcc agtccgaagc ggcgtagccg gctggaccgt tcgcctcaga gaggtcgtag
     2701 gtaccggcgt cgaccggctg gtcggtgacc tccgggctgt tcccatcgcc ggacaccgga
     2761 gtggggccgt cggcggtcag ctgccagtcg gcgggctcgg cagtaccacc cgtcgtgccg
     2821 ttgtcgacga ccttcaccaa cgtcagaagc gccgcctggt cgtcgttcgt gatcgtgcac
     2881 gtgacgtcat cacccaacgc gacgtcgacc gacgcaccac tctgcatccc gccatcacac
     2941 acccagtccg acgcggcata acccggcgga ccgttggtct ctgacaggtc atagctccct
     3001 ggatccaccg actggttcgt gacctgctgg ctgtttccgg ggccagagac cggggtcgga
     3061 ccgtccgcgt tcaacgtcca gtcgccaggt atggccgtcc caccgttgtc gttgaccact
     3121 tccttcacca gcg
//
LOCUS       DATBFZ010000092        18740 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_1693037, whole genome shotgun sequence.
ACCESSION   DATBFZ010000092 DATBFZ010000000
VERSION     DATBFZ010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 18740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18740
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1693037"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1267)
                     /locus_tag="VH419_02675"
     CDS             complement(<1..1267)
                     /locus_tag="VH419_02675"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY7042553.1"
                     /translation="MLSSEPIAAQGTLDPTNVYTPIPIAILTDLVTRSLTQYAYDPAS
                     GGMTLIPDLATNLGTPNDDFTEWTFTIRDGVKYEDGTPVTAADVALGIERSFDRKAFP
                     DGPTYSNDYFLDGDRYQGPYRSGTTYDGITVEGNTLTLKMAHPFPDLPYYASFPGTGP
                     IPEQGRDLARYAVHPVATGPYKFAGDLSDTSLTLVRNPYWDARTDPGRHQYVDGFRFA
                     FDTLSVQQVDATILGDTSAGATTISVDDPLPADYRRALQQDQLAAASRPCTHAWRPDF
                     RDPVTSDIRVRKALGYAFPYDAYIAAKGLAFGITALRANTLLPPLTPGRLEYNVLGTD
                     PGETDPRRARQLLAQAGYAPGEAEIKFAYETDDPYSVAAKGVLVHALERAGFKVSPRP
                     TGSFEEFLAVEADPHAPINMRIGFGGWCAD"
     gene            1654..2838
                     /gene="alr"
                     /locus_tag="VH419_02680"
     CDS             1654..2838
                     /gene="alr"
                     /locus_tag="VH419_02680"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008362439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEY7042554.1"
                     /translation="MSASPAESPASQPDRAVRAEAVVDLDAISDNVAALKDHVDRREF
                     IAVVKADGYGHGIVPAALAARAGGAEWLGVAFLDEALLLRKAGDKGSILCWLAVPGER
                     YVEAVRAGVEVSAYSVDQLEAIADAGRMAGSPAAVQLKLDSGLGRGGADPDHWEELVS
                     TAVRLRDANVLDVTGVWSHLACSDEPGHPSIQAQTTTFEAGLAHALDQGLEPAHIHLA
                     NSGGALATPSTWFTTVRVGIAIYGVSPFGDGSSPVPLQQAMTLRGRVALVKEVGPGHG
                     VSYGHTYVTSGPTRLALVPLGYGDGVPRHASSRAQVWLNDRRHRIAGRVCMDQFVLDV
                     GDAPAAAGDEVVLFGDGRQGEPTAHDWAVAAETIGYEIVTRIGSRVPRTYVGDVVSGL
                     PR"
     gene            2835..3905
                     /locus_tag="VH419_02685"
     CDS             2835..3905
                     /locus_tag="VH419_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020578634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY7042555.1"
                     /translation="MSEPDGTSPGTLKRVVGIAGASVGVLAAGAAAGIFAERKLVARR
                     RAAAAQPFGSVRGERRTVVADDMLELYAEVDPVDSGRDDLTVVFVHGYALNLDCWHFQ
                     RLATRGEHRMVFYDQRSHGRSARSRPEHCTFEQLGRDLVCVVEQLVPDGPVILVGHSM
                     GGMTIMALAEEHPEWFGDKVAGVGLISTSAGGLRALSLGLPGLPGRLLQQVSPALMAT
                     MARAPQLVERGRRVGSDIGFVVTRRLAFGTPVPQEYVDFTDEMLAATPVDVVADFFPG
                     FDTHDRYAALDIIGGVPTVVIAGGRDHITSIEHTRRMVEHMPAAELVELHQAGHMVLL
                     ECHDEVTKALRRLVERAVASRA"
     gene            3898..4896
                     /gene="tsaE"
                     /locus_tag="VH419_02690"
     CDS             3898..4896
                     /gene="tsaE"
                     /locus_tag="VH419_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007078585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEY7042556.1"
                     /translation="MRDRDGPNVGGPTEHSLDVVEVGPDAATVIRDVIHAAFAARPHL
                     DPPSTALAETEESVAAVLASYGGLLCHVDGVPAGAVLFEADASALALRRVSVVPHFQG
                     RGVATAIVGVAEEVAADRGFDDVTLHARTELPATLVFWSRRGYRELLRSGSHVVFGKA
                     LPVELPVASAADMRAVGEALAGLCRAGDVLLLTGELGAGKTTLTQGLGAGLGIRGDIT
                     SPTFVISRLHPSLVDGPALLHVDAYRLGDEAELDDLDLDAFVDGSITVVEWGEGIAEA
                     LSEDRLRVDITRTRGTDATIGLLTALDDARTVSVTPVGARWVGAAVRSHLSAFVEP"
     gene            4950..5588
                     /gene="tsaB"
                     /locus_tag="VH419_02695"
     CDS             4950..5588
                     /gene="tsaB"
                     /locus_tag="VH419_02695"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020578637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEY7042557.1"
                     /translation="MLLAFDTATHAVTVALHTGTPDGEVLAESTVVDALRHGELLAPG
                     IRQVLADAGCTPRDLTGIAVGVGPGPFTGLRVGIVTARTMGAALGIPVHGVCTLDVIA
                     AAVRDRDDGFRVATDARRKEVYWAAYDATGKRSTVPSVDRPEDVPYDGPVAGRGARLY
                     SESFPQPIEPENPDAADLATLIASDAVEALAPEPLYLRRPDAAAPGQRKRVL"
     gene            5585..6046
                     /locus_tag="VH419_02700"
     CDS             5585..6046
                     /locus_tag="VH419_02700"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY7042558.1"
                     /translation="MKIRPATEHDLDAVVVLELEVFGSAAWSPRQVEEEFSGLGDTRA
                     IWLAETAGEHGGLTPVGYAAGRFVADVADVQRVAVLRSRRRHGVGQQLLDEVVAEAQR
                     RGCDRLMLEVADDNAAALALYGANGFTEMDRRPQYYANGADALILGRPVDG"
     gene            6039..7082
                     /gene="tsaD"
                     /locus_tag="VH419_02705"
     CDS             6039..7082
                     /gene="tsaD"
                     /locus_tag="VH419_02705"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016456573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEY7042559.1"
                     /translation="MADLPLVLGIETSCDETGVGLVRGNDLLADAVASSVDEHARFGG
                     VVPEVASRAHLEAMVPTIERSCRDARVSLSDVDAIAVTSGPGLAGALLVGVAAAKALA
                     LTLDVPLYGVNHLSAHVAVDQLEHGPLPEPCVALLVSGGHSSLLLVDDVTAGVEPLGA
                     TVDDAAGEAFDKVARLLGLPFPGGPHIDRAARDGEVVIDFPRGLSSGRDLERHRFDFS
                     FSGLKTAVARWVEARELSGEPVPVADVAASFQEAVCDVLTRKAVDACVRSGAADLVIG
                     GGVAANSRIRVLAEERAGRHGIRVRVPRPGLCTDNGAMVAALGSELVRRGRTPSAYDI
                     PADSSMPLTTVVA"
     gene            complement(7094..8368)
                     /locus_tag="VH419_02710"
     CDS             complement(7094..8368)
                     /locus_tag="VH419_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042560.1"
                     /translation="MTDMQPPNNGSGADLEADVRQTLQRHAGDAPHLDLTESSLRRAR
                     RFRLRRRLAVTAAAAAVVAVCVPVAATIVDDTRSADVADGPAPSRAATSPVAPVTDIA
                     LAGLALGPAPAVPYIVDAQFVHAGRHSDLSAIATTGSGSKHSVVDAATFSDGVGGFVA
                     DSDQRLEFRATRATPLPSARAATSPAIDHNGAVAFAVNGVDVDGRPAQTGTVLYADAL
                     SAQPSYAYTHQLDVKQVMDVQDRVVLFNAATPDGTQVVGSADLASSGPGSVQQPWPNV
                     VSLSAADQAVGLMVGRTTDMGEGEHQCSAMLSTDGATELWRSCQWRPTEFSPDGSRVF
                     AVSASAAGDDVRSGAILDAKDGTVIRAFTTAGTFGRATFEGDNALDIITIQDGESAIV
                     RCFEDGNCQLAAAARPAETGSLVVPYQLTANP"
     gene            complement(8365..8934)
                     /locus_tag="VH419_02715"
     CDS             complement(8365..8934)
                     /locus_tag="VH419_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020384898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigE family RNA polymerase sigma factor"
                     /protein_id="HEY7042561.1"
                     /translation="MSALTTAPTGELVWRAVSRGGVDQSQADFVEFVRARQHALLRFA
                     YLLTSDHHTAEDLVQTALAKTYLSWNKLRDRGALDAYVRRIILNENASLWRRAWRRQE
                     RSAGELPESAVHDLAAEDRDALWHVVQQLPPKQRAAVVLRYYEDLSEADTAAILGCSV
                     GNVKSQTSRAISTMRAAVTADPGLLGHEP"
     gene            complement(9019..9570)
                     /locus_tag="VH419_02720"
     CDS             complement(9019..9570)
                     /locus_tag="VH419_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042562.1"
                     /translation="MRRILTTTALGIGFALLATSSVLSVSSASSIAIGADAALRSEQT
                     SSSSSHRVRCGADMHGEVATRVPRAWTRTVHRVDRCVWVSPSADQRLVLLTDAAPMRA
                     ERRRRVSGSVGDYVEHSWHRARVSGNPGHLWDFGCTPDSRRFRVREVGTHSFRVIYRA
                     KHGTFGAHRHIFVLARHHLALSG"
     gene            complement(9667..10860)
                     /locus_tag="VH419_02725"
     CDS             complement(9667..10860)
                     /locus_tag="VH419_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008362429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEY7042563.1"
                     /translation="MDPVAFGFLLTPAGQALLRRTEAAYDEGAEGHNELALARALRRE
                     YDAGHVSAALTQVELRRRASAKFGDQAARMYFTPDGLEQATHPAVADYRAVRLAALVD
                     APDAALVDLGCGIGSDLVSFARHGFRVVGFERDRATAAVAAANLSSFGLPGHVEVGDV
                     TAVDHLLFDVAFADPSRRSASGRTFDPHSYAPPWSFVEDLLSRRAVVKVAPGIPHAIV
                     PDDVETEWVSLDGQLKEATLWSGFAPHGRARATLLRSGVPPATLTDRDTAADPVVRSV
                     GRYVYEPDDAVIRAHLVTTVCALVDGWLLDEHVAYVSADRLVQSPFAAAYEVLDVLPF
                     KERQLRAALRTRDVGPLTIKKRGVRVTPEALRTRLALSGTRPATLVLTRTPHSAVALL
                     VDRRR"
     gene            11053..11349
                     /gene="groES"
                     /locus_tag="VH419_02730"
     CDS             11053..11349
                     /gene="groES"
                     /locus_tag="VH419_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009647648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEY7042564.1"
                     /translation="MSVSIKPLEDRILVRTLEAEETTASGLVIPDTAKEKPQEGEVLA
                     VGPGRIDDSGNRVPLDISVGDKVIYSKYGGTEVKYSGEDYLILSARDVLAVIEK"
     gene            11502..13127
                     /gene="groL"
                     /locus_tag="VH419_02735"
     CDS             11502..13127
                     /gene="groL"
                     /locus_tag="VH419_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757077.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEY7042565.1"
                     /translation="MPKILEFDENARRALERGVDSLANTVKVTLGPRGRYVVLDKKWG
                     APTITNDGVTVAREVELDDPFENLGAQLAKEVATKTNDVAGDGTTTATVIAQAMVHEG
                     LRAVAAGSNPMSLKRGIDKAVEAINAELGNVSREVDSTEDMAHVATISARDSRIGELI
                     AQAFDKVGKDGVITVEESNTLGTELEFTEGMQFDKGYISPYFVTDAERMEAVIEDPYI
                     LVNQGKISAINDLLPLLEKVIQAGKPLFIIAEDVDGEALSTLVVNKIRGTFHAAAVKA
                     PAFGDRRKAMLQDIATLTGAQVVSPDVGLKLDQVGLDVLGTARRIVISKDDTTIVDGS
                     GDAAEVDARVAQIKNEIENTDSDWDREKLQERLAKLAGGVCVVKVGAATEVELKEKKH
                     RIEDAVSATRAAIEEGIVAGGGSALVHSAAILGDNLGLTGDDAVGVRIVRRALDEPLR
                     WIAENAGDQGYVVVSKVREIGAGNGFNAATFEYGDLVSQGVLDPVKVTRSALSNAASI
                     AGMLLTTETLVVEKPEEAEPEAGGHGHGHGHGH"
     gene            13702..14340
                     /gene="shbA"
                     /locus_tag="VH419_02740"
     CDS             13702..14340
                     /gene="shbA"
                     /locus_tag="VH419_02740"
                     /inference="COORDINATES: protein motif:HMM:NF007230.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor ShbA"
                     /protein_id="HEY7042566.1"
                     /translation="MTPRAGSSPIASIAPPTDLAGLACGAQCGDKVLVEALLTRVRLI
                     AHRYSRSRLLHFPGGQQLADDVAQDICLAVLRALPRYRDQGRPFEAFVYGVASRKVAD
                     AVRALSRHPQPIDDLPDEVDSAPTPEDSAVHRSEVESAARLLAALPKTLREVMLLRVA
                     TGLTAEETGAALGMTAGAVRVAQHRALAKMREHAKRLDRIPANCGSSQAEAP"
     gene            14395..15912
                     /gene="guaB"
                     /locus_tag="VH419_02745"
     CDS             14395..15912
                     /gene="guaB"
                     /locus_tag="VH419_02745"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012923583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEY7042567.1"
                     /translation="MDLTSAGVPDKFATLGLTFDDVLLLPGETDVVPSEIDTTTRLTR
                     DISVKIPLVSSAMDTVTESRMAVAMARQGGLGVLHRNLSVDEQAGQVDLVKRSESGMI
                     TNPVTVGPDVTLDDLDELCARYRVSGLPVVDDEGVLLGIITNRDLRFVPMSEWSTRRV
                     REVMTPMPLVTAPAGISSEDAAALLAKHKVEKLPLVDENDRLSGLITVKDFVKSEQYP
                     NATKDQDGRLMVGAAIGYFGDAWDRAAALVEAGVDVLVADTAHGHVRVMLDMVRRLKT
                     DAATRHVQVIGGNVATRAGAQAFVDAGADGVKVGVGPGSICTTRIVAGVGAPQVTAIH
                     EASLACRPAGVPVIGDGGLQYSGDIAKALVAGADTVMLGSLLAGCEESPGDLVFVNGK
                     QFKSYRGMGSLGAMSSRGRKSFSKDRYFQAEVTSDDKLVPEGVEGQVPYRGPLAAVAH
                     QLVGGLHQSMFYVGARTIGELQERGRFTRITAAGLSESHPHDIEMTVEAPNYSGR"
     gene            15915..17018
                     /locus_tag="VH419_02750"
     CDS             15915..17018
                     /locus_tag="VH419_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GuaB3 family IMP dehydrogenase-related protein"
                     /protein_id="HEY7042568.1"
                     /translation="MDIEIGRAKRGRRAYAFDDIAIVPSRRTRDPEEVSVAWQIDAYR
                     FEIPILAAPMDSVMSPETAIAFGKAGGLGVLDLEGLWTRYDDPTPLLADVASLRGHEA
                     TSRLQQIYAEPIKPELMVERVKQMRDSGITVAGALSPQRTKEHAQSVIDAGLDLFVIR
                     GTTVSAEHVSGQAEPLNLKQFIYELDVPVIVGGCATYQAALHLMRTGAAGVLVGFGGG
                     AAHTTRTVLGLAVPMATAVADVAAARRDYLDESGGRYVHVIADGSIGKSGDIAKAIAC
                     GADAVMIGSPLARAEEAPGGGYHWGAEARHSTLPRGERVEIGTIGTLDQILHGPSLVS
                     DGTMNLVGALKRSMATTGYTELKEFQRVEVTVE"
     gene            17015..17830
                     /locus_tag="VH419_02755"
     CDS             17015..17830
                     /locus_tag="VH419_02755"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEY7042569.1"
                     /translation="MNLSVLRVAAVQAESRAGDVRHNVAIAARWVAKAAAEDAPLVVL
                     PELFLPGYDPKTLREDPDGCDVTVDDPRLGPLSEAAASAGVTLVVGASVREGERRLLS
                     ALVFSTTGDIAATYAKQHLWDAERGIFTPGTHGTSLVFRGWDLGLGICYDGCFPEHAR
                     AATDAGALVYVCPSAYVVGSEHRRDVYYAARAIDNGIYVVFAGLVGPCGELEFSGGTA
                     VYDPQGRTLSRVESGEGMVVVDLDPEAIAEARRINPYERDRVSDTEPRHILPL"
     gene            17983..>18740
                     /locus_tag="VH419_02760"
     CDS             17983..>18740
                     /locus_tag="VH419_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012921751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEY7042570.1"
                     /translation="MPRDPDVGVDGPASEALLKLGRFFTRWDESEDGRAVFREGGRAG
                     DVFYRDRWSHDKVVRSTHGVNCTGSCSWKVYVKDGIITWETQQTDYPSVGPDRPEYEP
                     RGCPRGAAFSWYTYSPTRVRFPYVRGVLLDMYREARNRLGDPVVAWAEITGDSERRRR
                     YQQARGKGGLVRASWREAVEIAAAAHVHTIKTYGPDRCAGFSPIPAMSMVSHCVGTRF
                     IQLIGGVMTSFYDWYADLPVASPQVFGDQTDVPES"
BASE COUNT         2882 a         6374 c         6550 g         2934 t
ORIGIN      
        1 agtccgcgca ccaaccgccg aagccgatgc gcatgttgat cggcgcatgg ggatcggcct
       61 ccaccgcgag gaactcctcg aacgagccgg tggggcgggg cgagaccttg aacccggcac
      121 gctccaacgc gtgcaccagc acgcccttcg cggcgaccga gtaggggtcg tcggtctcgt
      181 aggcgaactt gatctcggcc tcgccgggcg catacccggc ttgggccagc agttgcctgg
      241 ccctccgtgg gtcggtttcc ccggggtcgg tccccagcac gttgtactca agtcggccag
      301 gagtcagcgg aggcaacaat gtgttggccc gaagcgcagt gatgccgaac gcgagcccct
      361 tggcagcgat gtaggcgtca taggggaagg cgtacccgag ggctttccgt acgcggatgt
      421 cggacgtgac ggggtcgcgg aagtccggtc tccacgcgtg cgtgcacggt cgggaggccg
      481 cggccagctg atcttgttgc aaggccctgc gatagtcggc aggcaaggga tcgtcgacgg
      541 aaattgtcgt cgcaccagca ctggtgtcgc ccaggatggt cgcgtcgacc tgttggaccg
      601 acaacgtgtc gaacgcgaag cggaagccgt cgacgtactg atggcgtcct gggtctgtgc
      661 gtgcgtccca gtacgggttt ctgacgaggg tcagggaggt gtccgagagg tcgccggcga
      721 acttgtacgg gcccgtcgcg accggatgga cggcatatct cgccagatcg cggccttgct
      781 ccgggatagg gccggtgccc ggaaacgatg cgtaataggg gagatccggg aaggggtgcg
      841 ccatcttcaa cgtcagcgtg ttgccctcca ccgtgatccc gtcgtacgtg gtgcctgacc
      901 ggtacggtcc ctggtaacgg tcgccgtcga ggaagtagtc gttgctgtag gtgggaccgt
      961 cagggaaggc cttccggtcg aacgagcgct cgatccccaa cgcgacgtcc gctgccgtga
     1021 ccggcgtacc gtcctcgtac ttgacgccgt cccggatcgt gaacgtccac tcggtgaagt
     1081 cgtcgttcgg tgtgccgagg ttcgtcgcga ggtcagggat cagcgtcatg ccaccgctgg
     1141 ccgggtcgta cgcgtactgc gtgagcgagc gggtgaccag atcagtaagg atggcgatcg
     1201 gtatcggcgt gtagacgttg gtcggatcca gcgtcccctg ggccgcgatg ggttcgctgc
     1261 tgagcaccgt gacgaccccg ccacgtcggg caccaggaat cgccgcagcc ggcggctgac
     1321 ggtcagggtc gatcgccgac gcgggagaac tgggatttcc agctccggat gtcgggttct
     1381 gagcaggtcg gtcggcgttg tcgtcggtac aggctgctcc cagcagcaag agcagtaccg
     1441 acgacgcaat cagacacgat cgggtcgacg tacgtgatcc tccttgccgc ttcacccgat
     1501 gcgttgatac tccgtcgagc ccgatctgtc gagccgcctc tcagcggctt ggtcaacggc
     1561 gtaccgaggt ggcaatcgac gtccgacacg ccgtcgaaca gcaacaaaac ctgccacctc
     1621 ggcgaagccg agggggctga cagaatgagg gtgatgtctg cttcccccgc ggagagtccc
     1681 gcctcgcagc cggatcgggc ggtccgtgca gaggcggtcg tcgacctcga cgcgatcagc
     1741 gacaacgtcg ctgcgctgaa ggaccacgtc gacaggcggg agttcatcgc cgtcgtcaag
     1801 gccgacgggt acggccacgg catcgtgcct gcggctctgg cggcgcgggc cggtggtgcc
     1861 gagtggctcg gtgtggcgtt cctggacgaa gccctgctgc tgcgcaaggc cggcgacaag
     1921 ggcagcatcc tctgctggct cgcggtgccc ggtgagcggt acgtcgaggc cgtccgagcc
     1981 ggcgtcgagg tgtcggcgta ctcggtggac cagctcgagg cgatcgcgga cgccggtcgg
     2041 atggccgggt cgccggccgc cgtgcagctg aagctcgact cgggactcgg gcgtggtggt
     2101 gcagaccccg accactggga ggagctcgtg tcgaccgccg tccgactgcg cgacgccaac
     2161 gtgctggacg tgaccggcgt ctggtcccac ctcgcgtgtt ccgacgagcc gggtcacccc
     2221 tccatccagg cacagacgac gaccttcgag gcggggctgg cacatgcgct cgaccagggc
     2281 ctcgagcctg cgcacatcca tctcgccaac tcaggcggtg cgcttgcgac gccgtcgacg
     2341 tggttcacga cggtgcgtgt cggcatcgcg atctacgggg tgtcgccgtt cggcgacggc
     2401 tcctcgccgg tgccactcca gcaagccatg acattgcgcg gccgcgtcgc gctggtgaag
     2461 gaggtgggtc ccgggcacgg cgtctcgtac ggccacacgt acgtcacctc ggggccgacg
     2521 aggctcgcgc tcgtcccgct cgggtacggc gacggtgtcc cacgccatgc atccagccga
     2581 gcccaggtct ggctgaacga tcgccgtcac aggatcgccg gccgcgtgtg catggaccag
     2641 ttcgtgctcg acgtgggcga tgcaccggct gcagccggtg acgaggtggt gctcttcgga
     2701 gacggtcgcc agggggagcc caccgcgcac gactgggcgg ttgcggcgga gacgatcggc
     2761 tacgagatcg tcacccgcat cgggtcccgc gtgcctcgca cgtatgtcgg tgacgtcgtg
     2821 agcgggctgc ctcgatgagc gagcccgacg gcacgtcgcc cggaacgctc aagcgagtgg
     2881 tgggcatcgc aggggcctcc gtcggcgtac tggcggccgg agccgcggcc ggtatcttcg
     2941 ccgagcgcaa gctggttgcc cgccgccgtg ccgccgccgc ccagccgttc ggctcggtgc
     3001 gcggtgagcg tcgtaccgtc gtggccgacg acatgctcga gctgtacgcc gaggtcgacc
     3061 cggtcgattc ggggcgcgac gacctcaccg tcgtcttcgt gcacggctac gcgctcaacc
     3121 tcgactgctg gcacttccag cggctcgcga cgcgaggcga gcaccggatg gtgttctacg
     3181 accagcgttc gcacggtcgg tcagcccgct cgcggccgga gcactgcacg ttcgagcagc
     3241 tcggccgcga tctggtctgc gtcgtcgaac agctcgtccc cgatggtccg gtcatcctgg
     3301 tcggccactc catgggcggc atgacgatca tggcgctcgc cgaggagcac cccgagtggt
     3361 tcggtgacaa ggtcgccggc gtcggcctca tctcgacgag cgccggcggg ctgcgggcgt
     3421 tgtcgttggg tctgcccggt ctccccggca ggctgctgca gcaggtctcc ccggccctga
     3481 tggcgacgat ggcccgcgcg ccgcagctcg tcgaacgcgg ccgtcgggtc ggttccgaca
     3541 tcgggttcgt ggtcacgcgg cggctggcgt tcgggacgcc ggtgccgcag gagtacgtcg
     3601 acttcaccga cgagatgttg gccgcgactc ctgtcgacgt ggttgcggac ttcttcccgg
     3661 gcttcgacac ccacgacagg tacgccgcac tggacatcat cggtggcgtg ccgacggtgg
     3721 tcatcgccgg cgggagagac cacatcacct cgatcgagca cacgcgccgg atggtcgagc
     3781 acatgcccgc ggcggagctg gtcgagctcc accaggcggg ccacatggtg ctgctcgagt
     3841 gccacgacga ggtcacgaag gcactccggc ggctcgtcga gcgtgcggtc gcgtcccgtg
     3901 cgtgaccgcg atggcccgaa cgtcggcggc ccgaccgagc acagcctgga cgtcgtcgaa
     3961 gtcggcccgg acgccgccac cgtcatccgc gacgtcatcc atgcggcgtt cgcggcccgg
     4021 cctcacctcg acccgccgag caccgcgctc gcggagaccg aggagtccgt ggcggctgtg
     4081 ttggcgtcgt acgggggtct gctgtgtcac gtggacggcg taccggctgg tgcagtcctg
     4141 ttcgaggccg acgcgtctgc cttggcgctg cgccgggtca gtgtcgttcc gcacttccag
     4201 ggccgaggcg tcgccaccgc gatcgttggt gtggctgagg aggtcgcggc agatcgcggg
     4261 ttcgacgacg tgacgttgca tgcccgcacc gagctgcctg ccacattggt gttctggtcg
     4321 cgtcgcgggt accgcgagct gcttcgctcg ggctcccatg tggtgttcgg caaggcgctg
     4381 cccgtcgagc tccccgttgc ctccgcggcc gacatgcggg cggtcggcga agcactggca
     4441 ggactgtgcc gcgcggggga cgtgctgctg ttgacgggtg agctgggggc aggcaagacg
     4501 acactgaccc agggtctggg agcgggactg gggatacggg gtgacatcac ctcgccgaca
     4561 ttcgtcatct cacggctgca cccgtcgctc gtggacggcc cggcgttgct gcacgtcgac
     4621 gcctaccgtc tcggcgacga ggcggagctc gacgacctcg acctcgacgc cttcgtcgac
     4681 ggttcgatca cggtggtcga gtggggcgag ggaatcgccg aggcactgtc cgaggaccgg
     4741 ctgcgcgtcg acatcacgcg gacgcgtggc accgacgcga ccatcggttt gttgactgcg
     4801 ctggacgacg cccggaccgt gtccgtgacc cccgtcggcg cccgctgggt cggcgccgcc
     4861 gtccgttccc acctctccgc cttcgttgaa ccgtgagttg tggttcgtcc aaccgtgagt
     4921 tggggttccg tcactaggct gggcgggtca tgttgctcgc gttcgacacc gccacccatg
     4981 cggtgaccgt ggcgctgcac acaggtacgc cggatggcga ggtgctcgcc gagtcgaccg
     5041 tcgtggacgc cctacgacac ggcgagctgc ttgcgccagg gatccggcag gtcctggcgg
     5101 atgccggttg tacgccgcgt gacctcaccg ggatcgcggt cggcgtcgga cccggcccgt
     5161 tcacgggtct gcgggtcggc atcgtgaccg cgcggacgat gggtgcagca ctcggcatac
     5221 cggtgcacgg cgtgtgcacg ctcgacgtca tcgccgccgc ggtacgtgat cgggacgacg
     5281 ggttccgggt ggccacggac gcgcggcgca aggaggtcta ctgggccgcg tacgacgcga
     5341 ccggcaagcg cagcacggtg ccttcggtcg accggcccga agacgtgccg tacgacggtc
     5401 cggtggcggg acggggggcg cggctgtact ccgaatcctt cccgcagccg atcgagccgg
     5461 agaaccccga cgcggccgac ctcgccaccc tgatcgcctc cgacgccgtc gaggcgctgg
     5521 cgccggaacc cctgtacctg cgtcgaccgg acgccgcagc gcccggacag cggaagcggg
     5581 tgctgtgaag atccgaccgg cgaccgagca cgacctcgac gccgtggtcg tgctcgagct
     5641 cgaagtcttc ggctctgccg cctggtcgcc acgccaggtc gaggaggagt tcagcgggct
     5701 cggcgacacc cgggcgatct ggctggccga aaccgcaggt gagcacgggg ggttgacccc
     5761 cgtcggatac gccgccggac gtttcgtcgc cgacgtggcg gacgtccagc gggtggcagt
     5821 gctccgtagc cggcgtcgac acggtgtcgg gcagcagctg ctggacgagg tcgtggcgga
     5881 ggcgcaacgc cgcggctgcg atcggctcat gctcgaggtg gccgacgaca acgccgccgc
     5941 gctggcgctg tacggagcca acggcttcac cgagatggac cggcgaccgc agtactacgc
     6001 gaacggtgcc gacgcgctga tcctggggcg gcccgtcgat ggctgacctt ccgctcgtgc
     6061 tcgggatcga gacgtcatgc gacgagacag gcgtcggcct cgtgcgcggc aacgacctcc
     6121 tcgccgacgc cgtcgcctcc agcgtcgacg agcacgctcg gttcggcggc gtcgtgcccg
     6181 aggtcgcgag ccgcgcacac ctggaggcaa tggtgccgac gatcgaacga tcctgtcgag
     6241 acgccagggt ctcgttgtcc gacgtggacg cgatagcggt gacgtccgga cctggactcg
     6301 ccggcgccct cctcgtcggc gtcgcggcgg cgaaggcgct cgcgctgacg ctcgacgttc
     6361 cgctgtacgg cgtcaaccac ctctccgcgc atgtcgccgt cgaccagctc gagcacggcc
     6421 cgctgcccga gccctgcgtc gcgctgctcg tcagcggtgg tcactcgagc ctgctgctgg
     6481 tcgacgacgt gacggccggc gtcgaaccgc tgggcgccac ggtcgacgac gcggccggcg
     6541 aagccttcga caaggtggcc cggctgttgg gcctgccctt cccgggcgga ccgcacatcg
     6601 accgggcggc tcgtgacggc gaggtcgtca tcgactttcc gcgcggtctg tccagcggcc
     6661 gcgacctcga gcggcatcgc ttcgacttct cgttctccgg gttgaagacg gcggtcgcac
     6721 gctgggtcga ggcgcgtgag ctgtccggcg agccggtgcc ggtggctgac gtcgccgcgt
     6781 ccttccagga ggcagtctgc gacgtcctca cccgcaaggc cgtcgacgcc tgcgttcgct
     6841 cgggagccgc ggacctggtg atcggcggag gagtcgccgc gaactcacgg atccgcgtgc
     6901 tggccgagga acgcgccggt cgtcacggca ttcgggtccg ggtgccacgg cccggtctct
     6961 gcacggacaa cggtgcgatg gtcgccgcgt tgggttcgga gctcgtacgc cgcggccgga
     7021 ccccgtcggc gtacgacatc ccggccgatt cctccatgcc gctgaccaca gtcgtggcct
     7081 gagagcggtc gcatcagggg ttcgcggtga gctggtaggg caccaccagc gatcctgtct
     7141 ccgcaggtcg ggccgccgcg gcgagctggc agttcccgtc ctcgaagcag cgcacgatgg
     7201 ccgactcgcc gtcctggatc gtgatgatgt cgagcgcgtt gtcgccctcg aacgtcgctc
     7261 gcccgaacgt tcccgccgtt gtgaacgcgc ggatcactgt tccgtccttc gcgtcgagga
     7321 tcgcgccgct gcgaacgtcg tcgcccgctg cgctggcgct caccgcgaag acccgcgaac
     7381 catccggcga gaactcggtc ggccgccact ggcagctccg ccacagctcg gtcgcgccgt
     7441 cggtcgacag catcgcgctg cattgatgct cgccctcgcc catgtccgtg gtgcgtccga
     7501 ccatcagccc gacggcctgg tctgccgcgg acagcgagac gacgttcggc cagggctgct
     7561 ggaccgaccc aggtcccgaa gaggcgaggt cggccgaccc gacgacctgc gtgccgtccg
     7621 gcgtggccgc gttgaacagg accacgcggt cctggacgtc catcacctgc ttgacgtcga
     7681 gctggtgcgt gtaggcgtag ctcggctggg ccgagagcgc gtcggcatac agcacggtgc
     7741 ctgtctgcgc aggacgtccg tcgacgtcga cgccgttcac ggcgaaggcg acggcgccgt
     7801 tgtggtcgat cgcaggactc gtcgctgccc tggcagaggg gagcggcgtt gcccgggtcg
     7861 ccctgaactc cagccgttgg tcgctgtcgg ccacgaagcc gccgacaccg tccgagaacg
     7921 tagcagcgtc gacgaccgag tgcttcgagc ccgagcctgt ggtggcgatg gccgacaggt
     7981 cggagtgcct gcccgcatgg acgaactgcg cgtcgacgat gtacggcacg gccggagccg
     8041 gacccaacgc gaggccggcc agcgcgatgt cggtgaccgg tgccaccggc gatgtggcgg
     8101 cgcgggatgg cgccggtccg tcggcgacat ccgcagagcg cgtgtcgtcg acgatagtcg
     8161 ccgcgaccgg aacgcagacc gcgaccaccg cggccgcggc cgcggtcacc gcgagacgtc
     8221 ggcgcagcct gaatcgccgc gctcgtcgca gcgacgactc ggtcaggtcg aggtggggtg
     8281 catcgcctgc atggcgctgc agcgtctgcc gaacgtcggc ctcgaggtcg gccccggacc
     8341 cgttgttcgg gggctgcatg tcggtcatgg ctcatgtccc aacaggccgg ggtcggccgt
     8401 caccgccgct ctcatcgtcg agatggcgcg tgacgtctgg ctcttcacgt tgccaaccga
     8461 gcatcccagg atggctgccg tgtcggcctc gctgaggtct tcgtagtagc gcaggacgac
     8521 ggcggcgcgc tgcttgggcg gcagctgctg caccacgtgc cacagggcgt cgcgatcctc
     8581 ggcggcgaga tcgtgcaccg cggactcggg cagttcgccg gcggatcgct cctggcgtcg
     8641 ccaggcacgc cgccacaggc tggcgttctc gttgaggatg atgcggcgga cgtacgcgtc
     8701 caacgcgcca cgatcacgca gcttgttcca tgacaggtac gtcttggcga gcgccgtctg
     8761 gacgagatct tcggcagtgt gatggtccga cgtcagcagg taggcgaagc ggagcagcgc
     8821 atgctgacgg gctcggacga actccacgaa gtcggcctgt gactggtcga ccccgccccg
     8881 gctcacggca cgccacacta actcgccggt cggtgccgtc gtcagcgcgc tcatggctca
     8941 ctcaccctcg ccatcgcttg cgggacgcat cgggtcggcc cggaggttgc atgccggcgg
     9001 tcacgctcgt cgagaaggtc atcccgacag agccaggtgg tgacgcgcaa ggacaaagat
     9061 gtgccggtgt gcgccgaatg ttccgtgttt cgcacggtag atgacccgga acgagtgagt
     9121 gcccacctca cggacacgga accgacggct gtctggcgtg cagccgaagt cccagaggtg
     9181 accagggttt ccactcacgc gcgcccggtg ccacgagtgc tcgacgtagt ctccgacgct
     9241 gcctgacaca cgccgacgac gttcggcacg catcggcgcg gcatcggtga gcaggacgag
     9301 ccgctgatcc gcactgggtg acacccagac gcagcggtcg acgcgatgaa cggtccgcgt
     9361 ccacgctctg ggcacccgcg tggcgacctc gccgtgcatg tccgctccgc acctgacccg
     9421 gtgcgaactg ctgctggacg tctgctccga tctgagggcg gcgtcggctc cgatggcgat
     9481 cgatgacgcc gacgacaccg acaacaccga cgaggtggcg agcaacgcga agccgatgcc
     9541 gagtgctgtc gtggtgagga tccgtctcat gactccccct gcctccggtg tgctgacacg
     9601 caccagacgc gaccagcccc ggacgaagtt gcagcgggcc gacagcgggt gtcgcacgac
     9661 gagatctcag cggcgtcgat cgacgagcaa cgcaacagcg gagtgcggcg tgcgggtcag
     9721 caccagcgtg gccgggcgcg tgcccgagag cgccagccgc gtccgcaagg cctccggagt
     9781 gacacgtacg ccgcgcttct tgatcgtgag cggaccgacg tcgcgcgtac gaagagcggc
     9841 acgcagctgt ctctccttga acggcagcac gtcgagcacc tcgtacgccg ccgcgaacgg
     9901 cgactggacc agccgatcgg cagacacgta ggccacgtgc tcgtccagca gccagccgtc
     9961 gaccagggcg cacaccgtgg tcaccagatg ggcccggatc acggcatcgt caggttcgta
    10021 gacgtagcgg ccgaccgagc ggaccaccgg gtcggcggcc gtgtcccggt cggtcagcgt
    10081 cgcaggtggc acaccactcc gcagcagcgt cgctcgtgcc ctgccgtgcg gtgcgaaccc
    10141 ggaccacagg gtcgcctcct tcagctgtcc gtcgagactc acccactccg tctcgacgtc
    10201 atccggaacg atcgcgtgcg ggataccggg cgccaccttg acgacggccc gtcgactgag
    10261 cagatcttcg acgaacgacc atggcggtgc gtaggagtgc ggatcgaacg tgcgcccgga
    10321 cgcggatcgt cgactggggt cggcgaaggc gacgtcgaac aggaggtggt ccacagcggt
    10381 cacgtcgccg acctccacat gtccggggag cccgaacgac gacaggttgg cggcggccac
    10441 cgccgcagtc gctcggtctc gctcgaaccc gaccacccgg aagccgtgac gtgcgaagct
    10501 gacgaggtcg gagccgatgc cgcagccgag gtcgaccagg gcggcatcgg gcgcgtcgac
    10561 cagggccgcg aggcggacgg cccggtagtc cgccaccgct ggatgggtcg cctgctccag
    10621 accgtcgggc gtgaagtaca tgcgggcggc ctggtcaccg aacttcgcgc tcgctcggcg
    10681 ccgaagctcg acctgggtga gggccgcgct gacatgacct gcgtcgtact cgcggcggag
    10741 tgcgcgggcg agcgccagct cgttatgacc ctcggcccct tcgtcgtacg ccgcctccgt
    10801 ccggcggagc agcgcctgtc ccgccggcgt cagcaggaag ccgaaggcga ccgggtccac
    10861 ccgtgcatgg tggcagaccg acccccggcg ttggcactcg tttggggcga gtgctagatt
    10921 cagcgttggc actctccctg tgagtgtgcc aacgagaagt cacggcaccc gcgacgacgt
    10981 gaactcctcg tcaaggttca tatccgaaag caatcgcctc tctgttcatg agtgaaaggt
    11041 gaggtccagc acgtgtctgt cagcatcaag ccgctcgaag accgcattct cgtccgcacg
    11101 ctcgaagccg aggagaccac tgcctcgggt ctggtcatcc ccgacaccgc caaggagaag
    11161 ccccaggagg gcgaggtcct ggccgtcgga ccgggccgta tcgacgacag cggcaaccgc
    11221 gtcccgctcg acatctcggt cggcgacaag gtgatctaca gcaagtacgg cggcaccgag
    11281 gtcaagtact cgggcgagga ttacctcatc ctctccgcgc gggacgtcct cgccgtcatc
    11341 gagaagtagg ggcgcgactc catctcgctc ctgccgcatt ctcgtggcac gtacttcagg
    11401 cgcgccccgg cggtccgttc gcagacctcc ggggcgcggc cgttcgcagc ggcgcgaacc
    11461 ccctgttccc gcccacccct ctctcgtgag aaggaccctt catgccaaag attcttgagt
    11521 tcgacgagaa cgcccggcgt gcactcgagc gcggcgtcga ctcactcgcc aacaccgtca
    11581 aggtgacgct cggcccgcgg ggccggtacg tcgtcctgga caagaagtgg ggcgcaccca
    11641 cgatcacgaa cgacggcgtg accgtcgctc gtgaggtcga gctcgacgac ccgttcgaga
    11701 acctcggcgc ccagctggcc aaggaggtcg ccaccaagac caacgacgtc gcgggcgacg
    11761 gtacgacgac tgcgaccgtc atcgcccagg cgatggtgca cgaaggtctg cgcgctgtgg
    11821 ccgccggctc caacccgatg agcctcaagc gcggcatcga caaggcggtc gaggccatca
    11881 acgccgagct cggcaacgtc tcccgcgaag tggactccac cgaggacatg gcgcacgtcg
    11941 cgacgatctc ggcccgcgac tcgcgcatcg gtgagctcat cgcgcaggct ttcgacaagg
    12001 tcggcaagga cggcgtcatc actgtcgagg agtccaacac cctcgggacc gagctcgagt
    12061 tcaccgaggg catgcagttc gacaagggct acatctcgcc gtacttcgtc accgacgcgg
    12121 agcggatgga ggcggtcatc gaggacccct acattctcgt caaccagggc aagatctccg
    12181 cgatcaacga cctgcttccg ctgctggaga aggtcatcca ggccggcaag ccgctcttca
    12241 tcatcgccga ggacgtcgac ggtgaggcgt tgtcgacgct ggtcgtgaac aagatccgcg
    12301 gcacgttcca tgcagccgcg gtgaaggcgc cggcgttcgg tgaccggcgc aaggcgatgc
    12361 tccaggacat cgcgacgctc accggtgcgc aggtcgtgtc gcccgacgtc gggctcaagc
    12421 tcgaccaggt cggcctcgac gtgctcggca ccgcccgccg gattgtcatc agcaaggacg
    12481 acaccacgat cgtcgacggc agcggcgacg ctgccgaggt cgacgcgcgg gtggcccaga
    12541 tcaagaacga gatcgagaac accgactccg actgggaccg cgagaagctc caggagcggc
    12601 tcgcgaagct cgccggcgga gtctgcgtcg tcaaggtcgg cgcggccacc gaggtggagc
    12661 tgaaggagaa gaagcaccgc atcgaggacg cggtctcggc caccagggca gccatcgagg
    12721 agggcatcgt cgccggtggc ggctccgcgc tggtgcactc ggccgccatt ctcggcgaca
    12781 acctcggcct gaccggcgac gatgcggtcg gcgtacggat cgtgcgccgt gcgctcgacg
    12841 aaccgctgcg ctggatcgcc gagaatgccg gagaccaggg ttatgtcgtc gtctcgaagg
    12901 tgcgcgagat cggggccggc aacggtttca acgccgcgac gttcgagtac ggcgacctgg
    12961 tctcgcaggg cgtgctcgac ccggtcaagg tgactcgctc ggcgctgtcc aacgccgctt
    13021 ccatcgcggg catgctgctc accaccgaga cgctcgtggt cgagaagccc gaagaggcgg
    13081 agccagaggc cggcggtcac ggccacggtc acggtcacgg ccactgaccc cgaccgccgg
    13141 actggccgcc acccgctgat cgagcttgtc gggatcgagg ggtctcgaca agctcgaccg
    13201 ccggaccctc gggccgcgcc tgctaccggt gcattcctca cgcgtcaagg ttcccttcgg
    13261 gatcgctgac gtcggtgatg gtttgcctgg gtagccatcc aaacggacgg gtagccaggc
    13321 aggtctgcct tgggagggat ggatttggct ggttagccat gcaaaccgcc gccggcgacc
    13381 gaccgatgtc cgaaacccgc cagtcacgca ccgaccaaga tggacggaac ggcggctggc
    13441 cagctcaggt ggcagcaaca cctcccgttc atcgctgccg acccacctca gccccatgac
    13501 aggagatccc gggcgaccac gcccgcccgt cgggaccatc cccgcccccc agaatcccgc
    13561 ggcccccggt cggtaaggac ctctacccgg ccgtgcaccc tacggatcgc gtcgcctgac
    13621 ccgctgcgaa cgacggattc cgtcgtactc gaacgccaac gtgttggccg accccgcgcg
    13681 cgtgcaaggg tttctcctgt gatgacgccg agggctggct cgtcgccgat tgccagcatc
    13741 gcacctccca ccgatctcgc cggcttggcg tgtggggccc agtgcgggga caaggttctc
    13801 gtggaggccc tgctcacccg ggtccgcctc atcgcgcatc gctattcgcg gtcgcgactg
    13861 cttcacttcc ccggtggtca gcagctggcc gacgatgtgg cgcaggacat ctgcctcgca
    13921 gtgctgcggg cgctgccgcg atatcgcgac cagggccgtc cgttcgaagc gttcgtgtac
    13981 ggcgtcgcct cgcgcaaggt cgccgatgcc gttcgcgcgc tgtcccggca tccgcaaccg
    14041 atcgacgatc tccccgacga ggtcgacagc gcgccgactc cggaggacag cgccgttcac
    14101 cgcagcgagg tcgagtcggc ggcccggctg ctcgcggccc tgccgaagac gctgcgcgag
    14161 gtcatgctgc tgcgcgtcgc gaccggtctc acggccgagg agaccggagc ggcgctgggg
    14221 atgaccgccg gcgcagttcg ggtcgcccag caccgagcac tggcgaagat gcgcgagcac
    14281 gccaaacggc tcgatcgcat ccctgcgaac tgcgggagca gtcaagccga agcgccgtag
    14341 cgcagctgac ccacgtcagg cttccgggag acgcggtccc cgtaccattg gggcatggat
    14401 ctgacctcgg cgggcgttcc cgacaagttc gcgactctcg ggctcacgtt cgacgacgtg
    14461 ctgctcctcc ccggtgagac cgacgtggtt ccgagcgaga tcgacacgac cacgcggctg
    14521 acccgcgaca tctccgtcaa gatcccgctc gtctcgagcg cgatggacac cgtcaccgag
    14581 tcccggatgg cggtcgcgat ggctcgccag ggcggtctcg gcgtactgca tcgcaacctc
    14641 tcggtcgacg agcaggccgg gcaggtcgac ctcgtcaagc ggtcggagtc gggcatgatc
    14701 accaacccgg tgacggtcgg gcccgacgtc acgcttgacg acctcgacga gctgtgcgcg
    14761 cgctatcggg tctccggtct tcccgtcgtc gacgacgagg gtgtcctgct gggcatcatc
    14821 accaaccgcg acctcaggtt cgtcccgatg agcgagtggt cgaccagacg ggtccgagag
    14881 gtgatgacac cgatgccgct cgtcaccgcg ccggccggga tcagctccga ggacgccgcc
    14941 gcactgctcg ccaagcacaa ggtcgagaag ctacccctgg tcgacgagaa cgaccggctg
    15001 tccgggctca tcacggtcaa ggacttcgtg aagtccgagc agtacccgaa cgccacgaag
    15061 gaccaggacg gtcgcctgat ggtgggcgct gccatcgggt acttcggcga cgcctgggat
    15121 cgtgccgcag cgttggtcga ggcgggcgtg gatgtcctgg tcgccgacac cgcccacggc
    15181 cacgtccggg tgatgctcga catggtgcgc cggctcaaga ccgacgcggc gacccggcat
    15241 gtccaggtga tcggcggcaa cgtggcaact cgcgccggcg cccaggcgtt cgtcgacgcc
    15301 ggcgcggatg gcgtcaaggt gggcgtcggg cctggctcga tctgtacgac gcggatcgtc
    15361 gccggggtag gcgcgccaca ggtgaccgcg atccacgaag catcgctggc ctgtcggccg
    15421 gcaggcgtgc ccgtcatcgg tgacggtggt ctgcagtact ccggcgacat cgcgaaggcc
    15481 ctcgtggccg gtgccgacac ggtgatgctc ggctcactgc tcgcggggtg cgaggagagc
    15541 ccgggcgacc tggtgttcgt caacggcaag cagttcaaga gctatcgcgg gatggggtcg
    15601 ctcggcgcga tgtcgtcacg cggccggaag tcgttctcca aggaccgcta cttccaggcc
    15661 gaggtcacca gcgacgacaa gctggtgccg gagggggtcg agggccaggt cccgtatcgc
    15721 ggcccgctcg ccgcagtggc ccaccaactg gtcggtgggc tgcaccagtc catgttctac
    15781 gtcggggccc gcaccatcgg tgagctccag gagcgcggtc gcttcacccg catcactgca
    15841 gccggcctct ccgagagcca tccgcacgac atcgagatga ccgtcgaggc tccgaactac
    15901 tcgggtcgct gaggatggac atcgagatcg gtcgcgccaa acgcggtcgc cgcgcctacg
    15961 cgttcgacga catcgcgatc gtgcccagcc ggcgaacgcg cgaccccgag gaggtctcgg
    16021 tcgcctggca gatcgacgcg taccgcttcg agatcccgat cctggccgcg cccatggact
    16081 cggtcatgtc gccggaaacc gcgatcgcct tcggcaaggc cggcggcctc ggggtgctcg
    16141 acctcgaagg gctgtggacc cggtacgacg acccaacgcc gttgctggcc gacgtggcgt
    16201 cactgcgcgg gcacgaagcg accagccgcc tccagcagat ctatgccgaa ccgatcaagc
    16261 cggagctcat ggtcgagcgg gtcaagcaga tgcgtgactc cggcatcacc gtcgccggag
    16321 cgctgtcgcc gcaacggacc aaggagcacg cccagtccgt gatcgacgca ggcttggacc
    16381 tgttcgtcat ccgcggcacc acagtctcgg ccgagcacgt gtccgggcag gcggagcccc
    16441 tgaacctgaa gcagttcatc tacgagctcg acgtccctgt catcgtcggt gggtgtgcga
    16501 cgtaccaggc cgcgctccac ctgatgcgga ccggcgctgc cggagtactc gtcgggttcg
    16561 gcggcggcgc agcgcacacc acgcggaccg tgctcggtct ggcggtgccg atggccaccg
    16621 cggtggccga cgtggcggcg gcacgacgtg actacctcga cgagtcgggc ggccgctacg
    16681 tgcatgtgat cgcagacggt tcgatcggca agtccggcga catcgcgaag gcgatcgcct
    16741 gcggcgccga cgccgtgatg atcggctcac ctctcgctcg ggccgaggag gcgccgggcg
    16801 gcggctacca ctggggcgcg gaggcgcggc acagcacact cccgcggggt gagcgcgtgg
    16861 agatcggcac gatcggcacc ctcgaccaga tcctgcacgg tccgtcgctg gtgtccgacg
    16921 ggaccatgaa cctggtcggt gcactgaagc gatcgatggc caccacgggc tacaccgagc
    16981 tcaaggagtt tcagcgcgtc gaagtcacgg tcgaatgaac ctgtccgtgc tacgggtcgc
    17041 ggccgtccag gcggagtcgc gggcgggcga cgtccggcac aacgtcgcga tcgctgcccg
    17101 ttgggtggcg aaggctgccg ccgaagatgc cccgctcgtg gtccttcccg aactgttcct
    17161 gcccggctac gacccgaaga ccttgcgcga ggaccccgac ggctgcgacg tgacggtcga
    17221 cgacccacgg ttggggcctt tgtcggaggc ggccgcttcg gcgggtgtca cgctcgtcgt
    17281 cggcgcgtcg gtgcgtgagg gtgagcgtcg gctgctgagc gccctcgtct tctcgaccac
    17341 cggtgacata gcggccacgt atgcgaagca gcacctgtgg gacgcggagc ggggcatctt
    17401 cacgcctggc acacacggca cgtccctggt cttccgtggc tgggacctcg gactcggcat
    17461 ctgctacgac ggctgcttcc ccgagcatgc ccgcgcggcg accgatgcgg gtgcgctggt
    17521 ctacgtgtgc ccgtcggcgt acgtggtggg cagcgagcac cgccgggacg tctactacgc
    17581 cgcccgcgcg atcgacaacg gcatctacgt cgtcttcgcc ggactggtcg gaccgtgcgg
    17641 cgagctggag ttctccggag gtacggcggt ctacgacccg cagggccgta ccctctcccg
    17701 ggtggagtcc ggcgagggga tggtcgtcgt cgacctcgac cccgaggcga tcgcagaagc
    17761 gcgtcgcatc aacccgtatg agcgcgaccg cgtctcggac accgagccac gccacatcct
    17821 gcccctctga cggcacctga cccgccgcag cagctaccgc ggattgcggg aaacgtacgc
    17881 cctacggccc ccgaagagcc tgcactttcc cgcaattcgc agcgacccaa tgcgaccttc
    17941 ggcctgttgc gtcgtacggc catgcggcaa tagcgtcgag tggtgccgcg ggaccctgat
    18001 gtcggagtgg acggacctgc atctgaggcg ctgctgaagt tgggccggtt cttcacgagg
    18061 tgggacgagt ccgaggacgg gcgcgcagtc ttccgcgagg gtggtcgcgc cggcgatgtc
    18121 ttctaccgtg accggtggag ccacgacaag gtcgtgcgat cgacgcacgg cgtgaactgc
    18181 accggctcgt gctcctggaa ggtgtacgtc aaggacggga tcatcacctg ggagacgcaa
    18241 cagaccgatt acccgtcggt ggggccggac cggccggagt acgaaccgcg cggctgccca
    18301 cggggggcgg cgttctcctg gtacacctac tccccgaccc gggtgcgctt cccttatgtg
    18361 cgcggcgtac tgctcgacat gtaccgcgag gctcgcaacc ggctcggtga cccggtggtc
    18421 gcctgggccg agatcaccgg tgactccgag cgccggcggc gctaccaaca ggcccgcggc
    18481 aagggcggtc tcgtgcgggc ctcgtggcgg gaggcggtcg agatcgcggc cgccgcccac
    18541 gtccacacga tcaagaccta cggcccggac cgatgcgccg gcttctcccc gatcccggcg
    18601 atgtcgatgg tctcgcactg cgtgggaacg cgtttcatcc agctcatcgg cggcgtcatg
    18661 acgtcgttct acgactggta cgccgatctg ccggtcgcga gcccgcaggt cttcggcgac
    18721 cagaccgacg taccggagtc
//
LOCUS       DATBFZ010000093         5994 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_5935139, whole genome shotgun sequence.
ACCESSION   DATBFZ010000093 DATBFZ010000000
VERSION     DATBFZ010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 5994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5994
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5935139"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            10..135
                     /locus_tag="VH419_02765"
     CDS             10..135
                     /locus_tag="VH419_02765"
                     /inference="COORDINATES: protein motif:HMM:NF016840.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042571.1"
                     /translation="MEEYGRHTGQADLIRESVDGLVGEDPPPGWRPQVGRSPVGA"
     gene            complement(255..2918)
                     /locus_tag="VH419_02770"
     CDS             complement(255..2918)
                     /locus_tag="VH419_02770"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEY7042572.1"
                     /translation="MAEPPWLALPLLAVALTAAGHLRVRFRRGEDVDSITLFEAVLAP
                     LIFSFPTPAVVGTVVVAQVATSLLRRTTLVKAGFNVAQWALAAAVGSVVLNSLRPDDQ
                     VTLQSMAEVVLGLACIAIVNNSAFTVVLAISGQQSVRSVLRDMRPVLVPGWLVGWAVN
                     TMIGLLFVMAFASTPAAVILFPVPLVVLHLAYRGYAAARSDRFRLAGLRAAAQTLAAP
                     LQPEEAFDDFLVDVTRCFEAGGAALLLLTETGELDVHEYDRTGNAQTTPEVDDAQPLA
                     AHLSELSRPASFSASDQHPAGELLRRFGWRDCICAPLVDEHRRLGTLVVFNRTGLEGD
                     TQADLAILETMARETAHTIARGLLFESVVEERRKLDEIVGTTSDGIFTLTDDGTVLTW
                     NAGCENITGLGADGVLGRSDALVRLRARTTKGVPIDVAAWVGNPNVPADLVITRPDGT
                     HRRLSCSVSTANDVDLLTTTHVVVARDITPGEEYEALREQFSRLVEAQAAQRLVVEHL
                     QRAVAPEPPGIEGGDIAVAYVASDASSPTGGDLFDWHPLPNGELHVAVVDLLGHGVEA
                     TKHALLVVHALRFAAMDGTPLDEMVLRADRLLSAQSSEIVATVVIARYRPETGELKVV
                     SGGHPPALVVRADGEVMQLTASGGAIGWPGVGSDNVVTTTLQPNESLVLYTDGLIEAR
                     KNVIDGMDSLIGHASDIAHLPVAEFADELVKRSLEGAHRRDDSLALVLRRTRIPASPS
                     RMSWDVEPGSQRSIRLARQGLEKWLVARRAQTDDAVLVAAELMANAVVAARSSVVLSA
                     AQDGDNLVLEVADDGQGDAHLDELGEVLPITESEHGRGLFLVRALSDDVAMMSTAEGT
                     VVHCVLPMGAASQGSAAPGSTSSSTQDVSNR"
     gene            complement(3034..3285)
                     /locus_tag="VH419_02775"
     CDS             complement(3034..3285)
                     /locus_tag="VH419_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042573.1"
                     /translation="MKQRLIAIAATTFAAAILWSGAGTAPALAGNDWEFTPDSTQSST
                     TGGTASPDGNDWEFAAAKSPALGHLGVITSRMALGNDWE"
     gene            complement(3506..4000)
                     /locus_tag="VH419_02780"
     CDS             complement(3506..4000)
                     /locus_tag="VH419_02780"
                     /inference="COORDINATES: protein motif:HMM:NF003819.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HEY7042574.1"
                     /translation="MAGDSSFDIVSKIDHQEVDNALNQAAKEISQRFDFKNTGAAISW
                     RGESSITVTANADERALAVIDVFREKLIKRHQSLKILDVSDPRASGKEVKVDIDLKEG
                     ISQEQAKKVSRLIRDEGPKGVKVQVQGDELRVSSKKRDDLQTVISLVKEQDYDFAIQF
                     TNYR"
     gene            4125..4206
                     /locus_tag="VH419_02785"
     tRNA            4125..4206
                     /locus_tag="VH419_02785"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4159..4161,aa:Tyr,seq:gta)
     gene            complement(4315..5163)
                     /locus_tag="VH419_02790"
     CDS             complement(4315..5163)
                     /locus_tag="VH419_02790"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020386240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEY7042575.1"
                     /translation="MTIPSTTGPVDGERPVRVGAQLQPQHAEYADIRRAVAEAEEIGV
                     DVVFTWDHFFPLYGDPDGKHFECWTILGAWAEATEHVEIGALVTCNSYRNPDLLADMA
                     RTVDHISGGRLILGIGAGWCERDYASYGYEFGTPGRRLDALGEALPRIKKRFHALNPA
                     PTREIPVLVGGGGERKTLRHTATYADIWHGFGNVETITHKHQVLDTWCRDVGRNPREI
                     ERSAKINNGDLDFAPGLYAAGTRLFTMSATGPAYDLGELRELVAWRDAVNRGQIRSTE
                     PPGPVR"
     gene            5298..5370
                     /locus_tag="VH419_02795"
     tRNA            5298..5370
                     /locus_tag="VH419_02795"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5331..5333,aa:Thr,seq:ggt)
     gene            5451..5527
                     /locus_tag="VH419_02800"
     tRNA            5451..5527
                     /locus_tag="VH419_02800"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5485..5487,aa:Met,seq:cat)
     gene            5592..5762
                     /gene="rpmG"
                     /locus_tag="VH419_02805"
     CDS             5592..5762
                     /gene="rpmG"
                     /locus_tag="VH419_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEY7042576.1"
                     /translation="MASKSTDIRPKITMACTECKERNYITRKNRRNDPDRMELKKYCP
                     RCRKHQPHRETR"
BASE COUNT          964 a         2001 c         2045 g          984 t
ORIGIN      
        1 tgcgacctga tcgaggagta cggccgtcac accgggcagg cggatctgat ccgggagtcg
       61 gtggacggac tggtgggtga ggatcctccg cccggctggc gcccgcaggt cgggcgatca
      121 ccggtcggcg cctgacgggt acgatcagcg catgctggta ctcatcatcg tggtcatcgc
      181 cgtcatcgtg atcggcggcg tcggagtggg ctggcgccga cgcggtcgtt gagaccgtcg
      241 actcctgcgg agcctcagcg gttgctcacg tcctgcgttg acgaactcgt cgagccagga
      301 gccgcggagc cctgcgacgc ggcgcccatc ggcaggacac agtggacgac cgtcccctcg
      361 gcggtgctca tcattgcgac gtcgtcgctg agagcacgaa cgaggaacag gccacgtccg
      421 tgctcgctct cggtgatcgg cagtacttcg ccgagctcgt cgaggtgagc gtcgccttga
      481 ccgtcatcgg cgacctcgag caccaggttg tccccgtcct gcgccgcact caacacgacg
      541 ctcgaccggg cggccacgac ggcattcgcc atcagctcgg cagccacgag gacggcgtcg
      601 tcggtctgcg cgcgtcgcgc gaccaaccac ttctccagcc cctgtcgtgc cagtcggatg
      661 gagcgctggc tgccgggctc gacgtcccag ctcatccggc tcgggcttgc cggtatccgc
      721 gtgcgccgca gcaccaacgc caggctgtcg tcgcgacggt gggcgccctc gagtgaccgc
      781 ttgacgagtt cgtcggcgaa ctccgcaacc ggcaggtgag cgatgtcgct cgcatgaccg
      841 atcagtgagt ccatcccgtc gatgacgttc ttgcgggcct cgatcagccc gtctgtgtag
      901 aggacaagcg actcgttggg ctgcagcgtc gtcgtgacga cgttgtcgct ccccacacct
      961 ggccagccga tcgcgccgcc gctcgcggtc agctgcatga cctcgccgtc cgcgcgtacg
     1021 acgagagctg gcggatgtcc tcccgagaca accttgagct cgccggtctc ggggcggtag
     1081 cgcgcaatga ccaccgtggc gacgatctcc gacgactggg cggacagcaa ccggtcggcg
     1141 cgcagcacca tctcgtccag cggtgtgccg tccatggccg cgaagcgcag ggcgtgtacg
     1201 accagcagcg cgtgtttggt ggcttccacg ccatggccga gcaggtcgac caccgcgacg
     1261 tgcagctcac cgttgggcag cggatgccag tcgaagaggt caccgccggt cggcgacgac
     1321 gcgtccgagg cgacgtaggc gaccgcgatg tcaccacctt cgatgccggg gggctcgggt
     1381 gccacggcac gttgcaggtg ctccacgacg agccgttgtg ccgcctgcgc ctcgaccagt
     1441 cggctgaact gttcgcgcag ggcttcgtac tcctcacccg gggtgatgtc acgcgccacc
     1501 acgacgtgcg tggtcgtcag caggtcgacg tcgttggcgg tgctcaccga gcaggagagc
     1561 cgacgatgcg tcccgtccgg tcgggtgatc acgagatctg ccggcacgtt cgggttgccg
     1621 acccatgccg cgacgtcgat cggcactccc ttggtcgttc gtgcgcgcag tcgtaccagg
     1681 gcgtctgatc gtcccagtac gccgtcggcg ccgaggccgg tgatgttctc gcaaccggcg
     1741 ttccaggtga ggaccgtccc gtcgtcggtc agcgtgaaga tgccgtccga cgtcgtaccg
     1801 acgatctcgt ccagcttgcg acgctcctcc acgacgctct cgaagagcag gccgcgggcg
     1861 atcgtgtgtg ctgtctcgcg agccatcgtc tcgagaatcg cgagatcggc ctgtgtgtcg
     1921 ccttccagac cggtgcggtt gaacacgacc agcgtcccca gtcggcggtg ctcgtcgacg
     1981 agcggggcgc agatgcagtc gcgccagccg aaccgccgca acagctcacc tgccgggtgc
     2041 tgatccgacg cggagaagct ggccggtctg gacagctcgc tgaggtgggc cgcgagaggc
     2101 tgcgcgtcat ccacctccgg ggtcgtctgc gcgttgccgg tgcggtcgta ctcgtggacg
     2161 tcgagctcac cggtctcggt gagcaggagc agagccgcac cgcccgcctc gaagcagcgg
     2221 gtgacgtcca cgaggaagtc gtcgaaggct tcctcgggct gcagcggggc ggcgagagtc
     2281 tgtgcggcag cacgcagacc ggccaaccgg aagcggtcgg acctggcggc ggcgtacccg
     2341 cgataggcca ggtgcagcac gacgagcggc accgggaaca agatgacggc tgccggtgtg
     2401 ctggcgaacg ccatcacgaa gagcaacccg atcatggtgt tgaccgccca ccccaccagc
     2461 cagccgggca ccaagacggg ccgcatgtcg cgcagtacgg agcgcaccga ctgctggccg
     2521 gagatcgcga gcaccaccgt gaaggcgctg ttgttgacga tggcgatgca ggcgagcccg
     2581 agcacgacct ccgccatcga ctgcagcgtt acttggtcgt ccgggcgcag cgagttgagc
     2641 accacactgc cgaccgcggc cgccagcgcc cactgggcga cgttgaaccc agccttgacc
     2701 agggttgttc gccgcagcaa cgacgtcgcc acctgtgcga cgaccaccgt gccgacaacc
     2761 gcgggtgtcg ggaacgagaa gatcaacggc gcgaggacgg cctcgaacag ggtgatcgag
     2821 tcgacgtcct cgccgcgccg gaaccgaact ctgaggtgtc cggcggcggt cagcgcaacc
     2881 gcgagcagcg ggagggccag ccacggcggc tcggccatgg ggctgaggtc gaagaactcg
     2941 ccgatggcga cgacgacgcc cgtggctacg agaagcgcag caacgtagac ggtcagccgg
     3001 cggtcgaccc ggaccgtgcc agccagagct gaactactcc cagtcgttgc ccagcgccat
     3061 ccgggatgtg atcacaccga gatggccgag ggcaggggac ttggcagctg cgaactccca
     3121 gtcgttcccg tcaggggacg cggtgccgcc tgtcgtcgac gactgcgtgg agtcaggtgt
     3181 gaactcccag tcgttcccgg ccagggcggg ggccgtacca gctccggacc acaggatggc
     3241 cgcggcaaag gttgtcgcgg cgatcgcgat caggcgctgc ttcatggcgt ctccagacat
     3301 ttagctcctg acggagcggg gtcgacaacg acgcgacact tggggacatg tgacgcggcg
     3361 tggcatcagt gttgcacatt tccaggcctt gagcacaagt cttcgcggat gagacgaccg
     3421 acgcgctttc atcctcacgg gcagtgatcg gagggtgggg gttgcgtgac cgaaagtccg
     3481 gtttccggcg tgcgacgggg tggaatcagc ggtagttggt gaactggatc gcgaagtcgt
     3541 agtcctgctc tttcaccagc gagatcaccg tctgcaggtc gtcacgcttc ttgctcgaga
     3601 cccgcagctc gtcgccttgg acctggacct tgacgccctt gggcccttcg tcccggatga
     3661 gccgggagac cttcttggcc tgctcctggc tgatgccctc cttgagatcg atgtcgacct
     3721 tcacctcctt gcccgaagca cgagggtcgg agacgtcgag gatcttcagc gactggtgcc
     3781 gcttgatcag cttctccctg aagacgtcga tgactgcgag cgcccgttcg tcggcgttgg
     3841 cagtcacggt gatggaggac tcgccgcgcc agctgatcgc ggcgccggtg ttcttgaagt
     3901 cgaaccgctg cgagatctcc ttggcggcct ggttgagcgc gttgtcgacc tcttggtggt
     3961 cgatcttgct cacgatgtcg aacgaagagt cgcctgccat ggcagaccgc ctttcgcgtc
     4021 gtcggtgcgc cgcgaggacg ctttgcactc caccctggga cagggggtat ccttgctgct
     4081 cgctgcgtgg ccgccggcct cgactgtggt ccgcggcagc acctggcagg ttgcccgagt
     4141 ggccaaaggg agcagactgt aaatctgccg gctcagccta cgtaggttcg aaccctacac
     4201 ctgccaccct ccgcccagtc ctcggactgg gcgtcgtggt tcgcgggcct cgcaaggcgc
     4261 gggcacgccg acgaaatgcc tggtcacgac cgcgtagcgg agcaggcggc ttggctatcg
     4321 gaccggccct ggcggttccg tcgaccggat ctgtccacga ttgaccgcgt cgcgccaggc
     4381 aaccaactcg cgaagctcgc cgaggtcgta cgccgggccg gtggcgctca tggtgaacag
     4441 gcgcgtcccg gcggcgtaca gcccaggtgc gaagtcgagg tcgccgttgt tgatcttggc
     4501 cgaccgctcg atctcgcgcg ggttgcggcc gacgtcgcgg caccacgtgt cgagcacctg
     4561 gtgtttgtgc gtgatggtct cgacgttgcc gaagccgtgc cagatgtcgg cgtatgtcgc
     4621 cgtgtgccga agcgtcttgc gctccccgcc gccgcccacc aggacgggga tctcgcgggt
     4681 cggcgccgga ttgagggcgt ggaaccgctt cttgatgcgc ggcagcgcct cgccgagcgc
     4741 gtccagccgc ctcccgggag tcccgaactc gtagccgtag ctggcgtagt cccgctcgca
     4801 ccagccggcg ccgatcccga gaatgagccg accaccgctg atgtggtcga cggttcgcgc
     4861 catgtcggcc agcaggtcgg ggttgcggta ggagttgcac gtcacgagcg cgccgatctc
     4921 cacgtgttcg gtggcttcgg cccaggcgcc gaggatggtc cagcactcga agtgcttgcc
     4981 gtccgggtcg ccatacagcg ggaagaagtg gtcccaggtg aagacgacgt cgacgccgat
     5041 ctcctcggcc tcggcgacgg cacggcggat atccgcgtac tccgcatgct gcggctgcag
     5101 ctgagcgccg acgcggaccg gccgctcgcc gtcgaccgga ccggtggtgg aggggatggt
     5161 cacgcgatcg agcctagtgc tccgacgcgg gacactgggt ctcgtggccg gctccggcca
     5221 ctgacgtgac cggcgaacag gctggtcgcc acgattaacg ctccccgggc cagcctgtgt
     5281 atcctcgtac gtcgcttgcc ccaatagctc agtcggtaga gcgtctccat ggtaaggaga
     5341 aggtctacgg ttcgattccg tattggggct caggagtcgg agccctcatc cggtcgtcca
     5401 cgcaggcagc agccggccac gatgcccggg tgagcgcccc ggtttcgccc ggcggggtag
     5461 ctcaggtggt cagagcacac ggctcataat cgtggtgtcg cgggttcaag tcccgccccc
     5521 gctaccaagc agacgagcag cacaccggca tgaacagcaa caacccatag atttcgagac
     5581 gaggcaaccc cgtggccagc aagagcaccg acatccgccc gaagatcacg atggcgtgca
     5641 ccgagtgcaa ggagcgcaac tacatcaccc ggaagaaccg gcgcaacgac ccggaccgga
     5701 tggagctcaa gaagtactgc ccccgctgcc gcaagcacca gccgcaccgc gagacccgct
     5761 gacttccgcg cgacgcgcgc ccggacgacg acccgcatct gctcggcagg tgcgggtcgt
     5821 gtcgtgggta gcgtcgcctc catgccgcct ctcgacccgg acctcgccgg gcacaccttc
     5881 gacccgaccc cgccgtacac cgtgagtcgc gagaagatcg ccgagttcac gacggcgatc
     5941 ggggccggtc cgatcgacga cggggagacg gctcccctga cgttcccgat cgtg
//
LOCUS       DATBFZ010000094         5403 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_2351411, whole genome shotgun sequence.
ACCESSION   DATBFZ010000094 DATBFZ010000000
VERSION     DATBFZ010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 5403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5403
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2351411"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(88..1335)
                     /locus_tag="VH419_02810"
     CDS             complement(88..1335)
                     /locus_tag="VH419_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013160596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEY7042577.1"
                     /translation="MRPSRGTRSMRTLVKVAVVGAVATEVVVIGTLMAVDSWRKRVRP
                     RQAQFPRTDPATVEVGGSVATVYTYGDDLYADMLAAIRGARHRILFESYIVKGDRVGA
                     EFKQALCDAAARGVEVYVLYDGFANLVVPRSFFDFPETVNVIRFPTFRPGVLLLNVRK
                     SGRDHRKILVVDGEVGFVGGYNIGALYATQWRDTHVRVTGPAAWELQNAFVDLWNRVR
                     DVHRPRLPDPGTDVWDPRIRAQRNVPVQLVYPIRGMYLEAIDRAQHHIYITQAYFIPD
                     HDILDALLAAAARGVEVAILVPENSNHVLADWMSRGFYSTLLSHGVQLWLYQGAMVHA
                     KTATVDGHWSTVGTANIDRLSLTGNYEVNLEIVDDAFARHMEQVFLTDRSNARLLDED
                     EWLSRPYAAKFSEAILGPLRPLL"
     gene            complement(1353..2108)
                     /locus_tag="VH419_02815"
     CDS             complement(1353..2108)
                     /locus_tag="VH419_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020388811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HEY7042578.1"
                     /translation="MAESDAPRTTDLAGDLPPEQHVDHEHPDVTGGWLRPAVFGAMDG
                     LVSNFALIAGVVGGTNGEASKAVVLAGLAGLAAGAFSMAAGEYTSVASQAEYTEREIE
                     VERLEIIAKPRAEQAELAQMYVDRGIDRDLADKIAAQVHADPELAVEVHAREELGVDP
                     GNLPSPVVAAVSSFVSFAVGASLPVLPYLLGADTLWPALVISLVALFACGAIVSQVTV
                     RTWWFGGIRQMVLGGLAAAITFFIGDLVGASLG"
     gene            complement(2147..3046)
                     /gene="lgt"
                     /locus_tag="VH419_02820"
     CDS             complement(2147..3046)
                     /gene="lgt"
                     /locus_tag="VH419_02820"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEY7042579.1"
                     /translation="MSAITLASIPSPSEGVWHIGPFPVRGYALCIILGVLVAVWVGER
                     RWIARGGLPGAVGDVAIWAVPFGLVGGRLYHVLTDPELYFGEGKSPITALYIWRGGLG
                     IWGAISLGALGAYLGCRRHKLRFAAFADALAPGIALAQAVGRWGNWFNQELYGKPTDL
                     PWGLEIDPAHRGDFQQYSTFHPTFLYESLWDVGVAGLVIWLDRRLKLGHGRAFAVYVM
                     AYTAGRVWIEYLRVDTANHFFGVRLNVWTSIVLFALAAAYFVIVGRRYPERETEVQVI
                     PQELADEPAGDTERPENGGVRST"
     gene            complement(3058..3732)
                     /locus_tag="VH419_02825"
     CDS             complement(3058..3732)
                     /locus_tag="VH419_02825"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEY7042580.1"
                     /translation="MTPPARRARHLTLPLLVTLLCAVVAACGGDPVVMQGSADNSGGV
                     MSSGGDPRFNGAEPATPYRMPDITLTATNGQPFNLITDTAYPVTLVFFGYTHCPDVCP
                     LVMSDVAQTMLQLPGDVAHRTQFVFITTDPARDTPGVLREYLDHYNPDFVGLTGGLGD
                     IVKAADAMGVAIEGRQQLPGGGYDVGHGAQVVGFRGNEAPVVWTDGTPISDMVSDIDT
                     LAESAR"
     gene            complement(3797..4600)
                     /gene="trpA"
                     /locus_tag="VH419_02830"
     CDS             complement(3797..4600)
                     /gene="trpA"
                     /locus_tag="VH419_02830"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009074976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEY7042581.1"
                     /translation="MTGSADAFEAAKADGRAALVGYLPAGFPDVARSIALLRSMVRGG
                     CDVIEVGLPYSDPVMDGPTIQAAADQALRAGTRIGDVIAVVEAVAELVPTLVMTYWNP
                     VERYGVKRFAADLAAAGGSGLITPDLIPEEAAEWVAAADDLDLDKVFLVAPSSTASRL
                     ELTTAACRGFVYATAVMGVTGAREQTSSVTPELVRRTRGVTDIPIGVGLGVSNGEQAA
                     EVASYADGVIVGSAFVRAVVRSDARGEGAENDAVEALAAELSRGVRGEL"
     gene            complement(4597..>5403)
                     /locus_tag="VH419_02835"
     CDS             complement(4597..>5403)
                     /locus_tag="VH419_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEY7042582.1"
                     /translation="EVDTERQALNVARMKMLGAEVIPVTTGSRTLKDAINEALRDWVA
                     NVETTHYLLGTAAGAHPFPAMVRDFARGIGDETRAQSLALLGRLPDAALACVGGGSNA
                     IGMFAGFLGDEDVRLYGFEAGGEGVSTGRHAATISAGEVGVLHGTRSFLLQDEDGQTI
                     ESHSISAGLDYPGVGPEHAWLAETGRATYRPITDAQAMDAMAALARTEGIIPAIESAH
                     ALAGAYDVAAELGADAVLAICLSGRGDKDMSTAATWFDLVDKAPSEGGLA"
BASE COUNT          871 a         1889 c         1836 g          807 t
ORIGIN      
        1 cggctggacg ccgcgtcacc cgagttggcg aacgggattc gcgcaggcgc tgtgacggtg
       61 agggcctgcg ggaccgtggg atgacgttca gagcaacgga cgcaatgggc cgaggatcgc
      121 ctcgctgaac ttcgctgcgt acggccgcga cagccactcg tcctcgtcga gcagtcgggc
      181 gttcgaccgg tcggtcagga acacctgctc catgtgccgt gcgaaggcgt cgtcgacgat
      241 ctcgaggttg acctcgtagt ttccggtcag gctgagccgg tcgatgttcg ctgtcccgac
      301 ggtgctccag tgcccgtcga cggtggccgt cttggcatgg accatggcgc cctgatagag
      361 ccacagctgc acgccatggc tcagcagcgt gctgtagaag ccgcgggaca tccagtcggc
      421 cagcacatgg ttggagttct cggggaccag gatcgcgacc tcgaccccgc gagcggccgc
      481 ggcaagcaac gcatcgagga tgtcgtggtc ggggatgaag tacgcctgcg tgatgtagat
      541 gtggtgctgt gcccggtcga tcgcctccag gtacatgccg cggatcgggt acacgagctg
      601 gacgggaacg ttgcgctgtg cccggatccg cggatcccag acgtccgtac cgggatcggg
      661 gagtcgcggc cggtgcacgt cccgtacccg gttccacagg tccacgaacg cgttctgcag
      721 ctcccaggcg gcgggtccgg tgacccgcac atgggtgtca cgccactggg tcgcgtagag
      781 agcaccgatg ttgtatccgc cgacgaaccc gacttccccg tcgacgacca ggatcttgcg
      841 atggtcacga ccggacttgc ggacgttgag aagcaggacg ccgggtcgga acgtcgggaa
      901 ccgtatgacg ttgaccgtct ccgggaagtc gaagaagctg cgtggcacca cgaggttggc
      961 gaagccgtca tacaggacgt agacctcgac gccgcgagcg gcggcgtcgc acaatgcctg
     1021 cttgaactcc gcgccgacgc ggtcgccctt gacgatgtag gactcgaaca ggatccggtg
     1081 gcgggcaccc ctgatggccg ccaacatgtc ggcgtacagg tcgtcgccgt acgtgtagac
     1141 cgtcgccacc gagccgccga cctcgacggt ggcagggtcc gtccggggga actgcgcctg
     1201 gcggggacgg acccgcttgc gccagctgtc gaccgccatc agcgtgccga tgacgaccac
     1261 ctcagtcgcc acagcgccga cgacggcgac cttcaccagg gtgcgcatcg agcgagtgcc
     1321 gcgcgacggt ctcatgtcag cccagcctcc tgtcagccca gcgaggcgcc gacgagatcg
     1381 ccgatgaaga acgtgatggc agcggccagg ccgccgagga ccatctgccg gatgccaccg
     1441 aaccaccagg tgcgcaccgt cacctgcgac acgatggccc cgcacgcgaa cagggcgacg
     1501 aggctgatga ccagagccgg ccacaacgtg tcggcgccga ggaggtacgg cagcacgggc
     1561 agcgaggctc ccacggcgaa cgacacgaac gacgagacgg ccgccacgac gggagacggc
     1621 aggttgccgg ggtcgacgcc cagctcctca cgggcatgca cctcgaccgc cagctcgggg
     1681 tcggcgtgca cctgcgcggc gatcttgtcg gccaggtcgc ggtcgatgcc ccggtcgacg
     1741 tacatctggg cgagctctgc ctgctccgcg cgcggcttgg cgatgatctc caaccgctcg
     1801 acctcgatct cccgctcggt gtactcagcc tggctggcca ctgacgtgta ctctcccgcg
     1861 gccatcgaga acgctccggc ggcgagcccc gccaggccgg ccagcaccac tgccttggag
     1921 gcctccccgt tcgtgcctcc gacgactccg gcgatcagcg cgaagttcga gacgaggccg
     1981 tccatcgcgc cgaacaccgc cggacgcagc cagccgccgg tgacatcggg gtgctcgtgg
     2041 tcgacgtgct gctcgggagg aagatcaccg gcgaggtccg tcgtgcgcgg ggcgtccgat
     2101 tcggccatgg tcgccattgt gtccgtgcgt cccggcgctc gcccagtcag gttgacctaa
     2161 ccccgccgtt ctccggtcgc tcggtgtcgc ccgcgggctc gtcggccagc tcctggggga
     2221 tgacctgcac ctcggtctcc cgctccggat accgcctgcc gacgatgacg aagtaggccg
     2281 ccgcgagcgc gaacaagacg atcgacgtcc acacgttgag ccgtacgccg aagaagtggt
     2341 tggcggtgtc gacccggagg tactcgatcc agactcgacc ggccgtgtag gccatcacgt
     2401 acacggcgaa cgcccggccg tgcccgagct tcagcctgcg gtcgagccag atcacaaggc
     2461 ctgcgacacc gacgtcccac agcgactcgt agagaaaggt cgggtggaac gtcgagtact
     2521 gctggaagtc gccccggtgt gccgggtcga tctccagacc ccacggaagg tcggttggct
     2581 tgccgtacag ctcctggttg aaccagttcc cccaccgtcc cacggcctgc gcgagcgcga
     2641 tgcccggcgc cagcgcgtcc gcgaaggcgg cgaacctcag cttgtggcgt cgacagccga
     2701 gatacgcgcc gagcgccccg agcgagatcg ccccccagat gccgagcccg ccccgccaga
     2761 tgtacagcgc ggtgatcggg ctcttgccct cgccgaagta cagctcggga tcggtgagca
     2821 cgtggtacag ccggccgccc acaagcccga agggcaccgc ccagatcgcc acgtccccca
     2881 cggcgccggg cagtccgcca cgggcgatcc agcgcctttc gccgacccac accgcgacga
     2941 ggacgccgag gatgatgcac agcgcgtacc cgcgcaccgg gaacggtccg atgtgccaga
     3001 caccttcgct cggactgggg atcgacgcca gcgtgatcgc gctcatcggg gcagaaccta
     3061 ccgggcggac tcggccaacg tgtcgatgtc gctgaccatg tcggagatgg gtgtgccgtc
     3121 ggtccagacc accggcgcct cgttgccacg gaaaccgacc acctgggctc cgtgcccgac
     3181 gtcgtagcca ccaccgggca gctgctgccg tccttcgatc gcgacgccca tggcgtcggc
     3241 agctttgacg atgtcaccga ggccaccggt caggccgacg aagtcggggt tgtagtgatc
     3301 gaggtactca cgcagcacgc ccggagtgtc acgtgccggg tcggtcgtga tgaacacgaa
     3361 ctgcgtacgg tgcgccacgt cgcctgggag ctggagcatc gtctgagcca cgtcgctcat
     3421 cacgaggggg cagacatcgg gacagtgcgt gtagccgaag aagacgagcg tcacggggta
     3481 ggcggtgtcc gtgatgaggt tgaacggctg cccgttggtg gcggtgagtg tgatgtcggg
     3541 catccggtac ggcgtcgccg gctcggcgcc gttgaaacgg gggtcgccgc ctgacgacat
     3601 gacgccgccg gagttgtcgg cggagccctg catcaccacc gggtcgccac cgcacgcggc
     3661 caccaccgca cacagcagcg tgaccagcaa cggcagggtg aggtggcgtg cacgccgggc
     3721 tggtggggtc atgccgggcc tgcgtcatga cgtcgacacg gtgcacccac gtggtggttc
     3781 aactccgtaa ccgtggtcaa agttccccgc gtacgccgcg ggacagctca gcggcgagcg
     3841 cctcgactgc gtcgttctcg gcaccttcac cgcgcgcatc ggaccggacc accgcccgga
     3901 cgaatgcgct tccgacgatg accccgtcgg cgtacgacgc gacctccgcg gcctgctcgc
     3961 cgttggagac gccgaggccg accccgatcg gtatgtcggt cacgccacgc gttcggcgga
     4021 cgagctcggg cgtgacgctg ctggtctgtt cgcgcgcacc cgtcacaccc atcaccgcgg
     4081 tcgcgtagac gaaaccgcgg catgcggcgg tcgtcagttc gagtcgtgac gcggtcgacg
     4141 acggcgcaac caggaagacc ttgtcgaggt cgaggtcgtc ggccgccgcc acccattcgg
     4201 cggcttcctc gggaatcagg tcgggagtga tgagccccga cccgcccgcc gcggcaaggt
     4261 cagcggcgaa ccgcttcacc ccgtaacgct cgaccgggtt ccaataggtc atcaccagtg
     4321 tcggaacgag ctccgcgacc gcttccacga ccgcgatgac gtcgccgatc ctggtgcccg
     4381 cccgcagtgc ttggtcagcg gcggcctgga tcgtgggtcc gtccatgacc gggtcggagt
     4441 acggaagccc gacctcgatg acgtcgcagc cgccgcgaac catcgaccgc agcagcgcga
     4501 tcgagcgggc gacgtcgggg aagcccgccg gcagatatcc gacgagggcg gcccggccgt
     4561 ccgccttggc cgcctcgaac gcgtcggccg acccggtcat gcaagcccgc cttccgaggg
     4621 cgccttgtcg acgaggtcga accaggtcgc ggccgtcgac atgtccttgt cgccgcgtcc
     4681 ggacagacag atcgcgagca ccgcgtccgc gcccagctcc gccgcgacgt cgtacgcgcc
     4741 ggcgagcgcg tgggcacttt cgatcgccgg gatgatccct tccgtgcgcg ccagtgccgc
     4801 catcgcgtcc atcgcctgcg cgtcggtgat cggccggtac gtcgcgcgtc cggtctcggc
     4861 cagccaggcg tgctccgggc cgacgccggg atagtcgagc cccgccgaga tcgagtgcga
     4921 ctcgatcgtc tgaccgtcct cgtcctgcaa caggaacgac cgtgtcccgt gcagcacgcc
     4981 gacctctccg gcactgatcg tcgcggcgtg ccggcccgtc gacacaccct ctccgccggc
     5041 ttcgaagccg tacagccgga catcctcgtc gccgaggaag ccggcgaaca tgccgatcgc
     5101 gttggacccg ccgccgacac acgccagtgc tgcgtcgggc agccggccga gcagcgccaa
     5161 cgactgggcc cgcgtctcgt cgccgatgcc gcgcgcgaag tcgcggacca tcgccgggaa
     5221 cggatgcgcc ccggccgcgg tgccgagcag gtagtgcgtg gtctcgacgt tggccaccca
     5281 gtcacgcagc gcctcgttga tggcgtcctt caaggtccgg ctgccggtcg tcaccgggat
     5341 gacctcggcg ccgagcatct tcatacgtgc gacgttgagt gcctgccgct cggtgtcgac
     5401 ctc
//
LOCUS       DATBFZ010000095        14271 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_2351497, whole genome shotgun sequence.
ACCESSION   DATBFZ010000095 DATBFZ010000000
VERSION     DATBFZ010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 14271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14271
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2351497"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(393..1400)
                     /locus_tag="VH419_02840"
     CDS             complement(393..1400)
                     /locus_tag="VH419_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013162151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEY7042583.1"
                     /translation="MKLGAYTACLHDRSLQEALKILSELGLTSAEINAGGFIPAPHLP
                     IEQVLSSASAREDYLGEFEQAGVTLTGLNVNGNPLHPDPEVGPRHAEDLRRAINVAGA
                     LGVKRVVAMSGLPGAEAAAAFPSWVVEPWDSVYLEVLDYQWERVVVPFWRDIDVLARE
                     ADVKVCIEMHPHNVVFNPATLERLIAKTGATHVGAELDPSHLFWQGIDPVAAVEYLGD
                     RVFHAAAKDTRINDANVRIHGVLDDRFHRIPPEENPIGLGGRYTLNRWPEDPAWEFVA
                     VGRGHEVDDFWVPFLRALYAVDPEMAINIEHEDQDFDQIEGLRLAAENLRTAAATAEV
                     V"
     gene            complement(1446..2624)
                     /locus_tag="VH419_02845"
     CDS             complement(1446..2624)
                     /locus_tag="VH419_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY7042584.1"
                     /translation="MAGSSIGVAVIGAGMAGRAHAQGYRSASTVFEPDLPEIRLVAIA
                     DINEALGGDAARRYGYARAEKSWQALLDAPDVDAVSVVVANPLHREIVEALLAAGKHV
                     LCEKPLAPSVADAEAMVEAAAKTDRMSAVGFTFRRSPAINAIRAQIEQGRLGAPMHFS
                     AHYWCDYGSDPKAPMSWRYKGGPGTGALGDIGSHLVDVGEFLCGPVESVQGAVLTTTV
                     PKRALPAGAVVGHAAAELSDTWEPVENEDLASFAVRFANGASGTISVSRVAVGHPNAL
                     AFEVFGTEASASFDMSRAAEFGFADHTSPSVVNGYRQVLVGPEHPYIAGGLAMDFPGV
                     GHGQQDFFTYQSRAFLEQIAGLGRLPACPPLSHGLHNLQLLQAVVSSAEGDGRAVPIT
                     "
     gene            2771..3808
                     /locus_tag="VH419_02850"
     CDS             2771..3808
                     /locus_tag="VH419_02850"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY7042585.1"
                     /translation="MRSTAATGVGVPGRRPRLDDVASAAGVSPASVSLVLNGAPGPSA
                     ATRARVLEVATALGYRADRTASLLARRRRHLLGVMIDIRNSFHAELVEAIQANAEQRG
                     YDVVLSTLTRTRDERRAVETLLDFRCEALILLGPTAPKRQLAALALQLPVIVIGRRIV
                     GTDVDVVRTADQPGLGQVVDHLVALGHERIAYVDGGKGTIATDRRRGYILAMRRHRLS
                     AQQRVVPGGQTEDAGTHAATELLQEQPWPTAVIAFNDRSALGLLDALIREGVDVPGEM
                     SVVGYDDSPPSRLAHVNLSTVSQNPDRQAEHAVVSAVERLDDGRTEPRAIVLDPQFVV
                     RGTTGPARTND"
     gene            3818..4831
                     /locus_tag="VH419_02855"
     CDS             3818..4831
                     /locus_tag="VH419_02855"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY7042586.1"
                     /translation="MSKVRWGLLSTASIGRVVVAACRRARAAEFVAVASRGPERAQRF
                     ADEFGLEASFGSYDELLAADDVDAVYVALPNSMHAEWVQNALEAGKHVLCEKPFAPTA
                     EETITCFDAAKAAGRVCVEGFMYRHHPQTTKFRELLADGAIGELTFVRSALSTRTEPS
                     DIRRSTEMAGGSLADLGAYCASATRLVAGEPLQVFAEQVLDGPQGVDLRFAATIRLEN
                     GVVAQFDTGLDLPRRDELELIGTTGRIVVPDPWLCRSGYVELVRDSETSRIPVDPDGT
                     FALTEADHDVYRIEFDAVSTAILTGSPPVFDATDAVRQARLIDALRASAQDGSPVSLP
                     ALQ"
     gene            complement(5056..6231)
                     /locus_tag="VH419_02860"
     CDS             complement(5056..6231)
                     /locus_tag="VH419_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY7042587.1"
                     /translation="MTPARPALRVAIVGFGWMGQVHARSYARLLHHYPDVPLRPELVV
                     VVDPAPDDRLAVAAATFGFRHQLTDWRALFAEDLVDAVSVTGPNFVHRDVAVAAAQHG
                     KHVWIEKPAGRDVSEAEQIADAVHAANVQSVVGFNYRHVPAVELTRAHVAAGDLGRVE
                     HVTIRLLADYSAHPHGGLSWRFERAMAGTGVLGDLVSHGVDLARFLVGEIAELVSDEA
                     TFIARRPKAAAGGSHYAQVEGGELGEVENEDYVCALLRWANGARGTLEASRVAVGEQC
                     SYGFEIHGDRGALAWDFRRMGELRTCLGQDYLDARWETRFVSRGDGELGVFQPASGIT
                     MGFDDLKVIEARQFVESIAQGKALGPTVDDAVAAARALEAMQTSARERRWVAVTPTR"
     gene            complement(6228..7085)
                     /locus_tag="VH419_02865"
     CDS             complement(6228..7085)
                     /locus_tag="VH419_02865"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEY7042588.1"
                     /translation="MKLALDPYMLRSVPLTELPGVVADLGYEYIELSPREDFLPFFLH
                     PRADRHTIAAFKRALDSAGVQVSSVLPLYRWSGPDEDERQAAVRYWKRAIEVSVELDC
                     KVMNSEFNGRPEQPSLSEAQFWRSMEELLPVFEREGVELRLEPHPDDFVEDGRTAVDM
                     VRGINSDLVSFLYCAPHTFHQGGDITGIMEYAGDLLTHLHVADSFDHRASSGLRYIVN
                     PPGSPARIHQHLDIGQGEVDWDEFFGTLGRLRFDGIMTVCVFAWEERAQESSIFMREE
                     IERRTKDWA"
     gene            complement(7082..8098)
                     /locus_tag="VH419_02870"
     CDS             complement(7082..8098)
                     /locus_tag="VH419_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020390244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY7042589.1"
                     /translation="MTVNIGVIGTGNIGTYHAHRIATELPGAGVVSVFDVDVARAQQV
                     AEAVGARVHEHALDVISDSEADAVLIASPGDLHAEQVLACLDAGKPVLCEKPLATASE
                     DALKVVEAEVGLGRQLIQVGFMRRYDAAYNEVKATLDSGDIGEALVLHCVHRNPTVPP
                     TFREFMAITDSVVHEIDTSRWLLGDEIVAVTVRSGKRSPHAAGQDPQMVHFETASGVL
                     VDVESFVNCQYGYDVRCELVGSEGFAQLENPTFSSVTRAAQRRTGVPADWQERFDNAY
                     QRELLAWVHGVGTGAYSGASAWDGYAATAVAEAGVRSLDEGGRVAVDLVDKPSLYGGK
                     GSTA"
     gene            complement(8201..9013)
                     /locus_tag="VH419_02875"
     CDS             complement(8201..9013)
                     /locus_tag="VH419_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY7042590.1"
                     /translation="MTTEQAVPVEETKTSTPLIQLEDVGKAYGNIVALFDVSLKVGPH
                     EVTCVLGDNGAGKSTLIKIISGREHHTSGVVRVDGEETTLSSPRDALDRGIATVYQDL
                     AVVPLMPVWRNFFLGSEEVKGVWPLRALEVKDMRQKAKDELHKMGIDLPDIDAPIGKL
                     SGGQRQCVAIARAVYFGARVLILDEPTAALGVKQSGVVLRYINRARDHGLGVIFITHN
                     PHHAYMVGDHFILLNRGRMTLDCKYEDITLDQLTQEMAGGDELETLTHELKR"
     gene            complement(9019..10068)
                     /locus_tag="VH419_02880"
     CDS             complement(9019..10068)
                     /locus_tag="VH419_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012919343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY7042591.1"
                     /translation="MTQAPPAPATAPPDERVKAVTPFHRLIARPELGSLVGAIAIYVF
                     FFIVAEPFRTTAALSTILYTSSVIGIMAVGVALLMIGGEFDLSAGVAVTTSALVASLF
                     AWQYSTNVWVGVALALLASLAIGFFNGYLVMRTGIPSFLVTLGSFLMLSGANLAVTKM
                     ITGQVASQNISDMDGFPLAHKIFASQFTIFGVDVWITVVYWFAFVALATWVLLRSKAG
                     NWIYAVGGEAESARAVGVPVSRTKIGLFMTVGFLAWFSGMHILFRFNTVQSGQGVGNE
                     FIYIIAAVIGGCLLTGGYGTAIGSAIGAFIYGMTLQGIVYADWNPDWVKFFLGLMLLG
                     ATVANMWVRKQAERS"
     gene            complement(10136..11137)
                     /locus_tag="VH419_02885"
     CDS             complement(10136..11137)
                     /locus_tag="VH419_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020390247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEY7042592.1"
                     /translation="MTKHRTRWSIVAGAAAMALVLAACSSSGGKQEEDSSADLGAAGK
                     ANTPEVKIAMITHEAPGDTFWDLIRKGAEAAADKDNVDLVYSNDPQAPAQADLIQNAI
                     DQKVDAIATTMPNPDALGPVINKAVDAGIPVVEFNAGVSDLDKTNELMYFGQDESVAG
                     EAAGQRLASEGYKKVLCVVQEQGQVQLEARCAGVQKGFSGTTEKLYVNGRDLPSVTSA
                     IQGKLQTDKDIDAVITLGAPIALAAVQSIQQANSSAKLVTFDTNADLVAAIQDGDVQW
                     AIDQQPFLQGYESIDSLWLYLYNGNILGGGQPVLTGPSFIDQDNIDTVAEYAKRGTR"
     gene            complement(11223..12158)
                     /locus_tag="VH419_02890"
     CDS             complement(11223..12158)
                     /locus_tag="VH419_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020390248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEY7042593.1"
                     /translation="MTTSDNVSQLRLDRMRLGTAPDSWGVWFPDDPNQTPWHRFLDEA
                     AEAGYSWIELGPYGYLPTDPKQLSDELASRGLSLSGAAVFAGLHRGPDAFKQAVADCR
                     QVVDLITALGAHHLILLPEGYTDLDGNITQPVELGPDEWRDLVAGMSELGRLLREESG
                     VRLDFHPHADSHVDTQERVVRLLEDTDPDTVSLCLDTGHISYAGGDNLTIIQRFPERI
                     GYVHLKQVNPAVVDQVQADNLCFAEAVRKGVMIEPPMGEPAMPPVLEALAAVDADLFA
                     IVEQDLYPCSPDVPLPIATRTRRYLEQCGLGARPD"
     gene            complement(12177..13673)
                     /locus_tag="VH419_02895"
     CDS             complement(12177..13673)
                     /locus_tag="VH419_02895"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEY7042594.1"
                     /translation="MRTIEHWIDGKPSSGTSDRRVPVWNPATGEQQADVVLASRADVD
                     TAVASSAAAFTEWSQASLSKRAKIMFAFRELVNANIDRLAEAITDEHGKVLSDARGEV
                     QRGLEVVEFACGIPHLLKGDFSDQVSTGVDTYSFREPLGVVAGITPFNFPIMVPMWMH
                     PVAIACGNTFVLKPSERDPSASMLVAELWQEAGLPDGVFNVVHGDKEAVDALLDNPDV
                     QAVSFVGSTPIARYIHERATGTGKRVQALGGAKNHAIVMPDANLDFASDHLVAAAFGS
                     AGERCMAISAAVAVGEAGDAIVDAVSDKARRVRVGSGRAEDSEMGPVVTAAARDRIVG
                     YIDSGQEQGARLTVDGRGLVVPGYEQGFFVGPTVFDEVTPDMDVYRDEIFGPVLSVVR
                     ASTVDDAIALVNANPYGNGTAIFTSSGEAARTFQRGVHVGMIGINVPIPVPMAFYSFG
                     GWKDSLFGDKHIHGPEGVSFYTRAKVVTSRWPHVDHAIAASYHFPTAH"
     gene            complement(13702..>14271)
                     /locus_tag="VH419_02900"
     CDS             complement(13702..>14271)
                     /locus_tag="VH419_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEY7042595.1"
                     /translation="GYSCMGYEIAGGMGVRMAAPDRDVFVMVGDGSYLMMAQELVTAV
                     QERIKLIVVLVQNHGFASIGELSETLGSQRFGTWYRYRNDDTGRLDGDVLPVDLAANA
                     ESLGVRVLRAHTIGEFRDALLDAKRSDGGPVMVHVETDPLVPAPSSESWWDVPVAEVA
                     QVESTQQARKSYEANKANQRQYLKGADER"
BASE COUNT         2296 a         4855 c         4765 g         2355 t
ORIGIN      
        1 ccccaacctc aacctgtccg attagcggcg taggtggctt ttcggaggcg ggtgggccgc
       61 gccgaggacg ggcaggacgg cagtcctgcc cgacaagcct gcccgaaccg gtgcatcgag
      121 gctcgcgggt caagggacgc cgcaggcggt cgcgtcagcg atcgcgcagc gacccttgac
      181 gcgtgaggaa tgcaccggta gcaggcgcgg gccaccaacg taccggttga gcctgtcgag
      241 acccctcgat ctcgaccagc tcgatcagcg ggcagtccgg cggtcgagcc tgtcgagacc
      301 cctcgatctc gaacagctcg atcagcggga tcacgcctgc ggaatgcgcc ggcagcggga
      361 gtgcctcgca ccaagcggaa tctgcctggg ggtcagacga cttcggcggt ggcggcggcg
      421 gtcctcaggt tctcggccgc gagccgcagc ccctcgatct ggtcgaaatc ctggtcctcg
      481 tgctcgatgt tgatcgccat ctccgggtcg accgcgtaca aggcccggag gaacgggacc
      541 cagaagtcgt ccacctcgtg gcctcggccg acggcgacga actcccacgc cgggtcctcc
      601 ggccagcggt tcaaggtgta gcgaccgccg agcccgatcg ggttctcctc gggcgggatc
      661 cggtggaacc ggtcgtcgag gacaccgtgg atgcggacgt tcgcgtcgtt gatccgggtg
      721 tccttcgcgg cggcgtggaa gacccgatcg cccagatact cgaccgctgc gaccgggtcg
      781 atgccctgcc agaacaggtg gctcgggtcg agctcggcac cgacgtgcgt ggcgccggtc
      841 ttggcgatca gccgctccaa cgtcgcgggg ttgaacacga cgttgtgcgg gtgcatctcg
      901 atacagacct tgacgtccgc ctcgcgagcc agcacatcga tgtctcgcca gaacggtacg
      961 acgactcgct cccactggta gtcgaggact tccaggtaca cgctgtccca cggctcgacg
     1021 acccacgacg ggaatgcggc ggccgcctcg gcgccgggaa ggcccgacat cgccaccacc
     1081 cgcttgaccc cgagtgcccc cgcaacgttg atcgcgcgac gcaggtcttc ggcgtgtcgc
     1141 ggtccgacct cggggtcagg gtgcagcggg ttgccgttga cgttcagccc ggtgagcgtg
     1201 acaccggcct gctcgaactc tccgagatag tcctcgcgcg ccgaagcact ggacagcacc
     1261 tgttcgatcg gcagatgcgg tgcagggatg aatccgccgg cgttgatctc ggcgctggtg
     1321 aggccgagct cggacaggat cttcaacgct tcctgcagcg accggtcatg caggcacgcg
     1381 gtgtaggcgc caagcttcat gaggtcccct tcttgctggt tcgtcggtac gccggactgg
     1441 tcgtctcagg tgatcggcac agcccttccg tcgccctcgg cagaggacac gacggcctgg
     1501 agcagctgca ggttgtggag tccgtgggag agcggcgggc acgcaggcag gcgacccagg
     1561 ccggcgatct gctcgagaaa cgcgcgcgac tggtaggtga agaagtcctg ctggccgtgc
     1621 ccgacccccg ggaagtccat ggccaggccg cccgcgatgt agggatgctc gggaccgaca
     1681 agcacctggc ggtagccgtt caccacgctc ggcgaggtgt ggtcggcgaa tccgaactcg
     1741 gccgcacgcg acatgtcgaa cgacgccgag gcctcggtgc cgaagacctc gaacgccagg
     1801 gcgttcggat ggccgaccgc cacccgcgac acggagatcg tgccgctcgc gccgttggca
     1861 aagcgaacgg cgaagctcgc cagatcctcg ttctcgaccg gctcccaggt gtcgctgagc
     1921 tcagccgcgg cgtgcccgac caccgcgcct gccggcagcg cccgcttcgg gacggtcgtc
     1981 gtcaggaccg cgccctggac gctctcgacc ggaccgcaga ggaactcacc gacgtcgacg
     2041 aggtggctgc cgatgtcacc cagggcaccg gtcccgggac cgcccttgta tcgccagctc
     2101 atcggcgcct tcggatccga gccgtagtcg caccagtagt gcgcgctgaa gtgcatggga
     2161 gcacccagcc gcccctgctc gatctgcgcg cggatcgcgt tgatcgccgg cgaccggcgg
     2221 aacgtgaagc ccacggccga catccggtcg gtcttggcag ccgcctcgac catcgcctcg
     2281 gcgtcagcca ccgatggagc cagcggcttc tcgcagagca cgtgcttccc ggccgccagc
     2341 agcgcctcca cgatctcccg gtgcagtggg ttggccacca cgacgctgac ggcgtccacg
     2401 tccggcgcat cgagcagtgc ctgccaggac ttctccgcgc gggcgtagcc gtatcgccgg
     2461 gcggcgtctc cacccaatgc ctcgttgatg tccgcgatcg cgacgagccg aatctccggc
     2521 aggtccggtt cgaacaccgt cgatgccgac cggtagccct gggcgtgtgc gcgacccgcc
     2581 atcccggctc cgatcaccgc gaccccgatc gaggatcctg ccatggtcgc tccctttccg
     2641 acgagtccac tgcagctgct tcggccagct ggacccaaca tcttttaaag cgcttcaagt
     2701 ccttgctact atcggccatg ccgcatcgat gtgtcaaggg ttgcagtcgc tcactcggag
     2761 ggatcgcgtg gtgagatcca ctgccgcaac cggagtaggc gtccccggtc gccgaccccg
     2821 gctcgacgac gtggccagcg cggccggggt ctcgcccgcg tcggtctccc tcgtcctgaa
     2881 cggcgcgccc gggccgagtg ccgccacgcg agcgcgggtc ctcgaggtgg cgacggcgct
     2941 cggctaccgc gccgaccgga ctgcgagtct gctcgcccgc cggcggcgtc acctgctcgg
     3001 cgtcatgatc gacatccgca actcgttcca cgcagagctc gtcgaagcga tccaggccaa
     3061 cgcagagcag cgcggctacg acgtcgtgct cagcacgctg acccggacga gagatgaacg
     3121 ccgtgctgtc gagaccttgc tcgacttccg ttgtgaggcg ctgatcctgc tcggcccgac
     3181 agcgcccaag cggcagctgg ccgcgctcgc actccagctg cccgtgatcg tgatcgggcg
     3241 ccgaatcgtc ggtaccgacg tcgacgtcgt acgcacggct gaccagccag ggctgggtca
     3301 ggtggtcgat cacctcgtgg cgctcggcca cgagcgtatt gcgtacgtcg acggcggcaa
     3361 gggcacgatc gccaccgacc gccgtcgcgg ctacatcctg gcgatgcgcc ggcaccggct
     3421 gagcgctcag cagcgggtgg ttcccggtgg gcagaccgag gacgccggta cacacgcagc
     3481 cacggagctg ctccaggagc agccgtggcc cacggctgtc atcgcgttca acgaccgcag
     3541 tgccctcggc ctgctcgacg cgctcatccg tgagggagtc gatgttcccg gcgagatgtc
     3601 ggtcgtgggg tacgacgaca gcccgccctc gcgactcgcc cacgtcaacc tgtcaacggt
     3661 cagccagaac cctgaccgcc aggcggagca cgcggtcgtg tcggccgtcg agcggctcga
     3721 tgacggtcgc accgagccac gcgccatcgt cctcgacccg cagttcgtcg tccgcggcac
     3781 caccggcccg gctcgcacca acgactagga ggcgatcatg tccaaggtcc gctggggact
     3841 cctctccacg gcttccatcg gccgggtcgt cgtggccgcc tgccggcggg cgcgggccgc
     3901 agagttcgtc gcggtcgcca gccggggacc cgagcgcgcg cagcgcttcg cagacgagtt
     3961 cggcctcgaa gcgagcttcg gctcgtacga cgagttgctc gccgccgatg atgtcgacgc
     4021 cgtctacgtc gccctcccga actcgatgca cgccgagtgg gtgcagaacg cgttggaagc
     4081 aggcaagcac gtgctctgcg agaagccctt cgcacccacg gccgaggaga ccatcacgtg
     4141 tttcgatgcc gcgaaggcgg ccgggcgcgt gtgcgtcgag gggttcatgt atcgccatca
     4201 cccccagacg acgaagttcc gcgagctgct cgcggacgga gccatcggag agctcacgtt
     4261 cgtgcggtcc gcgttgagca cgcgcaccga gcccagcgac atccgccgct cgaccgagat
     4321 ggcgggcggc tccttggctg acctcggcgc ctactgcgca agtgccaccc gcctcgtcgc
     4381 cggcgagccg ctgcaggtgt tcgccgagca ggtcctggac ggcccgcaag gtgtcgacct
     4441 gcgcttcgcg gcgacgatcc ggttggagaa cggcgtcgtt gcacagttcg acaccggctt
     4501 ggacctcccg cggcgcgacg agctcgagct gatcgggacg accgggcgga tcgtcgtacc
     4561 agatccctgg ctgtgtcgat cggggtacgt cgaactggtc cgcgacagcg agaccagccg
     4621 gatccccgtg gacccggacg gtacgttcgc gctgaccgag gcagaccacg acgtctaccg
     4681 catcgagttc gacgccgtct ccaccgccat cctcaccggg tcgccgccgg tcttcgacgc
     4741 caccgacgcg gtccggcaag cgcgattgat cgatgcgctg cgcgcctccg cgcaggacgg
     4801 gagcccggtg tccctgccgg cgcttcagtg agaccgtcgg cattcctgcc tcaactggcc
     4861 cagggggtac ccgctgaact ggccaaggat gacgcagcct ggttcgccgc acgtacacgg
     4921 tcgttcggac atccgccgcc cgacgaccgc ggtgtgcgat ggttccatcg cagacccatg
     4981 ccttgcatcc gaggtttgcg atgttgacag cccgcctgca gtctttccat cgcaaaccga
     5041 ggccggcccg ccgcgtcagc gggttggtgt gacggcaacc catcggcgtt cgcgtgccga
     5101 cgtctgcatc gcctccagtg ctcgggcggc tgccacggcg tcgtcaacgg tcgggccgag
     5161 tgctttgccc tgggcgatcg actcgacgaa ctggcgcgcc tcgatcacct tcaggtcgtc
     5221 gaaccccatc gtgatcccgc tggccggctg gaacaccccc agctcgccat ccccgcgaga
     5281 aacgaacctg gtctcccagc gcgcgtccag gtagtcctga ccgaggcagg tccgcagctc
     5341 acccatccgg cggaagtccc acgccagcgc accgcgatcg ccgtggatct cgaagccgta
     5401 ggagcactgt tcgccgacgg cgacgcggct cgcctcgagc gtgccacggg cgccgttcgc
     5461 ccagcgcagg agcgcgcaca cgtagtcctc gttctcgacc tcgccgagct caccgccctc
     5521 gacctgcgcg tagtgcgagc caccggccgc cgccttcggg cgacgggcga tgaacgtggc
     5581 ctcgtcgctg acgagttccg cgatctcccc caccaggaac ctggccaggt ccaccccgtg
     5641 actgaccagg tcaccgagca ctccggtacc ggccatcgcg cgctcgaagc gccaggacag
     5701 tccgccgtgc gggtgggccg agtagtcggc cagcagccgg atcgtgacgt gctcgacgcg
     5761 accgagatcg ccggccgcga cgtgagcccg ggtcagctcg acggccggga cgtggcggta
     5821 gttgaagccg acgacggact ggacgttcgc ggcgtgcacc gcgtcggcga tctgctccgc
     5881 ctcggacacg tcccgtccgg caggtttctc gatccagacg tgcttgccgt gttgggcggc
     5941 cgcgaccgcc acgtcgcggt gcacgaagtt cgggccggtg acactgacgg cgtcgacgag
     6001 gtcctcggcg aacagggccc gccagtcggt cagctgatga cggaagccga acgtcgccgc
     6061 agcaaccgcg agccggtcgt ccggggctgg atcgacgacg accaccagct cgggacgcag
     6121 cggcacgtcc ggatagtgat gcagcagtcg ggcgtagctt cgggcgtgca cctggcccat
     6181 ccacccgaag ccgacgatcg caacgcgcag cgcaggtcgc gcaggggtca cgcccagtcc
     6241 ttcgtacgcc gctcgatctc ctcacgcatg aagatgctgg actcctgggc gcgctcctcc
     6301 cacgcgaaca cgcagacggt catgatgccg tcgaaccgca accggccaag tgtgccgaag
     6361 aactcgtccc agtcgacctc gccctgcccg atgtcgaggt gctggtggat ccgagcaggc
     6421 gagcctggcg ggttgacgat gtagcgcagt ccactcgacg cgcggtggtc gaacgagtcg
     6481 gccacgtgca ggtgcgtgag gaggtcgccg gcgtactcca tgatcccggt gatgtcgccg
     6541 ccctggtgga aggtgtgtgg agcgcagtac aggaacgaca ccaggtcgga gttgatgccg
     6601 cgcaccatgt cgacggccgt ccggccgtcc tcgacgaagt cgtccggatg cggctccagc
     6661 cggagctcga ccccctcccg ttcgaacacc ggcagcagct cttccatgga gcgccagaac
     6721 tgcgcctcgc tgaggctggg ttgctcgggc cgaccgttga actcggagtt catgaccttg
     6781 cagtcgagct ccaccgacac ctcgatcgcg cgcttccagt agcggaccgc ggcctgacgc
     6841 tcgtcctcgt cggggcctga ccagcggtac agcggaagca ccgacgagac ctgcacccct
     6901 gcactgtcga gcgcccgctt gaacgcggcg atcgtgtgac ggtcggccct cgggtgcagg
     6961 aagaacggca ggaagtcctc acgaggagac agctcgatgt actcgtagcc gagatcggcg
     7021 accacaccag gcagctcggt cagcgggacc gagcggagca tgtacggatc gagggcgagc
     7081 ttcatgccgt ggaccccttt ccgccgtaca ggctcggctt gtcgacgagg tcgaccgcga
     7141 cgcggccgcc ttcgtcgagc gaccgcacgc ccgcctcggc caccgcagtc gcagcgtagc
     7201 cgtcccatgc gctggcaccg gagtacgcac cggtcccgac tccgtggacc caggcgagga
     7261 gctcgcgttg gtaggcgttg tcgaaacgct cctgccagtc cgctggtacg ccggtacgcc
     7321 gctgggctgc ccgcgtcacc gacgagaacg tcgggttctc cagctgtgcg aacccttccg
     7381 accccaccag ctcacatcgc acgtcgtagc cgtactgaca gttgacgaac gactccacgt
     7441 ccacgagcac gccgctggcg gtctcgaagt ggaccatctg cgggtcctgt cccgccgcgt
     7501 gcggcgaccg tttgccggat cgcacggtca ccgcgacgat ctcgtcgccg agcaaccacc
     7561 ggctggtgtc gatctcgtgg acgaccgagt cggtgatggc catgaactcg cggaatgtcg
     7621 gcgggaccgt ggggttgcgg tggacacagt gcaggacgag tgcctcaccg atgtcgccgc
     7681 tgtccaaggt cgccttgacc tcgttgtacg ccgcgtcgta ccgacgcatg aagccgacct
     7741 ggatcagctg tcggcccagc ccgacctcgg cctccaccac tttcagagca tcctccgacg
     7801 cggtggcgag cggcttctca cagagcaccg gtttgcccgc gtccagacag gcaaggacct
     7861 gttcagcatg cagatcccca ggtgaggcaa tgagcacggc gtccgcctcc gagtccgaga
     7921 tcacgtcgag cgcgtgctcg tggacgcggg ctccgaccgc ttcggcaacc tgctgggcgc
     7981 gtgcgacgtc cacgtcgaag acggagacga cgccggcgcc cggcagctcg gtggcgatgc
     8041 ggtgcgcgtg atacgtgccg atgttgccgg tgccgatcac gccgatgttg acggtcatgg
     8101 gcgatgtcct tgctcgttgg gcaagttcct ggccgacccg gtggtcgcgt cgaccaggaa
     8161 ctcgacaggg ctcgacaggc tcgacagaag gtgcggcggg tcagcgtttg agctcgtggg
     8221 tcagtgtctc gagctcgtcg ccgccggcca tctcctgggt cagctggtcc agcgtgatgt
     8281 cctcgtactt gcagtccaac gtcatcctgc cgcggttcag caggatgaag tggtccccga
     8341 ccatgtaggc atggtgtggg ttgtgggtga tgaagatgac gccgagcccg tggtcgcggg
     8401 ctctgttgat gtagcgcagc accacacctg actgcttgac cccgagcgcc gccgtcggct
     8461 cgtcgaggat cagcacccgg gcaccgaagt agaccgcgcg ggcgatggcg acgcactggc
     8521 gttgacctcc ggacagcttg ccgatcgggg cgtcgatgtc cgggaggtcg atgcccatct
     8581 tgtggagctc gtccttcgcc ttctggcgca tgtccttgac ctcgagcgca cgcaacggcc
     8641 agacaccctt gacctcttcg gagccgagga agaagttgcg ccacaccggc atcagcggta
     8701 cgacggccag gtcctggtac accgtggcga tgccacggtc caaggcgtcc cgcggtgacg
     8761 acaacgtcgt ctcctcgccg tcgactcgca ccacgccgga ggtgtgatgc tcgcgacccg
     8821 agatgatctt gatcagcgtg gacttgccgg cgccgttgtc gccgagcaca caggtgacct
     8881 cgtgcggacc gaccttcagg ctgacatcga acaacgcgac gatgttgccg taggccttgc
     8941 cgacgtcttc gagctggatc aacggtgtcg aggtcttggt ctcctcgacg ggaacggctt
     9001 gctctgtcgt catgtcgctc aactcctctc tgcctgcttg cgaacccaca tgttcgcgac
     9061 ggtcgcgccg agcagcatga ggccgaggaa gaacttcacc cagtccgggt tccagtcggc
     9121 gtacacgatg ccctggaggg tcatgccgta gatgaacgct ccgatggcgg atccgatcgc
     9181 cgtgccgtag ccgccggtca gcagacaccc gccgatgacg gcggcgatga tgtagatgaa
     9241 ctcgttcccg acaccttggc cggactgcac cgtgttgaac cggaacagga tgtgcatgcc
     9301 ggagaaccag gccaggaagc cgaccgtcat gaacaggccg atcttcgttc gcgacacggg
     9361 cacaccgacg gctcgtgcgc tctcggcttc gccgccgacc gcgtagatcc agttgccggc
     9421 cttcgagcgg agcagcaccc acgtcgcaag tgcgacgaag gcgaaccagt acacgaccgt
     9481 gatccagacg tcgacgccga agatggtgaa ctgcgacgcg aagatcttgt gcgcaagcgg
     9541 gaacccgtcc atgtcagaga tgttctggct cgccacctgg ccggtgatca tcttggtgac
     9601 ggccaggttc gcgcccgaga gcatcaggaa cgagccgagc gtcaccagga agctcggtat
     9661 gccggtgcgc atgacgagat agccgttgaa gaagccgatc gctagcgaag caagcagcgc
     9721 gagggccacg cccacccaga cgttggtgct gtactgccaa gcgaacaagg aagcaacgag
     9781 ggctgacgtc gtgacagcga caccggccga caggtcgaac tcgccaccga tcatcaacag
     9841 cgccacaccc acggccatga tgccgatcac cgacgaggtg tagaggatcg tggagagtgc
     9901 tgccgtggtg cggaacggtt cggcgacgat gaagaagaag acgtagatcg cgattgcgcc
     9961 gaccagcgat ccgagttctg gacgggcgat cagccggtgg aatggggtga ctgccttgac
    10021 ccgttcgtcc gggggggccg tcgctggtgc tgggggtgcc tgagtcatga gagctctcca
    10081 atgtgctgag gaggtgcgtg cccgggggcg gtggtgacac cgcccccggg aacggtcacc
    10141 gggttccgcg cttcgcgtac tccgcgacgg tgtcgatgtt gtcctggtcg atgaacgacg
    10201 ggccggtcag gaccggctga cctccgccga ggatgttgcc gttgtagagg tacagccaca
    10261 gcgagtcgat ggactcgtag ccctggagga acggctgctg gtcgatcgcc cactggacgt
    10321 caccgtcctg gatcgcggcg acgaggtcgg cgttggtgtc gaacgtgacg agcttggccg
    10381 agctgttggc ctgctggatc gactgaaccg cagccagtgc gatcggcgcg cccagcgtga
    10441 tgaccgcgtc gatgtccttg tctgtctgga gcttgccctg gatggctgag gtcaccgacg
    10501 gcaggtcgcg cccgttgacg tagagcttct ccgtggtccc ggagaacccc ttctgcacac
    10561 ctgcgcaccg cgcctcgagc tgcacctggc cctgctcctg cacgacgcag aggaccttct
    10621 tgtatccctc cgaggccagc cgctgcccgg ccgcctcacc tgccacggac tcgtcctggc
    10681 cgaagtacat cagctcgttg gtcttgtcca ggtcgctcac accggcgttg aactcgacga
    10741 caggaattcc ggcgtcgacg gccttgttga tcaccggacc cagcgcgtca gggttcggca
    10801 tggtcgtcgc gatcgcgtcg accttctggt cgatggcgtt ctggatcagg tcggcctgag
    10861 ccggcgcctg cgggtcgttc gagtagacca gatccacgtt gtccttgtcc gccgcggcct
    10921 cggcgccctt gcggatgagg tcccagaacg tgtcgccggg agcctcgtga gtgatcatcg
    10981 cgatcttgac ctcaggcgtg ttggccttac cagctgcacc caggtccgcc gagctgtcct
    11041 cttcttgctt gccacctgag ctgctgcatg ccgcaaggac gagcgccatg gcggcagccc
    11101 ctgcaacgat gctccagcga gtgcgatgtt tggtcatgtg cgtgtgcctt cctaggcgat
    11161 ctttcgggtg agggtgggtg accgggcgcg ataccgaacg ctggtcgtag gtgctgttgc
    11221 ggtcagtcgg gtcgggcacc gaggccgcac tgttcgagat agcgacgcgt gcgagtcgcg
    11281 atcggcaggg ggacgtcggg actgcacggg tagaggtctt gctcgacgat ggcgaacagg
    11341 tccgcgtcga cggctgcgag cgcttcgagg accggcggca tcgccggttc acccatcggc
    11401 ggctcgatca tcactccctt gcggacggct tcggcgaagc agaggttgtc tgcctgcacc
    11461 tggtcgacca ccgcggggtt gacctgcttg aggtggacgt agccgatgcg ctcgggaaac
    11521 cgttggatga tcgtgaggtt gtcgccgccg gcgtacgaga tgtggccggt gtcgagacac
    11581 aacgagaccg tgtccgggtc ggtgtcctcg agaagcctga cgacacgctc ctgtgtgtcg
    11641 acatggctgt cggcgtgggg gtggaagtcg aggcggacgc cggattcctc gcgaagcaac
    11701 cgcccgagct ccgacattcc ggcgacgagg tcgcgccact cgtcagggcc cagctcgacg
    11761 ggttgcgtga tgtttccgtc caggtccgtg tagccctccg gcaacaggat cagatggtgc
    11821 gcgcccagag cagtgatcag gtccaccacc tggcggcagt ccgcgacggc ctgcttgaac
    11881 gcgtccgggc cacgatgaag tccggcgaac acggcggcgc cggacagcga gagtccgcgg
    11941 ctcgccagct cgtcgctgag ctgcttcgga tctgtcggga ggtagccgta cggaccgagt
    12001 tcgatccacg agtatccggc ctcggccgcc tcgtcgagga agcgatgcca cggcgtctgg
    12061 ttcgggtcgt cggggaacca gacgccccac gagtccgggg cggtcccgag acgcatccgg
    12121 tccagcctga gctgagacac gttgtcggag gtggtcatga cgggtcctcg gtgtcctcag
    12181 tgcgcggtgg ggaagtggta gctggcggcg atggcatggt cgacatgggg ccatcggctg
    12241 gtcacgacct tggcccgggt gtagaacgag accccctcag gtccgtggat gtgcttgtcg
    12301 ccgaagagcg agtccttcca acctccgaag gagtagaagg ccatcggcac cgggatgggc
    12361 acgttgatcc cgatcatgcc gacgtgcacg ccgcgctgga atgtgcgcgc agcctcacca
    12421 ctgctcgtga agatcgccgt cccgttgccg taggggttgg cgttcaccag cgcaatggcg
    12481 tcgtccacgg tcgaagccct gacgaccgag agaaccggac cgaagatctc gtcccggtag
    12541 acgtccatgt ccggcgtcac ctcgtcgaac acggtcgggc cgacgaagaa gccttgctcg
    12601 tagccgggca cgaccagccc gcgtccgtcg acggtcagcc gagccccctg ttcctggccg
    12661 gagtcgatgt acccgacgat gcggtcgcgc gccgccgctg tcaccaccgg ccccatctcg
    12721 ctgtcttcgg cgcggccgga gccgacccgt acccgccgag ccttgtcgct gaccgcgtcg
    12781 acgatggcgt caccggcctc cccgacggcc accgccgcag agatggccat gcagcgctcc
    12841 cccgccgagc cgaacgcggc cgccacgaga tggtcggagg cgaagtcgag gttcgcgtcc
    12901 ggcatcacga tggcgtggtt cttcgctcca cccagcgcct ggacgcgctt gccggtcccg
    12961 gtggcgcgct cgtggatgta ccgcgcgatc ggggtcgacc cgacgaacga gacggcctgc
    13021 acgtccgggt tgtcgagcag tgcgtccacg gcctccttgt cgccatggac gacgttgaag
    13081 actccgtcgg gaagtccggc ctcctgccac agctcggcga ccagcatcga cgccgacgga
    13141 tcgcgctcac tgggcttcaa cacgaaggtg ttcccacagg cgatcgcgac cggatgcatc
    13201 cacatcggca ccatgatcgg aaagttgaac ggggtgatcc cggcgacgac accgagcggc
    13261 tcacggaacg agtaggtgtc cacaccggtc gagacctggt cggagaagtc gcccttgagc
    13321 aggtgcggga tcccgcaggc gaactcgacc acctcgaggc cacgctgcac ctcaccgcgg
    13381 gcatcggaca gcacctttcc gtgctcgtcg gtgatcgcct cggccagccg gtcgatgttg
    13441 gcgttcacca gctcgcggaa cgcgaacatg atcttggccc gcttgctcaa cgaagcctgc
    13501 gaccactcgg tgaacgcagc agctgaactg gcgactgcgg tgtcgacgtc ggctctggag
    13561 gccaggacga cgtcggcctg ctgttcaccg gtcgccgggt tccacaccgg tacgcgtcgg
    13621 tccgaggtgc cggaggacgg cttcccgtcg atccagtgtt cgatggtcct cactgatctt
    13681 gcctttcgtg gatcatggtc gtcatctctc gtccgcgcct ttcaggtact ggcgctggtt
    13741 cgccttgttg gcttcatagg acttcctggc ctgctgggtc gactcgacct gcgccacctc
    13801 agcgacgggg acgtcccacc acgactcgct gctcggggcc ggcaccagcg gatcggtctc
    13861 gacgtggacc atgaccgggc cgccatcact tcgcttcgcg tcaagcagcg cgtcgcggaa
    13921 ctcaccgatg gtgtgggcac gcagcacccg aacgccgagg ctctccgcgt tggcggcgag
    13981 gtcgaccggc aggacgtcgc cgtcgagccg cccggtgtcg tcgttgcggt accggtacca
    14041 ggtcccgaag cgctgggacc cgagcgtctc cgaaagctcg ccgatcgacg cgaaaccgtg
    14101 gttctgcacg aggacgacga tgagcttgat gcgctcctgc accgcggtca ccagctcctg
    14161 cgccatcatc aggtacgacc cgtcgcccac catgacgaac acgtcgcggt ccggcgcggc
    14221 catccgtacg cccatcccgc ccgcgatctc gtagcccatg caggagtacc c
//
LOCUS       DATBFZ010000096         3914 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_2024779, whole genome shotgun sequence.
ACCESSION   DATBFZ010000096 DATBFZ010000000
VERSION     DATBFZ010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 3914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3914
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2024779"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1476)
                     /locus_tag="VH419_02905"
     CDS             complement(<1..1476)
                     /locus_tag="VH419_02905"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HEY7042596.1"
                     /translation="MSVLLSSPASAARPRPKTPVPTAYAHQVLNRMSFGFCERTFAQL
                     ERDGGGRAWFHRQLHPASIKDPRGHEVDAWWPDRMVSANQKWKNVFADRKSAWEYAVD
                     LANWTLMRRTQSSRQLLEVMTDFWSNHFHIPATHDLAWCYRNHYDATIRTRALGRFDD
                     LLVAVSLHPSMRLFLNNFESTRDVPNENQGRELLELHSVGREAGYTEQMVKDSARILS
                     GYTVAVWDDWHAYYDPARHATGQVRVLGFRHGNGHSDGRAVTEKYLRYLAHHPSTARN
                     IATKLALRFVSDKPSAQLVNYLAQRFRASGTDVSATLQALIEHPDFAASRGGKVRNPI
                     EDFLATVRVLGVTAHKPTRSESQFARSQIWMCQGQYPFQWPRPDGMPQHSAAWASASS
                     VLNSFDTHYSLSGGWIGEKGSVTYRTRRSWLPEKALRFDHFVDHLCRVLLGRPSNDRL
                     QHAARQATGCRSAEIVDADHQLVSWMMPRLLTAILDTPEHMS"
     gene            complement(1569..2102)
                     /locus_tag="VH419_02910"
     CDS             complement(1569..2102)
                     /locus_tag="VH419_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY7042597.1"
                     /translation="MTGKVKRPPVRRQRAADSIRQDVATYVAAGADESVQRVITSVHG
                     LSRRLNQWYDRQLADLDVSTGEWAVLSKLARDGGEKGLTPSVLADAAGVAPSSMTHRL
                     DRMAERGLVSRLTDPDNRVRVLVSLTDEGWALFRSAIQESDMVEADILGPLSQRERVR
                     LAELLDVVIDGLDGLET"
     gene            2234..3556
                     /locus_tag="VH419_02915"
     CDS             2234..3556
                     /locus_tag="VH419_02915"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY7042598.1"
                     /translation="MGRTRLITAAAFTGSLGWGAILPFQYAYVVDARHWGAAAGMLTG
                     TLFCVGAVVAAPLAGRLADRYSAGRLAVVVSLIAAMATLAMGLAGSVTTFLAGMLVLG
                     AAVTAAAPATQVLVLESVPAADRRSVFAYQFTSQALGMAVGAFAAGLVVEVDTPTGMW
                     PAFAAATLGFVVTAAFLAAATRTAADLTAATRWGQEVSTGSTAGSGSTAELDGDGWRT
                     YRFLARNRQVRLLGLLSIALAAGFYAQFETGLPAFALQSLSVEPTVVGTAAAVNCVVI
                     VGLQWLVVRLTGSQSGAALLAVVGGIWVASWLLLEVALFVSPQVAGVMFVVTFAIFAV
                     GETMYAPILSPLTAAVAPAGLVGTTLGALAALRTGISAVGPLVAGVLFAFDLPHVFVL
                     GHVAINAAAIALAVRLHQAQTAPTSDVDTVAQGAPDRSGGTDAEPVPA"
     gene            3595..>3914
                     /locus_tag="VH419_02920"
     CDS             3595..>3914
                     /locus_tag="VH419_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017934758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HEY7042599.1"
                     /translation="MSDRFQPATYPVPASDALVEFMRTGWSDEDDVVARLDVASWAEQ
                     RRAKLVERFPGERIIVPAGPLKPRANDTDYRFRAETAHVYLTGNQTSDAVLVIDDGEA
                     TLFFR"
BASE COUNT          556 a         1343 c         1354 g          661 t
ORIGIN      
        1 ggacatgtgc tccggggtgt cgaggatcgc cgtcaacagg cgcggcatca tccagctcac
       61 gagctggtgg tccgcgtcga cgatctcggc ggagcggcaa ccggtcgcct gccgagccgc
      121 gtgctgcagg cgatcgttcg agggtcggcc gagcaagacc cggcagagat ggtcgacgaa
      181 gtggtcgaag cggagcgcct tctccggcaa ccaggaccgg cgggtccggt acgtgaccga
      241 gcccttctct ccgatccaac ctccggacag cgagtagtgc gtgtcgaagg agttcagcac
      301 gctgctcgcc gatgcccagg ccgcactgtg ctgcggcatt ccgtccgggc gcggccactg
      361 gaaggggtac tggccctggc acatccagat ctgcgaccgg gcgaactggc tctccgaccg
      421 ggtcggcttg tgcgcagtga cgcccaggac tcgcacggtg gccagaaagt cctcgatcgg
      481 gttgcgtacc ttgccgcccc gcgatgccgc gaagtccggg tgctcgatca gggcctgcag
      541 ggtcgccgac acgtcggtgc cactggcccg gaaccgctga gcgaggtagt tgaccagctg
      601 tgccgacggc ttgtccgaca cgaagcgcag cgccagcttg gtcgcgatgt tgcgggcggt
      661 cgacgggtgg tgggcgaggt agcgcaggta cttctcggtg acggctctcc cgtcggagtg
      721 gccgtttccg tggcggaacc cgagcacgcg cacctggccc gtcgcatgcc gggccgggtc
      781 gtagtacgcg tgccagtcgt cccagaccgc gacggtgtac cccgacagga tcctcgccga
      841 gtccttcacc atctgttcgg tgtagcccgc ctcgcgcccc acgctgtgca gctcgagcag
      901 ctcccgtccc tggttctcgt tcgggacgtc tcgggtcgac tcgaagttgt tgaggaagag
      961 tcgcatggag gggtgcaggc tgaccgcgac caacaggtcg tcgaaccgtc cgagggcgcg
     1021 ggtgcggatc gtcgcgtcgt agtggttgcg gtagcaccag gcgaggtcat gggtcgccgg
     1081 gatgtggaag tggttcgacc agaagtcggt catcacttcg agcagctggc gcgacgactg
     1141 cgtacgccgc atcagggtcc agttggccag gtcgacggcg tactcccagg ccgacttcct
     1201 gtccgcgaag acgttcttcc acttctggtt cgccgagacc atccggtccg gccaccaggc
     1261 gtcgacctcg tgccctcgag ggtccttgat cgacgcggga tggagctggc gatggaacca
     1321 ggcccggcca cccccgtcgc gctccagctg cgcgaacgtg cgctcgcaga agccgaacga
     1381 catccggttc agcacctggt gcgcgtacgc cgtcgggacc ggcgtcttgg gccgcggccg
     1441 cgcagcggaa gcgggcgagc tgaggagaac cgacacagtc acccatggtg atcgaccgca
     1501 tacaacgagt cagcgatgtc cccaatgtcc agccaaagat catccggatg gccgaccctc
     1561 gggcgcggtt acgtctcgag accgtccagc ccgtcgatca cgacgtcgag caactccgcc
     1621 agccggaccc gctcccgctg cgacagcggg ccgaggatgt cggcctccac catgtcggac
     1681 tcctggatcg ccgagcggaa cagcgcccat ccctcgtcgg tcagcgagac cagcacgcgc
     1741 acccggttgt cggggtcggt cagccgcgac accagcccgc gttcggccat ccggtcgagc
     1801 cgatgggtca tcgacgacgg cgcgacgcca gcagcgtcgg ccaggacgct gggcgtcagc
     1861 cccttctcgc ccccgtcgcg agccagcttg ctgagcaccg cccactcccc cgtcgacacg
     1921 tcgagatcgg cgagctgccg gtcgtaccac tggttgagtc gccgggacag tccgtgcacg
     1981 gacgtgatca cgcgctgcac ggactcgtcg gcgccggccg cgacataggt cgccacatcc
     2041 tgacggatgg agtcggctgc ccgttgccgg cgtacgggcg ggcgcttcac cttgcccgtc
     2101 atacccgaag tgtgccacat cacagatttt gatttcgaag tttgcaatct cgaacgtaga
     2161 cttgttcgtg aagcaagagt tcgaaatcga agccgacagg agtcggccgc gccagaaagt
     2221 cgaaggtcac gtcgtgggtc gcacccggct catcaccgct gccgcgttca ccggaagtct
     2281 cggttggggc gccatcctcc cgttccagta cgcgtacgtc gtcgacgcac gccactgggg
     2341 cgccgctgcc ggcatgctca cggggacgct gttctgcgtc ggtgccgtcg tcgctgcgcc
     2401 tctggcggga cgtctcgccg accggtactc cgcaggccgg cttgccgtcg tggtctcgct
     2461 gatcgcggcc atggcgaccc ttgccatggg cctggccggg tcggtgacga ccttcctcgc
     2521 cgggatgctg gtcttaggtg cggcggtgac ggcagcggct ccggcgacgc aggtgctggt
     2581 gctcgagtcc gtccctgccg ccgaccggcg ctcggtgttc gcgtaccagt tcaccagcca
     2641 ggcgctcggg atggccgtcg gcgccttcgc ggcggggctg gtcgtcgagg tcgacacccc
     2701 gaccgggatg tggccggcct tcgcggcggc gacgctcggc ttcgtcgtga ctgcggcctt
     2761 cctggctgcg gcgacgcgga cggccgccga cctcacagcg gccacccgct ggggtcagga
     2821 ggtctcgaca ggctcgaccg ccggttcagg ctcgaccgcc gaactcgacg gcgacgggtg
     2881 gcggacgtac cggttcctgg cacggaaccg gcaggtccgg ttgctcggac tgctgtcgat
     2941 cgcgctggca gcgggcttct acgctcagtt cgagaccggc cttccggcgt tcgcgctgca
     3001 gtccctgtcg gtcgagccga ccgtcgtcgg aacggctgcc gcggtcaact gcgtggtcat
     3061 cgtcggcctg cagtggctgg tcgtccgact gaccggcagc cagagcggag ccgccctgct
     3121 ggccgtcgtc ggtggcatct gggtcgcgag ctggttgctg ctcgaggtcg ccctgttcgt
     3181 gtctccccag gtcgccggtg tgatgttcgt cgtgacgttc gcgatcttcg ccgtcggcga
     3241 gacgatgtac gcaccgatcc tgagcccgct cacggctgct gtcgcccctg cgggcctggt
     3301 cgggacgacg ctcggtgccc tggcggcgct gcgtaccggg atcagcgcgg tcgggccgct
     3361 ggtggccggc gtgctgttcg cgttcgacct tccccacgtg ttcgtgctcg gccacgtcgc
     3421 gatcaacgcg gcagcgatcg cgctcgccgt ccggctccac caggcgcaga cagcacctac
     3481 ctcagacgtg gatactgttg cgcagggggc accggaccgc tccggcggca cagacgccga
     3541 gccggtgccc gcgtagggcc gcgtccctga cggcgctgcc caggaggagt agacgtgtca
     3601 gaccgcttcc agcccgccac ctaccccgtc ccggccagcg acgccttggt cgagttcatg
     3661 cggaccggtt ggtccgacga ggacgacgtg gtcgcgcgtc tcgatgtcgc gtcgtgggcc
     3721 gagcagcggc gggcgaagct cgtcgagcgg ttcccgggcg agcgcatcat cgtgccggcg
     3781 ggtccgctca agcctcgtgc caacgacacc gactaccggt ttcgcgccga gaccgcgcac
     3841 gtctacctga cgggcaacca gacctcggac gcggttctgg tcatcgacga cggcgaggcc
     3901 accttgttct tccg
//
LOCUS       DATBFZ010000097         3492 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_3338217, whole genome shotgun sequence.
ACCESSION   DATBFZ010000097 DATBFZ010000000
VERSION     DATBFZ010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 3492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3492
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3338217"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..799
                     /locus_tag="VH419_02925"
     CDS             <1..799
                     /locus_tag="VH419_02925"
                     /inference="COORDINATES: protein motif:HMM:NF036686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042600.1"
                     /translation="LRMPTVNWPDVTIVNVGVLTPHGTAGADVEFEVMAGDKVSTRVA
                     QISLAQAGGNPAPPTSPGELRSHAHPSVVDIAAAELEPATLAAIAYASTSSAYVIGPD
                     AERDLTERLRARWSIPVFSTPSSAVDALRKFEVQRLAVVHPSWFGEVHNALGAAYFRD
                     QGFTVVQAELADVQGDPDLVEVDSVVEWVSNHVDRDAEAVFLGGNGFRAAGAVAELEK
                     RLRCLVLESNQVLLWSILSETGSPVSIRGFGRLFHATGSASTSGHYR"
     gene            837..1424
                     /locus_tag="VH419_02930"
     CDS             837..1424
                     /locus_tag="VH419_02930"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY7042601.1"
                     /translation="MFLTMAMSLDGFIAGPDDDAENGPRDARACSEQRNRRERSMLTV
                     GSDGLDGVMRSNRSIPTATVIPVLSYPDVRAAVAWLGVAFGFIERVRIGDGHRAQLSV
                     GEGAVIVADTGGSRRPPDPSVVTHSVMVRVSDVDAQCERALRQDAIIVMEPTEFPYGE
                     RQCTIQDPWGHQWTFSQTLDDVVPEEWGGETVVPA"
     gene            1717..2268
                     /locus_tag="VH419_02935"
     CDS             1717..2268
                     /locus_tag="VH419_02935"
                     /inference="COORDINATES: protein motif:HMM:NF025052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY7042602.1"
                     /translation="MTPAPRLVLLNGPPGAGKTTVAGALKARFSGSARLAVLEHDDFA
                     VMAGAPAEGEASVRTWTSSLHALCSAARVYVDEAWSVLVVVNYGRARKTLLERLLDPV
                     PVRHVLLLPPWSVSRDRALERVSAASSSALPFIGSDAHRRFHEDLRLMAREGQFDEVI
                     DPAESGVQLTCALLSHHLGLVHP"
     gene            3182..>3492
                     /locus_tag="VH419_02940"
     CDS             3182..>3492
                     /locus_tag="VH419_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEY7042603.1"
                     /translation="MPDQPNILIIWGDDIGISNLSCYSDGLMGYRTPNIDRLADEGAR
                     FTDSYGEQSCTAGRAAFITGQNPYRSGLTKVGFPGADIGLRAEDPTIATALKEQGYAT
                     G"
BASE COUNT          589 a         1124 c         1184 g          595 t
ORIGIN      
        1 gctgcggatg ccgaccgtga actggccgga cgtgaccatc gtcaacgtcg gagtgctgac
       61 gccgcacggc acggcggggg cggacgtcga gttcgaggtc atggccggcg acaaggtgtc
      121 cactcgagtc gcccagatct cattggcgca ggctggcggc aacccggctc cgccgacgtc
      181 gccaggcgag ctacgctcac acgcccaccc ctcggtggtc gacattgcgg cagccgaact
      241 cgagccggcc actctggccg cgatcgccta cgcctcgacg agctccgcct acgtgatcgg
      301 cccagacgcc gagcgcgacc tcaccgaaag acttcgcgca cgttggtcca tcccggtctt
      361 ctccactccc tcatcagccg tcgacgcgct tcggaagttc gaggtgcaac ggctcgcggt
      421 cgtgcacccg tcatggttcg gcgaggttca caacgctctt ggggcggcgt acttccgtga
      481 ccaggggttc accgtggttc aagcagagct cgccgacgtc cagggcgacc cggacctcgt
      541 cgaggtcgac agcgtcgtcg agtgggtctc caaccacgtc gaccgcgatg ccgaggcggt
      601 gttcctggga gggaacggct ttcgcgccgc cggggccgtc gcagagttgg agaagcgcct
      661 ccgctgtctt gtcctggagt ccaaccaggt tctgctgtgg tcgatcctgt ccgaaaccgg
      721 cagccccgtg agcattcgag gcttcggccg cctgttccat gccaccggct cggcgtcgac
      781 cagcggccat taccgatgaa ccaccgcaaa ctgcagaaag ggcgagcatg tcccgagtgt
      841 tcttgaccat ggcgatgtcg ctggacggct tcatcgccgg acccgacgac gacgccgaga
      901 acggtccgcg cgacgcgcga gcatgcagcg aacagcggaa ccgtcgcgag cgctcgatgt
      961 tgacggtcgg aagcgacggg ttagatggcg tcatgagatc caatcgctcc attccgacgg
     1021 cgacggtgat accagtcttg agctacccag acgttcgggc tgcagtggcg tggttgggag
     1081 tggccttcgg gttcatcgag cgggtgcgaa tcggtgacgg tcaccgcgcg cagttgtcag
     1141 taggcgaggg tgcggtcatc gtcgccgata ccggcgggag ccgtcgacct ccagacccat
     1201 cggtggtcac gcactccgtg atggtgcggg tcagcgacgt ggacgctcag tgcgagcggg
     1261 cgctgcgaca ggacgcgatc atcgtcatgg agccgaccga gttcccgtac ggcgagcgcc
     1321 agtgcacgat tcaagaccct tgggggcatc agtggacgtt ctcgcaaacg ctcgacgacg
     1381 tcgtgcctga ggagtggggc ggcgagaccg tcgtgcccgc ctagtctcgt cggcgaatgc
     1441 gggtgcttcc atcgaacgtc ggtcacgaaa tccccacgcc tggctgcggc tctagtccgc
     1501 cgtgtcgtcg ggtctcgacg gggtgttctc gcggagatct gtctgacgac agttcgacgg
     1561 tcatccttgt gatccggtga gatcccgccg cgtcgtacgg gcatcacaca tgcggctgaa
     1621 cacatggccg agcaagggct caccggcgtc aagccgggtc agggccaggc ccccaaactg
     1681 ggaagccgga atgtggggcg ctgacacact caggcaatga ctcccgctcc acggcttgtc
     1741 ctgctcaacg gaccccccgg tgccggtaag accacggtgg ccggtgcgct caaagcacgg
     1801 ttttccggct cagcgcgcct ggctgtcctc gagcacgacg acttcgccgt catggctggg
     1861 gcgccggcgg agggggaagc gtcagtgcgc acttggacgt ccagccttca cgccctgtgc
     1921 tcagccgcac gcgtctacgt cgacgaagcc tggtcggtcc tcgtcgtcgt caactacggt
     1981 cgggcccgca agacactgct cgagcggttg ttggatccgg ttccggtgag gcatgttctc
     2041 ttgttgccac cgtggagcgt cagccgcgat cgagcccttg agcgggtttc cgcagcatcg
     2101 tcatccgcgt tgccgttcat cggctcggat gctcaccgac ggttccacga agacctgcgt
     2161 ctcatggcgc gtgagggcca gttcgacgag gtgatcgacc cagctgaaag tggcgtgcag
     2221 ctgacttgcg cactcctgag tcaccacctg ggcctcgtcc acccatagca acgagcgttc
     2281 gaccatttgg acgctgggcc gctggtcagg catgccgatg attggcgggg tgccgatgag
     2341 cgtgcttcgc ttcctgtgcc acttcgagac cccatgcgag ggcggaggtt ctgatgtgga
     2401 gcacccgcaa ctagtccgag ctgacgtacc ggaccgacac cacgtcgttc tggtcgcccc
     2461 atcgcaacga gaaggtccag cagcccgccg ctggcaagtt gatgaccgag ggcctcgaag
     2521 atccgggtgt cacctgacct tcgacccggc gttgcacatg aaggtcagag ccgttcaacg
     2581 atgcagagat cagcaggtcc gccgtgctgg ccgtgcgggc gggccgggac aacatccaca
     2641 agatcttgtt acggcgatca cgaagcggcg gtgacgtcag cggctctggc cagccgaagg
     2701 aggcgcccac aacgttgtgc cgcacaccca caaggcgccg ttggacgccg acgggacatg
     2761 gcaacgagac cgaagcatgg ggagtcgcgg aagtccgggt ctccggcgtg atcaaggaca
     2821 ccgctgtgga cggcgacgaa ggcgtggcgg cttcctgcgg atccggcgac cccgtgcagg
     2881 acgtgaggac aagcaggatc aggacaggtg tgaggagcgc cctcatcgga agatggtacg
     2941 tcgctgtcgg ccctgaggtg cttcgcgacg ccgagcgccg gcgactcggg tggacggtct
     3001 gcaacgcccg ccttcgccgc ctcctgcgcg tccgggagcc gcgtcttgcc ggcccgtgtc
     3061 ggactcgggt gcgaccgagt caagtcgtag atcgcgtcgc cttggacttc acccggagta
     3121 ggtgaatcgg tctttgtgcg atggtggtgg gctgaacgga cggactctgg aggtgtggta
     3181 ggtgccggac cagccgaaca tcctgatcat ctggggcgac gacatcggga tctcgaacct
     3241 gagctgttac agcgacgggc tgatgggcta ccgcacgccg aacatcgatc ggctcgcgga
     3301 cgagggcgcg cggttcaccg actcctacgg cgagcagagc tgcaccgccg gccgcgccgc
     3361 cttcatcacc ggccagaacc cgtaccggtc cggcctcacc aaggtggggt tcccgggcgc
     3421 cgacatcggg ctgcgcgccg aggatccgac gatcgcgacc gcgctcaagg agcaggggta
     3481 cgcgaccggg ca
//
LOCUS       DATBFZ010000098         2122 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_715766, whole genome shotgun sequence.
ACCESSION   DATBFZ010000098 DATBFZ010000000
VERSION     DATBFZ010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_715766"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..834
                     /locus_tag="VH419_02945"
     CDS             <1..834
                     /locus_tag="VH419_02945"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEY7042604.1"
                     /translation="AIELVHASALVHDDVMDNSETRRGAPSAHREFSRLRRQNPRADA
                     SERFGTSAAILLGDLLLSWSDELLSQSGFKPRAIARSRVYFDKLRTEVVAGQYLDVLA
                     QTSQTSRASDAMRVVRYKSAKYTVERPLQFGAALAKAERPLLTALSSYGIPLGEAFQL
                     RDDVLGVFGVSDVTGKPSGDDIRDGKRTLLIARAFDKATAAQHSVLAKNLGRGDIDDA
                     GIRAVQDVLRETGALAAVEIVIAELTAAARSALDHADIEADEPRRALLMLTERATQRA
                     H"
     gene            complement(1000..1314)
                     /locus_tag="VH419_02950"
     CDS             complement(1000..1314)
                     /locus_tag="VH419_02950"
                     /inference="COORDINATES: protein motif:HMM:NF021378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HEY7042605.1"
                     /translation="MSTITDTTSAMTSLAAQHAPAVVTFAANGPGDGDGPGWWVIFPI
                     TWLLVVATVITVVVLGVRRRGRLSGRRAGERRLAERYASGEIDESEYRARLATLRQIG
                     SD"
     gene            1511..>2122
                     /locus_tag="VH419_02955"
     CDS             1511..>2122
                     /locus_tag="VH419_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020390711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY7042606.1"
                     /translation="MTQHAPATEPLPVQTTSLTKRYGSIVAVDALDLRVRPGEVYGFL
                     GPNGAGKTTTLRMLLGLVRPTAGQVRLFGAPPGPGQLTHVGALVEGPAFYPYLSGRDN
                     LRTFAAHADVRRSRVDEVLETVELSARARDRYATYSLGMKQRLGLAAALLKNPSLLIL
                     DEPTNGLDPAGMADMRVTIRSLASQGCTILLSSHLLAEVQQVCD"
BASE COUNT          334 a          753 c          713 g          322 t
ORIGIN      
        1 gccatcgagc tcgtccacgc cagcgctctg gtccacgacg acgtgatgga caactccgag
       61 actcgccgtg gcgctccctc tgcgcaccgg gagttcagcc ggctgcgtcg ccagaacccg
      121 cgcgccgacg cgtccgagcg cttcggcacc agtgccgcga tcctgctagg cgacctcctg
      181 ctgtcgtggt cggacgaact gctgagccag agcgggttca agccgcgcgc cattgcccgc
      241 agtcgggtgt acttcgacaa gctccggacc gaggtcgtcg ccggccagta cctggacgtg
      301 ctcgcccaga cgagtcagac cagtcgcgcg tctgacgcca tgcgggtcgt tcgctacaag
      361 tcggcgaagt acaccgtgga gcgaccgctc cagttcggcg ccgcgctcgc gaaggcggag
      421 cggccactgc tgaccgcgct gtcgtcgtac ggcatcccgc tcggcgaagc cttccagctt
      481 cgcgacgacg tgctgggcgt gttcggtgtg agcgacgtga ccgggaagcc gagcggggac
      541 gacatccgcg acggcaagcg caccctcctc atcgcccgag cgttcgacaa ggccactgca
      601 gcgcaacaca gtgtgctcgc gaagaacctc ggccgcggcg acatcgacga tgcagggatc
      661 cgggctgtgc aggacgtgct ccgcgagacc ggtgcgctgg cggcggtcga gatcgtgatt
      721 gcggagctga cggcggccgc gcgctcggcg ttggaccatg cggacatcga ggccgacgaa
      781 cctcgtcggg cgctgctcat gctcaccgag cgagcgaccc aacgagcgca ctgacgcccg
      841 gccgctcatc gagcctgcca gggccactca ggtcgccgaa gcgccttgtc gagctgcgcc
      901 acggcaggcc tgcacggcag atgcctggtc tgcatcgtgg gtttgcgatg tttacacgcc
      961 ttttgggctg taaacatcgc aaacccccct cgcgacggct cagtcggagc cgatctggcg
     1021 gagagtggcg aggcgggcgc ggtactccga ctcgtcgatc tccccggacg cgtaccgctc
     1081 ggccagccgt cgctcgccgg cgcgccgtcc ggagaggcgg ccacggcgtc gtacgccgag
     1141 caccacgacg gtgatcacgg tcgcgacgac gagcagccag gtgatgggga agatcaccca
     1201 ccatcccggg ccgtcgccgt cgccggggcc gttggcggcg aacgtcacca cggccggcgc
     1261 gtgctgggcg gccagtgagg tcattgcaga ggtcgtgtcg gtgatggtgg acatcgtggt
     1321 tctccttcgg aagctgactg tcgatgtcca ccaggttcgt tcgccaccgc cgtcgcgaca
     1381 tcggtcagga gacgacacct cgaggccgct tcgggacgtg cgtaccgcca ccgccgtacg
     1441 tcgcacgacg tacacacgaa gacgcccgag agccgacgtg tcagccccgc tcggcgccga
     1501 ctctggaggc atgacacagc acgcacctgc caccgagccg ctccccgtcc agacgacgtc
     1561 actcacgaag cgctacggat cgatcgtcgc cgtcgacgcg ctggacctgc gggtgcggcc
     1621 aggcgaggtc tacgggttcc ttggacccaa cggtgccggc aagaccacga cgctccgcat
     1681 gctcctcggg ctcgtccgtc cgacggcagg ccaggtgcgg ctcttcgggg cacctcccgg
     1741 accaggtcag ctgacccacg tcggcgcact ggtcgaggga ccggccttct acccgtacct
     1801 ctcggggcgg gacaacctcc gcaccttcgc cgcccatgcc gacgtacgcc ggtcccgtgt
     1861 cgacgaggtg ctggagaccg tggagttgtc ggctcgggca cgagatcgct atgcgacgta
     1921 ctcgctcggc atgaagcagc ggctgggcct cgcggccgcg ctgctcaaga atccgtcgct
     1981 cctgatcctc gacgagccga ccaacgggct cgacccggcc ggcatggccg acatgcgcgt
     2041 gacgatccgc tcgctcgcct cgcagggctg cacgatcctg ctgtcgagcc acctgctcgc
     2101 cgaagtgcag caggtctgcg ac
//
LOCUS       DATBFZ010000099         1570 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_1372476, whole genome shotgun sequence.
ACCESSION   DATBFZ010000099 DATBFZ010000000
VERSION     DATBFZ010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1372476"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..220)
                     /locus_tag="VH419_02960"
     CDS             complement(65..220)
                     /locus_tag="VH419_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042607.1"
                     /translation="MAGGKSMPPCTLIQVLGLDDDVCCGRCAEEIDDFVVLLSRHTAT
                     RAPALAT"
     gene            complement(345..>1570)
                     /locus_tag="VH419_02965"
     CDS             complement(345..>1570)
                     /locus_tag="VH419_02965"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY7042608.1"
                     /translation="GHSVRMINGEANAHMVDSIQGLRTIVAFNHGADQARDIQGTSSR
                     LNAAKQSLLRWQALQGSLVEALIGIGGLAVLTMGASLVSRGTMGRYDLPLVTLLAMSS
                     FLPVVTIVTVAKELVQTLASGRRYFAIEDEPVPVRDGPGASTAVGAAQGLPVAFQAAT
                     FRYNPGERPALDNVSFEAPAGGTVALVGRSGAGKTTLAHLLLRFWDPQSGQILIGGED
                     ARHFELDELRRRIALVAQDTYLFHTSLRENIRLGRPDASDEDVLDAARAANVEEFALA
                     LPNGYDTVVGERGMQLSGGQRQRVAIARAVLKDAPILVLDEATSHLDAINEAQVHEAL
                     SRLMRGRTTLIIAHRLSTIRNADRIVVLDAGRAVEQGTHGELLERDGLYSHLIASQLF
                     LPRTRATASGVGGRA"
BASE COUNT          274 a          552 c          497 g          247 t
ORIGIN      
        1 tggtgagaat cgtgttcaag tgctccagga caccgggttt cgcctgcacg actcctcctt
       61 ctggtcaggt cgccagcgcc ggcgcccgag tggccgtgtg ccggctcagc agcacgacga
      121 agtcgtcgat ctcctcggcg cagcgcccac aacatacatc gtcgtcgaga ccgagcacct
      181 gaatcaaggt gcacggtggc atgctcttac cccctgccac ctcgaccaca tcctgctcgg
      241 tgagcccctt acagagacac atatacatgc ggctgactcc ccccggagaa atggttgcta
      301 caggtcaccg acgacaaaag actcaccaaa ggagcatgaa cggctcacgc ccggcccccg
      361 acaccggacg cggtcgcacg ggtgcgcggt aggaagagct gcgacgcgat gaggtgcgag
      421 tacagcccat cgcgctccag cagctctccg tgggttccct gttccacagc ccgaccggcg
      481 tcgagcacga cgatgcgatc ggcattgcgg atcgtggaca gccgatgcgc gatgatcagg
      541 gtcgtgcgcc cccgcatcag ccggctcagc gcctcgtgga cctgggcctc gttgatggca
      601 tccaggtgcg aggtcgcctc gtcgagaacc aggatgggcg catccttcag gacggcgcgc
      661 gcgatcgcca cgcgctggcg ctggccgccg gagagctgca ttccacgctc gcccaccacg
      721 gtgtcgtacc cattgggcaa cgcaagcgcg aactcttcga cgttggccgc ccgggctgca
      781 tcgaggacgt cctcgtcgct ggcgtcggga cgacccaggc ggatgttctc ccgcagtgag
      841 gtgtggaaga gataggtgtc ctgcgcgacc agggcgatgc ggcgtcgtag ctcgtcgagc
      901 tcgaagtgcc gggcatcctc ccctccgatc aggatctgtc ccgactgcgg gtcccagaag
      961 cgaagcaaca ggtgcgcgag cgtggtcttg cccgcgccag agcgaccgac gagcgccacc
     1021 gtgccgcccg cgggcgcttc gaacgaaaca ttgtccagcg ccggccgctc gccggggttg
     1081 tagcggaagg tcgccgcctg gaacgccacc ggcaatccct gcgcagcgcc gacggccgtg
     1141 ctagctccgg ggccatcccg gaccggcacc ggctcgtcct cgatcgcgaa gtaccgtcgc
     1201 cccgaggcca gcgtctgcac cagctccttg gcaaccgtca cgattgtcac caccggcaag
     1261 aacgacgaca tcgcgagcag cgtcacgagc gggagatcgt agcgtcccat ggtgccccgc
     1321 gacaccaggc tcgcacccat cgtcaacacg gccagcccgc cgatcccgat cagcgcctcc
     1381 accagcgacc cctgaagcgc ctgccagcgg agcaggctct gcttcgcggc gttcaaccga
     1441 ctgctggtgc cctggatgtc gcgggcctga tcggcaccgt ggttgaaggc gacgatcgtg
     1501 cgcagcccct ggatgctgtc gaccatatgg gcgttggctt cgccattgat catgcggacc
     1561 gagtgtccca
//
LOCUS       DATBFZ010000100         2524 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_4625782, whole genome shotgun sequence.
ACCESSION   DATBFZ010000100 DATBFZ010000000
VERSION     DATBFZ010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4625782"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="VH419_02970"
     CDS             complement(<1..264)
                     /locus_tag="VH419_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020575011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY7042609.1"
                     /translation="MSPVRLLVVDDHPVVRDGIVGMVASDPEIEVVGEASDGAEAVSL
                     ARVLEPDVVLMDLRMRGMDGVAATRRLTSLGVPTRVVILTTFDA"
     gene            complement(261..1373)
                     /locus_tag="VH419_02975"
     CDS             complement(261..1373)
                     /locus_tag="VH419_02975"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEY7042610.1"
                     /translation="MDPRWRTVLTVTPYLVLGMLAAFALGVEWGAWQQLVPTLVLCAT
                     VAAWILVLRDLALPWRDRPAAIAVFMAGLIALNLTLVLRDSWFGFLTVATFSFAYSIV
                     PWPWKLLAVGATATVAGLAQSSDLGHDRAGVTETVAVVALNVVVMCGLAWGLRLAQQQ
                     QVRMATEVERARLAREIHDALAQGFAGIVTQLQAGEQAPDEAARRRHTDAALALARDG
                     LAEARRSVQALRPTALDTSRLPEALENVTRTWSARTGIVVQVDTSGDGRALPTDAEVA
                     LLRTVQEALTNIERHAGAHRVVVSLRSGADGTHLEVRDDGRGFDPSEQPALPGRPEEG
                     GYGLVAMRERIESIAGELVVESRPGHGTAVLAEVPR"
     gene            complement(1386..1817)
                     /locus_tag="VH419_02980"
     CDS             complement(1386..1817)
                     /locus_tag="VH419_02980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042611.1"
                     /translation="MTETLTHTRPASDAERRTAFRPVLTLLAGFLVVSAAMEAVLVAL
                     TTTNGTVDIAIWIRCSLVLASSIVLLLLAVVAVRGSRPAWLRLRIISPIVVAAVVVVV
                     SIPGFLPDWVRVEQGVCGALVLGVAILVNLPRARALFPRAE"
     gene            2073..>2524
                     /locus_tag="VH419_02985"
     CDS             2073..>2524
                     /locus_tag="VH419_02985"
                     /inference="COORDINATES: protein motif:HMM:NF016840.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEY7042612.1"
                     /translation="MIANDAERALLLAHLEGHRNHALGILDGLDEPTLRRAAVPSGWT
                     CLGMIRHLTMDEERFWFRAVVAGDREAIAFFDGGPDTWQVGADETVADVLAAYRSEIE
                     RSNAIIAGTSLDAAPAWWPPDLFGDWRLNSLREIMLHMISEVACHAGHL"
BASE COUNT          424 a          868 c          905 g          327 t
ORIGIN      
        1 ggcgtcgaag gtcgtcaaga tcacgacacg agtcggcacg ccgaggctgg tgagcctgcg
       61 ggtcgcggca acgccgtcca tcccgcgcat cctcagatcc atgaggacga cgtccggctc
      121 gagcacgcgc gcgaggctga ccgcctcagc gccgtcggac gcctcgccga ccacctcgat
      181 ctccgggtcg gaggccacca tgccgacgat gccgtcgcgc acgacagggt ggtcgtcgac
      241 caccagcagc cgcaccgggc tcacctcgga acctcggcga gcaccgcggt cccgtgccct
      301 gggcgcgact cgacgacgag ctcgcctgcg atcgactcga tgcgctcccg catcgcgaca
      361 aggccgtagc cgccttcctc cggacgaccg ggcagcgccg gctgctccga ggggtcgaag
      421 ccgcggccgt cgtcgcgcac ctcgaggtgt gtgccgtccg cacccgaccg cagagagacc
      481 acgacgcggt gagcgccggc gtgccgctcg atgttcgtga gcgcctcttg gacggtccgc
      541 agcagagcga cctcggcatc ggtcggcagg gcacggccat cgccagaggt atccacctgg
      601 accacgattc cggtgcgcgc cgaccacgtc cgcgtcacgt tctcgagggc ctctggtagc
      661 cgactcgtgt cgagggcggt gggccgcaga gcctgcaccg agcgtcgtgc ctcggcgagg
      721 ccgtcacggg cgagggccag cgcggcatcc gtgtggcgcc gcctggcagc ttcgtccggc
      781 gcctgctcgc cggcctgcag ctgagtcacg atgcctgcga acccctgcgc gagagcgtcg
      841 tggatctccc gcgcgagccg cgcccgctcg acctcggtcg ccatccgtac ctgctgctgc
      901 tgggccaggc ggaggcccca agcgaggccg cacatgacga caacgttgag cgccacgacg
      961 gcgacggtct ccgtgacacc ggcgcgatcg tgccctaggt ccgagctctg cgccagcccg
     1021 gcgaccgtcg ccgtggcgcc caccgccagc agcttccatg gccagggcac gatcgaatag
     1081 gcgaaggaga aggtcgcgac cgtgaggaac ccgaaccagc tatcacggag caccagcgtg
     1141 agattcaacg cgatgagtcc agccatgaag acggcgatcg cagcgggacg gtcccgccac
     1201 ggcagcgcca ggtcacgcag gacgaggatc cacgcggcga cggtcgcgca caggacgagg
     1261 gtgggaacga gctgctgcca tgcgccccac tcgacgccga gtgcgaacgc ggcgagcatc
     1321 cccagcacga gatagggcgt gaccgtgagg acggtccgcc agcgcgggtc catcacgtgg
     1381 tgaccctact ccgctctggg gaagagagcc cgggctcgcg gcaggttgac aaggatggcg
     1441 acgcccagca cgagtgcgcc gcacaccccc tgttcgacgc gcacccagtc gggcaggaac
     1501 ccgggaatgg agacgacaac gacgacggcg gcgacgacga tcgggctgat gatccgcagc
     1561 cgcaaccacg cgggacggga gcctcgtacg gcgacgacag ccagcaggag caggacgatc
     1621 gagctggcca gcacgaggga gcagcgaatc cagatcgcga tgtccacggt gccgttcgtg
     1681 gtggtcagcg cgacgagcac ggcctccatc gcggcgctga ccacgaggaa gccggcgagc
     1741 agggtgagca ccgggcggaa ggcggtccgg cgctcagcgt cggacgcagg acgggtgtga
     1801 gtgagtgtct cggtcatggc tcaagccttc cgggcggcag ccgacagcgg atccaccgaa
     1861 cggtccgggt gcggcaccga tcggttggga tctgtcggca ccaccacgag cgcccaagcc
     1921 ctgccggctc atacccggca gggctcaagg ggcgtgcgga gacgtgcgtc cggggtcagt
     1981 ctgtgtgtga cctcgcccgc gagacggcaa cgacacgccg caacgtcgaa cgatgctgcg
     2041 ctgcgggcgg gctgcgggag atcgtggaga acatgatcgc gaacgacgct gaacgggccc
     2101 tgctgcttgc ccatctggag ggccaccgca accatgctct cggcattctc gacgggttgg
     2161 acgaaccgac gctgcgacgt gccgcggtgc cgtcgggctg gacctgcctc ggcatgatcc
     2221 gacacctgac gatggacgag gagcgtttct ggttccgggc ggtggtggct ggcgaccgcg
     2281 aggcgatcgc gttcttcgac ggcgggccgg acacctggca ggtcggcgcg gacgaaacgg
     2341 tggccgacgt gctggcggcc tatcggtcgg agatcgagcg ctcgaacgcg atcatcgcgg
     2401 gcacgtcgct cgacgcggcg ccggcctggt ggccgccgga cctgttcggg gactggcgat
     2461 tgaacagcct gcgggagatc atgctgcaca tgatcagcga ggtcgcctgc cacgccggcc
     2521 acct
//
LOCUS       DATBFZ010000101         9236 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_4954465, whole genome shotgun sequence.
ACCESSION   DATBFZ010000101 DATBFZ010000000
VERSION     DATBFZ010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 9236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9236
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4954465"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(138..281)
                     /locus_tag="VH419_02990"
     CDS             complement(138..281)
                     /locus_tag="VH419_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042613.1"
                     /translation="MNPLNLYLAHHEIDRRLRAVDHHRRIRWGRESSTATTHTYLAGF
                     AQP"
     gene            complement(416..622)
                     /locus_tag="VH419_02995"
     CDS             complement(416..622)
                     /locus_tag="VH419_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042614.1"
                     /translation="MLPILIFESAWKLLWLSVVALPLAATDDLDPTTSEVVVNCSLVV
                     VVMAVIPWRYVWDTYVRAPGDRWR"
     gene            648..941
                     /locus_tag="VH419_03000"
     CDS             648..941
                     /locus_tag="VH419_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008358939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HEY7042615.1"
                     /translation="MPKGYWVSVYRTISDPEKLAAYNKLAAPAVKAGGGRTFVRGGRV
                     VAHDAGVAERTILVEFDSFEQAVAAHASAGYQEALVALSDGVERDFRIVEGID"
     gene            1054..1344
                     /locus_tag="VH419_03005"
     CDS             1054..1344
                     /locus_tag="VH419_03005"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEY7042616.1"
                     /translation="MAIYSIWESTFPPAHAQDGRRLTEAIWEDMIQYNGYLAHELLVD
                     ADDPGHLLVVSRWSSRERADAVLHDYADNPKARAANRLATEPRRRFVALGTS"
     gene            complement(1664..1861)
                     /locus_tag="VH419_03010"
                     /pseudo
     CDS             complement(1664..1861)
                     /locus_tag="VH419_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010078638.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2059..2529)
                     /locus_tag="VH419_03015"
     CDS             complement(2059..2529)
                     /locus_tag="VH419_03015"
                     /inference="COORDINATES: protein motif:HMM:NF016840.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEY7042617.1"
                     /translation="MELLEHFLDAQREAVLGKTEGLDRAQMEQMHPPSTLNLAGLLYH
                     LSLAEEDWMEIHFAGRPDRAPYVDANWDADPDWQFRAPREWEPEQLRQRYREACSRSR
                     AVVRAASNLEQSSTKPVHGQHFTLRWALLHLIEETARHAGHADMLRESIDGSVG"
     gene            complement(2960..3523)
                     /locus_tag="VH419_03020"
     CDS             complement(2960..3523)
                     /locus_tag="VH419_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012919589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEY7042618.1"
                     /translation="MADNPPDYELADRMALTKPAQVKAIGHPLRTTILQLLHERAATV
                     TELAAAVERPKSTVAHHVDVLTRNGLLQVVRTRKVRAIEERFYGRTARMFHVAAEPSP
                     AGEVMPGDFNDFEVAARESAAAFEQGKLWGFIRHARISKADASQFWDRMARLVDEFDL
                     LPRSGETMYGFAIGIYPTDHPILPTAE"
     gene            3591..4160
                     /locus_tag="VH419_03025"
     CDS             3591..4160
                     /locus_tag="VH419_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020387720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEY7042619.1"
                     /translation="MAEVLLYHHIQGLTDGVRAFADDLREAGHTVHAPDLFDGRTFGS
                     IEEGFGFARETGLDAIGERGAAAADELDPGLVYAGFSFGVTIAQRLAQTRPGARGALL
                     MYSCIPIAEFGETWPDGVPVQIHGKEDDEFFDEDLPAARELADSTADAELFVYPGEQH
                     LFADSSLAAYDPEAAALLIERVRRFLASI"
     gene            4334..5008
                     /locus_tag="VH419_03030"
     CDS             4334..5008
                     /locus_tag="VH419_03030"
                     /inference="COORDINATES: protein motif:HMM:NF017771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 family protein"
                     /protein_id="HEY7042620.1"
                     /translation="MGKFDQPTAHPSITDRETFDAALSEQVGREKEVTRHNDRVSAAR
                     RRLPMVEVNDYVFAGPDGPLALTELFADRYLLTVQNFMFDPGWDDGCPSCTWATDNLP
                     ANMDRLAEEGIAFAMISQAPIRKLETWRVKRGWDHLWVSSFDTSYHYDWGWTQTNDQG
                     QEGQLPGYSYYLLRDGTPYLTYMTTARGTEAILPTAQIMDRTCYGRQQDWEDSPEGWP
                     QYPTYG"
     gene            5065..5640
                     /locus_tag="VH419_03035"
     CDS             5065..5640
                     /locus_tag="VH419_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020578202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEY7042621.1"
                     /translation="MTATYTFDVFSSLDGYGAAGGDWTGYWGKQGPELLEHRLTLYGT
                     EQRMVFGANTYRLFAQMLASSTEDSDVRDPWVTRMWRQPATVVSTSLEGPLDWPDATL
                     VSGDAVDIVARLKEESDVSLRSHGSLSMNRALMAAGLVDRVQVTLFPVITGQTGLDPI
                     FQGAADFDLELVESRTLDGHIQELIYRPSLH"
     gene            6334..8502
                     /locus_tag="VH419_03040"
     CDS             6334..8502
                     /locus_tag="VH419_03040"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEY7042622.1"
                     /translation="MERLSSFVLAHRRWVFAFWVVMFVAGVAAAGAVPNRLSYDFSLP
                     GQQGYETEQKIIASYQGANAQAAYVPVVTVPDGQTVAGSAADVKAVFDALGRIPTLRV
                     VDYSQTHDKGFITSDGRTTFGVIYEPQPNGFVDPNQKQIEDTFNAQLEQHGFTGGLTG
                     YQQLSSGGGSDSGPSVLEETLLGALGALAVLAFVFASLLALVPLLIAAVSILTTFLIV
                     LLLTTFTDVSFVVQFLIALVGLGVAIDYSLLVVSRWREEIAHGNDNHEAVRISVRTAG
                     HAVFASGVTVAISLIALVVIPVPLLQSMGYGGMLIPLCSVAVVLTLLPAMLAAVGPRV
                     DIPHIRKEGTASRGWTAWARMIVRRPWLFGGAAIIVLAILIAPLRHIQFGQSSTASQG
                     TSSPAYQTLQTLIDGDVGTGVTTPIVLLADNENDAAAIASAAGKVEGIRVAAAYAPGT
                     DGRTAIDVIPDDETVNSSSVEVVDRVTAATESLPGYIGTAGLGATIVDYRHAVYDNFP
                     YVIGLIAFITFLLLMRTFRSVLLPIKAVVLNLISLGAVMGLATWFWQEGNGSSAVFGI
                     EATGAMTFWLPPLIFAFLFGLSMDYEVFILTRMREEYDRSGSTEAAVVRGIGRTGRLV
                     TSAALILFFAFAALASAPGTDIKVFATCLGVGILIDATIVRAVLVPSLVVLFGRWNWW
                     LPAWIARILRVEPSPLHEHGEPGLVDVDLSEPHVPSSASD"
     gene            complement(8936..>9236)
                     /locus_tag="VH419_03045"
     CDS             complement(8936..>9236)
                     /locus_tag="VH419_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042623.1"
                     /translation="AIAYLHGRRGDRAARQHWLGITAAVRGVPRPLATEATGYGEVFE
                     AALALQHGRSSDAAAALARVPADSWYGAMFTRWRTQLAAELDTIGQAQPSRGTSS"
BASE COUNT         1561 a         3095 c         3011 g         1569 t
ORIGIN      
        1 ccgcctgctc ggtctccctt ccgtgcagtg ccacgctcgg agactaactg ctcacgggac
       61 cgcgatctgt cgctcaccct gacaggtgaa gggccccgac gaggggcctc accgtggact
      121 gggccgacgg gcgggactca cggctgggcg aaaccggcca agtaggtgtg ggtggtggcg
      181 gtcgaggact cacgacccca gcggatgcgt cggtggtggt cgaccgcgcg aaggcggcgg
      241 tcgatctcgt ggtgggccag gtagaggttg agcgggttca tggcaaggct cccttcgggt
      301 tcggtggagc caccaggttg ccgggccgcg ggggtcggtc tcgccgggaa gatcgccagg
      361 ttcagcggca ggcactccag ttcacggtgg tgcgccccct gcggagactc cgcactcagc
      421 gccaccggtc accgggtgct cgcacgtagg tatcccacac gtaccgccag gggatgacgg
      481 ccatgacgac gacgaccagc gagcagttca ccacgacctc ggaagtcgtg gggtccaggt
      541 cgtcggttgc cgccagggga agggccacga cgctgagcca gagcagcttc cacgcagact
      601 cgaagatcag gatgggaagc atgcactcat cccacactgg aggttccatg cccaagggct
      661 actgggtcag cgtttaccgc accatctcag accccgagaa gctggctgcc tacaacaagc
      721 tcgccgctcc agccgtcaag gccggaggcg gccggacatt cgtccgcggc ggtcgggtcg
      781 tggctcatga cgccggagtc gccgagcgca ccatcctggt cgagttcgac tccttcgagc
      841 aggccgtcgc ggcacacgcg agtgcgggct accaggaagc gttggtcgcc ctctccgacg
      901 gcgtagagcg cgacttccga atcgtcgaag gcatcgacta acgcgctcga agggcgccac
      961 cccgggagcg tcgtccgcct caaacccacc gcaacgccag gacaggcgga accaacgaac
     1021 agttagccac ccctaccaca aggaggactg tcaatggcca tctactcgat ctgggagtca
     1081 acattcccac cggcgcacgc ccaagacgga cgaaggctca ccgaagccat ctgggaagac
     1141 atgatccagt acaacggcta cctagcgcat gaactgctcg ttgacgccga cgaccccggc
     1201 cacctactgg tcgtcagccg atggtccagt cgtgaacgcg ctgacgcagt gctgcacgac
     1261 tacgccgaca accccaaggc gcgggctgcc aatcgattgg ccaccgagcc acggcggcgc
     1321 ttcgttgctc tgggaacatc ctgatccgag cgcgcggcgg ctgaccttag aatcgtgcgc
     1381 cctcgtccca ccagatgcga ggcggcgacg gctcctaggc tgaacgtctc cggcttcggg
     1441 acgaagtcgt ggtaccgcaa cgttctcgcc gatccatccg tgcacctctc gtgcggcctg
     1501 cggaaacgcg cgagagccac ggcccgcccg atgaccgaag aggagtccgc cgacaccctc
     1561 cgaggctatg ccgaacgcca ccccaaggca tgggcgaacc tgcgttcgac caggtcaagt
     1621 ccgctggcgt ggtgtacgag gggtgatcct tcgtcgttgt ggttcaggct gtgatggcca
     1681 ggatggtcag tcggtcactc acctccttgg ttgcgaggtc gacggcttgg gcgcgggtga
     1741 ggacctcgag tccgaggtag cggcggcctt cggcccattc gtcgtgttgt tcggccagga
     1801 cggcgccgac cagtcggatg atcgcgggtc ggccgggaaa gatgccgacc acgtcggtgc
     1861 ggatgagcgg gcagcgcaac caggacggct agcagagaaa ggctgatata gagggccatc
     1921 gtcatcgtct cgcgaatcca ctcgcgttcg cgcgctacgc gaccgaccac cacccgagtc
     1981 tatggatcac cagccactga gcggatgcgt cgggaagcta actgccgctg tccgtgcgca
     2041 gccgagcggt cgtcaggcct agccgacact cccgtcgatc gactcgcgca gcatgtccgc
     2101 atggccggca tgacgagctg tttcctcgat gagatgcaac agagcccagc gcagcgtgaa
     2161 gtgttggcca tggacaggtt tcgtcgagga ctgctcgagg ttcgacgcgg cgcggaccac
     2221 cgcccggctc cgactgcacg cctcccggta acgctggcgt agctgctcgg gctcccactc
     2281 gcgcggagcg cgaaactgcc aatcgggatc cgcatcccag ttggcatcga cgtagggtgc
     2341 ccggtccggc cggcccgcaa agtgaatctc catccaatct tcctcagcga gcgacaggtg
     2401 gtagagcaat ccagccagat tgagcgtgga cggaggatgc atctgttcca tctgcgctcg
     2461 atcgaggcct tcagtcttgc cgagcacggc ctcgcgctgg gcgtccagga aatgctcgag
     2521 caactccacc tcggagccac ttcgatcagg ttcagtcctc acccgtgcag tctagaaaag
     2581 cgtatgtctc ctcgagccaa gcgcggacgg acccaacccc tgacctcctc ccctgagcaa
     2641 gccggacagc cgcccgagtg ctctcctcct cgccctccac aactcgagta gccgcggtcg
     2701 atgtggttgc cccagcggac accagggcgc cagtacaccg gtgatcgcga tgatccccgc
     2761 gacgacgaac accaccaagc tgcacttcat cagctcatgt gccaaccgtt gcgcaactcc
     2821 tcaagcgtca cagccacatg gtgtctggcc ttggtcggtg ccgggatcgt gtcggcagga
     2881 cgatccatcg ctacgacgcg ggcactaccc ggcacgcggg gcgcacgctc gtatgcccgc
     2941 gactgtcgag gggagtgcgt cactcggctg tcggcaggat cggatggtcg gtggggtaga
     3001 tgccgatcgc gaacccgtac atggtttcgc cggaccgcgg cagcaggtcg aactcatcga
     3061 ccagtcgggc catccggtcc cagaactgag aggcgtcggc cttcgagatg cgcgcatgcc
     3121 ggatgaagcc ccagagcttc ccctgctcga acgccgccgc ggactcgcgt gcagctacct
     3181 cgaagtcgtt gaagtcgccc ggcatcactt ccccagcggg tgacggctcg gctgccacgt
     3241 ggaacatgcg tgcggtgcgc ccgtagaagc gctcctcgat cgcccgcacc ttccgcgttc
     3301 gcaccacctg gagcaggccg ttccgagtga gcacgtccac gtgatgggca accgtgctct
     3361 tggggcgctc gaccgcggcc gcgagctcgg tgacggtcgc ggcccgctcg tggaggagct
     3421 gcaggatcgt ggtgcgcaat gggtggccga tggccttcac ctgggccggc ttggtcagcg
     3481 ccatccggtc ggctagctcg taatccgggg ggttgtccgc cacagcggaa cagactagca
     3541 tcgcggaatg ttccaaacta ttcgatcaat ggacgagggt gggtgctgac atggccgagg
     3601 tgctgctgta ccaccacatc cagggcctga ccgacggagt gcgggccttc gccgacgacc
     3661 tgcgcgaggc cgggcacacc gtgcacgcac ccgatctttt cgacggccgc acgttcggca
     3721 gcatcgagga gggtttcggc ttcgcgcgtg aaacagggct cgacgcgatc ggtgagcgcg
     3781 gtgccgctgc ggccgacgag ctcgaccccg ggcttgtcta cgccggattc tcgttcggcg
     3841 tcaccatcgc ccagcggctc gcccagaccc ggcctggtgc ccgcggcgca ctgctcatgt
     3901 actcctgcat cccgatcgcg gagttcggcg agacgtggcc cgacggggtg ccggtgcaga
     3961 tccacggcaa ggaggacgac gagttcttcg acgaagacct gccagccgca cgcgaactcg
     4021 ccgactccac ggcggatgcc gaactgttcg tctaccccgg tgagcagcac ctcttcgccg
     4081 actcctcact cgcggcctac gaccctgagg ccgccgcgct gctcatcgag cgagtgcgga
     4141 ggttcctcgc ctccatctga cggcacggca ttaccagtag gcccaggctc cgtccataca
     4201 tcggcacgaa cgcgcaaggt cggcgccgat gagcggatgt catgcctttg gtcgtctcgc
     4261 cgcgtggttg tccgaggaac gggtacttgt cgactgacag accgacccgg acgagaacgc
     4321 cggagaacag ttgatgggca agttcgacca gccgactgcg cacccctcga tcaccgaccg
     4381 tgagacgttc gacgccgcac tatccgagca ggtcgggcgt gagaaggagg tcactcgtca
     4441 caacgaccgg gtctccgcgg cacggcgcag gttgcccatg gtcgaggtca acgactacgt
     4501 ctttgccgga cccgacggac ccctggccct gaccgagctg tttgccgacc gctacctgct
     4561 cacggtgcag aacttcatgt tcgaccctgg ctgggacgac ggctgtccga gctgtacctg
     4621 ggccacagac aacctcccgg ccaacatgga tcggctcgcc gaggagggga tcgcattcgc
     4681 gatgatctcg caggcaccga tccggaagct tgagacctgg cgggtgaagc gaggctggga
     4741 ccacttgtgg gtctcctcgt tcgacacgtc ctaccactac gactggggct ggacccagac
     4801 aaacgaccaa ggtcaagaag gccaactgcc cggatactcg tactacttgc tgcgggacgg
     4861 gacgccctat ctgacctaca tgacgacggc gagagggacc gaggccatcc tgccgaccgc
     4921 acagatcatg gaccgcacct gttatgggcg acagcaggac tgggaggaca gtcccgaggg
     4981 ctggccgcag taccccacct acggctaacc gtccaggcgt ggtggcggct ggggtatgac
     5041 tcaactgcac acctggagat cgatatgacc gctacctaca ccttcgacgt cttctccagc
     5101 cttgatggct acggcgccgc gggcggcgac tggaccggct actggggtaa gcaaggcccc
     5161 gagctgctcg agcaccgcct gaccttgtac ggcaccgagc agcggatggt cttcggcgcc
     5221 aacacctacc ggctcttcgc ccagatgctg gcctcgagta ccgaggactc cgacgtacgc
     5281 gacccctggg tgactcggat gtggcgccag ccggcaacgg tggtgtcgac gtccctggaa
     5341 ggaccactcg attggccgga cgcgaccctc gtaagcggcg acgccgtcga catcgtcgcc
     5401 aggctcaagg aagagtccga cgtgtcgctg cgctcacacg gcagcctgtc gatgaaccgc
     5461 gcgctgatgg ctgcgggact ggtcgaccgc gtgcaggtca cgctcttccc tgtcatcacc
     5521 ggccagaccg ggctggaccc gatctttcag ggtgcggccg acttcgatct ggaactggtc
     5581 gagagtcgca cactcgatgg acacatccaa gagctcatct accgacccag cctccattga
     5641 ccctgtgcgg ggtcgagcgc atcccacgac cagtcccacc acgcgctcat cgccagacga
     5701 gtgcctttct gctactccgg catccggccg gaaggacgcc gcgtctgagt ggcgcgagga
     5761 actactggag agtcgtgcta cgggaacgtt ccggcggagt gctgcaagag gcgccgacca
     5821 gagcccgtcc cggcgtagtc gacgcagacc ttcggttgca ctctcccatc aacatggacg
     5881 cctcaacccg agcggccgga gcccgccagc aaggccaaac gtgacgcacg ctgagcaggc
     5941 accgacctaa acctcggcta ccggctctga catcgggact tggtgttggg cgtggcccac
     6001 cgggtccatc gatccagagc ttgtgtgggt tggcgaaggc atgacaccgc cgactggcgg
     6061 ctaagtgacg caccctcagc gtcagttctc gaagtgagga ctcagcggac agcttgttgg
     6121 acgagacatc caccgggtac accgtgtcct cgtcggatca agccggcaga cggatgctga
     6181 ggcgagctgg ttaccgacct caggctgcgc cgtgcagggc cccgcctatc tctgagatcg
     6241 acttccgcgc gaccccaagt cacgtaagca cgctaggctg cccggctcgg ccggtgttgt
     6301 ccggtcgtgc gctggtgcgc ctgaggaggc tctatggagc ggctgtcgtc gttcgtgctc
     6361 gctcatcgcc ggtgggtgtt cgcgttctgg gtcgtgatgt tcgtggccgg ggtcgcagcg
     6421 gccggggcgg ttccgaaccg gttgagctac gacttctcgt tgcccgggca gcagggctac
     6481 gagaccgagc agaagatcat cgcctcctac cagggtgcga acgcgcaggc tgcgtacgtc
     6541 ccggtcgtga cggtcccgga tggtcagacc gtcgcgggct cggccgcaga cgtcaaggcg
     6601 gtcttcgacg ccctcggcag gatccccacc ctccgggtcg tcgactactc ccagacccac
     6661 gacaaggggt tcatcaccag tgacggccga acgacgttcg gcgtcatcta cgaaccgcag
     6721 cccaacgggt tcgtcgaccc caaccagaag cagatcgagg acaccttcaa cgcgcagctc
     6781 gagcagcacg ggttcaccgg gggtctgacg ggctaccaac agctgtcgtc gggcggcggc
     6841 agcgacagcg ggccgagcgt gctggaggag actctgctcg gagccctcgg cgccctggcc
     6901 gtgctcgcgt tcgtcttcgc gtcgctcctg gcgttggtgc cgctcctgat cgccgcggtc
     6961 tcgatcctca cgacgttctt gatcgtgctc cttctcacca ccttcaccga cgtgagcttc
     7021 gtcgttcagt tcctcatcgc cctggtgggg ttgggcgtgg cgatcgacta ttcgctgctc
     7081 gtggtgtcgc gatggcgtga ggagatcgcg catgggaacg acaaccatga ggccgtgcgc
     7141 atctcggtgc gaacggccgg tcatgcggtg ttcgcgtcgg gcgtgaccgt cgccatcagc
     7201 ctgatcgcac tcgtcgtcat tccggtgccg ctgctgcaga gcatggggta cggcggcatg
     7261 ctcatcccct tgtgcagcgt cgcggtcgtc ctgacactgc tgcccgcgat gctcgctgcg
     7321 gtcggcccgc gcgtggacat cccgcacata cgcaaggagg ggaccgcatc gcggggatgg
     7381 acggcgtggg cccggatgat cgtgaggcgc ccgtggctgt tcggcggggc agcgatcatc
     7441 gtcctcgcca tcctcatcgc ccccctgcga cacatccagt tcgggcagtc cagcaccgcc
     7501 tcccagggga catcgagccc cgcgtaccag accttgcaga ccctgatcga cggcgacgtc
     7561 ggcaccggtg tgaccactcc gatcgtgttg ctggcggaca atgagaacga tgccgcggcc
     7621 atcgcctcgg ccgcaggcaa ggtggaaggg atcagggtgg cggcggcgta cgccccgggc
     7681 accgacgggc ggaccgccat cgacgtcatc ccggacgacg agacggtgaa ctcgagctcg
     7741 gtcgaagtcg tcgaccgggt cacagcagcc accgagtcgt taccgggata catcggcacg
     7801 gccgggctgg gcgccacgat cgtcgactac cggcacgcgg tctacgacaa cttcccgtat
     7861 gtcatcgggc tgatcgcgtt catcacgttc ctgctcctca tgcgcacctt ccgctcggtc
     7921 ctgctgccga tcaaggcggt ggtgctgaac ctgatcagcc tcggggccgt catgggactg
     7981 gcgacctggt tctggcagga gggcaacggc tccagcgcgg tgttcggcat cgaagccacg
     8041 ggtgcgatga cgttctggct gcctccgctg atcttcgcgt tcctgttcgg cctctcgatg
     8101 gactacgagg tgttcatcct gacccggatg cgtgaggagt atgaccggag tggctccacc
     8161 gaggcggcgg tggtacgcgg catcggacgg acggggcgac tggtcacctc cgcagcgctg
     8221 atcctgttct tcgcgttcgc agccctcgcc tctgcaccgg gcaccgacat caaggtcttc
     8281 gccacgtgcc tgggcgtcgg catcctcatc gacgccacga tcgtccgcgc cgtgctggtc
     8341 ccgtcgctgg tcgtgctctt cgggcggtgg aactggtggc tgcctgcctg gatcgcccgg
     8401 atcctgcgag tggagccttc ccccttgcac gagcacggcg aacccgggct cgtcgacgtc
     8461 gatctgtccg agccccacgt tcccagctcg gcgtcggact gacatcgggt gccgagtcca
     8521 cacgtgcctt cgccgtcgcc aaccgatcgc gacccaatcc cccggtggaa ggacgcgggc
     8581 ttgtagcggc agtgagacgc gtgtgccagg ggatgctgtc ttccgtgacc ggtcaggatc
     8641 gccacctgca tcggaccctc cctctgcgcg ttagtggtga agttcgccgt cgctagttcc
     8701 gcatcctccc gcgtggccac cctgggatgc gcggcgactg tacgcctcat gaccctgccg
     8761 accgctatcc gttgtgatgg gacaccaacc ccgacaagcg cgtcagcagg acgttcccgg
     8821 cgcccggcac gagacggaaa cgtgttccta tgcgctggcc ctgcgctgtc cagctgccga
     8881 gactgcgtct ggtagccggg gcacggcatg actcgcgctc tcgcgtctgt cccattcatg
     8941 acgacgttcc ccgagagggc tgggcttgcc cgatggtgtc cagttcggcg gccaattggg
     9001 tgcgccagcg cgtgaacatg gccccgtacc acgagtccgc gggaaccctc gcgagggctg
     9061 cggcggcgtc gctggagcgt ccgtgttgca gggcgagggc ggcctcgaag acttcgccgt
     9121 agccggtggc ttcggtggcc agaggccggg gtacaccgcg aaccgccgcc gtgatcccga
     9181 gccagtgctg tcgggcggcg cgatcgcccc gtcgtccgtg cagatatgcg atcgcg
//
LOCUS       DATBFZ010000102         6146 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_2685925, whole genome shotgun sequence.
ACCESSION   DATBFZ010000102 DATBFZ010000000
VERSION     DATBFZ010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 6146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6146
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2685925"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..713
                     /gene="rlmN"
                     /locus_tag="VH419_03050"
     CDS             <1..713
                     /gene="rlmN"
                     /locus_tag="VH419_03050"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0002935 - tRNA
                     (adenine(37)-C2)-methyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0070040 - rRNA
                     (adenine(2503)-C2-)-methyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEY7042624.1"
                     /translation="ATGQGGLQRNMSTAEIVEQILAGARSLARGDVPGGVARVSNVVF
                     MGMGEPMANYAAVMGSVRRMTDPDPDGLGMSARGITVSTVGLVPRMRQLAEEGIPVTL
                     ALSLHAPDDELRNDLVPINTRWSVAEAVEAAWNYARVTKRRVSIEYALIKDINDQAWR
                     ADLLGDVLRSYGDWGWVHVNLIPLNPTPGSKWTASRHEDEREFVRRLEAKGVPTTVRD
                     TRGREIDGACGQLAATQT"
     gene            710..1516
                     /locus_tag="VH419_03055"
     CDS             710..1516
                     /locus_tag="VH419_03055"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEY7042625.1"
                     /translation="MNPPDLRPPRLVVSDLDGTLLRSDGTFSERTVAAVADLQATGIG
                     WVVATARPPRWMHDLGPLVGDEGVAICSNGAFVYDVANRTVVAERTIPLEALTEIVSD
                     LKDAVPDISFAVEHRDGFGRETGYEDEYREADIRLVAAVPELFEPLPGKLLARSAAAD
                     HATFLATVATVVGDRAVVAFSGATGLAEISAAGVTKAAVLATWCEERGLEADDVWAFG
                     DMPNDLPMLSWAGTAFAVANAHPDVVAVADHTVASNDEDGVAQILETLLT"
     gene            complement(1847..2725)
                     /locus_tag="VH419_03060"
     CDS             complement(1847..2725)
                     /locus_tag="VH419_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008363272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIO1 family regulatory kinase/ATPase"
                     /protein_id="HEY7042626.1"
                     /translation="MHVITSPSTTGGSPPLSLSSSDADFIFTFTEYDDLADDQRWSTW
                     SSVEKGAHGPEPRPDWVVTDDGAIDTELGLLKTGKEADVFLLERAVPDDPDRSSLLAA
                     KRYRDSDHRQFHRDAGYVEGRRIRKSREARAAARGTAFGRSVQAGEWAIHEFAALSSL
                     WSAGVPVPYPVQIDGTEILMEFIDVDGNAAPRLAQVRPDRATVESYFEQIRDAMAILA
                     RRHLAHGDLSAYNILAQGDRVVIIDLPQVIDIVSNHNGVDFLLRDCRNICSWFRSRGL
                     DVDPDELLAELLAQAW"
     gene            2991..4877
                     /locus_tag="VH419_03065"
     CDS             2991..4877
                     /locus_tag="VH419_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020386056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA synthetase"
                     /protein_id="HEY7042627.1"
                     /translation="MGSYRETYDASVDEPEAFWAGCVDLVDWFAKPTQVLDRTRPPFY
                     RWFADGSLNTCYNALDRHVVKGRADQTALIYDSAVTGRVERFSYAQLLERVAAFAGGL
                     RGLGVGPGDRVVIYMPMIPEAVIAMLACARLGAIHSVVFGGFAPNELAVRVDDAEPKV
                     VVTASCGIEPGRVVEYKPLVDRALTLATHEPDAVVVKQREQAPAEMVDGRDVDFEVLM
                     AAGLADPAPCAEVTGSDPLYILYTSGTTGKPKGIVRDNAGHAVALAWSMANIYDVSAG
                     DIWWTASDVGWVVGHSYIVYAPLIVGATTVLFEGKPVGTPDAGTFWRVVEDHGVQGLF
                     TAPTAIRAIKKEDPDGVQIARYDVSSLRQLFLAGERLDPETYSWATKHLGVPVIDHWW
                     QTETGWPIVANLRGLEPMEIKPGSPTVPVPGYDVVVLDERGEAVPAGGEGAICIRLPL
                     PPGTLPTLWRDDERFVSSYLSAYDGYYLSGDGGYIDEDGYVYVMGRTDDVINVAGHRL
                     STGSMEAVLAAHPDVAECAVIGAADELKGQLPRGFVVLKAGHEVDESRLAAELITAVR
                     EEIGPVAAFKDVRVVPALPKTRSGKILRKTMRQIADGGEPAVPSTIEDASVLDTIRPA
                     LRNS"
     gene            4937..5932
                     /gene="dhaK"
                     /locus_tag="VH419_03070"
     CDS             4937..5932
                     /gene="dhaK"
                     /locus_tag="VH419_03070"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020657320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HEY7042628.1"
                     /translation="MKKLINDPADVVKDALLGIEAAHGDRVRVDHELKVIYRKDAPRQ
                     GKVGLISGGGSGHEPMHGGYVGLGMLDAACAGEVFTSPVPDQMMAATKEVDGGAGVLH
                     IVKNYTGDVMNFEMAAEMAEGVEVKSVVTDDDVAVQDSLYTAGRRGVGVTVLLEKIVG
                     AAAEEGRPLADVADLATKVNDSGRSMGMALTSCTVPAAGKPTFELGEDEMEVGIGIHG
                     EPGRSRVSLASAREIAAMLVDPIVGDLPYGQGDSVIAFVNGMGGTPLIELYVAFDSVS
                     RILGGQGISIARSLVGNYITSLEMAGFSVTLLRLDDALIRWWDAPVHAPALRWGI"
BASE COUNT         1001 a         2022 c         2170 g          953 t
ORIGIN      
        1 gcgcaaccgg acaaggtggg ttgcagcgca acatgagcac cgccgagatc gtcgagcaga
       61 tcctcgccgg agcccgttcg ctggcccgcg gcgacgtgcc gggtggcgtt gccagggtct
      121 ccaacgtcgt gttcatgggg atgggcgaac cgatggccaa ctacgccgcg gtcatgggtt
      181 ccgtgcgccg gatgaccgat cccgatcccg acggcctcgg aatgtcggcg cggggcatca
      241 ccgtgtccac cgtcggtctc gtgccccgga tgcggcagct tgcggaggag gggatcccgg
      301 tcacgctcgc gctgtcgctg cacgcgccgg acgacgagct gcgcaacgac ctggtgccca
      361 tcaacacgcg ctggagcgtg gccgaggcag tcgaggcggc ctggaactac gcacgcgtca
      421 ccaagcgccg ggtctcgatc gagtacgccc tgatcaagga catcaacgat caagcgtggc
      481 gggccgacct gctgggagac gtcctccggt cgtacggcga ctggggctgg gtgcacgtca
      541 acctcatccc gctcaacccg actccaggct cgaagtggac ggcctcacgg cacgaggacg
      601 aacgcgagtt cgtccgccgc ctcgaggcca agggagtccc cacgacggtg cgcgacacac
      661 gtggccgcga gatcgacggc gcctgcggcc agctcgccgc cacgcagacg tgaacccgcc
      721 ggacctgagg cctcctcggc tggtcgtctc cgacctggac gggacgctgc tgcgaagcga
      781 cgggacgttc agcgagcgga ccgtcgccgc ggtcgcagac ctgcaggcga ccggcatcgg
      841 ctgggtcgtc gcgaccgcac ggccaccacg atggatgcat gacctcggtc cgctggtcgg
      901 tgacgagggt gtcgcgatct gctccaacgg tgcgtttgtg tacgacgtcg cgaaccggac
      961 cgtcgtcgcc gaacggacga tcccgctcga ggcgctgacc gagatcgtca gcgacctcaa
     1021 agacgcggtg ccggacatct cgttcgcggt ggagcatcgg gacggcttcg gccgcgagac
     1081 cggctacgag gacgagtacc gagaggccga cattcgactg gtggccgcgg tcccggagct
     1141 cttcgaaccg ctcccgggca agctgctggc acgatctgcg gccgccgacc acgcgacgtt
     1201 cctcgccacc gtggcgacgg tcgtgggtga ccgggcggtg gtggccttct ccggtgccac
     1261 gggtcttgcc gagatcagcg ccgccggcgt gacgaaggcg gcggtgctcg ccacctggtg
     1321 cgaggagcga ggactcgagg cagacgacgt ctgggcattc ggagatatgc ccaacgacct
     1381 cccgatgctc agctgggccg gcaccgcgtt cgccgtggcc aacgcgcacc cggacgtcgt
     1441 cgcggtcgcc gaccacacgg tggcgagcaa cgacgaggac ggcgtcgccc agatcctcga
     1501 aaccctcctc acctgacccc acctccatcg ttgagtgccg actttctggg tgttgagtgc
     1561 cgactttccg ggtgttgagc gccggctttc ggggtgttga gtgcgggctc acgggcaggt
     1621 gagtgcgggc tcacgggcag gtgagtgctg gacatccgga atcaactcga gcctccgaga
     1681 ctccgtaccg ccgaaaggcc gcactcaacg gtccgcaacc cagcagtcaa cggccaggaa
     1741 cccagcactc gatggccagg aacccagcac tcgacggcaa gcaacccagc actcgacggc
     1801 cagcaagcca gcattcaacg acgagggggg tggcggcgta ttcaggtcac caggcttggg
     1861 cgaggagctc ggcgaggagc tcgtcggggt cgacgtcgag accgcgcgac cggaaccagc
     1921 tgcagatgtt gcggcagtcc cgcagcagga agtcgacgcc gttgtggttg ctcacgatgt
     1981 cgatcacctg tggcaggtcg atgatcacca cgcggtcgcc ctgggcgagg atgttgtacg
     2041 ccgagaggtc cccgtgcgcg aggtgtcgac gggcgaggat cgccatcgcg tcgcggatct
     2101 gctcgaagta cgactcaacg gtcgcccggt cgggccggac ctgcgcgagt cgcggcgcgg
     2161 cgttgccgtc gacgtcgatg aactccatca ggatctcggt gccgtcgatc tggacgggat
     2221 acgggaccgg tacgccggcc gaccacagcg acgagagcgc ggcgaactcg tggatcgccc
     2281 actcgcccgc ctggacgctg cgaccgaacg cggtgcctct ggccgcggcg cgcgcctcgc
     2341 gactcttccg gatgcgccgc ccctcgacgt agccggcgtc ccggtggaac tgccggtggt
     2401 cggagtcgcg gtagcgcttc gcggccagca gcgaggaccg gtccgggtca tcgggaacgg
     2461 cgcgttcgag caggaagacg tcggcctcct tgccggtctt caggagtccg agctcggtgt
     2521 cgatggcgcc gtcgtcggtg acgacccagt ccggacgtgg ctcagggcca tgggcgccct
     2581 tctcgaccga tgaccaggtc gaccaccgct ggtcgtcggc gaggtcgtcg tactccgtga
     2641 aggtgaagat gaaatccgca tcagacgaac tcaggctgag cggaggggag ccgcccgtgg
     2701 tggacggcga tgtgatgacg tgcatggtgg tggttgctcc tcgagcgtgt cgaggtcacc
     2761 gaccagcggt cagccgcgaa gggacctcga ggatgaaggg acgcagaagt gctgccccag
     2821 gaccggggtg gacatgtcga cgcctccttc gctcggcgac gggcaccagc ccgtcgtacg
     2881 gatgaccgcc gcagagcctc gcacacggcc gccggagcgt gcaacaggtt tttctgcggg
     2941 ccgggtttgg caggatgggg tcgatctcga ccaaccggga ggtcaggccg atgggcagct
     3001 accgcgagac gtacgacgcc agcgtcgacg agccggaggc gttctgggcc ggatgtgtcg
     3061 acctggtcga ctggttcgcc aaaccgaccc aggtgctcga ccggacgcgg ccaccgttct
     3121 atcgctggtt cgccgacggc agcctgaaca cctgctacaa cgcgttggac cgacacgtgg
     3181 tgaagggtcg cgccgaccag accgcgctca tctacgacag tgccgtgacc ggcagggtcg
     3241 aacgcttctc ctatgcgcag ctgctcgaac gggtcgcggc attcgcgggc ggcctgcgcg
     3301 gtctcggcgt agggccgggt gaccgggtcg tcatctacat gccgatgatc ccggaagcgg
     3361 tcatcgcgat gctggcgtgc gctcgactgg gggcgatcca ttcggtggtg ttcggcgggt
     3421 tcgcgccgaa cgagctcgcg gtgcgcgtcg acgacgccga gccgaaggtc gtcgtcaccg
     3481 cgtcctgcgg gatcgagccc ggccgggtcg tcgagtacaa gccgctggtc gatcgcgcgc
     3541 tgaccctcgc gacgcacgag ccggacgcgg tcgtggtcaa acagcgtgag caggcgccgg
     3601 cggagatggt cgatggacgc gacgtcgact tcgaggtcct gatggcggct gggctggccg
     3661 atcccgcccc gtgcgccgag gtgaccggct ccgacccgtt gtacatcctc tacacatcag
     3721 gtacgaccgg gaagccgaag ggcatcgttc gtgacaacgc cggtcatgcg gtcgccctgg
     3781 cgtggtcgat ggccaacatc tacgacgtgt cagccggtga catctggtgg acggcgtcgg
     3841 acgtcggatg ggtcgtcgga cactcctaca tcgtgtacgc cccgctgatc gtcggcgcga
     3901 cgacggtgct gttcgagggg aagccggtcg gaaccccgga cgcaggcacc ttctggcgtg
     3961 tggtcgagga tcacggtgtg cagggactgt tcacggctcc caccgcgata cgcgcgatca
     4021 agaaggagga ccccgacggt gtgcagatcg cgcggtacga cgtgtcgagc ctccgccagc
     4081 tgttcctcgc cggggagcgg ctcgatccgg agacgtactc ctgggcgacg aagcatctcg
     4141 gtgtccccgt gatcgaccac tggtggcaga ccgagacagg gtggccgatc gtcgccaacc
     4201 tgcggggtct cgagccgatg gagatcaagc cagggtcgcc gacggtgccc gtccccgggt
     4261 acgacgtcgt ggtcctcgac gaacgaggcg aggcggtccc cgccggggga gagggcgcca
     4321 tctgcatccg actgccgctg ccccccggga cgctcccgac actgtggcga gacgacgaga
     4381 ggttcgtgtc gtcgtacctg agcgcctacg acggctacta cctctccggc gacggcggat
     4441 acatcgacga ggacggctac gtctacgtga tgggccgtac cgacgacgtc atcaacgtcg
     4501 cgggacaccg gctgtcgacc gggtcgatgg aggcggtcct ggccgcccac cccgacgtcg
     4561 cggagtgcgc ggtgatcggc gcagccgacg agctgaaggg ccagctgcca cgcggcttcg
     4621 tcgtgttgaa ggcaggccat gaggtcgacg agtcgcggct ggcggcagag ctgatcaccg
     4681 ctgtgcgcga ggagatcggt ccggtggcgg cgttcaagga cgtccgagtg gtcccggcgc
     4741 tgccgaagac ccggtcgggc aagatcctcc gcaagacgat gcggcagatc gccgacggtg
     4801 gcgagccggc ggttccgtcc acgatcgagg acgcctccgt cctggacacg atccgtccgg
     4861 ccctgcgcaa ctcgtgacgt ccggcggctg acaagggcta ttgcccgcgt ctcgtcgttt
     4921 ctgctcagat gaggtcatga agaagctcat caacgaccct gccgacgtcg tcaaggacgc
     4981 actgctcggc atcgaagcag cccacggtga ccgagttcgt gtcgaccacg agctcaaggt
     5041 catctaccgc aaggacgctc cccggcaggg caaggtgggc ctcatctccg gcggcggttc
     5101 cggccacgag cccatgcacg gcgggtatgt cggactcggc atgctcgacg cggcatgtgc
     5161 cggagaggtc ttcacctcac ctgtgccgga ccagatgatg gcggcgacca aggaggtcga
     5221 cggcggcgcc ggcgtcctcc acatcgtcaa gaactacacc ggcgacgtga tgaacttcga
     5281 gatggccgcc gagatggccg aaggcgtcga ggtcaagtcc gtcgtgaccg acgacgacgt
     5341 cgccgtacag gacagtctct acacggccgg ccgtcgtggc gtcggcgtca ccgtgctgct
     5401 ggagaagatc gtcggcgccg cagccgagga gggccgccca ctggccgacg tggccgacct
     5461 cgcgaccaag gtcaacgaca gcggacgcag catggggatg gcgctcacgt cgtgcaccgt
     5521 tccggccgcc ggcaagccga cgttcgaact cggcgaggac gagatggagg tcggcatcgg
     5581 catccacggc gagcccggac gttcccgcgt gtcgctcgcc tccgcccggg agatcgccgc
     5641 gatgctcgtc gacccgatcg taggcgacct gccgtacggc cagggcgact cggtcatcgc
     5701 gttcgtcaat ggcatgggcg gaacgccgct catcgagctc tatgtcgcgt tcgacagcgt
     5761 ctcgcggatt ctcgggggtc aggggatctc gatcgcccgt tcgctcgtgg gcaactacat
     5821 caccagcctc gagatggcgg gcttttccgt gacgctcctg cggctggacg acgccttgat
     5881 ccgctggtgg gacgcccccg tccacgcgcc tgcactgcgg tgggggatct gactcgtcgg
     5941 tctcgccggc gccgcgcccg atgtcctcgg gcgcggtgac cggcaggctg tcggtgcgac
     6001 ccacgatggc gatgacctct tctagactcg cgctcgatgc gccgagccaa ggtaggactc
     6061 cgatgatcga cacgatcgat gcatggctgc gcctcgccgc ggccaggatc catgacgctg
     6121 ctccgacctt gaccgccctc gaccag
//
LOCUS       DATBFZ010000103         2870 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_3999335, whole genome shotgun sequence.
ACCESSION   DATBFZ010000103 DATBFZ010000000
VERSION     DATBFZ010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2870
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3999335"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="VH419_03075"
     CDS             complement(<1..292)
                     /locus_tag="VH419_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012923250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY7042629.1"
                     /translation="MSTSQSPHIVIAGGGYVGMYTALRLQKKLRRGEARVTVIDPRST
                     MTYQPFLPEAAAGSLEPRHVVVSLRRTLKRTNVITARVESIDTAKKTVRVVPF"
     gene            complement(429..1679)
                     /locus_tag="VH419_03080"
     CDS             complement(429..1679)
                     /locus_tag="VH419_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007078868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEY7042630.1"
                     /translation="MTETSLARRLGVGDAVVVGLSAMVGAGVFAAFAPAAEAAGSLLL
                     VGLGLAAVVAYCNATSSAQLAAQYPSSGGTYVYGRERLGEWWGFLAGWGFVVGKTASC
                     AAMALTFAAYAVDGPEWVQRLVALVAVVALTAVNYRGITKTSGLARVLLAVTLVALVV
                     GVAGIVIDLDGTSHLSDGEAWTNGSAYGVLQSAGLLFFAFAGYARIATLGEEVRDPET
                     TIPRAIPLALGIAVVLYAVVGVVLLVALGPDRLAASVAPLDTAASDVAAWLGWLVRVG
                     AVAGALGALLALVAGVGRTTLAMARGGDLPRFLDRVHPRHQVPHRAEVAVGAVVGLLV
                     LTTDLRGVIGFSSFGVLLYYAVANASALTQTPGHRRWPKGLQVLGLVGCLVLVAALPW
                     QAVVAGVSVFVVGVVARLAGSLTR"
     gene            complement(1695..2231)
                     /locus_tag="VH419_03085"
     CDS             complement(1695..2231)
                     /locus_tag="VH419_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042631.1"
                     /translation="MSTSLKNPMEGMALPFREPLESRPLSAPVIPEPLRRGEQGGPPC
                     GVCDGSSTSAVWADDLWTLHPPVGGSMPGSLWMASREHADSFMDLSPEAQASFGGVVG
                     RVEKALLSVGDFARVHLYRWGDGGAHFHVWFMPRPIGMLEATGMMLPLWEDALPNVED
                     EELRQFAEKVAAVLESDG"
     gene            complement(2321..>2870)
                     /locus_tag="VH419_03090"
     CDS             complement(2321..>2870)
                     /locus_tag="VH419_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEY7042632.1"
                     /translation="DAQPAVLIVGLAPAANGGNRTGRIFTGDRSGDWLFASLHRVGLA
                     RQATSVHAGDGQELIGTRMMAAVRCAPPANKPTPIERDTCEPWLDAELALALPSARAL
                     VCLGSFAWDVALRALARAGLEVPRPRPRFGHHARVEIGAVTVLGCYHPSQQNTFTGKL
                     TETMLDAVLADAARLGGLASSG"
BASE COUNT          493 a         1049 c          960 g          368 t
ORIGIN      
        1 cgaacgggac gacccgcacc gtcttcttgg cggtgtcgat cgactcgacc cgggcggtga
       61 tgacgttggt cctcttcagc gtccggcgca acgagacgac gacgtgacgc ggctcgagcg
      121 atccggctgc ggcctcgggc aggaacggct ggtaggtcat cgtggagcgc gggtcgatca
      181 ccgtgacccg ggcctcgccc cgacgcagct tcttctgcag gcgtagagcg gtgtacatcc
      241 cgacgtatcc gccgccggcg atgacgatgt gcggcgactg gctggtgctc atcggaggtc
      301 ccttcctcgt cctcgagcgt atgccgaaca cctccagact aacgctcgtg aaaattttca
      361 caagagtctg tcgacgtgat ctcgcccatg acgggcagct ctggtcagcg ggtcagcggg
      421 tcagcgggtc agcgggtcag cgagccggcc aagcgggcca cgacgccgac cacgaagacc
      481 gacaccccgg cgacgacagc ctgccagggc agcgccgcga ccagcacgag acatccgacc
      541 agcccgagca cctgcaaccc cttcggccag cggcgatgcc caggggtctg ggtcagggcg
      601 ctggcgttgg cgacggcgta gtagagcagc acgccgaacg acgagaaccc gatcacgccg
      661 cgcaggtcgg tggtcagcac gagcagcccg acgaccgctc cgacggcgac ctcggcgcga
      721 tgcggcacct gatgacgcgg gtgcacccgg tcgaggaagc ggggcaggtc accgccgcgc
      781 gccatcgcca gcgtcgtccg accgaccccg gccacgagtg ccagcaacgc accgagcgct
      841 cccgcgacag caccgacccg gaccaaccag cccagccagg ccgcgacgtc ggaggcggcg
      901 gtgtccaacg gcgcgacact ggcggccagc cggtcgggcc cgagcgcgac gagcagcacg
      961 accccgacga cggcgtacag cacgacggcg atgccgaggg ccaacgggat cgcgcgcggg
     1021 atcgtcgtct cggggtcgcg cacctcctcc cccagcgtcg cgatccgcgc gtacccggcg
     1081 aaggcgaaga acaacaaacc ggcggactgc aggacgccgt aggcgctgcc gttcgtccag
     1141 gcttcgccat cgctcaggtg cgacgtgccg tcgagatcga tcacgatgcc ggccacgccg
     1201 acgaccaggg ccacgagcgt gaccgcgagc agcacgcggg cgagaccgga cgtcttggtg
     1261 atcccgcggt agttcacggc cgtgagtgca accacggcga ccagcgcgac gagacgctgc
     1321 acccattcag gcccgtcgac ggcgtacgcc gcgaacgtca gcgccatcgc tgcgcaggag
     1381 gcggtcttgc cgacgacgaa tccccacccg gcgaggaacc cccaccactc gccgagccgc
     1441 tctctgccgt agacgtaggt gccaccggac gacgggtact gggcagccag ctgggccgac
     1501 gacgtggcgt tgcagtaggc caccaccgcg gccagcccca gcccgacgag caacaggctc
     1561 ccggcagcct cggccgccgg tgcgaacgcg gcgaagacgc cggctccgac catcgccgac
     1621 agcccgacga cgaccgcgtc accgacgccg agacgtcgag ccagcgaggt ctcggtcatc
     1681 gagcgctcac gacatcagcc atcggactcc agcacggccg cgaccttttc tgcgaactgc
     1741 cggagctctt cgtcctcgac gttcggcaag gcgtcctccc acagcggcag catcatcccg
     1801 gtcgcctcga gcatgccaat cgggcgcggc atgaaccaga cgtggaagtg cgctccgcca
     1861 tcaccccaac ggtagaggtg gacgcgggcg aagtcgccga ccgacaacaa ggctttctcc
     1921 acccggccca cgaccccgcc gaagctcgcc tgtgcctccg gcgacaggtc catgaacgag
     1981 tccgcgtgct cccgggacgc catccacaac gatcccggca tgctgccgcc gaccggtggg
     2041 tgcagcgtcc agaggtcgtc cgcccataca gcagacgtgc tggatccgtc gcagacaccg
     2101 catggaggac cgccctgctc accacggcgc agtggctcgg ggatcaccgg agcggacagg
     2161 ggccgcgact cgagtggttc gcggaagggc agcgccatcc cctccatcgg gttcttcaag
     2221 ctggtgctca tcgcgtgctc cttcggcgta cgagccctgg ctgggcttcc tcgccctcag
     2281 tctcggagca acgcacatcg gcgcacttga tcgagtttgc tcatccgctg gacgccaagc
     2341 caccgagtcg ggccgcatcc gccaggaccg cgtcgagcat ggtctcggtg agcttccccg
     2401 tgaacgtgtt ctgctggctc gggtgatagc agccgagcac cgtcaccgcc ccgatctcga
     2461 cccgcgcatg gtgaccgaag cgaggtcgcg gtcgcggcac ttcgaggccg gctcgggcga
     2521 gagcgcgcag cgcgacgtcc caggcgaacg agccgaggca caccagcgcc cgcgccgacg
     2581 gcagcgccag ggcgagctcg gcgtccagcc agggctcaca ggtgtcacgt tcgatcggag
     2641 tcggcttgtt cgccggcgga gcgcagcgga ccgcggccat catccgggtg ccgatcagct
     2701 cctgtccgtc ccccgcgtgc acgctggtcg cctggcgggc caggccgacc cggtgcaacg
     2761 acgcgaacag ccagtctccg gatcggtcgc cggtgaagat ccgaccggtc cggttgcctc
     2821 cgttcgcagc aggcgccaac ccgacgatca gcacggccgg ctgggcgtcg
//
LOCUS       DATBFZ010000104         4250 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_1373923, whole genome shotgun sequence.
ACCESSION   DATBFZ010000104 DATBFZ010000000
VERSION     DATBFZ010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 4250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4250
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1373923"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            82..1131
                     /locus_tag="VH419_03095"
     CDS             82..1131
                     /locus_tag="VH419_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007077551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY7042633.1"
                     /translation="MPADNETTPTTEATEAPESAASGTATGTMTEAAPVTLDVEAARK
                     ALEGVEHEPGAPKPDPILVADNVVRRFGGLTAVDVDHVEVQRGVITALIGPNGAGKTT
                     FFNLLTGFDKPDEGDWSFEGRSVKGMSAYQVARRGMVRTFQLTKVLARLSVIENMRLG
                     ATGQSGESLWQAMFRPFWGGQEKANTQRADKLLERFKLDTKRGDFAGSLSGGQRKLLE
                     MARALMVEPTLVMLDEPMAGVNPALKQSLLGHVKSLRDEGMTVLFVEHDMDMVRDISD
                     WVIVMAQGRIISEGPPEAVMADQRVIDAYLGAHHDAALTFEAEEEILAAAQEELEAEA
                     QPEEPTVDAKEAERA"
     gene            1124..1948
                     /locus_tag="VH419_03100"
     CDS             1124..1948
                     /locus_tag="VH419_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007077550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY7042634.1"
                     /translation="MPDPNGTPPVSEETAGVASTVTDSPGREAHVAAAEGSVLRADDL
                     IAGYLPGVNILNGADLYCQDGELVGIIGPNGAGKSTLLKALFGLVKVTSGKVTLKGED
                     VTNERADVLVTSGIGFVPQTNNVFPSLTIAENLEMGSYQKPKNFRKRFDFVAELFPAL
                     RDRRAQRAGSLSGGERQMVAMGRALMMEPSVLLLDEPSAGLSPVMQDEVFVQTRRINR
                     AGVSVVMVEQNASRCLQICDRAYVLDQGRNAYTGTGRDLANDPKVIELYLGTLATT"
     gene            complement(2136..3425)
                     /locus_tag="VH419_03105"
     CDS             complement(2136..3425)
                     /locus_tag="VH419_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007077549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY7042635.1"
                     /translation="MRFTRTARLATVSAAAALVLASCGGSDDNGGGGGESTTTSNESP
                     SAAKGDGTLVVGSLLPTTGDLAFLGPPEIAGVDTAIEDINAAGGVLGQQVTHVEQDSG
                     DGTPNIAPASVDKLLNEKADVIIGAASSSVSLSVIDKIVNAGVVQFSPANTSTAFDTY
                     DDKGLYFRTAPSDVLQGAVMANMVVDDGFKNVAILARQDSYGEALADQVAKGIEEGGS
                     KVATKTLYSADASNFTAEVNKVAATNPDAIVLIAFNETTKIIPTLIGKGIGPKDVQIY
                     FVDGNTADYSKDFDPGTLAGNGYAVKATYPGAELTADFKARLLKTDPSLKDFTYGPES
                     YDATIMSALAATAAKDDAGEAIGSKLVEISEGGTKCTEYKECVDLLNQGEDIDYDGVS
                     GPVDLNDTGSPSKATIGIFQYNSKNTYSNLDYVTGVI"
     gene            complement(3553..4137)
                     /locus_tag="VH419_03110"
     CDS             complement(3553..4137)
                     /locus_tag="VH419_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009079762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY7042636.1"
                     /translation="MPTPTVVIAEDEALIRLDLAEMLAEEGYDVVGQAGDGERAVELV
                     EALRPDLVVLDVKMPKLDGISAAARIAEQRIAPVVVLTAFSQRELVERARDAGAMAYV
                     VKPFGKHDLVPAIEMARSRFAELRQLETEVADLSERLETRKAVDRAKGMIQEALGVSE
                     PEAFRWIQKTAMDLRLSMRDVAQGVVTHGLEVTK"
BASE COUNT          700 a         1383 c         1441 g          726 t
ORIGIN      
        1 tcttcctggg gctcgcgctc atgctgctca tgatcttccg cccacaaggc atcctgggtg
       61 accgaaggga gctggcgctc gatgcccgct gacaacgaga cgacgccgac gacagaggcg
      121 accgaggcgc cggagtccgc ggccagcggg acggcgacgg gcacgatgac cgaggcagct
      181 ccggtgacgc tcgacgtcga ggccgcgcgc aaggctctcg agggtgtgga gcacgagccg
      241 ggtgcgccga agccggaccc gatcctggtg gccgacaacg tcgttcgcag gttcggcggc
      301 ctgaccgccg tcgacgtcga ccacgtcgag gtccagcgag gcgtcatcac ggcgctgatc
      361 ggcccgaacg gtgccggcaa gacgacgttc ttcaatctgc tcaccgggtt cgacaagccg
      421 gacgagggcg actggtcgtt cgagggtcgc agtgtgaaag gcatgtcggc gtaccaggtt
      481 gcgcgccgcg ggatggtccg gaccttccag ctgaccaagg tgctggcaag gctcagcgtg
      541 atcgagaaca tgcggcttgg tgccaccgga cagtccggcg agagcctctg gcaggcgatg
      601 ttccggccgt tctggggcgg ccaggagaag gcgaacaccc aacgcgccga caagctgctc
      661 gagcgattca agctcgacac gaagcgcggt gacttcgccg gctcgttgtc gggcggtcaa
      721 cgcaagttgc tggagatggc tcgggcattg atggtcgagc cgacgctggt gatgctcgac
      781 gagccgatgg ccggagtcaa cccggcgctg aagcagtcac tgctcggcca cgtgaagtcg
      841 ctgcgcgacg agggcatgac cgtgttgttc gtcgaacacg acatggacat ggtccgcgac
      901 atctccgact gggtgatcgt gatggcgcag ggccggatca tctccgaggg tccgcccgag
      961 gcggtgatgg cggaccagcg cgtgatcgac gcctacctgg gcgcgcacca cgacgcggca
     1021 ctgacattcg aggccgagga ggagatcctc gcggcggccc aggaggagct cgaggccgag
     1081 gcccagcccg aggagccgac ggttgacgcg aaggaggccg aacgtgcctg acccgaacgg
     1141 caccccaccg gtctccgagg agacggcggg ggtcgccagc acggtgaccg actcgcccgg
     1201 gcgagaggcg cacgtcgcgg ccgcggaggg atcagtgctc cgggctgacg acctgatcgc
     1261 cggctatctt cccggcgtca acatcctcaa cggtgcggac ctgtactgcc aggacggcga
     1321 gctggtcggc atcatcggcc cgaacggcgc aggtaagtcg acgctgctca aggcgttgtt
     1381 cggcctggtc aaggtgacct cgggcaaggt gacactcaaa ggcgaggacg tgaccaacga
     1441 gcgcgcggac gtgctggtga catccggcat cggtttcgtg ccgcagacca acaacgtctt
     1501 cccctccctg accatcgccg agaacctcga gatgggcagc taccagaagc cgaagaactt
     1561 ccggaagcgt ttcgacttcg ttgccgagct gtttccggcg ctgcgtgacc ggcgagccca
     1621 gcgcgccggc tcactgtccg gtggtgagcg acagatggtc gccatgggcc gcgcactgat
     1681 gatggagcca tcggtgctgc tgctcgacga gccgtccgcc ggcctgtcgc cggtcatgca
     1741 ggacgaggtc ttcgtgcaga cccgccgtat caaccgcgcg ggcgtctcgg tcgtgatggt
     1801 cgagcagaac gccagccgat gtctccagat ctgtgaccgt gcctacgtcc tggaccaggg
     1861 acgcaacgcc tacaccggca cgggcagaga cctggccaac gaccccaagg tcatcgagct
     1921 ctacctcggc acgctcgcaa cgacctgacg tgcggccggg cccactgtgc gagggcgaac
     1981 ctcggccgtg gtcggtgatc tcgacaagct cgatcagcgg aacaccgttg atctcgacaa
     2041 gctcgatcag cgacacgccc gatcagcgga acaacgaagg gccccgacaa cctggtcggg
     2101 gcccttcggc tactccctcg tacgagtccg ggtgatcaga tcacaccggt gacgtagtcg
     2161 aggttgctgt aggtgttctt gctgttgtac tggaagatgc cgatcgtcgc cttcgacggg
     2221 ctgcccgtgt cgttcaggtc gaccggaccc gagacgccgt cgtagtcgat gtcctcgccc
     2281 tggttgagca ggtcgacgca ctccttgtac tcggtgcact tcgtgccgcc ctcggagatc
     2341 tcgaccagct tcgacccgat cgcctcaccg gcgtcgtcct tggccgcggt cgcggcgaga
     2401 gccgacatga tcgtcgcgtc gtacgactcc ggaccgtagg tgaagtcctt cagcgacggg
     2461 tcggtcttga gcaggcgggc cttgaagtcc gcggtcagct cggcaccggg gtacgtcgcc
     2521 ttgaccgcgt aaccgttgcc ggccagcgtg cccgggtcga agtccttcga gtagtcggcc
     2581 gtgttgccgt cgacgaagta gatctggacg tccttcgggc cgatgccctt gccgatgagc
     2641 gtcgggatga tcttcgtggt ctcgttgaac gcgatcagca cgatggcgtc tgggttggtc
     2701 gccgcgacct tgttgacctc ggcggtgaag ttcgacgcgt cggccgagta gagggtcttg
     2761 gtggcgacct tcgagccgcc ctcttcgatg cccttcgcga cctggtcggc gagcgcctca
     2821 ccgtaggagt cctgacgggc caggatcgcg acgttcttga agccgtcgtc gacgaccatg
     2881 ttggccatca cggcgccctg caggacgtcg gacggggcgg tccggaagta cagccccttg
     2941 tcgtcgtagg tgtcgaacgc ggtcgacgtg ttggcgggag agaactgcac cacgccggcg
     3001 ttgacgatct tgtcgatgac gctcagcgag acgcttgacg acgccgcacc gatgatgacg
     3061 tcggccttct cgttgagcag cttgtcgacc gacgcaggtg cgatgttcgg cgtaccgtca
     3121 ccggagtcct gctcgacgtg ggtgacctgc tggccgagca ctccaccggc ggcgttgatg
     3181 tcctcgatcg ccgtgtcgac accggcgatc tcgggcgggc cgaggaacgc aaggtcaccg
     3241 gtggtcggga gcaaggagcc gacgacgagg gtgccgtcgc ccttggccgc ggacgggctc
     3301 tcgttcgacg tggtggtcga ctcgccgccg ccgccaccgt tgtcgtcgct gcccccgcag
     3361 gatgcaagga caagcgcggc cgcagcggac accgttgcca gccgcgctgt tcgcgtgaaa
     3421 cgcatggaac ctccgtggtt cgaggggatc gttccgaggc gggggtcttc cagcccggat
     3481 ttgatggaga gcaacctagc ctgttcgtgc ccgagaatca cattttcgtc gttcgcgagc
     3541 cgatcgtcgt tgtcatttcg tgacctcgag gccgtgggtg accacaccct gcgcgacatc
     3601 tcgcatcgaa agccgcaagt ccatcgccgt tttctggatc cagcggaacg cctccggctc
     3661 gctcacaccc agtgcctcct ggatcatccc cttcgcccga tccaccgcct tgcgtgtctc
     3721 cagccgctca ctgaggtctg caacctcggt ctccagctgg cgcagctcgg cgaaccgtga
     3781 ccgcgccatc tcgatggccg gcacgaggtc atgcttgccg aacggcttga ccacgtatgc
     3841 catcgcgccg gcgtcacgtg cccgctcgac cagctcgcgc tggctgaacg cggtgaggac
     3901 gacgacgggg gcgatccgtt gctcggcgat ccgggcggcg gcggagatgc cgtcgagctt
     3961 cggcatcttc acgtcgagga cgacgaggtc ggggcgcaac gcttcgacga gctcgacggc
     4021 ccgttcaccg tcgccggcct ggccgacgac gtcgtacccc tcctcagcga gcatctcggc
     4081 caggtccagc cggatcagcg cctcgtcctc cgcgatcacg accgtcggcg taggcatcgc
     4141 agcggccggt tccacgccgg atccagacac gagcaaaggg tatcgtccgg gttggccgcc
     4201 gtacttttcg gcggctaacg taggagcctc gccctggtat tccaaccggc
//
LOCUS       DATBFZ010000105         7333 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_717995, whole genome shotgun sequence.
ACCESSION   DATBFZ010000105 DATBFZ010000000
VERSION     DATBFZ010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 7333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7333
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_717995"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2080
                     /locus_tag="VH419_03115"
     CDS             <1..2080
                     /locus_tag="VH419_03115"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEY7042637.1"
                     /translation="GPGGAVPIPQSVMPLVAGRISALPATARKALVAAAVLVDPSLPA
                     IRSLTNNRGRLAGLGSAEVSGVVEIKDGRVRFTHPLLGSAAYASASEERRRRLHLKAA
                     ALAIDPEERAQHLALTAKGPSAAIAAALDVAAEWAESRGAPDRAAELWEAAAGLTPGT
                     RTEGRWRRAVRAAECYFQSGDNASARPLLEGVIAVAQAGPLRADALRALSMVQRRDGL
                     REAAEGSLRQASAEAGNATVTRARIELSLAYAVAWRRGLALGLRHAETTQRLLDEAAE
                     PGLAGAATALVFHLEYSMGREVRWDRLSDALALEATTLERGHTYLPVDERPSYFEAWA
                     LREADEQDRARERFEELRRTCADRGEEGSLAFLLHLMAELETSAGNFSLAARHADEAT
                     EVMVRSGLRQMESVSLYVRAHLEARQGKVAAARTHATRALEIATRAGSVPSIHDAAGV
                     LGFLALSIGDHLAADRLLTPAITMMVSLGVGDPAALRSVPDEIEALVTLGELGRAEAL
                     LDPFEQQASFMGRRWAAAAASRCRALVCSGHGDLAAAVGAADRAVDGHRELPYPFELG
                     RSLLVQGVVHRRRKEKAVAAEAFREAVEIFDRLGTPLWADKARAEIRRVGLRPRAPLE
                     LTATEARVAELAASGLTNREVADALFVSPKTVESNLAKVYRKLGIRSRAELGATIAAR
                     ERVAAKSRSG"
     gene            2323..3090
                     /locus_tag="VH419_03120"
     CDS             2323..3090
                     /locus_tag="VH419_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HEY7042638.1"
                     /translation="MGMKTSTARETSGRTRRGFLRGLAAGGAAWGLGAVAAPQTARAA
                     NGDPVLLGVANTATVETEVHMDGLGGALVGRNDDSIGSGVVGMNGASGPGVEAFSAAG
                     SGVLGQSNLGSGVRGVTFLGGPRTAATVGEHKGSGRGVLGVSAAGDGVVGLSEGPGAS
                     IHALAGPGSAGLSLQVDGRAVFARSGKVAVPAGSSSVKVFGVALTESSLVLAMVQQAE
                     GAAFVKWVVPHVVQSSFEIKLNKASSVPVRVAWFVVN"
     gene            3113..3928
                     /locus_tag="VH419_03125"
     CDS             3113..3928
                     /locus_tag="VH419_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042639.1"
                     /translation="MMWSNRLVAGGVLVVLVASMVALSAASGADANDGEPITAGGLTL
                     ESQVTQVANVVISPDCGPGDVGVGLEACGGVGLSGTGIIEGVVGHGAGDFSSGVRGSA
                     NGVQAIGVEATGFGSGSTGVKASGSGADSTGVFGLGGRFGVTGITAGGCIVLHGCAVG
                     VQGTNTSNGYGVQGSSVDGVGVVASSANGTALSVSGKARFSRSGVVSIASGSTSKSVT
                     LAGVTTSSMVLVTAQQNKAVTVKAAVPAAGSFRIFLTGNAPSGGLKVAYFVLN"
     gene            complement(4030..4602)
                     /gene="thpR"
                     /locus_tag="VH419_03130"
     CDS             complement(4030..4602)
                     /gene="thpR"
                     /locus_tag="VH419_03130"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HEY7042640.1"
                     /translation="MSGGDVPLTRLFVALTLPPEVEAHLDETVDGLRTAHEELRWIPS
                     GRWHVTCEFLGECGPHEVERQLGRWERRANRSRPLSLRLSGSGTFPAKAWMARVLWIG
                     LAGDVEAWQQLAAYGQDAHVTIARTRQRHDLTGVVDELAGYAGPSWLAEEVTVMSSIL
                     NPGKSKGSKGGKGTRGPRYVPLATFPLGGP"
     gene            complement(4659..5096)
                     /locus_tag="VH419_03135"
     CDS             complement(4659..5096)
                     /locus_tag="VH419_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020575829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEY7042641.1"
                     /translation="MRISDVLHGKPQGDAVVTVSPDTTVRDLIALLDEHNIGAVIVRS
                     DDNPVAGIVSERDVVRGLVSGLDLLDRNVSTIMTAEVQTARPEDSVHNLMKLMTEHRI
                     RHVPVVAEGELHGIVSIGDVVKSRIGELEFERDQLTSYVSTSQ"
     gene            5239..6039
                     /locus_tag="VH419_03140"
     CDS             5239..6039
                     /locus_tag="VH419_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042642.1"
                     /translation="MHTTSTQPASASTLRITRRPPSPGWVVTVVVTWITFVLLVDADA
                     TLGIQRLLGVATWLVLLGLLARESNLVRVQTVVVVVFASIVEFTFSPLLEVYLYRFHN
                     VPAYVPPGHGLVYLAALAVGRTAFVRTHLRACMLTVLVAGGGYAAYGLVWADRPDVLG
                     AFWYLCLVGFFLWGPSRGLYVGAFLVVTYLELLGTGLGTWTWQPHDPTGWVAIGNPPS
                     GAAGGYGWFDLAALTLAPSVVGVRLLFRRAEPQPASTEIVHPAGQPVA"
     gene            complement(5947..6840)
                     /gene="hisN"
                     /locus_tag="VH419_03145"
     CDS             complement(5947..6840)
                     /gene="hisN"
                     /locus_tag="VH419_03145"
                     /EC_number="3.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HEY7042643.1"
                     /translation="MPTDHTDDLRLAHVLADDADSLTMDRFKAQDLHVVNKPDLTPVT
                     DADKSVEQSIRRTLGRARPRDAFYGEEEGSSGWGPRRWVVDPIDGTKNFVRGVPVWAT
                     LIALMVEDDVVASVVSAPALGRRWWASKGSGAFTGKSLFSASQCHVSDVGTLENASLS
                     YSSLHGWDALGRHDDFVALTRRCWRSRAYGDFWSHMLVAEGAVDLAAEPELELYDMAA
                     LAVIVEEAGGRFTGLDGTPGPHGGNALATNGRLHDQALAFVGVLPGDAESRDRLPGRV
                     HDLGARRLRLSPSEQESDADD"
     gene            7092..>7333
                     /locus_tag="VH419_03150"
     CDS             7092..>7333
                     /locus_tag="VH419_03150"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HEY7042644.1"
                     /translation="MFDEVHGLPLHPLAVHAAVVSVPLAALLGILFAIPKTRAWSRWP
                     LLLVSLGAVGAVYVAKQSGHELEHHLNLQGVTLDLIT"
BASE COUNT         1076 a         2459 c         2604 g         1194 t
ORIGIN      
        1 cggaccggga ggcgcggttc ccatcccgca gtcggtcatg ccgctggtgg cgggacggat
       61 ctccgcgctc ccggccaccg cccgcaaggc gctcgttgcg gctgccgtcc tcgtcgatcc
      121 atccctgccg gccatccggt cgttgacgaa caaccgaggg cgcctcgctg ggctcggctc
      181 agctgaagtg tcaggggtgg tcgagatcaa ggacggccgt gtccggttca cccatccact
      241 gctcgggtcg gcagcgtatg cgtccgcgtc ggaggagcga cgccggcggc tccacctgaa
      301 ggcggctgcg ttggcgatcg accccgagga acgcgcccag cacctcgccc tgacagccaa
      361 gggcccgagc gctgccatcg cggccgcgct ggatgtggcg gctgaatggg ccgagagcag
      421 aggcgctccg gaccgggccg ccgagttgtg ggaggcggct gccgggctca ctcccggcac
      481 acggaccgag ggtaggtggc gccgcgccgt ccgggctgcc gagtgctact tccaatccgg
      541 agacaacgcg tcggccaggc cgctgctgga aggcgtgatc gcggtggccc aggcagggcc
      601 cctgcgagcg gacgccctcc gagcactgag catggtccag cggcgcgacg gcctccgaga
      661 agcagccgaa ggcagcctgc ggcaggcgtc agcagaggcg ggaaacgcga ccgtgacacg
      721 ggcacgcatc gagctctcgc tggcgtacgc ggtcgcttgg cgccgtgggc tggcgctggg
      781 cctcaggcac gcggagacca cccaacgcct gctcgacgaa gcggcagagc cggggttggc
      841 cggcgcggcg acggccttgg tcttccacct cgagtactcc atggggcgag aggtccgctg
      901 ggacaggctg agcgatgctc tcgcgctcga ggccacaacc ctcgagcgag gtcacacgta
      961 cctgccggtg gatgaacggc cttcctactt cgaagcctgg gcactgcgcg aagcggacga
     1021 acaggaccgg gcgcgcgagc gattcgagga actcaggcga acctgtgcgg atcgcggtga
     1081 ggaaggctct ctcgcgttcc tcctgcatct gatggccgag ctggagacgt cagcaggaaa
     1141 cttctcgctg gcggcacgcc atgcggacga agccaccgag gtcatggtgc gatcagggct
     1201 caggcagatg gagtcggtgt cgctgtacgt ccgggcgcac ttggaggctc ggcagggaaa
     1261 ggtcgccgcc gcgaggacgc atgcgacgcg tgccctggag atcgcgaccc gtgccggtag
     1321 cgtgccgtcc atccacgatg cggccggcgt gctgggtttc ctggcgctgt cgatcggcga
     1381 ccatctcgcc gcggaccggc tgctgacacc ggcgatcacc atgatggtct ctctcggggt
     1441 gggcgatccg gccgcccttc ggtccgtccc cgacgagatc gaggcgctgg tcacgctcgg
     1501 tgagctggga agggccgagg cgctgctgga tcccttcgag cagcaggcct cgttcatggg
     1561 gaggcgctgg gcggcggccg cggcgagcag atgtcgggcc ttggtctgct ccgggcatgg
     1621 cgacctggcc gccgcagtcg gcgctgcgga ccgggccgtc gacgggcacc gcgaactccc
     1681 gtacccattc gagctcggcc ggtcgcttct cgtgcagggt gtggtgcacc ggcgcaggaa
     1741 ggagaaggcg gtcgcggcgg aagccttccg ggaggcggtg gagatcttcg accgactggg
     1801 tacgccgttg tgggcggaca aggctcgagc cgagatccgc agagtcgggc ttcgtccacg
     1861 agctccgctg gagctgaccg ccaccgaggc gcgggtggcc gagctggcgg cgtccggcct
     1921 gaccaaccgg gaagttgcgg atgcgctctt cgtcagtccg aagaccgtcg agtccaacct
     1981 ggccaaggtc tatcgcaagc tcggtatccg ctcgcgcgcc gaactcggcg ccacgatcgc
     2041 agcccgcgaa cgcgtagccg ccaagtctcg atctggctga cccctcgcgg ccgcctcgtg
     2101 tctacgttcg agagcggagc aggtggagcg aggcgccagt gccgacgtac ctggtcgaga
     2161 gctggccggg gatcagtcag acacgtgcgt gcccggttcg gagccggcag acggaattga
     2221 gacggatcag gcgccgtcac agacgtaggg aaacacccga ttccaagcgt cccggacggc
     2281 actacggtcg ctgtggccgg gttccgacga agggcagctg tgatgggcat gaagacgtcg
     2341 acggcacgag agacgtctgg tcgcaccagg cgtggattcc ttcgtgggct ggccgccggg
     2401 ggagcggcct ggggtcttgg cgccgtcgcc gcaccgcaga ctgcccgcgc cgcgaacggc
     2461 gacccggtgc tcttgggcgt cgcaaacacc gccacggtcg agaccgaggt ccacatggac
     2521 gggctcggcg gcgcactggt ggggaggaac gacgacagca tcggctccgg tgtcgtgggc
     2581 atgaacggcg catccggacc cggtgtggag gcgttctctg cggcggggag cggcgtactc
     2641 gggcagtcga acctcgggtc cggcgtgcgg ggcgtgacgt tcctcggtgg cccacgcacc
     2701 gcagccaccg tgggcgagca caagggttcc ggtcgaggag tgctgggggt ctcggcggcg
     2761 ggtgatggag tcgtgggact gtcagaaggc ccgggggcga gcatccacgc gctggccggg
     2821 ccgggcagtg ccgggctgtc gctccaggtc gacggacgcg cggtgtttgc caggagcgga
     2881 aaggtcgcgg tgccggcagg ctcgtcgtcg gtgaaggtct tcggtgttgc cctgaccgag
     2941 tcgagcctcg tgctggccat ggtgcagcag gcggagggcg cggcgttcgt gaagtgggtg
     3001 gttccccacg tcgtccagtc gtcgttcgag atcaagctga acaaggcgtc gtccgttccg
     3061 gtgcgggtgg cctggttcgt cgtgaactga ctcgaggaag tggggtgtcg gcatgatgtg
     3121 gagcaatcga ctggtggccg ggggtgttct ggtggtcctc gtcgcctcca tggtggcgct
     3181 ctcggcggca tccggtgctg acgcgaacga cggagagccc atcacagcag gtggcctcac
     3241 gctcgagtcg caggtgaccc aggtggccaa cgtcgtcatc tcccccgact gcggtcccgg
     3301 cgacgtcggc gtgggccttg aagcctgcgg gggcgtcggt ctgtcgggga ccggcatcat
     3361 cgagggcgtc gttggccacg gcgcgggcga cttctccagt ggcgtccgcg gttccgccaa
     3421 cggtgtccag gcgatcggcg tcgaggcgac cggtttcggg tcgggctcga ccggcgtcaa
     3481 ggcgtccggc tcaggagccg actcgaccgg cgtcttcggc ctgggcggca ggttcggcgt
     3541 caccggcatc accgctggtg gctgcatcgt gttgcacgga tgcgctgtcg gcgtacaagg
     3601 caccaacacc agcaacgggt acggcgtgca ggggtccagc gtcgatgggg tcggggtcgt
     3661 ggcgtccagt gcgaacggca ccgcgctgtc ggtgagcgga aaggccaggt tcagccgaag
     3721 cggagtcgtc agcatcgctt ccgggtcgac gtccaagtcg gtgacgctgg ccggtgtgac
     3781 cacctcgagc atggtcctgg tcaccgccca gcagaacaag gctgtgacgg tgaaggccgc
     3841 ggtcccggcg gccgggtcgt tcaggatctt cctgacggga aacgcgccgt ccggaggcct
     3901 gaaggtggcg tacttcgttc tcaactgatc gatgcgcggg aagcgtcgat cgcaaacgac
     3961 ctgtccccat cggaggtttg cgatgtttac agcccttccg ggctgtaaac atcgcaaacc
     4021 ccacagcggt cacggaccgc cgagggggaa ggtggcgagc gggacgtagc gcgggccgcg
     4081 agtacccttg ccgcctttgg agcccttgct cttgccgggg ttgaggatcg acgacatcac
     4141 ggtcacctcc tccgccagcc agctcggccc ggcgtacccg gcgagctcgt cgacgacccc
     4201 ggtgaggtcg tgccgctgtc gggtgcgggc gatcgtgacg tgcgcgtcct gcccgtacgc
     4261 cgccagctgt tgccacgcct cgacatctcc ggccaggccg atccagagca cgcgcgccat
     4321 ccacgccttc gcggggaacg tgccggaccc ggacagccga aggctcagcg gccggctccg
     4381 gtttgcccgg cgctcccacc ggccgagctg acgctcgacc tcatgcggcc cgcactcgcc
     4441 caagaactcg cacgtcacgt gccaacgccc cgacgggatc cagcgcagct cctcgtgcgc
     4501 cgtgcgcagc ccgtcgaccg tctcgtcgag atgagcctcg acctcgggcg gcaacgtcaa
     4561 cgcgacgaac agccgggtca gcgggacgtc acctcctgac acgtccggcc agcagtggag
     4621 gctccgcggt ggccagtcgg acgaatcgtg gcggtcgcct actgggaggt cgagacgtac
     4681 gacgtcagct ggtcgcgctc gaactccaac tcgccgatcc gggacttcac cacgtcaccg
     4741 atcgacacga tgccgtgcag ctcaccctcc gcgacgaccg gcacgtggcg aatccgatgc
     4801 tcggtcatca gcttcatcag gttgtgcacg gagtcctccg gcctcgctgt ctgcacttcg
     4861 gctgtcatga tcgtggacac gttgcggtcg agcagatcga ggccggacac gagtccgcgg
     4921 acgacgtcac gctccgacac gatgcccgct accgggttgt cgtcggaccg cacgatgaca
     4981 gcaccgatgt tgtgctcgtc gaggagggct atcaggtcac gtacggtggt gtcggggctc
     5041 acggtcacga cggcgtcccc ttgtggcttc ccgtgcagta cgtcgctgat ccgcatcgtg
     5101 tcctcccagg agtctcgatg tacccacgac agtagccgtg cgtgacggtg cgcgataccc
     5161 catgcgtacc gccgcccgtt tcggtcctcc cgccccgttc cacatggtgg gtttcgatgg
     5221 acctgcgaag atgtcggcgt gcacaccacg tcgacgcagc cggcctccgc aagcacgttg
     5281 cggatcacgc gtcggccgcc ttcgccgggc tgggtggtga cggtcgtcgt cacgtggatc
     5341 acgttcgtcc tcctcgtcga cgccgatgcc accctcggca tccaacgtct cctcggagtg
     5401 gcgacgtggc tcgtgctgct cggtctgctc gcacgtgagt cgaacctggt gcgggtccag
     5461 accgtggtcg tggtcgtgtt cgcgtcgatc gtcgagttca cgttctcgcc gctgctcgag
     5521 gtctacctct accgctttca caacgtgccg gcgtacgtgc cgcccggtca tggcctcgtc
     5581 tacctcgcgg ccctcgcggt tggccgcacg gccttcgtgc gcacacacct ccgtgcctgc
     5641 atgctcaccg tcctcgtcgc gggtggcggc tacgcggcgt acggcctggt gtgggcggac
     5701 cgcccggacg tgctcggggc gttctggtac ctgtgcctcg tcggcttctt cctctggggt
     5761 ccctcccggg gtctgtacgt cggcgcgttc ctcgtcgtca cctatctcga gctgctcggg
     5821 accgggctcg gcacctggac ctggcagccg cacgacccga ccggctgggt ggcgatcggg
     5881 aatccgccgt cgggcgcagc cggcggctac gggtggttcg acctggcggc gctcacgctc
     5941 gccccgtcag tcgtcggcgt ccgactcctg ttcagacggg ctgagccgca gcctgcgagc
     6001 accgagatcg tgcacccggc cgggcagccg gtcgcgtgac tcggcgtcgc caggcagcac
     6061 gccgacgaac gccaacgcct ggtcgtgcag gcgaccgttc gtcgcaaggg cattgccgcc
     6121 gtgcggtccg ggcgttccgt cgagcccggt gaagcggcca ccggcctcct cgacgatgac
     6181 ggccagcgcg gccatgtcgt acagctcgag ctccggctcg gccgcgagat ccaccgcgcc
     6241 ctccgccacg agcatgtggg accagaagtc gccgtacgcc ctgctgcgcc aacagcgacg
     6301 cgtcaatgcg acgaagtcgt cgtgccggcc gagcgcgtcc cagccatgga gcgaggagta
     6361 cgacagcgac gcattctcca aggtcccgac gtctgacacg tggcactggc tcgcggagaa
     6421 caacgacttg ccggtgaacg caccacttcc cttcgacgcc caccatcgac gtccgagcgc
     6481 cggcgccgac acgacgctcg caacgacgtc gtcctccacc atcaaggcga tgagggttgc
     6541 ccacaccggg acgccgcgaa cgaagttctt cgtgccgtcg atcgggtcca ccacccaccg
     6601 tcgcggcccc cagcccgacg agccctcctc ctcgccgtag aacgcgtcgc gcggacgagc
     6661 ccggccaagc gtccggcgta tcgactgctc gaccgacttg tcggcgtcgg tgacgggtgt
     6721 cagatcgggc ttgttgacga cgtggagatc ctgcgccttg aaccggtcca tcgtgagcga
     6781 gtccgcgtcg tcggcgagca cgtgcgccag acgcagatcg tccgtgtggt cggtgggcat
     6841 agcgatcagc gtacggcgcc acgaccatcc ctcctcgtca ggctcgcggt gaaccgttcg
     6901 acgtcctgaa ctgtcggtga gacgaggcgt gccggctcgg acggcgactc atgcccgaag
     6961 gtccaatggg gagcgttgcg tgctcgccgc gagactggaa aggccgatag cgtcttgacg
     7021 tcgaccgtgg cggacgcctt cgtaccaaac ggccgagggt actcacggtc ccgagcgatc
     7081 gaggtctgca gatgttcgac gaggtccacg gtcttcccct ccacccgttg gcggtgcacg
     7141 cggccgtcgt ctctgtcccg ctggcggcgc tgctcggcat cctgttcgcg atcccgaaga
     7201 cgcgtgcgtg gtcgcggtgg ccgctgctgc tcgtctccct cggcgcggtc ggtgcggtgt
     7261 acgtcgccaa gcagagtggg cacgagctcg agcaccacct caacctgcag ggcgtaaccc
     7321 tcgacctgat cac
//
LOCUS       DATBFZ010000106        13333 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_1046686, whole genome shotgun sequence.
ACCESSION   DATBFZ010000106 DATBFZ010000000
VERSION     DATBFZ010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 13333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13333
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1046686"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2528)
                     /locus_tag="VH419_03155"
     CDS             complement(<1..2528)
                     /locus_tag="VH419_03155"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HEY7042645.1"
                     /translation="MTVSDGSVVATADATAPYLRPAADVVADLQTDLASGLSAQEAAA
                     RLASHGPNQITTEKPPSPFAIALGQLRDPMNIMLVAVAVVSLVIGQGSTALLVALLVL
                     LNVVLGTRQELKAQASVDALSKLQVPQTRLVRDGTVVLVPAVDVVPGDVVQVEAGDIV
                     PADGRIATSATLETQEAALTGESAPVSKDAAVLTGPDVSVGDRTNMLFQNTSVTRGTA
                     TMVVTATGMQTQMGEIATMLTSVKRTRSPLQKELDSLTGVLGVIAWTAVALIIVVGLL
                     RDLPLDDVLLLGTAMAISAIPTGLPTFVQAMLSYGAKQLAEAKAVVKNLTDVETLGAT
                     SAINTDKTGTLTMNQMMVSTIYAGGSWFTVDGEGYSKSGNILSVAGTPVPDFTRLALG
                     LALDSDATVSDAGAVVGDPTEAALVVLAAKLGVDAEETRRTYPRLAEVPFDSEYKFMA
                     TFHRVTVDGTEHLIELVKGAPDVVLARCSRAGGPLSGSQAPIDEQRAAIETANEDLGA
                     KGLRVLAFAARELSESDVEALSVDPMAFTTQLGFVAMVGIIDPLRTEAKGAVTTALAA
                     GIDVRMITGDHAVTARAIGETLGLGPGAVSGAELRAMTDDELRQRLPDLHVFGRVAPE
                     DKLRLAQVMQGEGMIVAMTGDAVNDAAALKQADIGVAMGTGSEVSKQAARMILTDDNF
                     GTLVHAIELGRTIYSKIVSYIRYQMSQLLCLVMLFVTATVFNINDGVAMTPLMVLFLN
                     FFVCIFPVIVIMLDPGDPDVMRLPPRDPKVPITNRAAMIRWVLYGAVFFLAALVPLVW
                     GPDDLSTDEPSASMTMSFVVVGLGTVFSGLAMRRDPTSGLLPPIL"
     gene            complement(2525..3493)
                     /gene="arcC"
                     /locus_tag="VH419_03160"
     CDS             complement(2525..3493)
                     /gene="arcC"
                     /locus_tag="VH419_03160"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002527839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEY7042646.1"
                     /translation="MRIVVALGGNALQRRGEPMTMQRQRANVAAACKALAPVALEHEL
                     VISHGNGPQVGLLALQASSYDEASAYPFDVLGAQTEGMIGYLIEQELGNLLPFEKPLV
                     TILSMTEVDPDDPAFADPTKFVGPVYSEEDAKRLAAERGWTVRPDGDMWRRVVPSPLP
                     QRIFESRAVEWLVEKGCVVVCAGGGGIPTMYTKGTRTLVGVEAVIDKDRASAVLAEGL
                     KADLLVIATDVDAVYVDWGTPDQRAVASAAPDALAALPLPAGSMGPKVEAATAFATGT
                     GKPCVIGALDALSGILAGSSGTRVSIDIQDIEFRPAAGSATTGETP"
     gene            complement(3527..4534)
                     /gene="argF"
                     /locus_tag="VH419_03165"
     CDS             complement(3527..4534)
                     /gene="argF"
                     /locus_tag="VH419_03165"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005318430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEY7042647.1"
                     /translation="MAVNLRHRDFLKELDFTRTELQYLIDLSRDLKRAKYSGTEVPRM
                     RGKNVALIFEKTSTRTRCAFEVAAYDQGGHVTYLDPTSSQIGHKESAADTARVLSRMF
                     DAIEFRGKAQSTVEELAEYGDVPIYNGLTDEWHPTQMLADFLTMSEHADGKPLHEVSY
                     CFMGDGRFNMGRSLLVMGAIMGSDVRICAPEDLWPDKDVQDVAHERAEESGAKVTLTA
                     DPEEGLSGADFVHTDVWVSMGEPKDVWSSRVAELTPYQVNAKAMSRTGNARAKFMHCL
                     PAFHDTRTVVGKDVADSTGMTEGLEVTDEVFSSPANIAFDQAENRLHTIKALMVATIG
                     A"
     gene            complement(4596..5834)
                     /locus_tag="VH419_03170"
     CDS             complement(4596..5834)
                     /locus_tag="VH419_03170"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: protein motif:HMM:NF002381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEY7042648.1"
                     /translation="MSFHVDSEVGVLRQVILHRPGLELTRLTPSNVHDLLFDDVMWAQ
                     RAREEHDAFAQKLRDKGVKVYLFVDLLAEALEQPGSREFLQEELTSATRFGPALDRPL
                     DDLVAAAPADRLAELLVGGVLRSDLDLSETDSLLLKYLDPDDFLLTPLPNHLFQRDNS
                     AWVYDGVSVNPMSKQARKRETINSRLLYNYHSMFSKSDPPVHFYYGNDSKDHEPANCE
                     GGDILVIGNGAVMIGMGERTTPQGIEFLAREYFSHGKVTKIIAVELPKTRAFMHLDTA
                     MTMIDRDAFSVYPHLPDTLRSFTLEPVGSGGGLGDYTVTENDELFPVVADALGVEKLR
                     VLRTPIDVAGAQREQWDDGNNFLAVAPGVIFGYERNTTTNTFLRKNGIEIVTITGSEL
                     GRGRGGPRCMSCPIERDAVA"
     gene            6077..8728
                     /locus_tag="VH419_03175"
     CDS             6077..8728
                     /locus_tag="VH419_03175"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEY7042649.1"
                     /translation="MTPAGDETGVGPRIVPTSSLLRSKLRSPVPPEHYVRRSRLLELL
                     DDMVRRPLTLVVAPAGAGKTSMLADWLARSPMPRSWLSLDETDRDRAHLWLGITVAVD
                     ALVPGCAADAIEALQRPGGAADAVQVLLSSLDEVAAEPKVLVMDDVHCIDDSAEAVES
                     LGTFLQHLPGWLRVVVVGRRAPRLPIDRLRARGQLSEIRFAELRFSEAEAVDLLSRLS
                     PGMAVEELSEATEHAGGWAAGLQLAALAARSTRAQADSFHAPRHDDSLVEDYVWHEVL
                     THESPELVDVLLNTSVVERVNRSLAIALTGRTDAEQLLRSAEERGLFVTRLGSTGWFE
                     VHSLVREILLSELFRRSPGILDTLHQRAAQWYEEAGEETAGLDHWLAGNRPREALRLL
                     AASEAKLYDSGRESTITRVITRIPVSVSAADLEAQLEFAWCHLLVDRHRFLEYVAQAV
                     DVSRQTRGVDKVLRGRVAMLQSIAATMVGDWHEGGRQAREAMSELGEAGWLDLLGRFG
                     WNMVARDIALSERWHDAADEVREVRTALSVDPERRLAFEGTRAVGLAMAGQPLDAVRA
                     AAGVRSSAEVAHLAILRGELAIADALTHRELGDRQRARAELTAIVDRPVEPLTYARGL
                     AWLELSQLWLDEGDLGAAQAAFRGAEAFADAEQHGSGALDWLARVGTVVALAAGDMPA
                     AKAWCERVQDPFWRPVSLARVHLAVGDKPAADDALERVAARCPRHEVVRDLLRARATA
                     HHEDAVKYATAAVELASAHGMLQTVLSEGDDVQQLVELASWRAPSAWLGRFRRGVPSL
                     VHAARPVVAHPDALTDRERDVLRLLPSRLTQREIAGELSISLNTLKFHLKVIYRKLGV
                     NSRGDAARLARDVLSGPRVQSPNTFER"
     gene            complement(8701..8904)
                     /locus_tag="VH419_03180"
     CDS             complement(8701..8904)
                     /locus_tag="VH419_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042650.1"
                     /translation="MAWTSRPRGTYPDLLIRCPLSERTLPRRSTAVLVNQVEQVRELA
                     KLVDRGLVSRQEFELQRSKVFGL"
     gene            complement(8917..9387)
                     /locus_tag="VH419_03185"
     CDS             complement(8917..9387)
                     /locus_tag="VH419_03185"
                     /inference="COORDINATES: protein motif:HMM:NF039728.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6325 family protein"
                     /protein_id="HEY7042651.1"
                     /translation="MTGTRVTGNAGESMSIDTNLVEYVIVSMPQVGDLSLLAPPLGRL
                     VQDRTLRVLDLVAVETTLEGSVQVIEPEYIPGLAALAGSVGIGDGILSERDLLLASTV
                     VRPGSAAIIVVVEDLWARRLSNAAKRAGGNVIAGERIPQSRLEAALARVSPGKD"
     gene            complement(9428..9823)
                     /locus_tag="VH419_03190"
     CDS             complement(9428..9823)
                     /locus_tag="VH419_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017934235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HEY7042652.1"
                     /translation="MTVAADFGVGDVFLSMLWFFLFFLWIWLLIAVFAHIFRSEDLSG
                     WGKALWTVFVIVLPYIGVLVYLIVRGSAMGEDQRRHAQAHEKAMREYVQSAAGTGGGA
                     SEISQLAALRDKGIITDAEFEQAKAKILS"
     gene            10098..11012
                     /locus_tag="VH419_03195"
     CDS             10098..11012
                     /locus_tag="VH419_03195"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEY7042653.1"
                     /translation="MRKKLTFVSLLALLVLPLAACGGDDSGDNDSSAPPAGSGVCGSN
                     KTTDDLLAEICDKGVITVSTDPKYPPQSSLNEQTGKYEGFDIDVATEIANRLGVKVAW
                     QTPAWEAITAGSWNGRWDMSVGSMTPTNDRQKVLDFSQPYYFTPAVVVVHKDNTSVQD
                     IATDLDGKKIGVCSACTYEQFLKKTLAIKGFTFDFVIDDAEAVGYDTDTSALNDLALG
                     DGVRLDAVITSLTTAQGFIDSGKPVKIVGDPVFYEPLAVAIDKSAPKDPSSLAAAVDK
                     IVGEMHADGTLTTMSKKWYDGTDLTVTQ"
     gene            11029..11970
                     /locus_tag="VH419_03200"
     CDS             11029..11970
                     /locus_tag="VH419_03200"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEY7042654.1"
                     /translation="MSRSDDTLLDEPTSRWERLALTSAGIPFRLKVAAVWVVIFLILA
                     GFFAIADFDTGFMKDEFPFIVKGLRYTLYMAVVGIALAVVLAALGALGRISSNPIAYG
                     VSGFYVSFFRGTPLIVQMTLIYSALPQIGFNLVAKYPSVPDQLASFLTLQAVVAGMIA
                     LGLNYGAYMTEIFRAGIQSVASGQGEAADALGMTYRQKMRKVVLPQAFRVIIPPTGNE
                     FIAMMKDTALVSFLGTALTSAEIFRRAQYVGTTHFRSLEALVVAAGLYWSLTVIFTFF
                     QRRLETRIGRGYVRTVGVKRPGSVAQPHAQGAGQVEL"
     gene            11967..12737
                     /locus_tag="VH419_03205"
     CDS             11967..12737
                     /locus_tag="VH419_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004811951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEY7042655.1"
                     /translation="MSERDVRDVIVRVDGLHKYFGHLEVVRGVDMEVRKGEVVVIFGR
                     SGSGKSTLLRCVNFLEDPTAGTVEVAGIRLRGGHRTRRKRERIRQLRLNVGMVFQQFN
                     LFPNMTALDNVMSGPLSAKRGRESQIKERALDLLDQVGLSDKAQEHPIRLSGGQQQRV
                     AIARALAMDSEVMLFDEPTSALDPELTGEVLGVMKSLATELHMTMMVVTHEMGFAREV
                     ADRMCFFHEGVILEEGAPADMVSSPRFPETKQFLDAVL"
BASE COUNT         2214 a         4370 c         4486 g         2263 t
ORIGIN      
        1 agtatcggtg ggagcagacc gctggtcggg tctcgtcgca tcgccagccc gctgaagacc
       61 gtgcccaacc cgacgacgac gaagctcatc gtcatcgagg cgcttggttc atcggtactc
      121 aggtcgtcag gtccccagac gagcggcacc agtgctgcca ggaagaacac tgcgccgtac
      181 agcacccacc ggatcatggc tgcccggttc gtgatcggca ccttcgggtc gcgtggcggg
      241 agccgcatga cgtctgggtc gccagggtcg agcatgatga cgatcacggg gaagatgcag
      301 acgaagaagt tgaggaacaa gaccatgagg ggcgtcatcg cgacgccgtc attgatgttg
      361 aagacggtcg ccgtgacgaa cagcatcacg aggcacagga gctgtgacat ctggtatcgg
      421 atgtacgaca cgatcttgct gtagatggtg cggccgagct cgatggcgtg caccaacgtg
      481 ccgaagttgt catcggtgag gatcatccgc gcagcctgct tggacacctc gctgccggtg
      541 cccatggcca cgccgatgtc ggcctgtttg agcgccgccg cgtcgttgac cgcgtcgccg
      601 gtcatcgcga cgatcatgcc ttcgccctgc atgacctggg cgagtctcag cttgtcctcc
      661 ggtgcgacgc gaccgaacac atgcaggtcg ggaagccgct gacgaagctc gtcgtccgtc
      721 atcgcgcgaa gctcggcgcc actcaccgca cccggaccga gaccgagggt ctcgccgatg
      781 gcccgcgccg tgactgcgtg gtcaccggtg atcattcgca cgtcgattcc agcagcgagc
      841 gcggtcgtga ctgcaccctt tgcctctgtg cggagagggt cgatgatccc gaccatggcg
      901 acgaagccga gctgtgtggt gaacgccatc gggtcgaccg acagggcctc gacatcgctc
      961 tcgctgagct cacgcgcggc gaaggccagc acacgcaagc ccttcgcgcc caggtcctcg
     1021 ttggccgtct cgatggcggc gcgctgttcg tcgatcggag cctgggaacc gctcagtgga
     1081 ccgccggccc ggctgcatcg ggccagcacg acgtcgggag cacccttgac gagttcgatc
     1141 aggtgttcgg tcccgtcgac ggtgacccgg tggaaggtgg ccatgaactt gtactcggag
     1201 tcgaacggca cctcggcgag cctcggatag gtgcgccggg tctcctctgc gtcgacaccg
     1261 agcttggcgg ccagcacgac gagcgccgcc tccgtcggat ctcctacgac cgcgcctgcg
     1321 tcgctgacgg tcgcgtcgct gtccagagcc agacccagcg ccagccgggt gaagtccggc
     1381 accggtgtcc cggcaaccga caagatgttg cccgacttgc tgtagccctc accgtcgacg
     1441 gtgaaccagg atccgccggc atagatcgtc gacaccatca tctggttcat cgtcagggtg
     1501 ccggtcttgt cggtgttgat cgcgctggtc gcgccgaggg tctccacgtc ggtcaggttc
     1561 ttgacgacgg ctttcgcctc ggcgagctgc ttggcgccat acgacagcat cgcctgcacg
     1621 aaggtcggca gaccggtggg gatcgccgag atggccatcg ccgtccccag cagcaagacg
     1681 tcgtcgagcg ggaggtcacg gagcagaccg accacgatga tgagcgccac cgcggtccac
     1741 gcgatcacgc cgagcacacc tgtcagtgag tcgagctcct tctgcagcgg cgaccgggtg
     1801 cgcttgaccg acgtgagcat ggtggcgatc tcacccatct gggtctgcat gcccgtcgcc
     1861 gtcaccacca tcgtcgcggt gccgcgggtg accgatgtgt tctggaagag catgttggtg
     1921 cgatcgccga cagagacatc aggtccggtg agtacggctg cgtccttgct caccggcgcg
     1981 ctctcaccgg tgagtgctgc ctcctgcgtc tccagcgtcg cggacgtggc gatacggcca
     2041 tcagcaggga cgatgtcacc agcctcgacc tgcacgacgt cgccgggtac gacgtcgacc
     2101 gccgggacga gcacgaccgt gccgtcccgg acgagacggg tctgcggaac ctgcagcttc
     2161 gacaaggcgt cgacgctcgc ctgcgccttg agttcctgcc gcgttccgag gacgacgttc
     2221 agcaggacga gcagtgcaac gagcagcgcg gtcgagcctt ggccgatgac gaggctgacg
     2281 accgcgacag cgaccagcat gatgttcatc gggtcgcgaa gctggccgag tgcgatggcg
     2341 aacggcgacg gaggtttctc cgtggtgatc tggttcggcc cgtggctggc cagtcgggcg
     2401 gcagcctctt gggcggacag gccggacgcg agatcggtct gcaggtcggc gacgacgtcg
     2461 gcggccggcc tcaggtaggg cgcggtggcg tcggccgtcg cgaccacaga gccgtcgctg
     2521 acggtcatgg tgtttctccc gtcgtcgcag agccagccgc gggccggaac tcgatgtcct
     2581 gtatgtcgat cgagaccctc gtccctgagg agccggcgag aatcccggac agcgcgtcga
     2641 gtgccccgat cacgcacggc ttccccgtgc cggtcgcgaa ggccgtcgcg gcctccacct
     2701 tgggtcccat cgacccggcc ggcaggggga gggcggccag tgcgtccggg gccgccgatg
     2761 cgaccgcccg ctgatcggga gtgccccagt cgacgtagac cgcatcgaca tcggtcgcga
     2821 tcaccagcag atcggccttc agcccctcgg cgagaaccgc gctcgcccgg tccttgtcga
     2881 tgaccgcttc aacgcccacc agcgtgcggg tgcccttcgt gtacatggtc gggatgccgc
     2941 cgccgcccgc acagacgacg acgcacccct tctcgaccag ccactcgaca gctcgcgact
     3001 cgaagatccg ctgcgggagc ggagacggca cgactcgccg ccacatgtcg ccgtccggcc
     3061 gcaccgtcca gccgcgttcg gccgccagcc gcttggcgtc ctcctcggag tacaccggcc
     3121 cgacgaactt ggtcggatcg gcgaacgcag ggtcgtccgg gtcgacctcg gtcatcgaca
     3181 ggatcgtcac cagcggcttc tcgaacggca gcaggttgcc gagctcctgc tcgatcaggt
     3241 agccgatcat cccctcggtc tgtgcaccca gcacgtcgaa cggataggcg gaagcctcgt
     3301 cgtacgacga tgcctgaagc gcgagcaggc cgacctgcgg gccgttcccg tgcgagatga
     3361 ccagctcgtg ctccaaggcg actggggcaa gcgccttgca ggcggcggcg acgttggccc
     3421 gttgccgctg catcgtcatc ggctcacctc gacgctgaag ggcgttcccg cccagcgcca
     3481 ccacgatcct catcggccct cccgccggct cgaggtcgac gccgctctag gcgccgatgg
     3541 ttgcgaccat gagtgccttg atggtgtgca gccggttctc tgcctggtcg aacgcgatgt
     3601 tcgcaggcga agagaacacc tcgtcggtga cctcgaggcc ctcggtcatg cccgtcgagt
     3661 cggcgacgtc cttgccgacg accgtccgcg tgtcgtggaa ggccgggaga cagtgcatga
     3721 acttcgctcg tgcattgccg gtgcgggaca tggctttcgc attcacctgg tacggcgtga
     3781 gctcggccac ccggctcgac cagacgtcct tgggctcgcc catcgagacc cacacgtcgg
     3841 tatgcacgaa gtcggctccg ctcaggccct cttcggggtc ggcggtcagc gtcaccttcg
     3901 cgccggattc ctcggcccgc tcatgggcga cgtcctggac gtccttgtcg ggccagagat
     3961 cctcaggagc gcaaatgcgc acgtcggatc ccatgatggc gcccatcacg agcagcgaac
     4021 gtcccatgtt gaagcgcccg tcgcccatga agcagtagct gacctcatgg aggggtttgc
     4081 cgtcggcgtg ctctgacatg gtgaggaagt ccgcgagcat ctgggtgggg tgccattcgt
     4141 cggtgaggcc gttgtagatc ggtacgtcgc cgtactcggc aagctcctcg acggtggact
     4201 gtgccttgcc gcggaactcg atcgcgtcga acatgcgtga caacacccgt gccgtgtccg
     4261 cggcagactc cttgtgtccg atctgcgacg acgtcgggtc caggtaggtc acgtgaccgc
     4321 cctggtcgta cgccgccacc tcgaaggcac agcgcgtccg ggtggaggtc ttctcgaaga
     4381 tgagcgcgac gttcttgcct cgcatccgcg gaacctcggt gccggagtac ttggcgcgct
     4441 tgagatctct ggacaggtcg atcaggtact gcagctcggt gcgggtgaag tcgagctcct
     4501 tcaggaagtc tcgatggcgg aggttgacgg ccatctgtgc tcaccggcct ttctcggtca
     4561 cggtccggaa cttgatctgg gagtggggcg gtcggtcacg cgacagcatc gcgctcgatc
     4621 gggcaggaca tgcagcgagg tccaccgcgg cctcggccga gctcactgcc ggtgatcgtg
     4681 acgatctcga tgccgttctt gcgcaggaac gtgttggtcg tggtgttccg ctcgtacccg
     4741 aagatcacac cgggcgcgac cgcgaggaag ttgtttccgt cgtcccactg ctcgcgctgc
     4801 gcgccggcca cgtcgatcgg cgtccgcagc acgcgcagct tttcgacccc gagggcgtcg
     4861 gcgaccacgg ggaagagctc gtcgttctcc gtcaccgtgt agtcgccgag gccgccgccg
     4921 ctgccgaccg gctcgagggt gaaggagcgc aacgtgtccg gcaggtgcgg atagacgctg
     4981 aacgcgtcac ggtcgatcat ggtcatcgcg gtgtcgaggt gcatgaacgc tctggtcttc
     5041 ggcagctcga ccgcgatgat cttcgtcacc ttcccgtggg agaagtactc gcgagccagg
     5101 aactcgatgc cttgcggcgt cgtgcgctcg cccatgccga tcatcaccgc gccgttgccg
     5161 atgaccagga tgtcgccgcc ctcgcagttg gcaggctcgt ggtccttgct gtcgttgccg
     5221 tagtagaagt gcaccggcgg atcgctcttg ctgaacatgg agtggtagtt gtagagcagc
     5281 cgcgagttga tcgtctcgcg cttgcgtgcc tgcttgctca tcgggttgac cgacaccccg
     5341 tcgtagaccc aggccgagtt gtcccgctgg aagagatggt tcggcagcgg ggtgagcagg
     5401 aagtcatcgg ggtccaggta cttcagcaag aggctgtcgg tctcgctcag gtccagatcg
     5461 ctgcgcaaca cgcccccgac cagcaactcg gccagccggt ccgccggcgc ggccgcaacc
     5521 aggtcgtcca gcggccgatc aagggcgggt ccgaagcggg ttgcactggt cagctcctcc
     5581 tggaggaact cacgagagcc cggctgctcg agggcctcgg ccagcaggtc gacgaagagg
     5641 tagaccttca cccccttgtc gcgcagcttc tgtgcgaacg cgtcatgctc ctcgcgggcc
     5701 cgttgggccc acatcacatc gtcgaacagc aggtcgtgca cattgctggg ggtcagtcgc
     5761 gtcagctcaa ggccagggcg gtgcaggatc acctggcgaa ggacaccaac ctcggagtcg
     5821 acgtggaacg acatgtcggt cacctcccgc tgacgggttc gacccgcctc gacgaatcag
     5881 tcttcaaaca gcatccgaga cttcgccctg gtttgtcgag agcatggtgg ggcggcgtac
     5941 cggagcacct cctgaaacca ccgtcgcctg gacgcgggtg gtcgtgcacc acccccgaac
     6001 gggtggaagg cccggacgac gacgcgacac aggtaatctc atgttcggag ggggcggtag
     6061 aggggctgga ggcagcatga cgcccgccgg cgacgagact ggggtgggtc cgcgcattgt
     6121 tcccacgtct tcgctgctgc gctcgaagtt gcgcagcccc gtaccgccgg aacactacgt
     6181 acgacggtcg cggctgctgg aactgctcga cgacatggtg aggcgaccgc tgacactggt
     6241 tgtcgctccc gccggcgcgg gcaagacgtc gatgcttgcg gactggctgg cccgatcacc
     6301 gatgccgcgc tcgtggctgt cgttggacga gacggaccgg gacagggcgc acctgtggct
     6361 gggaatcacc gtcgcggtcg acgcgctggt gcccggctgt gccgcggacg ccatcgaagc
     6421 gctgcagcgt ccgggcggag cagcggacgc ggtccaggtg ctgctctcgt cacttgatga
     6481 ggtggctgcg gagccgaagg tgcttgtcat ggacgacgtg cactgcatcg acgacagcgc
     6541 ggaagccgtg gaatcgctcg gcaccttcct ccagcacctt ccagggtggc ttcgtgtcgt
     6601 cgtggttggt cgccgcgcgc cgaggctacc gatcgaccgg ctgcgtgcgc gtggccagct
     6661 gagcgagatc aggttcgcgg agctgcggtt ctccgaggcc gaagctgtcg atctgctctc
     6721 cagactctcc ccggggatgg ctgtcgagga actgtccgaa gcgaccgaac acgccggcgg
     6781 atgggcggcc ggactgcagc tggcagcgct cgcggcacgt tccacccgcg cccaggctga
     6841 ttccttccac gcgcccaggc acgacgactc cctggtcgag gactacgtat ggcacgaggt
     6901 gctgacccac gagtcgcccg agctcgtcga cgtactcctg aacacctcgg tcgtcgagcg
     6961 ggtcaaccgg agcctcgcga tcgcgctcac cggtcggacc gacgcggaac aactgctgcg
     7021 atccgccgag gaacgcgggc tgttcgtgac tcgactcggg agcaccggtt ggttcgaagt
     7081 gcactcgctc gtgcgggaga tcctcctgtc tgagctcttc cggcgctcac ccgggatcct
     7141 cgacaccttg caccagcggg cggcccagtg gtacgaggag gcgggcgagg agactgctgg
     7201 gctcgaccat tggctggccg gcaatcgacc tcgagaggcg ctccgcctcc tcgcagccag
     7261 cgaggcgaag ctctacgaca gtggtcgcga aagcacgatc acgcgggtca tcacccggat
     7321 accggtgagc gtgtcggcgg ccgacctcga ggcgcagctg gagttcgcat ggtgccacct
     7381 gctggtcgac aggcatcgct tcctggagta cgtcgcccag gcggttgacg tgagccgcca
     7441 gactcgcggg gtcgacaagg tcctgcgggg cagggtggcg atgttgcagt cgatcgccgc
     7501 gacgatggtc ggcgactggc acgaaggggg tcggcaggcg cgggaagcga tgtctgagct
     7561 gggtgaagcg ggctggctgg acctgctggg ccggttcggc tggaacatgg ttgcccgaga
     7621 catcgcgctg tcggagcgtt ggcacgacgc agccgatgag gtgcgggaag tgcggacggc
     7681 gctcagcgtc gacccagaac gccgattggc cttcgaaggc acgcgagcgg tcggtctcgc
     7741 catggccggc cagccgctcg acgcggtccg cgctgcggcc ggtgttcgaa gctcggcgga
     7801 ggtggcccac ctggcaattc ttcgcggaga gctcgccatc gccgacgcgc tcacccatcg
     7861 cgagctcgga gaccggcagc gtgcgagggc ggagctcacc gccatcgtcg accggccggt
     7921 cgaaccactc acgtacgcgc gcgggctggc ctggctcgag ctgtcgcagc tgtggctgga
     7981 cgagggcgac ctgggcgcgg cacaggctgc gttccgaggt gccgaggcat tcgcggacgc
     8041 cgaacaacac ggcagcgggg ccttggactg gctcgcccga gttggaaccg tggtcgcact
     8101 tgcggctgga gacatgccgg cggcgaaggc ctggtgcgag cgcgttcagg acccgttctg
     8161 gcggccggtg agtctggcgc gcgtccatct ggccgtgggt gacaagccag ccgcagacga
     8221 tgcgttggag agggtcgccg cccggtgtcc gcggcatgaa gtcgtccgtg acctgctccg
     8281 agcgagggca accgcccatc acgaggacgc ggtgaagtat gcgaccgctg cagtcgagct
     8341 ggcttccgcg cacggcatgc tgcagacggt gctctcggag ggtgacgacg tccagcagct
     8401 ggtggagctg gcgtcctgga gagctcccag tgcgtggctg ggccggttcc gccgcggcgt
     8461 gccttccctg gtgcacgctg ctcgacccgt cgtcgcacac ccggacgcgc tcacagatcg
     8521 cgagcgcgac gtgctccggc tgctaccaag tcggctgacc cagcgagaga tcgcggggga
     8581 gctgtccatc tcgctcaata cgctgaagtt ccacctgaag gtgatctacc gaaaactcgg
     8641 agtcaactcg cgaggcgacg ccgcccgatt ggcccgcgac gtactgagcg gtcccagagt
     8701 tcagagcccg aacaccttcg agcgctgaag ctcgaactcc tgcctcgaga cgagacctcg
     8761 atcgacgagc ttggcgagct cccgcacctg ttcgacctgg ttgaccagca cagcggtcga
     8821 ccggcgcgga agggtgcgct cactcagcgg acatcggatc agcaggtcgg ggtaggtgcc
     8881 gcgaggacgg ctcgtccatg ccatcgtctg ctcctgtcag tccttgcccg gtgagacgcg
     8941 ggcaagcgcc gcctccagtc gggactgtgg gatccgctcc ccggcgatca cgttgccgcc
     9001 ggcacgtttc gcagcattcg agaggcggcg cgcccacagg tcctcgacga cgacgatgat
     9061 ggcggcgctg cccggccgta ccaccgtgga tgccaagagc aggtcgcgct cactcaggat
     9121 tccgtccccg atcccgaccg agccggcgag tgccgcgaga ccggggatgt actccggctc
     9181 gatcacctgg acgctgcctt cgagcgtcgt ctcgaccgcg accaggtcaa ggacgcgaag
     9241 agttcgatcc tggacgaggc gtccgagcgg gggtgccagc agggacaggt caccgacctg
     9301 aggcatcgaa acgatcacgt actcgaccag attggtgtcg atcgacatcg actcgccggc
     9361 gttgccggtg acccttgtcc ccgtcatcgc ttcatgacct cgagactcgg cgtgtgccag
     9421 gttcacatca ggacaagatc ttggccttgg cctgttcgaa ctccgcatcg gtgatgatgc
     9481 ccttgtcccg cagcgcggcc aactgcgaga tctcgctcgc gccaccaccg gtgcctgccg
     9541 cggactgcac gtactcgcgc atggccttct cgtgcgcctg tgcatgacgc cgctgatcct
     9601 cgcccatcgc gctgccacgc acgatgaggt acacgagcac gccgatgtag ggcagcacga
     9661 tgacgaagac tgtccacagc gccttccccc atccactgag gtcctcgctg cggaagatgt
     9721 gggcgaagac ggcgatcaac agccagatcc acaggaagaa caggaagaac cacagcatcg
     9781 agaggaaaac gtcgccgact ccgaagtctg cagcaacggt catgtgccac tccttgctca
     9841 cgatctggcc caacagctgc cagcggtgcc ggtctcgaat ccggcctgtg ccggtgttgg
     9901 aacgatcgcg ccggtcgggg tggctgggaa tcaccccgca gcgggtggtt ccgctgcgtt
     9961 cttcgggagt acggcgggat gcgccgccgg actgactggg aagtcagttc gttacctcca
    10021 aaccctcccc ttcggggcgt cgtcgtgaca tcctgcccca cgggtctata gcgtgcagcc
    10081 acacgaggag gcgccccatg cgcaagaagt tgacctttgt ctcattgctg gccttgctag
    10141 tgcttccgct cgccgcgtgc ggcggcgacg acagcggtga caacgacagc tcggcacccc
    10201 ccgccggcag tggtgtgtgc gggtcgaaca agacgaccga cgacctcttg gccgagatct
    10261 gtgacaaggg tgtcatcacc gtgtcgaccg acccgaagta tccgccgcag tcctcgctga
    10321 acgagcagac gggcaagtat gaaggcttcg acatcgacgt ggcgaccgag atcgcgaacc
    10381 ggctcggggt gaaggtcgcc tggcagaccc cggcgtggga ggccatcacg gcgggcagct
    10441 ggaacggccg gtgggacatg agcgtgggat ccatgacacc gaccaacgac cgacagaagg
    10501 tcctcgactt cagccagccg tactacttca cgccggccgt cgtcgtggtg cacaaggaca
    10561 acacgagcgt gcaagacatc gcgaccgacc tggatggcaa gaagatcggc gtctgcagcg
    10621 cgtgcacgta cgagcagttc ctgaagaaga cgctggcgat caagggcttc acgttcgact
    10681 tcgtgatcga cgacgccgag gccgtcgggt acgacactga cacgagtgcg ctgaacgacc
    10741 tggcactcgg agacggcgta cgcctggacg ccgtcatcac ctcgttgaca acggcacagg
    10801 ggttcatcga ctccggcaag ccggtcaaga tcgtcggcga tccggtgttc tacgaaccac
    10861 ttgcggtggc gatcgacaag agcgcgccga aagatccctc cagcctggcc gcggccgtcg
    10921 acaagatcgt cggtgagatg cacgccgacg gcaccctgac cacgatgtcg aagaagtggt
    10981 acgacgggac agacctgacg gtcacgcagt gagccagcgg gctcggccat gagtcgctcg
    11041 gacgacaccc tgctcgacga accgacctcg agatgggaac ggctggcgct caccagcgcc
    11101 ggcatcccgt tccggctcaa ggtggcggcc gtctgggtcg tcatcttcct gatcctggcg
    11161 gggttcttcg ccatcgccga cttcgacacc ggcttcatga aggacgagtt ccccttcatc
    11221 gtgaagggac tcaggtacac gctctacatg gccgttgtcg gcatcgcgct cgcggtcgtc
    11281 ctggcggcac taggagcgct gggtcggatc tcgtccaacc cgatcgcgta cggcgtctcc
    11341 ggcttctacg tctcgttctt cagggggaca ccactgatcg tgcagatgac gctgatctac
    11401 tcggcgctcc cgcagatcgg cttcaacctg gtcgcgaagt atccgagcgt gccggatcag
    11461 cttgccagct tcctgacgtt gcaggccgtg gtcgcgggca tgatcgcgct cggcctcaac
    11521 tacggcgcat acatgaccga gatcttccgg gcgggtatcc agtccgtggc atccggccag
    11581 ggcgaggctg ccgatgcctt ggggatgacc taccggcaga agatgcgcaa ggtcgtgctg
    11641 ccgcaggcct tccgggtgat catccccccg accggcaacg agttcatcgc gatgatgaag
    11701 gacaccgcgc tggtttcttt cctcgggacc gccctcacga gcgctgagat cttccgtcgg
    11761 gcgcagtacg tcggcacgac gcacttccga agcctggaag cccttgtcgt cgccgcaggt
    11821 ctctactggt ccctcaccgt gatcttcacg ttcttccaac gtcggctgga gacccggatc
    11881 ggccgcgggt acgtccgcac cgttggcgtg aaacgacccg gttcggtcgc gcagccccat
    11941 gcccagggag cggggcaggt ggaactgtga gcgagcgtga tgtccgagat gtcatcgtcc
    12001 gggtcgacgg cctgcacaag tacttcggtc acctcgaggt ggtccgcggc gtcgacatgg
    12061 aggtgcggaa gggcgaggtc gtcgtcatct tcggccggtc cggatcgggg aagtcgacgc
    12121 tgctgcgctg tgtcaacttc ctggaggacc cgaccgcggg cacggtcgag gtcgccggca
    12181 tccggctgcg cggcggtcac cgcacccgtc gcaagcggga gcggatccgg cagctgcggc
    12241 tgaacgtcgg gatggtgttc cagcagttca acctgttccc caacatgacc gccctcgaca
    12301 acgtgatgtc aggtccgttg agcgcgaagc gtgggcggga gtcccagatc aaggagcgcg
    12361 cgctcgacct gctcgaccag gtcgggctgt ccgacaaggc gcaggagcac ccgatccggc
    12421 tctccggcgg ccagcagcaa cgcgtcgcga tcgcccgggc gctcgcgatg gactcagagg
    12481 tgatgctctt cgacgagccc acgtcggcac tcgatccgga gctgaccggc gaggtgctcg
    12541 gcgtgatgaa gtcgctcgcc accgagctgc acatgacgat gatggtcgtc acgcatgaga
    12601 tggggttcgc ccgggaggtg gccgaccgga tgtgcttctt ccacgaaggc gtgatcctcg
    12661 aggagggagc ccccgcggac atggtgtcgt cgccacgctt ccccgagacg aagcagttcc
    12721 tcgacgccgt cctgtgaccg ggccgaaacg ccgcgggtcc aaggctctct gcgcgccgcg
    12781 cctgctaccg gtgcattcct cacgcgtcaa gggtcgctgt cgcgatcgct tcgcgacggc
    12841 ttcggtcagg cgcaggtggc ttgttgagcc gcacccggcg actgcgtcgc tcgggtccgt
    12901 ccatcgccct tgactcgtga gcctccatgc accggtttgg ggcagacgat gtcgggcaga
    12961 actgccgtcc tgcccgacgc atcggcgcgg cccgcacctc gccagaatgc caccttcacc
    13021 accaatcgga catgtatcgg tggtggtttg cctcggtagc catccaaacg gtcgggtagc
    13081 caggcagatc tgccttggga ggggtgtggt cgcctgggta gccatgcaaa ccgcacggcg
    13141 acccgaggat gcccagaaat ggtcagtcaa accccaggca aaagggacgg tacggtacgc
    13201 cggccgacca gcctgatgag cagcacggct gtcccgttcg tccatgccga tccagcctca
    13261 gccacgacgg cggtagggcc gccgaccacg caagcaccgc gaccggaccc gccaaaagac
    13321 cggacgagtc ccg
//
LOCUS       DATBFZ010000107         2594 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_63167, whole genome shotgun sequence.
ACCESSION   DATBFZ010000107 DATBFZ010000000
VERSION     DATBFZ010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2594
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63167"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..756)
                     /locus_tag="VH419_03210"
     CDS             complement(58..756)
                     /locus_tag="VH419_03210"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY7042656.1"
                     /translation="MTAPAYVEQQYRTEHRLETRRSVWRDSADGRNPATAAAVAIQAA
                     APRSILEVGCGTGAFAARLVVDNPSADVIATDRSARLVELTAARGVQAQRADVQALPF
                     VDAAFDVVVAMWMLYHVDDLDQGLSEVPRVLRTGGLFVAVTNGDQHLADLLTEAGGSR
                     MLTQFSSENGEAILRRHFTDVSAEHVSTRAVFDDHAAAVGYLASFDQELADHLPFFDG
                     PRDYAGATTVFLAR"
     gene            complement(776..1564)
                     /locus_tag="VH419_03215"
     CDS             complement(776..1564)
                     /locus_tag="VH419_03215"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY7042657.1"
                     /translation="MAGGGIEATFLGHSTVLLRMSGAAVLTDPVLRQSIAVVVRRVAP
                     RIDPASLGQHRLVVISHAHYDHLDTYTLTHLRPGYTLVVPRGVARYVRRVGAAQMIEM
                     SEGDTIDVDRLQVTAVRAIHDGRRHAFGPEAEALGYVIRSDDGSIYHAGDTALYPEMV
                     RLATPRLDLALLPVWGWGPRLGPGHLDPETAAVAASMIKPTAAVPVHWGALWSRVVPR
                     RHHRRTEPPNEFAEAVARISPDVEVSVLEYGQTQRFAATPEGDC"
     gene            1821..2333
                     /locus_tag="VH419_03220"
     CDS             1821..2333
                     /locus_tag="VH419_03220"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEY7042658.1"
                     /translation="MREVFMALLAGDRTHGYELKQSLEQTFGPLLPQLNTGQIYSTLA
                     RLQRDGLIEGESVADDNRGKKLYRLTPTGRQRVEAWIGRPVPGARLKDEFFLKLVVAT
                     SMGLAEPATLIDLQRAEYLQSLRDLDARLAGNGDGPTAELLVEGAVLHLKADLEWLDL
                     IEDRLVRIPT"
     gene            2330..>2594
                     /locus_tag="VH419_03225"
     CDS             2330..>2594
                     /locus_tag="VH419_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008358043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY7042659.1"
                     /translation="MRLLLEARGVVKTYDNADPEIPALAGVDMSLDHGEFVALVGPSG
                     CGKSTLLNVLAGLDRPSAGEVWFDGERTDQLGETALAKLRRKRI"
BASE COUNT          405 a          839 c          927 g          423 t
ORIGIN      
        1 cgcccggccg ctgatcgagc ttgtcgagat caaggtcggc cccgccgtgg gcaccggtca
       61 gcgcgcgagg aacacggtgg tggcaccggc gtagtcgcgc ggaccgtcga agaaggggag
      121 gtggtctgcc agctcctggt cgaagctggc caggtagccg acggcggccg cgtggtcgtc
      181 gaagacggcg cgggtgctga cgtgctctgc cgaaacgtcg gtgaagtggc gtcggaggat
      241 ggcttcgccg ttctcgctgc tgaactgcgt gagcatgcga ctcccgccgg cctcggtgag
      301 gaggtcggcg aggtgctggt cgccgttggt gaccgcgacg aagagtccac ccgtccggag
      361 tacgcggggt acctccgaca gcccttggtc gaggtcgtcg acgtggtaca gcatccacat
      421 cgcgacgacc acgtcgaacg cagcatcgac gaacggcagt gcctggacgt ctgcccgctg
      481 tgcctgcacg ccgcgcgcgg cggtgagctc gaccaaccgc gcggaccggt ctgtggcgat
      541 cacgtcggcc gatgggttat caacgacgag ccgggcggcg aacgcgcccg tcccgcagcc
      601 gacctcgagg atcgagcgag gcgctgccgc ctggatagcg actgccgccg ctgttgccgg
      661 gttgcgcccg tcggcggagt cgcgccacac cgagcggcga gtctccagtc ggtgctcggt
      721 ccggtactgc tgctcgacgt acgcgggtgc cgtcatctcc tccacggcgc gcaggttagc
      781 aatcgccctc cggggtggcg gcgaaccgct gtgtctggcc gtactcgagc acgctgactt
      841 cgacgtccgg gctgatccgg gcgacggcct cggcgaactc gttcgggggc tcggtgcgac
      901 ggtggtgccg gcggggaacg accctggacc acagcgcacc ccagtgcact gggacggccg
      961 ctgtgggctt gatcatcgac gcggcgaccg ctgcggtctc ggggtcgagg tgtcctggac
     1021 cgagtcgcgg accccagccc cagaccggca gcaacgcgag gtcgaggcgc ggcgtcgcga
     1081 ggcgcaccat ctcggggtac agcgcggtgt cgccggcgtg gtagatggag ccgtcgtcgg
     1141 acctgatgac gtagccgagc gcctcggcct cgggaccgaa cgcgtggcgg cgtccgtcgt
     1201 gaatggcccg caccgccgtc acctgtagtc ggtcgacatc gatcgtgtcg ccctcggaca
     1261 tctcgatcat ctgcgcggca ccgactcggc gcacgtagcg cgcgacgccg cggggcacga
     1321 caagcgtgta tcccggccgc aggtgcgtca acgtgtaggt gtccaggtgg tcgtagtgcg
     1381 cgtggctgat caccacgagc cggtgctgac cgaggctcgc cgggtcgatc cgaggagcga
     1441 ctcggcgtac gacgacggcg atggactgcc tgagcacggg atctgtgagg actgcggcac
     1501 cagacatccg caacagcacc gtggagtgcc cgaggaacgt cgcctcgatc ccaccgcccg
     1561 ccatgcgccg ttcatacgcc gaggtggcaa gaatcgtcca attctgtcgg cgtgtcggac
     1621 gctctgcgcc acctcgatcg agcgctcgat cggcagacct tcaggcgggc ggcatgaacg
     1681 tgcgtgctgt tcggcagcgg cgtactggtc gatctgccgc cgatctcgtc ctgttgtccc
     1741 tgcggggaag cggttgcgcc ctgcagccag gttctcgcgg cccttccccg atggatactc
     1801 gaaatgtata ctcgctatct atgcgagaag tcttcatggc gttgctggcg ggcgaccgta
     1861 cccacggata cgagctcaag cagtcgctgg aacagacgtt cgggccgttg ctcccccagc
     1921 tcaacaccgg gcagatctac tccacgttgg ctcggctcca gcgtgacggg ctcatcgagg
     1981 gggagagtgt ggcggacgac aaccgcggca agaagctgta tcgcctcact ccaaccggtc
     2041 gccagcgggt cgaggcgtgg atcggccggc cggtcccggg agcacggctc aaggacgagt
     2101 tcttcctcaa gctcgtggtc gccacgtcga tggggcttgc cgagcccgcg accttgatcg
     2161 acctgcagcg tgctgaatat ctccagtcgt tgcgcgacct cgacgcacga ctggcaggca
     2221 acggcgacgg accgaccgcg gaactgctgg tggaaggtgc ggtcttgcac ctgaaagcgg
     2281 acctcgagtg gctggatctc atcgaggatc ggctcgtgag gataccgacg tgaggctcct
     2341 tctcgaggcc cgtggtgtgg tcaagacata cgacaacgca gatcctgaga tcccggcgct
     2401 cgcgggcgtc gacatgtccc tcgaccacgg tgagttcgtg gcgctggtcg gtccaagcgg
     2461 gtgcggcaag tcgacgctgc tgaatgttct cgcggggctc gaccgcccga gcgcgggcga
     2521 ggtgtggttc gacggcgagc gcaccgacca gttgggcgag accgcgctcg ccaagctccg
     2581 gaggaagcgc atcg
//
LOCUS       DATBFZ010000108        17238 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_4003028, whole genome shotgun sequence.
ACCESSION   DATBFZ010000108 DATBFZ010000000
VERSION     DATBFZ010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 17238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17238
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4003028"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..734)
                     /locus_tag="VH419_03230"
     CDS             complement(<1..734)
                     /locus_tag="VH419_03230"
                     /inference="COORDINATES: protein motif:HMM:NF015083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase family protein"
                     /protein_id="HEY7042660.1"
                     /translation="MVDPTDAQNAFIKAVDAVDHERGWDSLPVPLGLVALIGLRDELR
                     QKNLYDTYEGDPPEAPKPGPTHLTARTVDGSYNDLSVPSMGMAQTRFGRNVPLDRGFA
                     EVPPQLFDPNPRTVSNELLLRTEFKPASTLNVLAAAWLQFQIRDWFSHGSDPSRMVEV
                     PRPEGDDWDDDAIRIPSTPADPTATPDQQLTTFLNTETHWWDASQMYGSTPEFQQAVR
                     TGEAGKVRIGADGLIDLDPSLLGKSGG"
     gene            complement(796..1170)
                     /locus_tag="VH419_03235"
     CDS             complement(796..1170)
                     /locus_tag="VH419_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042661.1"
                     /translation="MLSRDAARKTLGAIRFVNGAAGLFVPTLLLRRLGVDSTTSQPAT
                     YPFRMFGIRTMIIGADLWLLRGEELRRATKVAVLIHATDTVSAAATAVRGDLPRKQAL
                     VATGISAVNTALAIAAARPGQP"
     gene            1347..1847
                     /locus_tag="VH419_03240"
     CDS             1347..1847
                     /locus_tag="VH419_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042662.1"
                     /translation="MSSTSADVTEQTGIDENVLLEMSNTDLDELFRRSDAGPIPAGNM
                     RGTVLAFPGSMLAKPLAAVVYLVGWQGKVVDRQRGILLNKITPLRLRLIAAKVSHDTS
                     WVDSGECVLLDYSHTSLVAKMVRDEIRLVAPSLYLGVVWLWKRRVAWFTLRSPGDSAA
                     SDPGAW"
     gene            1860..3140
                     /locus_tag="VH419_03245"
     CDS             1860..3140
                     /locus_tag="VH419_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042663.1"
                     /translation="MPGQQPVTLRAPVVPGRLDALRGVLESGAISGGRPGAAPPPLRL
                     AQLPGVHFARLFVLEAMTLQTGEPVPASLVYMADVDGSVDRHLRELVHRAPGGVDAVF
                     GHCVGYPTAPTVLERLTWLRSHALRPAAYYVHTAGRTLTQIKDEALLYSTLGQIVDTG
                     EVVTAGADPVQLRSRLRDAVLRRAGFGWIRRPPPGPGLVQELRDAANLLLTLAVLIVA
                     IPFVVVPLGVWLVLIRISELRDTQDSGVPDQAHVDEVRRYEDFGAQNPFTAVGEVKSG
                     VVRRLTMRVALAGLGFACRHAFARDNLAGVTSIHFARWVPIDDGQRLIFASSYDGSLE
                     SYMDDFISRLSWGINLVFSNGVGFPRARWLVLDGSRDEISYKHYLRRHQVPTVVFYSG
                     YPNYSAANLDTFTAVHSGIGRQDETSAQTWLAQL"
     gene            3169..4590
                     /locus_tag="VH419_03250"
     CDS             3169..4590
                     /locus_tag="VH419_03250"
                     /inference="COORDINATES: protein motif:HMM:TIGR01413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HEY7042664.1"
                     /translation="MADALELDDIQGLVARGYGRLPHAAYLLAHVEEPTAAGELLRGW
                     AEDGTVTPASESRPSRAMNIAVTADGIRTLTGTTELDDGFAEPFLTGMATAYRSRLLG
                     DVGANDPAGWQWGGPGTDPVHVLVLVYAADAEALATWAADLVSALAGHGMRLLLTLPA
                     AELTAREPFGFRDGISQPLIAGLGSAQREGDVVRDGEFVLGYVNEYSQRTERPLLPTS
                     ADPDRLLPRDPDGSGSADLGRNGSYLVFRQLQQDIDGFENYLSRAATVDGHVDKTMRE
                     VLAAKLVGRWRGSGAPLVLAPEYDDQALEAANDFGYHQLDPQGLRCPVGAHIRRANPR
                     DVLPPNPGTQESRAVNHRHRLLRRGRAYTTTDETGAATEHGLFFLCLNTNPARQYEFV
                     QHSWVNDPSFNGLVGVEDPLVGPRTNGPGRFDVPSEPVRRRFEGLPQFVTVRGGGYFF
                     LPGIRALRFLTSTPPPQRREEAR"
     gene            4592..5839
                     /locus_tag="VH419_03255"
     CDS             4592..5839
                     /locus_tag="VH419_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase family protein"
                     /protein_id="HEY7042665.1"
                     /translation="MDPQGKLTFVVFRPLSDLFLWVWRLERRFEFLYRPQFDRWLRPP
                     LFAAAQWLQNRRRPDEGLALVEEKLLPDEEAHIQTIIDELKAFTRENWLPGSAQRFGN
                     TKTFGVLRAEFTVLPSLPEHLRQGLFAEPKSYPAWVRFSGPGPYAPPDLEDFGQCSVA
                     IKVMGVAGEKLLDDEQHTQDLILVSPASFVTPNVTENAKMQRWVRAKAPIVYAIMPGD
                     SHLLHLVMQLLYSPMHANPLEVQYYSNVPFLLGDDQAVEYSLKPTQPGDSKIPVRPTE
                     NYLRDAMVRTLSRGDWSMDFMVQVQTDAHLMPIEDATVKWPERLSPYLPVARLRLLRQ
                     RFDSDEQLAFADVLRYNPWHSRREHKPLGNSNRARRRMYAELAELRQQMNDVTHVEPT
                     GDESFPDNPAPASTTTTTTPTPG"
     gene            complement(5923..7245)
                     /locus_tag="VH419_03260"
     CDS             complement(5923..7245)
                     /locus_tag="VH419_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HEY7042666.1"
                     /translation="MAVRRDPVSVSLSTADARRIALLAQGFGEPRPPGRVDVRHLRRT
                     LDRLGVLQIDSVNVLCRSHYLPLFARLGPYPRERLDAMAWGGGRRELFEYWGHRASLL
                     PVSLHPLMRWRMDAARVQHWDFELLGWRTAVDPAVAAPWAVIAGMTRLSRESPGLVDE
                     VLAVVAERGPIAAGEVDRERRSRDAPDPDPTTGAMWNWQDAKIALELLFSAGQVTTAT
                     RRGFERAYDLTERVLPTEVLAVPTPDRADAQRELVRIAARAQGVATERHLRDYFDLPA
                     AEAKVRIAELVDTGELLAAQVEGVSQRSFLWATAEARRPVTGQALLSPFDSLIWDRDR
                     TERLFGFHYRISIYTPEAQRTHGYYVLPFLLGERLVARVDLKSDRRASTLLVQGAYAE
                     PGVSTPEVAAALGDELRAMADWLELDRVHIVKRGDLATTLAGRLPSQP"
     gene            7296..8549
                     /locus_tag="VH419_03265"
     CDS             7296..8549
                     /locus_tag="VH419_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014847483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEY7042667.1"
                     /translation="MNLNRGQATEPTTLRLARELGPELAELRRALHQIPEVGLDLPLT
                     QRMLLDALAGLDLEVSLGKELSSITAVLRGRAPAEGERQVVLLRGDMDALPVTEEVDL
                     DYRSRHDGVMHACGHDLHMAGLVGAARILHALRDELPGDVVFMFQPGEEGPGGAEPMI
                     REGLLDVAGRRVDAAYALHVSSSDEPFGQWFSRRGTSGASADSVTVRVLGTGGHGSAP
                     YRARDPIPAMCEMVLALQTMTTRGFDVFDPIVVTVGRIAGGTKENIIPDEAEFEATVR
                     SLSPQSRAKVRADIQRLVGGIAAAHGLAVAVDYELGYPVTVNDDTEYAHALQVVDDLF
                     GRDRFTELPTPELGSEDMAFVMELVPSAYFNVSACPVADYANAPDNHSPRAAFDDAVL
                     PDAAAWLAEVAVRRLRRATPGPDSA"
     gene            8622..9095
                     /locus_tag="VH419_03270"
     CDS             8622..9095
                     /locus_tag="VH419_03270"
                     /inference="COORDINATES: protein motif:HMM:NF020908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HEY7042668.1"
                     /translation="MGFESWIDRQIREAEERGEFDNLPGAGKPLKDLDKPQDADWWVK
                     RLIARENLPPVLPTALALRKEVEDLQETLRPLWRESDVRHVVDDLNQRIIDARRRPSA
                     GPAVFVKTVAVDEAVERWRADRERRLGGGARRSEPADGGQRHDRGRRRWFGRRRS"
     gene            9159..10991
                     /gene="malQ"
                     /locus_tag="VH419_03275"
     CDS             9159..10991
                     /gene="malQ"
                     /locus_tag="VH419_03275"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEY7042669.1"
                     /translation="MNPDDGRPRAAHDERLEPDTWGIHRCWMNSADELVEVPGRTIER
                     LRTIIGRPPDDLAERAPLVTQPGRDLRLGVLDVECEDGQRRTIDGIVPDDFPLGYHDT
                     TVGGIRRRLIVSPGSCWLPPGRHGWGWAVQLYAARSRDSGGIGDLADLRTIRVWAQGL
                     GASFLLVNPLHAVAPTFPQEPSPYLPASRRFRNPLYLRVAHVAHTNPVDELIDRDAVW
                     RAKRTALEAEFATRSPGAAFHRWRAEQGMALEAFATWCVLAEHHGPDWQAWPTGLHDP
                     NGAGVRGFAREHAERVTFHAWLQWRLDEQFTAAAGETRIVQDLPVGVDGGGADAWMWQ
                     ETVAAGATVGAPPDVFNAQGQGWGSPPMAPWRLRLANYEPFVESIRATMSGGGGIRID
                     HVMGLFRLWWIPEGLPPSDGAYVRYPSQDLLDIVALESHRAQAVVVGEDLGTVEPGVR
                     ETLQERAVLSYRLLWFEDAPPEDWPELAMAAITTHDLPTVAGLWTGADLEDQVANLST
                     PRPALERDRAALLSRLPSMPEGADGVALAAHRTLAGASSMMISATLEDAVGQQLRPNM
                     PGTRTRPNWSIPLPVRVDDLPNHAQTSAIAKILQRGLPAPSGRD"
     gene            11024..11656
                     /locus_tag="VH419_03280"
     CDS             11024..11656
                     /locus_tag="VH419_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020390724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HEY7042670.1"
                     /translation="MGTHFQGSLLDSPELAAPSLGDLGGLRRTRLARGAWVDVLPTWV
                     HGSDGLFERLIETVPWYAERRQMYERVVDVPRLLCFYGEDADLPDPLLDEARRVLSAH
                     YADELGEPFTTAGLCYYRDGRDSVAWHGDTIGRSRTQDTMVAIVSFGSPRRLSLRPSG
                     GGSSIGFSLGHGDLIVMGGSCQRTWEHAILKTARPAGPRISVQFRPHNVR"
     gene            complement(11747..12961)
                     /locus_tag="VH419_03285"
     CDS             complement(11747..12961)
                     /locus_tag="VH419_03285"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020580251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEY7042671.1"
                     /translation="MVFSALAHLECPRCSTQYDASVLQGLCRADGSPLLARYDLSAVD
                     VRRDDVSGRPPDLWRYHELLPVSAPEHVVTLGEGMTALLEAPRLAADLGLHRVLIKDD
                     GLLPTGTFKARGAAVGVSRAKELGARRLAMPTNGNAGAAWSAYAARAGLIATIAMPVD
                     APTITRAETVATGGDLRLVDGLISDAGRLVADGIATSTEEWFEVATLKEPYRIEGKKT
                     MGLEIVEQFGWRMPDVIVYPTGGGVGLIGIFKALTEIRELGWVDGPLPRLVCVQSTGC
                     SPIVDAFVAGADESVMAEDSSTVAFGINVPKALGDFLVLDAIRETGGTAVAVDDQDLL
                     TDLGRVGRLEGLFMCPEGAATVSAVRALASSGWVSPDDEVVLLNTGAGVIYPGTVQVE
                     APTIPKHGKLEL"
     gene            13130..13753
                     /locus_tag="VH419_03290"
     CDS             13130..13753
                     /locus_tag="VH419_03290"
                     /inference="COORDINATES: protein motif:HMM:NF019533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HEY7042672.1"
                     /translation="MSAVDVWDWRRRTFVLYAEVRAHTDTAAAHRHWIEQRDVMLRSH
                     PASPVPARAQSSYPGAEVAAYDAAHRFVVRIDRDVEPETIEYQTGTDGVVPFDRIGRV
                     HLPGLGSLDVWWLASYGGGLFLPFRDRASTSYGGGRYLLDTVKGADLGGDADALVVDL
                     NFAYQPSCAYDEAWACPLAGPTNTLDVEVPVGERHVRLSDDTDGSTR"
     gene            13750..14307
                     /locus_tag="VH419_03295"
     CDS             13750..14307
                     /locus_tag="VH419_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016460836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEY7042673.1"
                     /translation="MTIPTIGTLEPLPAAERPDLLADPVRKALAGWERAGGVGVFEID
                     PELADTQALTDAYDLPLAASANCVVVAGRRGGDERIAACVVTADRRADVNNLVRRMLD
                     VRKASFLSTDRAVEETSMEYGGITPIGVPARWRLLVDASVARLDVAIIGSGVRRSKLL
                     LSGADLAAYPGAELVDGLGLPVQPR"
     gene            14363..14917
                     /locus_tag="VH419_03300"
     CDS             14363..14917
                     /locus_tag="VH419_03300"
                     /inference="COORDINATES: protein motif:HMM:TIGR03086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03086 family metal-binding protein"
                     /protein_id="HEY7042674.1"
                     /translation="MSEVSSRFARLAATMTDRISAVDDGAWENPSPCQGWTARDLVQH
                     LVDTYGMFGGFVDQQLTPGSEVAEDPLGAWISARDQMQAWLDDPAVAKREVQGVSGTT
                     TFEASVNRFICFDLLVHGWDLAKATGQDTTIDPAELSNLWADTEAFGDNIRTGGVCGP
                     ELPAPEDATEQERLLAFLGRDARA"
     gene            14993..16243
                     /locus_tag="VH419_03305"
     CDS             14993..16243
                     /locus_tag="VH419_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012923823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HEY7042675.1"
                     /translation="MSALLEVTTADVVRRRSLRRMRTVATCLLLFAAVVYVATLHRSG
                     GWAYLHATAEAAMVGAIADWFAVTALFRHPLGIPIPHTALIPTRKNMLARSLQDFVTQ
                     NFLQESVVRTRVSDAQVSHRVGEWLRQPGHSARVVSEAAALIEIGLARIRDEDVDYFV
                     RGELLPRLSEERLAPLAGQLLGDVVAEGSHHGLVDLVLAEADRWLATNEAAFAGAVLT
                     RAPWWSPTWLDEKVVARLHREVLAWVREIRDDPSHHARRALDDLLSRLAKDLCEDVDT
                     QARAERLKQRILAQPQVADTALSLWKALSRTLQTSVRDPEGALRQRAVHRLQTFGEQL
                     VSDTALQARLDEHAADLAAFVVGRYGDELATVITDTVERWDGREAASRIELYVGRDLQ
                     FIRINGTLVGGLAGLVIYSVAELL"
     gene            complement(16318..16833)
                     /locus_tag="VH419_03310"
     CDS             complement(16318..16833)
                     /locus_tag="VH419_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017936804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY7042676.1"
                     /translation="MKSRLDALEVRERTLDHPDALALISDVQEYYVLIYGGPDTSPID
                     PRELARPDGAFFIGYLAGEPVVMGGWRFAIEALDVPAQRPAELKRMYVVPAHRGTGLG
                     KTMLRHLEQSALAAGADMLVLETGSPQVDAVGLYLGSGYSEIPRFGHYKDEPAAVHLG
                     KSLSGTAPSAP"
BASE COUNT         2665 a         5906 c         5983 g         2684 t
ORIGIN      
        1 ccgccggact tgcccagcag cgacgggtcg aggtcgatca gaccgtcggc accgatccgc
       61 accttgcccg cctcgccggt ccgaacggcc tgctggaact ccggggtact cccgtacatc
      121 tgtgacgcgt cccaccagtg ggtctcggtg ttgaggaacg tcgtgagttg ctggtctggt
      181 gtggccgtcg ggtcggcggg cgtggacggg atgcggatcg cgtcgtcgtc ccagtcgtcg
      241 ccctcgggtc gcgggacctc gaccatccgg gacgggtcac tgccgtggct gaaccagtcg
      301 cggatctgga actgcaacca ggccgcggcg aggacattca gggtcgaggc aggtttgaac
      361 tcggtccgga gcaacagctc gttgctgacc gttcgcgggt tggggtcgaa cagctgcggc
      421 ggcacctcgg cgaaacccct gtcgagcggc acgttgcgcc cgaacctcgt ctgcgccatc
      481 cccatggacg ggaccgaaag gtcgttgtac gagccgtcca cggtacgcgc ggtgaggtgc
      541 gtcggacccg gcttcggagc ctcgggcgga tcgccctcat acgtgtcgta caggttcttc
      601 tggcgcagct cgtcgcgaag cccgatcagt gcgacgaggc cgagcggcac cggcagggag
      661 tcccatccgc gctcgtggtc gacggcgtcg acggctttga tgaacgcgtt ctgggcatct
      721 gtcgggtcga ccatcgcgga tctccttctg acggcgtacg gcggacgagg tctggcgtgg
      781 cgggtgccga cacggtcacg gctgtccggg acgcgcagct gcgatcgcca gggcggtgtt
      841 gacggcggag atcccggtgg cgaccagcgc ctgctttcgt ggcaggtcgc cgcggaccgc
      901 ggtggctgcc gccgacaccg tgtcggtggc gtggatcagc acggcaacct tcgtcgcgcg
      961 gcgcagctcc tcaccgcgca acagccacag atccgccccg atgatcatcg tgcggatgcc
     1021 gaacatccgg aacgggtagg tggctggctg actcgtcgta ctgtcgacac cgagccgtcg
     1081 cagcaacagc gtcggcacga agaggccggc cgccccgttg acgaagcgga tcgcacccaa
     1141 ggtcttgcgc gcggcgtcgc ggctcagcat cgcccatccc ccatcgctcg agtgtttcga
     1201 ggattgcccg cttttggaga ggtgtcaacg aaggctcgtc cggtccgcgg ttcgtcacgt
     1261 aaccctttac acccgacccc aggacgagca gtctgaggag acgcggcgct ctttgtcgcg
     1321 ggctgccagc ggggagggga acacgcatga gcagtacgtc agcggatgtc accgagcaga
     1381 ccggcatcga cgagaacgtc ctgctggaga tgagcaacac ggacctggac gagctcttcc
     1441 gacggtcgga cgcgggcccg atcccggccg gaaacatgcg cggaaccgtg ctggcgttcc
     1501 ccgggtcgat gctcgccaag ccgctcgccg ccgtcgtgta cctggtgggc tggcagggga
     1561 aggtcgtcga ccggcagcgc ggcatactgt tgaacaagat caccccgctc cggctgcgcc
     1621 tcatcgccgc caaggtcagc cacgacacga gctgggtgga cagcggcgag tgcgtcctgc
     1681 tcgactactc gcacacgtcg ctggtggcga aaatggtgcg cgacgagatc cgactcgttg
     1741 ccccgtcgct gtatctcggc gtggtgtggt tgtggaagcg acgggtggcg tggttcacgc
     1801 tgcgctcgcc cggcgacagc gccgcgtccg accccggcgc gtggtgagcg caacctgaca
     1861 tgccgggcca gcagccggtg acgctgcgag cgccggtcgt acccgggcgc ctggatgccc
     1921 tgcgcggtgt gctcgagtcc ggcgcgatct cgggtgggcg acctggtgcc gcgccgccac
     1981 cacttcggct cgctcagctc ccgggtgtcc atttcgcccg gctgttcgtg ctcgaggcga
     2041 tgacgttgca gacaggggag ccggtccccg cgtcgctggt gtacatggcc gacgtcgacg
     2101 gttccgtcga ccgccatctg cgtgagctcg tgcatcgtgc cccaggcggc gtcgatgcgg
     2161 tgttcggcca ctgtgtcggg tatccgaccg cgccgaccgt gctggagcgc ctgacctggt
     2221 tgcggtcgca cgcgctgcgt ccggcggcgt actacgtgca cacagcgggc cggacactga
     2281 cacagatcaa ggacgaggcg ctgctctaca gcacgctggg tcagatcgtg gacaccggcg
     2341 aggtcgtcac ggcaggtgct gatcccgtgc agctgcgcag ccggttgcgt gatgctgtgt
     2401 tgcgccgggc cggtttcggc tggatcaggc ggccaccacc cggcccgggg ctcgtgcagg
     2461 agctgcgcga cgccgcgaac ctgctgctca ccctcgctgt gctgatcgtc gcgatcccgt
     2521 tcgtggtcgt tccgctcggg gtctggctcg tgctgatccg catcagcgag cttcgtgaca
     2581 cccaagacag cggcgtcccc gaccaggcgc acgtcgacga ggtacgccgg tacgaggact
     2641 tcggcgcgca gaacccgttc accgcggtcg gcgaggtcaa atccggcgtc gtacgtcggc
     2701 tgacgatgcg ggtcgcgctg gccggactgg gtttcgcctg ccggcatgcg ttcgcccgcg
     2761 acaacctcgc cggtgtcacg tcgatccact tcgcccggtg ggtgccgatc gacgacgggc
     2821 agcggctgat cttcgccagc agctacgacg gcagcctcga gagctacatg gacgacttca
     2881 tctcccggct gtcgtggggg atcaacctgg tgttcagcaa cggcgtgggg ttcccgcgcg
     2941 cccggtggct ggtgctcgac ggctcgcggg acgagatctc ctacaagcac tacctgcgcc
     3001 gtcaccaagt gccgaccgtg gtcttctact ccggctaccc gaactactcc gcagcgaacc
     3061 tcgacacgtt cactgcggtg cattcaggca tcggccggca ggacgagacg agcgcgcaga
     3121 cctggctggc gcagctgtga gaaagacctg agcgcatgga gtgccggcat ggctgacgcc
     3181 ctcgaactcg acgacatcca aggtctggtc gcccgtgggt acggccgact gccgcatgcc
     3241 gcatacctgc tcgcccacgt cgaggaaccg accgcggcgg gcgaactgct gcgcggctgg
     3301 gccgaggacg ggaccgtcac gccggcatcc gagtcgcgtc cgtcgcgggc catgaacatc
     3361 gcggtgaccg ccgacgggat ccggacgctc accgggacga ccgagctgga cgacgggttc
     3421 gccgaaccgt tcctgaccgg gatggcaacc gcgtatcgca gccgtctgct gggggacgtc
     3481 ggcgcgaacg acccggccgg atggcaatgg ggtgggcctg gaacagatcc ggttcatgtg
     3541 ctggtgctgg tgtatgccgc ggacgccgaa gcactggcga cctgggcagc cgacctcgtc
     3601 tcggcgctgg ccgggcatgg catgcggttg ctgctgacgc tgccggcggc agagctgacc
     3661 gctcgtgaac cgttcgggtt ccgcgacggc atctcccagc cactgatcgc cggcctcggc
     3721 tcggcgcagc gagagggcga cgtcgtccgg gacggcgagt tcgtgctcgg ctacgtcaac
     3781 gagtacagcc agcgcaccga acgcccgctg cttccgacgt ccgccgaccc ggaccggctg
     3841 ttgccgcgag accccgacgg atccggctca gcggacctgg gtcggaacgg cagctacctg
     3901 gtcttccggc agttgcagca ggacatcgac ggcttcgaga actacctgag ccgcgccgcg
     3961 accgtggacg gccatgtcga caagaccatg cgcgaagtcc tggccgccaa gctggtgggc
     4021 cgatggcggg ggtctggtgc gccgctggtc cttgcgccgg agtacgacga ccaggcgctc
     4081 gaggctgcca acgacttcgg ctaccaccag ctcgacccac agggtctgcg gtgtccggtc
     4141 ggtgcgcaca tccgtcgggc caacccgcgg gacgtgctgc cgcccaaccc cggaacccag
     4201 gagtcgaggg ccgtgaacca tcggcaccgc ctgctgcgtc gtggacgcgc gtacacgacg
     4261 acggacgaga cgggagcggc caccgagcac gggctcttct tcctgtgcct gaacacgaac
     4321 ccggcgcggc agtacgagtt cgtgcagcac agctgggtga acgacccgag cttcaacggg
     4381 ctggtcggtg tcgaggatcc gttggtcggt ccgcgcacga acgggcccgg ccggttcgac
     4441 gtaccgtccg aaccggtgcg gcgtcgtttc gaaggactgc cgcagttcgt cacggtccgc
     4501 ggcggcggct acttcttcct gcccgggatc cgggcactgc gcttcctgac ctcgacgcca
     4561 ccaccacaac gacgcgagga ggctcgctga catggacccg caggggaagc tgacgttcgt
     4621 cgtgttccgg ccgctgagcg atctcttctt gtgggtctgg cggttggaac gacggttcga
     4681 gttcctctac cggccgcagt tcgaccggtg gctgcgaccg ccgctgttcg cggcggcgca
     4741 atggctgcag aaccggcgtc gacccgacga aggcctggcc ctggtcgagg agaagctgct
     4801 gcccgacgaa gaggcgcaca tccaaacgat catcgacgag ctgaaggcct tcacccgcga
     4861 gaactggctg cctggttcgg cgcagcgctt cggcaacacg aagacgttcg gcgtactccg
     4921 tgccgagttc accgtgctgc cgagcctccc cgagcacctt cgccagggcc tgttcgccga
     4981 gccgaagtcg tatcccgcgt gggtccggtt ctccggcccg ggaccgtacg cgccacccga
     5041 cctggaggac ttcggccagt gttcggtcgc gatcaaggtg atgggggtgg ccggggagaa
     5101 gctgctggac gacgaacaac acacgcagga cctgatcttg gtgagtccgg caagcttcgt
     5161 gaccccgaat gtcacggaga acgccaagat gcagcgctgg gtccgagcga aggccccgat
     5221 tgtctacgcg atcatgcccg gcgactcgca cctgctgcac ctggtcatgc agctgctgta
     5281 ctcaccgatg cacgccaacc cgttggaagt gcagtactac agcaacgtcc cgttcctgct
     5341 cggcgacgac caggcggtcg aatactcgct caagccgacc cagcccgggg acagcaagat
     5401 cccggtgcgg ccgacggaga actacctgcg cgacgccatg gtccgtacgc tgtcgcgcgg
     5461 cgactggtcg atggacttca tggtgcaggt gcagaccgat gctcacctca tgccgatcga
     5521 ggacgcgacg gtcaagtggc cggagcggtt gtcgccgtac cttcccgtcg ccagactgcg
     5581 gctgctgcgg caacgtttcg actccgacga acagctcgcg ttcgccgacg tgctgcgcta
     5641 caacccgtgg cacagccggc gcgagcacaa gccgctgggg aactcgaacc gggcccgtcg
     5701 acggatgtac gccgagctcg ccgagctccg gcagcagatg aacgacgtga cccacgtcga
     5761 gcccaccggc gacgagagct ttcccgacaa ccccgccccc gcgagtacca ccacgaccac
     5821 cacccctacc cccggctgac gctgaccgcg aattgcggga aagttgctgc cgaaacgccg
     5881 cttcagacgt gcactttccc gcaattcgcg gggaccccgg gctcagggtt ggctgggcag
     5941 gcggccggcg agggtggttg cgaggtcgcc gcgcttgacg atgtgcacgc gatcgagctc
     6001 gagccagtcg gccatcgcac gcagttcgtc gccaagtgct gctgccacct ccggcgtgga
     6061 gacgcccggt tcggcgtacg cgccctgaac cagcagcgtc gaagcacgcc ggtccgactt
     6121 caggtcgacg cgggcgacga gccgctcacc cagcaggaaa gggagcacgt agtagccgtg
     6181 cgtgcgctga gcctctggtg tgtagatgct gatccggtag tggaagccga acagccgttc
     6241 ggtgcggtcg cggtcccaga tcagcgagtc gaagggcgac agcaaggctt gaccggtgac
     6301 cggacgccgt gcctccgccg tcgcccagag gaagctccgt tgcgacacac cttcgacctg
     6361 ggccgcgagc agctctccgg tgtcgaccag ctccgcgatc cgcaccttcg cctccgcggc
     6421 cggcaagtcg aagtagtcgc gcaggtgtcg ctcggtggcc accccttgcg cccgggccgc
     6481 gatccgcacc agctcccgct gcgcgtccgc tcggtccggc gtcggcaccg ccagcacctc
     6541 cgtggggagc acccgctcgg tgaggtcgta cgcccgttcg aagccccgac gcgtcgcggt
     6601 ggtcacctgg ccggcggaga acaacagctc cagcgcgatc ttggcgtcct gccagttcca
     6661 catcgccccg gtcgtggggt cggggtccgg cgcgtcgcgt gaccggcgct cgcgatcgac
     6721 ctcgccggcc gcgatcggcc ctcgctccgc gacgaccgcg agcacctcgt ccaccagccc
     6781 cggactttcg cgggacagcc gggtcatgcc ggcaatcacc gcccacggcg cagccactgc
     6841 cgggtccacg gcggtccgcc agccgagcag ctcgaagtcc cagtgctgga cccgggccgc
     6901 gtccatccgc cagcgcatca acggatggag ggagaccggg agcaacgatg ctcggtgccc
     6961 ccagtactcg aagagctccc gtcggccgcc accccacgcc atcgcgtcca gccgttcccg
     7021 cgggtacggg ccgagccgcg cgaacaacgg caggtagtgc gaccggcaaa ggacgttcac
     7081 cgagtcgatc tgcagtacgc cgagccggtc cagcgtgcgg cgcaggtggc ggacatccac
     7141 ccgtcccggc ggtctcggct caccgaagcc ctgtgcgagc agggcgatcc gccgcgcgtc
     7201 ggcggtcgac aacgacaccg agaccgggtc tcgtcgcacc gccatactcc gacgctatcc
     7261 agtcggtgac gaccgggcgg cctagactcc ggcgcgtgaa cctcaaccgt ggacaggcga
     7321 ccgagccgac gacgctccga ctggcacgcg aactcggccc cgagctggcc gagttgcggc
     7381 gagcgctgca ccagataccc gaggtcggcc tcgacctccc gttgacccag cgcatgctgc
     7441 tcgacgcgct ggcgggcctc gacctggaag tctcgttggg caaggagctc tcgtcgatca
     7501 ccgcggtgct ccgtggccgg gctcccgccg agggtgagcg acaggtcgtc ctgcttcgcg
     7561 gcgacatgga cgccctcccg gtgaccgagg aagtcgacct cgactaccgg tctcgtcacg
     7621 acggcgtcat gcatgcgtgc gggcacgacc tccacatggc cggcctggtg ggtgcggcgc
     7681 ggatcctgca cgccctgcgt gacgagctcc ccggcgacgt cgtcttcatg ttccagcccg
     7741 gcgaggaagg tcccggcggc gctgagccga tgatccgcga gggactgttg gacgtggccg
     7801 gccgccgggt cgatgcggcg tacgcgctgc acgtcagctc cagcgacgaa cccttcggcc
     7861 agtggttctc acgtcgtggc acgagcggag cctcggcgga ctcggtcacc gtccgggtgc
     7921 tggggacggg aggtcatggg tccgcgccgt accgcgcaag ggacccgatc cccgccatgt
     7981 gcgagatggt gctggcgttg cagacgatga cgacacgtgg cttcgacgtg ttcgacccga
     8041 tcgtcgtgac ggtcggccgg atcgccggcg gcacgaagga gaacatcatc ccggacgaag
     8101 ccgagttcga ggccaccgtc cgatcgttgt cgccccagtc gcgggccaag gtccgagcag
     8161 acatccagcg cctggtcggc ggtatcgcgg cggcgcacgg actggcggtt gcggtcgact
     8221 acgagctcgg ctatccggtg accgtcaacg acgacacgga gtacgcccat gctctgcagg
     8281 tggtcgacga cctgttcggc agggacaggt tcaccgagct tccgacgcct gagctcggtt
     8341 cggaggacat ggccttcgtg atggagctcg tcccgagtgc ctacttcaac gtcagcgcct
     8401 gtccggtggc tgactacgcc aacgcgcccg acaaccactc gccgcgagcg gccttcgacg
     8461 acgcggtgct cccggatgct gcggcctggc tggcggaggt cgccgtacgc cgccttcgtc
     8521 gtgcgacacc cggccccgac tcggcctgac cgagatccgc gggcaggttc ggacgcaaca
     8581 ccgacggtcc acccgtgctg cccgcggtct agccttgccc gatggggttc gagtcctgga
     8641 tcgaccggca gatccgcgag gccgaggagc gtggcgagtt cgacaacctg cccggagccg
     8701 gcaagccgct gaaggatctc gacaagccgc aggacgcgga ctggtgggtg aagcggctca
     8761 tcgcccggga aaacctcccc ccggtcctac cgacggcgct ggcgcttcgc aaggaggtcg
     8821 aggacctgca ggagacgctt cgaccgctct ggcgggagag cgatgtgcga cacgttgtcg
     8881 acgacctcaa ccagcgcatc atcgatgcgc ggcgacggcc gtcggccggc cctgcggtgt
     8941 tcgtgaagac tgttgccgtg gacgaggccg tcgagcgctg gcgggcggac cgcgagcgac
     9001 gactcggcgg gggtgcccgc cggtccgagc ccgccgacgg cgggcagcgg catgaccgcg
     9061 gacgtcgacg ctggttcgga cgtcgccgca gctgacggat gtgccagaca cgcgtcgtca
     9121 gccgttgacc gtcgaccggg tcgacaagga tgagcaccgt gaaccccgac gacggtcgcc
     9181 cgcgagctgc gcacgacgag cgactcgagc cagacacgtg ggggatccac cggtgctgga
     9241 tgaacagcgc ggacgagctc gtcgaggtgc ctgggcggac gatcgagcgg ctccggacga
     9301 tcatcggtcg gccgcctgac gacctcgcgg agcgggcgcc actggtgact cagcctggtc
     9361 gtgacctccg gctcggtgtc ctcgacgtcg agtgtgagga cggtcagcgt cgcacgatcg
     9421 acggaatcgt cccggatgac ttccccctgg gctaccacga caccactgtg ggaggaatcc
     9481 gacgtcgact gatcgtgtca ccgggcagct gctggttgcc gccgggccgg catggctggg
     9541 gatgggcggt gcagctgtac gccgcccggt ctcgcgacag cggcggcatc ggcgacctgg
     9601 cggacctgcg cacgatccgc gtgtgggcgc aagggctggg cgcgtcgttc ctcctggtga
     9661 acccgctgca cgcggtcgcg ccgacgttcc cccaggagcc gagcccctac ctcccggcga
     9721 gccggcgctt ccgcaacccg ctctacctgc gcgtggctca cgtcgcacac accaatccgg
     9781 tcgacgagct gatcgaccgt gacgccgtgt ggcgtgcgaa gcgcacggca ctcgaggcgg
     9841 agttcgcgac ccgtagcccc ggtgcggcgt tccatcggtg gcgagcggag caagggatgg
     9901 cgctggaggc gttcgcgacc tggtgcgtgc tggccgaaca ccatggtccg gactggcagg
     9961 cgtggccgac gggcctgcac gacccgaacg gagccggcgt acgcgggttc gcgcgcgagc
    10021 atgccgaacg tgtgacgttc cacgcctggc tgcagtggcg gttggacgag cagttcaccg
    10081 cggctgcggg tgagacccgc atcgtccaag acctgccggt cggtgtcgac gggggcggcg
    10141 ccgacgcctg gatgtggcag gagacggtgg cggcgggggc gacggtggga gcacccccgg
    10201 acgtcttcaa cgcgcagggg cagggctggg gctcaccgcc gatggcgccg tggcggctgc
    10261 gcctggcgaa ctatgagccg ttcgtggagt cgatccgggc caccatgtcg ggtggtggcg
    10321 gcatccggat cgaccacgtg atggggctgt tccggctctg gtggatcccg gagggcttgc
    10381 caccgagcga cggtgcctac gtgcgatacc cgtcccagga cctgctcgac atcgtcgcgc
    10441 tggagagcca ccgcgctcag gcggtcgtcg tgggcgagga cctggggacg gtcgagcccg
    10501 gcgtacgcga gacgttgcag gagcgagccg tgctgtcgta ccggctcctg tggttcgagg
    10561 acgctccgcc cgaggactgg ccggagctgg ccatggcggc catcaccacg cacgacctgc
    10621 cgacggtggc cgggctgtgg accggagctg acctcgagga ccaggtcgcc aacctctcga
    10681 cgccgcgacc cgcgctcgaa cgcgaccgtg ccgcgctgct gtcccgtctc ccgtcgatgc
    10741 ctgaaggagc cgacggcgtc gccctcgccg ctcatcgaac gctggccggc gcatcaagca
    10801 tgatgatctc ggcgactctc gaggacgccg tcgggcagca gttgcgaccg aacatgccag
    10861 gcacgcgtac gcgcccgaac tggtccatcc cgctgcccgt ccgcgtcgac gacctgccga
    10921 accatgccca gaccagcgcc atcgcgaaga tcctgcagcg tgggttgccg gcaccctccg
    10981 gacgtgacta gaacatatgt tcgaatcggc gtaccgtgga tgcatgggca cacacttcca
    11041 gggctcgctg ctcgacagtc ccgagctcgc ggcgccgagc ctcggtgacc tgggcgggct
    11101 gcgcaggacc cgactggcac gtggggcgtg ggtggacgtg ctcccgacgt gggtgcacgg
    11161 ttcggatggc ttgttcgagc ggctcatcga gaccgtcccg tggtacgccg agaggcgcca
    11221 gatgtacgaa cgcgtcgtcg acgtaccgcg cctgctctgc ttctacggag aggacgccga
    11281 cctcccggat ccgctgctcg acgaggcgcg ccgcgtgctc agcgcccact acgccgacga
    11341 gctcggtgag ccgttcacga cggcgggcct ctgctactac cgggacggtc gcgactcggt
    11401 cgcgtggcac ggcgacacga tcggcaggtc caggactcag gacacgatgg tggcgatcgt
    11461 gtccttcggt tctccccgcc gcctgtcgct ccggccgagc ggaggtggga gctccatcgg
    11521 cttctcgctc ggtcatggcg acctgatcgt gatgggcggc tcctgccaac gcacgtggga
    11581 gcacgccatc ctcaagaccg cccgccccgc cggtccgcgc atctccgtcc agttccgccc
    11641 tcacaacgtc cgctgaccct cccgccgacg tcccaaggtc gaggtggcag ctttcgttgg
    11701 tgtttgacgg cgtgtcggac cgtggttgcc acctcgatgg gatgcgtcag agctctagtt
    11761 tgccgtgctt gggaatcgtg ggcgcctcga cctgcacggt gcccgggtag atcacgccgg
    11821 cgccggtgtt gagcagcacg acctcgtcgt caggggacac ccatcccgag ctcgccagag
    11881 cccgcaccgc cgacactgtg gcggcacctt cggggcacat gaacaagccc tccagacggc
    11941 ccacccgacc cagatctgtc agcaggtcct ggtcgtccac ggccaccgcg gtgccgcctg
    12001 tctcccggat cgcatcgagc accaggaagt cgcccaacgc tttcggtacg ttgatgccga
    12061 acgccacggt cgaggagtcc tcagccatga ccgactcgtc ggctcccgcg acgaaggcgt
    12121 cgacgatggg tgagcagccg gtcgactgca cgcagaccag tcgaggcagc ggaccgtcaa
    12181 cccagccgag ctcgcggatc tccgtcaacg ccttgaagat gccgatgagc ccgacgcctc
    12241 ccccggtcgg gtagacgatc acgtcaggca tccgccagcc gaactgctcg acgatctcca
    12301 accccatcgt cttcttgccc tcgatgcgat acggctcctt cagcgtggcc acctcgaacc
    12361 actcttcggt gctggtcgcg atcccatccg ccaccagccg tccggcgtcc gagatgagtc
    12421 cgtccaccag ccgcaggtcg ccaccggtgg ccacggtctc ggcgcgggtg atggtcgggg
    12481 cgtccaccgg catcgcgatc gtcgcgatga gaccggcccg agcggcgtac gccgaccagg
    12541 cggccccggc gttcccgttc gtcggcatcg ccagtcgtcg cgctccgagc tccttcgccc
    12601 gggagactcc gacggcggca ccacgggcct tgaaggtccc ggtcgggagt agcccgtcgt
    12661 ccttgatcag cacacggtgc aggccgagat cggcggcgag cctcggcgcc tccaacagcg
    12721 cggtcatgcc ctcccccaag gtgacgacat gctcgggcgc agacaccggc agcagctcgt
    12781 ggtaccgcca caggtcgggc ggtcgcccgg agacgtcgtc tcgtcgtacg tcgactgcgc
    12841 tgaggtcata cctggccagc agcggcgacc cgtccgcccg gcaaaggccc tgcaggaccg
    12901 acgcgtcgta ctgcgtactg catcgagggc actcgagatg cgccaacgca ctgaagacca
    12961 tgtcgttgat ccaagcaaac cgacgagttc cccggacggt caccagtccg ccggagcaac
    13021 cagaactcac ggttcaacga accagaactc acggttcgac gaaccacaag tcacggttca
    13081 acgaaggagg tgggggatag gggttcttga gcgcgctaag gtcgcggaca tgtcggccgt
    13141 cgatgtatgg gactggcggc gccgcacgtt cgtgttgtac gccgaggtcc gcgcccacac
    13201 cgacaccgcc gccgcacacc gccactggat cgagcagcgc gacgtgatgc tccgatccca
    13261 tccggcgtca cccgtcccag cccgcgcgca gtcgtcatat ccgggggccg aggtcgcggc
    13321 gtacgacgcc gcacaccggt tcgtcgtgcg catcgaccgc gacgtcgagc cggagaccat
    13381 cgagtaccag accggcaccg acggcgtggt ccccttcgat cggatcggac gggtgcatct
    13441 gccggggctg ggaagcctcg acgtttggtg gctggcatcg tacggcggtg ggctgttcct
    13501 gccgttccgg gaccgcgcga gcacgtcgta cggcggcgga cgctacctgc tcgacacggt
    13561 caaaggggct gacctcggtg gtgacgcgga cgcactggtg gtggacctga acttcgcgta
    13621 ccagccgtcc tgcgcgtacg acgaggcatg ggcctgcccg ttggcgggcc cgacgaacac
    13681 gctcgatgtc gaggtgccgg tcggagagcg acatgtccgt ctcagcgacg acacagacgg
    13741 gagcacgcga tgacgatccc gacgatcggg acgctcgagc cgcttccggc agcggaacgt
    13801 cccgacctgc tggccgaccc agttcggaag gccttggccg gctgggagcg ggctggcggc
    13861 gtgggggtct tcgagatcga tcccgagctt gcggacacgc aggcgctgac cgacgcgtac
    13921 gacctcccgc tcgcagcatc ggccaactgt gtggtcgtcg ccggccggcg gggcggtgac
    13981 gagcgcatcg ccgcttgtgt cgtcaccgcc gacagacgcg cggacgtgaa caatctcgtc
    14041 cgccggatgc tcgacgttcg caaggcgtcg ttcctgtcga ccgaccgggc ggtcgaggag
    14101 acgtcgatgg agtacggcgg gatcactccg atcggcgtac cggcgcgctg gcggctgctc
    14161 gtcgacgctt ccgtggcccg cctcgacgtc gcgatcatcg gcagcggggt acgtcgttcg
    14221 aagctgctgc tgtccggagc cgacctcgcc gcataccccg gtgccgagct cgtcgacggt
    14281 ctgggcctac cggtgcagcc gcgttgagga cctgtcgcgt ttgttcaagc gcttcggcgc
    14341 gaagccgggc agcatggggt tcatgagcga agtctccagc cgtttcgcca ggctcgccgc
    14401 gaccatgacc gaccgcatct cggcggtgga cgacggggcg tgggagaacc cgtcaccgtg
    14461 ccagggctgg acggcgcgcg acctggtcca gcatctcgtc gacacgtacg ggatgttcgg
    14521 cggcttcgtc gaccagcagc tgacgcctgg atccgaggtg gcggaagacc cgctcggcgc
    14581 ctggatctcg gcccgcgacc agatgcaggc ctggctcgac gatccggcgg tcgcgaagcg
    14641 ggaggtccag ggagtcagcg gcacgacgac gttcgaggcg tcggtcaacc gcttcatctg
    14701 tttcgatctg ctcgtgcatg gctgggatct ggcgaaggcc accggccagg acaccaccat
    14761 cgacccggcc gagctctcca acctgtgggc tgacaccgag gcgttcggcg acaacatccg
    14821 taccggtggc gtctgcggcc ccgagctgcc cgccccggaa gacgcgacgg agcaggagcg
    14881 cttgctcgcc ttcctggggc gcgacgcgcg ggcctgagga tttctcccgg caggttgcgc
    14941 aaccagtcgg gcgtggaccg acccgcaggg ctcgccaccc taggatcagg acgtgtcagc
    15001 cctgctcgag gtcacgactg cagacgtcgt acgccgtcgg agcctacgtc ggatgcgcac
    15061 ggtcgcgacc tgcctgctgc tgttcgccgc cgtcgtctac gtcgccaccc tgcatcggtc
    15121 gggcggctgg gcgtatctcc acgcaacggc cgaggcggcg atggtgggtg cgatcgcgga
    15181 ctggttcgcc gtcacggcgt tgtttcgtca cccgctcggg ataccgattc cgcacaccgc
    15241 gctgatcccg acccgcaaga acatgctggc gcgcagcctg caggacttcg tgacgcagaa
    15301 ctttctccag gagtcggtgg tccgcacccg cgtcagtgat gcccaggtga gtcaccgtgt
    15361 gggggagtgg ctccgacagc ccggtcacag cgcccgggtc gtgtcggagg ccgccgcgct
    15421 gatcgagatc ggcctcgcca ggatccggga cgaggacgtg gactacttcg tccgtggcga
    15481 gctgcttccc cggttgtccg aggaacggct ggcgccgctg gccggccagc tgctcggcga
    15541 cgtcgtggcg gagggatcgc accatggtct cgtcgacctc gtgctggccg aggcggaccg
    15601 gtggctggca acgaacgagg cggcttttgc gggcgccgtc ttgacgaggg ctccctggtg
    15661 gtcgccgacc tggctggacg agaaggtggt cgcccgtctg caccgggagg tgctcgcgtg
    15721 ggtccgtgag atccgcgacg acccctccca tcatgcccgc cgtgcgttgg acgacctgct
    15781 ctcccggctc gccaaggatc tgtgtgagga cgtggacacc caagcgcgag cggagcgcct
    15841 caagcagcgg atcctcgcgc agccgcaggt cgccgacacc gcgctctcgc tgtggaaggc
    15901 actcagcagg accttgcaga ccagcgttcg cgaccctgag ggagccctgc gacagcgcgc
    15961 ggtgcaccgg ctccagacct tcggcgaaca gctggtgagc gataccgcgc tccaggcgcg
    16021 gctcgacgaa catgccgccg acctggcggc gttcgtcgtc gggcggtacg gcgacgagct
    16081 ggcgaccgtc atcacagaca cggtcgagcg ctgggacgga cgcgaagcgg ccagtcgtat
    16141 cgagctgtac gtcggccggg accttcagtt catccgcatc aacggaaccc tcgtcggcgg
    16201 cctcgcgggt ctggtcatct acagcgtcgc cgagctcctg tgacctccgg atcggctgag
    16261 tcgacccgat cgggcgttgc gtgctgagcc gcagcgagct tgtcgaaggc cacgggctca
    16321 gggagcggaa ggcgctgtac cgctgaggct cttgccgagg tggacggccg ctggctcgtc
    16381 cttgtagtgg ccgaagcgcg ggatctccga gtatccgctc ccgaggtaga gcccgacggc
    16441 gtccacctgc gggctgcctg tctccagcac gagcatgtcg gcgcccgcgg ccaaggcaga
    16501 ctgctcgaga tggcggagca tcgtcttgcc gagtcctgtg ccgcgatgtg ccggcacgac
    16561 atacatccgt ttgagctcgg cgggccgctg cgcgggcacg tccaacgcct cgatcgcgaa
    16621 ccgccagcca cccatcacga ccggctcgcc ggcgaggtag ccgatgaaga acgcgccgtc
    16681 cggccgggcg agctcacgcg ggtcgatggg cgaggtgtcg ggtccgccgt agatcaggac
    16741 gtagtactcc tgcacgtcgg agatgagggc cagagcatcg ggatggtcga gggtgcgttc
    16801 acggacctcg agcgcatcca accgcgactt cacgccgcca cgctaaccac tcgcgagcat
    16861 caccgatcgc gcgtggtcca gtcagcaccg ccgatgatct cggctcccga gtggttggag
    16921 ggccggagtg cgcggcatcg tcgggggtgg gtaggccggc gatgccgcgc agtggggtgg
    16981 ccgtacgcgc tcgccctgat gcgtgacggc actctgcctc cacagtcctc tcgccgctcg
    17041 cgtcgcgcaa gggaaattcc acgcacgccg gcaggcgagc agcgcggcgg caatcagacg
    17101 cgtgggtcag cgccgcagcg cgttgctcac cggaatggcg agtgccgaga cgccgacgat
    17161 cacgaggctc acaccggtga gtgcgccgcc ctgaccgagc gagccggctg cccacgtgag
    17221 gtacaaggtg ccgatcgt
//
LOCUS       DATBFZ010000109        11024 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_2361165, whole genome shotgun sequence.
ACCESSION   DATBFZ010000109 DATBFZ010000000
VERSION     DATBFZ010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 11024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11024
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2361165"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            96..167
                     /locus_tag="VH419_03315"
     tRNA            96..167
                     /locus_tag="VH419_03315"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:129..131,aa:Arg,seq:ccg)
     gene            473..2695
                     /locus_tag="VH419_03320"
     CDS             473..2695
                     /locus_tag="VH419_03320"
                     /inference="COORDINATES: protein motif:HMM:NF039646.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6077 domain-containing protein"
                     /protein_id="HEY7042677.1"
                     /translation="MTGREDSDSAADSTAGTEAAPVPTELTRTSPAAPTMTRDQTYAA
                     ASADAMPAPAPLRQLWPDRLTRAVDLTLDGVVVLLATWTVVYHVCLILGLRVPWAVGL
                     EITLLVLVAVARHRTRRWREVRVSAPVPAYADTGTRTVDGVLTEPRLVGVTVAAIAVS
                     AAAMATDAPWTFVWVSWLVGASSGTVWAWRRLRDNGWRADPDSEQDDVALPHVWHPRS
                     GRTAGVVSALLWAVGLAVLSTCMLRPNPDDLYYVNLSQWVVEHGDFPTRDTIFSDQVF
                     PMSSWPPVASYDALVGVVARLTGTAAASVAYVAVLPLATVLSVLALWRLLRAWRIGPV
                     ALALSAALIFLLFDGAEEYSTPGGLLLAGYATPGGLFLTRLWQGKVILLCVLVPLLLV
                     YGLRYVERPTWRRLVWVFVAGTAAVGLSTSAIFLVPLVAVACAAPLVRVAPRAALAGF
                     AAMAAYPLGAGVVTKALGGRSADNFGERKLYRFAPHWFGHEIFQSGIVAVIGVTAVLL
                     GALLVTHRQARLTTGLLALVTGLTFLPGVTHLAYDVIGLGPTLWRVSWVASIAALVGV
                     LATHVARLLRARWARWGAGVASVGLLLAFGVPTWSGVTNTSWEAPFHWQRDASTMALA
                     DKIIDGSREGALVLAPDDLAITIDITTTKVKTAAPRDYVMDYLRDDPAFHYDERLVLV
                     DLVNEVGPWHAAAVIHALRVLDVDVACVYADDGRRYRTIVDAGYTPWVESSAYRCVRR
                     "
     gene            2715..4226
                     /locus_tag="VH419_03325"
     CDS             2715..4226
                     /locus_tag="VH419_03325"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HEY7042678.1"
                     /translation="MTMDRDVATPSTTAPRDTPDQGVGRLGHEMWVAHRPFLIALGLG
                     AVMRLVVSIAFPSAFIQGDGPTYLGLVDHFAVDPDRPVGYGVPLRVLSWISRDVTLVA
                     ITQHLLGLATAVLVYALLRRWGVSGPIATLATLPVLFDSMQLVLEHTILSDVVFDFAI
                     VLGISLLAWHRTPRARDAFVAGLVLGAAVLVRVVGEPVLIAGIVFCLMACHAWRPRLV
                     TSAALCIAFLLPIAAYATWYHESRGVWALTEFGGKALYMRTTTFVDCSRFSMPEYEAA
                     LCPIDPLGERQDPTEYGWHDPTTIPALEPPPGVSDDEAMHAFATRAIRAQPLDYLRVA
                     TRDFAMNFYPERKDLAEYDTADKWLFETWVDYEPTDWTGPAYDAHGGPMHESLQPIAG
                     LVAAYGQKVFLRGPLLLGCLLLALCGIAVRRRSVVPDVRPLAVLTVAVGTGLMLVPDL
                     TAEFIWRYQLPAVILLPMAAALGWTRLRTPQPDPPSARDEPLVSTSSARGQGR"
     gene            4292..4666
                     /locus_tag="VH419_03330"
     CDS             4292..4666
                     /locus_tag="VH419_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020389455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3052 domain-containing protein"
                     /protein_id="HEY7042679.1"
                     /translation="MKPGHRVYLDNVPDTLMLEWPDDVVVVRRLPPAQRTSLDVAWTF
                     CADRARLERRLEPIAQRLASNGMVWVSWPKKGSGVATDLDENVVRAIGLGAGLVDVKV
                     AAVDETWSGLKFVYRLADRPTA"
     gene            complement(4729..5610)
                     /locus_tag="VH419_03335"
     CDS             complement(4729..5610)
                     /locus_tag="VH419_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEY7042680.1"
                     /translation="MRRLALFGLLLAVLGFVIPDSGRAPAQFALIKLDEVHGVDSTDD
                     VVWILALGSDARPGQPILGSRSDAIQLVGINTRNHHAVTIGVPRDSYVDIPGAGRDKI
                     NSAMVYGGPQATAQAVGNLVGIQPDYVFVTSFPGLIKMVSGIHGIDVKVTFEMNDLGH
                     VFHPGKTHLAGRDALAFSRIRHGLPRGDFDRSHDQGQVLKGGLATIKDKLGRPGFLER
                     ALLLFAQNTDTNLTPVQLYRLAHTVLEVNPKFVRTCVVTGGTGFAGSASVVFPDVAAA
                     RALGEDVRHDATVNGSC"
     gene            complement(5688..6290)
                     /locus_tag="VH419_03340"
     CDS             complement(5688..6290)
                     /locus_tag="VH419_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042681.1"
                     /translation="MRITKSGLVAFLAMVVAVTIVATSCTHHESSDSPPATSTAGATT
                     LDKNNAELRVSFVELEGVRKRMRAGLVRGIGEPIRAWMDAAYLAGDFPLSSYTGKAFP
                     GWTPGAARLGLRDRRVTTNAAISKDVVNVVADQRSARLAVFGVRGRTGGADAQIHLKM
                     TAKKSSGQLVSFAVAGHLYLTRKADHWSIFGYDLHRTVLR"
     gene            6457..10347
                     /locus_tag="VH419_03345"
     CDS             6457..10347
                     /locus_tag="VH419_03345"
                     /EC_number="4.1.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007077281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional oxoglutarate
                     decarboxylase/oxoglutarate dehydrogenase thiamine
                     pyrophosphate-binding subunit/dihydrolipoyllysine-residue
                     succinyltransferase subunit"
                     /protein_id="HEY7042682.1"
                     /translation="MASATQRKRRRSTVSQTPHDSSTSTRPAEVDTDFGPNEWLVDEM
                     YDRYVQDPDSVDEAWRELFKDRRPNGQPADVNATTSQTAAPAPSTAPPAGATGAASKA
                     AESTTRRAEPGPAPTPKATTPSPSPAATTGSAAASSATPAAATDKRTPAAPPAATQPK
                     PAPVAKDQPAASTDSATVTEPQHVVLRGASARTVTNMDTSLNVPTATSVRTVPVKLLI
                     DNRVVINNHLARARGGKVSFTHVIGFALVKALKTMPEMNYGFEVVDGKPNLVKPNHVN
                     LGLAIDVQKADGTRQLLVPSIKAAEDMGFAQFWSAYEDIVRRARDNKLTLEDFQGTTI
                     SLTNPGTIGTNHSVPRLMSGQGCIVGVGAMNFPPEYQGASEETLNRNAISKIMTLTST
                     YDHRIIQGAQSGDFLRRLHALLLGEDAFYDEIFRALRIPYEPIRWASDISASHEDQVS
                     KQARILEMIHAYRVRGHIMADTDPLEYKQRSHPDLDVTTHGLTLWDLDREFATGNFGG
                     QRFMKLRHILGILRDSYCRTTGIEYMHIQDPEQRRWIQERVERPHKKPPREEQLRILH
                     KLNQAEAFEVFLQTKFVGQKRFSLEGGETTIPLIDEICEAAADHSLDEVCIGMAHRGR
                     LNVLANIVGKRYSQIFREFEGNIDPRTVQGSGDVKYHLGAEGEFVSGNGSTIKVSVAA
                     NPSHLEAVDPVLEGIARAKQDLLNQGAAYPVLPLLVHGDAAFAGQGVVAETLNLSQLR
                     GYRTGGTIHVVVNNQVGFTTSPSSSRSSLYCTDVARMVQAPIFHVNGDDPEACIRVAQ
                     LAFEYRQAFNKDVVIDLVCYRRRGHNEGDDPSYTQPLMYDLIEQKRSVRKLYTEALIG
                     RGDITVEEAESALQDYQQQLERVFAEVRDAKNEPEKLPEFVTVPEYPTKAVPGSTDTA
                     VSREVLKRIAEAYVTPPEGFTVHPKVLPQLQRRSASITDGPIDWGTGEILAFGSLLLQ
                     GRPVRLSGQDSRRGTFVSRFATIIDRKNGAEWTPLQNLSADQAQFYIYDSLLSEYAAM
                     GFEYGYSVARPEALTLWEAQFGDFTNGAQTVIDEFVSSGEAKWQQQSGVVLLLPHGYE
                     GQGPDHSSARIERFLTLAAGDAFTMAQPSTPASYFHLLRRHSLGSKHKPMIVFTPKSM
                     MRRKDAASQPDDFTSGTFAPVIGDDVDDPNAVERLLLCSGRICWDLLAERKRREDGQS
                     RTAIARLEQLYPRPVDEVQAELAKYPNLREIRWVQDEPANMGPWPHVKLNLAPELGDV
                     PFQRISRPESAAPSVGQSAVHQQELQNILDETFA"
     gene            10373..10555
                     /locus_tag="VH419_03350"
     CDS             10373..10555
                     /locus_tag="VH419_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002518904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6104 family protein"
                     /protein_id="HEY7042683.1"
                     /translation="MYFTDRGIEELTDRRGDEEVTLAWLAERLSEFVDLNPEFETPVE
                     RLATWLARLDNEDDLD"
BASE COUNT         1807 a         3771 c         3721 g         1725 t
ORIGIN      
        1 tgctcacggc gctgggcggc acgccgctgc cgccgccgtc ctgacggccg ccgaccgacc
       61 cggccgggac ggctggtaac ttctctcctg cccgagcccc cgtagctcag gggatagagc
      121 accgccctcc ggaggcgggt gcgcaggttc gaatcctgcc gggggcgcaa gccacctgac
      181 accctgacca ccttcgttcg ggaaccacct ccgggagggc cagcctccgg cagcgaatca
      241 cctacggaag ggccggcctc agccaccgtg ccccgcgctt cacgacgatc gggctctcca
      301 aacgtccgga agggcaagtc cccaccggag gacccaactc cgaggtggca atggcgttcc
      361 cagacacggc gtgtttgcaa cgaaacctgc cacctcggcg ggagatgagg aacgcgtggc
      421 gcggtcgagg cggtgcgcgc gtgaccaggt cagcccgggt tacattggcg gcgtgacggg
      481 gcgagaagac agtgactcgg ctgccgactc gacagccggc accgaggccg cgcccgtccc
      541 caccgagctg acgcggacat cacccgccgc tcccaccatg acccgcgacc agacgtacgc
      601 cgctgcgtca gcggacgcga tgcccgctcc ggcgccactg cgtcagctct ggccggaccg
      661 gctgacacgc gcggtcgacc tgacgctgga cggcgtggtc gtcctgctcg caacgtggac
      721 cgtcgtctac cacgtctgcc tgatcctggg actgcgggtg ccgtgggccg tagggctcga
      781 gatcacgctt ctggtactcg tggcggtcgc ccgtcaccgg acccgtcgat ggcgcgaggt
      841 ccgggtgtct gcgccggtcc cggcgtacgc cgacaccggg acgagaacgg tcgacggggt
      901 gctcacggag ccgcgtcttg tcggggtcac cgtggctgct atcgcggtgt cagccgcggc
      961 catggcgacc gacgccccgt ggacgttcgt gtgggtcagc tggctcgtgg gggcgagctc
     1021 cggaaccgtc tgggcgtggc gccgactgcg cgacaacggc tggcgggccg accccgactc
     1081 cgagcaggac gacgtcgcgc tgccccacgt ctggcacccc cgcagcggcc gcaccgcggg
     1141 cgtcgtgtca gccctgctgt gggcggtcgg gctcgcggtg ctgtcgacgt gcatgctgcg
     1201 gccgaacccc gacgacctct actacgtcaa cctgtcccag tgggtcgtcg agcacgggga
     1261 cttccctacc cgcgacacga tcttctccga ccaggtgttc ccgatgtcgt cgtggccgcc
     1321 cgtcgcgtcg tacgacgccc tggtgggcgt ggtcgcgcgg ctcacgggta cggcggcggc
     1381 atcggtggcg tacgtcgccg tgctgccact cgcgacggtg ttgtcggtcc tggcactgtg
     1441 gcggttgctg cgcgcctggc ggatcggtcc ggtcgccctc gcactgtccg cggcactgat
     1501 cttcctgctg ttcgacggcg ccgaggagta cagcacgccg ggcggactgc tcctcgccgg
     1561 gtacgcgacg ccgggcggcc tcttcctcac acggctgtgg cagggcaagg tgatcctgct
     1621 ctgcgtactc gtcccgctgc tcctggtcta cggactccga tacgtcgaga gacccacttg
     1681 gcggcgactg gtctgggtgt tcgtggccgg gaccgccgcc gtcggactgt cgacgtcggc
     1741 gatcttcctg gttccgctgg tcgcggttgc ctgcgccgca ccgctggtcc gggtcgcccc
     1801 acgggcggcg ctcgcgggat tcgcagcgat ggccgcctac ccgcttggtg caggagtggt
     1861 cacgaaggcg ctgggcggac ggtccgccga caacttcggg gagcgcaagc tctaccggtt
     1921 cgccccgcac tggttcgggc acgagatctt ccagagtggg atcgtcgccg tcataggagt
     1981 caccgccgtc ctgctcggtg cactgctggt cacgcatcga caagcaaggc tgacgacggg
     2041 cctgctggcc ctggtgacgg gactgacgtt cctccccggc gtcacgcacc tggcgtacga
     2101 cgtgatcggt ctcggtccga cgctgtggcg ggtgtcgtgg gtcgcctcga tcgccgcgct
     2161 ggtcggcgtg ctcgcgacgc atgtcgcacg tctgctgcgg gcgcgctggg cacgatgggg
     2221 cgctggcgtc gcgtccgtcg ggttgctgct cgccttcggt gtgcccacgt ggtccggtgt
     2281 caccaacacc tcgtgggagg ctccgttcca ctggcagcgc gacgcctcga ccatggccct
     2341 cgccgacaag atcatcgacg gcagccgcga gggggccctg gtcctcgcac cagacgacct
     2401 cgcgatcacc atcgacatca ccacgaccaa ggtcaagact gccgcccctc gcgactacgt
     2461 gatggactac ctgcgtgacg atccagcctt ccactacgac gagcgactgg tactggtcga
     2521 tctcgtgaac gaggtgggcc cgtggcacgc cgcggcagtg atccatgcgc tgcgcgttct
     2581 cgacgtcgac gtcgcgtgcg tgtacgccga cgacggccgg cggtaccgaa cgatcgtcga
     2641 cgccggatac accccgtggg ttgaatcctc ggcctaccgg tgcgtgcggc gatgaggcag
     2701 ccgagggatc cttcatgacc atggaccgcg acgtggccac gccgagcacg acggcgccgc
     2761 gcgacacgcc cgaccagggc gtcggtcggc ttggtcacga gatgtgggtc gcgcaccggc
     2821 cgttcctgat cgcgctcgga ctgggcgcgg tgatgcgtct ggtcgtctcg atcgctttcc
     2881 cgtcggcctt catccaggga gacggcccga catacctcgg actggtcgac cacttcgcgg
     2941 tcgatcccga ccggcctgtc ggctacggcg tgccgctgcg cgtgctgtcc tggatcagtc
     3001 gtgacgtcac gctggtggcg atcacgcagc acctcctcgg gctcgcgaca gccgtactcg
     3061 tgtacgcgct gctgcggcgc tggggcgtgt ccggacccat tgccaccttg gcgacgctcc
     3121 cggtgctgtt cgacagcatg cagctcgtgc tcgagcacac catcctcagc gacgtcgtct
     3181 tcgacttcgc catcgtgctc ggcatctccc tcctcgcctg gcatcgcacg ccccgggcgc
     3241 gggatgcgtt cgtggctggc ctcgtgctgg gagccgcagt gctggtgcgc gtggtgggcg
     3301 agccggtgtt gatcgcaggc atcgtcttct gcctgatggc gtgccacgcg tggcgtccgc
     3361 gcctggtcac cagcgccgcg ttgtgcatcg ccttcctgct gccgatcgcg gcgtacgcga
     3421 cctggtatca cgaaagccgg ggcgtctggg cgctgaccga gttcggcggc aaggccctct
     3481 acatgcgcac gacgaccttc gtcgactgtt cgcggttctc catgccggag tacgaagccg
     3541 cgctgtgccc gatcgacccg ctcggggagc ggcaggaccc caccgagtac ggatggcacg
     3601 acccgacgac gattcccgca ctcgagccgc ctcccggggt gagcgacgac gaggcgatgc
     3661 acgccttcgc gacgcgggcc attcgggctc agccgctgga ctacctgcgt gtcgccaccc
     3721 gggacttcgc gatgaacttc tacccagagc gaaaggacct cgcggagtac gacaccgccg
     3781 acaagtggct gttcgagacg tgggtcgact acgagccgac ggactggaca ggtcctgcct
     3841 atgacgcgca tggcggcccg atgcacgaga gcctgcagcc gatcgccggg ctcgtcgcgg
     3901 cgtacgggca gaaggtgttt ctgcgcggtc ctctcctatt ggggtgcctg ctgctcgcgc
     3961 tgtgcgggat cgcggtacgc cgccggtccg tggtcccgga cgtgcgcccg ctggccgtcc
     4021 tgaccgtcgc ggtcggtacc ggactcatgc tggtgcccga cctgaccgcg gagttcatct
     4081 ggcgctacca gctcccggcc gtgatactgc tcccgatggc ggcggcactc ggctggaccc
     4141 ggttgcgcac gccgcagccc gacccgccca gcgcacgtga cgagcccctg gtgtcgacga
     4201 gctcggcacg cggtcagggc cgttgacccc gaccggcgcg aacagcggac cgacgggctg
     4261 ttccgggaaa ccgctgctct cgaagatcgg cgtgaagccc gggcaccggg tgtacctgga
     4321 caacgtcccg gacacactca tgctcgagtg gccggacgac gtggtcgtcg tacgccgtct
     4381 gccgcccgcg cagaggacct cgctcgacgt ggcctggacg ttctgcgctg atcgggcccg
     4441 actcgaacgt cggctcgagc cgatcgccca acggttggcg agcaacggca tggtctgggt
     4501 gtcgtggccc aagaagggct cgggcgtggc gaccgacctc gacgagaacg tggttcgcgc
     4561 gatcgggctc ggcgcaggtc ttgtcgacgt caaggtcgcc gccgtggacg agacgtggtc
     4621 aggcctgaag ttcgtgtacc ggttggctga ccgaccgacg gcatgacccg aactgggcac
     4681 tgccgtcgcg acccggctac gagccgccga cggtgcaacc ggcccggact agcaggagcc
     4741 gttgacggtt gcgtcgtgcc gcacgtcttc tcccagcgcc cgtgccgcgg cgacatcggg
     4801 gaagacgacg ctcgccgagc cggcgaaccc ggtgccaccg gtgaccacgc acgtgcggac
     4861 gaacttcggg ttcacctcga gcacggtgtg ggcgagtcgg tagagctgga ccggtgtcag
     4921 gttcgtgtcg gtgttctggg cgaagagcag cagcgcgcgt tcgaggaacc ccggtcggcc
     4981 gagcttgtcc ttgatcgtcg cgaggccgcc cttcaggacc tggccctggt cgtgggaacg
     5041 gtcgaagtcg ccgcgcggca acccgtgccg gatccgcgag aacgccaacg cgtcgcggcc
     5101 ggccaggtgc gtcttgccgg ggtggaacac gtgccccagg tcgttcatct cgaacgtcac
     5161 cttgacgtcg atgccgtgga tgcccgagac catcttgatc aagccaggaa agctcgtcac
     5221 gaacacgtag tcgggctgga tgccgacgag gtttccgacg gcctgcgcgg ttgcctgcgg
     5281 gccgccgtag accatggctg agttgatctt gtcgcgcccg gcacccggga tgtcgacgta
     5341 ggagtcacgg ggtacgccga tcgtgaccgc gtggtggttc ctggtgttga tcccgaccag
     5401 ctgaatggcg tcggagcgtg aaccgaggat cggctgccct ggccgcgcgt ccgagccgag
     5461 agcgaggatc cagacgacgt cgtcggtgga gtcgacccca tggacctcgt ccagcttgat
     5521 gagtgcgaac tgagcgggtg cacgccccga gtcggggatc acgaaaccga gaacggcgag
     5581 cagcaggccg aacagggcga ggcgtcgcac gcggcgaacc gtacgtcgcg cacgctcgcc
     5641 ggcagtggaa gcgtgccttt ccggcaccga agaggtcagc agaatcatca gcgcagcacc
     5701 gtccggtgga ggtcgtagcc gaagatgctc cagtggtcgg ccttgcgggt caggtacagg
     5761 tgaccggcga cggcgaagga caccaactgc ccgctgctct tcttggctgt catcttgaga
     5821 tggatttggg cgtcggctcc gcccgtgcgt ccacggaccc cgaacaccgc cagccgcgct
     5881 gagcgctggt ccgccacgac gttgaccacg tccttgctga tggccgcgtt ggtcgtgacg
     5941 cgccggtcgc gcaagccgag tcgggcggcg ccgggcgtcc agcccgggaa cgcctttcct
     6001 gtgtagctcg acagcgggaa gtcgccggcc aggtacgccg catccatcca ggcgcgaatc
     6061 ggctcaccga tacctcgtac gaggccggca cgcatccgct tcctgacccc ctcgagctcc
     6121 acgaacgaga cgcggagctc ggcgttgttc ttgtccaagg tcgtcgcccc cgcagtcgac
     6181 gtggcaggcg gcgagtccga cgactcgtgg tgggtgcacg acgtggccac gatcgtcacc
     6241 gcgacgacca tggccaagaa ggccaccagg cccgacttgg tgatgcgcat cgggagcatg
     6301 atgcccaaga ggcaggtcaa actcaactca gccgcgcgac attgagccgg atggccgatg
     6361 gccttggtgc ccgcccgggt cgcggcatcg tggacttggc tgcagcctgg gaggacgatt
     6421 gcccgagcga tagcctgggt cgagagcacg agcctgatgg catcagcgac gcagcggaag
     6481 aggcgaaggt ccaccgtgtc acaaaccccg cacgacagtt caacgtccac ccgaccagct
     6541 gaggtagaca cggacttcgg acccaacgag tggctggtcg acgagatgta cgaccgctat
     6601 gtccaggacc cggactccgt ggacgaggcg tggcgggagc tcttcaagga ccgccggccg
     6661 aacggtcagc ccgctgacgt caacgccacg acctcgcaga ccgctgcgcc ggcgccgtcg
     6721 actgcgccac cggccggtgc gaccggcgcg gcgtcgaagg ccgctgagag cacgacccgg
     6781 cgggcggagc ccgggcctgc tccgacaccg aaggcgacca cgccgagccc gtctcccgcc
     6841 gccacaacgg gttccgctgc cgcgtcgtcg gcaacacccg ccgctgcgac cgacaagcgg
     6901 actcctgcgg caccacctgc cgccacgcaa ccgaagcctg caccggtggc gaaggaccag
     6961 ccggcggcat cgaccgacag cgccaccgtg acggagccgc agcacgtcgt ccttcgcggc
     7021 gcgtcggcgc ggaccgtcac caacatggac accagcctca acgtcccgac ggcgacgagc
     7081 gtgcgaacgg tgccggtcaa gctcctgatc gacaaccgcg tggtgatcaa caaccacctc
     7141 gcccgcgcac gtggtggcaa ggtgtcgttc acgcacgtca tcggcttcgc gctggtcaaa
     7201 gcgctgaaga cgatgccgga gatgaactac ggcttcgagg tcgtcgacgg caagcccaac
     7261 ctcgtgaagc ccaaccacgt caacctcggc cttgcgatcg acgtgcagaa ggcggacggg
     7321 acccgacagc tgttggtgcc gtccatcaag gctgcagagg acatgggctt tgcgcagttc
     7381 tggtcggcgt acgaggacat cgtgcgtcgc gcccgcgaca acaagctcac gctcgaggac
     7441 ttccagggca ccacgatcag cctcaccaac ccggggacca tcggcaccaa ccattccgtg
     7501 ccgcgactga tgagtggcca gggctgcatc gtcggcgtcg gcgcgatgaa cttcccgccc
     7561 gagtaccagg gtgcgtccga ggagacgctg aaccgcaacg cgatcagcaa gatcatgacg
     7621 ctgacgtcga cgtacgacca ccgcatcatc caaggcgccc agtccgggga tttcctgcgt
     7681 cgcctgcatg ccctgctgct cggcgaggac gccttctacg acgagatctt ccgggcactg
     7741 cgtattccgt acgagcccat ccgttgggcc agcgacatct cggcaagcca tgaggaccag
     7801 gtcagcaagc aggccaggat cctcgagatg atccacgcct accgtgtgcg cggccacatc
     7861 atggccgaca ccgacccgct cgagtacaag cagcgcagcc atccagacct cgacgtcacg
     7921 acgcacggcc tcacgctctg ggatcttgac cgcgagttcg ccaccgggaa cttcggcggc
     7981 cagcggttca tgaagctgcg gcacatcctc ggcatcctgc gcgactcgta ctgccggacg
     8041 acgggcatcg agtacatgca catccaggac cccgagcagc gccggtggat ccaggagcgc
     8101 gtcgagcgtc cgcacaagaa gccaccgcgt gaggagcagc tgcgcatcct gcacaagctc
     8161 aaccaggcag aggcgttcga ggtgttcctg cagacgaagt tcgtcgggca gaagcggttc
     8221 agcctcgaag gtggagagac gaccatcccg ctgatcgacg agatctgcga agcggccgcc
     8281 gaccactcgc tcgacgaggt gtgcatcggt atggcgcacc gcggccggct gaacgtgctt
     8341 gccaacatcg tcggcaagcg gtactcacag atcttccgtg agttcgaggg caacatcgac
     8401 ccgcggaccg tccagggctc cggcgacgtg aagtaccacc ttggcgccga gggcgagttc
     8461 gtctcgggca acggcagcac gatcaaggtg tcggtcgccg ccaacccgtc gcatctcgag
     8521 gcggtcgacc cggtgctgga ggggatcgcg cgggccaagc aggacctgct caaccagggg
     8581 gcggcgtacc cggtgctccc cctgctcgtc cacggagacg cggcgttcgc cggacagggt
     8641 gtcgtggccg agacactgaa cctgtcgcag ctgcgcggct accgtaccgg cggcacgatc
     8701 cacgtggtcg tcaacaacca ggtggggttc accacgtcgc cgtcgtcgtc acgctcgtcg
     8761 ctgtactgca cggacgtcgc gcggatggtg caggcgccga tcttccatgt caacggtgac
     8821 gacccggagg cgtgcatccg ggtggcgcag ctcgcgttcg agtaccggca ggcgttcaac
     8881 aaggacgtcg tcatcgacct ggtgtgctac cgccggcgcg gtcacaacga aggggacgac
     8941 ccgtcgtaca cgcagccctt gatgtacgac ctgatcgagc agaagcgttc ggtccgcaag
     9001 ctctacaccg aggcgctgat cggccgtggt gacatcaccg tcgaggaagc cgagtcggcg
     9061 ctgcaggact accagcaaca actcgagcgc gtgttcgccg aggtccgcga cgcgaagaac
     9121 gaaccggaga agctaccgga gttcgtgacc gtgccggagt acccgaccaa ggcggtgccc
     9181 ggaagcaccg acacggctgt ctcgcgcgag gtgctcaagc ggatcgccga ggcgtacgtc
     9241 acgccgcccg aaggcttcac cgtgcacccg aaggtgctgc cgcagctgca acgtcggtcg
     9301 gcgtcgatca ccgacggccc gatcgactgg ggcaccggcg agatcctggc gttcggctcg
     9361 ctgctgctgc aggggcggcc ggtgcgactg tccggtcagg actcgcgaag aggcacgttc
     9421 gtcagccggt tcgccacgat catcgaccgc aagaacggcg ccgagtggac gcccctgcag
     9481 aacctgtcgg ctgaccaggc gcagttctac atctacgact cgctgctgtc cgagtacgcc
     9541 gcgatgggtt tcgagtacgg ctactccgtc gcgcggccgg aggcgctgac gttgtgggag
     9601 gcgcagttcg gcgacttcac gaacggcgcg cagacggtca tcgacgagtt cgtgtcgtcg
     9661 ggtgaggcga agtggcagca acagtccggc gtcgtactcc tgctccccca tggctacgaa
     9721 ggccagggtc ccgaccactc gtcggcacgc atcgagcgct tcctgacgct cgccgccggc
     9781 gacgcgttca ccatggcgca gccctcgacg ccggcgagct acttccactt gttgcgccgg
     9841 cactcgctcg gcagcaagca caagccgatg atcgtcttca cgccgaagtc gatgatgcga
     9901 cgcaaagacg cagcatcgca gccggacgac ttcacgtccg gcacgttcgc cccggtcatc
     9961 ggtgacgacg tggacgatcc gaacgcggtg gagcggctgc tgctgtgctc cgggcggatc
    10021 tgctgggatc tgctcgccga gcggaagcgg cgcgaggacg gtcagtcacg gacggcgatc
    10081 gcccggctgg agcagctgta cccgagacct gtcgacgaag tgcaggcgga gctggcgaag
    10141 tacccgaacc tgcgggagat tcgttgggtc caggacgagc cggcgaacat gggaccctgg
    10201 ccgcacgtga agctcaacct ggcgccggaa ctgggcgacg tcccgttcca gcggatctcc
    10261 cgtccggagt cggcggcgcc ctcggtcggt cagagcgccg tacaccagca ggaactgcag
    10321 aacatcctcg acgagacgtt cgcctgaccc gtcggctctg cctgaggatc gcatgtactt
    10381 cactgatcgt ggcatcgaag agctcaccga ccgccgcggt gacgaagagg tcacgctggc
    10441 ctggcttgcc gagcggctgt cggagttcgt cgacctgaac ccggagttcg agacccccgt
    10501 cgagcggctg gccacctggc tcgctcgtct cgacaacgaa gacgacctcg attgaccgct
    10561 gcggtttgag gtttgcgatg tttacagccc aaaaggcgtg taaacatcgc aaaccctcga
    10621 tgggcacagt cggtctcgga tgtgaacatc gcgaaccgca gggtcggccc gacagccgat
    10681 ggccgaaacc gccagtcaca cacccaccga acggaacgga aagccggccg cctccgaaaa
    10741 accacctacg ccaccaagcg gacatacgtc gggtggtggt ttgcctcggt agccatgcaa
    10801 acagaccggt agccaggtag atctgccttg gcaggggtgg gcttgcatgg gtagccatgc
    10861 aaaccgctgc tcggcgagca cggatgtccg aaaaccgcca ggcacacacc cgccaaaagg
    10921 gaacggaacg ccggccggcc accccaaggg tcacgaacgc atccgctctt caaagccgac
    10981 ccacctcagc ccatcgcgac ggaacgccgg ccggcccccc cagg
//
LOCUS       DATBFZ010000110         4966 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_398087, whole genome shotgun sequence.
ACCESSION   DATBFZ010000110 DATBFZ010000000
VERSION     DATBFZ010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 4966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4966
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398087"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..853)
                     /locus_tag="VH419_03355"
     CDS             complement(<1..853)
                     /locus_tag="VH419_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002538950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEY7042684.1"
                     /translation="MTANKREYADEPDFSDEGPDSSEIELHDDISVDRLDLESADPDD
                     AHDWGANGEDAFGDSQGDLDLEERYALRRVAGLSTELEDITEVEYRQLRLERVVLVGV
                     WTEGTLEDADNSLAELKLLAETAGSQVLDALSQRRQKPDPSTYIGPGKVDHLTAVVAE
                     TGADTVICDGELSPSQLRNLEDKVRVKVVDRTALILDIFAQHARSKEGKAQVELAQLE
                     YLRQRLRGWGGNLSRQAGGRVGAQGGGIGGRGPGETKLETDRRRINTKIARLRRELAQ
                     MKGTRDTK"
     gene            complement(1069..1911)
                     /gene="dapF"
                     /locus_tag="VH419_03360"
     CDS             complement(1069..1911)
                     /gene="dapF"
                     /locus_tag="VH419_03360"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008362567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEY7042685.1"
                     /translation="MSFPFVKGHGTRNDFVLLPDLDGTVHGDLEPDIVVALCDRRAGI
                     GADGVLRVLRGDVDGGAEWFMDFRNADGSVSEMCGNGLRVFARYLADAGLVDVSAMDG
                     PLPVDTRDGIKLVTFCADEEISVDMGNPKVGDPVVVSLGGVARDGRRVDIGNPHAVVF
                     VDDLADAGDLAHSPYFCSAEYPGGVNVEFVVRHRPNHVAMRVFERGVGETCSCGTGAC
                     AAVAAVVVADAESSTGTGRAPDLPATYAVDVPGGRLTVTWNTDDHIHLKGPAVLVASG
                     TWSV"
     gene            complement(1962..2882)
                     /gene="miaA"
                     /locus_tag="VH419_03365"
     CDS             complement(1962..2882)
                     /gene="miaA"
                     /locus_tag="VH419_03365"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012922292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEY7042686.1"
                     /translation="MLPPVVAVVGPTAAGKSDLAVALAEALGGEVVNGDAMQVYRGMD
                     IGTAKLAAHERRGVPHHLLDVLDVTEPATVAEFQGWARAAIRDCRDRGVVPIIAGGSA
                     LYLRAVLDVFEFPGTDPEVRARLEAELATVGPAVMHERLGAVDPAAAAVILPGNGRRI
                     VRALEVVELTGGPFRATLPDHTYAFDRVVQVGLDVPRDVLDRRIEARVERMWAAGLVD
                     EVRRLESLGLRRGRTASRALGYAQVLRFLAGECSEDEARAETVRGTRKFARRQDTWFR
                     RDPRIHWLPFDAPAPHLLAQVLDLIPTVEP"
     gene            complement(2960..3334)
                     /locus_tag="VH419_03370"
     CDS             complement(2960..3334)
                     /locus_tag="VH419_03370"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HEY7042687.1"
                     /translation="MDRDRWLLPRLRSEATEAFDWLVKHAGPYPFSSLGIVVVGGQSA
                     METQTMITASRDILDVPGANLEHEMAHQWFGDSVSPVDWRGLWLNEGWAMYMQQWYES
                     DTGRPGWGGGMRAWPLRPGQLR"
     gene            complement(3334..4020)
                     /locus_tag="VH419_03375"
     CDS             complement(3334..4020)
                     /locus_tag="VH419_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042688.1"
                     /translation="MRWVLQAGSAAGRAAVQRWPAPIAATAVVAAALTGCSGSSDAPD
                     SSDALRTPSTSSAVPEQPGPDGATAADGSADGEVPGAAALQAGRSTPVEDPYYPETSN
                     PEVDVLHYLLDLTYDGSGTLDGRAAVTFRVTSVTDTVRLDLSPALDVDAVTLDGEPIG
                     FDHSGDGLVMSTAGLEPGATHAFVISYAGTPQPTEAPSRRRRTWQRSPSVPTTRTVPG
                     PALASRCRCG"
     gene            4069..4287
                     /locus_tag="VH419_03380"
     CDS             4069..4287
                     /locus_tag="VH419_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020391270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEY7042689.1"
                     /translation="MGWLDRFKKKTADTVDQHGEQVSTGLDKSADVVDEKTGGKHTEK
                     IESAKVKAEDTLDNLDGKNDDIPDSPAR"
     gene            complement(4332..>4966)
                     /locus_tag="VH419_03385"
     CDS             complement(4332..>4966)
                     /locus_tag="VH419_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042690.1"
                     /translation="AACLAAVADLVAAAVDRLVVVTDADEDGEWSGTARGDLSGYGCS
                     AVFGGDDADGTGLPPPLTIGAYLLDAAGVPVQRRRYLAHASTTSPADCAAIGRALVGA
                     EHRIGLLVLADGSAKRTTSSPGYLDRRAVDYDADVVRALLDVDLDALDALDPALAAEL
                     WVAGRPGWQVLSGAGRQALADGASISTRATYDAAPYGVGYAVVEWLLSYP"
BASE COUNT          796 a         1746 c         1689 g          735 t
ORIGIN      
        1 gcttggtgtc gcgggtgccc ttcatctgcg cgagctcgcg acgtagccgg gcgatcttgg
       61 tgttgatgcg ccggcggtcg gtctcgagct tggtctcgcc gggaccgcgg ccaccgatgc
      121 cgccgccctg cgcgcccacg cgaccaccgg cctgccggga caggtttcca ccccagccgc
      181 gaagtcgctg tcggaggtac tcgagctgcg cgagctccac ctgcgccttg ccctccttcg
      241 agcgggcatg ctgggcgaag atgtcgagga tcaacgcggt ccggtcgacg accttgaccc
      301 tcaccttgtc ctcgaggttg cgcagctggg aggggctgag ctcgccgtcg cagatcacgg
      361 tgtcggcccc ggtctcggcc accacggcgg tgagatggtc gaccttgcct gggccgatgt
      421 acgtcgacgg gtccggcttc tgccgacgct gcgagagtgc gtcgagtacc tgggaaccgg
      481 ctgtctcggc gagcagcttc agctcggcca acgagttgtc tgcatcttcc agcgtgccct
      541 cggtccacac gccgaccagc accacccgct cgaggcggag ctggcggtac tcgacctcgg
      601 tgatgtcctc gagctcggtc gacaggccgg ccacccgacg caaggcgtag cgctcctcga
      661 ggtcgaggtc gccttggctg tcgccgaacg cgtcttcacc gttggcaccc cagtcgtgcg
      721 catcgtcggg gtcggcggac tcgagatcga ggcgatcgac ggagatgtcg tcgtggagct
      781 cgatctcgct gctgtcgggt ccctcgtccg agaagtcggg ctcgtcggca tactcacgtt
      841 tgttcgcagt catcgtgcct ccactgtacg gcgcggggac gccctgccac gactcgatta
      901 cgcgacggct gcgccttcac tcttcaacca ctgcccgggg tcctggggtg cggtttgcga
      961 tgtttacatg ccaggcgcac cctttacatc gcggatctgc cgagtagaca gcccgcttaa
     1021 gctgtttaca tcgcaaacca caccgggcac tgaagaagac gatgtgcctc agacggacca
     1081 tgttccggag gcgaccagga cagccggacc cttgaggtgg atgtggtcgt cggtgttcca
     1141 tgtcacggtg agccggccac cggggacgtc gacggcgtac gtcgcgggca ggtccggggc
     1201 tcggccggtt ccagtactcg actcggcatc ggcgacaacc acagcagcca ctgcagcgca
     1261 ggcgccggtc ccgcacgagc acgtctcccc cacgccgcgt tcgaacaccc gcatcgccac
     1321 gtggttcggg cggtgccgga ccacgaactc cacgttgacc cccccgggat actcggcgct
     1381 gcagaagtag ggggaatgcg cgaggtcccc ggcgtccgcg aggtcgtcga cgaagacgac
     1441 cgcgtgcggg ttcccgatgt ccacccgccg cccgtcacga gccacaccgc cgagcgatac
     1501 gacgaccggg tcgccgacct tggggttgcc catgtcgacg ctgatctcct cgtcagcgca
     1561 gaacgtgacc aacttgatgc cgtctcgggt gtccaccggc aacgggccgt ccatcgcgga
     1621 gacgtcgaca agtccggcgt cggcgaggta gcgggcgaag acgcgcaacc cgtttccgca
     1681 catctccgac actgaaccgt cggcgttgcg gaagtccatg aaccactccg ctcccccgtc
     1741 gacgtctcca cgtaggacgc ggagcacgcc atcggcgccg atcccggcgc gccggtcaca
     1801 cagcgccacc acgatgtcgg gctcgagatc gccgtgcacg gtcccgtcga ggtccggtag
     1861 caggacgaag tcgttccgcg tgccgtggcc cttgacgaac gggaagctca cgtcaccagc
     1921 ctacggctgc aaccacaact cacggctcga cgaaccacaa ctcacggttc aacggtgggg
     1981 atgaggtcga ggacttgggc gagaaggtgg ggggcggggg catcgaacgg cagccaatga
     2041 atgcgcgggt cacgacggaa ccaggtgtcc tggcgacgtg cgaacttccg cgttccgcga
     2101 accgtctccg cacgcgcctc gtcctcgctg cactcgccgg cgaggaagcg cagcacctgc
     2161 gcatacccga gtgcccggct ggcggtccgc ccgcgtcgca atccgagcga ctccagccgg
     2221 cgtacctcgt cgaccagccc ggccgcccac atccgttcga cccttgcctc gatccgcctg
     2281 tccagcacgt cgcggggcac gtccaggccg acctgcacga cccggtcgaa cgcgtaagtg
     2341 tgatcaggca gcgtcgcccg gaacgggccg ccggtcagct cgacgacctc cagggcgcgg
     2401 acgatccgac ggccgttgcc cggcaggatc accgcagccg cggctgggtc gaccgcaccg
     2461 agccgctcgt gcatgacagc aggcccgacc gtggcgagct cggcctccag ccgcgcacgt
     2521 acctccggat cggtcccggg gaactcgaac acgtcgagca cggctcgcag gtacagggcc
     2581 gagcctcccg cgatgatcgg tacgacgcct cggtctcggc agtcgcggat cgccgctcgc
     2641 gcccagccct ggaactccgc gaccgtggcg ggctccgtca cgtcgagcac gtccagcaga
     2701 tgatggggta cgccgcggcg ctcgtgtgcc gccaacttcg cggtcccgat gtccatcccg
     2761 cggtagacct gcatggcgtc accgttgacg acttcgccgc ccagcgcctc ggccagcgcg
     2821 actgccaggt cgctcttccc ggcggccgtc ggaccgacga cagcaacaac gggcggcagc
     2881 accggcccat tatccgagtt gtcagaagcc catagggcta tagccccatg gagttctgac
     2941 aagtcggtcg acgttgacgt caccgaagct gtccgggtcg tagtggccag gcccgcatgc
     3001 caccacccca cccaggtcgt ccggtgtcgg actcgtacca ctgctgcatg tacatcgccc
     3061 atccctcgtt gagccacagg ccccgccagt ccaccggcga gacgctgtcg ccgaaccact
     3121 ggtgagccat ctcgtgctcg aggttggcac ccggaacgtc gaggatgtcg cggctggccg
     3181 tgatcatcgt ctgcgtctcc atcgcgctct gtccgccgac gacgacgatc cccaggctcg
     3241 agaaggggta cggtccggcg tgcttcacca accagtcgaa cgcctccgtg gcctccgacc
     3301 tgaggcgggg cagcagccac cgatcgcggt ccatcagcca cagcgacatc gtgaggccag
     3361 cgcgggtccg ggcacggtac gcgtggtagg gaccgatggc gatcgttgcc aggtacgacg
     3421 ccgtcggctc ggtgcctcgg tcggctgcgg cgttcctgca tagctgatga cgaacgcgtg
     3481 agtggcgccg ggttcgaggc cggctgtgct catgacgagg ccgtcgccgc tgtggtcgaa
     3541 gccgatcggc tcgccgtcca gcgtcacggc gtccacgtcg agggcggggg acaggtcgag
     3601 ccggaccgtg tcggtgacgc tggtgactcg gaacgtcacg gcggcacgac cgtcgagcgt
     3661 ccccgacccg tcgtaggtga ggtcgaggag gtagtgcagg acgtcgacct cgggattgct
     3721 ggtctcgggg tagtacgggt cctcgacggg ggtggaacgg ccggcctgaa gcgcggccgc
     3781 gcccggaacc tccccgtcgg ccgacccgtc tgctgcggtt gcaccgtccg ggcccggctg
     3841 ttccggtacg gcgctggacg tcgacggcgt acgcagcgcg tcggacgagt cgggcgcgtc
     3901 ggacgaaccg gagcaaccgg tgagtgcggc cgccaccacc gccgtcgcgg cgatcggggc
     3961 cggccagcgc tggaccgccg cacggccggc agccgatccg gcttggagaa cccagcgcac
     4021 gggacaagaa tggtaggaca gcacgtcacg ccccccgagg aggcagcgat gggatggctg
     4081 gacaggttca agaagaagac ggccgacacg gtcgaccagc acggcgaaca ggtcagcacc
     4141 gggctcgaca agtcagctga cgtggtcgac gagaagaccg gcggcaagca caccgagaag
     4201 atcgagtcag ccaaggtcaa ggccgaggac acgctcgaca acctcgatgg gaagaacgac
     4261 gacatcccgg actcgccggc tcgttagcgg tcgagtcccg gcgggtgccg ctcacccctg
     4321 ggatcacgcg gctacggata gctgagcagc cattcgacaa cggcgtagcc gacgccgtac
     4381 ggcgccgcgt cgtacgtcgc cctcgtactg atgctcgcgc cgtccgccag ggcctggcgg
     4441 ccggcgccgg acagcacctg ccagccgggg cgtccggcga cccagagctc agcagccagc
     4501 gccgggtcga gcgcgtccag cgcatcgagg tccacgtcga gcagcgcgcg gacgacgtcg
     4561 gcgtcgtagt cgaccgcgcg ccgatcgagg tacccgggag acgacgtcgt ccgcttggca
     4621 gagccgtctg ccaggacgag taagccgatg cggtgctccg cgccgaccag ggcgcgaccg
     4681 atggcagcgc agtcggccgg gctcgtggtg gacgcatgtg cgaggtagcg ccgacgctgg
     4741 accgggacgc cggcggcgtc cagcaggtag gcgccgatcg tgagcggcgg cggcagccca
     4801 gtgccgtcgg cgtcatcgcc gccgaacacg gcactgcagc cgtatccgct gaggtcgccg
     4861 cgcgccgttc ccgaccactc gccatcctcg tcggcatcgg tgacgacgac cagccgatcc
     4921 accgcggccg cgaccaggtc ggcgaccgcg gccaggcacg ccgccc
//
LOCUS       DATBFZ010000111        10055 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_1380933, whole genome shotgun sequence.
ACCESSION   DATBFZ010000111 DATBFZ010000000
VERSION     DATBFZ010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 10055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10055
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1380933"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="VH419_03390"
     CDS             <1..369
                     /locus_tag="VH419_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY7042691.1"
                     /translation="DSFVAMHERWRRLRDPERALGYLRRSIVNRSRSAHRHHLVVAKH
                     VPERPEDAASAEAHVLTAQTRALVLEALHRLPTRQREVLVLRYYSDLSEAQIAEALGI
                     SSGSVKSHAHRGLAALRTTL"
     gene            378..1373
                     /locus_tag="VH419_03395"
     CDS             378..1373
                     /locus_tag="VH419_03395"
                     /inference="COORDINATES: protein motif:HMM:NF022114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gmad2 immunoglobulin-like domain-containing
                     protein"
                     /protein_id="HEY7042692.1"
                     /translation="MSTDHPGPPDPAAELLTQALREEADTIDTDPVALQSIRQRVSAT
                     KSRRPWVYAGIGAAAATAAVIAGISVVANHSDQGSKGPEPAVTPSPTTVTQVQPVQRA
                     VQVYYVGSSTHRLYVETREVTLHGSAAASAVEAFLSSLPADPDYVNGWPSGVHAVDVA
                     STDAETTITLKGPSDVRFEADPALGPDGGRLALDALLRTAGVPPGAKASFVYNETKLD
                     SLFGVDLPVTVESDDQSRALISIDNIVDGQTVSNPVSVEVSGNTFEGTVNWQLLDDGG
                     AKLDQGFVTTSMGQWAQATIDLGTLDPGTYTIRCLEYSPENGKPGNIDDKTFTVD"
     gene            complement(1424..2155)
                     /locus_tag="VH419_03400"
     CDS             complement(1424..2155)
                     /locus_tag="VH419_03400"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEY7042693.1"
                     /translation="MTAHVFLVDSERLRADPITLDGDEGRHAVVVKRIRVGERVVLTD
                     GRGEAAECRVTSVTRQGLSAEVLVRRSQARAVPRLVVVQAIPKGEHAERAVDLLTEVG
                     ADVIVPWAASRNVVTWKGDRVGKALHRWRATAVSAAKQSRRFWLPDVRDLHSTAEVAG
                     LVAGAGTAFVLHAAATQPLSGVRVPSDGDVVLVIGPEGGITEEELATLTAAGAQLVRL
                     GSEVLRSSSAGMAAAAVILSQTRWA"
     gene            complement(2160..3323)
                     /gene="dnaJ"
                     /locus_tag="VH419_03405"
     CDS             complement(2160..3323)
                     /gene="dnaJ"
                     /locus_tag="VH419_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007078272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEY7042694.1"
                     /translation="MSHDLYDVLGVSRDASPEDIKKAYRRLARQLHPDINPDAETQER
                     FKQVTHAYDVLSDPKKREMYDLGADPLGAAGAQGFGAGFSFTDIMDAFFGQTAASRGP
                     RPRTRRGQDALIRLQLELAEAAFGVSKELKVDTAVTCTLCHGAGTSPGTSPATCSTCG
                     GRGEISHVQRSFLGEVRTMRPCPTCRGFGTVIPNPCSECSGDGRVRARRALNVKIPAG
                     VDSGTRVQLAGEGEVGPGGGPSGDLYVEVDVVQHPVFTRRGDDLLCTITLPMTAAALG
                     TSVELTTLEADLVDPEESDVEQSISLEIRPGTQSGEQLVMRGRGVPRLRGTGRGDLIT
                     TVIVETPTKLDAEQTALVQRLAAIRDEENPVAAVEENRHRSVFGRFRDAFRAP"
     gene            complement(3399..4421)
                     /gene="hrcA"
                     /locus_tag="VH419_03410"
     CDS             complement(3399..4421)
                     /gene="hrcA"
                     /locus_tag="VH419_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012920044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEY7042695.1"
                     /translation="MLDDRKLDVLRAIVEDYVQTQEPVGSKALVERHNLGVSPATVRN
                     DMAVLEEEGFIAQPHTSAGRIPTDKGYRLFVDKLTTIKPLSGAEKRAIATFLQGAVDL
                     DDVVHRSVRLLAQLTQQVAIVQYPTLSRSTVRHVEVVTLTSSRLLLVLITSTGRVEQR
                     VVELAKHLDDDVVGELRARLNTAVVGQRLTDAATRVASLPEAFHRDDKAMVTQVVAAF
                     AEAVADDRSDEKVAIGGTANLVRFGEDFDRSIKPLLEALEEHVILLRLLGEATSPSLV
                     TVRIGHEVPHAQLAETSVVASGYGPAQQPLATLGVVGPTRMDYPGSMAAVHAVARYVS
                     QILSDA"
     gene            4575..5450
                     /locus_tag="VH419_03415"
     CDS             4575..5450
                     /locus_tag="VH419_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020393318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY7042696.1"
                     /translation="MAVQTSYEHGRGFVEVADRVFVGRYPQWDTTVGLVVGADGLLVV
                     DTRACRRHGVELCDDVRRVAPGLPVRWVVNTHEHFDHVMGNAAFEAATIHAHENAAAR
                     MVEACDRIKADIRADPSLDPDHPWYTSELLEAVLETEYRLPDETFSSAAAIDLGDRVV
                     ELAYPGRGHTSGDIAIRVPDVDVVFAGDLIEQSAPPSYGGDCFPLDWPATLDLVIGML
                     TPDTVVVPGHGTPVDKAFVQDQRADVSDIAELIRSLHGQGVPEADALAAGGDGWPWES
                     SRLTHAVSRGYAHLR"
     gene            complement(5474..6730)
                     /gene="hemW"
                     /locus_tag="VH419_03420"
     CDS             complement(5474..6730)
                     /gene="hemW"
                     /locus_tag="VH419_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020388514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HEY7042697.1"
                     /translation="MPSALPDGAPSPADGALPASALGEIGTTPFGFYVHVPFCTVRCG
                     YCDFNTYTASELGDVRGASRASYAEAAIDEVRFARKVLGDIDVPVETIFFGGGTPTLL
                     PPADLVSVVDAVRAEFGLAPDAEITAESNPDSVSPADLCALRDGGVNRISFGMQSSVR
                     SVLAVLDRTHDPERVPDVMGWARTAGFDQVSLDLIYGTPGESVEQWQESVSAAVVLGP
                     NHISAYALIVEDGTALARRIARGELAMTDDDDLADKYEVADDLLAAAGYGWYEVSNWA
                     RSPSVWCRHNLGYWTGADWWGVGPGAHSHIGGVRWWNVKHPTPYAERIAAGVSPAAAR
                     EVLDAETRRVERVLLETRLVTGLPLDVLDAAGRAVVPHLITDGLAELSEPRGAMTRPT
                     SDNSVRLVLTRRGRLLADAVVRSLLP"
     gene            complement(6762..8582)
                     /locus_tag="VH419_03425"
     CDS             complement(6762..8582)
                     /locus_tag="VH419_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008363692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEY7042698.1"
                     /translation="MVESADASALIDNRPASVGQMFFDRVAATPQREAYRRPSAGGWE
                     SVTWEEAGDRVTRLAAGLIALGVEPEQRVAIASSTRYDWILADLAIMSAGGATTTVYP
                     SSNSEDVAYIVGDSDSRLVFAEDDAQLAKLREHRAELATVSKVVTFDGAADGDWVITL
                     DDLEQLGSDLLSEQPDVVTNRVEKTSADSLATLIYTSGTTGRPKGVRLRHSAWTYEGV
                     AVQAEGFLDVDDLQFLWLPLAHSFGKVLLTTQLAIGFPTAVDGTVEKIVDNLAVVRPT
                     FMGAAPRIFEKAHARINTLAADEGGIRKRIFDRAFKVGIDVSRRKRAGQSVPPHLAAQ
                     HALFDRLVFAKIRARFGGRVRFFVSGAAALNRDIAEWFHAAGLLILEGYGLTESAAGT
                     TVNHPDNFKIGTVGLPFGGTEAKIADDGELLIKGPGVMDGYHNNTQATQAALDDGWLH
                     TGDIGEIDEAGFVRITDRKKDLFKTSGGKYVAPQTIETQFKAVCPYASQFVVHGNERN
                     FVSALVTLDPEAIEGWAAQHGLGGRSYAEIVSSDAARSMVQGYVDELNTKLNRWETVK
                     KFVILQQDLSIEGGEITPSMKVKRKVVEDHYQSELDSMYR"
     gene            complement(8648..9319)
                     /locus_tag="VH419_03430"
     CDS             complement(8648..9319)
                     /locus_tag="VH419_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017935614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HEY7042699.1"
                     /translation="MTARLLSINVGTPFEAEWAGDLRRTAIKKHSVTGPVHVHELGID
                     GDQVADTKHHGGTYQAVYAFAREDIDAWVERLGMPIANGQFGENLTTSGIDVNEALIG
                     EQWRIGTTVVQVADVRIPCNVFKGWMGLSGYDDTGWVKRFTLEGRPGPYLQVVQPGVI
                     TTGDEIEVVHKPDHAITVTTMFRAFTTDRDLLPRLLDAGDDIPPKPRAKAAEYAVRTG
                     AVARP"
     gene            complement(9491..>10055)
                     /locus_tag="VH419_03435"
     CDS             complement(9491..>10055)
                     /locus_tag="VH419_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0019748 - secondary metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEY7042700.1"
                     /translation="VLAACEIIAGLYERIHVTAMPQLRTLTSYIARWTEDLSRLPTTA
                     PIPRRLVEQTLSLGRDFVADPASVGRAIHGDLHFENVLAGEREPWLVIDPKPMSGDPH
                     YEVAPLLWNRWDEVIASGDARQAIRARFHAAVDRAGLDEGRARDWVVVRMIHNALWAI
                     DDDPELADPETSESITTSVTIAKAVQD"
BASE COUNT         1597 a         3488 c         3354 g         1616 t
ORIGIN      
        1 gactcgttcg tcgcgatgca cgagaggtgg cgacggctcc gcgatccaga gcgagccctc
       61 ggctacctcc gccgcagcat cgtcaaccgc tcccgctcgg cccaccgcca tcacctcgtg
      121 gtcgccaagc acgtgcccga acgtccggag gacgccgcca gcgccgaagc acacgtcctg
      181 acggcccaga cccgagccct cgtgctcgag gcgctgcacc gactaccgac ccgtcaacgc
      241 gaggtgctcg tcctccgcta ctacagcgac ctgtccgagg cgcagattgc cgaggcgctc
      301 gggatcagct ctggctcggt caagagtcac gcgcaccgcg gtctggccgc acttcgtacg
      361 acgctgtagg aacagcgatg agcaccgacc accccggccc cccggacccg gcggccgagc
      421 tgttgactca ggcgctgcgc gaggaagcgg acacgatcga caccgatccc gtcgctctgc
      481 agtcgatccg acagcgcgtc agcgcaacga agtcgcggcg cccctgggtg tatgccggca
      541 tcggcgccgc ggccgccacc gctgccgtca tcgccggcat cagcgtcgtg gccaaccact
      601 cagaccaggg ctccaagggg cctgagccgg cggtgacacc ctcgccgacg accgtcacgc
      661 aggtccagcc ggtgcagagg gcggtccagg tgtactacgt cgggtcctcg acgcatcggc
      721 tgtacgtcga gacccgcgag gtgaccctgc acggctccgc agccgcctca gcggtggagg
      781 cgttcctcag ctccctgccg gccgatcccg actacgtcaa tggctggccg tcaggcgtgc
      841 acgccgtgga cgtcgcgtcg accgacgcgg agaccacgat cacgctcaag ggcccgtcgg
      901 acgtccgctt cgaagccgac ccagcgctcg ggcccgacgg gggacgactt gccctcgacg
      961 cactgctacg cacggcgggc gtgccgccgg gcgcgaaggc gtcgttcgtc tacaacgaga
     1021 ccaagctgga ctcattgttc ggcgtcgacc tgccggtcac tgtcgagtcg gacgaccagt
     1081 caagggcgtt gatctcgatc gacaacatcg tcgacgggca gacggtctcc aacccggtgt
     1141 ccgtcgaggt ctccggcaac acgttcgagg gcacggtcaa ctggcagctg ctggacgacg
     1201 gtggcgcgaa gctcgaccag ggattcgtga ccacctcgat gggccagtgg gcgcaggcca
     1261 cgatcgacct cggcaccctc gaccccggca catacacgat ccggtgcctc gagtacagcc
     1321 ccgagaacgg caaacccggc aacatcgacg acaagacctt caccgtcgac tgacttgtca
     1381 gaagtacata gggctatggg tccatgtact tctgacaact cggtcaagcc cagcgggtct
     1441 ggctgaggat cacggcagct gcggccatgc cggccgagga cgaacgcagg acttccgaac
     1501 ccagccggac cagctgggca cccgccgctg tcagcgtcgc cagctcctct tccgtgatgc
     1561 cgccttccgg tccgatcacg agcaccacgt caccgtctga cggcacgcgt acgccggaca
     1621 acggctgcgt cgccgccgca tggagcacga aggcggtgcc ggcgccggcc accagaccgg
     1681 cgacctcggc ggtggaatgc agatcacgca cgtccggaag ccagaaccgc ctcgactgct
     1741 tggccgctga cacggcggtg gcccgccacc gatgcagcgc cttgccgacc cgatcgccct
     1801 tccacgtgac cacgttgcgc gacgcagccc agggcacgat cacgtccgca ccgacctcgg
     1861 tgagcaggtc gacggcgcgt tcggcgtgtt cgcccttcgg gatcgcctgt acgacgacga
     1921 ggcggggtac ggcgcgggcc tgcgagcggc gtacgagcac ttcggcggac aaaccctgcc
     1981 gagtcaccga cgtcacccgg cactcggccg cctcgccgcg accatcggtg agcacgaccc
     2041 gttcaccgac ccggatgcgc ttgacgacaa ccgcatgccg cccttcgtca ccgtcgagcg
     2101 tgatcgggtc agcccgaagg cgttcgctgt cgacgaggaa gacgtgggcg gtcatctggt
     2161 cagggcgcgc ggaacgcgtc gcggaatcgg ccgaagacgc tccggtgacg gttctcctcg
     2221 acggcggcca ccgggttctc ctcgtcccgg atcgccgcca gccgctggac cagcgcggtc
     2281 tgctcggcgt ccagcttcgt cggcgtctcg acgatcacgg tcgtgatcag atcgcctcgc
     2341 ccggtaccgc gcagccgggg tacgccgcga ccgcgcatca ccagctgttc accggactgc
     2401 gtaccaggac ggatctcgag cgagatcgac tgctcgacgt cgctctcctc cgggtcgacc
     2461 agatcggcct cgagcgtcgt gagctcgacc gaggtaccga gggcggccgc tgtcatcggc
     2521 agggtgatcg tgcagagcag gtcgtcgccc cgtcgggtga agaccggatg ctggacgacg
     2581 tcgacctcga cgtacaggtc gcccgacggc ccaccgcccg gacccacctc gccctcgccg
     2641 gccagctgca cgcgcgtgcc gctgtcgacg ccggctggga tcttcacgtt cagagcacgt
     2701 cgtgcccgca cccgaccgtc gcctgagcac tccgagcacg ggttggggat gacggtgccg
     2761 aagccgcgac aggtggggca cggccgcatc gtgcgcacct cgccgaggaa cgaccgctga
     2821 acgtggctga tctcgccccg gccgccgcac gtgctgcagg tggcgggcga cgtacccggc
     2881 gacgtcccgg cgccgtggca cagcgtgcac gtgacggccg tgtcgacctt gagctccttc
     2941 gacaccccga acgccgcctc ggcgagctcg agctgcagcc ggatcaacgc gtcctgtccg
     3001 cgccgggtac gcggacgtgg gccacgactg gccgcggtct gtccgaagaa cgcgtccatg
     3061 atgtcggtga acgagaatcc cgcaccgaac ccctgcgcac ccgcggcacc cagcgggtcg
     3121 gcacccaggt cgtacatttc gcgcttcttc gggtcgctca gcacgtcgta cgcgtgcgtc
     3181 acctgcttga aacgctcctg cgtctcggca tcggggttga tgtcgggatg cagctggcgc
     3241 gccagtctgc ggtaggcctt cttgatgtct tcgggactcg cgtctcgcga gactccgagg
     3301 acgtcataca gatcgtgaga catactccgg caatcgttcg ggtcggatgg acggtcgggc
     3361 ttctgactgg attcggttcg tacgccgcat cgtggtgatc aggcgtcgga gagaatctgg
     3421 ctgacgtaac gggcgacggc gtggacggcg gccatcgaac ccggatagtc catccgggtc
     3481 gggccgacga cgccgagggt ggcgagcggc tgctgagcgg gaccgtagcc gctcgccacg
     3541 accgaggtct ctgcaagctg ggcgtgtggg acctcgtgac cgatccggac cgtcacgaga
     3601 ctcgggctgg tcgcctcacc gagcaggcgc aagaggatga cgtgctcctc cagagcctcg
     3661 agcagcggct tgatcgaacg atcgaagtcc tcaccgaacc ggaccaggtt ggcggtgccg
     3721 ccgatggcga ccttctcgtc cgaccggtcg tcggcgaccg cctcggcgaa cgctgccacg
     3781 acctgcgtca ccatggcctt gtcgtcgcgg tggaacgcct cgggcagcga ggccacccgg
     3841 gtggctgcgt cggtcagtcg ctggccgaca accgcagtgt tgagcctggc gcggagctcg
     3901 ccgacgacgt cgtcgtcgag gtgcttggcc agctcgacga cccgctgctc gacgcggccc
     3961 gtactggtga tgagcaccag cagcagtcgc gagctggtca acgtgacgac ctcgacgtga
     4021 cgcaccgtcg aacgcgacag cgtcgggtac tggacgatcg cgacctgctg ggtgagctgc
     4081 gccagcagtc ggacgctgcg gtgcacgacg tcgtcgaggt cgaccgcgcc ctgcaggaac
     4141 gtggcgatcg cccgcttctc ggcccccgag agcggcttga tcgtggtcag cttgtcgacg
     4201 aacagccggt agcccttgtc cgtggggata cggcccgcgc tcgtgtgggg ctgggcgatg
     4261 aacccctcct cctcgaggac cgccatgtcg ttgcggacgg ttgccggtga cacgccgagg
     4321 ttgtgccgct cgaccagggc tttcgacccg accggctcct gggtctgcac gtagtcctcg
     4381 acgatcgcgc gaaggacgtc gagcttgcgg tcgtcgagca tcccgcctcc ttcgtcgcgt
     4441 cgagcccgtc catcgccggg actcatctgg cactcgtgtt ctgtgagtgc caatcctact
     4501 aggtcgagcg tctcccgccg tcgctccagg accgacgctc ggcttcgtcc gggtgcttcc
     4561 ctaggctggc tcgcatggct gtccagacgt cgtacgagca cggccgtggc ttcgtcgagg
     4621 tggccgatcg cgtcttcgtc ggccgctacc cgcaatggga caccacggtc ggtctcgtcg
     4681 tgggtgcgga tggcctgctc gtggtcgaca cccgcgcgtg ccgacggcac ggggtggagt
     4741 tgtgcgacga cgtacgccgg gtggcgcctg gcctgccggt ccgttgggtg gtcaacacgc
     4801 acgagcactt cgaccacgtg atgggcaacg cggctttcga ggcggccacg atccacgctc
     4861 acgagaacgc tgcagcccgc atggtcgagg cgtgcgaccg gatcaaggcc gacatccggg
     4921 ccgacccctc cctcgacccg gaccacccct ggtacacgag tgagctgctc gaggccgtgc
     4981 tcgagacgga gtaccggctg ccggacgaga cgttctcgtc cgcagccgcc atcgacctcg
     5041 gagaccgtgt cgtcgagctg gcatatccgg gacgcgggca cacgtccggc gacatcgcga
     5101 tccgggtgcc tgacgtcgac gtcgtcttcg cgggtgacct gatcgagcag tccgctccac
     5161 cgtcgtacgg gggtgactgc ttcccgctcg actggcccgc cacgctcgac ctggtcatcg
     5221 ggatgctgac gccggacacg gtcgtcgtac ctggtcatgg cacgccggtg gacaaggcgt
     5281 tcgtgcagga ccaacgtgcc gacgtgtccg acatcgccga gctgatccgc tctctgcatg
     5341 gccagggtgt cccggaggcc gatgcgctcg cggccggtgg tgacggctgg ccgtgggaat
     5401 cctcccgtct tacccatgcc gtctcccgtg gctacgccca ccttcgttga accgtgagtt
     5461 gtggttcgtt gagttagggc aggagagacc gtacgacggc gtcggcgagc aaccggccgc
     5521 gccgcgtcag caccagccgc accgagttgt cagacgtggg tcgagtcata gccccacggg
     5581 gttctgacaa ctcggccaag ccgtcggtga tgaggtgcgg tacgacggct cgcccggcgg
     5641 catcgagtac gtcgagcggc aggcccgtga cgagccgggt ctcgagcaag acgcgttcca
     5701 cgcggcgcgt ctcggcatcc agcacctctc tggcagcggc gggactgacg ccggcggcga
     5761 tgcgttcggc gtacggcgtc gggtgcttca cgttccacca gcgaacaccg ccgatatggg
     5821 agtgagcacc tgggccgacg ccccaccagt cggcgcctgt ccagtagccc aggttgtggc
     5881 ggcaccacac cgacggagag cgggcccagt tcgagacctc gtaccagcca tatccagccg
     5941 ccgccagcag gtcgtcggcg acctcgtact tgtccgcgag gtcgtcgtcg tccgtcatcg
     6001 cgagctcacc gcgcgcgatc cgtcgagcca gcgccgtgcc gtcctcgacg atcaacgcgt
     6061 aggcgctgat gtggttaggg cccagcacca ccgctgccga cacgctctcc tgccactgct
     6121 cgacggactc tcccggcgtg ccgtagatca ggtcgaggct gacctggtcg aagccagccg
     6181 tgcgcgccca acccatcacg tcaggcactc gctccggatc atgcgttcgg tcgagcaccg
     6241 ccagcacgga ccgaaccgac gactgcatgc cgaaggagat ccggttcacg ccgccgtccc
     6301 gcagcgcgca caggtcagca ggactcacac tgtcggggtt cgactccgcg gtgatctcgg
     6361 cgtccggcgc cagcccgaac tcggcgcgga cggcgtcgac caccgaaacc aggtcggccg
     6421 gcggcagcag cgtcggcgtg ccaccaccga agaagatcgt ctcgaccggc acgtcgatgt
     6481 cgccgagcac cttgcgggcg aaccggacct cgtcgatcgc ggcttcggcg tacgacgcgc
     6541 gcgacgcccc acgtacgtcg ccgagctccg aggccgtgta ggtgttgaag tcgcagtaac
     6601 cacatcgcac cgtgcagaac ggcacatgga cgtagaagcc gaagggcgtg gtaccgatct
     6661 cgcccagggc cgaggcagga agcgcgccgt cggccgggga aggcgcaccg tcagggaggg
     6721 ctgaaggcac ccgttcatga tgacatcggc cggacctacg gctaccggta catcgagtcc
     6781 aactcggact ggtagtgatc ctcgacgacc ttacgcttga ccttcatgct cggcgtgatc
     6841 tcgccgcctt cgatcgacag gtcctgttgg aggatcacga acttcttgac cgtctcccac
     6901 cggttgagct tggtgttgag ctcgtcgacg tacccctgca ccatcgagcg ggctgcgtcg
     6961 gaggagacga tctcggcgta cgacctgcca ccgaggccat gctgcgctgc ccatccctcg
     7021 atcgcctcgg ggtcgagcgt caccagggcg gaaacgaagt tccgctcgtt gccgtggacg
     7081 acgaactggc tggcgtacgg gcacaccgcc ttgaactgtg tctcgatcgt ctggggggcc
     7141 acgtacttgc cgccggacgt cttgaagagg tccttcttgc ggtcggtgat ccggacgaag
     7201 cccgcctcgt cgatctcgcc gatgtcgccg gtgtgcagcc acccgtcgtc gagcgccgcc
     7261 tgcgtggcct gggtgttgtt gtgatagccg tccatcaccc cggggccttt gatcagcagt
     7321 tcgccgtcgt cggcgatctt cgcctccgtg ccgccgaacg gcagaccgac cgtcccgatc
     7381 ttgaagttgt cgggatggtt caccgtcgtg ccggccgccg actcggtgag gccgtacccc
     7441 tcgaggatga gcaggccagc agcatggaac cactctgcga tgtcgcggtt gagcgcggcc
     7501 gcgcccgaca cgaagaacct cacccggcca ccgaagcggg cgcgaatctt cgcgaagacg
     7561 agccggtcga acagcgcgtg ttgagcagcc aggtgcggcg gcacactctg accggcgcgc
     7621 ttgcgacgcg acacatcgat gccgaccttg aacgcacggt cgaagatccg cttcctgatc
     7681 ccaccctcgt cggcggccag cgtgttgatc cgcgcgtgcg ccttctcgaa gatcctgggg
     7741 gcggcgccca tgaacgtggg tcgtacgacg gccaggttgt cgacgatctt ctcgacggtg
     7801 ccgtcgaccg cagtcgggaa cccgatggcc agctgtgtcg tcagcaagac cttcccgaag
     7861 gagtgcgcca aggggagcca caggaactgc aggtcgtcga cgtccaggaa gccctcggcc
     7921 tggaccgcca cgccctcgta ggtccacgca gagtgacgca gccgaactcc cttcgggcga
     7981 cccgtcgtac ccgacgtgta gatgagcgtc gcaagactgt ccgccgaggt cttctcgaca
     8041 cggttcgtca cgacgtccgg ctgctctgag agcaggtcgg agccgagctg ctcgagatcg
     8101 tcgagggtga tcacccagtc cccgtcggcc gccccgtcga acgtgacgac cttgctcacg
     8161 gtcgccagct cggcccggtg ttcacggagc ttggcgagct gcgcgtcgtc ctcggcgaag
     8221 acgaggcgcg agtcggagtc gccgacgatg taggcgacgt cctcggagtt gctcgacggg
     8281 tagaccgtgg tcgtggcacc acccgccgac atgatcgcga ggtcggccag gatccagtcg
     8341 tagcgggtgc tcgaggcgat cgcgacccgt tgctccggct ccacaccgag ggcgatgagc
     8401 ccggcggcca gtcgggtgac ccggtcgccc gcctcctccc acgtgaccga ctcccagcca
     8461 ccagcggagg gacgacggta ggcctctcgc tgcggcgtcg cagccacccg gtcgaagaac
     8521 atctgtccga ccgaggccgg ccgattgtcg atcagtgcag aggcatctgc ggactccacc
     8581 atggagagaa ccgtagaaca gccatgcacg aatcgggtac ttccggtggc gtcgcagcga
     8641 cgtcgcgtca gggcctggcg acggcaccgg tccggacggc gtactccgcg gccttggcac
     8701 gaggcttcgg cggtatgtcg tctccggcgt ccagcagtcg aggcagcagg tcacggtcgg
     8761 tcgtgaaagc ccggaacatc gtggtgacgg tgatggcgtg gtcgggcttg tgcacgacct
     8821 cgatctcgtc accggtcgtg atgacgccag gctggacgac ctggaggtac gggccgggtc
     8881 gaccctcgag ggtgaagcgc ttgacccagc cggtgtcgtc gtaccccgac agccccatcc
     8941 agcccttgaa gacgttgcac gggatgcgga cgtccgcgac ctggaccacg gtggtcccga
     9001 tgcgccactg ctcaccgatc aacgcttcgt tgacgtcgat gccggacgtg gtgaggtttt
     9061 ccccgaactg gccgttcgcg atcggcatcc cgagccgctc cacccaggca tcgatgtcct
     9121 cgcgggcgaa cgcgtagacg gcttggtacg tgccaccgtg gtgcttcgtg tcggcgacct
     9181 ggtcgccgtc gatcccgagc tcgtggacgt ggacgggacc ggtcaccgag tgcttcttga
     9241 ttgcggtacg ccgcaggtct ccagcccatt cggcctcgaa cggagtaccg acgttgatcg
     9301 agaggagacg cgccgtcatg gcgtccatcc tgacaaaccc cagcaccttc ccgcgaacgc
     9361 attgcaccat gcggcacctg ccgggctccc cggaattgcg ggaaagtggc cgtcgacacg
     9421 ccagcccgag gggcactttt cccgcaattc gcggagaccc caaggcccgg catgacagcg
     9481 ctttgcggca tcagtcctgg accgccttcg cgatggtgac cgaggtggtg atcgactcgc
     9541 tcgtctcggg gtcggcgagt tcagggtcgt cgtcgatcgc ccagagtgcg ttgtgaatca
     9601 tccggacgac cacccagtcg cgggcccggc cctcgtcgag cccggccctg tcgacggcgg
     9661 cgtggaaccg cgcccggatc gcctgccgtg cgtcgccgga ggcgatcacc tcgtcccagc
     9721 ggttccacag gagtggggcg acctcgtagt gcgggtctcc cgacatcggc ttggggtcga
     9781 tgaccagcca cggctcgcgt tcccccgcca gcacgttctc gaagtgcagg tcgccgtgga
     9841 tggcccggcc cacgctggcc gggtcggcga cgaagtcacg tcccagcgag agcgtctgct
     9901 ccaccagtcg cctcgggatc ggggctgtcg tcggcagtcg gctgaggtcc tcggtccagc
     9961 gcgcgatgta cgacgtcagc gtcctgagtt ggggcatcgc ggtgacgtga atgcgctcgt
    10021 agagaccggc gatgatctcg caggccgcca ggacc
//
LOCUS       DATBFZ010000112         6184 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_725638, whole genome shotgun sequence.
ACCESSION   DATBFZ010000112 DATBFZ010000000
VERSION     DATBFZ010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 6184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6184
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_725638"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /locus_tag="VH419_03440"
     CDS             complement(<1..448)
                     /locus_tag="VH419_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042701.1"
                     /translation="MPYSVAEAEALRRFIKQDLLASVLSAALLLGLAVVLSEPWFIAV
                     SAAAAGVAVSLVFARRLTTTDPAKAVHVIADANWLCAVVVTAIVPVALDITPLIVLMP
                     LVLAIPYLPRRAFAGLIGAGVVVSVVLAMVGRLQEGAGLEAQAADWL"
     gene            complement(448..852)
                     /locus_tag="VH419_03445"
     CDS             complement(448..852)
                     /locus_tag="VH419_03445"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY7042702.1"
                     /translation="MRRGPVRVLVVDDQSTFREAASATIAVTPDFVLAAEATSGEAAL
                     ALVAQGDVDLVLMDVNMPGIGGVTAARELRRAHPGLAVVLLSTYDPDCLPEEILRSGV
                     RYLHKERFGPAALGRLAHDLLSGQSSDHADSP"
     gene            complement(910..1575)
                     /locus_tag="VH419_03450"
     CDS             complement(910..1575)
                     /locus_tag="VH419_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014057620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY7042703.1"
                     /translation="MVRVVVAEDNVLVRTGIETLLEPVADIDVVRACSTYDELMRAIE
                     EHQPDVVLTDIRMPPSGTDEGIRAAVRLRTTHPGVGVVVLSQYLDPVQALTLVEDGSR
                     GRGYLLKDRVADPGQLTAAIRAVAGGGSFIDSAVVDALISARAGTGSGRLARLTPREV
                     EILGEIAAGRSNAAIAAALGVTDRAVEKHINSIFSKLGLTEDDTGVHRRVQAVLVFLG
                     ATN"
     gene            2703..3647
                     /locus_tag="VH419_03455"
     CDS             2703..3647
                     /locus_tag="VH419_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042704.1"
                     /translation="MNVNTVFEPAVPEVQQIVSERRSDGATVVRMLKAVVASVTYVSA
                     ILLYCGWAYTQARTSYFGIYSSVLGYPSQEYILRSANVLFRPGLAAALIVLVGVAVNE
                     VASRLRRRSLWSPRSARLYIALRWSLAATGVLLVLVGYGKSFGGFIPSQRLWEGAAAL
                     LAGTALCGYAVLILPPCRGDTVPLLSPPAHRIVRGLILLAICLESFWLVDNYANQLGD
                     DTSRQLAGNLAAVPGVIVYSSHPLGITGPGVRVDTSSSPDGTTAYRYDGLCLLSYWSR
                     RYFLLPVGWDRENGDRAVVLPETDTTRVEFRPACARVS"
     gene            3754..6129
                     /locus_tag="VH419_03460"
     CDS             3754..6129
                     /locus_tag="VH419_03460"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY7042705.1"
                     /translation="MGARRIDRALTEGQQRLDEVLKANLIGDLIAQVAIVIAWFIFPS
                     PWVIVLWLTRWQVIGTIVWARREVDSVVSARPIVINSAGHLVGALTVTTIVPELAPIT
                     LLIIVCDVQLWYEYVSTRPNFTYERVAAVAAGLLSLLSLQKWTGVGQAAPQWLLLALL
                     VGQGSGIALLAARQSWQTLTAARRQGEELAASRKRLQMADDEARRSLTESIAAGPQLE
                     LTRLRTILATSTTRTTSSTREVDSILSRAVGFAESAGSMLREIAHGLHVAELSKQGLA
                     TALSALPWGGSRPGRIEADLRPEPPPDVQHAVADLMNEVASWARLHTSRIEKITARWQ
                     GTDLIFGVQLQNTCGVDFIDVEPRLRDLAGAAGGEVSTHDIGHDMELVGHIPRAGGPP
                     PEAVEVTNTTPSETLESYLRDGLAMTIVGLLVIAAVSAFLRSGQLVILGLVVGLIASI
                     IAVGLAEVRRGFLIRAILLTSLETAIAAIGIAVLLPELSPVLVLITVLPFFVAPSVMS
                     GRQFAELIVVQQLAAAFVLVTYVVHPSSTLGALLPTWLEAAVLFALPIALMGLLTRVY
                     LATILSLEHGNLLLAASRRDIVRVARERRHELERDLHDGAQQIVLALGVQLRVCVRRL
                     ERGDGQESADWRDLLASVDEASEALTIFSSGSYPVALLEHGLAGGIERLARHCPQPTK
                     VRVHVNRGLPAGIELATYYCCSEALQNVAKHAGDNATVDVDIRDAGSRVEFEIADTGR
                     GYLTTTQPGHGLANMKLRVSRLLGDLNITSSPGNGCTVRGWLPLEEPAIVD"
BASE COUNT         1044 a         2154 c         1929 g         1057 t
ORIGIN      
        1 gcagccagtc ggcggcctgc gcttccagac cggcgccctc ctgcagccgg cccaccatcg
       61 ccaacaccac cgacaccacc acccccgccc cgatcaaccc ggcaaatgca cgccgcggca
      121 gatacggaat cgccaggacc agtggcatga gaacaatgag tggtgtgatg tcgagggcga
      181 ccgggacgat ggcggtcacg acgacggcgc acaaccaatt ggcgtcagca atcacgtgga
      241 ctgccttggc gggatcggtg gtcgtgagcc gccgggcgaa tacgagcgag accgccacgc
      301 cggcggcggc cgcactgacg gcgatgaacc aaggctcgga caacaccacg gcgagaccga
      361 ggagcaaggc cgcggagagc accgatgcga gaaggtcctg cttgatgaac ctccgcaggg
      421 cttcagcctc cgcgaccgag tacggcacta gggcgaatcc gcgtgatcgg agctctggcc
      481 ggagagaagg tcgtgcgcca gtcggcccag cgcggccggc ccaaacctct ccttgtgcag
      541 gtagcggact ccgctgcgca agatctcctc tggcaggcag tccgggtcgt acgtcgacaa
      601 caggacgacg gcgaggcctg gatgagcgcg acgcagctcc cgtgccgccg tcaccccacc
      661 gattcctggc atgttcacgt ccatgaggac gaggtccacg tcgccctggg cgaccagtgc
      721 cagcgccgcc tcgccgctgg tcgcctcggc cgccagcacg aaatcaggtg tcacggcgat
      781 cgtggctgac gccgcctcac ggaacgtcga ttgatcgtcg acgacgagta cgcgtactgg
      841 tcccctccgc accggcggat catcgcagag cgggctcgtg gattcgatag tgccagcacc
      901 actgtccggt cagttcgtcg cgccgaggaa aaccaggacg gcctgcacgc gccgatgcac
      961 tcccgtgtcg tcctcggtga gtcccaactt cgagaagatg gagttgatgt gcttctcgac
     1021 ggctcggtcg gtcaccccga gcgcagccgc gatggccgcg ttcgaccggc cagccgcgat
     1081 ttcgcccagg atctcgacct cccgaggcgt caaccgcgcc aaacgtcctg agccggtgcc
     1141 cgctcgggcc gagatcagcg catccacgac ggcggagtcg atgaaggacc cgccgccggc
     1201 cacggcgcgt atggccgccg tgagctgacc cgggtcggca acgcggtcct tcagcaggta
     1261 gccgcgccca cggcttccat cctcgacaag tgtcagagct tgaaccgggt cgagatactg
     1321 cgacaggacg acgacaccga cacccggatg cgtcgtccgc agtcgcacgg cggcacggat
     1381 accttcgtcc gtgccactgg gcggcattcg gatatccgtt aggaccacgt ccggttgatg
     1441 ctcctcgatc gcccgcatca actcgtcata ggtcgagcac gctcgtacga cgtcaatgtc
     1501 ggcgactggc tcgagaagcg tctcgatccc ggtgcggacg agcacgttgt cttcggcgac
     1561 gaccacgcgg accatggcca cggtcgagac cgtactgcgt gggcacacca cggctgctct
     1621 gaatcaccga gcggccggtt cgggtgactc gactcgacgg tggtgtgtcc accaccgaaa
     1681 agaggtgtga cggtacgact gcatgtacct ctttcgcggc gcatcgtgtt cccgttcgtg
     1741 cgacacggat ctcgaccaaa tcgccgagcc tgagatgtca gcgaggccgg ctcgccggtc
     1801 gatctggaag gagaaccatg ccccccaccc tcagtcgagc cgcaggcgtc agtgtgttga
     1861 tgatcactgc actcgcgtcc atcgcggcat gcacgacgcg ccacttgacg cagacacaca
     1921 ccgatcagcc gaccgcgacc acggaaactc tatcgaccac agcatcggga tcttcttcga
     1981 tcgaggtggc tctgccccgc gtcgagacca tgccagtccc ccaagaggtt gtccctcaaa
     2041 ccgacagccg tccaacggga caaccgcaac ctcacgcgtc gggcgccgtg acgtcccagc
     2101 ctggccacga cgtcgccgtg acggcgccga tcatctcagc accgagtcac ggcgtgcacg
     2161 acccgccgcc tggtgcagcc acggccgcgg cgaccactgc gccgccgacg ccggcggagg
     2221 acccgaaccg cccgggaagc aattcggaac ccgtggcctc acctgcccct gccgatgtcg
     2281 cggtggcgcc cggcacgact gatggcgcgc ccgatacgtc gggtggcagt gggaacccga
     2341 agaccgacac gcaaccggct cctggtgacg acgcaccaca cccgccgatg gacggccaac
     2401 cgttcccgcc catcctcgtc tatgtcccac ccgggaaccc ggtgttgccg atcggcccgt
     2461 gcccgtcgcc gatccgcccg atcgagctgt gcgatggcta cggtccgccg agctccgaaa
     2521 ctcccgtgct cactcttccc gccgcgccgc ccggagagac cactcttccc ggcggcccga
     2581 cggaaccgcc gtacagcgtt ccgcccgacc cacatggacc cgtccgaccg cctcatgatg
     2641 gcgacaggaa cctgcctgcg gacggcccgc ctggaagccg tccgtaggac accgaggcca
     2701 gcatgaacgt caacacagtc ttcgagcccg ccgtccccga ggtgcagcag atcgtgtcgg
     2761 aacgtcgcag cgacggggcc accgtcgtcc gcatgctgaa ggcggtcgtc gcctctgtca
     2821 cgtacgtgtc ggcgatcctg ctgtactgcg gctgggccta tacgcaggcc aggacgtcgt
     2881 acttcgggat ctactcttcg gtcctcggct acccgagcca ggagtacatc ctgcggagcg
     2941 ccaacgtgct cttccggcct ggcctcgctg ccgccctcat cgtcctcgtc ggagtagcag
     3001 tcaacgaggt tgcgtctcgt cttcgacgcc gctcgctgtg gtcgccccga tccgcccggc
     3061 tctacatcgc cctccggtgg tcactggcgg cgaccggcgt gctgctcgtc ctcgtcggat
     3121 acgggaagtc gttcggaggc ttcatcccat cgcaacgtct ctgggagggg gccgcagctc
     3181 tcctggctgg caccgccctg tgtggatacg ccgtcctcat ccttccgccc tgtcggggcg
     3241 acaccgtgcc gttgctgtct ccgccggccc accggatcgt gaggggactc atccttctgg
     3301 ccatctgcct ggagtcgttc tggttggtcg acaactacgc caaccagctc ggcgacgaca
     3361 catcacggca gctggccggc aacctggcgg cggttccggg agtgatcgtc tactcctcgc
     3421 acccgctcgg cataacgggc cccggcgttc gcgtagacac ctcttcttca cccgacggca
     3481 caacggcgta ccgctatgac ggcctgtgcc ttctctcgta ttggtcgagg cggtacttcc
     3541 tgctgccggt cggctgggat cgtgagaacg gtgaccgagc ggtcgtactt cccgaaactg
     3601 acacaacacg ggtggagttc cgaccggcgt gtgctcgcgt ttcgtaaggc tcggtcgcgg
     3661 gcccgcacat cgccctgccc cgtcgattgt ccgaagtgat gctccaccat tggtcgacca
     3721 agacggttcc agctcgaatc tacaatcccg aaagtgggcg ctcgccgcat cgaccgcgcg
     3781 ctgacggaag gacaacaacg tctcgacgag gtgctcaagg ccaacctgat cggcgatttg
     3841 atcgcccagg tcgccatcgt catcgcgtgg ttcatcttcc cgtcgccctg ggtcatcgtc
     3901 ctgtggctga cacggtggca agtcatcgga acgattgttt gggcccgccg ggaagtcgac
     3961 tcagtcgtgt cggctcggcc catcgtgatc aactcggccg gccatctcgt cggcgctctc
     4021 actgtcacga caatcgtccc ggagctggcg ccgatcacgc tcctgatcat cgtgtgcgac
     4081 gttcagctgt ggtacgagta cgtctcgacc aggcccaact tcacctacga acgagtggcg
     4141 gcagtggcgg caggcctgct cagcctcctg agcctgcaga agtggacggg cgtcggccaa
     4201 gccgctccgc aatggctcct tctcgctctc ctcgtgggac agggtagtgg catcgcgctc
     4261 ctggcggccc ggcagagttg gcagacgctg accgcggccc gccggcaagg tgaagagctc
     4321 gcggcatccc ggaaacgcct gcagatggcg gatgacgagg cccgtcggtc gttgaccgag
     4381 tcgatcgccg ccggcccgca actggagctg acccgactgc ggacgatctt ggccaccagc
     4441 acgacgcgga cgacctcctc gacccgtgag gtcgactcga ttctctcgcg ggctgtgggt
     4501 ttcgcggagt cggccgggtc catgttgcgc gagatcgcgc acgggcttca cgtcgccgag
     4561 ttgtcgaaac aaggtcttgc cactgcgctc agcgcccttc cgtggggagg cagccggcct
     4621 ggacgcatcg aggccgacct gcggccggag ccgccgcccg atgttcaaca cgccgtcgcc
     4681 gacttgatga acgaggtggc gtcctgggcc cgacttcaca cctcacgcat cgagaagatc
     4741 acagcccggt ggcaagggac ggatctcatc ttcggtgttc agctgcagaa cacgtgcggt
     4801 gtcgacttca tcgacgtcga accgcgcctg cgggaccttg cgggtgccgc aggtggtgaa
     4861 gtgtcgacac acgacatcgg ccacgacatg gagctcgtcg gccacatacc ccgtgccggc
     4921 ggtccaccac cagaagcggt ggaggtcacc aatacgactc cgtccgaaac actcgagtcc
     4981 tacctgcggg acggcctggc catgacgatc gtcgggcttc tggtcatcgc ggcggtctct
     5041 gccttcctgc ggtctggcca gctggtgatc ttgggcctcg tggtcggtct gatcgcatcc
     5101 atcatcgcag tgggccttgc ggaagtacga cgcggcttcc tcattcgtgc gattctcctg
     5161 acgtccctcg aaacggccat cgcagccatc gggatcgctg tactacttcc agagctgagc
     5221 cctgtactcg tgctcatcac cgttctgccg ttcttcgtcg caccgtcggt gatgtctgga
     5281 cgccagttcg ccgagctcat cgttgtccag caattggcgg cagcgttcgt tctcgtgacg
     5341 tacgtcgtgc atccgtcatc cacgctcgga gcccttcttc cgacgtggct cgaagcggcc
     5401 gtcctgttcg cgctgccgat cgcattgatg ggactcctga cacgcgtcta tctggccacc
     5461 atcttgagcc tcgagcacgg caacctgctt ctcgcggcgt ctcgaaggga catcgtgaga
     5521 gtggcgcgag agcggcgcca tgagctcgag cgggacctcc acgacggagc ccagcagatc
     5581 gtgttggcac taggcgtcca gctgcgggtc tgcgttcgcc gcctcgagcg cggggacggc
     5641 caggagtctg ccgactggcg ggaccttctc gccagtgtcg acgaggcgtc cgaggcactg
     5701 acgatcttct cgtccggcag ctatcccgtc gctctgctcg agcacggtct cgccggcggc
     5761 atcgaacgcc tcgccagaca ctgtccgcag ccgaccaagg tacgtgtgca cgtgaaccgt
     5821 ggcctgcccg ccggcatcga gctcgccacc tactactgct gttcggaagc gcttcagaac
     5881 gtggccaagc acgccggcga caacgccacc gtcgatgtcg acatccggga cgccgggagt
     5941 cgtgttgagt tcgagatcgc cgacaccggg agggggtacc tgaccaccac acagccggga
     6001 catgggctcg ccaacatgaa gcttcgtgtg agccggctcc tgggcgacct caacatcacg
     6061 tcgtcaccgg gaaacggatg caccgtccgt ggctggcttc cgctggagga accggcgata
     6121 gtcgactgag tccgacgccg cgaacttgcg atcgctccga ctcactccga gttggcggcg
     6181 agaa
//
LOCUS       DATBFZ010000113         7273 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_6270338, whole genome shotgun sequence.
ACCESSION   DATBFZ010000113 DATBFZ010000000
VERSION     DATBFZ010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 7273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7273
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6270338"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..584)
                     /locus_tag="VH419_03465"
     CDS             complement(<1..584)
                     /locus_tag="VH419_03465"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HEY7042706.1"
                     /translation="MNQEATMHTFATPGPTNLRVELGTGECHVRAEQTDTTTVELVPT
                     HGDSQAQELIDRSTVEQRGDQVAVLVPKGKSSLFGRKGEVTASIVVPTGSAIDVQLGS
                     ADLDTHGVLGRAKVTAGSGDVSLDEVADAEVKTGSGDVRIVTATGSVSAKGGSAEIEI
                     GRVGGRFDFLTGSGDVIVDEAAGPIRGKSGSGDV"
     gene            complement(581..1096)
                     /locus_tag="VH419_03470"
     CDS             complement(581..1096)
                     /locus_tag="VH419_03470"
                     /inference="COORDINATES: protein motif:HMM:NF017355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system HicB family antitoxin"
                     /protein_id="HEY7042707.1"
                     /translation="MDITQFVEALRRDLSQAAEAGTDEMRSAAERLALALDPAVRLTL
                     MDALSEAASEITNELHGTTVEVRLKGREPVFVVLGGAPEAEQPTMAEQEEAFEGESVA
                     RITLRLPESLKTKAEELAARRGQSLNTWLVNAARLASAANFDINITSSMGPGAPGRVR
                     MSNKRIQGWVR"
     gene            1509..3071
                     /locus_tag="VH419_03475"
     CDS             1509..3071
                     /locus_tag="VH419_03475"
                     /inference="COORDINATES: protein motif:HMM:NF024603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEY7042708.1"
                     /translation="MTLRTLNGARTVARRTSSLALGALLTGTLVTLAAPAAQAAAVNN
                     CWNKDNGDPVLTGFSRAPASVDVRHGAKKVNFTVKAHDTGGPGAASGISSVSVFAYSS
                     AGFKSVFATLRKNAAGDYAGAATIPRWTSNGQWQISSVSLSDRAGNYKTYYQTDLSDA
                     GFPTTFSVTSTPDTTAPRLTGFTFSPGSVNTTKAAKSVTFTAKVTDSQSGVNGVYVSA
                     EGKAPNARGFAFLSHVSGPTYRGKMLIGTWVRTSAWHITNVSFSDKIGNFAALSYAQL
                     GARHFKRNLAVKSGIDTGKPTMASFGRAPAKVDVRTKNKQVAVTVRAKDAKSGVASVY
                     VSFVGKTFSTGGFLSRKSGSSHNGVWKGSVLVAKCSASSGRLKGTVTITDLAGNTRDF
                     KVTVLTVRAGDHRVPQATTPSSIATSDPVVVTFNESVNGIDTNSATLRKVGFPNDGPK
                     IAGAWACKTAAGANTNCKTGSVKKATFTPNAALESFTSYNVELNPEHSLGVTDLAGNP
                     FRRYTTGVFTSS"
     gene            complement(3058..3216)
                     /locus_tag="VH419_03480"
     CDS             complement(3058..3216)
                     /locus_tag="VH419_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042709.1"
                     /translation="MISTSSISGFLDTPDQRRPAAAVEPAGTQTPEGVGPDKLDQQVA
                     RAHTLTTT"
     gene            complement(3245..5548)
                     /locus_tag="VH419_03485"
     CDS             complement(3245..5548)
                     /locus_tag="VH419_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020663866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEY7042710.1"
                     /translation="MQVLAVVVSLAITAVAIALFGKAVASIVSVVRTGQPVTGRTDRK
                     TLRWATMLKETLGHTRMLQWSHVGVMHWFVFIGFGFLFFTLVNAFGQLFEADWSLPII
                     GDSVAYAWATDAIAWLMLASILGLILIRVTRLPSGRKGRYSRFFGSRMWQGYFVEAVV
                     VGVGLCILTLRGAEYALADLQGTVSDSHFPLTSFIGESFNNLSRSQLENLIYLVAMLK
                     IVISFTWMIVLALNTTMGVAWHRFTAWPNIWFRREASGRPALGGLQPMMAGGRPIDFE
                     NIDELDEDTVFGVGKVEDFTWKGILDFTSCTECGRCQSQCPAWNTEKPLSPKLLVMGL
                     REHAYTKAEYLLADERTRVGLPATALAEAERPLVGPTDGDPARPSGGAVIDPDVLWSC
                     TSCGACVQQCPVDIEHVDHIMDMRRYQVLVESSFPTELNQLYRGLENKGNPWNMAPNA
                     RMDWAKELPFEVKQVGIDVEDLSEVEWLFWVGCAGAYEDRAKKTTRAVAELLHEAEVS
                     FAVLGDGETCTGDPARRSGNEFVFQQLAMQNAETLEAAKATKVVATCAHCFNTLKHEY
                     RQFGLELDVVHHTQLLNRLVRDGLLTPVAPLNGAARIPGSDVRPRVVTYHDPCYLGRH
                     NRVYEPPRELLAIVPDTEFKEMPRNTERSFCCGAGGARMWMEERLGERINTNRTREAV
                     ATGAQQIAVACPFCRVMLSDGLTSLQADGKARDEVEVVDVAQLLLASVRRTNGTSPPG
                     DPATAAETEATADETESREAVTGDHAE"
     gene            complement(5655..6587)
                     /locus_tag="VH419_03490"
     CDS             complement(5655..6587)
                     /locus_tag="VH419_03490"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY7042711.1"
                     /translation="MAAPGEVVRRMEPQQLEARVVETWDFDDPVGSRAAFEAAVETAG
                     SETPEGMVLRTQVARTHSLQRHFEDADAILDGVQTSFDTQTTAIPEAAAHHVRARIAI
                     ERGRTRNSGGSPGAARPMFETAYDEATAAGLTALAVDALHMSAIAVSATDGPDVAAAV
                     TTRAIAEAEASDDPATRRWLGPLLNNHGWDLHDAGDYEGALKVFRRAVEVRTEFGTPR
                     ELAIAKWAVGRCLRSLGRYVEALEIQEALADSDDAAEDGYVYEEIGENLLALDRGTEA
                     VPYFSQAHDLLSEDDWLVDNESERLERLLTLSDE"
     gene            complement(6704..>7273)
                     /locus_tag="VH419_03495"
     CDS             complement(6704..>7273)
                     /locus_tag="VH419_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HEY7042712.1"
                     /translation="RVDFETRKDWASLQTGGAVDSFDGPDLTPFGCGPEGAIDLSQGR
                     IWGSTTGDDDGTPTNTMTPKHLVVKLARPVDIGTGSGGDSAFKVDPTAGCGDAGSAST
                     GDFTIEVSPNGTTWTTVVDVNGEANWLGRFQYTNVAASQAVAGVQYVRLTLNSPQVPD
                     FATNCPDGAFAGCQFTDFTELEVFGSTTP"
BASE COUNT         1168 a         2438 c         2448 g         1219 t
ORIGIN      
        1 ttcacgtcgc cggaaccgga cttcccccgg atggggccgg cggcctcgtc gacgatcaca
       61 tccccggagc cggtgaggaa gtcgaagcgg ccgccgacgc gaccgatctc gatctcggcg
      121 gaaccgccct tggcggacac cgaaccggtg gcggtgacga tccggacgtc acccgacccg
      181 gtcttgacct cggcgtcggc gacctcgtcc aggctgacgt cgccggatcc ggcggtgacc
      241 ttcgcacggc ccagtacgcc gtgggtgtcg aggtcggccg aaccgagctg gacatcgatc
      301 gctgaacctg tcggtacgac gatggaggca gtcacctcac ctttgcggcc gaagaggctc
      361 gacttgccct tgggtacgag caccgcgacc tggtctccgc gctgctcgac ggtggaccgg
      421 tcgatcaact cctgggcctg actgtcccca tgggtgggaa cgagctccac cgtcgttgtg
      481 tcggtctgct cggcgcggac atggcactcg ccggtgccca gctcgacccg gaggttggtg
      541 ggaccgggtg tggcgaacgt gtgcatcgtg gcctcctggt tcatcgcacc cacccctgga
      601 tgcgcttgtt ggacatgcgg acgcggccgg gggcgccggg tcccatcgag ctggtgatgt
      661 tgatgtcgaa gttcgcggca gacgccagcc gggccgcgtt gaccagccag gtgttcaggc
      721 tctggccacg ccgggcggcg agctcctcgg ccttggtctt cagcgattcg ggcaggcgga
      781 gcgtgatgcg cgccaccgac tcaccttcga aggcctcttc ctgctcggcc atcgtcggct
      841 gttcggcctc cggagcacca ccgaggacga cgaagaccgg ctcgcgaccc ttgaggcgga
      901 cctcgacggt cgtcccgtgg agctcgttgg tgatctccga ggctgcctcc gacaaggcat
      961 ccatcagggt cagccggacg gccgggtcga gcgccagcgc cagccgctcg gcggcgctgc
     1021 gcatctcgtc ggtgccggcc tcggcagctt gggacaggtc tcggcggagc gcctcgacga
     1081 actgggtgat gtccatggcc accaatatga catcactatg gtgtcacgtc aacaccatga
     1141 cgagaaattc tccaaaaggc caggtcagat cacgtgttcg ggctcagtcg ggggctcggg
     1201 gacctcggtg gtcagcagtc gacggtggtg agatgacggc tcacgacgtc atcgcccggt
     1261 ctttgacgtc ataccgatgt cgccgtgacc ccatctggtc acggcgcgtg acgatccgct
     1321 gcggaccgca cccggactac cgctgcgatg gcctgatcgg gccatggcgg cggtggtttg
     1381 gagcaggcac gatccgagtg gacgtcgctc atatcctgac gtggggggac gagggaggaa
     1441 gagtcgtatc cgcggaaagt gtccgttgcg tccgaactgt gtacctgctc gtcaccgaag
     1501 gatgtgacat gaccctgcga actctcaacg gggcgaggac ggtggcgcgc cgcacctcgt
     1561 ccctcgccct cggcgcgctc ctgaccggga cgctggtcac cctggccgca cccgcggccc
     1621 aggcagctgc ggtgaacaac tgctggaaca aggacaacgg tgaccctgtc ctcaccggct
     1681 tctcccgcgc gccggcctcc gtcgacgtcc ggcacggcgc aaagaaggtg aacttcacgg
     1741 tcaaggcgca cgacacgggc ggtccgggag cggccagcgg catcagcagc gtctcggtgt
     1801 tcgcgtactc gagtgccggc ttcaagagcg tcttcgcgac gctgaggaag aacgcagccg
     1861 gcgactatgc cggtgcggcg acgatccccc ggtggacctc gaacgggcag tggcagatct
     1921 cctcggtgtc gctgtcggac cgggccggga actacaagac gtactaccag accgatctga
     1981 gcgacgccgg cttcccgacg accttctcgg tgacgtcgac ccccgacacc acggcgccaa
     2041 ggctgaccgg cttcacgttc tcccccggct cggtgaacac cacgaaggcg gccaagtccg
     2101 tgacgttcac ggcaaaggtc accgactccc agtccggcgt caacggcgtg tacgtcagcg
     2161 cagaagggaa ggcgcccaac gcacggggct tcgccttcct gagccatgtg tcgggaccga
     2221 cgtaccgcgg gaagatgttg atcgggacgt gggttcgcac gtcggcctgg cacatcacca
     2281 acgtctcgtt ctccgacaag atcgggaact tcgccgccct cagctacgcc cagctcggtg
     2341 ccaggcactt caagcggaat ctcgcggtga agagcgggat cgacacgggc aagccgacga
     2401 tggcgtcctt cggacgggcg ccggcgaagg tggacgtccg cacgaagaac aagcaggtgg
     2461 cagtcactgt gcgtgcgaag gacgcgaagt ccggagtcgc ctcggtgtac gtgtcgttcg
     2521 tcggcaagac gttcagcacg ggaggcttcc tgtcccgcaa gtccggctcg agccacaacg
     2581 gtgtctggaa gggcagcgtg ctcgtcgcga agtgttcagc ctcgagcggg aggctcaagg
     2641 gcaccgtcac catcaccgac ctggccggca acacccggga cttcaaggtg accgtcttga
     2701 cggtgcgcgc cggtgaccac cgcgtcccgc aggcgacgac acccagcagc atcgcaacca
     2761 gtgatcccgt tgtcgtgacg ttcaacgagt cggtcaacgg aatcgacacc aacagtgcga
     2821 cgctgcgcaa ggtcggtttc ccgaacgatg gcccgaagat cgccggggcg tgggcgtgca
     2881 agacggcagc tggtgcgaac acgaactgca agacgggcag cgtgaaaaag gcgacgttca
     2941 ctccgaacgc ggctctcgag tcgttcacgt cgtacaacgt ggagctcaac ccggagcact
     3001 cgctcggcgt gaccgacctc gccgggaacc cgttccgccg gtacacgacg ggcgtcttca
     3061 cgtcgtcgtg agggtgtgag cgcgagcaac ctgctggtcg agcttgtcgg gaccaacgcc
     3121 ctccggggtc tgggtcccgg caggctcgac cgccgcagcc ggccgccgct gatcgggcgt
     3181 gtcgaggaac ccgctgatcg agcttgtcga gatcaagggg ccggggtctc gacggccgga
     3241 ggcgctactc ggcgtggtct ccggtgacgg cctcgcgcga ctcggtctcg tcggccgttg
     3301 cttcggtctc cgccgcggtg gcggggtcgc ctggcggtga ggtcccgttc gtacggcgta
     3361 cggacgccag caggagctgc gcgacgtcga ccacctcgac ctcgtccctc gccttgccgt
     3421 cggcctgcag tgaggtcagc ccgtcggaga gcatgacccg gcagaaggga cacgccaccg
     3481 cgatctgctg agcgccggtg gcgaccgctt cgcgcgtgcg gttggtgttg atccgctcgc
     3541 cgagccgctc ctccatccac atccgggccc cgccggcgcc acagcagaac gaccgctcag
     3601 tgttgcgcgg catctccttg aactcggtgt ccgggacgat cgccaacagt tcgcgaggtg
     3661 gctcgtagac acggttgtgg cggccgaggt agcacgggtc gtggtaggtc acgacccgcg
     3721 gccggacgtc gctccccggt atccgtgcag caccgttcag aggtgcaacg ggagtgagca
     3781 ggccgtcgcg cacgagccgg ttgagcagtt gcgtgtggtg gacgacgtcg agctcgagtc
     3841 cgaactggcg gtactcgtgc ttgagtgtgt tgaaacaatg cgcacacgtg gccacgacct
     3901 tcgtggcctt cgccgcctcg agggtctcgg cgttctgcat cgccagctgc tggaagacga
     3961 actcgttgcc cgagcgtcgc gccgggtcgc ccgtgcaggt ctccccgtca ccgagtacgg
     4021 cgaacgacac ctcggcctcg tggagcagct cggcgaccgc ccgggtggtc ttcttcgcgc
     4081 ggtcctcgta ggcgccggca cagccgaccc agaacagcca ctcgacctcg ctcaggtcct
     4141 cgacatcgat cccgacctgc ttgacctcga acggcagctc cttcgcccag tccatccggg
     4201 cgttgggggc catgttccag gggttgccct tgttctccag ccccctgtac agctggttca
     4261 gctcggtcgg gaagcttgac tcgacgagga cctggtatcg ccgcatgtcc atgatgtggt
     4321 cgacgtgctc gatgtcgaca gggcactgct ggacgcaggc cccgcacgac gtacacgacc
     4381 acagcacgtc cgggtcgatg accgcaccgc cggacggtcg cgccgggtcg ccgtcggtcg
     4441 gccccacgag tggacgctcc gcctcggcca gcgcggtcgc ggggaggccg acacgcgtcc
     4501 tctcgtcggc gagcaggtac tccgccttcg tgtacgcgtg ctctcgcagc cccatcacca
     4561 gcagcttcgg cgacagcggc ttctcggtgt tccaggccgg acactgcgac tggcagcgac
     4621 cgcactcggt gcaggaggtg aagtcgagga tgcccttcca ggtgaagtcc tcgaccttcc
     4681 cgacaccgaa gacggtgtct tcgtcgagct cgtcgatgtt ctcgaagtcg atcggtctgc
     4741 cgccggccat catcggctgc agcccgccga gcgccggtcg gcccgacgcc tcgcggcgga
     4801 accagatgtt cggccacgcc gtgaagcggt gccaggccac acccatggtg gtgttgagcg
     4861 cgagcacgat catccaggtg aacgagatga cgatcttgag catcgcgacg agatagatga
     4921 ggttctccag ctggcttcga ctgaggttgt tgaacgactc tccgatgaac gaggtcagtg
     4981 ggaagtggga gtccgacacg gtgccctgga ggtcggccaa cgcgtactcc gcgccacgca
     5041 gggtgaggat gcagagtccg accccgacga cgaccgcctc gacgaagtac ccctgccaca
     5101 tccgcgaacc gaagaagcgg ctgtacctgc ctttgcgccc cgatggaagc cgcgtcaccc
     5161 gaatcaggat cagtccgagg atcgacgcca acatcagcca ggcgatcgcg tcggtggccc
     5221 acgcgtaggc caccgagtcg ccgatgatcg ggaggctcca gtcggcctcg aacagctggc
     5281 cgaacgcgtt gaccagcgtg aagaacagga agccgaaccc gatgaacacg aaccagtgca
     5341 tgacgccgac atggctccac tggagcattc gcgtgtgacc gagcgtctcc ttcagcatcg
     5401 tcgcccagcg cagggtctta cgatcggtgc ggcccgtcac cggctggccg gtccgtacga
     5461 ccgacacgat cgaggcaacg gccttcccga acaaggcgat cgcgaccgcg gtgatcgcca
     5521 gtgagacgac gacggctagc acctgcatga ggtctcctcg ctgttggcgt caccgcaact
     5581 ggcggtcagt gcgtcagcag cctcgaacgt gacgacgatc ctaacgacga accttccgct
     5641 ggtggcggga ggctttactc gtcggacagg gtgagcagcc gttcgagccg ttcgctctcg
     5701 ttgtcgacca gccagtcgtc ctccgacagc aggtcgtgtg cctgggagaa gtacggcacc
     5761 gcctctgtgc cgcggtccag tgcaagcagg ttctcgccga tctcctcgta cacgtagccg
     5821 tcctctgctg cgtcgtcact gtcggcgagc gcctcctgga tctcgagcgc ctcgacgtac
     5881 cgaccgagcg aacgaagaca ccggcctacg gcccacttcg cgatcgcgag ctcccgcggg
     5941 gtgccgaact cggtccgcac ctcgacggcg cgtcggaaga ccttcagcgc cccttcgtag
     6001 tcgccggcgt cgtgcaggtc ccaaccgtgg ttgttcagca acggaccgag ccagcggcgc
     6061 gtggcgggat cgtcggacgc ctcggcctcg gcgatggccc tggtcgtgac ggcggcggcg
     6121 acatcaggtc cgtcggtcgc gctgacggcg atcgcgctca tgtgcaaggc gtcgaccgcc
     6181 agtgccgtca ggcccgcagc ggttgcttcg tcgtacgccg tctcgaacat cggccgtgct
     6241 gcacccggcg agccgccaga gttccgggtt cggccgcgtt cgatggcgat tctggcgcgc
     6301 acatggtggg ccgcggcttc ggggatcgcc gtcgtctgtg tgtcgaagct cgtctggaca
     6361 ccgtcgagga tggcgtcagc gtcttcgaaa tggcgctgca ggctgtgcgt tcgggcaacc
     6421 tgcgtgcgca gcaccatgcc ctcaggggtc tcgctgccgg ccgtctcgac cgcggcctcg
     6481 aaagcggcac gcgagcccac ggggtcgtcg aagtcccatg tctcgacgac acgcgcttcg
     6541 agctgttgcg gctccatccg gcgtaccacc tctcctggtg cggccaagtc tgcaagccga
     6601 gcctggcgtc ggcaaggcat cgtgcgccgg ggtcctcatg gacctgggaa gggccgcccc
     6661 ggtgcggggc ggcccttccg atgtcgcgac gggtgcggtc ggttcagggc gttgtgctgc
     6721 cgaacacctc cagctcggtg aagtcggtga actgacaacc tgcgaatgca ccgtccgggc
     6781 agttggtggc gaagtccggg acctgtgggc tgttgagcgt cagccgcacg tactgcacgc
     6841 cggccacggc ctggctggcc gcgacgttgg tgtactggaa tcggcccagc cagttggcct
     6901 caccgttgac gtcgacaacc gtggtccacg tcgtcccgtt cggcgacacc tcgatggtga
     6961 agtcaccggt cgacgcgctc cccgcgtcac cgcagccggc ggtcgggtcg accttgaacg
     7021 cggagtctcc tccggaaccg gtaccgatgt cgaccggccg cgccagcttc accacgaggt
     7081 gcttcggcgt catggtgttg gtcggcgtcc cgtcgtcgtc accggtggtg ctgccccaga
     7141 tccggccctg gctcaggtcg atggcacctt cgggtccgca gccgaacggc gtgaggtcgg
     7201 gaccgtcgaa gctgtccacg gcgccgccgg tctgcaggct ggcccagtcc ttgcgcgtct
     7261 cgaagtcgac ccg
//
LOCUS       DATBFZ010000114         4968 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_1707071, whole genome shotgun sequence.
ACCESSION   DATBFZ010000114 DATBFZ010000000
VERSION     DATBFZ010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 4968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4968
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1707071"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="VH419_03500"
     CDS             complement(<1..358)
                     /locus_tag="VH419_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEY7042713.1"
                     /translation="MHPVVKAATGLAAAGLGVLAYSAGYEVRAFRLRETTVPCLPDGV
                     EPLRVLHLSDIHLTPAQERKQEWLESLAALEPDLVVNTGDNLGHVDSVPVLVQSLGAL
                     LDTPGVFVFGSNDYWGP"
     gene            452..910
                     /locus_tag="VH419_03505"
     CDS             452..910
                     /locus_tag="VH419_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020577139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEY7042714.1"
                     /translation="MAELKDRLREQLTASMKARDELRSSTLRMVLTAITNAEVSGKQA
                     RELTDDDVTTVLSSEAKKRREAATAFEDAGRSELAAKERAEAAIIGEFLPEQMTAAEI
                     AELVTATIADLGVAGEGMRAMGRVMGALAPQIKGRADGGAVSAEVRQQLS"
     gene            complement(917..3388)
                     /locus_tag="VH419_03510"
     CDS             complement(917..3388)
                     /locus_tag="VH419_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011753821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEY7042715.1"
                     /translation="MLRRVRPARHEGGLVHQIVVIIGISVLSGALMAGLALPWVGLIS
                     KGAENSASAVKDFPKRLVFKPLNERTRVLASDGSLLATFYDENRKYVTLGKISPIMQQ
                     AIIAIEDARFYEHGALDVRGTLRALFINQAEDKVVQGGSSITQQLVKMTLQENATTAE
                     QRRAAAAKDYARKFEELRYAVWVEDHLSKPEILEHYLNTAYFGDGAYGIEAAAHHYFS
                     TSAATLTLPQAALLAGIVKNPSQYDPTNNPDEARDRRNLVLSKMLDLHIVNTHRARKA
                     IHSPLGLKPSYVSNGCVSTKAPFFCAYLLKYLLADPALGKSTEQRRHLVYGGGLTIQS
                     TIDLRFQHAADKSVRSHVFKTDHAIGALAMVEPGTGYVRAISQSRPMGTNRKAGQTFL
                     NFSVPTKYGDAPGFPAGSTFKLFVLAKAIQMGIPLNKTIFSPPAYPAYLGDYKTCTGH
                     YPSTQTFTFHNSTSSGNMNLYTGTQLSVNTFFVQLEKMTGLCGPWELAREMGEPLDPS
                     RDEPNPDRVPASTLGVSNVHPLEMAEAYATMANRGVHCASTPILEIRDRNGDVIPTPG
                     PQCNRVLKPAYADAVNDILTGVMKPGGFGQDLALDQPSAGKTGTVQPAKSVWFVGYTP
                     TLAAAAMIAGVRPNGDPMSIDYTRIGGVNVGSAHGSTTAGPVWGDAMKAIQKWLPNRS
                     FVPPDPTVVEGQTVSIPSFYGFSPEAAAKELTKLGFNPQIVSYRVNSSAPEGTVAYTS
                     PSYEGTSGETVSIYVSTGYVPPPPSPKPKPKPNPKPPEHTNNPPGPGGNDQPGGNNEP
                     GPGDGGPNGNGNGNGNDHDNRGPGN"
     gene            3646..3957
                     /locus_tag="VH419_03515"
     CDS             3646..3957
                     /locus_tag="VH419_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020577135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HEY7042716.1"
                     /translation="MTWNEHWADVASCKSARPDELFVRGAAQNRAKQVCGQCPVRTEC
                     LAEALDNHIEWGVWGGMTERERRALLRRRPNVTSWRRLLEAAREQHMAVAASSDVTAA
                     G"
     gene            complement(4059..>4968)
                     /locus_tag="VH419_03520"
     CDS             complement(4059..>4968)
                     /locus_tag="VH419_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008356250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HEY7042717.1"
                     /translation="PRAVDGVGAEAGGSLDAMMLDMKRTFDEVVESQATPEKARQILE
                     NPFYVALSSSFAGTQEYMAMEKLGQLHAEALASGRWDLIVVDTPPSRSALDFLDAPER
                     LSSFLDGRLIRILLTPAKGPARLLSSGMFVVTNAIAKVLGGQMLRDVQTFVAAFDTLF
                     GGFRARAEATYQILKDPQTAFVVVASPEADALREAAFFVERLAEENMPLAGLVLNRVT
                     TTGPSSLSTKGAAAAAERLEKESPLTAAVLRLHADRMDRVNRERALRRRFAGAHPSVP
                     TVTVAALATDVHDLDSLREVGDLLLA"
BASE COUNT          769 a         1632 c         1715 g          852 t
ORIGIN      
        1 ccggacccca gtagtcgttg gacccgaaca cgaacacccc gggtgtgtcg agcagcgcac
       61 cgagcgactg cacgaggaca ggcaccgagt caacgtggcc gagattgtcg ccggtgttca
      121 ccacgaggtc gggttcgagc gcggcgaggc tctccagcca ctcctgcttg cgttcctgcg
      181 ccggggtcag gtggatgtcg gacaggtgca gcacgcgaag cggctcgacc ccgtcgggca
      241 gacacggcac cgtcgtctcc cgcagccgga acgcacgcac ctcgtatccc gcgctgtagg
      301 ccagcacacc caacccggct gccgccaggc ccgtcgccgc cttcaccacc ggatgcacct
      361 gtccagcctc ccatcaaccg acttgtcaga agtacatagg gctatagccc tacctgcttc
      421 tgacaagtcg gtgggcccgt agggtcgggg tatggctgag ctgaaggaca ggttgcggga
      481 gcagctgacg gcgtcgatga aggcgcgtga cgagctgcgg tcgtcgacgc tgcggatggt
      541 cctcaccgcg atcacgaacg ccgaggtgtc gggcaagcag gcgcgtgagc tcaccgacga
      601 tgacgtgacc accgtgctgt ccagcgaggc caagaaacgg cgggaggccg caacggcgtt
      661 cgaggacgcc gggcggtccg agctcgccgc caaggagcgt gccgaggcgg ccatcatcgg
      721 tgagttcctg cccgagcaga tgacggcggc ggagatcgcc gaactggtca cggccacgat
      781 cgccgacctc ggcgtggccg gcgagggcat gcgggcgatg gggcgggtga tgggtgcgct
      841 cgccccgcag atcaagggcc gcgccgacgg aggcgccgtc tccgcggagg tccgtcagca
      901 gctcagctag ccgagctcag ttgcccgggc cgcggttgtc gtggtcgttc ccgttgccgt
      961 tgccgttccc gttcggccca ccatcgccgg ggccgggctc gttgttcccg ccgggttggt
     1021 cgttgccacc gggacccgga ggattgttgg tgtgctcggg cggcttcggg ttcggcttcg
     1081 gcttcggctt cggcgacggt ggcggcggca cgtagccggt ggagacatag atgctcaccg
     1141 tctcgccgga ggttccttcg tacgatggtg acgtatacgc gaccgtcccc tccggtgcgc
     1201 tcgagttgac gcggtaggag acgatctgcg ggttgaaccc tagcttggtc agctcctttg
     1261 ctgctgcttc cgggctgaac ccgtagaagg acgggatcga cacggtctgt ccttcgacaa
     1321 cggtcggatc cggcggcacg aagctccggt tcggcagcca cttctggatg gccttcatcg
     1381 cgtctcccca gaccggtccg gccgtcgtcg agccgtgtgc acttccgacg ttgacgccac
     1441 cgatcctggt gtagtcgatg ctcatcgggt cgccgttcgg cctgacgcct gcgatcatcg
     1501 ccgccgccgc gagggtgggc gtgtagccga cgaaccacac cgacttcgcc ggctggacgg
     1561 tgccggtctt gccggccgac ggctggtcga gcgcgaggtc ctggccgaag ccgccgggct
     1621 tcatcacgcc cgtgaggatg tcgttgacag cgtcggcgta cgccggcttg agcacccggt
     1681 tgcactgcgg ccccggagtc gggatcacat cgccgttgcg gtcgcggatc tcgaggatcg
     1741 gcgtcgacgc gcagtgcaca ccccgattgg ccatcgtcgc gtaggcctcc gccatctcga
     1801 gcgggtggac gttcgacact ccaagcgtcg aggcgggaac tcggtctggg ttcggctcgt
     1861 cgcgactggg atcgagcggc tcacccatct ccctggcaag ctcccacgga ccacacagcc
     1921 ccgtcatctt ctccagctgc acgaagaagg tgttcaccga gagctgcgtg ccggtgtaga
     1981 ggttcatgtt cccgctggac gtcgagttgt ggaacgtgaa cgtctgggtc gacgggtagt
     2041 gcccggtgca cgtcttgtag tcgccgaggt acgccgggta cgccggcggg gaaaagatcg
     2101 tcttgttgag cgggatcccc atctggatgg ctttggccag cacgaacagc ttgaacgtcg
     2161 aacccgccgg gaagcccggt gcgtcgccgt acttcgtcgg gacggagaag ttcaggaagg
     2221 tctgtccggc cttgcggttc gtgcccatcg gacgagactg ggagatcgcg cggacgtagc
     2281 ccgtgccggg ctcgaccatc gccagcgcgc cgatcgcgtg gtcggtcttg aacacgtgtg
     2341 agcggacgga cttgtcggct gcgtgctgga agcgcaggtc gatcgtgctc tggatcgtca
     2401 gtccgccgcc gtagacgagg tgtcgacgct gttccgtgga cttgccgagg gccgggtcgg
     2461 cgagcaggta cttgagcagg tatgcgcaga agaacggcgc cttcgtgctg acgcaaccgt
     2521 tgctgacata ggacggcttg agtcccagcg gtgagtggat cgccttgcgt gcgcggtggg
     2581 tgttgacgat gtgcaggtcg agcatcttgc tcaacaccag gttgcgccgg tcacgggcct
     2641 cgtccgggtt gttcgtcggg tcgtactgcg acgggttctt gacgatgccg gcgagcagcg
     2701 ccgcctgcgg cagggtcaac gtcgccgcgc tggtcgagaa gtagtggtgc gccgctgcct
     2761 cgatgccgta ggcgccgtcg ccgaagtacg ccgtgttgag gtagtgctcg aggatctccg
     2821 gcttgctcag gtgatcctcg acccagacgg cgtaccgcag ctcctcgaac ttgcgggcgt
     2881 agtccttggc agcggcggcg cgtcgctgct ctgccgtggt ggcgttctcc tgcagcgtca
     2941 tcttcaccag ctgctgggtg atcgacgacc caccctggac gaccttgtcc tcggcctggt
     3001 tgatgaagag cgcccgaagc gtgccgcgga cgtcgagggc gccgtgctcg tagaaccggg
     3061 cgtcctcgat cgcgatgatc gcctgctgca tgatcggcga gatcttgccg agcgtgacgt
     3121 atttgcgatt ctcgtcatag aacgtcgcca gcagggagcc gtcggatgcc aggacccgag
     3181 tgcgctcgtt gagcggtttg aagacgagcc gcttggggaa gtccttgacg gcgctggcac
     3241 tgttctcggc gcccttgctg atcaacccga cccagggcag ggccaaccca gccatcagcg
     3301 ccccggagag gacgctgatg ccgatgatga ccacgatctg gtggacgagg ccgccttcgt
     3361 gtcgagcagg cctcacgcgc cggagcatgg gtcccagggt acgtgagcgc ctgccggtgc
     3421 cgcatttccg caggtgacca cggcaaagcc cacccgtcac gaggaagacc cgcggcacgt
     3481 cgggaggact agcccggcat agtccgaagt gactagcaag gttgagcagt tcggaccttt
     3541 ccgagttgat cacaccgtcc gtaacgtttc tgtgacctga ggaccacggc cttcgatcgt
     3601 cgcgccgggc tcaggggctc attcttctct cggtaggagc tggacatgac ctggaacgag
     3661 cactgggcgg acgtggcgtc ctgcaaaagc gcccgacccg acgaactctt cgtccgaggt
     3721 gcagcacaaa atcgcgcaaa gcaggtgtgc gggcagtgcc cggtacgcac tgagtgcctc
     3781 gccgaagccc tcgacaacca catcgaatgg ggcgtctggg gcggtatgac ggagcgggag
     3841 cgacgcgcgc tgctccgccg tcgtcccaac gtgacgtcct ggcgccggct gctcgaggcg
     3901 gcgcgggagc agcacatggc ggtcgcggcc tcgtccgatg tgacggctgc cggctgacgc
     3961 gcaccactac aagacgaacc ggtggagacg gctcaggagg gggagtcgtc tcacccggtt
     4021 tgaccaccgg cgtacgacgc tcgggccgcg cgtctcggct acgccagcag caggtcaccg
     4081 acttcgcgga gtgagtcgag gtcgtgcacg tccgtggcca gcgcggcgac ggtgacggtc
     4141 ggcaccgacg ggtgcgcgcc ggcgaaccgg cgacgcagcg cccgctcccg gttgacccga
     4201 tccatccggt ccgcgtgcag gcgcagcacg gccgccgtca acggggactc cttctccagc
     4261 ctttccgcag cggctgcggc acccttggtc gacaggctcg acgggccggt cgtcgtgacc
     4321 cggttgagga cgaggccggc cagcggcatg ttctcctccg ccagccgctc gacgaagaac
     4381 gccgcctctc gcagcgcgtc cgcctcgggc gaggcgacca ccacgaacgc cgtctgcggg
     4441 tccttcagga tctggtacgt cgcctctgcc ctcgcccgga accccccgaa caacgtgtcg
     4501 aacgcggcca cgaacgtctg cacgtcgcgc aacatctggc cgccgagcac cttcgcgatc
     4561 gcattcgtga cgacgaacat cccgctcgac agcagccgcg cgggtccctt cgccggagtc
     4621 agcaggatcc tgatcagccg cccgtcgagg aacgacgaca gccgttccgg tgcgtccagg
     4681 aagtcgagtg ccgaccggga cggcggtgtg tccacgacga tcaggtccca ccgcccgctg
     4741 gccagcgcct ccgcgtggag ctggccgagc ttctccatcg ccatgtactc ctgcgtgccc
     4801 gcgaaggagc tcgagagcgc gacgtagaag gggttctcca ggatctgccg ggccttctcc
     4861 ggggtggcct ggctctcgac cacctcgtcg aaggtgcgct tcatgtcgag catcatcgcg
     4921 tccaggcttc cgcccgcctc cgcgccgacg ccgtcgaccg cccgcggc
//
LOCUS       DATBFZ010000115        15029 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_69944, whole genome shotgun sequence.
ACCESSION   DATBFZ010000115 DATBFZ010000000
VERSION     DATBFZ010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 15029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15029
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69944"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..886
                     /locus_tag="VH419_03525"
     CDS             <1..886
                     /locus_tag="VH419_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007032392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEY7042718.1"
                     /translation="VGKLGGVFRITDGLQKDFGEDRVIDTPLAESGILGTAVGLAMRG
                     YRPVCEIQFDGFVYPAYDQIVSQVAKLHFRSGGSIRMPIVIRIPFGGGIGAVEHHSES
                     PEAYFAHTAGLKVVACSNPVDAYWMIQQAIASDDPVVFLEPKRRYWEKAELDESVAPE
                     PLFASRVVREGSDATLVAYGPMVKTCLEAAAAAEDENRSIEVIDMRTLSPLDMGPVEA
                     SVRKTGRAMVVHEAPVTLGLGAEIAARITEQCFYSLESPVLRVGGFDTPYPPSRIEED
                     YLPDLDRVLDAVDRSLAY"
     gene            908..2404
                     /locus_tag="VH419_03530"
     CDS             908..2404
                     /locus_tag="VH419_03530"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020577848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEY7042719.1"
                     /translation="MSDVKQFKLPDVGEGLTEAEIVTWNVKVGDTVKVNDVIVEIETA
                     KSLVELPCPYAGIVTELLAQEGDTVNVGSPIIAVETGDPGAVTAEAPPEKPEVGSAAE
                     DLVPTPAPDEAVEPGLIGGPAPGGRTSVLVGYGPRTTEAKRRPRKAGATPVETSPATA
                     QSSVQHSFAPASTPAPELSIDEAPLAGVPSESVVARESSQPVGDAAKAKVLAKPPVRK
                     LAKDLGVDLAAVTATGPDGTITRDDVHSHVAAPGQAATVAPIRPTITGERETRVAVKG
                     VRKMTAQAMVSSAFTAPHVTEWVTLDASRTMQMVRRLRDHRDFRDVRVSPLLIVARAV
                     CTAIRRTPEVNAAWDEAAQEIVLKHYVNLGIAAATPRGLMVPNIKDADRLNGVELAKA
                     IGELTTTARDGKTQPAEMTGGSFTITNVGVFGVDSGTPIINPGESAILCMGAIRKQPW
                     VIGNTVKPRWVTTLALSFDHRIVDGEQGSRFLADVAAMVEDPGTALIL"
     gene            complement(2555..4195)
                     /locus_tag="VH419_03535"
     CDS             complement(2555..4195)
                     /locus_tag="VH419_03535"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEY7042720.1"
                     /translation="MTDVTTGRLVTGKWKARLDAKTAEVSSEAKENGSPHVRQDGTAD
                     VIELKPRQAGPTIDAGPTIDAGPTIVAARRIPEIEESRPPAAADRPTRSRPVVHAALR
                     SVRTTMILVDVVTSAVVLGLSTQFYPLAAPVVPVLWLAMMLQRSSRADRMAALTDRRP
                     YLKSSAHLALTLTAIAVLFGSMHSTRVVLGLSALLTVVGLTSRTVVRQMAARGVFNGE
                     ARETVLVVGTRESVCRTIAEWHDLHSIRVVGACLAKADGDVSQVGDVPVLGSVADVAS
                     VARQLGVDIVALHDVDELGGLQLARLQYALEEGGSQLSIITPITNMRVDRGSVRQLGR
                     RVVVDVSYGTPSGLTRRVKTLLDQCLSATLLVLSLPFMLLCAMYIRLTSKGPALFKQT
                     RVGEGGTTFTMYKFRTMRVDAEQQLEALLEKNEVGGGLFKIADDPRVTHAGRLLRRTS
                     LDELPQLLNVLKGEMSLIGPRPALPREVQTYDDLARRRLAVKPGMTGLWQVSGRSNLS
                     WEETVRIDSDYVDNWRPARELSIAMLTIKAVVTRNGAH"
     gene            complement(4726..5922)
                     /locus_tag="VH419_03540"
     CDS             complement(4726..5922)
                     /locus_tag="VH419_03540"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012919424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY7042721.1"
                     /translation="MPGLLDDVVVIDLTRALAGPHAAMMLGDLGARVIKLEVPGSGDD
                     SRGWGPPFVGPVDEPQSTYFMSANRNKESVTVDLKSEEGRTLLRRLVAQGDVVCENFR
                     PGVLERLGFSVAQMHAINPRLIVLSITGFGHDGPEGGRAGYDQIAQGEAGLMSLTGTS
                     SDEPTKVGVPIADLLAGMYGAYGVLAALHERERTGRGRHVRTSLLAAIVGVHAYQGTK
                     WTVAREVPEAIGNAHPSISPYGLFHTADVPVQVACGSESLWRAFAPIVNLDPDDDRFA
                     TNRLRVGRREELTGLIEKTFADHGAEHWLPILAAAGVPAGKVRTVDDVYGWEQTRSQG
                     LLIDVDHPTLGRITLPGPPLRFDELSYAGGRETHLPPPLLGEHNEAVRAWLAVAEVGK
                     LPKQEE"
     regulatory      6014..6082
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            6232..7371
                     /locus_tag="VH419_03545"
     CDS             6232..7371
                     /locus_tag="VH419_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HEY7042722.1"
                     /translation="MFPRSFRPRRSLSLLACVALAVGLVAPASAASAPQAGPVGFRGP
                     ADRAAAAAHWQGGELNRHGVIINSGFQDWGLTIDTIFALAADGRHPVLLHRASKAIRT
                     NYYTHYTRFQGTISANAMAKTLVVAKVIRANAHTFGGHDVRRLVLDRVAGPAAGLEQG
                     RLRDKTTQTYPTDFSSTFGQALGVIGLARSGGVPDDVVSYLLRQRCSDGFFRLQEVPE
                     QTCDEGNASQSAPDVDATALAIEALVSARDHGATLPAGVVAGSVKWLISMQKANGSFG
                     GGVATPGSNTNSTGLAAQALAATGRTKAARAAGVFVRSMQITRAAAGNGPATKDLGAI
                     ALNRQTLNQAIRHGITSTTRGLFLRSTPQAYFALRRVPLGELTAP"
     gene            7368..8474
                     /locus_tag="VH419_03550"
     CDS             7368..8474
                     /locus_tag="VH419_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042723.1"
                     /translation="MTVEATDSGRRVRRAAGVLAAALVVALATTSAGGVGSASADVGT
                     GDPSGRLSGLAGVAAAIATADGTAGYCPDDNGVTVVVDLSALGGDIVVRCAPGPVGSA
                     YDGVDALRDAGFTPTGTQRWGLAFVCRIQGRPAASESLDTDSNSDYHERCVDTPPTDA
                     YWGYWYARNEGEWRYSSAGPKSHQPIEGGFEGWAFSLNHAAGEQPAPGVAPTRPKADP
                     PPTSPSTSPSTPPTSPSPNPEPSRHATSGPAGPRDGSPGSTSSAPTPSTGPSTSAAGP
                     SPTRDRATPQGNPQRTQQQGSRREKTSGEHKAGTPTAAAATTQSGTQVTGELPPVEDG
                     GSAGGVTATVLGAGLLVLLGAGAGIAAWRRARRS"
     gene            8450..9589
                     /locus_tag="VH419_03555"
     CDS             8450..9589
                     /locus_tag="VH419_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008358454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HEY7042724.1"
                     /translation="MATSAALLTARPHRRLPRNLHPVAWWIWALGLAVAASRTTNPLL
                     LGALIGVVTWVVVARRTDAPWAKAFRLYVIAGAVIVVIRVMFRIVFGGDEGEIVLLGL
                     PTVPLPGPSGISLFGDITAESLLAGFYDGLRLATMLICLGGANALANPRRLLRSMPPA
                     LHEVSTAVVVALSVFPQLAESFVRVRRARRLRPEQGRPGGRGVASLVIPVLEDALDRS
                     LLLAASMDSRGYGRSAGRSALMSRVVGTLMVAGLVGLGVGVYGLLDGTAPRILSAPML
                     VAGALVGVCGLWLSGTGVRRTAYRPDSWKLAELVVVASGVTTALCFLVAAHKGLPALD
                     PSLSPLTWPQLAPLPLVGVLVGLIPAWAAPEPLLTISEAMDEVPA"
     gene            9586..11322
                     /locus_tag="VH419_03560"
     CDS             9586..11322
                     /locus_tag="VH419_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY7042725.1"
                     /translation="MIAYDDFSFWYPGKAAAAVSEVSFQVDEGCFALVVGPTGSGKST
                     LLRAVNGLVPHFTGGHVEGRVRTSGHDPRDRPPREYADVVGLVGQDPLAGFVTDTVEE
                     ELAFGLEQLATPPSVMRRRVEEMLDAMGVASLRRRALRTLSGGEQQRVAIGAALVTSP
                     RVLVLDEPTSALDPAAAEEALAAVLRLVHDVGISVVAAEHRLERIVEYADQLVIVDRS
                     RVVDGEPAVMMRSSALAPPVVELGRLLGWDPLALSVRDARRRAGDVRAELERLPADPQ
                     PRSGGGDGPGLEARGIVVRYAGHDAVRGVDLVAEPGEIVALMGRNGSGKSSLLWAIHG
                     AGTLTSGEVRAGGARRRSSRGPVGGRAGGRRARAGRGAAGSPVCLVPQQATDLLFHAS
                     IRAECQAADLSAGLAAGATWALFRDLAGLASGAGQGPRRVAHVDPSGHPRDLSDGQRL
                     ALALAVQLAMDPDVVLLDEPTRGLDYPAKARLAEVLRRLRSEGKVVVVATHDAEFVAG
                     LADRVVLMAEGSVVTDATAAEALTGSMLFAPQVAKVLAPVPLLTVDEVARVIGTMRPA
                     MSAAGEPVVRGG"
     gene            11315..12178
                     /locus_tag="VH419_03565"
     CDS             11315..12178
                     /locus_tag="VH419_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEY7042726.1"
                     /translation="MADGVRNVGGVPAVTTTRPGRGSAAVVVGGRTAGLLVLATLAGL
                     VMFCWPLFVRVPGESFGHVNDAPLLFILVLPLVVAIVLASMAEGGMDSKALAMLGVLA
                     AVNAALRPLGAGLAGIETVFFLLVLAGRVFGPGFGFALGCTSVFASALLTAGVGPWLP
                     FQMMASAWIGLGAGMLPRRVTGRAEVVMLAVYGVLAAYVFGFLMNLWFWPYAVGAGTD
                     VSFVPGDPVVENLRRFVVYTVTTSTLGWDTGRAITNAVAIVVLGPAVLATLRRASRRA
                     SFGSAVSFSDR"
     gene            complement(12456..13775)
                     /gene="paaK"
                     /locus_tag="VH419_03570"
     CDS             complement(12456..13775)
                     /gene="paaK"
                     /locus_tag="VH419_03570"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009073841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047475 - phenylacetate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase PaaK"
                     /protein_id="HEY7042727.1"
                     /translation="MSTLGTLSTPRHLWDDAEQLSIEELRARQLERLRSTLALAYARV
                     PHYKAAFDDAGVRPEDLHELADLARFPSTSKADLRENYPFGMFAVPRKDVVRVHASSG
                     TTGRPTVVGYTADDLDVWSDVMARSIRAAGGRRGDLVHVAYGYGLFTGGLGAHYGAER
                     LGCTVVPVSGGMTERQVQLIVDFRPRIIMVTPSYFLAIVDEMERQGIDPKTSSLEIGI
                     FGAEPWTDEMRREIEERCAMHAVDIYGLSEVMGPGVAQECVETKDGLHIWEDHFYPEI
                     IDPVSGSVVPDGEEGELVFTSLTKQAMPVIRYRTRDLTRLLPGTARPSYRRMEKITGR
                     TDDMMIVRGVNVFPTQIEEQLLRVDGLTPHYVCVLTRPGRMDELTVRVEAHEAGLSDD
                     ARRTLAAQLAGLVKSNVGVSVDVEVTEPHALERSLGKAKRIDDRRSR"
     gene            complement(13772..14236)
                     /gene="paaI"
                     /locus_tag="VH419_03575"
     CDS             complement(13772..14236)
                     /gene="paaI"
                     /locus_tag="VH419_03575"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012924392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyphenylacetyl-CoA thioesterase PaaI"
                     /protein_id="HEY7042728.1"
                     /translation="MTPDELAQACAAAMWADDEASRRLGMTLDRVSSGSATLSMTVRD
                     DMTNGHGIAHGGFIFTLADSAFAFACNSYNERTVAQACDIVFAVPSHLGDRLVAVATE
                     RHRFGRNGIYDIRVTRGTSDGEVIAEFRGRSRSIGGSLVDVGEHGETGGGEG"
     gene            complement(14233..14709)
                     /locus_tag="VH419_03580"
     CDS             complement(14233..14709)
                     /locus_tag="VH419_03580"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY7042729.1"
                     /translation="MRIRPVDDADTESVLALNAESVWALAPLDEERLAWLRSMTRHAL
                     VCEVAGQVAGFALAFEPRTAYDSINYAWHAARFPDFLYLDRVAVSTTYRRRGVATALY
                     DEMESQAASHDRMVCEVNSDPPNEASLAFHRARGYRELGRLVQLDGHEVVLMEKPL"
     gene            14824..>15029
                     /locus_tag="VH419_03585"
     CDS             14824..>15029
                     /locus_tag="VH419_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020389130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaN"
                     /protein_id="HEY7042730.1"
                     /translation="MSPSSDTSTDYFSAHRAMLDDAVEATRTRAYYSAFPESPSPRVY
                     GEKAAEEGAQAFEGWLDGDFSISVP"
BASE COUNT         2220 a         5196 c         5214 g         2399 t
ORIGIN      
        1 cgtcggcaag ctcggcggcg tcttccgcat caccgacggt ctgcagaagg atttcggcga
       61 agaccgcgtc atcgacacgc cgttggcgga gtcgggcatc ctgggcacgg ctgtcggatt
      121 ggcgatgcgt ggctaccgcc cggtgtgcga gatccagttc gacgggttcg tctacccggc
      181 gtacgaccag atcgtcagcc aggtcgccaa gctgcacttc cgcagtggcg ggtcgatccg
      241 gatgccgatc gtcatccgca tcccgttcgg tggcggtatc ggcgcggtcg agcaccacag
      301 cgagagcccg gaggcgtact tcgcccacac ggccggtctc aaggtcgtcg cgtgctccaa
      361 cccggtcgac gcctactgga tgatccagca ggcgatcgct tcggacgacc ccgtcgtgtt
      421 cctcgagccc aagcggcgct actgggagaa ggccgagctc gacgagtccg tcgcgccgga
      481 gccgttgttc gcctcgcggg tcgtccggga gggcagcgac gcgaccctgg tcgcctacgg
      541 tccgatggtg aagacctgtc tggaggccgc ggccgcggcc gaggacgaga accgcagcat
      601 cgaggtcatc gacatgcgca cgctctcacc gctcgacatg gggccggtgg aggcgtcggt
      661 gcgcaagacc ggtcgggcca tggtcgtcca cgaggcgccg gtcacgctcg gcctgggcgc
      721 cgagatcgcg gcgcgcatca ccgagcagtg cttctactcg ctggagtcgc ccgtcctccg
      781 ggttggcggc ttcgacacgc cgtacccgcc gagccggatc gaggaggact acctccccga
      841 cctcgacagg gtgctggacg ctgtcgaccg ttcgctcgcc tactgacacc gaccggatcg
      901 gaacccgatg tctgacgtca aacagttcaa gctccccgac gtgggggagg gtctcaccga
      961 agccgagatc gtcacctgga acgtcaaggt gggcgacacg gtaaaggtca acgacgtgat
     1021 cgtcgagatc gagaccgcga agtcactcgt cgagctgccc tgtccgtacg ccgggatcgt
     1081 cacggagctg cttgcgcaag aaggcgacac cgtcaacgtc ggatcaccga tcatcgccgt
     1141 cgaaacaggc gacccggggg ccgtcacggc cgaagcgcct ccggagaaac cagaggtggg
     1201 cagcgctgcc gaagatctcg tcccgacgcc cgcccccgac gaggcggtcg aacctggtct
     1261 catcggcggt cctgcaccgg gcggtcggac gtcggtgctg gtcggctacg gtccgcgtac
     1321 gacggaggcc aagcgccggc cccgcaaggc cggtgcgaca ccggtcgaga catctcccgc
     1381 cacggcccag tccagcgtcc agcactcgtt cgcgccggcc agcacgccgg ctcccgagct
     1441 gagtatcgac gaggcgccac tcgctggcgt cccgtctgaa tcggtggtgg cccgcgagtc
     1501 gtcccagccg gtcggcgacg cagcgaaggc gaaggtgctg gccaagccgc cggtgcgcaa
     1561 gctggccaag gatctcggtg tcgacctcgc cgccgtgacc gcgacaggtc cggacggcac
     1621 gatcacgcgc gacgacgtac acagccatgt ggctgcccct gggcaggcag ccaccgtcgc
     1681 gccgatccga cctaccatca ccggcgaacg cgagacccgg gtcgctgtca agggcgtgcg
     1741 caagatgacc gcccaggcga tggtcagctc cgcgttcacg gctccgcacg tcaccgagtg
     1801 ggtcacgctc gacgccagcc ggacgatgca gatggtccgc cgactccgcg accaccgcga
     1861 cttccgtgac gtgagggtgt cacccctgct catcgtcgcg cgggcggtgt gcacggcgat
     1921 ccggcgtaca ccggaggtca acgccgcctg ggacgaggcg gcgcaggaga tcgtgctcaa
     1981 gcactacgtg aacctcggga tcgccgctgc gactccgcgc ggtctgatgg tgcccaacat
     2041 caaggacgcc gaccggctga acggggtcga gctcgcgaag gcgatcggcg agctcacgac
     2101 gaccgctcgc gacggcaaga cgcagcctgc ggagatgacg ggcggcagct tcacgatcac
     2161 caacgtcggc gtcttcggcg tcgactcagg tacgccgatc atcaacccgg gcgaatccgc
     2221 gatcctgtgc atgggcgcca tccgcaagca gccatgggtg atcggcaaca ccgtgaagcc
     2281 acgttgggtc accacactgg cgttgtcgtt cgaccaccgg atcgtcgacg gcgaacaggg
     2341 atcgcgcttc ctcgccgatg tcgccgccat ggtcgaggac cccggcaccg cgctgatcct
     2401 ctgacttcac ccgggtctcg gctgttgggc ggtcgagccg gtcggtcagc ggttgctcgg
     2461 ctcgcccgct cgtcggcatc gaggtggcac tcgacgtgcg acacgccgac agaaaccaac
     2521 gaaagctgcc acctcggcgg tggggtgggc ctggtcagtg ggcgccgttg cgggtcacga
     2581 ccgccttgat cgtgagcatc gcgatggaca gttcgcgggc cgggcgccag ttgtcgacgt
     2641 agtcggagtc gattcgcacg gtctcttccc acgacaggtt cgagcggcca ctgacctgcc
     2701 acaagccggt catgccgggt ttgacggcca accgtcgccg ggcgaggtcg tcgtacgtct
     2761 gcacctcccg cggaagcgcc gggcgcgggc cgatcagcga catctcacct ttgagcacgt
     2821 tgagcagctg tgggagctcg tcgagcgacg ttcgccgcag caacctgccg gcatgtgtca
     2881 cgcgcggatc gtcggcaatc ttgaacaggc cgccaccgac ctcgttcttc tccagcagcg
     2941 cctcgagctg ctgctcggcg tccacccgca tcgtgcggaa cttgtacatc gtgaacgtcg
     3001 taccgccctc gccgaccctg gtctgcttga acagcgccgg tcccttcgag gtgagccgga
     3061 tgtacatcgc gcagagcagc atgaacggca gcgacaacac gagcagcgtg gcggacaggc
     3121 attggtccag cagcgtcttg acgcggcggg tgaggccact gggagtgccg tagctcacgt
     3181 cgacgacgac gcggcggccc aactgacgaa ccgaaccccg gtccacgcgc atgttcgtga
     3241 tcggagtgat gatcgacagc tggctgcctc cctcctccag cgcatactgc agccgagcca
     3301 gctgcagacc gccaagttcg tcgacgtcgt gcagtgcgac gatgtcgacg ccgagctggc
     3361 gcgccacgga tgcgacgtca gcgaccgagc cgagcaccgg gacatcgccg acttgcgaca
     3421 cgtccccgtc cgccttcgcc agacacgcgc cgacgaccct gatggagtgc aggtcgtgcc
     3481 actcggcgat ggtgcggcag acgctctccc gcgtcccgac caccagcacc gtctcgcggg
     3541 cctccccgtt gaagaccccc ctcgctgcca tctgcctgac gacggtgcgc gaggtcagac
     3601 cgaccacggt cagcagcgcc gacaacccga ggaccactcg ggtcgagtgc atgctgccga
     3661 acagcacggc gatggcggtg agtgtcagcg cgaggtgcgc gctcgacttg aggtacgggc
     3721 ggcgatcggt caaggcggcc attcgatcgg cccggctcga gcgctgcaac atcatcgcga
     3781 gccacaggac cggtacgacg ggcgccgcca gcgggtagaa ctgcgtcgac agccccagca
     3841 caaccgccga agtgacgacg tcgacgagaa tcatggtcgt ccgcacggac cgcagggccg
     3901 cgtggacgac aggccgcgac cgggtcggcc ggtcggctgc ggctggcgga cggctctcct
     3961 cgatctccgg gatccgccgg gccgccacaa tcgtcggccc ggcgtctatc gtcggcccgg
     4021 cgtctatcgt cggcccggct tgtctgggct tcagctcgat cacgtccgcg gtcccgtcct
     4081 gccggacgtg cggcgaaccg ttctccttcg cctcgctcga cacctcggcc gtcttggcgt
     4141 cgagacgtgc cttccacttt cctgtgacaa gccgccccgt cgtcacgtct gtcatcgcgt
     4201 ctgcttcccc tcgacatccc caccctggat gacggccgga acctcgtcgt cccgcaccgc
     4261 catcacgaca tcaccacggg ttgtgctcat cggggcgaat tcggtcgccc ggatccggca
     4321 cggatcaccc acgcggtggt cgcctcggtg cgcgatcacc ctcgccggat ctggccgcgt
     4381 actcccgatg cgccgctgcc ccgagcgcac gtcccccaac tcccctcagc cccctcagca
     4441 ccgtcctgcc cacgttgccg cggtcgctac cccagttccc gcggccccca cccaagccct
     4501 gctatcggcc gtcctgcttc gtcccgcgaa gcccgatgac cgggattcgc ggcgcacaaa
     4561 ccggcaccga tccggggctc gtggggtcgg cgcgatgcta gtcggggttg tccacccctg
     4621 tacgcggtct cacccaagac ccgggagtcg actacgcagc gtcaccacgc cagtttcggt
     4681 gaaacctttc caccccggga tcagtcacgg actgtcccgt cgtggtcact cctcctgctt
     4741 ggggagtttc ccgacctctg cgacggccag ccacgcgcgg acggcctcat tgtgctcacc
     4801 gagcagaggt ggcggcagat gcgtctcccg ccctccggca tacgacagct cgtcgaagcg
     4861 caacggtgga ccgggcagtg tgatccgacc cagcgtcgga tggtccacgt cgatgagcag
     4921 gccctgcgat cgcgtctgct cccacccgta gacgtcgtcg acggtgcgga ccttcccggc
     4981 gggtaccccg gccgcggcga ggatgggcag ccagtgctcg gcgccgtggt ccgcgaacgt
     5041 cttctcgatc aggccggtga gctcttcacg acggccgacg cgaagccggt tggtggcgaa
     5101 gcgatcatcg tcggggtcca ggttgacgat cggggcgaag gcacgccaca ggctctcgct
     5161 gccgcacgcg acctgcaccg ggacatctgc tgtgtggaag agcccgtacg ggctgatcga
     5221 cgggtgcgcg ttcccgatcg cctccgggac ctccctcgcc acggtccatt tcgtcccctg
     5281 gtaggcatgg acgccgacga tggccgccag cagacttgtc cggacgtgcc gtccgcgtcc
     5341 ggtgcgctcg cgctcgtgca aggctgcgag gacaccgtag gcgccgtaca tgccggcgag
     5401 cagatcggcg atgggcacgc cgaccttggt cggctcgtcc gagctggtac cggtgaggct
     5461 catcagtccc gcctcgccct gggcgatctg gtcgtagcct gcgcgaccac cctcaggtcc
     5521 gtcatgcccg aacccggtga tcgacaacac gatgagccgc gggttgatcg cgtgcatctg
     5581 cgcgaccgag aaaccgagcc gctcgaggac accgggacgg aagttctcgc agacgacgtc
     5641 gccttgtgcg acgaggcgac gcagcagggt ccggccctcc tccgacttca ggtcgaccgt
     5701 cacggactcc ttgttgcggt tggcgctcat gaagtacgtc gactgaggct cgtccaccgg
     5761 accgacaaac ggcggccccc atccacgact gtcgtcgccg gagccaggca cctcgagctt
     5821 gatgacccgg gcgccgagat cgcccagcat catcgcggcg tgtggcccgg ccagcgcgcg
     5881 ggtcaggtcg atgacgacga cgtcgtcgag aaggccaggc atcccgtcac tatgcccgac
     5941 ccaggatcgg ctggtggtac cccctgacgg ctctgctaga ttgcggcgag aactccatac
     6001 agcccagctc caggggaagc cggtgaaagt ccggcactgt cccgcagccg tggatcgaca
     6061 tgacgtcgac agtcggaatg cctggactgg gtgagcggct ccacaactgt cgaggtctgc
     6121 agggtcggga gtccggacat cgtcagcgca gtccaacgcg tgcagcgctc cagcgcacgc
     6181 atgcgtccgc ctggcatccg aacctgggac cgctcgcgaa aggcgtagat gatgtttccc
     6241 aggtccttcc ggccacgtcg ttccctctcc ctccttgctt gcgtcgcgct cgccgtcggg
     6301 ctggtggcac cggcctcggc ggccagtgct ccgcaggccg gtccggtcgg tttccggggc
     6361 ccagccgacc gtgctgccgc tgcggcgcac tggcagggcg gtgagctcaa ccggcacggc
     6421 gtgatcatca acagcgggtt ccaggactgg ggcctcacga tcgacacgat cttcgcgctg
     6481 gcggccgacg ggcgtcaccc ggtgttgctg caccgcgcgt ccaaggcgat ccggacgaac
     6541 tactacacgc actacacgcg cttccagggg accatcagtg cgaacgcgat ggccaagacc
     6601 ctcgtggtgg cgaaggtgat ccgcgcgaac gcgcacacgt tcggtgggca cgacgtacgc
     6661 cgcctggtgc tcgaccgggt ggccggaccc gccgccggct tggagcaggg acggctgcgc
     6721 gacaagacga cccagacgta tccgaccgac ttcagcagca ccttcgggca ggcgctgggc
     6781 gtgatcgggc tggcccgcag cggcggcgtc cccgacgacg tcgtcagtta cctgctccgc
     6841 cagcggtgct ccgacggctt cttccggctg caggaggtcc cggaacagac gtgcgacgaa
     6901 ggcaacgcct cgcagagtgc gccggacgtc gacgccaccg cgctggccat cgaggcactg
     6961 gtctccgcgc gcgaccacgg agccaccttg cccgccggtg tggtcgccgg gtcggtgaag
     7021 tggctgatct cgatgcagaa ggcgaacgga tccttcggcg gcggtgtcgc gacgccgggc
     7081 agcaacacca acagcaccgg gctggcagcg caggcgctcg cagcgacggg caggacgaag
     7141 gccgcccggg ccgctggggt gttcgtccgg tcgatgcaga tcacgagagc ggcggcgggg
     7201 aacgggcccg ccaccaagga cctgggcgcc atcgcactga accggcagac gctgaaccag
     7261 gcgatccggc acggcatcac ctccacgact cgtgggttgt tcctccggtc gacaccgcag
     7321 gcgtacttcg cgctgcgtcg cgtgccgttg ggtgagctga cggcaccgtg accgtcgaag
     7381 ccaccgacag cggtcggagg gtgcggcggg ccgcgggggt gctcgcggcc gcactcgtcg
     7441 tggcgctcgc caccacgtcg gccggcggcg tcggctcggc gtcggcagat gtcggcacgg
     7501 gtgatccgtc gggacggttg tcgggtctcg ccggggtcgc cgcggcgatc gccacggccg
     7561 acggaacggc gggttactgt cccgacgaca acggtgtcac ggtcgtcgtc gacctcagcg
     7621 cgctcggcgg cgacatcgtc gtacgctgcg cccccggacc agtcgggtcg gcgtacgacg
     7681 gggttgacgc cctacgggac gccgggttca caccgaccgg gacacagcgg tgggggctgg
     7741 cgttcgtctg tcggatccag ggccgccccg ctgccagtga gtcgctcgac accgacagca
     7801 actccgacta ccacgaacgt tgcgtggaca cccccccgac cgacgcgtac tggggctact
     7861 ggtacgccag gaacgagggc gagtggagat acagcagtgc tggcccgaag agccatcagc
     7921 cgatcgaagg gggcttcgaa ggctgggcgt tctcactcaa ccatgccgct ggggagcagc
     7981 ccgcgcccgg ggtcgcaccg acccgcccga aggccgaccc gccccccacg tcgccctcga
     8041 cgtcgcccag tacgccgccc acgtcgccga gcccgaatcc cgagccgagc cggcatgcga
     8101 cgagcggtcc ggctggtccg cgggacggca gccccgggtc gacctcgtcc gcacccacac
     8161 cgtcgaccgg accgtcgacc agtgcggccg gaccgtcacc gacccgggat cgcgcgactc
     8221 ctcagggcaa ccctcagcgg actcagcagc agggtagccg ccgcgagaag acgtcggggg
     8281 agcacaaggc gggcacacct accgcggccg ccgctacgac gcagtcaggc acccaggtga
     8341 ccggcgagct cccgcccgtg gaggacggcg gttcggcggg cggcgtgacc gcgaccgtgc
     8401 tcggtgcggg tctgctcgtc ctgctcggag ccggagccgg gatcgcagca tggcgacgag
     8461 cgcggcgctc ctgaccgccc gcccgcaccg ccgactgccg cggaacctgc acccggtggc
     8521 ctggtggatc tgggcacttg gacttgccgt cgcggcgagc cggacgacca acccgctgct
     8581 gctgggcgcc ctgatcgggg tcgtcacctg ggtcgtcgtg gcacggcgga cggacgcacc
     8641 gtgggcgaag gcgttccggc tgtacgtcat cgccggggcg gtgatcgtcg tgatccgggt
     8701 catgttccgg atcgtcttcg gcggcgacga aggcgagatc gtcctgctcg gtctaccgac
     8761 ggtcccgctg cctgggccgt cggggatctc gttgttcggc gacatcaccg cagagtcgct
     8821 gctcgccggc ttctacgacg gccttcggct cgcgaccatg ctcatctgcc tgggtggggc
     8881 caacgcgctg gcgaacccgc gacggctgct ccggtcgatg ccgcctgcct tgcacgaggt
     8941 gagcaccgct gtggtggtcg cgttgtcggt cttcccgcag ttggctgaga gcttcgtgcg
     9001 ggtacgacgc gcgcgccggc tgcgccccga gcagggtcgc cctggtggcc gcggggtggc
     9061 gtcgttggtg atccctgtcc tcgaagacgc gctcgaccgc tcgttgctgc tcgccgcctc
     9121 gatggactcg cgtggctacg ggcgcagcgc tgggcgctcg gcgctgatgt ctcgtgtcgt
     9181 cgggacgctc atggtggcgg ggctcgtggg cctgggtgtc ggcgtctacg ggctgcttga
     9241 cgggaccgcc ccgcgcatcc tctcggcgcc gatgctcgtg gccggagcac tggtcggcgt
     9301 ctgcgggctg tggctgtccg gaaccggtgt gcgtcgaacg gcgtaccggc ccgacagctg
     9361 gaagctggcc gagctggtcg tggtggcgag tggtgtgacc accgcgctgt gcttcctggt
     9421 ggccgcccat aaaggtttgc cggcgctgga tccgagcctg tcaccgctga cgtggccaca
     9481 gctcgcaccg cttccactgg tcggggttct ggtgggtctc atcccggcat gggcggctcc
     9541 ggagccgctg ctcacgatca gtgaggcgat ggacgaggtg ccggcgtgat cgcatacgac
     9601 gacttctcgt tctggtaccc gggcaaggcg gcggctgcgg tgtccgaggt ctcgttccag
     9661 gtggacgagg gctgctttgc gctggtcgtc gggccgaccg ggtcgggcaa gtcgacgctg
     9721 ttgcgggcgg tgaacgggct tgtgccgcac ttcacgggtg gccacgtgga aggccgagta
     9781 cggacctccg gccacgaccc gcgagaccgt ccgccgcgag agtacgccga cgtggtcggt
     9841 ctggtgggcc aggatccgtt ggcgggtttc gtcaccgaca cggtcgagga ggagctggcc
     9901 ttcggcctcg aacagctggc cactccgccg tcagtgatgc ggcgacgggt ggaggagatg
     9961 ctggacgcga tgggggtcgc gtcgctgcgg cggcgcgcgc tccggaccct ctccggtggg
    10021 gagcagcaac gggtggccat cggtgccgcc ctggtgacgt cgcctcgggt gctggttttg
    10081 gacgagccga cgtcggcgct ggaccctgcc gccgccgagg aagcgctcgc cgccgtgctc
    10141 cgactggtgc acgacgtggg catcagcgtg gtcgcggctg agcaccggct ggagcggatc
    10201 gtcgagtatg ccgaccagct cgtgatcgtc gacaggtcgc gggtcgtcga cggagaaccc
    10261 gctgtcatga tgcggtcgtc cgcgttggcg ccaccggtgg tcgagctggg acggttgctc
    10321 ggctgggacc cgctggcgct gtcggtccgg gacgcgcggc gacgagccgg cgacgtacgg
    10381 gccgagctgg agaggctgcc cgcggacccg cagcctcgct cgggcggtgg cgacgggccg
    10441 ggactcgagg cgcgggggat cgtcgtccgg tacgccggtc acgacgcagt ccgcggcgtc
    10501 gacctggtgg ccgagcccgg ggagatcgtg gcgctgatgg ggaggaacgg gtcgggcaag
    10561 tcctcgctgc tctgggcgat ccacggagcc gggacgctga cgtcgggtga ggtgcgtgcc
    10621 ggtggcgctc gccgccgttc gtcgcgtggt ccggtgggcg gtcgcgccgg cggtcgtcgg
    10681 gcgcgtgctg gtcgcggggc cgccgggtcg ccggtctgtc tggtaccgca acaggcgacg
    10741 gacctgctgt tccatgcgtc gatccgggcc gagtgccagg cggccgacct gtccgcaggt
    10801 cttgcggcgg gtgccacctg ggcgttgttc cgcgacctgg ccggtctcgc ctcgggcgcg
    10861 ggccagggtc cgcgccgcgt cgcgcatgta gaccccagcg gtcatccgcg agatctctcc
    10921 gatggtcagc ggctcgcgct ggcgctggcg gtgcagctgg cgatggaccc cgacgtcgta
    10981 ctcctcgacg agccgacgcg cggtctcgac tatccggcga aggcgcggct cgcggaagtc
    11041 ctgcgccggc tccgttcgga aggcaaggtc gtcgtggtgg cgacccacga cgcggagttc
    11101 gtcgcgggac tcgccgaccg ggtcgtcttg atggcggaag gttcggtggt cacggatgcg
    11161 accgcggccg aggcgctgac cggctcgatg ctgttcgcgc cacaggtggc gaaggtgctg
    11221 gccccggtgc cgctgctgac ggtcgacgag gtggcgcggg tgatcgggac catgcggccg
    11281 gccatgtctg ccgcaggtga gccggtggtg cgcggtggct gacggcgtgc gcaacgtggg
    11341 cggcgtgccc gccgtgacga cgacccggcc tggtcgtggg agcgcggcgg tggttgtggg
    11401 tggccggacc gccgggttgc tggtcctggc cacgctggcc gggctggtga tgttctgctg
    11461 gccgctgttc gtgcgtgtcc cgggcgagtc gttcggtcat gtcaacgacg caccgttgct
    11521 gttcatcctc gtgctgccgc tcgtggtggc catcgtgctc gcatcgatgg ccgagggcgg
    11581 gatggactcg aaggcgctgg ccatgctcgg cgtcttggcc gccgtcaatg ccgcactgcg
    11641 tcccctcggt gcagggttgg cagggatcga gacggtgttc ttcctgctgg tgctcgccgg
    11701 gcgggtgttc gggcccggct tcgggttcgc cttgggctgt acgtcggtgt tcgcgtccgc
    11761 gctgttgacg gctggagtcg gtccgtggct gccgttccag atgatggcct cggcgtggat
    11821 cgggctcggc gccgggatgc tgccgcgtcg ggtgacggga cgcgctgaag tcgtgatgct
    11881 ggccgtctac ggcgtgctgg cggcttacgt cttcgggttc ttgatgaacc tgtggttctg
    11941 gccgtatgcc gtcggcgccg ggaccgacgt gtcgttcgtg cccggcgacc cggtcgtgga
    12001 gaacctccgg cgcttcgtcg tctacaccgt gacgacgtcg accctcggct gggacaccgg
    12061 gcgggccatc acgaacgcgg tggccatcgt cgtactcggc ccggccgtgc tcgcgacgct
    12121 acgccgcgcc tcccgccgcg cctccttcgg ctccgccgtc tccttcagtg atcgctgagg
    12181 gtttgcgatg tttacagccc aagcggcgtg tagacatcgc aaaccttcga tgggcacagt
    12241 gggtctcgga tgtggacatc gcaaacccca gactggtcag ctgcggatgt ccgaaaaccg
    12301 ccagtcaaga agggccgaat gccagcaagc cagctcagcg ggcagcgaca ccaccgctcc
    12361 tatctgccga tgcaccccgg ccggcgccga ccgaacgccg ggcgcccacg ccctccactg
    12421 gcgaccacct ccgcgcacag aatgttagga ggaggtcagc gggagcggcg gtcatcgatg
    12481 cgcttcgcct tgcccagtga gcgttcgagg gcgtgcggct cggtcacctc cacgtccacg
    12541 ctcacgccga cgttggactt cacgagcccg gccagctgtg ccgccaacgt acgacgtgca
    12601 tcgtcggaca ggcccgcctc gtgcgcctcg acccggaccg tcagctcgtc catccgacct
    12661 ggccgggtca gcacgcagac gtagtgcgga gtcaacccgt cgaccctgag caactgctcc
    12721 tcgatctgcg tcgggaacac gttgacccct cggacgatca tcatgtcgtc ggtgcgtccg
    12781 gtgatcttct ccatccggcg gtacgacggc cgggctgtcc ccggaagcag tcgtgtcagg
    12841 tcacgggtcc ggtagcggat caccggcatc gcctgtttcg tcaacgacgt gaagacgagc
    12901 tcaccctcct caccgtcggg tacgacggaa ccggagaccg ggtcgatgat ctccggatag
    12961 aagtggtcct cccagatgtg caggccgtcc ttcgtctcca cacactcctg cgcgacacct
    13021 ggtcccatca cctcactgag gccatagatg tcgaccgcgt gcatcgcgca ccgctcctcg
    13081 atctcgcggc gcatctcgtc cgtccacggc tcggcgccga agatcccgat ctccagcgac
    13141 gacgtcttgg ggtcgatccc ctggcgctcc atctcgtcga cgatggcgag gaagtacgac
    13201 ggcgtcacca tgatgatgcg cggtcggaag tcaacgatga gctgcacctg tcgttcggtc
    13261 atgccgccgc tcaccgggac caccgtgcag ccgagccgct cggcaccgta gtgggccccg
    13321 aggccgccgg tgaacaggcc gtagccgtag gccacgtgga ccaggtcccc gcgccggccg
    13381 ccggcggccc tgatcgagcg cgccatcacg tccgaccaga cgtccaggtc gtcagcggtg
    13441 tagccgacaa cggtgggccg gccggtcgta ccgctcgagg catggacgcg tacgacgtcc
    13501 ttgcggggta cggcgaacat gccgaacggg tagttctcgc gcaggtccgc cttcgacgtg
    13561 gacgggaacc gggctaggtc tgcaagctcg tggaggtctt ctggtcgtac tcccgcgtcg
    13621 tcgaacgccg ccttgtagtg cggaactcgc gcgtaggcga gggcgagtgt ggaccgcagc
    13681 cgttcgagct gacgggcgcg cagctcctcg atcgacagct gctccgcatc gtcccagagg
    13741 tggcgtggtg tcgacagcgt ccccaacgtg ctcatccttc cccgccacct gtctcgccgt
    13801 gctcccccac gtccaccaga ctgccgccga tcgaccggct gcgaccacgg aactccgcga
    13861 tcacctcacc gtccgacgtt ccgcgggtca cccggatgtc gtagatgccg ttgcgcccga
    13921 accgatgccg ctcggtcgcc accgccacga gccggtcgcc gagatgagac gggacggcga
    13981 acacgatgtc gcacgcctgg gcgacagtcc gctcgttgta cgagttgcag gcgaacgcga
    14041 acgccgagtc ggcgagggtg aagatgaacc cgccatgcgc gatgccatga ccgttcgtca
    14101 tgtcgtcgcg gaccgtcatc gacaaggtgg ccgatccgct gctcacgcga tcgagcgtca
    14161 taccgagtcg gcgcgatgcc tcgtcgtccg cccacatcgc ggctgcacac gcctgcgcga
    14221 gctcgtccgg agtcacaacg gcttctccat caacacgacc tcgtgcccgt cgagctggac
    14281 gaggcgtccg agctcgcgat aaccgcgtgc tcggtggaac gcgagcgacg cctcgttggg
    14341 cgggtccgag ttcacctcgc acaccatgcg gtcgtggctc gctgcctgag actccatctc
    14401 gtcgtacagc gcggtcgcga ctccgcgccg ccggtacgtc gtagacacgg cgacccggtc
    14461 gaggtacagg aagtcgggga agcgtgctgc gtgccacgcg tagttgatcg agtcgtacgc
    14521 cgtccgtggc tcgaacgcca acgcgaaacc ggccacttgg ccggccacct cgcacaccag
    14581 tgcatgcctc gtcatggacc gcagccaggc gagacgttcc tcgtcgagcg gtgcaagcgc
    14641 ccagaccgac tcggcgttca aggccagaac cgactcggtg tctgcgtcgt cgacggggcg
    14701 gatcctcacc ctcggacctt ctcacgcgtc cagccggcag cgcacccggc cgggtcacga
    14761 cgtagggtcg aagtcgtcgg ggccgaaacc cggcgagaac gccagcgacc cagcgaggat
    14821 cccgtgagcc cgtcatccga cacctccacc gactacttca gcgcccaccg ggccatgctc
    14881 gacgacgccg tcgaagcgac gaggaccagg gcgtactact cggccttccc ggagtcgccg
    14941 agcccgcggg tgtacggcga gaaggctgcc gaggaaggcg ctcaggcgtt cgaggggtgg
    15001 ctcgatggcg acttctccat ttctgtgcc
//
LOCUS       DATBFZ010000116         2871 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_3023059, whole genome shotgun sequence.
ACCESSION   DATBFZ010000116 DATBFZ010000000
VERSION     DATBFZ010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2871
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3023059"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1886)
                     /locus_tag="VH419_03590"
     CDS             complement(<1..1886)
                     /locus_tag="VH419_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase central domain-containing
                     protein"
                     /protein_id="HEY7042731.1"
                     /translation="MRATPPPQGLYDGLHEHDACGVAFVATLTGVPSHDIVDKALTAL
                     RNLEHRGASGAEPDSGDGAGILIQVPDAFLRSECGFDLPPAGGYAVGTAFIPGDAEAI
                     ATTKQHIAELAEDEGLRVLGWRDVPTAPDMLGATARGCMPSFAQLFVAPATRRVVGMA
                     LERMAYCLRKRAEHESAAYFPSLSSRTLVYKGMLTTEQLDDFFPDIQDERLASAMAVV
                     HSRFSTNTFPSWPLAHPYRFIAHNGEINTVKGNRNWMKARESLLRSDLFAKASTAAPS
                     DTGLERIFPIVTPEASDSASFDEALELLHLGGRSLPHAVLMMIPEAWENHAEMDPQRR
                     AFYEFHSTLMEPWDGPACVVFTDGTQIGAVLDRNGLRPSRFWVTDDGLVVLASEVGVL
                     DLPPASVVRKGRLQPGRMFLVDVDEQRIIEDDEIKQALAAEAPYEEWLHAGLMKLEDL
                     PARQHIVHTHASVTRRQQVFGYTEEELRVLLTPMARNGVEPIGSMGTDSPIAALSERP
                     RLLFDYFSQLFAQVTNPPLDAIREELVTSLAGTIGPEGNLLDPGPASCRQVVLGFPVI
                     DNDELAKLIHINRDGDLPGFDAHVVKGLYDVHHGGDALAERLDAICAEVSEAIAEGAR
                     IIVLS"
     gene            complement(2067..2348)
                     /locus_tag="VH419_03595"
     CDS             complement(2067..2348)
                     /locus_tag="VH419_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013161016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEY7042732.1"
                     /translation="MARFDEVIHPSTRLSIVSLLAAVDWVDFAFVRDRLNLSDSALSK
                     QFATLEEAGYLTIDRRVVDGRRRVRVCLTRSGRKAFDGHVAALRQIVAI"
     gene            complement(2351..2833)
                     /locus_tag="VH419_03600"
     CDS             complement(2351..2833)
                     /locus_tag="VH419_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042733.1"
                     /translation="MARDITDSEARDALDTIERSRHAVIAEIGIPRWYWWGLAAGWVC
                     LGLAADFANAWVGLVLTFAFGAANAVVAQRAVSGRRRTGQLSVRADVVGRHLPALVLA
                     AVAGLGLVTSVAGWLLWADGAQHPATIASVIVAVVILLGGPHVNAWARRRAARSRSFQ
                     "
BASE COUNT          489 a          967 c          974 g          441 t
ORIGIN      
        1 gacagcacga tgatgcgcgc gccctcggcg atcgcctccg acacctcggc gcagatcgcg
       61 tcgagccgct ccgccagcgc gtcgccgccg tggtgcacgt cgtacaggcc cttgacgacg
      121 tgggcgtcga agccgggcag gtcaccgtcg cggttgatgt ggatgagctt ggcgagctcg
      181 tcgttgtcga tcaccgggaa gccgagtacc acctggcgac acgacgcggg cccagggtcg
      241 agcaggttcc cctctggccc gatcgtgccc gccaacgacg tgacgagctc ctcgcggatg
      301 gcgtccaggg gcgggttcgt cacctgcgcg aacagctggc tgaagtagtc gaacagcagc
      361 cgcggtcgct cggagagggc ggcgatcggg ctgtcggttc ccatcgagcc gatcggctcg
      421 acaccgttgc gggccatcgg cgtcaggagg acgcgcagct cctcctcggt gtagccgaag
      481 acctgttgcc ggcgcgtgac cgacgcgtgc gtgtgcacga tgtgctggcg ggctggcagg
      541 tcctcgagct tcatcaggcc cgcatgcagc cattcctcgt acggcgcctc cgcggcgagg
      601 gcttgcttga tctcgtcgtc ctcgatgatg cgctgctcgt cgacatccac caggaacatc
      661 cgacccggct ggagccgccc cttgcgaacg acgctcgcag gtggcaggtc gaggacacca
      721 acttccgacg cgaggacgac aagtccgtcg tcggtcaccc agaaccggga cggacgcagc
      781 ccgttgcggt ccagcacagc cccgatctgc gtcccgtcgg tgaagaccac acacgcgggg
      841 ccgtcccagg gctccatcag ggtcgagtgg aactcgtaga aggcccgacg ctgcggatcc
      901 atctccgcgt ggttctccca cgcctccggg atcatcatca gcaccgcatg tgggagcgac
      961 cgaccgccga ggtgcagcag ctccagcgcc tcgtcgaacg acgcggagtc ggacgcctcg
     1021 ggtgtgacga tcgggaagat ccgctccagg ccggtgtccg acggcgcagc tgtactggcc
     1081 ttggcgaaca ggtccgatcg cagcagcgac tcgcgcgcct tcatccagtt gcgattgccc
     1141 ttgaccgtgt tgatctcgcc gttgtgcgcg atgaagcgat acggatgggc cagcggccac
     1201 gacgggaacg tgttggtcga gaaccgtgag tgaacgaccg ccatcgcaga cgccaaccgc
     1261 tcgtcctgga tgtcggggaa gaagtcgtcg agctgttcgg tcgtcagcat gcccttgtag
     1321 acgagcgtgc gagacgacag ggatgggaag taggccgcag actcgtgctc ggcccgcttc
     1381 cggaggcagt aggccatccg ttccagcgcc atgccgacca cacgcctggt cgccggtgcc
     1441 acgaacagct gcgcgaacga cggcatgcac ccacgagcgg tggcgccgag catgtccggt
     1501 gccgtcggta cgtcgcgcca accgagaaca cgcagtcctt cgtcttcggc cagctcggcg
     1561 atgtgctgct tcgtcgtcgc gatcgcttcg gcgtcacccg ggatgaacgc cgtaccgacg
     1621 gcatagccac cggcaggcgg aagatcgaaa ccgcactccg aacgcaggaa cgcgtcggga
     1681 acctggatca ggatcccggc gccgtcaccc gagtcgggtt cggcaccgga cgcaccacga
     1741 tgctcgaggt tgcgcagcgc cgtgagcgcc ttgtcgacga tgtcgtgtga gggcacgccg
     1801 gtcaacgtgg cgacgaaggc gaccccacag gcgtcgtgct cgtgcagccc gtcgtacagc
     1861 ccctggggcg gcggggtggc tcgcatggca ggactcccgt cgtcctgcga caacgtcacc
     1921 tggagggtgg ccggtcgcga tgctgatctc gtgcgctgat gagagggcgc cgggacgacg
     1981 ttggccctgc gatgttcggc gagactacag cagcgtaaca acgctggtca ccgccgtggc
     2041 ccaccattcg gacgtgtcgg agtcggtcag atggcgacga tctggcgcag cgccgcgacg
     2101 tgaccgtcga aagccttccg cccggacctg gtgaggcaga cccggacgcg acggcgtccg
     2161 tcgaccaccc gtcggtcgat ggtgaggtag ccggcttctt ccagggtggc gaactgtttc
     2221 gacagtgccg agtcggacaa gttcagccgg tcgcgcacga acgcgaagtc gacccagtcg
     2281 accgcggcga gcagcgacac gatcgacagc cgggtgctcg ggtggatgac ctcgtcgaac
     2341 cgagccatgg tcactggaag gaccgcgatc gagcagcacg gcggcgcgcc cacgcattga
     2401 cgtgtggccc gccgagcagg atcaccaccg cgacgatcac gctggcgatc gtggccgggt
     2461 gttgcgcacc gtcggcccac agcagccagc ccgccacgga ggtgaccagt ccgagccctg
     2521 cgacggccgc gagcaccagg gcaggaaggt gtcggccaac gacgtcggct cgcacgctga
     2581 gctgcccggt acgacgccgc ccgctgaccg cccgctgggc aacgaccgcg ttcgccgcgc
     2641 cgaacgcaaa cgtcagaacg aggccgaccc aggcattggc gaagtcggct gccagcccga
     2701 ggcacaccca gccggccgcg aggccccacc agtaccaccg tggaatgccg atctcggcga
     2761 tgactgcatg acggctgcgc tcgatcgtgt cgagggcatc ccttgcctcg ctgtcggtga
     2821 tgtcacgtgc catgatttcc ccctactttc ctacttggaa agtcaacgtt a
//
LOCUS       DATBFZ010000117         2181 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_2366861, whole genome shotgun sequence.
ACCESSION   DATBFZ010000117 DATBFZ010000000
VERSION     DATBFZ010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2366861"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(11..862)
                     /locus_tag="VH419_03605"
     CDS             complement(11..862)
                     /locus_tag="VH419_03605"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEY7042734.1"
                     /translation="MGANDRQPSTGSGTARSAIDVKTLLVTLAVAATLLLAYAVGSSQ
                     RSPSTSAATEGAAAADTSSGSAATDADQQQATIVMTGTGKAIGVPDQMTFQVEVEATS
                     SDVSSALAQVSRTANKVLHRLRDNDVPRKNVKTNGLSIHPDYDYSGGRAVIVGYSASE
                     HMVVAIDDLSAAGRVLGATADAGGNAVRINGIRLSISDTDALLQQARRDAVKDATTKA
                     DEYAAAAGRALGEVVSIREVTQGSSIRPVPQALEKLSAADGTVVPIRPGRSALQVTVA
                     VVWTFAD"
     gene            complement(927..>2181)
                     /locus_tag="VH419_03610"
     CDS             complement(927..>2181)
                     /locus_tag="VH419_03610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEY7042735.1"
                     /translation="YGSRVLVVAGGGDNGGDALFAGASLARRGVRVEALLVGSHAHEA
                     GLAALRRCGGYVVQAPDDRYDLVVDGVVGIGGRPGLPDEIAEVFDRVEGLVVAVDVPS
                     GVEVDTGQLDGRHVRADVTVTFGTHKVAHLVAPAAYACGVVEYVDIGLGDLLPGPAVE
                     VLQQDDVRRLLPQPVPSSHKYTRGVLGVVAGSPQYTGAGVLVVAAANRTGLTGMIRYE
                     GASEALVRAAHPEVVIGPGQVQAWVVGSGIGRGLASQVSSVLSERLPTVVDADGLRHL
                     PKTCQAPTVLTPHAGELANMLDLPRSQVEAEMLASSQEAAERWNAVVLLKGGRSLIAA
                     PDGRVRANTTGVPWLATAGAGDVLSGVVGALLASGLDAFDAASAGAWLHGSAATLASA
                     GGPISASDVVAALPETILRTLVSTS"
BASE COUNT          326 a          784 c          748 g          323 t
ORIGIN      
        1 ccgcaccagg tcagtcggcg aacgtccaga ccacggcaac ggtgacctgc agtgccgacc
       61 tgccagggcg gatcggcacg accgttccgt cggctgcact cagcttttcg agcgcctgtg
      121 gcacgggacg gatggagctc ccctgtgtca cctcgcgaat cgacacgacc tcaccgagtg
      181 cgcgaccagc tgcggcggcg tactcgtcgg ccttcgtcgt ggcgtccttc acggcgtcgc
      241 ggcgcgcctg ctggagcagc gcgtcggtgt cggagatgct gagccggatc ccgttgatgc
      301 gcaccgcgtt cccgccggcg tcggctgtgg cacccaggac acgtcctgcg gcggacaggt
      361 cgtcgatggc aacgaccatg tgctcggacg ccgagtaccc gacgatgacc gcgcggccac
      421 cggagtagtc gtagtcggga tggatggaca gtccgttggt cttgacgttc ttccgcggta
      481 cgtcgttgtc gcgaaggcgg tgcaggacct tgttcgccgt cctgctcacc tgtgccagcg
      541 ccgaggacac gtccgacgag gtggcctcga cctcgacctg gaatgtcatc tggtcgggta
      601 cgccgatggc cttgcccgtc cccgtcatca cgatcgtcgc ctgttgctgg tcggcgtctg
      661 tggcggccga cccggacgag gtgtccgccg cggcagcacc ttcggttgca gcgctggtgc
      721 tggggctgcg ctggctcgag ccgaccgcgt aggcgagcag caaggtcgct gccaccgcca
      781 gcgtcacgag gagggtcttg acgtcgatcg ccgacctcgc cgtacccgag cctgttgagg
      841 gttgtcgatc gttcgcgccc atgcagcatc actcctcgat atcggtcgtt cgaggatttg
      901 acgcgggcgt cgcggcagca gttccactag gacgtggaca caagcgtccg caggatcgtc
      961 tcgggcagtg cggccacgac gtcgctcgca ctgatcggcc ccccggccga ggccagggtc
     1021 gcggcggagc cgtgcaacca ggcaccggcc gaagcggcgt cgaaggcgtc gagccccgat
     1081 gcgagcaggg caccgaccac gccgctcagc acgtcgccgg caccagcggt cgccagccac
     1141 gggactccgg tcgtgttcgc tcggacccgc ccgtctggag cggcgatcag cgatcggccg
     1201 cctttgagca gcacgacggc gttccagcgt tcggctgctt cctggctgga cgccaacatc
     1261 tcggcctcga cctgggagcg gggaaggtcc agcatgttcg cgagctcccc cgcgtgcggc
     1321 gtgagcacgg tcggcgcctg gcaggtcttg ggcaggtgac gcagcccgtc ggcgtcgacc
     1381 acggtcggca gtcgctcgga gagcaccgag gacacctggg acgcgagccc gcggccgatg
     1441 cccgacccca cgacccacgc ctgcacctgg ccagggccga tgaccacctc ggggtgggcg
     1501 gcccgcacca gtgcctcgct ggcgccctcg taacggatca tccccgtcag gccggtccgg
     1561 ttggccgctg cgacgacgag gacacccgcg ccggtgtact gcggcgaccc cgccacgacc
     1621 ccgagcacgc cgcgcgtgta cttgtgcgag ctcggcacgg gctgcgggag cagccggcgt
     1681 acgtcgtctt gctggagcac ctcgacggcc gggccgggga gcaggtcgcc gagcccgatg
     1741 tcgacgtact cgacgacacc acaggcgtac gccgcggggg cgaccaggtg tgccaccttg
     1801 tgcgtcccga acgtcaccgt gacgtcggcc cggacgtggc gcccgtccag ctgcccggtg
     1861 tccacttcga cgccggaggg gacgtcgaca gcgaccacga gaccctcgac ccgatcgaac
     1921 acctccgcaa tctcgtcagg caggcccggc cgtccgccga taccgaccac gccgtcgacg
     1981 accaggtcgt accgatcgtc gggagcctgg acgacgtacc caccgcaacg acgcagcgcg
     2041 gcgagccccg cctcgtgtgc gtgtgagcca acgagcaggg cttcgacgcg cacaccgcgt
     2101 ctggccagcg aggcgcccgc gaacaacgcg tcaccaccgt tgtcaccgcc accggcgacc
     2161 accaggaccc gtgaaccgta g
//
LOCUS       DATBFZ010000118         2966 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_5944165, whole genome shotgun sequence.
ACCESSION   DATBFZ010000118 DATBFZ010000000
VERSION     DATBFZ010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2966
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5944165"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="VH419_03615"
     CDS             <1..184
                     /locus_tag="VH419_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017936274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042736.1"
                     /translation="PNWTLHAAIEQDYAGPGVRWPDPWLPGRRKPQTGRTDGPKPRLQ
                     LIREGEPRVHHGRWRP"
     gene            328..732
                     /locus_tag="VH419_03620"
     CDS             328..732
                     /locus_tag="VH419_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020577551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HEY7042737.1"
                     /translation="MQSETRSYRTGDRDVVLDLTSDCAAFVRRQGDGLLHVFVPHATA
                     GLAILETGSGSDDDLLASLDDLLPADDRWRHRHGSRGHGRSHVMPALVPPYATIPVID
                     GRLGLGTWQRICLVDINVDNAEREVRLSFLTG"
     gene            complement(893..2020)
                     /gene="gcvT"
                     /locus_tag="VH419_03625"
     CDS             complement(893..2020)
                     /gene="gcvT"
                     /locus_tag="VH419_03625"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEY7042738.1"
                     /translation="MGEDTTDNLLTSVLHERHVALGAKFAEFSGWSMPLEYSGAVKEH
                     NAVRSAVGVFDVSHLGKAVVHGPGAAAYVNSCLTNDLGKISPGKAQYTLCCVDDGGVV
                     DDLIAYLRGDDEVFLIPNAANTTEVVRRLADDAPEGIVVENLHRDYAILAVQGQRSDD
                     LLADLGFPTGHEYMSFVELEHDGAPVVVCRTGYTGERGYELVVPNAVAVSVWDALMAH
                     GAEHGLVPAGLGARDTLRTEMGYPLHGQDISRTITPVQARLGWAVGWKKDAFWGADAL
                     RAEREAGPRRLLRGLVAAGRGIPRPHMQVLAPDSTGQADEAPQVIGEVTSGTFSPTRK
                     VGVALALLDPGVSDGDEVLVDVRGRHEAFLVTKPPFVTPST"
     gene            2135..>2966
                     /locus_tag="VH419_03630"
     CDS             2135..>2966
                     /locus_tag="VH419_03630"
                     /inference="COORDINATES: protein motif:HMM:NF014808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family peptidase N-terminal
                     domain-containing protein"
                     /protein_id="HEY7042739.1"
                     /translation="MITKSASTSATLRNTSARSIKADAVVIGIHGSAGNGSGIAAAAG
                     AADVADGFGKRFATMLEDLGASTKVGDTCKLPSADSAKAPLVVTVGLGPEPDDELDLE
                     ALRRAAGAAARAAAGKKTVVLALPASTDDQIRAVVEGALLGNYGYDRYLTKKHEPVAE
                     FVVVSEVARSRTARKALADATAVAAAVNFCRDLVNAPPNDLYPESFAKQVKDASKGTD
                     VTVSITDEKALESKGYGGIMGVGRGSVRPPRLVKLSYRPAKPVAHLAFVGKGITFDSG
                     G"
BASE COUNT          493 a         1031 c          995 g          447 t
ORIGIN      
        1 cccgaactgg acgttgcacg ccgcgatcga gcaggactac gccggtccgg gtgtgcgctg
       61 gccggacccg tggctaccgg ggcggcgcaa accgcagacc ggccggaccg acggccccaa
      121 accgcgcctg cagctcatcc gtgaaggaga acctcgcgtc caccacggtc gctggcgccc
      181 ctgagggtgg gaggtttgcg atgtttacag ccctttcggg ctgtaaacat cgcaaaccct
      241 cgatggaagc agcgagtctg ggatgcagac ccgttcactc ggggacgcgg actgatcgcc
      301 gccagctgcg gatgcgacca tgacacgatg cagagcgaga ctcgcagcta tcgcaccggc
      361 gaccgtgacg tcgtactcga tctgacgagt gactgcgcgg cgttcgtacg ccggcaaggc
      421 gacgggctgc tccacgtctt cgtgccgcac gccacggcag ggctggccat cctcgagacc
      481 ggttcaggca gtgacgacga cctgctcgcg tccctggatg acctgttgcc ggccgacgac
      541 aggtggcgac accgtcacgg gagccgcggt catggccggt cgcacgtgat gccggcgttg
      601 gtcccgccgt acgcgacgat tcccgtcatc gacggtcggc tcgggctcgg cacctggcag
      661 cgcatctgtc tcgtcgacat caacgtcgac aacgccgagc gcgaggtgcg gctttcgttc
      721 ttgaccgggt gaactatccg gccggtccac tggtcgcgcc cgtcgtggtt tgcctgggta
      781 gccatccaaa cggacgggta gccaggcagg tctgcctggg gagggatggg tttggctggt
      841 tagccatgca aaccgccgcc cgtgaccgac ggatgtggct gcgcacccgg cgctaggtgg
      901 agggggtgac gaacggaggt ttggtgacga ggaaggcctc gtgccgacct cgtacgtcga
      961 caagcacctc gtctccgtcg gaaacgccgg gatcgagcaa cgccagcgcc acgcccacct
     1021 tccgggtcgg cgagaacgtc cctgacgtca cctctccgat gacctgcgga gcctcgtcgg
     1081 cctgcccggt cgagtcgggg gccaacacct gcatgtgggg gcgtgggatt ccacgccccg
     1141 ctgcgaccag ccctcgcagc agtcgccgtg gcccagcctc gcgctccgcg cgcaacgcat
     1201 cggctcccca gaaggcgtcc ttcttccacc ccacggccca cccgagccgg gcctgcaccg
     1261 gggtgatcgt gcgggagatg tcctgcccgt gcagcgggta ccccatctcc gtccgcaacg
     1321 tgtcccgcgc ccccaagccg gcgggcacca gaccgtgctc cgcgccatgg gccatcagcg
     1381 cgtcccagac cgacacggca acggcgttcg gcaccaccag ttcgtagccg cgctcgccgg
     1441 tgtagcccgt ccgacacacc acgaccgggg cgccgtcatg ctccagctcg acgaacgaca
     1501 tgtactcgtg gccggtcggg aatcccaggt ccgcaagaag gtcgtcgctg cgctgaccct
     1561 gcacggcaag gatggcgtag tcacgatgga ggttctcgac cacgatgccc tcaggggcat
     1621 cgtcggcgag ccggcgtacg acctccgtcg tgttggccgc attcgggatc aggaagacct
     1681 cgtcatcgcc acgcagatag gcgatcaggt cgtcgacgac gccgccgtcg tcgacgcagc
     1741 acagcgtgta ctgcgccttc ccgggcgaga tcttgccgag gtcgttggtc aggcaggagt
     1801 tgacgtacgc cgccgcaccc ggtccgtgga ccaccgcctt gccgaggtgg ctcacgtcga
     1861 acaccccgac cgctgatcgg acggcgttgt gctccttcac cgccccggag tactccagtg
     1921 gcatcgacca gccgctgaac tcggcgaact tcgctcccag cgccacatgg cgctcgtgca
     1981 gcaccgacgt gagcaggttg tcggtcgtgt cctcgcccat agttcgcgaa cctatcgcag
     2041 ccggtagagt cgcgggcggg cgatgacgcc gaggaccggg gcagtccatg aggagtaccc
     2101 gagcagaagc gttgaccgaa tcgatgggag cgccgtgatc acgaagtccg ccagcacgtc
     2161 cgccacgttg cgcaacacca gtgcgagaag catcaaagct gatgcggtcg tgatcgggat
     2221 ccacggctcg gcgggcaacg gatccgggat cgccgctgct gcgggcgcgg ccgacgtcgc
     2281 ggacgggttc ggcaagcgct tcgccacgat gctcgaggac ctcggcgcct cgaccaaggt
     2341 cggcgacacc tgcaagcttc ctagcgccga ctcggcgaag gcgccgctgg tggtcacggt
     2401 cggactgggt ccggagccgg acgacgagct cgacctcgaa gcgctccgcc gcgccgccgg
     2461 cgccgccgcc cgcgccgccg ccggcaagaa gaccgtcgtg ctggcactgc ctgcatcgac
     2521 cgacgaccag attcgtgccg ttgtcgaggg cgcactgctg ggcaactacg gatacgaccg
     2581 ttacctgacc aagaagcacg agccggtggc cgagttcgtc gtggtgagcg aggtggcgcg
     2641 gtcgcgaact gcccgaaagg cgctcgccga cgccaccgcg gtcgcggccg cggtgaactt
     2701 ctgccgcgac ctcgtgaacg caccgccgaa cgacctgtat cccgagtcgt tcgccaagca
     2761 ggtcaaggac gccagcaagg gcaccgacgt cacggtcagc atcaccgacg agaaagcact
     2821 cgagtcgaag ggctatggcg gcatcatggg cgtcggccgg ggctcggtcc gtccgccgcg
     2881 cctggtgaag ctgagctacc gcccggccaa accggtggcg cacctggcct tcgtcggcaa
     2941 gggcatcacc ttcgactccg gcggcc
//
LOCUS       DATBFZ010000119         2336 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_2700494, whole genome shotgun sequence.
ACCESSION   DATBFZ010000119 DATBFZ010000000
VERSION     DATBFZ010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2700494"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="VH419_03635"
     CDS             <1..534
                     /locus_tag="VH419_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003951411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY7042740.1"
                     /translation="GASVIAVVREAGPIVTAIVIAGAAGSAICADLGARKIRDEIDAM
                     EVLGISPVQRLVVPRVLGCMLVSVALNGLVCVVGVGGGYFFNVVLQGGTPGAYLASFS
                     ALAQLPDLYAGEVKALIFGLIAGVVASYKGLNADGGPKGVGDAVNQSVVITFLLLFIV
                     NFVITAVYFQIVPAKGG"
     gene            534..1376
                     /locus_tag="VH419_03640"
     CDS             534..1376
                     /locus_tag="VH419_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007078660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY7042741.1"
                     /translation="MASDVLITARRWLTSPVGVLDSLGEQLLFHVRALGWVPRALHRY
                     KKEVLRLLAEVVFGSGALAVIGGTVGVIFVLCFFTGTEVGLQGYAALDQLGIGPYTGF
                     LSAYFNTREIAPLVAALALSATVGCGFTAQLGAMRISEEIDALEVMAVPSLPFLVTTR
                     IVAGFVAVIPLYVLGLLSSYLATYSIEVYYFGQSPGTYSHYFHLFLPPMDVLWSFFKV
                     LVFAVAIILIHCFYGYTATGGPAGVGVAVGRAVRTAIVVVSVVDFFLSLAIWGSTTSV
                     RLAG"
     gene            1380..>2336
                     /locus_tag="VH419_03645"
     CDS             1380..>2336
                     /locus_tag="VH419_03645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HEY7042742.1"
                     /translation="MGLASARRRGFGLLYLVIVAALLWLSIAIYQKKFTHVVLVRLKT
                     DHTGNALQNGSDVKERGIIVGSVRGVHVDSGSEGGCIDQEVTCVTVTLALDPARTRQI
                     PKNVSAQILPKTIFGEQYVALELPAHSDGSIAAGDTIPQDRSRGALETEKVLGDLLPL
                     LQAVQPAELNATLTALATALHGRGAELGQTLVQLDAYLTKLNPHAAQLVQDVEKLGHV
                     ADEYHAAAPDLLSTVDNLQTSARTVITHQQSLAELLDTATDTSDVIRSFLAANEQRLV
                     VLTGSTAKVYALLAHYSPEATCLLGALSKLADATSGIFKDGQM"
BASE COUNT          336 a          783 c          789 g          428 t
ORIGIN      
        1 ggcgcctccg tcatcgctgt cgtacgggag gccggcccca tcgtcacggc catcgtcatc
       61 gccggcgcag ccggatcggc gatctgcgca gacctgggcg cccgcaagat ccgcgacgag
      121 atcgacgcga tggaggtgct cggcatctcg cccgtgcaac ggctcgtcgt cccgcgcgtc
      181 ctcggctgca tgctggtctc cgtcgccctg aacggcctgg tctgtgtggt cggcgtgggc
      241 ggcggctact tcttcaacgt ggtgctgcag ggcggcacgc caggtgcgta tctggccagc
      301 ttctccgcgc tcgcgcaact gcctgacctg tacgcgggcg aggtcaaggc gctgatcttc
      361 ggcctgatcg ccggcgtcgt cgcgtcctac aaggggctca atgcggacgg cggcccgaag
      421 ggtgtgggtg atgccgtcaa tcagtccgtc gtcatcacgt tcctgctgct gttcatcgtc
      481 aacttcgtca tcaccgccgt gtacttccag atcgtcccgg cgaagggcgg ctgatggcga
      541 gcgacgttct gatcaccgcg cggcgttggc tgacgtcgcc ggtcggcgtg ctggactcgc
      601 ttggcgagca gttgcttttc cacgtcaggg cgttgggatg ggtgccgcgc gcgttgcacc
      661 gctacaagaa ggaggtgctg cggctgctcg ccgaggtggt gttcggcagt ggggcgctcg
      721 ccgtgatcgg cggcaccgtc ggcgtcatct tcgtgttgtg cttcttcacc gggacggagg
      781 tcgggctgca gggctatgcg gcgctcgacc agttgggcat cgggccgtac accgggttcc
      841 tgtcggccta cttcaacacc cgcgagatcg cgcccctggt agcggcgctg gcgctgtcgg
      901 cgacggtcgg gtgcgggttc accgcgcagc tcggcgcgat gcggatcagc gaggagatcg
      961 acgcgctcga ggtgatggcg gtgccgagcc tgccgtttct cgtgacgacg cggatcgtcg
     1021 cgggattcgt cgcggttatc ccgctgtacg tgctcggcct gctgtcgtcc tacctggcca
     1081 cgtacagcat cgaggtctac tacttcggtc agtcgccggg cacctactcg cactacttcc
     1141 acctgttcct gccgccgatg gacgtgctgt ggtcgttctt caaggtgctc gtgttcgcgg
     1201 tggcgatcat cctgatccac tgcttctacg gctacaccgc gacgggtggc ccggcgggtg
     1261 tgggcgtggc ggtcggacgc gcggtgcgta ccgcgatcgt ggtggtcagc gtggtcgact
     1321 tcttcctctc cctggcgatc tggggatcca cgacgagcgt tcggctggcg ggctgaccgg
     1381 tgggcctggc atcggcgcgg cggcggggtt tcggcctgct ctacctggtg atcgtcgctg
     1441 cgctgctgtg gctttcgatc gcgatctacc agaagaagtt cacgcatgtc gtactggtca
     1501 ggctgaagac cgatcacacc ggcaacgcgt tgcagaacgg gtcggacgtg aaggagcgcg
     1561 gcatcatcgt cggctcggtg cgcggcgtgc acgtcgactc cggctcggag ggcgggtgca
     1621 tcgaccaaga ggtcacctgc gtcaccgtca cgctcgccct cgacccggcc aggacgaggc
     1681 agatcccgaa gaacgtctcg gcgcagatcc tgccgaagac gatcttcggc gaacagtacg
     1741 tcgccctgga gcttcccgcg cacagcgacg gctccatcgc agcgggcgac accatcccac
     1801 aggaccgatc acgcggcgcg ctggagaccg agaaagttct cggcgacctg ctgccactgc
     1861 tccaggcggt gcaaccggcc gagttgaacg cgacgctcac cgcgctggcc acggccttgc
     1921 acggacgcgg ggccgaactg ggccagacac tcgtccagct cgacgcctat ctgacgaagc
     1981 tgaacccgca cgccgcgcag ctcgtgcagg atgtcgagaa gctcggccac gtcgccgacg
     2041 agtaccacgc cgctgcgccc gacctgctct cgacggtcga caacctgcag acgtccgccc
     2101 gcaccgtgat cacgcaccag cagtcgctcg ccgagctgct cgacaccgcg acagacacgt
     2161 ccgacgtcat ccgcagtttc ctcgccgcca acgagcagcg gctggtcgtg ctcaccggaa
     2221 gcactgcgaa ggtgtacgca ctgctcgcgc actactcgcc cgaagccacc tgcctgctcg
     2281 gcgcgctctc gaagctcgcc gacgcgacgt ccggcatctt caaggacggg cagatg
//
LOCUS       DATBFZ010000120         9494 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_1714774, whole genome shotgun sequence.
ACCESSION   DATBFZ010000120 DATBFZ010000000
VERSION     DATBFZ010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 9494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9494
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1714774"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            331..1041
                     /locus_tag="VH419_03650"
     CDS             331..1041
                     /locus_tag="VH419_03650"
                     /inference="COORDINATES: protein motif:HMM:NF041646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VC0807 family protein"
                     /protein_id="HEY7042743.1"
                     /translation="MTAVLSSQIDVPVERGSLPGADALPKLAAIIRRLATQLLIACVI
                     PGSVFTGLMFLAGLPAALLGALFWSYCAVGWQIAKRCRRSGLLIVTSAVLTVRTLVAL
                     ASGDTLLYFLQPIATDAVISAIFFTSLATSRPMVARLAGDFYPMTEELASRPPIRRLF
                     GRLTALWAMLALIKASVVFWLLQSQSVETFVVAKNAFVFSMNGLAIAVTLGSALFVAR
                     REGLIPSHRAGEEANIRV"
     gene            complement(1403..2356)
                     /locus_tag="VH419_03655"
     CDS             complement(1403..2356)
                     /locus_tag="VH419_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HEY7042744.1"
                     /translation="MTENGSPMYEGRRLPRPDEEVVDQGLGFDLGTLLNRRQLLRFLG
                     IGAAAAGLAACGGGANSSSHPGTSSTSSGEIPDETAGPYPGDGSNGPDVLEQSGIVRS
                     DIRSSFGGATGTAGGVPMTLDLAISDLANGGAPFEGVAVYVWHCSREGGYSMYSDGIQ
                     DQNYLRGVQVTDADGRVRFTSIFPACYSGRWPHIHFEIYPDQASITDATKAIATSQVA
                     MPESVCTKAYAEPGYEASVSNLSQVSLDTDGVFGDDGGVHQLANVSGDVSKGYHVALN
                     VAVDTRTSPSSGDMGGGSGDHDEPPGGPGAPSSSPPTGQSA"
     gene            complement(2580..3881)
                     /locus_tag="VH419_03660"
     CDS             complement(2580..3881)
                     /locus_tag="VH419_03660"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY7042745.1"
                     /translation="METTADVAIIGAGVVGASTALELSRRGRRVVVLDKGGGAGHGST
                     SASSAIVRFNYSTWDGVAASWESKSRWESWRDHLGHGDPAGLARFHRCGLVMLDVETA
                     PRGKSIRLFERAGIPYEEWDAATLRKRLPGIDTGSYYPPKPVTSEGFFSESSHELGGL
                     FTPDAGYVDDPRLAAANLAAAAAQAGATFAYHQQVIGLTRTGETWRIELADDSTVDAP
                     VVVNAAGPWSGAVNRLCDADRDFTVAVRPMRQEVHHVAAPPELSNAAEMPVIADLDLG
                     TYIRPESGQNLLIGGTEPECDPLEWIDDPDASDPRPSLARFEAQVTRAARRFPGLRVP
                     NRPKGISGVYDVASDWTPVYDRTSLPGFYVAMGTSGNQFKNAPLVGALMATLIEGVES
                     GHDHDSDPLTYNCSHTGHVLNIGAFSRKRELNLASSGNVMG"
     gene            3928..4860
                     /locus_tag="VH419_03665"
     CDS             3928..4860
                     /locus_tag="VH419_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042746.1"
                     /translation="MGEHGDGHSLGWVWRRRYAAAGTLVLLASACTGGSSDDPSTSTD
                     SGTASSQPAPVGFHASVQQQRIDVGTRRIGLELTSDHQTTAHVDGVQLLTSAFEQQPA
                     TAKDTEFAPDTTIDLVVTYGAPVCAGDATTHDAQVRVQYDVGGGEQTATLDVDARGRG
                     LLQRLHDTACAKDRLDQAAALSYRLPFQRRQVDGSTALVGALELTRPADGGSGERVTI
                     TSLLGSVLFDFLPLAGGEPTWGRLASRQDTAVIPVEIRSSDLCTQHARSQSQQTFLFS
                     AYVEVGGQPEYRQIVEPPRSLQVQALAMLDDVCG"
     gene            complement(4875..5633)
                     /locus_tag="VH419_03670"
     CDS             complement(4875..5633)
                     /locus_tag="VH419_03670"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEY7042747.1"
                     /translation="MTDTLRHAVHVARHKWPFIDAVEKGHDLPPRLLYAVGWRESNLR
                     NIKGDFSQRSGESSPRFHGFGVWQRDSDAFGVGPEYLKNVHKQATDAADLLVANFRIF
                     DRWDAAVAAYNCGPGNVQKALAEGLSVDHFTANQNYSADVLENRREMMGRHASTDTDH
                     ADPSVVPARRFFRPGQVHSLFTPMGERFIVWLKDDISKAGDRYVPGPEFSTFDRHNVR
                     KCQRLMDDEPDGWFGQGQWTRLFDELPPDVKRRR"
     gene            complement(5800..7077)
                     /locus_tag="VH419_03675"
     CDS             complement(5800..7077)
                     /locus_tag="VH419_03675"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY7042748.1"
                     /translation="MLGVLRRRDFALLWFAGLISTAGDWALFAALPYFVYLQTGSTLA
                     TAGMTAAELAPGILLGTVAGVFIDRWDRKRVLVACNLLQAVTVTALLLVPHGGPLSVV
                     YAVAAVQSSISAFSMPTETAVLPSLVRTDQLLEANAMNALNNRVGRLAGVPAGAVLLG
                     WLGLDWVVFVDSASFLAAALLVTPIVIPSRPEPHEGVLGPAAETESALRALWNEWVQG
                     LRTVGDDRAIAVLFVVFGLMTFGGTMLDPLFVPWVRDILDQGPSVYALLMTTHACAGI
                     LGSLLIGAVGSTLPARALIGWTSVAAGMLLVVRFNVPVVWVAVVLSAIGGMTAVASSV
                     GVETLAQQRVPESMRGRVFGSLQSTIWLLSLLGATVGGILGEAIGIVPALDVAALLTA
                     VAGIVVLVAIPRTAEDTEQGTVYHREAGLPPAQ"
     gene            complement(7077..8258)
                     /locus_tag="VH419_03680"
     CDS             complement(7077..8258)
                     /locus_tag="VH419_03680"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY7042749.1"
                     /translation="MTIPDSPDTLESWPDPTAAAPAWWTAQTRARLVNLCWSITGDPL
                     AGEDLAQETLLEAWRHRERLVSPDGADRWLNAIARNVCRRWARAQRRLGVPCENPEPG
                     MDPGTDLESTLEREELVELLDRALGLLPAATRSALVAHYVEERSHADIARRSGSTAAA
                     VSMRLTRGKARLRFLLETRFADDPLAEAWLRRDEAGWRRTRLRCVSCGRTTTSVRSSP
                     EEIAFRCDTCDPRGLTIRLPLDTPVFGSLVGGLQRPSAVMRRLSAWALDYWTPMQAPD
                     RVRCVRCGRLVAVQPYVRADAWSWSTRRGWYADCDGCGEQVSGSLAGLALALPEVQAA
                     RAREPRLRARPAREVERDGQPAMVLSFGPPDGSETVGAVFLRKSLRLVHVASASPALT
                     G"
     gene            complement(8534..9430)
                     /locus_tag="VH419_03685"
     CDS             complement(8534..9430)
                     /locus_tag="VH419_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008361794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HEY7042750.1"
                     /translation="MSPIFSTIDLSKHLYQADQDHLDDDLEPIASSEDRAERTFDLLL
                     KCVDADPCERARLHEEIVLLNIEVAESIVMRYRNRGVPVEDLVQVACLGLVKAARGFD
                     PVKSDNFLGYAVPTILGEVKRFFRDNAWAVRPPRRIQELQAEIASTSARLMHETGHSP
                     TPHDLAEALDVEVDEVVEALSADGCFAPTSLDRPPRDGETTPLSETLGTDDPGFSHAE
                     AVVALRPLCRGLSERDRRIVYLRFFHEWTQARIAEEFGVTQMQVSRLLTRIFGKLREQ
                     LGPLDMLSPAQRTAAVREADVA"
BASE COUNT         1592 a         3206 c         3217 g         1479 t
ORIGIN      
        1 ccgtgtgccc gaaagggcgt gggggttcaa ctcccccctc gcgcacagag cacttgtagc
       61 gtcggccctg gtcagggcgc gttcgacaac catccggaac gctgctctgg cagagcaggc
      121 cgtgtttggg ccggcgaggc gagggaacgc tcccctcaga atgagtcatc cgccatcacc
      181 cgatcggccg gttctcggcc agagtgacct cgacgacgcg cagcggcacc tacgctcgtg
      241 ggcacgcgaa ggctgtcggg catcgaccca cgacctacga cgcacaccaa gacagcctcg
      301 gcccggtgcc gcggcggacg ggtcccagca gtgacggcag tcctgagtag tcagatcgac
      361 gtcccggtcg agcgtgggag tctgcctgga gccgatgccc ttccgaagct ggccgcgatc
      421 atccgcaggc tcgccaccca actgctcatc gcctgcgtga tcccgggtag cgtcttcacc
      481 ggcctcatgt tcctcgcggg cctcccggcg gccctgctcg gcgcgctctt ctggtcctac
      541 tgcgcggtcg gctggcagat cgcgaagcgg tgccggcgct caggactgct catcgtcacg
      601 tctgcagtgc tgaccgtgcg cacgttggtc gcactggcaa gcggtgacac gctcctctac
      661 ttcctgcagc ccatcgcgac ggatgcagtc atctcagcaa tcttcttcac atcgctggcc
      721 acctcacgac cgatggtggc ccggctcgcc ggagacttct atccgatgac agaggagctg
      781 gcgagcagac ctccgatccg tcggctgttc ggacgactca cggcactgtg ggccatgctc
      841 gctttgatca aggccagtgt cgtgttctgg ctgttgcagt ctcagtccgt cgagacgttc
      901 gtcgtcgcca agaacgcctt cgtcttctcg atgaacggcc ttgccatcgc cgtcacgctc
      961 ggctcggccc tgttcgttgc gcggagggaa ggcctgatcc cgagccaccg ggcaggcgag
     1021 gaagccaaca tccgcgtgtg acccaccagt acgctcgccc tgtcgctgtc ggtgtcgcgg
     1081 tttgcgatgt ttacagccca agaggcgtgt agacatcgca aacctccgat ggggacagcg
     1141 ggtctcggat gtgcacatcg caatcacctc gagccctccc tcgcggcaag ccgacccaac
     1201 gcgccaccca cctgaaccgc gcggacggcg cgcatgcgtg acctccgcca cgagtcaacc
     1261 gaggacggct gagcccggct tttcaccggc tcctggcgac aggccgagta cgggtcgtgg
     1321 gttcatcacc gccggctgga tcctcgtggt catcttcgtt ctgggggtcc tcggccaagc
     1381 tgccaacacc tccacctgat cctcaggcgg actggcccgt cgggggcgag ctgctcggcg
     1441 cgccgggacc accgggcggt tcgtcgtggt cgccggagcc gccgcccatg tcaccactgc
     1501 tgggcgacgt cctggtgtcg accgcgacgt tcaacgcgac gtggtagccc ttggagacgt
     1561 cgccgctgac gttcgcaagc tgatgaaccc caccgtcgtc accgaagacc ccgtcggtgt
     1621 cgaggctcac ttgcgacagg ttgctgaccg acgcctcgta gccgggttcg gcgtacgcct
     1681 tcgtgcagac ggactcgggc atggcgacct gtgaggtggc gatggccttc gtggcgtcgg
     1741 tgatgctggc ctggtccggg tagatctcga agtggatgtg cggccagcga ccggagtagc
     1801 aagccgggaa gatgctggtg aaccgcaccc tgccgtcggc gtcggtaact tggacgccgc
     1861 gcaggtagtt ctggtcctgg atgccatcgg agtacatcga gtagccgccc tcacgactgc
     1921 agtgccagac gtacacggca acgccctcga acggggcacc gccgttggcg aggtcggaga
     1981 ttgcgaggtc gagtgtcatc ggtacgcccc cggccgtccc ggtcgcgcca ccgaagctgg
     2041 accggatgtc gctgcgcacg atcccgctct gctcgagcac gtcaggcccg ttggagccgt
     2101 cacccgggta cgggccggcc gtctcgtcgg ggatctcccc ggacgacgtg gacgacgtgc
     2161 ctgggtgcga cgagctgttc gcgccgccgc cgcaggcagc caggccggct gccgcggcgc
     2221 cgatgccgag gaatcgcagc aactggcggc ggttcagcag cgtgccgagg tcgaagccca
     2281 gaccttggtc gaccacttct tcgtccggac ggggcaggcg tcgaccttcg tacatcggag
     2341 aaccgttctc ggtcatggct gcaggctaga caggagaccc caagaagaac ctgaatgact
     2401 gagcgtccgc tgaggagcca ctgcgaggcg acgctgagga ttcaccgcga attgcgggaa
     2461 agctcaggtt ccgaaggtat ttcgagccgc cagtttcccg caattggggg aacccaaaca
     2521 acagacgaca gttgctgacg ccagcgatac gtcgcgaagg tgaagtggaa gagggtagtt
     2581 caccccatca cgttcccgga cgaggcgagg ttgagctcgc gcttgcggga gaacgctccg
     2641 atgttgagca cgtggccggt gtgggagcag ttgtaggtca gcggatcgct gtcgtggtcg
     2701 tgcccggact cgacaccctc gatgagcgtt gccatcaacg cgccgacgag gggtgcgttc
     2761 ttgaactggt tgccgctcgt gcccatcgcc acatagaacc cgggcagcga agttcggtcg
     2821 tagacgggag tccagtccga cgccacgtcg tagaccccgg agatcccctt gggacggttg
     2881 ggcacgcgca gcccgggaaa tcgccgcgcc gcccgcgtca cctgcgcctc gaagcgggcc
     2941 aggctcggcc gcggatcaga ggcatccggg tcgtcgatcc actccagcgg gtcgcactcc
     3001 ggctcggtac cgccgatgag caggttctgt cctgactccg gacggatgta cgtgcccagg
     3061 tcgaggtcgg cgatcaccgg catctcggca gcgttcgaca actccggggg agcggccacg
     3121 tggtgcacct cctggcgcat cggccgcacc gccaccgtga agtcgcggtc cgcgtcgcag
     3181 agccggttca ccgctccgga ccacggaccg gcggcgttca ccacgaccgg ggcgtccacc
     3241 gtggagtcgt ccgcgagctc gatgcgccat gtctcgccgg ttcgggtcag accgatcacc
     3301 tgctggtggt acgcgaaggt ggctccggcc tgcgccgcag ccgccgcgag gttggccgcg
     3361 gcgagtcgcg ggtcgtcgac gtacccggcg tccggggtga agaggccgcc gagctcatgg
     3421 ctcgactcgc tgaagaaccc ttcgctggtg accggcttcg gcgggtagta cgagccggtg
     3481 tcgatcccgg ggagccgctt gcgcagcgtg gccgcgtccc actcctcgta cgggatgccg
     3541 gcccgttcga agagacggat cgacttgccg cgtggcgccg tctcgacgtc cagcatcacc
     3601 aggccgcacc ggtggaaacg cgccaacccg gcgggatcgc cgtgcccgag gtggtcacgc
     3661 caggactccc agcgggactt cgactcccag gacgcggcca caccgtccca cgtcgagtag
     3721 ttgaaccgga cgatcgcgct cgacgcgctg gtcgagccat ggcccgcgcc gccgcccttg
     3781 tcgagtacga cgactcgacg gccacgccgg ctgagctcga gcgcggtgga ggccccgacc
     3841 acaccggcgc cgatgatggc aacatctgcc gtcgtctcca cacgagcagg ctacggccag
     3901 aggagttgcg tccgtggagg tggccgcgtg ggcgagcacg gcgacggaca tagcctgggc
     3961 tgggtgtggc gacgacggta cgcagcagcc ggaaccctcg tgctgctggc gtcggcatgc
     4021 accggtggct cgtcggacga cccgtcgaca tccaccgaca gcggtacggc gagcagccaa
     4081 ccggcgccgg tcggcttcca cgccagcgtg cagcagcaac gcatcgacgt cggcacccgg
     4141 cgcatcgggc tcgagctgac gagcgaccat cagacgaccg cgcacgtgga cggcgtgcag
     4201 ctgctgacca gtgcgttcga acagcaaccc gccacggcca aggacaccga gttcgcgccg
     4261 gacacgacca tcgacctcgt ggtgacgtac ggcgccccgg tctgtgcggg tgacgcgacg
     4321 acgcacgacg cacaggttcg ggtgcagtac gacgtgggcg gcggcgagca gactgcgacg
     4381 cttgacgtcg atgcccgggg tcgagggctg ctccaacgtc tccacgacac ggcgtgtgca
     4441 aaggacaggc tcgaccaagc cgccgcgctc agctatcggc tgccgtttca gcgacggcag
     4501 gtcgacggca gcaccgctct ggtcggggcc ttggagttga cccggccggc ggacgggggc
     4561 tcgggtgagc gcgtcacgat cacgtcgttg ctcggcagcg tgctgttcga cttcctgccg
     4621 ctggccgggg gcgagccgac gtggggacgg ctggcctcga ggcaggacac cgccgtcata
     4681 cccgtcgaga tccggtccag cgacctgtgc acccagcatg cgcgctcgca gagccagcag
     4741 acgttcctgt tcagtgcgta cgtcgaggtc gggggccagc cggagtaccg ccagatcgtc
     4801 gagccgccga ggtccctgca ggtccaggcg ctggcgatgc tcgacgacgt gtgcgggtga
     4861 cgggactcgg gtgttcagcg acgtcgtttc acgtccggcg gcagctcgtc gaacagccgg
     4921 gtccactgac cctggccgaa ccatccgtcc ggctcgtcat ccatgagtcg ctggcacttg
     4981 cggacgttgt gacggtcgaa cgtcgagaac tccggaccgg ggacgtaacg gtcgcccgcc
     5041 ttgctgatgt cgtccttcaa ccacacgatg aagcgctcgc ccatgggggt gaacagggag
     5101 tggacctggc cgggccggaa gaaccgccgc gcaggcacga ccgagggatc ggcatgatcg
     5161 gtgtcggtcg atgcgtgcct gcccatcatc tcgcgtctgt tctcgaggac atccgcggag
     5221 tagttctggt tcgccgtgaa gtggtcgacc gacaggccct cggcaagcgc cttctgtacg
     5281 ttcccgggtc cgcagttgta cgccgcgacc gccgcgtccc aacgatcgaa gatccggaag
     5341 ttggcgacca gcaggtctgc ggcatctgtg gcctgcttgt ggacgttctt caggtactcc
     5401 gggccgacgc cgaacgcgtc ggagtcccgc tgccacacgc cgaacccatg gaaccggggg
     5461 gacgactcac ccgaacgctg tgagaagtcg cccttgatgt tgcggaggtt ggactcccgc
     5521 cagccgaccg cgtacagcag tcgcgggggc aggtcgtggc ccttctcgac agcgtcgatg
     5581 aacggccact tgtggcgggc gacgtgcaca gcgtgccgca acgtgtcggt catgaacgcg
     5641 cttcccctcc ttggtggcgt cggcaagtct cggcggtcga gcctgtcgag acccaggaga
     5701 gccgaggccc ttgatctcga caagctcgat caacgggctg ctcgacgggt tcgatcagcc
     5761 ggcttctcga ccggttcgat cagcgggcat cgcctctccc tattgtgcgg gaggtagtcc
     5821 agcctcacgg tgatacaccg ttccctgctc ggtgtcctcg gctgtgcggg gaatggcgac
     5881 cagcacgacg atgcccgcga ctgcggtgag cagcgcggcg acgtccagcg ccggcacgat
     5941 gccgatggcc tcgccgagga tgcctccgac cgttgcaccg agaaggctga gcagccagat
     6001 cgtcgactgg agcgaaccga agacccgtcc acgcatcgac tcggggaccc gctgttgcgc
     6061 caacgtttcg acgccgacgg aggaagccac tgctgtcatg ccaccgatgg cgctgaggac
     6121 gacggcaacc cacacgaccg gcacgttgaa tcggacgacc aacaacatcc cagccgcgac
     6181 cgacgtccag ccgatcaacg cacgagcggg cagtgtgctc ccgaccgcgc cgatgagcag
     6241 ggagccgagg attcccgcgc aggcgtgtgt ggtcatcagc agggcgtaga cgctcgggcc
     6301 ctggtcgagg atgtcgcgga cccacggcac gaacagcgga tcgagcatgg ttccgccgaa
     6361 cgtcatcagc ccgaacacca cgaacagaac ggcgatcgcc cgatcgtcac cgacggtgcg
     6421 cagcccctgg acccactcgt tccagagggc tcgcagggca gactcggtct cggcagccgg
     6481 gcccaagacg ccctcgtgcg gctcaggtcg ggacggtatg acgatcggcg tgacgagcag
     6541 cgccgcggcc aggaacgacg cgctgtcgac gaaaaccacc cagtcgaggc cgagccatcc
     6601 gagcaggacg gcacccgcag gcacgcctgc cagtcggccc actcggttgt tgagcgcgtt
     6661 catcgcgttc gcctcgagca gctgatcggt tcggacgagg gagggcagta cagccgtctc
     6721 ggtcggcatc gagaacgccg aaatgctcga ctgcaccgcc gccacggcgt acaccacaga
     6781 cagcggtccg ccgtgcggca ccagcagaag cgccgtcacg gtgacggcct ggagcaggtt
     6841 gcaggcgacc agtacgcgct tgcggtccca gcggtcgatg aacacgccgg caacggttcc
     6901 gagcaggatg cccggcgcga gctcggccgc ggtcatcccg gccgtcgcca acgtcgagcc
     6961 ggtctggagg tacacgaagt acggcagcgc cgcgaacaac gcccagtccc cggccgtcga
     7021 gatcagcccg gcgaaccaga ggagcgcgaa gtcacggcgt cggaggacgc cgagcatcac
     7081 ccggtgaggg caggggatgc agacgcgacg tggacgagac gaagcgactt gcgcaggaag
     7141 acagcaccca cggtctccga accgtccggc ggaccgaagc tcagcaccat ggccggctgc
     7201 ccgtcccgct ccacctcgcg ggctggccgt gcacgcaacc gcggctcacg tgcgcgtgcc
     7261 gcctggacct cgggcagcgc cagagccaga ccggccagcg agccgctcac ctgctctcca
     7321 cagccgtcgc agtcggcgta ccaaccacgt cgggtcgacc agctccaggc gtcggcgcgc
     7381 acatacggtt gcacggcgac cagtcgcccg caacggacgc agcgcacgcg atcgggtgct
     7441 tgcatcggcg tccagtagtc gagcgcccag gccgagagcc gtcgcatgac cgcgctcggc
     7501 cgttgcaggc caccgacgag tgagccgaag accggcgtgt cgagagggag acgaatggtc
     7561 aagccgcgcg gatcgcaggt gtcgcatcgg aacgcgatct cctcgggtga gctgcgcacc
     7621 gaggtcgtcg tacgtccgca ggacacacag cgcagccgag tacgtcgcca gcccgcctcg
     7681 tcgcggcgga gccatgcctc ggccagtggg tcgtcggcga accgcgtctc cagcaggaag
     7741 cgcaaccgtg ccttgccgcg cgtcaaccgc atcgacaccg cagctgccgt cgaaccactc
     7801 cgccgagcga tgtctgcgtg gctgcgttcc tcgacgtagt gtgcgaccaa ggcggatcgg
     7861 gtcgcggcag gaagcaggcc aagggcacga tcgagcagtt cgacgagctc ttcgcgctcc
     7921 agggtcgact cgagatcggt gccggggtcc atgcctggtt ccgggttctc gcacggtacg
     7981 ccgagccggc gctgcgcgcg tgcccagcga cggcagacgt tgcgggcgat cgcgttcagc
     8041 cagcggtctg cgccgtccgg cgaaaccagc cgctcgcgat gccgccacgc ctcgagcagg
     8101 gtctcctggg ccaggtcctc ccccgccagc gggtcgcccg tgatcgacca gcagagattg
     8161 accaggcggg cgcgcgtttg cgcggtccac caggcgggcg cggctgccgt cgggtccggc
     8221 caggactcca gcgtgtccgg gctgtccggg atcgtcacca gtcctccttc gacgagtatg
     8281 tcctggacag atcgcgcgga caccgccgcg ttcaacgctg atagcgcacc gatcggtctg
     8341 gcgccatgac tcgcggccgg gcgactcgcg gccggatgac tcgtactgga agcaggagga
     8401 acgccgcggc gtcgcagccg cgttcatcgg cgccgatcac tccccgactg atcggcccga
     8461 cgatccactc agccgggtca tcgagcctga cgcagacccc ggagtgggcc tgcgacaggc
     8521 tcgaccaccc cactcaggcg acgtcggcct cgcgcactgc ggcggtacgc tgcgccggac
     8581 tcagcatgtc cagcgggccg agctgttcgc ggagcttgcc gaagatgcgc gtcagcagcc
     8641 gtgacacctg catctgtgtc accccgaact cttcggcgat gcgtgcctgt gtccactcgt
     8701 ggaagaaccg caagtacacg atgcgtcggt cgcgctcgga caggcctcga cacagtggtc
     8761 gcagtgccac cacagcctca gcgtgcgaga agccgggatc gtcggtgccg agcgtttcgg
     8821 acaacggagt cgtctcaccg tcgcgcggcg gccggtccaa cgacgtggga gcgaagcaac
     8881 cgtccgccga cagcgcttcg accacctcgt cgacttcgac gtcgagcgcc tcggccaggt
     8941 cgtgcggcgt cggagagtga ccggtctcgt gcatcaaccg cgcagacgtg gaggcgatct
     9001 ctgcttgcaa ctcctggatg cggcgtggcg gacggaccgc ccacgcgttg tcgcggaaga
     9061 agcgcttgac ctcccccaag atggtcggca cggcgtaccc gaggaagttg tcgctcttga
     9121 ccgggtcgaa gccgcgggcg gctttgacca gtccgagaca ggcaacctgg acgagatcct
     9181 cgaccggcac gccgcggttg cggtagcgca tgacgatgga ttcggccacc tcgatgttga
     9241 ggagaacgat ctcttcatgc aggcgcgccc gctcacacgg atcggcgtcg acgcacttca
     9301 ggagaaggtc gaaagtgcgt tcggcccggt cctcggaaga ggcaatgggt tcgaggtcgt
     9361 cgtcgagatg gtcttgatca gcttggtaga gatgcttgga aagatcgatg gtgctgaaaa
     9421 tgggagacac cagaggtcct tcttggagaa cgtcggtggc agttgtctca accacttgcc
     9481 ttcatgcaac catt
//
LOCUS       DATBFZ010000121         3190 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_1388557, whole genome shotgun sequence.
ACCESSION   DATBFZ010000121 DATBFZ010000000
VERSION     DATBFZ010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 3190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3190
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1388557"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(174..380)
                     /locus_tag="VH419_03690"
     CDS             complement(174..380)
                     /locus_tag="VH419_03690"
                     /inference="COORDINATES: protein motif:HMM:NF016828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HEY7042751.1"
                     /translation="MIRQFSSPKRRNKQGASSVEYGLLVALIVAVIVVAFFALDNLFN
                     NSFAGTCSQIKSEGTITTTCPADR"
     gene            complement(555..1208)
                     /locus_tag="VH419_03695"
     CDS             complement(555..1208)
                     /locus_tag="VH419_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017933917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY7042752.1"
                     /translation="MPYTVLLVDDHDLIRQGLRRAFDRNPDFSVTAEASSAGQAIKMA
                     HELEPDVVVCDIRLPDGSGLDVVRQLRERRPDIGLVVLTMYAGDEHLFAALEAGASAF
                     VPKDAPSDDVVAAARHACVSPRSFTAPDLANAMRRRMTPAGPRLSPRETEVLGLLAEG
                     YGVGPIARRLYISESTTKTHISKIYDKLGAGNRAQAIMKAIQSGLITPETTSTAGVN"
     gene            complement(1183..2820)
                     /locus_tag="VH419_03700"
     CDS             complement(1183..2820)
                     /locus_tag="VH419_03700"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEY7042753.1"
                     /translation="MSVTTDGKPALHRARLDIGARSFCLVAGYGLSLVTGTTAEATGV
                     MVLLVAVAGVVSWWAAAEGAGGRAYPMVLVAEATFATTLIASAGARADSYFIYLAAPP
                     LLAGLRRGLTGLLAVTAAETLTLVLLQAQFASHADPLQRGGPWVLTGFAVGLLGAWIL
                     QLHLLERETRLSPYASAHRLLGQLRTIVRDLPGGLDTEVVSENILSTAVAALAGNRAA
                     LLVQNESDQPMIVAHRGDDAFISTLASDPLITTSMGVRRAMQQPDPHEQASHGFRVCI
                     PMVVGHRSIGAVVVDGPNAVSDEVLESVQESLYDEALRLESAALFSDVKAMATVEERH
                     RLAREIHDGIAQEIASMGYLVDDLAQDECDQRHQEGLVTVREELTRVVTELRHSIFDL
                     RSGVSSSVGLGSVLGDYVREVGKDSGMTVHLSLEESPRRLRLGVETELLRIAQEAVTN
                     ARKHSQAENMWVACRVEPPFAELRIEDDGIGSVAHRTDHFGLHIMKERAARINAVLTI
                     GDRVGGGTVVSAVLLPAEAAAVSTPNGETHALHRFAS"
BASE COUNT          553 a          990 c         1104 g          543 t
ORIGIN      
        1 ggcgattcag gcggtgccgc gcagaccgcg cagtccattg gtgggactgt ggtcgaccct
       61 ctgtcgtcgt tcgccgtcgc gaccgagatt gtgagaagac tcgacggtcg aggccagcgc
      121 gcaggaccga tgcgaacgta atggccaccg cagttcctgg cccacggcga gtgtcacctg
      181 tcagcgggac acgtggtggt gatcgtgcct tcgctcttga tctgggagca ggtcccggcg
      241 aacgagttgt tgaaaagatt gtcgagggcg aagaaggcga ccacgatgac agcaacgatg
      301 agtgccacca gcaggccgta ctccacggac gaggcgcctt gcttgttgcg acgcttcggg
      361 ctggagaact ggcggatcat ctcgagcatt ggtctgttgc ccccgggact tcttgcgagt
      421 cgctggagac acttccagct catcggcaat ggtcccggat ttgcgtttcg tccgatatcg
      481 ggcgagcggc caccacccgt tgaccgaacg gctcatgggg aacaccagac tctcggcctg
      541 cgggagtatg gggactagtt cacgcccgcc gtcgacgtcg tctccggcgt gatgaggccg
      601 gactggatcg ccttcatgat cgcctgcgca cggttgcccg caccgagctt gtcgtagatc
      661 ttcgagatgt gggtcttcgt cgtggactcg ctgatgtaga gccgacgggc gatcggcccc
      721 acgccatagc cctcggccag gagacccaac acctcggttt cacgcggcga cagacggggg
      781 cctgccggtg tcatccgccg gcgcattgca ttggccaggt cgggcgcggt gaacgagcgg
      841 ggtgacacgc aagcgtgacg agccgcagcc accacatcat cgctcggcgc gtccttgggc
      901 acgaaggcgg aggcgcccgc ctcgagagcg gcgaacaggt gctcgtcgcc ggcgtacatg
      961 gtgaggacca cgaggccgat gtcaggtcgg cgctcacgaa gctgacgcac gacgtcgagt
     1021 ccgctgccgt cggggagccg gatgtcgcag accacgacgt caggctccag ctcgtgagcc
     1081 atcttgatcg cctggccggc tgacgacgcc tcggcggtca cgctgaagtc ggggttacgg
     1141 tcgaacgcgc gccggagtcc ttggcggatc aggtcgtgat cgtcaactag caaaacggtg
     1201 tagggcatgg gtctctccgt tgggggtgga tactgctgct gcttccgcgg ggagcaggac
     1261 tgcggaaacg acggtgccgc caccgacgcg gtcgccgatg gtgaggacgg cattgatccg
     1321 ggccgcgcgc tctttcatga tgtgcagccc gaagtgatcg gtccggtgag cgaccgatcc
     1381 gatgccgtcg tcctcgatgc ggagttcggc aaaaggtggc tcgacgcgac aggcgaccca
     1441 catgttctcg gcctgtgagt gcttgcgtgc gttggtgacg gcctcttggg cgatgcggag
     1501 caactcggtc tccacaccga gacgcagccg tcgaggactc tcctccaaag acagatgcac
     1561 ggtcataccg gagtccttgc cgacctctcg cacgtagtcg ccaaggacgg agccgagtcc
     1621 aacgctggac gagacgccac tgcggaggtc gaaaatcgag tggcgcaact ccgtgacgac
     1681 ccgggtcagc tcctcgcgca cggtgaccag accctcttgg tgtcgctggt cgcactcgtc
     1741 ttgggccagg tcgtcgacca gatagcccat cgacgcgatc tcctgcgcga tgccgtcgtg
     1801 gatctcccgg gcgagccggt gtcgttcctc gaccgttgcc atcgccttga cgtcgctgaa
     1861 cagtgccgcc gactcgaggc gcagcgcctc gtcatacaac gactcctgga ccgactcgag
     1921 tacctcgtcg gacactgcgt tcggaccgtc gacgacgacg gcgccgatgg atcgatgacc
     1981 gacgaccatg gggatacaga cgcggaagcc atggctcgcc tgctcgtgcg gatctggctg
     2041 ctgcatggcc cgccgcacgc ccatggacgt ggtgatcagc gggtcggagg ccagagtcga
     2101 gatgaaggcg tcatcgcctc ggtgcgcgac gatcatcggc tgatcggact cattttggac
     2161 cagcaacgct gcccggttgc cggccagcgc ggcgacggcc gtggacagga tgttctcgga
     2221 caccacttcg gtgtcgagac cgcctggcag gtctcgcacg atcgtccgga gctgcccgag
     2281 caggcggtgg gcagatgcgt acggcgagag gcgcgtttcc cgctccaaga ggtgcagctg
     2341 caggatccag gcgccgagca ggccgacagc gaacccggtc aggacccacg ggccaccacg
     2401 ctggagcggg tcagcgtggc tggcgaactg tgcctggagc agcaccaagg tcagcgtttc
     2461 tgctgccgtg actgccaaca agccggtgag cccccgccgc aacccggcga gcagtggcgg
     2521 agcggccaga tagatgaagt acgagtcggc tcgcgcgccg gcggacgcga tgagcgtcgt
     2581 ggcgaaggtc gcttctgcaa ccagaaccat gggataggcc cgcccccctg caccttccgc
     2641 ggcagcccac catgaaacga cgccggcgac ggcaacaagc agcaccatga cgccggtggc
     2701 ctccgccgtc gtgccagtga ccaacgacaa cccgtatccg gccaccagac agaagcttcg
     2761 agctccgatg tcgaggcggg cgcggtgcag cgcaggcttc ccgtcggtgg tcacggacat
     2821 ggcgtcgagg gtgacatgga gtgaggacta gtacccatgg ttcgccgaga aatagtcacc
     2881 atgggctatg gaaatctgtg tcgcgccctg cggcgggggc gaggtgggtt gcctggtcgg
     2941 ccggagctgg catctgtgcc ttccttggag cggcagcgac gttcgggggc ttgtcagcta
     3001 gagagacggg agggcctcgc ggtccttacg gccccagggg tggtgagttg ggtcacaatt
     3061 cgataacgac cagacatcgt tggggttggg agacacgtct cttgggtgtt cagaggtcgt
     3121 ccaggtagcg caggtcgcgg agcagcgcct ctcggccctc gaggacgacc ttggccggct
     3181 cgggctccgg
//
LOCUS       DATBFZ010000122         4280 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_3030804, whole genome shotgun sequence.
ACCESSION   DATBFZ010000122 DATBFZ010000000
VERSION     DATBFZ010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 4280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4280
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3030804"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..773
                     /locus_tag="VH419_03705"
     CDS             <1..773
                     /locus_tag="VH419_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017936704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEY7042754.1"
                     /translation="RLTTPDDKTFVGLSNYGTVLTDSLFWQDTLNTVIIMVVTVAIEL
                     VIGFAFAMVMHRIVFARGAIRTSILIPYGIITVVSAYAWQFAFSLNNGWVNSWFDWLP
                     WITADTNWFGSHWPAMFAIMVSEIWKTTPFMSLLLLAGLSQVSEDMIEAAKVDGATWW
                     QRLWKVILPNMRAAIMVAVLFRALDAFRIFDNIYVMTRGAQDTESSSFLTYRQVIQQF
                     QLGLGSALSVLLFLTVLLLAWLIVKLFRVDLAQARGEA"
     gene            773..1639
                     /locus_tag="VH419_03710"
     CDS             773..1639
                     /locus_tag="VH419_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017936703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEY7042755.1"
                     /translation="MHETRNKIGTWIGFIIILVWCLLPVVWIMSLSFKGPGETAAGSP
                     QFLPKDWTFQNYRDILGIGTVTPGQENTNNDFLAALRNSFGIAAIATVLSVIFATLAA
                     YAIARLDFKGKRLVLSLALAIAMFPVVALVGPLFDLWRTLGLFNTWPGLIIPYMSFTL
                     PLAIWTLSAFFREIPWEMEQAAQVDGATSWQAFRKVIVPLAAPGVFTAAILTFFFAWN
                     EFVLALSLTSTTKARTVPAQMSFFVGPDPFNPPYSQLATAAVIVTIPIIIIVLLFQRR
                     IVAGLTSGAVKG"
     gene            1646..2863
                     /gene="ugpC"
                     /locus_tag="VH419_03715"
     CDS             1646..2863
                     /gene="ugpC"
                     /locus_tag="VH419_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017936702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEY7042756.1"
                     /translation="MAAIEMKNIVKQYGDGFAAVNDVTIDVADGEFMILVGPSGCGKS
                     TLLRMIVGLEDITSGDMVIGGERVNEKAPRDRDLAMVFQNYALYPHLSVYENIAFPLR
                     LSKQFNDQQIDEKVREASRTLELDEHLDRKPGNLSGGQRQRVAMGRAIVRDAKAFLFD
                     EPLSNLDAKLRGQMRTEIARLQKRLGITTVYVTHDQTEAMTLGDRVAVLKRGLLQQLA
                     TPRELYEHPVNLFVAGFIGSPPMNFMPATVEGDTVELPFGTMKLPREKAERAQGKGLL
                     MAGVRPEYFDDAATMDRSKVGEGSTFRATVDVVEWLGNEQYAYIPFEAPPEVQEQLVQ
                     LAKDTDAEAVHTQLIVSLDSSSRVTAGEELELYVHTEKMHLFDPATGENLTVDSSEAG
                     QLGKHALEDEPQS"
     gene            3035..4117
                     /gene="ugpC"
                     /locus_tag="VH419_03720"
     CDS             3035..4117
                     /gene="ugpC"
                     /locus_tag="VH419_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEY7042757.1"
                     /translation="MATVTFEKAQRWYPGTDAPAVPALDLEVKDGEFMVLVGPSGCGK
                     STTLRMLAGLEEVNSGRVYIGDRDVTNVPPKDRDIAMVFQNYALYPHMTVADNMAFAL
                     KMAKVPSAERRRRVEQAAKLLGLTEYLQRKPKALSGGQRQRVAMGRAIVREPQVFCMD
                     EPLSNLDAKMRVQTRTDIAKLQRDLGTTTVYVTHDQVEAMTMGDRVAVMQLGVIQQVD
                     SPLELYDKPVNLFVAGFIGSPQMNLMEATAVDGVASIDSYTVPVDPAAAARMKGKITV
                     GVRPEAWRMVTREDGGLPIDVTVVEELGSDSFVYGTCAVEGTPRDIIVRVGTRDTVRR
                     GDTIYVTTDPDSVHVFDTESGERLSD"
BASE COUNT          739 a         1416 c         1396 g          729 t
ORIGIN      
        1 accggctgac cacgccggac gacaagacgt tcgtcgggct gtccaactac gggaccgtgc
       61 tgaccgactc gctgttctgg caggacaccc tcaacaccgt gatcatcatg gtcgtcaccg
      121 tggcgatcga gctcgtgatc gggttcgcgt tcgcgatggt gatgcaccgg atcgtcttcg
      181 cccgcggggc catcaggacc tcgatcctga tcccctacgg gatcatcacc gtggtctcgg
      241 cgtacgcgtg gcagttcgcg ttcagcctca acaacggctg ggtgaacagc tggttcgact
      301 ggctgccgtg gatcaccgcc gacaccaact ggttcggcag ccactggccg gcgatgttcg
      361 cgatcatggt gtcggagatc tggaagacca cgccgttcat gtcgctgctc ctgctggccg
      421 ggctctcaca ggtctcggag gacatgatcg aggccgccaa ggtcgacggc gccacctggt
      481 ggcagcggct gtggaaggtg atcctcccga acatgcgggc cgcgatcatg gtcgcggtgc
      541 tgttccgggc cctggacgcc ttccggatct tcgacaacat ctacgtgatg acgcgaggtg
      601 cgcaggacac cgagtcctcg tcgttcctga cctaccgcca ggtgattcag cagttccagc
      661 tcggtctggg ctcggcgctc tcggtgctgc tgttcctcac cgtgctgctg ctggcctggc
      721 tgatcgtcaa gctgttccgg gtcgacctgg cgcaggcgag gggtgaggcg tgatgcacga
      781 gacgcgcaac aagatcggca cctggatcgg gttcatcatc atcctggtct ggtgcctgct
      841 cccggtcgtg tggatcatgt cgctgtcctt caagggcccc ggcgagaccg cggccggcag
      901 cccgcagttc ctgcccaagg actggacctt ccagaactat cgcgacatcc tcgggatcgg
      961 caccgtcacg ccgggccagg agaacaccaa caacgacttc ctcgccgcgc tgcgcaactc
     1021 cttcgggatc gcggccatcg cgacggtgct gtcggtgatc ttcgcgaccc tggcggccta
     1081 cgccatcgct cgcctggact tcaagggcaa gcgtctggtc ctctcgctcg cgctggcgat
     1141 cgcgatgttc ccggtggtgg cgctggtggg cccgctgttc gacctctggc ggacgctcgg
     1201 cctcttcaac acctggcccg ggctgatcat cccgtacatg tcgttcacgc tgccgctggc
     1261 gatctggacg ctgtcggcgt tcttccgtga gatcccgtgg gagatggagc aggcggccca
     1321 ggtcgacggc gcgacgtcgt ggcaggcgtt ccgcaaggtg atcgtcccgc tggcggcgcc
     1381 cggcgtcttc accgccgcga tcctgacgtt cttcttcgcc tggaacgagt tcgtgctcgc
     1441 gctctccctc acgtccacaa cgaaagcgcg aaccgtgccg gcacagatgt cgttcttcgt
     1501 cggccccgac ccgttcaacc ctccctactc acagctcgcg acagcggctg tgatcgtgac
     1561 catccccatc atcatcatcg tgctcttatt ccaacgtagg atcgtcgccg gtctcacctc
     1621 cggcgcagtg aaggggtgac gccagatggc agccatcgag atgaagaaca tcgtcaagca
     1681 gtacggcgac ggatttgctg cggtcaacga cgtgaccatc gacgtggccg acggggagtt
     1741 catgattctc gtcgggccgt ccggttgcgg gaagtccacc ctgctccgga tgatcgtcgg
     1801 cctcgaggac atcacgagtg gtgacatggt gatcggtggc gagcgcgtga acgagaaggc
     1861 gccgcgcgac cgcgacctgg cgatggtgtt ccagaactac gcgctgtacc cgcacctgtc
     1921 cgtctacgag aacatcgcgt tcccgctccg gctgtcgaag cagttcaacg accagcagat
     1981 cgacgagaag gtccgtgagg cgagccggac actcgagctg gatgagcact tggaccgcaa
     2041 gcccggcaac ctttcgggtg gccagcggca gcgggtggcg atggggcgcg cgatcgtacg
     2101 cgatgcgaag gccttcctct tcgacgagcc gctttcgaac ctcgacgcca agctgcgtgg
     2161 gcagatgcgt acggagatcg cccggttgca gaagcgactg ggcatcacca cggtctacgt
     2221 cacgcacgac cagaccgagg ccatgacgct cggtgacagg gttgccgtcc tgaaacgcgg
     2281 cctgctccag cagctggcga cgccccgcga gctgtacgaa cacccggtca acctgttcgt
     2341 cgccggtttc atcgggtcac caccgatgaa cttcatgccg gcgacggtcg agggggacac
     2401 ggtcgagctc cccttcggca cgatgaagct gcccagggag aaggcagagc gagcccaggg
     2461 caagggcctg ctcatggccg gggtgcggcc ggagtacttc gacgatgcgg cgaccatgga
     2521 tcgcagcaag gtcggcgagg gatcgacctt ccgggccacc gtcgacgtgg tcgagtggct
     2581 gggcaacgag cagtacgcct acatcccgtt cgaggcgccg ccagaggtgc aggagcagct
     2641 cgtgcagctt gccaaggaca ccgacgccga ggcagtgcac acgcagctca tcgtctcgct
     2701 cgactcctcg agcagggtga cggcaggcga ggagctcgag ctctacgtgc acaccgagaa
     2761 gatgcacctg ttcgaccccg ccaccggcga gaacctcacc gtcgactcga gcgaggccgg
     2821 ccagctgggc aagcacgctc tggaggacga gccacagtcc tagtgtccac ccgattcgcg
     2881 agccacttcg agcccacccg caccatgggc gcctgacgtc gcgtacattg cccgagtcac
     2941 cgcggtcgac agtcctgtcg atccgcgcgg accctggtca ccttttacca cggatcgtcc
     3001 ggcacgtgcc tgccggtgaa aggaacgcct cgtcatggca actgtgacgt tcgagaaagc
     3061 tcagcggtgg tacccgggta ccgacgcacc ggccgtgccg gccctcgacc tcgaggtcaa
     3121 ggacggcgag ttcatggtcc ttgtcgggcc gtccggctgc ggcaaatcca cgaccctccg
     3181 catgctcgcc ggcctcgagg aggtcaattc gggcagggtc tacatcggtg atcgggacgt
     3241 gacgaacgtc cctccgaagg accgtgacat cgcaatggtc ttccagaact acgcgctgta
     3301 cccccacatg accgtcgccg acaacatggc cttcgcgttg aagatggcaa aggtcccgag
     3361 tgccgaacgt cgcaggcggg tcgagcaggc cgcgaagctg ctcggtctga cggagtacct
     3421 ccagcgcaag ccgaaggcgc tgtcgggcgg ccagcggcag cgggtggcga tggggcgggc
     3481 gatcgtgcgc gagccgcagg tgttctgcat ggacgaaccg ctgtcgaacc tcgacgcgaa
     3541 gatgcgggtc cagacccgca ccgacatcgc caaactgcag cgcgacctcg gtacgacgac
     3601 cgtgtatgtc acccacgacc aggtcgaggc catgacgatg ggggaccggg tggccgtgat
     3661 gcagctcggg gtcatccagc aggtcgacag cccgctcgag ctctacgaca agcccgtgaa
     3721 cctgttcgtc gccgggttca tcggttcgcc gcagatgaac ctgatggagg cgacggcggt
     3781 tgacggagtg gcgtcaatcg acagctacac cgtgccggtc gaccctgctg ctgccgcgcg
     3841 gatgaagggc aagatcaccg tcggcgtccg ccccgaggcg tggcgcatgg tcaccaggga
     3901 ggacggtggc ctgccgatcg acgtcaccgt cgtcgaggaa ctcgggtcgg acagcttcgt
     3961 ctacgggacc tgcgcggtcg aagggacccc gcgcgacatc atcgtccgcg tcgggacgcg
     4021 ggacaccgta cgccgcggcg acacgatcta cgtcaccacc gatccggaca gcgtccacgt
     4081 cttcgacacc gagtccggtg agcgcctcag cgactgacct cgaccgtcga acacgccgcc
     4141 cgctgatcga gccgaccggg gtctcgacaa gctcgaccgc cggggtgtcc gctgatcgag
     4201 ccgaccgggg tgcccgctga tcgagcctgt cgagatcgag ggccgcaacc gaccggggtc
     4261 tcgacaagct cgaccgccgg
//
LOCUS       DATBFZ010000123         3163 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_1063433, whole genome shotgun sequence.
ACCESSION   DATBFZ010000123 DATBFZ010000000
VERSION     DATBFZ010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 3163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3163
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1063433"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="VH419_03725"
     CDS             complement(<1..194)
                     /locus_tag="VH419_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042758.1"
                     /translation="MSEYILRDRAAVIGIGMTEFSSDSGVSELTAAVRAAKLACEDAG
                     VDPKAVDGFGSYNSYGEGPNA"
     gene            439..2067
                     /locus_tag="VH419_03730"
     CDS             439..2067
                     /locus_tag="VH419_03730"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY7042759.1"
                     /translation="MHSGDDVDRRFPSRDACVLEPLLRRNARDRPNQLFVQFEDGSAW
                     TYRQAEDAALRAAAAFQALGVGPRDRVLSWLPNGPDALRVWFGSNLCGAVYTPVNIAY
                     RGQLLEHVIRNSGARTMVVHAELLPRLCEADLGGLELVIVAGGDAAPAAGARCLPVSV
                     LEGTGLTFIPPADPVQPWDDYAVIYTSGTTGPSKGVRSTYIQLYEGVRAAYIGCGVTA
                     DDRYLLQVPLFHLGGIIGVYAMAVVGGSVVMVSAFDTARFWTVVGEHGITVCTLLGAM
                     ASFLERRSPQPSDANNPLRLVFMVPLVRDVLGFARRFGVELRTMFSMTEVPPPLISEP
                     KSTQLGSCGRLRDGLHARIVDTHDREVPTGETGELILRSDLPWSITHGYLGAPDATAA
                     AWRNGWFHTGDAFRRDGAANYYFVDRIKDYIRRRGENISSFELEAEVLAHPAVREVAA
                     VAVPSEDLEDEILVVAAPKTGAAIDPAELIGFLSTRVPHFMVPRYVRLVDALPKTPTA
                     KVRKQVLRDEGVTTDTFDREQAAIRVKRDRIGER"
     gene            complement(2290..>3163)
                     /locus_tag="VH419_03735"
     CDS             complement(2290..>3163)
                     /locus_tag="VH419_03735"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEY7042760.1"
                     /translation="PGPPEVLTDVEILAEGLVTPEGPVPMADGSILLVEIDRGTLSRV
                     RAGTTEVVAYCGGGPNGLAIGPDGAAYICNNGGVLPHYSGGRIERIDLESSEVQILYR
                     EYDGEQLSAPNDLVFDDKGGFWFTDSGARRRRSRDYGRIFYAKVDGSELRLVLDRVSL
                     PNGIGLSPDGDTLYFVETYSARLYRRTITGMGEVEHARVDDPETLLCGLPGLQWFDSL
                     AIDSSGNICIATMHSGCITAVSPNGSTVRQFLLPPHAYDPLPTNICFEGTEEQRAYIT
                     LSSTGRLARCAWST"
BASE COUNT          548 a         1032 c          999 g          584 t
ORIGIN      
        1 gcgttcggtc cttcaccata cgagttgtag ctgccgaagc cgtccaccgc tttgggatcg
       61 actccggcat cttcgcaagc aagcttggcc gctctgaccg ccgcggtcag ctcgctcacg
      121 cccgagtcac tggagaactc cgtcatcccg atcccgatga ccgccgctcg gtccctcagg
      181 atgtactcgg acatcgcgcc tcctcgtgac cggagccgtc gagtggatca ccacgcacga
      241 gcgggcagtg gatcgagtgc tccattcaac ttctccatct cgggccagtc aaggttcgag
      301 cgacggtcgc cactcggtcg caggcttgac aggtcgtcga cccaacggct tgattggagc
      361 gcctgcacca gaggatcgcg tgcggagcga ggtgtccgtg ccgatcacgc ctcgacgtcg
      421 acgagcaggg ggcaacgcgt gcacagcggt gacgacgtgg accgtcggtt cccctcgcga
      481 gacgcctgcg tactggaacc actcttgcgg cgaaacgccc gggaccgacc gaaccagctc
      541 ttcgtacagt ttgaggacgg ttcggcgtgg acctatcgcc aggccgagga tgccgcgctg
      601 cgggcagccg cggcgttcca ggcgctcggg gtgggccccc gcgaccgggt gttgtcgtgg
      661 cttcccaacg gtccggacgc tcttcgcgtg tggttcgggt ccaacctctg tggcgccgtc
      721 tacacgccgg tcaacatcgc ctatcgcggc cagctgctcg aacacgtgat ccgcaactcg
      781 ggtgcgcgga cgatggtggt gcacgccgag ctcctacccc gcctgtgcga ggcagacctc
      841 ggtgggctcg agctcgtcat cgtggccgga ggtgatgccg ccccggccgc cggagcccgc
      901 tgcctaccgg tctccgtgct tgaggggacg ggtctgacgt tcatcccacc ggctgatccc
      961 gtacagccgt gggacgacta cgccgtcatc tacacgtcag gcacgacggg gccgtcgaag
     1021 ggcgtgcggt ccacgtacat ccagctttac gagggcgtgc gggccgcgta catcgggtgt
     1081 ggggtcactg ccgacgatcg ctacctgctg caagtccctc tcttccacct cggcggcatc
     1141 atcggcgtct acgcgatggc cgtcgttggc gggtccgtcg tcatggtctc ggcgttcgac
     1201 acggcgcggt tctggaccgt cgtcggtgag cacgggatca cggtgtgcac gctcctgggc
     1261 gccatggcga gcttcctcga acgccggtca ccccagccga gcgatgccaa caacccgctg
     1321 cggctcgtct tcatggtgcc gctcgtcagg gacgttctcg ggttcgcgcg tcggttcggc
     1381 gtggagctgc gcacgatgtt cagcatgacc gaggtcccgc caccactgat ctcggagccc
     1441 aagagcacgc agctcggctc gtgcggccgg ctgcgcgacg gcctccacgc tcggatcgtc
     1501 gacacgcatg atcgggaggt gcccacgggt gagacgggcg agctgatctt gcgatcagac
     1561 ctgccgtggt cgatcacgca cggctacctg ggcgctcccg atgcgacggc ggcggcgtgg
     1621 cgcaacggtt ggttccacac cggcgacgcc ttccggcggg atggggccgc gaactactac
     1681 ttcgtggacc gcatcaagga ctacatccgg cggcggggtg agaacatctc ctcgttcgag
     1741 ttggaagcag aggtgctggc ccacccggct gttcgagaag tcgccgcggt cgccgtgccc
     1801 tcggaggacc tggaagacga gatcctcgtc gtcgctgctc ccaagaccgg tgcggccatc
     1861 gatccggccg agctcatcgg cttcctctcg acacgggttc ctcatttcat ggttcctcgt
     1921 tacgttcggc tcgtggacgc gctaccgaag acgccgacgg ccaaggtgcg gaagcaggtg
     1981 ctccgggacg agggtgtcac caccgacaca ttcgatcgtg aacaggccgc catccgggtc
     2041 aaacgagacc ggatcggaga gagatgagct ggccgcacag agtcctggag gaacgcgtcg
     2101 actcgcgaac cgtgtcgaca gccgacggtc tcaatcttcc ggcccgcagc ttccctccag
     2161 agcctcccca ggtcggggct ccggagcaat cgagggccag acccgggttg agctggtgag
     2221 gatgctactg atcgcgtgcc cagcgagtct catcgtctcc aggatcgtcc tgactcgagg
     2281 gtgcgccgct caggtgctcc atgcgcaccg cgcgagccgt ccggtcgaag aaagggtgat
     2341 gtatgccctc tgttcttcgg tgccttcgaa gcagatgttc gtaggtaatg gatcgtacgc
     2401 gtgcggtggc aggagaaact gcctcaccgt cgacccgttt ggtgatacgg cagtgataca
     2461 tccgctatgc atggtagcga tgcagatgtt gcctgatgag tcaattgcga gcgaatcgaa
     2521 ccattgcagg cccggcaatc cacagagaag tgtctcagga tcgtcgacgc gcgcgtgttc
     2581 tacctcgccc ataccggtga tcgttctacg ataaaggcgt gccgaatagg tctcgacgaa
     2641 gtacagcgta tcgccatcag gagacaagcc gataccatta ggcaggctca ctcgatccaa
     2701 taccaatcgc agctcagagc catcgacctt agcatagaag atccgaccgt agtcacggct
     2761 gcgccgtctg cgcgcaccac tgtccgtgaa ccaaaacccg cctttgtcgt cgaaaacgag
     2821 gtcgttcgga gcggacaatt gctcgccatc gtactctcga tagagaatct gaacctcact
     2881 agactctagg tcgattcgct cgattcggcc accggagtag tggggcaata cgccgccatt
     2941 attgcaaatg tacgcggcac cgtccggtcc tatcgccagg ccattcggac caccgccgca
     3001 gtaggcgacg acctcagttg tgccggcgcg cacccgagac aacgtgcctc gatctatttc
     3061 aacgaggaga atgctgccat cagccattgg gaccggtccc tccggcgtga caagcccttc
     3121 tgccagaatc tcgacgtccg tcagcacttc gggtggccct ggc
//
LOCUS       DATBFZ010000124         3016 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_3359175, whole genome shotgun sequence.
ACCESSION   DATBFZ010000124 DATBFZ010000000
VERSION     DATBFZ010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 3016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3016
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3359175"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="VH419_03740"
     CDS             <1..218
                     /locus_tag="VH419_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020579502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042761.1"
                     /translation="TGEAVVAYVVPAPDADRVADAVRDHCAVRLARFKQPSRVEVVDE
                     LPLTVTGKVQKGRLRGIERRRSLGLLE"
     gene            250..498
                     /locus_tag="VH419_03745"
     CDS             250..498
                     /locus_tag="VH419_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020388028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HEY7042762.1"
                     /translation="MSDPRITLYSRPGCHLCDDARVLIEQICAELGESYAEVSVADDP
                     DLERRFGHEVPVTFVDGRQHDYWRVDPGRLRAALTARG"
     gene            1416..1646
                     /locus_tag="VH419_03750"
     CDS             1416..1646
                     /locus_tag="VH419_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042763.1"
                     /translation="MPSVQIKDVPAETHAVLRQRAAAAHQSLQEYLRARLIDEASRPT
                     LDEVMDRAGGRAGGSVPLKAAASTLRAERARR"
     gene            1633..2025
                     /locus_tag="VH419_03755"
     CDS             1633..2025
                     /locus_tag="VH419_03755"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEY7042764.1"
                     /translation="MLVVDASVLATALADDADDGDRARSRLRGEAVAAPDLVDLEVTS
                     VLRGRLAGGHISPRRAELALTDLVALPLTRSPSLPLIPRCWELRDNLTVYDAAYVALA
                     EALTVPLLTADARVAKATGPRCPIEVLR"
     gene            complement(2078..2851)
                     /locus_tag="VH419_03760"
     CDS             complement(2078..2851)
                     /locus_tag="VH419_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042765.1"
                     /translation="MWTREQVLGASHAWQWVPPGAEELHIHDVVVIDYPDWARMGFYA
                     MPEHVTDSDQTVAAVCEAARSRGRTSTEWWVTPSTSDALERTLIQRGAVATDAADILA
                     YDMSGGVPAVPVPDDVHAVVVTDAQSLDDAETVAAAVWGGEPSSNKRRDAQLRSLGDP
                     RDEHGGFRVVAYADRTAFATAGCQVVDGVARLYGGCVLPEMRGRGGYRQTLLTRLEVA
                     HDHSARLALVHARVNTSKPILTRLGFNTYGEGRLYTLTA"
BASE COUNT          469 a         1027 c         1001 g          519 t
ORIGIN      
        1 tgaccggcga ggcggtcgtc gcgtacgtcg taccggctcc ggatgccgac cgggtcgccg
       61 acgccgtgcg cgaccactgc gccgtacgac tggcgcgctt caagcagccc tctcgcgtcg
      121 aggtcgtcga cgagctcccg ctgaccgtca ccggcaaggt gcagaagggg cgactccgag
      181 gcatcgaacg ccggcgctcc ctgggactcc tggagtgagg ccgctgggct cggctggtga
      241 gatggggtcg tgagcgaccc ccgcatcacc ctgtacagcc gcccgggctg ccacctctgc
      301 gacgacgccc gcgtgctcat cgagcagatc tgcgccgagc tcggagagtc ctacgccgag
      361 gtctccgtcg ccgacgaccc cgacctcgaa cgccgcttcg ggcacgaggt cccggtgacc
      421 ttcgtggacg gacgccagca cgactactgg cgcgtcgacc ccgggcgcct ccgcgccgct
      481 ctcacggccc gaggctgacg cggtccgcca gtcgagagtg cagtacgtgg cgtcaaccac
      541 ccagcttcgg cgggtaaggg cgctgctcca tcccaccagg gccggtgccc gggccgtcga
      601 actcacgaac tgccccgcaa acgctgcagg tgcggactgt cccagggcct ccgtcggccg
      661 cgtacggacc cagcggtgta ttcgaagacg acacagaagc cgcatcggcg gactcctttc
      721 aacccccgca cagaccctgc cgatcaagga tggcatgcgt gggcgccctc acgcgccgag
      781 tgagcggatg cttcgggtag ccactctccg catcgcggcg tagcgtgggt catcgcctcg
      841 ggagaggaga cgacggtcat ggtggcgatg gccgaagtcg tggtgttcgt acttctggcg
      901 gctgtctttg tctggtggtt ccgccggaca ccgatgtacc gggctcatcg ccggactggc
      961 gttgtgccgt ccgggggtca gttctttggg gctgctgacc cgacgtggca cggccagcgg
     1021 cacgcgccgc cgctgcgtcc agaattacga ccggacgatg accccgtgcc cacgcgctcg
     1081 cggcgatggt ggaggtggca cagcaggcag cagggggcga aagacacgat ctgacacgcg
     1141 gcgcgtgccc gccgcccatg acgactgggc tggaacgtgt tcctctgccg ctgacctgga
     1201 cggcatagac ggctgtcgtt ccaatcacaa cgcaccgcga ccttccgttc cactgctggc
     1261 gtggtggtgt tccaccgcac atcgcaatca gaaccgccgt tcatctgttc gtgatcccgc
     1321 actgcctgag taggcgcacg ctcatgcgct ggcatgaacg gtcctgccct gagcgctcgc
     1381 ttgggattcg gcgctgatgg cgccatcatg gcgccatgcc tagtgtgcag atcaaggacg
     1441 tcccggcgga gacgcacgcc gtcttgcgac agcgcgccgc agccgcgcat cagtcgctgc
     1501 aggagtacct ccgcgccaga ctcatcgacg aggccagccg accgacgctc gatgaggtga
     1561 tggaccgcgc cggtgggcgc gctggaggat cagttccgtt gaaggccgcg gcgagcactc
     1621 tgcgggccga gcgtgctcgt cgttgacgcc agtgttctcg caactgcgct tgctgacgac
     1681 gccgacgatg gtgatcgagc acgatcgcgg ttgcgcggtg aggcagtcgc ggcacccgac
     1741 ctcgtcgacc tcgaggtcac atcggtcctg cgaggacgac tcgcgggagg acacatctcc
     1801 cctcgtcggg ccgagcttgc gctcaccgat ctggtcgcgc taccgctgac gcgatcgccg
     1861 tcgctgccgc tcatcccgcg ctgctgggaa ctgcgcgaca acctgaccgt gtacgacgcc
     1921 gcctatgtcg cgcttgccga agcacttacg gtgcctttgc tgaccgccga cgctcgcgta
     1981 gccaaggcga cgggaccgcg ttgtccaatt gaggttctgc gctagcccga atgggctcct
     2041 gctgctcgat gagtagcggc cgtctcgcgt gacgaggtca ggcagtcaag gtgtagaggc
     2101 gaccctcgcc gtacgtgttg aatccaagtc tggtgaggat tggcttggag gtgttgacgc
     2161 gcgcgtgcac aagcgccagt cgcgcgctgt gatcgtgggc gacttcaagt ctcgtgagga
     2221 gggtttggcg gtatccgccg cgtccgcgca tctcggggag gacgcatccg ccgtacagtc
     2281 gagcgacacc atcgacgacc tggcatccgg cggtcgcgaa tgccgtccga tcggcgtagg
     2341 ccacgacccg gaatccgccg tgctcgtccc ggggatctcc caaggaccgg agttgggcgt
     2401 cacggcgctt gttcgaggac ggctcgcctc cccagactgc tgctgcgacg gtttcggcgt
     2461 cgtcgaggct ctgcgcgtcg gtgaccacca cagcgtgcac gtcgtccggt accggcaccg
     2521 cgggaacacc gcccgacatg tcgtaggcca agatgtccgc tgcgtctgtc gcgactgcgc
     2581 cgcgctggat gagtgtcctc tcaagggcgt cggatgtgga cggcgtcacc caccactctg
     2641 tagaggtgcg gcctcgactt cttgcagcct cacagacggc ggccacagtc tggtcagagt
     2701 ctgtgacgtg ctcgggcatt gcgtagaagc ccatacgcgc ccagtccggg tagtcgatca
     2761 ccacaacgtc atggatgtgc aactcctcag caccgggcgg cacccactgc caagcgtgcg
     2821 aggctccgag cacctgctca cgcgtccaca tgggtcgcac catgccacga acggcactca
     2881 cgtgccgccg tccttccgcg cttctgacga tgagcggctc ctcgtgcaac tagcggttcg
     2941 atcgtgttgt ctcctccacg tgaccaaagg attccggcat caacggcgcg catggctcgg
     3001 tgtcggcctc gccggt
//
LOCUS       DATBFZ010000125         1669 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_2049312, whole genome shotgun sequence.
ACCESSION   DATBFZ010000125 DATBFZ010000000
VERSION     DATBFZ010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 1669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1669
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2049312"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1551)
                     /gene="istA"
                     /locus_tag="VH419_03765"
     CDS             complement(<1..1551)
                     /gene="istA"
                     /locus_tag="VH419_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020578920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HEY7042766.1"
                     /translation="MAQPRSRVDLYAAIRRDARSGMSNRALQRKHGVGFRTVTAALES
                     AWPKERKQPPKRGSRLDAYRVVIDDWLRSDLDAPRKQRHTAKRIFDRLLDEHDGAGVV
                     SYWMVREYVATRRREIRVEVGREPANAFIPQEHLPGREAEVDFGDVAIHLRGELVTCA
                     LFSLRLSYSGKAVHRVSASAGQEAFFEGHVHAFNVLGGVPTGKIRYDNLKAAVASVIG
                     FSRQRVEADRWTAVRSHYGIEAFYCQPGIQGAHEKGGVEGQIGWFRRNHLVPIPEVDS
                     LAELNAMVDAWDEADDARRIGSRARTVGEHFATEQPLLAPLPTEPFETGRWFTPRVDR
                     YAQVTVRMNKHSVPACMVGRQARVLLNASDLVVFDGRTEIARHERLMTKGSTRVDLDH
                     YLEVLLRKPGALPGAKALEQARASGRFTPVHDAWWAAACKARGDADGTRALIEVLMLH
                     RHLPHDHVVAGLATALRAGALTADAVALEARKYNDTADGGADDTTADHQSLMSGDRAR
                     GEGEIPAVR"
BASE COUNT          238 a          560 c          587 g          284 t
ORIGIN      
        1 tcgcacggca gggatctcgc cttctccgcg tgccctgtcg ccgctcatca gggactggtg
       61 gtccgctgtc gtgtcgtcgg ctccgccatc tgctgtgtcg ttgtacttgc gcgcttccaa
      121 cgcgacagcg tccgcggtga gcgcgcctgc acgcagcgcc gtcgcgagtc cggcgacgac
      181 gtggtcgtgt ggaaggtgcc ggtgcagcat caggacctca atgagggcac gggtgccgtc
      241 ggcgtcaccg cgggccttgc aggccgcggc ccaccaggcg tcgtgaacgg gcgtgaaccg
      301 cccggatgcc ctggcctgct ccagcgcttt cgcaccgggt agcgcgcccg gtttgcggag
      361 gaggacctcg agatagtggt ccaggtcgac ccgggtcgag cccttggtca tgagccgttc
      421 gtggcgggcg atctcggtcc tgccgtcgaa cacgaccagg tcgctggcgt tcaggagcac
      481 ccgggcttga cggccgacca tgcacgcggg cacggagtgc ttgttcatcc ggaccgtgac
      541 ttgggcgtac cggtccacgc gcggggtgaa ccagcggccg gtctcgaacg gctcggtcgg
      601 aaggggcgcg aggagtggct gctcggtggc gaagtgttcc ccgaccgtgc gggcgcggga
      661 cccgatccgc cgggcatcgt cggcttcgtc ccaggcgtcg accatagcgt tgagctcggc
      721 gagggagtcg acctcgggga tggggacgag gtggttgcgt cggaaccagc cgatctgccc
      781 ctcgacgccg cccttctcgt gcgcaccttg gattccgggc tggcaataga aggcttcgat
      841 gccgtagtgg gaacggacgg cggtccacct gtcggcctcg acccgctggc gggagaaccc
      901 gatcacgctc gcgacggcgg ccttgaggtt gtcgtagcgg atcttcccgg tcggcacccc
      961 gccgagcacg ttgaaggcgt ggacgtgacc ctcgaagaag gcttcctgcc cagccgaggc
     1021 actgaccctg tgcacggcct tgcccgagta ggagagccga aggctgaaca gcgcgcaggt
     1081 tacgagctcg ccgcgcaggt ggatggcgac gtcgccgaag tcaacctcgg cctcgcggcc
     1141 cgggaggtgc tcttgcggga tgaacgcgtt ggcaggttcc cgtccgacct cgacgcgaat
     1201 ctcacggcgt cgggtggcga cgtactcgcg gaccatccag tacgacacca ccccggcccc
     1261 gtcatgttcg tcaagcagtc gatcgaagat ccgcttcgcc gtgtgtcgct gcttccgcgg
     1321 cgcgtcgagg tccgagcgca gccagtcgtc gatcaccact cggtacgcgt caagccgtga
     1381 gccgcgtttg ggcggttgct ttctctcctt cggccatgca gattccagcg ccgctgtcac
     1441 ggttcggaag ccgacgccgt gcttgcgctg cagcgcgcgg ttggacatcc cagatcgggc
     1501 atcccgtcgg atcgccgcgt acaggtcgac ccgggaacgc ggctgcgcca tcggtggcac
     1561 tcctgacgtg agcacgccgt gggcacgact ttcggcaccg ggccaccgcc cgggaacggt
     1621 gaccgcctgc gaccacagcc cggaatcact ggccctaccg aggtgcctg
//
LOCUS       DATBFZ010000126         6712 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_410644, whole genome shotgun sequence.
ACCESSION   DATBFZ010000126 DATBFZ010000000
VERSION     DATBFZ010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 6712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6712
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410644"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            551..1873
                     /locus_tag="VH419_03770"
     CDS             551..1873
                     /locus_tag="VH419_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020578395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranyl reductase family protein"
                     /protein_id="HEY7042767.1"
                     /translation="MTSTASRAGSALRTASEHQADVIVVGAGPGGSTTAAYLAQAGHD
                     VLLLEKTAFPREKVCGDGLTPRAVRQLVALGIDLDQPGWIRNQGLRIIGGGYRLELPW
                     PDLTDYPSFGLVRTRMDFDEILARHAQKVGARLHEQTAVTGPVLDDAGRVVGVTARPV
                     DDAGRKSGDELSFRAPVVVAADGNSTRLSLAMGLQKRANRPMGVAVRTYYTSPRHDDD
                     WLESWLELWDGKPGESNQLPGYGWIFGVGDGTANVGLGILNTSAAFQNVDYRELLKRW
                     LDHTPEEWGFRDHNMTAPVRGAALPMGFNRKPHYGNGMLLVGDAGGMVNPFNGEGIAY
                     AMESGEMAADVITQALSRTTPESTERVLQGYVTSLDQRYGGYFTLGRVFVQAIGNPHV
                     MQYLTKHGLHHPALMRIALKLLANLTDARGGDVTDRLVNGLSRLAPTA"
     gene            2035..2394
                     /locus_tag="VH419_03775"
     CDS             2035..2394
                     /locus_tag="VH419_03775"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017948722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEY7042768.1"
                     /translation="MNEYVPILGLMLLAALFAIGSVAASSLIGPKRYNRAKLDSYECG
                     IQPTPQPVGGGKFPVKYYITAMLFIVFDIEIIFLFPWAVYFDSMKVFGLVEMVLFIGT
                     VFIAYAYVWRRGGLEWD"
     gene            2419..2973
                     /locus_tag="VH419_03780"
     CDS             2419..2973
                     /locus_tag="VH419_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006133126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HEY7042769.1"
                     /translation="MGIEEKLPSGVLLSTVEGLAGYFRKASLWPATFGLACCAIEMMT
                     SGGPRYDLARFGMEVFRASPRQADLMIVAGRVSQKMAPVLRQIYDQMPNPKYVLAMGV
                     CASSGGMFNNYAIVQGVDHVVPVDMYLPGCPPRPEMLIDSILKLHDDILHSKLGKHRQ
                     AEIEREEHAALSAAPTSEKRGLLR"
     gene            3150..3803
                     /locus_tag="VH419_03785"
     CDS             3150..3803
                     /locus_tag="VH419_03785"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007079638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEY7042770.1"
                     /translation="MDVIDVRHGMFGAAGTGDTSGYGGLVRPVAVPGATQRPYGGWFD
                     VVADRLEQALEPQGALDAVTKVIVHRGEITFHVAREKLVSVMQTMRDDPSLRFEFCSG
                     VSGVHYPNDEGAELHAVYHLLSMTHNRRIRVEVSCPDSDPHMPSVVAVYPTNDWHERE
                     TYDFFGIIFDGHPALTRIQMPDDWPGHPQRKDYPLGGIPVEYKGATIPPPDRRRAYN"
     gene            3803..5188
                     /locus_tag="VH419_03790"
     CDS             3803..5188
                     /locus_tag="VH419_03790"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012923851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HEY7042771.1"
                     /translation="MTDTANPTNTSTRAGEGFTDDPYAVEQETTAGKVFTVTGQDWDS
                     VLSGLDLDADEHIVVNMGPQHPSTHGVLRLVLELDGETVKECRCGIGYLHTGIEKNME
                     YRNWTQGVTFCTRMDYLSPFYNEAAYCFGVERLLDIEHDIPEKAQVMRVLLMEINRIS
                     SHLVAIATGGMEIGALTVMTIGFRERELCLDLFELITGLRMNHAFIRPGGVAQDMPPG
                     ALEKINEFTGLMRKRLPEYAALCNANPIFKRRLVDVGYLDLEGCLALGASGPVLRATG
                     YDWDIRKRQPYCGYETYDFDVCTWDTADSYGRFRIRLDEMWQSLRIVEQCAERLQGLE
                     GAPTMIGDKKIAWPSQLAVGSDGMGNSLDHIRHIMGESMEALIHHFKLVTEGFRVPTG
                     QAYAVVESPRGELGCHVVSDGGTRPYRAHFRDPSFVNLQSAPAMSEGGMVADVIVAVA
                     SIDPVMGGVDR"
     gene            5185..6078
                     /gene="nuoE"
                     /locus_tag="VH419_03795"
     CDS             5185..6078
                     /gene="nuoE"
                     /locus_tag="VH419_03795"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007079640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HEY7042772.1"
                     /translation="MTTPQANTGASSTTQALLEASGYSDETLDELREIAGRYPQPQSA
                     LLPMLHLVQSVDGCVTPRGIETCAAILDLSPAEVSGVATFYTMYKRRPVGDYHVGVCT
                     NTLCAVMGGDLIFDRLKQHLKVGNDETTADGKITLEHVECNAACDYAPVLMVNWEFFD
                     NQTPDSAVKVVDDLRSGREVRATRGARIGNWRDAERVLAGFPDDLADEGPAAGPASLV
                     GLELARSNNWTAPGATGGQPTPETTTDRVAAAEQADTARAESEAKVEESPAQTRHDRD
                     SGDAQSPLFDDSDSDEEGGDA"
     gene            6078..>6712
                     /locus_tag="VH419_03800"
     CDS             6078..>6712
                     /locus_tag="VH419_03800"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002529861.1"
                     /note="part of NADH-ubiquinone oxidoreductase complex I;
                     shuttles electrons from NADH, via FMN and iron-sulfur
                     (Fe-S) centers, to quinones in the respiratory chain; NuoF
                     is part of the soluble NADH dehydrogenase fragment, which
                     represents the electron input part of NADH dehydrogenase;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit F"
                     /protein_id="HEY7042773.1"
                     /translation="MVDTLTPVLSDNWDADRAWTLASYERHGGYQALHKALGLQQDEI
                     IEMVKDSGLRGRGGAGFPTGMKWGFIPQGDGKPHYLVVNADESEPGTCKDIPLMMASP
                     HTLVEGVIISAYAIRANHAFIYVRGEVLHVVRRLQRAVQEAYLAGHLGTDIHGSGYDL
                     DLVVHAGAGAYICGEETALLDSLEGYRGQPRLKPPFPAVSGLYGGPTVINNV"
BASE COUNT         1169 a         2263 c         2247 g         1033 t
ORIGIN      
        1 tgaccaggca aacccgcctt ggtagcggtg gtcttgcctg gtgtccgtgg tggtttgcga
       61 tgtttaaagc ccaagcggcg tgtctacatc gcaaacctcc gatgggcaga gcgggtttgg
      121 gatgtgaata tcgcaaacac caggccggtc agccgctgat gtccgaaacc cgccagtcac
      181 acaccaggcg aacggcacgg gacgtcggcc ggccagctcg ccggacggca acgcttcccg
      241 ctcttcgctg ccatccccac ctcagccctg gcaagcgaca gccggacgac gtcgtccgcc
      301 cagggagata cctgaggagt ccccgaatct ggcttggtgg ggtggtctgt ctggcctacc
      361 cgggcccgac cggccgaacg ccgtgggttc gaaacgcaga cccgttcagg ggcgccggga
      421 tatcggtggt gcactggtca ccggcacgcc ctacactggc cctcagcggg gctcgtgaag
      481 tttttcactt gttcacccag tgtccgatcc accgatccac cagcacgacc ggcgaaggac
      541 tcaacagcgc atgaccagta cggcatcgcg ggcaggctca gcactgcgca cggcctcaga
      601 gcaccaggcc gacgtgatcg tggtcggtgc aggcccgggc ggatccacca cggcggcgta
      661 cctggcccag gcgggtcacg acgtcctcct gctggagaag accgcgttcc cccgcgaaaa
      721 ggtctgcggt gacgggctga cgccgcgggc tgtccgccag ctcgtcgcgc tcggcatcga
      781 cctcgaccaa cccggctgga tccgcaacca gggcctgcgc atcatcggcg gtgggtatcg
      841 gctcgagctc ccgtggccgg acctcaccga ctacccgagc ttcggtctcg tccggaccag
      901 gatggacttc gacgagatcc tggcgcgcca cgcacagaag gtcggggccc ggttgcacga
      961 gcagacggcg gtcaccgggc cggtgctgga cgacgcggga cgggtcgtcg gcgtcaccgc
     1021 ccggccggtc gacgacgcag gtcgcaagtc gggggacgag ttgtcgttcc gggctccggt
     1081 cgtggtggcc gccgacggga actcgacacg actcagcctg gcgatgggcc tgcagaagcg
     1141 ggccaaccgg ccgatgggtg tcgccgtgcg cacctactac acgagcccgc ggcacgacga
     1201 cgactggctc gagtcctggc tcgagctctg ggacgggaag cccggcgaga gcaaccaact
     1261 gccgggatac ggctggatct tcggcgtcgg cgacggtacc gcgaacgtcg ggctcgggat
     1321 cctgaacacg tcagcggcgt tccagaacgt cgactaccgc gagctgttga agcgttggct
     1381 cgaccacacg cctgaggagt gggggttccg cgaccacaac atgacggccc cggtccgtgg
     1441 tgcggcgctg ccgatggggt tcaaccgcaa gccgcactac ggcaacggca tgctgcttgt
     1501 cggcgatgcc ggaggcatgg tcaacccgtt caacggcgaa ggcatcgcgt acgccatgga
     1561 gtcaggcgag atggcggccg acgtcatcac gcaggcactg agccgtacga cgccagagtc
     1621 caccgagcgc gttctccagg gctatgtcac gtcactcgac cagcggtacg gcggctactt
     1681 cacgctcggc cgcgtcttcg tccaggcgat cggcaacccg catgtgatgc agtacctcac
     1741 caagcacgga ttgcaccatc cggcgctgat gcggatcgca ctgaagctgc tggccaacct
     1801 caccgacgcg cgcggcggcg acgtcacgga ccggctcgtc aacggcctgt ccaggctcgc
     1861 ccccaccgcc tgatgcagca gagggggtac ccctaggatg gcgagacgtg cgaggcagcg
     1921 ggtccgacga catgcgcggt gcggatgcgt cgagccgacg gccggccttg acgtgacacc
     1981 cggagcagac cccacgagag cgcccatcgt gacggtggac gggagggaga cggcatgaac
     2041 gagtacgtgc cgatcctcgg cctcatgctc ttggccgcgt tgttcgcgat cgggtcggtg
     2101 gccgcgagct cgctgatcgg tcccaagcgc tacaaccggg cgaagctcga ctcctacgag
     2161 tgcggtatcc aaccgacacc acaaccggtc ggtggcggca agttcccggt gaagtactac
     2221 atcaccgcga tgctgttcat cgtgttcgac atcgagatca tcttcttgtt cccgtgggct
     2281 gtgtacttcg acagcatgaa ggtcttcggg ctcgtcgaga tggtcctgtt catcggcacc
     2341 gtgttcatcg cctacgccta cgtctggcgt cgcggtggac tcgagtggga ctgaggacgg
     2401 aggggctcag gtaggcacat gggcatcgag gaaaagctgc cgtccggcgt cctgctgagc
     2461 acggtcgagg gactggccgg gtacttccgg aaggcgtcgt tgtggccggc gacgttcggc
     2521 ctcgcctgct gtgcgatcga gatgatgacg tccggcggac cccggtacga cctggcccgg
     2581 ttcggcatgg aggtgtttcg cgcctcgccc cgacaggcag acctgatgat cgttgcgggc
     2641 cgcgtcagcc agaagatggc gcccgtactc cgccagatct acgaccagat gcccaacccg
     2701 aagtacgtgc tcgcgatggg tgtctgcgca agcagcggcg ggatgttcaa caactacgcg
     2761 atcgtgcaag gcgtcgacca tgtggtgccg gtcgacatgt atctgccggg ctgcccgccg
     2821 cgtccggaga tgctgatcga ctcgatcctg aagctgcacg acgacatcct ccactccaag
     2881 ctcggcaagc accgtcaggc cgagatcgag cgcgaggagc atgccgcgct gagtgcggcg
     2941 ccgacgtcgg agaagcgagg tctgctgcgg tgacccaaac cccgaaccat ggttccggtt
     3001 ctgcctcgac ttccggtcca ccggagaagc cggtgggcga gcgttcgctg gaggacaagc
     3061 cggtcgagga gcgctcgatc gccgacaagt cgctccatgg ggacgacctg gtcgtcagcg
     3121 acgaccccgg cccgcttccg caccaggagc tggatgtcat cgacgtccgg cacggcatgt
     3181 tcggcgccgc cggcaccggt gacacgtccg ggtacggcgg gctggtgcga cccgtagccg
     3241 tccccggtgc gacgcagcgc ccgtacggcg gctggttcga cgtggtggcc gaccgcctcg
     3301 agcaggcgct ggagccgcag ggcgctctcg acgcggtgac gaaggtcatc gtgcatcgtg
     3361 gcgagatcac cttccacgtg gcgcgcgaga agctcgtctc ggtgatgcag accatgcgcg
     3421 acgacccgtc gttgcgtttc gagttctgca gcggtgtcag cggcgtgcac tacccgaacg
     3481 acgagggtgc cgagctgcac gccgtctacc acctgctgtc gatgacccac aaccggcgga
     3541 tccgcgtcga ggtgagctgt ccggactccg acccgcacat gccgtcggtc gtggccgtct
     3601 acccgaccaa cgactggcac gagcgggaga cctacgactt cttcggcatc atcttcgacg
     3661 gccatccggc gctgacgagg atccagatgc cggacgactg gcctggccat ccgcagcgca
     3721 aggactaccc gctgggtgga atcccggtcg agtacaaggg tgcgaccatc ccgccgcctg
     3781 accgccggag ggcctacaac tgatgaccga cacagcgaac cccacgaaca ccagcacgcg
     3841 ggccggcgag ggcttcaccg acgatccgta cgccgtcgag caggagacga cggcgggcaa
     3901 ggtcttcacg gtcaccggcc aggactggga ctcggtgctg tccggcctcg acctcgacgc
     3961 cgacgagcac atcgtggtca acatggggcc acagcatccc tccacccacg gagttctgcg
     4021 gctcgtcctc gagctcgacg gtgagaccgt caaggagtgc cggtgcggga tcggctacct
     4081 gcacaccggc atcgagaaga acatggagta ccgcaactgg acgcagggcg tgacgttctg
     4141 tacccggatg gactacctct cgccgttcta caacgaggct gcctactgct tcggtgtcga
     4201 gcggctcctc gacatcgagc acgacatccc ggagaaggcg caggtcatgc gcgtcctcct
     4261 catggagatc aaccgcatct cctcgcacct ggtcgctatc gccaccggcg ggatggagat
     4321 cggcgcgctg accgtcatga ccatcggctt ccgcgagcgg gagctctgcc tcgacctctt
     4381 cgagctcatc accgggctgc ggatgaacca cgcgttcatc cgtccaggcg gtgtggccca
     4441 ggacatgccg ccgggtgccc tggagaagat caacgagttc accgggctga tgcgcaaacg
     4501 gttgccggag tacgccgcct tgtgcaacgc caacccgatc ttcaagcgcc gcctcgtcga
     4561 cgtcggctac ctcgacctcg aaggctgtct cgcgctcggc gcgtccggac cggtactccg
     4621 cgccaccggc tacgactggg acatccgcaa acgccagccg tactgcggct acgagaccta
     4681 cgacttcgac gtctgcacct gggacaccgc agactcctac ggccggttcc ggatcaggct
     4741 ggacgagatg tggcagtcac tgcgcatcgt cgagcagtgc gccgagcggc tgcagggcct
     4801 cgagggggcg ccgacgatga tcggtgacaa gaagatcgcc tggcccagcc agctcgccgt
     4861 cggctccgac ggcatgggca actcgctcga ccacatccgg cacatcatgg gggagtcgat
     4921 ggaggcgctg atccaccact tcaagctggt gaccgaaggc ttccgggtgc cgaccgggca
     4981 ggcgtacgcc gtcgtcgagt caccgcgcgg cgagctcggc tgccacgtgg tctccgacgg
     5041 tggcacccgg ccctaccggg cgcacttccg tgacccgtcc ttcgtcaacc tgcagagcgc
     5101 cccggcgatg tcggaaggtg ggatggtcgc ggacgtcatc gtggcagtgg cgagcatcga
     5161 tcccgtgatg ggcggggtcg accgatgaca accccgcagg ccaacaccgg cgcatcgtcg
     5221 acgacacagg cactgctcga agcctctggc tacagcgacg agaccctcga cgagctccgc
     5281 gagatcgcgg gacgctaccc gcagccgcag tcggcgctgc tgccgatgct ccacctcgtg
     5341 cagagcgtcg acgggtgtgt gaccccgcgg ggcatcgaga catgcgcagc gatcctcgac
     5401 ctgagtccgg ccgaggtgtc gggcgtggcg acgttctaca cgatgtacaa gcgccggccg
     5461 gtcggtgact accacgtggg tgtgtgcacc aacacgctgt gcgcggtcat gggcggcgac
     5521 ctgatcttcg atcggctcaa gcagcacctc aaggtcggta acgacgagac cacggccgac
     5581 ggcaaaatca cgctggagca cgtcgagtgc aacgccgcct gcgactacgc cccggtgctg
     5641 atggtcaact gggagttctt cgacaaccag accccggact ccgccgtcaa ggtggtcgac
     5701 gacctgcgca gcggtcggga ggtccgggcg acccgcggcg cccgtatcgg caactggcgc
     5761 gacgccgagc gagtgctggc cggctttccc gacgatctgg ccgacgaagg tcctgcggcc
     5821 ggccccgcat cactcgtggg gctcgagctc gccaggagca acaactggac ggcccctgga
     5881 gcgaccggcg gacagccgac accggagacc acgaccgacc gagtcgccgc cgccgagcag
     5941 gccgacacgg cgcgcgccga gtccgaggcc aaggtcgagg agtcgccggc gcagacacga
     6001 cacgaccgcg actccggcga cgcccagtcc ccgctgttcg acgactccga ctccgacgag
     6061 gaggggggcg acgcctgatg gtcgacacgc tgactcccgt gctgtcggac aactgggacg
     6121 ccgaccgcgc ttggacgctg gcgtcgtacg agcgccacgg cggctaccag gcactgcaca
     6181 aggcgctcgg tctgcagcag gacgagatca tcgagatggt caaggactcc gggctgcgtg
     6241 gacgtggcgg tgccgggttc ccgaccggta tgaagtgggg attcattccg cagggtgacg
     6301 gcaagccgca ctacctcgtc gtcaacgccg acgagtccga gccggggacg tgcaaggaca
     6361 tcccgttgat gatggcgagc ccacacacgt tggtggaggg cgtcatcatc agcgcctacg
     6421 cgatccgggc caaccacgcg ttcatctacg ttcggggcga ggtgctccac gtcgtacgcc
     6481 ggttgcagcg agcggtgcag gaggcctatc tcgccggaca cctcggcacc gacatccacg
     6541 ggtccggcta cgacctcgac ctggtcgtgc acgccggcgc cggcgcctac atctgcggcg
     6601 aggagacggc gctgctggac tccctcgagg gataccgcgg ccagcccagg ctcaagcccc
     6661 ccttcccggc ggtctcgggc ctgtacggcg gccccaccgt catcaacaac gt
//
LOCUS       DATBFZ010000127         2422 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_1066955, whole genome shotgun sequence.
ACCESSION   DATBFZ010000127 DATBFZ010000000
VERSION     DATBFZ010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2422
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1066955"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..497
                     /locus_tag="VH419_03805"
     CDS             <1..497
                     /locus_tag="VH419_03805"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY7042774.1"
                     /translation="TAQDDETIDVFVRSPHCVSVQTQANVFGWSRQVLAKARRHNLVV
                     LARSPLAMGLLSGKYHENTRPAPGDVRLSTPWWTYFDKASMPDWLRRLGTVRDLLTSD
                     GRTLVQGSLAYLWALDPRLIPLPGVRTAAQAIENTESLTHGALPADIADQITTLLADS
                     PERS"
     gene            complement(667..1731)
                     /locus_tag="VH419_03810"
     CDS             complement(667..1731)
                     /locus_tag="VH419_03810"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY7042775.1"
                     /translation="MGIDQVIAARDRVLAADAAEQPVTELLSDVLDALHGCATFSIAA
                     LLLTDTDSMLPFGGVVEGLDPAQCTPFWDNELLDPDFAKFSSLARSAEPVATLSQLTD
                     GDLARSPRYRKLYEPAGGGDEIRVAFTTGTSCWAVAALIRSAELGPFPAAESRAVLDL
                     VPVAARAIRSAVMRRNRATAAPAPTMLVIDSDASLVSTTPGATRVLAELATHGVDIEV
                     PTVVLAAARRAKNLKNATGISMRARGSSGTWFRLHAAPLGARHVAVVLEPAGPADLVS
                     IVLESYGLTQRESEVVPLLARGLSTKEIATEMYISRHTVNDYIKTIFSKCGVTSRGEL
                     VARLFADHILEHHHAATAHR"
     gene            1864..>2422
                     /locus_tag="VH419_03815"
     CDS             1864..>2422
                     /locus_tag="VH419_03815"
                     /inference="COORDINATES: protein motif:HMM:NF039346.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY7042776.1"
                     /translation="MTYEPAVPTIIRRPERIADETWLIHQVQEALGAPSRVYLNSMVI
                     RGAEPVIVDTGTVANRAQWLDDVFGLVDPADVRYVFISHDDVDHTGNLAQVMEQCPNA
                     TLLASWALVERFSNAFEFPLHRCLWVDDGSHLDLADRRLRFVRPPLWDSPTTRGVFDA
                     STDVYWAVDAFATPCSTAIEASVDEL"
BASE COUNT          391 a          799 c          832 g          400 t
ORIGIN      
        1 gtaccgctca ggacgacgag acgatcgacg tgttcgtccg gtcaccacat tgcgtctccg
       61 tccagaccca ggccaacgtc ttcggctggt cacgtcaggt gctggcgaaa gcccgccgcc
      121 acaacctggt cgttctcgca cggtcaccgc tcgcaatggg cctgctcagc ggcaagtacc
      181 acgaaaacac ccggcctgcg cccggcgacg tgcgcctgag cacgccatgg tggacctatt
      241 tcgacaaggc ctcgatgccc gactggctgc gccggcttgg cacagtccgg gacttgctga
      301 caagcgacgg gcggacactc gtgcagggct cgctcgccta cctgtgggcc ctggacccgc
      361 ggctcatccc tctgcccggc gtccgcaccg ccgcacaggc gatcgaaaac accgaatccc
      421 tgacccacgg agccctaccg gccgacatcg ccgaccagat caccaccctg ctcgcagact
      481 cccccgaacg aagctgagga cacaagtccc accgggcgcg cgagtacggc cgatgcctac
      541 tggcgccagc ttccgccgta aggacggcgg ggacaggacg cgggaaggtc gacgcgtagc
      601 cgaacctcga caccgcgacc acatgcgcga tcttgctgcc agatgggatg ttgtcaacag
      661 cagtgttcag cggtgcgcgg ttgctgcgtg gtgatgttcg aggatgtggt cggcgaacag
      721 tctggcgacg agttcgccgc ggctggtcac gccgcacttg gagaagatcg tcttgatgta
      781 gtcgttgacg gtgtggcgtg agatgtacat ctcggtggcg atctctttgg tcgacagtcc
      841 ccgagcgagg agtggcacca cttcggactc gcgttgggtg aggccgtagg actcgaggac
      901 gatggagacg aggtcggctg gaccggcggg ctcgagaacg acggcgacgt ggcgcgcgcc
      961 gagcggggcc gcatggaggc ggaaccaggt gccggagctg ccgcgggcgc gcatcgagat
     1021 cccggtggcg ttcttcagat tcttggctcg tcgcgccgcg gccaggacga ccgtcggcac
     1081 ctcgatgtcg acgccgtggg tggcgagttc ggccagcacc ctggtcgcgc ccggcgtcgt
     1141 tgagacgagt gatgcgtcgc tgtcgatgac gagcatggtt ggcgccgggg cggcggtggc
     1201 gcggttgcgg cgcatcacgg cggagcgaat cgctcgggcg gcgacgggga cgaggtcgag
     1261 gaccgctcgc gattctgcgg cggggaacgg tcccagttcg gcgctgcgga tcagggcggc
     1321 gacggcccag cacgatgtgc cggtggtgaa ggccacccgg atctcgtcgc cgccgccggc
     1381 gggttcgtac agtttgcggt aacgcggact gcgagcgagg tcgccgtcgg ttagttgcga
     1441 cagcgttgcg acgggttcgg cgctacgcgc caaagaactg aacttggcga agtcggggtc
     1501 gagcagctcg ttgtcccaaa acggcgtgca ctgagcgggg tcgagtccct cgacgacgcc
     1561 accgaagggc agcatcgagt cggtgtcggt cagcagcaac gctgcgatcg agaacgtggc
     1621 gcaaccgtgc agcgcgtcga gcacgtcgct gagcagttcg gtcaccggct gctcggcggc
     1681 gtcggcggcg agcacgcgat cccgcgcagc gatgacctgg tcgattccca ctggctacag
     1741 cgtaggacgg gcgaagggtc tggtcatccc cttattcgtg ggggtcgacc cccgtcgatc
     1801 gatccactca acccgaggcc acgggattca cggcgcgtcc aggcatccgt acgttcgcta
     1861 ctcatgacct acgaacctgc cgtccccacg atcatccgcc gtccggagcg gatcgctgat
     1921 gagacgtggc tgatccatca ggtgcaggag gcactcggtg cgccgtcgcg cgtctacctg
     1981 aactcgatgg tgatccgcgg cgctgagccg gtcatcgtcg acaccggcac cgtcgccaac
     2041 cgtgcgcagt ggctggatga cgtgttcggg ctggtcgacc cggccgatgt gcgctacgtg
     2101 ttcatctccc acgacgacgt cgaccacacc ggcaacctcg cccaggtcat ggagcagtgc
     2161 ccgaacgcga cgctgctcgc atcgtgggcg ctggtcgagc ggttctccaa cgccttcgag
     2221 ttccccctgc accgctgcct ctgggtcgat gacggatcgc acctcgacct cgccgatcgc
     2281 cgcctgcgct tcgtgcggcc accactgtgg gactccccga cgacgcgcgg cgtgttcgat
     2341 gcctcgaccg acgtctactg ggccgtcgac gccttcgcca caccgtgttc gaccgcgatc
     2401 gaggcgtcgg tcgacgagct cg
//
LOCUS       DATBFZ010000128         1700 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_6276655, whole genome shotgun sequence.
ACCESSION   DATBFZ010000128 DATBFZ010000000
VERSION     DATBFZ010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6276655"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1700
                     /locus_tag="VH419_03820"
     CDS             <1..>1700
                     /locus_tag="VH419_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020576182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEY7042777.1"
                     /translation="GSIDWFCSPRFDSPSIFGALLDSENGGRFRIRPRTGTFETKQLY
                     LPDTAILITRFLTETGVGEVVDFMPVSSSSTATESHRLARMVRCVRGQMTFDVDIAPR
                     FDYGRTPHKVFVTEHGGVFVGNGTNLTLHAVREPDEERLGQPKIINDSDVNVSMTLRA
                     GQTRGVLIETGTDGPPREIRVAELQQMFDDTARFWRSWLAQSTYTGRWRETLERSAIT
                     LKLMTYAPTGGLVAAPTASLPEQIGGERNWDYRFTWVRDASFSIYALLGMGFTDEAAA
                     FGRWLRDRVVDSANGDTGPLNVMYRVDGTSDLVEETLDHWTGYRGSSPVRIGNGAADQ
                     LQLDIYGEALDSIYFGDQHGLPIGHRGWTRLCDVLDWLVDHWDQPEEGIWETRGGRKD
                     FTYGRIMSWVAFDRGLRLAAKHGRPGSIARWTQERDAIYAQVMSRGWNEERQAFVQHY
                     DDRVLDSSVLRMSSVGLISPLDPMWTSTLRAMDEELVTDSLVYRYDPSATPDGLRGSE
                     GTFSLCTFTYVDALARSGRLDDARLVFEKMQTYANHLGLFSEEIDVRLLVLLLLSVEF
                     "
BASE COUNT          291 a          552 c          560 g          297 t
ORIGIN      
        1 cgggtcgatc gactggttct gttcgccgcg cttcgattcg ccgagcatct tcggcgcctt
       61 gctggactcg gagaacggcg gtcgattccg gatccgtccg cggaccggca cgttcgagac
      121 caagcagctc tacctgcccg acaccgcaat cctgatcacc cggttcctca ccgaaaccgg
      181 cgtcggcgag gtcgtcgact tcatgccggt gtcgtccagc agtaccgcga cggagagcca
      241 tcggctcgcc cgcatggtcc gctgcgtgcg agggcagatg accttcgacg tcgacatcgc
      301 tccgcggttc gactacggcc gcaccccgca caaggtgttc gtaaccgagc acggtggcgt
      361 cttcgtcggc aacggcacca acctcacgct gcacgccgtc cgggagccag atgaggagcg
      421 gctggggcag ccgaaaatca tcaacgactc cgatgtgaac gtgtcgatga cactgcgcgc
      481 cggccagacg cgcggcgtcc tgatcgagac cggcacagac ggtccgcccc gtgaaatacg
      541 agtggccgaa ctgcagcaga tgttcgatga cacagcgcgg ttctggcgct catggctcgc
      601 gcagtcgacg tacacgggtc ggtggcgcga gaccctcgaa cggtccgcca tcacactcaa
      661 actgatgacc tacgctccga ccggcggcct cgtagccgcg ccgaccgcgt cgctgccgga
      721 gcagatcggc ggcgaacgca actgggacta ccggttcacc tgggtccgcg acgcgtcgtt
      781 ctcgatctac gcgctgctgg ggatgggttt caccgacgag gccgccgcct tcggcaggtg
      841 gctgcgcgac cgcgtggtcg acagcgcgaa tggcgacacc ggtccgttga acgtgatgta
      901 tcgcgtggac gggacgtcgg acctggtcga ggagacactc gaccactgga cggggtaccg
      961 cggctccagc ccggtgcgca tcggcaacgg ggccgcggat cagctccaac tggacatcta
     1021 cggcgaagcg ctggacagca tctacttcgg cgaccaacac ggcctgccca tcggccaccg
     1081 cggctggacc cggctctgcg acgtcctcga ctggctcgtc gaccactggg accagccgga
     1141 ggaagggatc tgggagacgc gcggcggccg gaaggacttc acctacggcc ggatcatgag
     1201 ctgggtggct ttcgatcgcg gacttcggct ggccgccaag cacggacgtc ccggctcgat
     1261 cgcgcggtgg acgcaggagc gcgacgcgat ctacgcccag gtcatgtcac gaggatggaa
     1321 cgaggagcgg caggcgttcg tgcagcacta cgacgaccga gtgctggact cgtcggtgtt
     1381 gcgtatgtcc agtgtcgggc tcatctcacc gctcgatccg atgtggacct cgacgttgcg
     1441 ggcgatggac gaggagttgg tgaccgacag tctcgtctac cgttacgacc cgagcgccac
     1501 cccggacggg ctgcgcggtt cggaaggcac gttctcgctc tgcacgttca cctacgtcga
     1561 tgcccttgcc cgttccggcc ggctcgacga cgcgcgactc gtcttcgaga agatgcagac
     1621 ctacgcgaac cacctcgggc tcttctccga ggagattgat gttaggttgt tagtgttgtt
     1681 gttattgagt gttgagtttt
//
LOCUS       DATBFZ010000129         2960 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_1393140, whole genome shotgun sequence.
ACCESSION   DATBFZ010000129 DATBFZ010000000
VERSION     DATBFZ010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2960
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1393140"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2349
                     /locus_tag="VH419_03825"
     CDS             <1..2349
                     /locus_tag="VH419_03825"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HEY7042778.1"
                     /translation="RDNVWGNLNDPVTNARAAYAISHHGTDMRPWTTTHDSNQGTSAD
                     YRTYLSQVEAVTGVQGDDRGVGGYDSPLPPPLPSSGDPGLVQPSTLTDTLTYDQIDGG
                     MLPGANVDTDLDGLTNAFERVAGTSVREADSDHDSLSDAYELGILHSDPTAADSDHDG
                     LSDSTEASLGTSPTSWDTDHDGMSDLVEVRYGSDPLQADAGDGTTATAGQPLLPTVGS
                     SAGDGGPVSASFHQRPTEGYQRVELTPAAEYQHFTLDSSASSQPIVLNGRTIAMLDAA
                     DQRLGVNLAIVQGSYSTSVSASEGTHAGGGAIDVSVSGMSQEQIGRTVGALRAEGFAA
                     WYRTTDQGFDMDHIHAVAIGDRQMSPQAADQVTAYYDGRDGLAGGLTDSMPHGRPVEF
                     GYDEWLAGQPAPSTKLDSFIDNAVAQRGDQYIFGAEANPTDANPDAFDCSELVQWSAH
                     QAGVEVPDGAMYQYLDLKAKGDLMPVEQALHTRGALLFYFSSEPTASGGRPSEAHVAI
                     SLGNGKTIEAKGTDYGVNEFPAEGRFNYAGVIPGLSGTAIADHAESALSTVPAQPMTA
                     YDQIDVGDPVAPTDSDHDGLTDAFEKLAGTDPHAADSDHDSLSDAQEAIGTHTDPLSR
                     DTDHDGRSDRFEIFHHADAGSLPGVAGVVGQGIFAENIRHGTHDDDHDSISNVVEGRL
                     HLDPHSADSDHDGLSDSTELSLGTNPLDADTDHDGFSDAFEVQYGTDPLHAGTGLQEM
                     PMAPGTLGEPVPGTDPLGTDPSDVTGLVDPLADPLGTDPGPQ"
     gene            2421..>2960
                     /locus_tag="VH419_03830"
     CDS             2421..>2960
                     /locus_tag="VH419_03830"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY7042779.1"
                     /translation="MDHVVFQEKLRRPEPLGLSRVRLERQLLDDDGPSVLLVLAPPGS
                     GKTTLLTHVAAAQAAPVAWYRTGPEDVSEAVFVRHVAEALRRAVDKPVDEPDPGVSAA
                     DAESTFGALMGLVDRLGGRPVLLVVDDVHELASTPAEAALERFLRLRPRSLRVLMGSR
                     RPLTLNTPRLLVSGDFVEVD"
BASE COUNT          522 a         1047 c          985 g          406 t
ORIGIN      
        1 cgggacaacg tctggggaaa cctcaacgat cccgtcacga acgcccgggc ggcgtacgcc
       61 atctcccatc acggcaccga catgcgtccc tggacaacga cgcacgactc caaccaggga
      121 acctcggcgg actaccggac ctacctttcg caagtggagg cggtgaccgg cgtccagggc
      181 gacgaccgcg gtgtcggcgg ctatgactca ccgctgccac ctccgctgcc gtcgtcgggc
      241 gatcccgggc tggtccagcc gagcacgctg actgacacgc tcacctacga ccagatcgac
      301 ggcgggatgc tgcccggtgc caacgtcgac accgacctgg acggtctcac caacgcgttc
      361 gaacgtgtcg cggggacgag cgtgcgcgaa gcggacagcg atcacgacag cttgtccgac
      421 gcctacgagc tcgggatcct ccacagcgac ccgaccgcag ccgacagcga ccacgacggc
      481 ctgtcggaca gcaccgaggc gtccctcggc acttcaccga cgagctggga caccgaccac
      541 gacggcatgt ccgacctggt cgaggtccgg tacggttccg atccgttgca ggccgacgcg
      601 ggcgacggta cgacggcgac ggccgggcaa ccgttgctgc cgacggtcgg gagcagtgcc
      661 ggcgacggcg gtccggtgtc cgcgagcttc caccagcgac ccacggaggg gtaccagagg
      721 gtcgagctca ctcccgccgc ggagtaccag cacttcacct tggactcgag cgcgtcgagt
      781 cagccgatcg tcctcaacgg ccgcacgatc gccatgctcg atgccgctga tcaacgtctc
      841 ggcgtcaacc tggccatcgt tcagggctcc tacagcacga gcgtgtccgc ttccgaaggg
      901 actcacgccg ggggaggggc gatcgacgtc agcgtgtccg gcatgtcgca ggagcagatc
      961 ggcaggaccg tcggcgccct gcgtgccgag gggttcgcgg cgtggtaccg gacgaccgac
     1021 caggggttcg acatggacca cattcacgcg gtcgcgatcg gcgaccggca gatgtcacca
     1081 caagccgccg accaggtcac tgcgtactac gacgggcgcg acggtctcgc gggcggtctc
     1141 accgactcca tgccgcacgg caggccggtg gagttcggct acgacgagtg gctcgccggc
     1201 caaccagcgc cttccacgaa actggactcg ttcatcgaca acgccgtcgc acaacgcggt
     1261 gaccagtaca tcttcggcgc cgaggcgaac ccgaccgacg cgaacccgga cgcgttcgac
     1321 tgctccgagc tcgtccagtg gtcggcgcac caagcgggcg tcgaggtacc cgacggagcg
     1381 atgtatcagt acctggacct gaaggcaaag ggcgacctga tgccggtcga gcaggcgctg
     1441 cacacccgcg gcgccctgct gttctacttc tcctcggagc ccactgcgag cggtgggcgt
     1501 cccagcgagg cgcatgtggc gatcagcctc ggtaacggca agaccatcga ggcgaaaggg
     1561 acggactacg gcgtgaacga gttccccgcc gagggccggt tcaactacgc cggggtcatc
     1621 cccggcctgt ccggaacggc gatcgccgac cacgccgaat ccgcgctgtc aaccgtgccg
     1681 gcgcaaccga tgacggcgta cgaccagatc gacgtcggcg accccgtggc gccgaccgac
     1741 agcgaccatg acggtctcac cgacgcgttc gagaagctgg cgggaaccga cccgcacgcc
     1801 gccgacagcg accatgacag cctctcggac gcacaggagg cgatcggcac gcacaccgac
     1861 ccgctcagtc gcgacaccga ccatgacgga cgttccgacc ggttcgagat cttccaccac
     1921 gccgacgcgg gcagcctgcc cggcgtcgcc ggcgtcgtcg ggcagggcat cttcgccgag
     1981 aacatcaggc acggcacgca cgacgatgac cacgacagca tcagcaacgt cgtcgaaggt
     2041 cgactccacc tggacccgca cagcgcggac agcgaccacg atggcctgtc cgacagcacc
     2101 gagctgtcgc tggggaccaa cccgctggac gcggataccg atcacgacgg attcagtgac
     2161 gcgttcgagg tccagtacgg caccgacccg ttgcacgcgg gcaccgggct tcaggagatg
     2221 cccatggcgc ccggcacgct gggggagccg gtcccgggga ccgatccgct cgggacggat
     2281 ccgtccgacg tgacggggct ggtggatccg ctggcggatc cgctcggcac cgatcccggt
     2341 ccgcagtagc ccgatggtcc aagggacggg cttgcggctg cggagtagtc tgaagcggtc
     2401 cccacaacag gtgcaagcca gtggatcatg tcgtcttcca ggagaagctg cgtcgacctg
     2461 agccactcgg tctctcccga gtgaggctcg agcgacagct gctggacgac gacggcccaa
     2521 gtgtgctgct ggtgctcgct ccccccggtt ccggcaagac cacactgctg acccatgtcg
     2581 ctgcggcaca ggccgcgccc gtcgcctggt accgaaccgg tccggaagac gtgagcgagg
     2641 cggtgttcgt gcgccacgtc gccgaggcgc tgcggcgggc ggtcgacaag cccgtcgacg
     2701 agccggaccc gggcgtgagc gccgccgacg cagagtcgac attcggggcg ctcatgggtc
     2761 tggtcgaccg cctcggcggc cggccggtcc tgctcgtcgt cgacgacgtg cacgaactcg
     2821 cgtcaacccc cgcagaggcg gcgctggaac ggttccttcg cctgcggccg cggtcactgc
     2881 gcgtcctgat ggggtcgcgt cgaccgctca cactgaacac accccgcctg ctggtctctg
     2941 gtgacttcgt cgaggtggac
//
LOCUS       DATBFZ010000130         2661 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_5949043, whole genome shotgun sequence.
ACCESSION   DATBFZ010000130 DATBFZ010000000
VERSION     DATBFZ010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2661
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5949043"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2620
                     /locus_tag="VH419_03835"
     CDS             <1..2620
                     /locus_tag="VH419_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020390261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase domain-containing
                     protein"
                     /protein_id="HEY7042780.1"
                     /translation="QDRIVRGYLSAIKSTLRTNFFQHDASGHPKPYLSLKLDPKTVPD
                     LPEPRPAFEVFVYSPRVEGVHLRFGAVARGGLRWSDRREDFRTEVLGLVKAQMVKNTV
                     IVPVGAKGGFYCKQLPDPGIDREAWIAEGIACYKTFISGLLDITDNRVGGETVPPADV
                     VRHDGDDSYLVVAADKGTATFSDIANSVAIDYGFWLGDAFASGGSVGYDHKAMGITAR
                     GAWESVRRHFRERGIDCQSEDFTCVGIGDMSGDVFGNGMLLSEHTKLVAAFDHRHIFL
                     DPSPDAAASYAERRRLFELPRSSWADYDSDLISTGGGVYPRTAKAVPISPEVASVLGI
                     DAGTGTMTPAELMHAILLAPVDLLWNGGIGTYVKAVSELNSDAGDKANDAIRVNGGEL
                     RCQAVGEGGNLGFTQRGRIEYALADGRICTDFIDNSAGVDTSDHEVNIKILLDDVMRA
                     GDMTEKQRGALLASMTDEVGELVLQDNYDQNLALSNAQAVATGLMHVHESWIRRLERR
                     GLLDREIEFLPSVKEFQARRAAGHALTAPELAVLLAYTKIVLADDLLQSDLPDDPFLR
                     GELFSYFPTLLRQEHRESMAKHPLRREIIVTQVVNNMVNFAGTTFFHRLSQETSASAE
                     ELARAHFVCREVFAANDLISQINALDNQVDAAVQTDMRLSVRTLIERACRWMVNNRRP
                     PLDSEATVEHFGTEVQKVMQALPEILTGVQAEEYEARHKTLEEAGVPDDLVRTVAVLP
                     PAYAALEIVEIAKHDQIDALEVARVHSVLAERLGLSELMGMIYTLPRDDRWQTMARAS
                     LRDDLYAVHAALTAQVLASTKSDDSAEDRVASWEADDSVVVNRAKSTLAELTSEHEPD
                     LARMSVGLRVVRTMLT"
BASE COUNT          452 a          921 c          880 g          408 t
ORIGIN      
        1 ccaggaccgg atcgtccggg gctacctgtc ggcgatcaag tcgacgctgc gcacgaactt
       61 cttccaacac gacgcatccg gacacccgaa gccgtacctg tcgctgaagc tggacccgaa
      121 gaccgttccg gacctccccg agccgcgccc tgccttcgag gtcttcgtgt actccccacg
      181 ggtggaaggt gtgcacctgc ggttcggtgc agtagcccgc ggaggcctgc gctggtccga
      241 ccgccgggag gacttccgca ccgaggtgct cgggctcgtc aaggcgcaga tggtcaagaa
      301 caccgtcatc gtccccgtgg gcgcgaaggg cgggttctac tgcaagcagc tgcccgaccc
      361 cggcatcgac cgtgaggcgt ggatagcgga ggggatcgcg tgctacaaga cgttcatctc
      421 gggactgctc gacatcacag acaaccgggt gggtggcgag acggttccgc cggctgacgt
      481 cgtgcgccac gacggcgacg acagctacct ggtggtggcg gccgacaagg gcacggcgac
      541 gttctccgac atcgccaact cggtcgcgat cgactacggc ttctggctcg gtgacgcgtt
      601 cgcgtccggc ggctcggtcg gctacgacca caaggcgatg ggcatcacgg cccgcggtgc
      661 gtgggagtcc gtgcgccggc acttccgcga gcgcgggatc gactgccaga gcgaggactt
      721 cacctgtgtc ggcatcggcg acatgagcgg cgacgtgttc gggaacggca tgctgttgag
      781 tgagcacacc aagctggtcg ccgccttcga ccaccgccac atcttcctcg acccgtcccc
      841 ggatgctgcc gcgtcgtacg ccgaacgccg ccgtctcttc gagctcccac gttcgagctg
      901 ggcggactac gacagcgacc tgatctccac gggcggcggg gtctacccgc ggaccgccaa
      961 ggccgtcccc atctcgcccg aggtggcgtc ggtgctgggc atcgacgccg ggacgggcac
     1021 gatgacgccc gccgagctga tgcacgcgat cctgctggcg cctgtcgacc tgctctggaa
     1081 cggtggcatc gggacgtacg tcaaggcggt cagtgagctc aacagcgacg ccggggacaa
     1141 ggcgaacgac gcgatccgcg tcaacggcgg agagctccgt tgccaagcgg tgggtgaggg
     1201 cggcaacctc gggttcaccc agcgcggccg gatcgagtac gcgctcgccg atggtcgtat
     1261 ctgcaccgac ttcatcgaca actccgccgg cgtcgacacg tccgaccacg aggtcaacat
     1321 caagatcctg ctcgacgacg tcatgcgcgc gggcgacatg accgagaagc agcgcggcgc
     1381 gctgctcgcc tcgatgaccg acgaggttgg cgagctcgtc ctccaggaca actacgacca
     1441 gaacctggcg ttgtcgaacg cgcaggcggt cgccaccggc ctcatgcacg tccacgagtc
     1501 ctggatccgg cggctggagc gccgcggcct gctcgaccgc gagatcgagt tcctcccgtc
     1561 ggtgaaggag ttccaggcac gtcgcgccgc cggtcacgcc ctgacggcac cggagctcgc
     1621 cgtcctgctc gcgtacacca agatcgtgct ggccgacgac ctgctgcaaa gtgacctgcc
     1681 cgacgacccg ttcctgcgcg gggagctctt cagctacttc ccgacactgt tgcgccagga
     1741 gcaccgcgag tcgatggcga agcacccgct gcggcgcgag atcatcgtca cccaggtcgt
     1801 caacaacatg gtgaacttcg ccgggaccac gttcttccac cggctcagcc aggagaccag
     1861 cgccagcgcc gaagagctcg ctcgggctca cttcgtgtgc cgcgaggtgt tcgcggcgaa
     1921 cgacctgatc agccagatca acgccctcga caaccaggtc gacgcagccg tccagaccga
     1981 catgcgattg tcggtgcgca cgctcatcga gcgcgcctgc cgctggatgg tcaacaaccg
     2041 gcgtccgccg ctggactccg aagccacggt cgaacacttc ggtaccgagg tccagaaggt
     2101 catgcaggcg ctgccggaga tcctcaccgg cgtccaggcc gaggagtacg aggcgcgcca
     2161 caagacactc gaagaggccg gtgtgcccga cgacctggtc cgcaccgtcg ccgtactgcc
     2221 tccggcgtat gcggcgctgg agatcgtcga gatcgccaag cacgaccaga tcgatgccct
     2281 cgaggtggcc cgggtccact ccgtgctggc agagcgcctc gggctgtcgg agctgatggg
     2341 catgatctac acgctcccgc gtgacgaccg ctggcagacg atggccaggg cgagcctgcg
     2401 tgacgacctg tacgccgtcc acgccgcgct gaccgctcag gtgctggcgt cgacgaagtc
     2461 cgacgactcg gcagaagacc gggtcgcgtc ctgggaggcc gacgactcgg tggtcgtcaa
     2521 ccgggcgaag tccacgcttg cagagctcac ctcggagcac gagcctgacc tggcccggat
     2581 gtcggtcggc ctgcgcgtcg tccgcaccat gctcacctga cgcactccgg cggtcgagct
     2641 tgtcgagacc ctgggtcgct c
//
LOCUS       DATBFZ010000131         2448 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_1721646, whole genome shotgun sequence.
ACCESSION   DATBFZ010000131 DATBFZ010000000
VERSION     DATBFZ010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1721646"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="VH419_03840"
     CDS             <1..331
                     /locus_tag="VH419_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012923812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY7042781.1"
                     /translation="LIADACAALGDHEAAATETSTARSVLDALGAASALGPITEPVAS
                     SPDGLTERELDVLILLARGKTNRVIAQELFISEKTVASHVGHIFTKLGVSSRSAATAY
                     AYDHHLV"
     gene            complement(453..>2448)
                     /locus_tag="VH419_03845"
     CDS             complement(453..>2448)
                     /locus_tag="VH419_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042782.1"
                     /translation="QTFFSAPPSMPAALSAAVGQQLNGSPLFAFGTSDGSVKLWDPAV
                     TSSTLLSQIAGSTSAAVDALTFTERVDGTVGISDLVAVSSRGNSAHVLRFSGATTLEP
                     RPVAPNGVTSTDVGGIRAWFPGYKSGVLSVINRTDSPVEVDFAFRDNVAFGCFLTQQI
                     GGAPALPADPVTVAPARGADYLLGFLTAGEGGDCASTDFTGQWAIYMTVAPVDDPADR
                     TVAKLVWSRSGQLSVQSVGGSPTLQTEALDLGVERPLGGWEVSTLSPPAPGVPSNINV
                     TGNRLDPAGGTDPPVYRFDVGATAWPLPFSTPPRIQTVLPPMEVRGITSTNADVPLGW
                     LVPEGQPTRATSGTVTLSPESFYWQNPTVGQQITDIYVQVGSTSSSRVNLAGLPAPDP
                     GTPIGSVQACPTSGAGDCTATAAPFATGLDQAKLRVQLFDANSQELPITDPAYSHLYY
                     RDEDGDLITGLIPEDGSSYIRVSPCAGAYPNDGSTTSTIRPPTSCSVGGRFDYLSTTS
                     TDDQQVTAHVGGFFGTVQPAVVEAVAFSPQVQAGSDASQGFYVTPCVDNRGTDACRIA
                     KVGTTTPGLFLTNDPDTQALLIGLQLRSTAQSSLLSLPLEQVSGQPEHIVAEADVNIN
                     NSEISLNTSSGFQPADTVDTWVVSHGAQVPITQIRVGGGN"
BASE COUNT          398 a          781 c          893 g          376 t
ORIGIN      
        1 ccttatcgcc gacgcgtgcg cagcgctcgg cgaccacgag gcggccgcaa cggaaacgag
       61 caccgcccgt tccgtgctcg acgccctcgg tgcggcgtcg gccctgggac cgatcaccga
      121 gcccgtggca tcgtcacccg atggcctgac cgagcgcgaa ctcgatgtgc tcatcctgct
      181 cgcgcgaggc aagaccaacc gagtgatcgc acaggaactg ttcatcagcg agaagaccgt
      241 ggccagccac gtcggccaca tcttcaccaa actcggggtc agctcgcggt ccgccgccac
      301 cgcgtacgcc tatgaccacc atctcgtctg accctgcaaa cgagaaatga cacgcgggcg
      361 acacgcggaa cacgcgaacg acacagggtc cccgcgtccg gcagtccgtg aaggacaaca
      421 gcggtacacc aagaggaacg tctgaccagg cgtcagttgc cgccgccgac cctgatctga
      481 gtgatgggca cctgggcccc gtggctgacg acccacgtgt cgacggtgtc ggccggttgg
      541 aagccggacg aggtattgag gctgatctcg ctgttgttga tgttgacgtc ggcctcggcg
      601 acgatgtgct cgggttggcc gctgacctgt tccagcggca ggctgagcaa cgagctctgc
      661 gcggtgctcc gcagctgcag gccgatgagc agcgcttggg tgtcgggatc gttcgtgagg
      721 aacagtcccg gagtcgtagt gccgaccttc gcgatgcggc acgcgtcggt gccgcggttg
      781 tcgacgcagg gagtgacgta gaagccctgg ctcgcgtcac tgccggcctg cacttggggg
      841 ctgaaggcga ccgcctcgac gacggccggt tggacggtcc cgaagaagcc gccgacgtgg
      901 gccgtgacct gctggtcgtc ggtcgacgtg gtggacaggt aatcgaagcg gccgccgacg
      961 ctgcagctgg tcgggggtcg gatggtcgag gtcgtcgacc cgtcgttggg gtacgcgccc
     1021 gcacacgggc tgacccggat gtagctcgat ccgtcctcgg ggatgaggcc ggtgatcaag
     1081 tcaccgtcct cgtcgcggta gtagaggtgg ctgtaggccg ggtcggtgat cggcagctcc
     1141 tgggagttcg cgtcgaagag ctggactcgc agcttggcct ggtccaaacc ggtggcgaac
     1201 ggcgcggcgg tcgcggtgca gtcgccggcg ccgctcgtcg gacacgcctg cacggaaccg
     1261 atcggggttc ccgggtcagg cgcggggaga ccggcgaggt tcacgcggct cgagctggtc
     1321 gacccgacct ggacgtagat gtcggtgatc tgctgtccga cggtcgggtt ctgccagtag
     1381 aagctttccg gcgacagcgt gaccgtgccc gaagtggcgc gcgtcggttg accctccggc
     1441 acgagccagc cgagggggac gtcggcgttg gtggaggtga tgccccgcac ctccatgggc
     1501 ggcagaaccg tttggatgcg cggcggcgtg ctgaacggca gcggccaggc ggtggcgccg
     1561 acgtcgaagc ggtacaccgg cgggtcggtg ccgccggcgg gatcgagccg attgcccgtc
     1621 acattgatgt tcgacggcac tcctggcgcc gggggtgaca gggtggagac ctcccagccg
     1681 ccgagcggcc gttcgacgcc gaggtcgagg gcttcggtct gcagcgtggg cgagccgccg
     1741 acggactgca ccgacagctg gccgctgcgc gaccagacga gcttggcgac cgtgcggtcg
     1801 gccgggtcgt cgaccggcgc gaccgtcatg tagatggccc attgcccggt gaagtcggtc
     1861 gacgcgcagt ctccaccttc accggcggtg aggaagccga ggaggtaatc ggcgcctcgc
     1921 gccggggcga ccgtgaccgg atccgcgggc agcgccggcg cgcctccgat ttgctgggtg
     1981 aggaagcaac cgaaggcgac gttgtcccgg aacgcgaagt cgacctcgac cggggagtcg
     2041 gttctgttga tgacgctcaa gacaccgctc ttgtagccgg ggaaccaggc gcggatccca
     2101 ccgacgtcgg tcgaggtgac gccgttggga gccaccggac gcggctcgag ggtcgtcgcg
     2161 ccgctgaaac ggagcacgtg ggccgagttg ccccggctgc tcaccgcgac gagatccgag
     2221 atgccgacgg tgccgtcgac gcgctcggtg aacgtcagcg cgtccaccgc ggccgacgtc
     2281 gatccggcga tctgcgacaa caaggtgctc gaggtcaccg ccggatccca cagcttcacg
     2341 cttccgtcgc tcgttccgaa cgcgaacaac ggggaaccat tgagctgctg acctacagca
     2401 gcagacagcg cggcaggcat ggacgggggt gccgagaaga aggtctgc
//
LOCUS       DATBFZ010000132         5377 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_3036664, whole genome shotgun sequence.
ACCESSION   DATBFZ010000132 DATBFZ010000000
VERSION     DATBFZ010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 5377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5377
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3036664"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(31..1248)
                     /locus_tag="VH419_03850"
     CDS             complement(31..1248)
                     /locus_tag="VH419_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008357043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEY7042783.1"
                     /translation="MVLLIVLAVLLVLAAIVLFKSVRIVPQARAGIVERFGKYRTTLS
                     AGLNIVTPFVDRVRYLIDLREQVVSFPPQPVITEDNLVVSIDTVLYFQVTNPVAATYE
                     IANYIQAIEQLTMTTLRNITGGMDLEQALTSRDDINASLRGVLDEATGRWGIRVNRVE
                     LKGIDPPPSIKDSMEKQMRADRDKRAAILTAEGMRQSAILTAEGQKQSAILTAEGDRE
                     ALILRAEGDRQSRILRAQGEGQAILTVFQAIHDGSPDQALLAYQYLQMLPKIAEGDAN
                     KVWIVPSEIGKALEGLGSSIGSIPGIPRDSGGPRQRVDYGEPVSDEPTTGRDSALSSA
                     EQQVAEAIASAEEASRPTHEGGSGPASRGSSATPGAPGDVPQPPTGQPPTGGPGDQPP
                     PPPPPPPPPPPGP"
     gene            complement(1354..1800)
                     /locus_tag="VH419_03855"
     CDS             complement(1354..1800)
                     /locus_tag="VH419_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEY7042784.1"
                     /translation="MDWVQNHAWEAWLIAGLLLAAAEMATMDLTLLMMGFGALAASVA
                     AALGFGVVVQVLVAVIVGVGLLAFVRPPIVRRLHRGPTLQPGPQALIGQSGLVLERVT
                     SQTGRVKLRGEVWSARTFDDSVVVEPGYKVGVAEIDGATAVVYPIQ"
     gene            complement(1855..2661)
                     /locus_tag="VH419_03860"
     CDS             complement(1855..2661)
                     /locus_tag="VH419_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017935287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY7042785.1"
                     /translation="MAAVIELADVSVVRGTSRLLSNVSWRVEADERWVLIGPNGAGKT
                     TLLQVVSTHLYPTEGVVGVLDEVVGTVDVFELRPRIGLTSAALADRIPRDERVSDVVV
                     SASYAVLGRWREAYDDLDHERAAQLLAEVGVTHLADRTFGTLSEGERKRVQIARALMT
                     DPELLLLDEPAAGLDLGGREDLVSTLSMLAMDPLSPATVLVSHHVEEIPPGFTHVLLL
                     RRGEVVAAGPLEETLTEQSLAATFDMPLALERVDGRFSARRQTASHRRSR"
     gene            2750..3484
                     /locus_tag="VH419_03865"
     CDS             2750..3484
                     /locus_tag="VH419_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042786.1"
                     /translation="MFAVPYAARVACWLNAWLSGRESADAVITGVMGEQAHMEFVVFG
                     HDRPGLSPALLLGELRRHGVHQVSAALPAPGDPLGLGGPPRFNADALEAGEALLLHDV
                     DVGLVPRTTGRSTRWESGTAAPPGYLPDVAGADRVLRGALTEAANALADLDVASWNPD
                     VADALLNLRQPARLDVPMSFPSGNAAHTAISGLRCRHIIELAFSDHDGGAVSAWEAEQ
                     RRAALMPLHRASRAAIVAACSSLDGR"
     gene            3492..4736
                     /gene="serB"
                     /locus_tag="VH419_03870"
     CDS             3492..4736
                     /gene="serB"
                     /locus_tag="VH419_03870"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020386281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HEY7042787.1"
                     /translation="MTDESRHDRDEADLAATLLITLVGRDRPGVTAAMFETLAASRVE
                     VLDIEQIVLRGRLVLGILVTAPRDPRKLSAAVEHTAAALDMETEIATGEGDNRPRRGG
                     RAHVTVLGRPLLPAAVAAITGRIADTGANIDRIVRMARYPVTAIEFDVSGTQIGRLRE
                     VLATEAARQSVDVAVQSIGLLRHGRRLVVMDVDSTLVQGEIVDMLAEKAGVADEVSAV
                     TEQAMQGELDFEESLRRRVALLEGLPVAAVDEVYDVMVLAPGARTLVRTLKRLGYRFG
                     IVSGGFSRLTDRLAEDLDIDYTRANELEVVDGRITGRLVGQVVDRAAKAQALMDFAAD
                     SKVTLDRTVAIGDGANDIDMLSVAGLGIAFNAKPVVQEQAHAAVNVPYLDAILYLLGI
                     SREDIEAADAEHGITTPHPAVP"
     gene            complement(4879..>5377)
                     /locus_tag="VH419_03875"
     CDS             complement(4879..>5377)
                     /locus_tag="VH419_03875"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY7042788.1"
                     /translation="LLVMRHSKTEPSAATDHVRRLTERGRRDARSAGAWVAASAYRPD
                     LILSSTAARAEETAEVVAALADVRRVELVDALYGADAYAVLEIVAEQVSDDVGTVLVV
                     GHNPTMAQLGHLLQSEGADEVRFPTSGIGVFGLDVASWADVGTLLDEEGHGRVLASYT
                     PKGYG"
BASE COUNT          821 a         1901 c         1841 g          814 t
ORIGIN      
        1 cgacgaacag tgccagccct tcgagcaggg tcacggccca ggtggtggcg ggggtggcgg
       61 gggtggcggg ggtggcggct ggtcacccgg tccgccggtg ggcggctggc cggtgggcgg
      121 ctgcggtacg tcgccggggg caccaggtgt cgcgctcgac ccgcgggaag ccggcccgga
      181 gccgccttcg tgtgtcggtc gggaggcctc ctcagcagac gcgatcgcct cggccacctg
      241 ctgctcggcc gacgagagcg ccgagtcccg gcccgtggtc ggttcgtccg agaccggctc
      301 accgtagtcg actcgctgtc tcggaccccc gctgtcgcgc gggatcccgg gaatcgagcc
      361 gatcgagctg ccgaggccct cgagcgcctt cccgatctca ctggggacga tccagacctt
      421 gttggcatcg ccctcggcga tcttcggcag catctgcagg tactggtagg ccagcagcgc
      481 ctggtcgggc gaaccgtcgt ggatcgcctg gaacacggtg aggatggcct gcccctcacc
      541 ctgcgcccgc aggatccggg actgccggtc accctccgcg cgcaggatca aggcctcccg
      601 gtcaccctcg gcggtgagga tcgccgactg cttctggcct tcggcggtga ggatcgccga
      661 ctgccgcatg ccctcggcgg tgaggatcgc cgcgcgcttg tcccggtcgg cgcgcatctg
      721 cttctccatc gagtccttga tcgacggagg cgggtcgatg cccttcagct cgacccggtt
      781 cacccggatc ccccagcggc ccgtcgcctc gtcgagcacc ccgcgcagcg aggcgttgat
      841 gtcgtcgcgg ctcgtcagcg cctgctcgag gtccatgcca ccggtgatgt tgcgcagcgt
      901 cgtcatcgtc agctgttcga tggcctggat gtagttcgcg atctcgtatg tcgccgcgac
      961 cggattcgtc acctggaagt agagaacggt gtcgatggac acgacgaggt tgtcctcggt
     1021 gatgacgggt tggggcggga acgacaccac ctgctcgcga aggtcgatga ggtagcgcac
     1081 ccgatcgacg aacggcgtga cgatgttcag gccggccgac aaggtcgtgc ggtacttgcc
     1141 gaagcgctcg acgatgccgg ctcgcgcctg cggcacgatg cgcacggact tgaacagcac
     1201 gatcgcggca aggaccagca ggaccgccag cacgatgagc agaaccatga gcctgaaccc
     1261 cttcgaggac ggtctcgagt cgtatgccgg atgacaccct acccacgcca gggtcgcgcg
     1321 cagtcggcca tcacgccgcc gcgcgcacgg tcgtcactgg atcgggtaga ccacagccgt
     1381 ggcaccgtcg atctcggcca ctcccacctt gtagccgggc tcgacgacga cgctgtcgtc
     1441 gaacgtccgc gccgaccaca cctcaccgcg gagcttgacc cgcccggtct gtgaggtgac
     1501 ccgctccagc acgagtccgc tctgcccgat cagcgcttgc gggccgggct gcagggtcgg
     1561 cccacggtgc aaccggcgta cgatcggcgg gcgcacgaac gcgagcaggc cgacgccgac
     1621 gatcactgcg acgagcacct gcacgacgac gccgaacccg agtgcggctg ccaccgaggc
     1681 tgcaagcgcg ccgaagccca tcatgaggag cgtcaagtcc atggtggcca tctcggcagc
     1741 cgccagcagc agaccggcga tcaaccacgc ctcccaggcg tggttctgca cccaatccat
     1801 gcttcgagcc tacctggcga acctcgcaaa ccggcggctt cgcggaccgc cggctcagcg
     1861 actccggcga tgtgacgctg tctggcgtcg cgcgctgaat cggccgtcga cgcgttccag
     1921 cgccaacggc atgtcgaagg tcgctgcgag cgactgctcg gtcagcgtct cctccaacgg
     1981 gccggcagcg acgacctcac cacgccgaag caacaacacg tgggtgaacc ctggcgggat
     2041 ctcctcgacg tggtggctca ccaagacggt ggccggcgac aacgggtcca tcgcgagcat
     2101 cgacaacgtg ctcaccaggt cctcccggcc gcccaggtcg aggccggccg cgggctcgtc
     2161 gagcagcagc agctccggat cggtcatcag cgcccgcgcg atctgcacgc gcttgcgctc
     2221 cccctcgctc agcgtgccga acgtccggtc cgccaggtgc gtgacgccga cctcggccaa
     2281 caactgggcg gcccgctcgt ggtcgaggtc gtcgtacgcc tctcgccatc ggccgaggac
     2341 ggcgtacgac gccgacacga cgacatcact gacccgctcg tcccgcggga tccggtcggc
     2401 cagagccgca ctggtgaggc cgatacgagg tcgtagctcg aagacgtcca cggtcccgac
     2461 cacctcgtcg aggacgccca cgacgccctc ggtcggatac agatgcgtgc tcaccacctg
     2521 gagcagggtc gtcttgccgg caccgttcgg cccgatcagc acccaacgtt cgtccgcctc
     2581 gacccgccac gacacgttgc tgaggagccg gctggtgccg cggacgacgg acacgtcagc
     2641 cagttcgatg acggctgcca tcgcgctcct tgccgtggga tccggtgcgg gctcgtgcgt
     2701 gtccaaccta ttgcacccat cgatgcgaaa cggctcgtag gctcgtcccg tgttcgccgt
     2761 accgtacgcc gctcgggtgg cctgctggct gaacgcctgg ctgtccggcc gggagagcgc
     2821 cgacgccgtg atcaccggcg tcatgggcga gcaggcgcac atggagttcg tcgtcttcgg
     2881 tcacgaccgg ccgggactgt cacccgcgct gctgctgggt gagctgcgac gtcacggcgt
     2941 acaccaggtg agtgcagcgc tgccggctcc gggcgaccca ctcggactgg gcggacctcc
     3001 gaggttcaac gccgacgcgc tcgaagccgg ggaggcgctg ctgcttcacg acgtcgacgt
     3061 cggtctggtc ccccgtacaa ccggtcggtc gacacgatgg gagtccggca cggcagcgcc
     3121 gccgggctac ctgcccgacg tcgccggcgc ggaccgcgtg ctgcgtggag cactgaccga
     3181 ggcggcgaac gctttggcgg acctcgacgt cgcctcgtgg aaccctgacg tcgccgacgc
     3241 gctgctgaac ctgcggcaac cggcccgtct cgatgtcccc atgagctttc cgtccggaaa
     3301 cgcggcgcac acggcgatca gcggcttgcg gtgccggcac atcatcgagc tggcgttctc
     3361 cgaccacgac ggcggcgcgg tgtcggcgtg ggaggcggag cagcggcgcg ccgcgctcat
     3421 gccgctgcac cgggcatcgc gggccgccat cgtcgctgcg tgctcttccc tggacggacg
     3481 ctgagccgag catgaccgac gagagccggc acgaccgtga cgaggccgac ctggccgcga
     3541 cactcctgat cacgttggtg ggtagggacc gtcccggcgt gacagccgcg atgttcgaga
     3601 cacttgccgc gtcgcgcgtc gaggtgctcg acatcgagca gatcgtgctc cgggggcggc
     3661 tggtcctcgg gattctcgtg acagccccac gcgacccgcg caagctgagc gcggccgtcg
     3721 agcacaccgc ggcagccctc gacatggaga ccgagatcgc gaccggcgag ggcgacaacc
     3781 gcccccgtcg cgggggtcgt gcacacgtga ccgtactcgg acggcccctg ctgcccgcag
     3841 cggtggcggc catcaccggg cggatcgccg acaccggcgc gaacatcgac cggatcgtgc
     3901 ggatggctcg ataccccgtg acggccatcg agttcgatgt ctccggaacg cagatcggac
     3961 ggttgcgtga ggtgctggcg accgaggccg cccggcagag cgtcgacgtc gcagtccaga
     4021 gcatcgggct gctgcgccac ggccggcgac tcgtcgtgat ggacgtcgac tccacattgg
     4081 tccagggcga gatcgtcgac atgctggccg agaaggccgg ggtggcggac gaggtcagcg
     4141 cggtcaccga gcaggcgatg cagggggagc tggacttcga ggagtcactg cgccggcggg
     4201 tggcgctgct ggagggtctt cctgtcgccg ccgtcgacga ggtgtacgac gtcatggtgc
     4261 tcgcgcccgg tgcccggacc ctcgtccgga cgctcaaacg tctcggctac cggttcggca
     4321 tcgtgagcgg tgggttcagc cggctgaccg accggctcgc agaggacctc gacatcgact
     4381 acacacgggc gaacgagctc gaggtggtcg acggtcggat cacgggacgg ttggtgggcc
     4441 aggtggtcga ccgggccgcg aaggcgcagg cgctcatgga cttcgcggcc gactccaagg
     4501 tcaccctgga tcggaccgtg gcgatcggcg acggagccaa cgacatcgac atgttgtcgg
     4561 tggccggtct cgggatcgcg ttcaacgcca agccggtcgt gcaggagcag gcgcacgccg
     4621 ccgtcaacgt cccctacctc gacgcgatcc tgtacctcct cggcatcagt cgcgaggaca
     4681 tcgaggccgc cgacgccgag cacggcatca cgacccctca ccccgccgtc ccctgaccag
     4741 tggccgtggt cggtttgcga tgtttacagc ccgcccggcc tgtggacatc gcaaaccagc
     4801 gatgcaagga gggagtctgc gatggagacc tgccaggttt ggagcgcaag gcgatcgtca
     4861 cctgactcga gcccggtgtt agccgtagcc cttcggcgtg tacgacgcca gcacccggcc
     4921 gtgcccttcc tcgtcgagca gggtccccac gtccgcccag ctcgccacat cgagcccgaa
     4981 gactccgatc cccgacgtcg ggaagcgcac ctcgtcggcg ccctcgctct ggagcaggtg
     5041 tccgagctgc gccatcgtcg ggttgtggcc gaccaccagc acggtcccca cgtcgtccga
     5101 gacttgctct gccacgatct ccagcactgc gtacgcatcc gcgccgtaca gggcgtcgac
     5161 cagctccacc cggcgtacgt ccgccagcgc cgccacgacc tccgcggtct cctccgcccg
     5221 ggcggctgtc gaggagagga tcaggtccgg acggtacgcc gaggccgcca cccatgctcc
     5281 ggcggacctg gcgtcacgac ggcctcgttc ggtgagccgt cgaacgtggt cggtcgccgc
     5341 ggacggctcg gtcttggagt gccgcatcac cagcagc
//
LOCUS       DATBFZ010000133         4483 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_3037137, whole genome shotgun sequence.
ACCESSION   DATBFZ010000133 DATBFZ010000000
VERSION     DATBFZ010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 4483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4483
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3037137"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(85..1023)
                     /locus_tag="VH419_03880"
     CDS             complement(85..1023)
                     /locus_tag="VH419_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019512983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY7042789.1"
                     /translation="MPKERVERDEEDLVRLYLTDIGQYPLLTKDDEVRLAQAIEGGNQ
                     ARLNLDRAGRDLTPARKRELRRQAREGEDAERTFVQSNLRLVVSIAKKYQASGLPLLD
                     LIQEGNLGLMHAVEKFDWRKGFKFSTYATWWIRQAITRGIANTGRTIRLPVHAGDTLA
                     RLQKARARLELKLGRPATLAELSAEVEMPEDKVTEALRFAAEPLSLSEPLREDGDAEL
                     GDVVEDRSAESPFEVAATALLPEEIARLLAPLDEREREILKLRFGLDRGEPRTLEEVG
                     EHFNLTRERIRQIEARAMSKLRHPSSDTGARDLLTV"
     gene            1314..1628
                     /locus_tag="VH419_03885"
     CDS             1314..1628
                     /locus_tag="VH419_03885"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HEY7042790.1"
                     /translation="MAGDMELTMVTMVFDSSDAEALQSILAKYVVLSRGHEGCRNIDL
                     CASATTPGRFVIIQKWESPQAQQAHFDSQDMVEMASACTGLLSRPPSIDLLETISAHD
                     LH"
     gene            complement(1710..2270)
                     /locus_tag="VH419_03890"
     CDS             complement(1710..2270)
                     /locus_tag="VH419_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042791.1"
                     /translation="MSVTVTPETFSLVKFDAGEIKSVVEELLPAIGFPADVEVHIEVD
                     ESTPLGQAKVTSYDDPIVISVESGALEDPKRPRQVSPTGAADVLGRLLFRIHDRRSDG
                     FGDPPADGDLTMAQSVAWDVYCIGRLVRLGFKHYDNRQRRLYHFRNRHGFTDAADEAF
                     EVLWTSDDLTWSDIAALSDKAAAAAA"
     gene            complement(2267..3103)
                     /locus_tag="VH419_03895"
     CDS             complement(2267..3103)
                     /locus_tag="VH419_03895"
                     /inference="COORDINATES: protein motif:HMM:NF016212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF455 family protein"
                     /protein_id="HEY7042792.1"
                     /translation="MRRIFPADLLARDERFVRLTTEERFNDPRSPGSRSGGRGATRLT
                     PARADAPDAARTLMHGIFMGEVQALEGAGRTCWDFETGDGPDDTPFALKLDMARQCWD
                     EARHTEISCKLMDWMGTELGEFSESTFLYEAACNPDPVLRLTGVNRALEGLAIDVFNT
                     MKEFGDVAGDPVLEFCEDWMLADEVTHVKMGSDWLRRLTETDKERRERALEFQRVVDK
                     LFSLGGFRGEDDDSPIKLARRFRELAGFETDEMDDLAEISRQAKQDAEAQRQRLAAQA
                     SA"
     gene            complement(3100..3762)
                     /locus_tag="VH419_03900"
     CDS             complement(3100..3762)
                     /locus_tag="VH419_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042793.1"
                     /translation="MADSKKARAPFAPWDRRELPGSFSVEESARRVGNYKWIEMKLFE
                     ALGGWVATVPELDVKMRLGTHCYHHAWHADLWHKRLPELREMNQDRLTEPPNDELVAF
                     VDAMTEPEAPELTIEKLVGVYRVLIPHKIAAYTYHLNNTSTITDAPTIRSLKFALQDE
                     FEDWRDGEMLIQSLIETEEEVDRAVVHQSRLEKLMIEAGGITGPGSIGTSYASAAEEV
                     PA"
     gene            complement(3822..4463)
                     /locus_tag="VH419_03905"
     CDS             complement(3822..4463)
                     /locus_tag="VH419_03905"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEY7042794.1"
                     /translation="MEAIEAVLFDFNGVLTTSPFTHMAALGTDSGVEGEVVLELMLGP
                     YDEDTDHAWHRFERGEITAAEYGTDLFERAAAAQIDIDFTQLANLMSRLEVHEIVVDR
                     VRDLRADGYRTGLVTNNVKEASGQWRTLVPVEDLFEVVVDSSEVGMRKPNPAIYLHAL
                     DLLGGVAPERAVFLDDAPGNVAGARRAGLHAILVDAAEPSGALDELDSLLQRS"
BASE COUNT          710 a         1498 c         1482 g          793 t
ORIGIN      
        1 gagacagtca gctccaccaa cgaaagtgtg tcgaagagac tgaacggtca gcgcgttcga
       61 cacgctttcg gcgggaatag agcgctagac ggtcaggagg tcgcgagcgc cggtgtcgct
      121 cgacgggtgg cgcagcttcg acatggcccg cgcctcgatc tggcggatgc gctcacgggt
      181 caggttgaag tgctcaccga cctcttcgag cgtgcggggc tcgccccggt cgagaccgaa
      241 gcggagcttg aggatctccc gctcgcgctc gtcgagcggt gcgagcagcc gagcgatctc
      301 ctcgggcagg agcgcggtcg cggctacttc gaatggcgat tccgccgaac gatcctcgac
      361 gacatcgccg agctcggcgt cgccgtcttc acgaagaggc tcggacagcg acagcggctc
      421 agcagcgaac cgcagggcct cggtgacctt gtcctcgggc atctcgactt cagcggacag
      481 ctccgcgagc gtcgccgggc ggccgagctt cagctcgagg cgggcccgcg ccttctgcag
      541 ccgggcaagc gtgtcgccgg catgaaccgg caggcggatc gtgcgcccgg tgttggcgat
      601 gccacgggtg atcgcctggc ggatccacca cgtggcgtac gtcgagaact tgaagccctt
      661 ccgccagtcg aacttctcga cggcgtgcat gaggccgagg ttgccctcct ggatcaggtc
      721 gagcagcggg agcccggatg cttggtactt cttcgcgatc gagaccacca ggcgcaggtt
      781 cgactgcacg aaggtccgct ccgcgtcttc gccctcacgt gcctgccggc gcagctcgcg
      841 cttgcgggcc ggagtcaggt cccggccggc gcggtcgagg ttgaggcggg cctgattgcc
      901 accttcgatc gcctgggcga gacggacttc gtcatccttg gtgagcaggg ggtactgccc
      961 gatatcagtg aggtacagac ggacgagatc ttcctcgtcg cgctcgacgc gctctttggg
     1021 caacagaggc ctctctgctc gaaatccaac tgtggggtac ggcggcacgc tgcgacgggg
     1081 agtggtcagc ccgccgagct tctcggccgt cgcacggccc gagtttgtgc tccttacgat
     1141 actggcgagg tcaagagtgc caacccgctc cttttgattc agggatttga gttcacgctg
     1201 cgtgttcttc ggacgccgtc tgacgcccct tgacgtccaa cttgtacttt ttggcagcga
     1261 cggtcaagga cttcggagat accggaagcg cccctgctct acggtgcgta gtgatggccg
     1321 gggacatgga actcaccatg gtcaccatgg tgttcgactc gtccgacgcg gaagcgttgc
     1381 agtcgattct cgccaagtac gtcgtgctga gccggggcca cgaggggtgc cggaacatcg
     1441 acctgtgcgc gtcggccacg accccaggtc ggttcgtcat catccagaag tgggagtcgc
     1501 cgcaggcgca acaggcccac ttcgactcgc aggacatggt cgagatggca agtgcgtgca
     1561 ccggtctgct gtcccggccg ccctccatcg acctcctcga gaccatttct gcccacgacc
     1621 tccactgacc ccggccgaac tccgggtcgt cgtggccccc agaggggcca cggcgcacca
     1681 acattggggg aagttggagc ggcgggccgt caggcggctg cggccgctgc tttgtccgag
     1741 agggcagcga tgtcggacca cgtgaggtcg tccgacgtcc agagcacttc gaacgcctcg
     1801 tcagccgcgt cggtgaagcc gtggcggttg cggaagtggt acaggcggcg ctgccggttg
     1861 tcgtagtgct tgaacccgag acggacgagg cggccgatgc agtacacgtc ccacgccacc
     1921 gactgggcca tcgtcaggtc cccatccgcc ggcgggtcac cgaacccgtc gctgcggcgg
     1981 tcgtggatgc ggaacagcag ccggccgagc acatccgctg cccccgtcgg agacacctga
     2041 cgtggacgct tggggtcctc gagtgccccg ctctcgacgg agatcacgat cggatcgtca
     2101 tacgacgtga ctttcgcctg ccccagcggc gtcgactcgt ccacctcgat gtggacctcg
     2161 acgtcagcgg gaaagccgat cgcagggagc aactcctcga cgaccgactt gatctctccc
     2221 gcgtcgaact tgacgagcga gaaggtctca ggcgtgaccg tgacgctcat gcggacgctt
     2281 gcgcggcaag ccgttgccgc tgcgcctcgg cgtcctgctt cgcctgacgc gagatctccg
     2341 cgaggtcgtc catctcgtcg gtctcgaacc cggcgagctc acggaagcgg cgggccagct
     2401 tgatcgggct gtcgtcgtcc tcgccgcgga acccgccgag cgagaacagc ttgtcgacca
     2461 cacgctggaa ctcgagcgcg cgctcacggc gctccttgtc cgtctcggtg agccggcgca
     2521 gccagtcgct acccatcttc acgtgggtca cctcgtcggc cagcatccag tcctcgcaga
     2581 actcgagcac cggatcgccg gcgacgtcgc cgaactcctt catcgtgttg aacacgtcga
     2641 tggcgagacc ttcgagggcg cggttgacgc cggtcagtcg cagcaccgga tccgggttgc
     2701 acgcggcttc gtagaggaac gtcgactcgg agaactcgcc gagctcggtg cccatccagt
     2761 ccatgagctt gcaggagatc tccgtgtggc gagcctcgtc ccagcactga cgggccatgt
     2821 cgagcttgag cgcgaacggc gtgtcgtccg gaccgtcgcc ggtttcgaag tcccagcacg
     2881 tgcggcctgc gccttcgagc gcctgcacct cgcccatgaa gatgccgtgc atcaacgtgc
     2941 gggcggcatc gggcgcatcg gcccgcgccg gggtgaggcg ggtcgcaccg cgaccgcccg
     3001 agcgtgagcc cggcgatcgc gggtcgttga accgctcctc ggtggtgagc ctgacgaacc
     3061 gctcgtcccg tgcgagcaag tcagccggaa agattcgtct catgctggaa cctcctccgc
     3121 cgccgatgcg tacgacgttc cgatcgaacc cgggccggtg atgccaccgg cctcgatcat
     3181 cagcttctcg agccgagact ggtggaccac ggcgcggtcc acctcctctt cggtctcgat
     3241 gagggactga atgagcattt ccccgtcgcg ccagtcctcg aactcgtcct gcagggcgaa
     3301 cttgagcgac cggatcgtgg gcgcgtcggt gatcgtgctc gtgttgttca ggtgataggt
     3361 gtacgccgcg atcttgtgcg ggatcaggac ccggtagacg cccaccagct tctcgatggt
     3421 gagctcgggc gcctccggct cggtcatcgc gtccacgaac gcaaccagct cgtcgttcgg
     3481 tggctcggtc aaccggtcct ggttcatctc ccgcagctcc ggcagacgct tgtgccagag
     3541 gtctgcgtgc cacgcgtggt gatagcagtg cgtgccgagg cgcatcttga cgtcgagctc
     3601 cgggacggtg gccacccagc cgcccagtgc ctcgaacagc ttcatctcga tccacttgta
     3661 gttgccgacg cggcgagccg attcctcgac cgagaacgag ccgggcagct cccggcgatc
     3721 ccatggtgcg aaaggcgccc gcgccttctt cgagtcggcc atccgtcccc ctgatcggcg
     3781 tggttacgtc cttgcagact cccgcgaact tgaccccacg gtcaagaacg ctgcaggagg
     3841 gagtcgagct cgtccagcgc ccctgacggc tcggcggcgt cgacgaggat ggcgtggagg
     3901 ccggcccgac gtgcgcctgc cacgttgcct ggtgcgtcgt cgaggaacac ggctcgctcc
     3961 ggggcgacgc ctcccagcag gtcgagggcg tggaggtaga tggccggatt gggcttccgc
     4021 atccccacct cggacgagtc gacgacgacc tcgaaaaggt cctcgaccgg gaccagggtc
     4081 cgccactggc ccgacgcctc tttcacgttg ttggtgacga ggccggtgcg gtagccgtcg
     4141 gcccgcaggt cccgcacccg gtccacgacg atctcgtgga cctcgagccg cgacatcaga
     4201 ttggcgagct gggtgaagtc gatgtcgatc tgcgccgctg cggcccgctc gaacaaatcg
     4261 gttccgtact cggccgccgt gatctctcct cgttcgaagc gatgccaggc atggtcggtg
     4321 tcctcgtcgt acggtccgag catcagctcc agcacgacct caccctcgac accgctgtcc
     4381 gttccgagcg ccgccatgtg ggtgaacggg gacgtcgtca gcacgccgtt gaagtcgaag
     4441 aggaccgcct cgattgcttc cacgcgatgt cagccggcgt act
//
LOCUS       DATBFZ010000134        15334 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_5949965, whole genome shotgun sequence.
ACCESSION   DATBFZ010000134 DATBFZ010000000
VERSION     DATBFZ010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 15334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15334
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5949965"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="VH419_03910"
     CDS             <1..212
                     /locus_tag="VH419_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020658011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY7042795.1"
                     /translation="RAERVAGRLRMGTVWINDYHPYVPQAEWGGYKQSGIGRELGGAG
                     LDEYRETKHVWHNIRPTVQNWFDGT"
     gene            209..1936
                     /gene="betA"
                     /locus_tag="VH419_03915"
     CDS             209..1936
                     /gene="betA"
                     /locus_tag="VH419_03915"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008812 - choline dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HEY7042796.1"
                     /translation="MSSSSRPRDPKHWDFIIVGGGSAGCALANRLSADPANSVLVLEA
                     GRSDIKLDPFIHMPAALPIPIGNRLYDWKYESEPEPYMNGRRIFHARGKVLGGSSSIN
                     GMIFQRGNPLDYQRWGADPGMETWDYLHCLPYFKRMETCLAGADRWRGGDGPLVLERG
                     PATNPLFGAFFEAAQQAGFPLTDDVNGYRQEGFAKFDRNIHRGRRLSAARAYLHPVMK
                     RPNLTVETLALTTGIVFDGKRAVGVDYLRAGRLRRRALGGDVILCGGAINSPQLLQLS
                     GVGNADDLRSLGIDVVHHSPGVGANLQDHLEVYIQYASKLPVSIAPGLRWRSRPKIGF
                     DWLFFRKGLGATNHFEAGGFCRSNDEVDYPNLMFHFLPVAIRYDGSSPTKGHGYQVHV
                     GPMYADTRGSVQITSTDPRKHPSLRFNYLSTPTDRKEWVEAIRTARHILNQPAFAPYN
                     DGELSPGCEVETDEQILAWVAADAETALHPSCTAKMGTDEMAVVDPLTMKVHGVDGLR
                     VVDASVMPYVTNGNIYAPVMMVAEKAADLILANPPLPATDVPYYRYRDTMPLAPPSSP
                     SLEESERRS"
     gene            1933..3030
                     /locus_tag="VH419_03920"
     CDS             1933..3030
                     /locus_tag="VH419_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008361885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HEY7042797.1"
                     /translation="MTNTATESSVAESSSAHPTLSVKELWKVFGPKGKADNLIGTPDA
                     DLPRPEMLAKTGCTAAVKDISFEVAPGEVFVVMGLSGSGKSTLVRCLTRLIEPTAGTV
                     DIDGNDITSASDSKLRELRRNYVSMVFQHFGLLPHRPVLDNVAYGLEIRGMGKTERRA
                     KAGEIVDLVGLAGYEASYPDQLSGGMQQRVGLARALANNPSLLLFDEPFSALDPLIRR
                     DMQDEVIRLQEDLQKTMVFITHDLQEAVKLGDRILIMRDGEVVQIGSPAEIVAHPADD
                     YVRDFVSDIPKSHVLTLRYVMREQRPDDSLDGPVMSPDKIVWQAARAALASEHVVQVV
                     EDGKLIGIVDDEAILRVVVAEEEDTAESVPA"
     gene            3027..5087
                     /locus_tag="VH419_03925"
     CDS             3027..5087
                     /locus_tag="VH419_03925"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY7042798.1"
                     /translation="MSTTATATKIGGFDVGVLASIRRHAQSWALVAVIVLWIVIWSFT
                     QGTDTLQISAITHTDVHDWLQARADAIIAAQDNWFLNILNWIADRINDLITWLQELFT
                     VGKFPRPYPEIGWFGTLAIAMWVTYAVAGGRMVPLVTVSFLLFGALGFWEDSIDTLIV
                     TFVAVFFSIAIGIPLGVWMARSKTVTSIITPILDLMQTMPSFVYLLPFVIFFGIGAAC
                     ATLVTLVYALPPVTRITAYAIKNVSATTLEATSSLGQTKLQRLRNVELPMAKRTIIVG
                     VNQTMMAALSMVTIAAFIDSPGLGQPVIEGLIRGDLGGAFVSGMCIVIMAIMLDRTTT
                     AASVRAELAWRSGKQRKRQRLYILAGGGVLTLIAIYLSHNRLDYNQWPSSWDIGTPIS
                     NKVDDFSDWLTSNVSDLTTTIQDKFTVWLLNPMQDLLAESPWYVTFIAIAAIAAIIGG
                     WRALAFTVVCLAGIYYLDLWNNAMITLTSVLVAAAIVMVLAVVIGVWIGRSRTADKVV
                     RPFLDAGQTMPPFVYLVPILILFGSSRFTAIVAGIVYAAPAAIKLVADGIRGVSPTTI
                     EAAESTGTNRWQMITKVQLPMARGSLLLAANQGLLYVLSMIVIGGMVGAGALGFDVVS
                     GFRQADNIGRGLAAGISIVLIGIMLDRITTYGAANRGLASWIKASVKEPKTTAAPAAA
                     RAPA"
     gene            5165..6151
                     /locus_tag="VH419_03930"
     CDS             5165..6151
                     /locus_tag="VH419_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012917777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY7042799.1"
                     /translation="MKRTRTYAALGALVLSAAAALSGCGGDSIENTDTGAAGNKDCGT
                     LNMAVNNWVGYEADAYVVGQVAEKQLGCTVEYKRLDEQVSWKGFGTGEIDVVIENWGH
                     PELQSKYMSAKGGDGSATDFGLTGNDGIIGWYVPPWMADKYPDITDWKNLNKYSDLFK
                     TSETGDKGTFYDGDPGFVTNDEAIIKNLDLNYEVVYAGSETALIEGFRKAEDNKTPYI
                     GYFYEPQWFLSEVPLVHVDLPKWKQGDPVEGPDVHTDYPPYPLNKVVSTDWSKSGSPA
                     VTLVKNFSWTNDDQNLVAKYIAEDKMDPEDAADKWISDNQDKVDAWLQGTSS"
     gene            complement(6318..7316)
                     /locus_tag="VH419_03935"
     CDS             complement(6318..7316)
                     /locus_tag="VH419_03935"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEY7042800.1"
                     /translation="MSGVTLVLVETDSQEATETSIETLTFARDLARRRDGGSLHAVLV
                     GPLEPAVRATALSQLGDQGVDVVHLAQAEALSSYSAASWASAMCGVVASESADAVLAA
                     GTPRGSEIMAHVATRLDAVMAANVVGVAESEPLVVARQVMGGSVLELMSVSSTPVVLT
                     VAGHAVEPASAEPITEPEVRAFEPELAESDLVGRVVRVEAGESDDTAALTGARVVVGA
                     GRGAGSANGFGDLLELTELLGGALGVSRVVTSLGWRPHHEQVGQTGSRISPDVYIACG
                     ISGAIQHWAGCSSAKTILAINTDPDAPMVTKAHYAVIGDLHEVVPAINEELRRRLA"
     gene            complement(7313..8089)
                     /locus_tag="VH419_03940"
     CDS             complement(7313..8089)
                     /locus_tag="VH419_03940"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042801.1"
                     /translation="MANVLVCIKRVPETSGQILLSDDGRSIDARHVGYTLSAHDECAV
                     ELAIQVAKATDGDATVLSLGSDDAVEQLRDALAVGCTSAVRIDADASTFGPADVAAAI
                     AEVVRSREADGTAYDLILVGNDAADTGDFQVGVRLAYALGRPVLTGVANVEMGSDGRA
                     VARGKGPEGTEVFELPLPAVIAVQEGGVEPRYPTIPGRMKAKRTPIDTIGSAVEPAGS
                     GRVRLELPPAQPSVVEILGDGPAAAPAVVDLLYRIGVVAK"
     gene            complement(8093..10609)
                     /locus_tag="VH419_03945"
     CDS             complement(8093..10609)
                     /locus_tag="VH419_03945"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY7042802.1"
                     /translation="MATVPATARVVVIGAGIVGNSLAYHLARLGWSDIVQIDKGPLPN
                     PGGSTGHASNFIFPVDHSREITDLTLDSMRQYKEMGVFTESGGYEVARTEERMEELRR
                     RMSSARAWGIDAELVTPEQVVEKVPYLDPSTIIGAFWTSSVGVVDSLRAGTIMRERAQ
                     QLGALTVVPTVEVTGLDVEDGRIRRVRTSGGDIESDTVVIACGVWSPRIARMAGAHIP
                     LTPAVHQMISVGPVPVLAEREGEISFPIVRDMDTFCYERQHGADMEVGSYAHRPILHD
                     PDDIPSIEQAKLSPTELPFTADDFDPQMEQAYELMPELFDAEGAEQRYAINGLLSLTP
                     DGSPILGETPEVEGLWSAAAVWVKEGPGVGRAVAEWMTDGLPEIDLANSDIARFYPHQ
                     QTRAHITARTSEAFNKTYGIVHPGEQYESDRNQRLAPMHASQRELGAFFFEAVGWERP
                     FWYESNVNLLEEYADHVMPRDHEWDARWWSPIINAEHLAMRDRAGIVDLSAFAIFDVV
                     GPGALDAVQSIIVAQAAVPIGRVIYTPVLDSRGGFRSDLTVMRLAHDRFRVVTGGAHG
                     MVDRKWFADRMPKDGTASVVDLTSAYTTIGLWGPHARDVLGSLTRDDISHEGFPFATC
                     RMIEADSVALLASRISYVGELGWELHVPIELGARVWRMLHEAGEPHGAVPVGIGVYAT
                     TGRIEKGYRAFGFELDSERTIVEAGMQRPKVKTVDFVGREAYLKQRAEDPKAVLCTLS
                     VDDHTSKSGLKRYMLGGEPILSANGKTLTDEHGHHPYVTTAGSAPSLGKHLLMAYLPP
                     DQATVGNQLTVSYMEELYPVTVASSDSTAVFDPGNARIRG"
     gene            complement(10817..11881)
                     /locus_tag="VH419_03950"
     CDS             complement(10817..11881)
                     /locus_tag="VH419_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013860961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ScyD/ScyE family protein"
                     /protein_id="HEY7042803.1"
                     /translation="MAVPATLALLAPMQASAAKTQPQLDVISTDVFAPFNIDVKPHGI
                     YVADGGTETVSLLRFDGTMIPLVTDATGTSGVAKNRSRLAYTTTVTDPDTFENTDAAL
                     NIRGSRGTVVADTHDYEVANNPDQVNSYGIENATQCQIDAVGPPYTGAIDSHAYSVAA
                     WGNKWLLADAGANDILAIDNAGNISTVAVLPPQPHTVTAEEAEALELPDCMIGATYSF
                     EPVPTDVEVGKNGMLYVTTLPGGPEGPALGARGSVYKVNPWNGHVSLVATGFLGATNL
                     ALGNNGKIYVAELFGDQISVVDCSQVKPYVHLTGAVAVERGLRHGNLIAGTLDPTFQG
                     PGSIVRIRPGDKAKSTVSLL"
     gene            complement(12085..13071)
                     /locus_tag="VH419_03955"
     CDS             complement(12085..13071)
                     /locus_tag="VH419_03955"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015071129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEY7042804.1"
                     /translation="MSTPVKVAVTGAAGQIGYSLLFRIASGSILGPDTSVELRLLEIT
                     PALNALEGVVMELDDCAFPTLAGVETGDDPVTIFDGVSLALLVGARPRTKGMERGDLL
                     EANGGIFKPQGQAINDHAADDVRVLVTGNPANTNALIAMSNAPDIPQERFSALTRLDH
                     NRALAQLAAKAGVSVNDLAKMTIWGNHSATQYPDIFHAEVNGKNAAETVNDQEWVESD
                     FIPTVQKRGAAIIEARGASSAASAASATVDAARDWLHGTPDGDWTSMAIRSDGSYGVP
                     EGLVSSFPVTTKGGDWQVVTGLEIDDFSRARIDASAAELADERDAVTQLGLI"
     gene            13205..14425
                     /locus_tag="VH419_03960"
     CDS             13205..14425
                     /locus_tag="VH419_03960"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017986747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEY7042805.1"
                     /translation="MAKIKVTNPVVELDGDEMTRIIWQFIKDRLIHPYLDVELAYYDL
                     GIEHRDATEDQVTVDAANAIKAHGVGVKCATITPDEARVKEFGLKKMWLSPNGTIRNI
                     LGGVIFREPIVISNIPRLVPGWTKPIIIGRHAHGDQYRATNFTVDGPGELTITFTPAD
                     GSEPVQHVVANYPDGGGVAMGMYNFTQSIEDFARASFSYGLQREYPVYMSTKNTILKA
                     YDGAFKDIFARVFDDEFKADFDAKGLTYEHRLIDDMVAAALKWEGGYVWACKNYDGDV
                     QSDTVAQGFGSLGLMTSVLMTPDGKTVEAEAAHGTVTRHFRQHQQGKPTSTNPIASIF
                     AWTGGLKHRGKLDNTPAVTGFAETLERVCVQTVEDGQMTKDLALLVGPDQKWLTTEEF
                     LGALDDNLQKAMAQ"
     gene            14606..>15334
                     /locus_tag="VH419_03965"
     CDS             14606..>15334
                     /locus_tag="VH419_03965"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007079419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEY7042806.1"
                     /translation="MRFLQGAPAHDLTYADVFMVPSRSSVTSRMDVDLTTPDRIGTTI
                     PIVVANMTAISGRRMAETVARRGGLAVLPQDIPIDVAGSAVGWVKARHKVVETPIQVA
                     SDAVVAEVLSLLGKRAHGAAIVVDGGRPVGIITEHDCQEVDRFSRAADVMTTDMLTLD
                     RDVDLRVAFDVLTNHHLDVAPVLDGDQMVGVLTRKGILRSAIYTPAVDQDGRLMVAAA
                     IGINGDVEGRATALLGHGIDLLVVD"
BASE COUNT         2612 a         5167 c         5049 g         2506 t
ORIGIN      
        1 ggcgtgccga gcgggtcgcg ggtcgactca ggatggggac ggtctggatc aacgactacc
       61 acccctacgt cccgcaggcc gagtggggcg gctacaagca gagcggcatc ggccgtgagc
      121 tcggcggcgc gggcctcgac gagtaccgtg agaccaagca cgtgtggcac aacatccggc
      181 cgaccgtgca gaactggttt gacggcacat gagcagctcc agccgacctc gggatccgaa
      241 gcactgggac ttcatcatcg tcggcggtgg ctcggccggt tgcgccctgg ccaaccgcct
      301 ctccgccgac cccgcgaact ccgtgctcgt cctcgaagcc ggccgcagcg acatcaagct
      361 cgacccgttc atccacatgc ctgccgcgct gccgatcccc atcgggaacc ggctgtacga
      421 ctggaagtac gagtccgagc ccgagccgta catgaacggc cgccggatct ttcacgcccg
      481 cggcaaggtg ctcggcgggt ccagcagcat caacgggatg atcttccagc gcggcaaccc
      541 gctggactac cagcgatggg gagccgaccc gggtatggag acctgggact atctgcactg
      601 cctgccctac ttcaagcgca tggagacctg cctcgccgga gccgacaggt ggcggggtgg
      661 cgacggaccg ctggtgctcg agcgaggccc cgccaccaac ccgctgttcg gtgcgttctt
      721 cgaagccgcc caacaggccg gcttcccgct gaccgacgac gtcaacggct accggcagga
      781 agggttcgcc aagttcgacc gcaacatcca ccgcggccgg cggctctccg ccgcacgtgc
      841 ctacctgcac ccggtgatga agcgccccaa tctgaccgtc gagaccctcg ccctcacgac
      901 cgggatcgtc ttcgacggca agcgcgccgt cggggtcgac tacctgcgag ccggcaggct
      961 gcggcgacgc gcactcggtg gcgacgtgat cctgtgcgga ggcgcgatca actccccgca
     1021 gctgctccag ctctccggcg tcggcaacgc cgacgacctg cgctcgcttg gcatcgatgt
     1081 cgtccatcac tcgcccggcg tcggggccaa cctgcaggac catctcgagg tctacatcca
     1141 gtacgcctcg aagctgccgg tgtcgatcgc gcccggactg cgctggcgca gtcgccccaa
     1201 gatcgggttc gactggttgt tcttccgcaa ggggctcggt gccaccaacc acttcgaggc
     1261 cggcgggttc tgccgcagca acgacgaggt cgactacccg aacctgatgt tccacttcct
     1321 gccggtggcg atccgctacg acggctcgtc cccgaccaag ggccacggct accaggtgca
     1381 cgtggggccg atgtacgcag acacccgcgg gtcggtgcag atcacgtcca ccgatccgcg
     1441 gaagcacccc tccttgcgct tcaactacct ctcgacaccg accgaccgga aggagtgggt
     1501 cgaggcgatc cggacggcgc gccacatcct caaccagccg gccttcgcgc cgtacaacga
     1561 tggtgagctg tcgccgggct gtgaggtcga gactgacgag cagatcctgg cgtgggtggc
     1621 ggccgacgcc gagaccgccc tgcatccgtc atgcacagcg aagatgggca cggacgagat
     1681 ggcggtcgtc gacccgctca ccatgaaggt gcacggcgtc gacggtctgc gtgtcgtcga
     1741 cgcctcggtg atgccgtacg tgaccaacgg caacatctac gcccccgtca tgatggtcgc
     1801 cgagaaggcg gccgacctga tcctcgccaa cccaccgctc ccggcgactg acgttccgta
     1861 ctaccgctat cgcgacacca tgccactggc cccgccgtcg tctccatcgc ttgaagagag
     1921 tgaacgccga tcatgaccaa cacagccaca gaatcctcag tcgcagagag ctcgtcggcg
     1981 cacccaacac tcagcgtcaa ggaactctgg aaggtcttcg gccccaaggg caaggccgac
     2041 aacctcatcg gcacacctga cgcagaccta cctcgccccg agatgctggc caagaccggc
     2101 tgcaccgcag cggtcaagga catctcgttc gaggtcgcgc cgggcgaggt cttcgtggtg
     2161 atgggtttgt ccgggtccgg caagtcgacg ctggtgcggt gcctgacccg gctgatcgag
     2221 ccgactgccg gtacggtcga catcgacgga aacgacatca cctcggcctc ggactccaag
     2281 cttcgcgagc tgcgccggaa ctacgtctcg atggtgttcc agcacttcgg actgctaccg
     2341 caccgcccgg tcctcgacaa cgttgcctat ggtctggaga tccgcggcat gggcaagacc
     2401 gagcgccggg ccaaggccgg tgaaatcgtg gatctggtcg gtctggccgg gtacgaggcg
     2461 tcgtaccccg atcagctctc aggcggcatg cagcagcgcg tcggtctcgc gcgtgcgttg
     2521 gccaacaacc cgtcgctgct gctcttcgac gagcccttct cggcgctcga cccgctgatc
     2581 cgtcgcgaca tgcaggacga ggtcatcagg ctccaggaag acctgcagaa gacgatggtg
     2641 ttcatcaccc acgacctgca ggaagcggtg aagctgggcg atcgcatcct catcatgcgc
     2701 gacggcgagg tggtgcagat cgggtcgccc gcagagatcg tcgcgcatcc ggccgacgac
     2761 tacgtgcgtg acttcgtgag cgacatcccg aagtcgcacg tgctgacctt gcggtacgtg
     2821 atgcgagagc agcgccccga cgactctctc gacggtccgg tgatgtcgcc cgacaagatc
     2881 gtctggcagg cggcgcgtgc ggcgctggcg tccgagcacg tcgtccaagt ggtcgaggac
     2941 ggcaagctca tcggcatcgt cgacgacgag gcgatcctgc gggtcgtcgt cgccgaagag
     3001 gaggacacgg cggagagtgt cccggcatga gcacgactgc caccgcaacc aagatcggcg
     3061 gattcgacgt cggcgtgctg gcgtcgatcc gccgccacgc gcagtcctgg gcactcgtcg
     3121 cagtgatcgt gctctggatc gtgatctggt ccttcacgca gggcaccgac acgcttcaga
     3181 tctctgcgat cacacacacc gacgtgcacg actggctgca ggcacgagcc gacgcgatca
     3241 tcgctgccca ggacaactgg ttcctcaaca tcctcaactg gatcgccgac cgcatcaacg
     3301 acctgatcac ctggttgcag gagctgttca cggtcgggaa gttcccgcgg ccgtaccccg
     3361 agatcggctg gttcggcacg ctcgccatcg ccatgtgggt cacctacgcc gtcgccggcg
     3421 gccggatggt cccgctcgtc accgtgtcgt tcctgctgtt cggggcgctc gggttctggg
     3481 aagactcgat cgacacgctg atcgtgacct tcgtggcggt cttcttctcc atcgcgatcg
     3541 gcataccact cggcgtgtgg atggcacgaa gcaagacggt cacctcgatc atcacgccga
     3601 tcctcgacct gatgcagacg atgccgtcct tcgtctacct gctgcccttc gtcatcttct
     3661 tcggcatcgg cgccgcatgc gcgaccctcg tcacgctggt ctatgcgctg ccgccggtca
     3721 cccggatcac ggcgtacgcg atcaagaacg tctcggcgac gacgctcgag gcgacctcct
     3781 cactgggtca gacgaagctc cagcggctga ggaacgtcga gctgccgatg gccaagcgga
     3841 cgatcatcgt cggcgtcaac cagaccatga tggccgccct ctccatggtc acgatcgcgg
     3901 cgttcatcga cagccccgga ctgggtcagc ccgtcatcga gggcctgatc cgcggcgacc
     3961 tcggcggcgc gttcgtgtcg ggcatgtgca tcgtgatcat ggcgatcatg ctcgaccgca
     4021 cgacgaccgc ggccagcgtc cgggctgagc tggcgtggcg ctcggggaag cagcggaagc
     4081 ggcagcggct gtacatcctc gccggtggcg gtgtactgac actcatcgcg atctacctgt
     4141 cgcacaaccg actcgactac aaccagtggc cgtcgtcgtg ggacatcggt acccccatca
     4201 gcaacaaggt cgacgacttc agcgactggc tgacctccaa cgtctccgac ctgaccacca
     4261 cgatccagga caagttcacg gtctggctcc tcaaccccat gcaggacctt ctcgcggagt
     4321 cgccgtggta cgtcaccttc atcgccatcg cggcgatcgc cgccatcatc ggcgggtggc
     4381 gggcgctcgc gttcaccgtc gtctgtctcg ccggcatcta ctacctcgac ctgtggaaca
     4441 acgccatgat cacgctcacg tcggtgctcg tcgcggccgc catcgtgatg gtcctcgccg
     4501 tcgtcatcgg cgtctggatc ggtcggagca ggacagcgga caaagtggtc agaccgttcc
     4561 tcgacgcggg tcagacgatg ccgccgttcg tgtatctggt gccgatcctg atcctgttcg
     4621 ggtcgagccg gttcaccgcg atcgtggccg gcatcgtgta cgccgctccg gcggcgatca
     4681 agctggtggc ggacgggatc cgcggtgtct cgccgacaac gatcgaggcg gccgagtcga
     4741 ccggcacgaa ccgctggcag atgatcacca aggtgcagct gccgatggcg cgtgggtcgc
     4801 tcttgctggc ggcgaaccag ggactgctct acgtgctgtc gatgatcgtc atcggcggca
     4861 tggtcggggc gggggccctg ggtttcgacg ttgtcagtgg gttccgtcag gcggacaaca
     4921 tcggccgggg gttggccgcc ggcatctcga tcgtgctgat cgggatcatg ctcgaccgga
     4981 tcacgacgta cggtgcggcg aacaggggcc tggccagctg gatcaaggcg tcggtcaagg
     5041 agcccaagac gacggccgcc cctgccgccg cgcgcgcgcc ggcgtaggga tcgacgagtc
     5101 aggcaaccag ggtggacggg gatcgaacaa gcagaccgca agtctggaca ggaagaagga
     5161 gcacatgaag aggacccgga cgtacgcagc actcggtgct ctcgtcttga gtgctgcggc
     5221 agccctgagc ggttgtgggg gcgacagcat cgagaacacc gacaccggcg ccgcagggaa
     5281 caaggactgc gggacgctca acatggccgt caacaactgg gtgggctacg aagccgacgc
     5341 gtacgtcgtc ggacaggtgg ccgagaagca gctcggttgc accgtggagt acaagcggct
     5401 ggacgagcag gtctcgtgga agggcttcgg caccggcgag atcgatgtcg tgatcgagaa
     5461 ctggggccac cccgagctgc agtcgaagta catgtcggcc aagggcggcg acggcagtgc
     5521 gaccgacttc ggtctgaccg gcaacgacgg catcatcggc tggtacgtgc cgccgtggat
     5581 ggccgacaag taccccgaca tcaccgactg gaagaacctg aacaagtact cggacctgtt
     5641 caagaccagc gagaccggcg acaagggcac cttctacgac ggcgacccgg gcttcgtgac
     5701 caacgacgag gcgatcatca agaacctcga tctcaactac gaagtcgtct atgcggggag
     5761 tgagaccgcc ctcatcgaag ggttccgcaa ggcggaggac aacaagaccc cgtacatcgg
     5821 ttacttctac gagccgcagt ggttcctctc cgaggtgccg ctggtgcacg tcgacctgcc
     5881 gaagtggaag cagggtgatc cggtcgaggg acctgacgtg cacaccgact acccgccgta
     5941 cccgctgaac aaggtcgtct ccacggactg gtcgaagtcc ggcagcccgg cggtcaccct
     6001 ggtcaagaac ttcagctgga ccaatgacga ccagaacctg gtcgccaagt acatcgccga
     6061 ggacaagatg gatcccgagg acgcggcgga caagtggatc tccgacaacc aggacaaggt
     6121 cgacgcctgg ctgcaaggca cgagcagctg accccgacct ccgttgaggt ttgcgatgta
     6181 aacagcccgg aaggcgtgtc tacatcgcaa acctggggtg gaaggtgcgg gtctgcgctg
     6241 cagagcttcc gccaggactt gcagaacgct ggcggcctac gccgctaacg ctggtgaaac
     6301 gagatcctgc gcggcggtca ggccagccgg cggcgcagct cctcgttgat cgccggcacg
     6361 acctcgtgca ggtcgccgat gacggcgtag tgcgccttcg tcaccatcgg cgcgtccggg
     6421 tcggtgttga tcgcgaggat cgtcttcgcg ctcgagcaac cggcccagtg ctggatggcg
     6481 ccgctgatac cgcacgcgat gtagacgtcg ggcgagatgc ggctgccggt ctgacccacc
     6541 tgctcgtgat gcggccgcca ccccaggctc gtgaccaccc tggacacgcc gagggcgccg
     6601 ccgagcagct cggtcagctc cagcaggtca ccgaacccgt tggcgctgcc cgccccgcgg
     6661 cctgccccga ccacgacacg ggcaccggtc aacgcggcgg tgtcgtcgct ctcacccgcc
     6721 tccacgcgta cgacgcgccc gaccaggtcg gactcggcca gctcgggctc gaaggcccgg
     6781 acctcgggct cggtgatcgg ctcggcggac gcgggttcga ccgcatggcc tgcaacggtc
     6841 agcaccaccg gcgtactgga caccgacatc agctcgagca ccgacccgcc catgacctga
     6901 cgtgccacga cgagcggctc gctctcggcg acgcccacga cgttggccgc catgaccgcg
     6961 tcgagccggg tcgcgacgtg cgccatgatc tcgctgccgc gcggtgtgcc tgcggccagc
     7021 acagcatcgg ccgactccga cgccacgaca ccgcacatcg cactcgccca gctggcggcg
     7081 gagtacgacg acaacgcctc cgcctgggcg aggtggacga cgtcgacacc ctggtcgccc
     7141 agctgcgaca gggcagtcgc tcggacggca ggttcgagcg gtcccaccag cacggcgtgc
     7201 aacgaaccgc catcccggcg gcgcgccagg tcgcgtgcga acgtcaacgt ctcgatggac
     7261 gtctccgtgg cctcctgcga gtcggtctcg accaggacca gcgtcacacc gctcacttcg
     7321 ccaccacccc gatccggtag agcaggtcga caaccgccgg cgccgccgct gggccgtcac
     7381 cgaggatctc caccacgctc ggttgagccg gcggcagttc cagccgcacc cgcccgctgc
     7441 cggccggttc gacagcggag ccgatggtgt cgatcggcgt gcgcttcgcc ttcatccgac
     7501 ccggaatcgt cggatagcgc ggctcgacgc cgccttcctg caccgcgatg acggctggca
     7561 gcgggagctc gaacacttcg gtgccctccg gccccttgcc ccgtgcgacg gctcgcccgt
     7621 ctgagcccat ctcgacgttc gcgacgccgg tcaggacggg tcggcccagc gcgtaagcca
     7681 gtcggactcc gacctggaag tccccggtgt cggccgcgtc gttgccgacc aggatcaggt
     7741 cgtacgccgt cccgtccgcc tctcgcgacc tcaccacctc cgcaatcgca gcggccacgt
     7801 cggccgggcc gaacgtcgac gcgtcggcgt cgatgcgcac agcggaggtg cagccgaccg
     7861 ccaacgcgtc ccggagctgt tcgacggcgt cgtccgagcc gagcgagagg actgtcgcgt
     7921 ccccgtcggt cgccttcgcc acttggatcg cgagctcgac cgcgcactcg tcgtgcgcgc
     7981 tcagcgtgta cccgacgtgc cgcgcgtcga tcgacctgcc gtcgtcggac agcaggatct
     8041 ggccggacgt ctccgggacc ctcttgatgc agacaagaac gttcgccatg gcctaccccc
     8101 tgatccgcgc gttgccgggg tcgaacacgg cggtcgagtc cgacgacgcc accgtgaccg
     8161 gatacagctc ctccatgtag gagaccgtca gctggtttcc gaccgtggcc tggtccggcg
     8221 gcaggtacgc catcagcaga tgcttgccga gcgaaggggc ggacccggcg gtcgtgacgt
     8281 acgggtggtg accgtgctcg tcggtcaacg tcttgccgtt cgcactcagg atgggctctc
     8341 cgccgagcat gtagcgcttg aggcccgact tcgacgtgtg gtcgtcgacc gacagcgtgc
     8401 agagcaccgc cttgggatct tcggcgcgct gcttgaggta ggcctctcgg ccgacgaagt
     8461 ccacggtctt caccttcggc cgctgcatcc ccgcctcgac gatcgtccgc tccgagtcca
     8521 gctcgaaccc gaaggcacgg tagcccttct cgatccgccc ggtggtggcg tagacaccga
     8581 tgccgacggg cacggcgccg tgcggttcac cagcctcgtg gagcatccgc cacacgcggg
     8641 ccccgagctc gatcggcacg tgcaactccc agccgagctc gccgacgtac gagatccgcg
     8701 acgccagcag cgccacggag tcggcctcga tcatgcggca ggtcgcgaac gggaagccct
     8761 cgtgactgat gtcgtcgcgc gtcaacgagc cgaggacgtc gcgggcgtgc gggccccaga
     8821 ggccgatcgt ggtgtacgcc gacgtgaggt ccacgacgga cgccgttccg tccttcggca
     8881 tccggtcggc gaaccacttg cggtcgacca tgccgtgcgc gccaccggtg acgacacgga
     8941 agcggtcgtg ggcgagccgc atcacggtga ggtccgagcg gaagccgccg cggctgtcga
     9001 gcacgggtgt gtagatcacc cggccgatgg gaacggcggc ctgcgcaacg atgatcgact
     9061 ggaccgcatc gagcgccccc gggccgacga cgtcgaagat ggcgaaggcg gacagatcga
     9121 cgatcccggc ccggtcgcgc atggccaggt gttcggcgtt gatgatcgga gaccaccacc
     9181 gagcgtccca ctcgtgatct cgcggcatga cgtggtcggc gtactcctcg agcaggttca
     9241 cgttggactc gtaccagaac ggccgttccc acccgacggc ctcgaagaag aaggcgccca
     9301 gctctcgctg cgatgcgtgc atcggggcga gtcgctggtt tcggtcgctc tcgtactgct
     9361 cgccggggtg gacgatgccg tacgtcttgt tgaaggcctc gctggtccgc gccgtgatgt
     9421 gcgcgcgggt ctgctggtgt gggtagaagc gcgcgatgtc ggagttcgcc aggtcgatct
     9481 ccgggagacc gtcggtcatc cactccgcga ccgcgcggcc gaccccgggt ccttccttca
     9541 cccacaccgc tgccgccgac cagaggccct cgacctcggg tgtctcgccg aggatgggcg
     9601 agccgtctgg tgtcagcgag agcaggccgt tgatcgcata tcgctgttcg gcgccttcag
     9661 cgtcgaacag ctccggcatc agctcgtacg cctgctccat ctgcgggtcg aagtcatccg
     9721 ccgtgaacgg aagttctgtc ggcgacagct tggcctgctc gatcgacggg atgtcgtccg
     9781 ggtcgtgcag gatcggacgg tgcgcgtacg agccgacctc catgtcggcg ccgtgctgcc
     9841 gttcgtagca gaacgtgtcc atgtcccgga cgatcgggaa cgagatctca ccctcccgct
     9901 ccgccagcac cggtacgggg ccgacgctga tcatctggtg cacggccggt gtcagcggga
     9961 tgtgcgcgcc cgccatcctg gcgatccgcg gactccagac gccacaggcg atgaccaccg
    10021 tgtcgctctc gatgtcgcca ccgctcgtcc gtacccgccg gattcggccg tcctcgacgt
    10081 cgagcccggt cacctcgacg gtcggtacga cggtgagagc cccgagctgt tgggcgcgtt
    10141 cgcgcatgat ggtcccggcc cgcagcgagt cgacgacccc cacggacgac gtccagaagg
    10201 cgccgatgat ggtcgacggg tcgaggtacg gcaccttctc gacgacctgc tctggcgtga
    10261 cgagctcggc gtcgataccc catgcgcgag ccgacgacat ccggcggcgg agctcctcca
    10321 tccgctcctc ggtgcgggcg acctcgtagc ccccggactc ggtgaagaca cccatctcct
    10381 tgtactggcg catggagtcc agcgtcaggt cggtgatctc gcgcgagtgg tcgacgggga
    10441 agatgaagtt ggacgcgtgc ccggtcgaac cacccgggtt gggcagcggg cccttgtcga
    10501 tctggacgat gtcgctccag ccgagccggg ccaagtggta tgcgaggctg ttgccgacga
    10561 tgccggcgcc gatcacgacg acgcgggcgg ttgctgggac tgtggccatg gaacggtctc
    10621 tctgtggcga agccgcggtg ttgcgtgata cgcaacttca ccgcactatg tggaacatct
    10681 tgctccatcg tcaccgccaa acacgggcac tgtcaaggcg tcgagcccgt ccgtgcaagc
    10741 tcgaccggcg aacaggcaga actggaggcc aagccgtgtg cggcttggcc tccagcaaga
    10801 acgatcagaa gtcgcgctac aagagcgaga ctgtgctctt cgccttgtct cctggccgga
    10861 tccggacgat cgaacccggt ccctggaagg tcgggtccag cgtgccggcg atgaggttgc
    10921 cgtggcggag tccacgttcc accgcgaccg cgccggtcag gtgcacgtac ggcttcacct
    10981 gcgagcagtc gaccaccgag atctggtcac cgaacagctc ggcgacgtag atcttcccgt
    11041 tgttaccgag cgccaggttc gtcgcgccca agaagccggt cgccaccagc gagacgtggc
    11101 cgttccacgg gttgaccttg tagacggagc cacgggcgcc gagcgccggc ccctcagggc
    11161 cgccgggcag cgtcgtgaca tagagcatgc cgttcttgcc cacctcgaca tcggtcggca
    11221 ccggctcgaa gctgtacgtc gcgccgatca tgcagtccgg gagctcgagc gcctcggcct
    11281 cctcggcggt gaccgtgtgg ggctgcggcg gcagcaccgc gactgtggag atgttgccgg
    11341 cgttgtcgat ggccaggatg tcgttcgcgc ccgcatcggc cagcagccac ttgttccccc
    11401 aggcggcaac ggagtacgcg tgcgagtcga tggctccggt gtacggcgga ccaacggcgt
    11461 cgatctggca ctgtgtcgcg ttctcgatcc cgtagctgtt cacctggtcg gggttgttgg
    11521 ccacctcata gtcatgggtg tctgcgacga ccgtgccgcg agaaccccga atgttcaacg
    11581 cggcatcagt gttctcgaac gtgtcgggat cggtcacggt ggtcgtgtac gccaacctgc
    11641 tgcggttctt ggccaccccg gaagtcccgg tcgcgtccgt gaccaacggg atcatcgtgc
    11701 catcgaaacg aagcaaggag accgtctcgg tccccccgtc ggcgacgtag atgccatgtg
    11761 gcttgacgtc gatgttgaag ggtgcaaaga catctgtgga gatgacatcc agctgtggct
    11821 gcgtcttcgc ggccgacgcc tgcatgggtg ccagcaaggc gagcgtggca ggcacagcca
    11881 cggctacaag taggtgttta cgcacggatc tcccctctca ggggccggga acccacattc
    11941 ccggcccgta tccaagggtt gtacgtgagg tgtcaacagg gcacgggaac agggcgggag
    12001 cagctggcca cgctgcgtca gtgtcctcgg cgtggcgatg ggacaagccg aagatttcgg
    12061 acagaaaccc tggaaaaaat agtgtcagat caggccgagt tgggtcacgg cgtcgcgctc
    12121 gtcggcgagc tcggcggcgc tcgcgtcgat ccgggcacgg gagaagtcgt cgatctcgag
    12181 accggtgacg acctgccagt cgccgccctt ggtcgtgacc gggaacgacg agaccagtcc
    12241 ttcgggtacg ccgtaggagc cgtcggaacg gatcgccatc gaggtccagt cgccgtccgg
    12301 cgtaccgtgc agccagtcac gcgccgcgtc gacggttgcg ctggcggcgc tcgccgcgct
    12361 ggacgcgccg cgggcctcga tgatcgcggc gccgcgcttc tgaaccgtcg ggatgaagtc
    12421 gctctccacc cattcctggt cgttcaccgt ctcggccgca ttcttgccgt tgacctcggc
    12481 gtggaagatg tccgggtact gcgtcgccga gtggttcccc cagatcgtca tcttcgcaag
    12541 gtcgttgacg ctgacgcctg ctttggccgc gagctgggca agcgcgcggt tgtggtcgag
    12601 ccgggtcagg gcggagaacc gttcctgcgg aatgtcgggt gcgttgctca tcgcgatcag
    12661 tgcgttcgtg ttcgccgggt tgccggtgac gagcacccgg acgtcgtcgg cggcgtggtc
    12721 gttgatcgcc tgcccctgcg gcttgaagat cccgccgttc gcctccagca ggtcacctcg
    12781 ttccatgccc ttggtccgtg gtctcgcccc gacgagcagg gcgaggctga cgccgtcgaa
    12841 gatcgtgact gggtcgtcgc cggtctccac gccggcgagc gtggggaagg cgcagtcgtc
    12901 gagctccatc acgacacctt cgagggcgtt cagcgccggc gtgatctcga gcaaccgcag
    12961 ctccacggac gtgtccggtc cgaggatcga gccgctcgcg atgcggaaca gcaggctgta
    13021 gccgatctga ccggctgcgc cggtgacggc gaccttgacg ggtgtgctca cgggaggctc
    13081 ctgggtatgg aaggacgacc acggcgtacg acgatccgac gctagcaaga cgaccgccgg
    13141 ttggctcgac cgcctgcacc tgagatatct tgacgtcaaa caatcttgaa ggagccgaga
    13201 cagcatggcc aagatcaagg tcaccaaccc cgtcgtcgag ctcgacggtg acgagatgac
    13261 gcggatcatc tggcagttca tcaaggaccg gctgatccat ccctatctcg acgtcgagct
    13321 ggcgtactac gacctcggca tcgagcaccg cgacgccacc gaggaccagg tcaccgtcga
    13381 cgctgcgaac gcgatcaagg cccacggtgt cggcgtcaag tgcgcgacca tcacgccgga
    13441 cgaggcgcgc gtcaaggagt tcgggctgaa gaagatgtgg ctgtcgccca acggcacgat
    13501 ccgcaacatc ctgggtggcg tcatcttccg cgagccgatc gtcatcagca acatcccgcg
    13561 gctcgtccca ggctggacga agccgatcat catcggccgt cacgcgcacg gcgaccagta
    13621 ccgtgccacg aacttcaccg tcgacggccc tggcgagctg acgatcacgt tcacgccggc
    13681 cgacggctcg gagccggtcc agcacgtcgt cgcgaactac cccgatgggg gcggtgtcgc
    13741 gatgggtatg tacaacttca cgcagtcgat cgaggacttc gcgcgggcgt cgttctcgta
    13801 cgggctgcag cgcgagtacc cggtctacat gtcgacgaag aacacgatcc tcaaggcgta
    13861 cgacggcgcg ttcaaggaca tcttcgcccg cgtcttcgac gatgagttca aggccgactt
    13921 cgacgccaaa ggcctcacct acgagcaccg gctcatcgac gacatggtcg cggcggcgct
    13981 gaagtgggag ggcggctacg tctgggcgtg caagaactac gacggtgacg tccagtccga
    14041 cacggtcgcg cagggattcg ggtcgctcgg cctgatgacg tcggtgctca tgacgccgga
    14101 cggcaagacg gtggaggccg aggccgcgca cggcaccgtc acccgtcact tccgtcagca
    14161 tcagcagggc aagccgacct cgacgaaccc gatcgcgtcg atcttcgcgt ggaccggtgg
    14221 cctcaagcac cgcgggaagc tcgacaacac gccagcggtc accggcttcg ccgagacgct
    14281 cgaacgggtc tgcgtgcaga ccgtcgagga cggccagatg accaaggatc tcgccctgtt
    14341 ggtcggtcct gaccagaagt ggctgacgac ggaggagttc ctcggcgccc tcgacgacaa
    14401 cctgcagaag gccatggcgc aataagccct cgaccaacgc cgaggtggca ggtttcgttg
    14461 gattctgacg gcgtgtcgac acgagattgc cacctcggca ccacgtgggt gggctgcatc
    14521 catggcagac cttggccgga tgtccgacaa ccgccaacca ggagtcacca gggctcccgg
    14581 ccgcggaagg gcggatactc gtggggtgag gttcctccaa ggtgccccgg cgcacgacct
    14641 gacgtacgcc gacgtgttca tggtcccgtc gcggtcgtcg gtcacgtccc ggatggacgt
    14701 cgacctcacg acaccggacc ggatcggcac cacgatcccg atcgtcgtcg cgaacatgac
    14761 cgcgatctcg ggtcgacgga tggccgagac cgtcgcgcgc cggggcggac tcgccgtact
    14821 gccgcaagac atcccgatcg acgtggccgg gtcagcggtc ggatgggtga aggcgcgtca
    14881 caaggtcgtg gagacgccga tccaggtcgc ttccgacgcc gtcgtggccg aagtcctttc
    14941 actgttgggc aaacgggccc acggcgcggc catcgtcgtc gacggcggtc gaccggtcgg
    15001 catcatcacc gagcacgact gccaggaggt cgaccggttc agccgcgccg ccgacgtgat
    15061 gaccaccgac atgctgaccc tggacaggga cgtcgacctt cgggtcgcgt tcgacgtgtt
    15121 gaccaaccac catctcgacg tggcaccggt gctcgacggc gaccagatgg tcggcgtact
    15181 cacccgcaag ggaatcctgc gatcggcgat ctacacgcct gcggtggacc aggacggtcg
    15241 gctcatggtc gctgctgcga tcgggatcaa cggtgacgtc gaggggcgcg caacggcgct
    15301 gctcggccac gggatcgatc tgctggtggt ggac
//
LOCUS       DATBFZ010000135        12759 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_4311931, whole genome shotgun sequence.
ACCESSION   DATBFZ010000135 DATBFZ010000000
VERSION     DATBFZ010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 12759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12759
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4311931"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..160)
                     /locus_tag="VH419_03970"
     CDS             complement(<1..160)
                     /locus_tag="VH419_03970"
                     /inference="COORDINATES: protein motif:HMM:NF014448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HEY7042807.1"
                     /translation="MRNAVRVGCVSDPIRLLEISETPLDVQTVYALVADSQAGGVAVF
                     VGTVRDHDH"
     gene            complement(144..374)
                     /locus_tag="VH419_03975"
     CDS             complement(144..374)
                     /locus_tag="VH419_03975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042808.1"
                     /translation="MPPNVLATLDSPKRLHPRHNLLDTPPLISTGSISGLPAVELVET
                     PSLISTGSISGMLDQRPARPAALSIGGGAERR"
     gene            373..1812
                     /locus_tag="VH419_03980"
     CDS             373..1812
                     /locus_tag="VH419_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HEY7042809.1"
                     /translation="MADVPPDRPDEPPTDPPGDEPSREPSVPGNPFAGTPFEQIFGGL
                     AGGDTSGLQAMFGQLQRMFAPHEGAVNWDFTKDLARQVVAQHSDRSPDAGDVSRLADV
                     SRLAEHWLDGVTSLPAASPTAQAWSRAAWVEASMPVWRQLVEPIAESVVKAMGQALPT
                     EAQAMAGPMLGILNQIGGALFSQQVGQAIGGLSQEVVTSTDVGFPVTDDHTPAIVLTN
                     AAEFASGLGVDESDVLLYLMLRECAHQRLFLHAPWLRAHIFATIEDYGRGITIDTSKI
                     EENVRGLDPSNLEAMQDALNGGLFDLDHTPEQQAALTRLETLLALVEGWVDEVVGQAT
                     EKAMPQAQALREAVRRRRATGGPAEATFAALVGLELRPRRLREASALFGALRAAEGPV
                     ARDAVWAHPDALPTAADLDDPLAYAQRTKEADSIDVLSQEFDEALTKFLESSRSDASS
                     EPGSATDEPGATSSDDGDDTDESPGSDTR"
     gene            1839..2399
                     /locus_tag="VH419_03985"
     CDS             1839..2399
                     /locus_tag="VH419_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007077142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEY7042810.1"
                     /translation="MSRPASSRSPLYDDAVSALTRWRPPDAEQARLKAAYLAHLERHT
                     DGLSRDCHPDHITASALVVSHDRRQVLLNLHRKYEIWVQFGGHCEAADRRLHEAALRE
                     AAEESGIDGLRLRDETPAQLSRHEVRCGPLRPSHHLDVRYVAVAPQSAQPEPTAESLA
                     VTWFDPADLPAGVDGPLRELIAWALA"
     gene            2803..3405
                     /locus_tag="VH419_03990"
     CDS             2803..3405
                     /locus_tag="VH419_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017934896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEY7042811.1"
                     /translation="MEKPSSAVEVRRSRRRKRTVSAYRDGETIVVMIPARMTRAEEQE
                     WVATMVDRVTRAERRRRPSDRDLEQRAARLSRKYLDGLAEPTSVRWVTNQQARWGSCT
                     PTDGTIRLSERLQGMPAYVIDYVLLHELTHLLAPGHDDDFWSWLQRYDLTERARGFLE
                     GVSNAIHLLPATGTAPDVDPVGDLGDLDDLDDLDTAAAER"
     gene            3664..3822
                     /locus_tag="VH419_03995"
     CDS             3664..3822
                     /locus_tag="VH419_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5679 domain-containing protein"
                     /protein_id="HEY7042812.1"
                     /translation="MAETYSGEFYCVKCKAKREASGEVHVNDKGTRMAKAKCPVCGTN
                     LNRILGKA"
     gene            3923..4792
                     /locus_tag="VH419_04000"
     CDS             3923..4792
                     /locus_tag="VH419_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042813.1"
                     /translation="MSHPAPSEPVVDAARTPRRVQSWTRTWIVTLAAFGVALALVYAL
                     PLGDDDAARPDVQQMFVAGIAIAAVTTAALFAVIRLELRLPASVAVLCVVFNALVVGV
                     KFVAAPNAVYEANAKKVFEVDLSLNDSLGGWAVAGLVFVLYAAVLFLIYRVARRRSGL
                     VAQRLRDARSRRGVVVGTVAALVLLVAGLATGVGLYLAIIGLSAGGDYLGYVFGSGTA
                     LLVAVALALAASAATVAFGRAGELARAAEDVGILVSLFWICLLFLALYHVLWVVYILL
                     ITSMWPLKVVTPK"
     gene            4789..5835
                     /locus_tag="VH419_04005"
     CDS             4789..5835
                     /locus_tag="VH419_04005"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HEY7042814.1"
                     /translation="MTASSAPTEAAVPARRLGLAVRRLSLARLAWDMLRWRVALTMWL
                     FMLLAAAYHGVQDWPLDRLLALSLALGASYVVATTVNDIADVDIDRVNRLGGRGRPLV
                     TGDATRAELYRLNVVAALVALAFGSYVGWRGVAVVAASLVVAYVYSVPPLRISHRMLL
                     TAPVLTLAYVAAPYLHGLALAGVLPSTADLAFAGGLCCLFCSRILLKDFRDRAGDAAH
                     GKPTLALRFGKPVTCAIAFVALVVGSVLVASALKAPLPAVAVVGFGGCVGRQLWALVR
                     APDHRSEQVAIGIGARMGNGLLICLLAWLLLEAQGAAAATTCGVVTVIGLLYAATYVT
                     LVRHPDEVLVGYKG"
     gene            5965..6855
                     /locus_tag="VH419_04010"
     CDS             5965..6855
                     /locus_tag="VH419_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042815.1"
                     /translation="MRPQLRPGLQVLRRDVHTLQLGLDWPGVVVLEDSPALQAVLRAI
                     DGIRDVAAVVMTAARQAGVAESSCRAALEALIDCGAVVDQAQHLRGSVTEPAWAATWL
                     LAGPAGGAPDLAAAKDAHALHVVGSGQVADGVRALAARHDVRLAGSSADVLVVASDHE
                     PSRDVSDRVMADGRPHLWAVVRELVGVLGPFVQPGDTACLRCVDRSRARHDPAWPTLV
                     ESAAGRPGHVHACDPVLASLIAIWAVQEVSVWMSGLRPQTLGRTIEVPVGVGVVEAEH
                     HLAHPACGCGWSWSHDTMGA"
     gene            6852..8186
                     /locus_tag="VH419_04015"
     CDS             6852..8186
                     /locus_tag="VH419_04015"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012922898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HEY7042816.1"
                     /translation="MTDLPRKTVARTARLASLPLGFAGRATLGIGKRIGGAPAEAVAS
                     SVQQRTAEQLFKVLGELKGGAMKFGQAMSIFESALPESLVAPYRTTLTQLQDSAPPMP
                     AAVVHQVMAREFGGNWRSRFTSFDDNPAAAASIGQVHRAVWSDGQDVAVKIQYPGAGE
                     ALLSDLRQIGRAARLFAGWIPGIDVKPLVQELQARVAEELDYGLEADAQSRFADAFAD
                     DPEVVVPAVVAHTDQVLVSQWLESDRSLARLISDGSQEERDRYGEIYVRFLFSGPARA
                     GLLHADPHPGNFRILPDGRVGVVDYGAVARLPEGHLPLDIGVLLRHAAQDDYDAVLDG
                     LRRLGFVKSSVHVEVDELRAYLTPFVEPATTETFQFSREWMRGQFARINDPRAPGYTT
                     VLKINLPPEYLLIHRVWLGGIGVLSQLGAKAAFSRILAESLPGFADDDAEIP"
     gene            8733..9479
                     /locus_tag="VH419_04020"
     CDS             8733..9479
                     /locus_tag="VH419_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008356704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY7042817.1"
                     /translation="MNTLGGRVALVTGTSSGLGKQFAKALDGAGARVVLASRRHETDL
                     ELAETLLHEALAVRCDVRDVQDRAALVDAALQRHGRIDILVNNAGVAHSAPAEEEDLD
                     GVRHLLDTNLTAVFALSQLVAPHMFERNGGVIVNIASPAATTSLDRYGLAGYAASKAG
                     VVALTRELAAQWGRRGVRVNAIAPHFFPTATDGYLQDPELVGWLTARSPLGRTARIEE
                     IDGPLLFLCSDASSYVTGQTIVVDGGWTVY"
     gene            complement(9783..9980)
                     /locus_tag="VH419_04025"
     CDS             complement(9783..9980)
                     /locus_tag="VH419_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012922899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042818.1"
                     /translation="MVVCDYCGTPAPAGVSGEDQEPGTPHDVPFTWVTSVEDGKRRVF
                     CEHCAREHLRSIESKLDSEWW"
     gene            complement(10164..10373)
                     /locus_tag="VH419_04030"
     CDS             complement(10164..10373)
                     /locus_tag="VH419_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008364201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042819.1"
                     /translation="MTNTTSNTFSNRSTEMNLIQENLARAHIDARLREARELRRGHQL
                     HRARRLADKAERARQQARLLLARAL"
     gene            complement(10447..10734)
                     /locus_tag="VH419_04035"
     CDS             complement(10447..10734)
                     /locus_tag="VH419_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HEY7042820.1"
                     /translation="MTISVLDALIASEVTDANELPCRANDPELFFAESPADVELAKAL
                     CRSCPVRAECLSGAIDRHEPWGVWGGELLVQGVVVARKRPRGRPRKNEVAA"
     gene            complement(10844..11056)
                     /locus_tag="VH419_04040"
     CDS             complement(10844..11056)
                     /locus_tag="VH419_04040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042821.1"
                     /translation="MINITAIATIIPSCIGMPALREESVLGAPVKGRRMAVGTAAHTG
                     YTFGVQVAPAFLKDVSTGVRAWSPPI"
     gene            complement(11161..>12759)
                     /locus_tag="VH419_04045"
     CDS             complement(11161..>12759)
                     /locus_tag="VH419_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017934887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase UvrD2"
                     /protein_id="HEY7042822.1"
                     /translation="IDMEDVLLCAAAVLSDDERAAAAVRRQYQWFVVDEFQDVSPIQW
                     ALLGLWLGGRDSLCVVGDPAQTIYTFAGASADYLLHFAKTFPAATSLTLSRNYRSTPQ
                     VVATANALMTPQLGRAAVELRATREAGTAVSFREHSDEVSEAAAVATTIAKLVAGGVA
                     ASDIAVLFRINAQSESFEEALSERSVPFVVRGVERFFDRPEVRQATTLLRGASRAAAV
                     STDASVEPAGDRSLGEQVAAVLATMGWSVEAPEGRGNARDRWESLQALLALADEVASA
                     SPGVDLAGFVAELQRRADTQHAPVADGVTLATLHAAKGLEWPVVFCVGMHEGSMPFVY
                     AATRAEIDEERRLLYVGFTRARDQLHVSWAAARTPGGRATRGPSRFVAPLLDGQAGRR
                     GRSGGSTSGARRRTPAARLTTCRVCGRTLTDARERKLGRCFDCPSTYDEGLYDALREW
                     RRERAASEQVPAYCVFTDATMTALAEMRPPDVSALVRIPGIGRAKVDKYGAAIVDIVR
                     LSMGESDDFEPNDEQPRTFFEISP"
BASE COUNT         1879 a         4426 c         4406 g         2048 t
ORIGIN      
        1 cgtggtcgtg gtcacgcacg gtgccgacga agaccgccac cccgccggcc tgactgtcgg
       61 ccaccaaggc gtacaccgtc tgcacgtcca gcggcgtctc gctgatctcc agcagccgga
      121 tggggtcgct cacgcacccg actctaacgg cgttccgcac cgccgccgat cgagagtgcc
      181 gctggtcgag ctggccgctg atcgagcatc ccgctgatcg agcccgtcga gatcaaggac
      241 ggggtctcga caagctcgac cgccgggagc ccgctgatcg agcctgtcga gatcaagggc
      301 ggggtgtcga gcaggttgtg ccgagggtga agtcgtttcg ggctgtcgag ggtggccagt
      361 acgttgggag gcatggctga tgtcccaccc gaccgacccg acgagccccc gaccgacccg
      421 cccggcgacg agccgtcgcg cgaaccgagc gtgcccggca acccgttcgc gggtacgccg
      481 ttcgagcaga tcttcggcgg cctcgccggt ggcgacacca gcggcctgca ggcgatgttc
      541 ggccagctgc agcggatgtt cgcgccacac gaaggcgcgg tgaactggga cttcaccaaa
      601 gacctcgccc gccaggtcgt ggcccagcac agcgaccgct ccccggacgc cggcgacgtg
      661 tcccggctcg ccgacgtctc ccggctcgcc gagcactggc tcgacggcgt gacgagcctg
      721 cccgcggcca gcccgaccgc gcaggcatgg agccgcgccg cctgggtgga ggcgagcatg
      781 cctgtgtggc gacaactggt cgaaccgatc gccgagagcg tcgtcaaggc gatggggcag
      841 gcactcccca ccgaggcgca ggcgatggcc ggcccgatgc tcggcatcct caaccagatc
      901 ggtggcgcgt tgttcagcca gcaggtcggg caggcgatcg gcggcctctc ccaggaggtc
      961 gtcaccagca ccgacgtcgg cttccccgtc accgacgacc acacgccggc catcgtgctg
     1021 acgaacgctg cggagttcgc ctccgggctc ggcgtggacg agtccgacgt gctgctctac
     1081 ctcatgttgc gcgagtgcgc gcaccagcgc ctgttcctcc acgcgccctg gctacgggcg
     1141 cacatcttcg ccacgatcga ggactacggc cgcggcatca cgatcgacac gtccaagatc
     1201 gaagagaacg tccgcggcct cgacccgagc aatctcgagg ccatgcagga cgcgctcaac
     1261 ggtgggttgt tcgatctcga ccacacgccc gagcagcagg cggcgctcac ccggttggag
     1321 accctgctcg ccctggttga gggatgggtc gacgaggtgg tcggacaggc gaccgagaag
     1381 gcgatgccgc aggcacaggc gctccgggag gcggtacgcc gtcgccgcgc caccggcggt
     1441 cctgccgagg cgaccttcgc cgcgctcgtc ggcctcgagc tgcggccgcg acggctccgc
     1501 gaagcctccg ccctgttcgg cgcgctccgg gccgccgaag gtccggtcgc ccgcgacgcc
     1561 gtctgggccc accccgatgc gctgccgacc gcggccgacc tcgacgaccc gctggcctac
     1621 gcacagcgca cgaaggaggc cgacagcatc gacgtgctgt cacaggagtt cgacgaggcg
     1681 ctcaccaagt tcctggagtc gagccgctcc gacgcctcgt ccgagccagg ctcggccaca
     1741 gacgaacccg gagcaacgtc ctcagacgac ggcgacgaca ccgacgagtc gcccggttcg
     1801 gacacccgct gacgtcgcga cgagcgccga gttcacgctt gtcccgtcca gcgtcgtccc
     1861 gttcaccgct gtatgacgac gccgtctcgg cgctgaccag gtggcgcccc ccggacgccg
     1921 agcaggccag gctcaaggcg gcgtacctcg cccatctgga acggcacacc gatgggctca
     1981 gtcgcgactg ccaccccgac cacatcaccg ccagtgcgct ggtggtgagc cacgaccgac
     2041 ggcaggtgct gttgaacctg catcgcaagt acgagatctg ggtccagttc ggcgggcact
     2101 gcgaggccgc tgaccgccgg ctgcacgagg cggcgttgcg agaggcggcc gaggagtcgg
     2161 gcatcgacgg actccgactg cgcgacgaga cgccggcgca gctgagcaga cacgaggtca
     2221 ggtgtggccc actccggccg tcgcaccatc tcgacgtccg ctatgttgcg gttgctccac
     2281 aatcggcgca gcccgagccc accgcggagt ccttggcggt cacgtggttc gatccggcag
     2341 atctgcccgc cggagtcgac gggccgctgc gtgaactcat cgcctgggcg ctggcgtgac
     2401 cggagcccga ccgaacgcca acgaggcccc caggacgcgg atgccgctcg ccttcccctt
     2461 gcgaacgcct ccgttttccc gggggcctca tcgatggtgc cgacgctagg tcgaggccaa
     2521 cggcgccgtc aacgcacaac cggacatcgc cgtccacgcc tgtggacaac cctgtggaca
     2581 ggttgtggga agccgcaccg gagctgtgga caggctgtgg gcaacgtggg ctcaggctgt
     2641 gcagatcggc gtcacgtccg gtcgtgtcgc cgcctgacca ggcactttgc cacgctcact
     2701 gtccacgaca tgtggacgag aaaaaccgtg tcgcgcggca atcactgcgt gccaccctcg
     2761 tcgtaccacc aatcccgggg ttccaccgat taggttgtgc gcatggaaaa gccgtcgtct
     2821 gcggtcgagg tacgccgctc gcggcgacgt aagcgcacgg tgtcggccta tcgcgacggc
     2881 gagaccatcg tcgtgatgat cccggcccgg atgacccgcg ccgaggagca ggagtgggtg
     2941 gccaccatgg tcgaccgggt cactcgtgcc gaacgccgac gccggcccag cgaccgggat
     3001 ctcgagcagc gtgccgcccg gctctcccgc aagtatctcg acggactggc cgaacccacc
     3061 tcggtgcggt gggtgacgaa ccagcaggct cggtgggggt cgtgtacgcc gaccgacggg
     3121 acgatccggc tctccgaacg cctgcagggc atgccggcat acgtcatcga ctacgtgctg
     3181 ctccacgagc tcacccacct gctcgcgccc ggtcacgacg acgacttctg gtcctggctg
     3241 cagcgctacg acctgacgga acgggcccgg ggtttcctcg aaggcgtgtc gaacgcgatc
     3301 cacctcttgc ccgcgaccgg taccgcgccg gacgtcgatc cggtcggcga cctcggcgac
     3361 ctcgacgacc tcgacgatct ggacacggcc gcggccgagc gctgaccgcc gagcgagcag
     3421 gaaaggcgat gacttcttgt ccattatggg gcggaatatc ccacaggttg agcgtgaatc
     3481 cgaggtcgga cgctgtggcg aataccgcac cgccccacgc ctgacgtagt gtgacgcgag
     3541 gtcggcggtg gcggcaccca cccccgcccg gcgggactct cgaccggagt tctccgtcga
     3601 gatgttctgc acagcgatca tcgggacccc cggtggccgc ctagagacga acaggaggcc
     3661 gtcatggcgg agacctacag cggcgagttc tactgcgtga agtgcaaggc caagcgcgaa
     3721 gccagcggcg aggtgcacgt caacgacaag ggcacccgga tggccaaggc caagtgcccg
     3781 gtctgcggca cgaacctgaa ccgcatcctc ggcaaggcct gatcagccgg acacacacgc
     3841 cagcggcccg cacgggccgt ggagggcagg gcctcgctgc ggggccctgc ctttcgcgcg
     3901 tccagcctca ccaggagcac ccatgtcgca cccagcaccg tcggagccgg tcgtcgacgc
     3961 ggcgcgtacg ccgcggcgcg tccagtcgtg gacccgcacg tggatcgtca cgttggccgc
     4021 cttcggcgtc gcactcgcct tggtctacgc gctgccgctc ggcgacgacg acgccgcgcg
     4081 gccggacgtg cagcagatgt tcgtcgccgg catcgccatc gccgcggtga ccaccgcggc
     4141 gctgttcgcg gtcattcggc tcgagctgcg gctcccggcc agcgtcgcgg tgctgtgtgt
     4201 cgtgttcaac gcgctcgtcg tcggggtgaa gttcgtcgcc gcaccgaacg ccgtctacga
     4261 agccaacgcg aagaaggtct tcgaggtcga cctctcgctc aacgacagtt tgggtggatg
     4321 ggccgtcgcc ggcttggtct tcgtcctgta tgccgcggtg ctcttcctca tttaccgcgt
     4381 tgcgcgcagg cgcagcggac tggtcgcgca acggctgcgc gatgcacgtt cgcggcgggg
     4441 cgtcgtcgtc ggcacggtgg cggccctcgt gctgctggtc gccggtctcg ccaccggcgt
     4501 cgggctctac ctcgcgatca tcgggctctc cgctggcggt gactacctcg ggtacgtgtt
     4561 cggctccgga acggccctgc tcgtcgccgt tgccttggcg ttggccgcgt ccgcagccac
     4621 cgtggcgttc ggccgggctg gggagctggc tcgagcggcc gaggacgtcg gcatcctcgt
     4681 ctcgctgttc tggatctgcc tgctcttcct cgcgctctac cacgtgctgt gggtcgtcta
     4741 catcctgctg atcacctcga tgtggccgtt gaaggtcgtc acgccgaaat gaccgcgtcg
     4801 agcgcgccga ccgaagccgc cgtcccggcc cggcggctgg gtctggcggt ccgccggctg
     4861 agcctggcgc gactggcctg ggacatgctg cgctggcggg tcgcgctgac catgtggctc
     4921 ttcatgctgc tggccgcggc gtaccacggc gttcaggact ggccgctcga ccggctgttg
     4981 gcgctctcat tggcgctcgg cgcgtcgtac gtcgtcgcca ccaccgtcaa cgacatcgcc
     5041 gacgtggaca tcgaccgcgt caaccggctg ggtggtcgag gccgcccgct ggtgaccggt
     5101 gacgcaacgc gggcggagct ctaccggctc aacgtcgtcg ccgcgctggt cgcattggcg
     5161 ttcggcagct acgtcggttg gcgcggggtg gccgtcgtgg cggcgtcgct ggtcgtcgcc
     5221 tacgtctact cggtgccgcc actgcggatc tcccatcgga tgttgttgac cgctccggtg
     5281 ctgacgttgg cgtacgtcgc cgcgccctac ctgcacgggc tcgcgctggc aggcgtgctg
     5341 ccgtcgacgg ccgacctggc cttcgccggt gggctgtgtt gcctcttctg ctcgcgcatc
     5401 ctgctcaagg acttccgcga ccgcgccggc gatgcggcgc acgggaagcc gacgctcgca
     5461 ctgcggttcg gcaaaccggt cacgtgcgcg atcgcgttcg tcgcgctcgt cgtcgggtcg
     5521 gtcctcgtcg cgtccgcgct caaggccccg cttccggcgg tcgcggtggt cgggttcggc
     5581 gggtgcgtcg gccggcagct gtgggcgctc gtccgagcac ctgaccaccg cagcgagcag
     5641 gtggcgatcg gcatcggtgc gcggatgggc aatggcctgc tgatctgcct gctcgcctgg
     5701 ctgctgctcg aagcgcaggg cgcggctgcg gcgacgacgt gcggtgtcgt gacggtgatc
     5761 gggctgttgt acgccgcgac gtacgtgacg ctcgtccgtc atcccgacga ggtgctcgtc
     5821 ggctacaagg gctgacgcac cgatcggccg tggctgtgga cggcggccac gccagggcgg
     5881 gctgcgctgg catgctgcct gcgttccgcc ccctcttcgg tcggccacgg tgcgcgccgg
     5941 acccaggcag cagaggagtt tcccgtgcgc ccacagctcc ggcccggtct gcaggtcctt
     6001 cgacgcgacg tccacaccct gcagctcggc ctggactggc ccggcgtcgt cgtcctggag
     6061 gactcgccgg cgctccaggc ggtgctgcga gccatcgacg ggatccgtga cgtcgccgcc
     6121 gttgtgatga ccgccgcgcg tcaggccggc gttgccgagt cgtcgtgccg cgccgccctc
     6181 gaggcgttga tcgactgcgg tgccgtggtg gatcaggccc agcacctgcg cggcagcgtg
     6241 accgagcccg cctgggccgc gacctggctg ctggcgggac ccgccggtgg cgcgcccgac
     6301 ctggccgccg cgaaggacgc tcacgcactc cacgtggtgg gctcgggcca ggtcgccgac
     6361 ggcgtacgcg ctctggctgc ccgacacgat gtgcggctgg ccggaagcag tgcggacgtc
     6421 ctcgtcgtcg ccagcgacca cgagccgtcc cgcgacgtgt cggaccgcgt gatggcggat
     6481 gggcgcccgc acctgtgggc tgtcgtccgg gagctggtcg gcgtactcgg accgttcgtc
     6541 cagcccggcg acaccgcgtg ccttcggtgt gtcgaccggt cccgagcccg gcacgacccc
     6601 gcctggccga cgttggtcga gtctgctgct ggccggcccg gtcacgtcca cgcatgcgac
     6661 ccggtgctgg cctcgctgat cgccatctgg gcggtccagg aagtgtcggt atggatgtcg
     6721 gggcttcgtc cccagacgtt gggccgcacg atcgaggtgc cggtcggcgt cggcgtggtg
     6781 gaggccgaac atcacctggc acatcccgca tgtggctgtg ggtggtcgtg gtcgcacgac
     6841 acaatggggg cgtgaccgac cttccccgca agaccgttgc gcgtactgcg cggctggcgt
     6901 cgttgccctt ggggttcgcc ggtcgtgcga cgttggggat cggcaagcgg atcggtggcg
     6961 ctcccgccga ggcggtcgcg tcgtcggtcc agcagcgcac cgccgaacag ctcttcaagg
     7021 tgttggggga gctcaagggc ggggcgatga agttcggaca ggcgatgtcg atcttcgagt
     7081 cggcgctgcc ggagtcgctg gtcgcgccgt accgaacgac gctcacgcag ctgcaggact
     7141 cggcgccacc gatgccggcc gcggtggtcc accaggtgat ggcccgtgag ttcggtggca
     7201 actggcgaag ccgcttcacg tcgttcgacg acaacccggc ggccgctgcg tcgatcgggc
     7261 aggtccaccg agcggtgtgg tcggacggtc aggacgtggc ggtgaagatc cagtaccccg
     7321 gcgcgggtga ggcgctgctc agcgacctgc gccagatcgg tcgggcagcg cggctgttcg
     7381 ccgggtggat cccgggtatc gacgtcaagc cgttggtgca ggagctgcag gcgagggtcg
     7441 ccgaagagct cgactacggt ctggaggctg acgctcaaag ccggttcgcc gacgcgttcg
     7501 ccgacgatcc cgaggtcgtg gtccctgccg tcgtcgcgca caccgaccag gtgctcgtct
     7561 cgcagtggct ggagtccgac cggtcactgg cccggctcat ctccgacggg tcgcaggagg
     7621 agcgcgaccg gtacggcgag atctacgtcc ggttcctgtt ctccggacca gcccgcgccg
     7681 gcctgctcca tgcggatccg catccgggca acttccgcat cctgccggac ggtcgggtgg
     7741 gcgttgtcga ctacggtgcg gtggcgcgcc tgcccgaagg tcacctgccg ctcgacatcg
     7801 gcgtactgct gcgccatgcg gcgcaggacg actacgacgc cgtgctcgac gggctgcgcc
     7861 gcctcggctt cgtcaagtcg tcggtgcacg tcgaggtcga cgagctgcgt gcctacctca
     7921 cgccgttcgt ggagccggca accaccgaga cgttccagtt cagccgggag tggatgcgcg
     7981 ggcagttcgc ccggatcaac gacccgcggg cgcccggcta caccaccgtg ctgaagatca
     8041 acctgccgcc ggagtacctg ctcatccacc gggtctggct cggcggaatc ggcgtcctgt
     8101 cacaactcgg cgcgaaggcg gccttctctc gcatcctcgc cgagtccctc cccggcttcg
     8161 ccgacgacga cgccgagatc ccctgacccc gcccccgccg aggtggcagg ggtgtcaggt
     8221 gggtccccgc tcgtcgcgag cggggtcgag gtggatgagc tgccaggcgg aggaggagat
     8281 cgatgcccag catcgggcga cgacgacaac gccgcagatc gccgcctcgg gcacgcgcag
     8341 cagagcatgg gcccacctga ggcccctcaa tttgttgcgt tttgacggcg tgtcgaacgc
     8401 cgatcgccac ctcggccgta ggccgctgac gacctcgtga cacggaggca gtgcggggcg
     8461 taccccgccg ctcgaagaac gaagaccact cagaccccgg ctcccttctg atgcgggtct
     8521 ccctcaggat cgtggaggct gcgcatcgtc gctgagtcga agatgtgctc gggccggcca
     8581 gcaccaaccc tcgcgagtcg actctgaaca tgtccggtct gcccttgttc acggttggga
     8641 gccatggtgg gcatgtggtt tgctgcgcct gtggactggc gcaggattat gttgatcggc
     8701 gaggagcgcg gagccgctga tgggtgggtg tcttgaacac cttgggcggc cgggtggcgt
     8761 tggtgaccgg gacctcgtcc ggcctcggca aacagttcgc aaaggcgttg gacggcgccg
     8821 gagcgcgcgt cgtccttgcc tcgcggcgtc acgaaacgga cctcgagctg gccgagacgc
     8881 tgctgcacga ggcgctggcc gtgcggtgcg acgtgcgtga cgtccaggat cgggccgcgc
     8941 tggtggatgc ggcgctccag cgtcatggcc ggattgacat cctggtcaac aatgccggcg
     9001 ttgcgcactc ggctccggcg gaagaagagg atctcgatgg cgtccgacac ctgctggaca
     9061 ccaacctcac cgcagtgttc gcgctctcgc aactggtggc gccgcacatg ttcgaacgca
     9121 acggtggcgt gatcgtgaac atcgcttctc ccgcagcgac caccagcctc gaccgctacg
     9181 gcctcgccgg gtatgccgca agcaaagcgg gggtcgtcgc cctcacgcga gagcttgcgg
     9241 cacaatgggg gcgtcgcggc gtacgcgtca acgccatcgc accgcatttc ttccctaccg
     9301 cgacggatgg ctatctgcag gacccggaac tcgtcggctg gctgacggca cgctcacctc
     9361 tcggccggac agcccggatc gaggagatcg acgggccgct gctcttcctc tgcagcgacg
     9421 cctcgtcgta cgtcaccgga cagaccatcg tcgtcgacgg tggatggacg gtgtactgac
     9481 cgacggtatg gccagggagg gcccgccccg gcccgttgcc cgacgcacgc ggtagcgcca
     9541 cgacagcctc cactgtgcgg ggcgggccag ggtcctgcac gacgcggcgt cgatcccgcg
     9601 gcacttcctt cgcctcgctt cccatcgcca gcggcgccga cgtcatagtc gttcggccaa
     9661 ggatgaggca cgagcgcgaa gacaacgctc gacaactacg gtcatccgtc ggctcgtgtg
     9721 aacagcccgc ggactccgta agacggctca gcacaaggtg cctggtcaga gcgagcggtt
     9781 tcctaccacc actcggagtc cagcttggat tcgatgctgc ggaggtgttc gcgagcgcag
     9841 tgttcgcaga agacccgccg tttgccgtct tcgaccgacg tgacccaggt gaaaggaacg
     9901 tcgtgcggcg taccgggttc ctggtcctcg ccggagacgc ctgccggcgc tggtgtcccg
     9961 cagtagtcgc acacgaccat ggtccaagcc aaccacgcgg cccaaggccg gaccagcctc
    10021 ggcgaggtgg caatgtcggc acgacacgcc ggcaaaatcc aacgaaacct gccacctcgg
    10081 cgaagaagaa gaaacggcgc ccgggccctc cacgtcgggt ggaggggccg ggcgccgtcg
    10141 acgccgtctc atgaccgcct aggtcacaga gcgcgggcga gaaggaggcg ggcctgctgg
    10201 cgggcacgct cggccttgtc ggccaggcga cgtgcacggt gcagctggtg gcctcgacgc
    10261 agttcgcgcg cctcccgaag gcgcgcgtct atatgggcgc gtgccaggtt ttcttgaatg
    10321 agattcatct cagtgctcct gttcgaaaaa gtgttgctgg tcgtgttggt catcgaaatt
    10381 cgtttctctc agttcgcttg ggcgagccgg tttggtctcg cttgcctggt gctactcgcg
    10441 ctgcggtcag gcagcaacct cgttcttacg gggcctaccc cttggccgct tgcgagcgac
    10501 aaccactcct tggacgagta gttctccgcc ccacaccccc cacggttcat ggcggtcgat
    10561 cgccccggag aggcattcgg cccgcacggg gcatgaacgg cacagcgcct tggccagctc
    10621 gacgtctgcc ggcgactctg cgaagaacag ctccgggtcg ttcgcccggc agggcagctc
    10681 gttcgcgtcg gtcacctcac tcgcgatgag tgcgtcgagg acgctgatag tcatgtgtgt
    10741 cacctccttg tgaccttcgt cgttcggact ggggtaaacg aaaaagccgc ggattccctg
    10801 ttgggagtcc gcggcttgga ggtggccgac cagagcgttc actctagatc ggtgggctcc
    10861 aagcacggac gcccgtggag acgtccttga ggaatgccgg cgcgacctgc acaccaaagg
    10921 tgtagcccgt gtgggcggcc gtaccgacgg ccatgcgacg acccttgaca ggtgcaccga
    10981 ggacagactc ctcccggagg gcaggcatgc cgatgcacga cgggatgatc gtcgcgatcg
    11041 ccgtgatgtt gatcattgct gcgcctcctt tcggtgagtc ctggttgtca gggtgagcag
    11101 agtcgagtgc cctctgcgtc caagcagcgt acgcgcgcgc ggaagcgggg tgcaaatgaa
    11161 ttacggggaa atctcgaaaa aagttcgagg ttgttcgtcg ttcggttcaa aatcgtcgga
    11221 ttcgcccatg ctgagccgca cgatgtcgac gatcgcagct ccgtacttgt cgaccttcgc
    11281 ccgcccgatc cccgggattc ggacgagtgc agagacgtcc ggcggtcgca tctcggccag
    11341 cgccgtcatg gtggcgtcgg tgaagacgca gtacgccgga acctgctcgc tggctgctcg
    11401 ctctcgccgc cactcccgca gcgcgtcgta gaggccttcg tcgtacgtgc tcggacagtc
    11461 gaagcagcgg ccgagcttgc gctcgcgcgc gtcggtcagc gtccgaccgc agacccggca
    11521 cgtggtgagg cgggcagccg gcgtacgacg ccgggcgccg gaagtcgaac cgcccgaccg
    11581 accgcgacgg ccggcctgcc cgtcgagcag cggcgcgacg aaccggctgg gaccacgcgt
    11641 tgcccttccg ccgggggtgc gcgccgccgc ccacgacacg tgcagctggt cgcgtgccct
    11701 cgtgaagccg acgtagagca gcctgcgctc ctcgtcgatc tcggcgcgag tggcggcgta
    11761 cacgaacggc atcgaccctt cgtgcatgcc gacacagaac accaccggcc actcgagccc
    11821 cttcgccgcg tgcaaggtgg ccagcgtgac gccgtcggcg accggagcat gttgcgtatc
    11881 cgctcgacgc tgcagctccg cgacgaagcc ggccaggtcg acgccgggac tcgccgacgc
    11941 cacctcgtcg gcgagcgcga ggagggcctg cagcgactcc cagcggtcgc gggcgttgcc
    12001 gcggccttcg ggtgcctcga cggaccagcc catcgtcgcc agcacagcgg cgacctgctc
    12061 gcccaggctg cggtccccgg ccggctcgac ggacgcgtcg gtgctgacgg ccgccgcccg
    12121 gctggcgcct cgaagcagcg tcgtggcctg acgcacctcg ggacggtcga agaaccgctc
    12181 gacgccacgt acgacgaacg gcaccgacct ttcggacagc gcctcttcga agctctccga
    12241 ctgagcgttg atccggaaca gcaccgcgat gtcactggcg gccactccgc cggcgaccag
    12301 cttcgcgatc gtggtcgcga cggcggcagc ctcggacacc tcgtcactgt gctcgcggaa
    12361 gctcaccgcc gtgccggctt cccgcgtcgc cctgagctcg accgcagccc ggcccagctg
    12421 cggcgtcatc aacgcgttgg cggtcgcgac cacctgcggg gtcgagcggt agttgcggct
    12481 gagcgtcagc gacgtcgcgg ccggaaacgt cttggcgaag tggagcagat agtcggccga
    12541 ggcgccggcg aaggtgtaga tcgtctgggc cgggtcgccg acgacgcaca gcgagtcacg
    12601 gccgccgagc cacaggccga gcagcgccca ctggatcggg ctgacgtcct ggaactcgtc
    12661 gacgacgaac cactggtact ggcggcgcac cgctgctgcc gcgcgctcgt cgtcgctcag
    12721 caccgccgcc gcgcacagca gcacgtcctc catgtcgat
//
LOCUS       DATBFZ010000136        11534 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_5624583, whole genome shotgun sequence.
ACCESSION   DATBFZ010000136 DATBFZ010000000
VERSION     DATBFZ010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 11534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11534
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5624583"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..960)
                     /locus_tag="VH419_04050"
     CDS             complement(19..960)
                     /locus_tag="VH419_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020576955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEY7042823.1"
                     /translation="MTPKVPSSADVEAAAFRLGSSIRRTPVLELDLREHGVESHVHLK
                     LELTQHTGSFKARGALNSVLSLPKDVTGVCAASGGNHAAAVSWAARRAGLVADVFVPA
                     EATPAKLDRIRSYGGRIHAVDGHVKEALEACRAYSEEAGVPLLHPYDTFETVSGAGTT
                     GLEIEEQVPDVDLVLVGCGGGGLYSGLAISLEGIARVQPVEPELCPDLACALAERGPV
                     PVAVGGLAADSLGAPYIGDIAYAVASKHAVEPVLIPEASILAARMLLWESTRVLAEPG
                     ACVALGSVLTGAVRVDPGQPVVVVVSGGNNETFPTVP"
     gene            complement(1007..1957)
                     /locus_tag="VH419_04055"
     CDS             complement(1007..1957)
                     /locus_tag="VH419_04055"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEY7042824.1"
                     /translation="MADKGDAETGFAAGAKAKLAEARRRLPFLDHVIATQEHYMKVEG
                     TVLAGAVTYFGFLSFFPLLAIAFGVVGYVSASFPDAREHLVTAIEGLFPGIVSANGGH
                     GTISLKQIERAATTTGIVGLLVLLYSGLGWVYGLRAALADTFEIPRSQKRSFVLGKIG
                     DLGVLALLGVVMIVSVGISGTVESAAGSIVSFAGLDETVVGSPLVWGVGLLLSLAAST
                     LLFSIMYKLLANPDLPRRPILEGAVFAAIAFEVLKFVVVNIIGGVGGTSVAALAISVT
                     LVVWINCCSRLVVYGAAWAMTSRRSSPARVGDSEPPRVTD"
     gene            complement(2096..2614)
                     /locus_tag="VH419_04060"
     CDS             complement(2096..2614)
                     /locus_tag="VH419_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020393009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HEY7042825.1"
                     /translation="MSTIGVAVAIPEPWATQLQQQRAAFGDPQADAIPTHITLVPPTE
                     IDGNLATVDEHLAKVASDQRPFRLRLRGTATFRPVSPVVFIVVTEGISACEVLAAAAR
                     SGPLAQELRFPYHPHVTVAHHLDEAALDLAYDSLADFDCAFEVAEFHLYLHGPDGVWR
                     PHRTFPLGGSAD"
     gene            complement(2656..3681)
                     /gene="trpS"
                     /locus_tag="VH419_04065"
     CDS             complement(2656..3681)
                     /gene="trpS"
                     /locus_tag="VH419_04065"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEY7042826.1"
                     /translation="MTRMSTPAVPTRPRVLSGIQPTADSFHLGNYLGALRQWVALQFD
                     HDAFYCIVDLHAITVDQEPKLLRERTLVSAAQLIAMGLDPAQCTLFVQSHVPEHAQLA
                     WVLGCITGFGEASRMTQFKDKSARGGQATVGLFTYPILQAADILLYQANQVPVGEDQR
                     QHLELTRDLGARFNRRFGETFVLPEPHIIKGTAKITDLQEPTAKMSKSASSPAGLIEL
                     LDEPSRSAKKIRSAVTDSGREVYYDEEEKPGVSNLLTIYSALSGRSVDDVAAEYDGRG
                     YGDLKKDLGEIVVDLVTPFRARTIELLEDRAELDAVLRTGADRARAVAAETLATAYDR
                     IGFVPAR"
     gene            3792..4298
                     /locus_tag="VH419_04070"
     CDS             3792..4298
                     /locus_tag="VH419_04070"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigE family RNA polymerase sigma factor"
                     /protein_id="HEY7042827.1"
                     /translation="MAEHDFVREVYDAAYPRLVAQMLVLCGDQGDAEDVVQEAFVKAM
                     SQRSAFGRLDNPEAWLRTVALNLLRNRWRRSAVFRRLMPRLPGSQHQVELSPDRVAVI
                     EALRKLDQPLREVVTLHHIGDLPVEEVARTLGIPSGTVKARLVRGRAVLAEHLAETTA
                     DREENHHV"
     gene            4291..5628
                     /locus_tag="VH419_04075"
     CDS             4291..5628
                     /locus_tag="VH419_04075"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEY7042828.1"
                     /translation="MSDPTDLDTRLATLAHDVRGKVVAPSYDDLVSTSRRRRRSSALL
                     STVATLVVVGLAWAGVHGLHDDTSTPTQPANGRHLTTEPIPRPTHESPPAGSRRLTAE
                     QIVDASDSYLVAFAESGADPEAQVSVWRCREQPNCPGWRGGIAVTDDDFGTRTVIDLP
                     RNANPVAQDVGDKMFLVGDAPNGVLLRPDGTTVAMTLYRTTGPLASGEVLVRVHTGPA
                     TFGGLDPQTGRVHLLAVPRSDGVDLQGNGSWLWGVAFTDTADSVMQRVIWSDDGGTTW
                     NTKWNTRVTRLDANRFLSPLPSQRVGTMPFLVTSDGATLTPYGGVYGSQDGGQTWQSY
                     PGWADPTGYVGTGLIQPDGSLLLNVIDWSDAGPHKPSRQPIGLYASSGTDWGTLQFVQ
                     DLPSTPGSTDLRAMYAVLTYVPDGQQARVWIYGNGHVFESGSGIDTWREIPVR"
     gene            complement(5932..7245)
                     /locus_tag="VH419_04080"
     CDS             complement(5932..7245)
                     /locus_tag="VH419_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020392994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEY7042829.1"
                     /translation="MSGTLRDIALVLLFILISSFFVLAEMALVSLRDSQAKALEERDG
                     RRGAIVAKLNADPNRFLAAVQVGVTLAGFLSAAFGGVTLASRLSPKLEDLGLSSGVAD
                     TTALVLITLAISYVSLVVGELAPKRLALQRAESISLTVAPTVDRIAKLARPVIWLLSV
                     STNLVVRLVGGDPTAQREQITDAEIRELVSGSETLGAEERQIVEEVFEAGDRQIREVM
                     VPRTEVDFIPAAMPAYKAAQFALERPHSRYPVIGDSSDDVIGFVHVRDLFNRELSRRS
                     VRVREIARDVLLLPATRQILSALSDMRREGAHVAIVADEYGGTAGIITLEDLVEELVG
                     DIKDEYDVDEPETTRLVGGELEVDGLLNLDDFEDETGLTLPDGPYETVAGYIVQQLGH
                     LPTVGETTSFDGHTLTVVELDGRRVSRVKVKSTAPPATASPAEFA"
     gene            7423..8553
                     /locus_tag="VH419_04085"
     CDS             7423..8553
                     /locus_tag="VH419_04085"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY7042830.1"
                     /translation="MEFPDDVPVLTNGDVTLRAHRTEDVEGVFEQCTDPVSVRWTTVP
                     LDYTAEMAREFVTARIPAMWADDTEWLFAIEATHHDGHRRFGGTLSLRAEGHRRAEIA
                     FGAHPGVRGHGVMTTAVNLLLDWGFHERDLETIIWLAERGNFASRRVAWKSGFTFGGV
                     LPKWLDHRGTLVDAWTGTLHRTDAREPTHTWYDVPVLTGESVVVRPQRLDDADRIVEA
                     CSDEITQYWVPSLPSPFTRDDAVDSITSGAEAAAQGNHLQWAVADRHTDQLVAVVGLP
                     RIKRGNAEVGYWTHPAARGRGVMKDALGQLIRHAFTAVERGGLGLHRLYVRTGVANVA
                     SQRVATANGFVPRGTERQVEQLRDGTYTDFAFLDLLRDEWEG"
     gene            8558..9694
                     /locus_tag="VH419_04090"
     CDS             8558..9694
                     /locus_tag="VH419_04090"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY7042831.1"
                     /translation="MRYPDDVPVLTEGTVTLRAHREDDLGGMYEQCIDPVSQRFTSVP
                     VPYTRAEAQTFLDSRRTAWEKDQHWSFAIESEWGVGPSGFSGSIGLRHHAPGLAEIGF
                     GAHPTARGHGVTTRAVKLLADWAFGEQGIRTIRWQAQVGNTASRRVAWKTGFTFEGAS
                     RSSLVQRGVLHDAWRATLLATDTREPKTRWLDTPTLDGDRVRLRPLRPDDVQRYLETN
                     NDPETLVWLGTIPFPHDSERFHRHLARASTGPSEGASVEWAMAEVGRDEYVGTVTLFG
                     MTGLDYKSAEVGYRTHPDSRGKGFLKEALRLAIGHAFTPGEDGGIGLERISLNAGADN
                     EGSLRAARACGFTETGRDRQCYDLYDGSVVDLIRFDLLRSEYAG"
     gene            9713..10786
                     /gene="galK"
                     /locus_tag="VH419_04095"
     CDS             9713..10786
                     /gene="galK"
                     /locus_tag="VH419_04095"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020576940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEY7042832.1"
                     /translation="MVTTWRAPGRVNLIGEHVDYADGLCLPFALPLTTTATVSARPGH
                     DVTVTSAGKTETFPVECGPGDVDGWAAYIAGVIWALRQDGAELPGVHIEVASDVPVGA
                     GLSSSAALECAVAGAIADEAGLEIAPVDLAARARRAENDFVGVPSGPMDQLASVLGSD
                     GHAVLIDCRDLSHRLVPFDPEADGLRLLVIDTHAHHTHAGGEYAERREQSESARRMLS
                     LSSLREAGLDDLDGIDAPVIRRRAHHIVTEIARVSDVVEILDAGQPQDIGPALTASHE
                     SLRDDFEVSCDELDAAVDAALTAGALGARMTGGGFGGCAIALVRETDEAVVTEHVVST
                     YASRGWTRPTIFPARPSAGAHRV"
     gene            complement(10841..>11534)
                     /locus_tag="VH419_04100"
     CDS             complement(10841..>11534)
                     /locus_tag="VH419_04100"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007079419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEY7042833.1"
                     /translation="VLPLVVAARDRYEAETGRRTPVVAGNVVSAAGAHELVKAGADVV
                     KVGVGPGAMCTTRMMTGVGRPQFSAVLDCASAAADSGATVWADGGVKYPRDVALGLAA
                     GASSVMIGSWFAGTYESAGDLASDADGRLYKVSFGMASARAVRQRTREQSAFDRARSA
                     LFEEGISSSRMYLDPARPGVEDLIDMIVAGVRSAFTYAGASTIEEFHERAVVGVQSAA
                     GYEEGRPRPTSW"
BASE COUNT         1913 a         3974 c         3893 g         1754 t
ORIGIN      
        1 acagtacgcg cggcccgctc agggcactgt ggggaaagtc tcgttgttgc cgccggagac
       61 gacgaccacg accggttgcc ctgggtcgac gcggaccgcg ccggtcagca ccgagccgag
      121 tgcgacgcag gcgccgggtt cggcgagcac ccgcgtcgac tcccacagga gcatccgggc
      181 ggccaggatc gacgcttcgg ggatcagcac gggttcgacc gcgtgcttgg acgcgacggc
      241 gtaggcgatg tcgccgatgt acggcgcgcc gagcgagtcc gcggccaggc cgccgacggc
      301 aactggcacc ggtccacgct cggccaacgc acaggcgagg tctgggcaca gctcgggctc
      361 caccggctgc acacgcgcaa tgccctccag cgagatcgcc agaccggagt agagcccgcc
      421 gccgccgcag ccgaccagca ccaggtcgac gtccggtacc tgttcctcga tctcgagtcc
      481 ggtcgtcccg gccccgctga ccgtttcgaa cgtgtcgtag ggatgcaaca gtggaacgcc
      541 ggcctcctcg gagtacgcgc ggcacgcctc caatgcctcc ttgacatgac cgtcgacggc
      601 gtggatgcga cccccgtacg aacggatgcg gtcgagcttc gcaggcgtcg cctcagccgg
      661 tacgaacacg tccgccacca ggccggcgcg gcgcgctgcc caagacacag cggctgcatg
      721 gttgcccccg gacgcggcac agacaccggt gacgtccttc ggaagcgaca gcaccgagtt
      781 gagggcacct ctggccttga acgacccggt gtgctgagtc agctccagct tgaggtggac
      841 gtgggactcg acgccgtgct cgcgaaggtc cagctccagc acgggggtac gccgaatcga
      901 ggagccgagc cggaaggcgg ccgcctcgac gtcggcgctg ctaggcacct tcggcgtcat
      961 gctgcgaggg cggtgcgaag gtcgatcgga acggctgtca cgcgcctcag tctgtcaccc
     1021 gaggcggttc cgaatctccg acccgcgcgg gcgacgaacg gcgagacgtc atcgcccagg
     1081 ccgcaccgta gacgacgagc cgtgagcagc agttgatcca gaccaccaac gtgaccgaga
     1141 tggcgagcgc tgcaacagac gtgcccccga caccgccgat gatgttcacc accacgaact
     1201 tcagcacctc gaaggcaatc gccgcgaaca ccgcgccctc gaggatcggt cgccgcggca
     1261 ggtcagggtt cgcgagcagc ttgtacatga tcgagaacaa cagcgtgctc gcggcgaggc
     1321 tcagcagcag gccgaccccc cagacgagcg gtgagccgac gacggtttcg tcgagtccgg
     1381 cgaaggagac gatcgacccg gcggcgctct cgacggttcc gctgatgccg accgacacga
     1441 tcatcacgac accgagaagc gccagcacgc ccaggtcgcc gatcttgccg aggacgaagg
     1501 agcgcttctg tgaccggggt atctcgaacg tgtcggccag cgccgcgcgc agcccgtaca
     1561 cccagccgag cccggagtac agcagcacca gcaggccgac gattcccgtc gtggtcgcgg
     1621 cccgctcgat ctgtttcagg ctgatcgtgc cgtgcccgcc gttggcgctg acgatgcctg
     1681 gaaacagtcc ctcgatcgcg gtgacaaggt gctcccgggc atcagggaac gaggcactga
     1741 cgtagccaac cacgccgaac gcgatggcga gcagcgggaa gaacgagagg aagccgaagt
     1801 aggtgacggc tccggcgagc accgtccctt cgaccttcat gtagtgctcc tgcgtcgcga
     1861 tcacgtggtc gaggaagggg agcctgcgtc gtgcctcagc cagcttcgcc ttcgcgcctg
     1921 ccgcgaaccc tgtctcagcg tcacccttgt ccgccatccc ctagtcctac cggaccttcg
     1981 gccgatcgag cctgtcggtg accggatcca agccaccgag tcgtcggttc ccggctcgag
     2041 aagccacctc ggctccctgg atctcgacac gctcgatccg cggtgctcga cagactcaat
     2101 cggcggatcc gccgagcggg aaggtacggt gggggcgcca cacgccgtcc ggaccgtgca
     2161 ggtagaggtg gaactcggcg acctcgaacg cacagtcgaa gtccgcgaga ctgtcgtacg
     2221 cgaggtcgag tgccgcttcg tcgaggtggt gcgcgacggt gacgtgcggg tgatagggaa
     2281 acctcagctc ctgcgcgagc gggccgctcc tggccgcggc ggcgagcacc tcgcacgccg
     2341 agatgccttc cgtgacgacg atgaacacca ccggtgacac cggtctgaac gtggcggtgc
     2401 cccgaaggcg cagccggaag ggtcgttgat cgctggccac cttcgccagg tgctcgtcga
     2461 ccgtcgccag gttgccgtcg atctccgtcg gtggcaccaa cgtgatgtgg gtggggatcg
     2521 catcggcttg cggatcaccg aaggctgcac gctgctgttg cagctgcgtt gcccacggct
     2581 ccggaatagc gacggcgaca ccgatcgtgg acacttctgg accctaccgc cgcccgctcg
     2641 ccgatcgtgt gccgctcatc tggccggcac gaagccgatc cggtcgtacg ccgtcgccag
     2701 cgtctcggcc gcaaccgctc ttgcccggtc ggcaccggtc cgcaagacgg cgtcgagctc
     2761 tgcgcggtcc tcgagcagct cgatggtcct tgcgcggaac ggtgtcacga ggtcgacgac
     2821 gatctcgccg aggtccttct tgaggtcgcc gtagcctctg ccgtcgtact ccgcggcgac
     2881 gtcgtcgacg ctgcggcccg acaaggcgga gtagatcgtc aagaggttgg agacgccagg
     2941 cttctcctcc tcgtcgtagt acacctcccg ccccgaatcc gtgacagccg agcggatctt
     3001 cttggcggat cggctcggct cgtcgagcag ctcgatgagg ccggccggcg acgacgccga
     3061 cttgctcatc ttggcggtgg gctcctggag gtcggtgatc ttcgcggtcc ccttgatgat
     3121 gtgcggttcg ggcagcacga acgtctcacc gaagcggcgg ttgaaccggg cgccgaggtc
     3181 acgggtgagc tcgaggtgct gccgttggtc ctcaccgaca ggaacctggt tcgcctggta
     3241 gagcaggatg tccgcggcct gcaggatcgg ataggtgaac aggccgaccg tggcttgccc
     3301 cccgcgcgcg gacttgtcct tgaactgggt catccgggac gcctcgccga agccggtgat
     3361 gcagccaagt acccaggcga gctgcgcatg ctcgggcacg tgtgactgta cgaacaacgt
     3421 gcactgggcg gggtcaaggc ccatcgcgat cagctgcgcg gccgacacga gcgtccgctc
     3481 ccgaagcagc ttcggctcct ggtccaccgt gatcgcgtgc aggtccacga tgcagtagaa
     3541 ggcgtcgtgg tcgaactgga gcgccaccca ctgtcgcaac gcacccaggt agttcccgag
     3601 atggaacgag tcggcggtcg gctggatacc ggagagcacg cgcggtcgtg tcgggacggc
     3661 aggcgtggac atgcgcgtca ttctgccaat ctcgctgctg cgacaaccga gcggcctcgc
     3721 caggtgtcgt gctcatgtca ggatcacgtc acgcaccacg tctccacgtg ggtctacgac
     3781 aaggcgctgc catggccgag cacgacttcg tccgcgaggt ctacgacgcg gcgtacccac
     3841 gtctcgtcgc gcagatgttg gtgttgtgcg gcgaccaggg cgacgccgag gacgtcgttc
     3901 aggaagcgtt cgtgaaggcg atgagccagc gttccgcctt cggtcggctc gacaaccccg
     3961 aggcgtggct ccgcacggtg gcgctcaacc tcctgcgcaa ccggtggcgc cgctcagccg
     4021 tgttccgcag gctgatgccg cgtcttcccg gatcgcagca tcaggtcgag ctctcgccgg
     4081 accgggtggc ggtgatcgag gccctgagga agctcgacca accactgcgc gaggtcgtga
     4141 cgctgcacca catcggcgac cttcccgtcg aggaggtggc ccggaccctc ggcatcccgt
     4201 cggggacggt caaggcccgc ctcgtccggg ggcgggccgt gctcgccgag cacctggccg
     4261 agacgaccgc cgaccgcgag gagaaccacc atgtctgatc cgaccgacct cgacacgcgg
     4321 ctcgccacac ttgcacatga tgtccgcggc aaggtcgtcg caccgtcgta cgacgacctg
     4381 gtctcgacct cccgcaggcg gcggcgcagc agcgctctgc tctccaccgt ggcgacgctc
     4441 gtcgtcgtcg ggctggcgtg ggcaggtgtg cacggccttc acgacgacac gagcacgccg
     4501 acgcagcccg ccaacggtcg gcacctgacg accgagccga tcccacggcc cacgcatgaa
     4561 agcccgccag ctgggagtcg acggctcacc gccgagcaga tcgtcgacgc gagcgactcg
     4621 tacctggtgg cgttcgccga gtcgggcgcc gatcctgagg cgcaggtgtc ggtgtggcgc
     4681 tgtcgggaac agccgaactg cccaggctgg cgcggcggca tcgcggtgac cgacgacgac
     4741 tttggcaccc ggacagtgat cgaccttcct cgcaacgcga acccggtcgc gcaggacgtg
     4801 ggcgacaaga tgttcctcgt cggcgatgca ccgaacggtg tgctcctgcg ccctgacggc
     4861 acgaccgtcg cgatgacgct gtaccgcacc acggggccac tcgcgtccgg ggaggtcctg
     4921 gtgcgcgtcc ataccggtcc ggcgacgttc ggtggcctgg acccgcagac cggacgggtc
     4981 cacctgctgg cggttccgcg gtcggacggc gtggacctgc aaggcaacgg cagttggttg
     5041 tggggcgtgg cgttcacgga caccgctgac tcggtcatgc aacgggtcat ctggagcgat
     5101 gacggtggca cgacgtggaa cacgaagtgg aacacgcgcg tcacccgctt ggatgcgaac
     5161 cggtttctgt cgccgctccc cagtcagcga gtcggaacca tgccgttcct ggtgacgagc
     5221 gacggtgcca cgttgactcc gtacgggggc gtgtacggca gccaggacgg cggccagacg
     5281 tggcagtcgt accccggatg ggccgatccg accggatacg tcggtaccgg gctgatccag
     5341 ccggacggtt cgctgctgtt gaacgtgatc gactggagcg acgccggccc gcacaagccg
     5401 agccgtcaac cgatcggtct gtatgcgagc tctggaacgg actgggggac gttgcagttc
     5461 gtccaggatc ttccgagcac gccgggctcg accgacctgc gcgcgatgta cgccgtgctc
     5521 acctacgtgc ccgacggcca gcaggcacga gtctggatct acggcaacgg ccacgtcttc
     5581 gagtccggca gcggcatcga cacctggcgc gagatccccg tccgctgacc cggcttccgt
     5641 cgaaggtttg cgatgtttac agcccaaaag gtgtgtaaac atcgcaaacc ttcgatgggc
     5701 acagtaggtc tcggatgtga acaccgcaaa ccgtggcggg cggccctgcg gatacccgac
     5761 aaaaccggag ttcgacacca ggcaaggcgg cggagagccg gcgaccagct cacgggcacg
     5821 aacgtccggc tcttcgctgc cgagccacct cggccgcggc cgacgcggcg ccgaccgacc
     5881 gcggccacca tcgcgacagt ctccgcccgc agagacgccc tgacccagct gctaggcaaa
     5941 ctcagcgggc gaggctgtgg cgggcggcgc ggtcgacttg accttgaccc gggagacgcg
     6001 ccgaccgtcc agctcgacga ccgtgagggt gtgcccgtcg aacgaggtcg tctcccctac
     6061 cgtgggcagg tgaccgagct gctgcacgat gtagccggcc acggtctcgt acggtccgtc
     6121 cggcagcgtc aggccggttt cgtcctcgaa gtcgtcgagg ttcagcagac cgtcgacctc
     6181 gagctctccg ccaacgaggc gagtcgtctc aggctcgtcg acgtcgtact cgtccttgat
     6241 gtcgccgacc agctcctcga cgaggtcctc gagcgtgatg atgcccgccg tgccgccgta
     6301 ctcgtcggcc acgatcgcga cgtgcgcgcc ctcgcgtcgc atgtccgaca gtgccgacag
     6361 gatctggcga gtggcaggaa gcaacaggac gtcgcgggcg atctcccgca ctctgaccga
     6421 gcgcctcgac agctcccggt tgaacaggtc acggacgtgg acgaagccga tgacgtcgtc
     6481 ggaggagtcg ccgatgacgg ggtagcgcga gtgtgggcgc tccagggcga actgagcagc
     6541 cttgtacgcc ggcatcgcgg ccgggatgaa atcgacttcg gtacgcggca ccatgacttc
     6601 tcggatctgt cggtctccgg cctcgaacac ctcctcgacg atctgccgct cctcggcacc
     6661 gagggtctcc gaaccggaca ccagctcccg gatctccgca tcggtgatct gctcccgctg
     6721 ggcggtcggg tctccgccga cgagccgcac cacgaggttg gtcgacaccg acagcagcca
     6781 gatcaccggt cgcgcgagct tggcgatccg gtcgacggta ggagctaccg tcagcgagat
     6841 cgactcggcc cgctgcaagg cgagccgctt gggagcgagc tcaccgacca ccagcgagac
     6901 gtacgagatc gccagcgtga tgagcaccag cgcagtcgtg tcggccaccc cggatgacag
     6961 gccgaggtcc tcgagcttcg gcgagagcct gctcgccaag gtcacgccgc cgaacgcagc
     7021 ggagaggaaa ccggcgagcg tcacgcccac ctggacggcg gcaaggaacc ggttgggatc
     7081 agcgttgagc ttggcgacga tcgcaccgcg tctgccgtcc cgttcctcga gcgccttggc
     7141 ctggctgtcg cgcagcgaga ccagggccat ctcggcgagg acgaagaagc tgctgatcag
     7201 gatgaaaagc agcaccaggg cgatgtcgcg gagcgtgccg ctcacgacgc ctcctcagtc
     7261 ctctcgagag gtctgtgctt gtcgcggcga cacccaagac tgcactcagg actacaggac
     7321 tcgtccatca gactctctag cctacgggtc cgcttcccgg cttgcgacga cccgagccca
     7381 aatgtgcttg agcgcgctcg ctgcccagcg cagggtggac ggatggagtt tcccgatgat
     7441 gtgcccgtgc tcaccaacgg ggacgtgacg ctccgcgccc accgaaccga ggacgtcgaa
     7501 ggcgtgttcg agcaatgcac cgacccggtg tccgtgcgat ggacgaccgt cccgttggac
     7561 tacaccgccg agatggcccg cgagttcgtc accgcccgca tccccgcgat gtgggcggac
     7621 gacacggagt ggctcttcgc gatcgaggcg acgcatcacg acggccaccg ccgcttcggc
     7681 ggcaccctct ccctgcgcgc cgaaggccac cggcgggccg agatcgcatt cggggcacac
     7741 cccggcgtgc gcgggcatgg cgtgatgacc actgcggtga acctcctgtt ggactggggc
     7801 ttccacgaac gagatctgga aacgatcatc tggctcgccg agcgcgggaa cttcgcgtcc
     7861 cggcgggtgg cctggaagtc ggggttcacg ttcggcggcg tactcccgaa gtggctcgac
     7921 cacaggggca cgctcgtcga cgcgtggacc ggcacgctgc atcgcaccga tgctcgggag
     7981 ccgacacaca cgtggtacga cgtccctgtg ctgaccggtg agtccgtcgt cgtacgaccg
     8041 cagcgtctcg acgatgccga ccggatcgtg gaagcatgct ccgacgagat cacgcagtat
     8101 tgggtgccct ccctgccgtc cccgttcaca cgcgatgacg ccgtcgactc catcacgtcc
     8161 ggggcagagg cggcggcaca gggcaaccac ctccagtggg ccgtcgccga tcgtcatacc
     8221 gaccaactcg tcgcggtggt ggggctgccg cggatcaagc gtgggaacgc cgaagtcggc
     8281 tactggaccc atcccgcggc gcgtggacgg ggcgtgatga aggacgcgct cggccagctc
     8341 atccgccatg ccttcaccgc ggtggagcgt ggtggactcg gcctgcaccg gctgtacgtc
     8401 aggactggtg tcgccaacgt cgcctcgcaa cgcgttgcga ccgcgaacgg attcgttccg
     8461 cgcggcaccg agcggcaggt cgagcagctc cgcgacggga cgtatacgga tttcgccttc
     8521 ctcgatctgt tgcgcgacga gtgggagggc tagcgcgatg cggtatcccg acgacgtacc
     8581 cgtgctcacc gagggcacgg tgaccttgcg cgcccatcgc gaggacgacc tcggcgggat
     8641 gtacgagcag tgcatcgacc cggtcagtca acggttcacg agcgtcccgg tgccgtatac
     8701 gcgggccgag gctcagacat tcctcgacag tcgccgcacc gcctgggaga aggatcaaca
     8761 ctggagcttc gcgatcgagt ccgagtgggg agtcggaccc agcggtttca gcggctcgat
     8821 cggactgcgt caccacgcgc caggtcttgc ggagatcggc ttcggcgccc acccgacggc
     8881 acgcggccac ggtgtgacga cacgcgcggt caagctcttg gccgactggg cgttcgggga
     8941 gcaagggatc cggaccatcc gttggcaagc acaggtcggc aacaccgcat cgcgccgggt
     9001 ggcctggaag accggcttca cattcgaggg cgccagccgc agcagcctgg tccagcgcgg
     9061 cgtgctccac gacgcatggc gcgcgacgtt gctggcgacg gacacccgcg aacccaagac
     9121 ccgttggctc gacaccccaa cactcgacgg cgaccgggtc aggctgcgcc cgctgcgccc
     9181 cgacgacgtg cagcgttacc tcgagacgaa caacgacccg gagacccttg tctggctcgg
     9241 cacgatcccg ttcccccacg acagcgaacg cttccaccgc cacctcgccc gcgccagcac
     9301 agggccgtcc gagggagcca gtgtcgagtg ggcgatggcc gaggtcggtc gggacgagta
     9361 cgtcggcacg gtcacgctct tcggcatgac cggcctcgac tacaagtctg ccgaggtcgg
     9421 ctaccgtacc catcccgact cccgtggcaa gggattcctg aaggaggcgc tgcggttggc
     9481 catcgggcat gccttcactc ctggggagga tggtggcatc ggtctcgaac gcatctcctt
     9541 gaacgccggt gccgacaacg agggttcgct gcgggccgcg cgggcctgcg gcttcaccga
     9601 gaccggccgc gaccggcagt gctacgacct gtacgacggc tcggtggtcg acctgatccg
     9661 gttcgacctg ctccgctcgg agtacgccgg ctgaccgtcg tatgctgcgc cgatggtgac
     9721 gacgtggcgg gctccgggcc gggtcaacct catcggggag cacgtcgact acgccgacgg
     9781 actgtgtctg ccgttcgcac tgccgctgac gacgacggcc acggtgtcgg cccgtccagg
     9841 tcacgacgtg acggtcacct ccgcgggcaa gacggagacg ttcccggtcg aatgcgggcc
     9901 gggcgacgtg gacggctggg cggcgtacat cgccggtgtg atctgggcgc tccggcagga
     9961 cggcgcagag ctgccgggcg tccacatcga agttgcaagc gacgtgccgg tcggtgccgg
    10021 cctctcctcg tcggcggcgc tcgaatgtgc ggttgccggg gcgatcgccg acgaggcggg
    10081 tctggagatc gctccggtag acctcgccgc tcgagcacgg cgcgcggaga acgacttcgt
    10141 cggcgtaccc agcggcccca tggaccagct ggcctcggtg ctcgggtccg acggacacgc
    10201 cgtcctgatc gactgtcgcg acctgtcgca ccggctcgtg ccgttcgatc ccgaggccga
    10261 cgggttgcga ctcctggtca tcgacaccca tgcgcaccac acgcacgccg gcggagagta
    10321 cgccgagcgc cgggagcagt ccgagagcgc gcgacgcatg ctgagcctct cgtcgctacg
    10381 cgaagccggg ctcgacgatc tcgacggcat cgacgctccg gtcatacgcc gtcgcgcgca
    10441 ccacatcgtc accgagatcg cccgggtgag cgacgtcgtg gagatcctgg acgccgggca
    10501 gccgcaggac atcggtcctg ccctcaccgc ctcgcacgag tcgctgcggg acgacttcga
    10561 ggtgtcgtgc gacgagctcg acgccgcagt cgacgccgcc ctgaccgccg gcgcactggg
    10621 tgcgcggatg accggtggcg ggttcggcgg ctgcgccatc gcactcgtcc gcgaaaccga
    10681 cgaggccgtg gtcaccgagc atgtcgtgag cacctacgcg agccgcggct ggacacgtcc
    10741 cacgatcttc cccgcgcgcc catccgccgg tgcccaccgc gtctgaccga cttgtcagaa
    10801 gctcataggc ccatagctct actgacttct gacaactcgg ctaccaggac gtgggacggg
    10861 gacggccctc ctcgtagccg gcggcactct gcaccccgac cacggcacgt tcgtggaact
    10921 cctcgatcgt cgacgcaccg gcgtacgtga acgcactgcg gacgccggcg acgatcatgt
    10981 cgatcaggtc ttcgacaccg gggcgggccg ggtcgaggta catcctcgac gacgagatgc
    11041 cctcctcgaa cagcgcggac cgggcacggt cgaaggcgga ctgctccctg gtgcgctgcc
    11101 gaacggctcg cgccgacgcc atcccgaagc tcaccttgta gagccgtccg tcggcgtcgc
    11161 tggcgagatc tccggcactc tcgtaggtgc cggcgaacca cgagccgatc atcacgctcg
    11221 atgcgccggc ggccagtccc agcgccacgt cgcggggata cttcactccg ccgtcagccc
    11281 acacggtcgc accggagtcg gccgcagcac tggcacagtc gaggaccgcc gagaactgcg
    11341 ggcggccgac gcctgtcatc attcgcgtgg tgcacatggc gcccgggccg acaccgacct
    11401 tgacgacgtc ggcaccggcc ttgaccagct cgtgggctcc tgccgctgac acgacgttcc
    11461 cggccacgac cggcgtacgc cgaccggtct cggcctcgta ccggtcacgg gcggcgacga
    11521 ccagcggaag cacc
//
LOCUS       DATBFZ010000137         7550 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_5624695, whole genome shotgun sequence.
ACCESSION   DATBFZ010000137 DATBFZ010000000
VERSION     DATBFZ010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 7550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7550
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5624695"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(131..934)
                     /locus_tag="VH419_04105"
     CDS             complement(131..934)
                     /locus_tag="VH419_04105"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY7042834.1"
                     /translation="MATQPETFQIPLEAAELYEERFVPSLFAEWAPHLVDAAGVSPGQ
                     SVLDVACGTGIVARTAAARTTPGGRVVGVDLNEAMLMVARRVAPSLDWRRGDAADLPC
                     GEGEFDAVLCQMALMFFPNRPAAMREMTRVTMAAGVVAVCVPAALDAQPAYGRFVDMV
                     ADRAGPDAASLLHTYWSCGRLDALHALFDAAGLVVTQSDTRTSTASFPSVDAFVATEV
                     ESTPLVQRLSTQQYDTIKLDAREVLVEYVKRDGLAVPLVGHIVVGTPRG"
     gene            complement(1069..2739)
                     /locus_tag="VH419_04110"
     CDS             complement(1069..2739)
                     /locus_tag="VH419_04110"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEY7042835.1"
                     /translation="MERESAGQVLTRGEAALAAGQWSDAKEAFDAAIATGESAQAWLG
                     LAEAAWWLGAPQASVDACTRAYRLFRQDSDAAGAVRCAVWLAITYKANFGNVVAANGW
                     LSRADRLLEALEVGPLHAWVLIARAYRNTDLPTAKELTARAVQIARDCGNIDAEIVGL
                     SQLGLIEVSAGDPAGGFALIDEAMTAALSGEVSELDTVVYVCCDMLNACDVDADEDRA
                     AKWCRVADDFIATYGCPFLYGECRTLYGGLLVATGGWQQAEQELDAALRITADTAPEV
                     RRRALTRLARLRIRQGRLDEAEQVLSDLDRRLVDDAEFALARAALRLASGDDAGVCRL
                     LEQRLDRWSRHRARLAEALELLVPAQLAAGDQAGAAGSADRLAALVVDGRSDRVEAAA
                     ETAAGMVAAAGGDASKAATLLETAVTKWAVLGLPFEAASCLLELAKVWAAQSPERAGD
                     HARRALVTFEELGAGQLRNEAARFLRSIGCSVPPGPRSAGALTKRELEVLELVAAGLS
                     NPEIARRLYVSSRTVSHHVSNILTKLQLRNRAEAAAYAQRTVQSGPAG"
     gene            complement(2771..3340)
                     /locus_tag="VH419_04115"
     CDS             complement(2771..3340)
                     /locus_tag="VH419_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007078121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042836.1"
                     /translation="MKLFRNVFGRSQPPAANLDNLFAVPSAAITLEAATGFKPTGVAS
                     VCYRQAEGGAFASTQQDLQELLDADAGPKVERTTDEFGYTWLLCRGDPGELADLVTDV
                     HAVNASLENAGFGPSLLCSLVGFADGDGRRFALVYLYKQGTFYPFAPAGDKQRDNALE
                     LQIRGALENEMPIEPELSRWMAVFNAPGL"
     gene            complement(3433..4332)
                     /gene="htpX"
                     /locus_tag="VH419_04120"
     CDS             complement(3433..4332)
                     /gene="htpX"
                     /locus_tag="VH419_04120"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEY7042837.1"
                     /translation="MAKTRFRTDAGLTARMSLVMLLVGGLYVTVMAVLIAAGASFLTV
                     VFIAVALGLGQWYFSDKLAMAAMRGREVSPNEAPELHGMIDRLCAMADMPKPRVAIAD
                     IDVPNAFATGRSPNHSVVCVTTGILRRLDREELEGVLSHELSHVAHRDVLVMTFAGFL
                     GVAAGLVMRWGFFFGGGGRDRDSNNLPFIVIWLGSIVVYFVSFLLTRVLSRYRELSAD
                     RAGAYLTGKPSALASALTKISGEMARIPTRDLRETQAFNAFMFAPALNRESVSNLFST
                     HPSLEQRLEQLARISADLSRPAS"
     gene            complement(4538..4816)
                     /locus_tag="VH419_04125"
     CDS             complement(4538..4816)
                     /locus_tag="VH419_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017933954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042838.1"
                     /translation="MIIRILSEGQYDVPDDDLDALNELDSELEAAVESGDEATFGSAL
                     GALLDRVRSSGEDVAVDALVPSDLVLPHAGATIAEVRDLLSDEGLIPG"
     gene            complement(4813..5565)
                     /locus_tag="VH419_04130"
     CDS             complement(4813..5565)
                     /locus_tag="VH419_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015069948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HEY7042839.1"
                     /translation="MAGIMQRISLIFKSKANKALDKAEDPRETLDYSYTKQQELLQKV
                     RRGLADVATSRKRLELQMQQLDQQSAKLQNQAQQALSQGREDLAREALTRRSGLQQQI
                     ADLQTQHANLQAEEEKLTTASQRLQAKIESFRTRKETLKATYTAAEAQTKINEAFTGI
                     SEEMGDVGMAIERAEDKTAQMQARAGAVDELIAAGALEDQVSGGKDDITRELEQMAAS
                     GDVEAQLAQMKAQIGSTPAPAQIEGQTSEEKA"
     gene            5805..6380
                     /locus_tag="VH419_04135"
     CDS             5805..6380
                     /locus_tag="VH419_04135"
                     /inference="COORDINATES: protein motif:HMM:NF022686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3043 domain-containing protein"
                     /protein_id="HEY7042840.1"
                     /translation="MFGRRTTKDASATTEVADKVGGKGRPTPTRKEAEAARKKRNAPP
                     RTRKEAAALQRQRTKENRQRQRAAMDGSGSERDLPARDRGPVKAFVRDYVDSRFTVGQ
                     FLLPFMFVMFALVYVRAAWAQNLSSSLFLTAILVLVLDSVRISRGVKKAITARFSRDE
                     ASGHTMYALLRAWQMRRLRLPKPRVKVGDHI"
     gene            6461..7459
                     /locus_tag="VH419_04140"
     CDS             6461..7459
                     /locus_tag="VH419_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013161228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family protein"
                     /protein_id="HEY7042841.1"
                     /translation="MEFRHLGTSGLKVSEISYGNWLTHGSQVEEEQARACVRQALDDG
                     ITTFDTADVYANTKAESVLGRALEGQRRESLEICTKVFWPTGPGGPNDTGLSRKHIHE
                     SINGSLRRLKTEYVDVYQAHRYDVDTPLEETMQAFADVVRAGKALYIGVSEWTAEQIR
                     TAHGLAKELGVPFVSNQPQYNMLWRVIEAEVIPTSLELGIGQIVWSPIAQGLLTGKYR
                     PGEPPPAGSRGAHEEVGAFVRRRMTDEMLERIQQLRPIADDLGLSMAQLAIAWVLANP
                     AVSSAIIGATRPEQVTENVKASGQKLDADVLAAIDDALGPEIQWDASLTQTPASRG"
BASE COUNT         1188 a         2617 c         2533 g         1212 t
ORIGIN      
        1 cccgagccgg cgacccgcgg cgttgtcgtc ggtcgacggg ggcttcgccc cgcctccctc
       61 ctccgccttg cggctcatcc acctcgaccc cgctcgcgac gagcggattc ttcaccaccc
      121 tcctgacgga tcagccgcgg ggtgtgccga ccacgatgtg tccgacgagt ggaacagcca
      181 gcccgtcccg cttgacgtac tcgacgagca cctctcgcgc gtcgagcttg atcgtgtcgt
      241 actgctgcgt cgacaggcgc tgcaccagcg gcgtgctctc cacctcagtg gcgacgaacg
      301 cgtcgacgga cgggaagctg gccgtgctgg tgcgggtgtc actctgcgtc acgaccaggc
      361 ctgcagcgtc gaagagcgcg tgcagggcgt cgagccggcc acacgaccag taggtgtgca
      421 gcagcgacgc ggcatcgggt ccggcccggt cggcgaccat gtcgacgaac cggccgtacg
      481 ccggctgcgc atccagggcg gcaggcacgc acaccgcaac cactccagcc gccatcgtga
      541 cgcgcgtcat ctcgcgcatg gcagctgggc ggttcgggaa gaacatcagc gccatctgac
      601 acagcaccgc gtcgaactcg ccctcaccgc agggcaggtc ggccgcatca ccacgtcgcc
      661 agtcgagcga gggcgcgaca cgtcgtgcga ccatcagcat ggcctcgttg aggtcgacac
      721 cgacaacccg tccgcccggc gtcgtacgcg ctgcggcggt gcgcgcgacg atgccggtgc
      781 cgcaggcgac gtccagcacc gattgccccg gcgagacacc ggccgcgtcg acgaggtggg
      841 gagcccattc ggcgaacagc gaggggacga accgctcctc gtagagctcg gccgcttcga
      901 gcgggatctg aaaggtctct ggttgggttg ccatggtcag cagcatcgac ctggcgccgc
      961 ccggtttcat cggtcaatcg gcccatgttc ctcaccggcc gctcgccacg ctcgccacgc
     1021 tcgcgacacg gcgacccggc gacccggcga taccactggt gcgacggatc acccagcagg
     1081 gccggactgc acggttcgct gagcataggc cgcagcctcg gcccggttgc gaagctgcag
     1141 cttggtgagg atgttgctga cgtggtgcga caccgtccgg gagctcacgt agagcctccg
     1201 ggcgatctct gggttcgaga gccctgctgc caccagctcg agcacttcga gctcgcgctt
     1261 cgtcaacgca ccagcggatc gtggcccggg gggaaccgag catccgatcg agcgcaggaa
     1321 gcgggctgcc tcgttccgga gctggcccgc acccagctcc tcgaacgtca cgagagcccg
     1381 ccgggcatgg tcaccagctc gctcgggaga ctgcgctgcc cacaccttcg cgagctcgag
     1441 cagacagctg gcggcctcga acggcagtcc gagcactgcc cacttcgtca cggccgtctc
     1501 cagcaacgtt gccgccttcg aggcgtcccc tcctgccgcc gccaccatcc cggcggccgt
     1561 ttccgcagcc gcctccaccc ggtcgcttcg tccgtcgaca acgagcgccg ccagccggtc
     1621 tgcactgcct gcggcacccg cctggtctcc ggccgcgagc tgcgccggca cgagcagctc
     1681 gagcgcctca gccagtcgcg cccggtgacg agaccaccgg tcgagccgtt gctcgagtag
     1741 gcggcagact ccggcatcgt cgccggatgc cagccgcagc gcggctcgag ccaaggcgaa
     1801 ctccgcatcg tcgaccagtc gccgatccag gtccgacagg acctgttcag cctcgtcgag
     1861 acgaccctgg cgaatgcgca gtcgtgccag ccgggtgagc gcgcggcgcc gcacctctgg
     1921 agccgtgtcc gcggtgatgc gcaacgctgc atccagttcc tgttcggctt gttgccaccc
     1981 gcctgtcgcg acgagcaggc cgccgtacag cgtccgacac tcgccataca ggaacgggca
     2041 gccgtacgtc gcgatgaagt cgtctgcgac ccgacaccac ttggccgccc ggtcctcgtc
     2101 ggcgtcgacg tcgcaggcgt tgagcatgtc gcagcagacg tagaccacgg tgtcgagttc
     2161 ggacacttcg ccgctcaacg ctgccgtcat cgcctcgtcg atgagggcga agccaccggc
     2221 gggatcgcct gccgagacct cgatcaggcc gagctgggac aacccgacga tctcggcgtc
     2281 gatgttgccg cagtcgcgcg cgatctgtac cgctcgggcc gtgagctcct tggcggttgg
     2341 gaggtcggta ttgcggtatg cgcgtgcgat caacacccag gcgtgcagcg ggccgacctc
     2401 gagcgcttcg agcaaccggt ctgcgcgcga cagccatccg ttcgccgcaa cgacgttccc
     2461 gaagttcgcc ttgtaggtga tcgccaacca aacggcgcat cggactgcgc cggcggcgtc
     2521 ggagtcctgt ctgaagagcc ggtaggcgcg tgtgcatgcg tccacgctcg cctgcggggc
     2581 gccgagccac caggcggcct cggccaggcc gagccacgcc tgcgccgact cgcccgtcgc
     2641 aatggcggcg tcgaacgcct ccttcgcgtc ggaccactgg ccggcagcca gcgccgcctc
     2701 acctcgcgtg aggacctggc cggcggactc ccgctccatc accacatctt ggcaacgtga
     2761 cggctgcccg tcagagaccg ggggcgttga agacggccat ccatcgggag agctcgggct
     2821 cgatcggcat ctcgttctcg agcgcgccgc ggatctggag ctcgagcgcg ttgtcacgtt
     2881 gcttgtcgcc tgccggtgcg aacgggtaga acgtgccctg cttgtagagg tagaccagag
     2941 cgaaccgtcg accgtctccg tccgcgaagc cgaccagcga gcagagcagg gaggggccga
     3001 agccggcgtt ctcgagcgag gcgttgacgg cgtgcacatc ggtgacgagg tcggcaagct
     3061 caccggggtc gccccggcag agcaaccagg tgtaaccgaa ctcgtcagtt gtccgctcca
     3121 ccttcgggcc cgcgtcggcg tccaacagct cctggaggtc ctgctgggtc gatgcgaagg
     3181 cgccgccttc cgcctggcgg tagcacaccg aggccactcc cgtgggtttg aacccggtcg
     3241 ctgcctcgag ggtgattgct gccgacggca ctgcgaacag gttgtcgagg ttggcggccg
     3301 gcggctggct acgaccgaag acgttgcgga acagcttcac ggctcgatcc ttccaggctc
     3361 acgtggccgg ttccgcggca ccgatgcgct ggtacgccgg tcggcacccc gccggcatac
     3421 acgctcgacg gatcagctcg cgggccggga caggtcggcg gagatgcggg ccagctgttc
     3481 cagccgctgc tcgagcgacg ggtgggtcga gaacaggttg ctcaccgact cgcggttcag
     3541 ggcgggcgcg aacatgaacg cattgaacgc ctgcgtctcg cgcaggtcgc gggtcgggat
     3601 tcgcgccatc tcaccggaga tcttggtcag cgccgacgcg agggccgacg gcttgccggt
     3661 caggtaggcg ccggcccggt cggccgacag ctctcgatac cggctgagca cgcgcgtcag
     3721 caggaacgag acgaagtaga cgacgatgct gccgagccag atcacgatga acggcaggtt
     3781 gttgctgtcg cggtcacgtc caccgccgcc gaagaagaag ccccagcgca tcaccagtcc
     3841 ggccgccaca cccaggaagc cggcgaacgt catcacgagc acgtcacgat gcgcgacgtg
     3901 tgacagctcg tgcgacagca ctccttcgag ctcctcccgg tcgagtcgcc ggaggatgcc
     3961 ggtcgtcacg cagacgacgg agtggttggg gctgcggccg gtggcgaacg cgttgggtac
     4021 gtcgatgtcg gcgatggcca cccgtggctt cggcatgtcg gccatcgcgc acaaccggtc
     4081 gatcatgccg tgcagctcag gcgcctcgtt gggcgacacc tcccggccgc gcatcgccgc
     4141 catggcgagc ttgtcggaga agtaccactg gccgaggccg agcgcgacgg cgatgaacac
     4201 gaccgtcagg aagctggcgc cggcggcgat cagcaccgcc atcacggtga cgtagagccc
     4261 cccgaccaac agcatcacga gactcatccg ggcggtcagc ccggcgtcag tgcggaaacg
     4321 agtcttggcc atgtcgggtg ctgctctccc tcggttggtg aaacgggttg cgccgtcgcc
     4381 gaactcacgt ccggacgacg tcatctagta cgacgcctgg cgcctcccgt ttgttcctga
     4441 ccggccttcc acagacgact cacgccgagg tggcacgttt cgttgctttc tggcggcgtg
     4501 tcgacctgcc atcgccacct cggcgggtgc gggggcgtca gccggggatg aggccttcgt
     4561 cggacaaaag gtcacgaacc tcggcaatgg tggcgccggc gtgcggaagg acgaggtcgg
     4621 aaggcaccag cgcatcgacc gcgacgtcct caccactgct tcggacccgg tcgagcaggg
     4681 ccccgagcgc cgagccgaac gtcgcctcgt caccggactc gaccgcagcc tccagctcgg
     4741 agtcgagctc gttcaacgcg tcgaggtcgt cgtccggaac gtcgtactgc ccctcgctca
     4801 ggatcctgat gatcatgcct tctcctccga cgtctggcct tcgatctgcg ccggggccgg
     4861 agtgctgccg atctgggcct tcatctgggc cagctgcgcc tcgacgtcgc ccgacgccgc
     4921 catctgctcc agctcccggg tgatgtcgtc cttgccgccg gagacctggt cctcgagcgc
     4981 gccggccgcg atgagctcgt cgacagcacc cgcccgggcc tgcatctgcg cggtcttgtc
     5041 ctccgcccgc tcgatcgcca taccgacgtc gcccatctcc tcggagatcc cggtgaacgc
     5101 ctcgttgatc ttcgtctgcg cctccgcggc cgtgtaggtc gccttcagcg tctccttgcg
     5161 ggtgcggaac gactcgatct tcgcctggag ccgctgcgac gccgtggtca gcttctcctc
     5221 ctcggcctgc aggttggcgt gctgggtctg caggtcggcg atctgctgct gcagaccgga
     5281 ccgccgcgtc agtgcttcgc gggcgaggtc ctcacggccc tgggacagtg cctgttgcgc
     5341 ctggttctgc agcttggcgg actgctggtc gagctgctgc atctggagct ccagccgctt
     5401 gcggctggtg gcaacgtcgg cgaggccacg gcgaaccttc tgcagcagct cctgttgctt
     5461 ggtgtagctg tagtcgagcg tctcgcgcgg gtcttcggcc ttgtcgagcg ccttgttggc
     5521 cttggacttg aagatcagcg agatgcgctg catgatgccg gccatcgtgt ggtgcctgcc
     5581 ccttctgtga cgtcggcggt aggacgcggt cggcgtacac cggccggttg aacggtcacg
     5641 ttgcggctcc cgctccgttt ggtcccaacc ctacggtgct gcgaaaatcg atgtcaccca
     5701 gcacgtcgcg gtcaccgtca acaggtaggc tcccctgcaa tcgggacatg gcgagtctgg
     5761 cgatcccggc atggtggttc acgtactgag gtaggcacgg ttcggtgttt ggtcggcgta
     5821 cgacgaagga cgcgtcagcg acaacagagg tcgcggacaa ggtcggtggc aaggggcgac
     5881 cgacgccgac ccgcaaggag gccgaggcag ctcgcaagaa gcgcaacgcc ccgccccgca
     5941 cccgcaagga agccgctgcc ctgcaacggc agcggaccaa agagaatcgt cagcgtcagc
     6001 gtgccgccat ggacggcagc ggttcggagc gtgatcttcc ggcgcgggac cggggaccgg
     6061 tgaaggcgtt cgtccgcgac tacgtcgact cccgtttcac ggttggccag ttcctgctgc
     6121 cgttcatgtt cgtcatgttc gcgctggtct acgtccgtgc ggcgtgggcg cagaacttgt
     6181 cctccagcct cttcctcacc gcgatcctgg tgctggtgct tgattcggtc cgcatcagcc
     6241 ggggcgtcaa gaaggcgatc accgcacgtt tctcgcgcga tgaagcgtcc ggccacacga
     6301 tgtacgccct gctgcgggcc tggcagatgc gccggctgcg gctgccgaag ccgcgcgtca
     6361 aggtcggcga ccacatctga cggtcggcca tcccggcgta cgtcacgccg agtcgcggac
     6421 gcaaggttgt gcactccccc gtgccgcgta gggtccagac atggagttca ggcacctcgg
     6481 aacgtcaggt ctcaaggtca gcgagatcag ctacggcaac tggctgacgc acggctcgca
     6541 ggtcgaggag gaacaggcgc gcgcctgcgt acgtcaggcc ctcgacgacg gcatcacgac
     6601 cttcgacacc gccgacgtct acgcgaacac gaaggcggag tccgtgctcg gccgtgccct
     6661 ggaagggcaa cgacgagagt cgctggagat ctgcacgaag gtgttctggc cgactgggcc
     6721 tggcggtccg aatgacacgg gactgtcacg caagcacatc cacgagtcca tcaacggcag
     6781 tctgcgccgg ctgaagacgg agtacgtcga cgtgtaccaa gcgcaccggt acgacgtgga
     6841 caccccgctc gaggagacca tgcaggcgtt cgcggatgtg gtccgggcag gcaaggcgtt
     6901 gtacatcggt gtctcggagt ggacggcaga acagatccgg accgcgcacg gactggcgaa
     6961 ggagcttggt gtcccgttcg tctccaacca gccgcagtac aacatgctct ggcgggtcat
     7021 cgaggccgag gtgatcccga ccagcctcga gctcgggatc ggccagatcg tctggtcccc
     7081 gatcgcccag ggcctgctca ccgggaagta ccgaccaggc gagccgccac cggccggttc
     7141 gcggggtgcc cacgaggagg tcggggcgtt cgtacgccgc cggatgaccg acgagatgct
     7201 ggagcggatc cagcagctgc ggccgatcgc ggacgacctc ggtctctcga tggcgcagct
     7261 cgcgatcgcc tgggtcctcg ccaacccggc cgtgtcgtcg gcgatcatcg gcgccacccg
     7321 tcccgagcag gtcaccgaga acgtcaaggc gtccggtcag aagctggacg ccgacgtgct
     7381 cgccgccatc gacgacgcac tcgggccgga gatccagtgg gacgcttcgc tgacccagac
     7441 gcccgccagc cgcggctgag gccactgggg gcggtcccgt ccgcggctac tccgcggaca
     7501 acgacatcgg gccgtagacg acgtggtcgt cgtccagcag cgtcgcctga
//
LOCUS       DATBFZ010000138         5552 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_3041977, whole genome shotgun sequence.
ACCESSION   DATBFZ010000138 DATBFZ010000000
VERSION     DATBFZ010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 5552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5552
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3041977"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(263..3220)
                     /locus_tag="VH419_04145"
     CDS             complement(263..3220)
                     /locus_tag="VH419_04145"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HEY7042842.1"
                     /translation="MSALHQRRLVLAAVLPLTATAALVGLPAIQANAVSTTIVIHEVY
                     GGGGNTGAPLDHDFIELTNLGSSTVDVSDWSVQYASATGTTYTVTNLTGQVPAGETYL
                     VQEASGANGGPLPAPDATGSINLSGTNGKVALVSNQTALACGADCDQAAGVVDFIGYG
                     TANDFETAAAPAGSNTTSLARGGADSDNNAADFTAGSPSPVGCGDPCLPPSQPATIAQ
                     IQGAGHLSPLNGDSVTDVAGVITARASNGFYMQDPDGSDIDGASSGILVFTGAAGLTD
                     AMIPGTAVTVDGSVTEFRPGSTGLTVTELGTPTVHVGAAGQPLPAATLVGPGGLSVPA
                     TVIDDDAAPGTQINVETQGTYQPDDDGIDFWESLEGMRVELDDARVVGPTNVAFGETP
                     IVPAGSGIETERGGIVLRQSDPNPERIVLDNSLGTTVPKANVGDTYAGPTVGVLSYDF
                     NNFHLLATQRPTLSSGGITREVATASTHDELSIATFNVENLDPTDPQSKFDDLAEIIV
                     HNLAAPDLLALEEVQDNDGPTDSGTTDADLTLQQLVDAISAAGGPAYTWEQINPRNDQ
                     EGGEPGGNIRVAFLIQQGTPLSFVERDPGTSSEDTDVTAGPDGAELTHSPGRVDPDNA
                     AWVATRVPLAGEFMFGTQKVFVVANHWSSKGGDQPLFGPNQPPLQASETKRVTQAETV
                     NSFVHEITAADADANVVVLGDLNDFEYSDSVTALTEDGALLDDLPATLPDAERYTYDF
                     EGNSQVLDHILLSSGASTQGFDYDVVHVNAEFNDQASDHDPQVVDLHLGLPAADATLA
                     ATPSTIVFGQSSTIHGVLTDHATGDPIAGATVELQSRGTAADPWAHNQNDVTAADGSY
                     SFSVQANRRTSYRVVWGGNTQFASTQSQSVVVKVAPRVTLEVSDRTVKKGTTVLFTGI
                     VAPNHAGQRVSVQHRRNGSWVTVATPKLDQDSLYRFRWTASPKGASVWRVVKPADGDH
                     VQGVSRTVSVTVH"
     gene            3389..4471
                     /locus_tag="VH419_04150"
     CDS             3389..4471
                     /locus_tag="VH419_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase domain-containing protein"
                     /protein_id="HEY7042843.1"
                     /translation="MAKSSAVMLQVGEREVRVSSPDRIVYEATELTAPITKLQVCEYF
                     AGVGHVLMRAIGERPTAMERWPDGWREGMRLATGAQDRDGDGFYQKRLPKGAPDFVES
                     VKITFPSGRPADELCPTEAASLVWAAQMGALTFHPWPVRRPDVDHPDELRIDLDPQPG
                     TSFSDAQRVAEVTRELLEEHGLRGYPKTSGNRGVHIYVHIQPKWTFDEVRHAAIGLGR
                     ELERRDGGVTTAWWKEERGSRLFLDYNQNLRDRTIASAWSLRARPGAPVSTPMSWARL
                     AEVKDPRAFNLTTIRDYLSDGDPWADMDAEAHSLEPLLHLWDSLPGGELNWPPDYPKQ
                     PGEPPRVQPSKKVAEHWDEDGNRIAP"
     gene            4510..>5552
                     /locus_tag="VH419_04155"
     CDS             4510..>5552
                     /locus_tag="VH419_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017933052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY7042844.1"
                     /translation="MRSRWWTLTAVCTGVFMLLLDITIVNVALPDIQSAFGASLSDLQ
                     WIIDAYALTLAALLLTAGSLADLYGRRKLFVIGVAIFTLGSLACGLAQSIEFLAISRA
                     GQGVGGAIMFATALALLSSAYRGRDRGIAFGAFGATTGVAVAVGPVVGGILTSGLSWR
                     WIFLVNVPICFFAIATAVLRVDESRDPGAGRPDWLGFVTFSAGLGLLVLGLIRSGVDG
                     WDAPLVEWSLVASAVLLVVFVITQVVQRHPMFELSLLRKPTFVGGLLAAFGVSASIFS
                     LLTYLVIYVQNILGYSAIGAGLRFLCLSGTAFIAAAVAGRLTSFVSTKWLIAPGFFVL
                     GLGTVLMLGVQVTS"
BASE COUNT          764 a         1810 c         1970 g         1008 t
ORIGIN      
        1 accagggccg ctcggtgcca gccttgcgga ggacggcgta ctcctccggg ctcagctgag
       61 cccgccactc ctcctcggtc ttctcgacgc ggtcacggtc ggtcgggctg tctgcggcgg
      121 tcgacgtctg ggtgggctcc atgactccag cctacgaggt ggcccaaccc gacccctgtc
      181 cgctgtggtc gccgcgaatt gcgggaaacg tccggcgcga acggtgttcg gagcatgcac
      241 gttcccgcaa ttcgcgggag cctcagtgga cggtcaccga gacggtccgg gagacgcctt
      301 ggacgtggtc gccgtcggcc ggcttgacga cacgccacac cgacgccccc ttcggcgagg
      361 ctgtccagcg gaaccggtac aggctgtcct ggtccagctt cggcgtcgcc acggtgaccc
      421 agctgccgtt gcgtcgatgc tgcaccgaca cccgctgccc ggcatggttg ggggcgacga
      481 tcccggtgaa cagcaccgtc gttcccttct tgaccgtccg gtcgctgacc tcgagcgtca
      541 cccgtggtgc caccttgacg acgacggact ggctctgggt cgaggcgaac tgggtgttcc
      601 caccccagac gacccggtag ctggtgcgtc ggttcgcctg gaccgagaac gagtacgagc
      661 cgtccgccgc cgtcacgtcg ttctggttgt gcgcccacgg gtcggcagcc gttcctcggg
      721 actggagctc caccgtcgcg ccggcgatgg ggtcgccggt ggcgtggtcg gtcaacacgc
      781 catgaatggt cgacgactgg ccgaacacga tcgtcgaggg cgtggccgcg agggtggcgt
      841 cggcggcagg caacccgagg tgcaggtcga cgacctgcgg gtcgtggtcg ctcgcctggt
      901 cgttgaactc cgcgttgaca tgcacgacgt cgtagtcgaa gccctgcgtg ctcgcgcccg
      961 agctcagcag gatgtggtcc agaacctgcg agttcccctc gaagtcgtag gtgtagcgct
     1021 cggcgtcagg cagcgttgcc ggcaggtcgt cgagcagcgc gccgtcctcc gtcaacgccg
     1081 tgacgctgtc ggagtactcg aagtcgttga ggtcgccgag tacgacgacg ttggcgtccg
     1141 cgtcggcggc ggtgatctcg tggacgaagc tgttgacggt ctccgcctgg gtgacgcgct
     1201 tggtctccga cgcctgtagg ggcggctggt tcgggccgaa cagcggctgg tcgccgccct
     1261 tgctgctcca gtggttcgcg accacgaaca ccttctgggt gccgaacatg aactcacccg
     1321 ccagcggcac ccgcgtcgcg acccatgccg cgttgtcggg gtcgacacga ccgggggagt
     1381 gcgtcagttc ggcgccgtcc ggtccggccg tgacgtcggt gtcctcactc gacgtgcccg
     1441 ggtcacgctc gacgaacgac agcggcgtgc cctgctggat caggaacgcg acccggatgt
     1501 tgccaccggg ctcgccgcct tcctggtcgt tgcgcgggtt gatctgctcc caggtgtacg
     1561 ccggaccgcc ggcggcgctg atcgcgtcca cgagctgctg cagcgtcagg tcggcgtcgg
     1621 tggtgccgga gtccgtcgga ccgtcgttgt cctgcacctc ctcgagtgcg agcaggtcag
     1681 gtgccgcgag gttgtgcacg atgatctcgg ccaggtcgtc gaacttcgac tgcgggtcgg
     1741 tcgggtcgag gttctccacg ttgaacgtcg cgatggacag ttcatcgtgc gtgctcgccg
     1801 tggcgacctc acgggtgatc ccgccgctgg acagtgtcgg tcgttgcgtc gccagcaggt
     1861 ggaagttgtt gaagtcgtag ctcagcacgc cgacggtcgg gccggcgtac gtgtcgccga
     1921 cgttcgcctt cgggaccgtg gtgccgaggc tgttgtcgag gacgatgcgc tccgggttcg
     1981 ggtcactctg gcgaaggacg atgccgccgc gctcggtctc gatgcccgat ccggcgggca
     2041 cgatcggagt ctcaccgaac gccacgttcg tcgggccgac cacccgcgca tcgtcgagct
     2101 cgacccgcat gccctccagc gactcccaga agtcgatgcc gtcgtcgtcg ggctggtagg
     2161 tcccctgtgt ctcgacattg atctgcgtgc cgggcgccgc gtcgtcgtcg atgacggtcg
     2221 cgggcacgct caacccacct ggcccgacca gtgtcgcggc cggcagcggc tggccggcgg
     2281 cacccacgtg gacggtcggc gtgccgagct cggtgacggt gagcccggtc gagcccggtc
     2341 ggaactcggt cacgctaccg tcgacggtca ccgccgttcc cgggatcatc gcgtcggtga
     2401 gcccggccgc gccggtgaac acgaggatgc ccgacgaggc gccgtcgatg tcactgccgt
     2461 cggggtcctg catgtagaag ccgttgctcg cgcgagcggt gatcacaccg gcgacatcgg
     2521 tgacgctgtc accgttgagc ggcgagaggt ggccggctcc ttggatctgc gcgatcgttg
     2581 ccggctgtga gggcggcagg caagggtcgc cgcagccaac ggggctcggt gatcccgcgg
     2641 tgaagtctgc tgcgttgttg tcgctgtccg cgccaccacg ggcgagcgac gtggtgttgc
     2701 tgccggccgg ggcggcggcg gtctcgaagt cgttggcggt gccgtagccg atgaagtcga
     2761 cgactccggc ggcctggtcg cagtcggccc cgcatgcgag cgccgtctgg ttgctgacga
     2821 gcgccacctt gccgttggtc ccgctcaggt tgatgctgcc ggtcgcgtca ggagcgggca
     2881 gcggaccgcc gttcgcgccg gatgcctcct ggacgaggta ggtctcgccg gcggggacct
     2941 ggccggtcag gttggtgact gtgtacgtcg taccggtcgc cgacgcgtac tggaccgacc
     3001 agtcggagac gtcgaccgtc gagctcccga ggttcgtcag ctcgatgaag tcgtggtcga
     3061 gcggtgctcc ggtgttaccg ccgccgccgt acacctcgtg gatcacgatg gtcgtggaga
     3121 cggcgttcgc ctgtatggcg ggcaggccga ccagggcagc ggtcgccgtg aggggcagta
     3181 cggcggcaag gacgagtcgt cgctggtgaa gtgcgctcac gcgagatcct tagggcatcg
     3241 aaggctggga taccgcatga ccatatgagg caacctccga ctttggggat acctggcggt
     3301 gaactttctg tggtggccac ccgtccagct tcgtggatct gccgccacgc cgagcagccg
     3361 gatgacgttg gccggataac gttggatggt ggccaagagc tcggcggtga tgctccaggt
     3421 gggggagcgc gaggtgcgcg tctccagccc tgaccgaatc gtctacgagg cgacggagct
     3481 caccgcgccg atcaccaagc ttcaggtctg cgagtacttc gcaggggtcg gccacgtgct
     3541 gatgcgggcg attggtgagc ggccgactgc catggagcga tggccggacg gctggcgcga
     3601 aggcatgcgg ctggctaccg gggcgcagga ccgtgacggt gacggtttct atcaaaagcg
     3661 gctgcccaag ggtgcgccgg acttcgtcga gagcgtgaag atcacgttcc cgagtggccg
     3721 tcccgccgat gagctgtgtc cgaccgaggc cgcttcgctg gtgtgggcgg cgcagatggg
     3781 cgccctcacg ttccacccgt ggccggtgag acggcccgac gtggatcatc ctgacgagtt
     3841 gcgtatcgac ctcgatccgc agccgggaac atcgttctcg gacgcgcagc gcgtcgccga
     3901 ggtcaccagg gagttgctcg aagaacatgg cctgcggggc tatcccaaga ccagtggaaa
     3961 ccgaggcgtc cacatctacg tgcacatcca gccgaagtgg acttttgacg aggtacggca
     4021 cgctgccatc ggactcggcc gcgagttgga gcgccgggac ggcggggtca caactgcctg
     4081 gtggaaggag gagcgcggct cgcggctctt cttggactac aaccagaacc tgcgggaccg
     4141 gaccattgcg agcgcttgga gcctgcgggc aagacctggc gcgcccgtca gcacgccgat
     4201 gtcctgggcc cggttggctg aggtcaaaga cccgcgcgcc ttcaatctca ccacgatccg
     4261 ggactacctg tccgatggcg atccgtgggc tgacatggac gccgaggctc actcgctcga
     4321 gcccttgctg cacttgtggg actcgctgcc cggaggcgag ctgaactggc cgcccgacta
     4381 tcccaagcag ccgggcgagc ccccgcgcgt acagcccagc aagaaggtcg ccgagcactg
     4441 ggacgaggac ggcaaccgga tcgcgccgtg atgtgtgcgg ggcggcccgg ggtggcaggc
     4501 tgggaagaca tgagatcgcg atggtggacg ctcaccgccg tatgcaccgg cgtgttcatg
     4561 ttgctgctcg acatcacgat cgtgaacgtc gccttgccgg acatccagtc ggccttcggc
     4621 gcatcactgt ccgacctgca gtggatcatc gacgcgtacg cgctgacgct cgccgcgctg
     4681 ctgctcaccg ccggctcgct ggcagacctc tacgggcggc ggaagctgtt cgtcatcggc
     4741 gttgcgatct tcaccctcgg ctcgttggcg tgcgggctgg cgcagagcat cgagttcctg
     4801 gcgatctccc gagccggcca gggcgtcggc ggcgcgatca tgttcgccac cgcccttgcc
     4861 ctgctgtcca gcgcctatcg cggccgtgac cggggaatcg cgttcggcgc cttcggagcg
     4921 acgaccggtg tcgcggtcgc ggtcggaccc gtggtcggcg gcatcctgac cagcggcctc
     4981 tcgtggcgat ggatcttcct cgtgaacgtc cccatctgct tcttcgcgat cgccaccgcg
     5041 gtgctgcgcg tcgacgagtc acgcgaccct ggcgccgggc ggccggactg gctcggcttc
     5101 gtcaccttca gcgccggact cggtctgctc gtgctcggtc tgatccgcag cggagtcgac
     5161 ggctgggacg caccgctggt cgagtggagc ctggtggcgt cggcggtcct gctcgtggtg
     5221 ttcgtcatca cgcaggtcgt gcagcggcat ccgatgttcg agctgtcgct gttgcgcaag
     5281 ccgacgttcg tcggcggact gttggcggcg ttcggggtgt cggcgtcgat cttctcgttg
     5341 ctcacctatc tcgtcatcta cgtgcagaac atcctcggct actccgcgat cggtgcgggc
     5401 ctgcgattcc tctgcctgtc gggtacggcg ttcatcgccg ctgccgtcgc cggccggctc
     5461 acgagcttcg tgtcgacgaa gtggctcatc gctccaggct tcttcgtcct cggtctcggg
     5521 accgtgctca tgctcggtgt ccaggtgacc tc
//
LOCUS       DATBFZ010000139         8788 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_2060193, whole genome shotgun sequence.
ACCESSION   DATBFZ010000139 DATBFZ010000000
VERSION     DATBFZ010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 8788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8788
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2060193"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1182
                     /locus_tag="VH419_04160"
     CDS             <1..1182
                     /locus_tag="VH419_04160"
                     /inference="COORDINATES: protein motif:HMM:NF019982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY7042845.1"
                     /translation="RCRIPGPTPAFPEHAFPMTPTEVRTHIRFNRYERKACRQGGSTI
                     LDHMTTADTARDMDLIRQAVGEDQLSYYGISYGTYLGATYARLFPHQVRALIVDGVLD
                     PVAWSTGRNGSGTVIPFSTRLGSGRGAADAMLSALALCDQVGQERCAFAGNARHKWRQ
                     LTNRLRKEPLEFPDGSTLAYADLIDGVLGGLYSSSDYRFLMRDLQRLWTRTNPPTKAV
                     VADDEIRAVRDRLVQRERRLPYGYGLPFFPSFEGVACSDTLNPSDPAAWQRAADRSEA
                     TQPWFGRLWTWVSAACPNWPGSAADAFRGPWHGTTANPVLLVGNAHDPATPFSGAAAL
                     HQLLDGSRLFRLDGWGHGALGESRCVTKVFDAYLVQGTLPAVGTVCEPDDLLFPVRHS
                     T"
     gene            complement(1196..1708)
                     /locus_tag="VH419_04165"
     CDS             complement(1196..1708)
                     /locus_tag="VH419_04165"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY7042846.1"
                     /translation="MASPAGALTVRRSTPDERAWVAARLVDLWGSVYAARLGVLVDAS
                     ALPALVSERAGERVGLATFDIQDDACEVVTIDAFVGGTGVGSSLLSAVVDEARAHGCR
                     RLWLITTNDNMRALRFYQRRGLRLAALHRDALALSRRLKPSIAEIGQDGIPLRDELEL
                     EMLLEPVDGA"
     gene            1775..2890
                     /locus_tag="VH419_04170"
     CDS             1775..2890
                     /locus_tag="VH419_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020386810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HEY7042847.1"
                     /translation="MSPIDVRAISAAEHLGFIQAQPRASFLQTPAWGGVKRDWKHESI
                     GWYDAGRLVGVGLVLYRQLPRIQRFLAYLPEGPVIDWASTDLGSWLSPMTAHLRAAGA
                     FGIRMGPPVVVRSWTADTVKRAMADDTHGRLGDVPPDSRNDVGGDVQATLRTLGWRPL
                     VSTGGFTAGQPQYVFQVPLAGRSEEDVLRGMNQLWRRNIKKADKAGVVVSQGKADDLA
                     AFHALYVETAERDHFTPRPLGYFQDMFAEMGQEGPDRIRLYLAHHEDDLVASTTMVRV
                     GAHAWYSYGASSTAKRDVRGSNAIQWQMMRDAMAAGADVYDLRGITDTLDPHDPHVGL
                     IQFKVGTGGDAVEYVGEWDLPLNRLLYKGFALYMGRR"
     gene            2897..3913
                     /locus_tag="VH419_04175"
     CDS             2897..3913
                     /locus_tag="VH419_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011758097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEY7042848.1"
                     /translation="MPLTLYVDGPRWRSHLRATADANRGIVPVAKGNGYGFTIGRLAR
                     RAEWLGVDMLAVGTYAEVAEVRQRFSGDVLVLEPWRPFLPTLVYDDRLIHTVGRAQDL
                     KALADIGDTPRVLLETLTSMNRHGFQLDTLAGAAATARGVHIEGHALHLPLGEGHLDE
                     VERCLATAPEKRWYVSHLTAAELTQLRTRHPGVEFRPRIGTALWLGDKQALSPRATVL
                     DARDVQRGERAGYRGRRFSKPGTLLVVSGGTTHGVALEAPTPASSARQRAVAVARGGL
                     EATGRALSPFVVDGKQRWFVEPPHMQVSLVFVPAGARAPGVGDEIDVQVRFTTTRFDA
                     IEIS"
     gene            complement(3906..4331)
                     /locus_tag="VH419_04180"
     CDS             complement(3906..4331)
                     /locus_tag="VH419_04180"
                     /inference="COORDINATES: protein motif:HMM:NF016119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class F sortase"
                     /protein_id="HEY7042849.1"
                     /translation="MLQSGRTVPVDVAVTGDDGSLRLPIDVRRAGWWNGSARIGDPLG
                     SIVVAAHVDSFTQGLGAFAELLAARPGDHLALGASRLSQEYRVVSARLVPKASLAESS
                     SLFSAAGQESLVLITCGGTYDAARGGYQENFVVKARPIS"
     gene            complement(4684..5616)
                     /locus_tag="VH419_04185"
     CDS             complement(4684..5616)
                     /locus_tag="VH419_04185"
                     /inference="COORDINATES: protein motif:HMM:NF025702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4397 domain-containing protein"
                     /protein_id="HEY7042850.1"
                     /translation="MFHRPFAFSPGFAGRALLAGAVVPLVLLLQAVPANAGTAGQHRH
                     HAAATSVYVIQGVLGTTVGVTIDGKTVSSALAEKGISGPVDLGRGEHSVQFTSDDWTV
                     SKMFQVDHSSMDVVLHWPADKTAKPVVTVYTNDLRPVPADTGRITVAHTAVVPPADVR
                     VDGKVVFANIANGEFITAEVPAGTYSVDIVPTGQHGNPVFGPVDLPVKADALTRVFAV
                     GAPKNNGMDAVVQTFPLQQKGSPRPTQVDAGAAGLVSPGMAVGLVGDQDNARGSGRWV
                     DPSLVLAGIATVLLVTGGAGFAAVRPRRRDATGP"
     gene            5824..6480
                     /locus_tag="VH419_04190"
     CDS             5824..6480
                     /locus_tag="VH419_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042851.1"
                     /translation="MAEMIRGQARPLVGRVMGAGIALVLVAALSGCHPSADGGKPNKP
                     AGHIQLSAVLDDQGRVLDVTERVTFPSDVYSFTVSIDRPDRTGTGADFDPWIERLVVE
                     ADQNQPVQRLSYIWKPTGVSLAWPARTVTLRYRMAGATYHPTPSVPGRFLAYVTGISI
                     PSTEGFSRTITIENALNLACAEPGGQLTVCGTETGGTWSVTVPAGADDTGVYAQVETQ
                     "
     gene            complement(6515..8107)
                     /locus_tag="VH419_04195"
     CDS             complement(6515..8107)
                     /locus_tag="VH419_04195"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HEY7042852.1"
                     /translation="MPFPRSTRTSDDVVAPTLADPLVRAATTRVGGPIGRHALAGRGW
                     WTVLRVTLLIATVCFGLGIVQKSPCVVTRWSEAATPKAFSHLCYSDISGLYAGRGLAE
                     GIFPYTPVDELPATAVPVTSTDRHQVSIEYPVLTGLWMGVMGEVTHLIGKDPDLSDTP
                     HSEVGNNLDVQHDSAVFWGVNAIGFFVVLLVALTLLVAAQRRRPWDAAYIAAAPVLAL
                     NAMINWDVLAIGMVAGALWGWATRRPVVAGIFIGLGAATKLYPMFLLGPLLVLCLRER
                     RMDAWTQTAAAALVAWLAIDLPVFLWSPDEFLWFWQFNAGRGPDFGSLWLVGSIYGHT
                     ASAHAINVTTWLFFGTACVAVGAMGLFAPRRPRLPQLMLLLLIAFLLVNKVYSPQYVL
                     WLLPLAVLARPRMRDLLIWQTCEILYFFAVWMHIANFFVASGDRDWIYAIAIFVRVAG
                     QLYLAAVVVRDIYSPWHDPVRTDGLSDDPLGGILDQGIDSQEYDTPESRGRDWWRPDV
                     QPSHRSQASSDEPTERDPIYAP"
     gene            complement(8276..>8788)
                     /locus_tag="VH419_04200"
     CDS             complement(8276..>8788)
                     /locus_tag="VH419_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042853.1"
                     /translation="DQRVVSSWFTGFTPQLSTAVTLTRGDGTKPLDGYLDTFFGADYP
                     TLTWTEYMKVALAGEPVKDFPDPAELHGENPTFSPPPTTYQPTTAAPSTSAPPATTSE
                     PPSTSTPPPTTPPTTSTPPPTEPTTEPTEPTEPTGPTEPTTPTTTTTTTTTPPPEGAA
                     QPNARPPDDN"
BASE COUNT         1411 a         3008 c         3041 g         1328 t
ORIGIN      
        1 cgctgccgca tcccgggccc gactccggcg ttcccggagc acgcgtttcc gatgacgccg
       61 accgaggtgc ggacccacat ccgcttcaac aggtacgagc ggaaggcctg ccgccagggc
      121 ggcagcacca ttctcgacca catgacgaca gcagacacgg cacgcgacat ggacctcatc
      181 cggcaggcgg tcggggagga ccagctcagc tactacggga tctcgtacgg cacgtacctc
      241 ggcgcgacgt acgcccgact gttcccgcac caggtccgtg cgctgatcgt ggacggcgta
      301 ctcgaccccg tcgcgtggtc caccggtcgc aacgggagtg gcacggtgat cccgttctcg
      361 acccggctgg gcagcggacg cggcgccgcc gacgcgatgc tgtcggctct cgccctctgc
      421 gaccaggtcg gtcaggagcg atgtgccttc gccggcaacg cccggcacaa gtggcgtcag
      481 ctgacgaacc gcctccggaa ggagccgctc gagttcccag acggcagcac gttggcgtac
      541 gccgatctga tcgatggcgt cctgggtggt ctgtactcca gcagcgacta ccggttcctc
      601 atgcgcgacc tccagcggct gtggacccgg acgaacccgc cgacgaaggc ggtcgtcgcc
      661 gatgacgaga tccgcgccgt gcgcgaccgt ctcgtgcagc gggaacggcg actcccgtac
      721 ggctatggac tccccttctt ccccagcttc gagggcgtcg cctgttccga cacgctgaac
      781 cccagcgatc cagccgcatg gcaacgcgcg gccgacaggt ccgaggccac gcagccatgg
      841 ttcggccgcc tgtggacgtg ggtctccgcg gcctgcccca actggccggg ctcggccgcc
      901 gacgcgttcc gcggaccgtg gcacggcacg acggccaacc ccgtcctcct ggtcggcaac
      961 gcccacgacc cggcgacgcc gttcagcgga gcagcggcgt tgcaccagct gctcgacggc
     1021 tcgcgactct tccgcctcga cggttggggg cacggtgcgc tgggcgagag ccgttgcgtc
     1081 accaaggtgt tcgacgccta cctcgtccag ggcacactgc ctgccgtcgg caccgtctgc
     1141 gaacccgacg acctgctgtt cccggtccgg cactcgacct gacgccgccg gccgctcagg
     1201 ctccatcgac gggttcgagg agcatctcca gctcgagttc gtcgcgcagc ggtatgccgt
     1261 cctggccgat ctcggcgatg gacggcttca gccggcgaga cagggcgagc gcgtcgcggt
     1321 gcagggcggc caaccgcagt ccgcgccgct ggtagaaccg cagcgcgcgc atgttgtcgt
     1381 tcgtcgtgat cagccagagc cgacggcatc cgtgcgctcg agcctcgtcg acgacggccg
     1441 agagcagcga cgagccgact ccggttccgc cgacgaacgc gtcgatcgtg acgacctcgc
     1501 aggcatcgtc ttggatgtcg aacgtcgcca gccccacccg ctcaccggct cgctcggaca
     1561 ccagcgccgg cagggccgac gcgtcgacca ggacaccgag tcgggcggcg tacaccgacc
     1621 cccacaggtc gacgagcctg gccgccaccc aggcccgctc gtcgggcgtc gaacggcgta
     1681 cggtcagggc gccggcgggc gaggccatgg cggcaccgta ccgcgaccgt cggcaccgct
     1741 tcgcgaagag ccgggtccgg tagcgttgcc cggcgtgagt ccgatcgacg tgcgcgcgat
     1801 cagcgccgcg gagcacctcg ggttcatcca ggcccagcca cgtgccagct tcctgcagac
     1861 acctgcctgg ggcggggtca agcgcgactg gaaacacgag tcgatcgggt ggtatgacgc
     1921 cggccggctg gtcggcgtcg gactggtgct gtaccggcag ctgccgcgga tccagaggtt
     1981 tctcgcgtat cttcccgaag gcccggtgat cgactgggcc agtaccgacc tcggttcttg
     2041 gctctccccg atgacggcgc atcttcgggc cgccggcgcg ttcggcatcc ggatgggccc
     2101 gccggtcgtg gtccgcagct ggaccgcaga caccgtcaag cgagccatgg cagacgacac
     2161 gcatggccgc ctcggcgacg tcccgccgga cagccgcaac gacgtgggcg gtgacgtgca
     2221 ggcgaccttg cgcaccctgg ggtggcggcc gctggtctcc acaggcggct tcaccgcggg
     2281 acaaccgcag tacgtcttcc aggtgccgct tgccgggcgt tccgaggagg acgttctgcg
     2341 cggcatgaac cagttgtggc ggcgcaacat caagaaggcg gacaaggccg gagtcgtggt
     2401 gagccaagga aaggccgacg acctcgccgc gttccacgcc ctgtacgtcg agaccgctga
     2461 acgggaccac ttcaccccgc gtccactcgg ctacttccag gacatgttcg ccgagatggg
     2521 ccaggagggc ccggacagga tccggctcta tctcgcccac cacgaggacg atctcgtcgc
     2581 gtcgaccaca atggtgcgcg tcggtgcgca cgcgtggtac tcctacggtg cttcctccac
     2641 agcgaagcgg gacgtgcgcg gctccaacgc gatccaatgg cagatgatgc gcgacgcgat
     2701 ggctgccggg gctgacgtgt acgacctgcg ggggatcacg gacacgctgg accctcacga
     2761 tcctcacgtc ggactgatcc agttcaaggt gggcaccggc ggcgatgcgg tcgagtacgt
     2821 gggcgagtgg gatctgccgc tgaaccgcct tctctacaag ggtttcgccc tctacatggg
     2881 caggcggtga cgtcccttgc cgctcacgct gtacgtcgac gggccacgat ggcggtcaca
     2941 cctgcgcgcc acagccgacg cgaaccgggg gatcgttccg gtcgcgaagg gcaacggcta
     3001 cggcttcacc atcggccggc tcgcccggcg ggccgagtgg ctgggcgtcg acatgctggc
     3061 ggtcgggacc tacgcagagg tggccgaggt ccggcagcgg ttcagcggtg acgtcctggt
     3121 gttggagcct tggcggccgt tcctcccgac cttggtgtac gacgaccgac tgatccacac
     3181 cgttggtcgc gcccaggacc tcaaggcgtt ggcggacatc ggcgacacac cgcgtgtcct
     3241 gctcgaaacg ctcacgtcga tgaaccgcca cggattccag ctcgacacgc tggccggcgc
     3301 cgccgcgact gctcgcggcg tacacatcga gggccacgct ctgcatctgc cgctcgggga
     3361 gggccacctg gacgaagtgg agcgttgcct tgcgacggcg cccgagaagc gctggtacgt
     3421 ctcccacctc actgccgccg agctgacgca actgcgcacg cgacaccccg gcgtcgagtt
     3481 ccgtccccgg atcgggacgg cgctgtggct cggggacaag caggcgctgt cgccacgtgc
     3541 gaccgttctc gatgcgcgtg acgtgcaacg cggcgagagg gcaggctacc gaggccgtcg
     3601 cttcagcaag ccgggcaccc tgctcgtcgt gtcgggggga accacgcacg gggtcgcgct
     3661 cgaggcgccg actccggcgt catcggcccg ccagcgcgcg gtggcggtgg cgcggggtgg
     3721 gctcgaggca accgggcgag cattgtcacc gttcgttgtc gacggtaagc agcgctggtt
     3781 cgtcgagccc ccacacatgc aggtcagtct ggtcttcgtc ccggccggtg cacgcgcgcc
     3841 gggggtcggc gacgagatcg acgtccaggt gcgcttcacg acgacgaggt tcgacgcgat
     3901 cgagatcagc tgatcggtcg agccttgacc acgaagttct cctggtagcc gccacgtgcc
     3961 gcgtcgtacg tcccaccaca ggtgatgagc accaggcttt cctgtccggc tgcggagaac
     4021 agcgacgacg actcggccag cgaggccttc ggcaccagtc gggcactgac cacccggtac
     4081 tcctgcgaaa gccgactggc accaagagcg agatggtcgc ccggtcgcgc ggccagcagc
     4141 tcggcgaaag ctcccagccc ctgcgtgaac gagtccacgt gcgctgcaac cacgatggat
     4201 ccgagcggat cgccgatgcg ggcactgccg ttccaccagc cggcccgccg gacatcgatg
     4261 ggcagtcgga gcgagccgtc gtcaccggtg acggcgacgt cgaccgggac cgtccgaccg
     4321 ctctgcagca cgagccgggt gggccgctgc ggatgtacct gagaaggtgc ctggcgcgcg
     4381 accctcgccg aggaggggta tgtcgggccg gcggctggcg gtgatcctgc gccggtcgac
     4441 gtggcagctc tcgacgcagg ccttggtgtt ggtgacgctg ccggaccctg gcgcgcaagc
     4501 acggtcggcg gcgagtccac cggcggtccc gcggtctggt gttgggccga cgggtccgca
     4561 gccctcgaat cgtcgaagcc ggcgagcgcc catcgagccg ccgatgcaac gagcagaccg
     4621 accgctgcga cggcgagcac cggccgcaca ccagatgggc gacggcggtg accgcccatc
     4681 agttcagggt cccgtcgcgt ctcgacgtcg aggacgcacc gctgcgaagc cggcaccgcc
     4741 ggtcacgagg aggaccgtcg cgatccccgc gagaacgagc gaagggtcga cccaacgacc
     4801 tgagcccctg gcgttgtcct ggtcacccac cagtccgacc gccatcccgg gagacacgag
     4861 tccagcagca cctgcgtcca cctgggtggg gcggggcgag cccttctgct ggagtgggaa
     4921 cgtttggaca acggcatcca tcccgttgtt cttgggagca ccgacggcga agacccgggt
     4981 caacgcgtcc gccttcaccg gaaggtccac cggtccgaag accgggttgc cgtgctggcc
     5041 ggtggggacg atgtcgaccg aatacgtgcc agccggtacc tcggcggtga tgaactcacc
     5101 gttggcgatg ttcgcgaaca ccaccttccc gtcgactctg acgtcggccg ggggcacgac
     5161 cgccgtgtgg gcgaccgtga tccggcccgt gtcggccggg accggccgca ggtcgttggt
     5221 gtagaccgtg accaccggct tcgctgtctt gtcagcaggc cagtgcagga cgacgtccat
     5281 gctcgagtgg tcgacctgga acatcttcga gaccgtccag tcgtctgacg tgaactggac
     5341 ggagtgctcg ccccgcccca ggtcgacggg tccggagatc cctttttccg caagggccga
     5401 gctcactgtc ttgccgtcga tcgtcacccc gacggtcgtt cccagaacgc cttggatgac
     5461 gtagacggat gtggccgccg cgtgatggcg gtgctgcccc gccgtccccg cattcgcagg
     5521 cactgcctgc agcagcagga cgagcgggac gactgccccc gcaagcagag cgcggccggc
     5581 gaagccaggg ctgaacgcga atgggcgatg gaacatgagc tacccccctg ctacaagtcg
     5641 acacggaccc cccgcccgcg accagaaccg tctgcacaag cgtgcttact gtcagtggcg
     5701 cgcaccggtc tcttcgcaca ccatgtgcgt gagttctggc caattggtta tatcccctgc
     5761 attctgtaaa cgtccagagc ggaatgtgta gcggcgcccg acggccgggt cgccgtggag
     5821 ggcgtggccg agatgatccg agggcaggcc cgcccgctgg tcgggcgggt gatgggggcg
     5881 gggatcgcac tcgttctcgt cgcagcactc agcggttgcc acccttcagc ggacggcggt
     5941 aagccgaaca agcccgccgg gcacatccag ttgtcggcag tgctcgatga ccagggccgc
     6001 gtcctcgacg tcaccgagcg cgtcacgttt ccctcggacg tctacagctt cacggtgtcg
     6061 atcgaccggc cggaccgaac ggggaccggc gccgacttcg acccgtggat cgaacgcctc
     6121 gtggtggagg cagaccagaa ccagccggtc cagagactga gctacatctg gaagccgacc
     6181 ggtgtctccc tcgcttggcc ggcccgcacc gtgacgctgc gctatcggat ggccggggcg
     6241 acgtaccatc cgacaccctc ggttccaggg cgcttcctcg cctacgtcac cgggatcagc
     6301 atcccgagca ccgaggggtt ctctcgcacg atcaccatcg agaacgcact caacctcgcc
     6361 tgcgccgagc cgggcggcca gctgacggtg tgcgggaccg aaaccggtgg cacctggtcg
     6421 gtgaccgtgc ccgccggcgc cgatgacact ggggtgtacg cacaggtgga gacccaatga
     6481 cttcaccgac gacgctgcca tcgacggcga gcgatcaggg cgcgtagatc gggtcccgct
     6541 cggtcggctc gtccgagctc gcctgcgacc gatgagacgg ctgcacgtcg ggccgccacc
     6601 agtcgcggcc tcggctttct ggggtgtcgt actcctggct gtcgatgccc tggtcgagaa
     6661 tcccgccgag cgggtcgtcg gagagcccgt ccgtccgtac gggatcgtgc cagggcgagt
     6721 agatgtcacg aacgactacg gcagccaggt agagctgacc cgccacccgc acgaagatgg
     6781 cgatcgcgta gatccagtcg cggtccccgg acgccacgaa gaagttcgcg atgtgcatcc
     6841 agaccgcgaa gaagtacaag atctcgcagg tctgccagat gagcaggtcg cgcatgcgag
     6901 gtcgtgcgag cacggcgagc ggcagcagcc acagcacgta ctgaggcgag tagaccttgt
     6961 tgacgagcag gaacgcgatg aggagcagca gcatgagctg cggtagccgg ggccgtcgag
     7021 gggcgaacag gcccatggcg ccgaccgcga cgcaggccgt gccgaagaag agccaggtcg
     7081 tcacgttgat ggcatgagcg ctggcggtgt gaccgtagat cgacccgacg agccacagcg
     7141 acccgaagtc gggaccgcga ccggcgttga attgccagaa ccacaggaac tcgtccggtg
     7201 accataagaa gaccggcagg tcgatcgcca gccaagcaac cagggctgcg gcagcggtct
     7261 gcgtccacgc gtccatccgg cgctcgcgaa ggcagagcac gagcagcggt cccagcagga
     7321 acatcgggta cagcttcgtg gcggctccga gtccgatgaa gatacccgcc acgacagggc
     7381 gtctcgtcgc ccagccccac agtgcgccgg cgaccatccc gatcgcgagc acgtcccagt
     7441 tgatcatcgc gttcagggcc agcaccggtg cggcggcgat gtacgccgcg tcccatgggc
     7501 gtcgtcgttg tgctgcgacg agcagcgtca gcgccacgag caggaccacg aagaacccga
     7561 tggcgttgac cccccagaag acggcactgt cgtgctggac gtccaggttg ttccccacct
     7621 cggagtgcgg cgtgtcggag aggtccgggt ccttgccgat gaggtgggtg acctcgccca
     7681 tgacgcccat ccagagcccg gtgagcaccg ggtactcgat ggacacctga tgccggtcgg
     7741 tgctggtgac cggcacggcg gtcgctggga gctcgtcgac cggcgtgtac gggaagatcc
     7801 cctccgccag gcctcggccg gcgtacaggc cgctgatgtc ggagtagcac aggtgcgaga
     7861 acgccttcgg ggtcgccgcc tccgaccacc tcgtgacgac acacggcgac ttctgcacga
     7921 tgccgagtcc gaagcagacg gtggcgatca acaacgtgac ccgaagcact gtccaccagc
     7981 cacgtccggc gagcgcgtgc cggccgatcg gtccaccgac ccgagtcgtc gccgcccgaa
     8041 ccaacggatc ggcgagcgtc ggcgcgacca cgtcgtcact ggtacgcgtg ctccggggga
     8101 acggcacgca cacatggtgc ctcatgcctg acggccagca tcgcctgccc gtggtgcggt
     8161 caccccgcgc cgctctccgg gggaggtgct cgttcagcga ggtaccgagg tggcaatcgc
     8221 ggtgcgacac gccgccgaaa tccaacgaaa cctgccacct cggcggtggt gggactcagt
     8281 tgtcgtcagg tgggcgtgcg ttgggctggg cggcgccttc gggaggcggc gtggttgtcg
     8341 ttgtggtcgt ggtcgtcggc gtcgtcggct ccgtcggccc agtcggctct gtcggctctg
     8401 tcggctctgt cgtcggctct gtcggcggcg gcgtcgaggt ggtcggcggt gtcgtcggcg
     8461 gcggtgtcga ggtcgacggc ggctccgacg tggtcgccgg aggagcggac gtcgacggcg
     8521 cggccgtggt cggctggtag gtcgtgggag gcggtgagaa cgtcgggttc tcgccgtgca
     8581 gctcggccgg gtctgggaag tccttcaccg gctcacccgc cagggcgacc ttcatgtact
     8641 ccgtccacgt cagcgtcgga tagtccgctc cgaagaacgt gtccaggtag ccgtcgagcg
     8701 gcttggtgcc gtcaccgcgc gtcagcgtga ccgcggtgga cagctgcggc gtgaagccgg
     8761 tgaaccagga cgagacgacc cgctggtc
//
LOCUS       DATBFZ010000140        10917 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_2716283, whole genome shotgun sequence.
ACCESSION   DATBFZ010000140 DATBFZ010000000
VERSION     DATBFZ010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 10917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10917
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2716283"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1452)
                     /locus_tag="VH419_04205"
     CDS             complement(<1..1452)
                     /locus_tag="VH419_04205"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEY7042854.1"
                     /translation="MHEPTHRRLRRLGAVAASGLLVAAVVSAIDDAQAQPATSAGAAT
                     SATHSDKTSYARGNYVVVMSGQPAAVYHGGLAGYRATAATKTAKFNSASKAAHAYRAY
                     LTERQNTAARAVGATPYYHYSASLNGFAAKLSASQARQLSHRAGVLAVVPDTIRHADT
                     TKTPGFLGLTGPGGVWRRLGGTDGVNGAGKGVIIGIIDSGINDDNPVFHSVGDGVPAD
                     WHGVCDTGADDTFACNDKLIGGRYFDAGADVIPEEFESPDDFHGHGTHVAATAGGNYG
                     VHSVINGIDFGATSGMAPAAKLAAYKVCWELADHTNCNASTSDSVAAIDAAVSDGVDV
                     LNYSISGTQDNPIDPVELAYMYAADAGVFVAASAGNSGPGASTVAHPSPWLTTVAAAT
                     HFNYESTVLLGDGTRMVGASISPTGVDESPLVYSGDIPAAGQDPADAALCMPDSIDPA
                     QATGKIVICDRGVNARVEKSSVVQAAGGVGMVLV"
     gene            complement(1626..2339)
                     /locus_tag="VH419_04210"
     CDS             complement(1626..2339)
                     /locus_tag="VH419_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEY7042855.1"
                     /translation="MPGRHHGRHVAPRHRAPRSVKQTLGRFVGDLAGTLPASGVTRTV
                     AFVGAAGVAFGLGVFTVDAEANPDDTTAAVADSFDATTSLSDDAIKARHRHSADEVSR
                     SADRSTSALVQRETKARHLAAAKQDMAGSISKTVAATDPRDIAKSMLDDHGWSSDQFS
                     CLDSLWVSESNWNVYATNSYSGAYGIPQALPAEKMASAGPNWRTDPATQIEWGLDYIR
                     SSYGSPCSAWYFKESNNWY"
     gene            complement(2549..3874)
                     /locus_tag="VH419_04215"
     CDS             complement(2549..3874)
                     /locus_tag="VH419_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012923162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEY7042856.1"
                     /translation="MSSTGPAKSPPAGVRTFVIDTSVLLADPAALKRFHEHHVVLPVV
                     VITELEAKRAHPELGYFARSALRLLDELRLLHGQLDCPVPMGDAGGTLRVELNHSDPT
                     VLPAGFRLGDNDTRILSVALNLRAEGADVTLVSKDLPMRVKASSVGLPAEEYRAELAV
                     ETGYTGMSEIDVAAADLDALYDEGRVDVEAARSIPCHSGLVLISERGSALGRVTADKQ
                     VRLVRGDREAFGLRGRSAEQRVALDLLLDPDIGIVSLGGRAGTGKSALALCAGLEAVL
                     ERRQHKKVVVFRPLFAVGGQELGYLPGSESEKMQPWAQAVFDTLGAVTSSDVIEEVLA
                     RGMLEVLPLTHIRGRSLHDAFVVVDEAQSLERNVLLTVLSRVGANSRVILTHDVAQRD
                     NLRVGRYDGVVAVVEKLKGHPLFAHITLTRSERSPIAALVTEMLEDVSI"
     gene            3813..4106
                     /locus_tag="VH419_04220"
     CDS             3813..4106
                     /locus_tag="VH419_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042857.1"
                     /translation="MSITNVRTPAGGLFAGPVELTVDRVADRVDVDAALRWFADRAGA
                     DVRPLEPVARATLIPSQTAREHRLARPSVSRRTGTNLPQGTMPALIDGALGLP"
     gene            complement(4311..5075)
                     /locus_tag="VH419_04225"
     CDS             complement(4311..5075)
                     /locus_tag="VH419_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012923164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEY7042858.1"
                     /translation="MASARSLVYDLYERRLMRQIDPEQIPGHIGVMCDGNRRWARFSG
                     EEVEGGYIAGADKIEEFLGWCDDLHVELVTLWLLSTDNLARPADEIEPLLRVIEDLVD
                     ELADAGRWRVQSVGALDLLPAATAQRLKDAADRSAHVAGMTVNVAVAYGGRRELADAV
                     RSLLHEHAEQGTTIEELAEFIDVEHIAEHLYTRGQPDPDLVIRTSGEQRMSGFLLWQS
                     AHSEFYFCEAMWPDFRRVDFLRAVRAYGARERRYGT"
     gene            5192..5869
                     /locus_tag="VH419_04230"
     CDS             5192..5869
                     /locus_tag="VH419_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020578408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEY7042859.1"
                     /translation="MIVQATERLREAAREIKPHLRGWLHLGTFPLSLAAGIVLVSLAP
                     DTRTRTACAIFGLSASLLFGCSALFHRGHWGPRLHGVLRRLDHSNIFLLIAGTYTPFA
                     LMLLDGTSARILLSLVWGGALVGIGFRIFWIGAPRWLYLPVYIALGWAAVFWLGEFAS
                     AAGPAVLTLIIVGGGLYSVGGLIYGIKRPNPIPRWFGFHEVFHTFTIAAFVVHYVGVS
                     LVVYGQR"
     gene            complement(5895..8189)
                     /locus_tag="VH419_04235"
     CDS             complement(5895..8189)
                     /locus_tag="VH419_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020387576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEY7042860.1"
                     /translation="MTVEDAERQVLRTEDQHLITGRTRWTANVRLPGMLHLTFVRSPM
                     AHARVTIDVDAARRHPGVVGVWTGEDVARWCPQLPGFEDEPSMPLIAVDTVRYVGEAV
                     AVVVARSATSAVDAAELVAVDYDELPVVTDVESAVVDGAPQLHPDVVRNVASDRHRDH
                     GDVEAAMEAADVVVRRRFEQPRVFTAAMEPRAVTVMPDGNGFTVWVSTQTPHIVKHFL
                     AEATGIEPDRLRVVAPDVGGGFGGKFFYAEEVVALLAAREHNRSVAWFASRSADLQTT
                     FHGRALVQELTIAATSDGEITGLDVSLLEDAGAYLSPIGPSAMGGARMYPGIYRVANY
                     RFRCQTVLTNKTPVGAYRGAGRPEATYAIERMVDELAATLAVDPVELRRKNWIPADAF
                     PYKTSGGSTYDVGDYASTTDAMLELGDYAGWRKRQVEQNVDGAGVRLGIGVSTYVEAC
                     GGGIRYDDGAVETASVRLTPEGAEAVVGTSAFGTGHLTSWAQVVARELGLDVAAIKVV
                     QGDTDRAPHGFDSYGSRSLSVVGSALHGAAVEVRRRAMDVAARVLECDPGDLEFEAGT
                     FTVRGTSASTTLRDVALASYNDRTLAMDGFEPGLGCTRTSDLKIATYPYGAHLAVVEV
                     DVDTGAVRLVDYVGVDDVGNVVNAMIVEGQVHGGVVQGIAQAMFEEVSYDAQANLLTP
                     SFTEYAMPSAADLVTMRTDRRVTPATTNPLGTKGVGEAGAIAAPPAVMNAVLDALRPL
                     GVVDVPMPCTPYRVWLSCQNAMGL"
     gene            complement(8219..10201)
                     /locus_tag="VH419_04240"
     CDS             complement(8219..10201)
                     /locus_tag="VH419_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020577223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEY7042861.1"
                     /translation="MANRLATATSPYLLQHADNPVDWREWSAEAFTEASERDVPVLLS
                     VGYAACHWCHVMAHESFEDEATAAYMNEHFVNVKVDREERPDIDAIYMEATQSMTGQG
                     GWPMTCILTPDGAPFFAGTYFPRVPQPGMPAFRQVLAAVSQAWRDRREEINRIGSDVT
                     AYLRQLPQPESAASEDVTAQAVDELTRSFDDVHGGFGRAPKFPPSMICEFLLRHAARR
                     DDAHALDMVERTLEAMARGGIYDQLGGGFARYSVDDRWDVPHFEKMLYDNAQLLRLYA
                     HWHRQTGNPLAARVARETAGFMIRELGTAGGAFASALDADSDGAEGVFYVWTPEQLDD
                     VLGTDDGAFARRVFGLREGGNFEHGTSTLRLDQDPEPGETHRLADVRRRLLDARDQRT
                     RPARDDKVVAAWNGLAIAALTEAGMILDEPDFVVAATAAAAMLVEVHFEDGRLRRVSR
                     DGRPGRPAGVLEDYACLADGMLTLFGATGEARWYAVARDLVDAIEAHFADGDGGFFDT
                     ADDAETLLKRPQDSTDTASPSGQSMSLTVLTTMHGLTGADRFSAAAERLANRLSGLAQ
                     RAPRFAGQTLCALEAMLDGPRQVAVVGEDDDGRADLLRAAHRLVHPGLVVAVGDGNEA
                     TVPLLESRPLLAGRATAYSCQHFVCDLPVTDPAALR"
     gene            10515..10736
                     /locus_tag="VH419_04245"
     CDS             10515..10736
                     /locus_tag="VH419_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042862.1"
                     /translation="MAALDTEKETPVGADLLAARTILMLGEAYIQDGIERGKQLRTME
                     QGVSRLNAHIQPTIGGVRSRSGGSSTAAR"
     gene            10736..>10917
                     /locus_tag="VH419_04250"
     CDS             10736..>10917
                     /locus_tag="VH419_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042863.1"
                     /translation="MDKGSKTLFSTRGREDLRGQLAAMRKLAWRLGWLDRSIDPLDGL
                     EIGRASQRVARCNSAIR"
BASE COUNT         1658 a         3795 c         3738 g         1726 t
ORIGIN      
        1 gacgagcacc atgccgacac caccggcggc ctggaccacg gagctcttct cgacccgggc
       61 gttgacaccg cggtcgcaga tcacgatctt gcctgtggcc tgcgcggggt cgatcgagtc
      121 cggcatgcag agcgcggcgt ccgcggggtc ctgaccggca gcggggatgt caccggagta
      181 caccagcggc gactcgtcga caccggtcgg gctgatcgag gcaccgacca tcctggttcc
      241 gtcgccgagc agcacggtcg actcgtagtt gaagtgcgtc gcggccgcga ccgtggtcag
      301 ccacgggctc gggtgcgcca cggtcgacgc gccaggaccg ctgttgccgg ccgaggcggc
      361 gacgaacaca cccgcgtcgg cggcatacat gtaggccagc tcgaccgggt cgatcgggtt
      421 gtcctgcgtc ccgctgatcg agtagttgag gacgtcgacg ccatccgaca cggccgcgtc
      481 gatcgcggcg accgagtccg acgtcgacgc gttgcagttg gtgtggtcgg ccagctccca
      541 gcagaccttg tacgccgcga gcttggcggc cggcgccata cccgacgtcg caccgaagtc
      601 gatgccgttg atcaccgagt gcacgccgta gttgccgccg gcagttgcgg cgacgtgggt
      661 gccgtgaccg tggaagtcgt cgggtgactc gaactcttcg gggatgacgt ctgccccggc
      721 gtcgaagtag cgcccaccga tcagcttgtc gttgcatgcg aacgtgtcgt ccgcgccggt
      781 gtcgcagacg ccgtgccagt cagccggtac gccgtcgccc acggagtgga agaccgggtt
      841 gtcgtcgttg atgccgctgt cgatgatgcc gatgatgacg cccttgcccg caccgttgac
      901 accgtccgtg ccgcccagcc gccgccagac cccaccgggg ccggtgaggc cgaggaaccc
      961 aggcgtcttc gtcgtgtcgg catgccggat cgtgtccggg acgacggcca gcacgccggc
     1021 gcgatgcgac agctgccgcg cctggctcgc ggacagcttc gccgcgaaac cgttcaggga
     1081 cgccgagtag tggtagtacg gcgtcgcgcc gacggccctc gcggcggtgt tctgacgttc
     1141 ggtcaggtac gcgcggtagg cgtgggccgc cttgctggcc gagttgaact tcgcggtctt
     1201 ggtggcggcg gtggcccggt agcccgccag cccgccgtgg tagacggcgg ccggctggcc
     1261 ggacatcacg accacgtagt tgcccctggc gtacgacgtc ttgtcggaat gggtggccga
     1321 cgtggcagcg ccggcactgg tggccggttg cgcctgcgcg tcgtcgatgg cgctgacgac
     1381 ggccgcaacg agcaggcccg aagccgcgac agccccgagc cgccgcagtc ttcgatgggt
     1441 gggttcgtgc acgaaagtcc cctgtcagtg gatctccggg actggacggt tgtccagccg
     1501 cgaaaaatca acctgctggc aggagaaaag ccgagagtac agcgctaaga gttatgaatc
     1561 tgtgatcttt caacagatac cagcatcgga gaggtatgta ccgtttgcgc cgcgaggccg
     1621 agcggttagt accagttgtt cgattccttg aagtaccagg cgctgcaggg tgagccgtag
     1681 gacgaccgga tgtagtcgag gccccactcg atctgggtgg cggggtcggt ccgccagttc
     1741 gggccggccg aggccatctt ctccgcgggc agcgcctgcg ggatcccgta cgccccggag
     1801 taggagttcg ttgcgtagac gttccagttg ctctcgctca cccacaacga gtcgaggcag
     1861 gagaactggt cgctgctcca gccgtggtca tcgagcatgg acttcgcgat gtcgcgcgga
     1921 tcggtggccg cgacggtctt ggagatgctg cccgccatgt cctgcttggc ggcagccagg
     1981 tgtctggcct tcgtctcccg ctgcacgagc gccgaggtcg accggtcggc ggatcgactg
     2041 acctcgtcag cgctgtgccg gtgccgagcc ttgatcgcgt cgtcggacag gctcgtggtg
     2101 gcgtcgaacg agtcggcgac cgcggcggtc gtgtcgtccg ggttggcctc ggcatcgacg
     2161 gtgaacacgc cgagaccgaa cgcgacgccg gccgccccga cgaaggcaac cgtccgggtg
     2221 acgcccgacg cggggagcgt gccggcgagg tctccgacaa agcgccccag tgtctgtttg
     2281 acggaccggg gcgctcgatg tcgtggggcg acatgcctgc cgtggtgcct tccgggcacg
     2341 cagcatcact cctcgagggg tggaagaccg ggaccagcct gagggcgtcc tcgacgagag
     2401 tcaaaccaat cactcgatcc ctgtggattg tgtgacggac tcgacactca ggccggtggc
     2461 ctcgtcggac gaaggcggcg taccccgcga cccgacgccg aaacgcacgc gacagatcag
     2521 acgagggtgc gaagtcaggt acgccgcatc agatcgacac gtcctccaac atctccgtca
     2581 ccagcgccgc aatcggtgat cgctcggacc tggtgagcgt gatgtgtgcg aacagcggat
     2641 ggcccttgag cttctcgacc acggccacga caccgtcgta ccgaccgacg cggaggttgt
     2701 cacgttgcgc cacgtcgtgg gtcaagatca cccgcgagtt cgcaccgaca cgcgacagca
     2761 cggtgagcag cacgttgcgc tcgagcgact gtgcctcgtc gacgaccacg aacgcgtcgt
     2821 gcagggatcg gccccggatg tgcgtgaggg ggagaacctc gagcatgccg cgggcgagaa
     2881 cctcttcgat cacgtcactg ctggtcaccg caccgagcgt gtcgaagacc gcctgcgccc
     2941 agggctgcat cttctccgac tcgctgcccg gcaggtagcc gagttcctgc ccgcccaccg
     3001 cgaacagcgg ccggaagacg acgaccttct tgtgctggcg gcgctcgagg acggcctcga
     3061 gcccggcgca gagcgcgagc gccgacttgc cggtaccggc acggccgccg agcgacacga
     3121 tgccgatgtc ggggtcgagc agcaggtcga gggcgacgcg ctgttcggcg gaccgcccgc
     3181 gcaggccgaa ggcctcgcgg tcgccacgca ccagacggac ctgcttgtcc gccgtgaccc
     3241 tgccgagcgc gctaccgcgc tcggagatga gcacgagacc cgagtggcac gggatcgacc
     3301 gggccgcctc gacatccacc ctgccctcgt cgtacagcgc gtccaggtcg gcagcggcga
     3361 cgtcgatctc gctcatgccc gtgtagccgg tctcgacggc gagctcggcg cggtactcct
     3421 ccgcgggcag ccccaccgac gaggccttga cccgcatcgg cagatccttg gagacgaggg
     3481 tgacgtccgc gccctcggcg cgaaggttga gggccacgga caggatgcgc gtgtcgttgt
     3541 cgccgagccg gaagccggcg ggaagcacgg ttgggtcgct gtggttgagc tcgacccgca
     3601 acgtgccacc ggcgtccccc atcggcacgg ggcagtcgag ctggccgtgc agcagccgca
     3661 gctcgtccag cagccgcaac gcggagcggg cgaagtatcc gagctcggga tgtgcgcgtt
     3721 tcgcctcgag ctcggtgatc acgacgacag gcagcacgac gtggtgctca tggaatcgct
     3781 tgagggctgc gggatcggcc aacaagacgc tggtgtcgat cacgaacgtg cgtacgccgg
     3841 caggtggact cttcgccggc ccggtcgagc tcaccgtgga ccgtgtggcc gatcgtgtcg
     3901 acgtcgacgc cgccttgcgc tggttcgcgg atcgggccgg agcggacgta cgtccgttgg
     3961 aaccggtggc tcgggccacg ttgatcccct cgcagaccgc gcgggagcat cgcctcgctc
     4021 ggccctcggt gtctcgtcgg accgggacga acctgcccca gggcacgatg cctgccctga
     4081 tcgatggtgc actgggcctc ccgtgacgtc ggacttccct ccggcgtcag ttcagacggt
     4141 acgacgctgg aggttgtcgg gcagcgccga cacgcggacc cctcggtgaa cgtccggcct
     4201 gtcgtttcac acgcacgcac ggcgttggtt cccggcgagg tggcaggttt ggttgcagac
     4261 acgccggttc gcgagacgcg aatgccacct cggcggggtc tggccggggg ctaggtgccg
     4321 tagcgacgct cgcgggcgcc gtacgcacgg accgcgcgaa ggaagtcgac ccggcggaaa
     4381 tcgggccaca tcgcctcgca gaagtagaac tcgctgtgcg cgctctgcca gaggaggaag
     4441 ccggacatgc gttgctcgcc ggaggttcgg atcaccaggt cggggtcggg ctgaccgcgt
     4501 gtgtacaggt gctcggcgat gtgctcgacg tcgatgaact cggcgagctc ctcgatcgtc
     4561 gtcccctgct cggcgtgctc gtggagcagc gaccgaaccg cgtcggcgag ctcgcgccgg
     4621 ccgccgtagg cgacggccac gttgacggtc atccccgcga cgtgagcgct gcggtcggcc
     4681 gcgtccttga ggcgctgcgc ggtcgcggcg gggagcaggt cgagggcacc cacggactgg
     4741 acccgccacc gacccgcgtc cgccagctcg tcgaccaggt cttcgatgac gcgaagcaag
     4801 ggctcgatct cgtcggcggg gcgggcgagg ttgtcggtgg acagcagcca caacgtgacg
     4861 agctcgacgt gcaggtcgtc gcaccaaccg aggaactcct cgatcttgtc ggctccggcg
     4921 atgtacccgc cttcgacctc ctcaccggag aaccgcgccc accgccggtt gccgtcgcac
     4981 atgacgccga tgtgaccagg gatctgctcc gggtcgatct gccgcatcag ccgacgctcg
     5041 tacaggtcgt acaccagcga tcgtgccgat gccatcgcgg acctcctggc ggacagtgat
     5101 cggggcgcgc gtgcggacgt tggaaatgtc acgtccaccg gtcgaacgtt acccctgcga
     5161 aggtggtcgt ttcccgtcta ccgtggagag catgatcgtg caggcgaccg agcggcttcg
     5221 tgaggctgcc cgcgagatca agccccacct gcgcggttgg ctgcatctcg gcacgttccc
     5281 gttgagcctg gcggccggca tcgtgctcgt cagtctcgcg ccggacacga ggacgcggac
     5341 cgcgtgtgcg atcttcggtc tgtccgcgtc gctgctgttc ggatgctccg cgctgttcca
     5401 ccggggacac tggggcccgc gcctgcacgg cgtactccgg cgcctcgacc actcgaacat
     5461 cttcctgctc atcgccggca cctacacgcc gttcgccctg atgctgctcg acggcacgag
     5521 tgcccggatc ctgctcagcc tggtgtgggg cggtgcgctc gtcggcatcg gcttccggat
     5581 cttctggatc ggtgcgccgc gctggctcta cctcccggtg tacatcgcgc tgggatgggc
     5641 ggcggtcttc tggctcggcg agttcgcctc ggcggctggg cctgccgtgc tgaccttgat
     5701 catcgtcggc ggcggcctgt actccgtcgg cggtctcatc tacgggatca agcggccgaa
     5761 cccgattccc cgctggttcg ggttccacga ggtcttccac acgttcacga tcgccgcctt
     5821 cgtcgtccac tacgtcggcg tctccctggt cgtctacggc caacgctgaa ccgacttgtc
     5881 agaagctgat ggggctatag gcccatggcg ttctgacaac tcagccagac gcggtagggc
     5941 gtgcacggca tcggtacgtc gaccacgccc agcggccgca gcgcgtcgag aacggcgttc
     6001 atcaccgcag gtggcgccgc gatcgcgccc gcctccccca cgcccttcgt cccgagcggg
     6061 ttcgtcgtcg ccggcgtgac ccgccggtcg gttcgcatcg tgacgaggtc ggccgcggag
     6121 ggcatggcgt actccgtgaa gctcggcgtc agcagattgg cctgggcgtc gtaggacacc
     6181 tcttcgaaca tcgcctgcgc gatcccctgc accaccccac cgtggacctg accctcgacg
     6241 atcatcgcgt tcacgacgtt ccccacgtcg tccacgccca cgtagtcgac cagccgcacc
     6301 gccccggtat cgacgtcgac ctccaccacg gcgaggtgcg cgccgtacgg gtaggtcgcg
     6361 atcttcaggt cggacgtgcg ggtgcagccc aggccgggtt cgaagccgtc cattgccagc
     6421 gtccggtcgt tgtacgacgc cagcgccaca tcgcgcagcg tggtcgaggc cgaggtgccg
     6481 cgcacggtga acgtccccgc ttcgaactcc aggtcaccag ggtcgcactc gagcacccgc
     6541 gcggcgacgt ccatcgcccg ccgacgcacc tccacagctg cgccgtgcaa cgccgacccg
     6601 acgacgctca aggaccgtga cccgtacgag tcgaacccat gcggagcacg atcggtgtct
     6661 ccttggacga ccttgatcgc ggccacgtcg agcccgagct cgcgggcgac gacctgcgcc
     6721 cagctcgtca ggtggccggt gccgaacgcc gaggtcccga ccaccgcctc cgccccttcc
     6781 ggggtcagcc gcacactcgc ggtctcgacg gcaccgtcgt cgtaccggat cccgccccca
     6841 cacgcctcga cgtacgtcga cacaccgatc ccgaggcgca cacccgcgcc gtcgacgttc
     6901 tgctccacct gccgcttccg ccaaccggcg tagtcgccga gctcgagcat ggcgtcggtc
     6961 gtgctcgcgt agtcgccgac gtcgtacgtc gatccgccgc tcgtcttgta ggggaacgcg
     7021 tccgcgggga tccagttctt ccggcgcagc tcgaccggat cgaccgccag cgtcgcagcg
     7081 agctcgtcga ccatccgctc gatcgcgtac gtcgcttccg gcctgccggc gccccggtag
     7141 gcaccgaccg gcgtcttgtt ggtcagcacg gtctggcaac ggaagcgata gttcgcaacc
     7201 cggtagatgc ctggatacat gcgcgccccg cccatggccg acgggccgat cggggacagg
     7261 tacgcgccgg cgtcctccaa caggctgacg tcgaggccgg tgatctcgcc atccgaggtg
     7321 gccgcgatgg tcagctcctg cacgagcgcc cgtccgtgga aggtcgtctg cagatccgcg
     7381 ctgcgggagg cgaaccaggc cacggagcgg ttgtgctcgc gcgccgcgag cagcgccacc
     7441 acctcctcgg cgtagaagaa cttgccgccg aaaccgccgc cgacgtcagg cgcgaccacc
     7501 cgcaggcgat ccggctcgat gccggtcgcc tcggcgagga agtgcttgac gatgtgcgga
     7561 gtctgcgtgg acacccacac cgtgaacccg ttcccgtcgg gcatgaccgt gaccgcacga
     7621 ggctccatcg ccgcggtgaa cacgcgcggc tgctcgaacc ggcgccggac caccacatcg
     7681 gcggcctcca tcgccgcttc gacgtcgccg tggtcccggt gccggtcgga ggcgacgtta
     7741 cggacaacgt cgggatgcag ctgcggagcc ccgtcgacga ccgctgactc gacgtcggtg
     7801 acgactggga gctcgtcgta gtcgactgcg accagctcgg ccgcgtccac ggctgaggtg
     7861 gcggaccgtg ccaccaccac tgcgacggcc tccccgacgt accgaaccgt gtcgacggcg
     7921 atcaacggca tgctcggctc gtcctcgaag cctggtagct gcggacacca cctggccacg
     7981 tcctcgcctg tccagacccc gaccacgccc gggtggcgtc gggctgcgtc cacgtcgatc
     8041 gtgacccggg catgcgccat cggactgcgt acgaacgtca gatgcagcat tccgggcagc
     8101 ctgacgttcg ccgtccagcg agtgcgaccg gtgatcaggt gctggtcctc ggtgcgcaac
     8161 acctgacgct cggcgtcctc gacggtcatg ggcaaagcgt aggggtgaag ccgcagcact
     8221 agcgcagcgc ggctgggtcg gtcaccggca ggtcgcagac gaagtgctgg caggagtacg
     8281 ccgtggcgcg tccggccagg agcggacgcg actccaggag cggcacggtc gcctcgttcc
     8341 cgtcgccgac ggcgaccacc agccctgggt ggacgaggcg gtgcgccgcc cggagcaggt
     8401 cggcgcgtcc gtcgtcgtct tcacctacca ccgccacctg gcgcggcccg tccagcatcg
     8461 cctccagcgc gcacagcgtc tgtccggcaa accggggtgc gcgctgggcg agtccgctca
     8521 gccggttcgc cagccgctca gccgccgctg agaatcggtc cgcaccggtc agtccgtgca
     8581 tggtcgtgag gacggtcaga ctcatcgact gacccgacgg cgaggccgtg tccgtcgagt
     8641 cctgtggccg cttcagcagc gtctcggcgt cgtcggcggt gtcgaagaag ccgccgtccc
     8701 cgtccgcgaa gtgcgcctcg atcgcgtcga cgagatcacg cgcgaccgcg taccaccgag
     8761 cctcaccggt cgcaccgaac agcgtgagca tgccgtccgc gaggcaggcg tagtcttcga
     8821 ggacaccggc gggacggccc gggcgaccgt ctcgcgagac gcggcgcagc cgaccgtcct
     8881 cgaagtgcac ctcgacgagc atcgctgccg ccgccgtcgc tgccaccacg aagtccggct
     8941 cgtcgaggat catcccggcc tcggtcaacg cggcgatcgc caacccgttc caggcggcga
     9001 cgaccttgtc gtcgcgggcg ggccgggtgc gctggtctcg cgcgtcgagg agccgacggc
     9061 gtacgtctgc cagccggtgg gtctctcccg gttcgggatc ctggtccaga cgcaacgtcg
     9121 acgtaccgtg ctcgaagttc ccgccctcgc gcagcccgaa gacccgacgg gcgaacgccc
     9181 cgtcgtccgt gccgaggaca tcgtcgagct gctcgggcgt ccacacgtag aacacgcctt
     9241 ccgccccgtc ggagtcggcg tccagggcgg aggcgaacgc gccaccggcc gtgccgagct
     9301 cacggatcat gaagcccgcc gtctcgcggg ccacccgtgc cgcgagcggg ttgccggtct
     9361 ggcggtgcca gtgggcgtag aggcgcagca gctgggcgtt gtcgtacaac atcttctcga
     9421 agtgcggcac gtcccagcgg tcgtcgacgc tgtagcgagc gaacccgccg ccgagctggt
     9481 cgtagatgcc gcctcgtgcc atcgcctcga gcgtgcgctc caccatgtcc agcgcgtgcg
     9541 cgtcgtccct acgagccgcg tgacggagca ggaactcgca gatcatcgag ggtgggaact
     9601 tcggcgctcg cccgaaccca ccgtgcacgt cgtcgaagct gcgggtcagc tcgtcgaccg
     9661 cctgagcggt cacgtcctcg ctcgccgccg actctggctg aggcagctgg cgcaggtagg
     9721 ccgtgacgtc ggagccgatc ctgttgatct cctcacgacg gtcacgccat gcctgggaca
     9781 cggccgccag gacctggcgg aacgccggca tgccgggctg cggcacgcgc gggaagtacg
     9841 tccctgcgaa gaacggcgca ccgtccggtg tgaggatgca ggtcatcggc cagccaccct
     9901 gccctgtcat cgactgggtg gcctccatgt agatcgcgtc gatgtcgggt cgttcctcgc
     9961 ggtcgacctt gacgttgacg aagtgctcgt tcatgtatgc cgcggtcgcc tcgtcctcga
    10021 acgactcgtg cgccatcacg tggcaccagt ggcaggcggc atacccgacg gacagcagca
    10081 cgggcacatc gcgctcgctc gcctccgtga acgcctccgc cgaccactcc cgccagtcga
    10141 ccgggttgtc ggcgtgctgc agcaggtagg gagacgtggc ggttgccaac cggttggcca
    10201 cggtgctcct ctcatggagg tggggtggtg ggccgagcct gcccgggcgt cgaccgcagc
    10261 gttccctctt gccatcgtgc caaaccgccg gaatgccgct gatcgacagg cccgctgatc
    10321 gagcgtgtcg agttgcccgc tgatcgagcc tgtcgagatc gagggccggg gtctcgacaa
    10381 gctcgaccgc cggacacccg aacgcccgct gatcatccgg tctccacgag ctcgacgccg
    10441 gaggcgagcc gcggaagcgg gtactgcggc gcgcgtcatc ggagaccgag gcctgcaaga
    10501 tcttcgatcg ggccgtggct gccctcgaca cggagaagga gacgcccgtc ggcgcagacc
    10561 tccttgccgc ccgcacaatc ttgatgctcg gcgaggcgta catccaggac ggcatcgagc
    10621 gcggcaaaca actgcggacc atggagcagg gagtgtcgcg cctcaacgcc cacatccagc
    10681 cgacgatcgg cggcgtgcgg tcgcgaagtg gcgggtcgag cacagccgca aggtgatgga
    10741 taagggctcg aagaccctct tctccacccg cggccgcgag gacctgcgtg gccagctggc
    10801 cgcaatgcgc aagctcgcct ggcggctggg ctggctggac cgcagcatcg acccgcttga
    10861 cggcctcgaa atcggtcggg cgagccaacg tgttgcacgg tgcaacagcg caatacg
//
LOCUS       DATBFZ010000141        11462 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_423730, whole genome shotgun sequence.
ACCESSION   DATBFZ010000141 DATBFZ010000000
VERSION     DATBFZ010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 11462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11462
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423730"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="VH419_04255"
     CDS             complement(<1..296)
                     /locus_tag="VH419_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEY7042864.1"
                     /translation="MLVAVTLTSCTGGNDEQNGSAQSEGPGTVTATLTGPTLTESAGG
                     NSGQPGPRRVRIAGTIASDLAVPWGLAFLPDGSALVTQRDAGTISAIDDGTVTQV"
     gene            complement(396..1994)
                     /locus_tag="VH419_04260"
     CDS             complement(396..1994)
                     /locus_tag="VH419_04260"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HEY7042865.1"
                     /translation="MGKRRSTPPQISLPGVVRRLGLGPAVLATAVATTGLTDGQHLGA
                     HAAAPAGQWAASGASDGSDTPQRRVTATSRPNVVLMVVDDATVEDIKYMPNVQSLLVD
                     HGTTFTRNYSPYPLCCPARATILTGQYSHNHRVLGNLPPLGGAPAFDPSHTIATYLDD
                     DYRTAMIGKYLNGFNTLTVPPGWDTFLTPIRATIYNYLDQAMNSNGSLQRFDNLYSTT
                     NYNDQALGFITSSQRPFFLDLSWVAPHNGSPLDTPTDPGGPYVAEPYRDTYDGPPLPD
                     DPSFNEADISDKRPSFQGHGSLSADRIAVIEDVLAQRREALRFVDDGVLSIVQAIADK
                     GQLGKTFFIFVSDNGWLQGQHRIATGKSSAYEPSSHVPLIIRGPGIPAGVEFDGLSGL
                     QDLTPTILDMTDEWAGKASTLIDGMSLLPLLEGTRTTDRPQVLEVATNAALTDQRADR
                     LALRWHGKARVTSVDWYMRGIVTADGWKYTEYPRTSEVEMYDLNSDPYEEHNLAGELL
                     HGKQQASLRALLRQYRSCKGLGCR"
     gene            complement(2052..2939)
                     /locus_tag="VH419_04265"
     CDS             complement(2052..2939)
                     /locus_tag="VH419_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY7042866.1"
                     /translation="MSLRFYPVASADGTRLNAWTNDADGPTVLLCNGLGTNAYAWPAL
                     LDDACTVRVVSWNHRGVGGSERPADPSRVGVDAFVEDALAVLDDAGIESCPVVGWSIG
                     VNTQFELATLFPERVTGMFCVAGVPGGTFASMLAPLMVPRFARRALTINVARSLRAVG
                     KVVSPISTRMPIGSASARMLRHSGFMTRRADNDVVRTALTEFMTTDVDWYMHLAVAAA
                     EHPRVTLTGIHVPTAFVAGRYDVLASARDMAAAAQQIERATYTELPGSHFVQMEHPDL
                     VHAELLHLLTRVAHAERRP"
     gene            complement(3004..3162)
                     /locus_tag="VH419_04270"
     CDS             complement(3004..3162)
                     /locus_tag="VH419_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042867.1"
                     /translation="MVRFWTWRPVSQAVALRNARHASAVLAQRRREREEIEAYLATLP
                     EQRKTLSA"
     gene            3345..4250
                     /locus_tag="VH419_04275"
     CDS             3345..4250
                     /locus_tag="VH419_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017935658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HEY7042868.1"
                     /translation="MKVFLPFPAEELDLPSSVTAHFEIEYADATEHWPDTAGECEFYV
                     PSYRFNRRVVEVMEDMPSLRVVQTLTAGVDHLLPYVPDGVTVCNAAGVHDAATSELAV
                     GLIIGAQRRLAELVRAQDRHIWDQQMAGALADRHVLVIGAGNIGRAIERRLAGFECSV
                     TLVGRTARHGVRAVAELPGLLPDADVVVLIIPITDETRGLVDREFLAPMKDGALLVNV
                     ARGPIVVTDDLLAEVSTGRLRAALDVTDPEPLPPDHPLWDASGVVITPHVGGASDAMW
                     PRSYRLVGQQLSRLAAGEPLLNQVG"
     gene            complement(4263..4784)
                     /locus_tag="VH419_04280"
     CDS             complement(4263..4784)
                     /locus_tag="VH419_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042869.1"
                     /translation="MRYLDIFRHTDNDGDALTDEGAAAAEAIGREKLSPPYAAFVSTG
                     AERATQMASILRSAAGQDDVPIGEQPALRSSVEDRWREASKAAGKGADLEAMRRVDPD
                     LVEKESQLLGAALRQVVTDTPDGARALVVGHSPTNEAAILGLAGVVVGPLGKGEGVTV
                     LESDGHYEVEPIS"
     gene            complement(4826..4966)
                     /locus_tag="VH419_04285"
     CDS             complement(4826..4966)
                     /locus_tag="VH419_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042870.1"
                     /translation="MRNLIRLAAGLALAASAVAIPVAAHAGIIFNGIDIANVGITMTA
                     LD"
     gene            complement(5134..5712)
                     /locus_tag="VH419_04290"
     CDS             complement(5134..5712)
                     /locus_tag="VH419_04290"
                     /inference="COORDINATES: protein motif:HMM:NF021886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEY7042871.1"
                     /translation="MTRQDLPAVPDLPTTAYALLGLLTLGDDLTGYELKRRADNTLRF
                     YWSSPAMSQVYTELARLAEHGLVAVREDETAPVRRTRRYRITATGRRDLARWLGQVET
                     DFPLLKHPIALRLLLGHLIEPDQTKAMLERYLDSLARRRQDLQTVRDSLGEEGDPERA
                     LYRYPRMVADWGLEYYESERVMVTELISRLDG"
     gene            5829..6890
                     /locus_tag="VH419_04295"
     CDS             5829..6890
                     /locus_tag="VH419_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017934122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HEY7042872.1"
                     /translation="MIGTRKGLWLARSDDDRKTWSLDGPHFNMEDVYSCHIDKRGGQV
                     RLYAGPASSWVGPKVARSDDLGQTWTEGKIAFPEDTGAALARIWQITPGLEESTVFAG
                     TEPGAVFRSDDRGDTFELVRGLWDHPHREQWGAGFGGQAFHTIVPHPTDSQAMTCAIS
                     TGGVYATEDRGATWEPHNTGVQAEFMPEGSQYPEFGQCVHKVARHPARPDRMFMQNHG
                     GVYRSDDGGVTWNSIEQGLPSNFGFPVVVHPHDPDTVYYFPLSGSGGRFPVDGKARVW
                     RSRDAGETWQALDDGLPDDFFVAVMRDAMCADDHEQAGIYFGARNGAVWASADEGDSW
                     QQVVANLPDVMCVRAATLD"
     gene            6961..8634
                     /gene="ilvD"
                     /locus_tag="VH419_04300"
     CDS             6961..8634
                     /gene="ilvD"
                     /locus_tag="VH419_04300"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007077403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEY7042873.1"
                     /translation="MVDIKPRSRVVTDGLEATAARGMLRAVGMGDDDFAKPQIGVASS
                     WNEITPCNLSLDRLAQAVKAGVHAAGGYPLEFGTISVSDGIAMGHEGMHFSLVSREVI
                     ADSVETVMMAERFDGSVLLAGCDKSLPGMLMAAARLDLAAVFLYAGSIMPGHLGDRDV
                     TIVDAFEAVGACLAGLIERDEVNRLERAICPGEGACGGMFTANTMASVAEALGMSLPG
                     SAAPPAVDRRRDGYAHKSGEAVVSMLRAGITARQVMTKPAFENAIAVVMALGGSTNAV
                     LHLLAIAREADVDLALDDFNRIGDKVPHLGDLKPFGRFVMNDVDRIGGIPVVMKTLVD
                     AGLMHGDCLTVTGKTMAENLDELSPPAVDGAIIREIAKPIHATGGLTILHGSLAPDGA
                     VVKTAGFDDDVVEGTARVFDGERAALDALAGGAITGGDVVVIRYEGPKGGPGMREMLA
                     ITGAIKGAGLGKDVLLLTDGRFSGGTTGLCVGHVAPEAVDAGPIAFVRDGDRIRLDVA
                     NRLLELLVDPAELDTRRVGWEPPPPKYPSGVLAKYRKLVQSAAVGAVCS"
     gene            8910..10664
                     /locus_tag="VH419_04305"
     CDS             8910..10664
                     /locus_tag="VH419_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017934112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HEY7042874.1"
                     /translation="MTNQAAPSMTGAQSLILALENAGVDTVFGIPGGAILPAYDPLLD
                     STKIRHILVRHEQGAGHAAEGYAAATRKVGVCMATSGPGATNLVTPLADAHMDSVPIV
                     AITGQVPSAAIGSDAFQEADIRGITMPITKHNYLITDADDIPRAIAEAFHIASTGRPG
                     PVLVDIAKDALQATTTFEYPDLINLPGYRPVTRPHGKQIREAARLIAAADRPVLYVGG
                     GVVRSGASEELRVLAELTRIPVVTTLMARGAFSDSHELHLGMPGMHGTVAAVGALQRS
                     DLLVSLGARFDDRVTGRLDTFAPDATIIHADIDPAEISKNRHADVPIVGDVREVIADL
                     VVAVQAEYDAGRRRDHSAWLATCKAWCDRYPLGYEQHPDSLSPQYVIERLGAIAGPET
                     TYVAGVGQHQMWAAHYVGYERPGSWINSGGLGTMGFAVPAAMGAKVGTPDRTVWAIDG
                     DGCFQMTNQELATCALNGIPIKVAVINNSSLGMVRQWQTLFYEGRYSNTDLHSVRVPD
                     FVKLAEALGCIGLRCDAAADVDGTIRKAMEINDAPVVVDFIVNPDAMVWPMVPAGTSN
                     DAIQIARDLKPEFDEDEL"
     gene            10678..11217
                     /gene="ilvN"
                     /locus_tag="VH419_04310"
     CDS             10678..11217
                     /gene="ilvN"
                     /locus_tag="VH419_04310"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEY7042875.1"
                     /translation="MTRQTLSVLVENKPGVLARIASLFARRGFNIESLAVGPTEHDDV
                     SRMTIVVNLEHQPLEQVTKQLNKLVEVIKVVELEGSAAVERELLLVKVRADSATRGQV
                     LETAQLFRAKVVDVAVEAVTIEVTGDRGKLEAMLRVLEPFGIRELVQSGMVAINRGSR
                     SITDRSLRPAPPISTRSPA"
     gene            11268..>11462
                     /locus_tag="VH419_04315"
     CDS             11268..>11462
                     /locus_tag="VH419_04315"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020661872.1"
                     /note="catalyzes the formation of
                     (R)-2,3-dihydroxy-3-methylbutanoate from
                     (S)-2-hydroxy-2-methyl-3-oxobutanoate in valine and
                     isoleucine biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEY7042876.1"
                     /translation="MAELFYDDDADLSVVQGRNVAVIGYGSQGHAHALSLRDSGVDVR
                     VGLPEASKSRPKAEAEGLRVV"
BASE COUNT         1830 a         3778 c         3951 g         1903 t
ORIGIN      
        1 acttgggtca ccgtgccgtc gtcgatcgcc gagatcgtcc ccgcgtcccg ctgcgtcacc
       61 agtgccgaac cgtcgggcag gaacgcgagt ccccacggca ccgcgaggtc gctggcgatc
      121 gtaccggcga tgcgtacccg tcgaggtccc ggttggcccg agttgccgcc ggccgactcg
      181 gtgagcgtcg ggccggtgag ggtcgcggtg accgtcccag gcccttcgga ctgcgccgag
      241 ccgttctgct cgtcgttgcc accagtgcac gaggtcaacg taacggcgac gagcatcatc
      301 gcgccggctc ggtatgacgg tcttgtcgcc ataccggatg gtctcatccg agtcaggccg
      361 agcatgaccc accggccggg acctggccgc aacggctacc gacagccgag ccccttgcag
      421 gatcggtact ggcgcagcag ggcccgcagg ctcgcctgct gcttgccgtg cagcagttcc
      481 ccagccaggt tgtgctcctc gtacggatcg gagttcaggt cgtacatctc gacctcactc
      541 gtcctcggat attcggtgta cttccaaccg tcggcggtca cgatcccgcg catgtaccag
      601 tcgacacttg tcaccctcgc cttgccgtgc cagcgcaggg ccagccgatc agcccgctga
      661 tccgtcagtg ccgcgtttgt ggccacctcc agcacctgcg gtcggtccgt cgtccgggtg
      721 ccttccaaca acgggagcag gctcatgccg tcgatcaggg tcgacgcctt acctgcccac
      781 tcatctgtca tgtcgagaat ggtcggtgtc aggtcttgaa gaccgctgag cccgtcgaac
      841 tcgacaccgg ccgggatgcc cggcccgcgg atgatgagcg ggacgtgtga tgacggctcg
      901 tacgccgagc tcttgcccgt cgcgatccgg tgctgaccct ggagccagcc gttgtccgag
      961 acgaagatga agaacgtctt gccgagctgt cccttgtcgg ctattgcctg cacgatcgag
     1021 agcacgccgt cgtcgacgaa acgaagcgct tcccgtcgct gggcgagcac gtcctcgatg
     1081 accgcgatcc ggtcggcaga cagtgacccg tggccctgga acgacggccg cttgtcggag
     1141 atgtcggcct cgttgaacga cgggtcgtcc ggcagcggcg gcccgtcgta cgtgtcgcgg
     1201 tacggctctg ccacgtacgg cccacccgga tcggtgggcg tgtcgagtgg agagccattg
     1261 tgcggggcga cccaggacag gtcgaggaag aacggccgtt gcgagctggt gatgaacccg
     1321 agggcctggt cgttgtagtt ggtcgtggag tagaggttgt cgaatcgttg gagcgatccg
     1381 ttggagttca tcgcctggtc gaggtagttg tagatggtcg ccctgatcgg ggtcaggaag
     1441 gtgtcccatc ccggcggaac cgtcaacgtg ttgaagccgt tcaggtactt gccgatcatc
     1501 gcggtccggt agtcgtcatc gagataggtg gcgatcgtgt gagacggatc gaaggcgggc
     1561 gctccgccga gcggtggcag gttgcccagc acccggtggt tgtgcgagta ctgaccggtc
     1621 aggatcgtcg ctcgggcggg gcagcacaac gggtacggcg aatagttccg cgtgaacgtc
     1681 gtcccgtggt cgacgagcag cgactgcacg ttgggcatgt acttgatgtc ctcgaccgtc
     1741 gcatcatcga cgaccatgag caccacgttg ggccgcgacg tggcagtcac cctccgctgg
     1801 ggtgtgtcag atccgtccga ggcaccggat gcagcccact gaccggctgg ggcggcggcg
     1861 tgcgccccga gatgctgccc gtcggtcaac ccggtcgtgg ccacggcggt cgcgagcacg
     1921 gccgggccca gtcccagcct gcgcacgacg ccgggcagcg agatttgtgg cggtgttgag
     1981 cgtcgcttcc ccatgattcc cccgatcgga cgacacacct cgcctgattg gaatacgagt
     2041 cgcaggtttc gtcaaggacg tcgttcggca tgtgcgacgc gggtgaggag gtggaggagc
     2101 tcggcgtgca cgagatcggg atgttccatc tggacgaagt gcgagcccgg cagttcggtg
     2161 tacgtcgcgc gctcgatctg ctgcgcagcg gcagccatgt cccgtgctga cgccagtacg
     2221 tcgtaccggc ccgcgacgaa cgctgtcggc acgtggatgc ccgtcagcgt gacgcgtgga
     2281 tgttcggcgg cggcgaccgc gaggtgcatg taccagtcga cgtcggtcgt catgaactcg
     2341 gtcagcgcgg tccggaccac gtcgttgtcg gcgcgccgcg tcatgaagcc gctgtggcgg
     2401 agcatccggg ctgaggcgct tccgatcggc atcctggtgg agatcgggga gacgaccttg
     2461 ccgaccgcgc ggaggctgcg tgcgacgttg atcgtgagtg cccgccgagc gaagcgcgga
     2521 accatcagtg gcgccagcat ggatgcgaag gtgccgccgg gtacgccggc cacgcagaac
     2581 attccggtca cccgttcggg gaacaacgtg gcgagctcga actgggtgtt gacaccgatc
     2641 gaccagccga ccaccggaca tgactcgatc ccggcgtcat cgagcacggc gagggcgtct
     2701 tcgacgaagg cgtccacacc cactcggctc gggtcggcgg gccgctcgga gccaccgaca
     2761 ccgcggtggt tccacgagac gacgcgcact gtgcacgcgt cgtcgagcag cgccggccag
     2821 gcgtaggcgt tcgtcccgag cccgttgcac agcagcacgg tcggcccgtc cgcgtcgttc
     2881 gtccacgcgt tcagccgggt cccgtctgca ctggccaccg ggtagaaccg caaggacatg
     2941 cggagattgt gccttgcaac tggtctcgcg gtcttcgccg ccgtgcaggc aggcggccgc
     3001 gtgtcaggcg ctcagtgtct tgcgctgctc ggggagcgtg gccaggtacg cctcgatctc
     3061 ctcacgctcg cgccgccgct gcgcaaggac ggcgctcgca tgtcgtgcgt tgcggagggc
     3121 gacggcctgg ctcaccggcc gccacgtcca gaatcgcacc atgaacccct ccttcacgtt
     3181 gttctgtcct ccgcgtgcca cgacgctagc cacgtcgtgc ttccgcgctg tgtcggctgt
     3241 gtgaattccg tgtgacgtgg attccgtgga ggagtcgctt actctagtaa gttactcaac
     3301 ttagtgtcaa ggcgcctgcg tgggcacgct ctagggttcg tgctgtgaag gtcttcctgc
     3361 cgttcccggc cgaagaactc gacctgccga gctcggtgac tgctcacttc gagatcgagt
     3421 atgccgacgc gaccgagcac tggccggaca ccgctggtga gtgcgagttc tacgtgccgt
     3481 cgtaccggtt caaccgtcgg gtcgtcgagg tgatggagga catgccgagt ctgcgcgtcg
     3541 tccagacact gaccgccggc gtcgaccatc tcctgccgta tgtaccggac ggcgtgacgg
     3601 tctgcaacgc tgccggcgtc cacgacgccg cgacgtctga gctcgccgtc ggcttgatca
     3661 tcggcgccca gcgtcggctg gccgagctgg tccgtgctca ggaccgccac atctgggacc
     3721 agcagatggc cggcgcgctc gcggacaggc atgtgctcgt gatcggtgcc ggcaacatcg
     3781 ggcgtgccat cgagcgcagg ttggcgggtt tcgagtgcag cgtcacgttg gtggggcgca
     3841 ccgcacgtca cggtgtccga gcggtcgccg agctgccggg gctgcttccg gacgccgatg
     3901 tggtcgtcct gatcatcccg atcaccgacg agacgcgtgg cctcgtggac cgtgagttcc
     3961 tggcccccat gaaggacgga gcgctgctgg tcaacgtggc gcgtggtccg atcgtggtca
     4021 ctgacgacct gcttgcggag gtgtcgaccg ggcggctccg cgcggcgctc gacgtcaccg
     4081 acccggaacc gctgccgccg gaccacccgc tgtgggacgc gtccggggtc gtcatcacac
     4141 cccacgtcgg cggtgcctcc gacgcgatgt ggccgcggtc ctaccggttg gtgggccagc
     4201 agttgagccg gctggcggca ggcgagccgt tgctcaacca ggtcggatga ctggccccca
     4261 cgtcagctga tcggctcgac ctcgtagtgg ccgtcggact cgaggaccgt gacaccctcg
     4321 cctttgccga gtggaccgac cacgaccccg gcgagaccca ggatcgctgc ttcgttcgtt
     4381 gggctgtgac cgacgaccaa cgctcgggcg ccgtcaggcg tgtccgtgac cacctgtcgg
     4441 agcgctgctc ccaacagctg tgactccttc tccaccagat cggggtcgac gcgtcgcatc
     4501 gcctcgaggt cggcgccctt gccggcggct ttggacgctt cccgccaccg gtcctcgacc
     4561 gacgaacgca gcgctggctg ctcgcctatc ggtacgtcgt cctgaccggc ggcgctgcga
     4621 aggatcgacg ccatctgggt cgcccgttcg gcgccggtcg agacgaaggc ggcgtacggc
     4681 ggactgagct tctcacgacc gatcgcctcc gcggcggcgg cgccttcgtc ggtgagcgcg
     4741 tcaccgtcgt tgtcggtgtg ccggaagatg tcgaggtacc gcatgaaccc ctcgtacccc
     4801 tgccggggaa ccgtatgcgg gcggatcagt cgagcgcggt catggtgatg cccacgttgg
     4861 cgatgtcgat cccgttgaaa ataatgccgg cgtgggcggc gactgggatg gcgaccgcgg
     4921 atgcagccag ggcgagaccg gcggccagtc ggatgaggtt gcgcatgacc tgctccttct
     4981 tccgaagctt tcaaagacac cagggattcg cggtcggccg cctatgggat tgccgcgctg
     5041 acgagaccgt ccggcgcccg ctggtcgagc ctgtcgagac cacagggcgg gtttcatgtc
     5101 cgcggccgcg gacgctcccg gccgccgaag aactcagccg tcgaggcggg agatcagctc
     5161 ggtgaccatg acgcgctcgg actcgtagta ctcgagtccc cagtccgcga ccattcgcgg
     5221 atagcggtac aacgcccgct ccggatcacc ttcctccccg agcgagtcgc gcacggtctg
     5281 cagatcctgc cgacggcggg cgagcgagtc gaggtaccgc tcgagcatgg cctttgtctg
     5341 gtcgggctcg atgaggtgac cgagcagcag ccgcagtgcg atcgggtgct tgagcagcgg
     5401 gaagtcggtc tccacctggc cgagccatcg ggcgaggtca cgtctgccag tggcggtgat
     5461 gcggtagcgg cgcgtccggc gtaccggcgc cgtctcgtcc tcacgcacgg caaccagacc
     5521 atgctcggcc aacctggcga gttcggtgta gacctggctc atcgcagggg aggaccagta
     5581 gaaccgcagt gtgttgtccg cgcggcgctt gagctcgtat cccgtcaggt cgtcgcccag
     5641 cgtcagcagc cccagcagcg cgtacgccgt ggtgggcagg tcgggaacgg ccggcaggtc
     5701 ttgacgcgtc atgatgcctg aacggtactc ttcctaatag gaatagtccg ttcgaagcga
     5761 ccgaccggac acgacgatct cgtgaccggc aggagggaac agccgccatg gcacgtcgag
     5821 cagtgatgat gatcggcacg cgaaagggcc tctggctcgc gcgctccgac gacgatcgga
     5881 agacgtggag tctcgacgga ccgcacttca acatggagga cgtctactcc tgccacatcg
     5941 acaagcgcgg cggccaggta cgcctgtatg caggtcctgc gtcgagctgg gtgggtccca
     6001 aggtcgcacg gtccgatgac ctggggcaga catggaccga aggcaagatc gcattcccag
     6061 aagacacggg tgcggcgctc gccaggatct ggcagatcac gccgggcctc gaggagtcga
     6121 ccgtcttcgc ggggaccgag cctggggcgg tcttccgctc cgacgaccgc ggcgacacct
     6181 tcgagctcgt gcgcggactg tgggaccacc ctcatcgtga gcaatgggga gccggtttcg
     6241 gcggtcaggc gtttcacacg atcgtgccgc accccacgga cagccaggcg atgacctgtg
     6301 cgatctcgac cgggggcgtc tacgcaaccg aggaccgcgg ggcgacctgg gagccgcaca
     6361 acaccggcgt acaggcggag ttcatgccgg agggcagcca gtacccggag ttcgggcaat
     6421 gcgtccacaa ggtcgctcgg catcctgcac ggccggaccg gatgttcatg cagaaccacg
     6481 gtggcgtcta tcgatccgac gacggcgggg tgacctggaa ctcgatcgag caaggtctgc
     6541 cgtccaactt cgggttcccg gtcgtcgtcc acccgcatga tccggacacg gtctactact
     6601 tccccttgtc cggatccggc ggccgtttcc cggtggacgg caaggccaga gtctggcgct
     6661 cccgcgatgc tggtgagacg tggcaggcgc tcgatgacgg gctgcccgac gacttcttcg
     6721 tcgccgtcat gcgcgacgcg atgtgcgccg acgaccacga gcaggccggc atctacttcg
     6781 gtgcacgcaa cggcgcggtg tgggcgtcgg ccgacgaggg cgactcctgg cagcaggtgg
     6841 tggccaacct gcccgacgtg atgtgcgtcc gcgccgcaac cctcgactga gttgtcagaa
     6901 gcccattgag ccagagccct accaggttct gacaactcgg ttggtggtta ggctgcggcc
     6961 atggtcgaca tcaagccgcg cagccgcgtc gtcaccgacg gactcgaagc cacggcggcg
     7021 cgcgggatgt tgcgcgcagt cggcatgggc gacgacgact tcgcgaagcc gcagatcggt
     7081 gtggcgtcgt cgtggaacga gatcacgccg tgcaacctgt cccttgaccg cctggcacag
     7141 gcggtcaagg ccggcgtgca cgcggccggc gggtacccgc tggagttcgg cacgatctcg
     7201 gtctccgacg gcatcgcgat gggccacgaa ggcatgcact tctcactcgt ctcacgagag
     7261 gtgatcgccg actcggtcga gaccgtgatg atggccgagc ggttcgacgg atcggtgttg
     7321 ctggccggtt gtgacaagag cctgcccggc atgctgatgg cggcagcgcg gctcgacctc
     7381 gctgcggtct tcctgtacgc cggctcgatc atgcccggcc acctcggcga ccgggacgtc
     7441 accatcgtgg acgccttcga ggccgtgggc gcctgcctcg caggactcat cgaacgcgac
     7501 gaggtgaacc gtctggagcg ggcgatctgc cctggcgagg gcgcctgcgg tggcatgttc
     7561 accgcgaaca cgatggcgtc ggtggccgag gcgttgggca tgtcgttgcc cggatccgct
     7621 gcgccacccg ccgtcgaccg tcgccgcgac gggtacgccc acaagtcggg agaagcggtg
     7681 gtcagcatgt tgcgggcagg catcaccgct cgtcaggtga tgacgaagcc ggccttcgag
     7741 aacgccatcg ccgtggtgat ggcgctcggc ggctcgacca atgcggtcct ccacctgctg
     7801 gcgatcgccc gcgaggccga cgtcgacctt gccctggacg acttcaaccg gatcggcgac
     7861 aaggtcccgc acctcggcga tctcaagccg ttcggccgct tcgtgatgaa cgacgtcgac
     7921 cggatcggcg ggatccctgt ggtgatgaag actctggtcg atgccgggct gatgcacggc
     7981 gactgcttga ccgtcaccgg caagaccatg gccgagaacc tcgacgagct gtcaccaccg
     8041 gccgtcgacg gagcgatcat ccgtgagatc gccaagccga tccacgccac cggcggactg
     8101 acgatcctgc acgggtccct cgcgccggac ggcgcggtcg tgaagacggc aggcttcgac
     8161 gacgacgtgg tcgagggcac ggcgcgtgtc ttcgacggtg agcgcgccgc cctcgatgca
     8221 ctggccggcg gtgcgatcac cggcggcgac gtcgtcgtca tccggtacga aggaccaaag
     8281 ggcgggccgg gcatgcgcga gatgctcgcc atcaccggcg cgatcaaggg tgccggcctg
     8341 ggcaaggacg tgctgctgct caccgacgga cgcttctccg gcggcacgac aggactgtgc
     8401 gtcggacacg tcgcgccgga ggctgtcgac gccggcccga tcgccttcgt ccgtgacggt
     8461 gaccggatcc gcctcgacgt cgccaaccgg ttgctcgagc tgctcgtcga cccggctgag
     8521 ctcgacactc ggcgggtcgg ctgggagcca cctccgccga agtaccccag cggtgtcctc
     8581 gcgaagtacc gcaagctcgt gcagtccgcc gccgtcggcg ccgtctgcag ctgaggcggt
     8641 cgccctcgct cgccgggcgc gtcttctgca gtggacgaac catggccgac gtatcccgga
     8701 cagaatgccc gaggggtgag acagatcagg gtcacgcttg accccgcccg tgcagtcgga
     8761 gacggtggtc tcgtgcagac gcttctcgtc gtacgcgtag gcgcgcaggc ccggacctga
     8821 cggtccccac ccgcgcgtcc ccctcgaatg ccttcgggcc gaggggtctt ttgttgccga
     8881 atcgaggacc ggtcaggaca ggaaggcaga tgacgaacca ggcggcgccg tcgatgacgg
     8941 gcgcacagag cttgatcctg gcgctggaga acgccggcgt cgacaccgtg ttcgggatcc
     9001 cgggcggcgc gatcctgccg gcgtacgacc cgcttctcga ctccacgaag atccgccaca
     9061 tcctggttcg gcacgagcag ggtgccggac acgcggccga gggctacgcc gcggccacgc
     9121 ggaaggtcgg cgtctgcatg gcgacgagtg ggccgggtgc caccaacctc gtgacaccgc
     9181 tcgctgacgc gcacatggat tcggtgccga tcgttgcgat cacggggcag gtgccgagcg
     9241 cggcgatcgg cagcgacgcc ttccaggagg ccgacatccg cggcatcacg atgccgatca
     9301 cgaagcacaa ctacctcatc accgatgccg acgacattcc gcgcgccatc gccgaggcgt
     9361 tccacatcgc gtccaccgga cgacctgggc cggtgctcgt cgacatcgcc aaggacgccc
     9421 tgcaggcgac aacgacgttc gagtacccgg acttgatcaa cttgcccggg taccggccgg
     9481 tgacacgccc gcacggcaaa cagattcgcg aagccgcccg gctgatcgcc gctgcagatc
     9541 gcccggtgct gtatgtcggg ggaggtgtcg tccggtcagg tgcgagcgag gagctacgag
     9601 tcctcgccga gctgacccgc atcccggtcg tcacgaccct catggccagg ggtgcgttct
     9661 cggacagcca cgagctccac ctgggaatgc ccggcatgca cgggacggtt gcggccgtcg
     9721 gcgcgctcca gcgcagcgac ctgctggtgt cgctgggagc gcgtttcgac gaccgggtga
     9781 cgggacggct cgacacgttc gcaccggacg ccaccatcat ccacgccgac atcgaccccg
     9841 cggagatctc gaagaaccgg catgccgacg tgccgatcgt gggtgacgtt cgcgaagtga
     9901 tcgccgacct cgtcgtcgct gtccaggcgg agtacgacgc aggacggcgg cgtgaccaca
     9961 gcgcctggct ggccacctgc aaggcgtggt gcgaccgcta tccgctcggg tacgagcagc
    10021 acccggactc gctgtcaccc cagtacgtga tcgagcggct gggcgcaatc gccggacctg
    10081 agacgacgta cgtggccggc gtcggccagc accagatgtg ggccgcgcac tatgtcggct
    10141 acgagcgacc cggctcgtgg atcaactccg gtgggctggg caccatgggg ttcgcggtcc
    10201 ccgccgcgat gggcgccaag gtcggtacgc cggaccggac cgtctgggcg atcgacggcg
    10261 acggctgctt ccagatgacc aaccaggagc tggccacgtg tgccctcaac ggcatcccga
    10321 tcaaggtggc ggtgatcaac aactccagcc tcggcatggt gcgccagtgg cagaccctgt
    10381 tctacgaggg caggtactcc aacaccgacc tgcactccgt gcgggtgccg gacttcgtca
    10441 agctcgccga ggcgctgggt tgcatcggcc tgcggtgcga cgcggcggct gacgtggacg
    10501 gcacgatacg caaagcgatg gagatcaacg acgcccccgt ggtggtcgac ttcatcgtca
    10561 acccggacgc gatggtgtgg cccatggtcc cggccggaac gagcaacgac gcgatccaga
    10621 tcgcgcggga cctcaagccg gagttcgacg aggacgagct gtgaagggcg attggtcatg
    10681 acgcggcaga cactttccgt cctggtggag aacaagcccg gtgtcctcgc caggatcgcc
    10741 tccctgttcg cccggcgcgg tttcaacatc gagtcgctcg ctgtcggacc gaccgagcac
    10801 gacgacgtct cccggatgac gatcgtcgtc aacctcgagc accagcctct cgagcaggtc
    10861 accaagcagc tcaacaagct cgtcgaggtg atcaaggtcg tcgagctgga ggggagtgcc
    10921 gcggtcgagc gggaactgct cctggtcaag gtgcgggccg actccgccac gcgtggccag
    10981 gtgctcgaga ccgcgcagct gttccgtgcg aaggtggtcg atgtggcagt cgaggcggtc
    11041 acgatcgagg tcacagggga tcgtgggaag ctcgaagcca tgctgcgggt gctcgagccg
    11101 ttcggcatca gggagctcgt gcagtccggg atggtggcga tcaaccgcgg ctcgcgctcg
    11161 atcaccgatc gaagcctgcg accggctccg cccatcagca cccgcagccc ggcctgaccg
    11221 gcccactcag ccccgcccgg cccgacgatc gaggagaagc accgaacatg gctgaactgt
    11281 tctacgacga cgacgccgac ctgagcgtcg tccagggccg caacgtcgcg gtgatcggct
    11341 acggcagtca gggtcacgcc cacgccttgt cgttacgtga ctcaggtgtc gacgtccggg
    11401 tcggtctgcc tgaggcgtcc aagagccgtc ccaaggcgga ggcggaaggg ctgcgcgtcg
    11461 tc
//
LOCUS       DATBFZ010000142         2498 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_4030383, whole genome shotgun sequence.
ACCESSION   DATBFZ010000142 DATBFZ010000000
VERSION     DATBFZ010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4030383"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..852
                     /locus_tag="VH419_04320"
     CDS             <1..852
                     /locus_tag="VH419_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020389912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY7042877.1"
                     /translation="ESGCGKSTLARALVGLVRPTSGEVRQDGRPLRYGDAGLKEFRRH
                     VQLVLQDPAGALNPRQNVYDAVAEGLRLHGLRAGLHEKVISALNRAGLRPPEQFFDRY
                     PHQLSGGQQQRVVIAGALVLDPDVIVADEPVSSLDASVRGEILKLILSLRDDLSLSAL
                     VVSHDLGLAWNIADRVAVMYLGRIVEIGSVTDVLLNPQHPYTKALIAAVPGGLGDPAL
                     AALPGEPPDPTRVPRGCRFHPRCPLRHSLDPADVRRERCLEDVQVLPVGGGEHLHACH
                     LVNDATR"
     gene            complement(922..2211)
                     /locus_tag="VH419_04325"
     CDS             complement(922..2211)
                     /locus_tag="VH419_04325"
                     /inference="COORDINATES: protein motif:HMM:NF024919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEY7042878.1"
                     /translation="MVSVVTSMVAVVGLTVEGSPVSSPSAEASTVRHAVTASKASVER
                     VALTRWVATRLRDGHFDAVRFARVKRGIVVGARPAHVTYRDPYGHTGRRSYERGRWTS
                     DWVRPAFAFERLIASYNASTPVGTFIEVAARARTTNGRIGSWDSLGRWASHDTGFHRM
                     SLGSQRDDVSRVAVDTLIAQGRLVAWQLRVSLFRRTTVTKTPVLRMVGAVASRIPERS
                     STSRPGPGVGHSLLVPRYSQEIHRGEYPRYNGGGEAWCSPTSTSMVLGYWHRLPPKRQ
                     YAWVDPSYADPWVDHAARFTYAWGYGGTGDWPFNTAYAARYGLDAFVTRLRSLREAEL
                     FIRAGIPLVATIAFGPGELDGAPIHSTSGHLVVIRGFTRSGRVIVNDPAAARNKGVRR
                     VYKRAQFENAWSDRGGVVYVIHPASKPLPAPRRHSNW"
     gene            2189..>2498
                     /locus_tag="VH419_04330"
     CDS             2189..>2498
                     /locus_tag="VH419_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002531175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042879.1"
                     /translation="MLVTTLTMLTMLTTLTTVELRIRLSVGSCRRLSLSKPREAFDFD
                     RLDQRLGGLDTLSRVTLRLYDTATREVRDFVPLEAGQVGIYHCGLTVQGPPHIGHIRK
                     E"
BASE COUNT          384 a          850 c          866 g          398 t
ORIGIN      
        1 gagtccggct gcggcaagag cacgttggcg cgggcgctcg tcggtctcgt ccggcccacg
       61 tcgggcgagg tcaggcagga cggccggccg ttgcgatacg gcgacgccgg tctgaaggag
      121 ttccgccggc atgtgcagct cgtcctgcag gaccccgccg gcgcgctcaa cccacggcag
      181 aacgtctacg acgcggtcgc ggaagggctg cggctgcacg gtctccgtgc cggtctgcac
      241 gagaaggtca tctcggcgct caaccgggcc ggacttcgtc cgcccgagca gttcttcgac
      301 cggtacccgc accagctgtc cggtggacaa cagcagcggg tggtgattgc tggcgcgctc
      361 gtcctggatc cagatgtgat cgtggccgac gagccggtgt cgtcgctgga cgcatcggtg
      421 cgcggcgaga tcctcaagct gatcttgtcc ctgcgcgacg acctctcgct ctcggcgctg
      481 gtcgtgagcc atgacttggg actcgcctgg aacatcgccg accgggttgc ggtgatgtac
      541 ctcggccgga tcgtcgagat cgggtcggtg accgacgtgc tgctgaaccc gcagcatccc
      601 tacacgaagg cgctgatcgc agcggtgccc ggaggcctcg gggatcccgc acttgccgcg
      661 ttgccggggg agcctcccga tcccacccgg gtcccgcgtg gatgccgatt ccatccgcgc
      721 tgcccgctgc gtcattcgct ggatccggcc gacgtacgcc gcgagcggtg tctcgaggac
      781 gttcaggtgc ttccggtggg tggtggtgag catctgcacg cctgccatct cgtcaacgac
      841 gcgacccgct gaccgaggtg gcaatcgagg tgcgacacgc cggcagaatc caacgaaacc
      901 tgccacgtcg gcgttggccg gctaccagtt cgagtgccgg cgaggagcgg ggagaggttt
      961 cgaggctggg tggatgacgt acacgacgcc gccgcggtcc gaccaggcgt tttcgaactg
     1021 tgcacgcttg tagacgcggc gcacgccctt gttccgtgct gccgccgggt cgttgacgat
     1081 cacccggccg ctgcgggtga agccgcggat caccacgaga tgaccgctgg tcgagtggat
     1141 cggtgccccg tcgagctcac cggggccgaa ggcgatcgtc gccaccaacg ggataccggc
     1201 gcggatgaac agctcggcct cgcgcaacga acgcagccgg gtcacgaacg cgtcgaggcc
     1261 atagcgtgcg gcatacgccg tgttgaaggg ccagtcgccg gtgccgccgt acccccacgc
     1321 atacgtgaag cgtgccgcat ggtcgaccca cggatcggcg tacgacgggt cgacccacgc
     1381 gtactgccgc ttcggtggca gtcggtgcca gtagcccagc accatcgagg tcgacgtcgg
     1441 gctgcaccat gcttccccac ctccgttgta gcgcgggtac tcgccgcggt ggatctcctg
     1501 cgagtaccgc ggcaccagca gcgagtggcc gacgccggga ccggggcggc tcgtcgagga
     1561 cctctccggg atccgcgaag cgactgcccc caccatccgc agcaccggag tcttcgtgac
     1621 cgtggtacgc cggaagagcg aaacccgcag ctgccacgcc accagccggc cctgcgcgat
     1681 cagcgtgtcg accgcgactc ggctgacgtc gtcgcgctgg ctgccgagcg acatccggtg
     1741 gaatcccgtg tcgtggctgg cccaacgacc cagcgagtcc caggacccga tccggccgtt
     1801 cgtcgtccgc gcgcgagcag ccacctcgat gaacgtgccg accggtgtgc tggcgttgta
     1861 cgaggcgatg aggcgctcga atgcgaacgc gggcctgacc cagtcgctgg tccagcggcc
     1921 acgctcgtac gaccgccggc cggtgtgacc gtacgggtcg cggtaggtca cgtgcgccgg
     1981 gcgggcgccg accacgatgc cgcgcttgac tcgggcgaag cggacggcgt cgaagtggcc
     2041 atctcgaagg cgtgtcgcta cccaccgtgt cagggccacg cgttcgacgg atgccttgga
     2101 ggccgtgaca gcgtgccgga ccgtcgaggc ttcagccgat ggcgagctga ccggtgatcc
     2161 ttcgacggtc agacccacga cagcaaccat gctggtgacc acgctgacca tgctgaccat
     2221 gctgaccacg ctgaccacgg tggagctccg cattcgcctc agcgtaggtt cctgccggcg
     2281 gttgagcttg tcgaaacccc gggaggcctt tgatttcgac aggctcgatc agcgactggg
     2341 cgggctcgat accctttctc gggtgacgct gcgcctgtac gacaccgcca cccgcgaggt
     2401 gcgcgacttc gtcccgctcg aggcgggcca ggtcgggatc taccactgcg ggctgacggt
     2461 gcaggggccg ccgcacatcg gccacatccg caaggagg
//
LOCUS       DATBFZ010000143         3610 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_1731313, whole genome shotgun sequence.
ACCESSION   DATBFZ010000143 DATBFZ010000000
VERSION     DATBFZ010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 3610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3610
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1731313"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(147..383)
                     /locus_tag="VH419_04335"
     CDS             complement(147..383)
                     /locus_tag="VH419_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042880.1"
                     /translation="MDPSKFIAPEFGSVHREPGNKWAFWYFKPALIPRDLLLTPATVS
                     VLSQADASLGRLQGIGALIRDPGVASARVVYGSR"
     gene            complement(776..863)
                     /locus_tag="VH419_04340"
     tRNA            complement(776..863)
                     /locus_tag="VH419_04340"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(827..829),aa:Ser,seq:gga)
     gene            944..1040
                     /gene="ffs"
                     /locus_tag="VH419_04345"
     ncRNA           944..1040
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VH419_04345"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(1059..1439)
                     /locus_tag="VH419_04350"
     CDS             complement(1059..1439)
                     /locus_tag="VH419_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY7042881.1"
                     /translation="MSNSVVHFEIPADDFERAQNFYREAFGWNMQHMPELQYTMVGTT
                     ESSEQGMPTNPGAINGGMGQRQSPHAEHPTVTIGVDDIDKTLERVSELGGSAVGEKNA
                     VGDMGWAAYFKDSEGNVIGLWQTA"
     gene            1633..3591
                     /locus_tag="VH419_04355"
     CDS             1633..3591
                     /locus_tag="VH419_04355"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011753812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma and tau"
                     /protein_id="HEY7042882.1"
                     /translation="MEAPLALYRRYRPDTFGDVIGQEHVTAPLSHALDNNRVHHAYLF
                     SGPRGCGKTTCARIMARALNCEKGPTSTPCGSCESCVDLARGGPGSIDVIEIDAASHG
                     GVDDARDLRERAMFSPMKYRYKIYIIDEAHMVSPQGFNALLKLVEEPPPHLRFIFATT
                     EPEKVIGTIKSRTHHYPFRLVPPKVLGDYLVELSDREGVAIEQTAVPLVVRAGAGSVR
                     DSLSVLDQLIGGSGPDGVTYDLATSLLGYTPDSLLDETVDALAADDGKTLFRVIDKVV
                     DAGQDPRRFAEDLLQRLRDLVVVSAVPDAASRGLIELSEDQAERLGGQAQRFGRAELT
                     RAADIVSTGLTDMRGATAPRLLLELMCARILLPGADQSVEGLGARIDRMERRLSVVGV
                     SHTASAVPTVPDHAEAGTPVAVPEGSTASPAPAEDAPAVAETAPAAAENAPSAADDAP
                     AAAVEPSQVVDASGRPSAGGLGLVDVRRLWPGVLDRVSTMKRMTWLLLSGSAQVRDLH
                     DGVLTIAFTEGARNNFLQSGHDEILRQALIDELGVAWTIEAIIDESIPTGRGGRGSSR
                     DRRPEPSPPTSTPASPPSEPTPPPSEAAKGAIRQTRAAGSKDRGGSEAPEVHDDDEVV
                     DDASLSGQELLARELGAQVIDEIRHDDR"
BASE COUNT          611 a         1208 c         1207 g          584 t
ORIGIN      
        1 gccacccacg gggagaactc ctgaccgcac atggggcgat cgcgatgacc gttgacaacg
       61 gtcatcgtgg tgtccttctt cgagtcggtt ttgcagatca ctcgaaggat cacccggtgg
      121 ccgtcgccta cgtcaacgcg acacgctcac cgggagccat acaccactct ggcggacgca
      181 actcccgggt cgcggatgag ggcaccgatt ccctgcagtc ggccaaggga ggcgtccgct
      241 tgagaaagca cactgacggt ggctggcgtg agcaagaggt cacgagggat cagagcgggc
      301 ttgaagtacc agaacgccca cttgttgccg ggttcgcggt gcaccgaccc gaactcgggt
      361 gcgatgaact tcgacggatc cacctggcga ccatatccca agtgaactgc cattttagtt
      421 tgaacttagg ttcgacgctc cgagtcgact tggggcctcg aggatcgtga acgacatcac
      481 aagcctccaa agaatcttgg tgaagaacag ccgtgcgacg agacttggag cgtcgtccaa
      541 acgctccaag cactcagtct tcggagttgg atcttctgtg ccggagaggc tctcggtgag
      601 gcattcggtg aggccgttcc gacggtccgg tgaggaccgt ttgtcaccat cgggcggacg
      661 gcgacgcgag ccaactgaga ccagaagtga gcccgcagcc cctcctcggc gacgggatgg
      721 gtctttggcc gacggcggcg ccgtggcgtt ctcgcaggtc aacacgcatg ctcagtggcg
      781 gaggataggg gattcgaacc cctgagggcg ttaacccaac acgctttcca agcgtgcgca
      841 ctaggccact atgcgaatcc tccgtgcagg agggtatcgc ctggcgcggg gttgtccgaa
      901 tcgagcacga attggagcag gacccgtgct cccgtagact cggcgcagcc cctcgtgtgg
      961 cggcatctcg cccaactccc ccagggccgg aaggcagcaa gggtaagtga gctctgccgg
     1021 gtacacgagg ggtcttcgcg tcccagcccg cgggtgtgtc aggcggtctg ccagaggccg
     1081 atcacgttgc cttcgctgtc cttgaagtac gccgcccagc ccatgtcgcc gacggcattc
     1141 ttctcgccca ccgccgagcc gccgagctcg ctcacccgct ccagcgtctt gtcgatgtcg
     1201 tcgacgccga tcgtgaccgt cgggtgctct gcatgcggtg actgccgctg gcccatcccg
     1261 ccgttgatgg cgccgggatt ggtcggcatg ccctgttcgc tgctctcggt ggtcccgacc
     1321 atcgtgtact gcagctcggg catgtgctgc atgttccagc cgaaggcttc gcggtagaag
     1381 ttctgcgccc gctcgaagtc gtcagccggg atctcgaaat ggacgacgct gttgctcatg
     1441 ctggctcctc gtctcgtcga agatcgactc ctctcgcgag cctaggtcgc ggatccgaca
     1501 acgtcacccc tcgctgcgaa ttgcgggaaa gaaccggccg acacgccgag tggaaggcgc
     1561 ggtttcccgc aattcgcggg agggacggag caaccgcgga ccgtgtcgtc gtacggcgct
     1621 agggtcgtgc ccgtggaagc ccctctcgcg ctgtaccggc ggtaccggcc ggacaccttc
     1681 ggcgacgtga tcggtcagga gcacgtcacg gcgccgctgt cgcacgcgct cgacaacaac
     1741 cgggtgcacc acgcctacct gttctccgga ccgcgcggct gcggcaagac cacgtgtgct
     1801 cggatcatgg cgagggcgct gaactgcgag aagggcccga cctccacgcc gtgtggcagc
     1861 tgcgagtcct gcgtcgacct ggcgcgaggc ggccccggct cgatcgacgt gatcgaaatc
     1921 gacgcggcca gccacggagg tgtcgacgac gcccgtgacc tgcgcgaacg cgcgatgttc
     1981 tcgccgatga agtaccgcta caagatctac atcatcgacg aggcacacat ggtctcccca
     2041 caaggcttca acgctctgct gaagctcgtg gaggagccac cgccacacct gcgtttcatc
     2101 ttcgcgacca ccgagccgga gaaggtcatc ggcacgatca agtcgcgcac acaccactac
     2161 ccgttccggc tggtgccgcc gaaagtcctc ggcgactacc tcgtcgagct cagcgatcgc
     2221 gaaggggtgg cgatcgagca gaccgccgtg ccgctggtgg tccgggcggg ggcgggctcc
     2281 gtcagggaca gcctctcggt gctcgaccag ctgatcggtg gatctggtcc ggacggtgtc
     2341 acgtacgacc tggcgacctc cttgctcggc tacacgcccg acagcctgct cgacgagacc
     2401 gtcgacgcgc tggcggccga cgacgggaag acgctgttcc gggtgatcga caaggtcgtc
     2461 gacgccggcc aggatccacg ccggttcgcc gaagacctgt tgcagcggct gcgcgacctc
     2521 gtcgtcgtct ccgcggttcc cgatgccgca tcccgtggcc tgatcgagct ctcggaggac
     2581 caggccgagc ggctcggcgg gcaggcgcag cggttcggtc gggccgagct caccagagcg
     2641 gccgacatcg tctccacggg tctcaccgac atgcggggcg cgaccgcgcc gcggctgctg
     2701 ctcgagctga tgtgcgcccg catcctgctt ccgggcgctg accagagtgt cgagggcctc
     2761 ggtgcgcgga tcgatcggat ggagcggagg ctctcagtgg tcggggtttc ccacaccgct
     2821 tccgcagttc ccacggtgcc ggaccatgct gaagccggta cgccggtcgc tgtccccgag
     2881 ggatcgactg cctcgccggc ccccgccgaa gacgcgccgg ccgtagccga gacagcgccg
     2941 gccgcagccg agaatgcgcc gtccgccgcc gacgacgccc cggccgcggc ggtcgaaccg
     3001 tcgcaggtcg tcgacgcctc ggggaggcct tcggccggcg gactcgggct cgtcgacgtc
     3061 cgcagactgt ggcccggcgt cctcgaccgt gtcagcacga tgaagcggat gacgtggctg
     3121 ctgctgtccg gcagcgccca ggtgcgcgac ctgcatgacg gggtcctgac gatcgcgttc
     3181 accgagggcg cccgcaacaa ctttctccag agcgggcacg acgagatcct gcgtcaggca
     3241 ctgatcgacg agctcggtgt ggcgtggacg atcgaagcca tcatcgacga gtcgattccg
     3301 accggtcgag gcgggcgcgg gtcgagccgc gatcgacgac cagagccgtc cccacccacg
     3361 agtacgccgg cctcgccacc gagcgaaccg acaccgcctc catccgaggc tgcaaagggc
     3421 gcgatccgtc agacgcgcgc ggcaggcagc aaggatcgcg gcgggtccga ggcgcccgag
     3481 gtccacgacg acgacgaggt cgtggacgac gcgtcgctga gcggccagga gctgctcgcc
     3541 cgtgagctcg gcgcgcaggt gatcgacgag atccgacacg acgaccgcta ggaccaagga
     3601 gctgagcgat
//
LOCUS       DATBFZ010000144         3353 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_5955108, whole genome shotgun sequence.
ACCESSION   DATBFZ010000144 DATBFZ010000000
VERSION     DATBFZ010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 3353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3353
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5955108"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..793)
                     /locus_tag="VH419_04360"
     CDS             complement(<1..793)
                     /locus_tag="VH419_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007033950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase family protein"
                     /protein_id="HEY7042883.1"
                     /translation="MSPFRGSAPFTEEWKHFDVLRESGVATVTFNRPDKLNALTFEAY
                     ADLRDLFAELPHREDTRVVVLRGDGRAFCSGGDVDEIIGATLAMRPDELLSFTRMTGE
                     VVRAMRESPVPVVASIQGVAAGAGSVLALASDFRVVARTATFAFLFTKVGLAGADMGA
                     AYLLPRLVGLGRATQALMLGDPLDAETADDWGLVTELVDEEVLDETSHALATRLATGP
                     WQAYSATKSLLSRELDLGLSGAVELEAMTQALLMKGDDYAEFHAAF"
     gene            1012..>3353
                     /locus_tag="VH419_04365"
     CDS             1012..>3353
                     /locus_tag="VH419_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017936274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003959 - NADPH dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional salicylyl-CoA
                     5-hydroxylase/oxidoreductase"
                     /protein_id="HEY7042884.1"
                     /translation="MPLRIAVIGGGPGGLYFAALAKQLGPDHEITVWERNASDDTFGF
                     GVVFSDETLGGIEHADPPIHAAMEAEFARWDDIDVHFGGEVITSGGHGFAAMSRRRLL
                     EILQGRCRELGVDVRFRTEAPPVEDLAAAYDLVVASDGLNSPVRTAYAESFQPTIETR
                     KCRYMWLGTDKVFDAFTFVVRETPYGVMQIHAYPYDAHGSTFIIEMHDDVWERAGFAD
                     LAPTDLRPGESDEKSIARVCELFADVLDPSTGSGQAAVIGNNSKWVSFATIRNASWVH
                     ENVVLLGDAAHTAHFSIGSGTKLAMEDALALAACLHEVPALPDALSAYELERRPVVLS
                     TQRAAQASLEWFENLGQYTHQEPMQFAFNIMTRSRRVTYDNLRVRDHEFVERVDHWFA
                     DHEKRRGVGDGDVRPPMFHPFRLRDLTLANRVVVSAMDMYMADDGVPGDFHFTHLTGK
                     ALGGAGLVMTEMTCVSPEGRITPACCGLYDDRQTTAFRRITDFVHEQTDAAVGIQLGH
                     SGRKGSTKRMWEGMDEPLPDGNWEVVAPSPVAYKPGVNQVPRELTRGDMASIRAEFVA
                     AARRAEAADFDLLELHCAHGYLLSSFISPLTNRRTDSYGGSLENRLRYPLEVFESIRD
                     VWPSGKPMTVRISATDWAEGGIDTEDAVEVARAFAAVGADAIDVSTGQVTPEERPDFG
                     RSYQTPYADAIRNRVDIATIAVGVISSYDDVNSLVLAGRADLCALARVHLYDPNWTLH
                     AAIEQDYAGPGVRWPDPWLPGRRKPQTGRTDGPKPRLQLIR"
BASE COUNT          539 a         1127 c         1179 g          508 t
ORIGIN      
        1 tgaacgcggc gtggaactcg gcgtagtcgt cgcccttcat caacagcgcc tgcgtcatcg
       61 cctcgagctc gaccgcaccg gagaggccaa ggtcgagctc gcggctgagc agcgacttcg
      121 tggcggagta cgcctgccac ggcccggtgg ccagacgcgt cgccagcgcg tggctggtct
      181 cgtcgagcac ctcctcgtcg acgagctcgg tgacgagtcc ccagtcgtcg gcggtctcgg
      241 cgtcgagcgg gtcgccgagc atcagggcct gcgttgcgcg gccgaggccg acaagccggg
      301 gaagaaggta cgccgccccc atgtccgcac cggccagccc gaccttcgtg aagaggaagg
      361 cgaacgtcgc cgtgcgggcc acgacgcgga agtccgaggc gagcgccagc accgaaccgg
      421 cgccggccgc cacaccttgg atcgaggcga cgacgggaac cggtgactcg cgcatcgccc
      481 ggaccacctc gccggtcatc cgggtgaacg acaacagctc gtcggggcgc atcgccaggg
      541 tcgcgccgat gatctcgtcg acgtcgccgc cggagcagaa cgcccggccg tcaccacgca
      601 ggacgacgac ccgcgtgtcc tcgcgatgcg ggagctcggc aaacaggtcc cgcaggtcgg
      661 cgtacgcctc gaacgtcagg gcgttcagct tgtcgggccg gttgaacgtg acggttgcga
      721 ctccggactc acgcagcacg tcgaagtgtt tccactcctc cgtgaacggc gcggatcccc
      781 ggaacgggct cattcggcgg caacctcctt gttggtccgg tgctgcgacc ttgatctcga
      841 caagctcgat cagcggggtc cggcggtcag cggtctgggt acggctcagc gtagccagcc
      901 ggccgaatcg tgtgtgccgg gagtggcgtt cgattgacgg tcgttgtcgc gacgacatac
      961 gctcgaaggc cacgacatcg ccgacgatgc cagtccgacc ctcctcagga gatgcctttg
     1021 cggatcgctg tgataggtgg cgggccgggc ggactgtact tcgccgcgct ggcgaaacag
     1081 ctcggcccgg accacgagat caccgtgtgg gagcgcaacg ccagcgacga cacgttcggc
     1141 ttcggagtcg tcttctccga cgagacgctc ggcgggatcg agcacgccga cccgccgatc
     1201 catgccgcga tggaggccga gttcgcccgc tgggacgaca tcgacgtgca cttcggcggc
     1261 gaggtgatca ccagcggtgg ccacgggttc gccgcgatga gccgtcgccg cctgctcgag
     1321 atcctgcagg ggcgctgccg ggagctcggt gtggacgtgc gtttccgcac ggaggcgcca
     1381 cccgtcgagg acctcgccgc ggcgtacgac ctcgtcgtcg ccagcgacgg cctcaactcg
     1441 ccggttcgga cggcgtacgc cgagtcgttc cagccgacga tcgagacccg gaagtgccgc
     1501 tacatgtggc tcggcaccga caaggtgttc gacgcgttca cgttcgttgt ccgcgagacg
     1561 ccgtacggcg tgatgcagat ccatgcctat ccgtacgacg cccacggcag cacgttcatc
     1621 atcgagatgc acgacgacgt gtgggagcgg gcaggtttcg ccgaccttgc accgacagac
     1681 ctgcggcccg gcgagtccga cgagaagtcg atcgcccggg tgtgtgagct gttcgccgac
     1741 gtgctcgacc cttcgacagg ctcagggcaa gcggccgtca tcggcaacaa ctcgaagtgg
     1801 gtcagcttcg ccaccatccg caacgcgtcc tgggtgcacg agaacgtcgt cctgctcggc
     1861 gacgcggcgc acaccgcgca cttctcgatc gggtccggca cgaagttggc gatggaggac
     1921 gctttggcgt tggcggcctg cctgcacgag gtaccggcgt tgccggatgc gctgtcggcg
     1981 tacgagctcg aacgacgtcc ggtggtcctc tcgacccaac gagccgcgca ggcgagcctg
     2041 gagtggttcg aaaacctcgg gcagtacacg caccaggagc cgatgcagtt cgcgttcaac
     2101 atcatgacgc gcagccgccg ggtgacgtac gacaacctgc gggtgcgtga ccacgagttc
     2161 gtcgagcgcg tcgaccactg gttcgccgac cacgagaagc gtcgaggtgt cggcgacggg
     2221 gatgtccggc cgccgatgtt ccacccgttc cggctgcgtg acctcacgct ggccaaccgg
     2281 gtggtggtgt cggcgatgga catgtacatg gcggacgacg gcgtccccgg cgacttccac
     2341 ttcacccacc tcaccgggaa ggcgctcggc ggggcgggtc tggtgatgac cgagatgacg
     2401 tgtgtgtctc cggaggggcg catcacgccg gcctgctgcg gcctgtacga cgaccggcag
     2461 acgacggcgt tccggcggat caccgacttc gtgcacgagc agacggatgc cgccgtgggg
     2521 atccagctcg gccactcggg gcgcaagggc tcgacgaagc ggatgtggga ggggatggac
     2581 gagccgcttc ctgacggcaa ctgggaagtg gtggcgccgt cacctgtcgc ctacaagccc
     2641 ggcgtcaacc aggtgccccg cgagctgacg cggggcgaca tggcgagcat ccgcgcggag
     2701 ttcgtggcgg cggcgcgacg ggcggaggcg gccgacttcg acctgctgga gctgcactgt
     2761 gctcacggat acctgctgtc gagcttcatc tcgccgctga ccaaccggcg tacggactcc
     2821 tacggcggat ccctcgagaa ccgcctccgc tacccgctcg aggtgttcga gtcgatccgc
     2881 gacgtgtggc cgagtgggaa gccgatgacg gtgcggatct cggcgaccga ctgggctgag
     2941 ggtgggatcg acaccgagga cgcggtcgag gtggcgcgcg cgttcgcggc cgtcggcgcc
     3001 gacgcgatcg acgtgtccac cggacaggtc acgccggagg agcggccgga cttcggacgg
     3061 tcgtaccaga cgccgtacgc cgacgcgatc cgcaaccgcg tcgacatcgc gaccatcgcg
     3121 gtgggcgtga tctcgtcgta cgacgacgtc aactcgctcg tcctcgccgg ccgggccgac
     3181 ctgtgtgcgc tggcacgggt ccacctgtac gacccgaact ggacgttgca cgccgcgatc
     3241 gagcaggact acgccggtcc gggtgtgcgc tggccggacc cgtggctacc ggggcggcgc
     3301 aaaccgcaga ccggccggac cgacggcccc aaaccgcgcc tgcagctcat ccg
//
LOCUS       DATBFZ010000145         7932 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_2063760, whole genome shotgun sequence.
ACCESSION   DATBFZ010000145 DATBFZ010000000
VERSION     DATBFZ010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 7932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7932
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2063760"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="VH419_04370"
     CDS             <1..363
                     /locus_tag="VH419_04370"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEY7042885.1"
                     /translation="AGAHRHDRRVGCLVQVSLDEAPEQAGRGGVEPAGIDEVADAIDS
                     AGMLDLMGVMGVAPLRGNAAAAYERLLDVSRRLQESHPGARLVSAGMTADFETAIQAG
                     ATHVRVGSAVLGERPENE"
     gene            453..956
                     /gene="sepF"
                     /locus_tag="VH419_04375"
     CDS             453..956
                     /gene="sepF"
                     /locus_tag="VH419_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020137459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEY7042886.1"
                     /translation="MAGAMRKVGVYLGLLEDTDRYEDDYYADDERHDAAQAAPAMRPV
                     HAVRDDRPAKETRDTTVASLSERRRPTQVAELSRITTLHPRNYNEARTIGEHFREGIP
                     VIMNLSEMDDSDAKRLVDFSAGLVFATRGSIERVTSKVFLLSPPNVSVAAEDKQRIAE
                     GGFFNQS"
     gene            1031..1327
                     /locus_tag="VH419_04380"
     CDS             1031..1327
                     /locus_tag="VH419_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEY7042887.1"
                     /translation="MVFVGVVIELILWIYILLVLSRLVADWVQMFARSWSPHGPVLVL
                     MELIYSTTDPPIRLFRRFVPPLRLGGAALDLSVIFVLLVCYVLYYVNRKVLLFG"
     gene            1491..2201
                     /locus_tag="VH419_04385"
     CDS             1491..2201
                     /locus_tag="VH419_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEY7042888.1"
                     /translation="MPLTPEDVNKKTFTPVRLREGYDMGEVDQFLDEVETALTQLQEE
                     NDDLRQKLSAATAAPPQQAGQAEPAKEPVREEVVPSPLPAVSTVPEASSAAARLLEIA
                     AQNADQLVNEAKDEADKILGEARSKSERLEADSKSKADRLESDAQTRAEKLDSETSER
                     RAQLFGQLERDKDNLARELEDLRAFEREYRSRLKGYFQAQLRALDGEGDGEVPLTPSA
                     EGETPRRLRELLGEEQQD"
     gene            complement(2381..5596)
                     /gene="ileS"
                     /locus_tag="VH419_04390"
     CDS             complement(2381..5596)
                     /gene="ileS"
                     /locus_tag="VH419_04390"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007078063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEY7042889.1"
                     /translation="MSTDTAPASSGGSYQPVPPQVDLPAMEREILRFWREHDTFRRSL
                     EQTAEGPRWTFYEGPPTANGKPGTHHVEARVFKDVFPRFKTMRGFRVDRKAGWDCQGL
                     HVEVGVEKEIGVSGKQQIEAYGIAEFNAKCRESVERSTDDFEEMTERMAYWVDMSQAY
                     RTMDRTYIESVWWSLKQVFEKGLLYESHRVTPYCPRCETALSDHELGQPGGYETVVDP
                     SIYVRFPLTSGPYAGQASLLVWTTTPWTLVSNTAVAVHPDVEYVVASDGSETLIVAEP
                     LFQSVLGDGWTVTDRRAGRDMEYWRYQRPLDLVDFPTDDPSEDDDPSHDRGDAGAQPR
                     AHHVVLADYVTTEDGTGLVHQSPAFGGEDMRVTQGYGMPVVNPIRRDGHFEDQVRLVG
                     GQFFKHADADLVRELESRGLMFRHVAHEHSYPHCWRCHTPLMYYALPAWYIRTTAIKD
                     ALLRENEKTNWYPETIKWGRYGDWLRNNVDWSLSRTRYWGTPLPIWRCGDDHLTCVGS
                     LAELGELAGRDLNDLDPHRPYVDDVVLPCPECGAEARRVTEVIDAWYDSGSMPFAQWG
                     YPYVDGADERFHEAFPAQFICEAIDQTRGWFYSLMAIGTLLFDETSYENVVCLGLLLA
                     EDGRKMSKHLGNILEPMPLMEQHGADAVRWFMCASGSPWAARRVGNATLQEIVRKVLL
                     TYWNTVAFQVLYARTNGWTPDQSAPAPDRRPVLDRWLLSQVHRLTRDVTLALEDFDTQ
                     RVGTQLAEFVDELSNWYVRRSRRRFWDGDPAALATLHETLHVLTRLLAPITPFVTERV
                     WQDLFRRTDGTADSVHLATWPTYDEELIDDRLDEGMSLARRLVELGRSARAEAKVKTR
                     QPLQRALIPSTAYAELSDELREEVAAELNVLRVESFATAGDLVDHSAKANFRTLGKRF
                     GKQTPMVAAAIAGADASALAERLSTAGRATVDFDGGVEVTADEVVLSERPREGWSVVN
                     EHGETIALDVHLTPELVRSGLAREFVRLVQEARKSSDYDVSDRIVLTWSGEGDAAEAL
                     VEHRDLVAREVLAVRTERVDAPGDDWWRDDDLKLAFIVEKA"
     gene            6038..6877
                     /locus_tag="VH419_04395"
     CDS             6038..6877
                     /locus_tag="VH419_04395"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HEY7042890.1"
                     /translation="MAARSTSAIKAVKQAVGKATRRGGGSAAPKAASAKTATPAKKAA
                     SAKKTATAKKAAPAKKTAAAKKAAPAKKAAPAKKAPPAKTAAPAKKTAAAKKTAAAKK
                     AAPAKKLSASKAAPATRTSPGSPPARKAAALATDIGAAPAPAKKVRGGKLAVREDEKP
                     WTAGEIKEVRANLEHDATRLKAEIAATEEEIGHLLREGGEGAGNDQADVGSNTFERDH
                     EMTMAKNARESLHVIEAALRRIDDGTYGICESCGRPIGKMRLQAFPRATLCMECKQRQ
                     ERR"
     gene            6850..7425
                     /gene="lspA"
                     /locus_tag="VH419_04400"
     CDS             6850..7425
                     /gene="lspA"
                     /locus_tag="VH419_04400"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEY7042891.1"
                     /translation="MQTTPGTSLTDDTPTTPPPARHHRILLWAAVAAFAYLLDQATKT
                     WASHTLEDGQSRAIIGDLLTFHLTHNPGAAFSMGTGYTVVLTVIALTVIVVCIRMAGR
                     LGSTGWAIALGLLLGGALGNVTDRIFRAPAPFRGHVVDFIEIPHWPVFNFADTAISIA
                     AVLFVLQSARGVHLDGRVERRRDLDDEPTEP"
     gene            7518..>7932
                     /locus_tag="VH419_04405"
     CDS             7518..>7932
                     /locus_tag="VH419_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014350147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEY7042892.1"
                     /translation="MFGLSRTKAAELVAAGDVTLDGVRPAKSDRVEEGAFLEVLIPAP
                     DPGVRVRPEVVAGLRIMYDDQSFVVVDKPVGVAAHPSVGWTGPTVLGHLAGAGFRIAT
                     SGVPERQGIVQRLDVGTSGLMVVAKSEYAYSRLKRA"
BASE COUNT         1378 a         2683 c         2663 g         1208 t
ORIGIN      
        1 gccggcgcgc atcggcacga ccgccgggtt ggctgcctcg tccaggtcag cctcgacgag
       61 gcgcccgagc aggcgggacg aggcggggtc gaacccgccg ggatcgacga ggtcgccgac
      121 gcgatcgaca gcgcaggaat gctcgacctc atgggtgtga tgggcgtggc tccgttacgc
      181 gggaacgccg cggcggcgta cgaaaggctg ctggacgtca gccggcggct ccaggaaagt
      241 caccccggcg cgaggctcgt ctcagcaggg atgacagcag atttcgagac cgcgatccag
      301 gccggagcga cacacgtgcg tgtcggcagc gcggtgctcg gtgagcgacc ggagaacgag
      361 tagtgtcaag tcaggcggcc gctggagagc tcaggcagca ggtgacagcg atccggcaac
      421 tgcgtcaacg atgaggtgga ggaaccgaag tcatggctgg tgcaatgcgc aaggtcgggg
      481 tctacctggg cctgctggaa gacaccgacc gctacgagga cgactactac gccgacgacg
      541 aacgtcatga cgcggcccag gccgcaccgg ccatgcgtcc cgtccacgcg gtgcgtgacg
      601 accgacctgc caaagagacg agggacacca ctgtcgcatc gctgtccgag cgtcgtcggc
      661 ccacgcaggt cgcggagctg tcgaggatca ccacgctgca cccgcgcaac tacaacgaag
      721 cgcggaccat cggggagcac ttccgcgaag ggatcccggt gatcatgaac ctgtcggaga
      781 tggacgacag cgacgccaag cggctggtcg acttctccgc cggcctggtc ttcgccaccc
      841 gcggcagcat cgaacgcgtg accagcaagg tcttcctgct ctcgccgccc aacgtctcgg
      901 tcgccgccga agacaagcag cggatcgccg aaggcggctt cttcaaccag tcgtaggacc
      961 ggcggttgag cttgtcaaaa ccccttcgac aggctcaatc ggctgagtgc gcaccatcta
     1021 ggcttgtgac gtggtcttcg tcggcgtcgt catcgagctc atcttgtgga tctacatcct
     1081 gctcgtcctg tcgcgcctgg tcgcggactg ggtacagatg ttcgcccgct cgtggagtcc
     1141 acacggtccc gtgctggtgc tgatggagtt gatctactcg acaacggatc cgccgatccg
     1201 gctcttccgg cgctttgtcc cgccgctgcg actgggcggg gccgcgctcg acctctcggt
     1261 cattttcgtg ctgctggttt gctacgttct ctactacgtg aaccggaagg tcctgctgtt
     1321 cggttaggct gagcgagccg ttcatcgctg gactcaggac cgggttcgaa gccacttcga
     1381 ccatcccatc gcggcgatgt ccgcacgtga ggtcgcgccg tacaacgcac cgaccaggga
     1441 gtacggtggc atcgcacgac cgaccagatc aggactactg aggtgagctc atgccgttga
     1501 cgcccgagga cgtaaacaag aagacgttca cgcccgttcg cctgcgtgag ggatacgaca
     1561 tgggggaggt cgaccagttc ctcgatgagg tcgagaccgc gctcacccag ctgcaggagg
     1621 agaacgacga cctccgccag aagctgtcgg ctgcgacggc tgcgccgccc cagcaggccg
     1681 gtcaggccga gccggcgaag gagccggtgc gcgaagaagt cgtcccgtct ccactgccgg
     1741 ccgtcagcac cgtgcccgag gcatcgagtg cggcggcgcg gctgctcgag atcgccgcgc
     1801 agaacgccga ccagctggtc aacgaggcca aggacgaggc cgacaagatt ctcggcgagg
     1861 cccgcagcaa gtccgagcgg ctcgaggctg actcgaagtc caaggcggac cggttggagt
     1921 ccgacgccca gacccgcgcc gagaagctcg actccgagac gtcggagcga cgagcccaac
     1981 tgttcggtca gctcgagcgc gacaaggaca acctcgcccg cgagctcgag gacctgcgcg
     2041 cgttcgaacg cgagtaccgc agcaggctga agggctactt ccaggcacag ctgcgggcgc
     2101 tcgacggaga aggcgacgga gaagtcccgt tgacgccatc agcggagggc gagacaccgc
     2161 gccggctgcg cgagctcctc ggcgaggagc agcaggactg atcctggcga ccttcaccac
     2221 cattcgaatt cggacacacg tcctgcggtg gttttcctgg gtagccgtgc aaacggacgg
     2281 ctacccaggc aaacctgcct cgggaggcgt ggggctgcct gggtagccat gcaaaccgcc
     2341 gccaccatcg caagacctcc agcgtgggcg gcgacgcctg tcacgccttc tcgacgatga
     2401 acgccagctt caggtcgtcg tcgcgccacc agtcgtctcc gggcgcatcg acccgctcgg
     2461 tacggacggc cagcacttcc cgggccacga ggtcacgatg ctcgaccaac gcctcggccg
     2521 catcaccctc gccggaccac gtgagcacga tccggtcgga cacgtcgtag tcgctggact
     2581 tgcgggcctc ctggaccagc cggacgaact cgcgggccag ccctgaccgc accagctcgg
     2641 gagtgaggtg cacatcgagc gcgatcgtct ccccgtgctc gttgacgacc gaccagccct
     2701 cgcgtggacg ctcggagagc acgacctcgt ctgcggtgac ctccacgccg ccgtcgaagt
     2761 cgacggtcgc ccgtcccgcc gtcgagaggc gttcggcaag ggccgacgcg tcggcaccgg
     2821 cgatcgcggc agccaccatc ggtgtctgct taccgaaccg cttcccgagc gtccggaagt
     2881 tggccttcgc agagtggtcg accagatcgc cggccgtggc gaacgactcc acacgcaaca
     2941 cgttcagctc cgcggcgacc tcctcccgca gctcgtcgga cagttcggcg tacgccgtcg
     3001 acgggatcag cgcccgttgc aacggctggc gtgtcttcac cttcgcctcg gcacgcgccg
     3061 accgcccgag ctcgacaagc cgtcgggcca gcgacatccc ctcgtcgagc cggtcgtcga
     3121 tgagctcctc gtcgtacgtc ggccaggtcg ccaggtgcac gctgtcggcg gtgccgtcgg
     3181 tacgccggaa caggtcctgc cagacccgtt cggtgacgaa cggggtgatc ggcgccagta
     3241 gccgggtgag cacgtgcaag gtctcgtgca gcgtggccaa cgcggccggg tcgccgtccc
     3301 agaagcgtct gcgagaacgc ctgacgtacc agttcgacag ctcgtcgacg aactccgcca
     3361 actgtgtccc gacccgctgc gtgtcgaagt cctccaacgc gagcgtgacg tcgcgggtca
     3421 agcggtggac ctgcgacaac aaccaccgat ccaggaccgg ccgccgatcc ggagccggcg
     3481 cggactggtc aggggtccag ccgttcgtcc gcgcatacag gacctggaac gcgaccgtgt
     3541 tccagtacgt caggagcacc ttccgcacga tctcctggag cgtggcgttg ccgacccgcc
     3601 gggccgccca cggtgagcct gacgcacaca tgaaccagcg caccgcgtcc gcgccgtgct
     3661 gctccatcaa cggcatcggc tcgaggatgt tgccgaggtg cttggacatc ttccgaccgt
     3721 cctcggcgag caacaggcca agacacacga cgttctcgta cgacgtctcg tcgaacagca
     3781 acgtgccgat cgccatcagc gagtagaacc agccacgcgt ctggtcgatt gcctcacaga
     3841 tgaactgcgc cgggaaggcc tcatggaacc gctcgtcggc gccgtcgacg tacgggtagc
     3901 cccattgggc gaacggcatc gagccggagt cgtaccacgc gtcgatcacc tcagtcacgc
     3961 gtcgtgcctc ggcaccgcac tccgggcagg gcaggacgac gtcgtcgacg tacggacgat
     4021 gcgggtccag atcattcagg tcccggcctg cgagctcacc cagttccgcc agcgaaccga
     4081 cacaggtgag gtggtcgtcg ccacaccgcc agatgggcag cggcgtaccc cagtaccggg
     4141 tgcgagacaa ggaccagtcg acgttgttgc gcagccagtc gccgtaccgg ccccacttga
     4201 tcgtctccgg ataccagttg gtcttctcgt tctcgcgcag caacgcgtcc ttgatcgccg
     4261 tggtgcggat gtaccaggcg ggcaaggcgt agtacatcag cggcgtgtgg cagcgccagc
     4321 agtgcgggta ggagtgctcg tgggcgacgt gccggaacat cagaccgcgg gactccagct
     4381 cccggaccag gtcggcgtcg gcgtgcttga agaactggcc gccgacgagc cggacctggt
     4441 cttcgaagtg tccgtcgcgg cggatcgggt tcacgaccgg catgccgtag ccctgtgtga
     4501 ctcgcatgtc ctccccaccg aaggcggggg actggtgtac cagacctgtg ccgtcctcgg
     4561 tcgtgacgta gtcggccagc acgacatggt gcgcacgtgg ttgcgcgccg gcgtctccac
     4621 gatcatggct ggggtcgtcg tcctcgctcg ggtcgtcggt cggaaagtcg acgaggtcga
     4681 gaggacgctg atagcgccag tactccatgt cgcggccggc acgacggtcg gtcacggtcc
     4741 atccgtcacc gagaacggac tggaagagcg gctcggcgac gatcagggtc tcgctgccgt
     4801 cggacgccac gacgtactcg acgtcggggt ggacagcgac cgcggtgttc gacacgagcg
     4861 tccacggtgt cgtcgtccac accagcagcg acgcctgtcc ggcgtacggt cctgaggtca
     4921 gcgggaaacg gacgtagatc gacgggtcga cgacggtctc gtaaccgccc ggttgaccga
     4981 gctcgtggtc cgagagtgcc gtctcgcagc gcgggcagta cggcgtcacg cggtgcgact
     5041 cgtagagcag gcccttctcg aagacctgct tcaacgacca ccacacgctc tcgatgtagg
     5101 tccggtccat cgtgcggtag gcctgggaca tgtcgaccca gtacgccatc cgctcggtca
     5161 tctcctcgaa gtcgtcggtg gatcgctcga cggactcccg gcacttcgcg ttgaactcgg
     5221 cgatgccgta ggcctcgatc tgctgcttgc cggagactcc gatctccttc tcgacgccca
     5281 cctcgacatg caggccctgg cagtcccacc ccgccttgcg gtcgactcgg aaaccgcgca
     5341 tggtcttgaa gcgcgggaag acatccttga agacacgcgc ttcgacgtgg tgcgttcccg
     5401 gcttgccgtt cgcggtcggt ggcccttcgt agaacgtcca ccgaggacct tcggcggtct
     5461 gttcgagcga ccggcggaac gtgtcgtgct cgcgccagaa acgcaggatc tcccgctcca
     5521 tcgcgggtag gtccacctgt ggcggcaccg gctggtagct gccgcccgag ctcgctggag
     5581 cggtgtcggt gctcatcgcg ggcctcacct tcgtcacgct tgcgtctgct gccgtcagcc
     5641 tgtcgcgaag ggacgacccc gccggtcggc gggtcccgcg gtaccaccct ccttggtcgg
     5701 tcctcgacga ggtccgaccc tctctggtcg cgccgcggtc ggttctaatg agcggatcga
     5761 gaccggggcc gatccaccgt tcttccgaca gctccgaggt gatggcccca tcccagccgt
     5821 acggcgcagg cacagtctat tcgcgtcacc gccgctgact catccgggtt tcggagtacg
     5881 ccgtctcggc gcaccacggg catcgacgct ctcggtgccg cgtggctgtt tgaagcacgg
     5941 ccgataaccg cctattctct ccgcaccgtg ttcgcgggga acacgggcga cccactgggc
     6001 ggccagggcc gaagtcaggc gacggagagg ccatcggatg gcggcgagaa gcacgtcggc
     6061 gatcaaggcg gtgaagcagg ccgtcggaaa ggcgacccgg cgaggcggcg ggtccgccgc
     6121 accgaaggcg gcatcggcga agacggcaac gcccgcgaag aaggccgcgt cggccaagaa
     6181 gaccgcgact gcgaagaagg ccgcgcccgc caagaagacg gcagccgcca agaaggccgc
     6241 gccggcgaag aaggccgcgc cggcgaagaa ggccccaccg gcgaagacgg ccgcgcccgc
     6301 gaagaagacg gcagccgcga agaagacggc agccgcgaag aaggccgcgc ccgccaagaa
     6361 actcagcgcc agcaaggcgg cgccggccac gagaacatcg cccggctcgc cgccggcaag
     6421 gaaggcagca gcattggcaa ccgacatcgg cgccgcaccc gcgcctgcga agaaggtcag
     6481 gggcggcaag ctcgcggttc gtgaggacga gaagccgtgg accgcagggg agatcaagga
     6541 ggtccgggcc aacctggagc atgacgccac caggctgaag gccgagatcg ccgccaccga
     6601 ggaggagatc ggacacctgc tccgggaggg tggggaaggc gccggcaacg accaggccga
     6661 cgtcggctcc aacacgttcg agcgggatca cgagatgacg atggcgaaga acgcccgtga
     6721 gtctctccac gtcatcgagg cggcgctgcg aaggatcgac gacggcacct acggcatctg
     6781 cgagtcgtgc gggcggccga tcggcaagat gcggctgcag gcgttcccgc gtgcgacact
     6841 gtgcatggaa tgcaaacaac gccaggaacg tcgctgaccg acgacacacc caccactcct
     6901 ccaccggctc ggcaccaccg catcctgctg tgggctgcgg tagccgcctt cgcgtacctg
     6961 ctggaccagg ccaccaagac ctgggcctcg cacacgctcg aggacggaca gagccgagcg
     7021 atcatcggcg acctgttgac cttccatctc acgcacaacc cgggggcggc gttcagcatg
     7081 gggacgggct acaccgtcgt cctcaccgtg atcgccctga cggtgatcgt cgtgtgcatc
     7141 cggatggccg gaaggctcgg gagcaccggc tgggcgatcg cgctcggcct gctgctcggc
     7201 ggggcactcg ggaacgtcac cgaccgcatc ttccgggcgc ctgcaccgtt ccgcggccac
     7261 gtcgtcgact tcatcgagat cccgcactgg ccggtcttca acttcgccga cacggccatc
     7321 agtatcgcgg cggtcctgtt cgtgctgcag agtgcccgag gagtgcacct cgacgggcgg
     7381 gtcgagcgcc gtcgtgatct cgacgacgag cccacggagc cgtgaccgtt cccgaatcct
     7441 cggtttcccc gggcgcgagg atcctgcatg tcccggcggg acttgccggc gagcgtgtcg
     7501 acgccgcgat cgcgcggatg ttcggtctct ctcggaccaa ggcggccgaa ctggtcgcgg
     7561 caggtgacgt gaccctcgac ggcgtacgac cggcgaagtc cgaccgggtc gaggagggcg
     7621 cgtttctcga ggtgctgatc cccgcacccg acccgggcgt tcgggtccgt cccgaggtcg
     7681 tggccgggct gcggatcatg tatgacgacc agtccttcgt cgtggtcgac aagccggttg
     7741 gtgtcgccgc ccacccgagc gtcgggtgga ccggccccac cgttctggga catctggccg
     7801 gggcgggttt ccggatcgcc acctccggcg tccccgaacg gcaggggatc gtgcaacggc
     7861 tcgacgtcgg cacgtccggt ctcatggtgg tggcaaagtc ggagtacgcc tactcaaggc
     7921 tcaagcgtgc gt
//
LOCUS       DATBFZ010000146         1551 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_4031939, whole genome shotgun sequence.
ACCESSION   DATBFZ010000146 DATBFZ010000000
VERSION     DATBFZ010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4031939"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="VH419_04410"
     CDS             <1..310
                     /locus_tag="VH419_04410"
                     /inference="COORDINATES: protein motif:HMM:NF020741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TilS substrate-binding domain-containing
                     protein"
                     /protein_id="HEY7042893.1"
                     /translation="RADADVLDEIAGDLRTRAVEATGDGVDVAILAASPSAVRRRVLR
                     RVALDAGSPATDLFALHVDELERLLVDWHGQGPIMLPGDVTATRHDGVIRFACTRGEA
                     "
     gene            374..925
                     /gene="hpt"
                     /locus_tag="VH419_04415"
     CDS             374..925
                     /gene="hpt"
                     /locus_tag="VH419_04415"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020387935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEY7042894.1"
                     /translation="MDVSHVEKDIARTLLTEDEIQQRLEELARQIEVDYAGTDLLLVG
                     VLKGACMVMADLARTLDRHVTMDWMAVSSYGAGTTSSGVVRILKDLDTDISGKHVLIA
                     EDIIDTGLTLSWLVANLQSRGPESVNICTLLRKPDALRMAVDVKYVGFDIPNEFVVGY
                     GLDHAEKYRNLRSVATLAPHVYS"
     gene            1178..>1551
                     /locus_tag="VH419_04420"
     CDS             1178..>1551
                     /locus_tag="VH419_04420"
                     /inference="COORDINATES: protein motif:HMM:NF018218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HEY7042895.1"
                     /translation="MKRWFRGPWLWVVLFAILILIVVDMVSKSDGSEQYDTSKMISTI
                     KAGDVSELTLVDGDQRIEATLDDGTKVTSNWAAAQGPALFKTIQQAQDDGKIQKTYNV
                     EVPKPSLLWSFLGTIFPILLIVL"
BASE COUNT          260 a          514 c          525 g          252 t
ORIGIN      
        1 gcgcgccgac gccgacgtgc tcgacgagat cgccggcgac ctccgcacgc gagctgtcga
       61 agcaacgggg gacggcgtgg acgtggcgat cctggcggcc tccccgtcag ccgtacgccg
      121 gcgggtcctg cgtcgcgtcg cgctggacgc cggctcaccc gcgaccgacc tgttcgcgct
      181 gcacgtggac gagctggagc ggcttctcgt cgactggcac gggcaggggc cgatcatgct
      241 gcccggggac gtgactgcca cccgtcacga cggcgtcatc cgctttgcct gtactcgtgg
      301 cgaggcctag acacggcgtc ggcgcggatg gcctgctgcg cgggaccgac gacgagggcc
      361 gtaccctgag cccgtggacg tcagtcacgt cgagaaggac atcgcccgga ccttgctcac
      421 cgaggacgag atccagcagc gactcgagga gctcgcccgc cagatcgagg tcgactacgc
      481 cggcaccgac ctgttgctcg tcggggtgct caagggcgcc tgcatggtga tggccgacct
      541 ggcgcgcacc ctcgaccggc acgtcacgat ggactggatg gcggtgtcgt cgtacggcgc
      601 gggcacgacc agctccggcg tcgtgcggat cctcaaggac ctcgacaccg acatctccgg
      661 caagcacgtg ctgatcgcgg aggacatcat cgacaccggg ctcacgttgt cgtggctggt
      721 cgccaacctc cagtcgcggg ggccggagtc cgtcaacatc tgcacgctgc tgcgcaagcc
      781 ggacgccctg cgcatggcgg tcgacgtcaa gtacgtcgga ttcgacatcc cgaacgagtt
      841 cgtggtcggc tacggactgg accacgccga gaagtaccgc aacctgcgct cggtggcgac
      901 gctcgccccg cacgtctact cctgagtgtc ggctccgtcg gctccgtcgg cagggtcggg
      961 accggatctc cgacagctcc ggtcgacgag acgggcaggg gccctgacca ggccggatca
     1021 gcgaagttgc cccgattttc ggtggtcctg cccaccgatt tggtccgtag gtgacaatgg
     1081 aagaccaagg atggctgcga cggtgtctgc cgcgccttga cgtagcactg tcgaacgacc
     1141 ggtaggaggc acggggccga cggctccggt aagtagcgtg aagcgttggt tccgtggacc
     1201 ctggttgtgg gtcgtcctct tcgcgatcct catcttgatc gtggtcgaca tggtctccaa
     1261 gagcgacggc tcggagcagt acgacacctc caagatgatc tcgaccatca aggccggcga
     1321 cgtcagcgag ctcaccctgg tcgacggcga ccagcggatc gaggcgacgc tcgacgacgg
     1381 cacgaaggtc acctcgaact gggcagccgc gcagggtcct gcgttgttca agaccatcca
     1441 gcaggcgcag gacgacggca agatccagaa gacctacaac gtcgaggttc cgaagccgag
     1501 cctgttgtgg agcttcctcg gcacgatctt cccgatcctg ctgatcgtgc t
//
LOCUS       DATBFZ010000147         9473 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_1733172, whole genome shotgun sequence.
ACCESSION   DATBFZ010000147 DATBFZ010000000
VERSION     DATBFZ010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 9473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9473
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1733172"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1766
                     /locus_tag="VH419_04425"
     CDS             <1..1766
                     /locus_tag="VH419_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042896.1"
                     /translation="VSSDAGYGERAAVNDPRPREGYALAGRSGSVVEVATDEAHTPPA
                     PRRSRLRTVCYAVTTALAGLVVWLALVAPNEIEDVTPRTFLRIPLELLVVVALALVLR
                     PWARRTVAAVFGVLLGLVVIVKVLDMGFKAVLDRSFDPLSDWYYLGPGVGVLGDSVGH
                     TAATAITVVAAILVLALLVVLPLCAVRLSSFAADHRRTSIQVVTVLGLVWVLCAVSGP
                     VFAPAARLATASSAGLAYDEVRQLQADFADRKVFAKEIAADRFADVPGDQLLTALRGK
                     DVLLVFVESYGRIAVQDSSFSPGVDAVLDSGTRQLRAAGYSSRSAFLTSPTFGAASWL
                     AHSSLQSGLWVDSQQRYNQVVTSDRLTLATAFSRAGWRTVSDVPSNTKDWPDGSSFYH
                     FDKQYDARNVGYQGPSFSYASMPDQYTLAALRRLELAKTDRPPVMAEIDLVSSHHPWT
                     PLPHLVDWDQVGDGSVFDGMPEQGESPDEAFRDPDEVKALYGQSIEYSLSSVISFLQT
                     YPDPDLVVVMLGDHQPHSYVTGDNPGHDVPISVIAQDRSVMRRISGWGWQGGLHPAPD
                     APVWPMDTFRDRFLTAYGPNP"
     gene            2404..3054
                     /locus_tag="VH419_04430"
     CDS             2404..3054
                     /locus_tag="VH419_04430"
                     /inference="COORDINATES: protein motif:HMM:NF023144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HEY7042897.1"
                     /translation="MTLGFAGSLDRSAVLQAADLVAELVARSEVAQAWTSESSCVGMS
                     VGGLTRHLVSQPVNVVKLLRADQSDAETIPLLEHYARAAWVREDLEGDTNRSIRESAD
                     REASEGRDTALALLAQARSEIESTLADAPSTTYIPWQGWSLATDDFLVTRLMEMVVHA
                     DDLAASVAVPTPEFGTDVLDPVFRLLTAIAVRRHGQDAVVRTFARPQRAPRDVAAF"
     gene            3185..4798
                     /locus_tag="VH419_04435"
     CDS             3185..4798
                     /locus_tag="VH419_04435"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY7042898.1"
                     /translation="MTQPSDPTEERVDPVEAAGAVEAVHGGQAAHEEPDPQRWWALAV
                     LAVAQLMIVLDASIVNIALPSAQADLDISNANRQWIVTAYTLAFGGLLLLGGRIADYN
                     GRKRTFIIGLLGFAVASAIGGFAPNQEFLFAARALQGGFAALMAPAALSIVTVTFTEP
                     KERAKAFGVFGALAGGGAAIGLIVGGVLTEYASWRWCLGVNVPVALLTAAAAIPIVHE
                     SKARGDTRYDIPGVLLATVGLVSLVYGFTEAAKAKNPADPTDTRVQGWGASSTTWFIV
                     IAVILLVAFVVWERRAPNPMLPLRVVLHRNRGGSYLLFLLVGAGLFAMFLFLTYYFQV
                     NLGYSPLKAGFAFLPFSLGIILTAGAVAQLLPRLGPKPLIVVGLAMAVIGMLMLTQIT
                     EDTSYWSHVFIPEVLMSVGLAGVFIPAASTALIGVGHHDAGVASAVLNTAQMIGGSLG
                     TALLNTVFAGAVTAYMTDNLTNPADIPQLLPSAFLHGYHVSFLWGAALFFAAWLVGVF
                     IINASKEDVPKDPAMVMAADDDEEDPVTP"
     gene            complement(4825..5517)
                     /locus_tag="VH419_04440"
     CDS             complement(4825..5517)
                     /locus_tag="VH419_04440"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY7042899.1"
                     /translation="MAEVSGVVYLVRHGQASLFGRDYDRLSDLGLKQSRTVGETLAAR
                     GVVPALVVRGSLERHRQTAETAIAAAGWDAPVHVDPLWAEIDHVDIIGALRPTYTSHA
                     IMVDDLLTRPEPERAFRSIFDDALQVWLTGGASYIETHLEFRDRVDAALGSVVGRLGR
                     GGTAVVFSSGGPIAGVVASTLGLDTAGWLRMSRLVVNASVTKLVVGESGITLLSFNEH
                     AHVEARDQVTYH"
     gene            complement(5510..6634)
                     /locus_tag="VH419_04445"
     CDS             complement(5510..6634)
                     /locus_tag="VH419_04445"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEY7042900.1"
                     /translation="MAESVVAGRPGRSPDDLSRAVPVRAEDTFDVAAVAQWLTEHGVP
                     GAESGAVPDVRQFPGGASNLTYELRYRRHRLILRRPPHGSRVRSAHDMRREFTVQSAL
                     VGAFPYVPRMLALCTDLDVIGSDFYVMEKVDGLILRTDPPPGFGLDKAATRRLCLTFV
                     DRLVELHSVDVQAVGLGGLSRGDGYVARQVEGWTERFRRARTPDVPDYERTIGWLAAS
                     RPPDVAARLIHNDFRFDNVVLASDDHERIVAILDWEMATVGDPLMDVGGALAYWAQAD
                     DDPTMLRLRRQPTHLPGMLTRSEVWDRYADLTGFDTSGRRFYEVFGLFRLAVIAQQIY
                     YRFREGQTHNPAYGRFHELVTYLDHRIEQDLLADRSEGRG"
     gene            complement(6627..7460)
                     /locus_tag="VH419_04450"
     CDS             complement(6627..7460)
                     /locus_tag="VH419_04450"
                     /inference="COORDINATES: protein motif:HMM:NF004196.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY7042901.1"
                     /translation="MTERVLVTGSASGLGQALARRYADRGAHVLVTDVATEVDPTLLP
                     AAREQGEVSYLQLDVRDADGWDRARTWVVEHWGGLDVLVNNAGVAAAGRFDVVPIEDW
                     EWIVDTNLLGVARGCRAFVPLFKEQRSGRIVNVASMAGLVHPPCMASYNAVKAGVVAL
                     SETLLHELAPFGIGVCVTCPSFFRSNLADSIVRGSDPAVERSGAGLVSGSRVSADLIA
                     VRTIAAVDKGKFLVLPSREGRVAWYGKRLASPVYHWAMKRVGARVMRKAAGREVAAGR
                     G"
     gene            complement(7457..8683)
                     /locus_tag="VH419_04455"
     CDS             complement(7457..8683)
                     /locus_tag="VH419_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020386760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY7042902.1"
                     /translation="MDFSHSPRSREYQERVAAFMRDEIEPVEADYHHAVAASDDPWTP
                     LPLLAELQGKARATGLWNLFMSDEKYGAGLSNVDYAPCAELMGRSALAPHVFNCNAPD
                     TGNMEVLAAYGTEEQRRRWLEPLLDGEIRSAFCMTEPEVASSDATNMSASAVLDGDEV
                     VINGRKWWSTGIGHPRCEILLFMGLTDAEAPRHARHSIVLVPRSAPGVRVERMLSVMG
                     MDDAPFGHGEVSFHGVRVPVANVIGTPGQAFEIAQSRLGPGRIHHCMRLVGLAERALE
                     LAVDRGLRRTAFGERLIDLGGNRERIADARIAIEQLRLLVLRTAWLLDTHGVRAARSE
                     ISQIKVAAPRVAQDVVDVAIQLHGAGGLSADQPLAAAWANARSLRLADGPDEVHRSLV
                     ARLEVARHEQAYGEAR"
     gene            complement(8940..>9473)
                     /locus_tag="VH419_04460"
     CDS             complement(8940..>9473)
                     /locus_tag="VH419_04460"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEY7042903.1"
                     /translation="GLMAGTVDVLRRQWESSSHRLRERLVGLTDDEFRWEPVPGCWNV
                     RRVGDSWTMDYPDVAPDPPPVTTIAWRLLHITHGTWMYADHAFGSGTMSFLDLEIHGT
                     AAAAVEDLVASQQRLADVLDRLDDAGLERDVATHLGDEPWTAAAVITTLIDEQIHHGA
                     EIGLLRDLYLRLGPADD"
BASE COUNT         1496 a         3370 c         3145 g         1462 t
ORIGIN      
        1 ccgtgtcttc cgacgcgggg tacggtgaac gggccgcggt gaatgatccc cgaccgaggg
       61 aggggtacgc gttggcggga cggtcaggct cggtcgtcga ggtggcgacc gatgaggcgc
      121 acacgcctcc agcgccgagg agatcgcggc tccggaccgt ctgctacgcg gtgacgaccg
      181 ccctcgccgg cctcgttgtc tggctggcgc tggtcgcccc gaacgagatc gaggacgtga
      241 cgccgaggac gttcctgcgg ataccgctcg aactgctcgt cgtcgtcgcg ctggccctcg
      301 tgctccgccc gtgggcacgc cggacagtgg ctgcggtgtt cggtgtgctg ctcggcctcg
      361 tggtcatcgt gaaagtcctc gacatgggct tcaaggccgt gctcgaccgg tcgttcgacc
      421 cgctcagcga ctggtactac ctcggacccg gtgtcggcgt gctgggcgac tcggtcggtc
      481 ataccgccgc gacggccatc acggtcgtgg cagcgatcct cgtgctcgcc ctgctcgtcg
      541 tgctcccgct ctgcgctgtc cggctgagca gcttcgccgc agaccaccgg cgtacgtcga
      601 tccaggtggt caccgtgctt ggcctcgtat gggtgctgtg cgcagtctct ggcccggtgt
      661 tcgccccggc cgcccggctg gccacggcga gctcagccgg tctcgcgtat gacgaggtgc
      721 gacagctgca agccgacttc gcggaccgga aggtattcgc gaaggagatc gcggccgacc
      781 ggttcgccga cgtccccggt gaccagctgc ttacggcgct gcgaggcaag gacgtgttgc
      841 tcgtgttcgt cgagagctac gggcggatcg cagtccagga ctcgtcgttc tcgccgggcg
      901 tcgatgctgt gctggacagc gggacccgac agctccgggc cgccggctac tcgtctcgca
      961 gcgcgttcct gacctccccg acgttcggcg ccgcgagctg gttggcccac tcgtcgctgc
     1021 agtcgggcct gtgggtcgac agccagcagc ggtacaacca ggtcgtcacg agcgaccggc
     1081 tcaccctggc cacggcgttc tcgcgggcgg gctggcggac ggtgtccgac gtaccctcca
     1141 acaccaagga ctggccggac gggagctcct tctaccactt cgacaagcag tacgacgccc
     1201 ggaacgtcgg ctaccagggg ccgagcttca gctacgcgtc gatgcccgac cagtacacgt
     1261 tggcggcgtt gcggcggctg gagctggcga agaccgatcg gccgccggtg atggcggaga
     1321 tcgacctcgt gtcgagccat catccctgga cgccgttgcc ccacctcgtc gactgggacc
     1381 aggtcgggga cggctcggtc ttcgacggga tgcctgaaca gggggagtca cctgacgagg
     1441 ccttccgtga cccggacgag gtgaaggcgc tgtacgggca gtcgatcgag tactcgctca
     1501 gcagcgtgat ctcgttcctg cagacctatc cggatcctga cctcgtcgtg gtcatgctcg
     1561 gcgaccacca accgcactcg tacgtcaccg gagacaaccc gggccacgac gtaccgatca
     1621 gcgtcatcgc gcaggaccgt tcggtgatgc ggcggatctc cggctggggc tggcaaggcg
     1681 gcctgcaccc ggctcccgac gcgccggtgt ggccgatgga cacgttccgg gatcgcttcc
     1741 tcaccgccta cggccccaat ccgtagacac catccgcgag agcagatccg gtgagccgcg
     1801 cctgctaccg gtgcattcct cacccgtcaa gggtcgctat cgcgatcgct gacgcgacgg
     1861 accgcgtccg cccttgaccc gtgagcctcg atgcaccggc cgggcaggct ccgtcgggca
     1921 gcatttcgtc ctgcccatcc aacggcgcgg cccacccgcc accagaaagc cacctaccgc
     1981 accaatcaga cacgcgtcgg tggtggtttg cctgggtagc catccaaacg gacggttaac
     2041 caggcagatc tgccttggga gggatggagc cgcctcggta gccatgcaaa ccgcccgccg
     2101 gcgacccacg gatgtccgaa acccgccagt cacgaacccg gccacaacgg acgggaaagc
     2161 cggccagcca gctcagcagg caacaagctt gtccgttctt cattgcgacc cacctcagcc
     2221 cagcacgaca gtgcgccggc cgaccacgcc cgcccacggc gaccatctcc gccctccgaa
     2281 gccggagaag gactcaggtt tgcctcggta gccgtgcatc cggatggctg cccaggcaaa
     2341 caaccacggc cagatgcccg cggtgggact cgttgtccgt gcggctgact agactccgcg
     2401 agcgtgacgt tgggattcgc cggaagtctt gatcgcagcg ccgtcctgca ggcagccgac
     2461 ctggtcgcgg agctggtcgc gcggtccgag gtcgcccagg cctggacgag cgagtccagc
     2521 tgcgtcggga tgtcggtagg cggcctcacc cgacacctgg tcagccagcc cgtcaacgtc
     2581 gtcaagctgt tgcgcgccga ccagtccgac gcggagacga tcccgctgct cgagcactac
     2641 gcgcgagcag cctgggttcg cgaggacctg gagggtgaca cgaaccgctc catccgcgag
     2701 agcgcagatc gggaggcatc ggagggccgc gacaccgcac tggcgttgct cgcgcaggcg
     2761 cggtccgaga tcgagtccac gctcgccgac gcgccctcga cgacgtacat tccgtggcag
     2821 ggctggtcgc tggcgaccga cgacttcctc gtcacccgcc tcatggagat ggtggtccac
     2881 gccgacgacc tggcggcgag tgtcgcagtg cctacgccgg agttcgggac cgacgtgctg
     2941 gatccggtgt tcaggctgct caccgcaatc gccgtacgcc gacacggcca ggacgccgtg
     3001 gtccggacat tcgcgcggcc gcagcgcgcc ccgcgggacg tcgcggcctt ctgagcaagc
     3061 ggtcgggtcg cgccgccatg tgggaaattc actgaggcct gtcacaggca gatggcagac
     3121 tctccccttg tgcacgccgc tcgcctcgag gggcgaccgc ggcccgactg acggggaggt
     3181 ccgaatgacg cagccgagcg accccactga ggagcgggtc gaccccgtcg aggccgccgg
     3241 cgccgtcgaa gctgtgcacg gtggtcaggc ggcccacgag gagcccgatc cgcagcgctg
     3301 gtgggcactc gcggtgctcg ccgtcgcgca gctgatgatc gtcctcgacg cctcgatcgt
     3361 gaacatcgcc ctgccctcgg cccaggccga tctggacatt tccaacgcga accggcagtg
     3421 gatcgtcacg gcgtacacac tggcgttcgg tggcctactg ctgctgggtg gtcggatcgc
     3481 ggactacaac ggccgcaaac gcaccttcat catcggactc ctgggcttcg cggtcgcttc
     3541 cgcgatcggt gggttcgcac ccaaccagga gttcctcttc gccgcccggg cgctccaggg
     3601 cggtttcgcg gcactgatgg cgccggccgc gctgtccatc gtcacagtga cgttcaccga
     3661 gccgaaggag cgggcgaagg cgttcggtgt gttcggggcg ctggccggtg gcggtgcagc
     3721 gatcgggctg atcgtcggag gcgtgctgac ggagtacgcc tcgtggcggt ggtgcctcgg
     3781 tgtcaacgtg ccggtcgccc tgctcacggc agcagcggcg attccgatcg tgcacgagag
     3841 caaggcgcgc ggcgacacgc gatacgacat ccccggcgtg ctgctcgcga cggtcggtct
     3901 ggtctcgctg gtctacggct tcaccgaagc cgccaaggcg aagaaccccg ctgacccgac
     3961 cgacacccgc gtgcagggct ggggggcgtc gtcgacgacc tggttcatcg tcatcgccgt
     4021 catcctcctc gttgccttcg tcgtgtggga gaggcgtgca ccgaacccga tgctgccgct
     4081 gcgcgtcgtg ctgcatcgca accgtggcgg ctcgtacctg ctcttcctgc tggtcggcgc
     4141 aggcctgttc gcgatgttct tgttcctcac gtactacttc caggtcaacc tcggctacag
     4201 cccgttgaag gccggcttcg ccttcctgcc gttcagcctg gggatcatcc tcaccgccgg
     4261 cgcggtggcc cagctcctac cacgcctcgg gccgaagccg ctgatcgtgg tcggtctcgc
     4321 catggccgtc atcggcatgc tgatgctcac ccagatcacc gaggacacgt cgtactggtc
     4381 gcacgtcttc atccctgagg ttctgatgag cgtcggactc gccggggtct tcatcccggc
     4441 cgcgagcacg gcgctgatcg gcgtgggcca ccacgacgcc ggcgtcgcga gcgccgtcct
     4501 caacaccgcg cagatgatcg gtggatcgct cggcaccgcg ctgctcaaca cggtgttcgc
     4561 cggggcggtc acggcgtaca tgaccgacaa cctgaccaac ccggccgaca taccgcagct
     4621 ccttccatcg gcgttcctcc acggctacca cgtctcgttc ctgtggggcg ccgccctgtt
     4681 cttcgccgcc tggctggtgg gcgtgttcat catcaacgcc agcaaggaag acgtccccaa
     4741 ggacccggcg atggtgatgg cggccgacga cgacgaggag gatccggtca ccccgtgaca
     4801 gtggatccgc agcaaggctg acgatcagtg gtaggtgacc tggtcacgcg cttcgacgtg
     4861 cgcatgctcg ttgaacgaca gcagggtgat ccctgactcg ccgacgacga gcttcgtcac
     4921 gctggcattg acgaccagcc tgctcatgcg cagccaacct gcggtgtcca gccccagcgt
     4981 cgatgcgacc acacccgcga tcggtccacc cgagctgaag acgacggccg tgccgccgcg
     5041 acccagccgc ccgacgaccg agcccaaggc ggcgtcgacc cggtcacgga actccaggtg
     5101 ggtctcgatg tacgacgccc cgcctgtcag ccagacctgc aacgcgtcgt cgaagatgct
     5161 gcgaaaggct cgctccggct cgggcctggt cagcaggtcg tcgaccatga tcgcgtgtga
     5221 ggtgtacgtc ggccgaagtg ctccgatgat gtccacgtgg tcgatctccg cccacagcgg
     5281 gtcgacgtgc accggcgcgt cccatcccgc ggcggcgatg gcggtctcgg cggtctgccg
     5341 atgccgctcg aggctgccgc gcaccacgag tgcgggaacc acgccgcgag ccgccagcgt
     5401 ctcaccgacc gtccggctct gcttcagtcc gaggtcggag agcctgtcgt agtcacggcc
     5461 gaacaacgac gcctgcccgt gccgcaccag gtagacgaca ccgctcacct cagccacgcc
     5521 cttcactgcg gtccgcgagg agatcctgct cgatccggtg gtcgagatac gtcaccaact
     5581 cgtgaaacct cccgtacgcc gggttgtgcg tctggccctc gcggaaccgg tagtagatct
     5641 gttgggcgat caccgccagc cggaacaacc cgaacacctc gtagaagcgg cggcccgacg
     5701 tgtcgaaccc cgtcagatcc gcataccggt cccacacctc cgaccgggtg agcatgcccg
     5761 gcaggtgggt cggctgccgt cgcagccgca gcatggtcgg atcgtcgtcg gcctgcgccc
     5821 agtacgcgag cgcgccaccg acgtccatca acgggtcacc gaccgtcgcc atctcccagt
     5881 cgaggatggc aacgatccgc tcgtggtcgt cagatgccag caccacgttg tcgaagcgga
     5941 agtcgttgtg gatcagccgc gccgccacgt ccggtgggcg gctggccgcc agccagccga
     6001 tcgtccgctc gtagtccggg acgtcagggg tacgtgcccg gcggaagcgc tcggtccagc
     6061 cctcgacctg acgcgcgaca tagccgtcgc cgcgggagag tccgcccaac cccaccgcct
     6121 gtacgtcgac cgagtgcagc tcgaccaacc gatcgacgaa cgtcaggcac agcctgcgtg
     6181 tcgccgcctt gtcgaggccg aatcccggcg gcgggtcggt gcggaggatg agaccgtcga
     6241 ccttctccat cacgtagaag tcggacccga tcacgtcgag atccgtgcac agcgcgagca
     6301 tgcgtgggac gtaggggaac gcgccgacga gcgccgactg gacggtgaac tccctccgca
     6361 tgtcatgcgc gctcctgacc cggctcccgt gcggcggccg gcgcaggatc agccggtgac
     6421 gccgatagcg cagctcatac gtcaggttgg acgcgccgcc agggaactgc cggacatcgg
     6481 gaacggcccc gctctccgcg ccaggcaccc cgtgctcggt cagccattgc gcgaccgccg
     6541 ccacgtcgaa ggtgtcctcg gcacggacgg gaacggcgcg ggagaggtca tcaggcgacc
     6601 ggcccggccg ccctgcgacg accgactcag ccacggcccg ccgcgacttc gcgccccgcg
     6661 gccttccgca tcacgcgcgc gccgacgcgc ttcatcgccc agtggtagac gggactcgcg
     6721 agccgcttgc cgtaccacgc gacccggcct tctcgcgacg gcagcaccag gaacttcccc
     6781 ttgtccacgg ccgcgatcgt ccggacggcg atcaggtcgg ccgacacgcg tgagcccgag
     6841 acgaggccgg ctccggaccg ctcgacggcc gggtctgatc cccgcacgat gctgtcagcg
     6901 aggttcgacc tgaagaacga cggacaggtc acgcagacgc cgatgccgaa tggcgcaagc
     6961 tcgtgcagga gggtctccga caaggcgacg acaccggcct tcacagcgtt gtacgacgcc
     7021 atgcagggcg ggtggacgag cccggccatc gaggcgacgt tcacgatgcg gccggaccgc
     7081 tgctccttga acaacggcac gaacgccctg cagcccctgg ccacgccgag caggttcgtg
     7141 tcgacgatcc actcccagtc ctcgatcggc accacgtcga agcggcctgc ggcggcgaca
     7201 ccggcgttgt tcaccaggac gtcgagacca ccccagtgct cgaccaccca ggtccgcgcc
     7261 ctgtcccaac cgtccgcatc tcggacgtcg agctgaagat aggagacctc gccctgctct
     7321 cgcgcagccg gcagcagcgt cgggtcgact tccgtcgcca catcggtgac gagcacgtgc
     7381 gctcctcgat cggcgtaccg gcgggcgagc gcctgaccca gtccggaagc cgaacccgtc
     7441 acgagcaccc gctctgtcac cgcgcctccc cgtacgcttg ctcgtgccgc gcgacctcga
     7501 gccgcgcgac cagcgaacga tgcacctcgt cgggcccgtc ggccaaccgg agcgagcgtg
     7561 cattggccca ggcggcggcg agcggctggt cagcggagag accacctgcc ccgtgcaact
     7621 ggatggcgac gtcgacgacg tcttgcgcca cgcgcggggc ggccactttg atctggctga
     7681 tctcgctgcg ggccgcgcgc accccgtggg tgtccagcag ccaggcggtc cgcagcacga
     7741 gcaggcgcag ctgctcgatc gcgatccgcg cgtcagcgat gcgttcccgg ttgccgccga
     7801 ggtcgatcag ccgctcgccg aacgccgtac gccgcaggcc gcggtcgaca gcgagctcga
     7861 gggcccgctc ggcaaggccg accagacgca tgcagtggtg gatccggcca ggcccgagcc
     7921 ggctctgcgc aatctcgaac gcctggcccg gcgtcccgat gacgttggcc acggggacgc
     7981 ggacaccgtg gaacgacacc tccccgtggc cgaacggcgc atcatccatc cccatcaccg
     8041 acagcatgcg ctcgacccgc acgcccggtg ccgaacgcgg caccagcacg atcgaatggc
     8101 gggcatgacg gggggcctcg gcgtccgtca aacccatgaa gagcaggatc tcgcagcgcg
     8161 ggtgcccgat gccggtgctc caccatttgc ggccgttgat cacgacctcg tcgccgtcga
     8221 gcaccgcgct cgcggacatg ttcgtggcgt cggacgacgc gacctcaggc tcggtcatgc
     8281 agaacgctga acggatctca ccgtcgagca gcggctcgag ccagcgccgc ctctgctcct
     8341 ccgttccgta tgccgcgagg acctccatgt tgccggtgtc aggcgcgttg cagttgaaga
     8401 catgcggcgc caacgccgac cggcccatca gctccgcgca tggcgcatag tcgacgttgg
     8461 acaggccggc gccgtacttc tcgtcggaca tgaacaggtt ccagaggccg gtggcacgag
     8521 ccttgccctg cagctcagca agcaacggca acggagtcca cgggtcgtca ctggcagcga
     8581 cggcgtgatg gtagtcagcc tcgaccggct cgatctcgtc gcgcatgaac gccgcgaccc
     8641 gctcctggta ttcgcgactg cgcggcgagt ggctgaagtc cacccgtcga ccctagcgac
     8701 agggagcctc agcgggccga gacggtggcg acgacccagg aacgcacctt cggccgggtc
     8761 cagtcgccgg ccgtgatcca cgccagagcg cacccgtcgc gtccaggcac cacgcgttga
     8821 cgccgatgag cagcgcatcc ggttgccgcg cggccacctc aggccacccc gcacacgctc
     8881 aaccgttcgg tcgagggacg ctcggcggct cgctgagccc agccgccgtt cccgggagct
     8941 cagtcgtcag ccggaccgag ccgcaggtag aggtcgcgga gcagcccgat ctctgctccg
     9001 tggtggatct gctcgtcgat cagcgtcgtg atgacggcgg cggcagtcca gggctcatcg
     9061 ccgaggtgcg tcgcgacatc gcgttcgagt ccggcgtcgt cgaggcgatc cagcacgtcg
     9121 gcgagacgct gctgactggc gaccaggtcc tcgacggcgg cagctgcggt gccgtggatt
     9181 tccaggtcga ggaacgacat ggtgccggag ccgaaggcgt ggtcggcata catccaggtg
     9241 ccgtgcgtga tgtgcagcag tcgccaggcg atcgtcgtca ccggaggagg gtcgggcgcc
     9301 acgtcggggt agtccatggt ccagctgtcg ccaacgcggc gaacattcca gcagccgggc
     9361 accggctccc accggaactc gtcgtcggtc agaccgacga gacgttcgcg caaccggtgc
     9421 gacgacgact cccactgccg gcgcaggacg tcgaccgtgc cagccatgag acc
//
LOCUS       DATBFZ010000148         5102 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_4032464, whole genome shotgun sequence.
ACCESSION   DATBFZ010000148 DATBFZ010000000
VERSION     DATBFZ010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 5102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5102
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4032464"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="VH419_04465"
     CDS             complement(<1..353)
                     /locus_tag="VH419_04465"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012347.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEY7042904.1"
                     /translation="MSAYLPVAAESDIEIGEHHTFEFLGMTFNADTIWSTAIAAAIVL
                     ILGLFVARGASSRSPGKMQLLWELLVDWVTTQVEANLGKVNPFVVPLAIALFVFILVC
                     NWLELIPTHDWLKSPT"
     gene            complement(356..757)
                     /locus_tag="VH419_04470"
     CDS             complement(356..757)
                     /locus_tag="VH419_04470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042905.1"
                     /translation="MTGPAESSAQALRNQKRSALLALGMVVAAMWISIPMGEWRVGVF
                     VSVGIVLSFVNHALAELSLLRSVESGDEITKRQYAVTSLVRLMAVSVVAITLAVVFWP
                     DGAAVLVGLALFHLITLVFVGIPLLKEIKKA"
     gene            complement(803..1021)
                     /locus_tag="VH419_04475"
     CDS             complement(803..1021)
                     /locus_tag="VH419_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042906.1"
                     /translation="MTGDEEPPTLGAWDLVGLGGLNLACLVVGLGIGWFVDDASGQAP
                     VFTLVGLAVGITVGIVVSWIRIRRFLQT"
     gene            complement(1527..2666)
                     /locus_tag="VH419_04480"
     CDS             complement(1527..2666)
                     /locus_tag="VH419_04480"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HEY7042907.1"
                     /translation="MREYVLIFVVALSVTYLLAVIARELAMKFGAYARVRDRDVHEIP
                     IPYFGGVAMMAGLAAAYLVATQLPFLGKRQLIVDDARAVLLGGFVICLVGVIDDIFEL
                     DAITKLAGEVVAAGVVVLQGIQLLWLPLPGERSMSAFSLGSSQSALLTVIIIVTTVNA
                     VNFVDGLDGLAAGVVGIGAVAFFTYAVLLALVNDQTRASTVAMLTAALAGVCFGFLPH
                     NFFPARMFMGDSGALLIGFMLACSAISLTGQYPTSRIDQGLFGANASLLPALLPLILP
                     FAVLVVPFADLVLAVVRRTRAGRSPFSPDKKHLHHRLLEIGHTQRRAVLVMYLWAGLV
                     AFGVVLISLFSGWWSVLGLSSMAVVALLLTFGVPGHSGIIKTEDA"
     gene            complement(2666..3442)
                     /locus_tag="VH419_04485"
     CDS             complement(2666..3442)
                     /locus_tag="VH419_04485"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEY7042908.1"
                     /translation="MSTTEPDSSPAPGVYDLADPAQRSAGFAEAKEAFGRSGLVVFPT
                     DTVYGLAADAFDPTGVRRLLRAKGRGRNMPTPVLIGGNDTLRALATNLTPDVRALASA
                     FWPGGLTLICRQQPSLRWDLGDSRSTVALRMPDQPDALDLLADNGPLAVSSANITGQP
                     PATTARQAQDMLGESVDVYLDGGPTPGSLPSTIVDCTGDALRVVRVGVVSVHALREVV
                     SDLEAPADALADDSDDSDGSDGSDGSDDDSADGGQDTGRA"
     gene            complement(3439..4350)
                     /gene="prmC"
                     /locus_tag="VH419_04490"
     CDS             complement(3439..4350)
                     /gene="prmC"
                     /locus_tag="VH419_04490"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012922630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEY7042909.1"
                     /translation="MTYDLRTLLADATRTLADGGVPSPRHDAEMLAAHVMRTQRPALA
                     GISTVTQVQREQFHHLVRRRAGREPLQHLTGRVGFRYLDLEVGPGVFVPRPETEVMTG
                     VAVDELRQLVVAGVAPRAVDLCTGCGAVAIALGTEVPQAQVVGIELSPEAAAYAERNA
                     RGTGVDIRVGDLADAARSLADLAGSVHVVTANPPYIPLEAWESVDVEVRDFDPALALW
                     SGDDGLDAIRLVAHQAAELLVDGGLVTCEHADVQGESAPRVFVDSGLWCDVRDHPDLT
                     GRPRFVTARRVPRAARPDGTSPGTIGA"
     gene            complement(4347..>5102)
                     /locus_tag="VH419_04495"
     CDS             complement(4347..>5102)
                     /locus_tag="VH419_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020575209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEY7042910.1"
                     /translation="KSGEGGEESALFAGDLLRMYMRYAERRGWKTEQIDATESDLGGY
                     KSVTVAVKAKGTPEPGEAPYALLKFEGGVHRVQRVPVTESQGRIHTSAAGVLVMPEAE
                     QLDVTVDENDLRVDVFRSSGPGGQSVNTTDSAVRITHLPTGIVVSCQNEKSQLQNREQ
                     AMRVLRSRLLQVAQEQRDAENADARRSQVRTVDRSERIRTYNFPENRITDHRVGYKAY
                     NLDQVLDGDLQAVIDADVQADLADKLAAVESAP"
BASE COUNT          873 a         1724 c         1755 g          750 t
ORIGIN      
        1 gtcggtgact tgagccagtc gtgggtcggg atcagctcga gccagttgca caccaggatg
       61 aagacgaaga gtgcgatcgc cagtggcacg acgaacggat tgaccttgcc gaggttcgcc
      121 tcgacctggg tcgtgaccca gtcgaccagg agctcccaca gcagttgcat cttgcccggc
      181 gaacggctgc tggcgccccg tgcgacgaac aggccgagga tgagcacgat ggctgcggcg
      241 atcgcggtgg accagatcgt gtccgcgttg aacgtcatac cgaggaactc gaaggtgtgg
      301 tgctccccga tctcgatgtc gctctcggcc gcgaccggca gataggcgct catgctcatg
      361 ccttcttgat ctccttcagc agcgggatgc cgacgaacac caacgtgatc aggtggaaca
      421 gcgccagccc gaccagtacg gcggcaccgt ccggccagaa gacgaccgcc aacgtgatgg
      481 cgacgaccga gacggccatc aggcgcacca gcgacgtcac ggcgtactgg cgcttggtga
      541 tctcgtcacc cgactcgacg gagcgcagca gggacagctc agccagcgcg tggttgacga
      601 agctgagcac gatgcccacc gagacgaaga cgccgacgcg ccattcgccc atcgggatcg
      661 agatccacat cgccgcaacg accatgccga gcgcgagcaa cgccgaacgc ttctggttgc
      721 gcagcgcctg ggctgacgac tcagcggggc cggtcaccgg cacactcctt cgggaatctc
      781 gcggacgctc tggtcggtcg ggctaggtct ggaggaaccg ccgaatgcgg atccacgaca
      841 cgacgatgcc gacggtgatg ccgaccgcga gtccgaccaa ggtgaacacg ggtgcctgcc
      901 cactcgcgtc gtcgacgaac cagccgatac ccagtccgac gacaaggcat gcgaggttga
      961 ggccgccgag acccacgagg tcccaggcgc cgagagtggg aggttcttcg tcaccggtca
     1021 tcacctgatc ggctcccgtc actgagcacg cgtgcgtacg tcgtttcggg gttccccgcg
     1081 cgggaggtgc cgacgctatc acagcggaac cgacgctcct cggccacgcc cacgacgcct
     1141 gctgccacgt cctccactgc ggatcggagg tacagaacat cgcttgtcat gctggggcgc
     1201 cgtcctcgat gtcggggtgg gtcccgggct cagtgtgctg tctggctgtg ctgtctgcct
     1261 gtgctgtcca atgtgctggt gaaactgctt gtgaaactta gcacaaagtc ctgagaggaa
     1321 cctaatcgcg aggctaacga cgccctctgc tcatggcaac gcgagatcgc tcggtggaca
     1381 gacggagagt gccgacgatc ggggttcgga ggcacggccg gggcacatcg cggccagagc
     1441 tgtgcctcct cgatcagcgg ggatccggcg gtcgagccgt tcgacaagct caggaaccac
     1501 ctgtcgagac cctggcgcaa cgtcgatcag gcgtcctcgg tcttgatgat gccgctgtgc
     1561 ccgggtacgc cgaacgtcag cagcagcgcc accaccgcca tcgagctcag cccgagtacg
     1621 gaccaccagc ccgagaacag gctgatcagc acgacgccga acgcgaccag acctgcccac
     1681 aggtacatca cgaggaccgc ccgacgctgg gtgtgcccga tctcgagcag ccggtggtgc
     1741 aggtgtttct tgtccgggga gaacggggag cgaccagccc tggtccggcg tacgacggcc
     1801 agcacgagat cggcgaacgg gacgacgagc accgcgaacg ggaggatcag cggcagcagc
     1861 gccggcaaca gcgaggcgtt ggcgccgaac agaccctggt cgatccggct ggtggggtac
     1921 tggcccgtca ggctgatcgc cgagcacgcc agcatgaagc cgatgaggag tgcgccggag
     1981 tcacccatga acatccgggc cgggaagaag ttgtgcggaa ggaacccgaa acacactccg
     2041 gccagcgccg ccgtgagcat cgcgaccgtc gacgcccggg tctggtcgtt gacgagcgcc
     2101 agcaggacgg cgtaggtgaa gaacgcgacc gcgccgatgc cgacgacgcc ggcggcgagt
     2161 ccgtcgagac cgtcgacgaa gttcaccgcg ttcaccgtgg tgacgatgat gatgacggtg
     2221 aggagtgccg actgcgagct gccgagcgag aacgccgaca tcgaccgttc accggggagg
     2281 ggcagccaca acagctggat cccctgcagc acgaccacgc cggcagcgac cacctcgcct
     2341 gcgagcttgg tgatggcgtc gagctcgaag atgtcgtcga tgaccccgac cagacagatg
     2401 acgaatccgc cgagcaagac cgcgcgggcg tcgtcgacga tgagctgccg cttgccgagg
     2461 aacggcagct gcgtcgcaac gaggtacgcc gcggcgagtc cggccatcat ggccaccccg
     2521 ccgaagtacg gaatcgggat ctcgtgcacg tcgcggtcgc ggacacgggc gtaggcgccg
     2581 aacttcatcg cgagctcgcg cgcgatgaca gccaacaggt acgtgaccga caacgcgacc
     2641 acgaagatca gcacgtactc gcgcatcagg cccgaccggt atcctggccg ccgtccgccg
     2701 agtcgtcgtc cgagccgtcc gagccgtccg agccgtccga gtcgtcagag tcgtcggcca
     2761 gcgcgtcggc gggcgcctcg aggtcggaca ccacttcgcg cagcgcgtga acactgacga
     2821 caccgacccg gacgacccgc agcgcgtcgc cggtgcagtc gacgatggtc gacggcaggc
     2881 tgcccggcgt tggtccgccg tcgaggtaga cgtcgacgga ctcaccgagc atgtcctgcg
     2941 cctgtcgagc ggtggtggcc ggcggctgac cggtgatgtt ggcgctgctg acggcgagcg
     3001 gcccgttgtc ggcgagcagg tcgagggcgt ccggttggtc aggcatccgc agcgcaacgg
     3061 tggagcggct gtcgccgagg tcccagcgca ggctgggctg ctgacggcat atcagtgtca
     3121 gccccccggg ccagaacgcc gacgcgagcg cccggacgtc cggggtcagg ttggtcgcca
     3181 acgcccgaag cgtgtcgttg ccaccgatga ggaccggcgt tggcatgttg cggccccgtc
     3241 ccttggcgcg cagcaggcga cgtacaccgg tcggatcgaa cgcgtcggcg gcgaggccgt
     3301 agacggtgtc ggtcgggaac acgacgaggc cgctgcgtcc gaacgcttct ttcgcctccg
     3361 cgaagcccgc gctgcgctgt gcgggatcgg ccaggtcgta gacgccgggc gcaggcgagc
     3421 tgtccggctc tgtcgtgctc acgcgcctat cgtgccaggc gacgtaccgt ccggacgtgc
     3481 ggcccgcggc acccgacgag cagtgacgaa ccggggtcgg cccgtcagat ccgggtggtc
     3541 gcggacgtcg caccacaacc cggagtcgac gaagacccgg ggggccgatt cgccctggac
     3601 gtcggcgtgc tcgcaggtga ccaggccacc gtcgacgagc agctcggcgg cctggtgcgc
     3661 caccagccgg atcgcgtcga gtccgtcgtc gccggaccac agcgccagcg ccgggtcgaa
     3721 atctcgcacc tcgacgtcca cgctttccca ggcctcaagc gggatgtacg gcggattggc
     3781 cgtgaccacg tgaaccgaac ccgccagatc ggccagactg cgcgcagcgt cggccaggtc
     3841 gcccacacgg atgtcgacgc cggtgccgcg ggcgttgcgc tcggcgtacg ccgcggcctc
     3901 cggtgacagc tcgatgccga cgacctgcgc ctgcggcacc tcggtgccga gcgcgatcgc
     3961 gacagccccg cagccggtgc agaggtcgac ggcccgcggc gcgacgccgg cgaccaccaa
     4021 ctgcctcagc tcgtcgaccg ccactcctgt catcacctcg gtctccggcc gcggcacgaa
     4081 gacgcccgga cctacctcga ggtcgaggta gcggaagccg acccgcccgg tgaggtgctg
     4141 caacggctcg cggccggctc gacggcggac aaggtggtgg aactgctcgc gctggacctg
     4201 tgtcacggtg ctgatgccgg ccagcgcggg tcgctgcgtg cgcatgacgt gcgcggccag
     4261 catctcggcg tcgtggcgag gtgacggcac tcccccgtcc gcaagcgtcc tggtcgcatc
     4321 ggcaaggagg gttcgaaggt cgtatgtcac ggtgccgact cgacggccgc caacttgtcc
     4381 gccaggtcgg cctggacgtc cgcgtcgatg accgcctgga ggtcaccgtc gaggacctgg
     4441 tcgaggttgt aggccttgta gccgacccgg tgatcggtga tccggttctc ggggaagttg
     4501 taggtgcgga tgcgctccga gcggtcgacg gtccggacct gcgaccggcg agcgtcggcg
     4561 ttctcggcat cccgctgttc ctgagcgacc tggagcagtc gcgaccggag cacacgcatg
     4621 gcctgctcac ggttctgcag ctggctcttc tcgttctgac aggacacgac gatgccggtg
     4681 ggcaggtgcg tgatgcggac ggccgagtcc gtggtgttga cgctctgccc gccgggacca
     4741 gacgacctga acacgtcgac acgaagatcg ttctcgtcga ccgtgacatc gagctgctcg
     4801 gcctcgggca tgaccaggac accggccgca gaagtgtgga tccggccctg cgactcggtg
     4861 accggcaccc gctggacgcg gtggaccccg ccttcgaact tgagcagcgc gtacggcgcc
     4921 tctcccggct ccggggtgcc cttcgccttg accgccaccg tcaccgactt gtagccgccc
     4981 aggtcggact cggtcgcgtc gatctgctcg gtcttccacc cccgccgctc ggcgtaccgc
     5041 atgtacatgc ggagcaggtc gccggcgaac aacgccgact cctccccgcc ctcgccggac
     5101 tt
//
LOCUS       DATBFZ010000149        11379 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_754091, whole genome shotgun sequence.
ACCESSION   DATBFZ010000149 DATBFZ010000000
VERSION     DATBFZ010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 11379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11379
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_754091"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(27..1862)
                     /locus_tag="VH419_04500"
     CDS             complement(27..1862)
                     /locus_tag="VH419_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042911.1"
                     /translation="MSRADAYRAFYRDSRARLLHQVYAYCGDTEVAQRALTDAFVSAG
                     HHWRKLSGDPGKDAWMRARAFRATRRGLNRARRPWYVRAGRTADGHRPLLVALHSLSP
                     TDRHLVVLVHLAGLDLLTAAREVGTTDGAAEQALSSAELVLAEHGVDGTDGIRAALFD
                     LRDDLSEGRMDSAARLRREGNRRRRARLGLAGLCSVALVIGAGALTAAQPEHLGSPGT
                     SDTAAGTQDDADVATRLDKIDASVLTATDDVRKLTSVHGWRLDGTSRDLRTSTPYDEC
                     LRAVPNDPRAAHFYVRTFRSGSGPRPTVATQAVEVSASVEAADSSYARLVKRFSACTA
                     DNHQITRYSTVRGVGDVSSVISLKYVDKRGVHDQLISISESGLATIVWVIDAPNDQPP
                     AARRVVTLAASSVRAVCRLSMGTCPRRPYLIVGQVPPKVDRADGFLTAIDLPVFAGLT
                     EPWVATSPNAVSSNPAATGCDRADFAGSKATTLSARSFVVPSARTLATIFGMTETRGV
                     FPSVARAENFLDDVSRNVTTCHDRMLSLQVLSTRSIDVEQGTARIWTIKQTMSRSRDL
                     TFRVALVRVGTTVAQVTFTPMPGFDLSPEQFASLARRAALRALQV"
     gene            2089..3363
                     /locus_tag="VH419_04505"
     CDS             2089..3363
                     /locus_tag="VH419_04505"
                     /inference="COORDINATES: protein motif:HMM:NF040070.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6350 family protein"
                     /protein_id="HEY7042912.1"
                     /translation="MRQTPVRGVSGSVTDATSATMNAMTDLMTRPVLRVNAATEPPRR
                     PIVVSAMVAGVAAALAGLVVSMSLSVGAWFDGDTGTFTDAVRVGAFGWLVSNGTGVTV
                     DETTFTAIPLGSLFLCGYAVYRAGRWVGATSAVRHIWDVVTGTVVLALAYAGTGLVVA
                     AVCRTGSASTGFVRTALTLTATGVAVGAVGVIRGSDSAGPVTALVPVPVRACLRGALA
                     GYAAMVAAAAAALVASLVVHFSSAVTLQEGLAAGTVGSIAVALICLASVPNAVLCAGA
                     YLAGPGFVLGSGTTVAPGDVELGRLPALPILAAIPTSGEPRWWDAVLICVPVLAGAVA
                     GLVAWRRDPVYGLDQAALRGAASAFVGGTAFGLSTWLATGAIGPGRMQDIGPNVLATT
                     AVCGLAFLVGGAVAAGAARWWQAARSGSARPL"
     gene            3448..4053
                     /gene="purN"
                     /locus_tag="VH419_04510"
     CDS             3448..4053
                     /gene="purN"
                     /locus_tag="VH419_04510"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012918935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEY7042913.1"
                     /translation="MPARLVVLASGTGTNLQALLDACADKDYGAEVVAVGSDRSATMA
                     VRRAQDAGIPTFELALSAYASRDEWDAALTKTVASYEPDLVVSAGFLKLVGATFLEEF
                     GGRFVNTHNALLPSFPGIHGPRDALAYGVKITGATLFVVDEGVDTGAIVAQVAVPVED
                     GDDEDALTERIKQAERAMLVHEIGRMAREGFTIDGRKVTIP"
     gene            4050..5621
                     /gene="purH"
                     /locus_tag="VH419_04515"
     CDS             4050..5621
                     /gene="purH"
                     /locus_tag="VH419_04515"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEY7042914.1"
                     /translation="MSSSPSNERRPIRRALVSVYDKTRLDELATALDAAGVTIVSTGS
                     TAARIAATGAAVTRVEELTGFPECLDGRVKTLHPTVHAGILADLRLESHRQQLADLGI
                     EAFDLVVSNLYPFRATVRSGADPDECIEQVDIGGPSMVRAAAKNHPSVAIVTSPEQYD
                     GVFEALVVGGFTLAQRQRFAAAAFAHTAAYDVAVADWFAGQVEAEAEQSWPEFLGRTY
                     RRQAVLRYGENSHQQAALYVDPDAEPGLAQAEQVHGKEMSYNNYVDAQASYRAAYDFA
                     EPAVAIIKHANPCGIAIGTDVADAHAKAHACDPVSAFGGVIATNRPVTAAMAAQVAEV
                     FTEVVVAPAYDDDALDLLTRRKNVRLLICGPPPTTVLETREISGGVLAQQPDRFQSPG
                     DDPATWQLVTGSQLDEAGLADLAFAWRACRSVKSNAILLAKNGATVGVGMGQVNRVDS
                     ARLAVARAGDRAEGSLAASDAFFPFPDGLEVLTEAGVRAVAQPGGSMRDDVVVEAAQA
                     AGVTMYFTGTRHFAH"
     gene            5762..6619
                     /locus_tag="VH419_04520"
     CDS             5762..6619
                     /locus_tag="VH419_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002532729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEY7042915.1"
                     /translation="MTAQTLDGKATAQAIRADLTRRVERLAEHGIRPGLGTVLIGDDP
                     GSVSYVNGKHRDCAQVGIASIREHHAAGISQAFLEERIAVLNADPACTGFIVQLPLPR
                     HLDAQRALELVDPMKDADGLHPMNLGRLVLMADAPLPCTPRGIVELLTAYDVKLAGAD
                     VCVVGRGVTVGRPLGLLLTRRTENATVTLCHTGTRDLAEHTRRADIVVAAAGVPRLIS
                     ADMLKPGCVVLDVGTSRTDAGLVGDVAPDAAEVASHLAPMPGGVGPMTRAMLLTNVVE
                     AAERAAGLA"
     gene            6630..6911
                     /locus_tag="VH419_04525"
     CDS             6630..6911
                     /locus_tag="VH419_04525"
                     /inference="COORDINATES: protein motif:HMM:NF022668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3017 domain-containing protein"
                     /protein_id="HEY7042916.1"
                     /translation="MNWLKKPSTVGGIIYLVVVAGVLAGLGIVAVGAWRPGIATMGAS
                     FGLALVSRAVLPDERAGMLRIRRRFVDLTALAICCGGMLGLAAVIPGLR"
     gene            7036..7875
                     /locus_tag="VH419_04530"
     CDS             7036..7875
                     /locus_tag="VH419_04530"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HEY7042917.1"
                     /translation="MRNKSASRAVLHQALKTARYEVLPTAKIEGVIVETVPRDITITV
                     TASPARGIEATLDLTTRLVGAGYHVVPHLAARMVHGKTELAEIVDRLRGIGVTNVFCP
                     AGDADPPAGEYVGSLPMLEHLAELGRPFPDVGITGYPESHPAIEDDVTIQSMWDKRTH
                     ATYVVSNLCFDAATIAVWLRRMRRRGIMLPVLIGLPGPVERTKLLTMAARVGVGQSMR
                     FVRSHASAFARIAAPGGFSPERFLIKAAPSLNDPELGVAGLHLFTFNQVAETEAWRQS
                     LLG"
     gene            7978..8358
                     /locus_tag="VH419_04535"
     CDS             7978..8358
                     /locus_tag="VH419_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042918.1"
                     /translation="MNTHHTSSSQKPAKSKLARVTLGLGLVASTLALAAGTETSASAS
                     SDATTAPASQWSRCFYSAHGYDAWTKKQVGMPRCDLLPAATAPLQLEGSATSSLGVDG
                     ACFKNPKYWTVTSKNGTFVCTPNR"
     gene            8580..8936
                     /locus_tag="VH419_04540"
     CDS             8580..8936
                     /locus_tag="VH419_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042919.1"
                     /translation="MYRHNTTHRSSAYRLGRGAIIAGGLVAVAFVGISGPATGSHTTH
                     DAPAHSVTNESTVTTPCFMVPTGDRWPAHEVGPVPRCEHTYGATEQGRAFQILSDQRN
                     TLSNHRNMPIGRVPLP"
     gene            complement(8973..9509)
                     /locus_tag="VH419_04545"
     CDS             complement(8973..9509)
                     /locus_tag="VH419_04545"
                     /inference="COORDINATES: protein motif:HMM:NF019413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1638 domain-containing protein"
                     /protein_id="HEY7042920.1"
                     /translation="MTRRAWPVDVHPLPPLLHNRPERIAGEVERLAVELAPQYQQLAV
                     AYADCGTYGALDDVCDRLHLRRLPGLHCYDVYAGADRLQRYFDDQPGTYLLTDFLARS
                     FARTVLTELGLDRYPELRETYFGHYTRVVWLAQDSDEETAALAAEAARAIGLPLTRIE
                     VGTAGLEHALESLVAEPC"
     gene            complement(9637..>11379)
                     /locus_tag="VH419_04550"
     CDS             complement(9637..>11379)
                     /locus_tag="VH419_04550"
                     /inference="COORDINATES: protein motif:HMM:NF025925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HEY7042921.1"
                     /translation="IDSTCGGHGTCKKCKVQILGGSVPVSPLDIRSFTTPELKAGWRL
                     ACLAQATADLQVDVPPLLTRPKAATVGVGRQVILRPAIQKRYVELSEPTLSDQTPDLA
                     RLLDAIDDLELRVDLHALRRLPAVARQADFKLTAVVVDDVLVDVEPGDTTTRRYAIAF
                     DLGTTTVVATLLDTSTGTPEAVASMLNKQQPFGADVITRISKAMIDPSTLGRLRDLAH
                     QTLDELTREVCAEASIEPAEIYEIAVAGNATMAALALGIDPEPLGVAPFIMSTATLPE
                     LLASDLGVNVHPRARAVVLPALGAYVGGDIVAGMLASGMDRDKRTRLFIDVGTNCEIV
                     LSDGDRILSTAAPAGPAFEGGAIRCGMRAAEGAIEVVTIEPEGGTVTCGVIDDVAPRG
                     LCGSGLVDAVAQLVDIGLLDASGRFVTDDEAATRFPQLADRLGDVNGERVFALAWHDD
                     GKHELADSVYLSQRDVRELQFAKAAISTGWTLLLHELGLDPSDVQQVLLAGSFGSYLS
                     PASAVRIGLVPRLPVLRIVSAGNVAGEGAKMTLLSMQERKGAHALLEEVTYVELSDRP
                     DFNDRFVDQLAFPG"
BASE COUNT         1726 a         3891 c         4023 g         1739 t
ORIGIN      
        1 agccccatga ggttctgaca agtcggtcag acctggaggg cgcggagggc ggctcgtcgg
       61 gcaagcgagg cgaactgctc cgggctcagg tcgaaccccg gcatcggagt gaacgtcacc
      121 tgggcaacgg tggtgccgac ccgcacgagg gccacgcgga acgtcaggtc ccgagagcgc
      181 gacatcgtct gcttgatggt ccagatgcgg gcggtgccct gttcgacgtc gatggagcgc
      241 gtcgacaaga cctggagcga cagcatccgg tcgtgacagg tcgtgacgtt cctgctcacg
      301 tcgtccagga aattctcggc gcgggcgacg ctcgggaaca cgccgcgcgt ctcggtcatc
      361 ccgaagatcg tggccagcgt gcgggcactg ggtacgacga agctgcgggc ggagagcgtc
      421 gtcgccttgc tccctgcgaa gtccgcgcgg tcgcagccgg tggctgcggg attgctggag
      481 accgcgttgg gcgacgtggc cacccacggc tcggtgagtc cggcgaacac ggggaggtcg
      541 atcgcggtca agaacccgtc ggcccggtcg accttcggcg gaacctggcc gacgatcaga
      601 tagggccgcc gcgggcaggt gcccatgctg agcctgcaca ccgctcggac cgacgaggcc
      661 gccaacgtca cgacgcgacg cgctgccggt ggctggtcgt tcggtgcgtc gatcacccac
      721 acgatcgtcg ccagacccga ctcgctgatc gagatcagct ggtcatggac gccacgcttg
      781 tcgacgtact tgagcgagat gaccgacgaa acgtccccga caccacgcac cgtgctgtag
      841 cgggtgatct ggtggttgtc cgccgtacat gccgagaagc gcttgacgag ccgggcgtac
      901 gagctgtccg cagcctcgac cgatgccgag acctcgaccg cctgtgtcgc gaccgtcggc
      961 cgcgggccgg atccggaccg gaacgtgcgg acgtagaagt gtgcggcccg cgggtcgttg
     1021 gggaccgcgc ggaggcactc gtcgtacggc gtcgacgtgc ggaggtcgcg cgacgtgccg
     1081 tcgagtcgcc acccgtgcac ggacgtcagc ttccgtacgt cgtcggtcgc tgtcagtacg
     1141 gacgcgtcga tcttgtccag ccgcgtcgcg acgtcggcgt cgtcctgcgt cccagccgca
     1201 gtgtccgaag ttcctggcga cccgagatgt tctggctgtg cggccgtcag cgctccggcg
     1261 ccgatgacca gcgccactga acacaggcca gccagtccca gccgtgctcg tcgcctccga
     1321 ttgccctctc gacgcagccg cgccgcgctg tccattcggc cctccgagag gtcgtcacga
     1381 aggtcgaaca gggcagcgcg gatcccgtcg gtgccgtcca cgccgtgttc ggccaacacc
     1441 agctcggcgc tcgacagggc ctgctcggct gcgccgtcgg tcgtcccgac ctcacgggca
     1501 gccgtcagca ggtcgagtcc ggcgagatgg acgagcacga ccaggtgccg atcggtcggc
     1561 gagagcgaat gcagggcgac gaggagcggc cggtgtccgt ccgctgttcg accggcgcgc
     1621 acgtaccacg gccggcgcgc ccggttgagg ccccggcggg tcgccctgaa cgcccgcgcc
     1681 cgcatccagg cgtccttgcc gggatcgccg gagagcttgc gccaatggtg gccggcagag
     1741 acgaaggcgt cagtgagtgc gcgttgtgcc acctcggtgt cgccgcagta ggcgtagacc
     1801 tggtgcagca gccgtgccct ggagtcgcgg tagaacgcgc ggtacgcgtc agctcgcgac
     1861 atggacctgc tcctgggacg gttcgtgggc gactggggcc gcgctccgag ggtggagggg
     1921 ctggatcgac ggggtcatcg cgctgcgtcg ggtgcgcaga cacacggtgg acccatcgac
     1981 ggtagacgat ccggctgcct catatccaac cgagccagcc gcaaccgcgc atgaggcgac
     2041 atcgtttcgc ccgccgggag gtcatgctcg gcgctgtgac cagggtcgat gcggcagacg
     2101 cccgtgcgcg gcgtgtccgg cagcgtgaca gacgccacgt cggcgacgat gaacgcgatg
     2161 acggatctca tgacgcgccc ggtgctgcgc gtgaacgccg ccaccgagcc gccgcgtcgc
     2221 ccgatcgtcg tctccgcgat ggtcgccggt gtcgccgctg ctctcgccgg cctcgtcgtg
     2281 agcatgtccc tgtcggttgg agcgtggttc gacggtgaca ccggaacgtt caccgacgcg
     2341 gtcagggtcg gcgccttcgg ttggctcgtc agcaacggga caggggtcac ggtcgacgag
     2401 accaccttca ccgcgattcc gctcggctcc ctgttcctgt gcgggtacgc cgtgtatcga
     2461 gctggtcgtt gggtgggagc cacctctgcg gtgcggcaca tctgggatgt cgtgaccggc
     2521 accgtggtgt tggcgctggc gtacgccggc accggtctcg tggtcgcggc ggtctgccgc
     2581 acgggttcgg cctcgaccgg gttcgtccgt actgcactga cgttgacggc gactggcgtt
     2641 gcggtcggcg cggtcggggt catccgaggt tcggactcgg cagggccggt gaccgcactg
     2701 gtccccgtac cggtgcgcgc ctgcttgcgc ggcgcactgg cggggtacgc cgcgatggtg
     2761 gcggccgcgg cggcggcgct cgttgcctcg ctggtcgtcc acttctcgtc cgcggtcacg
     2821 ctgcaggaag ggctcgccgc cggcaccgtc ggctcgatcg ctgtcgcgct gatctgtctg
     2881 gcgtcggtgc cgaacgcggt gctgtgtgcc ggggcatacc tggccggacc tggtttcgtc
     2941 ctcggctccg gtacgacggt cgcgcccggc gatgtcgagc tcggccggtt gccggcgttg
     3001 ccgatcctgg ccgcgatccc gacgtcgggc gagccgcgct ggtgggacgc cgtactcatc
     3061 tgtgtccccg tgctcgccgg agccgtggcc ggactcgtcg cctggcggcg ggatccggtc
     3121 tacgggctcg accaagcagc cttgcgcggt gcagccagtg cgttcgtcgg ggggacagcg
     3181 ttcgggctgt ccacctggct ggcgaccggc gcgatcgggc ccggccgcat gcaggacatc
     3241 ggccccaacg tgctggcgac caccgcggtc tgtggtctcg cgtttctcgt cggcggcgcc
     3301 gtcgcggccg gtgccgcccg ctggtggcag gcggcgcggt ccgggtcggc ccggccgttg
     3361 tgacgccggc gcgctgtcgg acccagcgct gtcggcccct gcgctgtcgg acccagcgct
     3421 gtcggaccct gcgctatcgt cggcccggtg ccggctcgcc tcgtggtcct cgcctccggg
     3481 acgggaacca atctccaggc cctgctcgac gcctgcgcag acaaggacta cggcgccgag
     3541 gtggtcgcgg tcggctccga ccggtccgcc acgatggcgg tccgccgcgc gcaggacgca
     3601 ggcatcccga ccttcgagct ggcgttgtcg gcgtacgcct cgcgtgacga atgggacgcg
     3661 gcactgacga agaccgtggc gtcgtacgaa cccgacctgg tcgtctcggc cggcttcctg
     3721 aagctggtcg gagcgacgtt cctcgaggag ttcggggggc gcttcgtcaa cacgcacaac
     3781 gcgctgctgc cgtccttccc cggtatccac ggtccgcgtg acgcgctggc gtacggcgtg
     3841 aagatcaccg gggcgacgct gttcgtcgtc gacgagggtg tggacaccgg cgcgatcgtg
     3901 gcgcaggtcg ccgtaccggt cgaggacggc gacgacgagg acgcgctcac cgagcggatc
     3961 aagcaggcag agcgagccat gctcgtccac gagatcggtc ggatggcgcg cgaagggttc
     4021 accatcgacg gcaggaaggt cacgatcccg tgagcagcag tccatcgaac gagcgccggc
     4081 cgatcagacg tgccctcgtc tcggtctacg acaagacgcg gctcgacgag ctcgcgaccg
     4141 cgctcgacgc ggccggcgtg acgatcgtgt cgaccgggtc gactgccgct cggatcgcgg
     4201 cgaccggggc ggcggtgacc agggtggagg agctgaccgg cttccccgag tgtctcgacg
     4261 gccgggtgaa gacgctgcac ccgacggtgc acgccggcat ccttgccgac ctccggctgg
     4321 agtcgcaccg gcagcagctc gcagacctcg gcatcgaggc gttcgacctc gtggtgtcga
     4381 acctctatcc gttcagggcc accgtgcggt ccggcgccga ccccgacgag tgcatcgaac
     4441 aggtcgacat cggcggcccg tcgatggtca gggcagcggc caagaaccat ccgagcgtgg
     4501 cgatcgtgac gtcacccgag cagtacgacg gcgtgttcga ggcgttggtg gtgggcggtt
     4561 tcacgctggc gcagcggcag cgtttcgccg ccgcggcctt cgcccacacg gcggcgtacg
     4621 acgtcgcggt ggcggactgg ttcgccggcc aggtggaggc cgaggcggag cagagctggc
     4681 cggagttcct cggccggacg taccggcggc aggccgtgct gcggtacggc gagaactccc
     4741 accagcaggc cgcgctgtac gtcgaccccg acgcggagcc aggccttgcc caggcggagc
     4801 aggtgcacgg caaggagatg agctacaaca actacgtcga cgcccaggcg tcgtaccgcg
     4861 cggcctacga cttcgccgag ccggcggtgg cgatcatcaa gcacgccaac ccgtgcggca
     4921 tcgcgatcgg cacggacgtg gccgacgcgc atgcgaaggc ccatgcgtgc gacccggttt
     4981 cggccttcgg cggagtcatc gcgacgaacc ggccggtcac ggctgcgatg gcggcgcagg
     5041 tcgccgaggt gttcaccgag gtcgtggtcg cgccggcgta cgacgacgac gcgctcgacc
     5101 tgctcactcg ccgcaagaac gtccggctgc tgatctgcgg accgccgccg acgaccgtcc
     5161 tggagacgcg ggagatcagt ggtggcgtgc tggcccagca gccagaccgc ttccagtcgc
     5221 caggggacga tccggcgacc tggcagctcg tcaccggctc gcagctggac gaggccgggc
     5281 tggcggatct cgccttcgcc tggcgggcct gccggtcggt gaagtcgaac gcgatcctgc
     5341 tggccaagaa cggcgccacc gtcggtgtcg ggatgggtca ggtgaaccgg gtcgactccg
     5401 cccgattggc ggtggcccgt gccggcgacc gggcggaggg ctcgttggcc gcctccgacg
     5461 cgttcttccc gttcccggac gggctcgagg tgctgaccga ggccggcgta cgcgcggtcg
     5521 cacagcccgg tggctcgatg cgcgacgacg tggtcgtcga ggccgcccaa gcggccggcg
     5581 tgaccatgta cttcaccggc acccgccact tcgcccactg acgctgtcct tgcctcccac
     5641 ccttccccgc gaattgcggg aggttccggg atacggcggc cgagaaagcc ggaacttccc
     5701 gcaattcgcg gggaagcggg tctcgaccca ggacggtcgg caggggtggc acaatcggcg
     5761 cgtgactgcg cagactctgg acggcaaggc gaccgcgcag gccatcaggg ccgacctcac
     5821 ccgccgcgtc gaacggttgg ccgagcacgg aatccggccc ggactgggta ccgtcctgat
     5881 cggcgacgac cccggcagcg tgtcctacgt caacggcaag catcgcgact gcgcacaggt
     5941 cggcatcgcc tcgatccgcg agcaccacgc cgccggcatc agccaggcgt tcctcgaaga
     6001 acgcatcgcc gtgctcaacg ccgacccggc gtgtaccggg ttcatcgtcc agctcccgct
     6061 gccacgccac ctggacgccc agcgggcact ggagctggtc gacccgatga aggacgccga
     6121 cggtctgcac ccgatgaatc tcggccggct cgtcttgatg gccgacgcgc cgctgccgtg
     6181 cacaccccgc ggcatcgtcg agctcttgac ggcgtacgac gtgaagctgg ccggcgccga
     6241 tgtctgcgtg gtcggccgcg gtgtcacggt cgggcgacca ctcggactgc tgctcacccg
     6301 gcgtacggag aacgccacgg tcacgctctg ccataccggt acgcgcgatc tcgccgagca
     6361 cacgcggcgg gccgacatcg tcgttgccgc ggccggcgtc ccgcgcctca tcagcgcgga
     6421 catgctcaag cccggttgcg tcgttctcga cgtgggcacg tcgcgcacgg acgcgggtct
     6481 cgtcggtgac gtcgcgccgg acgctgctga ggtggcgagt catctcgccc cgatgcccgg
     6541 cggcgtcggt ccgatgaccc gtgccatgct gctcacgaac gtggtcgagg ctgcggagcg
     6601 agccgccggg ctcgcgtgaa ccggtcgact tgaactggct gaagaagccc tcgacggtcg
     6661 gagggatcat ctacctggtg gtcgtggcgg gcgtgctcgc cggcctcggg atcgttgccg
     6721 tcggcgcctg gcggccgggg atcgccacga tgggggcgag cttcgggctc gccctggtca
     6781 gccgcgccgt gcttcccgac gagcgggcag ggatgctgcg catcagacga cgattcgtcg
     6841 acctcaccgc gctggccatc tgctgtggcg gcatgctcgg cctcgctgca gtcatccccg
     6901 gtcttcgctg acctcggctg gtgtccacaa cctggccgga accattgacg atcggtcgct
     6961 gctgagcctg tccgcgcgcg tcgcccgggg ctagagtgag ggcagtttgg tgcgtatgaa
     7021 ggaatgagtt gatcaatgcg caacaaatcg gcatcccggg ccgtgctgca ccaagcgctc
     7081 aagacggcgc ggtacgaagt gctccccacg gcgaagatcg agggcgtgat cgtcgagacg
     7141 gtcccgcgcg acatcacgat cacggtcacc gccagccccg cccgcggcat cgaggcaacg
     7201 ctcgacctga caacccgtct ggtgggagcc ggctatcacg tcgtcccgca cctcgcggca
     7261 cggatggttc atggcaagac cgagcttgcc gagatcgtcg acaggctgcg cgggatcggc
     7321 gtcacgaacg tcttctgccc ggcaggagat gccgatccgc cggccggcga gtacgtcggt
     7381 tcgcttccca tgctcgaaca cctcgctgag ctgggccggc cgttcccgga cgtggggatc
     7441 accggctatc ccgagagcca cccggccatc gaggacgacg tgaccatcca gtccatgtgg
     7501 gacaagcgca cccatgcgac gtacgtcgtc agcaacctgt gcttcgacgc ggccaccatc
     7561 gccgtgtggc tgcggcggat gcggcgccgc gggatcatgc tcccggtcct gatcggccta
     7621 cccggcccgg tcgagcgcac caagctgctc acgatggcgg cacgggtagg cgtcgggcag
     7681 tccatgcggt tcgtcaggtc gcacgcgtcg gcattcgcgc ggatcgccgc gcccggaggg
     7741 ttcagccccg agcggtttct gatcaaggcg gcgccgtcgt tgaacgatcc cgagctcggc
     7801 gtcgccggtc tccacctgtt caccttcaac caggtggccg agaccgaagc ctggcggcag
     7861 agcctgctcg gctgacgtcg gccgaggata aggagttcgc cggatggcat aggcctgtct
     7921 tacgaccaac ctcggggtgt cgcgcaccgc cacttcacct cgaccggaag acagatcatg
     7981 aacactcacc acacgagcag cagccagaaa ccagcgaagt cgaagctggc gcgcgtgacg
     8041 ctcgggctcg gcctcgtggc ctcgaccctg gcgcttgcag ccggcaccga gacgagcgca
     8101 tccgcctcgt ccgacgcaac gacggcacca gccagccagt ggagccgctg cttctacagc
     8161 gcacacggct acgacgcctg gaccaagaaa caggtcggga tgccgcgatg tgacttgctc
     8221 ccggcagcga ctgccccgct tcagctggag gggtccgcaa cgtcctcact cggcgtcgac
     8281 ggcgcctgct tcaagaaccc gaagtactgg acggtcacgt ccaagaacgg caccttcgtg
     8341 tgcaccccga accgctgacg ccgagacgcc gaaatcagca acgtcagagg cagcgaagtc
     8401 gccaagtccg acgtcgatga gcccgcccca tggcaggggg cgggctcatc gctgcgagcc
     8461 ggcagggaga ggtcgtcacc gggccgaaga taagacgttc gccagatgac gcaggtccgt
     8521 cttacgacga gtttcgaggt atcagcagca gcatcctcac ctcgaaccaa ggacagatca
     8581 tgtatcgcca caacaccacg catcgcagct cggcataccg tctcggccgt ggagcgatca
     8641 tcgccggcgg cctcgtcgcc gtcgcgttcg tcggtatctc aggtccggcg accggctctc
     8701 acacgaccca tgacgcgccc gcacactcgg tgacgaacga gagcaccgtc accaccccct
     8761 gcttcatggt gccgacgggc gaccgctggc cggcgcacga agtcggaccg gtcccgcgat
     8821 gcgagcacac gtacggcgcg accgagcagg gcagggcctt ccagatcctc agcgaccagc
     8881 gcaatacgct gagcaaccac cgcaacatgc cgatcggcag agtgccgttg ccctgagccc
     8941 ggcggcggtc ccggcgacgg ccaggaggtt cgtcagcagg gctcggcgac gagtgattcg
     9001 agcgcgtgct cgagtcctgc ggttccgacc tcgatgcggg tgagcgggag gccgatcgcg
     9061 cgagcagcct cggccgcaag cgccgcggtc tcctcgtcgg aatcctgggc gagccagacc
     9121 actcgggtgt agtgcccgaa gtaggtctca cgtagctcgg gatagcggtc gaggcccagc
     9181 tcggtcagga ccgttcgcgc gaaggaccgc gccaggaagt cggtgagcag ataggtaccc
     9241 ggctggtcgt cgaagtagcg ctgcagccgg tcggcaccgg cgtacacgtc atagcagtgc
     9301 agtccgggga ggcgtcgcag gtggagacgg tcgcagacgt cgtcgagggc accgtaggtg
     9361 ccgcagtcgg cgtacgccac cgcaagctgc tgatactgcg gcgcgagctc gaccgcaagc
     9421 cgttcgacct cacccgcgat tcgctccggg cggttgtgca ggagaggagg aagtggatgg
     9481 acgtcgacgg gccacgctcg ccgcgtcacg atctccttgc agggttggga cagcgcacca
     9541 cacgcgatga gcgcgacagc gccacgacgc cggacagttc gaggttccga ggcgtcggcg
     9601 agacccggga ctgcgggaag ggaggcatgc gccccgtcag ccggggaagg cgagctggtc
     9661 gacgaacctg tcgttgaagt ccggccggtc ggagagctcg acatacgtca cctcctccag
     9721 cagtgcgtgc gcacccttgc gttcctgcat gctgagcagc gtcatcttcg cgccctcgcc
     9781 tgcgacgttg ccggcgctca cgatacgcag cacaggcagg cgcgggacca ggccgatccg
     9841 taccgcgctc gccggtgaga ggtaggagcc gaacgaaccc gcgagcagga cctgctgtac
     9901 gtcggacggg tccagaccga gctcgtgcag gagcagcgtc cagccggtcg agatcgccgc
     9961 cttggcgaac tggagctcgc gcacgtcgcg ctggctcagg tagacggagt ccgccagctc
    10021 gtgcttcccg tcgtcatgcc acgcgagcgc gaagacgcgc tcgccgttga cgtcaccgag
    10081 cctgtcagcg agctgcggga accgcgttgc cgcttcgtcg tcggtgacga accgacctga
    10141 ggcatccagc agtccgatgt cgaccagctg cgccactgcg tcgaccaggc ccgaaccgca
    10201 cagccccctg ggagccacgt cgtcgatgac gccacacgtc acggtgccgc cctcgggctc
    10261 gatggtgacg acctcgatgg ccccctccgc tgcgcgcatg ccacaccgga tggctccgcc
    10321 ctcgaaggcc ggaccggccg gcgcggccgt cgacaagatc cggtcaccgt cgctgagcac
    10381 gatctcgcag ttcgtcccga cgtcgatgaa gagccgggtg cgcttgtcgc gatccatgcc
    10441 ggaggcgagc atgcccgcca cgatgtcgcc gcccacgtat gcgccgagcg ccggcagtac
    10501 gacggcccgc gcccgaggat ggacgttcac accgaggtcg gaggcgagga gttcgggcag
    10561 cgtggcggtg gacatgatga acggcgccac gcccagcggt tcgggatcga tcccgagcgc
    10621 caaggccgcc atcgtcgcgt tgccggcgac cgcgatctcg tagatctccg ccggctcgat
    10681 cgaggcttcc gcgcacacct cacgggtcag ctcgtcgagc gtctggtgcg cgaggtcacg
    10741 gagcctgccc agcgtgctcg ggtcgatcat ggccttgctg attcgcgtga tgacgtccgc
    10801 gccgaacggc tgctgcttgt tgagcatcga cgccacggct tccggcgtcc cggtcgacgt
    10861 gtcgagcaac gtcgcgacca ccgtcgtcgt accgaggtcg aacgcgatgg cgtagcggcg
    10921 cgtcgtcgtg tcacccggct cgacgtcgac caggacgtcg tcgacgacca cggccgtgag
    10981 cttgaagtcg gcctggcggg ccacggcagg gagccggcga agcgcgtgca ggtcgactcg
    11041 cagctcgagg tcgtcgatgg cgtcgagcag ccgggcgagg tcgggtgtct ggtcgctgag
    11101 cgtcggctcg gacagctcga cgtaccgctt ctggatggcc ggacgcagga tgacctgccg
    11161 accgacgccg acggtggcgg ccttgggccg ggtgagcagc ggaggtacgt cgacctgcag
    11221 gtcggcggtc gcttgagcga gacacgccaa ccgccagccg gccttcagct ccggggtcgt
    11281 gaacgatcgg atgtccagcg gcgagaccgg gacgctcccg cccaggatct gcaccttgca
    11341 cttcttgcag gtcccgtggc cgccgcaggt cgagtcgat
//
LOCUS       DATBFZ010000150         5535 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_2067751, whole genome shotgun sequence.
ACCESSION   DATBFZ010000150 DATBFZ010000000
VERSION     DATBFZ010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 5535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5535
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2067751"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            230..580
                     /locus_tag="VH419_04555"
     CDS             230..580
                     /locus_tag="VH419_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020392112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042922.1"
                     /translation="MASYSVNAKAVRHARELIDKRQYVLDSDWGEVQPAAEAQNTYLD
                     SHSWADYAAWHLGLTEGANDETKARYAFVYGDFRRVHRTALIACVYRASEWRHKEIEL
                     AAHDLLQHLDTTSG"
     gene            complement(585..965)
                     /locus_tag="VH419_04560"
     CDS             complement(585..965)
                     /locus_tag="VH419_04560"
                     /inference="COORDINATES: protein motif:HMM:NF023390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS/SEC14 domain-containing protein"
                     /protein_id="HEY7042923.1"
                     /translation="MIEVVTGLPPRVAGVVVHGDLSAEDYRDGIRPAIEAAFAAGAPV
                     RMMFVLDSDFDGFSGGAVWEDAKLGLSHLTGWERVAVVGDVSWVNQSVRLFGFLMPGE
                     VRTYPLAERAAAEAWVTEGLSTQP"
     gene            complement(1011..1763)
                     /locus_tag="VH419_04565"
     CDS             complement(1011..1763)
                     /locus_tag="VH419_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042924.1"
                     /translation="MVVTDQRPQRAGLGRDLRRAARQLAAVSVPAAILGVVVGGVGGR
                     VAMALLFATNQETKGAVSDDGFTIGQFTLSGTMNLLGTGVQLGLTAAVCYLALRGLLI
                     GPRWFQVASMAVGAGVVVAAIIVAPDGIDFTAFDPPLLPVGMFILLPVLYVALLALVA
                     ERCLADDSWFATADLRIVLVLGGLCWLAGGLIVIVLPIVAAVWLAWRWFTQRGVVERL
                     NWPAVGWVFRGFLAALFALAVSNLLSDVQAIT"
     gene            complement(1799..4969)
                     /locus_tag="VH419_04570"
     CDS             complement(1799..4969)
                     /locus_tag="VH419_04570"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY7042925.1"
                     /translation="MGTGLDVDIRMLGGFSVTVGHAVVPDDEWSRRGAAALVKLLALA
                     EGRRLHREQVFDALWPDVPIDVAGPRLHKAAHFARRALGDDRPTLRLQHNLVELFPDA
                     EVSIDALEFRRLAEAAVATASQADAERALQWYAGQLLPMDLYEPWTTQWRERLTVLHE
                     ELLRLTERWEELLRANPADEAAHLALATAYADRGDRRAALRQLERMDQALRRELGTVP
                     SEPARQLREQLSGETGRPTSTAAKRQTQALVGRRKIGDELRARLDRADTGRGSAMVLS
                     GPAGVGKTALLDLAASLAARRGWRIARGGASAVEGAWPYAPVLEAFADLCRGHPALLD
                     GLDDSFRMELERALGGEQVTWSGETAHQRLFVAAAELLRLAASGHGLLLVVDDVHEAD
                     EASLRLLHYLARCAVSEQVVIALAARPDARPTFNQMRESLVARGIGGVVEVSPLDEVA
                     TRRLVAKAFPEFDEDTVAEIWAVSHGLPFRALEAARSAEGGTNDVAVSGLPGDALAAF
                     RRVALIGSTFSTDELLAVSGVDEDDTYRHLEAALETSVVEPTDAGYRFRHELVRQALL
                     STFSAPERTRSGHEVAEQLAALGAPPGRVAHLFISAGHTVQAIPYALPAIETAGALGA
                     YRDGLALVDAVIDHATGVDRGHLLARRGDLLMALGDMEAVDAYRGALSITSGTEHRLV
                     RVRLARAATFQGDLDTAAAALSGLELRGDAADAPLLLARGNLAYFTGDVDAAWEAADA
                     ARNLLQPDDPWQMVDMVSLQGLIAHQRGEWFERFRQELRRTKGNPAIAAAVFDAHLCV
                     AEYLLYGPIPYDEVIELAEDLRRRAEHHYRALRGVAFATALIGEAALLKGDLDTAERE
                     LQEAADLHRELDAAAGEAHSLQRLAEVRVARGDRAGAVELLHKALPLARWSVISNHLL
                     QRIYGTMINAADDATLAVSVVEQAEAALGETDRCTFCDVMFAVPAAIAYADAGDVEQA
                     ERYLVEAKISGRRWEGTAWRAAVAEVRAHVAAARGDQDEFDRWIRTAADLFSAAGQPL
                     DANRCSEQANSGRPMTPVSGALPLGT"
     gene            complement(5010..>5535)
                     /locus_tag="VH419_04575"
     CDS             complement(5010..>5535)
                     /locus_tag="VH419_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042926.1"
                     /translation="RTLRTNAPSSTLLDRLFALAAPAHVERLAAATRVGKVRGWGGVP
                     GFVRNSWGPGWALVGDAGYFMDPLNAHGMSEALRDAELLSSAVLESISGARPESAALE
                     DYQRRRDALSAQLFAASDELATYEWNGRLVQPLLRRLSASMTDEVELLESLPAAPVVP
                     RSASRLADGRALSG"
BASE COUNT          930 a         1984 c         1832 g          789 t
ORIGIN      
        1 tcgacgccgc gctacgccca tgtgacggca actgcggcta cctcgaccca ccggtcgacg
       61 gctgtcagca gcccctggac atcgacgcgg atgcgaagtc ccacgtcgag ttcaccgtcg
      121 gcgaaccttg tgacatcagc tgagtgcagc cgtcggccgc ggtttgcggt acgaggggct
      181 ggacaccgtg tgacctgcgg gatcgccgtc ccgctgtgat gatgggcgca tggcgtcgta
      241 ctcggtcaac gcgaaggcag ttcgccatgc gcgcgagctg atcgacaagc gacagtacgt
      301 cctcgacagc gactggggcg aggtccagcc ggccgctgag gcgcagaaca cgtacttgga
      361 ctcacacagc tgggcggact acgccgcctg gcacctcggc ctcacggaag gcgccaacga
      421 cgagaccaag gctcgatatg ccttcgtcta cggtgacttc cgtcgtgtcc accgaaccgc
      481 gctcatcgcc tgtgtctatc gcgcctccga gtggcggcac aaggagatcg aactggcggc
      541 gcacgacctg ctgcagcacc tggacacgac gtccgggtga cgtcctaggg ctgcgtcgac
      601 aacccctcgg tgacccacgc ctcggctgcg gcccgttccg cgaggggata ggtgcgcacc
      661 tcgccgggca tcaggaagcc gaacaaccgc acactctggt tcacccagga cacgtcaccg
      721 accaccgcga cgcgctccca acccgtcaga tgtgacaggc cgagctttgc gtcctcccag
      781 acggcaccgc cgctgaaccc gtcgaagtcg gagtcgagga cgaacatcat ccgcactggt
      841 gctcctgcgg cgaacgcagc ctcgatcgcc ggccggatcc cgtcccggta gtcctcagcc
      901 gacaggtctc cgtgtacgac gactccggcc acccgaggcg gaaggccggt cacgacttcg
      961 atcatgtctg ctccatttcg aatcgagcac cggcgaaccg gcgcgtgggc tcaggtgatc
     1021 gcctggacgt cgctcaggag gttggacact gccaacgcga acagcgcggc caagaacccc
     1081 cggaagaccc agccgacggc tggccagttg aggcgttcga cgacgccgcg ctgcgtgaac
     1141 catcgccagg ccagccacac cgcggcgacg atcggcagga cgatgacgat caggcccccg
     1201 gccagccagc acagacctcc gagcacgagc acgatgcgca agtcggcggt cgcgaaccac
     1261 gaatcgtccg cgaggcaacg ctcggcaaca agggccagca gggcgacgta cagcacggga
     1321 agcaggatga acatgccgac cgggagcagc ggtgggtcga aggcggtgaa gtcgatcccg
     1381 tcgggagcca cgatgatggc cgcgaccacc acgccggcac cgaccgccat cgacgcgacc
     1441 tggaaccacc gaggaccgat cagcagcccg cgcagggcga ggtagcagac ggcggccgtg
     1501 agtccgagct gcacgccggt gccgagcaga ttcatcgtgc cgctcagggt gaactgcccg
     1561 atcgtgaatc cgtcgtcaga gacggcaccc ttggtctcct ggttggtggc gaacagcagc
     1621 gccatggcaa ctcggccgcc gactccgccg accacgacac cgaggatcgc cgccgggacc
     1681 gagacggccg ccaactgccg cgctgcccgc cggagatcgc gccccagacc tgcacgctgt
     1741 gggcgttgat ccgtgacgac catccacgtc ctcctgtcga cccgtcagcc tacgggcatc
     1801 acgtcccgag cggcagcgcg ccggacacgg gtgtcatggg tctaccactg ttggcctgct
     1861 cggagcagcg atttgcgtca agtggttgcc cggccgcact gaacaggtcg gcggcggtcc
     1921 ggatccaccg gtcgaactcg tcctggtctc cacgcgcggc agcgacgtgc gcacgcacct
     1981 cggccacggc agctcgccat gccgtgccct cccaccggcg gcctgagatc ttcgcctcga
     2041 cgagataacg ttccgcctgt tccacgtcac ctgcgtcggc ataggcgatg gccgcaggta
     2101 cggcgaacat gacgtcacag aacgtgcagc gatccgtctc cccgagcgcc gcctcggctt
     2161 gttcgaccac gctcacggcg agggtcgcat cgtcggcggc attgatcatc gtgccgtaga
     2221 tgcgctggag caggtggttg ctgatcaccg accagcgtgc cagcgggagc gccttgtgca
     2281 gcagctcgac cgcccccgcg cggtcgccgc gagcgacacg gacctcggca aggcgctgca
     2341 gactgtgcgc ctcgccggcg gcggcgtcga gctcacgatg caggtcggct gcttcctgga
     2401 gctcccgctc cgcggtgtcg aggtctccct tcagcagcgc cgcctcgccg atgagcgcgg
     2461 tggcgaaagc gacgccgcga agcgcacggt agtggtgctc ggcgcgacga cgcaggtctt
     2521 cggcgagctc gatcacctcg tcgtacggga tgggaccgta gaggaggtac tcggcaacgc
     2581 acagatgcgc gtcgaagact gcggcggcga tggcagggtt gcccttggta cgccgcagct
     2641 cctgccggaa ccgctcgaac cactcacccc gctggtgcgc gatcagccct tgcagcgaca
     2701 ccatgtccac catctgccag gggtcgtccg gctgcagaag gttccgcgcc gcgtcagccg
     2761 cctcccaggc ggcatcgacg tctccggtga agtaggcgag gttgccgcgg gccaggagca
     2821 gtggtgcatc tgcggcatcg ccacgaagct ccaggcccga cagcgccgcc gcagccgtgt
     2881 cgagatcgcc ttggaaggtc gcggcccgtg ccagcctgac tcggacgagt cggtgctcgg
     2941 ttccgctggt gatcgacaat gcaccccggt aggcgtcgac ggcctccatg tcgcccagcg
     3001 ccatcagcag atctccacgt cgcgccaaca ggtgcccgcg gtcgacgccg gtcgcatggt
     3061 cgatgacggc atcgacgagc gccaacccgt cccggtaggc gcccagcgcc ccggccgtct
     3121 cgatggccgg caacgcgtac gggatcgcct gcacggtgtg accggcggag atgaacagat
     3181 gcgcgacccg gccaggaggt gcgccgagtg cagccagctg ctcggcgacc tcatgacctg
     3241 agcgcgtccg ctccggggcg gagaacgtgg acagcagcgc ctggcgcaca agctcgtgcc
     3301 ggaagcggta accggcgtct gtcggctcca ccaccgatgt ctccagggct gcctccaggt
     3361 gccggtacgt gtcgtcctcg tcgactcccg agacggccag cagctcgtcg gtgctgaagg
     3421 tcgacccgat gagtgcgact ctcctgaacg cggcgagggc gtcgcccggt aggccggaca
     3481 cagcaacgtc gttcgtgcca ccctcggcgc tgcgggccgc ctccagggcg cggaacggca
     3541 acccgtgcga aacagcccag atttcggcga ccgtgtcctc gtcgaactcg ggaaacgcct
     3601 tcgcgaccag ccgacgtgtc gctacctcgt cgagcggcga gacctccacg acgccaccga
     3661 tcccacgcgc cacaaggctc tcgcgcatct ggttgaaggt cggcctggcg tccggacgag
     3721 cagccagtgc gatcacgacc tgttcgctga cagcgcaccg ggcgaggtag tgcagcagac
     3781 gcaacgaggc ctcgtcggcc tcgtgcacgt cgtcgacgac cagcagcagc ccgtgtccgc
     3841 tcgcagcgag ccgcagcaac tcggcggcgg ccacgaacag ccgctggtgc gccgtctcac
     3901 cgctccacgt gacctgctca ccaccgaggg ctcgctccaa ctccatccgg aagctgtcgt
     3961 ccagcccgtc gaggagcgca ggatgccccc ggcacaggtc cgcgaaggcc tccagcaccg
     4021 gtgcgtacgg ccatgcaccc tcgacagctg aggcgccgcc acgggcgatc cgccagcctc
     4081 gacgagcggc cagcgaggcg gccaggtcca acagcgccgt cttcccgaca ccagccggtc
     4141 cggacaggac catcgcgctg ccacggccgg tgtcagcgcg gtcgagccgg gcgcgcagct
     4201 cgtcgccgat ctttcgccgt ccgaccagcg cctgtgtctg ccgtttcgcc gcggtggacg
     4261 tgggtcgccc agtctctccg gacagctgct cgcgcaactg ccgtgccggc tcgctcggca
     4321 cggtgccgag ctcgcgtcga agggcctggt ccatccgctc cagctgtcgc agcgcggcgc
     4381 gacggtcacc tcgatcggcg tacgccgtgg cgagtgccag gtgggctgct tcgtcggccg
     4441 ggttggcgcg cagcagctcc tcccatcgtt cggtcagccg cagcagctcc tcgtgcagga
     4501 cggtcagcct ctcccgccat tgggtcgtcc acggttcgta gaggtccatg ggcaggagct
     4561 gaccggcata ccactgcagc gcccgttcgg catcggcttg cgacgcggtg gccaccgcgg
     4621 cctcggcgag gcgcctgaac tccagcgcgt cgatcgacac ctcggcgtcc ggaaacagct
     4681 ccaccagatt gtgctgcagt cgcagtgtcg gcctgtcgtc accaagagcc cggcgggcga
     4741 agtgggcagc cttgtgcagg cgcggtccgg ccacgtcgat ggggacgtcc ggccagagcg
     4801 catcgaacac ctgctcgcga tggagccggc gaccctcggc aagggcgagc agcttcacca
     4861 gtgccgcggc tccacgtcgg ctccactcgt cgtccggtac gacggcatgt cccaccgtga
     4921 cggagaaccc gccgagcatt ctgatgtcga catccaaacc ggtgcccaca cgccctccca
     4981 ggaccggcgc acgatcagat gcggtcacgc taccccgaga gcgcacgacc gtcagccagc
     5041 cgagacgcac tgcgcggcac gaccggcgcg gcggggagcg attcgagcag ctccacctcg
     5101 tcggtcatcg aggcactgag ccgacgcagc aacggctgga cgagacgacc gttccactca
     5161 taggtcgcga gctcgtcgct ggctgcgaac agctgtgccg acagcgcatc acgccgacgc
     5221 tggtagtcct cgagcgcggc ggactcggga cgggctccgg agatggattc caggactgcg
     5281 ctcgacaaga gctcggcatc gcgcagggcc tcgctcatcc cgtgtgcgtt caacgggtcc
     5341 atgaagtagc cggcgtcacc gaccaaggcc caaccgggcc cccatgagtt ccggacgaag
     5401 ccgggtacgc cgccccagcc acgcaccttg ccgacacggg ttgccgcagc gagccgttcg
     5461 acgtgcgcag gggctgcgag ggcgaacagc cggtcgagca gcgtgctgct cggcgcgttc
     5521 gtccgcagcg tgcgc
//
LOCUS       DATBFZ010000151         7481 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_755534, whole genome shotgun sequence.
ACCESSION   DATBFZ010000151 DATBFZ010000000
VERSION     DATBFZ010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 7481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7481
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_755534"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..548
                     /locus_tag="VH419_04580"
     CDS             <1..548
                     /locus_tag="VH419_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011753508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HEY7042927.1"
                     /translation="SMSVIRAAQVAAEAAAGLSEDAEDAPVVEATAEEVREVKPQYVF
                     TITDGGFAKRTRISEYRLTNRGGLGVRAMALANEDRGGLVGAFIVEEGDEVLSITQAG
                     QVVRSPINDQFRPTGRSTMGVRFVTPKEGDAVAVVARSVEAREEEQTVEESSDAGSDA
                     TIEATDDPADDPVDPETDREE"
     gene            555..1118
                     /locus_tag="VH419_04585"
     CDS             555..1118
                     /locus_tag="VH419_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017933483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3566 domain-containing protein"
                     /protein_id="HEY7042928.1"
                     /translation="MTDRSVEHTVTRTGGERSRSEDTAVRAPLGQRISARLSNAADEH
                     RANAAPTAPPRPAKKAGRRPPRRARLRLTRIDPWSVMKTSFLLAIAFGVVTVVSVFII
                     WSVLAAAGVWDSINQTVQDVVGGEDAAGWDVEKYVGISRVMGFTILVSVVDVILITAI
                     ATLGAFLYNMSAALLGGVELTLAEDER"
     gene            1177..1253
                     /locus_tag="VH419_04590"
     tRNA            1177..1253
                     /locus_tag="VH419_04590"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1211..1213,aa:Ile,seq:gat)
     gene            1299..1424
                     /locus_tag="VH419_04595"
     CDS             1299..1424
                     /locus_tag="VH419_04595"
                     /inference="COORDINATES: protein motif:HMM:NF038356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DLW-39 family protein"
                     /protein_id="HEY7042929.1"
                     /translation="MKKLVLVLLAVGGAVFAKKKFDESKKEQALWAEATDTVDRS"
     gene            1600..1672
                     /locus_tag="VH419_04600"
     tRNA            1600..1672
                     /locus_tag="VH419_04600"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1633..1635,aa:Ala,seq:tgc)
     gene            1772..2161
                     /locus_tag="VH419_04605"
     CDS             1772..2161
                     /locus_tag="VH419_04605"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HEY7042930.1"
                     /translation="MSKEIPVVGAIIERDGLILCARRPHRGSIGGLWEFPGGKIERGE
                     TARVALEREIREELGCHIEVGDEVTTTRHEYDFGIVRLTTFRCHLVDGTPQLTEHAEI
                     RWVSPAQLPALGWAPADIPAVQIVSAA"
     gene            2158..5040
                     /locus_tag="VH419_04610"
     CDS             2158..5040
                     /locus_tag="VH419_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3427 domain-containing protein"
                     /protein_id="HEY7042931.1"
                     /translation="MSLDPVWPSQFQLDIDFGYVASAAVAPRRYNPRLVVNADGSTVE
                     HALLEELRRCEAFTFSVAFVSPSAIAQLKQHLYDFSGRGRIITSDFLAFNQPETFSEL
                     LRLREHGGIDVRRHTARGFHPKGYIFEQGPSVTAMIGSSNLTSQALSRNHEWNLKVSA
                     ARGSDLADQIDRLLEEQVVGSEPLTEEWIEEYADAYVAPPRRGPVPVLPEPAETPVVV
                     HPNAMQQDALLALDLARAEGARRAIVISATGTGKIMLSALDVRAVAPKRMLFIAHREQ
                     ILDRTIEEYRRVLGGPPSDYGKLTGHARDGDRRYVFATIQTLSQLHVLAAVASDSFDY
                     VIIDEAHRAGSATYQRVIDRLTPGFLLGMTATPERTDGFNVFELFDYCVPYEIRLNHA
                     LEAEMLSPFHYYGVSDITYDDGRTTSDKTDLSLLIRPERVRFLVEVLEKYGQARVAPC
                     GLIFCSRKEEAHALAAALTDCELNGRPLRTVALTGDDSIAVREQRVTELQAGDLDYLL
                     TVDIFNEGVDIPQVNQVVMLRQTQSAIVFVQQLGRGLRLAEGKDYLVVIDVIGNYTNN
                     FLIPIALFGDESLNRESLREKLNETVECSVLPGHSSVSFDEVSRDRILRSIEATSLDS
                     MRRLRSALEAMQHRVGGVPKLWDFHRFESVDPVLLATKVEHYPGLVQRLLRVDAELSA
                     SASRALSLLSHEVLAAKRLHEYVLLRLLRGRESVTRSEIATAFADAGLASDAGAVHAA
                     LATLTLRGYAASDLKRYVDPLVTIEDDDVRLTEAFREDLASSERLRREVDDLLITGET
                     LTRSRYRIDRPFTTGLQYSRRDAARIIGWPRSTSSTIYGYKTDRSRDVCAVFVTLHKS
                     EHIDASTAYEDALLDETSMRWFSRSRRTLATDEVKAIVDGDVDVHVFVKKDDVESDHY
                     YLGVATAHDSVQTTMPDGNGADLPVVTMTLRFAEPINQGLFDYFRP"
     gene            complement(5194..6243)
                     /locus_tag="VH419_04615"
     CDS             complement(5194..6243)
                     /locus_tag="VH419_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011751449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HEY7042932.1"
                     /translation="MSELVPGDDVPDDEKALLDYLVGIVEHGRRVASVQVNSTLTITY
                     WLVGRAISINALSSRRADYGREILGTLSQELGARFGAGFDSTNLSRMVAFARMYPDFD
                     QTTSLAHQLSWSHVRALLALKSDEARRFYAEEAASKRLSVRELRAAIARKAYERREIA
                     NSQIPEGSAMPRDTFRDPLILDTLGLHDSYLERDLEAAILRGVQDFLLEVGNGFAFVA
                     SQKRMVIDGDDFHLDLLFFSRPLRRLVAVELKIGKFDARFEGQMKLYLKWLDRYDRQA
                     GEEAPIGLILCTEVSREQVELLEMHKDGIVVAEYWTALPPKAELEAKLAEIVRDAQER
                     LARRGLPAAPADATA"
     gene            6395..7111
                     /locus_tag="VH419_04620"
     CDS             6395..7111
                     /locus_tag="VH419_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042933.1"
                     /translation="MLIDTTFDVRTDAGGKDPDTWSPTLCRYHQFLWSKPLPSGVCFE
                     LTPTSRPPYYFRHTSQVGDFALSSDAFIPAYTRYGVPKSVIEQLPLGEHVHFNTIGYT
                     IGGLILWPANRIDGKWTINQARGCLRRTIGDRMDPTLECVRRHYSGEKSPLGDVLARY
                     ADFFAAFEDFRGYVDFWLLRDLVTDDHSAVRFFTPFDDFRTPAIPPDLDSYVAYRRCS
                     IEFLEARNARIARATRDSRM"
     gene            7221..>7481
                     /locus_tag="VH419_04625"
     CDS             7221..>7481
                     /locus_tag="VH419_04625"
                     /inference="COORDINATES: protein motif:HMM:NF014193.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family cytochrome oxidase biogenesis
                     protein"
                     /protein_id="HEY7042934.1"
                     /translation="MPRLLAPRLWGAHLLALVLVGAAFGLGLWQYGAWQDHRDAEAVD
                     LTHAKPEPLDDVIGPDDPFPGDKIGQPVTVDGTWVPDGTVYVS"
BASE COUNT         1288 a         2443 c         2455 g         1295 t
ORIGIN      
        1 tgtcgatgtc ggtgatccgc gcggcccagg tcgccgcgga ggcggccgcc ggactgagcg
       61 aggacgccga ggacgcgccg gtcgtcgagg cgaccgccga ggaggtccgc gaggtcaagc
      121 cgcagtacgt cttcacgatc accgacggcg gcttcgccaa gcgcacccgg atcagcgagt
      181 accgcctcac caaccgcggc ggactcggcg tacgcgcgat ggcgctggcc aacgaggacc
      241 gcggcggcct ggtcggcgcg ttcatcgtcg aggagggcga cgaggtgctc tcgatcaccc
      301 aggccgggca ggtggtccgc agcccgatca acgaccagtt ccggccgacc ggccgctcga
      361 cgatgggcgt gcggttcgtc acccccaagg agggcgacgc ggtggccgtc gtggcccgct
      421 cggtcgaggc gcgcgaggag gagcaaacgg tcgaggaatc gtccgatgca ggctcggatg
      481 caacaatcga agcgaccgac gatccagccg acgatccagt cgaccccgag accgaccggg
      541 aagagtgagg cccgatgacc gaccgttccg ttgagcacac cgtgactcgc acgggcggcg
      601 agcgctcccg cagcgaggac accgccgtac gcgcccctct cggccagcgg atctcggccc
      661 ggttgagcaa cgccgccgac gagcaccgcg ccaacgctgc gccgacggcg ccgccgcgcc
      721 cggccaagaa ggccggccgg cgccccccgc gccgggcccg cctgcgactg acccggatcg
      781 acccgtggtc ggtgatgaag acgtcgttcc tgctggcgat cgccttcggc gtggtcaccg
      841 tggtgtcggt gttcatcatc tggtcggtgc tcgccgccgc cggcgtgtgg gactcgatca
      901 accagaccgt ccaggacgtc gtcggcggtg aggacgccgc cggctgggac gtcgagaagt
      961 acgtcggcat ctcccgggtg atgggcttca ccatcctcgt ctcggtagtc gacgtcatct
     1021 tgatcaccgc catcgcgacc ctcggcgcgt tcctctacaa catgtccgca gccctcctcg
     1081 gcggcgtcga gctgaccctc gccgaggacg agcggtgagg cgctctatcg ttttgtccgc
     1141 tcactccgcg gtggggtagc cttcccccgg ttctgcgggc ctatagctca gacggttaga
     1201 gcgcttccct gataaggaag aggtcacagg ttcaagtcct gttaggccca ccatcctcgc
     1261 gccaccaccc ggcaacaccg agaccgtggg agtccaccat gaagaagctc gtcctcgtcc
     1321 tgctcgccgt cggcggcgcc gtcttcgcga agaagaagtt cgacgagagc aagaaggagc
     1381 aggccctctg ggccgaggcg accgacaccg tcgaccgctc ctgacccagc cctgcggggc
     1441 cgcggtcgtc gagcggtagt tccgcggtcg agccgcctct cggtggtcga gggataattc
     1501 ggcggtcgag ccgcctgtcg gtggtcgagg gataattcgg cggtcgagcc gcctgtcggt
     1561 ggtcgagcgg tctcccggtg gtcgagcttg tcgagaccag gggccttggc gcaattggta
     1621 gcgcacctgc tttgcaagca gggggttagg ggttcgagtc ccctaggctc caccctaggc
     1681 tccacgcgat aaagccgcag ggcagcctgg ttgaacgccc gctgcgcacg tggtgtcttt
     1741 attaagctcg ccctgacttc gggaggtgcg agtgagcaag gagatccccg tcgtaggggc
     1801 gatcatcgag cgcgacggct tgattctgtg cgcccggaga ccccatcgcg gatcgattgg
     1861 cggattgtgg gagtttccag gcgggaagat cgaacgcggc gagactgcgc gagtagccct
     1921 cgagcgggag atccgcgagg agttggggtg tcacatcgag gtcggagacg aggtgaccac
     1981 tacccgtcat gagtacgact tcggcatcgt gaggttgacg accttccgtt gccatctcgt
     2041 cgacggcacc ccgcagctca ccgaacacgc agagatccgt tgggtgtcac ctgctcagct
     2101 gcccgccctg ggttgggcgc cggcagacat tccggcagtc cagatcgtgt cagccgcatg
     2161 agcctggatc cagtctggcc tagccagttc cagctcgaca tcgactttgg gtacgtcgcc
     2221 agcgcagctg tcgctccgcg gcggtacaac ccgcgcttgg tggtcaacgc cgatggctcc
     2281 acggtcgaac acgccctcct cgaggaactg cggcgctgtg aggccttcac cttctctgtc
     2341 gccttcgtct cgccgagcgc aatcgcgcag ttgaagcagc acctctatga cttttccggc
     2401 cggggccgga tcatcacctc ggacttcttg gccttcaacc aacccgagac gttttctgag
     2461 ctgctcaggc tccgcgagca cggcggcatc gacgtacgcc gccacacggc acgcggcttc
     2521 cacccgaagg gctacatctt tgagcagggg cctagcgtga ccgcgatgat cggcagctcc
     2581 aacctcacca gccaggcgtt gtcgcgcaat cacgaatgga acctcaaggt ctcggccgcg
     2641 cgcggaagtg atctcgccga ccagatcgac agactgctcg aggagcaggt cgtgggttca
     2701 gagccactga ccgaggagtg gatcgaggag tacgccgatg cctacgtcgc acccccgcgg
     2761 cggggcccgg tgccggttct ccctgagccg gcggaaaccc cggttgtcgt gcatccgaat
     2821 gcgatgcagc aggacgccct gctcgccctg gacctggcgc gggcagaggg tgcccgtcgg
     2881 gcgatcgtca tctctgcgac cgggacaggg aagatcatgt tgtcggcgct cgacgtgcgt
     2941 gccgtagcac cgaagcggat gctcttcatc gcccaccgcg agcagatcct cgaccgcacc
     3001 atcgaggagt accgacgagt gctgggcggg ccgccgagcg actacggcaa actcactggt
     3061 catgcccgcg acggtgatcg gcggtacgtc ttcgcgacga tccagacctt gtctcaactg
     3121 catgtcctcg ccgctgttgc gtctgattcg tttgactatg tgatcatcga cgaggcccac
     3181 cgtgccggat cggcgacgta ccagcgcgtc atcgatcggc tgacgccggg attcctgctt
     3241 gggatgaccg ccacgcccga acgcaccgac gggttcaacg tcttcgagct gttcgactac
     3301 tgcgtgccct atgagatccg gctcaatcat gcactcgagg ccgagatgct cagtcctttc
     3361 cactactacg gcgtcagcga catcacctac gacgatggcc gcacaaccag cgacaagacg
     3421 gatctgagct tgctgatcag gcccgagcga gttcggttcc ttgtcgaggt actggaaaag
     3481 tacgggcagg cgcgcgtcgc gccgtgcgga ctgattttct gcagccgcaa ggaagaagca
     3541 cacgcgctcg ccgcggccct caccgattgc gagctcaacg gccggccgct tcgaacggtt
     3601 gcgttgaccg gcgacgactc gatcgccgtt cgtgagcagc gggtgaccga actccaggcc
     3661 ggcgacttgg attacctcct gaccgtcgac atcttcaacg aaggcgtcga cattcctcag
     3721 gttaatcagg tcgtcatgct gcgccagacc caatcggcga tcgtgttcgt ccagcagcta
     3781 ggtcgcgggt tgcggctcgc ggaaggcaag gactatctcg tcgtcatcga tgtcatcggc
     3841 aactacacca acaacttctt gatccccatc gcgctcttcg gtgacgagtc actcaatagg
     3901 gagtcgctgc gggagaagct caatgagaca gtcgagtgca gcgtgctacc tgggcattcc
     3961 agcgtgagct tcgacgaggt ctcccgtgat cggatcctgc gctcgatcga ggccacatct
     4021 ctcgacagca tgcgacgact taggtcggcg ctcgaggcga tgcagcaccg cgtcggcggt
     4081 gtcccgaagc tctgggactt ccaccgattc gagtcggtcg atccggtatt gctggcgacc
     4141 aaggtcgaac attacccggg cctggttcag cggctgttgc gagttgacgc cgagctgtca
     4201 gcgagtgctt ctcgcgcatt gagcctcctg tcccacgagg tcctcgccgc caagcggctg
     4261 cacgaatacg tcctgctgcg gctgcttcgc ggacgagaga gtgtcactcg gtccgagatc
     4321 gcgaccgcct tcgctgacgc gggactggcg agcgatgcgg gcgctgtgca cgctgcgctg
     4381 gccacgctca cccttcgcgg atacgccgcc tccgacctaa agcggtatgt agatccgctg
     4441 gtcacgatcg aggatgacga tgtgcggctg accgaggcgt ttcgtgaaga tctcgcctcc
     4501 tcggagcggc tccggaggga ggtcgacgac ctgctcatca ccggcgagac tctcacacgc
     4561 agtcgctatc gcatcgaccg cccattcacg accggcttgc agtattcgcg gcgcgatgct
     4621 gctcggatca tcggctggcc gcggagcact tcctcaacga tctacgggta caaaaccgat
     4681 cgaagccgcg acgtgtgcgc tgtgttcgtg actctccaca agtccgagca cattgacgca
     4741 agcacggcct acgaggacgc cctgctcgac gagacgagca tgcggtggtt ctcgcgatcc
     4801 cggcgcacgt tggccaccga tgaggtgaag gcaatcgtcg acggggacgt cgacgtacac
     4861 gtctttgtga agaaggacga cgtcgaatcg gatcactact acctcggcgt cgccaccgct
     4921 cacgactcgg tccaaaccac gatgccagat gggaatgggg cggacttgcc ggtcgtcacg
     4981 atgaccctcc gattcgccga gccgatcaat caagggctct tcgactactt ccgcccgtga
     5041 tggcatcgcc ggccccgcga tgacaaccag gacctgcctg gagcgcgccc cgcattccct
     5101 gcctcaggtt gggcagagct cccggcgatc ggcgggtttg catgggtgct ggtttggcag
     5161 ccattaatgc gtgcttcgaa ggggtggatg acgctacgcc gtggcgtcgg caggcgcggc
     5221 aggtaggccg cggcgcgcga gtcgctcctg cgcgtcgcgc acgatctcgg cgagcttggc
     5281 ctccaactcg gccttgggtg gcagggcggt ccagtactcg gccacgacga tgccgtcctt
     5341 gtgcatctcg agaagctcga cctgctcgcg gctgacctcg gtgcacagga tcaatccgat
     5401 cggagcctcc tcacccgcct gacggtcgta gcggtcgagc cacttgaggt agagcttcat
     5461 ctggccctcg aaccgagcat cgaacttccc gatcttgagt tcgacggcga ccagccgacg
     5521 taggggccgg ctgaagaaga gcaggtcgag atggaagtcg tcgccgtcga tcaccatccg
     5581 cttctggctg gcaacgaagg cgaagccatt gccgacctcg agcaggaagt cctggacgcc
     5641 gcgcaggatc gcggcctcca ggtcccgctc caggtagctg tcgtggaggc caagggtgtc
     5701 gaggatcagc gggtcgcgga aggtgtctcg cggcatcgca gagccctcag gaatctgcga
     5761 gttcgcgatc tcacgccgct cgtacgcctt gcgggcgatt gcggcgcgga gctcgcgaac
     5821 actgaggcgc ttggaagcag cctcctcggc gtagaagcgg cgagcctcgt ccgacttgag
     5881 cgccaggagt gcccggacgt gggaccaact aagttggtgc gccaatgagg tcgtctggtc
     5941 gaagtccgga tacatcctcg cgaaggccac catccggctg agattggtcg agtcgaaccc
     6001 ggctccgaac cgtgctccca attcttggct cagagtgcca agaatctcgc ggccgtagtc
     6061 ggcgcgacgg ctgctcaggg cattgatcga aatcgcgcgg ccgacgagcc agtaggtgat
     6121 ggtcagcgtg gagttgacct gcaccgacgc gacccgtcga ccgtgctcga cgatgccgac
     6181 gagatagtcg aggagtgcct tctcgtcgtc ggggacgtcg tcacctggaa cgagctcact
     6241 catcgggact cctatcgtgg acgcagattc ttggcacggt gtgccaacaa tctgacgtgt
     6301 gccaccgaca ctccgctcac ttcgactcgc gtgatgtctg ccatgcggct cggagcggta
     6361 ccgagcgcac aggacgaggg cggatcatgg ggtcgtgttg atcgacacga ctttcgacgt
     6421 caggaccgac gccgggggca aggacccgga cacgtggagt ccgacgctgt gtcgatatca
     6481 ccagttcctg tggagcaagc cgctaccgag cggagtctgc ttcgaactca cgccgaccag
     6541 ccgtccgccg tactacttcc gccacacatc gcaggttgga gacttcgccc tgtcgagcga
     6601 cgcgttcatc ccggcctaca cgcgctacgg cgtcccgaag tcggtgatcg aacagttgcc
     6661 gctcggcgag cacgtccact tcaacacgat cgggtacacc atcggcgggc tcatcctctg
     6721 gccggcaaac cggatcgacg gcaagtggac catcaaccag gcgcgtggat gcctccggag
     6781 gacgatcgga gaccggatgg acccgacgct cgagtgcgtc cggcgccact acagcggaga
     6841 gaagagcccg ctaggcgacg tactggcgcg gtacgccgac ttcttcgccg ccttcgagga
     6901 cttccgaggc tacgtcgact tctggctgct cagggacctg gtgaccgatg accactcggc
     6961 ggtccgcttc ttcacgccgt tcgacgactt ccggacgccg gcgatcccac ccgacctcga
     7021 cagctacgtc gcctatcgcc ggtgcagcat cgagttcctc gaggcccgca acgcccggat
     7081 cgccagggcg acgagggact cgcgaatgtg accggcccgg tgagggtcgc cggacgtcat
     7141 gcgggcggag caagcggatg ctctgaccgg atgacgtccc ggggccggcc ggggcgaggg
     7201 ccggccgtaa tctggacggc gtgcccagac tgctcgcccc gaggctgtgg ggtgcgcact
     7261 tgctggcgct ggtgctggtg ggggcggcgt tcgggttggg gctgtggcag tacggcgcgt
     7321 ggcaggacca ccgcgacgcg gaggcggtcg acctgaccca tgcgaagccc gagccgctcg
     7381 acgacgtgat cggtcccgac gacccgttcc cgggcgacaa gatcggccag ccggtgacgg
     7441 tcgacggcac ctgggtgccc gacggcacgg tctacgtctc c
//
LOCUS       DATBFZ010000152         1771 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_429136, whole genome shotgun sequence.
ACCESSION   DATBFZ010000152 DATBFZ010000000
VERSION     DATBFZ010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 1771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1771
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_429136"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..157)
                     /locus_tag="VH419_04630"
     CDS             complement(<1..157)
                     /locus_tag="VH419_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007078246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HEY7042935.1"
                     /translation="MTSPSGHVRVRFCPSPTGTPHVGLVRTALFNWAYARHTGGAFVF
                     RIEDTDAA"
     gene            complement(167..967)
                     /locus_tag="VH419_04635"
     CDS             complement(167..967)
                     /locus_tag="VH419_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009074058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEY7042936.1"
                     /translation="MRLGRIASPDGVAFVSVEGDGADAVCKEIAEHPFGNPNFTGRSW
                     PLADVRLLAPILASKVICMGKNYEAHAAEMGGVAPEDPVIFLKPNTAIIGPNVPIQLP
                     ADANPVHHEGELAIVIGRPCKDVPAARAAENILGYTIANDISARDQQAKDGQWMRAKG
                     HDTFCPVGPWIVNDLDPSDLEIRTEVTSGIAGAAGSEVRQLSRTSLMIHDIGAIVEWV
                     SAVMTLLPGDLILTGTPEGVGPIEDGDTVSVTVEGIGTLTNPVVRKQK"
     gene            complement(1028..1735)
                     /locus_tag="VH419_04640"
     CDS             complement(1028..1735)
                     /locus_tag="VH419_04640"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEY7042937.1"
                     /translation="MATAHSVVDSVIAMHHMDLPLSPKWAIVGQSQGGGAAVNSARWA
                     TEFSRGTGLDYRGVVATGTPFNVESIVKQAGPDMALPPNLGPAANSYTGYILAGVREA
                     RPELDIDSVLTPAGLDAVDKAETLCKPQLDQELTGMTPTGYFRAPLSTLPGVSEALDA
                     YLGTPTSGYDRPIFLGVGLLDRDVPPNMSLQLDEQLRANGQNVTLKVYPGEDHSGTVL
                     ASVPDSTPFLAAVFAGD"
BASE COUNT          276 a          575 c          611 g          309 t
ORIGIN      
        1 gagccgcatc ggtgtcctcg atccggaaca cgaacgcccc gccggtatgt cgcgcgtagg
       61 cccagttgaa cagcgccgtg cgaaccagcc cgacgtgcgg ggtcccggtc ggcgacgggc
      121 agaaccgcac cctgacatga ccagacggag acgtcatcac ttacctctac ttctgcttgc
      181 gtacaacggg attggtaagg gtgccgatgc cctcgacggt gacgctcacg gtgtccccgt
      241 cctcgatggg gccgacgccc tcgggagtac cggtgaggat caggtcaccc ggcagcagcg
      301 tcatcaccgc cgagacccac tcgacgatgg caccgatgtc atggatcatc agtgaggtcc
      361 ggctgagctg gcgcacttcg ctgccggcgg cgccggcgat tccactggtc acctcggtgc
      421 ggatctccag gtccgagggg tcgaggtcgt tgacgatcca cgggcccaca gggcagaagg
      481 tgtcgtggcc cttggcccgc atccactgac cgtccttggc ctgttgatca cgcgccgaga
      541 tgtcgttggc gatggtgtag cccaggatgt tctcggcagc acgcgcggcc ggcacgtcct
      601 tgcagggccg cccgatgacg atggccagct caccctcgtg gtgaaccgga ttggcgtcgg
      661 ccggtagctg gatcggcacg ttggggccga tgatcgcggt gttgggcttg aggaagatca
      721 ccgggtcctc gggcgctacc ccgcccatct ccgcggcgtg ggcctcgtag ttcttgccca
      781 tgcagatcac cttgctggcc aggatggggg ccaggagacg gacgtcggcc agcggccagc
      841 tccgtccggt gaagttggga ttgccgaacg gatgctcggc gatctctttg cagacggcat
      901 cggcgccgtc gccttcgaca ctgacgaaag cgacgccgtc gggactggcg attcgaccta
      961 agcgcatttt gaccagggta gtgcgcagcg tcttacgccc cgtgctgacg cctcctgtca
     1021 gtttccgtca gtcgcccgcg aacaccgcgg cgaggaacgg cgtggagtcc ggaaccgaag
     1081 ccagcacggt gcccgagtga tcttcgccgg gatagacctt gagggtgacg ttctgtccgt
     1141 ttgcccgcag ctgctcgtcg agctgcagcg acatgttcgg cgggacatcg cggtccagga
     1201 gtccgacgcc gaggaagatc ggccggtcgt aaccgctggt cggcgtgccc aggtaggcgt
     1261 cgagagcttc cgagacgccg ggcagggtgg acagcggcgc acggaagtaa ccggtcggcg
     1321 tcattccggt gagctcctgg tcgagctgcg gcttgcagag cgtctcggcc ttgtcgaccg
     1381 catccagccc ggccggggtg agcacgctgt cgatgtccag ttcgggccgc gcttcccgca
     1441 caccggcgag gatgtagccg gtgtagctgt tggccgcggg acccagattc ggcggcagcg
     1501 ccatgtccgg cccggcctgt ttgacgatgc tctcgacatt gaacggcgtg ccggtggcca
     1561 ccacaccgcg gtagtccagg ccggtccccc ggctgaactc ggtggcccag cgcgcgctgt
     1621 tgaccgcagc cccgccgccc tgggactgac cgacgatggc ccatttcggc gacaacggga
     1681 ggtccatgtg gtgcatcgcg atcaccgagt cgaccacgct gtgcgcggtg gccacgctgt
     1741 tgaggtagct catcaggccc ggggtaccca g
//
LOCUS       DATBFZ010000153         2246 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_2068185, whole genome shotgun sequence.
ACCESSION   DATBFZ010000153 DATBFZ010000000
VERSION     DATBFZ010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2068185"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..2028)
                     /locus_tag="VH419_04645"
     CDS             complement(139..2028)
                     /locus_tag="VH419_04645"
                     /inference="COORDINATES: protein motif:HMM:NF015140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HEY7042938.1"
                     /translation="MALVSLVSLVAAPAAHVGVAAATPPSTGSVIALGTTGHFGNAGT
                     FPDAFSDPNPELLPNVGQVVAVAGDNSTGVALLPDGTVWSWGSNHYGQLGDGTTTDSA
                     TPVEAIGVSNAVAISSDGVHTLALTSGGSVWAWGANAAGQLGDGTTTDRSTPVPVSGL
                     SNVVAISSDNNAYNTGGVGLALLADGTVWSWGSNDYGVLGTGSGAEYSAVPAQIPGLS
                     NVVSVSTSGLHSLAATSDGSVWAWGAGFYDELGDGGGGPFHFALTPIQVPGVGSITSV
                     SAGDVDSTALASDGTVWFWGAYFANPKNFSGFPPGQVRGLSDVASVTAGRDAVFAVTS
                     DGSLWDWGTNTVGQLGDGNLTTQLAPSEVTTIAGVTSVSSTGALLVGSLVGCTGVCAS
                     IGDVMGWAGGAMAFPITLNKPAATDTTITVWINNDPVSSQVGPCCLEFGLQYSTFKMV
                     TIKAGHIEGFVTFSTKDLSTYFPAVPPSQVPFAATLISSSATSIARAGGVGLENDSAD
                     LPGYLHIGAARIVEPDSGKVTVRIPVLLGAADPVNKVTVQYRTADGTATAGNDYVAKP
                     KITNPPATLTIPRMVRRASVAVTVLGHMTPEATKTFSINFTNPMFATIPYGTVAPVTI
                     LDNGD"
BASE COUNT          377 a          762 c          763 g          344 t
ORIGIN      
        1 gctgtcagac gcttctcggc cgctccggcc tcgggagtcg cgctgcccat cgtctcgcct
       61 ccgtcgtcca gggcacggtc ggctcccggc gacagcccga ggatagaccg aggtcgaagc
      121 gagccgccca gcggccgatc agtccccgtt gtcgaggatc gtgacgggcg cgaccgtgcc
      181 gtaggggatc gtcgcgaaca tcgggttggt gaagttgatc gagaaggtct tggtggcttc
      241 gggggtcatg tgaccgagca cggtcacggc cacagacgcc cgtcgcacca tgcgcgggat
      301 ggtcaaggtg gcgggtgggt tcgtgatctt gggcttcgcg acgtagtcgt tgcccgccgt
      361 cgccgtgccg tcggcggtcc ggtattggac agtgaccttg ttcaccgggt cggccgcgcc
      421 cagcagcacc gggatcctga cggtgacctt gccactgtcg ggctcgacga tgcgcgctgc
      481 accgatgtgg agatatccgg gcaggtccgc cgagtcgttc tcgaggccga cgccgccggc
      541 ccgggcaatc gacgtggccg acgagctgat cagggtggcc gcgaacggga cttgggacgg
      601 gggcaccgcc gggaagtacg tcgagaggtc cttggtcgag aaggtgacga aaccttcgat
      661 gtggccggct ttgatcgtga ccatcttgaa ggtgctgtat tggaggccga actcgaggca
      721 gcaagggccg acctgcgagg acaccgggtc gttgttgatc cacaccgtga tcgtggtgtc
      781 ggttgccgcg ggcttgttca gcgtgatcgg aaaggccatc gccccgcctg cccagcccat
      841 cacgtcgccg atggacgcgc agacccctgt gcatccgacc aggcttccga cgaggagcgc
      901 gccggtggag ctcaccgagg tgacgccggc gatggtcgtg acctccgacg gcgcgagctg
      961 tgtcgtcaag ttgccgtcac cgagctgacc gaccgtgttg gtcccccagt cccacaggct
     1021 cccgtcggac gtcaccgcga acaccgcgtc gcgccccgcc gtcacggatg ccacgtcgga
     1081 gaggccccgc acctggccgg gtgggaaccc ggagaagttc ttcgggttcg cgaagtacgc
     1141 accccagaac cacacggtgc cgtcggacgc cagcgccgtg ctgtccacgt cgcccgcact
     1201 gaccgacgtg atcgagccga ccccaggcac ttggatcgga gtcagcgcga agtggaatgg
     1261 gccacctccc ccgtcgccga gctcgtcgta gaaacccgcg ccccacgccc agaccgatcc
     1321 atcgctggtc gccgccagac tatggaggcc gctcgtcgac acggagacca cgttcgacag
     1381 gccgggaatc tgcgcaggca ccgcgctgta ctccgcgccc gaccccgtac cgagcacgcc
     1441 gtagtcgttg gagccccacg accacacggt cccatccgcg agcagtgcca ggcccactcc
     1501 accggtgttg tatgcgttgt tgtcgctgct gatagcgacc acattggaga gcccactcac
     1561 cgggacaggc gtggaacggt ccgtcgtcgt gccgtcaccg agctggccgg ccgcgttcgc
     1621 gccccacgcc cacacggacc cgcccgacgt gagcgccaac gtgtgcacac cgtccgagga
     1681 gatcgcgacc gcattcgaca ccccgatcgc ctcgaccggc gttgcgctgt ccgtcgtggt
     1741 cccgtcaccg agctgcccgt aatggttgga accccacgac cacacggttc cgtcgggaag
     1801 gagtgcgacg cccgtcgaat tgtcaccggc aacggcgacc acctgtccga cattcggaag
     1861 cagctcggga ttcggatcag agaacgcatc cgggaaagtc ccggcgttgc cgaagtgccc
     1921 cgtagtcccg agcgcgatga cggagccggt ggaaggtggg gttgccgccg cgacaccaac
     1981 atgcgcagcg ggtgcggcga ccaggctcac gagagaaacg agagccatcc agacgatccg
     2041 ccgcatgcca ccccccgtga cacccgtgat ccagggccta cgggcgggaa cgtatcagcg
     2101 gcgggtcaac gcggaaaggg gtaggcgtca gaccgcggcg tagaccgcgg gtcagacggc
     2161 gtggcggcgg ccgacgatgt cgcggcggcc caggtggttc cagcggcagt cggtgcacac
     2221 ctcgaccacg taccgatcga actcgt
//
LOCUS       DATBFZ010000154         2883 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_1086009, whole genome shotgun sequence.
ACCESSION   DATBFZ010000154 DATBFZ010000000
VERSION     DATBFZ010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2883
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1086009"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="VH419_04650"
     CDS             complement(<1..432)
                     /locus_tag="VH419_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase-like domain-containing protein"
                     /protein_id="HEY7042939.1"
                     /translation="MPLAIEDYALLGDTGTCALVGRDGSVDWLCLPRFDSQACFAALL
                     GEPRHGRWLIGPKARATTSRRYVGNTFVLETTHETETGAVRVTDVMPLGDGRADLVRR
                     VEGLRGTVEMYHEWVVRFGYGKIRPWVTRRKDEEGREVVSAI"
     gene            522..728
                     /locus_tag="VH419_04655"
     CDS             522..728
                     /locus_tag="VH419_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008361292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5703 family protein"
                     /protein_id="HEY7042940.1"
                     /translation="MEYEFQRFSLPRTASRSAVRRLLIDHAEYGGWELARLRLFPDGT
                     RKVELRRKIIRVRSTVTPDVRPMI"
     gene            complement(736..2040)
                     /locus_tag="VH419_04660"
     CDS             complement(736..2040)
                     /locus_tag="VH419_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008361295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEY7042941.1"
                     /translation="MTKAYTPEAEVVQICRELIRMDTTNYGDDSGPGERSAAEYVAGL
                     LDEVGIESQLFEPTKGRTSVVAHWGDPNATDRLLLHGHLDVVPANRADWKVDPFSAEE
                     VDGYIWGRGAVDMKDFDATILSVVRARERAGERPNRAITLAFTADEEAGSRQGAHPLA
                     DQHPDWFDGVTEGIGEVGGFSATVAGKRLYLVETAEKGLAWLRLRAQGTAGHGSMRNT
                     DNAVTAVSEAVAAIGRHEWPVRLTPSMERLLEEVATLTGHENVEDNVDALIEHFGPAA
                     RMLAAVVRNTANPTMLDSGYKHNVVPGEATAAIDGRFLPGYEEEFFSTLRSLLPDNVS
                     YEVLVHDTALETPFDGPITDAMTAALLAEDPEATVLPFLMSGGTDAKAWSKLGIRCFG
                     FSPLRLPADLDFTALFHGVDERVPVDALEFSARVLDGLLERA"
     gene            2139..2227
                     /locus_tag="VH419_04665"
     tRNA            2139..2227
                     /locus_tag="VH419_04665"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2173..2175,aa:Leu,seq:gag)
     gene            2490..>2883
                     /locus_tag="VH419_04670"
     CDS             2490..>2883
                     /locus_tag="VH419_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042942.1"
                     /translation="MPAHHDDAHVHALGTEAALTSLVNASLANIPGADMASVTIRSQD
                     QHLSTAAASDDLAYRLDEIQYELREGPCYDAVTISQFALDNDLRHGRYPRYGPRAADL
                     GLGSQLATQVAHNGTQAGLNVYARRVHAF"
BASE COUNT          446 a         1044 c          932 g          461 t
ORIGIN      
        1 gatcgccgag acgacctcgc ggccctcctc gtccttgcgt cgggtcaccc atggccggat
       61 cttgccgtag ccgaaccgga ccacccactc gtggtacatc tcgacggttc cgcgcaagcc
      121 ctcgacccga cggaccaggt cggcgcgtcc gtcaccgagc ggcatcacgt cggtcacccg
      181 gaccgcgccg gtctcggtct cgtgcgtggt ctccagcacg aacgtgttcc cgacgtagcg
      241 gcggctggtg gtggcccgcg ccttcggacc gatcaaccaa cggccgtgcc ggggctcgcc
      301 cagcaacgcc gcgaagcacg cctgcgagtc gaagcgaggc aggcacaacc agtccaccga
      361 cccgtctctg ccgaccaggg cacacgtgcc cgtgtcaccc agcagggcat agtcctcgat
      421 cgccaacggc atgcgcccga ggctagccgg gggtcgggcg agcggcagtg gtctgcgcgt
      481 ctgggccgca gacacttggc cggcgtgtca ccatggtcgc gatggagtac gagttccagc
      541 gattctcgct cccgcggacg gcctcgcggt ccgcggtacg ccgactgctc atcgaccatg
      601 cggagtacgg cggctgggaa ctcgcccgac tgcggctctt ccccgacggc acccggaagg
      661 tcgagctccg ccgcaagatc atccgggtcc gctcgaccgt cacccccgac gtccgcccga
      721 tgatctgacg ctcgctcagg cgcgctcgag caagccgtcg agcacccgcg cggagaactc
      781 caaggcgtcg accggcaccc gctcgtcgac cccgtggaac agcgcggtga agtccaggtc
      841 ggccggcagc cggaggggcg agaagccgaa gcagcggatc ccgagcttgc tccacgcctt
      901 cgcgtccgtg ccccccgaca tcaggaacgg aagcacggtt gcctccgggt cctcggccag
      961 cagcgcggct gtcatcgcgt cggtgatggg cccgtcgaac ggcgtctcga gggcagtgtc
     1021 gtggaccagg acttcgtacg agacgttgtc cgggagcaac gatcgcaggg tcgagaagaa
     1081 ctcctcctcg tagcccggca ggaaccgccc gtcgatcgcc gctgttgcct ctcccgggac
     1141 cacgttgtgc ttgtagccgg agtcgagcat cgtcgggttg gccgtgttgc ggacgacggc
     1201 cgcgagcatc cgggctgccg gtccgaagtg ctcgatcagc gcatcgacgt tgtcttcgac
     1261 gttctcgtgg ccggtcagcg tcgcgacttc ctccagcagc cgctccatcg agggcgtcag
     1321 ccggacgggc cactcgtgcc gaccgatggc cgcgaccgct tcggagacag ccgtgaccgc
     1381 gttgtcggtg ttccgcatcg acccgtgtcc ggccgtgccc tgggcccgga gccgcagcca
     1441 ggcgagtccc ttctccgcgg tctcgaccag gtagagccgc ttgccggcaa cggtcgcgga
     1501 gaaccctccg acctcgccga tcccctcggt cacgccgtcg aaccagtccg ggtgctggtc
     1561 ggccagcggg tgcgccccct gccggctgcc ggcttcctcg tcggcagtga aagccagcgt
     1621 gatcgcgcgg ttcgggcgtt ccccggcacg ctcgcgagcc cggacgaccg agaggatcgt
     1681 cgcgtcgaag tccttcatgt cgacggcgcc gcggccccag atgtagccgt cgacctcctc
     1741 ggccgagaac gggtcgacct tccagtccgc cctgttggcg ggaacgacat cgagatggcc
     1801 gtgcagcagc agccggtcgg tggcgttcgg gtcgccccag tgcgccacca ccgaggtgcg
     1861 tcccttggtc ggctcgaaca gctgtgactc gatccccacc tcgtcgagca gacccgcgac
     1921 gtactccgcc gcagaccgct cccccggacc ggagtcgtcg ccgtagttcg tggtgtccat
     1981 ccggatcagc tcgcggcaga tctggacgac ctcggcctcg ggcgtgtacg ccttcgtcat
     2041 accgccatct tgccgtgccg gattacgtcg caccgatcgg gctttgctaa ggttggtggc
     2101 cgctcgtcgg gccgcgttgc gcgccccgat gagcccttgt ccgggtggcg gaattggcag
     2161 acgcgctagc ttgaggtgct agtgcccgta ttagggcatg ggggttcaag tcccccctcg
     2221 gacaccagtg ttcttcggat gaaaccgctg gtcagacggc ctgcagctta gtcgcgagag
     2281 cgagacgacc cgtccatcac gccgaggatc ggcagccacg tcctgtcgag gcggtccttt
     2341 cgtatgcctt gttggccgag tagcgtgaag tgatcgatgc acgtgtgtcc aggacagggc
     2401 acgccacgtc agaaatcgag agcgtgtctc cgtcagccca gcgacagagg ttgggtggcg
     2461 gcctcgacgt tcagggaaga ccccgaatca tgcccgccca ccacgacgat gcccatgttc
     2521 atgccctggg caccgaagcc gccctcacca gcttggtcaa cgcatcactg gccaacatcc
     2581 ccggcgccga catggccagc gtcaccatcc gcagccagga ccagcatctg agcaccgcgg
     2641 ccgccagcga cgacctagcc taccggctcg acgaaatcca gtacgagcta cgcgagggcc
     2701 cctgctacga cgccgtcacc atcagccagt tcgccttgga caacgacctg cgccacggcc
     2761 gttacccccg ctacggtcca cgcgctgccg acctcggtct cggctcgcag ctggccaccc
     2821 aggtcgctca caacggcacc caggcggggc tgaacgtcta cgcgcggcgc gtccacgcgt
     2881 tcg
//
LOCUS       DATBFZ010000155         6991 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_2069463, whole genome shotgun sequence.
ACCESSION   DATBFZ010000155 DATBFZ010000000
VERSION     DATBFZ010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 6991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6991
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2069463"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /locus_tag="VH419_04675"
     CDS             complement(<1..654)
                     /locus_tag="VH419_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEY7042943.1"
                     /translation="MSADPTTPTRSHHPRCAVLGSPIAHSLSPAIHRAAYAELGLDWR
                     YDALEVVAEELTGFVAGLGDEWRGLSLTMPLKQPAVALCDSVEPLAGRLGAVNTMVRT
                     ALAWSGHNTDVGGSVDALRDAGVADVRTAVVVGSGATAGSVLAALAELGVARVTVLAR
                     SLDRAGWLVSLGRDLGPAVDLARLDVRPGGDVVRGDILASTVPIDAQAVYAAQLTALV
                     "
     gene            complement(644..1780)
                     /gene="mltG"
                     /locus_tag="VH419_04680"
     CDS             complement(644..1780)
                     /gene="mltG"
                     /locus_tag="VH419_04680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEY7042944.1"
                     /translation="MTDTDTDTSLGVGGLRSTRHRSKVRRTFGCFPILVAVAIIAALG
                     TLGYVKGVDFIKDQLSGPPDYSGDGQKPTVTVEVEKGDTATDIGHTLYDAGVVKSVEA
                     FTDAASADERSLGIQVGRYLLLSKMSARSALLVLIDPDSLIKGPTITIPEGLRAEETL
                     ASIAKQTHLTVRQLQTAYHDAAALDLPAYAGGNPEGFLFPSTYEVTSATTAAGLLHQM
                     VATFKQEAAAMHLEEKAKQLGHSPYEVVTVASLVQAESGTADMSKVASVVYNRLDAGM
                     PLELDSTLHYALGLRGDVVTTEEQRKLDSPYNTYKVAGLPPTPINSFGANALEAALNP
                     ADTDYLYFVTVNLATGETKFASSYQEHLRNVAQYRRYCETSDEC"
     gene            complement(1785..2303)
                     /gene="ruvX"
                     /locus_tag="VH419_04685"
     CDS             complement(1785..2303)
                     /gene="ruvX"
                     /locus_tag="VH419_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEY7042945.1"
                     /translation="MRRGVRLGIDVGDVRIGVARTDPDALIATPVETVSRGPGDLARL
                     VDLTRREQAIEVLVGLPVSLSGQSGPAADKAREFAARLQAALDAPSGPDEAGWTGGPA
                     EVPGTVSSVRVRLVDERLTTVSAERVLREQGKKGAKRRSVVDQAAAVVILQHAIDTER
                     STGRPPGQEVGR"
     gene            complement(2311..4986)
                     /gene="alaS"
                     /locus_tag="VH419_04690"
     CDS             complement(2311..4986)
                     /gene="alaS"
                     /locus_tag="VH419_04690"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012921960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEY7042946.1"
                     /translation="MDTAEIRRRFITHFEQRDHHVVPSASLLLDDPNLLFVNAGMVPF
                     VPYFLGHEPAPFPRAVSVQKCIRTLDIDEVGKTDRHGTFFQMNGNFSFGDYFKEGAIE
                     AAWDLVTKPRSDGGYGIDESVLHVSVYLDDDESVAIWKRVAGLHDDRIHRLGMADNYW
                     SMGIPGPCGPDSELFVDRGPAHGPDDLEKAQDRFMEFWNLVFMQSLRGEGTGKDSFEI
                     LGDLPKKNIDTGMGLERMAMLLQGVGSIYEIDEVNPVLERAAELAGKRYGADHDDDVR
                     LRVVADHVRSALMLIGDGVTPSNEARGYVLRRILRRVVRSMRLLGVEDHTLPELLPVS
                     LSKMRLSYPELDTEWPRISAVAYAEEEAFRQTLKAGTTMFDLAVTETKQQGGAALSGA
                     KAFQLHDTYGFPIDLTLEMAAEQGLAVDEVGFRDLMAEQRRRAKADAMAKKGQHTDTS
                     AYRQIADGLGRAVTFTGYDEVVSEATVVGLVTDDGVVQQVGEGDTFELVLDRTPFYAE
                     GGGQLADQGVITLDNGARIQVLDVQAPTGGVIVHKASVVEGEVTPGVAAHAVVDVERR
                     KSISRAHTATHMVHKAMREALGETATQAGSENAPGRFRFDFSATGAVPPSVLHDVEAK
                     VNDLVLTDLAVHADVMTQEQARSIGAMALFGEKYGDEVRVVSVGDWARELCGGTHAGR
                     SGQLGVIKLLGESSIGSGVRRVEALVGADAYRFLAREHVLVAQLTEALKVRREELPER
                     VNDIVERLRSAEKEIERVRVAALLSRAGELADAARDVFGVRYAGHFADGANGADVRRL
                     ALDVRGRLGGDRPAVVAVIGASQGKPSVVVALNDTARDRGLSAGQLVKVAATTLGGSG
                     GGKDDVAQGGGSNADAAPEALQAVERAIGERVAGG"
     gene            complement(5060..5359)
                     /locus_tag="VH419_04695"
     CDS             complement(5060..5359)
                     /locus_tag="VH419_04695"
                     /inference="COORDINATES: protein motif:HMM:NF040339.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6167 family protein"
                     /protein_id="HEY7042947.1"
                     /translation="MFWFAAGAASGVYTLVKVRRTAQNLTPDGVAARVAAWGVGARLF
                     RDEVALGMEERERELRAQLRVPAPARRELEQGRELRTERATGAAAAATASLRANA"
     gene            complement(5365..5985)
                     /locus_tag="VH419_04700"
     CDS             complement(5365..5985)
                     /locus_tag="VH419_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042948.1"
                     /translation="MTSNVAAVLAVAAVVLVVVLGWLVLRLRGQLRELQTTIASLDRR
                     QAELVDRLDESAREQHDTWQAVELALLGQESASRTDGVATGPTGSTGSTGSNGSNGST
                     GSTGSTGGPPDEITVITDIDNRDRDRSADLSTARVASVTLGGPLIKVAAFSHGVRHAL
                     DEEQRLRIRYAVRKELRHQRKMRRRRRTERAPSKGRATVDERGATR"
     gene            complement(6047..>6991)
                     /locus_tag="VH419_04705"
     CDS             complement(6047..>6991)
                     /locus_tag="VH419_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEY7042949.1"
                     /translation="IAATTENPFFSVISPLLSRSLLLTLEPLKDDAVRELVDRALVDE
                     RGLAGTVTIVPEALDQLVRLAGGDARRALTYLEAAALSAGVKGEHSISMQTLESAVDK
                     AAVRYDRQGDQHYDVISAFIKSIRGSDVDAALHYLARMVTAGEDPRFIARRLVILASE
                     DIGLADPTALPTAVAAAQAVQLIGLPEARINLAQAVIALSLAPKSNAVITAVDAALAD
                     VAAGRIGTVPSHLRDAHYAGARKLEHGKGYQYAHDDPRGIAPQRHAPTELLDATYYRP
                     SLHGTEAEYAARLDRIKAVLNEPEYSADDGETGEPGPP"
BASE COUNT         1020 a         2485 c         2357 g         1129 t
ORIGIN      
        1 aaccagcgcg gtcagctgtg ccgcgtacac cgcctgagcg tcgatgggga ccgtggaggc
       61 aaggatgtca ccccgcacga cgtccccgcc cggacgaaca tccagcctgg cgagatcgac
      121 cgccggcccg aggtcccgac cgagcgatac gagccagccg gcccggtcga gcgaccgcgc
      181 cagcaccgtc actcgcgcga caccgagctc ggcgagcgca gccagtacgg aaccggccgt
      241 cgcgcccgat cccacgacga ccgcggttcg gacatcggcc actccggcgt cccgcagcgc
      301 gtcgaccgat ccgccgacgt cggtgttgtg gcccgaccat gcgagggcag tgcggaccat
      361 cgtgttcacg gcacccagcc gtccggccag cggttcgacc gagtcgcaca gggcgaccgc
      421 cggctgcttg agcggcatcg tcagcgagag cccgcgccat tcgtcaccga gtcccgccac
      481 gaaacccgtc agctcctccg cgaccacctc gagggcgtcg tagcgccagt cgagacccag
      541 ctcggcgtac gccgcccgat gaatcgcggg cgacagcgag tgcgcgatcg gagagcccag
      601 gacggcgcag cgggggtggt gcgaccgtgt cggtgtcgtc gggtcagcac tcatccgacg
      661 tctcgcagta ccgcctgtac tgggcgacgt tgcgcagatg ctcctggtag gacgaggcga
      721 acttcgtctc accggtcgcg aggttgaccg tgacgaagta caggtagtcg gtgtcggccg
      781 ggttgagcgc cgcctcgagc gcgttggcac cgaacgagtt gatcggggtc ggcggcagac
      841 cggcgacctt gtacgtgttg tacggcgagt cgagcttgcg ttgctcctcc gtcgtgacga
      901 cgtcgccacg cagccccaac gcgtagtgca gcgtcgagtc cagctcgagc ggcatgccgg
      961 cgtccaaccg gttgtagacg acgctggcca ccttggacat gtcagcggtg cccgactcgg
     1021 cctgcaccag gctcgcgacc gtcaccacct cgtacggcga gtggcccagc tgcttggcct
     1081 tctcctccag atgcatggcg gcggcctcct gcttgaacgt cgccaccatc tggtgcagca
     1141 gccctgcggc cgtcgtcgcc gatgtcacct cgtaggtcga cgggaagagg aacccttccg
     1201 ggttgccgcc cgcgtacgca ggcaggtcga gtgcggccgc gtcgtggtac gccgtctgga
     1261 gctgcctgac cgtgaggtgg gtctgcttgg cgatcgacgc cagcgtctcc tcggctcgca
     1321 ggccttcggg gatcgtgatc gtcgggcctt tgatcagcga gtcggggtcg atgagcacga
     1381 gcagcgcgct gcgcgccgac atcttcgaca gcagcaggta tctgccgacc tggataccca
     1441 acgatcgttc gtcggcgctg gcggcgtcgg tgaaggcctc gaccgacttg acgacgccgg
     1501 cgtcgtacaa cgtatggccg atgtcggtcg cggtgtcacc cttttcgacc tcgacggtga
     1561 ccgtcggctt ctgtccgtcg ccggagtagt ccggcggacc ggacagctgg tccttgatga
     1621 agtcgacacc tttcacgtag ccgagcgtcc cgagcgcggc gatgatggcg actgcgacca
     1681 ggatggggaa gcaaccgaac gtgcggcgca ccttggagcg gtgacgcgta ctccgcaacc
     1741 cgccgacccc cagagacgtg tccgtgtcgg tgtcggtcat cggctcagcg gcctacttcc
     1801 tgcccaggtg gccgccccgt cgaccgttcg gtgtcgatgg cgtgctgcag gatgacgacg
     1861 gcggccgctt gatcgaccac agaccgccgc ttggcaccct tcttgccttg ctcacgcagc
     1921 acacgctccg cgctcaccgt ggtcagcctc tcgtcgacca ggcgcacgcg tacggacgaa
     1981 accgtaccag gcacctcggc gggtccgccc gtccagcccg cctcgtctgg accgctcggc
     2041 gcgtccaggg ccgcctgcag ccgggccgcg aactcccggg ctttgtcggc tgcaggtccg
     2101 gactggcccg acagggagac cggcagtccc acgaggactt cgatcgcctg ctcccggcgc
     2161 gtcaggtcca ccagtcgggc caggtcgccg gggccacggg acaccgtctc gaccggtgtc
     2221 gcgatcagcg cgtccggatc ggtgcgcgcc acgccgatcc ggacgtcacc gacatcgatc
     2281 cccagccgga ccccacgacg gatcgcgggt tcagccaccg gccacccgtt cgccgatcgc
     2341 gcgctcgacc gcctgcagtg cctccggcgc cgcgtcggcg ttcgacccac caccttgggc
     2401 gacgtcgtcc ttgccgcctc cgctgccacc gagggtggtc gctgcgacct tcaccagctg
     2461 gcccgccgac aggcctcggt cgcgggcggt gtcgttgagc gcgaccacga ccgagggctt
     2521 gccctgactc gcaccgatca ccgccaccac ggcaggccgg tcgccgccga gccggccgcg
     2581 gacatccagc gcgagccgtc gtacgtccgc gccgttggcc ccgtccgcga agtgtccggc
     2641 gtagcggacc ccgaagacgt cgcgagcggc gtccgccagc tcgccggcac gagacagcag
     2701 cgccgccacc cgcacccgct cgatctcctt ctctgccgag cggagccgtt cgacgatgtc
     2761 gttgacgcgt tccgggagct cctcgcggcg caccttgagc gcctcggtga gctgcgcaac
     2821 caagacgtgc tcgcgcgcga ggaaccgata ggcatcggca cccacgagcg cctcgacgcg
     2881 gcggacgccg gagccgatcg acgactcgcc gagcagcttg atcacgccga gctgcccgga
     2941 ccggcccgcg tgcgtgcctc cgcacagttc ccgcgcccag tcaccgacgg agacgacacg
     3001 cacctcgtcg ccgtacttct ccccgaacag cgccatcgcg ccgatcgacc gcgcctgctc
     3061 ctgcgtcatc acgtcggcgt gcacggccag gtcggtcagg acgaggtcgt tgaccttcgc
     3121 ctccacgtcg tggaggacgg acgggggcac ggcgccggtg gcggagaagt cgaagcggaa
     3181 ccggccgggc gcgttctcgc tgccggcctg ggtggcggtc tcccccaacg cctcccgcat
     3241 cgccttgtga accatgtggg tggcggtgtg cgcccggctg atcgacttgc gccgctcgac
     3301 gtccacgacc gcgtgggctg cgacacccgg tgtgacctcg ccctccacga ccgacgcctt
     3361 gtgcacgatg acgcctccgg tcggggcctg cacgtcgagc acttgaatgc gggcaccgtt
     3421 gtccagcgtg atgacgccct ggtccgcgag ctggccgccg ccctcggcgt agaacggcgt
     3481 acggtccagc acgagctcga acgtgtcgcc ctcgccgacc tgctgcacga ctccgtcgtc
     3541 ggtgacgaga ccgacgacgg tcgcttcgga cacgacctcg tcgtacccgg tgaacgtcac
     3601 cgcacggccc aacccgtccg cgatctgccg gtacgccgag gtgtccgtgt gctgaccctt
     3661 cttcgccatc gcgtcggcct tcgcgcgccg ccgctgctca gccatcaggt cgcggaagcc
     3721 gacctcgtcg acggcgagtc cctgctcggc cgccatctcg agcgtgaggt cgatcgggaa
     3781 gccgtacgtg tcgtgcagct ggaacgcctt ggcgccggag agcgcggcgc cgccctgctg
     3841 cttggtctcg gtgacggcca ggtcgaacat cgtcgtcccc gccttcagcg tctgccggaa
     3901 ggcctcctcc tcggcgtacg cgaccgccga gatccgcggc cactcggtgt ccagctcggg
     3961 gtaggacaac cgcatcttcg acagtgacac cggcagcagc tcggggagcg tgtggtcctc
     4021 gacgccgagc aggcgcatcg accgcacgac ccggcgcagg atgcggcgca agacgtagcc
     4081 acgggcttcg ttggacggtg tcacgccgtc cccgatcaac atgagcgcgc ttcggacgtg
     4141 gtccgcgacg acgcgcaacc ggacgtcgtc gtcgtggtcg gcgccgtagc gcttgcccgc
     4201 cagctcggcc gctcgctcga gcaccgggtt gacctcgtcg atctcgtaga tgctgccgac
     4261 accctggagc agcatcgcca tccgctccag acccataccg gtgtcgatgt tcttcttcgg
     4321 gaggtcaccg aggatctcga agctgtcctt gccggtgcct tcgccgcgca gactctgcat
     4381 gaacacgagg ttccagaact ccatgaaccg gtcttgtgcc ttctccagat cgtccggacc
     4441 gtgcgccggc ccgcggtcga cgaacagctc cgagtccggc ccgcaggggc caggtatccc
     4501 catcgaccag tagttgtcgg ccatgccgag gcggtggata cggtcgtcgt ggagtccggc
     4561 aacgcgtttc cagatggcca ccgactcgtc gtcgtcgagg tacaccgaca cgtgcagcac
     4621 ggattcgtcg atgccataac cgccgtcgga ccgcggcttc gtgaccaggt cccaggccgc
     4681 ctcgatggcg ccttccttga agtagtcgcc gaacgagaag ttgccgttca tctggaagaa
     4741 cgtgccgtgc cggtcggtct tgcctacttc gtcgatgtcg agtgtgcgga tacatttctg
     4801 cacgctgacc gcccgaggga acggtgcggg ttcatgaccg aggaagtacg gcacgaacgg
     4861 gaccatgccg gcgttgacga acagcaggtt cgggtcgtcg agcagcagag acgcgctcgg
     4921 gacgacgtga tggtcgcgtt gctcgaagtg cgtgatgaat cgtcgtcgga tctcggcggt
     4981 gtccatggtc aaggctttct caggtgagcc acggctgtgt acgtgatcgg cgcgtcacag
     5041 cgacggcagg gtcggatggt caggcgttcg cgcgaagcga ggctgtggcg gcggctgccg
     5101 ccccggtagc tcgctccgtg cggagctccc gaccctgctc gagctcgcgt cgcgcaggtg
     5161 cgggcacgcg gagctgcgct cgcagctcgc gttcgcgctc ctccatcccc agcgcgacct
     5221 cgtccctgaa cagtcgggcc ccgacacccc acgctgcgac ccgcgcagca acaccgtcag
     5281 gggtgaggtt ctgcgccgta cgccggacct tgacgagcgt gtagacaccc gaggcagcgc
     5341 ccgcggcaaa ccagaacatg cggctcaacg ggttgctcct ctctcgtcga ccgtggccct
     5401 ccccttcgag ggtgctcgct cggtgcggcg acgtcgacgc attttccgct ggtggcgcag
     5461 ctccttgcgc acggcgtacc ggatccgcag ccgttgctcc tcgtccagcg cgtgccgcac
     5521 gccgtgggag aaggcagcca ccttgatgag cggaccaccc agcgtgaccg atgcgacgcg
     5581 cgctgtgctc agatctgccg aacggtcgcg gtctcggttg tcgatgtcgg tgatcaccgt
     5641 gatctcgtcg ggcggtccgc cggtcgagcc ggtcgagccg gtcgagccgt tcgagccgtt
     5701 cgagccggtc gagccggtcg agccggtcgg gcccgtcgcg accccgtcgg tcctggaggc
     5761 ggactcctgt ccgagcaggg cgagctcgac ggcctgccag gtgtcgtgct gctcccgcgc
     5821 cgactcgtcc agtcggtcga cgagctcagc ctgccgccgg tcgagcgacg cgatcgtcgt
     5881 ctgcagctcc cgcagctgcc cgcgaagacg gagtacgagc cagccgagga ccaccacgag
     5941 cacgaccgcc gccacggcca gcacggctgc aacgttgctc gtcaccgcgt ccgtcctttg
     6001 ctcgtagcgt cgatggttcg gccaagcggt aaccctaccg cgcaggtcag ggaggccccg
     6061 gctctcccgt ttccccgtcg tcagcggagt attcgggttc gttcaagacg gctttgatgc
     6121 ggtcgagccg agcggcgtac tccgcctccg tcccgtgcag gctcggtcgg tagtaggtcg
     6181 cgtcgaggag ctcggtcggc gcatggcgct gcggcgcgat cccgcgcggg tcgtcgtgcg
     6241 catactggta acccttgccg tgctcgagct tccgggcgcc ggcgtagtgc gcgtctctga
     6301 gatgcgacgg gaccgttccg atccttccgg cggcgacgtc ggcgagcgcg gcgtcgacgg
     6361 ctgtgatgac ggcgttcgac ttcggcgcga gcgacagcgc gatgaccgcc tgggcgaggt
     6421 tgatgcgtgc ctcgggcagt ccgatcagct gcacggcctg cgcggcagcc acggccgtcg
     6481 gcagcgcggt gggatcggcg agcccgatgt cctcactggc gaggatcacc agccgccgag
     6541 cgatgaagcg cggatcctca ccggcggtga ccatccgggc gagatagtgc agtgcggcgt
     6601 cgacatcgct cccacggatc gacttgatga acgcgctgat gacgtcgtag tgctggtcgc
     6661 cctgccggtc gtaccgcacg gccgccttgt cgactgccga ctccagcgtt tgcatcgaga
     6721 tgctgtgctc acccttgacg cccgcactga gcgccgccgc ctccaggtag gtgagtgcac
     6781 gccgggcgtc tccgcctgcc agccgcacga gctggtcgag cgcctcgggc acgatcgtca
     6841 ccgtgccggc caggccacgc tcgtcgacga gcgcccggtc gaccagctct cgcacggcat
     6901 cgtccttcag cggctcgagc gtgagcagca gcgagcgtga cagcagcggc gagatgacgg
     6961 agaagaaggg gttctcggtg gtggcggcga t
//
LOCUS       DATBFZ010000156         2327 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_1412149, whole genome shotgun sequence.
ACCESSION   DATBFZ010000156 DATBFZ010000000
VERSION     DATBFZ010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1412149"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="VH419_04710"
     CDS             <1..531
                     /locus_tag="VH419_04710"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY7042950.1"
                     /translation="LLDMSRLDAGALQPRLEWVSLSDVVADVLDRMTPMLAGHQVLVL
                     VPDTLPATPMDFVLISQVLTNLLDNAVRYAPRGAAITISGEIVRDQLRVTVFNEGTHL
                     PDQDVERVFDKFYRVSDVPGGTGLGLAIARGNVEAHHGHMWAENVGQRGVSFIFTLPS
                     PPPDVVTQELAVAKSA"
     gene            540..1208
                     /locus_tag="VH419_04715"
     CDS             540..1208
                     /locus_tag="VH419_04715"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY7042951.1"
                     /translation="MTRILLVDDESDVRNTVRRGLQIQGYEVETAVDGEEAVLKTRGW
                     QPDVVLMDLAMPKMGGISAIEEIRHWSEVPIIVLSVMGEEREKVRALDSGADDYLTKP
                     FGMQELSARIRATLRRRAPAPEGRVYTFGTVGVDTTSRIVTVGGAEVHLTPNEYGLLE
                     QLVTNAGRVLTHRTLLERVWGPEYTEEVHYLRPVVTSLRKKLGPQLIQTEPGVGYRLK
                     AIDP"
     gene            1654..2031
                     /locus_tag="VH419_04720"
     CDS             1654..2031
                     /locus_tag="VH419_04720"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY7042952.1"
                     /translation="MIVEDEPTLRHIIARNLTSRGVMVQEAETAGEAVQHVLAERPDL
                     LLLDVNLPDRSGWDVLRDLRRQGIEVPTIVISAVYCSPSRLEEFRPLAYLHKPFPLEA
                     LLRLVIGPAEESTDDETEAERLD"
BASE COUNT          432 a          684 c          779 g          432 t
ORIGIN      
        1 ctgctcgaca tgtcgcggct cgacgccggc gccctgcagc cgcggctgga gtgggtgtcc
       61 ctcagcgacg tggtggccga cgtgctcgac cgtatgaccc caatgctcgc cgggcaccag
      121 gtgctggtgc tggtcccgga tacgctgcca gcgacgccga tggacttcgt gctcatcagc
      181 caggtgctca cgaacctgct cgacaatgct gttcgttacg cacctcgggg ggctgccatc
      241 acgatctccg gggagattgt gcgcgaccaa ttgcgcgtga ccgttttcaa tgagggcact
      301 catctgcccg accaggacgt cgagcgggtc ttcgacaagt tctatcgggt cagcgatgtc
      361 ccgggaggga ccgggcttgg cctggcgatt gcgcgcggca acgtcgaggc gcatcatggc
      421 cacatgtggg ctgagaacgt ggggcagcgg ggcgtctcgt tcatcttcac cttgccctcc
      481 ccgcctcccg acgtcgtgac tcaggagctc gcggtggcga aatcggcatg aagggctcgg
      541 tgacgcggat cttgctggtc gacgacgagt ccgacgttcg caacacggtg cgtcgtggcc
      601 tgcagatcca gggctacgaa gtcgagacgg ctgtcgatgg tgaggaggcg gtgttgaaga
      661 cgcgaggctg gcagccggac gtggtgctca tggacctcgc gatgccgaag atgggtggca
      721 tctcggcgat cgaggagatc cggcactggt cggaagtgcc gatcatcgtg ctatctgtga
      781 tgggcgagga gcgcgagaaa gtccgcgccc tggacagcgg cgccgacgac taccttacca
      841 agccgttcgg catgcaggag ctgagcgccc gcatccgggc cacccttcgc cgccgtgcgc
      901 cagcgccgga gggacgcgtc tataccttcg gcacggtggg cgtggatacc acaagccgta
      961 tcgtcacagt cggcggcgcg gaggtccacc tgacgccgaa cgagtacggg ctgctcgagc
     1021 agctcgtgac caacgccgga cgggtcctca cccaccgcac tctgctggag cgggtctggg
     1081 gaccggagta cacggaggag gtccactacc tccgcccggt ggtgacctcg ctgcgcaaga
     1141 agctcggacc acagctcatt cagaccgaac cgggggtcgg ttaccgtctg aaggccatcg
     1201 atccgtagga cccgatctcc tacagaacga agccgtcgac cattcgccgc atgttctaac
     1261 ggattgttga gagccgtcgt ctcgtttcta acgcctttct aatcccgcga ttgatatggt
     1321 cacaatgctg ggtaatcttc cctacccgga agggtagccg atgtgcggtt tcccgccggt
     1381 gcaataccac cccatagtgg ggggtaggtt gctcgctgtc gaggttacac cgggacaaag
     1441 ggggcagaac ggttgcgatg aggacgtaga gagcgtgttg agcaaagtcg gcttctcgtt
     1501 gacatctcgt tgagcagacc ggcgatagac tcgtgttgag gcagtcacga gcttgtgtca
     1561 gatggccatt cagacgcagg ccaaaggcaa gatcaaatga tgccaactct ggcaatgacc
     1621 ggtgaaaggc gggagatcgt gcaccgtcac gtgatgatcg tcgaggacga accgaccctg
     1681 cgccacatca tcgcgcgcaa tctgaccagt cgaggtgtga tggtgcagga ggccgaaacc
     1741 gccggtgaag ctgttcaaca cgtcctcgcc gagcgaccgg atctcctgct gctcgacgtg
     1801 aacctcccgg atcgaagcgg atgggacgtg ctccgcgatc tgcgccgaca gggaatcgaa
     1861 gtaccgacga tcgtgatctc ggccgtgtac tgtagcccga gtcggctgga ggagtttcga
     1921 ccgctggcct atttgcacaa gccgttcccg ctcgaggcgc tgctgcgtct cgtcatcgga
     1981 ccggccgagg aaagcaccga tgacgaaacg gaggcagagc gtttggacta agtacagcat
     2041 ccgggtgtat ggcgtcaggt ttgcgggttg cggaggtggg tgatgggaac attggaatac
     2101 atcgcgagtg gagcgatcgc agtcgccgtg cttgtttacc tggtctacgc gctccttcgc
     2161 cccgagcggt tctagaaggg tggccgagga tgtccgtgga cgctagtgat gagatgactg
     2221 gcgatcgtgg tggtcccctt tggtccacca cgaaccgacc gcacggcgag gcaaccacgg
     2281 gccgcgggat agcggcaccg tggatccatc acctgcgctc gaactgc
//
LOCUS       DATBFZ010000157         8276 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_4039350, whole genome shotgun sequence.
ACCESSION   DATBFZ010000157 DATBFZ010000000
VERSION     DATBFZ010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 8276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8276
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4039350"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            195..1997
                     /locus_tag="VH419_04725"
     CDS             195..1997
                     /locus_tag="VH419_04725"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEY7042953.1"
                     /translation="MRASREPSIARRIHPPSPRRRIRRPTVIAISLTVAVAGIWVDGP
                     AAVAHAAPGSQSTATPHPVRPSIHNVSIPNVAPVDRERVAERSGSRAALAATSVGATI
                     RGAGAAVLVAHVRTSVSQGSSMLGVTWRHGSVDPSKVSVVVRTRLGSAWSPWSAVDVD
                     ADGSSAAEDDTVRDGTVPVFVGAARSVEVDVYSTTRRPPSELSIAEIDPGSSAYDERL
                     AALSRTSDAANRTATSGGATTTAAASVRDPYPQMPQIVTRREWGADESLGDKCWAPRY
                     GRTFKVVFVHHTAGSNTYTRQESPAIVRGILAYHTQTRGWCDIGYNFLVDRYGTVYQG
                     RAGGIRQAVRGAHAGDYNVDSTGISLMGEFTSEMPTRAMRRSLVALTAWRLGVAYHGG
                     YGKGRINGHAFKRISGHRDAMSTACPGQRVYDWLPTLRSRVQARLDHFMSPLKRRWIA
                     MGGFGSRLGPVRVGEVRDNGGMHTTFVNARAYFKHGVLRSLPAGPLLSSYVRSGETNG
                     PLGYPTTRQLSPLNGAAAKFEHGALYWSPATQSRLLKGGKVLHRYRSLNGPNGPLGFP
                     KTHFRNTKVSRFVRFQHGTITYDKSTGRVAVTYT"
     gene            1994..3718
                     /locus_tag="VH419_04730"
     CDS             1994..3718
                     /locus_tag="VH419_04730"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HEY7042954.1"
                     /translation="MNSSPLLTRSGLCATVVTASVVASLSLLDSSAIAGQTYYVPISK
                     KWSVTGHGYGHGHGMSQYGAQGAALQGLNYRQIVDFYYPRTRWATAKGSVRVLISDDY
                     TSDLQVRTRPGLEVRDLADHTAWTLPANDGVDRWRLAARGGATVVQFHDRSGWHRWRV
                     PGRGRFAGAGEFLARGPLTLLLPDGGDSVTPRKYRGTLRSVPPYAGATTRDTVNVLSM
                     DAYVQGVVPYEMPASWKPAALRAQAVAARTYAAWQRAQNPRRYYQICDTTACQVYGGV
                     PAEQSSSNKAVQVTAGKILTYHGRPAFTQFSSSSGGWTSSGGVPYLPAQADPYDAFDD
                     NPMHTWKVTVDVSALERAHPEVGRLVDLRVSSRDGHGSWGGRVQQIVLRGSDGTAYLT
                     GDDFRWQYGLRSTWFAIAPTPIIERWRHIGAAKSSVGRPRSGEYGLVDGSAQKFAHGR
                     IFWSRASGAREMLGPVLRAYRKLGGPSSTLGWPETGVLRAALGGHKVRLQGGYLFAKP
                     KIGAHALFGPILRYWWRHEGGPRGQLGYPTRNVVAVRVGQRATFQGGRITYVRATHEF
                     RVVINNAG"
     gene            complement(3744..4577)
                     /locus_tag="VH419_04735"
     CDS             complement(3744..4577)
                     /locus_tag="VH419_04735"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEY7042955.1"
                     /translation="MLRLTVCSALAVLVAAAVSAPAVTTPAAGTRGRTMQPPGSHAGP
                     PYRFTTELMGQFPLIPLKDQAMLTRTEHGYLFRTGQQDSHLVVTLVDGRLRFVDTGTK
                     RFKKLSPHCHRKSVRVGVAAKCGVPADVTVRRPLLIEVWPRLGNDYTDTSSLPATFAV
                     TVLSDEGHDVAHLGAGPDFFNGHLGRDVVFGGAGNDWIRPGHGNDLVHAGPGNDDIVA
                     MPGKDTLHGGPGRDQLWGGDGSDRIWGDGGADYALCGPGRDRTNADGNDRVAQSCESV
                     D"
     gene            complement(4771..8172)
                     /locus_tag="VH419_04740"
     CDS             complement(4771..8172)
                     /locus_tag="VH419_04740"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HEY7042956.1"
                     /translation="MFAKVLVANRGEIAVRAFRAAYELGAKTVAVFPHEDRRSEHRLK
                     ADEAFEIGERGHPVRAYLDPDAIVRAAVHAGAEAVYPGYGFLSENPALAEACVAAGIS
                     FVGPSADILRLTGNKATAVDAARSAGVPVLSNVAPSRDVDEICASASQLTFPVFVKAV
                     AGGGGRGMRRVDPSTSSGQAIRDLRDAVESAMREADGAFGDPTVFVEEAVDDPRHIEV
                     QILADGQGDIVHLFERDCSLQRRHQKVVEIAPAPRLDTELRDRICADAVAFARTIGYL
                     NAGTVEFLVSPDGRHFFIEMNPRIQVEHTVTEEVTDVDLVQAQLRIASGESLTDLGLR
                     QADIAVRGAALQCRITTEDPANGFRPDTGRITAYRSPGGAGIRLDGGTTYTGAEVSAH
                     FDSMLTKLTCRGRTFEIAVHRARRAVAEFRIRGVATNIPFLQAVLDDPDFRAGGVTTS
                     FIEQHPQLLTARSSADRGTRLLHYLADVTVNRPYGESPTVIDPATKLPPLPSSPPPDA
                     SRQRLQALGAEQFAADLRAQQAVAVTDTTLRDAHQSLLATRLRTRDLVSVAPHLATLT
                     PQLLSVECWGGATYDVALRFLAEDPWERLAVLREALPNVCLQMLLRGRNTVGYTPYPT
                     EVTDAFVDEAAETGIDIFRIFDALNDVDQMRAAIDAVRGTPHGVAEVALCYTGDLSSP
                     AESVYTLDYYLALAERIVDAGAHVLAIKDMAGLLRAPAARTLVTALRGRFDLPVHLHT
                     HDTPGGQLATLMAAVDAGVDAVDAASASLAGTTSQPALSALVAATDQTDRATGLSLQA
                     VCDLEPYWEATRRLYAPFESGIPAPTGRVYRHEIPGGQLSNLRQQAIALGLGEKFEQI
                     EDMYAAADDILGHIVKVTPSSKAVGDLALALVATGADPKDFAENPGGYDVPDSVLGFL
                     AGELGDPPGGWPEPFRTKALAGRAARPREEKLEADDLAALESDRRTTLNRLLFPGPAK
                     EFADSRERYSDLSVLPTRDFFYGLDFDEDHDVYLEEGVRLILGVESISQPDERGFRTV
                     MCTINGQLRPVSVRDRSVESDAPARERAAAGVPGQIGAPFDGVVSVVVGVGAGVEAGQ
                     TVATIEAMKMEAAITSPISGTVERVTLDGPRTVEGGDLVVVISPS"
BASE COUNT         1315 a         2799 c         2851 g         1311 t
ORIGIN      
        1 ggcacgaggg aaacgcgcgg gaaatgacat gggagtaatt acagtggcag attctaagaa
       61 tcgatcagtg gtatggcaga gcgtgcattt cccgtcacat aatcggttct cagttgtcac
      121 acaaacaaca ctgagaacac atgtcacaga gccgtgacgc acgaacgccg aatgttgtcg
      181 ccaggagtcc acccatgcgt gccagccgcg agccttccat cgcccgccgc atccaccctc
      241 cgagccctcg tcgccgaatc cgccgcccca ccgtcatagc catctccctg accgttgccg
      301 tcgccggcat ctgggtcgat ggcccggcgg ccgtggcgca tgccgcgccc ggctcgcaga
      361 gcacggcgac gccgcatccg gtgcggccgt ccatccacaa cgtgtccatc ccgaacgtgg
      421 cgccggtcga tcgggagagg gttgccgagc gtagcggctc gcgggcggcg ctggccgcca
      481 cctccgtcgg cgcaacgatc agaggtgctg gggctgcggt actcgtcgcg cacgtgagaa
      541 cgtcggtttc ccaaggctcg tccatgctcg gcgtgacctg gcggcacggc agcgtcgatc
      601 cctcgaaggt ctcggtcgtc gtacggaccc ggctcggctc cgcgtggtcc ccttggtccg
      661 cggttgacgt cgatgccgac ggatcttcag ctgcggagga cgacaccgtt cgcgacggca
      721 ccgtaccggt cttcgtcggt gctgctcggt ccgtcgaggt cgacgtctac tcgacgacac
      781 ggcggccgcc gagcgagctc agcatcgctg agatcgatcc gggctcctcg gcgtacgacg
      841 aacgcttggc cgcgttgagc cgcacctcgg atgcggcgaa ccgaacggcc acgtcaggcg
      901 gcgcaacgac gactgcggcg gcctcggtcc gagaccccta tccgcagatg ccccagatcg
      961 tcactcggag ggagtggggg gccgacgaga gcctcggcga caagtgctgg gctccgcgct
     1021 acggccgcac gttcaaggtc gtgttcgtgc atcacacggc gggcagcaac acgtacacca
     1081 ggcaggagtc accggccatc gtgcgcggca tcctcgccta ccacacccag acccggggct
     1141 ggtgcgacat cggctacaac ttcctggtcg accgctacgg caccgtctac cagggccgcg
     1201 ccggcgggat ccgccaggcc gtccgcggcg cccatgcggg cgactacaac gtcgacagca
     1261 ccggcatctc gttgatgggt gagttcacca gcgagatgcc gacgcgggcg atgcgccggt
     1321 cgctcgtcgc gctgaccgcc tggcgccttg gtgtcgcgta ccacggcggc tacggcaaag
     1381 gccggatcaa cggtcatgcg ttcaagcgga tctcagggca tcgggacgcg atgtcgactg
     1441 cctgccccgg ccagcgtgtg tacgactggc tcccgacgct gcgcagtcgc gtgcaggcgc
     1501 ggctcgacca cttcatgtcg ccgctcaagc gccggtggat tgccatgggt ggcttcggca
     1561 gccgactcgg tccggtgcgg gtcggcgagg tccgggacaa cggcggcatg cacacgacat
     1621 tcgtgaatgc gcgcgcctac ttcaagcacg gcgtgctgcg cagcctgccg gcgggaccac
     1681 tcctttcgtc gtacgtcaga tccggcgaga cgaacgggcc gttgggatac ccgacgaccc
     1741 ggcagctgag tcctctcaac ggtgcggccg cgaagttcga gcacggcgcc ctctactggt
     1801 ccccggccac gcagagccgg ctgctgaagg gcggcaaggt gctgcacagg taccggtcgc
     1861 tgaacggtcc gaacggtcca ctcggcttcc cgaagacgca cttccggaac accaaggtca
     1921 gtcgtttcgt caggttccag cacggcacca tcacctacga caagagcaca ggacgggttg
     1981 cggtgacgta cacatgaaca gttcgccgct tctcacgcgg tccggtctgt gcgcaaccgt
     2041 cgtgacggca tcggtcgtcg cgtcgttgtc tctcctcgac tcgtcggcca ttgccggcca
     2101 gacctactac gtgcccatct ccaagaagtg gtcggtcacc ggccacggct acggacatgg
     2161 ccatgggatg agccagtacg gcgcccaggg tgcggcgttg caaggtctca actaccggca
     2221 gatcgtcgac ttctactacc cgcgcacgag atgggcgacg gcgaaaggat cggtccgggt
     2281 cctcatcagc gacgactaca cgtctgacct gcaggtcagg acccgtcccg gtctcgaggt
     2341 tcgcgacctc gcggatcaca ccgcgtggac gctgcccgcg aacgacggcg tcgaccggtg
     2401 gcggctcgcc gcgaggggcg gcgcaaccgt cgtgcagttc cacgacaggt cgggatggca
     2461 tcggtggcga gtcccggggc gcggacggtt cgccggcgcc ggtgagttcc tcgcgcgtgg
     2521 accgctcacg ctgctgctgc ccgacggcgg cgactcggtg acgccgcgca agtatcgcgg
     2581 gacgttgcgc tccgtcccgc cgtacgccgg cgcaacgacc cgggacaccg tcaacgtgct
     2641 ctcgatggac gcctacgtcc aaggggtggt cccgtacgag atgccggcgt cgtggaagcc
     2701 tgctgcgctg cgagctcagg ccgtcgctgc acgcacgtac gcggcgtggc agcgggccca
     2761 gaacccgcgc cgctactacc agatctgtga caccactgcg tgtcaggtct atggcggcgt
     2821 accggccgag cagtcctcca gcaacaaggc ggtgcaggtc accgccggca agatcctcac
     2881 ctaccacggc cgacctgcgt tcacccagtt ctcgtcgtcg tcgggtggct ggacgtcgtc
     2941 cggtggcgtt ccgtatctgc ccgctcaggc cgatccgtac gacgccttcg acgacaaccc
     3001 catgcacacc tggaaggtca ccgtcgacgt ctccgcactg gagcgcgcac atcccgaggt
     3061 cggcaggctc gtggacctgc gcgtcagttc acgggatggg cacggcagct ggggtggccg
     3121 ggtacaacag atcgtgttgc gcggatcaga tggaacggca tacctgacgg gtgacgactt
     3181 tcggtggcag tacgggctgc ggtccacatg gttcgcgatc gcgccgacac ccatcatcga
     3241 acggtggcgg cacatcgggg ccgcgaaatc gtcggtgggc cgtccgcgct cgggcgagta
     3301 cgggctcgtc gacgggtccg cacagaagtt cgcacacggc cggatcttct ggtctcgggc
     3361 gagcggtgca cgcgagatgc tcggtcctgt cctgcgggcg tatcgcaagc tcggtggccc
     3421 gtcgtccacg ctcggctggc cggagacggg cgtactgcgg gcggccctcg gtggacacaa
     3481 ggtccgtctc caaggcggct acctgttcgc gaagccgaag atcggcgcgc atgccctctt
     3541 cggtcccatt cttcggtact ggtggcgcca cgagggcgga ccgagaggac agctgggcta
     3601 tccgaccagg aacgtcgtgg cggtccgcgt ggggcagcgt gcgaccttcc agggcgggcg
     3661 tatcacctat gtccgggcga cgcacgagtt ccgcgtcgtg atcaacaacg ccggctgagc
     3721 tgtgcagctt ggccggtcag cgctcagtcg acggactcgc aggactgggc gacacggtca
     3781 ttgccatcgg cattggtgcg gtcccggccc gggccgcaca gagcgtagtc cgcccccccg
     3841 tcgccccaga tcctgtccga accgtccccg ccccagagct ggtcgcgacc gggaccgccg
     3901 tgcagggtgt ccttgccagg catggcgacg atgtcgtcgt tgcccgggcc ggcgtggacg
     3961 aggtcgttgc cgtggcctgg cctgatccag tcgttccccg cgccgccaaa aaccacgtcg
     4021 cgaccgaggt ggccgttgaa gaagtccgga ccggccccca agtgggcgac gtcgtggccc
     4081 tcgtcactca agacggtcac agcgaacgtg gcggggaggg aggaagtgtc cgtgtagtcg
     4141 ttgccgagtc gcggccacac ttcgatcagg agcggtcgcc ggacagtgac gtctgcaggt
     4201 actccgcact tcgccgcgac gccgaccctg acgctcttcc ggtgacagtg gggcgacaac
     4261 ttcttgaacc tcttcgtgcc ggtatcgacg aaacgcagcc ggccgtcgac caaggtcacc
     4321 acgaggtggc tgtcctgctg gccggtacgg aagagatagc catgctcggt ccgggtgagc
     4381 atggcctggt ccttcaacgg gatgagcggg aactggccca tgagctctgt cgtgaacctg
     4441 tacggcggac ccgcgtggga accgggtggc tgcatggtcc tgccacgggt ccccgcggcg
     4501 ggagtggtca cggctggcgc ggaaacggcc gctgcaacga gcacggcaag cgccgagcac
     4561 acggtcagtc gcagcattac gtcctccttg cttcggcgtg gcaggcggcc gcggtcgacc
     4621 aacggtcgcg tggctgcttg ttcccggccg gttcggaccg aaccggtcct ggcatcgcag
     4681 tcggcatgag gcaaaatcgt gattttccgt aactagatac taactgattg ttacctagct
     4741 ggtcggacgg tcactccgtg ggggactccg tcaggagggg gagatcacga cgacgaggtc
     4801 gccaccttcg acggtgcgcg gcccgtccaa cgtcacccgc tcgaccgttc ccgagatcgg
     4861 ggacgtgatg gccgcctcca tcttcatcgc ctcgatcgtg gcgaccgtct gaccggcttc
     4921 gacgcctgcc ccgacgccga cgacgaccga cacgacaccg tcgaacggtg ctccgatctg
     4981 gcccggcacg ccggccgcgg ctcgctcgcg cgcgggagca tcggactcga ccgagcggtc
     5041 gcgcaccgac acgggacgca gctggccgtt gatcgtgcac atgacggtac ggaagccgcg
     5101 ctcgtcaggc tggctgatcg actcgacgcc gaggatcagc cgcacgcctt cctcgaggta
     5161 cacgtcgtgg tcctcgtcga agtcgagacc gtagaagaag tcgcgggtgg ggagcaccga
     5221 caggtcgctg taccgttcgc gcgagtccgc gaactccttg gccgggcccg ggaacaggag
     5281 ccggttcaag gtcgtacgcc ggtcggactc cagcgccgcg aggtcgtcgg cctcgagctt
     5341 ctcctcacgt gggcgtgccg cgcggccggc gagagccttg gtgcggaacg gttccggcca
     5401 accacctggc gggtcgccga gctcgcctgc caggaagccc agcacggagt caggtacgtc
     5461 gtagccgcct gggttctcgg cgaagtcctt cggatcggcg ccggtcgcta cgagggcgag
     5521 cgcgaggtct ccgacggcct tgctcgacgg ggtgaccttg acgatgtgac cgaggatgtc
     5581 gtcggccgcg gcgtacatgt cctcgatctg ctcgaacttc tcgccgagcc cgagcgcgat
     5641 cgcctgctgg cggaggttcg acagctggcc accggggatc tcgtgccggt agacccgccc
     5701 ggtgggcgcg gggatcccgg actcgaacgg cgcgtacagc cgacgcgtgg cctcccagta
     5761 cggctccaga tcgcacaccg cctgcaacga caggccggtc gcgcgatccg tctggtcggt
     5821 cgctgcgacg agcgcggaca gtgcgggctg gctggtcgtt cccgctagcg aggcactcgc
     5881 ggcgtcgacg gcgtcgacac cggcgtccac ggcagccatc agggtggcca gctggccgcc
     5941 aggtgtgtcg tgcgtgtgca ggtgcaccgg caggtcgaag cgccctcgga gtgcggtgac
     6001 gagggtgcgc gccgccggtg cccggagcaa ccccgccatg tccttgatcg cgagcacatg
     6061 cgcgccggca tcgacgatcc gctcggccag ggcgaggtag tagtcgagcg tgtacaccga
     6121 ctcggccggg ctgctgaggt cgccggtgta gcacagcgcg acctccgcga caccatgcgg
     6181 tgtgccccgg accgcatcga tggcggcgcg catctggtcg acgtcgttga gtgcgtcgaa
     6241 gatacggaag atgtcgatgc ccgtctcggc tgcctcgtcg acgaaggcgt cggtcacctc
     6301 ggtggggtag ggggtgtagc cgaccgtgtt gcgcccacgc agcagcatct gcaggcagac
     6361 gttgggcagc gcctcccgga gcaccgcgag ccgctcccac gggtcttcgg cgaggaagcg
     6421 gagcgcgacg tcgtacgtcg ccccgcccca gcactcgacc gagagcagct gcggtgtcag
     6481 cgtcgccagg tgcggcgcca ctgacaccag atctctcgtc ctcagccggg tcgccaacag
     6541 cgactggtgg gcgtcccgca gcgtcgtgtc ggtcacggcg accgcctgct gcgcacgcag
     6601 gtcggcggcg aactgttcgg cgcccagagc ctgcagccgc tggcgcgacg catcgggtgg
     6661 cggcgaggag ggcaagggcg gcagcttggt ggccgggtcg atcacggtcg gcgactcgcc
     6721 gtacggccgg ttcacggtga cgtcggcgag atagtgcaag agccgggtgc cgcggtcggc
     6781 cgacgaacga gcggtcagca gctgcgggtg ctgctcgatg aacgacgtcg tgacgccacc
     6841 ggcgcggaag tccgggtcgt cgagcacggc ctggaggaac gggatgttcg tggcgacgcc
     6901 acgaatccga aactccgcaa cagcccgtcg cgcccggtgc accgcgatct cgaacgtgcg
     6961 gccgcgacag gtcagcttcg tcagcatcga gtcgaagtgg gccgacacct cggctccggt
     7021 gtacgtcgtc ccgccgtcca gccggatacc cgcgccgccc ggcgagcggt acgccgtgat
     7081 ccgaccggta tccggacgga agccgttcgc aggatcctcc gtcgtgatcc ggcactggag
     7141 cgcggcgccg cggacagcga tgtccgcctg gcggagaccg aggtcggtga gtgactcgcc
     7201 ggaggcgatg cgcagctgcg cctggacgag gtcgacgtcg gtcacctcct cggtgacggt
     7261 gtgctcgacc tggatgcgcg ggttcatctc gatgaagaag tggcggccgt cagggctgac
     7321 gaggaactcg acggttcccg cattgagata gccgatcgtg cgcgcgaacg cgaccgcgtc
     7381 agcacagatg cgatcacgca gctcggtgtc caggcgtggg gcgggcgcga tctcgacgac
     7441 cttctgatga cgccgctgca gcgaacagtc gcgttcgaac aggtggacga tgtcaccctg
     7501 tccgtcggcc aggatctgca cctcgatgtg gcgcggatcg tcgacggcct cctcaacgaa
     7561 gacggtcggg tcgccgaacg cgccgtccgc ctcccgcatc gcggactcaa cagcgtcgcg
     7621 caggtcgcgg atcgcctgtc ctgagcttgt cgaagggtcc acccggcgca tcccgcgccc
     7681 gccgccaccc gccaccgcct tgacgaacac cggaaacgtg agctgcgatg cgctggcgca
     7741 gatctcgtcg acgtctcgtg aaggtgcgac gttcgagagc acgggtacgc cggctgacct
     7801 ggccgcgtcc accgcagtcg ccttgttgcc ggtcaaccgc aggatgtccg ccgacgggcc
     7861 gacgaagctg atgccggcag cgacacacgc ctcggcaagt gcaggattct cggagaggaa
     7921 gccgtacccg ggatagaccg cttcggcgcc tgcgtgcacg gctgcgcgga cgatcgcgtc
     7981 agggtcgagg taggcccgca ccggatgccc acgctcaccg atctcgaagg cctcgtccgc
     8041 cttcagccgg tgctccgagc gacggtcctc gtgagggaag accgcgacgg ttttcgcgcc
     8101 gagctcgtac gccgcccgga acgcgcgaac ggcgatctcg ccccggttgg ccaccagcac
     8161 cttcgcgaac atgctcacaa ccctaggaga ctgctgaaga atcatgctct gctgcgcacg
     8221 cccgaggcgg cgatccgcgg cgttgtcgtc ggtcgacggg ggtcttcacc ccgcct
//
LOCUS       DATBFZ010000158         2901 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_1088391, whole genome shotgun sequence.
ACCESSION   DATBFZ010000158 DATBFZ010000000
VERSION     DATBFZ010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2901
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1088391"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1059
                     /locus_tag="VH419_04745"
     CDS             <1..1059
                     /locus_tag="VH419_04745"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY7042957.1"
                     /translation="KLDALTGTGVAAQREQLQSQLVAAQERLKSDAAKLDQVLAGPQQ
                     EEIDAAAAAVRQAEQQLALAVVPSTEDDIRAQRALVDQARQQLEKARLPAAPADIQQQ
                     RETVAEREAVLQSKANPFTVNDLNAAQAAVDQARSQVAVAQGTLDQTIVVAPFDGVIS
                     QRLLAPGSFATTTTPIVALVGADIETHVTVEEARLASFAPGQTVALEVPAYPGVLFPG
                     RVTAVSPTGDTRAHTFDVTIVPTGSDQRLRPGMFAQVDLTVSEKDEAVLVPREAVVQQ
                     DGRSVVFVVQDGRARARPVETGISSDQGIEIVSGVTPGDRVVVVGQQGLRDGSAVRLP
                     EAQTQGEGQRPQGQRRQQ"
     gene            1166..>2901
                     /locus_tag="VH419_04750"
     CDS             1166..>2901
                     /locus_tag="VH419_04750"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY7042958.1"
                     /translation="MGILALVLLGAVSYSYLKVDRLPPLSFPIVNVTIQYPQAAAQDV
                     ETLVTKPVEDALAGIPGAQTISSTSSEGRSQVRVQFVEGANMDISALDVERRVSRIRG
                     RLPVDAGDPSIAKADPNEFPIMNVALTGAPMDQLRDLATTQLQPALQSVLGVASVNIS
                     GGLVREVQVTLDYSKLAAYNLTAQQVATALTSANVTSTVGSYEDSGRLFSMRAIGNFQ
                     TPEQMGNLVVAQTTGGPVLLRDLATVSPGYKDLREIQRINGEDAVGISIVKASDANAL
                     QVADDVRAAMAAIQPTLPAGTRVLIRNDASIFTRASLHAVQFDLMLAVIIVGFITFLF
                     LHSFRNVLIILLSIPTSMISTFLVMFAMGFSLNLMTLMALALMVGILVDSSIVVVENI
                     HRHLQLGKEPREAALVGRNEIGLATIAIAAADVVVYVPIAFMSGGVGQLFRQYGLTVV
                     AATIFSVIISFTLTPMLASRWMKHDHEGKGPIARFGAWWDARFDVLARIAQRTVPIAV
                     KARWGVVLVAVVLVAASVMLVQTRAIGSEYAPQEDNDNFQVIMTTPPGTSLLATDAAT
                     KQFEEALQAIPEV"
BASE COUNT          498 a          954 c          954 g          495 t
ORIGIN      
        1 aagctcgacg cgctcacagg caccggcgtt gccgcccaac gggagcaact ccagagccag
       61 ctcgtcgctg cccaggagcg gctgaagagc gacgccgcca agctcgacca ggtgctcgcc
      121 ggccctcagc aagaggagat cgacgctgcg gcggccgccg tgcggcaggc agagcagcag
      181 ttggcgctcg ccgtggtgcc gtcgaccgag gacgacattc gagcgcagcg cgcactcgtc
      241 gatcaagcgc ggcagcagct cgagaaagca cgcctgcccg ccgcgcccgc cgacatccag
      301 cagcagcgcg aaacggtggc cgagcgcgag gctgtgctgc agagcaaggc caacccgttc
      361 accgtcaacg atctgaatgc ggcgcaggct gctgtggacc aggctcgatc tcaggtcgcg
      421 gtcgcgcagg gcaccctcga tcaaaccatc gtcgtagcgc cgttcgacgg ggtcatctca
      481 cagcgcctgc tggcgccggg gtccttcgcg acgaccacga ctccgatcgt ggcgctcgtt
      541 ggcgctgaca tcgagaccca cgtgacagtg gaagaggcac ggctcgcgtc cttcgccccc
      601 ggacagacgg tggcgctgga ggtgccggcg tatccggggg tgctcttccc cgggcgcgtc
      661 accgcggtct cgccgaccgg agacacgcgc gcacacacat ttgacgtgac gatcgtgccc
      721 accggatcgg accagcggct ccgccccggc atgtttgccc aggttgacct gacggtgtcc
      781 gagaaggacg aggcggtgct ggttccgcgc gaggccgtcg tgcagcagga cgggcgttcg
      841 gtcgtgttcg tcgtccagga tggtcgagcc cgggcgcgac cagtcgagac cggtatctcg
      901 agcgatcagg ggatcgagat cgtgagcggc gtcactcccg gtgaccgggt ggtggtcgtc
      961 ggccagcagg gtttgcggga tgggtccgcg gtacgattgc ccgaggccca gacgcaagga
     1021 gagggccagc ggccgcaggg acagcggcgg cagcagtagc agcgagagca tcgggattga
     1081 atcttcgcag gtggcgtttg cgactgcgta gggaggatca accggaatga cacgactttc
     1141 gatcgagcgc ccgctcaccg ttcttatggg gatcctggcg ctcgtcctcc tcggtgccgt
     1201 gtcgtacagc tacctgaaag tggatcggct gcccccgctt agctttccaa tcgtcaacgt
     1261 gacgatccag tatccgcagg ccgcagcaca ggacgttgaa acgctcgtca cgaagccggt
     1321 cgaggatgct cttgcgggca tccccggggc gcagacgatc agctcgacgt cgagcgaggg
     1381 tcgctcccag gtacgggtgc agttcgtcga gggcgcgaat atggacatct cggcactcga
     1441 cgtcgagcgc cgcgtgtcgc gcatccgtgg ccgcctcccc gtcgacgccg gcgatccgtc
     1501 gattgccaag gcggacccga acgagttccc gatcatgaac gtcgccctca cgggcgcgcc
     1561 catggatcag ctgcgtgatc tcgcgacgac ccagctccaa ccggcactgc agtcggtgct
     1621 gggcgtagcg agcgtgaata tcagcggcgg ccttgtgcgc gaagtgcagg tgacgctgga
     1681 ctattcgaag ctcgccgcat acaacctcac ggcacagcag gtagccacgg cgctgacctc
     1741 ggctaacgtg acgtcgaccg tcggaagcta cgaagacagc ggccgccttt tcagcatgcg
     1801 ggccatcggc aacttccaga cgcccgaaca gatggggaac ctggtcgtcg cccagacgac
     1861 gggcgggcct gtcctgctgc gtgacctcgc aacggtgtcc cccggataca aggatctccg
     1921 ggagatccag cgcatcaacg gcgaagatgc ggttggtatc tccatcgtca aggcgtcgga
     1981 cgccaacgcc ctccaggtcg cggacgatgt ccgcgcggcc atggcggcga ttcagcccac
     2041 gctgccggcg ggtacgcgcg ttctcattcg gaacgacgcg tcgatcttca cgcgcgcgtc
     2101 gctgcacgcc gtgcagttcg acctcatgct ggcggtcatc atcgtcggct tcatcacctt
     2161 cctgttcctg cactcgttcc gcaacgtgct gatcatcctg ctttcgatcc cgaccagcat
     2221 gatcagcacg ttcctggtca tgttcgcgat ggggttctcc ctcaacctga tgaccctcat
     2281 ggcattggcg ttgatggtgg gtatcctcgt ggacagctcg atcgtcgtcg tcgagaacat
     2341 ccaccgtcac ctccagctcg gcaaggagcc ccgtgaggcg gcgctggtcg gccgcaacga
     2401 gatcggactc gcgacgatag cgatcgccgc cgccgacgtc gtggtgtacg taccgatcgc
     2461 gttcatgtca ggcggtgttg ggcagctctt ccggcagtat ggactcaccg tcgtggccgc
     2521 gacgatcttc tccgtcatca tctcgttcac ccttacgccg atgctggcgt cgcgctggat
     2581 gaaacacgac cacgagggaa agggccccat cgcgcgcttc ggtgcctggt gggacgcacg
     2641 cttcgacgtt ctcgcgcgca tcgcgcagcg aaccgtgccc atcgcggtga aagcgcgctg
     2701 gggtgtcgtg ttggttgctg tggtgctggt cgcggcgagc gtcatgctcg ttcagacccg
     2761 cgcaattggc tccgagtatg ctccccagga ggacaacgac aacttccagg tgatcatgac
     2821 gacaccgccc gggacctcgc tcctggcgac cgacgctgcc accaagcagt tcgaggaggc
     2881 cttacaggcc attcccgaag t
//
LOCUS       DATBFZ010000159        12261 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_102361, whole genome shotgun sequence.
ACCESSION   DATBFZ010000159 DATBFZ010000000
VERSION     DATBFZ010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 12261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12261
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102361"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            152..820
                     /locus_tag="VH419_04755"
     CDS             152..820
                     /locus_tag="VH419_04755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042959.1"
                     /translation="MDVKVQRRSTRRLRAASPAAARAVAAPMTSLLPSVDGSSRQQLV
                     DLQRTVGNRAVTALVAAYDGNGHTPPIRLHGRTFGTFDGGSSSVQQQRVRRATGCDCP
                     AEDPCLRATGSLAIGYHVDVTIEMPPMPDGLSQCQQRRVRTFLRDVLGPHEQEHARRL
                     RTYNGTTRRPFDVTGCGRSGLDTAVTTKLQQMHDDEADARAKTADQLSLAIDPFERDI
                     DLDC"
     gene            944..2491
                     /locus_tag="VH419_04760"
     CDS             944..2491
                     /locus_tag="VH419_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013861272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HEY7042960.1"
                     /translation="MPTYLSPGVYVEEVESGARPLEGVGTAVAAFVGLAEQGPFNAPT
                     LVSNWTQFTEAFGGFVSGSYLAQSVYGYFMNGGGNCYVVRIGQNGSGGGGKGRPKELP
                     AGPQAVVGRFRMAALEGTPAGEISVEITDPGGDAPAQEMFRVVVKRNGEQEEEFDRLT
                     LGRGKSNVVTAVNTASKLIQIEEIAGQGAVERPPNGETSLAEAPPPAAVPTPRLSTDD
                     YVGDVADRTGFGGLEAVDEVTMLCVPDLMSAYQQGAIDLETVQAVQLAMIAHCELMGD
                     RVAVLDAPPGLNAQQVKEWRVDKAGYDSAFATLYYPWIKVADPSTGQLAHMPPSGHVA
                     GIWGRNDDTRGVHKAPANEVVRGAISLEVQLTKNEHDLLNPVGINCIRTFPGRGNRIW
                     GARTLSSDPAWRYLNVRRLFNYLEESILAGTNWVVFEPNDEALWAKIRRTIAAFLVNE
                     WRKGALFGSTPDEAFYVKCDGETNPAEGIDAGQVVCEIGVAPVKPAEFVIFRLSQFSG
                     GTSLVTE"
     gene            2560..3003
                     /locus_tag="VH419_04765"
     CDS             2560..3003
                     /locus_tag="VH419_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013861271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEY7042961.1"
                     /translation="MPLPELDAGVGHSFGLEFDGVQIKSITEISGLKMEQDVIELKEN
                     GPDGKYVIKKLPGRWKAPEVTLTRGISADNSFDKWIKDSQFGKMNDVRKGGAIIVYDY
                     EGTAVKRYKLTNAWPKSLEIGAMKAGDTSVLTEKLVVTCERLEVE"
     gene            3003..3461
                     /locus_tag="VH419_04770"
     CDS             3003..3461
                     /locus_tag="VH419_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013861270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042962.1"
                     /translation="MRRATADAQAAVAATQSSPESPDASEVAESLRTEFAFVLPRGYV
                     DQGGTVHRSGVMRLATARDELVPLRDDRVRENPAYLTVVLLARVVTRLGTLTDIHAGV
                     IENLFAADLAFLQDLYRRINQEGQTLAGVTCPECGHGFAVDVAGGRPGES"
     gene            3458..3616
                     /locus_tag="VH419_04775"
     CDS             3458..3616
                     /locus_tag="VH419_04775"
                     /inference="COORDINATES: protein motif:HMM:NF042798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6760 family protein"
                     /protein_id="HEY7042963.1"
                     /translation="MTYAADQLYGEVAYVAYHFHWSRAEILDLEHPERRRFVDEISQI
                     NARRERGG"
     gene            3662..5890
                     /locus_tag="VH419_04780"
     CDS             3662..5890
                     /locus_tag="VH419_04780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042964.1"
                     /translation="MRTPDAQDLPSRGANAQRTDDPGWRDVPALQRTVHELAPIASYA
                     HFSDDLATRHDPRFLEPLGHELTPDAPAGEVDGLVAPQPAHDLALTTVVYPVPPSRAN
                     RIVSSDTPPTIQRAAAPALVSREASPPGSGVPARESAAQTATPAPPTIQATTETPSMA
                     LPAETPVPDDRSVPAEQPAEEPAEQPVDRTGADSEPQAPAVRQVPVEPRPLVGQTPIS
                     LPEATSPTAATPAPRAPARETTPAPAPAVSRSVDLGGPPPAPVSRAADVAAPAHPPAS
                     PRAVAARSTPTSRLPPQGDPPPSITSITWSDIHETGWTAESARPALEPLSTFEPVPTP
                     APSLPVVARTVSESSPAVPTTGDEAGHPDGGQIESTTLSTTPSVVPLVGEREDAPPIE
                     RPDDLPLTPTDAPSPIDQPTPLPSLRTVEYAGPPPTSFTPPVVSRTTLPTVEVRTRPS
                     TSVQRAVTGASETTPLTSKQQPGLHQPSTPTRPASSETPLTERARPSMELPIVDPVVA
                     TRPVSQALPAIQRVAAVPGADLSPPSHDLAGVVRPAATSAGVRRGAMRESGQPTDPPN
                     RPMRPSAQRVLDPVRTPATPEPGRVSVLPLQRMFADAAPAPQSDAGSAGFAVQRDQEA
                     GTSLVWQRVSGDEGVVAPADRPAEDEPPSSSPAPATPSPPSEPAVASEPAAEAAAPPT
                     EAPAATPAASTPTASGAAAPPSAAELDEMAKRLYEPVSARLRSELWLDLERHGLVTAR
                     SR"
     gene            5903..6346
                     /locus_tag="VH419_04785"
     CDS             5903..6346
                     /locus_tag="VH419_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013861267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEY7042965.1"
                     /translation="MTESYPEPAVTVCFGVTIDDTRLGTFNSCEGLGVEVVLEQREEG
                     GNNGFVWQLPSRLKFSNIKLSRPVCKDTTEVMNWLSSMTKKVEPRTASIKAMTAERKV
                     VATWNLLGVIPVRWSGPALNLDSPKVAIETLEIAHHGFAPAKAGA"
     gene            6343..7113
                     /locus_tag="VH419_04790"
     CDS             6343..7113
                     /locus_tag="VH419_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013861266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HEY7042966.1"
                     /translation="MNQPIALMTSSSGAGVAAPAPSGDPSRPQLERASLKLYDPTPAG
                     GGGGAPGGQRGSIAFQFNPKEVTIAKTAKWERKTAKGAKKAGPPEFSGSEPCKLTMEM
                     FFDASGTQDGSVVQAVEQLFSCCVPTDDSIGKKKPTPPLVVLSWGKVTSFPAFVTSVS
                     AKYTLFSAEGLPIRALCSVALEEMPNEPWRQNPTSGGRSVRRSHTMIEGDTLASVAYA
                     EYGDPTMWRAVAAYNGIDDPMRVAAGRDLLLPAPEELG"
     gene            7119..8912
                     /locus_tag="VH419_04795"
     CDS             7119..8912
                     /locus_tag="VH419_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013861265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VgrG-related protein"
                     /protein_id="HEY7042967.1"
                     /translation="MSITNTFLVDVDGTPLPSDITPMLTAAYVDDSQQLPDMFELRFR
                     DGQHVVLTKTNAKIGSTVRISVMTSTSQSPALLMEGEITALEAEFDHGGTFTVIRGYD
                     AAHRLFRGRRTESYTQMTASDIASKVAQRAGLKTGDISSTTTVFEHVSQAGTSDWELL
                     DSLARQNGYEISVREGKFNFGPPTQAGSAPAAGGAKPQNPLVLQLGTNLLRFRSVVTS
                     AEQVKEVEVRGWDVATKKALTTTVPAATKMAELRDATPAGLAHTFGDPVYVSTDVPHR
                     AQAEVDTAAAALVEEIAGGFAEFEGVVMGNPDVRAGAAVTVDNLGSPFDGKYTVTRSR
                     HRFDPATGYTTAFSVTGVRDRSLLGLTGGAGHPPSAPDGVVIAQVDDVKDPDGQARVK
                     LTFPWLSDTFVSTWARTVQAGAGKDRGATVLPEVGDEVLVAFEQGDFRRPYVIGGLYN
                     GVDTPSTKGVDLVDSSSGAVNRRSLVSRQGHRIDLLDQDGKSEGISITSKDDKVSLLM
                     DSTNTKITVHSDGTVLIEATNGVVVDSKSSKLELKGGQVSISGSNGVTVDGGSGQLQL
                     KGTTASLEGSATTEVKGGSLCSVSAGLVKIN"
     gene            8923..9204
                     /locus_tag="VH419_04800"
     CDS             8923..9204
                     /locus_tag="VH419_04800"
                     /inference="COORDINATES: protein motif:HMM:NF017315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HEY7042968.1"
                     /translation="MPAAARVGDPTGHPGVIGPPGVPTVLIAGQPAATVGTPHICSFP
                     PPAVHPPTAIVPPGCPTVLIGGMPAARMGDMAGCGAPIVMGAPTVLIGG"
     gene            9206..9661
                     /locus_tag="VH419_04805"
     CDS             9206..9661
                     /locus_tag="VH419_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013861264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HEY7042969.1"
                     /translation="MNHDFIGAGWAFPVGTDATGSIALVTREREIEQAIRLVLGTARG
                     ERPMRPEFGCRIHEHVFGSANAATAGQIAYDVREALERWEPRIDVLEVNVGFDQIMVG
                     VLYVDVGYVIVGSNDPRNLVFPFYVIPEQEAPAVVPASTATLRAVGSGV"
     gene            9663..11615
                     /locus_tag="VH419_04810"
     CDS             9663..11615
                     /locus_tag="VH419_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013861263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative baseplate assembly protein"
                     /protein_id="HEY7042970.1"
                     /translation="MLPAPNLDDRTFQGLVDEAKRLVQRRCPEWTDHNVSDPGVTLIE
                     AFAQMVDQLIYRLNRVPDLNYVKFLELIGVELRPPSAARGAVTFWLSAPQPQPVLVRA
                     ETEVATPRTDIHEPVVFTTTQDLEILPCSLSRAGIHPNGAEPSDLTEALSGPQGFACF
                     QATPVPGDALLIGLSDAVPSCAVAIRLDCAVAGGGGVDPRRPPLLWEAWTGTAWVRCD
                     VDSDETGGLNRPGDVIVHLPPQHQTSIIARHRAGWLRCRVVEAEPGQPTYTASPRILS
                     ATAFTIGGTVPMMHAEVVRRELVGRSDGTPGQRFPLQRRPVVPWEEPTTVEIVDETTA
                     DTWQAVADFAASGPDDPHFRIDHYAGEVQFGPSVRLTDGGLRSYGAVPASGASVMVTS
                     YRTGGGRRGNVARGQIRVLKTSVPYVARVENRAPAIGGADAETLDEAKVRGPLLLRSR
                     GRAVTSEDFEELAREVAPDAARVHCVVTEQGAEDGGIRLLVVPHVAGDEIGRIRRQDL
                     IPPDATLERITASLDERRLVGTRLLVAPPEYVGLTAVVDVSARSRFDPDEVRDDVLRA
                     VYQLFDPLTGGPDGGGWPFGRSVQSHEVHAALSRISGVDMAEEISVSLFPADAETGRR
                     SAAVQRLDLPPTALVFSYEHQVRVRR"
     gene            11612..12199
                     /locus_tag="VH419_04815"
     CDS             11612..12199
                     /locus_tag="VH419_04815"
                     /inference="COORDINATES: protein motif:HMM:NF021220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEY7042971.1"
                     /translation="MRGAIDGLVSPHPIGETLPSMYQGDPFTRDVCDALDDVLAPTLC
                     TLDNLAAYLDLSTAPEDLLPWLAGWIGMSLDRGQQPERQRELLRAAGRLQGWQGTARG
                     IELAIEAVLGMRTEVLESGGAIWSLDPNDPVPGEPVPSIVVRVFVGDEHQHAPDVGQQ
                     VDEGRLNAVIEAIKPVHVRHRLQVVTTTQPDQPDT"
BASE COUNT         2007 a         4319 c         4192 g         1743 t
ORIGIN      
        1 gggcggcgat ctgcggcgtt atcgtcggtc aacgatgctg cgcatcgcct ccctcctctg
       61 ccttgcagct catccacccg gacacccgct cacgacgagc aacgttggtt gacgcggcac
      121 tagacagtcg aggcgtccac acggtgcact ggtggacgtg aaggtgcagc gcaggtcgac
      181 gcgtcggctt cgcgcggcgt cgccggcggc ggcccgagcg gtcgcagcgc cgatgacgtc
      241 gctgctgccg tcggtcgacg gctcatcccg ccagcagctg gtcgacctcc agcgcacggt
      301 cggcaaccgt gccgtcaccg ccctcgtcgc ggcgtacgac ggcaacggtc acacgccacc
      361 catccggctg cacgggcgga ccttcggcac cttcgacggc ggcagcagct ccgtacagca
      421 gcagcgggtc cgccgtgcaa ccgggtgcga ctgcccggca gaggacccct gtctgcgcgc
      481 caccggttcg ctcgcgatcg gctaccacgt cgacgtcacg atcgagatgc cgccgatgcc
      541 cgatggtctc tcgcagtgcc aacagcgccg ggtccggacc ttcctgcgcg acgtgctcgg
      601 cccgcacgag caggagcacg cccgccggtt gcgcacgtac aacggcacca cccggcggcc
      661 gttcgacgtg acgggctgcg gacgctctgg gctcgacacc gcggtgacga cgaagctgca
      721 gcagatgcac gacgacgagg cggacgctcg ggccaagacg gccgaccagc tctcgctggc
      781 gatcgacccg ttcgagcggg acatcgacct cgactgctga gggagtgtct gccgctttcc
      841 cgtaaaggca gccgaggttg cccgaggcgg cgtccggtcg gtcgttctgt gaacacacat
      901 cgtggaggca cgctggtctg acaagccgag cgaggagggc agcatgccga cctacctgtc
      961 accgggtgtg tacgtcgagg aagtggagtc gggtgcccgc ccgctcgagg gagtcggcac
     1021 cgctgtcgcc gccttcgtcg gcctcgccga acagggaccg ttcaatgctc cgacactggt
     1081 gagcaactgg acacagttca ccgaggcgtt cggcggcttc gtctcgggct cctacctcgc
     1141 ccagtccgtc tacggctact tcatgaacgg cggcggcaac tgctacgtcg tccggatcgg
     1201 ccagaacggc tccggcggcg gtgggaaggg ccggccgaag gagctccctg cgggaccgca
     1261 agccgtcgtc ggacgcttcc ggatggcggc gctcgaaggt acgcccgcgg gtgagatcag
     1321 cgtcgagatc accgatccag gcggcgacgc acccgcccag gagatgttcc gtgtcgtcgt
     1381 caagcgcaac ggcgagcagg aggaggagtt cgaccgcctc acgctcggcc gcggcaagtc
     1441 gaatgtcgtg accgcggtca acacggcctc gaagctgatc cagatcgaag agatcgcggg
     1501 acaaggtgcg gtggagcggc cgccgaacgg cgagacctcg ctggccgagg cgccgccacc
     1561 ggcggcggtc ccgaccccgc gtctcagcac cgacgactac gtcggcgacg tcgccgatcg
     1621 aaccggcttc ggtggcttgg aggcggtcga cgaggtgacc atgctgtgcg ttcccgacct
     1681 gatgagcgcc taccagcagg gcgcgatcga cctcgagacc gtgcaggcgg tccaactggc
     1741 gatgatcgcc cactgtgagc tcatggggga ccgggttgcg gtcctcgacg cgcctcccgg
     1801 actcaacgcg caacaggtga aggagtggcg cgtggacaag gccggctacg actcggcctt
     1861 tgcgacgctg tactacccgt ggatcaaggt cgccgacccg tcgaccggtc agcttgcaca
     1921 catgccgccg agcggccacg tcgccgggat ctggggacgc aacgacgaca cgcgtggcgt
     1981 ccacaaggcc ccggccaacg aggtcgtccg gggcgcaatc agcctcgagg tacagctcac
     2041 gaagaacgag cacgacctgc tgaacccggt cggcatcaac tgcatccgga ccttccccgg
     2101 tcggggcaac cggatctggg gcgcccggac gctgtcgtcg gacccggcct ggcgctacct
     2161 caacgtacga cgcctgttca actacctcga ggagtcgatc ctcgccggca cgaactgggt
     2221 cgtcttcgag cccaacgacg aagcgctgtg ggcgaagatc cggcggacga tcgccgcctt
     2281 cctggtcaac gagtggcgca agggtgcgtt gttcggatcc acacccgacg aggcgttcta
     2341 cgtcaagtgc gacggcgaga ccaacccggc ggaagggatc gacgccggtc aggtcgtctg
     2401 cgagatcggc gtcgctcccg tcaagccggc cgagttcgtg atcttccggc tgtcgcagtt
     2461 ctcgggcggc accagcctcg tcaccgagtg agaccacctg cccgatcgcc cgcgagatcg
     2521 agcaccggag cagaacgaga agagatgagg tagcaaccga tgccacttcc cgagttggac
     2581 gcaggtgttg gccattcctt cggactggag ttcgacggag tccagatcaa gagcatcacc
     2641 gagatcagcg gtctcaagat ggaacaggac gtcatcgagc tcaaagagaa cgggcccgac
     2701 ggcaagtacg tcatcaagaa gctgcccggc cgctggaagg cgcctgaagt cacgctgaca
     2761 cgcggcatct cggcggacaa cagcttcgac aagtggatca aggactcgca gttcgggaag
     2821 atgaacgacg tccgcaaggg cggcgcgatc atcgtctacg actacgaggg cacggcagtc
     2881 aagcgctaca agctgaccaa cgcctggccg aagagcctgg agatcggcgc catgaaggcc
     2941 ggcgacacct ctgtcctcac cgagaagctc gtcgtcacct gcgaacggct cgaggtggag
     3001 tgatgcgccg agcgaccgcc gacgcccaag cggcagtagc ggcgacgcag tcgtcgccgg
     3061 agtcgccgga tgcgtccgag gtcgccgagt cgttgcgcac cgagttcgcg ttcgtgctgc
     3121 cgcgtgggta cgtcgaccag ggcggcaccg tacatcgcag tggcgtcatg cgcctcgcca
     3181 cggctcgcga cgagctcgtg ccgctgcgcg acgaccgggt ccgcgagaac ccggcgtacc
     3241 tgacggtcgt gctgctcgcg cgcgtcgtga cgcggctcgg cacgctgacc gacatccatg
     3301 ccggcgtgat cgagaacctg ttcgccgccg acctggcgtt cctgcaggac ctgtaccgaa
     3361 ggatcaacca ggaaggccag acactggccg gggtcacctg ccccgagtgt ggccacgggt
     3421 tcgcggtcga cgtggccggt ggtcgcccgg gggaatcgtg acgtacgcgg cggatcagct
     3481 gtacggagag gtcgcgtacg tcgcctatca cttccactgg tcgcgtgcgg agatcctcga
     3541 cctcgagcac cccgagcgac gccgcttcgt cgacgagatc agccagatca acgcccgtcg
     3601 cgagcggggc gggtgaccgc cgatgcgatg gccctggcac cgtgtcgagc atccaggtcc
     3661 ggtgcgcaca ccggatgcgc aggacctacc gtcacgcggc gcgaacgcgc agcgcaccga
     3721 cgatcccggc tggcgggacg tgcctgcgct tcagcgcacc gtccacgagc tcgcaccgat
     3781 cgcgtcgtac gcgcacttca gcgacgatct ggcgacccgg catgacccac gcttcctcga
     3841 gcccctcggc cacgagctga ctcccgacgc cccggcgggc gaggtggacg ggctcgtcgc
     3901 accgcagccc gcgcacgacc tggcgctgac caccgtcgtc taccctgtcc cgccgagccg
     3961 ggccaatcgc atcgtctcgt cggacacccc gccaaccatc cagcgggcgg ccgcccccgc
     4021 acttgtatcc cgagaggcgt ccccgcccgg atccggcgtg ccggcacgtg agtcagcggc
     4081 gcagaccgcc acgcccgccc cgcccacgat ccaggcaacg actgagaccc ccagcatggc
     4141 gctcccggct gagacgccgg tacctgacga ccggtccgtg ccggccgagc agccggccga
     4201 ggagccagcc gaacagccgg tcgaccggac cggagcggac agtgagccac aagccccagc
     4261 ggtccgccag gtgcccgtcg agccacgtcc gctggtgggt cagacgccga tctcgctgcc
     4321 cgaggcgacg tcaccaacgg ctgcgacgcc ggcaccccgt gcccccgccc gggagacgac
     4381 tccggcccct gcccccgcag tgagccgcag cgtcgacctc ggcggacctc cgccggcacc
     4441 cgtcagccgg gccgccgacg tcgccgcgcc ggcgcaccca cccgcctccc ctcgggccgt
     4501 agcggcccgc tcgacaccga cttcccgcct cccgccgcag ggcgacccgc cgccgagcat
     4561 cacttcgatc acctggtcgg acatccacga gacgggctgg accgccgaga gcgcccgacc
     4621 cgcactagaa ccgctctcca ccttcgaacc cgtgcccaca ccagcgccga gtctccccgt
     4681 ggtggcccgc actgtgtcgg agagctcccc ggccgtgccc accaccggcg atgaggcggg
     4741 gcatccggac ggcggccaga tcgaaagcac caccctctcc accacgccgt cggtcgttcc
     4801 gctcgtcggg gagcgggagg acgcgccgcc catcgagcgc ccggacgacc tcccgctcac
     4861 gcccactgac gcaccctcgc cgatagatca gccgacgccg ctgccgagcc tccggacggt
     4921 cgagtacgcc ggacccccgc cgacgtcctt cacgccacca gtcgtgagcc ggacgacact
     4981 gccgactgtc gaggttcgca cccggccctc gacgtcggtg cagcgtgcgg tcaccggcgc
     5041 ttccgagacg acaccactga cgtccaaaca gcagccgggc ctgcaccagc cgtcgacgcc
     5101 gacacgaccg gcatcgtcgg aaacgccact gaccgagcgc gccaggcctt cgatggagct
     5161 gccgatcgtc gacccggtcg tcgcgacacg gcccgtctca caggctttac ccgccatcca
     5221 acgtgtcgcc gccgtgccgg gtgccgacct gagtccgccg tcccacgacc tcgcaggcgt
     5281 cgtccggccg gccgcgacct cggcgggggt gcggcgaggc gcgatgcggg agtccggtca
     5341 gccgacggac ccgccgaaca ggccgatgcg accctcggcg cagcgggttc tcgacccggt
     5401 gcggacgcct gcgacacccg agcccggccg ggtgagcgtc ctgccgctgc agcggatgtt
     5461 cgcggacgcc gcaccagcgc cacaaagcga cgcgggctcg gccggattcg cggtccagcg
     5521 ggaccaggag gccgggacct cgttggtgtg gcagcgcgtc tcgggcgacg agggtgtcgt
     5581 cgccccggcg gaccggccgg ccgaggacga gccaccgtcg agctcaccgg ccccagcgac
     5641 cccttcgccg cccagcgaac ctgcggttgc gtcggaacct gctgccgagg ccgcagcacc
     5701 gccgacggaa gcacccgcgg ccacccctgc ggccagtacg ccgacagcgt ccggcgccgc
     5761 ggccccgccg agcgcggcgg agctggacga gatggcgaag cgcctgtacg aaccggtgtc
     5821 cgcgcgcctg cgttccgagc tttggctcga cctggaacgc cacgggctcg tgaccgcccg
     5881 gagccgatga gaggaggacg cgttgaccga gagctacccg gaaccggcgg tgaccgtctg
     5941 cttcggcgtc acgatcgacg acaccaggct cggcacgttc aacagctgtg agggcctcgg
     6001 tgtcgaggtc gtgctcgagc agcgcgagga aggcggcaac aacggcttcg tctggcagct
     6061 gccgtcacgt ctgaagttct ccaacatcaa gctgtcccgt ccggtctgca aggacaccac
     6121 cgaggtcatg aactggctgt cgagcatgac gaagaaggtg gagccgcgga ccgcgagcat
     6181 caaggcgatg acggccgagc gaaaggtcgt cgcgacctgg aatctgctcg gggtcatccc
     6241 ggttcggtgg tccgggccgg ccttgaacct ggactccccc aaggtggcga tcgagacgct
     6301 ggagatcgcg catcacggtt tcgccccggc gaaggcgggc gcatgaacca gcccattgcg
     6361 ttgatgacct cgtcatccgg cgccggagtg gccgcacccg cgccgtcggg cgacccgtca
     6421 cggcctcagc tcgagcgcgc atcgctcaag ctgtacgacc cgacgcccgc cggcggcggc
     6481 ggtggagcac cgggcggcca gcgtggcagc atcgctttcc agttcaaccc caaggaagtc
     6541 acgatcgcca agacggcgaa gtgggagcgc aagaccgcca agggcgcgaa gaaggccggt
     6601 ccaccggagt tctccggctc agagccgtgc aagctcacga tggagatgtt cttcgacgcc
     6661 tccgggacac aggacggcag cgtcgtccaa gccgtcgagc agctgttcag ctgctgtgtc
     6721 ccgaccgacg acagcatcgg caagaagaag ccgacccctc cactcgtcgt gctgagctgg
     6781 ggcaaggtca ccagcttccc ggcgttcgtg acctcggtca gtgcgaagta cacgttgttc
     6841 agcgcggagg gcctaccgat ccgggcgctc tgctcggtcg cgctcgagga gatgccgaac
     6901 gagccatggc ggcagaaccc cacgtcggga ggtcggtccg tccggcggtc gcacacgatg
     6961 atcgagggcg acacgctggc ttccgtcgcg tacgccgagt acggcgaccc gacgatgtgg
     7021 cgcgccgtag cggcgtacaa cgggatcgac gacccgatgc gggtagccgc cggccgtgac
     7081 ctgctgctgc cggcacccga ggagctgggg tagccgccat gtcgatcacg aacacgttcc
     7141 tcgtcgatgt cgacgggacc ccgctgccgt ccgacatcac tccgatgctg acggctgcat
     7201 acgtcgacga cagccaacag ctgccggaca tgttcgagct gcggttccgc gacggtcagc
     7261 acgtcgtgct cacgaagacg aacgcgaaga tcggttccac tgtgaggatc tcggtgatga
     7321 ccagcacgag ccagtcgccg gccctgctga tggagggtga gatcacagca ctcgaggcgg
     7381 agttcgacca cggcggcacg ttcacggtga tccgcgggta cgacgccgca catcggctct
     7441 ttcgcggtcg acggaccgag agctacaccc agatgacggc gtccgacatc gcgtccaagg
     7501 tggcgcagcg ggccggtctg aagaccgggg acatctcgtc gacaaccacg gtcttcgagc
     7561 acgtctcgca ggccggcacc agcgactggg agctgctcga ctcccttgcc cggcagaacg
     7621 gatacgagat ctcggtgcgc gagggaaagt tcaacttcgg cccacccacg caggccggca
     7681 gcgcgcccgc cgccggtggc gcgaagccgc agaacccgct cgtgctccaa ctcggtacga
     7741 acctgctccg cttccgttcg gtcgtgacgt cagccgaaca ggtcaaggag gtggaggtgc
     7801 gggggtggga cgtggcgacc aagaaggcgc tcaccaccac ggtgccggcc gccacgaaga
     7861 tggccgagct ccgggacgcg acaccggccg ggctggcgca cacgttcggc gaccccgtct
     7921 acgtgtcgac cgacgtcccg caccgtgcgc aggcagaggt ggacaccgcg gcggcggccc
     7981 tcgtcgagga gatcgccggc ggcttcgccg agttcgaggg cgtcgtgatg ggcaacccgg
     8041 acgtccgcgc cggagcagcc gtgaccgtcg acaacctcgg ttcgccgttc gacggcaagt
     8101 acaccgtcac ccggtcgcgg caccggttcg accctgccac gggctacacg accgcgttct
     8161 ccgtcaccgg ggtccgtgac cgcagcctgc tcgggctgac cggcggggcg ggtcatcctc
     8221 ccagcgcccc cgacggcgtc gtgatcgccc aggtcgacga cgtcaaggat cccgacggcc
     8281 aggctcgggt gaagctcacg ttcccatggc tgtccgacac ctttgtgagc acctgggcgc
     8341 gcacggtcca ggctggggcc ggcaaggatc gcggtgcgac ggtcctgccg gaggtgggcg
     8401 acgaagtgct ggtcgccttc gagcagggcg acttccggcg gccgtacgtc atcggtggtc
     8461 tctacaacgg cgttgacacg ccgagcacga agggcgtcga cctcgtcgac tcgagctcgg
     8521 gcgcggtcaa ccgccggtcg ttggtctcgc gtcagggaca ccggatcgac ctgctcgacc
     8581 aggacgggaa gtccgagggc atcagcatca cgagcaagga cgacaaggtg agtctgctga
     8641 tggactcgac gaacacgaag atcaccgtgc actccgacgg aaccgtgctg atcgaggcga
     8701 ccaacggggt cgtcgtggac tccaagagct cgaagctgga gctgaagggg ggccaggtgt
     8761 ccatcagcgg cagcaacggc gtgacggtcg acggcggttc tggtcagctc cagctgaagg
     8821 gtacgacggc gtcgctcgag ggcagcgcga cgaccgaggt caagggcggc tcgctgtgca
     8881 gcgtctcggc gggcctggtg aagatcaact gagggaggga cgatgcctgc agcagcccgc
     8941 gtcggcgacc cgaccggtca ccccggtgtc atcgggccac cgggtgtgcc caccgtgctc
     9001 atcgccggcc agcccgcggc gactgtcggg accccgcaca tctgctcgtt cccgccaccc
     9061 gcggtccatc cgccgaccgc gatcgtcccg ccaggttgcc cgaccgtgct catcggtggg
     9121 atgccggctg ctcggatggg cgacatggcc ggctgcggtg cgccgatcgt catgggggca
     9181 ccgacggtgc tgatcggagg atgacgtgaa ccacgacttc atcggcgcag gctgggcgtt
     9241 cccggtcggc accgacgcca ccggaagcat cgcgctggtc actcgggagc gtgagatcga
     9301 gcaggcgatc cggctcgtcc tcggcactgc gcgcggcgag cggccgatgc gtccggagtt
     9361 cggttgccgg atccacgagc atgtcttcgg gtccgcgaac gccgcgacag cagggcagat
     9421 cgcctatgac gtccgcgagg ctctcgaacg ctgggagccg cggatcgatg tgctcgaggt
     9481 caacgtgggc ttcgaccaga tcatggtcgg cgtgctgtac gtcgacgtcg gctacgtcat
     9541 cgtcgggtcc aacgacccgc gcaacctcgt gttcccgttc tacgtgatcc ccgagcagga
     9601 ggccccggcc gtcgtacctg cgagcacggc gacgctgcgg gccgtcggat cgggggtgtg
     9661 acatgctccc tgctcccaac ctcgacgacc gaacgttcca gggtctggtg gacgaggcca
     9721 agcgactggt gcaacgacgg tgccccgagt ggaccgacca caacgtctcc gatcccggcg
     9781 tgacgttgat cgaggcgttc gcccagatgg tcgaccagct gatctaccga ctcaaccgcg
     9841 taccggacct gaactacgtg aagttcctcg agctcatcgg cgtcgagctc aggccaccgt
     9901 cggcagctcg cggcgccgtc acgttctggc tctcggcgcc gcaaccgcag cctgtcctcg
     9961 tccgggccga gaccgaggtg gcgacgccac ggaccgacat tcacgagccg gtcgtgttca
    10021 cgacaacgca ggacctggag atcctgccgt gctcgctctc gcgcgcgggc atccacccga
    10081 acggcgccga gccgtccgac ctcaccgagg cgctgtcggg tccgcaaggg ttcgcctgct
    10141 tccaggcgac gccggtgccg ggcgacgcac tgctcatcgg tctgtccgac gcggtgccct
    10201 cgtgcgcggt ggcgatccgc ctcgactgcg cggtggccgg cggtggcggc gtcgatccgc
    10261 gccggccgcc gctgctctgg gaagcgtgga ccggcaccgc ctgggtccgc tgcgacgtcg
    10321 acagcgacga gacagggggc ttgaaccggc ccggtgacgt gatcgtgcat ctcccgccgc
    10381 aacaccagac gtcgatcatc gcccggcacc gagccggctg gctgcgctgc cgggtggtcg
    10441 aggcggagcc cggccagccc acctacacgg cgtcaccacg cattctgtcg gccacggcgt
    10501 tcacgatcgg cgggaccgtg ccgatgatgc acgccgaggt ggttcgacgt gagctggtcg
    10561 gccgctcgga cggaacaccc gggcagcgct tcccgctgca gcggcgcccg gtggtgccgt
    10621 gggaagagcc gacaaccgtc gagatcgtcg acgagacgac cgccgacacg tggcaggccg
    10681 tcgccgactt cgccgcctca ggcccggacg acccgcactt ccggatcgac cactacgccg
    10741 gtgaggtcca gttcggtcct tcggtccggc tgaccgacgg gggcctccgc tcgtacggcg
    10801 ccgtgccggc atccggggcg tccgtcatgg tcacgtcgta ccgtaccggc ggtggccgcc
    10861 gagggaacgt cgctcgcggc cagatccggg tcctcaagac gagcgtgccg tacgtcgccc
    10921 gggtggagaa ccgggcaccg gcgatcggtg gggccgacgc cgagacgctc gacgaggcca
    10981 aggtgcgtgg gccgttgctg ctgcgctcgc gaggtcgggc cgtcaccagt gaggacttcg
    11041 aggaactcgc gcgcgaagtg gcgccggacg cagcacgggt gcattgtgtc gtcaccgagc
    11101 agggtgccga ggacggcggg atcaggttgc tggtggtgcc gcacgtcgcc ggggacgaga
    11161 tcggccggat tcgccggcag gacctgatcc cgccggacgc gacgctggag cggatcacgg
    11221 ccagtctcga cgaacgtcgt ctggtcggga cccggttgct cgtcgccccc ccggagtacg
    11281 tgggactgac cgcggtggtc gacgtcagcg cccgttcgcg attcgacccc gacgaggtcc
    11341 gcgacgatgt gctgcgcgcg gtctaccagc tcttcgaccc actcaccggc ggccccgacg
    11401 gcggcggctg gccgttcggt cgctcggtcc agtcccacga ggttcacgcc gcgctgtcgc
    11461 ggatctccgg tgtcgacatg gccgaggaga tcagcgtgtc gctgttcccg gccgacgcgg
    11521 agaccggacg gcgatcagca gccgtccaac ggctcgacct gccaccgacc gcgctcgtct
    11581 tctcctacga gcaccaggtc cgggtacggc gatgaggggc gcgatcgacg ggctggtgag
    11641 cccgcacccc atcggcgaga cgcttccctc gatgtaccag ggggacccgt tcacccgtga
    11701 cgtctgcgac gcactcgacg acgtgctggc gccgacgctg tgcacgctgg acaacctggc
    11761 ggcgtacctc gacctctcga ccgccccgga ggacctgttg ccgtggctgg caggttggat
    11821 cggcatgagc ctcgaccgcg gtcagcagcc cgagcggcag cgtgagctgc tccgtgcggc
    11881 gggtcgacta caggggtggc agggaacagc tcgtgggatc gagctcgcca tcgaggcggt
    11941 cctcggcatg cgcacggagg tcctcgagtc cggcggcgcc atctggtcgc tcgacccgaa
    12001 tgatcccgtg ccgggtgaac ccgtgccgtc gatcgttgtc cgggtcttcg tcggagacga
    12061 acatcaacac gctccggacg tgggacagca ggtcgacgag ggacgcctca acgccgtgat
    12121 cgaggccatc aagcccgttc acgtccgcca tcgcctgcag gtggtcacca cgactcagcc
    12181 ggatcaaccc gacacctgaa ccagtcccca tcgccgaggt ggcaagtttg gttgggtttt
    12241 tacggcgtgt cgcaccccga t
//
LOCUS       DATBFZ010000160         2663 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_4976495, whole genome shotgun sequence.
ACCESSION   DATBFZ010000160 DATBFZ010000000
VERSION     DATBFZ010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2663
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4976495"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            93..599
                     /gene="paaD"
                     /locus_tag="VH419_04820"
     CDS             93..599
                     /gene="paaD"
                     /locus_tag="VH419_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012920594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaD"
                     /protein_id="HEY7042972.1"
                     /translation="MTSRSSTTAQPSATAARAIVAEVLDPEVPVLTIEDLGILREVTV
                     DEAGHVDVQITPTYSGCPAMDAIRADVTRALASNGFDDVDVHLVLAPAWTTDWMSEDG
                     KQKLLDYGIAPPQPRGERPVPVALSLRCPQCGSGDTRELSRFGSTACKSLWVCNTCQE
                     PFDHFKTH"
     gene            607..1689
                     /gene="paaE"
                     /locus_tag="VH419_04825"
     CDS             607..1689
                     /gene="paaE"
                     /locus_tag="VH419_04825"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020575595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaE"
                     /protein_id="HEY7042973.1"
                     /translation="MAPAPHKAVFHTLRVGDVTPLTDDSVCISFEVPTQLRDDYDFVQ
                     GQHLTIRTPKAGDDVRRSYSICAPAGHGKLRIGVKVLPGGHFSGFAAGKLKVGDELDV
                     MTPTGRFYTPLDPANAKHYCAIAAGSGITPVLSLIATTLEQEPDSRFTLIYANRTSRT
                     VMFLEELEDLKDVHRGRFHLLHVLSREEQDAELLSGRLDGPRLTKITETLVPAETVDE
                     WFLCGPYAMVLDLREALLEQGANAAHVHTELFHVETKPPERRTTQADEGEGADVSIVL
                     DGRRSSFKLKGNDVSILEAALRVRSDAPFACKGGVCGTCRAKLVDGTVAMDTNYALEP
                     DELDKGYVLTCQSHPTSTSVALDYDA"
     gene            1843..2637
                     /locus_tag="VH419_04830"
     CDS             1843..2637
                     /locus_tag="VH419_04830"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEY7042974.1"
                     /translation="MRQYLDLMQRVLDEGVEKSDRTGTGTLSVFGHQMRFGLGRGFPL
                     VTTKKIHTKSVFGELIWFLRGDTNVGWLHERGISIWDEWADEHGDLGPVYGYQWRSWP
                     TPDGQYVDQIAHVVDQIRSNPDSRRHIVSAWNVADIPRMALAPCHALFQFHVADGRLS
                     CQLYQRSADVFLGVPFNIASYALLTHMVAQVTGLEVGDFVHTFGDAHLYRNHLEQART
                     QLARAPRELPELWLDPSVTELADFDLEHIKVSGYDPHPAIRAPIAV"
BASE COUNT          460 a          885 c          900 g          418 t
ORIGIN      
        1 cgaacggctg gtgacccagg caaacctgcc ttgggagggg gcggtctgcc tggtcaccga
       61 ggcaaccgcc caacgcggcg gagggcgaca gcatgacatc gcgatcgagt acgacggcgc
      121 agccgtccgc caccgcggcg cgggcgatcg tggcggaggt gctcgacccg gaggtccccg
      181 tcctcacgat cgaggacctc ggcatcctcc gagaggtgac cgtcgacgag gcggggcatg
      241 tcgacgtaca gatcacgccg acgtactccg gttgtccggc catggacgcg atcagggccg
      301 acgtcacacg agcccttgcc agcaacgggt tcgacgacgt cgatgtccac ctggtgcttg
      361 caccggcttg gacaactgac tggatgagtg aggacggcaa gcagaagctg ctcgactacg
      421 gcatcgcccc cccgcagccg cgcggcgaac gaccggtgcc ggtcgcgttg tcgctgcgct
      481 gcccgcagtg cgggtcgggt gacacgcgag agctgagccg gttcgggtcg acggcgtgca
      541 agtccttgtg ggtgtgcaac acctgccagg agcccttcga ccacttcaag acccactagg
      601 acccggatgg ccccggcacc gcacaaggcg gtcttccaca cgttgcgcgt cggcgacgtg
      661 acgccgctga ccgacgactc cgtctgcatc agcttcgagg tgccgacgca gctgcgcgac
      721 gactacgact tcgtgcaagg acagcacctc acgatccgga cccccaaggc cggtgacgac
      781 gtacgccgca gttactccat ctgtgctccg gcgggtcacg gcaagctgcg catcggcgtg
      841 aaggtgctgc cgggcggcca cttctccggg ttcgcggccg gcaagctgaa ggtgggcgac
      901 gagctggacg tgatgacgcc caccggccgg ttctacacac cgctcgaccc ggccaacgcc
      961 aagcactact gcgcgatcgc ggccggcagc gggatcacgc cggtgttgtc gctgatcgcc
     1021 accacgttgg agcaggagcc ggacagccgt ttcacgctga tctatgccaa ccggacgagc
     1081 cggacggtga tgttcctcga ggagctcgag gacctgaagg acgtccatcg cggccggttc
     1141 cacctcctgc acgtgctgag tcgggaagag caggatgccg agctgctgtc gggacgactc
     1201 gacgggccac ggctgaccaa gatcaccgag acgttggtgc cggcggagac ggtggacgag
     1261 tggttcctgt gtgggccgta cgcgatggtg ctcgacctgc gtgaggcgct gctcgaacag
     1321 ggcgccaacg ctgcacacgt tcacaccgag ctgtttcacg tcgagaccaa gccacccgag
     1381 cggcgtacga cccaagccga cgaaggtgag ggcgccgacg tctcgatcgt gctcgacggt
     1441 cggcgctcgt cgttcaagct caagggcaac gacgtgtcga tcctggaagc ggccttgcgg
     1501 gtgcggtcgg acgcgccgtt cgcctgcaag ggcggtgtct gcgggacgtg ccgggcgaag
     1561 ctcgtcgacg gcacggtggc catggacacc aactacgcgc tggagcccga cgagctcgac
     1621 aagggctacg tcctgacctg ccagagccac cccacgagta cgtcggtggc gctcgactac
     1681 gacgcctgag tggctgcacc tggaaggttt gcgatgttta cagcccacct ggcctgtgaa
     1741 catcgcagat ccgcgctgca cagtggaggc ttgcgatgtc tacctcgcac gcctcagcgt
     1801 gtctaggaag ggccgtctgg ggagcggact aggctggccc gggtgcggca gtacctcgac
     1861 ctcatgcaac gcgtgctcga cgaaggcgtc gagaagagcg accgcaccgg caccggcacg
     1921 ctgagtgtct tcggtcacca gatgcggttc ggccttggcc gcggcttccc gctcgtgacg
     1981 acgaagaaga tccacacgaa gtcggtattc ggcgagctga tctggttcct gcgtggcgac
     2041 acgaacgtcg ggtggctcca cgagcgcggc atctcgatct gggacgaatg ggccgacgag
     2101 cacggcgacc ttggcccggt ctacggctac cagtggcgct catggccgac gccggacggg
     2161 cagtacgtcg accagatcgc gcacgtcgtc gaccagatca ggtccaatcc cgactcgcgc
     2221 cggcacatcg tcagtgcctg gaacgtcgcg gacatcccga ggatggcact cgcgccatgc
     2281 cacgcgctgt tccagttcca cgtggccgac gggcggttgt cgtgccagct ctaccagcga
     2341 tcggctgacg tgttcctcgg cgtaccgttc aacatcgcgt catacgccct gctgacgcac
     2401 atggtcgctc aggtcactgg tctggaggtc ggcgacttcg tccacacgtt cggcgacgcc
     2461 cacctctacc ggaaccactt ggagcaggcg cgcacgcagc tcgcccgggc gccacgcgag
     2521 ctgccggagc tgtggctgga cccgtcggtg accgagctgg cggacttcga cctcgagcac
     2581 atcaaggtgt ccggatacga cccgcatccc gccatcaggg cgccgatcgc cgtctgaggc
     2641 gcttgcggcc acggcgccgg taa
//
LOCUS       DATBFZ010000161         4288 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_3662594, whole genome shotgun sequence.
ACCESSION   DATBFZ010000161 DATBFZ010000000
VERSION     DATBFZ010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 4288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4288
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3662594"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /locus_tag="VH419_04835"
     CDS             complement(<1..430)
                     /locus_tag="VH419_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042975.1"
                     /translation="MPGIDELERGLQPPEDLALGGPDLAAIRGEGRRLRRRRQLLSAG
                     VAAAVTAGVATAALAVPQALDHGDEQTQAAGTVTAPPTKDSDLLERCRNGENSPHAVA
                     LLFDPGTPTVEAVSRSPHGVSLAIESADARYWAECHIALKR"
     gene            complement(432..941)
                     /locus_tag="VH419_04840"
     CDS             complement(432..941)
                     /locus_tag="VH419_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009085840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigE family RNA polymerase sigma factor"
                     /protein_id="HEY7042976.1"
                     /translation="MSAPAAERQFEEFVTAAWPRLRWTAYLLCGDHHLAEDLTQTALA
                     RTYSAWRRIRRDDPTAYARAVLVNLNIDRMRRRRFLEVSEEQAPESVSEPAAGDLERR
                     EQVVALLRQLGDRERAVVVLRHYYDLSEAQVAAELGIPRGTVKSTLSRALSKMRVTTE
                     SVGSMNGRD"
     gene            complement(1027..2631)
                     /locus_tag="VH419_04845"
     CDS             complement(1027..2631)
                     /locus_tag="VH419_04845"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HEY7042977.1"
                     /translation="MPYPSTRTSDDIVAPTLADPMARAATTRVGGPVGRHNLAGAGWW
                     TPLRITLVVATVCFGLGIVQKSPCVVTDFGDLAKPKAFSHMCYSDIPYLYSGRGLAEG
                     ILPFTPSDDLPQTVQVTAQDPHPVSIEYPVLTGVWMGLTGAITHVIGKDPDLSQVPHQ
                     NVGYNLDVQYDSAIFWSVNAVGFFVVLMLAMALLVAAQRRRPWDAVYVAASPVLALTA
                     MINWDILAIGFVAGVLWAWSTRRPVVAGIFIGLGTATKLYPLFLLGPLLVLCLRERKM
                     DVWVKAGAAAIASWLIVDLPVYFWSPDEFLWFWQFNASRGPDFGSVWGAFSEYGHAAT
                     PHTINVVTWIFFGGCCLAIAALGLLAGRRPRVPQLALLVTVAFLLVNKVYSPQYVLWL
                     LPLAALARPRWRDLLIWQTCEVLYFFAVWMHIAEFFVTPGDPDWAYVTAIVIRVLGEL
                     YLCAVVIRDILSPWHDPVRMDGLSDDPLGGVLDEGIDSEPMQHADEDSWEHDSHHHDH
                     THHEAAGEHRGDADHEHPERDPIYAP"
     gene            complement(2726..>4288)
                     /locus_tag="VH419_04850"
     CDS             complement(2726..>4288)
                     /locus_tag="VH419_04850"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEY7042978.1"
                     /translation="PGTLDPAQGKAAKRELKERYQYVLAGMVSMGNLDADEAAKYEKR
                     LPEFPEIPEVSNYGGQRGYLLNLVEQKLQQLKFTDEEINGGGLKVVTTLSWQDSIAAA
                     KAVTEERPEGRPQLHVALSAVDPRTGALRAMIGGRDYTGGGPKDQVNFALAPTQVGST
                     MKAYAVAAGLKDGFTLRDTFDGNSPYTYPNGETVRNEGQSSGNPTGNSYGAAIPLTYA
                     TQESVNTAFADLTSRMKNGPQKVVDAAVAAGVPKESPDLEPNLGVALGSATIPNVEMA
                     EGYSTFADNGVHHDWYVIEKVTDANGLRYEHKIQGDRAFTPAISSNVTYALEQVVKDG
                     TGAAAQALERPAAGKTGTATFGTADNQYVSSSWFVGFTPQLSTAITYTRGDGNDSLEG
                     YLEPFFGANYPTQTWTSFMEKALAGSPELDFPEPAELHGENPTYSPPPTTSEPPTTTS
                     EPPTTTSEPPTTTSEPPTTTSEPPTTTSEPPTTPSEPTDTCPILGCPTTSTTTTTTDE
                     EDPDPPGAGPDG"
BASE COUNT          654 a         1452 c         1482 g          700 t
ORIGIN      
        1 cccgcttcaa cgcgatgtgg cactcggccc agtaccgggc gtcggcggac tcgatggcca
       61 gcgacacgcc gtgaggagag cgggacaccg cctcgacggt gggagtgccc ggatcgaaga
      121 gaagcgcgac cgcatgcggg ctgttctcgc cgttgcggca cctctcgagc aggtcggagt
      181 ccttggtggg tggtgccgtg accgtcccgg cggcctgcgt ctgctcatca ccatggtcca
      241 gtgcctgcgg cacagcgagc gccgccgtgg cgacaccggc ggtgacggcc gctgcgacac
      301 ctgccgacaa cagctgccgc cgtcgccgga gccgacgccc ctcaccgcgg atcgcagcca
      361 ggtccggtcc gccgagagcc aggtcctcgg gcggctgcag gccgcgctcg agctcatcga
      421 tgccgggcat gtcagtccct tccgttcatg gatccgacgg actccgtggt gacccgcatc
      481 ttgctgagcg cgcgcgagag cgtgctcttc acggtcccgc gcgggatgcc cagctcggcc
      541 gccacctggg cctccgagag gtcgtagtag tgccgcagca ccacgacggc gcgctcccgg
      601 tcgccgagct gtcggagcag cgcgaccacc tgctcccgcc gctcgaggtc cccggctgcg
      661 ggctcgctga ccgactccgg tgcctgctcc tcggacacct ccaggaagcg gcgccgccgc
      721 atccggtcga tgttgaggtt cacgagtacc gcacgtgcgt atgccgtggg gtcgtcacgc
      781 cggatcctgc gccacgcgga gtaggtccgc gcgagtgccg tctgggtgag gtcctcggcc
      841 aggtggtggt cgccacacag caggtatgcc gtccagcgca gccgcggcca ggcggccgtc
      901 acgaactcct cgaactgcct ctccgcggcc ggcgcgctca tctgtcctcc gtcgtcgagg
      961 ggcccttccc ctcacccctc aagacgggtc ctggccgaga tcggttctct ccccgtcgcg
     1021 acgcggtcat ggcgcgtaga tcgggtcccg ctccggatgc tcgtggtcgg catcgccgcg
     1081 gtgctcaccg gcggcttcgt gatgggtgtg gtcgtggtga tgactgtcgt gctcccacga
     1141 gtcctcgtcg gcatgctgca tgggctctga gtcgatgcct tcgtcgagca cgccaccgag
     1201 cgggtcgtcg gagagaccgt ccatccggac ggggtcgtgc cacggcgaga ggatgtcgcg
     1261 gatgaccacc gcacacaggt acagctcgcc gaggacccgg atcacgatcg ccgtgacgta
     1321 ggcccagtcg gggtcgcccg gcgtgacgaa gaactccgcg atgtgcatcc agaccgcgaa
     1381 gaagtacagg acctcgcagg tctgccagat caacaggtcg cgccagcgcg gccgagccag
     1441 ggcggccagc ggcaacagcc agagcacgta ctgcggcgag tacaccttgt tgacgagcag
     1501 gaacgccacg gtgacgagca gcgccagctg aggaacgcgc ggacgccggc ccgcgagcag
     1561 gccgagtgcg gcgatcgcca ggcagcagcc gccgaagaag atccaggtga ccacattgat
     1621 cgtgtgcggc gtcgcggcat gcccgtactc cgaaaaggca ccccagaccg agccgaaatc
     1681 cggaccccgt gaggcgttga actgccagaa ccacaagaac tcgtccggcg accagaagta
     1741 caccggcagg tccacgatga gccacgaagc gatcgcggcc gcacccgcct tcacccagac
     1801 gtccatcttc cgctcgcgca ggcacagcac cagcagcggg ccgagcagga acagcggata
     1861 gagcttggtc gccgtcccca gtccgatgaa gatgccggcg accaccggtc ttcgggtgga
     1921 ccaggcccac agcacgccgg ccacgaaccc gatggcgagg atatcccagt tgatcatcgc
     1981 ggtcagtgcc agcaccggtg acgccgcgac gtagacggcg tcccatggcc tccgacgttg
     2041 tgccgcgacg agcagcgcca tcgcgagcat cagcacgacg aagaagccca ctgcgttgac
     2101 ggaccagaag atcgccgagt cgtactggac gtcgaggttg tagccgacgt tctggtgcgg
     2161 cacctgagac aggtcaggat ccttgccgat cacatgcgtg atggctccgg tgagccccat
     2221 ccacaccccg gtgagcaccg ggtactcgat cgacaccgga tgtggatcct gagcggtgac
     2281 ctgcacggtc tgcggcaggt cgtccgacgg cgtgaacggc aagatgccct cggcgagacc
     2341 ccgaccggag tacaggtacg ggatgtcgga gtagcacatg tgcgagaacg ccttgggctt
     2401 ggcgaggtcg ccgaagtccg tgaccacgca cggagacttc tgcacgatgc cgagcccgaa
     2461 gcacacggtg gcgacgacca gggtgatgcg caacggcgtc caccacccgg cgcctgcgag
     2521 gttgtgcctc ccgaccggac cgcccacccg cgtcgtcgcc gcacgcgcca tcgggtcggc
     2581 aagcgtcggc gcgacgatgt cgtcgctcgt gcgggtcgag gggtacggca cgcagccatg
     2641 gtgcctcatc gccctcaccc gcacgcagca gaccggctag cgcttgtctc ccaccactcg
     2701 atcatcgcgc gtggggagac acgtgctacc cgtcgggacc cgcaccgggc gggtcaggat
     2761 cttcttcgtc cgtcgtcgtc gtggtggtgc tggtcgtcgg acaccccaga atcgggcagg
     2821 tgtcggtcgg ctccgatggc gtcgtcggtg gctccgacgt cgtcgtcggt ggctcggagg
     2881 tggtggtcgg tggctccgac gtcgtcgtcg gtggctcaga ggtggtggtc ggtggctccg
     2941 acgtcgtcgt cggtggctcc gaggtggtcg ggggcggcga gtacgtcggg ttctccccgt
     3001 gcagctcggc cggctcgggg aagtccagct ccggcgagcc ggccagcgcc ttctccatga
     3061 acgacgtcca cgtctgggtc gggtagttgg cgccgaagaa cggctcgagg taaccctcga
     3121 gagagtcgtt gccgtcaccg cgggtgtacg tgatcgccgt cgacagctgt ggagtgaagc
     3181 cgacgaacca ggacgacgag acgtactggt tgtccgccgt gccgaacgtc gcggtgcccg
     3241 tcttgccggc ggccggccgc tcgagggcct gggccgccgc gccggtgccg tccttgacga
     3301 cctgctccag cgcgtacgtc acgttgctcg agatcgcggg cgtgaaggca cggtcgccct
     3361 ggatcttgtg ctcgtagcgc aggccgttgg cgtctgtgac cttctcgatg acgtaccagt
     3421 cgtggtgcac gccgttgtcg gcgaacgtgc tgtagccctc ggccatctcg acgttgggga
     3481 tggtggccga cccaagagcg acaccgaggt tcggctcgag gtcgggcgac tccttcggga
     3541 caccggccgc caccgcggcg tcgacgacct tctgcgggcc gttcttcatc cgggaggtga
     3601 ggtcggcgaa cgcggtgttg accgactcct gcgtcgcgta ggtcaacggg atcgccgcgc
     3661 cgtacgagtt gccggtcggg ttgcccgacg actggccttc gttgcggact gtctcgccgt
     3721 tcgggtacgt gtacggcgag ttcccgtcga acgtgtcccg caacgtgaag ccgtccttga
     3781 ggcccgccgc gacggcgtac gccttcatcg tcgagccgac ctgggtcggg gcgagggcga
     3841 agttcacctg gtccttggga ccgcctccgg tgtagtcgcg tccgccgatc atggctcgta
     3901 aggcacccgt acgcgggtcg accgcggaca aggcgacgtg cagctgcggg cgaccctcgg
     3961 gacgctcctc cgtgaccgcc tttgccgccg cgatcgagtc ctgccagctg agtgtcgtga
     4021 cgaccttgag gccaccgccg ttgatctcct cgtcggtgaa cttgagctgt tgcagcttct
     4081 gctcgacgag gttgagcagg tacccgcgct ggccgccgta gttgctcacc tcggggatct
     4141 cgggaaactc cggaagccgc ttctcgtact tggcggcctc gtcggcatcg aggttgccca
     4201 tcgacaccat gcctgcgaga acgtactggt agcgctcctt gagctcacgc ttggccgcct
     4261 tgccctgggc cgggtccagc gtgccggg
//
LOCUS       DATBFZ010000162         1834 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_105348, whole genome shotgun sequence.
ACCESSION   DATBFZ010000162 DATBFZ010000000
VERSION     DATBFZ010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 1834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1834
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105348"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="VH419_04855"
     CDS             <1..332
                     /locus_tag="VH419_04855"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEY7042979.1"
                     /translation="PVVVVTHRPPPDDSADRFPRTTFTGSVAEGIATAKEIARDKFVT
                     IASASIIQQALNLGLVDELCLSQVPVLFGTGIRYFGELVGDHVMLEDPAVVQGTRALH
                     LRYPVRR"
     gene            complement(392..1141)
                     /locus_tag="VH419_04860"
     CDS             complement(392..1141)
                     /locus_tag="VH419_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015068790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY7042980.1"
                     /translation="MTKRSLRHTTRSVDTIITVVLTPIAILLLFVYVFGGALGKQTGS
                     IDYVDYITPGVVIMTVASGIAYAALRLSTDLQQGIISRFRTMPVTPSSVLNGQVLSST
                     LSNLFSCFLVILVALLVGFRSGADPAAWLAFFGLLVVVILATTWLAMLFGLLAKTPEG
                     AGAFSYVLLLLIFISPSFVPTDSMTPLLRGFANNQPMTPIIESMRSLLTNGTPGSDIW
                     AAVAWCTGILLVSFTLALGVYRRSAPVPATQ"
     gene            complement(1152..>1834)
                     /locus_tag="VH419_04865"
     CDS             complement(1152..>1834)
                     /locus_tag="VH419_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017936642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY7042981.1"
                     /translation="DHLLSARENLVLMAELRHVADPRQTAADLLDTFDLAEAADRRLL
                     TFSGGMRRRLDIAMSLVGDAPIIFFDEPTTGLDPKSRTDMWTTIRRMVEKGTTVFLTT
                     QYLDEADRLADEIAILHGGRIVADGTPDELKAMVPTGVVELEFSEEAQLSAAQRALGE
                     QHEVNRADSKLVVATTGSVAEMADIFMRLKDRGIEPTEFSRPRPTLDDVFFKILDDEA
                     EGLHASVQ"
BASE COUNT          304 a          593 c          641 g          296 t
ORIGIN      
        1 caccggtcgt cgtcgtcacc caccggcccc cgcccgacga ttcggccgat cggtttccgc
       61 ggacgacctt cacgggcagc gtcgccgagg ggattgcgac ggcgaaggag atcgcccgtg
      121 acaagttcgt cacgatcgcg agcgccagca tcatccagca ggccctgaac ctcggcctcg
      181 tcgacgagct ctgcctcagc caggtgccgg tgctgttcgg caccggcatc cgctacttcg
      241 gcgagctcgt cggcgaccac gtcatgctcg aagaccccgc cgtcgtccaa ggcacccggg
      301 cgctgcacct ccgctacccg gtacggcgct gaagcttgcc tccacacagc ctcgacctgt
      361 cctggtcggc agcccacgga ccgccggcgg gtcactgcgt tgccgggacc ggtgcgctac
      421 ggcgatagac gcccaacgcg agcgtgaagg agacgagcag gatgcccgtg caccatgcga
      481 cggcggccca gatgtcggaa cccggggtgc cgttcgtcag cagcgagcgc atgctttcga
      541 tgatcggtgt catcggttgg ttgttggcga agcctctcag caacggcgtc atggagtcgg
      601 tcgggacgaa cgacgggctg atgaagatca gcagcagcag gacgtagctg aaggcgccgg
      661 caccttcggg ggtcttcgcc agcaggccga acaacatggc cagccacgtc gtggccagga
      721 tgacgacgac caggaggccg aagaaggcga gccaggcggc tggatccgcc ccggagcgga
      781 agccgaccag gagcgccacc aggatcacca ggaagcagga gaagaggttg gacagcgtcg
      841 atgagaggac ctgcccgttg aggaccgacg agggcgtcac cggcatggtc cggaagcggc
      901 tgatgatgcc ttgctggagg tccgtgctga ggcgcagggc ggcgtaggcg atgccgctcg
      961 ccaccgtcat gatgacgacc ccgggggtga tgtagtcgac gtagtcgatc gagcccgtct
     1021 gcttcccgag cgcgccgccg aacacgtaga cgaacaacag caggatggcg atcggtgtca
     1081 agacgaccgt gatgatggtg tcgacgctgc gtgtcgtgtg gcgcaggctc cgcttggtca
     1141 tggtccaggt gtcactgaac gctcgcatgc aggccctccg cttcgtcgtc gaggatcttg
     1201 aagaacacgt cgtccagggt cggccgcggc cgggagaact ccgtcggttc gatcccgcgg
     1261 tccttcagcc gcatgaagat gtcggccatc tcggcgaccg agccggtggt ggccacgacc
     1321 agcttcgagt cggcccggtt cacctcgtgc tgctcgccga gtgctcgttg ggcggccgac
     1381 agttgcgctt cttcgctgaa ctccagctcg acgaccccgg tgggcaccat ggccttcagc
     1441 tcgtccggtg tgccgtcggc cacgatcctg ccgccgtgga ggatggcgat ctcatcggcc
     1501 agacggtcgg cctcgtccag gtactgcgtg gtcaggaaga ccgtggtgcc cttctccacc
     1561 atgcgtcgga tggtcgtcca catgtcggtg cggctcttgg ggtcgagccc ggtggtcggc
     1621 tcgtcgaaga agatgatggg ggcgtcgccc acgaggctca tggcgatgtc gagccggcgt
     1681 cgcatcccgc ccgagaacgt cagcaggcgg cggtcggcgg cctcggcgag gtcgaacgtg
     1741 tcgagcaggt ccgcggccgt ctgccgggga tcggcgacgt ggcgcaactc ggccatcagc
     1801 accaggttct cccgggcgct gagcaggtgg tcca
//
LOCUS       DATBFZ010000163         3425 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_2401982, whole genome shotgun sequence.
ACCESSION   DATBFZ010000163 DATBFZ010000000
VERSION     DATBFZ010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 3425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3425
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2401982"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..891)
                     /locus_tag="VH419_04870"
     CDS             complement(<1..891)
                     /locus_tag="VH419_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY7042982.1"
                     /translation="MTATAISDAVLIRGIGKRFPGVVANHDVDITIRTGTVHALIGEN
                     GAGKSTLMKILYGVQKPDEGTVTVNGEQVSFHSPTEAIAHGIGMVFQHFMLADNLTVL
                     ENVVLGAERLHGIGTGARDMVRDISRKYGFGLEPDELVERLGVGDRQRVEILKVLYRG
                     AKIVILDEPTAVLVPQEVDALFANLRDLKAQGHTILFISHKLDEVLDIADDITVMRRG
                     TTVGEAKPGSVTKRQLAEMMVGSELPSPETEESTVADEVMLSLVDVTLTDEYGRNRLT
                     NVTFEIHRGEVLGLAGVEGNG"
     gene            complement(1040..2131)
                     /locus_tag="VH419_04875"
     CDS             complement(1040..2131)
                     /locus_tag="VH419_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007078487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY7042983.1"
                     /translation="MRRSTKIAVVLAAATLALAGCAKDDNKSSSGGSTTVLNCDDLTG
                     DGPKVGIAYDVGGQGDHSFNDSAAAGMKKANEDLGATCTEGEAQTGEPESAREDRLRQ
                     MADSGYNPVIGIGFSYSEAADNVAPDYPNVNFGVVDGFDPTPNKDGSPNNDNVAYLDF
                     AANEGSFLVGVAAADTTKSDHVGFVGGVHVDLIKNFEAGYVAGAKAVNPNIKVDVKYI
                     EETDLSGFNDPAGGKAAASAMYDNGADVVFQVAGGSGSGVFDAAVDAGEGNWAIGVDG
                     DQYGSAAADQKPHILTSMIKHVDVATFDMIQSIKDGKPLVGYQTYDLKVGGVGYSTSG
                     DFMSQDTIDNIDSYADKIKSGQIKVPTTP"
     gene            complement(2275..>3425)
                     /locus_tag="VH419_04880"
     CDS             complement(2275..>3425)
                     /locus_tag="VH419_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008363990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEY7042984.1"
                     /translation="EAELIEIRRDLHMHPELGHQETRTTQRIVDRLAAAGLEPRVLSS
                     GTGVICDVAGERGEPPVIGFRGDIDALPLVDVKHVPYRSTVEGVCHACGHDAHVAILL
                     GTGLVLAELAAQGALTRSVRLIFQPAEEVTPGGALDVLADGETNGLERVFAFHCDPKL
                     EVGKLGFRAGSITASADRIRVTMSGPGGHTARPHLTSDLVHALGAVVTGLPTVLSRLA
                     DPRAGLSVVWGQVHAGSAANAIPQEGFAEGTIRCLDAGVWAAAHDRVPDLVRQLAIPF
                     GVDVDVDVHTSVPPCVNDEAATAFIRSVATDVFEADCVTSTDQSLGGEDFGWILGRVP
                     GALVRLGVRSPEVARAGDLHQGSFDIDEAAIGVGVRLFAAAATTPFPA"
BASE COUNT          533 a         1156 c         1125 g          611 t
ORIGIN      
        1 cccattgccc tcgactccgg cgagaccgag cacctcgccc cgatggatct cgaacgtgac
       61 gttcgtcagc cggttacggc cgtactcgtc ggtcagggtg acgtcgacga gcgacagcat
      121 cacctcatcg gcgaccgtgg actcctcggt ctcgggcgac ggcagttcgg agccgaccat
      181 catctccgcg agctgccgct tggtcacgct gccgggcttc gcctccccga ctgtcgtccc
      241 gcggcgcatc accgtgatgt cgtcggcgat gtcgaggacc tcgtcgagct tgtgcgagat
      301 gaacaggatc gtgtgcccct gtgccttgag gtcgcgcagg ttggcgaaca gcgcgtcgac
      361 ctcctgcggc accagcacgg cagtcggctc gtcgaggatg acgatcttgg cgccccgata
      421 gagcactttg aggatctcga cccgttgacg atcccccaca ccgagccgct cgaccagctc
      481 gtctggctcg agaccgaacc cgtacttgcg tgagatgtca cggaccatgt cacgggcacc
      541 ggtaccgatt ccatgcagcc tctccgcccc gaggacgacg ttctcgagca cggtcaggtt
      601 gtcggcgagc atgaagtgct gaaacaccat cccgatgccg tgcgcgatcg cttcggtcgg
      661 agaatggaac gagacctgct caccgttcac cgtgacggtg ccctcgtcag gcttctggac
      721 gccgtacagg atcttcatca gcgtcgactt gccggcgccg ttctcaccga tcagcgcatg
      781 aacggtcccc gtgcggatcg tgatgtcgac gtcatggttg gcgacgacgc cgggaaacct
      841 cttcccgatc ccccggatca gtacggcgtc gctgatcgcc gtggcggtca tggactctcc
      901 cctccctggg ggccgatgac ggtgacgggc ggtgggacga gcgccgggcc ggatctggca
      961 tgacacaggc ccgggacgaa cgctaccgcg aagccccggg cctgtgtgtc accggacggg
     1021 ccggtgtcgt gctgtgccgt tacggcgtgg tcggaacctt gatctgacca ctcttgatct
     1081 tgtcggcgta cgagtcgatg ttgtcgatcg tgtcctggct catgaagtca ccggaggtgg
     1141 agtagccgac gccgccgacc ttgaggtcgt acgtctggta gccgacgagc ggcttgccgt
     1201 ccttgatcga ctggatcatg tcgaacgttg ccacgtcgac gtgcttgatc atcgaggtga
     1261 ggatgtgcgg cttctggtca gcggccgcgg aaccgtactg gtcgccgtcg acaccgatcg
     1321 cccagttgcc ctctcctgcg tcgaccgccg cgtcgaacac tcctgaaccg gagccgccgg
     1381 ccacctggaa caccacgtcg gcaccgttgt cgtacatggc cgaggctgct gccttgccac
     1441 cggccgggtc gttgaatccg ctgaggtcgg tctcctcgat gtatttcaca tcgaccttga
     1501 tgttcgggtt gacggccttg gcgccggcca cgtagcccgc ctcgaagttc ttgatcaggt
     1561 caacgtgaac gccgccgacg aagccgacgt ggtcgctctt cgtcgtgtcg gcggccgcga
     1621 ccccgacgag gaatgatccc tcgttcgctg cgaagtccag gtacgcgacg ttgtcgttgt
     1681 tggggctgcc gtccttgttc ggcgtcgggt cgaatccgtc gacgacgccg aagttcacgt
     1741 tcgggtagtc gggtgcgaca ttgtcggcgg cttcgctgta ggagaaaccg attccgatga
     1801 ccgggttgta gccgctgtca gccatctgcc gaaggcggtc ctcgcgagcc gactcgggct
     1861 caccggtctg cgcctccccc tcagtgcagg tcgcgccaag gtcttcattc gccttcttca
     1921 tgccggccgc cgccgagtca ttgaaggagt ggtcaccctg accgcccacg tcgtaggcga
     1981 tacccacctt ggggccgtca ccagtcaggt cgtcacagtt gaggaccgtg gtcgatcctc
     2041 cgctgcttga tttgttgtcg tccttggcgc agccggccaa ggcgagagtg gcggccgcga
     2101 ggaccaccgc gatcttggtc gaacgacgca agacgactcc ttccacccgg cgtgctcggt
     2161 ggttcggcac gcatccgcgg cagatcataa accaggcaca gaatctgatg ggtatcggtc
     2221 acgggccgac ccggccgtcg atacctgttt gtgacctatt gcaccgttgc ccggctatgc
     2281 ggggaacggc gtcgttgccg cagccgcgaa cagccggacg ccgacaccga tggctgcctc
     2341 gtcgatgtcg aagctgccct ggtggaggtc gccggcccgg gcgacctccg gactgcgtac
     2401 gccgaggcgc accagcgcac cggggacgcg tccgaggatc cacccgaagt cctccccgcc
     2461 caggctctgg tcggtgctgg tgacacagtc ggcctcgaag acgtcggtcg ccaccgaacg
     2521 gatgaaggct gtggccgcct cgtcgttgac gcagggcggg acgctcgtgt gcacgtccac
     2581 gtccacatcg actccgaacg ggatggcgag ctggcgcacc aggtccggca cgcggtcgtg
     2641 ggcggccgcc cacaccccgg cgtcgagaca gcggatcgta ccctcggcga agccctcctg
     2701 cgggatcgcg ttggcggccg acccggcgtg cacctggccc cacacaacgc tcagcccagc
     2761 ccgtgggtcc gcgagtcgcg acagcacggt cggcaggccg gtcaccaccg ctccgagcgc
     2821 gtggaccagg tccgaggtca ggtggggccg cgcggtgtga cctcccgggc ccgacatcgt
     2881 cacccggatg cgatccgcac tggcggtgat ggaaccggca cggaagccga gcttcccgac
     2941 ctccagcttg gggtcgcaat ggaacgcaaa gacgcgttcg agcccgttcg tctcaccatc
     3001 ggcgagtacg tccagcgcac ctcctggcgt gacctcttcc gcgggctgga agatcagccg
     3061 taccgagcgc gtcagcgccc cctgggcggc cagctcggcc agcacgagtc cggtcccgag
     3121 gaggatcgcg acgtgcgcgt cgtgcccgca cgcgtgacag acaccttcga ccgttgaccg
     3181 gtacgggacg tgcttcacgt cgacgagcgg gagcgcgtcg atgtcgccac ggaacccgat
     3241 gaccggcggc tcaccccgct cgccggcgac gtcacagatg acgccggtgc ccgacgacag
     3301 gacgcgcggt tcgagcccgg cggcggcgag gcggtcgacg atgcgctggg tcgtacgagt
     3361 ctcctggtga ccgagctccg gatgcatgtg gaggtcccgg cggatctcga tcagctcggc
     3421 ttcga
//
LOCUS       DATBFZ010000164         2135 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_764207, whole genome shotgun sequence.
ACCESSION   DATBFZ010000164 DATBFZ010000000
VERSION     DATBFZ010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_764207"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1734)
                     /locus_tag="VH419_04885"
     CDS             complement(<1..1734)
                     /locus_tag="VH419_04885"
                     /inference="COORDINATES: protein motif:HMM:NF015684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HEY7042985.1"
                     /translation="MFSIFDRYVVRDAVLPLLLSLALLTFLLIIPPVLQVGYQFLAKG
                     MQLPIVLRVLVTLLPQALSISIPIATLLGLLIAFGRLSADREFVAMQACGVSIYRLLR
                     PVGIIALAASIGTAYVTIVALPNANQSFREITLGVVKSQVQNEVKPRDFFTRFPNTVL
                     YVRNVLAGGLWQDVFFADSHTPGQTMVYFAREGRIVVDRNEKFVQLQLLKGTHHLTRA
                     SDPDVYDGAQFESTSVTLDAEEVFPKPPSRNAPEMTIAELRQTIAEAKNPASQDIIRA
                     HLMIQDKYALPVACFVMALIALALGVSNRKDGYLASFAIGFIVVFVYYVLMYMSRAAA
                     TGGVLNPDISSWIPPAVLAAVGVVLLIWRARSTDRPLLIAWSVRRARPSDTPDETDSS
                     TSRSARWLFSAPRFGVPGTRLLDVYTAQQYLRIFALSIFGALGIFYISTFIDLADKLF
                     RGSATTTLLLRYFYYQTPQYVYYIIPIAGLLATLVTVGLMTKNSELVVMKACGMSLYR
                     VSAPLILLGVTASLLVFGVQEIVLARSNKEADRLNGIIRNWPQPTSSAVNRWIASESG
                     DIYHYDFFDGRT"
     gene            1755..>2135
                     /locus_tag="VH419_04890"
     CDS             1755..>2135
                     /locus_tag="VH419_04890"
                     /inference="COORDINATES: protein motif:HMM:NF015052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042986.1"
                     /translation="MTRVALLWHMHQPYYEDLVTREHFLPWVRLHALKDYYGMAALLR
                     EFPGVRVTFDLVPSLLVQLEAFAENRARDRYLELSLKPAEELSEPDVAFVLDNFFHAQ
                     RHRMIYVYPRYRELLDKRGRGLFAV"
BASE COUNT          424 a          653 c          691 g          367 t
ORIGIN      
        1 ggtgcggccg tcgaagaagt cgtagtgata gatgtcgccc gactcgctgg cgatccatcg
       61 gttgacggca ctcgacgtgg gctgcggcca atttcgaatg atgccgttca gtcgatccgc
      121 ttctttattg gaccgggcca gcacgatttc ctgcacgccg aacaccaaca ggctcgcggt
      181 tacacccagc aggatgagcg gggccgacac gcggtacaga ctcatgccgc aggccttcat
      241 cacgacgagc tcgctgttct tcgtcatgag tccgaccgtc acgagcgtgg ccagcaggcc
      301 cgcgatcgga atgatgtagt acacgtactg cggcgtctga tagtagaaat accgcagcaa
      361 cagcgtcgtc gtcgcggagc ctcggaacag tttgtcggcg agatcgatga acgtggagat
      421 gtagaagatt ccgagggccc cgaagattga aagcgcgaag atgcgaaggt actgctgtgc
      481 cgtataaacg tcgaggagtc tcgtgcccgg gacgccaaac cgcggcgcgc tgaagagcca
      541 cctggcggac cgggacgtcg aggagtcggt ctcatccggc gtgtcgcttg gacgcgcgcg
      601 acgtaccgac cacgcaatca gcagcggacg gtccgtcgac cgtgcgcgcc agatcagcag
      661 gaccacgccc acggcggcga ggacggcggg agggatccac gaactgatat cagggttgag
      721 gacgccgccc gtggccgccg cccgcgacat gtacatcagc acgtaataca cgaacacgac
      781 gatgaagccg atcgcaaagc tggcgagata accgtccttg cgattgctga cacccagggc
      841 gagcgcgatc agcgccatca cgaagcacgc gaccggcagc gcgtacttgt cctgaatcat
      901 caggtgcgca cggatgatgt cctggctggc cgggttcttg gcctcggcga tcgtctggcg
      961 cagttccgcg atcgtcatct cgggcgcgtt tcgcgacggg ggcttcggaa acacctcctc
     1021 cgcgtcgagc gtcacgctgg tgctttcgaa ctgcgcgccg tcgtacacgt cgggatccga
     1081 cgcgcgtgtg agatggtgcg ttcctttcag gagctgcagc tgaacgaact tctcgttgcg
     1141 atcgacgacg atgcggcctt cgcgcgcgaa atacaccatc gtctggccgg gcgtgtgcga
     1201 atcggcgaag aagacgtcct gccagagacc gccggcgagc acgttgcgga cgtacagcac
     1261 cgtattcgga aacctcgtga aaaagtcccg cggcttgacc tcgttctgga cctgcgattt
     1321 cacgacaccc agcgtgatct cgcggaagct ctgattggcg ttgggcagcg ccacgatggt
     1381 gacgtacgcc gtgccgatgc tcgccgccag cgcgatgatc ccgaccgggc ggagcagccg
     1441 atagatgctc acgccgcacg cctgcatcgc gacgaactcg cgatccgcgg agaggcgtcc
     1501 gaacgcaatc agcagcccga ggagtgtggc aatcgggatg ctgatgctga gcgcctgcgg
     1561 cagcagcgtg accagcacgc ggagcacgat cggcagctgc atgcccttgg cgaggaattg
     1621 atagccgacc tgaaggacgg gaggaatgat cagaagaaac gtcagcagcg cgagcgacag
     1681 caggagcggc agcacggcgt cgcgtacgac atatcgatcg aagatcgaga acactcgcta
     1741 ctagactaca acgtatgacc cgcgtcgccc tcctctggca catgcaccag ccgtactacg
     1801 aggacctcgt cacgcgcgag cacttcctcc cgtgggtgcg gttgcacgcg ctgaaggact
     1861 actacgggat ggcggcgctg ctgcgcgagt tcccgggcgt acgtgtgacg ttcgacctcg
     1921 tgccgtcgct gctcgtgcag ctggaggcat ttgcggaaaa tcgcgcgcgc gatcgctatc
     1981 tggagctgag cctcaagccg gcggaggagc tgagcgagcc ggacgtcgcg ttcgtcctcg
     2041 acaacttctt tcatgcgcag cgtcaccgga tgatttacgt ctatccgcgc tatcgcgaac
     2101 tgctcgacaa gcgcggacgc ggtctcttcg cggtc
//
LOCUS       DATBFZ010000165         8697 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_6291159, whole genome shotgun sequence.
ACCESSION   DATBFZ010000165 DATBFZ010000000
VERSION     DATBFZ010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 8697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8697
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6291159"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(557..2011)
                     /locus_tag="VH419_04895"
     CDS             complement(557..2011)
                     /locus_tag="VH419_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017935905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY7042987.1"
                     /translation="MSSTITTAVPARTYPSLRAAALPLLALCLAFFVEMVDNTLLTIA
                     LPTIGRDLGSGTTALQWITGAYSLTFGGLLLTAGSTADRLGRKRVLQAGLAAFGIISL
                     GVIFVTNAAGLIALRAALGLAAAAMAPVTMSLIFRLFDDEKLRMRAITVVMVVGLSGF
                     VLGPILGGTALAHVSWEWLLLANAPIALIAWIGVRLGVPADRPEDLTSDPLDLEGTLL
                     SIVGVGLGCYTLTSGVEHGWLSGITLVSAVGALAAAVAFVHHERAAVFPMLDLRLFSN
                     GPARGAAITQMGASITMAAVMFGLILHFQYAYGWSPMRAGLANLPLIITMIAATPLAE
                     GLVERFGHRVACLIGTAFLVASLLGMAWGVEHGYAAIAVSMVILTIGLRTIMTICAVA
                     LVEAMPGNRTSIGAALNDTAQEIGTSIGTAVVGTMIAALVTAHLPAGAWSSQLAQSLF
                     HGERLTFLLLAAIVGVVATYGSLSLTDSHSTDEH"
     gene            2112..2813
                     /locus_tag="VH419_04900"
     CDS             2112..2813
                     /locus_tag="VH419_04900"
                     /inference="COORDINATES: protein motif:HMM:NF014906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HEY7042988.1"
                     /translation="MTPRPDRTRSPRRRTSHSMESVLNETIALLDEAGEPALTIRALA
                     ARLGGGVASVYWYVSSREELLDRATDEVMGRVLADTAHLAAGKDPIANLRALSLALFD
                     EMLRRPWFGSYMLRNTGLLPNSMLMFDRLGQQLMRLDLTPRQRFHAVSSIISYVVGVA
                     TDMAQPPPKEFLDSGLEPAAFLKLYADRWRALDPEQYPFAHQIADEFERHDDAEVFRS
                     GLDLLLDGLRLQARS"
     gene            2922..3293
                     /locus_tag="VH419_04905"
     CDS             2922..3293
                     /locus_tag="VH419_04905"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY7042989.1"
                     /translation="MPTMGPTKTTDIDRVAASLLKLARLAKGLSQRALAATAGVPHST
                     VARIESGAMQPTLPLLYRLLAAADLEPRIRLEAYDDHDDVLDALARRAPERQAELEHT
                     RDATLAALEKTQPTKPRRRAG"
     gene            3283..3804
                     /locus_tag="VH419_04910"
     CDS             3283..3804
                     /locus_tag="VH419_04910"
                     /inference="COORDINATES: protein motif:HMM:NF039330.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6036 family nucleotidyltransferase"
                     /protein_id="HEY7042990.1"
                     /translation="MPAEPPDLDAPTILAILDRHHVDYVVVGGYAAQLHGSTRVTTDI
                     DLTPDRTPANLARLAAALRDLGGGIRVDDLDHGLSFDTSPEALAGTKTLNLRTAHGDI
                     DLTFEPDGTDGYRDLIRNADHHTIGTLHVYVAALADLIRSKTAAGRPKDLRALPELIQ
                     IQQAFDTNPTERR"
     gene            complement(3910..5304)
                     /locus_tag="VH419_04915"
     CDS             complement(3910..5304)
                     /locus_tag="VH419_04915"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY7042991.1"
                     /translation="MRVDDLRPLPIFDGLNDSQLAELVDAGAEVAIAPGVDLFREGDH
                     ADFWWVLLDGALDLVRHVGREDVVVARMDVPGRWAGGFRAWDEQGIYLATGRGAKPGR
                     VLRVPSQVLRERSDAWFPFAGHLIGGLYHTARSIESTARQRGALVTLGTLAAGLAHEI
                     NNPASAAARGVDAIEAASAALLSSLRQLADEEISPGQFAALDESRREIEPLSGDGDPL
                     ERADREQTLSAWLTRNGVEREWIIAGPLADAGVGISWCERVSTVLGPSALQSGLEWVA
                     STLSIAALLGEVKTSTQRVSQLVAAVRSYSQMDRASMQNVELTDGIESTLVMLAHKLR
                     DRGVEVVREYDERVPPIEAYAGELNQVWTNLIDNALDAMADGGTLRVSTSLEEGHVVV
                     EIGDTGTGIAPEVADRAFEAFYTTKDVGKGTGLGLDIARRIVVERHGGTITIDPRPGE
                     TVLRVRVPASATPA"
     gene            complement(5304..6962)
                     /locus_tag="VH419_04920"
     CDS             complement(5304..6962)
                     /locus_tag="VH419_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020578238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY7042992.1"
                     /translation="MAKPTILTVDDDPLVSAAIARDLSRKYGGDYRIVRATSGAEALS
                     VLAKLALRGDPVALIAADQRMPQMTGIEMLEQSRTHAPGAKLLLLTAYADTDVAIKAI
                     NDIGLDYYLLKPWDPPAERLYPVVDDLLGDWTQANPDHTADVRVVGHRWSDRSHELKL
                     FLARNHVPYRWYDIERDTEAARLIDLAAAAATDLPLVLVPDGDTLRSPSTLDLADALG
                     LRTRAEQPLYDVCIVGGGPAGLAAGVYAASEGLSTVIVEREAPGGQAGQSAAIENYLG
                     FPKGLSGSDLAQRALAQVSRFGAELVLARDVVGFEARGPVRAVLFDGGAEIEARAVIV
                     ATGVSYRRLDARGVDELTGRGVYYGANANEVNQCQGDDVYVVGAANSAGQAALNLSRF
                     AKRVVLVVRAATLLATMSHYLVERITAAANIEVRVHSEVVAARGDGHLEALTIVDRES
                     GVTDEVASSWLFVFIGATPRTDWLGPDVVRDDKGFVLTGQDLLVATQAPRWSLARPPF
                     TLETSVPGVFAAGDVRLDSMKRVASAVGEGAMSVYLVHRYLATI"
     gene            7290..7868
                     /locus_tag="VH419_04925"
     CDS             7290..7868
                     /locus_tag="VH419_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042993.1"
                     /translation="MYQLNNSIRPRYSRQLLTVAAGLGILAAAAGAGTSPSEAAVADY
                     TVWTSVPSACSPDEASTGRYDAHVSAFKHANGATGQLVTRCNVVNLPEFGLGDSQRLQ
                     AVYRDTDGVGTGQQVLVDLIQARPDGSTVILARLNSNSFSGSQASQTRSVGYTHNFDF
                     EHNAYYVEVRVTRSSTSTTPAASTVRLTSFAS"
     gene            8121..8693
                     /locus_tag="VH419_04930"
     CDS             8121..8693
                     /locus_tag="VH419_04930"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HEY7042994.1"
                     /translation="MDRVYRVGYFVGSLSSTSINRTLSKALIRLAPRDMEFTEIPIGN
                     LPLYSPDHDQNYPPEAVALKDAIASSDGVLFVTPEYNRSIPGALKNAIDWASRPWGQN
                     SFHHVPAAVCGASPGQIGTALAQQSLRGVLSFCNARQMTAPECYVTMRPGLITDDGEV
                     TDDSTTDFLTGFLQEFRIHIERVLTVIPRS"
BASE COUNT         1497 a         3049 c         2812 g         1339 t
ORIGIN      
        1 gtcggtgacg ccgtccgggt tggtgatctg gataacgtgc ccgctgcgga ggtccgcgac
       61 ccaggtgctt atcgcatcga ccgtgacatg ggtgtagacc accgagctac gtcggactcc
      121 gccgagctct gccgtccaga tctcgacaag ctcatcgcgc gagctgcaac attgcaacag
      181 gtggactccc atcgtgccga cagactgtgg gtggaaatag accgacgact gacacgactt
      241 gcgtgtgggt gccaaccgtc acgcccgcgg aaaccgtcct gctctctccc cgggtttctc
      301 actaccaaca acatccggtt tgggggccgc tcatcgacca gttgagcgtc gaatgagcca
      361 ccgcaatcgc cggcgtcggc gacagcggac cgttccggac ggcccgggaa aggagccgga
      421 cgatgagcca gctgccagtc cgggctctcg tcgacgaagg ctccggacgt cacttggcga
      481 aggtgtgtgg aggcgaagct gaccgggccg cgccctaccc aggagcgtcc ggtgcagctg
      541 gtccgggctg caggtgtcag tgctcgtcgg tggagtgcga gtcggtgagc gagagcgacc
      601 cgtaggtggc gaccacgccc acgatggcgg cgagaagcag gaaggtgagg cgctcgccgt
      661 ggaagagtga ctgtgcgagc tgggagctcc aggcgccggc cggaaggtgt gcggtcacga
      721 gtgccgcgat catggtgccg acgaccgcag tgccgatgct ggtgccgatt tcttgggcag
      781 tgtcgttgag ggcggcgccg atcgaggtgc gattgcctgg catggcctcg acgagtgcca
      841 cggcgcagat ggtcatgatg gtgcgcaggc cgatggtgag gatgaccatg gagaccgcga
      901 tcgcggcgta cccgtgctcg acgccccagg ccatgccgag gagtgatgcg acgaggaatg
      961 cggtgccgat caggcaggcg acgcggtgtc cgaagcgttc gaccagccct tccgccagtg
     1021 gtgtggcggc gatcatggtg atgatcagtg gcaggttggc cagtccggct cgcatcgggc
     1081 tccagccgta cgcgtactgg aagtgcagga tcagaccgaa catgacggcg gccatcgtga
     1141 tcgaagcacc catctgcgtg atggcagcgc cgcgcgccgg gccgttggag aacaggcgca
     1201 ggtcgagcat cgggaagacg gcggcacgct cgtgatggac gaaggcgacc gcagccgcca
     1261 gggcccccac cgcggacacc agggtgatgc cggacagcca tccgtgctcg acgccactgg
     1321 tgagcgtgta gcagccgaga cccacgccga cgatgctgag cagcgttccc tcgaggtcca
     1381 gtgggtcgct cgtcaggtct tctggccggt ctgcggggac gccgaggcgt acgccgatcc
     1441 aggcgatgag cgcgatcggt gcgttggcca ggagcagcca ctcccagctc acgtgggcca
     1501 gggcagtgcc gccgaggatc ggtccgagta cgaatccgga caggcctacg accatgacca
     1561 cggtgatggc gcgcatccgc agcttctcgt catcgaagag gcggaagatc agcgacatcg
     1621 tgaccggcgc catcgcggcg gccgcgaggc cgagcgcggc gcgcagggcg atgagtccgg
     1681 cggcgttcgt gacgaagatg acgcccaggc tgatgatgcc gaacgcagcc aggccggcct
     1741 gcagcacgcg cttgcggccg agtcggtcgg cggtcgagcc ggcggtgagc agcaggccgc
     1801 cgaaggtgag ggagtaagca cccgtgatcc actgcagggc cgtggtgcca ctcccgagat
     1861 cgcgaccgat ggtcgggagg gcgatggtca gcaaggtgtt gtcgaccatc tcgacgaaga
     1921 aggccagaca caaggcgagc aacggcagcg ccgccgcgcg gagagatgga taggtccgcg
     1981 cgggcactgc ggtggtgatt gtggagctca tggctcacct cctggtcatc gaaccttgtt
     2041 cgattatcga acgatgttcc atacgataga acatcgttcg ataggatgca aatcatgacg
     2101 ggaagccggc catgactcca cgacccgacc gcacgcgcag tccccgccgg cggacctcgc
     2161 actcgatgga atccgtgctg aacgagacga tcgcccttct cgacgaggcc ggcgagccgg
     2221 cgctgacgat ccgcgcgctc gcggcgcgcc tcggcggcgg cgtggccagc gtctactggt
     2281 acgtctccag cagggaggag ctgctcgacc gggcgaccga cgaggtgatg ggacgggtcc
     2341 ttgccgacac cgcccatctc gcggctggca aggatccgat cgccaatctg cgcgctctct
     2401 cactcgcctt gttcgacgag atgctgcggc gcccgtggtt cggctcctac atgctgcgca
     2461 acaccggcct cttgcccaac tcgatgctca tgttcgatcg gctgggtcag cagctcatgc
     2521 ggctcgacct cacgccccgg cagcgttttc acgcggtgtc atccatcatc agctacgtgg
     2581 tcggagttgc gaccgacatg gctcagccac caccgaagga gttcctcgac agcggcctgg
     2641 aaccggctgc gttcctcaag ctgtacgccg accgctggcg cgcgctggat cccgagcaat
     2701 accccttcgc ccaccagatc gccgatgagt tcgaacgtca cgacgacgcg gaggtcttcc
     2761 gttccgggct cgacctgctc ctggatggac tgcggttgca agctcgctcg tgagaggcgg
     2821 ggaacagggg cgccacaaat gggagcaccg ggagttggcg gccctggccc ggcacagccg
     2881 tttgaacgct gatcggctat cactggggta ggatggacga catgccaacc atggggccca
     2941 ccaagaccac agacatcgac cgggtggccg ccagcctgct caagctcgcc cgcctcgcca
     3001 aaggcctgtc ccagcgggcc ctggccgcca cggcgggtgt cccgcactcc accgtcgcgc
     3061 ggatcgagtc cggcgccatg cagcccaccc tccccctgtt gtaccggctc ctggcagcag
     3121 cggacctcga gccccgcatc cggctcgagg cctacgacga ccacgacgac gtcctcgacg
     3181 ccctcgcccg ccgtgccccc gaacggcagg ccgaactcga gcacacccgc gacgccaccc
     3241 tcgccgccct cgagaagacc cagccgacga aaccgaggcg tcgtgccggc tgaacctccc
     3301 gacctcgacg cccccaccat cctcgcgatc ctcgaccgcc accacgtcga ctacgtcgtg
     3361 gtcggcgggt atgccgcgca gctccacggc tcgacccgcg tcaccaccga catcgacctc
     3421 accccggacc gcacgcccgc caacctcgcc cgacttgctg cggccctgcg cgacctcggc
     3481 ggcgggatcc gcgtcgacga cctcgaccac ggcctctcgt tcgacacctc ccccgaagca
     3541 ctcgccggca cgaagacact caacctgcgc accgcgcacg gagacatcga cctcaccttc
     3601 gaacccgacg gcaccgatgg ctaccgcgac ctcatccgca acgccgacca ccacaccatc
     3661 ggcaccctcc acgtctacgt cgccgccctc gccgacctca tccgcagcaa gaccgccgcc
     3721 ggacgtccca aggacctgcg agcccttccc gaactcatcc agatccagca agcattcgac
     3781 accaacccaa ccgaacgccg atgattgcct gtgctctacg ccctgcgcgg ctgcgtcgca
     3841 accagcttgc atccggaaag gtggcaccag agcttccgga agttgctggc tctgctcgga
     3901 ctcaaccggc taggccggag tcgcgctggc gggtacacgg acccgtagca cggtctcacc
     3961 aggccgggga tcgatcgtga tcgtcccacc gtgccgttcg acgacgatcc gccgcgcgat
     4021 gtcgagcccg aggccagtgc ccttccccac gtctttggtg gtgtagaacg cctcgaacgc
     4081 ccggtccgcg acctctggcg cgatgccggt accggtatcg ccgatctcga ccaccacatg
     4141 gccttcctcg aggctggtcg agacgcggag cgttccgccg tccgccatcg cgtcgagggc
     4201 gttgtcgatc aggttcgtcc agacctggtt gagctctccg gcgtacgcct cgatcggcgg
     4261 gacacgttcg tcgtactcgc gcaccacctc gacgccgcga tcacgcagct tgtgcgcaag
     4321 catcaccaac gtgctctcga tgccatcggt gagctcgacg ttctgcatgg acgcccgatc
     4381 catctgcgag taggagcgga ccgctgccac gagctgcgac acccgctgcg tcgacgtctt
     4441 cacctcgccc agcaaagcgg cgatcgaaag cgtgctcgcg acccattcga ggccggactg
     4501 gagcgccgat ggaccgagga cggtgctcac ccgctcacac caggagattc ccacccctgc
     4561 gtcggctagg gggccggcga tgatccactc gcgttcgaca ccgttacggg tcaaccaggc
     4621 cgacaacgtc tgctcccgat cggcacgctc cagcgggtcg ccgtcgccgg acagcggttc
     4681 gatctcccgg cgcgactcat cgagagccgc gaactggccc ggcgagatct cctcgtccgc
     4741 gagttggcga agcgaggaca gcagcgcagc cgaggcggct tcgatggcgt cgacgcctct
     4801 cgcggccgcc gacgccgggt tgttgatctc gtgcgccagg cccgcggcga gggtgcccag
     4861 ggtcacgagc gcgccacgtt gtcgagcggt cgactcgatg gaccgtgcgg tgtggtagag
     4921 gccgccgatc aggtggccgg cgaaggggaa ccaggcatcc gaccgctcgc gcagcacctg
     4981 cgaggggacg cggagcaccc gccctggctt cgcacctctc ccggtggcga ggtagatccc
     5041 ctgctcgtcc caggcgcgga agccgcccgc ccaacggccg ggcacgtcca tcctcgcgac
     5101 cacgacgtcc tcacggccca cgtgtcggac gaggtcgagt gcgccgtcga ggagcaccca
     5161 ccagaagtcg gcgtggtcac cttctcggaa caggtcgacg cctggtgcga tggcgacctc
     5221 cgcgccggcg tcgacgagct cggcgagctg gctgtcgttc aggccgtcga agatgggcaa
     5281 cgggcggaga tcgtcgacac gcatcagatc gtcgccaggt agcggtggac gaggtagacc
     5341 gacatcgcgc cctcgccgac ggccgacgcc acccgcttca tcgagtcaag gcggacgtcg
     5401 cctgccgcga agacccccgg gacgctggtt tcgagggtga acggaggtcg cgccagcgac
     5461 caccggggcg cctgcgtcgc caccagtaga tcttgaccgg tgaggacgaa acccttgtcg
     5521 tcgcgcacga cgtccggacc cagccagtcg gtgcgagggg tcgcgccgat gaacacgaac
     5581 aaccagctcg aggcgacctc gtcggtgacg ccggattccc gatccacgat ggtgagcgcc
     5641 tcgagatgcc cgtcgccacg cgcggccacg acctcgctgt ggacgcgcac ctcgatgttc
     5701 gcggccgccg tgattcgttc gacgaggtag tgcgacatgg tggccagcaa cgtcgccgcc
     5761 cgtacgacga gcacgactcg cttggcgaag cgcgagaggt tgagcgccgc ctgtcccgcg
     5821 gagttcgcgg ctccgacgac gtacacgtcg tcaccctggc attggttgac ctcgttcgcg
     5881 ttcgcgccgt agtagacgcc acggccggtg agctcgtcga cgccacgtgc gtcgagccgt
     5941 cgatacgaga cgccggtggc gacgatgacc gcgcgcgcct cgatctcggc gccaccgtcg
     6001 aagagcaccg cgcgcaccgg tccgcgcgcc tcgaagccga cgacgtccct ggcgaggacg
     6061 agctcggccc cgaagcgcga gacctgtgcg agtgcacgtt gcgcgaggtc ggagccgctc
     6121 agccctttgg gaaagccgag gtagttctcg atcgccgcgc tctggccagc ctgtccgccg
     6181 ggtgcctcgc gctccacgat cacggtgctg agcccttcgg acgccgcgta gactcccgca
     6241 gccagtccgg ccggtccgcc accgacgatg cacacgtcgt acagcggttg ctccgcacgc
     6301 gtgcgcaggc cgagcgcatc ggcgaggtcg agcgtcgacg gtgaacgcaa cgtgtcgccg
     6361 tccgggacca gcaccaacgg aaggtcggtg gcagcggcgg cagccaggtc gatcagtcgc
     6421 gcggcctcgg tgtcgcgttc gatgtcgtac caccggtagg gcacgtggtt gcgcgccagg
     6481 aacagcttga gctcgtggct gcgatcggac caccggtgac cgacgacgcg cacgtcagcg
     6541 gtgtggtccg ggttggcctg ggtccagtcg ccgagcaggt cgtcgacgac cggatagagc
     6601 cgttcggccg gcggatccca cggcttgagg aggtagtagt cgagcccgat gtcgttgatc
     6661 gccttgatcg cgacgtcggt gtcggcatac gcagtgagga ggagcagctt ggcgcccggc
     6721 gcatgtgtac gggactgctc gagcatctcg atcccggtca tctgcggcat ccgctggtcg
     6781 gcagcgatca acgccaccgg atcgccgcga agggcgagct ttgccagtac ggacagcgcc
     6841 tctgcgcccg acgtcgcgcg gacgatgcga tagtcgccgc cgtatttgcg agacagatcc
     6901 cgggcgatgg ccgcggacac gagcggatcg tcgtctacgg tgaggatcgt cggcttcgcc
     6961 atgcggtcaa ccttcccccg atcaccgccg ttcggcgcgc gtttcgccag aatctgcctc
     7021 gcgggctcct tcaacgtcga cagccgtgcg tttgcttcga catctcttcg gaatgccagg
     7081 tgtccaggcg gatctgccca cgcagccaag cggcggctca cgtcgactgc atcggccggg
     7141 gtcgggcgac tgtttaccgt cgcgataccg gatgaacacc atctcgattc ccatccggag
     7201 gagaactgag ctgcgccacc gaagccgtgc atcaccccgg ttgattggcg ctgacaattt
     7261 cccaccggat ccttcgaaag gggatcgaaa tgtaccagct caacaactcc attcgccctc
     7321 ggtactcccg tcagctgctc acagtggcgg ccgggctcgg gatcttggcc gccgccgctg
     7381 gcgccgggac atctccgtcc gaggccgcgg tggccgacta caccgtgtgg acgagcgtcc
     7441 cctcggcctg ctcgcccgac gaagcgagca ctgggaggta cgacgcccac gtctccgcgt
     7501 tcaagcacgc caacggcgcc accgggcagc tcgtgacccg atgcaacgtt gtcaacctgc
     7561 ccgaattcgg cctcggcgac agccaacggc tgcaagccgt ctaccgcgac accgatggcg
     7621 tcggaaccgg gcagcaagtc ctcgtcgacc tcatccaggc ccgcccggat ggttcgacgg
     7681 tcatcctcgc ccggctcaac agcaactcct tctccggaag ccaggccagt cagacccgct
     7741 ccgtcggcta cacgcacaac ttcgacttcg agcacaacgc ctactacgtc gaggtccgcg
     7801 tgacccgcag ctccacgtca acaacgccag cagcgagcac tgtccgactc acctccttcg
     7861 ccagctgagc cccacccgag cctctctccg caaccacccg gatagctccc acctcactcg
     7921 atctactcgg accgggtcgg tcgcggggag aggctccatc gtccatgccc ccgaggaata
     7981 tgtcatgacc acgcccgcct tcccgaccag gagggcaccg cgcccgccgc ttgtccctcc
     8041 gacgtcaacc cgccagtctg gcagcgtggg ctacgccctc gggcgccgcg cccgaggtcc
     8101 ggactcggaa ggagacagcc atggaccgcg tctatcgggt tggctacttc gtcggcagtc
     8161 tctcgtcgac ctcgatcaac cggacactca gcaaggcgtt gatcaggctc gcaccgcgag
     8221 acatggagtt caccgagatc ccaatcggca acctccctct ctacagtccg gaccacgacc
     8281 agaactaccc gcccgaagcg gtcgcgctca aggacgccat cgcgtcgtca gacggggtgc
     8341 tgttcgtgac accggagtac aaccggtcga tacccggggc actcaagaac gccatcgact
     8401 gggcatcgcg accctggggc cagaattcgt tccaccacgt gccagccgcg gtctgcggcg
     8461 cctcgcccgg acagatcggt accgcgctcg cgcagcagag tctgcgtggc gtgctcagtt
     8521 tttgcaacgc ccgccagatg accgcacccg agtgctacgt caccatgcga cccggcctga
     8581 tcaccgacga cggcgaggtc accgatgact ccacaaccga ctttctgacc ggcttcctcc
     8641 aggagttccg gatccacatc gaacgggtcc tgaccgtgat tccgcgctcg tgaagga
//
LOCUS       DATBFZ010000166         5553 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_1420701, whole genome shotgun sequence.
ACCESSION   DATBFZ010000166 DATBFZ010000000
VERSION     DATBFZ010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 5553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5553
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1420701"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..627)
                     /locus_tag="VH419_04935"
     CDS             complement(<1..627)
                     /locus_tag="VH419_04935"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEY7042995.1"
                     /translation="MTALVYTLGVVLFLLGVLGSIALHEVGHMVPAKKFGVKVTQYFV
                     GFGKTVWSTKRGETEYGIKLVPMGGFIRMVGMLPPAKRDADGRIKGFQGGFFGKLIAD
                     ARALEYENVTATEQDRLFYRKAWWKKVIVMSGGPMMNVLLAVLMLGTVFIGFGVNEPT
                     LVVDKVSDCVIPANQPDRPCRVSGPDADPAAPAREAGFRSGDEILAVNG"
     gene            complement(624..1937)
                     /gene="dxr"
                     /locus_tag="VH419_04940"
     CDS             complement(624..1937)
                     /gene="dxr"
                     /locus_tag="VH419_04940"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009074592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEY7042996.1"
                     /translation="MSPGTAPPVESSTRRDVVLLGSTGSIGTQTLDIVRANPDRFRVV
                     ALAAGGSNPGLLAAQAIEFGVPVVAVSRSTAIEDLQLALYAEAQRRGYHHGDFSLPRI
                     VGGPDAAEQCAALPCEIVLNAIDGAAGLKATIVALRSGATLALANKESLIIGGELVTS
                     LAKPGQIVPVDSEHSALAQCLRGGRADEVRRLVITASGGPFRGRTRDELADVSAADAL
                     AHPTWDMGRLVTTNSSTLVNKGLEVIEAHLLFAIDFDRIEVVVHPQSIIHSMVEFHDG
                     STLAQASPPDMRLPIALALGWPDRVADVAAAVDWSQSQRWEFEPVDHEAFPGLRLAIE
                     AGRRGGLGPAIFNAANEVCVTAFLEDRLPYLGIVDTLAAVLAADDVPSYEGRLGAETG
                     DGAVEESLDAVLAADRWARDRAADLIGSAKLAATTLPLPGGDSRP"
     gene            2064..2738
                     /locus_tag="VH419_04945"
     CDS             2064..2738
                     /locus_tag="VH419_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7042997.1"
                     /translation="MLNSIRRHLTTILVAMVTAIVTAGAPALAQGVQNVVVADNSDKV
                     DGKHAVGAGASVAQRAGKLVATNQNGRLPDSIIAKAPDANLVDGIDSANLLPGGTLPH
                     GVTIRGHYANMGTTDGANLQSAAEGISFGYRLASAPTPVVIHAGEPQTADCPGTAANP
                     DAAPGFLCVYENDAQNKRDASYPHVAVGFAGGSTTPFGAYIYTQSNTAAPAFFWSRGS
                     WAVTAP"
     gene            complement(2841..4283)
                     /locus_tag="VH419_04950"
     CDS             complement(2841..4283)
                     /locus_tag="VH419_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020662708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY7042998.1"
                     /translation="MTRAFPMWIGGKQVDGPESLEITSPYDARPVGTTSWASPEQVDD
                     AIAAADGVKDEAAGLPIHVRADALSHVSGQIAVRADEIAELITGENGKPMVWSRAEVG
                     RAVSVFRIAAEEARRWSGEAIRLDTDKAAVGRLAYVRRVPRGPVLAISPFNFPLNLVA
                     HKVAPAIAVGAPLVLKPAPKTPLSALLLAEIIAETDLPAGMVNVVNVQNDQAGDLVAD
                     ERLPVVSFTGSGPVGWSIQKAVPHKQVTLELGGNAAAVVCSDWTSDSDLDWAATRIAT
                     FSNYQAGQSCIGVQRVYVAGPAYDDFKQRLVSKVEDLKTGDPYDEATQVGPVINAQAA
                     DRIMAWVQEAVDQGASMLTGGGRDGSTVEPIVLEGVAADAKVSCDEVFGPVVLVERVA
                     DDAAAFAKVNDSAYGLQAGVFTHDLQKAFHAHRELEVGGVIVGDVPSYRADQMPYGGA
                     KDSGVGREGIRYAMQDYTYERVMVLTGLDL"
     gene            4437..>5553
                     /locus_tag="VH419_04955"
     CDS             4437..>5553
                     /locus_tag="VH419_04955"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008357666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HEY7042999.1"
                     /translation="MSDVPTGGPELPQERRVVTEIPGPRSRELHARKEAAVSGGIGVG
                     LPVYVTAAGGGVVVDADGNSLIDMASGIAVTGVGNAAPAVVERVSAQVEQFTHTCFMV
                     VPYAGYVEVCEQLNELTPGDHAKKSVLLNSGAEAVENAVKIARTHTGRNAVVVFDHAY
                     HGRTNLTMAMTAKNVPYKDGFGPFAGEVYRAPMSYPFREETSISGEAAADRALTIVEK
                     QVGANNVACVVVEPILGEGGFVVPAPGFLPRLAEWCRDNDVVFVADEIQTGFCRTGDW
                     FACEHEGVVPDLVTTAKGIAGGLPLAAVTGRAEIMDAVKPGGLGGTYGGNPVACASAL
                     GAIATMREQDLAARARHIGELMLGRLNEFAERYPVIGD"
BASE COUNT          875 a         1943 c         1891 g          844 t
ORIGIN      
        1 gccgttgacc gcgaggatct cgtcgccgga ccggaagcct gcttcgcgcg cgggggcggc
       61 cggatcggcg tctggaccgc tgacgcgaca tgggcggtcc ggctggttgg cggggatgac
      121 gcagtcggac accttgtcga cgacgagcgt gggttcgttc acgccgaagc cgatgaacac
      181 cgtaccgagc atgagcaccg ccagcaggac gttcatcatc gggccgccgg acatgacgat
      241 gaccttcttc caccacgctt tgcgatagaa gagccgatcc tgttcggtcg cggtgacgtt
      301 ctcgtactcg agcgcacggg cgtcggcgat cagcttgccg aagaaaccgc cttgaaagcc
      361 cttgatccga ccgtcggcgt cccgcttggc cggtgggagc atgccgacca tgcggatgaa
      421 cccgcccatc gggacgagct tgatgccgta ctccgtctcg ccacgcttgg tggaccagac
      481 ggtcttgccg aagccgacga agtactgcgt caccttcacg ccgaacttct tcgccggcac
      541 catgtggccg acctcgtgca gcgcgatcga gccgagaacg ccgagcagga agagcacgac
      601 acccaaggtg tagaccagtg cggtcatggt cggctgtctc ctcctggcag cggcaacgtc
      661 gtggcggcga gcttggcgct gccgatgagg tcggccgcgc ggtcacgggc ccagcggtca
      721 gccgccagga ctgcgtcgag gctctcctcg accgcgccgt cgcccgtctc ggccccgagt
      781 ctgccctcgt acgagggtac gtcgtcagcg gccaggacgg cagccaaagt gtcgacgatg
      841 cccaggtacg ggagcctgtc ctcgaggaac gctgtgacgc agacctcatt cgcggcattg
      901 aagatcgccg ggccgaggcc accacgtctt cctgcctcga tcgcgagccg cagaccagga
      961 aaggcctcgt ggtcgaccgg ctcgaactcc catcgctgcg actgtgacca gtcgaccgca
     1021 gcggccacgt cggccacccg gtcgggccag cccaacgcca gtgcgatagg cagccgcatg
     1081 tcgggcgggg acgcttgcgc gagcgtggag ccgtcgtgga actccaccat cgagtggatg
     1141 atcgactgag ggtggacgac gacctcgatc cggtcgaagt cgatggcgaa cagcaggtgc
     1201 gcctcgatca cctcgaggcc cttgttgacc aacgtcgaag agttcgtcgt caccagccga
     1261 cccatgtccc aggtcggatg cgccagcgcg tccgccgccg acacatcggc gagctcgtcg
     1321 cgcgtccttc ctcggaaggg tccgccgctt gcggtgatga cgagccttcg gacctcgtcc
     1381 gcccggccac ctcgaaggca ctgggccagt gccgagtgct cggagtccac cggcacgatc
     1441 tgaccaggct tcgccaacga ggtgacgagc tcaccaccga tgatgagcga ctccttgttg
     1501 gcgagcgcca gcgtcgcgcc ggaccggagg gcgacgatgg tggctttgag cccagccgcg
     1561 ccgtcgatcg cgttgagcac gatctcgcac ggcagcgccg cgcactgctc ggcggcgtcc
     1621 ggaccgccga cgatgcgcgg caggctgaag tcaccgtggt ggtatccgcg ccgctgcgcc
     1681 tcggcgtaca acgcgagctg gaggtcctcg atggcggtgc ttcgcgagac cgcaacgacc
     1741 ggtacgccga actcgatggc ctgcgcggcc agcaggccgg gattgctacc gccggcggcc
     1801 agcgcgacca cacggaaccg gtccgggttc gcccgcacga tgtcgagcgt ctgggtcccg
     1861 atcgagcccg tcgaccccag cagcacgacg tctcgacgag tgctcgactc gacggggggg
     1921 gcagtccccg ggctcatgcg aagagtgttt cacgccagga ccgtgacgct cgcaccgtca
     1981 cagcgtgccg ttcccgaggc gcgtgtatct gccggatccg tcgcggcctc ctccgcacga
     2041 acccgaccag ccatggagga gccgtgctca acagcatccg cagacacctg acgacgatcc
     2101 tggtggccat ggtcaccgcg atcgtgaccg ccggcgctcc cgccctggcg caaggcgtgc
     2161 agaatgtcgt ggtcgccgac aactccgaca aggtcgacgg caaacatgcg gtcggcgcgg
     2221 gagcaagcgt cgcccagcgg gcgggcaagc tcgtggccac gaaccagaac ggcagactcc
     2281 ccgactccat catcgccaag gcacccgacg ccaacctggt cgacggcatc gactccgcca
     2341 acctgctccc cggcggcacc ctccctcatg gcgtcacgat ccgcggccac tacgcgaaca
     2401 tggggaccac cgacggcgcg aacctgcaat cggccgccga gggcatctcc ttcggatatc
     2461 gactcgcgtc cgcgccgact ccggtggtca tccacgccgg tgaaccacag accgcggact
     2521 gcccaggcac ggcggccaac ccggacgcgg cacccggctt cctgtgcgtg tacgagaacg
     2581 acgcccagaa caagcgcgat gcgagctacc cgcacgtcgc cgtcgggttc gccggtgggt
     2641 cgacgacgcc cttcggcgcc tacatctaca cccagtccaa cacggcagcg ccagccttct
     2701 tctggtcacg cggaagctgg gccgtcaccg cgccctgagt gtggcggtct cgtccgagcg
     2761 gatgctctcg accgcacaca acgcgtcgac cgaccaatcc tggacgtgat cggccgtccg
     2821 acaggtgcgg cgagtcgacc tcagagatcg aggccggtga gcaccatcac gcgttcgtag
     2881 gtgtagtcct gcatggcgta ccggatgccc tcgcggccga cgccggagtc tttggcgccg
     2941 ccgtacggca tctggtcggc ccggtagctc gggacgtcgc cgacgatcac gccgccgacc
     3001 tcgagctcgc ggtgggcgtg gaacgccttc tgcaggtcgt gggtgaagac gccggcctgc
     3061 aacccgtacg ccgagtcgtt caccttcgcg aaggcggcgg cgtcgtcggc gacccgctcg
     3121 acaaggacca ctgggccgaa gacctcgtcg cacgacacct tcgcgtccgc cgcgacgccc
     3181 tcgagcacga tcggttcgac cgtggagccg tcccgtcccc cgccggtcag catgctggcc
     3241 ccctggtcga ccgcctcctg cacccacgcc atgatgcggt cggcggcctg ggcgttgatc
     3301 accgggccga cctgggtcgc ctcgtcgtac gggtctccgg tcttgaggtc ctcgaccttc
     3361 gagacgagcc gctgcttgaa gtcgtcgtac gccggaccgg cgacatacac gcgttgcacg
     3421 ccgatgcagg actggcctgc ctggtagttg gagaaggtcg cgatccgggt ggcggcccag
     3481 tcgagatcgc tgtcggacgt ccagtccgag cacacgacgg ctgcggcgtt gccgccgagc
     3541 tcgagcgtca cctgcttgtg cggaaccgcc ttctggatcg accagccgac ggggccggac
     3601 ccggtgaacg agacgaccgg cagccgctcg tcggcgacga ggtcgcccgc ctggtcgttc
     3661 tggacgttga cgacgttcac catcccggcc ggcaggtcgg tctcggcgat gatctcggca
     3721 agcaggaggg ccgacagcgg cgtctttggt gccggtttga ggaccagtgg tgcgccgacg
     3781 gcgatggccg gggcaacctt gtgggccacc aggttgagcg ggaagttgaa cggcgagatc
     3841 gcgagtaccg gtcccctcgg tacccgacgc acgtacgcga gccgcccgac tgccgccttg
     3901 tcggtgtcga gccggatggc ctcacccgac cagcgtctcg cctcctcggc ggcgatccgg
     3961 aagaccgaga cggcgcgtcc gacctcggca cgcgaccaca ccatcggctt gccgttctcc
     4021 cccgtgatca gctccgcgat ctcgtccgcc cggacggcga tctggccgct gacgtgcgac
     4081 agcgcgtcgg cgcggacgtg gatggggagt ccggcggcct cgtccttgac gccgtccgct
     4141 gctgcgatcg cgtcgtcgac ctgctcgggg ctggcccacg acgtcgtccc caccggacgt
     4201 gcgtcgtaag ggctggtgat ctccagggac tcggggccgt cgacctgctt gccaccgatc
     4261 cacatcggga atgctcgcgt catacctctg acgctagtgc cctgtgcaag gccctgccgc
     4321 cgcagtgccc gccccatgct gcctgcccgc ccttgccagc ccgtccctgt cagcccgtcg
     4381 cgccggccgt tctgccgcgc ggttccagaa ggcgccggac ggcgtacggt agcgccatga
     4441 gcgatgtccc gacgggagga ccggagctgc cgcaggagcg ccgcgtcgtc accgagatcc
     4501 ctggtccgcg gtcacgagag ctgcacgccc ggaaggaggc tgccgtctcc ggcggcatcg
     4561 gggtcggcct acctgtgtac gtcaccgccg ccggcggtgg tgtggtcgtc gacgccgacg
     4621 gcaactcgtt gatcgacatg gcgtccggga tcgcggtgac cggcgtgggc aacgcggcgc
     4681 cggcagtcgt cgagcgggtc tcggcacagg tcgagcagtt cacgcacacc tgcttcatgg
     4741 tcgtgccgta cgccgggtac gtcgaagtgt gcgagcagct gaacgagctg acgccgggcg
     4801 accacgcgaa gaagtccgtg ctgctcaaca gcggtgccga ggcggtggag aacgccgtca
     4861 agatcgcccg aacgcacacc ggccggaacg ctgtcgtcgt gttcgaccac gcctaccacg
     4921 gacgcacgaa cctgacgatg gcgatgacgg cgaagaacgt gccgtacaag gacggcttcg
     4981 ggccgttcgc cggggaggtc taccgcgcgc cgatgtcgta cccgttccgc gaggagacgt
     5041 cgatctcggg tgaggcggcc gcggatcgcg ccctgacgat cgtcgagaag caggtcgggg
     5101 cgaacaacgt ggcgtgcgtg gtggtcgagc cgatcctcgg cgaaggcggc ttcgtcgtac
     5161 cggcaccggg gttcttgccg aggctcgccg agtggtgccg cgacaacgac gtcgtcttcg
     5221 tcgccgacga gatccagacc ggcttctgcc gcaccggtga ctggttcgcg tgcgagcacg
     5281 aaggcgtcgt acccgacctc gtcacgaccg cgaagggcat cgccggcggt ctgccgctgg
     5341 cggcggtgac cggacgcgcg gagatcatgg acgccgtcaa gcccggcgga ctcggcggga
     5401 cgtacggcgg caacccggtg gcctgcgcct cggccctggg cgcgatcgcc accatgcgcg
     5461 agcaggacct ggctgcccgg gcccgccaca tcggggagct gatgctgggg cggctgaacg
     5521 agttcgctga gcgctacccc gtgatcggtg acg
//
LOCUS       DATBFZ010000167         1668 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_1098798, whole genome shotgun sequence.
ACCESSION   DATBFZ010000167 DATBFZ010000000
VERSION     DATBFZ010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1668
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1098798"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..156)
                     /locus_tag="VH419_04960"
     CDS             complement(<1..156)
                     /locus_tag="VH419_04960"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY7043000.1"
                     /translation="MSAQGQLDGQTVVVIGGSSGIGRATARLARTEGADVVITGRDHG
                     RLEEAAAD"
     gene            complement(153..449)
                     /locus_tag="VH419_04965"
     CDS             complement(153..449)
                     /locus_tag="VH419_04965"
                     /inference="COORDINATES: protein motif:HMM:NF014229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="HEY7043001.1"
                     /translation="MLTACRHVDLEHTVQPSSWGCEECLRMGSSWVHLRLCMTCGHVG
                     CCDQSPNRHATAHWHEYPDHPVLRSYEPGEDWWWCYADQVAFEIDGAPPGPSHP"
     gene            complement(464..757)
                     /locus_tag="VH419_04970"
     CDS             complement(464..757)
                     /locus_tag="VH419_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043002.1"
                     /translation="MSFGSELNTILVATDGYAVPHAPSERDRVLLDEAAAQAAERGVN
                     ATSALRLGSTVASALLGSVSLGVLHKATRPVLIVRGAHSTHSSTTPPRAATSA"
     gene            complement(741..965)
                     /locus_tag="VH419_04975"
     CDS             complement(741..965)
                     /locus_tag="VH419_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043003.1"
                     /translation="MSTGNGRDVDVATLADLLRETSEHHGAFEAVTPKHDWWDWYAPY
                     LQARLDGKTSDQATQAADQYMKDVRHVVRQ"
     gene            complement(962..1444)
                     /locus_tag="VH419_04980"
     CDS             complement(962..1444)
                     /locus_tag="VH419_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012918821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY7043004.1"
                     /translation="MSSTETGSGTSAPTADPKAGEWRLEVIGLPVADVDRAKDFYVNT
                     LGWRLDADFNVNENFWVVQVTPPGSLCSIHFGKGITTAAPGSIDHMYLVVTDLEAARA
                     ELVDRGVEVSEIFHLLPGEDPKPGVDPAHGTYQSYATFADPDGNLWSLQEITTRLPGR
                     "
BASE COUNT          243 a          548 c          591 g          286 t
ORIGIN      
        1 gtcggccgcc gcctcctcca ggcgcccgtg atcgcgaccg gtgatgacga cgtcggcgcc
       61 ttcggtccga gccagccggg cggttgcgcg cccgatgccg gagctgccgc cgatcacgac
      121 gacggtctgc ccgtcgagct gtccctgggc actcatgggt gagagggccc gggcggagcg
      181 ccgtcgatct cgaacgccac ctggtcggcg tagcaccacc accagtcctc gccgggctcg
      241 tacgacctga ggacggggtg gtcggggtac tcgtgccagt gcgcggtggc gtgccggttg
      301 ggcgactgat cacagcaccc gacgtgtccg cacgtcatgc agagccggag gtggacccag
      361 ctgctcccca tgcggagaca ctcctcgcac ccccagctcg agggctgaac cgtgtgctcg
      421 agatccacgt gtcggcatgc cgtgagcatc ggtgatcgac cattcaggca gacgtcgcgg
      481 cacgtggtgg tgtggtcgac gagtgcgtcg agtgggcgcc ccggacgatg agcaccgggc
      541 gcgtcgcctt gtgcaggacg ccgagcgaca cgctgccgag cagcgcgctg gcgaccgtgg
      601 agccgagtcg gagtgcgctc gtcgcgttga cgccacgttc cgccgcctgc gcggcggcct
      661 cgtcgagcag gactcggtcg cgctcgctcg gtgcgtgcgg taccgcgtag ccgtcggtcg
      721 cgacgagaat cgtgttgagc tcactgccga acgacatgac gcacgtcttt catgtactgg
      781 tcggcggcct gcgtggcctg gtcggacgtc ttcccgtcga ggcgagcctg cagatacggc
      841 gcgtaccagt cccaccagtc gtgcttgggt gtgaccgctt cgaacgcacc atggtgctcg
      901 gacgtctcgc ggaggagatc ggccagggtc gccacgtcga cgtcgcggcc gttgccggtg
      961 ctcatcgccc cggcaaccgt gtcgtgatct cctgcagcga ccagaggttg ccgtcaggat
     1021 cagcgaaggt tgcatacgac tggtaggtgc cgtgcgccgg gtccacaccg ggtttcgggt
     1081 cctcgccagg cagaaggtgg aagatctcgc tcacctcgac accccggtcg accaactccg
     1141 cgcgggcggc ctcgaggtcg gtcaccacca ggtacatgtg gtcgatcgag ccgggcgctg
     1201 cggtcgtgat gcctttgccg aagtgaatgg agcacagcga gcccggcggt gtcacctgga
     1261 ccacccagaa gttctcgttg acgttgaagt cggcgtcgag gcgccacccc aacgtattga
     1321 cgtagaagtc cttggcccgg tcgacgtcag cgaccggcag cccgatcacc tcgagccgcc
     1381 attctccggc tttcgggtcg gcggtcggcg ccgacgtgcc gctgccggtc tcggtgctgc
     1441 tcatcgttcc ctcctcgggt tcgtccgaga cgaggctcgc acgactgagt ggtcgtcgcg
     1501 cccgtgagga tccctggtcg gcacgcgacc cgtggcttcc acgggttcgg ttggttctcg
     1561 cagtggggga tcctgacgcg agtcggacac gatcggagaa ggacatgacg gagtcggagc
     1621 accaggacta cgacgtcatc gtgttggggg caggtgcacc gggggagc
//
LOCUS       DATBFZ010000168         3964 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_1423790, whole genome shotgun sequence.
ACCESSION   DATBFZ010000168 DATBFZ010000000
VERSION     DATBFZ010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 3964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3964
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1423790"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(157..471)
                     /locus_tag="VH419_04985"
     CDS             complement(157..471)
                     /locus_tag="VH419_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012920537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEY7043005.1"
                     /translation="MRDIHIAQLDKARPVVVLTREPVRAAMRHVMVAPITTTVKGLST
                     EVPVGAANGVDQPGVASCDNIITIDKAALGHHVGFLFERQEHELTAAIVSAFALRTDE
                     LT"
     gene            complement(464..688)
                     /locus_tag="VH419_04990"
     CDS             complement(464..688)
                     /locus_tag="VH419_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020387341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HEY7043006.1"
                     /translation="MTTQITVRLPDELVAFVDRLVSEGAASRAAVVTRALNLYQQQVR
                     GEQDARILEKTGDYDDFDELVVHASIEPDA"
     gene            complement(738..1250)
                     /locus_tag="VH419_04995"
     CDS             complement(738..1250)
                     /locus_tag="VH419_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007077938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEY7043007.1"
                     /translation="MTETSTSADFEDLLAANRRFAESFALTGFDGVAHAGVAMVTCMD
                     SRIDPLGMIGLTAGDAKILRNPGGRVDDGVLVALVVGVNLLGVDRVLVVQHTRCAMAS
                     ATEPEMRDRLAQASGHDASWMSFGVMTDQRATLRDDVAKVRAHPLIDDHVLVGGFMYD
                     VDTGLLQPVD"
     gene            1433..2677
                     /locus_tag="VH419_05000"
     CDS             1433..2677
                     /locus_tag="VH419_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017932816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEY7043008.1"
                     /translation="MRSIDLSGLDKVPDSLQFPLQRAGVDPVAQLAAARESGPIYLLA
                     SVFGLHIWMVTGAEQNRVVLADSERFSTDIRRYVGKQDATGAQAIGGLGFTDPPDHTR
                     LRRLLTPEFTRRRLSRLQPRIEAVVSERLAALAARRADGDPVVDLVAEFAFPIPFVVI
                     CELLGIAPEDRDRFRELGHARFDVRQGGLGVFGAMSESRDFLIETAQQQRRHPRDGLI
                     GALIAEHGDAITDDELGGLADGVFTGGYETSASMIALGTLTLLQSPDAAAWLRAHDAA
                     AAPVVDELLRYLSVVQIGFPRFARQDMQLLGANVRAGDVVICSLSGANRDEAWAGAHA
                     DAFDPHRFDGARPMTSHLAFGYGMHRCVGAELGRMELLTAIPALLRRFPKMSLAVGPS
                     KLRFRDLSIVYGIDELPVRLGD"
     gene            complement(3208..>3964)
                     /locus_tag="VH419_05005"
     CDS             complement(3208..>3964)
                     /locus_tag="VH419_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020392473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY7043009.1"
                     /translation="RNLIHIKRMPESLMDVTIQPVMFVLLFAYVFGGAISVPGGGSYN
                     AFLLPGIMVQTIVFSSFVVAVGLTADLDKGIIDRMRSLPIARSSVLIGRSISSLIHSS
                     IGIVVMALTGLLIGWRIDNGVWNAVLAFALLLLWGFSMIWLGIWTGSTLRSVEAVQGF
                     MFTLMFPLTFVANTFVPTEGMPNWLQVIAEWNPVSALTQACRVLWGNGPEAPADAALP
                     LRYPEIATVCWVVLITAAFAPLAVRAFRRRARD"
BASE COUNT          668 a         1305 c         1353 g          638 t
ORIGIN      
        1 ggtcctgacc aggtacgaga ccaagcgctg agcgccgggc tctacttcaa cgcccgttcg
       61 tcaagcgccg ataacatatc ttatgtcaac tcgcatcacc atccgggggt gcgacccagg
      121 gctgcgaccg atcccccacc tctttcggcg aggcggtcac gtgagttcgt cggtgcgcag
      181 cgcgaaggcg ctcacgatcg ctgcggtgag ttcgtgctct tggcgctcga acaggaagcc
      241 gacgtggtga ccgagcgcag ccttgtcgat cgtgatgatg ttgtcgcagc tggcgacacc
      301 gggctgatcg accccgttgg ccgccccgac cggcacttcg gtgctgaggc ccttcacggt
      361 cgtcgttatc ggcgccacca tcacgtgacg catcgcggcc cgcaccggct cgcgtgtcag
      421 cacgacgacc ggacgcgcct tgtcgagctg cgcgatgtgg atgtcacgca tcaggctcga
      481 tggacgcgtg cacgacgagc tcgtcgaagt cgtcgtagtc cccggtcttc tccaagatcc
      541 gggcgtcctg ctcgcctcgc acctgctgct ggtagaggtt cagcgcccgg gtgacgactg
      601 cggcgcgaga cgcggcaccc tcgctcacca gccggtcgac gaaggcgaca agctcgtccg
      661 gaagccgcac ggtgatctgc gtcgtcatac cgaaatggtt ccagattggg atccacctgg
      721 gtcgagtgga agcgacatca gtcgacgggc tgcagcaggc cggtgtcgac gtcgtacatg
      781 aagccgccga ccaggacgtg gtcgtcgatc agcggatggg cgcgcacctt ggcgacgtcg
      841 tcgcgcagcg tggcccgttg atcggtcatg accccgaacg acatccagga ggcgtcgtgc
      901 ccgctggcct gggccagtcg gtcgcgcatc tcgggctcgg tcgcactcgc catggcgcac
      961 cgcgtgtgct ggacgacgag gacgcggtcg acaccgagca gattcacacc gacgacgagg
     1021 gcgacgagaa ccccgtcgtc gacacgaccg ccggggttgc gcaggatctt ggcgtcaccg
     1081 gccgtgaggc cgatcatgcc cagtgggtcg atccgcgagt ccatgcaggt gaccatcgcg
     1141 acgccggcat gagccacgcc gtcgaagccc gtcagggcga acgactccgc gaaccgccgg
     1201 ttcgctgcca gcaggtcctc gaagtctgcc gacgtactgg tctcggtcac gggcgtcacg
     1261 ctacaggcca ggggtacatc cggggtgtca ccgaggctgc cggcatggcc gatggagcat
     1321 gcttgggctt gagatccgcg agccatggtc acggcattga agaacggggt tgtgggtgtt
     1381 gggactgtcc acggttgtcc agcgaaccgg gagtctgctg tcacgacgtg cgatgaggtc
     1441 gatcgacctg tcggggttgg acaaggtgcc ggactcgctc cagttcccgc tgcagcgggc
     1501 gggggtcgat ccggtggccc aactcgccgc ggcccgggag tcgggaccga tctacctgct
     1561 cgcctccgtc ttcggactcc acatctggat ggtcaccggc gcggagcaga accgcgtcgt
     1621 cctggccgac tccgaacggt tcagcactga catccggcgc tacgtcggca agcaggacgc
     1681 caccggtgcc caggccatcg gcggcctcgg cttcaccgac ccgcctgacc acacgaggct
     1741 gcgtcggctg ctgacgccgg agttcacccg gcgccggctc agccgcctcc agccacgtat
     1801 cgaggctgtc gtgtccgagc ggctcgccgc gttggcggcc cggcgtgcgg acggcgaccc
     1861 ggttgtggac ctggtggccg agttcgcgtt cccgatcccg ttcgtcgtca tctgcgagtt
     1921 gctcggtatc gcaccggaag accgtgaccg attccgtgag ctggggcacg cgcggttcga
     1981 cgtacgacag ggcgggctgg gtgtgttcgg tgccatgtcc gagtcgcggg acttcctgat
     2041 cgagaccgcg caacagcagc gccggcatcc tcgagacggc ctcatcgggg cgctgatcgc
     2101 cgagcacggt gacgcgatca ccgacgacga gctgggcggg cttgccgacg gcgtgttcac
     2161 cggtggctat gagacgtcgg ccagcatgat cgcgctcggt acgttgacgc tcctccagtc
     2221 acctgacgcg gcggcctggc tgcgtgcgca cgacgcggcc gccgcgcccg tggtcgacga
     2281 gctgctccgc tatctcagcg tcgtacagat cggctttccc cggttcgcgc ggcaggacat
     2341 gcagctgctc ggtgccaacg tgcgtgcggg cgatgtggtc atctgttcgc tcagcggcgc
     2401 gaaccgcgac gaggcgtggg caggggcaca cgccgacgca ttcgacccgc atcgcttcga
     2461 cggcgctcgg ccgatgacgt cgcacctggc cttcggctac ggcatgcacc gctgcgtcgg
     2521 cgccgagctc ggccggatgg agctgctcac cgccatcccg gcgctgctgc gacggttccc
     2581 gaagatgtcg ctcgccgtcg gaccgagcaa gctgcggttc cgcgacctgt cgatcgtcta
     2641 cggcatcgac gaactcccgg tgcgcttggg cgactaggac caggcattcg gtggtggttt
     2701 gcctcggtag ccatccaaac ggacgggtag ccaggcaggt ctgccttggg aggggtgggt
     2761 ttgcatggtg accgtggcgg atcggggcgt tgaccggcgg atcttcagca ccaatcggac
     2821 aactgtcggt ggtggtctgc ctcggtagcc atccaaacgg acgggtagcc aggcaggtct
     2881 gccttgggag gggtgggttt gcatgggtag ccatgcaaac cgcgctcggc gaccgtcgta
     2941 tgtccgaaac ccgccagtca cacaggcaaa atgcacggag agccggccga ccggctcagc
     3001 ggccacgaac attcccgctc ttcgttgccg gccggtccca gccaaggctg acagagcgcc
     3061 ggccgaccac gccgaacgaa tggcgaccac ctccgcgcac agaacccgag gagtccccca
     3121 gctccgcctg gcaaccgagc ccttgccacg ggagcaaggc agatcaggcg cgatggccaa
     3181 ggacgtccgg aaccgtgaac cagccggtca gtcgcgggcg cgacgccgga aggcgcggac
     3241 cgcgagtggg gcgaaggcgg ccgtgatgag tacgacccag cagaccgtgg cgatctcggg
     3301 atagcgcaac ggcaacgcgg cgtctgccgg cgcctcgggg ccgttgcccc acaggacccg
     3361 acaggcctgc gtcagggcgg agacggggtt ccactctgcg atcacctgca gccagttcgg
     3421 catgccctcg gtcgggacga acgtgttggc gacgaacgtg agcgggaaca tcagggtgaa
     3481 catgaacccc tggacggcct cgaccgagcg caacgtcgac cctgtccaga tgcccagcca
     3541 gatcatcgag aatccccaca gcagcagcaa tgcgaatgcg agcacggcgt tccagacgcc
     3601 gttgtcgatg cgccaaccga tgagcaggcc ggtcagcgcc atcaccacga tgccgatgct
     3661 tgagtggatg aggctggaga tgctgcgccc gatcagcacg gacgagcggg cgatcggcaa
     3721 ggatcgcatc cggtcgatga tgcccttgtc gaggtcggcc gtcaggccca cggcgacgac
     3781 gaacgacgag aacacgatcg tctgcaccat gatcccgggc agcaggaacg cgttgtagga
     3841 gccaccaccg ggaacgctga tcgctccccc gaagacgtac gcgaacaaca gcacgaacat
     3901 caccggctgg atggtgacgt ccatcaggct ctcgggcatc cgcttgatgt ggatcaggtt
     3961 tcgc
//
LOCUS       DATBFZ010000169         4724 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_3394146, whole genome shotgun sequence.
ACCESSION   DATBFZ010000169 DATBFZ010000000
VERSION     DATBFZ010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 4724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4724
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3394146"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="VH419_05010"
     CDS             complement(<1..261)
                     /locus_tag="VH419_05010"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008361658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEY7043010.1"
                     /translation="MPRQIITTSHAPSSPLFSQGVKAGSQVFISGTTGVDPSTGRMAG
                     DSIQAQTRQALANCEAVLQEAGATLDDVVEVGVLLTDPADFAG"
     gene            complement(329..1051)
                     /locus_tag="VH419_05015"
     CDS             complement(329..1051)
                     /locus_tag="VH419_05015"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008363994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY7043011.1"
                     /translation="MPRHALVTGGGTGLGLAVARRLVADGLQVTIVGRRSEVLDRAAA
                     DLGAAVSTVVADVTDASAIARLADALPERVDVIVHNAGGNSPSTGDTLEDLAHDWLAD
                     YRLNVVSAVLVTEALRSRLPTPGGRIIAMSSVAALRGAGSYGAAKGALNVWVTDLAAQ
                     LAPDGITVNAVAPGFIPETGFWDGRRTPELVEQRTARIPMGRSGTPDEVAALVAHLAS
                     PEAGFTTGQVIGIHGGTVLARL"
     gene            1230..2456
                     /locus_tag="VH419_05020"
     CDS             1230..2456
                     /locus_tag="VH419_05020"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEY7043012.1"
                     /translation="MTRPHVLALIQAGGAGGRMDVLTRERAKPALPFAGVFQLVDFPL
                     SNLANSGITDVWLSVQFQGAGLGEEVANGRPWDLDRNRGGLRLLMPQEGTGATDEEGF
                     ARGNADELYRFRDQVAADAPEVLAVLSADHVYRFDLGEAIDTHRRTGAECTIVTTSIP
                     IGEAGDHATVEADDDGRVTGFAYKPDAPRTGTVSTEIFLYDPEVLVTVLEELHRELGP
                     AAGAGDTGLGDYGEHLIPHLVARGRSFVHELRGYWKDLGQPHKYLAAHHDILTDDLGV
                     LGDVDWPIIGHQQQRPAARILEGSHVSDSLASPGSRVRGTVIRSVLGPGVVIDEGAVV
                     RDSVLFRDTHVRSGARVDWAVVDQDCVIGPRATVGSPDTDPQDPDAIVLIGRGSTVGR
                     DVVLGEGSRLEPGTTA"
     gene            complement(2520..3362)
                     /locus_tag="VH419_05025"
     CDS             complement(2520..3362)
                     /locus_tag="VH419_05025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA family transcriptional regulator"
                     /protein_id="HEY7043013.1"
                     /translation="MTILITGGTGKTGYRVAHRLTGLGHSVRAVTRHSDPPFDWHDDS
                     TWDAAITGCTSAYVTFQPDLALPGADAIIGAFARRAVKLGCTRLVLLSGRGEDGAQRS
                     EAALIAAGADWTILRSAFFFQNFTEAFWAEEIATGSLTMVESTVAEPFVDADDIADVA
                     VATLLDPAHAGRIHELSGPRLITFEGVTNEISMVTGRSLEYQRLPVDDYVAALVSAGF
                     TQEEATGLAYLFDQVLDGRNAHLETGVAEILGREPRDLTAFLNDTLTSRTPSSRDRHV
                     VEQR"
     gene            complement(3442..>4724)
                     /locus_tag="VH419_05030"
     CDS             complement(3442..>4724)
                     /locus_tag="VH419_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043014.1"
                     /translation="PLRTGAGGYAQAGWAFRIQDERNAYVWLLSTQGAGGMAVLKKAV
                     FVGGSPVVTSVPLPYQLEEGKTYHVETTLSGSTFTTRIDGVVVDTTTDTTYAAGSAGF
                     REAGSESAYFDNVTVTGAGGTTLFSDGFDSGLGAWRLPAGTGYGSFAGMHLYDEPSAG
                     QLPLMAKAVAMANDAYPDALSYVNLLPGFGAGYEQAARDLDSAVISFDRYPIVANGED
                     LGYFDNWAQVRAAALKHGRDSWVFIQSVGYANHAIPTKADLLWQISISLAYGCKGIQY
                     FTYWTPDPARGEAFTTGLITVDGKRTRLYRYAKDVNAEYLAPLGKQMLDLTSTAVQVA
                     GVPSPPAGLEPFAEDDLVSEVTGDPVVLGRFVDADDRLYTLVANYSRHERARVRLTLP
                     SDTEAYDPERGRWERQRSGVLALEAGAARLLRAR"
BASE COUNT          712 a         1560 c         1710 g          742 t
ORIGIN      
        1 gccagcgaag tcggcggggt cggtgagcag gacgcctacc tcgacgacgt catcgagcgt
       61 tgcgccggcc tcctgcagga cggcctcgca gttcgccaat gcctggcggg tctgagcctg
      121 aatgctgtcg ccggccatgc gacccgtgct cgggtcgaca ccggtggttc ccgagatgaa
      181 gacctgggat ccggccttga cgccctggct gaagagcggc gagctcggtg cgtgtgacgt
      241 cgtgatgatc tggcgtggca tctcgaacct cccggctgat cgtcccatcg tccgaggtcc
      301 ccgggaaggt gctctccgca cctggcgttc acagccgggc gagaacggtg cccccgtgga
      361 tgccgatcac ctgcccggtc gtgaagccgg cctcgggcga ggcgaggtgg gccaccagcg
      421 ccgcgacctc gtcgggagta cccgagcgtc ccatcgggat ccgggccgtg cgttgctcga
      481 ccagctccgg agtgcgccgg ccgtcccaga agccggtctc ggggatgaag cccggcgcca
      541 cggcgttgac cgtgatgccg tcgggcgcca gctgggcagc gagatcggtc acccagacgt
      601 tgagcgcgcc cttcgcggcg ccgtaggacc ctgccccgcg gagggccgcc accgaggaca
      661 tcgcaatgat gcgaccgccg ggcgtcggca gtcttgacct gagcgcctcc gtcacgagga
      721 ctgcggagac gacgttcagt cggtagtcgg ccagccagtc gtgggccagg tcctcgagcg
      781 tgtcgccggt cgacggggag ttgccgccgg cgttgtggac gatgacgtcg acccgttcgg
      841 gaagcgcgtc cgccaggcgt gcgatcgcgc tcgcgtcggt cacgtctgcg acgacggtcg
      901 agaccgccgc tccgagatcg gcggcggcac ggtcgagcac ctcggaacgc cggccgacga
      961 tggtgacctg cagcccgtcg gcgaccagcc tgcgtgcgac agcgagtccg agaccggttc
     1021 caccgccagt gaccagggca tgacgaggca tgtcccacac gctactgagt cactgaacct
     1081 gccgttcttg ccaccccggt gtcgagaacg catcagcgtc acggtgccgt cctcgcgacc
     1141 cggcatcagc gatcgtctaa ggggggtcgg cgtcgacctc cgatggaccg gcgggtgtgc
     1201 cgttgcgccg gtcagggaac tgagcggaga tgactcgtcc ccatgtgctc gcgttgatcc
     1261 aggccggcgg cgcgggcggg cggatggacg tcctgacgcg ggagcgggcc aagcccgcac
     1321 tgccgttcgc cggggtgttc cagctggtcg acttcccgtt gtcgaatctg gcgaacagcg
     1381 gcatcaccga cgtctggctg tcggtccagt tccagggcgc cgggttgggc gaggaggtgg
     1441 ccaacggccg tccgtgggac ctggaccgca atcgcggggg tctccggttg ctgatgccac
     1501 aggagggcac gggggcaacc gacgaggagg ggttcgccag gggcaacgcg gatgagctct
     1561 atcgcttccg cgaccaggtc gcggccgacg cgcccgaggt gttggccgtg ctcagcgcgg
     1621 atcacgtcta ccgcttcgac ctgggagagg cgatcgacac gcaccgacgt accggcgctg
     1681 agtgcaccat cgtcaccacg tcgatcccga tcggggaggc cggtgaccac gctacggtcg
     1741 aggcggacga cgacggcagg gtcaccggct tcgcgtacaa gccggacgca cccaggaccg
     1801 gaacggtgtc caccgagatc ttcctgtacg accccgaggt actggtgacc gtgctcgagg
     1861 agctccatcg cgagctcggt ccggccgccg gcgctggcga caccggactg ggcgactacg
     1921 gcgaacacct gatcccgcat ctcgtcgcgc ggggtcggtc gttcgtccac gagctgcgcg
     1981 gctattggaa ggatctcggt cagccccaca agtacctcgc cgcacatcac gacatcctca
     2041 ccgacgacct gggagtcctt ggcgacgtgg actggccgat catcggccac caacaacagc
     2101 gacccgccgc caggattctg gaaggcagcc acgtctccga cagcttggcc agcccgggat
     2161 cgcgcgtgcg cgggacggtc atccgaagcg tcctcgggcc cggtgtcgtg atcgacgaag
     2221 gtgcggtcgt gcgcgacagc gttctcttcc gcgacaccca cgtccggtcg ggagcgcggg
     2281 tggactgggc ggtcgtggac caggactgcg tcatcgggcc ccgggccacg gtcggatctc
     2341 cggacaccga tccccaggat ccggacgcga tcgtgctgat cggccgaggg tcgaccgtgg
     2401 gacgcgacgt cgtcctcggc gagggatcgc gcttggagcc cggaaccact gcctgaacgc
     2461 atccggacgg ggaggcggac gctggaggtg cgcgcccagc atttcaggtc cgtctcgcct
     2521 taccgctgct cgacgacgtg ccgatctcgc gatgatggtg tcctcgaggt gagggtgtca
     2581 ttcaggaagg cggtgaggtc ccggggctcg cgcccgagga tctcggcgac gccggtttcg
     2641 agatgggcgt tgcgcccgtc gagtacctgg tcgaacaggt aggccagtcc ggtggcttcc
     2701 tcctgggtga atccggccga gacgagcgcg gcaacgtagt cgtcgacggg tagccgctgg
     2761 tattcgaggg accgcccggt gaccatgctg atctcgttcg tgacgccctc gaacgtgatg
     2821 aggcggggcc cggacagttc gtggatgcgg ccggcgtggg cgggatcgag gagcgtggcg
     2881 acagcgacgt cggcgatgtc atcggcgtcg acgaacggct cggcgaccgt gctctcgacc
     2941 atcgtgaggg agccggtcgc gatctcctcg gcccagaagg cctcggtgaa gttctggaag
     3001 aagaacgccg agcgcaggat cgtccagtcg gcgccggcgg cgatgagggc ggcctccgac
     3061 cgctgcgccc cgtcctcacc gcgtcctgac aggagtacga gacgggtgca cccgagcttg
     3121 acggcgcggc gggcgaaagc gccgatgatg gcgtctgcgc cagggagagc gagatcgggc
     3181 tggaaggtga cgtaggccga cgtgcatccg gtgatcgcgg catcccaggt cgagtcgtcg
     3241 tgccagtcga acggggggtc gctgtggcgg gtgacggcgc ggacggagtg gccgagtccg
     3301 gtcaggcgat gtgcgacgcg gtagccggtc ttgccggttc cgccggtgat gagaatggtc
     3361 atgcacccaa gactgggtgg ctcggtggac gctgagaatg tgtatcacgc tcgtaaacat
     3421 gcgccagcgt ctctcaagtg ctcaccgcgc ccgcagcagc cgcgccgccc ctgcttccag
     3481 cgccagcacg ccgcttcgct ggcgctccca gcggccgcgc tcgggatcgt atgcctccgt
     3541 gtccgacggc agggtcaggc gtacgcgggc ccgctcgtgg cgtgagtagt tcgcgaccaa
     3601 tgtgtacagg cggtcgtcgg cgtcgacgaa gcgcccgagg acgaccgggt cgccggtgac
     3661 ctccgacacc aggtcgtcct cggcgaacgg ttcgagtccc gcgggcggac tgggcacgcc
     3721 cgcgacctgg acggcggtcg aggtgaggtc gagcatctgc ttgcctagcg gcgcgaggta
     3781 ctcggcgttg acgtccttcg cgtaccggta gagcctggtc cgcttgccgt cgaccgtgat
     3841 cagaccggtc gtgaacgctt cgccgcgggc cgggtcgggc gtccagtagg tgaagtactg
     3901 gatgcccttg cacccgtacg cgaggctgat cgaaatctgc cagagcaggt ccgccttggt
     3961 cgggatcgcg tggttcgcgt agccgacgct ctggatgaag acccaggagt cgcgcccgtg
     4021 cttcagcgcg gcagcccgga cttgcgccca gttgtcgaag tagccgaggt cctccccgtt
     4081 ggccacgatc ggatagcggt cgaaggagat gaccgccgag tccaggtcgc gcgccgcctg
     4141 ctcgtagccg gcgccgaagc ccggcaggag gttgacgtag gagagcgcat ccggataggc
     4201 gtcattggcc atcgcgacgg ccttcgccat cagcgggagc tggccggcgc tcggctcgtc
     4261 gtacaggtgc atgccggcga acgacccgta tccggtcccc gcgggcagcc gccaggcgcc
     4321 gagtccggag tcgaagccgt cgctgaacag cgtcgtgccg ccggcacccg tcacggtgac
     4381 gttgtcgaag tacgccgact ccgaccccgc ctcgcggaat cccgccgagc ccgcggcgta
     4441 cgtcgtgtcg gtcgtggtgt cgaccacgac tccgtcgatc cgggtggtga acgtggagcc
     4501 gctcagcgtc gtctcgacgt ggtaggtctt cccctcctcg agctggtacg gcagcgggac
     4561 cgacgtgacg acggggctgc cgccgacgaa caccgccttc ttgagcaccg ccatgccgcc
     4621 tgccccctgg gtggagagca gccagacgta cgcgttgcgc tcgtcctgga tcctgaacgc
     4681 ccaacccgcc tgggcgtatc cgccggcgcc ggtgcgcagc ggct
//
LOCUS       DATBFZ010000170         9951 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_3394632, whole genome shotgun sequence.
ACCESSION   DATBFZ010000170 DATBFZ010000000
VERSION     DATBFZ010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 9951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9951
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3394632"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..549
                     /gene="dhaL"
                     /locus_tag="VH419_05035"
     CDS             <1..549
                     /gene="dhaL"
                     /locus_tag="VH419_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002520112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HEY7043015.1"
                     /translation="ELDSAIGDADHGINMDRGMKAAVAAVGDGSAETADALLKKVGMT
                     LVSTVGGASGPLYGTFFLRCGTALTDVDDIDAAAFGVALRAGVDGLVARGKAELEDKT
                     MYDAWAPALEAYDAKVSDGGAIGDALRAAADAAAEGRDATTPMVARKGRASYLGERSA
                     GHQDPGATSTTMLLEAAAGTLS"
     gene            586..990
                     /gene="dhaM"
                     /locus_tag="VH419_05040"
                     /pseudo
     CDS             586..990
                     /gene="dhaM"
                     /locus_tag="VH419_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002514950.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
     gene            1084..3042
                     /gene="ptsP"
                     /locus_tag="VH419_05045"
     CDS             1084..3042
                     /gene="ptsP"
                     /locus_tag="VH419_05045"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEY7043016.1"
                     /translation="MTVSNAHGLHARPAAKVVALTRGFDAHVTLTNLDTGRGPVNAAS
                     LSAVATLDARQGHRLAVAASGPQAAEAVQALESLAERGFGDTPAATAAVTPRAAGSGL
                     DLAMGPALVRRSTVDVSAYEPGDVDEERARSGKAVEAVRRRLLELERRTTEESGAEAG
                     AIFAAHQTLLEDPEVIEQVTADIRSGRSAVEAWRGRLDAVADGFEQLADPYQRERAAD
                     VRSVQVALLRELTGGSEPEESDESRPAVLVVDELDPATAASIDVERVAGVAVVARGDT
                     GHGVIIASSRGIPLITGVGRDADGVRTGQPVAFDARRGRFWSTVTDEDQRRWPEYVEQ
                     RTRERAALVETAQQPATTSDGRRIPVLANVASVADAESAYANGADGSGLVRTEVLFGD
                     CETAPSVEEQTETFLAVADALGGRQLTIRTWDIGGDKPLPFLPMPREANPFLGVRGIR
                     AFDPPIAESANRLLTDQLTAICRAAHRTPIRVMFPMVTTRHEVTDAMMALRSAARGEP
                     PDGLEVGIMVEVPAAAVMIDRLAVGLDFVSIGTNDLTQYTVAADRGNDAVADLADPLS
                     PAVLRLIHQVCRTRPDGVEVAVCGDLASRPEIAGLLIGLGVDELSCTPPKVPDVKAAV
                     RRTSLADARRLAADVLDADEPAVIRERL"
     gene            complement(3054..3923)
                     /locus_tag="VH419_05050"
     CDS             complement(3054..3923)
                     /locus_tag="VH419_05050"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY7043017.1"
                     /translation="MSGAVGSAVDSGEDVWRALSNPVRRHLLDLMSRGPQTTSELALA
                     FPELSRFAVMQHLGVLTDAGLIVVRRRGRHRFNHLNPVPLRRWYERWVVPLADRSAAE
                     LLALKTNLETGIDSPNGDTSMSAITDATTDASPDASPDASTDELRTVRIEAELRFHAS
                     PDRVFQALTEETRSWFPHTYGGDRVQAIVMEPRVGGAHFEDWGDGMGHLYGHVTVYDR
                     PYRLCLRGRIMPGSMLDTEYTLEPDGDQGDQTVLRMSKVAVGPMTADEADGIRTFGDI
                     SRFEDALRALVEA"
     gene            4041..4457
                     /gene="tsaA"
                     /locus_tag="VH419_05055"
     CDS             4041..4457
                     /gene="tsaA"
                     /locus_tag="VH419_05055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HEY7043018.1"
                     /translation="MTHERYDVRVIGRVESPLGDREEAPNQGNEGAPEAWLTFEPHVS
                     AAMADLQVGEDVIVLTWLDRADRNVLQTHPRGDETIPLHGVFSTRSPDRPNPIGLHRV
                     RILEIAGTRIRVSALEALDGTPIVDVKPVLDRSLER"
     gene            complement(4536..5945)
                     /locus_tag="VH419_05060"
     CDS             complement(4536..5945)
                     /locus_tag="VH419_05060"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008363267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY7043019.1"
                     /translation="MTASNLTGSPTYADLSLWHATCGDPLLPRPGLPGDLDVDVAIVG
                     AGFTGLWTAYYLAAADPGLRIAVIERDIAGFGASGRNGGWASALFPTSRERLAGIPGS
                     SRERANAQIAAMRASIGEIERVLTIESLDCDFHRGGTVVFARTPVQLERARAEVDDAR
                     AWGDTEADLRLLSAAETTEMVAVSGALGATYTPHCARIHPAKLARGLAAAVERRGVAL
                     YEQTEVTAIAPQRVSTQGGSVRASYVLRATEGYTARLPGLRRSVAPVYSLVVATEPLP
                     DDLWAHVGLTDAPTFSDERHLIIYGQRTADGRIVFGGRGAPYHFASSVAPEHDHEGRV
                     FADLRSTLVELFPMLRDVRFSHAWGGPLGVPRDWMASVGLDRSTGLGWAGGYVGDGVT
                     TTNLAGRTLADLVRGEESDLTRLPWVNHRSRRWEPEPLRWLGVNAGLRAMALADAEER
                     RTGKPSRIARAMSPLLGGH"
     gene            complement(5942..6415)
                     /locus_tag="VH419_05065"
     CDS             complement(5942..6415)
                     /locus_tag="VH419_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008363265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEY7043020.1"
                     /translation="MAQRNDGTAPILDDVSKGIIEQLQRDGRMPYAAIGKAVGLSEAA
                     VRQRVQRLLDSGVMQIVAVTDPLQLGFARQAMIGIHAEGDLDPIADALAAMTEVDYVI
                     VTAGSFDLIVEVVCESDDHLLELLTKRIRALDGVRSTETFVYLKLHKQTYSWGVR"
     gene            6564..7736
                     /locus_tag="VH419_05070"
     CDS             6564..7736
                     /locus_tag="VH419_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEY7043021.1"
                     /translation="MPVLGMSQRPIPRRTFLRGASLTAMALAVPGLSACGTEGAKIEP
                     GSCTSLDQSATDKSLVFSNWPEYIDPISKKNSSLSRFEEQTGISVDYIPDVNDNETFF
                     AKVADQLADCVSTGRDIFTLTDWMAAKMIELGWIQKLDKSKLPNVDANILDSLASPGW
                     DPKREYSVPWQSGMTGICYNKELTDPVGSFEELLTRSDLKGKVDLLSEMRDTMLFMLL
                     LVGANPEDFTFAEFESAIELLEKAVSDGQIRRFTGNDYTEDLRGGNVVACEAWSGDIP
                     QLGDPKFVWVPAEEGVSLWSDNMIVPNKADHRTNAEELMNFYYDPENAAQLSAWNYYF
                     CPVQGTEDYIAEFDKSAVGNPLIFPTEKYLKNGYNFMALGDKEREYTALFSEVMTG"
     gene            7733..8899
                     /locus_tag="VH419_05075"
     CDS             7733..8899
                     /locus_tag="VH419_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017933992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY7043022.1"
                     /translation="MTTTATVSSDDVGQRSDEDLRLVNVTKQFGTFTAVNDLTLTVPS
                     GSFFALLGPSGCGKTTTLRMVAGLEDPTTGELYIGDQEISRLKPYKRPVNTVFQSYAL
                     FPHLTIFENVAFGLRRRGRKDVKSAVNEMLELVELSEFAGRKPAQLSGGQQQRVALAR
                     ALINEPGVLLLDEPLGALDLKLRRQMQIELKRIQTEVGITFVHVTHDQEEAMTMADTI
                     AVMNGGVIDQMGAPEDLYENPSTTFVANFLGQSNLVMTDVLSQGDTHTQINCNGEKLV
                     VPKDRDHSGGGRGWLGVRPEKIFASTPGGEGHAESNVLTGGHVSDVSFIGVSTQYLVR
                     MPWGQELMVFEQNTGQRERFAQGDPVDVLWNPAHSFLLDPDQDAEAGVTREEDL"
     gene            8896..9792
                     /locus_tag="VH419_05080"
     CDS             8896..9792
                     /locus_tag="VH419_05080"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY7043023.1"
                     /translation="MSVAPVASQQPVTDGETTRKRGWGWLLIIPGMLWLAIFFLFPTI
                     QLLGTSLYDPNGSFEFGYNMTWHFQNYVDAIQDNAKFFVRSFAYAGIATLTCLLLGYP
                     LAYAIAFKSGRWKNFMLVLVIAPFFTSFLLRTITWQSILSDQGWVVDTLKWLHILGPD
                     GRLLATSFAVVAGLTYNFLPFMTLPIFASLDKIDPRLIEASSDLYANPFTGLRKVTFP
                     LSMPGVVSGTLLTFIPACGDYINAELLGTPQQFMIGNKIQDLYLRQKDYPAAATLSII
                     LMVIVLVMVLFYIRRAGTEELV"
BASE COUNT         1651 a         3343 c         3390 g         1567 t
ORIGIN      
        1 gagctcgact ccgcgatcgg cgacgccgac cacggcatca acatggaccg cgggatgaag
       61 gcggccgtgg ccgcggtcgg ggacggatcg gccgagacgg ctgacgcgtt gctgaagaag
      121 gtcggcatga ctctggtcag caccgtcggc ggcgccagcg gtccgctgta cggcacgttc
      181 ttcctccgct gcgggaccgc gctcaccgac gtcgacgaca tcgacgcggc cgcgttcggt
      241 gtggcgctgc gcgcgggcgt cgacgggctc gtcgcccgcg gcaaggccga gctcgaggac
      301 aagacgatgt acgacgcgtg ggctccggcg ctcgaggcgt acgacgcgaa ggtgtcggac
      361 ggcggcgcga tcggtgacgc cttgcgagcc gctgccgacg cggccgcgga gggcagggac
      421 gcgacgacgc cgatggtggc tcgcaaaggg cgggcgagct acctcggcga acgcagtgcc
      481 ggccatcagg atcccggggc gacgagcacg acgatgttgc tggaggccgc ggccggcacg
      541 ctgtcgtgac cggcgcggac agggatcgcc gcgaatgacc acggcatggt cggccggtgg
      601 tcggccggtg atcggcccat gatcggcatc gtcgtcgtct cgcacagccg gtcgctcgcg
      661 gacgctgcgg tcgccctggc gagcgagatg gtggacgcga agtcggcgcc ggcgatcgcg
      721 gtcgctgccg gtctggacga gacgaccttc gggacggacg cgactgctgt ctccgcggcg
      781 atcgagcagg tggactcacc cgacggcgta ctcgtgctgc tcgacctcgg tagtgcgctg
      841 ctgagtgcgg agatggctct ggagttcgtc gatcccgact tggtcgctcg ggtgcggctg
      901 tcgagcgctc cactggtcga gggcctggtc gctgccgtcg tcctcgccgc caccggtgcc
      961 gacctggacg ccgtcgccgg ggaggccgag aacggcctgc tcgcgaagca gtcgcacctc
     1021 ggcccggcag actcggcgtc gcgcggtgac gagcgtgttg ctgcgcccga cgtcgccggt
     1081 gaggtgacgg tctcgaacgc ccatggcctg cacgccaggc cggcggcgaa ggtggtggcg
     1141 ctcacccggg gcttcgacgc ccacgtgacc ttgaccaacc tcgacacggg ccgcgggcct
     1201 gtgaacgccg ccagcctcag cgctgtcgcg acgttggacg cccgccaggg acaccgcctt
     1261 gccgtggcag cctccggacc gcaggcggcc gaggcggtgc aagcgctgga atcgctggcc
     1321 gagcggggtt tcggcgacac gcctgcagct acggctgctg tcacgccccg cgctgccgga
     1381 agcgggctcg acctcgcgat gggtcctgca ttggtacgcc ggtcgacggt cgacgtgtcc
     1441 gcgtacgagc ccggagatgt cgacgaggag agggctcgtt ccgggaaggc ggtcgaagcg
     1501 gtccggcggc ggctgctcga gctcgaacgg cgtacgacgg aggagtccgg cgccgaagcc
     1561 ggagccatct ttgcggcgca ccagacgttg ctcgaggatc cggaggtgat cgagcaggtc
     1621 accgcggaca tcaggtccgg ccggtcggcc gtcgaggcgt ggcgaggacg tctcgatgcg
     1681 gtggctgacg ggttcgagca gctggccgat ccctaccagc gcgagcgggc cgccgacgtc
     1741 cgcagcgtgc aggtcgcgct gctccgcgag ctgacgggcg ggtccgagcc cgaggagagc
     1801 gacgaaagtc gaccggccgt cctcgtcgtg gacgagctcg acccggccac cgctgcgtcg
     1861 atcgacgtcg agcgggtcgc cggggtcgcg gtggtggcgc gaggcgacac cggtcacggc
     1921 gtgatcatcg cctcatcgcg cggcatcccg ctcatcaccg gtgtcggacg tgacgcggac
     1981 ggcgtacgta ccggtcagcc ggtggcgttc gatgccagac gcggcaggtt ctggtcgacg
     2041 gtcaccgacg aggaccagcg gcgatggccc gagtacgtcg agcagcgcac gcgcgaacgc
     2101 gcagcgctgg tcgagaccgc gcagcaacct gccacgacct ccgacgggcg gcggattccg
     2161 gtgctggcga acgtcgcttc ggtcgccgac gcggagtcgg cgtacgccaa cggcgccgac
     2221 ggttcagggc tggtgcggac cgaggtgctg ttcggcgact gtgagacggc gccgtcggtc
     2281 gaggagcaga ccgagacctt tctcgcggtg gcggacgcgc tcggcggacg acagctgacg
     2341 atccggacct gggacatcgg gggagacaaa ccactgccgt tcctgccgat gccgcgcgag
     2401 gcgaacccgt tcctcggcgt tcgcgggatc cgcgctttcg atcctccgat cgcggagtcg
     2461 gccaaccgac tgctcacgga ccagttgacg gcgatctgcc gggcggcgca ccggacgccg
     2521 atccgggtga tgttcccgat ggtcacgacg cgccacgagg tcaccgacgc gatgatggcc
     2581 ctgcgttcgg cggcccgtgg tgagccgccg gacgggctcg aggtcgggat catggtcgag
     2641 gttccggcgg cggcggtcat gatcgaccgg ttggcggtcg gcctcgactt cgtgagcatc
     2701 ggcacgaacg acctcaccca gtacacggtc gccgccgacc ggggcaacga cgcagtcgcc
     2761 gacctcgccg acccgctcag cccggccgtg ctgcgactga ttcatcaggt gtgccggacg
     2821 cgtccggacg gcgtcgaggt cgcggtgtgt ggcgacctgg cgagccgtcc cgagatcgcc
     2881 ggtctcctga tcgggctcgg cgtcgacgag ctgagctgta cgccgccgaa ggtgcccgac
     2941 gtgaaggcgg ccgtgcggcg tacgagcctg gccgacgccc ggcggctcgc cgccgacgtc
     3001 ctcgacgccg acgaaccagc cgtcatccgt gagcggctct gaggagccgg cgctcacgct
     3061 tcgacgagcg cacgcagcgc gtcctcgaag cggctgatgt cgccgaaggt gcgaatgccg
     3121 tccgcctcgt cagccgtcat cggaccgact gcgaccttcg acatcctcag cactgtctgg
     3181 tcaccctggt caccgtccgg ctcgagggtg tactcggtgt ccagcatgct gcccggcatg
     3241 atgcgcccgc gcaggcacag ccggtacggg cggtcgtaga cggtcacgtg gccgtacaga
     3301 tgacccatcc cgtctcccca gtcctcgaag tgcgcgccgc cgacacgcgg ctccatcacg
     3361 atggcctgaa cccggtctcc gccatacgtg tgggggaacc aggatcgtgt ctcctcggtc
     3421 agcgcctgga acacccgatc gggcgaggcg tggaagcgca gttcggcttc gatgcggacg
     3481 gtgcgcagct cgtcggtgct ggcatcgggg ctggcatcgg ggctggcatc ggtggtggca
     3541 tctgtgatgg cggacatgga cgtgtctccg ttcggcgagt cgataccggt ctcgaggttg
     3601 gtcttcaggg cgagcagttc cgcagcagag cgatcggcga gcgggacgac ccaccgttcg
     3661 taccaacggc gcagtgggac cgggttgagg tggttgaacc ggtgccgtcc tcgacggcgt
     3721 acgacgatga gcccggcatc ggtgagcacc ccgaggtgct gcatcacggc gaaccggctg
     3781 agctccggga acgccaaggc cagttcactg gtcgtctgtg gcccgcggct catcaggtcg
     3841 agcaggtggc ggcggaccgg gttggacagc gcccgccaca cgtcctcgcc ggagtccacc
     3901 gcagacccca ccgcaccgga catgtgatat ttctatcaca tgtccggccg aggtggcagg
     3961 ggtgagaccc cacgtttcgt tgggttgtga cggcgtgtcg aacggcgatt gccacctcga
     4021 caccgcgaag agttttcgac atgacgcatg agcggtacga cgtacgagtc atcggtcgag
     4081 tggaatcacc actcggcgac cgagaggagg cgcccaatca ggggaacgaa ggagccccgg
     4141 aggcatggct gaccttcgaa ccgcacgtgt ccgcagcgat ggcggacctg caggttggcg
     4201 aggacgtcat cgtgctcacc tggctggacc gtgccgatcg gaacgtcttg cagacgcatc
     4261 cacgcgggga cgagacgatc ccactgcacg gcgtgttcag caccaggtct ccggaccggc
     4321 cgaacccgat cgggctccac cgggtgcgca tcctcgagat cgccgggact cggatcaggg
     4381 tcagcgccct cgaggcgctc gacggcaccc cgatcgtgga cgtcaagcca gtgctcgacc
     4441 gatcgctcga gcggtgaggt ggcaatcgtc gttcgacacg ccgtcagaat gcaacggaac
     4501 ctgccacctc ggcgggaagc ggaagatggg tcaggtcagt ggccgccgag gaggggggac
     4561 atggcgcggg cgatgcggga gggctttccc gtacgccgct cttcggcgtc ggccagcgcc
     4621 atcgcccgta gtccggcgtt gacaccgagc cagcgcagcg gctcgggctc ccaacgtctc
     4681 gaccggtggt tgacccacgg caaccgggtc aggtcggact cctcgccacg caccagatcc
     4741 gccagcgtcc ggccggcgag gttcgtggtc gtgacgccgt cgccgacgta accgccggcc
     4801 cagcccaaac cggtcgaccg gtccaggccg accgaggcca tccagtcgcg cggtacgccg
     4861 agcggtccgc cccatgcgtg cgagaagcgg acgtctctca gcatcgggaa cagctcgacc
     4921 agggtgctcc gcaggtcggc gaaaacccgc ccctcgtggt cgtgctcggg cgccaccgac
     4981 gacgcgaagt ggtacggagc cccccgtccg ccgaagacga tgcgcccgtc agccgtccgc
     5041 tggccgtaga tgatcaggtg gcgttcgtcg gagaacgtcg gtgcgtcggt gagcccgacg
     5101 tgcgcccaca ggtcgtcggg gaggggctcg gtcgccacca ccagcgagta caccggggcg
     5161 accgaccgcc gcagccccgg cagccgtgcc gtgtagccct cggtcgcgcg gagcacgtac
     5221 gacgcccgga ccgatccgcc ctgggtggac acccgctgcg gagcgatcgc ggtgacctcg
     5281 gtctgctcgt agagcgcgac accccgacgc tccaccgccg ccgccagccc gcgggcgagc
     5341 ttcgcagggt ggatgcgcgc gcagtgcggc gtgtacgtgg ccccgagcgc acccgacact
     5401 gcgaccatct ccgtcgtctc ggccgccgag agcagccgga ggtccgcttc ggtgtcgccc
     5461 cacgcacgcg cgtcgtcgac ctctgcacga gcgcgctcga gctggacagg cgtcctggcg
     5521 aagacgacgg tcccgccgcg gtggaagtcg cagtcgagag actcgatggt cagcacccgc
     5581 tcgatctcgc cgatgctggc ccgcatcgca gcgatctgcg cgttcgcacg ctcacgcgac
     5641 gagccaggga taccggcgag cctctcccgg gacgtcggga acaatgccga cgcccatcca
     5701 ccgttgcgtc ccgatgcccc gaagcctgcg atgtcgcgtt cgatcacggc gatccgcagg
     5761 ccaggatcgg cggcggccag atagtacgcc gtccagagtc ccgtgaagcc ggccccgacg
     5821 atcgcgacgt ccacgtcgag gtcgcccgga agccccggcc gggggagcag cgggtcaccg
     5881 caggtggcgt gccagagtga caggtcggca tacgtcggtg aaccggtgag gttcgacgcc
     5941 gtcaccggac tccccacgag taggtctgtt tgtgcagctt caggtacacg aaggtctccg
     6001 tgctccggac gccatcgagg gcgcggatcc gcttcgtcag cagttccagc agatggtcgt
     6061 cgctctcgca gacgacctcg acgatcaggt cgaacgaccc ggcggtcacg atgacgtagt
     6121 cgacctctgt catggcggcc aacgcgtcgg cgatcgggtc gagatcgccc tccgcgtgga
     6181 tgccgatcat cgcctgcctg gcgaagccga gctgcaatgg gtcggtgacg gccacgatct
     6241 gcatcacgcc gctgtcgagc aaccgttgca cccgttgacg gactgctgcc tccgacaggc
     6301 cgactgcctt gccgatcgcg gcgtacggca tccgtccgtc gcgttgtagc tgctcgatga
     6361 tgccctttga gacgtcgtcc agaatcggcg ccgtaccatc attgcgttga gccacgcagt
     6421 catcttggtg gcgataccca gggtagtgca agcattacgc cgcagattcc gtcgtcagga
     6481 gccgattcct cgacggaatc ccttgtatcg atccgtacgc tctgacaaga tcgcctcgtc
     6541 cgggctggaa agaagggtga tagatgccag tcctcggtat gtcgcagcgg ccgatcccgc
     6601 gccgcacatt tctccgcgga gcttcgctga cggcgatggc gctcgcggtt cccggactga
     6661 gtgcctgcgg gacagagggc gccaagatcg agccggggtc gtgcacgagc ctggaccagt
     6721 ccgcgacgga caaatcgttg gtgttctcga actggccgga gtacatcgac ccgatcagca
     6781 agaagaactc ctccctgtcg aggttcgagg agcagacggg gatctcggtc gactacatcc
     6841 cggacgtcaa cgacaacgag accttcttcg ccaaggttgc cgaccagctt gcggactgcg
     6901 tctccaccgg ccgcgacatc ttcacgctga ccgactggat ggccgcgaag atgatcgagc
     6961 tgggctggat ccagaagctc gacaagtcca agctccccaa cgtcgacgcc aacatcctcg
     7021 actcgctggc ctcgccgggg tgggatccca agcgcgagta cagcgtcccg tggcagagcg
     7081 ggatgaccgg catctgctac aacaaggagc tcaccgaccc ggtcggcagt ttcgaggagc
     7141 tcctgacccg ctccgacctg aagggcaagg tcgacctgct gagcgagatg cgcgacacga
     7201 tgctcttcat gttgctcctc gtcggcgcca accccgagga cttcaccttc gccgagttcg
     7261 agagcgcgat cgagctgttg gagaaagccg tctccgacgg acagatccgc cgcttcaccg
     7321 gcaacgacta cacggaggac ctccgcggtg ggaacgtcgt cgcctgcgag gcatggagcg
     7381 gcgacatccc acagctcggc gaccccaagt tcgtctgggt cccggcagag gagggcgtct
     7441 cactgtggag cgacaacatg atcgtcccca acaaggccga ccaccggacg aacgccgaag
     7501 agctgatgaa cttctactac gacccggaga acgccgccca gctctcggcc tggaactact
     7561 acttctgccc ggtccagggg accgaggact acatcgccga gttcgacaag tcggcggtgg
     7621 gcaacccgtt gatcttcccc acagagaagt acctgaagaa cggctacaac ttcatggcac
     7681 tcggcgacaa ggagcgtgaa tacaccgctc tcttctcgga ggtgatgacc ggatgacgac
     7741 cacagcgacc gtcagcagcg acgacgtcgg gcagcgaagc gacgaggacc tccgactggt
     7801 caacgtcacg aaacagttcg ggaccttcac cgcggtcaac gacctcacgc tcacggtccc
     7861 gtccggatcg ttcttcgccc tgctcgggcc gtccgggtgc ggcaagacca ccacgttgcg
     7921 catggtggcc gggctcgagg acccgacgac gggcgagctg tatatcggcg accaggagat
     7981 ctcccgcctc aagccctaca agcgtcccgt caacaccgtc ttccagtcct acgcgctgtt
     8041 cccgcacctg acgatcttcg agaacgtcgc gttcggcctt cggcggcgcg gccgcaagga
     8101 cgtcaagagc gcggtcaacg agatgctcga gctcgtcgag ctgtctgagt tcgccgggcg
     8161 caagccggct cagctgtccg gtgggcagca gcagcgggtt gcgctcgccc gggcgctgat
     8221 caacgaaccc ggcgtgctgc tgctcgacga accgctcggt gcgctggacc tcaagctgcg
     8281 tcggcagatg cagatcgagc tcaagcggat ccagaccgag gtcggcatca ccttcgtcca
     8341 cgtcacgcac gaccaggaag aggccatgac gatggccgac accatcgccg tgatgaacgg
     8401 cggcgtcatc gaccagatgg gcgcgcccga ggatctgtac gagaacccca gtacgacctt
     8461 cgtcgccaac ttcctcggtc agtccaacct cgtcatgacc gacgtgctca gccaaggtga
     8521 cacccacacg cagatcaact gcaacggcga gaagctcgtc gtgccgaagg accgggacca
     8581 cagtggtggt ggccgcggct ggctcggcgt acgaccggag aagatcttcg cgtcgacgcc
     8641 cggtggagag ggacacgccg agtcgaacgt cctcactggt gggcacgtga gcgacgtcag
     8701 cttcatcggg gtcagcaccc agtacctggt gcggatgccc tggggtcagg agctgatggt
     8761 cttcgagcag aacaccggac agcgtgaacg gttcgcccag ggtgatccgg tcgacgtgct
     8821 gtggaacccc gcgcactcct tcctcctcga ccccgaccag gatgccgagg ccggcgtcac
     8881 acgtgaggaa gacctgtgag cgtcgcgccg gtcgcgagcc agcagccggt caccgacgga
     8941 gagaccaccc gcaagcgtgg ttggggctgg ctgctgatca tccccgggat gctgtggctg
     9001 gcgatcttct tcctgttccc gaccatccag ctgctgggca cgagcctgta cgacccgaac
     9061 ggctcgttcg agttcggcta caacatgacg tggcacttcc agaactacgt cgacgcgatc
     9121 caggacaacg ccaagttctt cgtccggtcg ttcgcgtacg ccggaatcgc caccctgacg
     9181 tgtctcctgc tgggctaccc gctcgcctac gcgatcgcgt tcaagtcggg tcgctggaag
     9241 aacttcatgc tggtgctggt catcgctccg ttcttcacca gcttcctgct caggaccatc
     9301 acctggcagt cgatcctgtc cgaccaggga tgggtggtcg acaccctcaa gtggttgcac
     9361 atcctcggac ctgacggccg tctcctggcg acgtcgttcg ccgtcgtcgc cggactgacc
     9421 tacaacttcc tgccgttcat gacgctgccg atcttcgcga gcctcgacaa gatcgacccg
     9481 cgtctgatcg aggcatcgtc cgacctctac gccaacccgt tcaccggact gcggaaggtg
     9541 accttcccgc tgtccatgcc cggtgtcgtg tccggcacgc tcctcacgtt catcccggcg
     9601 tgtggcgact acatcaacgc tgagctgctc ggcacaccgc agcagttcat gatcggcaac
     9661 aagatccagg acctctacct gcggcagaag gactacccgg ccgcggcgac cttgtcgatc
     9721 atcttgatgg tgatcgtctt ggtgatggtg ctcttctaca tccggcgcgc agggacggag
     9781 gagctggtct gatggcgacc gcaacccctg ccgcgatcgc cacgagcgtc gagacgccga
     9841 ggcgctctcg gcccgggcgt cgaggcttca tcgggtggtt cagggacaac atcatcctgc
     9901 tcatcgggat cggcgtcctg atctacacgt tcgttcccat cgcatacgtc a
//
LOCUS       DATBFZ010000171         1891 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_3068401, whole genome shotgun sequence.
ACCESSION   DATBFZ010000171 DATBFZ010000000
VERSION     DATBFZ010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 1891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1891
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3068401"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            209..1255
                     /locus_tag="VH419_05085"
     CDS             209..1255
                     /locus_tag="VH419_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043024.1"
                     /translation="MPRHHQLRVLLLAPIAILATVVLAWVIPPRPRTPWPAGTEAAAA
                     LAPAAPVTTSAANQGVGVVAPTTTAPPPPTVPPTTAAVPPPPPPPAPVLPTGKGMWLY
                     LPDEVEGGDVNAIVARAQATGLTHLYVRTGSSVDGFYAAPFLDALLPVAHAGGLKIIG
                     WDFPYFTDIGADISRAVAAIAYTTPTGDRLDGFSPDIETRYEGVWLSPENATLYGAGL
                     RGAVGPSYPLIATVPRPSPSRQADYPYTEVTAAFDAIAPMVYWIDTPPDEDAAQAIDF
                     LSVFGKPVYPIGQAYDGGLEGGPPGTPTGNEIDAFINTSAAHGAKGVSFWSWQHASPE
                     MWNAIANGLEVGAG"
     gene            1287..>1891
                     /locus_tag="VH419_05090"
     CDS             1287..>1891
                     /locus_tag="VH419_05090"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEY7043025.1"
                     /translation="MSVRGRDVLLEVLRTEGVRHIFGNPGSTELPLIDALATVDDPQY
                     VLALQEATAVGMADGYAQATGRPAFLNLHTSAGLGNAIGNLTNARACLTPLVVTAGQQ
                     DYRHIVTDPLLAGDLTGIARAVSKWTHEVRTTDELGTVLRRAFHDAMSPPAGPVFVSI
                     PMDLLDASTDAPVPSPSAIHRRSVADGLDELASLLTERPIGT"
BASE COUNT          297 a          709 c          594 g          291 t
ORIGIN      
        1 ctgacgaccg tcactgcctc acctgggccg atgcgccagg gtgactaccg accaccctga
       61 gtggccgctg agtgatcttg cgcgcgctgc cgcgaaccgg cacactcggc agttccgacg
      121 ccagcggacg cgtcgggccc cataagtacc tagggcgatt ttctctgcgc gggcagcgct
      181 tgtgccggag tcctcgtcac gaaagcgcat gcctcgacac catcaacttc gcgttctcct
      241 gctcgcaccg atcgccatcc tcgccaccgt cgtcctcgcc tgggtcatcc ctccgagacc
      301 gcgtacgccg tggccggccg ggaccgaagc cgcggccgcg ctcgccccgg ccgctcccgt
      361 gacaacgagc gctgccaacc agggcgtcgg cgtcgtcgca ccgacgacca ccgcgccgcc
      421 acctccgacc gtcccaccga cgaccgccgc ggtgcctccg ccaccgccac ctccggcacc
      481 cgtactgccc accggcaagg ggatgtggct gtacctgccc gacgaggtcg agggcggcga
      541 cgtcaacgcg atcgtcgcga gagcacaggc aacaggactc acgcacctct atgtccgcac
      601 cggctcctcg gtcgacgggt tctatgccgc gccgttcctc gacgcgctct tgccggttgc
      661 tcatgccggc gggctgaaga tcatcggttg ggacttcccc tacttcacgg acatcggcgc
      721 cgacatctcc cgcgcggttg cggccatcgc ctacaccaca ccgaccggcg accgcctcga
      781 cggattcagt cctgacatcg agacccgcta cgagggcgtg tggctgtcac cggagaacgc
      841 caccctgtac ggcgccggac tccgtggtgc cgtcggtccg agctatccgc tgatcgcgac
      901 cgtcccccga cccagcccgt ctcggcaggc ggactatccg tacacggaag tcaccgccgc
      961 cttcgatgcg atcgcgccga tggtgtactg gatcgacacg ccgcccgacg aggacgccgc
     1021 gcaagcgatc gacttcctgt ccgtgttcgg caagccggtg tacccgatag gccaggcgta
     1081 cgacggcggg ctcgagggcg gtccgccggg aacgcccacc ggcaacgaga tcgatgcgtt
     1141 catcaacacg tcggctgcgc acggcgccaa aggggtgtcg ttctggtcgt ggcaacacgc
     1201 gagccctgag atgtggaacg cgatcgcgaa cggactcgag gtcggcgccg ggtaaccccg
     1261 ggcgacgcgc ctaacgttgc gacccggtga gtgttcgcgg tcgcgacgtc ctgctggagg
     1321 tgctccggac cgagggtgtc cgtcacatct tcggcaatcc gggcagcacg gagttgccgc
     1381 tcatcgacgc actcgcgacc gtcgacgatc cgcagtacgt gctcgcgctg caagaggcga
     1441 cagcggtcgg catggcagac ggctacgcac aggcgaccgg ccgccccgcg ttcttgaacc
     1501 tgcacacgtc tgccggcctc ggcaacgcca tcggcaacct cacgaacgcg cgtgcttgcc
     1561 tcactccgtt ggtcgtcacg gcgggacagc aggactaccg gcacatcgtc accgaccccc
     1621 tgctggccgg cgacctcacc ggcatcgcgc gagcggtctc gaagtggacg cacgaagtgc
     1681 gcacgaccga cgagctcggg acggtgttgc ggcgtgcctt tcacgacgcg atgagccctc
     1741 cggccggacc cgtcttcgtg tcgatcccca tggacctgct cgacgcgtcg accgacgctc
     1801 cggtgccgtc gccgtcggcg atccaccgac gatccgtcgc cgacggtctg gacgagctgg
     1861 cgtcgttact caccgagcgg cccatcggca c
//
LOCUS       DATBFZ010000172         8728 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_4051482, whole genome shotgun sequence.
ACCESSION   DATBFZ010000172 DATBFZ010000000
VERSION     DATBFZ010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 8728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8728
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4051482"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            150..1196
                     /locus_tag="VH419_05095"
     CDS             150..1196
                     /locus_tag="VH419_05095"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEY7043026.1"
                     /translation="MRDNVFEELDTRLQTWSRDQDLSAGVLLTQAGTTIFEGCYGLAD
                     RSSGAPITPRTRFGLASVTKMFTAVAVADLVAADRLRFSDAVADVLPVSRRPSTLHSE
                     VTIHHLLTHTSGIADYAEEDEALPWYVEDYGDLWTERPSYSMLRPADFLPLFGDRPPN
                     HKPGEEWHYSNAGYILLGLVIEEVSGRPYVDVVQERVFDRAEMSASGFFRLDEARPDV
                     AVGYLPRGSGDDVWRSNIYRTPVIGGADGGAFSTPRDIDRFLTRLDDGTLVGDVRAQM
                     LARHADAEDDLGYGYGLIHYPDGRIGHGGGDPGVEALVHRIPAEDVNQVVLCNCEGFA
                     GQIRDVVLEAWRRS"
     gene            complement(1369..3588)
                     /locus_tag="VH419_05100"
     CDS             complement(1369..3588)
                     /locus_tag="VH419_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008357822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEY7043027.1"
                     /translation="MTETLTAAGVVPVEESTIRYAIDSDGIVLLTLDDPRSAANTMND
                     QYAASMAGVVDRLYTERDEITGVVITSAKKTFFAGGDLVSLAKTTPADAEAVFDQLQR
                     IKADLRRLELLGKPVVAAINGSALGAGLEIALATHHRIALDDGRLEIGCPEVTLGLLP
                     GAGGVTRTVRMLGLQDALLNVLLKGQRHRPAAALALGLVDELVADREALLPAARKWVL
                     DHRDDAGVTTQPWDRPEYRMPGGIPSSPTLAQHLPAFPANLRKQLKGAPYPAPRAILA
                     AAVEGAQTDFETATRIESRYFVDLVTGPISKNMIQAFFFDLQAINSGTSRPNDVPAYT
                     ARKVGIIGAGMMGAGIAYECARAGMEVVLEDVSVDAAGKGKAHSLRLLDKQVDQGRLS
                     TEQREEILARIQPAGDPSELAGCDLVIEAVFEDQELKHRVFREVEEVVDGDALLCSNT
                     STLPITGLAEAVQRQRDFIGLHFFSPVERMPLVEIIVGEQTSDEAVAKAYDVARQIKK
                     TPIVVNDSRGFFTSRVFGTLVLEGVAMLGEGIDPITIERAATQMGFPAPPLAMVDEVS
                     LVLPRKIAAEAAKAAEASGAGAFASPAMAVVDRMVDEFGRLGKAAGAGFYEYPADGRK
                     FLWPGLWEHGVDPAKNPVDDATFRDLQERFTFVMSLETIRCVEEGVLRSTAEANIGSI
                     FGIGFPALYGGALQYVNGYAGGVAGFAARAQELAASYGPRFTPPPLLLTHAETNSPF"
     gene            complement(3636..4868)
                     /locus_tag="VH419_05105"
     CDS             complement(3636..4868)
                     /locus_tag="VH419_05105"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020386257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEY7043028.1"
                     /translation="MTATTSGIPDAFVYDAIRTPRGRGKKTGALHEVKPVNLVVGLID
                     ELRRRLPEIDPNRIDDVVLGCVTPIGDQGADIAKVSAIAAGLPDTTGGVQLNRFCSSG
                     LEAVNQAAQRIRSGWEDLILAGGVESMSRVPMASDGGAWAMDPETAFATDFVPQGIGA
                     DLIATLGGWSRTDVDAYAAESQSRAAKAEANGHFIRSVIPVKDRNGLTILDHEEFIRP
                     GTTVESLAGLTPSFATIGEQGGFDDVALEKYHWVESIDHVHHAGNSSGIVDGASLVAV
                     GSGAIAEYYGVSPRARIVSTAVSGSDPLIMLTGPAPAARKALAKAGMEVDDIDLFEMN
                     EAFAAVVLRFMCDLDLPHEKVNVNGGAIAMGHPLGATGAMLLGTVIDELERRDQRYGL
                     VTLCVGGGMGVATVVERL"
     gene            4993..5580
                     /locus_tag="VH419_05110"
     CDS             4993..5580
                     /locus_tag="VH419_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043029.1"
                     /translation="MKKLLVVAVSALVLSLSGCGGADDVSNADEGSGSPTSPSTESPT
                     TSETEPTEATEPSDSTEPTSGTLKGGPYCDALQHAKEHLSDLDISQLDEDAFTAMNQE
                     LAKISAAAPADVKDDWATFQQALTRLHQLLASANISFDDLQQLQQGQLPPGVDPQELQ
                     RIAPKIQKISQAPRFQKAGRAISASAKRECHVSLD"
     gene            complement(5634..6773)
                     /locus_tag="VH419_05115"
     CDS             complement(5634..6773)
                     /locus_tag="VH419_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017934997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEY7043030.1"
                     /translation="MAHGPLAGVRVVELAGIGPAPFAGMLLAELGADVVKVDRPSGTA
                     LGVPAEHDLLNRGRPSVALDLKHPEGVETVRRMVDLADVFLEGFRPGVAERLGLGPDQ
                     LLACRPALVYGRMTGWGHTGPLAETAGHDITYLATNGTLHAIGTPADPVVPLNVVGDF
                     GGGAMYLVVGVLAALHSVATTGRGQVVDATIVDGSAHLMTLVHGLLAAHAWSDARGSN
                     LLDGGAPFYGVFATSDGRHVAVGPLERQFYAEFEALLGQDDPLPDRDDVELWPELRSR
                     LAAVFAARPLSHWQAVFEGSDACVAPVLSLREAHTHPHLAARGTFETRAGVVQPAPAP
                     RFSGTPTRLTTPPVPTGHDTRQALLDWGITDADDLIAAGVAVQTA"
     gene            complement(6848..7318)
                     /locus_tag="VH419_05120"
     CDS             complement(6848..7318)
                     /locus_tag="VH419_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEY7043031.1"
                     /translation="MLDPADQRFLMVALEEARTSRREGGIPVGAALVIDGEVRGVGHN
                     QRIQLGSVIRHGETDCLENIGRLTAGVYRRSCLYTTLSPCDMCTGAILLYGIGRVVIG
                     ENTTFTGPEHLLHEAGVEVVVADDEQCVQMMRAFIEEHPALWFEDIGADNPDTA"
     gene            complement(7326..8411)
                     /locus_tag="VH419_05125"
     CDS             complement(7326..8411)
                     /locus_tag="VH419_05125"
                     /EC_number="1.1.1.306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013861204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050607 - mycothiol-dependent formaldehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0010127 - mycothiol-dependent
                     detoxification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)mycothiol dehydrogenase"
                     /protein_id="HEY7043032.1"
                     /translation="MPQVVQGVIARKKGAPVELVDVTVPDPGPGEAVVKVSACGVCHT
                     DLHYREGGIGDDYPYLLGHEAAGVIESVGAGVIDVRPGDFVILNWRAVCGQCRACRRG
                     RPWYCFNTFNADQKMTLADGTELSPALGIGAFLAKTLVHSGQCTKVDEAAPAAVAGLL
                     GCGVMAGLGAAINTGGVTRGDTVAVIGCGGVGAAAIAGARLAGARTIVGIDIDARKLG
                     KAAELGATHTLNGRDEDVVERVKELTDGFGADVVIDAVGRPETYEQAFYARDLAGTVV
                     LVGVPTPEMKLELPLLDVFGRGGALKSSWYGDCLPSRDFPMLIDLYLQGRLPLDAFVS
                     ETIGIGDVEAAFDKMHTGDVLRSVVEL"
BASE COUNT         1397 a         3041 c         2875 g         1415 t
ORIGIN      
        1 catgagaccg gtctactccc cctcccgccg aggtgccagg ttctgccccg gacacgccgg
       61 ttcgcgggct gtcgtcgaca cctcgacccc ggctgtcgat gcaaagaatc gagttgccat
      121 cgatgctgga ccgctgtcac cgtgagtcgg tgcgagacaa tgtcttcgag gagctggaca
      181 cgcggctgca gacatggtcg cgtgaccagg acctgtcggc cggcgtactc ctgacccagg
      241 caggcaccac catcttcgag ggctgctacg ggttggccga ccggtcgagc ggggcgccga
      301 tcacacctcg gactcgcttc ggcttggcct cggtgacgaa gatgttcacg gcggtcgccg
      361 tcgccgacct cgtcgctgca gatcgtctcc gcttctcaga cgctgtcgcc gacgtgctac
      421 cggtgtctcg tcggccttcg acgttgcact cggaggtcac gatccaccac ctgctcacgc
      481 acacctcggg catcgccgac tacgccgagg aggatgaggc gctcccgtgg tacgtcgagg
      541 actacggcga tctctggacg gagcggccct cctactcgat gcttcgccca gcggacttcc
      601 tgccgctctt cggcgatcga ccgcccaacc acaagcccgg cgaggaatgg cactactcga
      661 acgccggcta catcctgctc ggactcgtca tcgaggaagt gtcaggccgg ccgtacgtcg
      721 acgtcgtaca ggagcgcgtc ttcgacagag ccgagatgtc agccagcggg ttcttccggc
      781 tggacgaggc tcgtccggac gtcgcggtcg gctacctgcc gcgtggctcc ggtgacgacg
      841 tgtggcggtc gaacatctac cggaccccgg tcatcggtgg cgccgacggc ggcgctttca
      901 gtactccgcg cgacatcgac cgcttcctga cccggcttga cgacggcacc ttggtcggcg
      961 acgtcagggc ccagatgttg gcgcggcacg cggacgcaga ggacgacctc ggctacggct
     1021 atggcctgat ccactacccg gacggccgca tcgggcacgg tggtggagat cccggcgtcg
     1081 aggcgctggt gcaccggatc ccggccgaag acgtcaacca ggtcgtgctg tgcaactgcg
     1141 aaggcttcgc cggccagatc cgcgacgtcg tactcgaggc ctggcgacgc tcctgaacag
     1201 ccaacgccca cggactgtcg cgggcagacc caacgtttgc tgaccggcac cgcccagggg
     1261 cgactcggca tcgcagaccc cctgtcccca tcgagggttt gcgatgttta cacgccgctt
     1321 gggctgttta catcgcaaac ccatacctcc agtgaaggag tgaaagggtc agaacgggga
     1381 gttggtctcg gcgtgggtga ggaggagtgg cggcggtgtg aagcgaggac cgtacgacgc
     1441 ggccagctcc tgcgcgcgag cggcgaaacc ggccacccca ccggcgtacc cgttgacgta
     1501 ctgcagcgca ccgccgtaca gcgcaggaaa cccgatcccg aagatcgagc cgatgttcgc
     1561 ctcagccgta gaacgcagca cgccctcctc gacgcaccgg atcgtctcca gcgacatcac
     1621 gaacgtgaac cgctcctgca ggtcccggaa cgtcgcatcg tcgaccgggt tcttcgccgg
     1681 atccacgccg tgctcccaca gtccgggcca caggaacttc cggccgtctg ccggatactc
     1741 gtagaagccg gcgccggcgg ccttgcccaa ccggccgaac tcgtcgacca tccggtccac
     1801 cactgccatg gcaggacttg cgaacgcgcc ggcgcctgat gcctccgcag ccttcgcggc
     1861 ctcggcggcg atcttgcggg gcagcaccag cgagacctcg tcgaccatcg cgagcggcgg
     1921 cgccgggaat cccatctgcg tcgcggcccg ttcgatggtg attggatcga tcccctcgcc
     1981 gagcatcgcc acgccctcga gcacgagggt gccgaagacg cgcgatgtga agaatcctct
     2041 gctgtcgttg acgacgatcg gcgtcttctt gatctggcgt gcgacgtcgt acgccttggc
     2101 caccgcctcg tcgctcgtct gctcgcccac gatgatctcg accaggggca tccgctcgac
     2161 cggtgagaag aagtgcagcc cgatgaagtc ccgttgccgc tgcaccgcct ccgccagacc
     2221 ggtgatcggc agcgtcgacg tgttcgagca cagcagcgcg tcgccgtcga cgacctcctc
     2281 gacctccctg aagacccggt gcttgagctc ctggtcctcg aacaccgctt cgatgaccag
     2341 gtcacacccc gccagctccg agggatcgcc agccggctgg atccgggcaa ggatctcttc
     2401 ccgctgctcc gtcgacagcc ggccctggtc gacctgcttg tcaaggagcc tgagggaatg
     2461 tgccttgccc ttcccggcgg cgtcgacaga gacgtcctcg aggacgacct ccatcccggc
     2521 tcgtgcgcac tcatacgcga tgccggcccc catcatcccg gcgccgatga tgccgacctt
     2581 gcgcgccgtg tacgccggca cgtcgttcgg acgagaggtt ccggagttga tcgcctgcag
     2641 gtcgaagaag aacgcctgga tcatgttctt gctgatcggg ccggtgacga gatcgacgaa
     2701 gtatcgcgac tcgatccggg tggcggtctc gaagtcggtc tgggcacctt cgaccgcggc
     2761 cgccaggatg gcccgcggcg cagggtaggg cgctcccttc agctgctttc gaaggttggc
     2821 ggggaacgcg gggaggtgct gcgcgagcgt tggtgacgag gggatcccgc ctggcatccg
     2881 gtactccggc cggtcccacg gctgggtcgt caccccggcg tcgtcacggt ggtcgaggac
     2941 ccacttccgc gcggccggca gcagggcttc gcgatccgcg acgagttcgt ccacgagacc
     3001 gagcgccaac gcagccgcgg gccggtggcg ctgtcccttc agcagcacgt tcagcagcgc
     3061 gtcctgcagg ccgagcatgc gcacggtgcg ggtgacgccg ccagctccgg ggagcagtcc
     3121 cagtgtgacc tcgggacagc cgatctccag tcgaccgtcg tcgagtgcga tccggtgatg
     3181 cgtcgcgagg gcgatctcca gcccggcccc aagggctgag ccgttgatgg ctgcgacgac
     3241 aggcttgccc agcagctcca gccgccgcag gtcggccttg atccgttgca gctggtcgaa
     3301 gaccgcctcg gcatccgctg gagtcgtctt ggccaaggag acgaggtcgc caccggcgaa
     3361 gaacgtcttc ttcgccgacg tgatcaccac acccgtgatc tcgtcgcgtt cggtgtacaa
     3421 ccggtcgacc acgcctgcca tggaggcggc gtactggtcg ttcatcgtgt tggcggccga
     3481 gcgcgggtcg tcgagggtga gcaacacgat gccgtcactg tcgatcgcat accggatcgt
     3541 gctctcctcc accggcacga cgcccgcagc agtcagggtc tcggtcatgt cggtgctcgc
     3601 ttcgtcagtc gggtctgggg gctggtgggt aggcgctaga ggcgctcgac gacggtggcg
     3661 acgcccatgc cgccgccgac gcacagcgtc acgagtccgt accgctggtc acgccgctcg
     3721 agctcgtcga tgacggtgcc gagcaacatc gcaccggtcg cgccgagcgg gtggcccatc
     3781 gcgatcgcac cgccgttgac gttgaccttc tcgtgcggga ggtcgaggtc gcacatgaag
     3841 cggagtacga cggcggcgaa ggcttcgttc atctcgaaca gatcgatgtc atcgacctcc
     3901 atacccgcct tcgccagtgc cttccgggcc gccggagcag gaccggtcag catgatcaac
     3961 ggatcggaac cgctgacggc ggtcgacacg atccgagccc gcggactgac gccgtagtac
     4021 tcggcgatcg cgccgctgcc gaccgcgacc agcgacgcac cgtcgacgat gccggaggag
     4081 ttcccggcgt ggtggacgtg gtcgatgctc tcgacccagt ggtacttctc gagcgccacg
     4141 tcgtcgaagc cgccttgctc accgatggtg gcgaagcttg gtgtgagccc ggccaggctc
     4201 tcgaccgtgg tgcctggccg gatgaactcc tcatggtcga ggatcgtgag cccgttgcgg
     4261 tccttgaccg ggatcacgga ccggatgaag tgcccgttgg cttcggcctt ggcggccctg
     4321 gactgggact cggcggcgta cgcgtcgacg tcggttcgtg accagccgcc gagggtcgcg
     4381 atcaggtcgg ccccgatgcc ctgcggcacg aagtcggtgg cgaaagcggt ctccgggtcc
     4441 atcgcccagg cgccgccatc ggaggccatc ggaaccctgc tcatcgactc cacgccgccg
     4501 gcgaggatca ggtcctccca tccggacctg atgcgttggg cggcctggtt gacggcctcg
     4561 agaccgctgg agcagaacct gttgagctgg acgccgccgg tcgtgtcggg aagccccgct
     4621 gcgatcgccg agaccttggc gatgtcggcg ccctggtcgc cgatcggcgt cacgcagccg
     4681 aggacgacgt cgtcgatacg gttcgggtcg atctcgggga gtcgccgccg cagctcgtcg
     4741 atgaggccca cgaccaggtt gacgggcttc acctcgtgga gcgccccggt cttcttgccg
     4801 cgtccgcgtg gtgtgcggat cgcgtcgtac acgaaggcgt ccgggatacc gctcgtggtg
     4861 gccgtcatgg gtgctccgcc tcgacgtcag ggctaagtta ctcacgagta tagaacggca
     4921 gtcggtacgc cggaagcgtt cggcgagtgg gttgtttcac actgccccac cacgcgtagg
     4981 tacgcttcgg cgatgaagaa gctgctggtc gttgccgtgt ccgcgctcgt gctctcgttg
     5041 agcggatgcg gtggtgccga cgacgtctcg aacgccgacg agggttccgg ttcaccgacc
     5101 agcccgagca ccgagtcgcc gaccacgtcg gaaacggagc cgaccgaggc gaccgagcca
     5161 tccgactcga ccgagccgac cagcggtacg ttgaaaggcg gcccgtactg cgatgcgctc
     5221 cagcacgcca aggagcacct gtcggacctc gacatctcgc agctcgacga ggatgccttc
     5281 accgcgatga accaggaact ggccaagatc tctgccgctg cacccgccga cgtgaaggac
     5341 gactgggcca cgttccagca ggcattgacg cggctgcacc agctgctggc gtccgcgaac
     5401 atctcgttcg acgacctgca gcagctgcag cagggacaac taccgccggg ggttgatccg
     5461 caggagctgc agcgcatcgc cccgaagatc cagaagatca gccaggcacc gcgcttccag
     5521 aaggcgggcc gcgcgatcag cgccagcgcg aagcgcgagt gccacgtctc cctcgactga
     5581 cgaccccgac cgaccctcaa atcccggttt ccgtcggatc aaccgcatag gcgtcaggcg
     5641 gtctggacgg cgacgccggc ggcaatcagg tcgtcggcgt cggtgattcc ccagtcgagg
     5701 agcgcctgtc gggtgtcgtg accggtcggg accggcgggg tggtgaggcg cgtcggcgta
     5761 cccgagaacc ggggcgccgg tgccggttgg acgacacctg cccgcgtctc gaacgtgccc
     5821 cgcgcggcaa ggtgcgggtg cgtgtgcgcc tcgcgcaacg agaggacggg cgccacgcac
     5881 gcgtcggatc cctcgaagac tgcctgccag tgcgacagcg gccgggcagc gaagaccgca
     5941 gccagccgcg atcgaagctc cggccacagc tcgacgtcgt cgcgatccgg caatggatcg
     6001 tcttgaccga gcagcgcctc gaactcggcg tagaactgcc gctcgagtgg acccaccgcg
     6061 acatggcgac cgtcgctggt cgcgaacacg ccgtagaacg gagcgccacc gtcgaggagg
     6121 ttcgacccac gagcatccga ccaggcgtgc gcagccagga ggccgtggac caacgtcatc
     6181 aggtgcgccg acccgtcgac gatggtggcg tccacgacct ggccacgccc cgtcgtggcg
     6241 acggagtgca gggccgcgag cacgccgacc accaggtaca tcgcgccacc gccgaagtca
     6301 ccgacgacat tgagcggcac gacggggtcg gccggtgtgc cgatcgcgtg cagtgtcccg
     6361 ttggtcgcga ggtaggtgat gtcgtggccg gccgtctcgg ccaggggccc ggtgtgtccc
     6421 cagcccgtca tccgtccgta gacaagggca ggccggcacg ccagcagttg gtcaggtccg
     6481 agtccgagtc gttccgccac gccgggtcgg aatccctcca ggaacacgtc ggccaggtcg
     6541 accatccggc gtacggtctc gacaccttcg gggtgcttga gatccaacgc cacgctgggc
     6601 cgaccccggt tcaagagatc atgctcggcg ggcacgccca atgcggttcc cgagggtctg
     6661 tcgaccttga cgacgtctgc gcccagctcg gccagcagca tccccgcgaa tggcgcaggc
     6721 ccgattccgg cgagctccac gacgcgtacg ccggcgagcg gcccatgtgc catcccgcga
     6781 tgataggccg tgtcgaggtg gtgtctacgc cgtgtctacc cgggtctacc cggggtctac
     6841 cccgggtcta cgccgtgtcc gggttgtcgg cgccgatgtc ctcgaaccag agcgccgggt
     6901 gctcctcgat gaatgcccgc atcatctgca cacactgctc gtcgtccgcg accacgacct
     6961 ccacacccgc ctcgtgaagg aggtgctcgg gaccggtgaa cgtcgtgttc tcgccgatca
     7021 cgacacggcc gatcccgtag agcaggatcg ctccggtgca catgtcgcac ggggacaacg
     7081 tcgtgtacag gcaggaccgg cggtagacgc cggcggtgag acgtccgatg ttctccagac
     7141 agtcggtctc gccgtgccgg atcacgctgc cgagctggat ccgctggttg tggccgactc
     7201 cgcgaacctc cccgtcgatg accagcgcgg caccgaccgg aatgccgcct tcacgcctgc
     7261 tcgtacgggc ctcttcgagc gccaccatca ggaaccgctg gtcggccgga tcgagcatgt
     7321 ggcgctcaga gctcgacgac tgaccgcagt acgtcgccgg tgtgcatctt gtcgaacgcc
     7381 gcctcgacgt ccccgatccc gatcgtctcg ctgacgaacg cgtcgagcgg caaccttccc
     7441 tgcaggtaga gatcgatcag catcgggaag tcacggctgg gcaggcagtc gccgtaccac
     7501 gacgacttca aggctccgcc gcgcccgaac acgtcgagga gcggcagctc cagcttcatc
     7561 tccggagtcg gtacgccgac gagcacgacc gtgcccgcca ggtcgcgcgc gtagaacgcc
     7621 tgctcgtacg tctccggtcg gcccaccgca tcgatgacga cgtcggcccc gaagccgtcc
     7681 gtgagttcct tgacgcgttc gacgacgtcc tcgtcccggc cgttgagggt gtgcgtcgcg
     7741 ccgagctccg cggccttgcc gagcttgcgg gcgtcgatgt cgatgccgac gatcgtccga
     7801 gccccggcga gccgagcgcc tgcgatcgcc gcggctccga ctccgccaca accgatcacg
     7861 gcgacggtgt caccgcgcgt caccccgccg gtgttgatcg cggcccccag gccggccatc
     7921 acgccgcagc cgagcagtcc ggcaacggcc gccggcgccg cctcgtccac cttggtgcac
     7981 tggccggagt ggacgagggt cttggcgagg aacgcgccga tgcccagcgc cggtgagagc
     8041 tcggttccgt cggccagtgt catcttctga tcagcgttga acgtgttgaa gcagtaccac
     8101 gggcggcctc gcctgcaggc acggcactgt ccgcacacgg cccgccagtt gaggatcacg
     8161 aagtcaccgg gccgcacgtc gatgacgccg gccccgaccg actcgatcac gccggccgcc
     8221 tcgtgcccga gcaggtacgg gtagtcgtcg ccgataccgc cctcgcggta gtgcaggtcg
     8281 gtgtgacaca caccgcaggc cgacaccttc accacggcct cgcctggccc cgggtccggc
     8341 accgtcacat cgacgagctc gaccggggca cccttcttcc tggcgatgac tccctggacc
     8401 acctgcggca tacgaactcc ttcatcccgc tgatcgagct tgccgagcac ccgctgatcg
     8461 agcttgtcga gcgcccgctg atcgagcttg tcgagatcac cgggctggtc ggcggcaatc
     8521 ggccagcagc gatcggccag cagcgatcgg ggagcgtcca acggacgccc cccgaactgg
     8581 atcccttgat ctcgacgggc tcgatcagcg ggatccgccg ctgctactcg acgtcctcgt
     8641 ccacccagtc aaaggtcttc gtgacggcct tcttccagtt ccggtacgtc cgctcccgaa
     8701 caccttcgtc catctgcggt gaccagcg
//
LOCUS       DATBFZ010000173         7305 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_444738, whole genome shotgun sequence.
ACCESSION   DATBFZ010000173 DATBFZ010000000
VERSION     DATBFZ010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 7305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7305
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444738"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            215..880
                     /locus_tag="VH419_05130"
     CDS             215..880
                     /locus_tag="VH419_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012920894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY7043033.1"
                     /translation="MPDACDDAPTRERVATAILENGPSTAAALAERLDLTPAAVRRHL
                     DHLVGEGAVESREPRVYGARGRGRPAKVFVLTDSGRDTFVQSYDDLAASALRFLRETG
                     GEAAVADFARRRTSELEERYRPVVESAAPAERAKVLAEALSGDGYAASVQSAPVGDQL
                     CQHHCPVAHVAAEFPQLCEAETEVFARLLGRHVQRLATIAHGDGICTTNIPDTTRLGR
                     TSS"
     gene            877..2295
                     /gene="sufB"
                     /locus_tag="VH419_05135"
     CDS             877..2295
                     /gene="sufB"
                     /locus_tag="VH419_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019633769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEY7043034.1"
                     /translation="MTSIEQLNPELEGIGRYEYGWADPDVAGASAQRGLNEAVVRDIS
                     GRKNEPEWMLERRLKGLKLFERKPLPAWGADLGTIDFDNIKYFVRSTEKQASSWEELP
                     EDIKNTYDRLGIPEAEKQRLVAGVAAQYESEVVYHKIRDDLEEQGVIFLDTDTGLREH
                     EDIFKEYFGSVIPVGDNKFASLNTAVWSGGSFIYVPKGVHVEIPLQAYFRINTENMGQ
                     FERTLIIVDEDAYVHYVEGCTAPIYSSDSLHSAVVEIVVKKGARCRYTTIQNWSNNVY
                     NLVTKRATCEAGATMEWVDGNIGSKVTMKYPAVYLLGEHAKGETLSIAFAGEGQHQDA
                     GAKMVHAAPHTSSSIISKSVARGGGRTSYRGLVQVADGAHHSASTVRCDALLVDQISR
                     SDTYPYVDVREDDVSMGHEATVSKVSEDQLFYLMSRGLAEDEAMAMIVRGFIEPIARE
                     LPMEYALELNRLIELQMEGAVG"
     gene            2493..3677
                     /gene="sufD"
                     /locus_tag="VH419_05140"
     CDS             2493..3677
                     /gene="sufD"
                     /locus_tag="VH419_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEY7043035.1"
                     /translation="MTDKGTAGLAGPVETVESHLHPAPSYDLADHPMPVGTEEVWRFT
                     PLKRVRGLLADEPSDARLKWDAEVPAGVTIREISSEDAAGLGEIKPADRPSALAVANA
                     GGAVLVDIPAEAELAAPLIVRLSGMSADETVWGHVIVRVGNHAKAQVVFEHSGSSQYS
                     AITSVLVGDGATLDLVSLQLWEDDAVHLGQIGVRVGRDAHVRTFQASVGGDVVRINEN
                     AEYDGPGGELEMYGLYFADAHQHLEHRLFVDHNAPHTKSNVVYKGGLQGKGAHSVWVG
                     DVLIRKAALGIETFETNRNLVLTDGCRADSVPNLEIETGEIQGAGHASATGRFDDEQL
                     FYLQSRGITEDEARRLVVHGFFADIVRKVGIEEVETRLMSAVETELAQNVGMPVASAV
                     GA"
     gene            3674..4045
                     /locus_tag="VH419_05145"
     CDS             3674..4045
                     /locus_tag="VH419_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020384810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HEY7043036.1"
                     /translation="MSNFRPACALADVPVGGVIAVDVEGVDIAIVRTSEADDEVFAIR
                     DECSHAAIALSEGEVDTEGCEIECWLHGSRFDLRTGKPTGLPAYEPVAIYPCRVDHGT
                     PGNEDGMVLVDVDHPINETEL"
     gene            4051..4809
                     /gene="sufC"
                     /locus_tag="VH419_05150"
     CDS             4051..4809
                     /gene="sufC"
                     /locus_tag="VH419_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEY7043037.1"
                     /translation="MATLDIKDLHVAVDTEDGRKPILRGVDLTVKSGETHAIMGPNGS
                     GKSTLAYSIAGHPKYTITGGTVTLDGVDVLGMTVDARARAGLFLAMQYPVEVPGVSVA
                     NFLRTAKTALDGEAPKLRTWIKDVNDALDQVKMDREFAQRNVNEGFSGGEKKRNEIVQ
                     LELLNPRFAILDETDSGLDIDALKVVAEGVNRFRGQGNRGVLLITHYTRILRYIQPDF
                     VHVFVGGRIAEEGGPELAEQLEAEGYDKYVSASV"
     gene            4844..6115
                     /locus_tag="VH419_05155"
     CDS             4844..6115
                     /locus_tag="VH419_05155"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEY7043038.1"
                     /translation="MTTTDHQIVGLLDTDRIRADFPILTRRLDGDRPLVYLDSANTSQ
                     KPRHVIDTMTEHYSRHNANVARAVHQLGAESTAAFEAARDTVAAFIGAPSRDEVIFTK
                     NITEALNLVANTLAWAQGPYAVGPGDEVVITQMEHHSNIVPWQLLTERTGATLRWFGI
                     TDEGRLDLSNIDDLITERTKVVSLVWVSNMLGTVNPVAEIARRAHEVGALVVVDAAQA
                     VPQLPVDVVATGADLVAFTGHKAVGPTGVGVLWGRREVLEALPPFLGGGEMIETVTMA
                     KSTYAAIPHKFEAGTPPIVQAIGLGAALDYLSGVGMAAVAAHERAITAYALERLQGVS
                     GLTILGPTELVDRGGAVSFEIGGIHPHDVSQVLDSLGVAVRAGHHCAKPAHARFGVQS
                     STRASFYLYTTPAEIDTLVDALEQTKRFFRV"
     gene            6134..6583
                     /locus_tag="VH419_05160"
     CDS             6134..6583
                     /locus_tag="VH419_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEY7043039.1"
                     /translation="MDMESLYQEIILDHYKAPHHEGLREPYDAEVHHVNPTCGDEVTL
                     RVRIADGIVEDVSYEAEGCSISQASTSVLTDLLIGQKVDDAMRTHETFVELMQGRGQV
                     EPDEDVLEDGIAFAGVAKFPARIKCALLGWMAWKDATATAVAEEVAR"
     gene            6601..6960
                     /locus_tag="VH419_05165"
     CDS             6601..6960
                     /locus_tag="VH419_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012920901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEY7043040.1"
                     /translation="MNDTTDLPEVDPGGDLAPVSSDDVLEALKDVVDPELGINVVDLG
                     LVYDVALDDSSVATIDMTLTSAACPLTDVIEDQTRMALDGLVNDFKINWVWMPPWGPD
                     KITDDGREMLRALGFNV"
BASE COUNT         1295 a         2419 c         2482 g         1109 t
ORIGIN      
        1 ccgagggggc tcccgcgaat tgcgggaaaa gtcctgcctt cggcctgtgg acaacggcat
       61 ttttgccgca attcgcgggg agccaggcga ccaaggggag gtaaggggag ccttatttga
      121 ggggcgtggc gaattacgac acaatggtgt tgtgaaatcc acgcgcgacc tgacccggca
      181 cgtccgggac gagtccgtgc gtacgccgcg cgagatgccc gacgcgtgtg atgacgcccc
      241 gacccgcgag cgagtcgcca cggcgattct cgagaacggt ccctccaccg ccgcggcgct
      301 ggccgaacgg ctcgacctca cacccgcggc cgtccgtcgg cacctcgacc atctggtggg
      361 cgaaggcgct gtcgagtcgc gtgagcctcg tgtgtacggc gcccgaggac gcggccgacc
      421 cgcgaaggtg ttcgtcctca ccgactccgg gcgtgacact ttcgtccagt cgtacgacga
      481 cctggccgcc agcgcgttgc gcttcctgag ggagaccggt ggcgaggcag cggtggcgga
      541 cttcgcccgt cggcgtacat ccgagctcga ggagcgctat cgaccggtcg tcgagtcagc
      601 agctcccgcc gagcgggcca aggtgctcgc cgaggcgctc agtggggacg gctacgctgc
      661 gtccgtccag agcgcgccgg tcggcgacca gttgtgccag caccactgtc cggtggcgca
      721 cgtggctgcg gagttcccgc agctgtgcga ggccgagacc gaagtcttcg cccggctgct
      781 gggacggcac gtccagcgac tggcgacgat cgcgcacggc gatggcatct gcaccacgaa
      841 catccccgac acgacccgtc ttgggaggac gtcttcatga catcgatcga gcagctcaac
      901 ccggagctcg agggcatcgg ccgctacgag tacggctggg cagaccccga cgtcgcgggc
      961 gccagcgccc aacgcggtct caacgaggcc gtggtgcgcg acatctcggg ccgcaagaac
     1021 gagccggagt ggatgctcga gcgtcggctg aagggcctca agctcttcga gcgcaagccg
     1081 ctacccgcct ggggtgccga cctcggcacg atcgacttcg acaacatcaa gtacttcgtc
     1141 cggtcgacgg agaaacaggc gtcctcgtgg gaggagctgc ccgaagacat caagaacacc
     1201 tacgacaggc tcggcatccc agaggccgag aagcagcgcc tcgtcgccgg tgtcgccgcc
     1261 cagtacgagt ccgaggtcgt ctaccacaag atccgtgacg acctcgaaga gcagggcgtc
     1321 atcttcctcg acaccgacac cggtctgcgc gagcacgaag acatcttcaa ggagtacttc
     1381 ggctcggtga tcccggtcgg cgacaacaag ttcgcctcgc tgaacaccgc ggtctggtcc
     1441 ggcggctcgt tcatctacgt gccgaagggc gtgcacgtcg agatcccgct gcaggcgtac
     1501 ttccggatca acaccgagaa catgggccag ttcgagcgga ccctgatcat cgtcgacgag
     1561 gacgcctacg tgcactacgt cgagggctgc acggcaccga tctacagctc cgactcgctg
     1621 cactccgccg tcgtcgagat cgtggtgaag aagggcgcgc gctgccgcta cacgacgatc
     1681 cagaactggt cgaacaacgt ctacaacctc gtcaccaagc gcgccacgtg cgaggccggc
     1741 gccacgatgg agtgggtcga cggcaacatc ggctccaagg tgacgatgaa gtaccccgcc
     1801 gtctacctgt tgggcgagca cgcgaagggc gagacgttgt cgatcgcgtt cgccggggag
     1861 ggccagcacc aggacgccgg ggcgaagatg gtgcacgccg cgccacacac gtcgagctcg
     1921 atcatctcca agtcggtcgc ccgcggtggc ggacggacgt cgtaccgcgg tctggtccag
     1981 gtcgccgacg gcgcccacca cagcgccagc acggtgcggt gtgacgcgct cctcgtcgac
     2041 cagatcagcc gcagtgacac ctacccgtac gtcgatgtcc gcgaggacga cgtctcgatg
     2101 ggccacgagg ccacggtctc gaaggtgagc gaggaccagc tgttctacct gatgagccgc
     2161 ggactggccg aggacgaggc gatggcgatg atcgtgcgcg ggttcatcga gcccatcgct
     2221 cgggagcttc ccatggagta tgcgctcgag ctcaaccggc tgatcgagct gcaaatggag
     2281 ggcgccgttg ggtgacggcg ctgggcgccg tcccaccaac ggctcgccgc cttcgcctcc
     2341 cgcgcgtggc tgggctcgac attcctggtc gaggctgggt gccgagacgt gccgcctgag
     2401 ccgcgggctc cgctgctgac ggcgtaccga agacacgact ccggcgtccg ggtgagacgt
     2461 gttcgagaag tgaagacgaa gaaagaagag atgtgactga caagggaacg gcagggttgg
     2521 cggggccagt ggagacggtg gagagccatc tccacccggc gccgtcatac gacctggcgg
     2581 atcatccgat gccggtcggg accgaggagg tgtggcgctt caccccgctg aagcgagtgc
     2641 gcggtctcct cgccgacgag ccgtccgacg cccgtctcaa gtgggacgcc gaggtcccgg
     2701 cgggtgtcac gatccgcgag atcagcagcg aggatgcggc cgggctcgga gagatcaagc
     2761 cggccgatcg tccgtctgca ctcgcggtcg cgaacgccgg gggagcggtg ctggtcgaca
     2821 tcccggccga ggccgagctt gcggcaccac tcatcgtgcg gctcagcggt atgtcggccg
     2881 acgagacggt gtggggtcac gtgatcgttc gggtgggcaa ccacgccaag gcgcaagtgg
     2941 tcttcgagca ctcgggcagc agtcagtaca gcgccatcac ctcggtactg gtcggtgacg
     3001 gtgccaccct cgacctggtg tccctgcagc tgtgggagga cgacgccgtc cacctcgggc
     3061 agatcggcgt acgggtcggt cgcgacgcgc acgtccggac cttccaggcc agcgtcggcg
     3121 gcgacgtcgt gcgcatcaac gagaacgccg agtacgacgg cccgggcggc gagctcgaga
     3181 tgtacggcct ctacttcgcc gacgcccacc agcacctcga gcaccggctc ttcgtcgacc
     3241 acaacgcccc gcacaccaag agcaacgtcg tctacaaggg cggactccaa ggcaagggtg
     3301 cccactcggt gtgggtcggt gacgtgctca tccgcaaggc cgcgctgggc atcgagacgt
     3361 tcgagaccaa ccgcaacctc gtgctcaccg acggctgccg ggccgactcc gtccccaacc
     3421 tggagatcga gaccggcgag atccaaggtg cagggcacgc gtccgccacc ggccggttcg
     3481 acgacgagca gctcttctac ctgcagtcgc gtggcatcac cgaggacgag gcacgccgcc
     3541 tcgtcgtcca cgggttcttc gccgacatcg tccgcaaggt gggcatcgaa gaggtcgaga
     3601 cccggctgat gtccgcggtc gagaccgagc tcgcccagaa cgtcggcatg ccggttgcct
     3661 cggcggtggg tgcatgagca acttccggcc cgcctgcgcg cttgccgacg tcccggtcgg
     3721 tggcgtgatc gcggtcgacg tcgagggcgt cgacatcgcg atcgtgcgca cctccgaggc
     3781 ggacgacgag gtgttcgcga tccgtgacga gtgctcgcat gcagccatcg cgctgagcga
     3841 gggcgaggtc gacacggagg gctgcgagat cgagtgctgg ctgcacgggt cgaggttcga
     3901 cctgcggacc ggcaaaccga ccgggctgcc cgcctacgaa ccggtcgcca tctacccctg
     3961 tcgcgtcgac cacggcacac cgggcaacga ggacggcatg gtcctcgtcg acgtcgacca
     4021 tcccatcaac gagacggagc tgtaacacac atggccaccc tggacatcaa ggacctgcac
     4081 gtcgccgtcg acaccgagga cgggcgcaag ccgatcctgc gaggcgtcga cctcaccgtg
     4141 aagtcgggcg agacccacgc gatcatggga cccaacggct cgggcaagtc cacgctggcg
     4201 tactcgatcg ccggccaccc gaagtacacg atcaccggcg gcacggtgac actcgacggc
     4261 gtcgacgtgc tcggcatgac ggtcgacgcg cgggcgcgtg ccggtctgtt cctggccatg
     4321 cagtacccgg tcgaggtgcc tggcgtctcc gtcgcgaact tcctgcgtac ggcgaagacc
     4381 gccctcgacg gcgaggcgcc caaactgcgt acctggatca aggacgtcaa cgacgcactc
     4441 gaccaggtca agatggaccg cgagttcgcg cagcgcaacg tcaacgaggg cttctccggc
     4501 ggtgagaaga agcgcaacga gatcgtccag ctcgagctgc tcaaccccag gttcgcgatc
     4561 ctcgacgaga ccgactcggg cctcgacatc gacgcgctca aggtcgtcgc cgagggcgtc
     4621 aaccggttcc gcggccaagg caaccgtggc gtgttgctca tcacccacta cacgcgcatc
     4681 ctgcgctaca tccagccgga cttcgtgcac gtgttcgtcg gcggccggat tgcagaagag
     4741 ggcggacccg agctcgccga gcaactcgag gcagagggtt acgacaagta cgtgagtgca
     4801 agcgtctgac gacagacgtc gtgacgggac ggaaggagga atgatgacca cgacggacca
     4861 ccagatcgtc ggtctgctcg acacggaccg gatccgtgcc gacttcccga tcctcacccg
     4921 acgccttgac ggcgaccggc cgctggtcta cctcgacagc gccaacacgt cgcagaagcc
     4981 tcgacacgtc atcgacacga tgaccgagca ctacagccgg cacaacgcca acgtcgcccg
     5041 tgccgtccac cagctcggcg ccgagtcgac agcggcgttc gaggcggccc gtgacaccgt
     5101 ggccgcgttc atcggcgcgc cgtcacgcga cgaggtgatc ttcaccaaga acatcaccga
     5161 ggcactcaac ctggtggcca acacgttggc ctgggcgcag ggaccgtacg ccgtcggccc
     5221 gggcgacgag gtcgtcatca cccagatgga gcaccactcc aacatcgtcc cgtggcagct
     5281 gctgacggag cgcacgggcg ccactcttcg ctggttcggg atcaccgacg agggcaggct
     5341 cgacctgtcg aacatcgacg acctgatcac cgagcgcacc aaagtcgtca gcctggtctg
     5401 ggtctccaac atgctcggca ccgtcaaccc ggtcgccgag atcgcccgcc gcgcgcacga
     5461 ggtcggcgcg ctcgtcgtcg tcgacgccgc acaggccgtc ccgcagctgc cggtcgacgt
     5521 ggttgccacc ggtgctgacc tggtcgcctt caccggccac aaggccgtcg gcccgaccgg
     5581 tgtcggcgtc ttgtggggac ggcgcgaggt gctcgaggcg ctgccgccgt tcctcggtgg
     5641 cggcgagatg atcgagacag tgacgatggc gaagtcgacg tacgccgcca tcccgcacaa
     5701 gttcgaggcc gggacacccc ccatcgtgca ggcgatcgga ctcggcgctg cgctcgacta
     5761 cttgtccggt gtcggcatgg ccgcggtcgc cgcgcacgaa cgcgcgatca cggcgtacgc
     5821 gctggagcgg ctgcagggcg tgagcggcct gacgatcctc ggtccgaccg agctggtcga
     5881 tcgcgggggc gccgtcagct tcgagatcgg cggcatccac ccgcacgacg tcagccaggt
     5941 cctcgactcg ctcggggtcg ccgtcagggc tggccaccac tgcgcgaaac cggcgcacgc
     6001 ccgcttcggt gtgcagtcgt cgacgcgggc atcgttctac ctgtacacga ctccggcgga
     6061 aatcgacacg cttgtcgatg cgttggagca gacgaagcga ttcttccggg tctgagaggg
     6121 catcaagaga cgtatggaca tggagagcct gtaccaggag atcatcctgg accactacaa
     6181 ggcaccccac cacgagggtc tgcgcgagcc gtacgacgcc gaggtgcacc acgtcaaccc
     6241 cacctgcggt gacgaggtga ccttgcgggt gcggatcgcg gacgggatcg tcgaggacgt
     6301 ctcgtacgaa gccgaaggat gctccatctc gcaggcgtcg acgtccgtac tgaccgatct
     6361 gctgatcggc cagaaggtgg atgacgcgat gcggacccac gagaccttcg tcgagttgat
     6421 gcagggtcgc ggtcaggtcg agccggacga ggacgtgctc gaggacggca tcgcgtttgc
     6481 aggagtcgcg aagttcccgg cgcgcatcaa gtgtgcgcta ctgggatgga tggcgtggaa
     6541 ggacgcgacc gcgaccgcgg tcgccgagga ggtggcccga tgacgcaggg aatggaacag
     6601 atgaacgaca caaccgacct gcccgaggtc gatccgggcg gcgacctggc gcccgtctcc
     6661 tccgacgacg tgctcgaggc gctcaaggac gtcgtcgacc ccgagctcgg catcaacgtc
     6721 gtcgacctcg gtctcgtgta cgacgtggca ctcgacgaca gctcggtcgc caccatcgac
     6781 atgacgctca cgtcggcagc atgcccgctc accgacgtga tcgaggatca gacccgaatg
     6841 gccctcgacg gtctggtcaa cgacttcaag atcaactggg tctggatgcc gccgtggggg
     6901 cccgacaaga tcaccgacga cggccgcgag atgctgcggg cgttgggctt caacgtctga
     6961 cctgacgccc caactgcgcg gtcgagtgtg tcggggtggc cgctggtgga gcttgtcggg
     7021 gtgaccgctg atcgagcttg tcgagatcaa ggtcccgtcc gctgtggcgt gggttcttgg
     7081 cagcgatgag cgggatgcat ggtgggccgg ctgaggtccg ctgcgggctg tccgatctcc
     7141 ggcggtcgag ggatgccggg ctgaccgctg atcgagcttg tcgagctgac cgctgatcga
     7201 gcttgtcgag atcaaggtcc cgtccgctgt gctcggggtt cttggcagcg atgagcggga
     7261 tgcatggtgg gccggctgag gtccgctgcg ggcactccgg cctcc
//
LOCUS       DATBFZ010000174        15648 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_445928, whole genome shotgun sequence.
ACCESSION   DATBFZ010000174 DATBFZ010000000
VERSION     DATBFZ010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 15648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15648
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_445928"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1504)
                     /locus_tag="VH419_05170"
     CDS             complement(<1..1504)
                     /locus_tag="VH419_05170"
                     /inference="COORDINATES: protein motif:HMM:NF013125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HEY7043041.1"
                     /translation="MVINEIMYHAPDSGTNPNVEFLELYNRGAAAVDVSGWTISDAAV
                     IDTVDHRIPANTVLPSHAFFLIADGLAPGGKGFTDQFPGITPDLRFVAGSNLSNGGEP
                     VTLVDTSDNVVDSVTYDDAAPWPVSPDGTSGSLELVDPFSDNSLGDGWRASAGDYGTP
                     KAQNSVFGTSPGDITNVIATPPRPNPNASFTVQATMPRGLAATLTYKVMFGTDRTTAF
                     TDGANSVGGANDGTYSAVVPGAPAGQLIRFKISADNSGTPLTYPRSDDTRGYDGVVVA
                     DPSLNNASLPVLEWFLPDADYNALLRPVCDDVNYSGVITWQGHVFDNATFRRRGHGSC
                     TDPKPKIEMQLPPGYSVDFNATLPPGAVMAQPFTGPIDEWALQNETYPIPGLGWQNIA
                     AIGDPPNGYLPVRSQHNATFFGAGAILEEYDGAWRQREGYNNSAFYKVEAGGFRTYAT
                     PAQLLASGDFAKKNPDDNDYTDAWQLTQMLNQGPSAAKTAWMLNNIDVAEV"
     gene            2015..2632
                     /locus_tag="VH419_05175"
     CDS             2015..2632
                     /locus_tag="VH419_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020386139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY7043042.1"
                     /translation="MGWYRDRVLPRIQDREMGAARLRTARERVCATLEGDVLEIGFGT
                     GLNTPYYPRTVSKVVAVEPSTVCMRIAEPRVAASTAPVEYGGLTGEHLDLPTGEFDAV
                     LSTWRLCTIPDVDAALSEVRRVLRPGGSFHFIEHGHAPDSKVARWQERLEPINKRVGG
                     GCHLTRRIAEHIERAGFKIEKLDTYYFPGELRPLGYTFEGRAISD"
     gene            2711..3334
                     /locus_tag="VH419_05180"
     CDS             2711..3334
                     /locus_tag="VH419_05180"
                     /inference="COORDINATES: protein motif:HMM:NF037041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043043.1"
                     /translation="MALYHRATITPTKSEVIADWAPTRPWGPSASDPIEVVGAYRFDD
                     PDGRVGMETHLVTAGGALYHVPLTYRDEPLEGAEDALITEMQHSVLGTRWVYDGLRDA
                     RFVIMLAGVAMTGQGEALGMAVYEGRWYIAPTNVRIHGGGWSMERVPVDGFDLVSDDG
                     GPAVLQNDRFELTVPRRPVPGPRPAIGLTATWDGQEKPVVLAEVRER"
     gene            complement(3440..3610)
                     /locus_tag="VH419_05185"
     CDS             complement(3440..3610)
                     /locus_tag="VH419_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043044.1"
                     /translation="MSRDIDKRAVFFLAAALACVLLIPFTPAAHRDVGIVLTCVYLVL
                     AVASWFDFRSNR"
     gene            complement(3607..4602)
                     /locus_tag="VH419_05190"
     CDS             complement(3607..4602)
                     /locus_tag="VH419_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020578156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HEY7043045.1"
                     /translation="MQTVLLYVVIAVALGFDFVNGFHDAANSIATVVATRVLRPQLAV
                     AWAAAFNFIAFLIFGTKVAATIATGVVDPAVLSIGVVFAGLLGAITWNVVTAYLGLPS
                     SSTHALVGGIAGAAVAKGGLSVLVAGGLATIGVFIVYSPLIGVALAFTLMVGVMWLVH
                     RFRSPNRVNRSFRVLQLFSAAAFSLGHGSNDAQKTMGIIFALLIAAGRVTPTSSVPLW
                     VVLTSQAAIALGTLFGGWRIVRTMGTRLTRLKPMGGVCAETAAASMLFFASANGIPVS
                     TTHTITGGIVGVGTAQRMSAVRWNVAQRVVWAWLITIPGTAFVAAATYLLVRLFS"
     gene            complement(4615..5232)
                     /locus_tag="VH419_05195"
     CDS             complement(4615..5232)
                     /locus_tag="VH419_05195"
                     /inference="COORDINATES: protein motif:HMM:NF013982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HEY7043046.1"
                     /translation="MRFRLMPRDEGFYPLFNRAADNAAEGASILRDLVSRPADAAALI
                     DKLREAEHRGDELVKEVHSRLDTAIVTPFDREDILALIEQIDTTVDEMRSAGEFVTLH
                     NIEQPLSGVNELAELLGRAAETAVALIAKLPRLRDLTPSLEEIDRLETEGDAVYRRTM
                     AELFSGEFKAFFVLRWKDILESMEDALNSLEKTGNIVKSIAVKHA"
     gene            complement(5353..6165)
                     /locus_tag="VH419_05200"
     CDS             complement(5353..6165)
                     /locus_tag="VH419_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043047.1"
                     /translation="MGDIACSPTDAHFNGGLGEPGFCQMKATADRASALKPGLVLPLG
                     DEQYQAATASEFAGSYDLTWGRFKAISRPVPGNHEYVSPGAAGYLAYFGQAAEPAGTT
                     WYSFNVAGWHVVALDANCGKIGGCSSTSPEGRWLAADLTANRAVCTLAFWHQPAFSSA
                     QSGGVAASMPLWQAAVAGGVDLILNGHRHLYERFAPADASGRPDSSGAREIVVGTGGE
                     DLEAFGAASPSSEVRLSAFGVLQLTLTPAAYTFRFVDISGHVGDQGGGSCHS"
     gene            6155..6397
                     /locus_tag="VH419_05205"
     CDS             6155..6397
                     /locus_tag="VH419_05205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043048.1"
                     /translation="MSPTATTVAEGGAVVVELGGLAGGVVVGLVGGVSPPPFEAGGGA
                     HATIAHAVSATTAIGPSVRGDIDADRKLAHDPSSRA"
     gene            complement(6528..6938)
                     /locus_tag="VH419_05210"
     CDS             complement(6528..6938)
                     /locus_tag="VH419_05210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043049.1"
                     /translation="MPSDELYEAGATAELIVAAAGYDLVLSYTWAHPADGPQEGVLLA
                     GSPDDEGRVTVAWGDSWHQKPAIIVLTGCLTERVLEVTADYGGGWRWMISLDGRHDDR
                     LLLTMHNVIPEEHATEEEPAGPYPVMVSALQRLS"
     gene            7109..8056
                     /locus_tag="VH419_05215"
     CDS             7109..8056
                     /locus_tag="VH419_05215"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY7043050.1"
                     /translation="MDEPVVEEAAALIAREQAAVRSRRPAMPAAYADPDHCRVALEEL
                     LADGYIGFVAHDEGRAVGVTCGRTSDSVGFIPAHGLAVAPDLVDPTIVVVRLFAEVGP
                     VLLRDGATRFTIDHVDLDPLGGALHNTGFGGGSVFGTQPARPAAGALDVDVRVGTADD
                     LDSIAALSQIEFAQRFAPPIYASPLSRSSAEARELHDRLLHEGAAHFLARRDHTDVGL
                     LTVELTSPAPRLCPNAQPYIGATATHPSQRGQGIGRALVEAALDWAYGRGYETVSVDF
                     ESANPLARPFWLGIGFEPTGYRVRRMIDASYGGHAGTGC"
     gene            complement(8094..8846)
                     /locus_tag="VH419_05220"
     CDS             complement(8094..8846)
                     /locus_tag="VH419_05220"
                     /inference="COORDINATES: protein motif:HMM:NF017932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M90 family metallopeptidase"
                     /protein_id="HEY7043051.1"
                     /translation="MRFWKRRARVLPARWAEVVADSLGEWAYLDADERARLGELIGGL
                     LADKGWEAARGFGLTDEMCTVIAAQAALMVLGQDLDVYRDVRAIIVHPTTVTSTAARP
                     GPVPGVVSEEPIDLLGEAHQQRGPIIVAWDAVLDDVRHPGTGHNVVIHEFAHKLDMLD
                     GLVDGTPPVPGREAFRRWVEVCTAEYQRLRSACGDPLLRDYAAQDPGEFFAVATEAFF
                     DRPEPMRDLKPALYAVFVDYFRQDPAARRQHG"
     gene            complement(8874..9794)
                     /locus_tag="VH419_05225"
     CDS             complement(8874..9794)
                     /locus_tag="VH419_05225"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY7043052.1"
                     /translation="MTLYGVHAGTEGSSIEDVLAYWRKVEALGFEWISVWDHFLPVMG
                     SSRGSFDSVASHAALAITTSRVRVGVLVYSVGYRHPAVLANAIATIDHLSGGRAEASV
                     GAGWAQDEYEAYGLPFPAGRERLDMLAEGVECLAGLLHDERFSFAGRHFQLKEASVGV
                     SPVQPRLPVWVGGMGEKRVIPLAARVADGWDSPLGPSAEEFARKVGVLERECDRIGRD
                     PKSIRRSAHLALVEDEQQLRERFGSDEYSGVGGSVLFGSNDQLLEGVKAFERGGADQI
                     LFAGTVQEGTEQLERVAQLLGLSREPSRSV"
     gene            9917..10711
                     /locus_tag="VH419_05230"
     CDS             9917..10711
                     /locus_tag="VH419_05230"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY7043053.1"
                     /translation="MLEKAGGFLAGDPVRHNLILTLLQARVAHPEPGRYWTVTVDDDP
                     VGVILQSPLDFVATCTPMPAAAVVAGVDAIANIGVRLPGVSGEAATAARFAGHWTERT
                     RSAAWPVVGQRIYEVDQVVPPRDCGGELRQATIEDRDLVVAWFRAFGAEIGENASASD
                     EHALRRLEAGQLWIWQDGEPVAMAAVSATVEGVARVGPVYTEPQRRNRGYASALVASV
                     SAAVRASGAGCILYTDLGNPTSNSIYRALGYQAVSEVLRYRFDQPS"
     gene            complement(10774..10854)
                     /locus_tag="VH419_05235"
     tRNA            complement(10774..10854)
                     /locus_tag="VH419_05235"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10819..10821),aa:Leu,seq:tag)
     gene            complement(10879..13383)
                     /locus_tag="VH419_05240"
     CDS             complement(10879..13383)
                     /locus_tag="VH419_05240"
                     /inference="COORDINATES: protein motif:HMM:NF014044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HEY7043054.1"
                     /translation="MADLHLGELVDPAAHKRNGTPVDLPTSDLTTHGVIVGMTGSGKT
                     GLGIVLIEEALGAGVPCLLIDPKGDLTNLCLTFPHLAAADFRPWINDGDAQKAGVTPD
                     AFAEQQATAWKDGLASWSIGPDRIAALRQAVSFTIHTPGSTSGVPVNIVGSLQAPADT
                     SDMEVVRDEIEGYVSGLLGLVGISADPLSSREHILLSNLIETSWMQGRSLDLGTLVGQ
                     VQQPPVRKLGVFELDQFFPPNDRTALAIKLNGLLASPSFAAWAEGPPLSIDALLHSPD
                     GKPAAAIVTIAHLSDEERQFVVALLLSKVVTWMRRQSGTTDLRTLVYMDEVAGYVPPT
                     ASPPTKQPIMTLMKQARAFGVGLVLSTQNPVDLDYKAISNAGTWMVGRLQTERDKDRL
                     LEGMSAAAGGVDVKTVGDTISGLGKREFVLRRAGKDAPDVFTTRWAMSYLRGPLTRDQ
                     IGTLMADQRSAASAAGAASAVAPTAPVPAPTAAVSAIAPAAPAPSDTSVAAAVSTAPA
                     VAAAPAAPAVPPTSDDASPLMPEVAQGVGVRWVAPAATWLGTVGGAAGSGRYAAAAIA
                     RVHLRYDEEKADLVVNDEYEAVLHPLTAAADPSAAISVDYDDRDLLTAAPATISYVLP
                     DAPLKAKAFWSNLEKDLVAQLVRSRSIEIFANRPLKLFSRTGETQEQFATRCKEAAES
                     GADADVAKLRDKYATKTQSLQSQLNAAQDRAEVAASEAKGRQHEELISTATSILSGFL
                     GGRRRSSRLATDIRRAAGQRSRTSTAGQRVDAAQGKVADLTSRMADLEGELSEEITGI
                     TSAWDEKAAAIEPVSVGLEKTDVQVAQLVLAWVPVA"
     gene            13382..14074
                     /locus_tag="VH419_05245"
     CDS             13382..14074
                     /locus_tag="VH419_05245"
                     /inference="COORDINATES: protein motif:HMM:TIGR02453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HEY7043055.1"
                     /translation="MARTVTSSPMSFRGWPADALDFYRGLEADNSKTYWQANKARYED
                     SVKAPFEALIDELDPEFGPMRMFRPYRDVRFSKDKSPYKTAAAIYGEREGGTSYYLHL
                     STSGLFAGSGSYHMAPDQLVRFREALDDPAKGGEIEAICAGLEKKGHSIGAAEELKSA
                     PRGYAKDHPRISFLRRKGLVAGFEFPIAAWLHTPKAKDRVVKVWREQAPLDDWLDARV
                     GPSTLPPDDAPR"
     gene            14162..>15648
                     /locus_tag="VH419_05250"
     CDS             14162..>15648
                     /locus_tag="VH419_05250"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HEY7043056.1"
                     /translation="MEATRAVRAGSVEELERDGRLLTKVGSLPVLVVSHDGCAFAIED
                     RCPHLGFPLHQGTVEAGLVTCHWHHARFDLASGCTLDPWADDAMGFDVRVEGADVLVT
                     ARPETDPVGRLRRRLNDGLEQGITLVLAKATLGLLEADASDDIIRTALDFGTTYRDQG
                     WGSGLTVLVAMGNLLPLLDPADRALALVQALAFVSRDTRGHAPRFPEPDLGPGHDLER
                     VAGWYRRFVETRSSDAAERSLATAAASGDLAATERMMFDAVTDHVFIDEGHTLDFTNK
                     AFEGLALVGSSDASRVVTSMVRQTCAADRSEEGSEWNHPHDLASLVEHAVAELPAALA
                     DGAAVPDAAVDVTKVAWALLDDDPEAVVGAVLSAVRAGATGEQLGRALAYASALRITR
                     FHLNNDPGDWNSVHHAFTTANGLHQALVRQSGDGPATPELLRGVMHGALRVYLDRFLN
                     VPAARLPQATSGTLDDLHACWDVQGAVDAAGGAAYGYLRGGGSRE"
BASE COUNT         2406 a         5353 c         5349 g         2540 t
ORIGIN      
        1 ccacctcggc cacgtcgatg ttgttcaaca tccaggcggt cttcgccgcg gacggcccct
       61 ggttgagcat ctgcgtgagc tgccacgcgt ccgtgtagtc gttgtcgtct ggattcttct
      121 tggcgaagtc accagaggcc agcagctgtg cgggcgtcgc gtaggttcgg aagccaccgg
      181 cctcgacctt gtagaaggcc gagttgttgt atccctcccg ctggcgccac gcgccgtcgt
      241 actcctcgag aatggcgccg gcgccgaaga acgtggcgtt gtgctggctg cggaccggca
      301 ggtagccgtt cggcgggtcg ccgatcgcgg cgatgttctg ccagccgaga cccgggatcg
      361 ggtaggtctc gttctggagc gcccactcgt cgatcggccc cgtgaagggc tgcgccatca
      421 ccgcgcccgg cggaagtgtg gcgttgaagt cgacggagta gccggggggc agctgcatct
      481 cgatcttggg cttcggatca gtgcacgaac cgtggccgcg gcgccggaag gtggcgttgt
      541 cgaacacgtg gccttgccag gtgatcacgc cgctgtagtt gacgtcgtcg cagaccggcc
      601 gcagcagggc gttgtaatcc gcatccggaa ggaaccactc gaggacgggc agcgacgcgt
      661 tgttcagcga cgggtccgcc actaccacac cgtcgtaccc gcgcgtgtcg tcggatcggg
      721 gataggtcaa cggtgtgccg ctgttgtcag cggagatctt gaaccggatc agctgacctg
      781 ccggcgcgcc cggaacgacg gcgctgtacg tcccgtcgtt ggcaccgccg acgctgttgg
      841 caccgtccgt gaatgccgtt gtccggtccg tgccgaacat caccttgtac gtcagggtgg
      901 cggccagccc cctcggcatc gtggcctgca cggtgaacga cgcgttgggg ttcgggcgag
      961 gcggcgtggc gatgacgttc gtgatgtctc ctggagacgt gccgaacacc gagttctgcg
     1021 ccttcggcgt gccgtagtcg ccggcgctcg cccgccagcc gtccccgagc gagttgtcgg
     1081 agaacgggtc gacgagctcg agcgagccgg acgttccatc cgggctcacg ggccagggag
     1141 cagcgtcgtc gtacgtgacg ctgtcgacga cgttgtccga ggtgtccacc agggtcacgg
     1201 gctcgccgcc gttcgacaag ttgctcccgg ccacgaagcg gaggtcaggc gtgatgccag
     1261 ggaactggtc ggtgaagccc ttgcctccgg gggcgagacc gtcggcgatc aggaagaagg
     1321 cgtgtgacgg taggaccgtg ttggcgggga tccgatggtc gacggtgtct atgacggcag
     1381 cgtcggagat cgtccagcca ctcacatcca ccgcagccgc tccccggttg tagagctcga
     1441 ggaactccac gttggggttc gttccgctgt ccggtgcgtg gtacatgatc tcgttgatca
     1501 ccacgtcgga tgtgtccgcg tgtgctgctg gtactgcaat gagtaccaag ccggttgaac
     1561 tgaaggacgc gacagccagc gccgctgtga gccgcccgag acgccgcccc atttttgcct
     1621 ccaccgttcc cgccaggtcc gctgtcgctc tctcctagtg cgcggcgcga tcggtgtgtc
     1681 ggggacgttg aggcggaatc ggctccagcg gtggcaccgc ctccatgcgg cctcgcgacg
     1741 cggtggcgcg gccacggccg gtgcccgggc ggccggccgg tgtgagcgtg gtacctgcgg
     1801 tgggggcgac ggcgacctcg ccgtacttcg cactcactgt ccacgcagcg cctcggggtc
     1861 gaccacgaag tgctcagcca tggctgttgc ctcctatcgt gcctacgggc ggccggcgtc
     1921 gctcggttcg gcgcggcgaa ctccttgatc ggtgttcgcc gcggcaccca tatggttcgc
     1981 ccggtcggga ggtggctgaa gcagaatctc gggcatgggc tggtaccgcg atcgcgtcct
     2041 accgcggatc caggacaggg agatgggcgc agcacgcttg cgaacagcgc gtgagcgggt
     2101 gtgcgccacc ttggaaggcg acgtgctgga gatcggattc ggaaccggtc tgaacactcc
     2161 gtactacccg cgaaccgtgt cgaaggtcgt cgccgtcgag ccgtcgacgg tgtgcatgcg
     2221 gatcgccgag ccgcgcgtcg cggcctcgac ggcgccagtc gagtacggcg gtctcaccgg
     2281 tgaacacctg gacctgccca ccggcgagtt cgacgcggtg ctctcgacgt ggaggttgtg
     2341 caccatcccg gacgtcgacg ccgccctgtc cgaggtgcgc cgggtgctgc gcccgggcgg
     2401 gtcgttccac ttcatcgagc atgggcacgc gccggattcg aaggtcgctc gctggcagga
     2461 gcgattggag ccgatcaaca agcgtgtcgg gggcggttgc cacctcaccc gccgcatcgc
     2521 tgaacacatc gagcgcgccg gcttcaagat cgagaagctc gacacctatt acttccctgg
     2581 cgagctgcgg ccgctggggt acaccttcga gggacgggcc atcagcgact agcagcagcg
     2641 cctgatctgc cgcgctgcgc tcggccgtcg agcctcctcg gcgacccaac tctcgtacgc
     2701 tgcctactcc atggctctct atcaccgagc gacgatcacg ccgaccaagt ccgaggtcat
     2761 cgccgactgg gcaccgaccc gaccgtgggg tccttccgcc tcggacccga tcgaggtggt
     2821 cggggcgtac cggttcgacg atcccgacgg ccgcgtcggc atggagaccc acctcgtcac
     2881 tgccggaggc gcgctgtacc acgtcccgct cacgtaccgg gacgaaccgc tcgagggcgc
     2941 cgaagacgcg ctcatcaccg agatgcagca ctcggtgctc ggtacccgct gggtgtacga
     3001 cggcctgcgc gacgcgcggt tcgtcatcat gctcgccggg gtggccatga ccggccaggg
     3061 cgaggcgctg gggatggcgg tctacgaggg ccgctggtac atcgcgccga ccaacgtccg
     3121 catccacggc ggcggttgga gcatggagcg agtgccggtc gacggattcg atctcgtctc
     3181 agacgacggg ggacccgcgg tgctgcagaa cgaccgcttc gagctgaccg tgcctcggcg
     3241 tcctgttccg ggaccgcgcc cagcgatcgg actgaccgcg acctgggacg gacaggagaa
     3301 gccggtcgtc ctcgcggagg tgcgggaacg ctaggccgga cctgctcagc ccgtgtgccg
     3361 aggtgcgcac cgcgccgcgg accgtgagct caactgacct gtgaggggcg tgaggtaaac
     3421 ccggctggac ggccacgggc tagcggttgc tgcggaagtc gaaccatgag gcgacggcca
     3481 gcacgaggta cacgcaggtg aggacgatgc cgacgtcgcg atgagccgcg ggtgtgaagg
     3541 ggatgaggag cacgcaggcg agcgcagcag cgaggaagaa gacggcgcgc ttgtcgatgt
     3601 cgcggctcat gagaagagcc tgaccaacaa atacgtggcc gccgcgacga acgcggtgcc
     3661 agggatggtg atcagccacg cccagacgac acgctgggcg acgttccacc tgaccgctga
     3721 catccgctgg gcggtaccga cgccgacgat gccaccggtg atggtgtgcg tcgtggagac
     3781 cgggatgccg ttggcggatg cgaagaacag catgctggcg gccgccgtct cagcgcagac
     3841 gccgcccatc ggcttcaggc gggtcaggcg ggttcccatc gttcgtacga tgcgccagcc
     3901 gccgaacaac gtgccgagcg cgattgcagc ctgtgacgtg aggacgaccc acaagggaac
     3961 gctcgacgtc ggcgtcaccc ggcccgccgc gatcaggagg gcgaagatga tgcccatggt
     4021 cttctgggcg tcgttcgaac cgtgcccgag gctgaacgcg gcggccgaga agagttggag
     4081 tacccggaag ctgcggttca cccggttcgg tgagcggaac cggtggacaa gccacatgac
     4141 gccgaccatg agcgtgaacg ccaatgccac gccgatgagg ggcgagtaga cgatgaagac
     4201 gccgatcgtg gccagtccgc ccgcgacgag caccgacagc ccgcctttcg cgacggccgc
     4261 gccggcgatg ccgcccacga gtgcatgggt tgatgacgac gggaggccga ggtaggccgt
     4321 gacgacgttc caggtgatgg cgcccagcag accggcaaag acgacaccga tcgacagcac
     4381 ggccgggtcg accacgcccg tcgcgatggt cgctgcgacc ttcgtgccga agatcaggaa
     4441 ggcgatgaag ttgaatgccg ccgcccaggc cacagccagt tgagggcgca gcaccctggt
     4501 cgcgaccacg gtggcgatcg agttggcggc gtcatggaag ccgttcacga aatcgaagcc
     4561 gagcgcgacc gcgatgacga cgtagaggag aacggtctgc atcgcgccgg caggtcaggc
     4621 gtgcttgacg gcgatcgatt tgacgatgtt gcccgtcttc tccagggagt tgagggcgtc
     4681 ttccatcgac tcgaggatgt ctttccagcg gagcacgaag aaggccttga actcgccact
     4741 gaacagctcg gccatggtcc ggcggtagac cgcgtcacct tcggtctcga ggcggtcgat
     4801 ctcttcgagc gagggtgtga gatcacgaag ccttggaagc ttggcgatga gcgcgaccgc
     4861 ggtctccgcc gctcggccga ggagctcagc gagctcattg accccgctca gcggctgttc
     4921 gatgttgtgg agcgtcacga actctccggc ggagcgcatc tcgtcgacgg tggtgtcgat
     4981 ctgctcgatc agcgccagga tgtcttcccg gtcgaacggg gtcacaatgg ccgtgtcgag
     5041 ccggctgtgc acctccttga ccagctcgtc gccccgatgc tcggcctcgc gcagcttgtc
     5101 gatgagggcg gcggcgtcgg cgggacggga aacgaggtca cgcagaatgc tggcgccctc
     5161 tgccgcgttg tcggcggcgc ggttgaacaa cgggtagaac ccctcgtccc gcggcatcaa
     5221 cctgaacctc atcgacctcc gtcctcgcgg tgacagcggc agtgtaggtc cggtggtttg
     5281 aggctggacg tgcgccgttt gccctgcgtt catgcatcgt tcaccacttg ctgccgcctg
     5341 gccggtgcgc tttcagctgt ggcagctgcc gcccccttgg tcaccgacgt ggccgctgat
     5401 gtcgacgaat cggaacgtgt acgcggctgg cgtgagcgtc agctggagga ccccgaaggc
     5461 cgacagcctg acctcgctgg acggtgatgc cgctccaaac gcctcgaggt cctcgccacc
     5521 ggtccccacg acgatctccc tcgcccccga tgagtcaggt cgcccggacg cgtcggccgg
     5581 ggcgaaccgc tcgtacaggt gacggtgacc gttgaggatg agatcgacgc cgccagcaac
     5641 tgcggcctgc cagagaggca tcgaggccgc gactccgccc gactgagcgg aggagaacgc
     5701 cggctggtgc cagaacgcca acgtgcacac ggcacggttc gcggtcaggt cagcagcgag
     5761 ccagcgtcct tcgggcgacg tggagctgca gccgccgatc ttcccgcagt tcgcgtcgag
     5821 cgcgaccacg tgccaaccgg cgacattgaa gctgtaccag gtcgtcccgg cgggttcggc
     5881 tgcctggccg aagtaggcga gatacccggc cgctcccggc gacacgtatt cgtggttgcc
     5941 cggcactggg cggctgattg ctttgaatcg cccccacgtc aggtcgtagg agcccgcgaa
     6001 ctccgaggcg gtcgccgcct ggtactgctc gtcgcccaag ggaagaacga gtcccggctt
     6061 cagcgcggat gctcgatcgg cggtcgcctt catctggcag aacccaggtt caccgagccc
     6121 gccgttgaag tgagcgtcgg tcggactgca cgcgatgtcg cccaccgcaa ccaccgtcgc
     6181 cgaaggcgga gccgtggtcg tcgagctggg aggcctcgcg ggcggagtcg tggtcggcct
     6241 cgtgggaggc gtcagcccac cgccgttcga ggcggggggc ggtgcgcacg cgacgatcgc
     6301 gcacgccgtc agcgcgacca cggcaatcgg gccgagtgtc cgtggtgaca tcgacgcaga
     6361 ccgtaagctc gctcacgacc catccagtcg cgcgtgaact gacacgggtc ccgcttcccg
     6421 aggctgtctc ctcggcgccg cgcaccgtca cctttcgccc gccctgagcg cgaccggtaa
     6481 ccgggaccgt gccgccggtg ctgggcaggc ctccgacgcc cccgggatca gctgaggcgc
     6541 tgcaacgccg acaccatgac cgggtacggc cccgccggct cctcctcggt ggcgtgctcc
     6601 tccggaatga cgttgtgcat cgtcaggagc aaccggtcgt cgtgccggcc gtcgagggag
     6661 atcatccacc gccagccccc gccgtagtcg gcagtcacct cgaggacacg ctcggtcaga
     6721 cagccggtga gcacgatgat cgccggcttc tggtgccagc tgtcgcccca cgcaaccgtg
     6781 acacgcccct cgtcgtccgg cgatccagcg agcaagacgc cctcttgagg tccgtcggcg
     6841 ggatgcgccc aggtgtagga caacacgagg tcgtagcctg cggccgccac gatcaactct
     6901 gctgtcgcac ccgcctcgta cagctcgtcg cttggcatca accggaaccc gttggtgcca
     6961 ctccaagccc cgacgtgtcg cccaaagctc ccggccgcgc tcgctctgtc ctgggcagcc
     7021 acgaacgaag gatgccacgc ctccctcatc taccttgacg ccagcgtatc ggaggctgcg
     7081 aagcacgacg atgttgaccc ttcaacgatt ggacgagcct gtcgttgagg aggccgcggc
     7141 cctgatcgct cgtgagcagg cagcggttcg ctcgcgccga ccggcgatgc cggctgccta
     7201 cgccgacccg gaccactgcc gcgtcgcgct cgaggagctc ctggccgacg gctacatcgg
     7261 cttcgtcgct cacgacgagg gtcgtgccgt cggggtgacg tgtggacgga ccagcgattc
     7321 ggtggggttc attccggcgc atggcctcgc ggttgctcct gacctcgtcg accccaccat
     7381 cgtcgtcgtc cgactcttcg ccgaagttgg gccggtcctg ctacgagacg gtgccacccg
     7441 gttcacgatc gaccacgtcg acctcgaccc gctcggcggc gcgctccaca acaccggctt
     7501 cggcggtggg agcgtgttcg ggacccagcc ggcgcggccg gccgctgggg ccctcgatgt
     7561 tgacgttcgg gtcggcaccg ccgacgatct cgattcgatc gccgccttga gccagatcga
     7621 gttcgcccag cgcttcgctc cgccgatcta cgcgtctccg ctgtcgagat catcggcgga
     7681 ggcccgcgag ctccacgacc gtctgctgca cgagggcgcc gcccacttcc tcgcccgtcg
     7741 cgatcacacc gacgtcggct tgctcaccgt cgagctcact tcccccgcgc cgcgactctg
     7801 cccgaacgct cagccctaca tcggtgccac cgccactcac ccctcgcaga gaggccaggg
     7861 gatcggccgg gccctcgtcg aggccgctct ggactgggcc tacggccgcg gatacgagac
     7921 cgtgtcggtc gacttcgaat cggcgaaccc gctggctcga cccttctggc tcgggatcgg
     7981 gttcgagccc accggctacc gcgtccgacg catgatcgac gcctcgtacg gcgggcacgc
     8041 cgggaccggg tgctgagacg cttcgcgctg ggattgcttt gcaggttcgc cgttcaaccg
     8101 tgctgccggc gggcggcggg gtcctggcgg aagtagtcga caaacacggc gtagagcgcg
     8161 ggcttcaggt cgcgcatcgg ctcgggccga tcgaagaacg cctcggtggc gacggcgaag
     8221 aactcgcccg ggtcctgcgc cgcgtagtcg cgtagcagtg ggtcaccgca agccgaacgc
     8281 agccgctgat attcggcggt gcacacctcc acccatcggc ggaacgcctc ccgcccgggc
     8341 accggtggtg tgccgtcgac cagcccgtcg agcatgtcga gcttgtgcgc gaactcgtgg
     8401 atcaccacgt tgtgcccggt ccccgggtgg cgcacatcgt ccaggactgc gtcccacgcc
     8461 acgatgatcg ggcctcgctg ttggtgcgcc tcacccaaca ggtcgatcgg ctcctcgctg
     8521 accacgccgg gcacggggcc tggccgcgca gcggtcgatg tcaccgtcgt gggatgcacg
     8581 atgatcgctc ggacatcccg gtagacgtcc aaatcctgtc cgagcaccat cagagcggcc
     8641 tgggcggcga tgacggtgca catctcgtcg gtgagcccaa agccgcgggc tgcttcccac
     8701 cccttgtcgg cgagcagccc gccgatcagc tcgcccagcc gggcccgctc gtccgcgtcg
     8761 aggtacgccc actcgcccag cgagtcggcc accacctccg cccagcgggc aggcagcaca
     8821 cgcgcacgac gcttccagaa ccgcaccgcg acaggctgcc accgcgcccg tgctcacacc
     8881 gaccgggacg gctcacgtga gaggcccagc agctgcgcga cacgctccag ttgctcggta
     8941 ccttcctgga cggtgcccgc gaagaggatt tggtcagcgc cgcctcgttc gaacgccttg
     9001 acaccttcga gcagttggtc gttcgagccg aacagcaccg aaccgccaac gccgctgtac
     9061 tcgtcgctgc caaaccgttc ccgaagttgc tgttcgtcct cgaccagcgc caggtgagct
     9121 gagcggcgga tcgatttcgg gtctcggccg atgcgatcgc attcgcgttc gagcacaccg
     9181 acctttcgcg cgaactcctc cgccgacggg ccgagtgggg agtcccagcc gtcggccacc
     9241 cgagccgcga gcgggatgac ccgcttctcg cccatgccac ccacccacac cggtagtcgt
     9301 ggttggacgg gggacacgcc gacggatgcc tccttcagct ggaaatgcct gccggcgaag
     9361 ctgaaccgct cgtcatgcag cagtccggcc aggcattcga ctccctcggc cagcatgtcg
     9421 agtcgctcgc ggccggccgg gaacgggagt ccgtaggcct cgtattcgtc ctgcgcccat
     9481 cccgctccga cggacgcctc ggcccggccg ccggagaggt ggtcgatcgt ggcgatggcg
     9541 ttggccagca ctgcggggtg gcgatacccc accgagtaca cgaggacacc gacccgcaca
     9601 cgagatgtcg tgattgcaag agctgcgtgg ctcgccacgg agtcgaacga cccgcggctg
     9661 gatcccatga ccggtaggaa gtggtcccac accgaaatcc actcgaaccc gagcgcctcc
     9721 accttgcgcc aatacgccaa gacgtcctcg atcgaggagc cttcagtccc ggcatgcacg
     9781 ccgtacagcg tcatcggccc accttcctcg gcgagtcctc gagtgttcca aatagcgccc
     9841 ggtcacgccc gggatgcagg ccatcatcgg gtcgatgccg accgcaaggg ttgaggttac
     9901 ggacacgcca gcgcgcgtgt tggagaaggc cgggggcttc ctggctggag atccggtcag
     9961 gcacaacctg atcctgacgc tgttgcaggc gcgggtcgct catccggaac ccggccgcta
    10021 ttggaccgtc actgtcgacg acgacccggt cggcgtgatc ctccagtcgc cgctcgactt
    10081 cgtcgccacg tgcacgccga tgcccgccgc cgcagttgtc gccggggtcg atgcgatagc
    10141 caacattgga gtgcgcttgc cgggcgtgag cggcgaagcg gcgaccgccg ctcgtttcgc
    10201 ggggcactgg accgaacgca cgcgttcggc ggcgtggccc gtcgtcgggc agcggatcta
    10261 cgaagtcgat caggtcgttc cccctcggga ttgcggcggc gagctgcgtc aggcgacgat
    10321 cgaggaccgt gacctggtgg tggcctggtt tcgcgccttt ggcgcagaga tcggggagaa
    10381 cgccagcgcc tcggacgaac atgctctgcg ccgcttggag gccggtcagc tgtggatctg
    10441 gcaggacgga gaaccggtgg cgatggccgc ggtctctgcg accgtagaag gtgtcgcccg
    10501 agtaggtccc gtgtacaccg agccgcagcg gcggaaccgc ggttacgcct cggccctcgt
    10561 ggcctcagtc tccgccgccg tgcgcgcaag tggcgccggc tgcatcctgt acaccgacct
    10621 cggcaacccg acctcgaact cgatctaccg cgccttgggc taccaggccg tcagcgaagt
    10681 cctccgctat cgcttcgacc agccgagctg accgtgcgac gacactgcgg gagcgaccgg
    10741 acgttgccat cccggtgccg cacgcagcac ctgtgcgggc ggtgggagtc gaacccacac
    10801 tccttgcgga accgggacct aaacccggcg cgtctgccat tccgccacgc ccgcgaggtg
    10861 ggggacgacg acagcctgtc aggcgacggg tacccaggcg agcacgagtt gagccacctg
    10921 gacatcggtc ttctcgagcc cgacggacac cggttcgatg gcggcggcct tctcgtccca
    10981 ggccgacgtg atgccagtga tctcctcgct cagctcaccc tccaggtccg ccatccggct
    11041 ggtgaggtcg gcgaccttcc cttgcgcggc gtcgacccgt tggccggcgg tgctcgtccg
    11101 cgaccgctgg ccggccgccc gacgaatgtc tgtggcgagt ctcgagctgc gccgacggcc
    11161 gccgaggaag ccggagagga tgctcgttgc ggtcgagatc agctcctcgt gctgacgtcc
    11221 tttggcttcg gacgcggcca cctcggctcg gtcctgcgcg gcgttcagtt gactttgcaa
    11281 gctttgtgtt ttcgtcgcgt acttgtcgcg cagcttggcc acgtcggcgt cggcaccgct
    11341 ctcggcggct tccttgcaac gagtagcgaa ctgctcctgc gtctcgcccg tccgggagaa
    11401 cagcttgagc ggccgattgg cgaagatctc gatcgacctg gaccgcacga gttgggccac
    11461 gaggtccttc tcgaggttgc tccagaaggc cttcgccttc agcggcgcgt cggggagcac
    11521 ataactgatc gtcgcgggcg ccgcggtcag caggtctcgg tcgtcgtagt cgaccgagat
    11581 cgcggcggac ggatcggctg ccgcagtgag cgggtggagc accgcctcgt actcgtcgtt
    11641 cacgacgagg tcggcctttt cttcgtcata gcggaggtgc acacgggcga tggcggcagc
    11701 ggcgtaccgc ccgctgccgg ctgcgccgcc gacagtgccc agccacgtcg ccgcgggagc
    11761 gacccagcgc acgccgaccc cctgcgcgac ctccggcatg agcgggctgg cgtcgtcgct
    11821 cgtcggtggg acggctggcg cggcgggtgc ggcggcgaca gcgggcgcgg tcgagacagc
    11881 cgcggccacc gaagtgtccg acggggcagg agcggcggga gcgatcgctg acaccgccgc
    11941 cgtcggtgca gggaccggcg cggttggtgc cactgccgac gccgcgccag ccgcgctcgc
    12001 cgcgctccgt tggtcggcca tcagcgtgcc gatctggtca cgggtgagtg gtccgcgcag
    12061 atagctcatc gcccatcggg tcgtgaagac atcgggcgcg tccttgccgg cccgccggag
    12121 gacgaactcc cgcttgccga gtccgctgat cgtgtcgccc accgtcttga cgtcgacccc
    12181 gccggcggcc gcgctcatgc cttcgagcag acggtccttg tcgcgctcgg tctgcagccg
    12241 cccgaccatc catgtgcctg cgttcgagat cgccttgtag tcgagatcga ctgggttctg
    12301 cgtcgatagg accagcccaa ccccaaaggc gcgcgcctgc ttcatcagcg tcatgatcgg
    12361 ttgcttggtg ggcgggctgg ccgtcggcgg cacgtacccg gccacctcgt ccatgtagac
    12421 gagcgtgcgc aggtccgtcg tgccgctctg gcggcgcatc cacgtcacga ccttcgacaa
    12481 cagcagcgcg acgacgaact gccgctcctc gtcggacagg tgggcgatgg tgacgatcgc
    12541 tgccgcgggc ttcccgtccg gtgagtggag cagcgcgtcg atcgacagtg gcgggccctc
    12601 ggcccacgcc gcgaacgacg gtgatgccag gaggccgttg agcttgatgg cgagcgcggt
    12661 gcggtcgttc ggtgggaaga actgatcgag ctcgaagacg ccgagcttgc gcaccggtgg
    12721 ctgttggacc tggccgacga gcgttccgag gtccagcgag cggccctgca tccacgacgt
    12781 ctcgatcagg ttgctcagca ggatgtgctc gcgcgacgac agcgggtcag cggagatccc
    12841 gaccagcccg agcaggccgg acacgtaacc ctcgatctcg tccctcacga cctccatgtc
    12901 gctcgtgtcc gccggcgctt ggagcgagcc gacgatgttg accggcacac ccgacgtcga
    12961 gccgggcgtg tggatggtga acgagacggc ttgccggagc gcggcgatgc ggtcgggtcc
    13021 gatgctccag ctggccagcc cgtccttcca cgccgtcgcc tgctgttcgg cgaacgcatc
    13081 cggtgtgact ccggccttct gcgcatcgcc gtcgttgatc catggccgga agtcggctgc
    13141 cgccaggtgg gggaaggtga gacacaggtt ggtgaggtcg cccttcgggt cgatcagcag
    13201 gcacgggacg ccggcgccga gcgcctcctc gatcagcacg atgccgaggc cggtcttgcc
    13261 cgaaccagtc atgccgacga tcacgccgtg cgtggtgagg tcgctcgtcg gcaggtcgac
    13321 gggtgtgccg ttgcgcttgt gcgcagcagg atcgacgagc tcccccaggt ggaggtcagc
    13381 catggcgcgg actgtaacgt cctcgccgat gagcttccgc ggctggccgg ccgacgcgtt
    13441 ggacttctat cgcggcctcg aagccgacaa ctccaagacc tattggcagg ccaacaaggc
    13501 gcgctacgag gacagcgtga aggccccgtt cgaagccctg atcgacgagc tcgatccgga
    13561 gttcggcccg atgcgcatgt tccgtcccta tcgcgacgtt cgcttcagca aggacaagtc
    13621 gccgtacaag acggccgccg cgatctatgg cgaacgggag ggcggcacga gctactacct
    13681 ccatctgtcg accagcggtc tgttcgcggg gtcgggctcc taccacatgg ctcctgacca
    13741 gctcgtgcgg ttccgcgagg cgttggacga cccggccaaa ggcggggaga tcgaagcgat
    13801 ctgtgcgggc ctcgagaaga agggccactc gatcggggcg gccgaggagt tgaagtccgc
    13861 gcctcgtggc tacgcgaagg accatccccg catctcgttc ctgcgccgga agggtctcgt
    13921 ggccgggttc gagtttccga tcgccgcctg gctgcacacg cccaaggcga aggacagggt
    13981 ggtgaaggtg tggcgcgagc aggcaccgct cgacgactgg ctggatgcgc gggtcgggcc
    14041 gagcaccctg ccacccgacg acgcgccgag gtgagctcat cggcgtcggg cgtccagcgt
    14101 ccgccggtta ttaccgaagc agcgacttga catattgcgc caggcggagc accctggtcc
    14161 catggaagca acgcgcgcgg ttcgggccgg ttcagtcgag gagctcgagc gtgacggacg
    14221 gctgctgacc aaggtcggct cacttcccgt cctcgtcgtc tcccacgacg gctgtgcttt
    14281 cgccatcgaa gatcgatgcc cccacctcgg cttccccctg caccagggca ccgtcgaggc
    14341 cggcctcgtg acgtgccact ggcaccacgc ccggttcgac ctggccagcg gctgcacgct
    14401 cgatccgtgg gcggacgacg ccatggggtt cgacgttcgc gtcgagggcg ccgacgtcct
    14461 cgtcaccgcc cgtccggaga ccgatcccgt cggccgcctg cgccggcggc tgaacgacgg
    14521 cctcgagcaa ggcatcacgc tcgtcctggc caaggcgacg ctcggcctgc tcgaagcgga
    14581 cgccagcgac gacatcatcc gcacggcgct cgatttcggc acgacctacc gcgaccaggg
    14641 ttggggttct ggtctcaccg tcctcgtggc gatgggcaac ctgctgccgc tgctcgaccc
    14701 tgccgaccgc gctctcgcgc tggtccaagc gctggccttc gtcagtcgcg acacccgcgg
    14761 gcacgcgccg cgcttccccg agcccgacct cggcccgggt cacgacctcg agcgggtggc
    14821 cggctggtac cggcggttcg tcgagacgcg ctcgtccgac gcggccgagc ggtccctcgc
    14881 aacggcggct gcgtcgggcg atctcgccgc caccgagcgg atgatgttcg acgcggtcac
    14941 cgaccacgtc ttcatcgacg agggccacac tctcgacttc acgaacaagg cgttcgaggg
    15001 cctcgcgctc gtcggttcga gcgacgccag ccgggtcgtg acgtcgatgg tccgccagac
    15061 gtgcgccgcg gaccgcagcg aagagggaag cgagtggaac cacccgcacg acctcgcctc
    15121 actcgtggag catgccgtcg ctgagcttcc cgctgcactt gctgatggag cggccgtgcc
    15181 ggacgccgcg gtggacgtca ccaaggtcgc gtgggcgttg ctcgacgacg atcccgaggc
    15241 cgtggtcggt gcggtcttga gcgccgtccg agccggcgcc actggcgagc agctcgggcg
    15301 cgccctcgcc tacgcctccg cgctgcggat cactcgtttc catctcaaca acgacccggg
    15361 cgactggaac agcgtccacc acgcgttcac gacggcgaac ggcctccacc aagcgctcgt
    15421 ccggcagtcg ggcgatggac cggccacgcc ggagttgctc cgcggcgtca tgcatggcgc
    15481 gctgcgcgtc tacctcgacc gcttcctcaa cgtgccggcc gcccggcttc cccaggcgac
    15541 gtccggcacg ctcgacgacc tgcacgcgtg ttgggacgtc cagggcgccg tcgacgcggc
    15601 cggcggtgct gcgtacgggt acctccgcgg cggcggctca cgcgagga
//
LOCUS       DATBFZ010000175         1646 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_446388, whole genome shotgun sequence.
ACCESSION   DATBFZ010000175 DATBFZ010000000
VERSION     DATBFZ010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446388"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="VH419_05255"
     CDS             <1..299
                     /locus_tag="VH419_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEY7043057.1"
                     /translation="FLPNPHWVPELRPQTGLDAAVSEYVLQQPAAIEFLERLDALLET
                     VDAGFIRERKQYLNLAVGCTGGKHRSVTMAEELGRRLSARGIETTIVHRDLGRE"
     gene            320..1318
                     /gene="yvcK"
                     /locus_tag="VH419_05260"
     CDS             320..1318
                     /gene="yvcK"
                     /locus_tag="VH419_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007077856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HEY7043058.1"
                     /translation="MPYDDPWREVADEVSPALKVVALGGGHGLAANLRALRSFVTDLT
                     AVVTVADNGGSSGRLRRELGVLPPGDLRQALAALCRDDEWGRTWAQVLQHRFESAGEL
                     HRHAVGNLLIVALWELLGGHVEGLDWVGRLLGAQGRVLPMAAVPIDIAAEVRGAVPDH
                     PEELTTVRGQVEVASTSGLVESVELQPVNPPACPEAVDAIHAADWVIFGPGSWYTSVL
                     PHLLVPELQTALTKAAARRCVTLNLAPQLGETEGFSSSTYLEVLAVHAPDLQIDVVLA
                     DPAGVVDLPMLEETVDGLGAELVIADVAEPGTEGRHDAAKLAEVYASIMGVEVTTR"
     gene            1381..>1646
                     /gene="whiA"
                     /locus_tag="VH419_05265"
     CDS             1381..>1646
                     /gene="whiA"
                     /locus_tag="VH419_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020384835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEY7043059.1"
                     /translation="MAMTAQVKAELANVSVTKSCCRKAEVSSMLRFAGGLHIQGGRIV
                     VEAELDSGASARRIRRDILEIYGHPSDVVVISGGGIRKGTRYVVR"
BASE COUNT          258 a          514 c          614 g          260 t
ORIGIN      
        1 ggttcctgcc caacccgcac tgggtgcccg agctgcggcc gcagaccgga ctcgacgcgg
       61 cggtcagcga gtacgtcctc cagcagccgg cggcgatcga gttcctggaa cggctcgatg
      121 cgctgctcga gaccgtggac gcgggtttca tcagggagcg aaagcagtac ctgaacctcg
      181 ccgtgggctg cacgggcggc aagcaccgca gcgtgacgat ggcagaagag ctcggcaggc
      241 ggctgagcgc ccgcggcatc gagaccacga tcgtgcaccg tgacctgggg cgggaatgaa
      301 cgacgcgacg ccccgggcca tgccgtacga cgacccctgg cgggaggttg ccgacgaggt
      361 ttcaccggcg ttgaaggtgg tggcgctcgg tggcggtcac gggctggcag ccaaccttcg
      421 cgcgctgcgg tcgttcgtca cggatctgac cgccgtggtc accgtggcgg acaacggcgg
      481 gtcctccggg cgccttcgtc gcgagctcgg ggtcctgcca cccggcgacc tgcggcaggc
      541 gttggccgcg ctgtgtcgtg acgacgagtg gggtcggacc tgggcgcagg tgctgcagca
      601 ccgcttcgag agcgccggcg agctgcaccg tcacgcagtc ggcaacctgc tcatcgtggc
      661 cctgtgggag ctgctcggcg gtcacgtcga gggtcttgac tgggtcggtc gtctgctcgg
      721 tgcgcagggg cgtgtgctac cgatggccgc cgtgccgatc gacatcgctg ccgaggtccg
      781 aggcgccgta ccggatcatc cagaggagct cacgacggtg cgtggacagg tggaggtggc
      841 gagcacatcg gggctcgtcg agtcggtcga gctgcagccg gtcaatccgc cggcgtgccc
      901 ggaagcggtc gacgcgatcc atgcggccga ttgggtgatc ttcggaccgg gatcgtggta
      961 caccagtgtc ttgcctcatc tgctggtgcc ggagttgcag acggcgttga ccaaggcggc
     1021 tgcccgtcgc tgtgtgacgc tcaacctcgc gccgcagctg ggggagacgg agggattctc
     1081 gtcgagcacc tatcttgaag tgctggccgt gcacgcgccc gatctgcaga tcgacgtcgt
     1141 gctggccgac cccgcggggg tggtcgacct tccgatgctc gaggagaccg tggacggact
     1201 aggggcagag ctggtgatcg cggatgttgc cgagccggga acagaaggac gacacgacgc
     1261 ggcaaagctc gccgaggtgt acgcatccat catgggagtc gaggtgacga cccgatgagc
     1321 acggcagcgg ttcacgcgca ggaggacaac ccgggcctgc gccgtgggag gatcgggctc
     1381 atggcgatga ccgcccaggt gaaggcagaa ctcgcgaacg tcagcgtcac gaagtcctgc
     1441 tgccgcaagg cggaagtgtc gtcgatgctg cgttttgccg gcggcctgca catccagggt
     1501 ggccgcatcg tcgtcgaagc ggagctcgac tccggggcct cggcccggcg gatccgtcgc
     1561 gacatcctcg agatctacgg ccatcccagc gacgtggtcg tcatcagcgg cggtgggatc
     1621 cgcaaaggga cccggtacgt cgtccg
//
LOCUS       DATBFZ010000176         3789 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_4655009, whole genome shotgun sequence.
ACCESSION   DATBFZ010000176 DATBFZ010000000
VERSION     DATBFZ010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 3789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3789
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4655009"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(156..1184)
                     /locus_tag="VH419_05270"
     CDS             complement(156..1184)
                     /locus_tag="VH419_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015069427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEY7043060.1"
                     /translation="MDEGQREMSESMRAWTFDAPAPVPEVSLTLATRPIPVPGPGELL
                     IHVQACGVCRTDLHLIEGDLMPRSSGVVPGHQVVGTVVTGGSGGRFQPGERVGVAWLR
                     RTCGTCRWCRYGAENLCPDAEFTGWDADGGLAEYATVPEAFAYRLPTGYSDETVAPLL
                     CAGIIGYRALLRANLPRGGRLGLYGFGSSAHITAQIALAEGAELFVMTRGEANRTLAT
                     DLGASFVGEATDQPPEKLDSAIVFAPAGELVPVALGAVDRGGTVALAGIHMSDVPQMA
                     YERLFLERDLRTVTCNTRRDGEELLRLASRLPLAVHTTTYPFDKADQALFDLSSGAYA
                     GSGVVLMP"
     gene            complement(1301..1750)
                     /locus_tag="VH419_05275"
     CDS             complement(1301..1750)
                     /locus_tag="VH419_05275"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY7043061.1"
                     /translation="MPESDKVIRVAVDGSAGSRRALEWALDEARLRSCAVELVHVYAG
                     NDSSTEEAHRRAEDVIEAMVEQVAAAGAHHPQISWEVVRGEAAEVLVRASEHSQLLVL
                     GGHDLTGLRHSSQPSAADLCIRMAACPVVVVPIPHEPTTHSNLFTPV"
     gene            1953..2177
                     /locus_tag="VH419_05280"
     CDS             1953..2177
                     /locus_tag="VH419_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043062.1"
                     /translation="MPSKSANVKNEKQYEALKDKGMSKQRAARIANSPDSSSRGGKKS
                     GSGSSSSQGGTTAQKKKAGRKGGKAAAKRN"
     gene            complement(2203..3006)
                     /locus_tag="VH419_05285"
     CDS             complement(2203..3006)
                     /locus_tag="VH419_05285"
                     /inference="COORDINATES: protein motif:HMM:NF013715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEY7043063.1"
                     /translation="MLYLPWVLVAVWLVIWRVKFEKMEQTFGLLGLLLFAFAVALWHS
                     HPDWGRLADEAGSFGPPGPESWASYWYYAIALFGAAMTPYEVFFFSSGGVEEGWTPRD
                     LMEERANVLIGFPLGGTLSLSIAALTTVVLLPVGISVDTLGQVTLPVASELGVIGLIV
                     VLVGIFAATFGAGLETALSCGSTIAQYFGWQWGKFVRPRESPRFHLVIIVTLIVGAMV
                     LLTSVDPRPARTELNGIVDVCSKQTHTQIAWLETRMKQAAPQALVVAGG"
     gene            complement(2952..3347)
                     /locus_tag="VH419_05290"
     CDS             complement(2952..3347)
                     /locus_tag="VH419_05290"
                     /inference="COORDINATES: protein motif:HMM:NF013715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEY7043064.1"
                     /translation="MKKLLGITLGILTAIGGFVDIGDLVTNALVGSRFGMHLAWAVVL
                     GIVGICLYAEMSGRIAAESGRPVFDLIRERLGPRTALLALLGSFAVTALTLTAELGGV
                     ALAFELASASTTCCTCHGCWSLSGWSSGG"
     gene            complement(3344..>3789)
                     /locus_tag="VH419_05295"
     CDS             complement(3344..>3789)
                     /locus_tag="VH419_05295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043065.1"
                     /translation="RLRVRIAAVLIGVVILSGCAGPTTNDDAMRSQAARSANSATSEI
                     ETLKLAVTTQLDGNGWWRFTDVVVTDSETALDSINSTLASRQPPSTKTEAIRADTVTA
                     LGDAVDLAIDVRIAVRRHDESTLESLLPKLTQMSDRLGALEARAR"
BASE COUNT          686 a         1303 c         1253 g          547 t
ORIGIN      
        1 tgcgatgttc acatcgcaaa cccacgccgt gcaccgcgga cctgcgatgt aaagacggcg
       61 cctgggctgt aaacatcgca aacctcgagc cctcccggcg tacaagcacc acctcgacga
      121 ggtgacgcac gtgaccacga ccccactggt cgtggtcacg gcatgagcac gacgcccgat
      181 ccggcgtacg cgccgctcga gaggtcgaac aacgcctgat ccgccttgtc gaacgggtag
      241 gtggtcgtgt gcaccgccag tggcaaccgc gacgcgagac gcagcagctc ctcgccgtcg
      301 cgcctcgtgt tgcacgtgac ggtacggagg tcacgttcga ggaagagccg ctcgtacgcc
      361 atctgcggga cgtcgctcat gtggatgccg gccagggcaa ccgtgccacc gcggtcgacg
      421 gcgccgaggg cgacggggac gagctcgccc gcgggggcga acacgatcgc cgagtcgagc
      481 ttctccggcg gctgatccgt cgcctctccc acgaacgagg cgccaagatc tgtcgccagc
      541 gtccggttcg cctcgccccg cgtcatcacg aacagctcgg ccccttcggc gagggcgatc
      601 tgcgccgtga tgtgggcgct tgacccgaac ccgtacaggc cgagacgacc gcctcggggg
      661 aggttggcgc gcagcagggc gcggtacccg atgatcccgg cgcacagcag cggtgcgacg
      721 gtctcgtcgc tgtagcccgt agggagtcgg tacgcgaacg cttcgggcac ggtcgcgtac
      781 tctgcgagac caccgtccgc gtcccatccg gtgaactcgg cgtcggggca gaggttctcc
      841 gcgccgtagc ggcaccacct gcatgttccg caggtacgcc gtagccacgc gacgccgact
      901 cgctcaccgg gttggaacct cccacccgat ccacccgtca cgacggttcc gacgacctga
      961 tgtcctggca cgacacctga gcttcgcggc atgaggtcgc cctcgatcag gtgcagatcc
     1021 gtccggcaca ctccgcacgc ctggacgtgg atgagcagct cgcctggccc gggaacgggg
     1081 atcgggcggg tcgccagcgt cagactcacc tcgggcaccg gagccggtgc gtcgaacgtc
     1141 caggcccgca tcgactcgct catctcgcgc tgcccttcgt ccacatcacc cctcctcctg
     1201 agtatccccc cgcccttcca acgccgaggt ggcaggtttc gttgcgttct gccggcgtgt
     1261 cgcacggcga gtgccacctc ggtgaccgct gcgcgaagga ctagaccggt gtgaagagat
     1321 tcgagtgggt ggtcggctcg tgggggatcg gcaccaccac gaccggacag gcggccatcc
     1381 ggatgcacag gtcggcagcc gacggctggg agctgtgccg aagccccgtc aggtcgtgcc
     1441 cacccagcac gagcagctgg ctgtgctcgg aggcacgaac cagaacctct gcggcttcgc
     1501 cgcgaacgac ctcccaggaa atctgcgggt ggtgcgcgcc agctgccgcc acctgttcga
     1561 ccatcgcctc gatgacgtct tcggcgcgcc gatgggcttc ctcggtggag gaatcgttgc
     1621 cggcgtacac atggacgagc tcgacggcac aagagcgcag gcgagcctca tccaacgccc
     1681 attcgagtgc ccgcctgctg ccggccgaac cgtcgaccgc aacccggatg accttgtcgc
     1741 tctccggcat gtccatcacg cctctcgatg gcacgaagcg tactgactgc tcctccgact
     1801 cgaccacgac cgggcacgca ggtctagggc cgttcggccc gagccgccgt acggcagcat
     1861 cccgccgaag agtctccgac cggcggacgg ccgtgtgagc gtgtgtgcaa cggggtacct
     1921 cccgattgga gcacaacgag ggaggtgcac ggatgcccag caagtcggcg aacgtcaaga
     1981 acgagaagca gtacgaagcc ctgaaggaca agggcatgtc caagcagcgc gcggcgcgga
     2041 tcgccaactc gcccgactcg tcgtcacgag gcggaaagaa gagcgggtca ggtagcagca
     2101 gctcgcaggg tgggaccacc gcgcagaaga agaaggccgg ccgcaagggc ggcaaggccg
     2161 cggccaagag gaactgaccc gccagaccga tccatcgcag gttcagccgc cggccaccac
     2221 gagcgcctgc ggagccgcct gcttcatccg cgtttccaac catgcgatct gcgtgtgtgt
     2281 ctgcttcgag cagacgtcga cgatcccgtt gagctcggtt cgtgcagggc gcgggtcgac
     2341 gctcgtcagc aggaccatcg ccccgacgat cagcgtcacg atgatgacga gatggaagcg
     2401 cggcgactca cgcggccgta cgaacttccc ccactgccag ccgaagtact gcgcaatggt
     2461 ggagccgcac gacagggccg tttcgaggcc ggcgccgaac gtcgcggcga agatacccac
     2521 gagcacgacg atgaggccga tgacacccag ttcgcttgct accggaagcg tcacctgacc
     2581 gagcgtgtcc accgagatgc ccaccggcag caagacgacg gtggtgagtg cggcgatcga
     2641 cagcgacaac gttccaccca acgggaagcc gatgagcacg ttggcccgct cctccatcag
     2701 gtcgcggggc gtccagccct cttcgacacc gccggaggag aagaagaaga cctcgtacgg
     2761 cgtcatcgcc gccccgaaca aggcgatcgc gtagtaccag tagctggccc aggactccgg
     2821 tcccggcgga ccgaacgacc ccgcctcgtc ggccagccgg ccccagtccg ggtggctgtg
     2881 ccacagggcg accgcgaacg cgaacagcag caggccgagc aatccgaagg tctgctccat
     2941 cttctcgaac ttcacccgcc agatgaccag ccagacagcg accagcaccc atggcaggta
     3001 cagcatgtag ttgacgcgga cgcgagctcg aaggccaatg ccacaccgcc gagctccgcg
     3061 gtcaaggtga gcgcggtgac ggcgaaggag cccagcagcg ccagcaaggc ggtccgcgga
     3121 cccaaccgct cacggatgag gtcgaacacc ggtcgaccgg actctgccgc gatccggccg
     3181 gacatctcgg cgtacaggca gatgccgacg atcccgagaa cgacagccca ggcgaggtgc
     3241 atcccgaagc gagagccgac gagtgcgttc gtgacgaggt ccccgatgtc gacgaagccg
     3301 ccgatcgcgg tgaggatccc gagtgtgatg ccgagcagct tcttcacctg gcacgcgctt
     3361 ccagagcgcc gaggcggtcg ctcatctgcg tcagcttcgg caggagggac tcgagcgtcg
     3421 actcgtcgtg tcggcgtacg gcgatgcgta cgtcgatcgc aaggtcgacc gcgtcgccga
     3481 gcgcggtgac cgtgtctgct cggatggcct cggtctttgt cgacggcggc tgccgggacg
     3541 cgagcgtcga gttgatcgag tcgagggcgg tctcggagtc ggtcacgacg acgtcggtga
     3601 accgccacca gccgttgccg tccagctggg tcgtcacggc gagcttgagc gtctcgatct
     3661 cggacgtggc cgagttggcc gagcgggcag cctgactgcg catcgcgtcg tcgttggtcg
     3721 tgggtccggc acagccggac aggatcacga ccccgatcag taccgctgcg atccggactc
     3781 gcaaccgct
//
LOCUS       DATBFZ010000177        36096 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_4054041, whole genome shotgun sequence.
ACCESSION   DATBFZ010000177 DATBFZ010000000
VERSION     DATBFZ010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 36096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36096
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4054041"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(197..4489)
                     /locus_tag="VH419_05300"
     CDS             complement(197..4489)
                     /locus_tag="VH419_05300"
                     /inference="COORDINATES: protein motif:HMM:NF039478.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6519 domain-containing protein"
                     /protein_id="HEY7043066.1"
                     /translation="MNGGDISRSTFVATRHYTGVRMQQGRVQLDADWNELVDIERHLE
                     QTEALDVIGPTGVPKATAGFELSLAPDGTDLLLSPGRAWVGGTLCELEADTTVVTTVA
                     GSALTVAAIVLDGAELAPHDWVDLRGDASAVTLRVIAVEAAQSVVTVDGTPDTNALGE
                     HLKLSRRRSYATQPDLPQAPFVTQADANTPPTPTLPAGMYLAYLDVWQRAVTALEEPD
                     LREVALGGPDTTTRSQTVWQLKLLTLEGAPAGLTCGSDLSQWTPTLAEPSGRMAARAE
                     LDQTTQNLCTPTAAGGFAGLENQLYRVQVHDVTDDGRPIVLWSRDNGSVVTAWLGNGA
                     GAGELTVADIGKDSVLGFGASQWVELTDDVHELSGAPGTLVNLLDAKGTTLTFATSGP
                     DAPTGSTTFTDFHGTPKARRWDSPGAVTVDFDTWIALEDGVEVLFPSGGTYRRGDYWL
                     VPARTVTGGVSWARDSAGNPLARRPDGVAHAWGRVALVSVPDNAPATVIDCRDLFPSL
                     TTLAASDVAYTGTACVIPGADTVQEALDQLCKEHDLRRHHRLLHGWGIVNGLQVHCAA
                     PSQVIVQPGTAIDAEGNDLDVTASTPVDLLDLAAAVPDAVDGDGNGDVCLLLTSDPEL
                     QIGFAVEKYDKTQDEPPALLQGTLWSDFYRDNIQSLHDFIHEKLTPGPDDGVTSPARQ
                     LLSALTNVAAQAVNQTSGQNLFISPSEDKLLQQFYVDLRQRLQSDTFCAMFANARTPA
                     AYPTTWSGMDVIFGAGSHARLRVRPGAAEAYTVGPGTNPLQPTTVINRYDLTSGTMTA
                     RIDPVAGKELAAGEKPVSGYGPVTDVAFSPDGRQIYVAVPASNEDNTFFRVGDIRPNG
                     IDWRPSVTICGVKFVTLATAAAIPGAVYAIGLKKVTVNNKVQWQGAGLYQINPDSVDP
                     TMSPMPFPASFNPVGHLSVLPDGRAVATSAAPGQPVTSYSSVFQFTLPNPPPEGWQIA
                     LPVSGNDDVALIDVGGAALIYTVVSQNGRSFVGNEFGNGAAVTGAPVQLQTGTGAVRL
                     LPVGDHVVATSSDDYLLRMIDPRGGQLVPEFKLPLQVGPIAIAASDSTKRVYVLNYVS
                     NTLWEGNDELFVPTTTFPFDDLTAYRKAMVEAFADLLYGFLQYLKDGLFDHFLVNAPE
                     VTGNEKLYLAAVSIRSNQVYRVCNFTRRRYVKSFPLIGYWLSVVPFMPMLKQRFAEVA
                     CTVLPDLLKPYNVQTGATGSDRLSVEMLMQAVAWAQSTDVPGRVREVRNKSNVASGAA
                     GLALRTLRPAPRPIGGRALVATDLVGKQSDAAAQIATSKGLTVETRAFDPSQGAGSVK
                     DIAGVFRSAQPGDQVTLFTDDTGQVRYYEVVKPAPTIDIGPNTPADVITLQGQLTQAQ
                     TQLNQTLIQLTTLQTQQTQRDAELAELRSQLGTLSNAHDQLRNQIGNLNPPQ"
     gene            complement(4502..6619)
                     /locus_tag="VH419_05305"
     CDS             complement(4502..6619)
                     /locus_tag="VH419_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043067.1"
                     /translation="MTLVDELWALVPAVYRQRDAQSPQSGTLRALVEILGEQAQIVAD
                     DIAQLYDNWFIETCDPWVVDYIGDLLGVRVLHQVGPRTQLPRAYVANTLAYRRRKGTA
                     AVLEQLARDVTGWPAHVIEGFTVVGTTQNVNHLRPQNQQGINLHDAAGLERIGTAFDP
                     SARLAEVRLPPAGISNVPDIGLAVWRLQPFRVERATARPVSDPPDGRYHFDPLGLDVP
                     LANPPQPESTITSLATEQHVPGTLERRLLFDELEGLRAGTVEAPVFFSADPVVRVYAD
                     TGAGLAEVPVAELTAADLSDPPPSVTTGWRRPGSPLTAAIDPVLGRLAFRDGVVPSLV
                     EVDYTYLAAGPIGAGAYDRSGAVTSDLLARASWWRAVGTDLPAGPSVVTTASEAVDDW
                     NAQPAGTVGVIALLDSRTYAGDLALTIPAGSELLVAAASWPQVADPQPGDVAEPAQIA
                     LGVTRPHILGSVTITGGAPPDDSVTRGRMLLDGLLVEGAVEVASGDLGELHVLHCTLP
                     TAGVQVAGDDDLSVVVGEAVVGPVTMAGSGPTLSVSQAVVDGDVGAAEVDVTLDQVTV
                     LGDLAARSLVASDAIVTGAAVVERKQAGCVRFSYVGDGSATPRRYRCQPDLALNPSVG
                     TVDPVAVRARVRPVFASDRLGDPAFGQLADRCAVELTAGSSTQSDMGAFASLQRAQRE
                     ANVGVALDEYLRFGLTAGLIHAT"
     gene            complement(6616..8448)
                     /locus_tag="VH419_05310"
     CDS             complement(6616..8448)
                     /locus_tag="VH419_05310"
                     /inference="COORDINATES: protein motif:HMM:TIGR02243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative baseplate assembly protein"
                     /protein_id="HEY7043068.1"
                     /translation="MAQKVIGNTGDKVTGMQQGGLQFFTWPPTEWPDFVVSEDPKTVD
                     LDTTYPALVPGGLVVLTKAGQSAVFVIDGVQDTSRSDFALSARVTALTLHTSMSSAYA
                     GSTPREVSVLAGSEQLSLVGAPLAAPVEGKVIQLGGPVTNLPAGRAVLAVGQSPIMRV
                     ESASLVLTTSSGGQQNLEVGDELTITGPATSVGSSSVWPVQHGADTGTVAVSPGSATF
                     LSGATGSAPVVELAYVDHQDGLTLQLQQSLHGSFDRSTVRFAANVAPATHGETKREIL
                     GNGDASATFQSFTLAQAPLTYVSSTTSGVTSTLQIWIDGTRWREVGSLFGSSPKDRVY
                     TVRIADDDKVTVGFGDGITGARLPTGTQNVSAVYRVGTGSAGLVDAGQLSMLMTRPLG
                     VRGVSNPQAPTLAADPDPPELVRRNAPRTALAFDRVVSLLDFEDFVRAIPGYAKAQAA
                     ELLVNGTYAVHLTVAADGGGAVTDADRADLLATIQSNGDQRLLVAIDSAELIRFYVAL
                     TVVVAPDRAPGAVIDAVRAALLAQFGFDGRDLGQPVTATEVMAAAQGVPGVVAVNLTL
                     LDTTPGGGRQSILAARGARGDGKTVAPAQLLTVAGDGITVVAST"
     gene            complement(8477..9331)
                     /locus_tag="VH419_05315"
     CDS             complement(8477..9331)
                     /locus_tag="VH419_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043069.1"
                     /translation="MSDVDATNVAPLSDCSCCDGVHAATPRSTDNPPGARRLDYRVGT
                     AGSFLESMRARLSSTLPALRSRGTDDPTIALMDAWACVLDVLAFYEERIVTEGYLRTA
                     TEGLSVTALARAVGYERSPGRAAATWLQFTLEEGVGAPAEVPIPTGTKVASLPGPGQT
                     PQTFETVADVGARPEWNAIGVAQAGSPLDGFPFGRLYVEGAATSIRAGETLLFRGPGG
                     AFVLHTIQRVTPLPTGITEVVWGVAVPASYDEVHVLHQRAAVFGASAPDWRTLPDSIR
                     DHYKPIFI"
     gene            complement(9354..11690)
                     /locus_tag="VH419_05320"
     CDS             complement(9354..11690)
                     /locus_tag="VH419_05320"
                     /inference="COORDINATES: protein motif:HMM:TIGR02243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative baseplate assembly protein"
                     /protein_id="HEY7043070.1"
                     /translation="MSAVVNPPTVQFRCRNPRRAEAVRESTTLNGIDFLEVSDDQTAL
                     DVHVLRDLAASPALNAANVRVEGGVRATGITVLTATPTAADVLTVTVDRPGDFSPYVL
                     RLVDPVSPDQPAAGYDKRLSDVTFSFKAGCPSDLDCAAVVSCPPVLADEPALDYLAKD
                     YASFRGLMLDRLATTLPGWTERNAADQQLMLVELLAYVADRLSYFQDAVATEAYLTTA
                     RRRVSVRRHARLLDYRMHDGCSARTFVHFTASTGLTLHAGRAVQAGPDEDAVVFQLLH
                     EVELRAANNGIPLYTWGDDLCCLPAGATRATLDATDGPELAVGDFLLFEESTGPTTGD
                     PADADPTHRQVVRLTDVTPDNDPLTSTSLLEVAWSSRDALAFPLCVTNIATVDGVHQH
                     VVCGVARGNVALADQGLSGQATLPLADDPTDAPGRWRPRLPEGPVTQVVDYDQHAPAA
                     ALLAMDPRTAHPVVTLDDGELEWTPDPAGDLVDAAATAPVFVLEIEDGGETRIRFGDG
                     VSGMEPARGAELTATYRTGNGTPGNIPGEALDRLREAESGVDQVRNPLPAQGGVDPEP
                     MEQVRRLAPQAYRVQERAVTAADYSTVTAEVPGVQRAATNLRWTGSWYTAAITVDQIG
                     GGALAPDLVASVSDVIQTQRMAGVDFELNQPVPLPLELALDICVRPGYFATQVESQVL
                     DVLSSRVLPDGRRGFFHPDNFTFGQPLYLSQVYAAVLTVPGVAQVNATALHRFGKTPG
                     HELADGVLVPHGLEIIQLADDPNFPERGRLTLNVVGGL"
     gene            complement(11687..12046)
                     /locus_tag="VH419_05325"
     CDS             complement(11687..12046)
                     /locus_tag="VH419_05325"
                     /inference="COORDINATES: protein motif:HMM:NF016829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HEY7043071.1"
                     /translation="MQVDYPLHVDRRGATALTDQDGHVRDLIEQLLFTAPGERVNRPT
                     FGTGLLQLLFLPNSEPLAAATQASVQGALAQWMGTLITVKDVVVVADDATLVVTVQYV
                     VNRTGEDTTAQFVRSLA"
     gene            complement(12046..12363)
                     /locus_tag="VH419_05330"
     CDS             complement(12046..12363)
                     /locus_tag="VH419_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043072.1"
                     /translation="MPGYLLHVGATVLCMHGGQAQATVPIPRVLVSNQPVVGQTGPYV
                     VAGCSLVPPPIPPCVSAQWVVAATRVTSGGVPLVLQDSTAVCTPTGTGLQVLVTQLRV
                     KGT"
     gene            complement(12364..12864)
                     /locus_tag="VH419_05335"
     CDS             complement(12364..12864)
                     /locus_tag="VH419_05335"
                     /inference="COORDINATES: protein motif:HMM:NF016595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HEY7043073.1"
                     /translation="MNSPFLGKYRGVVTDIEDPLMIGRIRATVPDVLGSDESGWAMPS
                     LPFAGSSVGFFALPTKGSGVWIEFEHGDPDYPIWSGCWYGSAADVPPLLLAPPYKKVM
                     VITPAGHSVTLDDTPGVGGITLETADGAKFEMTSLGITIDNGKGASISMQGPKVSVNN
                     GALDVI"
     gene            complement(12861..13976)
                     /locus_tag="VH419_05340"
     CDS             complement(12861..13976)
                     /locus_tag="VH419_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043074.1"
                     /translation="MLESLLGVRVLIWTGGLVPTPHFELADALESIQVTNDTDAGDGF
                     QMTFNVGKDTFGGYDVLDGGALELMNRVWIAVVLGVVPEVLIDGVITRHDLRPSDDPG
                     RSTFTVTGTDVSLELDLEEKNAPFSNQPDYVIVSQILLSYPQYGLVPMVTPTTDFPIE
                     MDRIPRQHETDLAFIRRAADRNSFVFYVQPLTLGVNTAYWGPVVRAGIPQPAITLGLG
                     PDANCNSLSFSNDGLAPVGASGSFLEPITKVAIPIPALPALRLPPLAASPTPTSRTTL
                     LRESGNESPIQAALASAAAATRAPEAVTGNGEVDTARYGSVLRARGLVGVRGAGRDYD
                     GFYFVRSVTHSIRKGSYRQAFTLSREGTGSLLPAVLP"
     gene            complement(13979..14278)
                     /locus_tag="VH419_05345"
     CDS             complement(13979..14278)
                     /locus_tag="VH419_05345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043075.1"
                     /translation="MFDKTSRYAGIDTVTVTVSDPDGDTRDIRYVRRRFVPAAGSHTT
                     LVEHRMAAGERLDLLAARYAGDPLQFWRLCDANDVLRPEDLEVVGRVVAIAMRIG"
     gene            complement(14280..14939)
                     /locus_tag="VH419_05350"
     CDS             complement(14280..14939)
                     /locus_tag="VH419_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043076.1"
                     /translation="MTGFSGSPRVTRGALIGLDLLNPLASVIVFQYNPEQVTRRLMAR
                     TAESGGSQAEQLRLKGPPEETLTFDIEIDAVDQLEQGDSTAVQLGIAPALASLELMLY
                     PKSALVIANEVLAALGIIEVIAPDAPLAVLVWGPRRVLPVRLTEFSITEQAYDPALNP
                     IQAKVSLSLRVLNYDDLGLLSAGGALFMAQQIAKEVMATVNSVSSLGSFSAAASIQLG
                     G"
     gene            complement(14936..15142)
                     /locus_tag="VH419_05355"
     CDS             complement(14936..15142)
                     /locus_tag="VH419_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043077.1"
                     /translation="MSTTIRVRIDSVVLPSAADDRVASDVMAGVLVAGGLPEDLGPRV
                     AERLVADLRAAVEGGGTSRRREPR"
     gene            complement(15139..17058)
                     /locus_tag="VH419_05360"
     CDS             complement(15139..17058)
                     /locus_tag="VH419_05360"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY7043078.1"
                     /translation="MTAVTHDEWTELNQRHLSAAVAEVRAMLERQLASAADAEVDSRP
                     TDVADATSTTSTTTTAASTTDTEPPGALDVVCTGFGLSAFERLVLILCAGVELDSSFA
                     DLVSKVSAGRQGHPTFGLALAALPGAHWSALSPGRPLRRWCLVELHGSSPVTGELRID
                     ERVLHVLAGVGDADQVLGGLVHAEPVPEHLVDSQVQVAAQLVDTWRAAPEVVPQLLGR
                     GTAAKRAVAADAAAQLGARLAVLDARGLPSDPQDLARLATLTDREAVLAGRILCIDAD
                     DIEQTASTGLFLDRAEGLVVLCARDPIDLHARPALGLDVKRATATEQASLWRELRGED
                     QETTAVSEQFDLDPQVIQTLARTARPGELWARCRASTRPRLGPLAQRVDTGAGWAQIV
                     LPAAQLDALHRIANQVRHRKTVFEAWGMRGASARGTGTTALFAGPSGTGKTLAAEVVA
                     GELGLDLYRIDLSAVVSKYIGETEKNLRRVFDAADEASAVLLFDEADALFGRRTEVKD
                     SHDRFANIEISYLLQRMEQYHGLAILTTNMQEALDPAFQRRIRFVITFPFPDADRREQ
                     IWNGVFGPEAPLADFDPRRLARIGLTGGSIRNIAINAAFLAAANGRRVTMDHLFQAAR
                     DECVKLDQPWPGTPS"
     gene            complement(17055..18278)
                     /locus_tag="VH419_05365"
     CDS             complement(17055..18278)
                     /locus_tag="VH419_05365"
                     /inference="COORDINATES: protein motif:HMM:NF025431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4255 domain-containing protein"
                     /protein_id="HEY7043079.1"
                     /translation="MSNFLAVATVTAAIQQLLGQPAADDVPGATVSTDRPDTKKNQTA
                     PQINVYLYHVSPDPFARAVDLPTRRSDGTQVQPAQAALDLHYLLTFYGDDKELEPQRL
                     LGSAVRTLHTQPVLTRELIGDVVAAANANPAIHPSLVTTDLADAIDVVRVCPMPLDLE
                     QLSKLWSVFFQTPYALSTAYTASVVLIEDASKVSTGPPVLSPVLSVFPIPKPTIESVT
                     VSGSPFAPITANRVIEISGTQLKGDETTARLGGVDLTPTSTSATSVVVDLASAPAGAL
                     RAGTVAVQVVQARLVGDPPEPRGQVSSPVYPVQLHPRVVSVAHAGGTLMVTSDVTVST
                     RQRAAVELVNTATDAVVALLNVPDRTDDGTSLGVSVAGVAGGTYGVVLLVDGAPGPVT
                     RDGAGHITNPTVTLP"
     gene            complement(19131..19790)
                     /locus_tag="VH419_05370"
     CDS             complement(19131..19790)
                     /locus_tag="VH419_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043080.1"
                     /translation="MDALGPAAVLDLWDHGGRLVPAERALMALSLAGIDAGERTVGER
                     DAALLALHAATYGGSLAGVTDCPACGESLDVDVPTAHLAVDPPTAGARSIEIADDVLS
                     FRLPTAADVAAARDADELFERCVNATATAGVRDEIEALMALSDPGAELEVALTCAACG
                     EEWIEVLDPVRFFWSATVGAARSVSEDVGVLARAYGWSEREVLAMSGRRRELYLEALN
                     R"
     gene            complement(19790..20317)
                     /locus_tag="VH419_05375"
     CDS             complement(19790..20317)
                     /locus_tag="VH419_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009073193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEY7043081.1"
                     /translation="MAQFTVNPTRFDPYKNFKFRVKWDGRYVAGVSKVGALKRTTEVV
                     KHRDGGDPSTSRKSVGRTEFDAITLERGVTHDTEFEKWANKVWNFGAGLGSETSLKDF
                     RKDVILEVYNEAGQLAIAYKIFRCWVSEYQALPDLDANANAVAIQHLKLENEGWERDQ
                     EVPEPTEPTFTNPPT"
     gene            complement(20330..21961)
                     /locus_tag="VH419_05380"
     CDS             complement(20330..21961)
                     /locus_tag="VH419_05380"
                     /inference="COORDINATES: protein motif:HMM:NF037565.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath C-terminal domain-containing
                     protein"
                     /protein_id="HEY7043082.1"
                     /translation="MPATYTYPGIYVEEVPSGVHPIVGASTSDTAFVDYFAQGPAGVA
                     TRVTSLAQFTTVFGLVDQASEASYAVASYFLNGGQVAWIVRVVPGFDPAAPDEDMLAT
                     IELVQGSPGTGALVVQAASPGAWGSQVEVAATETVPAEPDSFNLFVRQVVTTGSDSRI
                     VASEVYRGLGVTPGSANDAVAVVNAGSALIRLSAPGGGSAAIPNLSTPTSSGDPLDTA
                     WRALATPTTLPDVIDGTSLEATDDFVSALTAAVTPGSSPLDHVDPFHINLLCVPATSR
                     VDHGTRKSLMDAVVAYAVTRRAFAILDPPPSTEVGTTDKMRTWLLGSEAPAASPNGAV
                     YYPRISINDPLAGGRPRELGPSGMMAGVYARTDASRGTWKAPAGIDATLAGATLVSAL
                     ADSDSAVLNPLGINALRTLPVIGNVAWGARTLVGADQRASEWKYVPIRRMALYIENSL
                     VDGLNWVVFEPNDEPLWAQIRLNVGSFMQNLFRQGAFQGTTPKDAYLVQCDSTTTTQG
                     DIDRGVVNILVGFAPLYPAEFVVIRIQQLAGQSQS"
     gene            complement(21971..22693)
                     /locus_tag="VH419_05385"
     CDS             complement(21971..22693)
                     /locus_tag="VH419_05385"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY7043083.1"
                     /translation="MARAPLVSCIMPTCDRRRFVRRSLRYWRRQEYAFRELVVVDSGI
                     EPIVDLLPRDPSVRYQRATPGLSLGAARNLACTAARGAYIAHWDDDDWMAPSRLRHQV
                     GALDASTAQVCGLGTLTYLDRGRARAWRYTPGPHDPPWLAGGTLVYRREAWEGHPFRD
                     VTVGEDGAFLAGLAPDHLLALPDEGWYVAALHAGNTSSRPVGDDRWSPVDPRDLITRM
                     RHLTTCPARQRTARSDPVETLH"
     gene            complement(22730..23518)
                     /locus_tag="VH419_05390"
     CDS             complement(22730..23518)
                     /locus_tag="VH419_05390"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HEY7043084.1"
                     /translation="MDRSVYSSGPREAPTKVADPRLSLLGTWQLTVGGRAQHLPLGAQ
                     KLIAFLSLRGRSDRAHLVGALWPDASEEHAHGSLRSLLWRMRKTRLGLLDATDSCVAL
                     SDRVRVDVTDVIDDSTISIRGGVPDLDGTLPAHLLDGELLPGWYDDWVLIERQRIRQL
                     RLHALEALALTLARKGCFALGLEAALAAVRAEPLRESAHRTVIDVHLLEGNVVEALRQ
                     ADYCRRLLREELGLDPSPLLLATLPACATHQRLHQERSWSSSIA"
     gene            complement(23892..25589)
                     /locus_tag="VH419_05395"
     CDS             complement(23892..25589)
                     /locus_tag="VH419_05395"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY7043085.1"
                     /translation="MDRRVCIGVTVHAQPAMLRETVQFLHLHTDLSADLVVLPDGPDA
                     ETRAALSGDVELSSLTQWPTAEARGTAAAFNRLAAATDAAVIVLLESGSLVGPRWLEL
                     LLAALKTSGCGLAGPSTNLGWNEQALGWSPIDPAASASVAAPPERLAVVRAMSARTLQ
                     RFGREVRALGPLHSLGDFCYAVRREVITAIGDADEGYGLGPCWEMDYNIRAARAGFTG
                     VWVCGAYVHRSPPTARRTAHETAHGEASRHRYQDQFCGLRLAGRTESYEPHCRGDACE
                     HFAPPHLVNPHARRRDSRERRRTPTGPRTAVAPPVRAVPAPSTGAPLVSCVMPTRGRA
                     DFALQAMHYFQQQDYRNLELVVVEDGDRVLAASLPDDPRIRLVSSGTVRSIGVMRNDA
                     CARARGEIMILWDDDDWHGPSRVSRQVAPILAGQADITALRDVPLLDVAAWRTWRWSS
                     DLHRHMLALDVLGGTLAFHRSVWEHSARYPDDSLAEDAAFVRQAVRAGARLEALDATG
                     VYVYVRHRANSWHLECGQSVDPHGWMAIDEPPLPAPDRAFYQSLAGVGSRGSQREGR"
     gene            complement(25748..26560)
                     /locus_tag="VH419_05400"
     CDS             complement(25748..26560)
                     /locus_tag="VH419_05400"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEY7043086.1"
                     /translation="MSARRGASTSASTTEDGPATASRLPGYPPLIEVRVGRHSFRVVD
                     DEFAAFWERVTDGDWEPHSFAVIDHFVSSGLTFVDIGAWIGPLSLYAAHLTDRCHAIE
                     PDGRARACLEANIALNPPLAERIVVHPVAIAERPGVARLGSITSAVGGDSMSSLLFGD
                     ADASWPVECVTLEAFVSTLPPGPLGLVKIDIEGYEVSVLEGSRSYLAASHPPLYLSVH
                     ASFWDEPRPIMERLLDLLSTYRELLTPALEPIAPDDLLDDDHLSGLFELVAV"
     gene            complement(26517..28418)
                     /gene="iolD"
                     /locus_tag="VH419_05405"
     CDS             complement(26517..28418)
                     /gene="iolD"
                     /locus_tag="VH419_05405"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR04377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016823 - hydrolase activity, acting on
                     acid carbon-carbon bonds, in ketonic substances [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HEY7043087.1"
                     /translation="MEFYPSEKKQRLTLAQAIVKYLQVQFSEYDGEQERFIQGIWGIF
                     GHGNVSGLSQALVEYGQDLPYHQPRNEQSMVHASTGFARAKRRKATMACTTSIGPGAT
                     NMVTGAATATICRIPVLLLPSDYYASRYGGVVLQGIEHLSSEDMSVTDVFRVVSRFYD
                     RVTRPEQILTSLPEAMRIMTSPADTGAVTLALPQDIQGYAYDYPTSFFEPHVWRIERR
                     LPDPVRINEAIELLESAKRPYIIAGGGVHYAEAWDELNEFATAFGIPVGETHAGRGAL
                     RDGSANPLSMSGTGLNGTPSAAEVAAEADLVLCIGTRLHDFVTGSSSAFHDPGVRFVS
                     VNVNGRDAYKLGALPITADAKLALKALTDAGRAAGVSVNGDWVAKAQRLAGEWETTKR
                     EQVYVDRPGEDMTQAQVIGVVNEQMQDGDVLVCAAGTVPSDVTKLFDAAGGRTLHLEF
                     GNSCMGYDIPGAIGVRLALGGGEVYVLLGDGNYQLHPMELVTAMQERAKLTVLVSVNY
                     GYQSIHGHQRSYVGHSLGNEFKVRDAATGEIDKGEYLEIDYVKNAESIGLTAVAARTP
                     GEVRDALKAARDRDGSTLIACYVDKSYAPPGSGVWWEVIGAEVTNDATTRSVVDEREE
                     GRVNQRFHY"
     gene            complement(28449..29276)
                     /locus_tag="VH419_05410"
     CDS             complement(28449..29276)
                     /locus_tag="VH419_05410"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY7043088.1"
                     /translation="MARRVLITGITGNLGRKAVAALRALDDIVVIGIADRPADVDLGV
                     EVALADLSVVDASWERYFDDVDVVLHLAADMRPTAAWDSVVANNLNLSLLVLDAAERH
                     GVDRVVFASTNWVLGGHRFRRTRLTPSTPPRPVNAYGMSKLVIERAGAVLARRGVSFL
                     ALRIGFCQPGENHPGPHMPFGLWGQQMWLSNDDWRQAVQLACTVPFEGFAVVNVMSDN
                     VGSRWDLSETARVLGYRPQSRHRPRLSVGRRLTDVGARFRDSMIPVRTEAPLFGARW"
     gene            29371..30396
                     /locus_tag="VH419_05415"
     CDS             29371..30396
                     /locus_tag="VH419_05415"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY7043089.1"
                     /translation="MRRPTLDDVARQAGVSRTLVSLTLRGLAGAGDETRRTIFAAAEE
                     LGYVPHAGARNLASQSSRAIGVLLHDLHNAFFADVVDGLHASMRETGFHVLMASGDEL
                     QLERSVLDTFRSYRMDGAVLIGPEVREREIEAFGQAIPTVVVGRPVRSKAVDVIVSDD
                     RKGAMLAVDHLHELGHRDIAHLDGGTVPGGANTRRSGYVEAMRRRGLARYVRIAGGGF
                     THAHGADGVDQLLRRHRPPTAIFAVNDLVALGAMQRLAELGLTTPGDVSIVGYDNSSL
                     AAQRRVGLTSIDQPRFEIGMYAAALLQERFAGRHQSARRLLAPKLVERETTAPLDDHQ
                     LIRKGAE"
     gene            30393..31328
                     /locus_tag="VH419_05420"
     CDS             30393..31328
                     /locus_tag="VH419_05420"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEY7043090.1"
                     /translation="MTLTDTTTRDALGRLLADGYVVIPNLLDAATTENLRARVRVLLD
                     QERAHPFDPGPDAPEPDDDQLSWYAHIWDLDDDEKTRLSQRLLLREHDEFDTPWPVSN
                     ADVCISFIHIPTLFDRGRSQRIFNLINKDAAFAPLLEHPIVLSIVEDQLGLDAVLLDV
                     SVNAVGPQSSSGGWHVDSPLTQVPEPFPNFTLSMQTAWMLDDFHVENGATHVARGSHL
                     TLRQPPRGRGDVDDEVVLEGPAGSVAIWLSQTWHRHGENSTDAMRRGVIVQYGRCWVK
                     PFVDLRTPMTAAHAAELSPRLRYMMGCNANAPVRG"
     gene            31336..32475
                     /locus_tag="VH419_05425"
     CDS             31336..32475
                     /locus_tag="VH419_05425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043091.1"
                     /translation="MTRSGRLFASYAGFTILAWYLSAMGAVLPPLRDEAGNWAGVYAL
                     LPGAVLFGWGLITLARGSRSTSDREPNIAVGLTVLTISIGVMGVTGAPAISLIGAAVA
                     AIAAAHVNRTLPGMLATASGGRPVQPVLMRANAFSSVAAIIAPLAVGASLAIGAGWIT
                     GYVVPIAIGAVLVCALAMRAGGPERSFETMDPAPLPPFRHWWRACAVLGVAVVVEFCF
                     TYFAVTYLHDERGLSKAAAAAGGAAWGIGMAIGRFAFSVRQPPPSIVPSAAVILAGFV
                     LFWGVPNSAVAIIGITIAGLGVAPLYPSRIIILMERFPGATHEGSKRAALASGWALLV
                     APALMVSLRAASNVRVAYLVVPVLLVLLVALASPRWEHSRHLVAA"
     gene            complement(32685..33068)
                     /locus_tag="VH419_05430"
     CDS             complement(32685..33068)
                     /locus_tag="VH419_05430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043092.1"
                     /translation="MTDSQPSPVPPTGSPGPLDAELVGAWETSHWLDSTGSQQIKRTY
                     LFSPDGHYDYALGMCQGSNDCTIKDRASGDVQAANGMLTLTPQTGSQQGPQSWPYVVD
                     RDPVVGDVRLVLTLPDGQEDIFYRP"
     gene            33130..33294
                     /locus_tag="VH419_05435"
     CDS             33130..33294
                     /locus_tag="VH419_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043093.1"
                     /translation="MSGTDSGTGATVSGKDGTNEADGVDALLPHAAESESTSAASAPV
                     PTMRGACPRP"
     gene            complement(33305..>36096)
                     /locus_tag="VH419_05440"
     CDS             complement(33305..>36096)
                     /locus_tag="VH419_05440"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HEY7043094.1"
                     /translation="DENLQPLGTDRKNAIKALSARYRAAIAAIQAGRVICRQTATTVQ
                     QRVATLRDIARNIERGNYFLDELGDQVRDLRLSTHVDPRRGVLASAAMPVFADPIVIG
                     AERYVDAGIREIIPMEIAVRNGVTDIVGICCSSLELPRVDDMSNAGLAEVGMRELTQI
                     ALQEVTVDDIAAARAKNISCTVIAPTFDVHGGTEVNESLIEISSAYGWMRAGDEMQPA
                     LDSARRDFRRLSDLIATFRIRSYSLERYIGENDWFVRAVETREPVATVRTYRWIIREL
                     LARRTELGLPLHPQAQRWWRNWAHEFRPLGPFGTVSVWSRLSAFSNNGTEAWVADGVL
                     DPNTYAPDAGSLIDAGTDQVYWMVRGAVFLASNETEHSTTRTPTLAVPHATFTDLPRI
                     PRGSHLMAEQNTPTQVWIVRNGTRYRSTPALVTASGMAGQPVARVPPGGLAQIPDGGL
                     PYWLGGLYVSDNLRHPVDRWEPTPQVEGSTSSTAVGLTNKTAQPVVVSGLTITTSQDG
                     GGTTAFSVTTPLPLTVPPNSFLWVNAQFRPRQPGPITGTVSVTCNDPAVGQFAVPLAT
                     SATPLGPHGVLQVSPSSLDFGVVRAGRPAGQSVTLTNVGARSLGIAELRIIDSSPDGQ
                     FAIPLSFPGQLTPGQSQPVYLSCTPTTRGPLTATLSAHVSSATDTPYPFEQRVDVPLA
                     ATAQAPVVLLAGRALPPAGPPLGPPLHPGPGFPLPHPGGAQLLPELTLLDFGSAAPGT
                     TVTRTFWIRNVGDAPLSVSSVDTTGPAAFGVPNLSIFPAVVNPGGELAVQASFGASAV
                     AGAPAAATLVIHSDDPLRPRAELAVAGRTVGGHLTMQPAELLTLNSGAPPTGELVINS
                     DGTDPVTLQKIGVDDHDFALSGVPAPGTTLGPGASLTIDVTYTGMTPGRHDGSLDIAH
                     DATASQSRIFLRAFT"
BASE COUNT         5463 a        12383 c        12785 g         5465 t
ORIGIN      
        1 cccgctgaga ccgagatccg cgaggttcgc tggctctcgc ccacgccccc ggatccccct
       61 cgatcacaca tgccccgtcg ttctgacccc atcctcagca ttccggtcac gtaaacgcac
      121 gccagacggc ggatcacgtg acatgaacga cctcagtgag actgcgaggg cgctcaggga
      181 tgtcgccccc acggcctcac tggggcgggt tcagattccc gatctggttg cgcaactggt
      241 cgtgagcgtt tgacagcgtg cccaactggc tccggagctc ggccagctcg gcgtcgcgct
      301 gggtttgttg cgtctgcaac gtcgtcagct ggatgagcgt ctggttgagc tgcgtctggg
      361 cctgcgtgag ctggccctgc aacgtgatga cgtcggctgg ggtgttcggt ccgatgtcga
      421 tcgtcggcgc cggcttgacc acctcgtagt accggacctg gccggtgtcg tcggtgaaga
      481 gcgtgacctg gtcgccgggc tgggcgctcc ggaacacgcc ggcgatgtcc ttcaccgatc
      541 ccgcgccctg cgacgggtcg aaggccctgg tctcgacggt cagacccttg gaggtggcga
      601 tttgtgcagc cgcgtccgac tgcttgccaa ccaggtcagt tgcgaccagc gcccgcccac
      661 cgatcggcct cggcgccggc cggagggtcc tgagcgccaa cccggccgca cccgaggcca
      721 cgttcgactt gttgcggacc tcgcgcaccc ggccaggcac gtccgtcgac tgcgcccagg
      781 ccaccgcttg catgagcatc tccaccgaca gccgatcgga tccggtggcg ccggtctgca
      841 cgttgtacgg cttcaacagg tctggcagca cggtgcaggc gacttcggcg aaccgttgct
      901 tgagcatcgg catgaacggc accaccgaca accagtagcc gatgagcggg aagctcttga
      961 cgtagcggcg acgggtgaag ttgcagacgc ggtacacctg gttcgagcgg atgctcaccg
     1021 cggcgaggta cagcttctcg tttccggtca cctccggcgc gttcacgagg aagtggtcga
     1081 acaggccgtc cttcaggtac tggaggaagc cgtacagcag gtcggcgaag gcctcgacca
     1141 tcgccttgcg atacgcggtc aggtcgtcga acgggaacgt cgtggtgggc acgaagagct
     1201 cgtcgttgcc ctcccacagc gtgttcgaga cgtagttgag cacgtagacc cgcttcgtcg
     1261 agtcggacgc cgcgatggcg atcggcccga cctgcaacgg cagcttgaac tcgggcacga
     1321 gctggccgcc gcgcgggtcg atcatccgca gcaggtagtc gtccgacgag gtggccacga
     1381 cgtgatcgcc caccggcagc aggcgcaccg ccccggtgcc ggtctgcagt tggaccggcg
     1441 cgccggtgac cgcggcgccg ttcccgaact cgttgccgac gaacgaacgg ccgttctgcg
     1501 agacgacggt gtagatcaag gccgccccgc ccacatcgat gagcgcgacg tcgtcgttgc
     1561 ccgacaccgg cagggcgatc tgccacccct ccggcggcgg gttgggcagc gtgaactgga
     1621 acaccgagct gtatgacgtg accggctgtc ccggcgcggc ggacgtcgcc acggcccggc
     1681 cgtcgggcag caccgagagg tggccgaccg ggttgaacga ggccgggaac ggcatcggcg
     1741 acatggtcgg atccaccgag tccgggttga tctggtacag cccggctccc tgccactgga
     1801 ccttgttgtt gaccgtcacc ttcttcaggc cgatggcgta gaccgcacca gggatggctg
     1861 cggccgtcgc cagggtcacg aacttcacgc cgcagatcgt cacgctcgga cgccagtcga
     1921 tgccgttggg acggatgtcg ccgaccctga agaacgtgtt gtcctcgttg ctcgccggca
     1981 ccgccacata gatctgccga ccatcgggcg agaaggcgac gtcggtgacg ggtccgtagc
     2041 cgctgaccgg cttctcccct gccgccagct ccttccctgc gaccgggtcg atgcgcgccg
     2101 tcatcgtccc gctcgtcagg tcgtaccgat tgatgaccgt cgtcggctgc agtgggttcg
     2161 tgcccggtcc gaccgtgtag gcctcggccg cgccaggccg gacccgcagc cgcgcgtgcg
     2221 aacccgcgcc gaagatcacg tccatgccgc tccacgtcgt gggataggcc gcgggcgtgc
     2281 gggcgttggc gaacatcgcg cagaacgtgt cgctctgcag gcgctgcctg aggtcgacgt
     2341 agaactgctg taggagcttg tcttccgacg gggagatgaa caggttctgc cccgacgtct
     2401 ggttgaccgc ctgcgcggcg acgttggtga gcgccgagag cagttgccgg gccgggctcg
     2461 tcaccccgtc gtccggcccc ggtgtgagct tctcgtggat gaagtcgtgg aggctctgga
     2521 tgttgtcgcg gtagaagtcg ctccagagcg tgccttgcag cagcgccggc ggctcgtcct
     2581 gcgtcttgtc gtacttctcg accgcgaacc cgatctggag ctcagggtcg ctcgtcagca
     2641 gcaggcagac gtcgccgttg ccgtcgccgt ccaccgcgtc ggggaccgcc gcagccaggt
     2701 cgagcagatc gaccggcgtg ctcgcggtga cgtcgaggtc gttgccctcg gcgtcgatcg
     2761 ccgtcccggg ctgcacgatc acctggcttg gcgcggcgca gtgcacctgt aggccgttga
     2821 cgatgcccca gccgtgcagc aggcggtggt gccggcggag gtcgtgctcc ttgcagagct
     2881 gatcgagcgc ctcctgcacc gtgtccgcac cggggatcac gcacgccgtg ccggtgtagg
     2941 cgacatcgga agctgcgagc gtggtgagcg acggaaacag atcgcggcag tcgatgaccg
     3001 tggccggcgc gttgtccgga acggagacga gcgcgacccg accccacgcg tgggcgacgc
     3061 catcgggccg gcgggccagc gggttcccgg ccgagtcgcg tgcccagctc acgccgccgg
     3121 tcaccgtgcg agccggcacg agccagtagt cgccccgccg gtaggtgccg ccggacggga
     3181 agagcacctc gaccccgtcc tccagcgcga tccacgtgtc gaagtccacg gtgaccgcgc
     3241 cgggcgagtc ccaacggcgg gccttcggcg tgccatggaa gtccgtgaac gtcgtcgagc
     3301 cggtgggtgc gtcgggccct gacgtggcaa aggtgagcgt cgtccccttc gcgtcgagca
     3361 ggttgacgag ggtcccgggc gcgccggaca gctcgtggac gtcgtcggtg agctcgaccc
     3421 actggctggc gccgaacccg agcacggagt ccttcccgat gtcggcgacg gtcagctcgc
     3481 ccgcgcctgc gccgttgccg agccacgctg tgacgaccga accgttgtcg cggctccaca
     3541 gcacgatcgg ccgtccgtca tcggtcacgt cgtggacctg cacccggtag agctggttct
     3601 ccagcccggc gaagccgccg gccgccgtcg gcgtgcacag gttctgggtc gtctggtcga
     3661 gctcggcccg cgccgccatc cgcccgctcg gctcggccag cgtgggggtc cactgcgaca
     3721 ggtcgctgcc acacgtcagg ccggccggcg cgccctcgag cgtgagcagc ttgagctgcc
     3781 agaccgtctg gctgcgcgtc gtcgtgtccg gtccgccgag ggccacctcg cggaggtccg
     3841 gctcctcgag ggcggtcacg gcgcgctgcc agacatccag gtaggccaag tacatccctg
     3901 ccggcagcgt gggagtgggt ggcgtgttgg cgtcggcctg ggtcacgaac ggggcctggg
     3961 gcaggtccgg ctgcgtggcg tacgagcgcc ggcgagacag ctttaggtgc tcaccgagcg
     4021 cgttcgtgtc cggggtcccg tccacggtca cgactgactg cgcggcctcc accgcaatga
     4081 cccgcagcgt caccgccgag gcgtcgccac gaaggtccac ccagtcgtgc ggcgcgagct
     4141 ccgcgccgtc gagcacgatc gccgcgacag ttagggcgct gccggcgacg gtcgttacga
     4201 cagtcgtgtc cgcttccagc tcgcacaggg tgccgcccac ccacgcccgg cccggcgaca
     4261 gaagcaagtc ggtaccgtcg ggcgcgaggg acagctcgaa cccggctgtc gccttcggga
     4321 cgcccgtggg cccgatgaca tcgagggcct cggtctgctc gaggtggcgc tcgatgtcga
     4381 cgagctcgtt ccagtccgcg tcgagctgca cccgtccctg ctgcatccgg acaccggtgt
     4441 agtgacgggt cgcgacgaac gtgctgcggg agatgtcgcc accgttcatg tctgctccct
     4501 ctcaggtcgc atggatcagg cccgcggtga ggccgaagcg gaggtactcg tcgagcgcga
     4561 cgccgacgtt ggcctcgcgc tgggcgcgtt gcagcgaagc gaacgcgccc atgtcgctct
     4621 gggtcgacga cccggcggtg agctcgacgg cgcaacggtc ggcgagctgg ccgaaggcag
     4681 gatcgcccaa tcggtccgaa gcgaagaccg ggcgcacccg agcccgcacg gcaaccgggt
     4741 cgaccgtgcc aacggacggg ttcaacgcca ggtcaggttg gcagcggtag cgcctcggcg
     4801 tcgccgagcc gtcacccacg tagctgaagc gcacgcaccc ggcctgcttg cgctcgacga
     4861 cggcggcgcc ggtgacgatg gcgtcgctgg ccaccagact gcgcgccgcg agatcaccga
     4921 gcacggtgac ctggtcgagg gtgacgtcca cctcagcggc gccgacatcc ccgtcgacca
     4981 ccgcctggga gaccgacaac gtcgggcccg agcctgccat cgtcaccgga ccgacgacgg
     5041 cttcgccgac cacgaccgaa agatcgtcgt cgccggccac ttggacgcct gccgtcggga
     5101 gggtgcagtg gagcacgtgc agctccccga ggtctccgct cgccacctcg accgcgcctt
     5161 cgacgaggag accatcgagc agcatccggc cgcgcgtcac cgagtcatcg ggcggtgcgc
     5221 ccccggtgat ggtgacggac ccgaggatgt gcggccgagt gacgccgagc gcgatctgcg
     5281 ccggctcggc gacgtcgccc ggctgcgggt cggcgacctg gggccagctc gcggcggcca
     5341 cgagaagctc gctgccagcg gggatcgtga gggccagatc gccggcgtag gtgcggctgt
     5401 ccaggagcgc gatcacgccg acggtccctg ccggctgggc gttccagtcg tccactgcct
     5461 cgctcgcggt cgtgacgacg ctcggtccgg cgggcaggtc cgtcccgacc gcacgccacc
     5521 acgaggcccg ggcgagcagg tcggaggtca ccgcgccgct gcggtcgtag gcgccggcgc
     5581 cgatcggccc ggcggcgaga tacgtgtagt cgacctccac gagcgacggc acgacgccat
     5641 cgcggaacgc cagccggccg aggacagggt cgatcgccgc ggtcaacggc gatcccgggc
     5701 ggcgccagcc agtcgtcacc gaaggcggcg ggtcggacag gtccgccgcg gtgagctcgg
     5761 cgacgggcac ttcagccagg ccggcgccgg tgtcggcgta gacccgcacg acagggtcgg
     5821 cgctgaagaa caccggcgcc tccaccgtgc cggcccgcag tccttcgagc tcgtcgaaga
     5881 ggaggcgtcg ttcgagcgtt cccgggacgt gctgctcggt ggccagggag gtgatcgtgc
     5941 tctcgggctg tggcgggttg gcgagcggca cgtcgagccc gagcgggtcg aagtggtagc
     6001 ggccatccgg tgggtcgctc accgggcgtg ccgtcgcccg ctcgacgcgg aacggctgca
     6061 ggcgccacac cgccagcccg atgtcgggca cgttgctgat gcccgctggc ggcagccgca
     6121 cctcggcgag gcgggcggag gggtcgaagg cggtcccgat gcgttccaac ccggccgcgt
     6181 cgtgcaggtt gatgccctgc tggttctgcg gtcggagatg gttgacgttc tgtgtcgtgc
     6241 cgacgacggt gaacccctcg atgacgtggg cgggccagcc ggtcacgtct cgggccagct
     6301 gctcgagcac ggccgcggtg cccttgcggc gccggtaggc caacgtgttg gcgacatagg
     6361 ccctcggcag ctgcgtccgc ggcccgacct ggtggaggac ccgcacaccg agcaggtcgc
     6421 cgatgtagtc gacgacccac ggatcgcagg tctcgatgaa ccagttgtcg tacagctggg
     6481 cgatgtcgtc ggcgacgatc tgggcctgct cgccgaggat ctcgaccagg gcgcgcagtg
     6541 tgcccgattg gggcgattgg gcgtcgcgct ggcggtagac cgccggcacc agcgcccaga
     6601 gctcgtcgac caaggtcacg tcgaggccac cacggtgatg ccgtcgccgg cgacggtcag
     6661 cagctgcgcc ggggcgaccg tcttcccgtc accccgcgct ccccgggcgg cgaggatcga
     6721 ttggcggcca ccgccgggcg tcgtgtcgag cagggtcagg ttcaccgcaa cgacgcccgg
     6781 cacaccttgg gccgccgcca tgacctcggt cgccgtcact ggttgaccga ggtcgcggcc
     6841 atcgaacccg aactgcgcca acaacgcggc gcggaccgcg tcgatgactg caccgggggc
     6901 gcggtccggt gcgaccacga ccgtcagggc gacgtagaag cggatcaact cggcgctgtc
     6961 gatggcgacc agcagccgct ggtcgccgtt gctctggatc gtcgccagca ggtcggcgcg
     7021 atcggcgtcg gtgaccgcgc cgccgccatc agcggcgacc gtgaggtgca ccgcgtacgt
     7081 gccgttgacg aggagctcgg ccgcctgggc tttggcgtag ccaggaatgg ctcgaacgaa
     7141 gtcctcgaag tcgagcaggg agacgacccg gtcgaacgcc aacgcagtgc ggggagcgtt
     7201 gcgtcgcacg agctcgggtg ggtccggatc agcagcgagg gtcggcgcct gcgggttgct
     7261 gacgcctcgg acgccgagcg gacgggtcat cagcatgctg agctggccgg cgtcgaccag
     7321 ccccgccgac ccggtaccca cccggtacac cgcgctgacg ttctgcgtcc cggtggggag
     7381 gcgggccccc gtgatgccgt cgccgaagcc gacggtgacc ttgtcgtcgt cggcgatgcg
     7441 caccgtgtac acgcggtcct tcggactgga tccgaagagg gaccccacct cacgccaccg
     7501 cgtcccgtcg atccagatct ggagggtgct cgtcaccccg gaggtcgtgc tcgacacgta
     7561 ggtcaggggc gcttgggcca gcgtgaacga ctggaacgtc gcgctggcat cgccgttacc
     7621 gaggatctcg cgcttggtct cgccgtgtgt ggccggcgcg acgttggccg cgaaccgcac
     7681 cgtgctgcga tcgaacgacc catgcagtga ttgttgcaac tgcaatgtca atccgtcctg
     7741 atgatcgacg taggccagct cgaccaccgg cgcggatccg gtggcaccgg agaggaacgt
     7801 ggccgaaccc ggcgacacgg caacggtgcc ggtgtcggcg ccgtgctgga ccggccagac
     7861 ggagctggac ccgacgctcg tggccgggcc ggtgatcgtc agctcgtcgc cgacctccag
     7921 gttctgctga ccgcccgatg aggtcgtcaa cacgagtgaa gcggactcga cccgcatgat
     7981 cggcgactgg ccgacggcca gcacggctcg cccggcgggc aggttcgtga ctgggccgcc
     8041 gagctggatc accttgccct cgaccggtgc ggccagcggc gcgccgacca gcgacagttg
     8101 ctcgctgccc gccaagacgc tgacctcgcg cggtgtcgat ccggcgtagg ccgacgacat
     8161 gctggtgtgc agcgtgaggg cagtgacccg ggccgacaac gcgaagtccg agcgggacgt
     8221 gtcctggacg ccatcgatca cgaacaccgc cgactggccg gctttcgtca gcaccacgag
     8281 cccaccgggc acgagcgccg gataggtggt gtcgagatcg accgtcttgg gatcctcgga
     8341 gacgacgaag tccggccact cggtcggcgg ccaggtgaag aactgcagcc cgccctgctg
     8401 catgcccgtc accttgtccc cggtgttccc gatgaccttc tgggccacgc gttctcctcc
     8461 tactcctcga atcgacctag atgaagatgg gcttgtagtg atctcggatc gagtccggca
     8521 gcgtcctcca atcgggtgcc gacgcgccga acaccgccgc tcgctgatgc agcacgtgga
     8581 cctcgtcgta cgacgccggc acggctacgc cccagaccac ctcggtgatg ccggtcggca
     8641 gcggcgtgac gcgctggatg gtgtggagca cgaacgctcc tccaggccct cggaacagca
     8701 aggtctcgcc ggcgcggatc gaggttgccg caccctcgac gtagaggcgg ccgaagggga
     8761 agccgtcgag cggggagccg gcctgcgcca cgccgatcgc gttccactcc ggtcgagcgc
     8821 cgacgtcggc gacggtctcg aacgtctgcg gcgtctggcc gggaccggga aggctggcga
     8881 ccttcgtccc ggtcgggatc ggcacttcgg cgggtgcccc gacaccctct tccaaggtga
     8941 actggagcca cgtcgctgcg gcgcgaccgg gcgatcgctc gtagcccacg gcgcgggcca
     9001 gcgcggtgac cgacagaccc tcggtcgccg tgcgcagata gccctcggtg acgatgcgct
     9061 cctcgtagaa cgcgagcacg tcgagcacgc aggcccacgc atccatgagc gcgatcgtcg
     9121 gatcgtccgt gccgcgactg cgcagagccg gcagcgtgct cgacagccgc gcccgcatcg
     9181 actcgaggaa ggaaccggcc gtgcccaccc ggtagtcgag ccggcgggcg ccaggtgggt
     9241 tgtcggtgct gcgtggcgtc gccgcgtgga cgccgtcgca gcaactgcag tcgctcagcg
     9301 gcgcgacgtt cgtggcgtcg acgtcactca tgggcgggtt ccgatcgaac gggtcacagc
     9361 ccaccgacca cgttgagcgt cagacgaccc cgctcgggga agttcgggtc gtcggcgagc
     9421 tggatgatct ccagcccgtg ggggacgagc acaccgtcgg ccagttcgtg ccctggggtc
     9481 ttgccgaacc ggtgcagcgc cgtcgcgttc acctgcgcca cgcccggcac cgtcagcacc
     9541 gcggcgtaga cctggctgag gtacaggggc tggccgaagg tgaagttgtc cggatggaag
     9601 aagccgcggc ggccgtccgg cagcacgcgg ctgctgagca cgtccagcac ctggctctcg
     9661 acctgcgtgg cgaagtagcc gggtcgcacg cagatgtcca gcgcgagctc gagcggcagc
     9721 ggcacgggct ggttcagctc gaagtcgacg cccgccatgc gttgggtctg gatgacgtcg
     9781 gagacgctgg cgacgaggtc gggcgccagc gcgccaccac cgatctggtc aacggtgatc
     9841 gcggccgtgt accacgaccc ggtccagcgc aggttcgtgg ccgcccgttg cacacccggc
     9901 acctcggcgg tcacggtcga gtagtcggcc gcggtcaccg ctcgctcctg cacccgatag
     9961 gcctgcggcg ccaggcggcg gacctgctcc atgggctccg ggtcgacacc accttgcgcg
    10021 ggcagcgggt tgcgcacctg gtcgacgccg ctctcggctt cgcggagccg gtcgagcgcc
    10081 tcgccgggga tgttgccggg ggtgccgttg ccggtgcggt aggtagcagt gagctccgcc
    10141 ccgcgagccg gctccatccc cgacaccccg tcgccgaagc ggatgcgcgt ctcgccgccg
    10201 tcctcgatct cgaggacgaa gacaggtgcc gtggccgctg cgtcgacgag gtcgccggcc
    10261 gggtcggggg tccactccag ctcgccgtcg tcgagcgtca cgacgggatg cgccgtccgc
    10321 ggatccatgg cgaggagcgc ggcggccggc gcgtgctggt catagtcgac gacctgggtc
    10381 acggggccct cgggcagccg tggccgccag cgtccagggg cgtcggtcgg atcgtccgcg
    10441 agtggcagcg tggcctggcc cgagagcccc tgatcggcca gcgcgacgtt cccccgcgcc
    10501 acgccgcaga ccacgtgctg atggacgcca tccaccgttg cgatgttcgt gacgcagagc
    10561 gggaaggcca gcgcgtcacg ggacgaccac gccacctcga gcaggctggt gctcgtgagc
    10621 gggtcgttgt cgggcgtgac gtcggtgagc cgcacgacct ggcggtgggt cgggtcggca
    10681 tcggcgggat cgccggtcgt cggtccagtg gactcctcga agaggaggaa gtcgccgacc
    10741 gcgagctccg gaccgtcggt ggcgtcgagc gtcgcccggg tggcgcccgc gggcaggcag
    10801 cagaggtcgt cgccccaggt gtagagcggg atcccgttgt tcgctgcccg cagttcgacc
    10861 tcgtgcagga gttggaagac cacggcgtcc tcgtccggac cggcttgtac ggctcggccg
    10921 gcgtgcagcg tgagtccggt cgaggccgtg aagtgcacga acgtccgggc gctgcagccg
    10981 tcgtgcatcc ggtagtcgag gagccgggcg tggcggcgaa cggacacccg ccgccgcgcc
    11041 gtcgtcaagt aggcctcggt ggccaccgcg tcctggaagt aggagaggcg gtcggcgacg
    11101 taggcgagca gctcgacgag catgagctgc tggtcggcgg cattgcgctc ggtccagccc
    11161 ggaagggtcg tggccagccg gtcgagcatg agcccgcgga agctcgcgta gtccttggcg
    11221 aggtagtcga gggcgggctc gtcggcgagt accggcgggc acgacacgac cgcggcacag
    11281 tcgaggtcac tcgggcaacc cgccttgaag gagaaggtga cgtcggagag gcgcttgtcg
    11341 tagccggcag ccggctggtc ggggctgacc ggatcgacca ggcgcagcac atagggcgag
    11401 aagtcgccgg gccggtccac ggtgaccgtc aggacgtccg ccgccgtcgg cgtcgccgtc
    11461 agcacggtga tgcccgtggc gcgcacgccg ccctcgacac gcacgttggc ggcgttgagc
    11521 gccggtgacg cggcgaggtc gcgcaacacg tggacgtcga gcgcggtctg gtcgtccgac
    11581 acctcgagga agtcgatgcc gttcagggtc gtggactccc gaaccgcctc cgctcgtcgc
    11641 gggttccggc agcggaactg cacggtcggc gggttgacca cggcgctcac gcgaggctcc
    11701 tgacgaactg cgcggtcgtg tcctcgcccg tacggttgac gacgtactgc accgtcacga
    11761 cgagggtggc gtcgtcggcc accaccacca cgtccttgac ggtgatgagc gtgcccatcc
    11821 actgcgccag cgcgccctgg accgacgcct gggtggccgc ggcgagcggt tcggagttcg
    11881 gcaggaacag gagttggagc aggccggtgc cgaacgtcgg ccggttcacg cgctcccccg
    11941 gcgcggtgaa cagcagttgc tcgatcaggt cgcggacatg tccgtcttga tcggtgagcg
    12001 cggtggcgcc gcgccggtcg acgtggagcg gatagtcgac ctgcatcacg tgcccttcac
    12061 ccgcaactgc gtcacgagca cttggaggcc ggtgccggtc ggggtgcaca ccgcggtgct
    12121 gtcctggagg acgagcggca cgccacccga ggtgaccctc gtcgcggcca cgacccattg
    12181 cgcgctgacg caaggcggga tcggcggcgg caccagcgag caaccggcga ccacgtacgg
    12241 gccggtctgg ccgacgaccg gttggttgct gacgagcacc cgggggatgg gaacggtggc
    12301 ctgcgcctgg ccaccgtgca tgcacagcac ggtggcgccc acgtggagca ggtagcccgg
    12361 cactcagatc acgtccagcg cgccgttgtt gacggacacc ttgggaccct gcatcgagat
    12421 gctggcgccc ttgccgttgt cgatcgtgat gccgagcgag gtcatctcga acttcgcccc
    12481 gtccgccgtc tcgagcgtta tcccgcccac gccgggcgtg tcgtcgaggg tcaccgaatg
    12541 accggccggc gtgatgacca tgaccttctt gtacggcggc gcgagtagga gcggcggcac
    12601 gtcggcggcc gagccgtacc agcacccgga ccagatcggg tagtccggat cgccgtgctc
    12661 gaactcgatc cagacgcctg agcccttcgt cggcagggcg aagaaaccca cggacgaacc
    12721 ggcgaacggc agggacggca tggcccaacc ggactcgtcg gacccgagca cgtccgggac
    12781 ggtcgcccgg atgcggccga tcatcagggg gtcctcgatg tcggtcacga cacctcggta
    12841 cttgccgaga aacggtgaat tcatggcagc accgccggta agagcgagcc cgtgccttct
    12901 cgggagagtg tgaacgcctg gcgatacgag cccttgcgga tggaatgggt gacgctgcgc
    12961 acgaagtaga agccgtcgta gtcacggccg gcgccgcgca cgccgacgag acctcgggca
    13021 cgcagcaccg agccgtagcg ggccgtgtcg acctcgccgt tgcccgtgac cgcctcgggc
    13081 gcccgtgtcg ccgcggccgc ggacgccaat gccgcctgga tcgggctctc gttgcccgac
    13141 tctcgcagca gcgtggtgcg gctcgtcggt gtcggcgacg ctgcgagcgg cggcagtcgg
    13201 agcgccggca gcgccgggat ggggatcgcc accttggtga tcggctccaa gaacgagccg
    13261 ctcgcaccta ccggcgcgag gccgtcgttg ctgaaggaga gcgagttgca gttcgcgtcg
    13321 ggcccgaggc cgagcgtgat cgcgggctgc gggatcccgg cgcgcacgac cggcccccag
    13381 taggccgtgt tgacgccgag cgtcaacggc tgcacgtaga acacgaagct gttgcggtcg
    13441 gccgcccgtc ggatgaaggc caggtccgtc tcgtgctggc gcgggatccg gtccatctcg
    13501 atggggaagt cggtggtcgg cgtgaccatc gggacaagcc cgtactgcgg gtagctcagc
    13561 aggatctggc tgacgatgac gtagtcgggc tggttgctga agggcgcgtt cttctcctcg
    13621 aggtcgagct ccagcgacac gtcggtgccg gtcaccgtga acgtcgaccg acccgggtcg
    13681 tcgctcggtc gtaggtcgtg acgggtgatg acaccgtcga tgagcacttc gggaaccacg
    13741 ccgagcacca ccgcgatcca cacccggttc atcagttcga gcgcgccacc gtcgaggacg
    13801 tcgtagccgc cgaacgtgtc cttcccgacg ttgaacgtca tctggaagcc gtccccggcg
    13861 tccgtgtcgt tcgtgacctg tatcgactcg agcgcatcag ccagctcgaa gtgcggcgta
    13921 ggcaccagac cgccggtcca gatgagcacg cggacaccga gcaggctctc gagcacggtc
    13981 agccgatcct catcgcgatg gcgacgacgc ggccgacgac ctcgagatcc tccggccgca
    14041 gcacgtcgtt ggcgtcacag agccgccaga actgcaaggg gtcgcccgcg tagcgagccg
    14101 ccagcaggtc gagtcgctcg ccggccgcca tgcggtgctc gacgagagtg gtgtgcgagc
    14161 ccgccgcggg cacgaaccgg cgccgcacgt accggatgtc acgggtgtcg ccgtcgggat
    14221 cggacacggt caccgtcacc gtgtcgatcc cggcgtagcg gctggtcttg tcgaacacct
    14281 caacccccga gctggatgct cgccgcggcc gagaacgacc cgagcgacga gacgctgttc
    14341 acggtggcca tgacctcctt ggcgatctgc tgcgccatga agagcgcccc gcccgcgctc
    14401 agcaacccga ggtcgtcgta gttcaggacc cgcaggctga ggctcacctt ggcttggatg
    14461 gggttcaacg ccgggtcgta cgcctgctcg gtgatcgaga actcggtcag ccgcacgggc
    14521 aggacgcggc gaggtcccca cacgagcacg gcgagcggcg cgtccggtgc gatcacctcg
    14581 atgatcccca gcgcggcgag cacctcgttg gcgatgacga gcgccgactt cgggtagagc
    14641 atcagctcga gtgatgcgag cgccggtgcg atgccgagct ggaccgcggt ggagtcgccc
    14701 tgttcgagct ggtcgaccgc gtcgatctcg atgtcgaagg tgagcgtctc ttcgggcggc
    14761 cctttcagcc ggagctgctc ggcctgggag ccgcccgact ccgcggtccg cgccatcagc
    14821 cggcgggtca cctgctcggg gttgtactgg aacacgatca cgctcgccag cgggttgagc
    14881 aggtcgaggc cgatcaacgc gcctcgcgtg acgcgcggcg agccgctgaa gccggtcacc
    14941 gtggctccct tctccgcgac gttcccccac cttcgaccgc cgctcgcagg tcggccacga
    15001 ggcgctccgc cacccgcgga cccaggtcct ccggcagacc gccagcaacg agcaccccgg
    15061 ccattacgtc gctggcgaca cggtcgtcag cggcggacgg cagcaccacc gagtcgatgc
    15121 gcacgcggat cgtcgtgctc atgacggtgt ccccggccag ggctggtcga gcttgacgca
    15181 ctcgtcccgc gcagcttgga agaggtggtc catcgtcacc cgccggccgt tcgcggcggc
    15241 gaggaaggcc gcgttgatgg cgatgttcct gatgctgccg ccggtgaggc cgatgcgagc
    15301 gaggcgccgc gggtcgaagt cggcgagcgg ggcctccggg ccgaacaccc cgttccagat
    15361 ctgctcgcgg cggtcggcgt ccgggaacgg gaaggtgatg acgaagcgga tgcgccgttg
    15421 gaaagccgga tcgagcgcct cctgcatgtt ggtggtgagg atggcgagcc cgtggtactg
    15481 ctccatgcgc tggagcaggt agctgatctc gatgttggca aagcgatcgt ggctgtcctt
    15541 gacctcggtc cgccggccga acagcgcgtc ggcttcgtcg aacagcagca ccgcgctcgc
    15601 ctcgtcggcg gcgtcgaaga cgcgccgcag gttcttctcc gtctcgccga tgtacttgct
    15661 gacgacggcg gacagatcga tccggtacag gtcgagtccg agctcgcccg ccaccacttc
    15721 ggcggccaac gtcttgccgg tcccgctcgg cccggcgaac agcgccgtcg tgcccgtgcc
    15781 ccgggcgctg gcgccgcgca tgccccacgc ctcgaagacc gtcttgcggt gacgcacctg
    15841 gttggcgatc cgatggagcg cgtcgagctg cgccgccggc aacacgatct gggcccagcc
    15901 agcgccggtg tcgacgcgct gcgcgagcgg gcccagccgt ggacgcgtcg acgcccgaca
    15961 gcgcgcccac agctcgcccg gtcgggcagt gcgtgccagc gtctggatga cctgcgggtc
    16021 gaggtcgaac tgctcgctga ccgcggtggt ctcctggtcc tcccctcgca gttcccgcca
    16081 cagcgacgcc tgctcggttg ccgtggcccg cttcacgtcg aggccgagtg cgggccgggc
    16141 gtgcaggtcg atcgggtcac gagcacacag cacgacgaga ccttcggccc ggtcgaggaa
    16201 caggcccgtc gatgccgtct gctcgatatc gtcggcatcg atgcacagga tgcggccggc
    16261 gaggacggcc tcccggtcgg tcagggtggc cagccgggcg aggtcctgcg ggtcgctcgg
    16321 caggcctcgc gcatcgagga cggccaggcg ggcaccgagc tgcgcggccg cgtcggcggc
    16381 gactgcccgc ttggcggccg tgccccgacc gagcagctgg ggaacgacct cgggcgcggc
    16441 ccgccacgtg tcgacgagct gcgccgcaac ctggacctgc gagtccacga ggtgctcggg
    16501 gaccggctcg gcatggacca agccgcccag gacctgatcg gcgtcgccga caccggcgag
    16561 gacgtggagg acacgctcgt cgatgcgcag ctcgcccgtc accggcgagc tgccgtgcag
    16621 ctccaccagg caccaccgcc gcaacggccg tcccgggctg agcgccgacc agtgcgcacc
    16681 gggcagcgcg gccagcgcca acccgaaggt gggatgacct tgccggccgg ccgacacctt
    16741 cgagacaagg tcggcgaacg acgagtcgag ctcgaccccc gcgcacagga tcaggacgag
    16801 gcgttcgaag gcagacagcc cgaaccccgt gcagacgacg tcgagcgcgc cgggcggctc
    16861 cgtgtccgtc gtggacgccg cggtcgtcgt ggtcgaagtg gtcgaagttg catcagcaac
    16921 atccgtgggc cggctgtcga cttcagcatc ggcagcgctc gccagctgac gttcgagcat
    16981 ggcgcggacc tcggcgacgg ccgcgctcag gtggcgctgg ttcagctcgg tccactcgtc
    17041 gtgggtcacc gcggtcatgg cagcgtcacc gtcgggttgg tgatgtggcc ggcgccgtcg
    17101 cgggtgaccg ggcccggcgc cccgtcgacg agcagcacca cgccgtacgt accgccagcg
    17161 acgccagcta ccgagacacc cagcgaggtg ccgtcgtcgg tccggtcggg cacgttcaac
    17221 agcgcgacga ccgcgtccgt cgcggtgttg accagctcga ccgcggcacg ctgccgggtg
    17281 ctcaccgtga cgtccgaggt caccatcagg gtgccgccgg cgtgggcgac gctgacgacg
    17341 cgaggatgga gctgcactgg gtagaccggc gacgagacct ggccgcgcgg ctcgggcgga
    17401 tcgccgacca gccgggcttg gacgacctgg accgccaccg tcccggcccg cagagcaccg
    17461 gctggcgccg acgcgaggtc aaccaccacc gaagtcgcgc tcgtgctcgt cggcgtcagg
    17521 tccacgccgc cgaggcgcgc ggtggtctcg tcacccttca gctgcgtgcc cgagatctcg
    17581 atcacccggt tggcggtgat cggcgcgaac ggcgacccgg aaacggtgac cgactcgatc
    17641 gtcggcttcg ggatcgggaa caccgacaag accggactga ggaccggcgg tcccgtcgag
    17701 accttcgacg cgtcctcgat cagcacgacc gacgcggtgt acgcggtgga cagggcgtac
    17761 ggcgtctgga agaagaccga ccacagcttc gacaactgct cgaggtcgag gggcatcggg
    17821 cagacccgca cgacgtcgat ggcgtcggcg agatcggtgg tgacgaggct gggatggatg
    17881 gccgggttgg cgttggccgc ggcgacgacg tcaccgatga gctcgcgcgt gagcaccggc
    17941 tgggtgtgga gcgtgcggac cgcgctgccg aggaggcgct gcggctccaa ctccttgtcg
    18001 tctccgtaga acgtgaggag atagtggagg tcgagcgcgg cctgggccgg ttgcacctgg
    18061 gtgccatcgg agcgccgggt cggcaggtcg acggctcgag cgaaggggtc ggggctgacg
    18121 tggtagaggt agacgttgat ctgcggcgcg gtctggttct tcttggtgtc gggccggtcg
    18181 gtgctcaccg tcgcgccagg cacgtcgtcg gcggctggtt gcccgagcaa ctgctggatc
    18241 gccgcggtga ccgtggcaac cgccagaaag ttgctcactg gtgcacaccc cgcgcatcgc
    18301 cggccattcg accgctcacc acgggggaag caccagtgag cagtcgcccg cgtcgcggcg
    18361 gagaactcac cgtcacacgg agctcgcttc gctcactcat cggcccgccc gccgttgctt
    18421 gaggtagtcg gacaggtcga gcggcggccg cgccggtggc cggcggtctg ggcgggctgg
    18481 tgcaattggg tgcggtgccg ggatctcgac ccggccgatg ctcacctcta tcaggacgtc
    18541 ttgaccaccc tcagtgacgc ctgaggttcc cgatcgtcgg tcgaccgtgg ggcccgacgc
    18601 cctggccgtt ggcgtggtct cacggcgcct cgccgtcgga gccgttgtac gagccctcgc
    18661 cggctgcggc gcgtccgatc ggcgggtcgg cgtgctctgc ggcaccgctg ccgcggaccg
    18721 ctcgtcgcgg tcacgggtcg ccacccgctc gcgctggtct gtcgggggtt cccgtcggac
    18781 cacggcagca gcgtcagtcg gcggccgacc gctcgaccgc tgtcctggac gtgccggcac
    18841 ctcggccgcc gcgccgcgtc cggcgtccgc gccgggcgca accgccggca cgtccccggg
    18901 gatgcgtgcg tcgactccgc cgtgagtgtc cagggcggcg agtcgcggcg ttgtggccgc
    18961 gggagacgcc ggcgcaggag tcagtggatg ttccccaggc gcggcgacgt cggctgcagc
    19021 ctcaacgttc ccctcgagca gcgaggcggg tgcgaaccgc gacggcggcc ggggtcggag
    19081 cccggtcgcg tcggcacggg cccggccgac gaggcgctcg agatacccgc tcaccggttc
    19141 aaggcctcga ggtacagctc acggcgccgg ccgctcatgg ccagcacctc tcgctcgctc
    19201 cagccgtagg cgcgggcgag cacgccgacg tcctcgctca ccgagcgagc cgcaccgacc
    19261 gtcgccgacc agaagaaccg caccggatcg agcacctcga tccactcctc gccacaggcg
    19321 gcacacgtca gcgcgacttc gagctcggcg cccggatcgc tgagcgccat cagggcctcg
    19381 atctcgtctc ggacccccgc ggtcgccgtt gcgttgacgc aacgctcgaa gagctcgtcg
    19441 gcgtctcgag ccgcggccac atcggccgcg gtcggaaggc ggaacgagag cacatcgtcc
    19501 gcgatctcga tcgatcgcgc gccggccgtc ggcgggtcga ccgcgaggtg tgcggtcggc
    19561 acatccacat cgagcgactc gccgcacgcc ggacagtcgg tgacgccggc cagcgacccg
    19621 ccgtacgtcg cggcgtgcag ggcgagcagt gcggcgtcgc gctcccccac agtccgttcg
    19681 cccgcgtcga tgccggccaa cgacagcgcc atgagggcgc gctcggccgg gaccagcctc
    19741 cctccgtggt cccacaggtc cagcaccgcg gcgggtccga gcgcgtccat caggtcggcg
    19801 ggttcgtgaa cgtcggttcg gtgggctcgg gcacctcctg gtcccgctcc caaccctcgt
    19861 tctcgagctt caggtgctgg atcgccaccg cgttggcatt cgcatcgaga tcaggcaacg
    19921 cctggtactc ggatacccag cagcggaaga tcttgtacgc gatggcgagc tggccggcct
    19981 cgttgtagac ctcgaggatc acgtccttcc ggaagtcctt gagcgacgtc tccgagccga
    20041 gcccggcacc aaagttccag accttgttgg cccacttctc gaactcggtg tcgtgggtga
    20101 cgccacgctc gagcgtgatg gcgtcgaact cggtgcgccc gaccgacttg cggctggtgc
    20161 tcgggtcgcc accgtcgcgg tgcttcacga cctcggtggt ccgcttgagc gcgccgacct
    20221 tgctgacgcc ggccacgtaa cgaccgtccc acttcacccg gaacttgaag ttcttgtacg
    20281 ggtcgaagcg agtcgggttg acggtgaact gtgccatcgt gcgactcctt tacgactggc
    20341 tctggccggc gagctgctgg atgcggatca ccacgaactc ggccgggtac agcggggcga
    20401 agcccacgag gatgttgacg acgccgcggt cgatgtcgcc ttgcgtcgtg gtggtgctgt
    20461 cgcactggac gaggtaggcg tccttgggtg tggtgccctg gaacgctccc tgacggaaca
    20521 ggttctgcat gaacgacccg acgttcagcc tgatctgcgc ccagagcggc tcgtcgttgg
    20581 gctcgaagac cacccagttc agaccgtcga cgaggctgtt ctcgatgtag agcgccatgc
    20641 gccggatcgg gacgtacttc cactcgctcg cccgctgatc cgcgccgacg agcgtgcgag
    20701 ctccccacgc cacgttgccg atcaccggga gcgtgcgcag cgcgttgatg ccgagcgggt
    20761 tcaggaccgc gctgtcggag tcggccagcg cgctcaccag cgtcgcaccg gcgagggtcg
    20821 cgtcgatgcc cgccggcgcc ttccaggttc cgcgcgatgc gtccgtgcgg gcgtagacgc
    20881 cggccatcat cccggacggc cccagctcac gcggccgccc cccggccaac ggatcgttga
    20941 tcgagatccg cgggtagtac accgcgccgt tcggcgaggc agccggtgct tcactgccga
    21001 gcagccacgt gcgcatcttg tcggtggtgc cgacctccgt ggacggtggt gggtccaaga
    21061 tcgcgaacgc ccgccgagtg acggcgtagg ccacgactgc gtccatcagg ctcttgcgcg
    21121 tcccgtggtc gacgcgtgac gtcgccggca cgcagagcag gttgatgtgg aacgggtcga
    21181 cgtgatcgag cggggagctg cctggcgtca ccgccgccgt cagggcgctg acgaagtcgt
    21241 cggtggcctc caggctggtg ccgtcgatga cgtccggcaa cgtggtcggc gtggcgagtg
    21301 ctcgccaggc tgtgtcgagg gggtcgccgc tgctcgtcgg cgtcgacagg ttcgggatcg
    21361 ccgcgctgcc gccaccgggc gcggagaggc ggatcaacgc cgagccggcg ttgacgaccg
    21421 ccacggcgtc gttggcgctg cccggggtca cgccgagacc gcggtagacc tccgaggcga
    21481 cgatgcggct gtccgaaccg gtggtgacca cctggcgcac gaacaggttg aacgagtcag
    21541 gttcggcggg caccgtctcg gtcgccgcca cctcgacttg cgacccccag gcgccggggc
    21601 tggccgcctg cacgacgagg gcgccggtgc cgggagagcc ctgtacgagc tcgatcgtgg
    21661 ccagcatgtc ctcgtccggt gcggccggat cgaagccggg gacgacgcgg acgatccacg
    21721 cgacctgacc gccgttcagg aagtacgacg ccacggcgta gctggcctcg ctcgcctggt
    21781 cgaccaggcc gaacacagtg gtgaactggg ccaggctcgt gacgcgtgtg gccacgccgg
    21841 ctggaccttg cgcgaagtag tcgacgaagg ccgtgtccga cgtgctggcg ccgacgatgg
    21901 gatggacccc gctcggcact tcctccacgt agatccccgg ataggtgtac gtcgcgggca
    21961 tgagcgcctc ctagtggagc gtttcgacgg gatccgaacg ggccgtgcgt tgacgagcgg
    22021 gacaggtggt caggtgtcgc atccgggtga tgaggtccct cgggtcgacg ggactccacc
    22081 ggtcgtcgcc gacagggcgc gacgaggtgt tgccggcgtg cagcgccgcg acgtaccaac
    22141 cctcgtccgg caaggcgagg aggtggtccg gcgcgagtcc ggccaggaag gcaccgtcct
    22201 cgcccacggt gacatcccgg aacggatggc cttcccacgc ctcgcgccgg tacacgaggg
    22261 tgccgccagc gagccacggc gggtcgtgcg gaccgggcgt gtaccgccac gcccgcgccc
    22321 gaccgcgatc gaggtaggtc agcgtcccaa ggccgcagac ctgcgcggtc gatgcgtcga
    22381 gcgcaccgac ctggtgacgc agacggctcg gcgccatcca gtcgtcgtca tcccagtgtg
    22441 cgatgtacgc gccgcgcgcc gcggtgcacg cgaggttgcg ggccgcgccg aggctgagcc
    22501 ctggtgtcgc gcgctggtag cgaacgctcg ggtcgcgggg cagcaggtcg acgatcggtt
    22561 cgatgcccga gtcgacgacc accagctctc ggaacgcgta ctcctgccgc cgccaatagc
    22621 gaagcgagcg ccgcacgaat cggcggcggt cgcaggtcgg catgatgcag ctgacgaggg
    22681 gcgcccgagc cacgaattgg gtgatacccc ggcggctgtc accagcgcgt cacgcaatcg
    22741 agctcgacca gctccgctct tggtgcagtc gttgatgcgt ggcacaggcc ggcaaggtgg
    22801 ccaggagcaa tggcgaagga tcgagtccga gctcctcgcg caacaagcgc cggcagtagt
    22861 cggcctggcg cagcgcctcg acgacgttcc cctccagcag gtgcacgtcg atcaccgttc
    22921 gatgcgcgct ctcacgcagc ggctcggcgc gcacggcggc gagcgcggct tcgagaccca
    22981 acgcgaagca gcccttgcgg gccaacgtca gcgccagcgc ctcaagcgcg tgcagtcgga
    23041 gctggcggat ccgctgccgc tcgatgagga cccagtcgtc gtaccagccg ggaagcagct
    23101 ccccgtcgag gaggtgcgcg ggcaaggtgc cgtccaggtc cggcacgcca ccacggatcg
    23161 agatcgttga gtcgtcaatg acgtcggtga cgtcgacccg aacgcgatcc gacagcgcga
    23221 cgcaggagtc cgtcgcgtcc agcaacccaa gtcgcgtctt gcgcatccgc cacaggagcg
    23281 agcgcagcga tccgtgggcg tgctcttcgc tcgcgtccgg ccacaacgcg ccgacgaggt
    23341 gggcgcggtc ggaacgacca cggagcgaga ggaaggcgat cagcttctgt gccccgaggg
    23401 gaaggtgctg ggctcttcca cccaccgtga gctgccacgt cccgagcaac gagaggcgcg
    23461 gatcggccac tttcgtgggc gcctcgcgcg gcccagacga gtagacgctc cggtccattg
    23521 ccctctcgat cagcaaggag cccacggccc tcaactctcc aaaccgactc tctggccatc
    23581 actcggttct tgtcaacccc agtcgggagg cggcatcgat gaacccgagc caccctcgac
    23641 ttggttgctg tcgcatccac ttcggtcaaa gttggttgcc cattcacgaa cgatgtcgag
    23701 caactgcgtg aggttgcggg ccacgctcga gacctcgggc aggcgctcga cgtcggcagt
    23761 cctggtcaca cgcgccctca tccccggctg accacaggcc tctcgccaca tccggatgat
    23821 gaggaccccg gtgcgttctg gagcgtcagc ccccacggtc gcagcatttc tcactcaccg
    23881 tcaccatgcc gtcacctccc ctcgcgctgg ctgcctcgtg atccaacgcc ggccagcgac
    23941 tggtagaagg cgcggtccgg cgcgggcagg ggcggttcgt cgatcgccat ccagccatga
    24001 gggtccacgg actgcccgca ctcgaggtgc cagctgttgg cacgatggcg gacgtagacg
    24061 tagacgccgg tcgcgtccag cgcctccaag cgcgccccag cgcggactgc ctgccgcacg
    24121 aacgcggcgt cttcggcgag cgagtcgtcc ggatagcgcg cgctgtgctc ccagactgaa
    24181 cggtggaacg ccagcgttcc ccccaggacg tccaaggcca gcatgtggcg gtgcaggtcc
    24241 gagctccaac gccaggtccg ccaggccgcc acgtcgagga gcggcacatc tcgcagggcc
    24301 gtgatgtccg cttgaccagc gaggatcggc gcgacctgcc tggagacccg actcgggccg
    24361 tgccagtcgt cgtcgtccca gaggatcatg atctcgcccc tggcacgtgc gcaggcgtcg
    24421 ttgcgcatga caccgatgct gcggaccgtt ccactcgaaa ccagccggat cctcgggtca
    24481 tcgggcaggg acgccgccag cacccggtct ccgtcttcca cgacgaccag ctcgaggttc
    24541 cgatagtcct gctgttggaa gtagtgcatg gcctgcaacg cgaagtcggc gcggccgcga
    24601 gtcggcatga cgcaactcac gaggggtgcg ccggtcgacg gcgccggcac agcccgcaca
    24661 ggtggggcaa ctgcggtccg cggcccagtg ggtgttcgac ggcgttcgcg cgagtcgcgc
    24721 cgccgggcgt gcgggttcac gaggtgtggc ggcgcgaagt gctcgcacgc gtcaccacga
    24781 cagtgcggct cgtagctctc ggtgcgtccg gcgaggcgca ggccgcagaa ctggtcctgg
    24841 tagcggtgcc ggctggcctc gccgtgagcg gtctcgtgcg ccgtacgtcg ggccgtgggc
    24901 gggctgcggt ggacgtaggc accgcacacc cagacaccgg tgaatccggc gcgcgccgcc
    24961 cggatgttgt agtccatctc ccagcacggg ccgagcccgt agccctcgtc ggcgtcaccg
    25021 atggcggtga tcacctcgcg gcggaccgcg taacagaagt cgccgaggct gtgaagggga
    25081 ccgagtgcgc ggacctcgcg gccgaaccgc tgcagcgtcc gtgccgacat ggcgcggaca
    25141 acggccaacc tctcgggcgg ggcggccact gatgcagacg cggccgggtc gatcggggac
    25201 caacccaagg cttgctcgtt ccaccccaga ttggtggacg gtccggccaa cccgcagcca
    25261 gaggtcttca aggcggcgag cagcagttcc agccagcgag ggccgaccag cgagccgctt
    25321 tcgagcaaca cgatcaccgc ggcatccgtc gccgccgcca gccggttgaa ggcggctgcc
    25381 gtgcctcgag cctcagcggt cggccactgg gtcagcgagg aaagctcgac atcaccgctc
    25441 agtgccgcgc gcgtctcggc gtcgggtccg tcgggcagca caacgaggtc ggcggacaga
    25501 tccgtgtgca aatgcaggaa ctggacggtc tcgcgcaaca tcgctggttg ggcgtgcacg
    25561 gtgacgccga tgcagacgcg ccggtccatc gaccgcatcg tgaccgcgac gcgatcaccg
    25621 ccccgtcaca gccacgtcac caccgtggcc gcggcccggg ccctcaccga agagccctga
    25681 caggcgttca tgtcgcggat ctcgacagcg gatcgcgcag ttccgcgaca tgaacggtcg
    25741 gggcgcgtca gaccgcgacc agctcgaaaa gtccgctcag gtggtcgtcg tcgagcagat
    25801 cgtccggcgc tatgggctcc agcgccggcg tgagcagctc gcggtaggtc gagagcaggt
    25861 cgagcagccg ttccatgatg ggacgcggct cgtcccagaa actggcgtgg acggagaggt
    25921 agagcggggg atggctggcc gcgaggtacg agcgcgatcc ctcgagcacg gacacctcgt
    25981 acccctcgat gtcgatcttc accagcccga gcggtcctgg cggcagcgtg ctgacgaacg
    26041 cctcgagggt cacacactcg accggccaag aggcgtccgc gtcgccgaac aagaggctgg
    26101 acatgctgtc gccacccacg gccgacgtga tgctcccgag acgggcgacc cctggccgct
    26161 cggcgatggc cacgggatgc acgacgatcc gctcggcgag tggcggattg agggcgatgt
    26221 tcgcctcgag gcaggctctc gctcgaccgt ccggctcgat cgcgtggcat ctgtccgtga
    26281 ggtgcgcggc atacagcgag agcgggccga tccacgcgcc gatgtccaca aatgtcagac
    26341 cagacgacac gaagtggtcg atgaccgcga acgagtgcgg ttcccagtcg ccgtcggtca
    26401 cccgctccca gaacgccgcg aactcgtcat cgacgacgcg gaaggaatgg cggccgaccc
    26461 ggacctcgat gagcggaggg taaccgggca ggcgtgaggc cgttgccggt ccgtcctcag
    26521 tagtggaagc gctggttgac gcgcccctcc tcgcgctcat cgacgaccga acgcgtggtc
    26581 gcgtcattgg tgacctcggc gccgatcacc tcccaccaca cacctgatcc tggcggggca
    26641 taggacttgt cgacgtaaca ggcgatcagt gtcgagccgt cgcggtcgcg cgccgcctta
    26701 agggcgtcgc gcacctcgcc gggcgtgcgg gcagcaaccg cggtgagacc gatgctctcg
    26761 gcgttcttga cgtagtcgat ctcgaggtac tcgcccttgt cgatctcgcc cgtggccgcg
    26821 tcccggacct tgaactcgtt gccgagcgag tgcccgacgt acgagcgctg gtggccgtgg
    26881 atcgactggt agccgtagtt gacgctcacc aacaccgtga gcttcgcccg ctcctgcatc
    26941 gccgtcacca gctccatcgg gtggagctga tagttgccgt cgccgagcag cacatagacc
    27001 tcaccgccac cgagggcgag ccgcacgccg atggcgccgg ggatgtcgta ccccatgcac
    27061 gagttgccga actcgaggtg cagcgtgcga ccacccgcgg cgtcgaacag cttggtcacg
    27121 tcgctcggca ccgtcccggc cgcgcacacc agcacgtcgc cgtcctgcat ctgctcgttg
    27181 accacgccga tcacctgggc ctgcgtcatg tcctcgccgg gacggtcgac gtagacctgc
    27241 tcacgcttgg tcgtctccca ttcgccggcc aagcgctggg ccttggccac ccagtcaccg
    27301 ttgacgctga cgccggcggc gcgcccagcg tccgtgagcg ccttcaacgc cagcttggcg
    27361 tcggcggtga tcggcaacgc gccgagcttg tacgcgtcgc ggccgttgac gttcacggac
    27421 acgaaccgca cgcccgggtc gtggaacgcc gagctcgacc cggtgacgaa gtcgtgcaga
    27481 cgggtgccga tgcacagcac caagtcagcc tcagccgcca cctcggccgc agacggggtg
    27541 ccgttgagtc cggtgcccga catggacagc gggttggccg agccgtcgcg cagcgcaccc
    27601 cgaccagcgt gggtctcgcc gaccgggatg ccgaacgccg tggcgaactc gttcagctcg
    27661 tcccacgcct cggcgtagtg caccccgccg ccggcgatga tgtagggccg cttggccgac
    27721 tcgagcagct cgatcgcctc gttgatccgc accgggtcgg gaaggcgccg ttcgatccgc
    27781 cagacgtggg gctcgaagaa cgacgtcggg tagtcgtagg cgtagccctg gatgtcctgg
    27841 ggaagggcaa gtgtgaccgc gccggtgtcg gccgggctgg tcatgatccg catcgcttcg
    27901 ggcagtgagg tgaggatctg ctcgggacgg gtgacgcggt cgtagaagcg gctcaccacc
    27961 cggaacacat ccgtgaccga catgtcctcc gagctgaggt gctcgatgcc ctgcagcacc
    28021 acgccgccgt accgggaggc gtagtagtcc gacggcagca acagcaccgg gatgcggcag
    28081 atcgtcgcgg tggcggcacc ggtcaccatg ttggtggcac ccggcccgat cgacgtcgtg
    28141 cacgccatgg tcgccttgcg ccgcttggcc cgggcgaagc cagtggaggc gtgcaccatc
    28201 gactgctcgt tgcggggctg gtggtagggc agatcctggc cgtactcgac cagcgcctgg
    28261 ctcagacccg acacgttgcc atggccgaag atcccccaga tcccctggat gaaccgttcc
    28321 tgctcgccgt cgtactcgct gaactgcacc tgcaggtact tgacgatcgc ctgggcgagc
    28381 gtgagccgtt gcttcttctc tgacgggtag aactccatgt ctgctccttg tgtgttcgta
    28441 gatcggcgtc accatcgagc accgaacaac ggcgcttcag tgcgcacggg gatcatcgag
    28501 tcgcgaaagc gggccccgac atcggtgagc cggcgcccga cggacagccg tgggcggtgc
    28561 cgtgactgcg ggcggtagcc gagcacccgg gccgtctcgc tcaggtccca gcggctgccc
    28621 acgttgtcgg acatcacgtt gacgacggcg aacccttcga acggtaccgt gcacgcgagc
    28681 tgcacagctt gtcgccagtc gtcgttgctc agccacatct gctgtcccca caggccgaac
    28741 ggcatgtgcg ggccggggtg attctcgccc ggctggcaga agccgattcg caacgccagg
    28801 aacgagaccc cgcgccgggc gagcacagcg ccggcgcgct cgatcaccaa cttcgacatg
    28861 ccgtaggcgt tgaccggccg tggcggggtg ctcggcgtca ggcgagtgcg ccggaaccgg
    28921 tgaccgccca acacccagtt cgtactggcg aacaccaccc ggtcgacgcc gtgacgctcg
    28981 gcggcgtcga gcacgagcag ggacaggttg aggttgttgg cgaccaccga atcccacgcg
    29041 gcagtcggcc gcatgtccgc ggcgaggtgg agcacgacgt cgacatcgtc gaagtaccgc
    29101 tcccacgacg cgtcgacgac ggacagatcg gcgagcgcga cctcgacgcc gaggtcgaca
    29161 tcggccggac gatcggcgat cccgatcaca acgatgtcgt cgagggcgcg caaggccgcc
    29221 accgccttgc gaccaaggtt gccggtgata ccggtgatca gcacacgtct ggccaaggtg
    29281 cggaccctcc gtcggcgacc ctactgaagc gcttcaccag gggcaagctg gggagttgaa
    29341 gcgactgaag cgcttcagta acgtgggcgc gtgcgccggc ccaccctcga tgacgtcgct
    29401 cgtcaggccg gcgtctcccg cacgctcgtc tccctgacct tgcgcggctt ggccggtgcc
    29461 ggcgacgaga cacgtcgaac gatcttcgcc gccgccgagg agctcggata cgtcccgcat
    29521 gccggcgctc gcaacctggc atcgcagtcg tcgcgagcga tcggcgtctt gctgcacgat
    29581 ctgcacaacg ccttcttcgc cgatgtcgtc gatggcctgc acgcctcgat gcgtgagacc
    29641 gggttccacg tgctgatggc gtcgggtgac gagctccagc tcgagcggtc ggtgctcgac
    29701 acgttccgga gctatcgcat ggacggagcc gtgctgatcg ggcccgaggt gcgcgagcgt
    29761 gagatcgagg cgttcggcca ggcgatccca accgtcgtcg tcggccggcc ggtgcggtcg
    29821 aaggcggtcg acgtgatcgt cagcgacgac cgcaagggcg cgatgctggc cgtcgaccac
    29881 ctccacgagc tcgggcatcg cgacatcgcc cacctcgacg gtggcacggt gcctggcggc
    29941 gccaacaccc gccgcagtgg atacgtcgag gccatgcgcc gacgcggcct cgcgcggtac
    30001 gtgcgcatcg ccggtggcgg gttcacccac gcccatggcg ccgacggtgt cgatcagctg
    30061 ctgcgccggc accgcccgcc gaccgcgatc ttcgccgtca acgacctggt cgctctcggc
    30121 gccatgcaac gcctcgccga gctcgggctc accacaccgg gcgacgtgtc gatcgtcggc
    30181 tacgacaaca gctcgctcgc cgcgcagcgc agggtcggcc tgacgagcat cgaccagccg
    30241 cgcttcgaga tcgggatgta cgcggcagcg ctgctgcagg agcggttcgc gggccgccac
    30301 caatcggccc ggcgtctctt ggcgccgaag ctcgtcgagc gcgagaccac cgcgcctctt
    30361 gatgatcacc agctgatcag gaagggagcc gaatgacact caccgacacg acgaccaggg
    30421 acgccctcgg acggctgctc gccgacggct acgtcgtgat cccgaacctg ctcgacgccg
    30481 cgaccacgga gaacctgcgc gcccgggtgc gggtgctgct cgaccaagag cgcgcccacc
    30541 ccttcgaccc cggcccggac gcgcccgaac ctgacgacga ccagctctcg tggtacgccc
    30601 acatctggga cctcgacgac gacgagaaga cgcgcctctc acagcgcctg ttgctgcgcg
    30661 agcacgacga gttcgacacc ccgtggccgg tctcgaacgc cgacgtgtgc atcagcttca
    30721 tccacatccc gacgctcttc gaccgcggtc gttcacagcg gatcttcaac ctgatcaaca
    30781 aggacgccgc cttcgccccg ctgctcgagc acccgatcgt cctgtcgatc gtcgaggacc
    30841 agctcggcct cgacgcggta ctgctcgacg tcagcgtcaa cgcggtcggc ccgcagtcgt
    30901 cgagcggtgg ctggcacgtg gactcgccgc tgacacaggt gccagaaccg ttcccgaact
    30961 tcacgctgag catgcagacg gcctggatgc tcgacgactt ccacgtcgag aacggcgcga
    31021 cccacgtcgc tcgcggcagc catttgacgc tgaggcaacc gccgcgcggc cggggcgacg
    31081 tggacgacga ggtggtcctc gagggtcccg ccggctcggt ggcgatctgg ctgtcgcaaa
    31141 cctggcaccg ccacggcgag aactcgaccg acgccatgcg acgcggagtc atcgtccagt
    31201 acgggcggtg ctgggtgaag cccttcgtcg acctgcgcac gccgatgacc gcggcacacg
    31261 ccgccgagct gtcgccccgg ctgcgctaca tgatggggtg caacgccaac gcgcccgtgc
    31321 ggggctgatc atcgcttgac ccgctccggg aggctgttcg cctcgtacgc cggcttcacg
    31381 atcctcgcct ggtacctgag cgcgatgggc gcggtgctac cgccgctgcg cgacgaggcg
    31441 gggaactggg ccggcgtgta cgccctcctg cccggggccg tgctgttcgg ctgggggctg
    31501 atcacgctcg cccgcggctc gagatcgacg agcgaccgag aaccgaacat cgccgtcggc
    31561 ctcaccgtgc tcacgatctc gatcggcgtc atgggcgtca ccggcgcgcc ggccatctcg
    31621 ttgatcggcg ccgcggtggc ggcgatcgct gccgcgcacg tgaaccggac gttgccgggg
    31681 atgctggcca cggcgagcgg gggcaggccg gtgcaaccgg tcttgatgcg agccaacgcg
    31741 ttctcgagcg tcgccgcgat catcgccccg ctggcggtcg gcgcgtcact ggcgatcggc
    31801 gcgggatgga tcaccggcta cgtcgtgccg atcgcgatcg gcgccgtgct ggtgtgcgct
    31861 ctggccatga gagccggcgg cccggagcgc tcgttcgaga cgatggatcc agcgccgctg
    31921 ccaccgttcc gtcactggtg gcgggcctgc gccgtgctcg gcgtggccgt cgtcgtcgag
    31981 ttctgcttca cctacttcgc cgtgacctac ttgcacgacg agcgcggcct ctcgaaggcc
    32041 gccgccgcag caggcggtgc cgcctggggc atcggcatgg cgatcggccg gttcgcgttc
    32101 agcgtgcgcc agcctccgcc ctcgatcgtc cccagcgcag cagtgatcct cgccggtttc
    32161 gtcctgttct ggggcgtccc caactcagcg gtcgccatca tcgggatcac gatcgccggc
    32221 ctcggggtgg cgccgctcta cccgagccgg atcatcatcc tgatggagcg cttcccgggc
    32281 gccacgcacg aaggttcgaa gcgggccgcg ctcgcgtcag ggtgggcctt gctcgtggcg
    32341 ccggcgctga tggtcagcct gcgtgccgcc agcaacgtgc gcgtcgccta cctcgtcgtg
    32401 cccgtgctgc tcgtcctgct ggtggctctc gcctcgcccc gctgggaaca ctcccgccat
    32461 ctggtcgccg cctgacgtcg ccgacacagc tccgggatcc tcggcgcacg agtggttcca
    32521 ctagtggagc gtttcggaag tgagtgtgat gcatcggcga gtcatcgggg ttcctcgcac
    32581 ggcgcgacga cgaagcaata gccctgcgct attgcgagga ggaggaacac agcgagggac
    32641 gccgagggcc ggcgagacat cacaggaatc tccgaaatgt tccactaggg ccggtagaag
    32701 atgtcctcct gaccgtcggg aagcgtgagc acgagccgga cgtcgccgac cacgggatcg
    32761 cggtccacca cgtacggcca gctttgcgga ccctgctgcg acccggtctg cggtgtcagc
    32821 gtcagcatgc cgttcgcggc ttggacgtcg cccgacgccc tgtctttgat ggtgcaatcg
    32881 ttcgatccct ggcacatgcc gagcgcgtag tcatagtggc cgtccgggct gaaaaggtaa
    32941 gtacgtttga tctgctgcga accggtcgaa tcgagccaat ggctcgtctc ccacgccccc
    33001 acgagctcag cgtcgagcgg tccaggcgag ccggtcggcg gcaccgggga aggctgcgaa
    33061 tcggtcacgg gagcagcggt tggcgccggc gaaacggtgt ccggaacaga gagcggcacc
    33121 ggtgaaacgg tgtccggaac cgacagtggc actggtgcca cggtgtccgg gaaggacggc
    33181 accaacgagg ccgatggtgt cgacgcgctg ctgccgcacg ccgccgaaag cgagagcacc
    33241 agcgcggcga gcgcaccagt cccgacgatg cgcggtgcct gcccccggcc gtgagcatgc
    33301 gcactcatgt gaacgctcgc aggaagatgc gcgattgact cgcggtcgcg tcatgagcga
    33361 tgtccagcga tccatcgtgt cgacctgggg tcatcccggt gtaggtcacg tcgatggtca
    33421 gcgaagcacc tgggcccagc gtggtgccgg gcgctggaac gccgctgagg gcgaagtcgt
    33481 ggtcgtcgac gccgatcttc tggagcgtga ccgggtcggt gccgtccgag ttgatgacca
    33541 actcgcccgt cggcggtgcg ccggagttca gtgtcagcaa ctcggctggc tgcatggtca
    33601 ggtggccacc gacagtccga cccgcgaccg cgagctcggc ccgcggccgc agcggatcgt
    33661 cggagtggat caccagggtc gccgcggctg gtgctccggc gaccgctgag gcgccgaagc
    33721 tggcttggac cgcgagctca ccgccaggat tgacgacggc ggggaagatc gagaggttag
    33781 gcacgccgaa cgcggctggg cccgtggtgt cgacgctgct gacggacagc ggggcgtcac
    33841 ccacgttccg gatccagaac gtacgagtca ccgtcgtgcc gggcgcggcc gaaccgaagt
    33901 cgagcagggt cagctcgggc aggagctgcg caccgcccgg atgggggagt ggaaacccgg
    33961 ggccgggatg caatggcggt cccaatggag gtcctgccgg cggaagcgcc ctaccggcga
    34021 ggagcaccac cggcgcctgg gcggtcgccg ccaatggaac gtccacgcgc tgctcgaacg
    34081 ggtacggcgt gtccgtcgca ctgctcacgt gagcggagag tgtcgcggtc agcggtcccc
    34141 gcgtggtcgg ggtgcacgag aggtagaccg gctgactctg cccgggcgtg agctgaccgg
    34201 gaaacgagag cgggatcgca aactgaccgt cgggcgacga gtcgatgatc cgcagttcgg
    34261 cgatgccgag ggaacgggcg cccacgttcg tcagcgtgac gctctgcccc gcgggccggc
    34321 cggcgcgaac gacgccgaag tcgaggctgg acggcgacac ctggagaacg ccgtgtggcc
    34381 cgagtggagt agccgacgtc gccaacggga cggcgaactg gccgacggca gggtcgttgc
    34441 aggtcaccga caccgtgccg gtgatcggcc cgggctgccg cggacggaat tgtgcgttca
    34501 cccacaggaa cgagttcggt gggaccgtga gcggaagcgg agtcgtgacg gagaaggccg
    34561 tcgtgccgcc gccgtcctgg ctggtggtga tcgtcaatcc tgagacgacg accggctgag
    34621 ccgtcttgtt cgtcaacccg actgccgtcg agctcgtgga gccctcgacc tgtggggtcg
    34681 gctcccagcg gtcgaccggg tgacggaggt tgtcggagac atacaggccg ccgagccagt
    34741 acggcaagcc gccatcgggg atctgcgcca ggccaccagg cggcacgcgg gccacgggct
    34801 gtccggccat cccgctggcc gtcacgagcg cgggggtcga gcgatagcgc gtcccgttgc
    34861 ggacgatcca gacctgcgtc ggggtgttct gctcggccat cagatggctg ccccgcggga
    34921 tcctgggcag gtcggtgaac gtcgcgtggg ggacggcgag cgtcggtgtc cgcgtggtcg
    34981 agtgttcggt ctcgttcgac gcgaggaaca ccgcgccccg caccatccag tacacctgat
    35041 ccgtgccggc atcgatcagc gacccggcgt ccggggcgta ggtgttcggg tcgaggacgc
    35101 cgtcggcgac ccaggcctcg gtcccgttgt tgctgaacgc cgacagtcgc gaccagaccg
    35161 agaccgtgcc gaacggcccg agcggcctga actcatgcgc ccagttgcgc caccagcgct
    35221 gcgcctgcgg atgcagtggg agcccgagct cggtgcgcct ggccagcagc tctctgatga
    35281 tccagcggta cgtccggacg gtggcgacgg gctcacgggt ctccacggcc cgcacgaacc
    35341 agtcgttctc gccgatgtag cgctcgaggg agtagctacg aatccggaac gtagcgatga
    35401 ggtcggacag ccgtcggaag tcgcggcgcg ccgagtcgag cgcaggctgc atctcgtcgc
    35461 ccgcccgcat ccatccgtag gcgctgctga tctcgatcaa cgactcgttc acctcggttc
    35521 cgccgtggac gtcgaatgtc ggcgcgatca ccgtgcagct gatgttcttc gcccgggccg
    35581 ccgcgatgtc gtcgacggtg acctcctgga gagcgatctg ggtgagctcg cgcattccca
    35641 cctcggccag cccggcgttg gacatgtcgt cgacgcgggg cagctcgagc gacgagcagc
    35701 agatcccgac gatgtcggtc acgccgttgc gcaccgcgat ctccatcggg atgatctcgc
    35761 ggatgcccgc gtcgacatag cgctcggctc cgatgacgat tggatccgcg aacaccggca
    35821 tcgcggccga agcgagcaca ccgcggcggg ggtcgacgtg ggtgctcagg cgcagatctc
    35881 gcacctgatc gcccagctca tcgaggaagt agttgccacg ctcgatgttg cgggcgatgt
    35941 cccggagggt cgccacacgc tgctggaccg tcgtcgcggt ttgccggcag atgacgcgac
    36001 cggcctggat cgcggcgatc gccgctcggt aacgagccga cagggccttg atcgcgttct
    36061 tgcggtctgt gcccagcggc tgcaggttct cgtcga
//
LOCUS       DATBFZ010000178         1524 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_447114, whole genome shotgun sequence.
ACCESSION   DATBFZ010000178 DATBFZ010000000
VERSION     DATBFZ010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1524
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447114"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..796
                     /locus_tag="VH419_05445"
     CDS             <1..796
                     /locus_tag="VH419_05445"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEY7043095.1"
                     /translation="FSFSNVELTGGEPFRFVGLENYQKLLADSDVWRSLTVTFVFAAI
                     WLPVTIAAPFFIAVLLNSPRLRGSSLFRVLVFLPYVVPVVAGVLIWSSMLLADTGWVN
                     EFLRALGWSNPPDWLQDPTWIYPGMTIVAIWTIGSAVIVNIAGLNGVPTELYDAAKID
                     GAGAWAQMRHITLPMVSPVIFYSLTLGVVGVLQFFLVPFILKNGTGEPGGTTLFYNLY
                     IYKNFFTFQHMSYGATLAWLLFLTTLVITVGLFASARRWVYYAGER"
     gene            797..>1524
                     /locus_tag="VH419_05450"
     CDS             797..>1524
                     /locus_tag="VH419_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043096.1"
                     /translation="MAAPAASMSSGGLFGRWRRPSAPGQSRVSSFVITLIAVCFIAAF
                     VSPMLRSLAISLKDREQITQLGAPLYPADPVTFTYEGRQYDVYQVPVDGTVKSLALVE
                     PGRRSSKFVDPANPDAGLITWEGSFRSLDRTWQFNAHFENFATAWSLLDYPRLLFNTV
                     VIATVGMIGVLLSCTLVAYGFARFRFPGRNFLFLLLISTIFLPTIVTVIPTYAIWVKL
                     GVVGSSNPVLAWAPLLVPTFFANA"
BASE COUNT          224 a          513 c          454 g          333 t
ORIGIN      
        1 gttttccttc tcgaacgtcg agctcacggg tggcgagccg tttcgattcg tagggctcga
       61 gaactaccag aagctgctcg ccgattcgga cgtatggcga tccctgacgg tcaccttcgt
      121 gttcgccgcc atctggctgc ccgtgacaat cgcagcgccc ttcttcattg cggtcctgct
      181 gaacagtccg cgactccgcg gctcgagcct gttccgtgtc ctggtgtttc tgccgtacgt
      241 ggtgcctgtt gtggccggtg tcctgatctg gtcgagcatg ctgctcgccg acaccggctg
      301 ggtgaacgag ttcctgcgcg cgctcggctg gtcgaacccg cccgactggc tgcaggatcc
      361 gacatggatc tacccgggca tgacgatcgt cgccatctgg acgatcggca gcgcggtcat
      421 cgtcaacatc gccggtctga acggggtgcc gaccgagctg tacgacgcgg ccaagatcga
      481 cggcgccggt gcgtgggcgc agatgcgcca catcacattg ccgatggtct cgccggtcat
      541 cttctactcg ttgacgctcg gggtggtcgg cgtcctgcag ttcttcctgg tgccgttcat
      601 cctcaagaac ggcaccggcg agccgggcgg aaccacgctc ttctacaacc tgtacatcta
      661 caagaacttc ttcacgttcc agcacatgtc gtacggggca actctcgcct ggctgctgtt
      721 cctgaccacg ctcgtcatca ccgtcggact gttcgcctcg gcacgacgct gggtctacta
      781 cgcgggggag cgctgaatgg cagccccggc cgcctcgatg agcagcggtg ggctcttcgg
      841 tcgctggcgg cggccaagcg cgccggggca aagccgcgtc agctcgttcg tcatcacgct
      901 gattgccgtc tgcttcatcg cggccttcgt ctcccccatg ctccgatcgc tggcgatctc
      961 actcaaggac cgcgagcaga taacgcagct cggtgcaccg ctgtatcccg ccgatccggt
     1021 cacgttcacg tacgaggggc ggcagtacga cgtctaccag gtccctgtcg atgggaccgt
     1081 caagagcctc gctctggtcg agccgggacg ccgcagctcc aagttcgttg acccggccaa
     1141 ccccgacgcg gggctgatca cgtgggaagg ctcgttccgc tcgcttgatc ggacatggca
     1201 gttcaacgcc catttcgaga acttcgccac ggcctggtcg ctgctggact atccgcgcct
     1261 gctgttcaac acggtggtca tcgccaccgt cgggatgatc ggtgtgctgc tctcgtgcac
     1321 cctcgttgcc tacggcttcg cgcgcttccg attcccgggt cgcaacttcc tcttcctgtt
     1381 gctgatctcc accatcttcc tgccaacgat agtgacggtc atcccgacct acgcgatctg
     1441 ggtgaagctg ggcgttgtcg gatcctccaa tccggtcctc gcctgggcac cgctgctggt
     1501 accgacgttt tttgccaacg cgtt
//
LOCUS       DATBFZ010000179         3593 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_1429061, whole genome shotgun sequence.
ACCESSION   DATBFZ010000179 DATBFZ010000000
VERSION     DATBFZ010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 3593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3593
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1429061"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="VH419_05455"
     CDS             complement(<1..347)
                     /locus_tag="VH419_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012918258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEY7043097.1"
                     /translation="MADGGHKLVIVESPAKARTISGFLGRGYVVESSIGHIRDLPNNA
                     SEVPAKYKGEPWGRLGVNVDDGFTPVYVVPRDKKSQITKLRSLLKDADELYLATDEDR
                     EGEAIAWHLYDEL"
     gene            complement(484..1977)
                     /locus_tag="VH419_05460"
     CDS             complement(484..1977)
                     /locus_tag="VH419_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEY7043098.1"
                     /translation="MLGELRRSLVVADFTVAGVTELIGAHAHEALARNETTPARQRTS
                     DGSPLATLTRLWLLQADVDESDLVRALPGLVDPLAASGLLERAGGQVRARLDVRPYAD
                     DDRDWWVVSDLTPGLDGVVGRMAADHVLGVSTASTSLAQLTMRTPVQRALDLGTGSGV
                     QLLHLSSHVADVVGTDVNPRCLDLAKLTLDLNEVAADVRAGDLFEPVAGERFDLIVSN
                     PPFVISPGDSERLAYRDSGLPGDEVVRRLVSAGADHLSDGGWLQALGNWVHLVGEPWS
                     ERVGRWVSATGCDAWVIEREVVDPPRYVEMWLADAGLQGAPDYVRRYDAWLRWFDEQH
                     VEAVGFGWLNLHRTGRTEPVVRLEEWPYAVDQPIGSHIAAWGRDVEAATAYGSSAHDV
                     TDTLLRQRLVRRSDVREERTGVPGDAEPQRIVLRQRRGLRRARSVSTVEAAFYGACDG
                     DLTVGQIAEAVAMLLGTDVTETTREVVAAARDLIATGYFTHENDPAD"
     gene            complement(1992..>3593)
                     /locus_tag="VH419_05465"
     CDS             complement(1992..>3593)
                     /locus_tag="VH419_05465"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY7043099.1"
                     /translation="PFIDGGWYWFDIEAAESRFVLHSADWSVQTDTTQPGTVSIGITT
                     YNRPDFCVDQLLKLSQEPEVLALLDEVIVVDQGNKKVRDHTSYAAAAEALGPKLRLIE
                     QGNLGGSGGFSRSMDEVTAKGTADYVLLLDDDVVCEPEGILRAVTFADLTKNPMLVGG
                     QMFSLYDRTVLHAYGETVAKWRWFWGPAPNTGQGHNFGQRSLRATKWLHRRVDVDYNG
                     WWMCLIPTQVIREIGLALPMFIKWDDADFGLRAAKKGFKTVTLPGVAVWHVPWHEKDD
                     TIDWQAYFHRRNRIIAALLHSPYDRGGRLVRESFETQVKHVFSMQYAPAEMGLMAIED
                     VLEGPQRMHRDVRLRLGELNRLRKQYDDSIQRTDLDQFPEAKRSKPPRKGRTPRAPEG
                     LLGRSKAMTWAGLRQLLPVREMSRTHPETNVPHIDLRWWLLAQQDSALVSSADGTSAA
                     WYKRKPPIARDLLRRSIAVHARLAREWPQLAKEWKQAVPELASPKRWAETFEESLDKD
                     SDKNDKDADKDSGRNAVTDVPKDAS"
BASE COUNT          559 a         1281 c         1166 g          587 t
ORIGIN      
        1 ttcagctcgt cgtacaggtg ccaggcgatc gcttcgccct cgcggtcctc gtcggtcgcg
       61 aggtagagct cgtcggcgtc cttgagcagc gaccgcagtt tggtgatctg tgacttcttg
      121 tcacgaggaa cgacgtagac cggggtgaag ccgtcgtcga cgttcacccc gaggcgtccc
      181 cagggttcgc ccttgtactt cgccggcacc tcgctggcgt tgttgggcag gtcgcggatg
      241 tgaccgatcg acgactcgac gacgtagccg cgtccgagga agccggagat cgtgcgcgcc
      301 ttggcaggcg actccacgat caccagcttg tgtccaccat cagccacggg ggatgctcct
      361 acgtcttcct gtagttcctg cgttcgtcga gatcttcgtt ccggagggta cgccgtggcg
      421 aggctgacac ggcgcaacga tgaacggtaa cccgtacgtc aaaccgagcg acaacatgac
      481 cgatcagtcc gcggggtcgt tctcgtgggt gaagtacccg gtcgcgatca ggtcgcgcgc
      541 tgccgcgacc acctcccggg tggtctcggt cacatcggtg ccgagcagca tggccactgc
      601 ctccgcgatc tggccgacgg tcaggtctcc gtcgcaggcg ccgtagaacg cggcctcgac
      661 cgtggacaca cttctggccc gccgcagccc gcgtcgctgg cgcaggacga tgcgctgcgg
      721 ttccgcgtca cctggcaccc cggtgcgttc ctccctgaca tcggagcgac gcaccagccg
      781 ctgccggagc agcgtatccg tgacgtcgtg cgcgctgctg ccgtacgccg tcgctgcctc
      841 gacgtcgcga ccccatgcgg cgatgtgcga gccgatcggc tggtcgacgg cgtacggcca
      901 ctcctcgaga cgcacgaccg gctcggtgcg gccggtcctg tgcaggttga gccagccgaa
      961 cccgacggcc tcgacgtgct gttcgtcgaa ccaccgcagc cacgcgtcgt accgccggac
     1021 gtagtcgggc gcgccctgga ggccggcgtc cgccaaccac atctcgacgt aacgtggcgg
     1081 atcgaccacc tcgcgctcga tgacccaggc gtcacatccc gtcgccgaga cccaccgacc
     1141 cacccgctcg gaccaaggct cgcccacgag atgcacccag ttgcccagcg cctgcaacca
     1201 gccgccgtcg ctcaggtgat cggcacccgc cgagacgagg cggcgtacga cctcgtcccc
     1261 gggaagtccg gagtcccggt acgcgagccg ctcgctgtcg ccgggcgaga tcacgaacgg
     1321 cgggttggac acgatcaggt cgaaccgctc ccccgcgacc ggctcgaaca ggtcccccgc
     1381 ccgaacgtcg gcggcgacct cgttgaggtc caaggtcagc ttggccaggt ccaggcaacg
     1441 cgggttgaca tccgtcccca cgacatcggc cacgtggctg ctgaggtgca gcaactgcac
     1501 ccccgagccg gttcccaggt cgagggcccg ctggacgggt gtgcgcatcg tcaactgggc
     1561 aaggctcgtc gatgcggtcg acactccgag gacgtgatcg gccgccatcc gaccaacgac
     1621 cccgtcgaga ccgggcgtca ggtccgacac cacccaccag tcgcggtcgt cgtcggcgta
     1681 cggccgaacg tcgaggcgcg cgcgcacctg cccacccgca cgctccagca gtccggatgc
     1741 cgccaacgga tcgaccagtc ctggcagcgc tcgcaccagg tcggattcgt cgacgtcggc
     1801 ttggaggagc cacagccgcg tcagggtcgc cagcggcgac ccgtccgagg tccgctgccg
     1861 cgctggcgtg gtctcgttgc gggcgagcgc ctcatgtgcg tgtgcaccga tgagctcggt
     1921 gacgcccgcg acggtgaagt cggcgacgac gagcgaccgc cggagctccc ccagcacccc
     1981 aggtgcgagg gtcaggacgc gtccttgggg acgtccgtca cggcgttcct gcccgagtcc
     2041 ttgtcggcgt ccttgtcgtt cttgtcggaa tccttgtcga gtgactcctc gaacgtctcg
     2101 gcccagcgct tgggcgacgc cagctccggc accgcctgct tccactcctt cgccagctgc
     2161 ggccactcgc gggccagccg ggcgtggacc gcgatcgacc gacgcaggag gtcccgcgcg
     2221 atcggaggct tccgcttgta ccaggcagcg ctcgtcccgt ccgccgagga caccagggcc
     2281 gagtcctgct gcgcgagcaa ccaccaccga aggtcgatgt gcggaacgtt cgtctcgggg
     2341 tgagtcctcg acatctcccg caccggaagc agctggcgca ggccggccca cgtcatcgcc
     2401 ttcgacctgc caaggagacc ctcgggtgcc cgcggcgtcc tgcccttgcg cggcggcttc
     2461 gagcgtttgg cctcggggaa ctggtcgaga tcggtgcgct gaatcgagtc gtcgtactgc
     2521 ttgcgcagcc gattgagctc gccgaggcgc agccgcacgt cacggtgcat ccgctgcggc
     2581 ccctcgagga cgtcctcgat cgccatcagg cccatctcag cgggggcgta ctgcatcgag
     2641 aacacgtgct tgacctgcgt ctcgaagctc tcgcgaacca agcgcccgcc acggtcgtac
     2701 ggcgagtgga gcagggccgc gatgatgcgg ttacgccgat ggaagtacgc ctgccagtcg
     2761 atggtgtcgt ccttctcgtg ccacggcacg tgccagacgg cgacacccgg cagggtcacg
     2821 gtcttgaagc ccttcttcgc agcccgcagt ccgaagtcgg cgtcgtccca cttgatgaac
     2881 atgggcagcg ccaggccgat ctcgcggatc acctgagtgg ggatgagaca catccaccag
     2941 ccgttgtagt cgacatccac acgccggtgc agccacttcg tcgcgcgcag cgaccgctgc
     3001 ccgaagttgt gaccctggcc ggtgttcggg gccggacccc agaaccagcg ccacttggcg
     3061 accgtctcac cgtaggcgtg cagcacggtc ctgtcgtaca ggctgaacat ctggccgccg
     3121 accagcatcg ggttcttcgt caggtcggcg aacgtcacgg ctcgcaggat gccttcgggc
     3181 tcacagacca cgtcgtcgtc gagcaacaag acgtagtccg cggtcccctt ggccgtcacc
     3241 tcgtccatcg aacgtgagaa cccaccggag ccaccgaggt tgccctgctc gatcagccgg
     3301 agcttcggac ccagcgcctc cgccgcagcg gcgtaggacg tgtggtcgcg gaccttcttg
     3361 ttgccctggt cgaccacgat gacctcgtcg agaagcgcca gcacctccgg ctcctgcgac
     3421 agcttgagca gctggtcgac acagaagtcg ggccggttgt aggtcgtgat cccgatcgag
     3481 accgtgcccg gctgagtcgt gtcggtctgg acgctccagt ccgccgagtg cagcacgaac
     3541 cggctctcgg ccgcctcgat gtcgaaccag taccagccgc cgtcgatgaa cgg
//
LOCUS       DATBFZ010000180         9216 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_1106533, whole genome shotgun sequence.
ACCESSION   DATBFZ010000180 DATBFZ010000000
VERSION     DATBFZ010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 9216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9216
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1106533"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..404)
                     /locus_tag="VH419_05470"
     CDS             complement(12..404)
                     /locus_tag="VH419_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043100.1"
                     /translation="MLVLVYSDDVNTRNEVMLALGKRPHPDLPDLEYVECATEPVVIA
                     SMDAGGVDLAILDGEAVPAGGMGICRQLKDEIFRCPPVLVLIGRPQDEWLATWSRADG
                     IVSYPLDPIDVAAAVAELMRRRLAAPSV"
     gene            complement(469..1422)
                     /locus_tag="VH419_05475"
     CDS             complement(469..1422)
                     /locus_tag="VH419_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020390395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEY7043101.1"
                     /translation="MTYLAHAGEGDHVPAISPERVFTAWTFDWLAVLLVVLVAAAYLI
                     GVRRLRARGDRWPVSRTLSFCGLGLGSLVVATCSSLATYDTVLLSMHMVQHMVLSMVA
                     PVFLALGAPVTLALRTLPRRGRQILLWGLHSRVAAVLAFPPVAFVLFVASPWVLYFSG
                     LYEETLRSPFLHDMLHLHFVAVGCLFFWPLLGIDPVPGRVAYPFRMLMVFATLPFHAF
                     LGVTIMTMDTLIAADWYLSLDRTWPPLPMDDQQIAGSILWASGDLIGLVIFGVLFVQW
                     AKQSQREAERIDRQLDREEAEAAAAAARRPQAPERRTSSPR"
     gene            1608..2213
                     /locus_tag="VH419_05480"
     CDS             1608..2213
                     /locus_tag="VH419_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014598467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-copper oxidase subunit III"
                     /protein_id="HEY7043102.1"
                     /translation="MHGQHDRPSMVSVGTIVWLASELMFFAALFACYFTIRSLEPALW
                     AQETEKLNVPFASVNTTVLVLSSVTCQLGVFAAERGQVGREGSVFAVPKWGFREWFVL
                     TYVMGSFFIGGQVYEYFELIKENVTISSSPYGSVFYLATGFHGLHVTGGLIAFLFVLG
                     RTFVARTFTHEQAVTAIVVSYYWHFVDVVWIGLFATIYLLQ"
     gene            2305..3129
                     /locus_tag="VH419_05485"
     CDS             2305..3129
                     /locus_tag="VH419_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007078109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY7043103.1"
                     /translation="MTFLSTRRRHPLAGTVVVLLGLVVTAALYAAFRPAVASTSTKAD
                     DDLIEQGRQLFVVGCSSCHGLNGEGVVTKGGNDYGPPLIGVGAAAVDFQVGTGRMPLQ
                     QPNTQAPRKPRTYSDEEVAALAAYVASLGPGPAIPDESDLDTSNANVARGMELFLANC
                     SACHNFAGRGGALRSGRYAPGLMDTTAKHIWEAMATGPQQMPVFSDGVMTPDDKRDII
                     AFVEHLQDQPAYGGDPLGSRGPVTEGIVGWVVGIGGLILVAVWIANNGARARRKKV"
     gene            3294..4322
                     /locus_tag="VH419_05490"
     CDS             3294..4322
                     /locus_tag="VH419_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002532212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEY7043104.1"
                     /translation="MRGEIEGPVPDPGLPPHEPRPQDVDPALELRAERQVAGMFLVAA
                     LLIVAFCVFYFVFEAGPDGELFFGWGASNLFLGLSLGFALLLIGFGLIHWARKLMGNE
                     EIIEYRHGASSSAEDREETLAAFYQGVEESGFTRRRLVRNSLLTSLGLLGIPAVIMLR
                     DLGPLPGNKPNETVWRRGVRVVNDVSGNPIKPEDMEIGQLVNGEPALFFEQEDADGNV
                     VPAEYEGAALQAAKAKAAVIMVRMQPKDITPSPEAKDWGIDGILCFSKICTHVGCPIS
                     LWEQQTHDVLCPCHQSTFDLADNAKVIFGPAARPLPQLPLIVDDEGYIAAASDFTQPV
                     GPSFWERG"
     gene            4327..6090
                     /locus_tag="VH419_05495"
     CDS             4327..6090
                     /locus_tag="VH419_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007078111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase cytochrome b
                     subunit"
                     /protein_id="HEY7043105.1"
                     /translation="MSTTTSTNASATSSQARPGQKKNPAAGPVEWLDDRVGLAGLAKK
                     QLRKVFPDHWSFMLGEIALWSFVVLLLTGVFLTFWFDPSMQEVTYDGAYVPLRGVRMS
                     SAYASTLDISFNVRGGLLMRQMHHWAANLFLAAMTIHMLRIFFTGAFRKPRELNWLVG
                     LGLLQLGIIEGFAGYSLPDDLLSGTGLRIAEGLMRSIPVVGTYVSFFVFGGEFPGDIF
                     IPRLYAVHILLIPGLLIGLISAHMLLLVYHKHTQWPGPGRTNDNVVGYPLLPVYAAKA
                     GGFFFIVFGFTALLGALAQINPIWMYGPYDPSKVTADAQPDWYMGWLEGGLRIMPNWE
                     THLWGFTVSWNVFVPGLLLMGLLFTVLALYPFVEHWVTGDKREHHLLDRPRNHPTRTG
                     FGVAGITAYGVLWAAGGNDLIGPFFHLSIYQITWTARIAFFVAPVLAFMIARRWCLSL
                     QRHDRETVLHGYETGVIVRSPTGEYTEIHRQINPIRTYNMTLHDQPRPAELPAGEDVN
                     GVAARGQAVERVRVRLSRFWYGDDVVKPSRGELEEAAHHLQDVDHSTDAFVPHDEHKG
                     IETGGQVGGDEFELEQPSPQR"
     gene            complement(6332..7567)
                     /locus_tag="VH419_05500"
     CDS             complement(6332..7567)
                     /locus_tag="VH419_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEY7043106.1"
                     /translation="MSARGMRTRLTVVAVATTALCLALTGCNSNDADNNASGSGDRAA
                     NNNAPEDAQPEAPKTSEAAITTNVSRGKVATVDTAVKVKVADGTFDAVTMRVVSGKPV
                     SGSFNADKTAWTAGSLLEPGTRYVVTSRATDANGLSKASRTPFRTEPLTLDQQTYASI
                     TPLQGETVGVGMPVIVNFDIPVKNHAAFEKKMTVTASPKTVGSWNWISDQEVHWRPKA
                     FWKPGTHVSVNVDVNGLNAGNGIYGQMDRHVSFNVGDSVILRPNLQTDQMPVYINGKL
                     ARTIPITGGKAGFETRSGIKLIVEKFEVKRMDAATVGIQPGSPEYYDIPDVQYAQRVT
                     FTGEFLHAAPWSVYAQGSYNVSHGCVGMSTDNAAWLFGLTHRGDPVEVTGTSRPLESG
                     NGWTDWNESFAEYKQGSAL"
     gene            complement(7840..8235)
                     /locus_tag="VH419_05505"
     CDS             complement(7840..8235)
                     /locus_tag="VH419_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002527710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 4"
                     /protein_id="HEY7043107.1"
                     /translation="MKIEAWIFGVNAAFFALVAPTYWFISDDPTGTAALVMTTLLAAL
                     VTLYLGFHANKMDPRPEDRKDAEVVDGAGELGFFPPYSWWPLWCGLTLAVSVLGIIFG
                     WWLFLIGVGAGSIALIGWVFEFYRGEHAH"
     gene            complement(8232..>9216)
                     /locus_tag="VH419_05510"
     CDS             complement(8232..>9216)
                     /locus_tag="VH419_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007078115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEY7043108.1"
                     /translation="TEIIPVFSRKPIFGYKGLVGATLAIAALSVAVWAHHMFVTGAIN
                     LPFFSFMTFLIAVPTGVKFFNWIGTMWGGSVSFDTPMLWAVGFLVTFLFGGLTGIILA
                     SPPLDFQLSDSYFVVAHFHYVVFGTVVFAMFGGFYYWWPKLTGRFLDERLGKIHFWLL
                     FVGFHTTFFIQHWLGVEGMPRRIADYRVTDGFTGMNEISSIGAFVLGASTIPFLYNVW
                     KSRNAPLVESDDPWGWGRSLEWATSCPPPRHNFERLPRIRSESPAFDLHHPEIALMEL
                     EHNGRLHTDEQVADAPDVTGREEHLEELKDGAPPQGADPHPVITDDEEGRS"
BASE COUNT         1540 a         3068 c         3118 g         1490 t
ORIGIN      
        1 agggtcggtc atcagacgct cggggcggcc aggcgtcggc gcatgagctc ggcgacggcg
       61 gcggccacgt cgatcgggtc gagcgggtag gagacgatgc cgtcggcgcg ggaccaggtg
      121 gccagccact cgtcctgggg gcggccgatc aggaccagga ccggcgggca gcggaagatc
      181 tcgtccttga gctggcggca gatccccatc ccgcccgccg gcaccgcctc cccgtcgagg
      241 atcgccaggt cgacaccgcc ggcgtccatc gacgcgatca cgaccggctc ggtggcgcac
      301 tcgacgtact cgagatcggg caggtcggga tgtggtcgct tgccgagcgc cagcatgacc
      361 tcgttgcggg tgttcacgtc atcgctgtac accagcacca acatgtcgtg acctgcctcg
      421 tcgtaccggg acgcgtgcgg acagccagcg tcgtgtgctg ggccgatgct accggggcga
      481 cgacgtacga cgctcgggcg cctgcggtcg tcgcgcggct gcggcggctg cctccgcctc
      541 ctcgcggtcg agctggcggt cgatgcgctc ggcctcccgc tggctctgtt tcgcccactg
      601 cacgaagagc acgccgaaga tgacgagccc gatgaggtcg ccactcgccc acaggatcga
      661 ccccgcgatc tgctggtcgt ccatgggcag cggcggccag gtccgatcca gcgacaggta
      721 ccagtcggcg gcgatcaacg tgtccatcgt catgatcgtg acgccgagga atgcgtggaa
      781 cggcagcgtc gcgaacacca tcagcatccg gaacgggtac gccaccctgc cggggaccgg
      841 gtcgatcccc agcagcggcc agaagaacag gcagcccaca gcgacgaaat gcaggtgcag
      901 catgtcgtgc aggaacggcg atctcagcgt ctcctcgtac aacccggaga agtacaagac
      961 ccacgggctg gcgacgaaga gcacgaacgc caccggcggg aacgcgagca cggcggccac
     1021 ccggctgtga agtccccaca gcaggatctg gcgcccccga cgtggcagcg tgcgcagcgc
     1081 gagcgtcacc ggggcgccga gcgccaagaa caccggcgcc accatcgaca acaccatgtg
     1141 ctggaccatg tgcatgctga gcagcacggt gtcgtacgtc gccagcgacg agcaggtggc
     1201 gaccacgagc gagccgagtc cgagcccgca gaacgacagc gtccgtgaca ccggccagcg
     1261 gtcgccgcgg gcgcgcagtc ggcgtacacc gatcaggtac gccgctgcca cgagcacgac
     1321 gaggagcacc gccagccagt cgaaggtcca cgccgtgaag acacgctccg ggctgatcgc
     1381 cggcacgtgg tcgccttcgc cggcgtgcgc caggtaggtc acccccacag cgtaggtcgc
     1441 cggccggggc tcgtggaggc cgaggctgga acccgtccca ggcgaatgga ccgaggtgat
     1501 cggccacctc gatcggcaga aatcgttcgg ggggtcgctg tcccataccc cgaccctgac
     1561 gacataatgg ccgcgtggcg actacgacgg cgatccccgt atcccggatg cacggccagc
     1621 acgatcggcc gagcatggtc agtgtcggca ccatcgtctg gcttgccagc gagctgatgt
     1681 tcttcgcggc actgttcgcc tgctacttca ccatccgcag tttggagccc gcgctgtggg
     1741 cccaggagac ggagaagctc aacgttccgt tcgcctcggt caacacgacg gtcctggtgc
     1801 tgagctcggt gacgtgccag ttgggcgtgt tcgcggccga gcgggggcag gtgggacgcg
     1861 aaggcagcgt gttcgccgtc ccgaagtggg gttttcgcga gtggttcgtg ctcacgtacg
     1921 tcatgggctc gttcttcatc ggcggccagg tgtacgagta cttcgagctg atcaaggaga
     1981 acgtcacgat ctcgtcgagt ccgtacggct cggtcttcta cctcgccacc ggcttccacg
     2041 ggctgcacgt gaccggcggg ctcatcgcct tcctgttcgt gctcgggcgg acgttcgttg
     2101 ctcgcacgtt cacccacgag caggcggtga ccgccatcgt cgtctcgtac tactggcact
     2161 tcgtcgatgt ggtctggatc ggcctgttcg cgacgatcta cctgcttcaa tgacctcatc
     2221 cgtcccggcg gcacggctca cgcggtccgt cggcggcacc cgtccaacag cggctgacgt
     2281 accggagaga agggactcga cctcgtgacg ttcctgtcga cacgacgccg gcatccgctt
     2341 gcgggcacgg tcgtcgtgct gctcggtctc gtggtcaccg cggcgttgta cgccgcgttc
     2401 cgtcctgccg tcgccagcac gtcgacgaag gcggacgacg acctcatcga gcagggccgc
     2461 cagctgttcg tcgtggggtg ttcgtcgtgc cacgggttga acggtgaagg cgtcgtcacc
     2521 aagggcggga acgactacgg gccgccgctg atcggggtcg gggctgccgc cgtcgacttc
     2581 caggtcggga ccggccggat gcccctgcag cagccgaaca cgcaggcgcc gcgcaagccg
     2641 aggacgtact ccgacgaaga ggtcgccgca ctggcggcgt acgtcgcctc actcggtccg
     2701 ggccccgcca tccccgacga gtccgacctc gacaccagca acgccaacgt cgcgcgcggc
     2761 atggagctct tcctcgccaa ctgctcggcc tgccacaact tcgccggcag gggcggcgcc
     2821 ctccgcagcg gccggtacgc ccccggcctg atggacacga ccgccaagca catctgggag
     2881 gcgatggcca ccgggcccca gcagatgccg gtgttctccg acggggtcat gacgcccgac
     2941 gacaagcgcg acatcatcgc gttcgtcgaa cacctgcagg accagccggc gtacggcggt
     3001 gacccgctcg gaagccgcgg accggtcacc gagggaatcg tcgggtgggt cgtcggcatc
     3061 ggcggcctga tcctggtggc ggtgtggatc gccaacaacg gcgcgcgggc acgaaggaaa
     3121 aaggtatgac cgacgacaag agcccagccg acggcttcga cgagagccgg ggtggcggcg
     3181 acggcgcagg cccgggacgc ggcagcgacg ggtacggcac cggtgcggag cgggccggca
     3241 aggacaaccc ggaggactac cacgggtctc agccggtgca cgatcctgcg ctgatgcgcg
     3301 gcgagatcga ggggccggtc cctgaccctg gcctgcctcc ccacgagcca cgcccgcagg
     3361 acgtcgaccc tgcgctcgag cttcgggccg agcgccaggt cgccggcatg ttcctggtcg
     3421 cggccttgct catcgtcgcg ttctgcgtct tctacttcgt gttcgaggcc ggccccgacg
     3481 gtgagctctt cttcggatgg ggcgcctcga acctgttcct cgggctgagc ctcggcttcg
     3541 ccctgctgct catcggcttc gggttgatcc actgggcgcg caagctcatg ggcaacgagg
     3601 agatcatcga gtaccggcac ggcgcgtcct cgagtgcaga ggatcgagag gagacgctgg
     3661 cagcgttcta ccagggcgtc gaggagagcg gcttcacccg gcgcaggctg gtccgcaaca
     3721 gcctgctgac gagcctcggc ctgctgggca tcccagccgt gatcatgctc cgagacctcg
     3781 gcccactgcc aggcaacaag ccgaacgaaa cggtctggcg gcgcggcgta cgcgtggtga
     3841 acgacgtgtc aggcaacccg atcaaacccg aggacatgga gatcggtcag ctggtcaacg
     3901 gtgaaccggc cctgttcttc gagcaggagg acgccgacgg aaacgtcgtg ccggcggagt
     3961 acgaaggagc cgcgctgcag gcggccaagg cgaaggccgc ggtcatcatg gtgcgcatgc
     4021 agccgaagga catcacgccg agcccggagg cgaaggactg gggcatcgac ggcatcttgt
     4081 gcttctcgaa gatctgcacc cacgtcggct gcccgatcag tttgtgggag cagcagacgc
     4141 atgacgtgct gtgcccctgt caccagtcga cgttcgacct ggcggacaat gccaaggtga
     4201 tcttcggacc ggccgcgcgg ccgttgccgc agctgcccct gatcgttgac gacgagggct
     4261 acatcgccgc ggcgtcagac ttcacccagc ccgtcggccc cagcttctgg gaacgaggtt
     4321 gatcacgtga gcacgacgac gagtacgaac gcatcggcca cctcgtcgca ggcgcgcccg
     4381 gggcagaaga agaacccggc cgccgggccg gtcgagtggc tcgacgaccg ggtcggcctc
     4441 gcgggcctgg ccaagaagca gctccggaag gtcttccccg accactggtc gttcatgctc
     4501 ggcgagatcg cgctgtggag cttcgtcgtc ttgctgctca ccggcgtctt cctgaccttc
     4561 tggttcgacc cgagcatgca ggaggtcacc tacgacggag cgtatgtccc gctccgcggc
     4621 gtccgtatgt cgtcggcgta cgcgtccacg ctcgacatct ccttcaacgt gcgtggcggc
     4681 ctgttgatgc ggcagatgca tcactgggcg gcgaacctgt tcctggccgc gatgacgatc
     4741 cacatgctgc gcatcttctt caccggcgcc ttccgcaagc cgcgtgagct caactggctg
     4801 gtcggtctcg gcctcctcca gctcggcatc atcgagggct tcgccggata ctcgctcccc
     4861 gacgacctgc tgtccggcac cggcctgcgg atcgccgagg gcctgatgcg gtccatcccg
     4921 gtggtgggga cgtacgtctc gttcttcgtc ttcggcggtg agttcccggg tgacatcttc
     4981 atcccacggc tgtacgccgt gcacatcctg ctgatccctg gactgctcat cgggctgatc
     5041 tctgcccaca tgctgctgct cgtgtaccac aagcacaccc agtggcccgg ccccggccgg
     5101 accaacgaca acgtcgtggg ctacccgctg ctccccgtgt acgccgccaa ggcgggtggc
     5161 ttcttcttca tcgtgttcgg cttcacggcg ctgctggggg cgctggcgca gatcaacccg
     5221 atctggatgt acgggcccta cgacccgtcc aaggtcacgg ccgacgccca gcccgactgg
     5281 tacatgggct ggctggaggg tgggctgcgc atcatgccga actgggagac ccatctgtgg
     5341 ggcttcacgg tctcgtggaa cgtgttcgtc cccggcctgt tactgatggg gctgctgttc
     5401 acggtgctgg cgttgtatcc gttcgtcgag cactgggtca ccggcgacaa acgtgagcac
     5461 catctgctcg accggccgcg caaccacccg acgaggaccg gcttcggtgt tgccggcatc
     5521 acggcgtacg gcgtgctgtg ggccgctggc gggaacgacc tgatcggtcc gttcttccac
     5581 ctctcgatct accagatcac ctggaccgcg cggatcgcgt tcttcgtggc gccggtcctc
     5641 gcgttcatga tcgccaggcg ttggtgtctg tcgctgcagc ggcacgaccg cgagaccgtg
     5701 ctccatgggt acgagaccgg cgtcatcgta cggtcgccaa cgggtgagta caccgagatc
     5761 caccgccaga tcaacccgat ccgtacgtac aacatgacgt tgcacgacca gccgcggccg
     5821 gccgagctgc ccgccggcga ggacgtgaac ggtgtcgccg cgcgcggaca ggccgtggag
     5881 cgggtcaggg tgcggttgtc gcggttctgg tacggcgacg acgtcgtgaa gccgtcgagg
     5941 ggcgagctcg aagaagcggc ccatcaccta caggacgtcg accactcgac cgacgcgttc
     6001 gtcccgcacg acgagcacaa gggcatcgag accggcggcc aggtcggcgg cgacgagttc
     6061 gagctcgagc agccctcccc gcagcgttag cccaaacccg ttagcccaaa cccccagcgc
     6121 gcacctcgcc gagaggtcct cgccgaggtg gcaggtttcg ttggagacac gccggtccgc
     6181 gtgacgcgtt tgccacctcg gcggaacggg cgggaagcga tgagacgccc cgcgtcctca
     6241 cagcaggacg cggggcgctg cgacaaacgt agccgagctc ggtcagaccc gcagaccatt
     6301 cggtccacga ccagccagga tggtcagatg gtcagagggc ggagccttgc ttgtactccg
     6361 cgaacgactc gttccagtcg gtccagccgt tgccgctctc gagtgggcgg ctggtgccgg
     6421 tcacctcgac cgggtctccg cgatgcgtca ggccgaacag ccacgcggcg ttgtcggtgc
     6481 tcatgcccac acacccgtgc gacacgttgt acgagccctg tgcgtacacc gaccacggtg
     6541 cggcgtgcag gaactcgccg gtgaaggtca cccgctgggc gtactggaca tcggggatgt
     6601 cgtagtactc cgggctgccc ggctggatgc cgaccgtggc cgcgtccatc cgcttgacct
     6661 cgaacttctc gacgatcagc ttgatgccgc tgcgggtctc gaaaccggcc ttgccgccgg
     6721 tgatcgggat cgtccgggcc agcttgccgt tgatgtacac cggcatctgg tccgtctgca
     6781 ggttgggccg caggatgacg gagtcgccga cgttgaagct cacgtggcgg tccatctgcc
     6841 cgtagatgcc gttgccggcg ttgaggccgt tgacgtcgac gttgacgctc acgtgtgtgc
     6901 cgggcttcca gaaggccttc ggccgccagt ggacttcctg gtcgctgatc cagttccagc
     6961 tcccgaccgt cttgggcgac gcggtgacgg tcatcttctt ctcgaacgcg gcgtggttct
     7021 tgacggggat gtcgaagttc acgatgaccg gcataccgac acccaccgtc tcaccctgca
     7081 gcggcgtgat gctggcgtac gtctgctggt cgagcgtgag cggctcggtg cggaacggcg
     7141 tacggctggc cttggacagc ccgttcgcgt cggttgcccg gctggtcacg acgtagcggg
     7201 tgcccggctc gagcagggag ccggccgtcc acgctgtctt gtcggcgttg aacgaaccgg
     7261 agaccggctt gccggacacg acgcgcatcg tcacggcgtc gaaggtgccg tcggccacct
     7321 tgaccttcac cgcagtgtcg accgtggcga ccttgccacg ggagacgttc gtggtgatcg
     7381 cggcctcgga ggtcttcggg gcctccggct gggcgtcttc cggagcgttg ttgttggcgg
     7441 ctcggtcacc ggagcccgag gcgttgttgt ccgcgtcgtt gctgttgcat ccggtcaggg
     7501 ccagacacag cgccgtggtc gcgactgcga ccacggtgag gcgggtacgc atcccccgtg
     7561 cggacataaa cgaaactccc aggtgtacgg ctggcgtggg cgcgtcaagg gtaacaaggc
     7621 aggcaggcgc ccgcgaaata ccgtctcagc cctgcgcgcc gccccggaca aaccgggtca
     7681 gatcctcatg agcaccgaag gcaggctcaa ccagagccaa ccttcagtgg cgcttcaggt
     7741 ggatgcatgg caaggcggag gaggaagccg atacccaaag cgaagcgtat cggcgacgac
     7801 gacaacgcag ccaggcgccg cctgggcgtt ccggaggcgt cagtgggcgt gttcgccgcg
     7861 atagaactca aatacccacc cgatgagcgc gatcgagccg gcgccgacgc cgataaggaa
     7921 cagccaccag ccgaagatga tgccgagcac actgacggcc aaggtcagac cgcaccacag
     7981 cggccaccac gagtacggcg ggaagaagcc gagctcgcct gctccgtcga cgacctcggc
     8041 gtccttgcgg tcttccggac ggggatccat cttgttcgcg tggaacccga ggtagagcgt
     8101 gaccagcgcg gcgagcagcg tcgtcatgac gagcgccgcg gtcccggtcg ggtcgtcgct
     8161 gatgaaccag tacgtcggtg cgacgagtgc gaagaaggcg gcgttgaccc cgaagatcca
     8221 ggcctcgatc ttcacgagcg gccctcttcg tcatcggtga tcaccggatg cggatctgcg
     8281 ccttgtggtg gcgccccatc tttcagttcc tccagatgtt cctcgcgtcc ggtgacgtca
     8341 ggcgcgtcgg cgacctgctc gtcggtgtgt agtcgcccgt tgtgctcgag ctccatcagc
     8401 gcgatctccg ggtgatgcag gtcgaacgcc ggactctcgg agcggatgcg cggcaaccgc
     8461 tcgaagttgt gccgtggcgg cggacacgag gtagcccact cgagggaccg gccccagccc
     8521 cacgggtcgt cggactccac cagcggcgcg ttgcggctct tccacacgtt gtagaggaac
     8581 gggatcgtgg acgcgccgag cacgaacgcc ccgatcgacg agatctcgtt catgccggtg
     8641 aagccgtcgg tgacccggta gtcggcgatg cgacgcggca tgccttcgac gccgagccag
     8701 tgctgaatga agaacgtcgt gtggaagccg acgaagagca gccagaagtg gatcttgccc
     8761 agccgctcgt ccaggaagcg gccggtgagc ttcggccacc agtagtagaa cccgccgaac
     8821 atcgcgaaga ccacggtgcc gaacacgacg tagtggaagt gcgcgacgac gaagtacgag
     8881 tcggacagct ggaagtccag cggcggcgac gccaggatga tcccggtcag tccgccgaac
     8941 aggaacgtca cgaggaagcc gaccgcccag agcatgggcg tgtcgaacga cacggacccg
     9001 ccccacatcg tgcctatcca gttgaagaac ttcacgccgg tgggcaccgc gatcaggaac
     9061 gtcatgaacg agaagaacgg caggttgatc gcaccggtca cgaacatgtg gtgcgcccac
     9121 acggccacgg acagcgcggc gatcgcgagg gtcgcgccga ccagaccctt gtagccgaag
     9181 atcggcttgc ggctgaacac cgggatgatc tcggtg
//
LOCUS       DATBFZ010000181         2153 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_120687, whole genome shotgun sequence.
ACCESSION   DATBFZ010000181 DATBFZ010000000
VERSION     DATBFZ010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120687"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /locus_tag="VH419_05515"
     CDS             complement(<1..456)
                     /locus_tag="VH419_05515"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY7043109.1"
                     /translation="MTTITTPIPKPRTSQRVSLGVAAQDTFAITGRNLLTITRLPQLL
                     VFSTIQPIIFVLMFRYVFGGAVQIPGLTVPYVDYLMPGIFVQTVAFGATNTAVGLAED
                     VHKGLIERFRSLPMARSAVLAGRTVADLVRDAFVVALMIVVGFIVGFRIH"
     gene            complement(453..1484)
                     /locus_tag="VH419_05520"
     CDS             complement(453..1484)
                     /locus_tag="VH419_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY7043110.1"
                     /translation="MTVTPPSLSGADPAIRVEGLIKRFGSVEALSGVDFAVPSGAVLG
                     LLGPNGAGKTTAVRILTTVLRPDGGQATVLGLDVCQHPQTVRELIGLAGQYAAVDENL
                     TGRENLRLVGRLTHQTPSFARSRADELLERFDLTDAADRPARTYSGGMRRRLDLAAAL
                     VARPTVLFLDEPTTGLDPASRNEVWSMINELVAQGTTLLLTTQYLEEADRLATSIVVI
                     DHGRVIAEGTAAELKRNMGETILEFGLADSHAAARAQAVLKVVGGTSLDGDGRTVEVK
                     VASTRDVFEAVRVLDGEGLYPNTFVVREPTLDDVFLSLTGHRAEDSPAEEPVVAGAGG
                     RVKPAGGAS"
     gene            1586..2041
                     /locus_tag="VH419_05525"
     CDS             1586..2041
                     /locus_tag="VH419_05525"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY7043111.1"
                     /translation="MVDVSAKRRPSRARDDLADDAWQLMAGFFFEIGMRHMSESRQAT
                     GLNPGAIKALQSLDPDQPRTMGSLAEAWHCDASNVTWLVDRLEERGLVERRTLPTDRR
                     VKTVVLTDSGKALRNDVDAIWAKAPEQFRALDRDSLSGLIEVMRKLRPS"
     gene            2078..2149
                     /locus_tag="VH419_05530"
     tRNA            2078..2149
                     /locus_tag="VH419_05530"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2111..2113,aa:Gly,seq:tcc)
BASE COUNT          365 a          685 c          764 g          339 t
ORIGIN      
        1 atggatccgg aacccgacga tgaagccgac cacgatcatc aacgcgacaa cgaacgcgtc
       61 gcgcacgagg tcggccacgg tccggcccgc gagcaccgcc gagcgggcca tcggcagcga
      121 ccgaaagcgc tcgatcaggc ccttgtgcac gtcctcggcc aggccgacgg cggtgttggt
      181 ggcaccgaag gcgaccgtct gcacgaagat gcccggcatc aggtagtcga cgtacggcac
      241 cgtgagaccc ggaatctgga ccgccccgcc gaagacgtac cggaacatga ggacgaagat
      301 gatcggctgg atggtggaga acaccagcag ctgcgggagc cgggtgatcg tcaaaaggtt
      361 tcgcccggtg atggcgaagg tgtcctgcgc ggcgacgccg agcgaaactc gttggctcgt
      421 gcgcggcttg gggatcggcg tggtgatggt ggtcatgacg cacctcctgc tggcttgacc
      481 cggccacctg cgccggcgac cactggttcc tccgctgggg agtcttcggc ccggtggccg
      541 gtgaggctca ggaagacgtc gtcgagcgtc ggctcgcgta cgacgaacgt gttcgggtag
      601 aggccctctc cgtcgaggac gcgcacggcc tcgaacacgt cacgcgtgct cgccaccttg
      661 acctcgacgg tgcggccgtc gccgtcgagg ctggtgccac cgacgacctt cagcaccgcc
      721 tgggcgcggg cggcggcgtg cgagtcggcc agtccgaact cgaggatggt ctcacccatg
      781 ttccgcttga gctcggccgc ggtgccctcg gcgatcaccc gaccgtggtc gatgacgacg
      841 atcgaggtcg ccagccgatc agcctcttcg agatactgcg tcgtgagcag cagggtcgtt
      901 ccttgagcga ccagctcgtt gatcatgctc cagacctcgt tccggctcgc cgggtcgagc
      961 cccgtcgtcg gctcgtcgag gaagagcacg gtcggacggg ccacgagggc ggcggccaga
     1021 tcgagacgtc gtcgcatgcc gcctgagtac gtgcgggcgg gacgatccgc ggcgtccgtc
     1081 aggtcgaagc gctcgagcaa ctcgtcggcg cgtgatctcg cgaatgacgg agtctggtgg
     1141 gtgagtcgcc cgacgaggcg gaggttctca cgcccggtca ggttctcgtc gacggccgcg
     1201 tattggccag cgaggccgat cagctcccgc accgtctgag ggtgttggca gacgtccaag
     1261 ccgaggacgg tcgcttgtcc tccgtcgggc cgcagcacgg tggtgaggat gcggacggcg
     1321 gtcgtcttgc cggcgccgtt ggggccgagg aggccgagca cggcacccga gggcaccgcg
     1381 aagtcgactc cagacaacgc ctcgacggag ccgaagcgct tgatgaggcc ctcgacgcgg
     1441 atcgcgggat ccgcgccact gagactgggg ggagtcacgg tcatgacgac gatcatcgcg
     1501 ccaatagttg aagaagatca aggttcgatc tgttcaagtg ttgaattact tgaactaccg
     1561 cgccggcggg acgtaggatg gggctgtggt ggacgtgtcg gcgaagcggc gcccgagcag
     1621 ggcacgcgac gatctcgccg acgacgcctg gcagctgatg gccggcttct tcttcgagat
     1681 cggcatgcgc cacatgagcg agtcccggca ggccaccggt ctgaacccgg gagcgatcaa
     1741 agccctccag tcgctcgatc ccgaccagcc gaggacgatg ggatcgctgg cggaggcctg
     1801 gcactgcgac gcgtcgaacg tgacgtggct cgtggaccgg ctcgaagagc ggggtctggt
     1861 cgaacggcga acgctgccga ccgatcgacg ggtgaagacg gtggtcttga ccgactccgg
     1921 caaggcgctc cgcaacgacg tcgacgccat ctgggccaag gcgccggaac agttccgagc
     1981 cctcgaccgt gactccctca gcgggctcat cgaggtcatg cgcaagctcc gaccgagctg
     2041 agggcagttg cgggccccgg cgtagactcg tcgcgccgcg ggtgtagctc aatggtcaga
     2101 gttccagcct tccaagctgg ctatgcgggt tcgattcccg tcacccgctc tgc
//
LOCUS       DATBFZ010000182         4398 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_120894, whole genome shotgun sequence.
ACCESSION   DATBFZ010000182 DATBFZ010000000
VERSION     DATBFZ010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 4398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4398
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120894"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1574)
                     /locus_tag="VH419_05535"
     CDS             complement(<1..1574)
                     /locus_tag="VH419_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEY7043112.1"
                     /translation="MREYTTPATYTVPDTGNLTDDIVSNARDHADAVVFSRRTDRVWE
                     DVTAAEFLAEVQDLAKGLISAGVGEGDRVALLSKTRYEWTLADYAIWYVGAATVPIYE
                     TSSQDQIGWVLSDSEPTLAIVDTAENRANIDAVTAGLPSLKQVIGIDDGGLAELAQTG
                     RASDITDDDLERRRSAVTPDTVATLIYTSGTTGRPKGCTLTHGNFMFELKVAIGVTPE
                     LFEVEEASTLLFLPLAHVFARILQVGAVMARARLGHTADIKNLVNDLGTFQPTFILAV
                     PRVFEKVFNTASQRAISDGRGKIFGAAVSTAVAYSQALDRSGPGPLLRVRHRLFDRLV
                     YSKLRAAMGGRTEYAISGGAPLGDRLAHFFRGIGLQILEGYGLTETTAALAVNLPAAQ
                     RIGTVGRPLPGTSVRVADDGELLFRGGQVFTGYFNNPEATAEAFDDEGWFRTGDVGEI
                     DDDGYVRITGRKKEILVTAGGKNVAPAVLEDRIRAHHLVSQCMVVGDGKPFVAALVTI
                     DPESLPAWMEANGKTGS"
     gene            1855..2346
                     /locus_tag="VH419_05540"
     CDS             1855..2346
                     /locus_tag="VH419_05540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043113.1"
                     /translation="MAYIAPTSKRRDNPMLRNKKVAALVGAALIGGTALSGAAFVNTA
                     DASSTTHTLKFVAISDRDHRAGNNGFQGTEIERSHGHVVGYDVISGIFNERAHSVKIY
                     VAISRRGGLLFARLHSTSETTYVGTVTGGSGKFAGATGSLTAHNAPHNDDKTFVTIHY
                     TLP"
     gene            2529..2966
                     /locus_tag="VH419_05545"
     CDS             2529..2966
                     /locus_tag="VH419_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY7043114.1"
                     /translation="MADQTTSSILISADPAGVMAVIADFPSYPAWAKGVKSAEVLTEY
                     DDGRAEQVAFVLDAAPIRDEYTLAYDWDGDKQVTWSLVKAKMLKSMEGAYVLEPKGDD
                     TEVTYQLAVDLTIPMIGMLKRKGEKVIIDTALKGLKKRVETLS"
     gene            3021..4292
                     /locus_tag="VH419_05550"
     CDS             3021..4292
                     /locus_tag="VH419_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HEY7043115.1"
                     /translation="MRVILFTGKGGVGKTTAAAGTATLGALRGHRTLVLSTDAAHSLG
                     DAFDIPAGPDPNEVDDNLFVQQVDAQRRFERSWGEIQTYLMSVLDTVGVDPIAAEELT
                     VLPGAEEVLALLEVRDQARSGRWDLVVVDCAPTAETLRLLALPEALGWYMDRVLPMER
                     RMVKVLKPVLTRAAGVPMPRDSVFDAVEHLHADLAEVRSLLTGTDASVRLVLTPESVV
                     VAEARRSLTTLSLYGYRVDGVVANRIFPAADADAWRRQWVAAQAEMLAEVETSFAPLD
                     VWRSPYRSCEPVGLTELAAFAAELYGHDDPFARPHGQDPVDVRRSGSTIRLRVALPFA
                     DRHDVDLARRGDELVVTVGSYRRLLALPAALRRASVVGASMRDGALVVRFDTAGEGEP
                     LPVELPGSAEDDIDASRVGASGTAIGENAQP"
BASE COUNT          694 a         1485 c         1517 g          702 t
ORIGIN      
        1 gagccggtct tgccgttggc ctccatccag gccggcagcg actccgggtc gatggtgacc
       61 agcgcggcga cgaacggctt gccgtcgccg acgaccatgc actggctgac caggtggtgc
      121 gcgcggatcc ggtcctccag caccgccggc gcgacgttct tgccgccggc ggtgacgagg
      181 atctccttct tgcggccagt gatgcgcacg tagccgtcgt cgtcgatctc gcccacgtcg
      241 ccggtgcgga accagccctc gtcgtcgaac gcttcggcgg tcgcctccgg gttgttgaag
      301 tagccggtga agacctgccc accgcggaac agcagctctc cgtcatcggc gacccgcacg
      361 ctcgtgcccg gaagcggccg gccgaccgta ccgatgcgct gcgccgctgg gaggttgacg
      421 gccagtgccg ccgtcgtctc ggtcaggccg tacccctcga ggatctgcag cccgataccg
      481 cggaagaagt gcgcgagccg gtcaccgagt ggggcaccac ctgagatggc gtactccgtc
      541 cgaccgccca tggcggcgcg cagcttggag tacaccaacc ggtcgaacaa ccggtgtcga
      601 acccgcagca gcggaccggg accgctgcgg tccagcgcct ggctgtacgc caccgccgtg
      661 ctgacggcgg caccgaagat cttgccgcga ccgtcggaga tcgcccgctg actcgcggtg
      721 ttgaacacct tctcgaagac ccgcggaacc gccaggatga acgttggctg gaacgtgccg
      781 aggtcgttga ccaggttctt gatgtcggct gtgtgaccga gccgagcacg cgccatcacg
      841 gccccgacct ggaggatccg ggcgaacacg tgggccagcg gcaggaagag gagggtgctt
      901 gcttcctcga cctcgaacag ctcaggggtg acgccgatcg cgaccttcag ctcgaacatg
      961 aagttgccgt gcgtgagcgt gcaacccttc ggtctgccgg tcgtgcccga cgtgtagatc
     1021 agcgtggcga ccgtgtccgg cgtcaccgcc gaccggcggc gctcgaggtc gtcgtcagtg
     1081 atgtcggagg ctcgcccggt ctgcgcgagc tcggccagac ctccgtcgtc gatgccgatc
     1141 acctgcttca gtgacggcag tccggcagtg accgcgtcga tgttggcacg gttctccgca
     1201 gtgtcgacga tcgcgagggt gggctcggag tcggagagca cccaaccgat ctggtcctgc
     1261 gacgacgtct cgtagatcgg tacggtcgcc gcaccgacgt accagatcgc gtagtccgcg
     1321 agggtccact cgtaccgggt cttcgacaac agcgcgacgc ggtctccctc gccgacgccg
     1381 gccgagatca gccctttggc gagatcctgc acctcggcca ggaactcggc agcggtgacg
     1441 tcctcccaga ctctgtccgt acgccggctg aagacgacgg cgtcggcatg gtcgcgggcg
     1501 ttcgacacga tgtcgtcggt gaggttgccg gtgtccggca cggtgtaggt ggccggagtg
     1561 gtgtactcgc gcacgaaccc tcccgaagtc ggttttctac tcgggcaatc taccgtcggc
     1621 tcggtgcgag cgacttcatc gaggtccgcg ggggacgacc atcaaggccg agaggctccc
     1681 agcaactggc cgacgtgtga cccaggacat cggtgcttgc acccaggggc gtggttctgg
     1741 cactggggtc gatatcgcca tagtcactcg atgacccata caggccatga tcgatgaata
     1801 tttcccacta catcgggggg tttcgcccgg cctcgagcag gacgacgatc gcggatggcg
     1861 tacatcgcgc ccacgtcgaa gcgaagggac aaccccatgc tccgtaacaa gaaggtcgcc
     1921 gcactcgtcg gcgccgccct gatcggcggc acagcgctct ccggtgccgc gttcgtgaac
     1981 accgccgacg ccagctcgac cacccacacg ctgaagttcg tcgccatctc cgatcgcgac
     2041 caccgggccg gaaacaacgg cttccagggc accgagatcg agcggagcca cggccacgtc
     2101 gtcggctacg acgtgatctc cgggatcttc aacgagcgtg cgcacagcgt caagatctac
     2161 gtcgcgatct cgcgtcgtgg aggcctcttg ttcgcccgcc tgcacagcac gtcggagacc
     2221 acgtacgtgg ggacggtcac cggcggctcc ggcaagttcg ccggtgcgac tggatcactg
     2281 accgcgcaca acgcgccaca caacgacgac aagacgttcg tgaccatcca ctacaccctg
     2341 ccgtaacggc gacagcgtcc cgcggagtgc ggagcggcca gtcgccggct tcgcgaagga
     2401 gcccgaccgg gtcctcggtg gcctgcactc cgcgggcatc accccaggag ctcgtcaggc
     2461 cgagcgcggg ccctagtagt ctcgccaccg ttcgatacgg ttgccgcgcc gacgaagggg
     2521 agccgcagat ggcagaccag acgacgtcga gcatcctcat ctctgccgac ccggccggcg
     2581 tgatggcggt gatcgcggac ttcccgtcat acccggcgtg ggcgaagggc gtgaagtcag
     2641 ccgaggtgct gacggagtac gacgacgggc gcgcggagca ggtcgcgttc gtgctcgacg
     2701 ccgcgccgat ccgcgacgag tacacgttgg cctacgactg ggacggcgac aagcaggtca
     2761 cctggtccct ggtgaaggcg aagatgctga agtcgatgga gggcgcgtac gtgctcgagc
     2821 ccaagggcga cgacaccgag gtgacgtacc agctggccgt cgacctcacc atcccgatga
     2881 tcgggatgct caagcggaag ggcgagaagg tcatcatcga caccgccctg aagggcttga
     2941 agaaacgcgt cgagaccttg tcctgaccgt cctgaccgtc ctggccgtcg tctgccgccg
     3001 acctcgaatc gagcctgatc gtgcgcgtca tcctcttcac cggcaagggc ggtgtcggaa
     3061 agaccaccgc agccgccggc accgcaacgc tcggagccct gcgcggccat cggacgctgg
     3121 tgctgagtac cgacgcggcg cactcgctgg gcgacgcgtt cgacatcccg gccggccccg
     3181 acccgaacga ggtcgacgac aacctgttcg tgcagcaggt cgacgcgcaa cggcggttcg
     3241 agcgctcgtg gggcgagatc cagacgtatc tgatgtcggt gctggacacc gtcggggtcg
     3301 acccgatcgc agccgaggag ctcacggtcc tgccgggtgc cgaggaggtc ctcgccctgc
     3361 tcgaggtccg cgaccaggcg cggtccggtc gctgggacct cgtcgtcgtg gactgtgcgc
     3421 cgaccgccga gacgttgcgg ctgctcgccc tgccggaggc gttgggctgg tacatggacc
     3481 gggtgctgcc gatggagcgc cggatggtga aggtgctgaa gccggtgctg acgcgtgccg
     3541 ccggagtccc gatgccgcgc gactcggtgt tcgacgctgt cgagcatctc cacgccgatc
     3601 tcgctgaggt ccggtccctt ctcacgggca ccgacgcgtc cgtccggctc gtgctgactc
     3661 ccgagtccgt cgtcgtcgcc gaggcccggc gctcgctgac gacgctgtcc ctctacggct
     3721 accgggtcga cggggtcgtg gcgaaccgga tcttccccgc ggccgatgcc gacgcgtggc
     3781 gccgtcagtg ggtggcggcg caggcagaga tgcttgccga ggtcgagacc tcttttgctc
     3841 cgttggacgt gtggcggtcg ccgtaccgct cctgcgagcc ggtcgggctc accgagctcg
     3901 ccgcgttcgc cgcggagctc tacggacacg acgacccgtt cgcccgaccg cacgggcagg
     3961 acccggtcga cgtacgccgt tcggggtcga cgatccggct ccgcgtcgca ctgccgttcg
     4021 ccgaccggca cgacgtcgac ctggcgcggc gaggtgacga gctcgtcgtc acggttggct
     4081 cgtaccgccg gttgctggcg ctgcccgccg cgttgcgcag ggcatctgtc gtcggggcgt
     4141 cgatgcggga cggcgctctg gtcgtgcggt tcgacacggc tggagagggg gagccgttgc
     4201 ctgtggagct gccggggtca gccgaagacg acatcgacgc cagccgggtg ggggcgtccg
     4261 ggacggcgat cggcgagaat gctcagccat gagcacagat tccgtcggat cgctcgctga
     4321 ggaggccgcg aagcttctcg ccgtcatcca ggggtgggcg ggtgaccaca gcggtcaggc
     4381 cggcgacacc gcgaccgg
//
LOCUS       DATBFZ010000183         3167 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_3402681, whole genome shotgun sequence.
ACCESSION   DATBFZ010000183 DATBFZ010000000
VERSION     DATBFZ010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 3167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3167
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3402681"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..498)
                     /locus_tag="VH419_05555"
     CDS             complement(<1..498)
                     /locus_tag="VH419_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epimerase"
                     /protein_id="HEY7043116.1"
                     /translation="MKLLVLGGSVFLSKAVAAEGVRRGHAVTCACRGSSGPVPEGADL
                     VEWDRTRPVPSGLAGAAYDAVVDVARQPSWVRRAVTAFPSAHWVFVSTINVYADDATP
                     GGHPGALPLRNPIEDDVDLSEDPESYGGMKVACEEFVREEAASALVVRPGLIVGPEDP
                     TGRFSY"
     gene            795..1157
                     /locus_tag="VH419_05560"
     CDS             795..1157
                     /locus_tag="VH419_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043117.1"
                     /translation="MLNQLHQPKGAVGIFSVVATLILALWGAFVIVAMLLWDDEEMTR
                     GDEVMLVVFGAIMIVGAIGFVIMARRPWLGATLAVVGSIALGVILFWTIVPMILGATF
                     IIVALVRAQAFASHNVPA"
     gene            1415..1915
                     /locus_tag="VH419_05565"
     CDS             1415..1915
                     /locus_tag="VH419_05565"
                     /inference="COORDINATES: protein motif:HMM:NF019886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY7043118.1"
                     /translation="MSVAAGDDAAFSELYAATFARIAAITTVIMQDRASGVEIAQDAF
                     VQLYVHWKKVGHYDAPEAWVRRVAIRLAMREAHRDSRRRQLSIAAWRPEDDDVSLPDV
                     DVLRAVASLPAIHRAVVALFYFEDLSVAEIARVLDKSEGSVKVALHRARRSLAAMLGE
                     EDYPHG"
     gene            1908..2555
                     /locus_tag="VH419_05570"
     CDS             1908..2555
                     /locus_tag="VH419_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043119.1"
                     /translation="MVDREPSDDLVAAALRRVVGEPTGDLGEVLARGTRVRRARTRRS
                     VVLLVAAVLAVAVALSFAVLRAPSTAPEPAHVPDHSQPSGISTGTYSTAPLSATTLIR
                     NGMKPTEVWAQQTVLTLRFERLTHDQTERNQNGSYTQFDAEDGGPPKKGDTGAFAVHG
                     RTITFVSSYHFQRATYIVHPTSDGFRLEVKHFYFKDPDERRIATAIWTTAAFHRN"
     gene            2822..>3167
                     /locus_tag="VH419_05575"
     CDS             2822..>3167
                     /locus_tag="VH419_05575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043120.1"
                     /translation="MTALLVAALFGFSAESASGASASDGSSSARSGPLRMVEMGDSWI
                     AGDHCDCVTFAGLWAADLQQQTGRAVQLTDLTGVHERSPVESKTSGSMLWALRHDRQT
                     RHILATSDIVLVS"
BASE COUNT          503 a         1107 c         1061 g          496 t
ORIGIN      
        1 gtagctgaac cggcccgtcg ggtcctccgg accgacgatc agacccggcc gcaccaccag
       61 cgccgacgcc gcctcctcgc gcacgaactc ctcgcacgcg accttcatcc cgccgtagga
      121 ctccgggtcc tcggagaggt cgacgtcgtc ctcgatcgga ttgcgcagcg gcagtgcccc
      181 cgggtggccg ccgggtgtcg catcgtcggc gtacacgttg atcgtcgaca cgaacaccca
      241 gtgggcgctg ggaaacgccg tcaccgcgcg tcgcacccac gacggctgcc gggcgacgtc
      301 gaccaccgcg tcgtacgccg cgccggcgag cccgctcgga accggtcgcg tgcggtccca
      361 ctcgaccagg tcggccccct ccggcaccgg tcccgagctg ccgcggcacg cgcacgtcac
      421 cgcatggccg cgacgcacgc cctccgccgc caccgccttg gagaggaaca ccgacccgcc
      481 cagcaccagg agcttcatcc ctccaccgaa cactccggtg gtcgagcttg tcgagaccgc
      541 ccgacgggca acctcgaatc ggacgcggct tggacgtcat acgcttcggc gcctatcgcc
      601 atcggttcgt acgacgtccc gagtgctagg gatttccccg attcctttct gcgtcgcggg
      661 acgcaggctg gaccttccat gtcccgccag gtgcgcaaag caggcagatc cgtgctggcc
      721 cgtgggtgtc tcacggcggg cgtccaccag tcctgacgtg aatcgccagg aacgagcccg
      781 aggaagtgat catcatgctc aaccagctgc atcagcccaa gggagcggtc gggatcttca
      841 gcgtcgtcgc gaccctgatc ctcgccctct ggggagcctt cgtgatcgtg gcgatgctgc
      901 tctgggatga cgaggagatg acccgaggcg acgaggtgat gctcgtcgtg ttcggcgcga
      961 tcatgatcgt gggagccatc gggttcgtca tcatggccag gaggccatgg ctgggagcca
     1021 ccttggccgt cgtgggcagc atcgcgctcg gggtcatcct cttctggacg atcgtgccca
     1081 tgattctcgg cgccactttc atcatcgtcg ccctcgtgcg cgcgcaggcg ttcgccagtc
     1141 acaacgtccc cgcgtaggcg cgaaccgtcc acgcgtgggc ccgccgaacc ggaccgcgac
     1201 ccgaacgtcc gggcgtgagc ggacgcccgc gcgcctgccg cgcgaccgtc cggcagtacg
     1261 acacgccggg gcgagatcac acgggcaggt ggccacccgg aaccgtggcc gtggcggtaa
     1321 ccagacaccg cggtcgagac tgtctgttga tgtggtctcc aggcttcgga cgcttcgcgc
     1381 agatggcggc gcggcggtgc ccaccatggg gaaggtgagc gtggcggcgg gcgacgacgc
     1441 tgccttctcc gagctctatg cggccacctt cgcgcggatc gcagcgatca cgaccgtgat
     1501 catgcaagac agggcctcgg gtgtggagat cgcccaggac gcgttcgtcc agctgtacgt
     1561 gcactggaag aaggtcggcc actacgacgc cccggaggct tgggtgcggc gggtggcgat
     1621 ccggctcgcg atgcgggagg cacatcgcga ctctcggcgg cgacagctga gcatcgccgc
     1681 ctggcggccc gaggacgacg acgtctcctt gcccgacgtg gatgtgctgc gagccgtcgc
     1741 gagtcttccg gcgatccacc gggccgtggt ggcgctgttc tacttcgagg acctgtccgt
     1801 ggcagagatc gcacgagtgt tggacaagtc ggagggatcg gtgaaggtcg cgctgcacag
     1861 ggctcgccgc tcactcgccg cgatgttggg agaggaggac tatccacatg gttgaccgcg
     1921 agccgtcgga tgacctggtc gcggccgctc tccgccgggt ggtgggcgag ccgacgggtg
     1981 acctcggcga ggtcctggcg cggggcacga gggtacgccg ggcgcgtacc cggcgatccg
     2041 tcgtcctctt ggtggctgcc gtgctcgctg tagccgtcgc gctgtcgttc gccgtcctgc
     2101 gtgcgccgtc gaccgcgccg gagcccgcac acgtgcccga tcactcccag ccgagcggca
     2161 tctcgaccgg cacgtactcg acagctccgc tgtcggccac gacgctgatc cgcaatggga
     2221 tgaagccaac ggaggtctgg gcgcagcaga cggtgctgac cttgaggttc gagcggctca
     2281 cacacgacca gacggaacgc aaccagaacg gctcgtacac gcagttcgac gccgaggacg
     2341 gcgggccacc caagaagggt gacaccggcg ccttcgcggt ccacggtcgc acgatcacct
     2401 tcgtcagcag ctatcacttc cagcgagcga cgtacatcgt gcatcccacg tccgacgggt
     2461 tccggctcga ggtcaagcac ttctacttca aggatccgga cgagcgacgg atcgccaccg
     2521 ccatctggac caccgctgcg ttccaccgca actgagcccg accgaccgtc ggcaccgaag
     2581 ccgatcgtcg gagtaaccac gcagacgcat ccggcctgtt tctcgtgtga accggcagtc
     2641 gctcgacgtg gcggtctccg cctcgaccag ctgctggcgg ctcccagacc ggtgtcctca
     2701 cggtcaccga gcaactcgaa cggaaggcag accgatgaac gagctccatg gacagcctgc
     2761 cgcacgcaca cgacggacgt cgcggtcgct ggtgaggctg agacgaggtg gtgcgctcct
     2821 catgacggcg ctcctggtcg cggctctctt cggcttcagc gccgaatctg catccggggc
     2881 gtccgcatcc gacgggtcgt cgagcgccag gtcggggccg ctgcgcatgg tcgagatggg
     2941 tgactcgtgg atcgccggcg accactgcga ctgcgtgacg ttcgccgggc tgtgggcagc
     3001 ggacctccag cagcagacgg gccgcgcggt gcagctgacc gacctgaccg gcgttcacga
     3061 acgcagcccg gtcgagtcca agacgtccgg cagcatgctc tgggccctgc gccacgacag
     3121 gcagacccga cacatcctcg ccacctcgga catcgtgctg gtgtcaa
//
LOCUS       DATBFZ010000184         3545 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_5969665, whole genome shotgun sequence.
ACCESSION   DATBFZ010000184 DATBFZ010000000
VERSION     DATBFZ010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 3545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3545
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5969665"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            146..892
                     /locus_tag="VH419_05580"
     CDS             146..892
                     /locus_tag="VH419_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020388819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY7043121.1"
                     /translation="MYDAAFRRTCLQELEQGSSLSTVSRRHGVTRSTLREWRDNPENV
                     PPIQCPQCQGAPLAADSYSHLLGLYLGDGCLSACRRGVYMLRISCDARYRRLVAEAVE
                     SISLVRPGVSVFKVTAPGVTVVQAYWKHWPCLFPQHGPGRKHERPIVLEDWQVRAVDE
                     HPGPFLRGLFHSDGCRVTNWTVHPLRAGPKRYEYPRYFFSNQSADIIGICTRTLDRLG
                     IAWTMPRYNTVSVARRASVTLMDELIGPKS"
     gene            complement(908..1174)
                     /locus_tag="VH419_05585"
     CDS             complement(908..1174)
                     /locus_tag="VH419_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043122.1"
                     /translation="MTTSPQEPLKSARPRSADTSEEAQNERDERLVEQTPELSEPGGY
                     DDRQESLKESDQDLFERKQHDPDASYKDEDLPKSEPRKQPRQQP"
     gene            complement(1232..2470)
                     /locus_tag="VH419_05590"
     CDS             complement(1232..2470)
                     /locus_tag="VH419_05590"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HEY7043123.1"
                     /translation="MVKRYGGRAARGAGVAAVCLSLVVACSTTTSEVSQRTTVDRSDR
                     VASWTAGAASLGRWSPGRVSRSARSPDRIERRIAAMTLRDKIRQLLVLAYGGTRAPTD
                     LIRRFHPGGLISFSDNLVSDGQIRAMNSRSQRVARHTGQPLLLMTDQEGGPVTRLPGT
                     QGVPGGAEFHGNAPWARRTALHTGRTMRSLGLNVDLAPVADVNTVGHRGVIGDRSFSS
                     HPYVAARLVRAQVCGYHQGRVAATAKHFPGHGSTTTDSHLRPARIRESKRVWRHTDLP
                     PFARAVRAGVDMIMVGHLSFPAMDRTGRPATISPLLDKRLLRHDLGFRGVVITDALTM
                     RGITAYGSAGSIAVRAIRSGVDLLLMTPRPALAVRGIAAAVHQGKLGVGRINASVRRI
                     LVLKQRLGLYRASEHLERCR"
     gene            complement(2476..>3545)
                     /gene="pyk"
                     /locus_tag="VH419_05595"
     CDS             complement(2476..>3545)
                     /gene="pyk"
                     /locus_tag="VH419_05595"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020388818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEY7043124.1"
                     /translation="ATEVGDTDVRATVEVGGRVSDGKGINLPGVAVSVPALSEKDVED
                     LRWALHLRTDMVALSFVRAAQDVVDVRRIMDEEREWVPVIAKIEKPQAIDQIEEIITA
                     FDGVMVARGDLGVEMPLEVVPFLQKRVVELARRNAKPVVVATQMLESMISSPRPTRAE
                     ASDVANAVLDGADAVMLSGETSVGAYPIESVRTMARIIESTEDHGLARMAAIDWQPHT
                     RGGIIAKAAAEVAERVGAKFLVAFSQSGDTARRLARYRSAVPVLAFTPEPVVRSQLAM
                     TWGIETFLFERVEHTDEMVMQVDGALLDIGRCDIGEQVVIVAGSPPGIPGSTNALRIH
                     RMGDAINGVAPAYRSQARTHG"
BASE COUNT          533 a         1230 c         1195 g          587 t
ORIGIN      
        1 caaccggcag agaaggcgga ttcaaaaccc gtacagtgtg ggttcgactc ccacccaggg
       61 cacctctcaa cagcgccgga tggcgacatc cacagcgaag gtcagtggtc cgaccagcgt
      121 cccctgagcg cgtcagtctg ggcgaatgta tgacgcggcg tttcggcgaa cctgcctgca
      181 ggagctcgag cagggctcgt cactgagcac ggtgagccgg cgccacggtg tcacccgatc
      241 gacactgcgc gagtggcggg acaacccgga aaacgtcccg ccgatccaat gtccgcagtg
      301 ccagggcgca ccgctggcgg ctgacagcta ctcccatctt ctcggcctct acctgggtga
      361 cggatgtctc agcgcctgtc gccgaggtgt gtacatgctc cgcatctcat gcgatgcgag
      421 ataccgccgg ctcgtcgcgg aagcggtgga gagcatctcg ctcgtccgtc ctggtgtcag
      481 cgtcttcaag gtgaccgcgc cgggagtcac tgtcgtgcag gcgtactgga aacactggcc
      541 gtgtctgttt cctcaacacg gccccgggcg caagcatgag cggcccatcg tgctggagga
      601 ctggcaggtc cgggccgtgg acgagcatcc cggaccgttt ctccgcggtc tgttccactc
      661 cgacggctgc cgggtgacga actggaccgt gcacccgctc cgggccgggc cgaaacgcta
      721 tgagtatccg cggtacttct tctccaacca gtccgccgac atcatcggca tctgcacgcg
      781 caccctcgac cggctgggca tcgcctggac gatgccgcgg tacaacaccg tctcggtcgc
      841 ccgccgagca tcggtcacgc tcatggacga gctgatcgga ccgaagagct gaccggctgt
      901 cctcgcgtca gggctgctgg cgcggctgct tgcgcggctc ggacttcggg aggtcctcgt
      961 ccttgtacga agcgtccgga tcgtgctgct tgcgctcgaa caggtcctgg tcggactcct
     1021 tcaggctctc ctgacggtcg tcgtacccgc cgggctcgga cagctccggt gtctgctcga
     1081 ccagccgttc gtcgcgctcg ttctgtgcct cttcggatgt gtcggcggag cgcgggcggg
     1141 ccgacttcaa cggttcctgt ggtgatgtgg tcatgatctg acgtcgtacc cggtcggcga
     1201 gcgctctaac ctggccacac cagagacgct gtcagcgaca gcgttccaag tgctccgagg
     1261 cccggtagag cccgagccgt tgtttgagaa cgaggatgcg gcgtacggac gcgttgatcc
     1321 ggccgacgcc gagcttgccc tggtggacgg ccgccgcgat cccgcggacc gccagggccg
     1381 gtcgcggtgt catcagcagc aggtcgacgc cggacctgat ggcgcgcacc gcgatggatc
     1441 ccgcgctccc gtatgccgtg atgccccgca tcgtcagcgc gtcggtgatg acgacaccgc
     1501 ggaagccgag gtcgtggcga agcaaccgct tgtcgagaag gggcgagatc gtggccggtc
     1561 ggccggtccg gtccatcgcg gggaacgaca ggtgcccgac catgatcatg tcgacgcccg
     1621 cgcgaaccgc ccgggcgaag ggtggcaggt cggtgtgccg ccagacccgc ttgctctccc
     1681 ggatccgggc cggccgcagg tggctgtccg tcgtcgtgga tccgtgtccg gggaagtgct
     1741 tcgccgtggc agcgacacga ccctggtggt agccgcagac ttgcgcccgc accagccggg
     1801 cggcgacgta cgggtgcgat gagaacgacc ggtccccgat gacgccgcga tggccgacgg
     1861 tgttgacgtc agcgacgggg gccaggtcga cgttgagtcc cagcgaccgc atcgtccgcc
     1921 cggtgtggag tgccgtccgt cgagcccacg gggcgttgcc gtggaactcc gcgccaccgg
     1981 gtacaccctg tgtgcccggc agcctggtga ccgggccgcc ctcctggtcg gtcatgagca
     2041 gcagcggttg acccgtgtgc cgcgccacgc gctgcgagcg gctgttcatc gcgcgaatct
     2101 gtccatcgct caccaggttg tcggagaagg agatcagacc gccggggtga aaacgccgga
     2161 tgaggtcggt gggagcacgg gtcccgccgt aggcgagcac gaggagctgc cggatcttgt
     2221 cccgcagcgt catcgcggcg atccgtcgct cgatccgatc cgggctccgg gcggaccgcg
     2281 agacgcggcc agggctccag cgtccgagcg acgcggcgcc cgccgtccac gacgccaccc
     2341 ggtcggaccg gtcgacagtg gtgcgctgcg acacctcgct cgtcgtggtg ctgcacgcaa
     2401 cgacgagact caggcacact gcggctacgc cggccccacg cgccgccctt ccgccgtagc
     2461 gtttcaccat tcccttcacc cgtgggtccg tgcctgcgat cgatacgccg gcgccacccc
     2521 gttgatcgcg tcgcccatcc ggtggatacg cagcgcgttc gtcgagcccg ggattccggg
     2581 cggactgccc gcgacgatga cgacctgctc tccgatgtca caccgcccga tgtcgagcag
     2641 tgccccgtcg acctgcatga ccatctcgtc ggtgtgctcg acccgttcga agaggaacgt
     2701 ctcgatgccc cacgtcatgg ccagctgcga gcggacgacg ggctcgggtg tgaaagccag
     2761 caccgggacc gccgagcggt accgggcgag ccgccgagcg gtgtcaccgg actggctgaa
     2821 cgcgacgagg aacttggcac cgacccgttc ggccacctcg gcagccgcct tggcgatgat
     2881 cccgcctcgt gtgtgcggtt gccagtcgat cgcggccatc cgggcgaggc cgtggtcctc
     2941 ggtcgactcg atgatccgtg ccatcgtccg cacggactcg atcgggtacg cgccgacgct
     3001 cgtctccccg gacagcatca cggcgtcggc accgtcgagc acggcgttgg caacgtcgga
     3061 ggcctccgcg cgggtcggcc gtggtgacga gatcatcgac tcgagcatct gggtggcgac
     3121 gacgaccggc ttggcgttgc gacgagcgag ctcgacgacc cgcttctgga ggaacgggac
     3181 gacctcgagt ggcatctcga cgcccaggtc accgcgcgcc accatcaccc cgtcgaacgc
     3241 ggtgatgatc tcctcgatct ggtcgatcgc ctgcggtttc tcgatcttcg cgatcacggg
     3301 cacccactcg cgctcctcgt ccatgatgcg gcgtacgtcg acgacgtcct gggcggcgcg
     3361 cacgaacgac agcgcgacca tgtctgtgcg caggtgcagc gcccagcgaa ggtcctcgac
     3421 gtccttctcg ctgagcgcgg ggacgctgac cgccacaccg ggcaggttga tccccttgcc
     3481 gtcactaacg cggccgccca cctccacggt tgccctgacg tccgtgtcgc ccacctcggt
     3541 ggccc
//
LOCUS       DATBFZ010000185         1513 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_452294, whole genome shotgun sequence.
ACCESSION   DATBFZ010000185 DATBFZ010000000
VERSION     DATBFZ010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_452294"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="VH419_05600"
     CDS             complement(<1..317)
                     /locus_tag="VH419_05600"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEY7043125.1"
                     /translation="MSLLRVRGHGRHIVAVVLSIATLAAATPPTGAVRDAARWSWPLQ
                     PDHSVVRGFEPPADPWGPGHRGVDIAGAPGQPVLAVAAGTVTFAGQVAGTGVVVVNHG
                     RIRS"
     gene            677..>1513
                     /gene="rpsB"
                     /locus_tag="VH419_05605"
     CDS             677..>1513
                     /gene="rpsB"
                     /locus_tag="VH419_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020391164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEY7043126.1"
                     /translation="MAVVTMRQLLESGVHFGHQTRRWNPKMKRFIMTERNGIYIIDLQ
                     QSLAYIDRSYAFIKGAVASGGNVLFVGTKKQAQEAISEQAKRVGMPYVNQRWLGGMLT
                     NFQTVHQRLQRLKELEEVNFDDAASTSRTKKELLQMKREKDKLERTLGGIRDMARVPA
                     AVWIVDTNKEHLAVDEARKLGIPIIAILDSNCDPDVVDYPIPGNDDAIRSVTLLTRVV
                     ADAVADGLMARAGVKSGQEATGEELGAEEPLAEWERALLQGEADTASPAAAPAVTDAP
                     AAA"
BASE COUNT          282 a          537 c          485 g          209 t
ORIGIN      
        1 gaccggatcc gcccatggtt gaccaccacg actccggtgc ccgccacctg acccgcgaag
       61 gtcaccgttc ccgctgccac ggcgagcacc ggctggccgg gagcgccggc gatgtcgacg
      121 ccgcggtgac cgggacccca cgggtccgca ggcggttcga acccgcgcac cacggagtgg
      181 tctggttgca acggccacga ccagcgtgct gcgtcgcgaa cagcaccggt tggcggcgtc
      241 gctgcggcga gtgtcgcgat cgacagcacc accgcgacga tgtgccggcc gtggccacga
      301 accctcagca atgacatgca tccaccgtgg ccgacagacc ctcacctgcc gccccgttgc
      361 cagcaccagt ggacgagtgc cacgcgccac cgagcctgtg ggcaccccgc aaatgcggga
      421 agttccgcgg tttgaccgcc ggaaatcccg gaacttcccg caattcgcgg gagcccgaca
      481 cgatccacta gactgacccg gcgacccgtc cgtgcgggtc gacttcgcac gcccacttct
      541 cgcagtccgc tgcgagcgtg tgtcgtggtc tccttcggga gtggcacgcg cgtggacgtc
      601 agggtttcga ccgttcgccg gcctacagag gtcaacgcag tcgagacgac caaccgcaaa
      661 caggaggaca tcgcacatgg ccgtcgtgac catgcgccag ctgctcgaga gcggcgtcca
      721 cttcgggcac cagacccgtc gatggaaccc caagatgaag cgattcatca tgaccgagcg
      781 caacggcatc tacatcatcg acctgcagca atcactggcg tacatcgatc gcagctacgc
      841 cttcatcaag ggtgcggtcg cgtccggcgg caacgtgctc ttcgtcggga ccaagaagca
      901 ggcgcaggag gccatctccg agcaggccaa gcgcgtcggc atgccgtacg tcaaccagcg
      961 ctggctgggt ggcatgctca ccaacttcca gaccgtgcac cagcgcctgc agcgcctcaa
     1021 ggagctcgag gaggtcaact tcgacgacgc ggcgagcacc agccgcacga agaaggagct
     1081 cctgcagatg aagcgggaga aggacaagct cgagcgcacc ctcggcggga tccgtgacat
     1141 ggcccgggtg ccggccgccg tgtggatcgt cgacaccaac aaggagcacc tcgctgtcga
     1201 cgaggctcgc aagctcggta tcccgatcat cgccatcctc gacagcaact gcgaccccga
     1261 cgtcgtcgac tatccgatcc ccggcaacga cgacgcgatc cgctccgtca cattgctgac
     1321 ccgggtcgtg gcggacgctg ttgcggatgg cctcatggcg cgtgccggcg tcaagagcgg
     1381 tcaggaggcg acgggtgagg agctcggcgc cgaggaaccg ctcgccgagt gggagcgcgc
     1441 cctgctgcag ggtgaggccg acaccgccag ccctgccgcc gccccagcag tgactgatgc
     1501 acccgccgcg gcc
//
LOCUS       DATBFZ010000186        18050 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_2092709, whole genome shotgun sequence.
ACCESSION   DATBFZ010000186 DATBFZ010000000
VERSION     DATBFZ010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 18050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18050
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2092709"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..524)
                     /locus_tag="VH419_05610"
     CDS             complement(54..524)
                     /locus_tag="VH419_05610"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY7043127.1"
                     /translation="MSPRIRVAGIRDAAEIARLRRAWVEEQAGSPIEDEDYDAEFAAW
                     FEREAHQRVTWLAEIGDCIIGMLNMLVFTRMPKPRPVGVPQHPRQWGYIANVYVEQAD
                     RDAGVGKLLLDAALEYADEHGYVRVVLSPSERSIPFYRRAGFVPASSLLIRPHP"
     gene            complement(521..919)
                     /locus_tag="VH419_05615"
     CDS             complement(521..919)
                     /locus_tag="VH419_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020580084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY7043128.1"
                     /translation="MTTPEYAGAAHPRQLVETFVSSLEARDWDRWTATMHADVVYELP
                     QSRERIRGRDTYLQFNQEYPGDWHLRLKVLIADEQHGVAWFGWRVGDDREQDAMAFFG
                     FDDGLISQVTDFWPEPYEPPAGREHLVERW"
     gene            1081..1725
                     /locus_tag="VH419_05620"
     CDS             1081..1725
                     /locus_tag="VH419_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043129.1"
                     /translation="MTGYTVVHLDEIEEFADAGCHYRPIRHHLGITAFGVIAWSARAA
                     GDRVINEHDEDDPTADQELFLVLRGHALFELDGDRVDAPAGTLLFAPPGTKRMASATE
                     AGTTVLALEGTPGRAYEARGWELWAPLAPLHAAGEYAEVADRLGALVAANPQYPMLFF
                     NLACCESLCGRTREALDHLKHAIEMSEEFRQSARDDSDLDPIRDEPDFKQLVSG"
     gene            complement(1896..2642)
                     /locus_tag="VH419_05625"
     CDS             complement(1896..2642)
                     /locus_tag="VH419_05625"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY7043130.1"
                     /translation="MDETRVVRATSFGGWADEYQDCRPSYPDSAVNWLLGDAREVVEV
                     GAGTGKLTDRLVEHKDVSLRVTEIDGRMLRLVSERHSALPVHEAAVTNLPVEDGSVDA
                     VLVADAWHWFPNAEAAAEVGRVLRRGGWLGCIWNDLSAATPDWQWEAVNLSADIAARV
                     RALPPLDRLGLTTGDAEQEVVRWNWSLTPREWRGYVSTVSHVRTMPPGERLEVLDAAE
                     RLASAACAATGASTVSLILDAVCIRWYPAQ"
     gene            complement(2857..4788)
                     /locus_tag="VH419_05630"
     CDS             complement(2857..4788)
                     /locus_tag="VH419_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012919943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HEY7043131.1"
                     /translation="MPVESVFAQLEPLLPTVQKPIQYVGGELNSTAKDWDAVSVRWAL
                     MYPDAYEVGLPNQGVQILYEILNERDWILAERTYAAFADMEQVMREHGIPQFTVDAHR
                     PVGAFDVLGISFATELGYTNMLTALDLAGIPLHAADRDDAHPIVIAGGHSAFNPEPVA
                     DFLDAAVLGDGEEIVLAISDVVREWKDEGRPGGRDELLMRLARSGGVYVPRFYDVDYL
                     PDGRIKRVVPNRAGVPWRVHKHTLMDLDQWPYPRRPLVPLAETVHERYSVEIFRGCTR
                     GCRFCQAGMITRPVRERSITTIGDMVQNGLEKSGFEEVGLLSLSSADHSEIGEVAKGL
                     ADRYDGTNVSLSLPSTRVDAFNVTLANELSRNGRRSGLTFAPEGGSERLRKVINKMVS
                     EEDLIRTVATAYSHGWRQVKLYFMCGLPTETDADVLEIAELAKKVIATGREASGRRDI
                     RCTVSIGGFVPKPHTPFQWASQLDHETTDERLRKLRETVRADKQYGRAIGFRYHDGKP
                     GIVEGLLSRGDRRVGRIIEEAWRDGGRFDGWSEHFSYDRWAAASERALVDEPVDLDWY
                     TLREREYSEVLPWDHLDSGLDRDWLWEDWQDALNPAGAVEVDDCRWTPCFDCGVCPEM
                     GTEIQMGPTGQRLLPLSVV"
     gene            complement(4885..5670)
                     /locus_tag="VH419_05635"
     CDS             complement(4885..5670)
                     /locus_tag="VH419_05635"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEY7043132.1"
                     /translation="MFQASATPTAHACHVPPFSPIAAPRSYADSDSAYDGRGQAMAGT
                     RIGVLGTGMVGQTLAKRLADVGYDVMVGARSRDSDSLAAFAETDRLATGSFEDAAAFG
                     EIVLNATNGSHALEALSMAGAENLAGKPVIDVTNDLEPVEGGYPKPRATADSSLGQTL
                     QDAFPDAHVVKTLNTMNCQVMADPSIVEGDHVVFMSGNDDAAKRTVSAMLADFGWRPQ
                     QIVDLGGIETAAAPEMMMSAWMRVRIARGMDAPPFNWAIHSGT"
     gene            complement(5778..7013)
                     /gene="rodA"
                     /locus_tag="VH419_05640"
     CDS             complement(5778..7013)
                     /gene="rodA"
                     /locus_tag="VH419_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012919935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HEY7043133.1"
                     /translation="MVMTAHVATTRTSRRVNVTERRSAWLRLDWVVVLATAALLTMGS
                     LLVWSATANNDALTGGDSTAYLTRHLANVAVGLVLAFFVAATDHRWVRIWAPLVYLAA
                     IVGLVLVLSPVGAVINGSRSWIHVGGMSIQPAEFAKLGVVAGLALLLAEKTEHRKGLS
                     QLRDRDVVGALALVVVPAVLILAQPDLGTLLVLGVTVFGLVAVAGAPKRWLVLLAGGA
                     VAGAVAAVQLGLLKGYQLDRFGSFLDPTLDPRGAGYNTTQARIAIGNGGLFGQGLFQG
                     SQTQSGFVPEQHTDFVFTVAGEELGLVGAGALIALYLVLLWRAVRIATRADDMFGRLA
                     GAGIVCWFGFQAFQNVGMCLGIMPVTGVPLPMVSYGGTSMFASLMAIGLLLNIHLRTE
                     RHLGLGVVLRGRIARSRRI"
     gene            complement(7007..9175)
                     /gene="mrdA"
                     /locus_tag="VH419_05645"
     CDS             complement(7007..9175)
                     /gene="mrdA"
                     /locus_tag="VH419_05645"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017934160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEY7043134.1"
                     /translation="MLKTRSRLRLVVVQAMVLSLFVTLFARLWYIQVVAGADYQAAAQ
                     DNTIRDIDIPAPRGLIVDAMGRPLVANRTSWVVTVDRDVLAKLGDSTRNAVLSRLADT
                     LGLSRVELERRTKTCGEAGAAKPPICWNGSPYEPVPVAEDVTQRLAASILEQNEDYPG
                     ITAEARKVRAYPSPYGINAAHVLGYISPITEQELAKAEQNGHNLSPLSLTGRAGLEAG
                     YDRYLRGHDGVREVSVDSMGRVLGYDGMTAPRPGDTLVTSIDAKVQAVAERELVRSIM
                     VARQTTDPVTHRKYVADSGSVVVLDAKTGRVVALASYPTYDPKVWVGGITQGDLERLY
                     SPKADTPLLSRPMQGLFAPGSTFKPFTTAGALTHGYKTTDRLDCSSSLTVGSRVFKNY
                     ESAAYGPLTFAQALQLSCDTFFYRIGYAKWLASGADDSNVHIKDDLVENAKSFGFGSK
                     TGIDLPGEVSGRIADRMWKLSYWKANKDYYCKLGNQPGTDYLHVFAREFCTDGYRYRA
                     GDAVNFAIGQGDTQLTPLQLAVAYAAISNGGTLWEPRVGKAVVNAKGKVVRRIPPKAK
                     GHVPVPKRYLKYIDQALLGTAKVGTMAWKMVGFPLDTVKVRSKTGTAEVYGKQTTSWV
                     ASYDKRYVVVMQISQGGTGSGTSGESVRKIWETLYGVRGENVDTARAAEPGARPPTRL
                     PVFHHNGAITPPVLRAPGRDYDHRLLRTRTRPSQSRRTSW"
     gene            complement(9183..9695)
                     /gene="mreD"
                     /locus_tag="VH419_05650"
     CDS             complement(9183..9695)
                     /gene="mreD"
                     /locus_tag="VH419_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEY7043135.1"
                     /translation="MTVGLRLAAVAALIGLTVTMQVSIFTHLEIDGVVPDLALLLVIA
                     AALVRGPDYAALVGFAAGLVLDLAPPADHTAGRWALSLVLVGYLTGLVRGDGAGNALS
                     IVMTVAAGAFIATSVFALTGLVLQDPGVTVSSALEVVPVAVLYDVLLAPLVVPLVLVL
                     FRRLEPADRW"
     gene            complement(9692..10645)
                     /gene="mreC"
                     /locus_tag="VH419_05655"
     CDS             complement(9692..10645)
                     /gene="mreC"
                     /locus_tag="VH419_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012919932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEY7043136.1"
                     /translation="MPPQVGRSRTVLGLLVLASVTIITLDARHESSASPVDPLRAAVG
                     SVLGPAETAANDALRPLTSVSEHFRTVDSLRADNAELTAENKLLRSQLHTADANTRRS
                     TESAGLADLGDTSGFNIVAAQVVAMGSAQSFSRTVTIDAGTADGVVPDLTVVNADGLV
                     GRVIEATQRSATVLLIVDRKSTIGGRLGDSMELGFLTGDGDLAGDGTLELSLVDHTVS
                     PREGDSVVSWGSHHGAPYVAGVPIGKVVSVHSSPAELTETATVEPYVDFSSLDVVGVV
                     TQAPSARAQTLTNATSHATGHATNPASDKTTDKASDEERDR"
     gene            complement(10648..11673)
                     /locus_tag="VH419_05660"
     CDS             complement(10648..11673)
                     /locus_tag="VH419_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEY7043137.1"
                     /translation="MAGSIIGRDMAVDLGTANTLVYVRGRGVLLNEPSVVAMNAQSRE
                     ILAVGAEAKRMIGRTPDSITAIRPLKDGVIADFEATEQMLRFFIHRVHRRRYFAKPRL
                     VVCVPSGITPVEQRAVKEAGYQAGARRVYIIEEPMAAAIGAGLPVHEATGNMVVDIGG
                     GTTEVAVISLGGIVTSKSIRTAGDDLDQSIITWMKKEYSLMLGERTAEEIKLAVGSAF
                     PLPDEPEAEIRGRDMVSGLPRNVVISSAEVRQALEEPLHAIIDSVRTTLDKTPPELAG
                     DIMDRGVVLTGGGALLRGLDERLRHETGMPVHVADAPLDSVALGAGKCVEEFEALQQV
                     LVSEPRR"
     gene            complement(12070..12477)
                     /gene="ndk"
                     /locus_tag="VH419_05665"
     CDS             complement(12070..12477)
                     /gene="ndk"
                     /locus_tag="VH419_05665"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEY7043138.1"
                     /translation="MSERTLVLIKPDAVRRGLVGQVLARFESKGLSIVAMDLRTISGE
                     EADRHYAEHVERDFYPPLRAFVTSGPLVAAVLEGDEAVEVVRLLNGATDGRKAAPGTI
                     RGDWSLSNRENLVHGSDSLDSATREITLWFPHL"
     gene            complement(12488..12814)
                     /locus_tag="VH419_05670"
     CDS             complement(12488..12814)
                     /locus_tag="VH419_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4233 domain-containing protein"
                     /protein_id="HEY7043139.1"
                     /translation="MRSPRRMMCASVLGFECIVLGLVTPVLITVEDMAKPLALTIGLG
                     LAVAAVVVAGLLRFEWAYYAGFAVQAAALALGFVVPEMFVLGVVFGGLYVTAYVLGRR
                     LEAQPG"
     gene            complement(12814..14208)
                     /locus_tag="VH419_05675"
     CDS             complement(12814..14208)
                     /locus_tag="VH419_05675"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HEY7043140.1"
                     /translation="MPPSPSASSESSDAGDVLEQYREVERAMLARWPETKLDPSLDRI
                     AALCDLLGQPQRAFPVLHLTGTNGKTSTARMIDTLIRALGLRTGRFTSPHVESMTERI
                     SVDGEPVSRRRFTELYDEVEPYAAIIDRSGGHPLSFFELVTAMAFAAFADAPVDAGIL
                     EVGMGGSWDATNVADAAVAVITPIGVDHARYLGDTPADIAVEKAGILKQGTFGVLAQQ
                     PVEAAEVLLRHAAEVGATVAREGLEFGVLERKPGMGGQLVTLQGLGATYDEVFLPLYG
                     AHQAHNAVCALAAVEAFAGGKGLDSGLDAEVVRQAFGQVTSPGRLEVVRRSPTVVLDA
                     AHNPHGARASADAVGESFAFSPLIGVVGVMADKDVEGLLDAYEPVLSRIVCTQNSTSR
                     AMPAESLGEIARGIFGPDRVDVLPRLDDAIEAGIAMAEDADSEGFALGSGGVLVTGSV
                     ITAGEARTLLGGGR"
     gene            14288..16903
                     /gene="valS"
                     /locus_tag="VH419_05680"
     CDS             14288..16903
                     /gene="valS"
                     /locus_tag="VH419_05680"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEY7043141.1"
                     /translation="MTETLPTRAPDTSTAGSRAVVVPERPALEGLEDKWTAVWAEQDT
                     YTFDRSKPREAIYSIDTPPPTVSGSLHVGSVSSYTHTDLIARYQRMRGKEVFYPMGWD
                     DNGLPTERRVQNFYGVRCDPSVPYDPDFTPPEKPDAKRQVPISRRNFIDLCEELTAQD
                     EVAFEHLWRHVGLSVDWSHLYTTINDDSRTASQRAFLRNLSRGEAYQAEAPTMWDVTF
                     QTAVAQAELEAREYAGHYYRIAFHADAGPVYVETTRPELVGACVALIAHPDDERYQPV
                     FGSTVRSPVFGVELPVLPHHLAEADKGAGIAMCCTFGDLTDVQWWRELQLPVRTIIGR
                     DGRIQRETPDWLSTGQAQAAYEHLAGKTVFSARETMVDLLRESSDLDGEPKPTQRMTN
                     FYEKGDKPLEIVATRQWYIRNGGREESLRAALLARGDSIVWIPPFMRHRYDNWVSGLN
                     GDWLISRQRYFGVPFPVWYALDADGEPDHDNPLLASEDRLPVDPSSDVPDGYDASQRG
                     VPGGFVGDPDVMDTWATSSLTPQIAGGWERDPDLFARVFPMDLCTHAHEIIRTWLFSR
                     VVRAHFEHEQTPWSHAMISGFVLDPDRKKMSKSRGNVTTPADLLEKYGSDAVRWRAAM
                     SRPGLDSSFDETTMKIGRRLAMKVLNASKFVLGMGDFHGDTSNITEAVDLAMIRRLRQ
                     VIEEATAAFDAYDYTTALETTERFFWTFCDDYVELVKERAYGARGDDAANSARIALNA
                     ALSVQLRLLAPILPFVTEEVWSWSHDGSIHRTSWPDAELDLPSAPQDPDVLVVVAEAL
                     AGVRGAKSVAKVSMRTEVSRVTISGPADRLALVEPGLADLKAAGRIIGDVHLSPREGQ
                     GDAIDVEAELVITDT"
     gene            complement(17010..>18050)
                     /locus_tag="VH419_05685"
     CDS             complement(17010..>18050)
                     /locus_tag="VH419_05685"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY7043142.1"
                     /translation="IALGVWGAVGGFGAAAGVLLGGVLTDFLSWEWIFFVNVPVGLVA
                     LGLVPMLLGESRDAAARSFDALGAVLVTTGLTTLVLAITQGRPWGWDSARTIATFVAA
                     AALLVGFVVWENRVREPLMRFGILRTKTLLGANVAGLILGTVLFSTFLMLTLYMQQVL
                     GYSAMKTGFAYLAVAGTSIIWANVAAALVNRVGVRPLIATGMALLSVSMLYFTRIDVG
                     DSYVTALLPGFLVIAMGIAFCFVPISIAALAGVRQDEAGLASGLINTTQQIGGALGIA
                     VLSSIAISATDDKLGAGVAVPDALVSGFQAAFWVGAATAAVGFVAALVLVRDSELAPV
                     QATEIEPVLEPV"
BASE COUNT         3036 a         6251 c         5976 g         2787 t
ORIGIN      
        1 agacatcgag gaccgcaccg aggacttccc tcacgggtcg cgacgaccgg tgatcaggga
       61 tgcgggcgga tcaggagcga gctggccggg acgaagccgg ctcggcgata gaacggtatc
      121 gaacgctcgc tcgggctgag cacgacacga acgtagccat gctcgtcggc gtactccaac
      181 gcagcgtcga ggagtagctt ccccaccccg gcgtcacggt ccgcctgctc gacgtacacg
      241 ttcgcgatgt acccccattg acgtggatgc tgggggacgc cgacgggccg cggcttcggc
      301 atccgagtga acaccagcat gttgagcatc ccgatgatgc agtcgccgat ctcggccagc
      361 caggtgacac gttgatgggc ctcgcgttcg aaccacgcag cgaactccgc gtcgtagtcc
      421 tcgtcctcga tcggcgaccc tgcctgctcc tcgacccagg cccgtcggag ccgtgcaatc
      481 tcggcggcgt cacggatccc ggccacccgg atacggggac tcaccagcgt tcgaccaggt
      541 gctcgcggcc cgccggtggt tcgtacggct caggccagaa gtccgtgacc tggctgatca
      601 agccgtcgtc gaacccgaag aacgccatcg cgtcctgctc ccggtcgtcg ccgacccgcc
      661 atccgaacca ggccactccg tgctgctcgt cggcaatcag cactttcagg cgtaggtgcc
      721 agtcaccggg atactcctgg ttgaactgga ggtacgtgtc ccgaccccgg atgcgttccc
      781 gcgattgtgg cagctcgtag acgacgtcgg cgtgcatggt ggcggtccac cgatcccagt
      841 cgcgcgcctc gagtgacgag acgaacgtct cgacgagctg tcgcgggtgg gcggctccgg
      901 cgtactccgg tgtggtcacg ccgtcaccgt acaagcacgg catgtcggtc cggtgtgtgg
      961 gaaaccccgt gcggccgact cggatcactg tccccatgtc cgggcacact gagcgaattc
     1021 ccgtccgggc ccacctcgcc cggatggatc tgccgaacga acgacgaaag gtgaggatcg
     1081 atgactggat acaccgttgt gcatctggac gagatcgagg agttcgcgga cgcaggctgt
     1141 cactaccggc cgatcaggca ccacttgggc atcacggcgt tcggagtgat cgcctggtcg
     1201 gcccgtgcgg cgggcgaccg cgtcatcaac gagcacgacg aggacgaccc caccgctgac
     1261 caggagctct ttctcgtgct acgcggccac gcgttgttcg agctcgacgg tgaccgggtc
     1321 gacgcaccgg ccggaacgct gctgttcgcc ccgcccggca cgaagcgaat ggcctccgcc
     1381 acggaagccg gcaccaccgt cctcgcgttg gaaggcacac ccggaagggc gtacgaggcg
     1441 cgcggctggg agctctgggc tccgctcgcc ccgcttcacg cagcgggcga gtacgccgag
     1501 gtcgccgacc gtcttggcgc cctggtggcg gccaacccgc agtaccccat gctgttcttc
     1561 aacctcgcgt gctgcgaaag tctctgcggc cggacgaggg aggcactcga tcacctgaag
     1621 cacgccatcg agatgtcgga ggagtttcgc caatcggcga gggacgactc cgacctggat
     1681 ccgatccgcg acgaacccga cttcaagcag ctggtcagcg gctagcgtcg cggacgctcg
     1741 gacgccgagc gtctcccgtt cagaccggct ccttgacgct cagcggcaag aacgtcctgc
     1801 ccgtcggacc catctggatc tcggtcccca tctccgggca gatggatcac accacaaaac
     1861 agcgcgagcg gatgaaccgt tctggagatc caggctcact gtgctggata ccagcggatg
     1921 cacacggcat cgagtatcag cgacacggta gacgcgcccg tggccgcaca cgccgccgat
     1981 gcgaggcgct ctgctgcatc aaggacctca agccgctcac ctgggggcat ggtgcggacg
     2041 tgcgacacgg ttgacacata cccgcgccac tcgcggggag tcagtgacca gttccaccgg
     2101 accacttcct gctcggcgtc tcccgtcgtg aggccaaggc ggtccagcgg cggcagcgcc
     2161 cgcactcggg cagcgatgtc cgcactgagg ttgacggcct cccattgcca gtccggtgtc
     2221 gcagcggaca agtcgttcca gatacacccg agccacccac ctcgacgcag aactcgacca
     2281 acctcggctg ccgcctcagc gtttggaaac cagtgccagg cgtctgcgac caagaccgcg
     2341 tcgaccgaac catcttcgac cgggagattc gtgactgcgg cctcatgcac gggcaatgca
     2401 gagtggcgct cgctgacaag gcgcagcatc cggccatcaa tctcggtgac tcgcaacgag
     2461 acgtctttgt gctcgacgag tcgatcagtg agcttccctg tgcctgcgcc gacctcgact
     2521 acctcgcgcg cgtcaccgag aagccaattg acggcgctgt ccggatacga cggacggcaa
     2581 tcttggtact cgtccgccca tccgccgaat gaagtcgctc gcaccacccg cgtctcgtcc
     2641 acaaacgtgg atagtgccac ctttggtggt aactcgaagg accgaccacc ggacgccgct
     2701 gcgccgcgtc agatgcagat gcggcagatc cgcttcaaga cagacctgag tccatgtcgc
     2761 cgattgcttc tctgagttct ccatcgagat ggatcgaaca gagaaaccac ggcccgcacg
     2821 tggactcgcg gatactcgtt gcgctgtgtg cccaggtcag acgacagata agggcagtag
     2881 ccgctggccc gtcggaccca tctggatctc ggtccccatc tccgggcaca ccccgcagtc
     2941 gaagcagggg gtccatcggc agtcgtcaac ctcgacggcc ccggcggggt tcaacgcatc
     3001 ctgccagtcc tcccacagcc agtcccggtc caacccggaa tcgaggtggt cccaggggag
     3061 cacctcggag tactcccgct cgcgcaacgt gtaccagtcg aggtcgaccg gttcgtcgac
     3121 gagcgcccgc tcggatgctg cggcccaccg gtcgtacgag aagtgctcgc tccacccgtc
     3181 gaagcgtcct ccgtcacgcc aggcctcctc gatgatccgg ccgacccgac ggtcgccacg
     3241 tgacagcagt ccttcgacga tccctggctt gccgtcgtgg tagcggaatc cgatcgcgcg
     3301 gccgtactgc ttgtcggcac gcaccgtctc gcgcagcttc cgcagccgct cgtcagtcgt
     3361 ctcgtggtcg agctgcgacg cccactggaa cggcgtatgc ggcttcggca cgaacccgcc
     3421 gatcgacacg gtgcaccgga tgtcacgtcg gccggacgcc tcccgcccag tggcgatcac
     3481 cttcttggcc agctcggcga tctcgagcac gtccgcgtcg gtctcggtcg gcagcccgca
     3541 catgaagtac agcttcacct gtcgccagcc gtgggagtac gccgtcgcga ccgtgcgaat
     3601 caggtcctcc tcggagacca tcttgttgat gaccttccgc agccgttccg aacctccctc
     3661 cggtgcgaac gtcagccccg agcgccggcc gttccgggag agctcgttgg cgagcgtgac
     3721 gttgaaggcg tcgacgcggg tggagggaag ggacaacgag acgttcgtgc cgtcgtagcg
     3781 gtcggcgagg cctttcgcca cctcgccgat ctcactgtga tcggcgctgg acagcgacag
     3841 cagcccgacc tcctcgaaac cggacttctc gagaccgttc tggaccatgt cgccgatcgt
     3901 cgtgatcgag cgttcgcgca ccggacgcgt gatcatgccg gcctggcaga agcggcagcc
     3961 acgggtgcag ccacggaaga tctccacgga gtaccgctcg tgcacggtct ccgccaaagg
     4021 caccaacggc cggcgcgggt acggccactg gtcgagatcc atcagcgtgt gcttgtgcac
     4081 ccgccacgga acgccggcgc ggttgggcac gacccgtttg atgcggccgt cgggcaggta
     4141 gtcgacgtcg tagaagcgag ggacgtacac cccgccggac cgcgcgagcc gcatgagcag
     4201 ctcgtcgcga ccgcctggcc gcccctcgtc cttccactcg cggaccacgt cgctgatcgc
     4261 gagcacgatc tcctcgccat cacccagcac ggcggcgtcg aggaagtctg cgactggttc
     4321 ggggttgaac gccgagtgtc cgccggcgat cacgatcgga tgcgcgtcgt cacggtccgc
     4381 cgcatgcagc ggtatgccgg ccaggtccaa cgcggtgagc atgttcgtgt agccgagctc
     4441 ggtggcgaac gagatcccga ggacgtcgaa cgccccgacc ggtcggtgcg catccaccgt
     4501 gaactgcggg atgccgtgct cccgcatcac ctgttccatg tcggcgaacg cggcgtacgt
     4561 gcgttcggcg aggatccagt cgcgttcgtt gaggatctcg tacaggatct ggacgccctg
     4621 gttgggcagc ccgacctcgt aggcgtcggg gtacatcagg gcccatcgca ccgagacggc
     4681 gtcccagtcc ttcgcggtcg agttgagctc gccgccgacg tactggatcg gcttctgcac
     4741 ggtcggcagc aacggctcca gctgggcgaa cacggactcg acgggcatga ggcggggtac
     4801 tccaggcttg cttgtgggat cggtccaggt tacgcgcggc gcctgctgac gctcaaaaca
     4861 gggctccgca ggcccgcgct gtggtcaggt accggagtgg atcgcccagt tgaacggcgg
     4921 tgcgtccatc ccacgcgcga tccgcacgcg catccatgcc gacatcatca tctccggcgc
     4981 cgcggcggtc tcgatgcccc cgaggtcgac gatctgttgc ggccgccagc cgaagtcggc
     5041 gagcatcgcc gagacggtcc gcttggcagc gtcgtcgttg ccggacatga acacgacgtg
     5101 gtcgccctcg acgatcgacg ggtcggccat cacctggcag ttcatcgtgt tgagcgtctt
     5161 gacgacatgg gcgtcgggaa acgcgtcctg gagtgtctgg ccgagcgagc tgtccgcggt
     5221 ggcgcgtggc ttcgggtacc caccctcgac cggctcgagg tcgttcgtca cgtcgatcac
     5281 cggtttgccg gcgaggttct ccgcccctgc catgctcagc gcttccagcg cgtggctgcc
     5341 gttggtggcg ttgaggacga tctcaccgaa cgcggcggcg tcctcgaacg agccggtcgc
     5401 gagtcggtct gtctcagcga aggcggcgag gctgtccgag tcacgcgacc gtgcgccgac
     5461 catgacgtcg tagcccacgt cggcgagacg cttcgcgagc gtctggccga ccatcccggt
     5521 gccgaggacc ccgatcctgg tgcctgccat cgcctgaccc cttccgtcgt acgccgaatc
     5581 ggagtcagcg tacgaccgtg gcgcagcgat cggcgaaaac ggcgggacgt ggcatgcatg
     5641 ggctgtcggc gttgcagatg cttggaacac cccggcgagc gccgcgatgt ctacacggca
     5701 aactcactgt ccgcatcgtg ggtttgcgat gtttacagcc catctgggct gtaaacatcg
     5761 caaacccaca gcccgcacta gatgcggcgg gatcgggcga tgcggccacg gaggacgacg
     5821 ccgagcccga ggtggcgttc ggtgcgaagg tggatgttca acagcaggcc gatcgccatc
     5881 agcgacgcga acatcgacgt accgccgtag gacaccatcg ggagaggtac gccggtgacg
     5941 ggcatgatgc ccaggcacat accgacgttc tggaacgcct ggaacccgaa ccagcacacg
     6001 atgccggccc cggccaaccg accgaacatg tcgtcggcac gggtggcgat gcggactgcc
     6061 cgccacaaca gcacgaggta gagcgcgatc agtgcgccgg cgccgacgag cccgagctcc
     6121 tcccccgcca cagtgaacac gaagtccgtg tgctgctcgg gcacgaaccc ggactgcgtc
     6181 tgtgagcctt ggaagagccc ctggccgaac aacccaccgt tcccgatcgc gatccgcgcc
     6241 tgggtcgtgt tgtatccagc gccgcgcgga tccagtgtcg gatcgaggaa cgacccgaac
     6301 cggtcgagct gatacccctt cagcagtccg agctgcaccg ccgccacggc accggcgaca
     6361 gcgccgccgg cgagcaacac cagccagcgc ttcggtgctc ccgccacggc aacgagcccg
     6421 aacaccgtga cgcccagaac caggagcgtg ccgaggtccg gctgggccag gatgagcacg
     6481 gccggcacga ccacgagtgc gagcgcaccg acgacgtcgc ggtcccggag ctgtgacagc
     6541 cccttgcgat gctccgtctt ctcggcgagc agcagcgcaa gcccggcgac gacaccgagc
     6601 ttcgcgaact ccgcgggctg gatcgacatg ccgccgacgt ggatccacga ccgggacccg
     6661 ttgatcaccg cacccaccgg ggagagcacg agcacaagtc cgacgatggc ggcgaggtac
     6721 accaacggcg cccagatccg gacccaccga tggtccgtgg ccgcgacgaa gaacgccagc
     6781 accagcccga cggcgacgtt cgcgagatgt ctggtgaggt acgccgtgga gtccccaccg
     6841 gtgagggcgt cgttgttcgc ggtcgcggac cagacgagca gcgagcccat cgtgagcagc
     6901 gcggctgtgg ccaggaccac cacccagtcg agccgaagcc aggccgagcg ccgctcggtc
     6961 acgttcaccc gccgcgacgt ccgggtggtc gccacgtgcg ccgtcatcac catgacgtcc
     7021 gcctcgactg cgacggccgc gttcgtgtcc gcaacagtct gtggtcgtag tcgcggccgg
     7081 gggcgcgcag cacgggtggc gtgatggcgc cgttgtggtg aaacaccggc agccttgtcg
     7141 gcggccgggc gccgggctcc gccgcccggg cggtgtcgac gttctcgccc ctgacgccgt
     7201 acagcgtctc ccagatcttg cgcaccgact caccggacgt ccccgatccg gtcccgccct
     7261 gcgatatctg catgacgacg acgtaccgtt tgtcgtagct ggccacccac gacgtcgtct
     7321 gcttgccgta gacctcggcg gtgcccgttt tggatctcac cttgaccgtg tccagcggga
     7381 aacccaccat cttccatgcc atcgtcccga ccttcgccgt gccgagcagc gcctggtcga
     7441 tgtacttcag gtagcgcttg gggaccggca cgtgaccctt cgccttcggc gggatccgcc
     7501 tcaccacctt ccccttggcg ttgacgacgg ccttgccgac ccgcggctcc cacaacgtgc
     7561 cgccgttgga gatcgcggcg tacgcgacgg ccagctggag cggcgtgagc tgcgtgtcac
     7621 cctgtccgat cgcgaagttg accgcgtcgc cggcgcgata ccggtagccg tcggtgcaga
     7681 actcccgcgc gaacacgtgc aggtagtcag ttcccggctg attgccgagc ttgcagtagt
     7741 agtccttgtt ggccttccag tacgacagct tccacatccg gtccgcgatc cggccgctca
     7801 cctcgccggg gaggtcgatg ccggtcttgc tgccgaaccc gaacgacttg gcgttctcca
     7861 ccaggtcgtc cttgatgtgc acgttgctgt cgtcggcccc ggaagcgagc catttcgcgt
     7921 atccgatccg gtagaagaac gtgtcgcagc tgagctgcag cgcctgtgcg aaggtcagcg
     7981 gtccgtacgc cgcggactcg tagttcttga agacgcggct gccgaccgtg agactcgacg
     8041 agcagtccag cctgtccgtt gtcttgtagc catgcgtgag cgcgccggcc gtggtgaacg
     8101 gcttgaaggt cgaacccggc gcgaacaacc cctgcatcgg acggctcagc agcggggtgt
     8161 ccgccttcgg gctgtagaga cgttcgaggt caccctgggt gatgccgccg acccagacct
     8221 tcgggtcgta cgtcgggtag ctggccaacg cgacgacccg cccggtcttc gcgtccagca
     8281 cgacaacgga ccctgagtcg gcgacgtact tccggtgcgt gaccgggtcc gtggtctgcc
     8341 tggccaccat gatcgaacga acgagctccc tctcggccac ggcctgcacc ttggcgtcga
     8401 tgctcgtcac caacgtgtca cctggcctcg gcgcagtcat gccgtcgtac ccgagcacgc
     8461 ggcccatcga gtcgacggac acctcgcgca ccccgtcgtg gccgcggagg tagcggtcgt
     8521 aaccggcctc cagaccggct cgccccgtca gcgacagtgg gctgaggttg tggccgttct
     8581 gctccgcctt ggcgagctcc tgctcggtga tcggtgagat gtagccgagg acgtgagccg
     8641 cattgatgcc gtacggcgac gggtaggcgc gcaccttgcg tgcttcggcg gtgatccccg
     8701 gatagtcctc gttctgctcg aggatgctcg cggcgagccg ctgggtcacg tcctcggcga
     8761 ccggcacggg ttcgtatggc gagccgttcc agcagatggg cggcttggcc gcgccagcct
     8821 cgccacacgt cttcgtacgc cgctcgagct ccacccggga cagccccagg gtgtcggcga
     8881 gccgcgacag cacggcgttc cgcgtggagt caccgagctt cgccagcacg tcccggtcga
     8941 ctgtgacgac ccacgaggtg cggttggcga ccaacggccg gcccatcgcg tcgacgatca
     9001 ggccacgcgg cgcggggatg tcgatgtcgc ggatcgtgtt gtcctgggcg gccgcctgat
     9061 agtcggcacc ggcgacgacc tggatgtacc acaaccgcgc gaacaaggtg acgaacaagg
     9121 agagcaccat cgcctggacg acgaccaggc gcagccgaga gcgcgtcttc aacatgactt
     9181 gttcaccagc ggtcggcagg ttcgagccgg cggaacaaga cgagcacgag cggcaccacg
     9241 agcggggcca gcaggacgtc gtacagcacg gcgacgggga ccacctcgag cgccgatgac
     9301 acggtgacgc ctgggtcttg gaggacgaga cccgtgagtg cgaacaccga tgtggcgatg
     9361 aacgcgccgg cggcaacggt catgacgatg ctcagcgcgt tgccggcgcc gtcccctctg
     9421 acgagcccgg tgagataacc gacgaggacg agcgagagcg cccaccgccc ggccgtgtgg
     9481 tcggccggtg gtgccaggtc gaggaccagg ccggccgcga acccgaccag cgcggcgtag
     9541 tcgggaccgc ggaccagcgc ggccgcgatg accaggagca gcgcgaggtc gggcaccacc
     9601 ccgtcgatct ccaggtgcgt gaagatgctg acctgcatcg tgacggtcag cccgatgagc
     9661 gcggccaccg ccgcgagccg cagtccgacc gtcatcggtc gcgctcctcg tcgcttgcct
     9721 tgtccgttgt cttgtccgat gcggggttcg ttgcgtggcc cgtcgcgtgg ctcgttgcgt
     9781 tggtcaaggt ctgcgctctg gccgacggcg cctgtgtgac gacccccacg acgtcgaggg
     9841 acgagaagtc gacgtacggc tcgacggtgg cggtctcggt gagctcggcc ggtgacgagt
     9901 gcacgctcac caccttcccg atcgggacac cggcgacata cggtgcgccg tggtgcgagc
     9961 cccagctgac gacgctgtcg ccttcgcgcg gcgacaccgt gtggtcgacg aggctgagct
    10021 cgagcgtccc gtcgccggcg aggtctccgt cgccggtcag gaagccgagc tccatggagt
    10081 cgccgagtcg gccaccgatc gtggacttgc ggtcgacgat caacaagacg gtcgccgagc
    10141 gctgagtggc ctcgatgacc cgacccacca gcccgtcggc gttgacgaca gtgaggtcgg
    10201 gtacgacgcc atccgccgta cccgcgtcga tcgtcacggt gcgcgagaac gactgagccg
    10261 agcccattgc gaccacctgt gctgcgacga tgttgaaccc ggacgtgtcg ccgagatctg
    10321 cgaggccggc ggactcggtc gaccggcggg tgttggcgtc ggcggtgtgc aactgcgacc
    10381 gcagcagctt gttctcggcg gtcagctcgg cgttgtcggc tcgcaggctg tcgacggtcc
    10441 gaaagtgctc ggagaccgag gtgagcggcc ggagcgcgtc gttcgccgcc gtctcggccg
    10501 ggcccaggac tgagcccacg gcggcccgga gggggtcgac gggtgaggcc gacgactcgt
    10561 gccgagcgtc gagggtgatg atcgtcaccg aggcgaggac gagcagaccc aggacggtcc
    10621 gtgatcgtcc gacttggggc ggcacggtca ccgcctcggt tcggagacga gcacctgctg
    10681 caatgcctcg aactcctcga cgcacttgcc ggcgccgagg gcgaccgagt cgagcggcgc
    10741 gtcagccacg tggactggca tccccgtctc gtgccgcagc cgctcgtcga gaccgcgcag
    10801 cagagcaccg ccaccggtga gcacgacgcc acggtccatg atgtcgccgg ccagctcggg
    10861 tggcgtcttg tccaaggtcg tacggaccga gtcgatgatt gcgtgcagtg gctcctcgag
    10921 cgcctgacga acttccgccg acgagatcac cacgttgcgc gggagaccgc tcaccatgtc
    10981 ccggccgcgg atctcggcct ccggctcgtc gggcagcggg aaggctgagc ccaccgcgag
    11041 cttgatctcc tcggcggttc gctcccccag catgagggag tactccttct tcatccaggt
    11101 gatgatcgac tggtcgaggt cgtcgcctgc ggtgcggatc gacttgctcg tgacgatccc
    11161 gccgaggctg atcacggcga cctccgtggt gccgccgccg atgtccacca ccatgttccc
    11221 ggtggcctcg tggaccggaa gcccggcgcc gatcgccgcc gccatcggct cctcgatgat
    11281 gtagacgcgc cgggcgccgg cctggtaacc ggcctccttc acggcacgct gctccaccgg
    11341 agtgatgccc gacggcacgc agacgacgag ccgcggcttc gcgaagtagc gccgtcggtg
    11401 gacccggtgg atgaagaagc ggagcatctg ctcggtggct tcgaagtcgg cgatgacccc
    11461 gtccttgagg gggcggatcg cggtgatgct gtccggcgta cggccgatca tccgcttggc
    11521 ttcggcgccg acggccagga tctcgcggct ctgggcgttc atcgcgacga cggacggttc
    11581 gttcagcagc acgccgcggc cgcgcacgta gaccaaggtg ttggccgtgc cgagatcgac
    11641 cgccatgtca cggccgatga tgctgcctgc catttcgggt cgcctgcgtc cttcccgtcc
    11701 gcgccggtcg cgttcaggct cgttccgccc acggtcgagt tcaggctagg cgggtacgcc
    11761 gttcagatcg cggagacact tcgacacctg cgtaactttg tccccacccg ccccaccgtc
    11821 gagtgccagg ttcccgactg ttgagtgcgg ggtttgcggc cgttgagtgc tgagttgctg
    11881 agtgtcgaaa gcaggcttgt cagctgctga acgcgagttt cggactgagc ccgagtcttc
    11941 gacgttcgac ggccagcgac caagcattca acagcccggg aagcagcact caacagcccg
    12001 aaaggcagca ctcaacaacc cagaacccag cactcaacgg cccagaaggc agcactcaac
    12061 gaggaggggt cagaggtggg ggaaccagag ggtgatctcg cgggtggcgg agtcgagtga
    12121 gtcggagccg tggacgaggt tctcgcggtt cgacagcgac cagtcgccgc gaatggtgcc
    12181 gggcgccgcc ttccgtccgt cagtggcgcc gttgagcaac cggacgacct cgacggcctc
    12241 gtccccctcc aggacggccg cgaccaacgg gccactcgtc acgaacgcgc gcagcggcgg
    12301 gtagaagtca cgctcgacgt gctcggcgta gtggcggtcg gcctcttcac cggagatggt
    12361 ccgcaggtcc atggccacga tcgacagccc cttggactcg aaccgcgcca gcacctgacc
    12421 caccagacca cggcggacgg cgtcgggctt gatcaggaca agcgtgcgct cgctcatggc
    12481 gagcactcta accaggctgg gcctcgagcc tgcgccccag cacgtacgcc gtgacgtaca
    12541 accctccgaa gacgacaccg agcacgaaca tctcaggtac gacgaagccg agcgccaacg
    12601 ccgcagcctg aaccgcgaaa ccggcgtagt acgcccactc gaaccgcaac aacccggcga
    12661 ccaccacggc ggccaccgcc agaccgagac cgatcgtgag cgccaacggc ttggccatgt
    12721 cctcgaccgt gatcagcacc ggcgtgacca gcccgagcac gatgcactcg aacccgagca
    12781 cggacgcaca catcatccga cgcggcgaac gcatcagcga ccgccaccga gcagcgttcg
    12841 cgcctccccc gccgtgatga ccgagccggt gaccagcacg ccgccgctgc cgagcgcgaa
    12901 gccttcgctg tcggcgtcct ccgccatcgc gataccggct tcgatcgcat cgtcgagccg
    12961 gggcagtacg tcgacccggt ccggcccgaa gatgccccgc gcgatctcgc ccaacgactc
    13021 ggccggcatc gcccgggagg tggagttctg ggtgcacacg atgcgcgaca gcaccggttc
    13081 gtatgcatcg agcaggccct cgacgtcctt gtcggccatc acaccgacga cgccgatgag
    13141 cggactgaac gcgaacgact cccccaccgc gtcggcactc gcccgagcgc cgtgcgggtt
    13201 gtgcgccgcg tcgagcacca cggtcggtga gcggcggacg acctcgagcc gtccgggcga
    13261 ggtcacctgc ccgaaggcct gacgcaccac ctcggcgtcg agcccgctgt ccagtccctt
    13321 gccacccgcg aacgcctcca ccgccgccag cgcacagacg gcgttgtgcg cctggtgcgc
    13381 gccgtacagc ggcaggaaga cctcgtcgta cgtcgccccg aggccctgca aggtgacgag
    13441 ctgtccgccc atgccgggtt ttcgctcgag cacgccgaac tcgagccctt ctcgggccac
    13501 cgtggcgccc acctccgcgg catggcggag cagcacttcg gccgcctcca ccggctgctg
    13561 cgccaggact ccgaacgtgc cctgcttcag gatccctgcc ttctccacgg cgatgtcggc
    13621 cggcgtgtca ccgaggtagc gcgcatggtc gaccccgatc ggcgtgatga ccgcgacggc
    13681 tgcgtccgcg acgttggtgg cgtcccagct gccgcccatc ccgacctcga ggatcccggc
    13741 gtcgacgggc gcgtcggcga acgccgcgaa cgccatcgcg gtgacgagct cgaagaacga
    13801 cagcgggtgc ccgccggacc ggtcgatgat cgcggcgtac ggctccacct cgtcgtagag
    13861 ctcggtgaac cggcggcgac tcactggttc accgtcgaca ctgatgcgtt cggtcatcga
    13921 ctcgacatgc gggctggtga accggccggt gcgcagcccg agcgcccgga tcagcgtgtc
    13981 gatcatccgc gccgtcgagg tcttaccgtt ggtgccggtg agatggagga cggggaaggc
    14041 gcgttgtggc tggccgagca ggtcgcagag cgcggcgata cggtccaggg acgggtccag
    14101 cttcgtctcc ggccaccgag ccagcattgc ccgctccacc tcgcggtact gctcgagtac
    14161 gtcgccggcg tcagatgact ccgaagaggc cgaaggggaa gggggcatcg tgcccgcagt
    14221 ctatcgacgc agttggcggg tgaactcgtt cgcggtaccg agaaggccgc acgtaagatt
    14281 tcgcgctatg acagagaccc ttcccacccg cgctcctgac acgtcgaccg ccggttcccg
    14341 tgcggtcgtc gtaccggagc gtccggcgct ggaagggctc gaggacaagt ggacggcggt
    14401 gtgggccgag caggacacgt acaccttcga ccgcagcaag ccgcgcgagg cgatctactc
    14461 catcgacacg ccaccgccga cggtgtccgg gtcgctgcac gtcggaagcg tcagctccta
    14521 cacgcacacc gacctgatcg cgcgctacca gcgcatgcgc ggcaaggagg tcttctatcc
    14581 gatgggctgg gacgacaacg gccttcccac ggagcggcgc gtgcagaact tctacggcgt
    14641 gcggtgcgac ccgtccgtcc cgtacgaccc ggacttcacg cccccggaga agcccgacgc
    14701 gaagcggcag gttccgatca gtcggcgcaa cttcatcgat ctgtgcgagg agctcaccgc
    14761 ccaggacgag gtggccttcg agcacctctg gcggcacgtc ggactctcgg tcgactggtc
    14821 acacctgtac acgacgatca acgacgacag ccgcaccgcg tcccaacgcg ccttcctccg
    14881 caacctgtca cgcggcgagg cataccaggc cgaggcgccg acgatgtggg acgtcacgtt
    14941 ccagaccgcg gtcgcgcagg ccgagctcga ggcgcgcgag tacgccgggc actactaccg
    15001 catcgcattc cacgccgacg ccggaccggt gtacgtcgag acgacgcggc ctgagctcgt
    15061 cggtgcctgc gtcgcgctga tcgcccatcc ggacgacgag cgctatcagc cggtgttcgg
    15121 ctcgaccgtg cgctcacccg ttttcggcgt ggagctgccg gtgcttccac atcatctggc
    15181 cgaggccgac aagggcgccg gcatcgcgat gtgctgcacg ttcggtgacc tgaccgacgt
    15241 ccagtggtgg cgcgagctgc agctgccggt gcgcaccatc atcggtcggg acggccgcat
    15301 ccagcgggag acgccggact ggctgtccac gggacaggcc caggcggcgt acgagcacct
    15361 cgccggcaag acggtgttca gcgcgcgcga gacgatggtc gacctgctgc gcgaatccag
    15421 cgatctcgac ggtgagccca agccaaccca gcggatgacg aacttctacg agaagggcga
    15481 caagccgctg gagatcgtgg cgacccgaca gtggtacatc cgcaacggcg gccgcgagga
    15541 gtcactgcgc gcagcgttgc tggcccgcgg agactccatc gtctggatcc cgccattcat
    15601 gcgacatcgg tacgacaact gggtgagcgg gctgaacggc gactggctga tctcccgcca
    15661 gcgctacttc ggagtgccgt tcccggtctg gtacgcactc gatgccgacg gcgagcccga
    15721 ccacgacaac ccgctgctgg catcggagga ccggctgccg gtcgacccgt cctctgacgt
    15781 gccggacggg tacgacgcgt cgcagcgcgg cgtgcccggc ggcttcgtcg gcgacccgga
    15841 cgtgatggac acctgggcca cgtcgtcgct cacgccgcag atcgccggcg gctgggagcg
    15901 agacccggac ctgttcgcgc gcgtcttccc gatggacctg tgcacgcacg cccacgagat
    15961 catccgcacc tggctgttct cgcgcgtcgt acgcgcgcat ttcgagcacg agcagacgcc
    16021 gtggtcgcac gccatgatct ccggcttcgt cctcgaccct gaccggaaga agatgtcgaa
    16081 gtcgcggggc aacgtcacga caccggcaga cctgctcgag aagtacggct cggacgccgt
    16141 gcgctggcgg gcggccatgt cgcggcctgg gctcgactcg tcgttcgacg agaccacgat
    16201 gaagatcggt cgccggctgg ccatgaaggt gctcaacgcg tcgaagttcg tgctcggcat
    16261 gggcgacttc catggcgaca cgagcaacat caccgaagcc gtcgacctgg cgatgatccg
    16321 ccgactccgt caggtgatcg aggaggcgac cgcagcgttc gacgcatacg actacacgac
    16381 ggcgctggag acgaccgagc ggttcttctg gacgttctgc gacgactacg tcgagctggt
    16441 gaaggagcgc gcgtacggcg ctcgaggtga cgacgccgcg aactccgccc gaatcgcgtt
    16501 gaacgccgcg ctgtcggtcc agctccggct gctggcaccg atcctgccgt tcgtcaccga
    16561 ggaggtgtgg tcgtggtccc acgacgggtc catccaccgc accagctggc cggatgcgga
    16621 gctcgacctg ccgagtgcac cgcaggaccc ggacgtcctc gtcgtcgtgg ccgaggcgct
    16681 cgccggcgtt cgcggtgcca agtcggtggc gaaggtgtcg atgcgtaccg aggtcagcag
    16741 ggtcaccatc agcggacccg ccgaccggct cgcactggtc gaaccggggc tcgccgacct
    16801 gaaggccgcc ggtcgcatca tcggcgacgt ccacctgtcg ccacgcgagg gccaaggtga
    16861 cgccatcgac gtcgaagccg agctggtgat caccgacacc tgagccggtg ccggcctgac
    16921 cgcgaattgc gggaagttcc gcgattccga gctgcgaaat cgcggaactt cccgcattcc
    16981 tgaagcggac acccccgccg aggttcggct cagacgggtt cgagcacggg ctcgatctcg
    17041 gtggcctgca ccggcgcgag ctcggagtcg cggaccagca ccagcgcggc gacgaagccg
    17101 accgctgcgg tcgcggcacc gacccagaac gccgcctgga aacctgacac cagcgcgtcc
    17161 ggcacagcca cccccgcacc gagcttgtcg tcggtggccg agatggcgat ggacgacagc
    17221 accgcgatgc cgagtgcgcc accgatctgc tgggtggtgt tgatgagacc tgacgccagt
    17281 cctgcctcgt cctggcgtac gccggccagc gcggcgatcg agatcggcac gaagcagaac
    17341 gcgatgccca tggcgatcac gaggaacccg ggcagcagcg ctgtcacgta cgagtcgccg
    17401 acgtcgatgc gcgtgaagta gagcatcgac accgacagca gcgccatccc ggtcgcgatc
    17461 aacggacgca cgccgacccg gttcaccagc gcggcggcca cgttggccca gatgatggag
    17521 gtgccggcga cggccaggta cgcgaacccg gtcttcatcg ccgagtaccc gagcacctgc
    17581 tgcatgtaca gcgtgagcat caggaacgtc gagaacaaca cggtgccgag gatcagcccc
    17641 gcaacgttcg ccccgagcag ggtcttcgtg cggaggatgc cgaaccgcat cagtggttcg
    17701 cggacccggt tctcccacac gacgaagccg accagcaggg cggccgcggc cacgaaggtc
    17761 gcgatcgtac gcgcggagtc ccagccccag ggacggcctt gggtgattgc caggacaagt
    17821 gtcgtgagcc cagtcgtgac gaggacggcg ccgagcgcgt cgaagctgcg cgcggcggcg
    17881 tcgcggctct cgcccaacag catcggcacc aggccgagcg cgacgagacc gacgggcacg
    17941 ttgacgaaga agatccactc ccaggagagg aagtccgtca acacaccgcc gagcaggacg
    18001 ccagccgcgg cgccgaagcc accgacggcg ccccagacac cgagcgcgat
//
LOCUS       DATBFZ010000187         4599 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_2746526, whole genome shotgun sequence.
ACCESSION   DATBFZ010000187 DATBFZ010000000
VERSION     DATBFZ010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 4599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4599
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2746526"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            173..1063
                     /locus_tag="VH419_05690"
     CDS             173..1063
                     /locus_tag="VH419_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009074940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEY7043143.1"
                     /translation="MTQTSMREQFAATTVELLDADPSVALVLADISERFFRDAMRHHP
                     ERVINVGIREQLAVNVGAGIALTGMRPIVHTFASFLIERSFEQIKLGFAHQGVGGVLV
                     SAGASYDNSESGRTHQSPADVALIDTLPDWEIHVPGHPAELDALLRQAVAGPGPVYIR
                     TSDRSNRSPQPTGGLQVLDRGGQGTVVVVGPLLDNVLAAVPDLDVTVLYASTVRPFDA
                     DTLVATLDRADVVLVEPYAVGTSAAAVSAALADVPHRLKAIGVPKVELRRYGTSAEHE
                     TAYSLDPAGLRRTITDFLGH"
     gene            1158..1904
                     /locus_tag="VH419_05695"
     CDS             1158..1904
                     /locus_tag="VH419_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008361152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEY7043144.1"
                     /translation="MEAASGGRPARLAMWSGPRNISTALMRSWENRRDTVVVDEPLYP
                     HFLAATGIDHPGRDEVIAVGETDWQVAVEGLLAPLPNDVAVFYQKHMAHHLLPGMEHD
                     WMRSLTNVLLIRDPREVVASYVRSREHVTADDIGLPQQTMLYDEWVAWGETPHVIDAA
                     DFLRDPEAYLRRLCDLVEVEFTNRMLSWPAGPRDTDGVWGPYWYDAVWKSTGFAPYRA
                     REIHLEGASAEVAEQCLPHYEQLHAHRWTP"
     gene            1966..2382
                     /locus_tag="VH419_05700"
     CDS             1966..2382
                     /locus_tag="VH419_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEY7043145.1"
                     /translation="MNTTHGSPAEPSLGELVTRLSEQTSRLVRDELRLAQAEMKESAK
                     QAGLGAGLFSAAGVLGLFGFGTAIATAIIALALALPWWLSALIMTVVLFVAAAVAGVL
                     GRRHVQQVSPKPERAIENVKRDVQEVKESAQHDHTH"
     gene            2366..2728
                     /locus_tag="VH419_05705"
     CDS             2366..2728
                     /locus_tag="VH419_05705"
                     /inference="COORDINATES: protein motif:HMM:NF023697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3618 domain-containing protein"
                     /protein_id="HEY7043146.1"
                     /translation="MTTPTDPTTAAPTPEPDKNATADEIAADIERTREHLGETVDALA
                     RKADVKAQAAARVDEAKVRAREQAQHVAAVGQRVATKAKESATDDEGHVKPAFPVGVA
                     SAAVLFVFAWLIWRRRRS"
     gene            2698..3774
                     /locus_tag="VH419_05710"
     CDS             2698..3774
                     /locus_tag="VH419_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013160018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEY7043147.1"
                     /translation="MADLETTAIMSRHRPTAELGERPDPDDPRKPHSPTDLRKPSWKY
                     VARTTAREFTKDECTDIAAALTYYAILAMFPAVIAMMSLVGLFASGPKSVDTLLGILS
                     DVGGSSIADTLRPTLEQLSKTPGAGVALVLGLAGALWSASGYVGAFGRAMNRIYEIRE
                     GRPFWKLRPVMLAITFVLLLLAAAAVVGLVITGPVATSVGDAIGLGSTVVTVWDIVKW
                     PVMLLVVMLIVAILYWATPNVQQPRFRWLSIGAAVAIVVWIVLTVAFGFYVANFSSYD
                     RTYGSLAGVIVFLLWLWITNLALLFGAELDAELERARQLQAGIAAEESIQLPPRDTRN
                     IEKATAKAEADERRGHELREDSDS"
     gene            3874..>4599
                     /locus_tag="VH419_05715"
     CDS             3874..>4599
                     /locus_tag="VH419_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017932887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEY7043148.1"
                     /translation="MSRHSASNLDQRDEPPADDSEPGGPTDLTMASWRYVARRTGREF
                     SKDECTDVAAALTYYAILAVFPAVIALMSLVGLFANGPNSIDKLLDIVSDVGGSSVAD
                     TLQPTLEKLSRAPGAGLALVLGLAGALWSASGYVGAFGRAMNRIYDVGEGRPFWKLRP
                     LMLAVTFVLVLLAAAAMLGLVVSGPVAKSIGDAVGIGSSVVTVWDIVKWPVILLVVMM
                     IVAILYWATPNVQQPKFRVISIGA"
BASE COUNT          745 a         1520 c         1590 g          744 t
ORIGIN      
        1 agcgccgttt cgacatcgag ggatggtcca cgcagcgcgt cgacggacgc gaccacgcag
       61 cgctgcgggt cgcgctgggt cacaccgacg accaccggcc gacactcgtc gtggcagaga
      121 tcgagggata cggatgacca cctcactgac gacaaggacg accgtgacgg acgtgacaca
      181 gacgtcgatg cgcgaacagt tcgccgccac aaccgttgag ctgctcgacg ccgacccgtc
      241 ggtggcgctg gtgctggcgg acatctccga gcggttcttc cgcgatgcga tgcgtcacca
      301 ccccgagcgg gtgatcaacg tcgggatccg cgagcagctg gcggtgaacg tcggagccgg
      361 gatcgcgctg accggcatgc ggccgatcgt ccacacgttc gcgtcgttct tgatcgagcg
      421 gtccttcgag cagatcaaac tcggcttcgc gcaccagggc gtcggcggag tcctggtcag
      481 cgccggcgcg tcgtacgaca actcagagag tggccgaacc caccagagcc cagcggacgt
      541 ggccctcatc gacacgctcc cggactggga gatccacgtc ccgggtcacc cggccgagct
      601 ggacgcgctg ctccggcagg cggtggccgg tccggggccg gtctacatcc gcaccagcga
      661 tcgctcgaac cggtcacccc agcccaccgg cggcctccag gtgctggacc gtggcgggca
      721 agggaccgtg gtcgtcgtgg gaccgctgct cgacaacgtg ctcgccgcag tcccggacct
      781 cgacgtcacc gtcctgtacg cctcgacggt ccggccgttc gacgcggaca cgttggtcgc
      841 gacactcgac cgggccgacg tggtcctggt cgagccgtac gccgtcggga cgtctgctgc
      901 ggcggtgtcg gcggcgctgg cggacgtgcc gcaccgactg aaggcgatcg gcgtaccgaa
      961 ggtcgagctg cggcgatacg gcacctctgc cgaacacgag acggcgtaca gcctcgaccc
     1021 ggccggactg cgccggacga tcaccgactt cctgggacac tgatcagcgc cgcagcccgg
     1081 tgatctcgac aggctcgatc cgcgggcatc cgacgggctc gggcagcgaa gctgtgggcg
     1141 cgtgagatcg tggcgccatg gaggcggcga gcggcgggcg accggcccgg ctcgcgatgt
     1201 ggtccggccc gcgcaacatc tcgaccgcac tgatgcggtc ctgggagaac cgacgcgaca
     1261 ctgtggtggt cgacgagccg ttgtatccgc acttcctggc cgcgaccggc atcgaccatc
     1321 ccggtcgcga cgaggtgatc gcggtcggtg agaccgactg gcaggtggcc gtcgagggcc
     1381 tgctcgcacc tctcccgaac gacgtcgcgg tcttctacca gaagcacatg gcgcaccacc
     1441 tgcttcccgg gatggagcat gactggatgc gttcgctcac caacgtgctg ctgatccgcg
     1501 atccacgcga ggtcgttgcg tcgtatgtca ggtcccggga gcacgtgacc gccgacgaca
     1561 tcgggctgcc acagcagacg atgctgtacg acgagtgggt ggcttggggc gagacgccac
     1621 acgtcatcga cgccgccgac ttcctacgcg acccggaagc gtatctgcgg cgtctgtgcg
     1681 acctggtgga agtggagttc acgaacagga tgctgagctg gccggccggt ccgcgcgaca
     1741 ccgacggcgt gtgggggccg tactggtacg acgcggtgtg gaagtcgacc ggcttcgcgc
     1801 cgtaccgggc tcgcgagatc cacctggagg gggcgtcggc ggaggtggcg gagcaatgcc
     1861 tgccgcacta cgagcagctc cacgcccacc gatggacgcc ctagaaaggc aagaacctcg
     1921 tccacggtgg gtgtgaggta ccgcccggat gggcatatcg aggcgatgaa caccacacat
     1981 ggttcgccgg cagagccttc gctcggcgaa ctcgtcacca ggctctcgga gcagaccagc
     2041 cgcctcgttc gtgacgagct gcgactggct caggcggaga tgaaggagtc ggccaagcag
     2101 gccggcctcg gcgcgggtct cttcagcgct gccggtgtgc tgggcctgtt cgggttcggc
     2161 acggcgatcg ccaccgcgat catcgcgctg gctctcgcgc tgccgtggtg gctctccgcg
     2221 ctcatcatga ccgttgtctt gttcgtggcc gccgccgtcg ccggtgtcct gggccgacgg
     2281 cacgttcaac aggtttcacc gaagcccgaa cgggcgatcg agaacgtcaa gcgggacgtt
     2341 caggaagtca aggagagtgc gcagcatgac cacacccact gatccgacga ccgccgcgcc
     2401 cactccggag cctgacaaga atgcgaccgc ggacgagatc gccgccgaca tcgagaggac
     2461 ccgagagcac ctgggcgaaa ccgtcgacgc gctcgcacgc aaggccgacg tcaaggcgca
     2521 ggccgccgcc cgcgtcgacg aggccaaggt tcgagcgcga gagcaggcac agcacgtcgc
     2581 agcggtcggc caacgggtcg ccaccaaggc gaaggagtcc gccactgacg acgagggtca
     2641 cgtcaagccg gccttccccg tcggggtggc atcggccgcg gtgctgttcg tgtttgcgtg
     2701 gctgatctgg agacgacggc gatcatgagc aggcatcggc cgaccgcgga gctgggcgag
     2761 cggccggatc cggacgatcc gcggaaacca cattcgccca ccgacctgcg caaaccctcc
     2821 tggaagtacg tcgcccggac gaccgcgcgg gagttcacca aggacgagtg caccgacatc
     2881 gcggcagcgt tgacgtacta cgccatcctc gccatgtttc cggcggtcat cgcgatgatg
     2941 tcgctcgtcg gactgttcgc gagtgggccg aagtcggtcg acacgctcct cgggatcctg
     3001 tccgatgtgg gcgggtcgtc gatcgcggac accttgcggc ccacgctgga gcagctgagc
     3061 aagacgccag gcgcaggcgt tgccctcgtc ctcggtctcg ccggcgccct ttggtcggcg
     3121 tcggggtacg tcggcgcgtt cggacgggcg atgaaccgca tctacgagat acgcgaaggc
     3181 agaccgttct ggaagcttcg tccggtgatg ctcgctatca cgttcgtgtt gttgctgctc
     3241 gccgcggctg cggtcgtcgg tctcgtcatc accggccccg tcgcgacctc ggtcggtgac
     3301 gcgatcgggt tggggtccac cgtggtgacc gtgtgggaca tcgtgaagtg gccggtcatg
     3361 ttgctcgtcg tgatgctgat cgtcgccatc ctctactggg caaccccgaa tgttcagcag
     3421 cccagattcc gttggctcag catcggggcc gcggtcgcaa tcgtggtgtg gatcgtcctg
     3481 acggtcgcgt tcggcttcta cgtcgcgaac ttctccagct acgacaggac ctacggctct
     3541 ctcgccgggg tcatcgtctt cctgctgtgg ctgtggatca cgaacctcgc cctattgttc
     3601 ggtgccgagc tcgacgcgga gctcgagcgc gcccgacagc tccaggcggg gatcgcggcc
     3661 gaggagtcca tccagctacc accgcgcgac acccgcaaca tcgagaaggc gaccgcgaag
     3721 gcggaggccg acgagcgccg cggccacgag cttcgggagg actcagacag ctgatacctc
     3781 tgacgcggcg tgagagccgg accgcttggt gccctgtctg aggcgacctt tcttgggcat
     3841 gatgagcaac agggcaacgc aaagggggat gtcatgagcc gccactccgc gtccaacctc
     3901 gaccagcgcg acgaaccgcc ggccgacgac agcgaacctg gaggaccgac cgacctgacg
     3961 atggcctcgt ggcgttacgt cgcacgcagg accggccggg agttctccaa ggacgagtgc
     4021 acggatgtgg ctgccgcgct cacctactac gcgatccttg ccgtcttccc cgccgtgatc
     4081 gcgctcatgt cactggtcgg gctcttcgcg aacgggccga actcgatcga caagctgctc
     4141 gacatcgtgt ccgacgtcgg tggatcctcg gtcgcggaca cgctccagcc gacgttggag
     4201 aagctcagcc gggctcctgg ggcggggctc gcgctcgtgc tcggtctcgc cggagcgttg
     4261 tggtcggcgt cggggtacgt cggggcgttc gggcgggcga tgaatcggat ctacgacgtc
     4321 ggcgaaggcc ggccgttctg gaagctccgc ccgctcatgt tggccgtgac gttcgtgctc
     4381 gtgctgctcg ccgcagccgc catgctcggc ctggtcgtga gcgggccggt cgcgaagtcg
     4441 atcggcgacg ccgtcggcat cgggtcatcg gtggtgacgg tctgggacat cgtgaagtgg
     4501 ccggtgatcc tgctggtcgt gatgatgatc gtcgccatcc tctactgggc gacgccgaac
     4561 gtgcagcaac cgaagttcag ggtgatcagc atcggcgcc
//
LOCUS       DATBFZ010000188         2633 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_782126, whole genome shotgun sequence.
ACCESSION   DATBFZ010000188 DATBFZ010000000
VERSION     DATBFZ010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_782126"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..845)
                     /locus_tag="VH419_05720"
     CDS             complement(<1..845)
                     /locus_tag="VH419_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043149.1"
                     /translation="MGAARRRLVTAVVTAAIVVASAAIPAPATEGRQVPHRWRPAVSQ
                     TRVDSKYPQAGDPRVDALHYRLGLRWAPATRTLTGHEALLWRATRTMRTFALDLSPDL
                     RLRSVRLDGHRVVHTRRHHKLHLHARVRRGSVHRLVLVYAGVPHGVRGGPIPGAGAQV
                     TPSGGLWTCQPPYGAFTWYAVNDQPADKAYYDFRLAVPRPLVGVANGRLIDRHRHGRL
                     VRTRWHLDAPASSYLTTLGFGRYMHTSLGRVHGTPVSLWTPRGDAATRRKFRYAAPAL
                     RWLEHR"
     gene            complement(1083..1505)
                     /locus_tag="VH419_05725"
     CDS             complement(1083..1505)
                     /locus_tag="VH419_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012919561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEY7043150.1"
                     /translation="MLMRTDPFRDFDRLTEALWGTRTRPAVMPLTAYRDDSTFVVHLD
                     LPGVSADSIDLTVEQNVLTVHAERKAPVGDTAERVIDERSYGVFSRQLFLGDTLDVDQ
                     LNADYDAGVLTIKIPIAEKAKPRKVEITGGSSRKEINA"
     gene            1688..2131
                     /locus_tag="VH419_05730"
     CDS             1688..2131
                     /locus_tag="VH419_05730"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEY7043151.1"
                     /translation="MHRLDPALQTALELLDVGADANPGQVAEAYRRKARTAHPDVNDA
                     PDAVASFQDLAAAYRIALEAARRHETAERIRIGTSGLRPPDETLVGADDVPTHHDPPL
                     VLGVVEDQPAPRGRSATSTPLVAGPVVIHPPASNTEQDPSSGGAR"
     gene            2131..2580
                     /locus_tag="VH419_05735"
     CDS             2131..2580
                     /locus_tag="VH419_05735"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEY7043152.1"
                     /translation="MPDLSDDRALYSISVTSELSGVNPQMLRSYEQRGLLAPYRTEGG
                     TRRYSGNDLDRIEEITTLLAAGLNLAGVEQVLRLRAETRQLQQEIDQLRNRPAARPDG
                     QEVTRLKAENRALRGEVTRLQDVDPDTASERRPQPSAHPRPKSPKTP"
BASE COUNT          420 a          894 c          927 g          392 t
ORIGIN      
        1 cggtgttcga gccagcgcag cgccggcgcg gcgtaccgga acttgcgcct ggtggccgca
       61 tcgccccggg gggtccacag cgacaccggc gtgccgtgaa cccggcccag cgacgtgtgc
      121 atgtagcgcc cgaagccgag cgtggtgagg tacgacgacg ccggagcgtc gaggtgccag
      181 cgggtgcgca ccagccgtcc gtggcggtgc cggtcgatga ggcggccgtt tgcgacgccg
      241 accaacggcc tgggcacagc cagtcggaag tcgtagtagg ccttgtcggc gggctggtcg
      301 ttgacggcgt accaggtgaa cgcgccgtac ggcggctggc aggtccacaa cccgccactc
      361 ggcgtcactt gcgctcctgc gccgggaatc gggccgccgc ggacaccgtg cgggacgccg
      421 gcgtagacga gcacgagccg atggacgctt ccgcgccgga cccgggcgtg caggtgaagc
      481 ttgtggtggc gccgcgtgtg aacgacccga tgaccgtcga ggcgcaccga ccgcagccgc
      541 aggtcggggg acaggtcgag cgcgaacgtg cgcatggtgc gggttgcccg ccacagcagg
      601 gcctcgtggc cggtgagggt gcgggtggcc ggcgcccatc ggagcccaag acggtagtgc
      661 agcgcatcta cgcgggggtc gccggcctgc gggtacttgc tgtcgacgcg ggtctgcgag
      721 acggcaggac gccaccggtg cggcacctgc cgaccctcgg tcgcgggagc ggggatcgcg
      781 gcgctggcga cgacgattgc cgcggtcacc accgcggtca cgagtcgacg ccgggctgcg
      841 cccacctcgc caccatgaca ccgctgtcaa ggctggcggt cggtccgtct ggcgtgtgtg
      901 gcgagccttg tcctgacgtg gaatcccgat ggccgacgag cgtggtgacg gcgggtgggt
      961 gtgaccggcc tgaccggcca cacccaccgt gaggtagagc ggcctccgtt cgtccagcgg
     1021 tcagcgcgtg cgcgtggtgt acccggagag cggccttacg tctgtgatca ggccacgcat
     1081 tgtcaggcgt tgatctcctt gcggctggat ccgcccgtga tctccacctt gcgcggcttc
     1141 gctttctccg cgatcgggat cttgatggtc agcacgccgg cgtcgtagtc ggcgttgagc
     1201 tggtcgacgt cgagggtgtc acccaggaac agctggcggc tgaagacgcc gtacgagcgc
     1261 tcgtcgatca cgcgttcggc ggtgtcgccg accggagcct tgcgctcggc gtggacggtc
     1321 agcacgttct gctccacggt caggtcgatc gagtcggccg agactccggg aaggtcgaga
     1381 tgcacgacga acgtgctgtc gtcacggtag gcggtcaagg gcatcacggc cggacgtgtc
     1441 cgggtgcccc acaaggcttc ggtgaggcgg tcgaagtccc ggaacgggtc ggtacgcatc
     1501 agcatcgtgg ttcctcctca cgtggtccgg ctggcgacga gcctgatggt ggcatcgcca
     1561 gagaatctct gcttgatact tcagattttg ctcacctgat gcataatgtc aagcggtaca
     1621 gcgacatgtt tttcagccgc gctcgcgaga cgagccgggc gtcgagtcca ggcaggaggt
     1681 ccgtgagatg caccgtctgg atcctgcgct gcaaacggcc ctggaactgc tcgacgtcgg
     1741 tgcggatgcc aaccccggcc aggtcgccga ggcgtaccga cgcaaggcca ggaccgccca
     1801 tccggacgtc aatgacgccc cggacgcggt cgccagtttc caggacctgg cagccgccta
     1861 ccgcatcgcg ctcgaggcgg cgcgtcggca cgagaccgcc gagcgcatcc gcatcggcac
     1921 gtccggcctc cgaccgccgg acgagaccct cgtcggcgcc gacgacgtcc ccacgcacca
     1981 cgaccctccg ctggtcctcg gcgtcgtcga ggaccagccc gcaccacgtg gtcggtcggc
     2041 aacgagtacg ccgctggtcg ccggaccggt cgtgatacac ccgcccgcga gcaacaccga
     2101 gcaggatccg tcgtccggag gagcgcggtg atgcctgacc tgagcgacga tcgcgcgctg
     2161 tactccatct cggtgacctc tgagctcagt ggcgtcaacc cgcagatgct gcgcagctac
     2221 gagcaacgcg gcctgctcgc gccgtaccga acggaaggtg gcacccggcg atacagcggc
     2281 aatgacctgg accggatcga ggagatcacc accctgctcg ccgccggctt gaacctcgcg
     2341 ggtgtcgagc aggtgctcag gctgcgggcg gagacccgac agctccagca agagatcgac
     2401 cagctgcgca accgccccgc cgccagacca gacggccagg aagtgacacg cctcaaagcg
     2461 gagaaccgtg ccctccgcgg agaagtcact cgactgcagg acgttgaccc ggacacggcc
     2521 agcgagcgcc gacctcagcc gtccgcgcat cctcggccca agagtccgaa gactccgtga
     2581 cccgacagca tcggcgccgc ttcgtcgttt cacacctcac ccggaggtcc tcg
//
LOCUS       DATBFZ010000189         4691 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_1111302, whole genome shotgun sequence.
ACCESSION   DATBFZ010000189 DATBFZ010000000
VERSION     DATBFZ010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 4691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4691
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1111302"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="VH419_05740"
     CDS             <1..366
                     /locus_tag="VH419_05740"
                     /inference="COORDINATES: protein motif:HMM:NF016840.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HEY7043153.1"
                     /translation="KQWADFIVNGPSRGPDVDWANIDWSNPPAEVLEYQNGFRMVGDE
                     TLAGLLTAYDEVAAATDELVRTVDLDARQTLPEAPWFEPGSSWSARRTFMHIVAETAQ
                     HAGHADIIRETIDGQKSMG"
     gene            444..517
                     /locus_tag="VH419_05745"
     tRNA            444..517
                     /locus_tag="VH419_05745"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:478..480,aa:Pro,seq:ggg)
     gene            complement(607..1086)
                     /locus_tag="VH419_05750"
     CDS             complement(607..1086)
                     /locus_tag="VH419_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017935076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YetF domain-containing protein"
                     /protein_id="HEY7043154.1"
                     /translation="MEIVIRALVVFVFLWLVTRAVGRTTLGELSAFELLLYVTMGDLV
                     QQAVTQQDYSVTGGLLAVSTFAILTVFLSWLQARFGRLRPVITGRPVLVFADGRPIEG
                     AMRQQRLTLADLLAAAREQGIRRTSEIEYALLEADGRISFFTSSEDGEGAPERPPTG"
     gene            complement(1130..1648)
                     /locus_tag="VH419_05755"
     CDS             complement(1130..1648)
                     /locus_tag="VH419_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HEY7043155.1"
                     /translation="MDVPSSTIEGVETSAHGRLAIWTRRGFLTLLTVFVLAGVAGLLG
                     VRTSTADSHADGWGLSLSYAATARAGLDVPWQVTVTHEGGFGKDLQLAVTGDYFDIYE
                     TQGFNPEPSDVTRDGDLMYLTFTAPPGDTFVLRYDAYIQPSSQAGAGGTVSVIDQGQR
                     VVSVDFDTVLLP"
     gene            complement(1699..2244)
                     /locus_tag="VH419_05760"
     CDS             complement(1699..2244)
                     /locus_tag="VH419_05760"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HEY7043156.1"
                     /translation="MPDPGELWTIGHWTCPESAFLEFLGGPGITLLADVRSQPGSRRS
                     AQFDRDEMPRWLASAGIDYLHLPDLGGRRGRQPVDESVNAGWRQRSFHNYADYTLGET
                     YQRGVDRLAELAATERVAIMCGEPMPWRCHRLLIANTLAARDWTIWHLMGDAPPRRHV
                     LGQWGAQPSIDGDGVVTYPAE"
     gene            complement(2249..2998)
                     /locus_tag="VH419_05765"
     CDS             complement(2249..2998)
                     /locus_tag="VH419_05765"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY7043157.1"
                     /translation="MSNTRDLKATVALVTGAGSGLGRATSRELAAHGAHVLAADIDVD
                     GAKATVEMVDRDGAGSAEAIELDVTDRVAVDEVMRSASATYGERFDCLVSNAGTDRGD
                     DLVNVEDEAWHRVFGVNVHGPMYLARGFVRHLQRLDSTRTGDIVCIVSISALTVGAGA
                     GAYNSSKAAMLKLAEVMQTEAREQHWPARVSILNPAAMNTPMMEQWQLAEDRMMDPQI
                     VAGLVRTAITLPPEVVLQNAVVTLRNEYYPR"
     gene            3198..3626
                     /locus_tag="VH419_05770"
     CDS             3198..3626
                     /locus_tag="VH419_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009077214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEY7043158.1"
                     /translation="MTVAREIMTEGADCARTTDTLVDAAKKMKDLDVGALPICGEDDR
                     LHGMITDRDIVVDCIAAGGDPSSAKVEEFSQDETVTIGADDSVEEALRTMIDHGVRRL
                     PVIDGHQLIGMVSQADVARHLPEDQVATLVEAISSSPANN"
     gene            complement(3660..>4691)
                     /locus_tag="VH419_05775"
     CDS             complement(3660..>4691)
                     /locus_tag="VH419_05775"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF and ANTAR domain-containing protein"
                     /protein_id="HEY7043159.1"
                     /translation="MMSMLPVPALITDGYGRIRALNAAAAQLLDIGVSRAIGKPLAGY
                     VSAEDRSSLRRIISRAAQTHDYLREIVRFTPRHSETVTVECSLGLLPGSQTELRWTLL
                     SAHRAEEARDPELGGHLAEALARLAGLAVGFPDRQSLLHQAAEVIDGVLGKGISLTVS
                     VGPPDNPEAVSSTSQLAQSVDGAQLAAGEGPCSTAFKTEFTVVSNDIHHDPRWPRLAP
                     LVDAEVRAVLAVPLEVGDELVGALNIYAEAELSAEVVEAAELIAATLAAVLLEFTTRE
                     ELQGLADDMEAALTSRATIDQAKGIVMAERGVGPDEAFQHLVDLSSTTHTKLREVAQS
                     IVRAVQRSD"
BASE COUNT          744 a         1601 c         1645 g          701 t
ORIGIN      
        1 aagcagtggg ccgacttcat cgtgaacggg ccgagccgcg gccccgacgt cgactgggcg
       61 aacatcgact ggagcaaccc tcccgccgag gtgctggagt accagaacgg cttccggatg
      121 gtcggcgacg agacgctcgc cggactgctc acggcgtacg acgaggtcgc ggcggccacc
      181 gacgagctgg tccgcaccgt cgacctcgac gcccgccaga cgctgcccga ggcgccgtgg
      241 ttcgagcctg gctcgtcctg gtcggcacga cgtaccttca tgcacatcgt cgccgagact
      301 gcccagcacg ccggccacgc cgacatcatc cgggagacca tcgacggaca gaagtcgatg
      361 ggctgacctg agcggaccga cccgaattcc ggctcggctc gccgtctgcg gtagggttca
      421 cgcggccttc gagagaaggt cttcgggctg tggcgcagtt tggtagcgca cttgactggg
      481 ggtcaagggg tcgcaggttc aaatcctgtc agcccgaccg atgcgatgtc ccgcgacacc
      541 gttgacgggt gtctcgggac atcgttgcat cggggtcctc gcagtggctc ttgtgagggg
      601 gccgtctcaa cccgtcggag gacgctccgg cgctccctcc ccgtcctcgc tgctcgtgaa
      661 gaaggagatg cgtccgtcgg cctcgagcag ggcgtactcg atctccgacg tacgccggat
      721 gccctgctct cgtgcggctg ccaggaggtc tgccaaggtc aggcgctgct ggcgcatcgc
      781 cccctcgatc ggtcgtccgt cggcgaagac caggaccggc cggccggtga tgacgggccg
      841 cagccggccg aagcgggcct gcagccagga caagaagacg gtgaggatgg cgaacgtgct
      901 gaccgcgagg agaccaccgg tgaccgagta gtcctgctgg gtcacggcct gctggacgag
      961 atcgcccatg gtgacgtaga gcaacagctc gaacgccgag agctcgccca gcgtcgtgcg
     1021 cccgaccgcg cgggtcacca gccacaggaa cacgaagacg accagggcac ggatcacgat
     1081 ctccacggga cgctcccttc tcccggcatg cctggccgag tgctgaggct catggcagga
     1141 ggaccgtgtc gaagtcgacc gacaccacgc gctgtccctg gtcgatgacg ctgacggttc
     1201 ctccggcgcc ggcctggctg gagggttgga tgtaggcgtc gtacctcagc acgaacgtgt
     1261 cgcccggcgg agccgtgaac gtgaggtaca tcaggtcgcc gtcacgggtg acgtccgagg
     1321 gttcggggtt gaagccctgg gtctcgtaga tgtcgaagta gtcgccggtg accgcgagct
     1381 gcaggtcctt gccgaaacct ccctcgtggg tcaccgtcac ctgccacggc acgtcgaggc
     1441 cggcacgggc cgtggcggcg tagctgagtg agagtcccca cccgtcagca tgcgagtccg
     1501 cggtcgacgt gcggacgccg aggagtcctg cgacgccggc cagcacgaac acggtcagca
     1561 gggtcaagaa cccccgacgg gtccagatcg ccagccggcc gtgcgccgag gtctcgacac
     1621 cctcgatcgt cgacgaaggc acgtccacga ctcgtgtcta acaccgggcc tcggttcgag
     1681 atcgccaaca cgccgggatc actccgccgg gtaggtgacg actccgtccc cgtcgatcga
     1741 gggctgcgcg ccccactgac ccagcacgtg gcgtcgagga ggtgcgtcgc ccatcaggtg
     1801 ccagatggtc cagtcccgtg ccgccagcgt gttcgcgatg aggagccggt ggcaccgcca
     1861 cggcatcggc tcgccgcaca tgatcgcgac ccgctcggtc gcggcgagct cggcgagccg
     1921 gtcgacacca cgctggtagg tctcgccgag cgtgtagtcg gcgtagttgt ggaagctgcg
     1981 ctgccgccag ccggcgttga cggactcgtc gaccggctgg cggccacgcc gaccgccgag
     2041 gtcgggcaga tggagatagt cgatcccggc ggatgcgagc cagcgcggca tctcgtcgcg
     2101 gtcgaactgc gcgcttcggc gggatccggg ctgggagcgt acgtcggcca gcagcgtgat
     2161 gcccggtccg ccgaggaact ccaagaacgc cgactccggg cacgtccagt ggccgatcgt
     2221 ccacagctca cctgggtccg gcaacggttc accgcgggta gtactcgttg cgcagcgtga
     2281 cgaccgcgtt ctgcaggacc acctccggcg gcagggtgat cgccgtacgg accaggccgg
     2341 cgacgatctg cgggtccatc atccggtcct cggcgagctg ccactgctcc atcatcggtg
     2401 tgttcatcgc cgcggggttg aggatgctga cccgtgccgg ccagtgctgc tcacgagcct
     2461 cggtctgcat cacctccgcc agcttgagca tcgctgcctt cgaggagttg taggcgccgg
     2521 cgccggcgcc gacggtcagc gcgctgatcg acacgatgca gacgatgtcg cccgtccgcg
     2581 tgctgtcgag gcgctgcagg tgccggacga accctcgagc caggtacatc ggcccgtgca
     2641 cgttgacccc gaagacccga tgccacgcct cgtcctcgac gttcacgagg tcgtcgccgc
     2701 gatcggtgcc ggcgttgctg accaggcagt cgaaccgctc gccgtacgtg gccgacgcgc
     2761 tgcgcatcac ctcgtcgacc gcgacgcgat cagtgacgtc cagctcgatc gcctcggcgc
     2821 ttcccgcccc gtcgcgatcg accatctcca ccgtcgcctt cgccccgtcg acatcgatgt
     2881 cggccgccag cacgtgcgca ccatgggcgg ccagctcgcg actcgtcgcg cgacccaagc
     2941 cgctcccggc gccggtcacg agagcaacgg tcgccttcag gtctcgggtg ttcgacatcg
     3001 tgcgcccctt ctccatcgat gagccaggcg atgcaggtac cccgcagcga gatcgtcagt
     3061 cctgacaccg gggcctgtcc cgcaacccca tggccgagga ccacggggtc agacggcatg
     3121 cgaccccgga ggccgggtac tgaccccgtc catcgaccac gcgccgcacg gcggcgagag
     3181 gacggagagg tgaggacatg acggtggcac gcgagatcat gaccgaaggc gcggactgtg
     3241 cccggacgac cgacacgctg gtcgacgcgg cgaagaagat gaaggacctc gacgtcggag
     3301 cgctgcccat ctgcggcgag gacgaccggc tgcacggcat gatcaccgac cgcgacatcg
     3361 tggtcgactg catcgccgcg ggcggcgacc cgagctcggc gaaggtggag gagttctctc
     3421 aggacgagac ggtcacgatc ggcgccgacg actcggtcga ggaggcgttg cgcacgatga
     3481 tcgatcacgg cgtacgacgg ctgcccgtga tcgacggcca tcagctgatc gggatggtca
     3541 gccaggccga cgtggccagg cacctgcccg aggaccaggt ggcgacgctg gtcgaggcga
     3601 tctcgtcgtc ccccgcgaac aactgacgcc gagcacgccg tagcccgaac gctggggagt
     3661 cagtccgagc gttgcacggc ccgcacgatg ctctgcgcga cctcgcgcag cttcgtgtgg
     3721 gtggtgctgc tgaggtcgac gaggtgctgg aaggcctcgt cggggccgac gccgcgctcg
     3781 gccatgacga tgcccttcgc ctggtcgatg gtcgcgcgcg aggtgagtgc ggcctccatg
     3841 tcgtcggcca ggccctggag ctcttcgcgg gtggtgaact ccaggagcac ggcggccagg
     3901 gtcgcggcga tcagctcggc ggcctcgacc acctcggccg agagctccgc ctcggcgtag
     3961 atgttgagcg ctccgacgag ttcgtcgccg acctccaggg gtacggcgag caccgctcgc
     4021 acctcggcgt cgaccagggg cgcgagccgt ggccaacgcg gatcgtggtg gatgtcgttc
     4081 gagaccaccg tgaactcggt cttgaacgcg gtggagcacg gcccctcccc ggcggcgagc
     4141 tgcgcgccgt cgactgactg ggccagctga gaggtcgacg agaccgcctc ggggttgtcc
     4201 ggggggccga cggagaccgt cagcgagatg cccttcccga ggacgccatc gatgacctcg
     4261 gcggcctggt ggagcaggct ctggcggtca gggaagccga cagccagacc ggcgaggcga
     4321 gcgagggcct cggccaggtg accgccgagc tccggatccc gcgcctcctc cgcccgatgg
     4381 gcgctcagca gggtccagcg cagctcggtc tgcgatccgg gcagcagacc gagggagcac
     4441 tcgactgtca cggtttcgct gtgtcgggga gtgaacctca cgatctcgcg gaggtagtcg
     4501 tgcgtctgcg cggcgcggct gatgatccgt cggagcgacg accggtcctc ggccgagacg
     4561 taccccgcga ggggcttgcc gatcgcccgc gagacgccga tgtcgagcag ctgtgccgcc
     4621 gccgcgttga gcgcccggat ccggccgtac ccgtcggtga tcagggccgg caccgggagc
     4681 atcgacatca t
//
LOCUS       DATBFZ010000190         3816 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_455307, whole genome shotgun sequence.
ACCESSION   DATBFZ010000190 DATBFZ010000000
VERSION     DATBFZ010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 3816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3816
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_455307"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="VH419_05780"
     CDS             complement(<1..255)
                     /locus_tag="VH419_05780"
                     /inference="COORDINATES: protein motif:HMM:NF019220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HEY7043160.1"
                     /translation="MNWFDTGDYIEPGRLWLLLVVPVLLVLYVLLQRRKSRFAVRFTN
                     VALIDTVVRRQVNWMQHIAVGLALLTLAFGVVLFAKPTKQV"
     gene            complement(252..1241)
                     /locus_tag="VH419_05785"
     CDS             complement(252..1241)
                     /locus_tag="VH419_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015070209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEY7043161.1"
                     /translation="MPKPQPASTLALSDLAPEAALRRLELQIVRKLEGFLHGEHLGLL
                     PGPGTDLAEARYYVPGEDDVRRMDWSVTARTTTPHVRDVIADRELETWALVDMSASMH
                     FGTAALEKRDLAVAAVATMGLLTFRVGDRFGAVVLRDGRSRRWPARTGRFALFELLRS
                     LMREAPADGTAPAQTLTEAIAALARRPRRRGLRVVVSDFLDRDDSFGDVEAPPSWERP
                     LRQLATRHHVIGVEIIDPRELDIPDVGTILMRDAETGQVRELHTSSRRVRQTYAAAAR
                     LQRERTARAMRRAGVGHLVLRTDRPWLTEFARFALAHRRMAADLRSRRTGVLR"
     gene            complement(1244..2311)
                     /locus_tag="VH419_05790"
     CDS             complement(1244..2311)
                     /locus_tag="VH419_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY7043162.1"
                     /translation="MTTSPAQTTGTGTGSSPVQHLTADELSRLSDLAQRSMQEVKRVI
                     VGQEHMVEQLMVALIARGHCLLEGVPGTAKTLAVRTFADVVGGSFTRIQFTPDLVPSD
                     IVGTRIYRPSKESFDVELGPVFVNFVIADEINRAPAKVQSAMLELMAEHQVSIGGQTF
                     ELPDPFIVIATQNPIESEGVYPLPEAQRDRFLFKVDIQHPRGQEEFEILRRMSVDPPK
                     ASGVLDPTTILELRHAADHVFLHNLVSEYIVRLVLATRVPAEFQLHDLDGVIEIGVSS
                     RATLGLAAAARAVALIHGRTYVLPQDVAVVARDVMAHRLVLSFDAVADEVDPRFVVDR
                     VVSAIPPPQAVWNNSPRPEFR"
     gene            2453..3178
                     /locus_tag="VH419_05795"
     CDS             2453..3178
                     /locus_tag="VH419_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043163.1"
                     /translation="MTEGKVAPTPALLPSALLKTDPPAVGDVVLSGRLGSHDAGTVYA
                     GSVDGADVTVVMLTEGAETDSYARARFRQATSELAAEHPGVILATEDEEDLAPWVAMA
                     STSWLEGDALAGRLMRAVTLEDRPSIGTVTGPDFRPHWWHRVGVGRWRVWPLPWPARL
                     LSAGRWTFVASFALVCAIAALALWLAVQVFQNQVPPPPGPGPGPGPLPPPSPTSPSPT
                     PTPSPIPGQQQGPTGAPPAPPIV"
     gene            3316..>3816
                     /locus_tag="VH419_05800"
     CDS             3316..>3816
                     /locus_tag="VH419_05800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043164.1"
                     /translation="MRRAFPFESPIAPDALIDRRDELRQLHLAATDGAHVRLAGPRRY
                     GKTSLLLAHAEHLRGAGWHTVHVDFYGVTSLLEVCSRIATAYGRLKDHRIRSHLESLG
                     SRLGVSLTTSGVGVTLGPQLPKQPSYDAALTAAAELLDLPLRLYDKDRAQTLVVFDEF
                     QDLLSAG"
BASE COUNT          599 a         1327 c         1312 g          578 t
ORIGIN      
        1 gacctgcttc gtgggcttgg cgaacagtac gacgccgaac gccaacgtga gcagcgcaag
       61 accgaccgcg atgtgttgca tccagttgac ctgacgccgt acgacggtgt cgatgagcgc
      121 gacattggtg aaccgtacgg cgaaccgact cttgcgacgc tgcagcagca cgtacagcac
      181 cagcaagacc gggacgacca gcaggagcca caagcggcca ggctcgatgt agtcaccggt
      241 gtcgaaccaa ttcatcgcag cacgcccgtc cgccgactac gaaggtcggc cgccatccgg
      301 cggtgggcga gcgcgaaccg ggcgaactcc gtcagccacg ggcggtcggt ccggagaacg
      361 aggtggccga cgccggctcg gcgcatggcc cgggcagtac gttcgcgctg tagccgagcc
      421 gcggcggcgt aggtctggcg cactcgccgg ctggacgtgt gcagttcacg cacctgaccc
      481 gtctcggcgt cgcgcatcag gatcgtgccg acgtctggga tgtcgagctc gcggggatcg
      541 atgatctcga ccccgatcac gtgatgccgg gtcgcgagct gacgcaacgg tcgttcccag
      601 ctcggcggcg cttccacgtc gccgaaggag tcgtccctgt cgaggaagtc ggagacgacg
      661 acccgcagcc cacgccgcct gggccgccgg gccagcgccg cgatcgcctc cgtaagcgtc
      721 tgggccggag cagtcccgtc cgccggcgcc tcccgcatga gtgaacgcag cagctcgaac
      781 agtgcgaacc ggccggtccg cgcgggccac cgacgcgagc ggccgtcgcg cagcaccacc
      841 gcaccgaacc ggtcccccac ccggaacgtc agcagtccca tggtcgccac cgccgccacc
      901 gcgaggtcgc gcttctccag ggccgcggtc ccgaagtgca tcgaggcgga catgtcgacc
      961 agcgcccagg tctcgagctc ccggtcggcg atcacgtccc gaacgtgtgg ggttgtcgtg
     1021 cgagccgtca ccgaccagtc catccgtcgt acgtcgtcct cgcctggcac gtagtagcgg
     1081 gcctcggcga ggtccgtgcc aggcccgggg agcaggccga ggtgctcgcc gtgcaggaag
     1141 ccctcgagct tgcggacgat ctgcagctcc agccgacgca gcgccgcctc cggcgcgagg
     1201 tcggaaaggg ccaacgtcga tgcaggctgc ggcttgggca cggtcagcgg aactccggtc
     1261 tcggcgagtt gttccagacc gcctgcggcg gcgggatcgc gctgacgaca cggtcgacga
     1321 cgaacctggg atcgacctcg tccgcgaccg cgtcgaagct cagcaccaac cggtgcgcca
     1381 tcacgtcacg cgccaccaca gccacgtcct gtgggagcac gtaggtccgc ccgtggatca
     1441 gcgccacggc gcgagcggcc gccgccagcc cgagcgtggc gcgcgacgac acaccgatct
     1501 cgatgacacc gtcgaggtcg tgcagctgga actcggcggg cacacgggtc gccagcacca
     1561 gccggacgat gtactccgac acgaggttgt gcaggaacac gtggtcggcg gcgtgacgca
     1621 gctcgaggat cgtggtcggg tcgagaacgc cggacgcctt cggtggatcg acgctcatcc
     1681 ggcgcaagat ctcgaactcc tcctggccgc gagggtgctg gatgtccacc ttgaagagga
     1741 accggtcacg ctgcgcctcc gggagcgggt acacgccttc ggactcgatg gggttctgcg
     1801 tcgcgatcac gatgaacggg tcgggaagct cgaacgtctg gccgccgatg gagacctggt
     1861 gttcggccat cagctccaac atcgccgact gcaccttcgc cggtgcacgg ttgatctcgt
     1921 cggcgatcac gaagttgacg aagaccggac cgagctcgac gtcgaacgac tccttgctgg
     1981 gtcggtagat gcgcgtcccg acgatgtcgg acgggaccag gtccggggtg aactggatgc
     2041 gagtgaagct gccgccgacg acgtcggcga acgtgcggac ggcgagcgtc ttggcggttc
     2101 ccggtacgcc ctcgagcaga cagtgaccgc gggcgatcag cgccaccatg agctgctcga
     2161 ccatgtgctc ctgcccgacg atgacacgtt tgacctcttg catggaccgt tgcgccaggt
     2221 cggacagccg ggagagctcg tctgcggtga ggtgctgaac gggcgacgaa ccggtgccag
     2281 ttccggtggt ctgcgccggg ctggtcgtca tgggtcgttc ccctcacggg cggttctcgt
     2341 cgtacgctct gggtacgctc tgcgtgcagg taagtcttac agacccgacc aaggctcccg
     2401 ccgccgtggg caccagcagg acacggtcag ccggacggac aggcggtgcg agatgaccga
     2461 gggaaaggtg gcaccgactc cagcgctgct gccctcggcc ctgctgaaga cagatccacc
     2521 ggcggtgggt gacgtcgtgc tctctgggcg actcggaagc cacgacgcgg ggacggtgta
     2581 cgccgggagc gtcgacggcg ccgacgtgac cgtggtgatg ctgaccgaag gtgcggagac
     2641 cgactcgtac gcccgcgcca ggttccggca ggcgacttcg gagctcgcgg ccgaacaccc
     2701 cggggtcatc ctcgcgaccg aggacgagga ggacctcgcc ccgtgggtgg cgatggcgtc
     2761 gacctcgtgg ttggaggggg acgcgctcgc cggccgcttg atgcgcgctg tcaccctcga
     2821 ggaccggccg tccatcggca ccgtcaccgg cccggacttc cggccgcact ggtggcatcg
     2881 cgtcggcgtc gggcgctggc gggtgtggcc gctgccctgg ccggcccggc tgctgtctgc
     2941 cggtcggtgg acgttcgtcg cgtcgttcgc cctcgtctgc gccatcgccg cgctggcgct
     3001 ctggctcgcc gttcaggtgt tccagaacca agtgccgccg ccgcccggcc ccggccccgg
     3061 tcccggcccg ctcccaccgc cgtcgccgac cagcccgtcg ccgacaccga caccgtcgcc
     3121 gatcccgggc caacagcagg gcccgaccgg cgccccgcct gcgcccccca tcgtctgaac
     3181 cgcgaattgc gggaaaacgg ctcctcagac tgggtattga acgtgacctt tcccgctatg
     3241 cagagtggct gttgtatggg tgatgacata catttctgtc aaggaattgc catacatctg
     3301 cggtaggctg aggagatgcg ccgcgcgttc ccgttcgaga gcccgattgc acccgacgcc
     3361 ctcatcgacc gccgagacga gcttcgacaa ctccacctgg cggccaccga cggcgcccac
     3421 gtgcgtctgg cgggtccccg ccgctacggc aagacctcgc tgctgctggc gcacgccgag
     3481 cacctgcgcg gcgcgggctg gcacaccgtg cacgtcgact tctacggtgt cacctcgctg
     3541 ctcgaggtgt gctcgcgcat cgcgacggcg tacggacggt tgaaggacca tcggatccgt
     3601 agccacctcg agtcgctcgg ctcccggctc ggggtcagcc tgacgacgtc cggtgtcggg
     3661 gtgacgctcg ggccgcagct gccgaaacag ccgtcgtacg acgccgcgct gacggcggcc
     3721 gccgagctgc tcgacctccc gctgcgtctc tacgacaagg atcgtgccca gaccctggtg
     3781 gtcttcgacg agttccagga cctgctcagc gcgggt
//
LOCUS       DATBFZ010000191         1577 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_4988679, whole genome shotgun sequence.
ACCESSION   DATBFZ010000191 DATBFZ010000000
VERSION     DATBFZ010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4988679"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(29..820)
                     /locus_tag="VH419_05805"
     CDS             complement(29..820)
                     /locus_tag="VH419_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017936719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEY7043165.1"
                     /translation="MRLGTFNVNSIRSRIGRVEALLDRHQIDVITLQETKARDDQWPL
                     MGLQALGYEVATLGYDQWNGVAIASRVGLTDVRHGFEGQPQWGEPLTSEARALAATCG
                     GVQVWSLYVPNGRAVGDPHLGYKLAWLEALRKAAMGWLADDPDAQVALTGDWNIAPTD
                     EDVWDMSVFAKSTHVTPPERAAFDAIVQTGYADLVRPYTPGPGTFTYWDYYRNRFERN
                     HGMRIDFVLGTPALQQRVTGATIDRDERAGKGASDHAPVFVDLAD"
     gene            complement(830..1414)
                     /locus_tag="VH419_05810"
     CDS             complement(830..1414)
                     /locus_tag="VH419_05810"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEY7043166.1"
                     /translation="MSNDLTWPRRTKQLELHPPNLTVIDEVLAVRNDPEMNRWFMVTS
                     AEPEPFRKAWLESVDDPDAYHLVAMSGGAVVGIASLEISDGMGQGMGGTDTESLPWRH
                     SEGSIDYMIKPAYAGQGYATEIVRDLLSLAFDDLGLRRVTGGCFADNIASWRVMEKAG
                     MRREQHGVQDSWHEELGWIDGYMYGILAEEWKAS"
     gene            complement(1506..>1577)
                     /locus_tag="VH419_05815"
     CDS             complement(1506..>1577)
                     /locus_tag="VH419_05815"
                     /inference="COORDINATES: protein motif:HMM:NF016840.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HEY7043167.1"
                     /translation="LREFAQHCGHADILREQILATDA"
BASE COUNT          253 a          565 c          490 g          269 t
ORIGIN      
        1 gagcgacagc agcgtctgac aactcgggtc agtcggcgag atccacgaag accggagcgt
       61 ggtcgctggc acccttcccg gcccgctcgt cccggtcgat cgtggcgccg gtgacgcgct
      121 gctgcaacgc cggcgtcccg aggacgaagt cgatccgcat gccgtgattg cgctcgaacc
      181 ggttccggta gtagtcccag taggtgaatg tccccggacc cggcgtgtag ggacgcacca
      241 ggtcggcgta ccccgtctgc acgatcgcgt cgaacgccgc tcgctccggc ggcgtcacgt
      301 gggtgctctt ggcgaacacg ctcatgtccc acacgtcctc gtcggtcggt gcgatgttcc
      361 agtcgccggt cagcgccacc tgagcatcgg ggtcgtccgc cagccagccc atcgcggctt
      421 tccgcagcgc ctcgagccag gccagcttgt agccaagatg tgggtcgccg actgcgcgac
      481 cgttcgggac gtacagcgac cagacctgaa cgccaccgca ggtcgccgcg agcgcccgag
      541 cctccgaggt gagtggctca ccccactgcg gctgtccctc gaaaccgtgg cgaacgtcgg
      601 tcagtccgac gcgggacgcg atcgcaacac cgttccactg gtcgtatccc agggtcgcca
      661 cctcgtagcc gagcgcctgg agccccatca gcggccactg gtcgtcacgc gccttcgtct
      721 cctgcagcgt gatcacgtcg atctggtgcc gatcgagcag cgcctcgacc cgaccgatgc
      781 gagagcggat cgagttcacg ttgaaggtcc ccaggcgcac cgcctcagcc taggatgcct
      841 tccactcctc ggcgaggatc ccgtacatgt acccgtcgat ccagccgagc tcctcgtgcc
      901 acgagtcctg cacgccatgc tgctcacgtc gcatgccggc cttctccatc acccgccacg
      961 acgcgatgtt gtccgcgaag caaccgccgg tcacccggcg cagcccgagg tcgtcgaacg
     1021 ccaacgacag caggtcgcgc acgatctcgg tggcgtagcc ctgaccggcg tacgccggct
     1081 tgatcatgta gtcgatcgac ccctcgctgt gacgccatgg cagcgactcg gtgtcggtcc
     1141 cgcccatgcc ttgccccatc ccgtcggaga tctcgagtga agcgatgccg accaccgcgc
     1201 cgccggacat cgccacgagg tgatacgcat cggggtcgtc gaccgactcg agccaggcct
     1261 tgcggaatgg ctccggttcg gccgacgtga ccatgaacca tcgattcatc tccgggtcgt
     1321 tgcggacggc gagcacctcg tcgatgaccg tcaagttcgg agggtgcagc tccagctgct
     1381 tggtgcgtcg tggccaggtc aggtcgttcg acatcggaac atcttcgacc cctcagtccc
     1441 tcgaacgcca ctgagttttc acgacgccga caccgaacct ccgtcacggc gtgagcgcga
     1501 ctccgtcagg cgtcggtcgc caggatctgc tcccgcagga tgtcggcgtg gccgcagtgc
     1561 tgtgcgaatt cacggag
//
LOCUS       DATBFZ010000192         7204 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_3673631, whole genome shotgun sequence.
ACCESSION   DATBFZ010000192 DATBFZ010000000
VERSION     DATBFZ010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 7204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7204
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3673631"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            176..676
                     /locus_tag="VH419_05820"
     CDS             176..676
                     /locus_tag="VH419_05820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043168.1"
                     /translation="MSGTGWTVLIIVVIVVLALLALAASRRARQQRITRADTMRSEAD
                     ERSVVVDQRAAKAEEVEARARQAQAEADAKSAEAERLQATAAQQQSRVADDRAEVEEQ
                     RTRADELDPRAGETATGEPTGASEYTTEEGETQDTARPGNTGQSDNTAQPDNTGQSDT
                     TGQSPR"
     gene            complement(690..1943)
                     /locus_tag="VH419_05825"
     CDS             complement(690..1943)
                     /locus_tag="VH419_05825"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY7043169.1"
                     /translation="MTGRDLHDREAPQASTPVEVPALGRDRRVQAYVLSSAISQAGDV
                     AWTIGLAWTAARIGTATQTGLVVGIGTFPRALMLLFGGALADRIDARKVMVATNISRI
                     GTLLLGIVVAQGVGLSIPLLVGIAVLFGLFDAVYDPAAGTLPRQFVRTEDLSATVAMF
                     QFAHRVATFVGAPIGGFLVAFGGLELVMFADALSFCVIAAMLAFVMRPRFPRELSSGH
                     SMLKDLIGGFGYLRRTKPVRTLVIALSGLNLCAGPITAVGLVLRTHAAGWGAPSLGVF
                     QACIGVGAAVGAVVAIRVRPSRPARAGLLILIVQSAALSVIGFASYAGIVVAMATVGV
                     TAGLASAFLSGAFMASVDGQYLGRMGSITSLTDNALMPVMMVAFASFAGAVSLTLGCV
                     LLAVAFATLVTWSATRPDIDFAAVG"
     gene            complement(1940..2512)
                     /locus_tag="VH419_05830"
     CDS             complement(1940..2512)
                     /locus_tag="VH419_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012920945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY7043170.1"
                     /translation="MPEILRRRADISKIAAVHHPLRRRLLELLGVHGPATASRLAAET
                     DQLVGNVSHHLKMLSKAGLIEEAPELARDRRERWWRQVPMSLSWSVADMAGNTEDELI
                     AEAAEHQNLSHHVDKVHQWFERRGSYDAEWSRAAYSTDSWLRLTADELVELSEKLGDL
                     VSDFAQTVDVDDGQDRQHVFFFAHAIPAKP"
     gene            2822..3046
                     /locus_tag="VH419_05835"
     CDS             2822..3046
                     /locus_tag="VH419_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043171.1"
                     /translation="MFNVSVRRVVVSAIAIAATSAVSFGASSEAWGGKSVEWGKVGSG
                     HHSAKSVEWGASSVEWGLVKNSGSLSVEWG"
     gene            3088..5835
                     /locus_tag="VH419_05840"
     CDS             3088..5835
                     /locus_tag="VH419_05840"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY7043172.1"
                     /translation="MSSRRPASQRTLALATAATAAAAVGAAAGVVLGLPQTHVPHSNI
                     AAVAIAVCLVALHMIPQRNLTTGSHVENIHLDEVLFVPTVLMLSPWQSLIVVGLGSIA
                     GGLVLRRSTMKNIFNIASMTLSTTAAVLVVHALGVSPTPHPGVGDALAGMLGALTLTS
                     VSALCVRSIVSLASGVSLFLLLKETAVNLAPWAGAVTLGGLGVVAVGAYPASAILVLG
                     VVVFVHRSYAAALNEVNARRQAERLQQAIASLRSHTDPELVKSDLVAAAKELVGADRA
                     DVVTDEDEDPDNAYSAPLPGGQRLRVSDRRGTDHWDDRDRATLVELAGVAGDVLRTAE
                     VIARLRTITNSQSEGVIALDMNAHITFVNPAAVQMLNARRDEDLLGQPIQATLMLRHR
                     RKPIDFPSMVAKQVVAQDADGTLGPSDGETLDIAYSMTPLRAEGAHVGAVLVLRDVTE
                     RRAFQDELTRRALHDELTGLPNRRLLLERFDHAMARSRTSGVSHGLLFLDLDRFKLVN
                     DSYGHLIGDKLLVQVANRLLTGLSPADSVARMSGDEFVILVEDANDIDTMTRVAERLL
                     HILQEPFEVDSHQIFMSASIGVGLTREGQSRDETLAMVDAAAYAAKAAGRNCYCVSTD
                     ASVDETRARLDLEVSLRQGFDENELELHYQPIVTTDGSEVIGVEALVRWRSPRRGMLA
                     PQQFVPIAEETGLIVPMGRWVLEEACRTMREWTLRFPDRRPLSVSVNLSALQFAQHRL
                     PEDVAATLEATGLPASQLCLEITETVLMADTAGTRATLNALHDLGVRVAIDDFGTGYS
                     SLSYLKKFAIDMVKLDRTFMEGLVTDPVDAEIASAVIRLSGALGIRTVAEGVETDSQR
                     RMLVQLGCPLMQGYLTSRPLQADDFTAFWDTNQLTTVLSADDSNVTDLLKHRSLRQQS
                     S"
     gene            5971..6633
                     /locus_tag="VH419_05845"
     CDS             5971..6633
                     /locus_tag="VH419_05845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043173.1"
                     /translation="MYLLRSNPLAGPGSAAVALALCVSLAAPDRVDAAAAGDAPVAPS
                     HVDTTPAVVTTTSVTDPDDVDGRFDIARIEHVVEEVDRDHARISYTVETFAGWADRLL
                     ERRDRNFVLELNRDGEPGSEVNVTVSNRAGHIVADLISNATRRVIRSVPVLRSDDHSF
                     TISGPRDVVGARSYFWTSNLHVRPPHALCGRNRGFPVTCQDSVPERGWIRMNAPAWPR
                     AH"
BASE COUNT         1227 a         2448 c         2426 g         1103 t
ORIGIN      
        1 ggggtttgcg atggttccat cgcaaacccc cgccgcacac cgcagacctg cgatgcaaag
       61 acaggagccg ggctgtaaac atcgcaaacc caccagcgac gtacgaaatg gacgaggtcg
      121 aggtttgcgg ggcacggcga cgggcacttg ggttccggac cagaggaggc ccaagatgtc
      181 cggaaccgga tggaccgtcc tgatcattgt cgtcatcgtc gtgctcgcac tgctggcgct
      241 cgctgccagc cgtcgggctc ggcagcagcg gatcacccgc gccgacacca tgcgttcgga
      301 ggccgacgag cggtcagtcg tcgtggacca gcgggccgcc aaggccgaag aggtcgaggc
      361 acgggcgcgg caggcccagg ccgaagccga tgccaagtcc gcggaggcag aacgcctgca
      421 ggcgactgcc gcccaacaac agtcgcgcgt ggccgacgac cgcgccgagg tcgaagagca
      481 gcggacccgc gccgacgagc tcgacccgcg cgccggcgag acggcgactg gagagccgac
      541 gggtgcttcg gaatacacga ccgaggaggg ggagacgcag gacaccgccc gtcctggcaa
      601 cacagggcag tctgacaaca ccgcgcagcc tgacaacacc gggcagtctg acaccaccgg
      661 gcagtctccg cgctgagaga ctcagcgggt cagccgacag cggcgaagtc gatgtcgggg
      721 cgggtggccg accaggtcac caacgtggcg aacgcgaccg ccaacagcac gcaccccaag
      781 gtgaggctca ccgccccggc gaaggaagcg aacgcgacca tcatgaccgg catgagggcg
      841 ttgtcggtga gtgaggtgat cgagcccatc cgccccaggt actgaccgtc cacgctggcc
      901 atgaacgcgc cggacaggaa cgccgacgca agaccggcag tgacgcccac ggtcgccatc
      961 gcgaccacga tcccggcgta cgacgcgaac ccgatcacgc tgagtgcagc cgactgcacg
     1021 atgagaatca gcagcccggc tcgagccggc cgcgacgggc gcacccggat cgcgacgacg
     1081 gctcccaccg cggcgcccac gccgatacag gcttggaaga ccccgaggct gggggcaccc
     1141 cagccggctg cgtgggtccg aagcacgagt ccgacagccg tgatggggcc ggcgcagagg
     1201 ttgaggccgg acaaggcgat gacgagggtg cgcaccggct tggtgcggcg caggtacccg
     1261 aagccaccga tgaggtcctt cagcatcgag tgaccgctgg acagctcgcg cgggaaacgc
     1321 ggccgcatga cgaatgccag catggcagcg atcacgcaga aactgagcgc gtcggcgaac
     1381 atgacgagct cgagcccgcc gaacgccacc aggaaaccgc cgataggtgc cccgacgaag
     1441 gtggcgaccc ggtgggcgaa ctggaacatc gcaacggtcg ccgacaggtc ctcggtccgc
     1501 acgaactggc ggggcagagt gcccgccgcc gggtcgtaga cggcatcgaa cagcccgaac
     1561 agtacggcga tgccgaccag caaggggatg gaaaggccca ctccctgggc cacgacgatg
     1621 cccaacagca gcgtgccgat gcggctgatg ttcgtcgcca ccatgacctt ccgcgcgtcg
     1681 atacgatcgg caagggcacc gccgaagagc agcatcagtg cgcgcgggaa cgtgccgatg
     1741 ccgacgacga ggccggtctg ggtggcagtt ccgatcctcg ccgcggtcca ggcaagcccg
     1801 atcgtccacg ccacgtcacc ggcttgcgag atggcgctcg acaagacgta cgcctgcacg
     1861 cgccggtccc ggcccagcgc aggcacttcg accggcgtac tcgcctgtgg tgcttcacgg
     1921 tcgtgcagat ctcgaccggt catggcttcg ccgggatcgc gtgcgcgaag aagaacacgt
     1981 gttgtcggtc ttggccgtcg tccacgtcga cggtctgcgc gaagtccgac acgaggtcac
     2041 cgagcttctc gctgagctcg acgagctcat cggcggtgag ccgcagccaa gagtccgtgg
     2101 agtacgccgc ccgggaccac tcggcgtcgt agctgccacg gcgctcgaac cactgatgca
     2161 ccttgtcgac atgatgtgac aggttctggt gctctgcggc ttcggcgatc agctcgtcct
     2221 cggtgttgcc ggccatgtcc gccaccgacc agctgagcga catcgggacc tgccgccacc
     2281 accgctcgcg gcggtcgcgg gcaagctccg gcgcctcctc gatcagaccg gccttcgaca
     2341 gcatcttgag atggtggctg acgttcccca cgagctgatc ggtctcagcc gcaagccgcg
     2401 aggcggtcgc gggtccgtgc acccccagca gctccaggag tcgccggcga agcggatggt
     2461 ggaccgcggc gatcttggag atgtcggcgc gacgtcgaag gatctccggc atggggccga
     2521 ccatatatgt acaacgcctc ttgtgcaaga cgtcttgtgt atggcgcacc cgatgaagga
     2581 gggtcccccg acgggtggcc gatttgtcct gggatgcagg ggaaaacccc atgcatcgtc
     2641 cctgtggacg ggcagatgct gccgagttcg gggacgtatc gccgtaggac cagaagttac
     2701 cgggctgcgg ccaaggctat tttgacgacc cggacaggtt ttcgcggtca actgctgcca
     2761 tgctatgagg gctcgcgcga cgcggtgtcg cgcgcacgtc ctcacagcaa ggatccccct
     2821 catgttcaac gtctccgtgc gtagggttgt cgtctccgcc atcgccatcg ccgccacaag
     2881 tgctgtctca ttcggcgcct cttccgaggc ctggggcggc aagtccgtgg agtgggggaa
     2941 ggtcggctcc ggccaccaca gcgccaagtc cgtcgagtgg ggcgccagct ccgtcgagtg
     3001 gggcctggtc aagaacagcg gcagcctttc ggtcgaatgg ggctgagccg acacggtctc
     3061 cacgggcctg gtagcgggat gaccgcggtg tcatctcgtc gccccgcgtc ccagcggacg
     3121 ctcgcgttgg cgaccgctgc cacggcagcg gcagcggtcg gtgctgcagc cggtgtcgtc
     3181 ctcgggctgc cgcagacgca tgtgccgcac agcaacatcg cagctgtcgc gatcgcggtc
     3241 tgcctcgttg cgctgcacat gattccgcag cgcaacctga cgaccggaag tcacgtcgag
     3301 aacatccacc tcgacgaggt gttgttcgtg ccgacggtgt tgatgctttc gccctggcag
     3361 agcctcatcg tggtcgggct cggatccatc gccggcgggc tggtgcttcg gcgctccacg
     3421 atgaagaaca tcttcaacat cgcctcgatg acgctgtcca cgacggcagc agtgttggtc
     3481 gtgcacgcgc tcggcgtcag cccgacgcct catccgggtg tcggtgacgc tcttgccggg
     3541 atgctcggcg cgttgacgct gaccagcgtg tccgcgctct gcgtacgctc catcgtctcg
     3601 ctggcgagcg gcgtttcgct gttcctgctg ctcaaggaaa cggctgtcaa ccttgcgcca
     3661 tgggccggcg cggtgaccct cggcggcctc ggtgtcgtcg cggtcggcgc ataccctgcc
     3721 tcagcgatcc tggtgctcgg cgtcgtggtg ttcgtgcatc gctcgtacgc cgccgcgctc
     3781 aacgaggtca acgcgaggcg acaggcggag cggctgcagc aggcgattgc atcgcttcgc
     3841 agccacaccg atccagagct ggtcaagagc gatctcgtcg ctgccgccaa ggagctcgtc
     3901 ggggccgacc gggcggacgt cgtcacggac gaggacgagg acccggacaa cgcctacagc
     3961 gctcccctgc ccggcggcca gcggctgcgc gtgagcgacc gtcgcggcac ggaccactgg
     4021 gacgaccggg acagggccac gctggtggag ctggccgggg tggccggtga cgtcctgcgc
     4081 acggcagagg tcatcgcgcg gctgcgcacg atcacgaact cgcagagtga gggcgtcatc
     4141 gccctcgaca tgaacgcgca catcacgttc gtcaacccgg ccgccgtcca gatgctcaac
     4201 gcccgccgtg acgaggatct gctcggacag ccgatccagg ccacgctcat gctgcggcac
     4261 cgccgcaagc ccatcgactt ccccagcatg gtcgccaaac aggtggtcgc gcaggacgcc
     4321 gacggcaccc tcgggccgtc cgacggcgag acgctcgaca tcgcgtacag catgacgccg
     4381 ctgcgcgccg agggcgcaca cgtcggcgct gtgctcgtgc tccgtgacgt gaccgagcga
     4441 cgtgcgttcc aggacgagct gacccgacga gccctgcacg acgagctgac cggcctgccg
     4501 aaccggcggc tgctgctgga gcgcttcgac catgcgatgg cgcgatcgag gacgtcaggc
     4561 gtcagccacg gcctgctctt cctcgatctc gaccggttca agctggtcaa cgactcctac
     4621 ggccacctga tcggcgacaa gctgctggtc caggtcgcca accggctgct gaccggactg
     4681 tcaccggcgg actcggtggc ccggatgtcc ggtgacgagt tcgtcatcct ggtcgaggac
     4741 gccaacgaca tcgacaccat gacgagggtc gccgagcggc tgctccacat cctgcaggaa
     4801 ccgttcgagg tcgactcgca ccagatcttc atgtccgcgt cgatcggcgt cggcctcacg
     4861 cgtgagggac aaagccgcga cgagacgctg gccatggtgg acgctgcggc gtacgccgcc
     4921 aaggccgccg gtcgcaactg ctactgcgtc tccaccgacg cgtccgtcga cgagacgcgc
     4981 gctcgactcg atctcgaggt gagcctgcga cagggtttcg acgagaacga gctcgagctg
     5041 cactaccagc cgatcgtgac gaccgatggc tccgaggtca tcggcgtgga agcactcgtg
     5101 cgttggcggt cgccgaggcg cgggatgctc gctccgcagc agttcgtgcc gatcgcggag
     5161 gagaccgggc tcatcgttcc gatgggccgc tgggtactcg aagaagcgtg ccggaccatg
     5221 cgcgagtgga cgctgcgctt ccccgaccgt cgtccgctga gcgtttccgt caacctctct
     5281 gcactgcagt tcgcgcaaca ccggctgccg gaggacgtag ccgccaccct cgaggccacc
     5341 gggctgccgg cgtcgcagct gtgcctcgag atcaccgaga cggtcctgat ggcggacacc
     5401 gccggaacgc gggcgacgct gaacgcgctg cacgacctcg gcgtacgcgt ggcgatcgac
     5461 gacttcggca ccgggtactc gtcgctgtcc tacctcaaga agttcgcgat cgacatggtc
     5521 aagctcgacc gaacgttcat ggagggcctc gtcaccgacc ccgtcgacgc cgagatcgcg
     5581 agcgccgtga tcaggttgtc gggcgcgctc ggcatcagga cggtggcaga aggagtcgag
     5641 accgactcac agcggcggat gctggtacag ctcggctgcc cgctcatgca gggctacctg
     5701 acgtctcgcc ctctgcaggc cgacgacttc actgcattct gggacacgaa ccagctcacg
     5761 accgtgctga gcgcggacga ctccaacgtc accgacctgc tcaagcaccg ctcactgcgg
     5821 caacagagca gctgaccgcg gaatccacat acagagggcc gatggcgcac tgtccacagc
     5881 atgctgttgt gtgccaaccc gctgaccgcg actcgcccta gcgtcgggcc gctgccgacc
     5941 ggcagtgacc ctgagacgaa cggcgagccc atgtacctgc ttcgatccaa tccgttggcc
     6001 ggacctggct cggccgccgt cgcactggca ttgtgcgttt ccctcgcagc cccggaccga
     6061 gttgacgccg cggccgccgg cgacgccccc gtggcgccgt ctcatgtcga cacgacgccg
     6121 gccgtggtga cgacgaccag cgtcaccgac cccgacgatg tcgacggacg gttcgacatc
     6181 gcccggatcg aacatgtcgt cgaggaggta gaccgcgacc acgcccggat cagctacacc
     6241 gtcgagacgt tcgccggctg ggccgatcga ttgctggaac gtcgagatcg caacttcgtg
     6301 ctcgagctga accgagacgg tgagccaggc tcggaggtga acgtgaccgt gtcgaaccgt
     6361 gccggccaca tcgtcgcgga cctcatctcg aacgcgacca ggagagtgat ccggtccgtt
     6421 cccgtactcc ggtccgacga ccactcgttc acgatcagcg gcccccgcga cgtcgtcgga
     6481 gcgcgttcat acttctggac gagcaacctc cacgtgcgac ccccgcacgc cctgtgcggc
     6541 cgaaaccgcg ggttcccggt cacctgccag gactcggtac ccgagcgggg ctggatccgg
     6601 atgaacgcgc cggcgtggcc acgtgcccac tgatggggtc ctcaggttcg tactaggggg
     6661 gcaggcttgc gatgcgtgtt cgtggtcacc cgacgcacca tcgtcctggg cggttaagaa
     6721 gatcggcagc gaagcgcagg aagcattcct gctcgttggc ctggtcgacc ggcttcctgc
     6781 cggcgttcat ggtcgcccgg ggtggcgtgc tctccgcggc ggccaccgtc ggcgccgggt
     6841 gaggtggtcc gtgctcgtcg gacgcgtcgg ccggcgtgac gtcctttcgg cttggtcacg
     6901 agtggcggtt ttcggacttc tggcgccgcg gccgatctgc cgcgtccccg tgaccagtcc
     6961 cacaggggct cttctgcggc tcctgcgatt tcagtgggcg gagacggtca cgacgggtgg
     7021 acgcggtcgc ccgtcgtccc gtcgtcctgg gctgaggtgg ctcggcaccg aacagccggc
     7081 gacgttcgtg cccgcggagc tggtcggccg gctttccgtc ccgacggacg gcctaggagt
     7141 cgcaggtctc ggacatccgc aacggagcct cgaggtttgc gatgttcaca tccgagaccc
     7201 actg
//
LOCUS       DATBFZ010000193        12244 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_456999, whole genome shotgun sequence.
ACCESSION   DATBFZ010000193 DATBFZ010000000
VERSION     DATBFZ010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 12244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12244
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456999"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(134..475)
                     /locus_tag="VH419_05850"
     CDS             complement(134..475)
                     /locus_tag="VH419_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020578569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043174.1"
                     /translation="MDFIRDLLLFIHLLGFAALLGGALVQITDETHVVNSAMLHGILT
                     QVVSGLLLVGVLEGQDEPVDHTKMAVKLGIALVIAVLCWVNRRKEDVPAGLFMGILLL
                     TVTNAAIAVFW"
     gene            complement(489..773)
                     /locus_tag="VH419_05855"
     CDS             complement(489..773)
                     /locus_tag="VH419_05855"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY7043175.1"
                     /translation="MDEVPTIVDVPKSHRYELVVDGKQVGRLEYAVRGDTFIAIHTEV
                     DPAYAGHGLAEQLVSHVLDEVRDTGMSLRPLCPYVKRFLEKHPEYDDLVS"
     gene            complement(773..1435)
                     /locus_tag="VH419_05860"
     CDS             complement(773..1435)
                     /locus_tag="VH419_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY7043176.1"
                     /translation="MSLSNARTGPLRIVIVDDHAMFRTGVKAELGPTVQVVAEAADVE
                     EAVAAIEAERPDVVLLDVHLPGGGGTEVLRRTTTRHPDVRFLALSVSDAAEDVIAVIR
                     GGARGYVTKNITGPELVAAIRRVSEGDAVFSPRLAGFVLDAFAGQIDVGDVDEDLDRL
                     TEREREVMRLIARGYAYKEVASTLFISIKTVETHVSSVLRKLQLSSRHELTRWATERR
                     LL"
     gene            complement(1438..2715)
                     /locus_tag="VH419_05865"
     CDS             complement(1438..2715)
                     /locus_tag="VH419_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017934630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEY7043177.1"
                     /translation="MSTAGAPGRHTVLEPRRLFRDPEDRMLGGVAAGVARHLGVSTTL
                     VRVAFLVMVLWSGFGAVLYAALWALVPLDDPSIDETAAPGLASAQRRGLRATRSIGPR
                     DVGVAASIGVLAFGGIVLLHNFGLWVSPHLFWPLLAAAIGMALLWRQADERDRQEWLS
                     SDGGWKAWLRILAGVALLIGAGGLALYEAGVSGQGSVTDILGAILLAVVGFGLVLGPW
                     ALRLNNDLRRERTERIRSQERADVAAHLHDSVLQTLALIQRQAGDPAAVNQLARTQER
                     ELRTWLFESPSMPEDTVKAALQQTVADVELAHHVPIEMVAVGDRPLDTRMSAVVAAAR
                     EAMVNAAKHSGAARVDVFLEAGESELEVFVRDRGSGFDQDDVPEDRLGVRRSIIDRMQ
                     RHGGHAEVRSGPEGGTEVRLAMPVSYASNGQEG"
     gene            2889..4304
                     /locus_tag="VH419_05870"
     CDS             2889..4304
                     /locus_tag="VH419_05870"
                     /inference="COORDINATES: protein motif:HMM:NF015957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEY7043178.1"
                     /translation="MTSAPPPATSGPRVTRDEVRDLSRLRRSRQPRMLAGVCEGISRH
                     LDIDPLLVRVIFGALTVFGGAGIVLYVIAWLTIPEEGATDSVASRAFHRDADRTMVLG
                     LSLAAIAAAVTLLSAIGFATPRPWAVITVSLVALVLFALFSRRAGGHPSYPTTPPYPT
                     PQSPTGPAQPAYDAADDGVGDDTAHDDTTATTTATAVIPTVTPEEREAEMKAWWQRST
                     PPGGPVLPPTPAPPPPPRKEPRPRSRLTPITLAVMVVTLGGIWLADATGADIHPSVYP
                     GSVLALTAVALIVGTWFGRAKLLIPVGILAALLTAAFTVIGPGPYGERIYNPSTASAV
                     ESTYDHGAGRLVVNLSYVDDVDALDGRTIDVDGHVGLIQVVIPTTLDADVSAHVDAGD
                     IRGPATAEDTGNGSEEAVMSARHDDRPIVTIDVDLRFGRIEILRFDCPGLAIAQRGTT
                     DSQDLSTLSWEGSDRDPAACH"
     gene            4282..4512
                     /locus_tag="VH419_05875"
     CDS             4282..4512
                     /locus_tag="VH419_05875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043179.1"
                     /translation="MIPRHATDIVSLVAGTIFAGCTIIWILAVTDTIDATQAWIGWPL
                     TMIAAGVVGLVAALRPNRQPSPTSESSDPISS"
     gene            4594..6294
                     /locus_tag="VH419_05880"
     CDS             4594..6294
                     /locus_tag="VH419_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008364082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY7043180.1"
                     /translation="MRRLPLADPGIPDVRSPVRYLLWVARGQLWTVFWGVTFGTVWMV
                     AQSVMPAIIGRAIDEGVRADDMSRLWMWALVLLGVGLLQAGTGILRHRYAVQNWLIAA
                     YRTVQVVSRHAVRLGATLPRRVSTGEVVSIGSSDLAHVGNSIEVMGRAAGAVVSFFVV
                     AAILLHASTALGLLVLIGVPLLLLLLGPILKPLQRRNLAQREMMGELNTLASDIVGGL
                     RVLRGIGGEDVFHRRYVEESQKVRAAGVQVARLQSLLDGLQVFLPGLFVVIVVWVGAR
                     FAVEGEISPGELVAFYGYSAFLMIPLRTGTEVANKVIRGLVASRRICGVIAVEPEAQD
                     IAEPKTASTADELVDVRSGLHVRPGQLTAIVAEVPEDSAAVADRLGRFADGPALLGGV
                     SLSELERDFVRQAILLSDSNATLFSGRLRDELDVRGHASDDDILTAIGTASAEDVIEA
                     LDDGLESTVEERGRSFSGGQRQRLVLTRALVADPDILVLVEPTSAVDAHTEARIAERL
                     RRHRSGRTTVVTTASPLMLDHADEVALLDDGRVVAVGRHRDLLDDPRYRRIVARGEDS
                     "
     gene            6291..8060
                     /locus_tag="VH419_05885"
     CDS             6291..8060
                     /locus_tag="VH419_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012922870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY7043181.1"
                     /translation="MKQLLPVADGAAVRRYTVRLARRHPRPLAVAVSLHVLAAVAGLA
                     APRLIGNLVQDVSTGTTVSAVDKTVAAIAVFLLAQSILTRYARFCSHKLGEQVLAELR
                     EDFVKQSLALPIGTVEKAGTGDLLTRSSRDVEALGWSVRFAMPETIIAFVTTVFTIAA
                     CILVGTWVLVPLVLGVPLLFVATRWYLKRAKPGYLRESASYAAINASVAETTDGARTV
                     EALRLEKRRLERIDADIAESFAAERYTLFLRTVWFPAVEVGYLVPTVVTLLFGGYLHS
                     QGQVDIGDVTAATLYVQALIDPVDRLISWLDELQVGAASLARLLGVADVPDDRTATGR
                     TPAHQDIEAEDVRFAYVDSRDVLHGVDLKVSRGERVAMVGPSGAGKSTLGRLLAGIHP
                     PRTGSVTIGDVGMTELPLGELRGEVALVTQEHHVFVGTVRDNLALAVDDLEGEVGHDR
                     LVAALRAVDALDWVMALPDGLETHVGSGGLSLPPSQAQQLALARLVLADPHTLVLDEA
                     TSLIDPRSARHLERSLAAVLHGRTVIAIAHRLFTAHDADRVAVVEDGRVSEIGSHHEL
                     IDAAGSYAALWQSWHGEHESATA"
     gene            8077..8733
                     /locus_tag="VH419_05890"
     CDS             8077..8733
                     /locus_tag="VH419_05890"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HEY7043182.1"
                     /translation="MKRFLLQTFAELDAQRPAGCPELVHFTESLATTVIEEYSNPGDL
                     VLDPFAGFGTTMVVAERLGRRGYGVELLQERVNQISASLRDSGSIVLGDARNLAQLVR
                     SLRGSVSLVLTSPPYMSRNDHPENPLDAYATMTGDYGTYLEEIAEVFADAATLLRPDG
                     HVVVNVANIVHDGVLTPLAWDMARAISARLTLVQECYLAWDSQPADISGDYCLVFRQG
                     "
     gene            8888..11008
                     /locus_tag="VH419_05895"
     CDS             8888..11008
                     /locus_tag="VH419_05895"
                     /inference="COORDINATES: protein motif:HMM:NF019325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HEY7043183.1"
                     /translation="MTASRHRGILRHRSVFSLATAIGTFGLLTNGLVSTPALAAGRPD
                     LQVTTVTDSPGARAPGQHFTIKVTVRNGGRSRSPRTSTAFYLSKDARKSSGDVALIGR
                     AVVPALAPGKRAAVAKAVTIPRTTPSALYRVIACADASHGVRETHEGNNCRAATPVLS
                     RVLVSPPLPHPDPLDVSTTAEPDATASAYVVPGSASTITATGSDGTTFALAIPAHAVA
                     HPITVSLTPIASIVDNPLSGGLGAAAELGPSGLQLLEPATLRITPGDPSILTPPAGEQ
                     LDGFAFSGAGREFRLFPDLGSLPGASVPDGQMWFAVQHFSGTGFGLGTAADETSVEGH
                     IPVAPEPTFEAAMATIVNGVKRGDIPQQELGPRLGDALVEDYLFHVIPALNAGLAANA
                     EPGDGEAAKDVFLHWAHWVAILGYEGGTNAKYKITYGALLAAGDDAIAEMLSKFFNQA
                     YQRCIKHVQPKRQYLLMWGFARSASVLGDPDDILGKGWQRSIKDCEDNFPTTWEGTIT
                     YTWHIDAPTPCGGTCIADSQTGTGTVHAAFEPGGSATGWTLSAGNVNVSYVRSSTDLG
                     TDGVCQHTSTTAWTYSGASTSDHMMLNNQPSNAFVYFDFSGFPTVPTHTVFTTNCPHE
                     GGYEDTSPMGQQLAPLVWCPEYPRHNPDGSTDGTSGWIRGTRNSVGGQQVLDFTCTDS
                     YDRGTGTSLRHWSVQTSGVFTKPS"
     gene            11116..11826
                     /locus_tag="VH419_05900"
     CDS             11116..11826
                     /locus_tag="VH419_05900"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEY7043184.1"
                     /translation="MFVDVATDRVRDGATPDRSWTRTAVLVVVWWVAIATIVISVGWL
                     ITHPFLDVVGPRDDDVVRWFADQRTPFLDDVAEYGTWGGETVVTLVVAPILAIGCWVL
                     TRSVVPALFVLLVICGVGGFYWAGTMLVPRRRPPVHILDPGLVPTDSFPSGHVGTAVA
                     LYGAVVVLTWTYARSRRWFVTPLLVMPVFVLLSRLYQGAHHLTDVLTSLMYGFTWLAV
                     VAALVLRGHHQAAKVPMR"
     gene            complement(11837..>12244)
                     /locus_tag="VH419_05905"
     CDS             complement(11837..>12244)
                     /locus_tag="VH419_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017936373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease-associated protein"
                     /protein_id="HEY7043185.1"
                     /translation="IGFITYPANVNALTALVSFGVSGIYLSFLLTVIAAIIARSRGWV
                     PAGRFQLGKWGWPVCIAAALYLGLMLLNVVAPTGLSSPRAYFNLDWITLLVIAVIAIV
                     GALYFFLARPDRGVQRHLHDTLEPTGAEREHPA"
BASE COUNT         1876 a         4284 c         4097 g         1987 t
ORIGIN      
        1 gtcgagatcg aggggtttcg acaagctcaa ccaccggagg cccgctgatc gagcctgtcg
       61 agtagcccgc tgatcgagcc tgtcgagatc gaggggtttg gacaagctca accaccgaac
      121 tcgggtccgt ccatcaccag aagaccgcga ttgcggcgtt ggtgacggtg agcagcagga
      181 tgcccatgaa gagaccggcc ggcacgtcct ccttgcggcg gttcacccag cacagcacgg
      241 cgatgaccag cgcgatgccg agcttgaccg ccatcttcgt atggtcgacc ggctcgtcct
      301 gaccctcgag tacgccgacc agcagcagtc cgctgaccac ctgcgtcaag atcccgtgca
      361 gcatggcgga gttcacgacg tgcgtctcgt cggtgatctg gacgagggca ccgccgagca
      421 gggcagcaaa accgagcagg tggatgaaca gcagcaggtc tcggatgaag tccacaggcg
      481 cactcttctc agctgaccag gtcgtcgtac tcgggatgct tctcgaggaa ccgtttcacg
      541 tacgggcaga gtggtcgcag cgacatgccg gtgtcgcgga cttcgtcgag gacgtggctg
      601 accagctgct cggcaagacc gtggccggcg tacgccgggt cgacctcggt gtggatggcg
      661 atgaacgtgt cgccgcgaac ggcgtactcg agccgcccga cctgcttccc gtcgaccacc
      721 agctcgtagc ggtggctctt cggtacgtcg acgatggtcg ggacttcgtc catcagagca
      781 accgccgctc ggtcgcccac cgggtcagct cgtgccgtga cgagagctgc agcttgcgca
      841 gcacgctcga gacgtgcgtc tccactgtct tgatcgagat gaacagcgtc gaggccacct
      901 ccttgtacgc gtagccgcgg gcgatcagcc gcatgacctc ccgctcccgt tcggtcagcc
      961 ggtcgaggtc ctcgtcgacg tcgccgacgt cgatctgccc cgcgaacgcg tcgaggacga
     1021 agccggccag ccgtggcgag aagaccgcgt ctccctcgct caccctgcgg atcgcggcca
     1081 ccagttccgg accggtgatg ttcttcgtga cgtagccccg cgcgccaccg cggatgaccg
     1141 cgatcacgtc ctccgcggcg tcggaaacgc tcagcgccag gaaacgcacg tccggatggc
     1201 gcgtggtcgt acgacggagc acctcggtgc cgccgccgcc cgggagatgg acgtcgagca
     1261 gcacgacgtc cggtcgctcc gcctcgatgg ccgccactgc ctcctcgacg tcggccgcct
     1321 ccgcgacgac ctggacggtc ggccccagct cggccttgac gccggtacga aacatcgcgt
     1381 ggtcgtcgac gatcacgatt cgaagcggtc cggttctggc gttcgacagt gacatgctca
     1441 gccttcctgg ccgttggatg cgtacgagac cggcatggcg agacgtacct ctgtcccgcc
     1501 ctccgggcca cttcggacct cggcatgtcc gccgtgccgt tgcatccggt cgatgatcga
     1561 gcggcgtacg ccgagccggt cctcgggtac gtcgtcctgg tcgaaaccgg agccgcggtc
     1621 ccggacgaag acctccagct ccgactcacc cgcctcgagg aagacgtcga cgcgcgcagc
     1681 gcccgagtgc ttggccgcgt tcaccatcgc ctcgcgggcg gcagccacga cggccgacat
     1741 cctcgtgtcc aggggccggt cgccgaccgc caccatctcg atcgggacat ggtgggcgag
     1801 ctcgacgtcg gcgacggttt gctggagggc ggccttcacc gtgtcctccg gcatggaagg
     1861 ggactcgaac agccacgtcc ggagctcgcg ttcctgcgtc cgcgcgagct ggttcaccgc
     1921 cgcggggtca cccgcctgcc gctggatcag ggccagcgtc tgcagcactg agtcgtgcag
     1981 gtgcgccgcg acgtcggccc gctcctggga ccggatgcgc tccgtgcgct cgcgacgcag
     2041 gtcgttgttg agccgcagtg cccacgggcc gaggacgaga ccgaagccca ccacggcgag
     2101 caggatggcc ccgaggatgt cggtcacgct tccctgcccg gacacacccg cctcgtacaa
     2161 cgccaggccg ccggcgccga tcaacagcgc caccccggcg aggatccgca accacgcctt
     2221 ccacccgcca tcgctgctca accactcctg gcggtcgcgt tcgtcggcct gcctccacag
     2281 cagcgccatg ccgatcgcag cggccagcag tggccagaac aggtgtgggc tcacccagag
     2341 gccgaagttg tgcagcaaca cgatgccccc gaacgcgagg acgccgatgg acgctgccac
     2401 accgacgtca cggggcccga tcgacctggt tgcccgcagc cctcgtcgct gtgcagacgc
     2461 cagcccgggc gccgctgtct cgtcgatgga cgggtcgtcg agcgggacga gcgcccagag
     2521 ggcggcatac aggaccgccc cgaacccgct ccacagcacc atcacgagga aggcgacgcg
     2581 gaccaacgtc gtcgagacgc ccaggtgcct cgcgacaccg gccgcgacgc cgccgagcat
     2641 ccggtcttcc gggtcgcgga agagccgtcg tggttcgagg accgtgtgac gtcccggcgc
     2701 tccagcggtg gacatgcctc catgattgcg gcaccaccgg cccgagacca tccgcgtctc
     2761 ccctgatcga tcccttgccg gttcgcacgc agcgtcaggg gcggtcaggg gcgcgtcagg
     2821 gtccgcgcgg caagatcggg gccttccccg attccgcatc atgccggtca tgccggaagg
     2881 ttgacgtcat gacaagtgca ccaccaccgg ccacgtccgg accgcgcgtc acccgcgacg
     2941 aggtccgtga cctctcgcgg cttcgccgca gccgccagcc gcgcatgctc gcgggtgtct
     3001 gcgaaggcat cagccgtcac ctcgacatcg acccgctgct cgtccgggtg atcttcgggg
     3061 cgctcaccgt gttcggcggc gcgggcatcg tcttgtacgt catcgcctgg ttgacgatcc
     3121 cggaggaggg agcgaccgac tctgtcgcgt cccgggcatt ccatcgagac gcggaccgga
     3181 ccatggtcct cgggctctca ctggctgcga tcgccgccgc tgtcaccttg ctcagcgcga
     3241 tcggcttcgc gacaccgcga ccgtgggcgg tcatcaccgt cagcctggtg gcgctggtcc
     3301 tgttcgcgct gttcagccgc cgtgccggcg ggcatccgtc gtacccgacg actccgccct
     3361 atcccacgcc gcagtccccg acgggaccgg cgcaaccggc gtacgacgcg gccgacgacg
     3421 gcgtaggcga cgacaccgca cacgacgaca ccactgcgac gacgaccgcc accgccgtga
     3481 tcccgactgt gacaccggaa gagcgggaag cggagatgaa ggcgtggtgg cagcggtcca
     3541 ccccgcccgg cggaccggtt ctgccgccga ctcccgcacc gccgcctccg ccgcgcaagg
     3601 aaccgcgacc gcggtcacgg ctcacgccga tcaccctggc cgtgatggtc gtgacactcg
     3661 gcggcatctg gctcgccgac gccaccggcg ccgatatcca cccgtccgtc tatcccggca
     3721 gtgtgttggc gctcaccgca gtcgcactga tcgtcgggac ctggttcggc cgcgcgaagc
     3781 tgctgattcc cgtcggcatc ctcgccgcgc tgctcaccgc cgccttcacc gtgatcggcc
     3841 caggccccta cggtgagcgc atctacaacc cgtccacggc gtcggcggtc gagtccacct
     3901 acgatcacgg cgccggtcgg ctggtcgtga acctgtcgta cgtcgacgac gtggacgcac
     3961 tcgacgggcg caccatcgat gtggacggtc acgtcggcct gatccaggtt gtcatcccga
     4021 ccacgctgga cgccgacgtc tctgcacacg tcgacgccgg agacatccgc ggacctgcca
     4081 cggcggagga caccggcaac ggttccgagg aagcggtgat gtctgcgagg cacgacgaca
     4141 ggccgatcgt cacgatcgac gtcgacctca ggttcggccg gatcgagatc ctgcgcttcg
     4201 actgtccggg actcgcgatc gcccaacggg gcacgaccga tagccaagac ctgagtacgt
     4261 tgagctggga agggagtgac cgtgatcccg cggcatgcca ctgacatcgt ctcgttggtg
     4321 gccggcacca tcttcgccgg ttgcaccatc atctggatcc tcgccgtgac ggacacgatc
     4381 gacgcgacgc aggcgtggat cggctggcca ctcacgatga tcgccgccgg tgtcgtcggc
     4441 ttggtggcgg cgcttcgacc caaccgccaa ccgtctccca cctcggaatc gtccgacccg
     4501 atctcgtcgt aaggctgcgg gcgcacctcg aggcttcgtg cggcagtaaa tcgatggcgg
     4561 cgcggagtcg ccgatcccta ccgttggatg gtcatgcgcc gccttcccct cgcggatcct
     4621 gggatccccg atgtccgctc gcctgtgcgc tacctgttgt gggtggcccg cgggcaactg
     4681 tggactgtct tctggggtgt gacgttcggc accgtgtgga tggtggcgca gtcggtcatg
     4741 ccggcgatca tcggtcgggc gatcgatgag ggcgttcgcg ccgacgacat gagccggctg
     4801 tggatgtggg cactggtcct gctcggagtt ggcctcttgc aggccgggac cggcatcctg
     4861 cggcaccggt acgccgtgca gaactggctg atcgcggcgt accggaccgt gcaggtcgtc
     4921 tcccggcacg ccgtgcggct cggtgccacg ctgccgagac gcgtctcgac cggcgaggtc
     4981 gtctcgatcg gctcgtccga tctcgcgcat gtcggaaact cgatcgaggt catgggtcgg
     5041 gcggccggcg cggtcgtctc gttcttcgtc gtcgccgcga tcctgctgca tgcgtcgacg
     5101 gcgctcggac tgctggtgct catcggcgta ccgctcctgc tgctcctgct cgggccgatc
     5161 ctcaagccgc tgcagcgccg caacctggcg cagcgggaga tgatgggcga gctcaacacg
     5221 ctcgcttccg acatcgtcgg cggtctacgg gtactccgtg gcatcggcgg tgaggacgtg
     5281 ttccaccgtc gctacgtcga agagtcccag aaggtgcggg ccgccggagt ccaggtcgcc
     5341 cgcctccagt cgctgctcga cggcttgcag gtgttcctgc cggggctgtt cgtcgtgatc
     5401 gtcgtgtggg tcggcgcgag gttcgccgtc gaaggtgaga tctcaccggg cgagctggtg
     5461 gcgttctacg ggtactcggc gttcctcatg atcccgctgc gcaccggtac cgaggttgcc
     5521 aacaaggtga tccgcgggct cgtggccagt cgccggatct gcggggtgat cgctgtcgag
     5581 cccgaggcgc aggacatcgc agagccgaag acggcctcca cggccgacga gctcgtcgac
     5641 gtccgttcgg gcctgcacgt ccggccgggc cagctgacgg cgatcgtggc cgaggtgccc
     5701 gaggactctg cagccgtcgc cgaccggctc ggccggttcg cggacgggcc ggcgctgctc
     5761 ggcggcgtgt cattgtcgga gctcgagcgt gacttcgtcc ggcaggcgat cctgctgagc
     5821 gacagcaacg ccacgctgtt cagcgggcga ctgcgcgatg agctcgacgt ccgcggtcac
     5881 gcgtccgatg acgacatcct cacggccatc ggcacggcgt cggcggagga cgtgatcgag
     5941 gcgctcgacg acgggctcga gagcaccgtc gaggagcgag gccggtcgtt ctccggcgga
     6001 cagcggcagc ggctcgtgct gacccgcgcc ctggtggcgg acccggacat cctcgtgctg
     6061 gtcgagccga cgtcggccgt cgacgcccac acggaggcac ggatcgccga gcggctgcgg
     6121 cgacacagga gcggccgcac gaccgtagtc accaccgcga gcccgctgat gctcgaccat
     6181 gcggacgagg tcgccctgct cgacgacggc cgggtggtcg cggtcggtcg gcatcgcgac
     6241 ctgttggacg acccgaggta ccgacggatc gtggcgagag gggaggactc atgaagcagc
     6301 tgctgcccgt tgccgacggg gccgccgtgc gccgctacac cgtgcgcctc gcccgtcgtc
     6361 atccgcgtcc gctcgccgtt gccgtttccc tgcacgtact cgccgccgtc gcaggactcg
     6421 ccgcaccgcg gttgatcggc aacctcgtcc aggacgtctc gaccggcacg accgtctcgg
     6481 cggtggacaa gaccgtcgcg gcgatcgcgg tgttcctgct ggctcagtcg atcctcacca
     6541 ggtacgcccg gttctgctcg cacaagctcg gcgaacaggt tctggccgaa ctgcgcgagg
     6601 acttcgtgaa gcagtccttg gcgctcccga tcggcacggt cgagaaggcc ggcaccggcg
     6661 acctgctgac ccgttcgtcg cgcgacgtcg aggcgctcgg ctggtcggtg cggttcgcga
     6721 tgccggagac gatcatcgcg ttcgtgacga cggtgttcac gattgccgcg tgcatcctcg
     6781 tcgggacgtg ggtgctcgtc cccctggtcc tcggcgtacc gctgctgttc gtcgcgaccc
     6841 ggtggtacct caagcgggcc aagcccggct acctgcggga gtcggcgtcg tacgccgcca
     6901 tcaacgccag cgtcgcggag accacggacg gcgcccggac agtggaggcc cttcgactcg
     6961 agaagcgtcg gctggagcgg atcgacgccg acatcgcgga gtcgttcgcc gcggagcgct
     7021 acaccctgtt cctgcggacg gtgtggttcc cggccgtcga ggtcggctac ctggtgccga
     7081 ccgttgtcac actgctcttc ggtggctacc tgcacagcca aggacaggtc gacatcggcg
     7141 acgtgacggc ggcgacgctg tacgtgcagg cgctgatcga cccggtcgac cggctgatct
     7201 cctggctgga cgagctgcag gtcggcgcag cctcgctggc acggctgctc ggggtcgccg
     7261 acgtgccgga cgaccggacc gccaccgggc gaactccggc gcaccaggac atcgaggccg
     7321 aggacgtgcg gttcgcctac gtcgacagtc gcgacgtcct gcacggcgtc gacctcaagg
     7381 tctcgcgtgg tgagcgggtc gcgatggtcg ggccgtccgg cgccggcaag tcgacgctgg
     7441 gccggctgct cgccggtatc catccgccac ggaccggttc ggtgacgatc ggggacgtcg
     7501 ggatgaccga gctgccgctg ggcgagctgc gcggcgaggt ggcgctcgtg acgcaggaac
     7561 accacgtgtt cgtgggaacg gtgcgcgaca acctcgcgct cgccgtcgac gacctcgaag
     7621 gagaggtcgg gcatgaccgg ttggtggcgg cactgcgggc cgtcgatgcg ctcgactggg
     7681 tgatggcgct gcccgacgga ctggagacgc acgtcggctc gggcggactg tcgctgccgc
     7741 cgtcgcaggc gcagcagctg gcgcttgccc ggttggtcct ggccgatccg cacacgctgg
     7801 tgctcgacga ggcgacgtcg ctgatcgacc cgcggtcggc gcgccacctc gagcgttccc
     7861 tcgcagccgt gctacacggc cggaccgtga ttgcgatcgc gcaccggctg ttcacggcgc
     7921 atgacgccga tcgggtggca gtggtggagg acgggcgcgt ctccgagatc gggtcgcacc
     7981 acgagctgat cgacgccgcc ggttcgtatg ccgcgttgtg gcagtcctgg cacggcgagc
     8041 acgagtccgc caccgcctga ccccacaatg gagccggtga aaaggttcct gctgcagaca
     8101 ttcgccgagc tcgatgctca gcgtccggcg ggctgccccg agctcgtgca cttcaccgag
     8161 tcgctggcca caaccgtcat cgaggagtac tcgaaccccg gtgacctggt cctcgacccg
     8221 ttcgccggtt tcggtacgac gatggtcgtc gccgaacgac tcggtcgacg cgggtacggc
     8281 gtggagctgt tgcaggaacg ggtcaaccag atcagcgcca gcctccggga ctccggcagc
     8341 atcgtcttgg gggacgcccg gaacctcgca cagctggtcc ggtcgttgcg tgggtcggtg
     8401 tcgctcgtcc tcacctcgcc gccctacatg tcgcgcaacg accaccccga gaatccgctc
     8461 gacgcctacg ccacgatgac cggcgactac ggcacgtacc tggaagagat cgccgaggtc
     8521 ttcgccgacg ccgcgacgtt gctccgtccg gacggccacg tggtggtcaa cgtcgccaac
     8581 atcgtccacg acggggtgct cacaccgctc gcctgggaca tggctcgtgc gatcagtgct
     8641 cggctcacac tcgtccagga gtgctatctc gcctgggaca gtcagccggc cgacatctcc
     8701 ggcgactact gcctggtgtt ccggcagggg tgacgtccgc accaagcccc cgctcaggtc
     8761 ccgcccgagt cagcagtgtc ccgacttctg aagaatctca tgagatcggt tgacaagtcc
     8821 gcctttcctt gttgaatgtc gcacagcgca cctttcgatc gttgctcggg actcggaggt
     8881 gctcgtcatg accgcatcgc gccatcgggg catcctgcga caccggtctg tgttctctct
     8941 cgcgactgcc atcggcacgt tcgggctgct cacgaacggc ctggtgtcga caccggcgct
     9001 ggccgccggc cgacccgacc tgcaggtcac caccgtgacg gactcacctg gcgccagagc
     9061 cccgggccag cacttcacga tcaaggtcac tgtccgcaac ggcggcagaa gccgttcgcc
     9121 gcggacatcc accgcgttct acctgtcgaa ggatgcgcga aagagcagtg gcgacgtggc
     9181 gttgatcggc cgggccgtgg tgcccgctct cgcgcccggc aagcgcgccg ctgtcgccaa
     9241 ggccgtcacg atcccgcgga cgacgccgtc cgcgttgtat cgggtgatcg cctgcgccga
     9301 tgcgagccac ggcgtacgcg agacgcacga gggcaacaac tgccgagccg cgacgccggt
     9361 cctgtccaga gtccttgtca gcccgccgct tccccatccc gacccgctcg atgtctcgac
     9421 cacggcggaa ccggacgcca ctgcatcggc ctacgtcgtg cccggctccg ccagcaccat
     9481 caccgccacc ggctcggacg gtacgacgtt cgcgctggcc atcccggcgc atgccgtggc
     9541 gcaccccatc acggtgtccc tcacccccat cgcatcgatc gtcgacaacc cgctgagcgg
     9601 agggctgggt gcagccgcag agctcggtcc cagtgggttg cagctgctcg agccggccac
     9661 cttgaggatc acgcccggtg atccctcgat cctcacaccc cctgccgggg agcagctcga
     9721 cggcttcgcc ttctccggcg ccggacgcga attccgcctg ttccctgacc tgggctcgct
     9781 gccgggcgcg tcagtgcccg acggccagat gtggttcgcg gtgcagcact tctcgggcac
     9841 ggggttcggg ctcgggacgg ccgccgacga gaccagcgtc gaaggtcaca tccccgttgc
     9901 acccgagccg acgttcgagg ctgcgatggc cacgatcgtc aacggcgtca aacgcggcga
     9961 catcccgcag caggagctcg gcccgcggct cggcgacgcg ctggtcgagg actacctctt
    10021 ccacgtcatc cccgcgttga atgccggcct ggccgcgaac gccgagcccg gggacggtga
    10081 ggccgccaag gacgtcttcc tgcactgggc gcactgggtg gcgattctcg gctacgaggg
    10141 cggcacgaac gcgaagtaca agatcacgta cggcgcgctg ctcgccgcgg gcgacgacgc
    10201 catcgcggag atgctgtcga agttcttcaa ccaggcctac cagcggtgca tcaagcacgt
    10261 gcagcccaag cggcagtacc tcttgatgtg gggcttcgcc cggtcggcct cggtgctggg
    10321 agatcccgac gacatcctgg ggaaggggtg gcaacgcagc atcaaggatt gcgaggacaa
    10381 cttccccacg acgtgggaag gcaccatcac ctacacatgg cacatcgacg cgccgacgcc
    10441 gtgcggcggg acgtgcatcg ccgactcaca gaccgggacg ggcacggtcc atgcggcgtt
    10501 cgagcccggg gggtctgcga ccggctggac gttgagcgcc gggaacgtca acgtctccta
    10561 cgtccggtcc agtacggacc tgggtaccga cggggtgtgt cagcacacct ctacgacggc
    10621 ctggacctac tccggcgcga gcacctccga ccacatgatg ctgaacaacc aaccgtcgaa
    10681 cgccttcgtc tacttcgact tcagcggctt cccgacggtc cccacacaca cggtgttcac
    10741 caccaactgt ccgcacgagg gcggctacga ggacacgagt ccgatgggac agcagttggc
    10801 cccgctcgtc tggtgcccgg agtacccgcg gcacaacccg gatgggtcga ccgacggcac
    10861 cagcggttgg atccgcggca cacggaactc ggtgggcggc cagcaggtgc tcgacttcac
    10921 ctgcacagac agctatgacc gcggtaccgg tacgagcctc aggcattggt cggtgcagac
    10981 cagcggtgtc ttcacgaaac cgtcatgacg agtgagaaaa gttcgcccga cggacacacg
    11041 cttgggtgaa caactgccgt cattcgcatc acgctcgaga tgcacggccg ctgagggcgg
    11101 acgacactgg cggtcatgtt cgtcgacgtg gccaccgacc gcgtcaggga cggcgctaca
    11161 cctgaccgca gctggacacg cacggccgtc ctcgtcgtgg tctggtgggt cgcgatcgcc
    11221 acgatcgtca tcagtgtcgg ctggctgatc acccatccgt tcctcgacgt cgtcggtccg
    11281 agggacgatg acgtcgtgcg ctggttcgcc gaccaacgaa cgccctttct cgacgacgtc
    11341 gcggagtacg gcacctgggg cggcgagacc gtggtcacac tcgtcgtggc tcccatcctg
    11401 gcgatcggct gctgggtttt gacgcgaagc gtcgtccctg cgctgttcgt cctcctcgtg
    11461 atctgcggcg tgggtggctt ctactgggcg ggcacgatgc tcgtcccgcg gcgacggcca
    11521 cccgtgcaca tcctcgaccc cggtctggtg ccgaccgaca gcttcccgtc cggccatgtc
    11581 ggaacggcgg tcgcgttgta cggcgccgtc gtcgtcctca cgtggaccta cgcacgatca
    11641 cgtcggtggt ttgtcacgcc gctgctcgtc atgcccgtgt tcgtcctgct gtcacggctc
    11701 taccagggcg cgcaccacct caccgacgtg ctcaccagtc tcatgtacgg cttcacgtgg
    11761 ctggccgtgg tggccgcact ggtgctgcgc ggccaccacc aggcggcgaa ggtcccgatg
    11821 cgttgagccg tggggctcag gccgggtgct cgcgttcggc cccggtcggc tccagcgtgt
    11881 cgtgcaggtg tcgctggacg ccgcggtcgg ggcgagccag gaagaagtac agcgcaccga
    11941 cgatggcgat gaccgcgatc acgagcagtg tgatccagtc gaggttgaag taggcccgcg
    12001 gactcgacag gccggtcgga gccaccacgt tgagcagcat cagaccgagg tagagcgcag
    12061 ccgcgatgca gaccggccat ccccacttgc cgagctggaa cctgcctgcc ggcacccagc
    12121 cccgactccg cgcgatgatc gctgcgatca ccgtgaggag gaacgacagg tagatcccgc
    12181 tgacgccgaa cgacaccagt gccgtgagcg cgttgacgtt cgcgggatag gtgatgaagc
    12241 cgat
//
LOCUS       DATBFZ010000194        13619 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_1113849, whole genome shotgun sequence.
ACCESSION   DATBFZ010000194 DATBFZ010000000
VERSION     DATBFZ010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 13619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13619
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1113849"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..996)
                     /gene="dusB"
                     /locus_tag="VH419_05910"
     CDS             complement(<1..996)
                     /gene="dusB"
                     /locus_tag="VH419_05910"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEY7043186.1"
                     /translation="MDTRTALTAADVRPQLRLGPLEVEPPVVLAPMAGITNAAYRSLC
                     REQGAGLYVCEMITSRGLVERDEVTQSMLVFDPNESVRSVQLYGVDPAYMARAATILC
                     EEYAVRHIDLNFGCPVPKVTRKGGGAALPWKRRLLAAILEATVAAAAPYGVPVTMKTR
                     MGIDAGHLTYLEAGRIAQESGCAAIALHGRTADQHYSGEADWTAIAALVDHVDIPVLG
                     NGDVWEASDALLMMEQTGCAGVVVGRGCLGRPWLFRDLAAAFGHGSAPPLPNLGEVAA
                     MMRRHAELLVAYMGEERGCKEFRKHIAWYLKGFAAGGELRQSLGLVDTLATLDALL"
     gene            complement(975..1562)
                     /locus_tag="VH419_05915"
     CDS             complement(975..1562)
                     /locus_tag="VH419_05915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043187.1"
                     /translation="MSRRLLTLGALSAAAFVASVVLSGCSGDSDDADDATSATTNADG
                     TTVAGTTLQVGQPAVVNFAAGKKHTSKVRLTVTKVEQGRVKDFERFKLNDSARRSTIY
                     YVSTRVKNLGPDALSGKTVTLYGKVSDTTVVPPVVLESTFPACDYQPLPPKFEKDDST
                     RVCMVFMAPRHGTMSEVQWRPAGSSEPISWTPVQR"
     gene            complement(1563..2948)
                     /locus_tag="VH419_05920"
     CDS             complement(1563..2948)
                     /locus_tag="VH419_05920"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012920108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HEY7043188.1"
                     /translation="MASVVDSVVSLCKRRGFVFPCGEIYGGTRSAWDYGPLGVELKEN
                     IKRQWWRSMVTSRDDVVGLDSSVILPTKTWEASGHLQQFTDPLTECESCHKRFRADHL
                     QEAVAAKKGIEDPDTIPLTDVACPNCGTRGAWTEPRQFNGMLKTYLGVIEDESGLHYL
                     RPETAQGIFLNFLNVMNSARKKPPFGIAQTGKAFRNEITPGNFIFRTREFEMMEMEYF
                     VEPGTDEEWHQTWIDLRTEWYVGLGIKRENLRHHEHPVEKLSHYAKRTVDIEYRFDLP
                     GREFDELEGIANRTDFDLTAHSEASGQDLSYFDQATDERWTPYVIEPAAGLTRSLMAF
                     MIDAYAEDEAPNTKGGVDKRVVLRLDPRLAPVKAAVLPLSRNTDLSPKARDLAAELRQ
                     RWNVEFDDAGAIGRRYRRQDEIGTPYCVTVDFDTLDDAAVTVRERDTMKQDRIALDQV
                     RDYLAQNLAGC"
     gene            complement(3078..3539)
                     /locus_tag="VH419_05925"
     CDS             complement(3078..3539)
                     /locus_tag="VH419_05925"
                     /inference="COORDINATES: protein motif:HMM:NF024183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEY7043189.1"
                     /translation="MTEQLPGYRGQDDARNAFRAGGVAVFGIGLVLTVIAFADFFIAF
                     GGSGPPTLFFLAFIGIPLMGVGGWLLLAGFGGAAAKYAAGELAPTAKNAIGSLGLAGA
                     GQTCPQCGSSVTATAKFCDECGSALSRACPSCGNANAADARFCTECGRAVT"
     gene            complement(3940..5580)
                     /locus_tag="VH419_05930"
     CDS             complement(3940..5580)
                     /locus_tag="VH419_05930"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEY7043190.1"
                     /translation="MTSEQRPAGLRRLHPLTPLFRSWRLVGLAGALGLGAFRDDLDRL
                     EWIWNALHGDAEMTVLIRAFLVVGGAALASVALGWLSWRTTGFAIVAEPGEPGTLLYH
                     RGLVNRQRSQLRLKRVQAVDVNQPLIPGLLGLAAVKLDMAAGEGASVNLSYLSLADAW
                     ELRDDILRHTSVDAEQARSGLEPRQRSRAEVIGRVQTSYLVKATLLDGAGAWLLLVVW
                     VAGVIVAGTAFGWAALGAALAGILPVTIAIFVLLRQQVTTILRDADFTVMRTPTGIRT
                     RAGLTSTTNRSIDLDRVQELRIERPMLWRWLGWARVDVDVAGAGEARGASLMPVAPDA
                     VAGALAADVAGEVLEPMRTDGIGRAPEPFVGPGARARLLDPLAWRWYGVALLERGAVT
                     RTGWRTRTVSYVPYARVQSVSARQGWLQRRLGLATVCLDMPSGTVRWVAPHRDVIEAA
                     ELVTRLSEQARRHRMPNGWSVASEPAGDAGDRSPDDGTQHDAAHQPSTRVEHRPREVR
                     QPDHEECDHDQSRDGQVQRRGESHEHEREDRARYDPRR"
     gene            complement(5577..6092)
                     /locus_tag="VH419_05935"
     CDS             complement(5577..6092)
                     /locus_tag="VH419_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020387948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEY7043191.1"
                     /translation="MKHPAAERLFVDPGLDWRPVSPKLVTERRVPVAAVGAVGLGLVI
                     VAVVVAANGSSYLGWLIPGVVILVVTAVSWFVVIPRTVSSWFYAEREQDLLVTHGRLR
                     RSLSVVPYGRMQVIEVSANPVAQHLGIAAVKLVTASASTDAMIPGLPVDEAHALRDRL
                     AAKGEALSAGL"
     gene            complement(6102..6914)
                     /locus_tag="VH419_05940"
     CDS             complement(6102..6914)
                     /locus_tag="VH419_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEY7043192.1"
                     /translation="MRIASFNVNSIRSRIDRVEAVLRRHDIDVLTIQETKARDDQWPS
                     LGLEAMGYEVATFGSNQWNGVAIASRVGLSDVRRGFDGQPHWGTPLASEARALGARCN
                     GVHVWSLYVPNGRALGDPHLAYKLEWLERLRMAAAGWLADEPDAQVALTGDWNIAPRD
                     EDVWDMRVFARSTHVSPPERAAFEAIVQSGYADLVRPYAPGPGVYTYWDYVRLRFPKN
                     KGMRIDFILGSPALQRRVTGAMIDREERKGARASDHAPVIVDLSSGSKLSTT"
     gene            6975..7877
                     /locus_tag="VH419_05945"
     CDS             6975..7877
                     /locus_tag="VH419_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012920089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEY7043193.1"
                     /translation="MWRWGRAAVAVAVLPVLAGCDALASDADDGPQVVASFYPLAYVA
                     QRVVGDHATVTNLTAPGLEPHDLELSPRQVADLSVADLVVFEKGFQPSVDEAVDQNAE
                     GTTLNVADVVPLEDTGAPTEDGSDLAGDPHVWLDPTLLEPVADKVATDMARIDPQHTA
                     AYRRNAAALDADLRQLDSAYRRGLRHCARSEFVTSHAAFGYLARRYGLTMIAIAGLSP
                     DVEPSPQHIAQIQDLITSHGITTVFSEVLGSKKYADTLAHDVGVKAAVLDPIEGLPSE
                     DSHEDYLSLMRANLTALEEANGCS"
     gene            7874..8656
                     /locus_tag="VH419_05950"
     CDS             7874..8656
                     /locus_tag="VH419_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEY7043194.1"
                     /translation="MTTDSAHAHPVVSIRAGNVALGGRPVLRGIDLTVRQGEVVAILG
                     ANGSGKSTLVRGLMGLTAWTSGDVRLLGTPLPKFHEWYRVGYVPQRVTASAGVPATVA
                     EVVGSGRLARRRLLMPMSRADRVAVRDAIAAVELSDRTHDAVAQLSGGQQQRVLIARA
                     LAGQPELFVLDEPNAGVDRHNQIGLAETLRPLVARGGTVLLVLHELGPLASLIDRAVV
                     VREGRIVYDGPPPDDAALEEFHDHHRPRQSSDHVPMRSGWDL"
     gene            8644..9642
                     /locus_tag="VH419_05955"
     CDS             8644..9642
                     /locus_tag="VH419_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEY7043195.1"
                     /translation="MGPVNLLAYDFMVRALLAATFTGVAAPAVGTYLVQRRLALMGDG
                     IGHIAVTGVGLGLLTGHSPVWTAVIVSILGAVLIELVRDRGNTSGDVALALIFYGGIA
                     GGVMITGLAGGSAQTLQNYLFGSITTVSPADVWTVVILAAIVIFIAIGFAPQLFAVCQ
                     DEEFARVMGLPVRFYTVLTSVMAAVTVTVAMRTVGLLLVSALMVVPVATAQQVARGFK
                     VTILVAMLLGAAASIGGVVTSFYVDVAPGATIVLLALAVFVAAWPVGVLLRHRRRLAE
                     PFPVGDEAMPHDVVPETHAHQHGADCGHQAVEHGDHVDYVHGGHRHAMHGDHYDEH"
     gene            9658..10086
                     /locus_tag="VH419_05960"
     CDS             9658..10086
                     /locus_tag="VH419_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020386004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HEY7043196.1"
                     /translation="MVTQRPGDGAVTVGARPTRQRKAVVAALGDSDVFRSAQDIHAAL
                     RERGADVGLTTVYRTLQALADSGDVDVLRTPDGESVYRRCSTGHHHHLVCRGCGRTVE
                     IEGPAVESWATKTAHAHGFAEVEHTLEIFGICESCAERAD"
     gene            complement(10190..10975)
                     /locus_tag="VH419_05965"
     CDS             complement(10190..10975)
                     /locus_tag="VH419_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002548545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEY7043197.1"
                     /translation="MTNRATEGGKAGNRRPSYDRPPDVPPELVPRHIAVIMDGNGRWA
                     RQRGLPRTAGHERGEAALLDVVKGGVEAGVEAISAYAFSTENWSRSPDEVRFLMGFNR
                     DVIRRRRDEMHAMGVRVRWAGRRPRLWSSVIKELEIAEELTRDNTVLTLTMCVNYGGR
                     AEIADAAAELARQAVAGTLKPGKIDEKRVARALYVPELPDVDMVLRTSGEQRFSNFML
                     WQAAYAEMVFMDVLWPDVTRRTLYEAIDIYARRDRRYGTASSD"
     gene            11096..12757
                     /locus_tag="VH419_05970"
     CDS             11096..12757
                     /locus_tag="VH419_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HEY7043198.1"
                     /translation="MSDRPASAPATSATDATPATPEMPQLSHRQVLTVLTGLMLGMLV
                     AALSQTIVATALPTIVGELGGQDQLSWVVSATLLASTASTPIWGKLSDLYGRKIMYQL
                     AIWTFIGGSFLAGFSQNMAMLIGSRAIQGLGIGGLMALGQAIIGDIVSPRERGRYQGY
                     MGSAFGLATVLGPLIGGVIVEHLSWRWTFFVGIPVGLAALIVTERVLQLPFPRREHKV
                     DWLGAFFIVAGVSSILLVLSLGGDAFAWSSAWTVAISLLAVCFLTLAVWQERRASEPI
                     MPPRLFRNHTFVICSIAGLAVGIAMFGAIVFLPQYLQIVRGRSPMMSGLLTLPLMLGM
                     IVAMTLSGRTITRTGRYKIFPVVGMLVLACGLVLMSRLHADTSLLVADVYMGITGIGI
                     GMSMQVLVLATQNAVDRADMGVATSGATFFRSMGGAIGVAMFGALLRHENASTIPDRI
                     REFKVPIAQLTPGNLSIGTPADVAKLPGALHDAVVYGFADSMQVVFFSAAPFAILGFV
                     VVLFLREQRLSTRHAEPADVADASLDPLDSGKDPDVIPATATKSR"
     gene            complement(12931..>13619)
                     /gene="recO"
                     /locus_tag="VH419_05975"
     CDS             complement(12931..>13619)
                     /gene="recO"
                     /locus_tag="VH419_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEY7043199.1"
                     /translation="KLGEADRIVTFLTRRTGRVRAVGKGVRRTKSRFGSRLEPFMQVD
                     VQFARGRSLDVVTQAETLAPFGEAIAGDYARYTAGTAMLETAERLVAEEMEPALQQYL
                     LLVGGLRVLASGDRDPGLVLDSFLLRSLATAGYAPSFEECARCGVEGPHRSFSAGAGG
                     MLCSSCRMPGVATPAPEAVTLLGALLAGDWVTAESVDPRFRKPASGIVAAYLQWHLER
                     GLRSLAHVER"
BASE COUNT         2129 a         4771 c         4503 g         2216 t
ORIGIN      
        1 cagcagtgcg tcgagggtgg ccagcgtgtc gaccagaccg agcgactgcc ggagctcgcc
       61 gccggcggcg aagcccttga ggtaccacgc gatgtgcttg cggaactcct tgcaccctcg
      121 ctcctcgccc atgtacgcga cgagcagctc ggcatggcgg cgcatcatcg cggcgacctc
      181 gcccaggttg ggcagcggcg gcgccgaacc gtggccgaac gccgcggcca ggtcacggaa
      241 caaccacggc cgacccaggc agccacggcc gaccacgact cccgcacagc cggtctgctc
      301 catcatcagc agggcgtcgg aggcttccca gacgtcgccg ttcccgagca ccgggatgtc
      361 gacgtggtcg acgagcgccg cgatcgccgt ccagtcggcc tcgccggagt agtgctggtc
      421 ggcggttcgt ccgtgcaacg cgatggcagc gcagccggac tcctgtgcga tgcggccggc
      481 ctcgagatac gtcaggtgcc cggcgtcgat gcccatccgg gtcttcatcg tgacgggtac
      541 gccgtacggc gccgctgccg cgaccgtggc ttcgaggatc gccgccagca gccggcgctt
      601 ccacggcagg gcggcaccgc cccccttgcg agtgaccttg gggacgggac agccgaagtt
      661 gaggtcgatg tggcggacgg cgtactcctc gcacaggatc gtcgctgcgc gcgccatgta
      721 cgccgggtcg acaccgtaga gctgaaccga gcgcacggac tcgttcgggt cgaacacgag
      781 catcgactgc gtcacctcat cacgctcgac gaggccacgg ctggtgatca tctcgcagac
      841 gtataggccg gcgccctgtt cacgacacag cgagcggtag gcggcgttcg tgatgcccgc
      901 catcggcgcg agcaccactg gcggctcgac ctcgagcggc ccgagtcgga gctgcggccg
      961 gacgtcggcg gccgtcaacg ctgtacgggt gtccacgaga tcggctcgga gctgccggcc
     1021 ggacgccact gcacctcaga catcgtgccg tggcgcggtg ccatgaacac catgcagact
     1081 ctcgtcgagt cgtccttctc gaacttggga ggaagcggct ggtagtcgca ggccgggaat
     1141 gtggactcga gcacgacggg agggaccacg gtggtgtccg agaccttgcc gtagagggtc
     1201 acggtcttgc cggacagtgc gtccgggccc aggttcttca cgcgcgtcga cacgtagtag
     1261 atcgtggacc ggcgcgccga gtcgttgagc ttgaaccgct cgaagtcctt gaccctgccc
     1321 tgctcgacct tggtgacggt cagccggacc ttcgacgtgt gcttcttgcc ggcggcgaag
     1381 ttcaccaccg caggctggcc gacctgcaac gtcgtaccgg cgaccgtcgt gccgtcggcg
     1441 ttcgtcgtgg cgctggtcgc atcgtcggcg tcatcggagt cgccactgca ccccgacaac
     1501 accaccgacg ccacgaaggc ggccgcggac aacgccccca aggtcagcag gcggcggctc
     1561 attcagcagc cggccaggtt ctgcgcgagg tagtcgcgta cctggtcgag cgcgatccgg
     1621 tcctgcttca tcgtgtcgcg ctcgcgcacg gtcaccgcgg cgtcatcgag cgtgtcgaag
     1681 tcgaccgtca cgcagtacgg cgtcccgatc tcgtcctgtc gccggtaccg acggccgatg
     1741 gcacctgcgt cgtcgaactc gacgttccaa cgctgccgca gctcggcggc gaggtcgcgt
     1801 gccttcggcg agaggtcggt gttccgcgac agcggcagga cggcggcctt caccggggcc
     1861 agccgggggt cgaggcgcag cacgacgcgc ttgtcgacgc cgcccttggt gttcggcgcc
     1921 tcgtcctcgg cgtacgcgtc gatcatgaac gccatcagcg acctggtcag accggccgcg
     1981 ggttcgatga cgtacggcgt ccagcgttcg tcggtcgcct ggtcgaagta cgacaggtcc
     2041 tgaccggaag cctcgctgtg ggcggtgagg tcgaagtccg tgcggttcgc gatgccctcg
     2101 agctcgtcga actcacggcc cggcaggtcg aagcggtact cgatgtcgac cgtgcgcttg
     2161 gcgtagtgcg acagcttctc gaccgggtgc tcgtggtgac gcaggttctc ccgcttgatg
     2221 cccagaccga cgtaccactc ggtgcgcagg tcgatccagg tctgatgcca ctcttcgtcg
     2281 gtgccgggct cgacgaagta ctccatctcc atcatctcga actcgcgcgt gcggaagatg
     2341 aagttgcccg gggtgatctc gttgcggaag gccttgccgg tctgcgcgat gccgaacggc
     2401 ggcttcttgc gcgcggagtt catcacgttg aggaagttca ggaagatgcc ttgcgcggtc
     2461 tccggtcgca ggtagtgcag gccgctctcg tcctcgatca cgccgaggta ggtcttgagc
     2521 atgccgttga actggcgtgg ctcggtccac gcgccgcgcg tcccgcagtt cgggcaggcc
     2581 acgtcggtca gcgggatcgt gtccgggtct tcgatgccct tcttggccgc caccgcctcc
     2641 tgcagatggt cggcgcggaa ccgcttgtga cagctttcgc actcggtcag cggatcggtg
     2701 aactgctgaa ggtgtccgga tgcttcccac gtcttcgtcg gaaggatcac cgacgagtcg
     2761 agccccacga cgtcgtcacg tgacgtgacc atggaccgcc accactggcg cttgatgttc
     2821 tccttgagct cgacgccgag cggcccgtag tcccaggcgg agcgggtgcc tccgtagatc
     2881 tcgccacagg ggaagacgaa tccccggcgc ttgcagaggc tgacgacgct gtcgacgact
     2941 gatgccattg gagctctcca tccggctggc ggtcggtggt caactccccg atgtggagag
     3001 cacctccagc ctaacgaccg gtgtcgacaa gattgcgacc cgggtccgga gcgtggcgct
     3061 ggagcgttgt gtcggagtca ggtgaccgcc cggccgcact cggtgcagaa cctggcatcg
     3121 gccgcgttgg cgtttccaca ggaggggcag gccctggaca gcgcagaccc gcactcgtcg
     3181 cagaacttgg cggtggcggt gaccgacgac ccgcactgtg ggcaggtctg accggcgccg
     3241 gcgaggccca ggcttccgat cgcgttcttc gccgtcggcg caagctctcc ggcggcgtac
     3301 ttggccgccg ctccgccgaa acctgccagc agcagccaac cgcccacccc catcagcggg
     3361 atcccgatga aggccaggaa gaacagcgtc ggcgggccgg agccgccgaa cgcgatgaag
     3421 aagtcggcga aggcgatgac ggtgaggacg agaccgatcc cgaagaccgc gacaccacct
     3481 gcccggaagg cgttgcgggc atcgtcctga ccgcggtagc cgggaagttg ctcagtcatg
     3541 ggcgggagtg tacgacgtcg acgggccggc atgtgatgct tcgggatctc ctcaggtgtc
     3601 tgcgcgatgt tgacatctga agagacccca cacatcgcag acccacaccg tccaccgcag
     3661 atcctcgatg ttcacaggcc aagagggctg taaacatcgc aaacctcgaa gcgtttgcat
     3721 gcgcgatccc tggctgactg cccatcgtcg aagcccgccc gcctcgacaa gctcgaacag
     3781 aggactgggc tcgagcccta ggatgatgcg gtggccacac ggaatccgaa gcgctccggg
     3841 aacccggctg tccgggcggg cgcaggcgac acaccgccgt cggccacccg caggcggttg
     3901 agcgaacgaa gccgcggcgt cctcctccgg ttgtcgcagc taccgccgtg ggtcgtacct
     3961 ggcgcggtcc tcgcgctcat gctcgtggga ctcaccgcga cgctgtacct ggccatcccg
     4021 gctctggtcg tgatcgcact cttcgtgatc tggctggcgt acctctcgtg gccggtgctc
     4081 gacacgggtg gacggctggt gcgcggcatc atgctgggtg ccgtcatcgg ggctgcgatc
     4141 gcccgcatca ccggctggct ctgacgcgac agaccaccca ttcggcatac gatgccgtcg
     4201 cgcctgttcg ctcagcctcg tcaccagctc ggcagcctcg atgacatcgc gatgtggagc
     4261 gacccaccgg acagtcccgc tcggcatgtc gagacacacg gtcgccagac cgagccgccg
     4321 ctgcagccaa ccctgacgcg ccgacacact ctggacccgc gcgtacggca cgtagctgac
     4381 ggttcgggtg cgccaccccg tcctggtgac ggcgcctcgc tccagcaacg ccacgccgta
     4441 ccagcgccac gccaacgggt ccagcagtct cgcccgcgcg ccagggccga cgaacggctc
     4501 gggcgcacga ccgatgccat ccgtccgcat cggctccagc acctcaccgg cgacgtcggc
     4561 cgccagcgcg ccggccacgg catccggcgc caccggcatc aacgacgcgc ctctcgcctc
     4621 gcccgcaccg gcaacgtcga cgtcgacccg ggcccacccg agccagcgcc acagcatcgg
     4681 gcgctcgatc cgcagctcct gcacccgatc gaggtcgatg ctccggttgg tggtcgaggt
     4741 gaggccggcg cgggtcctga tgccggtagg cgtccgcatc acagtgaaat ccgcgtcgcg
     4801 caggatcgtc gtgacctgtt ggcgcaggag cacgaaaatc gcgatcgtca ccggcaggat
     4861 gccggccagc gctgcgccga gcgccgccca gccgaacgcg gtgcccgcca cgatgactcc
     4921 ggccacccag acgacgagca acagccaggc cccggcgccg tcgagcaacg tcgccttcac
     4981 gaggtacgac gtctggacgc gaccgatcac ctcggcccgg ctgcgctgcc gtggttcgag
     5041 tccggatctc gcctgctccg catcgaccga ggtgtgccga aggatgtcgt cgcgcagctc
     5101 ccacgcatcg gcgaggctga ggtaggagag gttgaccgaa gcgccctcgc cggccgccat
     5161 gtcgagcttg accgcggcta atccaagcag gccgggaatg agcggctggt tgacgtcgac
     5221 cgcctggaca cgcttcaacc gcagttggct gcgctgccgg ttgaccaggc cgcggtggta
     5281 cagcagcgtg cccggttcac cgggttcggc cacgatcgcg aaccccgtcg tacgccacga
     5341 cagccaaccc agcgcgaccg aggccagtgc cgcgccacca acgacgagga aagcccggat
     5401 cagcacggtc atctcggcgt caccgtgcag cgcgttccag atccactcga gccggtccag
     5461 gtcgtcgcgg aacgccccga gacccaacgc gccggcaagg ccgacaaggc gccaggaccg
     5521 gaacagcggc gtgagcgggt gcagccgccg cagaccagcc gggcgctgct cggacgtcac
     5581 agccccgccg acagtgcttc gcccttggcg gcgagccggt cgcggagcgc atgcgcctcg
     5641 tcgacgggca agcccgggat catcgcgtcc gtggacgccg acgccgtcac gagcttgacc
     5701 gcggcgatgc cgaggtgttg tgccaccggg ttggcggaaa cctcgatcac ctgcatgcgt
     5761 ccatacggca cgacggacag tgaacgccga agccgtccat gcgtgacgag caggtcctgc
     5821 tcgcgttcgg cgtagaacca ggacgacacg gtgcgcggaa tcaccacgaa ccagctcacc
     5881 gccgtcacga ccaagatgac gacgccggga atcagccagc cgaggtacga cgacccgttc
     5941 gccgcgacca cgacggcgac gatcacgagt ccgagaccga cggccccgac agcagccacc
     6001 gggacgcggc gttcggtcac gagcttcggc gacaccggcc gccagtcgag tcctggatcg
     6061 acgaagaggc gctccgcagc gggatgcttc acgaattcag actaggtcgt gctgagcttc
     6121 gagccgctgc tgaggtcgac gatgaccggg gcgtgatcac tggcccgagc gccctttcgt
     6181 tcctctcgat cgatcatcgc gccggtcacc cgtcgttgca gggccggtga tccgaggatg
     6241 aagtcgatcc gcatcccctt gttcttgggg aaccgcaggc gcacgtagtc ccagtacgtg
     6301 tagacacccg gccccggcgc gtacggccgc accagatcgg cgtacccgct ctgcacgatg
     6361 gcctcgaacg ccgctcgctc agggggcgac acatgcgtgc tgcgagcgaa cacccgcatg
     6421 tcccacacgt cctcgtcgcg cggcgcgatg ttccagtccc cggtcaacgc gacctgcgcg
     6481 tcgggctcgt ccgccaacca gccggcggcc gccatcctga gacgttccag ccattcgagc
     6541 ttgtaggcca ggtggggatc gccgagtgcc cgaccgttcg ggacgtacaa cgaccagacg
     6601 tggacaccat tgcagcgggc cccgagcgct cgtgcctccg aggccaacgg cgtcccccag
     6661 tgtggctgcc cgtcgaaccc acggcgtacg tcggacaacc cgacccggga ggcgatggcg
     6721 acgccgttcc actggttgga gccgaaggtc gcgacctcgt accccatcgc ctccagtccc
     6781 agcgagggcc actggtcgtc acgggccttc gtctcctgga tggtgaggac gtcgatgtcg
     6841 tggcggcgca gcaccgcttc cacccgatcg atccgggacc ggatcgagtt cacgttgaac
     6901 gaggcgatcc gcacgacgcc accgtagcgg gccacagttg acaatggttt tcagttcagt
     6961 tgacaatggt tttcatgtgg cgttggggca gagcggctgt ggcggtcgcc gtgctgcccg
     7021 tactcgccgg atgcgacgcg ctggcgagcg acgccgacga cggcccgcag gtggtggcct
     7081 ccttctaccc gctggcgtac gtcgcccaac gggtcgtcgg tgaccacgcc acggtcacca
     7141 acctcaccgc acccggcctc gagccgcacg atctggaact gtccccgcgc caggtcgccg
     7201 acctgagcgt ggcggatctg gtcgtcttcg agaagggctt ccagccgtcc gtcgacgaag
     7261 ccgtcgacca gaacgccgaa ggcacgacgc tgaacgtcgc cgacgtcgta ccactggagg
     7321 acaccggtgc gcccaccgag gacggttccg acctcgccgg cgacccgcac gtctggctgg
     7381 acccgacgct tctggagccg gttgccgaca aggtcgccac cgacatggcc cggatcgatc
     7441 cccagcacac cgcggcgtac cgtcgcaacg ccgccgcgct ggacgcggat ctgcggcaac
     7501 tcgactcggc gtaccggcgt ggtttgcgcc actgcgcgcg cagcgagttc gtcaccagcc
     7561 acgcggcgtt cggctatctc gctcgacgct acggcctcac gatgatcgcc atcgccggtc
     7621 tgtcaccgga cgtggagccc agtccccagc acatcgcaca gatccaggac ctcatcacgt
     7681 cgcacggcat cacgacggtg ttctccgagg tgctgggcag caagaagtac gccgacacgc
     7741 tagcgcacga cgtcggagtc aaggccgcgg tcctggaccc catcgaaggt cttccgagcg
     7801 aggactcgca cgaggactac ctctcactca tgcgcgccaa cctcaccgca ctggaggagg
     7861 cgaacggctg ctcatgacca ccgactccgc ccacgcccac ccggttgtgt cgatccgcgc
     7921 gggcaacgtc gctctcgggg gacgtcccgt gctgcgggga atcgacctca ccgtccgcca
     7981 gggagaggtg gtcgcgatcc tcggcgcgaa cggctccggg aagtccaccc ttgttcgcgg
     8041 tctgatgggc ctcactgcgt ggacgtcagg tgacgtgcgc ctgctcggta cgccgctgcc
     8101 gaagttccac gagtggtacc gggtcggcta cgttccccag cgcgtcacgg cgagcgccgg
     8161 cgtaccggcc accgtggcgg aggtcgtcgg ttccggccgg ctggcccggc gtcggctgct
     8221 gatgccgatg agccgggcgg accgtgtggc ggtccgcgac gcgatcgctg cggtcgagct
     8281 ttccgaccgc acccacgacg cggtcgctca gctgtcgggt gggcagcaac agcgcgtcct
     8341 catcgcgcgc gcactcgcgg gacaacccga gctgttcgtg ctcgacgaac cgaacgccgg
     8401 ggtggaccga cacaaccaga tcggcctggc cgagacgctg cgaccgctgg tcgcgcgcgg
     8461 gggaaccgtg ctgctcgtgc tgcacgagct cggcccgttg gcctcgctca tcgatcgcgc
     8521 cgtcgtcgta cgagaggggc gcatcgtgta cgacggtccg ccacctgacg atgcggcact
     8581 cgaggagttc cacgaccatc accggccgcg ccagtcgtcc gaccacgtgc cgatgcggag
     8641 cgggtgggac ctgtgaacct gctcgcctac gacttcatgg tccgggccct gctcgcggcc
     8701 acgttcaccg gggtggccgc cccggcggtg ggcacgtacc tggtgcagcg ccggctggcc
     8761 ctgatgggtg acggcatcgg ccacatcgcg gtcaccggcg tcgggctcgg tctgctgacc
     8821 gggcactccc ccgtctggac cgctgtgatc gtgtcgatcc tcggtgccgt gctcatcgag
     8881 ctggtgcgcg atcgcggcaa caccagcggt gacgtggcac tcgcactcat cttctacggc
     8941 ggtatcgcgg gtggggtgat gatcaccggc ctggcgggcg ggagcgcaca gacgctgcag
     9001 aactacctgt tcgggtcgat caccaccgtg tcgcccgcgg acgtgtggac cgtggtgatc
     9061 ctggcggcga tcgtgatctt catcgcgatc ggctttgcac cgcagctgtt cgcggtctgc
     9121 caggacgagg agttcgcgcg ggtcatgggc ctgccggtcc gcttctacac cgtgctcacg
     9181 tcggtgatgg cggccgtcac ggtgactgtg gcgatgcgta cggtcggcct gctgttggtc
     9241 agcgcgctca tggtcgtccc ggtggcgacc gcacagcagg tggcacgagg gttcaaggtg
     9301 acgatcctgg tggcgatgct gctgggtgcc gctgcctcca tcggtggtgt ggtcacgagc
     9361 ttctacgtcg atgtggcacc tggtgcgacc atcgtcttgc ttgccctcgc cgtgttcgtg
     9421 gcggcctggc cggtcggtgt gctgctgcgc catcgacgcc ggttggccga accgttcccc
     9481 gtcggcgacg aggccatgcc gcacgacgtc gtacccgaga cgcacgccca tcagcacggc
     9541 gccgactgcg gccatcaagc cgtcgaacac ggcgaccacg tcgactacgt ccacggcggg
     9601 catcgccacg cgatgcacgg cgaccactac gacgagcact gaggacggca ccaagcgatg
     9661 gtgacgcaac gccccggcga cggcgcggtg acggtcggcg cccgacccac ccggcaacgc
     9721 aaggccgtgg tggccgcgct cggtgacagc gatgtcttcc gcagtgcgca agacattcac
     9781 gcggccttgc gggagcgcgg cgccgacgtc ggactcacga ccgtctatcg cacgctgcag
     9841 gccttggccg acagcggtga cgtcgacgtg ctgcgtacgc cggatggcga gtcggtctat
     9901 cgcaggtgca gcaccggcca tcaccaccac ctggtgtgcc gaggctgcgg tcgaacggtc
     9961 gagatcgagg gtcccgccgt cgagtcgtgg gcgaccaaga cggcgcacgc ccacgggttc
    10021 gccgaagtgg agcacacgct ggagatcttc ggtatctgcg agtcctgcgc cgaacgggcc
    10081 gactgacccc tttccgtccg cttgggtctc tgcgaattgc gggaaagtgc aggtcccgag
    10141 cgcggttcga gcatgacgtt tcccgcaatt cgcagaagcc cggcagcgat tagtcggagg
    10201 aggcggtgcc gtagcgacgg tcgcggcgcg cgtagatgtc gatcgcctcg tacagcgtgc
    10261 gtctcgtgac atcgggccag agcacgtcca tgaacaccat ctccgcgtac gccgcctgcc
    10321 agagcatgaa gttgctgaac cgctgctcgc cggacgtcct cagcaccatg tcgacgtcgg
    10381 ggagctccgg gacgtagagc gctcgcgcga ctcgtttctc gtcgatcttt cccggcttca
    10441 acgtgccggc caccgcctgc cgcgccagtt ccgcagctgc gtccgcgatc tctgcgcggc
    10501 ctccgtagtt gacgcacatc gtcagggtca agacggtgtt gtcgcgggtc agctcctccg
    10561 cgatctcgag ctccttgatg actgagctcc acagccgcgg ccgccgtccc gcccaccgga
    10621 cgcgcacgcc catcgcatgc atctcgtcac ggcgacggcg gatcacgtcg cggttgaacc
    10681 ccatgaggaa cctgacctcg tccggtgatc gcgaccagtt ctcggtcgag aacgcatacg
    10741 ccgagatcgc ctcgactccg gcctcgaccc cgcccttgac cacgtccagc agcgcggcct
    10801 cgccccgctc gtgtccggcc gtacgcggca ggccacgttg ccgcgcccac cggccgttgc
    10861 cgtccatgat caccgcgatg tgtcgcggaa cgagctccgg cggcacgtcg ggtggccggt
    10921 cgtacgacgg gcgccggttg cccgctttcc cgccttcggt cgcgcggttg gtcacgcgca
    10981 tcactgtggc atctcggctg atttgccgac cagcccgttg cggacatata gtgatcctat
    11041 ctagttgcat ggcacattca aatgttcccc cactcgtcga ggagactccc gcttcgtgag
    11101 cgaccgcccc gcttccgctc ccgccacctc ggccaccgac gcgacaccgg cgacaccgga
    11161 gatgccgcag ctctcccacc gacaagtgct cacggtgctc accgggctga tgttggggat
    11221 gctggtcgcc gcgctgtccc agaccatcgt cgccaccgcc ctgcccacca tcgtcggtga
    11281 gctcggcggt caggaccagc tgtcctgggt cgtctcggcg acgctgctcg cgagcactgc
    11341 atcgacgccg atctggggca agctgtcaga cctctacggc cgcaagatca tgtaccagct
    11401 cgcgatctgg acgttcatcg gcggctcgtt cctggcgggc ttctcgcaga acatggcgat
    11461 gctcatcggc tcacgtgcga tccagggcct cggcatcggt ggcctgatgg cgctcggcca
    11521 ggcgatcatc ggcgacatcg tctcgccgcg agagcgaggc cggtaccagg gctacatggg
    11581 gtcggcgttc ggcctggcca ccgttctcgg tccgctcatc ggaggcgtga tcgtcgagca
    11641 cctgtcctgg cgctggacgt tcttcgtcgg tatcccggtc gggctggcgg ctctcatcgt
    11701 caccgagcgg gtcctgcagc ttccattccc gcgtcgcgag cacaaggtcg actggctcgg
    11761 tgcgttcttc atcgtcgccg gggtgagcag catcctgctc gtgctgtcgc tcggtgggga
    11821 tgccttcgcc tggtcctccg cctggaccgt cgcgatctcc ctgctcgccg tctgcttcct
    11881 cacgctcgcc gtctggcagg agcgcagggc gtcggagccg atcatgccgc cacgactgtt
    11941 ccgcaaccac acgttcgtga tctgcagcat cgcgggtctg gcggtcggca tcgcgatgtt
    12001 cggggcgatc gtcttccttc cgcagtactt gcagatcgtt cgcggtcgat cgccgatgat
    12061 gtccggcctg ctgacgctgc cgctgatgct cggcatgatc gtcgcgatga ccctcagcgg
    12121 ccgcaccatc accaggaccg gtcgctacaa gatcttcccg gtcgtcggca tgctcgtcct
    12181 ggcctgcggt ctggtgctga tgtcaaggct gcacgccgat acgtcgctgc tggtcgccga
    12241 cgtctacatg ggcatcaccg gtatcgggat cggtatgtcg atgcaggtgc tcgtactcgc
    12301 gacgcagaac gccgtcgacc gcgccgacat gggtgtggcc acgtcgggtg cgaccttctt
    12361 ccgctcgatg ggcggcgcga tcggtgtggc gatgttcggc gcgctgctgc ggcacgagaa
    12421 tgccagcacc atccccgacc gcatcaggga gttcaaggtc ccgatcgccc agctgactcc
    12481 tggcaacctg tcgatcggga cgccggcaga cgtggcgaag ctgccggggg cgcttcatga
    12541 cgccgtcgtc tacggcttcg ccgactcgat gcaggtcgtg ttcttctcgg ccgcgccgtt
    12601 cgcgatcctg ggattcgtcg tcgtgctgtt cttgcgtgag caacggctga gcacccgtca
    12661 cgccgaaccc gcggacgtcg ccgacgcctc attggacccg ctcgactccg gcaaggaccc
    12721 ggacgtcata cctgcaacgg caaccaagtc ccgctgatcg ccggtcttcc gttcgttgag
    12781 cttgtcgaaa ccaaggaacc ccaggaggtc tcgacaagct cgacgaccga gggcggggac
    12841 tcgacaagct cgaccaccga gggcggggac tcgacacgct cgaccaccga agcacgccga
    12901 ccagctcgac caccgaagca gagtgtgagg tcagcgttct acgtgggcga gcgaacggag
    12961 gccgcgctcg aggtgccact gcaggtacgc cgccacgatc ccgctggcgg gcttgcgaaa
    13021 cctcggatcg accgactcgg cggtcaccca gtcgccggcg agcagcgcac cgagcagggt
    13081 gacggcctcc ggcgcgggtg tcgccacgcc gggcatccgg caactcgagc acagcatccc
    13141 accggcgcct gcgctgaagc tgcgatgtgg tccttcgacg ccgcaccgtg cgcattcctc
    13201 gaacgagggc gcgtaaccag cggtcgccaa cgaccgcagg aggaacgagt cgagtaccag
    13261 acctgggtcg cgatctccag aggcgagcac acgaagcccg ccgacgagca gcagatactg
    13321 ctgcagcgcc ggctccatct cctccgcgac gagtcgttcg gccgtctcga gcatcgctgt
    13381 cccggcggtg tagcgggcgt agtcccccgc gatcgcctcg ccgaacggcg ccagcgtctc
    13441 ggcttgcgtc accacgtcga gactgcggcc acgggcgaac tgcacgtcca cctgcatgaa
    13501 cggctcgagc cgcgagccga accgtgactt cgtccggcgt acacccttgc cgaccgcacg
    13561 cacccgcccg gtccgccgcg tcaggaaggt gacgatccgg tccgcctcgc ccagcttct
//
LOCUS       DATBFZ010000195         1527 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_4988997, whole genome shotgun sequence.
ACCESSION   DATBFZ010000195 DATBFZ010000000
VERSION     DATBFZ010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4988997"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            86..1180
                     /gene="ilvC"
                     /locus_tag="VH419_05980"
     CDS             86..1180
                     /gene="ilvC"
                     /locus_tag="VH419_05980"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002524151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEY7043200.1"
                     /translation="MRPTDPRSKAARKVGRIEKENPMAELFYDDDADLSVIQGRNVAV
                     IGYGSQGHAHALSLRDSGVDVRVGLPEASKSRPKAEAEGLRVVTPYEACEESDLIVVL
                     APDPAQRSLYAEAIEPNLVDGDALFFGHGFNIRFGYITPPTGVDVAMVAPKGPGHLVR
                     REFEQGRGVPVLVAVEQDASGKAWQLTLSYAKAIGGTRAGAIRTTFTEETETDLFGEQ
                     AVLCGGASALIQAGFETLVEAGYQPEIAYFECLHELKLIVDLMYEGGIAKQRWSVSDT
                     AEYGDYTSGPFIIDDSVRERMKQVLAHVQDGSWAAGFIADQDAGAPKFKEYRAKSEAH
                     PIEQTGRELRKLMAWVKSADDDYTEGTAAR"
     gene            1329..>1527
                     /locus_tag="VH419_05985"
     CDS             1329..>1527
                     /locus_tag="VH419_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043201.1"
                     /translation="MARSPRQRVRASLVYTTAAVALLVPLACTSDGDDAARPDASEGP
                     EAATSRIDPHRQPPAEKVEGAR"
BASE COUNT          281 a          502 c          523 g          221 t
ORIGIN      
        1 gctcgcgctc gatcaccgac cgaagcctga gaccggcacc gcccatcagc acccgcaacc
       61 ccgcctgact acgagcccga ccacgattcg tccgactgac ccacgttcga aagcggcgcg
      121 gaaggtcgga cgaatcgaga aggagaaccc gatggctgaa ctgttctacg acgacgacgc
      181 cgacctgagc gtcatccagg gtcgcaacgt tgcggtgatc ggctacggca gtcagggtca
      241 cgcccacgcc ttgtcgctac gtgactcagg tgtcgacgtc cgggtcggtc tgcctgaggc
      301 gtccaagagc cgtcccaagg cggaggcgga agggctgcgc gtcgtcacgc cgtacgaggc
      361 gtgcgaggag tcggacctga tcgtcgtcct cgcacccgat ccggcgcagc ggtcgctgta
      421 cgccgaggcg atcgagccga acctggtcga cggagacgcg ttgttcttcg gacacggatt
      481 caacatccgg ttcggctaca tcaccccgcc gaccggagtc gacgtggcca tggtcgcgcc
      541 gaaggggccg ggccatctcg tacgccgcga gttcgagcag ggcaggggag tgccggtgct
      601 cgtggccgtc gagcaggatg cctcgggaaa ggcgtggcag ctcacgctgt cctacgcgaa
      661 ggcgatcggc gggaccaggg ccggcgcgat caggacgacg ttcaccgagg agaccgagac
      721 cgacctgttc ggtgagcagg ccgtcttgtg cggcggcgcc tcagcgctca tccaggccgg
      781 cttcgagacg ctcgtcgagg ccggttacca accagagatc gcgtacttcg aatgtctgca
      841 cgagctcaag ctgatcgtgg acctgatgta cgaaggcggg atcgccaagc agcgctggtc
      901 cgtgtcggac acggcggagt acggcgacta cacctccggg ccgttcatca tcgacgactc
      961 ggtgcgggag cggatgaagc aggtgctggc gcatgtccaa gacggcagct gggccgccgg
     1021 cttcatcgcc gaccaggacg cgggcgcgcc gaagttcaag gagtaccgcg cgaagagcga
     1081 ggcgcatccg atcgagcaga ccggccgtga gctgcgcaag ctgatggcct gggtcaagtc
     1141 ggccgacgac gactacaccg aaggcaccgc cgcccgctga catgctcgtc cgtaccgagg
     1201 tgtcgatccg gtcccttgca ccggcgtgtc cagggcgaaa cctggcacct cggcgagaca
     1261 tgctcgggca caaggagtag cctcgcgcgt gtatcagcgc ggagacctgg agtgccaggc
     1321 ggactgacat ggcccgttca ccgcgtcagc gagtccgggc aagtctggtc tacacgacgg
     1381 ccgcggtcgc cctcctcgta cccctcgcct gcacgtccga tggtgacgac gcggcgcgac
     1441 ccgatgcgtc cgaaggtcct gaggccgcga ccagcaggat cgatccacac cgacaaccgc
     1501 cggccgagaa ggtcgaagga gcgcgtc
//
LOCUS       DATBFZ010000196         4606 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_2751392, whole genome shotgun sequence.
ACCESSION   DATBFZ010000196 DATBFZ010000000
VERSION     DATBFZ010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 4606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4606
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2751392"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1016..1669
                     /locus_tag="VH419_05990"
     CDS             1016..1669
                     /locus_tag="VH419_05990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043202.1"
                     /translation="MPTRLPSTRERRPALAALAVLLIVGGAVLAGWFALRQSQTESYL
                     VIDETVHTGEKIQPSDLREIELPADSGDLIPASQADDVIDSYAQVDLLQGTVVAAGMY
                     GDAPELAADEDLVGLDLDPSQFPKSIHPGDSVVVVLSAAEGSTPILLSDGTVRAIKGA
                     ETGGGSTVDVVISNQCSDQMATGSSHGDVSLVEVAPNARAITCASPDQVAKLPKGNP"
     gene            1670..2608
                     /locus_tag="VH419_05995"
     CDS             1670..2608
                     /locus_tag="VH419_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043203.1"
                     /translation="MALVTVAAAKGSPGVTTTTLVLGALWPRQVLVAECDVAGADIPL
                     RMSAADSGVLDADRGLLSLAAAGRKGLNSDLVLTHSQQVLGGMDVLVGPRVPEQAAGL
                     TNLWPMLGPALDGIPGYDVIADCGRIGADTPQNVLLRASRLLVLVCTVEPSSVVHLRE
                     RLTTLAPMLDPSSPVGTPIAVVVVADPKARDAVAQARQSLERTEIPLQGVWHLAYDPK
                     GAGFFKGHVVGRADKTMLVKTARPAVEQVAALVEPFFEPLDDQSSDDDADVGEWGDFS
                     FVESASAPVDATDDGDAADVTTDRQVARERAAGGPR"
     gene            2605..3918
                     /locus_tag="VH419_06000"
     CDS             2605..3918
                     /locus_tag="VH419_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020384797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEY7043204.1"
                     /translation="MIDHDLVRQLRVQVADRLNEQRRRDQMNGVAPMSPEDEREYSRS
                     LIIQILEDHARMEIADGRTPPTPEEEAGLASAIHSALFGVGRLQPLLDDPEVENVDIN
                     GCDNVFVQYGDGREERHGDVAESDEELVELIQVLAAHTGLASRPFDSANPQLDLRLPD
                     GSRLSAVMNVCARPSVSIRKARLGRVFLDDLVVNGTMTPELGEFLKAAVAARKNIMIA
                     GSTNVGKTTLLRALANEIPSWERLITVERALELGLGEFADLHPNVVAFEERLANSEGV
                     GGVSMAELVRRSLRMNPSRVIVGEVLGDEIVTMLNAMSQGNDGSLSTIHANSSLEVFN
                     RISTYAIQASERLPIEATHMLIAGALDFVVFMRKRNEYADGGGLSRIVESVREVVGHD
                     QRVLSSEVFGPDPTTGVAVPHAPIQCIDDLARNGYRPLVSGRGWN"
     gene            3918..>4606
                     /locus_tag="VH419_06005"
     CDS             3918..>4606
                     /locus_tag="VH419_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020384796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEY7043205.1"
                     /translation="MPFDTLLVALTLGAVVGTGAFLLVLFFVGHERGGSEVTPPSRFD
                     LKQVDFRSGTLHAALGAVTFFAVMAATQWIVLAIAMGLLVAFWKRIFGGAAEERRAIE
                     RVEGLTAWTEALRDTIAGAVGLEQAIPATAVNAAPVIRPSLNLLVDRLRVREPLPSAL
                     MKFADDLDDPSADLIIAALILNSRLRGPGLREVLSALSESGREELDLRRRVEGSRRST
                     RRSVQIVVGVTL"
BASE COUNT          752 a         1606 c         1558 g          690 t
ORIGIN      
        1 tcgcaaacct ccgatgggca gagggggtct cggatgtgga catcgcaaac atcagccagc
       61 tcagccacgg atgtccgaaa ctcgccactg actcaccggg ccacaggaac ggaatgccgc
      121 tgactcacga atcgggcagg ctttggtggt ggtttgcctc ggtagccatg caaacggacg
      181 ggtagccagg cagatctgcc ttgggagggg tgcatgcgcc tcgtgaccga ggcaaaccgg
      241 gaccggcgag cgacgtccga aacccgccag tgacacacca agcaaagggg gccggatggc
      301 ggccgaccgt ctgagtgggg cacgaacggt tcccgctctt cgctgcccac actcctcaac
      361 ccgcgcggac gctgcgtcgg gcgaccacat tcgcactgcg cgaccatctc cgtcccacgg
      421 aagcctgcgc tgccaccaga cctgggacct gcaaccgctg ggtttgctgg cccgcggatg
      481 atcgaagcca cgcgccggcg aagtccgaaa gcgacccgtc acacacccac cgaaaggaac
      541 cggacggcgg ccgatcgcct cagcgggcag gaacgcttcc cgcacttcgg tgccgcctca
      601 cctcagccca cccggacgta cgccgggcga ccccgcggac caatgccgac cagctccgcc
      661 cacggccagc ccagggtcaa tccgcacgaa ggtgcgccgc gtgatcaaga tcacccgacg
      721 tgaccacgtc cgactccaga agcctcagga gcgcccccga tctgtctggc gaaccgtcca
      781 ttcggatggc taccgaggca aaccgtcccg tcagcaggcg cgaccgcggg acaacagccg
      841 gggctctgaa ttccggaacg attcgcgctg acccgtcagg aatcgtagag tttgacccgc
      901 accagccgcg caactcgggt tttcgcgcgg ccagaaacga tcggacgcca ccttcatgac
      961 catcacgaac cagccgccgc cgggaagccg acgggggcgg tccaccccac cgccgatgcc
     1021 caccaggctt ccgtcgacgc gggaacgccg gccggcgttg gccgcgctcg cggtcctgct
     1081 catcgtcggc ggtgcggtgc tggccggctg gttcgcgctc cgccagagcc agacggagtc
     1141 gtacctggtc atcgacgaga cggtccacac cggcgagaag atccagccta gcgacctgcg
     1201 agagatcgag ctgccggccg acagcgggga cctcattccg gccagccagg cagacgacgt
     1261 gatcgactcc tacgcgcagg tggacctgct gcaggggacc gtggtcgccg ctgggatgta
     1321 cggcgacgct cccgagctcg ccgccgacga ggacctcgtg ggcctcgacc tcgacccgtc
     1381 ccagttcccg aagtcgatcc accccgggga ctccgtagtc gtcgtcctga gcgctgccga
     1441 ggggagtacg ccgatcctgc tgtccgacgg caccgtccgg gcgatcaagg gcgcagagac
     1501 cggtggcggg tcgaccgtcg acgtggtgat ctcgaaccag tgctccgacc agatggccac
     1561 cggttcctcc cacggtgatg tctcgctcgt cgaggtggcg ccgaacgcac gtgcgatcac
     1621 ctgcgcctct ccggaccagg tcgccaagct gcccaagggg aacccgtaaa tggcccttgt
     1681 gacggtggcc gccgcgaagg ggtcgcccgg ggtcacgacg accacgctgg tcttgggcgc
     1741 actctggccc cgccaggtgc tcgtcgccga gtgtgacgtt gcgggggcgg acattccgtt
     1801 gcgtatgtcg gccgccgaca gcggtgtgct cgacgccgac cgcggactgc tgagcctggc
     1861 ggccgccggg cgcaagggcc tgaattccga tctcgtcctc acgcacagcc agcaggtgct
     1921 cggtggtatg gacgtgctgg tgggaccacg cgtgccggag caggctgccg gtctcacgaa
     1981 cctgtggccg atgctcggtc cggcgctcga cgggattccg gggtacgacg tgatcgccga
     2041 ctgcggccgg atcggggctg acacaccaca gaacgtcctg ctccgggcgt ccaggctgct
     2101 cgtcctcgtc tgcacggtcg agccgagttc tgtcgtccac ctgcgcgagc ggctcacgac
     2161 gctcgcaccg atgctcgacc cgtcctcacc ggtcggtacg ccgatcgcgg tcgtcgtcgt
     2221 cgccgacccg aaggcccgcg acgcggtcgc gcaggcgcgc cagtcgctcg aacgcaccga
     2281 gatccccctg cagggcgtct ggcacctcgc gtacgacccg aagggtgcag ggttcttcaa
     2341 gggtcacgtg gtcggtcgcg ccgacaagac gatgctcgtc aagaccgccc gcccagcggt
     2401 cgagcaggtc gcggcactgg tggagccgtt cttcgagccg ctggacgacc agtcatctga
     2461 cgacgacgcg gacgtgggcg agtggggaga cttctcgttc gtcgagtccg cgagcgcacc
     2521 cgtcgacgcc acggacgacg gcgatgcagc tgatgtgacg acggatcgcc aggtggcccg
     2581 tgagcgggcg gcgggaggcc cgcgatgatc gaccacgacc tggtcagaca gcttcgcgtc
     2641 caggtggcgg accggctcaa cgagcagcgg cggcgcgacc agatgaacgg cgtggctccg
     2701 atgagcccgg aggacgagcg ggagtactcc cggtcgctga tcatccagat cctcgaggac
     2761 cacgctcgga tggagatcgc cgacggtcgt acgccgccga cgcccgaaga ggaggccggc
     2821 ctcgccagcg cgatccattc ggcactgttc ggggttggcc ggctgcagcc actcctcgac
     2881 gaccctgagg tcgagaacgt cgacatcaac ggctgcgaca acgtcttcgt ccagtacggc
     2941 gacgggcgcg aggagcggca cggcgacgtg gcggagtccg acgaggagct ggtcgagctc
     3001 atccaagtgc tcgccgccca caccggcctc gccagccgcc cgttcgactc cgcgaacccg
     3061 cagctcgacc ttcgactccc cgacggctcc cggctgtcgg cggtcatgaa cgtctgcgcg
     3121 cgcccctctg tctcgatccg caaggcgcgg ttgggcaggg tgttcctcga cgacctggtc
     3181 gtcaacggca ccatgacgcc agagctcgga gaattcctga aggcggccgt cgccgcgcgc
     3241 aagaacatca tgatcgccgg ctcgaccaac gtcggcaaga cgacgctgct gcgcgcgctg
     3301 gccaacgaga ttccctcgtg ggagcgactg atcacggtgg agcgggcgct ggagctcggg
     3361 ctcggcgagt tcgcggacct gcacccgaac gtcgtcgcgt tcgaggagcg gcttgccaac
     3421 tccgaaggcg tcggcggcgt ctccatggcc gagctcgtcc gccgctcgct gcggatgaac
     3481 ccctcccgcg tcatcgtcgg cgaggtgctc ggcgacgaga tcgtgaccat gctcaacgcg
     3541 atgagccagg gcaacgacgg gtcgttgtcg acgatccacg cgaactcctc gctcgaggtc
     3601 ttcaaccgga tcagcaccta cgcgatccag gcctccgaac ggctgcccat cgaggcgacg
     3661 cacatgctga tcgccggtgc cctcgacttc gtcgtgttca tgcgcaagcg caacgagtac
     3721 gccgacggag gcggtctcag ccggatcgtc gagagcgtcc gagaggtcgt cgggcacgac
     3781 cagcgggtcc tctccagcga ggtcttcggg cccgacccca cgacgggtgt cgcagtgccg
     3841 cacgcgccga tccagtgcat cgacgacctg gcgcgcaacg gctaccgtcc gctcgtctcc
     3901 ggccgcgggt ggaactgatg ccgttcgaca cgctcctcgt cgcgttgacg ctcggcgctg
     3961 tcgtcgggac cggcgccttc ctgctcgtgc tgttcttcgt cggccacgaa cgcggcgggt
     4021 ccgaggtgac accgccgagc cgcttcgacc tgaagcaggt cgacttccgc agcggcaccc
     4081 tccacgcggc gttgggtgcg gtgacgttct tcgcggtcat ggccgctacg cagtggatcg
     4141 tgctcgcgat cgcgatggga ctgctggtgg cgttctggaa gcggatcttc ggcggagccg
     4201 ccgaagaacg ccgcgccatc gagcgggtgg aagggttgac cgcgtggacc gaggcgctgc
     4261 gcgacacgat cgccggcgcc gtcggcctgg agcaggcgat cccggcgacg gcggtgaacg
     4321 cggcgccagt catccggccg agcctcaact tgctggtcga ccggctgcga gtccgcgagc
     4381 cacttccgag tgcgctgatg aagttcgccg atgacctgga cgacccctcg gccgacctca
     4441 tcatcgcggc gctgatcctg aactcccggc ttcgtggtcc tgggctgcgc gaggtgctca
     4501 gcgcgctgtc ggagtccggc cgtgaggagc tcgacctgcg cagacgggtc gaaggctccc
     4561 gacggagtac gcgtcgcagc gtgcagatcg tggtcggcgt caccct
//
LOCUS       DATBFZ010000197         1526 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_2751715, whole genome shotgun sequence.
ACCESSION   DATBFZ010000197 DATBFZ010000000
VERSION     DATBFZ010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2751715"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /locus_tag="VH419_06010"
     CDS             <1..561
                     /locus_tag="VH419_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY7043206.1"
                     /translation="AQTGTYDVIVLDIMLPGLNGYDVCRTLREQENWTPVIMLTAKDG
                     DLDQVDALDLGADDYLTKPFAFAVLLARLRALVRRGAPARPAVLVAGDLRLDPASRRV
                     ARGEEAITLTPREFGLLEFLMRRAGEVVSKTDILENVWDAYYQGDDNVVEVYVGYLRR
                     KIDQPFDRRAIETLRGMGYRLAADGG"
     gene            671..>1526
                     /locus_tag="VH419_06015"
     CDS             671..>1526
                     /locus_tag="VH419_06015"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEY7043207.1"
                     /translation="MVLVVAGVSLVLVQRHLLRDGIVDLAQRQASELAAQVEVQGIRS
                     PELEALVQSEDGVVQVVDPEGRVVAASGSISGSAPIVDVRPSDGETATVFTDGLPGGH
                     DDPFAVVARGVSTPDGTAVVVSAESLESAQEATSTLVSVLAVGIPIVVLIVGGLSYWL
                     TGRALAPVRALRARVAEIHAGDRSARVPVSPAGDEISRLAETMNAMLERLQAASAQQR
                     RFVADASHELRSPLAAIRTAHEIGLAHPESTDWPGTSRDTLTELERLEKLVADLLLLA
                     RFDDNEPGT"
BASE COUNT          226 a          526 c          535 g          239 t
ORIGIN      
        1 gcgcaaaccg ggacgtatga cgtcatcgtg ctcgacatca tgttgcccgg cctcaacggg
       61 tacgacgtgt gccgcaccct gcgcgagcag gagaactgga cacccgtcat catgctcacc
      121 gccaaggacg gggacctcga tcaggtcgac gcactcgacc tcggcgccga cgactacctg
      181 acgaagccgt tcgcgtttgc cgtgctgctg gcccggctgc gtgcgctggt acgccgtggc
      241 gcccctgcgc gccccgccgt cctcgtcgcc ggcgacctgc ggctcgaccc tgcctcccgc
      301 cgggtggcac ggggcgagga agccatcacg ctgacgccgc gggagttcgg gctgctcgag
      361 ttcctgatgc gccgggccgg cgaggtcgtc agcaagaccg acatcctcga gaacgtgtgg
      421 gacgcctact accaaggcga cgacaacgtc gtcgaggtgt atgtcggcta cctgcgtcgc
      481 aagatcgacc agccgttcga ccgacgcgcg atcgagacac ttcgcggcat gggctaccgg
      541 ttggcggccg acggtggcta gaacgggggt acgccgtcga cccaggaggc aacctctcga
      601 gcaatcgcgt gtccgttggt tggccggagt gcgtgtccgc accactttgg ccgctacatg
      661 cgccgcagcc gtggtgctgg tggtcgccgg tgtgagcctg gtcctggttc aacgccacct
      721 gctgcgcgac ggcatcgtcg acctcgcgca gcggcaggcg tccgagctcg cggcccaggt
      781 cgaggtgcag gggatccggt cgccagagct cgaggcgttg gtccagagcg aggacggcgt
      841 cgtccaggtc gtcgaccccg agggcagggt ggtggctgcc agcgggtcga tttcgggatc
      901 cgcgccgatt gtcgacgtgc gcccgtcgga cggggagaca gcgacggtct tcaccgacgg
      961 gctgccaggc ggacacgacg acccgttcgc tgtcgtcgcc cggggcgtgt ccacgccgga
     1021 cggcacggca gtggtggtgt ccgccgagag tctcgagagc gcccaggaag caaccagcac
     1081 gctcgtctcc gtgttggccg tcggcatacc gatcgtcgtg ctgatcgtcg gtggactgtc
     1141 gtactggctc accggtcgcg ccctcgcgcc ggtgcgagcc ctccgcgcca gggtggccga
     1201 gatccacgcc ggcgacaggt cggcccgggt gcccgtctca ccggccggtg acgagatctc
     1261 ccggctcgcc gagaccatga acgcgatgct cgagcggctc caggccgcgt cggcccaaca
     1321 gcgtcggttc gtcgctgacg cctcccacga gctgcgcagt ccgcttgcgg cgattcgcac
     1381 cgcccacgag atcggactcg ctcacccgga gtcgacggac tggcctggta ccagtcggga
     1441 cacgctgacc gagctggaac ggctggagaa gctcgtggca gacctgctcc tgcttgcccg
     1501 cttcgacgac aacgagcccg ggacgg
//
LOCUS       DATBFZ010000198         3128 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_4065066, whole genome shotgun sequence.
ACCESSION   DATBFZ010000198 DATBFZ010000000
VERSION     DATBFZ010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4065066"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..673
                     /locus_tag="VH419_06020"
     CDS             <1..673
                     /locus_tag="VH419_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008357349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEY7043208.1"
                     /translation="NAMATMKCDMSGAAAVSAATFAIAELGLPVRVTAYACLAENMPS
                     GHATRPGDVLTMYGGKTVEVLNTDAEGRLVLADGITMANADKPDVIVDVATLTGACVV
                     ALGNKVSGVFSNDDTLHRELPKVAARAGEHMWPMPIPEEMKEKLRTTKVADLSQHNPE
                     QWGGALFAAAFLREFVADGISWAHLDIAGPAFNDGTPSGYTPKGGTGTAVRTLVQLAR
                     ERATG"
     gene            complement(781..1122)
                     /locus_tag="VH419_06025"
     CDS             complement(781..1122)
                     /locus_tag="VH419_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020387638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043209.1"
                     /translation="MGFLRRRRNKPGTLRAAESADATYLKDWIASRRGIEGFIEPRTT
                     VTDTTILLVAIDGEWTRRRVPSVEWAHNFANRNGIPSYDAAVVGYPDRMREYNKRQKG
                     GTSGPTSGPDS"
     gene            1403..2788
                     /gene="lpdA"
                     /locus_tag="VH419_06030"
     CDS             1403..2788
                     /gene="lpdA"
                     /locus_tag="VH419_06030"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009074768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEY7043210.1"
                     /translation="MTDAESRFDILILGGGSGGYACAIRAAELGMNVGLVEQDKVGGT
                     CLHRGCIPTKALLHAGEIADSVRDAGTFGITATLDGVDLVGVNAYKDGVVARLYKGLE
                     GLIKGHGITVIEGRGTLAAANAIDVDGTRYEASHIVLASGSYTKSLPGLDLDGERVIG
                     SWDALQLESVPNRAIVLGGGVVGVEFASAWRSLGADVTIIEALPRLVPAEDAACSKAV
                     ERAFRKRGIVFKTGTAFASLDKTDDGVRVTVESGDTFEADVLLVAVGRGPNTADLGYE
                     VHGVTMDRGFVRTDDRLQTSVPGVYAVGDIVPGMQLAHRGFQHGIFVAEEVAGLRPAA
                     IDEAGIPRVTYCDPEVASVGLTEEDAKAAYGEDEVQTLTYDLAGNGKSQILKTQGFVK
                     LVRRKDGPVLGLHMVGARVGELVAEAQLIYNWEAFPEDVAGLVHMHPTQSEALGEAHL
                     ALAGKPLHTHA"
     gene            3030..>3128
                     /locus_tag="VH419_06035"
     CDS             3030..>3128
                     /locus_tag="VH419_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007078131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEY7043211.1"
                     /translation="MATDVTLPALGESVTEGTVTQWLKSVGDEVAVD"
BASE COUNT          527 a         1051 c         1064 g          486 t
ORIGIN      
        1 caacgcgatg gcgacgatga agtgcgacat gtccggggcg gccgcggtgt cggccgccac
       61 gttcgccatc gccgaactcg ggctgcccgt gcgggtgacg gcgtacgcct gtctggccga
      121 gaacatgcca tccggacacg ccacgcgacc cggggacgtg ctcacgatgt acggcggcaa
      181 gacggtcgag gtgctcaaca ccgatgcgga agggcggctc gtcctcgccg acggcatcac
      241 catggcgaac gccgacaagc ctgacgtgat cgtcgacgtc gcgacgttga ccggcgcctg
      301 tgtcgtggca ctgggcaaca aggtgtccgg ggtgttcagc aacgacgaca cgctgcaccg
      361 agagctgccg aaggtcgccg cgcgggcggg tgaacacatg tggcccatgc cgattcctga
      421 ggagatgaag gagaagctcc ggacgaccaa ggtcgccgat ctcagccagc acaaccccga
      481 acagtggggt ggcgcgctgt tcgctgcggc gttcctgcgc gagttcgtgg ccgacgggat
      541 cagctgggcg catctcgaca tcgccggccc ggcgttcaac gacggcacac ccagcggcta
      601 cacgcccaaa ggtggcaccg gcaccgccgt ccgcaccctc gttcagctgg ccagggagcg
      661 agccaccggc tgacgcccga cggggcttcc gcgaattgcg gtgaagtggc tgcgttcgag
      721 cgccgcacag cctgaccttt cccgcaattc gcggggacca ctgaccagcc gaggctgagg
      781 tcaggagtcg gggccggagg tggggccgga ggtgccgcct ttctggcgct tgttgtactc
      841 gcgcatccgg tcggggtatc cgacgacggc ggcgtcgtag ctcgggatgc cgttgcggtt
      901 cgcgaagttg tgcgcccact ccaccgacgg aacccgccgc cgggtccact caccgtcgat
      961 ggccaccagc aggatcgtcg tgtcggtgac ggtcgttcga ggctcgatga aaccctcgat
     1021 gcccctccgg gacgcgatcc agtccttgag gtacgtggcg tctgccgact cagcggctcg
     1081 cagcgtcccc ggcttgttgc gtcggcgccg cagaaacccc atcaacgtcc ctctccacgg
     1141 tggtttcgtg tgtgaacaag tatcgcgcac cggcccaaca ccgacggcat ttcgaggtgc
     1201 agcggctgcg tggaccgtac tcgggcaacg gcacgccgtc tggctgggcg catccgaacg
     1261 gctggtgcaa ggatggccgt tggtctcgta cgtcgtctcc cgcgtccggt gagtgacgcg
     1321 cgctcacgac tcgaccccgc cgtcaaccga acgtcaaagc aacgtcaaag gaacgtcaaa
     1381 ggaacactgt gaaaggcaca ccgtgacaga cgccgaaagc cggttcgaca tcctcatcct
     1441 cggcggtggc agcggggggt atgcgtgcgc catccgcgca gccgagctcg ggatgaacgt
     1501 cggcctcgtc gagcaggaca aggtcggtgg gacctgcctg caccgggggt gtatccccac
     1561 caaggcgctc ctccacgccg gcgagatcgc agactccgtc cgggatgccg gcacgttcgg
     1621 catcaccgcc accctcgacg gagtcgacct cgtcggggtc aacgcctaca aggatggtgt
     1681 cgtcgcccgt ctgtacaagg ggctcgaagg cctgatcaag ggccatggga tcaccgtgat
     1741 cgagggccgc ggcacgctgg ccgccgccaa cgccatcgac gtcgacggca cccgctacga
     1801 ggcgtcccac atcgtgctgg cgtccggctc gtacacgaag tcgctgcccg ggctggacct
     1861 cgacggcgag cgcgtgatcg ggagttggga cgcgctccag ctcgagtcgg tgcccaaccg
     1921 ggccatcgtg ctgggcggcg gtgtggtggg cgtcgagttc gccagcgcct ggcgttcgct
     1981 cggcgcggac gtcacgatca tcgaggcgct tccccggctc gtgcccgccg aggacgcggc
     2041 ctgttccaag gcggtcgagc gcgcgttccg gaagcgcggc atcgtcttca agaccggcac
     2101 ggccttcgcg tcgctggaca agaccgacga cggcgtacgc gtgaccgtcg agtccgggga
     2161 caccttcgag gcggacgtgt tgctggtcgc ggttggccgg gggccgaaca cggccgacct
     2221 cggatacgag gtgcacggcg tgacgatgga ccgcggtttc gtccgcaccg acgacaggct
     2281 ccagaccagc gtccccgggg tgtacgcggt cggcgacatc gttccgggaa tgcagctggc
     2341 gcatcgtggc ttccagcacg gcatcttcgt cgccgaggag gtcgccgggc tccgacctgc
     2401 tgcgatcgac gaagccggca ttccccgggt cacctactgc gaccccgagg tcgcctcggt
     2461 cggcctcacc gaggaggacg ccaaggcggc gtacggcgag gacgaggtgc agacgttgac
     2521 gtacgacctc gccggcaacg gcaagagcca gatcctcaag acacaagggt tcgtgaagct
     2581 cgtacgccgc aaagacggcc cggtgctcgg actgcacatg gtcggcgcac gagttggtga
     2641 gctcgtcgcc gaggcacagc tcatctacaa ctgggaggcg ttccccgagg acgtggcagg
     2701 actcgtccac atgcatccga cacagagtga ggcactcggc gaggctcatc tcgctcttgc
     2761 aggcaagccg ctgcacaccc acgcatgacg agcgcctcgt cctcagatga cctagtgccc
     2821 gtcgaccgac attcgccctg ttgcgcgtcg cctgagcggc gatctgctgc gttctcgtcg
     2881 tcgcccgatg ctgggcatcg atctcctcct cggccttgca tctcatcccc tcggacgccg
     2941 ctcacgacga gcaacgccgg tcgacgtggc actaggctga cgcttgcgac gtacgaccgg
     3001 aactctcgac gaaggggacc ccgagcgaca tggcgacaga cgtaaccctc cccgcgctcg
     3061 gtgagagcgt caccgaaggc acggtcaccc agtggttgaa gtcggtcggc gacgaggtgg
     3121 ccgtcgac
//
LOCUS       DATBFZ010000199         3384 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_5973157, whole genome shotgun sequence.
ACCESSION   DATBFZ010000199 DATBFZ010000000
VERSION     DATBFZ010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 3384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3384
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5973157"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(154..534)
                     /locus_tag="VH419_06040"
     CDS             complement(154..534)
                     /locus_tag="VH419_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043212.1"
                     /translation="MVDLEGGPAATHGSSRHLVGAARVDAVHAGAPASPAETVRNWRY
                     QFDADAAAGRDLTVGLHFTDSGEKVTACVRHQILVVQDGIADSCDAVVELTLPTSTDP
                     PTSPSCRATPRSGRCAARHRRPMV"
     gene            675..2552
                     /locus_tag="VH419_06045"
     CDS             675..2552
                     /locus_tag="VH419_06045"
                     /inference="COORDINATES: protein motif:HMM:NF016693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 7-halogenase"
                     /protein_id="HEY7043213.1"
                     /translation="MVTNRYEVAILGSGIGGSMLACILARHGVSTLLLEGGTHPRFTI
                     GESLIPETGIRLRIVGEKYGVPEIGWIGSFHALRDNVSSSCGVKRSFSFMYHSAGAHH
                     RPEQINQLPTLTPPIGPDSHLFRQDTDAYLAALSVQYGATLRSQTRVEDIVFYDDEVE
                     LRATSGDTYRAKFLIDASGMRSILSDRLGMRDETPRFRTDTRALYTHMMNVKSADLLL
                     ERPTRRGVPSPLGQATMHHVFDGGWIWVIPFNNHRDATNPLTSVGMMLDRRKHPNPPG
                     TPEQEFRRIISKYPTVARHFADATAARPWIGTGRIQYSSPSLIGPRLLQLPHAGAFVD
                     PLYSSGMSVLIVAVDLIAEALLGAVADNDYATDRFRFIDEVVNRSFDHYDMIVSRSFD
                     AFASYDTWNAWNRNWALGNMLGAFGPLSLLLRYLRTKDRTHLEKTTEPGRIGVLGSHL
                     PDVVTTMRASQDDIDAATQGRITHAEAGRRIFDRLGELDFVPGYMGFGDPTQGATATF
                     TLPAGARHVTWYRMHGSEKWRENCTFPLTTYARETLRFMAGEARAAWRRGTSAMRDVF
                     FASNDDWRRTPPALAAHGKLWSPIPMTVHLDEELIPAEPLGESSGNGIPLTDTKSTAS
                     T"
     gene            2664..>3384
                     /locus_tag="VH419_06050"
     CDS             2664..>3384
                     /locus_tag="VH419_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043214.1"
                     /translation="MTIRERLRRISPAILTIAVATSLYVAAQNAVATTGVSQTAARYK
                     FHEMPIAMPPGYDRQETKTVREVNPAYHKIRSWISAVGASIALNDVTGHGRPNGMCIV
                     DTRTNQVIVTYAPTAPQADRFTPFVLDPAPLPYDDAMAPTGCTPGDFLGDGRMGFLVT
                     YWGRTPIAFLPKSNAPTPSSSAYVPQEVMQSASLNGTYNGPRWNTDAAYVGDLDGSGH
                     PSIIIGNYFPDSDVLDTAGLKN"
BASE COUNT          615 a         1183 c         1053 g          533 t
ORIGIN      
        1 tcgcccaccg gcgcctcggt gcggatgacg gccgaggtga ccggacgcgg cctgcgccgg
       61 ccgctggcag cggtcgtcac ccgggagatg cgcgcctcga ccctgtcggc cctggcagcg
      121 ctgcggtcac ggttggcccc acgctgagcc gcgtcagacc atcggccgcc gatggcgtgc
      181 ggcacaccga cccgaccgtg gcgtcgcccg acatgatggt gacgtaggag ggtccgtcga
      241 ggttggcagg gtgagctcga cgaccgcgtc gcaactgtcg gcgatgccgt cctgcacgac
      301 caggatctgg tggcgcacgc aggcggtgac cttctcgccg gagtcggtga agtgcaggcc
      361 gacggttagg tcacgaccgg cggctgcgtc ggcgtcgaac tggtagcgcc agttccggac
      421 ggtctcggcc ggacttgccg gcgcgccagc atggacagca tcgactcggg cagccccgac
      481 caggtgccgc gacgagccgt gcgtcgcagc cggaccacct tcgaggtcaa ccatttcgtg
      541 gccgatgtta tcggccgatc acggcaacgc cagagaagtt gtgccacaca ccaaacgagc
      601 atgctgaagg cggcaggcgg ttcggttctg tggaagaaca caaccgtcaa atactccacg
      661 aaacatgggg agctatggtc accaaccgat atgaagtggc gatcctcggc tcgggaatcg
      721 gtggctcgat gttggcgtgc attctcgcca ggcacggcgt ctcgacgctg ttgctcgagg
      781 gaggcaccca tcccaggttc accatcggcg agtcgcttat tccagagacc gggatccgac
      841 tgcgcatcgt cggcgaaaag tacggcgtgc cggagatcgg ctggatcggc tcgttccacg
      901 cgttgcgtga caatgtcagc tcgagttgtg gggtaaagcg ctcgttcagc ttcatgtacc
      961 acagtgccgg cgcgcaccat cggcccgagc agatcaacca actgccgacg ttgaccccgc
     1021 cgatcggtcc tgactcgcac ctgttccgcc aggacacgga cgcgtatctc gcagcactct
     1081 cggtgcagta cggcgcgact ctccggtcgc agacacgcgt cgaggacatc gtcttctacg
     1141 acgacgaagt cgaactgcgc gccacgtcgg gtgacaccta ccgcgccaag ttcctcatcg
     1201 acgcctccgg gatgcgctcc atcctctcgg accggctcgg catgcgggac gagacgccgc
     1261 ggttccgcac cgacacccgc gccctctaca cccacatgat gaacgtgaag agcgcggacc
     1321 tcctgctgga gcggccgacc cggcggggag tgccatcgcc cctcgggcag gcgaccatgc
     1381 accacgtctt cgacggcggg tggatctggg tcatcccgtt caacaaccat cgcgacgcca
     1441 ccaacccgct caccagcgtg ggcatgatgc tcgaccggcg caagcacccg aatccaccgg
     1501 gcaccccgga acaggagttc cggcggatca tctcgaaata cccgaccgtc gcgcggcact
     1561 tcgccgacgc gaccgctgct cgcccgtgga tcgggaccgg ccgtatccag tactcatcgc
     1621 cgagcctgat cggaccccgg ctgcttcagc tgccgcacgc gggagcgttc gtcgacccgc
     1681 tctactcgag cggcatgtcg gtgctcatcg tcgcggtgga tctcatcgcg gaggcgctgc
     1741 tcggcgccgt cgccgacaac gactacgcca cggacaggtt ccggttcatc gacgaggtag
     1801 tcaaccggag cttcgaccac tacgacatga tcgtgtcgcg gtcgttcgac gcgttcgcga
     1861 gctacgacac ctggaacgcg tggaaccgca actgggcact cggcaacatg ctgggtgcgt
     1921 tcgggcccct gtcacttctg ctcagatacc tgcgcaccaa agaccgtacc cacctggaga
     1981 agaccaccga gcccgggcgg atcggcgtgc tcggcagcca tctgcccgac gtcgtcacga
     2041 ccatgcgggc atcgcaggac gacatcgacg ccgccaccca aggccggatc acgcacgccg
     2101 aagccggccg caggatcttc gaccgcctcg gcgagctcga cttcgtgccc ggatacatgg
     2161 gtttcggcga tcccacgcag ggggcaaccg cgaccttcac cctgcccgca ggggctcggc
     2221 acgtcacctg gtaccgcatg cacggttcgg agaagtggcg cgagaactgc acgttcccgc
     2281 tgaccaccta cgcccgcgag accctgcgct tcatggccgg ggaagcgcgc gccgcgtggc
     2341 gtcgcggcac atccgcgatg cgcgacgtgt tcttcgcatc gaacgacgac tggcgccgca
     2401 cgccaccggc gctcgccgcg cacgggaagc tgtggtcgcc gatcccgatg accgtccacc
     2461 tggacgagga actcatcccg gccgagcccc tcggcgagag ctccggcaac ggcatcccgc
     2521 tgacggacac caagtcgaca gcgagcactt gaggcatcgc ttctcgattt gcacacacct
     2581 gactcctgtc aacgatcggc aacacctcct gaacgctcgc gggtttttcg ggatcgttca
     2641 cgcgtcgaaa ggaaaccgtg gtagtgacga ttcgtgagcg cctccgcagg atctctccgg
     2701 caattctgac tattgccgtc gccacgtcgc tatatgtggc ggcgcaaaac gcggtcgcga
     2761 cgacaggtgt cagccagacc gccgcgaggt acaagttcca cgagatgccc atcgccatgc
     2821 cgcccggata cgaccggcag gaaaccaaga cggtaaggga agtcaacccg gcgtaccaca
     2881 aaattcgttc gtggatatcg gccgtcggcg cgagcatcgc cctcaacgac gtcacgggac
     2941 acggccgccc gaacggaatg tgcatcgtcg acactcgcac gaaccaggtg atcgtgacct
     3001 atgcccccac cgcacctcag gccgaccggt tcaccccatt cgtcctggac ccggcaccgc
     3061 tgccgtacga cgacgcgatg gcgccgaccg ggtgcacgcc cggcgacttc ctcggtgatg
     3121 gccgcatggg cttcctggtg acctactggg gacggactcc gatcgcgttc ctgccgaagt
     3181 ccaacgcgcc cactccgtca tcgagcgctt acgtgccaca ggaggtgatg cagtccgcgt
     3241 cgctgaacgg cacgtacaac ggcccgcgct ggaacaccga tgccgcatac gtcggcgacc
     3301 tcgacggcag tggacaccct tcgatcatca tcggcaacta cttcccggac tccgacgtgc
     3361 tcgacacggc cggcctgaag aacg
//
LOCUS       DATBFZ010000200         3042 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_1440169, whole genome shotgun sequence.
ACCESSION   DATBFZ010000200 DATBFZ010000000
VERSION     DATBFZ010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 3042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3042
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1440169"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="VH419_06055"
     CDS             complement(<1..267)
                     /locus_tag="VH419_06055"
                     /inference="COORDINATES: protein motif:HMM:NF023880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose acetyltransferase domain-containing
                     protein"
                     /protein_id="HEY7043215.1"
                     /translation="MSDNLRRLAEGDWYITDEAVLDLQRERQALMERYNAATIADPST
                     RRALLEELMGEVGEEVEVRSPVYVDYGSNVRIGSGVFVNYGCQLA"
     gene            complement(357..1286)
                     /locus_tag="VH419_06060"
     CDS             complement(357..1286)
                     /locus_tag="VH419_06060"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020579458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEY7043216.1"
                     /translation="MSWQPWREAWRQALYGPAGFYLHAEPQQHFRTSVHASPLFAASI
                     LRLVRRSGLSAVTDYGAGSGELLTQLAHLAPDLVLTGIDLRPRPIDLPASIGWRHELP
                     DDISGLLFANELLDNVPCQVVELDPRGVVRIVEVDSVSGAERLGSEAADAETRWLDDW
                     WPLELAGDRAEVGLDRDEFWEDACGRVVSGMSLALDYGHLQADRPTTTTLRSYRLGRE
                     TPVRLDGTADVTAHVAVDSVAAAVRASVRRQRDMLHELGVSAIRPPLPLASTDPAAYV
                     RGLAQASQAAELVDSPGLGDLYWLLAEHSTDTG"
     gene            1364..2947
                     /locus_tag="VH419_06065"
     CDS             1364..2947
                     /locus_tag="VH419_06065"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY7043217.1"
                     /translation="MTAWRRARTAPRLSVVVPVYNVEDYLAECLDSVLAQTYRENDEI
                     EVILVDDGSTDGSAEIARQYADRHATWKLVRTANHGLGAARNRGAQEAGGAYLAFVDS
                     DDMVPADAYALMIATLDRSGSDFVVGSVHRLIDGRLVEPEFLKPGVARRRIGVRAADV
                     PPIIRNVFAWNKVFRRSFYDSAGLRFPEGIRYEDQPTTMRAYLLADRFDIVRRPVYIW
                     RVRGERSSITQGRGQLSDLEDRLKTKALTSALVRTHGDEAVVDYWGKLALLGDMPVYF
                     AEIATADDAYWARLVSGLREMLDGFPPIEQSLLRLPQRLVGWLVVQDRRGDAERVLGW
                     LHDHPGPLSLRVEGDHVVPLDLPVASDPESGVPDGVRRLGEHELEFDARLVDVHWDGS
                     ALVVTGWALVRGAPSAGVSTDIRAELRGGDEAGGARVAADVERFEAAEATRWVGRGNQ
                     RYDDSGFTARFDLGGWSPGPHGAGDALTLWVEVEVDAIHRAGRLRSTAPELDPARLPE
                     TTFARVEWRPDQGLTVVRR"
BASE COUNT          464 a         1031 c         1064 g          483 t
ORIGIN      
        1 ggccagctgg cagccgtagt tcacgaacac accgctgccg atgcgcacgt tcgacccgta
       61 gtcgacgtac accggactgc ggacctccac ctcctcgccc acttcaccca tcagctcctc
      121 gagcagggcg cgtcgggtcg acggatcagc gatcgtggcg gcgttgtagc gctccatcag
      181 ggcctgccgt tcgcgctgga ggtcgagcac ggcctcgtcc gtgatgtacc agtcgccttc
      241 ggccaatcgc cgcaggttgt cgctcatctg cccaggctag gagggtgctg aaggatccgc
      301 tcgtcgcagc agagcatgat tcttcagcag tctccttgga cgtgccgagc cgccggtcag
      361 ccggtgtcgg tcgagtgttc agccagcagc cagtagaggt cgccgaggcc tggcgagtcg
      421 acgagctccg ctgcttgcga cgcctgtgcg aggccgcgca cgtacgccgc cggatcggtt
      481 gacgccaacg gcagcggcgg ccggatcgcc gagacgccga gctcgtgcag catgtcgcgc
      541 tgacggcgca ccgacgcccg caccgctgcc gcgacggagt cgaccgcgac gtgcgcggtc
      601 acatcggccg taccgtccag ccgaacgggt gtctcgcggc ccaagcggta ggaccgcagt
      661 gtcgtcgtgg ttgggcggtc ggcctgcagg tgcccgtagt ccagcgccag actcatgccg
      721 gagacgacgc gaccgcacgc gtcctcccag aactcgtcgc gatcgagccc cacctcggcg
      781 cgatcacctg cgagctcgag cggccaccag tcgtccagcc atctcgtctc ggcgtcggct
      841 gcttcggaac cgagccgttc cgcaccgctg acagagtcga cctcgacgat ccgtacgacg
      901 ccacgcgggt cgagctccac cacctggcag ggcacgttgt cgagcagctc gttggcgaac
      961 aacagcccgc tgatgtcatc gggcagctca tgccgccaac cgatcgacgc cgggaggtcg
     1021 atcggccgag gccggaggtc gatgccggtc agcaccaggt ccggcgcgag gtgggcgagc
     1081 tgggtcagca gctctcccga accggcgccg tagtcggtga ccgccgacag accggagcgt
     1141 cggacgagcc gaaggatcga tgcggcgaac agcggggagg cgtgcacgga cgtgcggaag
     1201 tgctgctgcg gctcagcgtg caggtagaac ccggccgggc cgtacaacgc ctgccgccac
     1261 gcctcccgcc acggctgcca gctcatcagt gcgaacgtac ccgtccacgg ggcacgtgtg
     1321 cgggccacct cggacggcac cgtcgttgac tacattgatc gccgtgacgg cgtggcggcg
     1381 agcgcggaca gctcctcggc tgagcgtcgt cgttcccgtc tacaacgtcg aggactacct
     1441 ggccgagtgc ttggacagcg tgctcgcgca gacctatcgc gagaacgacg agatcgaggt
     1501 gatcctcgtc gacgacggct cgacggacgg ctcggcagag attgcccgtc agtacgccga
     1561 ccgacatgcg acctggaaac ttgtccggac ggccaaccac gggcttggcg ccgcccgcaa
     1621 ccgtggagcg caggaggccg gcggcgcgta cctcgcgttc gtcgactccg acgacatggt
     1681 cccggcagac gcctatgcgc tgatgatcgc gacgttggac cggagtggct cggacttcgt
     1741 ggtcgggtcc gtgcaccggc tgatcgacgg tcggctcgtc gagcccgagt tcctcaagcc
     1801 gggcgtcgcg agacgtcgaa tcggggttcg agcagccgac gttcccccga tcatccgcaa
     1861 cgttttcgcg tggaacaaag tgttccggcg ctcgttctac gacagcgccg ggctgcgctt
     1921 tcccgaaggg atccggtatg aggaccagcc gacgaccatg cgggcctacc tgctggccga
     1981 ccggttcgac atcgtgcgcc ggcccgtcta catctggcgc gtgcgcggcg agcgcagctc
     2041 gatcacgcag ggacgcggac agctgagcga cctcgaggac cggttgaaga ccaaggcgct
     2101 cacctcagcg ctcgtccgca cccacggtga cgaggcggtc gtggactact gggggaagct
     2161 cgctctgctc ggtgacatgc cggtgtattt cgccgagatc gcgacggccg atgacgcgta
     2221 ctgggcacgg ctcgtgagcg gcctgcgaga gatgctggac ggctttcccc cgatcgagca
     2281 gtcgttgttg cgccttccgc aacgcctggt cggctggttg gtcgtccagg accgccgtgg
     2341 cgacgccgag cgcgtgctcg gctggctgca tgaccatccg ggaccgctgt cgttgcgcgt
     2401 cgagggagac cacgtcgtcc cgctcgacct gcccgtcgcg tccgaccccg agtcgggagt
     2461 tcccgacgga gtacgccggc tgggcgagca cgaactcgag ttcgacgcgc gtctcgtcga
     2521 tgtccactgg gatggctcgg cgctggtcgt caccggctgg gcgttggtgc gtggagcacc
     2581 cagcgcaggc gtgtcgaccg acattcgcgc cgagctgcgc ggcggtgatg aggccggagg
     2641 ggcacgtgtc gctgccgacg tcgagcgatt cgaggcggcg gaggcgactc gctgggtcgg
     2701 ccgcggcaac cagcggtacg acgacagcgg gttcacggcc cggttcgacc tcggcggctg
     2761 gtcgcccggc ccgcacggtg ccggtgacgc cctcacgctc tgggtcgagg tcgaggtgga
     2821 cgcgatacac agagccgggc gcctccgcag caccgcccca gagctcgacc ccgctcggct
     2881 tccggagacg acctttgcgc gagtcgagtg gcgtcctgac caggggctga cggtcgtccg
     2941 aaggtgatgc cccgggatac tgccgtccgt gtgggcggtg aagggtggtt gccggtttcg
     3001 gacatcggtc actaggtgcc cctggttctg cgatgtcccc gt
//
LOCUS       DATBFZ010000201         9287 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_1440263, whole genome shotgun sequence.
ACCESSION   DATBFZ010000201 DATBFZ010000000
VERSION     DATBFZ010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 9287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9287
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1440263"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..692
                     /locus_tag="VH419_06070"
     CDS             <1..692
                     /locus_tag="VH419_06070"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEY7043218.1"
                     /translation="QTVRRLDNSGMARVGAGLDARDAWRAAVVRRHGISFGFLAFNAI
                     GETPRATAEGAGAAEVRMQPRTGPLDAGDLRSATTAVRTLARRVDVVVVLPHWGEQYT
                     NRPVRDQRTVGAALLDAGADMVVGGHPHWVQGIDVHDGKLIVNSLGNFVFDMDFSSQT
                     RQGVLLDVVCWNDKPVSATVTPFLIGADFAPRVVHGPVAATILDRMWATSDPPFRGNR
                     VGETNGTEVDR"
     gene            complement(706..1473)
                     /locus_tag="VH419_06075"
     CDS             complement(706..1473)
                     /locus_tag="VH419_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEY7043219.1"
                     /translation="MRVLAVRFGPREATSTAYQRAADWVAGRFEAYGYAVRRQRLAVP
                     AGISWGVPVPAGTTSNVVATPTDFDAAEPHLVVSAHLDTVPQAPGAEDDASGVGVLLE
                     LARMAASHETRLPAMFVAFAAEEPRGDGEALHHFGSRVMVARMSKPERRAVSGMVSLD
                     RVGVGTVVPLCAGGLLPPHVRAQLVRAGEQAAVLTSRCVDTASDHWSFELAGMPAARV
                     GGTSYAGYHSAADLPRVVNPAQLRRVGVLMWSWLAGR"
     gene            complement(1707..2957)
                     /locus_tag="VH419_06080"
     CDS             complement(1707..2957)
                     /locus_tag="VH419_06080"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020577393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEY7043220.1"
                     /translation="MTKADVHAAARRAREASYVLGTTTRATKDRALRAMADALEHAQN
                     AILSANAEDVSAQESAGTAAALVDRLRLTSERVTGMAEGLRELANLPDPVGEVVRGSM
                     MANGLEMRQLRVPFGVVAMIYEGRPNVTADAAGICLKSGNAVLLRGSSSAARSNTAVV
                     DVLRDAVGSAGLPADVIQLVTGDRDDVGHLMNARGLVDVVIPRGGAGLIDRVVRESRV
                     PVIETGVGNCHVYVDADADLDMAERIVVNAKTQRPSVCNAAESLLVHAAVADEFVPRI
                     VKALATEGVMVHGDEVVRRLSPDVVPATDDDFGTEYLSLDMSVAVVDSGDAAVEHIRR
                     YGTGHTEAIVTRSQEAARRFAAAVDAAAVVVNASTRFTDGGEFGFGAEIGISTQKLHA
                     RGPMGLPEMTSTKYVVIGTGQTRD"
     gene            complement(3131..3481)
                     /locus_tag="VH419_06085"
     CDS             complement(3131..3481)
                     /locus_tag="VH419_06085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043221.1"
                     /translation="MSLPVRLWHVTVTVSGDPQPVSNVHDALSRLQHQQPFLHSIRYD
                     EDKAEIAYWEEGEEMVDAASLALRLWNEHRGSAELPPWKVVGLEVVEQETYQSRPLSS
                     PLSQADTTPRRFSR"
     gene            complement(3584..4705)
                     /gene="proB"
                     /locus_tag="VH419_06090"
     CDS             complement(3584..4705)
                     /gene="proB"
                     /locus_tag="VH419_06090"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013160758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEY7043222.1"
                     /translation="MSAAYGVQALAAAKRVVVKVGSSALTTTEGGIDDHRLRTLVDAL
                     AAKRAGGLEIVLVSSGAIAAGLAPLGLRTRPRDLARQQAAASVGQGLLMHRYTSAFGE
                     HGVKVGQVLLTVDDMTRRSHYRNAYRTFAKLLELGVLPVVNENDTVATGEIRFGDNDR
                     LAALASHLVHADALVLLSDVDAVYDKDPRDPSARRIDVVHGPDEMAHVIVGRRVERSL
                     GSGGMATKLEAARIATGAGIPTVVGHADHIRAVLAGESGTWFAPTGRKRSTRQLWLAH
                     ATEPNGQLVLDNGAVHAVTTRGTSLLPAGVVGVHGRFGAGDPVDLVDTAGQTIGRGLV
                     NYDAAEIPSLLGRTTSELAATFGEGYERELVHRDDLVIL"
     gene            complement(4702..6072)
                     /gene="obgE"
                     /locus_tag="VH419_06095"
     CDS             complement(4702..6072)
                     /gene="obgE"
                     /locus_tag="VH419_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020577388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEY7043223.1"
                     /translation="MAVPTFVDRVVLHVCGGKGGNGVASVHREKFKPLGGPDGGNGGH
                     GGDVVLRVDPDVTTLVDYHHESHRRAGNGKQGMGDHRNGANGASLVLPVPDGTIVTTP
                     VGDVLADLIGAGTEAVIAAGGRGGLGNAALATKSRKAPGFALLGEAGEERTVILELKV
                     VADVGLVGFPSAGKSSLIAAISRARPRIADYPFTTLVPNLGVVTAGEMTYTVADVPGL
                     IEGASEGRGLGHDFLRHIERCAALLHVIDCATVEPGRDPVTDLDVLEAELEAHGGLDD
                     RPRLVALNKIDVPDARDLAELVRPQLEERGLKVFEISAATHEGLRELTYAMGELVSSA
                     RTVTTDVQPTRIVLRPGGTQADEFTISQVDGVWHVRGEKPQRWVAQTDFSNDEAVGYL
                     ADRLNRLGVEQRLLELGAEIGADVVIGDDDDAVVFEFDPQVQAGAEALHGRFGRRGQD
                     PRLDHR"
     gene            complement(6266..6523)
                     /gene="rpmA"
                     /locus_tag="VH419_06100"
     CDS             complement(6266..6523)
                     /gene="rpmA"
                     /locus_tag="VH419_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007492765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEY7043224.1"
                     /translation="MAHKKGAASTKNGRDSNAQRLGVKRYGGQLVNAGEIIVRQRGTH
                     FHPGTGVGRGGDDTLFALVEGNVEFGTRRGRRVVNVVPAAE"
     gene            complement(6553..6885)
                     /gene="rplU"
                     /locus_tag="VH419_06105"
     CDS             complement(6553..6885)
                     /gene="rplU"
                     /locus_tag="VH419_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020577386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEY7043225.1"
                     /translation="MYAIVRSGGQQQKVAVGDVIEIDKVATAPGESLSLPAVLVVDGD
                     TVTSDADSLAKVDVQAEVLGASKGPKIHILKYKNKTGYRKRQGHRQHYTKVKVTAINT
                     ETGKSSKK"
     gene            complement(7062..>9287)
                     /locus_tag="VH419_06110"
     CDS             complement(7062..>9287)
                     /locus_tag="VH419_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012919953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEY7043226.1"
                     /translation="PSAGPAKKTAKKGRSTASPGGDAPSEGGLAMPLFQAPEPVPAQT
                     RAPRTKDKEGAEPAEAVGATADPADQADSADQGDEAAPTRRRRSRGGRNRRKAAATEP
                     GADAVDAAADEPDSGGTPPAETASDGGDTATSHRRRRRRRSGEDTGGGQDDPAETTTR
                     VRAPRAKKESITSVEGSTRIEAKKQRRREGREAGRRRPPILSESEFLARRESVERVMA
                     IRQRGETTQIAVLEDNVLVEHYVALESQNSLIGNVYLGRVQNVLPSMEAAFIDIGKGR
                     NAVLYAGEVNWDLREDMNQPRRIENALKPGQSILVQVSKDPIGHKGARLTSQISLPGR
                     YLVYVPGGGTSGISRKLPENERNRLKNLLKDILPDDAGVIIRTAAEGASEDELTRDVT
                     RLTARWEDIDKKSRKGQAPALLYAEPDTTLKVVRDLFTEDFSKLVISGDDAWDMVDGY
                     IQHVAPDLVERLERYEGDGDVFENYRIDEQITKALDRKVWLPSGGSLVIDRTEAMTVV
                     DVNTGKFTGSGGNLEETVTKNNLEAAEEMVRQLRLRDIGGIIVIDFIDMVLETNRELV
                     LRRLVECLGRDRTRHQVAEVTSLGLVQMTRKRIGTGLLEAFSHVCEQCQGRGILVEGE
                     PADPKRHDDQHDHHGQGASRGSRRSSNRRRGKRGSDGHDHPDEASTDRQPEATDGATE
                     SAPQQPTADARAEPATPAVADAPSDGAGEGTPPPARGRRRRRASRPTGAPDVVGASPA
                     D"
BASE COUNT         1330 a         3284 c         3135 g         1538 t
ORIGIN      
        1 gtcagaccgt acgccggctg gacaactctg ggatggcgcg cgtcggggcc ggactcgacg
       61 cgcgcgacgc ctggcgtgca gccgtcgtac gacggcacgg catctcgttc ggcttcctgg
      121 ccttcaatgc gatcggagag actccccggg cgacggcgga aggcgcgggc gcggccgagg
      181 tcaggatgca gccccggacc ggaccgctcg acgccggcga ccttcgcagc gcgaccaccg
      241 ccgtgcgcac gcttgcccgt cgagtcgatg tggtcgtcgt ccttccgcac tggggagagc
      301 agtacacgaa ccggcctgtc cgtgaccagc ggaccgtcgg tgccgcgctg ctcgatgccg
      361 gtgccgacat ggtcgtgggt ggtcacccgc actgggtgca aggcatcgac gtgcatgacg
      421 gaaagctgat cgtcaactcg ctcggcaact tcgtcttcga catggacttc tccagccaga
      481 cccgccaagg cgtcctgctc gacgtggtct gctggaacga caagccggtg tcggccacgg
      541 tcactccgtt cctcatcggt gccgacttcg cgccccgtgt cgtccatggt ccggtggcgg
      601 cgacgatcct ggaccggatg tgggcgacga gcgacccacc cttccgtggc aatcgagttg
      661 gcgaaaccaa cggcaccgag gtggatcgtt gaaccgccgg acggctcagc gaccggcgag
      721 ccaggaccac atgaggacgc cgacgcgtcg aagctgcgcg ggattcacca cgcgaggcag
      781 gtcggcggcg ctgtggtagc cggcgtacga cgttccgcca acccgggcag caggcatccc
      841 ggcgagttcg aacgaccagt ggtcgctggc cgtgtcgacg catcggctcg tcaggaccgc
      901 ggcctgctca cctgctcgga ccagttgcgc ccggacatgc ggcggcagca gacctcccgc
      961 gcagagcggg accaccgttc cgacaccgac gcggtccagc gacaccatgc cgctgacggc
     1021 gcgccgctcc ggcttcgaca tccgagccac catgacccgc gacccgaagt ggtgcaaggc
     1081 ctcgccgtcg ccgcgtggct cttcggcggc gaaggcgacg aacatcgccg gcaaccgcgt
     1141 ctcgtgcgag gcggccatcc gcgccagctc gagcagtacc ccaacacccg acgcgtcgtc
     1201 ctccgcgcca ggcgcctgcg gcaccgtgtc gaggtgggcg ctcacgacca ggtgaggctc
     1261 tgcggcgtcg aagtcggtcg gtgtcgccac cacgttcgac gtcgtaccag ccgggactgg
     1321 cacaccccag gagatcccgg ccggcacagc gaggcgctgc cggcgtacgg cgtatccgta
     1381 ggcctcgaac cgtcccgcga cccagtcggc ggcccgttgg tatgccgtcg acgtcgcctc
     1441 gcgaggaccg aatcgcacgg ccaggacgcg cacggtgtcc attgcagccg acgaccggaa
     1501 caccggctcc tcgcgagccg gcgatgctgt ccgagcggtc ggtgacgatg aggcctgccc
     1561 gccgatcgag cttgtcgaga tcacgggctc agaccgccca gaaaggtgct cggcctcgtc
     1621 gttcccgggc gatccggtgc aggcaccgag cagcgcggtc gccgcgagga gcgccgccgt
     1681 ccccaaggag cgcgcttgca ccggactcag tcgcgggtct ggccggtgcc gatgaccacg
     1741 tacttcgtcg acgtcatctc cggcagcccc atcgggccgc gagcgtgcag cttctgcgtc
     1801 gagatcccga tctcagcgcc gaacccgaac tcgcccccgt cggtgaaccg ggtcgaggcg
     1861 ttcacgacca cggccgcggc atccactgca gcggcgaacc gacgcgcggc ctcctgcgag
     1921 cgggtgacga tcgcctcggt atggcccgtg ccgtaccggc ggatgtgctc gaccgccgcg
     1981 tcaccggagt cgacgacggc gacggacatg tcgagcgaga ggtactcggt gccgaagtcg
     2041 tcgtcggtgg cgggtacgac gtcggggctc aagcggcgta cgacctcgtc gccgtgcacc
     2101 atcacgcctt cggtcgcgag cgccttcacg atgcggggca cgaactcgtc ggccacggcg
     2161 gcgtgcacga gcagggactc ggcggcgttg cagacactcg ggcgctgggt cttggcgttg
     2221 acgacgatgc gttcggccat gtcgaggtcg gcatcggcat cgacgtacac gtgacagttg
     2281 ccgaccccgg tctcgatcac gggcacacgc gactcgcgga cgacccgatc gatgaggccg
     2341 gcacctccgc gcgggatcac cacgtcgacg agcccgcggg cgttcatcag gtggcccacg
     2401 tcgtcgcggt caccggtgac gagctggatc acgtcggcgg gcagaccagc cgatccgacc
     2461 gcgtcacgga gtacgtccac gaccgcggtg ttcgacctcg ccgccgagga cgagccccgc
     2521 agcaggacag cgttgccact cttcaggcag atcccggctg cgtcggcggt gacgttcgga
     2581 cggccttcgt agatcatcgc cacgacaccg aacggcaccc ggagctgccg catctccagc
     2641 ccgttggcca tcatcgagcc gcgcacgacc tcaccgaccg gatcgggcag gtttgccagc
     2701 tcccggagcc cctccgccat gccggtgacc cgctccgaag tcagccggag gcggtccacc
     2761 agtgccgccg ccgtgccggc cgactcctgc gccgacacat cctcggcgtt ggcgctcagg
     2821 atggcgtttt gcgcatgctc gagcgcgtca gccatggctc gaagtgccct gtccttggtg
     2881 gcccgcgtcg tcgtacccag gacgtacgac gcctcgcgtg cccgcctggc ggcggcgtgg
     2941 acgtcggctt tcgtcatgca gtcagcgtac ggcggcagct tcctgcgtcc ggacccgtct
     3001 cagccggcac caccgcccgt tcaacgaacc agaagtcacg gttcaacgaa ccacaaccca
     3061 cggctcaacg aaccacaacc cacggctcaa cgaaccacaa cccacggctc gacgaaccac
     3121 aactcacggt tcaacgagag aagcggcggg gggtggtgtc ggcctgggag agggggctgg
     3181 acaggggacg tgactggtag gtctcttgct cgaccacctc gagaccgacc accttccatg
     3241 gcggcagctc ggccgagccc cggtgctcgt tccacagccg cagcgccagc gaggcggcgt
     3301 cgaccatctc ctcgccctcc tcccagtacg cgatctcggc cttgtcttcg tcgtaccgga
     3361 tcgagtgcag gaagggctgt tgatgctgca gccgggacag cgcatcgtga acgttggaca
     3421 ccggctgcgg gtcaccggag acggtcacgg tcacgtgcca gagacgaacg gggaggctca
     3481 cacaggctcc tcgcgaaggg gatgggtgcc cgtcaagtct gcctgcacca ccgctgctct
     3541 gcacggtatt cggcgaagta ccgcccacga agagcaaccg gtgctacagg atcacgaggt
     3601 cgtcgcggtg caccagctct cgctcgtagc cttcgccgaa cgtcgccgcc agctccgacg
     3661 tcgtacgccc gaggaggctc gggatctccg cggcgtcata gttgaccagg ccgcggccga
     3721 tcgtttgtcc ggctgtgtcg accaggtcca cggggtcgcc ggcgccgaac cggccgtgga
     3781 cgccgacgac cccggccggc agcaacgagg tccctcgtgt cgtgacggcg tggacggcac
     3841 cgttgtccag caccagctgg ccgttcggct cggtggcgtg cgcgagccag agttgccggg
     3901 tcgaacgctt ccgacccgtc ggcgcgaacc aggtgcccga ctcaccggcc agtacggcac
     3961 gaatgtggtc ggcatgaccg acaacggtgg gaatgcccgc gccggtggcg atccgcgctg
     4021 cctccagctt ggtcgccatt ccaccgctgc cgagcgagcg ctccaccctt ctgccgacga
     4081 tcacgtgagc catctcgtcc ggaccgtgga cgacgtcgat gcgccgcgcc gacggatcgc
     4141 gcgggtcctt gtcgtagacc gcgtcgacgt cggacagcag gaccagcgcg tccgcgtgga
     4201 ccaggtgcga ggcgagcgcg gcaagccggt cgttgtcacc gaacctgatc tcgccggtgg
     4261 cgaccgtgtc gttctcgttc acgaccggca gcacgccgag ctcgagcagc ttcgcgaagg
     4321 ttcggtaggc gttgcggtaa tggctccggc gggtcatgtc gtcgacggtc agcaggacct
     4381 ggccgacttt caccccgtgc tcaccgaagg ccgaggtgta gcgatgcatg agcaggccct
     4441 gtcccacact ggctgctgct tgttgccggg ccaggtcgcg cgggcgggtc cgcaagccga
     4501 gcggcgcaag tccggccgcg atcgcccccg acgagaccag cacgatctcc agaccacctg
     4561 cccgcttggc agccagcgcg tccaccaggg tccgcagccg atggtcgtcg atcccgcctt
     4621 cggtcgtggt cagcgccgac gacccgactt tgaccacgac acgcttcgcg gcggccagtg
     4681 cctggacgcc gtacgccgcg ctcatcggtg gtcgagtcgc gggtcctggc cgcgccggcc
     4741 gaaccggccg tgcagcgcct cggctccggc ctgcacctgc gggtcgaact cgaagacgac
     4801 ggcatcgtcg tcatcgccga tgacgacgtc ggcaccgatc tcggcgccca gctcgagcag
     4861 tctctgctcc acaccgagcc ggttgagccg gtcggcgagg tagccgaccg cctcgtcgtt
     4921 gctgaagtcg gtctgcgcga cccaccgctg cggtttctcg cctcgcacgt gccagacacc
     4981 gtcgacctgg ctgatcgtga actcgtcagc ttgggttcct ccgggccgca gcacgatccg
     5041 ggtcggctgg acgtcggtcg tcacggtgcg agcggaactg accagctcgc ccatcgcgta
     5101 cgtcagctcg cgcaggcctt cgtgggttgc cgccgagatc tcgaagacct tcaagccgcg
     5161 ttcctcgagc tgcggccgta ccagctcggc gagatcccga gcgtccggga cgtcgatctt
     5221 gttcagcgcg accagtcggg gtctgtcgtc gaggccgcca tgcgcttcga gctctgcctc
     5281 cagcacgtcg aggtcggtga ccgggtcgcg gcccggctcg acggtcgcac agtcgatgac
     5341 gtggagcagc gccgcgcagc gttcgatgtg gcgcaggaag tcgtgcccga gcccacgccc
     5401 ttcgctcgcg ccctcgatga ggccaggcac gtcggcgacc gtgtaggtca tctctcccgc
     5461 agtcaccacg ccgagattcg ggacgagggt cgtgaacggg tagtccgcga tcctcggccg
     5521 tgccctggag atggcggcga tcaggctcga cttgccggca gacggaaagc cgaccaggcc
     5581 gacgtcggcg accactttga gctcgaggat gaccgtgcgc tcctcaccgg cttccccgag
     5641 cagggcgaag ccgggagcct tgcgcgactt ggtcgcgagg gcggcgttgc ccaggccgcc
     5701 gcggccgcct gccgcgatca ccgcctcggt cccggcgccg atcagatccg ccagcacgtc
     5761 gccgaccggg gtcgtgacga tcgtgccgtc gggcaccggc aggacgagtg aggcgccgtt
     5821 ggcaccgttg cggtggtcgc ccatcccctg tttcccgttg ccggcccgac gatgggactc
     5881 gtggtggtag tcgaccaacg tcgtgacgtc cggatcgacc cgcaggacga cgtcgccgcc
     5941 atgccctccg tttcccccgt ccggtccacc gagcggcttg aacttctccc ggtggaccga
     6001 cgccaccccg ttgccgccct tgccgccgca gacgtgcagg acgacacggt ccacgaatgt
     6061 cgggactgcc atgtcctgcc tcctccacct ctcaccgtcg cctgggggtc cccggaattg
     6121 cgggaaaagt cctgccgtga ggggggtctg gacctccact ttcccgcaat tcgcgggagc
     6181 ccggcaggac ggaaacatgc aacgaggggc ggccgcggct gcggcacacc cctcatgcgt
     6241 cgtgcaatgt cgtacgacgc gccggttact ccgcggccgg aacgacgttc acgacgcggc
     6301 gaccgcgccg ggtgccgaac tcgacgttgc cctcgaccag cgcgaacagg gtgtcgtcac
     6361 cgccacgccc gacaccggtg ccggggtgga agtgggtgcc ccgctgccgg acgatgatct
     6421 cgccggcgtt gacgagctgg ccgccgtagc gcttcacgcc gaggcgttgg gcgttgctgt
     6481 cgcggccgtt cttggtggac gccgcgccct tcttgtgtgc catgactgaa ctcctgtcga
     6541 acgccgcgtc ggctacttct tcgaagactt gccggtctcg gtgttgatgg ccgtgacctt
     6601 gaccttggtg tagtgctgcc ggtgcccctg gcgcttgcgg taccccgtct tgttcttgta
     6661 cttcaggatg tggatcttcg gacccttgct cgcgccgagc acctctgcct ggacgtcgac
     6721 cttcgcaagt gagtcggcgt cggacgtgac ggtgtcaccg tcgacgacca agacggcagg
     6781 cagcgacagg ctctcacccg gcgcggtcgc caccttgtcg atctcgatga cgtcgccgac
     6841 agcgaccttc tgctgctggc cgccactgcg cacgatcgcg tacacgcctc gactcactct
     6901 cttcggtagg ttctgaactc cgtcggcact gcgtgctgat gactgggagc ctcgccaaga
     6961 cgtcgacgcg tcccgcacga gtggtgcctc acaccgagcg tcaagtttac ggatccgacc
     7021 ccgcaccggt caaaacggtt gccgtccgga cgaggacagg gtcagtccgc gggagatgcg
     7081 ccgacgacgt caggggcccc ggtcggtcgg gatgcacgac gccggcggcg gccacgagcg
     7141 ggaggaggtg tcccctcacc cgccccgtcg gagggcgcgt cggcaacggc cggcgtcgcc
     7201 ggctccgcgc gagcgtcggc cgtcggctgt tgtggtgccg attccgtggc gccatcggtc
     7261 gcctccggtt gccggtcggt ggaggcctcg tcgggatggt cgtgcccatc gctgccgcgc
     7321 ttgccacgtc gacggttgct cgagcgccga gatcccctcg acgccccctg cccgtgatga
     7381 tcgtgctggt cgtcgtgccg cttcgggtct gcgggctcac cctcgacgag gatgccccga
     7441 ccctggcact gctcgcagac gtggctgaac gcctcgagca agccggtgcc gatgcgcttg
     7501 cgggtcatct ggacgagacc cagcgacgtg acctctgcca cctggtggcg cgtccggtcg
     7561 cggccgaggc actcgaccaa ccggcgcagc accagctcgc ggttggtctc cagcaccatg
     7621 tcgatgaagt cgatgacgat gatcccgccg atgtcgcgca gccggagctg gcggaccatc
     7681 tcctcagcgg cctccaggtt gttcttggtg acggtctcct cgaggttgcc acccgacccg
     7741 gtgaactttc cggtgttgac gtccaccacc gtcatcgcct cggtccggtc gatgaccagt
     7801 gaaccgccgg acggcagcca caccttccgg tcgagcgcct tggtgatctg ctcgtcgatc
     7861 cggtagttct cgaacacgtc cccgtcgccc tcgtagcgtt ccagacgctc aacgaggtcg
     7921 ggcgcgacgt gctggatgta cccgtcgacc atgtcccagg cgtcgtcgcc ggagatcacg
     7981 agcttggaga agtcctcggt gaacagatca cggacgactt tcagcgtggt gtccggctcg
     8041 gcgtacagca acgcgggagc ctggcccttc ctggacttct tgtcgatgtc ctcccagcgg
     8101 gctgtcagcc gcgtgacgtc gcgggtcagc tcgtcctcgc tggcgccctc ggccgcggtg
     8161 cggatgatga caccggcgtc gtcgggaagg atgtccttca gcaaattctt gagccggttg
     8221 cgctcgttct cgggcagttt tcggctgatg ccactggtgc cgccgccggg gacgtacacg
     8281 aggtagcggc cggggaggct gatctggctg gtcagccggg cccctttgtg gccgatcggg
     8341 tccttgctga cctggacgag gatggactgg ccgggcttga gggcgttctc gatccggcgg
     8401 ggctggttca tgtcctcacg aaggtcccag ttgacctctc cggcgtacag gacggcgttg
     8461 cggcccttgc cgatgtcgat gaacgccgcc tccatgctcg gcagcacgtt ctggacccgg
     8521 ccgaggtaga cgtttccgat cagcgagttc tgggactcca gcgcgacgta gtgctcgacg
     8581 agcacgttgt cctcgagtac ggcgatctgc gtggtctcgc cgcgctgccg gatcgccatg
     8641 acgcgctcga ctgactcacg ccgggccagg aactccgact cgctgaggat cggcgggcgt
     8701 cggcgaccgg cttcccgccc ttcccggcgg cgctgcttct tcgcctcgat acgcgtggat
     8761 ccctcgaccg aggtgatcga ctccttcttg gcccgtggcg cgcgcacccg tgtcgtggtc
     8821 tcagccgggt cgtcctgtcc tcccccggtg tcctctccgc tgcgccggcg ccggcgccgc
     8881 cgatgtgacg ttgctgtgtc accaccgtcc gaggcggttt cggctggagg tgtcccgccg
     8941 ctgtccggtt cgtcggccgc tgcgtctacg gcgtccgcgc ccggctcggt cgctgcggct
     9001 ttacgacggt tgcgtccgcc gcgcgagcgt cgacgccgtg tcggcgcggc ctcgtcgccc
     9061 tggtcggcgg agtcggcctg gtcggccggg tcggcggtgg ccccgacagc ttcggccggc
     9121 tcggcgccct ccttgtcctt ggtccgcggt gctcgcgtct gcgccggcac cggctccggg
     9181 gcctggaaca gcggcatggc cagcccgccc tcggacgggg cgtcgccgcc tggggaagcg
     9241 gttgacctgc cctttttcgc cgtctttttg gctggtcccg cggaagg
//
LOCUS       DATBFZ010000202         1598 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_6301895, whole genome shotgun sequence.
ACCESSION   DATBFZ010000202 DATBFZ010000000
VERSION     DATBFZ010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6301895"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1085)
                     /gene="kdpA"
                     /locus_tag="VH419_06115"
     CDS             complement(<1..1085)
                     /gene="kdpA"
                     /locus_tag="VH419_06115"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020661714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HEY7043227.1"
                     /translation="MSETTAGLLQTGLLLAALAVCYLPLGNYMARVFTSERDLAAERA
                     IYRMIGVDSKADQRWPVYARSVLAFSVVSVLVVYLLQRVQNHLPLSLGLPDVPPALSF
                     NTAVSFVTNTNWQAYSGEATMGHLVQMTGLAVQNFVSAAVGMAVAVALIRGFSRSQTD
                     RIGNFWVDLVRAVIRILLPIAVVGAFALVAMGAVQNFSGGVEATTLTGGQQTITGGPV
                     ASQEVIKELGTNGGGFYNANSAHPFENPNGLSNLFEIFLLLLIPVCLTRTFGVMVKDT
                     RQGYAILAAMAVLWVAIGAGVTVSEMTHHGSALQAAGGAMEGKEVRFGPFTSSVFATS
                     TTGTSTGAVDSFHSSFSALGGGLLILN"
     gene            complement(1085..1174)
                     /gene="kdpF"
                     /locus_tag="VH419_06120"
     CDS             complement(1085..1174)
                     /gene="kdpF"
                     /locus_tag="VH419_06120"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF021144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HEY7043228.1"
                     /translation="MSGSDAVGLVISVVLTGYLFIALLFPERL"
BASE COUNT          307 a          521 c          532 g          238 t
ORIGIN      
        1 atgttgagga tcaagagccc gccgcccagc gcgctgaacg acgagtggaa cgagtcgaca
       61 gcgccggtcg aggtccccgt ggtcgaggtg gcgaacaccg acgacgtgaa cggcccgaac
      121 cggacctcct tgccctccat cgccccgccc gctgcctgga gcgcgctgcc gtggtgggtc
      181 atctccgaga ccgtgacgcc ggcgccgatc gcgacccaca ggactgccat cgcagcgagg
      241 atcgcgtagc cctgtcgcgt gtccttgacc atgaccccga atgtccgtgt cagacagacc
      301 gggatcagga gcagcaggaa gatctcgaac aggttgctga ggccgttcgg gttctcgaag
      361 gggtgcgccg agttcgcgtt gtagaagccg ccaccgttgg tgccgagctc cttgatgacc
      421 tcctgcgacg cgaccgggcc gccggtgatt gtctgctgcc caccggtcaa cgtggtcgct
      481 tcgacgcctc cggagaagtt ctggaccgca cccatggcga cgagcgcgaa cgccccgacg
      541 actgcgatgg gaaggaggat ccggatgacg gctcgcacga ggtcgaccca gaagttgccg
      601 atccggtcgg tctgcgagcg ggagaacccg cggatcaagg cgaccgccac ggccataccg
      661 acggcggccg agacgaagtt ctgcacggcg aggccggtca tctggacgag gtgtcccatg
      721 gtggcctcac cggagtacgc ctgccagttc gtgttggtga cgaacgacac cgccgtgttg
      781 aaggagaggg ccggcggaac gtcagggaga ccgagcgaca gtggcagatg gttctggaca
      841 cgctggagca ggtagacgac gagcacgctg acgacggaga acgcaaggac cgatcgggcg
      901 tacaccggcc accgctggtc agccttcgag tcgaccccga tcatgcggta gatcgctcgc
      961 tcggcggcca ggtcccgctc gctcgtgaac acgcgcgcca tgtagttccc cagcggcaga
     1021 tagcagaccg ccagggcggc gagcagaagc ccggtctgca gcaggccggc ggtcgtctcc
     1081 gacatcagag tctctcgggg aagagcagtg cgatgaacag gtatccggtg aggaccacgg
     1141 agatgacgag gccgacggcg tcactgccgc tcacatccgc tccagtccgc gaagtaccag
     1201 cagtacgccg acgaagaacg cgaccgtcac ggcaacgaag atcaggtcct gcacgaggca
     1261 cgtcctttgc gatcgggaat gacgtcgacc agtcaaaccc gtcgatcgcg cgatgggacc
     1321 gtccctaacg gactgctaac gtgcaggccc cccagtcctg acgaaaccct cgcaacgcac
     1381 actcgaacgt tcaccgagca agcggtccgg ggtctcgaca ggctcgaccg ccggatcgcc
     1441 cggacccgcc tggcctgctg atcatcactg tcgagaccaa ggttcgtgtt gcggcccggg
     1501 gtctcgacaa gctcgaccgc cggatcgccc ggccgctgat cgagcttgtc gagatcaagt
     1561 ttcgtgttgc ggtccggggt ctcgacaagc tcgaccgc
//
LOCUS       DATBFZ010000203         3680 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_3084234, whole genome shotgun sequence.
ACCESSION   DATBFZ010000203 DATBFZ010000000
VERSION     DATBFZ010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 3680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3680
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3084234"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            261..2735
                     /gene="pepN"
                     /locus_tag="VH419_06125"
     CDS             261..2735
                     /gene="pepN"
                     /locus_tag="VH419_06125"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012921113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HEY7043229.1"
                     /translation="MRSLTAVEAAERAAAVAVTSYDIDLDLTRGSEQFGSHTTITFRS
                     LNGRPTFLDVRAAEVSSIVLNGSPIDVGLVDDGRLALDELATENTLVVDALMAYSHDG
                     EGLHRAVDPEDKQAYVYAMTFLPAAPRVFACFDQPDLKAIYRISVTAPTDWVVLGNGA
                     AEQIEPGRWTLAETKPLSTYFATLVAGPYHSITAEHDGIALGLHCRQTLAPHLDKDAE
                     ELFTVTGQCFDEYHRLFGIRYPFGEYHQAFVPEFNAGAMENPGCVTFTDELVFKAQAT
                     DSLRGTRAMVVAHEMAHQWFGDLVTMTWWDDLWLNESFAEYMGYRTTNDATKFADVWV
                     QNAFSRKAWGLAADQRSSSHPIAGNGAADAHEALTNFDGISYSKGASVLRQLNAYLGS
                     DAFVGGVVDYLHKHSYGNARLADLLESWDRASDKDVKAWAELWLRTQGVDTLSSEVVD
                     GSGSVTIRRENGSPEPVARPHAFTVTAYDTEGTGDQRSVVLDDDSVTVDFGRLDPDGL
                     VLPDSGGETWAKIVLDAGSLRRVPTLLPKLDDPIARAVVWGSLREGLLDAKVDPETYL
                     AAVESALPGDGDIAVESVLGGSRGGVIGALDLYFARPDDRDRMAAVVRTILDAAAAGS
                     NRQLIATRALIHATDDVAMLQGWLDGSAPDGLVTDEDFRWRVLRRLCALGVADHDDIS
                     REQDRDPSSQGALHALRCRASLPDPDVKAEVWQALTADRERSNYELYAMAECFFTPGQ
                     TDLTRPFVRRYFDDFPGIAEFRTGVLLEQITSMAYPRYAVDDETISLAEACLAGTDLD
                     TFVRRAVSDNTDDLRRVLRSTAAFPR"
BASE COUNT          600 a         1298 c         1213 g          569 t
ORIGIN      
        1 acacctgcga caccctccat gtcgcacgag acgaacacct tcacccgcgt cctcctcggc
       61 cggcttggac tgctgccggc tcgtcagtcg atcctgccac gtggcggcgc ccggataccc
      121 gccacggtgg tgtgccgccg cccggatacc cgccacggtg gtgtgccgcc gcctgcgtcg
      181 gtatcgggtt ggcaacgatg cctgcccggt acgtcggcgc ggctgcgcgc gttggctcgt
      241 cgcgtcctag ggtggtccgc atgcgcagtc tcaccgctgt tgaagccgcc gagcgtgccg
      301 ccgccgtcgc tgtcacgtcg tacgacatcg acctcgacct gacccgcggt tcggagcagt
      361 tcgggtcgca cacgacgatc accttccgca gcctcaacgg ccggccgacg ttccttgacg
      421 tccgggcggc agaggtctcc tcgatcgtgc tcaacggatc gccgatcgac gtgggcctgg
      481 tggacgacgg tcggctcgca ctggacgagc tggcaaccga gaacacgctg gtcgtcgacg
      541 cactgatggc ctactcgcac gacggcgagg gtctgcaccg cgccgtcgac cccgaggaca
      601 agcaggcgta cgtgtacgcg atgacgttcc tcccggcggc cccgcgtgtg ttcgcctgtt
      661 tcgaccagcc cgatctcaag gcgatctacc gcatctcggt gactgcgccg accgactggg
      721 tcgtgctcgg gaacggggca gccgagcaga tcgagcccgg ccggtggacg cttgccgaga
      781 ccaagccgct gtcgacgtac ttcgccacac tggtcgccgg gccctaccac tccatcactg
      841 ccgagcacga cgggatcgcg ctcggcctgc actgccgaca gacgctcgcg ccgcacctgg
      901 acaaggacgc cgaggagctc ttcacggtca ccggtcaatg cttcgacgag taccaccggc
      961 tgttcgggat ccgctatccg ttcggtgagt accaccaagc cttcgtgccg gagttcaacg
     1021 ccggcgcgat ggagaaccct ggctgcgtga cgttcactga cgagctggtc ttcaaggcac
     1081 aggcgaccga ctccctccgt ggtacccgtg cgatggtcgt cgcccacgag atggctcacc
     1141 agtggttcgg cgatctcgtc acgatgacgt ggtgggacga cctctggctg aacgagtcct
     1201 tcgcggagta catgggttac cgcaccacca atgacgcgac gaagttcgcc gatgtctggg
     1261 tccagaacgc gttcagccgg aaggcctggg gtctggctgc cgaccagcgg tcctcgagcc
     1321 acccgatcgc cggcaacggc gccgccgacg cgcacgaagc gctgaccaac ttcgacggga
     1381 tctcctacag caagggcgcg tccgtactcc gccagctcaa cgcgtacctg ggcagcgacg
     1441 cgttcgtcgg cggcgtcgtc gactacctgc acaagcactc gtacgggaac gctcggttgg
     1501 cggacctgct ggagtcgtgg gaccgggcga gcgacaagga cgtgaaggcg tgggcggagc
     1561 tgtggctgcg cacgcagggg gtcgacacgc tgtcgtcgga ggtcgtcgac ggctccgggt
     1621 cggtgacgat tcgccgcgag aacggatcac ccgaaccggt cgcgcgccca cacgcattca
     1681 ccgtgacggc gtacgacacc gagggcactg gcgaccaacg ctcggtcgtg ctcgacgacg
     1741 actcggtcac ggtcgacttc ggccggctcg atcctgacgg cctcgtgctg ccggactccg
     1801 gtggcgagac ctgggcgaag atcgtgctgg acgcgggctc gttgcggcgc gttccgaccc
     1861 tgttgccgaa gctcgacgac ccgatcgccc gcgccgtcgt ctggggatcg ctgcgcgagg
     1921 ggctgctcga cgccaaggtc gatcccgaga cctacctcgc cgcggtcgag tccgcactcc
     1981 cgggcgacgg ggacatcgcc gtcgagagcg tgctcggcgg ttcccgcggc ggggtgatcg
     2041 gcgccctcga cttgtacttc gcccggcccg acgatcgtga ccgaatggcg gccgtggtcc
     2101 ggaccatcct cgacgcggcg gcagccggga gcaaccgaca gctgatcgcc acgcgagctc
     2161 tcattcacgc caccgacgac gtggcgatgc tgcaggggtg gttggacggt tcggccccgg
     2221 atggcctggt cacggacgag gacttccgct ggcgtgtctt gcgtcgcctg tgcgcgctcg
     2281 gtgtcgcgga ccacgatgac atcagccggg agcaggatcg tgacccgtcc tcgcaaggag
     2341 ccctgcatgc cttgcgctgt cgtgcgtcac tgccggatcc ggacgtcaag gcagaggtct
     2401 ggcaggcgct cacggccgac cgtgagcgga gcaactacga gctgtacgcc atggccgagt
     2461 gcttcttcac gccgggccag accgacctga cccggccgtt cgtgcgacgc tacttcgacg
     2521 acttccccgg catcgcggag ttccgaaccg gagtcctgct cgagcagatc acctccatgg
     2581 cctatccgcg gtacgccgtc gacgacgaga cgatctccct ggccgaggcc tgccttgccg
     2641 gcaccgacct cgacacgttc gtccgccggg cagtcagcga caacaccgac gacctgcgcc
     2701 gggtccttcg cagcacggcc gccttccccc gctgatccac gcgccgccgc cgcgcccgcc
     2761 gccgctgcac cgccatcgga cagtggcgct tggaggtttg cgatgtttac agcccaaaag
     2821 gcgtgtaaac atcgcaaacc tcagatgggc accgtgggtt tgggatgtga acatctcaag
     2881 ccgcggtcgg gaccgcagcg accaacgccc ggaaagcccc cttcgtcacc tatcggacac
     2941 gtgccggtgg gggtttgcct gggtagccat ccgaatgcac gggtagccag gcagatctgc
     3001 cttgcgagcg gtgggtttgc atggcgcccc cgagggaaac cgcgacccgg tcagcggcgg
     3061 atgtctgaag cgcggccggt catacaccag gcgaaggcaa gggaaagccg gccgacctgc
     3121 tcaacccgcg cggccgtttc ccgctcttca ctgccaaccc ggcccagccg acgcggaggg
     3181 agcgtctggc gacaacgccc gcctaccgag tcccttcccg ctcacagaac ccgaggagaa
     3241 cccggggctg ccatgcgacc ggagggttgc atggtgcctg aggcaaaccg tgacccggtc
     3301 gacagacggc ggatgtccaa accggtggtc acaggccagc aagggaaagc cggccgacca
     3361 actcaacggg ccgcggacgc ttcccgctca tcgctgcgaa cccgccccag ccgaggcgag
     3421 ggagcatctg gggaccaggc ccacccaccg agtccccttc ccgctcacag aacccgagga
     3481 gaacccgaag cggccttgcg accggtgggt ttgcatgctg cgggaggcag accgcgaccc
     3541 ggtcatccgc ggatgccgaa tctcctccgg tcacacacag caggcgcagg caagggaagg
     3601 ccggccgatc acctccgggc gcggaggtct tccgctcttc actgccgacc cgccccagcc
     3661 atgacgacgg agcgcctggc
//
LOCUS       DATBFZ010000204         4653 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_3084663, whole genome shotgun sequence.
ACCESSION   DATBFZ010000204 DATBFZ010000000
VERSION     DATBFZ010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 4653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4653
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3084663"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..329)
                     /locus_tag="VH419_06130"
                     /pseudo
     CDS             complement(1..329)
                     /locus_tag="VH419_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009078048.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
     gene            306..701
                     /locus_tag="VH419_06135"
                     /pseudo
     CDS             306..701
                     /locus_tag="VH419_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011753623.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
     gene            920..1861
                     /locus_tag="VH419_06140"
     CDS             920..1861
                     /locus_tag="VH419_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017933615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043230.1"
                     /translation="MNTADLFDTPFTLTMASQAGLTPDRLRRLVEDGAVRRVVRGVYV
                     TNSIPDTVLLRAQAAALVISPATVLCDRTAAWLHGIDVFRWAERDVLPTLETFVLRYH
                     ARHSREGLYGGQRDLLPSDVMVIHGVQVTTPLRTALDLGCALPRRDALAALDSFMRVF
                     GLTQHQLRLEATRYFRRRGVRQLRGLLPLADPRAESPGESWTRLEIIDSRFPAPQLQW
                     SVRRNGGELFRLDLAYPGLKIAIEYDGEEFHDSAESHLADEQRRRWLEEHGWIVIVVR
                     KEDFTREAIFQWTEQLRRAIDQRRVTVRLRPGTHNPA"
     gene            complement(1984..3423)
                     /locus_tag="VH419_06145"
     CDS             complement(1984..3423)
                     /locus_tag="VH419_06145"
                     /inference="COORDINATES: protein motif:HMM:NF018012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HEY7043231.1"
                     /translation="MFRNTMPRYEVLSEDAMATLDKGWRRIVTEIGVEFASERAVELF
                     RKAGQRVEDNSVFLDPDFVLEQVAKAPREFDVAARNPANSIHVGGDSMAFGAVYGPPF
                     LREGDVRRDATMEDFRNFAKLAQSYAVMDSAGGVVCEPNDTPLDSRHLDMTYALQTLT
                     DKVYMGNVVSGVNAADTIAMGSILFGGRDVIEQAPVSISLINCNSPLRWDDRMLDAQF
                     EYCEAAQPVVLTPFLLMGAMSPVTIPATLVQQMAEALSGIALSQLIRPGCPVIFGSFL
                     SNIDMQSGSPCFGTPESAIGLFCTGQIARHFGLPFRSGGGLTSSQVPDAQAGYEAMMT
                     MLPTFLAGTNWVMHSAGWLEGGLVAGYEKFIVDVQLLEMLQVEFTPLEVDEASLAFDA
                     HQEVGHGGHFLGAMHTMERFRTCFYRPFLSSSDNYERWVRNGGLDTAARATKIYQDKL
                     EAYEPPPLDDAIRAELDDFVSRRRTELGD"
     gene            3579..4418
                     /locus_tag="VH419_06150"
     CDS             3579..4418
                     /locus_tag="VH419_06150"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEY7043232.1"
                     /translation="MTGNGTQAIDRAAELLSLVVLADGRPGFGDLVQDTGLAKSTASR
                     LLQALERHRLVHRDDRGGYVPGALFALYASRHEPLDELISLARPTMQQLNDATGETVN
                     LAVARNNTVVQVAQIDSTFLLGTVNWVDVDVPAHCSALGKLFYAAGAIGLPTSQLERR
                     TEHTITDPAQLARQLEQIRSDGYAVTLGELEIGLDAIAVPVHGQLQGRPDQIVAAIGI
                     SGPSDRIHHRLQQLSELLKRQAAELSGALGHPGRVAGDHTPNQSDDTRTLPSTPTGKE
                     GAA"
     gene            4415..>4653
                     /locus_tag="VH419_06155"
     CDS             4415..>4653
                     /locus_tag="VH419_06155"
                     /inference="COORDINATES: protein motif:HMM:NF014646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing protein"
                     /protein_id="HEY7043233.1"
                     /translation="MTPDEILQALYDETLVGNAPAVLDLTRQGLDQGMAPETMLFDAL
                     IPSLEEVGARFERGDFFVPEMLIAGRAMSGALEILR"
BASE COUNT          802 a         1594 c         1489 g          768 t
ORIGIN      
        1 cagcggatgg cgcgggcgag cacctgggcc tcgacgtcgc gccccgcggc ggtcatgtcc
       61 tcggggtcga gcgagtggtc gacgcggacg acgtcctgct cgatgatcgg gccctcgtcg
      121 aggtcggggg tgacgtagtg cgccgtggcg ccgaccagct tcacgccgcg cgcgtgcgcc
      181 tggtggtagg gcttggcgcc cttgaagctc ggcaggaacg agtggtggat gttgatcgcg
      241 cggccggaca ggtcggcgca caagccgtcc gacaggacct gcatgtagcg cgcgagcacg
      301 accaggtgga tgccgtgctt gtcgacgagc tcggcatcca tctggtggtg ttggcgcggt
      361 acatgcaggt gttgtcggac gagacctgcc gggtgctgag tgggcgcgcc atcaacatcc
      421 accactcgtt cctgccgagc ttcaagggcg cgcggccgta ccaccaggcg caccaacggg
      481 gcgtgaagct gatcggcgcg accgctcact acgtcacatc ggacctcgat gaggggccga
      541 tcatcgagca ggacgtcgct cgcgtggacc acaactacac ggccgagcag ctggtggccg
      601 cggggcgcga cgtggagagc tcagtccttt cccgcgccgt tcgttggcac tcggagtccc
      661 gcgtcatgct caacggctcc aaaaccgtcg tcttccgcta acccccgccc ccgttgagtg
      721 ctgaatttct ggtcgttgag tgcggggttc ttgggcgtcg agtgcgggat ttctggacat
      781 tgaatgcagg tttcaaaggt gtcgagtgct gggttgcctg ctggaaccgg gaccgcccca
      841 tgtgaaagca ggaccgacgg tcctcgcctg tggatgatcg acgcgccgcg gccgacatct
      901 gaggcatcgt cggttcgcca tgaatacagc ggacttgttc gacactccct tcaccctcac
      961 gatggcttcg caagcaggac tgacgcccga tcgactacgt cggctggtcg aagacggcgc
     1021 ggttcgtcgc gtggttcgcg gtgtctatgt cacgaactcc attcccgaca ccgttcttct
     1081 gcgggctcag gccgccgcac tcgtcatctc gcctgcaacg gtgctgtgtg accgcacagc
     1141 tgcctggctc cacggcatcg acgtcttccg ttgggcggag cgtgatgtgc tcccgacgtt
     1201 ggagaccttc gtcctccgct atcacgccag acattctcgc gaggggctgt acgggggcca
     1261 acgagacctg ctgccttctg acgtcatggt gatccacggt gtgcaagtca cgacaccgct
     1321 gcgcacggcg ctcgaccttg gctgtgcctt gccgaggcgt gatgccttgg cagcacttga
     1381 ttcgttcatg cgcgtgtttg gcttgacgca acaccagctg cgcttggagg cgactcgcta
     1441 ctttcgtcgg cgaggcgttc ggcaactccg aggtctcctt ccattggctg acccccgagc
     1501 tgagtcgccg ggcgagtcct ggacccggct cgagatcatc gactcgcgct ttcccgcacc
     1561 gcagttgcag tggtccgtga gacgcaacgg cggagaactg ttccggctcg atctggcgta
     1621 ccccggactc aagatcgcga tcgagtacga cggtgaggag ttccatgact cagccgagag
     1681 tcacctcgcc gacgagcaac ggcgtcgctg gctcgaagaa cacggatgga tcgtcatcgt
     1741 ggttcgtaaa gaagacttca cgcgggaagc aatcttccag tggaccgaac agcttcgacg
     1801 tgccatcgat cagcggcgcg tcaccgtgcg gctgcggccc ggcacgcaca atcccgcctg
     1861 agtggttcac atgatggcca gcactcaacg agcaagagct cgggcactca acgccccgga
     1921 agccagcact caacgccccg gaagccagca ctcaacggcc cggaagccag cactcaacgc
     1981 aggtcagtcg ccgagctcgg tgcggcggcg ggagacgaag tcgtcgagct cagcacggat
     2041 ggcgtcgtcc aagggaggcg gttcgtacgc ctcgagcttg tcctggtaga tcttcgtggc
     2101 ccgggcagca gtgtcgagtc cgccgttcct gacccacctc tcgtagttgt ccgacgagga
     2161 caggaacggc cggtagaagc aggttcggaa ccgctccatc gtgtgcatcg cccccaggaa
     2221 gtgaccgcca tgcccgacct cctggtgcgc gtcgaacgcc aacgacgcct cgtcgacctc
     2281 cagcggcgtg aactcgacct gcagcatctc caacagctgt acgtcgacga tgaacttctc
     2341 gtaccccgcc accaggccgc cttcgagcca cccggccgag tgcatcaccc agttcgtgcc
     2401 ggccaggaac gtcggcagca tcgtcatcat cgcctcgtac cccgcctgcg catcgggcac
     2461 ctggctggac gtcaaccccc cgccggaccg gaacggcagg ccgaagtgcc gggcgatctg
     2521 tccggtgcag aacagaccga tcgccgactc cggagtaccg aagcacggcg agcccgactg
     2581 catgtcgatg ttggacagga acgagccgaa gatcaccgga caacccggcc ggatcagctg
     2641 cgacagcgcg atccccgaca gcgcctccgc catctgctgc accaacgtcg ccggaatcgt
     2701 caccggtgac atggcgccca tgagcaggaa cggcgtcaac acgacaggct gagccgcctc
     2761 gcagtactcg aactgcgcat cgagcatccg gtcgtcccac cgcagcggcg agttgcagtt
     2821 gatcagcgag atcgacacag gcgcctgctc gatgacgtcc cgaccgccga acaggatcga
     2881 ccccatcgcg atggtgtcgg cagcgttcac gcccgacacc acgttgccca tgtagacctt
     2941 gtcggtcagc gtctgcagtg cgtacgtcat gtcgaggtgg cgcgagtcca gcggcgtgtc
     3001 gttcggctcg cacaccaccc cgcccgcgga gtccatcacg gcgtatgact gggcaagctt
     3061 ggcgaagttc cggaagtcct ccatggtcgc gtcgcggcgt acgtcgccct cgcgcaggaa
     3121 cggcggaccg tagacggcgc cgaacgccat cgagtcgccg ccgacgtgga tggagttcgc
     3181 cgggttgcgc gcagccacgt cgaactccct gggcgccttc gccacctgct cgaggacgaa
     3241 gtcgggatcg aggaagacgg agttgtcctc gacgcgctga cccgccttcc ggaacagttc
     3301 caccgcgcgc tccgaggcga actccacccc gatctcggtc acgatccggc gccatccctt
     3361 gtcgagcgtg gccatcgcgt cctcggagag gacctcgtag cgcggcatcg tgttgcggaa
     3421 catgcggcct cccaaccgtc cacgccatcg accgcacctg ttccgagaac cgaacgctgc
     3481 gtcatgatgt ggaacttcgg tttcatattg tggtttcatt gtggtggaag aacccaccat
     3541 gcggaagacc cgccccccaa ccttcgagga cccgacccgt gaccggcaac ggaacccagg
     3601 cgatcgacag agcagccgag ctcttgtcgc tggtggtgct cgccgacggg cgaccgggct
     3661 tcggggatct cgtgcaggac accgggctgg ccaagtcgac ggcgtcgcgg ctgctccagg
     3721 cgctcgagcg gcaccggctg gtccaccgcg acgaccgcgg cggctacgtt cccggcgcgc
     3781 tgttcgccct ctacgccagc cggcacgagc cactggacga gctcatcagc cttgcccggc
     3841 cgaccatgca acagctcaac gacgcgaccg gtgagaccgt caacctcgcc gtcgcccgca
     3901 acaacaccgt cgtacaggtc gcccagatcg actcgacgtt cctgctcggc accgtgaact
     3961 gggtcgacgt cgacgtgccg gcacactgtt cggcgctcgg caagctgttc tacgccgccg
     4021 gcgccatcgg gctccccacc tcgcagctcg agcggcgtac cgagcacacg atcaccgacc
     4081 ccgcccagct ggcccgtcag ctcgagcaga tccgctccga cgggtacgcc gtgacactcg
     4141 gtgagctcga gatcggcctg gacgcgatcg ccgtgcccgt ccacggtcag ctgcagggcc
     4201 ggccggacca gatcgttgcc gcgatcggca tctccgggcc gagcgaccgc atccaccacc
     4261 ggctgcagca gctcagcgag ctgctcaagc ggcaggcggc cgagctgtcg ggcgcgctcg
     4321 ggcaccccgg ccgcgtggcg ggcgaccaca caccgaacca gagcgacgac acgcggacgc
     4381 tcccatcgac tccgaccggg aaggagggcg ccgcatgacg cccgatgaga ttctgcaagc
     4441 gctgtacgac gagaccctgg tcggcaacgc cccagcggtc ctcgatctga cccggcaagg
     4501 tctcgaccag ggcatggcgc cggagacgat gctgttcgac gcgctgatcc cgtccctgga
     4561 agaggtcggc gccaggttcg agcgcggcga cttcttcgtg cccgagatgc tgatcgccgg
     4621 ccgggcaatg agcggcgcac tcgaaatcct gcg
//
LOCUS       DATBFZ010000205         3829 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_1120009, whole genome shotgun sequence.
ACCESSION   DATBFZ010000205 DATBFZ010000000
VERSION     DATBFZ010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 3829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3829
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1120009"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="VH419_06160"
     CDS             complement(<1..534)
                     /locus_tag="VH419_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008355548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HEY7043234.1"
                     /translation="MADARAPRAPDPGRDHPSAPPVPHDPAGLETARALASGLRGETR
                     RRPRSANAASSSRPAGQLLSGAHPDARDPAPVGSAVERLVTENGWESELAVHGVFGRW
                     DTIVGTEVAAHCGPESFADGRLTVRTDSTAWATQLKLLAPTVVRRLNEELGDGTVVRI
                     EVVGPASPSWRRGPRTVR"
     gene            complement(527..1672)
                     /gene="recF"
                     /locus_tag="VH419_06165"
     CDS             complement(527..1672)
                     /gene="recF"
                     /locus_tag="VH419_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008355545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEY7043235.1"
                     /translation="MHVSHLSLIDFRSYAEAEIELSSGVSTFLGPNGQGKTNLVEAIE
                     YVATQASHRVASDQPLVRRGADRAIVRSTVHRDDRQALIELEINPGKANRARLNRGAV
                     SRVRDVVGILRAVVFSPADLELVKGDPGERRRFLDDLLVARQPRYAGVRADYERVLKQ
                     RNTLLKTARGRAGRQRSDAALSTLDVWDAHLARTGAELLEARLRLVDDLRPHVTRHYA
                     DVALAVPKPDGGNDATLEYRPSLAIEAGADDRAGLAEALLAEVVKRRDDELDRGITLV
                     GPHRDDLLVSLGPLPARGYASHGESWSLALGLRLGAFELLRQDGDDPVLILDDVFAEL
                     DDSRRGRLAELVLVSEQVLVTAAVRDDVPAKLMGQPFEVREGAVTRG"
     gene            complement(1753..2670)
                     /gene="gnd"
                     /locus_tag="VH419_06170"
     CDS             complement(1753..2670)
                     /gene="gnd"
                     /locus_tag="VH419_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015069991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HEY7043236.1"
                     /translation="MQIGLIGLGKMGGNMRERLRRGGHTVVGYDRDAELSDVASLEEL
                     VAALEAPKVVWVMVPAGNPTRDTVQRLGGLLGEGDVVVDGGNSRWTDDTAHAEILSTK
                     GIGFVDCGVSGGVWGLENGYALMSGGAKEHVAKVQPAFDTLKPDGDFGYVHAGPVGAG
                     HFTKMVHNGIEYGIMQAYAEGYELLEKAEVCENVTDVLRSWREGTVIRSWLLDLLVAA
                     LDDDADLSHIAGWAEDSGEGRWTIEAAIDHAVPMHSITAALYTRFQSRQDESPAMKAV
                     AAMRNQFGGHAVRAGGGKGATPSADVDTA"
     gene            complement(2707..>3829)
                     /gene="dnaN"
                     /locus_tag="VH419_06175"
     CDS             complement(2707..>3829)
                     /gene="dnaN"
                     /locus_tag="VH419_06175"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020574616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEY7043237.1"
                     /translation="DVLAEAVAWTARSIPTRPGTLPQLSGILVRTGQDGLTLSGFDYE
                     TSGQVTIPADVADDGEVLVSGRLLADIARALPDRPVDVALDGPKLAVTCGSSRFSLQT
                     MPVEDYPALPAMPAAAGTVDADRFATAVSQAVTAAGRDDMLPVLTGVKLEIDGSQLSL
                     LATDRFRLSLRELQWDPHQPDISVNALVPARVLADTARSLASGTQLTIALSAAGAEGL
                     IGFESEAAGGRRRTTTRLIEGAFPNVRQLFPSASDITAQVDVATLVDSVKRVSLVAGR
                     NTPIRLGFSEGVVTLEAGNGEEAQASESLEAVLEGEPITIGFNPSYLLDGLQAIDGSH
                     VHIAMTQPARPAAFASMAGADAEPDLAFRYLLMPLRMQG"
BASE COUNT          560 a         1363 c         1314 g          592 t
ORIGIN      
        1 acggactgtg cgcggccctc gtcgccacga cggcgatgcc ggcccgacga cctcgatccg
       61 tacgacggtc ccgtcaccga gctcctcgtt cagccggcgt acgacggtcg gtgccagcag
      121 cttcagctgg gtcgcccagg ccgtggagtc ggtccggacg gtgagccgtc cgtcggcgaa
      181 gctttccggt ccacagtgcg ccgccacctc ggtcccgacg atcgtgtccc acctgccgaa
      241 cacgccgtgc accgcgagct cgctctccca tccgttctcg gtgacgagcc gttcgaccgc
      301 ggagccgacc ggggcagggt cgcgcgcgtc cggatgtgca cccgacagca gctgtccggc
      361 gggacgggac gacgatgcgg cgttcgccga gcgcggacgg cgtctcgtct cgccgcgcag
      421 cccggacgcg agcgctcgcg ctgtctcgag tccggcgggg tcgtgcggga ctggcggcgc
      481 cgacgggtgg tcacgcccgg gatccggcgc gcgtggcgcg cgcgcgtcag ccacgagtga
      541 ccgccccttc cctcacctcg aatggttgac ccatcagctt cgccggaacg tcgtctcgga
      601 ccgccgccgt caccaagacc tgctcgctca ccagcaccag ctccgccagc cgaccacgcc
      661 tggagtcgtc gagctccgcg aagacgtcgt cgaggatgag caccgggtcg tcaccgtctt
      721 gccgcagcag ctcgaaggcc ccgagccgaa ggcccaacgc caacgaccat gactcaccgt
      781 ggctcgcgta tcccctggcg ggcaacggac ccagcgagac gagcaggtcg tcgcgatgcg
      841 gcccgacgag cgtgatgccg cggtcgagct cgtcatcccg gcgtttcacc acctcagcca
      901 gcagcgcttc cgccaagccg gcgcggtcgt cggcgccggc ctcgatcgcg agggaaggcc
      961 ggtactccaa cgtggcgtcg ttcccgccgt ccggtttcgg aaccgctagg gcaacgtcgg
     1021 catagtgccg tgtcacgtgg ggccggaggt cgtcgaccag ccggagccga gcctcgagaa
     1081 gctctgcgcc ggtccgggcc aggtgtgcgt cccagacgtc cagcgtcgac agggccgcat
     1141 cgctacgctg ccggccggcg cggccccgtg ccgtcttcaa caacgtgttg cgctgcttga
     1201 gcacgcgttc gtagtcggct cgtacgccgg cgtaccgtgg ctgacgcgcc accagcaggt
     1261 cgtcgaggaa ccgtctgcgc tcgcccgggt cacccttgac cagctcgagg tcggcgggtg
     1321 agaagaccac ggcccggagg atcccgacca cgtcacggac gcgggagaca gcgcctcggt
     1381 tgagccgagc ccggttcgcc tttccggggt tgatctcgag ctcgatcagg gcctgccggt
     1441 cgtcacggtg cacggtggac cgcacgatgg cgcgatcggc gccacgtcgg accagcggct
     1501 ggtcgctggc cacccggtgc gacgcctgcg tcgcgacgta ctcgatggcc tcgacgaggt
     1561 tggtcttgcc ctgcccgttg ggcccaagga aggtcgacac gccggaggac agctcgatct
     1621 cggcctcggc gtacgaccgg aagtcgatga gcgagaggtg ggagacgtgc atgatgtgcg
     1681 ccgactacgc ggaccgggtc gcctcgcggg tgcccatcag cagagtcatc aggatcagca
     1741 cagtcagcac agtcatgccg tgtcgacgtc cgccgagggt gtcgcgccct tgccaccgcc
     1801 ggcgcgcacc gcatgtccgc cgaactggtt gcgcatcgcg gccaccgcct tcatcgccgg
     1861 tgactcgtcc tgccgtgact ggaagcgggt gtagagcgcc gcggtgatgg agtgcatggg
     1921 aaccgcgtgg tcgatcgcgg cttcgatcgt ccatcgaccc tcgccggagt cctcggccca
     1981 gccggcgatg tgggacaggt cggcatcgtc gtcgagtgcc gcgaccagca ggtcgagcag
     2041 ccacgagcgg atcaccgttc cttcgcgcca ggagcgcagg acgtcggtga cgttctcgca
     2101 gacctcggcc ttctcgagca gctcgtaccc ctcggcgtag gcctgcatga tgccgtactc
     2161 gatgccgttg tggaccatct tcgtgaagtg gccggcgccg accggcccgg cgtgcacata
     2221 gccgaagtcg ccgtccggct tcagtgtgtc gaacgccggt tgcaccttgg cgacgtgttc
     2281 cttggccccg ccggacatga gcgcgtatcc gttctccagc ccccagacac cacctgaaac
     2341 cccgcagtcg acgaacccga tgcctttggt cgagaggatc tctgcgtgcg cggtgtcgtc
     2401 ggtccagcgg gagttgccgc cgtcgacgac cacgtctccc tcgccgagca gcccgccaag
     2461 tcgttgcacc gtgtcgcggg tcgggttgcc ggcgggcacc atcacccaca cgaccttcgg
     2521 ggcctcgagc gccgctacga gctcctcgag gcttgccacg tcgctgagct cggcgtcgcg
     2581 gtcgtagccg accacggtgt gacctccccg gcgcagccgt tcgcgcatgt tgccgcccat
     2641 cttgcccagg ccgatgagtc cgatctgcat gcggtgatcc tttcgcgcgg agccttccgg
     2701 acgcactcag ccttgcatgc gaagcggcat gagcaagtag cggaaggcaa ggtcgggttc
     2761 ggcgtccgca cctgccatgc tggcgaacgc cgccggccgc gccggctgtg tcatcgcgat
     2821 gtggacatgg ctaccgtcga tggcctgcag cccgtcgagc aggtacgacg ggttgaagcc
     2881 gatcgtgatc ggctcgcctt cgagaactgc ctccagcgac tccgacgcct gggcctcctc
     2941 gccgttgccg gcttcgagcg tcaccacgcc ttcggagaag ccgagccgga tcggcgtgtt
     3001 ccgcccggcc accaacgaga cacgcttgac cgagtccacc agcgtggcga catcgacctg
     3061 ggccgtgatg tccgacgcgc tcgggaacag ctgccggacg ttggggaacg cgccctcgat
     3121 gagcctcgtg gtcgtccgcc gtcgtccacc cgctgcctcc gactcgaacc cgatgagacc
     3181 ctcggcgccg gcggcggaca acgcgatcgt gagctgagtc ccggacgcga gcgagcgggc
     3241 cgtgtccgcc agcacccgcg ccgggaccag tgcgttgacg ctgatgtccg gctggtgcgg
     3301 gtcccactgg agctcccgca ggctgagacg gaaacggtcg gtcgccagga gcgacagctg
     3361 cgacccgtcg atctccagct tgacgcccgt gagcaccggc aacatgtcgt cccggccggc
     3421 cgctgtgacc gcctggctga cggcggtggc gaagcggtcg gcgtcaacgg tgcccgcggc
     3481 cgccggcatc gccggaagcg ccgggtagtc ctcgaccggc atggtctgga ggctgaagcg
     3541 cgagctccca caggtgaccg ccaatttggg gccgtccaac gccacgtcca ccggccggtc
     3601 aggtagtgcc cgggcgatgt cggccagcag ccggccggac acgagcactt cgccgtcgtc
     3661 cgcgacgtcc gcagggatcg tgacctggcc agacgtctcg tagtcgaagc cgctcagggt
     3721 caacccgtcc tggccagtcc ggacgaggat tcccgaaagc tgtggcaggg tgcccggccg
     3781 ggtggggatg gagcgggcgg tccaggcgac ggcctcggcg aggacgtca
//
LOCUS       DATBFZ010000206         7615 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_2757767, whole genome shotgun sequence.
ACCESSION   DATBFZ010000206 DATBFZ010000000
VERSION     DATBFZ010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 7615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7615
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2757767"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2245)
                     /locus_tag="VH419_06180"
     CDS             complement(<1..2245)
                     /locus_tag="VH419_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011753959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEY7043238.1"
                     /translation="MFERFTDRARRVVVLAQEEARMLSHNYIGTEHILLGLIHEGEGV
                     AAKALESLNISLEAVRAQVEEIIGQGQQAPSGHIPFTPRAKKVLELSLREALQLGHNY
                     IGTEHILLGLIREGEGVAAQVLVKLGADLNRVRQQVIQLLSGYQGKETATAGAPAEGA
                     PSSSLVLDQFGQNLTQAAREGKLDPVIGRDKEIERVMQILSRRTKNNPVLIGEPGVGK
                     TTIVAGLAQNIVKGDVPETLKDKQIYTLDLGALVAGSRYRGDFEERLKKVLKEIRTRG
                     DIVLFIDEIHTLVGAGAAEGAIDAASILKPMLARGELQTIGATTLDEYRKHLEKDAAL
                     ERRFQPIQVAEPTIAHTIEMLKGLRDRYEAHHRVTITDEALVSAATLADRYISDRFLP
                     DKAIDLIDEAGSRLRIRRMTAPPDLREFDEKIADVRRRKEGAIDAQDFEVAASLRDEE
                     KKLILAKTEREKQWKAGDMDVVAEVDEELIAEVLATATGIPIVKLSEAESTRLLKMED
                     ELHKRVIGQDEAIKALSRAIRRTRAGLKDPKRPGGSFIFAGPSGVGKTWLSKSLAEFL
                     FGDEDALIQLDMSEFSEKHTVSRLFGSPPGYVGYEEGGQLTEKVRRKPFSVVLFDEVE
                     KAHPDIFNSLLQILEEGRLTDSQGRVVDFKNTVIIMTTNLGTRDIAKGVNLGFAQASD
                     QQGSYDRMKAKVTDELKQHFRPEFLNRVDEVIVFPPLTQDEIISMVDYMITSVEIRMK
                     DRDMALEL"
     gene            complement(2523..2840)
                     /locus_tag="VH419_06185"
     CDS             complement(2523..2840)
                     /locus_tag="VH419_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002512999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lsr2 family protein"
                     /protein_id="HEY7043239.1"
                     /translation="MAQRVHVVLEDDLDGSNADETVMFGLDGVNYEIDLSSKNATKLR
                     DALSKYVDNARRTHTRGRRSGGRRSSTNAASIREWARSHGYEVSDRGRVPADIKAAYD
                     AAH"
     gene            complement(2936..4393)
                     /gene="lysS"
                     /locus_tag="VH419_06190"
     CDS             complement(2936..4393)
                     /gene="lysS"
                     /locus_tag="VH419_06190"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020579470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEY7043240.1"
                     /translation="MRVRLEKRQRLIDKGHDPYPLSVPRTHELRDIVAAYDVEALGAD
                     HRTGDHVAVTGRVIFLRNTGKLCFARLRAGDGTELQAMFSLADLGDDALADFKALVDL
                     GDLIAVQGEVITSRRGELSVQAVTWQMAAKALRPLPNEHKPLSEEARVRLRYVDMIVR
                     PQPRDLVRGKAAVLKSLRSTLDELGFLEVETPVLQLTNGGASARPFHTHLNAFGQDMV
                     LRIALELDLKKAMVGGVERVYEIGRTFRNEGIDSTHAAEFSMLEAYQAYGDYDTMAAL
                     VQQLVVDAARTVERTVVPARAGGEIDLEAEWRHASILDLVSEVIGEEVTPETSAKKLR
                     KYAEQLEVSLQPEWGASDIVVELFEKLVEHTLMVPTFVRDYPEDAKPLAKPHRRIPGL
                     GEAFDLVINGVELAPAYSELNDPVLQRERLVAQSLLAAQGAEEAMELDETFLRALEYG
                     MPPAGGMGLGIDRLVMLLWGVGIRESILFPLLRPE"
     gene            complement(4559..5386)
                     /locus_tag="VH419_06195"
     CDS             complement(4559..5386)
                     /locus_tag="VH419_06195"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017933200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEY7043241.1"
                     /translation="MSVRNERTTIGLFDGDQLRQHWRVSTDERRTADEWGLLISGLIE
                     SHGQNEDTEQDDGHTKGEQRTATMHGEADGVVGLCISSTVPVVLHELRSMTLNFFPDA
                     HTVVVGPGVRTGLPVRMDNPREVGTDRVANAVAAAARWGGPVIVVDLGTAIVFEVVNT
                     AGQYVGGVISPGVDISVAALRERGAQLRQVEIEQPRTVVAKNTIEALQSGAVHGFAGL
                     VDGVVARIIDELDEGAPVVVATGALDSAVIEACSTITDREPYLTLYGLQMIYERNTR"
     gene            complement(5433..6227)
                     /locus_tag="VH419_06200"
     CDS             complement(5433..6227)
                     /locus_tag="VH419_06200"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY7043242.1"
                     /translation="MNHPKTLVSIPPFTDAATTVAIAVEAEAAGWDGVFLWDHLQWAP
                     QVRPPVHDPWVLLGAIAQVTEHVRIGTLVTPLSRRRLQVVAKHVLSLDHLSNGRVTFA
                     VGLGEPPVEDFAAFGDEADPRMRAAMLDEGLGVLDVLLRGERVDHHGEHYTAQAQLRP
                     GPVQRPRPPIWVAGVLPNRRPLARARRWDGVVPIGQGDLLTPADLVDYLHGEIPDGWD
                     VVASWAPEYGASEYAAAGATWLVESTMPVGDWVDEFRGRVRSGPGG"
     gene            complement(6238..7137)
                     /gene="panC"
                     /locus_tag="VH419_06205"
     CDS             complement(6238..7137)
                     /gene="panC"
                     /locus_tag="VH419_06205"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017933204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEY7043243.1"
                     /translation="MNAPVIARTRSELARLVNGPGANGTGANGPGATGSGNGRTSIAF
                     VPTMGALHHGHAALIDEARRRGEVVVVSIFVNPLQFAPGEDLERYPRTFDADVELCAR
                     HGADVVFAPAVDEVYPGGQPQVTVEPGALGTVLEGASRPGHFRGVLTVVAKLFGLVTP
                     DVAVFGQKDYQQLVLVRRMVADLCMAVDVVGTETVREPDGLALSSRNRYLSPSERSSA
                     LALSMALRAGQAAGRGGPAAVRREAERVMDSSRGVDVDYLELTGEDLSPAPTAGPARL
                     LVAARVGSTRLIDNMPVVLGTVG"
     gene            complement(7172..>7615)
                     /locus_tag="VH419_06210"
     CDS             complement(7172..>7615)
                     /locus_tag="VH419_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008354881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HEY7043244.1"
                     /translation="LAAELVGELGGRISWIDEQNRALYHAALAHGANHLVTLVSQAMD
                     VLRTAGSDDPAATLRPLLTAALDNVLDYGDAALTGPIVRGDVETVRTHLQRLVATGQP
                     RSTVESYVAMARATATHAVLDGRLEPRRAAELIELLDGPRTGVTG"
BASE COUNT         1224 a         2607 c         2444 g         1340 t
ORIGIN      
        1 tcagctcgag cgccatgtcg cggtccttca tccggatctc gaccgaggtg atcatgtagt
       61 cgaccatcga gatgatctcg tcctgcgtca gcggcgggaa cacgatgacc tcgtcaacgc
      121 ggttgaggaa ctccggccgg aagtgctgct tgagctcgtc ggtgaccttc gccttcatcc
      181 ggtcgtacga tccctgctgg tcgctggcct gggcgaatcc gaggttgacg cccttggcga
      241 tgtcgcgggt gccgaggttc gtcgtcatga tgatgacggt gttcttgaag tcgacgaccc
      301 ggccctgtga gtcggtcagc cgaccttcct ccaagatctg caacagcgag ttgaagatgt
      361 ccgggtgcgc cttctcgacc tcatcgaaca acacgacgga gaacggcttg cgccggacct
      421 tctcggtcag ctgcccgccc tcctcgtagc cgacgtagcc aggaggcgaa ccgaacagcc
      481 gcgagaccgt gtgtttctcg gagaactcgc tcatgtcgag ctggatcagc gcgtcctcgt
      541 cgccgaacag gaactccgcc agggacttcg acagccatgt cttgccgaca cccgaaggtc
      601 cggcgaagat gaacgacccg cctggccgct tggggtcctt caaccctgca cgagtacgcc
      661 ggatcgcccg ggacagcgcc ttgatcgcct cgtcctggcc gatgacgcgc ttgtgcagct
      721 cgtcctccat cttcagcagg cgagtggact cggcctcgct gagcttgacg atcgggatgc
      781 cggtcgccgt tgcgaggacc tcggcgatga gctcttcgtc gacctcggcg acgacatcca
      841 tgtcgccggc cttccactgc ttctcgcgct cggtcttggc gaggatgagc ttcttctcct
      901 cgtcgcgcag cgaagcggcc acctcgaagt cctgcgcgtc gatcgcgccc tccttgcggc
      961 ggcgtacgtc ggcgatcttc tcgtcgaact cgcgcaggtc cggcggtgcc gtcatccggc
     1021 ggatgcgcaa ccgcgacccg gcctcgtcga tcaggtcgat cgccttgtcc gggaggaacc
     1081 ggtcgctgat gtagcgatcg gcaagcgttg ccgccgagac cagtgcctcg tcggtgatcg
     1141 tcacccggtg gtgcgcctcg tagcggtcgc gcaacccctt gagcatctcg atcgtgtgcg
     1201 cgatcgtcgg ctcggccacc tggatgggct ggaaacggcg ttcgagtgct gcgtccttct
     1261 cgaggtgctt gcggtactcg tcgagggtcg tcgcgccgat cgtctgcagc tcgccacggg
     1321 ccagcatcgg cttgagaatg ctggcggcgt cgattgcgcc ctctgcagca cccgcgccca
     1381 ccagcgtgtg gatctcgtcg atgaacagca cgatgtcgcc gcgcgtgcgg atctccttca
     1441 gcaccttctt caagcgctcc tcgaagtcac cgcggtagcg agagccggcc acgagggcac
     1501 cgaggtcgag ggtgtagatc tgcttgtcct tgagcgtctc gggcacgtcg cccttgacga
     1561 tgttctgtgc gagaccggcc acgatcgtgg tcttgccgac gccgggttct ccgatcagta
     1621 ccgggttgtt cttcgtacgc cgcgacagga tctgcatcac ccgctcgatc tccttgtcgc
     1681 ggccgatgac cggatcgagc ttcccttcgc gagccgcttg ggtgaggttc tggccgaact
     1741 ggtcgagcac cagtgaggac gacggcgcac cttcggccgg cgcgccggct gtcgcggtct
     1801 ccttgccctg gtagccgctg aggagctgga tcacctgctg gcggacccgg ttgaggtcgg
     1861 cgccgagctt gaccagcacc tgggctgcga cgccctcacc ttcgcggatg aggccgagca
     1921 ggatgtgctc ggtgccgatg tagttgtgcc cgagctgcag cgcttcgcgc aggctcagct
     1981 cgagcacctt cttggcacgt ggtgtgaacg ggatgtgccc actcggtgcc tgctgtccct
     2041 ggccgatgat ctcttcgacc tgggcacgga cagcctccag tgagatgttc aggctctcca
     2101 acgccttggc tgcgacgcct tcaccctcgt ggatgagccc aaggagtatg tgctcggtcc
     2161 cgatgtagtt gtggctgagc atcctggcct cttcctgggc caggacgacg acgcgccgtg
     2221 cgcggtcggt gaaccgttcg aacatgtggc tgatcgctcc tcggttgtgc gttgctgtcc
     2281 atgctagtca atggcccgac gccgcatatc ggcacggacg aaggccggac gttcggccga
     2341 cgaccgctca gcgatcgagc ggaatcgggc aagcaggaca cagatcacaa ccacgggggg
     2401 cgcgacaatg ttccccaacc gttacgccgc aggcgaacac ctggcgttca gcgcgaccga
     2461 ggtggcactc ggcgtccgac acgccgagga gaatgcaaca aaacctgcca cctcggcgga
     2521 ggtcagtggg cggcgtcgta ggcggccttg atgtcggccg gaacgcggcc ccggtcggac
     2581 acctcgtaac cgtgcgagcg agcccactcg cggatgctgg cggcgttcgt cgaggagcga
     2641 cggccaccgg atcgacggcc acgggtgtgc gtacgccggg cgttgtccac gtatttcgac
     2701 agcgcatcgc ggagtttggt tgcattcttt gacgaaaggt cgatctcata attgacgcca
     2761 tcgagcccga acatgacggt ttcgtcggca ttgcttccgt cgagatcgtc ctcgagaacg
     2821 acgtgtacac gctgggccac ttgccatctc ctcgatccaa gaaaaaccga cgacttcacg
     2881 gtaacaggct cccgcacaaa gagcgagcga gagaattgaa gacgcagcgc gcagactatt
     2941 ccggccgcaa gagtgggaac agtatcgact ccctgatccc gacaccccac aacagcatga
     3001 ccagtcgatc aatcccgagc cccattccac ccgcgggtgg cattccgtac tcgagtgccc
     3061 ggaggaaggt ctcgtccagc tccatcgcct cctcggcccc ctgggcggcc agcaaggact
     3121 gagccacgag tcgttcccgc tgaagcacgg gatcgttcag ctccgagtac gccggcgcga
     3181 gctccacccc gttgatgacg aggtcgaacg cctcaccgag cccaggaatt ctccggtgcg
     3241 gcttggccaa tggcttggcg tcctccgggt agtcccggac aaatgtcggg accatcaacg
     3301 tgtgctcgac gagtttctcg aacagctcga cgacgatgtc gctcgcgccc cactcgggct
     3361 ggagcgagac ctcgagctgc tcggcgtact tccgcaattt cttcgcagaa gtctccgggg
     3421 tgacttcttc tcctatcact tccgaaacga gatcgagaat cgaggcatgc cgccattcgg
     3481 cctccaggtc gatctctcca ccggctcgcg cgggaacgac cgtacgctcc accgttcgcg
     3541 ccgcgtcgac gacgagctgt tggacaagcg ccgccatcgt gtcgtagtcg ccgtacgcct
     3601 ggtacgcctc cagcatcgag aactccgcgg catgggtgga atcgatgcct tcgttgcgga
     3661 acgtccgccc gatctcgtac acccgctcca cgccaccgac catggccttc ttgagatcga
     3721 gctcgagggc gatccgtagc accatgtcct ggccgaaggc gttcaggtgg gtgtggaacg
     3781 ggcgggcgct cgcgccgccg ttcgtgagct gcaggaccgg agtctccacc tcgaggaacc
     3841 ccagctcgtc cagcgtcgac cgcaacgact tcagcacggc tgccttgccc cggacgaggt
     3901 cgcgaggctg tggccgcacg atcatgtcga cgtaccgcag ccggacgcgc gcctcctccg
     3961 acaacggctt gtgctcgttc ggcagcgggc gtagcgcttt cgccgccatc tgccaggtga
     4021 ccgcctgcac ggacaactcg ccacgccggc tcgtgatgac ctccccctgc acggcgatga
     4081 ggtctccgag gtcgaccagc gccttgaagt ccgcgagcgc gtcgtcgccg aggtcggcca
     4141 gggagaacat cgcctgcagc tcggtgccgt cgccggcccg taaccgggcg aagcagagtt
     4201 tcccggtgtt ccgcaggaag atgacgcgtc cagtcacggc cacgtggtcg ccggtccgat
     4261 ggtccgcgcc aagtgcctcg acgtcgtacg ccgccacgat gtccctcagc tcgtgtgtgc
     4321 gggggacgga gagtggataa gggtcgtgcc ctttgtcgat cagccgctgc cgcttctcga
     4381 ggcgaacgcg catctgctcg ggcagctcgt ctggttcgtg ctcgtcgata aacccgtgcg
     4441 acgtcgcgaa aacctcgtcc tcgacaggct cgatcgacga gtcctcgctc atgccgctca
     4501 ggctacggca tcacgcgcgg gccgagctgt gcacgacggc gggcacacag agcgctgctc
     4561 aacgggtgtt gcgctcgtag atcatctgca atccgtacaa cgtgaggtag ggctcgcggt
     4621 cggtgatcgt gctgcaggct tcgatgaccg ctgagtcgag tgcgccggtg gcgaccacaa
     4681 ccggcgcccc ttcatcgagc tcgtcgatga tccgcgccac gacgccgtcg acgagaccgg
     4741 caaagccgtg caccgcaccg gactgaagtg cctcgatggt gttcttggcc accacggtgc
     4801 gtggctgctc gatctccacc tgccgcagct gtgcacctcg ttcgcgcaag gctgccaccg
     4861 agatgtccac ccctggcgag atgacgcctc cgacgtactg gccggcagtg ttgaccacct
     4921 cgaacacgat ggccgtgccg agatcgacga cgatcaccgg gccaccccac cgcgcggccg
     4981 ccgccacggc gttcgcgacc cggtccgtgc cgacctcacg agggttgtcc atccgtaccg
     5041 gcagacccgt cctgacgccc ggccccacga cgaccgtgtg tgcgtcgggg aagaagttca
     5101 gggtcatgga ccgcagctcg tgcagcacga ccggcacggt cgacgagatg cagaggccaa
     5161 ccaccccgtc cgcctcaccg tgcatggttg ctgttcgctg ttcgcccttg gtgtgaccgt
     5221 cgtcctgctc agtgtcctcg ttctgaccgt ggctctcgat gaggccgctg atgagcagac
     5281 cccactcgtc cgcggtacgc cgctcgtcgg tggacacccg ccagtgttga cgcagctggt
     5341 caccgtcgaa gaggccgatc gtggtgcgct cgttgcgcac gctcatgcac aacacgttca
     5401 ccaagccgct cctcttctcg actcatacgt gctcagccgc cgggaccgct cctgactctg
     5461 ccacggaact cgtcgaccca gtcgccgacg ggcatcgtcg actccacgag ccaggtcgct
     5521 ccagccgcgg cgtactccga cgcgccatac tccggcgccc agcttgcgac gacgtcccag
     5581 ccatcaggga tctcgccgtg cagatagtcc accaggtccg cgggggtcag caggtcaccc
     5641 tgcccgatgg gtacgacgcc gtcccatcgc cgcgcgcgag ccagtggtcg acgattcggt
     5701 agcacgccgg ccacccagat cggcgggcgc ggacgctgaa caggtccggg tcgcagctgc
     5761 gcctgcgcgg tgtagtgctc tccgtggtga tcgacccgct cgcctcgcag cagcacgtcc
     5821 agcacgccaa gaccctcgtc gagcatcgcc gcccgcatcc gcggatccgc ctcgtcaccg
     5881 aatgccgcga agtcctcgac cggcggttca ccgagcccca cggcgaaggt cactcgcccg
     5941 ttgctcagat gatccaggct cagcacatgc ttggcgacga cctgcaaccg tcgccgactc
     6001 agcggagtga ccagggtgcc gatccggaca tgctcggtca cctgagcgat cgcgcccagc
     6061 agcacccatg gatcgtgaac cggaggtctg acctgcggcg cccattggag gtggtcccac
     6121 aagaagacgc cgtcccaacc agccgcttcg gcctcgaccg cgatcgcgac ggtcgtggcg
     6181 gcatccgtga acggcgggat gctcaccaac gtcttcgggt ggttcatcgc acacacctca
     6241 acccaccgtc cccagcacga ccggcatgtt gtcgatcagt ctcgttgacc cgacccgggc
     6301 cgccaccagc agtctcgcgg gtccggccgt cggtgctggt gacagatcct cgccggtgag
     6361 ctccaggtag tcgacgtcga ccccgcgaga tgagtccatc acccgttcgg cttcacggcg
     6421 tacggcggcc gggccgccgc ggccggccgc ctggcccgcg cggagtgcca tcgacagcgc
     6481 gagcgcgctg gagcgttccg acggcgagag atagcggttg cgcgagctca gcgcgaggcc
     6541 gtcgggctca cgcacggtct ctgtcccgac cacgtcgaca gccatacaga ggtcagccac
     6601 catccgtcgg acgaggacca gctgttggta gtccttctgt ccgaagacgg caacgtcggg
     6661 agtcacgaga ccgaagagct tcgcgacgac cgtcagtacg ccgcgaaagt ggcccggccg
     6721 actggctcct tccagcacgg tccccaacgc cccgggctcg accgtgacct gcggctgtcc
     6781 gcccgggtag acctcgtcga cggcgggggc gaagacgacg tcggccccgt gcctggcaca
     6841 cagctccacg tcggcgtcga acgtccgtgg gtagcgttcg aggtcctcac cgggagcgaa
     6901 ctggagcggg ttgacgaaga tcgagacgac cacgacctcg cctcgacgtc gggcctcgtc
     6961 gatgagtgcg gcgtgaccgt ggtggagggc gcccatcgtc gggacgaagg cgatcgaggt
     7021 tcggccattg cctgatccag tggcgccagg tccattggcg cctgttccat tggcgcctgg
     7081 tccgttgacg agtcgggcca gctcggatcg cgtcctcgcg atcaccgggg cgttcatcgc
     7141 gtgaccgctc gaccttcgta cgccggcagg ctcaacccgt cacgccggtg cgcgggccgt
     7201 cgaggagctc gatcagctcc gcggcccgcc gtggctcgag ccggccgtcg aggaccgcgt
     7261 gggtcgccgt cgcgcgcgcc atcgcgacgt acgactcgac cgtcgagcgg ggctggccgg
     7321 tggcgacgag gcgctgcaga tgagtccgca cggtctcgac gtcgcctcgg acgatcggtc
     7381 cggtcagagc cgcgtcgccg tagtcgagga cgttgtccag agctgctgtc agcaacggcc
     7441 gcaaggtggc cgcgggatcg tcggatccag ccgtgcgcaa gacgtccatc gcctgcgaca
     7501 cgagggtgac caggtggttg gcgccgtgcg ccaaggccgc gtggtagagc gcgcggttct
     7561 gctcgtcgat ccaggagatc cggccgccga gctcgccgac gagctcggcg gcgag
//
LOCUS       DATBFZ010000207         2856 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_4993051, whole genome shotgun sequence.
ACCESSION   DATBFZ010000207 DATBFZ010000000
VERSION     DATBFZ010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2856
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4993051"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2287
                     /locus_tag="VH419_06215"
     CDS             <1..2287
                     /locus_tag="VH419_06215"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEY7043245.1"
                     /translation="DHFDMINLSVGSDDGTVDDPENAFVAKLAANGVLPVISMGNGGD
                     NTDIGGAPGNAPASLAVASSVDSYQLRDGVRVNDPSDVAGVAAGQFSIAYDWPNNGPS
                     GDPVTGDVVKIPTSGAFDDPGTGNNTDGCDALSDEEAAAVAGKVVWLYWDDNDATRRC
                     GSAARGGNVAAAGAIGAIFTSSLDVFNAGITGVADIPIIQLPKAQTDRLTPAVDAGTL
                     NVTFDGALQASVKDITPSISDTLSSFSSRGPHGSIGVVKPDVTAPGDSIASAGMGTGN
                     NVLVISGTSMASPLVAGVAALVKSKHPSWTPQMLKAAVMNTATHDLWSQPGHSGDRYG
                     PARVGAGRVDARAAVRTKSITYVKGPNQTVSASFGVVPALIDGGTVVKHKTLVVRNLG
                     TKPTTYGLAYDAVNPSPGVSYSLSRSSITVPANSQKTVTVTMRVHPGQLAHTLDPTMS
                     FSQSTGFGDLPRTYVADSSGHLTVTPQGKKELRVPVYGAAKPVTDTTASSPQPGEIDI
                     AGEGVDQGTSLEDAYIGFASIMQLGATSPQQPKCTVSIRSNCWTNRSDRAGDIQYVGA
                     GTGQAFGDPFLYFGISTHQDWANLNVMTPYVDVDTDGDDFPDIEIYAQNYASAQGRTD
                     VLLAYTVDYKTGDILDIEAANLAQFDVDTNVYDTNVIMLPMDLTFVADGGPDLTGPDA
                     EINYWGATANGYTGHDQDRTGEISYNVGSPDLVADADPIFEDGEQTVPVEGSGDALVF
                     HYRGAAGHRAEVVNVPAPPSP"
     gene            complement(2399..>2856)
                     /locus_tag="VH419_06220"
     CDS             complement(2399..>2856)
                     /locus_tag="VH419_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEY7043246.1"
                     /translation="IQGWMGHADPFLMGPGYTPARGMRRFISGTPPILGMVAMQDMLA
                     LVEEAGIEAIRAKSVALTSYAVELADEHLAAYDVTVRSPRDPGRRGGHVTLHHPRMRD
                     VVASLWERDVIPDYRDPGGLRIGLSPLSTSFDELERGMDTVRRVVTALD"
BASE COUNT          481 a         1008 c          957 g          410 t
ORIGIN      
        1 cgaccacttc gacatgatca acctgtcggt gggctcggac gacgggacgg tcgacgaccc
       61 ggagaacgcg ttcgtggcca agctcgccgc gaacggcgtg ctcccggtca tctcgatggg
      121 caacggcggc gacaacaccg acatcggtgg cgccccgggc aacgcacccg cgtcactcgc
      181 ggtggcgagc tcggtcgact cctaccagct gcgcgacggt gtccgcgtga acgacccgtc
      241 ggacgtcgca ggcgtggcgg cgggtcagtt ctcgatcgcc tacgactggc ccaacaacgg
      301 cccgagcggc gacccggtca ccggtgacgt cgtgaagatc ccgacctcgg gcgcgttcga
      361 cgatcccggc accggcaaca acaccgacgg ctgcgacgca ctctcggacg aggaggccgc
      421 agcggtcgcc ggtaaggtcg tgtggctgta ctgggacgac aacgacgcga cccgtcgttg
      481 tggctcggca gcgcgcggcg gcaacgtcgc cgccgccggt gcgatcgggg cgatcttcac
      541 ctcgtctctc gacgtgttca acgccgggat cacgggtgtc gcggacatcc cgatcatcca
      601 gctgcccaag gctcagaccg accggctcac cccggccgtc gacgccggca cgctgaacgt
      661 gacgttcgac ggtgccctgc aggcgtccgt caaggacatc accccgtcca tcagtgacac
      721 cttgtcctcc ttctcctcgc gcggcccgca cgggtcgatc ggcgtggtga agccggacgt
      781 cacggctccc ggcgactcca tcgcctcggc cggcatgggc acgggcaaca acgtcctggt
      841 gatctccggt acgtcgatgg cgtcaccgct ggtggccggt gtggccgctc tggtgaagtc
      901 caagcacccg tcgtggactc cgcagatgct caaggcggcg gtcatgaaca ccgcgaccca
      961 cgacctgtgg agccaacccg gccactcggg tgaccgctac ggcccggccc gggtcggggc
     1021 gggtcgcgtg gacgcgcgtg cggccgtccg gacgaagtcg atcacctacg tcaagggtcc
     1081 gaaccagacc gtctcggcga gcttcggggt cgtcccggcg ctcatcgacg gcgggacggt
     1141 ggtgaagcac aagaccctcg tggtccgcaa cctcggcacc aagccgacga cgtacggcct
     1201 cgcctacgac gcggtgaacc cgtccccggg cgtgtcctac tcgctgtcga ggtcgtcgat
     1261 cacggtcccg gccaacagcc agaagacggt caccgtcacg atgcgggtcc acccgggtca
     1321 gctggcgcac accctcgacc cgacgatgag cttcagccag tcgaccgggt tcggggacct
     1381 gccgcggacc tacgtcgcgg acagctccgg tcacctgacg gtgacgccgc agggcaagaa
     1441 ggagctccgg gtgccggtgt acggggcggc caagcccgtc acggacacca cggcgagctc
     1501 gccgcagcct ggcgagatcg acatcgcggg tgaaggcgtc gaccagggca cctcactgga
     1561 ggacgcctac atcggcttcg cctcgatcat gcagctcggg gcgacgagcc cgcagcagcc
     1621 gaagtgcacg gtgagcatcc ggtccaactg ctggaccaac cggtccgacc gggccggtga
     1681 catccagtac gtcggagcgg gcacgggtca ggccttcggc gacccgttcc tgtacttcgg
     1741 catctccacc caccaggact gggccaatct caacgtgatg actccgtacg tcgacgtcga
     1801 caccgacggc gacgacttcc cggacatcga gatctacgcc cagaactacg cgtccgccca
     1861 gggccgcacg gacgtgctgc tcgcgtacac ggtggactac aagaccggtg acatcctcga
     1921 catcgaggcg gccaacctcg cgcagttcga cgtcgacacg aacgtgtacg acacgaacgt
     1981 gatcatgctg ccgatggacc tgaccttcgt cgccgacgga ggtccggacc tgacgggtcc
     2041 ggacgcggag atcaactact ggggcgcgac ggccaacggc tacaccggcc acgaccagga
     2101 ccggaccgga gagatcagct acaacgtcgg gtcgcccgat ctcgtagcgg atgccgatcc
     2161 gatcttcgag gacggggagc agacggtccc cgtcgagggg agcggtgacg ccttggtgtt
     2221 ccactaccgc ggcgcagccg gccaccgagc cgaggtggtc aacgtcccgg caccgccctc
     2281 tccgtgaggt accacgtcgg gatcgacgtc tgatcccgtc tgacagctga acacgaaggg
     2341 ccctggccga cggccagggc ccttcgtcgt gttctcgtcg cgtgcgtcgt cgtggtggtc
     2401 agtcgagcgc cgtgacgacc cgacgcaccg tgtccatccc acgctcgagc tcgtcgaacg
     2461 acgtcgagag cggcgagagc ccgatgcgga gaccgccggg gtcacggtag tccgggatca
     2521 cgtcgcgctc ccacaaggac gccacgacgt ctcgcatccg cggatggtgc agggtgacgt
     2581 gcccaccccg tcgaccgggg tcgcgtggcg accgcacggt cacgtcgtac gccgcgaggt
     2641 gctcgtcggc gagctcgacg gcgtacgacg tcagggcgac ggacttggcc cggatcgcct
     2701 cgatgccggc ctcctcgacc agggcgagca tgtcctgcat ggcgaccatc ccgaggatcg
     2761 gcggggtccc ggagatgaac ctccgcatgc cgcgcgccgg ggtgtagccc ggccccatca
     2821 ggaacgggtc ggcgtggccc atccacccct ggatcg
//
LOCUS       DATBFZ010000208         4868 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_4072633, whole genome shotgun sequence.
ACCESSION   DATBFZ010000208 DATBFZ010000000
VERSION     DATBFZ010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 4868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4868
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4072633"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1205
                     /locus_tag="VH419_06225"
     CDS             <1..1205
                     /locus_tag="VH419_06225"
                     /inference="COORDINATES: protein motif:HMM:NF012892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEY7043247.1"
                     /translation="LGGRGLRVLAAARDVAAADADTLAPTDLETGLQLVGVVGIVDPP
                     RPEAIEAIALCRTAGVHVAMITGDHAGTALAISREMGIADDSSRVVIGSELEEMSDAQ
                     LRDIVRDVDVYARTSPEHKLRIVAALQSHDEVVAMTGDGVNDAPALTQADVGVAMGIK
                     GTSATKEAADIVLADDNFATIERAVEEGRRIYDNIRKSILFLLPTNGAQSLVILVAVL
                     FDLTMPLQPVQVLWINLVTSITLSLALAREPAELGVMRRPPRDARASIITPAFLRHIL
                     IVSVIIGGATLGVFSFELARHESLPQVQATAVTMLAFGQLAFLFSCRFLDRSSITLDV
                     LRGNRAVWWCATALVILQAAFVYAPPMQQLFDVAAIGPREWALIFGLSVVVFLLAEAA
                     KAVDRRRT"
     gene            1519..4836
                     /locus_tag="VH419_06230"
     CDS             1519..4836
                     /locus_tag="VH419_06230"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEY7043248.1"
                     /translation="MIDDVLRAGGWPLLLEAGVTDAEARAFAVPTGTVTFLLTDVESS
                     TRGWEASPDAMTVAIARHYELLDGAISAHGGVRPVEQGEGDSVVGAFGRSSDAIAAAL
                     AAQQALLSEPWPEGGTLRVRMAIHTGEAQLRGDGNYFGQPVIRCARIRATGHGGQVLL
                     SDATAALVADHLPADAALLDLGLHRLKDLGRPDRVWQLVHPDLPAEHPPLRSLDSFRH
                     NLPSQVTPLIGRVADIEAVTALVDTERLVTLTGPGGVGKTRLAMAVCAEVIERWPGGV
                     LWVELATVLEQDAVGRAVLAALRCPEVPGVTPAQQLAGELAEGPALIVLDNCEHLLDG
                     CGRFVMSMLAALPSVSILATSREPVGVPGEVIWPVPSLGVPPTERSLDVPSLSQYDAV
                     RLFVDRAHRARPSFVVNDANAPAIAQVCHRLDGIPLAIELAAARCRQMTAEHIERELD
                     NRFRLLTGGTRGVVARQQTLMASIDWSFERLDEVEQRIFRRLGVFVGSFPLEAAEAIA
                     SSCSDVEPVEVFDALSHLVDKCLVVVGEDRDGEPLYRQLETIRAYAVERARQAGELAQ
                     LQASHAQWWTSWLSDRWLVLHTEAVADVVEQFHGNVASALLWSVSTPELGLRLLFLVC
                     RAWLGTSRLGDAMTAFDALITPDNARLHGPAWRDAALAGAPFVVAARGYEVFVALLNQ
                     VKAEAEAASDRYHEVRAAWMLSMTDTVSLELRDLAREHGDPYLQTIATVTAAQWRALN
                     DPLEAQDLLREATLVAESSGNRFARDWVTRVRGYLARDTGDLAHAMELAETLIDRGAV
                     ERGLPLLISAAFLAADADRLAGAVTTAEAHSRRMPGEHHLLARAIRRQQLLTDDAVSA
                     VDDDLDNWIPTSGTLWVDCREAIAAGAADEAVAAVERHDYDSPHSNVVRSAILAATTG
                     SEDAWHEALRVAVEHDLRLISADALEGIAVAAAESESWTECLRLAGAAERLRDETGYR
                     WRFAPERDALAGALQAARTALSPDEAAAALLAGRNLDDRAAAAFAARSRGTRKRPKLG
                     WASLTPTELEVVALVSQGLTNPQIAGQLLMGRATVKTHLDHIFVKLGVQTRTEVAAAA
                     IRREATQQRRSPAASAP"
BASE COUNT          741 a         1698 c         1667 g          762 t
ORIGIN      
        1 agctgggtgg gcgtggtctt cgggtcctcg cggccgcccg tgacgtcgcc gccgccgatg
       61 cagacaccct tgcgcccact gacctcgaga ccgggctcca gctcgttggc gtggtcggga
      121 tcgtcgaccc gccccggccg gaggccattg aggcgatcgc gttgtgccgt acggctggcg
      181 tgcacgtcgc gatgatcacc ggtgaccacg ccggcacggc tctcgcgatc tcgcgcgaga
      241 tgggaatcgc cgatgacagc tcccgagtcg tgatcggaag cgagctcgag gagatgtcag
      301 acgcgcagct gcgtgacatc gtccgagacg tcgacgtcta cgcgcgcacc agcccggagc
      361 acaagcttcg gatcgtcgcc gcgctccagt ctcacgatga ggtcgtggcg atgacaggag
      421 acggcgtgaa cgacgcccct gcactgacgc aggccgatgt tggcgttgcg atggggatca
      481 agggcacatc ggccaccaag gaggccgccg acatcgttct cgccgacgac aacttcgcca
      541 cgatcgaacg agccgtggaa gagggacggc ggatctacga caacatccgc aagtccatcc
      601 tcttcctgct gccgacgaac ggcgcacagt cgctggtcat cctcgttgcg gtgctgtttg
      661 acctcacgat gccgctccag cccgtgcagg tgttgtggat caacctggtc acgtccatca
      721 cgctgtcact ggcgctcgcg cgtgagccgg ctgagctcgg cgtgatgcgc agaccgccgc
      781 gggacgcgcg ggcgtcgatc atcactcctg cattcctgcg ccacatcctc atcgtgtcgg
      841 tgatcatcgg aggagccacg ctgggcgtgt tctccttcga gctcgcgcgc cacgagtcgc
      901 tgccccaggt ccaggcgacg gccgtcacga tgctggcgtt cgggcagctc gccttcctgt
      961 tcagctgccg gttcctcgat cgttcgagca tcacgctcga cgtgctgcga gggaaccgcg
     1021 ccgtgtggtg gtgtgccacg gctctggtga tcctgcaggc ggctttcgtg tacgcgcctc
     1081 cgatgcagca gctgttcgac gtcgcagcga tcggcccgcg cgaatgggcg ctcatcttcg
     1141 ggctgtcagt cgtcgtcttc ctcctcgcgg aggcagccaa agcggtcgac cggcggcgca
     1201 cctgacgagc ccccggtcgg cgtcacctca acggagaccg gtgcgactcg aggccgagat
     1261 cgtgaccgcc gacgctgaga tcctgaccgc cagcacggac gagcaccgcg ctacgacagc
     1321 cccaacctgt tccggtccaa ccacaacatc ccactgccct agccgactcg accggttagg
     1381 gccgccggcc ggtgtaccaa gaaccgtcgg cccgactccg tggggaccac agcgagcaga
     1441 gatgagtccc ctggcggcgt gggcttggtc ccccacatgg tggatgttcc ggaggcgggg
     1501 tcgcgggacg ctcgccgcat gatcgatgat gtgcttcgtg ccggagggtg gccgctgctg
     1561 ttggaggccg gagtgacgga cgcggaggcg cgagcgttcg cggtcccgac gggcaccgtg
     1621 accttcctgc tcaccgacgt cgagagttcg acccgcgggt gggaggcgtc gccggatgcg
     1681 atgaccgtgg ccatcgcccg ccactacgag ctgctggacg gggccatctc ggctcacggt
     1741 ggggtccggc ccgtagagca aggtgagggc gacagcgtgg ttggggcgtt cgggcgctcg
     1801 tccgatgcga tcgccgccgc gctggccgcc caacaggccc tgctgtccga gccttggccg
     1861 gagggcggga cgctacgggt gcggatggcc atccacacgg gcgaggcgca actccgaggc
     1921 gatggcaact acttcggcca gccggtcatc cggtgcgcac gcatccgtgc cacgggccac
     1981 ggcggtcagg tgctgctgtc ggatgccacc gcggcgctcg tcgccgacca cctgccggcg
     2041 gacgctgcgc tcctcgatct cgggctgcac cgtctcaagg acctcggtcg accggaccgg
     2101 gtgtggcagc tcgttcaccc tgacctccct gccgagcacc caccgctgcg atcactcgac
     2161 tcgttccggc acaacctccc atcacaggtc acacccctga tcggacgcgt cgcggacatc
     2221 gaggcggtca cagcgttggt cgacaccgag cgcctggtga cgctgacggg accggggggt
     2281 gtcggcaaga cgcgcttggc gatggcggtc tgcgccgagg tgatcgagcg atggcccgga
     2341 ggcgtgctgt gggtcgagct ggccacggtg ctcgagcagg acgctgtcgg tcgggccgtc
     2401 ctcgccgcgt tgcgctgtcc tgaggtcccc ggggtgacgc ctgctcagca gctggccggc
     2461 gagctggcgg agggccccgc gctcatcgtg ctggacaact gcgagcacct cctcgacggc
     2521 tgcggccgct tcgtgatgtc gatgctcgcc gccctaccca gcgtgtcgat cctcgccacc
     2581 agcagggaac cggtcggcgt gccgggcgag gtgatctggc cggtcccgtc acttggcgtg
     2641 cctccgaccg agcggtccct ggatgtcccg tcgttgtcgc agtacgacgc cgtgcggctc
     2701 ttcgtcgacc gcgcccaccg cgcccggcca tccttcgtcg tgaacgacgc caacgcaccg
     2761 gcgatcgcgc aggtctgcca tcggctcgac ggcataccgc tggccatcga gttggccgcc
     2821 gcccgatgcc gccagatgac tgcggagcac atcgagcggg agctcgacaa ccggttccgc
     2881 ctgctcacgg gtggcactcg gggggtggtc gcccgtcagc agacgctgat ggcgtcgatc
     2941 gattggagct tcgagcgcct cgacgaggtc gagcagcgga tcttccgtcg cctgggtgtg
     3001 ttcgtcggat ccttcccgct ggaagcagcc gaagccatcg cctcctcgtg cagcgacgtc
     3061 gagcccgtcg aggtgttcga cgcgttgtcg cacctcgtcg acaagtgcct ggtggtcgtc
     3121 ggcgaagatc gggacggcga accgttgtat cggcaactcg agacgatccg ggcctacgcg
     3181 gtcgaacgag ctcgtcaggc cggcgagctc gcgcagctgc aggcgtcgca cgcgcagtgg
     3241 tggacgtcgt ggctctcgga ccgctggctc gtgctccaca cggaggcggt cgccgacgtc
     3301 gtcgagcagt tccacggcaa cgtcgcgtct gcgctgctct ggagcgtgag cacacccgag
     3361 ctcggcctgc gcttgctgtt cctggtgtgt cgggcgtggc tgggcaccag ccgactcggc
     3421 gatgccatga ctgccttcga tgccttgatc acgcccgaca acgcgaggct gcacgggccg
     3481 gcatggcgcg atgcagccct cgccggggcc ccgttcgtcg tggccgcgcg cggctacgag
     3541 gtgttcgttg ctctcctgaa ccaagtgaaa gcggaggccg aagcagcgag cgaccggtat
     3601 cacgaggtgc gggccgcttg gatgctctcg atgaccgaca ccgtctcgct cgaacttcgc
     3661 gacctggccc gcgagcacgg cgatccgtac ctccagacga tcgccaccgt caccgccgcg
     3721 cagtggcggg cactcaacga cccgctcgag gcgcaggacc tcctcaggga agccacgctc
     3781 gtggccgaga gctccggtaa ccgatttgct cgggactggg tcacgagggt tcggggctat
     3841 ctcgcccggg acaccggcga cctcgcgcac gccatggagc tggccgagac gctcatcgac
     3901 cgtggcgcag tggaacgtgg ccttccgctg ctcatcagcg cggccttcct ggccgcagac
     3961 gcagaccgac tggcaggcgc cgtgacgaca gccgaagccc attcgcgacg catgcccggc
     4021 gagcaccatc tgctggccag agccatccgt cggcagcaac tgctcaccga cgacgcggtc
     4081 agcgcggtcg acgacgacct ggacaactgg atcccgacca gcggaacact gtgggtcgac
     4141 tgccgcgaag cgatcgcggc cggcgcggcc gacgaggccg tcgccgcggt cgagcggcat
     4201 gactacgaca gcccgcacag caacgtcgtg cggagcgcca tactcgccgc caccactggc
     4261 agcgaggacg cgtggcacga agcgcttcga gtcgcggtcg agcacgactt gcgactcatc
     4321 tcggccgacg ccctggaagg catcgctgtg gcggcggctg agtccgagag ctggacggag
     4381 tgcctgcggc ttgccggtgc ggcagaacgg ctccgcgacg agacgggcta ccggtggcga
     4441 ttcgcgcccg agcgcgacgc cttggcggga gccctccagg ccgcccgcac ggcactgagc
     4501 ccggacgagg cggcggccgc cctccttgct ggacgaaacc tcgacgatcg ggcagcggcc
     4561 gcgtttgccg cccgatcacg aggcacgcgc aagcggccga agctcggctg ggccagcctc
     4621 acgccgaccg agctcgaggt ggtcgcgctg gtgtcgcagg ggctcaccaa ccctcagatc
     4681 gccggccagc tcctcatggg ccgagccacc gtcaagaccc acctcgacca catcttcgtc
     4741 aagctcggcg tacaaacccg caccgaagtc gcggccgccg ccatccggcg agaagcgact
     4801 caacagcgac gctcacccgc cgcgtcggcg ccctgaagca ttcggccgca acaacctgac
     4861 ggccgaag
//
LOCUS       DATBFZ010000209         2713 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_3090907, whole genome shotgun sequence.
ACCESSION   DATBFZ010000209 DATBFZ010000000
VERSION     DATBFZ010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3090907"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(99..1238)
                     /locus_tag="VH419_06235"
     CDS             complement(99..1238)
                     /locus_tag="VH419_06235"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEY7043249.1"
                     /translation="MILAACGSDNSGSSTAATSAAATTAAATTASSAAATSAAATTAA
                     GGAATSAAGGGSAAGCTVGVSWNNYQEERWAKWDEPAIKAAVEAGGGKYISNDAKSSA
                     ETQASNVENLISQGAKVLIILAQDGTAIKPSVANAVANGVPVIAYDRLIEDPKALYIT
                     FDNVEIGRMEAREIFKAVPKGNYVIMKGNKADANADFLRGGYEEIIGAAVKSGDIKIV
                     GETYTDNWEPSKAQTQMEQFLTANNNNVQAVLSENDGMAGGVVAALQAQGLAGKVPVS
                     GQDGDQPALNRIALGTQHVDVWKDARLLGKTAGDAAIALCKQADASKVANAKPFTTPG
                     GIQVSSVLLTPQPITKDNLKVVLDAGWTTKENVCKGVAAGSVSVC"
     gene            1418..>2713
                     /locus_tag="VH419_06240"
     CDS             1418..>2713
                     /locus_tag="VH419_06240"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HEY7043250.1"
                     /translation="MPDQPARVVLGAAVASVIALLAACGSSGSSAGAPPRFVDDTSVS
                     GIDHQYTGGFEYFVGGGVAAFDCDDDGRADLYLAGGEAPAVLYHNDSPTGGALRFSPI
                     PSPVTDLAAVTGAYPLDVDSDGHIDLVVLRRGGDVVLRGLGGCRFENATEALGLDVPP
                     GWTTAFSATWEGANALPTLAFGRYLVPDQDRCDTSWLVRPDASGARYAPPIALSPGYC
                     TLSMLFSDWSHSGHRDLRVSNDRHYYIDGEEQLWRIVPGQPPTVYTKADGWQPLQIWG
                     MGIASQDITGDGRPEVFLTSQGDNKLQTLATDSARPDYHDVALDRGVTAQRPFVGGDI
                     LPSTAWHPEFADVNNDGWPDLLVTKGNVDAQIEQASRDPSNLFIGRPDGSFTEGAEAA
                     GIVDYERSRGAAVVDLNLDGLPDLVIVHRQANVTLWRNAG"
BASE COUNT          365 a          976 c          888 g          484 t
ORIGIN      
        1 cgtcgtcatc cgatccccct ggcccgtgcg tcgagtcgag cgtgtccccc gcccgcggtc
       61 gggcgagggg cacgcgagta catgtcagac cgacagggtc agcagaccga caccgagccg
      121 gcggccacgc ccttgcacac gttctccttc gtcgtccagc cggcgtcgag gacgaccttg
      181 aggttgtcct tggtgatcgg ctgcggggtc aggaggaccg acgacacctg gatgccaccg
      241 ggcgtcgtga acggcttggc gttggcgacc ttcgacgcgt cggcctgctt gcaaagtgcg
      301 atcgccgcgt caccggcggt cttgccgagc agacgggcgt ccttccagac gtcgacgtgc
      361 tgcgtgccga gcgcgatccg gttcagggcc ggctgatcgc cgtcctggcc cgacaccggg
      421 accttgccgg cgagaccctg ggcctgcagc gctgccacca cgccgcccgc catgccgtcg
      481 ttctcggaca gcacggcctg gacgttgttg ttgttcgccg tcaggaactg ctccatctgc
      541 gtctgggcct tcgacggctc ccagttgtcg gtataggtct caccgacgat cttgatgtca
      601 ccggacttca cggcggcgcc gatgatctcc tcgtagccgc cccgcaggaa gtcggcgttg
      661 gcgtcggcct tgttgccctt catgatgacg tagttgccct tcgggacggc cttgaagatc
      721 tcccgcgcct ccatgcgccc gatctcgacg ttgtcgaagg tgatgtacag cgccttcggg
      781 tcctcgatca ggcggtcgta ggcgatcacg ggcacaccgt tggcgaccgc gttcgccacc
      841 gacggcttga tggccgtgcc gtcctgggcc aggatgatca ggaccttggc gccctgcgag
      901 atcaggttct cgacgttgct ggcctgggtc tcggccgacg acttggcgtc gttggagatg
      961 tacttcccac cgccggcttc gacggcggcc ttgatggcgg gctcatccca cttggcccag
     1021 cgttcttcct ggtagttgtt ccacgacacg ccgaccgtgc agccggcggc gctgccgcct
     1081 cctgctgcgc tcgtggcggc gccgcccgcc gccgtggtgg cggccgcgct ggtggccgcg
     1141 gcactgctgg ccgtggtggc ggccgccgtc gttgctgctg cactggtggc ggccgtactg
     1201 ctcccgctgt tgtcgctgcc acacgcggcg aggatcatcg tcagcccaat acctgcggct
     1261 gcaatacctc gtacgcgcac cctcacattc ccccgcatgc ccatgttccc cctcactggt
     1321 ggtttcccgt tgcgtcccag tcggacgtcc cgggccaccg gactatatcc gggaacctgc
     1381 gactgctcgg tcgatcgagc tgtggttcaa tccgttcgtg cctgaccagc cggctcgcgt
     1441 cgtgctcggg gctgcggtcg cttcggtcat cgccctcctt gccgcgtgcg gaagcagcgg
     1501 ctcctcggcc ggcgcgcctc cgcggttcgt cgacgacact tccgtgtcgg gcatcgacca
     1561 tcagtacacc ggtggtttcg agtacttcgt gggcggcggc gtggccgcct tcgactgcga
     1621 cgacgacggt cgggccgacc tgtaccttgc cggcggcgag gcaccggccg tgctgtatca
     1681 caacgacagt ccgaccggtg gtgcccttcg gttctcgccc attccctcac ccgtcaccga
     1741 cctcgccgcg gtcacgggcg cctatccgct cgacgtcgac agcgacggtc acatcgatct
     1801 cgtcgtgctc cgccgcggcg gcgacgtcgt gctgcgggga ctcggtggct gccggttcga
     1861 gaacgccacc gaggcgctgg gcctcgacgt gccgcccggt tggaccacag ccttcagcgc
     1921 cacctgggaa ggcgcgaacg cgctgccgac gctcgccttc ggccgctacc tcgttcccga
     1981 ccaggaccgg tgcgacacga gttggctggt gcgacccgac gcgtcgggcg cccgctacgc
     2041 gcccccgatc gcgctgtcgc ccgggtactg caccttgtcg atgctgttca gcgactggag
     2101 ccactcgggg caccgcgacc tgcgcgtctc gaacgaccgc cactactaca tcgacgggga
     2161 ggagcaactg tggcggatcg tgccgggcca gccgccgacg gtgtacacga aggccgacgg
     2221 ctggcagccg ctgcagatct ggggcatggg catcgccagc caggacatca ccggcgacgg
     2281 acgccctgag gtgttcctca ccagccaggg ggacaacaag ttgcagaccc tcgccaccga
     2341 ctcggctcga cccgactacc acgacgtcgc cctcgaccgg ggcgtgacgg cgcagcggcc
     2401 cttcgtcggt ggcgacatcc tgccgtcgac ggcgtggcat cccgagttcg ccgacgtgaa
     2461 caacgacggc tggccggacc tgctggtcac caaaggcaac gtcgacgccc agatcgagca
     2521 ggcgagccgc gatccgagca accttttcat cggccggcct gatggctcct tcaccgaagg
     2581 ggccgaggcc gccggcatcg tcgactacga acggtcgcgc ggcgccgccg ttgtcgacct
     2641 caacctcgac ggcttgccgg acctcgtgat cgtccatcgt caggccaacg tcacgctgtg
     2701 gcgcaacgcc ggc
//
LOCUS       DATBFZ010000210         7335 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_1447690, whole genome shotgun sequence.
ACCESSION   DATBFZ010000210 DATBFZ010000000
VERSION     DATBFZ010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 7335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7335
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1447690"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..127
                     /gene="prfB"
                     /locus_tag="VH419_06245"
     CDS             <1..127
                     /gene="prfB"
                     /locus_tag="VH419_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754726.1"
                     /note="recognizes the termination signals UGA and UAA
                     during protein translation a specificity which is
                     dependent on amino acid residues residing in loops of the
                     L-shaped tRNA-like molecule of RF2; in some organisms
                     control of PrfB protein levels is maintained through a +1
                     ribosomal frameshifting mechanism; this protein is similar
                     to release factor 1; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEY7043251.1"
                     /translation="QMVKDLRTEHEVSNPTAVLDGDIDDFIESAIRWRRMAAAAN"
     gene            178..702
                     /locus_tag="VH419_06250"
     CDS             178..702
                     /locus_tag="VH419_06250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043252.1"
                     /translation="MSAIHATSSQDLLALYLNDHLLGASGGVELFRRAARGQSGDAAR
                     TRLEVLTRDVEQDRDALVDLMKQLDVRPARHRVALGWLGEKVGRLKLNGSLLSRTPLT
                     DLVELEYLRLGVEGKLAGWEAVEVAAGADHRIDLARVEELQQRAKDQLEALEELRREA
                     ARHAFAGRSEGPQK"
     gene            769..1719
                     /locus_tag="VH419_06255"
     CDS             769..1719
                     /locus_tag="VH419_06255"
                     /inference="COORDINATES: protein motif:HMM:TIGR01188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY7043253.1"
                     /translation="MAEHAITVEGLVKNFGEVHAVRGIDFTVSEGEMFGFLGPNGAGK
                     STTIKILCTLADPTQGAATVAGYDVVTQRSLVRRSIGLVFQEPTLDAYLTAEQNLSFH
                     GELYGVPRAALKKRIDAVLEMVGLADRRKDKVQTYSGGMKRRLEIGRGLLHSPRVLFL
                     DEPTIGLDPQTRASIWDYVAQLREAEDITVFMTTHYMDEAEHVDRIAIMNEGEIATID
                     SPDALKSSIGKDRVQFHTEDDELAITRLRERFGLEAGMHEGAVTVGVAGGAEFVPRLF
                     KELDVPITTVTVTRPSLDDVFMTYTGTTISDAERPKAGAR"
     gene            1716..2618
                     /locus_tag="VH419_06260"
     CDS             1716..2618
                     /locus_tag="VH419_06260"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY7043254.1"
                     /translation="MSLPDQQTSQTSPRDPTHRVVAPRVELAAGGLRHELRATSVVWR
                     REIIRFVQDRARIVAMLLQPLLFLFVMGTGLGSVVSTSEDVDFKTFLFPGVLAMSVLF
                     MAVFAGISLVWDREFGFLREMLVAPISKTAIIVGKCLGGASSATLQACIVLVLGPLVG
                     VPYSVELILTLLVCLFLGSMMLTSLGVVLSARIKTIQAAMPMTQMLIMPMMFLSGALF
                     PLANLPRWLEILTKLNPLTYAVQPMRAAVFNHLDISASTRAQLDPPITWFGWEVPAYV
                     QLGVVAAVTVALIAVAVGLFDRTE"
     gene            2912..3601
                     /gene="ftsE"
                     /locus_tag="VH419_06265"
     CDS             2912..3601
                     /gene="ftsE"
                     /locus_tag="VH419_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020574165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEY7043255.1"
                     /translation="MIRFEKVTKSYAGQNRPALDTVDADIDKGEFVFLVGASGSGKST
                     FLRLILRESSPTSGRVFVAGKEINRLAGWKIPGLRRQIGTVFQDFRLLPNKTVTENVG
                     FALQVIGKPKSVIKRVVPETLELVGLEGKGERMPDELSGGEQQRVAIARAFVNRPMIL
                     IADEPTGNLDPTTSVGIMKLLDRINRTGTTVMMATHDAGIVDQMRKRVIELQSGRVVR
                     DQARGVYGYQS"
     gene            3668..4585
                     /gene="ftsX"
                     /locus_tag="VH419_06270"
     CDS             3668..4585
                     /gene="ftsX"
                     /locus_tag="VH419_06270"
                     /inference="COORDINATES: protein motif:HMM:NF038346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HEY7043256.1"
                     /translation="MQLRYVASELGTGLKRNVSMTIAVILTIWISLTLVGLGLLLRYQ
                     VETIEKYFGSQLEVQVAFCAKNSNSANCVSGQATPQQEQAVLRVIKNSSYVDSYRYRT
                     REEAYQGFQATHTDSEGNPDALSSEIKVSDMPTAYWVKLDDPDDTRALVSSVQNLQGV
                     DQIVDLRDKLAPIYTTLNVLKWLALGGAACLVLAAVLQVSNTIRLAAMARRREIGIMR
                     LVGASSLYIQLPFLLEVVFSALIGAALACLSILVVMQFFVPWLRGELKIWPWVVWGDA
                     SRSMLFIIVIALVLAVVPTLLMTRKYLKV"
     gene            4665..5993
                     /locus_tag="VH419_06275"
     CDS             4665..5993
                     /locus_tag="VH419_06275"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEY7043257.1"
                     /translation="MRSSTVLRFFPAGGAGRARIGVTVALAASLVTAVLAGPSSGAPS
                     TDDTDHSKKLHQAKNRLDHSIDRKKQDLDEISAQLVRAKNRLADAWSDLHDARLEYQR
                     VRAQVKVAADHDREMQALLEQAIVRLEDAQADLVQGTQDVQTERSALAAYAVSSFQTG
                     GTAVLNLGVAFNAETPQQALDGMQASATVLDKQVVALQQYEATKVLLELTEKRVQATK
                     DDVQQKREDAASVLEVKRALQAQARVARANMLDRLGTLQDAKRQIADAKARERRHLQT
                     MQRERNRVEERLRKIAERRARQHQRVAVATTHDGGGYLSYPVMHTYITSPYGMRMHPI
                     LHVWKLHDGTDFHAPCGTAVYAAAPGRVISEYYNVGYGNRLIVDHGFVHGVSLQTSYN
                     HLTSWVAHPGQRVRRGQLIAYSGTTGYSTACHLHFMVYVNGYTVNPVTWL"
     gene            6078..6551
                     /gene="smpB"
                     /locus_tag="VH419_06280"
     CDS             6078..6551
                     /gene="smpB"
                     /locus_tag="VH419_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007077115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEY7043258.1"
                     /translation="MARERGQKLIAQNKKARYDYHIEDTYEAGLVLTGTEVKSLRAGR
                     ASLTDGYAEIIDGEVWLHGAHIPEYLQGTWNNHSPRRKRKLLLNRHEIDKMERRVNER
                     GLTIVPLKMYFKDGRAKVEIALARGKKAYDKRQTIAKRDAQREATRELGRQAKGF"
     gene            6681..>7335
                     /locus_tag="VH419_06285"
     CDS             6681..>7335
                     /locus_tag="VH419_06285"
                     /inference="COORDINATES: protein motif:HMM:NF013360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016641 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, oxygen as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysyl oxidase family protein"
                     /protein_id="HEY7043259.1"
                     /translation="MASTVVIAIALDVVPVAAASDGAAAPPALPRSGVAVSGKRPPAL
                     LPNLRAVQAKDLTIQRVDGRRWLRFTSALANVGPGPLEVRPDNQQRCPRRQRHARQIL
                     YLDVDRTGHYRRAIDTRIRARSAGCMVFHPSHHHWHFNSSARYTIIDPKRHRVVASHP
                     KTSFCLRDSRRVPSRWGVTTRFDAYYGACDRDTKQGISIGWADVYESYLPGQALPLPR
                     "
BASE COUNT         1294 a         2430 c         2468 g         1143 t
ORIGIN      
        1 ccagatggtc aaggacctgc ggaccgagca cgaggtgagc aacccgaccg cggtgctcga
       61 cggggacatc gacgacttca tcgagtccgc catccggtgg cggcggatgg ccgcggccgc
      121 caactgaggc tgtccacacg acgtttgtgc ccacgcccct cgggtacgca aggcgccatg
      181 tcggccatcc acgccacgag cagccaagac ctgctggcgt tgtacctcaa cgaccacctg
      241 ctcggcgcct ccggcggtgt cgagctcttc cgccgtgctg cccgcgggca gtccggcgac
      301 gccgcccgca ctcggctcga ggtgctcacg cgcgacgtcg aacaggaccg cgacgccctc
      361 gtggacctga tgaaacagct cgacgtacga ccagcgcggc accgcgtcgc actgggatgg
      421 ctcggggaga aggtgggacg gctgaagctg aacggcagcc tgctcagccg tacgccgctc
      481 acggacctcg tggagctcga gtaccttcgc ctcggcgtcg aaggcaagct ggccgggtgg
      541 gaggcagtcg aggtggccgc aggcgccgac caccgtatcg accttgcgcg ggtcgaggag
      601 ctacagcagc gcgcgaagga tcagctcgaa gcgctggaag aacttcgccg ggaggcggcc
      661 cgccacgcgt tcgccggtcg aagcgagggc cctcagaagt aactgttctc tgtgccggcg
      721 gccggcgaca taggctgtca agggcggcaa cggcagagag gacgacgtat ggctgagcac
      781 gccatcacgg tcgagggact ggtcaagaac ttcggcgagg tccacgccgt gcgcgggatc
      841 gacttcaccg tctccgaagg cgagatgttc ggcttcctcg ggccgaacgg tgcgggaaag
      901 tcgaccacga tcaagatcct ctgcacgctg gccgatccga cgcaaggcgc agccacggtg
      961 gccgggtacg acgtcgtcac acagcggtcg ctcgtacgcc gctccatcgg actggtcttc
     1021 caggagccga cgctcgacgc gtacctcacc gccgagcaga acctgtcctt ccacggggag
     1081 ctctacggcg taccccgcgc ggccctcaag aagcggatcg acgcagtcct cgagatggtc
     1141 gggctggcgg atcgacgcaa ggacaaggtg cagacgtact ccggagggat gaaacgccgg
     1201 ctcgagatcg gccgtgggct gctccactcc ccgcgggtgc tgttcctgga cgaacccacg
     1261 atcggcctgg acccgcagac gcgcgcgtcc atctgggact acgtggcgca gctgcgcgag
     1321 gccgaggaca tcaccgtgtt catgaccacg cactacatgg acgaggccga gcacgtcgat
     1381 cggatcgcca tcatgaacga gggcgagatc gcgaccatcg actcacccga tgcgctgaag
     1441 tcgagcatcg gcaaggatcg ggtccagttc cacaccgagg acgacgagct cgccatcacg
     1501 cggctgcgtg aacggttcgg tctcgaggcc ggcatgcacg aaggtgcggt gacggtgggt
     1561 gtcgcaggcg gtgcagagtt cgtcccgcgg ttgttcaagg agctggacgt gccgatcacc
     1621 acggtcacgg tcacccggcc gagcctggac gacgtcttca tgacgtacac cggtacgacg
     1681 atctccgacg ccgaacgccc caaggcaggt gcccgatgag tcttcccgac caacagacct
     1741 cgcagacgag cccacgcgat cccacccatc gcgtcgtcgc gccgcgcgtc gagctggcag
     1801 ccggcggtct ccggcacgag ctacgggcca cgtcggtggt gtggcgtcgc gagatcatcc
     1861 ggttcgtcca ggaccgcgct cgcatcgtcg cgatgctgct gcagccgctg ctcttcctgt
     1921 tcgtcatggg gaccggtctc ggcagcgtcg tgtcgaccag cgaggacgtc gacttcaaga
     1981 cgttcctctt tccgggcgtg ctcgcgatgt cggtgctgtt catggcggtg ttcgccggca
     2041 tctcgctggt gtgggaccgc gagttcggct tcttacgcga gatgctcgtt gcaccgatct
     2101 cgaagaccgc gatcatcgtc ggcaagtgcc tcggcggcgc gagctcggcg accttgcagg
     2161 cctgcatcgt gctcgtgctc ggcccactgg tcggcgtccc ctacagcgtc gagctgatcc
     2221 tgacactgct ggtgtgtctg ttcctgggct cgatgatgct gacgtcgctt ggcgtcgttc
     2281 tctccgcgcg catcaagacg atccaggcgg cgatgccgat gacgcagatg ctcatcatgc
     2341 cgatgatgtt cttgtcggga gcgctgttcc cgctcgccaa cctgccgcgc tggctggaga
     2401 tcctcaccaa gctcaacccg ctcacgtacg ccgtccagcc gatgcgggca gccgtcttca
     2461 accacctcga catctccgcg agcacgcgcg cccagctcga cccgccgatc acgtggttcg
     2521 gctgggaggt gccggcgtac gtccagctcg gcgtcgtcgc cgccgtcacc gtcgccctga
     2581 tcgccgttgc ggtggggctc ttcgaccgaa cggagtgagg tcgaagcgag agtgcagcga
     2641 ccgaacggag tgacgggcgt gtcggcagcg gcgaagccgc ggcgacaccc gcgataagcc
     2701 acccgccgtc tcgagagggc aacgccacat gccctagacg gtcgatgtgc ccacgaatgc
     2761 gggaagttcc gcggtttcgg cgccgcaaat cccggaagtc cccgcaattc gcggggacca
     2821 gcggggagca gcggaggcct caggaggttc tcaggctcgc cggtgatggt ggtgccggga
     2881 acgttcaggg gcgtcaccta cactctggcc agtgatccgg ttcgaaaaag tcaccaagtc
     2941 gtacgccggg cagaaccgcc ctgccctcga caccgtcgac gccgacatcg acaaggggga
     3001 gttcgtcttc ctggtcggcg cctccggctc cggtaagtcg acgttcctcc ggctcatcct
     3061 gcgcgagtcg agcccgacgt ctgggcgtgt cttcgtcgcc ggcaaggaga tcaaccggct
     3121 ggccggctgg aagatccccg ggctgcgccg ccagatcggc accgtcttcc aggacttccg
     3181 gctgctgccc aacaagaccg tcaccgagaa cgtcggcttc gcgctgcagg tgatcggcaa
     3241 gccgaagagc gtgatcaagc gcgtcgtacc ggagacgctc gagctggtgg gcctggaggg
     3301 gaagggtgag cggatgccgg acgagctgtc ggggggtgag cagcagcggg tggcgatcgc
     3361 tcgcgccttc gtcaatcgcc cgatgatcct gatcgccgac gagccgaccg ggaacctcga
     3421 cccgacgacg tcggtcggga tcatgaagct gctcgaccgg atcaaccgca ccgggacgac
     3481 ggtgatgatg gccacgcacg acgccgggat cgtcgaccag atgcgcaaaa gggtgatcga
     3541 gctgcagtcc ggacgcgtcg tgcgcgacca ggcccgcggc gtgtacggct accagtcctg
     3601 accacatccg ccggacacac gacatcgccg agaccgacca ggtcggcccg accagaacca
     3661 ggaagccatg cagctgagat atgtcgcctc tgagctcgga accgggctca agcgcaacgt
     3721 gtcgatgacg atcgcggtca tcttgacgat ctggatctcc ctgacgttgg tggggctcgg
     3781 cctcctgctc cgctaccagg tcgagaccat cgagaagtac ttcggcagcc agctcgaggt
     3841 ccaggtggcc ttctgcgcga agaactccaa ctcggcgaac tgcgtgagcg gacaggcgac
     3901 gccgcaacag gagcaggctg tcctccgcgt gatcaagaac agctcgtacg tcgactcgta
     3961 ccgctaccgg actcgcgagg aggcgtacca gggcttccag gcgacacaca ccgactccga
     4021 agggaatcct gacgcgctca gttcggagat caaggtctcc gacatgccga cggcgtactg
     4081 ggtgaagctg gacgaccccg acgacacgcg ggcactcgtc agctcggtgc agaacctgca
     4141 gggcgtggac cagatcgtcg acctacgcga caagctggcg ccgatctaca cgacgctgaa
     4201 cgtcctgaag tggctggcgc tcggcggggc cgcctgcctg gtactcgcgg cggtcctgca
     4261 ggtgtccaac acgatcaggc tcgcagcgat ggcgcgacgg cgagagatcg gcatcatgcg
     4321 gctggtgggc gcatcgagcc tgtacatcca gctgccgttc ctgctcgagg tggtgttctc
     4381 cgccctcatc ggggcagcgc tggcgtgtct gtcgatcctg gtcgtgatgc agttcttcgt
     4441 gccgtggctg cggggtgagc tcaagatctg gccctgggta gtgtggggtg acgcgagcag
     4501 gtccatgctg ttcatcatcg tgatcgcgtt ggtgctggct gtggtcccga cactcttgat
     4561 gacccggaaa tacctgaaag tctgattccg ctccgttacc gtcgaggggc caggttcagc
     4621 agtccgccag ttcagcagtc cgccagttca acggcttcgg atgggtgagg tcttcgactg
     4681 tgctccggtt cttccccgca ggaggcgcag gacgggcccg gatcggcgtg acggtggcgc
     4741 tcgcggcgtc actggtcacc gccgttctgg caggcccgtc gagcggtgcg ccctcgacgg
     4801 acgacaccga ccattccaag aagctccacc aggcgaagaa ccggctcgac cacagcatcg
     4861 atcgcaagaa gcaggacctc gacgagatct cggcgcagct ggtccgtgcg aagaaccggc
     4921 tggcggacgc ctggtcggat ctgcacgatg cgcggctgga gtaccagcgc gtgcgcgcac
     4981 aggtgaaagt ggccgccgat cacgaccgcg agatgcaggc gctgctggag caggcgatcg
     5041 tccggctcga ggacgcgcag gcggacctgg tgcaggggac gcaggacgtg cagaccgagc
     5101 gttcggcgct ggcggcgtac gccgtctcca gcttccagac cggcggcacc gcagtgctca
     5161 acctgggtgt cgccttcaac gccgagacgc cgcagcaggc gctcgacggc atgcaggcgt
     5221 cggcgacggt gctggacaag caggtggtgg cgctgcagca gtacgaagcg acgaaggtgc
     5281 tgctcgagct cacggagaag cgggtgcagg ccaccaagga cgacgtccag caaaagcgcg
     5341 aggacgccgc cagcgtgctc gaggtcaagc gtgctctcca ggcgcaggcc cgggtcgcga
     5401 gggcgaacat gcttgaccgg ctcggcacgc tccaggacgc caagcggcag atcgccgacg
     5461 cgaaggcacg tgagcgtcga cacctgcaga cgatgcagcg tgagcgtaac cgggtcgagg
     5521 agcggctgcg caagatcgcc gaacgcaggg cacgacagca tcagcgcgtc gccgtcgcca
     5581 cgacccacga cggcggcggc tatctcagct acccggtgat gcacacctac atcacgtcgc
     5641 cgtacgggat gcggatgcat ccgatcctgc acgtgtggaa gctccacgac ggcaccgact
     5701 tccacgcgcc gtgtgggact gcggtgtacg cggcggcgcc tggccgggtc atctcggagt
     5761 actacaacgt gggatacggg aaccggctca tcgtcgacca cggtttcgtg cacggcgtga
     5821 gcctgcagac gtcgtacaac cacctcacgt cctgggtggc gcacccgggc cagcgggtgc
     5881 ggcgtggcca gctgatcgcg tactccggga cgaccggcta ctcgactgcc tgccacctgc
     5941 acttcatggt gtacgtcaac ggctacaccg tgaaccccgt cacctggctc tagcgtggcc
     6001 cggcttgcgt ccgtgcgccg caacgtcctg ggagccgccg ccacggacgc aaactcgggt
     6061 aggcgagaat ggggctcatg gccagggagc gcgggcagaa gctgatcgcc cagaacaaga
     6121 aggcgcgcta cgactaccac atcgaggaca cctacgaggc cgggttggtc ctcaccggaa
     6181 ccgaggtgaa gtcgctgcgg gccggtcgcg cgtccctgac cgacgggtat gccgagatca
     6241 tcgacggcga agtgtggctg cacggcgcac acataccgga gtacctacaa ggcacgtgga
     6301 acaaccacag tccgcggcgc aagcgcaagc tgctgctcaa ccggcacgag atcgacaaga
     6361 tggagcgtcg ggtcaacgaa cgcggcctga cgatcgtgcc gctgaagatg tacttcaagg
     6421 acggccgcgc gaaggtcgag atcgcgctgg cccgcggcaa gaaggcctac gacaagcggc
     6481 agaccattgc caagcgcgac gcccagcgcg aggcgacgcg cgagctcggc cgccaggcca
     6541 agggcttctg aacgccgtcg ctgctgttag gtttggccgg tcgcgcccac gatcaggtca
     6601 ggagtcccat ggcgatctcg cggtatcacc tcggtcggtc agtggctcgc cgctcgcctt
     6661 cttgcgcgat attcgctgcg gtggcgtcaa ccgttgtcat cgcgatcgcc ctggacgtcg
     6721 taccggtcgc tgctgcgtcc gacggcgctg ccgcaccacc ggcgctgccg cgctccggcg
     6781 tcgccgtgtc cggcaagagg ccgccggcgc tgctgccgaa cctcagggcc gtgcaagcga
     6841 aggacctcac catccagcgc gtcgacggaa ggcgatggct gcgattcacc tctgcgctgg
     6901 cgaacgtcgg cccgggcccg ctggaggtgc gccccgacaa ccagcaacgg tgtcctcgca
     6961 ggcagcggca tgcccggcag atcctctacc tcgacgtcga ccgcaccggt cactacagac
     7021 gcgcaatcga cacgaggatc cgcgcgcgat cggccggctg catggtcttc cacccgagcc
     7081 accaccactg gcacttcaac tcctccgccc gctacacgat catcgacccg aagcgccatc
     7141 gcgtcgtggc gtcgcatccg aagacgagct tctgcctgcg cgacagccgc cgggtcccga
     7201 gccggtgggg tgtgacgacg aggttcgacg cgtactacgg cgcgtgtgac cgcgacacga
     7261 aacagggcat ctcgatcgga tgggctgacg tctacgagtc gtacctgccc ggtcaggcac
     7321 tgccgttgcc gcgtc
//
LOCUS       DATBFZ010000211         4249 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_4993934, whole genome shotgun sequence.
ACCESSION   DATBFZ010000211 DATBFZ010000000
VERSION     DATBFZ010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 4249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4249
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4993934"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="VH419_06290"
     CDS             <1..256
                     /locus_tag="VH419_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043260.1"
                     /translation="VRLQALITAIRDVGFLVVGFGGVIYQQLTGKVNPWLLAVFTTML
                     GIPGAIGLLQLRGGSGKQTVPTTPSPPSSSPSSSSPSPPQ"
     gene            281..580
                     /locus_tag="VH419_06295"
     CDS             281..580
                     /locus_tag="VH419_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043261.1"
                     /translation="MPLTHPVSRSTWYILAVVYASMFLAVLGNMLYTNHVAEQSVQRS
                     RQQAELNDQRWCGLLEKYREAYSSNPAPTTQLGRDIRAQLEQLYEDFRCASVSKP"
     gene            599..802
                     /locus_tag="VH419_06300"
     CDS             599..802
                     /locus_tag="VH419_06300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043262.1"
                     /translation="MWTKKFWQETAEWVATAAAAAVLTAWQSGLNDWGDVGQAVAIAA
                     GTALLKCLLASQKGDPTSASLTR"
     gene            799..1023
                     /locus_tag="VH419_06305"
     CDS             799..1023
                     /locus_tag="VH419_06305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043263.1"
                     /translation="MKLTTPCEQGACLSLETPCDTNACICLTGPPNSLVLTSNQSTGS
                     VVVTADEVRQFAAAVQAGFFDELLGGGLDG"
     gene            complement(1101..1328)
                     /locus_tag="VH419_06310"
     CDS             complement(1101..1328)
                     /locus_tag="VH419_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043264.1"
                     /translation="MAAVVEIPPEHCSTCPETRLRPGWDECPLPGCRKMDRSYRCPAN
                     HLTMATWHEHRIVPSERPRWHCAYEDLTSPS"
     gene            complement(1616..2014)
                     /locus_tag="VH419_06315"
     CDS             complement(1616..2014)
                     /locus_tag="VH419_06315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043265.1"
                     /translation="MREDEDVSQTEPSEIFKASRDPQRHPRTDDGKFVRPSTCPDCGV
                     HVGLPHEQGCDVARCPECGTQRIQCDEPGHDIDDDRAVWTGLWPGLAECRRLGWYVGG
                     REDLNRLAFAGATGELSWDSTRQEFVQPGE"
     gene            complement(2011..2247)
                     /locus_tag="VH419_06320"
     CDS             complement(2011..2247)
                     /locus_tag="VH419_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043266.1"
                     /translation="MNDIRFAAKVENAADSLDPAQIRTMAVTLIAEAGDNVLAQTRRA
                     LEEAYADNDPDLYALWEAIQVEVLNGGPSAEVYA"
     gene            complement(2258..2707)
                     /locus_tag="VH419_06325"
     CDS             complement(2258..2707)
                     /locus_tag="VH419_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043267.1"
                     /translation="MNHEIQPGHDHPNYVRCGHNCPDVIAREQGEMRTIDEFLADPTP
                     AEQPELTVKPLLVDDFPVDYDEGSINIYVRDGDVNALLSVTDRPRDYEPDEEDEDDVT
                     TGVDMFLDDDELMEIAKTCARVLAAMGKRDHLAELISEAATALAENR"
     gene            3094..3300
                     /locus_tag="VH419_06330"
     CDS             3094..3300
                     /locus_tag="VH419_06330"
                     /inference="COORDINATES: protein motif:HMM:NF019884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043268.1"
                     /translation="MLRHTLNGRQVLTIEDLALRYEVTEEAIRQTIARAAERGKPIPI
                     AGKCGRKALYDPKVFDRARGVTPG"
     gene            complement(3287..3556)
                     /locus_tag="VH419_06335"
     CDS             complement(3287..3556)
                     /locus_tag="VH419_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043269.1"
                     /translation="MLTEPELTQLAHDVLDLIYQRTTPRLSADPDETETLTAALEVAA
                     ALKKTLPKVSKLLDQKARDAGMMLAAIGLASGTRKQSVHRRVSRG"
     gene            complement(3599..3835)
                     /locus_tag="VH419_06340"
     CDS             complement(3599..3835)
                     /locus_tag="VH419_06340"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY7043270.1"
                     /translation="MPTTVPKYEKVMNDLRRRIAEGEFEPTGRLPSTRQLVDHYGFSR
                     ETIRQAITRMQMLGELQGEQGDAVYVVKKDEAAE"
     gene            4039..>4249
                     /locus_tag="VH419_06345"
     CDS             4039..>4249
                     /locus_tag="VH419_06345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043271.1"
                     /translation="MESRDLHFDLAPDEIALREAISHGGGERQLDPLARALHDLRRPR
                     ELTRDEWNAAYLAIQEHNIRRLGPSV"
BASE COUNT          644 a         1403 c         1453 g          749 t
ORIGIN      
        1 cgtgcgactt caggcactga taacagcgat acgggacgtc gggttcctgg tggtgggctt
       61 cggcggagtg atctatcagc aactcaccgg aaaggtgaac ccgtggctgc tggccgtgtt
      121 cacgaccatg ctcggcatcc cgggagccat cggactgttg cagctacgtg gtggcagtgg
      181 caagcagacc gtccccacta ctccctcgcc gccatcgtcg tctccgtcgt cctcctcgcc
      241 ctcgccgccg cagtgacgat cgcagcccgg gggtggtgag gtgccgttga cgcatccggt
      301 atcgcggtcg acctggtaca tcctggccgt cgtgtacgcg tcgatgttcc tggcggtgct
      361 cgggaacatg ctctacacca accacgtcgc cgagcagagc gtgcagcgtt cccgccagca
      421 ggcggagctg aacgaccagc gctggtgcgg gctgctggag aagtaccgcg aggcgtactc
      481 gtccaacccg gcgccgacga cgcagctcgg ccgggacatc cgcgcgcagc tggagcagct
      541 gtacgaagac ttccgctgcg catccgtcag caagccctga cgagccaagg aggctcagat
      601 gtggaccaag aagttctggc aggagactgc cgagtgggtt gccaccgcgg cggcggcggc
      661 ggtgctgacc gcctggcagt cgggtctcaa cgactggggc gacgtcggcc aggctgtggc
      721 gatcgcggcc ggtacggcgc tgctgaagtg cctgctcgcg tcgcagaagg gtgacccgac
      781 gtcggcgagc ctgacgcggt gaagctgacg accccatgcg agcagggcgc ctgcctgtcg
      841 ctcgaaacgc cgtgcgacac caacgcctgc atctgcctga ccggaccgcc gaacagtctg
      901 gtgctgacat cgaaccagtc cactggttcg gtcgtggtga cggccgacga ggtgcgccag
      961 ttcgcggcgg cggtgcaggc tggcttcttc gacgagctac tgggtggtgg tctcgatggc
     1021 tgacgagctg ccgacggccg aggagctgga cgagatcatc ctgcggatac tcgaagccga
     1081 gtcggtcgag gtcgccgcgt tcaactcggc gatgtgaggt cctcgtacgc gcagtgccac
     1141 ctcggccgct ccgacggcac gatgcggtgc tcgtgccagg tggccatcgt gaggtggttg
     1201 gccgggcacc ggtagctgcg gtccatcttg cggcagccgg gcagcgggca ttcgtcccag
     1261 ccggggcgta ggcgggtttc cgggcacgtt gagcagtgtt cgggtgggat ctcgacgacg
     1321 gccgccatgc cccggagcgt acatccgttc gaaccgtggc gaaaagattc ggtcgccgtg
     1381 agcagatgat tccgtcattc cgtcatgggt cggcgtaggc tcatcttggc ctacgaccag
     1441 ggttgctcta tccgagctac tgtgcgcggc ggtcaacggc tccggctggt gtaacccgcg
     1501 cactcacctt ctaccctgcc aggttccggg gatcggctcc cggggttggt ctgagccgcc
     1561 atcagacacg aacggcccgc cccagttggg gcgggccgtt tcgtcatgtc cgggctcact
     1621 cgcctggctg cacgaactcc tgcctcgtgc tgtcccagga aagctcgccg gtcgccccgg
     1681 cgaaggccag gcggttcagg tcctcgcggc cgccgacgta ccagccaagg cggcggcact
     1741 cggccaagcc cggccacagg cccgtccaga ccgcgcggtc gtcgtcgatg tcgtggcccg
     1801 gctcatcgca ctggatccgc tgcgtgccgc actcgggaca ccgggcgacg tcgcatccct
     1861 gctcgtgcgg cagcccaacg tggacgccgc agtcggggca ggtcgacggc cgcacgaact
     1921 tgccgtcatc ggtacgcggg tgccgctgcg gatcgcggga tgccttgaag atctcgctgg
     1981 gttcggtctg gctcacgtcc tcatcctctc tcacgcgtac acctcggcgg acggcccgcc
     2041 gttgagtacc tcgacctgga tcgcctccca cagcgcgtac aggtccgggt cgttgtccgc
     2101 gtacgcctcc tccagcgcac ggcgggtctg cgccagcacg ttgtcgccgg cctcggcgat
     2161 cagcgtcacg gccatggtgc ggatctgcgc cgggtcgagg gagtcggcgg cgttctcgac
     2221 cttggcggcg aagcggatgt cgttcatggt ggcctcctca gcggttctcg gcgagcgccg
     2281 tggcggcctc gctgatcagt tcggcgaggt ggtcgcgctt gcccatcgcg gcgagcacgc
     2341 gggcgcaggt cttggcgatc tccatcagct cgtcgtcgtc gaggaacatg tcgacgccgg
     2401 tggtcacgtc gtcctcgtct tcctcgtcgg gctcgtagtc gcgcgggcgg tcggtcacgg
     2461 atagcagggc gttgacgtcg ccatcgcgga cgtagatgtt gatcgagcct tcgtcgtagt
     2521 cgacggggaa gtcgtcgacg agcagcggct tgacggtcag ctcgggctgc tcggcggggg
     2581 tcgggtcggc gaggaactcg tcgatggtgc gcatctcgcc ctgctcgcgg gcgatcacgt
     2641 cggggcagtt gtggccgcag cgcacgtagt tcgggtggtc gtgtccgggc tggatctcgt
     2701 ggttcatggc ggttcctttc ggggtgaggt gttgtgctct ctagagtgtc acgcaatgcg
     2761 ttacaccacc acgagccctg acctgccccg atccggcaga aggagacgcg gggtgcgctg
     2821 gccgtccgac acccgtacta aatcgttccg actatcacgc tcggcaaccg tccaatgggg
     2881 actcggtaag gccggagctg tcacgcaacc cgttacactc gtccgatcgg tcaagaagtc
     2941 tgtactcaga ggatgagagc ttgcgaccgg ctctgtccgg attcgccgga agccgcgccg
     3001 aaactcgtgg aacagaacgc cctaaccgga cgaccgcagg ccgggctatc cgtcacgtaa
     3061 cgtaatccgt gaccgctctg gtacgctcac cgcatgctcc ggcacaccct caacggccgc
     3121 caggtcctca ccatcgagga tctggctctg cgctacgagg tcaccgaaga agccattcgg
     3181 cagaccatcg cccgggccgc cgaaagaggc aagccgatcc ccatcgcggg caagtgcggg
     3241 cgcaaagccc tctacgaccc gaaggtcttc gaccgggccc ggggcgtcac ccccggctga
     3301 cccgccgatg caccgactgc ttacgcgtac cgctcgccag cccgatcgcc gccagcatca
     3361 tgccggcgtc gcgcgccttc tggtcgagca gcttcgacac cttcggcagg gtcttcttca
     3421 gcgccgcggc gacctccagg gcggcggtca gcgtctcggt ctcgtccggg tcagcggaca
     3481 ggcgcggggt ggtgcgctgg tagatgaggt ccaggacgtc atgggcgagc tgggtcagct
     3541 cgggttcggt cagcacgatg agacctcccg tggtgggaag tggtgggcgg gcgctcgact
     3601 actcggcggc ctcgtccttc ttgacgacgt acacggcgtc gccctgctcg ccctggagtt
     3661 cgccgagcat ctgcatccgc gtgatggcct ggcggatggt ctcgcggctg aacccgtagt
     3721 ggtcgacgag ctgacgcgtg gaaggcaggc gaccggtcgg ctcgaactcg ccctcggcga
     3781 tccggcgacg caggtcgttc atgaccttct cgtacttggg cacggtcgta ggcatgatga
     3841 cactcccagc tagacctgct gatgtcatca cacctcctgt gatctttgca agcattgccg
     3901 gtacggttgg actatacatg taaagcatgt aaggtggcga cgaaggccgc tcccagctag
     3961 acactgtgcg gcggcctgac gccgggcggt cctacgagct ccggaccgcc cggcccttcc
     4021 gaccggggag gtagaccctt ggaatcccgc gacctgcact tcgacctggc gcccgacgag
     4081 atcgcgctgc gcgaggcgat cagccacggc ggcggggagc gtcagctcga cccgctcgcc
     4141 cgcgcgctgc acgatctgcg ccgtccacgt gagctgaccc gcgacgagtg gaacgcggcg
     4201 tacctcgcga tccaggagca caacatccgg cggctcggcc catcggtgg
//
LOCUS       DATBFZ010000212         8698 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_468792, whole genome shotgun sequence.
ACCESSION   DATBFZ010000212 DATBFZ010000000
VERSION     DATBFZ010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 8698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8698
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468792"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="VH419_06350"
     CDS             complement(<1..439)
                     /locus_tag="VH419_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011753726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase"
                     /protein_id="HEY7043272.1"
                     /translation="MTPSTQALDEIKIGGMGASRKRVEDNRFIRGRGTYIDDIVLPGM
                     LHMEMLRSPLAHARIRSIDTSKAWDLPGVRLVLTGEMMATRNLAWMPTLSYDTQAVLA
                     TDKVRFQGQEVACVVADDPYIAKDACEAIVVEYEPLPVIVNPTQ"
     gene            complement(444..1025)
                     /locus_tag="VH419_06355"
     CDS             complement(444..1025)
                     /locus_tag="VH419_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011753725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEY7043273.1"
                     /translation="MTSLFAEITSEPAADVPVRRVTLTVNGVRRTAEIEPRLLLAHLL
                     RQGLGLTGTHTGCDTTNCGACTVLLDGEPVKSCTMLGVQADGHEVTTVEGLAGASELH
                     PLQEGFKGEHGLQCGFCTPGMMLTAKALLEENPDPTEDEVRWALSGNLCRCTGYQNIV
                     KSVLWAAAKMRGTADTEGPDEADSTGTAASEES"
     gene            complement(1029..1904)
                     /locus_tag="VH419_06360"
     CDS             complement(1029..1904)
                     /locus_tag="VH419_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011753724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEY7043274.1"
                     /translation="MYPSRFRYDAPRSIDEAIALLSDGDGEAKVLAGGQSLVPLMKLR
                     FASPELVVDINNLPALDYHRTDTDGTTRIGALCRHATLERSTLLAERQPTMSAAAPLI
                     ADPIVRRRGTLVGSLCHADPQGDWAAVVTALGGHVVAQGPNGQRTIPIGEFVTGPFQT
                     ALGYDEIAVEAVIPAATATRMGGYLKLERRVGDFATAGVAVALEMSGDQVTRAGIGLT
                     GVGPATIDVTEAAEALAGRALTADRIDEAAGLAAQAAQPRSDHRGTADYKRHIVHTFV
                     TRLLTRVADDTERAA"
     gene            complement(1926..2201)
                     /locus_tag="VH419_06365"
     CDS             complement(1926..2201)
                     /locus_tag="VH419_06365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043275.1"
                     /translation="MRVESSGHSRGNPRLYELIENAPKTGKSPTVWAWPELVDHLKVA
                     GQPVQGPWPPHQRPRPDPTDVEALPVAVPDPEADLPRHLDSSDPAEA"
     gene            2489..3487
                     /locus_tag="VH419_06370"
     CDS             2489..3487
                     /locus_tag="VH419_06370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEY7043276.1"
                     /translation="MRRLTSVLGTIVLVGMVSALNPTSAPAADRHGRLQDRIDLPDGW
                     QPEGITSHGKTLFTGSLANGAIWEADARTGEGEILSPGAAGSVAVGLDYDRRRDLIWA
                     AGGGTNVVRVYDGDDGHVVATYDFPSDTPRFLNDLVVTRRAVYATDSFNQELCVIPLG
                     RRHGGHGHGDVQNNGRGDHGHGRHLPPPSAATTLPLTGDVVFEDGFNLNGIASDRRLL
                     ITVQSNTGQLFRIDPRTGETFEIDLGGDLVTNGDGLELDGRTAYVVRNQNNLIAVVDL
                     DRHGDSGEVVDELTAAGLDVPATATLVGGSLFAANARFTTPPTPTTPYWITRVAVD"
     gene            complement(3513..4334)
                     /locus_tag="VH419_06375"
     CDS             complement(3513..4334)
                     /locus_tag="VH419_06375"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY7043277.1"
                     /translation="MDDEALATKRQWGQIFDRAEPTYDQFASSYFSHFGPSLVKRAAP
                     VAGSKVLDVACGKGAVLLAAMEQMGGAGLGLGVDISPGMARSLQRLARERKFPNVSSA
                     VMDAENLGVADAAVDVVLSAFSLHFLPEPERTAAEFRRVLRPGGTLGISEWGDADPRW
                     AWEGDLLAAAGSAGPYVARHFEKVDDVKILLEGAGFSSLDADSEEWTVHFADVDEWWA
                     WKWSFGFRRHLDAMSEEGRRRFKHAAEEAMRPLATSQGYPMTLHANFVVGRAGHG"
     gene            5182..5481
                     /locus_tag="VH419_06380"
     CDS             5182..5481
                     /locus_tag="VH419_06380"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEY7043278.1"
                     /translation="MFVRIWRYDVADDQQAEFERVYGPDGDWARLFAASDGFMGTELY
                     VRLGETGRYLTVDRFTSLAAWESFRAEQSRSYAELDLATERLRLTEDELGTGDDG"
     gene            5478..5975
                     /locus_tag="VH419_06385"
     CDS             5478..5975
                     /locus_tag="VH419_06385"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEY7043279.1"
                     /translation="MTSPRVLTAEECVRLLGREGVARVAICTPVGPRIFPVNYVCEGG
                     DSIVFRTSPSSTLGTFAWGTEVAFQVDELDWTTSSGWSVEAVGRPVVVHDPVEIAELR
                     GHGEPHPWAGGSREMFIRLRWKEIAGRSVGTAPLPQEPRDLRPADEDHSALSWHDRRK
                     DHEGP"
     gene            5972..6412
                     /locus_tag="VH419_06390"
     CDS             5972..6412
                     /locus_tag="VH419_06390"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEY7043280.1"
                     /translation="MSDTGPGNGWPPRRLEELARAECVRLLGSKSVGRVAFVAEGSPV
                     VLPVNYVSSDGMVYFRTSTHNVMAAHLLDAAAAFQVDDVDEFMESGWSVLVQGWAEFL
                     DPEVETEYGLERLAHRIEPWAAGARYLLVRIKPERISGRRLEPR"
     gene            6498..7070
                     /locus_tag="VH419_06395"
     CDS             6498..7070
                     /locus_tag="VH419_06395"
                     /inference="COORDINATES: protein motif:HMM:NF039480.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6529 family protein"
                     /protein_id="HEY7043281.1"
                     /translation="MAAQVQESRTPARFAYVALGVGAVVAVVLGVFGKAAHDEGHAAV
                     LTTGFGGLATMKVYLSTVVAVLALVQIVTALWLYGRLGSRIPRHLGVVHKTSGALAVL
                     LSIPVAFNCLWLLGFESYNFRVAMHSIAGCVFYGAFVAKVMGLHARRAPGWLVTVAAG
                     LVFAAIVVVVWTGAVWYVGEFGWPSTSTGE"
     gene            complement(7097..7462)
                     /locus_tag="VH419_06400"
     CDS             complement(7097..7462)
                     /locus_tag="VH419_06400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043282.1"
                     /translation="MTEEHPIAYTALQRGTQVLSSDGDVIGTVERVLDIPDLDLFDGI
                     VVETPGGDRFVDRDQIDRITNLAVHCALTTSEAATLPLPDSAPTYRADPRRMRGTSMM
                     ARLKRRFGRATWNRDHDDE"
     gene            complement(7566..7976)
                     /locus_tag="VH419_06405"
     CDS             complement(7566..7976)
                     /locus_tag="VH419_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017934235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HEY7043283.1"
                     /translation="MVLADDFGDGDVFLSILWLTLFFIWIWLLIVVFGDIFRSDDLSG
                     WGKALWIIFIIILPYLGVLVYLIARGHKMSARAMQAAQQQDAATREYIRSVAGSGGGG
                     GGGTADEIARLADLRDKGVITEEEFQSGKSKALS"
     gene            8250..>8698
                     /locus_tag="VH419_06410"
     CDS             8250..>8698
                     /locus_tag="VH419_06410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043284.1"
                     /translation="MAVASPEMWQSIRGAGVGSPVGVPSTNASTTGAGSPARRSSKAA
                     VRPRVWNSSSQPGAIQHTASRTIAARSERVGHGGRGMRDVAPVNQQLAQQDPQGKGHR
                     NLSPSTATFTGVPAHPTGGKRRRGSNRLACSGKDASDVGVGEEVDRR"
BASE COUNT         1397 a         2920 c         2950 g         1431 t
ORIGIN      
        1 cctgggtcgg gttcacgatc accgggagcg gctcgtactc gacgacgatc gcctcgcacg
       61 cgtccttcgc gatgtacggg tcgtcggcga cgacacaggc gacctcctgc ccctggaagc
      121 gcaccttgtc ggtcgccagc actgcctgcg tgtcatagct gagtgtcggc atccacgcga
      181 ggttgcgggt cgccatcatc tctccggtca gcacgaggcg tacgccggga aggtcccacg
      241 ccttgctcgt gtcgatcgac ctgatgcgcg cgtgcgctaa cggactccgc agcatctcca
      301 tgtgcagcat gccgggcagc acgatgtcgt cgatgtaggt gccccggccg cggatgaacc
      361 tgttgtcctc cacccgtttg cggcttgcgc ccatcccgcc gatcttgatc tcgtcgagcg
      421 cctgcgtcga aggtgtcatc gcgtcagctc tcctcgctcg ccgcagtgcc ggtggagtcg
      481 gcctcgtcgg gaccttcggt gtcggcggtg ccgcgcatct tcgcggccgc ccagagcacc
      541 gacttcacga tgttctggta gccggtacag cgacacaggt tgcccgacag cgcccagcgg
      601 acctcgtcct cggtgggatc cgggttctcc tcgagcagcg ccttggcggt gagcatcatc
      661 ccgggtgtgc agaagccgca ctgcagaccg tgctcgccct tgaagccctc ctgcaacggg
      721 tgcagctcac tggcgcccgc gaggccctcg accgtcgtca cctcgtggcc gtccgcctgg
      781 acacccagca tggtgcagct cttgacgggt tcaccgtcca acagcaccgt gcacgcgccg
      841 cagttggtgg tgtcgcagcc cgtgtgcgtc cccgtgaggc ccagcccttg ccggagcagg
      901 tgggcaagga gcagtcgtgg ttcgatctcg gcggtacgcc gcacgccgtt gaccgtgagg
      961 gtcacgcggc gtacagggac gtccgccgcc ggctcactgg tgatctctgc gaacagactc
     1021 gtcatcgctc aggcagccct ttcggtgtcg tctgcgaccc gggtgaggag gcgggtgacg
     1081 aacgtgtgga cgatgtgccg cttgtagtcc gcggtgccgc ggtggtccga tcgaggctga
     1141 gccgcctgcg cggccaggcc ggccgcctca tcgatgcggt ctgcggtcag cgcgcgcccg
     1201 gcgagggctt ccgccgcctc ggtgacgtcg atcgtcgcgg gtccgacgcc ggtgagaccg
     1261 atccccgccc gcgtcacctg gtcgccggac atctcgaggg cgaccgccac acctgccgtt
     1321 gcgaagtcgc cgacgcgccg ctcgagcttg aggtagccac ccatccgggt cgccgtcgcc
     1381 gcggggatga ccgcctcgac cgcgatctcg tcgtacccga gtgctgtctg gaacgggccg
     1441 gtgacgaact ccccgatggg tatcgtccgc tgcccgttcg ggccttgtgc gacgacgtgg
     1501 ccaccgaggg cggtcacgac cgccgcccag tcgccctgcg gatcagcgtg acacagggaa
     1561 ccaacgaggg ttccacgtcg gcgcacgatc gggtcggcga tcagtggagc cgccgcggac
     1621 atggtcggct gccgttcggc gaggagcgtc gaccgctcca gcgtcgcgtg ccgacacagc
     1681 gcgccgatcc gtgtggtccc gtcggtgtcg gtgcggtggt agtcgagggc cggcaggttg
     1741 ttgatgtcga cgacgagctc cggcgatgcg aagcgcagct tcatcagcgg caccaggctc
     1801 tgtccgcccg cgagcacctt ggcctcgccg tccccgtcac tcaacagggc gatcgcctcg
     1861 tcgatcgagc gcggcgcgtc gtaccggaat cgtgacgggt acatgccgac ctcctcgggc
     1921 ggccgtcagg cttcggcggg gtcggaggag tccaggtgcc gagggaggtc ggcctccggg
     1981 tcgggtacgg cgacaggcag tgcctcgacg tcggtcggat cgggacgcgg gcgctggtgc
     2041 ggtggccagg gtccctgcac gggctgtccg gcgaccttga ggtggtcgac gagctcaggc
     2101 caggcccaga ccgtcgggct cttcccggtc ttcggtgcat tctcgatcag ttcgtagagc
     2161 cgcgggttcc ccctgctgtg cccactcgat tccacacgca tcgctgggtc cttcctgtac
     2221 ggacgcgccg gacccccgtc atgcaccgat ctgcgccctc atcttccaac gagcgcgatc
     2281 aacaggcaag catttgaccg gtcaatcatc cttgacgagg tcaacgtgcc gaaggccgtg
     2341 caggttcagc gatccgcttc ggtggggatg ccgccccagc agccacgaag cgaccgactg
     2401 ggtgcccttt cgtacagttg agacgcacgt gtgcacggca cacgatgagg gaacacctga
     2461 cgggtccgca cacccacgag gagttccgat gcgacgcctc acctccgtac tcggcaccat
     2521 tgtcctggtc gggatggtca gtgccctgaa ccccacttcc gcacccgcgg cggacaggca
     2581 cggacggctg caagaccgga tcgacctgcc cgacggctgg cagccggaag gcatcacctc
     2641 acacgggaag acgctcttca ccggatcgct cgcgaacgga gcgatctggg aggcggatgc
     2701 gcggaccggc gaaggcgaga tcctctcgcc aggagccgcg ggcagcgtcg cggttggcct
     2761 cgactacgac cgccggcgtg acttgatctg ggccgctggt ggcggcacca acgtcgtgcg
     2821 cgtctatgac ggcgacgacg gacacgtcgt ggcgacgtac gacttcccgt cggacacgcc
     2881 acgattcctc aacgacctcg tcgtcacccg tcgcgccgtg tatgcgacag actccttcaa
     2941 ccaggagctg tgcgtgatcc cactggggcg caggcatggc gggcacggac acggcgacgt
     3001 ccagaacaac ggtcggggcg accacggtca cggccgacac ctgccacctc catcggcggc
     3061 gacgacgctc cctctcaccg gtgacgtcgt cttcgaggac gggttcaacc tcaacgggat
     3121 cgcgagtgac cgcaggctgc tgatcacggt ccaaagcaac accgggcagc tcttccgcat
     3181 cgatccgcgc accggcgaga ccttcgagat cgacctcggt ggtgacctgg tgaccaatgg
     3241 cgacgggctc gagctggacg gccggaccgc gtatgtcgtc cgcaaccaga acaacctgat
     3301 cgccgtcgtt gacctggacc ggcacggtga ctcgggcgag gtcgtggacg agctcaccgc
     3361 cgctgggctc gacgtcccgg caacggcgac gttggtcggc ggatccctgt tcgcggccaa
     3421 cgcgcggttc accacaccac ccacgccgac gacgccgtac tggatcaccc gcgtcgccgt
     3481 cgactgaggt gctgcgcgtc tgcttgaccc gctcagccgt gtcctgctcg gccgacgacg
     3541 aagttggcgt gcagggtcat cgggtagccc tggctcgtcg ccagcggccg catcgcctcc
     3601 tcggcagcgt gcttgaacct gcggcggcct tcttcgctca tggcgtcgag atgacgacgg
     3661 aacccgaacg accacttcca ggcccaccat tcgtcgacat cggcgaagtg gaccgtccac
     3721 tcttcggagt cggcgtccag gctcgagaaa cctgcaccct cgaggaggat cttgacgtcg
     3781 tcaaccttct cgaagtgccg ggcgacgtac ggtccggcgg agccggcggc ggcgaggagg
     3841 tccccttccc atgcccatcg cgggtcggcg tcaccccact cgctgatgcc cagggtgccg
     3901 cctggtcgga gtacccgcct gaactcggcc gcggtgcgct cgggttcggg aaggaagtga
     3961 agggagaacg cggagagcac gacgtcgacg gctgcatcgg cgacgccgag gttttccgca
     4021 tccatgaccg cggacgagac gttggggaac ttccgctccc gcgccagccg ctgcagagag
     4081 cgggccatcc cgggggagat gtcgaccccc agaccaagac cggcgccgcc catctgctcc
     4141 atcgctgcga gcaggaccgc gcccttgccg caggcgacat ccagcacctt gcttccggca
     4201 accggtgcgg cccgtttcac cagactgggt ccgaagtgag agaagtacga cgatgcgaac
     4261 tggtcgtacg tcggttccgc ccggtcgaag atctgacccc attgacgttt ggtggcaagc
     4321 gcctcgtcgt ccatggcagc agggtacgtg cggacgctcg gcgtggtggt ttgcgatgtt
     4381 tacagcccaa acggcgtgta aacatcgcaa acctcggatg gacacagcgt gtctcggatg
     4441 tggacatcgc aaacaacagc cagctcagcg accgaagtcc gaaaaccgcc agtcacacac
     4501 caggcacaag gaacgtaacg tcggccgacc agctcagcga gcacgaacgc ttcccgctcc
     4561 tcgctgccaa tccacctcag ccgagtgccg acggtgcgcc ggacgacctc gcccggccag
     4621 tcgcgaccat ctccgccacg gtcgcctccg ataaagccac cttcaccacc aaacggacag
     4681 gtgtcggtgg tggtttgcct cggtagccat gcaaacggac ggctgaccag gcagatctgc
     4741 cttgcgaggg gtgggtctgc ctggggagcc atgcaatccg ccgctcgtca accggcggat
     4801 ctccgagaga ccgtcagtca gacgcctgcc aaaggaggcg gaaagcggcc ggccagctcc
     4861 gcggggcagc aactttggcc gctcttcgct gccgatccac ctcagcccat gtcgacagtg
     4921 cgcctggcga ccacctccct ccgccgcgac catctccgcc accgtcgcct ccgaaaaaag
     4981 ccaccttcac caccaatcgg acacgcgggc gcggtgcttt gcgatgtcga catcgcagac
     5041 ctgcgctgtg aacccgagat ctgcgatgca aagacaggac ctgggctgta aacatcgcaa
     5101 acccaccagg aacctcggcg aagagacggt ggagtgccgg gcgggcttca cagccggttg
     5161 ggttgcggtc acgatggcat ggtgttcgtg cggatctggc ggtacgacgt ggccgacgat
     5221 cagcaggcgg agttcgaacg tgtctacggt ccggacggcg actgggctcg actgttcgcc
     5281 gcctcggacg ggttcatggg caccgagctg tatgtgcgac tgggcgagac tggtcggtac
     5341 ctgaccgtcg accggttcac cagcctggcc gcgtgggaga gcttccgtgc cgagcagagc
     5401 aggtcgtacg ccgagctgga cctggcaacc gagcgactgc ggctcaccga agacgagctc
     5461 ggcacgggtg atgacggatg acgagcccgc gtgtcttgac agcggaggag tgcgtacggc
     5521 tccttggacg tgagggcgtg gccagggtgg caatctgcac gccggtcggg ccccggatct
     5581 tcccggtgaa ctatgtctgt gaagggggcg actcgattgt cttccggacc agcccttcca
     5641 gcacgctcgg gacatttgcg tgggggactg aagtggcgtt ccaggtggac gagctggact
     5701 ggaccacgtc atccggctgg agcgtcgaag ccgtcggccg ccccgtcgtg gtgcacgacc
     5761 cggtcgagat cgccgaactg cgcgggcatg gtgagccgca cccgtgggcg ggtggcagcc
     5821 gtgagatgtt catccgcctc cggtggaagg agatcgccgg gcggtcggtc ggcaccgcgc
     5881 cgctgcccca ggagcctcgg gacctacggc ccgctgacga agaccactct gccctgtcgt
     5941 ggcacgatcg acgcaaggac catgaaggac catgagtgac accggaccgg gcaacggctg
     6001 gccaccgcgc aggctcgagg aactggcgcg tgccgagtgc gtgcggctgc tgggcagcaa
     6061 gtcggtggga cgcgtggcgt tcgttgccga aggctcgccg gtcgtgctgc cggtgaacta
     6121 cgtttccagc gacgggatgg tgtacttccg tacgtcgacg cacaacgtga tggcagccca
     6181 tctcctcgat gcggcagccg cattccaggt cgacgacgtc gacgagttca tggagtccgg
     6241 ctggagcgta ctcgttcagg gatgggctga attcctcgac ccggaggtgg agacggagta
     6301 cgggctcgaa cgcctcgcgc accggatcga accgtgggcc gcgggcgcca ggtacctcct
     6361 ggtccgcatc aagccagaac ggatctccgg acgccgtctc gagccacgct gagggcgggc
     6421 cttgcccgtc cggctcctcg gggcgcatca tcacggtgtt cggaggtcgg agggtgatcg
     6481 ctggtgatcg gggggtgatg gccgcacagg tccaggagtc gcggacgccc gcccggttcg
     6541 cgtacgtcgc gctcggagtc ggcgccgtgg tcgcggtcgt cctgggcgtg ttcgggaagg
     6601 cggcgcacga cgagggtcac gccgccgttc tcacgacggg gttcggcggg ctcgccacga
     6661 tgaaggtgta cctctcgacc gtcgtggcgg tcctggccct cgtccagatc gtcaccgccc
     6721 tctggctcta cggacggctc gggtcgcgga tccctcgaca tctcggcgtg gttcacaaga
     6781 cgtcgggcgc gctcgcggtg ctcctcagca tcccggtcgc gttcaactgc ctgtggttgc
     6841 tgggtttcga gtcctacaac ttccgtgtcg ccatgcactc gattgccggc tgcgtcttct
     6901 acggagcgtt cgttgccaag gtgatggggc tgcatgcgag gcgggcaccg gggtggctgg
     6961 tgactgtcgc agccggcctc gtgttcgccg ccatcgtcgt cgtggtgtgg accggggcgg
     7021 tctggtacgt cggcgagttc ggctggccga gcacgtccac cggagagtga gtcctgttgc
     7081 gccaggcctc agcgagtcat tcgtcgtcgt ggtcgcggtt ccaggtggcg cggccgaacc
     7141 ggcgcttgag tcgcgccatc atggaggtcc cgcgcatccg acggggatct gcacggtagg
     7201 tcggggcgct gtcgggtagc ggaagcgtcg cggcctcgct cgttgtcaga gcacagtgca
     7261 cggcgaggtt ggtgatgcgg tcgatctggt cgcggtcgac gaagcggtcg cctcccggcg
     7321 tctcgaccac gatgccgtcg aacagatcca ggtcggggat gtcgagaacc cgctcgaccg
     7381 tcccgatcac gtcgccgtcg ctggagagta cctgcgtgcc ccgctgcagc gcggtgtacg
     7441 cgatcggatg ctcttcggtc atggtgttgc aacctactcg tacgccgacg gcgcccaacg
     7501 acgccaccgg cgtacggaag actgcggctc agccacacgc ctgctgcgcc acggtgaacg
     7561 gcttgtcagg acagcgcctt gctcttgccc gactggaact cctcctcggt gatcacgccc
     7621 ttgtcgcgca ggtcggcaag tctcgcgatc tcgtccgctg tgccgccgcc gccaccaccg
     7681 ccggagccgg cgaccgagcg aatgtactcg cgggtggcgg cgtcctgctg ctgtgccgcc
     7741 tgcatggccc gtgcgctcat cttgtgaccg cgcgcgatga ggtacaccag gaccccgagg
     7801 tacggcagga tgatgatgaa gatgatccac agcgccttgc cccatccgga gaggtcatcg
     7861 ctgcggaaga tgtcaccgaa gacgacgatc agcagccaga tccagatgaa gaacagcgtc
     7921 agccagagaa tcgacaagaa gacgtcgccg tctccgaagt cgtctgccaa aaccatgagt
     7981 tcgaacctcc ggttcgtggt atcgggcacc gcgtcgccac cggcccgttg ccggacatcg
     8041 gcttcgcccg ctcactcgaa agggtgaccc gtgaactgac ggctcgcctc gcccggaaag
     8101 ggtgaaccga acctccgacg cgggacacta gggcgagagg aacctgcctg ccagcgtggg
     8161 tccgagccac ggccggcgtc cccacgcacg cagcgcgccc cgtcaggatg tagggccgct
     8221 gcgacgtccg tttgtagagg accagcaaca tggcggtggc gtcgccggag atgtggcagt
     8281 cgatccgcgg cgcgggcgtc gggtcgccag tcggcgtgcc gtcgacgaac gccagtacca
     8341 ccggcgcggg gagtccggcg aggcggagct cgaaggctgc tgtcagaccg cgggtgtgga
     8401 actcgtcgag ccagccgggc gcgatccagc acaccgcgtc gaggaccatc gccgcccgat
     8461 ccgagcgggt cggccacggg ggccgcggca tgcgcgatgt cgcgcccgtg aaccaacagc
     8521 tcgcccaaca agacccccag ggcaagggcc atcgcaatct ctccccgtcg acggcaactt
     8581 tcaccggagt accagcccac ccgactggtg gcaagcgccg acgggggtca aaccgactgg
     8641 cgtgctccgg caaggacgcg tccgacgttg gcgtcggaga agaagtcgac aggcgaca
//
LOCUS       DATBFZ010000213        10090 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_4666540, whole genome shotgun sequence.
ACCESSION   DATBFZ010000213 DATBFZ010000000
VERSION     DATBFZ010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 10090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10090
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4666540"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..991)
                     /gene="clpX"
                     /locus_tag="VH419_06415"
     CDS             complement(<1..991)
                     /gene="clpX"
                     /locus_tag="VH419_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014816688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEY7043285.1"
                     /translation="MARIGDGGDLLKCSFCGKSQKQVKKLIAGPGVYICDECIDLCNE
                     IIEEELSEGGELGLEELPKPREIYEFLNGYVIGQDAAKKSLAVAVYNHYKRVQAGVAS
                     AMASRGKDEAVELAKSNILMIGPTGCGKTYLAQTLARMLNVPFAIADATALTEAGYVG
                     EDVENILLKLIQAADYDVKKAETGIIYIDEVDKIARKSENPSITRDVSGEGVQQALLK
                     ILEGTTASVPPQGGRKHPHQEFIQIDTTNVLFIVAGAFAGLEKIIQDRVGKRGLGFGA
                     EIRSKAEIDTADFFADTMPEDLIKFGLIPEFIGRLPVVASVTNLDKASLVQILT"
     gene            complement(1105..1728)
                     /locus_tag="VH419_06420"
     CDS             complement(1105..1728)
                     /locus_tag="VH419_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEY7043286.1"
                     /translation="MTQPRAEYFIPQWEERTSYGVRRIDPFAKLFEDRIIFLGTPISD
                     DIANAVMAQLLCLQSMDPDRDISIYINSPGGSFTALTAIYDTLRYLKPDVQTVCLGQA
                     ASAAAVLLAAGTKGKRLALPNSRILIHQPYTEGTYGQASDIEIQANEILRMRTLLEEM
                     ISENTGKSTEEVSRDIERDKILTAEQAIEYGLIDQVLESQKATPAPA"
     gene            complement(1809..2420)
                     /locus_tag="VH419_06425"
     CDS             complement(1809..2420)
                     /locus_tag="VH419_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012919916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEY7043287.1"
                     /translation="MNGAGNGAGLDDRIYERLLRERIVFLGSEVKDENANAICAQMLL
                     LSAEDPKSDIFLYINSPGGSVDAGMAIYDTMQYVPNDVATFGMGLAASMGQFLLCAGA
                     KGKRHALPHARIMMHQPSGGMGGSASDIKIQAEQSLYIKKVLFDLISKHTGQPLEQVE
                     RDADRDRWFTADQAQEYGFIDHVVEQASDVTAGKNGRGKGGFE"
     gene            2590..3186
                     /locus_tag="VH419_06430"
     CDS             2590..3186
                     /locus_tag="VH419_06430"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEY7043288.1"
                     /translation="MPDVPDISGEPGLPRRPVGAEPTPEGLRIRPAARAVVMDTDSRV
                     LLVHFNFGPDDLPTGLWACPGGGIDPGESVEQGLVRELREEIGIDIGDPGPVVWWKEH
                     VFPMTRWDGQQDTYFWIRVPPFEPRPAFTEAELRAEKVDGMRWWTFAEILHAQRLYDE
                     DRVDDPAYTTFSPRRLGHLLEDLLREGHPPEPIVLPPL"
     gene            complement(3218..4099)
                     /locus_tag="VH419_06435"
     CDS             complement(3218..4099)
                     /locus_tag="VH419_06435"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY7043289.1"
                     /translation="MLREAEHTTGGEGASRLLTPVSPDDPEYALNRDAEEYDRLMEQA
                     AVVAPLSRRVLEHAGIEAGMTVLDVGCGVGDFSMLLGDLVGPTGQVVGVDLDANALAL
                     AADRAEAAGLTQLRFVHGDCREVELYQQFDAVAGRFVLMYQADPVDALRRVSRHVRAG
                     GIVAFHELDLDVVPPADPPLSLWHEVVDAIVAAFRASGAKARMARELHATFRAAGMAD
                     PVLNGEVIVAGGSTSPAYSWVANTLKSVWPLGLRTGVVREGQFDLETLADQLRAEVVS
                     GGGVVCSPLYVGASARV"
     gene            4293..4790
                     /locus_tag="VH419_06440"
     CDS             4293..4790
                     /locus_tag="VH419_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043290.1"
                     /translation="MSTTWLVAAGLIAALAAIVAVLALRKARRRRALARTAHELGLRF
                     SEGDPLGLSRDLGAEVSETIWGRFEGADVAIATASRAVHRSDSETWETGPIGVSLKIK
                     VVRGLEVAQQAAGPATQVAMTHDGRLAVKPRIPRQRSAPTVLDPDKLPDLLQATARAA
                     RSQYW"
     gene            complement(4801..5442)
                     /locus_tag="VH419_06445"
     CDS             complement(4801..5442)
                     /locus_tag="VH419_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020578166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY7043291.1"
                     /translation="MRVILAEDHALLRDGLTRLLEANGCTVIAAVDNAVSLDKALREL
                     ETDVAVVDVRLPPTFTDEGLRSAIAARTERPGLPVLVLSQYVEQLYARELLESGEGAV
                     GYLLKDRVSDVSSFVDAVRRVAGGGTVLDPEVVSTLLARRSGAPIERLTPREREVLGL
                     MAEGRSNAAIAARLFVTEKAVSKHTNNIFAKLDLGQDQDDHRRVLAVLAYLQS"
     gene            complement(5511..6836)
                     /locus_tag="VH419_06450"
     CDS             complement(5511..6836)
                     /locus_tag="VH419_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012922567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing protein"
                     /protein_id="HEY7043292.1"
                     /translation="MSSTTMDPPGQPQHKPQYAEEAIMSGSLQRERGRLGRLASTGLA
                     VAYMLLAIPALALFVIVSTAIPLVIITVGALMLLVFVPVVAALAQAHRHIASRVLLEP
                     VRPFYKPTKGLGVLGRLQRWAGDPARWRDYVWLFVSSTVGFTLGLIGTVLFLSIFWYL
                     IYPFLFAVTPEGVFEEDLGFMVIDTQQETFLMWIGALVFFLLWWICIKPLVRLQAVID
                     RALLSSRTQRLEQRVEHLSTSRAATVDQSAAEIRRIERDLHDGAQARLVSLGMSLGMA
                     DELLAHDPEAARKMLSEARLTTSAALGDLRSVVRGIHPPVLADRGLGGAVQALAMDMA
                     IPVTVTVMLAKRPPAPVESAAYFAVAESLANIGKHANASRAWISVEQRNSDLHIEVGD
                     DGRGGANPESGTGLRGVVRRLSAFDGTLALSSPDGGPTLVTVDIPRAFS"
     gene            7017..9209
                     /locus_tag="VH419_06455"
     CDS             7017..9209
                     /locus_tag="VH419_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020392295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEY7043293.1"
                     /translation="MTERKRPQLTLRAARWSAEHPWRALLVWVAFVAAAVAAGSAIAT
                     QETTDADYRLGQSGRADQIIADAGLADPMAENVLITAGHGTLASAEAEAAATDVRRGM
                     QPLDEVKDVGQPVWSKDHTALLVPITLVSDEDDADVSALQQVTASVQQQHTDLDIAQT
                     GDASLNNAINDRVSHDLSAAEGLSLPVTLVIMLLAFGALIAAGIPVLLAISGVLATIG
                     IYAPISYLVPSEPTVSSMILLIGMAVGVDYSLFYLKREREERERGRGTVEAVEIAAAT
                     SGHSIVVSGFAVIVSMAGLYVIGDVTFSSLATGAIIVVAVAVLGSITVLPALLAKLGR
                     WVDRPRVPLLWRVNRRIGRGGISRRLLAPVMRHPKAALLASSVVVVALAVPALGLRLG
                     EGNLQTLPQSIPEVQTAQQLEQRFPSEGSVLQVAVQSDGSSDDAVPALQKLAADAASR
                     GDVVPASSDPIRTSADGRTAVLSLSVPTADADQGTEALDLLRNDLAPNLLGPLHGSEW
                     AVGGDIAESADYTNQANDALPLVIGFVLLLTLLMMGYTFRSVPIALITTVLNLASVAA
                     AFGLLSLVFQHSWAEGLLGFESSGFVSDWIPMFLFVVLVGLSMDYHVFVLSRVREGVE
                     RGLPSKVAVEAGITETAGVVTSAAAVMVSVFAIFATMSLFELKEMGVGLAAAILIDAT
                     LIRVVMLPSILSLMGDNVWWPRKVSRPRPVPVVDEHVPELEGVGAPSR"
     gene            complement(9262..9927)
                     /locus_tag="VH419_06460"
     CDS             complement(9262..9927)
                     /locus_tag="VH419_06460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HEY7043294.1"
                     /translation="MEQVWYAGYGSNLAWARFRCYLQGGRPEGAARTLPGATDPADPL
                     DMQPITLDGSVYFAWHSPTWGGGIAFYDPDAGGTSLGRAYLVTAAQFVDVATQEMHRE
                     PTGDPLDLVALMDAGRLVLGPGRYETLHVVGEIGNQPVVTFTSSVHDEMPLNAPRAAY
                     LTTMARGLAETHGLDASAITSYLLGRPGCALGWTHDDLAALVSAALTAQGNSTATKGG
                     LPG"
BASE COUNT         1578 a         3415 c         3418 g         1679 t
ORIGIN      
        1 tggtgaggat ctgcaccagc gacgccttgt ccaggttcgt caccgacgcg accaccggca
       61 gacgcccgat gaactcgggg atcaggccga acttgatcag gtcctcaggc atggtgtcgg
      121 cgaagaagtc ggcggtgtcg atctccgcct tcgagcggat ctcggcgccg aagccgaggc
      181 cacgcttgcc gacacggtcc tggatgatct tctccaggcc cgcgaacgca ccggccacga
      241 tgaacagcac gttcgtggtg tcgatctgga tgaactcctg gtgcgggtgc ttgcggccac
      301 cctgcggggg caccgaggcg gtggtcccct ccaggatctt cagcagcgcc tgctgcacac
      361 cctcgccgga gacgtcccgg gtgatcgacg ggttctcgga cttccgggcg atcttgtcga
      421 cctcgtcgat gtagatgatc ccggtctcgg ccttcttcac gtcgtagtct gcggcctgga
      481 tcagcttgag caggatgttc tcgacgtcct caccgacgta gcccgcctcc gtgagcgccg
      541 tcgcgtcggc gatcgcgaac ggcacgttga gcatgcgcgc gagcgtttgg gcgaggtatg
      601 tcttcccgca cccggtcggg ccgatcatca agatgttgga cttggcgagc tcgaccgcct
      661 cgtccttgcc gcggctcgcc atcgccgagg ccaccccggc ctggacccgc ttgtagtggt
      721 tgtagacggc gaccgcgagg gacttcttcg cggcgtcctg gccgatgacg tacccgttga
      781 ggaactcgta gatctcgcga ggcttgggta gctcctcgag gccgagctca ccgccctcgg
      841 agagctcctc ctcgatgatc tcgttgcaca ggtcgatgca ctcgtcgcag atgtagacgc
      901 cggggccggc gatgagcttc ttgacctgct tctggctctt cccgcagaac gagcacttca
      961 gcaggtcgcc accgtcacca atgcgtgcca cgcgtgacgt ctccttcgct gtggtgccag
     1021 ggctcggtgc gcatcggaag cacccctggc ctgctgtcca tgttgaccgt acccgtcagg
     1081 accgtcaaca gccggtagga cggctcaggc aggtgccggt gtcgccttct gcgactccag
     1141 gacctggtcg atcaagccgt actcgatggc ctgctcggct gtgaggatct tgtcgcgttc
     1201 gatgtcgcgg ctgacctcct ccgtcgactt gccggtgttc tcagaaatca tctcctccag
     1261 cagcgtgcgc atcctgagaa tctcgttcgc ctggatctcg atgtcgctgg cctgcccgta
     1321 cgtgccctcg gtgtacggct ggtggatcag gatccggctg ttcggcagcg ccagccgctt
     1381 gcccttggtc ccggccgcga gcagcaccgc ggcggccgag gcggcctggc cgaggcagac
     1441 cgtctgcacg tcgggcttga ggtagcgcag cgtgtcgtag atcgcggtca gcgcggtgaa
     1501 cgacccgccc ggcgagttga tgtagatgct gatgtcgcgg tcggggtcca tcgactgcag
     1561 acagagcagc tgggccatga ccgcgttggc gatgtcgtcg ctgatcggcg tcccgaggaa
     1621 gatgatgcgg tcctcgaaca acttggcgaa cgggtcgatc cggcggacgc cgtacgacgt
     1681 ccgctcctcc cactgcggga tgaagtactc ggcgcggggc tgtgtcatct gggtcagctg
     1741 gtgcaggggc acctgctcag gactggtcat cgaacctcca gaggcggcaa ggaagagcgg
     1801 aacgccggtc actcgaaacc tcccttgcca cgaccgttct tgccggccgt gacgtccgag
     1861 gcctgctcga cgacgtggtc gatgaagccg tactcctggg cctggtcggc ggtgaaccag
     1921 cggtcgcggt cggcgtcgcg ctcgacctgc tccagcggct gcccggtgtg cttgctgatc
     1981 aggtcgaaca gcaccttctt gatgtagagc gactgctcgg cctggatctt gatgtcggac
     2041 gccgaaccgc ccatgccgcc ggagggctgg tgcatcatga tccgggcgtg gggcagcgcg
     2101 tgtcgcttgc ccttggcgcc cgcgcagagc aggaactggc ccatcgacgc ggccagcccc
     2161 atcccgaacg tggcgacgtc gttcgggacg tactgcatgg tgtcgtagat cgccatccct
     2221 gcgtccacgg agccgcccgg ggagttgatg tagaggaaga tgtccgattt ggggtcctcg
     2281 gccgacaaca gcagcatctg cgcgcagatc gcgttggcgt tctcgtcctt gacctccgaa
     2341 ccgaggaaga cgatccgctc acggagcagc cgctcgtaga tgcgatcgtc gaggccggcg
     2401 ccgttgcccg cgccgttcat ctggatggag tctgagggag tcacggtaga agacacgagt
     2461 cgacactagc ggtcggcgac gacgaagcca cgggagttcg ctccgtgttc gctgtcggcg
     2521 cattcgaccg gccaaacgct gccgaatgtg cggtccttgc tgccgagcct gcggcctaga
     2581 ctgcccagcg tgcccgacgt ccccgacatc tccggcgaac cgggtctgcc gcgccgtcct
     2641 gtcggtgccg aaccgacgcc ggaggggctg cgcatcaggc cggcggcccg ggccgtggtc
     2701 atggacaccg acagtcgcgt cctgctggtg cacttcaact tcggccctga cgacctgccg
     2761 accggtctgt gggcgtgtcc gggcggcggc atcgatcccg gtgagagcgt ggagcagggc
     2821 ctggtccgcg agctccggga ggagatcggg atcgacatcg gcgaccctgg ccccgtcgtg
     2881 tggtggaaag agcacgtctt cccgatgacc cgttgggatg gtcaacaaga cacctacttc
     2941 tggatcaggg tcccgccgtt cgagccgcga ccggcgttca ccgaggcgga gctacgagcc
     3001 gagaaggtcg acgggatgcg ttggtggacg ttcgccgaga ttctccacgc gcaacggctg
     3061 tacgacgaag accgcgtcga cgacccggcg tacacgacgt tctcgccgcg gcgtctcggc
     3121 cacctgctcg aagacctgct ccgggagggg catccgccgg agccgatcgt gctgccaccg
     3181 ctctgacgac gaccggctgg cggccggagc cgccggttca gacgcgggcc gaggcgccga
     3241 cgtagagcgg cgagcacacc acaccaccgc ccgacacgac ctccgcgcgc agttggtcgg
     3301 cgagtgtctc gaggtcgaac tgaccctctc ggacgacgcc ggtccgcaac ccgagcggcc
     3361 agacgctctt gagcgtgttc gccacccagc tgtacgcggg actcgtgctg ccaccggcca
     3421 cgatcacctc cccgttgagg acgggatctg ccatccccgc cgcacggaac gtggcgtgca
     3481 gctcgcgtgc catccgcgcc ttcgcgccgg atgccctgaa cgccgcgacg atggcgtcca
     3541 ccacctcgtg ccagagcgac agcggcgggt ccgccggcgg tacgacgtcg aggtccagct
     3601 cgtgaaacgc cacgatcccc ccggcgcgca cgtgccggct cactcggcgt agcgcgtcga
     3661 caggatcggc ctggtacatg agcacgaacc gtcccgccac cgcgtcgaac tgctggtaga
     3721 gctcgacctc tcggcagtcg ccgtgcacga accgcagctg cgtcaagccg gccgcctcgg
     3781 cgcgatcggc cgcgagggcc aacgcgttcg cgtccagatc gacgccgacc acctgccccg
     3841 tcggcccgac aaggtccccg agcaacatgc tgaagtcgcc cacgccgcag ccgacgtcga
     3901 gcacggtcat cccggcctcg atgcctgcgt gctcgagcac ccggcgtgac agcggtgcga
     3961 ccacggccgc ctgctccatc agcctgtcgt actcctcggc gtcccggttc agcgcgtact
     4021 ccggatcgtc tggcgatacg ggtgtcaaca gcctcgacgc gccctctccc cccgttgtgt
     4081 gttctgcctc gcgcagcatg gtgattcccc tcctcgatgt cgaccacccg ccgtcaaatg
     4141 tgtcgacgtt ggtgcgggct gccatgcggt caacaacgag catccgggac ggcagacggc
     4201 tcggaatcag ggataacccg cacattgcca cgggagcccc ccggcggcgt ggtgttgtct
     4261 ttgtgcgtcg gccgggccac catgcaagaa ccatgtcgac cacctggctc gtcgcagcag
     4321 ggctgatcgc cgcgctggcg gcgatcgtcg ccgtgctcgc gctgcgcaag gcacggcgcc
     4381 gccgtgcgtt ggcgcgtacg gcgcacgagc tcggtctgcg cttcagcgag ggcgacccgc
     4441 tcgggctcag ccgcgacctg ggagccgagg tgtcggagac gatctggggc cgcttcgagg
     4501 gggccgacgt cgcgatcgca accgcgtccc gcgccgtcca tcgctcggac agtgagacgt
     4561 gggagacagg acccatcggc gtcagcctga agatcaaggt cgtccgggga ctcgaggtgg
     4621 cgcaacaggc cgccggcccg gcgacccagg tcgcgatgac gcacgacggc agactggccg
     4681 tcaaaccccg gatcccacgc cagcggtcgg cgccgaccgt gctcgacccc gacaagctgc
     4741 ccgacctgct ccaggcgacc gcacgagccg cccgctccca gtactggtga tcacggacgg
     4801 tcaggactgg agataggcga gcacggcgag cacccggcgg tggtcgtcct ggtcctgacc
     4861 gaggtcgagc ttggcgaaga tgttgttggt gtgcttgctg accgccttct cggtcacgaa
     4921 caggcgggcg gcgatcgcgg cgttcgaccg accttcggcc atcaggccca aaacttcgcg
     4981 ctcgcgcggc gtcagccgtt cgatcggcgc acctgaccgc cgcgccagca gagtcgagac
     5041 aacctccgga tcgagcacgg tgccgccgcc ggcgacccgg cgtacggcgt ccacgaagga
     5101 cgacacgtcg gacacccggt ccttcagcag gtacccgacg gctccctcac cgctctcgag
     5161 gagctcgcgc gcgtagagct gctcgacgta ttgcgacagc accagcactg gtagtcctgg
     5221 gcgttcggtg cgagccgcga tcgccgatcg aagcccctcg tcggtgaacg tcggcgggag
     5281 tcgtacgtcg accacggcaa cgtcggtctc gagctctcgc aaggccttgt cgagcgacac
     5341 ggcgttgtcc acggctgcaa tcacggtgca gccgttggcc tcgaggaggc gagtcagacc
     5401 gtcccgcagc agcgcgtggt cttcggcgag gatcacccgc atacggggac cctacccacc
     5461 gcccttcgag ccgctggcgg gcggcgcaat ggacgtgccg tcgaggcggc tcagctgaat
     5521 gcgcggggga tgtcgacggt gaccagggtg gggcccccgt ccgggctgga cagcgccagc
     5581 gtcccgtcga aggcggacag ccgtcgtacg acgcctcgca ggcctgttcc cgactccggg
     5641 ttcgcaccgc cgcgcccgtc gtcgcccacc tcgatgtgca ggtccgagtt gcgttgctcg
     5701 acactgatcc acgcgcggga cgcattcgca tgcttgccga tgttggccag ggactccgcg
     5761 actgcgaagt acgcagcgga ctcgaccggc gccggcggcc gtttcgccag catcaccgtc
     5821 accgtgacgg ggatcgccat gtccatcgcg agcgcctgca ctgcgccacc gagcccacga
     5881 tctgccagta cgggtgggtg gatgccgcgg acgacgctgc gcaggtcgcc gagggcggcc
     5941 gaggtcgtga ggcgcgcctc gctgagcatc ttgcgagcgg cctccgggtc gtgtgccaag
     6001 agctcgtcgg ccatgccgag gctcatgccg agcgagacaa gccgagcctg cgcgccgtcg
     6061 tgcaggtcgc gttcgatgcg gcggatctca gcagcggact ggtcgactgt cgcggccctc
     6121 gacgtcgaca gatgctcgac acgttgctcc aggcgctgcg ttcggctcga caacaacgct
     6181 cggtcgatca ccgcctgaag gcggaccagc ggtttgatgc agatccacca cagcaggaag
     6241 aacaccaacg ccccgatcca catcaggaac gtctcctgct gggtgtcgat caccatgaaa
     6301 ccgaggtcct cctcgaacac tccctccggg gtgaccgcga agaggaacgg gtagatcagg
     6361 taccagaaga tcgacaggaa gagcaccgtc ccgatcagac cgagtgtgaa cccgaccgtg
     6421 gacgacacga acagccagac atagtcgcgc cagcgcgccg ggtccccggc ccaccgttgc
     6481 agccgcccga gcaccccgag gcccttcgtc ggcttgtaga acggccggac cggctcgagc
     6541 aggacccggc tcgcgatgtg gcggtgcgcc tgcgcgaggg ctgcgacgac cggcacgaag
     6601 acgagcagca tgagcgcacc gacggtgatg atcacgaggg gtatggctgt cgagacgatg
     6661 acgaacagtg ccagcgccgg gatcgcgagc agcatgtagg cgacggccag accggtcgag
     6721 gcaagccttc ccagccggcc gcgctcgcgc tgcaggctgc cgctcatgat cgcctcctcg
     6781 gcgtactgcg gtttgtgctg aggctgaccc ggcgggtcca tcgtcgtgct cgacatgagg
     6841 tgcttccttc actcgttcgg tgcacccatc gtggtcgccg aggacagtcc acacagtagg
     6901 tctagcacca ctgttgttgt ggtcctgaca cccctgattc ccgccccgcg cgaaaccagg
     6961 ctggttgatg tcggcgagac gaccggcacg tcaacgagac cgaggggaac atcgagatga
     7021 ctgagaggaa acggccgcag ctgaccttgc gggcagcgcg atggagtgcc gagcacccct
     7081 ggcgagcact gctcgtctgg gtggcgttcg tcgcagccgc tgtcgcggca ggcagtgcga
     7141 tcgccaccca ggagaccact gacgccgact accgccttgg tcagtcgggt cgcgcggacc
     7201 agatcatcgc cgatgcgggt ctggccgatc cgatggcgga gaacgtgctc atcacggccg
     7261 gccacggcac gctcgccagt gctgaggccg aagccgcggc caccgacgta cgccgaggaa
     7321 tgcaaccact ggacgaggtg aaggacgtcg gtcagccggt ctggtcgaag gaccatacgg
     7381 cgctgctcgt cccaatcacg ctggtgagcg acgaggacga tgccgacgtc agcgcgttgc
     7441 agcaggtgac cgcatccgtc cagcagcagc acaccgacct ggacatcgca cagaccggcg
     7501 atgcatcact caacaacgcc atcaacgatc gggtgagcca cgacctctcg gcggcggaag
     7561 gcctcagcct gccggtcacg ctcgtcatca tgctgctggc gttcggcgcg ctgatcgccg
     7621 ccggcatccc ggtgctgctc gcaatctcgg gtgtgctcgc gacgatcggg atctacgcgc
     7681 cgatctcgta cctggtcccc agcgagccga ccgtgtcgag catgatcctg ctgatcggga
     7741 tggcggtggg cgtcgactac tcgctgttct atctcaagcg agagcgtgag gaacgtgaac
     7801 gcggccgcgg aaccgtcgag gccgtcgaga tcgccgctgc cacctctgga cactcgatcg
     7861 tcgtctcggg gttcgcggtc atcgtgtcga tggccggcct ctacgtcatc ggcgacgtga
     7921 ccttttcgtc gctggcgacc ggcgcgatca tcgtcgtcgc cgtcgccgtc ctcggctcga
     7981 tcaccgtgct gcccgccctg ctcgccaagc tcggccggtg ggtcgaccgg cctcgcgtgc
     8041 cgttgctctg gagggtgaac cgacggatcg gtcggggcgg tatcagccgt cggctgctgg
     8101 caccggtgat gcgccacccg aaggcggcct tgctcgcgtc gagcgtcgtc gtcgtggcgt
     8161 tggccgtgcc cgcgttgggc cttcgcctgg gcgagggcaa cctgcagacg cttccacagt
     8221 cgatccccga agtgcagacc gctcagcagc tcgagcagag gttcccgagc gagggatcgg
     8281 tccttcaggt ggccgtgcag tccgacggct cgagcgacga cgccgtaccg gcgttgcaga
     8341 agctggcggc cgacgcggct tcgcgcggtg atgtcgtgcc tgcgtcctcg gatccgattc
     8401 gcacctcggc ggacggtcgg acggccgtgc tctcgctgtc ggtgccgacc gccgacgctg
     8461 accagggcac cgaagcgctc gacctcctcc gcaacgacct ggctccgaac ctgctcggcc
     8521 cgttgcacgg ctcggagtgg gcggtcggcg gagacatcgc cgagtcagcg gactacacca
     8581 accaggcgaa cgacgcactg ccgctggtga tcgggttcgt cctgctgctc acgttgctga
     8641 tgatgggcta cacgttccga agcgtgccga tcgcactgat cacgacggtg ctgaacctgg
     8701 catcggtggc ggcggcgttc ggactgctga gcctggtgtt ccagcacagc tgggcggagg
     8761 ggctgctggg cttcgagtcg agcggtttcg tctccgactg gatcccgatg ttcctgttcg
     8821 tcgttctggt cggcctgtcg atggactacc acgtgttcgt cctgagccgg gtgcgcgagg
     8881 gggtcgagcg cggactcccg tccaaggtcg ccgtcgaggc ggggatcacc gagaccgcgg
     8941 gcgtcgtgac gtcagctgcc gccgtgatgg tgtcggtgtt cgcgatcttc gcgaccatga
     9001 gcctgttcga gctgaaggag atgggagtcg gcctcgcggc ggcgattctg atcgacgcca
     9061 cgctgatccg ggtcgtgatg ctgccgtcga tcctgtcgct catgggtgac aatgtctggt
     9121 ggccacggaa ggtgtctcgc ccccgaccgg tgccggttgt cgacgagcac gtccccgagc
     9181 tcgagggtgt tggtgctccg tcccgttgac agttcccttt ccaaggtggg ggtttgcctg
     9241 gtcagccatc cgaacgcacg gctaaccagg caaacctccc ttggtcgcgg tggagttccc
     9301 ttgggcagtg agcgcggcgg aaaccagggc ggccaggtcg tcatgagtcc agcccaacgc
     9361 gcagccggga cggccgagca ggtacgacgt gatcgcgctc gcgtccagcc cgtgcgtctc
     9421 ggccagaccg cgggccatgg tcgtgaggta tgccgctcgc ggagcgttca acggcatctc
     9481 gtcatggacc gacgacgtga acgtcacgac gggctgattg ccgatctcgc cgacgacgtg
     9541 cagcgtctcg taccttcccg gaccgagcac gagccggccg gcatccatca gcgccacgag
     9601 gtcgagtggg tcgcccgtcg gctcccggtg catctcctgg gtggcgacgt cgacgaactg
     9661 cgcagcggtg acgaggtacg cgcgaccgag cgaggtgcca ccggcgtcag ggtcgtagaa
     9721 cgcgatccca ccaccccacg tcggcgagtg ccacgcgaag tacaccgagc cgtcgagagt
     9781 gatcggctgc atgtccagcg gatcggcggg gtcggtggcg ccagggaggg tgcgcgcggc
     9841 accctcgggc cgcccgcctt gcaggtagca gcggaacctg gcccaagcaa ggttggagcc
     9901 gtagccggcg taccagacct gctccatccc ggcagtgcct acgtgtcgtc gcgctccggc
     9961 tctgcctcga ccaggtagtc ggtcgaggtc acgacggccg cggcgtccgt ggacggctcg
    10021 tcggcgacca ccgactccgc gccacctgcc tcggcgccgt ccatggaggg atcggcggac
    10081 gtcccgtcag
//
LOCUS       DATBFZ010000214        10376 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_139243, whole genome shotgun sequence.
ACCESSION   DATBFZ010000214 DATBFZ010000000
VERSION     DATBFZ010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 10376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10376
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139243"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..937
                     /locus_tag="VH419_06465"
     CDS             <1..937
                     /locus_tag="VH419_06465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043295.1"
                     /translation="WIKTKNKAGKEVVVAGGEDGWVSIRNSEGAEVVSIGGAAGTGDL
                     YLRNSSGQFTIALRAVEGHNAGAWIGGTGQTGWVTVRNKAGKDVAVVGGEDGWVSVRN
                     AEGSETVALGGDSGTGDVYLKNASNKFTIALRAVEDKRAGIWVGGNGQDGIIVLRDAE
                     GEDQVWLDGKAGDIVLVNADCAEEFDVQQPAEPGTVLVLGDTPGKLSASSSPYDTRVA
                     GVVASAGSQRPGILLGRREGPGRAAVALTGRTYCRVEADTAAIGAGTLLTTSSVPGHA
                     MAATDRDRAFGAVLGKALTGLGRGRGLLPVLVALQ"
     gene            complement(983..1921)
                     /locus_tag="VH419_06470"
     CDS             complement(983..1921)
                     /locus_tag="VH419_06470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043296.1"
                     /translation="MTKRSLRTSLGAIKAPAGVRVPSGFSGPRPASLRELIRACKRPR
                     MSEWGNPERAFAENKDWTEELNGLVTDGHAWYASCNAEDDREGLYKLTMGFGFVRKLE
                     HGFDVDERHIGALAVRNGKLFVPVQTDTRGVWIVDTDLTAGQYFPADKLEDDDLFAWC
                     DVHPRNGLIYTCNFSHPKRLFAYDISGSKLVRVKKADIPLRQPKDGKPTTEVQGGCFT
                     PNFKWLAVCDVDEAERIHCHSTLTGALLDRRTLLAEADESSVGLRNELEGIWYHPMHT
                     GAGSHVHVHVLELNNEHHTADDMYLWQFSLPASDVL"
     gene            2281..2934
                     /locus_tag="VH419_06475"
     CDS             2281..2934
                     /locus_tag="VH419_06475"
                     /inference="COORDINATES: protein motif:HMM:NF023144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HEY7043297.1"
                     /translation="MALSFSGVLDRAAVVRASDQLVDLVAADATAAAWERESALPGMT
                     VGGLARHLVSQPECAAEFLAIELPAQTETVPLVEYYERVDWLDAGIEEEANTSIRDGF
                     NALGAQGLDASLAVLDRSRTDLVAAIIAAGPATYVPWQGCALDTDDFLVCRLMEIVVH
                     ADDLATSVGLPTLPFDDDVYGPVLGLLAALSARRHGAQAVLRSLARTERAGGSISAF"
     gene            complement(2961..3033)
                     /locus_tag="VH419_06480"
     tRNA            complement(2961..3033)
                     /locus_tag="VH419_06480"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2998..3000),aa:Ala,seq:tgc)
     gene            complement(3090..3218)
                     /locus_tag="VH419_06485"
     CDS             complement(3090..3218)
                     /locus_tag="VH419_06485"
                     /inference="COORDINATES: protein motif:HMM:NF038356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DLW-39 family protein"
                     /protein_id="HEY7043298.1"
                     /translation="MLKKIIVIAVVAAIAKIVLNKLTARDKDAELWAEATDDVEAS"
     gene            complement(3306..3379)
                     /locus_tag="VH419_06490"
     tRNA            complement(3306..3379)
                     /locus_tag="VH419_06490"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3343..3345),aa:Ile,seq:gat)
     gene            complement(3469..4164)
                     /locus_tag="VH419_06495"
     CDS             complement(3469..4164)
                     /locus_tag="VH419_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017933483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3566 domain-containing protein"
                     /protein_id="HEY7043299.1"
                     /translation="MSNPGDTGPLAPRRPLSKSGASTSPTPGRVPAHNAASRTVSDAP
                     TQPVLTPPYGGNPQPYAAVEVEPGRAFPVPPAPDQEGDHRRPAPQPDKKKRRAERRAA
                     SRRPRTVRRAKLKLIRVDPWSVTKAALLLSIAFGIMCVVAVFLIFSIMNAAGLWDHVN
                     ETIQGVLNQDPQDAFDVKDYVGMNRVMGITMLISAIDVVLITALATLGAFIYNMAASL
                     LGGIEVTLAEDVK"
     gene            complement(4161..7514)
                     /gene="gyrA"
                     /locus_tag="VH419_06500"
     CDS             complement(4161..7514)
                     /gene="gyrA"
                     /locus_tag="VH419_06500"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002524570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEY7043300.1"
                     /translation="MSDDGTTTPEAPPGGRIEPVELQTEMQRSYIDYAMAVIVGRALP
                     DVRDGLKPVHRRVLYAMYDGGYRPDRGFSKCSRVVGDVMGQYHPHGDTAIYDTLVRLS
                     QPWVMRNPLVSGQGNFGSAGNDRPAAMRYCVTGETRVRTAEHGTMRIRDIVPDAAPGS
                     ETEIDLKVIGKDGDPVRATKFFHSGAQRTLRLRTTSGHQLTGSTNHPVLTLNSLAGVP
                     TLLWKLLEEVEAGDRVVMLREAMADSQQPASSASADAVPFVAEFLRRHAARDRQSQYW
                     LQQHNIDRLERWERDRVEILDRITAEGAEVVWPLVDGRFLYAEVAGVTDAGIQDVFSL
                     RIDTDDHAFITNGFVSHNTECKMASLAMEMVRDIDEDTVDFRPNYDGRSQEPVLLPAR
                     FPNLLVNGSAGIAVGMATNIPPHNLREVNDGVQWALANPTATRQELQDALIERVKGPD
                     FPLGALIVGTQGIEEAYRTGRGSVTMRAIVDIEEDSKNRTCLVITQLPYMVNPDNLAL
                     KIAELADSGKVQGIADVRDDSSSRTGQRLVIVLKRDAVARVVLNNLYKHTQLQDNFSC
                     NMLALVDDVPRTLSIDQFISNWVAHQIDVIQRRTRYRLSEAEKQAHVYRGLVKALDAL
                     DGVIALIRRSQTTDEARDELMTFLDIDEVQARAILDMQLRRLAAMERQRIVDALAELE
                     ETIADLEAILASEERQRRIVSEELQSIVDKFGDERRTQIIPADGDLSMEDLVPDEDIV
                     VTITRGGYAKRTKADLYRLQRRGGKGVRGAALKGDDLVDHFFASTNHHWLLFFTTAGR
                     VYRTKAYHLPEAARDAKGSHVAGLLSFQPDEQIAQVLAIRDYEQAPYLVLATRKGYAK
                     KTRLSEYNSPRQAGVIAINFRDDEDELIGAELVTADDDLLLVSRKGQSVRFRADDTQL
                     RPMGRATSGVTGMKFREGDELLSMSVVPHAPDADAPTRYVFTVTDGGWAKRTVVEEYR
                     VQGRGGLGIKAMKLADSRGSLVGAMVVTDTDEVMAIKASGQVTRSPAADVPAKGRDTM
                     GVRFVTVADSDTVVGIARNTEREVDEELFDDAVDAGSVAVEDADPTVSDRDGTPAEAT
                     TPPAGDTGDDVPDSSTPQQDTAPQEDGSE"
     gene            complement(7636..9696)
                     /gene="gyrB"
                     /locus_tag="VH419_06505"
     CDS             complement(7636..9696)
                     /gene="gyrB"
                     /locus_tag="VH419_06505"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020386712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEY7043301.1"
                     /translation="MPPADPELDGDNPAYDASQITLLEGLEAVRKRPGMYIGSTGERG
                     LHHLVTEIVDNAVDEALAGYCDLIEVTLLAGGGVRVVDNGRGIPTGIHPTEGVPAVTL
                     VLTELHAGGKFGGGGYKVSGGLHGVGSSVVNALSERLFVEVKNDGYRWTQSFSYGVPD
                     GDLQRHEATDETGTTVTWWPSRTIFETTDHSFETLASRFREMAFLNKGLEIVVRDERP
                     EHASGIAEAVADPTVDSSIDGGADRIRTDVTGSGGIEQRFRYQDGLIDYVGHLNKTKD
                     PAHRSIIAFEAANDEAGMSLEVAMQWNTGFTESVHTFANTINTHEGGTHEEGFRSALT
                     STVNNWAESWGLIKKKEDRLSGDDVREGLTAIVSVKLAEPQFEGQTKTKLGNTEAKSF
                     TQRVVNDRLGEWFEKNPTEGRDIIRKGIAAATARVAARKARDLARNRKGLLGGGGLPG
                     KLSDCQSTDPEECELFIVEGDSAGGSARGGRDPRIQAILPIRGKILNVEKARIDRVLA
                     NTEVQAIISALGTGIQEDFDVDKLRYHKIVMMADADVDGHHIQTLLLTLLFRFMKPLI
                     ELGHVYLARPPLYRLRWNNPHEHEFVYSDRERDALIEAGRAEGKKLPKDNAIQRYKGL
                     GEMNAEELWETTMDPDNRVLSQVTLDDAAHADEIFSILMGEDVEQRRSFIQRNAKDVR
                     FLDI"
     gene            complement(10203..>10376)
                     /locus_tag="VH419_06510"
     CDS             complement(10203..>10376)
                     /locus_tag="VH419_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011753506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HEY7043302.1"
                     /translation="AWATQLKLLAPTVVRRLNEELGDGTVVRIEVVGPASPSWRRGPR
                     TVRGGRGPRDTYG"
BASE COUNT         1612 a         3434 c         3445 g         1885 t
ORIGIN      
        1 ttggatcaag accaagaaca aagccgggaa ggaggtggtc gtcgccggtg gagaagacgg
       61 ttgggtgtcg atccgcaact ccgaaggtgc ggaggtcgtc tcgatcggcg gcgcggcggg
      121 caccggcgac ctatacctgc gcaacagctc cggccagttc accatcgcgc tgcgagcggt
      181 ggaagggcac aacgcagggg cctggatcgg tgggaccggt cagacgggct gggtcaccgt
      241 ccggaacaaa gccggcaagg acgttgccgt cgtcggggga gaggatggct gggtctccgt
      301 tcgcaacgcg gaaggttccg agaccgttgc gctcggtggc gactcgggca ccggcgacgt
      361 gtatctgaag aacgcgtcga acaagttcac gatcgcgctg cgcgctgtcg aggacaaacg
      421 agccgggatc tgggtcggcg gcaacggcca ggacggcatc atcgtgctca gagacgccga
      481 gggcgaggac caggtgtggt tggacggcaa agccggtgac atcgtcttgg tgaatgccga
      541 ctgtgcggag gagttcgacg tacagcagcc cgccgaaccc ggcacggtcc tggttctcgg
      601 cgacacacca gggaagctgt ctgcttcgtc cagcccgtac gacacccgag tcgccggcgt
      661 ggtcgcctct gccggctccc aacgccccgg gatcctgctc ggtcgccgcg aaggtccagg
      721 acgtgccgcg gtggcgttga cgggaaggac gtactgccgc gtcgaggcgg acaccgccgc
      781 gatcggcgcc gggacgttgc tgacgacgtc gtcggtcccc ggccatgcga tggcggcgac
      841 cgaccgggac cgagcgttcg gagcggtcct gggcaaagca ctgacgggcc tcggtcgcgg
      901 ccgcggcttg ctgcccgtgc tggtcgcgct gcagtgagag cagtcgggat acggacgctt
      961 ggcgagatcc tgggcagtcc tgctagagca cgtctgatgc cggcagcgag aactgccaca
     1021 ggtacatgtc gtctgccgtg tggtgctcgt tgttgagctc aagcacgtgg acgtgcacat
     1081 ggctgccagc gcccgtgtgc atcgggtggt accagatccc ttcgagctcg ttgcgaagtc
     1141 caaccgaact ctcatcggct tcggccagca aggttcgccg atcgagcaag gcgcccgtca
     1201 acgtgctgtg acagtggatg cgttcggctt cgtcgacatc gcacaccgcc agccacttga
     1261 agttcggcgt gaaacagcct ccctgcacct cggtcgtggg cttgccgtcc ttcggttgac
     1321 gcagcgggat gtcggccttc ttgacccgga cgagcttgct gccggagatg tcgtaggcaa
     1381 agagccgttt cgggtgcgag aagttgcagg tgtagatcaa cccgttgcgc ggatggacgt
     1441 cacaccaggc gaacaggtcg tcgtcctcca gcttgtcggc cgggaagtac tgccccgctg
     1501 tcaggtcggt gtcgacgatc cagacccctc gcgtgtcggt ctgcaccggg acgaacagct
     1561 tcccgttgcg gacggccagc gcaccgatgt gccgctcgtc cacgtcgaac ccgtgctcga
     1621 gcttgcggac gaacccgaac cccatcgtca gcttgtagag cccctcacgg tcgtcctcgg
     1681 cgttgcacga cgcataccag gcgtggccgt cggtcacgag cccgttgagc tcctcggtcc
     1741 agtccttgtt ctcggcaaac gcgcgttcgg ggttccccca ttcgctcatg cgcgggcgct
     1801 tgcaggcgcg gatgagctcg cgcaggcttg ccggccgcgg gccgctgaag cccgacggca
     1861 cacgtacgcc ggcgggcgcc ttgatggcgc cgagagatgt gcgcaggctc cgtttggtca
     1921 tgatgtgccc ccgatactcg gacgatacgc gcgcagagca agcggctcgt cgcacccaca
     1981 cctacctccg aagctgacga ctgttcactg acagcctgct ggcgcggttt gcgatgttta
     2041 cagcccaaaa ggcgtgtaga catcgcaaac ctccgatggg gacagcgggt ctcggatgtg
     2101 cacatcgcac atcacaacgc ccgattcgtg cagcagcacc gcaccttcgc cgccgccggc
     2161 gtaggccgcc catgacgcca ccagtgtctg agctccacgc cgatagagct cgtcgtcggc
     2221 aaacgtggcc acgaggcatc gttgcaggat cctgcacggg cagaccgcta aggtcacgcc
     2281 atggccctct ccttctccgg cgtgctggac cgcgctgccg tcgtccgcgc ctcagaccag
     2341 ctcgtcgacc ttgtcgccgc cgacgcgacc gctgcggcgt gggagcgcga atcggcactc
     2401 ccaggcatga cggtcggcgg gttggcgagg catctcgtca gccagccgga gtgcgcagcc
     2461 gagttcctcg ccatcgagct gccggcgcag accgagacag tgccgctggt cgagtactac
     2521 gagcgcgtgg actggctcga tgccggcatc gaggaggaag cgaacacctc catccgcgac
     2581 gggttcaacg cgctcggtgc ccaaggactg gacgcgtccc tcgcggttct tgaccggtcg
     2641 cggaccgatc tcgtcgccgc gatcatcgcc gcaggtcctg cgacgtacgt cccctggcag
     2701 ggctgtgcgc tcgacaccga cgacttcctc gtctgccggc tgatggagat cgtcgtccac
     2761 gccgacgact tggcgacgag cgtcggactg ccgacgttgc ccttcgatga cgacgtctac
     2821 ggcccggtgc tcggcctgct cgcagccttg tcggcccgcc ggcacggagc acaggccgtc
     2881 ctgcgatcac tcgctcgcac cgaacgggct ggcggctcga tctcagcatt ctgaaccggc
     2941 gatggagtct ctgaccgaag tggagacgaa gggactcgaa ccctcaaccc cctgcttgca
     3001 aagcaggtgc gctaccagtt gcgccacgtc cccgaggacc ggcccgttgg ccggcacgcg
     3061 cgccgtcgta cgacgacgca cacggtgggt cagctggcct cgacgtcgtc ggtggcctcg
     3121 gcccacagct cggcgtcctt gtcacgggcg gtgagcttgt tcagcacgat cttggcgatc
     3181 gcggccacca cggcgatgac gatgatcttc ttgagcatgg gtgctccctt gcacgatggt
     3241 cggtctggat tgcgtcggaa cccgcagcca gacccccgtg gagcctgcgg ccagaatcga
     3301 cacagtgggc ctaagaggac ttgaacctct gacctcttcc ttatcaggga agcgctctaa
     3361 ccgtctgagc tataggcccg gggcttcgat ctccacgcag gagggtcgcc gacgaaggtt
     3421 accccatcca gcaggtcgct cccaaaccag atcctggccg caggggcgct acttcacatc
     3481 ctccgcgagg gtgacctcga tgccaccgag cagggacgcg gccatgttgt agatgaaggc
     3541 accgagcgtg gccagtgccg tgatgaggac gacgtcgatg gcgctgatca gcatggtgat
     3601 gcccatcacg cggttcatcc cgacgtagtc cttgacgtcg aacgcatcct gcggatcctg
     3661 gttcagcaca ccctggatcg tctcgttgac gtggtcccag agccctgctg cgttcatgat
     3721 cgagaagatc aggaacaccg cgacgacgca catgatgccg aacgcgatcg acagcagcag
     3781 cgcggccttc gtcaccgacc acgggtcgac ccggatcagc ttgagcttgg cacggcgcac
     3841 cgtccgcggc cgccgcgagg cagcccgtcg ctcggcacgc cgcttcttct tatcgggctg
     3901 cggtgcgggc ctgcggtggt cgccctcctg gtctggcgcg ggcggtacgg ggaaggcgcg
     3961 tccgggctcc acctcgaccg cggcatacgg ctgcgggttg ccgccgtacg gcggggtgag
     4021 gaccggctgg gtcggcgcgt cggagacggt cctgctcgcc gcgttgtgcg cgggaacgcg
     4081 acctggcgtc gggctggtgg acgccccgga cttgctcagc ggccgccgag gtgccagcgg
     4141 cccggtgtca ccggggttgc tcattcggac ccatcctcct gtggtgccgt gtcctgctgt
     4201 ggtgtcgacg agtcgggtac gtcgtcaccc gtgtcacctg ccggaggcgt cgttgcctca
     4261 gccggcgtac cgtccctgtc cgagacggtc gggtcggcgt cctccacggc cacgcttccg
     4321 gcgtcgaccg cgtcgtcgaa gagctcctcg tcgacctctc gttcggtgtt gcgcgcgatc
     4381 cccaccaccg tgtcggagtc agcgacggtg acgaacctga ctcccatggt gtcacgtccc
     4441 ttggccggaa cgtcggcagc gggggagcgg gtcacctgcc cgcttgcctt gatcgccatg
     4501 acctcatcgg tgtcggtgac caccatcgca cctacgagcg atccacgcga atccgcgagc
     4561 ttcatcgcct tgatccccaa gccgccgcgg ccttgtacgc ggtactcctc cacgacagtt
     4621 cgcttcgccc agccgccgtc tgtgacggtg aacacatagc gggtcggggc atctgcgtcg
     4681 ggtgcatgcg gaacgaccga catcgacagc agttcgtcgc cttcgcggaa cttcatcccg
     4741 gtcacgcctg acgtggcccg gcccatcggc cgaagctgtg tgtcgtcggc acggaaccgc
     4801 accgactgac ccttgcgaga caccagcagc aggtcgtcgt cggcagtcac cagctcggcg
     4861 ccgatcagct cgtcctcgtc gtcgcggaag ttgatcgcga tgacgccggc ctggcgtggc
     4921 gagttgtact cactcagtcg ggtcttcttg gcatagccct tgcgcgtggc cagcaccagg
     4981 tacggcgcct gttcgtagtc acggatcgcc agcacctggg cgatctgctc gtccggctgg
     5041 aaggacagca gcccagccac gtgactgccc ttcgcatcgc gcgccgcttc cgggaggtgg
     5101 taggccttgg tgcggtagac acgcccggcc gtggtgaaga acaacagcca gtggtggttg
     5161 gtcgacgcga agaagtggtc gacgaggtcg tcacccttga gcgcggcgcc gcgaacgccc
     5221 ttgccacctc gccgctgcag gcggtacagg tccgccttcg tccgcttggc gtagccgccg
     5281 cgggtgatcg tcacgacgat gtcctcgtca gggaccaggt cctccatcga gaggtccccg
     5341 tcagccggga tgatctgcgt acgccgctcg tcgccgaact tgtcgacgat tgactgcagc
     5401 tcctcgctga cgatccgccg ctgccgctcc tcgctggcca ggatcgcctc gaggtcagcg
     5461 atggtctctt ccagctcggc gagtgcgtcg acgatccgct gccgctccat ggcggccaac
     5521 cgccgcagct gcatgtcgag gatcgcccgt gcctggacct cgtcgatgtc gaggaacgtc
     5581 atcagctcgt cacgtgcctc gtcggtggtc tgactccgcc ggatcagtgc gatgaccccg
     5641 tcgagggcgt ccagtgcctt gacgagaccg cgatacacgt gtgcctgttt ctcggcctcg
     5701 gacagccgat agcgggtgcg acgttggatg acgtcgatct ggtgggccac ccagttggag
     5761 atgaactggt cgatcgagag cgtgcgcggg acgtcgtcga ccagcgccag catgttgcag
     5821 gagaagttgt cctggagctg tgtgtgcttg tagaggttgt tgaggacgac ccgggcgacg
     5881 gcatcgcgct tgagaacgat gaccagccgc tgccccgtac gggaggagct gtcgtcgcgg
     5941 acgtcggcga tgccctgcac cttccccgaa tccgccagct cggcgatctt cagcgcgagg
     6001 ttgtcggggt tgaccatgta cgggagttgc gtgatgacaa ggcaggtgcg gttcttgctg
     6061 tcctcctcga tgtcgacgat cgcacgcatc gtcaccgaac cgcggccagt gcggtacgcc
     6121 tcctcgatgc cctgcgtgcc gacgatgagc gcacccaacg gaaagtccgg gcctttgacc
     6181 cgctcgatca gcgcgtcctg cagctcctga cgcgtggcgg tcgggttggc gagcgcccac
     6241 tgcacgccgt cgttgacctc acgcaggttg tgcggcggga tgttggtcgc cataccgact
     6301 gcgatgccgg ccgagccgtt gacgagcagg ttcgggaaac gggctggcag cagcaccggc
     6361 tcctggctgc ggccgtcgta gttgggccgg aagtcgacgg tgtcctcgtc gatgtcccgc
     6421 accatctcca tggccagcga cgccatcttg cattctgtgt tgtggctgac gaacccgttg
     6481 gtgatgaacg cgtggtcgtc ggtgtcgatc cggagcgaga aaacatcctg gatacctgcg
     6541 tcggtcacgc ccgccacctc ggcgtacagg aatcgcccgt cgaccagtgg ccacaccacc
     6601 tccgcacctt cggccgtgat ccggtccagg atttcaaccc ggtcccgttc ccaacgctcg
     6661 agtcgatcga tgttgtgctg ctgcagccag tactgcgact gcctgtcccg cgcagcatgc
     6721 cgacgcagaa actcggcgac aaacggcacc gcatcggcgg acgctgacga agccggttgc
     6781 tgcgagtcag ccatcgcctc gcggagcatc acgacgcgat caccagcttc gacttcttcg
     6841 agcaacttcc acagcagcgt cggaacccct gcgagtgagt tcagcgtgag gaccggatgg
     6901 ttcgtcgaac cggtgagctg gtggcccgag gttgttcgca gccgaagcgt gcgctgcgcc
     6961 ccgctgtgga agaacttcgt ggcgcgaacc gggtcgccgt ccttcccgat caccttgagg
     7021 tcgatctccg tctcagaacc gggagcggcg tcgggaacga tgtcgcggat ccgcatggtg
     7081 ccgtgctctg ccgtgcgaac ccgtgtttca cccgtcacgc agtagcgcat cgcggcgggg
     7141 cggtcgttgc cggctgagcc gaagtttccc tggccggaga cgagcgggtt gcgcatcacc
     7201 cacggctggc tcagccgcac caaggtgtcg tagatcgcgg tgtcgccgtg tgggtggtac
     7261 tgacccatga cgtcgccgac gacgcggctg cacttggaga acccgcgatc cggccggtag
     7321 cccccgtcgt acatcgcgta cagcactcgc cgatgcactg gcttgagccc gtcgcgtacg
     7381 tcgggaaggg cccggccgac gatgaccgcc atcgcgtagt cgatgtagga gcgctgcatc
     7441 tccgtctgca gctcgaccgg ctcgatacgc ccgcccggcg gcgcctccgg ggtggtcgtc
     7501 ccgtcgtcac tcaccggctg tcactccttc cattggtggg ctcgcggtca tgatgtggtc
     7561 ccttccggcg atcgagcttg tcgagatccc ggcttcgtga gctcgctggt ctcgacaggc
     7621 tcgaccggcg aagactcaga tgtcgaggaa acggacgtct tttgcgttgc gctggatgaa
     7681 cgaccgacgt tgctcgacgt cttcgcccat gaggatcgag aagatctcgt cggcatgcgc
     7741 ggcgtcgtcc aaggtcacct gtgacagcac ccggttgtcg gggtccatcg tggtttccca
     7801 gagctcctcg gcgttcatct caccgagccc tttgtagcgc tggatcgcgt tgtccttcgg
     7861 cagtttcttc ccttcggccc tgcctgcctc gatcaacgcg tcgcgctccc ggtcggagta
     7921 cacgaactcg tgctcgtgcg gattgttcca cctcaaccgg tacagcggcg gccgggcgag
     7981 gtagacgtgg ccgagctcga tcagcggctt catgaagcgg aacagcaacg tcagcaacag
     8041 ggtctggatg tggtgcccgt cgacgtcggc gtcggccatc atcacgatct tgtggtagcg
     8101 gagcttgtcg acgtcgaagt cttcctggat tccggtgccg agggcggaga tgatcgcctg
     8161 cacctcggtg ttggcgagca cccggtcgat gcgcgccttc tcgacgttga ggatcttgcc
     8221 gcggatcggg aggatcgcct ggatgcgtgg gtcgcggccc cctcgcgcgg aaccaccggc
     8281 cgagtcacct tcgacgatga agagctcgca ctcctccgga tcggtggact gacagtcgga
     8341 cagtttgccc ggcaacccac cgccaccgag caagcccttg cggttgcggg ccaggtcacg
     8401 cgccttccgg gccgccaccc gggccgtcgc ggccgcgatt cccttgcgga tgatgtcgcg
     8461 tccctcggtc gggttcttct cgaaccactc acccagccgg tcgttgacga cgcgctgggt
     8521 gaacgacttc gcctcggtgt taccgagctt ggtcttggtc tggccctcga actgcggctc
     8581 cgccagcttg accgagacga tggcggtcag tccttcacgg acgtcgtcac cggaaagcct
     8641 gtcctccttc ttcttgatca ggccccagct ttcggcccag ttgttgaccg ttgaggtcag
     8701 cgcggatcgg aacccctcct cgtgggtccc gccctcgtgg gtgttgatcg tgttggcgaa
     8761 ggtgtggacg gactcggtga agccggtgtt ccactgcatc gccacttcga ggctcatgcc
     8821 ggcctcgtcg ttggctgctt cgaacgcgat gatgctgcgg tgggccgggt ccttggtctt
     8881 gttgaggtgc ccgacgtagt cgatgagccc gtcctggtag cggaagcgtt gttcgatgcc
     8941 tccggaccca gtcacgtcgg tgcggatccg gtcggctcca ccgtcgatgg acgagtccac
     9001 tgtcgggtcg gcgaccgcct ccgcgatgcc gctggcatgc tccggccgct cgtcacggac
     9061 cacgatctcg aggcccttgt tgaggaaggc catctctcgg aagcgggatg cgagcgtctc
     9121 gaacgagtgg tcggtggtct cgaagatcgt ccgcgacggc caccaggtga ccgtcgtacc
     9181 ggtctcgtcg gtggcttcgt gtcgctgcag gtcaccgtca ggtaccccgt agctgaacga
     9241 ctgcgtccag cggtagccgt cgttcttcac ctcgacgaac agccgttcgc tgagggcgtt
     9301 gacgaccgag gaacccacgc cgtgcaggcc gccggacacc ttgtacccgc cgccgccgaa
     9361 cttgccgccg gcgtgcaact cggtgaggac cagggtgacg gccggaacgc cctcggtcgg
     9421 atggatcccc gtggggatgc cacgaccgtt gtccacgaca cggacaccgc caccggcgag
     9481 cagggtcacc tcgatcaggt cgcagtaccc ggccagcgcc tcgtcgacgg cgttgtcgac
     9541 gatctctgtg acgaggtgat gcagaccgcg ttcgccggtg gagccgatgt acatgccggg
     9601 acgcttgcgg acggcctcga gcccttcgag cagggtgatc tgcgatgcgt cgtacgccgg
     9661 gttgtcccca tccagctcgg gatcggccgg gggcaccggt acaccaggga gatcggcgag
     9721 ttcagtggcg gcgccactcg cggtctggtc ggtgcgggac gggctgacat cttggcggtc
     9781 gtcaccgaga ctcacgcttg cttcctccga agtctggtcg ggcctgcgtc gtggacggca
     9841 aggcgaccgt ggtccaccgg ccgaccaacc gaccgacgaa accccatcga cacgaaaccg
     9901 catcaactca gcacaaaggc cgcccggacg gatcgcggga cgacgtgtct cgccgtctcg
     9961 ctcgcagtcg tgtcggctgc gtccgcacgg tagtgcggcc ttgcttggga cgttcccagt
    10021 ataggcgatt ccgtgcccgg tcatggcatc cgcgcccact gcgtggaggg gtggatcgca
    10081 tcagaacacc cgatgcgcgg gcctacagtg ccagttgcgc gaaccagcgg cggcgaggat
    10141 atgcccccac atgcccgacg gggtcgtaga tcggctccga gccgctcgtc gacgggtgca
    10201 gatcagccgt aggtgtcccg gggtccgcga ccaccacgga ctgtgcgcgg ccctcgtcgc
    10261 cacgacggcg atgccggccc gacgacctcg atccgtacga cggtcccgtc accgagctcc
    10321 tcgttcagcc ggcgtacgac ggtcggtgcc agcagcttca gctgggtcgc ccaggc
//
LOCUS       DATBFZ010000215         1750 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_4076971, whole genome shotgun sequence.
ACCESSION   DATBFZ010000215 DATBFZ010000000
VERSION     DATBFZ010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1750
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4076971"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1745)
                     /locus_tag="VH419_06515"
     CDS             complement(<1..1745)
                     /locus_tag="VH419_06515"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY7043303.1"
                     /translation="MTSVNLVANTNLKTVDEFRKLVVKEQGGVVVRLGEIADVVLGAE
                     NYEQDVRFNGETGTFMGIWVLPTANSLDVIQRVRAAIPGIRTQLPAGMKLGVPYDSTE
                     YIQDAIDEVLTTLTETLLIVVVVIFLFLGSFRSVLIPIVAIPISLVGAVFLMLVAGFT
                     INLLTLLAIVLSVGLVVDDAIVMVENIERHLHSGKQPVQAATDAARELVGPIIAMTIT
                     LAAVYTPVGIQGGLTGALFREFAFTLAGAVIVSGIVALTLSPMMGSKLLRAGDTERGF
                     AGWVNRRFETVRARYVRTLAGTLRYRPVVLVLWVIVALLVVPFYMFSQRELAPNEDQG
                     VVFSILQTSANSTLDQTKLFANEVADAYRSMPETTSIFQITLPNGGFGGMVTKPWSAR
                     TRTTQEILVNLTGPLSKIPGVRVIPLTPPALPGGGDFPVDFVIESPVEPQQLGEIAGQ
                     IVQKAMASGMFIFADADLKFDQPQTEVVFDRDKLRAQGVDLSQAGQDLSTMLGGDYVN
                     RFSIQGRSYKVIPQVARTERLTPDQLSEMYVTGADGKLVPLSTFATLRTTSEPRELKK
                     FQQLNAVRIQGVIPPP"
BASE COUNT          307 a          587 c          568 g          288 t
ORIGIN      
        1 ggcggtggaa tcacgccctg aatccgaacc gcgttgagct gctggaactt cttcagctcg
       61 cgcggctcgg acgtcgtgcg cagcgtcgcg aatgtcgaga gcggcaccag cttcccgtcc
      121 gcgccggtga cgtacatctc cgacagctga tcgggcgtca gccgctcggt gcgggctacc
      181 tgcgggatca ccttgtagct ccggccctgg atgctgaagc ggttcacgta atcgccgccc
      241 agcatcgtcg acagatcctg gccggcctgg ctcaggtcca ccccctgcgc ccggagcttg
      301 tcgcgatcga acaccacctc ggtctggggc tggtcgaact tcaagtcggc gtcggcgaag
      361 atgaacatcc cgcttgccat cgccttctgc acgatctgcc cggcgatctc gccgagctgc
      421 tgcggttcga ccggcgattc gatgacgaag tcgacgggga agtcgccgcc gccgggaagc
      481 gcgggcggag tcagcggaat tacccgcacg ccgggaatct tcgacagcgg acccgtcagg
      541 ttgaccagga tctcctgcgt cgtcctggtg cgcgcgctcc agggcttcgt caccatgccg
      601 ccgaagccgc cgttgggcag cgtgatctgg aagatgctcg tggtctcggg catcgaccga
      661 tacgcgtcgg ccacttcgtt cgcaaagagc ttcgtctgat cgagcgtgga gttggccgaa
      721 gtctgcagga tcgagaacac cacgccctga tcctcgttcg gcgccagctc gcgctgcgag
      781 aacatgtaga agggcaccac gagcagggcg acgatgaccc acagcaccag caccaccggg
      841 cgatagcgca gcgtgccggc gagcgtccgc acgtaacgcg cgcgcacggt ctcgaaccgg
      901 cggttgaccc atccggcaaa gccacgctcg gtgtcgcccg cgcggagcag cttcgagccc
      961 atcatcggtg acagcgtgag cgcgacgatg cccgacacga tcacggcccc ggcgagcgtg
     1021 aacgcgaact cccggaacag cgcgcccgtg agcccgccct ggatgccgac cggcgtatac
     1081 acggcggcca gcgtgatcgt catggcgatg atcggtccga ccagctcgcg cgccgcatcg
     1141 gtggcggcct ggaccggctg cttcccgctg tgcaggtgtc gctcgatgtt ttccaccatc
     1201 acgatcgcgt cgtccacgac gaggcccacc gagagcacga tggccagcag cgtcaggagg
     1261 ttgatcgtga agccggcgac cagcatgagg aacacggcgc cgacgagcga gatcgggatc
     1321 gccacgatcg ggatcagcac cgatcggaag gatccgagga acaggaagat gacgacaacg
     1381 acgatgagga gcgtctcggt gagcgtcgtg agcacctcgt cgatcgcgtc ctggatgtac
     1441 tccgtggaat cgtagggcac gccgagcttc attccagcgg ggagctgcgt ccggataccc
     1501 ggtatcgccg cgcgcacccg ctggatgacg tcgagcgagt tggcggtcgg cagcacccag
     1561 atgcccatga acgtcccggt ctcgccgttg aatcggacgt cctgctcgta gttctcggcg
     1621 cccagcacga cgtcggcgat ctcgcccagg cggaccacca cgccgccttg ttccttcacc
     1681 accagcttgc ggaactcgtc gaccgtcttg agattcgtgt tcgcgaccag gttgaccgac
     1741 gtcatcgtcc
//
LOCUS       DATBFZ010000216         1627 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_2110490, whole genome shotgun sequence.
ACCESSION   DATBFZ010000216 DATBFZ010000000
VERSION     DATBFZ010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2110490"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..1319)
                     /locus_tag="VH419_06520"
     CDS             complement(57..1319)
                     /locus_tag="VH419_06520"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HEY7043304.1"
                     /translation="MTFVLPTGLGDGLEYVQRSGRWADLERLRSGNPMYDATIEEIDG
                     RRIRVGNKWLADFASCNYLGFDLEPEIMAAIEPEVKRWGTHPSWSRLLGNPSLYPQIE
                     ERLTDLLDAPDTLVMPTITHIHMSVLPILAGNGFIFLDSQAHKTIYDGSMYARGLGAT
                     VQRFKANDHEHLAQMLRSVPGGVSKIVAMDGVNSMTGNAPDLAAFAKVCRENGALLYV
                     DDAHGFGVIGERAANETSPYGIKGNAIVKYCGESYDNIVLVGGFSKSYSSLLAFLALP
                     TWLKNHLKVSAPPYLYSGPAPTASLATVMAGFDVNEKRGDAIRTDLYRKTMKVLDHVR
                     GLGVFTPNTGTTPVIELPLAEGEDIDAVGKLLWERGIYVTLAAYPLVPRDQVGFRAQV
                     TAANSDEEIDELNAVITELAEAGKLRRR"
     gene            complement(1343..>1627)
                     /locus_tag="VH419_06525"
     CDS             complement(1343..>1627)
                     /locus_tag="VH419_06525"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY7043305.1"
                     /translation="YSSLSYLSQMPVDVLKIDKSFIDDITHSNQQMALVETIVSLART
                     LDLAVVAEGIELDEHRQALVGMGCPYGQGYLFSKPVTADEIGQWLIAYTH"
BASE COUNT          245 a          530 c          564 g          288 t
ORIGIN      
        1 ggtccacagc agccggaact gccgatggcg aagcggcgcg agtggaccgg cagccttcat
       61 cgacgacgca gcttcccggc ctcggcgagc tcggtgatga cggcgttgag ctcgtcgatc
      121 tcctcgtcgc tgttggccgc ggtgacctgc gcccggaagc ccacctggtc gcgcgggacc
      181 agcgggtacg cggccagcgt cacgtagatg ccgcgctccc agagcagctt gccgaccgcg
      241 tcgatgtcct cgccctcggc cagcggcagc tcgatcaccg gcgtggtgcc ggtgttcggg
      301 gtgaacacac ccaggccgcg tacgtggtcc agcaccttca tggtcttgcg gtacaggtcg
      361 gtgcggatcg cgtcgccgcg cttctcgttg acgtcgaagc cggccatcac cgtcgccagc
      421 gaggcggtcg gcgccgggcc cgagtacagg tacggcgggg ccgagacctt gaggtggttc
      481 ttcagccagg tgggcagggc caggaaggcg agcagcgacg agtacgactt cgagaagccg
      541 ccgacgagca cgatgttgtc gtagctctcg ccgcagtact tgacgatcgc gttgcccttg
      601 atcccgtacg gggaggtctc gttggccgcc cgctcaccga tcacgccgaa cccgtgcgcg
      661 tcgtcgacgt agagcagggc gccgttctcc cggcacacct tggcgaacgc ggccaggtcg
      721 ggcgcgttgc cggtcatgct gttcacgccg tccatcgcga cgatcttcga gacgccgccg
      781 ggcaccgacc gcagcatctg ggcgaggtgc tcgtggtcgt tggccttgaa ccgctgcacg
      841 gtggcgccga ggccgcgggc gtacatgctg ccgtcgtaga tcgtcttgtg ggcctgcgag
      901 tccaggaaga tgaagccgtt gccggccagg atcggcagca ccgacatgtg tatgtgcgtg
      961 atggtcggca tcaccagcgt gtccggcgcg tcgagcaggt cggtgagccg ctcctcgatc
     1021 tgcgggtaca gcgacgggtt gccgagcagg cgggaccagc tcgggtgggt gccccagcgt
     1081 ttcacctcgg gctcgatcgc cgccatgatc tccggctcca ggtcgaagcc gaggtagttg
     1141 caggacgcga agtcggcgag ccacttgttg ccgacccgga tgcgccggcc gtcgatctcc
     1201 tcgatcgtgg cgtcgtacat cgggttgccg ctgcgcaggc gctccaggtc tgcccagcgc
     1261 cccgagcgct gcacgtactc gagcccgtcg ccgaggccag tggggagaac gaaggtcatc
     1321 tcattccttt ccatggggac catcaatgcg tgtacgcgat cagccactgg ccgatctcgt
     1381 cggcggttac cggtttgctg aacaggtagc cctggccgta cgggcagccc attccgacga
     1441 gcgcctgccg gtgctcgtcc aactcgatgc cctcggccac cacggccagg tcgagcgtgc
     1501 gggccaggct cacgatggtc tcgacgagag ccatctgctg gttgctgtgg gtgatgtcgt
     1561 cgatgaacga cttgtcgatc ttgagaacgt ccaccggcat ctggctcagg tagctgagcg
     1621 acgagta
//
LOCUS       DATBFZ010000217        12945 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_1451193, whole genome shotgun sequence.
ACCESSION   DATBFZ010000217 DATBFZ010000000
VERSION     DATBFZ010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 12945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12945
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1451193"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="VH419_06530"
     CDS             <1..467
                     /locus_tag="VH419_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012918232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEY7043306.1"
                     /translation="TRPANQEAAGLVTLEDLVRQALRMRPDRLVVGEVRGAEVVDLMA
                     AMNTGHDGGCSTVHANSAADLPARFEALGVAAGLPRPAVHAQLASALDVVLHLRRDRT
                     GQRRLAEVGVLQRSESGLVTVCPAVRFGVGGAPVLADGADRLDTLIATQCLL"
     gene            480..1280
                     /locus_tag="VH419_06535"
     CDS             480..1280
                     /locus_tag="VH419_06535"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEY7043307.1"
                     /translation="MNGQATAATTAAVLAALAVALASASAPLPRTVVGAPRSVSSRWV
                     APAALVTCLLAASNLATGIRLVLLVVAASVLTFVVRRLLERIRRRHRRQATELAVIEF
                     CDALAAELAAGVATGRAVELAAAVNPEWRAVVVTGRLGGDVAAALRDQAGRPGAHGLN
                     AVAAAWEVSVGSGAALADVLDKTAAALRDEQEARVEVAAALAVPRATARMLAVLPLFG
                     LGLGMSMGADPLGFLLGSTFGLAVLMCGLLLALIGVVWVERLADGAEQ"
     gene            1264..2064
                     /locus_tag="VH419_06540"
     CDS             1264..2064
                     /locus_tag="VH419_06540"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEY7043308.1"
                     /translation="MAPSNEPRNEPGKGEVDVVIETTMAAGAAAAAVWVATPREAAGR
                     IHHMLGAGHRDHGANRRDSRRSLDDQRVRALVCVLACSALACVFVGGVGALAAVPAGL
                     TLSWWLGRVEPRRVARDRAAFRRDLPLAIELLAACADAGLPLEQALGPVAGAVRGPLG
                     DRLLLLSARLALGASPIDEWGRMGADPELAGLGNAVIRAHRSGAPVAATLGRLAGDSR
                     RTRRSRQQAAARAVGVKVAAPLAACFLPAFMLIGVVPTVVTGFAQLGL"
     gene            2212..2463
                     /locus_tag="VH419_06545"
     CDS             2212..2463
                     /locus_tag="VH419_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017933266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4244 domain-containing protein"
                     /protein_id="HEY7043309.1"
                     /translation="MRPGTSSAPSGRTLASASNTGTAGRVDAVLPRRGESGMSTAEYA
                     VGTVGACGFAGLLYQLLTSDFGRNLLSGLFEKVLDLLPF"
     gene            2523..2900
                     /locus_tag="VH419_06550"
     CDS             2523..2900
                     /locus_tag="VH419_06550"
                     /inference="COORDINATES: protein motif:HMM:NF041390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE family type IV pilus minor pilin"
                     /protein_id="HEY7043310.1"
                     /translation="MGAAERSAHGSVTAEAAIVLPIMAAFALTLIWMISVGIAQIQAV
                     DAARDAAREIARGGDVDAATARARVTAPADARVDIDRSAGEAVVTVSFTAKPPRWLFV
                     PLPPVGISASSSVEVERDDAREP"
     gene            2884..3237
                     /locus_tag="VH419_06555"
     CDS             2884..3237
                     /locus_tag="VH419_06555"
                     /inference="COORDINATES: protein motif:HMM:TIGR03816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rv3654c family TadE-like protein"
                     /protein_id="HEY7043311.1"
                     /translation="MPANRESTDGGYATVWAVGWMACSLTVALCVLLATVAVAAQHHL
                     DGSADLVALAAAGAAQHGVDPCASARKAARANGVALIGCLVDRDDVTVHVSDVVRFPL
                     GLDTRIEGWARAGPA"
     gene            complement(3241..3618)
                     /locus_tag="VH419_06560"
     CDS             complement(3241..3618)
                     /locus_tag="VH419_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043312.1"
                     /translation="MIILGLLILLGVAAVTVVALVRGGADVSIDVGSFTVNTDAAGMF
                     IAGGVSLLLAVVGLWLLNRGMKRSRRRRREMHELRDRAARNDAASRREQPAARSETGP
                     DHTTAAGRSTDADDHFDTTPRER"
     gene            complement(3738..6047)
                     /locus_tag="VH419_06565"
     CDS             complement(3738..6047)
                     /locus_tag="VH419_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017933262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY7043313.1"
                     /translation="MFDPRAVALRLAAGSTRRDRLTHLEILPAASAVTAAWPTWTHPT
                     LVDAYRVRGVSTLWSHQAEAAELAWSGRHVVVATGTASGKSLAYLLPTLSAIARSVER
                     PGARGDTVLYLSPTKALAHDQLASMAGLDVPGVRAAAYDGDSSQAERDWARNHANYLL
                     TNPDMLHRTMLPTHARWAVFWGSLRYVVVDECHHYRGVFGAHVAQILRRLRRVAGHYG
                     ATPTFVLASATAADPEVTACRLVGTDVTAVDVDGSPRGQTVVALWEPPLTSLRGEQGA
                     PVRRSATAETADLVADLVADGVRTLAFIRSRRGAETVALQAQAKLHEVDVDLAKRVAP
                     YRGGYLPEDRRELEQRFKSGDLLAVSATNALELGIDISGLDAVVMAGFPGTRASMWQQ
                     IGRSGREGQGGLGVLVARDEPLDTYLVTHPEALLGSPVEASVFDPGNPYVVAPHLCAA
                     AQEIPLTEEDAQVFAGDVEAVVASLLEAGYLRRRARGWFWTRRDRAANLADIRSTGGN
                     PVRIVEGSTGRLVGTVDASSAHATVHRGAVYIHLGQSFLVESFDHAEGVAVVEAGDPE
                     YTTTARELTDIRVLDEDLAASWGAATLTFGAVEVSSQVVSFLKKRLATGELLGEEPLD
                     LPPRHLQTKAVWWTLTPEQIEASGVHPRDVPGAAHAAEHASIGLLPLFATCDRWDIGG
                     VSTALHDDTGLLTVFVYDGHPGGAGFAERGFEAAADWLTATREAIAACACDEGCPSCV
                     QSPKCGNGNRPLDKRGAIWLLDALLAGHD"
     gene            6176..6511
                     /locus_tag="VH419_06570"
     CDS             6176..6511
                     /locus_tag="VH419_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012918248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEY7043314.1"
                     /translation="MDLSLDNRADGERMVVDVGGEIDVYTAPKLRERLVELINSGHYN
                     LIVNLEGVDFLDSTGLGVLVGALKRVRAHEGSLLLVCTQERLLKIFRITGLAKVFPIY
                     DSVGAAIKA"
     gene            6631..8988
                     /locus_tag="VH419_06575"
     CDS             6631..8988
                     /locus_tag="VH419_06575"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020577090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HEY7043315.1"
                     /translation="MSRLSLAQSGADVSLSGGNITLVVIVALIALVALGMSVMFRQEV
                     LAAPEGTDNMKSISAAVQEGANAYLTRQFRTLAIFAVVAFALLFVLPGDASIRIGRSL
                     FFLVGAGFSASIGYLGMSLAVRANTRMAAAARDAGRDPAMKIAFRTGGVVGMATVGLG
                     LLGASVVVLIYKEDAPSVLEGFGFGAALLAMFMRVGGGIFTKAADVGADLVGKVEQNI
                     PEDDPRNAATIADNVGDNVGDCAGMAADLFESYAVTLVAALILGKAAFGSEGLVFPLI
                     VPAIGALTAILGIYLCKPRAGENGLTTINRAFYISAAVSAVLCVLAAFIYLPTSFDDL
                     HGGGGVSLVEQFLQTSPGVDGNPALIASIAVIIGIVLASLILTLTGYFTGTEHRPVRD
                     VGKTSLTGPATVILSGVSVGFESTVYTALVIGAAVFGAYLLGSGAILVSLFAVALAGC
                     GLLTTVGVIVAMDTFGPVSDNAQGIAEMSGDVHGEGAAILTELDAVGNTTKAITKGIA
                     IATAVLAATALFGSFTDTVGSEAEKLTGAGDLSAGDVLDLLGVLNVADPKNLVGLLIG
                     ASVVFLFAGLAINAVGRAAGAVVFEVRRQFREIPGIMEGTGRPEYGRVVDICTRDSLR
                     ELATPGLLAVLAPIAVGFGLGVGPLGCYLAGAIGTGTLMAVFLANSGGAWDNSKKLVE
                     DGYHGGKGSPAHEATIIGDTVGDPFKDTAGPAINPLIKVMNLVSLLIAAAVVKMTYGD
                     DSNTALRIVIAVVAALAIATAVYISKRRPVAIGDADTEPAESTSV"
     gene            9057..10226
                     /locus_tag="VH419_06580"
     CDS             9057..10226
                     /locus_tag="VH419_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043316.1"
                     /translation="MEEDRLSRTVFLHVGVAKTGTTYLQRLLFANRELLRSAGVLYPG
                     QRPEAHFLASMDLRDAGLGGHKYPAAAGRWAKLANTVNCFDGTAVISHETLSRARRPA
                     IQRAIEAFETDDIRVVLTTRDLGRQIPAVWQENVKNRNPQTYADFLHGVLVGVPPAKP
                     GPGKPDLVRLVSFWWPQNLPKMVGRWAPVVGTDKVVLVTVPPLGSPRDELWKRFATAV
                     ELPVLDYEFDVVGENVSLGAAEAELMRRVIAYVPAEVDWPRWKNRVKRGLGERTLASM
                     GAGGRILVPKQWHDVLADISEQMIADLAASGVRVVGDLDDLRPRFDQEDGLLPDDVSD
                     AELLECASAALAQMASAPPEHVRSVHNAPTQRPENGDPQRTRGEANVQKEKPRRG"
     gene            10219..11334
                     /locus_tag="VH419_06585"
     CDS             10219..11334
                     /locus_tag="VH419_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043317.1"
                     /translation="MAERVYIHIGAPKTGTTSLQQALYANRERMEEVGVLYAPERHDA
                     HHSAVLDLRDAWQRENASHLRGAWKQVVRQVRQWDGPTAVLSSELFVYGSKQEAARAL
                     SAFGDAEVHVIYTARDLVRQVPAVWQERMKNQYTMPYGRFVQDVIGPARSPMARGFWS
                     AQDAPEALKRWSKGLPSSHVHVVTAPPPGSSPALLWDRFLSVLGLEGAAYPADVSVLN
                     PSLGAVEAEVLRRLNRRHGQDLGPGAYRMIVRNGLFDVLDHVVPDKIKITLRSDEHNA
                     LVAKGRQIADGIRRGGYDVVGDLADLVPPSRTPEDDADAARRPDDVRAAEMVNVLVDV
                     VYALLEERRALKDSHVPRARRRRRRSLELRASGDERS"
     gene            complement(11349..12548)
                     /locus_tag="VH419_06590"
     CDS             complement(11349..12548)
                     /locus_tag="VH419_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043318.1"
                     /translation="MPTPDPAGPPGKRVYFHIGAPKTGTTYLQHVLLQNKEALAKHGV
                     LYPYDDHAQSFRSMQDFKGLGWGGQRARAFKGEWKKVADRTVAWRGETIVISNELLGG
                     AAVERISAGVASVAPADVHVIFSARDLARQLVSDWQEHVKHKHEVTLEKFVDDLITLG
                     LDAPKPFGELFWGMHDAAHVLRRWAVFVPKQNIHVLTLPQPGAPRATLWHRFCEVIGV
                     QPDAYDSVTTSSNPSMGVAETELVRRMNGSVQGMPAENYDPLVRLLLAEHILGGQPPR
                     LTLPPGRLDWVMKRSRRLIDELTEAGYPVVGDLEDLMPNPADHATYVSPTELTDADLG
                     PAAIRAATGLLQHAGRQRRTIVELQKAGTDTGARSRPLRDRFAGGYRRLRSRVGKLLR
                     RLGLLHR"
     gene            12643..>12945
                     /locus_tag="VH419_06595"
     CDS             12643..>12945
                     /locus_tag="VH419_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043319.1"
                     /translation="MTDSAAETAHVASGWRVVQRTIIRPDQELDIRPLYVTGLSGFGG
                     AASTARQSGEEIPDVERQIRGKGIPANLYDDSADEGVEALGAFGTITEAGAVANAEH"
BASE COUNT         1902 a         4398 c         4547 g         2098 t
ORIGIN      
        1 agaccaggcc ggcgaaccag gaggccgcgg gcctggtcac cctggaggac ctggtgcgcc
       61 aggcgctgcg catgcggccg gaccggctcg tcgtgggtga ggtccggggc gccgaggtgg
      121 tcgacctgat ggccgcgatg aacaccggtc acgacggcgg ctgcagcacg gtgcacgcga
      181 actccgcggc tgacctgccg gcacgtttcg aggcgctcgg ggtcgcggcc gggctgccgc
      241 gcccggccgt ccacgcccag ctggcgtcag cgctcgacgt ggttctccac ctgcgtcgcg
      301 accgcacggg ccagcggcgg ctggccgagg tcggtgtgct ccagcgttcg gagtctgggc
      361 tggtgacggt gtgtccggcg gtccggttcg gtgttggtgg cgctccggtg ttggccgacg
      421 gagccgatcg tctcgacacg ctgatcgcca cacagtgtct cttgtgagca gtgtcatttg
      481 tgaatgggca ggcgacagct gcgacgactg cggcggtgct cgcggcgctg gccgtggcct
      541 tggcgagcgc atcggcgccg ctccctcgca ctgttgtagg ggcaccgcgc agtgtctcga
      601 gtcgatgggt tgctcccgcc gcgctggtca cgtgcctgtt ggccgcgtcg aacctcgcca
      661 cgggcatccg cctggtcctg ctggtggtgg cagcaagcgt tctcaccttc gtcgtacgcc
      721 gattgctcga gcgcatccgg cggcgtcatc ggcggcaggc caccgagctg gcggtgatcg
      781 agttctgcga cgcgctcgcc gccgaactgg ccgccggggt ggcgaccgga cgcgcagtgg
      841 agctggctgc tgccgtcaac cccgagtggc gggctgtcgt cgtgaccggc cgactcggcg
      901 gagacgttgc agccgctctg cgagaccagg ccgggcgccc aggcgcgcac ggtctcaacg
      961 cggtcgccgc cgcatgggag gtgtcggtgg gctcaggagc cgcactggcc gatgtgctcg
     1021 acaagaccgc agcggcgctt cgtgacgagc aggaggcccg cgtcgaggtc gccgctgcac
     1081 tggcggtgcc gcgcgccacg gccaggatgt tggccgtgct tccgttgttc ggactcgggc
     1141 tcgggatgtc gatgggtgcc gatccgctgg gcttcctgct ggggtccacg ttcgggctcg
     1201 ccgtgctcat gtgcgggctg ctcctggcgc tgatcggtgt ggtgtgggtg gagcgactcg
     1261 cggatggcgc cgagcaatga gccccgcaat gagccgggca agggcgaggt cgatgtcgtg
     1321 atcgagacca cgatggccgc cggtgcggcc gctgcggctg tgtgggtcgc cacgccccgc
     1381 gaagcagccg gccgcatcca ccacatgctg ggcgccggtc acagggacca tggggcgaac
     1441 cgccgtgaca gtcgccgttc gctcgacgat caacgcgtgc gcgccctcgt gtgcgtgttg
     1501 gcctgctcgg cgttggcctg cgtgttcgtc ggcggtgtcg gtgcgctggc agccgtccct
     1561 gcggggctga cgctgtcgtg gtggctgggc cgggtggagc ctcggcgtgt tgctcgtgat
     1621 cgcgccgcct tccggcgcga cctgccgctg gccatcgagt tgctcgccgc gtgtgcggac
     1681 gccggactcc ccctcgagca ggcgctcggt ccggtggccg gggcagtgcg tggaccgctg
     1741 ggtgatcgcc tgttgctgtt gtcggcacgc ctcgcactcg gggcatcgcc tatcgacgag
     1801 tgggggcgga tgggcgccga tccggagttg gcgggtctgg gcaacgccgt catccgggcg
     1861 catcgctccg gcgcgccggt cgcggccacg ttgggacgac tcgccgggga cagccggcga
     1921 acgcgtcgga gcagacagca ggcggcagct agagccgtgg gcgtgaaagt ggccgctccg
     1981 ttggcagcct gcttcctgcc ggcattcatg ctcatcggag tcgtgccgac cgttgtcacc
     2041 gggttcgcgc aacttggcct gtgactgtcc ccaggtcacg tcgtcgacgg catccgtcaa
     2101 caactgtcga cagcagccac cggcgtcagt cgaacgggcg gttggctggg cggcacccat
     2161 ccacacgtcg tccggccccg ccggagacat cgtcatgaag ggacaccaca catgagaccc
     2221 gggacatcca gcgcccccag cggacggaca ctggccagtg ccagcaacac cgggaccgcc
     2281 ggacgcgtcg acgccgtgct tccccggcgc ggtgagagtg gcatgagcac cgccgagtac
     2341 gccgtcggga ccgtcggcgc gtgcgggttc gccgggctgc tctaccagct gctcacctcc
     2401 gacttcggtc gcaacctgct cagtggtctg ttcgagaagg tcttggacct gctgccgttc
     2461 tgaacggtgc gggcggggcg cgctcgatgt cccgcccgca tcgaccgacc cgaggaggtg
     2521 tgatgggcgc ggcagagcgg tctgcgcacg gaagcgtgac ggcggaggcc gcgatcgtgc
     2581 tgccgatcat ggcggcgttc gcgttgacgc tgatctggat gatctccgtc ggcatcgcac
     2641 agatccaggc tgtcgatgcg gcgcgggacg ccgcgcgtga gatcgcccgg ggcggcgacg
     2701 tcgacgccgc caccgccaga gcacgcgtca ccgctccagc ggatgcgcgc gtcgacatcg
     2761 accggtccgc cggcgaggca gtggtcacgg tctcgttcac ggcgaagcct ccacggtggc
     2821 tgttcgtgcc gctgccaccg gtcgggatca gcgcgtcgtc cagtgtcgaa gtggagcgtg
     2881 atgatgcccg cgaaccgtga gtccaccgac ggcggctatg cgaccgtgtg ggcggtcggc
     2941 tggatggcgt gcagcctcac agtggcgctg tgcgtgttgc tggccaccgt cgccgtcgca
     3001 gctcaacacc atctcgacgg gtcagccgac ctcgtcgcgc tggctgcggc aggtgcggct
     3061 cagcacggtg tcgatccctg cgcgtccgcc cgaaaggcgg cccgtgccaa cggtgtcgcc
     3121 ctgatcgggt gtctggtgga ccgtgacgac gtcactgtcc atgtcagcga cgtcgtgcgg
     3181 tttccgttgg gcctggacac ccggatcgag gggtgggccc gtgcggggcc ggcctgagcc
     3241 tcagcgctcg cgaggtgtcg tgtcgaaatg atcgtcagcg tcggtcgagc gtcccgctgc
     3301 ggtggtgtgg tccgggcccg tctccgaacg tgccgccggt tgctcacgtc gtgatgcggc
     3361 gtcgttgcgt gcggcccggt cacgcagttc gtgcatctcc cggcgccgac gtcgagaccg
     3421 tttcatcccc ctgttcagta gccacaggcc caccaccgcc agcaacaggc tcaccccgcc
     3481 ggcgatgaac atgccggcgg cgtcggtgtt gaccgtgaac gacccgacgt cgatcgagac
     3541 gtcggcacca ccgcggacca gtgcgacgac ggtgacagca gcgacgccga ggagaatcag
     3601 tagtccgagg atgatcatgg gtacgtctcc tcccgaccgc acgggatcgt ccatcccccc
     3661 aatacccctg ctggtgagat acaacccact gtgtcagatc ggggtcagat cggggtcaga
     3721 tcggggtcgg agcggggtca gtcgtgcccc gccagcaatg catcgagcaa ccagatcgcg
     3781 ccccgcttgt cgagcgggcg gttcccgttc ccgcacttgg gtgactgcac gcacgacggg
     3841 cacccctcgt cgcaggcaca cgccgcgatc gcctcacggg ttgccgtgag ccagtccgcc
     3901 gcagcctcga accctcgctc cgcgaagccc gcaccgcccg gatgaccgtc gtacacgaag
     3961 accgtgagca gcccggtgtc gtcgtgcagc gccgtcgaca ccccgccgat gtcccagcga
     4021 tcgcaggtcg cgaagagcgg cagcagcccg atcgacgcat gctcggcggc atgcgccgcc
     4081 cccggtacgt cgcgtggatg gacgccgctg gcctcgatct gctcaggcgt gagggtccac
     4141 cacacagcct tggtctggag gtgtcgtggc ggcaggtcca gcggttcctc gccgagcagc
     4201 tcgccggtcg cgaggcgctt cttgagaaac gacacgacct gcgacgagac ctccaccgct
     4261 ccgaacgtga gggtggcggc accccatgac gccgcgagat cctcgtcgag gacccggatg
     4321 tcggtgagtt cgcgtgcggt cgtcgtgtac tccggatcgc cggcctcgac gacagcgaca
     4381 ccctcggcgt ggtcgaacga ctcgaccagg aagctctgtc cgaggtggat gtacaccgcg
     4441 ccccgatgca cggtcgcatg tgccgacgac gcgtcaacgg tgccgacgag ccgtcccgtg
     4501 gaaccctcga cgatcctgac cgggttgcct ccggtcgacc ggatgtcggc caggttcgcg
     4561 gcacggtcac gtcgtgtcca gaaccagccg cgcgcgcgcc ggcgcaggta acccgcctcc
     4621 agcagcgacg ccacgacggc ttcgacgtcg ccggcgaaca cctgtgcgtc ctcttcggtc
     4681 aatgggatct cctgcgccgc ggcgcacagg tgcggggcga cgacgtaggg gttgcccggg
     4741 tcgaagacgc tcgcttcgac cggcgatccg agcagcgctt cggggtgcgt gacgagatag
     4801 gtgtccagcg gctcgtcgcg ggcgacgagg acacccagcc cgccctgtcc ctcccgaccc
     4861 gatcgaccga tctgttgcca catcgacgca cgggtgcccg gaaagccggc catcacgacc
     4921 gcgtccagac cgctgatgtc gatgcccagc tcgagggcat tggtcgccga gacagccagc
     4981 aggtcaccgc tcttgaaccg ttgctccagc tcgcggcgat cttcgggcag gtacccgccg
     5041 cggtacggtg cgacgcgctt cgccaggtcg acgtcgacct cgtgcagctt cgcctgcgcc
     5101 tgcaacgcga cggtctcggc gccgcgacgg ctgcgtatga acgccagcgt ccgtacgccg
     5161 tcagcgacca ggtcggcgac gaggtcagcc gtctccgccg tggctgagcg ccggaccgga
     5221 gctccctgct caccgcgaag cgaggtgagc ggaggctccc aaagggccac gacggtctgt
     5281 ccgcgcggcg acccgtcgac gtcgaccgcg gtgacgtcgg tgccgacaag ccggcaggcg
     5341 gtcacctccg ggtcggcggc ggtcgccgag gccagcacga acgtcggagt ggcaccgtag
     5401 tgcccggcca cccgacgcag ccggcgcagg atctgcgcga cgtgcgcccc gaacacgccg
     5461 cggtagtggt ggcactcgtc gaccacgacg tagcgcaacg agccccagaa caccgcccat
     5521 cgcgcatgcg tcggcagcat cgtccggtgc agcatgtcgg ggttggtcag caggtagttg
     5581 gcatggttgc gcgcccagtc tcgctcagcc tggctggagt caccgtcgta cgccgctgcg
     5641 cgcacgccgg gtacgtcgag cccggccatc gaggcgagct ggtcatgagc gagcgccttc
     5701 gtcggcgaca ggtacagcac ggtgtcgccg cgggcaccgg gtcgctcgac actgcgcgcg
     5761 atcgctgaca gcgtcggcag caggtaggcc agcgacttcc cggaggcggt gccggtcgcc
     5821 accacgacgt gccgccccga ccaggcgagc tccgcggcct cggcctgatg gctccagagc
     5881 gttgacacgc cccggacgcg gtaggcatcg accagtgtcg gatgggtcca cgtcggccaa
     5941 gcagccgtga ccgccgaggc ggccgggagg atctccaggt gcgtgagacg gtcgcgccgg
     6001 gtcgacccgg cggccagccg caaggccact gcgcgaggat cgaacacccg cggtcccctc
     6061 accacgatcc gacatcctgt catcgccgca cccccggttt cgcagtcgtg gttgaatgtg
     6121 ctgcatcgag ggcctgcagc agctcgatcc gcgttggcaa ccaaggaggc ggcacgtgga
     6181 cctgtccctg gacaacaggg cggacggcga acgcatggtc gtcgacgtcg gcggtgagat
     6241 cgacgtctac acggcgccga agttgcgtga gcggctggtc gagctgatca actccggcca
     6301 ctacaacctg atcgtcaacc tcgaaggtgt cgacttcctc gactccaccg gactcggcgt
     6361 actcgtgggt gcgctcaagc gggtccgcgc ccacgaaggg tcgctgctgc tggtgtgcac
     6421 gcaggagcgg ctgctgaaga tcttccggat caccggcctg gcgaaggtct tccccatcta
     6481 cgactccgtg ggggcggcca tcaaggcctg agctgccgca aacggcgcgg ctgcgacgtc
     6541 gtggcgccga cccggtcgtg tgcggccgac aacgctggct tagactccgg cggacttgtc
     6601 tcgcgagcgg catcaccatg gaggtagcgc atgtcccggc tcagcctcgc ccaatccggg
     6661 gcggatgtgt ccctctccgg gggcaacatc accctcgtcg tcatcgtggc cttgatcgcc
     6721 ctggtggcgt tgggcatgag cgtgatgttc cggcaggaag tgctcgccgc gcccgagggc
     6781 accgacaaca tgaagtccat cagcgcagcg gtgcaggaag gtgccaacgc ctacctcacc
     6841 cgtcagttca ggacgctggc gatcttcgcg gtggtcgcgt tcgccttgct cttcgtgctt
     6901 ccgggcgacg cgagcatccg gatcgggcgg tcgctgttct tcctggtggg cgccgggttc
     6961 tcggcgagca tcggctacct gggtatgtcg cttgccgtcc gagccaacac ccgcatggcc
     7021 gcggctgccc gggacgccgg tcgcgaccca gcgatgaaga tcgccttccg caccggaggc
     7081 gtggtcggga tggccacggt cgggctcggc ctgctcggtg cgtctgtcgt cgtgttgatc
     7141 tacaaggagg acgcgccgtc ggtgctcgag ggcttcgggt tcggcgccgc gttgcttgcc
     7201 atgttcatgc gggtcggagg cggcatcttc accaaagccg ccgacgtcgg cgccgacctg
     7261 gtgggcaagg tcgagcagaa catccccgag gacgacccgc gcaacgccgc cacgatcgcc
     7321 gacaacgtcg gcgacaacgt cggcgactgc gccggcatgg ccgccgacct gttcgagtcg
     7381 tacgccgtca cgctcgtcgc ggcgctgatc ctcggcaagg cggccttcgg ctccgagggt
     7441 ctcgtcttcc cgttgatcgt cccggcgatc ggtgcgctga ccgcgatcct cggtatctac
     7501 ctgtgcaagc cccgcgccgg cgagaacggc ctcacgacga tcaaccgcgc cttctacatc
     7561 tcggcggcag tgtcggcggt gctgtgcgtg ctggccgcgt tcatctacct gccgacctcg
     7621 ttcgacgacc tgcacggtgg tggcggggtc tccctcgtcg agcagttcct gcagacctct
     7681 cccggcgtgg acggcaaccc ggcgctgatc gcttcgatcg ccgtgatcat cgggatcgtg
     7741 ctggcatcgc tgatcctgac cctcactggc tacttcaccg gaacggagca ccggccggtc
     7801 cgggacgtcg gcaagacctc gctcaccggc ccggcgaccg tgatcctttc cggcgtctcc
     7861 gtcggcttcg agtccacggt ctacaccgcc ttggtcatcg gtgccgcagt gttcggggcc
     7921 tacctgctgg ggtcgggggc gattctcgtc tcgctgttcg cggtggccct cgccggctgt
     7981 ggcctgctca cgaccgtcgg cgtcatcgtc gccatggaca ccttcggccc ggtgtcggac
     8041 aacgcccagg gcatcgccga gatgtccggg gacgtgcacg gtgagggcgc ggccatcctg
     8101 accgagctcg acgccgtcgg caacaccacc aaggcgatca ccaagggaat cgcgatcgcg
     8161 accgcggtgc tggccgcgac cgcgttgttc ggctcgttca ccgacaccgt cggttccgaa
     8221 gcggagaagc tcaccggcgc cggcgatctg tccgccggcg acgtgctcga cttgctggga
     8281 gtcctcaacg tggccgaccc gaagaacctc gtcgggctcc tgatcggtgc gtcagtcgtg
     8341 ttcctgttcg ccggtctggc gatcaacgcc gtcggccggg cggccggcgc ggtggtcttc
     8401 gaggtacgcc gccagttccg cgagatcccc ggcatcatgg agggaaccgg ccgtccggag
     8461 tacggccgcg tcgtcgacat ctgcacccgc gactcgctac gcgaactcgc aacgcccgga
     8521 ctgctggccg tgctggcgcc gatcgccgtc ggattcggcc tcggcgtggg tccgctgggc
     8581 tgctacctgg ccggtgcgat cgggaccggc acgttgatgg ccgtgttcct ggccaactcc
     8641 ggcggggcgt gggacaactc gaagaagctc gtggaggacg gctaccacgg cggcaagggc
     8701 tctcccgcac acgaggccac catcatcggc gacacggtcg gtgacccgtt caaggacacc
     8761 gccggcccgg cgatcaaccc gctgatcaag gtgatgaacc tggtgtcgct gctgatcgcg
     8821 gccgcggtgg tcaagatgac gtacggcgac gacagcaaca cggcgctgcg gatcgtcatc
     8881 gcggtcgtgg cggcattggc aatcgcgacc gcggtgtaca tctcgaaacg ccgcccggtc
     8941 gcgatcggcg acgcggacac cgagcccgct gagtccacca gcgtctgacc gacaacctcc
     9001 ggactgggca gcgggcttgc gtggcccgtt tactaggatg cggcttctag ccggctttgg
     9061 aggaggatcg cctgtctcgc acggtgttcc tgcacgtcgg cgtcgccaag acggggacga
     9121 cgtacctgca gcggctgttg ttcgccaacc gggaactgct ccggtccgca ggtgtgctct
     9181 acccgggcca gcggccggag gcgcacttct tggcctcgat ggatctccgc gacgcgggcc
     9241 tcggcggtca caagtatccg gccgcggcgg gcaggtgggc caagctcgca aacaccgtca
     9301 actgcttcga cggcaccgcg gtgatctcgc acgagaccct gtcgcgggcg cgacggccgg
     9361 cgattcaacg ggccatcgaa gccttcgaga ccgatgacat ccgagtggtc ctcacgacgc
     9421 gtgacctggg tcggcagatc cctgcggtgt ggcaggagaa cgtcaagaac cgcaacccgc
     9481 agacgtacgc cgacttcctc catggcgtgc tcgtcggggt gcctccggcc aagccaggac
     9541 cgggcaagcc ggatctggtt cggttggtgt cgttctggtg gccgcagaac ctcccgaaga
     9601 tggtcgggcg atgggcgccg gtggtgggca ccgacaaggt cgtgctggtg acggttccgc
     9661 cgctcggctc tccacgggac gagctgtgga agaggttcgc gaccgctgtc gagcttccag
     9721 tactcgacta cgagttcgac gtcgtcggcg agaacgtctc actgggggct gccgaggcgg
     9781 agctgatgcg ccgcgtcatc gcgtacgtcc cggcagaggt cgactggccg aggtggaaga
     9841 accgagtcaa gcgagggttg ggcgaacgga cactggcatc gatgggggct ggtggacgga
     9901 tcctcgtgcc gaagcaatgg cacgatgtcc tcgcggacat ctccgagcag atgatcgccg
     9961 acctggcggc cagcggtgtg cgggtcgtcg gtgacctgga cgatctccgg ccacggttcg
    10021 accaggaaga cggcctgctg ccggatgacg tcagcgacgc cgagctcctc gagtgcgcgt
    10081 cggccgcgct cgcccagatg gcgagtgcac cgcccgaaca cgtccggagc gttcacaacg
    10141 ccccgacaca aagaccggag aacggtgacc cgcaacgcac tcgcggtgag gcgaacgtgc
    10201 agaaggagaa gcctcgacgt ggctgagcgt gtctacatcc acatcggggc cccgaagacc
    10261 ggcacgacgt ccctccaaca ggcgctttat gccaaccgtg agcggatgga ggaggtcggt
    10321 gtcctctacg ctccggaacg acacgatgcc caccactccg cggtgttgga cctgcgcgac
    10381 gcgtggcaac gcgagaacgc gtcccacctg cgcggcgcgt ggaagcaggt cgtccgacag
    10441 gtccggcaat gggacggccc cacagcggtg ttgtcgagtg agttgttcgt gtacggcagc
    10501 aagcaggagg ccgcccgggc gttatcggca ttcggcgacg ccgaagtcca cgtcatctac
    10561 accgcccgtg atctcgtccg ccaggtaccc gctgtctggc aggagcggat gaagaatcag
    10621 tacacgatgc cgtacgggcg cttcgtccag gacgtcatcg gtccggccag gtcgccgatg
    10681 gcacgtggtt tctggagtgc acaggatgcg cccgaggcgc tgaagcgctg gtccaagggc
    10741 cttccgtcga gccacgtcca cgtcgtcacc gcgccaccgc cggggtcatc gccggccctc
    10801 ttgtgggacc ggttcctgtc cgtgttgggt ctcgagggcg ccgcgtaccc ggccgacgtc
    10861 tcggtcctga acccgtcctt gggcgccgtg gaggcggagg tgctgcgccg tctcaaccgc
    10921 cgccacgggc aggacctcgg cccgggcgcg taccggatga tcgtccgcaa cggacttttc
    10981 gacgtcctcg accacgtcgt gcccgacaag atcaagatca ccttgcgaag cgacgagcac
    11041 aatgccctgg tcgccaaagg gaggcagatc gcggacggga tacgtcgcgg cgggtatgac
    11101 gtcgtgggtg acctcgccga cctggtgccg ccgtctcgca cgccggaaga cgacgccgac
    11161 gcggcgcgaa gacctgacga cgtcagggca gccgagatgg tgaacgtgct cgtcgacgtg
    11221 gtgtacgcgc tgctcgagga gcgccgggca ctcaaggaca gccacgttcc gcgggccagg
    11281 agacggcggc ggcggtccct cgagcttcgt gcgagcgggg acgaacggtc ctgagcggtc
    11341 cggctgggtc agcggtgcag cagcccgagc cgccgtagca gcttcccgac ccgggaccgt
    11401 agacgccggt acccgccggc gaaccggtca cgcagcggcc gcgatcgcgc accagtgtct
    11461 gtccccgcct tctgcagctc gacgatcgtc cggcgttgcc gtcccgcgtg ctgcagcagg
    11521 ccggttgccg cccggatcgc tgcggggccg aggtcggcgt ccgtcagctc ggtcggcgag
    11581 acgtacgtcg cgtggtctgc ggggttcggc atcaggtctt cgaggtcgcc gacaaccggg
    11641 tagccggctt cggtgagctc gtcgatcagc cggcgcgacc gcttcatcac ccagtcgagc
    11701 cgacccggcg gcagcgtcag ccggggtggc tggccgccga ggatgtgttc ggccagcaac
    11761 agcctgacca aggggtcgta gttctccgcc ggcatcccct gcaccgatcc gttcatgcgg
    11821 cgtacgagct cggtctcggc cacgcccatc gacgggttcg agctcgtcgt caccgagtcg
    11881 tacgcgtccg gttgcacgcc gatcacctcg cagaagcgat gccacaacgt ggccctgggt
    11941 gcacccggct gcggaagcgt gaggacgtgg atgttctgct tcggcacgaa caccgcccag
    12001 cggcgtagca cgtgggccgc gtcgtgcatg ccccagaaca gctcgccgaa aggcttgggc
    12061 gcatccagcc ccaacgtgat caggtcgtcg acgaacttct cgagcgtcac ctcgtgcttg
    12121 tgcttgacgt gctcttgcca gtcggacacc agctgccggg cgaggtcccg ggccgagaag
    12181 atgacatgca cgtcggccgg ggcgacgctc gccacgcctg ccgagatccg ctcgaccgcg
    12241 gctccgccca gcagctcgtt cgagatgacg atcgtctcac cacgccaggc aacggttcgg
    12301 tcggcgacct tcttccactc gcctttgaac gcgcgcgccc gttggccacc ccatccgaga
    12361 cccttgaagt cctgcatgga ccggaacgac tgggcgtggt cgtcgtacgg gtagaggacg
    12421 ccatgcttcg cgagggcttc cttgttctgc agcaggacgt gctgcaggta cgtcgtcccg
    12481 gtcttcgggg caccgatgtg gaagtacacc cgctttccag gcggtccggc ggggtctggc
    12541 gtgggcacgc gctcatcgta gaggcgctgc ccggattggc gaccgaagcg gcccggacgg
    12601 taccgtgttg gcgccgacac gcccccttcc gaggactgaa ccttgactga ctccgccgcc
    12661 gagaccgccc acgttgcttc gggctggcgc gtcgtgcagc gcacgatcat ccgcccagac
    12721 caggagctcg acatccgccc gttgtacgtc acggggcttt ccggtttcgg tggcgcggcg
    12781 agcaccgcga gacagtccgg cgaggagatt cccgacgtcg agcggcagat ccggggcaaa
    12841 ggcatccctg ccaacctgta cgacgactcg gccgacgagg gcgtcgaggc gctgggggcg
    12901 ttcgggacga tcaccgaggc cggagcggtc gcgaacgcgg agcac
//
LOCUS       DATBFZ010000218         4943 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_3094809, whole genome shotgun sequence.
ACCESSION   DATBFZ010000218 DATBFZ010000000
VERSION     DATBFZ010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 4943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4943
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3094809"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..503
                     /locus_tag="VH419_06600"
     CDS             <1..503
                     /locus_tag="VH419_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020392903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HEY7043320.1"
                     /translation="TVNFGDDVFMNPWPFVAELYQLTQERGVVPEFELFDLGHVHALH
                     RLLGTYGPPFGGRVHCDLVMGVPGGMPGTTDALVAAVQALPTEVTSWSATGIGRTTLA
                     VALAALSRGGHLRVGMEDTLTFARGVPVSHNDELVARAASLATIAQRPPMSTDEARDF
                     LQIKPR"
     gene            721..1215
                     /locus_tag="VH419_06605"
     CDS             721..1215
                     /locus_tag="VH419_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012918653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FABP family protein"
                     /protein_id="HEY7043321.1"
                     /translation="MPFEIPADLHPDLMPIAFLLGQWHGNGHGDYPTIDPFQFRQEAG
                     FTHDGRPFLHYLSRTWLLDEDGNDVRPAALETGFFRPQPAGALEVVLTHPTGYSEIYY
                     GKVEGAKIELTTDAVARTQSAKEYRGGHRLYGLVEGDLLWTFDMAAVGQDIQPHIWAR
                     LTRS"
     gene            1324..1758
                     /locus_tag="VH419_06610"
     CDS             1324..1758
                     /locus_tag="VH419_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017936590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEY7043322.1"
                     /translation="MGLQETLRAKGYRITPQRQLILDAVTDLGHATPEEAFAWVTQRS
                     SGVNISTVYRTLELLEELGLVKHAHLSHGAPTYHAATAPEHVHLICRTCGTITEVSPQ
                     TVEPLVDQLQRDQGFVADIGHLTVFGTCRGCSAANDVKEPSA"
     gene            1755..2693
                     /locus_tag="VH419_06615"
     CDS             1755..2693
                     /locus_tag="VH419_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020579201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HEY7043323.1"
                     /translation="MTGRTHRSPLLDLPRAVEADGPDAGVAAHYGDPFREQKALAAGD
                     GFVDLSHRGVVSVTGPDRLSWLHSLTSQHLEQLPPHVWTQALVLSPQGHVEHEFAGYD
                     DGETFWAHVEPGRAEPLVAWLDSMRFMLRVEVADVSTDWAVLARGETSVELVERGRLA
                     DVAVGAPCGTWAYDALRIARGEPRFGVDTDHRTIPNEVGWIGTAVHLDKGCYRGQETV
                     ARVHNLGRPPRRLVLLHLDGSVDALPQQGASVTHDGQQVGVVGSTARHHELGPIGLAL
                     VKRSLPVEATLLADGIAAAQEVVVDPDVGLHVRARL"
     gene            complement(2735..4000)
                     /locus_tag="VH419_06620"
     CDS             complement(2735..4000)
                     /locus_tag="VH419_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY7043324.1"
                     /translation="MASPAMDLLRFATAGSVDDGKSTLIGRLLLDSKAIFEDQLEAVE
                     HTSKSRGYDYTDLALLTDGLRSEREQGITIDVAYRYFATPNRKFIIADTPGHVQYTRN
                     MVTGASTADLGLVLVDARQGLTEQSRRHAVILSLLRVPHLVLCVNKMDLVDYSQDRFH
                     EIHREFTSFATKLSIPDLTIIPISALKGDNVVERSTAMSWYAGPSLLHHLENVHVASD
                     RDLVDARFPVQYVVRPKSDDYHDYRGYAGRVAGGVIKAGDEVLVLPSGLTSTVTGIDL
                     FDKELDEAFPPMSVTIRLDDDIDVSRGDMICRPQNAPSPAQDIDAMICWMSTSPLKPR
                     QKLAIKHTTRMARTMIKDVQYRLDVNSLHRDLETKQLGLNEIGRVQLRTTVPLLCDPY
                     DKNRTTGSFILIDEGTGVTVGAGMINSAS"
     gene            complement(4000..4911)
                     /gene="cysD"
                     /locus_tag="VH419_06625"
     CDS             complement(4000..4911)
                     /gene="cysD"
                     /locus_tag="VH419_06625"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HEY7043325.1"
                     /translation="MQTHADYRLSQLDQLEAESIHIFREVAAEFEKPVLMFSGGKDSI
                     VMLRLAEKAFYPAKIPFPVLQVDTGYDFPEVLQTRDNWVSRLGVRLVVASVEQAIKDG
                     IVVDDGKTSRNRLQIGTLLHTIEEEGFTAAFGGGRRDEEKARAKERVYSHRDDFGQWD
                     PKNQRPELWSLYNGRIHAGEHMRIFPLSNWTELDIWHYIGREGIEIPSIYFAHERRVF
                     RRDGMLFSESEHNPLRAGEEVEERTVRFRTVGDLTLTGCVESTADTIDLIIDEIAVAR
                     VTERGATRGDDRFSEAAMEDRKREGYF"
BASE COUNT          751 a         1664 c         1697 g          831 t
ORIGIN      
        1 gcacggtcaa cttcggcgac gacgtgttca tgaacccctg gccgttcgtg gccgagctgt
       61 accagctgac gcaggagcgc ggcgtggtcc ccgagttcga gctgttcgac ctcgggcatg
      121 tccatgccct gcacaggctg ctggggacct acggcccgcc attcggtggc cgggtgcact
      181 gcgacctggt gatgggcgta cctggcggca tgccggggac caccgacgcc ctggtcgccg
      241 cggtgcaggc gctgccgacc gaggtgacgt cgtggtcggc caccggtatc ggccgtacga
      301 cgctcgcggt cgcactggcg gcgctgtctc gaggtggcca cctgcgggtc ggcatggagg
      361 acacgttgac gttcgcacgc ggtgtgcccg tgtcgcacaa cgacgagctg gtcgcgcgcg
      421 ccgcctcgct ggccaccatc gcccagcgcc cacccatgtc caccgacgag gcccgcgact
      481 tcctccagat caaaccccgc tgaccctccc gccgagccct gccgaggtgg cagctttcgt
      541 tgaatttcta cggcgtgtcg caacgggatt gccacctcga tcggcaggga ggcggcgttg
      601 tcgcggatag actcgccagc gtggaggcct tctacgtcag tttgttggtg ctcggcgcag
      661 ttctggtgat gagctcggcg gcgtacgtcg tctacagact cgtccacggc caggcctgac
      721 atgccattcg agatccccgc cgatctccat cccgacctga tgccgatcgc tttcctgctc
      781 ggccagtggc acgggaacgg ccatggcgac tatcccacga tcgacccgtt ccagttccgt
      841 caggaagcgg ggttcacgca cgacggccgc ccgttcctgc actacctcag ccgtacctgg
      901 ctgctcgacg aagacggcaa cgacgtccgg ccggcggcgt tggagaccgg cttcttccgg
      961 ccgcagccag caggtgccct cgaggttgtc ctgacccatc ccacggggta ctccgagatc
     1021 tactacggca aggtcgaggg cgccaagatc gagctcacga cggacgcggt cgcgcggaca
     1081 cagtccgcga aggagtaccg cggtggccat cgcctctacg gactcgtcga gggcgacctg
     1141 ctctggacgt tcgacatggc ggcggtcggc caggacatcc aaccgcacat ctgggcccgc
     1201 ctcacccgga gctgatgagg ccccggtcat gggccgaggt ggcgatcccg gttcgacacg
     1261 ccgacaaact ccaacgaaac ctgccacctc ggcgggggtg aggaaggacc ttaaactggc
     1321 ggggtgggac tgcaggagac gttgcgtgcg aaggggtacc gcatcacgcc gcaacgccag
     1381 ctcatcctcg acgcggtcac cgaccttgga catgcgactc cggaggaggc gttcgcgtgg
     1441 gtcacgcagc gctcgtccgg tgtcaacatc tccaccgtct accggacgct cgagctgctc
     1501 gaggagctcg gcctcgtcaa gcacgcccac ctcagccacg gggcgccgac gtaccacgcc
     1561 gctaccgccc cggagcacgt ccacctgatc tgccgaacat gcggcacgat caccgaggtc
     1621 tcgccccaga cggttgaacc cctcgtcgac cagttgcagc gcgatcaggg cttcgtcgcc
     1681 gacatcggac acctgaccgt cttcggcacc tgcaggggct gctcggcggc gaacgacgtg
     1741 aaggagccca gcgcgtgacc ggacgcaccc accgcagccc gttgctcgac ctcccccgcg
     1801 cggtcgaggc cgacggcccg gacgccgggg tggcggcgca ctacggcgac ccgttccgcg
     1861 agcagaaagc gctcgccgcc ggcgacggtt tcgtcgacct gtcgcatcgc ggtgtcgtgt
     1921 ccgtcacggg tccggaccga ctgagctggc tgcactcact caccagccag cacctcgagc
     1981 agctgccgcc gcacgtctgg acgcaggcgc tggtgctgtc tccgcagggc cacgtcgagc
     2041 acgagttcgc cgggtacgac gacggcgaga cgttctgggc ccacgtcgag ccgggccggg
     2101 ccgagccgtt ggtcgcctgg ctggactcga tgcgcttcat gctccgggtc gaagtcgccg
     2161 acgtctcgac cgactgggcc gtgctggcgc gcggggagac gagcgtcgag ctggtcgagc
     2221 gtggccgact cgccgacgtc gcagtcggcg cgccctgcgg gacgtgggcg tacgacgctc
     2281 tgcgcatcgc gcgcggcgag ccgcggttcg gagtggacac cgaccaccgc acgattccca
     2341 acgaggtcgg ctggatcggc accgccgtcc acctggacaa gggctgctat cgcggccagg
     2401 agaccgtcgc cagggtgcac aacctcggac gcccgccgcg acggctggtg ctgctgcacc
     2461 tcgacggctc ggtcgatgcc ctgccgcagc agggcgcgtc ggtgacccac gacggccagc
     2521 aggtgggcgt cgtcgggtcg accgcccgcc accacgagct gggtcccatc gggctggcac
     2581 tcgtcaagcg cagcctgccg gtcgaggcaa cgttgctcgc cgacggcatc gcagccgctc
     2641 aggaggtcgt cgtcgatcct gacgtgggac tgcacgtccg tgcgcggctc taggtggtgc
     2701 cgtccgccgt ccgcaccggt cgccccgtgg aacgctagct cgcggagttg atcatccccg
     2761 cgccgacggt gacgcctgtc ccctcgtcga tgaggatgaa cgagcccgtc gtgcggttct
     2821 tgtcgtaggg gtcgcacagc agcggcacgg tcgtccgcag ctggacacga ccgatctcgt
     2881 tgaggccgag ctgcttggtc tcgaggtcgc ggtgcaggga gttgacgtcc agccggtact
     2941 gcacgtcctt gatcatcgtg cgcgccattc gcgtggtgtg cttgatggcg agcttctgtc
     3001 gcggcttcaa cggcgacgtc gacatccagc agatcatcgc gtcgatgtcc tgtgcggggc
     3061 tcggggcgtt ctgcgggcgg cagatcatgt cgccccggga gacgtcgatg tcgtcgtcga
     3121 gacggatcgt caccgacatc ggcgggaagg cctcgtcgag ctccttgtcg aaaaggtcga
     3181 tgcccgtcac tgtcgaggtc agcccggacg gcagcacgag cacctcgtca cctgctttga
     3241 tgaccccgcc ggccacgcgt ccggcgtacc cgcggtagtc gtggtagtcg tcggacttgg
     3301 gtcgtacgac gtactgcacg gggaaccggg cgtcgaccag gtcgcggtcc gaggcgacgt
     3361 ggacgttctc gagatggtgc aggagggacg ggccggcgta ccacgacatc gccgttgaac
     3421 gctcgacgac gttgtcaccc ttgagggccg agatcgggat gatcgtcagg tccgggatgc
     3481 tcagcttcgt ggcgaacgac gtgaactcgc ggtggatctc gtggaaccgg tcctgcgagt
     3541 aatcgacgag gtccatcttg ttgacgcaca gcacgaggtg cggcacccgg agcagcgaca
     3601 agatgacagc gtgccgtcgc gactgttcgg tgagcccctg ccgggcgtca acgagcacca
     3661 gcccgaggtc cgcggtcgat gcacccgtga ccatgttgcg ggtgtactgc acgtggccgg
     3721 gggtgtcggc gatgatgaac ttgcggttcg gggtggcgaa gtagcggtac gcgacgtcga
     3781 tcgtgatgcc ctgctcgcgc tcggaccgca gcccgtcggt gagcagggcc aggtcggtgt
     3841 agtcgtatcc gcgcgacttc gaggtgtgct cgaccgcctc gagctggtcc tcgaagatcg
     3901 ccttcgagtc cagcagcagg cgcccgatca gcgtggactt gccgtcgtcg accgagccgg
     3961 cggtggcgaa ccgcagcagg tccatggcag gtgaagccat cagaagtatc cctctcgctt
     4021 gcggtcctcc atggcggcct cgctgaagcg gtcgtcgcca cgggtggcgc cgcgctcggt
     4081 gacccgtgct accgcaatct cgtcgatgat caggtcgatg gtgtcggccg tcgactcgac
     4141 gcagccggtg agcgtgaggt ctccgaccgt gcggaaccgc accgtccgct cctcgacctc
     4201 ctcgccggcc cgcagcgggt tgtgctcgct ctcgctgaac agcatgccgt cgcgtcggaa
     4261 gacccgccgc tcgtgggcga agtagatgct cgggatctcg atgccttcgc ggccgatgta
     4321 gtgccagatg tccagctcgg tccagttgga cagcgggaag atacgcatgt gctccccggc
     4381 gtgaatgcgc ccgttgtaga gcgaccagag ctccggacgc tggttcttcg ggtcccactg
     4441 gccgaagtcg tcgcggtggg agtagacgcg ctccttggcc cgcgccttct cctcgtcccg
     4501 gcggccaccg ccgaacgctg cggtgaatcc ttcctcctcg atggtgtgca gcagggtgcc
     4561 gatctggagc cggttgcggg aggtcttccc gtcgtcgacg acgatgccgt ccttgatggc
     4621 ctgctcgacc gacgcgacca cgaggcgcac accgagccgg ctgacccagt tgtcccgggt
     4681 ctggagcacc tcggggaagt cgtagccggt gtccacctgg agcacgggga acgggatctt
     4741 cgcggggtag aacgccttct cggcgagccg gagcatcacg atggagtcct tgccgcccga
     4801 gaacatcaac acgggcttct cgaactcggc cgcgacctcg cggaagatgt ggatggactc
     4861 ggcctccagc tggtcgagct ggctgagccg gtagtcggcg tgggtctgca tgcggtgggt
     4921 gaacctctcg tcagggcagc agg
//
LOCUS       DATBFZ010000219        17298 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_2765751, whole genome shotgun sequence.
ACCESSION   DATBFZ010000219 DATBFZ010000000
VERSION     DATBFZ010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 17298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17298
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2765751"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            647..1345
                     /locus_tag="VH419_06630"
     CDS             647..1345
                     /locus_tag="VH419_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043326.1"
                     /translation="MNAKWWRQGGLLGIAFVFVWLVAVLVRGDPPAYDHPVGELRAYW
                     HDHGDRYLVAQYVVVLAALTMLIPYMVAFTTLLGRAEGEPRIWSRISLIGGSLFVAVA
                     LAAQSAWAALAIDVDRLSDDAVVTLMQLDQGAYQAPPPLYGLFVAAASIVIVRTRAMS
                     RWLGFMGLAIALGMLLYPLSLLFENPDNVFTLLYFFAYLAGQAWLLTGAIFMLRMREE
                     EAVLRPSRLASPEP"
     gene            1471..2664
                     /locus_tag="VH419_06635"
     CDS             1471..2664
                     /locus_tag="VH419_06635"
                     /inference="COORDINATES: protein motif:HMM:NF025114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEY7043327.1"
                     /translation="MDVVVVGAGQAGLAVSHALTIRGVDHVVLEADRVASAWRHRWDS
                     FTLVTPNWTLALPDAPYSGEDPEGHVSRDQIVDYLEWYAAAHAGEIRVGVRVDQIAPG
                     TGGRFVLDTSDGVMSSDQVVVCTGAFRVPHMPPHAGAFPPHVTVLDATSYRAPGELSA
                     AGVLVVGSGQTGVQLADELRASGRNVTLACGRAPWAPRRADGVDIITWLARTSYFEDR
                     VTDLPHPWSRLAANAQLTGAGGGHDLHFRVLQQQGVTLAGRLTGVDAGRALFADDLAD
                     SVAFGDARYAHIRALVAAELGPDAPFLPDPEPFVATAPTELDLADVGTVIITSGFRPD
                     HARWIQFPVFDELGFPIVGDDLATAVPGLFFCGVHFLRNRRSSLLWGVGDDAAVVADT
                     LARQQ"
     gene            2828..3880
                     /locus_tag="VH419_06640"
     CDS             2828..3880
                     /locus_tag="VH419_06640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="squalene cyclase"
                     /protein_id="HEY7043328.1"
                     /translation="MQWLLQGDPAIRWQVLRDLVEASETEVATERARVEHEGWGARLL
                     ALRAPDGLWANGACFPDTAAFAAATARALAAGEPPPPFPSPDAEPAEAPTDLSRAGGQ
                     PWTATYRVLLDLCDLGIPPASPVMQETAELVARNCRWECDGLPFFAGEEDCCINAGTI
                     LIGVYLGVDVDPVVRRLVADQMPDGGWNCWAETRPAPGSFASTLDVIDALLRWERQTG
                     GSEEVRRARRNGEEYLLRRNLFRSLRTGELVNPQWTLFSYPPRWHYDLLKAAEYFARR
                     GGTPDPRLAEAIERVRAKRRPDGRWPLENTHPGAVHFRFEEPDGTPSRWNTLRALRVL
                     RWYDAPAHSDRASQAT"
     gene            complement(4011..4706)
                     /locus_tag="VH419_06645"
     CDS             complement(4011..4706)
                     /locus_tag="VH419_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020574363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY7043329.1"
                     /translation="MALRLEAVTFDVADASAVAAFWARLLRREVHAESGSALVPGDQT
                     QVGLRFVTSDIQQIGPRRLHLHLTSSSLEDQQRIVEMALGLGGRHIDVGQEPHAEFVV
                     LADPGGNELCVIEPDNTFLAGTGRLGEVTCDGTRDVGLFWRDALGWPLVWDQDEETSI
                     QSRLGGTKVSWGGPPVEPKRGRNRQRLDLLTADPHTEAGRLLSLGATRVADLDDGLEL
                     ADPDGNEFGLHAG"
     gene            complement(4904..5785)
                     /locus_tag="VH419_06650"
     CDS             complement(4904..5785)
                     /locus_tag="VH419_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020390967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HEY7043330.1"
                     /translation="MAEDPFVTHRGLLFTVAYEMLGSATDAEDVVQDTWLRWDAIGGD
                     ARDDVRDPRAFLVRMVTRKALDRLRSNARRRELYVGEWLPEPLLTAPDVAEDVELAES
                     VSIAMLTVLETLTPTERAVFVLREVFDTPYDEIAQAVGKSAGAVRQIGHRAREHVAAR
                     RPRMEVSRSQRQEVVDRFLAAVTQGDLQGLLDVLAPDVVLVADGGGVVQAVVNPVVGA
                     KKVANLLRPFPRLEPGARILPTLLNGSVGARIMGTSDGHDTAISFVVEQGRISRIYAV
                     RNPAKLEAIDVEATITR"
     gene            complement(5794..6378)
                     /locus_tag="VH419_06655"
     CDS             complement(5794..6378)
                     /locus_tag="VH419_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017936698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEY7043331.1"
                     /translation="MTGATRIPAAEITGFKGMLMKRLAKRSLGQVPTSLGVYWHNQRV
                     LMAMAGAGGKVQKWDACDEQLKSFAHMAVAAQVGCSWCLDFNYFEAFNKNLDMAKARE
                     IPRWRESDMFSPLEREVLTYAEAMTVTEPTVTDEQLASLRQQLGDAAVVELTAVIAFA
                     NLTTRSNVALGIESDGFAAACGLKPLAQPAAVAS"
     gene            6800..7993
                     /locus_tag="VH419_06660"
     CDS             6800..7993
                     /locus_tag="VH419_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043332.1"
                     /translation="MRCVGGCGCQDRHLDPTGGAGELVFGWAGVLVVDVDYEEFADLV
                     SGRPVFHEYGGPTYRWTRGILRYMQGLGSPFLQSHGLRLLIAAQDLIEAPTGSHEAFT
                     AEARRLAEPDAGGLSCEDLLAAAAEVEVLRLLPRDPTLELMSPGSFHRLVLKQVHPDP
                     DAPARRGFEALARRIGDDDACELFPILTFLAFILNDPCETFDSLVRMAELSAQPFKQL
                     TAAQLIDWLGWADDYERCWDLVAAGEPVGTPYLVDPLRAAMRLHGRSELLEVLARPSS
                     HLNALPEAQARAVQPPIIVFPRRDGGVHHHRNGVALDDVDWAQEALVNLGRYGAAERL
                     TDNHSETNPSYCTHLGCPHHAVGLCNRWYLAPTTAQGHDDCSFPPVFAASAGMYPAEI
                     VGRLS"
     gene            7990..8850
                     /locus_tag="VH419_06665"
     CDS             7990..8850
                     /locus_tag="VH419_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043333.1"
                     /translation="MTSGSLAVVVIEDEPIEGVADRVARMPSDAEDDIATGDLVDGLA
                     ADRISLPTERGLPDLVRRIVGRLRGLQLVDDLGRVDSSFELLGLHVPPGGRAALEMRR
                     SATVDRQISVTAMGLGFGGGRRFTIAMDDDIGERSGCMRVLQRVELHVRRFANEADGA
                     EPIIRTDVVGWGARELTLLADCPFCGGVGEPGPLEYEQDLPQALDLRTFDGPVKRETS
                     YSLDGSRKTDIGLSLAVPGAAETVHVGFQLEQQTRLECTATYSFPPGRFYVPYRSRDA
                     PATLPYWAVR"
     gene            8852..11614
                     /locus_tag="VH419_06670"
     CDS             8852..11614
                     /locus_tag="VH419_06670"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HEY7043334.1"
                     /translation="MGALARTIAVLPAGWAEHQSDRIAELVSSDDALEARAWRLWLEA
                     EWAKHGGEFGLAEQALGAADQLANGIRTRQLWDLESHGLRGRLLHDCGEPGRAHEHIN
                     WAMQNWLRVRTVLQSPNPDTGDFALDLLHSAHAMVSAIVDPHEIAELAKLLDAAPFEV
                     TAGMWASDRVPSQTVEYVARAIELEAKVDSYSASRRLANDVLAWMRSWELESSEALRF
                     QAGVRLGLSSAALGTHEFATAVAQADDGLALAGQLPEGTDRSGVQAELKANRGNALAM
                     LGRTRDAAADYDTAMVAFDEAGDQTAALSMRLAKLTVRHDAGEDVTDVEVQAMIAELE
                     DEVHRVPNSGRLRGELERTRRWWLSLLAQKESDDTERAVGLIEVLRDDQPVLRTGTRS
                     QDHVLDLLCRAATVLRARLRALADTVVVVLEPGIRTGDRLYPPVFLVMAGGESTLVPS
                     GDAAGRLAELGRIASIERERLLTGETTLNSPASGRLTEAAVAAWKAIPSVVTERVLAA
                     ETVLYMPSGVNAIDALPFELLRHEAGWLGMTHVVARAPSFQLLEQMLTPNARAHTNDS
                     RAVIATVRQDSQLGVLSEALREADIVERAAEYMGLTADRRETLDTDALLDVLRRNALV
                     HYIGHGFASEIGEVLPLTSDTGVYVDQLSDHDGMPSPLVFFDACMLGRVRHLEGGRQR
                     GWTLALLERGAPAVIGALAPVPDSACSAVAKAFYGAVLDAPVGVAMRNARAGLEQAGL
                     HPLIAAAYVVHGDPSARLTPGHRGGTASRVCRWPSLAARFLATEHPDDRAEIVSASCV
                     PAAVASWANGVDVSPAEFASAVEMTIAEDPEGAAICRILLALKRFDQNPNDDDELDVA
                     WLVAEAVGDRYAQLHLIDRYAPALIRRHPDLRAHLPSETRFSLNALGGGRGRLAGLAE
                     RLAHVC"
     gene            11922..12350
                     /locus_tag="VH419_06675"
     CDS             11922..12350
                     /locus_tag="VH419_06675"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEY7043335.1"
                     /translation="MSAYFGLDLDRLLGLWSVPPADRHDAYADFAALYEDPVTINDSP
                     TPLQDLVARAQAINEAFSDQQSEILDVVAARSKLAVAFRRHATHTGTWSSPLGDLPAT
                     HERVTIMGLDIFTITDGRITAIRVLGDDLAVLLTAAGRRL"
     gene            complement(12540..12613)
                     /locus_tag="VH419_06680"
     tRNA            complement(12540..12613)
                     /locus_tag="VH419_06680"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12577..12579),aa:Phe,seq:gaa)
     gene            complement(12718..14151)
                     /locus_tag="VH419_06685"
     CDS             complement(12718..14151)
                     /locus_tag="VH419_06685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043336.1"
                     /translation="MSDRLAEDPEDVVFNGLNGSTGEYLWPPTTRRSLAAGLRGGATH
                     HADELDELRYRDAEPAFGVVFGVDDLDDLASTGWALVAAQDTPPDVLETLAPLRELRA
                     EQAGERYREFIGPDGLAPGEDKRAFLARNGIGPGMPVNPVRLPYYLLLIGGPEQIGFT
                     FQYQLDVEYAVGRLSFDHERLERYVSAVVAAEATDRPSTRRSRFFGPAAEDDRATILS
                     ATELVAPLGAALQHDVREWDIDVLPPGACTKDRLLQLVSDDDAPALLFTASHGLGFDA
                     DDERQRDEQGALVCQDWSGPADNGQEPIPPDCFVAGSDVEALDAVNTELVMTFACFGA
                     GTPHRDEFSRPGDGAAAQELTQVPFVSKLAEQLLTHAHSSTLAFVGHVERAWGYSFLA
                     PGVGSQTDVFASCLQALMAGWRVGHAMEFFDDRYSSLSTALHDMLHEARTEGLRLDDV
                     ETARMWTAVNDARSYVVIGDPAVRLRT"
     gene            complement(14148..15650)
                     /locus_tag="VH419_06690"
     CDS             complement(14148..15650)
                     /locus_tag="VH419_06690"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY7043337.1"
                     /translation="MLKLQENPFVGPRPFRDTDIFYGRERETKNLANLLISGRVVLLH
                     SPSGAGKTSLVQAGLVPFLKEDFQICSAKKQYFSALRVHEPPPDSASSDRRFVRSVIN
                     GLLQGHETRVSALDSMTVSDALALRRERSDDPRDQLIIIDQMEEVLTLDPANRDAQTL
                     FFRQLGEALDDPRRWALLAMREDYMGGLDRFLPLLPGRLRTTYRLDFLSVSAAERAMV
                     EPAKARNVSFLPEATDALLDDLRKIKVEDPDDSVRVDRAPYVEPVMLQVVCHRLWRRL
                     DSDDSRQSAEITAADVERFGSVNRALRRYYADAVVEVAGADIERQRAIRDWFEDELIT
                     EAEFRSQTRTGPELDDRSDVLAALQDRYLIRGDQRAEAWWELSHDRLIKPVLSDNASW
                     RRTTLSPWRVNAHKWHRRGHDRNYLLERSWLEPAQRQLAALGDRGTSYEKAFVEESAE
                     RVRTSDELSQQQAALAKMRAGLSKSLQRTTLFLTLSICLNIILVLLVVRG"
     gene            complement(15650..>17298)
                     /locus_tag="VH419_06695"
     CDS             complement(15650..>17298)
                     /locus_tag="VH419_06695"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HEY7043338.1"
                     /translation="NPVDITDRDRVGMPEIPLTPIDVPGELDRARTALAGMTVTELAA
                     SGEATLKAIVKAFRDENIDVLYLVCHGALLDGSQPRLYLEHADGSTHVVDGRVLVDHI
                     AETPHPPTLAFLCSCQSSGRGDLELQEQSSVDQGALSAFGPMLAASGVPAVVAMQGDI
                     TMETARLFAPAFFEELGRHGIVDRAMSVARSAIRDRERDWWVPVLFSRLKRGRTHYLP
                     EFGDGGDTVFKAMLSKIELPDCTAIVGSGVASEKILPSREDLANSWADVWQMPIASHI
                     RADLAKVAQYLSVQTAQDQPQAELQLYFKKRMREVLSQEGDLDDSQRTDATRLSDLIR
                     TAGASHRRQAGDDDPYKILAALKLPVYITTSWTSLLEDAIEEARGERPDIRTFGWHLT
                     REDVYDEPEPTREKPLVYHLFGTFDDPDSLVITEDDYFAWLTQWIKRVDKDESIPGPV
                     RDSLTNTSLMFLGYGLDDWEFRVLFQSIKSFEGSRLRRKVHIGVQVSPDSNLAIEPEA
                     TQEYLESYFGKEERVQVSIYWGTPGRFLKDLSKRMQRQSP"
BASE COUNT         2774 a         5747 c         5801 g         2976 t
ORIGIN      
        1 gacataacca cgggcaagac gaatcatcga agcgtgatga cgatcaacca gcaacgtgaa
       61 tgcggactcg tccccggcgc ggagccgcgc caccagatcg tcatcggccc accggtcgac
      121 aactgcttgc gcgggaccgg agatggtcgg ctgggtcgcg cggggacgcg gtaacgagcg
      181 cctatcgtac gacgtagcag cctcccggcg gactgccccg agctcggctc ggggtggttg
      241 gtcggcacac accaatgtca ggctcacctt ttggtgaaca gcctttctcc agatggtccc
      301 gtgaggtcgg ccgcgcgtta caccacgaca agagccggct tgcgatccga accagaacgc
      361 acgccgcgac tgagtcgcgc ttcgggcgca agcgcgtatg cgtgcgtgtg cacgacgtgc
      421 cggttgccag gagttgccgg tcacgacgcc tcgtggatgg ctccggagat catgagcgaa
      481 cgcctccgct cctaccggcg tcacgtggcc actggtcggg ccgagcttga gctggtgctc
      541 aattcgacgc caaacattcg ggtgaagaac gctcacgacg cccaggtgcg ttgtgagact
      601 ggcacactct caagacatcg gggatgactt cagactggag gtcgcgatga atgcgaagtg
      661 gtggcgccag ggcggtctgc tcggaatcgc attcgtattc gtctggttgg tcgcggtcct
      721 cgtccggggc gatcctcccg cctacgacca tccggtcggt gagctcaggg cctactggca
      781 tgaccacgga gaccgctacc tggtcgccca gtacgtcgtc gtcctcgccg cgctgaccat
      841 gctcatcccc tacatggtcg cgttcaccac actgctcggg cgggccgagg gcgagccccg
      901 gatctggtca cggatctcgc tgatcggcgg ctcgctcttc gtcgccgtgg cgctcgccgc
      961 tcagtcggcg tgggctgcgc tcgccatcga cgtggaccgg ctcagcgacg acgcggtggt
     1021 caccctgatg cagctcgacc aaggcgccta tcaagcgccc ccgcctctct acggactctt
     1081 cgtcgccgcg gcctccatcg tcatcgttcg aacgcgagcg atgtcgcgct ggcttgggtt
     1141 catggggctg gccatcgcgc tcggaatgct cttgtaccca ctgtcgctgc ttttcgagaa
     1201 ccccgataac gtcttcacgc tgctgtactt cttcgcctat ctggcggggc aggcgtggct
     1261 cctgacaggt gcgatcttca tgctccggat gcgtgaggag gaggccgttc tccgcccttc
     1321 ccggctggca tccccagaac cctagtcagg gagcccccag agcgtcgcgg tgctcgcaca
     1381 tggtcgagcg tctgcgtgct gtgcggcgac atgagcggac gtcagagctg tgtcatcgcg
     1441 cggctcactg ttcgacgcac aatgtgtggc atggatgtcg tggtcgtcgg ggcgggtcag
     1501 gccgggctcg ccgtcagcca cgcgctcacc atccgtggtg tcgaccacgt ggtgctcgag
     1561 gccgatcggg tcgccagcgc ctggcggcac cgctgggaca gcttcacgct ggtcacgccg
     1621 aactggaccc tcgccctgcc cgatgcgccg tactccggtg aggacccaga ggggcatgtc
     1681 agccgcgacc agatcgtcga ctacttggag tggtacgccg ccgcgcacgc gggagagatc
     1741 cgcgtggggg tgcgagtcga ccagatcgcg ccgggtacgg gtggccggtt cgtgctcgac
     1801 acgagcgatg gggtcatgtc gagcgaccag gtcgtggtct gcaccggcgc ctttcgcgta
     1861 ccccacatgc caccgcatgc cggcgcattc ccaccacacg tcaccgtgct cgacgcgacg
     1921 tcctaccgag cgccaggcga actctctgcg gctggggtgc tggtcgtcgg cagcggccag
     1981 accggggtcc aactggccga tgagctgcgc gcgagcggac gcaacgtcac ccttgcctgt
     2041 ggacgcgcac cgtgggcacc gcgtcgtgcg gatggtgtgg acatcatcac ctggttggct
     2101 cggacgtcgt acttcgagga ccgggtcacc gatctgccgc acccgtggtc ccgactggcc
     2161 gcgaatgcgc agctgacggg tgccggcggc gggcacgacc tccacttccg cgtgttgcag
     2221 cagcaggggg tcacgctcgc tggccggctc accggcgtcg acgccggtcg tgccctgttc
     2281 gccgacgacc tggccgattc agtcgccttc ggcgacgccc ggtacgccca cattcgcgcg
     2341 cttgtggccg ccgagctcgg tcccgatgca cccttcctgc ctgacccgga gccgttcgtc
     2401 gccaccgccc cgaccgagct cgacctcgcc gacgtcggta ccgtgatcat cacctccgga
     2461 ttccggccgg accacgcccg ctggatccag tttccggtct tcgacgagct tggcttcccc
     2521 atcgtgggcg acgacctggc caccgcggtc ccgggcctgt tcttctgcgg cgtgcacttc
     2581 ctgcgcaacc gccgctcctc gctcctgtgg ggggtcggcg acgacgcggc ggtcgtcgcc
     2641 gacacgctgg cccgccaaca gtaggccgct gccaccggcg gcatcgtcgc gcgacccggg
     2701 cggcgcgcct atctgcgcac gtctccgagc ctgacggtcc gctacttttc ggagggtgga
     2761 cgagccgatg gagagtcagg gcccttccca gccgcgaacg cccccgatgg ccgaggacga
     2821 tccggttgtg cagtggctgt tgcagggcga cccggcgatc cggtggcagg tgctgcgcga
     2881 ccttgtcgag gcctcggaga ccgaggtggc gacggagcga gcgcgggtgg agcacgaggg
     2941 ctggggtgct cggctgctgg cgctgcgggc tcctgatggc ctgtgggcga acggcgcctg
     3001 ctttcccgat accgcggcgt tcgcagcggc tacggcgcgg gcgctggccg ccggtgagcc
     3061 accgccgcct ttcccgtcgc ccgacgccga accggccgag gcaccgaccg acctttcgcg
     3121 tgccggaggc cagccgtgga ccgcgacgta ccgggtcctg ctcgacctgt gcgacctggg
     3181 cataccgccg gccagtccgg tgatgcagga gaccgcggag ctggtggccc gcaactgccg
     3241 ttgggaatgc gacgggctgc cgttcttcgc cggtgaggaa gactgctgca tcaacgccgg
     3301 cacgattctg atcggtgtct acttgggggt cgacgtcgac ccggtggtgc ggcggctcgt
     3361 ggccgaccag atgccggatg gtggctggaa ctgctgggcc gagacgcgcc cagcgccagg
     3421 atctttcgcc agcaccctgg acgtgatcga cgctctgctg cggtgggagc gccagactgg
     3481 cgggtctgag gaggtccgcc gggctcgccg gaacggagag gagtacttgc tccggcgcaa
     3541 cctcttccgg tcgttacgca cgggcgagtt ggtcaacccg cagtggacgt tgttctccta
     3601 ccccccacgc tggcactacg acctgctcaa ggccgccgag tacttcgccc ggcgtggggg
     3661 cactccggat ccgcggctgg ccgaagcgat cgaacgcgtc cgcgcaaagc gccggcccga
     3721 cgggcgttgg ccgctcgaga acacccatcc cggcgcggtc cacttccggt tcgaagaacc
     3781 cgacggcacg cctagccggt ggaacacctt gcgggcgctg cgggtgctgc gctggtacga
     3841 cgcgcccgcc cactccgaca gggcgagcca ggcgacttga cgacagctgg cgacatcacg
     3901 agctgttccg agtcgtcgtc aacgtcgcat tgagacgtcg cggttgccca cctgcatgtg
     3961 acatggtcgc gcggttgggt ggctaggtgg ctgacgatct ggccgagagc tcagccggcg
     4021 tgtaggccga actcgttgcc gtctgggtcg gcaagttcga ggccgtcatc caggtcggca
     4081 actcgggttg ccccgagtga gagcagtcgc cccgcctcag tgtgggggtc agcggtcaac
     4141 agatcgaggc gctgccggtt ccgcccgcgt ttgggctcga ccggtgggcc gccccatgag
     4201 accttcgtgc caccaagccg tgactggata gaagtctcct cgtcctggtc ccacaccagc
     4261 ggccagccaa gcgcgtcacg ccagaacagg ccgacatctc gggtcccgtc acaggtgacc
     4321 tcaccgagcc ggccggtgcc ggcgaggaac gtgttgtccg gctcgatcac gcacagttcg
     4381 ttaccgccgg gatcggccag cacgacgaac tccgcgtggg gctcttggcc cacgtcgatg
     4441 tggcggccac cgagccccag cgccatctcg acgatccgct gctggtcctc gagactgctg
     4501 ctggtcaggt gcaggtgcag ccggcgcggg ccgatctgct ggatgtcgga ggtgacgaac
     4561 cggagcccga cctgtgtctg atcaccaggc accagcgcac tgccggactc cgcgtggacc
     4621 tcccgtcgca gcagccgagc ccaaaacgct gccaccgcgg acgcatcggc cacatcgaag
     4681 gtcaccgctt ccaaccgcaa agccatccct gcacggtagg acccgctcaa ggacacggcg
     4741 accaaatttc acgtgctgtt ggacccgggc cgcctccgtc aggcacgtga aacggccggg
     4801 ctagcgtgcc gtcagcaaag cggcctggtc gcgcgcgcgc gtgaggcggc gtggtcgctg
     4861 tgatgtcgcc gatggggagc tgaccggcgg gcagcccgat ccgctaccgg gtgatcgtcg
     4921 cctcgacgtc gatcgcttcg agcttggcgg ggttgcggac cgcgtagatg cggctgatcc
     4981 tgccctgctc gaccacgaag ctgatcgccg tgtcgtgacc gtcgctggtg cccatgatcc
     5041 gggccccaac cgaaccgttg agcaacgtag ggaggatcct ggccccgggt tcgaggcggg
     5101 ggaacgggcg gagcaagttg gcgaccttct tcgctcccac cacggggttc accacggcct
     5161 ggaccacccc gccaccgtcg gcgacgagga cgacgtccgg agcgaggacg tcgagcagcc
     5221 cctgcaggtc gccctgggtc acagcggcca ggaaccggtc gacgacctcc tgccgctggc
     5281 tgcgactgac ctccatccgc ggccggcgtg ccgccacgtg ctcgcgggct cgatgcccga
     5341 tctggcgcac cgcgcccgcg gacttgccga ccgcctgtgc gatctcgtcg tacggcgtgt
     5401 cgaacacctc gcgcagcacg aagaccgcgc gctccgtcgg cgtcagcgtc tcgagcaccg
     5461 tgagcatggc gatcgagaca ctctcggcga gctcgacgtc ctcggcgacg tccggggccg
     5521 tgagcagggg ctcgggtagc cactcaccga cgtacagctc acggcgccga gcgttcgaac
     5581 gcagtcggtc gagtgctttt cgggtcacca tgcgtaccag gaatgcccgc gggtcacgca
     5641 cgtcgtcgcg cgcgtcgcca ccgatcgcat cccaccgcag ccaggtgtcc tgcacgacgt
     5701 cctcagcatc agtggccgag ccgagcatct cgtacgcgac ggtgaagagg agcccccggt
     5761 gggtgacaaa cgggtcctcg gccatggtca ctgctacgag gctacggcgg cgggctgggc
     5821 gagcggcttg agcccgcacg ccgcagcgaa gccgtcggac tcgatgccaa gcgcgacatt
     5881 cgaacgcgtc gtgaggttgg cgaacgcgat gaccgcggtg agctcgacca cggcggcgtc
     5941 accgagttgc tgccgcagcg acgccagctg ctcatcggtc actgtcggct cggtcacggt
     6001 catcgcctcg gcgtaggtca gcacctcacg ctcgagaggc gagaacatgt cggactcacg
     6061 ccagcgcggg atctcgcgcg ccttggccat gtcgaggttc ttgttgaaag cctcgaagta
     6121 gttgaagtcc aggcaccagc tgcagccgac ctgtgcggcc actgccatgt gcgcgaacga
     6181 cttgagctgc tcgtcgcacg cgtcccactt ctggaccttg ccgccggcgc cggccatcgc
     6241 catcagcacc ctctggttat gccagtagac accaagcgac gtcggaacct gcccaagaga
     6301 ccgcttcgcc aaccgcttca tcagcatgcc tttgaagccc gtgatctcgg ccgccgggat
     6361 ccgagttgct ccagtcatgt cgtcctcctg tgtgagtcgg tacgacatca agacgacggc
     6421 cgaccgttcg atgtgacatg gcggtcactt gaagcggaca ctcccttggc gcctctgctc
     6481 gcatcggacg aggacgacgt ccaccacctc aggccggtgt gcgtgagcgg ccctctgagc
     6541 cgctacctga agtgatgcat cccagtggta cccatcgatc caagtgtcga agcgggcatc
     6601 ctcgaggggg cttcgggggt cgaacccaca gggtcgagaa ggggctggtg tctgaacgat
     6661 cgctcttggt cgtggtcgcc tctgtcacgc gattcccgtt gctccactgc aacgggggct
     6721 cgcgagctct tggctcacgt cacgttcatg tggaggtgtg aaaccagatc gttgccggtt
     6781 tgtggttcca ggtggaagca tgagatgcgt ggggggatgc ggctgccagg acaggcacct
     6841 cgacccgacg gggggcgcgg gggagctggt gtttggctgg gccggcgtac tggtcgtcga
     6901 cgtcgactac gaggagtttg ccgaccttgt cagtgggcgg ccggtgttcc acgagtacgg
     6961 cggcccgacg tatcgctgga cgcgcggaat cctgcgctac atgcaagggc tgggaagccc
     7021 tttcctgcag tcgcacggcc ttcggttgct gattgccgcg caggatctga tcgaggctcc
     7081 aaccgggtca cacgaggcgt tcacggcgga ggcccgacga ctcgccgagc ctgatgctgg
     7141 tggactgtcg tgcgaagact tgctcgctgc cgcggcggaa gtcgaggtac tgcgattgct
     7201 accacgcgat ccaaccctgg aactgatgtc accggggagc tttcaccgcc tcgttctcaa
     7261 gcaggtgcat cccgatcctg atgcgccagc tcgccgcggt ttcgaagcgc ttgcacgccg
     7321 cattggcgac gacgacgcgt gcgaactgtt cccgatcctg acattcctgg cgttcatcct
     7381 caacgaccca tgcgagacct tcgattcgct cgtccgcatg gcagagctct cggcacagcc
     7441 gttcaaacaa ttgacggcag cacagctgat cgactggctc gggtgggcag atgactacga
     7501 gcgatgctgg gacctcgtag ctgccggcga accggtcgga acgccgtatc tggtcgaccc
     7561 tttgcgcgct gcgatgcggc tgcacggacg ttccgaactg ctcgaagtcc tcgcacggcc
     7621 atcgtcgcat ctcaacgcgc tccccgaggc acaggcccga gccgtgcagc cacccatcat
     7681 cgtgttccca cgcagggacg gtggcgtgca tcaccaccgc aacggagtcg cgctcgatga
     7741 cgtcgactgg gcgcaagagg cgcttgtcaa cctgggccgc tacggcgccg cggagcggtt
     7801 gacggacaat cactcggaga cgaacccgtc ctactgcact cacctcggct gtcctcatca
     7861 tgcggtcggc ttgtgcaacc gctggtatct ggcgccgaca acggcgcagg gccacgacga
     7921 ctgttcgttc ccgccggtct tcgccgccag cgccggcatg tatccggccg agatagtcgg
     7981 gcggttgtcg tgacgagcgg gtcgctcgct gtggtggtga ttgaggatga gccgatagag
     8041 ggcgtggcag accgtgtggc gaggatgccg agcgatgctg aagacgacat tgcaacgggc
     8101 gacctggttg acggacttgc cgccgaccgc atttcgcttc ccaccgagcg cgggttgccg
     8161 gaccttgtgc ggaggatcgt tgggcggctg cgcggccttc aactggtcga cgacctcggt
     8221 cgggttgatt cctccttcga gctgcttggc cttcatgtgc cgccgggcgg gcgtgcggcg
     8281 ctggaaatgc ggcggagcgc aaccgtagat cggcagatct ccgttacggc gatgggcctc
     8341 gggttcggtg gcggtcgaag attcacgatc gcgatggatg acgacatcgg ggagcgctca
     8401 ggttgcatgc gggtgcttca acgggtcgaa ctccacgttc ggcggttcgc gaacgaggcc
     8461 gacggtgccg agccgatcat caggaccgac gtcgttggct ggggagcgcg cgagctgacg
     8521 ctcttggcag actgcccgtt ctgtggcggc gtgggtgagc ccggccccct ggagtacgag
     8581 caggatctcc cgcaggcgtt ggatcttcgc accttcgacg ggcctgtcaa gcgcgagacc
     8641 tcctattcgc tggacggatc gcgaaagacc gatatcggcc tctcgctcgc tgtcccgggg
     8701 gcggctgaga cagtccatgt cgggtttcag cttgaacagc agacacggtt ggagtgcacg
     8761 gccacctaca gcttcccgcc cggacggttt tacgtgccgt atcgctctcg tgacgcgcca
     8821 gccacgctgc cgtactgggc tgtccgttga catgggcgcc ctcgcccgga ccatcgccgt
     8881 tctgccagca ggctgggcgg agcaccagtc cgaccggatc gccgaactcg tgtcgtccga
     8941 cgatgccctc gaggcacgag cctggcggct ctggctggag gccgagtggg ccaagcatgg
     9001 cggcgagttc gggctggccg agcaagcgct cggcgcggcc gatcagcttg caaacgggat
     9061 acggacgcga caactgtggg atctcgagtc gcatgggctg cgtggaaggc tgctccacga
     9121 ttgcggtgaa cccggacgcg cccatgagca catcaattgg gcgatgcaga actggcttcg
     9181 cgtaaggacg gtgctgcaga gtccgaaccc cgacacgggc gactttgcac tcgacctgct
     9241 gcatagcgct cacgcgatgg tttcggcgat tgtcgacccc catgagatcg ccgagttggc
     9301 gaagcttctc gacgcagcac ctttcgaggt gacggcgggg atgtgggcca gcgaccgggt
     9361 gccctcccag accgtggagt acgtggcccg cgcgattgag ctcgaggcga aagtcgactc
     9421 gtattcagct tcgcggcgtc tggccaacga tgtactcgcc tggatgaggt cctgggagct
     9481 cgagtcgtcg gaggcactgc ggttccaggc tggcgtgcgg ctcggtctct cctccgccgc
     9541 gctgggtacg cacgagtttg cgaccgccgt tgcccaggcc gacgacgggc ttgcactcgc
     9601 cggccaactt cccgagggga cggatcgatc aggcgtccag gcagaactga aggccaaccg
     9661 aggaaacgcg cttgcaatgc ttggtcggac aagggacgcc gcggccgact atgacacggc
     9721 aatggtcgcc ttcgacgaag ccggagatca gaccgcggca ctgagcatgc gcttggcgaa
     9781 actgaccgtc cgccacgacg ctggcgagga cgtcacggat gtcgaggtcc aagcaatgat
     9841 cgccgagctc gaggacgagg ttcatcgagt ccccaacagc gggcgtctgc gcggtgagct
     9901 cgagcgaaca cgtcgctggt ggctctcctt gctggcgcag aaggagtcag acgacacgga
     9961 gcgagctgtg ggactcatcg aggtgctccg ggacgaccag ccggtactcc gcaccggcac
    10021 ccgctcgcaa gatcacgtgc tggatctgct ctgtcgcgcc gctacggtgt tacgcgcacg
    10081 actgcgcgca ctcgccgaca cggtggtcgt tgtcctcgag cccggtatcc gcacgggcga
    10141 tcgcctgtat ccgcctgttt tcctggtcat ggcaggcggc gaatcgaccc tggtcccatc
    10201 gggagacgcc gccggacgcc tagcggagct cggccgaatt gcctcgatcg agcgagaacg
    10261 acttcttacc ggcgagacaa ccctcaactc accggcatca ggcaggctga ccgaggccgc
    10321 cgtggccgcc tggaaggcga tcccgagcgt cgtcaccgag cgggtgctgg ccgccgagac
    10381 ggtgctctac atgccgagcg gcgtcaacgc cattgacgcc ttgcccttcg aactcctgcg
    10441 gcacgaggcg ggctggctgg gcatgacgca cgtcgtcgcg cgcgcgccgt cgttccagtt
    10501 gctggagcag atgctgacgc cgaacgcacg tgcacatacg aacgattcgc gcgcggtcat
    10561 cgcgaccgta cgccaggaca gccagctagg cgtgctctcc gaagccctgc gggaggccga
    10621 catcgtagag cgcgccgccg aatacatggg gctcacagcg gatcgtcggg aaacgttgga
    10681 tacagacgcg ttactcgacg tactcaggcg caacgcgctc gtgcactaca tcggacacgg
    10741 ctttgccagc gagatcggcg aagtgctccc gctgacctcg gacaccggcg tgtacgttga
    10801 tcaactcagc gaccacgacg gcatgcccag cccgctcgtc ttcttcgacg cgtgcatgct
    10861 tggacgggtc cgccacttgg agggcgggcg gcaacgcggc tggacacttg cgctgcttga
    10921 acgcggcgcg cccgcggtca tcggcgcgtt ggctccggtg cccgactcgg catgttcggc
    10981 ggtggccaag gcgttctatg gcgccgtgct ggacgcacct gtgggtgtgg ccatgcgaaa
    11041 tgcgcgggcg ggcctcgagc aagcaggcct ccacccactc atcgcagcgg cctacgttgt
    11101 ccacggcgac cccagtgcac ggctcacacc aggacaccgg ggaggcacgg cgagccgcgt
    11161 atgccgttgg ccttcgctcg ccgcacggtt tctggcaacg gaacatcctg acgaccgcgc
    11221 ggagattgtc tctgcgagct gtgtcccagc ggcagtggcg agctgggcaa acggtgttga
    11281 tgtctccccg gctgagttcg cctccgcagt cgaaatgacg atcgcagagg accccgaggg
    11341 ggccgccatc tgccgtatct tgctggcgtt gaaacggttt gaccagaacc cgaacgacga
    11401 tgacgagctc gacgttgcat ggctcgtcgc cgaagccgtg ggggaccgct acgcccaact
    11461 gcatctgatc gaccgatacg caccggcgct gattcgccgc catcccgacc ttcgggcgca
    11521 cctgccctcc gagactcggt tctcgctcaa cgcgctcggc ggtggccgcg gcaggctggc
    11581 tggtcttgcc gagaggctgg cccacgtctg ctgacaaagc cggcttcggc cggcgatcca
    11641 gaccgtccac tgtggacccc ggaagcccca cgctccaacg ttcacgtaga accgcggccg
    11701 gacgtcttcg gcacatgctt cgagatgtac aagaccttgc tggcgcatgc ggtcaggtgc
    11761 gagacctcct tggtttcagc cgacggctcg acacatcatc cgcccatcgg cagagcccgc
    11821 ccgcggaggg atggggtgtg tttcgtctgg gagtagcagg cgggttcgct taccccttga
    11881 ccctggtcaa cgtgacgtga ccgcggcgac acttgacgcc catgagcgcc tacttcggac
    11941 tcgacctcga cagactgttg ggcttgtggt ccgtgccgcc ggccgaccgc catgacgcct
    12001 acgcggactt cgccgcgctg tacgaagatc ccgtcacgat caacgactca ccgacgcccc
    12061 tgcaggacct cgtcgcccgg gcccaggcga tcaatgaggc attcagcgac cagcagagcg
    12121 aaattctcga cgtcgtcgcg gctcgaagca agctggctgt cgcgttccgg cgtcatgcca
    12181 cccacaccgg tacgtggagc agcccgttgg gcgatcttcc ggcgacgcac gaacgggtga
    12241 ccatcatggg tctcgacatc ttcaccatca ccgatggtcg gatcaccgcg atccgggttc
    12301 tcggtgatga cctcgctgta ctgctcactg cagctgggcg ccgactttga acctgcgggc
    12361 ccggccccag gggcgcactt gcatgagtct tcccgcattc ggctcgctca tcgcatgacg
    12421 ggtcagcagc cgattggcgc ggtgcgccaa acgcggtgct gcttgttcac atccgcaccc
    12481 aagccgaccg gtcgtcacgg agaagggcca ggtcagaggg ttatcgctgg tgggaggtgt
    12541 ggccagggcc ggggtcgaac cggcgacctt ccgcttttca ggcggacgct cgtaccaact
    12601 gagctacctg gccgtacgcc gtgcgctgga ccgttcggcg cgaccgaaac cctacctcag
    12661 caggtgggta cggcccgaat ccgccgccct cagcaaaccc gaggagccga cagcggatca
    12721 ggtgcgcagg cgaaccgcag ggtcgccgat gacgacgtag cttcgcgcat cgttcaccgc
    12781 ggtccacatc cgtgcagtct cgacgtcgtc gagtcgcagc ccctccgtac gtgcttcgtg
    12841 caacatgtcg tgcaacgctg tcgacagcga cgagtagcgg tcgtcgaaga actccatcgc
    12901 gtgtccgacc cgccagccgg ccatgagggc ttgcaggcag gacgcgaaca cgtccgtctg
    12961 tgatccgaca ccgggcgcga ggaacgagta gccccaggcc cgctcgacgt ggccgacgaa
    13021 ggccaacgtg gagctgtggg cgtgggtgag aagctgctcg gccagcttcg agacgaacgg
    13081 cacctgcgtc agctcctgag cggcggcccc gtcacctgga cgcgagaact cgtcgcggtg
    13141 tggcgtaccg gcgccgaagc acgcgaaggt catcaccagc tcggtgttca cggcgtcgag
    13201 agcctcgacg tccgagccgg cgacgaagca gtccggtggg atgggctcct gcccgttgtc
    13261 cgcggggccc gaccagtcct ggcagacgag tgcgccttgc tcgtcgcgtt gccgctcgtc
    13321 gtcggcgtcg aaccccagcc cgtgggacgc ggtgaagagc agcgcggggg cgtcatcatc
    13381 ggagacgagt tggagtagcc gatcctttgt gcacgcaccg ggtgggagga cgtcgatgtc
    13441 ccactcacgg acatcgtgct gaagcgccgc ccccaacggt gcgaccagct ccgtcgcgct
    13501 caagatcgtg gcgcggtcgt cttcggccgc cggaccgaag aatcgactgc gacgagtact
    13561 gggccggtcc gtcgcctctg cggccaccac agccgagaca tagcgttcga gtcgctcgtg
    13621 gtcgaagctg agccggccga cggcgtactc gacgtcgagc tggtactgga aggtgaagcc
    13681 gatctgttcc ggcccaccga tcaacaagag gtaatacggc agccggacgg gattgacggg
    13741 catccctggc ccgatgccgt tgcgcgcgag gaacgctcgc ttgtcctcgc cgggcgccag
    13801 gccgtccgga ccgatgaact cgcggtaccg ctcacccgcc tgctccgcac gaagctcacg
    13861 cagcggtgcc agcgtttcca gtacgtcggg tggggtgtcc tgggccgcga cgagcgccca
    13921 acctgtcgac gcaaggtcat caaggtcgtc gacgccgaag accacgccga aggcaggctc
    13981 ggcgtcgcgg tatctgagct cgtcgagctc gtcagcgtgg tgtgtcgccc caccccgaag
    14041 cccggccgcg agcgaacgac gggtcgtcgg aggccacaga tactcgccgg tcgacccgtt
    14101 gagcccgttg aagacgacat cctccgggtc ctcagccaga cggtcgctca ccctctcacg
    14161 accagcagaa caaggatgat gttgaggcag atcgagagcg tcaggaacaa cgtcgtgcgc
    14221 tggagcgact tggacagccc cgcgcgcatc ttcgccagcg cggcttgttg ctgtgagagc
    14281 tcgtcggagg tccggacccg ctccgcggac tcttcgacga acgccttctc gtacgacgtg
    14341 ccccggtcgc ccaacgcagc cagctgccgc tgcgcgggct cgagccagct tcgctcgagc
    14401 aggtagttcc ggtcgtggcc gcggcgatgc cacttgtgcg cattcactcg ccagggtgaa
    14461 agggttgtac ggcgccagga ggcgttgtcc gacaggacag gcttgatcag ccggtcgtgc
    14521 gagagctccc accaggcttc cgcgcgctgg tcaccgcgga tcaggtagcg atcctgcagc
    14581 gccgccagga cgtcgcttcg gtcgtcgagc tccggtcccg tccgtgtctg tgacctgaac
    14641 tccgcctccg tgatcagctc gtcctcgaac cagtctcgaa tcgcgcgctg ccgctcgatg
    14701 tctgcgccgg ccacctcgac gaccgcgtcc gcgtagtacc gccgcagcgc ccggttgacg
    14761 gagccgaatc gctcgacgtc agccgcggtg atctccgccg actggcgcga gtcgtcgctg
    14821 tcgagccggc gccacagccg gtggcacacg acctgcagca tgacgggctc gacgtacggt
    14881 gccctgtcca cccgaaccga gtcgtcgggg tcttcgacct tgatctttcg taggtcgtcg
    14941 agaagcgcgt ccgttgcctc cggaaggaag gagacgttgc gcgctttcgc cggctcgacc
    15001 atcgcacgtt cggcagcgct gacactcagg aagtccagtc ggtacgtcgt ccggaggcgt
    15061 cccggcagca gcggcaggaa gcggtcgagc ccgcccatgt agtcctcacg catcgcgagc
    15121 agtgcccagc ggcgcggatc gtcgagcgct tctcccagct gtcggaagaa gagtgtctgg
    15181 gcgtctcggt tggcggggtc gagggtgagc acctcctcca tctggtcgat gatgatgagc
    15241 tggtcgcgag ggtcgtccga tcgctcacgg cgcagcgcca gggcgtcgct caccgtcatg
    15301 gagtccagtg cagacacgcg cgtctcgtgc ccttggagca ggccgttgat gacgctacgg
    15361 acaaagcgtc ggtccgacga agcggagtcc ggtggcggtt cgtggacacg cagcgccgag
    15421 aagtactgct tcttggccga gcagatctgg aagtcctcct tgaggaaggg cacgagaccg
    15481 gcctggacga gcgaggtctt gcccgctccc gacggtgagt ggagcagcac gacccggccg
    15541 gagatcagga gattcgccag gttcttcgtc tcgcgctcac ggccgtagaa gatgtcggtg
    15601 tcgcgaaacg gccgcggtcc gacgaacgga ttctcctgaa gtttgagcat cagggactct
    15661 gcctctgcat gcgcttggac aggtccttca ggaagcggcc tggcgtgccc cagtagatcg
    15721 agacctgaac ccgttcctcc ttgccgaagt acgactccag gtactcctgg gtggcttccg
    15781 gttcgatggc caggttgctg tccgggctca cctgtacgcc gatgtgcacc ttccgacgca
    15841 gccggcttcc ttcgaagctc ttgatgctct ggaagaggac gcggaactcc cagtcgtcga
    15901 gtccgtaccc gaggaacatc aacgacgtgt tcgtcaggga gtcacggacc gggccaggaa
    15961 tgctctcgtc cttgtccacg cgcttgatcc actgcgtcaa ccaggcgaag tagtcgtcct
    16021 ctgtgatgac cagggagtcc gggtcgtcga acgtcccgaa caagtggtag acgagcggtt
    16081 tctcccgcgt cggctccggc tcgtcgtaca cgtcctcgcg tgtcaggtgc cagccgaacg
    16141 tgcggatgtc cggccgttcg ccgcgagcct cttcgatggc gtcctcgagc aggctcgtcc
    16201 agctggtcgt gatgtacacg ggaagcttga gcgcggccag gatcttgtag ggatcgtcgt
    16261 cgccggcctg gcggcggtgg ctggcgccgg cggtccgaat caggtcggag agcctcgtgg
    16321 catcggtccg ctgcgagtcg tcgaggtcgc cttcttgcga aagcacctcc cgcatcctct
    16381 tcttgaagta cagctggagc tcggcttgcg gctggtcctg cgcggtctgc acgctcaggt
    16441 actgggcgac cttcgccagg tcggcacgaa tgtgcgatgc aatcggcatc tgccagacgt
    16501 cggcccaact gttggcgagg tcctcccggg acgggaggat cttctcgctc gcgacgccgg
    16561 aaccgacgat cgcggtgcag tcggggagct cgatcttcga cagcatcgcc ttgaacaccg
    16621 tgtcgccacc atcgccgaac tcgggcaggt agtgcgtccg tccgcgcttg agacgcgaga
    16681 agagcaccgg aacccaccag tcgcgctccc ggtcacggat cgcactacgg gcgacggaca
    16741 ttgctcggtc gacgatgccg tgccgaccga gctcctcgaa gaatgccggc gcgaacagcc
    16801 gcgccgtctc catcgtgatg tcgccctgca tggcgacgac cgccggcaca ccggacgccg
    16861 caagcatcgg cccgaaggcc gagagcgcac cctggtcgac gctcgactgc tcctgaagtt
    16921 cgaggtcgcc gcggccggac gactgacagc tgcagaggaa cgccagcgtg ggtggatgcg
    16981 gcgtctcggc gatgtggtcg acgaggacac gaccgtcgac gacgtgggtc gatccgtcgg
    17041 cgtgctccag gtacagccgc ggctgcgaac cgtcgaggag agcaccgtgg cagaccagat
    17101 agagcacgtc gatgttctcg tcgcggaatg ccttcacgat cgccttcagg gtggcctctc
    17161 cgcttgcagc cagctcggtc acggtcatgc ccgccagcgc cgtacgggcg cgatcgagct
    17221 caccaggtac gtcgatgggc gtcagtggga tctccggcat gccgacgcgg tctcggtcgg
    17281 tgatgtcgac cgggttcg
//
LOCUS       DATBFZ010000220         5951 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_5980891, whole genome shotgun sequence.
ACCESSION   DATBFZ010000220 DATBFZ010000000
VERSION     DATBFZ010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 5951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5951
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5980891"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2212)
                     /gene="purL"
                     /locus_tag="VH419_06700"
     CDS             complement(<1..2212)
                     /gene="purL"
                     /locus_tag="VH419_06700"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012924214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HEY7043339.1"
                     /translation="MTAKPDETFDTVDRAAGSPDIQQPWRELGLRPDEYGQIREILGR
                     RPTSSELAMYSVMWSEHCSYKSSKVHLRQFGEDLTDEMRHHLMVGIGENAGVVDIGDG
                     YAVTFKVESHNHPSYVEPYQGAATGVGGIVRDILSMGARPVAVMDPLRFGPLDADDTH
                     RVLPGIVAGIGGYGNCLGLPNIGGETVFDPSYAGNPLVNALCVGVMRHDQLHLARASG
                     AGNTVILYGARTGGDGIGGVSVLASETFDHGGPTKRPSVQVGDPFMEKLLIECTLELF
                     AERLVAGIQDLGGAGLSCATSELASAGDGGMHIWLDRVPLRDTSLAPEEILMSESQER
                     MMAVVEPANVDRFLAICAKWDVDATVIGEVTEVSEAERQSGGRLVVEWHGQTIVDVPP
                     RTVAHEGPVYQRPFARPGWQDELQADDSRSLDRPKSGEQLRETVLRMAASPNLCDKSW
                     VTDQYDRYVQSNTVLASPEDAGVVRVDEETGLGVAVATDCNGRFARLDPYVGAQLALA
                     EAYRNVAASGARPVAVTDCLNFGSPEDPDVMWQFAEATRGLADGCRELGIPVTGGNVS
                     FYNQTGQTAILPTPVVGVLGVIEDVRRRIPIGFASDGLAIHLLGSTSDELDGSEWAHV
                     VHQHLGGRPPEVDLAAERRLAEVLVAASRSGMAQAAHDVSDGGLAQALVESCLRHGVG
                     VRVSLPDELDPFVALFSESSGRAVVAVAIAEVAAFADLCTAYDVPCRRIGVTGTR"
     gene            complement(2308..3231)
                     /locus_tag="VH419_06705"
     CDS             complement(2308..3231)
                     /locus_tag="VH419_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007079508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HEY7043340.1"
                     /translation="MAAPDLLRIHPGVAEALSARQGVVALESTIISHGLPADSSADVA
                     RQIEAAVRASGAVPATIALLDGVVRIGLDDQALERVATDPAVVKASIRDLGPVLATGM
                     AGATTVASTAYLAHRVGIDVFATGGLGGVHRGAQESWDESADLAALAQVPITVVCAGV
                     KSILDVPATLERLESLSIGLVGYRTDVFPGFYVRDSGQPVPWRLDDVEAIAETVRQRH
                     ALGLQQALVVANPIDESAEMEKVLHDTVVTSGLRALSEAGVRGKDVTPFLLAHFHEQT
                     HGRSLAANIDLVLSNARVAGQIAVAASRTAD"
     gene            complement(3327..3737)
                     /locus_tag="VH419_06710"
     CDS             complement(3327..3737)
                     /locus_tag="VH419_06710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043341.1"
                     /translation="MNRITKMVPAAIGISGLALASLAAVGSAPASAAATCQLTVHSNK
                     VLNLEEDNGTDSIFFKLGNNRTPTLNYALGDKQVNIGSETFQGSIDLKVFEKNGNQRT
                     LVDRLNNIPCQNSPVQVDDATDGDAIYRVRWSVQ"
     gene            complement(4101..4901)
                     /locus_tag="VH419_06715"
     CDS             complement(4101..4901)
                     /locus_tag="VH419_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043342.1"
                     /translation="MNTLDELVTAVLQDEAQDAPRSEPFVTRVLGDAPRSARRSRPRW
                     AAPAAAAAVVGLVAAAAIVASLWPDHGSADGATTPATTTSAPSLRTIPPGHAGRLPGW
                     IDLSWASVHIEMDDSASRGGFWWRSWSVGCSASVLSTMESGDFACAGPPGERPRYEVL
                     VAEGDAPTNTVNARINWGGPLRPSRITGPFDRALCGGPCYQNVGGQTSKVSSTTAQVR
                     YDCPGVCGRPTPRTLTVIDFPEQDAHVTILSPPGERLNWLTDYIYVDR"
     gene            complement(4898..5488)
                     /locus_tag="VH419_06720"
     CDS             complement(4898..5488)
                     /locus_tag="VH419_06720"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigE family RNA polymerase sigma factor"
                     /protein_id="HEY7043343.1"
                     /translation="MRDQRFEQFVRVQSAGLLRLAFLLTRDHRDAEDLVQEVLLSALR
                     KWDTVTAADRPDAYVRRMLVNNHISSHRRSASRELPLGADLVVMGDPHSTERLDEIDA
                     RSWAANVMGQLPRRQRAVLVLRYYERLDDVEIADILGVARGTVRSLAARAFDALRRHP
                     ELMHYADPPERDLSLPTPQAASVISPVTMLDVEGRS"
     gene            complement(5633..>5951)
                     /locus_tag="VH419_06725"
     CDS             complement(5633..>5951)
                     /locus_tag="VH419_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043344.1"
                     /translation="EPPEYEALVSPGNGAIPRDSDWLRLTTKVDKTYHSPTAWEASPA
                     GHPAWVRTVGVSVRYDCGTGCHQWADRTLAVISYPGFYAQVMILAESPSEIDDIAEYI
                     YVE"
BASE COUNT          967 a         2027 c         1988 g          969 t
ORIGIN      
        1 cacgagtccc ggtgaccccg atccgccgac aggggacgtc gtacgccgta cacaggtccg
       61 cgaacgccgc cacctccgct atcgcaacgg caacgacggc tcggccgctc gactccgaga
      121 acagcgccac gaacgggtcc agctcgtcgg gcagtgagac tcggaccccg acgccgtgcc
      181 gcagacagga ttcgacgagc gcctgcgcga gaccgccatc ggacacgtcg tgcgccgcct
      241 gcgccatacc gctcctcgat gccgccacca ggacctccgc cagtcggcgt tccgcggcca
      301 ggtcgacctc cggcggtcgg ccgccgaggt gctggtgcac gacgtgggcc cactcagacc
      361 cgtccaactc gtccgacgta ctgcccagca ggtggatcgc cagtccgtcc gacgcgaagc
      421 cgatcggaat acgccgccgg acgtcctcga tgacgcccag caccccgacg accggcgtcg
      481 gcaggatcgc cgtctgcccg gtctggttgt agaagctgac gttcccaccc gtcacgggga
      541 tgccgagctc gcggcagccg tccgcgagtc cgcgcgtggc ctcggcgaac tgccacatca
      601 cgtcgggatc ctccggcgac ccgaagttca ggcagtccgt gaccgccacc ggacgcgcac
      661 cggacgcggc gacgttgcgg tacgcctccg caagcgccag ctgcgccccg acgtagggat
      721 ccagccgagc gaaccggccg ttgcagtcgg tcgcgaccgc aacaccgaga cccgtctcct
      781 cgtcgacgcg gacgacgccg gcgtcctcgg gcgaagcgag caccgtgttg ctctgcacgt
      841 accggtcgta ctggtcggtg acccacgact tgtcacacaa gttcggcgac gcggccatcc
      901 ggagcacggt ctcgcgcagc tgttctccgg acttcggcct gtccagcgag cgcgagtcgt
      961 cggcctgcag ctcgtcctgc cagccgggcc gggcgaacgg tcgttggtag acgggacctt
     1021 catgtgccac cgtccggggg ggtacgtcga cgatcgtctg gccgtgccac tcgacaacga
     1081 gcctgccacc gctctggcgt tcagcctcgg agacctccgt gacctcaccg atcacggtcg
     1141 cgtcgacgtc ccacttcgcg cagatcgcga ggaagcggtc gacgttcgcc ggctcgacga
     1201 cggccatcat gcgctcctgc gactcgctca tcaggatctc ctccggagcg agcgaggtat
     1261 cacgcagcgg cacccggtcg agccagatgt gcatgccgcc gtcaccggcg ctggccagtt
     1321 ccgacgtcgc gcacgacagg cccgcaccac ccaggtcctg gatgccggcg acgaggcgct
     1381 cggcgaagag ctcgagcgtg cactcgatga gcagcttctc catgaacggg tcgccgacct
     1441 ggacactcgg acgttttgtc ggaccgccgt ggtcgaacgt ctccgacgcc agcaccgaga
     1501 cgccgccgat cccgtcgccg cccgtcctgg cgccgtacag gatcaccgtg ttcccggcac
     1561 cgctggcccg cgccagatgg agttggtcgt gccgcatgac gccgacacag agcgcgttga
     1621 cgagcggatt gccggcgtac gacgggtcga acaccgtctc gccaccgatg ttgggcaggc
     1681 cgagacagtt cccgtagccg ccgatgccgg cgacgatccc cggcagcacc cgatgcgtgt
     1741 cgtcggcgtc gagcgggccg aagcggagcg ggtccatcac ggcaacgggt cgagccccca
     1801 tcgagaggat gtcgcggacg atgccaccga cccctgtcgc tgcgccctgg tacggctcga
     1861 cgtacgacgg gtggttgtgg gactccacct tgaacgtgac ggcgtacccg tcaccgatgt
     1921 cgacgacgcc ggcgttctcc ccgatgccga ccatgaggtg gtgacgcatc tcgtcggtca
     1981 ggtcctcgcc gaactggcgc agatgtactt tgctcgactt gtacgagcag tgctcgctcc
     2041 acatcacgga gtacatcgcg agctcggacg acgtcggccg acggccgagg atctcccgta
     2101 tctgcccgta ctcgtccggt cggagaccca gctcgcgcca cggctgctgg atgtcgggcg
     2161 acccggcggc gcggtcgacg gtgtcgaagg tttcgtcggg cttggcggtc accgcaaact
     2221 ggctccggac tctctcgttc gacgggcggg gtcaacgcta ccgcccgtcg caggcgagaa
     2281 ggatcgacgc acctggcttc cgagcgctca atcagcggtg cgactcgcag ccacagcgat
     2341 ctggccggcg acacgcgcgt tcgacaacac caggtcgatg ttggccgcga ggctccgccc
     2401 atgcgtctgc tcgtggaagt gcgcgagcag gaagggcgtc acgtccttgc cgcgtacgcc
     2461 agcctcggag agcgccctca gcccagatgt caccaccgtg tcatggagga ccttctccat
     2521 ctcggcagac tcgtcgatcg ggttcgccac gaccaaggcc tgctgcagtc ccagagcgtg
     2581 gcgctgccgg accgtctcgg cgatcgcttc gacgtcgtcg agccgccatg gcacaggctg
     2641 gccggagtcg cgaacgtaga acccggggaa aacgtcggtc cgatagccga ccaggccgat
     2701 cgacaatgac tcgagtctct ccagcgtggc agggacgtcg aggatcgact tcacaccggc
     2761 gcacacgacc gtgatcggca cctgcgcgag cgcggcgagg tcagcggact cgtcccagga
     2821 ctcctgcgct ccgcggtgga cgccgccgag ccccccggtc gcgaagacat cgatgccgac
     2881 ccgatgcgcc aggtatgccg tcgatgccac tgtcgtcgca cccgccatgc cggtggccag
     2941 aaccggcccg aggtcgcgga tgctggcctt gaccacggcc gggtcggtgg ccactcgttc
     3001 gagtgcctgg tcgtcgagac cgatgcggac gactccgtcg agcaacgcga tcgtcgcggg
     3061 cacggctcca ctggcgcgga cggcggcttc gatctgccga gccacgtcgg cgctggagtc
     3121 cgccgggaga ccgtggctga tgatcgtgga ctccaacgcg acaacgccct ggcgggccga
     3181 caacgcctca gccacgcctg gatggatgcg caacaggtcg ggagcggcca caaccagacc
     3241 ctagggcccc tccgcggacg cagacaaaag gctgggcgcc gacctcttgg tcgacgccca
     3301 gcccagatct gtccttcgcc tcgttgctac tggacgctcc acctcacgcg gtagatggcg
     3361 tcgccgtcgg tcgcgtcgtc gacctgcacg gggctgttct ggcacgggat gttgttcagc
     3421 cggtccacga gggtgcgctg gttgccgttc ttctcgaaga ccttgagatc gatggagccc
     3481 tggaaggtct cggacccgat gttgacctgc ttgtcaccca gcgcgtagtt gagcgtgggt
     3541 gtccggttgt tcccaagctt gaagaagatc gagtcggtgc cgttgtcctc ttcgaggttc
     3601 aggaccttgt tcgagtggac cgtcagctga cacgtcgcgg ccgcggacgc cggtgcacta
     3661 ccgaccgccg cgagcgatgc gagcgccagg cccgagatcc cgatggctgc tggaaccatc
     3721 ttcgtgattc tgttcatggt gtttccccct gtgtggtggt tcgagcctga tgccctcccc
     3781 cggtgcatcg actatggatc cgctgggtag gcgcctacat cgacaggcga tgcctagatt
     3841 cgtgctcgag gcgcctcaac ttccgtggtt ggcgacttag cacgccagcc actgaccgag
     3901 ccggcggctt gcgatgttca catccgagac ccactgtgcc catcggaggt ttgcgatgtt
     3961 tacacgccgc ttgggctgta aacatcgcaa accaccacgc gcctacttat cggattgatg
     4021 aggtatgcac cgaggccggt tcaggcgtgg cctagagata cctgagaacg cccgcttccc
     4081 cggggaagtc gacgatcgga ctagcgatcg acgtagatgt agtcggtgag ccagttcagt
     4141 cgttcaccgg gcggactgag aatcgtgacg tgggcatcct gctccgggaa gtcgatcacc
     4201 gtcagtgtgc gtggcgtcgg ccggccgcag acccctgggc agtcgtagcg gacctgtgcc
     4261 gtcgtcgacg agaccttgct cgtctgaccg ccgacgttct ggtaacacgg cccaccgcag
     4321 agcgcacggt cgaacgggcc agtgatccgg ctgggccgca acggcccgcc ccagttgatg
     4381 cgggcgttca ccgtgttcgt gggggcgtcg ccttcggcga ccagcacctc ataccgcggg
     4441 cgttcgccgg gagggccggc acaggcgaag tcgcctgatt ccatcgtcga cagcactgac
     4501 gccgagcatc cgacgctcca ggatcgccac cagaagccac cccgggaggc gctgtcgtcc
     4561 atctcgatgt gcaccgacgc ccacgacagg tcgatccacc cggggagccg tcccgcgtga
     4621 cccggcggga tggtccgaag tgacggggcc gacgtggtcg tggcgggtgt ggtggcgccg
     4681 tcggcggagc cgtggtccgg ccacagcgaa gcgacgattg cggctgcggc gaccagcccg
     4741 acgacggcgg cggctgccgc cggagcagcc cagcgtggcc gcgagcggcg ggccgatcgt
     4801 ggtgcgtcac cgagcacgcg ggtcacgaac ggctcggaac ggggtgcgtc ctgcgcctca
     4861 tcctgcaaca cagcggtgac gagctcgtcc agcgtgttca tgatcgacct tccacgtcca
     4921 gcatggtcac cggtgagatg accgaagcgg cctgcggggt ggggagcgag agatcgcgtt
     4981 cgggtgggtc ggcgtagtgc atgagctcgg ggtgtcggcg cagagcgtcg aacgcgcgag
     5041 ctgccaggct gcggacggtt ccgcgggcga caccgagaat gtcggcgatc tcgacatcgt
     5101 cgagacgttc gtagtagcgc aggacgagga cagcacgttg tcgccgcggg agctgtccca
     5161 tgacgttggc cgcccaggac cgggcgtcga tctcgtcgag ccgctcggtg ctgtgggggt
     5221 cgcccatcac gaccaggtcc gcgccgagcg ggagctcacg gctggccgaa cgacgatggc
     5281 tgctgatgtg gttgttcacc agcattcgac gtacgtaggc gtccgggcgg tcagcggcgg
     5341 tcacggtgtc ccacttccgc agggctgaga ggagtacttc ctgaaccagg tcctccgcgt
     5401 cgcggtgatc gcgcgtcagc aggaaggcca ggcgcagcag accggccgac tgcacgcgca
     5461 cgaactgctc gaaccgctgg tccctcatgg gccgggccat ctgttgccct tcacaggaag
     5521 tacgacgcca cgaggctgtc gaactgctgc ggtctcgtac tgcgaatctc caaaggctcc
     5581 gatccgcgcg actgcgccag acgggagctt cccgatgtta ccgaacgccg tgtcactcga
     5641 cgtagatgta ttccgcgatg tcgtcgatct ctgatggact ctcggccaaa atcatgacct
     5701 gtgcgtagaa cccggggtag gagatcaccg cgagtgtgcg gtcagcccac tggtgacagc
     5761 cggtgccgca gtcgtatcgg acactgacgc cgaccgttcg aacccacgcg gggtgcccgg
     5821 cgggcgacgc ctcccaagcc gtgggcgagt ggtaggtctt gtcgaccttc gtggtcaacc
     5881 gcaaccaatc ggagtcgcgt gggatggcgc cattgcctgg ggacaccaat gcttcatact
     5941 caggtggttc c
//
LOCUS       DATBFZ010000221         3565 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_1455019, whole genome shotgun sequence.
ACCESSION   DATBFZ010000221 DATBFZ010000000
VERSION     DATBFZ010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 3565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3565
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1455019"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..718)
                     /locus_tag="VH419_06730"
     CDS             complement(<1..718)
                     /locus_tag="VH419_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009212814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY7043345.1"
                     /translation="MSEKSAGGDYDVSAIQVLEGLEPVRKRPGMYIGSTGLAGLHHLV
                     WEVVDNSVDEAMAGYCDRIVVTLQPDGGCRVEDNGRGIPIEPYPTGPHKGKSAVEVVL
                     TVLHAGGKFGSGGYKVSGGLHGVGVSVVNALSERLIVEIDRDGSRYRQEYSKGGKPQG
                     KLERVGPTPARGRQSGTTVVFWPDPTVFSSEGTEFHVRTVLERLQTMAFLNKGLEIRF
                     VDERPGRETSTTYLYKGGIVD"
     gene            complement(957..1262)
                     /locus_tag="VH419_06735"
     CDS             complement(957..1262)
                     /locus_tag="VH419_06735"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HEY7043346.1"
                     /translation="MTASDEPRPLQESVDQLVRSLRGTSARTLAGVFASWDDAVGPQI
                     AAHAQPASLVDGCLIVDVDHPTWATELRYLEHDVLERLRTVAGVDGVVRIEVRVRRR"
     gene            complement(1259..2338)
                     /locus_tag="VH419_06740"
     CDS             complement(1259..2338)
                     /locus_tag="VH419_06740"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEY7043347.1"
                     /translation="MLVRRLELVDFRNYEVAEVELARGFTAVTGANGEGKTNLVEAVA
                     YLATLESFRGAAPAALVRDGCERAVVRGEVEQDDGRLVAVECELGLTGRSRVQVNKQR
                     LQRTSDLLGVLRVSVFSPDDLALVKDAPGVRRRFLDDTLVALHPRHDRLRREVERVLR
                     QKAALLKQSGGRLGEDTTFTLDVWDARLAEAGDAIGQARGELVDALAPEVARAYDDLA
                     SRPSDVTLGYEPAWRAAGLAEALRVARADEVRRQVCLVGPHRDELQLTVGGLPARTHA
                     SQGEQRTLALALRLGAHRLIAERTRTTPVLLLDDVFSELDEQRGAALLTHLPVGQIVL
                     TTAGPLPPGADVERILRVERGTVCA"
     gene            complement(2342..3436)
                     /gene="dnaN"
                     /locus_tag="VH419_06745"
     CDS             complement(2342..3436)
                     /gene="dnaN"
                     /locus_tag="VH419_06745"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEY7043348.1"
                     /translation="MKFRCERDVLGEALSTAARAAQGRAGSQPVLAGVRLVLTGDELE
                     ISGTDLELTIQTTVQVSGERDGSSVLPGRLTAEAVRTLESGAVTVDASSDHVDITSGR
                     SSFSVRPFAVDDFPRLSIPSGASVTLPASSFGQAVSQVERAASKDESRPILTGVLLAA
                     EEGGLRLVATDSYRLAVCDLPEVSVLASGQKVLLPSRALRELERVLPNSGDVTLRLAE
                     RDATFELDGTRLTTRLIEGEFPNYRQLIPSSYPNRLVIARDPLLEAIKRVRVMATDAT
                     PVRLNIETDEVRLNTVNQDQGTADAVVEARFEGTPLTVAFNPQYLFDGVEAAAGAEVV
                     LDTLDALKPAVVRPVDREDYLYLLMPVRVP"
BASE COUNT          583 a         1258 c         1164 g          560 t
ORIGIN      
        1 aatcgacgat gccccccttg tagagatagg tggtcgaggt ctcgcgaccc ggtcgctcat
       61 cgacgaaccg gatctccaac cccttgttca ggaaggccat cgtctgcagt cgctccagca
      121 cggtgcgcac gtggaactcg gtgccctccg agctgaagac cgtgggatcc ggccagaaga
      181 ccacggtcgt tcccgattgg cgtccccgcg ccggcgtcgg cccgacgcgc tccagcttcc
      241 cctgcggctt gccacccttc gagtactcct gccggtagcg cgagccatcc cggtcgatct
      301 cgacgatcaa ccgctccgac aacgcgttca cgactgagac gcccacgccg tggaggccgc
      361 ccgagacctt gtagccgcca ctgccgaact tgcccccggc gtggaggacg gtcaggacga
      421 cctcgaccgc gctcttgccc ttgtgcggac cggtggggta cggctcgatc gggatccccc
      481 gaccgttgtc ctccacgcgg caaccgccat cgggttgcag cgtgacgacg atgcgatcgc
      541 agtatcccgc catcgcttcg tccaccgagt tgtcgaccac ctcccacacg agatggtgca
      601 ggccggcaag accggtggac ccgatgtaca tgcccgggcg cttgcgcacc ggctccaggc
      661 cctcgaggac ctggatggcg gacacgtcat agtcgccgcc tgcgctcttt tcactcacgt
      721 ccttcgccaa ggcgcgacct cttcacgctg ctcgatcacc ggcggaacag gccgctcccc
      781 cgccgtccgg tggaggtaac cagcccttct cacgatgtct gtcagtctac caacggggtg
      841 tgtcggagag gtgtgacgcc gagccttgcg accccccgcc aaggacccga atctcggccg
      901 agggtgcgga gcgtgcgtcg ccgctcgcac gtcagcaacg ccgtacttct cgccctctag
      961 cgccgccgta ctcggacctc gatccgcacc actccgtcca cgccggcgac ggtccggaga
     1021 cgctcgagga cgtcatgctc gaggtagcgc aactccgtgg cccaggtcgg gtggtccacg
     1081 tcgacgatca ggcagccgtc caccagcgaa gcgggctgcg catgagcggc gatctgcggg
     1141 ccgaccgcgt cgtcccaact cgcgaagacg cccgccaacg tgcgtgcgct ggtgccccgc
     1201 agcgatcgga cgagttggtc gacgctctcc tggagcgggc ggggctcgtc tgatgcggtc
     1261 atgcgcacac cgttccgcgc tccactcgga ggatgcgttc gacatcggct ccgggcggca
     1321 acggaccggc cgtggtcagc acgatctggc cgaccgggag atgcgtgagg agcgccgctc
     1381 cacgctgttc gtcgagctct gagaagacat cgtcgagcag caacaccggc gtcgttcgcg
     1441 tgcgctcggc gatcagtcgg tgggcaccga gacgaagcgc cagcgccagc gtccgctgct
     1501 caccttgcga ggcgtgcgtg cgggccggca ggccacccac cgtcagttgc agctcgtcac
     1561 ggtgcggacc gacgaggcac acctgccggc gcacttcgtc ggcccgagcg actcggagcg
     1621 cttcggccag accggccgcg cgccatgccg gttcgtagcc gagggtgacg tcgctcgggc
     1681 gcgacgccag gtcgtcgtac gctcgagcca cttcgggggc gagggcgtcg accaactcgc
     1741 cgcgggcctg gccgatcgcg tcaccggctt cggccagcct ggcgtcccag acgtcgagcg
     1801 tgaaggtggt gtcctcaccg agacgaccgc ccgactgctt gagcaacgcg gccttttggc
     1861 gcagcacccg ctcgacctca cggcggagac ggtcatgacg tggatgcagg gcgaccagcg
     1921 tgtcatcgag gaaccgacgc cgaacaccgg gcgcgtcctt cacgagcgcg aggtcatccg
     1981 gggagaagac gctcacgcgc agcacaccca acaggtcgga cgtgcgctgc aagcgctgct
     2041 tgttcacctg gacccgggat cggccggtca gcccgagctc gcactcgacc gcaacgagcc
     2101 tcccgtcatc ctgctcgacc tcgccgcgca ccaccgcccg ctcgcagccg tcacgtacga
     2161 gcgcggcggg cgcggcgccc cggaagctct cgagcgttgc cagataggcg acggcttcca
     2221 ccagattggt cttcccttca ccgttggcac cggtgacggc ggtgaaacct cgggcgagct
     2281 cgacttcggc tacttcgtag ttgcggaagt caacgagctc gagccgccgg acgagcacgc
     2341 gtcacggcac ccgcacgggc atcagcaggt agaggtagtc ctcgcgatcc accgggcgca
     2401 cgaccgccgg cttgagcgcg tcgagggtgt cgagcacgac ctccgcaccg gccgcggcct
     2461 cgacgccgtc gaagaggtac tgcgggttga aagcgacggt gaggggcgtg ccttcgaacc
     2521 gcgcttcgac caccgcgtcc gctgtcccct ggtcctggtt caccgtgttc agccggacct
     2581 cgtcggtctc gatgttcaac cggaccggcg tggcatcggt ggccatcacc cgaacccgct
     2641 tgatcgcttc gagcaacggg tcacgggcga tgaccaggcg atttgggtag gacgacggaa
     2701 tcagctggcg gtagttcggg aactcacctt cgatcagccg ggtcgtcagg cgggtgccat
     2761 cgagctcgaa ggtggcatcg cgctcggcca ggcgcagggt gacatcgccg gagttgggca
     2821 acacccgttc gagctcccgt agcgcacggg agggcagcag gaccttctgg cccgacgcca
     2881 ggacggagac ctcgggcagg tcgcacaccg ccaaccgata ggagtcggtg gcgacgagcc
     2941 ggagtccgcc ttcttcggca gccagcagca cgccggtgag gatcggccga gattcatcct
     3001 tgctcgctgc tcgctcgacc tgactgaccg cctgaccgaa cgaggacgca ggcaacgtca
     3061 cgctggcacc agacgggatg ctcagccgag gaaagtcgtc gaccgcgaac gggcggacgg
     3121 agaacgaact gcggcccgac gtgatgtcga cgtgatcgct cgacgcgtcg acggtgaccg
     3181 cacccgactc gagcgtgcgc acagcttccg cggtcagccg gccgggaagc acgctcgaac
     3241 cgtcccgttc cccgctcacc tgcacggtgg tctggatcgt cagctcgagg tcggtgccgc
     3301 tgatctccaa ctcgtcaccg gtgagcacca ggcggacacc cgccagcacc ggttgcgagc
     3361 cggctcggcc ttgcgcggcc cgggccgcgg tgctgagggc ctcacccaag acgtcacgtt
     3421 cgcaacggaa cttcacaggg ccacttcctt cacaactgct gtcatcatca cgtcgttctt
     3481 ccgaacctag tagtggtaat aggtcttgtg gattgtggac gagcgggcat cgatcctggt
     3541 tgctccggac atcacgatcc ccagg
//
LOCUS       DATBFZ010000222         5342 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_2767938, whole genome shotgun sequence.
ACCESSION   DATBFZ010000222 DATBFZ010000000
VERSION     DATBFZ010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 5342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5342
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2767938"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1100..2086)
                     /locus_tag="VH419_06750"
     CDS             complement(1100..2086)
                     /locus_tag="VH419_06750"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020387695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY7043349.1"
                     /translation="MKIISLGGLKVSRIGLGAMTMAGTYTSEGALDDSESIRTIQRAF
                     DLGVTHVDTAEIYGPFHSEEIVGQAVKGRRDQVVIATKFGLVSHGADGPKIRNLDSTV
                     TSIRAAVEGSLKRLGTDYIDLYYQHRVDPNTPIEETANAVAELVSEGKVLHFGLSEAS
                     VDTIRRAHAVHPVSALQTEYSLWTRDPEAEILPLLRELGIGFVPYSPLGHGLLTGQIR
                     SVADFADDDWRKTNPRFTGDNFVRNLAIVDEVRAIGSEIGATPAQTAIAWLLTRGDDI
                     APIPGTRRVARVEENTAADSIELSSDQIERLDNLTPAAGERHDEANMASIDQ"
     gene            2212..3126
                     /locus_tag="VH419_06755"
     CDS             2212..3126
                     /locus_tag="VH419_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013861847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY7043350.1"
                     /translation="MASDQAPDVRGDVRAQIREFLTTRRARVSPQQAGLPVFDTGRRR
                     VPGLRREEVAMLAGISAEYYVRLERGDAAGISESVVDGIVHALQLDEAERGHLTDLLR
                     AASNSRVPRRRSRVHLVRPTVQRLIDSMVGVPAFVLNGRRDLLAANELGRALYAPIYG
                     ELTDPPNTARFTFLNPRASEFFPDWNTVANDTVAGLRTEAGRDPYDRALSDLIGELST
                     RSEDFRVRWANHNVKSHITGRKLIHHPTVGDLDLPFEFIPLPGDPDQNLLTYTAEPGS
                     PAQEALDLLASWTTTTHGHKITSTVDES"
     gene            3239..3547
                     /locus_tag="VH419_06760"
     CDS             3239..3547
                     /locus_tag="VH419_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043351.1"
                     /translation="MFAPARKYILELLKHVLANDISPGLAIGLTPEFDAVSEAYAAMD
                     EQKAIMSPLRVSQRDLWKRCPPSSTLLDPPDQSTLLTGVSWLTSVSLSPCMAGAARSH
                     "
     gene            3565..4710
                     /locus_tag="VH419_06765"
     CDS             3565..4710
                     /locus_tag="VH419_06765"
                     /inference="COORDINATES: protein motif:HMM:NF012506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HEY7043352.1"
                     /translation="MSDDPDRWLSTGDASAKLGVRPQTLYAYVSRGLLTPRRDGRRSY
                     FKIDELEKLEPKSKRTVRRGRIEVAIDSAVTLLESEGRLFYRGEDVQVIAPTWSFERA
                     AELLWSGYDSGEPKPWPVATDTDVPGRTLTDRVRNAMASLAVNAPEIDSQPQVARTIG
                     RRVLPQLVASLPLLGKRETAARAPLARQLWVRLTELPPTADRIAALNCALIVFADHQL
                     VRSTIAARFAASSQAPPLDAVLAAMATHSGVARHGFRTSMEEALRVGGEPPGAYDQVA
                     YSNRDPRADALAPLVRPAASEAGWQTVALALADERPPSADLALASLTVACEMAPTAAE
                     AIYTIARVAGLLAHVAEEYDHPSAFRPSVGYRGPVPSEYLGDDAGPF"
     gene            5074..>5342
                     /locus_tag="VH419_06770"
     CDS             5074..>5342
                     /locus_tag="VH419_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043353.1"
                     /translation="MKVTLARATTGAALLALCLPFAACSSSDSDAKDGTDTTTASAAA
                     DTGICTDKKTVGYVDIYNASPIEKVMSDAAKQVTDTLGWDLKLIDG"
BASE COUNT          960 a         1876 c         1649 g          857 t
ORIGIN      
        1 gccgggctag ccgaagcggc gtccagctgc gcccgggtcg tcgtccaccc aagttgaacc
       61 gccctggaaa tccccggagg ggaactccga ggcacttcac cccgaaccgc ctcgctcgtc
      121 ccgacaccca tccagcagcc agacgcgcct tccagccgta gaccctcaac ctacggaacc
      181 ggctcgatca tccacagctc cgccacgagg agcggcagtg atctcgaggc aacgggcaaa
      241 gtcgaaaccg cccttgatgg tcaagggcga ccccgaccgg tgctggaaca ccggaccgag
      301 gccccggcac aagtgcacag gaggcacgag caccgtgaac ccatcatctc cccacaaccc
      361 caaaccgatc cggaccgcct cggtcgccct cgacatcacc tgccccacct ggtgcgagat
      421 cagccccgag gaacacgcgt cccgcctgtg ggagaacgag ggccgctgcg tccaccagac
      481 ctccacggcc gtcgtcgacc cggtcggcaa acgtatgtgg agcagccacc tcacttctgc
      541 cggccgatcg agctcacgct acgcacgacg accaaccctg ccgggcgaga ggtcatcacc
      601 aagggggccc gacggccgag ccgaggcatg cccacgcact gcattgccgc gccagccgcg
      661 cccgatcggt caccgctcac cacgaggacg agcgcgaagg ccactccggt ccaggcccac
      721 cccagcgcga aggcgatcaa aagacccgcg accgagacgg cggctgggtc actcgtcaag
      781 caaaagcccc ctgacctgct gttcttactt tggtagcggg gcaggattca acatgtgacc
      841 tctgggttgg atcggtgcct tgccgaccgc taccgaacgt tgcgggatgc agccggatcc
      901 tccgtgttga tgaggagaag tcctcgctga cagttccgac tctatccgag ggactcctga
      961 cgattccgac tgttcttgga gggtaagcgg agggcgatca agtcagtcgc gatctgggcg
     1021 cggacaggag gcccgctgac atcgagcagc ggttgagtcc ctggttgacc gaaacggcag
     1081 cagggcccca ccaggtcggt cactgatcga ttgatgccat gttcgcctcg tcgtggcgtt
     1141 cgccggcggc gggtgtgagg ttgtccagcc gctcgatctg atcggagctg agctcgatgc
     1201 tgtctgcggc ggtgttctcc tccacgcggg cgactcggcg ggtgccgggg atgggtgcga
     1261 tgtcgtcgcc gcgagtcagc agccaggcga tcgccgtctg cgccggggtg gcgccgattt
     1321 ccgaaccgat agcgcggacc tcgtcgacga ttgccaggtt gcggacgaag ttgtcaccgg
     1381 tgaatcgcgg gttcgtcttg cgccagtcgt cgtcggcgaa gtcggctacc gagcggatct
     1441 gtccggtgag gagaccgtgg ccgagcggcg agtacgggac gaacccgatg ccgagttcgc
     1501 gaagcagggg caggatctct gcttcggggt cgcgggtcca cagtgagtac tcggtctgca
     1561 aggccgacac tgggtgtact gcgtgggcgc gacggatggt gtcgacgctg gcttcggaaa
     1621 gtccgaagtg gagcaccttg ccttcggaga ccagctcggc aacggcgttg gccgtctcct
     1681 cgatgggggt gttcgggtcg acccggtgct ggtagtagag gtcgatgtag tcggtgccca
     1741 gtcgcttcaa ggagccttcg acggcggcac gaatgctggt gacggtgcta tcgagattgc
     1801 ggatcttcgg cccatcggca ccgtgggaga ccagcccgaa cttggtggcg atgaccactt
     1861 ggtcacggcg gcccttgacg gcctggccaa cgatttcctc gctgtggaac gggccgtaga
     1921 tctcggccgt gtcgacgtgg gtgacgccga ggtcgaacgc ccgctggatg gtgcggatgg
     1981 attcggagtc gtcgagagcg ccttcgctgg tgtacgtccc ggccatggtc atcgcaccga
     2041 gcccaatgcg ggagaccttg agacctccaa gggagatgat tttcatgctt tcgtgtcctt
     2101 cgtagttggg ttcctccaga agaccagcgc cgcgaacacc gcagaagacc ctgttgagag
     2161 tggtactcgc agtgactcct tagccgcctg accggctcgt agggtggatc catggcgagt
     2221 gaccaagcac ccgatgtgcg aggtgatgtg cgcgcccaga tccgcgagtt cctcacgacc
     2281 aggcgcgccc gtgtcagccc gcagcaggct gggctccccg tcttcgacac cgggcgtcga
     2341 cgcgtccccg gacttcgtcg cgaagaagtc gcgatgctgg cgggcatctc agccgagtac
     2401 tacgtccgcc tcgaacgtgg cgacgcagcc ggcatctccg agagtgtggt tgacggcatc
     2461 gtgcacgccc tccaactcga cgaggcagaa cgaggccacc tcaccgatct gctccgtgcc
     2521 gcaagcaaca gtcgagtgcc acgtcgacgc tccagagtcc atctcgtgcg tccgaccgtc
     2581 cagcggctca tcgattcgat ggtcggagtc ccggcattcg tcctcaacgg acgccgggat
     2641 ctcctcgcgg ccaacgaact cggccgcgcg ctgtacgcac cgatctacgg cgagttgacc
     2701 gacccgccca acacagcacg cttcaccttc ctcaaccctc gcgccagcga gttctttccc
     2761 gactggaaca cggtcgccaa cgacaccgtg gcagggctac gaaccgaggc cggtcgcgat
     2821 ccgtacgacc gtgccctgag cgacctcatc ggcgagctct ccacccgcag cgaggatttc
     2881 cgcgtccgct gggccaacca caacgtcaag agccacatca ccggtcgcaa gctcatccac
     2941 caccccacag tcggcgacct ggacctgcca ttcgagttca tcccgctgcc cggcgatccc
     3001 gaccagaacc tcctgacata caccgccgaa cccggctctc cagcacaaga ggccctcgac
     3061 ctcctagcca gctggacgac cactactcac ggtcacaaga tcacgagcac cgtcgacgaa
     3121 tcctgacccc acaagactct tgcccctgcg ctgagttcac ccagcccgac gcggttcccc
     3181 ctctacgaaa acttcgacta gaaggcgccg ttctggaagg acgtcggcat ccgcgggggt
     3241 gttcgcgccc gcgcgcaagt acatcctcga actgctcaag cacgtgctcg ccaacgacat
     3301 cagccccggg ctggccatcg gcctgacccc ggagttcgac gctgtctccg aggcgtacgc
     3361 cgccatggac gagcaaaagg cgatcatgtc accgcttcgg gtcagccagc gcgacctctg
     3421 gaagcgttgc ccaccgtcgt caacgttgct tgacccacct gatcaatcaa cgttgctgac
     3481 tggcgtatct tggttgacat cggtgtcctt aagcccgtgc atggccggcg ccgcacgtag
     3541 ccactgagag gaaccttgag gcgcatgagc gacgaccctg accgctggct ctctaccggc
     3601 gacgcgagcg ccaagctggg ggtgcggcct cagacgctgt acgcctacgt cagccgcgga
     3661 cttctcacgc cgaggcgcga cggtcggcgc agctacttca agatcgacga actcgagaag
     3721 ctcgagccaa agagcaagcg caccgtccgc cggggacgca tcgaggtcgc catcgacagc
     3781 gccgtgacgc tgctcgagag cgaaggtcgg ctcttctaca ggggcgagga cgtgcaggtg
     3841 atcgcgccca cctggagctt tgagcgtgca gccgagcttc tctggtcggg ctacgactcg
     3901 ggcgagccga agccgtggcc cgtcgccacc gacaccgacg tgccaggccg caccctgacc
     3961 gacagagttc gcaacgcaat ggcctcacta gcggtgaacg ctcccgagat tgacagtcag
     4021 ccgcaggttg ctaggaccat cggccggcgg gtcctgcccc agctcgtcgc ctcgctgccc
     4081 ctcctcggca agcgtgagac tgccgcccgc gcgccgctgg cccgccagtt gtgggtgcgc
     4141 ctcaccgagc tcccgccgac cgcggaccgc attgccgccc tgaactgcgc gctgatcgtt
     4201 ttcgctgacc accagctggt gcgctccacc atcgcagccc ggttcgccgc ctcctcacaa
     4261 gccccgcccc tcgacgccgt ccttgcagcg atggccaccc actcgggagt ggcccgccac
     4321 ggtttccgga cctcgatgga agaagcgctc agggtcggcg gtgagccccc cggcgcctac
     4381 gaccaggtcg cctacagcaa ccgcgacccg cgagccgacg cgctcgcccc gcttgtacga
     4441 ccggccgcct ccgaagctgg ctggcagacg gtggcgctgg ccctcgccga cgaacggcct
     4501 ccgagcgctg accttgccct cgcgtcactg actgtcgcgt gtgagatggc gcctaccgcg
     4561 gcggaggcga tctacaccat cgctcgtgta gccgggctcc tggcccacgt cgccgaggag
     4621 tacgaccacc cctccgcgtt ccgtccgagc gttggctacc gcgggccggt gccgtcggag
     4681 tacctcgggg acgacgcggg ccccttctag gccgcctacc ggtctacgcc gcctcacctc
     4741 acaccggatg tcagggatgg gtcggcgacg tttccgcgcg cccgcgtcaa cgtaacttca
     4801 cgatcagaac atgttgactc ttgtgcaatc aacattgacg atgtagtgtc cgtcacacga
     4861 cggaggggac gacgtacacc cccctctcga aacgagcggg tccggtgcta aggccagccc
     4921 cgcaaagcag gcaaccggcc gcggctccac gacctggatc cacccagccg tctgcttgcg
     4981 gcccattgga gcccgcgccc gcgggactcc agcacatgtc agccccacgg gcgggcggac
     5041 gacagcatca gcagacaaca aggagctcaa cccatgaagg tcactctcgc cagggcaacg
     5101 acgggagcag cactgctcgc cctttgcctg ccattcgccg cctgctcttc gtcggactct
     5161 gacgccaagg acggcaccga cacgacgact gcttccgcag ccgccgacac gggcatctgc
     5221 accgacaaga agacggtcgg ctacgtcgac atctacaacg cctcacccat cgagaaggtc
     5281 atgtcggacg ccgcgaagca ggtcaccgac accctcggct gggacctgaa gctcatcgac
     5341 gg
//
LOCUS       DATBFZ010000223         3532 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_3683373, whole genome shotgun sequence.
ACCESSION   DATBFZ010000223 DATBFZ010000000
VERSION     DATBFZ010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 3532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3532
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3683373"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..1221)
                     /locus_tag="VH419_06775"
     CDS             complement(52..1221)
                     /locus_tag="VH419_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019046270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEY7043354.1"
                     /translation="MTGFETLGLVVAVLAVVAIVLTWTARRRDDFGTTADRATFRTLH
                     TASLASPPLREGLTTTSAEKSLRYLRTLLGAPAAALTDTDAVLGWDGGGEHHRASAYD
                     VGGETLRTGATSAAQVTCEHYDCSLRHVVTAPLTVGERVVGTLQVYVPTPSAGLVRAT
                     NEVAGWVSTQLELAEVDESRARIMEAEVRALRAQISPHFIYNSLSAIASFVRTDPERA
                     RELLLEFADFTRYSFRRHGEFSTLAEELRSIERYLVLEKARFGDRLSVTLRVAPEALP
                     VTLPFLCLQPLVENAVRHGLEGKAEAGHITIDAEDAGSEVVIAIEDDGVGEDPERVRR
                     ALAGDSTVDSVGLGNVDERLRSTFGDDYGLVIETGVGAGTRVVVRVPKYAPGVHT"
     gene            complement(1248..2066)
                     /locus_tag="VH419_06780"
     CDS             complement(1248..2066)
                     /locus_tag="VH419_06780"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY7043355.1"
                     /translation="MDAGEKAIRAASFGAVAATYDEFRPGYPAAAVEWMVGTSPGTLL
                     ELGAGTGKLTKSLATIGHQVVATDPSADMLRRLAGAAPTAVRVIGRAEAIPLPSSSVD
                     AVVAAQAFHWFDHDRALPEIVRVLRPGGVLALVWNSADQKVPWVKKVFGLVGLGDADV
                     GKDPVEDSELFAPSERLVVRHWQEIDRDSLIGFCASQSHVLAQSDADRRAVLDQVGAI
                     YDSYGRAADGMQLPWNTYCYRARVSGLSAPMHSYPEHHAPDEDDPDDGLLVEFR"
     gene            2132..2854
                     /locus_tag="VH419_06785"
     CDS             2132..2854
                     /locus_tag="VH419_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017933275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEY7043356.1"
                     /translation="MLDVEEVESWAALEGVPSAMAAARDAVDVLLADRGLRRTTPELT
                     ADSLLRGAAASATLEGSSTRLEQLRTGDADPVAMAAARVNAGLLALVPVVQRSPLQVL
                     ARLHTLAATGLTDPASLGRPRSEAGVASGLQALSALLLARTSAPAIAVAGVAHAEIMS
                     LAPFGVANGLVARALERLLLVARGVDPTSMLVPEAGHLTLEPSYRAALVAYADKTPAG
                     RRTWLLHVAAAVSGGVEASPVR"
     gene            complement(3147..>3532)
                     /locus_tag="VH419_06790"
     CDS             complement(3147..>3532)
                     /locus_tag="VH419_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020392791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HEY7043357.1"
                     /translation="TRMVQQDGRYTGEIAFYAYGPNKATAIQELAEAEGYDLDASFAY
                     SDSSTDLPMLELVGHPFAVNPDRALRKTAIERGWPVLDFVKPVQLRKRVPFEARKGTL
                     AAVAVGAGVVAAGAVLLSAKRSRRT"
BASE COUNT          537 a         1199 c         1256 g          540 t
ORIGIN      
        1 tttacacgcc ttttgggctg taaacatcgc aaaccccgga ggggaaccgg gtcaggtgtg
       61 gacgccgggg gcgtacttgg ggacgcggac caccactcga gtcccggcgc cgaccccggt
      121 ctcgatcacc aggccgtagt cgtcgccgaa ggtcgaccga agccgctcgt cgacgttccc
      181 gaggccgacc gagtcgaccg tggagtcgcc ggcgagcgcc cggcgtaccc gctccggatc
      241 ctccccgaca ccgtcgtcct cgatggcgat cacgacctca gaaccggcgt cctcggcgtc
      301 gatggtgatg tgccccgcct cggccttgcc ttcgaggccg tggcgcacgg cgttctcgac
      361 cagcggttgc aggcagagga acggcagtgt caccggcaat gcttcgggag ccacccgaag
      421 ggtcacactc agccggtcgc cgaagcgggc cttctccagc accaggtacc gctcgatcga
      481 cctcagctcc tcggccagcg tgctgaactc gccgtggcga cggaacgaat agcgagtgaa
      541 gtcggcgaac tcgagcagca gctcgcgcgc gcgctcagga tcggtgcgga cgaaggacgc
      601 gatcgccgac agcgagttgt agatgaaatg cggcgagatc tgagcacgca gcgcgcggac
      661 ctccgcctcc atgattcgcg cgcgagactc gtcgacctcc gcgagctcga gctgagtcga
      721 gacccatccc gcaacctcgt tggtggcccg gacgagcccc gcgctcggag tgggcacgta
      781 gacctgaagc gtgccgacca cgcgttcgcc gacggtcaac ggcgcggtga cgacgtgacg
      841 cagtgagcag tcgtagtgct cgcacgtcac ctgtgctgcc gacgtggcgc cggtccggag
      901 cgtctcgccg ccgacgtcgt acgccgatgc tcggtgatgt tcaccgccgc cgtcccaacc
      961 gagcacggcg tcagtgtcgg tcagggcggc agcgggggcg ccgagcaacg tgcggaggta
     1021 gcgcaacgac ttctcggccg acgtcgtcgt gagcccttcg cgcagtggtg gcgacgccag
     1081 gctcgctgtg tgaagcgtcc ggaaggtcgc ccggtccgcc gtcgtaccga agtcgtcgcg
     1141 tctgcgggcg gtccaggtca gcacgatcgc aaccacggcc aggacggcca ccacgaggcc
     1201 cagcgtctcg aatccggtca tcggcgctgt ccgaggcagc ccggcgctca gcggaactcg
     1261 acgaggagtc cgtcgtcagg gtcgtcctcg tcgggagcat ggtgttcggg gtacgagtgc
     1321 atcggagccg agaggccgct gacgcgggcg cggtaacagt aggtgttcca cggcagctgc
     1381 atgccatcgg cggctcgccc gtacgagtcg tagatggcgc ccacctggtc gagcactgcg
     1441 cgccggtcgg cgtcggactg cgcgaggaca tgtgactgcg acgcgcagaa gccgatcagc
     1501 gagtcgcggt cgatctcctg ccagtgtcgg accaccagcc gctcgctcgg cgcgaacagc
     1561 tcggagtcct ccaccgggtc cttcccgaca tcggcgtcgc cgaggccgac caggccgaag
     1621 accttcttga cccaggggac cttctggtcc gcggagttcc acaccagtgc gagtacgccg
     1681 ccggggcgca agacgcggac gatctcgggc agcgcccggt cgtggtcgaa ccagtggaac
     1741 gcctgcgccg ccacgaccgc gtcgacggag ctgctcggca acgggatcgc ctcggctcga
     1801 ccgatcaccc gcaccgccgt cggagcagca ccggcgagcc gacgcagcat gtccgccgat
     1861 ggatccgtgg cgaccacctg gtggccgatg gttgccaggc tcttcgtgag ctttcccgtc
     1921 ccggcaccga gctcgagcag ggtgcccggg ctcgttccga ccatccactc gacggccgcg
     1981 gcaggataac ccggccggaa ctcgtcgtag gtcgcagcga cagcgccgaa gctggcggcc
     2041 cgtatggcct tctccccggc gtccatgctc gcgacattag cccaccactc gcgtcgcgag
     2101 ggcagcgcgg aggccgcctt aggctgtctc ggtgctggac gtcgaggagg tcgagtcctg
     2161 ggctgccctg gagggcgtgc cctcggcgat ggcggccgct cgagacgccg tggacgtgct
     2221 gctggcagac cgtgggctgc ggcgcacgac tccggaactg acggcggact cactgcttcg
     2281 gggtgcggcg gcgagcgcga ccctggaagg cagcagcacg cggctggagc agctgaggac
     2341 gggggacgcg gacccggtgg cgatggcggc agcccgagtc aacgccggcc tgttggcgtt
     2401 ggtgcctgtc gtgcagcgct cgccgctcca agtgctcgct cgacttcata cgttggccgc
     2461 gacgggactg acggacccgg cttcgcttgg ccgcccgcga tcggaagcag gagtcgcgtc
     2521 tggactgcag gcgttgtctg ccctgttgtt ggcacggact tccgcccctg cgatcgctgt
     2581 ggcgggagtc gcgcacgccg agatcatgtc gctggcgccg ttcggggtgg ccaacggcct
     2641 ggtggcacgg gcgctggagc ggctgctgct ggtcgcccgg ggggtcgacc cgacctcgat
     2701 gttggtcccg gaggcgggtc atctgacgct cgagccgtcg taccgggccg cgttggtggc
     2761 ctacgccgac aagacacccg ccggcagacg tacctggttg ctgcatgtcg ccgcggccgt
     2821 gtccggcggg gtggaggcga gtccggtccg gtgacgggcg gcgtggtgtc cggtacgtca
     2881 cgaggtggca gtcgcggagc aggtcgacac gccgatagcc cttcccctca ggacgtgaac
     2941 ggcgtacaaa ggaagcacaa ctccagaccc gcacatgagc ccggtaccca cgcggtgacc
     3001 cacccttcgt agagggcagt cgctcccggg acactcccga ggcgacgttt gaggacgtgc
     3061 acgcctggta gccggaatct catgtgtcag cggcggcgtc ttccttccga gggcgccgct
     3121 cgcatgtgcc tggacgtgcc gtcgggtcag gtacgccgcg aacgcttggc cgagagcagg
     3181 acggcgccag cggcgacgac gccggccccg actgcgactg ccgccaaggt gcccttgcgt
     3241 gcctcgaacg gcacccgctt gcggagctgg accggcttga cgaagtcgag caccggccag
     3301 ccccgctcga tcgccgtctt gcgcagtgcc ctgtcggggt tcaccgcaaa gggatgaccg
     3361 accagctcga gcatcggcag gtcagtggac gagtcggagt aggcgaagga cgcgtcgagg
     3421 tcgtacccct ccgcctcggc gagctcctgg atcgcggtgg ccttgttggg gccgtatgca
     3481 tagaaggcga tctcgccggt gtagcggccg tcctgctgca ccatccgcgt gg
//
LOCUS       DATBFZ010000224         2579 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_3683560, whole genome shotgun sequence.
ACCESSION   DATBFZ010000224 DATBFZ010000000
VERSION     DATBFZ010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2579
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3683560"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..869)
                     /locus_tag="VH419_06795"
     CDS             complement(66..869)
                     /locus_tag="VH419_06795"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017933273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEY7043358.1"
                     /translation="MSRISGRSAAFFDLDKTIIAKSSTLAFSRSFYQGGLINRRSVLR
                     SGYAQFVFLLGGADHDQMERMRTYLSEQATGWHVQTVKDIVADALHHIVDPLVYDEAV
                     SLIEQHHLEDRDVVIVSTSGAEVVEPIGDMLGADRVIATRMVQQDGRYTGEIAFYAYG
                     PNKATAIQELAEAEGYDLGASFAYSDSSTDLPMLELVGHPFAVNPDRALRKTAIERGW
                     PVLDFVKPVQLRKRVPFEARKGTLAAVAVGAGVVAAGAVLLSAKRSRRT"
     gene            1216..2319
                     /gene="ssd"
                     /locus_tag="VH419_06800"
     CDS             1216..2319
                     /gene="ssd"
                     /locus_tag="VH419_06800"
                     /inference="COORDINATES: protein motif:HMM:TIGR03815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein Ssd"
                     /protein_id="HEY7043359.1"
                     /translation="MCPSAIRLPNRPSADGVDSPCALVVTHDAELAADVLRLCAAAGV
                     TPDLAERTDVVRGQWRTATCVLLGSDVVDTVARLSLPRRGDVVVVTRGEESTALLRHA
                     VHVGAMRVVRVSDGQDWLVAWLADTAEGVGGAEGIAVIGARGGAGASTIATTLALRAG
                     RRARTCLIDADPLSGGLELLLGSEHREGLRWPDVAVTRGRIGAAAFRAALPSHRGVTV
                     LSWAPGTTASLDAATMRCVLAAAARSSDVVVVDLPRRLDEAADEGLAACRHIVLVTTD
                     DVRSVASASTVLARLGARAADVELVVRTVGAGGPAPEVIADSLGLQLAGVVPTRRCVA
                     RSIDDGLGPPGRGRLVSRCDTVLARLLTADATS"
     gene            2316..>2579
                     /locus_tag="VH419_06805"
     CDS             2316..>2579
                     /locus_tag="VH419_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020577098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CpaF"
                     /protein_id="HEY7043360.1"
                     /translation="MNGIEPDVLDRVRARLAEAGSACTPLDVAAALRSEGALVGDAAV
                     LDVVDALRRESVGAGLLEPLLARDAVTDVLVNGPDEVYVDAGAG"
BASE COUNT          343 a          868 c          943 g          425 t
ORIGIN      
        1 gcggcgtctt ccttccgagg gcgccgctcg catgtgcctg ggctgtgcct ggggcgggcc
       61 ccggatcagg tacgccgcga acgcttggcc gagagcagga cggcgccagc ggcgacgacg
      121 ccggctccca cagcgaccgc cgccaaagtt cccttgcggg cctcgaacgg cacccgcttg
      181 cggagctgga ccggcttgac gaagtcgagc accggccaac cacgctcgat cgccgtcttc
      241 cgcagcgccc tgtcggggtt caccgcaaag ggatgaccga ccagctcgag catcggcagg
      301 tcagtggacg agtcggagta ggcgaacgac gcgccgaggt cgtacccctc cgcctcggcg
      361 agctcctgga tcgcggtggc cttgttgggg ccgtatgcat agaaggcgat ctcgccggtg
      421 tagcggccgt cctgctgcac catccgcgtg gcgatcacgc ggtcggcgcc gagcatgtcg
      481 ccgatgggct cgaccacctc cgcgccggat gtcgacacga tgacgacgtc gcggtcctcg
      541 aggtggtgct gctcgatgag gctgacggcc tcgtcgtaga cgagcggatc gacgatgtgg
      601 tgcagggcgt cggcgacgat gtccttgacg gtctggacat gccagccggt ggcctgctcg
      661 gacaggtacg tgcgcatccg ctccatctgg tcgtggtcgg cgccgccgag caggaagacg
      721 aactgtgcgt atccgctgcg gagcaccgaa cgacggttga tcagaccgcc ttggtagaac
      781 gaccggctga acgcgagcgt gctcgacttc gcgatgatcg tcttgtcgag gtcgaagaac
      841 gcggcagatc ggcccgagat gcgtgacatc gagccgtacc tccgtaattg cgtgagatgc
      901 ccccggctca tcgcatgacg accggtgagt cccccttggc ttcgagcata cggtctgacg
      961 tgccggtttg cgcgaaggcc gcagcggggg gatcatggag gccgagagcc agcggcatcc
     1021 cccctcgtcg ctggacgtga tgggtcgatt ccatcccccg gaatcgagac ccgaggcggc
     1081 ccccggtacc cccccaccgg gggtcgtcgc atgtctcgac ggcgcgcgtc cacagtcagc
     1141 gggtcgcacg gcattcgtcc acacgtcgcc gcggtcgcct gtccggccag gttcgtgcgc
     1201 cgcagcctga tggccatgtg cccttccgcc atccggcttc cgaaccgacc gtccgccgac
     1261 ggagtcgatt cgccctgcgc gctggtcgtc acccacgacg cagagctggc ggccgacgtc
     1321 cttcggctct gtgcagcggc gggcgtgact cccgacctcg ccgaacgcac ggacgtcgtc
     1381 cgcggtcaat ggcggactgc gacgtgtgtc ctgctcggca gtgacgtcgt cgacaccgtc
     1441 gcacggctgt cactgccgcg ccgtggcgac gtggtcgtgg tgacgcgtgg tgaggagtcg
     1501 acggcgctgc tgcggcacgc tgtccatgtc ggtgcgatgc gggtggtccg cgtcagcgac
     1561 gggcaggact ggctcgtggc ttggctcgcg gacaccgccg agggtgtggg cggcgccgag
     1621 ggcatcgccg tgatcggtgc gcgtggcggt gccggcgcgt ccacgatcgc gaccacgctg
     1681 gccctgcgcg ccggtcgcag ggctcggaca tgcctgatcg acgccgaccc gttgtccggt
     1741 ggcctcgagc tgctcctcgg cagcgagcat cgcgagggtc tgcgctggcc cgacgtcgct
     1801 gtcacacgcg ggcgcatcgg cgccgcggcg ttccgtgcgg cgctcccttc ccatcgcggc
     1861 gtcacagtgc tgtcgtgggc tcccggaacg acggcgtcgc tggacgcggc gaccatgcgc
     1921 tgcgtgctgg cggcggcagc gcgcagctcc gacgtcgtgg tcgtcgatct gcctcgtcgg
     1981 ctcgacgagg ccgccgacga aggcctggct gcatgtcgtc acatcgtgct cgtcaccacc
     2041 gatgacgtcc ggtccgtcgc gtccgcatcg acggtgctgg cacggctggg agcccgtgcg
     2101 gcagacgtcg agctggtcgt gcgaaccgtt ggtgcgggtg gtccggcgcc ggaggtgatc
     2161 gccgacagtc tcggtctgca gctcgccggt gtcgtgccga cccgccgttg cgtcgcgcgc
     2221 agcatcgacg acgggctggg tccgcccggc cggggacggc tggtgagtcg ctgcgacaca
     2281 gtcctggcac ggctgctcac ggcggacgcg acgtcgtgaa cggaatcgag ccggatgttc
     2341 tcgaccgtgt gcgagcccgg ctcgcggaag cgggttcggc ctgcacaccc ctcgacgtcg
     2401 cggcagcgct gcggtccgag ggtgcgctgg tcggcgatgc ggcggtgctg gatgtggtgg
     2461 acgcactccg ccgcgagagt gtgggcgccg gactgctcga gccgttgctg gcgagagacg
     2521 cagtcaccga cgttctcgtg aacggccctg acgaggtgta cgtcgatgct ggtgccggg
//
LOCUS       DATBFZ010000225         2409 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_3427327, whole genome shotgun sequence.
ACCESSION   DATBFZ010000225 DATBFZ010000000
VERSION     DATBFZ010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2409
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3427327"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1666)
                     /locus_tag="VH419_06810"
     CDS             complement(<1..1666)
                     /locus_tag="VH419_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043361.1"
                     /translation="MADQTVAVRPDSPATPRIEYPDPPPTTAPRVDGELAAYCRQLFY
                     RAREHRRPIIATWNRNDRLLRNRTWLADRAGWLPSPEVPEIRPIISQLAGWMTDQRPT
                     YTAVPAAAPATPYFEVLNKIADDLTTVIDSVATVHRHEENIKQAVWDSYAWGTGLFKA
                     TWDMTLDGGLGNAVLLRVDPYTFYPDPAARNLTDGNYYIEARNMSRQEIDRRFHAGNL
                     FADGSGPTEDIDVHPDRLGNAPRASARTFPGQAAPMGPSTRSPSLSTRFSPGDPDPND
                     RGVTVLECWLRQHEPIDINGRSSLFDYWRCVVVAGNHVLLDVPAYQLWSHGQHPYQRF
                     VADDTGEFWGQSLVEDLASCQITLNRLLAGVIHNIDLCGNPPFLEGGRSGIPRTRIPN
                     RPGTRITVTGDINQAKWMEPVPLHPIIPQLVQFFVTEMERISGLSAITRGFTPTGRNA
                     SDVLDSVQEAGFTRIRSHLRGLEWALRAAYDQIAGLIVENYDTPRLLAITGPQGQPSA
                     MALASRHFHVPMLDRNNQVVQHPLRFTIISQIGAHISQMQERAETIQ"
     gene            complement(1666..1890)
                     /locus_tag="VH419_06815"
     CDS             complement(1666..1890)
                     /locus_tag="VH419_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043362.1"
                     /translation="MDDAERLAALLRSLTNITPTAGQVDRIELLRDKAKMFGQAVIES
                     CPDSRERSLALTHLEAAVMWAVKSIMVEGA"
     gene            complement(1890..2276)
                     /locus_tag="VH419_06820"
     CDS             complement(1890..2276)
                     /locus_tag="VH419_06820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043363.1"
                     /translation="MPAYEYRCDVCALSFTSGWRDDEMICVRCTRMARRVWGFSILPS
                     FPGVPAAHVSDLARASEEASSPQTLYDADGNAHAVERPEHRFVPVDLRDREALGVTSE
                     GLDSTYDQLRRVGNDPAADRLRKVMD"
BASE COUNT          375 a          774 c          847 g          413 t
ORIGIN      
        1 gttggatggt ctcggcccgc tcctgcatct gggaaatgtg cgcaccgatc tggctgatga
       61 tcgtgaaccg caacggatgc tgcacgacct ggttgttgcg gtcgagcatg ggcacatgga
      121 aatgccgcga cgcgagggcc atcgccgacg gttggccttg ggggccggtg atggcgagga
      181 ggcggggggt gtcgtagttc tcgacgatca gtccggcgat ctggtcgtag gcggcccgca
      241 gggcccactc gagtcctcgg aggtggcttc ggatacgagt gaagcctgcc tcctgtacag
      301 aatccagcac atcagaagca ttacggccag tgggggtaaa gccacgagtg atagcagaca
      361 ggccactgat gcgttccatt tcggtcacga agaactggac gagctgaggg atgatcgggt
      421 ggagggggac gggctccatc catttcgcct ggttgatgtc accggtgacg gtgatgcggg
      481 tgccgggccg gttcgggatc ctcgtgcggg gtatcccgct gcggccgcct tcgaggaacg
      541 gcgggttgcc gcacaggtcg atgttgtgga tcacgcccgc cagcagccgg ttgagcgtga
      601 tctggcacga cgcgaggtct tcgacgaggg attggcccca gaactctccg gtgtcgtcgg
      661 ccacgaaccg ctggtacggg tgctggccgt gggaccacaa ctggtaggcg ggcacgtcca
      721 acagcacgtg gttgccggcg acgacaacgc accgccagta gtcgaacagg ctcgaacggc
      781 cgttgatgtc gatcggttcg tgctgccgta gccagcattc caacacggtg acgccccggt
      841 cgttggggtc ggggtcgccg ggcgagaacc gggtcgaaag tgacggggac cgggtcgacg
      901 gtcccatcgg cgcggcctgc ccggggaacg tccgcgccga cgccctcggg gcgttgccga
      961 gccggtcggg gtgcacgtcg atgtcctcgg tcggccccga cccgtcggcg aacaggttcc
     1021 cggcgtggaa acggcggtcg atctcctggc ggctcatgtt gcgagcctcg atgtagtagt
     1081 tcccgtcggt caggttccgc gccgcagggt cgggatagaa cgtgtacggg tcgacccgca
     1141 gcaggacggc gttgccgagg ccgccgtcca acgtcatgtc ccatgtggct ttgaacaggc
     1201 cggtgcccca cgcgtaggag tcccacacgg cctgtttgat gttctcctcg tggcggtgca
     1261 cggtcgcgac ggagtcgatg acggtggtga ggtcgtcggc gatcttgttg agcacctcga
     1321 agtacggcgt ggccggcgcc gccgccggca ccgcggtgta cgtgggccgc tggtcggtca
     1381 tccatcccgc cagctggctg atgatgggac ggatctcggg cacctcgggc gacgggagcc
     1441 acccggctcg gtcggcgagc caggtgcggt tccgcaggag ccggtcgttg cggttccagg
     1501 tcgcgatgat cggacggcgg tgctccctcg cccggtagaa gagctgccgg cagtaggcgg
     1561 cgagctcgcc gtccacgcgt ggcgcggtgg tgggcggcgg gtcggggtac tcgatgcgcg
     1621 gtgtcgccgg cgaatcggga cggaccgcga cggtctggtc tgccatcacg ccccctcgac
     1681 catgatgctc ttgacggccc acatgacggc agcctcgaga tgcgtgagag cgagggaccg
     1741 ttcccgcgag tcggggcaac tctcgatcac cgcctggccg aacatcttcg ccttgtcgcg
     1801 gagcagctcg atccggtcga cctgccctgc ggtcggtgtg atgttcgtca acgagcgcag
     1861 cagcgccgcc agtcgttccg cgtcgtccat cagtccatga cctttcgcag ccggtcggcg
     1921 gccgggtcgt tccccacacg ccgcaactgg tcgtacgtcg agtccaaccc ctctgacgtc
     1981 acccctaacg cttcgcggtc acgcaggtcg acgggcacga accggtgctc cggccgctcg
     2041 acggcgtgag cgttcccgtc cgcgtcgtac aacgtctgcg gcgacgacgc ctcctccgac
     2101 gctcgagcca ggtccgacac gtgggcggcg ggcacaccgg gaaagctcgg caggatcgag
     2161 aacccccaca cccgccgcgc catgcgcgta cagcgcacgc aaatcatctc gtcgtcccgc
     2221 caccccgacg tgaacgacaa cgcgcagacg tcgcaccggt actcgtacgc aggcaccggg
     2281 cggattattg ctacgacgct ggttcctcgt cccacgcctg ccacgtccgt tccgtggtcg
     2341 caggctccag caccctgttc accggaccgg cgtaggcctc caacagttca gtcgacgagc
     2401 agatacacg
//
LOCUS       DATBFZ010000226         1845 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_5982643, whole genome shotgun sequence.
ACCESSION   DATBFZ010000226 DATBFZ010000000
VERSION     DATBFZ010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1845
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5982643"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            283..861
                     /gene="sigK"
                     /locus_tag="VH419_06825"
     CDS             283..861
                     /gene="sigK"
                     /locus_tag="VH419_06825"
                     /inference="COORDINATES: protein motif:HMM:NF007228.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF RNA polymerase sigma factor SigK"
                     /protein_id="HEY7043364.1"
                     /translation="MRVPAQRRERTELVELMALVAQGDQSAFAALYDATSATVFGIVR
                     RILLDVAQAEEVTQEVYVDAWRYATRFDAKLGSPASWLNTIAHRKAVDRVRSVERSTQ
                     RDQRHFEAESRRYEPDVSEVVVARDDSRRVREAMQRLPEAQRTALELAYFKGCSQREV
                     AELLDVPLGTIKTRIRDAMRRLRDYLGEEAPT"
     gene            858..1712
                     /locus_tag="VH419_06830"
     CDS             858..1712
                     /locus_tag="VH419_06830"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEY7043365.1"
                     /translation="MTDQPNMSDDSNDVHGELHAYAVGALSTEEASAFERHLASCASC
                     EQELAELYEVTAALSGSVAEEPPSSLRTAVLAEIARTPQEPPSSAVAVAEASRLGPPA
                     TPPSREESGHAERGPAESGSNVVPLRRSRATVVSGLVAAAAVVAAVVFGGVALRNSQD
                     ADDAAAQTAELTRILGADDVQTVPGDPQSAGHSGTIVLSRSEGAAVFVSSTFPGLPED
                     EVYEAWTIEGKQNPVSAGTFTPSGSESILSLPQASFAADVMAITVEPEGGSDQPTTDP
                     VVTFTMPS"
BASE COUNT          299 a          641 c          638 g          267 t
ORIGIN      
        1 tccctttccg ttgccgccga ggtgggtacg tcgccgggtc tcgcgcagca cgtcgggcca
       61 ccaccgtccg tccggcaccg atcgtgtccc ctgggccgcc cgcgacaggt ggcgtcagga
      121 gagcccacac gacgccgaac ggccctgatg ccgtcgccgt ggccccccac tgccatgacc
      181 agctcgctgt cgtccaccgt gcgctcgacc caccggcaac ggctcgaccc tgaggccggc
      241 gaccggtcga ggccgcgctc ggctcatggc acagtggtgt ccatgcgagt gccggcacag
      301 cgccgcgaac gcaccgagct cgtcgagctg atggctctcg tggcgcaggg agaccaaagc
      361 gccttcgccg ccctctacga cgcaacatcg gcaaccgtgt tcggcatcgt gcggaggatc
      421 ctccttgatg ttgcgcaggc cgaggaggtc acccaggagg tgtatgtcga cgcctggcgg
      481 tacgccaccc ggttcgacgc caagctcggc tcgcccgcga gctggctcaa cacgatcgcg
      541 catcgcaagg cggtcgaccg ggttcgctcc gtcgagcgca gcacccaacg ggaccagcga
      601 cacttcgagg cggagagcag gagatacgaa ccggacgtct ccgaggtcgt ggtggcccgc
      661 gacgacagcc gtcgggtgcg cgaggcgatg cagcggcttc ccgaggctca gcgaacagcg
      721 ctcgagctcg cctacttcaa aggctgctcg caacgtgagg tcgccgaact gctggatgtc
      781 ccgttgggga cgatcaagac ccgcatccga gacgcgatga ggcgactacg tgactacctg
      841 ggagaggagg caccgacatg actgaccagc cgaacatgag cgacgactcc aacgacgtcc
      901 atggcgagct ccacgcgtac gccgtcggtg ccctctccac ggaggaggcc tcggcgttcg
      961 agcgacacct ggcgagctgt gcttcctgcg agcaggagct ggccgagctg tacgaggtca
     1021 ccgccgccct gtccggcagt gtggccgagg agccgccgtc ctcgttgcgc acggcggtcc
     1081 tggccgagat cgcccggacg cctcaagaac cgccctcgtc ggcagtggca gtcgccgagg
     1141 cgtcccggct gggtcctccc gcgacacctc cgagccggga ggagtccggg cacgctgagc
     1201 gaggacctgc cgagtcgggc tcgaacgtcg taccgctgcg ccgatcccgc gccaccgtgg
     1261 tcagcggact ggtggctgcc gcggcggtcg tcgcggcggt cgtgttcggt ggtgtcgcgc
     1321 tgcggaacag ccaggatgcc gacgacgccg ccgcccagac ggctgagctc acccggatcc
     1381 tcggcgccga tgacgtgcag accgtgccgg gcgatccaca gagcgccggc cattcgggaa
     1441 cgattgtgct gtcgaggagc gaaggcgccg cggtcttcgt gtcgtcgacg ttccccggcc
     1501 tgcccgagga cgaggtctac gaagcctgga ccatcgaggg gaagcagaac ccggtgtctg
     1561 ccggcacgtt cacgccgagt ggctcggagt cgatcctctc gctgccgcag gcgtcgtttg
     1621 cggccgacgt gatggcgatc acggtcgaac ccgagggcgg gtccgaccag cccaccaccg
     1681 acccggtcgt caccttcacg atgccgagct agtgccgcgt caaccaacgt tgctcgtcgt
     1741 gagcggtgtc cgggtggatg agctgcaagg cagaggaggg aggcgatgcg cagcatcgtt
     1801 gaccgacgat aacgccgcag atcgccgccc gggcggcgcg caaca
//
LOCUS       DATBFZ010000227         7325 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_2117824, whole genome shotgun sequence.
ACCESSION   DATBFZ010000227 DATBFZ010000000
VERSION     DATBFZ010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 7325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7325
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2117824"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(761..1810)
                     /locus_tag="VH419_06835"
     CDS             complement(761..1810)
                     /locus_tag="VH419_06835"
                     /inference="COORDINATES: protein motif:HMM:NF013384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HEY7043366.1"
                     /translation="MIELRSDNAAGVAPEILAAVEAANTGSALAYGGDDLTAHLQEVV
                     RAVFEHPTARVFPVTSGTAANSLALSAATPPWGAVLCHPSAHIIAAEGGATSLLGGGV
                     VMQGVDGAHALIDPEGLRVMLDAVRWGDPHESQPSVLSLTLPSDHGTVYQPDQVTKLT
                     AIGREFGLRAHLDGARIANAVAALGSAPADVTWRAGVDVLSLGATKNGALSAEAIVAF
                     DERIADELVYRTKRSGHVTSKMRFQSAQLVAYLTDGLWLRLATNANRRMAELVAQLQS
                     LASHGVRLQQRVDVNMAFVELPAQAIDAVAAAGVLFYRMGPTSVRLVTSWQTTAGDVT
                     EAASRFREAVLAQAR"
     gene            1973..2440
                     /locus_tag="VH419_06840"
     CDS             1973..2440
                     /locus_tag="VH419_06840"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEY7043367.1"
                     /translation="MTDLVDLARTTLAGTRFMVLGTVDPSGRPRVSPVWFSLVDHRDA
                     YWLSSPAAHHSRNIEERPEVSMVVFDSSADPHTGQAVYLEATAARVPDDELEAACAEA
                     FREVADELSFTPETLRDAPFVLYRARVTASEVHVRGRDFGDGSGPDQRAPVDL"
     gene            2617..3072
                     /locus_tag="VH419_06845"
     CDS             2617..3072
                     /locus_tag="VH419_06845"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY7043368.1"
                     /translation="MTDDHPALVELTRMAQESAEAYLAGDLDTYANVLPHADDFTLMP
                     PFGGPTRHGFILNDETRAATRDTFRGGRVEVEVDASYVTDDLAVLAVVERQRGLVGDI
                     AEQDWSLRVTLAFRRDGGQWRLVHRHADALVHTVGWNTLPRLARGDLPD"
     gene            complement(3163..3564)
                     /locus_tag="VH419_06850"
     CDS             complement(3163..3564)
                     /locus_tag="VH419_06850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043369.1"
                     /translation="MDHEAFCTIEGWTERKRATGKRGTHHVNYELPLPDGRILYTRIS
                     HPVDRTDYGASIWSHILRDQLEVTADEFWNCVNSQVLPDRGQTQPIAEAIPVGVIRTL
                     TQEAHIPEAEVRAMTRAEAIARLTHFYTTGK"
     gene            complement(3600..4016)
                     /locus_tag="VH419_06855"
     CDS             complement(3600..4016)
                     /locus_tag="VH419_06855"
                     /inference="COORDINATES: protein motif:HMM:NF024312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043370.1"
                     /translation="MATASVNYGSAREAREHFKDLLDAADEGRPATVTRDERRVAAVD
                     ADRLVYFLTRLHPSGVEAVADGDGWSVFLPGVPVAADGATIEEAVEEMVSALRDYAEA
                     WTERLRLAPNHEDNWGLVQVVTLASDDQLRNWLLAA"
     gene            complement(4326..4696)
                     /gene="ssrA"
                     /locus_tag="VH419_06860"
     tmRNA           complement(4326..4696)
                     /gene="ssrA"
                     /locus_tag="VH419_06860"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(4849..6018)
                     /locus_tag="VH419_06865"
     CDS             complement(4849..6018)
                     /locus_tag="VH419_06865"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY7043371.1"
                     /translation="MSDERSGAPVDAPAELDAVEVLVVDDQPQNIRLLDAVLSPRGYR
                     VVTASSGEEALTVLSQTRPDLVLLDIVMPGVDGYEVCRRIRDNPATAFLPVVMITASG
                     AQERLRALEAGADDFVTKPFDQSELLARVSSLARVKRYHDTVRRQAEELAAWNRDLEK
                     RVREGVEELERIGRLRRFLSPQLAELVVNSGDEEFLASHRREIVVVFCDLRQFTAFAE
                     SSEPEEVMAVLGEFHAALGDLIFRFEGTLERFTGDGLMVFFNDPMPCDDAAARSVRMA
                     VAMRDRVHELAERWSGLGHELGFGIGIAQGYATLGRIGFEGRFDYAAIGSVTNTAARL
                     CAAAEPWQILVTQRVLTSAGDIVVGQPVGDLQLRGLSKPVRAFDVKGLAAARVSS"
     gene            complement(6011..6397)
                     /locus_tag="VH419_06870"
     CDS             complement(6011..6397)
                     /locus_tag="VH419_06870"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY7043372.1"
                     /translation="MSCRRVLVVEDNAKNLKLVRDVLAYAGYDVIEAETGERGVELAV
                     DHIPDLVLMDLQLPGIDGTEALRRLRELPGMEHVPVVAVTAFAMQADRDRAFASGFDG
                     FVEKPLSVRALPEQVERFLMEGASGE"
     gene            complement(6394..>7325)
                     /locus_tag="VH419_06875"
     CDS             complement(6394..>7325)
                     /locus_tag="VH419_06875"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY7043373.1"
                     /translation="SKVEAGRMELDISEVDVRAALEYGVSLVRERATVHGITLTIECA
                     ADVGLVDTDELRFRQVVVNLLSNAVKFTPDGGAVIARAAIEGGYLVVSVADSGIGVEA
                     PDRERIFESFQQGGRGPSSEEGTGLGLTLSRRIVELCGGQLWLDSEVGVGSTFSFSVP
                     LGSRATVAAGVSEPAPDRGRVEVVLIEDDRPSLDLLTVFLASLDIRVTVARDGVDGLA
                     AVRRVMPAAVILDIRLPRMDGWEVLRVLKTEEATAHIPVIVVSVVDERQRGLGMGAAA
                     YLIKPVGREELVEQLRRIQVLPPTLTRTSSEAP"
BASE COUNT         1232 a         2566 c         2344 g         1183 t
ORIGIN      
        1 gtctgtccct cgtactttcg gctgatacgc gacgcccacc ctcccgagca ggccattgtc
       61 agcggagtcc gaagcctcct tcaaacccgg aacttgacac tcgcgcccga accacgacga
      121 cactgggaaa tgacggacga ccgttcacct agctggaacg actcaagcat ctgaccaacg
      181 cgcctggcga gaggccaatg tgatcgtgac cgatcggcga gcccgcactg gggcatcgtc
      241 accgaggaga ctgtccgcga gggacagatc tcccactggc cggccggcca caccggggta
      301 agcgaggagg gcgtggtctt cctcgaggtt ggccccgttg ggtcgatgcg acagttcggc
      361 gaacacgcgc gccagctgtt cggctgagcg aacgttcgat tgaaccaagg ctgatgccgt
      421 agaacggcgg cgcgacgcgc gcaacgcgag ccgtcaagcc gccatccaat gctcgtggcg
      481 cttgcctcgc ggcctcgttc tccgtatgcc gaaccccaac cgagaacgtc gcaggtcaag
      541 agccctgccg gtgctcgggc gtcccataac accagtccgg ttcgaggttc ggcagccgca
      601 ccctcgatgg gtgcgcaggc cgtgcacgaa ctcattcccg tggcccaaag gatcggacca
      661 cgtccacggc tctccgaagg ccgtcagagt tgagtcatac cgccgcgttc cggcttggtc
      721 cgcgcgcccg atgatctgtc gactcgagga gtcgtagccg tcaccgagcc tgggcgagca
      781 ctgcctcccg gaaccggctt gcagcctcgg tgacgtcgcc ggccgtggtc tgccagctgg
      841 tgacgagccg cacgctggtc ggacccatcc ggtagaacag cacgccagcg gcggccactg
      901 cgtcgattgc ctgcgcgggc agttcgacga aggccatgtt gacgtcgacg cgctgctgga
      961 gccgcacacc gtgcgacgcc aacgactgca gctgcgccac gagttctgcc atccgccggt
     1021 tggcgttggt ggccagccgc agccacagcc cgtcggtgag gtatgccacc agttgggccg
     1081 actggaagcg catcttgctg gtgacgtgtc cggaccgctt ggtgcggtac accagctcat
     1141 cagcgatgcg ctcgtcaaag gcgacgatgg cctctgcgct cagtgcgccg ttcttggtcg
     1201 cgccgagcga cagcacgtcg actccggccc gccaggtgac atcggcgggt gcgctgccca
     1261 gcgcggccac ggcgttcgcg atgcgagcac cgtcgaggtg ggcacgcagc ccgaactccc
     1321 ggccgatcgc ggtgagcttc gtgacctggt cgggctggta cacggtgccg tggtcactgg
     1381 gcagggtcaa ggacagtacg ctcggctggc tctcgtgcgg gtctccccac ctgacggcat
     1441 cgagcatcac ccgcagcccc tccgggtcga tcagcgcgtg agcaccgtcg acgccttgca
     1501 tcaccacacc gccaccgagc aacgacgtcg ccccgccctc cgcggcgatg atgtgcgccg
     1561 acgggtgaca cagcacggca ccccacggag gcgtggccgc cgacagcgcg agcgagttcg
     1621 cggcggtccc gctggtcacc gggaagaccc gggcggtcgg atgctcgaag accgcccgca
     1681 ccacctcctg gaggtgggcc gtgaggtcgt ctccgccgta ggccagagcc gagccggtgt
     1741 tcgccgcctc gacggcggcg aggatctccg gcgcgacacc cgcggcgttg tcactgcgca
     1801 actcgatcac gcgcctactc tgcccctccc gaccaccgac cggcgagggg acgcttcggc
     1861 gcgactcgtg cgacgctcat cagagccgcg agtacgggag tgcggatcgt ggctcggggc
     1921 ggcggcccga aggcgggtga ggtccgaacc tccggtcagc caaactggca cgatgactga
     1981 tcttgtcgac ctggcgcgta cgacgctcgc cgggaccagg ttcatggtgc tcggtaccgt
     2041 cgatccgtct ggtcgtccgc gggtgtcgcc ggtgtggttc tcgctggtcg atcaccgcga
     2101 cgcctactgg ctgtcctcac cagccgccca ccactcgcgc aacatcgagg aacgacccga
     2161 ggtgagcatg gtggtcttcg actccagcgc tgacccgcac accggccagg ccgtgtacct
     2221 ggaggcgact gctgcccggg tgcctgacga cgagctggag gctgcttgcg ctgaggcgtt
     2281 ccgggaggtc gcggacgagc tctcctttac gcctgagacg ctgcgagacg cgccgttcgt
     2341 gctgtaccgg gcgcgtgtca cggcttcgga agtccacgtc cgcggccgcg acttcggtga
     2401 cggttccggc ccggaccagc gggcgcccgt cgacctctga gtgatcaggg aacagcggcc
     2461 ccccaccgca tgctgcgtca caacccgacc tccctcgcac gtcagctaca tcgggcgacc
     2521 tcgaccgcgt acgcgcgcac agggttgctg tcggtgacgc ctcccgggcg cgggtgagcg
     2581 gttcgcgggc ggtgatcgcc tggctagcat tcctggatga ccgacgacca ccccgctctg
     2641 gtcgagctca cccgcatggc ccaggagtcg gctgaggctt atctcgccgg ggacctcgac
     2701 acgtacgcaa acgtgctgcc gcatgcggac gacttcactt tgatgccccc gttcggtggg
     2761 ccgacccgac atggtttcat cctgaacgac gaaacgcgcg cggccacccg agacaccttc
     2821 cgcggcggcc gggtggaggt cgaagtcgac gcgtcgtacg tgaccgacga cctcgccgtg
     2881 ctcgcggtgg tggagcgaca gcgcggcctg gtgggcgaca tcgctgagca ggactggtca
     2941 ctccgcgtca cgctcgcgtt ccgacgagac ggcggacagt ggcggctcgt gcaccggcat
     3001 gcggacgcgc tggtccacac cgtcggttgg aacacgctcc cgcggctcgc gcgcggcgac
     3061 ttgccggact gagcacgcga tggatgatcc actcagcgca gaccgcggga ctcctgcggt
     3121 tgcttgaggc cctccgcgac gtccgagccg gcggcggggc agtcacttcc cggttgtgta
     3181 gaagtgcgtc agtcgggcga tggcttccgc tctcgtcatg gcgcgcacct cggcctccgg
     3241 gatgtgggcc tcctgggtca acgtacggat cactcccacc gggatggcct cggcgatcgg
     3301 ctgcgtctgc ccccgatccg gtaagacctg gctgttgaca cagttccaga actcgtcagc
     3361 agtcacttcc agttggtctc gcaggatgtg cgaccagatg cttgccccgt agtcggtccg
     3421 gtcgacgggg tggctgatcc gcgtgtaaag gatgcggcca tccggcagcg gcagttcgta
     3481 gttgacgtga tgggtccccc gcttgccggt ggcgcgcttg cgctcagtcc agccctcgat
     3541 cgtgcagaaa gcctcgtgat ccatccgcgt tgccggaggc cgcttctggg cacgagccgt
     3601 taagcggcta gcagccaatt gcgcagttgg tcgtcggagg ccagtgtgac aacctgcacc
     3661 agtccccagt tgtcctcgtg gtttggggca agtcgcagac gctcagtcca ggcctcggca
     3721 tagtcgcgca gggcggagac catctcttcg acggcctcct cgatggttgc gccatccgcc
     3781 gcaactggca cacccggaag gaagacgctc cagccatcgc cgtctgcgac ggcttcgaca
     3841 ccagagggat ggagacgggt caggaagtac acgagccggt cggcgtcgac tgcggctacc
     3901 cgtcgctcgt cgcgggtaac cgtagccgga cgaccctcgt cggcggcgtc gaggaggtcc
     3961 ttgaagtgct cgcgcgcctc ccgggcactc ccgtagttga cagatgcggt ggccatcggt
     4021 gctctcctgc actcaacatg tgtacacagt gtacacgccc tgagcgttcc ccattcacct
     4081 ctggacgaga ggctacgagc accggaccag gccggtcgcg catcgcgcga cacagctcac
     4141 cctcgaccgc gacacagctc acctcgatga cacgacccac cccgaacaga ccgtcaccgc
     4201 actcaacacg gccagcccga gcaagggggt gcgggtggcg aagcccccgc ctggcgcggc
     4261 gaagcccgcg cacaactgac gagggcggcc cggtcgcgca cgcgcgacac agctcaccct
     4321 cgacctcgtg gagctgccgg gaatcgaacc cgggtcctct gacgccgaac caagtcttct
     4381 ccgagcgcag cctgcgtcac gcttttctcg gccccggcac ttgtgcagac ccattgccga
     4441 cgggctcagt cgctgtagat gtcccgcagc caccccgcga ccgggtgact gcagcagagc
     4501 ctccttggtg aggccggtga ctggaccgga ggcgcttcca gaccgaccct tcgattactg
     4561 ctcaggcagc aagagcgaag tcagtgctct ttgcgttggc acttatgggt ttccagggag
     4621 tcgtttacga gatgaccctg gctcctcggc tcgcttctct tggctccaac gaccaaagtc
     4681 gaaaccaatc agcccctgtt gagttgtgtc agccgtagag gctacacggt tcaaccgacg
     4741 cttcgcagtc aacattcccg ctcaggcagg cagcggagca gccgttccgc atggtccaga
     4801 gtggacaaca tcacagattg tgcggatcag gtacggcgag ttggccggtc atgagctcac
     4861 acgcgcagcg gcgagtccct tgacgtcgaa ggcgcgcacc ggcttgctga gtccccgcag
     4921 ctgcaggtca cccacgggct gaccgacgac gatgtcgcct gccgacgtga ggacgcgctg
     4981 tgtcacgagg atctgccatg gctcggccgc cgcgcacaac cgggcagcgg tgttggtgac
     5041 actgccgatg gccgcgtagt cgaagcgtcc ctcgaagccg atgcggccca gcgtcgcgta
     5101 gccctgcgcg ataccgatgc cgaacccgag ctcatggccg agaccgctcc atcgctcggc
     5161 gagctcgtgg acacggtcgc gcatggccac cgccatccgg acgctcctcg cagcagcgtc
     5221 gtcgcacggc atcggatcgt tgaagaagac catcaggccg tcaccggtga accgctcgag
     5281 cgtgccttcg aatctgaaga tcagatcgcc cagcgcggca tggaactcac ccaggacagc
     5341 catcacctct tccggctcgg acgactcggc gaacgccgtg aactgccgca gatcgcagaa
     5401 gacgaccacg atctcgcgtc gatgactggc caagaactct tcatcgccgg agttcacgac
     5461 cagctctgcg agctgaggcg agaggaaccg tcgcagccgg ccgatgcgct cgagctcctc
     5521 aaccccttcg cggacccgtt tctccaggtc cctgttccag gcagccaact cctctgcttg
     5581 gcgacgcacc gtgtcgtggt agcgcttcac tcgcgcgagc gaggagacgc gagcgagcag
     5641 ttcgctctgg tcgaacggct tggtcacgaa gtcgtctgcc cctgcttcca acgcgcgcag
     5701 ccgctcctga gcgccactcg ccgtgatcat cacgacaggc aggaacgccg tcgccggatt
     5761 gtcacggata cgccggcaca cctcgtagcc gtccacaccc ggcatgacga tgtcgagcaa
     5821 cacgaggtcc ggccgggtct ggctcaggac cgtcaacgcc tcctcgcccg agctggcggt
     5881 cacgactcga tagccccgcg gactgagtac ggcgtccagc agcctgatgt tctgcggctg
     5941 gtcgtcgacg acgaggacct cgaccgcgtc gagctcggcg ggagcatcga ccggggcacc
     6001 gcttcgttca tcactcaccc gaagctccct ccatgaggaa ccgctcgacc tgctcaggaa
     6061 gcgcccgcac actcaacggt ttctcgacga acccgtcgaa cccgcttgcg aaggcacggt
     6121 ctcgatccgc ctgcatggcg aacgcggtga ccgccacgac tgggacgtgc tccatccccg
     6181 gaagctcgcg aagccgtcgc agcgcttcgg tgccgtcgat ccccggcagc tggaggtcca
     6241 tgaggacgag atccggaatg tgatcgactg cgagctcgac accgcgctcc cccgtctcag
     6301 cctcgatcac gtcatagccg gcgtacgcca gcacgtcccg cacgagcttg aggttcttcg
     6361 cgttgtcctc gacgacgagg acccgacggc aactcacggc gcctcggaac tcgtccgcgt
     6421 caaagtgggt ggcagtacct ggatccgacg gagctgctcg accagttctt cacgcccgac
     6481 gggtttgatc aagtacgccg cggcccccat gccgaggccg cgttgtcgct cgtccacgac
     6541 cgagaccacg atgacgggga tgtgtgcagt cgcctcctcc gtcttgagca ccctgagcac
     6601 ctcccagcca tccatccgag gaagccggat gtcgagaatc accgcggcgg gcatcacccg
     6661 gcggaccgcc gccagcccgt ccactccgtc acgggccacc gtgacacgta tgtccaacga
     6721 tgcgaggaag acggtgagca ggtcgagcga gggtcggtcg tcctcgatga gcacgacctc
     6781 gacccggcct cgatccgggg ctggctctga cacgccggcg gcgaccgtag cgcggctgcc
     6841 cagtggaacg gagaacgaga aggtgctgcc gactccgacc tcgctgtcca gccacaactg
     6901 tccgccgcag agctcgacga tccgccgtga cagcgtcagg ccgagaccag tcccctcctc
     6961 gctcgacggc ccccgaccac cctgctggaa cgactcgaag atccgttcgc ggtcaggagc
     7021 ctcgacaccg ataccggagt cggcgaccga caccaccagg taaccgccct cgatggcggc
     7081 ccgggcgatc acagccccac cgtcgggcgt gaacttgacg gcgttgctca gcaggttcac
     7141 cacgacctgc ctgaaccgga gctcgtcggt gtcgacgagt ccgacgtcgg cggcgcactc
     7201 gatcgtcagg gtgatgccgt gcaccgtggc tcgttcgcga acgagcgaga cgccgtactc
     7261 caacgcagcg cggacgtcga cctcggagat gtccagctcc atccgcccgg cctccacctt
     7321 ggaca
//
LOCUS       DATBFZ010000228         1686 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_6310810, whole genome shotgun sequence.
ACCESSION   DATBFZ010000228 DATBFZ010000000
VERSION     DATBFZ010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6310810"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..339)
                     /locus_tag="VH419_06880"
     CDS             complement(82..339)
                     /locus_tag="VH419_06880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043374.1"
                     /translation="MTRPEPASAGSGAHRARSASVEQATVWSFDETTRQGEVVFDDGR
                     RLRFDAEAFAASGLRLLRAGQRVRLERDGDAVRRLTVLTLP"
     gene            complement(344..1081)
                     /gene="cofC"
                     /locus_tag="VH419_06885"
     CDS             complement(344..1081)
                     /gene="cofC"
                     /locus_tag="VH419_06885"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020574797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HEY7043375.1"
                     /translation="MSSKVARSTVMSSTPLNPAARHRLDAVSDVTDAPPTHWSVIVPV
                     KQTSLGKSRLAGLRAEQRRALALAFALDTVTAAASTVGVRRVVVVTNDPDASAFERIG
                     ADVLPDRPDAGLNPAFVHGAQHVAQIDPESGVLGLAGDLPALTTEVLGSVLRVAPSGR
                     WFVPDAAGTGTTLLAASSEMRLSPSFGPHSRAAHRAGGAQEVDLPGIARLRRDVDTEV
                     DLWDAVRLGVGAHTQEALSRHELLKLA"
     gene            1164..>1686
                     /locus_tag="VH419_06890"
     CDS             1164..>1686
                     /locus_tag="VH419_06890"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY7043376.1"
                     /translation="MARKRSQQKKGWAFGLCAFVLRRPLMVLTKRDWRGIEHIPETGG
                     CILVTNHVSEVDPVPFAHFVYDNGRLPRFLGKAEVFKVPVLGRIISNAGQIPVYRHSS
                     DASKAFSAAVDAVHRGECVIVYAEGTITRDPDMWPMVGKTGAARIALSTGCPVIPCAQ
                     WGPQDLLAPYARKP"
BASE COUNT          273 a          590 c          576 g          247 t
ORIGIN      
        1 ctcggtatca gatcagcggc caggccaccg cctggtcagc agcagagtca ccgcgaggcc
       61 accggcaacg tcatggcaac gtcatggcaa ggtcaacacg gtcagccggc gcacggcgtc
      121 accgtcgcgc tcgagccgca cccgctggcc cgcccggagc agccgcagcc cgctggcggc
      181 gaacgcctcg gcgtcgaacc gcagccgacg tccgtcgtcg aacacgacct caccttgtct
      241 cgtggtctcg tcgaacgacc acaccgtggc ttgctcgacc gacgccgaac gtgctcggtg
      301 cgcgccactg ccggccgatg ccggttccgg tctcgtcacg gcttcacgct agcttcagca
      361 gctcgtgtcg tgacagcgcc tcctgcgtgt gcgctccgac gcccagccgc accgcgtccc
      421 acaggtcgac ctcggtgtcg acgtcgcgtc ggagccgtgc gatgccgggc aggtcgacct
      481 cctgcgcacc acccgctcga tgcgcagccc gcgagtgcgg accgaacgac ggggacagcc
      541 gcatctcgga cgaggcggcc aacaacgtcg tgcccgtccc ggcggcgtcg gggacgaacc
      601 agcgaccgga cggtgcaacg cgcagcaccg aaccgaggac ctccgtggtc agggccggca
      661 ggtcgcccgc gagacccagc acaccggact cggggtcgat ctgcgcgacg tgttgtgcgc
      721 cgtgcacgaa cgccgggttg agccctgcat cgggccggtc gggcaacacg tcggcaccga
      781 tccgctcgaa ggcggacgcg tccgggtcgt tcgtcacgac cacgacccgg cgtacgccga
      841 cggtcgaggc cgcggcggtc accgtgtcga gcgcgaacgc caacgccagg gcgcgtcgct
      901 gctcggcacg gagtcccgca agccgtgact tcccgagcga ggtctgcttg acggggacga
      961 tcacggacca gtgcgtcggg ggcgcgtcgg tgacatcgct cacggcgtcg aggcggtgac
     1021 gagcggcggg gttcagcggc gtcgacgaca tcaccgtcga tcttgccacc ttgctcgaca
     1081 tcgacctccg ggtggacaaa actgtggacc ctgccgatga cccggactcg cgggcgactc
     1141 gggtggccga atgggagtca agggtggcga ggaagagatc gcagcagaag aagggctggg
     1201 ccttcggact gtgcgcattc gtgctgcggc gtccgttgat ggtgctgacc aagcgcgact
     1261 ggcgaggcat cgaacacatc ccggagaccg gcggctgcat cctcgtcacc aaccacgtct
     1321 ccgaagtcga cccggtgccg ttcgcgcact tcgtctacga caacggcagg ctgccgcgtt
     1381 ttctcggcaa ggcggaagtg ttcaaggttc ccgtgctcgg gcgcatcatc agcaacgccg
     1441 gccagatccc ggtgtaccgc cactcgtccg acgcctcgaa ggcgttcagt gctgctgtcg
     1501 acgcggtgca ccggggcgag tgcgtcatcg tgtatgccga agggacgatc actcgtgatc
     1561 ccgacatgtg gccgatggtc ggcaagaccg gggcggctcg gatcgccctc tcgaccgggt
     1621 gtccggtgat cccctgcgcg cagtggggtc cgcaagatct gctcgcgccg tacgcacgca
     1681 agccga
//
LOCUS       DATBFZ010000229         9023 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_2771086, whole genome shotgun sequence.
ACCESSION   DATBFZ010000229 DATBFZ010000000
VERSION     DATBFZ010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 9023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9023
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2771086"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1247)
                     /locus_tag="VH419_06895"
     CDS             complement(<1..1247)
                     /locus_tag="VH419_06895"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY7043377.1"
                     /translation="MAGSVLRAASLEWRSAAALERVRDERLRALLTHASTHVPYYRET
                     LDPDVVRTVRSAHQLSSLPVLERSTLQSERVHRMMSDQLVHTACHEAATSGSTGMPRR
                     VYFSEREMGHLRAAYAWDMLRLGVQPRDRVVQVRPLPFRGHSLERFGILPMLHIDTRQ
                     AVDLQVEQLLALRPTVVSAFPSVLLDIVEELERRGERYDDAHTVIFAGEPLRPVVRDR
                     VLGALGARYAETYSTVEIHTIARSCPRGALHLRTANVVVEVEHDDGSVSVADGTGDIV
                     VTRLLSDAMPLVRYRLGDRVEIGPDDCGCSVRSPIVHRVMGRIEDRLIDRAGRRVDAL
                     HLVPLLVRIPHIRLHQMQQREPGKVEIHVVPGPAAPCTLTDEVTRATAAAADLFDISV
                     HLVPDVLREPSGKIRVVKDLTNQ"
     gene            complement(1310..2632)
                     /locus_tag="VH419_06900"
     CDS             complement(1310..2632)
                     /locus_tag="VH419_06900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043378.1"
                     /translation="MTEPTDNSPASSPSVLHHDGNRDDQWLRPARVLGLVAGRAVFRL
                     TTYGAGIVLLAIWGATEFASFAAPVGATGWVLIAVGGIEKAAQTFLPRDPRRVLERFF
                     LLAAIVPYVLLVLVSVVVSAASPDSELATYVAAAALACGVGTASATVALFRMRDRPGL
                     DALTYLHLSVGYVGCVVVVAWLGLGVLPLFMLLLGWQTFAVGVLLARFLGNRIGVGGN
                     ASLRWEALRAIPLLSAGDLASAVGVSVLYVELSWGADQRELSTFYVAAVLLTVAAMVW
                     TYLLRLAQPTLVRRLASGKGHRDVSLGRRVASLSAATGIIVTVAAAAMAWWAGPDEMV
                     LVAAVVVLEPLLYIGASLGIFVLENFDARSRRLAAAAAIPELVTVVVVGAALAPRWGA
                     TGALVAVCAGQTAKSAAAFAVLSSVDPAQAATAPRSVAQTSTAGGAAR"
     gene            2728..3777
                     /locus_tag="VH419_06905"
     CDS             2728..3777
                     /locus_tag="VH419_06905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043379.1"
                     /translation="MAELEVTFHQLDTDPPPGWAAYRKSAGLTCAWDWSLMRARAADR
                     KTPHLALLVRDGDTPVGIGAARLAGPLLPGAWSSHRLLGGVLDVDCLVTSAVPGLQVD
                     GDAAGYEAALQGLRRETRRRFGHRVPAILLRQVPEHLLSTATRFPSVVLEAPPIGRHT
                     LAYESFDAYLRTLSRSRRSEFHQLERDPDVLVTYTGRGDPRPQLDVETALMLANEPVH
                     RHHAKWWMPKRLTGPALLTAAANHPEVDVITYRRRTGGLLGYGLVLAGGDWPISWMAG
                     TLPHGTGFPRGMWFHRELLYTRHWIEAGVQGYISGAGADSSKVRLGHQMVPNWSVLHP
                     LVGGDAPRFLRNATT"
     gene            complement(3757..5055)
                     /locus_tag="VH419_06910"
     CDS             complement(3757..5055)
                     /locus_tag="VH419_06910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043380.1"
                     /translation="MSTGLGVSVRELTTLPAVLRAPHHPRAQIEADALAGIQRMLTFA
                     RRQVPYYRDNADYARDISELSELAGLPILDKPQVLGAGVERFHVVSSGAFHVDRSSGT
                     TGRVIEVRHDDAAYGYHGATVLRRFLVSGYRPWWPIVQLKPFPRPVRWFQRLGLFPRT
                     VVDSALPADELARRVLASRPRVLMGYPVVLRALLRALGDSELTELRRRLRLVLTDSEL
                     LSSEGRRLLEDGFGTLVLDEYSAYEVLTVSSQCRSGSMHVDEDRVWLEIVDDDGEPVP
                     DGVEGRVVVTHFRERAMPLVRYALGDRAVRRSDGCACGMTTATMELSAGRSDDVVVLP
                     DGQHVYTGTFLGLAMFTPGVAESMVHQYADGAISIALVVDERSGLSFDEVAAEYSRRL
                     DGHVGQHVDAVFGEAANVTITPGGKARFVTSEYRSGGRVA"
     gene            complement(5052..6260)
                     /locus_tag="VH419_06915"
     CDS             complement(5052..6260)
                     /locus_tag="VH419_06915"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEY7043381.1"
                     /translation="MSTLLVLGGADGALGTIGAASRLGVRTLCVDSRTDAPGIARADE
                     FLSVSTRDIGAIERAIAGRDDLQGVISPASDVNLPAQFELAQRLGLPNGLSIDAVRAS
                     IDKGFFRDVCARIGLVNPSYVQGTFDHVLCHSNALRWPVMVKPADSSGGRGVSRCDNV
                     AQLAAACIEAAAWSRSRTVIVEEFVAGDDVGAEAFVIDGRVALLAIGSRTLTDRPHFV
                     TTETRVALPDQRWEAVRRCLDAICAELGYRWGSLNADLIVRPDGAVVVVEIGARLGGN
                     GSAELLGLAHGIDASEVAVELAVGAKPEVRPVLHRHAASRILGAPSEGKLVAIDGVGA
                     ARAVPGISQVVLAAEAGDHVAPYTKAGAKLGYLLAAGATADAVGKALDRAHRLLTVRV
                     AHPDLDRSEQ"
     gene            complement(6257..7273)
                     /locus_tag="VH419_06920"
     CDS             complement(6257..7273)
                     /locus_tag="VH419_06920"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY7043382.1"
                     /translation="MLQQADADVALGPLESNRFASGGEDMSLCVVIPCLNEQDNVEAA
                     YLEAITELAAYEPLEVLFVDDGSTDGTLEVIKELARRDSRVTYVSFTRNFGLEAAFSA
                     GYRYAHRPWILHLDADLQFPASTAHRMVAQARNGFDAVFGVRTQRHDPWHRRLSSLVH
                     NSIARRLLGIEMPAHATSFRLVRTSLARTIVDLELGTPYFLATLPQLTRRWTTVSTEH
                     RARAHGRSKVTFGGLARHALGLFMGFSSRPLLSAAGAGLLASIAFALAVIAGAVSSPT
                     GTLAGLGAGGVFMGWGIAMLSGSALLIRRDQRRTPPFLIAEANFALRPQDDLQRNDHR
                     ALTA"
     gene            7453..>9023
                     /locus_tag="VH419_06925"
     CDS             7453..>9023
                     /locus_tag="VH419_06925"
                     /inference="COORDINATES: protein motif:HMM:NF014589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase N-terminal
                     domain-containing protein"
                     /protein_id="HEY7043383.1"
                     /translation="MYVLGINQHHDSAAGLVRDGQIIAFCEEERLSRRKHDGDFPARA
                     IQYCLDEGGITLADVDRVAYFWQGHRELLHAAEHFARHFPDTLAVLRNEGGYFTPRAQ
                     SLRETFLNRGAQRHPDDYHVGGALLLHFRRSLGIKTLVREAVGFEGPTRFKVDLVDHH
                     RAHAASTYLLSPWHDSAIITWDGIGSDGTSTFLARGDGDTIVPIRRVKFPHSLGAFYA
                     DVTGWLGFNPTRDEGKVMGLAAYGEPTFVDDFRSLVRLEDDGHYALDLSWFRHHASGK
                     HQVSSRFLSAFGPPRERGGHDVPQHYADVAYALQVTLEEVGLHVARWLQAETGATKLC
                     TAGGVALNSVMNGRILLETDFVDFFAQPASADDGSALGAALAVAVEDHHAPRHRGGYI
                     FLGPRYTDNEIEKAIVHAGLPYERVDDVGAVVAQSIADGNIVGWFQDRMEAGPRALGN
                     RCILADPRDPTAKDRLNAKVKHREPFRPFAPSCLAERSGEYFASDYPSPVMLLVFDVL
                     EHQRAAVPAITHHDGT"
BASE COUNT         1527 a         3131 c         3022 g         1343 t
ORIGIN      
        1 ctctggttgg tcaggtcctt gacgacccgg atcttgccgc tcggttcccg gaggacgtcg
       61 gggacgagat gaacgctgat gtcgaacagg tccgccgcag cggccgtcgc ccgggtcacc
      121 tcgtccgtca aggtgcaagg cgcggccggg ccggggacga cgtggatctc gaccttcccg
      181 ggttcgcgct gctgcatctg atgcagccgg atgtgcggta tccggaccag cagcggcacg
      241 agatggagag cgtcgacacg acgcccggca cggtcgatga gccggtcctc gatccgcccc
      301 atgacgcgat gcacgatcgg gctgcgcacg gagcatccgc agtcgtcagg gccgatctcg
      361 acccgatcgc cgagccggta gcggaccagc ggcatcgcgt cggacagcag ccgggtcacg
      421 acgatgtcgc cggttccgtc tgccaccgag accgagccgt cgtcgtgttc gacctcgacc
      481 acgacgttgg cggtgcgcag gtgcagcgca ccccgaggac acgagcgggc gatcgtgtgg
      541 atctcgaccg tcgagtacgt ctcggcatac cgggcgccga gcgctccgag gacacggtcg
      601 cgcacgacag gcctcagcgg ttcgcccgcg aagatgaccg tgtgtgcgtc gtcgtaccgc
      661 tcgcctcgtc gctccagctc ctcgacgatg tccagtagga cactgggaaa cgccgacacc
      721 acggttggcc gaagtgccag caactgctcc acctgaaggt cgacggcctg ccgagtgtcg
      781 atgtgcagca tcggcagtat gccgaaacgc tcgaggctgt ggccgcggaa cggcagcggc
      841 ctgacctgca cgactcgatc acggggctgg acaccgagcc ggagcatgtc ccaggcgtac
      901 gccgcccgga ggtgccccat ctctcgttcc gagaagtaga cgcgacgtgg catgccggtc
      961 gagccgctgg tggccgcttc gtggcaggcg gtgtggacga gctggtccga catcattcgg
     1021 tggacccgct cgctctgcaa cgtcgaccgt tcgagcaccg gcagggatga cagctggtgt
     1081 gccgatcgca ccgtccggac gacgtccgga tcgagcgtct cgcgatagta cgggacgtgg
     1141 gtgctggcgt gcgtgagcaa ggcccgcagt cgctcgtccc gtacccgctc gagcgcggca
     1201 gccgaacgcc actccagtga cgcggctcgc aagacgctgc ctgccacggg caagaccgga
     1261 atcgagcgag ggatgcgcgg cgtcgatccg cgaagggaag caaggcggtt catcgggccg
     1321 ccccacctgc cgtcgaggtc tgcgcgaccg accgaggcgc agtagcggcc tgcgccggat
     1381 cgacggacga caggaccgca aacgccgccg cagacttcgc cgtctggcct gcgcagaccg
     1441 ccaccagcgc cccggtggcg ccccatcgtg gcgcgagtgc tgcgccgacc acgacgaccg
     1501 tcaccagctc gggaatcgcc gcggctgccg ccagacggcg ggagcgggcg tcgaagttct
     1561 cgagcacgaa gatgccgagc gaggcaccga tgtacagcag cggttcgagc accacgaccg
     1621 ccgcgaccag gaccatctca tcggggcccg cccaccaggc catcgccgct gcggccaccg
     1681 tcacgatgat tcccgtcgcg gcggaaagcg acgccacccg gcgaccgaga ctcacgtccc
     1741 ggtgcccctt gcccgaggcg agacgccgca ccagggtcgg ctgggccaac cgcagcaggt
     1801 acgtccagac catggccgcc accgtcagca ggaccgcggc gacgtagaac gtgctgagct
     1861 cgcgctggtc ggcgccccag gacagctcga cgtacagcac tgacacaccg actgccgacg
     1921 cgaggtcgcc tgcactcagc agcgggatcg cccgcagtgc ttcccatcgc agcgatgcgt
     1981 tgccgccgac gccgatccgg ttcccgagga agcgcgccag gagtacgccg acggcgaagg
     2041 tctgccagcc gagaagcagc atgaacagcg ggaggacccc gagaccgagc cacgcgacga
     2101 cgacgacgca gccgacgtat cccaccgaca ggtgcaggta cgtcaacgca tcgagcccgg
     2161 gccggtcacg catgcggaac agcgcaacgg ttgccgacgc cgtgccgact ccgcacgcca
     2221 gtgcggctgc tgccacgtag gtcgccaact cggagtccgg cgacgcggcg ctcacgacga
     2281 cggacacgag cacgagcagc acgtacggaa cgatcgccgc cagcaggaag aaccgctcga
     2341 gcacgcgccg tggatcacga ggaagaaacg tctgcgcggc cttctcgatg cctccgaccg
     2401 cgatcaagac ccatccggtt gcccccaccg gagctgcgaa cgaagcaaac tcggtggcgc
     2461 cccagatcgc gagcagcacg atgcccgcgc cgtaggtcgt gaggcgaaac actgcacgtc
     2521 ccgcgaccag gccgagtacc cgtgctggtc gcagccactg atcgtcccgg ttgccgtcgt
     2581 ggtgcaggac gctcggcgat gatgctggac tgttgtcggt cggttccgtc atgaccccac
     2641 cccgaccggg atgccgaacg agaccgcttg tcgtgccata tcttcgtggg gactcggtga
     2701 agcggggtcg acagaggggg tgtcgccgtg gcagagctgg aggtgacctt tcatcagctc
     2761 gacacagacc cgccgccggg ctgggcggcg taccggaagt cggctggact gacctgcgcc
     2821 tgggactggt ccctgatgcg tgcgcgggcg gcggaccgca agacacctca cctcgcgctg
     2881 ctcgtcaggg acggcgacac accggtcggg atcggtgccg cccgcctggc cggcccgctc
     2941 cttcccggtg cgtggtcgag ccaccggctg ctggggggcg tcctcgacgt cgactgcctg
     3001 gtcacatctg ccgtgcccgg cctgcaggtc gacggggatg ccgcgggata cgaggcggcg
     3061 ctgcagggcc tgcgtcgcga gacccgccgt cgattcggac accgggtacc ggcgatcctg
     3121 ctgcgccagg tgccggaaca cctgttgtcc acggcgaccc ggttcccgtc cgtggtgctc
     3181 gaggcgccgc cgatcggccg gcacaccctc gcatacgaga gcttcgatgc ctacctgcgg
     3241 accctttcgc gctcccgacg cagcgagttc caccagctcg aacgcgatcc ggacgtcctc
     3301 gtgacgtaca ccggaagggg cgacccgcgg cctcagctcg acgtcgagac ggcactgatg
     3361 ctcgccaacg agcctgtcca ccgccaccac gccaagtggt ggatgccgaa gcgcttgacc
     3421 ggacccgccc tcttgaccgc cgcggccaac catcccgagg tcgacgtcat cacctaccgg
     3481 cgccggaccg gcgggctgct gggctacggc ctggtcctgg ccggcggtga ttggccgatc
     3541 agctggatgg caggcacgtt gccccatggc actggcttcc cgcgtggcat gtggttccat
     3601 cgcgagctgc tgtatacaag acactggatc gaggccggcg tgcagggata catcagtggt
     3661 gccggcgccg actcgtccaa ggtccggctc ggtcatcaga tggtccccaa ctggtctgtg
     3721 ctccacccgc tcgtgggggg cgacgcaccg cggttcctac gcaacgcgac cacctgaccg
     3781 gtactcgctg gtcacgaacc tcgctttgcc accgggggtg atcgtgacgt tggccgcctc
     3841 gccgaacacc gcatcgacat gctgtcccac gtgaccgtcg agtcgcctgc tgtactccgc
     3901 ggccacctcg tcgaagctga ggcctgaccg ttcgtcgacc acgagcgcga tcgatatggc
     3961 gccgtcggcg tactgatgga ccatgctctc tgcgacgcct ggggtgaaca tcgccaagcc
     4021 gaggaatgtc ccggtgtaga catgctgccc gtcggggagg accacgacgt cgtcggatcg
     4081 tcccgcgctg agctccatcg tggccgtggt catgccacag gcacatccgt ccgaacggcg
     4141 taccgcccgg tcgccgagcg catagcggac cagcggcatc gcccgctccc ggaaatgcgt
     4201 gacgacgacg cgtccctcga cgccatcggg aaccggctcg ccatcgtcgt cgacgatctc
     4261 cagccagacg cggtcctcgt cgacgtgcat cgacccggat cgacactgcg acgacacggt
     4321 gagcacctcg tacgcggagt actcgtccag caccaaggtc ccgaagccgt cctccagcag
     4381 ccgccggccc tcggagctca gcagctcgga gtcggtgagc accagccgca gccgtcggcg
     4441 cagctccgtc agctccgaat cgccgagggc gcgcaacagc gctcgcagga ccacgggata
     4501 tcccatcaac acccgcggac gacttgcgag gacccggcga gccagctcgt cggccgggag
     4561 cgcagagtcg acgacggtcc gggggaacag gcccagccgc tggaaccacc ggaccgggcg
     4621 cgggaacggt ttgagctgga cgatcggcca ccacggccgg tagcccgaga ccaggaagcg
     4681 ccgcagcacc gtggcgccgt gatagccgta cgccgcatcg tcgtggcgca cctcgatcac
     4741 tcggcccgtt gtgccactgc tccggtccac atggaaggct ccgctcgaaa cgacgtgaaa
     4801 ccgctcgaca cccgcgccga gcacctgcgg tttgtcgagg atcggcagtc cggccagctc
     4861 gctcagctcg ctgatgtcac gtgcgtagtc ggcgttgtcg cggtagtacg gcacctgccg
     4921 gcgtgcgaag gtgagcatcc gttgaatgcc ggcgagcgcg tcagcctcga tctgggcccg
     4981 cgggtgatgt ggtgcgcgca gcaccgcagg caaggtcgtc aactcgcgca cggacacgcc
     5041 gaggccggtg ctcactgttc gctccggtcc aggtctgggt gagcaactcg caccgtcagc
     5101 agacgatgtg ctcgatcgag tgccttgccg acggcgtccg ccgtcgcacc cgctgccagc
     5161 aggtacccga gcttggcgcc ggccttggtg tacggcgcga cgtggtcccc cgcctcggcc
     5221 gccagcacga cctgggagat cccggggacg gctcgagcgg cgcccactcc gtcgatcgcg
     5281 accagtttgc cttcggatgg cgcgccgagg attcgggacg ccgcgtggcg gtgcaggacc
     5341 ggccggactt cgggcttggc accgaccgcc agctcgaccg caacctcgct ggcgtcgatg
     5401 ccgtgagcga ggcccagcag ctctgccgag ccgttgccgc cgagccgtgc cccgatctcg
     5461 acgaccacca ctgcaccgtc aggcctgacg atgaggtcgg cattcagaga tccccatcga
     5521 tagccgagct cggcgcagat ggcgtcgagg caccgcctca ctgcctccca ccgttgatcg
     5581 gggagtgcga cgcgtgtctc ggtcgtgacg aagtgcggcc tgtcggtcag ggttctcgat
     5641 ccgattgcca gcagcgcgac ccggccgtcg atgacgaacg cctcggctcc gacatcgtca
     5701 cccgcgacga actcctccac gatgacggtc cgcgatcgtg accaggccgc cgcttcgatg
     5761 caggcggccg cgagctgagc aacgttgtcg catcgtgaca cgccgcgccc accgctggag
     5821 tcggccggct tgaccatgac cggccaacgc agcgcgttgc tgtggcacaa gacgtggtcg
     5881 aaggtccctt ggacgtacga cgggttgacc aggccgatcc gagcgcagac gtcgcgaaag
     5941 aaccccttgt cgatcgatgc acgaaccgcg tcgatcgaga gaccgttggg gagtccgagt
     6001 cgttgcgcca gttcgaactg ggccggcagg ttgacgtcgc tcgccggtga gatcactccc
     6061 tgtagatcgt cgcggcccgc gatggctcgt tcgatggcac cgatgtcccg agtgctgaca
     6121 ctgaggaact cgtcggcgcg tgcgattccg ggcgcatccg tccggctgtc gacacacagc
     6181 gtccgaacgc cgagtcggct cgccgccccg atggtcccga gcgcaccatc cgcgccgccg
     6241 agcaccagca gcgtgctcat gctgtcaacg cccggtggtc gttgcgctgg aggtcgtcct
     6301 gcggccggag cgcgaaattg gcctcggcga tgagaaacgg gggagtacgc cgctggtctc
     6361 ggcggatgag gagggcagac ccgctcaaca tggcgatgcc ccaccccatg aacaccccac
     6421 cggccccgag accggcgagc gtcccggtcg gggagctcac agctccggcg atgacggcca
     6481 gcgcgaaggc gatggatgcg agcagacccg caccagcggc gctgagcaac gggcgcgacg
     6541 agaaccccat gaacaggccc agtgcgtgtc gtgcgagccc gccgaaggtg actttcgacc
     6601 gaccgtgcgc ccgggctcga tgctcggtcg acaccgtggt ccagcgtcga gtcagctgcg
     6661 gcaaggtcgc gaggaagtat ggcgttccca gctcgagatc aacgatggtc cgggccaagc
     6721 tggtccggac gagccggaag cttgtcgcat gtgcaggcat ctcaatgccc agcagccgtc
     6781 gtgcgatgga gttgtgcacg agggacgaca aacgccgatg ccaaggatcg tgtcgttgcg
     6841 ttcggacgcc gaagacggcg tcaaagccgt tgcgagcctg cgcgaccatg cgatgcgccg
     6901 tgctcgcggg gaactgcaga tcggcgtcga gatggaggat ccacgggcgg tgcgcatagc
     6961 ggtagccggc cgagaacgcg gcctccaggc cgaagttgcg ggtgaaggac acataggtga
     7021 cccgggagtc ccgccgggcc agttccttga tcacctcgag ggtgccgtcg gtgcttccat
     7081 cgtcgacgaa cagcacctcg agcggctcgt atgcagcgag ctccgtgatg gcctccaggt
     7141 atgcagcctc gacgttgtcc tgctcgttga ggcacggaat caccacgcac agactcatgt
     7201 cctcgccgcc ggaggcgaac cggttcgact ccagcgggcc cagcgcaacg tcggcgtccg
     7261 cctgctgcaa cacgtgatcc cccaagaaac gatcgcgccg cgactggcga ggcggcccga
     7321 ttggtgatga gtgaaggaag caatatcgag cggttctgat accccggtgc atccagccac
     7381 cggtcagcgc ctcggcgtat caccagggtc cggatcgagt cttggtttca tgccaactgg
     7441 gggagcgaga gcatgtacgt cttggggatc aaccaacacc acgactcggc cgcgggcttg
     7501 gttcgcgatg gacagatcat cgcgttctgc gaggaagagc ggctcagtcg gcgcaagcac
     7561 gacggcgact tccccgcgcg cgcgatccag tactgcctcg acgagggcgg catcacactt
     7621 gccgacgtcg accgcgtcgc gtacttctgg cagggccacc gggagctgct gcacgcggcc
     7681 gaacacttcg ccagacactt tccggacacc ctcgccgtcc tccgcaacga gggcggctac
     7741 ttcacccctc gggcacagag cctgcgcgag acgttcctga accgcggcgc acagcggcat
     7801 cccgacgact accacgtcgg aggtgcactc cttctgcatt tccggcggtc cttgggaatc
     7861 aagaccctgg tgcgagaggc ggtcggcttc gaaggaccga cgaggttcaa ggtggatctg
     7921 gtcgaccacc acagggcgca tgcggcgagc acgtacttgc tctccccctg gcacgactcg
     7981 gccatcatca cctgggacgg catcggctcc gacggcacgt cgacgttcct cgcccgcgga
     8041 gacggcgaca ccatcgtgcc gattcggcgg gtgaagttcc cgcactcgct cggtgccttc
     8101 tatgccgacg tgacgggctg gctcggcttc aacccgaccc gcgacgaggg caaggtgatg
     8161 ggcctggcgg cgtacggcga accgaccttc gtcgacgact tccgctcgtt ggtgcggctc
     8221 gaggacgacg gccactacgc cctcgacctg tcgtggttcc gccaccacgc gtcaggcaaa
     8281 caccaggtca gctctcggtt cctctcggcc tttggacctc cgcgggaacg cggggggcat
     8341 gacgtgccgc agcactacgc ggacgtcgcc tacgccctcc aggtgacgtt ggaggaggtc
     8401 gggctccacg tggcgcggtg gctgcaggcg gagaccggcg ccaccaagct gtgcaccgca
     8461 ggtggggtgg cactgaacag cgtcatgaac ggaaggatcc tgctggagac cgacttcgtc
     8521 gacttcttcg cccaacctgc ctcggcagac gacggctcgg cgctgggagc agccctggcg
     8581 gtcgctgtcg aggatcacca tgcgccgcgt caccgtggag ggtacatctt cctcggaccg
     8641 cgctacaccg acaacgagat cgagaaggcc atcgtccacg ccggcctgcc ctacgagcgt
     8701 gtcgacgacg tcggagcagt ggtcgcgcag agcatcgccg acggcaacat cgtcggctgg
     8761 ttccaggaca ggatggaggc gggcccccgg gcgctcggca accgatgcat cctggccgat
     8821 cctcgtgacc ccaccgcgaa ggaccggctc aacgccaagg tgaagcaccg tgagccgttt
     8881 cggccgtttg ctccgtcgtg cctggccgaa cgctcgggcg agtacttcgc cagcgactac
     8941 ccgtcccccg tcatgctgct cgtcttcgac gtcctcgagc accagcgcgc ggccgttccg
     9001 gcgatcacgc accatgacgg cac
//
LOCUS       DATBFZ010000230         4653 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_1135529, whole genome shotgun sequence.
ACCESSION   DATBFZ010000230 DATBFZ010000000
VERSION     DATBFZ010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 4653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4653
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1135529"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="VH419_06930"
     CDS             <1..304
                     /locus_tag="VH419_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043384.1"
                     /translation="RIRDAVHLRSPVDCFPYATNTSRRMDLDHTVAYVDPGDGGPPGQ
                     TRPDNLGPMVRFHHRIKTHSSWQVWQPTSGVFVWRSPHGRYYLVDHTGTRPAYKTA"
     gene            complement(384..623)
                     /locus_tag="VH419_06935"
     CDS             complement(384..623)
                     /locus_tag="VH419_06935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043385.1"
                     /translation="MTTQDPHEGHYDDEEGPGGSHGGHTDEHEGHFDDVEEADGRHEG
                     HTDQHEGHFDDVEEPGGRHEGHTDQRQGHYDDVDE"
     gene            complement(742..1239)
                     /locus_tag="VH419_06940"
     CDS             complement(742..1239)
                     /locus_tag="VH419_06940"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEY7043386.1"
                     /translation="MTPDDVRAVMNDPVAERLLGSANPGRLGYVGRDGSPRVIPVAFH
                     WTGTTIVIGTVPGSAKVKALEANPEVALTVDTNPPVWPPNVLLLRGTATITLIDGVFP
                     EYVAASKKLIPDEAFPEWEAGVHALYDQMARIDITPTWAAIQDFETRLPRAVAELARA
                     KFGDA"
     gene            1381..2250
                     /locus_tag="VH419_06945"
     CDS             1381..2250
                     /locus_tag="VH419_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012923415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY7043387.1"
                     /translation="MRYGLDITAAGPWGRPDRIAELATLAERSGWDGVFCEDYICFPG
                     EGTDPVDTYDVWITLALVAQATTRVTLGTMVTPVPRRRPWVLASQALTVDHLSSGRLV
                     LGVGLGDPEPRNFASFGEVTDLAERAGMLDEALDIVARLWTGQRVSFEGRHYRIHDAQ
                     LRPRPVAVPRVPIWVGGALTLARPRRRALRWDGACLYRIPTQHGWEDVTPEDVRRLRA
                     DVDARPGGGAGYVIAVGGRERSLDDPEADREYVAALADAGADWWHEYVPPRLSYDEAR
                     RRIESGPVRAEPA"
     gene            2267..2869
                     /locus_tag="VH419_06950"
     CDS             2267..2869
                     /locus_tag="VH419_06950"
                     /inference="COORDINATES: protein motif:HMM:NF021079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trp biosynthesis-associated membrane protein"
                     /protein_id="HEY7043388.1"
                     /translation="MPPAQPPIRYAPTLVLGLLGAVALTVGVSKPWLTATAKQSSLPT
                     LHAEVTGADLAPIAGALGFVVLAAFGAVIATRGWVRRALGVLIVVAAAVVLVAAIDPP
                     GGTDSLRDGLTAKGWTSGSFDTATTWWRWLTIVGAIVCILAGLAVAAYGGRWATMGST
                     YDAPHPRPAADVVPPAEDLSEAELWREIDQGRDPTRRRES"
     gene            2936..3142
                     /locus_tag="VH419_06955"
     CDS             2936..3142
                     /locus_tag="VH419_06955"
                     /inference="COORDINATES: protein motif:HMM:NF041681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HGxxPAAW family protein"
                     /protein_id="HEY7043389.1"
                     /translation="MADHQQHETHHGSTPAAWTAVVLCLVAFLVAGAGLMIDNWVVFW
                     IGIGLILVSGVVGKIMQAMGLGAR"
     gene            3162..3971
                     /gene="trpC"
                     /locus_tag="VH419_06960"
     CDS             3162..3971
                     /gene="trpC"
                     /locus_tag="VH419_06960"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020388804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEY7043390.1"
                     /translation="MSILDEIIIGVREDLAVRQAQVDLDELKATAARQNSALDPMPGY
                     RAPGISVIAEVKRSSPSKGVLADIADPAALAADYEAGGASTISVLTEERRFGGTLDDL
                     RAVRAAVSIPVLRKDFIVTSYQLWEARAAGADLALLIVAALDDNELEGLVERARSIGL
                     TPLVEVHTEDEVPRALGAGADLIGVNARNLKTLEVDRDVFARLAPLIPDGVVRVAESG
                     VRGPHDVLEYARNGADVVLVGETLVTGKDPRTAVSDMVAAGTHPALKQRHA"
     gene            4142..>4653
                     /locus_tag="VH419_06965"
     CDS             4142..>4653
                     /locus_tag="VH419_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020388805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEY7043391.1"
                     /translation="MPDAAGHFGRFGGRFMPEALIAPLDELEQQWRAAIVDDTFTAEL
                     SRLQREYAGLPSLLYDAERFSEVVGARVLLKREDLNHTGAHKIRNVLGQALLTTRMGK
                     KRVIAETGAGQHGVATATACAYFGLECVVYMGEVDTERQALNVARMRLLGAEVTAVKS
                     GSRTLKDAMN"
BASE COUNT          730 a         1589 c         1566 g          768 t
ORIGIN      
        1 ccggatccgc gacgcggtcc atctgcggtc cccggtcgac tgcttcccgt acgccaccaa
       61 cacctcccgc cggatggacc tggaccacac cgtcgcctac gtcgaccccg gcgacggtgg
      121 accacccggc caaaccagac ccgacaacct cggaccaatg gtccggttcc accaccggat
      181 caaaacccac agcagctggc aggtctggca acccacctcc ggcgtgttcg tgtggcgatc
      241 accacacggc cgctactacc tcgtcgacca cacaggcacc cgccccgcat acaagaccgc
      301 ctagcgcacc gcgcaggtga gcggtcgacg tggagatctc ccggccctgg cgacctcacg
      361 ctgccggtca gcgacctgaa cgtctactcg tcgacgtcgt cgtagtggcc ctggcgttgg
      421 tcggtgtgtc cttcgtgccg gccgccgggt tcttcgacgt cgtcgaagtg gccctcgtgt
      481 tggtcggtgt gtccttcgtg ccggccgtcg gcttcttcga cgtcgtcgaa gtgaccttcg
      541 tgttcgtcgg tgtgcccccc gtgcgagcca ccgggtcctt cttcgtcgtc gtagtgacct
      601 tcatgcggat cttgggtcgt catgcctcaa ctatggaccc ctgaggtgag ccggtgccac
      661 gagtgagggt ccagcgccaa aaagccggga gtcgggccta gagacaaacc tcacgtcgcg
      721 tgctggcgac ttgtctcgat gtcaggcgtc gccgaacttg gctctggcga gttccgcgac
      781 cgctctcggg agccgggtct cgaagtcttg gatcgccgcc catgtcggcg tgatgtcgat
      841 gcgcgccatc tggtcgtaga gcgcgtggac gccggcctcc cactctggga atgcctcgtc
      901 tgggatcagc ttcttcgagg cggcgacgta ctcaggaaag acgccgtcga tcaaggtgat
      961 cgtggcggtc ccacgcagca acagcacgtt cggcggccac accgggggat tcgtgtcgac
     1021 ggtcagcgca acctccgggt tggcctccag cgccttgacc tttgccgacc cgggcacggt
     1081 gccgatcacg atcgtggtgc cggtccaatg aaacgccacc gggatcaccc ggggtgaccc
     1141 gtccctgccg acgtatccga gtctgccggg gttcgccgaa ccgaggagtc gctcggcaac
     1201 ggggtcgttc atcacggcgc gcacgtcgtc tggagtcatg tgctcacctc cgtgaggctt
     1261 cgttcaggcg gtccgccgac gcttcaacgt ctcgatcctc gcgctgttcg gctggccgca
     1321 ccaccgcgtc gtctccgccg atcaggagca ggtgtcggtg gtcggtgctc gactcgcgac
     1381 atgagatacg gactcgacat cacagcagcc ggtccctggg gacggcccga ccggatcgct
     1441 gagctcgcga cacttgccga gcgttcgggc tgggacggcg tcttctgcga ggactacatc
     1501 tgcttccccg gtgagggcac agatccggtc gacacgtacg acgtctggat cacactggcg
     1561 ctcgtggcgc aggcgacgac gcgcgtcaca ctcggcacga tggtgacacc agtccctcgt
     1621 cggcggccat gggtgctcgc ctcccaggcg ttgacggtcg accacctgtc cagcggccga
     1681 ctcgtgctgg gggtcggtct gggagatccc gagccacgca acttcgccag tttcggtgag
     1741 gtgacggatc tggccgagcg ggcaggcatg ctcgacgagg cgctcgacat cgtggcgcga
     1801 ctgtggactg ggcagcgcgt gtcgttcgag ggtcggcact accggatcca tgacgcacag
     1861 ctgcgccctc gaccggtcgc agtcccgcgc gtgccgatct gggtgggcgg cgcgctcacg
     1921 ctggccagac ctcggcggag ggcgcttcgc tgggacgggg cctgcctgta ccggattccg
     1981 acacagcacg gctgggagga cgtgacaccg gaagacgtcc gtcgactcag agccgacgtg
     2041 gacgcgcgac ctggcggcgg cgccggatat gtgatcgccg tgggcggacg cgagcggtca
     2101 cttgacgacc cggaagcaga ccgcgagtac gttgcagcgc tggccgacgc cggagccgac
     2161 tggtggcacg agtacgttcc gccacggttg tcatacgacg aggcacggcg gcgcatcgag
     2221 tcaggcccgg tgcgtgccga gccagcgtga cgacgagaat ggcgacatgc cgccggcgca
     2281 gccccccatc cgttacgcgc caaccctggt gctcgggctg ctcggggccg tcgccttgac
     2341 ggtgggtgtc agcaagccgt ggttgaccgc gacggcgaag cagagcagtc tccccacgct
     2401 gcacgctgaa gtgaccggcg cagacctggc gccgatcgct ggagccctgg ggttcgtcgt
     2461 gctcgcggcc ttcggcgccg tgatcgcgac ccgcggctgg gtgcgccgtg cactgggcgt
     2521 gctcatcgtg gtcgcggcgg cggtcgtgct ggtcgcggcg atcgacccac ctggagggac
     2581 cgacagtctg cgtgacgggc tcaccgccaa gggctggaca tccggcagct tcgacaccgc
     2641 cacgacctgg tggcggtggc tcacgattgt gggggcgatc gtctgcatcc tggccggcct
     2701 cgcggtcgcg gcgtacggcg gccggtgggc cacgatgggc agcacgtacg acgccccaca
     2761 cccgcgcccg gcagccgacg tggttcctcc ggctgaggat ctcagcgagg ctgagctgtg
     2821 gcgcgagatc gaccagggca gagacccgac ccgccgtcgt gagagctgac ccgtcacaat
     2881 actgttcgag acggtcgagg ctcagaccat cgcctcggcg agaggagtac gacagatggc
     2941 cgaccaccag cagcacgaaa cccaccatgg atcgacgccc gctgcctgga cagcggtcgt
     3001 gctgtgcctg gtcgcgttcc ttgtcgccgg tgccggcctc atgatcgaca actgggtggt
     3061 gttctggatc gggatcgggc tgatcctcgt gtccggcgtc gtcggaaaga tcatgcaggc
     3121 gatgggtctt ggggcccgct gacgacggcg acggtgctgt gatgtcgatc ctcgacgaga
     3181 tcatcatcgg cgtacgcgag gatctcgcgg tccgccaggc gcaggtcgac ctcgacgagc
     3241 tcaaggcgac cgcagcgcgg cagaactccg ctctcgaccc gatgcccggt tatcgggcac
     3301 caggcatctc ggtgatcgcc gaggtcaaac gctccagccc gagcaagggc gtgctcgccg
     3361 acatcgccga tcctgctgct ctcgccgccg actacgaagc cggcggcgcg tccacgatca
     3421 gtgtgctgac cgaggagcgc cggttcggcg gcacgctcga cgatctacga gcagtccggg
     3481 cagcggtgag catccctgtc ctccgcaagg acttcatcgt gacgtcgtac cagctgtggg
     3541 aggcaagggc cgccggcgcc gaccttgctt tgctcatcgt cgcggctctc gacgacaacg
     3601 agctcgaagg cctggtcgag cgggcacgtt cgatcgggct gacaccgctc gtcgaggtcc
     3661 acaccgagga cgaggtacca cgagcgctcg gcgccggggc cgatctgatc ggcgtcaacg
     3721 cacgcaacct gaagacgctc gaggtcgacc gcgacgtgtt cgcccggctc gcgccgctga
     3781 tccccgacgg cgtcgtcaga gtcgccgagt ccggcgtccg gggcccacac gacgtcctcg
     3841 agtacgcgcg caacggcgct gacgtcgtcc tcgtcggcga gacactggtc accggcaaag
     3901 acccgcggac ggcggtgtcg gacatggtgg ccgctggaac acatcccgcg ctcaagcaaa
     3961 ggcacgcctg aatggcctgt gccccgtcga ccaccattcg ctctgatgcg cgacgcccga
     4021 gcggcgatct gcggcgttct cgtcgtcgct cgatgctggg catcgatctc ctcctcggcc
     4081 ttgcatctca tccccccgga cgccgctctt gacgagcaag ggcggccgac gaggcaacac
     4141 catgcccgac gcggctgggc acttcggccg cttcggtgga cggttcatgc cggaggcgct
     4201 gatcgcgccg ctggacgagc tcgaacagca gtggcgtgct gcgatcgtgg acgacacgtt
     4261 caccgcggag ctctctcggc tgcagcgcga gtacgccggt ctgccctccc tgctctacga
     4321 cgccgagcgg ttctcggagg tggtcggtgc ccgggtcctg ctcaagcgcg aggacctcaa
     4381 ccacacgggc gcacacaaga tccgcaacgt cctcggtcaa gcactgctca cgacccggat
     4441 gggcaagaag cgcgtcatcg ccgagaccgg tgcaggccag cacggcgtcg caaccgccac
     4501 cgcatgcgcc tacttcggtc tcgagtgcgt cgtctacatg ggcgaggtcg acaccgaacg
     4561 tcaggcactc aacgtcgcgc gaatgcggct gctcggtgcc gaggtcacgg cagtcaagtc
     4621 cgggtcacgg accctcaagg acgcgatgaa cga
//
LOCUS       DATBFZ010000231         4700 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_2119170, whole genome shotgun sequence.
ACCESSION   DATBFZ010000231 DATBFZ010000000
VERSION     DATBFZ010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 4700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4700
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2119170"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1354
                     /locus_tag="VH419_06970"
     CDS             <1..1354
                     /locus_tag="VH419_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008357204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HEY7043392.1"
                     /translation="QNQWISSLDVVAHEHGHGVDDHTPGGISGNGTQEFVADTFGAAT
                     EWYANEPSSYDPPDFTVGEEVNLVGSGPIRYMYNPSLAGDDNCYSSSIPNEEVHAAAG
                     PGNHWFYLLAEGSSPTNGQPTSPTCNGSSVTGVGIQKAEQIMYNAMLMKTSSSSYLKY
                     RTWTLTAAKNLDATCGLFNATKAAWNAVSVPAQSGDPTCTGGGGNNTVTVNNPGNQSG
                     TVGTATSLQMTGTDSQSGQTLTWSATGLPAGLSINASTGLISGTPTTANTYTPTVTAT
                     DTTGAHGSTSFTWTIGGGGGGGCSSPGQKLGNPGFETGSASPWSASAGVIDSSSAEPA
                     HSGSWKAWLDGYGRTHTDTLSQSVTIPAGCAATLSFWLHIDTSETTTTTAYDKLTVKA
                     GSTTLATYSNLNKNTGYSQKSFDLSAFAGQTLTISFTGVEDVSLQTSFVIDDTALTVS
                     "
     gene            1501..1916
                     /gene="rnpB"
                     /locus_tag="VH419_06975"
     ncRNA           1501..1916
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VH419_06975"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(2719..3288)
                     /locus_tag="VH419_06980"
     CDS             complement(2719..3288)
                     /locus_tag="VH419_06980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043393.1"
                     /translation="MALWIGLEEENMSGNGLVQGGLFHDRDGQGTPHMCKFYEKYPAQ
                     SAHLYDCGDFNGGAYYSFIVKKYNTPSGKMVEVLDCGEWDSDPYGTCVTELSGISDNY
                     ILYDHAIASAEETHKYCQDTMFGNSTSPANIGSSSKPIEVEHDSGDPWIDTETFDGVP
                     QDGAYTCDHFHKDLPNPQTLVVWDDRNVD"
     gene            3807..>4700
                     /locus_tag="VH419_06985"
     CDS             3807..>4700
                     /locus_tag="VH419_06985"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEY7043394.1"
                     /translation="MGVSLRPSRRTNLALLVSLAGAFVTGWVAFGFEGSRESRVVAVV
                     HATLGFGLLVLAPWKTVIVRRGLRRGRRHGLAVAFGVVVGVSLVAGVVHATLGPLEVA
                     GITAIQVHVGLAVAAVPLAALHVVRRPQRPRATDLSRRTVVRAIGVGAGATLVYGAVE
                     SFTSLAGLPGARRRATGSYQIGSFVPSAMPVTQWFTDGVPRIDVAAYELLVSGAEEPS
                     LRLSYDELLSMPASTKAAVLDCTGGWWAEQTWRGIRLDALLGTPENGSVAVRSATGYT
                     RRFAPEAVSRLLLATHVGGDPL"
BASE COUNT          804 a         1582 c         1513 g          801 t
ORIGIN      
        1 gcagaatcaa tggatcagtt cgctcgacgt cgtcgcgcac gagcacggcc acggcgtcga
       61 cgaccacacg ccgggcggca tctcggggaa cggcacccag gagttcgtcg ccgacacctt
      121 cggcgccgcg acggagtggt acgccaacga gccttcctcc tacgacccgc ccgacttcac
      181 cgtcggtgag gaggtcaacc tggtcggctc cggcccgatc cgctacatgt acaacccgtc
      241 ccttgccggt gacgacaact gctactcgtc gtccatcccg aacgaggagg tccatgcggc
      301 agcaggtccg ggcaaccact ggttctacct gctggccgag ggcagcagcc cgaccaacgg
      361 ccagccgacc agcccgacct gcaacggctc gtcagtgacc ggcgtcggca tccagaaggc
      421 cgagcagatc atgtacaacg cgatgctgat gaagaccagc tcgtcgtcgt acctgaagta
      481 ccgcacctgg acgttgaccg cggcgaagaa cctcgacgcg acctgcggtc tgttcaacgc
      541 caccaaggcg gcgtggaacg ccgtgtcggt cccggcccag tcgggcgacc cgacatgcac
      601 cggcggtggc gggaacaaca cggtgacggt gaacaatccc ggcaaccagt cgggcaccgt
      661 gggcacggcg acgtccctgc agatgaccgg caccgactcg cagtccggcc agacgctgac
      721 ctggtctgcc accggtctgc cggcgggcct gtcgatcaac gcctcgaccg gtctgatcag
      781 cggtacgccg acgacggcga acacctacac cccgacggtg acggccacgg acacgaccgg
      841 agcccacggc tccacgtcgt tcacgtggac gatcggcggc ggcggtggcg gtggctgctc
      901 gagccccgga cagaagctcg gcaacccggg cttcgagacg gggtcggcgt cgccgtggtc
      961 cgcgtccgcg ggcgtcatcg acagctcgag cgcggagccg gcccactcgg ggtcgtggaa
     1021 ggcgtggctg gacggctacg gccgcaccca caccgacacc ctgtcccagt cggtcaccat
     1081 cccggcgggc tgcgcagcca cactgtcgtt ctggctccac atcgacacct cggagaccac
     1141 cacgacgacg gcgtacgaca agctgacggt caaggccggc agcacgacgt tggcgacgta
     1201 ctcgaacctg aacaagaaca ccgggtacag ccagaagtcc ttcgacctct ccgcgttcgc
     1261 ggggcagacc ctcacgatct cgttcaccgg tgtggaggac gtgagcctcc agacctcgtt
     1321 cgtgatcgac gacaccgctc tcaccgtcag ttgatggcct gacggcgagc cgacgtccgc
     1381 ttggacgtcc cacccggaac gccgggtcga ccacctcacc ggtcggcccg gcgttccacc
     1441 gcgtccgtcg gccgaactag tcaagaccgt ccgaacaggt cctacaatca gcgacgcgga
     1501 ggggctggtc gggcgaccgc gacgtcgcgt cttcgggcgc ggtgtcgagg aaggtccggg
     1561 ctccgcaggg cagggcggtg ggtaacaccc acccggggca acccgcggga aagtgccaca
     1621 gagaacaaac cgccggctcg tgctccgtcg gtcgagctag tcgagaccgt cgggaccacg
     1681 agtcggtaag ggtgaaacgg tggtgtaaga gaccaccagc ggcccgggcg accgggtcgg
     1741 ctcggcaaac cccgcccgga gcaaggtcga acagcgcacg tccgagggcg gcccgcccga
     1801 gtgcgcgggt ggaccgcacg aggccgccgg caacgacggt ccgagatgga tggtcgccga
     1861 acggtgcgcc gcaaggcgcc cgggaacaga acccggccta cagaccagcc cctccgcccc
     1921 atcccatctg acctggggtt ttacgttcag agggcctcta ttgtgcccat cctggtgccc
     1981 acgattttca ctcgcggtca ccgatgcccg ccgatgagtg ctgccgagaa ggctgtccac
     2041 gatggcccgg ctgcgcgtgt cctcgtcgcc catgaggtgg ccttatgacc ccgtcggcag
     2101 gtcgcatccg gctgacggct gaagtctgtt ggcgcctccc cgctcgcgac ctcgtgaggc
     2161 ggccacctcc atcaagagtt aggcacgctg taagtgtcga tctgatcacc actaattgcg
     2221 ttcaagaagg tccaggtggt gcacgacgga gtcgctgcat cctgcggcac cgcgctagag
     2281 gacggcgact gctcagatgg tgaatcagaa ggccccgggc tggcggtatc agctgttggg
     2341 ccgccgatcg gcccgataca gtaatcctcc tggaatgccc agacgggggt gcccgattcc
     2401 ttaacctcta ctccgggctc cccttctggc ccggtaagca aggtaagcct tccatacatc
     2461 gcggatatcg agtcgggaag cggcttaccg tttgaccact tctcccaagc cgcatcggcg
     2521 gataagtacc cggcatcgct tgggaacgtc tcgggcgtga aagtctctcc gacaacaggg
     2581 tctattgtca caactgccga ctgggtggga gctccgccgc ctggggcagc acggtcacgt
     2641 ccaatgtgtt cagtcgccaa cgcaaccgcc gcgattaggg ccgcaactag taccgaaacg
     2701 ccggcagttg ccttcccctt agtcaacatt ccggtcatcc cacaccacca gtgtctgtgg
     2761 atttggtaaa tctttgtgga aatggtcgca ggtataggcc ccatcctgag ggacaccgtc
     2821 gaaggtttcc gtgtcaatcc acggatcacc ggagtcgtgc tccacctcaa ttggcttact
     2881 gctgctccca atattcgcgg ggctagtgga gttgccaaac atggtgtcct ggcagtactt
     2941 gtgtgtctct tcggcagagg ctatcgcgtg gtcgtacaag atgtagttgt cggagatgcc
     3001 ggaaagttcc gtcacacaag ttccgtaggg atctgagtcc cactctccgc aatccagaac
     3061 ctcgaccatc ttccctgacg gagtgttgta cttcttaacg atgaacgagt agtaggcccc
     3121 gccattgaaa tcaccacaat cgtacaggtg tgcgctttgg gctgggtatt tctcgtagaa
     3181 cttgcacatg tgtggggtgc cctggccgtc tcggtcgtgg aataggcccc cttgcaccag
     3241 gccatttccg gacatgttct cttcttccag gccgatccag agtgccaccc cttgaccgtc
     3301 cacagatgcg cacaggtcgc tgtccttgcg aacctggatc cctgcgcgaa caccgccagc
     3361 gttcttgtcc ccagggtaat cttcccaagt cgcgtataag tggttttgag tcccgtctgg
     3421 agtggcatca catgccgctt gggccggagc cgccgagatc ggcaacatca tcgccgcctg
     3481 aacgcaacca gtcctagaag ggccaaccga aatctcacgc gcggaacgta gcatcctgag
     3541 gtctctcttg gccgtccccg tgggtttcct ttcggcaaaa ttcttgcccg caccccaaat
     3601 cggtcgacga tcccgacgga cacgagaacg aggagtcgcg cgctcggcga gccctcctcg
     3661 accagagggc gagctcccac ggcggccgcc gcctgggtct cctcgtagcc acccttcgcg
     3721 ctgaaccacc gctggtactg aaagatgaga ggcgagatca tcgggcacgc gacgtgaaca
     3781 ttggccgagc gctgcacact ggcgtggtgg gcgtctcgct ccgtccgagc cggcggacca
     3841 acctggcgct cctcgtgtca ctggcggggg cgttcgtgac cggctgggtg gccttcgggt
     3901 tcgagggttc gcgggagtct cgggtcgtcg cagtggtcca cgccaccctg ggcttcggcc
     3961 tgctcgtact cgcgccgtgg aagacggtga tcgtgcgacg cggactgcga cggggacggc
     4021 ggcacggtct cgcggtcgcg ttcggcgtgg tcgtcggcgt cagcctggtt gccggggtgg
     4081 tgcacgcgac gctcggcccg ctcgaggtgg ccgggatcac cgcgatccag gtgcacgtcg
     4141 gcctcgccgt cgcggctgtg ccgctggcgg cgctccacgt cgtacgtcga ccgcagcggc
     4201 cccgtgcgac ggacctgtcc cggcgcaccg tggtgcgggc gatcggagtc ggcgctggtg
     4261 cgacgcttgt gtacggcgcg gtggagtcgt tcacgtcgct ggccgggctc ccgggcgcgc
     4321 gccgacgggc gaccgggtcc taccagatcg ggtccttcgt accgtccgcg atgcccgtca
     4381 ctcagtggtt caccgacggc gtaccgcgca tcgacgtcgc ggcgtacgag ctgctggtca
     4441 gcggtgccga ggagccgtcg ctccggcttt cctacgacga gctgctctcg atgccggcct
     4501 cgacgaaggc ggcggtcctg gactgcactg gaggttggtg ggccgagcag acctggcgcg
     4561 gcatccggct ggacgccctg ctcgggaccc cggagaacgg gtcggtcgcg gtgcgttccg
     4621 cgaccggcta caccaggcgc ttcgcacccg aggccgtcag caggctgctg cttgccacgc
     4681 acgtcggcgg cgacccgctc
//
LOCUS       DATBFZ010000232         1791 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_149197, whole genome shotgun sequence.
ACCESSION   DATBFZ010000232 DATBFZ010000000
VERSION     DATBFZ010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149197"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(175..>1791)
                     /locus_tag="VH419_06990"
     CDS             complement(175..>1791)
                     /locus_tag="VH419_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043395.1"
                     /translation="PAGKVSAPTRRATRQLEDHPSMAGKDPDDYRPRRAFDDSEADGG
                     TGTSDTGKRDTDRRDVEPDRSTTILPRVRDDRSTTILPKTSERRRHRDELDDDEADVR
                     PPMGRRTRLALTIGAVAVVVILGLVLGYVALNNGRPATEPSTGVSIDTSSGETPTPTP
                     VALLDDASMINEQMAKQIDPKRTWQVASTQRGLDANSPRAVCFASDPVEGQPDPQQTI
                     QRLISSTGKNAPALLHQAEAYGTPEEAAQAYVAISKALGSCAVVGAWIDSGRTISGLG
                     DQSAGAIVVVTDAGKQTYHSMVISRTGRVVDVVDVAQPGSAADIGDSARALGEAVTVQ
                     CQTSGGACAEKVSVKSGPPPLGGDEPGFLAAGDLPPVGATPTLWVGSTPAPPDADYTG
                     SNCETTDWSKVDATTKTMRTYTLEGNPSHFGVDQIILTMSDDKAAADLVDKVKKDLES
                     CSDRKLTATVSKPEEIKAPGAENNEVQGWSADVSHKTASSTVKYRVGIVSVGPKVVWT
                     LANPTDNLDFSEDQWKTIALRAGQRASQVQ"
BASE COUNT          221 a          616 c          608 g          346 t
ORIGIN      
        1 actaaccgac gaaggcgatg cttccacttc ggcagcgtcg cctccacgat ttctgcggcc
       61 cgcactttgg gtaattcttg acattcgttt tgccggctcg agccggttcc cgcctcaggc
      121 ctgtgccccg tcgcgcgacg aatgtcaaga attacccaaa cgcggctgag cccgctactg
      181 cacctggctg gcgcgctgcc ccgcccgcag cgcgatcgtc ttccactggt cctcgctgaa
      241 gtccaggttg tcggtcgggt tggccagcgt ccagacgacc ttgggcccga ccgagacgat
      301 gccgacccgg tacttcaccg tcgagcttgc ggtcttgtgc gagacgtcgg ccgaccagcc
      361 ctggacctcg ttgttctccg cacccggcgc cttgatctcc tccggcttgg acacggtcgc
      421 ggtgagcttg cggtccgagc agctctcgag atccttcttc accttgtcca cgaggtccgc
      481 cgcggctttg tcgtccgaca tggtcaggat gatctgatcg acgccgaagt ggctcgggtt
      541 gccctccagg gtgtacgtcc gcatggtctt ggtggtggcg tcgaccttgg accagtcggt
      601 cgtctcgcag ttcgagccgg tgtagtcggc gtcgggaggt gcgggggtgc tgcccaccca
      661 cagcgtcggc gtcgcgccca ccggggggag atcaccggcg gcgaggaaac ccggctcgtc
      721 gccgccgagc ggcggcggac ccgacttgac cgagaccttc tccgcgcagg caccaccgct
      781 ggtctggcac tgcacggtca ccgcctcacc gagtgcccgg gcgctgtcgc cgatgtcggc
      841 cgccgatccg ggttgggcca cgtcgacgac gtcgacgacc cgccccgtcc ggctgatcac
      901 catgctgtgg tacgtctgct tccccgcatc ggtgaccacg acgatcgcgc ccgcactctg
      961 gtcaccgagt ccgctgatgg tccggcccga gtcgatccag gcacccacca cggcgcagct
     1021 gccgagcgcc ttggagatcg ccacgtacgc ctgggcggct tcctccggcg tgccgtacgc
     1081 ctccgcctgg tggagcagcg cgggcgcgtt cttcccggtg ctgctgatca gccgttggat
     1141 ggtctgttgt ggatcgggct gcccctcgac cggatctgag gcgaagcaga cggcgcgcgg
     1201 agagttggcg tccaggccgc gttgggtcga ggcaacctgc caggtgcgtt tcgggtcgat
     1261 ctgcttcgcc atctgctcgt tgatcatgga cgcgtcgtcg agcagtgcga ccggtgtggg
     1321 ggtgggtgtc tccccgctgg aggtgtcgat cgagacgccc gtcgagggtt cggtggcggg
     1381 gcggccgttg ttcagcgcga cgtagcccag cacgaggccg aggatgacca ccacggcgac
     1441 ggcaccgatg gtcagggcga gccgggtgcg cctccccatc ggtggacgta cgtcggcttc
     1501 gtcgtcgtcg agctcgtcgc ggtgccggcg ccgttcgctc gtcttcggca agatcgtggt
     1561 gctccggtcg tcgcgaaccc tgggcaggat cgtggtcgat cgatccggct cgacgtcgcg
     1621 ccggtcggtg tccctcttac cggtgtcgct cgtgccggta ccaccgtcgg cctcggagtc
     1681 gtcgaacgcg cgtcgcggcc gatagtcgtc cgggtcctta cctgccatgc tcgggtggtc
     1741 ctccagctgt cgcgtcgccc gtctggtcgg cgcagacacc ttgcccgccg g
//
LOCUS       DATBFZ010000233         2522 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_2446437, whole genome shotgun sequence.
ACCESSION   DATBFZ010000233 DATBFZ010000000
VERSION     DATBFZ010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2522
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2446437"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /locus_tag="VH419_06995"
     CDS             complement(<1..529)
                     /locus_tag="VH419_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043396.1"
                     /translation="MGQKRTRLPLALALLATASIVATTLEPASAGAVARTPGPSAAAP
                     VPSHHAPTSALRRAGGVVAPIPVLHWHACQWDASLECTRATVPLDYDHPNGATTRLFM
                     VKRPASPLPGQQTIGTLFVNPGGPGGPSSEVADIFARLLGKPVQRHFDVIGIDPRGVG
                     GSTPVRCRIPGPTPAF"
     gene            complement(702..1502)
                     /locus_tag="VH419_07000"
     CDS             complement(702..1502)
                     /locus_tag="VH419_07000"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013866136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HEY7043397.1"
                     /translation="MRPLHIGGHVDQVDPIAEARDRKATLSQFFLGDPQSYRGPVVSY
                     AGGAAGLKADAEDAGIDLYVHAPYPINVASTNNRIRIPGRKLLQQHLKAAAEIGAKGV
                     IVHGGHVLNEDDPEVGFDNWRKCVDGLEMPVPLLIENTAGGDNAMARRLERIDRLWDA
                     LAKAKGGDTVGFVLDTCHAHAGGEELATVVDRVRTITGRIDLVHANDSRDDFDSGADR
                     HTNLGSGRIAADDLVTVVREAAAPVVCETPGGVDGQSADIAWLRDHLD"
     gene            1750..>2522
                     /locus_tag="VH419_07005"
     CDS             1750..>2522
                     /locus_tag="VH419_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012923273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY7043398.1"
                     /translation="MSDVNAGQATGSQSESNQTVAGQAASTGQPAGQNGIAARSVDLH
                     KVYGEGDAAVAALAGVSVEFYTGEFTAVMGPSGSGKSTLMHCMAALDDATSGQVFLGA
                     EELTRMGDKLLTRVRRDRIGFVFQAYNLVPTLTAEENILLPMSIAGRRPDKQWFDQVI
                     GTVGLGDRLHHRPSELSGGQQQRVACARALMSRPEVVFADEPTGNLDSTSGAEVLGFL
                     RRCVDDYHQTIVMVTHDPVAASYANRVLFLSDGHIVSQL"
BASE COUNT          404 a          848 c          884 g          386 t
ORIGIN      
        1 ggaacgccgg agtcgggccc gggatgcggc agcgtaccgg ggtacttccg ccaacgccgc
       61 gcgggtcgat cccgatcacg tcgaagtggc gttgcaccgg cttccccaac aacctggcga
      121 agatgtcggc cacctcggag gacggaccac cgggtccgcc agggttgacg aacaacgtcc
      181 cgatggtctg ctggccgggc agcgggctcg ccggccgctt gaccatgaag agccgagtcg
      241 tcgcgccatt gggatggtcg tagtccagtg gaaccgtcgc tcgggtgcac tcgagcgagg
      301 catcccactg gcaggcatgc cagtgcagca cgggaatggg agccaccacg ccaccagcac
      361 gtcgcaatgc cgatgtcggt gcgtgatggg acggcaccgg ggcagccgcc gacggcccgg
      421 gtgttcgcgc cacggcccct gccgacgccg gctcgagcgt cgtggcgacg atgctcgcgg
      481 tggccaacag cgcgagcgcg agtggcaggc gagttcgctt ctgtcccatc gtgtcccccc
      541 gtccacgagc acccgccgcc cgaccagcaa ctggtgctga gtcctgcgaa ggtacacccg
      601 accgcaaccg ttcgacctcc gcgaattgcg ggaaaggtgt tgcccacacg tccccgacga
      661 catgactttt cccgcaattc gcgggagccc gcggaggcgg gtcagtcgag gtggtcgcgc
      721 agccaggcga tgtcggcgct ctggccgtcg acgccgccag gcgtctcgca cacgacgggc
      781 gcggccgcct cgcggaccac cgtgacgaga tcgtcggcgg cgatacggcc ggaaccgagg
      841 ttggtatgcc ggtcggcgcc tgagtcgaag tcgtctcgcg agtcgttggc gtgcacgagg
      901 tcgatccggc cggtgatggt gcgcacgcga tcgacaacgg tggccagctc ttcgccgccg
      961 gcatgggcgt ggcaggtgtc gagcacgaag ccgacggtgt cgccgccctt cgccttggcg
     1021 agcgcgtccc agagacgatc gatgcgctcg agccgacggg ccatcgcgtt gtcgccgccc
     1081 gcggtgttct cgatcagcag tggcaccggc atctcgaggc cgtcgacgca cttgcgccag
     1141 ttgtcgaagc cgacctcggg atcgtcctca ttcaggacgt gaccgccatg gacgatgacc
     1201 cccttggcgc cgatctcggc agcagccttc aggtgctgct gcaacagctt gcgaccaggg
     1261 atacggatgc ggttgttggt ggacgcgacg ttgatcgggt agggcgcgtg cacatacagg
     1321 tcgatccctg cgtcttcggc gtcggccttg agcccggcgg ccccaccggc gtacgacacg
     1381 acaggccccc tgtagctctg cgggtcaccg aggaagaact gcgacaacgt ggccttgcgg
     1441 tccctggcct ccgcgatcgg gtcgacctga tcgacatgtc ctccgatgtg caacgggcgc
     1501 atgcagccac tgtatgacgc gtcgccgaca gcggacgcgg ccgcgagcgc accagggaca
     1561 catgcgccac ccacgacgaa cgtcacggcc gatcagggac gtctggcatg cgtcgtgggg
     1621 gtgcggccag gggacgatca ggggaaaccc gatcgtccgg ggtgacgaac ggccgctagc
     1681 gtggacgcgt cgcactgctc gcacccggac cagccaggca gggccggcca cgattgttcg
     1741 aggggatcga tgtcagacgt caatgccggt caggcgaccg gttcgcagtc cgagtccaac
     1801 cagaccgtcg ctgggcaggc agcgtcgaca ggccagccgg ccggtcagaa cggcatcgcc
     1861 gcgcgctcgg tggacctgca caaggtctat ggcgagggtg acgcggccgt tgcagcactg
     1921 gccggggtga gcgtggagtt ctacaccggg gagttcacgg cggtcatggg accgtccggt
     1981 tcgggaaagt cgacgctcat gcactgcatg gcggcgctcg acgacgccac gtccgggcag
     2041 gtcttcctcg gcgccgagga gctgacccgg atgggcgaca agctgctgac gagggtccgt
     2101 cgcgaccgga tcgggttcgt gttccaggcg tacaacctgg tgccgaccct cacggcggag
     2161 gagaacatcc tcctgccgat gtcgatcgcc gggcgtcgtc cggacaagca gtggttcgac
     2221 caggtgatcg gcaccgtcgg gctcggggat cgacttcacc accggcccag cgagctgtcc
     2281 ggcggccagc agcaacgcgt cgcgtgcgcc cgcgctctga tgtcgcgccc ggaggtcgtg
     2341 ttcgccgacg agccgaccgg caacctcgac tccacctccg gagcggaggt cctgggcttc
     2401 ctccggcggt gtgtcgacga ctaccaccag acgatcgtga tggtgacgca cgacccggtg
     2461 gccgcgtcct acgccaaccg cgtcctgttc ctctccgacg ggcacatcgt gagccagctc
     2521 tt
//
LOCUS       DATBFZ010000234         5329 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_2446732, whole genome shotgun sequence.
ACCESSION   DATBFZ010000234 DATBFZ010000000
VERSION     DATBFZ010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 5329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5329
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2446732"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..770
                     /locus_tag="VH419_07010"
     CDS             <1..770
                     /locus_tag="VH419_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017936242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HEY7043399.1"
                     /translation="VLESGIDAGRYVGGHPMAGSERSGPLASAADLFDGRAWAVVPHS
                     ASTPQAVAVVRWLAHTCGAVSTEMDAADHDLAVARISHLPHLLAALTAGQLGQAPAAH
                     LALAGQGVRDVTRIAAGDPALWRQIAAANAGPLTDLLLDVRSRIDELVGSLAHGDEGG
                     LETVLAAGVAGTAAIPGKHGGPSIELAEVTVAIPDEPGALARLFTDAGDAGVNIEDIR
                     IDHDPARAYGLVELDVMADVADRLVTALGARGWAAHR"
     gene            782..1492
                     /gene="cmk"
                     /locus_tag="VH419_07015"
     CDS             782..1492
                     /gene="cmk"
                     /locus_tag="VH419_07015"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020577022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEY7043400.1"
                     /translation="MSGTTTIERLVIAMDGTAGSGKSSASRGVAQALGLRYLDTGAMY
                     RAMTWQMLLDGVDVYDADAVAARADTTAIDAGTDPVVPTITLHGTDVSREIRSGEVNV
                     AVSPVSAVPHVRELLVAQQRRIIGSGGIVVEGRDIGTVVAPEAPVKVFLSAHPAARAQ
                     RRAAELTGSHRHDVDAVEQALVRRDAYDSSRATAPLTVADDAVQIDTTDLRLDEVVAR
                     IVALVRERVDTDAAGMVR"
     gene            1489..2841
                     /gene="der"
                     /locus_tag="VH419_07020"
     CDS             1489..2841
                     /gene="der"
                     /locus_tag="VH419_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020384860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEY7043401.1"
                     /translation="MTEAIEPTEVTGPLPVLAVVGRPNVGKSTLVNRILGRREAVVED
                     TPGVTRDRVTYDALWNGRRFTIVDTGGWDPDARGMAEQVSLQAEVAVSLADAVLFVVD
                     AGVGITDTDETVVKILRRSGKPVVLAANKVDDQRAEADAALLWNLGLGEPYAVSALHG
                     RGSGDLLDAVLEALPQAPAEVFDAPRGPRRVAIVGKPNVGKSSLLNKLAGETRVVVDD
                     VAGTTVDPVDEIISLGGRTWQFIDTAGIRRRVREASGHEYYASLRTASAMERAEVAVV
                     VIDASEPLTEQDQRIISSVEESGRAVVIAFNKWDRVDEERRYYLDREIERELVRVQWA
                     PRMNVTALTGWHVDRLVPALDRALGGWETRVPTAALNAFLGRVVAAHPHPVRGGKQPR
                     VLFATQASIAPPRFILFTSGLLEAAYLRFVERRLREEFGFVGTPIHLTVKPRAKRKRS
                     "
     gene            2851..3690
                     /locus_tag="VH419_07025"
     CDS             2851..3690
                     /locus_tag="VH419_07025"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017934507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY7043402.1"
                     /translation="MTGPLIGVTGSTGQVGGRVAAALAEQGRSMRLLVRNPAKAPVYD
                     GVEVRQAEYADHGASLDALRGVEVLFMVSAAENERRLEEHATFVDAAAEAGVRHVVYT
                     SFYGASPDCTFTLGRDHYATEERIRASGMEWTFLRDNLYLDYVPLFAGDEGVLRGPAR
                     DGRVSAVSQDDVAASAVGVLQNVDSHRGAVYGLTGPEAFTLAEAAETMSRVTGRECVF
                     VNETVDEAYASRAPYGAPQWQLDAWVSTYVAIAVGEMDGVTDVVELLSGRPPISLEEL
                     LRR"
     gene            complement(3701..5107)
                     /locus_tag="VH419_07030"
     CDS             complement(3701..5107)
                     /locus_tag="VH419_07030"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HEY7043403.1"
                     /translation="MSESLSPSQRARRLRRAAGVPRQAGPRPDSRKVQEWAAHQRTMQ
                     AKLARIGKIERKIAAMQAEQVALTAAFVDERIAFDEAHGFLADQAQYRGMLAEVAIAR
                     RVSVTTAASFMDEAWQLAHDHPATMAALQTGRIGLSAARSIVKETAVLNDPAAKALAD
                     QVIAEEAPDVLPGKVRALAERRVAEIDPDAVNRRSERERKDRHVGLAMAGSGMAWLSS
                     YLPAEQAKAAYDSVHEHARSVYAAGDDGRTVSQIMADTLVERLTGSATDALPVDINVV
                     MTDRTLLGLSDQPAHLVGGGPLPSPTARLLAGSTNAWLRRFLTDPVDGSISHGDSRRR
                     RFDGRLRDLQVVRDQHCRGIQCGSRIVDIDHIHDHAKGGETTPSNGQGLSKACHISRD
                     DPRMHVERDEITGVVTWTTPTGFTWRSLPPPSLAPGSLSAKQRRWRSWLLHPPESRLE
                     QQTIHVTVEHLKHSHQHC"
BASE COUNT          757 a         1700 c         2001 g          871 t
ORIGIN      
        1 acgtgctgga gtccgggatc gacgcggggc ggtacgtcgg tggtcacccg atggctggca
       61 gtgaacggtc cggaccgctg gcctcggcag cggacctgtt cgacgggcga gcctgggccg
      121 tcgtaccgca ctcggcgtcg accccgcagg cggtcgccgt ggtccgctgg cttgcgcaca
      181 cgtgtggagc cgtgtcgacg gagatggacg cggcggacca cgacctcgcg gtggcccgga
      241 tctcacacct gccgcacctg ctggccgcgc tcaccgcagg tcagctcggc caggcgccgg
      301 ccgcgcatct ggcgctcgcc ggtcaggggg tccgcgatgt cacgagaatc gccgccggcg
      361 acccggcgct gtggcggcag atcgcggccg cgaacgcagg tccgctgacc gacctgctcc
      421 tcgacgtgcg gtcgcggatc gacgagctgg tgggttccct cgcccacggc gacgagggtg
      481 ggctcgagac cgtgcttgcg gcgggcgtcg ccgggaccgc ggccattccc ggcaagcacg
      541 gcggtccgag catcgaactg gccgaggtga cggtggcgat cccggacgaa cccggggcgt
      601 tggcgcggct gttcaccgat gccggcgacg cgggtgtcaa catcgaggac atccggatcg
      661 accacgaccc tgcgcgggcg tacggtctcg tcgagctcga cgtgatggca gacgtcgcgg
      721 atcggctcgt gacggcgctc ggtgcacgcg gctgggcggc gcaccggtag cctcggtcgc
      781 tgtgagcggg acaaccacga tcgagcgact cgtgatcgcc atggacggca cggccgggtc
      841 aggcaagtcg agcgccagcc gcggagtggc tcaggcgctg ggactgcgct atctcgacac
      901 cggtgcgatg taccgggcga tgacgtggca gatgctgctc gacggcgtgg acgtgtacga
      961 cgccgacgcg gtggccgctc gggccgacac gaccgcgatc gacgcgggca ccgacccggt
     1021 ggtcccgacg atcacgttgc atggcaccga cgtgtcccgc gagatccgca gtggtgaggt
     1081 caacgtggcc gtgagcccgg tcagcgcggt cccgcacgtc cgcgagctgc tcgtcgcaca
     1141 gcaacgtcgg atcatcggct cggggggcat cgtcgtcgag gggcgggaca tcggcacggt
     1201 ggtggcaccg gaggccccgg tgaaggtgtt cttgagtgcg cacccggccg cgcgggcgca
     1261 acgacgggcg gccgagctca ccggctcgca tcgtcacgac gtggacgctg tggagcaggc
     1321 cttggtccgt cgggacgcgt acgactcatc gcgggcgaca gcaccgctca ccgtggccga
     1381 cgacgcggtt cagatcgaca ccaccgacct tcgattggac gaggtcgtcg cccgcatcgt
     1441 cgcgttggtg cgcgagcgcg tcgacaccga cgccgcggga atggtgcgat gaccgaggcc
     1501 atcgaaccca cggaggtgac cgggccgctg ccggtgctcg ctgtcgtcgg acggccgaac
     1561 gtcgggaagt ccacgctggt gaaccgcatc ctcggtcgcc gggaggccgt ggtggaggac
     1621 acgcccggcg tgacgcggga ccgggtgacg tacgacgcgt tgtggaacgg acgccggttc
     1681 accatcgtcg acaccggcgg ctgggatccc gacgcccgcg gaatggcaga gcaggtctcc
     1741 ctgcaggccg aggtcgcggt gagcctggcg gatgcggtgc tgttcgtcgt ggacgccggc
     1801 gtgggaatca ccgacaccga cgagaccgtc gtgaagatcc tcagacgctc gggcaagccc
     1861 gtggtactgg cggcgaacaa ggttgacgac cagcgcgccg aggccgacgc ggccctgctc
     1921 tggaacctgg ggctgggcga gccgtacgcc gtctcggccc tgcacggacg cgggagtggc
     1981 gacctgctcg atgcggtgct cgaggccctt ccccaagcgc cggcggaggt cttcgacgcg
     2041 ccgcgcggac ctcgccgggt ggcgatcgtc ggcaagccga acgtcggaaa atccagcctg
     2101 ctcaacaagc tcgccggtga aacgcgggtc gtcgtagacg acgtggccgg cacgacggtc
     2161 gacccggtcg acgagatcat ctcgctgggg ggccgtacct ggcagttcat cgacactgcc
     2221 gggatccggc gtcgggtacg tgaggcgagc gggcacgagt actacgccag tcttcgtacc
     2281 gccagcgcga tggaacgcgc cgaggtcgcg gttgtggtga tcgacgccag cgagccgctg
     2341 accgagcagg accagcgcat catctcaagc gtggaggagt ccggccgagc ggtcgtcatc
     2401 gccttcaaca agtgggacag ggtcgacgag gagcgtcgct actacctcga tcgtgaaatc
     2461 gaacgtgagc tcgtgcgggt gcagtgggcg ccgcgcatga acgtgacggc gctgaccggt
     2521 tggcatgtcg accggctggt cccggccctc gaccgggccc tcggaggctg ggagacccgg
     2581 gtgccgactg ctgcgttgaa tgcgtttctc ggtcgcgtgg tcgctgcaca cccacatccg
     2641 gtccgtggcg gcaagcagcc gcgagtgctg ttcgcgaccc aggcgagcat tgctccgccg
     2701 cgcttcatct tgttcacgtc tggcctgctc gaagcggcgt acctgcgttt cgtcgaacgg
     2761 cggctgcgtg aggagttcgg gtttgtcggc acgccgatcc acctgacggt caagccgcgg
     2821 gcgaagcgga aacgctcgta gggtgagggc atgactggtc ccctcatcgg cgtcactggt
     2881 tcgaccggcc aggttggcgg ccgggtcgcc gcagcgctcg ccgagcaggg gcggtcgatg
     2941 cggttgctgg tgcgcaaccc ggcgaaggcg ccggtgtacg acggcgtcga ggttcgccaa
     3001 gcggagtatg ccgaccacgg cgcctcgctg gatgcgttgc gtggggtcga ggtcctgttc
     3061 atggtgtcgg ccgctgagaa cgagcgccgt ctcgaggagc acgccacgtt cgtggacgct
     3121 gctgcggagg ctggcgtgcg gcatgtcgtg tacacatcgt tctacggcgc gtcacctgac
     3181 tgcacgttca cgctcggacg ggaccactac gccacggagg agcggatccg ggcgagtggc
     3241 atggagtgga cgttcctgcg cgacaacctg tacctcgact atgtcccgct gttcgccggc
     3301 gacgagggag tgctccgcgg cccagcccgg gacggaaggg tgtcggcggt ctctcaggac
     3361 gatgtggccg cctcggcggt tggcgtcctg cagaacgtcg actcgcatcg gggagcggtc
     3421 tatggcctga ccggtccgga ggcgttcacg ctcgcggagg cggcggagac gatgtcgcgg
     3481 gtgaccggtc gggagtgcgt tttcgtcaac gagacggtgg atgaggcgta tgcgtcgcgg
     3541 gcgccgtacg gcgcaccaca gtggcagctg gatgcgtggg tcagcaccta cgtcgctatc
     3601 gcggtggggg agatggacgg tgtcacggac gtggtggagc tactctcggg tcggccgccg
     3661 atcagcctgg aggagttgtt gcggcgctga gtcggcgagc tcagcagtgc tggtgggaat
     3721 gtttgaggtg ttcgaccgtc acgtggatcg tctgctgctc gagccgcgac tcgggtgggt
     3781 gcagcagcca ggatcgccat cggcgttgtt tggcgctgag cgagccggga gccaggctcg
     3841 gcggcgggag gctgcgccag gtgaagccgg tcggagtggt ccaggtgacc acgccggtga
     3901 tctcgtcgcg ctcgacgtgc atgcgcgggt cgtcgcggct gatgtggcag gccttcgaga
     3961 gaccctggcc gttggacggc gtggtctcac cgcccttggc gtggtcgtgg atgtggtcga
     4021 tgtcgacgat gcgggacccg cactggatgc ctcggcagtg ttggtcgcgg acaacctgga
     4081 ggtcgcgcag tctcccgtcg aagcgacgtc gtctggagtc gccatggctg atcgatccgt
     4141 cgacgggatc ggtgaggaag cggcgtagcc atgcgttcgt gctcccggcg agcagccggg
     4201 ccgtcggcga cggcagcggg ccgccaccga ccaggtgggc tggctggtct gagagcccga
     4261 gcagggtccg gtcggtcatg accacgttga tgtcgaccgg gagcgcgtcg gtggcgctgc
     4321 cggtgagccg ctcgaccagt gtgtcggcca tgatctggga gaccgtccgg ccgtcgtcgc
     4381 cggcggcata cacgctgcgc gcgtgctcgt gcacgctgtc gtaggcggcc ttcgcctgtt
     4441 cggccggcag atagctgctg agccaggcca tgccggatcc ggccatggcc aggccgacgt
     4501 ggcggtcctt tcgctctcgt tcgctgcgcc ggttgacggc atcgggatcg atctcggcca
     4561 cccgccgctc ggccagcgcc cgcaccttac cgggcagcac gtcgggcgcc tcttcagcga
     4621 tcacctggtc ggccagcgcc ttggcagcag ggtcgttcag gaccgcggtc tcctttacga
     4681 tcgagcgcgc agcggacagg ccgatccggc cggtctgcag cgctgccatg gtggccggat
     4741 ggtcatgcgc cagctgccag gcctcgtcca tgaaactggc ggctgtcgtc accgagacgc
     4801 gtctggcgat cgcgacctcg gcgagcatcc cgcggtactg cgcctggtcg gccaggaagc
     4861 cgtgcgcctc gtcgaaggcg atccgctcgt cgacgaacgc cgcggtcaac gccacctgtt
     4921 cggcctgcat cgccgcgatc ttgcgctcga tcttgccgat cctcgccagc ttcgcctgca
     4981 tcgtccgctg atgagccgcc cactcctgca ctttcctgga gtcagggcgc ggcccggctt
     5041 gtcgcggcac tccggccgca cgacgcagcc gtcgggctcg ctgggagggt gacagggact
     5101 cgctcatgtg ttcgaatata gtcgagactg gcgacagttt tcggcggcgt acacgaatct
     5161 gtggaatgcc gtcggctgct gtggacaacc cgactgtcgc cggcgtttcg cctggtgggg
     5221 agtcgctggg gtacgctgtc gcagccgttt ccgggtggcc accgcgttct cggaaacggg
     5281 ccgcgggctg tggcgcagtt tggtagcgca cttgactggg ggtcaaggg
//
LOCUS       DATBFZ010000235        16845 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_2446781, whole genome shotgun sequence.
ACCESSION   DATBFZ010000235 DATBFZ010000000
VERSION     DATBFZ010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 16845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16845
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2446781"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(131..2794)
                     /locus_tag="VH419_07035"
     CDS             complement(131..2794)
                     /locus_tag="VH419_07035"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY7043404.1"
                     /translation="MATAQTNKQTTHRGEHNESGLPPYVSEAFLAEGSVAEVRSVEPS
                     DKEGLLRLHAQASDESIRRRFFALNRDQAARYVEHVCSGESDTISLVAVLNGQIVGLV
                     TAEGDGSDSAEVALFVDESKHGFGIGTLMLEHLAAWTYGHGIDVFHAEVLSDNLPMLR
                     VFRDAGFDLEQHQDHGVVSVTLDVRPTRRSQAATDRRERHAERASLRPLFEPSAVAVI
                     GVSRRRGGIGREIVENIRSENFEGRLLAVGHADLTIDGVEALADVDELPIGLDLAVVT
                     VPAAYVLDIVERVAIRGTRVCVIVTSGLAETGESGREAEREVAAVARRAGMRVVGPNC
                     FGVLSRLRDTRLNATFGTARPGAGGLAIGSQSGGVGIALMDAASRRNLGLAAFVSLGN
                     KLDVSGNDLLAAWEEDPDIRVGALYLESFGNPSKFVRLASSFGHAKPLLAVFGGTSAA
                     GRRGGASHTAASATPRRALDAVFRAAGVIALDGVADLVDTAALLDEQPLPDGSRIGLV
                     SNAGGLGVLAADATQNSSLTVPALSEALQTELADLGSGVAGTSNPVDLGAGASSDRFA
                     GAVSVLLGSTEVDALLVVVAATAVSDLGDVVAAVDAAVTAHHTKPCALVVIGSEVTAS
                     HGGATPFESMDSAVRALHHAAAYSAWRRTAEVPVDEPVRVPGTWVPPATPGWMETPDA
                     AGLLADYGMPTVRFEVVSSPADAVRAADGMGYPVVAKTGRPDVVHKIERSLVHVQLRD
                     GGAVAGAVESIQAECPGPVLVQQQLAGPELALGIVRDAGFGPLVMVATGGTNLALWND
                     PTFLLPPFSSHEALVALQALRTWPLLTGFRGAQQYDVDGLARALQQIGQLAIDWPQVA
                     ELDLNPVIVTASGPVCVDAKVRWSPEPGAID"
     gene            complement(2877..3464)
                     /locus_tag="VH419_07040"
     CDS             complement(2877..3464)
                     /locus_tag="VH419_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043405.1"
                     /translation="MNTIKDTSTLEKGAGGAVEVSSSRRAAHIAMGVLRLVLGWTFLW
                     AFFDKLLALGYSTGRDPETGNVDRFGDAAWIHGGSPTLGFLKFGADGPFEDFYHSIAG
                     DTWADWAFMFGLAAIGISLTLGIFMRLGTFAGVLMYLLMWTVVLPPENNPITDDHILG
                     AAAVLVVGLYYAGRYLGLGRWWEGLAITQKYPILK"
     gene            complement(3593..4147)
                     /locus_tag="VH419_07045"
     CDS             complement(3593..4147)
                     /locus_tag="VH419_07045"
                     /inference="COORDINATES: protein motif:HMM:NF012481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HEY7043406.1"
                     /translation="MRGVVIYESMYGNTHRVANAIAAGLAQACEVSVAAVGELDVHMV
                     RMVRGADLIVVGGPTHVHGVSRRRTRDAAVQAAQEPDSDLALEPGASSDGLREWFEST
                     GSLSVAAAAFDTRTDMPALITGRASHGLAKMLGKHGCRLLVKPESFLVDRENHLVAGE
                     EERARQWGVELARQLTGSSRLASE"
     gene            complement(4215..4508)
                     /locus_tag="VH419_07050"
     CDS             complement(4215..4508)
                     /locus_tag="VH419_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012918377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4235 domain-containing protein"
                     /protein_id="HEY7043407.1"
                     /translation="MAREDRRSNKAAKIAYRPVGLVSGLIGGALAGIIFKQIWRHVRG
                     DDEAPGALESEYDFREILIAAAVQGAIFAVIKAAVDRGGARAFERWTGEWPGD"
     gene            4888..5271
                     /locus_tag="VH419_07055"
     CDS             4888..5271
                     /locus_tag="VH419_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043408.1"
                     /translation="MESEILVELFEADADPEQLDDETRRLRRDLLELDVDSVVPASAG
                     EAPPGTRAVDLAAIGALVVTMRGGVDLVTKVVSVVQSWLHGGARPDRTLKMTVGDNTI
                     ELTDPTDEQQQLLIDKFVQQVAAEA"
     gene            complement(5268..7343)
                     /locus_tag="VH419_07060"
     CDS             complement(5268..7343)
                     /locus_tag="VH419_07060"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HEY7043409.1"
                     /translation="MDGARNALIVANYEYADRGLTRLRAPAKDAEELAEVLGHPDIGA
                     FDVRTVINKPAHIVLREVATFFNDRRPDDLLLLHFSCHGVKDDSGELYLATADTRLDL
                     LEATSVASDFVCRSMDHTRSRRVVLLLDCCYAGAFDRRIRTRGGTGVDINERLGGRGR
                     AVITASTAMEVAIDETGVFPIVDESAALPDGSALEALEDDTQPSVFTGALVEGLRTGD
                     ADRDQDGMVSLDELYDYVYERVRERTQHQTPGKWGDFQGTLYIARRCTPVDTPSELPD
                     SVREALENPLSSVRVAVVPELARMLNGEHQGLALAAREALTSLVATDDSKTVVAAASA
                     ALGTEPPPPPPPPPKPPPKPPPSGWRAWLRRNHRAVLASLAAVLLVVVIAGAVALWPE
                     STTGAIPEEDLVVAFQTDDGVRLSRVDTTNGDVDVITSVDAGNQLLYPVVSPDRRQIL
                     YLTDSPTDDHSFGKVHIMNSDGSDDRELFGDQQCEYSSRPAFSNDGKRLAVVCMGSDG
                     RSTGLYVADADGRNLESVLDSDALSRAPTWQRNQWIIYSELETDEPTGPAVLNRVHPD
                     GSGDTPITAVNEDGQGGASFSDLDWSSSGGLLYIKRGLSLNASNHLKPGYVYTLADGS
                     DQPVQEGPDLAASATWSPGGQKYVEVTAQGGGLVVRTPGSDSATTIDIDPSTGLQDAR
                     PNTPAWGSR"
     gene            complement(7513..8547)
                     /locus_tag="VH419_07065"
     CDS             complement(7513..8547)
                     /locus_tag="VH419_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012918854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HEY7043410.1"
                     /translation="MTESEADVRSDATPGVLAIDAGNSKTDLAIISVDGTVLGTTRGE
                     GFVPHLIGAEDAVTGLVPLVSKAVAEAGLEFEKGTPFVQHVSACLANADLPIEQRLLK
                     EAIDAERWGESSAVVNDTFALLRAGVEVPRGIAVICGAGINCTGMLPDGRTARFAAVG
                     RISGDWGGGGYLWQEAMWWAARAEDGRGEDTRLRTALPDMFGLRSMQALIEAIHLEVI
                     PAERCLDMTPLLFEVAESGDPIAVEIVRRQAGEIVALVVAALRRLEVADEPIDVLLGG
                     GVLTAGHPLLMQTIDSLLAAVAPYAVTRLVDTPPVIGAGLLGLDHIGATSSAQSVLRT
                     ADLSAEPSDP"
     gene            complement(8552..9829)
                     /locus_tag="VH419_07070"
     CDS             complement(8552..9829)
                     /locus_tag="VH419_07070"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012918855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEY7043411.1"
                     /translation="MKLAVVGGGSTYTPELVDGLARRADTLPVEELVLVDPALDRLEI
                     VGDFARRILARQGHGAHLTWTSSLEAGTADADAVLLQLRVGGQQARNEDETWPLECGS
                     IGQETTGAGGLAKAIRTVPVVLQIAEQVRRSNPNAWIVDFTNPVGIVTRSLLQAGHRA
                     IGLCNVAIGFQRRFAGMLGIAPDEVNLDHVGLNHLTWERAVRVDGEDMLPMLLADHAA
                     ELAADIQLPADVLTQLGVVPSYYLRYFYAHDEVVREMLTRPTRASQVAAIERELLELY
                     ADPGLDEKPAQLAERGGAFYSEAAVDLLAPLLAPLPTGEDAAGAPVQVVNVRNAGTLP
                     FLPEDAVVEVPARIDRAGAHPLPVEPLEPLFAGLVAAVTAYERLALDAALLGGRERVF
                     QALLAHPLVGQTDKASALTDRLIAHNRRYLSWA"
     gene            complement(9826..10734)
                     /locus_tag="VH419_07075"
     CDS             complement(9826..10734)
                     /locus_tag="VH419_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020386920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEY7043412.1"
                     /translation="MAANTTVASDLRSAAALPTELPPPRTQRRTPTLEWIALHAVGVA
                     LALLFTLPFVFIALTSLMTDQQTLTRNLWPEPWAWHNLVDTWQTPGFATWWRNTIVYA
                     VSGTVLTILSSFPVAYALARFRFRGRSLCMGIVIATMMLPPQVVIVPMYLVWAKQFHL
                     SGTLWPLIIPMAFGDAFSIFLLRQFLLTIPSEYIDAAKVDGCGDFRAMLRVVVPMSKP
                     AIAAVALFQFFYCWNDYFGPQIYASENPGAWTLSYGLESFKSAHHTNWNLTMAATLLV
                     MAPVIIVFFFAQKAFIEGVTLTGVKG"
     gene            complement(10734..11675)
                     /locus_tag="VH419_07080"
     CDS             complement(10734..11675)
                     /locus_tag="VH419_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020386921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEY7043413.1"
                     /translation="MTRPVTLRTKRRRDFVRNVAFLSPWLLGTGLFFIYPLVSTVYFS
                     FTKYDGFTPPVYNGLDNWRYVFQHYPFFWPALRNTLWLVIVMVTLRVIFGLAIGTLVI
                     KIKTGAGLLRTLFYVPYLAPPVAATMAFAFLLNPGTGPVNVILGKLGLPEPGWFNDPT
                     WSKPALTLLALWGIGDLMVIFMAALLDVPKQQYEAAELDGAGAWKQFRYITLPNIAPI
                     VLFAVVTGIIYTMQYYTQPLVAGKVASGVIGGSGQQFEPGYPEKSTLTLPQLVYNLGF
                     QRFDTGSACVVALVLFAMSMVFTAFLMRRNSGLLGDI"
     gene            complement(11717..13060)
                     /locus_tag="VH419_07085"
     CDS             complement(11717..13060)
                     /locus_tag="VH419_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020386922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY7043414.1"
                     /translation="MARMHKVQRAAVGTAAAALLALVGTACTGSSSGGANDNPNEETT
                     ITFWHGWSAPSEVKAIQENVDRFEAKFPNIHVKVVGNITDDKINQALRAGGDNAPDVV
                     SSFTTTNVGKFCASHVFTDLNPFMEKGGIDPASTFPKPMLEYTQYQGDQCSLPLLGDA
                     YGLYYNKDMFAAAGIKQPPKTLSEFEADAVKLTKSDGDSYSQLGFMPNYHGYETTTDH
                     YAAMWNPTYFDDSGKSTVNTDPAIKDMFEWQKHMVDTLGGFDKLEKYRTTFGDEFGAK
                     NPFQTGQVAMALDGEWRAGFIDDANVDFQYGVAPFPVPDSQADTYGKGYLSGTIIGIA
                     SISDKQNAAWEFVKYLTTDTQAVVSFANAIHNVPSTMDALASSDLDKSPAMKTFIEIA
                     QNPHSSTTPASPDGGTYTQTIQDLGYSYESGKVTDLQAGLDKTADQIDTDIEQAQ"
     gene            complement(13084..14286)
                     /locus_tag="VH419_07090"
     CDS             complement(13084..14286)
                     /locus_tag="VH419_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012918856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEY7043415.1"
                     /translation="MTAQAGTPRLLRGINDRAALDLLLEHGPLSRSQLGALTGLSKPT
                     ASQLLARLERVGLVMHTGSSVGRPGPKAQLYEINPDTAYVAALDVTPGRILSAVADVT
                     GQVVGEHELRTPRRAGADTVERVCSAVDGALRQAGVERSRLHRVVIGTPGAFDPRTQR
                     LRYARHLPGWHDPQILERLADTLGLPVEVDNDVNLAAVAEQHSGRARGHPNFVLLWAD
                     EGLGAALVIDGVLHRGASGGAGEVGFLPLPETPLVRDVGRNNAGGFQQLAGGKQVLEL
                     ARELGFRAGTPEAAVARALSTPGAGDRLIESLAHRVALGLAAIVSVLDPELVVLTGNV
                     LKAGGSRLLAEVRTEVAALAVQRPTIELSGIPEKPVLCGALHVALVRTRDEVFDTIQS
                     TPAVSSTP"
     gene            complement(14636..16450)
                     /locus_tag="VH419_07095"
     CDS             complement(14636..16450)
                     /locus_tag="VH419_07095"
                     /inference="COORDINATES: protein motif:HMM:NF012961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HEY7043416.1"
                     /translation="MDQLGYLPDEAKRARLMVAHRISGERFRVVDRHGMVVFRGRVPA
                     DPVGRWNDNYRVVYRLRFDGLTEPGRYHLVTRGPVVVSSPPFGIVSATRLYGRILHDG
                     VKFDQVQRDGLHVIPGALNRKPAHRHDRRAFVYAKPHFVPGSDLITDRDLHRIGGPVD
                     VSGGWYDAGDYLKFAHSTAYNDVLLFTSAQLVRGRLQELLIAEARHGIQWLDKMWRPR
                     DGVLYMQVGVGSGNRAGTFRGDHDGWRLPQADDHNTRHIDRYVSHRPVFRAAPPHKRI
                     SPNLVGRVSAAFALAAVADARDHPRRARHELTKAKLLFARAATRHPPKQLVTALPHAF
                     YPEDSWQDDMELGAAEIALASRRLGEHARPYVVASGHWARAYIRSRYGDTLNLYDTSA
                     LAHAALVNALSRTGMSGLAVSRADLMRDIQHQLETGVSRSRRDPFASGVIYDNFDANS
                     HAFALVATAAIYHRLSGPHHSSYDRFATSWRNWLLGENPWGVAAMVGIGQTFPHCMQH
                     QIANLEGSTDGSRPLDVGAVVNGPNDARIFAGGLGGYQDGMVHCPGTADPFKPFNGRG
                     SRYVDDVRSWQTDEPALDMTGAAIIAAAEQLAAHRASR"
BASE COUNT         2789 a         5627 c         5701 g         2728 t
ORIGIN      
        1 gggtttgcga tgtgaacatc gcaaacccac gccggccatc aatcgctcag ccgctcgccc
       61 agccgctcga tcagccggtc gctcagccag tcgctcagcc agtcgctcag ccagtcgctc
      121 agccagtcga tcagtcgatg gcgccgggct cgggtgacca gcggaccttg gcgtcgacgc
      181 agacaggccc ggacgcggtc acgatcaccg ggttgaggtc gagctccgcg acctggggcc
      241 agtcgatggc cagctgtccg atctgctgca gtgcccgcgc caagccgtcg acgtcgtact
      301 gctgcgcgcc gcggaagccg gtcaacagcg gccaggtccg gagtgcctgc aaggcaacga
      361 gtgcctcgtg gctggagaac ggcggcagca ggaaggtcgg gtcgttccac aacgccaagt
      421 tcgtcccacc ggtcgcgacc atcaccagcg gaccgaagcc ggcgtcacgg acgatgccga
      481 gggccagttc gggaccggcc agctgttgct gcaccaacac cggtccaggg cactcggcct
      541 ggatcgactc gacggcaccc gcgaccgctc cgccgtccct gagctggacg tgcacgaggg
      601 aacgctcgat cttgtgcacg acgtcaggac gaccggtctt ggcaaccacg ggatagccca
      661 tgccatctgc ggcgcgcact gcgtccgcag gacttgacac gacctcgaac cggacggtgg
      721 gcatcccgta gtcggccaac aagcccgcgg catctggcgt ctccatccag cccggggtgg
      781 ccggaggtac ccatgttccc gggacacgga cgggctcgtc gacggggacc tccgccgtac
      841 gccgccaggc ggagtacgcc gctgcgtggt gcaacgcgcg caccgccgaa tccatcgact
      901 cgaacggtgt cgcacctccg tggctggcgg tgacctcact gccgatgacc acgagagcgc
      961 agggcttggt gtgatgcgca gtgaccgccg catcgacggc agcgacaacg tcacccagat
     1021 cggaaaccgc agtcgccgcg accacgacca gcagcgcgtc cacctcggtg gacccgagca
     1081 acacgctcac cgcgccggcg aagcggtcgg acgaggctcc cgcgccgagg tccaccggat
     1141 tgctggtgcc tgcgacgccg ctgccgaggt ccgccagttc cgtctgcaat gcctcggaca
     1201 gggcggggac ggtcagcgag ctgttctgcg tcgcatcagc cgccagtacg ccgaggccac
     1261 cggcattgct gaccagaccg atacgggagc cgtcaggcaa tggctgctcg tccagcaagg
     1321 ccgctgtgtc cacgaggtcg gccacgccgt ccaacgcgat cacgccggcg gctctgaaca
     1381 cggcgtcaag ggcgcggcgc ggggtggcac tggcagccgt gtgtgaggcg ccaccgcgtc
     1441 ggccggccgc cgacgtaccg ccgaagacag cgagcagcgg cttcgcgtgg ccgaacgacg
     1501 atgccagccg gacgaatttc gacgggttgc cgaacgactc gagatacagc gcaccgaccc
     1561 ggatgtcggg atcctcctcc cacgctgcga gcaggtcgtt gccggacacg tcgagcttgt
     1621 tacccagcga cacgaacgcg gcaagaccca ggttccggcg gctggccgcg tccatcaacg
     1681 cgatgccgac gccaccggac tgcgagccga tcgccagacc gccggcaccc ggacgggcgg
     1741 tgccgaatgt cgcgttgagc cgcgtgtccc gcagtctgct cagcaccccg aagcagttgg
     1801 gtccgaccac acgcatgccg gcacgcctgg caaccgccgc tacttcacgc tcggcctccc
     1861 tgcccgactc gcctgtctcg gcgagcccgg acgtcacgat cacgcagacg cgggtaccgc
     1921 gaatggcgac tcgttcgacg atgtccagga cgtacgccgc gggcacggtc accacagcga
     1981 ggtcgagccc gatcggcagc tcgtcgacgt cggcgagcgc ctcgacaccg tcgatcgtca
     2041 ggtctgcgtg tccgacagcg agcagtcggc cctcgaagtt ctccgacctg atgttctcga
     2101 cgatttcgcg gccaatgccg cctcggcgcc ggctgacacc gatgaccgcg acggcagacg
     2161 gctcgaagag aggccggagc gacgcgcgtt cggcgtgccg ttcgcgccga tcggtggccg
     2221 cttgtgaccg ccgggtcggc cggacgtcga gcgtgacact gacgacgccg tgatcctggt
     2281 gctgttcgag gtcgaagccg gcgtcccgga agacccgcag catcggcagg ttgtcgctga
     2341 gcacctcggc gtggaacacg tcgatgccgt ggccgtacgt ccaggccgcg agatgctcga
     2401 gcatgagggt tccgatcccg aagccgtgct tggactcgtc gacgaacagc gccacctcgg
     2461 ccgagtcgct gccgtcaccc tccgcggtga cgaggccgac gatctgccca ttgagcacgg
     2521 ccaccagtga gatcgtgtcc gactcccccg agcagacgtg ctcgacgtag cgggccgcct
     2581 ggtcacggtt gagggcgaag aatcggcgtc ggatgctctc gtcggaggct tgggcgtgca
     2641 gcctcaggag tccctccttg tcggagggct ccaccgagcg cacctcggcc accgaaccct
     2701 cggcgagaaa cgcctccgaa acgtagggcg gcaaccctga ctcgttgtgc tcaccgcggt
     2761 gggtcgtctg cttgttcgtc tgtgccgtcg ccacgacgca cccctctgct gtcgatctcc
     2821 gctgcggaca tggatggacg agccggccat gagccggctc gtccatcatc gacccgttac
     2881 ttgaggatcg ggtacttctg ggtgatggcc agaccctccc accaccggcc gaggccgagg
     2941 tagcgccctg cgtagtacag gccgaccacc aggacggcgg cagcgccgag gatgtggtcg
     3001 tccgtgatcg ggttgttctc gggaggcagt acgacggtcc acatgagcag gtacatgaga
     3061 actccggcga acgtgccgag ccgcatgaag atacccagcg tcagcgagat accgatggcg
     3121 gcaagaccga acatgaaggc ccagtcggcc caggtgtcgc cggcgatcga gtggtagaag
     3181 tcctcgaacg gaccgtccgc gccgaacttc aggaagccca gcgtcggtga cccgccgtgg
     3241 atccacgccg cgtcgccgaa ccggtcgacg ttgcccgtct ccgggtcccg tcccgtggaa
     3301 tagcccagcg ccaacagctt gtcgaagaac gcccagagga atgtccaccc gaggaccagg
     3361 cgcagcacgc ccatcgcgat gtgcgctgcc ctccggctgg aggaaacctc aacggcgcct
     3421 cccgcaccct tctccagggt gctggtgtcc ttgatggtgt tcatggtgcc ctcctcgtcg
     3481 gatgaagggg gcggctgccc tctcgacacc agcctcgcgg ggggtcgctg tgatcacgag
     3541 cggccgaagt caccactgcg gggtactaaa gtcccagggt cgacacaacg gactattcgg
     3601 aagcgaggcg gctgctgccg gtgagctgcc gggcaagctc gacaccccac tggcgggcac
     3661 gctcctcctc gcccgcaacg agatggttct cccggtccac caggaagctc tccggcttga
     3721 cgagaagtcg gcacccatgc ttgccaagca tcttcgcgag accatgggag gcgcgtccgg
     3781 tgatgagcgc gggcatgtcc gttcgcgtgt cgaacgcggc cgccgccacc gacaacgaac
     3841 ccgtcgactc gaaccactca cgcaggccgt cgctcgaagc cccaggctcg agtgcaaggt
     3901 cactgtcggg ttcctgtgca gcttggacgg cggcgtctcg cgtacgccgc cggctgacgc
     3961 cgtgcacatg agtgggaccc ccgacgacga tgaggtcggc tccgcggacc atgcggacca
     4021 tatggacgtc gagctcgccg accgcggcca cggacacctc gcaagcctga gcgagcccgg
     4081 cagcgatcgc gttcgcgacg cggtgggtgt tgccgtacat ggactcgtag atgaccacgc
     4141 ccctcatgcg gccacgatga cgccgcaacc tgctgtgcgg cagaggcgat ggtcccgaag
     4201 cgctgcgcca gaggtcagtc gcccggccat tcgccggtcc agcgctcgaa cgctcgcgcg
     4261 ccgccccggt cgaccgccgc cttgatgacg gcgaagatcg caccctgcac ggccgccgcg
     4321 atgaggattt cgcggaagtc gtactccgac tccaacgcgc cgggtgcctc gtcgtctcca
     4381 cggacatggc gccagatctg cttgaagatg atgcccgcca gggcgccgcc gatcagcccc
     4441 gagaccaggc cgacgggccg gtaggcaatc ttggccgcct tgttcgaccg tctgtcctct
     4501 cgtgccacca gacctccctc ggtattccgg tcacgcgtcc gaccctcggt gccgccgcgc
     4561 gcaccgcgcc aacggccgcc ggcgagcggt gcggattccc gtcgccgctc ccctgccagc
     4621 ggcgacggga accgaagacc cacatgccag ccggggagca tgccccgttg acatggggcg
     4681 actccggcgc gcttggtgct gaacgccgaa gcctcgaccc accgatgtct cgagccatcc
     4741 gcggccgtgt cggttccgta ccccgatccg gctgagggca tgcacgggtt gcggatgatt
     4801 cgtgcctatc cgtacgccag ggtgcggcag cagtgagctc ggcatgggca gcgttgggct
     4861 catcgcatcg ggtctacagt gacgtaggtg gagtccgaga tccttgtcga gctgttcgag
     4921 gcggacgccg accccgaaca actcgacgac gaaacccgtc ggctgcggcg ggacctgctg
     4981 gagctcgacg tcgactccgt cgtacccgcc tccgcgggcg aggcgccgcc aggcacacgt
     5041 gcggtggacc ttgccgcgat cggtgcgctc gtcgtgacga tgcgcggtgg agtcgatctg
     5101 gtcaccaagg tcgtttcggt cgtgcagtcg tggctgcacg gtggcgccag gcctgatcgc
     5161 acgttgaaga tgacggtggg cgacaacacc atcgagctca ccgatcccac cgacgagcag
     5221 cagcagctgc tcatcgacaa gttcgtgcag caggttgccg ctgaggccta gcggctgccc
     5281 cacgccgggg tattcggccg ggcgtcttgc aaacccgtcg aaggatcgat gtcgatcgtc
     5341 gtcgctgagt ccgagcccgg cgtccggacg acgagcccgc caccttgggc ggtgacttcg
     5401 acgtactttt ggccgcctgg ggaccaggtc gctgaggctg cgagatcggg accttcctga
     5461 acgggttggt cgctgccgtc ggcgagtgtg tagacgtatc ccggcttgag gtggttgctg
     5521 gcgttgagcg acagacctcg cttgatgtac agcagcccgc ccgacgacga ccagtccagg
     5581 tcggagaagg aggcgccccc ttgcccgtcc tcgttcaccg cggtgatcgg ggtgtcaccc
     5641 gaaccgtcgg gatgcacacg gttcaacacc gccggtccgg tcggctcgtc tgtctcgagc
     5701 tcggagtaga tgatccactg gttccgctgc cacgtcggcg cacgggagag tgcgtcgctg
     5761 tcgaggacgc tctcgaggtt ccggccgtct gcgtcggcga cgtacagccc ggtcgatcga
     5821 ccgtcactgc ccatgcagac cacggcgagc cgcttcccgt cattgctgaa cgccggacgc
     5881 gagctgtact cgcattgctg gtccccgaac aactcgcggt cgtcactgcc gtcggagttc
     5941 atgatgtgca ccttgccgaa cgagtggtcg tcggtgggtg aatccgtcag gtagagaatc
     6001 tgccttcgat caggtgagac gacgggatac aggagctggt tgccggcgtc cacgctcgtg
     6061 atcacgtcga cgtcgccgtt ggtggtgtcg acgcgggaaa gacgtacgcc gtcatcggtc
     6121 tggaacgcga ccaccaggtc ctcctcgggg atcgcgcccg tcgtcgactc cggccacagc
     6181 gcaaccgccc ccgcaatgac caccacgagc agcaccgccg ccagcgatgc aagcacggcg
     6241 cggtgattgc gtcgtagcca ggctcgccac ccggacggtg gtggcttcgg tggtggcttc
     6301 ggtggtggcg gcggtggtgg tggcggctcc gtcccgaggg ctgccgacgc tgccgcgacg
     6361 acggtcttgc tgtcgtcggt cgcgaccaac gacgtgagtg cctcgcgcgc cgcgagggcg
     6421 agcccttggt gctctccgtt cagcatgcgg gccaactcgg gcacgaccgc gacccgcaca
     6481 ctcgacagcg ggttctccag tgcctctcgg accgagtcag gcagctcact cggcgtgtcg
     6541 accggcgtgc atcgacgagc gatgtacagc gtcccctgga agtctcccca cttgcccgga
     6601 gtctggtgct gcgtccgttc gcgcacgcgc tcgtagacgt agtcgtacag ctcgtcgagc
     6661 gagaccatgc cgtcctggtc gcggtcggcg tcgccggtcc gcagaccctc gacgagcgcc
     6721 ccggtgaaga ccgacggttg ggtgtcatcc tcgagcgctt ccagggccga gccgtccggc
     6781 agggccgcgg actcgtcgac gatggggaag actcctgtct cgtcgatggc cacctccatc
     6841 gcggtcgacg cggtgatcac cgcccggccg cgaccgccca gccgctcgtt gatgtcgacc
     6901 ccagtgccgc cgcgcgtccg gatgcggcgg tcgaatgccc cggcgtagca gcagtccagc
     6961 agcagcacga cccggcgaga ccgcgtgtgg tccatcgacc ggcagacgaa gtccgacgcg
     7021 accgacgtgg cctcgaggag gtcgaggcga gtgtccgccg tggcgaggta gagctccccg
     7081 ctgtcgtcct tgacgccgtg acaggagaag tgcagcagga gcaggtcgtc gggacgccga
     7141 tcgttgaaga acgtcgccac ctcgcgcaac acgatgtgcg ccggcttgtt gatcaccgtg
     7201 cggacgtcga acgctccgat gtccggatgg ccgagaacct cggcgagctc ctcggcgtcc
     7261 ttcgcgggag cacgcagccg ggtcagcccg cggtcggcgt actcgtagtt ggcgacgatc
     7321 agcgcattgc gcgccccgtc catcgtcctc acccccaacg ggcaggtagt gtcaccgtgc
     7381 gctcaccgag cgcagttgtc aacgcgatgg cgtccgcccg accctccgcg aattgcggga
     7441 aacgtctcct ccgtggcggc gtgtcgaccg ccactttccc gcaattcgcg ggaagccagg
     7501 cagggcagaa ggtcaggggt cggagggttc ggccgaaagg tctgcggtcc ggaggacgga
     7561 ctgcgcagag ctcgttgcgc cgatgtggtc gagaccgagg agtccggcgc cgatgaccgg
     7621 tggagtgtcg acgagccgcg tgacggcgta cggcgccacc gcggccagca gcgagtcgat
     7681 cgtctgcatg agcagcggat gaccggcggt gagtacgccg cctccgagga gcacgtcgat
     7741 cggctcgtcg gcgacctcga gccgacgcag cgccgccacg accagggcca cgatctcacc
     7801 ggcctgccga cgaacgatct cgaccgcgat gggatcgccg gactcggcca cttcgaagag
     7861 cagcggcgtc atgtcgaggc agcgctcggc gggaatgacc tcgagatgga tcgcctcgat
     7921 gagtgcctgc atcgagcgca ggccgaacat gtcgggcagc gcggtccgca accgggtgtc
     7981 ctcgcccctg ccgtcctcgg cgcgggccgc ccaccacatg gcttcctgcc acaggtagcc
     8041 accaccgccc cagtcgccgg agatccgccc cacggccgcg aaccgcgccg ttcgtccgtc
     8101 cggcagcatt ccggtgcagt tgatgccggc accacagatc acggcgatcc cgcgcggtac
     8161 ctccactcct gcacgcagca gcgcaaaggt gtcgttgacg acggcgctcg actcgcccca
     8221 ccgttctgcg tcgatcgcct ccttcaggag acgttgttcg atcggcaggt cggcgttggc
     8281 aaggcacgcc gagacgtgct ggacgaacgg cgtacccttc tcgaactcca gccccgcctc
     8341 ggcaaccgct ttcgagacca gcgggacgag gccggtgacc gcgtcctcgg ccccgatcag
     8401 atgcggcacg aacccttcac cccgggtggt gccgagcacg gtcccgtcaa cgctgatgat
     8461 cgccaggtct gtcttgctgt tccccgcatc gatcgccagc acgcccggtg tcgcgtcgga
     8521 gcgcacatcc gcctcggatt cagtcatgtg gtcacgccca cgacaggtag cggcggttgt
     8581 gggcgatcag ccggtcggtc agcgcggacg ccttgtcggt ctggcccacc agtgggtgcg
     8641 cgagcagagc ctggaagacg cgttcgcggc caccgaggag ggcagcgtcc aacgccagcc
     8701 gctcgtacgc cgtcacggcg gcaacgagcc cggcgaagag cggctcgagc ggctcgacgg
     8761 gaagcgggtg ggcgcccgcg cggtcgatcc gggccgggac ctccacgacg gcgtcctccg
     8821 gcaggaacgg tagtgtcccg gcgtttcgga cgttcacgac ctggaccggc gcaccggcgg
     8881 cgtcctcccc agtcggcagc ggagccagca acggagccag caggtcgacg gcagcctccg
     8941 agtagaacgc gccgccgcgc tcggcgagct gggccggctt ctcgtccagc ccggggtcgg
     9001 cgtacagctc caggagctcg cgctcgatcg cggccacctg cgacgcccgt gtgggtctgg
     9061 tgagcatctc gcggacgacc tcgtcgtgtg catagaagta gcggaggtag tacgacggca
     9121 cgacgccgag ctgcgtcagc acgtcggccg gcagctggat gtcggcagcg agctcggcag
     9181 cgtggtcggc aaggagcatc ggcagcatgt cctccccgtc gacacggacg gcacgctccc
     9241 acgtcagatg gttgaggccg acgtggtcaa ggttgacctc atcgggcgca atacccaaca
     9301 tccccgcgaa gcggcgctgg aagccgatcg cgacgttgca gagcccgatc gcccgatgtc
     9361 cggcctgaag gagggatctc gtgacaatgc cgaccgggtt cgtgaagtcg acgatccagg
     9421 cgttggggtt gctgcggcgt acctgctcgg cgatctggag gaccacgggc acagtccgga
     9481 tggccttggc gagcccaccg gccccagtgg tctcctgacc gatcgatccg cactcgagcg
     9541 gccaggtctc gtcctcgttg cgcgcctgct gtccaccgac ccgtagttgc agcaggaccg
     9601 catcggcatc ggctgtgccg gcctcgaggc tggacgtcca cgtcaggtgc gcaccatgtc
     9661 cctgcctggc gaggatgcgt cgtgcgaagt cgccgacgat ttcgagccgg tcgagcgcgg
     9721 gatcgaccag gaccagctcc tcgaccggga gcgtgtcggc gcggcgcgcc agcccgtcga
     9781 ccagctctgg cgtgtacgtc gagcccccgc cgacgacagc gagcttcacc ccttcacccc
     9841 cgtcagcgtc acgccttcga tgaaggcctt ctgagcgaag aagaacacga tgatgaccgg
     9901 cgccatcacg agcagtgtcg cggccatcgt caggttccag ttcgtgtggt gcgcgctctt
     9961 gaaggactcg agcccatagc tcaacgtcca ggcaccaggg ttctcgctgg cgtagatctg
    10021 cggcccgaag tagtcgttcc agcagtagaa gaactggaag agggcgaccg cggcgatcgc
    10081 gggcttcgac atcgggacca cgacgcgcag catcgcgcgg aagtcgccgc agccgtcgac
    10141 cttcgcagcg tcgatgtact ccgacgggat cgtgagcagg aactgccgga gaaggaagat
    10201 ggagaacgca tcgccgaacg ccatcgggat gatcagcggc cacaacgttc cggacagatg
    10261 gaactgcttg gcccagacca ggtacatggg cacgatgacc acttgtggtg gaagcatcat
    10321 cgtggcgatc acgatcccca tgcacagact gcgacctctg aaccggaacc gagccaaggc
    10381 gtaggcgaca gggaagctcg acaggatcgt gagaaccgtg ccactgacgg cgtagacgat
    10441 cgtgttgcgc caccaggttg cgaacccggg ggtctgccag gtgtcgacga ggttgtgcca
    10501 cgcccacggc tccggccaca ggttgcgggt cagcgtctgc tgatcggtca tcaacgacgt
    10561 cagcgcgatg aacacgaacg gcaaggtgaa caacagcgcg agcgcgacgc cgacggcatg
    10621 cagggcgatc cactccagtg tgggagtacg ccgctgcgtg cgcggcggcg gcaattcggt
    10681 cggcaacgcc gcggccgacc ggaggtcgga ggcgactgtc gtgttggcgg ccatcagatg
    10741 tcacccaaga gaccggagtt gcgccgcatc aggaatgcgg tgaacaccat cgacatggcg
    10801 aagagcacga gcgcgacgac gcacgctgat ccggtgtcga accgttggaa ccccaggttg
    10861 tagacgagct gcggaagggt cagcgtcgac ttctcgggat atcccggctc gaactgctgg
    10921 ccggacccgc cgatcacacc gctcgccacc tttcctgcga ccagtggctg cgtgtagtac
    10981 tgcatcgtgt agatgatccc cgtcacgaca gcgaacaaga cgatcggtgc gatgttgggg
    11041 agggtgatgt agcggaactg cttccacgcg ccggcgccgt cgagctctgc cgcttcgtac
    11101 tgctgcttgg gaacatcgag cagcgccgcc atgaagatca ccatgaggtc gccgatcccc
    11161 cacagcgcga gcagggtcaa agcgggcttc gaccaggtgg ggtcgttgaa ccagcccggc
    11221 tccgggagcc cgagcttccc gaggatgacg ttgaccggac cggtgccggg gttgagcagg
    11281 aacgcgaagg ccatcgttgc tgcgaccggt ggagcaaggt aggggacgta gaacagcgtc
    11341 cggagcaggc ctgcgcccgt cttgatcttg atcaccagcg tcccgatggc gagcccgaag
    11401 atcacccgga gcgtcaccat cacgatgacc agccagagcg tgttacgaag cgccggccag
    11461 aagaacgggt agtgctggaa gacgtatctc cagttgtcga ggccgttgta gacgggcggg
    11521 gtgaagccgt cgtacttggt gaacgagaag tagaccgtgg agacgagcgg atagatgaag
    11581 aacaagcccg tcccgagcag ccacggcgag agaaaggcga cgttgcgcac gaagtcgcga
    11641 cggcgcttcg tgcgcaacgt caccgggcgg gtcaccgcgg gggtcaccgt cgctgtcgca
    11701 ggggacgtcg atcgcctcat tgggcctgtt cgatgtcggt gtcgatctgg tccgcggtct
    11761 tgtccagacc ggcttgcaga tcggtcacct tgccggactc ataggagtag ccgaggtcct
    11821 gaatggtttg cgtgtacgtg ccaccgtccg gactcgccgg cgtggtgctg ctgtgcggat
    11881 tctgtgcgat ctcgatgaac gtcttcatcg ccggagactt gtcgaggtcg gaagacgcga
    11941 gggcgtccat cgtcgagggg acgttgtgga tcgcgttggc gaagctgacg acggcctgcg
    12001 tgtcggtcgt caggtacttc acgaactccc aggctgcgtt ctgcttgtcg ctgatgctcg
    12061 cgatcccgat gatcgtcccc gacaggtagc ccttgccata cgtgtcggcc tggctgtccg
    12121 gaacggggaa cggggcgacg ccgtactgga agtcgacgtt ggcgtcgtcg atgaacccgg
    12181 cacgccactc gccgtcgagc gccatcgcga cctggccggt ctggaacggg ttctttgcgc
    12241 cgaactcgtc accgaaggtc gtgcggtact tctcgagctt gtcgaaacca ccgagcgtgt
    12301 cgaccatgtg cttctgccac tcgaacatgt ccttgatggc ggggtcggtg ttgaccgtcg
    12361 acttgccgct gtcgtcgaag tacgtcgggt tccacatcgc ggcgtagtga tccgtggtcg
    12421 tctcgtagcc gtggtagttc ggcatgaagc cgagctgcga gtacgagtcg ccgtcggact
    12481 tcgtcagctt gacggcgtcc gcctcgaact ccgacagcgt cttcggcggc tgcttgatgc
    12541 cggctgcggc gaacatgtcc ttgttgtagt acaggccgta cgcgtcgccg agcaggggca
    12601 acgagcactg gtcgccttgg tactgcgtgt actcgagcat cggcttcggg aacgtcgagg
    12661 cggggtcgat gccgcccttt tccatgaacg ggttcaggtc ggtgaacacg tgtgacgcgc
    12721 agaacttccc gacgttggtg gtggtgaacg acgacaccac gtccggagcg ttgtccccgc
    12781 cggcgcgcag cgcctggttg atcttgtcgt cggtgatgtt gccgacgacc ttgacgtgga
    12841 tattggggaa cttcgcctcg aacctgtcga cgttctcctg gatcgccttg acctcgctgg
    12901 gggcgctcca gccgtgccag aaagtgatcg tggtctcctc gttcggattg tcgttcgcgc
    12961 cgccgctgct gctgccggtg caagcggtgc ccaccagtgc gagcagggcg gctgccgccg
    13021 tcccgacggc cgcgcgctga accttgtgca ttcgtgccat ggctccttct cctgttcaac
    13081 tggtcagggc gtgctgctga cggctggggt ggactggatg gtgtcgaaga cttcgtcgcg
    13141 agttcgtacc agcgcgacgt ggagggcacc gcacagaact ggcttctcgg gtatgccgga
    13201 cagctcgatg gtgggacgct gcaccgcgag tgccgccacc tcggtgcgga cttcggccag
    13261 taaccgggag ccgccggcct tcaagacatt ccccgtgagc acgaccagct cagggtcgag
    13321 gaccgaaacg atggcggcca gcccgagtgc gacccggtgg gcgagtgact cgatcagccg
    13381 gtcgccggcg ccgggtgtgc tcagtgcccg ggccacagct gcctccggag taccagcacg
    13441 gaaaccgagc tcgcgggcga gctcgagcac ctgcttccca ccggcgagct gctggaagcc
    13501 gccggcgttg ttgcggccga cgtcacgaac gagcggagtc tccggaagcg gaaggaaccc
    13561 cacctccccg gctcccccac tggccccacg gtggagcacg ccgtcgatca cgagggccgc
    13621 gcccagacct tcgtccgccc acagcagtac gaaattcggg tggccgcgtg cgcgaccact
    13681 gtgctgctca gcaacagctg ccaggttgac gtcgttgtcc acctcgaccg ggaggccgag
    13741 cgtgtcggcc agcctttcca ggatctgcgg gtcgtgccag cccggaaggt gtcgggcata
    13801 gcgcagccgc tgcgtcctgg gatcgaatgc gcccggcgta ccgatcacca cgcgatgcag
    13861 tcgcgatcgc tcgacgcccg cttgccgcaa cgcaccgtcg acagcggagc agacgcgctc
    13921 gaccgtgtcg gcgccggccc gacggggagt gcggagctcg tgctcgccga ccacctgccc
    13981 cgtgacgtcg gcgacggcag agaggatccg gcccggtgtc acgtcgagag cggcgacgta
    14041 cgccgtatcg ggattgatct cgtagagctg ggctttcggg ccaggacgcc cgacactgct
    14101 gccggtgtgc atgacgagcc cgacgcgctc gagccgtgcc agcagttgtg aagctgtcgg
    14161 tttcgacagg ccggtcagcg cgccgagctg gctgcgcgac aacggaccgt gctcgagtag
    14221 caggtccaac gctgcccggt cgttgatgcc ccgaagcagc cgcggcgtac cagcctgagc
    14281 cgtcatccga acctcgactt ccgggctgga cacatgattg ttaggaaagt ttcctaatga
    14341 atgccgcgag cgtatgacgc ccggcgcccc ctgtcaacgg tgcacgtcct ccccccgacg
    14401 gtcgagactg tagagacccc ggcgctcccg gctgtcgagc ctgtcgagac ccggactcac
    14461 aaggacgact ctcccttgat ctcgacaggt ggtacctgag cgtgtcgaag gactcgatca
    14521 gcggacttcg acggtccttg atcccgacaa gctcgatcag cgggtggcgg cggtcgagcc
    14581 tgttcagacc ctgatgccga tctcgacagg ttcctgagct tgtcgaagag ctcggtcagc
    14641 gggaagcccg gtgggcggcg agttgctcgg ctgcggcgat gatggcggcg ccggtcatgt
    14701 cgagggcggg ctcgtcggtc tgccacgaac ggacgtcgtc gacataccgg ctcccccgcc
    14761 cgttgaacgg cttgaacgga tcggccgtac cgggacagtg gaccatgcca tcctggtagc
    14821 caccgagtcc acccgcgaag atccgggcgt cgttgggtcc gttgacgacg gcaccgacat
    14881 cgagcgggcg gctcccgtcg gtgcttccct cgaggttggc gatctggtgc tgcatgcagt
    14941 ggggaaatgt ctggccgatg ccgaccatcg cagccacgcc ccacgggttc tcgccgagca
    15001 accagttgcg ccaggacgtc gcgaaccggt cgtaggagct gtgatgggga ccggagaggc
    15061 ggtggtagat cgccgcggtt gcgacgagcg caaacgcgtg cgagtttgca tcgaagttgt
    15121 cgtagatgac gcctgaggca aatgggtcgc gccgggagcg tgacacccct gtctcgagct
    15181 ggtgctggat gtctcgcatc agatcggccc gcgacaccgc cagccctgac atcccggtcc
    15241 ggctcaacgc gttgaccagc gcggcgtggg ccagcgcgct ggtgtcgtac aggttgagcg
    15301 tgtccccgta gcggctacgg atgtacgccc gtgcccagtg gccgctcgcc acgacgtacg
    15361 ggcgggcgtg ctcgccgagg cgccgtgacg ccagggcgat ctcggccgca cccagctcca
    15421 tgtcgtcctg ccagctgtct tccgggtaga acgcgtgcgg aagggctgtg accagttgct
    15481 tgggcggatg cctcgtcgcc gcacgcgcaa agagcagctt cgccttcgtc agctcgtggc
    15541 gtgcccggcg tggatgatct ctcgcgtccg ccacggcggc gagtgcgaac gcagccgaca
    15601 ctcggcccac gaggttcggg ctgatgcgct tgtgcggcgg ggcggcgcgg aacaccgggc
    15661 ggtgcgacac gtagcggtcg atgtgcctgg tgttgtggtc gtcggcctgc gggaggcgcc
    15721 acccgtcgtg gtcgccacgg aacgtccctg cccggttacc cgagccgacc cccacctgca
    15781 tgtagaggac gccgtccctc ggccgccaca tcttgtccag ccactggatt ccgtgcctgg
    15841 cctcggcgat cagcagctcc tgcagccgtc cgcggacgag ctgggcgctg gtgaagagca
    15901 gaacgtcgtt gtacgccgtg ctgtgcgcga acttcaggta gtcgccggcg tcgtaccacc
    15961 ctcccgatac atccaccgga ccgccgatcc gatgcagatc gcggtcggtg atgagatccg
    16021 agccgggcac gaagtggggc tttgcgtaga cgaaggcgcg tcggtcgtgg cggtgtgcag
    16081 gcttgcggtt cagggcacca ggtatgacgt gaaggccgtc gcgttggacc tggtcgaact
    16141 tcacgccgtc gtgcaggatc ctgccgtaca gccgcgtggc ggacacgatg ccgaacggtg
    16201 gtgacgacac cacgacaggg cctcgggtga cgaggtgata ccgcccaggc tcggtgaggc
    16261 cgtcgaacct gagccggtag acgacccggt agttgtcgtt ccagcgcccg accggatccg
    16321 cggggacgcg gccacggaag accaccatgc cgtgccggtc gaccactcgg aaccgctcgc
    16381 ccgagatccg gtgcgccacc atcagccgcg cccgcttggc ctcgtcaggc aggtaaccga
    16441 gttgatccac gcgaacctct gccgcgatat ggacgacgac tggtcgacgt tgtccgctcg
    16501 gcggggagga ggcggtcgac gcactgattc ccgcggccac cagagctgcg gaacccatga
    16561 gcgcgacgac gatccggcgt gagcgcattc cgcctgccct atcgatagga aactttcctg
    16621 tcgaatatgc atcctgccgc gcccacccgc aagaggcacc gcagcgcgac ttgtcgagac
    16681 cccttgatct cgacaggctc gatcagcggg ctccggcagg ccccatgatc tcgacaggct
    16741 cgatcagcag agtccggcgg tcgagcttgt cgagaccccc ttgatctcga caggctcgat
    16801 cagcagattc cggcggtcga gcttgtcgag acccccttga tctcg
//
LOCUS       DATBFZ010000236         4910 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_1139799, whole genome shotgun sequence.
ACCESSION   DATBFZ010000236 DATBFZ010000000
VERSION     DATBFZ010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 4910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4910
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1139799"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /locus_tag="VH419_07100"
     CDS             complement(<1..484)
                     /locus_tag="VH419_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002521708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY7043417.1"
                     /translation="MTRVTYVAAANRYESLPYRRCGRSGLQLPALSLGLWQNFGDDRP
                     LETQRAIIWRAFDLGITHFDLANNYGPPYGSAETNFGRIVAEDLKTYRNELVVSTKAG
                     WDMWPGPYGDLGSRKYLLSSLDDSLTRMRLDYVDIFYSHRFDPDTPLEETMGALDSAV
                     R"
     gene            complement(501..1571)
                     /locus_tag="VH419_07105"
     CDS             complement(501..1571)
                     /locus_tag="VH419_07105"
                     /EC_number="1.3.1.32"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018506 - maleylacetate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetate reductase"
                     /protein_id="HEY7043418.1"
                     /translation="MGAPRSFALDVLRQRVVFGRGSVQQVPKLVGDLGMSRVLVLASA
                     SATSAADGLAATLGDVVVGRLHEARQHVPEALAGEATRLASDSDADGAVTIGGGSATG
                     LGKAVAVATGVRLVAVPTTYAGSEATPVYGITGTHKKTGRDERALPTAVVYDAELTTS
                     MPAHVTATSGLNAMAHCVEAVYANGANPVTTLMAEEGVRLLARSLPAAVQEPHDVAAR
                     EDALFGAYLAGWSMATAGSALHHTLCHVIGGSYDLGHGDVHSVLLPHVAAYNAAAATE
                     PLGRLAGILDASDLASGLLELAKTVGAPMSLVELGMPPDGLGDVVDRTVEAIGGRNPR
                     PVDPTSLGEMLQRAYDGAPPAP"
     gene            1693..2073
                     /locus_tag="VH419_07110"
     CDS             1693..2073
                     /locus_tag="VH419_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043419.1"
                     /translation="MSTTEPTTRRARLTRNESELSDSRASMEVVVGSERLGSRQRMVT
                     DMDMARRHGGLTQVAACTVMAAHRRDHDHSVDRDGCHPHAVEVVHLGAQAVMVCHDCR
                     ADSGFIAHRDAEQLATLHRHDTAA"
     gene            2145..2474
                     /locus_tag="VH419_07115"
     CDS             2145..2474
                     /locus_tag="VH419_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY7043420.1"
                     /translation="MDVVSIPTLAEDQLTVATAAPSGRSAIRVYGRPTAGLRQTLLAL
                     DAGSKLGEHKSPGDASLHCLQGQVTVHAGEASIELRQGDLLALPPQRHDVEAHEPSVL
                     LLTVSLH"
     gene            complement(2495..2662)
                     /locus_tag="VH419_07120"
     CDS             complement(2495..2662)
                     /locus_tag="VH419_07120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043421.1"
                     /translation="MNKTWHRAHILRSGASMDERIKWHVAHAKACACREIPKSVREAI
                     AARDEQAARER"
     gene            complement(2720..3103)
                     /locus_tag="VH419_07125"
     CDS             complement(2720..3103)
                     /locus_tag="VH419_07125"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEY7043422.1"
                     /translation="MLYLDTSALVKLVFAEAESTVLARLVGDRDVAVSSLSRTELARV
                     ALRLDRRYITACHELIESCYELRLEPALLDRAGVAEPAALRSLDAIHLTSALTLGNSL
                     ESFVAYDARLLEAAAGASLPCLSPA"
     gene            complement(3107..3388)
                     /locus_tag="VH419_07130"
     CDS             complement(3107..3388)
                     /locus_tag="VH419_07130"
                     /inference="COORDINATES: protein motif:HMM:TIGR01552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HEY7043423.1"
                     /translation="MEHMERIGIRELRQHASRWIKRVEQGESFEVTDRGRPVAVLAPV
                     PSSESPVERMLRSGVLRAPTSLRRPRRRIRATQGPSASEVLAELRREDA"
     gene            complement(3414..>4910)
                     /locus_tag="VH419_07135"
     CDS             complement(3414..>4910)
                     /locus_tag="VH419_07135"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY7043424.1"
                     /translation="LLSTETRRLSARHQTLQGAIDWSYERLSPQEQALFTMSSVFAGG
                     WTLDAARAMSVAGGDGDVLELTSRLVDKSLIAKLDWRGRARFFMLGPLRQYGQAMLAR
                     SGQVSKVRDLHAAYFLEVAEEAETRLRGADQEDSLNLLEDEVDNLRSALGWATKEGQH
                     EVALRLAGALTMFWYLHGRYSEGREWLDRALLDETVQPDVWRAKGLVASGTLAALECD
                     YDVAEARTQAGLDLYAELGDRAGVASALQRLGSLAREQGRYEESTRLHDESLHVWREL
                     GDEAGIATSLNFLGFVAWLEADLDKATGHCSQALEVFQRLGDGEGLCWSLISLGAIAQ
                     YRGSLGQAENLLNGALTTARDLGFTEGVAWSLNQLGLVARRRGDFETSWNLARESLDQ
                     NRTLGDRWRAASVLEELAATAIGLDEPRRAARLLAAAGSVRRAIATPIPICERVDHDE
                     TVAAVGGRLPRTTLAADLRTGRRTALDRIDSLIDGPDREPSTGSHTQV"
BASE COUNT          762 a         1777 c         1613 g          758 t
ORIGIN      
        1 gccggaccgc gctgtcgagc gcacccatcg tctcctccag cggcgtgtcc gggtcgaacc
       61 ggtggctgta gaagatgtcg acgtagtcaa ggcgcatccg tgtaagcgag tcgtcgaggc
      121 tggacaacag gtacttgcgt gagccgaggt cgccgtacgg tcccggccac atgtcccatc
      181 ccgccttggt cgagacgacg agctcgttgc ggtacgtctt caggtcctcg gcaacgatgc
      241 gaccgaagtt ggtctcggcc gagccgtacg gagggccgta gttgttggcc aggtcgaagt
      301 gtgtgatgcc gaggtcgaac gcccgccaga tgatggcacg ttgtgtctcc agcgggcggt
      361 cgtcgccgaa gttctgccac agcccgagcg agagtgcagg gagttgcagc ccgctcctcc
      421 cacaccggcg gtacggaagg gactcgtaac ggttcgcggc tgctacgtag gtcacccgcg
      481 tcagtctgcc gcagcgtgac tcacggtgcc ggtggagctc cgtcgtacgc gcgctgcagc
      541 atctcgccca gcgatgtcgg atccaccggc cgcgggttgc gaccgccgat ggcctcgacc
      601 gtgcgatcga cgacgtcacc gagcccgtcc ggcggcatgc cgagctcgac caacgacatc
      661 ggtgctccga cggtcttcgc cagttccagc agaccgctcg cgagatcgga tgcgtccagg
      721 atgccggcga ggcgcccgag cggctccgtc gctgcagccg cgttgtacgc cgcgacgtgc
      781 ggcagcagca ccgagtggac gtcgccgtga ccgaggtcgt agctgccacc gatgacgtgg
      841 cagagcgtgt gatgcagggc gctgccggcg gtggccatcg accagcccgc caggtaggcg
      901 ccgaacaggg cgtcctcacg ggccgcgacg tcgtgcggct cctggacggc tgccggtagc
      961 gaccgggcca gcaggcggac cccctcctcg gccatcaggg tggtcaccgg gttggcgccg
     1021 ttcgcgtaca ccgcctcgac gcagtgggcc atggcgttca agcccgacgt cgccgtcacg
     1081 tgggcaggca tcgacgtcgt cagctcggcg tcgtacacga cggccgtcgg cagcgcgcgt
     1141 tcgtcgcggc cggtcttctt gtgcgtgccg gtgatgccgt acaccggagt ggcttccgat
     1201 ccggcgtacg tggtcgggac cgcaaccagg cgtacgccgg tcgccacggc cactgccttc
     1261 ccgagccccg tcgccgaacc tcccccgatc gtgacggctc cgtcggcgtc ggagtccgac
     1321 gccagccggg tcgcttcgcc ggcgagcgcc tctgggacgt gctgacgcgc ctcgtgcaac
     1381 ctgcccacca ccacgtcacc caacgtcgct gccagcccgt cggcagccga cgtcgcggac
     1441 gccgaggcga gcaccaggac ccggctcatc ccgagatcgc cgaccagctt cggtacctgc
     1501 tggaccgatc cgcgaccgaa gacgacccgc tgccgcaaga cgtcgagcgc aaagctgcga
     1561 ggcgctccca tcgtgacctc ctcgctgccc atcctccccc gaatcgcggg aggttccgcg
     1621 attttcggcc tcgagatccc ggagcctccc gcaatccgcg gggcaggtat cagccctgcc
     1681 ttcccttcgt ccttgagcac gacggaaccg acgacacgcc gagcccgtct gacccgcaac
     1741 gagtcagaac tgtcggactc gcgtgcttcg atggaagtgg ttgtcgggag cgaacggctc
     1801 ggcagccggc aacggatggt gactgacatg gacatggctc gacgtcacgg cgggctcact
     1861 caggtggccg cgtgcacggt gatggccgcc caccgacgcg accatgacca cagcgtggac
     1921 cgcgacggct gccacccgca cgcggtggag gtcgtgcacc tcggagcgca ggctgtgatg
     1981 gtctgccacg actgtcgcgc ggactcgggt ttcatcgccc atcgcgacgc cgagcagctc
     2041 gcgacgctcc accggcacga caccgcggcc tgagcgccga gggactccag ccctcgaccg
     2101 agatcaggct gtccacgtcc agcggcggct gacatcatgc acctatggat gtcgtgtcga
     2161 ttcccacctt ggccgaggat cagctcaccg tcgcgaccgc cgcacccagc ggccgcagcg
     2221 ccatccgtgt ctacggtcgt ccgacggcgg gcctgcggca aaccctgctc gcactcgacg
     2281 cgggcagcaa gctcggcgag cacaagagtc ccggagacgc gtccctgcac tgcctgcaag
     2341 gtcaggtcac cgtgcacgcc ggcgaggcga gcatcgagct gcggcagggt gacctgctgg
     2401 ccctgccgcc acagcgccac gacgtcgagg cccacgagcc atcggtcctg ctcctgaccg
     2461 tctctctgca ctgatccacg gcggcggact cgcttcaccg ctcccgggct gcctgctcgt
     2521 cccgtgctgc aatcgcctcg cggaccgact tcgggatctc gcggcaggcg cacgccttcg
     2581 cgtgggcgac gtgccacttg atccgctcgt ccatcgacgc accggaccgg aggatgtgtg
     2641 ctcggtgcca cgtcttgttc atgactcgat cgttgccccg atcgccggcc attgtcgagc
     2701 cgtcgtacgc cgatcagcct caggcgggac tcagacacgg cagcgacgcg ccggcggccg
     2761 cctccagcaa gcgtgcgtcg tacgccacga aggattccag cgagttgccc aacgtcaacg
     2821 cggacgtgag gtggatggcg tcgaggctac gcagcgctgc cggctcggcc actccggcgc
     2881 ggtcaaggag tgccggctcg agtctgagct catagcagga ctcgatgagc tcatggcatg
     2941 ccgtgatgta tcgccggtcc aagcgcaggg cgacacgggc cagttcggtc cggctgaggc
     3001 tgctcaccgc cacgtcccga tcgcccacaa gacgggcgag cacagtcgac tccgcctcgg
     3061 cgaagacgag cttcaccagg gcggaggtgt cgagatagag catctgtcag gcatcttccc
     3121 ggcgaagctc tgcgagcacc tctgaggcgg acggcccctg ggtcgcccga atgcgccggc
     3181 gcggcctgcg caatgacgtc ggtgcacgca ggactccaga acgaagcatc ctctccacag
     3241 gggactccga cgacggcacc ggcgcgagca ccgccaccgg ccggccccgg tcggtgacct
     3301 cgaacgactc gccttgctcg acccgcttga tccaccggct ggcatgctga cgcagttcgc
     3361 ggatcccgat gcgctccatg tgctccatgt gctacatggt agcacctggg gcgtcagact
     3421 tgagtgtgac tgccggtgga cggttcacgg tccggtccgt cgatcaagct gtcgatccgg
     3481 tccagcgccg tacgccggcc ggtccgaaga tcggcagcca aggtcgtgcg aggaagtcgg
     3541 cctcccactg ccgcgacggt ctcgtcgtgg tcaacccgtt cgcagatcgg tatgggtgtg
     3601 gcgatcgccc ggcgtaccga ccctgccgct gcgagcagcc gcgccgcccg ccgcggctcg
     3661 tcgagcccga tggcggtggc ggcgagctcc tccagcacgc tcgcggcacg ccatcggtca
     3721 cccagggtgc ggttctggtc gagcgactcc cgcgccagat tccacgatgt ctcgaagtcg
     3781 ccccgacgac gggcaaccag accgagctgg ttgagtgacc acgccacacc ctcggtgaac
     3841 ccgaggtctc gagccgtcgt caacgctccg ttgagcaggt tctccgcctg gccgagcgac
     3901 cctcggtact gcgcgatcgc acccaggctg atcagcgacc agcagagccc ctccccgtcg
     3961 ccgagccgct ggaacacctc gagcgcctgg gagcagtgtc ctgtcgcctt gtcgaggtca
     4021 gcctccagcc acgcgacgaa tccgaggaag ttcagcgacg tcgcgatgcc ggcctcgtcg
     4081 ccgagctcgc gccacacgtg caagctctcg tcgtgcagcc gcgtcgactc ctcgtaccgg
     4141 ccctgctcgc gcgcgaggct cccgaggcgc tgcagcgccg acgcaacgcc ggcccggtcg
     4201 cccagctcgg catacaggtc gagtccggcc tgggtacgtg cttccgcgac gtcgtagtcg
     4261 cactcgaggg cggcaagcgt gccggacgcc accagcccct tggcccgcca gacatccggc
     4321 tgcacggtct cgtcgagcag cgcccggtcg agccactcgc gtccctcgct atatcggccg
     4381 tgcaggtacc agaacatcgt cagcgccccg gcgagccgca gcgccacttc gtgctggccc
     4441 tccttcgtgg cccaccccag cgctgagcgc aggttgtcga cctcgtcctc gagcaggttg
     4501 aggctgtcct cctggtcggc gcccctcaac cgggtctcgg cctcctccgc cacctcgagg
     4561 aagtacgccg catgcagatc ccggaccttc gacacctggc cggacctggc gagcatggcc
     4621 tggccgtact gacgcaacgg gccgagcatg aagaagcgcg cacggccgcg ccagtccagc
     4681 ttggcgatca gtgacttgtc gacgagtcgc gaggtgagct cgaggacgtc cccgtcgccg
     4741 ccggcgaccg acatcgcgcg ggcagcgtca agcgtccagc ctccggcgaa caccgacgac
     4801 atcgtgaaca gcgcctgctc ctgcggcgac aagcgctcgt agctccagtc gatcgcacct
     4861 tgcagcgtct ggtgccgcgc ggacaaccgg cgagtctcgg tgctcagcag
//
LOCUS       DATBFZ010000237         3884 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_1139840, whole genome shotgun sequence.
ACCESSION   DATBFZ010000237 DATBFZ010000000
VERSION     DATBFZ010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 3884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3884
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1139840"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            94..462
                     /locus_tag="VH419_07140"
     CDS             94..462
                     /locus_tag="VH419_07140"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY7043425.1"
                     /translation="MPNAVVDLAQKLSAFSEHWSPKVVARLNDYEIKVVKVQGEFTWH
                     SHADTDELFLVISGELTIQMRDGSVSLRPGQLFVVPRGVEHCPIAEGEVHAMLVEPTG
                     VVNTGNAGGPLTADHDDSLA"
     gene            895..2355
                     /locus_tag="VH419_07145"
     CDS             895..2355
                     /locus_tag="VH419_07145"
                     /inference="COORDINATES: protein motif:HMM:NF014214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M36 family metallopeptidase"
                     /protein_id="HEY7043426.1"
                     /translation="MTKPRKLVVALTAALATISLGLSFTSVSGAAPEATRASGLKLIM
                     TNTSLLGTHYWYQQTFKGLPVINGYYARHVTKFGGVQIADGRDAVPADLDVSANIPSS
                     RAIQSANAVVSARAVGGGTAAPDKSITSTPTATQGAAQLAVVGGPNARLVWNVTSTSA
                     EGVSRTLVDANTGSVLQSKVISDNVRGRGSVFDPNPVVSEQNERLTDKNDRNQHALFL
                     AQKTVILRNLDGSGRLRGSYVDIVKAKGGLARSSNNQFLYQRENNKFEQVMAYYHVNQ
                     TQEYIHQLGFTQVNNESQDFEINTFAGDNSFYDPTSDTITMGEGGVDDAEDAEVIWHE
                     YGHAMQDDVVPGFGETLEAGSIGEGFGDYWAVTMSEPVSNGFELPCVMDWDATSYTST
                     EPHCLRRTDGNKTTDDIVGEVHDDGEIWSRALWDIHQALGRTKADKLILEATFFYDPD
                     ASFAGAARDTVQSARLLYGKSAAKKVTDAFKARKIL"
     gene            complement(2436..2511)
                     /locus_tag="VH419_07150"
     tRNA            complement(2436..2511)
                     /locus_tag="VH419_07150"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2476..2478),aa:Arg,seq:acg)
     gene            2933..>3884
                     /locus_tag="VH419_07155"
     CDS             2933..>3884
                     /locus_tag="VH419_07155"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020578783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase
                     domain-containing protein"
                     /protein_id="HEY7043427.1"
                     /translation="MTAETTETASSTDAPSTASGIDPHDSVAPSNTSTTHAGLQAWVD
                     QIAALTTPDRVHWCDGSEEEWETLTTELVANGTLVQLDPAKKPNSFWAASDPTDVARV
                     EDRTFICSLDESDAGPTNNWMAPAEMKATMTDLYRGCMRGRTMFVIPFVMGHLEAEHP
                     MFGVEITDSAYVVASMRIMSRMGTQVLRKIEELGADADYVPCIHSVGMPLEAGQQDAA
                     WPCNGTKYISHFPEERAIWSYGSGYGGNSLLGKKCYALRIASAKARDEGWLAEHMLIL
                     KLISPENKSYYVAAAFPSACGKTNLAMLQPTIEGWRAETVG"
BASE COUNT          730 a         1297 c         1246 g          611 t
ORIGIN      
        1 ccaggagcac cttcacatga ggaggtgaga ttgacctgga cgtgctgcct tcggtcgaag
       61 tgctgaggtc gtgcgccgtg cgaacatgat cacgtgccaa acgctgtcgt agacctcgcc
      121 cagaagctgt ccgcgttctc cgagcactgg tcgccgaagg tggtcgcacg tctgaacgac
      181 tacgagatca aggtcgtcaa ggtccaaggc gagttcacgt ggcacagtca cgcggacacc
      241 gacgaactgt tcttggtgat cagcggcgag ctgacgattc agatgcgtga cggcagcgtc
      301 agtctgcggc cgggccaact cttcgtcgtg ccccgcgggg tagaacactg tcccatcgcc
      361 gagggggagg tgcacgccat gctcgttgaa cccactgggg tggtcaacac gggcaacgca
      421 ggcggtccac tcacggcaga ccacgacgac tcactcgcgt agccgcatcc gcagatccgt
      481 agcagccggt cgtcctcacc gggcgggttc cgatgactac gctgatctgc accttggtcg
      541 acccgctcgc ccgatcggac ctccaggtct ctgcggtgac gagggcttct cgcgaagcgt
      601 cgtttgcctc cgacggcgtc cacgcttccg gcgaagtcct tgcccagaac gaccggaaag
      661 cggtgctcca tcatgcgttt gatcgctagc gtgaacgcgc tcgtcctgtg cggtcctggc
      721 cggcccgatc cgcgcacccc atccgccccc ctggccattg gtcgacaggt cgccgccgag
      781 aaatgggctg ggacttcgct tcccagcggg tgcccgggcg aagtaatgtc cgagccggcc
      841 gtggttgttg cgtgatgtac cgccgacgac ttcgttggca atgggagaga acgtatgacg
      901 aagccccgca agctggtcgt ggccctcacc gccgcactgg ccaccatttc actcggactg
      961 tcgttcacga gcgtatccgg ggccgcgccg gaggcaacca gagcgagcgg cctgaagctg
     1021 atcatgacga acacctcgct gctcggcacg cactactggt accagcagac attcaaaggt
     1081 ctgccggtga tcaacgggta ctacgccagg cacgtgacca agttcggcgg cgtccagatc
     1141 gccgacggac gggacgccgt gccggccgat ctggacgtgt ctgccaatat cccctcgtca
     1201 agggcgatcc agtcggccaa cgctgtggtc agtgcccgcg cagtcggggg cgggaccgcc
     1261 gccccggaca agagcatcac ctccacgccg accgccaccc agggcgccgc ccaactcgcg
     1321 gtcgtcggcg ggccgaacgc ccggctcgtg tggaacgtga ccagcacgtc cgccgagggc
     1381 gtcagccgga cgctggttga cgcgaacact ggatcagtgc tccagtcgaa ggtgatcagc
     1441 gacaacgtcc gcggccgcgg ttcggtcttc gaccccaacc cggtggtcag cgagcagaac
     1501 gagcggttga ccgacaagaa cgaccggaac cagcacgccc tgttcctcgc gcagaagacc
     1561 gtgattctcc gcaacctgga cggctctgga aggctccggg gcagctatgt cgacatcgtg
     1621 aaggccaagg ggggcctggc gcggagcagc aacaaccagt tcctttacca gcgcgagaac
     1681 aacaagttcg agcaggtgat ggcgtactac cacgtcaacc agacgcagga gtacatccac
     1741 cagctcggct tcacccaggt gaacaacgag tcgcaggact tcgagatcaa caccttcgcc
     1801 ggtgacaact cgttctacga tccgacgtca gacaccatca cgatgggcga gggtggggtc
     1861 gacgacgccg aggacgccga ggtgatctgg cacgagtacg gtcacgccat gcaggacgac
     1921 gtcgtaccgg gcttcggtga gacgctcgag gccggctcga tcggtgaggg cttcggcgac
     1981 tactgggccg tgaccatgtc cgagccggta agcaacggct tcgagctgcc ttgcgtgatg
     2041 gactgggacg cgacgtcgta cacgtccacc gagccgcact gcctccgtcg taccgacggc
     2101 aacaagacca ccgacgacat cgtcggcgag gtccacgacg acggcgagat ttggtcgcga
     2161 gctttgtggg acatccacca ggcgctcggc cgaaccaagg ccgacaagct gattctcgag
     2221 gcgaccttct tctacgaccc cgacgcctcg ttcgccggcg ctgctcgtga caccgtccag
     2281 tccgcccgac tgctctacgg caagtccgcg gcgaagaagg tcaccgacgc gttcaaggca
     2341 cgcaagatcc tgtagcacgc acaccaggcg cacccccgat caccgcggct gggcgcaaga
     2401 ccgaggtcaa cgacgggttt ggcgactgcg tgatgtggcg cgctcggagg gattcgaacc
     2461 cccaaccttt tgatccgtag tcaaatgctc tatccgttga gctacgagcg ccggcatggg
     2521 cgtgcctgcg ccaggacggg gaggaccggc gtcccggcgc gagcagtgag tttagcctgc
     2581 cgctccgctt cggatgaaat cgaccgggct cgaccaagga aggtcctccg ataccaaccg
     2641 ggatctgcct cggtaccggg ccacccgcat cggtaccgag gcagacctca gcggtcgaag
     2701 cgctgctcag cggccgtttt cgcgcagggt cgaacaggct cgcacaggtc gcacatgttt
     2761 gtgagcgtct tcacaaaggt ccggatggcg gacactcgtg caaccggcgt acggtgtcgg
     2821 aaaaccgccc ccagaaacca gactgcgggc gcccatatcc acatcttccg ccgttggaga
     2881 ccggcggccg gtggttcgtc cgccgcgatg cgcgagaggg aacattccag gcatgaccgc
     2941 cgagacaacg gagaccgcca gctccaccga cgccccgagc acggccagtg ggatcgatcc
     3001 ccacgactcc gtcgccccca gcaacacctc cacgacccac gccggcctgc aggcgtgggt
     3061 cgatcagatt gcggcgctga ccacgcccga tcgggtccat tggtgcgacg gctccgagga
     3121 ggagtgggag accctcacca cggagctggt ggccaacggc acgctcgtcc agctcgaccc
     3181 ggcgaagaag ccgaactcgt tctgggccgc gtccgacccg accgatgtcg cgcgtgtcga
     3241 ggaccgcacc ttcatctgct cgctcgacga gagcgacgct gggccgacca acaactggat
     3301 ggcgccggcc gagatgaagg ccacgatgac ggacctctac cgcggatgca tgcgcggtcg
     3361 gacgatgttc gtcatcccgt tcgtgatggg ccacctcgag gccgagcacc cgatgttcgg
     3421 cgtcgagatc accgactcgg cgtacgtcgt cgcgtcgatg cgcatcatgt cgcggatggg
     3481 cacgcaggtg ctccgcaaga tcgaggagct cggtgccgac gccgactacg tcccgtgcat
     3541 ccactccgtc ggtatgccgc tcgaggccgg ccagcaggac gccgcctggc cgtgcaacgg
     3601 cacgaagtac atcagccact tccccgagga gcgggcgatc tggagctacg gctccggcta
     3661 cggcggcaac tcgctgctcg gcaagaagtg ctacgccctg cggatcgcgt cggcgaaggc
     3721 ccgtgacgag ggctggctcg ccgagcacat gctgatcctc aagctcatct caccggagaa
     3781 caagtcgtac tacgtggcgg cggcgttccc gtcggcgtgc gggaagacca acctcgcgat
     3841 gctgcagccg accatcgagg gctggcgcgc cgagaccgtc ggtg
//
LOCUS       DATBFZ010000238        19001 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_483412, whole genome shotgun sequence.
ACCESSION   DATBFZ010000238 DATBFZ010000000
VERSION     DATBFZ010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 19001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19001
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_483412"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="VH419_07160"
     CDS             complement(<1..229)
                     /locus_tag="VH419_07160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043428.1"
                     /translation="MSNETTQGTVWAFDPDAQSGSVVLDDGTSLPFDARAFAVSGLRL
                     LRPGQRVRVEVSDGAVTGLTILTLRPSCQNPI"
     gene            complement(269..982)
                     /gene="cofC"
                     /locus_tag="VH419_07165"
     CDS             complement(269..982)
                     /gene="cofC"
                     /locus_tag="VH419_07165"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012922423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HEY7043429.1"
                     /translation="MSSQVARSNVMSGLATSPSPSPVHRVGTAQHAWSVIVPVKQTTL
                     GKSRLTGLADDERRALAVAFALDTVAAAVAADGVRRVVVVTNDPGADAFLELGAEVLA
                     DVPDAGLNPAIVHGAHQVAAQDVTTGLVALTADLPALTPEVLSAALAASPAPRWFVPD
                     ALGSGTTLLAAERERLAPAFGSRSRQAHRDSGAVELDMAGLHRLRQDVDTAADLWGAV
                     RLGVGAHTSAVLARRRRAS"
     gene            1080..1868
                     /locus_tag="VH419_07170"
     CDS             1080..1868
                     /locus_tag="VH419_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY7043430.1"
                     /translation="MARSRPQQKRGWAFALCAFVLRRPLMILTKRDWRGAENLPSTGG
                     CILVTNHVSEVDPVPFAHFVYDNGRLPRFLGKAEVFAVPVLGGILKNAGQIPVYRKSA
                     DASKAFSAAVAAVEKGECVIVYAEGTITRDPDLWPMVGKSGAARIALSTGRPVIPCAQ
                     WGPQELLAPYARKPDLFPRKLMRVRAGSPVDLSDLQGKKLTPAVLREATNRIMADVTH
                     LLEDIRGAKAPEARFDPRLAGVAEFGNPNKPKKPKAAPPETDAG"
     gene            1865..2866
                     /locus_tag="VH419_07175"
     CDS             1865..2866
                     /locus_tag="VH419_07175"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017934068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEY7043431.1"
                     /translation="MSKVAVFGAGSWGTAFSLVLADAGNNVVLWARREELSDAINSTR
                     ENPDYLPGLMLPDVVSATHDPEEALSDAEIVVLAVPSQTLRANLEHWAKHIPDDAVVL
                     SLMKGVELGTSKRMSEVIQEVTGIGHDRVAVLTGPNLSHEIAVRQPAASVVACDDEAV
                     AEFLQERCHTPALRIYTNTDVVGCELGGATKNVIALAVGMAVGLGFGDNSTATVITRG
                     LAETARLGVALGADPYTFSGLAGLGDLVATCSSSLSRNRTFGEKLGRGMSVDDAVAST
                     RQVAEGVKSCEPIKDLAVRAGVEMPIVEAVAALVRGETTPQEMLQALVSRATKPERD"
     gene            complement(2893..4017)
                     /locus_tag="VH419_07180"
     CDS             complement(2893..4017)
                     /locus_tag="VH419_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007078229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY7043432.1"
                     /translation="MTHTDDSRPDLPSGHDSHRSWRRETLAVKLGRPAVEPDQPLNEP
                     LTMASTYVAGGDREYGRYANPTWEAFENAVGALEGGRALSFASGMAAVSTLLDMVAAG
                     ETVVAPRHCYLGTLVQLAGLEARGLIKLVQVDIADTDTVLDAMNDDVALLWIESPTNP
                     ALEVADITTLVDGAHRAGVRVVVDNTFATPLLQHPLELGADVVVHSGTKYLSGHADAV
                     IGLVVTRTDEVFRALDERRRTIGALPGTLEAWLALRGLRTLHLRVERAQANAQELVRR
                     LTDHPALERVRYPGFGAIVAIEVRGGAAAADLVTHSTSLWVHATSLGGVESTFERRRR
                     WANEPHTIPEGLIRLSVGVEDVDDLWDDLRAALDLVARVD"
     gene            4087..5202
                     /locus_tag="VH419_07185"
     CDS             4087..5202
                     /locus_tag="VH419_07185"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007078228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HEY7043433.1"
                     /translation="MSTPRKPRVAVVFGGRSSEHAVSCSTAASVLAVIDREAYDVLPI
                     GISTEGKWVLESGDAERLALTSGHTPEVDASSAAVVVSQDPTRREIAVAEPGQTPHQL
                     GEVDVVFPLLHGPWGEDGTIQGLFEVAGIRYVGSGVLASATGMDKHYMKVVLAAAGLP
                     VMPHVVIKPREWKDDPAAAREAVDSLGYPVFVKPARGGSSLGITKVHGPDELDAAVEH
                     ARDFDPKVLVEPSAEDSREIECGVLERLEGGADASTVAEITGYGQHEFYDFAAKYLGE
                     EGVTLEVPARLREDLTSKVRELAVAAFEAFDCEGLARVDFFVGRDDSIVINEINTMPG
                     FTPASMYPRMWAESGISYPELVDRLIALALQRSTGLR"
     gene            complement(5360..5932)
                     /locus_tag="VH419_07190"
     CDS             complement(5360..5932)
                     /locus_tag="VH419_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020574802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3515 domain-containing protein"
                     /protein_id="HEY7043434.1"
                     /translation="MRPRFASSRAGGVVTHGAGRAGRIPWPLVLWPVVLWSVVLAGCG
                     SSNEAVEVTAPSPTGVVASQCRHLVDVLPDTVADDEQARDVTPSDAPAAAWGDPPVVL
                     RCGVPKPSALVPTSACFEVDGVGWLATQDGEEVSGDEPVSGTLTWTTIGRSAYVEVSV
                     PEADGRQPSDPLPALAPAIKQTIPLVHPCQ"
     gene            complement(5967..6200)
                     /locus_tag="VH419_07195"
     CDS             complement(5967..6200)
                     /locus_tag="VH419_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006602873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HEY7043435.1"
                     /translation="MVQAYILIQTEVGKAAEVAQNIADVKGVTLAEDVTGPYDVIVRA
                     EARNVDELGKLVVAKVQAIDGITRTLTCPVVHI"
     gene            6366..7334
                     /locus_tag="VH419_07200"
     CDS             6366..7334
                     /locus_tag="VH419_07200"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEY7043436.1"
                     /translation="MGQPPAGTLGDLGEFALVQALTSRFAAADDERVLVGPGDDAAVV
                     TVEGAHVVASLDMLVEGRHFRRDWSSATDIGRKAAAENLSDINAMGGRSTALLVGLGA
                     PGDLEARWAMEFADGLAEESAILAATVIGGDLTQADVVTISITALGFCEHGVVRRFGA
                     RPGDVVAMAGRQGWASAGYAVLARGFRSPRSVVDAHRRPQPPYDAGPQGAQLGATAMI
                     DVSDGLLQDIGHVATASAVAIDVDSRRFDVPEPLQAVGAALGVDPMRFVLTGGDDYGL
                     VATFPAGTELPQPWRQIGTVHDKGEPGVVTVDGEPYDDPTGHQHFR"
     gene            complement(7507..7692)
                     /gene="rpmB"
                     /locus_tag="VH419_07205"
     CDS             complement(7507..7692)
                     /gene="rpmB"
                     /locus_tag="VH419_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEY7043437.1"
                     /translation="MAAVCDICAKRPGFGHNVSHSHRRTKRRFDPNIQRVRAIVDGTP
                     KRLNVCTSCLKAGKVTR"
     gene            7956..9650
                     /locus_tag="VH419_07210"
     CDS             7956..9650
                     /locus_tag="VH419_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008358322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEY7043438.1"
                     /translation="MTDPVAAAALQLLQRWCDVTLDGLGAAREEIDALNVYPVPDGDT
                     GTNLYLTFEAARQALVDAEPSTLVAALTVFARGALLGARGNSGVILSQLMRAGADQLL
                     RGDPSKPGQLLADTLTLAADAAYDAVATPVEGTMLTVARVAADAAQAAVPEAPEDLSI
                     QMGYVVRAAATAARDALEHTPEQLAVLAAAGVVDAGGRGLCVILDCAEEVLTGRRPAG
                     GFRPRSPLPVPAPVGGDLAADGPAYEVMYLLDADDGAIPMLRQRLAPLGDSLVVVGGD
                     GLWNVHVHVDDVGAAIEAGIDAGKPHRVQVTHFAEQLSQAEAVRRRTGTADGAAPARG
                     VVAVGAGPGLVSLFSDAGATVVAGGAARRCSTGELLDAIRSTGAPEVIVLPNDGDSIA
                     VAEAAAQTARDEGVRVAVIPTKVQVQGLAAVAVHDPSRSFDDDVVHMTSAAGHARHGA
                     VTIAAKDAMTMAGPCRAGDSLGVVEGDFAIVGGAPDDVAVEVVSRLLSGGGEMVTLVT
                     GALEPAPGRTPGGAEPVDPAALVRRVASHLASTRPDVEVLVYDGGQRRYPLLIGVE"
     gene            9650..11863
                     /gene="recG"
                     /locus_tag="VH419_07215"
     CDS             9650..11863
                     /gene="recG"
                     /locus_tag="VH419_07215"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEY7043439.1"
                     /translation="MVGSDSPIRGIVGSRAANALKDVFEIETVGALLEHYPRRLEDRG
                     KLTDFRHLRLDEHVTVLAKTKQVDTKTYQPKGGGRLAARTEVVITDGERDLLCTFFRQ
                     AWRANQLVPGTVALFSGKVSVFNRRRQLTHPTCEVIGHGTELDSFEDAVQDASTRRWL
                     PIYPASAKLTSKMIMDSVELVLTAVDPLPDPLPEDVRRRHGFASYDEAIRTLHRPDSR
                     EAWGAARRRLAYDEAFVLQTVLAQRRMFTRSLQAMPRRRRPGGLLDRFDAALPFQLTR
                     GQREIGEQVGNDLEQPHPMHRLLQGEVGSGKTLVALRAMLQVVDAGGQAALLAPTEVL
                     AQQHHRSIVGMLGSMAEGGMLGGDDDATRVALVTGSLSAKRRKGALLDAVSGSAGIVI
                     GTHALLEQAVQFSDLGLVVVDEQHRFGVEQRAALTDKSGITPPHVLVMTATPIPRTVA
                     MTVFGDLDVSTLRELPLGRAEISSTVVPSSERPDWLARAWQRVHEEVDKGHQVYVVCP
                     RIGGEPESAMQEPVEDDPDERTGTRRPPLAVADVAAMLVEGPLADLRVFMLHGRLPAE
                     EKDDLMRRFAAGDIDVLVATTVIEVGVDVPNASLMLVLDADRFGVSQLHQLRGRVGRG
                     SVPGLCLLVTDALEGSRSRARLEGVAATTDGFELSRLDLEHRREGDVLGATQSGRRSS
                     LRLLSVLRDEDVIADARSAADALVQADPALAGHPELRRRVEALMQTEQADFLEKS"
     gene            12000..12623
                     /gene="rsmD"
                     /locus_tag="VH419_07220"
     CDS             12000..12623
                     /gene="rsmD"
                     /locus_tag="VH419_07220"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020574811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEY7043440.1"
                     /translation="MTRIIGGSAGGRRLRTPHGSTTRPTADRVREALFSSLESELGTV
                     AGIRFLDLFAGSGAVGLEARSRGASYVVLVEADRQTAALIRGNVRDLGFDDVEVHAMR
                     AERFVESLAHPGDDNAQDQPFDVVYVDPPYAMGTPELGEVLDNLVRSARVSDETVVVV
                     ERSRRGSGWNWPDAMRGVRERRYGETMLYYGRPAGATVVEPGSDPVP"
     gene            12638..13123
                     /gene="coaD"
                     /locus_tag="VH419_07225"
     CDS             12638..13123
                     /gene="coaD"
                     /locus_tag="VH419_07225"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017934053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEY7043441.1"
                     /translation="MRRAVCPGSFDPPTNGHLDIIGRAARLFDEVTVGVLVNPSKSSV
                     FSVDERLGMLRQLVADVDNVKVEAFEGLLVDFCRDHDVDVIVKGLRAVSDFDYELQMA
                     QMNSRLTGVETVFVPTSPEWSFLASSLVKEVARLGGDVSGLVPSYVLERMRERLDPAD
                     A"
     gene            13159..13710
                     /locus_tag="VH419_07230"
     CDS             13159..13710
                     /locus_tag="VH419_07230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043442.1"
                     /translation="MRHRDEGEEATVDVKQLIEQLRSTIEHARSMPMSSSAVINRAEV
                     LELIASLESVLPAAFEQSDRVFAERDSVLAEAEARAARIVEEAGLERDRLVSDTEVYR
                     IAVRESDSLRSGVEDECAALRKETDEYVDTRLANFEITLTKTLEAVSRGRDRLQGRNE
                     LDEESLDSLAALDDAGIPPLPQH"
     gene            13891..14457
                     /locus_tag="VH419_07235"
     CDS             13891..14457
                     /locus_tag="VH419_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HEY7043443.1"
                     /translation="MRTDPRSPFVINTHELGRRPGSMHTLRLTLPAPTDLGIELLGVP
                     PGSPIELDLRLESVMEGVLASGWARVLLTGECARCLEPIEDDLEVELQELYVYPESDA
                     EEDEASRLDGELLDLEPVLRDSVVLALPFRPVCQPDCEGLCPECGTRLLDDPGHAHEE
                     GVDPRWAALSALTTNDAGRADPTGKDEE"
     gene            14460..14645
                     /gene="rpmF"
                     /locus_tag="VH419_07240"
     CDS             14460..14645
                     /gene="rpmF"
                     /locus_tag="VH419_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008358292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEY7043444.1"
                     /translation="MAVPKRKMSRSNTRHRRSQWKAAAPSLVTCANPACGSKHLPHRA
                     CPECGQYGARADRRQVL"
     gene            14654..15472
                     /gene="rnc"
                     /locus_tag="VH419_07245"
     CDS             14654..15472
                     /gene="rnc"
                     /locus_tag="VH419_07245"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014352117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEY7043445.1"
                     /translation="MRPAVDDAALGPSRDELCAELGVDDLDPELLDRALTHRSFAYEN
                     GGLPTNERLEFLGDSVLGVVITESLYLTHPDLSEGRLAKLRAAVVNARALADVARTLG
                     LGDHIMLGRGEISTGGRDKSSILSDGVEAIIGAIYLDKGFDVAAGVIHRLFDPLMAAA
                     AGLGAGLDWKTSLQELSADLGLGVPEYLIDDSGPDHEKTFEARVRVGDGTYGHGTGRS
                     KKEAEQQAAETAWRAIRAAHPETGGRSPAPFEPGEAGPIASSDPHAPVAPGESA"
     gene            15505..16356
                     /gene="mutM"
                     /locus_tag="VH419_07250"
     CDS             15505..16356
                     /gene="mutM"
                     /locus_tag="VH419_07250"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012922373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HEY7043446.1"
                     /translation="MPELPEVEVVRRGLQAHVVGTTFEHVRVLHPRSVRRHVAGIADF
                     EHTLTGRGVVGVRRRGKYLWLALDNGDALLGHLGMSGQLLVLLRTAETGPHLRVVFRL
                     GDGRDLRFVDQRTFGVLLVSRDGAQLPAEIAHIARDPVDPLFDDSVFASAVRKRRTGI
                     KRALLDQSLVSGVGNIYADEALWAARLHYARPTETMNRTEIARLLAEVRAVLLRALGE
                     GGTSFDALYVNVEGESGYFDRSLAVYGREGRPCPRCGTPIRRDTFMNRSSFTCPHCQR
                     RPRRGRW"
     gene            16858..17061
                     /locus_tag="VH419_07255"
     CDS             16858..17061
                     /locus_tag="VH419_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012922372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043447.1"
                     /translation="MAKALLGYVGGDMRLLDDNQRLRRRIADLEAVVLRLQAENDALA
                     ARVHDGELLTVPDSMHERAAAHA"
     gene            17267..17737
                     /locus_tag="VH419_07260"
     CDS             17267..17737
                     /locus_tag="VH419_07260"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY7043448.1"
                     /translation="MSLHLVPMTTAEYDEWRGGAVSGYAQEFVDSGILQPTEATERAE
                     KDFAELLPDGLATENHLFWTAYVDQSDRVGTIWVALLPERQPPHSFVYALDVHPDHRR
                     RGYGQAIMEAAMQECRERGIATMGLNVFGHNDVARRLYDRLGFVVTSTSMKTTL"
     gene            17950..>19001
                     /locus_tag="VH419_07265"
     CDS             17950..>19001
                     /locus_tag="VH419_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020574818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY7043449.1"
                     /translation="MYLKSLTLRGFKSFASSTTLKLEPGITCIVGPNGSGKSNVVDAL
                     AWVMGEQGAKSLRGGKMEDVIFAGTSGRPPLGRAEVLLTIDNADGALPIEYAEVTISR
                     TMFRNGGSEYAINGQTCRLLDIQELLSDSGIGREMHVVVGQGQLDQILHASPEERRGF
                     VEEAAGVLKHRKRKEKALRKLDATEGNLTRLSDLLTEIRRQLKPLGRQAEIARRASVV
                     QMEVRDARARLLADDVVRVQESLQQELADEEALRGRRTEVEAALTTRRTGEAELEEAL
                     RADAPLLTKAQDTWFRLSGVRERLRGTASLAAERVRNLAEEPAEERAGRDPDELEAEA
                     VQVSAREAEITAEIVG"
BASE COUNT         2926 a         6421 c         6749 g         2905 t
ORIGIN      
        1 cgatggggtt ctgacaactc ggtcgcaggg tcaggatcgt gagcccggtg acggcgccgt
       61 cactgacctc cacgcgaacg cgctgtccgg gtcgcagcag gcgaaggcca ctcaccgcga
      121 acgccctggc gtcaaagggc aggctggtcc cgtcgtcgag gacgacgctg cccgactgtg
      181 cgtcggggtc gaaggcccac accgtgccct gcgtcgtctc gttgctcacg ccgcaacggt
      241 agtcgtccgg ccgtcgccgg atcgaggatc agctggcgcg tcgccgtctc gccagcaccg
      301 cactcgtgtg agcaccgact ccgagtcgca ccgcgcccca gaggtctgcg gccgtgtcga
      361 cgtcctgacg aagccggtgc aaccctgcca tgtcgagctc gacggcgccg ctgtcacgat
      421 gggcctgtct cgaccgagag ccgaaggcag gcgccaaccg ttcgcgctcg gccgcgagca
      481 gcgtcgttcc ggagcccagc gcatccggca cgaaccagcg aggtgccggg cttgccgcca
      541 acgcggcaga caacacctcc ggcgtcaacg ccgggaggtc tgcggtcagc gcgacgagtc
      601 cggtcgtcac gtcctgtgcc gcgacctggt gggcgccgtg cacgatggcc gggttcaggc
      661 cggcatcagg gacgtccgcc agcacctccg cgccgagctc gaggaacgca tcggcgccgg
      721 ggtcgttcgt caccacgacg acgcggcgta cgccgtcggc tgctaccgcg gcggccaccg
      781 tgtcgagagc gaaggcgacg gccagggctc gccgttcgtc gtcggcgagc ccggtcagcc
      841 gggacttacc gagcgtggtt tgcttgacgg ggacgatgac cgaccaggcg tgttgcgcgg
      901 tccctacacg gtgtaccggg gaaggcgacg gactggttgc aaggccggac atcacgttcg
      961 atcttgccac ctggctcgac atcgctgcgg atcgtgcacg acactgtctc ctgcccacga
     1021 ccctgacgag ccggcggatg cgcggcagcg gtcatcgaag gcaggcgtga aggagcaacg
     1081 tggcaaggtc aagacctcag cagaagcgcg gctgggcgtt cgcgctgtgc gcgttcgtgt
     1141 tgcgacggcc gttgatgatc ctgaccaagc gcgactggcg gggcgccgag aacctcccct
     1201 ccaccggcgg ctgcatcctc gtcaccaacc atgtctccga ggtcgacccg gtcccgttcg
     1261 cacacttcgt ctacgacaac ggccggctgc cgcgcttcct cggcaaggcc gaggtcttcg
     1321 cggttcccgt tctcgggggg atcctcaaga acgccggcca gattcccgtg taccgcaagt
     1381 cggcggacgc ctcgaaggcg ttcagcgcgg cggtcgccgc ggtcgagaag ggcgagtgcg
     1441 tgatcgtgta cgccgagggc acgatcaccc gtgacccgga cctgtggccg atggtcggca
     1501 agagcggtgc cgcccgcatc gcactgtcga caggccgtcc ggtgatccca tgtgcgcagt
     1561 gggggccgca agagcttctc gcgccgtacg cccgcaagcc tgatctcttt ccgcgcaagc
     1621 tgatgcgggt ccgggccgga tcgcccgtcg acctctccga cctgcagggc aagaagctga
     1681 cgcctgccgt actccgcgag gccaccaatc gcatcatggc cgacgtgacg cacctgctcg
     1741 aggacatccg tggagcgaag gccccggagg cgcggttcga tcctcgactc gccggcgtcg
     1801 ccgagttcgg caaccccaac aagcccaaga agccgaaggc ggcgccgccc gagacggatg
     1861 ccggatgagc aaggtagccg tcttcggtgc cggctcatgg gggaccgcgt tctcgctggt
     1921 gctggccgac gccggcaaca acgtcgtctt gtgggctcgc cgggaggaac tgagcgacgc
     1981 gatcaactcc acccgtgaga acccggacta tctcccaggc ttgatgctgc ccgacgtcgt
     2041 gtccgcgacc cacgaccccg aggaggcgtt gagtgacgcc gagatcgtcg tgctcgcggt
     2101 gccgtcgcag acactgcggg cgaacctcga gcactgggcc aagcacatcc cggacgacgc
     2161 ggtcgtgctg agtctgatga agggcgtcga gctggggact tcgaagcgga tgagtgaggt
     2221 gatccaggag gtcaccggca tcggccacga tcgggtcgcc gtgctgaccg gcccgaacct
     2281 gtcgcacgag atcgccgtgc gccaaccggc cgcgtccgtc gtcgcctgtg atgacgaagc
     2341 ggtcgcggag ttcctccagg agcgctgcca caccccggcg ctgcgcatct acaccaacac
     2401 cgacgttgtt ggttgcgagc tcggtggtgc caccaagaac gtcatcgcgt tggcggtcgg
     2461 gatggccgtt gggctcggct tcggcgacaa ctccacggcg acggtcatca cccgcgggtt
     2521 ggccgagacg gcacggctcg gggtggcgtt gggtgcagac ccgtacacgt tctcgggtct
     2581 ggccggtctc ggtgacctgg tggccacctg ctcgtcgtcg ctgtcacgca accggacgtt
     2641 cggggagaag ctcggtcgtg ggatgagcgt cgacgacgca gtcgcctcca cccgtcaggt
     2701 cgccgagggc gtcaagtcgt gcgagccgat caaggacctc gcggtccggg ccggtgtcga
     2761 gatgccgatc gtcgaggcgg tcgcggcgct ggtccgcggc gagaccaccc cgcaggagat
     2821 gcttcaggcg ctggtgtcgc gcgcgaccaa accggagcgc gactgacgca atcgcagtgc
     2881 tcgacagagc ggtcagtcga ctcgcgcgac caggtccagg gcagcgcgca ggtcgtccca
     2941 gaggtcgtcg acgtcctcga cgccgacgct caacctgatg agcccctcgg ggatggtgtg
     3001 cggttcgttc gcccagcgtc gacggcgttc gaacgtcgac tcgactccgc cgaggctcgt
     3061 ggcgtgcacc cagagcgacg tgctgtgcgt gacgagatct gctgccgccg ctccgcctcg
     3121 aacctcgatg gcgacgatcg cgccgaaccc ggggtagcgc actcgctcga gtgccgggtg
     3181 gtcggtcaac cggcgtacga gctcctgcgc gttggcttgg gcgcgctcga cgcgcagatg
     3241 caacgtgcgc aggccacgca gtgccagcca tgcttcgagc gtccccggca gggcgccgat
     3301 cgtgcgccgt ctctcgtcga gggcgcggaa cacctcgtcg gttctggtga cgaccagtcc
     3361 gatgacggcg tcggcatgac ctgacaggta cttcgtcccg gagtgcacca cgacgtcggc
     3421 acccagctcg agcgggtgtt gcagcagcgg cgtggcgaag gtgttgtcga ccacgacgcg
     3481 tacgccggcc cgatgtgcac cgtcaaccag ggtggtgatg tcggcgacct ccagcgccgg
     3541 gttggtcggg ctctcgatcc acagcagtgc cacgtcgtcg ttcatcgcat cgagaacggt
     3601 gtcggtgtcg gcgatgtcga cctgcacgag cttgatgagg ccgcgcgcct cgagcccggc
     3661 cagctgcacg agcgtcccga ggtagcagtg ccgtggcgcg acgaccgtct cgccggccgc
     3721 caccatgtcg agcagcgtcg acaccgccgc catacccgag gcgaacgaca gcgcgcggcc
     3781 gccctcgagc gcgcccaccg cgttctcgaa cgcttcccag gtcgggttgg cgtaacggcc
     3841 gtactcgcgg tcgccaccgg cgacgtacgt cgacgccatg gtcagcggct cgttcagcgg
     3901 ctggtcgggc tcgacggctg gtcgtccgag cttgacggcc agcgtctcgc ggcgccagct
     3961 ccggtgcgag tcgtggccgg aagggagatc gggacggctg tcatcggtgt gggtcactcg
     4021 cacattgtcg tcggtgctgg cctgtcgcac cgcgggcacc ctcaggacct cgttagagtc
     4081 gagccggtga gcaccccgcg caagcctcgt gtcgccgtcg tcttcggtgg ccggtcgagc
     4141 gaacacgccg tgtcctgctc gacggccgcc agcgtgctgg cggtcatcga ccgggaggcg
     4201 tacgacgtgc tacccatcgg catctccacc gagggcaagt gggtgctcga gtcgggcgat
     4261 gccgaacggc tggcgctgac ctcgggccac acaccggagg tggacgcctc ctcggcggca
     4321 gtcgtggtca gtcaggaccc cacccgacgc gagatcgcgg tggccgagcc tggtcagacc
     4381 ccccaccagc tcggtgaggt cgacgtggtc ttcccgttgc tgcacggccc ctggggggag
     4441 gacggcacca tccaggggtt gttcgaggtg gcggggatcc ggtacgtcgg gtccggcgta
     4501 ctcgcgtctg caaccggcat ggacaagcac tacatgaagg tggtgctcgc cgccgccggt
     4561 ctgcccgtga tgccgcacgt ggtcatcaag ccgcgcgagt ggaaggacga tccggcggcc
     4621 gcgcgcgaag cagtcgactc gctcggctac ccggtgttcg tgaagccggc acgtggtggg
     4681 tcgagtctgg ggatcaccaa ggtccacggt ccggacgagc tcgacgcagc ggtcgaacac
     4741 gctcgtgact tcgacccgaa agtgctcgtg gaaccgtcgg ccgaggactc ccgcgagatc
     4801 gagtgcggtg tgctcgagcg gctcgaaggt ggcgccgatg cgagcacggt cgccgagatc
     4861 acgggatacg gccagcacga gttctacgat ttcgcggcga agtacctggg tgaggaaggc
     4921 gtcacgctcg aggtcccggc gcgcttgcga gaggacctca cgtccaaggt ccgcgaactg
     4981 gcggtggcgg cgttcgaggc gttcgactgc gagggcctgg cccgggtgga cttcttcgtc
     5041 ggtcgcgacg acagcatcgt catcaacgag atcaacacga tgccgggttt cacgcccgcc
     5101 tcgatgtacc cgcggatgtg ggccgagagc ggcatctcgt acccggagct cgtcgaccgg
     5161 ctcatcgcgc tggcgctgca gcggtcaacc ggtctgcgct gacgggctga gccgagcacg
     5221 gagtcgtcgg gtccggctcg acaacctccc gcgcgtctcg gcggccaccc agagtctgta
     5281 tcgcacttcc cctgaatcca tcgtgggttt gcgatgtaaa cacgccgggc gggctgtaaa
     5341 catcgcaaac cgcctaggtt cactgacagg ggtggacgag tgggatcgtc tgcttgatgg
     5401 cgggggcgag ggcaggcagc ggatcggagg gctggcgtcc atcggcttcg gggacggaca
     5461 cctcgacgta cgccgagcgg ccaatcgtcg tccaggtcaa cgtgcccgag acgggctcgt
     5521 ccccgctgac ctcctcaccg tcctgggtcg cgagccagcc gacaccgtcc acctcgaagc
     5581 aggccgacgt cgggacgagc gcggacggct tcgggacgcc gcagcgcagc acgacgggcg
     5641 ggtcgcccca ggccgcagcg ggcgcgtccg acggagtcac gtcgcgggcc tgctcgtcgt
     5701 cggcgacggt gtcgggaagt acgtcgacca gatgtcggca ctgggacgcc accacaccgg
     5761 tgggagaggg cgccgtcacc tcgaccgctt cgttgctcga cccgcagcct gccagcacca
     5821 cggaccacag caccacgggc cacagcacga gtggccacgg gatccggccg gcccgcccgg
     5881 cgccgtgcgt gacgacgccc ccggctcgcg aggaagcgaa ccgggggcgc acaggagctg
     5941 atggaccgat gccgtggccg gacggatcag atgtggacga ccgggcaggt caacgtgcgg
     6001 gtgatgccgt cgatcgcctg caccttcgcg acgacgagct tgccgagctc gtcgacgttg
     6061 cgtgcctcgg cgcggacgat gacgtcgtac ggtccggtga cgtcctcggc gagcgtgacg
     6121 cccttgacgt cagcgatgtt ctgggcaacc tcagcggcct tgccgacctc ggtctggatg
     6181 aggatgtacg cctgtaccac catgtgcggg cctcctggct gagtcggggc tgagtcgaag
     6241 cagggtcggg gctgagacgg ctgtctggtc tcgtccgtgg cggtgctcac gctaccgctc
     6301 gtgacggcgt cgtcgcttcg ctaggttgtc acatcgacca gcaccgcgga agcgaggtac
     6361 gccggatggg acaaccgcca gcaggcacac tcggagacct cggcgagttc gcgctggtgc
     6421 aggcgctgac gagccggttc gcggcggccg acgacgagcg cgtgctcgtg ggacctggtg
     6481 acgatgcggc agtcgtgacc gtcgagggtg cgcacgtggt cgcctccctc gacatgctgg
     6541 tggagggcag gcacttccga cgggactggt cgagcgccac tgacatcggt cgcaaggcgg
     6601 ccgcggagaa cctgtccgac atcaacgcga tgggtggtcg ttcgaccgca ctgctcgtcg
     6661 ggctcggagc acccggcgat ctcgaggcga ggtgggccat ggagttcgcc gacggcctgg
     6721 ccgaggagag cgcgatcctc gcggccacgg tcatcggggg cgacctgacc caggcggacg
     6781 tcgtcacgat ctcgatcacc gctctcggct tctgcgagca cggtgtcgta cgtcgcttcg
     6841 gcgcgcggcc gggggacgtc gtcgcgatgg caggtcggca gggttgggcg agtgccggat
     6901 acgcggtcct ggcccgtggc ttccgatcgc cccgatctgt ggtcgacgct caccgcaggc
     6961 cgcagccgcc gtacgacgcc ggccctcagg gtgcccagct cggcgccacc gcgatgatcg
     7021 acgtcagcga cgggctgctg caagacatcg gtcacgtcgc gaccgcgagc gcagtggcga
     7081 tcgacgtgga cagtcggcgg ttcgacgtgc cggagccgtt gcaggcggtc ggggcggccc
     7141 tcggcgtcga cccgatgcgc ttcgtgctga ccggcggcga cgactacggt ctggtggcga
     7201 cgttcccggc cggcacggag ctgccgcagc cgtggcggca gatcgggacc gtgcacgaca
     7261 aaggagagcc cggtgtcgtc accgtcgatg gcgagccgta cgacgacccc accggccacc
     7321 agcacttccg ctagcccttt cccgccgagg tggcaacttt cgttggaaac acgccggttc
     7381 acgaacgtcg cttgccacct cggcgaaagc tgaagccaac cgaaagcccc cgggaccgga
     7441 cgggtccagg gggctcaggt ggcagctcga gatggttccc gcggccgagc cgccggggac
     7501 cgttcgtcag cgggtgactt tgccggcctt gaggcaggac gtgcacacgt tgaggcgctt
     7561 cggcgtaccg tcgacgatcg cgcggacccg ctggatgttc gggtcgaagc gccgcttcgt
     7621 acgacggtgc gagtgcgaca cgttgtggcc aaagccaggt cgcttggcgc agatgtcaca
     7681 gacggcagcc acggtgtact cctggagaag cgggtcggtt ggtcattcga gatgtcggtg
     7741 cccggaagcg gatccgtcgt gagcaccaca acgccggtgc gggcaacctc gaaaggatac
     7801 cggatcgccg ccgcggcacg ccaatcgaag gaacggctca ccggggcatc gaccgggcgc
     7861 agcacatcgg gtcacggaca gtcatgacct agggtttgag cagcagcgca acccaccggg
     7921 accgcccgcc gtcgtcctgc cctcggagac cgcccgtgac cgaccccgtc gccgcagcgg
     7981 cactgcagct cctccagcgc tggtgcgacg tcaccttgga cggactgggc gcggcacgcg
     8041 aggagatcga cgccctcaac gtctatccgg tacccgacgg cgacaccggt accaacctgt
     8101 acctgacctt cgaggccgct cggcaggcgc tggtcgacgc cgaaccatcg acgctcgtgg
     8161 cggcactgac cgtgttcgcc cgcggagcgc tgctcggcgc tcgcggcaac tcgggcgtga
     8221 tcttgtcgca gctcatgcgt gccggcgccg accagctgct ccgcggcgac ccgagcaagc
     8281 cgggccaact gctcgcggac acgctcacgt tggccgccga cgcggcgtac gacgccgtgg
     8341 cgactccggt cgagggcacc atgctcaccg ttgcccgggt ggcggccgac gccgcccagg
     8401 ccgccgtccc ggaggcgccg gaggacctgt cgatacagat ggggtacgtc gtccgggctg
     8461 cggcgacagc ggcccgggac gccctcgaac acacgcctga gcagctggcg gtgctggccg
     8521 ccgccggggt ggtcgatgcc ggcggccggg gtctctgcgt gatcctcgac tgtgcggagg
     8581 aggtgctgac agggcgccgt ccggccggtg gcttccgacc ccgcagcccg ctcccggtcc
     8641 cggccccggt cggaggagat ctcgcggcgg acggtcccgc ctacgaggtc atgtacctcc
     8701 tcgacgccga cgacggtgcg atcccgatgc tccggcagcg gctcgccccg ctcggagact
     8761 cgttggtcgt ggtcggcggt gacggcctct ggaacgtcca cgtgcacgtc gacgacgtgg
     8821 gcgccgcgat cgaggcgggc atcgacgccg ggaagccgca ccgtgtccag gtgacccatt
     8881 tcgccgaaca gctcagccag gccgaggcgg tgcggcgtcg tacggggacg gcggatgggg
     8941 cggcaccagc acgcggtgtc gtggcggtgg gcgcaggacc cgggctggtg tcgctgttca
     9001 gcgacgccgg cgccacggtt gtcgcgggcg gtgcggctcg ccgctgctcg accggagaac
     9061 tgctcgatgc gatccgctcg accggagccc cggaggtgat cgtgctgccg aacgacggcg
     9121 acagcatcgc cgtcgcggag gctgcggcgc agacagcccg cgacgaaggc gtccgagtgg
     9181 ctgtgatccc gacgaaggtg caggtacagg ggttggctgc ggtcgccgtc cacgacccga
     9241 gccggtcgtt cgacgacgac gtcgtgcaca tgacgtcggc ggccggacac gcgcggcatg
     9301 gcgccgtcac gatcgcagcg aaggacgcta tgacgatggc cggcccatgc cgggcgggcg
     9361 acagcctggg cgtcgtcgaa ggcgacttcg cgatcgtcgg aggtgcaccc gacgacgtag
     9421 ccgtcgaagt cgtcagccgg ttgttgtccg gagggggtga gatggtcacg ctcgtgaccg
     9481 gggccctcga gccagcaccc ggccggacgc ccggcggtgc ggagcctgtc gacccggctg
     9541 cgctcgtacg ccgggtggcg tctcacctcg cgagcacccg gcccgacgtc gaggtgctgg
     9601 tgtacgacgg aggccagcgg cgctacccgc tgctcatcgg agtcgagtga tggtcgggtc
     9661 ggactcgccg atccgtggca tcgtcggcag ccgggcggcg aacgcgctca aggacgtctt
     9721 cgagatcgag accgtcgggg cactgctcga gcactacccg cgccggctcg aggatcgcgg
     9781 caagctcacg gacttccggc acctccgtct cgacgagcac gtcaccgtgc tggccaagac
     9841 caagcaggtg gacacgaaga cctaccagcc gaagggcggc ggtcgactcg cggcccgcac
     9901 ggaggtcgtc atcaccgacg gcgagcgcga cctgttgtgt acgttcttcc ggcaggcatg
     9961 gcgggcgaac cagctcgtcc ccgggacggt cgccttgttc agcggcaagg tctcggtgtt
    10021 caaccgccgc cgccagctca cccatccgac gtgtgaagtc atcggccacg ggaccgagct
    10081 cgactcgttc gaggacgcgg tgcaggacgc ctcgaccagg cggtggctgc ccatctatcc
    10141 ggccagcgcc aagctcacgt cgaagatgat catggactct gtcgagctgg tgctgaccgc
    10201 ggtcgacccg ttgccggatc cgctcccgga ggacgtccgt cgccggcacg gctttgcgtc
    10261 gtacgacgag gcgatccgca ccctccaccg gccggacagt cgagaagcgt ggggcgcggc
    10321 gcgccggagg ctggcgtacg acgaggcgtt cgtcctccag accgtgctcg cgcagcgccg
    10381 catgttcacc cggtcgttgc aggccatgcc gcggcgccgg cgccccggcg gactgctgga
    10441 ccgcttcgat gccgcgctgc cgttccagct cacccgggga caacgcgaga tcggagagca
    10501 ggtcggcaac gacctcgagc agccgcaccc catgcatcgg ctcctccagg gtgaggtcgg
    10561 atcgggcaag acgctggtgg cgctgcgggc catgctccag gtcgtggacg ccggtggcca
    10621 ggcagcgttg ctcgccccga ccgaggtgct cgcacagcag caccaccgct cgatcgtggg
    10681 gatgctcgga tcgatggccg agggcggcat gctcggcgga gacgacgatg cgacccgagt
    10741 ggcgctggtg accggatccc tctcggcgaa gcggcgcaaa ggagcgctgc tcgacgccgt
    10801 gtcgggttcg gccgggatcg tgatcggcac ccacgcgctg ctcgagcagg cggtgcagtt
    10861 ctccgacctc ggcctggtcg tggtcgacga gcagcaccgg ttcggagtcg aacagcgtgc
    10921 tgcactgacc gacaagtccg ggatcacgcc accgcacgtg ctggtgatga cggccactcc
    10981 gatccctcgg accgtggcca tgacggtctt cggcgacctc gacgtctcca cgttgcgtga
    11041 gcttccgttg gggcgggcgg agatctcgtc gaccgtcgta ccgtcgagtg aacggcccga
    11101 ctggctcgcg cgcgcgtggc agcgcgtcca cgaggaggtg gacaagggcc atcaggtgta
    11161 cgtcgtctgc ccacgcatcg gcggcgagcc ggagtcggcg atgcaggagc cggtcgaaga
    11221 cgacccggac gaacgcaccg gcactcgtcg gccgccactc gcggttgccg acgtggccgc
    11281 catgctcgtc gaagggccgc tcgccgatct gcgcgtcttc atgttgcacg gccggttgcc
    11341 cgcggaggag aaggacgacc tcatgcgacg cttcgccgcc ggcgacatcg acgtactcgt
    11401 cgcgaccacg gtcatcgagg tgggcgtcga cgtgcccaat gcttcgctga tgctcgtgct
    11461 ggacgccgac cgattcggcg tctcgcagct ccatcagctg cgtggccggg tcggtcgagg
    11521 ttccgttcca ggcctctgcc tgctggtcac cgatgcactc gaggggtcgc ggtcgcgcgc
    11581 ccgactggag ggcgtcgccg cgacgacgga cggcttcgag ctgtcgcggc tcgacttgga
    11641 gcaccgccgt gagggcgacg tgctcggcgc cacccagtcc ggccgccgct ccagcctccg
    11701 gctgctttcg gtgctccgtg acgaggacgt gatcgccgac gcgcggagcg ccgccgacgc
    11761 gttggtccag gccgacccgg ccctcgcagg tcaccccgag ctgcgccgcc gcgtcgaggc
    11821 cctgatgcag accgagcaag ccgacttcct cgagaagtcc tgacccaccg accgacgctg
    11881 tgcctcccgc gaattgcggg aaacgtgctg ccaacacgcc gatggcaacg tgtactttcc
    11941 cgcaattcgc gggaacccgg caccgggcgg gacctgggag acaggcagga tgacgagtcg
    12001 tgacacgcat catcgggggg tcggcgggag gacgccggct gcgtacgccg cacggaagta
    12061 cgacgagacc gaccgccgac cgggtccggg aagcgctgtt ctcgtcgctg gagtccgagc
    12121 tcgggaccgt ggcaggcatc cgcttcctcg acctgttcgc cggatccggg gcagtggggt
    12181 tggaggcgcg ctcgcgcgga gcgtcgtacg tcgtgctggt cgaggcggac aggcagaccg
    12241 ccgcgctgat cagggggaac gtcagggatc tcggcttcga cgacgtcgag gtgcacgcca
    12301 tgcgggcgga acgctttgtc gagtcgctcg cccatccggg cgacgacaat gcgcaggacc
    12361 agcccttcga tgtcgtgtac gtcgaccctc cctacgcgat gggcacgccc gagctcggtg
    12421 aggtcctgga caacctcgtg cggtcggcgc gggtctcgga cgagaccgtc gtcgtggtcg
    12481 agcggtcccg ccgtgggtcc ggttggaact ggccggacgc gatgaggggg gtgcgtgaga
    12541 ggcggtacgg cgagacaatg ctctactacg gtcgtcccgc cggagcgacc gtcgtcgagc
    12601 caggctcaga cccggtccca tgagcgagga gtccgacatg cgtcgtgccg tctgtccggg
    12661 ttcgttcgac ccgccgacca acggacacct cgacatcatc ggtcgtgcgg cgcggctctt
    12721 cgacgaggtc acggtcggcg tcctggtcaa cccgagcaag tcgagcgtgt tcagtgtcga
    12781 cgagcggctc ggcatgctga ggcagctggt cgcagacgtc gacaacgtca aggttgaggc
    12841 gttcgagggc ttgctcgtcg acttctgccg cgaccacgac gtcgacgtca tcgtcaaggg
    12901 cctccgcgcg gtcagcgact tcgactacga gctgcagatg gcgcagatga actcccgcct
    12961 gaccggcgtc gagaccgtgt tcgtcccgac cagcccggaa tggtcgttcc tggcatcgag
    13021 cctggtcaag gaggtcgccc gacttggcgg cgacgtgtcc gggctcgtac cgtcgtacgt
    13081 cctcgagcga atgcgggagc gcttggaccc ggccgacgcg tgagcgagcc accctggcac
    13141 ggacagggac gtgtgggtat gaggcaccgg gacgagggag aagaggccac ggtggacgtc
    13201 aagcagctga tcgagcagtt gaggtcgacg atcgagcacg cacgctccat gccgatgtcg
    13261 tcgtcggcgg tcatcaaccg ggccgaggtt ctcgagctca tcgcgtcgtt ggagagcgtc
    13321 ctcccggcag ctttcgagca gtccgaccgc gtgttcgccg agcgggactc cgtgctcgcc
    13381 gaggcggagg cccgcgccgc ccgcatcgtg gaggaagccg gactcgagcg cgaccggctc
    13441 gtctccgaca ccgaggtgta ccggatcgcc gtgcgcgagt cggacagcct gcgctccggc
    13501 gtcgaggacg agtgcgccgc gttgcgcaag gagaccgacg agtacgtcga cacccgcctg
    13561 gcgaacttcg agatcacgtt gacgaagacg ctcgaggcag tctcacgagg tcgcgaccgg
    13621 ctgcaagggc gcaacgagct cgacgaggag agcctcgact cgctcgccgc actcgacgac
    13681 gctggcattc caccactgcc gcagcactga gcgcctcggc gcgaaatccg gccgtgaaca
    13741 ggtggtttgc cgcaggtatc cgcgggtcct cgccgctgcg gacagctggt cgccgatttg
    13801 gggcgggcac ggcatacccg ttaggctgag gcgcctgccc gtgcacgtag ggcagggatc
    13861 cgttgtgccc gtcatcgaag gctccaagcg atgcgtaccg atccgcgttc gccgttcgtg
    13921 atcaacaccc acgaactcgg caggcgccca ggatcgatgc acaccctgcg gttgacgctg
    13981 ccggcgccga cggatctggg tatcgagttg ctcggtgtgc ccccaggctc tccgatcgag
    14041 ctggacctcc ggctcgagtc ggtgatggag ggggtgctcg cgtccggttg ggcgcgagtc
    14101 ctgctcaccg gggagtgcgc acggtgcctg gagccgatcg aggacgacct cgaggtcgag
    14161 ctgcaggagc tgtacgtcta tccggagagc gacgccgagg aggacgaggc gagtcggctc
    14221 gacggcgagc tgctcgacct cgaacccgtg ctccgggact cggtggtgct cgcactgccg
    14281 ttccgacccg tgtgccaacc ggactgtgaa ggtctgtgtc ctgagtgtgg gacccggctg
    14341 ctggacgatc ccggtcacgc gcacgaggag ggcgtcgacc cgcgatgggc ggcgctctcg
    14401 gccctgacaa cgaacgatgc cggtcgcgct gacccgaccg gcaaggacga ggagtaaccg
    14461 tggctgttcc caagcggaag atgtcgcgca gcaacacgcg tcaccgccgt tcccagtgga
    14521 aggctgccgc gccctctctc gtgacgtgtg cgaaccccgc ctgtgggtcc aagcacctgc
    14581 cacaccgggc ctgcccggag tgtggccagt acggcgcccg cgctgaccgc cgacaggtcc
    14641 tctgacggca ggcatgcgtc ccgcggtgga cgacgcagcg ctgggcccgt cacgtgacga
    14701 gctctgcgcc gagctcggag tcgacgacct cgaccccgag ctgctcgacc gtgcgctgac
    14761 gcaccggtcc ttcgcctacg agaacggcgg ccttccgacc aacgagcggc tggagttcct
    14821 cggcgactcg gtgctgggcg tcgtcatcac cgagtcgctc tacctcacgc atcccgacct
    14881 gtccgaagga cgactcgcca agctgcgcgc cgccgtcgtc aacgcccgcg cactggccga
    14941 cgtcgcccgg acgctggggc tgggcgacca catcatgctg ggccgcggcg agatctcgac
    15001 tggtggccgc gacaagtcct cgatcctctc cgacggcgtg gaggccatca tcggcgcgat
    15061 ctacctcgac aaggggttcg atgtcgccgc cggggtgatc caccggctgt tcgacccgtt
    15121 gatggccgcg gcggccgggc tcggcgccgg actcgactgg aagacgagcc tgcaggagct
    15181 gtcggccgac ctgggtcttg gcgttccgga gtacctcatc gacgacagcg gacctgacca
    15241 cgagaagacg ttcgaggcac gggtccgggt cggtgacggc acctacggtc acggcaccgg
    15301 gcggtcgaag aaggaagccg agcagcaggc ggcggagacg gcctggcggg cgatccgtgc
    15361 cgctcacccc gaaaccgggg ggcggagtcc ggcgccgttc gagcccggcg aggccggacc
    15421 gatcgcctcc agcgacccgc acgctccggt ggcgcccggc gagtcggcct gaccggttca
    15481 accgagcgcc cactgtcgtg atccgtgcct gagcttcccg aggtcgaggt cgttcgtcgc
    15541 ggcctccaag ctcacgtcgt cggcacgacg ttcgagcacg tccgggtgct gcatcctcga
    15601 tccgtacgcc ggcacgtcgc cggcatcgcc gacttcgagc acacgctcac cggcagaggt
    15661 gtcgtcggcg tgcgtcgccg cggcaagtac ctctggctcg ctctcgacaa cggggacgcg
    15721 ctgctgggcc atctgggcat gagcgggcag ctgctcgtct tgctgcggac ggccgagacc
    15781 ggcccgcatc tgcgcgtggt gttcaggctc ggcgacgggc gtgatctccg cttcgtggac
    15841 cagcggacgt tcggcgtcct gctcgtgtcc cgtgacggcg cgcagctgcc tgcagagatc
    15901 gcgcacatcg cacgagaccc ggtggacccg ctgttcgacg actcggtctt cgcgtcggct
    15961 gtgcgcaagc ggcgtaccgg gatcaagcgg gcgctactcg accagagcct ggtgtcaggt
    16021 gtcggcaaca tctatgccga cgaggcgttg tgggcggcga ggctgcacta cgcgcgaccg
    16081 accgagacga tgaaccgcac cgagatcgcc cggctgctgg ccgaggtgcg ggcggtcctg
    16141 ctgcgcgcgc tgggggaggg cgggacgtcc ttcgacgcct tgtacgtcaa cgtcgagggc
    16201 gagtcaggct acttcgaccg ttcgctcgcg gtgtacggcc gggaagggcg accgtgtccc
    16261 cgctgcggca cgccgatccg acgcgacacg ttcatgaacc gttcgtcgtt cacctgtccg
    16321 cactgccagc gacgccctcg gcggggtcgc tggtagccga tcccggcgac ccgggctggg
    16381 aggcactctg ccgttcgcgc tggacgctgg gcggcaccga gccgctggag tcgatcggcg
    16441 tccacggctc ggtgtgcacc cactccgagc cacaccctgc acatgcgaac acccggagct
    16501 cctcgccgtt ctcgccgggc cacacctcgc cggtggcgcg ccacacgcag atcgactggc
    16561 agggccacgc ggctaccgca cggcgtacct tcggggcagg tgctggttcg tacctcggca
    16621 tctgcgacat tgtgcccgca ccgggccggc tcaccacgcc tgacgtgcga atccgtggcc
    16681 tccgcgtgtt gaccgcaggg catctgggtg tcacccttga agggagcagc gacggccaac
    16741 accgagtccc cggcgcgtcg gcgagtacct tcgaaacggc gcccgactcg acccggttct
    16801 gcgcatcgcc accgtcgctg atgtcttgat gactggttgc tctggaggac atcgtccatg
    16861 gccaaggctc tccttgggta cgtcggaggg gacatgcgtc tcctcgatga caatcaacgg
    16921 ctccgccgca gaattgctga tctcgaagct gtcgtcctac gccttcaggc ggagaacgac
    16981 gcgctcgcgg ctcgtgtcca cgacggtgaa ctgctgaccg tcccggacag catgcacgag
    17041 cgagccgcag ctcacgcgta gcacgtgatc gacgtccgcc gtctccgccc gcgcgggacg
    17101 gcggggcgga ctgtcggatc gacagcggcg tccacgacgt caccgacgtc gcggcgccgc
    17161 ttttgtgtgc ccaccggtcc ggccgccagg tcggaccgcc tgaacagcac ctcgaacctg
    17221 cccggtgatg tgggcgtttg ccgcgggaac tgcgtaggct cccgccgtga gcttgcacct
    17281 ggtgccgatg accacagcgg agtacgacga gtggcgaggt ggcgccgtct ccggctacgc
    17341 ccaggagttc gtggactcgg ggatcctgca gcccaccgag gccaccgaac gggccgaaaa
    17401 ggacttcgcc gagcttttgc cggacggtct cgcaaccgag aaccacctgt tctggacggc
    17461 gtacgtggac cagagcgatc gggtcgggac gatctgggtc gcattgctgc cggaacggca
    17521 gccaccccac tccttcgtct acgcgttgga cgtccatcct gaccaccgtc gccgcggtta
    17581 cggccaggcc atcatggagg cggccatgca ggagtgccgc gagcgcggca tcgccaccat
    17641 gggcctcaac gtcttcggac acaacgacgt ggcgcgccga ctgtacgacc gactgggttt
    17701 cgtcgtgacc agcacgagca tgaagacgac gctctagtgg cggctgcctg ctgaactccg
    17761 gctcgcccgg ggacgtctgc cggccggaca cgggtgtggt ggtccaagga ttgggtgtgt
    17821 gcggccggta gtctcgctga ctggtcttcc ccacgaccga cgtcaccctg gctgcacaca
    17881 agtccggcgc cggcccaccc agcgacgaca gcgacgacag cgacgacagc gccgcgaagg
    17941 agcaccgcgt tgtacctcaa gagcctgacg ctgcgcggtt tcaagtcctt tgcctcctcc
    18001 acgacgctca agctggagcc cggcatcacc tgcatcgtcg gacccaacgg ctcgggcaag
    18061 tccaacgtcg tggacgcgct ggcatgggtc atgggtgagc agggcgccaa gtcgctgcgg
    18121 ggcggcaaga tggaagacgt catcttcgcc ggcacgtccg gccgtccccc actggggcgc
    18181 gccgaggtac tcctcacgat cgacaacgct gacggcgcat taccgatcga gtacgccgag
    18241 gtgacgatct cgcgcacgat gttccgcaac ggcgggtcgg agtacgccat caacggccag
    18301 acctgccggc tgctcgacat ccaggagctg ctcagcgact ccggtatcgg ccgcgagatg
    18361 cacgtcgtcg tcggtcaggg tcagctcgac cagatcctgc atgcgtcccc ggaggagcgc
    18421 cgcggcttcg tcgaggaggc ggccggcgtc ctgaagcatc gcaagcgcaa ggagaaggcg
    18481 ctgcgcaagc tggacgcgac cgagggcaac ctgacgcgtc tgtccgatct gctgaccgag
    18541 atccgccgcc agctcaagcc gctcggtcgc caggctgaga tcgcccgtcg tgcgtcggtc
    18601 gtccagatgg aggtccgcga cgcacgagcg cgcttgctgg ccgacgatgt ggtgcgggtt
    18661 caggagtcgc tgcaacagga gctcgctgac gaggaggcgc tgcgcggccg gcgtactgag
    18721 gtcgaggcag cgctgacgac gcgccgaacc ggggaagccg agctcgagga ggcgttgcgt
    18781 gccgacgcgc cgctgctcac gaaggcgcag gacacctggt tccgactctc cggcgtacga
    18841 gaacggctgc gtgggacggc gagtctcgcg gccgagcggg tccgcaacct cgcggaggag
    18901 ccggcggagg agcgcgccgg tcgtgatccg gacgagctgg aggccgaggc cgtccaggtc
    18961 agcgcgcggg aggcggagat caccgccgag atcgtcgggc a
//
LOCUS       DATBFZ010000239         7223 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_5329965, whole genome shotgun sequence.
ACCESSION   DATBFZ010000239 DATBFZ010000000
VERSION     DATBFZ010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 7223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7223
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5329965"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..870)
                     /locus_tag="VH419_07270"
     CDS             complement(<1..870)
                     /locus_tag="VH419_07270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043450.1"
                     /translation="MGTVFLHVGTMKSGTSYVQSTLNKNADALAGFGISWHGMQETNL
                     AVQDLTASKLSMPGSKGAWDRLAAKVRATDGDTVISMELLGPCSRRIRHKIAESLGRP
                     QLHLVVTWRDVTKVALSHWQENAQNRQEIGWEQYAEEITAPDRPSGRPHRFWMHHDVA
                     ALLSRWSDVVDPQAMTVITVPSGADQDELWRRFARVIGVPDERARAMPTSRPKNASLG
                     AISAELMLRLNARTRQLGWPQYRLGVKVPLAKRVLSSRAADEPRFALKPEHHKLLRER
                     ALTMVDAVAASPAS"
     gene            complement(1258..3288)
                     /locus_tag="VH419_07275"
     CDS             complement(1258..3288)
                     /locus_tag="VH419_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017933747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEY7043451.1"
                     /translation="MGIADVGKRLLLGRKLRNTQLSETLLPKRIALPVFASDALSSVG
                     YAPDEIFLTLSAAGLVYYSASWKIGICVAFVMAVVIASYRQNVQAYQSGGGDYEVATT
                     NIGSVAGLTVASALLVDYVLTVAVSISSAAQYAAAAIDPLQGHEATIAIIAVLFLMTM
                     NLRGVRESGTAFAIPTYIFMVSILAMSAWGFLRYFHGTLPKSELADYTIQGTMDTAGG
                     VSTAALIFLFLRAFASGSAALTGVEAISNGVPAFRKPKGRNAATTLLLLGTVAVTMAL
                     SILVLGNLMDVRLADVDNGVRLVEPDGTIAPKDFNQDPVIGQLAKGIFDHFPLGFYIV
                     AAATGVILMLAANTAFNGFPVLGSILARDGYLPRQLHSRGDRLAFSNGIVLLAGFAIL
                     LIYVFDAQVTRLIQLYIVGVFVSFTTSQTGMIRHWTRLLRTEQDRAQRGRMIRSRTIN
                     AIGLAVTALVLVIVLITKFTHGAWIAILAMVVLFFLMRGIRHHYDLVAVELAAAEADA
                     SLPSRIHAIVLVSKVHKPTLRAVAYARASRPNFLEAVTVDVDEEVTANLLKQWDDAHL
                     PVPLRVLDSPYREITKPILDYVRDIRRRSPRDVVTVYIPEYVVGRWWEQLLHNQSALR
                     LKGRLLFTPGVMVTSVPFQLHSSAAAERRAERDELLTSNIRRGQARRPPPRS"
     gene            3397..4125
                     /locus_tag="VH419_07280"
     CDS             3397..4125
                     /locus_tag="VH419_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012921371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEY7043452.1"
                     /translation="MSDTGGGCLPHDGEAQPERRGAHVHVVIMGCGRVGSTLARGLER
                     RGHTVAVIDSNPDAFRRLGPNFAGDKVTGVGFDQTVLESAGIKDAGAFAAVSSGDNSN
                     IISARVARETFGLDNVVARIYDPGRAEVYQRLGIPTVATVRWTADQIMRRLLPEGSEP
                     EWRDPSGAIRIEVVDAPEPWVGRKLDELEGLGVRVAFLTRMGEGVMANHETVLQDGDL
                     MHLVMAERDAEKIYAAIEAGPEEH"
     gene            4125..4820
                     /locus_tag="VH419_07285"
     CDS             4125..4820
                     /locus_tag="VH419_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEY7043453.1"
                     /translation="MRVAIAGAGAVGRSVAAELLENGHEVLLIDKDPRAIKADSVPDA
                     EWLLADACELSSLDEAALQRCDVVIASTGDDKANLVISLLAKTEFAVPRTVGRVNHPS
                     NEWLFNESWGVDVSVSTPRIMSALVEEAVTVGDVVRLFTFRQGEANMVELTLPDDSPF
                     VGTPLRDVPWPADSSLVALLREGHVHTPDPDATLEAHDELLFVATSAAEPDLESLLAP
                     EEHNHTAHQPAES"
     gene            4825..5571
                     /locus_tag="VH419_07290"
     CDS             4825..5571
                     /locus_tag="VH419_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043454.1"
                     /translation="MVVSLYTAAERPDLWEAGIDSASVWPEYNLHGDILNQYWGRLDQ
                     ELADFQFVLYDDEEEQVVAEGHTAPFLWDGDDATLPESFDATLLQVFDDLERTASVTA
                     LTALAAETPRDGRRRGLAAGILGAMRVLAKRTGLRHLVAPVRPSWKERYPITPIEEYI
                     GWRRPDGELLDPWMRVHERLGARVGAPMPRSLRITGTVAEWESWTQMAFPATGDYVFP
                     EGLAPVHIDRDADVGSYWEPNVWLIHPEIK"
     gene            complement(5647..6375)
                     /locus_tag="VH419_07295"
     CDS             complement(5647..6375)
                     /locus_tag="VH419_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007077917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3159 domain-containing protein"
                     /protein_id="HEY7043455.1"
                     /translation="MSDSATQPALPPDQHATVEALVRAQLSKALGGKRGMLEGAVPTL
                     GFTITWVSSHDLKLSLIISGGLAVAALLLRIIQRSTIQFVFNSLVGITIAAVFALKSG
                     RAEDAFLPGLIYNTVYAVVLIGSVLVRWPLLGFVIGSVTGDPTAWHKDKRLVSLCSRL
                     TLVLAAPCVVRVIVQYPLWAAGHAGWLGIAKLAMGWPLQLLSLAIMAWMLGRDHTPIR
                     VRAGHPVLEQMEGPLPKSTADADA"
     gene            complement(6417..6794)
                     /locus_tag="VH419_07300"
     CDS             complement(6417..6794)
                     /locus_tag="VH419_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020575710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HEY7043456.1"
                     /translation="MSTEAAPGRLRRALKKLSATPEEIEAGEIDEVREKVGCQSIKAL
                     RDRELVTVYGHLKHVTLAPRAGTPTLEAALYDGSGVVTLVWLGRRTIAGVKPGASLKA
                     HGRVSCQDGRRIIYNPRYELFAP"
BASE COUNT         1240 a         2451 c         2418 g         1114 t
ORIGIN      
        1 agatgcaggg gacgccgcca ccgcgtcgac catcgtgagc gcgcgttcgc gcagcagctt
       61 gtggtgttcg ggcttcagcg cgaagcgcgg ttcatcggct gccctggagc tgagcacccg
      121 cttggccaac ggcaccttca ccccgagccg gtactgcggc caacccagct ggcgcgtccg
      181 agcgttgagg cgaagcatca gctccgccga gatggctccg agcgacgcgt tcttggggcg
      241 actggtcggc atcgcgcggg ctctttcgtc gggcaccccg atgacccgag cgaaccgcct
      301 ccacaactcg tcctgatcgg caccagacgg caccgtgatc accgtcatcg cctgcgggtc
      361 gacgacgtcg gaccacctgc tcagcaacgc ggcgacgtcg tgatgcatcc agaagcggtg
      421 cggcctcccg ctcggccgat ccggcgccgt gatctcttcg gcgtactgct cccaaccgat
      481 ctcctggcgg ttctgcgcgt tctcctgcca gtgagagagg gccaccttcg tcacgtctcg
      541 ccaggtgacg acgaggtgca gctggggacg ccccaacgac tccgcgatct tgtgccggat
      601 ccgcctcgag cacggaccga gcagctccat ggagatgacg gtgtcgccgt cggtggcgcg
      661 caccttggcc gcaagacggt cccatgctcc cttcgacccc ggcatggaga gcttgctcgc
      721 cgtcaggtcc tggaccgcca ggttcgtctc ctgcattccg tgccagctga tcccgaaccc
      781 ggcaagtgca tcggcgttct tgttgagcgt gctctgcacg tacgacgtgc cggacttcat
      841 cgtgccgaca tgcaagaaca ccgtgcccat gcacgcacag agtagtggcc ctcatcgtgt
      901 ctgcaggtcg gccactctcc gagcttccgc cgtgggggtt tgcctcggta gccatccaaa
      961 cggacggcta accaggcagg tctgcctcgg gagggatggg tttgcatggg tagccatgca
     1021 aaccgccgct cgacgacaga ggatgtccga aacccgccag tcacacaccg ggcaaaaggc
     1081 acggaacgcc ggccgaccag ctcaagagac accaacgttc cccgctcttc gctgccgagc
     1141 caccctcagc ccagcacgac agcccgccgg acgaccacgc ctcgtccatc cagaccctct
     1201 cctgccacag gaagcggaac cttcggccga cggcagcatt ggacggacgg catgcgttca
     1261 ggacctcggt ggcgggcgcc gggcctggcc gcggcggatg ttgctggtga gcagctcgtc
     1321 gcgctcggcc cgccgctcgg cggctgccga cgagtgcagc tggaacggca ccgacgtcac
     1381 catcacgccc ggagtgaaca gcagccggcc cttgaggcgc agcgcgctct ggttgtgcag
     1441 cagctgctcc caccaacgtc cgacgacgta ctccgggatg tacaccgtga ccacgtctcg
     1501 cggactgcga cgtcggatgt cgcgcacgta gtcgaggatc ggtttggtga tctcgcggta
     1561 cggcgagtcg agcacgcgca gcggcaccgg taggtgcgcg tcgtcccact gcttgagcag
     1621 gttcgcggtg acctcttcgt cgacgtcgac agtgaccgcc tcgaggaagt tcggacgcga
     1681 tgccctcgcg tacgcgaccg cgcgcagtgt cggcttgtgc accttggata ccagcacgat
     1741 ggcatggatc cgggacggca gcgacgcgtc ggcctcggcg gcggcgagct cgaccgcgac
     1801 caggtcgtag tggtggcgga tcccccgcat caggaagaac agcacgacca tcgccaggat
     1861 cgcgatccag gcgccgtgag tgaacttcgt gatgagcacg atgacgagca cgagcgccgt
     1921 gacagcgagc ccgatcgcgt tgatggtccg gctgcggatc atccggcccc gttgcgcgcg
     1981 gtcctgctcg gtgcgcagca gtcgcgtcca gtgccggatc atgcccgtct ggctggtcgt
     2041 gaacgacacg aagacgccca cgatgtacag ctggatgagt cgtgtcacct gcgcgtcgaa
     2101 cacgtagatg agcaggatcg cgaaccccgc cagcagcacg atgccgttgc tgaacgccag
     2161 ccggtcaccg cgcgagtgaa gctgacgagg gaggtagccg tcgcgggcga gaatcgaccc
     2221 gaggaccggg aagccgttga acgcggtgtt cgccgccagc atgaggatca cgccggtcgc
     2281 agccgccacg atgtagaacc ccagcgggaa gtggtcgaag atgcccttcg cgagctggcc
     2341 gatcaccggg tcctggttga agtccttggg cgcgatcgtg ccgtccggct cgacaaggcg
     2401 tacgccgttg tccacgtcgg cgaggcggac gtccatcagg ttgccgagga cgaggatgga
     2461 cagcgccatc gtgaccgcga ccgtccccag caggagcagc gtcgtcgcgg cgttcctgcc
     2521 cttcggcttc cggaacgccg gcacgccgtt gctgatcgcc tcgacaccgg tcagcgctgc
     2581 agatccggac gcgaacgccc gcaggaacaa gaagatcagc gccgcggtgc tgaccccacc
     2641 agcggtgtcc atcgtcccct ggatcgtgta gtcggcgagc tcggacttcg gcagcgtccc
     2701 gtggaagtag cgcaggaagc cccaggccga catggccagg atcgagacca tgaagatgta
     2761 cgtcggtatg gcgaacgccg tcccggattc gcgcacgcca cgaaggttca tcgtcatcag
     2821 gaacagcacg gcgatgatcg cgatcgtggc ctcgtggcct tgcagggggt cgatcgccgc
     2881 ggcggcgtac tgagctgccg acgagatcga cactgccacc gtcaggacgt agtcgaccag
     2941 cagcgcactg gcgacggtca gcccagccac cgacccgatg ttggtcgttg cgacctcgta
     3001 gtcgccgccg ccggactggt aggcctggac gttctgccga tacgacgcga tcacgaccgc
     3061 catcacgaac gcgacacaga tcccgatctt ccacgaggcg ctgtagtaga cgagcccggc
     3121 cgccgacagc gtcaggaaga tctcgtccgg tgcgtagccc accgatgaga gtgcgtcact
     3181 ggcgaagacc ggaagcgcga tccgcttcgg cagcaacgtc tcgctgagtt gggtgttgcg
     3241 cagcttgcgc ccgaggagca agcgcttgcc gacgtcagca atacccacga ggggagccta
     3301 atgcccacca ccgggatcga agccgctcgc caccggtgtc ggccaagccg tccgcgacgg
     3361 actccgccgg tgtagcgtcg acaactgtcc gagaccttgt cggacactgg tggtggatgc
     3421 cttccgcacg atggggaggc acaacccgaa cgccgaggag cacacgtgca cgtggtgatc
     3481 atgggctgtg gccgggtcgg ctcgacgctg gcccgcggtc tcgagcggcg aggtcacacg
     3541 gtcgcggtca tcgactccaa cccggacgcg ttccggcggc tcgggccgaa cttcgccgga
     3601 gacaaggtga cgggcgtggg cttcgaccag accgtgctgg agtcagccgg catcaaggac
     3661 gccggcgcgt tcgcggcagt cagcagtggc gacaactcga acatcatctc ggcgcgggtc
     3721 gcccgcgaga cattcggtct cgacaacgtc gtggcgcgga tctacgaccc gggtcgggca
     3781 gaggtctatc aacgcctcgg catcccgacc gtggcgacgg tacggtggac cgccgaccag
     3841 atcatgcgtc ggctcctccc cgagggctct gaaccggagt ggcgcgaccc gtccggcgcg
     3901 atccggatcg aagtcgtgga cgctcccgag ccgtgggtcg gacgcaagct cgacgagctc
     3961 gaaggtctcg gcgtacgtgt cgcgttcctc acccggatgg gcgagggcgt catggcgaac
     4021 cacgagacgg tgctgcagga cggtgacctg atgcacctgg tgatggccga gcgggacgcc
     4081 gagaagatct acgccgcgat cgaggccgga ccggaggaac actgatgcgc gtcgccatag
     4141 ccggggccgg ggccgtcggc cgttcggtcg ccgccgagct gctcgagaac ggccacgagg
     4201 tcctgctcat cgacaaggac ccgcgcgcga tcaaggccga cagcgttccc gacgccgaat
     4261 ggctgctggc cgacgcctgc gagctctcct cgctcgacga ggccgcgctg cagcggtgcg
     4321 acgtcgtcat cgcctccacc ggcgacgaca aggccaacct cgtcatctcg ctgctcgcca
     4381 agacggagtt cgcggtgcca cgcaccgtcg gtcgcgtcaa ccacccgtcg aacgagtggc
     4441 tcttcaacga gtcgtggggc gtcgacgtat cggtctcgac tccacggatc atgtctgccc
     4501 tggtggagga ggcagtgacg gttggagacg tcgtccgcct cttcacgttc cgccagggtg
     4561 aggccaacat ggtcgagctg acgttgccgg acgactcacc tttcgtcggc acacctctgc
     4621 gcgacgtacc gtggccggcc gactcgtcac tggtcgcgct gctccgcgaa ggacacgtcc
     4681 acacgccgga cccggacgcg acgctggagg cccatgacga gctgctgttc gtcgcgacca
     4741 gcgccgccga acctgacctc gagtcgctgc tggcccccga agaacacaac cacaccgccc
     4801 accagcccgc cgaatcctga gccggtggtc gtcagcctgt acaccgccgc ggagcggccc
     4861 gacctctggg aagccggcat cgactccgca tcggtgtggc ccgagtacaa cctgcatggc
     4921 gacatcctca accagtactg gggccggctg gaccaggagc tcgccgactt ccagttcgtc
     4981 ctgtacgacg acgaagagga acaggtggtc gccgaagggc acaccgcgcc gttcctctgg
     5041 gacggcgacg acgcgaccct cccggagtcc ttcgacgcga cgctgctgca ggtcttcgac
     5101 gatctcgaga ggaccgcgtc ggtcaccgcc ctgaccgccc tggccgccga gacgccccgt
     5161 gacggacgac gtcgaggcct ggccgccggg atcctcggcg cgatgcgggt cctggcgaag
     5221 cggaccgggc tccgccatct cgtcgcgccg gtccgaccgt cctggaagga gcgctatccg
     5281 atcaccccga tcgaggagta catcggatgg cgacgtccgg acggcgagct gctcgatcct
     5341 tggatgcgag tccacgagcg cctcggcgcg cgggtgggtg ccccgatgcc gaggtcgttg
     5401 cggatcacgg gcaccgtcgc ggagtgggag tcctggacgc agatggcatt cccggccacc
     5461 ggcgactacg tcttccccga aggactcgcg ccggtgcaca tcgatcgaga cgccgacgtc
     5521 ggcagctact gggagccgaa cgtctggctc atccaccccg agatcaagta gtccgtcgag
     5581 cgggttggcg tctggttggc ggctggtcgc ccgcgattgt gggacggcct ccgatgagcc
     5641 gagaggtcag gcgtcggcgt cggcggtgga cttgggaagg ggtccctcca tctgctccaa
     5701 caccgggtgc ccggcgcgta cccggatcgg cgtatggtcg cggccgagca tccaggccat
     5761 gatcgccagc gacagcagct gcaacggcca gcccatcgcg agtttcgcga tcccgagcca
     5821 gccggcgtgg ccggctgccc acaacgggta ctgcacgatg acgcggacca cgcatggcgc
     5881 cgcgagcacc aacgtcaacc gcgaacacaa cgacacgagc cgtttgtcct tgtgccacgc
     5941 ggtcggatcg ccggtgacgc tgccgatcac gaacccgagc agtggccagc gcacgaggac
     6001 cgagccgatg agcacgacgg cgtacaccgt gttgtagatc aagccaggga ggaatgcgtc
     6061 ctccgcacga ccggacttca gcgcgaagac cgccgcgatc gtgatgccga cgagggagtt
     6121 gaagacgaac tggatggtgg accgctggat gatgcgcaac agcagtgcag cgacagcgag
     6181 tcccccggag atgatcaagg acagcttgag gtcgtgcgag ctgacccacg tgatggtgaa
     6241 gcccagggtc gggaccgcac cttcgagcat gccgcgcttg ccaccgagcg ccttcgacag
     6301 ctgggcccgg accagggcct cgaccgttgc gtgctggtcc ggcggcaggg ccggctgggt
     6361 cgcggagtcg ctcattcggt ggctctcgga attcggtcgg ggtggtcggg cagcggtcag
     6421 ggcgcgaaga gttcgtagcg cgggttgtag atgattcgtc gcccgtcctg gcatgagacg
     6481 cggccgtggg ctttgaggct ggccccaggc ttgacaccgg cgatcgtacg acggccgagc
     6541 cagaccaggg tgaccacgcc ggaaccgtcg tacaacgcgg cctccaacgt tggagtcccg
     6601 gcacggggcg cgagcgtgac gtgcttgagg tgaccgtaaa ccgtgaccag ctcgcggtca
     6661 cgcaaggcct tgatcgactg gcagcccacc ttttcgcgga cctcgtcgat ctcgccggcc
     6721 tcgatctcct cgggcgtggc cgacaacttc ttcagcgccc ggcgcaaccg ccccggagcc
     6781 gcttccgtac tcatacctcc agcgtaagcc accgcggcgt gccacccgaa tcgaatgatg
     6841 aaccgacctt ggcgacaacg gtctccggcg gtcgagcctg tcgggatacg gcggtcgagc
     6901 ctgtcgagac cccgggctac ggcggtcgag cctcgagacc ggaggccctc gatctcgaca
     6961 aggtcgatca gcgggtcccc cgacacgcgc gatgagcggc cgtcgagacc cccggttccc
     7021 ggcggtcgag cctgtcgaga ccccgggcta cggcggtcga gcccgtcgag acccccggct
     7081 acatgaaccg cctcgatcag cggttctccc gggctcttga tctcgacaag ctcgatcagc
     7141 gagtccctcg acaagctcga tcagcgggtc cctgacacgc gcgatcagcg ggcagctcga
     7201 tcagcggtcc gtcagagttc gtc
//
LOCUS       DATBFZ010000240        11795 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_5002301, whole genome shotgun sequence.
ACCESSION   DATBFZ010000240 DATBFZ010000000
VERSION     DATBFZ010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 11795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11795
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5002301"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="VH419_07305"
     CDS             <1..498
                     /locus_tag="VH419_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY7043457.1"
                     /translation="WGSCGQLLYVDGQPAGFAMYAPPEYVAMTSPVGGSTVSDDAVLL
                     MTARILPEYGASGLGRVLIQAVVKDLMGRRGVRAIEAFADAQGHEHTCVIPAEFLTAV
                     GFKTVHPHPRYPRLRLDLRSVLSWRGEVEVALERWLGAIRPEKKQRPAGVSPRMNPPG
                     PWPVP"
     gene            complement(630..956)
                     /gene="trxA"
                     /locus_tag="VH419_07310"
     CDS             complement(630..956)
                     /gene="trxA"
                     /locus_tag="VH419_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEY7043458.1"
                     /translation="MSNLSVVTDGDFQAKVLESDKPVLVDFWAEWCGPCRQVDPILDE
                     IAVEHSDKMNFVKMNVDENPVTPANYRVTGIPAINVYKGGELVKSIVGAQPKASILHQ
                     LSDYIG"
     gene            complement(1036..1977)
                     /gene="trxB"
                     /locus_tag="VH419_07315"
     CDS             complement(1036..1977)
                     /gene="trxB"
                     /locus_tag="VH419_07315"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020574631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEY7043459.1"
                     /translation="MTSDVRNVIIIGSGPAGYTAAIYAARADLQPLVFEGSVTAGGAL
                     MTTTDVENFPGFPDGIMGPALMDDLRKQSEKFGAELVPDDIVDVDLTGEIKIVTDTGG
                     NIHRAKTVILAMGSGYRKLGLPNEDALSGHGVSWCATCDGFFFRDQHIAVVGGGDSAV
                     EEATFLTRFASKVTIIHRRDQLRASKIMAHRALSNDKIEFAWNSEVQEVLGDGQLEGV
                     VVKDNQTGQTRTIDVTGLFIAIGHDPRSELVRGQVDLDDAGYVLVDGQSTRTNLDGVF
                     ASGDLVDHTYRQAVTAAGTGCAAALDAERYLTALSDA"
     gene            complement(2151..2960)
                     /locus_tag="VH419_07320"
     CDS             complement(2151..2960)
                     /locus_tag="VH419_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043460.1"
                     /translation="MNARMHPTLDQLAELDEGLLPAAESALVTAHLADCAECRDARAR
                     LGEVSDLLAAAGRETPQMPADVAASVEAALARAGTERDAGVPSLAERRTGSEPDTAAA
                     PAADRPRHRGRMLLGAAAAVTLIAVGGAVISNGLPGGSTPDESAAGTRANDQSAGGES
                     GRAGNVGGRRPTADRVPATVMELSLTPRLNRDSVGSYAERLANGEVAGARPPARCLVT
                     PTTQSQASASVPDALVEFDGQAAVLQVHPDTREFSVFSCPGPARLLYGSTY"
     gene            complement(2957..3802)
                     /gene="sigM"
                     /locus_tag="VH419_07325"
     CDS             complement(2957..3802)
                     /gene="sigM"
                     /locus_tag="VH419_07325"
                     /inference="COORDINATES: protein motif:HMM:NF007225.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigM"
                     /protein_id="HEY7043461.1"
                     /translation="MTVSGRPDVSGIGGPSQRVGRPASLDKHADRDSADSDSRDDAAL
                     LAAHVAGDPEAFSALVRRHRDRLWAVALRTTGDREDAADGLQDGLVAAFRRAETFRGD
                     AQVTTWLHRIVVNACLDRMRRNRSRPTESLPEDEDRATALAIEVGEDPAEAGERRADV
                     MAALATLPDEQRAALVLIDMEGYSVDEAATMLGCAPGTVKSRCSRGRARLVPLLTQYR
                     PAPSADPARSRNHHETGDVQPQTTAASAATQPPGADQQDPPEQRDPADQTERTDPRQH
                     GGSPR"
     gene            complement(3799..5337)
                     /locus_tag="VH419_07330"
     CDS             complement(3799..5337)
                     /locus_tag="VH419_07330"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase family protein"
                     /protein_id="HEY7043462.1"
                     /translation="MGAHLVEPGSVLADRYVIEDLLAEEGDSDSWRARDKVLARSVVL
                     QILPSTSPYAADLVRAAKRASRVADPRILQVLDAIDDGELTYVVREWATGQSLDVILS
                     EGPLPARRATWLMREVAAAMSHAHRMGLPHRRLAPDTVVVTRSSGVKLIGLGTFAAVR
                     PEDDENKPFDAERQDTLDLGRLLYACLTARWPGGATAGLPAAPTEHGRLLRPRQVRAG
                     VPRPLDVVCDRILADPPRYGRPITTVDEVHDALSGILTEEGVTAVGTVGMTPTTNPTP
                     PATEPPPALLLRDESGPVTGDQPAYAGSSPRGASSTLGRTLLLAIVAVLVLGAMLLAY
                     LVAQQGRNGGSAANGVHSQSPSGNAAALKPVTITGGQDFDPPPGSGEENPSEVRNAFD
                     GKPTTTWQTMTYFDPLAEQKPGVGLIVDLGRTQDVSKVDVRLVGSPTDLELRAAPPAA
                     TTAPTGSVDDYSLVQKITDAGTLATFEPAQPVRTRFLLVWLTKLPEETDGFKGQIAEI
                     KAYG"
     gene            complement(5602..7383)
                     /gene="murJ"
                     /locus_tag="VH419_07335"
     CDS             complement(5602..7383)
                     /gene="murJ"
                     /locus_tag="VH419_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011758106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEY7043463.1"
                     /translation="MSDPADNPQDLQAAAHEADAVDPGAAGRMAAADTDATAGSAGNT
                     VEPLTRKVLSATGVMAAGTLISRLTGFVRASLLAAAIGLALNAEVFNVANTIPNSLYI
                     LVAGGVFNTVLVPQLVRAIKNDPDGGDAYANRIITLGALVLAGVTAVLVLTAPLFLRL
                     MVDGRFFTDPALEPQRDALIAFARLCLPQIFFYGMFVLFGQILNARQRFGPMMWTPIA
                     NNLFSIAVIVMYLVVFSEQPATGGYTTGQKLLLGLGSTLGIVLQTVLLIPYLKASGFR
                     IRLRFDLRGTGLGHTLRLGMWMVGFVIVNQLAFYVMVHRATSGPAAITDGVSQSSGFT
                     VYANAFLFTQVPHSIVTVSLATATMTFVSGLAADGRLGDVAHEMASTLRLVLAVIVPF
                     AVALVVFGPALATVLFSWGAAAGDTGPLGNTLIAFAPGMLLFSIHYVVLRGFYALEDT
                     RTPFFIQCLIAAVNIALAITLTAVVDPRYVAPVLALAYGGSYAVGALVSLNVLAHRLG
                     GLDRRGLLGFVGRVALAAAPAVLVSGAVLFGLSGAGLDMSGRSGSLVLLLVGGVLGLA
                     TYLLAARLLRITEITRVVALIAGRLRR"
     gene            complement(7380..9620)
                     /locus_tag="VH419_07340"
     CDS             complement(7380..9620)
                     /locus_tag="VH419_07340"
                     /inference="COORDINATES: protein motif:HMM:NF039636.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6049 family protein"
                     /protein_id="HEY7043464.1"
                     /translation="MVGPLRARVVSRLTRRRLECPILRASVVLAAAVLAVPWSAAAAH
                     QVHRQELDASTTDHGTVPVTAPQVRPRADDTPLERKTGLHVTIDSVEPTTLTPGEPLA
                     VTGAVQDTGRRGWPDAQVYLEITTEPATTRAGLEAFATTTEDVGERIIEFGLFDELGR
                     LKPSETTPYELSVPFQKLPISRAPGVYHVGVVIIATDRDGTRAQVARADTLVPLLPDR
                     GPDGGPLSRTPVVTMIPLAAPVRRVEQNHFLDDDLAAKVAFGGQLRNLLDFVAAAPAD
                     TLEVVLDPALRDAVADMADGYKVRSLAEYENHEPGHDGPGETDAQQWLAELDEAVRHQ
                     DLLFMPWGSPDANALGSHRLLGVVESAVRASQRYASDQRITTAVASWPYAGASTRRGL
                     VASALSGAPLRVVSQNSLVGLRDEDDETDPAYPPSQIVVRSTIGNSTALVTRADVAGR
                     VIETDTPGQEFLRDIVAETSVRSLEGEPGTTVFAAPFGWDPGLAVSPDELVATYGFRT
                     VQPITAARAAQEPAAMYLGRIAMPRGVSQLSIDLMDSIRELGRRGRTYADLLTDHEQT
                     APLFDQVLAAAGTSLWRDDDQARIFANDRNTRVATRELRRVRVTGPTFVALSSASGRF
                     PLTITNGLAVSVTVQVVARAANPAVTIDPIERLVLDPGQRRDIEVTARSKGSGLTGVR
                     VRLMTTTSRPVGRAWNFSIRSTQLGTAIWVVMGVGAAILFTAAARRIYLRVKEGRLHT
                     REEPQR"
     gene            9842..11266
                     /locus_tag="VH419_07345"
     CDS             9842..11266
                     /locus_tag="VH419_07345"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020574637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEY7043465.1"
                     /translation="MQQVTLQTLVAAHPVVPELAERFVAAGHSLALVGGPVRDLLLQR
                     TSTDLDFTTSARPDEIEACLRGWVDAMWDIGRGFGTIGAMRGDDELEITTYRADSYDP
                     ASRKPDVAFGDSLEGDLSRRDFTVNAMALSLPDGAFVDPFGGMGDLATGILRTPGTPE
                     DSFTDDPLRMLRAARFVAQLGLTVVPEVVEAMRTMTERIEIVSAERIRDELVKLLLAA
                     RPRPGLVLLLETGLADRIIPELPALRLERDEHHRHKDVYEHSLTVLDQAIAMEDRLPD
                     GGPDLVTRLAALLHDIGKPRTRRFSDDGSVTFHHHDVVGAKMAAKRLKALRFPSAEVS
                     DVATLVELHLRFHGYGGGEWTDSAVRRYVRDAGPLLDRLHVLTRADCTTRNKRKAERL
                     ARTYDDLEARIARLAEEEELAAMRPDLDGRQIMAVLGIEPGPLVGEAYRYLLERRIDD
                     GPLGAERARAELLTWWEDRQRSLS"
     gene            complement(11300..>11795)
                     /locus_tag="VH419_07350"
     CDS             complement(11300..>11795)
                     /locus_tag="VH419_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012924574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY7043466.1"
                     /translation="SVAILVSGAGFLVAAVITPVATTWLTPPRWIVVLLVVAAAFEIF
                     PGGLFTEAAVLVAAFVLGVSAQGVKICVDTLVQTHVEDVFRGRVFALYDVIFNVVFVA
                     AAAVGAVAVPANGKSYAVLAFIALGYAVTAILYSRTTSGSPRIAGKVRSTPRETPDVS
                     RNSR"
BASE COUNT         1847 a         3949 c         4170 g         1829 t
ORIGIN      
        1 tggggttcct gcgggcagtt gttgtacgtc gacggacaac ctgccggttt cgcgatgtac
       61 gcaccgccgg agtacgtcgc catgacgagc ccggtcggcg gtagcacggt cagcgatgac
      121 gccgtgctgc tcatgaccgc gcggatcctc ccggagtacg gcgcctcggg tctggggcgg
      181 gtactgatcc aagccgtggt caaggatctg atgggtcgac gcggcgtgcg ggcgatcgag
      241 gcgttcgcgg atgcgcaggg gcacgagcac acctgcgtga tcccggcgga gttcctcact
      301 gcggtgggat tcaagacagt ccatccgcac ccccggtacc cgcggttgcg gctggacctt
      361 cgcagcgtgc tcagctggcg aggcgaggtc gaggtggcgc tggagcgctg gttgggtgcg
      421 atccggcccg agaagaagca gcgcccggcc ggggtgtcgc cccggatgaa cccgcctggc
      481 ccctggcctg tcccctagct cgagcgactc ccggcaccgg tggtggtttg cctcggtagc
      541 catccaaacg gacgggtagc caggcagacc tgcctcgcga gcggtgcatt tgcacggcta
      601 gccatgcaaa cccccactcg gcgagcgagt cagccgatgt agtccgagag ctggtggagg
      661 atcgaggcct tgggctgggc gccgacgatg gacttgacca gctcgccgcc cttgtagacg
      721 ttgatggccg gaatgccggt gacacggtag ttcgcgggag tcaccgggtt ctcgtcgacg
      781 ttcatcttga cgaagttcat cttgtcgctg tgctcgacgg cgatctcgtc caggatcggg
      841 tcgacctgac ggcacggtcc acaccactcg gcccagaagt cgaccagcac gggcttgtcg
      901 gactccagca ccttggcctg gaagtcgccg tcggtcacta cggaaaggtt gctcatcaat
      961 gctcctggag ggggtggtag cgggtgctga acgttgcggt ctgagatagc actggtcgtt
     1021 tccgccaagg ctaggtcagg cgtccgacag ggcggtcagg tagcgctcgg cgtccagcgc
     1081 cgccgcacag cccgtgccgg ccgccgtcac cgcctgtcgg tacgtgtggt cgaccaggtc
     1141 accggaggcg aagacaccgt cgaggttcgt cctcgtgctc tgaccgtcca ccaggacgta
     1201 ccccgcatcg tcgaggtcca cctggccgcg tacgagctca gagcgtgggt cgtgcccgat
     1261 ggcgatgaag aggccggtga cgtcgatcgt gcgcgtctgt ccggtctggt tgtccttgac
     1321 cacgactccc tcgagctggc catcgccgag gacctcctgg acctcgctgt tccaggcgaa
     1381 ctcgatcttg tcgttggaca gcgcgcgatg cgccatgatc ttgctcgccc gcagctggtc
     1441 gcgccggtgg atgatcgtca ccttcgacgc gaaccgggtg aggaaggtcg cctcttccac
     1501 cgcagagtca ccgccaccga caacggcgat gtgctggtcg cggaagaaga agccgtcaca
     1561 cgtcgcgcac cagctcacgc catgaccgga caaggcgtcc tcgttgggca gacccagctt
     1621 gcggtagccg gagcccatcg ccaggatgac cgtcttcgct cggtggatgt ttcccccggt
     1681 gtcggtgacg atcttgatct cgccagtgag atccacgtcg acgatgtcgt ccggcacgag
     1741 ttcagcgccg aacttctcgg actgcttgcg caggtcatcc atgagggcag gacccatgat
     1801 gccgtccggg aagccgggga agttctcgac gtcggtggtg gtcatcagcg ctccacctgc
     1861 ggtcacagaa ccttcgaaga cgagcggctg gaggtcggcg cgggccgcgt agatggctgc
     1921 tgtgtagcca gccggtcccg agccgatgat gatgacgttg cggacgtcgg aggtcattgc
     1981 ctgccttgtt cgtgatgctg atgagggtct gctgctgtct gcgttcaacc gatcttaggc
     2041 gttcggcatt cccgtggctg caggcacacg cgccggcgga atgcgggaca acgctccatg
     2101 agtggcccgc gggacgagcc gttgtcccgc acaccgcggc caccgccggg tcagtaggtc
     2161 gagccgtaga gcagccgagc tgggccgggg cagctgaaca ccgagaactc cctcgtgtcc
     2221 gggtggacct gcagcaccgc ggcctggccg tcgaactcga ccagcgcgtc cgggaccgac
     2281 gcggaggcct ggctctgcgt ggtcggggtg acaagacacc gagcgggagg tcgtgcgccg
     2341 gccacttcac cgttcgccag ccgctcggcg tacgagccga ccgagtccct gttcaaccgt
     2401 ggcgtgagcg agagctccat gaccgtcgcg ggaaccctgt ccgcggtcgg tcgacggcca
     2461 ccgacgttgc ctgctcggcc ggattcgccg ccggcggact ggtcgttggc gcgcgtaccg
     2521 gcggcggact cgtcgggagt cgagccgccg ggtagtccgt tcgagatgac cgctccaccc
     2581 accgcgatga gggtgaccgc ggcggctgcc ccgagcagca tgcggccgcg gtggcgcggt
     2641 cggtcagcgg ccggtgcggc cgccgtgtcg ggctcggagc ccgtacgccg ttcggccagc
     2701 gaggggacac ccgcgtcgcg ctcggtgccc gctcgggcca gcgccgcctc gaccgacgcg
     2761 gcaacgtcag cgggcatctg cggtgtctcg cggccggcag ccgcgagcag gtcggacacc
     2821 tcgccgagcc gagccctggc gtcgcgacac tccgcgcagt ccgccaggtg tgcggtgacg
     2881 agcgccgact cggctgcggg cagcaggccc tcgtccagct cggcgagctg gtccagtgtc
     2941 gggtgcatgc gagcgttcac cgtggtgagc ctccatgctg tcggggatcg gttcgttcgg
     3001 tctggtcggc agggtcacgc tgctccggcg ggtcctgctg gtcggcgccc ggcggctggg
     3061 ttgcggcaga cgccgccgtt gtctgaggtt ggacgtcccc ggtctcgtga tggttccgtg
     3121 accttgccgg atccgctgac ggtgcaggcc ggtattgcgt gagcagcggc acgagacgag
     3181 cgcggcctcg cgagcagcgg ctcttcacgg tgccgggagc acaacccagc atcgtggccg
     3241 cctcgtcgac ggagtacccc tccatgtcga tcagcacgag cgcggcgcgt tgctcgtcag
     3301 ggagggtcgc cagcgccgcc atcacgtcgg cccgtcgttc gcccgcctcg gcggggtcct
     3361 cgccgacctc gatggccagc gccgtcgccc ggtcctcgtc ctcgggcagc gactccgtcg
     3421 gacgcgatcg gttccggcgc attcggtcga ggcaggcatt caccacgatg cggtgcagcc
     3481 acgtggtgac ctgggcatca ccgcggaacg tctcggcacg tctgaacgca gccacgagcc
     3541 cgtcctgcag gccgtcggcg gcatcctcgc ggtcacccgt cgtccgcagc gcgacggccc
     3601 acagccggtc ccgatgccgg cgtaccagcg cactgaaggc ctccgggtca ccggcgacgt
     3661 gggcggcgag cagtgcagcg tcgtctcggc tgtcgctgtc ggcgctgtcc ctgtcggcat
     3721 gcttgtccag ggaagcgggg cggccgacgc gttggctcgg gccgccgatc ccggagacgt
     3781 caggtcggcc cgacaccgtc acccgtaggc cttgatctcg gcgatctggc ctttgaaccc
     3841 gtcagtctcc tccggcagct tcgtcagcca gacgagcaga aagcgggtac ggaccggctg
     3901 agccggctcg aacgtcgcca acgtccctgc atcggtgatc ttctgcacca gcgaatagtc
     3961 gtcgaccgac ccggtgggtg cagtcgttgc ggcgggcggt gcagcccgaa gctcgaggtc
     4021 ggtcgggctg ccgacgagcc ggacgtcgac cttcgagacg tcctgcgtcc gtcccaggtc
     4081 gacgatcagc ccgacacctg gcttctgctc ggccaacggg tcgaagtagg tcatcgtctg
     4141 ccacgtcgtc gtcggcttcc cgtcgaaggc gttgcgcacc tcgctggggt tctcctcgcc
     4201 ggaaccgggt ggcgggtcga agtcctggcc tccggtgatc gtcaccggct tgagcgcggc
     4261 ggcgttgccg gacggacttt ggctgtgcac gccgttggcg gcacttccgc cgttcctgcc
     4321 ttgctgggcg acgaggtagg cgagcagcat cgcgccgagc acgagcacgg ccacgatcgc
     4381 cagcagcagc gtccgaccca gcgtggacga cgcgccacga ggagaggatc cggcgtatgc
     4441 cggctggtcc ccggtgaccg gaccgctctc gtcacgcagc agcagcgccg gtggcggctc
     4501 ggtcgcgggc ggagtcgggt tggtggtcgg cgtcatgccg accgtcccga cggcagtgac
     4561 gccctcctcg gtgaggatcc cgctgagcgc gtcgtgcacc tcgtcgaccg tggtgatggg
     4621 gcggccgtag cggggcgggt cagcgaggat ccggtcgcag accacgtcga gtggacgcgg
     4681 cactcccgcc ctgacctggc gggggcgaag cagccggccg tgctcggtcg gggcagcggg
     4741 gaggccggcg gtggcgccgc ctggccaccg ggccgtcaag cacgcgtaca ggaggcgccc
     4801 gaggtcgagg gtgtcttgcc gttcggcgtc gaacggcttg ttctcgtcgt cctcggggcg
     4861 gacggcggcg aaggtcccga gtccgatcag cttcacaccg ctcgacctcg tcaccacgac
     4921 ggtgtcgggg gccagtcggc ggtgcgggag acccatccgg tgcgcatggg acatggccgc
     4981 ggccacctcc cgcatgagcc aggtcgcgcg tcgggcgggg agtggacctt cgctcaagat
     5041 cacgtcgagg gactgcccgg tggcccactc gcggacgacg taggtcagct caccgtcgtc
     5101 gatcgcgtcg agcacctgga ggattcgcgg gtcggccacg cgcgacgccc gcttggccgc
     5161 gcggacgagg tcagcggcgt acggcgaggt cgacggcagg atctggagga cgaccgatcg
     5221 ggcgagaacc ttgtcgcgag cccgccacga atcggagtcc ccctcctcgg cgagcaggtc
     5281 ctcgatgacg taccgatcag cgagcacact gcccggttcg accagatgtg ctcccacgat
     5341 cctcccgtct ccccgtcgtg atgcggttga cacccggtcc ggttcggccg tccaccagcc
     5401 gccgtccggg gagaacggcg gtcgcgactc acaccgtgac caggggcttc cccgtctcat
     5461 ggtaggcggt ccaatgctcc gacaacgtca atccggcagg cgcggacgct gccgctcgtc
     5521 cggcctcccg cgaagggcta gtcgacgccg gcagttgccg ggggcaccgg ccagtcgtcg
     5581 gtgaggtcca ggcggacgag ctcagcggcg caggcgtcca gcgatgagcg cgacgacgcg
     5641 ggtgatctcg gtgatccgca gcagccgtgc cgccagcagg tacgtcgcca ggccgagcac
     5701 accaccgacc agcagcagca ccagggagcc gctccttccg ctcatgtcca gaccggcgcc
     5761 cgagagtccg aacagcacgg ccccggacac gaggacggcc ggcgcagcgg ccagggccac
     5821 gcgcccgacg aagcccaaca gcccgcgtcg gtccaggccg cccagccggt gtgccagcac
     5881 gttgagggag acgagggcgc cgaccgcgta cgacccgccg taggccagcg ccaggaccgg
     5941 cgcgacgtac cgagggtcga ccaccgcggt gagcgtgatc gccaaggcga tgttgaccgc
     6001 ggcgatcagg cactggatga agaacggtgt ccgggtgtct tcgagcgcgt agaagccgcg
     6061 caacacgacg tagtggatgc tgaagagcag catcccgggt gcgaaggcga tcaaggtgtt
     6121 gcccagcggc ccggtgtcgc cggctgcggc tccccagctg aacagcaccg tcgcgagcgc
     6181 cgggccgaac acgaccaagg ccacggcgaa cggcacgatc acggcgagga ccagccgcag
     6241 agtggacgcc atctcgtggg ccacgtcgcc gaggcggcca tcggcagcca gcccggagac
     6301 gaacgtcatc gtggcggtcg ccaacgacac cgtgacgatt gagtgcggga cctgggtgaa
     6361 caagaacgcg ttcgcgtaga cggtgaaacc gctggactgt gagacaccgt cggtgatggc
     6421 tgccgggcct gacgtggccc gatgcaccat gacgtagaac gccagctggt tgacgatgac
     6481 gaagcccacc atccacatcc cgagccgcaa cgtgtggccg aggccggtcc cgcgcaggtc
     6541 gaaccgaagg cggatccgga acccactcgc cttgaggtac gggatcagga gcacggtctg
     6601 caacacgatg ccgagggtgg agccgagccc gagcagcagt ttctggccgg tcgtgtatcc
     6661 acctgttgca ggttgctcgg agaagacgac caagtacatg acgatcactg cgatcgagaa
     6721 caggttgttc gcgatcgggg tccacatcat cggaccgaac cgttgccgag cgttcaggat
     6781 ctgcccgaac aggacaaaca tgccgtagaa gaagatctgc ggaaggcaca gccgggcgaa
     6841 ggcgatgagg gcgtcacgct gtggttcgag tgcgggatcg gtgaagaacc tcccgtcgac
     6901 catcagccgg aggaacagcg gcgccgtcag gaccagtact gcggtgaccc cggccagcac
     6961 cagtgccccg agggtgatga tccggttggc gtacgcgtcg ccgccgtccg ggtcgttctt
     7021 gatcgcacgg accagctgcg ggaccagcac cgtgttgaag acaccacccg cgacgaggat
     7081 gtagaggctg ttcgggatcg tgttcgccac gttgaacacc tcggcgttca acgccagccc
     7141 gatcgcagct gcgagcagcg aagcgcgaac gaatccggtc aaccgcgaga tcagggtgcc
     7201 ggcagccatc acgccggtgg ctgagaggac ctttcgggtg aggggctcga cggtgttccc
     7261 ggccgaaccg gcggtggcat ccgtgtcggc tgcggccatg cgaccagcgg ctccggggtc
     7321 cacggcgtca gcctcgtggg cagcggcctg cagatcctgc ggattgtccg ctgggtcgct
     7381 caccgctgcg gctcctcccg ggtgtgcagc cgtccttcct tcacacgaag gtagatccgg
     7441 cgggcggcgg cggtgaacag gatcgccgcg ccgacaccca tcacgaccca gatcgccgta
     7501 cccagctggg tggaacgaat gctgaagttc caggcacggc cgaccggcct gctcgtcgtc
     7561 gtcatcaggc ggacgcgcac gcccgtgaga ccagacccct tcgaccgcgc ggttacttcg
     7621 atgtcgcgtc gctggccggg atcgagcacg agccgctcga tcgggtcgat ggtcaccgcc
     7681 gggttcgctg cccgggcaac tacctgaacc gtcaccgaga cggcaagccc gttggtgatc
     7741 gtcagcggga agcgacccga tgcgctcgac agcgccacga acgtcgggcc ggtcacacgc
     7801 acccgtcgga gctcgcgggt ggcgacccga gtgttccggt cgttggcgaa gatgcgggcc
     7861 tgatcgtcgt ctcgccacag ggacgtgccc gcggccgcga gaacctggtc gaacaacggg
     7921 gcggtctgct catggtcggt cagcaggtcg gcgtacgtgc gaccccgcct gcccagctcg
     7981 cggatcgagt ccatcaggtc gatggacagc tgcgagacac cgcgaggcat cgcgatccgg
     8041 ccgaggtaca ttgctgccgg ctcctgcgca gcccgagcgg ccgtgatcgg ctgcaccgtg
     8101 cggaacccgt acgtcgccac gagttcgtcc gggctcacgg cgaggcccgg gtcccagccg
     8161 aacggcgccg cgaagaccgt cgttcctggt tcgccctcga gcgagcgcac cgatgtctcg
     8221 gccacgatgt ctcgcaggaa ctcctgacct ggagtgtccg tctcgatgac gcggccggcg
     8281 acgtcggctc gggtcaccag cgcggtgctg ttgccgatcg tgctgcgaac cacgatctgg
     8341 ctgggcgggt acgccgggtc ggtctcatcg tcttcatcac gtagcccgac gagactgttc
     8401 tgcgagacga cgcgaagcgg cgctccggac aaggccgacg caaccaaccc ccgccgggtg
     8461 ctggcaccgg cgtacggcca acttgccacc gcagtcgtga tgcgctggtc cgatgcgtac
     8521 cgctgactgg cccgcacggc cgactcgacg acgccgagca ggcggtgcga cccgagtgcg
     8581 ttcgcatccg ggcttcccca cggcatgaac aacaggtcct ggtgacgcac agcctcgtcg
     8641 agctctgcca gccactgctg tgcgtccgtc tcgccgggac cgtcgtggcc gggttcatgg
     8701 ttctcgtact cggccaggct gcgcaccttg tagccgtccg ccatgtccgc gacggcatcg
     8761 cgcaacgccg ggtcgaggac aacctcgagc gtgtccgccg gcgctgccgc gacgaagtcg
     8821 agcaggttcc tcagctggcc accgaacgca accttggcgg ccaggtcgtc gtcgaggaag
     8881 tggttctgct cgacccggcg gactggcgcg gcgagcggga tcatggtcac gaccggggta
     8941 cgtgacagcg ggccgccgtc cggccctcgg tcggggagaa gcgggaccag cgtgtcggcc
     9001 cgcgcgacct gcgcacgtgt gccatcgcgg tcggtggcga tgatgaccac cccgacgtgg
     9061 tagacgcctg gagcccggct gatggggagc ttctggaacg gaaccgacag ctcgtacggc
     9121 gtcgtctccg acggtttcaa tctgccgagc tcgtcgaaca gcccgaattc gatgatgcgc
     9181 tcaccaacgt cctccgtcgt agtggcgaag gcctcgaggc cggcccgcgt ggtggccggt
     9241 tctgtggtga tctcgagata gacctgcgcg tccggccatc cacgtcggcc ggtgtcctgg
     9301 acagcgccgg tgacagcgag cggttcgccg ggcgtcaagg ttgtcggttc gacggagtcg
     9361 atggtgacgt ggagaccggt cttgcgttcc aacggcgtgt cgtcggcgcg tggacgcacc
     9421 tgcggcgcgg tcaccgggac ggtgccgtga tcggtggtcg acgcgtccag ctcttgccga
     9481 tggacctggt gagcggccgc tgcgctccag ggaacggcga ggacagcagc ggccagcact
     9541 acggacgcgc gcaggatggg gcactcaaga cgtcgtctcg tgaggcggga aacgacgcga
     9601 gctcggaggg gcccgaccat caccggcaga cttcgcacgg cgtacagggg gcctggagca
     9661 tgccgatgat ggtagccgtc gaaggctggg cctcctggag cctggccggg acggcctcgg
     9721 cgcgtgttct ccgtgtcctg gcgaccggtc tgacgccgcg ccggcaaccc tcatacagtg
     9781 gggcatcgtg tcgtctcccc gtgtgcccgc tcctcccggg cgcgaccatc cggcctcgtc
     9841 gctgcagcag gtgacactgc agacgctggt cgctgcacac cctgtcgtgc cggaactggc
     9901 cgagcggttc gtcgcggcag gtcacagctt ggcgttggtc ggtgggccgg tacgggacct
     9961 gctgttgcag cgcacgtcga ccgacctgga cttcacgacg tccgcgcggc cggacgagat
    10021 cgaggcctgc ctgcgcgggt gggttgacgc gatgtgggac atcggccgag gcttcggcac
    10081 catcggcgcg atgcgcggcg atgacgagct ggagatcacc acgtaccgag ccgactcgta
    10141 cgacccggcg tcccggaaac ccgacgtcgc gttcggcgac agcctcgaag gtgacttgtc
    10201 acgtcgcgac ttcaccgtca acgcgatggc gctgtcgctg ccggacggcg cgttcgtcga
    10261 cccgttcggc gggatggggg atcttgccac cgggatccta cgtacgcctg gtacgccgga
    10321 ggactccttc accgacgatc cactccgtat gctgcgggcc gcccgcttcg tcgcgcagct
    10381 cggcctcacg gtggtgcctg aggtcgtcga ggccatgcgg accatgacgg agcggatcga
    10441 gatcgtctcc gcagaacgca ttcgcgacga gctggtgaag ctgctgctcg ccgcgcgacc
    10501 gcgcccgggt ctcgtcctgc tgctcgagac cggactcgcc gaccgcatca tcccggaact
    10561 gcccgcgcta cggctcgaac gagacgagca ccatcgccac aaggacgtct acgagcacag
    10621 cctgacggtg ctcgaccagg cgatcgcgat ggaggatcga ctgcccgacg gtggacccga
    10681 cctggtgacc cggctggcgg cgctgctgca cgacatcggg aagccccgga cgcgcaggtt
    10741 cagcgacgac gggtccgtga cgttccacca ccacgacgtg gtgggcgcga agatggctgc
    10801 caagcgactc aaggcactgc gcttcccgag cgccgaagtc tcggacgtgg cgacgctggt
    10861 cgagctccac ctgcggttcc acggctacgg cggcggtgag tggacggact cagccgtgcg
    10921 ccggtacgtc cgcgatgccg gaccgctgct ggaccggctg cacgtcctga cccgtgccga
    10981 ctgcacgacg cgcaacaagc gcaaggccga gcggctggcg aggacctacg acgacctcga
    11041 ggcgcggatc gccagactcg ccgaggagga ggagctggcg gcgatgcggc cggacctcga
    11101 cggcagacag atcatggcgg tcctcgggat cgaacccggg ccactcgtcg gcgaggccta
    11161 ccggtacctg ctcgagcgac ggatcgatga cggcccactc ggagcggaac gggcccgtgc
    11221 cgagctgctc acctggtggg aggaccgcca acgctccctc tcctgatcac gcccctgccg
    11281 gccgtctgct tcgctgccgc taccgcgaat tgcgggaaac gtctggcgtt tctcggggtg
    11341 ttgaccgcac ttttcccgca attcgcgggg agcctgaggt ggttcgggag tacaggattg
    11401 cggtgacggc gtacccgagg gcgatgaagg cgaggacggc gtacgacttg ccgttggcgg
    11461 gcaccgcgac ggccccgacc gcggcagcgg cgacgaagac gacgttgaag atcacgtcgt
    11521 acagcgcgaa gacccgaccc cgaaagacat cctcgacatg ggtctggaca agcgtgtcga
    11581 cgcagatctt cacgccctgc gccgacaccc cgagcacgaa cgccgcgacg agcaccgcgg
    11641 cttcggtgaa aaggccgccc gggaagatct cgaaggcggc ggcgacgacc agcagcacga
    11701 cgatccagcg tggcggagtc agccaggtgg tcgccaccgg tgtgatgacc gcagcgacca
    11761 agaaccctgc cccggacacg aggatggcga cggac
//
LOCUS       DATBFZ010000241         1544 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_2448732, whole genome shotgun sequence.
ACCESSION   DATBFZ010000241 DATBFZ010000000
VERSION     DATBFZ010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2448732"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..718)
                     /locus_tag="VH419_07355"
     CDS             complement(<1..718)
                     /locus_tag="VH419_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043467.1"
                     /translation="MLQSSDLFLSLFQLTVSVIGSLVVCLGSLYYLRRVRLDRPAIGK
                     FNGRDVAVLFIFLVLLPALYLVVPQWVLTSLLVLTFTAALSIGYRPVLAPGPLWLGIG
                     VLVGADLWMGQHMINNVFNWHFVWAENSVVVALAAVAVANLYVQGGMQLRHVAGFAAA
                     LAVYDAIFTLAFPVSNLLLRAFVNHPLFPAVGMRVGYDEAVLGLGDLLVYGAFTIAAL
                     KAYGRVGLRLALSLVVVFGGI"
     gene            complement(720..>1544)
                     /locus_tag="VH419_07360"
     CDS             complement(720..>1544)
                     /locus_tag="VH419_07360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043468.1"
                     /translation="GIVPPDEALDNLADGGATQLATVPSYLAELEVAARRRGMGPKDF
                     HLRAIDVGGELLSPSLAQAARETFGVTDIVDLFAMTEVVPVVGRTCEQGHLHFDLNTG
                     LVEYLDLETGEPAEPGALATAVITPFFPYRECMPVFRYDTRDVVRPLSDDLTCELAGV
                     PGCSHIEGKADQLIRLGVGEVVTPRQLVDAIEALPTRPWPARFRATVHDGRIRLTLPT
                     SAVAGLDESEVSGHFADRGLDLDLAFVADEESVALRPLRSDLHETTFAGRPALIGA"
BASE COUNT          293 a          505 c          546 g          200 t
ORIGIN      
        1 ggatcccccc gaacacgacg accaggctca atgccagccg caggccaacc cggccgtatg
       61 ccttgagcgc cgcgatggtg aatgccccgt agaccaacag gtcgcccagg ccgaggaccg
      121 cctcgtcata gccgacgcgc atgccgacgg ccgggaacag tgggtggttg acgaacgcgc
      181 gcagcagcag gttcgacacg gggaaggcca aggtgaagat cgcgtcataa acggcgagcg
      241 cggcggcgaa cccggcgacg tgccgcaact gcatgccgcc ctgaacgtag aggttggcga
      301 ccgcgaccgc ggcgagcgcc acgaccacgc tgttctcggc ccacacgaag tgccagttga
      361 agacattgtt gatcatgtgc tgacccatcc agaggtcagc gccgacgagc acgccgatgc
      421 cgagccacaa cggtcccgga gcaaggaccg gccggtagcc gatcgagagt gcggcggtga
      481 aggtcagcac cagcaggctg gtgaggaccc actgcggtac gaccaggtac agcgcgggga
      541 gcagcaccag gaagatgaac aggaccgcga catcgcgacc gttgaacttg ccgatcgctg
      601 ggcggtccag gcgcacccga cgcaggtagt acagcgaccc caggcagacg accagcgagc
      661 cgatgaccga cacggtcaac tggaacaggg acagaaacag gtccgaactc tgcagcatgt
      721 cacgctccga tcagagctgg gcgaccggcg aaggtggtct cgtgcaggtc gctgcgcagc
      781 ggtcgcagcg cgacggactc ctcgtcagcc acgaacgcga gatccaggtc gaggccgcgg
      841 tcggcgaagt gacccgacac ctccgactcg tcgagaccgg cgaccgccga ggtcggcagc
      901 gtgagccgga tgcgtccatc gtggacggtt gcgcggaacc gggccggcca gggacgcgtg
      961 ggcagtgcct cgatggcgtc gacgagctgg cgtggggtca cgacctcgcc gacaccgagc
     1021 cggatgagct ggtcagcctt gccctcgatg tggctgcagc cgggcacgcc ggccagctcg
     1081 caggtgaggt cgtcggacaa cggcctgacg acgtcgcggg tgtcgtaccg gaagaccggc
     1141 atgcattccc ggtacgggaa gaatggtgtg atgacggcgg tggccaaggc acccggctcg
     1201 gccggctcgc cggtctcgag atcgaggtac tcgacgaggc cggtgttgag gtcgaagtgc
     1261 aggtggccct gctcgcacgt gcgtccgacc accggcacga cctcggtcat ggcgaacagg
     1321 tcgacgatgt cggtcacccc gaacgtctcg cgagcagcct gcgccaggct cggtgagagc
     1381 agctcgccac cgacgtcgat tgcccgcagg tggaagtcct tcggtcccat gccgcgccgg
     1441 cgtgccgcca cctcgagctc ggcgaggtag ctcgggacgg tcgccagttg cgtcgcgcca
     1501 ccgtcggcga gattgtccag cgcctcgtcc gggggcacga tgcc
//
LOCUS       DATBFZ010000242         9527 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_1793477, whole genome shotgun sequence.
ACCESSION   DATBFZ010000242 DATBFZ010000000
VERSION     DATBFZ010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 9527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9527
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1793477"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /locus_tag="VH419_07365"
     CDS             <1..473
                     /locus_tag="VH419_07365"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY7043469.1"
                     /translation="PTARVRALRRDDGELLEQIMAAMSAQSRYQRFHAPKSRLTRSER
                     AHLTNVDEEHHIAAIALADDGSPVGVARVLRLGGHSASGEVAVAVVDAWQGRGVGQQL
                     LANIRSRACAMGLQELVAMVLSGTRLVRSLERRGWKVHRRDGTVTTLVTPTCCC"
     gene            572..844
                     /locus_tag="VH419_07370"
     CDS             572..844
                     /locus_tag="VH419_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020384931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043470.1"
                     /translation="MRGDRVEIVIDAGNEVRTYQVVATRNGRRVEINTGRGIVEVVEV
                     TRGGTPVRTARFMANRILALVEHPAGSQEPGQRVIDATEPFDDSTP"
     gene            869..1627
                     /locus_tag="VH419_07375"
     CDS             869..1627
                     /locus_tag="VH419_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043471.1"
                     /translation="MALGDERARRWWVRGRRIGSIERAARFVDDVGFALLFPSDRVLL
                     PSLWEAVAGEDAEPFATGMEKNEQRVWAWKDELPLRRLAWYGNFVAGRGSFLSPRLLR
                     LLYPAYGDVDDHEQLDLSPTAHEVAAALIDGPLPSATLRVLIGDRNRYQRAVVELQRQ
                     LLVTNAGVQEQAAGWPSAVIDLTCRRFDVGNGQDQLEAAGRYLEVVLDASAAELARAF
                     HWPVSEAQGHLETLTRSGRAVEAGHRYVDASVIG"
     gene            complement(1600..3366)
                     /locus_tag="VH419_07380"
     CDS             complement(1600..3366)
                     /locus_tag="VH419_07380"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEY7043472.1"
                     /translation="MIPGPVLVALATVGASTALCGVLIQLRRVWVAGSLLIAVGVLLM
                     TGAVLASNGHSDAGLAVFTAAGSLLFPLALATYPRPAWQPVDALALAGVAGTGLVAVV
                     QWSAASVVGTMALLSSLILIAYVWWRIERGPDRDRWALVWMSVGVAVPGLACGFAVFA
                     APTTTGGVVGLSFLVLVAPAMYVGVARPEFVDVRGVVVHTVVLALAAIAYVAVFMTIA
                     SLIEIVSGDVPIVGALSLVGLAAALTLHPMRVALSGVVDELLFGRRPDPLGAAADVAG
                     HIGDDPVLALRAIREALVLPYAAVLVEGSVLAASGDEVTQTRAFPLELGDGRFGELAV
                     ALRVGDLRLSAGDEHVLRLAAPLLAQTLRANQLATDLQQSREATVSALEEERRRLRRD
                     LHDGLGPRLSGIAFTADAARNSLRSNPDDTEQLLASLRVETVTAIDEIRRLVYAMRPP
                     ALDELGLVPALRQQALTLRTPGGSPLRVTVSAVGLPEGVGAAVEVAAYRIVMEALTNV
                     ARHSGGDTATVSLQVDAGALSIDVRDAGRSNGAWSAGVGIQSMRERAAQLGGTLTAGR
                     QNSGGHVHAVLPLGQPMTDAST"
     gene            complement(3363..4025)
                     /locus_tag="VH419_07385"
     CDS             complement(3363..4025)
                     /locus_tag="VH419_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013861346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY7043473.1"
                     /translation="MSVRIVLADDHPVVRGGLRALLSTIDGMEVVGEAADGAAAVRET
                     QLLHPDVVLMDVRMPGLGGVEATRRIREVAPESAVLMLTMYDDDATVLTAMQAGARGY
                     LLKGAEQDEIVGAIRAVGAGQVIFGPGIAGRVLDYFAAPPVPAAEPFPELTERERAIL
                     DLLASGHRTAAIAERLYLSPKTVSNHLTNVFAKLEVADRTEAILRAREGGLGRDRPSE
                     AP"
     gene            4182..4598
                     /locus_tag="VH419_07390"
     CDS             4182..4598
                     /locus_tag="VH419_07390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043474.1"
                     /translation="MSTARSSTTTQPVSTRQRVGLVLAGVLSATSIPSILFPTPDGEE
                     GPPTVILAVNTALGVIGLVAVIVAWRSGNKPAIRVAAGTLIVNMLTGLPAFFVDVSAA
                     IKLASAVGVLVTVLAVALMFSPPRRSTPVLHGGGAS"
     gene            4595..4915
                     /locus_tag="VH419_07395"
     CDS             4595..4915
                     /locus_tag="VH419_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043475.1"
                     /translation="MIAIVTVLLAFPLGYVVRSRLTANTAYAIAYLWAFVYQSMYLTL
                     DVINESAHTAFEPEKFPLAYGVVTLSIFGVGFGLVSLGHWVRDRRVARRTARRTPEPQ
                     RLPA"
     gene            complement(4998..6458)
                     /locus_tag="VH419_07400"
     CDS             complement(4998..6458)
                     /locus_tag="VH419_07400"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HEY7043476.1"
                     /translation="MTRPGSAYLGIDLGTSGLKLTVVGASGEIVADAEAAYDVEAPQP
                     GFAETDPADWLAALRQARADLARRLDASDTPAVPEAVGVTGQMHGVVLTDDAGKPVRP
                     AILWPDQRAASTLAEWQQLDAVVRGRLANPISAGMAGPMLTWLVEHEPEVVGSAALVR
                     SPKDWLRSQLTGDVVTERSDASATLLWDVVADGWSGDAASVAGVRLEQLPDVADSAAV
                     VGSGDWGPIDAAGHDVPVVAGGADTACALLAVRSAYAAENRDAIVVNVGTGIQLVRTN
                     ADPVPRQEPSSHLYADADGGWYEMLAIQNGGLALSWAQRRLDLDWTAFVAAAQASAVG
                     AADLSFLPFVTGERGAIAPPQPRVGWVGRDTLDTGAQARAAFEALAFTIRRGVELLGS
                     AAADAPLVVSGGGARDPWVRQLVSDVLGRPLTYVPLRSASAVGAAVLAARGVGSALPV
                     AADPVDVLPTTSSALDDAYHRWLDAIAASGRAQLAD"
     gene            complement(6455..7309)
                     /locus_tag="VH419_07405"
     CDS             complement(6455..7309)
                     /locus_tag="VH419_07405"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEY7043477.1"
                     /translation="MTNLLGVHALVWSGTWDEAGARRAISSAGAAGFDLIEVPILDPY
                     GIDTAMTRRLLDEYGMQASCSMGLPAEADVSSEDPAVLARGKELLGRAVEVAQELGAT
                     FLCGVLYSQLGKYPAPITDRSRAHVVEGIQSLSERAGAAGVSVSLEVVNRYESNVVNT
                     AEQMLTLLDDAGVDVGVHLDTYHMNIEEDGFAKPVEQCGQRLGYVHIGESHRGYLGTG
                     TIDFDEFFDALLASGYSGPVTFESFSSAVVHPSLSNQLAVWRNLWNSGDDLAGHARSF
                     IAQRLKLS"
     gene            complement(7306..8157)
                     /locus_tag="VH419_07410"
     CDS             complement(7306..8157)
                     /locus_tag="VH419_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020576197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY7043478.1"
                     /translation="MTTQAVSEKPAVDPSDDALVVEGLGKSFGRVQALREATLSLPRG
                     QITALIGDNGAGKSTLVRCLVGVHSPDAGEVRLDGEQVHFSNPEQGRSAGIETVFQEL
                     ALVEDLTVAQNLFLGREETRGFGPVRLLDRRRMRREALDMVSKLAINVPNVTSRVRRL
                     SGGQRQAVALARAAGWSSKVVILDEPTAALGVQETERVEQLVLSLKEQGATILIISHN
                     FEQVLRLSDQVIVMRAGRTVARRRTAETNGQELVGLVTGAIQDPHHPTHEVTDTQPDT
                     PAAQESS"
     gene            complement(8154..9140)
                     /locus_tag="VH419_07415"
     CDS             complement(8154..9140)
                     /locus_tag="VH419_07415"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY7043479.1"
                     /translation="MTVVETAPASRRTGGGQPDRRRLSELPWPELASTLGLIALAIIF
                     GIANSGYLSQGNVEAILQASAILIVLTVGQTFVIATGGIDLSVASTMTLGAVVLGEFY
                     ANGWNIWLAALAGFIAAAIVGVVNGLVISKGGITDFIVTLGTLSAASGLALIISDGKK
                     TTVIEPALIKLSINGIWIFSWAVIIALGIAVVSHVVLFHTRFGVHVLAIGGSAESSRA
                     LGIKVWLVKTGVYVISAGLAGVGALLLVARVGAAEPAANTSFLLNSVAAVVLGGVSLF
                     GGRATIVAPVMGALLLTALVNGLTRVGLSEFYQPLTVGVVVVLAAFLTRFNR"
     gene            complement(9171..>9527)
                     /locus_tag="VH419_07420"
     CDS             complement(9171..>9527)
                     /locus_tag="VH419_07420"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEY7043480.1"
                     /translation="ILSANKDVAGFFAANDDMALGIARAVADAGRTGEVTIISVDGIE
                     DALKAIQSGDLYATVAQYPYAIGQMGVQACKKATAGDELPSNITAPVAVVTKADADEA
                     LAKFPAPFKPFQNPLA"
BASE COUNT         1529 a         3410 c         3211 g         1377 t
ORIGIN      
        1 cacccactgc ccgtgtccgt gcactgaggc gtgacgacgg cgaactgctc gaacagatca
       61 tggcagcgat gagcgcgcag tcgcgctacc agcggttcca cgccccgaag tcccgtctca
      121 cccggtccga gcgggcccac ctgaccaacg tggacgaaga gcaccacatc gcagcgatcg
      181 ccttggccga cgacggttca ccggtgggcg tggctcgcgt cctgcggctg ggcggccact
      241 cggcttccgg cgaggtggcc gtggcagtcg tcgacgcctg gcagggccgc ggtgtgggac
      301 agcagctctt ggcgaacatc cgctctcgcg cctgtgccat gggcctccag gaactggtcg
      361 ccatggtgtt gagcgggacg agactggtcc gttcgctcga gcggcgtgga tggaaggtgc
      421 accgtcgaga cggcacggtg acgacgctgg tcacacccac ctgctgctgc tgactccccg
      481 ccaattgcgg ggagttccgc gatttcctcg ctccgaatcg cggaacctcc cgcaattcgc
      541 ggggagcggg tcggcggcta ccgtggaggg cgtgcgcggg gaccgggtgg agatcgtgat
      601 cgatgcgggc aacgaggtcc gcacctacca ggtcgtcgcc acccgcaacg gtcggcgggt
      661 ggagatcaac accggtcgcg gcatcgtcga ggtggtcgag gtgactcggg gcggtacgcc
      721 ggtccggacc gcgcgattca tggccaaccg catcctggcc ctggtcgagc atcccgcggg
      781 ttcgcaagag ccgggtcagc gggtcatcga cgccactgaa ccgttcgacg acagcacccc
      841 ctgatcggcg ttgtccactc attccgacgt ggcgctcgga gacgagcgag cccgccgatg
      901 gtgggtgcga ggccgacgga tcggctcgat cgagcgtgcc gcgcgattcg tcgacgacgt
      961 gggcttcgcg ttgctcttcc cctcggatcg ggtgctgctt ccgtcgctct gggaggccgt
     1021 cgcaggcgag gacgcggagc ccttcgcgac cgggatggag aagaacgagc agcgcgtctg
     1081 ggcctggaag gacgagctgc cgctccgacg cctcgcctgg tacggcaact tcgtggccgg
     1141 ccgcggttcc ttcctgtccc cacgtttgtt gcggctgctc tatccggcgt acggcgatgt
     1201 cgacgaccac gagcagctcg acctgtcccc caccgcgcac gaggtcgcgg ccgcactcat
     1261 cgatggcccg ctgccgagcg ccacgctgcg cgtcctgatc ggcgatcgca accgctacca
     1321 gcgcgcggtc gtcgagctgc agcgtcagct gctcgtcacg aatgcaggcg tgcaggagca
     1381 ggccgccggc tggccgtcgg ccgtcatcga cctcacctgt cgcaggttcg acgtggggaa
     1441 cggccaggat cagctcgagg ccgccggccg ctatctcgag gtcgtcctgg acgcctccgc
     1501 cgcagaactg gcgcgcgcgt tccactggcc ggtctccgaa gcgcaaggtc acctcgagac
     1561 gctgacccgg tcaggccgtg ccgtggaagc aggccaccgt tacgtcgacg catcggtcat
     1621 cggctgaccg agcgggagga cggcgtggac gtgtccgccg ctgttctgcc tgccggcggt
     1681 gagtgtgccg ccgagctggg cggcacgctc ccgcatcgac tggataccta cccctgccga
     1741 ccatgcgccg ttcgaccgac cggcgtcccg gacgtcgatg ctgagcgcgc ccgcgtccac
     1801 ctgcagcgag acggtggcgg tgtcgccacc gctgtggcgg gccacgttgg tcaacgcctc
     1861 catcacgatc cggtacgccg ccacctccac cgctgcgcca accccctcgg gcagcccgac
     1921 ggcgctgacg gtcacccgca gggggctgcc gccgggtgtc cgtaaggtga gcgcctgctg
     1981 ccggagcgca ggcaccagtc cgagctcgtc aagcgccgga ggtcgcatcg catagacgag
     2041 tcggcggatc tcgtcgatcg ccgtcacggt ctcgacccgc aacgacgcca ggagctgctc
     2101 ggtgtcgtcc gggttcgacc ggagactgtt gcgcgccgca tctgcggtga acgcgatgcc
     2161 cgacaaccgc ggaccgagcc cgtcgtgcag gtcgcggcgc aaccgacggc gctcctcctc
     2221 cagcgccgag accgttgcct cgcgcgactg ctggaggtcg gtggcgagct ggttggcgcg
     2281 cagggtctgc gccagcagcg gagccgccag ccggagcacg tgctcgtccc cggccgacag
     2341 acgcagatcg cccacccgca gcgccacggc aagctcaccg aagcgtccgt cgcccagctc
     2401 cagcgggaag gcgcgcgtct gggtgacctc atctccggac gccgccagga ccgacccctc
     2461 gaccagcact gcggcgtacg gcagcaccag tgcttcccgg atcgcgcgca acgcgaggac
     2521 cgggtcgtcg ccgatgtggc cggcgacgtc ggccgccgca ccgaggggat ccggccgccg
     2581 gccgaagagg agctcatcca ccacgccgga caacgccacc cgcatcgggt gcaaggtgag
     2641 cgcggccgcg agtcccacca gcgacagcgc accgacgatc ggcacgtctc cgctcacgat
     2701 ctcgatgagc gacgcgatcg tcatgaacac cgcgacgtac gcgatcgcgg cgagggcgag
     2761 gacgacggtg tgcacgacga cgcctcgtac gtcgacgaac tccggcctcg cgacgccgac
     2821 gtacatcgcc ggcgccacca ggaccagaaa cgacaagccg acgactccac ccgtggtcgt
     2881 cggcgcggcg aacacggcga acccgcacgc cagaccagga accgccaccc cgacggacat
     2941 ccagacgagt gcccaccggt cacggtcggg gccgcgctcg atccgccacc agacgtacgc
     3001 gatgaggatc agggacgaca acaacgccat ggtgccgacc acgctcgcgg ccgaccactg
     3061 gaccaccgcg acgagacccg tacccgcgac gcccgccagc gcgagcgcat cgaccggctg
     3121 ccacgccgga cgcggatagg tcgccagcgc caacgggaac agcagtgagc ccgccgccgt
     3181 gaacaccgcc agcccggcat cggaatggcc gttggaggcg agcaccgcgc cggtcatcag
     3241 cagcacaccg acggcgatca gcagcgagcc ggcgacccag acgcgacgca gctggatgag
     3301 gacaccgcac agcgccgtgg atgccccgac cgtcgcgagg gcgacaagca ccggaccggg
     3361 aatcacggtg cctcgctcgg tcgatcccgt ccgaggccgc cttcgcgggc gcgcaggatg
     3421 gcctccgtcc ggtcggccac ctcgagcttc gcgaagacgt tggtcaggtg gttgctgacg
     3481 gtcttgggtg acaggtagag ccgctcggcg atcgccgcgg tccgatggcc ggaggcgagc
     3541 aggtcgagga tcgcgcgctc gcgctcggtc agctcgggga acggctccgc cgccggcacc
     3601 ggtggcgccg cgaagtagtc cagcacacgg ccggcgatgc ccggtccgaa gatcacctgt
     3661 cccgctccca ccgcccggat ggcgccgacg atctcgtcct gctcggcgcc tttcagcaga
     3721 taaccacgtg cgcccgcctg catcgcggtg agcacggtgg cgtcgtcgtc gtacatcgtg
     3781 agcatgagca ccgcggactc gggagcgacc tcccggatgc gcctcgtggc ctccaccccg
     3841 ccgaggcctg gcatgcgcac gtccatgagg acgacgtccg ggtggagcag ctgcgtctcg
     3901 cgcaccgccg ccgcaccgtc ggccgcctca ccgacgacct ccatcccgtc gatcgtcgac
     3961 agcagcgccc gaaggccacc gcgtacgacg ggatggtcgt ccgccaagac gatccgcacc
     4021 gacatgtccc catcatggcc gcgaagtctc ctgatcgacc cgggaagtcc ttcccgagaa
     4081 gtcgggatga cgcgggagca tggaggagac gctgcgtctg tcgacgggaa cgccgccagg
     4141 cccaccgtgg gagcgaccac ttccacgaaa ggagttgcgc gatgagtacc gcaaggtcga
     4201 gtacgacgac gcagccggtg tcgacgcgtc agagggtggg cctggtcctg gccggcgtcc
     4261 tcagcgccac cagcatcccg agcatcctct tccccacacc tgacggtgag gagggcccgc
     4321 cgacggtcat cctggccgtc aacaccgcac tcggcgtcat cgggctcgtc gccgtcatcg
     4381 tcgcctggcg cagcggcaac aagccggcga tccgggttgc cgcgggcacc ttgatcgtca
     4441 acatgctgac cgggctgccg gcgttcttcg tcgacgtctc ggccgcgatc aagctcgcat
     4501 ccgccgtggg cgtcctcgtc accgtgcttg cagtcgcgct gatgttctcg ccacctcggc
     4561 ggtcgactcc ggtgctgcac ggaggcggtg cgtcatgatt gcgatcgtca ccgtgctgct
     4621 ggcgttcccg ctcggctacg tcgttcgatc acggctcacg gccaatacgg cgtacgccat
     4681 cgcctatctg tgggccttcg tctaccagtc catgtacctg acgctggacg tgatcaacga
     4741 gtccgcccac accgccttcg agcccgagaa gttcccgctc gcctacggcg tggtcacctt
     4801 gtcgatcttc ggcgtgggct tcggcctggt cagcctcggc cactgggtcc gcgaccgccg
     4861 agtggcccgg cgcaccgccc gccggacccc ggaaccgcag cgccttcctg cctgaccgtt
     4921 gtgggtttgc gatgtttaca gcccggaagg gctgtgaaca tcgcaaacct gccatgcaaa
     4981 gaggaagttt gcgatggtca gtcggccagc tgcgcccgac cggacgccgc aatggcatcg
     5041 agccatcgat ggtaggcgtc gtcgagtgca gaagacgtcg tcggcagtac gtcgaccggg
     5101 tccgccgcga cgggcagcgc cgacccgacc ccacgtgcag cgaggacggc ggcgccgacg
     5161 gcagaggcac tgcggagcgg cacgtacgtc agcggccggc ccaacacgtc cgagacgagc
     5221 tgacggaccc atggatcgcg agcgccaccc cccgagacga cgagcggtgc gtctgccgcg
     5281 gctgatccca gcagctcgac gcctcggcgg atcgtgaacg cgagcgcctc gaacgccgct
     5341 cgcgcctgcg cgcccgtgtc caacgtgtcg cggccgaccc agcccacgcg cggctggggc
     5401 ggcgcgatgg caccgcgctc ccccgtcacg aacggcagga acgaaaggtc ggccgcaccg
     5461 accgccgacg cctgggcggc cgccacgaaa gcggtccagt cgaggtcgag ccggcgctgc
     5521 gcccaggaca aggcaaggcc gccgttctgg atcgccagca tctcgtacca cccgccgtcg
     5581 gcatcggcgt acaggtgact cgacggctcc tggcgcggaa ctgggtcggc attcgtccgc
     5641 acgagctgga tcccggtgcc gacgttgacg acgattgcgt cgcggttctc ggctgcgtaa
     5701 gccgaacgga ccgcgagcag tgcacaagcg gtgtcggctc ctcccgcgac gacaggtacg
     5761 tcgtgacctg ccgcatcgat gggaccccaa tcgccactcc cgaccacggc agcactgtcc
     5821 gcgacgtccg gaagctgctc gagccggaca ccggcgaccg aggccgcatc gcccgaccag
     5881 ccgtccgcga ccacgtccca gagcagcgtc gccgacgcat cgctgcgctc ggtcaccacg
     5941 tcaccggtga gctgggagcg cagccagtcc ttcggcgacc gcacgagagc ggccgacccg
     6001 acgacctccg gctcgtgctc gacgagccag gtgagcatcg ggccggccat cccggcgctg
     6061 atcgggttcg ccagccgacc gcgtacgacg gcgtcgagct gttgccactc ggccagcgtc
     6121 gaggcagcgc gctggtcggg ccacaggatc gccggccgga cgggcttgcc cgcgtcgtcg
     6181 gtgagcacga caccgtgcat ctgcccagtc accccgactg cctccggcac agcaggagtg
     6241 tcggaggcat cgaggcgacg cgccaggtct gctcgagcct ggcgaagcgc agcgagccag
     6301 tccgcgggat cggtctcggc gaagccgggc tgaggcgcct cgacgtcgta cgccgcctcc
     6361 gcatcggcga cgatctcgcc actcgcaccg accaccgtga gcttgagccc actcgtgccg
     6421 aggtcgatgc cgaggtacgc cgatccgggc cgggtcacga cagcttgagt cgttgcgcga
     6481 tgaacgagcg ggcgtgtcca gccaggtcgt cgccggagtt ccacaggttc cgccacaccg
     6541 caagctggtt gctcagcgac gggtggacga cggccgagct gaagctctcg aacgtcaccg
     6601 gaccggaata cccggacgcc agcaacgcgt cgaagaactc gtcgaagtcg atcgtcccgg
     6661 tccccagata gccgcggtgc gactcgccga tgtggacgta gccgagccgc tgtccgcact
     6721 gctcgaccgg cttcgcgaag ccgtcctcct cgatgttcat gtgatacgtg tcgaggtgga
     6781 cgccgacgtc caccccggca tcgtcgagca gcgtcagcat ctgctcagcg gtgttcacga
     6841 cgttgctctc gtaccggttg accacctcga ggctgacgct gacaccggcg gcaccggccc
     6901 gctcggacaa cgactggatg ccctcgacga cgtgcgcgcg ggagcggtcg gtgatcggtg
     6961 cggggtactt gccgagctgc gagtacagca ccccgcagag gaaggtcgcc ccgagctcct
     7021 gcgccacctc gaccgcccgg ccgagcagct ccttgccccg agcgagcacc gcagggtcct
     7081 cgctcgagac atccgcctcg gccgggaggc ccatcgagca cgacgcctgc atgccgtact
     7141 cgtcgaggag ccgcctcgtc atcgccgtgt cgatcccgta cgggtcgagg atcggcacct
     7201 cgatgagatc gaaaccggcg gcccccgccg acgagatcgc acgacgtgcc cctgcttcgt
     7261 cccaggtgcc gctccagacg agcgcatgca cacccagcag gttcgtcatg aggactcctg
     7321 cgcggcgggc gtgtcgggct gggtgtccgt cacctcgtgc gtcgggtggt gcgggtcctg
     7381 gatcgcgccg gtcacgagac cgacgagctc ctggccgttc gtctccgcag tacgccggcg
     7441 cgccaccgtg cggcccgcgc gcatcacgat cacctggtcg gacaggcgca acacctgctc
     7501 gaagttgtgg ctgatgatca ggatcgtcgc gccctgttcc ttcaacgaca gcacgagctg
     7561 ttcgacccgc tcggtctcct gcacgcccag cgccgcggtc ggctcgtcca ggatcacgac
     7621 cttcgagctc cagccggcgg cgcgggccag tgcgaccgcc tgccgctggc ccccgctcag
     7681 ccgacgtacc cgggaggtca cgttcgggac gttgatggcg agcttgctca ccatgtccaa
     7741 cgcctcgcgg cgcatccgcc gccggtcgag cagcctgacc ggtccgaacc cacgcgtctc
     7801 ctcgcgaccg aggaagaggt tctgcgcgac ggtcaggtcc tcgaccagcg cgagctcctg
     7861 gaacacggtc tcgatgccgg ccgagcgccc ctgctcgggg ttggagaagt gcacctgttc
     7921 tccgtcgagc ctgacctcgc ccgcgtccgg ggagtgcacg ccgacgaggc agcgcaccag
     7981 ggtggacttg ccggcaccgt tgtcgccgat cagtgccgtg atctgtccgc gcggcagact
     8041 gagcgtcgcc tcgcggaggg cctggacacg accgaagctc ttgcccaggc cttcgacgac
     8101 cagcgcgtcg tccgacggat cgacggccgg tttctcgctg acagcttgtg tggtcatcgg
     8161 ttgaacctcg tgaggaaggc ggcgagtacg acgacgacgc cgacggtgag tggctggtag
     8221 aactccgaca gcccgacccg ggtcaggccg ttgacaaggg cggtgagcaa cagcgcgccc
     8281 atcaccggcg cgacgatcgt ggcgcgcccg ccgaagagac tgacgccgcc cagcacgacg
     8341 gccgccaccg agttcagcag gaagctggtg ttggctgccg gctcggccgc accgacacgg
     8401 gcgaccaaca ggagcgcacc gacgccggcc agccccgccg agatgacgta cacgccggtc
     8461 ttcaccagcc agaccttgat gccgagtgcc cgcgagctct cggccgaacc accgatggcc
     8521 agcacatgca cgccgaagcg ggtgtggaac aacaccacgt gcgagaccac ggcgatcccc
     8581 agcgcgatga tgacggccca cgagaagatc cagatgccgt tgatcgagag cttgatcaac
     8641 gccggctcga tcaccgtcgt cttcttcccg tcggagatga tcagcgccag cccggatgcg
     8701 gccgacaggg tccccagcgt gacgatgaag tcggtgatcc cgcccttgct gatgacgagc
     8761 ccgttgacca ctccgacgat ggcggcggcg atgaagccgg cgagcgcggc gagccagatg
     8821 ttccacccgt tcgcgtagaa ctcccccagg accacggcac cgagcgtcat cgtcgacgcc
     8881 accgacaagt cgatgccacc ggtggcgatc acgaacgtct ggccgacggt cagcacgatc
     8941 aggatcgcgg aggcctggag aatcgcctcg acgttgccct ggctgaggta tccggagttg
     9001 gcgatcccga agatgatcgc gagtgcgatg aggccgaggg tcgacgcgag ctcgggccag
     9061 ggcagttccg acagacggcg tctgtccggt tgcccaccac ccgtccgacg gctcgccggc
     9121 gcggtttcca cgacagtcat gatgacgtcc ttcctgttgg gtgcgacagg tcaggccagc
     9181 gggttctgga agggcttgaa cggcgccggg aacttcgcca gcgcctcgtc ggcgtcagcc
     9241 ttggtgacca cggccacggg ggcggtgatg ttgctgggca gctcgtcgcc ggccgtcgcc
     9301 ttcttgcatg cctggacgcc catctgaccg atcgcgtacg ggtactgcgc aacggtggcg
     9361 tacaggtcgc cggactgaat ggccttgagc gcgtcctcga tcccgtcgac gctgatgatc
     9421 gtcacctcac cggtgcgtcc ggcatcggcg accgcgcggg cgatgccgag cgccatgtcg
     9481 tcgttggcgg cgaagaaccc cgcgacgtcc ttgttggcag acaagat
//
LOCUS       DATBFZ010000243         6604 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_815182, whole genome shotgun sequence.
ACCESSION   DATBFZ010000243 DATBFZ010000000
VERSION     DATBFZ010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 6604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6604
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_815182"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1492
                     /gene="acnA"
                     /locus_tag="VH419_07425"
     CDS             <1..1492
                     /gene="acnA"
                     /locus_tag="VH419_07425"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016669970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HEY7043481.1"
                     /translation="STALRASSPAASITDGLEVLVQLVMAAITSCTNTSNPSVMIGAA
                     LLAKNAVDKGLARKPWVKTSLAPGSKVVTDYYERAGLTPYLEKLGFNLVGYGCTTCIG
                     NSGPLIPEVSEAVNESDLAVVSVLSGNRNFEGRINPDVKMNYLASPPLVVAYALAGSM
                     DVNLLDDPLGQDTDGNDVFLHDIWPSPAEVAEVVGSAITAEMFTKGYSDVFAGDERWQ
                     NLPTPEGDTFAWDDESTYVRKPPYFDDMPAEPTPVEDISGARVLAKLGDSVTTDHISP
                     AGAIKKDSPAGQYLADHGVEQRDFNSYGSRRGNHEVMIRGTFANIRLRNQLAPGTEGG
                     FTRNFAGDGDVTTIYDASVAYAEKGTPLVILAGKEYGSGSSRDWAAKGTALLGVKAVI
                     AESYERIHRSNLIGMGVLPLQYPEGENAESLGLTGEETFDITGVTELNPSTGSGQRSG
                     STPPTVKVRAGSAEFDATVRIDTPGEADYFRHGGIMQYVLRHLLNT"
     gene            complement(1589..2239)
                     /locus_tag="VH419_07430"
     CDS             complement(1589..2239)
                     /locus_tag="VH419_07430"
                     /inference="COORDINATES: protein motif:HMM:NF021886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEY7043482.1"
                     /translation="MNSPAEPAPKRRLTTTSYAILGLLSVRSWTTYELAKQVRRSLSW
                     FWPRAERKLYDEPKRLVEAGLATGQETFTGRRRSTVYEITRAGRKDLADWLGQPSVPR
                     STESEAVLKVFFADAGDLDQLRGNLTAIAAESRERLSALAAMAAEPGRFPERRHLNAL
                     TIRFSLEHEEGTLRWLEWALQQVSQWTSTTDPGDWDAEAALDQLVDRVDRLDPRVR"
     gene            2317..3141
                     /locus_tag="VH419_07435"
     CDS             2317..3141
                     /locus_tag="VH419_07435"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY7043483.1"
                     /translation="MTEQMTTQSVVTPAHDDRGAGGPALLFVPGWCGDRAVFDSLVER
                     LQHHRRVVSVDLRGHGDNERDCPDFTAADEVGELIALIERIGLDRVVPVGLSHAGWFA
                     IELRRRLGADRVPGIVLLDWMVLGTPPGFEDALTALQGSQSWTGVRGQLLSMWSSGVD
                     PSTGSGQAAELDRYLLAMADYGFPHWSRAGREIAAAFAAEGSPLAALDGLEPACPTLH
                     VYAQPADEAYLAAQQGAASSRDWFQVRRLDAASHFPMFEVPDEMTRHIEDFVRQLG"
     gene            3301..4254
                     /locus_tag="VH419_07440"
     CDS             3301..4254
                     /locus_tag="VH419_07440"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEY7043484.1"
                     /translation="MTTPNGSTPVSLRVMVEPQQGATYDDQLRVARAAEDLGFDAFIR
                     SDHFYGMGTPGLPGPTESWATLAALARETSRVRLGTLVTSVTFRHPGLLAVVVAQVDQ
                     MSGGRVDFGVGAGWFETEHAAYGVPFPPTRERFDMLEETLAIVTGLWSTPPGETFSYV
                     GQHYRLTDSPALPKPVQPGGPPVILGGKGSRRTPELTARYAAEFNMPFSDVETTRTQF
                     ARIRGACEAAGRDPDGLVLSNALTLCCGRDDAEVAKRAAAVGYEIDELRAEGLAGTPD
                     EVVDQIGQYVELGSRRIYLQILDLADLDHLELVASRVMPQL"
     gene            4359..5075
                     /locus_tag="VH419_07445"
                     /pseudo
     CDS             4359..5075
                     /locus_tag="VH419_07445"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
     gene            complement(5185..5601)
                     /locus_tag="VH419_07450"
     CDS             complement(5185..5601)
                     /locus_tag="VH419_07450"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEY7043485.1"
                     /translation="MAESCLFCAIVAGEVEAALVFETDDVVGFLDHRPVFKGHTLVVP
                     REHIVTLPDLPAPLLTTVFTAAQHLADVMPAALGSQGTFVAMNNVVSQSVPHFHVHVV
                     PRTKGDGLRGFFWPRTKYAAGEEAEYAAKLRAAWTG"
     gene            complement(5690..>6604)
                     /locus_tag="VH419_07455"
     CDS             complement(5690..>6604)
                     /locus_tag="VH419_07455"
                     /inference="COORDINATES: protein motif:HMM:NF020119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase C-terminal
                     non-catalytic domain-containing protein"
                     /protein_id="HEY7043486.1"
                     /translation="TRKMWLSRLGHVDPFDSDRAHWVDTLHRWFDSQLYGIRNGILRE
                     PRVTVEVRPNEWRTSNRWPVATGPTTLHPRSDGTLGRHARKAVAAFTNNPDQEEADAI
                     TAGPNGSRLLYTTAPLAGNARVSGTTVVRVDVRTEVPTGQLGVFLVDYGSDERVLDSG
                     DGARTLSTESCWGESVTYDDACYFDVARRLGTTPLQTLARGWARLNGAGHHKVTVELT
                     PQDNVLTQGHRLGLVVVASSPDWVVTVDPTPSTYTVRLDKTSLRLPGRVAFNRFAPGA
                     VSRVQAIPRRQAVGGALPVHRESTRLPS"
BASE COUNT         1037 a         2255 c         2309 g         1003 t
ORIGIN      
        1 gtcgacggcg ttgcgggcca gcagcccggc ggccagcatc accgacgggt tggaggtgtt
       61 ggtgcagctg gtgatggcgg cgatcacgtc ctgcacgaac acgtcgaacc cgtcggtgat
      121 gatcggcgcc gccctgctcg ccaagaacgc cgtcgacaaa ggcctggcgc gcaagccgtg
      181 ggtgaagacc tcgctcgcgc cagggtcgaa ggtcgtcacc gactactacg agcgggccgg
      241 cctgacgccg tacctggaga agctcgggtt caacctcgtc ggctacggct gcacgacctg
      301 catcggcaac tccggtccgc tcatcccgga ggtgagcgag gcggtcaacg agtccgacct
      361 ggccgtggtg tcggtgctct ccggcaaccg caacttcgag ggccggatca acccggacgt
      421 caagatgaac tacctcgcct cgccgccgct cgtggtggcg tacgcgcttg cgggctcgat
      481 ggacgtcaac ctgctggacg acccgctcgg ccaggacacc gacggcaacg acgtcttcct
      541 gcacgacatc tggccgtccc ccgccgaggt ggcggaggtc gtcggcagcg cgatcaccgc
      601 ggagatgttc acgaagggct actccgacgt gttcgccggc gacgagcgct ggcagaacct
      661 gccgactccc gagggcgaca cgttcgcgtg ggacgacgag tcgacgtacg tgcgcaagcc
      721 gccgtacttc gacgacatgc cggccgagcc gacaccggtc gaggacatca gcggcgcccg
      781 cgtgctcgca aagctcggtg actccgtcac gaccgaccac atctcgccgg ccggcgccat
      841 caagaaggac tcgccggcgg gacagtacct cgccgaccac ggtgtcgagc agcgcgactt
      901 caactcctac ggatcgcggc gcggcaacca cgaggtgatg atccgcggca ccttcgcgaa
      961 catccggctg cgcaaccagc tcgcgccggg caccgagggc ggcttcactc gcaacttcgc
     1021 cggcgatggc gacgtcacga cgatctacga cgcgtcggtg gcgtacgccg agaagggcac
     1081 gccgctggtg atcctggccg gcaaggagta cggatccgga tcgtcgcgcg actgggcggc
     1141 gaagggaaca gcgttgctcg gggtgaaggc ggtgatcgcg gagtcctacg agcggatcca
     1201 ccggtcgaac ctgatcggca tgggtgtcct gccgttgcag taccccgagg gtgagaacgc
     1261 cgagtcgctc ggtctcaccg gcgaggagac gttcgacatc accggcgtca ccgagctcaa
     1321 cccttcgaca ggctcaggac aacgcagcgg ttccaccccg ccgacggtga aggtcagggc
     1381 cgggagcgcg gagttcgacg caacggtgcg gatcgacacg ccgggggagg cggactactt
     1441 ccggcacggc ggcatcatgc agtacgtcct ccgccacctg ctgaacacct gagcgcgcgc
     1501 cacgcgaatt gcgggaatct ccggcttttc gggtcgcgaa atcccggaac ttcccgcatt
     1561 tcgcggcaac cagcagcggc aatcggcgtc atcggacgcg tgggtcgagc cggtctaccc
     1621 gatccacgag ctggtcgagg gcggcttcgg catcccagtc accggggtct gtcgtcgacg
     1681 tccactggct cacctgctgc agcgcccact ccagccagcg cagcgtgccc tcctcgtgct
     1741 cgagggagaa ccggatcgtg agtgcgttga ggtgccggcg ctcggggaag cgcccgggct
     1801 ctgccgccat ggccgccagt gccgacagcc gctcgcggga ctcggcggcg atggcggtga
     1861 ggttcccccg aagctggtcg aggtcacctg cgtcggcgaa gaagaccttg agcaccgcct
     1921 ctgactccgt cgaccgaggg acagacggct gtcccagcca gtcggccagg tcctttcgtc
     1981 ccgctctcgt gatctcgtaa acggtcgagc ggcgacgacc cgtgaacgtc tcctggccgg
     2041 tcgccagtcc ggcctcgacg agtcgcttcg gctcgtcgta cagcttgcgc tcggctcggg
     2101 gccagaacca gctgaggctg cggcgtacct gtttcgccag ctcgtacgtc gtccacgagc
     2161 ggaccgacag cagtccgagg atggcgtagc tcgtcgtcgt gaggcggcgc ttgggggcag
     2221 gttccgcggg cgagttcatg gaaacatctt gacacaccga tactgtcttg tgcactatct
     2281 gattactgca cagggcagta tccacgagga ggcatcatga cagagcagat gacgacgcag
     2341 tccgtcgtca caccggcaca cgacgaccgc ggggccgggg ggccggccct gctcttcgtg
     2401 cccggctggt gtggcgatcg cgccgtcttc gactcgctgg tcgagcgact gcagcaccac
     2461 cgtcgggtcg tgagcgtcga cctgcgcggc cacggcgaca atgagcgcga ctgccccgac
     2521 ttcaccgccg cggacgaggt cggggagctg atcgcgttga tcgaacggat cggtctggac
     2581 cgggtggtcc cggtcgggct gtcgcatgcc ggctggttcg ccatcgagct gcgccgtcga
     2641 ctcggagccg accgtgtccc cggcatcgtg ctgctcgact ggatggtcct cggcacgcca
     2701 cccgggttcg aggacgcgct gacggcgctg cagggttcgc agagctggac cggcgtacgt
     2761 ggtcagctgc tcagcatgtg gtccagcggc gtcgaccctt cgacgggctc aggacaagca
     2821 gccgagctcg accgctacct gctcgccatg gcggactacg gtttcccgca ctggtcgagg
     2881 gcgggacgcg agatcgccgc ggcgttcgcc gccgaaggtt cgccgctcgc tgcgctggac
     2941 ggtctcgagc cggcctgccc gacactgcac gtgtacgccc agcctgccga cgaggcgtac
     3001 ctcgccgccc agcagggtgc tgcgtcgtcg cgcgactggt tccaggtccg tcgtctcgac
     3061 gcagcgagcc acttcccgat gttcgaggtg cccgacgaga tgacccgcca catcgaggac
     3121 ttcgtgcgac agctcggctg acccagctcg aagggcgccg cgaattgcgg gaacctccgg
     3181 ctttccggcg ggccaaatcc cggaactccc cgcaattcgc agcaagccca gcgggctgcc
     3241 agcgtcgaag gaaccgtctc gacgggctgg gccgccggga ggccgtggca gagtggccgg
     3301 atgacgacac ccaacggctc gaccccggtc tcgctgcgcg tgatggtcga gccgcaacaa
     3361 ggggcgacgt acgacgacca gctccgcgtg gcgcgagccg ccgaggacct cggcttcgac
     3421 gcgttcatcc ggtccgacca cttctacggg atgggcacgc cagggctgcc gggccctacc
     3481 gagtcgtggg cgacgctggc agcgctggct cgtgagacca gccgggtccg gctcggcacc
     3541 ctcgtcacct cggtgacgtt ccggcacccg gggctgttgg cggtcgtggt tgcccaggtg
     3601 gaccagatga gcggtggacg ggtcgacttc ggggtcgggg cgggctggtt cgagaccgag
     3661 catgcggcgt acggcgtccc gttcccgcca acgcgcgaac ggttcgacat gctcgaggag
     3721 accctcgcga tcgtcacggg actgtggagc acgccgcctg gtgagacgtt ctcgtacgtc
     3781 gggcagcact accggttgac cgactcgccc gcgctgccga agccggtcca gcctggtggc
     3841 ccgccggtca tcctcggcgg caagggcagt cgacgtacgc cggagctgac cgcgcggtac
     3901 gccgcggagt tcaacatgcc gttctcggac gtggagacga cgcgcaccca gttcgcccgg
     3961 atccgtggtg cctgcgaggc ggcgggtcgc gaccccgacg ggctcgtctt gtcgaacgcg
     4021 ctgaccctgt gctgcggccg ggacgacgcc gaggtcgcca agcgtgcggc ggccgtcggc
     4081 tacgagatcg acgagctgcg tgcggaaggc ctggcgggca ccccggacga ggtcgtcgac
     4141 cagatcggcc agtacgtcga gctgggcagc cggcgcatct acctccagat cctggacctg
     4201 gccgacctcg accacctcga gctcgtcgcg agccgggtca tgccgcagct gtgagtgctc
     4261 gaccggccgg gcagcgcgcc tacacctggg aggcactcgc gacagcgacc ttgcgtcgcc
     4321 agttcccacg catccgtggc cgcggggaag cggccgtggt ggagctcgtc cggcgcgtcg
     4381 ggccgatcca gtcgcaggtg gcccggtcgc ccttcatcac cgtgtcgtcc cggctgcccg
     4441 gcgcgacgta cgacgcgatc agcgcggcgt acgccggcta cgcactggtg cgcggatcca
     4501 acctgcgcgg cacggtgcac accagtgtcc gagagcacca tccactgctc gacgcggtca
     4561 cccgccgcgt catggacaac ggctggcggc gcaacctcgg cttggagcag agcactccgg
     4621 cggacgtcca ggctgggatc gaggcgttcg cgaccggcgt ctggcgaacc ccggacgagc
     4681 tgcgtgcgaa cctggtggcg tggctcgggg accatgacgg ccacgggtcc gccgagcggg
     4741 cacgcacgac tggggtcggc cgggcgttcg cgcacatcca cagtgcactc atccgacgcc
     4801 cgctcgacgc gaccggctgg gagcggcaga cggcgccggt ctaccgggtt gcagccgacg
     4861 tcttggggga gccgcgctcg gacttggtcg acgatcccga ccgcgcgctc atggcgctga
     4921 cgcgtcagca cctggcggcg tacgggccgg ccaaccggcg cgacatcgct tggtggacgg
     4981 gggagaagct gggcaatgtc gaccgcgctc tgacgactct ggcggaagag ctgaccgagc
     5041 ggcctggtcc ggacggtcag acctatcacg accttctcga cgcaccaggg aacggctggc
     5101 ttccgcgaat tgcgggaaag ttgcggtctc cgcgggcgtg tcggcatgac gtttcccgca
     5161 attcgcgggg accaccgacg gcggtcagcc ggtccaggcg gcgcggagct tggcggcgta
     5221 ctccgcttcc tcgcccgcgg cgtacttggt ccgcggccag aagaagccgc gcagcccgtc
     5281 gcccttcgta cgcgggacga cgtgcacgtg gaagtgcggg acggactggc tgaccacgtt
     5341 gttcatcgcc acgaaggtgc cttgtgagcc cagcgccgcc ggcatcacgt cggcgagatg
     5401 ctgcgcggcc gtgaagacgg tggtcagcaa cggagccggc aggtccggca gggtgacgat
     5461 gtgctcgcgc ggcacgacca acgtgtggcc cttgaagacc ggccggtggt cgaggaagcc
     5521 gacgacgtcg tccgtctcga acaccagggc ggcctcgacc tcaccggcga cgatcgcgca
     5581 gaacaagcag gactcggcca ccggcccaac ctacccgggt tcagtgcctg atccgggtcg
     5641 agcaccctcg atctcgacat gctcgataag cgggtccatg tccgctcggt caggaaggaa
     5701 ggcgcgtgct ctcgcgatga accggaagcg cgccgccgac cgcctgccga cgcgggatcg
     5761 cctgaaccct cgagacggcc ccaggagcga acctgttgaa tgccaccctg cctggcagcc
     5821 gcagcgacgt cttgtcgagg cggacggtgt acgtggaggg tgtcgggtcg acggtcacca
     5881 cccagtccgg agacgaagcc acgaccacca gacccagccg gtggccctgc gtgagcacgt
     5941 tgtcctgcgg cgtcagctca accgtcacct tgtggtggcc ggcaccgttc agccgggccc
     6001 agccacgcgc caaggtctgc agcggggtcg tccccagtcg acgggccacg tcgaagtagc
     6061 acgcgtcgtc atacgtcacc gattcgcccc aacacgactc ggtggacagg gtacgggcgc
     6121 cgtcgccgct gtcgagaacg cgctcatcgc tgccgtagtc gacgaggaag acaccgagct
     6181 ggccggtcgg cacctcggtc cgtacgtcga cgcgcacgac cgtcgttccg ctcacccgag
     6241 cgttcccggc cagtggagcc gtggtgtaca gcaggcgcga accgttcggt ccggccgtga
     6301 tggcgtccgc ctcttcctga tcggggttgt tcgtgaaggc agcgacagcc ttcctcgcgt
     6361 gcctgccgag cgtgccgtcc gaacgcgggt ggagcgtcgt cggaccggtc gcgaccggcc
     6421 accggttgct cgtgcgccac tcgttcggac gtacttcgac ggtgactcgg ggctcgcgga
     6481 ggatgccgtt gcggatgccg tacagctggc tgtcgaacca acggtgcaac gtgtcgaccc
     6541 agtgcgctct gtcggagtcg aaggggtcga cgtggccgag ccggctcagc cacatcttgc
     6601 gggt
//
LOCUS       DATBFZ010000244        24653 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_2780238, whole genome shotgun sequence.
ACCESSION   DATBFZ010000244 DATBFZ010000000
VERSION     DATBFZ010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 24653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24653
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2780238"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1719
                     /locus_tag="VH419_07460"
     CDS             <1..1719
                     /locus_tag="VH419_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043487.1"
                     /translation="VLATSRRPLHLQAEHEYAVPPLRLPLDSSAAAAGEAAAVQLFCQ
                     RAGRVRTGFRLTDANAKEIVAICRRLDGLPLAIELTAARSKLLSPAAMLSRLGTTLDA
                     GSAGVDVPERQRSLRATIAWSNDLLSPSLQIFFRRLGVFPVSDDLDAVAAVTGSGGNV
                     LDELDALVDASLARVIEGPDGEPRVTTLQTVRDFALDALAASGELEDIQRRYAEYYVE
                     LAEAMGPLLMTGQQFEAVDRLELEHDHFRAALRWSLRPDEPTPPPQSDAMIGVRMCAA
                     LWWFWSSQGHSLTEEVWARRAADIAPSVDSSAKARVLLGLGIDAIYHGAGQEGLEPGS
                     LVDTAGRAESTPWELDEAMAIYERLGDRAGMSETACTLAQWFQATDVARAHELYEQAV
                     GLVRGQEHAAATASALHHFADLVWWEGDFERAEAMVRESRDLVARMGNERDVVGRTRD
                     LAVMTMDAGDVAGGLSELLALLPDVRRVRQPTLTLSVVGDIALACSALGDTERAARLF
                     GARDQHALAFGWRSWIERAYVLEQLNRLRSALGEEVCDALMAEGRTLTLDEALEEAER
                     ATPPMD"
     gene            complement(1725..2564)
                     /locus_tag="VH419_07465"
     CDS             complement(1725..2564)
                     /locus_tag="VH419_07465"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HEY7043488.1"
                     /translation="MTGTHPMRFRRPTHGRRTVGVALGLAGLAAGAVVASTPSYGSSS
                     APAAAPASSSVRLASDRTVLSSRKIGHSVRGHPIRAYELGSRKADKTVVLIGAMHGDE
                     REGTTVIDGLLKGRPIRGVHIWAVRVDNRDGYLTGHRRNARGVDLNRNFPTRWKRLTG
                     DFDSGPRPASEPETRALMRFLDRVDPGHVVTMHSPLHGIDVYGAKDRPFARRLSREMG
                     LPRKSFNCSGVCHGTLTQWFNRHHDGACVTVEFGDHPKGRYLHGRATRGLLRAIGGRY
                     AYE"
     gene            complement(2561..3142)
                     /locus_tag="VH419_07470"
     CDS             complement(2561..3142)
                     /locus_tag="VH419_07470"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012923070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEY7043489.1"
                     /translation="MSAGTEVGDDGRRRCPWGLTTPEYVAYHDDEWGQPIHDDNALYE
                     RLTLEGFQSGLSWLTILRKRENFRKAFAGFDIDTVAAYDDGDRARLLADAGIVRHPGK
                     IDAAINNARAAAVLGEPLSQLIWRFAPDASPAPVSVSDVPGQTAESKALAKELRQHEF
                     RFVGPTTAYALMQAAGLVNDHLADCWARERIRA"
     gene            complement(3139..3636)
                     /locus_tag="VH419_07475"
     CDS             complement(3139..3636)
                     /locus_tag="VH419_07475"
                     /inference="COORDINATES: protein motif:HMM:TIGR03544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEY7043490.1"
                     /translation="MIWLWVVALVVVIGAAAVLAAGRDTSMAEAYDDRPDRTVPTGRP
                     LTADDLAEVRFSTAVRGYRMDEVDAFIDRLRADLIAREHLDTESAESTGSAESAQAAE
                     SPPLQDRADAGERIRRHGHAGWNAGAEPSEAHKLGLDPAHDAAPPSEATDSTAGSDDT
                     TSASP"
     gene            3729..4583
                     /gene="folP"
                     /locus_tag="VH419_07480"
     CDS             3729..4583
                     /gene="folP"
                     /locus_tag="VH419_07480"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008361535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEY7043491.1"
                     /translation="MLGGRDFGDDDLLVMAIVNRTPDSFYDRGETFDERAALDRVAQV
                     VAEGADVVDIGGVKAGYGVDVDVDEELRRTASFVEAVRSAYPDLVISVDTWRAAVARE
                     LCRCGADLLNDTWAGHDPQVAEVAAEFGVGLVCSHTGGLEPRTDPHRAWYDDVVADVI
                     ATVTRLADRAVAVGVDRHRILVDPTHDFGKNTWHSLEVTRRLDELVATGWPVLVALSN
                     KDFVGETLGLPVEERVLGTLAATAVSAWQGARVFRAHQVAETRQVLDMVASIRGTRPP
                     SRATRGLA"
     gene            complement(4651..4806)
                     /locus_tag="VH419_07485"
     CDS             complement(4651..4806)
                     /locus_tag="VH419_07485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043492.1"
                     /translation="MARTRLPVVLDESASAYDTAFVSAGKRGTNVDLDPADPLKITVG
                     TTASITR"
     gene            complement(4916..5719)
                     /locus_tag="VH419_07490"
     CDS             complement(4916..5719)
                     /locus_tag="VH419_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEY7043493.1"
                     /translation="MTDFQPGRPREQRRGPVVMRRRQVQSSTTDQRLLDSRGPSEWVH
                     TDPWRVLRIQAEFVEGFGALAELGPAISVFGSARSTPGDADYEQAVKLGGALVTAGFA
                     VITGGGPGAMEAANRGASEAEGVSVGLGIELPFEHRLNEWVDIGINFRYFFARKTMFV
                     KYAQGFIALPGGFGTFDEIFEAVTLVQTQKVTSFPIVLLGTTYWSGLVEWIRDVVLAG
                     GKVSDKDLALLQTTDDVDEAVAIMVEARREREATAGPGGQPALPPHRPD"
     gene            complement(5791..6849)
                     /gene="dapE"
                     /locus_tag="VH419_07495"
     CDS             complement(5791..6849)
                     /gene="dapE"
                     /locus_tag="VH419_07495"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020580252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HEY7043494.1"
                     /translation="MPALDPRQDIVDLTAALVDIESVSGHEQSITDAIQAALSGLPWL
                     ELWRHSNSLVARTQLGLPERVVVAGHVDTVPVNANLPSRIEADALHGLGSCDMKGGVA
                     VALQLAATLDSPSRDLTYVFYEAEEVEAELNGLTLIAEVREEILHGDFAVVMEPSNAV
                     IEAGCQGTITVAVTARGSRAHAARSWNGVNAIHAARDILDRLATYEPRRPEIDGLRYH
                     EGLNAVGIHGGVAGNVVPDLCTVRVNHRFAPDRGVEEAETFLRELFNGYDVEVVDSAP
                     GAMPGLHLPAAAAFLEVVGGVANPKFGWTDVARFSAMGIPAVNFGPGNPELAHTQHEF
                     VPLAQLRSCLAQMTAWLS"
     gene            6926..7867
                     /gene="dapD"
                     /locus_tag="VH419_07500"
     CDS             6926..7867
                     /gene="dapD"
                     /locus_tag="VH419_07500"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012923084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HEY7043495.1"
                     /translation="MTDTRAAAWGFGLATYDAKGILLDAWFPEPHLGTPPADYHAPST
                     LAALEGDDAVRDVSQSLLHVVIDDLGAPPRDAAEVWLRLHLLSHRLVVPHAANLDGVF
                     ALLTNVVWTNLGPCAVEDFEATRVRARESGKDITVLGVDKFPRMTDYVVPTGVRIADA
                     DRVRLGAHLAEGTTVMHEGFVNYNAGTLGSAMIEGRISAGVTVGEGTDIGGGASTMGT
                     LSGGGMDIVSIGRRCLLGANAGIGISLGDDCVVAAGCYVTAGTKVTLTDGSVVKAREL
                     SGQPGWQFWQNSVTGAVEAMRREGSGVELNTALHSNA"
     gene            7860..8828
                     /locus_tag="VH419_07505"
     CDS             7860..8828
                     /locus_tag="VH419_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043496.1"
                     /translation="MRRVGRYVGGVVVIGLLVGGGYVAVQQGLGPLPDPDRCTATADG
                     YTVEISPEQAENASIIAAVAERRGLPARAISIAIATAFQESKLQNLDSGDRDSLGLFQ
                     QRPSQGWGTPKQIMDPFYSANAFYDALVKVDGFEDMPITEAAQRVQRSAFPNAYADHE
                     RDGRTIASAMAGYSQAAFTCTTDTPDLSGQDMTSSGLTPRAEVVRRDVEKAFGTMSLG
                     GFAPGGVDSGHMNGSAHYDGRAIDVFFRPISPENTRRGWVLAHYLVANAARLDVAHVI
                     FDGKIWTAGDTSDQGWRVYTPKAAGDVSPATKAILLHRDHVHVDVF"
     gene            complement(8933..10063)
                     /gene="dapC"
                     /locus_tag="VH419_07510"
     CDS             complement(8933..10063)
                     /gene="dapC"
                     /locus_tag="VH419_07510"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013161492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HEY7043497.1"
                     /translation="MSPSAEAAATRSGRAPVVLPDFPWDRLLGFKEQAVAHPDGLVDL
                     SVGTPVDPTPRSVREALRAAADSPGYPVVWGTPTLRAAIIGYLTRRCGSVELDDDCVL
                     PTIGSKELVAALPSQLGVHPGQTVLVPQVAYPTYAVGALLAGADYVASDATVSVGPRD
                     VPLVWLNSPANPTGRILPIEHLRKVVAWARERGAVVAVDECYFECAWETEPVSVLDPA
                     VNGGSLESVLAIHSLSKRSNMAGYRAGFVAGDPEIVRAVREVRRHSGLMVAGPVQAAM
                     AAALDDDAHIEEQRQRYQERRGLLRRALTSAGFRIDHSEGSLYLWATRGQPCLETVAW
                     LADRGILVAPGDFYGPSGTEHVRVALTATDERIEAACRRLST"
     gene            complement(10109..10438)
                     /gene="fdxA"
                     /locus_tag="VH419_07515"
     CDS             complement(10109..10438)
                     /gene="fdxA"
                     /locus_tag="VH419_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005270452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEY7043498.1"
                     /translation="MTYVIAQPCVDLKDLACVDECPVDCIYEGQRMLYIHPDECVDCG
                     ACEPVCPVEAIFYEDDTPEQWKDYYAANVEFFDDLGSPGGAAKLGVIDKDHPFIAALP
                     PQNQEGE"
     gene            10571..11554
                     /locus_tag="VH419_07520"
     CDS             10571..11554
                     /locus_tag="VH419_07520"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY7043499.1"
                     /translation="MPLQRRHVGQRVVVRRLLPGQQGPSGGPAMTDVTGILETWDEGS
                     FAVRREDGDLVTIERDLFVTGKPIPPRASTRLRVNPERLERICEGGWQPIEAQPLGEW
                     TLRAAGGFTGRANSARVGGDPGVATDAALETVADFYTARGLPVLAQVVVGSDWQTAFV
                     ERGWVTARHAGQDPVVQVASVARTHRRAGERASRNTDSLPARLDSTPSPDWLALYGRT
                     AGIDATVVRRVMESGDEVAFARIGDPVLAIGRGVVTGHWLGLQAVEVVSSRRRQGLAT
                     AVVDTLLAWGASHGALSAYLQVLPDNDAALALYAGYGFTTHHAYRYLTPST"
     gene            complement(11867..13516)
                     /locus_tag="VH419_07525"
     CDS             complement(11867..13516)
                     /locus_tag="VH419_07525"
                     /inference="COORDINATES: protein motif:HMM:NF023650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan binding domain-containing protein"
                     /protein_id="HEY7043500.1"
                     /translation="MPKTHDLRFGVVFVVGLLVVFGGLYAVGYAVAGNKLPAGTTIAA
                     VDVGGLTPDEARTVLQDRLAPRVQRPIKATAAGEDFTFQPQRAGLTFDIDATIDKALG
                     GSPWDPRHMLHVLTGGDDLAPVVDVDNHELAARLRQVAEKIELKPVESVVSFRSGRPV
                     VAPGHAGRSLDYQLSGDRLIAALVAGDDQTTLPIKAVQPDVTAIEATRFAETVGRRAV
                     SGPVRIKVADDALTLQPATFATALRAVSRSDGLRLDVDEDRLYERVKPALRRLPHHPV
                     NARIRFRGDRPVVVPATSGVTVTPDDLAKAVLAAAGRPSGERVARAHVQADTPRVSTH
                     DVRMLRVTGIVAAAQLRIRPAVGGTDPTDALRRLNGELIRPGSTFSFLAAVRDGRTPA
                     ASTVATLTYNAAFFAGLDIPERTSARIYTADFPPGMDAAVEPPVTDLVLRNSTPYGVY
                     VRAYVDSPGGRGSPVRVARVEIWSTRYWRVSARSSGRYSVVPPDVVTNSQRGCVPRAG
                     AAGFDVDVTRLLRRDGHRRSEVTHTSYAPLDQVRCTARSRP"
     gene            complement(13592..14038)
                     /locus_tag="VH419_07530"
     CDS             complement(13592..14038)
                     /locus_tag="VH419_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043501.1"
                     /translation="MTSNLDRGGGSQATKRSTRAPQIDVGRPEWLASRPAILVLALVV
                     AVVSFAVSVLSALVSRMAWRPGTVVVPWGLVLGVVASVSIVYLARTYTRVLGFVAAAG
                     WIIGTALVVGGRPEGDYLLAEDALGIGYLVLSSVPVIGMAAAGGPR"
     gene            complement(14045..14968)
                     /gene="mshB"
                     /locus_tag="VH419_07535"
     CDS             complement(14045..14968)
                     /gene="mshB"
                     /locus_tag="VH419_07535"
                     /EC_number="3.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007077080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010125 - mycothiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-1-D-myo-inositol-2-amino-2-deoxy-alpha-
                     D-glucopyranoside deacetylase"
                     /protein_id="HEY7043502.1"
                     /translation="MTAQPLPDRRMLLVHAHPDDETIANGATMAKYASEGAHVTLVTC
                     TLGEEGEVLVPELEHLASHHEDGLGEHRIGELADAMRILGVIDHRFLGGAGRFRDTGM
                     AVDENGHAIPPPKIRDDAFWAADLLEAASLLVEVIREVRPQVLVTYDEKGAYGHPDHI
                     QAHRVAMYGSVLAAAPSFRRELGEPWDVAKVYWNAMSEQRMREGLRRLRASGDLTTFE
                     GMDPEGELPPFVTADELITATIDAMDHVDKKLDAMRAHATQITVDGPFFALSNNIGNQ
                     VWGVESYRLVKGVAGERGVDGHETSLFAGVA"
     gene            complement(15054..16214)
                     /locus_tag="VH419_07540"
     CDS             complement(15054..16214)
                     /locus_tag="VH419_07540"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEY7043503.1"
                     /translation="MAAPPDIEVVAGALLHHGRVLSARRVHPPDVAGGWELPGGKARP
                     DETREAAVVRELREELGCSVAVVGAIEGRVDVKPGYVLTVLLVRLVSGEPVPHEHDAV
                     RWLGPEELGDVEWLPADRPFLPTLRELLLDGERLPGGNVAGAVRVGRTVRRATGPWTE
                     AVHALLDHLRAAGLVQAPQVLGYDGRGREVLTFLPGRVPDVDVEVLDESTLADAMRWL
                     RRFHEAVREFRPAAGLTWRTVHRGLATDEIVCHHDFAPYNVALTRSPDGECLAGVFDW
                     DMSGPGTALQDLAFAAWNWVPLWRDLAPALAAERVEVMASAYGAAITAVDIVDAVQPR
                     IRRSLDVIRAGQMAGDPGMLNLRTVGEPERTTRSLANLAQRIPAIRRELQRS"
     gene            16661..17449
                     /locus_tag="VH419_07545"
     CDS             16661..17449
                     /locus_tag="VH419_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020385607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043504.1"
                     /translation="MSTGVMGPGRVRVQTRTLRQDRWWIAPLTTFIVFGAFVVYATWR
                     AFEGANYYSAPYLSPFYSPCLTNDCVEGSSDFGHPFSWWQLSPALLILIFPLGFRLTC
                     YYYRKAYYRAFWLSPPACAVAEPHGNYTGETRFPLILQNIHRYFWYAAVLVSLVLTYD
                     VVLAFQDEDGDWGHVGLGTLIMLVNIVLIWLYTVSCHSCRHVVGGRLRHFSKHPVRYK
                     AWTLVSRLNKSHAVYAWVSLFSVALTDYYIYLLATGAFDDPRFF"
     gene            17449..19362
                     /locus_tag="VH419_07550"
     CDS             17449..19362
                     /locus_tag="VH419_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HEY7043505.1"
                     /translation="MSTETTHGPDENRHPMTGNLMSSEETSVDGHGFEEHEYDVIVIG
                     AGGAGLRAAIAAFDAGARTAIICKSLLGKAHTVMAEGGIAAAMGNLWPEDNWQVHFRD
                     TMRGGKMLNHWRMAQLHAQEAPERVLELEEWGALFDRTADGLISQRDFGGHRYARLAH
                     VGDRTGLEMIRTLQQRAVALGIDVFMECTITDIFKDPSTGSGQARGGGGRVSGAFGYW
                     RESGRFIVFKAPSIVLATGGIGKSYEVTSNSWEYTGDGHALALRTGASLINMEFVQFH
                     PTGMVWPPSVRGLLVTESVRGDGGVLRNSEGKRFMFDYIPEFFKAETADTEEEGDRWY
                     SDKTNNRRPPELLPRDEVARAINSEIKAGRGSPHGGVFLDIASRRSPEFIKRRLPSMY
                     HQFKELADVDITAEPMEIGPTCHYVMGGVEVDPDTGESLVQGLYAAGEVAGGMHGSNR
                     LGGNSLSDLLVFGRRAGLNAAYAAQRTAANRPTVAEEDVTAAAARAVAPFEEQGENPY
                     TIQQDLQDTMQRLVGIIRSAEELTESLGEIDKLRARAARLSVVGNRQYNPGWHLAIDL
                     QNMLLVSECIARAALERKESRGGHTRDDYPLPDPGWGAQNLVLTLNGEGRIDLEHQPL
                     PVMPDELQSFFDK"
     gene            19376..20149
                     /locus_tag="VH419_07555"
     CDS             19376..20149
                     /locus_tag="VH419_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009075091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HEY7043506.1"
                     /translation="MGYDLKLRVWRGDKTGGELGDYTVPVEAGEVVLDAIHRLQATQA
                     GDLAVRWNCKAGKCGSCSAEVNGTPRLMCMTRLSDFEPSETITVTPMRAFPVIRDLVT
                     DVSFNYEKAKQVPAFAPTPRDPDGKRRMQQSDVERGQEFRKCIECFLCQNTCHVVRDH
                     ENNKEAFAGPRFFLRFAELDMHPLDTNDRRRLAQDAAGIGMCNITKCCTEVCPEGIKI
                     TDNAIIPMKERVVDRKYDPVVWLGSKIFKRSDRASRAEV"
     gene            20331..21437
                     /locus_tag="VH419_07560"
     CDS             20331..21437
                     /locus_tag="VH419_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043507.1"
                     /translation="MVDWWDVYARLGTWLRFGVSGDGIRLHHGRDLGDRVDALVGRLG
                     GRCGLDGVLDDLDRAATVVVPPTNAADYAFRWNDEDFDDPVWWPQGITTSADAGHRPD
                     GSTDTGRKVVVVAWYAKRVNGLTQGVRLSFVDVTDHAHPRYRHVLLVEPTRRRWSRKV
                     GMRPVPVHAGGIVWYGSAILVADTLGGIRTFALDDVCHPPGAWRGHDYVLPQRASYKA
                     MNDRGVRPFRFSFVSLDQTGHQHQLIAGEYGRSGSQRLVRFALEADRAAPVVERGVAR
                     PIELVTDTFTHMQGATMVDGVWYISSSRGPRPGSLWVRRPGDPATEHREALATSPEDL
                     SYWPHTDTLWNCTENPGMRYVYAMPRSQFGTEKR"
     gene            complement(21710..21982)
                     /locus_tag="VH419_07565"
     CDS             complement(21710..21982)
                     /locus_tag="VH419_07565"
                     /inference="COORDINATES: protein motif:HMM:NF028255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HEY7043508.1"
                     /translation="MFSALFWKKTAERAIKSFAQSLAAVLAASGLGLLTARWMTALST
                     AGMVALLSVLTSVASCKLGPEDDPSMVNPAEPAASTPSPPPVTAPA"
     gene            22081..22377
                     /locus_tag="VH419_07570"
     CDS             22081..22377
                     /locus_tag="VH419_07570"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020385602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEY7043509.1"
                     /translation="MADLLTREQIDDALGSTLTDWTLEGDDTIRRDVTADTFAGGIAL
                     VDKVAVVADEMNHHPDIDIRWTTITFRLKTHSAGGLTDNDLQLAAEINRLVRST"
     gene            22425..22670
                     /locus_tag="VH419_07575"
     CDS             22425..22670
                     /locus_tag="VH419_07575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043510.1"
                     /translation="MTLNRMTILISAIFLALAAAIGVAAAAGGQSGGPSHDKLMPIGT
                     MTLLPWDAHELAMYKVTYHPHQMCDDVRHPAHAKSAM"
     gene            22754..24403
                     /locus_tag="VH419_07580"
     CDS             22754..24403
                     /locus_tag="VH419_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020387780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding dehydrogenase"
                     /protein_id="HEY7043511.1"
                     /translation="MDADVIVVGAGLAGLVATAELADVGRSVILLDQEPETNLGGQAF
                     WSFGGLFVVDSPEQRRMGIKDSLDLAWQDWSGTAGFDRDEDHWPQRWAQAYVDFAAGE
                     KRRWLHDQGMRFFPVVGWAERGGYLATGHGNSVPRFHVTWGTGPGVVEPFERRVRAAV
                     SRGRVRLRFRHRVDELVVAAGTPTGVRGAVLEPSAAARGATSSRIVVGEFEYGAQAVV
                     VTSGGIGGDHDLVRRNWPARLGPPPAEMVTGVPASVDGRLLDITAKAGGHVINPDRMW
                     HYVEGISNWDPIWPGHGIRILPGPSSLWFDALGRRLPAPLFPGFDTLGTLAYLRSTGH
                     DHSWFVLTQKIIEKEFALSGSEQNPDLTGKSVRQVLGRVRSGAPGPVEAFKRHGRDFI
                     VADSFAEMVEGMNALTDEPLLDHDDLLRQVEARDREIDNPFTKDLQVMAVRGARGYRG
                     DRLVRVAAPHRILDPKAGPLIAVKLHVLTRKTLGGLETDLDGRVLRAGGEPLPGVYAA
                     GEVAGFGGGGMHGYRALEGTFLGGCLFSGRQAGRAVAAATA"
BASE COUNT         3950 a         8456 c         8403 g         3844 t
ORIGIN      
        1 gtcctggcca cgtcccgcag accccttcac ctgcaggccg agcacgagta cgccgtaccg
       61 ccgctgcgcc tgccgctcga ctcgagtgcg gcagccgcgg gcgaagcagc cgctgtccag
      121 ctcttctgcc agcgagccgg gagggtgcgc acagggttca ggctcaccga cgcgaacgcc
      181 aaagagatcg tggcgatctg ccggcgcctc gacggcctac cgctggccat cgagctcacc
      241 gccgcgcgga gcaagctcct ctcaccggca gcgatgctga gccggctcgg tacgacgctc
      301 gatgccggaa gcgcgggcgt cgacgtgcct gagcggcaac ggtcgctccg cgccacgatc
      361 gcctggagca acgatctgct gagtcccagc ctgcagatct tcttccgccg cctcggcgtc
      421 ttcccggtct cggacgacct cgacgcagtg gccgcggtga caggcagtgg cggcaacgtt
      481 ctcgatgagc tggatgcact cgtcgacgcg tccttggcac gggtcatcga agggccagac
      541 ggcgagccgc gcgtcacgac gctccagacg gtccgcgact tcgccctcga tgcactcgcc
      601 gcttccggag agctcgagga catccagcgc cgctacgcgg agtactacgt cgagctcgcc
      661 gaagccatgg gtccgctgct catgactggc cagcagttcg aggctgtcga ccggctcgag
      721 ctggagcacg accacttccg ggccgctctg cgctggtccc tgcgacccga cgagcccacg
      781 cccccgcctc agtccgacgc catgatcggc gtgcgcatgt gtgccgcgtt gtggtggttc
      841 tggtcgagcc aagggcactc gctcaccgag gaggtgtggg cgcggcgtgc tgcagacatc
      901 gctccgagcg tcgattcatc tgccaaggcc cgggtgctgc tcggactggg tatcgacgcg
      961 atctaccacg gggcagggca agaagggctc gagccaggca gcctggtcga cactgccggc
     1021 cgcgcggaat cgacgccgtg ggagctcgac gaggcgatgg ccatctacga gcggctgggt
     1081 gaccgtgcag gcatgtcgga aaccgcctgc acgctcgcgc agtggttcca ggcgaccgac
     1141 gtcgcgcgcg cgcatgagct ctacgagcag gcggtgggct tggtgcgcgg gcaagaacat
     1201 gcggcggcaa cggcgtcggc actgcaccac ttcgccgatc tcgtgtggtg ggagggcgac
     1261 ttcgaacgcg cggaggcgat ggtccgggag tcgcgcgatc tggtggcccg gatgggcaac
     1321 gaacgagacg tcgtgggccg aacgagggat ctggccgtca tgacgatgga cgccggcgac
     1381 gtcgcaggtg gtctttcgga gctcctcgcc ctactacccg acgtccgccg cgtccgtcag
     1441 ccgacgctga cactgtcggt cgtcggtgac atcgccctgg cctgcagcgc gctcggcgac
     1501 acagagcgag cggctcggct gttcggggcc cgtgatcagc acgcgctcgc cttcggttgg
     1561 cggagctgga tcgaacgcgc ctacgtcctc gagcagctca atcggctacg ttctgcgctc
     1621 ggtgaggagg tgtgcgacgc actcatggcc gagggacgaa cactgaccct cgacgaggcc
     1681 ctcgaggagg ccgagcgtgc cacgccgccc atggactagg gcgttcactc atacgcgtac
     1741 cgcccgccga tcgcacggag cagcccccgt gttgcccgtc cgtgaaggta ccggcccttc
     1801 gggtggtcac cgaactcgac cgtgacacat gcaccgtcgt ggtgccggtt gaaccactgc
     1861 gtcagggtgc cgtgacagac cccggagcag ttgaacgact tccgcggcag gcccatctcg
     1921 cgcgagaggc ggcgcgcgaa cggacgatcc ttggcgccgt acacgtcgat gccgtgcagc
     1981 ggtgagtgca tggtcacgac atggccgggg tcgacccggt cgaggaagcg catcagcgcc
     2041 cgggtctcgg gctccgacgc cggacgcgga ccggagtcga agtcgccggt caggcgcttc
     2101 cacctcgtcg ggaagttgcg gttcagatcg acaccgcgcg cgttgcgccg gtggccggtc
     2161 aggtatccgt cccggttgtc gacgcggaca gcccagatgt ggacaccgcg gatcggacgt
     2221 cccttcagca gtccgtcgat gaccgtggtg ccctcgcgct cgtcaccgtg catggcgccg
     2281 atcagcacga ccgtcttgtc ggccttcctg ctgcccagct cgtacgcccg gatcgggtgg
     2341 ccgcgaacgg agtgcccgat cttgcgtgaa gacaagaccg tgcggtccga cgcgaggcgt
     2401 acggatgacg atgccggtgc tgcagccggt gcggagctcg agccgtacga cggagtcgac
     2461 gccaccaccg cgcccgccgc cagaccagcg agaccaagcg cgacgccaac ggtgcgtcgg
     2521 ccgtgtgttg gtcggcggaa ccgcatcggg tgcgtgccgg tcatgcgcgg atcctttcgc
     2581 gggcccaaca gtcggccagg tggtcgttga ccagtcccgc agcctgcatc agagcgtagg
     2641 ccgtcgtcgg cccgacgaag cggaactcgt gctgacgcag ttccttggcg agcgccttcg
     2701 actccgccgt ctgcccggga acgtcggaca cgctgaccgg cgcaggactt gcgtctggag
     2761 cgaaccgcca gatcagctgg gacagcggtt caccgagcac tgctgccgcc cgcgcgttgt
     2821 tgatggctgc gtcgatcttg cctggatgtc gaacgatgcc ggcatcggcg agcagccttg
     2881 cccggtcgcc gtcgtcgtac gccgcaacgg tgtcgatgtc gaagccggcg aacgccttcc
     2941 tgaagttctc ccgcttgcgc aggatcgtca gccacgacaa gccggactgg aagccctcga
     3001 gtgtgaggcg ctcgtacagc gcgttgtcgt catggatcgg ctgaccccac tcatcgtcgt
     3061 ggtaggcgac gtactcgggg gtcgtgagcc cccacggaca gcgccgtcga ccgtcgtctc
     3121 cgacttcagt cccggcgctc acggactggc ggatgtcgtg tcgtcggagc cggctgtgga
     3181 gtccgtggct tcggagggcg gggcagcgtc gtgagcaggg tcgaggccga gcttgtgcgc
     3241 ctcgctcggc tcggcgcctg cgttccaccc cgcgtgccca tggcgtcgga tccgctcgcc
     3301 ggcgtcggcc cggtcctgca gcggtggaga ctcggctgct tgggcggact cggcggaccc
     3361 ggtggactcg gcggactcgg tgtcgaggtg ttcgcgagcg atcaggtcgg ctcggagccg
     3421 gtcgatgaag gcgtccacct cgtccatccg atatccgcgc accgcggtgc tgaacctgac
     3481 ctccgcgagg tcgtccgcgg tgagcggtcg gccggtggga acggtgcggt ccgggcggtc
     3541 gtcgtacgcc tcggccatcg acgtgtcacg acccgccgcc aacacggcgg cagcgccgat
     3601 caccacgacg agcgcgacga cccacaacca gatcacggca tcgatcgtgc cacgcttggc
     3661 cctggcggcc ccggctcggg gtcggccgga ccaggaaacc gcgcgagcgg aggtgaggtg
     3721 tgccgctgat gctcgggggc cgcgacttcg gtgacgacga cctcctggtg atggcaatcg
     3781 tcaaccggac gccggactcg ttctacgacc gcggcgagac gttcgacgaa cgtgcggcgc
     3841 tggaccgggt cgcgcaggtc gtggcggaag gcgctgacgt cgtggacatc ggcggcgtca
     3901 aggccggata cggcgtcgac gtcgacgtcg acgaggagct gcgtcggacg gcgtcgttcg
     3961 tcgaggcggt ccgctcggcg tatcccgacc tggtcatcag tgtcgacacc tggcgcgccg
     4021 cggtggcccg ggagctgtgc cggtgtggtg ccgatctgct gaacgacacg tgggcggggc
     4081 acgacccaca ggtggccgag gtggcggcgg agttcggcgt cggcctggtg tgcagccaca
     4141 ccggtgggct ggagccccgg acggatccgc atcgagcctg gtacgacgac gtggtcgccg
     4201 acgtcatcgc gaccgtcacg cggctggcgg accgcgcggt cgcggtgggc gtggatcggc
     4261 accggattct cgtcgacccg acccacgatt tcggcaagaa cacctggcac tcgctggagg
     4321 tcacgcgacg gctggacgag ctggtcgcga cggggtggcc ggtgctggtg gcgttgtcca
     4381 acaaggactt cgtcggcgag acgctcgggc ttccggtcga ggagagggtg ctgggaacgc
     4441 ttgcggcgac cgcggtgtcc gcatggcagg gagcccgcgt gttccgagca caccaggtgg
     4501 ccgagacccg ccaggtcctc gacatggtcg cctccatccg cggcacccgt ccaccttccc
     4561 gcgccacccg cggcctcgcc tgaccccctc cgttgaaccg tgacttgtgg ttcgttcaac
     4621 cgtgacttgt ggttccgcga acgccatgtt tcatcgcgtg atcgatgcgg tggtgccgac
     4681 ggtgatcttc agtgggtctg ccgggtcgag gtcgacgttc gtaccgcgct tgccggccga
     4741 gacgaatgcc gtgtcgtacg ccgacgcgct ttcgtcgagc acgaccggca gtcgcgtacg
     4801 ggccaagggc gagattccgc cgactacgga cctactgatc cgttcacgac aacccacgat
     4861 tcgtgcaacc acaacccacg attcgtgcaa ccacaaacca cggttcaacg ggagatcagt
     4921 cggggcggtg gggagggagg gcgggttggc cgccgggacc ggcggtggcc tcacgctccc
     4981 gtcgcgcctc gaccatgatc gccacggcct cgtcgacgtc gtcggtggtc tgcagcaagg
     5041 cgaggtcctt gtcgctgacc ttcccaccgg ccagtaccac gtcgcggatc cactccacga
     5101 gaccactcca gtacgtcgta ccgagcaaca cgatcgggaa ggacgtcacc ttctgcgtct
     5161 ggaccaacgt cacggcctcg aagatctcgt cgaacgtccc gaacccgccg ggcaaggcga
     5221 tgaagccctg cgcgtacttc acgaacatcg tcttgcgggc gaagaagtag cggaagttga
     5281 tcccgatgtc gacccactcg ttcagccggt gctcgaacgg cagctcgatg ccgagaccga
     5341 ccgacacgcc ttcagcctcg gacgctccac ggttcgcggc ctccatcgct cccggcccgc
     5401 caccggtgat caccgcgaaa ccggccgtga cgagcgcgcc gccgagcttg accgcctgtt
     5461 catagtcggc gtctccaggc gtgctacggg ccgaaccgaa caccgagatg gcgggtccga
     5521 gctcggccag cgcgccgaag ccttcgacga actccgcctg gattcgcagc actcgccacg
     5581 ggtcggtgtg cacccactcg ctcgggccgc gcgagtcgag caaccgttgg tccgtcgtcg
     5641 agctctgcac ctgccgacgc cgcatgacga cgggaccacg ccgctgttca cgcggtcgtc
     5701 ctggctggaa gtccgtcatg cgccaaactt aggcgatctc ggcgccgcgc tcaggcggcc
     5761 tccacaacgc gcagccgggg cacaccagcg tcacgagagc caggcggtca tctgcgccag
     5821 gcatgatcgc agctgcgcca ggggtacgaa ctcgtgctgg gtgtgcgcga gctcggggtt
     5881 tccaggcccg aagttgaccg ccggaatgcc catcgcactg aaacgggcca cgtcggtcca
     5941 gccgaacttc ggattggcga caccgccgac cacctcgagg aacgccgcgg cggccggcag
     6001 gtggagacct ggcatggcgc ccggcgccga gtcgacgacc tcgacgtcgt acccgttgaa
     6061 gagctcgcgg aggaacgtct ccgcctcctc cactccacgg tccggcgcga accggtgatt
     6121 gacccgcacc gtgcacaggt cgggtacgac gttgccggcg acgcctccgt gaatgccgac
     6181 ggcgttgaga ccctcgtggt agcgcagccc gtcgatctcg ggacgacgtg gttcgtacgt
     6241 cgccagccgg tcgaggatgt cgcgtgccgc gtggatggcg ttcacgccgt tccaggagcg
     6301 ggcagcgtga gctcggctcc cacgggcggt gacggctacg gtgatggtgc cctgacagcc
     6361 cgcctcgatg acggcgttgg atggctccat gacgacggcg aagtcaccgt gcaggatctc
     6421 ctcccgcacc tcggcgatga gtgtcagtcc gttcagctcg gcctcgactt cctctgcctc
     6481 gtagaagaca taggtcaggt cgcggcttgg tgagtcgagc gtggcggcga gttgcagcgc
     6541 gaccgccaca ccgcccttca tgtcgcacga gcccaggccg tgcagcgcgt cggcttcgat
     6601 ccgcgacggc aggttcgcgt tcaccggcac cgtgtcgacg tgaccggcca cgacgacccg
     6661 ttcgggcagc cccaactgag ttcgagcgac cagcgagttc gagtgccgcc acaactcgag
     6721 ccaaggtaac cccgacaacg cggcctggat cgcgtcagtg atcgactgct cgtgaccact
     6781 cactgactcg atgtcgacga gggctgccgt cagatcgacg atgtcttggc ggggatcgag
     6841 cgcaggcatg cgcgacattg tgacggacga cacgacgcca tgcctgagcg gccgctgctt
     6901 ggccgcgagt ctgtagcgtt gtcgcgtgac tgacacgagg gctgcggcct ggggcttcgg
     6961 cctggcgacg tacgacgcga agggcatcct gctcgacgcg tggttccccg aaccgcacct
     7021 gggtacgccg cctgccgact accacgcgcc gagcacgttg gccgcgctcg agggggacga
     7081 cgccgtacgc gatgtcagcc agtcgctgct gcacgtggtc atcgacgact tgggcgctcc
     7141 gccgcgagac gccgccgagg tgtggctcag gctgcacttg ctcagccacc ggctcgtcgt
     7201 accccacgcc gcgaacctcg acggggtctt cgccctcctc accaacgtcg tctggacgaa
     7261 cctcggcccg tgcgcagtcg aggacttcga ggccactcgc gtccgcgcgc gagagtccgg
     7321 aaaggacatc accgtgctgg gcgtcgacaa gttcccgcgg atgaccgact acgtcgtccc
     7381 caccggcgta cgtatcgccg acgcggaccg ggtccggctc ggcgcgcact tggctgaggg
     7441 caccacggtc atgcacgagg gcttcgtcaa ctacaacgcc ggcacgctcg gcagcgcgat
     7501 gatcgaggga cgtatctcgg ccggtgtgac ggtaggcgag ggcacggaca tcggcggcgg
     7561 cgcgtcgacg atggggacgt tgtcgggtgg cggcatggac atcgtctcga tcggccgacg
     7621 ctgcttgctc ggcgcgaacg ccggcatcgg gatctcgctc ggcgacgact gcgttgtcgc
     7681 ggctggctgc tacgtgaccg ccggcaccaa ggtcacgttg accgacggct ctgtcgtcaa
     7741 ggcgcgcgaa ctgtccggcc agccgggctg gcagttctgg cagaacagcg tcacgggggc
     7801 tgtggaggcg atgcgacggg agggcagcgg ggtcgagctc aacaccgctc tgcacagcaa
     7861 tgcgtagggt cgggcgctac gtcggcggcg tcgtggtcat cggactcctc gtgggcggcg
     7921 gctacgtcgc cgtacagcag ggcctcggac cactgccgga cccggatcgc tgtacggcga
     7981 cggcggacgg ctacaccgtc gagatctcgc cggagcaggc ggagaacgcc tcgatcatcg
     8041 cggcggtcgc agagcgacgc ggacttcctg ctcgagcgat ctcgatcgcg atcgcgaccg
     8101 ctttccagga gtcgaagctt cagaacctcg acagcggtga ccgggactcg ctcgggctgt
     8161 tccagcagcg tccgagccag ggctggggca cgccgaagca gatcatggat ccgttctact
     8221 ccgccaacgc gttctacgac gctctggtca aggtcgacgg cttcgaggac atgccgatca
     8281 cggaagcggc gcagcgagtg cagcggtcgg cgttccccaa cgcgtacgcc gaccacgagc
     8341 gggacggccg gacgatcgcc tcggcgatgg ccggctacag ccaggcggcg ttcacgtgta
     8401 cgacggacac tcccgacctg agcgggcagg acatgacgag ctccgggttg actccccggg
     8461 ccgaggtcgt acgacgtgac gtcgagaagg ctttcgggac catgtcgctc ggtggcttcg
     8521 ccccgggcgg tgtcgacagc ggccacatga acgggtcggc gcactatgac gggcgcgcga
     8581 tcgacgtgtt cttccggccg atctcaccgg agaacacgcg gcgaggctgg gtgttggcgc
     8641 actatctcgt cgcgaacgcc gcccggctgg atgtcgccca cgtgatcttc gacggaaaga
     8701 tctggacggc aggcgacacc tcggatcagg gctggcgcgt gtacacgccg aaggcggccg
     8761 gcgacgtgtc accggcgacg aaggcgatct tgttgcaccg cgaccacgtc cacgtcgacg
     8821 tcttctgacg tgcctcctcg cggctggcgt ggacggtctg cctcccccgg gaggcggcca
     8881 actgccttgg gagggtgagt gaacgcggtc tcgggatgtc ccgacgggct cgctacgtgg
     8941 agagacgccg acaggcggct tcgatccgct cgtcggtggc ggtgagcgcc acgcgtacgt
     9001 gctccgtccc ggacgggccg tagaagtcgc cgggcgcgac gaggatgccg cggtcggcca
     9061 gccacgccac ggtttccaga cacggttggc cacgcgtggc ccacaggtac aacgagccct
     9121 ccgaatggtc gatccggaaa cctgccgacg tgagggcgcg acgcagcaga ccccgccgct
     9181 cctgataccg ctgccgctgc tcctcgatgt gagcgtcgtc gtccagcgcg gccgccatcg
     9241 ccgcctggac ggggcctgcg accatcagtc cactgtgccg gcgtacctcc cgaaccgcac
     9301 ggacgatctc cgggtctcct gcgacgaacc cggcgcgata tcccgccatg ttcgaccgct
     9361 tcgacagtga atggatcgca agcaccgact ccagcgaccc gccgttgacg gccgggtcca
     9421 gcaccgagac cggctccgtc tcccatgcgc actcgaagta gcactcgtcc accgcgacga
     9481 cagcaccgcg ttcgcgtgcc caggcaacca ccttccgcag gtgctcgatg ggcaggatcc
     9541 ggccggtcgg gttcgccggt gagttgagcc agaccaacgg cacatcacgg gggccgaccg
     9601 acaccgtggc gtcactggcg acgtagtcgg cgccggccag gagagcgccg acggcgtacg
     9661 tcggataggc gacctgcggc acgagtacgg tctggccggg gtgcacgccc aactgggacg
     9721 gcaacgccgc gaccagctcc ttcgagccga tggtcgggag gacgcagtcg tcgtcgagtt
     9781 cgaccgaacc gcagcgccgg gtcaggtacc cgatgatcgc ggcgcgcaac gtgggtgtcc
     9841 cccacaccac gggataaccc ggcgagtcgg cagctgctcg cagcgcctcc cggacggatc
     9901 gcggagtcgg atcgacaggc gtcccgacgg agaggtccac cagtccgtcc gggtgggcga
     9961 ccgcctgctc cttgaagccg agcagacggt cccacggaaa gtcaggcagc acgacgggag
    10021 cccgtccaga ccgtgtcgcc gcggcctcag cagacggact catcggccgt caggagatgt
    10081 caacgcggct cacgaaactg tggacggctc actcgccttc ttggttctgt ggagggagtg
    10141 ccgcgatgaa cgggtggtcc ttgtcgatca cgcccagctt cgccgcgcca cccggtgagc
    10201 cgaggtcatc gaagaactcg acgtttgcgg cgtagtagtc cttccactgc tccggcgtgt
    10261 cgtcttcgta gaagatcgcc tcgaccgggc agaccggttc gcacgcaccg cagtcgacgc
    10321 actcatccgg gtggatgtag agcatccgct ggccctcgta gatgcaatcc accgggcact
    10381 cgtcgacgca ggccaggtcc ttcaggtcca cgcatggctg cgcgatgacg tacgtcacct
    10441 cgtcctcctc ggtctcagcc caccccggtc catgccggtg gcgctccaac acaaactagt
    10501 atctacgccc tcgtcgtggt cgccgaactc aggtccaccc gttgtcgagt gtgtcagcca
    10561 caggagtctc atgccgttgc agcgtcgtca cgtcggccag cgtgtggtcg tacgccggct
    10621 gctgccgggc cagcagggtc ccagcggcgg acccgccatg accgacgtga ccggcatcct
    10681 cgagacgtgg gatgagggat cgttcgctgt ccggcgagag gacggtgatc tcgtcacgat
    10741 cgagcgcgac ctgttcgtca ccggaaagcc gatcccaccc cgcgcgtcga cccggctgcg
    10801 ggtgaacccg gagcggctcg agcgcatctg cgagggcggc tggcagccga tcgaggctca
    10861 acctctcggc gaatggacgc tgcgagccgc cggcggcttc accggacgag cgaactcggc
    10921 ccgcgtcggc ggcgatcctg gggtcgccac cgacgccgcc ctcgagacgg tcgccgactt
    10981 ctacaccgcc cggggactgc ccgtgctcgc gcaagtcgtg gtcgggtcgg actggcagac
    11041 cgccttcgtc gaacgtggct gggtgacggc gcggcatgcc ggacaggatc ccgtcgtcca
    11101 ggttgcctct gtggcgagga cacatcggcg ggccggtgag cgcgcatccc gaaacaccga
    11161 ctcgctgccg gcgcgtctcg acagcacacc gagtcccgac tggctcgccc tgtacgggcg
    11221 caccgcgggc atcgacgcga ccgtggtccg gcgggtgatg gaatccggcg acgaggtggc
    11281 gttcgcccgc atcggcgacc cggtcctggc gatcgggcgc ggtgtggtca ccggccactg
    11341 gcttggcctg caggccgtcg aggtggtgtc gagccggcga cgccaggggt tggcgaccgc
    11401 ggtggtcgac acactgctgg cctggggcgc ctcgcacgga gccctgtcgg cgtacctcca
    11461 ggtcctgccg gacaacgatg cggcactggc gctctacgcg gggtacggct tcaccacgca
    11521 ccacgcctac cgctacctca cgccgtccac gtagcttccc gcgctaccgc atcggactgt
    11581 tcggccgacg ttcccgtccc gtctggctgg tgtgtgtgag ggcggtgttc gggcatccgt
    11641 cgatcagctc ttggcggtcc gggatgctca catccgagac cgcctgtgcc catctcaggc
    11701 cccctgtgcc tatccgaggt ttgcgatgtt tacacgcttt ttgggctgta aacatcgcaa
    11761 acctccaact cagaccgacc cttgacggga ggaaggcaag gtcgcaggcg cggccgcgca
    11821 gagacgtgag gagagtctgc attggctgaa gccacccaga gcgccgtcag gggcgggagc
    11881 gggcggtgca gcgaacttgg tcgagcgggg catagctggt gtgagtgacc tcgctgcgac
    11941 gatggccgtc tcggcgcagt agccgagtca cgtcgacgtc gaacccggct gcacccgcac
    12001 ggggcacgca ccccctctgg gagttcgtca cgacgtccgg cggtacgacg gagtagcggc
    12061 cggacgacct ggccgacact cgccagtagc gggtgctcca gatctccaca cgcgccaccc
    12121 ggaccggtga gcctcggcca ccagggctgt cgacgtacgc gcggacatag acgccgtacg
    12181 gcgtggagtt gcgcagcacc aggtcggtga ccggcggctc gaccgcggcg tccatacccg
    12241 gcgggaagtc tgcggtgtag atccgggccg acgtgcgctc ggggatgtcg agtccggcga
    12301 agaaggcggc gttgtaggtc agcgtggcca cggtcgaggc ggcgggtgta cgtccgtccc
    12361 ggacggccgc caggaagctg aacgtcgatc ctggcctgat gagctcgccg ttcagccgtc
    12421 ggagcgcgtc ggtcggatcc gtgccgccga cggccggacg aatccgaagc tgtgcagccg
    12481 ccacgatccc ggtcacccgc aacatccgca catcgtgcgt cgacaccctc ggagtgtcgg
    12541 cctggacgtg ggcgcgcgcg acgcgctctc cgctcggtcg gccggccgcc gccaggacag
    12601 ccttggccag atcgtcgggt gtgacggtga ccccgctggt cgccggtacg acgaccggac
    12661 ggtcaccgcg gaaacggatt cgggcgttca ccggatggtg gggcagtcgc cgcagggcgg
    12721 gcttgacccg ctcgtacaac cggtcctcgt cgacgtccag gcgcagtcca tcagatctcg
    12781 acaccgcgcg cagtgccgtc gcgaacgtcg cgggctgcag cgtgagcgcg tcgtcggcca
    12841 ccttgatccg gaccggcccc gacaccgcgc ggcgaccgac cgtctcggcg aaccgggtcg
    12901 cctcgatcgc ggtcacgtcg ggctggaccg ctttgatcgg cagtgtcgtc tggtcgtcgc
    12961 cggcgacaag cgccgcgatg agccgatccc cgctcagctg gtagtccagt gaacgaccgg
    13021 catgcccggg agccacgacc ggcctgcccg accggaacga gacgaccgac tcgaccggct
    13081 tcagctcgat cttctccgcc acctgccgca gtcgcgccgc gagctcgtgg ttgtcgacgt
    13141 cgacaaccgg cgccaggtcg tcaccgccgg tcagcacgtg cagcatgtgt cgcggatccc
    13201 acgggctgcc ccccagcgcc ttgtcgatgg tggcgtcgat gtcgaaggtc agaccggcgc
    13261 gctgcggttg gaaggtgaag tcctcgccgg cggcggtggc cttgatcggg cgttgcacgc
    13321 gcggagccag cctgtcctgc agaaccgtcc gagcctcgtc aggggtcagt ccgccgacat
    13381 cgacggcggc gatcgtggtt ccggcgggca gcttgttccc tgccacggcg tatcccacgg
    13441 cgtacagacc accgaagacg accaacagac ctacgacgaa gacgacaccg aatcgaaggt
    13501 cgtgcgtctt cggcaagttg tccagataga ccttccggcg aggccacaat ctcacggcgg
    13561 ggcccccgga ctcatagcag acctcgcggg gtcatcgcgg tcctcccgcg gcagccatgc
    13621 cgatcaccgg gacggacgag agaacgaggt acccgatgcc gagcgcgtcc tcggccagca
    13681 ggtagtcgcc ctcgggacgg cctcccacga ccagggccgt gccgatgatc cagccggcgg
    13741 cggcgacgaa gccgaggacg cgcgtgtacg tccgggccag atagacgatg gacaccgaag
    13801 cgaccacgcc gaggaccagg ccccacggta cgacgacggt ccccggtcgc caggccattc
    13861 gcgacaccag agccgacaac accgacacag cgaacgacac caccgccacg acgagagcga
    13921 gaaccagtat cgccggtctg ctcgcaagcc actccggacg accgacgtcg atctgcggtg
    13981 ctcgcgttga acgcttcgtc gcctgggagc cgcccccgcg gtcgaggttg ctcgtcatcg
    14041 aaggtcaggc cactcccgcg aagaggctgg tctcgtgacc gtcgacccca cgctcacctg
    14101 ccacgccctt cacgagccgg taggactcga ccccccagac ctggtttccg atgttgttcg
    14161 acagggcgaa gaacggaccg tccaccgtga tctgggtggc gtgcgcgcgc atcgcgtcca
    14221 gcttcttgtc gacgtggtcc atcgcatcga tggtggcggt gatcagctcg tctgcagtga
    14281 cgaacggcgg cagctcaccc tcggggtcca tcccctcgaa cgtcgtcagg tcgccgctgg
    14341 cccgcagccg gcgcagccct tcacgcatcc gttgctccga catggcgttc cagtagacct
    14401 tcgcgacatc ccagggttcg ccgagctcgc gtcggaagct cggtgcagcc gccagcacac
    14461 tgccgtacat cgcgacccgg tgtgcctgga tgtggtcggg gtgaccgtag gcgcccttct
    14521 cgtcgtacgt gacgaggacc tgcggacgca cctcccggat gacttcgacc agcaagctcg
    14581 ccgcctcgag caggtcggcc gcccagaacg cgtcgtcgcg gatcttgggt ggtgggatgg
    14641 cgtgcccgtt ctcgtcgacc gccatcccgg tgtcccggaa ccggccggca ccgccgagga
    14701 accggtggtc gatgacgccg aggatccgca tcgcatcggc gagctcaccg atccggtgct
    14761 caccgaggcc gtcctcgtga tgtgaggcga ggtgctccag ctcggggacg agcacctcac
    14821 cctcctcgcc gagcgtgcac gtgacgagcg tgacgtgggc accttcactg gcgtacttgg
    14881 ccatggtggc gccgttggcg atcgtctcgt cgtcggggtg cgcgtggacc aggagcatgc
    14941 gccgatcagg cagcggctgc gctgtcatgc gcccaagcgt acggcccggc acacgccgga
    15001 cccgttcgcc actcgcccgg catcggtcgg tccggtggag tgccccgcga agactaagaa
    15061 cgctggagct cgcggcggat ggcggggatg cgctgggcga gattggcgag ggatcgtgtc
    15121 gtccgctcgg gctcgccgac ggtgcgcagg ttgagcatgc ccgggtcgcc ggccatctgc
    15181 ccggcgcgga tcacgtcgag cgaacgtcgg atcctgggct ggaccgcgtc gacgatgtcg
    15241 accgcggtga tcgcggcgcc gtacgccgac gccatcacct cgacacgctc ggcagcgagt
    15301 gccggagcga ggtcacgcca gagcgggacc cagttccagg cggcgaacgc caggtcctgc
    15361 agcgcagtac ccggaccgga catgtcccag tcgaagactc cggcgagaca ttcaccgtcg
    15421 ggagaccgcg tcagcgcgac gttgtacggg gcgaagtcgt gatgacagac gatctcgtcc
    15481 gtcgccaggc cacggtggac cgtacgccag gtgagcccgg cagccggccg gaactcgcgg
    15541 accgcctcgt ggaaccgacg tagccagcgc atcgcgtcgg ccagcgtcga ctcgtcgagc
    15601 acttcgacat cgacgtccgg cacccggccg ggaaggaacg tgagcacctc ccgaccacgc
    15661 ccgtcgtacc cgagcacctg cggagcctgt acgaggccgg cggcacgcag atggtcgagc
    15721 agggcgtgca ccgcctcggt ccacgggccg gtcgcgcggc gtaccgtccg gccgactcgc
    15781 accgcacctg cgacgttgcc cccgggcagc cgctcgccgt ccagcaacag ctcgcgcagc
    15841 gtcggcagga acggtcgatc cgcaggcagc cactcgacat cacccagctc ctccggcccg
    15901 agccagcgga ccgcgtcgtg ttcgtggggc accggttcgc ccgacacgag acggaccagc
    15961 agcacggtga ggacgtagcc cggcttcacg tccacgcgac cctcgatcgc accgacgacg
    16021 gcgaccgagc acccgagctc ctcgcgcagc tcgcgcacga ccgccgcttc ccgggtctcg
    16081 tcgggacgag ccttgccgcc cggcagttcc cagcctccgg ccacgtcggg cgggtggaca
    16141 cgacgagccg agagcacccg gccatggtgc agcaacgccc ctgccacgac ctcgatgtcg
    16201 ggcggtgcag ccatcgcatc cacctcgggt gactcgggca cacggcagcc ttgcacacag
    16261 gcgatgtcgc ccatccgacg gtgacgactg cctaccgagg gatgcggcaa tgcggcagct
    16321 tgatgtcgag ggaatcagcg gccttcggtg tctctccacc gtgagctcga tcagcgggga
    16381 gtgtctactt cctcacgtgc ccgccccttt cgcaatgctg caattcgcgg gagacttcaa
    16441 gccatccgcg caccggtcga cgtaccgagc cgacgagcca gccgtcgcac tgcgtcgcgg
    16501 acttacgagc agtcgacgac ggaggaccag gcgtggcgtc gcacgagtgc gggcacagac
    16561 ccggtcaggc gcgagacgac gcgatgtggt ctgatgccga agacgcgggc tgcgccgacc
    16621 cgtaccgcgt gtcgtcctga cgagagaagg ttgccttcgc atgtcaacgg gagtcatggg
    16681 acctgggcgg gtccgggtgc agacgaggac acttcgccag gaccggtggt ggatcgcccc
    16741 gttgacgacc ttcatcgtct tcggggcgtt cgtggtctac gcgacctggc gggccttcga
    16801 aggcgccaac tactactcgg cgccgtacct gtcgcccttc tactccccgt gcctgaccaa
    16861 cgactgcgtc gagggatcgt cggacttcgg ccatccgttc tcatggtggc aactctcccc
    16921 tgcgctgctc atcttgatct tcccgctcgg cttccgactg acctgttact actaccgcaa
    16981 ggcctactac cgggcgttct ggctgtcacc accggcgtgc gcggtggcgg aaccgcacgg
    17041 caactacacc ggcgagacac gcttcccgct gatcctgcag aacatccacc gctacttctg
    17101 gtatgccgca gtcctcgtct cactggtcct gacgtacgac gtcgtactgg cgttccagga
    17161 cgaggacggc gactggggcc acgtgggact cggcacgttg atcatgctgg tcaacatcgt
    17221 gctgatctgg ctctacacgg tgtcctgcca ctcctgccgg cacgtggtcg gcggacgact
    17281 acgccacttc tccaaacatc cggtccgcta caaggcatgg acgttggtct cccgactcaa
    17341 caagagccac gcggtgtacg cctgggtatc cctgttctcc gttgccctga cggactacta
    17401 catctacctg ctcgccaccg gcgctttcga cgacccgagg ttcttctgat gtccaccgag
    17461 acgacccacg gccccgacga gaaccggcac ccgatgaccg gcaacctgat gtcgagcgag
    17521 gagacctcgg tcgacggcca cggcttcgaa gagcacgagt acgacgtcat cgtcatcggt
    17581 gccggcggcg ccgggctgcg tgcggcgatc gctgcgttcg acgcgggagc tcggaccgcc
    17641 atcatctgca agtccctgct cggcaaggcg cacacggtga tggccgaagg cgggatcgct
    17701 gcggccatgg gcaacctgtg gccggaggac aactggcagg tgcacttccg cgacacgatg
    17761 cgcggcggca agatgctcaa ccactggcgg atggcgcagc tgcacgcgca ggaggccccg
    17821 gagcgggtgc tcgagctcga ggagtggggg gcgctgttcg accgcaccgc cgacgggctg
    17881 atctcccaac gcgacttcgg cgggcaccgg tacgcacggc tcgcacacgt cggcgaccgg
    17941 accggcctgg agatgatccg taccctccaa cagcgcgctg tcgccctcgg catcgacgtg
    18001 ttcatggagt gcacgatcac cgacatcttc aaggaccctt cgacaggctc aggacaagca
    18061 cgcggaggtg gtggccgcgt ctcgggcgcc ttcggctact ggcgcgagtc cggccggttc
    18121 atcgtgttca aggcgccgag catcgtgttg gccaccggcg gcatcggcaa gtcgtacgag
    18181 gtcaccagca actcctggga gtacaccggc gacggccacg cgctggcgct gcggaccgga
    18241 gcttcactca tcaacatgga gttcgtgcag ttccacccca ccgggatggt ctggccacct
    18301 tcggtgcgtg gcctgctggt caccgagtcg gtgcgtggcg acggcggcgt actgcgcaac
    18361 tccgagggca agcggttcat gttcgactac atcccggagt tcttcaaagc cgagacggcc
    18421 gacaccgagg aggagggcga ccgctggtac agcgacaaga ccaacaaccg gcgccctccg
    18481 gaactgcttc ctcgagacga ggtggcgcgg gccatcaact ccgagatcaa ggcaggtcgt
    18541 ggatcgccgc acggtggagt gtttctcgac atcgcgtccc ggcgcagtcc ggagttcatc
    18601 aagcgccggc tgccgtcgat gtaccaccag ttcaaggagc tggccgacgt cgacatcacc
    18661 gcggaaccga tggagatcgg accgacctgc cactacgtca tgggaggcgt cgaggtcgac
    18721 ccggacaccg gcgagagcct cgtgcagggg ttgtacgccg cgggtgaggt ggccggtggc
    18781 atgcacggct cgaaccggct cggcggcaac tcgctgtcgg acctgctcgt gttcggtcgc
    18841 cgggccgggc tcaatgcggc gtacgccgcc caacgcacgg cggcgaaccg acccacggtg
    18901 gctgaagagg acgtcaccgc ggccgccgcc cgcgcggtgg caccgttcga ggagcagggc
    18961 gagaacccct acaccatcca gcaggacctg caggacacga tgcagcgact ggtcggcatc
    19021 attcgcagcg ccgaagagct caccgagtcg ctcggcgaga tcgacaagct gcgtgcacgt
    19081 gccgcccgtc tgtcggtcgt cggcaaccgc cagtacaacc ctggctggca cctggcgatc
    19141 gacctccaga acatgctgct ggtctcggag tgcatcgcac gggcggcgct cgagcgcaag
    19201 gagtcgcgcg gtggacacac ccgtgacgac tacccgctcc ccgaccccgg atggggcgcg
    19261 cagaacctcg tcctcacgtt gaacggcgag ggccggatcg acctcgagca ccaaccgctg
    19321 cccgtcatgc ccgacgagct ccagtccttc ttcgacaagt gagagtgacc cgctgatggg
    19381 atatgacctg aagctgcgcg tgtggcgcgg cgacaagacc ggtggcgagc tcggcgacta
    19441 caccgtgccc gtcgaggccg gggaagtcgt cctcgacgcg atccaccgct tgcaggcgac
    19501 ccaggccggc gacctggcgg tgcgctggaa ctgcaaggcc gggaagtgcg gctcgtgcag
    19561 cgcagaggtg aacggcacac cgcggctgat gtgcatgaca cggttgtcgg acttcgagcc
    19621 gtcggagacg atcacggtga cgccgatgcg agcgttcccg gtgatccgcg acctcgtgac
    19681 cgacgtgtcg ttcaactacg agaaggccaa acaggtgccg gcgttcgccc cgacaccgcg
    19741 cgatcccgac ggcaaacgcc ggatgcagca gtccgacgtc gaacgcggtc aggagttccg
    19801 caagtgcatc gagtgcttcc tctgccagaa cacctgccac gtcgtccgcg accacgagaa
    19861 caacaaggaa gcgttcgccg gcccgcggtt cttcctccgc ttcgccgagc tcgacatgca
    19921 tccgctcgac acgaacgacc ggcgcaggct cgcccaggac gctgccggaa tcgggatgtg
    19981 caacatcacg aagtgctgca cggaggtctg ccctgagggg atcaagatca ccgacaacgc
    20041 gatcatcccg atgaaggaac gcgtggtcga ccgcaagtac gacccggtcg tctggctcgg
    20101 ctcgaagatc ttcaagcgct ccgatcgcgc gtcccgagcc gaggtctaga ccctgcagta
    20161 cgccgttcga cgtctcggtc cgagggtgcg acgtcatctc gccataccgt ggcttctcag
    20221 cggcgcctca gccgaggttc cggacctggt cctcttgtga acggcaggtg ttctgggttt
    20281 gccctgctcc tcagctgagc aagggcgcac gtccgaccta ggctcggctc gtggtcgact
    20341 ggtgggacgt ctacgcacga ctgggcacct ggttgcggtt cggtgtctcc ggcgacggaa
    20401 ttcgcctgca ccacggacgc gacctgggtg atcgcgtcga tgcgctggta ggccggctgg
    20461 gaggccgctg cgggctggac ggcgtactcg acgacctcga ccgtgccgcc accgtcgtcg
    20521 taccaccgac caacgccgcc gactacgcgt tccggtggaa tgacgaggac ttcgacgatc
    20581 cggtctggtg gccgcagggc atcacgacct cggccgacgc aggccaccgg cccgacggat
    20641 cgacggacac aggacgcaag gtcgtggtcg tcgcctggta cgccaagcgc gtcaacggct
    20701 tgacccaggg agtgcggctg tccttcgtcg acgtcaccga ccacgcgcac cctcggtacc
    20761 ggcacgtcct gctcgtcgaa cccacccgac gacggtggag ccggaaggtc ggcatgcggc
    20821 cggtgccggt gcatgccggg gggatcgtct ggtacggctc ggcgattctc gtcgccgaca
    20881 ccttgggagg catccggacg ttcgcgctcg acgacgtctg ccacccgccg ggagcgtggc
    20941 gcggccacga ctacgtcctg ccacaacggg cgtcgtacaa agccatgaac gaccgtggcg
    21001 tgcggccttt ccgcttctcg ttcgtctcgc tcgaccagac cggccaccag catcagctga
    21061 tcgccggcga gtacggccgg tcgggctccc agcggctcgt ccggttcgcc ctcgaggccg
    21121 accgcgccgc gccggtggtg gagcgcggcg tcgcccgccc gatcgagctc gtcacggaca
    21181 cgttcacgca catgcaaggg gcaacgatgg tcgacggcgt gtggtacatc tccagcagcc
    21241 gcggcccgag gccgggatca ttgtgggtgc gccggcccgg tgacccggcc acggagcacc
    21301 gtgaagcact cgcgacctca ccggaagacc tctcgtactg gccgcacacc gacacgctgt
    21361 ggaactgcac ggagaaccct ggcatgcgct acgtgtacgc gatgccgcgt tcgcagttcg
    21421 gcaccgaaaa gcgctgattg ccaggatccg ctgatcgcca aaatccgctg atcgagcctg
    21481 tcgagatcga ggatcccagg ctcgaccgcc ggatgccggc tgatcgagcc tgtcgtgtgc
    21541 cggggtctcg acaagctcga ccgccggatg tccgctaacg agcctgtcgt gtgccggggt
    21601 ctcgacaagc tcgaccgccg gatgtccgtt gatcgagcct gtcgagatca acgcacggca
    21661 ggtggcttgt tgagccggac gcggcgactc cgtccctcgc gtccgtccat caggccgggg
    21721 cagtcaccgg cggtggcgac ggcgtcgacg ccgcaggttc tgccgggttc accatcgacg
    21781 ggtcgtcttc cgggccgagc ttgcacgacg caacggacgt caacaccgaa agcagggcaa
    21841 ccatgccggc cgtggacagc gccgtcatcc accgagcggt cagcagaccg agcccgctgg
    21901 cggcgagcac cgcggccaac gactgtgcga acgacttgat cgcgcgttcg gccgtcttct
    21961 tccagaacag ggcgctgaac atggtcgact cccctcaagg cgacccggtg atggttgcgg
    22021 gtgaggaggg gggctgccgc tcgtcagata cacccgcgct gttcggcgta cggtgaggtc
    22081 atggctgacc tgctgacacg ggaacagatc gacgacgcac tgggatcgac gctcaccgat
    22141 tggacgctcg agggggacga caccatccgg cgcgacgtca cggctgacac gttcgcgggc
    22201 ggtatcgcgt tggtcgacaa ggtcgcggtc gtcgccgacg agatgaacca ccaccccgac
    22261 atcgacatcc ggtggacgac gatcaccttc cgcctgaaga cccactccgc gggcggcctc
    22321 accgacaacg acctgcagct ggccgccgag atcaaccgac ttgtcagaag tacatagagc
    22381 tatggctcga cctgcttctg acaactcgat aaagtgaggg tccgatgacc ctcaaccgca
    22441 tgacgatcct gatttctgcg atcttccttg ctctggccgc cgcgatcggt gtggctgccg
    22501 ccgccggtgg ccagtccggt ggcccgtcac acgacaagct gatgccgatc ggcacgatga
    22561 cgctgctgcc gtgggacgcg cacgagctcg ccatgtacaa ggtgacctac cacccgcatc
    22621 agatgtgcga cgacgtacgc cacccggcgc acgccaagag cgcgatgtag gtcgtcggct
    22681 cgtctgctgc ggagctcccc cgcagcagcc ttgacctacg cacgcggcca gccccgacgg
    22741 atacggtgtc gcggtggacg ctgacgtcat cgtcgtaggc gcgggactcg ccggtctggt
    22801 tgccaccgcc gagctcgccg acgtgggtcg gtcggtcatc ctcctcgacc aggaaccgga
    22861 gacgaacctc ggtggtcagg cgttctggtc gttcggcgga ctgttcgtcg tcgactcccc
    22921 cgagcagcgc cggatgggca tcaaggactc gctcgacctg gcctggcagg actggtccgg
    22981 cactgcgggg ttcgaccggg acgaggacca ctggccgcag cggtgggcgc aggcgtacgt
    23041 cgacttcgcc gccggtgaga agcgccgctg gctgcacgac caggggatgc ggttcttccc
    23101 cgtcgtcggc tgggccgagc gaggtggcta cctcgcgacc ggccacggca actcggtgcc
    23161 gcgcttccac gtcacctggg gcaccggccc gggcgtcgtc gagccgttcg agcgccgggt
    23221 gcgtgcggcc gtcagccgcg gacgggtcag gctccggttc cgccaccggg tggatgagct
    23281 ggtcgtggcc gcagggacgc cgaccggcgt acgcggtgcc gttctggagc cgagtgctgc
    23341 cgcccgtggc gctacgagct cacgcatcgt cgtcggcgag ttcgagtacg gcgcccaggc
    23401 cgtcgtggtc acgtccggcg gtatcggtgg tgaccacgat ctcgtacgcc gcaactggcc
    23461 ggcccggctc ggtccaccgc cggccgagat ggtgaccggc gtccccgcgt ccgtcgacgg
    23521 ccggctgctc gacatcacgg cgaaggcagg cggtcacgtc atcaacccgg accggatgtg
    23581 gcactacgtg gagggcatct cgaactggga cccgatctgg ccgggccacg gcatccggat
    23641 cctccccggc ccgtcgtcgc tctggttcga tgcgctcgga cgacgcctac ccgccccgct
    23701 gttcccggga ttcgacaccc tcggcacgct ggcgtacctg cgatccaccg gacacgacca
    23761 cagctggttc gtgctcacgc agaagatcat cgagaaggag ttcgcgctgt cgggctcgga
    23821 gcagaacccc gacctcaccg gcaagagcgt ccggcaggtc ctcggccggg tccgctcggg
    23881 agcaccggga ccggtcgagg cgttcaagcg gcacggcagg gacttcatcg tggccgactc
    23941 gttcgccgag atggtcgagg ggatgaacgc gttgaccgac gagccgttgc tggaccatga
    24001 cgacctgctc cggcaggtcg aggcgcgtga tcgggagatc gacaacccgt tcacgaagga
    24061 cctccaggtc atggcggtcc gtggcgcccg gggctaccgc ggggaccgcc tcgtccgggt
    24121 ggccgcaccg caccggatcc tcgacccgaa ggcgggtccg ctcatcgccg tcaagctgca
    24181 cgtgctcacc cgcaagacgc tcggcggcct cgagaccgac ctcgacggcc gggtgctgcg
    24241 tgccggcggc gagccgctgc ccggtgtgta cgccgccggc gaggtcgcgg gtttcggcgg
    24301 cggtgggatg catggttatc gggccctgga ggggacgttc ctcggtggct gtctcttctc
    24361 cggtcgccag gccggccggg cggtggccgc tgcgaccgcg tgaatcgacg tacgccgcgc
    24421 ggtctcatcc gtcgctgacg ttgaaccgtc ggtgccgcca gtccgtggtt cctttcggca
    24481 ggtacaggca cgccaacgaa cggatccgaa ccaggagtgg cgatgaacag acaccatgca
    24541 cgcagatccc agacactcac ggccggcggg gccgccgcag caggcatcct cgccctggcc
    24601 ctcacggtcc cgacgggagc ggtgagcgca gcctcgccga cctcacggca cga
//
LOCUS       DATBFZ010000245         9644 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_1146075, whole genome shotgun sequence.
ACCESSION   DATBFZ010000245 DATBFZ010000000
VERSION     DATBFZ010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 9644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9644
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1146075"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2127
                     /locus_tag="VH419_07585"
     CDS             <1..2127
                     /locus_tag="VH419_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011753726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEY7043512.1"
                     /translation="PTLSYDTQAVLATDKVRFQGQEVAAVIADDPYIAKDACEAIVVE
                     YDPLPVIVNPYQATAQGAALIRDDKDNQHDNVIYDWEVGDRDGTDRAFADADVVSELE
                     LHYPRSHPSPLECCGSIADFNRSTSKLTVYMTTQAPHIIRAAVALVAELPEHMIRIIS
                     PDLGGGFGNKVPVYPGYVVSILASILTERPVKWIEDRTGNLISTGFGRDVYLKGELAL
                     RRDGRILAVRMHTDADHGAFFSDAQPSKFKIGLMHSAFACYDIPYAHLTARGMYTNKA
                     PGGVAYRCSFRITEAMYFQERMVQAAADDLGMDQAAIRRVNLVRDDDFPHRTPFGFLT
                     DSGQYAKCLDVGLEAIGYQDFLREKEEAKQHGRLLGIGISTMTEPLGAGNSREYDILG
                     IKMFDSAELRVHMTGKAILRTGAKTQGQGHETTWGQIVAHELGIPADDVVVEEGDTDT
                     APFGMGTYASRSTPVAGAAVAMASRKIRAKARRLAAHLLEVSEEDIDWELGRFYVRSA
                     PDRGVTIQECALAAYSNMPDGMEPGLEANAYYDPPNLTWPFACYIATVEVDPDTGVWD
                     VLRTVAVDDCGVRINPMIVEGQIMGGLTEAYAMSSMQYITFDSEGNCVGSNFMDYLLP
                     TAWETPGYELHEVVTPCPHHPIGAKGVGECATVGGPAAFVGAVLDAIKDTGVRNIDMP
                     LLPDRVYEAMTRRQDVSGAPPLRTDT"
     gene            2124..3095
                     /locus_tag="VH419_07590"
     CDS             2124..3095
                     /locus_tag="VH419_07590"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HEY7043513.1"
                     /translation="MSNRALDGWEVIARADELVRGGEEFALATVVWRQGPSSGQQGSR
                     AIVNATGEIQGWIGGACAEPVVIREAQRVIRDREPKLLLLGNPGQFGGAVPDGITVIP
                     IACQSEGALEVYLEPVVPTTHLVVVGRSPMAHTLADFARGLGWRCDLREVADFSAADV
                     GATSVVVIATQGHGDEEALEEAVSASPAFVGLVASRRRGETVLGYLADKGLAREQLDR
                     VKVPVGLDLGRTSHREIAVSILAELVQLRASGALREDGAEDQHGVRQESASTALAVDP
                     VCGMTVAADPSSRPVEHAGTTYYFCSAGCRAAFEKDPQTYITGVSAG"
     gene            3105..3689
                     /locus_tag="VH419_07595"
     CDS             3105..3689
                     /locus_tag="VH419_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011753728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY7043514.1"
                     /translation="MLLTNAFEVDQPVDKVWEFFNDIPQVAACLPGAELTEDLGDGTY
                     AGRVAVRMGPVRLSFNGTADIVERDEAAKRIVVDAAGADEKGRGQASVLVTAALAAKG
                     RGTKVDLQQDLQLSGAAAQYGRGMISDVTTVLMGQFANNMQHRIAAVDAGETPQQASA
                     PAAASGFSIGWQAMRLALARVFRRFFLPYQPRQS"
     gene            3701..3964
                     /locus_tag="VH419_07600"
     CDS             3701..3964
                     /locus_tag="VH419_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011753729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043515.1"
                     /translation="MSGMVLWWVGNVLLLVVVVPILVALLNRVLAALERIRGATDDIL
                     AGGVELAGQLEGVPEGLATTDRTVHEIAVGATRYAGSVAKLLG"
     gene            3978..4205
                     /locus_tag="VH419_07605"
     CDS             3978..4205
                     /locus_tag="VH419_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043516.1"
                     /translation="MPVAAWVTIVISVLIIAAAALGLIRVIFHLRATNRALEGVVGGV
                     RVVAHQTRTVPEVIPSVNDNLKPVRDFCETV"
     gene            4225..4500
                     /locus_tag="VH419_07610"
     CDS             4225..4500
                     /locus_tag="VH419_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011753730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043517.1"
                     /translation="MTSHARSVAISDTTGWVIGYTAAIVVVLVVVALVVPILLLAHSI
                     GSKAKLIDDSLQDSVRHTSALSGLNTTIDHVTVIVAGLNRGRTRLGG"
     gene            4512..4802
                     /locus_tag="VH419_07615"
     CDS             4512..4802
                     /locus_tag="VH419_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011753731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043518.1"
                     /translation="MTVVALTSTNENLWWGAVVGGFVVVLAVAALLTILVLEVRTIDR
                     RVVQVRDTLQAAADNTADTALIATTADGVEAVLAEGLQHHLFLGRVLGKVRM"
     gene            4799..5167
                     /locus_tag="VH419_07620"
     CDS             4799..5167
                     /locus_tag="VH419_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011753732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043519.1"
                     /translation="MTALVVLSVIDIALLIAGLAFYLYVVGSQLTRIATNLEECQALV
                     AQIVRNAEPIIPGVGHINRTGGVVAGALPLLYDMAEAIVTGVAPAPEQPAERPPAAPA
                     SGRRRSRLHDSVGYAPTRSS"
     gene            complement(5162..5611)
                     /locus_tag="VH419_07625"
     CDS             complement(5162..5611)
                     /locus_tag="VH419_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011753733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043520.1"
                     /translation="MIRLGSLAGYPFEGPRLLAGWTPPQAAAVYAVVYRPDPDGKPDH
                     YAVIYVGHSEDLSSERFPFSHPGAPCWVRRAGSRWQVYICTYEVPGGLRSHREQITQE
                     LAAIYHPRCNEQQFERSWKEEWIGAYVAPTTGPLTTGREPGQPTGGS"
     gene            complement(5608..5916)
                     /locus_tag="VH419_07630"
     CDS             complement(5608..5916)
                     /locus_tag="VH419_07630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043521.1"
                     /translation="MRWSVTIQAEGDRVLAREEVVELADAVAASGGIASGIGSSRYGA
                     QLIVEADDLQAALEQGKAEFVRAAERAGLPAWPIASAEAVSESPLDLDGFDRRGGSVP
                     "
     gene            complement(5927..7057)
                     /locus_tag="VH419_07635"
     CDS             complement(5927..7057)
                     /locus_tag="VH419_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011753734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY7043522.1"
                     /translation="MPDDSHPFVETLVAFGGELREAGLSVGSGDVLTYSASLAALDPS
                     DLLDVYWAGRAALVARRDEIDVYDRVFRRFFLGEDAPTESKPYSPEARSAADAVLQVP
                     ETEPGGVSREDREARLGLVASDVATLRSKSFAACTTEELEALHRIMRAMRLSPPRRRT
                     RRTAPAGSGVRPDMRRTIRETMRMHGEPSSLYWRRRRLRPRPLILILDVSGSMADYSR
                     NLLQFAYSTRRVTARVEVFCFGTRLTRITRELEHRKPDEALDRAAKAVVDWDGGTRIG
                     ASLDAFVRDWGRRGVCRGGIVVICSDGLDRGDPGVLAAAMERLSRLSHRVVWMNPHKG
                     DDIDFQPSTLGMMVAAPHIDVLYSGHDLRSLAEFAARLPELS"
     gene            complement(7050..7925)
                     /locus_tag="VH419_07640"
     CDS             complement(7050..7925)
                     /locus_tag="VH419_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020387580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEY7043523.1"
                     /translation="MDRAQTVGDLTQTLRGGGYLADRGLSTALFVALSLQRPLLLEGE
                     VGVGKTEVAKVLAATTGRRLIRLQCYEGIDTNQALYEWDYARQMLQIRAMSEAGATDD
                     EAVDQLFGPKFLIERPLLDAVRAGDGSVLLVDEIDRADDEFEAFLLELLSDFQVTIPE
                     IGTIVAEQPPVVILTSNRTRELHDALKRRCLYHWVGYPSPEREVEIVLVRAPGVSEVL
                     VAKLVAAVNRLRELELVKPPGVAETIDWARTLDVLGRTELDAGTIEDTVGAVVKERDD
                     LELVKDNLEQIASGA"
     gene            complement(7946..8206)
                     /locus_tag="VH419_07645"
     CDS             complement(7946..8206)
                     /locus_tag="VH419_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043524.1"
                     /translation="MPEQREDAVEVLRAVWAARGQDSSDIDDACGTQKSCKNMEYVSF
                     DGLAEPGSDLVQAWRDRLRREGKLDTSGGSIRDLVLGSSPQP"
     gene            complement(8282..9184)
                     /locus_tag="VH419_07650"
     CDS             complement(8282..9184)
                     /locus_tag="VH419_07650"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HEY7043525.1"
                     /translation="MYEIALSVQSCLRANTAVSVAWVVDTEGLGEVDLTNALALTPGG
                     GRVGSLLAGAVDGQLVENPIGGTGRLVDLQVSDVDALIAGLPSGGHVRCLLVPADRLP
                     GELWDRLVEREPICLVTRVEDGNVGATTLFTRETIHDAPEAAAVMFTRGVSNTAVGAE
                     AVVTVLWPVPTLVVVGGGAIADALAAMAGLLGWHSRVVGGIDDATAVITRLAGIDSLV
                     VMGHEPEDAGPALMAGLASRVGYLGGLGGHRVQQARAEWLAERGITDVSRVHGPAGLD
                     IGAITPAEIALSILAEAVATHSTP"
     gene            complement(9273..9443)
                     /locus_tag="VH419_07655"
     CDS             complement(9273..9443)
                     /locus_tag="VH419_07655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043526.1"
                     /translation="MNDEGPSKRHDEGHDTEAPGVVEQTRDVELPDHRTYSAGVDAWE
                     PLATDPDDIDDH"
BASE COUNT         1563 a         3309 c         3238 g         1534 t
ORIGIN      
        1 ccgaccctct cctacgacac ccaggcggtg ctcgccaccg acaaggttcg cttccagggg
       61 caggaggtgg cggcggtcat cgccgacgac ccgtacatcg cgaaggacgc gtgcgaggcg
      121 atcgtggtcg agtacgaccc gctgccggtc atcgtcaacc cgtaccaggc caccgcccag
      181 ggcgcggcac tgatccgcga tgacaaggac aaccagcacg acaacgtcat ctacgactgg
      241 gaggtcggcg accgcgacgg gactgaccgg gccttcgccg atgccgacgt cgtctccgaa
      301 ctcgagctcc actacccgcg ctcgcatccg tcaccgctgg agtgctgcgg ctcgatcgcc
      361 gacttcaacc gttccacgtc caagctgacc gtctacatga cgacgcaggc accacacatc
      421 atccgggccg cggtcgcgct ggtggccgag ctgccggagc acatgatccg gatcatctca
      481 ccggatctcg gcggcggctt cggcaacaag gtccctgtgt atccgggcta cgtcgtgtcg
      541 atcctcgcct cgatcctgac cgagcgtccg gtcaagtgga tcgaggatcg caccggcaac
      601 ctgatctcca ccggcttcgg ccgtgacgtg tacctgaagg gggagctggc gctgcgccgg
      661 gacggcagga tcctcgctgt ccggatgcac accgacgccg accatggcgc cttcttctcc
      721 gacgcacagc cgagcaagtt caagatcggc ctgatgcact cggccttcgc ctgctacgac
      781 atcccgtacg cgcacctcac tgcacgaggc atgtacacga acaaggcgcc cggcggtgtc
      841 gcctaccggt gttcgttccg gatcacggag gcaatgtact tccaggaacg catggtccag
      901 gctgccgccg acgacctcgg catggaccag gcagcgatcc ggcgcgtcaa cctggtccgc
      961 gacgacgact tcccgcaccg tacgccgttc ggcttcctca ccgattccgg ccagtacgcc
     1021 aagtgtctcg acgtcgggct cgaggcgatc ggctaccagg acttcctgcg ggagaaggag
     1081 gaagccaagc agcacggccg cctgctcggc atcggcatct ccacgatgac agagccgctc
     1141 ggggcgggga acagtcgcga gtacgacatc ctcggcatca agatgttcga ctcagccgag
     1201 cttcgcgtgc acatgacagg caaggcgatc ctccgcaccg gcgcgaagac acaaggtcag
     1261 gggcacgaga ccacgtgggg gcagatcgtg gcccacgagc tgggcatccc cgccgacgac
     1321 gtcgtcgtcg aggagggcga caccgacacc gcacccttcg ggatgggcac gtacgcctcc
     1381 cggagtacgc cggtcgccgg agccgccgtc gcgatggcgt cccgcaagat cagggccaag
     1441 gcccgtcgcc tcgcggctca cctgctcgag gtctcggagg aggacatcga ctgggagctg
     1501 ggccggttct acgtgcggag cgcccccgac cgtggtgtca cgatccagga gtgcgcgctc
     1561 gcggcgtaca gcaacatgcc tgacgggatg gagcccggtc tggaagccaa cgcgtactac
     1621 gacccgccga acctcacctg gccgttcgcc tgctacatcg ccaccgtcga ggtcgatccg
     1681 gacaccggcg tctgggacgt cctcaggacg gtggcggtcg acgactgcgg cgttcgcatc
     1741 aatccgatga tcgtcgaagg ccagatcatg ggcgggctga ccgaggcgta cgccatgtcg
     1801 agcatgcagt acatcacctt cgactccgag ggcaactgcg tcgggtccaa cttcatggac
     1861 tacctgctgc cgacggcgtg ggagacaccg ggatacgaac tgcacgaagt cgtgacgccg
     1921 tgtcctcacc atccgatcgg cgccaagggg gtcggggagt gtgcaaccgt cggcgggccg
     1981 gccgcgttcg tcggggcggt gctcgacgcg atcaaggaca ccggcgtccg caacatcgac
     2041 atgccgctgc tcccggaccg ggtctacgag gcgatgacca ggcggcagga tgtgtccggc
     2101 gcgccgccgc tgaggacgga cacatgagca accgcgccct cgacggatgg gaggtcatcg
     2161 cgcgcgccga cgaactggtc cggggtggcg aggagttcgc cctggcgacg gtcgtgtggc
     2221 ggcagggtcc gtcgtccgga cagcagggtt ctcgtgccat cgtgaacgcc accggcgaga
     2281 tccagggctg gatcggtggt gcctgcgccg aaccagtcgt gatcagggaa gcccagcgcg
     2341 tcatccgcga tcgcgagccg aagctcctcc tgctgggcaa ccccggccag ttcggtgggg
     2401 cggtacccga tgggatcacc gtcatcccga tcgcctgcca gagcgaagga gccctcgagg
     2461 tgtacctcga acctgtcgtc ccgaccactc atctggtggt cgtcgggcga tcgcccatgg
     2521 cgcacacgtt ggccgacttc gcgcgtgggc tcggctggcg ctgcgacctt cgtgaggtcg
     2581 ccgacttctc ggccgccgat gtcggggcga cttcggtcgt cgtgatcgcc acgcaggggc
     2641 atggcgacga agaggccctc gaggaggcag tctccgcgtc gccggctttc gtggggctgg
     2701 tggcgtcgcg ccggcgcggc gagacagtgc tcggatacct cgctgacaaa ggcctcgccc
     2761 gggagcagct cgaccgggtg aaggtgccag tcgggctgga tctcggccgc acctcacatc
     2821 gcgagatcgc ggtctcgatc cttgccgagc tcgtccagct gcgcgcttcg ggagcgctgc
     2881 gcgaggacgg cgcagaggac cagcacggcg tacgccagga gtcagcgagc acagccctgg
     2941 cggtcgaccc ggtatgtggc atgaccgtgg ctgccgatcc gtcgagccgc ccggtcgagc
     3001 acgccggcac gacctactac ttctgcagtg caggttgccg tgccgctttc gagaaggatc
     3061 cgcagaccta catcaccggc gtcagcgccg gctaggagat cggcatgctg ctgacgaacg
     3121 cgttcgaggt cgaccagcct gtcgacaagg tgtgggagtt cttcaacgac attccgcagg
     3181 tcgcggcatg tctcccgggg gcggagctca ccgaggatct cggcgacggc acgtacgccg
     3241 gccgggtcgc ggtacggatg ggaccggtgc ggctgtcctt caacgggacc gcggacatcg
     3301 tcgagcgcga cgaagcggcc aagcgcatcg tcgtcgatgc cgccggggcg gacgagaagg
     3361 ggcgcgggca ggcaagcgtc ctggtgacag cggcactggc cgccaagggt cgcgggacca
     3421 aggtcgacct gcagcaggat ctgcagctgt caggggcggc ggcgcagtac ggccgcggca
     3481 tgatctccga cgtcacgacg gtgctgatgg gccagttcgc gaacaacatg cagcaccgga
     3541 tcgctgcggt ggacgccggc gagacgccgc agcaggcatc ggcaccggcg gctgcgagcg
     3601 gtttcagcat cggctggcag gcgatgcggc tcgccctggc acgggtgttc cgtcggttct
     3661 ttcttccgta ccaaccgcgt cagagctgag gaggtcggca atgtcgggca tggtcttgtg
     3721 gtgggtcggc aacgtgctct tgctcgtggt cgtggtcccg atcctcgtcg cgctgctcaa
     3781 ccgcgtgctc gctgcgctgg agcgcatccg gggcgcgacc gacgacatcc tggcgggtgg
     3841 tgtcgagctg gccggccagc tcgagggtgt tcccgaagga ctggccacga ccgaccgcac
     3901 cgtgcacgag atcgccgtcg gtgcgacccg gtacgccggt tcggttgcga agctgctggg
     3961 ctgaggaggg agctgccatg cctgtcgctg cctgggtgac catcgtcatc agtgtcctga
     4021 tcatcgcggc tgctgcactc ggtctgatca gggtgatctt ccacctccga gcaaccaacc
     4081 gtgcgttgga gggcgtggtg ggtggcgtgc gtgtcgtcgc gcaccagacc cggaccgtgc
     4141 ccgaggtgat cccgtccgtc aacgacaacc tgaagccggt ccgcgacttc tgcgagacgg
     4201 tctgaagggg tgacgatgat gttgatgaca tcccacgcac gctcggtcgc gatcagtgac
     4261 acgacgggct gggtgatcgg ctacacggcg gcgatcgtgg tcgtgctcgt ggtcgtggcc
     4321 ctcgtcgtgc cgatcctgct gctggcgcac tcgatcggca gcaaggcgaa gctgatcgac
     4381 gactccctgc aggactccgt gcgtcatact tccgccctca gcgggctcaa cacgacgatc
     4441 gaccacgtca ccgtgatcgt cgcagggctc aaccgcggac gcaccaggtt gggggggtga
     4501 cgatgcgcga cgtgactgtg gtggctctga cgtcgacgaa cgagaacctg tggtggggcg
     4561 cggtcgtcgg aggattcgtg gtcgtgcttg ccgtggcggc cttgctcacg attctggtgc
     4621 tcgaggtgcg cacgatcgac cgtcgtgtcg tccaggtgcg cgacacgttg caggcggccg
     4681 ccgacaacac cgccgacact gccctgatcg caacgaccgc cgacggggtc gaggcagtgc
     4741 tcgccgaagg cctgcagcac catctgttcc tcggacgcgt gttgggcaag gtgagaatgt
     4801 gaccgcgctc gtcgtgttgt cggtcatcga catcgccttg ctgatcgccg ggttggcgtt
     4861 ctacctgtac gtcgtcggtt cccagctgac gcggatcgcg acaaacctcg aggagtgcca
     4921 ggcgctggtc gcgcagatcg tccgcaacgc cgagccgatc atccccgggg tgggccacat
     4981 caaccgcacc ggtggcgtcg tcgcaggtgc gctgccactc ctctacgaca tggccgaggc
     5041 gatcgtcacc ggggtggccc cggcgcccga gcagcccgcc gagcgtccac cagcagcccc
     5101 cgcatccgga cgacggcggt cgaggctgca cgacagcgtc ggctacgcgc cgactcgttc
     5161 gtcatgaccc accggtcggc tggccgggtt ctcgacctgt ggtgagggga cctgtggtcg
     5221 gggcgacgta cgcgccgatc cactcctcct tccacgaccg ctcgaactgt tgctcgttgc
     5281 agcgtgggtg gtagatcgca gccagctcct gcgtgatctg ttcacggtgg gaccggaggc
     5341 ctcccgggac ctcgtaggtg cagatgtaga cctgccagcg gctgcctgcc cggcgtaccc
     5401 agcaaggggc gcctggatgg ctgaagggaa atcgctcgct cgagagatcc tcggagtgac
     5461 ctacgtagat caccgcgtag tggtcgggct tgccgtccgg gtcgggccgg tagacgacgg
     5521 cgtacaccgc agccgcctgc ggcggggtcc agccggccag cagccgcggc ccctcgaagg
     5581 gatacccggc cagtgatccc agcctgatca cgggaccgac ccaccccgcc gatcgaaccc
     5641 gtcgagatca agcggtgact cgctcacggc ctccgcgctc gcgatcggcc acgccggcag
     5701 ccccgcccgc tcggcagccc gtacgaactc cgccttgccc tgctccagcg cagcctggag
     5761 gtcgtcggcc tccacgatca gctgtgcgcc gtagcgactc gacccgatac ccgaggcgat
     5821 cccgccggat gccgccaccg cgtcagcgag ctcgaccacc tcctcacgag cgagtactcg
     5881 gtcaccttcg gcctggatcg tgacgctcca ccgcacggtt cggaccctag ctcagctcgg
     5941 gcagcctggc ggcgaactcg gccaggctgc gcagatcgtg gccggagtac agcacgtcga
     6001 tgtgcggagc ggccaccatc atgccgaggg tgctgggctg gaagtcgatg tcgtctccct
     6061 tgtgcgggtt catccagacg acccgatgag acagccgcga gagccgttcc atcgcagctg
     6121 ccagcacacc ggggtcaccc cggtccagtc cgtcggagca gatgacgacg atgccgcctc
     6181 gacacactcc tcgccggccc cagtcccgca cgaatgcgtc cagggacgcg ccgatccggg
     6241 ttccgccgtc ccagtcgacg accgccttgg cggcccggtc gagcgcctcg tccggcttgc
     6301 ggtgctcgag ctcgcgggtg atccgggtga gccgcgtgcc gaagcagaac acctcgacac
     6361 gggcggtcac gcgtctcgtg gagtacgcga actggagcag gttgcgggag tagtccgcca
     6421 tcgaacccga gacgtcgagg atcaggatca acgggcgagg ccgcagccga cgtcgacgcc
     6481 agtacaacga ggacggctcg ccgtgcatcc gcatcgtctc gcggatcgtg cggcgcatgt
     6541 ccggccggac gccgctgccg gccggtgcgg tacgccgagt ccgtcgccgc ggtggactca
     6601 gccgcatcgc cctcatgatc cggtgcagcg cctccagctc ttccgtcgta catgcggcaa
     6661 aggacttgga gcgcaacgtc gccacgtcgg acgccaccag cccgagccga gcctcccggt
     6721 cctcgcggga cacgcccccc ggctcggtct ccggcacctg caacacggca tccgccgcgg
     6781 acctggcttc aggactgtag ggcttggact ccgtcggtgc gtcctccccg aggaagaagc
     6841 ggcgaaacac ccggtcgtag acgtcgatct cgtctcggcg cgcgacgagt gcggctcggc
     6901 cggcccagta cacatcgagc aggtcgctcg gatcgagcgc ggcgagcgag gcgctgtacg
     6961 tcagtacgtc gccggagccg accgacaacc ccgcctctcg gagttcgccg ccgaaggcga
     7021 ccagcgtctc gacgaatgga tgggagtcgt caggcaccgg aggcgatctg ttcgaggttg
     7081 tccttcacga gctccaggtc gtcgcgttcc ttgacgacgg caccgaccgt gtcctcgatg
     7141 gtgccggcat cgagctcggt tctgccgagg acgtcgaggg tgcgcgccca gtcgatcgtc
     7201 tcggccactc ccggtggctt caccagctcg agctcccgaa gccggttcac cgcggccacg
     7261 agtttggcga cgagcacctc ggacactcct ggtgcacgga cgaggacgat ctccacctca
     7321 cgctcggggg atgggtaacc aacccagtgg tacaggcacc gccgcttcag cgcgtcgtgc
     7381 agctcgcgcg ttcggttgga cgtgaggatg acgacaggcg gctgctcggc gacgatcgtg
     7441 ccgatctccg ggatcgtgac ctggaagtcc gagagcagct cgaggagaaa cgcctcgaac
     7501 tcgtcgtcgg cacggtcgat ctcgtcgacc agcaggacag agccgtcccc ggcccggacg
     7561 gcatcgagca gcggacgctc gatgaggaac ttcggaccga acaactggtc gacggcctcg
     7621 tcgtcggtgg ccccggcctc agacattgcc ctgatctgca gcatctggcg ggcgtagtcc
     7681 cattcgtaga gagcctggtt ggtgtcgatg ccttcgtagc actggagccg gatgagccga
     7741 cggccggtcg tcgcggcgag gaccttcgcc acctcggtct tgccgacccc gacctctcct
     7801 tcgagcaaca gcggccgttg cagtgacaga gcgacgaaca gcgccgtcga aagcccgcga
     7861 tcagccagat agccgccgcc tcggagggtc tgtgtcagat cgccgacggt ctgagcacgg
     7921 tccatggacg ctcctcagtg catcgtcatg gctgcggcga cgagccgaga accaggtccc
     7981 ggatgctccc cccggacgtg tcgagcttgc cctcgcgtcg aagccggtca cgccacgcct
     8041 ggacgagatc actccccggc tccgccagac cgtcgaagct gacgtactcc atgttcttgc
     8101 acgacttctg cgtgccgcag gcgtcgtcga tgtcggagct gtcctggcct cgggcagccc
     8161 acactgctcg caagacctca actgcgtcct cgcgctgttc cggcataccc ggactcctgt
     8221 cggtgtcgac ctcgtaccca gtctatccgg gtccggaagg ctttcccgtt gcgccaggac
     8281 actatggcgt ggagtgcgtg gccacggctt cggcgaggat cgagagcgcg atctcggccg
     8341 gcgtgatcgc gccgatgtcg aggcccgccg ggccgtggac ccggctgacg tcggtgatgc
     8401 cgcgttcggc gagccactcc gcccgcgcct gctggacgcg atgaccgccc aacccaccga
     8461 ggtagcccac gcggctcgcg agcccggcca tcagggcagg accagcgtcc tcgggctcat
     8521 gacccatcac cacgagcgag tcgatgcccg cgagcctcgt gatgaccgcc gtcgcatcgt
     8581 cgatgccgcc gaccacgcgg ctgtgccaac ccaacaggcc ggccatggcc gccaacgcgt
     8641 ccgcgatcgc gccacctccg acgaccacga gcgtgggaac cggccagaga accgtcacga
     8701 ctgcttccgc acccacggcg gtgttgctga caccacgcgt gaacatcaca gcggccgcct
     8761 caggagcgtc gtggatcgtc tcccgcgtga acaacgtcgt cgcgccgaca ttcccgtcct
     8821 cgacgcgcgt gacgagacag atcggctcgc gctcgacgag ccgatcccac agctcgccgg
     8881 gtagccgatc ggccggcacg aggaggcacc gcacgtgccc gcccgagggc agcccggcga
     8941 tgagggcgtc gacgtcgctc acctgcaggt cgaccaaccg gccggtgccg ccgatggggt
     9001 tctcgacgag ctggccatcg accgctccgg ccagcaacga acccacgcgg ccaccgccag
     9061 gagtcaacgc caacgcgttc gtgaggtcga cctcaccgag cccttccgtg tcgaccaccc
     9121 aggccacgct gaccgccgtg ttcgcccgta gacacgactg gacgctgagc gcgatctcgt
     9181 acatgacgac gagcctagga tcgccggcgg ttctggtcag ccctggcgta cgccgccaca
     9241 catgacgttg tggaaccgac gatctgcgtc agtcaatggt cgtcgatgtc gtcgggatcg
     9301 gtcgccagcg gctcccaggc gtcgacgccc gcggagtacg tgcggtggtc ggggagctcg
     9361 acatccctgg tctgttcgac cacgcccggc gcttcggtgt catggccctc gtcgtgtcgc
     9421 ttggacggtc cctcgtcgtt catcgagtca ctccctcgcc aaccgtgatc cggcccgcga
     9481 cgtacccacg gctggtgcac gcgaaacagc ccagccccac agtgctttgc tggttcacgt
     9541 tgatgcgcta gtgtcccgcg tcggaggttc cttcacaggt gcggcgaggg caggtggatg
     9601 catcgcaagg cggaggaggg aggcgatgcg cagcatcgtc gacc
//
LOCUS       DATBFZ010000246         2472 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_1470967, whole genome shotgun sequence.
ACCESSION   DATBFZ010000246 DATBFZ010000000
VERSION     DATBFZ010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2472
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1470967"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..738)
                     /locus_tag="VH419_07660"
     CDS             complement(<1..738)
                     /locus_tag="VH419_07660"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY7043527.1"
                     /translation="MVHGVVGREAELARLEGFLDRMVEGPTALVLEGEAGIGKTTLFD
                     ATTGAATARGYRVLAARAAQPEARLSFAAFTDLLDSVAPEVLPNLPDPQRRAVEIALL
                     RLDPGGRPPDQRTVCAGFVSAIATLASDTPVLIAVDDVQWLDRPSARVLEFAARRLGD
                     RRVGLLLSARASDRSELPFGLSRALPDRRVDRLRVGPVSLAVLHHVIRERLDVSFLRP
                     QMLRIERASGGNPFFALELARDIAKRGT"
     gene            complement(749..970)
                     /locus_tag="VH419_07665"
     CDS             complement(749..970)
                     /locus_tag="VH419_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008362043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEY7043528.1"
                     /translation="MAEPVGVRVGDEGIRLGQFLKLANAVDQGSDAKALLAGGFVRVN
                     GEVEKRRGRQLVVGDVVQLAGSSYVVTNG"
     gene            complement(963..1187)
                     /locus_tag="VH419_07670"
     CDS             complement(963..1187)
                     /locus_tag="VH419_07670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043529.1"
                     /translation="MRAFRVVWTFLVDLIIGDDPKVAVAVVGSLVAAAVALEFGVADS
                     VVAVGGALLVMLAFTVCLLLDASRSRRVDG"
     gene            complement(1184..1972)
                     /locus_tag="VH419_07675"
     CDS             complement(1184..1972)
                     /locus_tag="VH419_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020576344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043530.1"
                     /translation="MTAADLGLLASTFVASFVECVEALTIVLAMGITRGWRAAIAGTV
                     TAVVALAVFTAVAGYALTRWLPTALLQLVVGALLLIFGLQWLRKAILRSAGLKSLHDE
                     SAEYAERTHAGQAASDRTIMGLDAFGYIVSLKGVFLEGVEVVFIVITFGLNAGNVPLA
                     AVAGGTAALVVLTLGLLVHRPLAAVPENALKYGVGLLLTTFGTFWSLEGLGILSEGHD
                     SLSRPAEDWALLVLLAVWFGWSRLVISWLRRRVDEPSESEVMAA"
     gene            complement(2018..>2472)
                     /locus_tag="VH419_07680"
     CDS             complement(2018..>2472)
                     /locus_tag="VH419_07680"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY7043531.1"
                     /translation="SAEVIRLRRRGSPQVEARLEPVRLRADQHQLSRLVRNLVDNATR
                     HATSTVSLVLRNGDGTAVLHVGDDGPGVPEHEAERIFERFVRLDESRGRATGGTGLGL
                     SIAREIARRHGGDVTVTSREGSSTQPTGALFVVRLPVRPPSCQNPDDL"
BASE COUNT          444 a          901 c          807 g          320 t
ORIGIN      
        1 ggttccccgc ttagcgatgt cgcgggcgag ctccagcgcg aagaacgggt tccctccgga
       61 tgcccgctcg atgcgcagca tctgtggtct gaggaaggac acatcgagcc gctctcggat
      121 gacgtgatgg agcacggcca agctgaccgg ccccacccgg agccggtcca ccctgcgatc
      181 cggcagcgcg cggctcagcc caaagggcag ttcgcttcgg tccgaggctc gcgccgagag
      241 gagcagcccg acccgtcgat cccccagccg ccgcgccgcg aactccaaga cgcgggcaga
      301 cgggcgatcc agccactgga cgtcgtcgac cgcgatcagg accggagtgt ccgaggccag
      361 cgtggcgatc gcggacacga acccggcgca gaccgtgcgc tggtccggag gtctgccccc
      421 ggggtcgagt cggaggaggg cgatctccac cgccctccgc tgggggtcgg gcaggttggg
      481 cagcacctca ggggcaacgg agtcgaggag gtcggtgaac gcggcgaagg acagcctggc
      541 ctctggctgt gccgcccggg cggcaaggac ccgataaccg cgggccgttg ccgcccccgt
      601 cgtcgcgtcg aacaacgtgg tcttgccgat ccctgcctca ccctcgagaa ccagagccgt
      661 gggcccctcg accatcctgt ccaggaagcc ttcgagccgg gcgagctctg cctcccgtcc
      721 gacgaccccg tggaccaccg gaccccctct agccgttcgt gacgacgtac gacgaaccgg
      781 cgagctgcac gacgtcacca accacgagct gacgaccacg acgcttctcg acctcgccgt
      841 tcacccggac gaaaccgccc gccaacaggg ctttcgcgtc gctcccctgg tcgacggcgt
      901 tcgcgagctt gaggaactgg ccgaggcgga tcccttcatc tccgacgcgc acgccgaccg
      961 gctcagccat cgaccctcct cgacctcgag gcgtccagca gcaggcacac cgtgaacgcc
     1021 agcatgacga gcaacgcacc accgaccgcc acgacactgt ccgcaacgcc gaactcgagt
     1081 gccaccgcgg ccgcgaccaa cgacccgaca accgccacgg cgaccttcgg gtcgtcgccg
     1141 atgatgaggt cgaccaggaa ggtccagacg acacgaaacg ccctcacgcc gccatcacct
     1201 ccgactccga cggctcgtcg acccgtcgac gcagccacga gatcaccagt cgcgaccagc
     1261 cgaaccagac ggccagcagc accagcagcg cccagtcctc ggcgggccgg gacagactgt
     1321 cgtgaccctc ggagagaatc ccgagacctt ccagcgacca gaacgtgccg aacgtcgtga
     1381 gcagcaggcc aacgccgtac ttcagcgcgt tctccggcac agccgccaac ggccggtgca
     1441 cgagaaggcc gagcgtcagc acaacgagag cagccgtgcc gccggccacg gcggcaagcg
     1501 gcacgttccc cgcgttgagg ccgaacgtga tcacgatgaa gacgacttcg acgccctcga
     1561 ggaagacgcc cttcagcgag acgatgtagc cgaacgcgtc gagacccatg atcgtgcggt
     1621 cggaggcggc ttgcccggca tgcgtccgct cggcgtactc cgcggactcg tcgtgcagcg
     1681 acttcagccc ggccgagcgc aggatcgcct tgcgtagcca ctggaggccg aagatcagca
     1741 acagcgcgcc gaccacgagc tggagcagag ctgtgggaag ccatcgggtc agcgcatagc
     1801 cggcaacagc ggtgaacacg gccagcgcca cgacggcggt gacggtgccg gcgatggccg
     1861 cccgccagcc gcgcgtgatg cccatcgcca acacgatcgt gagcgcctcg acgcactcga
     1921 cgaaggaagc gacgaacgtg gaggcgagca accccaagtc ggctgctgtc acgtcggcca
     1981 gcctacggct ccgccgagtt gtcagaagta catcgagcta taggtcgtct gggttctgac
     2041 aactcggcgg acggaccgga agacgtacga cgaagagcgc gccggtgggt tgggtgctgc
     2101 tgccctcgcg ggaggtgacg gtgacgtccc caccatgtcg gcgggcgatc tcacgggcga
     2161 tggacaggcc gagtccggtt ccaccggtcg cccgtcccct gctctcgtcc agccgcacga
     2221 accgctcgaa gatgcgctcg gcttcgtgct ccggcacacc ggggccgtcg tcgcccacgt
     2281 ggagcaccgc tgttccgtct ccgttgcgga gcaccagcga caccgtcgag gtcgcgtgcc
     2341 gggtggcgtt gtcgaccagg ttgcggacga gccggctcag ctggtgctgg tccgccctca
     2401 ggcgcaccgg ctcgagccgg gcctcgacct gcgggcttcc ccgtcgacga agccggatca
     2461 cctccgcgct ca
//
LOCUS       DATBFZ010000247         2020 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_491766, whole genome shotgun sequence.
ACCESSION   DATBFZ010000247 DATBFZ010000000
VERSION     DATBFZ010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491766"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(320..1708)
                     /locus_tag="VH419_07685"
     CDS             complement(320..1708)
                     /locus_tag="VH419_07685"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020392855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEY7043532.1"
                     /translation="MSALHEVKDWIAEQLPRLIEKYDVPGAAVAVLADGEVVDDAAGT
                     LSKATGVEATADSVFQIGSITKLWTSTLVMQLVDEGKVDLDVPVRTYLPEFRIKDDSA
                     AAQITTRQLLTHTAGFEGDIFTDTGLGDDCVEKYLGVLHDVPQLFAPGEQFSYNNAGY
                     CVLGRLVEVLREKPYDACLREHLIAPLGLTHTATSAYEAIMFRAAVGHVENEPGAGYE
                     PAPVWALARSNAPAGSMLTMRPRDLVAFAQMHLDDGKAPDGTAVLAAGTAARMHERQV
                     DLPDLGLMGSSWGLGFERFDTAQGAIIGHDGGTIGQSAFLRIIPEAGVAVALLTNGGD
                     VASLYRDIVGHVIEELTGNALPALPVPPADPERIDASRYVGTYSVEVADLTVSQDEDG
                     RIWIEQTPKGIFEELGEKPERKELVHYKGDSLIPSQPEKGMHMPHVFVGDDGDGHALY
                     LHVGRAIRRAGA"
BASE COUNT          289 a          675 c          713 g          343 t
ORIGIN      
        1 gagccgaggg ccatggtgaa tgtcgcaccg tcgacgaact gctcgtcgcc acctcccccg
       61 ccgccgctgt tggagtcgtc gccgccaccg cagccggtga ggacggcggc ggtgacgcag
      121 aggccgagga cggtcgcggt cagcttcatg gggttcctgt tcacagtcat gatcgagggt
      181 ggtcgccgtg cccgctgatc gagcttgtcg cgatcaaggc actcgacctg atcgcgtgtc
      241 cgctgatcgc gtgtccgctg atcgagcttg tcgagatcaa ggcactcgac ctgatcgcgt
      301 gtccggtggt cacagtctcc taggctcccg ctcgccggat cgcacggccg acgtggaggt
      361 agagggcgtg cccgtcaccg tcgtcaccga cgaagacgtg cggcatgtgc atgcccttct
      421 ctggctggct cgggatcagg ctgtccccct tgtagtgcac gagctccttg cgttcgggct
      481 tctcgccgag ctcttcgaag atgcccttcg gtgtctgctc gatccagatg cggccgtcct
      541 cgtcctggct gacggtgagg tcggcgacct cgacggagta cgtgccgacg taccggctgg
      601 cgtcgatccg ctcggggtcg gccggcggga ccggaagcgc gggaagcgcg ttgccggtga
      661 gctcctcgat cacgtggccg acgatgtcgc ggtacagcga ggcgacgtcg ccgccgttgg
      721 tcagcagcgc cacagcgacg ccggcctccg gaatgatccg caggaaggcg gactggccga
      781 tcgtgccgcc gtcgtggccg atgatcgcac cctgcgcagt gtcgaaccgc tcgaagccca
      841 gcccccacga gctgcccatc agaccgaggt cgggcaggtc gacctgacgc tcatgcatgc
      901 gtgcggcggt gccggcggcg aggacggcgg tgccgtcggg cgccttgccg tcgtcgaggt
      961 gcatctgcgc gaacgcgacc aggtcgcgcg gacgcatggt gagcatggag ccggcaggcg
     1021 cgttcgaccg ggccagtgcc cataccggcg cgggctcgta gccggcgccg ggctcgttct
     1081 cgacgtgccc gaccgcggcg cggaacatga tcgcttcgta cgcgctcgtc gccgtgtggg
     1141 tgaggccgag gggcgcgatg aggtgctcgc gcaggcaggc gtcgtacggc ttctcgcgca
     1201 gcacctcgac gagccggccg agcacgcagt agccggcgtt gttgtaggag aactgctccc
     1261 cgggcgcgaa cagctgtggg acgtcgtgca ggacgccgag gtacttctcg acgcagtcgt
     1321 cgccgaggcc ggtgtcggtg aagatgtcgc cctcgaagcc ggcggtgtgc gtgaggagct
     1381 ggcgcgtggt gatctgtgcg gccgcgctgt cgtccttgat ccggaactcg gggaggtacg
     1441 tgcggaccgg gacgtcgagg tcgaccttgc cctcgtccac cagctgcatc accagcgtgc
     1501 tcgtccacaa cttggtgatc gagccgatct ggaagacgga gtcggccgtc gcctccactc
     1561 ccgtggcctt gctcagggtg ccggcggcgt cgtcgacgac ctcaccatcc gccagtacgg
     1621 cgaccgccgc cccagggacg tcgtacttct cgatcagcct gggcagctgc tcggcgatcc
     1681 agtccttcac ctcgtgcaga gcgctcatgg cgctgatcgt aagaacgccc cgacgccgat
     1741 tgttcgtcgg gcccgacaag atgggcctgc tgtctccgtc ctcccgcaca acccgctgga
     1801 tgagccgacc cgctggtcga gcctgtcgag acccaggtcg ccggccgagc cgacccgctg
     1861 gtcgagcctg tcgagaccaa ggggcgtgcc cgacgatgag cttgccgtcg ccggatctcc
     1921 gggtgacccg atgttgtggt tacccggggg cgagagcccc gacgtgaacc gtcgacgccg
     1981 acagccagaa cacgtcggga cggcgattca ggctgcgtcc
//
LOCUS       DATBFZ010000248         2520 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_4097419, whole genome shotgun sequence.
ACCESSION   DATBFZ010000248 DATBFZ010000000
VERSION     DATBFZ010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4097419"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="VH419_07690"
     CDS             <1..355
                     /locus_tag="VH419_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020386881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEY7043533.1"
                     /translation="GQYLSISSKASDAQKQIALNYFKTGVLTDAEVAEWIKTGSVPIV
                     KSAASQLSSAGADAPFLQFVYDISSKAKTFSQSWDQALSPTQAEVLLDNISKLFGLSV
                     SPQQFANNLNAVIGK"
     gene            433..1278
                     /locus_tag="VH419_07695"
     CDS             433..1278
                     /locus_tag="VH419_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012921818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEY7043534.1"
                     /translation="MALPALAFFILFGLVPLVGVVLMSFTTWDGIGTIHPSGLTSWKA
                     VLKQPGLPHAIYVTFLLMALSWLFQTPMSILLGVFMAGRQRYRAFLAVFFFIPLVLSS
                     AAIAITFKALLDPNFGLGAGLHADWLSQDWLGDPKLAVATVVFVVSWQFIPFHTLIYQ
                     GAVRQIPQSMYEAAELDGAGRVRKFFSITLPQLKYTIITSSTLMVVGSVTFFDLLFVL
                     TQGGPADATTNLALMMYKLGFQANLMGQACAIACMLVVLGLALALLLQRLGGRDRTAS
                     QMEGA"
     gene            1280..2173
                     /locus_tag="VH419_07700"
     CDS             1280..2173
                     /locus_tag="VH419_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012921819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEY7043535.1"
                     /translation="MATATIEARRATARPSRGRKSVPRRLAGWNWIGGAGAWIWFFIV
                     MLPIYWIVITSFKTQNNYFATNPLVPPHNPTLENYRLVLKSDFVQYFVNTVIVTAGAV
                     IPAVAVSFMAAYGIIRDHRRWFLRSTNSMFLMGLAIPVQATIIPVYLLIIKMKLYDSL
                     LALILPQIAFAIPLSVLILANFIRDVPNELFESMRVDGANEWAMLWRLAFPLTRPAIV
                     TVTIYNALTVWNNFLLPLVLTQSPDKRTLPLALWTFQGQYSINVPAVLASVLLTALPI
                     LLLYIIGRRQMLSGLSAGFTK"
     gene            2198..>2520
                     /locus_tag="VH419_07705"
     CDS             2198..>2520
                     /locus_tag="VH419_07705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043536.1"
                     /translation="MRARSIAAVVAAALVIAGCAASTTSSTSHAPSTPSAPPGPQPSR
                     AAVRVAPQAASATAARTRPRPVIVVDPGHSPAIFAIDRRTGLNVSDYENEPEMRDVFN
                     VAVRVA"
BASE COUNT          388 a          861 c          795 g          476 t
ORIGIN      
        1 gggtcagtac ctgtcgatct cgtccaaggc gtcggacgcg cagaagcaga tcgcgctcaa
       61 ctacttcaag accggcgtgc tcaccgacgc cgaggtggct gagtggatca agacgggaag
      121 cgtgccgatc gtcaagtccg cagccagcca gctcagctcg gcaggtgccg acgcgccctt
      181 cctgcagttc gtctacgaca tctccagcaa ggcgaagacg ttctcgcagt cctgggacca
      241 ggcgctgagc cccacgcagg ccgaggtgct gctcgacaac atttccaagt tgttcgggct
      301 ctccgtctcg ccgcagcagt tcgccaacaa ccttaatgct gtaatcggga aatgactgcg
      361 gtcgcacccc cgctccaaca atctgcgtcg ccggccccca cgcggggccg gcggcagcgt
      421 tcgctgagtt ggttggcgct tcccgcgctg gcgttcttca ttctcttcgg gctggtgccg
      481 ctcgtcggcg tggtgttgat gagcttcacc acctgggacg gcatcggcac catccacccg
      541 tccggcctca ccagctggaa ggcggtgctg aaacagcccg gcctgccgca cgcgatttac
      601 gtgacgttcc tcctcatggc tctgtcctgg ctgttccaga ccccgatgag catcctgctc
      661 ggcgtcttca tggccggccg ccagcgctat cgcgcgttcc tggccgtctt cttcttcatc
      721 ccgctggtgc tgagctcggc cgcgatcgcg atcaccttca aggcactgct cgaccccaac
      781 ttcgggctcg gcgcggggct gcacgcggat tggctgtccc aggactggct aggcgacccg
      841 aagcttgcgg tcgcgaccgt ggtgttcgtg gtgtcgtggc aattcatccc gttccacacg
      901 ctgatctatc agggcgcggt gcggcagatc ccgcagtcga tgtatgaggc ggccgagctc
      961 gacggcgcag ggcgggtccg gaagttcttc agcatcacgc ttccgcaact gaagtacacg
     1021 atcatcacct cgtcgacgct gatggtcgtc ggctcggtca cgttcttcga tctgctcttc
     1081 gtgctcaccc agggcgggcc ggcggacgcc acgaccaacc tggctctgat gatgtacaag
     1141 ctcgggttcc aggccaacct catgggccag gcgtgcgcga tcgcctgcat gctcgtggtg
     1201 ctgggtctcg cgctggccct cctgctgcag cgcctgggcg gtcgtgacag gaccgcgagc
     1261 cagatggaag gggcctgagg tggcgaccgc gaccatcgag gcgcgtcgag cgacggcccg
     1321 gccgtcccgt gggcgcaagt ccgtcccgcg gcgcctggcc gggtggaact ggatcggcgg
     1381 ggcgggggcg tggatctggt tcttcatcgt gatgctgccg atctactgga tcgtgatcac
     1441 gagcttcaag acgcagaaca actacttcgc cacgaacccg ctggtgccgc cgcacaatcc
     1501 gacgctggag aactaccggc tagtcctgaa gtcggacttc gtccagtact tcgtcaacac
     1561 ggtcatcgtc accgcgggcg cggtgatccc ggcagtggcg gtgtcgttca tggccgccta
     1621 cggcatcatc cgcgaccatc ggcgctggtt cctgcgctcg accaactcga tgttcctgat
     1681 gggcctggcg atcccggtgc aggcaacgat catcccggtc tacctgctga tcatcaagat
     1741 gaagctctac gacagcctgc tcgccctcat cctgccccag atcgcgttcg cgatcccgct
     1801 ttccgtgctg atactcgcga acttcatccg ggacgtcccc aacgaactgt tcgagtccat
     1861 gcgcgtcgac ggcgccaacg aatgggcgat gctgtggcga ctcgccttcc cgctgacccg
     1921 tccggcgatc gtcacggtca cgatctacaa cgcgctgacg gtatggaaca acttcctgct
     1981 gccgctggtg ctgacgcaga gcccggacaa gcgcactctg ccattggcgc tgtggacgtt
     2041 ccaggggcag tacagcatca acgtgccggc cgtgctggcg tcggtgctgc tgacggcgct
     2101 gcccatcctg ctgctctaca tcatcggacg gcggcagatg ctcagcggtc tgagcgccgg
     2161 gttcaccaag tagttttcgc gccgctacgg taggccggtg cgcgcacgct cgatagccgc
     2221 ggtggtcgcg gcagccctcg ttatcgcagg gtgtgccgcg tcgacgacgt catcgacttc
     2281 ccacgcgccg tcgactccgt ccgccccgcc gggcccccag ccgtcgcgcg ccgcggtccg
     2341 ggtcgcgccc caggcagcat cggcgacagc ggctcgcacc cggccgcgac cggtgatcgt
     2401 cgtcgatccg gggcacagtc cggcgatctt cgcgatcgac cgccgcaccg ggctgaacgt
     2461 gtccgactac gagaacgagc cggagatgcg cgacgtgttc aacgtcgcgg tacgggtggc
//
LOCUS       DATBFZ010000249         6926 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_4100729, whole genome shotgun sequence.
ACCESSION   DATBFZ010000249 DATBFZ010000000
VERSION     DATBFZ010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 6926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6926
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4100729"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1807)
                     /locus_tag="VH419_07710"
     CDS             complement(<1..1807)
                     /locus_tag="VH419_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_263577181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HEY7043537.1"
                     /translation="MRGPIEGPLGRHRSRVALAVFAVLLAGLTNVARTEAQPPPPTFP
                     MALDATALTLDAWILTGFTPGASQPGQLPNNIIQNMDLSPGLYEMIIGSGNAMNCKLE
                     VTATGTWDYSVFFPDPNFPTTCDGFIQGRGTNHLVITGYTVFVDATALTTTQFIHTNL
                     LFNTGHNTWNSKVLNTNTNTWEPNVVQFQLVPAPLQGLLMQAGFLCCYFEVLLDGTVR
                     FPTQFPSGEPTGFETFTRTDTRPCPSNSTLPCSTANNTATVLGKTIQIDATALGAGPP
                     SGGVPFGFHLFSLLTPEPSPFRQDQVQTVTIPPMPLKCDTCYVQFFSEFSFNTNQIVS
                     FPWKLLNNGTVDYDVAIPCVGGRGTTRLTVFCDHDQDGDGVRDAVDNCPTTPNPDQAD
                     TDGDRVGDACDNCPLRANSNQADNVCALDTGETLAAPPSKPQGEPVLVTAKFKNNTAS
                     PMRTIRPDCVNTTFTVMDGGLQLDPIIREKMYGIPDDLVTIGPGAEFAVTCDLTEIYD
                     ATFLTPGTYEVTATYGNQFVDRNIVQGTCTLPGGVGCISDIWIGAVTSIPQTVAITPP
                     SSGVPPIRVEIDIEPLISKNLWPCGLKFTIPVAVLS"
     gene            complement(1809..2375)
                     /locus_tag="VH419_07715"
     CDS             complement(1809..2375)
                     /locus_tag="VH419_07715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043538.1"
                     /translation="MTRILRALSVLAVATIVVAAVPAAAWAESNIPPGEALPFQDLIN
                     PQAKGKKLTGFLTIAYELGEVPEDQLDFCSSIFINNMFVVTTLEHRGDVRPFNSDFSQ
                     TGTQPFCFDDTQGQIDVVNKLLSRAVASFFDPTATPITPPPGFACPAKPSQLCYQVKS
                     VKEFLGTGTGAASMEIKLAVKPGPDESD"
     gene            complement(2438..3310)
                     /locus_tag="VH419_07720"
     CDS             complement(2438..3310)
                     /locus_tag="VH419_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043539.1"
                     /translation="MTNRQRGPGWLYGALGGLVVLLAAIAVTVSPEPAWAVVDCTNIA
                     PGSTADTDGDGFTDAQECAGLNLDLGTTRTRLEPCGSPNAGAECVDPNKKDLFVIYRP
                     ATTGSLLGSLPNPFGSNSFLGIRFTGLSTLGVAVHQITSAQAGTDRRVSSVSPEKAVR
                     VTESLSDGALTDPLGYCQFGTPQSLDGCTVYTKRITNFIASKCNGAGDTTTDRNQVFL
                     AYATYVMIHETGHSVGGLAAKYNAAYGGYHYSPTANSVMSQSAQYSTQANKCTWYIPS
                     VWNQVLDPPAIILK"
     gene            complement(3354..4388)
                     /locus_tag="VH419_07725"
                     /pseudo
     CDS             complement(3354..4388)
                     /locus_tag="VH419_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_263577168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
     gene            5484..6476
                     /locus_tag="VH419_07730"
     CDS             5484..6476
                     /locus_tag="VH419_07730"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY7043540.1"
                     /translation="MVRAVAKLVSADIITYTELGLRGQPTTRVVHPQGVVAVELEQIF
                     EHYKGEHPLIEHFREHPQARVAKLSDFLTQRELRRLGIYNEFFRKIGVEHQIVIGLPA
                     SLPRVGGVAFSRSGSDFSERDRMVLSVLQPHIIQAQRNAESLHLARREATLVLAGLEE
                     IGRGIVILRPDGGVSFATRLAREWLTTYFGHPALRASRLPEPLQRWAKLQEAPASRAD
                     DVLAARKPFVVDAEEARLIVRLIPAHGGSLLLLEQQQTVVKPAALEALGLSRREAEVL
                     VWVTQGKTDAAIGEILSVSPRTVAKHLEHIYERLGVENRSAAAAIAFTTPTVAS"
     gene            complement(6506..>6926)
                     /locus_tag="VH419_07735"
     CDS             complement(6506..>6926)
                     /locus_tag="VH419_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043541.1"
                     /translation="LPDTADVSQNNGQAGLFLFNNSSADLYRITLTGNARQGIGASLN
                     STINLHVYDPARPNNLISITGNGTANVVNNNDGISLFSASMLVSPQNPGGPGQVIVTG
                     HPGWGVNCFGTTNKAAAGLDTTGISGNTLGTVNCGGF"
BASE COUNT         1177 a         2076 c         2334 g         1339 t
ORIGIN      
        1 tgctcagcac ggcgaccgga atcgtgaact tgagcccgca cggccagaga ttcttggaga
       61 tgagcggctc gatgtcgatc tcgactcgaa tcgggggcac gccgctcgac ggaggcgtga
      121 tcgcgaccgt ctgcgggatc gacgtcaccg cgccgatcca gatgtcggag atgcagccca
      181 cgccgccggg gagcgtgcac gtgccttgga cgatgttgcg gtccacgaac tggttaccgt
      241 aggtcgcggt cacctcatag gtacccggag tcaagaaggt ggcgtcgtag atctcggtga
      301 ggtcgcaggt cacggcgaat tccgctcccg gtccgatcgt gacgagatcg tccgggatgc
      361 cgtacatctt ctctcggatg atcgggtcca gctgcagacc gccgtccatt accgtgaacg
      421 tggtgttgac acagtcgggt cggatggtcc gcatgggcga tgcggtgttg ttcttgaact
      481 tcgccgtgac cagcacgggc tcgccttgcg gcttcgatgg cggggcggcg agcgtttccc
      541 cggtgtcaag cgcacagacg ttgtccgcct gattgctgtt ggcccgcagc gggcagttgt
      601 cgcaggcgtc cccgacgcga tccccatcgg tgtcggcctg gtcgggattg ggcgtggtgg
      661 gacagttgtc cacggcgtcc cgtacgccgt ctccgtcctg gtcgtggtcg cagaacacgg
      721 tcaggcgggt cgttccgcgc cccccaacgc acgggatcgc cacatcgtag tcgaccgtgc
      781 cgttgttgag gagcttccag gggaaactga caatctggtt ggtgttgaac gagaactcgg
      841 agaagaactg gacgtagcag gtgtcgcact tcaacggcat cgggggaatc gtgaccgtct
      901 ggacctggtc ctgtctgaag gggctcggtt caggcgtgag aaggctgaac aagtggaacc
      961 cgaacggcac acccccgctg ggcggtcccg ccccgagcgc cgtcgcgtcg atctggatgg
     1021 tcttgccgag cacggtggcc gtgttgttcg cggttgagca cggcaatgtg gagttggatg
     1081 ggcacggtct cgtgtccgtt cgcgtgaacg tctcgaatcc ggtgggctcc ccgcttggga
     1141 attgggtcgg gaacctgacc gtgccgtcca ggaggacctc gaagtagcag cacagaaagc
     1201 ccgcctgcat caggagaccc tgaagaggcg cggggaccag ctggaactgg acaacgttgg
     1261 gctcccacgt gttagtgttc gtgtttaaca ccttggagtt ccacgtgttg tggcccgtgt
     1321 tgaacagcag gttggtgtga atgaactggg tcgtggtcag tgcggtcgcg tcgacgaaga
     1381 cggtgtaccc ggtgatgacg aggtggttcg tcccgcgtcc ctggatgaat ccatcgcagg
     1441 tggtgggaaa gttcgggtcc gggaagaaga cactgtagtc ccatgttccc gtcgcggtga
     1501 cctccagctt acagttcatc gcgttcccgg aaccgatgat catctcatag aggcctgggc
     1561 tcaggtccat gttctggatg atgttgttcg gcagctggcc cggctgtgac gcccccggcg
     1621 tgaaccccgt gagtatccag gcgtccaacg tgagtgccgt cgcatcgagc gccatcggga
     1681 aggtcggggg cgggggctgc gcctcggtcc gcgcgacatt cgtgagaccg gcgagcagca
     1741 ctgcgaagac ggcgagggcg acccgactgc ggtgccgacc caatggccct tcgatcggtc
     1801 ccctcatgct agtcggactc gtccgggccc ggcttcaccg ccagtttgat ctccatgctg
     1861 gctgcgcccg ttccggtccc caggaactct ttgaccgact tgacttggta gcacaactga
     1921 ctcggcttag caggacatgc gaagccaggg ggaggggtga taggggtggc ggtcggatcg
     1981 aagaaggatg ccaccgcccg cgagaggagt ttgttcacga cgtcgatctg cccctgagtg
     2041 tcgtcgaagc aaaacggctg cgttccggtc tggctgaagt cgctgttgaa cggtctcacg
     2101 tcaccgcggt gctcaagggt cgtgacgacg aacatgttgt tgatgaagat cgaggagcag
     2161 aaatcgagct ggtcttcggg cacctcaccg agctcgtagg cgatggtcag gaacccggtg
     2221 agctttttcc ccttcgcctg cgggttgatg aggtcctgaa aggggagggc ctcccccggc
     2281 gggatgttgc tctcggccca cgccgccgcc gggacggcgg ccacgacgat cgtcgccacg
     2341 gccagaacgc tcagtgctct caggattcgc gtcatcacgt gccccttttc agtcgttcgt
     2401 tcgtgctcgg catgcgggtt ggacacagtt gtcacggtta cttgaggatg atggcgggcg
     2461 ggtcgagcac ctggttccag accgacggaa tgtaccaggt gcacttgttg gcctgggtgc
     2521 tgtattgcgc cgactgcgac atgacggagt ttgcagtggg cgagtagtgg tatcccccgt
     2581 aggccgcgtt gtatttggcc gccaggcctc ccaccgagtg acccgtctcg tggatcatga
     2641 cgtaggtggc gtaggccaga aatacctggt tgcgatcggt ggtggtatcg ccggcgccgt
     2701 tgcacttgga cgcgatgaaa ttcgtgatcc tcttggtgta gacggtgcag ccgtccagac
     2761 tctgcggcgt cccgaactgg cagtaaccca gggggtcggt cagagcaccg tcgctgagac
     2821 tttcagtgac tcggaccgcc ttttccggcg agacgctgct gacccgccgg tcggtgccgg
     2881 cctgagccga cgtgatctgg tgcacggcga ccccgagggt ggagagaccc gtgaacctga
     2941 ttccgagaaa actgttgctg ccgaacgggt tcggcaggct cccgagaagg ctgcctgtcg
     3001 tcgccggtcg gtagatcacg aagaggtcct tcttgttcgg gtcgacacac tccgccccgg
     3061 catttgggct cccgcacggc tcgaggcgcg tccgggtggt gccgaggtca aggttgagtc
     3121 cagcgcattc ctgtgcatcg gtgaagccgt caccgtcggt atccgctgtc gatcccgggg
     3181 cgatgttggt gcaatcgacg acggcccacg ctggctccgg cgacaccgtc acggcaattg
     3241 ctgccagcag gacgaccagt ccgcccaggg ccccatacag ccacccgggt ccgcgctgtc
     3301 tgttggtcat gaccttctcc cgttggaaac gcctctcgat cgcatcggcc ctattagtca
     3361 tcgggatcct tatcgaagcg cttgagcagc aagacgtccg agtccgtgaa gttgatcgtc
     3421 tgcgtgccga ccttggcctt gcccttcagg atcggattca cgacgaccga cttggacccg
     3481 gcgggaatgt cgccacagcc gaatccggtt tgctggaacc agaaggtgaa gatcatgtct
     3541 ttcaggccgt cgccgttcac atcgaacagg cgccctgacg cgccgatgag gttccgcgtc
     3601 gggtcgagcg cttcggtccc ggttttcccg aacgtcaccg tcttgggatc gatcttgctg
     3661 gcgtcgaact ccgggctgct caacaccgcg accgggatcg tgaacttgag cccgcaggcc
     3721 cagacatttc gggagatgaa cggctcgatg tcgatctcca ctcgggtcgc gggcacgccg
     3781 cccggcggcg gggtgaccgc gacggtctgg gacggcgacg agatcgcgcc gatccagata
     3841 tcggagatgc aacccactcc accgggcagg gtgcacactc cgttgacgat gttgcgatcg
     3901 acgaactggt tgccgtacgt cgcggtgacc tggtaggtac ccgcgctcaa gatcgaggca
     3961 tcgaacgact cggcgaggtc gcaggtgacc gagaattcgg ttccgtccgg gatcgtgacc
     4021 agatcatccg ggatcccgta catccgctcc ttgatgatcg gatcgagcaa gaggttctga
     4081 tcgtccatga cggtgaaggt ggtgttgatg cagtccggtc gaatggtgag gaggggcgcg
     4141 ccgctcgtgt tctggaacct cgccgtgacc atgatcgggt tcccggccgg cgtcggaccc
     4201 gggacgacga cctgctcacc ggtctccccg gcgcaaggat tgtccacgag tggattcgcg
     4261 ctggccgtga gagggcaccg gtcgcacacg tcaccgacgc catcgccgtc ggtatcggcc
     4321 tggtccggat tcggggtgag cgggcagttg tcgacgctgt cgggcacgcc atcgccatcg
     4381 aaatctcgga taccggtgag gccggcgatc cggactcccg tgttgaacag cagggttttc
     4441 accccggtcg ccggatcgat cgtgaacagg tcgccgttgg gcgagccgcc gtagagcgtg
     4501 ccattggaat tgaaccagat gtcggcgatg acgttggtcg cactgccgat cgccgtctcg
     4561 ctcccggtcg taggatcgat cgtcacgagg tcctcggtgt gcccgaccgc gcccccgcgc
     4621 gagccataca ggatgccggc ggggctgaat gcgagacccg gggtgaacgc gttaccgctg
     4681 cccgtctggg tcaccacggt caggatcgcg gcggtggccg gatcgagcgt gatcaaccgg
     4741 ccccgcccat tcaagggtgg atcggccaca atcgtgctgt cccaccctcc accgaagagc
     4801 gtcccgctcg cattgaacct gattgcgtcg acaccctgaa tacagggtgt gatgtcgtcg
     4861 atgcagactc ccgacaaagg atcgcgagcc ggtatggtgc cgacgagggt cgcgacgccg
     4921 ttggtcgtgt tgatcgtgta gagcctcgat ggcccgacgg agccaccgtc cacggcgtag
     4981 agcacgccgc tcgcattgaa gtcgatggcg ccgagcctgt cgagcagcgt gttgccgatg
     5041 agcgtgacga cctgggtgtc ggtgtcgatc cggtagatcc gaccgccgcc gttgctattg
     5101 gagtcaccgg tcgacgcgta cagcgtcaac gccgcgctcg gggacggatg agcaccgaga
     5161 atgaagccag ccaggagagt gaaggccaag aaccttcggt ccatagaaac tctccgtggg
     5221 tcaaccgccg ggcattgcac gccgcgcgga gcgcacacgc gctccgaagg tggcgttgac
     5281 atttatcttg gcgtgggtta gacgtccata cgtacttttg cgtatttcac ttttccggag
     5341 tttacgttgg cagcggtgcg gcacgttctc gccagcgaca cggagcaggg ttgaagcgta
     5401 taggccagcg agacactcag gcgctcgtcg aatgcgtcgc cgggatctat gcgatccggg
     5461 atctcgacac gtttcgcgac gacgtggtcc gcgcggtcgc gaaactcgtt tcggccgaca
     5521 tcatcacata caccgaactc ggccttcgcg ggcagccaac cactcgagtg gtgcatcccc
     5581 aaggcgtcgt cgcggtcgag ctcgagcaga tcttcgagca ttacaagggc gaacatcctc
     5641 tgatcgagca cttcagggag catccgcaag ctcgggtcgc caagctctct gatttcttga
     5701 cccagcgcga gctccgtcgc ctggggatct acaacgagtt ctttcgcaag atcggagtgg
     5761 aacatcagat cgtcatcggg ctgccggctt cgttgccacg agtcggcggc gtcgcgttca
     5821 gccggagtgg ctccgacttc tcggagcgtg atcgaatggt cctgagcgtg ctccagcccc
     5881 acatcattca ggctcagcgg aacgcggagt cgctccacct ggccaggcgc gaggcgacgc
     5941 tggtgctggc ggggctggag gagatcggac gggggatcgt gatattgcga ccggacggcg
     6001 gcgtcagctt cgcgacgcgg ctggcccgcg agtggttgac gacgtacttc ggccacccgg
     6061 cgctgcgggc gagccgacta ccggaaccgc ttcagcgctg ggcgaaactc caggaggcgc
     6121 cggcaagccg tgccgatgac gtcttggctg cccgcaagcc gttcgtggtc gacgccgaag
     6181 aggcccggct catcgtgcgg ctcattccgg cccatggtgg cagtctcctc ttgctggaac
     6241 agcaacagac ggtcgtcaag ccagcggccc tcgaggccct cgggctcagc cgccgagaag
     6301 cagaggtact cgtgtgggtc acgcaaggca agaccgacgc tgccatcggg gaaattctca
     6361 gcgtgagccc ccgcacggtc gccaagcacc tcgagcatat ctacgagagg ctgggcgtcg
     6421 agaaccgatc ggcggcggca gcgatcgcgt tcacgacgcc caccgtggcg tcctgaccta
     6481 tgccctcgtc gttccggctc gctcgctaga acccgccgca gttgaccgtg cccagggtgt
     6541 tgccggagat tccggtcgtg tccagtcccg ctgcggcctt gttcgtggtg ccaaagcaat
     6601 tgacgcccca gccgggatgc ccggtgacga tgacctgccc gggcccgccg ggattctgcg
     6661 gagacacgag catcgaggcc gagaacagcg agatgccgtc gttgttgttg acgacgttcg
     6721 cggtgccgtt gccggtgatg ctgatgagat tattcgggcg ggcggggtcg tacacgtgca
     6781 ggttgatcgt cgagttcagg ctggcgccga tgccttgacg ggcgttgccg gtgagcgtga
     6841 tgcggtagag gtcggccgac gagttgttga agaggaacag cccggcctgg ccgttatttt
     6901 gtgatacatc cgcagtgtcc ggcaag
//
LOCUS       DATBFZ010000250         9841 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_2789766, whole genome shotgun sequence.
ACCESSION   DATBFZ010000250 DATBFZ010000000
VERSION     DATBFZ010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 9841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9841
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2789766"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="VH419_07740"
     CDS             complement(<1..202)
                     /locus_tag="VH419_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007079532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043542.1"
                     /translation="MSTAPTDIRYDQAAIEADETVPMIHIWRNFAGTPEQLFRAHTER
                     DLYQRWVGPAGQTVVIDRWDASE"
     gene            complement(199..561)
                     /locus_tag="VH419_07745"
     CDS             complement(199..561)
                     /locus_tag="VH419_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009075328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY7043543.1"
                     /translation="MSDQLSRVFAALADPTRRDLVARLTDGDATVGQLAAPYAVSLQA
                     VSKHLKVLEEAGLVTRSRDAQRRPVHLEAEVFDLMTKWIERYRQRAEERFGRLDAVLA
                     EMNDEQTETENPMEGTAS"
     gene            complement(910..1323)
                     /locus_tag="VH419_07750"
     CDS             complement(910..1323)
                     /locus_tag="VH419_07750"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEY7043544.1"
                     /translation="MPVVDASVVVDWVAPDADPKGPAMRALLDLSQGEGRLLAPRLMP
                     EEVSNALLTGVRRGRWDGEAADRAFAHLRDLPVDLVDTDADLDQAWELSRRYDEHPLY
                     DLLYVAVARRLGEVLLTADRRLAGRLRDPDVRLVG"
     gene            complement(1310..1609)
                     /locus_tag="VH419_07755"
     CDS             complement(1310..1609)
                     /locus_tag="VH419_07755"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY7043545.1"
                     /translation="MIVVDGVEMVDVREAAKLARRTPETIRRWVWSGRLAATKQGNRL
                     LVPKEAVVARGGQVAAGGREARPSLGDWADRVRAGRTGKRGATAADLVVGDRAGR"
     gene            1709..3232
                     /locus_tag="VH419_07760"
     CDS             1709..3232
                     /locus_tag="VH419_07760"
                     /inference="COORDINATES: protein motif:HMM:NF016330.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HEY7043546.1"
                     /translation="MTLDGWLREADAGSRRRGEAYAAEGRVRVVRRAPDQVVAVVEGT
                     ESYDVQVSTDDAFCTCPIGQRGEVCKHVVATVLAADSGPSGGDPSRSPVVEDIPPQRS
                     RTDRRHLTREQMRELVACLSTRGYLDYDRALDHGRLAHEVVDDLERGLDERSADVMRP
                     LLERAIGTMIRTILRSDDSSGSQGDATWRLLQLHVQAARLGSPDPLRLARWMTKVGFG
                     DDGFFEVDPVPYADALGDRGLAAYRREIDRRLADDPDDFHARRARQRLAVLTGDVGTI
                     VLEVGGPLDRPNHYLDLVDALLEVGAEDEALGFAREGAALPNVPHQTGKLYDVAVRLL
                     TERGDHADAVALRMQQVRGHPVLSTYTTLRRTAGDSADWPTLRLEALDILLRADPEDW
                     LRALLADGDAELAWEASTTMQVSASLLQQLVRSRARTHPADVFDGYVELIDATLVEAR
                     PENYREAVKLLGELRRACAAGGRVDEYDAYVTALLDRHVRRPTLVRMLRAMPPGSAG"
     gene            complement(3242..4009)
                     /locus_tag="VH419_07765"
     CDS             complement(3242..4009)
                     /locus_tag="VH419_07765"
                     /inference="COORDINATES: protein motif:HMM:NF019191.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ltp family lipoprotein"
                     /protein_id="HEY7043547.1"
                     /translation="MSTPAGWYPDPQAEGQQRYWDGNAWTEHQAPFAQPQQPTQSTQP
                     AWSAPHQSAPYAAAAPLAAKQKKPFWRRTWVLVTAGVLVAFIGIGAASGSSDPKDTAA
                     KDNHTADVQKDKTSDAATEAPSTPDVTEAADPEPTPTKTPEPTKTPKPKPSYTASQEQ
                     AIGAAKDYLDYSAFSRTGLINQLSSKYGDGFSKADAIFAVNHIQVDWNEQAVQSAKDY
                     LSMSHFSRVGLIQQLSSKYGDGYTVAQATYAANHVGL"
     gene            4198..7266
                     /locus_tag="VH419_07770"
     CDS             4198..7266
                     /locus_tag="VH419_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020392331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3427 domain-containing protein"
                     /protein_id="HEY7043548.1"
                     /translation="MQEGLYESVLTTGLTSQLKTLTGLEPDIAKVDPADQTHVLTRHL
                     AAAIHARLSVERHPEDRLALANTLLRSIEDRGDSVEHPVRELHGLRRPPEPGRVLRLT
                     QRPRTPLNDAALLTNAHGEPSLANELRAEIDSADAVDLLCAFVMWRGVRLLEGPLARA
                     RRAGVPLRVVTTTYIGGTEREALDRLVRDFGAEVKIQFDAARTRLHAKAWLFRRNTGF
                     DTAYVGSSNLSTSALLDGVEWNVRLSKSATPALLTKFEATFDSYWNSADFEPYDPERD
                     RDRLDDALALARSGGRRDRLTISLSGLEVRAYPFQQEMLDALDAERLLHDRHRNLIVA
                     ATGTGKTVVAALDYRRLCATGERPSLLVVAHRREILEQSLRTYREVLGDGDFGELYLG
                     GARPERWQHVFASVQSLTSYGVSNIPRDAFAIVVIDEFHHAEARTYRTLIEHLQPDEL
                     LGLTATPERADGLDVRSFFEGRTAAELRLWDALGADLLCPFHYFAVADDTDLRSVSWS
                     RGRYDEASLSNVYTGNDARARLIINALRDKVVDIGRMRGLGFCVSVAHAEYMATVFND
                     AGIPARAVSGTTPREERARALSDLRARTVPILFAADLFNEGLDLPEIDTVLFLRPTES
                     ATIFMQQLGRGLRRTPTKAVLTVLDFVGYHRKEFRFDKKLRALTGQTRAGVERGVKEG
                     FPFLPSGSQIVMDRQSQQIILENLRSQIANRWQQVVAELRSYGDTELPTFLQESGVEL
                     SDILRRGTHSWTRLRRDAGLPTLPGSDREDKLLRRVRAFAHVDDPIRVAGYARLLADN
                     APDFEALSSGEQRIARMLYFSLWSDGGGFTSPADGLRALADEPATRREIKSVVDISFE
                     AARHVSLELSGPLSNIPLRLHARYQREEVLAALDFPRMPNSMREGVWYSPELNVDCFL
                     VTLKKSEADYSPTTMYADYPISPTLFHWESQSTTSVESPTGRRYLAGSSNVLLFVRHE
                     QKDEFGTSPYLFLGPVRYVSHTGDRPIAITWRLETPMPIDFFNVGSAVAQ"
     gene            complement(7477..8088)
                     /locus_tag="VH419_07775"
     CDS             complement(7477..8088)
                     /locus_tag="VH419_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017935915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043549.1"
                     /translation="MTTNPRVLLLPGVALGLVTGVLGVQLAHGGGSFEPLHPGDPCAV
                     RDVTSQATGIDGLTERLVLLGLDSAACRLHTSREALTLALAQTDNPIDAQIDALRAGL
                     FDAVGKMEAAGTLPPASALVDDALDATDLSPLVEAAIRALPDSVIDAVLKTDDVLTRT
                     INDLDLRALLAEADDQQSLDQNLDAAVTQAVKDSLIARVQDLL"
     gene            complement(8085..9428)
                     /locus_tag="VH419_07780"
     CDS             complement(8085..9428)
                     /locus_tag="VH419_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017935914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY7043550.1"
                     /translation="MRRAVAPLLTLTVALVLADSAVVTLALPDILVNLQTTVGQVAWV
                     LIAFNLVLGVVAVPTAVVFGLAQPRLLTAAGIAVFAGSSAWCAWAGSIEVLISARCLQ
                     ALGGAFALIGCLELLVAEHGERRGVATWITTGVVGTAMGPVVGGLLTQAFSWQAIFVV
                     QVPCAVLAVPAAFAVHAVHVPTPDSHRLALRPNLALALLSAALTAALFLLVLLLVEGW
                     RRSPAVAALTVSVVPIAALAARPLAHWLRPPSEVEVASGCFLIAGGLVGLAILPSADL
                     AWTVAPQALVGLGLGFTMDRLTSSAMQLRLPRVRHAAWTIGARHLGVVLGLAILTPVF
                     TANLMSAQVPAQEAITSLVLDAPLQPEDKVAVARSLGDELTSQRGRIPDLERAFTTVD
                     VEPAARPAIDRLERDLNGQLERAATHAFRDSFLLGAGLALLALLVVVLPQRRTTS"
     gene            complement(9500..>9841)
                     /locus_tag="VH419_07785"
     CDS             complement(9500..>9841)
                     /locus_tag="VH419_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043551.1"
                     /translation="GEDHRHGSRLPPLHDLRYQDAPRRLLLRLRRLRQHQRLQLIVAR
                     TSDAIGSQWVDWFPIAAFGSPLRSGAWGRLDVLRVTDALYVEQTVQLGLRVLSGDARL
                     AKACEVVTLLR"
BASE COUNT         1587 a         3369 c         3297 g         1588 t
ORIGIN      
        1 cctcgctggc gtcccagcgg tcgatcacga cggtctggcc ggccgggccg acccatcgct
       61 ggtagaggtc gcgctcggtg tgggcacgga acagctgctc cggcgtaccg gcgaagttgc
      121 gccagatgtg gatcatcggc acggtctcgt cggcctcgat ggcggcctgg tcgtagcgga
      181 tgtcggtggg tgcggtgctc atgatgcggt tccctccatc gggttctcgg tctcggtctg
      241 ctcgtcgttc atctcggcca ggacggcgtc gaggcgcccg aagcgctcct cggcccgttg
      301 gcggtagcgc tcgatccact tggtcatcag gtcgaacacc tcggcttcca ggtgcacggg
      361 gcggcgctgg gcgtcgcggc tgcgggtcac cagcccggcc tcctcgagca ccttgagatg
      421 cttggacacg gcctgcaggc tcacggcgta cggcgcggcc agctggccga ccgtggcgtc
      481 accgtcggtc agccgagcca ccaggtcacg gcgggtcggg tcggcgaggg ccgcgaacac
      541 ccgcgacagc tggtcggaca tctcgtgcct ttctgcaacc attaggttga gaacgacggt
      601 aggcgtgtga cgaccgtttg tcaacccgtc ggttgaagaa ccctccccgc cgtgcggaga
      661 acacgctgga gccgcgcacc ggtcgaccgc ctgctctccc gccgccgccc ggcaccccag
      721 tcgctcgcca gcaccaacga gatggggcgg ccacaccgtc accgtcctcc cgggcggcgt
      781 gaccctcatc cggatgcgca acagccggca agacgacgac ccgaccaacc cgcaggtcgc
      841 gatcaacgct ctcgccgacc agctgtctcc cctgcgcggc ggcgcgccga cctgaccggc
      901 gggcctccgt cagccgacca ggcggacgtc ggggtcacga agtcggccgg ccagcctccg
      961 gtctgcggtc aacagcacct cgcccagccg ccgggccacc gcgacgtaca gcaggtcgta
     1021 gagcggatgc tcgtcgtagc gacgcgacag ctcccaggcc tggtcgagat cggcatcggt
     1081 gtccaccagg tcgaccggaa ggtcgcgcag atgggcgaat gcccgatcgg cggcctctcc
     1141 gtcccaccgc ccccgtcgta cgccggtgag cagcgcgttc gacacctcct ccggcatcaa
     1201 ccgaggtgcg aggagccgcc cttccccttg ggaaagatcg agtagggctc gcatcgccgg
     1261 gcccttcggg tcggcatccg gcgcgaccca gtcgacgacg acgctggcgt caacgaccgg
     1321 cacggtcgcc caccaccagg tcggccgctg tcgctccgcg cttcccggtg cggccggccc
     1381 tgacccggtc ggcccagtcc cccagggacg ggcgcgcctc gcggcccccc gcggccacct
     1441 gaccgccccg agcgaccaca gcctccttcg gaaccaagag ccgattgccc tgcttggtgg
     1501 cggcgagacg gccgctccac acccaccggc ggatggtctc cggcgtacga cgcgcgagct
     1561 tggcagcttc gcgcacgtcc accatctcta caccatccac gacgatcatg tagtaactct
     1621 actacgccac cgatcaatgt cgcccccgtc cgccactcgt ggaccaccga cgcatttcga
     1681 gtgactcggg gtgtggtcgg atggcgacgt gaccctcgac ggctggcttc gagaagccga
     1741 tgcaggctct cgtcgccgcg gcgaggcgta cgccgcggag ggacgggttc gagtcgtgcg
     1801 acgcgcgcca gaccaggtgg ttgcggtcgt ggaaggcacc gagtcgtacg acgtccaggt
     1861 ctcgaccgac gatgcgttct gcacctgccc gatcggccag cgcggcgagg tctgcaagca
     1921 cgtcgtggcc accgtgcttg cggcggactc cggcccctcc ggaggagacc cgtcacgaag
     1981 tcccgtggtc gaggacatcc ctccgcaacg gtcgcgaacc gaccgccgcc acctgactcg
     2041 cgaacagatg cgcgagctgg tggcttgcct ctcgacccgg ggctatctcg actacgatcg
     2101 cgccctcgat cacggtcggc tcgcacacga ggtcgtcgac gatcttgagc gcgggctgga
     2161 cgagaggtcc gccgacgtca tgcggccgct gctcgagcgc gcgatcggca ccatgatccg
     2221 taccatcctg cgttccgacg actcgtcggg ctcgcagggt gacgcaacgt ggcggctgct
     2281 ccagcttcac gtccaggctg cccgtctggg ctcacccgac ccactccgcc tcgctcgctg
     2341 gatgaccaag gtcgggttcg gcgacgacgg cttcttcgag gtcgacccgg tcccgtacgc
     2401 cgatgcgctg ggcgaccgcg ggctggcggc gtaccgccgc gagatcgacc ggcggctggc
     2461 cgacgacccg gacgatttcc acgcgcggcg ggcacgccag cgactcgcgg ttctcacagg
     2521 cgatgttggc acgatcgtcc tcgaggtcgg tggcccgctg gaccgtccga accactacct
     2581 ggacctcgtc gacgcccttc tggaggtcgg tgccgaagac gaggcgctgg gcttcgcgcg
     2641 cgagggcgcg gcgctgccga acgtgccgca ccagaccggc aagctgtacg acgtcgcggt
     2701 gcggttgctg accgagcggg gcgaccacgc cgacgccgtc gccctgcgga tgcagcaggt
     2761 gcgaggccac cccgtgctgt cgacgtacac caccctgcgc cgcacggccg gcgactccgc
     2821 cgactggccg acgctgcggt tggaggccct cgacatcctg ctccgcgccg accccgagga
     2881 ctggctgcgc gcgctcctcg ccgacggcga tgccgaactg gcctgggaag cctcgacgac
     2941 gatgcaggtc agcgcatccc ttttgcagca gctggtcagg tcacgagccc ggacccaccc
     3001 ggccgacgtc ttcgacggct acgtcgagct gatcgacgcc accctggtcg aggccaggcc
     3061 cgagaactac cgcgaggccg tcaagctctt gggcgagttg cgccgcgcgt gcgcggccgg
     3121 cggccgggtc gacgagtacg acgcgtacgt caccgccctg ctcgaccgcc acgtccgccg
     3181 acccaccctg gtccggatgc tccgcgcgat gccgcccggc agcgccgggt gaggcgaccg
     3241 cctagagccc gacgtggttc gcggcgtacg tcgcctgggc cacggtgtag ccgtcgccat
     3301 acttcgacga cagctgctgg atcagtccaa cgcgcgagaa gtgcgacatc gacaggtagt
     3361 ccttggccga ctggaccgcc tgctcgttcc agtcgacctg gatgtggttg accgcgaaga
     3421 tggcgtctgc cttgctgaac ccgtcgccgt acttcgacga cagctggttg atgaggccgg
     3481 tccgcgagaa tgcggagtag tccagatagt ccttggccgc gccgattgcc tgctcctggc
     3541 tcgcggtgta cgagggcttg ggcttgggtg tcttcgtcgg ctccggagtc ttggtgggag
     3601 tcggctccgg gtcggccgcc tccgtgacgt ccggggtgga cggcgcctcg gtggccgcgt
     3661 ccgacgtctt gtccttctgc acgtcggcgg tgtggttgtc cttggcagcc gtgtccttcg
     3721 ggtcgctgct gccgctcgcc gcaccgatgc cgatgaaggc caccaggaca ccagcggtga
     3781 ccagcaccca cgtacgacgc cagaacggct tcttctgctt cgctgccaac ggcgcagcgg
     3841 cggcgtacgg cgcgctctgg tgcggcgcgg accaggcagg ctgagtggac tgggtgggct
     3901 gctggggctg ggcgaacgga gcctggtgct ccgtccaggc gttgccgtcc cagtagcgct
     3961 gctggccctc ggcttggggg tccgggtacc aaccggcggg tgtgctcatg cgaaagatct
     4021 cctcgagatt gcgtgtgatg cggccggacc gtaacccgca gctcgagccg tcctaaggcg
     4081 aatttgcaaa ggcaaccgat cgatcactcg actagtcccg gaggacccca cgagccccga
     4141 cgggccgatg ttctaaggcc accatcccga ctgtcgacca atggcaagct gcggcctatg
     4201 caggaagggc tctatgaatc ggttctcacc accgggttga cgtcgcaact caagacgttg
     4261 accggcctcg agccagatat agcgaaggtc gatcccgctg atcagacgca cgtgcttacg
     4321 cgccacttgg ccgccgcgat ccatgcgagg ctctccgtcg agcgacaccc ggaggacagg
     4381 ttggcactcg ccaacactct cctccgctcc atcgaggatc gtggcgattc ggtggagcac
     4441 ccggtccgtg agctacacgg tcttcggagg ccccctgaac cggggcgcgt cttgcggttg
     4501 actcagcgtc cgagaacccc gttgaacgac gctgccctcc tcacgaacgc ccacggcgag
     4561 ccgagcctgg caaacgagct cagggccgag atcgactcag ccgacgccgt agatctccta
     4621 tgcgcctttg tcatgtggcg cggtgtgcgg cttctggaag gtccgttggc gagggcgcga
     4681 agagccggcg tacctctccg tgtcgtgacc acgacgtaca tcggcggaac tgagcgggaa
     4741 gccctcgatc gactagttcg cgacttcggt gcggaggtca agatccagtt cgacgccgcc
     4801 cgcaccaggc tgcatgcaaa ggcgtggctc ttccgccgga acactggctt cgacacggcg
     4861 tacgtcggct cctcgaactt gtccacgagt gctctcctgg atggggtcga gtggaatgtt
     4921 cgactgtcaa agagcgcgac gcccgcgttg ctcacgaagt tcgaggcgac tttcgactcg
     4981 tattggaaca gcgccgactt cgagccgtac gaccctgaac gcgatcgtga tcggctcgac
     5041 gacgccctgg cccttgcccg ctctggcggg aggcgcgatc gtctgaccat ctccctctcg
     5101 gggctcgagg tgcgtgccta tcccttccag caggagatgc tagacgccct agacgcggag
     5161 cgacttctgc acgatcgcca ccgcaacctc atcgtcgcag ccacgggcac cggcaagacg
     5221 gtggtcgccg cgttggatta tcgacgattg tgcgcaaccg gtgaacggcc ctctctgctt
     5281 gtcgtcgcgc atcgccggga gatcctcgag cagtccctgc ggacctaccg ggaggtcctg
     5341 ggcgacggcg acttcggtga gctgtacctg ggtggtgccc ggcccgagcg ctggcagcac
     5401 gtgttcgcca gcgtgcaatc gctgacgtcg tacggcgttt ccaacatccc acgtgatgcg
     5461 ttcgcgatcg tggtcatcga tgagttccac cacgcagagg cgcgcacgta ccgaacgttg
     5521 atcgaacacc tgcaacctga cgaactcctc ggcctcactg cgacaccgga acgtgctgat
     5581 ggtttggacg tccggtcctt cttcgagggg cggaccgcgg ccgagctgcg cttgtgggat
     5641 gcgctcggcg ccgatctgct ctgcccgttc cactattttg ccgtcgctga cgacaccgac
     5701 cttcggtccg tgagttggtc acgcggccgc tacgacgagg cctcgctctc caacgtctac
     5761 acggggaacg acgcacgggc ccgcctcatc atcaatgcgc tacgcgacaa agtcgtcgac
     5821 attggcagga tgcgcggcct cggtttctgc gtcagcgttg cgcacgccga gtacatggca
     5881 accgtcttca acgacgccgg gattccggca cgagccgtca gcggcacgac acctcgtgag
     5941 gagcgcgctc gcgcactgtc ggaccttcgc gctcgaactg tgcccatcct gtttgccgcc
     6001 gatctcttca atgagggtct cgatcttcct gagatcgaca ccgtcttgtt cctccgaccc
     6061 acggagagcg ccacgatctt catgcagcaa ctgggccgag gacttcgccg cacaccgacc
     6121 aaggcggtgc tcaccgtgct cgactttgtc ggctatcacc gaaaggagtt ccggttcgac
     6181 aagaagctcc gagcgctgac gggtcagacc agggctggtg tcgagcgtgg cgtgaaggag
     6241 ggcttcccct tcttgccctc gggttcccag attgtgatgg atcggcagtc ccaacagatc
     6301 atcctcgaga acctgcgatc tcagatcgcg aatcgttggc agcaggtggt ggccgagctt
     6361 cggtcttacg gggacaccga gcttccgacg ttccttcaag agtctggcgt cgagttgagc
     6421 gacattcttc gtcgcggcac ccactcgtgg acgcgcttgc ggcgcgacgc cggactgccg
     6481 acgctcccgg gctctgaccg cgaagacaag ctgctaaggc gagtccgggc gttcgcgcac
     6541 gtcgatgatc ccattcgggt tgcaggctat gcgcgtctcc ttgccgacaa cgccccggac
     6601 ttcgaggcgt tgtcgtcggg tgagcagcgg atcgcgcgga tgctgtactt ctcgctgtgg
     6661 tccgacgggg gcggattcac atcaccagcg gatgggttga gagcgctcgc agacgagccc
     6721 gcaacgaggc gtgagatcaa gtcggtggtc gacatctcgt tcgaggctgc tcgccacgtc
     6781 tctctggaac tcagcggtcc actctcgaac atccccttga gacttcacgc ccgctaccag
     6841 cgcgaggagg tgctcgccgc attggacttt ccccgcatgc ccaactcgat gcgcgagggt
     6901 gtgtggtact cacccgaact caacgttgat tgcttccttg tcacgctcaa gaagtcggaa
     6961 gcggactact cgccgaccac gatgtacgcc gactacccca tctcacccac gttgttccat
     7021 tgggagtcgc agtccaccac gtccgtcgag tccccgacgg gtcgtcggta tctcgccggc
     7081 tcgagcaacg tcttgttgtt cgttcgacac gagcagaagg acgagttcgg cacctcgccc
     7141 tacctgttcc tcgggccggt tcgatacgtc tcccacacgg gcgatcggcc gatagccatc
     7201 acatggcgct tggagactcc gatgcccatc gacttcttca acgttggctc cgcggtagcg
     7261 cagtaactcc gctcgcggtg ccgctgatcc gccagttcgt aggctcgtcc cgtcgccgac
     7321 agcggcacat ggcaacggct acgccgcgtg aaggctgcct aggacccgca cgtcctgcgc
     7381 cgcggcaacc atcaagtagc gtccgcgtga agcccgcagt gcttctggtc cgaaatcacg
     7441 agacgggtcg gcctcagggt gcgcagggtc ctcggatcag aggaggtcct gcacccgtgc
     7501 gatcaacgag tccttcacgg cctgggtgac ggcggcgtcc agattctggt cgagactctg
     7561 ttggtcatcg gcctcggcga gcagggcgcg gaggtccagg tcgttgatcg tgcgggtgag
     7621 cacatcatcg gtcttcagga ccgcatcgat caccgagtcc gggagggcgc ggatggcggc
     7681 ctcaacgagg ggactgaggt cggtggcgtc gagggcgtcg tctacgagag cggaggctgg
     7741 tgggagcgtg cccgcggcct ccatcttccc gacggcgtcg aaaaggccgg cgcggagggc
     7801 gtcgatctgg gcgtcgatgg ggttgtcggt ctgcgcgagc gccagtgtga gggcctcgcg
     7861 gctggtgtgg agccggcacg ctgcgctgtc cagcccgagg aggacgaggc gttcggtgag
     7921 accgtcgatg ccggtggcct gggaggtgac gtcgcggacg gcgcaggggt cgccggggtg
     7981 gagtggctcg aaggagccgc cgccgtgggc tagctgtacg ccgagcactc cggtcaccag
     8041 acccagcgcg acgccgggca ggaggaggac gcgtgggttg gtcgtcatga ggtcgtccgt
     8101 cgttgtggga ggacgaccac gagcagtgcg aggagggcga gcccggcacc gaggaggaac
     8161 gagtcgcgga acgcgtgagt cgcggcgcgc tccagctgcc cgttcagatc acgttcgagc
     8221 cggtcgatgg ccggacgggc cgcgggttcg acgtccactg tcgtgaaggc gcgctcgaga
     8281 tcggggatgc gacctcgttg ggaggtcagc tcgtcgccga gcgagcgggc gaccgcgacc
     8341 ttgtcctccg gctgcagtgg ggcgtcgagc accaaggacg tgatcgcctc ctgggcaggc
     8401 acctgcgcgc tcatcaggtt cgcggtgaag accggcgtca ggatcgccag gcccaaaacg
     8461 acaccgaggt gacgggcgcc gatcgtccac gcggcatgcc gcacccgcgg caggcgcagc
     8521 tgcatcgctg aagaagtgag ccggtccatg gtgaatccca ggccgagccc caccagcgcc
     8581 tgcggtgcga ccgtccacgc gaggtccgcg gacggcagga tcgcgagacc gaccagcccg
     8641 ccggcgatca agaagcagcc cgaggcgacc tcgacctcgc tcggcggacg cagccagtgg
     8701 gcgagcggac gtgcggcgag tgctgcgatc ggtacgacgg acaccgtcaa cgccgcgacg
     8761 gccggcgaac gacgccagcc ctccaccagc agcaggacca ggaggaacag tgccgcagtc
     8821 agggcggccg acagcagggc gagggccaga ttgggacgca gcgccagacg gtggctgtcc
     8881 ggtgtcggca cgtgtacggc gtgaacagcg aacgccgcag gaaccgcgag caccgcgcac
     8941 ggcacctgca ccacgaagat ggcctgccag gagaacgcct gggtgagcag tccgcccacc
     9001 accggtccca tggccgtacc caccacacct gtcgtgatcc aggtcgcgac cccccggcgc
     9061 tcaccgtgct cggcgaccag gagctcgaga cagccgatca gcgcgaacgc gccgccgagt
     9121 gcctgcaggc aacgggccga gatcaggacc tcgatcgacc ctgcccacgc acaccaggcc
     9181 gatgacccgg cgaacaccgc gatcccggcg gcggtgagga ggcgcggctg ggcaaggccg
     9241 aacaccaccg ccgtcgggac ggccacgacc ccgagcacga ggttgaacgc gatcaacacc
     9301 cacgcgacct gcccgacggt cgtctgcagg ttcaccagga tgtcgggcag cgcgagcgtc
     9361 accaccgccg agtcggccag caccagcgcc acggtcagcg tgagcagcgg agccacggcg
     9421 cgacgcaccc ggtcatcctt gcattgcacg aaccgacatc gacgtgggaa gcagccggtc
     9481 caactcggcg atgtcggcac taccgcagca gggtgaccac ctcacaggcc ttggccagcc
     9541 gggcatcacc ggacagaacc cgcaggccga gctgaaccgt ctgctcgacg tacagggcat
     9601 cggtgactcg caacacgtcg agtcgacccc aagcgccgga gcggagaggc gacccgaacg
     9661 cggcgatggg gaaccagtcg acccactggc tcccgatcgc gtcggacgtg cgcgcgacga
     9721 tcagctgcag ccgctggtgc tgccgcagcc ttcgcagacg tagcaggagc cggcggggcg
     9781 catcttggta ccgcaggtca tgcagagggg ggagtcgact gccgtgccgg tgatcttctc
     9841 c
//
LOCUS       DATBFZ010000251         1501 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_3447957, whole genome shotgun sequence.
ACCESSION   DATBFZ010000251 DATBFZ010000000
VERSION     DATBFZ010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3447957"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            263..1354
                     /locus_tag="VH419_07790"
     CDS             263..1354
                     /locus_tag="VH419_07790"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEY7043552.1"
                     /translation="MFESTTVSVGLDVHARSIRLAAVRADELLEECTLVYDEEAVERV
                     LRRWPAVRCCYEAGPTGFGLYRSLVDRGIDCWVVAPGLVPQRPGDRVKTDSRDARKLA
                     RLLAGGLLEPIHVPSREFEAARDLVRAREDARLDRMRDRHRLSKFCLRHGRRLPTSSW
                     TVVRRKWLGEQRFEFAAEQCTFDTYVHTVDLVDARIEQLERAIRETAEQGPWSQLVAR
                     LRCLRGVDTLTALALAAEIGDFDRFESAEEFMAFVGLVPTERSSGEQRRQGSITKVGN
                     SHVRRLLVESAWHARRHPKVGYDLARRQRGHHPVVIERSWRCQQRLYQRWQRMAGRGK
                     PQQKIVVACARELAGFVWAIATDQPLRTA"
BASE COUNT          232 a          483 c          527 g          259 t
ORIGIN      
        1 cagaaaacga tgggagatcg acatgtctga ctctcgcacc gcgtccggcg cactcgacca
       61 cgtcgttgtc gtgttgttcg agaaccggtc gcttgacaac gtgctcggac acctctacgg
      121 ccccgaggac ggtaagacag gatggagccg tggccctggg gctgcggcgt ctggttgtcg
      181 gcggagagtg gtcggcgcct gcgcatatgt cgtgggttgc cggggtttgg cccggtagag
      241 ccacatacgg aggtcgccga ccatgttcga gtctacgacg gtgagtgtcg ggctggacgt
      301 ccatgcgcga tcgattcggc ttgctgcggt gcgtgccgac gagctgctgg aggagtgcac
      361 gctggtctac gacgaggagg cggttgagcg ggtgctgcga cgctggccgg cggtgcgttg
      421 ctgttacgag gcgggcccga ccgggttcgg tctctatcgg tctctcgtcg atcgcgggat
      481 cgactgctgg gtggtcgcgc ccgggctggt gccgcagcgg ccgggcgacc gggtcaagac
      541 cgatagccgt gatgcgcgca agttggcgcg gttactcgct ggtggcctgc tggagccgat
      601 ccatgtgcct tcccgggagt ttgaggccgc acgcgatctc gttcgtgctc gcgaggacgc
      661 tcgcctggat cggatgcgtg accgtcaccg gttgtcgaag ttctgcctcc gccacgggcg
      721 cagattgccg accagttcgt ggacggtcgt gcgcaggaaa tggcttggcg agcagcggtt
      781 cgagttcgct gccgagcagt gcacgttcga cacctatgtc cacaccgtcg acctggtcga
      841 tgcgcggatc gaacaactgg aacgcgcgat ccgtgagacg gccgagcagg gcccgtggtc
      901 gcaactggtg gcccgtctgc gctgtttgcg gggcgtcgac accctgacag cgctcgcgct
      961 ggccgccgag atcggcgact tcgaccggtt cgagtcagct gaggagttca tggcctttgt
     1021 cggactcgta ccgacggagc gctcctccgg agagcagcgc cggcaaggct cgatcaccaa
     1081 ggtcggcaac agccacgtcc ggcggctgct ggtcgagtca gcctggcacg cgcggcgaca
     1141 cccgaaggtc ggctacgacc tcgcccgccg gcaacgcggc catcacccgg tggtgatcga
     1201 acgctcgtgg cgctgccagc agcgcctcta ccaacgctgg caacggatgg ccggccgcgg
     1261 caagccgcag cagaagatcg tcgtcgcctg cgcccgcgag ctcgccggat tcgtctgggc
     1321 gatcgcgacc gaccagccac tcaggaccgc ctaaccccgc caagcctctc ggcctcgagc
     1381 agcggatgcg tccgaccaca cggagaaccc tcctgagttc tttgcggcac cgccgatcgg
     1441 tgacccgcga cctaagaccg aggcagctcc cgacggacac cagtcatgcc gtcccgaccg
     1501 g
//
LOCUS       DATBFZ010000252         4170 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_167315, whole genome shotgun sequence.
ACCESSION   DATBFZ010000252 DATBFZ010000000
VERSION     DATBFZ010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 4170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4170
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167315"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..806
                     /locus_tag="VH419_07795"
     CDS             <1..806
                     /locus_tag="VH419_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012924315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEY7043553.1"
                     /translation="SRLATGMMTPREGAALRDRDGVRLLDAMEVAGMEPNLGKARWLD
                     DVDVFVELHVEQGRDLVHRDEPVGVASASWAHGRWRFDFVGEPNHAGATAMEDRHDPM
                     LTYAMTALGANKQARLSEARATFGRVSVQPNITNAIPSRVQAWLDARGADAELVQRVV
                     ETITRQAMERAERDGTSLDVTTESIVPTVDFDPLLAGRLSSLLGGCPIIPTGAGHDAG
                     ILQLAGIPSAMLFVRNPTGISHSPEEYAETADCLSGVDALTTVVRELVT"
     gene            803..2125
                     /locus_tag="VH419_07800"
     CDS             803..2125
                     /locus_tag="VH419_07800"
                     /EC_number="3.5.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamate deiminase"
                     /protein_id="HEY7043554.1"
                     /translation="MTAYWCEKAWLGPGRVTDGVLVRVEGARISDVTTVGAAPADAIR
                     LAGLTIPGFANCHSHAFHRALRGRTQRERGSFWTWRDTMYGVAERLDPDRYYALAKAT
                     YEEMLLCGITAVGEFHYLHHAPNGLPYDEPNAMGLAVVAAARDAGLRIALLDTCYLSA
                     GFGAAPDGVQKRFSDGDADRWAGRVDALTRHREVQSDDIVVGTAIHSVRAVPRAELTV
                     VAKAQAEAPLHIHLSEQPAENDGCVATYGLTPTQLLAEEDVLGDRLSAVHATHLTPTD
                     RSLLGDAKAFACFCPTTERDLADGVGPAAELVEAGSQLTLGSDSHAVINPFEEMRAVE
                     LNARLATRERGHLRAADLLEAGTVWGHTSLGFAHAGRIAGGQWADLVTVDTRSPRTAG
                     TGEGEETVVFAAGPADVRHVVASGRRVDLDAADVGGRLAQAVTSVVEG"
     gene            2125..3291
                     /gene="hutI"
                     /locus_tag="VH419_07805"
     CDS             2125..3291
                     /gene="hutI"
                     /locus_tag="VH419_07805"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020574834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HEY7043555.1"
                     /translation="MPSVVFDHIGELVTNDPSRGDVPGVLRDAAVVVEGDHIAWVGDA
                     ASAPAADERLDIGGAAMLPGFVDSHAHLVFAGDRAAEFAARMEGRPYSAGGIATTVAA
                     TRQASDEALGTNVARLVGEMARQGTTTVEIKSGYGLTTYDEARCLAVARQFTEETTFL
                     GAHVVPAEYADDPAGYVDLVTGPMLEACAPHARWVDVFCERGAFDADQARAVLDAGTS
                     AGLLPRVHANQLGPGPGIAVACERGAAAADHCTFATSDDIGNLADAGVVATLLPGVEF
                     STRSPYPDARAMLDAGVTVALATDCNPGSCYTTSMPLCIALAVREMRMSPAEAVWAAT
                     AGGAAALRRDDVGRVAVGAAADLTVLAAPSYVHLAYRPGVPLVHSVYARGRRIA"
     gene            complement(3308..>4170)
                     /locus_tag="VH419_07810"
     CDS             complement(3308..>4170)
                     /locus_tag="VH419_07810"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEY7043556.1"
                     /translation="LVATIPLPDPDWLVSAAGDVWVKRDDGQVSRIDANSGRAVASLP
                     TGYSDLPACQGLTSDGTRLWTCSGENRLVTLDPRTNKTGERVRVSRLSDQTRFAVGHD
                     LLWVVKGNATTLSGLNLQNGSIAATVDLGTFCIDLARPANIENDRLYVVCPTDGLVLA
                     VDVASGSVTDRLQLDDPRLAAVADDLWVGFSGGLAQIDTDTMTVTAVYPVTPGLFGSI
                     WVEGQDVWVRVPGSAVLTHIDGRRHRIVEVLTSHAYPSGGDVLVFDGSLWTTAADDGV
                     LLKLDLAPSD"
BASE COUNT          603 a         1424 c         1530 g          613 t
ORIGIN      
        1 gctcgcggtt ggcgaccggg atgatgacgc cgcgcgaggg tgcggcgttg cgggatcgcg
       61 acggagtgcg gctgctggac gcgatggaag tcgccggcat ggagccgaac ctcggcaagg
      121 cgcgctggct tgacgacgtc gatgtgttcg tcgagctgca tgtcgagcag ggccgcgacc
      181 tcgtccaccg tgacgagccc gtcggcgtgg cgtccgcgag ctgggcgcac ggccggtggc
      241 gcttcgactt cgtcggtgag ccgaaccatg ccggggccac ggccatggag gacaggcacg
      301 atccgatgct cacctatgcc atgaccgcgt tgggggccaa caagcaggcg cggctgagtg
      361 aggcgcgggc gacgttcggc cgcgtttcgg tgcagccgaa catcacgaac gccatcccgt
      421 cgcgcgtcca ggcctggctc gacgcccgcg gagcggacgc cgagctcgtc cagcgagtcg
      481 tcgagaccat cacgcgccag gccatggaac gagccgagcg ggacgggacg tcgctcgacg
      541 tcaccacgga gtcgatcgtg ccgacggtcg acttcgaccc gctgctggcc ggccggctgt
      601 cgtcgctgct gggcggttgc ccgatcatcc cgaccggagc cggacacgac gccggcatcc
      661 tccagctggc cggcatcccg tcggcgatgc tgttcgtccg caatccgacc ggaatctcgc
      721 actcgccgga ggagtacgcc gagacggccg actgtctgtc cggcgtggac gcgctgacca
      781 ccgtggtcag ggagctggtc acgtgacggc gtactggtgc gagaaggcgt ggctcgggcc
      841 gggacgcgtg acggacggcg ttctggttcg cgtcgagggt gcacgtatca gcgacgtcac
      901 cacggtcggt gcagctcccg cggacgcgat tcgccttgct ggactgacga ttcccgggtt
      961 cgcgaactgc cacagccacg cgttccaccg ggctcttcgc ggccggaccc agcgcgagcg
     1021 cggctcgttc tggacgtggc gcgacacgat gtacggcgtt gcggaacgac tcgacccgga
     1081 tcgctactac gcgctggcca aggcgacgta cgaggagatg ctgctgtgcg ggatcacggc
     1141 ggtcggtgag ttccactacc tgcaccacgc accgaacggg cttccgtacg acgaacccaa
     1201 cgcgatgggt ctggcggtgg tggcggctgc tcgggacgcg ggtctgcgca ttgccctcct
     1261 ggacacctgc tacctctcgg cgggcttcgg cgccgcgccg gacggcgtgc agaagcggtt
     1321 cagcgacggc gacgcagacc gctgggcagg ccgggtcgat gccctcaccc gacatcgcga
     1381 ggtacagagc gacgacatcg tggtcgggac ggcgatccac tcggtacgtg ccgtgccgcg
     1441 cgccgagctc acggtcgtgg cgaaggcgca ggcggaggct ccgctgcaca tccacctctc
     1501 ggagcagccg gccgagaacg acgggtgcgt cgcgacatac gggctgaccc cgacgcagtt
     1561 gctggcggag gaggacgtgc tcggcgaccg gctgtcggcg gtgcacgcga cccatctgac
     1621 gccgaccgac cgaagtctgc tcggcgacgc gaaggcgttc gcctgcttct gtccgaccac
     1681 cgagcgcgac ctggccgacg gcgtcgggcc ggcggccgag ctcgtcgagg cggggagcca
     1741 gctgacgctg ggctccgaca gccacgccgt catcaacccg ttcgaggaga tgcgagcggt
     1801 cgagctgaac gcccggctcg ccacacgcga gcggggccac ctgcgcgcgg ctgacctgct
     1861 cgaggcgggc accgtgtggg ggcacacgtc gctcggcttc gcccacgccg gccggatcgc
     1921 cggggggcag tgggccgacc tcgtcacggt ggacactcgg tcgccgcgga cagccgggac
     1981 gggggagggc gaggagacgg tcgtcttcgc cgccggtccc gccgacgtcc ggcacgtcgt
     2041 ggcgagcggg cgacgcgtcg acctcgacgc cgcggacgtc ggtggccggc tggcccaggc
     2101 ggtgacctcc gtggtggagg gctgatgccg agcgtcgtgt tcgaccacat cggtgagctc
     2161 gtcacgaacg acccaagccg cggcgacgta cccggcgtac tgcgcgacgc ggccgtcgtc
     2221 gtcgagggag atcacatcgc ctgggtgggt gatgccgcat cggctcccgc tgccgacgaa
     2281 cggctcgaca tcggtggcgc ggcgatgctt ccggggttcg tggacagcca cgcgcacctg
     2341 gtgtttgccg gtgaccgtgc ggcggagttc gcggcccgga tggagggacg cccgtactcc
     2401 gcggggggga tcgcaacgac ggtggcggcg acgcggcagg ccagcgacga agcgctcggc
     2461 accaacgtcg ccaggctggt cggcgagatg gcccgccaag gcacgaccac ggtcgagatc
     2521 aagtccggct acgggctcac gacgtacgac gaggcgcgct gccttgcggt ggcgcgccag
     2581 ttcaccgagg agaccacgtt cctgggcgcc cacgtcgtac cagccgagta cgccgacgac
     2641 ccggccggct atgtcgacct cgtcaccggg ccgatgctcg aggcgtgtgc cccgcacgca
     2701 cgctgggtcg acgtcttctg cgagcgcgga gccttcgacg ccgaccaggc gcgggctgtg
     2761 ctggatgcgg gtacgtcggc cgggctgctg ccccgcgtcc acgccaacca gctcggcccc
     2821 gggccgggga tcgcggtggc gtgtgagcgc ggcgccgcgg cggcggacca ctgcacgttc
     2881 gcgacgtcgg acgacatcgg caaccttgcc gatgccggcg tggtggcgac gctgctgccc
     2941 ggtgtggagt tctccactcg ctcgccctac ccggacgcgc gcgccatgct cgacgccggt
     3001 gtcacggttg cgcttgcgac cgactgcaac ccgggctcgt gctacaccac gtcgatgccg
     3061 ctgtgcatcg cgttggctgt ccgtgagatg cggatgagtc cggccgaggc ggtgtgggca
     3121 gcgaccgccg gtggcgccgc ggcactgcgc cgtgacgatg tcgggcgtgt cgccgtcgga
     3181 gcggccgctg acctcaccgt gctggccgcc ccgtcatacg tccatctcgc ctaccggccc
     3241 ggcgtccccc tcgtgcactc cgtgtacgcg cgtggccgcc gcatcgcctg accgccaccg
     3301 ggcggggtca gtcggacgga gcgaggtcca gcttcaacag cacaccgtcg tcggccgcgg
     3361 tcgtccagag ggagccgtcg aacaccagga cgtcgccacc gctgggatac gcatggctcg
     3421 tgaggacctc gacgatgcgg tggcgtcggc cgtcgatgtg ggtgaggacg gcgctgcccg
     3481 gtacccgcac ccatacgtcc tgcccctcca cccagatcga gccgaagagc ccaggtgtga
     3541 ccggatacac ggcggtcacg gtcatcgtgt cggtgtcgat ctgggcgagc cctccggaga
     3601 acccgaccca caggtcgtcg gcaacggcgg ccagccgtgg gtcgtccagc tgaagccggt
     3661 cggtgaccga gccggacgcc acgtcgacgg caaggacgag tccgtcggtc gggcagacga
     3721 catagagccg atcgttctcg atgttcgcgg gtctcgcgag gtcgatacag aatgtgccga
     3781 ggtcgaccgt tgcggcgatc gagccgttct gcaggttcag cccgctgagt gtggtcgcat
     3841 tgcccttgac gacccagagc aggtcgtgac cgacggcgaa gcgcgtctgg tcgctgagcc
     3901 gggacacgcg gacgcgttca cccgtcttgt tcgttcgggg atcgagcgtg acgagccggt
     3961 tctccccgga gcaggtccac agcctggttc cgtcgctggt gagtccttga caggcaggca
     4021 ggtcggagta ccccgtcggc agtgacgcca ccgctctccc gctgttcgcg tcgatgcggg
     4081 acacctgacc gtcgtcacgc ttgacccaca cgtcacccgc ggcggacacc aaccagtccg
     4141 ggtcgggcag cgggatcgtc gccacgaggc
//
LOCUS       DATBFZ010000253        11898 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_498710, whole genome shotgun sequence.
ACCESSION   DATBFZ010000253 DATBFZ010000000
VERSION     DATBFZ010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 11898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11898
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_498710"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="VH419_07815"
     CDS             <1..331
                     /locus_tag="VH419_07815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043557.1"
                     /translation="PREALAGGMVGWAVADSPWAHVLDRDAFGDVLDRVRSLQPTRIF
                     SSHLPVADGTSLDEFLTLLETVPDAEPFMPPDAEQFAQMIQAMAMEQQHVPQQRSEPD
                     VEAPTPA"
     gene            complement(365..1039)
                     /locus_tag="VH419_07820"
     CDS             complement(365..1039)
                     /locus_tag="VH419_07820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043558.1"
                     /translation="MARTPLPDLGRLLYAVRPLPTARAWLVMLAPAVVFAVLGWFAPP
                     QTWWTAAVPAAAYLLFIAALGGAGWIDKHRVYERGVVLGPTWPGATPYVVPLDTIDPT
                     SVTVHHHANRINQRIHQNGQPTMRMAVYSTRAVSFAGLHYDEAGGRTRSDSKAFRAAS
                     TLLGQQPMSGGRTCRWVLGVKDPAPLVAAIMQSFEATGRVAPGAARDVLSTAIEEPSG
                     QPVAIR"
     gene            complement(1049..1822)
                     /locus_tag="VH419_07825"
     CDS             complement(1049..1822)
                     /locus_tag="VH419_07825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043559.1"
                     /translation="MTMTDPAAAQIPTVDEMAVRLLHQGRDEPREVVVLTDADLLALD
                     GVDGEHVAPLPWLGRRDRATKELAAELGWRQLAARGLAAAAYEPEIEVHVSLQVQAAL
                     HMRRAASAIVLAQQDSALGTNTLVIYGQGALGLVAEAVDSYGLHRFSTMSATRGLDAL
                     IDFAVPTGDFAPHDGPQTVYSEQEWTTGADTLGQAASATVLVGISRSSADVVERRLSI
                     YALPGRAVAAAPVEEGLGVRDVTRDTVVDLLRRISGSTA"
     gene            complement(1819..3087)
                     /locus_tag="VH419_07830"
     CDS             complement(1819..3087)
                     /locus_tag="VH419_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043560.1"
                     /translation="MSLFEHYPATPGSVRSAGKAIIHAGSRVSDVAQAVDRAHNGALS
                     VVDGLLAMPLLTAPASVRQSAQSTNQSAILAGGAANLFASNIETYNDGIDKLNERYAA
                     AKASSFHVDADRFFMHGGSQTPAQRQAAYDGAVRNAEAALVSSLTAEKRKLDQQLDQD
                     AEHVSGMLRRGPSAGAIYELAAAGAIPPNVALAYPGIDTSFVKELIKGGTTAWKLYSK
                     VLRIRGNLEAAKLAVAAWNEMDAGRSMIPFARTFDDVVDVFGQLDNGDDYMALYAQMR
                     NAEWLSNAARAGIPMSKFAKGLAGVGILAGGYDLFANPDGHTGARRGIDMGMDVVGIG
                     ASGAALAVATGLLVVNPVGLTVIAVAGGVTAAWAAGNYIYDHWDDITAGVDAAKDWVG
                     DRASDVVDKAGDVVDDIGDGISGAWHGVFG"
     gene            complement(3180..3614)
                     /locus_tag="VH419_07835"
     CDS             complement(3180..3614)
                     /locus_tag="VH419_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043561.1"
                     /translation="MTSTSQPPGQRRSALLGAVAAIVLAGTLAGCGSDEPNCAGVAAG
                     CRDSGPSTSANGCVGEPGKESILQGQPHQLPDGAGVGIGSVDMDADPPTVRLVLGNVP
                     ASQRDQDTTVGVGDSLDIGKTTYVVASICSDKVTLDSASPAA"
     gene            complement(3611..3865)
                     /locus_tag="VH419_07840"
     CDS             complement(3611..3865)
                     /locus_tag="VH419_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043562.1"
                     /translation="MSLPETVRLRRGVPTRVQADGREYSLGINAVHDGGRSARIGVFV
                     AGVGETTEVQLGSSVHVGAHAYAVSEIDDRHVVLALESVP"
     gene            complement(3898..5673)
                     /locus_tag="VH419_07845"
     CDS             complement(3898..5673)
                     /locus_tag="VH419_07845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043563.1"
                     /translation="MSLFEHYKATPASVLGQREAALSSVQQLTGIRDDVRRQHEKAMG
                     GILGSLLGAVATTFDPAYQDAEQMARLKLISSASLLEWASDITHYDTAIDGLNERWER
                     ASATSFGVDADQYWMHGADIPAAERQRRFDTAVSNARAALLAQLRREKQTLDAGLDDH
                     ADEAKAILNKDPSDTTLQSLFQAGLFPLQISLLFPSIDIGKVDIAKLIDNLKEHGLLP
                     SSTDTRNIANLWRIISDGADDPDAFQDAYPELRNGLAAASPDGLAFIFSLLTPDQIRR
                     FDQRLTHGVDGLDDSQFYRLDIYAPILSKLPPDLVTKLVGSVATLNPGFDHTDGYLDG
                     NAPQRSDGTDGWHWGDPTGPLFADDDDGDPTNDYLNIQQGGLGDCWMITNMIASTLKN
                     PSFPTDHVAANPNGTITVTLYDHQGVAHQVTVTNQIVLDGNGHSALAHSSDGSTWATY
                     YEKAMALAYGDDDGGAPDGHGGDDTYDRVDNGNYAGLEWDYARNAAPYVSDYGGEEID
                     TNYDTVHHAFVDENHPVMVATKSDDTGLPDDLEDSYHTRHVYYVKGFEDGRIILGNPW
                     GPGYPDVRATPEEFEDWFQQASTID"
     gene            5864..6193
                     /locus_tag="VH419_07850"
     CDS             5864..6193
                     /locus_tag="VH419_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043564.1"
                     /translation="MDTDVVFDAGAGAAKLGPAAAQIATSALHGYDTAAGVVHHPVLT
                     AAFHAFADTHAKLHHSFASRVTDGGARLAKGSNAVSDAENDATHLHQQSFSVAEHVRH
                     ENGSALR"
     gene            complement(6253..8730)
                     /locus_tag="VH419_07855"
     CDS             complement(6253..8730)
                     /locus_tag="VH419_07855"
                     /inference="COORDINATES: protein motif:HMM:NF042457.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6571 family protein"
                     /protein_id="HEY7043565.1"
                     /translation="MHEMFAYYPVSSGVVRDAAEDTRSKGDAVHALMGKVEADHQAAV
                     QAVEGAMRYTVVSTPQGAQQAAQDVGRKTAWASCQLEDFADAVDTFDYNSTDPPSVSK
                     LNTTWSVAAANHFGVSAVHYPDNATPEQKTAADNQHATDVSNASSALLRDLQAKYARL
                     EDNLDQAAERVSSNLQREPTDAELRDAWKAGNLPMTAMLLWPHLGLDLSQINGLPPEL
                     QAMTYDERAQWLIDHPNEGKQLLPHLDTLTKEIVGQKLADQADDVDSDTGRRQLEALT
                     TLLTTYGVDATIARSFTMTLGGPKVLDLVERLGNNVYNHPSGRAVLGLAQAIKATVER
                     GANAMTDDEQRAYAKSLTDAIADQEMSNRTLPLSYLLRDGHFGTAFLDTMGDELDHME
                     RSAERLGFWRSLASADRGVTWLFGDDKTEAAFDPVASYMSALGNNGDAALQFFTEGDG
                     RKEYWIQHRVWSHDDFDGLLSALDAATTSPDTVNDVEAHRLVSETVDYLANRDKLDWG
                     GTSGEGFHPGDVGDSGTRHLAHLIGSYMPSVDVALQERLPDGSQLGENGLQSITDDRI
                     GRLSNMPIFEASELKQLVNVAVSTDDGFMEMRDRVSLYQNVQLNNSVSTHGYVDNQTA
                     NADARIEGFFVNAVGDTKIAEAAEQDARVQSWIDLGKDAVGAIPVPGGKVVEFLASHA
                     IDHGANALSDAYANAESHTVVSENNYATAALAARHQAMEQALVNGGAITPQDVAANLS
                     GVTDSHGHQYTIDDVNRWMPGGHYPTPDQYQAMSPQERNAFESAMRTLSNRELPNGQP
                     LVDTTNYDQSYKDMFSEYFEKGDGDGN"
     gene            8938..9291
                     /locus_tag="VH419_07860"
     CDS             8938..9291
                     /locus_tag="VH419_07860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043566.1"
                     /translation="MSNNAVGLEQVGGVSQAAKGMVENADSRVQSAKTLQNVSEATNG
                     RMVGPAGDMMRNAMTANAGASGTAGVRGAEVATSFAQGEKAVVQGTTDSLQQQKVSAQ
                     EGDNTFQHLSKPIEA"
     gene            9338..9643
                     /locus_tag="VH419_07865"
     CDS             9338..9643
                     /locus_tag="VH419_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043567.1"
                     /translation="MSGMKVRHDFAAGEETISAGSTHHGSLTEARDQSSTLHKTNQAA
                     LGDDVAGSEQVASIAKQQFERDTTSIDSASKMLSSNQRSQDIQQSAAQQATKYFGNR"
     gene            9742..10086
                     /locus_tag="VH419_07870"
     CDS             9742..10086
                     /locus_tag="VH419_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043568.1"
                     /translation="MSYYAMDTDVVQNTGRKVFDLGPEADSAVQGVLNAYMDASSAVH
                     HPLVSQAMSSYHDSHQKAHRAFPQAVRAVGSNTASGGRAIADGQNESTSVQHANALQQ
                     QSLARDLNHHLG"
     gene            complement(10461..>11898)
                     /gene="eccCb"
                     /locus_tag="VH419_07875"
     CDS             complement(10461..>11898)
                     /gene="eccCb"
                     /locus_tag="VH419_07875"
                     /inference="COORDINATES: protein motif:HMM:TIGR03925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type VII secretion protein EccCb"
                     /protein_id="HEY7043569.1"
                     /translation="SSLCLRHAPSDMVFYCLDFGGGTLRALTEVPHVATVTPRTDVER
                     VTRTVNDVLTQLEGRDALFREHGVSSIEEFRRAKAAGRIQADAPGDIFLVVDGWGSFR
                     EEYDALDYTIADIAARGLNYGVHVVITVSQSMEVRMRMQPSFGGRIELRLNDVFDTDF
                     GRAVAEQITKETPGRGAVEPGLIFQAALPRIDGVDDDEDLSDGQSALVQAIAEKWPGR
                     AVARVQVLPEQVLRSELPPVEREYPEIPVGVLDRNLRAASVDLLGGSSAHLLVFGDGE
                     TGKTNLLKLLLKGYMELRTPDELGIVVVDNRRSLLDVVPQEYLLAYSTDNEQTLGVST
                     EIAQSLRKRLPGKDVTSQQLRDRSWWKGLEVLFVVDDYDLVSTSSGNPLTPLIDYFAQ
                     GMDLGFHFVMARRMGGFARAQFEPMVQRLTDLSVPGFIFSGDRMEGRVIGGVTAQRLP
                     LGRALYANRNGLVGQVQTALDDSDPPHP"
BASE COUNT         1816 a         3999 c         4035 g         2048 t
ORIGIN      
        1 gcctcgcgag gcgctcgcag gaggaatggt cgggtgggcg gtcgcggact caccatgggc
       61 tcacgtcctc gaccgcgatg ccttcggcga cgtgctcgac cgggtccgga gtctgcagcc
      121 gacgcggatc ttctcctcac atctgcccgt ggcagacggg acgtcgctgg acgaattcct
      181 cacgctgctc gagaccgtcc ccgacgccga accgttcatg ccaccggacg cggagcagtt
      241 cgctcagatg atccaggcca tggccatgga acagcagcac gtcccacaac agcggagcga
      301 gccggacgtc gaggctccta cacctgcctg accaggcggg tgcgacctgg gcggtggccg
      361 aaggtcagcg aatggcgacc ggctggccgc tgggttcttc gatagcagtc gacaagacgt
      421 cgcgtgctgc gcctggcgcg acccggccgg tcgcctcgaa cgactgcatg atcgcggcca
      481 cgagcggtgc agggtccttg actcccagca cccaccggca ggtgcgccca cccgacatcg
      541 gttgctggcc gagcaatgtc gacgccgctc tgaatgcctt cgaatcggac cgcgtgcgtc
      601 cgccggcctc gtcgtagtgc agcccggcaa agctgacggc ccgggtcgag tagaccgcca
      661 tccgcatcgt cggctgcccg ttctggtgga tccgctggtt gatccggttc gcgtggtgat
      721 gcacggtgac gctggtcggg tcgatcgtgt ccagcgggac gacatacggc gtggcaccgg
      781 gccaggtggg gccgaggacg acgccgcgtt cgtaaacgcg gtgcttgtcg atccagccgg
      841 caccgccgag agcggcgatg aacaggaggt acgccgcagc cggcaccgcg gcagtccacc
      901 acgtctgtgg cggcgcgaac catcccagga cggcgaacac aacggccggc gcgagcatca
      961 cgagccaggc gcgggcggtg ggaagtggcc ggacggcata caagagccgc cccaagtcgg
     1021 gcagcggggt gcgtgccatg cgttgaccct acgcggtgga gccggagatc ctccggagca
     1081 ggtccacgac agtgtcgcgg gtgacgtcgc gtacgccgag cccttcctcg accggagccg
     1141 ccgcgacggc gcggcccggc aacgcgtaga tcgagagccg gcgctcgacg acgtctgcac
     1201 tcgaccggct gatccccacc aagacggttg ccgacgctgc ctgtcccagt gtgtcggcgc
     1261 cagtggtcca ctcctgctcg ctgtagaccg tctgcggccc gtcgtgtggc gcgaagtcac
     1321 cggtgggcac ggcgaagtcg atcagtgcgt cgagacctcg tgttgccgac atcgtgctga
     1381 accgatgcag cccatagctg tcgacagcct cggccaccag accgagcgcc ccttgcccgt
     1441 agatcaccaa ggtgttggtc ccgagcgcgc tgtcctgctg ggcgagcacg atggcggagg
     1501 cagcgcgccg catgtgcagt gcggcttgta cctggaggct cacgtgtacc tcgatctccg
     1561 gctcgtacgc tgccgccgcg agaccacggg cggccagctg ccgccagccg agctcggccg
     1621 ccagctcctt cgtggcgcgg tcgcgacgac caagccaggg gagcggggcg acgtgttccc
     1681 cgtcgacacc gtccagggcg agcaggtcgg catcggtgag gaccacgact tcgcggggct
     1741 cgtcgcgacc ctggtgcagc aaccggaccg ccatctcgtc gacggtgggg atctgcgctg
     1801 cagcaggatc tgtcatcgtc acccgaacac tccgtgccac gcgccgctga tgccgtcacc
     1861 gatgtcgtcc accacgtcgc cggccttgtc gacgacgtcg gacgcccggt cgccgaccca
     1921 gtccttcgca gcgtcgacgc cggcggtgat gtcgtcccag tgatcgtaga tgtagttgcc
     1981 ggcagcccat gccgcggtca cgccgccggc gacggcgatg acggtgagac caacagggtt
     2041 gaccaccagc agacccgtcg cgactgccag agccgctccg gacgcgccga ttcccacgac
     2101 gtccatgccc atgtcgatcc cgcggcgtgc gccggtgtgt ccatcagggt tggcgaagag
     2161 gtcgtagcca ccggccagga tgccgacgcc ggccaggccc ttggcgaact tcgacatcgg
     2221 aatgcctgcc cgggcggcgt tggagagcca ttcggcgttg cgcatctgcg cgtacagcgc
     2281 catgtagtca tcgccgttgt cgagctggcc gaagacgtct acgacgtcgt cgaacgtgcg
     2341 cgcgaacggg atcatggaac ggcccgcgtc catctcgttc cacgcggcga ccgcgagctt
     2401 ggcagcctcg aggttgcccc ggattcggag caccttcgag tacagcttcc acgccgtcgt
     2461 accgcccttg atgagctcct tgacgaacga cgtgtcgata cccgggtagg cgagtgcgac
     2521 attcggcggg atcgcgccgg ctgcggccag ttcgtagatg gctcctgccg aaggaccccg
     2581 acgcagcatg cccgagacgt gctcagcgtc ctggtcgagc tgctggtcga gcttgcgctt
     2641 ctcggcggtg aggctggaga ccagggcggc ctcggcgttg cgcaccgcac cgtcgtacgc
     2701 cgcctgccgt tgtgcaggcg tctggctgcc cccgtgcatg aagaacctgt ccgcgtcgac
     2761 gtggaacgag ctcgccttgg cggcggcata ccgctcgttg agcttgtcga tcccgtcgtt
     2821 gtaggtctcg atgttgctcg caaacaggtt ggccgcacca ccagccagga tcgcggactg
     2881 gttcgtgctc tgggccgact gccgcacgct ggcgggcgcg gtgagcagcg gcatggcgag
     2941 caggccgtcg acgacagaaa gcgcgccgtt gtgagcccgg tcgaccgcct gggcgacgtc
     3001 gctgacgcgg ctgcctgcgt ggatgatggc cttgccggcg ctgcggacgc tccctggcgt
     3061 tgccgggtag tgctcgaaaa gactcatcgt cgcctcgtcc actcgagagg tctgaaacgt
     3121 tgcggcatca gtatcgagcc tgaaggggtc actgcgtgtc ccacccagtc cggccggtgt
     3181 caggcggcag gggaggcgga gtcgagggtc accttgtcgg agcagatcga ggcgacgacg
     3241 tacgtcgtct tcccgatgtc gagggaatct cccactccga ccgtcgtgtc ctggtcgcgt
     3301 tgcgaggcgg gcacgttgcc gaggacgagc cggaccgtgg gtggatcggc gtccatgtcg
     3361 acggagccga taccgacgcc ggcgccgtcg gggagctggt gcggttgacc ttggaggatc
     3421 gactccttgc cgggctcgcc gacgcagccg ttggccgagg tcgacggacc ggagtcgcgg
     3481 catccggcgg cgactccggc gcagttcggc tcgtcgctac cgcaaccggc aagtgtccct
     3541 gcgagcacga tcgcggcaac cgcaccgagc agggcggagc gtcgttgccc tggcggctgg
     3601 gatgtcgacg tcatgggacg gactcgaggg cgaggacgac gtgccggtcg tcgatctcgg
     3661 acacggcgta cgcatgagcg ccgacgtgca ccgacgaccc gagctgcact tccgtcgttt
     3721 cgccgacgcc cgccacgaac acgccgatcc gtgcactgcg tccgccgtcg tgcactgcgt
     3781 tgatgccgag ggagtactcc ctaccgtccg cctggacgcg ggtagggacg ccgcggcgca
     3841 gccgaacggt ctccggcagg ctcatcgatc agtccttccg cgtggtgcgc tgctacgtca
     3901 gtcgatggtc gacgcttgct ggaaccagtc ctcgaactcc tcaggtgtgg ctctcacgtc
     3961 agggtagccg ggcccccacg ggttcccgag aatgatccgg ccgtcctcga agcccttcac
     4021 gtagtacacg tgccgcgtgt ggtagctgtc ctcgagatcg tccggcaggc cggtgtcgtc
     4081 ggacttggtg gccaccatca ccgggtggtt ctcgtcgacg aacgcgtggt ggacggtgtc
     4141 gtagttggtg tcgatctcct ccccgccgta gtccgagacg tacggcgccg catttctggc
     4201 gtagtcccac tcgagtccgg cgtagttgcc gttgtcgacc cggtcgtacg tgtcgtcacc
     4261 gccgtggccg tcgggtgcgc cgccgtcgtc gtcgccgtag gcgagtgcca tcgccttctc
     4321 gtagtacgtc gcccaggtgc tgccgtcaga ggagtgcgcc agcgcggagt ggccgttgcc
     4381 gtcgagcacg atctggttcg tcacggtgac ctgatgagcc acgccctggt ggtcgtacag
     4441 cgtcaccgtg atggtgccgt tcgggttcgc ggccacgtga tctgtaggga agctcgggtt
     4501 cttgagcgtc gacgcgatca tgttggtgat catccagcag tccccgagac cgccttgctg
     4561 gatgttcagg tagtcgttcg tcgggtcgcc gtcgtcgtca tcggcgaaca gcggaccagt
     4621 cgggtcgccc cagtgccagc cgtctgtgcc gtccgagcgt tgcggagcgt tgccgtcgag
     4681 gtacccgtcg gtgtggtcga accccgggtt cagcgtggcg acgctgccga cgagcttcgt
     4741 caccaggtcc ggcggcagct tggacaggat cggggcgtag atgtccagcc ggtagaactg
     4801 gctgtcgtcg agaccgtcga cgccatgcgt gaggcgctgg tcgaaccgcc ggatctggtc
     4861 cggcgtcagg agactgaaga tgaatgccag accgtccggc gacgcagcgg cgagcccgtt
     4921 gcgcagctcc gggtaggcgt cctggaacgc gtccgggtcg tcggcgccgt cgctgatgat
     4981 cctccacagg ttggcgatgt tcctcgtgtc ggtgctggac ggcaggagcc cgtgctcctt
     5041 gaggttgtcg atgagcttgg cgatgtccac cttgccgatg tcgatcgacg ggaagagcag
     5101 gctgatctgc aacgggaaca gcccggcttg gaacagcgac tgcagcgtgg tgtcgctggg
     5161 gtccttgttc aggatcgcct tcgcctcgtc ggcgtggtcg tcgagcccgg cgtcgagcgt
     5221 ctgcttctcc ctccggagct gcgcgagtag cgcggctcgg gcgttgctca ccgcggtgtc
     5281 gaaccgacgc tgtcgttccg cggccgggat gtcggccccg tgcatccagt actggtcggc
     5341 gtcgacgccg aatgacgtcg cgctggcgcg ttcccagcgt tcgttcagtc cgtcgatcgc
     5401 agtgtcgtag tgggtgatgt ccgacgccca ctccagcagc gacgccgacg agatgagctt
     5461 gagccgggcc atctgctcgg cgtcctggta cgccgggtcg aacgtcgtcg cgactgcgcc
     5521 gagcaacgat ccgagaatgc cgcccatcgc cttctcgtgc tgcctgcgaa cgtcgtcgcg
     5581 gatccccgtc agctgctgga ccgagctcag cgccgcctcc cgctgtccga ggaccgacgc
     5641 cggggtcgcc ttgtaatgct cgaacaggct cactgggtcc ccttggtgcc ctcgacggct
     5701 ggctgcgtcg ctcagactag ggcgcgggcg ggtcaccgcc aggcgtcacg tcagagcgat
     5761 ctcggtgccg tcccggtgcc gtctcggtcc aacggtggcc ctcttgtggc gtagcctcct
     5821 cccaacgcag ccgatcggaa ggcggacagt gaacggtttc ggcatggaca ccgacgtcgt
     5881 cttcgacgcg ggtgccggcg ccgcgaagct gggaccggcg gctgctcaga tcgcgacctc
     5941 cgctctccat ggctacgaca cggccgccgg cgtcgtccac catccggtgc tcaccgcagc
     6001 gttccacgcc tttgccgaca cgcacgccaa gctgcaccac tcgttcgcgt ctcgcgtcac
     6061 cgacggggga gcacgcttgg cgaagggcag caacgccgtg tccgacgcag agaacgacgc
     6121 gacgcacctg caccagcaga gcttcagcgt ggccgagcac gtccggcacg agaacggctc
     6181 ggccctgcgt tgaccctggg ctgaccttgg ggcgtccctg ggctgaccct gggctgacca
     6241 agggcgcgcc gctcagttgc cgtcgccgtc gcccttctcg aagtactccg agaacatgtc
     6301 cttgtagctc tggtcgtagt tcgtggtgtc gaccagtggc tggccattcg gcagttcgcg
     6361 gttcgacagc gttcgcatcg cgctctcgaa cgcgttccgc tcctgcgggg acatggcctg
     6421 gtactgatcc ggtgttgggt agtggccgcc aggcatccag cggttgacat cgtcgatcgt
     6481 gtactgatgc ccgtgtgagt ccgtcacgcc tgagaggttg gcggcgacgt cctgaggcgt
     6541 gatcgccccg ccgttcacga gtgcctgctc catcgcctgg tgtcgggccg cgagcgcggc
     6601 ggtggcgtag ttgttctcgc tcacgaccgt gtggctctcc gcgttggcgt aggcgtccga
     6661 cagggcgttc gcgccatggt cgatcgcgtg actggcgagg aactcgacca ccttcccgcc
     6721 gggcaccggg atggccccga ccgcgtcctt gcccaagtcg atccacgact ggacccgggc
     6781 gtcctgctcg gctgcctccg cgatcttggt gtcgccgacc gcgttgacga agaacccctc
     6841 gatgcgggca tcggcgttgg ccgtctggtt gtcgacgtac ccatgcgtcg agacgctgtt
     6901 gttgagctgc acgttctggt acaggctcac ccggtcgcgc atctccatga agccgtcgtc
     6961 ggtggacacc gcgacgttga cgagctgctt caactcgctc gcctcgaaga tcggcatgtt
     7021 ggacaaccgg ccgatccggt cgtcggtgat cgactgcagt ccgttctcac ccagctggct
     7081 gccgtccggc aacctctcct ggagagcaac gtcaaccgac ggcatgtagc tgccgatcag
     7141 gtgtgcgaga tgcctggtgc cgctgtcacc gacgtcgccg gggtggaacc cttcgccgct
     7201 tgttccgccc cagtcgagct tgtcgcggtt ggcgaggtag tcgaccgtct ccgacaccag
     7261 tcggtgcgcc tcgacgtcgt tgaccgtgtc cgggctggtc gtcgcggcgt cgagggccga
     7321 cagcaaaccg tcgaagtcgt cgtgactcca cacgcggtgc tggatccagt actccttccg
     7381 accgtcacct tcggtgaaga actgcagggc ggcgtcgccg ttgttgccga gggctgacat
     7441 gtatgacgcc accgggtcga acgccgcctc ggtcttgtca tcgccgaaca accacgtgac
     7501 cccgcggtca gcgctcgcaa gactgcgcca gaagccgagt cgctccgccg accgttccat
     7561 gtggtcgagc tcgtcaccca tggtgtccag gaacgccgtc ccgaagtggc cgtcacgcag
     7621 cagatacgac agtggcaacg tccggttcga catttcctgg tcggcgatcg cgtccgtcag
     7681 gctcttggcg taggcgcgct gctcgtcgtc ggtcatcgcg ttcgcgcccc gctcgacggt
     7741 cgccttgatg gcctgtgcca ggcccaggac ggcgcggccg ctcgggtggt tgtacacgtt
     7801 gttgccgagc cgttcgacca ggtcgagcac cttcggcccg ccgagcgtca tggtgaacga
     7861 ccgggcgatg gtggcgtcga cgccgtacgt cgtcaacaac gtggtcagtg cctcgagctg
     7921 gcgccttccg gtgtcgctgt ccacgtcgtc ggcctggtcg gcgagcttct gcccgacgat
     7981 ctccttggtc agcgtgtcga ggtgcggcaa cagctgcttc ccctcgttcg ggtggtcgat
     8041 cagccattgg gcgcgctcgt cgtacgtcat ggcttggagc tcgggtggca ggccgttgat
     8101 ctgcgacagg tcgaggccga ggtgcggcca caggagcatg gcggtcatcg ggaggttgcc
     8161 ggccttccac gcatccctga gctcggcgtc ggttggctca cgctgcaggt tgctggagac
     8221 ccgctcggcc gcctggtcga ggttgtcctc gagccgggcg tacttcgcct ggaggtcccg
     8281 cagcagtgcg ctcgaggcgt tgctcacgtc ggtcgcgtgc tggttgtcgg cggcggtctt
     8341 ctgctcaggc gtcgcgttgt ccgggtagtg caccgcagac acgccgaaat ggttggcggc
     8401 agcgacgctc cacgtcgtgt tgagcttcga caccgagggc ggatcagtgc tgttgtagtc
     8461 gaacgtgtcg acggcgtccg cgaagtcctc gagctggcag gatgcccacg ccgtcttgcg
     8521 gccgacgtcc tgagcggcct gttgtgcgcc ctgcggcgtc gacacgacgg tgtagcgcat
     8581 ggccccctcg accgcttgca ccgccgcctg gtggtcggcc tccaccttgc ccatcagcgc
     8641 gtggacggca tcccccttgg aacgcgtgtc ctcggccgcg tcccggacga cgccggagct
     8701 cacagggtag tacgcaaaca tctcgtgcat ggagtctctg cctcacgttg gacttcgacg
     8761 gacgacgaac agtgacggta ctgatcggga gggtcaccag cggcgtacca gcccgcatcg
     8821 tcggcgctgc tcgagcgtcg gaccgagcgg attcggggcg gtgaccccct gcggctgtag
     8881 cgtccttctc gtcgtcggcg aagggtcggc gggaagtcga aaactcctgg aggagaaatg
     8941 tcgaacaacg cagttggtct ggagcaggtt ggtggtgtct cccaggctgc gaagggcatg
     9001 gtggagaacg ctgactcccg tgtccagtcc gccaagacgc tgcagaacgt ttccgaggca
     9061 accaacggcc gtatggtcgg tccggccggc gacatgatgc ggaacgcgat gaccgccaac
     9121 gccggcgcgt cgggaaccgc cggtgtgcga ggcgccgagg tcgcaaccag cttcgcccaa
     9181 ggtgagaagg ccgtcgttca gggcaccacg gactcgctgc agcagcagaa ggtgtcggcc
     9241 caagagggcg acaacacgtt ccagcacctc agcaagccca tcgaagcctg acggctgcca
     9301 cggatcacga ccgatctcga cgtaaaggaa accgatcatg agtggaatga aggttcgtca
     9361 cgacttcgcc gccggcgaag agacgatcag cgccggctcg acccaccacg ggtcgctcac
     9421 cgaggcacgt gaccagtcgt cgacgctgca caagaccaac caggcggcgc tcggtgacga
     9481 cgttgccggc tccgagcagg tcgcaagcat cgccaagcag cagttcgagc gcgacacgac
     9541 gagcatcgac tcggcgtcca agatgctgag cagcaaccag cgcagccagg acatccagca
     9601 gtccgccgcc cagcaggcca ccaagtactt cgggaaccgc tgagccgagg ctgccgggct
     9661 cgttcagccc ggcagccatc tggcgtacgc cgccgaagcc agcggcgtga tcgatcgacg
     9721 cgacgagcca ggagcacgca gatgagttac tacgccatgg acaccgacgt ggtccagaac
     9781 accgggagga aggtcttcga cctcggcccg gaggcggact ctgccgtcca aggcgtgctg
     9841 aacgcctaca tggacgcgtc ctcagcggtc caccacccgc tcgtcagcca ggcgatgagc
     9901 agctaccacg actcgcacca gaaggcgcac cgcgcgttcc cgcaagcggt ccgggcggtg
     9961 ggctccaaca ccgcgtccgg cggtcgggcc atcgcggacg gccagaacga gtcgacctcc
    10021 gttcagcacg ccaacgcact ccagcagcag agcctcgccc gcgacctcaa ccaccacctc
    10081 ggctgatcct gaccccacgc cgggcgtttg cccggttcgc catacgaccg cacgtttcgc
    10141 caggcggacc tgggttctcg tcggcctctg tggcggcgat ggtcgctatg aatgaccgtg
    10201 gtcgcccggc gctcgtcggc ttcggctgag gcgggtcggc agcgaagagc gggaagcgtt
    10261 cctgcccact gaggcgatcg gccgccgtcc cgtccttgtc ggtgggtgcg tgacgggcgg
    10321 gtctgcgtag atccgccggt cgcggtgcgg cggtttgcat ggctacccac gcaacccgac
    10381 ccctcccaag gcagatctgc ctggctaccc gtccgtttgc atggctaccc aggcaaacca
    10441 ccagggacgg gtggcgaggg tcaggggtgt ggcgggtctg agtcgtcgag ggccgtctgg
    10501 acctggccga ccaggccgtt ccggttggcg tacagcgccc ggccgagcgg gagtcgctgc
    10561 gcggtgacac ccccgatcac gcgtccttcc atccggtcgc cggagaagat gaagcccgga
    10621 acgctcaggt cggtcagccg ctgcaccatc ggctcgaact gggcgcgcgc gaagccgccc
    10681 atgcgcctcg ccatgacgaa gtggaacccg agatccatgc cctgcgcgaa gtagtcgatc
    10741 aacggggtga gcgggttccc ggaggaggtc gacaccaggt cgtagtcgtc gaccacgaag
    10801 agcacctcga gccccttcca ccagctgcgg tcccgaagct gctgcgaggt cacgtccttt
    10861 ccgggtagcc gcttgcgcaa cgactgcgcg atctcggtcg acacgcccag ggtctgctcg
    10921 ttgtccgtgg agtacgcgag caggtactcc tgcggtacga cgtccagcag gctgcgccgg
    10981 ttgtcgacga cgacgatgcc gagctcgtct ggcgtcctga gctccatgta gcccttgagc
    11041 agcagcttga gcaggttcgt cttacccgtc tcaccgtcac cgaacaccag caggtgcgcg
    11101 gagctgccgc ccagcaggtc gaccgacgcc gcacggagat tccggtcgag tacgccgaca
    11161 gggatctccg gatactcccg ctcgaccggc ggcagctcgc tgcgcagcac ctgctccgga
    11221 agcacctgga cgcgcgccac cgccctgccg ggccacttct ccgcgatcgc ctggaccagc
    11281 gccgactgtc cgtcggagag gtcctcgtcg tcatcgacac cgtcgatccg cggcaacgcc
    11341 gcctggaaga tcagaccggg ctcgaccgct ccgcgtccgg gggtctcctt cgtgatctgc
    11401 tccgccacgg cccgaccgaa atcggtgtcg aacacgtcgt tgagccgcag ctcgatgcgc
    11461 ccgccgaacg acggctgcat ccgcatccgt acctccatcg actggctgac cgtgatcacc
    11521 acgtgcacgc cgtagttgag accacgtgcc gcgatgtcgg cgatggtgta gtccagcgcg
    11581 tcgtactcct cccggaacga accccagccg tccacgacca ggaagatgtc gccgggtgcg
    11641 tcggcctgga tccggccggc cgccttcgcc cggcggaact cctcgatgct cgacacgcca
    11701 tgctcgcgga agagcgcgtc gcggccctcg agctgggtga gtacgtcgtt cacggtgcga
    11761 gtcacccgct ccacatccgt gcgcggcgtc accgtggcga cgtgcggcac ttcggtcagg
    11821 gcacgcagcg ttccgccacc gaagtcgagg cagtagaaaa ccatgtccga cggcgcgtga
    11881 cgaaggcaca gcgacgag
//
LOCUS       DATBFZ010000254         3683 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_2791373, whole genome shotgun sequence.
ACCESSION   DATBFZ010000254 DATBFZ010000000
VERSION     DATBFZ010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 3683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3683
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2791373"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            415..825
                     /locus_tag="VH419_07880"
     CDS             415..825
                     /locus_tag="VH419_07880"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HEY7043570.1"
                     /translation="MSTATIDIADHRDTAHHRPVRRVSSAGETAALRRSARASAPLRL
                     TRRGRLVVLIAVLAVTFAVFTFVGGPAVSTGDTHHAAQHTVVVASGETLWDIASRIAP
                     AKDPRSVIADIVDLNNLADAGSIRVGQQLFVPAY"
     gene            complement(841..2418)
                     /locus_tag="VH419_07885"
     CDS             complement(841..2418)
                     /locus_tag="VH419_07885"
                     /inference="COORDINATES: protein motif:HMM:NF014214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M36 family metallopeptidase"
                     /protein_id="HEY7043571.1"
                     /translation="MLRLSSFSSRRSRRCVAVLGAAALTAVSLTTAAVGQETSAVSRA
                     AGPLAGSAALSQRESVRVPGNVRFYQERTSLLGRHRWYQQLANGHVVVGGWYAVHLWN
                     DGTTTVWDGRKNVRGLAKTPATISADAAAKHVDKVDAAATLQTDTDGLMVLPSDDGRP
                     AQLVWRVVGVDGSGVTASYVDATTGVTLETEQLAKNLVDPQGLGRLDRRAPAHALVTG
                     RARVFDPNPVVALQREGLRDRRDANTAVPRTAYRIVDLPRLRHRHTLVGRFVRIVNPD
                     RATSAVDRYFFLRHNDRFEQVNAYYAVDAEQAYLHRLGFTDVNADAQDVATDAFPDDN
                     SFYNPADDLISLGTGGVDDAEDPEVIWHEYGHAIQDDQVPDFGRSFQAAAMGEGFGDY
                     MAVVTSQLAPHPNDTSRTPAACVMDWDSTSYTTDTPHCLRRTDTDLHYPEDLHRVDPH
                     FDGQIWSGALWDLAQALGQDKATMLVVEAQFAMNPAIAMPAAARAVVATTTALSPGDA
                     ATTRQVFVDRGILPSTS"
     gene            complement(2930..3358)
                     /locus_tag="VH419_07890"
     CDS             complement(2930..3358)
                     /locus_tag="VH419_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043572.1"
                     /translation="MLAAVRRIGVLPSHVEGPRFTLVNLETTFFTTPHPVSRSLTLIG
                     FDVDVRVTPTSYSWHWGDGTSQQTSSPGRPFPARDVTHVYRQHTDNGAPLALRVDTVY
                     TARYRVDGGPWQQIPEALTIPGPATALPVKQASAVLVAGD"
BASE COUNT          559 a         1210 c         1291 g          623 t
ORIGIN      
        1 ctgcccggtc gcgggggcgg gcgatctcgg cgacttcgcg gtcttggacg tcttggccat
       61 ctcgtcacgc cttcgttcgg tccggtcgtc tcgcagacga cggtagacct gacggccgac
      121 gctttcaaac acctgttcga atcggcgtgt cctggcgatg tcgaggggca ggctctcggt
      181 tgggccgcgg ctcgaacacg cgttcgatca atgcgcacca ttgtcggtgg cccgcgctat
      241 cgtttcaaac atgtgttcga tcgaacgtgt cttcgaatcc gggcgccgcc ggtggccgcc
      301 gccgctcgtc ggtcggcggg cagtgtcggt gttcggcggt acaacgatga ccggtcgtga
      361 cgaccgacgc ccgcactccc gatccctgtc cgactacgac cctggaggtc accgatgagc
      421 accgccacca tcgacattgc cgaccatcgc gacactgccc accatcgccc ggtccgacgg
      481 gtgagttctg cgggagagac cgccgcgctt cgacgttccg cacgcgcatc ggcaccgctc
      541 cggctgaccc ggcggggccg gctcgtcgtg ctgatcgccg tgctggccgt caccttcgcg
      601 gtcttcacgt tcgtcggcgg tccggcggtc tcgacgggtg acacacacca cgcagcgcag
      661 cacacggtgg tggtggccag tggcgagacg ctgtgggaca tcgcgtcgcg catcgccccg
      721 gccaaggacc cacgctcggt gatcgccgac atcgtcgacc tcaacaacct cgcggacgcg
      781 gggtcgatca gggtcggaca gcagttgttc gtcccggcgt actgacggac gagtgcggca
      841 ctacgaggtc gacggcagga tgccgcggtc cacgaagacc tggcgggtcg tggcggcgtc
      901 cccaggcgac agcgccgtgg tcgtggcgac gactgcccgc gcagcagcag gcatcgcgat
      961 ggcggggttc atcgcgaact gggcctcgac gacgagcatc gtggccttgt cctgaccgag
     1021 tgcttgcgcg aggtcccaga gcgccccgga ccagatctgt ccatcgaagt gtggatcgac
     1081 ccggtgcagg tcctccgggt agtgcaggtc ggtgtcggtc cgccgcagac agtgcggcgt
     1141 gtccgtggtg tatgacgtcg agtcccagtc catcacgcag gcggctggtg tcctcgaggt
     1201 gtcgttggga tgaggtgcca gctggctcgt cacgaccgcc atgtagtcgc cgaagccctc
     1261 gcccatcgcc gcggcctgga agctgcgacc gaagtcgggg acctggtcgt cctggatggc
     1321 gtgaccgtac tcgtgccaga tcacttcggg gtcctcggcg tcgtcgaccc cgccggtgcc
     1381 gagcgagatc aagtcgtcgg ccgggttgta gaaggagttg tcgtcgggga acgcgtcagt
     1441 ggctacgtcc tgggcgtcgg cgttgacgtc ggtgaacccg agtcggtgca ggtaggcctg
     1501 ctcggcgtcg accgcgtagt aggcgttcac ctgctcgaac cggtcgttgt ggcggaggaa
     1561 gaagtagcgg tcgaccgcgc tcgtggcacg gtcggggttg acgatgcgga cgaacctgcc
     1621 gaccagcgtg tggcggtggc gcagccgtgg caggtcgacg atccggtacg ccgtccgcgg
     1681 caccgcggtg ttcgcgtccc ggcggtcgcg gagcccttcg cgctggagcg ccacgacggg
     1741 gttcgggtcg aacacccggg cgcgaccggt cacgagcgcg tgggccggtg ctcggcggtc
     1801 gagcctgccg agaccctgtg ggtccaccag gttcttggcg agctgctcgg tctcgagcgt
     1861 cacgccagtc gtcgcgtcga cgtacgacgc cgtgacgcca ctcccgtcga cgccgaccac
     1921 gcgccagacg agctgggcgg gccgtccgtc gtcggagggc agcaccatca gaccgtcggt
     1981 atccgtctgc agggtcgcgg ccgcatcgac cttgtccacg tgcttggccg cggcgtcggc
     2041 ggagatcgtg gccggggtct tggcgagtcc gcggacgttc ttgcggccgt cccacacagt
     2101 ggtggtgccg tcgttccaca ggtgcacggc ataccatccg ccgaccacga catggccgtt
     2161 ggccagctgc tggtaccacc gatggcgacc gagcagcgac gtgcgctcct ggtagaaccg
     2221 cacgttgccc gggacccgga ccgactcgcg ctgcgacagc gcggcgctgc cggccagggg
     2281 accggctgcg cgcgacaccg ctgaggtctc ttggccgacg gcagcggtgg tgagcgagac
     2341 cgcggtcagc gcggcggcgc cgagcacggc gacgcagcgt cgggagcgac gtgatgagaa
     2401 cgaggacaag cggagcatcg gcgacttcct tcgcccggga ccggagacga accgcccaac
     2461 cgtaccccgg tctccctcac cacgcgcgac tgtccacagg agagtgtgga gaaacacact
     2521 cgtccggtgg acaacgaggt gaactcggtg gatgggcagc gctgtttctg tgggtaacca
     2581 cggtggatga aaattacctc gaagttcttt gctgcaaggg gttgtgcgac cagttcgggc
     2641 gcgtgtagaa atctcttcac gccctcccga aagggaaggc gggatcggac ggaaaccgag
     2701 gatccacacg gtgaggcgac tcaccgcagc ggccggtgac gaggctggtg gggtccgacg
     2761 agtcgaccga tcggctggtg acgtcacttc actccggccg acctcgtggg gtccctcaaa
     2821 ctcatacgtt gaggggcccc acacctctga ccgcaccagg ctgggtcatg tcgcggagtg
     2881 gttcatgtcg cccgagcgac gtgaacccac tcctcgttcc tgggtcctgt cagtcacccg
     2941 cgaccaagac cgcggacgct tgcttcaccg gcaacgccgt ggccggtccg gggatcgtga
     3001 gtgcctccgg gatctgctgc cacgggccgc cgtcgacgcg gtagcgcgcg gtgtacaccg
     3061 tgtccacccg cagcgccagc ggtgcaccgt tgtcggtgtg ctgtcggtag acgtgcgtga
     3121 cgtctcgtgc agggaacggg cggccaggtg agctggtctg ctggcttgtc ccgtcgcccc
     3181 agtgccagct gtacgacgtc ggcgtcaccc gaacgtccac gtcgaatccg atgagcgtca
     3241 gcgaacgcga caccgggtgc ggcgtggtga agaacgtggt ctcgaggttc accagggtga
     3301 acctcggtcc ttcgacgtgg gagggaagca cgccgatgcg ccggactgcg gcgagcacgt
     3361 cctgccacgt caacgtccgc cgctggaccg gccccgcatc cgccggatcc ctgcactcgc
     3421 tgtacaacac ccgccacggc cgactggctg accgcccatt ggagtccgtg gtttggcgaa
     3481 cccaaaggct ttgcctggag agatccggat cggcgcatgt gttctgcccc ctgcacgaga
     3541 cagcaggtgc attgcggtct ccgggtaggg cggcaggaca actcggaagc catatgtatt
     3601 cgtatgtacc cccggtgctc gcggcgcccg ggccgggcac gccattcgaa gcgctcgaat
     3661 cgcgtgactc ctggcacttg atc
//
LOCUS       DATBFZ010000255         5074 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_3123819, whole genome shotgun sequence.
ACCESSION   DATBFZ010000255 DATBFZ010000000
VERSION     DATBFZ010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 5074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5074
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3123819"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(62..595)
                     /locus_tag="VH419_07895"
     CDS             complement(62..595)
                     /locus_tag="VH419_07895"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY7043573.1"
                     /translation="MATQQLPSRLHHNAYVTKDQEATRAFYEDIIGLPLVATWSEADE
                     LFGALRVYCHTFFGLGDGSALAFFQFANAEDQEMFDPDLTSSPFRHIALRVTPELQQE
                     IHERLTRSNWKPDGTYVLEHGYCRSLYTEDPNGLLLEFTADVPNADEIAVERRADAHE
                     TLKRWLAGDHTSNNVYR"
     gene            complement(708..1664)
                     /locus_tag="VH419_07900"
     CDS             complement(708..1664)
                     /locus_tag="VH419_07900"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY7043574.1"
                     /translation="MQILGRALAFDPAREAIAAEAAGYDGVRVIDHFFSGIAPEPQRA
                     TSHSIAGLAAAAAVTERVLLTSTMLSAPIRHPLECAQAIATIDRISAGRAELGIGGGW
                     NRAEFAAIGVELGTPRDRVARLVEATTICRQAFEHKGVVHFDGRFFHAHCDAEWPATP
                     HVPEVMVAAGGPGTIRRAAPVANRLDLLESMPEGRPVLDATHVNSEEHLVDRIALARE
                     VAGRAGNDIKLSATVMLSITPDAVTRDRSREQMAEIAQSTPDLLDRELLRVIDTGEGA
                     FGRIRTLAAIGFDRLHIRPADEPTQRWLDAGLADLQRLPTPG"
     gene            1814..2248
                     /locus_tag="VH419_07905"
     CDS             1814..2248
                     /locus_tag="VH419_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043575.1"
                     /translation="MLEATTADWREIADWVTTFGGHVRRREFADAAEQFDPAVVSFSS
                     LRDVVVGLDDLIEHQWQHVWPTIDGFEFDVDALHAFISPDRCLAVAAVTWTSTGFDAE
                     GDPFDRPGRATLVLGRDRPSAPWHGIHAHFSLNRGVPQPTVA"
     gene            complement(2310..2603)
                     /locus_tag="VH419_07910"
     CDS             complement(2310..2603)
                     /locus_tag="VH419_07910"
                     /inference="COORDINATES: protein motif:HMM:NF020317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HEY7043576.1"
                     /translation="MPTFIYLTTWTDQGAQDPKSAVDRASAAVQALESMGVKIFEVYW
                     TNGVYDLVMVAECADEETAYAASLDMASRGNVRPTMLRAFDRGEMTAIVSKLT"
     gene            complement(2582..2923)
                     /locus_tag="VH419_07915"
     CDS             complement(2582..2923)
                     /locus_tag="VH419_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HEY7043577.1"
                     /translation="MTEDWVEAGTVADLQRRKRSVVEADGRQIALFWHDGEVHALDNV
                     CIHKQRELVKGVVLNGRIVCPGHQWAYDLKTGYEQTMDRYQPCFPVKVEDGKVYVTAR
                     ERTDACRPSST"
     gene            complement(2920..3288)
                     /locus_tag="VH419_07920"
     CDS             complement(2920..3288)
                     /locus_tag="VH419_07920"
                     /inference="COORDINATES: protein motif:HMM:NF012339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEY7043578.1"
                     /translation="MPTIQFRTSGRTVAFDDGDDVNLLRTSIRYDCGVPYKCASGNCG
                     TDRVLVEAGADNLSRIRPKERDRLGDDVDRGYRLACQTYVSGDVTISWEVAPVTENQR
                     LRDRWLAGLAGPEREGGGPA"
     gene            complement(3304..4047)
                     /locus_tag="VH419_07925"
     CDS             complement(3304..4047)
                     /locus_tag="VH419_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HEY7043579.1"
                     /translation="MAELLSRDEFRTALEGAIRGREARDASFSQAWANGLLSRDHLAR
                     WAENHYHYVGPFADYLAYIYGNAPDTATDAKDFLLQNMYEEEVSDVRHTDLLIRFAEA
                     CGTTRERVEDPRNMNPVTRGLQGWCYATAFRQHFAVATAALVVGLESQVPSIYRRQYP
                     VLLDKYGFTEEEAEFFELHITSDEVHGERGYQIVLDNATTPELQQRCIEVVQDGAEMR
                     YAYTKALYDTYVAKDLEGVSLTGAEPAIA"
     gene            complement(4248..>5074)
                     /locus_tag="VH419_07930"
     CDS             complement(4248..>5074)
                     /locus_tag="VH419_07930"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043580.1"
                     /translation="QARAASTQRVRAAVGHSMASAMAAVVPSLGVSGGKLYCTYPGGF
                     SFVVALFALDGTVLCTLDGDALTRVRTAAATALAARHLAPAGVRTAALLGTGRQSEWQ
                     ARALAQELDLEELRVWGRTPDRAEAVAASVRADGIPARAVTGADEAVAGAGVVVTVTS
                     SYESLFSGSSLPADALVCGVGSTKADRRELDGDTVKRAQLIVSDSLEGARVEAGDLVQ
                     AAAEGLIDLDDVVELGEVACGAVGRPSSGLVLFESQGIALEDVVGAALAYRSWAES"
BASE COUNT          738 a         1761 c         1743 g          832 t
ORIGIN      
        1 gcgcgccgtg gacgaacagc accgtcgaac cacgggcccc ggccatgagc gagccggccg
       61 gttaccggta gacgttgttg ctcgtgtggt cgcccgccag ccagcgcttc agggtctcgt
      121 gcgcgtcggc ccggcgctcg actgcgatct cgtcggcgtt cgggacgtcg gcggtgaact
      181 cgaggagcaa tccgttcggg tcctcggtgt acagcgatcg gcagtagccg tgctccagga
      241 cgtacgtgcc gtccggcttc cagttggacc tcgtcagccg ctcgtggatc tcctgctgga
      301 gctctggcgt gacccgcagc gcgatgtggc ggaagggcga cgacgtcagg tcgggatcga
      361 acatctcctg gtcctcggcg ttggcgaact ggaagaacgc cagggcgctg ccgtcgccga
      421 gcccgaagaa cgtgtggcag tacacgcgga gcgcgccgaa gagctcgtcg gcttccgacc
      481 aggtcgcgac gagcggcagg ccgatgatgt cctcgtagaa ggcacgcgtc gcctcctggt
      541 ccttggtgac gtacgcgttg tggtgcaatc gactcggtag ctgttgcgtc gccacggttc
      601 ctccatctcg agctgggcgg gaagcgccca ctgaccggac aggttaccgg tgtgcggtca
      661 gcggtacacg ccgccgatgt gacgtgtcga cccgattctc gcgcgggtca ccccggggtc
      721 gggagccgct ggaggtcggc gagcccggcg tcgagccagc gctgtgtcgg ctcgtccgca
      781 ggccgtatgt gcaggcggtc gaacccgatg gcggccagtg tccggatgcg cccgaacgcg
      841 ccctctcctg tgtcgatgac ccgcaggagc tcccggtcga gcagatccgg cgtcgattgc
      901 gcgatctcgg ccatctgctc gcgggaccga tcccgagtca ccgcatccgg cgtgatcgac
      961 agcatcaccg tggccgagag cttgatgtcg ttgccggccc gcccggccac ctctcgggcg
     1021 agggcgatgc ggtcgacgag gtgctcctcg ctgttgacgt gcgtggcgtc gagcaccggt
     1081 cgcccttcag gcatgctctc gaggaggtcc agccggttcg cgaccggcgc ggcgcgcctg
     1141 atcgtccccg gaccacccgc ggccaccatc acctcgggca cgtggggcgt cgccggccat
     1201 tcggcgtcgc agtgcgcatg gaagaaccga ccatcgaagt ggacgacacc tttgtgctcg
     1261 aaggcctggc ggcagatggt tgtcgcctcg acgaggcggg cgacgcggtc gcgcggcgta
     1321 ccgagctcca caccgatggc ggcgaactcc gcccggttcc agccgccgcc gatgcccagc
     1381 tcggcgcggc cggcggagat gcggtcgatg gtggcgatcg cttgagcgca ctccagcgga
     1441 tgccggatcg gcgcggacag catggtggaa gtcaggagca cccgctcggt gaccgcggcc
     1501 gcggccgcca ggccggcgat gctgtgtgag gtggcgcgct gcggttcggg cgcgatgccg
     1561 ctgaagaagt ggtcgatgac ccgcacgccg tcgtagccgg cggcttcggc cgcgatcgcc
     1621 tcgcgagccg gatcgaacgc cagcgcccta ccgaggatct gcatcgtcgt tcagctcacg
     1681 atcgttcgtc cgagacggcg cccgcagcgc cgtgggtgaa tggaatacag tatcccctac
     1741 gagtcggcca caggtaccat gccgccgcgg tggtgctccg gcgcgctgac tctctgggca
     1801 atgggaggcg tgcatgctcg aggcaacgac agcggattgg cgcgagattg ccgactgggt
     1861 gacgaccttc gggggccacg ttcgccgccg ggagttcgcg gacgcggccg agcagttcga
     1921 cccagcggtg gtcagcttct cgtccttgcg cgacgtggtc gtcgggctcg acgatctgat
     1981 cgagcaccag tggcaacacg tctggccgac gatcgacggc ttcgagttcg acgtcgacgc
     2041 gttgcacgcg ttcatctccc ccgatcgctg cctggcggtc gcggcggtga cgtggacgtc
     2101 cactggcttc gacgcggagg gcgacccgtt cgaccggccc ggtcgggcca ccctggtcct
     2161 cggacgcgac cggccgtcgg caccatggca cggcattcac gcccacttct cgctgaatcg
     2221 aggcgtgccc cagcccaccg tcgcatgacg accggcgtat tgagcgcgac gcactttcga
     2281 gtggggcagg ttcgccggct cacggcgcct cacgtcagct tcgacacgat cgccgtcatc
     2341 tcgccacggt cgaacgcacg cagcatcgtc gggcggacgt tgccgcgaga ggccatgtcc
     2401 aaagacgcgg cataggccgt ctcctcgtcg gcgcactccg ccaccatcac caagtcgtac
     2461 acgccgttcg tccagtagac ctcgaagatc ttcacgccca tcgactcgag cgcctgaact
     2521 gcggccgatg cgcggtcgac cgcgctcttt gggtcctggg ctccttggtc ggtccacgtc
     2581 gtcaggtaga tgaaggtcgg catgcgtccg tcctctcccg cgccgtcacg tacacctttc
     2641 cgtcttcgac cttcaccggg aagcacggct ggtagcgatc catcgtctgc tcgtacccgg
     2701 tcttgaggtc gtacgcccac tggtggccgg ggcacacgat ccggccgttg aggacgacac
     2761 ccttcacgag ctcgcgctgc ttgtggatgc acacgttgtc gagtgcgtgg acctcgccgt
     2821 cgtgccagaa cagcgcgatc tggcgcccgt cggcctcgac cacagaccgc ttgcgtcgct
     2881 gcaggtcagc caccgtaccg gcctcgaccc agtcctcggt cacgccggcc cacctccttc
     2941 ccgttcgggc ccggccagcc cggcgagcca gcggtcacgg agccgctggt tctcggtcac
     3001 cggggcgacc tcccacgaga tcgtgacgtc gccgctgacg tacgtctggc acgccagccg
     3061 gtagccgcga tcgacgtcgt cgccgagccg gtcgcgttcc ttggggcgga tgcggctcag
     3121 gttgtccgca ccggcctcga cgagcacgcg gtccgttccg cagttgccgc tcgcgcactt
     3181 gtacggcacg ccgcagtcgt accggatcga cgtgcgcagc aggttcacgt cgtctccgtc
     3241 atcgaaggca accgtcctcc cgctggtgcg gaactggatg gtcggcatcg accccgcgta
     3301 tcgctacgcg atcgcgggct cggcgcccgt gagcgagacg ccttcgagat ccttggcgac
     3361 gtacgtgtcg tagagcgcct tggtgtacgc gtagcgcatc tcggcaccgt cctggacgac
     3421 ctcgatgcag cgctgctgca gctcgggcgt cgtcgcgttg tcgagcacga tctgatagcc
     3481 gcgctcgccg tgcacctcgt ccgaggtgat gtgcagctcg aagaactcgg cttcctcctc
     3541 ggtgaagccg tacttgtcga ggagcaccgg gtactggcgg cgatagatgg acggcacctg
     3601 cgactcgagc ccgacgacga gcgccgcggt ggcgaccgcg aagtgctgcc ggaaggcggt
     3661 ggcgtagcac cagccctgga gcccgcgggt caccgggttc atgttccgag gatcctccac
     3721 ccgctcgcgt gtcgtgccgc acgcttcggc aaagcggatc agcagatccg tgtggcggac
     3781 gtcgctgacc tcttcctcgt acatgttctg gagcaggaag tccttggcgt ccgtcgccgt
     3841 gtccggcgcg ttgccgtaga tgtaggcgag gtagtcggcg aatggtccga cgtagtggta
     3901 gtggttctcg gcccatcggg ccaggtggtc ccgcgacagc agaccgttgg cccatgcctg
     3961 gctgaacgac gcgtctctgg cctctcggcc gcggatcgcg ccttcgagcg cggtccggaa
     4021 ctcgtctctc gacagcagct cggccatggc cgcctccttt cgtggctagt tggatactgt
     4081 atacgactcg cgacggtgcg gcaaggccgg cctcgtcggg ccggcgttgc gttcatgtcg
     4141 cgccatcccg cggtccagcg ttgaaatccg cgacatgaac ggggggcgcg acgcacggtg
     4201 agaccttcgg gagcgcgcac ggccgggttc agggcaccgc tctggagtca cgactccgcc
     4261 cagctgcggt acgccaaggc cgcgccgacg acgtcttcga gcgcgatgcc ctgcgactcg
     4321 aagagcacga gccccgacga cggccgaccc accgccccgc acgcgacctc ccccagctcg
     4381 acgacgtcat ccagatcgat gagcccttcg gccgcggcct gcacgaggtc acccgcctcg
     4441 acgcgtgcgc cctcgagtga gtcactgacg atcagctgcg cccgcttgac ggtgtcgcca
     4501 tcgagctcgc gccggtcggc cttcgtcgaa ccgacaccgc agacgagcgc atcggcgggc
     4561 aacgacgagc ccgagaacag cgactcgtac gagctggtca cggtgacgac gacgcccgct
     4621 ccggcgacgg cctcgtcagc acccgtcacg gcgcgcgccg ggatgccatc ggcccggacg
     4681 gaggcagcga cggcttcggc ccggtcaggg gtccggcccc acacccgcag ctcctcgagg
     4741 tcgagctctt gcgccagcgc acgcgcctgc cactcgctct ggcgtccggt gccgagcagg
     4801 gcagcggtgc gcacgccggc cggcgcgagg tgacgggccg cgagcgcagt ggccgcggct
     4861 gtacggacgc gcgtcaacgc atcgccgtcg agcgtgcaca ggaccgtccc gtcgagggcg
     4921 aagagcgcga cgacgaagct gaatcctccg ggataggtgc agtagagctt gccgccgctc
     4981 acgcccaggc tcggaacgac cgcggccatg gcgctcgcca tcgagtgacc gacagcggcc
     5041 cggacacgtt gggtgctcgc ggcgcgggcc tggc
//
LOCUS       DATBFZ010000256         4766 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_5665294, whole genome shotgun sequence.
ACCESSION   DATBFZ010000256 DATBFZ010000000
VERSION     DATBFZ010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 4766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4766
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5665294"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="VH419_07935"
     CDS             <1..328
                     /locus_tag="VH419_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043581.1"
                     /translation="AASEPDVSGAVWKLSMRDRDLDSNVVALPPGGSIEPHVGPDLDV
                     LVHVLGGSGRLTTEADSVDLFAGALLWLPRRSRRGFTAGPNGLRYLTVHRRRQALVLE
                     PPSASR"
     gene            408..662
                     /locus_tag="VH419_07940"
     CDS             408..662
                     /locus_tag="VH419_07940"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HEY7043582.1"
                     /translation="MSHRTQITLTDEQYDRLREESTRSGLGLAELVRRAVNHSYGSAS
                     QRALMEAFDSAFGSWVERVTDGEAYVESLRRGMARRLTDE"
     gene            659..1039
                     /locus_tag="VH419_07945"
     CDS             659..1039
                     /locus_tag="VH419_07945"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEY7043583.1"
                     /translation="MTILLDTSVLIDHLRGDVAARRALVGAVDNGERLACSVLTRVEI
                     LAGMRPVEEPATRRLLDALDWIGVDNTIAEHAGLLAKRYLRSHPGVDTVDFVVAATAE
                     QHNAALWTRNVKHFPMFADLRPPY"
     gene            1172..4123
                     /locus_tag="VH419_07950"
     CDS             1172..4123
                     /locus_tag="VH419_07950"
                     /inference="COORDINATES: protein motif:HMM:NF028442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEY7043584.1"
                     /translation="MSLDRLSELIKEPPIEYRPELRWWLAEGLHTDETLRDEIDTAHR
                     LGFGGMEFLAMDEAAIDHKRYGWGSEEWVHDSQVVVEETTKRNMSVSFTSGTNWSNAN
                     LPSIDPDHPAAAKELNVVVEDLDGGAVRTGPLTTVDLGTVQNPSQLPGHRGEVRRQSL
                     VAVVAARVIESTATTAVLDVESVIDLTDRASAGALHWTAPDDGSWRVFTFWMHGTGQT
                     ASPSASINYTVNYLDPDGAAALIDYWDSVVLTPALRGQIADNPRTQMYMDSLELSTFG
                     AAGLFWGRTVSDEFRSRRGYDITPWLPFLTRTVPMMAASTTYHNEPSAEHKITVDKVR
                     FDYVRTLTDLYIENMLRPFAEFLHRNGIQLRAEISYGLPFELTRPGPEVDGIETESLE
                     FAAQIDAYRLLAGPAHLFGKQYSSETGATTRNHILDHRFYDQIIATQLAAGITKTVLH
                     GWASTSGAEGATEWPGHEGMWSQFSERFDTRQPAAEFYPLWNAAIGRYQCVLRQGKPR
                     IDVGILRTDHFTDNMSGLALIDPDGRRVPDEEAYGRRWMRDRSNHWWQDLGMQDAGWT
                     YEFFDGSLLLRDDVVYADGLVQPDGPGYQALLVYQAELDADVAAHLVGWAKQGLKILV
                     VHGTREAKLVLAGQFTTHERAAERTPGLDGRDQELADTMRELLALPTVAEVDDPAETV
                     AALRSLGLRGRAEFTHDNRSVLTHLRSDGDLLHLYAYHFLYQTGDPVEVEVALPRRGA
                     VHRIDAWSGAIRPHRGVRHDDDRTVVILRLAPGETALITLDRAAEGAPESDPAAVEPV
                     AELTEWRIVVESWDAGVPRVITEDRGLGYVTREVRPTTAISRLDAGTRELLAWKEMPA
                     IGPEVSGVAEYVATMRLDAEPATGARCLLDLGSTSGGLGAVWVNDGDARGFDTSRPVV
                     DVTDDLQPGDNTVRVRVASSLNNRLLARGYYDRVPDIAADLFGLEQRMQQTEPHDHGL
                     LGPVRLMREVP"
     gene            4166..4633
                     /locus_tag="VH419_07955"
     CDS             4166..4633
                     /locus_tag="VH419_07955"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEY7043585.1"
                     /translation="MRLTEEMRRAVAEEQLGFVATVRPDGTPALSPKGTTSVWRDDQL
                     VFLHIHSEGTVANLATNPAIEINVVDPIARRGWRFRGRATVYASGAMFDEVIAHFAAQ
                     GRASAALAKAVVIMDIENADLLLSPAYDDGSTEEQMLARWLPRWRARLDRLTT"
BASE COUNT          812 a         1599 c         1599 g          756 t
ORIGIN      
        1 ggccgcgagc gaacccgacg tgtccggcgc ggtctggaag ctgtccatgc gcgatcggga
       61 cctcgactcg aacgtcgtcg cgctgccgcc cgggggatcg atcgagccgc acgtggggcc
      121 tgatctcgac gtgctggtcc acgtcctcgg cggctccgga cggctgacga ccgaggccga
      181 ctccgtcgac ctcttcgccg gcgccctgct ctggctgccg cgccgctccc ggcgcggctt
      241 caccgccggg cccaacggcc tgcgctatct gacggtccat cggcgccgac aggccttggt
      301 gctggagccg ccgtcagcca gccgctgatg ctcgaggctg cggaggcccc atgagatgcc
      361 acgatcgacc agccagtcct tgccagaagg tgatgtaagc ttacatcatg agccaccgga
      421 ctcagatcac cttgacggat gagcagtacg accgcctacg cgaagagtcg acgcgcagcg
      481 ggctcggact cgctgagcta gtccgacggg ccgtcaacca ctcatacggt tcagccagcc
      541 aacgagcgct catggaggcc tttgacagtg ccttcggcag ctgggtcgaa cgcgtcacag
      601 acggagaggc atacgtcgag agccttcgac gcggcatggc acgacggctg accgacgagt
      661 gactatcctc cttgacacct ccgtgctgat tgaccacctg cgcggcgatg tcgcagccag
      721 acgagcgctc gtcggagcgg tcgacaacgg tgaacggctg gcctgctcgg tgctgaccag
      781 ggttgagatt ctggccggga tgcggcctgt ggaggaacca gccacgcgcc gcctgctcga
      841 tgcgctcgac tggatcggcg tcgacaacac aatcgccgaa cacgctggat tgttggcgaa
      901 gcgctacctg cgctcccacc cgggcgtcga caccgtcgac ttcgtcgtcg ccgcaaccgc
      961 cgagcaacac aatgccgcgc tctggacgcg caacgtgaag cactttccca tgttcgccga
     1021 cctccgaccg ccctactagg actaccaggc ggattgaccg gacttcgtcg gcgtggttgg
     1081 atcgtttcat atccaggcgg ctgcctgtgc tcttcgcgtc tcaggtctcg tccggcccgg
     1141 acgccgttcc gcttgtccga aggagaaccc aatgtcgctg gacagactgt ccgagctgat
     1201 caaggaacca ccgatcgagt accggcccga gttgcgctgg tggctggccg agggactgca
     1261 caccgacgag accctgcgag acgagatcga caccgcgcac cggctcggtt tcggcggcat
     1321 ggagttcttg gcgatggacg aggccgccat cgaccacaag cggtacggct ggggctccga
     1381 ggaatgggtg cacgactcgc aggtggttgt cgaggagacg acgaagcgga acatgtccgt
     1441 gagcttcact tccggcacca actggtcgaa cgccaacctc ccgtcgatcg accctgatca
     1501 tcccgcggcc gcgaaggagt tgaacgtcgt cgtcgaggat ctggacggcg gagcagtgcg
     1561 aaccggaccg ctgaccaccg tcgacctcgg cactgtgcag aacccgtctc agctcccggg
     1621 gcatcgcggt gaggttcgcc gacagtccct ggtcgcggtc gtcgccgccc gagtgatcga
     1681 gtcgactgcc acgactgccg tgctcgacgt ggagtcggtg atcgacctga cggatcgcgc
     1741 atccgcgggg gcgctgcact ggacggcacc ggacgacggc agctggcggg tgttcacctt
     1801 ctggatgcac ggcacgggac agaccgcgtc accgtcggca tcgatcaact acacggtcaa
     1861 ctacctcgac ccggacggcg cggcagcgct gatcgactac tgggactcgg tcgtgctcac
     1921 tcctgccctg cgggggcaga tcgcggacaa ccccagaacc cagatgtaca tggactcctt
     1981 ggagctgtcg acgttcggcg ccgctggact gttctgggga cgtacggtgt ccgacgagtt
     2041 ccgctcccgc cgcggctacg acatcacacc ctggttgcca ttcctgaccc ggaccgttcc
     2101 gatgatggcc gcgtcgacga cctaccacaa cgaaccgtct gccgagcaca agatcaccgt
     2161 ggacaaggtc cggttcgact acgtgcgtac cctgaccgat ctctacatcg agaacatgct
     2221 gcgtccgttc gcggaattcc tgcacaggaa cgggatccag ctccgtgcgg agatcagcta
     2281 cgggctgccg ttcgagctca cccggcccgg gcccgaggtg gacggcatcg agaccgagtc
     2341 gctggagttc gccgcgcaga tcgacgccta ccggctgctg gccgggccgg cccacctgtt
     2401 cggcaagcag tactcctccg agaccggcgc gacgacccgc aaccacatcc tcgaccaccg
     2461 tttctacgat cagatcatcg ccacccagct ggcggccggc atcaccaaga cggtgctgca
     2521 cggctgggca agcacctccg gggcggaggg cgccaccgaa tggccaggcc atgagggcat
     2581 gtggtcgcag ttctccgagc ggttcgacac gcgtcagccg gccgccgagt tctacccgct
     2641 gtggaacgcc gcgatcgggc gctaccagtg cgtcctgcgg caagggaagc cgagaatcga
     2701 cgtcggcatc ctgcggaccg accacttcac cgacaacatg tccgggcttg ccctgatcga
     2761 cccggacggt cggcgggtgc cggacgagga ggcgtacggc cggcgctgga tgcgcgatcg
     2821 ttcgaaccac tggtggcagg acctgggcat gcaggacgcg gggtggacgt acgagttctt
     2881 cgacggatca ctgctgctgc gggacgacgt ggtgtacgcc gacgggctgg ttcagcccga
     2941 cgggcccggt tatcaggcgc tgctggtcta ccaggctgag ctcgacgcgg acgtggcggc
     3001 gcatctggtc ggctgggcga agcagggtct gaagatcctc gtggtgcacg gcactcgcga
     3061 agcgaagctg gtgctcgcgg gccagttcac gacacacgag cgcgccgccg agcgcacgcc
     3121 cggcctggac ggtcgtgatc aagagctcgc ggacacgatg cgcgagctgc tcgcgctgcc
     3181 gaccgtcgcc gaggtcgatg atccggctga gaccgtcgca gccctgcggt cgctcggtct
     3241 gcggggtcgc gcggagttca cccacgacaa tcgcagtgtc ctcacgcacc ttcgttccga
     3301 cggagatctg ttgcatctgt acgcgtacca cttcctttac cagacaggtg atccggtcga
     3361 ggtggaggtg gcgctgccca ggcgtggcgc ggtgcatcgg atcgacgcgt ggagcggtgc
     3421 gatccggccg caccgcggcg tgcggcacga cgacgaccgc accgtcgtga tcttgcggct
     3481 ggcacccggg gagacggcgc tgatcacgct ggatcgcgcg gccgaaggtg ccccggaatc
     3541 cgacccagcg gcggtcgagc cggtggccga gctcaccgag tggcggatcg tcgtcgagag
     3601 ctgggacgcc ggcgtgccgc gggtgatcac cgaggatcgc gggctgggct acgtcacgcg
     3661 cgaggtccgg ccgaccacgg cgatcagccg gctagacgcc ggaacccggg agctgcttgc
     3721 gtggaaggag atgccggcga tcgggccgga ggtgtcgggc gtcgccgagt acgtcgcgac
     3781 gatgcggctg gatgcagagc cggcaaccgg agctcgctgt ctgctggacc tcggctcgac
     3841 gtccgggggg ctgggcgcgg tgtgggtcaa cgacggcgac gctcgaggct tcgacacatc
     3901 ccgacccgtc gtcgacgtca ccgacgatct gcaaccggga gacaacacgg tgcgggtccg
     3961 ggtggcgagc tccctcaaca accggctgct cgcccgcggc tactacgacc gcgtgcccga
     4021 catcgccgcg gacctgttcg gactcgagca acggatgcag cagacggagc cgcatgatca
     4081 tggactgctc gggcccgtcc ggctgatgcg agaggttcca tagcccccgc ggatcccgtc
     4141 gacgcatcga cgcacaatac gacctatgcg actgaccgag gagatgaggc gagccgttgc
     4201 cgaggagcaa ctcggcttcg tcgcgacggt ccgaccggac gggacgccgg ccctgtcacc
     4261 gaagggcacg acgagcgtct ggcgggatga tcaactggtc ttcctccaca tccactccga
     4321 aggaacggtt gccaacctgg cgaccaaccc ggcgatcgag atcaacgtgg tcgacccgat
     4381 cgcccggcga ggctggcggt ttcgcggtcg tgccaccgtg tacgcgtcag gcgcgatgtt
     4441 cgacgaggtg atcgctcact tcgcggcgca gggccgcgcg tcagccgcgc tcgccaaggc
     4501 ggtcgtgatc atggacatcg agaacgcgga cctgctgctc tcccccgcgt acgacgacgg
     4561 ctccaccgag gaacaaatgc tggctcgatg gctgccccgc tggcgggccc gcctcgaccg
     4621 cctgacgacc tgagccccac gaactcgccc cgcgattgtt ccaagctcca cgtctacgcg
     4681 tcctcgatgc cacgatcgac agcctgggtc agacctgcga ggtggttgtc gaggatgtcg
     4741 cgaatcagtt cagcgttgac ccgaaa
//
LOCUS       DATBFZ010000257         2729 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_2142288, whole genome shotgun sequence.
ACCESSION   DATBFZ010000257 DATBFZ010000000
VERSION     DATBFZ010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2142288"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2270)
                     /locus_tag="VH419_07960"
     CDS             complement(<1..2270)
                     /locus_tag="VH419_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HEY7043586.1"
                     /translation="MLRRSSSAHLEGTPRQVTRHRRRDSRVRLRFFGGTGRRRIVAVG
                     VAIVVLAGAGLAYADTTVFGQNQVGTEYPNGIQVSDDQIIEPLGDRLMTPYGKFMSST
                     VSPNGRFLAATSTDKSVVLQIFDLSSYQLIWRVGTATGVNQRFTDLTVGQEGPTYSPD
                     GTVLWLPEQTGLARFPVNADGTLGAPTTVSIPTVSGKAALVGQSVYSPDGSTLYSAVN
                     GQNTVVALDPITGAIKQTWNVGIAPRELKFVGSTLYVSNEGGRQAQAGDTTMGSYGTQ
                     VPANGYLGTSTTGTVSVIDTANAAAAVGSIPVGLHPTAMYVRGHALFVANAFDDTVSV
                     IDTTKNQVVQTIETRPWPSSDVGYSPTSIAVTSDDHLLVTLGRANAVAVYQYKGDPQE
                     PVSYIGLVPTDYYPATIATVGGQIFVTNTRGIDARGPNLSFNKGFGTTVAVGHGTHST
                     TGSLTRFTLPTDKQIRKSYTDTVFAQNGWGKKDVRQKEGEKAKALPVPERIGNPSPIK
                     YVWLLVKENRTYDQVYGDLPQGNGDPTLTQFGNRATPNQHALAKQFGLYDNVYDVGTN
                     SAEGHNWLMQGDDPEYTESSAGEYQRSYDTEEDVLGHQRSGFLWTAVEDAGKTARNFG
                     EFEYMEGKPSGTWQDYYCAVKSVRSGGDPSLLTAPNLKGNYGSVIPSLNAITNHLSPP
                     FDEAIPDIYRYQIWKQEFEASGPANFNMMWLSSDHTGGPTTPRSAVADGDLAVGQIVD
                     TISHSAYWKDSAIFVV"
     gene            complement(2591..2663)
                     /locus_tag="VH419_07965"
     tRNA            complement(2591..2663)
                     /locus_tag="VH419_07965"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2628..2630),aa:Ala,seq:cgc)
BASE COUNT          388 a          877 c          954 g          510 t
ORIGIN      
        1 tcgaccacga agatcgcgga gtccttccag taggcgctgt gcgagatggt gtcgacgatc
       61 tggcccacgg cgagatcacc gtcggcgacc gcggagcgcg gcgtcgtcgg accgccggtg
      121 tggtcgctgg agagccacat catgttgaag ttggccgggc cgctggcctc gaactcctgc
      181 ttccagatct gatagcggta gatgtccggg atcgcctcgt cgaacggcgg cgacaggtgg
      241 ttggtgatgg cgttcagcga cgggatcacc gagccgtagt tgcccttcag gttgggggcg
      301 gtcaggagcg acgggtcacc gccgcttcgg acgctcttca cggcgcagta gtagtcctgc
      361 cacgtaccgg acggcttgcc ctccatgtac tcgaactccc cgaagttccg cgcggtcttg
      421 ccggcgtcct cgactgccgt ccagaggaag cccgaacgct ggtggcccag gacgtcctcc
      481 tcggtgtcgt agctgcgctg atattcgccc gcggacgact cggtgtactc ggggtcgtcg
      541 ccctgcatca gccagttgtg gccctcggcc gagttcgtgc cgacgtcgta cacgttgtcg
      601 tagaggccga actgctttgc aagcgcgtgc tggttcggtg tcgccctgtt gccgaactgc
      661 gtcagggtcg gatcgccgtt gccctggggc aggtcgccgt acacctggtc gtaggtgcgg
      721 ttctccttga ccagcagcca gacgtacttg atcggcgagg ggttcccgat gcgctccggg
      781 accggtagcg ccttggcctt ctcaccctcc ttctgccgca cgtccttctt gccccagccg
      841 ttctgggcga agaccgtgtc ggtgtacgac ttgcggatct gcttgtccgt cggaagcgtg
      901 aagcgggtca gcgagccggt ggtgctgtgc gtgccgtgac ccaccgcgac cgtggtgccg
      961 aagcctttgt tgaacgagag gttgggtccg cgcgcgtcga taccgcgggt gttggtgacg
     1021 aagatctggc caccgaccgt cgcgatggtc gccgggtaat agtccgtcgg caccaggccg
     1081 atgtagctca ccggctcctg cgggtcgcct ttgtactggt agacggccac ggcgttcgcg
     1141 cggccgaggg tgaccagcag gtggtcgtcg ctcgtcacgg cgatgctcgt gggcgagtag
     1201 ccgacgtccg acgagggcca cggcctggtc tcgatcgtct gcacgacctg gttcttggtc
     1261 gtgtcgatga ccgagaccgt gtcgtcgaag gcattggcga cgaacagcgc gtgccccctg
     1321 acgtacatcg cggtcgggtg caggccgacg gggatcgatc cgaccgccgc cgccgcgttc
     1381 gccgtgtcga tgacgctgac ggttccggtc gtcgacgtgc cgaggtaccc gttggccggc
     1441 acctgcgtgc cgtatgagcc catggtcgtg tctccggcct gcgcctggcg gccgccctcg
     1501 ttgctcacgt aaagcgtgct gcccacgaac ttgagctcac gaggcgcgat gccgacgttc
     1561 caggtctgct tgatcgcccc ggtgattgga tcgagcgcca cgacggtgtt ctgcccgttg
     1621 accgccgaat agagggtcga cccgtcgggc gagtagacgc tctggcccac cagggcggcc
     1681 ttgccgctca ccgtcgggat cgagacggtc gtcggcgcac cgagcgtgcc gtccgcgttg
     1741 accgggaacc gcgccagacc cgtttgctcc gggagccaga ggaccgtccc gtcgggcgag
     1801 tacgtcgggc cttcctggcc gacggtgagg tcggtgaacc gctggttgac cccggtcgcg
     1861 gtgccgaccc tccagatcag ctggtagctc gacaggtcga agatctgcag gacgaccgac
     1921 ttgtcggtgc tggtggccgc gaggaagcgt ccgttcgggc tgaccgtcga ggacatgaac
     1981 ttcccgtacg gagtcatcag gcggtcgccg agcggctcga tgatctggtc gtcggagacc
     2041 tggatcccgt tgggatactc ggtgccgacc tggttctggc cgaacacggt tgtgtcggca
     2101 taggccagtc ccgcgccggc gaggacgacg attgcgaccc cgaccgcgac gatgcggcgc
     2161 cggccggtcc cgccgaagaa ccggagacgg actctcgagt ctcgacggcg atgccgtgtg
     2221 acttgccggg gtgtcccttc gaggtgcgca ctgctggacc gtctgagcat cactccccct
     2281 ttgcgtgagc ggaaggagcg gacggcgcac ggcacttcga cgccgtggcg tcgacgacga
     2341 ccggcggtcg tcgcctcgat gaaggtgggt gcgtcatctc actccctcgg cttgatctga
     2401 cgacggcgcc accgtaagcg cgacctacgc ttcgacgggg ttacgctggt gtgtgaatgc
     2461 ggtgacagct gcgccgtgcc cgagccgcca tcgctggtcg gcgctgtatc agctgctcgg
     2521 acgaacggag ggctccggcc tctggactgc ctccacccgg aagcggtgtc tgacctcggg
     2581 ccggtgacga tggagctagc cggactcgaa ccggcgacct cctgggtgcg atccaggcgc
     2641 tctcccaact gagctatagc cccgcgcccg cacagtgtag ccgcgcctaa gtgcgggctc
     2701 tagaacgcgc gggccgcggc gatgacggg
//
LOCUS       DATBFZ010000258         2702 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_2143574, whole genome shotgun sequence.
ACCESSION   DATBFZ010000258 DATBFZ010000000
VERSION     DATBFZ010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2702
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2143574"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..613
                     /locus_tag="VH419_07970"
     CDS             <1..613
                     /locus_tag="VH419_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043587.1"
                     /translation="RVASVIGVPMTALTQRQRWDNPMIGPEAVEVLRRVNERLGDRLN
                     QRQYERVIKGTLVPLLAAHGESRFDVPPEEIPWLAERADAFIEDLVARGYPVVGDLEE
                     LRPVRREGGRRPDSATEAATLAAAVDSLALLAEAYANAWWQHRRGDIEGAAESRNIRS
                     LMRQVTFSGQRKVLRLADRNALVAKAVGSTVERRRRRRGEPRG"
     gene            complement(603..1574)
                     /locus_tag="VH419_07975"
     CDS             complement(603..1574)
                     /locus_tag="VH419_07975"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HEY7043588.1"
                     /translation="MHAAVLHTPGEPVTYADHPDPVQAAGQTLIRVKAAPVVPLDLLC
                     ASGTSYFGRPQVPYVPGVQGVGVVAASESIPGGSRVFFATEAGKQPGDGGMAELCAVP
                     DGDVIPVTADVDDAALAALGLSAVAAWMILTWRARLREGERVLVLGAGGAVGQAAIGA
                     ARLLGARRVVAVCRSSAADQRAREAGADEVVRLTDDVDRLTEQFRDAVDGAVDVVVDP
                     VFGVAATAASRVLANGGRLVNLGGASGDTAPFSSSVLRSRSAGVLGYTNDALSVEQRR
                     EAFDAVVSHAAAGRMHVAHETWPLADAENAWTLQASGRASGRLVLIP"
     gene            complement(1591..1950)
                     /locus_tag="VH419_07980"
     CDS             complement(1591..1950)
                     /locus_tag="VH419_07980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043589.1"
                     /translation="MSKYLLDKFLFTVDRDPSLVERYREDPDGTVAWWEDEMANKLLN
                     CVDEEKSTWLAFTDEERRALRAHDHVALFGMGAHPFLTLTLFIAMFERDHGPLEYQRA
                     YGRALQHLTLPYPDIAT"
     gene            complement(1947..>2702)
                     /locus_tag="VH419_07985"
     CDS             complement(1947..>2702)
                     /locus_tag="VH419_07985"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043590.1"
                     /translation="KIETFRETLTRAEPDVLVMVGSDHFHQLWLDNMPQFLVGKAPCY
                     DANWYNEEREFGLPRFRLAGDEELSQHILRGGLDRSFDLAFSNELRIDHSITCPIITL
                     RPQADLPIVPVYTNIFAPPLPQPKRFVQLGRTIRELVESWPSDKRVAIIGTGHLSLEL
                     GGPRQFGDHGPDPEFDRKAVDWIASGDLEGCLSEVTLDSLHTPGNATHGFMDFMLMMG
                     VAGEGAKADHVDTLDLFHTMEAYFTWYPNGVPA"
BASE COUNT          437 a          917 c          939 g          409 t
ORIGIN      
        1 gcgggtcgca tctgtcatcg gagtacccat gaccgcgctg acacaacggc aacggtggga
       61 caacccgatg atcggcccgg aggccgtgga agtactccgg cgcgtcaacg agcggctcgg
      121 cgaccggctc aaccaacgtc agtacgagcg cgtcatcaaa gggacgctcg tgccgttgct
      181 cgcagcgcat ggggagtcgc gcttcgacgt tccccccgag gagatcccct ggcttgctga
      241 gcgcgccgac gcgttcatcg aagacctcgt tgctcgcggc tatccggtcg tcggagacct
      301 cgaggagctc cggcctgtac gccgcgaggg aggccgacgg ccggacagcg cgaccgaggc
      361 cgccaccctc gccgcggccg tcgactcact ggcgctcctc gccgaggcgt acgcgaacgc
      421 ctggtggcaa caccggcgag gtgacatcga aggggcggcg gaatctcgaa acatccgcag
      481 cctgatgcgc caggtcacgt tctccggcca acgcaaggtg ctgcggctgg ccgaccggaa
      541 cgcgctggtt gccaaggcag tcggctcaac ggtcgagcga cgccgccggc ggcgcggtga
      601 acctagggga tgaggaccag acggccggag gcccggccgg acgcctgcag ggtccaggcg
      661 ttctccgcat cggccagtgg ccaggtctcg tgtgcgacgt gcatccgacc ggcggcggca
      721 tggctgacca cggcatcgaa ggcttcccgg cgttgttcga ccgacagcgc gtcgttcgtg
      781 tagccgagta cgccggctga gcggctccgc aggaccgagg acgagaacgg cgcggtgtca
      841 ccggacgcgc ctccaaggtt caccagccgt ccgccgttgg cgagcacgcg tgacgcagcg
      901 gtggcagcga cgccgaagac cggatcgacg accacgtcga ccgcaccgtc gacggcgtcc
      961 cggaactgct cggtgagccg gtcgacgtcg tcggtcagtc gtacgacctc gtcggctccg
     1021 gcctcacgag cccgctgatc ggcggccgag gagcggcaca ccgcgacgac ccgacgtgct
     1081 cccagcagcc gggccgcgcc gatcgccgcc tggcccactg cacctccagc gccgaggacg
     1141 agaacgcgtt cgccttcacg cagccgcgcc cgccaggtga ggatcatcca ggccgcgacc
     1201 gccgagaggc cgagcgcggc cagggcagcg tcgtcgacgt ctgccgtcac tgggatgacg
     1261 tcgccgtccg ggacggcgca cagctccgcc atgccaccgt cccccggctg cttgcctgct
     1321 tcggtggcga agaacacccg acttccgcct ggaatggact cggacgccgc cacgacgccc
     1381 acaccctgga cgccggggac gtacgggacc tgcggacgac cgaagtacga cgtcccggat
     1441 gcacagagca ggtccagtgg gacgaccggt gcggccttca cgcggatcag ggtctgcccg
     1501 gccgcctgta ccggatccgg gtgatcggca tacgtcaccg gctcgcccgg agtgtggagc
     1561 acggcagcat gcatcgacgt cctcctggcc tcaggtggcg atgtcgggat aggggagtgt
     1621 caggtgttgc aacgccctgc cataggccct ctgatactcg agcggaccgt ggtcgcgttc
     1681 gaacatcgcg atgaacaacg tgagcgtgag gaacgggtgc gcgcccatcc cgaacaacgc
     1741 gacgtggtcg tgcgctcgca gcgcacgccg ttcctcgtcg gtgaaggcga gccacgtcga
     1801 cttctcctcg tcgacgcagt tgagcagctt gttcgccatc tcgtcttccc accacgcgac
     1861 ggtgccgtcg gggtcctccc ggtagcgctc gaccagcgac gggtcgcggt ccacggtgaa
     1921 gaggaacttg tcgagcaggt acttgctcat gccggtactc cgttcggata ccaggtgaag
     1981 tacgcctcca tcgtgtggaa gaggtccagc gtgtcgacgt ggtccgcctt ggcgccctcg
     2041 cccgcgacgc ccatcatcag catgaagtcc atgaagccgt gggttgcgtt gccgggcgtg
     2101 tgcaggctgt cgagcgtgac ctcggacagg cagccctcga ggtcgccgct cgcgatccag
     2161 tcgaccgcct tccggtcgaa ctccggatcc ggcccgtggt cgccgaactg acgggggccg
     2221 ccgagctcga gggagagatg tccggtcccg atgatcgcga cccgcttgtc cgatggccag
     2281 gactcgacca gctcacggat ggtccggccg agctgcacga accgcttcgg ttgcggcagg
     2341 ggcggcgcga agatgttcgt gtagacgggc acgatcggca gatccgcctg cgggcgcagc
     2401 gtgatgatcg ggcaggtgat gctgtggtcg atccgcagct cgttgctgaa cgcgaggtcg
     2461 aagctgcggt cgagaccgcc gcgcaggatg tgctgcgaga gctcctcgtc acccgcgagc
     2521 cggaagcgcg ggaggccgaa ctcgcgctcc tcgttgtacc agttggcgtc gtagcacggc
     2581 gccttgccga ccaggaactg cggcatgttg tccagccaca gctggtggaa gtggtcgctg
     2641 ccgaccatga cgagcacgtc gggctcggct cgggtcagcg tctcgcggaa cgtctcgatc
     2701 tt
//
LOCUS       DATBFZ010000259         2843 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_2796281, whole genome shotgun sequence.
ACCESSION   DATBFZ010000259 DATBFZ010000000
VERSION     DATBFZ010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2843
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2796281"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="VH419_07990"
     CDS             <1..573
                     /locus_tag="VH419_07990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043591.1"
                     /translation="PTGLFNAAPTTRVAGQLAFPSDHTGVEATLMCETSTAQRQRAAT
                     ATVTSAATTSSPTTIPTTTAAGSSGPSAETVAAITAAFRDVFDGSVNDVDVKLSALED
                     ADVLRPYFLATYGATKAVASRIRVRIDQIKLADPTHADVTYTLLLDGSAVLDHLPGHA
                     VRIGDRWLVSRRTYCDVSTQGATTIPPVCR"
     gene            complement(607..1212)
                     /locus_tag="VH419_07995"
     CDS             complement(607..1212)
                     /locus_tag="VH419_07995"
                     /inference="COORDINATES: protein motif:HMM:NF018446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HEY7043592.1"
                     /translation="MLTKTRLEAFSDGVFAIAITLLVIEIRPPTLAEGETLAHGLWQQ
                     WPSYLGYLLSFLVLGVMWLNHHRIFEPARRVDGTVLVLNLNLLLWAVLIPFPTAVVAD
                     FLRDGGTDAKTAVALYGGVVLLAAIAFTALFIGITRPSIVDELPPPEVVRAARVRFGI
                     GVGFYGFAFALSWLSPALALAVHGTMAAYYLTAQSSRLNAG"
     gene            1397..2788
                     /gene="zwf"
                     /locus_tag="VH419_08000"
     CDS             1397..2788
                     /gene="zwf"
                     /locus_tag="VH419_08000"
                     /inference="COORDINATES: protein motif:HMM:NF009492.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEY7043593.1"
                     /translation="MSEPSSCDVFVIFGITGDLAKVMTFRSLYRLEQRKLLDCPIVGV
                     AVDDWTVDQLVERARTSIVGTGEQLDEDVFARLAARFSYVAGDFADAATYGRVANAIT
                     GAERPVFYLEIPPFLFGTVVKGLAGADLAKNARVVVEKPFGHDLASARALADELHQYL
                     DESQLFRIDHYLGKMGLEEILYLRFGNTMLEPVWNRNFIQSVQITMAEDFGVEDRGHF
                     YDPVGALRDVVVNHLMQVVAAAAMEAPAGGDPTTLKDAQTAVFRAVETADPAHYVRGQ
                     YDGYQAINGVAADSTTETYAALRLNIENWRWSGVPFFIRTGKRLPVTQTELRLVFKMP
                     PRLGFGLRGADSQPDQVVIRLDPSTGIRIELEARHAEARAPLPIELDMLFAEQGGEGA
                     TPYEVLLHAAMVGQNVRFTRQDGVEEQWRIMQPLIDSPPPVHPYAPGSWGPDAAESLV
                     AGFGRWHTPWGAS"
BASE COUNT          492 a          974 c          940 g          437 t
ORIGIN      
        1 ccgaccggac tgttcaacgc ggcaccgacc acccgtgtcg ccggtcagct cgcgtttccc
       61 tcggaccaca ccggcgtgga agcgacgctg atgtgtgaga cgtcgacagc ccagcgccaa
      121 cgtgccgcga ccgcgacggt gacgagcgcg gcgacgacct cgagtccgac caccatcccc
      181 acgacgacgg cagcagggtc gagcgggccg agcgccgaga cggttgcagc gatcacggcc
      241 gccttccggg acgtcttcga tggaagcgtc aacgacgtcg acgtcaagct ttctgcgctc
      301 gaggacgctg acgtgttgcg gccctacttc ctcgccacct acggagccac gaaagccgtc
      361 gcgagccgca tccgtgtgcg catcgaccag atcaagctcg ccgaccccac gcacgccgac
      421 gtcacgtaca cgctgctcct cgacggctcg gccgtcctcg accaccttcc cggccacgcc
      481 gtacggatcg gcgaccgctg gctggtcagc cggcgcacgt attgcgacgt cagcacgcaa
      541 ggcgcgacca ccatcccgcc ggtctgccgg tgacgagcgc gcaggaccct cgtccccggt
      601 tcactcctac ccggcgttga gccgcgacga ctgcgcggtc aggtagtacg cggccatcgt
      661 gccgtgcacg gcgagcgcca acgccggcga cagccacgaa agcgcgaatg cgaacccgta
      721 gaacccgacg ccgatgccga acctcactcg agcggcgcgg acgacctcag gcggcgggag
      781 ctcgtcgacg atgctcggtc gcgtgatccc gatgaacagc gcggtgaacg cgatcgcggc
      841 gagcaagacg acaccgccgt agagggcgac cgccgtcttc gcatcggtgc cgccgtcccg
      901 aaggaagtcg gcgacgactg ccgtcgggaa cgggatgaga accgcccaca gcagcaggtt
      961 gaggttgagc accaacacgg tgccgtcgac gcgacgtgcc ggctcgaaga tgcgatggtg
     1021 gttcagccac atcacgccga ggaccaggaa gctcagcagg tagccgagat agctcggcca
     1081 ttgctgccac aacccatgcg cgagcgtctc gccttcggcg agcgtcggag ggcggatctc
     1141 gatcaccaac agtgtgatgg cgatggcgaa cacgccatcg gagaatgcct cgagtcgggt
     1201 cttcgtgagc attccgcagt ctgcgtcgcc gtcaggcctg cctcgtcacg cagctcgacc
     1261 ggacgcctgg ctggtaccga cacagccgac acctgccgga gcagggtgct gggcgtgagc
     1321 gcggaccgtg cgagtcttgg gccgccacac agacgagtgc cgggacaacg acgctgagga
     1381 ccggggtgac gggcaaatga gtgaaccttc cagctgtgac gtcttcgtga tcttcggcat
     1441 caccggtgac ctggccaagg tgatgacgtt ccggtccctg taccggctcg agcagcgcaa
     1501 gctgctcgac tgcccgatcg tcggtgtggc ggtcgacgac tggacggtcg atcaactcgt
     1561 cgagcgggcc cgcacgtcga tcgtcgggac tggtgagcaa ctcgacgagg acgtgttcgc
     1621 tcggctcgcg gcgcgcttct cgtatgtggc cggcgacttc gccgacgcgg ccacctacgg
     1681 gcgagtggcc aacgcgatca cgggcgcgga gcggccggtc ttctacctcg agataccgcc
     1741 gttcctgttc ggcacggtgg tgaagggtct ggctggggcc gacctcgcga agaacgcccg
     1801 ggtcgtcgtc gagaagccgt tcggacacga cctcgcgtcc gcgagagctc tggccgacga
     1861 gctccatcaa tacctcgacg agtcgcagtt gttccggatc gatcactacc tcgggaagat
     1921 ggggctggag gagatcctct acctgcggtt cggcaacacg atgctggaac cggtctggaa
     1981 ccggaacttc atccagagcg tgcagatcac gatggccgag gacttcggtg tcgaagaccg
     2041 tgggcacttc tacgaccctg tcggcgcgct gcgtgatgtg gtcgtgaacc acttgatgca
     2101 agtcgtggcg gcggcggcga tggaggcgcc cgcgggcggc gatcccacca ccctcaagga
     2161 cgcgcagacc gccgtcttcc gagcggtcga aacggccgac cctgcccatt acgtgcgggg
     2221 ccagtacgac gggtaccagg cgatcaacgg cgtcgccgcc gactccacca ccgagaccta
     2281 tgccgcgctc cggctgaaca tcgagaactg gcgctggtct ggcgtgccct tcttcattcg
     2341 caccggcaag cggctccccg tgacccagac cgagctccgt ctcgtgttca agatgccgcc
     2401 acggctcggc ttcggcttgc gcggcgccga ctcccagccc gatcaggtcg tcatccgact
     2461 cgacccgtcg accggcatcc gcatcgaatt ggaggcgcgc cacgccgagg cgcgggcgcc
     2521 actgccgatc gagctcgaca tgttgttcgc agaacaaggt ggtgagggcg ccacgcccta
     2581 cgaggtgctc ctgcacgcag cgatggtcgg tcagaatgtg cgcttcacac ggcaagacgg
     2641 cgtggaggag cagtggcgaa tcatgcagcc gctcatcgac tcgccgcctc ccgtgcatcc
     2701 gtacgcaccc ggctcatggg gaccggacgc ggccgagtcc ctcgtcgccg gtttcggccg
     2761 ctggcacacg ccgtggggcg cgtcgtgacc ctggcgccct agaggatgct cgcatggcga
     2821 cctttgtcct ggttcacggc gcc
//
LOCUS       DATBFZ010000260        10393 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_831644, whole genome shotgun sequence.
ACCESSION   DATBFZ010000260 DATBFZ010000000
VERSION     DATBFZ010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 10393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10393
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_831644"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="VH419_08005"
     CDS             <1..193
                     /locus_tag="VH419_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013861441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043594.1"
                     /translation="PDVPIGEALLDQRNLAGIGNLYKSEVLFLLGVHPDMPAGEASGV
                     DNPSQARVSYWCPSCQPEP"
     gene            complement(312..635)
                     /locus_tag="VH419_08010"
     CDS             complement(312..635)
                     /locus_tag="VH419_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006549414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY7043595.1"
                     /translation="MILVRRLLGDVLRELRREQGRTLREVSSDARVSLGYLSEIERGQ
                     KEASSECLAAITRALDVPLSVIMAKVSGEIAREEAVSALTSIHVRTPDAESIRVRADV
                     TNVAA"
     gene            complement(770..1273)
                     /locus_tag="VH419_08015"
     CDS             complement(770..1273)
                     /locus_tag="VH419_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidohydrolase family
                     protein"
                     /protein_id="HEY7043596.1"
                     /translation="MTDPGSEVAEACLRALHHRSATLATAESLTAGLVVSTLATIPGA
                     SDVLRGGLAAYATEVKVDVLGVDSEVIARVGVISAECAEQMALAARDLFRADWAVATT
                     GVAGPDSQDGHPPGEVYVAVAGPENLVSSQRLMLTGDRQQIRSASVDGVLELLLTALE
                     HTDRRGP"
     gene            complement(1281..1952)
                     /gene="pgsA"
                     /locus_tag="VH419_08020"
     CDS             complement(1281..1952)
                     /gene="pgsA"
                     /locus_tag="VH419_08020"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007077522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEY7043597.1"
                     /translation="MADDRYSDPAAGGSGATPSADLDPVSNWNLPNALTTLRILLVPV
                     FGWLLLSDGGEDTGLRVWAFVVFVVAILTDKIDGELARRRNLITDFGKLMDPIADKAL
                     TGMAFIGLSIIGELWWWVTILVLGREVGITLMRFWVIRYGVMPASRGGKIKTTLQAVA
                     LSAFILPLRDLDGAWASVGDVLWWLAVIVMAAAVVVTLVTGADYVRDAIRLRREGKAA
                     ERAAS"
     gene            complement(1962..3410)
                     /locus_tag="VH419_08025"
     CDS             complement(1962..3410)
                     /locus_tag="VH419_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY7043598.1"
                     /translation="MQPGSATTVALVTLGCARNDVDSEELAARLELGGFRLVEEASDA
                     ETVVVNTCGFVEAAKKDSIDALLAVADLKEAGTVRAVVAVGCLAERYGEQLATSLPEA
                     DAVLGFDDYPDISARLRHILDGHRHGAHTPHDRRKLLPVTPIERHAAVAPSVPGHAQS
                     PPDLPAGVGPASGPRAIRRRLGSGPMAPLKLASGCDRRCSFCAIPSFRGAFVSRSPED
                     VVAEGRWLASEGVRELFLVSENSTSYGKDLGDLRLLETLLPDLASVDGVRRVRVSYLQ
                     PAEMRPSLVETIAATPGVASYFDLSFQHASAPVLRRMRRFGDAERFLGLVDEIRRHSP
                     SAGIRSNVIVGFPGETEADLDVLSDFLTAARLDAVGVFGYSDEDGTEAVGLDGHHDQD
                     EVRDRVEHVTRLADELTAQRAEDRRGELVDVLLERIDGTVGEGRAACQGPEVDGTTTV
                     INLSADAGVGDVVPARVTGSSGVDLVADARTQ"
     gene            complement(3593..5482)
                     /locus_tag="VH419_08030"
     CDS             complement(3593..5482)
                     /locus_tag="VH419_08030"
                     /inference="COORDINATES: protein motif:HMM:NF024855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY7043599.1"
                     /translation="MEQQALIEPPDVDVSTGPSGEDTSSDDGLVVRRRAWLMAAVAGT
                     SFALSAGYLWRFGQTGDGLPLAVGVVLGVLAVCFGVAFGDARTPLLVADPTGLRVRLG
                     RRWIGVPWTHVECVEVDRRGRLRDGHVAVLVHGPDEEVAQAGRRARLAARTNRLFYDA
                     PLVVPFGLTTTVSVADIPGTLVALAGGRAPVVDVDETESEPEPTVALTNGPAEPADEP
                     LQASADDDGGPGRPGKPRRPLTAAREAMPSLPLAKVVAALRSHPARREEVTLPAGREP
                     ATLGTLALSEPYADTEGLPEREQLKRQADADGAELQPAETSDETLGETQPQRDQDAPV
                     SNVALIIDATTDLSARAMQRWRAGGQPTAMAGRTGDGADSGAPAAAAGDLVIGGQVRS
                     ARERVGLDVDELAERTRIRPYVIESIEVDDFTPCGGDFYARGHLRMLARVLGLDADVL
                     VATYDETFATSPINPRAVFGAELATSPTGLVRGGASGANWGGLIAAVLVLVLVWGVVK
                     YFADDPPRLTSGVAPTHNSAGLGSPGPGNRSLLPSKPPVGSVRIAAVGGASRVVVKNR
                     HGHVVWQGLLTDGQARRVHGLAPMRVHADNGGVIRLHSNGHLLGFMGEPGSSAHRIIR
                     ATHPR"
     gene            complement(5482..7974)
                     /locus_tag="VH419_08035"
     CDS             complement(5482..7974)
                     /locus_tag="VH419_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEY7043600.1"
                     /translation="MATRTSSPPRSRSKARSRSGTSGSQTRRSSSRKRSASSRSRSRS
                     SARRGGPGLIALFVSGIGRGLRAVWLGIAHVIGAIVRRIGSTARDLEPEHRRDGIGLA
                     LVGAAIVVAAAEWWQLPGSVGDVLRAVMEGAVGLAAYAVPLLLLLAAWRAMRAPELNG
                     PAGRPVIGWTAISLGVLGLIHIQHGLPRPNGGQEAMRGAGGAVGYVGSAMVADLFRST
                     VIAVPLLVLLTFFGLLVVTATPVYQIPRRLTALRDKVMGRKPADPHAVEEVPVERPEP
                     LRRTRPRSRVGAMADDEPTEPVVASRTEAGGPSQPHLPDTGDNGPLEPPPHTPLPQRV
                     EQLSLSGDITYTLPKNDVLRSGSAHKARSAASDAIVDRLTEVLEQFDIDAQVTGYTRG
                     PTVTRYEVELGTAVKVEKVTALSKNIAYAVASADVRILSPIPGKSAIGVEIPNPDAEL
                     VSLGDVLRAGSARHDHHPMVIGLGKDVEGGFVAANLTKMPHLLVAGATGSGKSSFINS
                     MICSVLMRATPDEVRMILVDPKRVELTVYDGIPHLITQIITSPKKAAEALQWVVREMD
                     LRYDDLANFGFRHVDDFNKAVRAGAVRVPPGVDRVLTPYPYLLVVVDELSDLMMVSPR
                     DVEDAIVRITQLARAAGIHLVLATQRPSVDVVTGLIKANVPSRLAFATSSLADSRVIL
                     DQPGAEKLVGKGDALFLPMGASKPGRIQGAWVTEAEIRQVVGHCKQQLQPTYRDDVTA
                     SVQSRRELDDDIGDDLDLVCQAVELVVSTQFGSTSMLQRKLRVGFAKAGRLMDILESR
                     GVVGPSEGSKARDVLVKPDDLDDVIAGLRGES"
     gene            complement(8173..9354)
                     /locus_tag="VH419_08040"
     CDS             complement(8173..9354)
                     /locus_tag="VH419_08040"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY7043601.1"
                     /translation="MQSITIRDPAKDDIERSFDVRNRSFGPLPEGAGPGWHAGVEKAI
                     DEGRTLAAYDGDLLVARALIWPFRQFWGGRVLAMAGIAGVVVAPEYRGRGVGSALMQG
                     MVERGRDLGYPVSALYPATVPVYRQNGWEIAGVQHRTSIKARLLRDLRTTAETPDVRE
                     VGPSDAEHLLAIMREQYAAGRVNGVRDHDADEFAEELAAETVFAYAMHDGFVVYGWED
                     RDIVVYQLVAGSPASARNLWSVVGSSSSIVDTVHAYLAPDDPIHQILGETVLRDVRQE
                     RWMLRVLDVEAALSGRGYPPATDTQVGLVVDDPLLPGGGVTGRLFVSGGTGTFVPDEG
                     VRSDDAAVRLGPNGLAALYAGTPTATLLTAGLVAGGTAESRDQLDSVFAGRPAYLLEY
                     F"
     gene            complement(9457..>10393)
                     /locus_tag="VH419_08045"
     CDS             complement(9457..>10393)
                     /locus_tag="VH419_08045"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HEY7043602.1"
                     /translation="DGAVLIAYLRVGDRLDALSVDAHSAELQSLGDLAEVRRVLAGMQ
                     ADLDMSATQLPQALRSTVQAGLRARLEDLADRLVAPLLEHLGDRRLVVVPSGALAGVP
                     WTLLPGLFGRPLTVPRSATAWLTTRPDGHRAASAGLVAGPRVDRAAEEVEAAAKAWGH
                     ATIVRQDRADAARVRDLASSVDVFHVAAHGRHSADNPLFSGLELADGPWFGYDIDQLD
                     AIPSTVILSACELGRSSIRWGEETIGMTVAWLHAGARTVIASPASVDDDVACYTLAAT
                     HHYLAAGRPPADALATAMVDVGGPIPAPFVCFGSG"
BASE COUNT         1601 a         3732 c         3566 g         1494 t
ORIGIN      
        1 tcccgatgtg ccgatcggcg aggcgctgct cgaccagcgc aacctcgccg ggatcgggaa
       61 cctgtacaag tccgaggtgc tgttcctgct cggcgtgcat cccgacatgc cggcgggcga
      121 ggcgtccggc gtcgacaacc cgagccaggc ccgagtgtcg tactggtgtc cgtcctgcca
      181 gccggagcct tgagggcgtg gccggcggtt aggcccttcc ggcggtcgag cttgtcggga
      241 ccccgggggc ggcggagtcc gtgatcccga ggctcgttca gcgagctctt cgacgtgctc
      301 gacccgcgat gtcaggcggc gacgttcgtg acgtcggcgc ggacgcggat ggactcggcg
      361 tcaggcgtgc gcacgtggat cgaggtcagc gcggaaacgg cttcctcgcg ggcgatctca
      421 ccgctgacct tggccatgat gaccgacagc ggtacgtcga gggcgcgggt gatggcagcg
      481 aggcattccg acgacgcctc cttctgacca cgctcgatct ccgacagata gccgaggctg
      541 acccgcgcgt cgctggagac ctcccgaagt gtccgaccct gctcccggcg cagctctcgg
      601 agcacgtcac cgaggagacg gcgtaccagg atcatggccg gttcctctcg atcaaggtgg
      661 tcaggcgttg cggtcatgcg gggttgtgtc ggtcaaggct acccggcgca tggatcaccg
      721 ggacccgcca gtgggaacaa cggcaacccc cggcgggatc ttcccgatct catggtccac
      781 gtcggtcggt gtgttccaga gccgtcagca gcagttcgag tacgccgtca acgctggccg
      841 agcggatctg ctgtcgatcg ccagtcagca tgaggcgctg ggacgagacc aggttctctg
      901 gtccggccac ggcgacgtac acctcacccg gcgggtgacc gtcctgcgag tccggtccgg
      961 cgacacccgt ggtcgccacc gcccagtcgg cgcggaacag gtcgcgcgcg gccagcgcca
     1021 tctgctcggc gcactcagca gagatcacgc cgacccgggc gatcacctca ctgtccacgc
     1081 cgaggacatc gaccttgacc tcagtggcgt acgccgcgag gccgccgcgc agcacgtcgg
     1141 acgcgcccgg gatcgtggcg agcgtcgaga ccaccagccc ggcggtcagc gactccgccg
     1201 tggccaaggt cgcggatctg tggtgaaggg cccgcaggca ggcctcggcc acctcgctac
     1261 cggggtcggt catcgacgac tcaggacgcg gcgcgttcgg ctgccttgcc ctcacggcgg
     1321 agccggatcg cgtcccgcac gtagtcggca ccggtcacca gggtcacgac gaccgcggct
     1381 gccatcacga tcactgccag ccaccacagc acgtcgccga ccgacgccca ggcgccgtcg
     1441 aggtcgcgca gcggcaggat gaacgcgctc agcgccacgg cctgcagcgt cgtcttgatc
     1501 ttgccgcccc gactggcggg catgacgccg taccggatca cccagaagcg catcagcgtg
     1561 atgcccacct cgcggccgag gacgaggatc gtgacccacc accacagctc gccgatgatc
     1621 gacagcccga tgaacgccat gcccgtcagc gccttgtcgg cgatcgggtc catcagcttg
     1681 ccgaagtcgg tgatcaggtt gcgacgtcgg gcaagctccc cgtcgatctt gtcggtcaga
     1741 atcgcgacca cgaagaccac gaaggcccag acccgcaagc ccgtgtcttc cccaccatcg
     1801 gaaagcagca gccacccgaa caccggcacg agcaggatgc gcagcgtggt cagcgcgttc
     1861 ggaaggttcc agttcgacac tgggtcgagg tccgccgacg gagtcgcacc ggatccgcct
     1921 gccgcgggat cggagtagcg gtcgtcggcc atgcgctcaa cctattgcgt ccgggcgtcg
     1981 gccacgaggt cgacacccga cgagccggtg acgcgggccg gtacgacgtc gccgactccg
     2041 gcatcggccg agaggttgat gaccgtcgtg gtcccgtcga cctccggccc ttgacacgcc
     2101 gcccggcctt ccccgaccgt gccgtcgatc cgctccagca gcacgtcgac cagttcgccg
     2161 cggcggtctt ccgcccgctg ggcggtgagc tcgtcggcga gacgggtcac gtgctcgacc
     2221 cggtcgcgga cctcgtcctg atcgtggtgc ccgtcgaggc cgaccgcctc ggtcccgtcc
     2281 tcgtcggagt acccgaagac accgacggcg tccaggcgtg cggccgtcag gaagtcactc
     2341 aacacgtcga gatccgcctc ggtctctcca gggaagccga cgatgacgtt gctccggatg
     2401 ccggccgacg gactgtgacg ccggatctcg tcgaccaggc cgaggaaccg ttcggcgtca
     2461 ccgaacctgc gcatgcgccg cagtaccggc gccgaggcgt gctggaacga gaggtcgaag
     2521 tacgacgcga ctcccggtgt cgcggcaatc gtctcgacga gggatggccg catctccgcg
     2581 ggctggaggt aggagacacg cacccggcgt acgccgtcga ccgaggcaag gtcgggtagc
     2641 agcgtctcca ggagccggag gtcaccgagg tccttgccgt acgacgtgga gttctcgctg
     2701 acgaggaaca gctctcgaac cccctcggaa gccagccagc gaccctcggc gacgacgtcc
     2761 tccggcgacc tcgagacgaa cgccccgcga aagctcggga tcgcgcagaa ggagcaccgc
     2821 cgatcgcagc cggacgccag cttcagcggc gccatcggac ccgacccgag ccggcgccgg
     2881 atcgctcgcg gaccactggc cggaccgacg ccggccggga gatcgggcgg cgactgagcg
     2941 tggcccggga ccgagggcgc gacggcggca tgtcgctcga tcggtgtgac cggcaacagc
     3001 ttgcgccgat cgtgcggcgt gtgtgcgccg tgccgatggc cgtcgaggat gtggcggagc
     3061 cgggccgaga tgtccgggta gtcgtcgaat cccagcacgg cgtcggcttc gggtagtgac
     3121 gtggcgagct gttcgccgta ccgctcggcc aggcagccga ccgcgacgac cgccctgacc
     3181 gtcccggcct ccttgaggtc ggcgacggcg agcaacgcgt cgatcgagtc cttcttcgcg
     3241 gcctcgacga agccgcaggt gttcacgacg acggtctcgg catcgctcgc ctcctcgacg
     3301 aggcggaagc caccgagctc gagccgagcc gccagctcct cggagtcgac gtcgttgcgg
     3361 gcgcagccga gcgtcaccag cgccaccgtg gtcgccgagc caggctgcat gctggctgcg
     3421 gggtcggcga tgggcatcgg gtcggtcttc atgagctgcc ctcagtatgc cgccccacga
     3481 ttgcttcgac ccacgcctcg gcggcaactc tcggtgaggt ggcaagtttg gttgcagaca
     3541 cgccggttca ggggacgcgc tcgccacctc ggcggcgacc acggtgaagc ggtcagcggg
     3601 gatgggtggc gcggatgatg cggtgggcgg aactgccggg ttcacccatg aagccgagca
     3661 ggtggccgtt cgaatggaga cggatcacgc caccgttgtc cgcgtgcacg cgcatcgggg
     3721 cgagtccgtg aacccgccgt gcctgcccgt cggtgaggag tccctgccag acgacgtggc
     3781 catggcggtt cttcaccacc acccgcgacg cgccacccac cgccgcgatc cggacggatc
     3841 ccaccggcgg cttggacggc agcagcgacc ggttgcccgg gccgggcgag cccagccctg
     3901 cggagttgtg cgtcggcgcg acacccgagg tcagccgtgg cggatcgtcg gcgaagtact
     3961 tcaccacgcc ccacaccagc acgagcacca gcacggcggc gatgagcccg ccccagttgg
     4021 cgccggacgc gccgccccgg acgagtccgg tcggcgaggt ggcgagctcc gcgccgaaga
     4081 ccgccctcgg gttgatgggc gacgtcgcga aggtttcgtc gtacgtcgcc accaggacgt
     4141 cggcgtccag cccgaggacg cgcgccaaca tccgtaggtg gccccgggcg tagaagtcgc
     4201 cgccgcaggg agtgaagtcg tccacctcga tggactcgat gacgtagggc cggatgcggg
     4261 tccgctcggc gagctcgtcg acgtcgagcc cgacccgttc ccgcgccgac cgtacctgcc
     4321 ccccgatgac caggtcccca gccgcagcag ccggcgcgcc cgagtcggca ccatctcctg
     4381 ttcgcccagc catggcggtg ggctgcccgc ccgctcgcca cctctgcatc gcccgcgcgg
     4441 acaggtcagt cgtcgcgtcg atgatcagcg ccacattgct caccggtgca tcctggtcgc
     4501 gctgtggttg cgtctcgccc aaggtctcgt ccgaggtctc ggccggctgc aactcggcac
     4561 cgtcggcgtc cgcctggcgc ttgagctgtt cgcgttccgg gaggccttcg gtgtcggcgt
     4621 acggctcgga caaggccagc gtgccaagcg tggcgggttc acggccggcc ggcagcgtga
     4681 cctcttcacg ccgtgccgga tgggaccgca gtgcggcgac caccttcgcc agcgggaggc
     4741 tcggcattgc ctcgcgggcg gcggtcagcg ggcgtcgcgg cttgcctggt cgtcctggcc
     4801 cgccatcgtc gtccgcagat gcctgaagtg gttcgtcggc cggctcggca gggccgttcg
     4861 tcaaggccac ggtcggctcg ggctcggact ccgtttcatc gacgtcgacg accggcgctc
     4921 gccccccggc cagcgcaacg agcgtgcccg ggatgtcggc aacgctgacc gtcgtggtga
     4981 gtccgaacgg cacgacgagt ggagcgtcgt agaacaagcg gttcgtcctc gcggccagcc
     5041 gggcgcgtcg gccggcctgt gccacctcct catccgggcc gtggaccagc acggcgacgt
     5101 ggccgtcacg caaccggcct cggcggtcga cctcgacaca ctcgacgtgc gtccagggca
     5161 cgccgatcca tcgtcgcccg agtcgaaccc gcagcccggt cgggtcagcc accaggagcg
     5221 gcgtacgggc gtcgccgaac gccacgccga aacacacggc cagcacaccc aacaccaccc
     5281 cgaccgcgag cggcagaccg tccccggtct ggccgaaccg ccagaggtat cccgccgaga
     5341 gcgcgaacga cgtccccgcc acagccgcca tcagccacgc gcggcggcgc acgaccaggc
     5401 cgtcgtcgga cgaggtgtcc tcacccgacg gaccggttga cacgtcgacg tctggtggct
     5461 cgatcaatgc ctgctgctcc atcagctctc cccccgcagt ccggcgatca cgtcgtcgag
     5521 gtcgtcgggc ttgaccagta cgtcgcgggc cttggacccc tccgacggac cgacgacgcc
     5581 gcggctctcg aggatgtcca tcagccggcc cgccttcgcg aaccccactc ggagcttgcg
     5641 ttggagcatc gaggtcgagc cgaactgcgt cgacacgacc agttcgacgg cctggcacac
     5701 caggtcgagg tcgtctccga tgtcgtcgtc gagctcacgt ctgctctgca ccgacgccgt
     5761 cacgtcgtcg cggtacgtcg gctgcagctg ctgcttgcag tggcccacga cctgcctgat
     5821 ctccgcctcg gtcacccagg cgccctggat gcggccgggc ttgctcgcgc ccatcggcag
     5881 gaacagcgca tcacccttcc cgaccagctt ctcggctccc ggttggtcga ggatgacgcg
     5941 actgtcagcg agcgacgacg tcgcgaacgc caaccggctc ggcacgttcg ccttgatcag
     6001 gccggtgacc acgtcgaccg agggccgctg cgtggccagc acgagatgga tccccgcggc
     6061 acgcgccagc tgggtgatcc gcacgatggc gtcctcgacg tcgcgcggcg acaccatcat
     6121 gaggtccgac agctcgtcga cgacgaccag caggtacggg tacggcgtca gcacccggtc
     6181 gacgccgggc ggcacgcgca cggctccggc ccggacggcc ttgttgaagt cgtcgacgtg
     6241 ccggaaaccg aagttcgcca ggtcgtcgta ccgcaggtcc atctcccgaa cgacccactg
     6301 caacgcctcg gcggccttct tggggctggt gatgatctgg gtgatcaggt gcgggatgcc
     6361 gtcgtacacc gtcagctcca cgcgcttcgg gtcgaccagg atcatccgca cctcgtccgg
     6421 agtggcgcgc atcagcaccg agcagatcat cgagttgatg aagctcgact tgccggaacc
     6481 ggtcgcgccg gcgacgagca ggtgcggcat cttcgtgagg tttgcggcga cgaagccgcc
     6541 ctcgacgtcc ttgccgagac cgatgaccat cgggtggtgg tcgtgacggg ccgacccggc
     6601 gcgcagcacg tccccgaggg agaccagctc ggcgtccggg ttcggaatct cgacgccgat
     6661 agccgacttg ccgggtatcg gcgagaggat ccgcacatcg gcgctcgcga cggcgtacgc
     6721 gatgttcttc gacagtgcgg tgaccttctc gaccttgacg gctgtcccga gctcgacctc
     6781 gtacctcgtc acggtgggcc cgcgggtgta gccggtcacc tgcgcgtcga tgtcgaactg
     6841 ctccagcacc tccgtcagcc ggtccacgat cgcgtccgat gcggccgagc gcgccttgtg
     6901 ggcgctgccg gacctgagta cgtcgttctt cggcagcgtg taggtgatgt cgccggacaa
     6961 gctcagctgc tcgacgcgct gtggcagtgg ggtgtgcggc ggcggctcga gcgggccgtt
     7021 gtccccggtg tcgggcaggt gcggttggct cggccctcct gcctcggtgc gggacgcgac
     7081 caccggctcg gtcggctcgt cgtccgccat cgcgccgacc cggctccggg ggcgcgtacg
     7141 ccgcagcggt tccggccgct ccaccgggac ttcctccacc gcatgggggt cggccggctt
     7201 gcgacccatc accttgtcgc gcagcgccgt cagccgacgc ggtatctggt agaccggtgt
     7261 cgccgtgacc acgagcaggc cgaagaacgt gagcagcacc aacagcggga ccgcgatgac
     7321 cgtgctgcgg aacaggtcgg ccaccatcgc cgaaccgacg tagcccaccg cgccgccggc
     7381 gccgcgcatg gcttcctggc cgccgttggg tcggggcagg ccgtgctgga tgtggatgag
     7441 ccccaagact ccgagcgaga tggccgtcca gccgatgacc ggccgaccgg ctggtccgtt
     7501 caactccggc gctcgcatcg cgcgccacgc ggcaagcagg agcagaaggg ggacggcgta
     7561 cgccgccaag cccaccgcgc cctccatcac ggcgcgaagg acgtcgccga cggagcccgg
     7621 caactgccac cactccgccg cggccacgac gatcgcggct cccaccagtg ccaggccgat
     7681 gccgtcgcgg cggtgttcgg gctcgaggtc gcgtgccgtc gacccgatgc gccgcacgat
     7741 cgcgccgatg acatgggcga tccccaacca gacggctcgc aggccacgtc cgatgccgga
     7801 cacgaacagc gcgatgaggc caggcccacc gcgccgtgcc gaagaccgag agcgcgaacg
     7861 gctgctcgcg ctgcgtttcc tcgacgagga tcgccgggtc tggctaccgg acgtgcccga
     7921 tcgagagcgt gccttgctcc gcgaccgcgg cggggaagac gtacgggtcg ccatggcgtc
     7981 gaggctacgc gatcgtcaca tcagtctcac aggtaacacg ccgctccccc gcggccaccg
     8041 ccttcccgtc cccccggctt gcgtccgtgg aaccggttgt cggggcagcc gcgtcgacgg
     8101 acgtagcccg cggccacgtg cttgcgtccg tggaaccggt tgtcggggca gccgcgtcga
     8161 cggacgtacg cgtcagaagt actcgagcag gtacgccggc cgacccgcga agacgctgtc
     8221 gagctggtcg cgcgactcgg ccgtaccgcc tgccaccaaa ccggcggtga ggagggtcgc
     8281 ggtgggagtt ccggcataca gcgcagccag cccgttcggg ccgagccgca cggccgcgtc
     8341 gtcagaccga acgccctcgt caggcacgaa cgtccccgtc cccccggaga cgaaaagccg
     8401 ccccgtgacg ccgccccccg gcagcagcgg atcgtccacc acgaggccga cctgggtatc
     8461 cgtagcgggg gggtagcctc ggccactcag ggcagcctcg acgtcgagca cacgcagcat
     8521 ccagcgctcc tgtcgaacgt cgcgaagcac agtctcgccg aggatctggt ggatcgggtc
     8581 gtcaggggcg aggtacgcgt gcacggtgtc gacgatggac gagctcgaac cgaccaccga
     8641 ccagagattc ctggccgacg ccggcgaacc ggcaaccagc tggtagacca cgatgtcgcg
     8701 gtcctcccag ccgtagacga cgaagccgtc atgcatcgcg taggcgaaca cggtctccgc
     8761 cgccagctcc tcggcaaact cgtctgcatc gtggtcgcgg acgccgttca cacggccggc
     8821 ggcgtactgc tcccgcatga tcgccagcag gtgctcggcg tcactgggcc cgacctcccg
     8881 cacgtcagga gtctccgcgg ttgtgcgcag gtcgcgcagc aacctcgcct tgatgctcgt
     8941 gcggtgctgg acacccgcga tctcccagcc gttctggcgg tagacgggga ctgtggccgg
     9001 gtacaacgcc gacaccgggt agcccaggtc gcgcccacgt tcgaccatcc cctgcatcaa
     9061 cgcgctgccg actccacggc cgcggtactc cggggccacc accacgcccg cgatgccggc
     9121 catcgcgagg actcgaccgc cccagaactg ccggaacggc cagatcagcg cgcgtgccac
     9181 cagcaggtca ccgtcgtacg ccgccagtgt ccggccttcg tcgatcgcct tctcgacgcc
     9241 cgcgtgccaa cccggcccgg caccctcggg gaggggcccg aacgaccggt tccggacgtc
     9301 gaacgaccgt tcgatgtcgt ccttggcggg gtcgcggatc gtgatcgact gcacggccgt
     9361 gagcctatgg cttcccgcga attgcgggaa agcggttgcc gacacgcggg ccaggaccgc
     9421 acttttcccg caattcgcgg ggaccccggc ggaaggtcag cctgagccga aacagacgaa
     9481 cggggcgggg atcgggccgc cgacgtcgac catcgcggtc gcaagagcgt cggcgggtgg
     9541 gcggccggcg gccaggtagt ggtgcgtggc cgccaacgtg taacaggcga cgtcgtcgtc
     9601 gacgctggcc ggtgacgcga tgacggtcct ggcgccggcg tgcagccagg ccacggtcat
     9661 cccgatggtc tcctcacccc agcggatcga cgaccgaccg agctcgcacg cagacaggat
     9721 caccgtcgac gggatcgcgt cgagctggtc gatgtcgtag ccgaaccacg gtccgtccgc
     9781 gagctccaga ccggagaaca aggggttgtc agcagaatgc cggccgtgcg cggccacatg
     9841 gaacacgtcg accgacgagg ccaggtcgcg caccctggca gcgtcggcgc ggtcctggcg
     9901 cacgatcgtg gcgtgccccc atgccttcgc ggccgcctcg acctcttcgg ccgcgcggtc
     9961 gacccgcgga ccggcgacga gtccggcact ggcggcgcgg tgaccgtcag gtcgcgtcgt
    10021 cagccaggcg gtggccgacc gaggcaccgt gagcggccgg ccgaacagac ctggcagcag
    10081 ggtccacggc actccggcca gcgcaccgga cggtacgacg accagtcgtc ggtcgccgag
    10141 gtgctccagc aacggcgcca ccagacggtc agcaagatct tccagccggg ctcgcaggcc
    10201 ggcttggacc gtcgaccgca gagcctgcgg cagctgggtg gccgacatgt cgaggtcagc
    10261 ctgcatcccg gcgagcaccc ggcgtacctc cgccaggtca ccgagtgact gcagttcggc
    10321 gctgtgcgca tccacgctca gcgcatcgag ccggtcgccg acccggaggt aggcgatgag
    10381 gacggcaccg tcg
//
LOCUS       DATBFZ010000261         7680 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_831946, whole genome shotgun sequence.
ACCESSION   DATBFZ010000261 DATBFZ010000000
VERSION     DATBFZ010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 7680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7680
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_831946"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..368
                     /locus_tag="VH419_08050"
     CDS             <1..368
                     /locus_tag="VH419_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY7043603.1"
                     /translation="GTRAHDMVADHVFRALADATRRDILRRSVRGELSVSRLAELYPM
                     SFAAVQKHVAVLERAGLVTKERRGREQLVCTDPTAVRRARRALDELETAWQGRVDRMT
                     GLLAADGDPQPTFPTRGQD"
     gene            368..862
                     /locus_tag="VH419_08055"
     CDS             368..862
                     /locus_tag="VH419_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003884123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEY7043604.1"
                     /translation="MSVISVDKDFEKLTLTLVADLAAPPERVWELWADPRQLERWWGP
                     PGYPATFEEHDLSPGGSVTYFMTGPDGEQYRGWWRVASINAPKSLEFSDGFADKDGIP
                     TEDMPVTTIQMELMEHEGGTRMELRSVFDSKEQMEQLDGMGMTEGLSQAVGQMDALLT
                     EDGR"
     gene            859..1470
                     /locus_tag="VH419_08060"
     CDS             859..1470
                     /locus_tag="VH419_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020576656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEY7043605.1"
                     /translation="MRKLTVNTFLTLDGVMQAPGGPEEDDSSGFNHGGWSVNYWDDTM
                     NQAMADATSTPFAMVLGRKTYDIMAAYWPTAPEEAGGTVFNEATKYVASRSRRTVDEW
                     ANSMLIEGDAAEGLAALKQQDGPELRVSGSADLIQTLLRHKLVDEYWLWVFPVVIGSG
                     KRLFADGTIPSALKLVDHKVSTTGVVMGVYQPAGEIETGSFAE"
     gene            complement(1551..3785)
                     /locus_tag="VH419_08065"
     CDS             complement(1551..3785)
                     /locus_tag="VH419_08065"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEY7043606.1"
                     /translation="MSRHQKANLAARMGRWSAGHWKTATLGWLAFVLVAFALGGMIGT
                     KFIDPNTSGPGESGRMDRILDAGFRQPAGESVLVQSESLQTSDPAFRAAIKDVVTGIS
                     GLENVQHVRSPLAPANAGQIAPSGTAALVEFEIRGDADTAVDKIDPILTKVAEMQRAH
                     PQLYIGEFGDASSMHAIDTAFADDLASAGLFSLPITLIILVVAFGALVAAGIPLLLAL
                     TAVFATFGLMAVPSQVLPVAPEASALVLLVGLAVGVDYSMFYLKREREERAAGRSERA
                     ALEAAAATSGRSVLISGLTVMVAMAGLFLIGDGTFASFGLATMMVVAIAMLGSLTVLP
                     ALLSRLGDKVDRLRVPFVGRLRRADGEGRIWGAIIDRVLRRPLLSAVLAGGLLLALAA
                     PAIQLHISTPGPDTFPQSLDVVKTYNRMQEAFPGTALPANVVVKAPNVTAPAVQDAID
                     QLEQRAVESGLMYEPVTVEVNQDSTVANITVPIKGTGTDDGSLAALATLRERIVPETT
                     GVLPDTESGVTGLSAQWKDSADQMKSTLPLVFGFVLLFAFVLMLVAFRSLVVAAKAIV
                     LNLLSVAAAYGVLVLVFQHGFAKGLLGFSSTSGIDPVVPLLLFVILFGLSMDYHVLVI
                     GRIRETYDRGADMDDAIAHGIKSTAGVVTSAAVVMVAVFSIFGMLSMLFFKQFGVGLA
                     TAILLDATIVRAVLLPATMKLLGERNWCLPGWLEWIPRLEHSEPDSDEVEPVDDRVPQ
                     PTRA"
     gene            3969..5135
                     /locus_tag="VH419_08070"
     CDS             3969..5135
                     /locus_tag="VH419_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEY7043607.1"
                     /translation="MGGLRGVARRRGFDALIALMTVAAALEVAVRRDSAEAPRTTLWF
                     SVPAAALMVAPLLWRRRFPFLAPVAVWLVGAAVSFFDGRLVVFAATASVAGMVASFLI
                     GNLVDTVQSRIGLVIMIGSAAIVVYNDPAHDLTEFIFIPLFFVLSWLAGAALRKRAEQ
                     AESAELRAVQAERDRDAAMRVAVAEERARIARELHDIVAHAMSVMVLQVGAVRHKLPD
                     ELAEHRDILRGVEQAGRTALAEMRRLLAAMRLAGEEAELSPQPGLGSIDSLLKEVGAA
                     GLPVAMHVEGEPFPLPRAIDLSAYRIVQEGLTNALKHARASNADVTVRYGADSLDIEV
                     RDNGYGASTSDSLGHGLVGVRERVKIYGGEMTAGAAADGGFVLSTRLPVSGDGR"
     gene            5132..5830
                     /locus_tag="VH419_08075"
     CDS             5132..5830
                     /locus_tag="VH419_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020576049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY7043608.1"
                     /translation="MTIRVLVADDQSMVRAGFCMLLSGERDIEVVAEATNGIEAVEKA
                     ARFDPGVVLMDIRMPELDGLEAARRILAADRSARILILTTFDLDDYVYEALKAGASGF
                     VLKDDPPEQLIAAIRTVAAGDALLSPTITRRVIQQFTRIPRADRPKQLDELTEREQEI
                     LRLIARGLSNAEIAQTLFIGDTTVKTHVTHILQKLNLRDRVQAVVLAYQTGLVETDVT
                     RPTDATAPRGGAAG"
     gene            complement(5854..6687)
                     /locus_tag="VH419_08080"
     CDS             complement(5854..6687)
                     /locus_tag="VH419_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020575987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY7043609.1"
                     /translation="MTASPATSSGTGNGQYHEVNGLDMYVETHGSGRPLVLLHGGLFS
                     GEMFGPVLPALADHHQVIVPDLQGHGRTADIDRPLDVTLMADDIAALIDHLGLDQPDI
                     VGYSLGGGVALHTAAKYPDKVRRAVVASAHTRRDAVYPEMLAQQEQVNAATAEFIKDT
                     PMYELYQRVAPRPEDFPRLLDKIGEAMARDFDLAETVRGLRVPTLIVAADADMAPPSH
                     YVEAFKLLDGGVRDGGWAGEGRPAGGHALAILPGLTHYNAATSPLFAAVTLAFLDEQH
                     P"
     gene            6804..7583
                     /locus_tag="VH419_08085"
     CDS             6804..7583
                     /locus_tag="VH419_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020580086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEY7043610.1"
                     /translation="MTEFFGADLRHSRFERVDLTGSQFWASDLSDSRLRGTDLDRVVM
                     RGVELVDVDISGEIVNVTVNGVDIVPLVEAELNRRYPERVKMRPSDAAGFREAWDILE
                     RLWDETVDRARRFDPELLHQSVDGEWSFIETLRHLVFATDSWIRRGIHGDPAPWHPLA
                     LPWDEMADTPGVPRDRDARPSLDEVLELRRDRMAGVREVIDGLTDDTLDSETEPVEGV
                     GWPPAGRTFPVRKVLSIILNEEWEHRLYAERDLGILQVRAQ"
BASE COUNT         1281 a         2502 c         2650 g         1247 t
ORIGIN      
        1 acggcacaag ggcgcacgac atggtggcgg accatgtctt ccgcgccctg gccgacgcca
       61 cccgacgcga catcctgcgc cgttccgtcc gcggtgagct gtcggtgtcg cggctggccg
      121 agctgtaccc catgagcttc gcggcggtac agaaacacgt cgcggtgctg gagcgggctg
      181 ggctcgtcac caaggagcgt cggggtcgcg agcagctcgt ctgcaccgac ccgaccgcgg
      241 tacgtcgcgc acggcgggcc ttggacgagc tcgagaccgc ctggcaaggt cgagtagacc
      301 gcatgaccgg gctgctcgcc gccgacggcg atccacaacc cacgttcccg acgagaggac
      361 aggactgatg agcgtcatca gcgtcgacaa ggacttcgag aagctgaccc tcacactggt
      421 cgccgatctc gccgcgccgc ccgagcgagt ctgggagctc tgggctgacc cgcggcagct
      481 cgagcgctgg tgggggccgc cgggctaccc ggcgacgttc gaggagcacg acctgagtcc
      541 gggcggaagt gtcacgtact tcatgaccgg tcccgacggc gagcagtacc gcggttggtg
      601 gcgggtggcg tccatcaatg cgccgaagtc cctcgagttc agtgacgggt tcgccgacaa
      661 ggacgggata ccgaccgagg acatgccggt cacgacaatc cagatggagc tcatggagca
      721 cgagggcggc actcggatgg agctgcgttc ggtcttcgac tcgaaggaac agatggagca
      781 gctcgacggc atgggtatga cggagggcct ctcgcaagcc gtcggccaga tggacgcact
      841 cctcacggag gacgggcgat gaggaagctg accgtcaaca cgttcttgac gctcgacggg
      901 gtcatgcagg cgcccggcgg tccggaggag gacgacagca gcggcttcaa ccacggcggc
      961 tggtcggtga actactggga cgacacgatg aaccaggcca tggcggacgc gacgagcacc
     1021 ccgtttgcga tggtcctcgg ccgcaagacc tacgacatca tggcggcgta ctggccgacc
     1081 gcgccggagg aggccggcgg cacggtgttc aacgaggcca cgaagtacgt cgcctcgcgc
     1141 agtcgccgaa cggtggacga gtgggccaac tcgatgctca tcgagggaga cgccgccgag
     1201 gggctggccg cgctgaagca gcaggacggg ccggagctgc gagtgagcgg aagcgccgac
     1261 ctgatccaga cgctgctgcg gcacaaactc gtcgacgagt actggctgtg ggtcttcccg
     1321 gtcgtcatcg ggtcgggcaa gcggttgttc gccgacggca cgatcccgtc ggcgctgaag
     1381 ctcgtcgacc acaaggtctc caccaccggc gtggtgatgg gtgtgtacca accggcgggg
     1441 gagatcgaga cgggttcgtt cgccgagtga ggatcgggca tcgtctccac tgcggtcccg
     1501 tgtccggccg cggatggagg cgaccggaca cggaacggct gcggtggtgc tcaggccctc
     1561 gttggctgcg ggacgcggtc gtccactggt tcgacctcat cgctgtcggg ctcgctgtgc
     1621 tcgagccgag gaatccactc cagccagccg ggcaggcacc agttccgttc gccgagtagc
     1681 ttcatcgtgg ccgggaggag cacagcgcgc acgatggtgg cgtccagcag gatcgcggtc
     1741 gccagtccga cgccgaactg cttgaagaac aacatcgaga gcatcccgaa gatcgagaag
     1801 accgccacca tgacgacggc tgcgctggtg acgactccag cggtgctctt gatgccgtgc
     1861 gcaatcgcgt cgtccatgtc ggcgcctcgg tcgtacgtct cacggatgcg gccgatgacg
     1921 agcacgtggt agtccatcga gaggccgaac aggatcacga acagcagcag cggaaccact
     1981 gggtcgatcc cggatgtcga gctgaagccg agcagacctt tggcgaatcc gtgttggaag
     2041 acgagcacga gtacgccgta ggcggcggcc acggacagca ggttgagcac gatggccttg
     2101 gctgcaacaa ccagggagcg gaacgcgacc agcatgagca cgaacgcgaa gaggaggacg
     2161 aacccgaaca cgagcggcag cgtcgacttc atctggtccg cggagtcctt ccactgtgcg
     2221 ctcaaccccg tgacaccgga ctcggtgtct ggaagcacac ctgtggtctc gggcacgatg
     2281 cgttcacgca aggtagccag agcggcgagt gatccgtcat cggttccggt ccccttgatc
     2341 ggaacggtga tgttggcgac ggtgctgtcc tggttgacct cgactgtgac cggctcgtac
     2401 atcaagccac tttcgaccgc acgctgttcg agctgatcga tcgcgtcctg cacggccggt
     2461 gccgtcacgt tcggcgcctt gacgacgacg ttcgccggca acgccgtacc tgggaacgcc
     2521 tcctgcatcc ggttgtacgt cttcacgacg tcaagcgact gcgggaacgt gtccgggcct
     2581 ggggtggaga tgtgcagctg gatggccggt gcggccagcg ccagcagcag gccgccggcg
     2641 aggaccgccg agagcaacgg ccgacgcagg acccggtcga tgatcgcgcc ccagatccgt
     2701 ccttcgccgt cggcgcgccg cagtcggccg acgaacggaa cgcggagtcg gtcgaccttg
     2761 tcgccgagcc tggagagcag ggccggcagc actgtcagcg aaccgagcat cgcgatggcg
     2821 acgaccatca tcgtggccaa accgaacgag gcgaacgtgc cgtcgccgat gaggaagaga
     2881 ccggccatcg ccaccatcac cgtgagaccg gagatcagca cggagcggcc ggaagtggct
     2941 gcggcggctt ccagagccgc ccgctcactg cgcccggcgg cacgttcctc gcgctcccgc
     3001 ttcaggtaga acatcgagta gtcgaccccc accgcaagcc cgaccaggag cacgagggcc
     3061 gaggcttcgg gggccaccgg cagcacctgg ctcgggacgg ccatcagtcc gaacgtcgcg
     3121 aagacggccg tcaacgcgag aaggagcggt atgccggctg caaccagggc cccgaaggca
     3181 accacgagga tgatcagtgt gatcgggagc gaaaagagtc cggcgctggc caggtcgtcg
     3241 gcgaaggcgg tgtcgatggc gtgcatcgag cttgcgtccc cgaactcccc gatgtagagc
     3301 tgcgggtgtg ctcgctgcat ctcggcgacc ttggtcagga tcggatcgat cttgtcgacg
     3361 gccgtgtcgg catcgccccg gatctcgaac tcgaccaggg ccgcggtccc gctcggggcg
     3421 atctggccgg cgttggccgg tgcgagcggc gaccgaacat gttgcacgtt ttcaaggccg
     3481 gagatacccg tcacgacgtc cttgatggca gctcggaaag cgggatcact ggtctgaagc
     3541 gactcgctct gcacgagcac gctctcaccg gcgggttgcc tgaagcccgc atccaggatc
     3601 ctgtccatgc ggcccgactc gcccggcccg gatgtgttcg ggtcgatgaa cttcgttccg
     3661 atcatgccgc cgagtgcgaa ggcgacgaga acgaacgcga gccagccgag ggttgccgtc
     3721 ttccagtgcc cggcactcca gcgacccatc cgggcggcaa ggttggcctt ctggtgacga
     3781 gacatctcca tcctccttcg aaaccagtcg gtctccgcgg gcggatccga tctgtacgtc
     3841 gcgacgctag gcgcgccgag ctccgaaagc gtccgcgtca ggtccgatcc gactcgtgcc
     3901 ggatgacgat tccgttgtcc tactcgagga ggaggcgcca ccgagtcggc gtgcagtacg
     3961 ttcacgacgt gggagggctc cgaggcgtcg ctcgtcggcg cgggttcgat gcgctcatcg
     4021 cgctcatgac agtcgccgcc gcactcgagg tcgccgtgag gcgggactcg gcagaggcgc
     4081 cgcgtacgac gttgtggttc agtgttccgg cagctgcgct catggtggcg ccgttgctct
     4141 ggcgccgccg attccctttc ctcgccccgg tggccgtctg gctggtcggc gcagccgtgt
     4201 cgttcttcga cggccggctc gtcgttttcg ccgccacggc atcagtggca ggaatggtgg
     4261 ccagcttcct catcggaaac ctcgtcgata cggtgcagtc gcgtatcggg ctcgtcatca
     4321 tgatcggcag cgctgcgatc gtggtctaca acgaccccgc ccacgacctg accgagttca
     4381 tcttcatccc gctgttcttc gtgctcagct ggcttgccgg cgctgccctt cgcaagcgcg
     4441 ccgagcaggc cgagtcggcg gagttgcgcg ccgtccaggc cgaacgcgac cgggacgcgg
     4501 cgatgcgtgt cgcggtggca gaggagcggg cgcggatcgc ccgcgagttg cacgacatcg
     4561 tcgcgcacgc catgagcgtg atggtcctcc aggtcggggc cgttcggcac aaacttccgg
     4621 acgagctcgc cgagcaccgc gacatactcc ggggtgtcga gcaggcgggc cgcaccgcgc
     4681 ttgccgagat gcgtcgtctg ctggctgcca tgcggctcgc gggtgaggag gccgagctga
     4741 gtccccaacc tggtctcggc agtatcgact cgctgctgaa ggaggtcggg gcagccggtc
     4801 tgcctgtcgc catgcacgtc gagggcgagc cgttcccgct gccgcgagcg atcgacctgt
     4861 cggcgtaccg catcgtccag gagggcctca ccaacgcgct caagcacgca cgagccagca
     4921 acgcagatgt caccgttcgc tatggcgctg actcgctgga catcgaggtc cgcgacaacg
     4981 gctacggcgc gtccacgagt gacagcctcg gtcacgggct cgtcggcgtc cgcgagcgcg
     5041 tgaagatcta cggcggtgag atgaccgcag gggccgctgc cgatggtggc ttcgtcctca
     5101 gcacccgcct cccggtgagc ggcgacgggc gatgaccatc cgggtactcg tcgccgatga
     5161 ccagtcgatg gtccgtgccg gtttctgcat gttgctgtct ggtgagcgtg acatcgaggt
     5221 cgtggccgaa gcgaccaacg ggatcgaggc cgtcgagaag gcggcgcggt tcgatcccgg
     5281 cgtggtgttg atggacatcc ggatgccgga gctcgacgga ctcgaggcgg ctcggcgtat
     5341 cctcgccgct gaccgctccg cgcgaattct gatcctcacg acgttcgacc tcgacgacta
     5401 cgtctacgag gcgctcaaag ccggcgccag cgggttcgtc ctcaaggacg acccgcccga
     5461 acagctgatc gcggcaatcc gcaccgtcgc cgccggcgac gctctgctgt cgccgacgat
     5521 cacgagacgg gtgatccaac agttcacccg cattcctcgc gctgaccgcc ccaagcagct
     5581 cgacgagctc accgagcgtg agcaggagat ccttcgcctg attgcccgcg gtctttccaa
     5641 cgccgagatt gcgcaaaccc tgttcatcgg cgacacgacg gtcaagacgc acgtcacgca
     5701 catcctgcag aagctcaacc tgcgcgatcg cgtccaggcc gtcgtcctcg cgtaccagac
     5761 agggctcgtc gagaccgacg taacacgacc cacggacgca acggcaccac ggggtggggc
     5821 cgccggttga acgacctcga cgagatcgga acgtcagggg tgctgctcgt cgaggaacgc
     5881 cagggtgacg gcggcgaaca gcggtgaggt ggccgcgttg tagtgcgtca ggccgggcag
     5941 gatcgccagc gcgtgcccgc cggcggggcg gccttcgccg gcccagccgc cgtcacgcac
     6001 gccgccgtcg agcagcttga acgcctcgac gtagtggctt ggcggagcca tgtcggcgtc
     6061 cgcggcgacg atcaacgtcg gaacccgcag gccgcgcacc gtttcggcga ggtcgaagtc
     6121 cctcgccatg gcttcgccga tcttgtcgag cagccgcgga aagtcctcgg gacgcggtgc
     6181 cacgcgttgg tacagctcgt acatcggtgt gtccttgatg aactccgccg tggccgcatt
     6241 cacctgctcc tgttgagcca gcatctcggg atagacggcg tcacgacggg tgtgggctga
     6301 ggcgacgacg gcccggcgta ccttgtccgg gtacttcgct gcggtgtgca gcgcgacgcc
     6361 gccaccgagc gagtagccga cgatgtccgg ctggtcgaga ccgagatggt cgatgagcgc
     6421 cgcgatgtcg tcggccatca gcgtcacgtc gagtggcctg tcgatgtcag cagtacgccc
     6481 gtgtccctgg aggtccggca cgatcacctg gtggtggtca gccaaggccg gaaggaccgg
     6541 gccgaacatc tcgcccgaga acagtccgcc gtggagcagg accagaggtc gcccgctgcc
     6601 gtgcgtctcg acgtacatgt cgaggccgtt gacctcgtgg tactggccgt tgcctgttcc
     6661 gctgctcgtc gctggggatg cggtcacggt tcgtctcctt gcgcctcgtg cgtcgccgac
     6721 atggatcaga ccactgagcc actcaggact catcgccagt ctgctttcct gcggtcgtcg
     6781 gcggcggccg ttacggtcac gacatgactg agttcttcgg tgcggacctg cggcattcgc
     6841 ggttcgagcg cgtcgacctc actggctcgc agttctgggc cagcgacctg agtgacagcc
     6901 gcttgcgcgg caccgatctg gaccgtgtcg tgatgcgcgg cgtcgagctg gtcgacgtcg
     6961 acatctctgg cgagatcgtg aacgtgacgg tgaacggtgt cgacatcgtc ccgctcgtcg
     7021 aggccgagct gaaccggcgt tatcccgaac gagtgaagat gcgtccgagc gatgccgcgg
     7081 gcttccggga ggcctgggac atcctggagc ggctctggga cgagacggtc gatcgtgccc
     7141 gccggttcga ccccgagctc ctgcaccagt cggtcgacgg tgagtggtcc ttcatcgaga
     7201 cgctccgcca cctggtcttc gccacggact cgtggatccg gcgcggcatc cacggcgacc
     7261 cggccccctg gcaccccttg gccctgccgt gggacgagat ggccgacacg cctggtgtcc
     7321 cgcgcgaccg tgacgcgaga ccgtcgctcg acgaggtgct cgagctccgc cgtgaccgca
     7381 tggccggcgt acgcgaggtg atcgacggtc tgactgacga cacgctcgac agcgagacgg
     7441 agccggtcga gggcgtcggg tggccaccgg caggccgcac cttcccggtg cgcaaggtct
     7501 tgtccatcat cctcaacgag gagtgggagc accggttgta cgccgagcgg gacctcggca
     7561 tcttgcaagt gcgtgcgcag taagtggatc ggtcacccca ctgggtgaga ccgtccggtt
     7621 ggctggtgtc cgctgcggac tgtccggttc cggcggtcga gcttgtcgag accccggacc
//
LOCUS       DATBFZ010000262         6621 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_3697795, whole genome shotgun sequence.
ACCESSION   DATBFZ010000262 DATBFZ010000000
VERSION     DATBFZ010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 6621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6621
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3697795"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(150..968)
                     /locus_tag="VH419_08090"
     CDS             complement(150..968)
                     /locus_tag="VH419_08090"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEY7043611.1"
                     /translation="MKKLLGVLGVTALCAGLGSAVPAPAEATPANATASVLTGSSASP
                     SSAVAAQAAPRHRFTMLDPFARETVHRGMSDQGVHKIAHVRELQYRLRWVGVFHVDVT
                     GTFGDITKAAVKRYQKREGLQVSGVATHATWAHLLRDTIRHASRIPKVCRHKGWHICY
                     DRSMHQVVLLHNGEFRNTWLVRGGASDTPTRLGTHRVYYRDIDHKSQTFNGSPMPYSQ
                     FFDGGEALHGSGFMTDPFVDHSHGCINMYIEDARLLWRMTHDKRLIVTVYGAWD"
     gene            1335..1730
                     /locus_tag="VH419_08095"
     CDS             1335..1730
                     /locus_tag="VH419_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3040 domain-containing protein"
                     /protein_id="HEY7043612.1"
                     /translation="MPLSEEEQRLLEQMEEALAAEDPKFASALRGAASRQRHKRITIA
                     GIAGFVVGIAILMTGAVQRLPWLGVVGFLVMLGCAYLALTHWRKMSEPDLPDNVHPIG
                     KPKGRTAASKPTGFMNRMEDRWRRRRDEY"
     gene            complement(1847..4162)
                     /locus_tag="VH419_08100"
     CDS             complement(1847..4162)
                     /locus_tag="VH419_08100"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HEY7043613.1"
                     /translation="MRASFGERYRLVFLSWLTTVMTSFAFLPTLTQKSFVITAAFVAA
                     LLVLVGVGLRALRAPALVVLLVQLVALVEIMLVGFAEQLKFGLFPTAATFDYLNDKIT
                     RGIDVANHYQAPAPPSAGLTMMLVFYIGLIAVLVDFLAVTVNRVPLAGLPLLALYSVP
                     VASLPNGVSFVLFLPGAIGYVAMIMVDERDRLAHWGRLVARDLTPDPEGRIDTAGLTA
                     TGRRISSVALATAVVVPVFIPVLSTTLFEGSGSGIGNGTGDTLSFSDPMVSLAASLKR
                     KEAVDVLRVDGDVRPQYLRLATLDRPGPNAWSVTPINLSDTLPANSVLPGVPGLDGDV
                     ATTSHQMRIETTDAFPDDSAWLPIPYNVRSVGVGDDWAYVPSDQTITANARLAGSLVN
                     SYEILYATVEPTQEELESAGPPPPDIVKRYTTVPPGVPEIVGDLARTITSGVNNHYLQ
                     AQMLQSWFRDSGNFTYTLNAGYGYGYQAMAKFLTERRGFCQHFAATMAMMARELGIPS
                     RVAVGFLEPDRVDGDSWVLTSHNVHAWPELYFEGVGWVRFEPTPSIGAPFPAYAPRSD
                     LTPETNTPSVNPSTEITDTTKGQLQTAGATTDGGASGGGGTGGALPSRWWLVGGLVVV
                     LALLPAVSRLAIRRRRLTRPVEEGEAAESAWTELRDHIRDLRLPWTGSMTPRARQRAV
                     EPMLEGDAGGVAALQRLSLTVERARYATSVPPGSTPAADAREVMAVISRAAEPGQRVK
                     ALLWPSSLLPDLRAGWASLRDRMRRHSPIDE"
     gene            complement(4159..5469)
                     /locus_tag="VH419_08105"
     CDS             complement(4159..5469)
                     /locus_tag="VH419_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEY7043614.1"
                     /translation="MRSALSTLTTRGRAFFSAGVAASVCAIILGQRDLLRVGILLVML
                     PLVTSLLANRARYLLSCAREITPTRVQAGQTATVALHLQNPGRVPTGLMLLEDQVPYV
                     LGSRPRFVLDQLRPRWQRSMVYTVRSEVRGRYTIGPLTVRLSDPFGFVELTRAFTARA
                     ALVVTPVVVELPPVQLSGDWSGTGDNRPRAFAAAGTEDVTVREYRMGDDLRRIHWRST
                     ARADELMVRREEQPYQSRATILLDTRDIAHRGSGPASSFEYAVSACASIGAHLSGQGF
                     TIRLFTDEARGGDVTWHDRGLSGPVELQLLLDSLAVIKTSARAHVADASAERNASGLV
                     IAVLGAVTPTDVAALATLRAGSTRALAVLVDVGAWARPGRVGEPDIVPVEDQALLLRR
                     NGWAVVVAGPGDRLPTVWKDLANTTELRRDFTAETRASEAGPAA"
     gene            complement(5492..6460)
                     /locus_tag="VH419_08110"
     CDS             complement(5492..6460)
                     /locus_tag="VH419_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017933874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEY7043615.1"
                     /translation="MSSFDTLASVTSRVGDNIEKVIEGKRDVIRMSITVLLAEGHLLI
                     EDVPGVGKTMLAKALARSIDCTVKRIQFTPDLLPSDVTGVSVYNRQTLEFEFRPGGVF
                     ANIVVGDEINRASPKTQSALLECMEERQVTVDSKTYELTAPFMVIATQNPIEMEGTYP
                     LPEAQRDRFMARVSMGYPVERSELDMLDAHSAANPLDDLEPVTDGGEIAKLIDIVRTV
                     HVSEPVKQYAIDITSATRRSPDLRLGASPRASLHLVRAARAHAALEARDYVIPDDIQL
                     LAVPVLAHRLITGLEAQMGGRGADQVLTALLRQIAVPESTSANQSY"
BASE COUNT         1107 a         2210 c         2277 g         1027 t
ORIGIN      
        1 gtcgagcttg tcgagaccct cgatctcgac aagctcgatc agcggaggca ggtggcttga
       61 ccgcgtggat agctcacagc cgccagcgcc gggaatccca ggttgcttct tgagccggac
      121 ccagcgactc cgtcgcccgg gtccgtccat caatcccagg cgccgtagac ggtgacgatg
      181 aggcgcttgt cgtgggtcat cctccacagc agccgggcat cctcgatgta catgttgatg
      241 cacccgtggc tgtggtcgac gaacggatcg gtcatgaacc ccgacccgtg cagcgcctca
      301 ccaccgtcga agaactggga gtagggcatc gggctgccgt tgaacgtctg cgacttgtgg
      361 tcgatgtcgc ggtagtacac ccggtgggtg ccgagccggg tcggtgtgtc ggaggcaccg
      421 ccacgcacca gccaggtgtt gcggaactcg ccgttgtgga ggagcaccac ctggtgcatg
      481 gaccggtcgt agcagatgtg ccagcccttg tgccggcaca ccttcgggat ccggctggca
      541 tggcggatcg tgtcccggag cagatgcgcc cacgtcgcat gggtcgccac ccctgacacc
      601 tggaggcctt cgcgcttctg gtagcgcttg accgccgcct tcgtgatgtc accgaacgtg
      661 ccagtgacgt cgacatggaa gacgccgacc cagcgcagcc ggtactgcag ctcgcgcacg
      721 tgggcgatct tgtggacgcc ctggtcggac atgccacggt ggacggtctc gcgcgcgaac
      781 gggtcgagca tcgtgaacct gtgccgcggc gccgcttgtg cagccaccgc cgacgaggga
      841 gatgcggacg agccggtgag aacagacgcc gttgcgttgg ctggtgtcgc ctcggccggc
      901 gccggtacgg cggagccgag accggcgcac agggcagtga cgccaaggac ccccaagagc
      961 ttcttcacgg tgcttccttc gtgcgccggg gcgcggtgag tacacgacag acgggactgc
     1021 gtggagcggc aaaccggccg gtgtgggccg tcacggctca ggaaccaaac gctcgccggc
     1081 agataccggg ttcctcgagc cgcgccgaaa gggccggcca agcctaccgt cctgctcgaa
     1141 gaattgcccg cttcgatcgg gttcggtgga cggccggacg gcatcccccg gaagcgccga
     1201 cccatccccc tcacctatga gacgttcgaa tgaccaattt ggttggctgg gtttcgcagg
     1261 ccggttgccg tgcttagact gaaagagaac gacccgacgc ccctcgtcgg ctgaatcgtc
     1321 ggttggagga catggtgccg ctctctgaag aagagcagcg tctgctcgag cagatggaag
     1381 aggcgctggc cgctgaggat ccaaagttcg cttctgcgct gcgaggcgcc gcctcacgtc
     1441 agcgtcacaa gcggatcacc atcgccggga tcgccgggtt cgtcgtcggc atcgccatcc
     1501 tgatgaccgg tgcggtccag cggctgccct ggctcggggt cgtcggcttc ctggtcatgc
     1561 tgggttgcgc ctacctcgcg ctgacgcact ggcggaagat gtccgaacca gacctgcctg
     1621 acaacgtgca tccgatcggc aaacccaaag gccggaccgc ggcgagcaag ccgaccggct
     1681 tcatgaaccg gatggaagac cgctggcgcc gccgccgcga cgagtactga cctccaccac
     1741 accacccgct cctgggtgca cgcccggctg gaccggttgg tccggccggt ttgctatgtg
     1801 tcggcccggc gtccacaacg cgtgtcgcgg cgtacgccgt tcggcatcac tcgtcgatcg
     1861 gtgagtgtcg ccgcatccga tccctgagcg acgcccagcc cgcccgcagg tccgggagca
     1921 acgatgacgg ccacagcagc gccttgacgc gctgaccagg ctcggcggcc cgcgagatca
     1981 ccgccatcac ctcccgggca tcggccgccg gcgtcgagcc gggaggcacc gaggtggcgt
     2041 agcgggcccg ctcgaccgtc agcgacagtc gctgcagggc agcgaccccg cccgcgtctc
     2101 cctccagcat cggctccacc gcacgttgtc gcgcccgcgg cgtcatcgag ccggtccacg
     2161 gcagccgaag gtcgcggatg tggtcgcgga gctctgtcca cgcggactcg gcagcctcgc
     2221 cctcttcgac cggacgcgtc agtcgccggc gccgaatcgc cagccgggag acagccggaa
     2281 gcagcgccag caccaccacg agcccgccaa ccagccacca ccgcgacggc agcgcaccgc
     2341 ccgtgccccc gccaccggat gcccccccat ccgtggtcgc accggcggtc tggagctggc
     2401 ccttcgtggt gtcggtgatc tctgtcgagg ggttcaccga cggcgtgttc gtctcgggcg
     2461 tcaggtccga cctcggtgcg tacgccggga acggcgcccc gatcgacggg gtcggctcga
     2521 accgaaccca gccgacgccc tcgaagtaca gctccggcca cgcgtgcacg ttgtgcgacg
     2581 tgagcaccca gctgtcaccg tcgacgcgat ccggctccag gaatccgacg gccacccggg
     2641 aagggatccc gagctcgcgc gccatcatgg ccatcgtcgc cgcgaagtgc tggcagaatc
     2701 cgcgccgctc ggtgaggaac ttcgccatcg cctgatagcc gtacccgtag ccggcgttca
     2761 gcgtgtacgt gaagttgccg ctgtcccgga accagctctg cagcatctgc gcctgcaggt
     2821 agtggttgtt gacaccagac gtgatcgtcc gggccaggtc accgacgatc tccggtacgc
     2881 cgggcgggac cgtcgtgtag cgcttcacga tgtcgggcgg cggcgggccg gcgctctcga
     2941 gctcctcctg cgtgggctcg accgtggcgt acaggatctc gtagctgttg accagcgaac
     3001 cagccagccg agcgttggcg gtgatcgtct ggtcactcgg gacgtatgcc cagtcgtcgc
     3061 cgacccctac agatcgcacg ttgtacggga tcggcagcca ggcgctgtcg tccgggaagg
     3121 cgtccgtggt ctcgatccgc atctgatggc tggtggtggc gacgtcgccg tcgagcccgg
     3181 ggacgcccgg cagcacggag ttggccggca gcgtgtcaga caggttgatg ggtgtgacgc
     3241 tccaggcgtt cggtccgggg cggtcgagcg tcgccagtcg gaggtactga ggtcggacgt
     3301 cgccgtccac ccgcaacacg tcgaccgcct ctttgcgttt caggcttgcg gcaaggctca
     3361 ccatcgggtc ggagaagctc agcgtgtcac cggtcccgtt gccgatgcca ctccccgagc
     3421 cctcgaagag cgtggtggac aggaccggga tgaaaaccgg cacgaccaca gctgtcgcca
     3481 gggcgaccga cgagatccgg cgtcccgtcg cggtcaagcc tgcggtgtcg atcctgccct
     3541 cgggatcggg tgtgaggtcg cgggcaacga gccgtcccca gtgcgccaac cggtcgcgtt
     3601 cgtcgaccat gatcatcgcc acgtagccga tggcgccggg gaggaagagc acgaacgaga
     3661 caccgttcgg aagcgacgcg accggaacgg agtacagcgc cagcagcggg agacccgcca
     3721 gcgggacgcg gttcacggtc acggcgagga agtccacgag gacggcgatc agcccgatgt
     3781 agaagaccag catcatcgtc agcccggcgc ttggcggggc aggcgcctga tagtggttgg
     3841 cgacgtcgat gccgcgggtg atcttgtcgt tcaggtagtc gaacgtcgcg gcggtcggga
     3901 acaacccgaa cttcaattgc tcggcgaagc cgaccaacat gatctcgacc aacgccacca
     3961 gctggacgag caggacgacg agtgcgggtg ctcgcagtgc gcgcagcccg acaccgacga
     4021 gcacgagcag agcagcgacg aacgcggcgg tgatgacgaa gctcttctgg gtgagcgtcg
     4081 gcaggaacgc gaacgacgtc atcaccgtgg tgagccacga caggaacacc agtcggtacc
     4141 gctcgccgaa ggatgccctc atgcggccgg ccctgcttcg gaggcacgag tctcggccgt
     4201 gaagtcacgg cgcagctcgg tggtgttcgc caggtccttc cagacggtcg gcagccggtc
     4261 ccccggaccc gcgacgacca cggcccaccc gttccgccgc agcagcagtg cctggtcctc
     4321 gaccggcacg atgtccggct cgcccacccg gccgggtcgc gcccaggcgc cgacgtccac
     4381 gagcacggcg agggccctgg tggatccggc gcggagggtt gccagcgcgg cgacgtcggt
     4441 cggtgtgaca gcacccagca cggcgatcac gagtccggag gcgttgcgtt cggccgaggc
     4501 gtcggcgacg tgcgcgcgag ccgacgtctt gatcacggcc agtgagtcga gcagcagctg
     4561 cagctcaacc ggaccgctga gcccgcgatc gtgccaggtc acgtcgccac cacgtgcctc
     4621 gtcggtgaac agccggatcg tgaagccttg accggacagg tgggccccga tcgacgcaca
     4681 tgccgagacg gcgtactcga acgacgacgc cggcccggag ccacgatggg cgatgtcgcg
     4741 ggtgtcgagc aggatggttg cccgactctg gtacggctgc tcctcacggc gaaccatcag
     4801 ctcgtcagcc cgcgcggtcg atcgccagtg gatgcgccgc aggtcatcac ccatccggta
     4861 ctcacggacg gtgacgtcct ccgtaccggc cgcggcgaag gctcgcgggc ggttgtcgcc
     4921 ggtgcctgac cagtcgccgg agagctggac ggggggaagt tcgacgacca cgggtgtcac
     4981 gaccagggcc gcccgcgcgg tgaacgcccg ggtcagctcg acgaaaccga acgggtcgct
     5041 caaccggacg gtcagcgggc cgatggtgta gcggccacga acctccgatc gcaccgtgta
     5101 gaccatcgag cgctgccacc gcggacgaag ctggtccagc acgaaacgcg gccggctgcc
     5161 gaggacgtac gggacctggt cctccagcag catcaggccg gtcgggacgc gcccagggtt
     5221 ctgcagatgc agcgcgacgg tggcggtctg tccggcctgg accctggtcg gagtgatctc
     5281 gcgcgcacag gacaacaggt atctcgcccg gttggccagc agcgacgtca cgagcggcaa
     5341 catgacgagc aggatgccga cccgcagcag gtcacgttgg ccgaggatga tcgcacagac
     5401 cgacgccgcg acgccggcag agaagaacgc ccgaccacgg gtcgtgagag tcgaaagggc
     5461 cgagcgcatc gggggctgct tcagtcgtcc actagtagga ctggttcgcg gaggtgcttt
     5521 ccggtacggc gatctggcgg agcagtgcgg tcagcacctg gtccgcgccg cggccgccca
     5581 tctgcgcctc gaggccggtg atcagtcggt gcgccagcac cggtacggcg agcagctgga
     5641 tgtcgtccgg gatcacgtag tcgcgggcct ccagagcggc atgtgcgcgt gccgcgcgta
     5701 cgaggtgaag gctcgcccga ggggaggcac cgagccgcag atcgggtgac cgtcgggtcg
     5761 ccgacgtgat gtcgatcgcg tactgcttca ccggttcgga cacgtgcacg gtgcgaacga
     5821 tgtcgatgag cttggcgatc tcgccgccgt cggtgaccgg ttcgaggtcg tcgagcgggt
     5881 tggcggcgga gtgtgcgtcg agcatgtcga gctcggagcg ctcgacgggg tatcccatcg
     5941 agacgcgagc catgaaccgg tctcgctggg cctccggcag cggataggtc ccctccatct
     6001 cgatcgggtt ctgcgtggcg atgaccatga acggcgcggt cagctcgtac gtcttgctgt
     6061 cgaccgtgac ctgccgctcc tccatgcact ccagcagcgc cgactgggtc ttgggggagg
     6121 cccggttgat ctcgtcgccg acgacgatgt tcgcgaagac gccgccggga cggaactcga
     6181 actccaaggt ctggcggttg tagacggaga caccggtgac atcggacggc agcagatccg
     6241 gcgtgaactg gatccgtttg accgtgcagt cgatcgagcg agcgagggcc ttggccagca
     6301 tcgtcttgcc gacacccggg acgtcctcga tcagcaggtg tccctcggcc aggagaaccg
     6361 tgatggacat gcggatcaca tcacgcttgc cttcgatcac cttctcgatg ttgtcgccga
     6421 cccgactcgt caccgaggcg agggtgtcga aactgctcat ctgttcccct agcggctacc
     6481 caccacgaaa tctccaggtc gcgtgatccg gaggactgca tcttggcaac atcacgcggt
     6541 ggaggcgatg acacaacgat aacgatgtgg aaaccgccac acacgcaggt cttgccgctt
     6601 ccgatcccgc cgctgggtcg g
//
LOCUS       DATBFZ010000263         3384 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_2145205, whole genome shotgun sequence.
ACCESSION   DATBFZ010000263 DATBFZ010000000
VERSION     DATBFZ010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 3384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3384
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2145205"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..897
                     /locus_tag="VH419_08115"
     CDS             <1..897
                     /locus_tag="VH419_08115"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY7043616.1"
                     /translation="GHVKVDGRTIDDVPTYQRNIGFLFQNYALFPHMSVEENIAFPLR
                     ARKISGEQRKRMVDAALELVQLPDTHNRSIRSLSGGQQQRIALARALVFEPSLLLLDE
                     PLAALDKQLREAMQLELKRIQSETGTTTIAVTHDQVEAMSMSDLVAIMNKGRLVQVGT
                     PEEVYARPSDLFVSTFLGEANLIDVDAGAFVGWPAPVKTSAADGVAVVRPEDIELTPA
                     TDGLIDGRVESSVFQGNRRRLTVRCPGLPQPLIVSGASGADAAYGVDAAVGVRLRRNE
                     VHVIPREAADASEVFVEAEVAS"
     gene            1039..1542
                     /locus_tag="VH419_08120"
     CDS             1039..1542
                     /locus_tag="VH419_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020575368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEY7043617.1"
                     /translation="MTRTDTPTAWDERTTLLAFLDYTRQTARAKCEGIAAEHVGAAPL
                     PTSPLTSIGGIVNHMRWVEHSWIENEFVGGPDLGPWTDEEPDKEFSIGAETSIEETLA
                     AYDEQIARTDAIIAGLDLDDRSQAPIRTGERPTLRWVILHLIEENARHNGHLDILREL
                     ADGTTGD"
     gene            1827..2030
                     /locus_tag="VH419_08125"
     CDS             1827..2030
                     /locus_tag="VH419_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017936609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEY7043618.1"
                     /translation="MAQGTVKWFNSEKGFGFITQDDGQPDVFVHFSAIQSDGYRSLEE
                     NAKVEYDVKQGDKGLQAENVRVI"
     gene            2891..>3384
                     /locus_tag="VH419_08130"
     CDS             2891..>3384
                     /locus_tag="VH419_08130"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEY7043619.1"
                     /translation="MSPDRHARTMLPIPDRPPPGLTTYDAKDPETAYPPIEPLLPPEG
                     APNVLVVLLDDVGFGASSTFGGPCRTPTADRLSAGGLRFNRFHTTALCAPTRQALLTG
                     RNHHSVGMGSITETATSAPGNSSLRPNTKAPLPLTLKLNGYSTAQFGKCHEVPVWQSS
                     PLGP"
BASE COUNT          611 a         1149 c         1097 g          527 t
ORIGIN      
        1 ggtcatgtga aggtcgacgg gcgcacgatc gacgacgttc cgacgtacca acgcaacatc
       61 ggcttcctgt tccagaacta cgcgctgttc ccccacatgt cggtggagga gaacatcgcg
      121 ttcccgctcc gggctcgcaa gatctccggc gagcagcgga agcggatggt cgacgcggcc
      181 ctcgaactcg ttcagcttcc tgacacacac aaccgctcga tccgttcgct ctccggcggt
      241 caacagcagc ggatcgcact ggcgcgcgcc cttgtcttcg agcccagcct gctgctgctc
      301 gacgaacccc tggcagctct cgacaagcaa ctccgggaag ccatgcagct ggagctgaag
      361 cggatccaga gcgaaaccgg gacgacgacg atcgcggtca ctcacgacca ggtggaggcg
      421 atgtcgatgt cggatctcgt ggcgatcatg aacaagggtc gcctcgtgca ggtgggcact
      481 cccgaagagg tctacgcccg accgagcgac ctgttcgtct cgaccttcct cggtgaggcg
      541 aacctgatcg atgtcgacgc aggcgcattc gtgggatggc cggcgccggt gaagaccagc
      601 gccgccgacg gcgttgccgt cgtacggccc gaggacatcg aactgacgcc ggcaaccgac
      661 ggcttgatcg acggccgggt cgagtccagc gtcttccagg gcaaccgtcg ccgcctgacc
      721 gtccggtgcc cgggcctgcc ccaaccgttg atcgtgtccg gggcctcggg ggccgacgcg
      781 gcgtacggcg tcgacgcagc tgtcggtgtt cggctgcgac gcaacgaagt ccacgtcatc
      841 ccacgcgagg cggccgacgc ctctgaggtc ttcgtcgaag ccgaggtcgc ctcctgagat
      901 tccagccggc catcccaaac ccctgctgtc caccgcaggt ctgcgatggt tacacgccgc
      961 ttgggctgta aacatcgcaa accacctggc cagaaatgtc tcgacaccgt cggcggccgg
     1021 gcagcagact cagacgccat gacgcgaacg gacactccaa cggcctggga cgagcgcacc
     1081 acgctgctcg cctttctcga ctacacgcgg cagacggctc gcgccaagtg cgaggggatt
     1141 gcggccgagc acgtcggcgc cgcaccctta ccgacctcgc cgctgacgtc catcgggggc
     1201 atcgtcaacc acatgcgatg ggtcgagcat tcgtggatcg agaacgagtt cgtcggtggg
     1261 cccgacctcg ggccatggac ggacgaggag ccggacaagg agttctcgat cggcgccgaa
     1321 acctcgatcg aggagacgct ggcggcgtac gacgagcaga tcgcgcggac cgacgccatc
     1381 atcgccggac tcgatctgga cgaccggtcg caggcgccca tccgcaccgg tgagcgaccg
     1441 actctgcggt gggtgatcct gcacctcatc gaggagaacg cccgccacaa tggtcacctc
     1501 gacatcctgc gcgagctcgc cgacggcacc acgggcgact gagcccgagg cctgctctgg
     1561 acccgtctct gtaggtggtc cgcgacagcg ctgcggccgc gtcaaagccg cggaccgcct
     1621 cgaaggggct cttcgaggca gttcgtgata gcgtcgacgc cagttgcagt gagcagtccc
     1681 ccgcacgttc aaccagcgcc cgtgggcaat cccagtgggc gtctttcgct tttcaaggcc
     1741 cgatgtcttg ctcggcgcag gtgggacgac gttgcaccgc agcacggcgc cgacacaggg
     1801 tcggaccagc agcaaggagc agcacagtgg cacagggaac agtgaagtgg ttcaacagcg
     1861 agaagggctt cgggttcatc acccaggacg acggtcagcc tgacgtcttc gtccacttct
     1921 cggctatcca gtcagacggc taccggtcgt tggaagagaa cgcgaaggtc gagtacgacg
     1981 tcaagcaggg cgacaagggc ctgcaggccg agaacgtccg cgtgatctga caccgccgaa
     2041 gcacaaccaa gggatccgcc gcacctccag gtgcggcgga ttctgcgttt ccaggcagcg
     2101 gaatggcagc agctccgatc cgccacgctc aagagccaac ccacccatga cgaggcagat
     2161 ctggggactt ttgcggtttc tgtgggtgga gatggtcgcg atggatgatc gtggaccccc
     2221 cccagggctc ggtcgtcctc tgctgaggtg ggtcggcagc gaagagttgg aactgctcgc
     2281 gccagctgag ccggtcggcc gacgttccat cccgtttgcc tggtgtgcgt cggtggtggt
     2341 ttgcctgggt ggccatccaa acggacgggt agccagccag acctgccttg ggaggggtgg
     2401 acttgcctgg gcagccatgc aaaccgttgc tcggcgatcg gcgaatgtcc gaaacccgcc
     2461 agtcagagac cgaccacaag gaacggaggt cgggcgaccg gcctcagcgg gcacgaacgt
     2521 gtcccgctcc tcgttgccga cccacctgag ctcgttacga cggagcgccg ggcgaccacg
     2581 cccacccatc acgatcgtct ccgccccaca gaagccggag gagtccccag atctggtggc
     2641 tacccagcca ttcggatggc tacccaggca aacccatcgc tcgactgcag gcgctcagtc
     2701 ggctcaccgg cccaacgctc tcccggacgg cggggcagca cggcgggact gcgagctgat
     2761 cacacgattc ggacgatgcc gaggacggcg ttcgcgcagt acgttggctc gccatggctg
     2821 caggctcgca ctcgccgtcc gaccacggtt ccatcctgtg agccggccga ccgaccgtag
     2881 gaggaacccg atgagcccgg atcgccacgc gcgcacgatg ttgccgatcc ccgaccgtcc
     2941 ccctccaggg ctgacgacat atgacgcgaa ggacccggag accgcgtacc cgccgatcga
     3001 accgttgctg ccgccggagg gagcgcccaa cgtgttggtc gtcctgctcg acgacgtcgg
     3061 gttcggtgcg tcgagtacgt tcggtgggcc gtgtcgtacg ccgaccgcgg accgactgtc
     3121 cgcgggtggg ctgcggttca accgcttcca caccacggcg ttgtgcgcgc cgacccgcca
     3181 ggcactgttg accggccgga accaccactc ggtgggcatg ggcagcatca ccgagaccgc
     3241 cacctcggcc ccgggcaaca gctcgttgcg gccgaacacc aaggccccgc taccgctgac
     3301 cctgaagctc aacggctatt cgacggccca gttcggcaag tgccacgagg tgccggtgtg
     3361 gcagtcatcg ccgctcggac cgtt
//
LOCUS       DATBFZ010000264        16059 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_3129225, whole genome shotgun sequence.
ACCESSION   DATBFZ010000264 DATBFZ010000000
VERSION     DATBFZ010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 16059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16059
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3129225"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="VH419_08135"
     CDS             complement(<1..240)
                     /locus_tag="VH419_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016456662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEY7043620.1"
                     /translation="MKRTFMPRALTTGAVVLTVALGLTGCGASNEGDDDSNGNGGGGS
                     SSLSGQLNGAGATSQEAAMAAWKAGFQGDNSGVTVN"
     gene            complement(474..2423)
                     /locus_tag="VH419_08140"
     CDS             complement(474..2423)
                     /locus_tag="VH419_08140"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011758036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEY7043621.1"
                     /translation="MTTTADQPDYAHVTPLVLPTDDDGWHDWLVSRCDDQLARARSLV
                     ERLRETDRDADARQVLEIWNLVSMALSNAFAASSVMSNVLPNEALRTQAEAAMQEAQK
                     LSTDLTLDAELYATFAALDGADLDDDAQRVLEHTMRDFRRAGVDRDEETRQRLRELAE
                     REVAVGQEFARNIRDDVRRVTFDTAQLAGLPDDYVAAHPVGDDGMIEITTDYPDVYPF
                     LTFAHDRDARREVLLAFNNRAWPANDDLLAELLSLRAEHAALLGYAGWPDYDAEVKMI
                     GTGDAIAGFIDQITAAATASGERDRDRLLARLRIDHPDVERIDRADSMYYAEVLRREE
                     YDVDAQLVRTYFDFSKVRQGLLDVTSRLFGLQWRPRPDVTTWTTDVVTYDVELDDELI
                     GRIYLDLHPREGKFKHAAQFSITAGVNGHQLPEGVLVCNFPRGLMEHREVVTLFHEFG
                     HLVHHVVGGRQDWSRFSGVATEWDFVEAPSQMLEEWAWDAEVLRTFATNAAGEPIPAE
                     LVTKMRAADDFGKGYAARTQMFYAAVSYHLHQQAVPDLTAKVAELQAAYDLFPFIEGT
                     HFHASFGHLHGYTSAYYTYMWILVIAKDLFSAFDRDDLFEATTAARYRDLVLAQGGRR
                     DAADLVEAFLGRPYTIDAFTRWLNQ"
     gene            2471..3277
                     /locus_tag="VH419_08145"
     CDS             2471..3277
                     /locus_tag="VH419_08145"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY7043622.1"
                     /translation="MLASRLGDLSPARRRFVLAVLAVTLVLVGLGIALYVVKRDPGVQ
                     PVSQREPGPVVLVPGYGGSTAGLEVLGDALEQAGRDVSIVRPPGDGTGDLREQAVVLD
                     EAVRAAIERSGASSADVVGYSAGGVVARVWVRDLGGGDLARRVVTLGSPQHGTDVAGL
                     ASDVTPDTCPAACRQLAPDSDLLRRLNAGDETPAGPLWVSIWTQDDTTVVPPESASLD
                     GALDFSVQSVCPSLHVVHGDLPRDPTVIAMTIAELQPSTPELPTQTVCAG"
     gene            complement(3377..4189)
                     /locus_tag="VH419_08150"
     CDS             complement(3377..4189)
                     /locus_tag="VH419_08150"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY7043623.1"
                     /translation="MIAVELRKLLTNRRTWWTIALLDALPTLVAVLLAATDLGPRPGT
                     GPAFLSAVLTDGTLYPLAATAIVLPLFLPAAVAVVAGEAIAGEAQAGTLRYILIRPVG
                     RTRLLVAKLASVMAFVLLAVLIVAGTAYVTGSLLLGDRHGAATASVSGSSLSTEELVW
                     RTGLSLAYAMLSMLAVAAIALFLSTIVESPLAAALGTIGVLIASTLLLTLDAATALYP
                     YLPTRYWLSFVDLFRDPILWRNVERGTALQFVYVAILLAASWANFTTKDITT"
     gene            complement(4186..5094)
                     /locus_tag="VH419_08155"
     CDS             complement(4186..5094)
                     /locus_tag="VH419_08155"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY7043624.1"
                     /translation="MITTTALTKRFGTILAVDQIDLDVREGDIYGFLGANGSGKTTTV
                     RLLLGLVLATSGDIRVLGEPMPAASRSVLPQVGALVEGPAAYRHLSGRANLEVFDAMA
                     PGAGRSTRTGRIDDALDQVGLGGVDERPVRAYSLGMRQRLGLAAALLRTPTLLVLDEP
                     TIGLDPQGIREIRELLVGLQRAGTTIFLSSHLLAEVELMCTRIGVIDRGRLVLQDELA
                     RLQEPTGRMYVRTPDVAAVRALLDGKVEHHDETGLLIRATDAAALNARLVNAGVRVAE
                     LYPERRRLEDVILAATSTGSDRIDRL"
     gene            complement(5091..6185)
                     /locus_tag="VH419_08160"
     CDS             complement(5091..6185)
                     /locus_tag="VH419_08160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043625.1"
                     /translation="MTPARRWCIVAAGVALVIGVPLGLQAVPADDRPLTAAQLLVRIQ
                     ASSNHAYSGYVETLGTLQLPVADRFTDVGSLFGDRTRMRVWWRSDADWRVDKITTTGE
                     TDLYHENSETTTWDYERNEVTLSRDPEVRLPRTADLVPPELTARLLEDVRSDEVTRIG
                     AARVAGVDAIGLRVSPAEPQSSIDHVDIWADPQTGVPLRVQVYADNATSAAFTSEFMS
                     FSPDTPDRARTRFVPPPDSDISYNDVLDIADAANQFASSAPPARLAGLTRSTDTPLRA
                     VGLYGAGVTRLMVVPLWDRAARPLREQLERTPGVRVVEEGDLLAVGPLTVLLTQAESC
                     DAGWLIAGTVTERTVLTAAHDLATSARSLR"
     gene            complement(6246..6461)
                     /locus_tag="VH419_08165"
     CDS             complement(6246..6461)
                     /locus_tag="VH419_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019514768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043626.1"
                     /translation="MRSRSGGWIGIVVIVWLVIGVIAAGQRGYFGDSRDVNCKTASDT
                     ALTIIAGPLNYVGVNPKVDCKVPQPSK"
     gene            complement(6570..7232)
                     /locus_tag="VH419_08170"
     CDS             complement(6570..7232)
                     /locus_tag="VH419_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008362349.1"
                     /note="This protein belongs to the uracil DNA glycosylase
                     superfamily, members of which act in excision repair of
                     DNA. However, it belongs more specifically to UdgX branch,
                     whose founding member was found to bind uracil in DNA
                     (where it does not belong), without cleaving it, appears
                     to promote DNA repair by a pathway involving RecA, rather
                     than base excision; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UdgX family uracil-DNA binding protein"
                     /protein_id="HEY7043627.1"
                     /translation="MPRGRSGADRPGAQEWVPNRLSLAALRRSVQDCRGCELYEGTTQ
                     AVLGAGRADAPLMLVGEQPGDREDVEGKPFVGPAGRLLDKALQAAEIAPELVFRTNAV
                     KHFRFTSGPGKRRMHQSPDLSHMRACAPWLSAELTVVKPRGVVLLGATAGKAIFGSSF
                     RVTIERGHVLSWPDDRAESSPPDWVVATIHPSAVLRADDRDAVLQGLVADLVVAREAL
                     AG"
     gene            7383..8237
                     /locus_tag="VH419_08175"
     CDS             7383..8237
                     /locus_tag="VH419_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043628.1"
                     /translation="MDVAEVSRELYGLPLEEFTATRNARAKQLKADGDADAARRVQAL
                     KKPTLAAWLVNQLVRREPGQIGQLLDLGQQMRAGMSGSTSDDLRQLTKRRYQLVSSLV
                     NAALAATGDRRPGADVATDVQATLEATLSDPDSAQSVQDGCLEAPLHASGFGFVRRPE
                     AEVEEEPGPDADVVDIAAHRERREKALAAARTRVEAAQTAAAEADTKRAESQRLLDKA
                     EAAERKAAAEVRRLETSLERAATALAKRTEAAEQARSELEEAEEAAQDAHDELTDATD
                     AERRLTDR"
     gene            8464..9429
                     /gene="ligD"
                     /locus_tag="VH419_08180"
     CDS             8464..9429
                     /gene="ligD"
                     /locus_tag="VH419_08180"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008356731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEY7043629.1"
                     /translation="MAKASNAVEIDVGPHVVRVTNPDRVYFPQTGATKLDLVEYYLSV
                     GDGIVNALYERPCMMHRFPTGLSGEKVHQKRVPAGAPPWLETVRLHFPRFNRYADELC
                     VTDLAQVIWAVQMSTVEFHPWNSRRADTEKPDEWRIDLDPMPLCDFATVQRTAHVVHE
                     VLDELGAVGWPKTTGGRGMHVYVRIEPAYGFQEVRRAALAFAREIERRMPEDVTTTWW
                     RKDRDPGALFVDYNQNARDHTIACAYSVRGVPDARVSAPVRWDEIDEVTPHDFTIETM
                     PKRFAEIGDLHGGIDNAVFSIEPLLEWADRDERTDPAEPASREDS"
     gene            complement(9501..12146)
                     /locus_tag="VH419_08185"
     CDS             complement(9501..12146)
                     /locus_tag="VH419_08185"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY7043630.1"
                     /translation="MLDLAAAPEAPDGPTERQPTAERSRLVAHLAGFGERTALISAGG
                     SLTYSELASRVEALAVQIGTERRLVLLEAANSVASIVGLLAAWSGRHPVILTAPDRLA
                     ASQSLRSAYAPDVVVTDGADLDGDPVVTHLRTTPGHQLHPDLALLVSTSGSTGSPKLV
                     RLSHRNLLSNAEAIASYLEITPDDRAAMTLPIHYCYGLSVVTSHLLRGSSLVVSDLSV
                     VDRCFWDLFRAAGCTSFAGVPYTFELLDRCGFADLDLPTLRYVTQAGGRLDAATVRRY
                     AGMGRDRGWDLVVMYGQAEATARMAYLPPDRTLDQPEAVGIPIPGGDLRLEPVADTEP
                     DVGELVYSGPNVMMGYARGPSDLALGACVRELHTGDLARRTPDGLYQIVGRRNRFAKL
                     YGHRIDLDQVERLMAANGVAARCVAVDSVLCAFVTSHRRIRKARDLVAHLTGLPPTAT
                     RAAYVQEQPRTANGKVDYHALADYLPTMTDTRSGGGSGSLRDEAARRDGLRNLYAELL
                     GRPDADDHSSFVSLGGDSLSYVEVSIRLGELLPVLPPDWPDRTVAELAADPTPARRGT
                     TVESSVLLRALAIVAIVGTHANLFTYSGGAHLLLAVAGYSFARFQLGNTDRRVRLRRG
                     LAAVAHVAVPSGLIIAGTAAVTGAYTWPTAMFLNGLLGSDSWTDQWRFWFLECLVWTL
                     LACVVVLAIPALHRTERRRPWGFAIVVLAGGIAVRYLLVGVEAGPTQRYTPSIVFGFF
                     ALGWAAQRASSTYQKAVVSAAALVCTLGFFGDLRREGLIVAGVVALVWLPSVRAPAPL
                     ARLLGVVAASSLMIYLTHWQVYPYLEDSHSFTALVLSVLVGVGCKYATDPLLRWVPRL
                     SNRVIEVATTRIRPGVSPTKPATSP"
     gene            complement(12217..13326)
                     /locus_tag="VH419_08190"
     CDS             complement(12217..13326)
                     /locus_tag="VH419_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019510250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY7043631.1"
                     /translation="MTQLEAFGLSKSFGGHPVLTGLDLTVPNRCIAAVLGPSGCGKTT
                     LLRILAGFVDPDAGSVRFGDRTVVSTGRSVPPQHRRVGYVPQEGALFPHLTVGENIGF
                     GLPRSQRRGKRLDSMLDLIELPRAFAHRRPHELSGGQQQRVALARALAAEPAVILLDE
                     PFSSLDAGLREDTGRAVMNALRARHATAVLVTHDQGEALSLADQVAVMRDGRLAQVGA
                     PQALYRAPADPAVARFVGGAALLAAEVKDGIAHCALGELVVTAAGAEQLDDAGQHEVA
                     GPDSRPGAVRQMDVLVRPEQIRITDSGNEAAGVPAVVAEVSFYGHDAAVRLDVVPDGP
                     RVVARVLGDETPVTGAVVRLAVHGPVTAFPATPGG"
     gene            complement(13323..14882)
                     /locus_tag="VH419_08195"
     CDS             complement(13323..14882)
                     /locus_tag="VH419_08195"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEY7043632.1"
                     /translation="MSIALRSRPATTAAPRPPAALLLAGTLVAALALVPLGFVVVYTI
                     SVGPSEAWQLLGRPRIAMLLSNTLKLVLGGVLLSLVVGIAAAWFVERTRLPLPRVWHV
                     LLCAPLAVPSFVNGFGWVSLTHSVQTYPGVLMVVTLSYFPLVYLPVAAVLRGLDPALE
                     ETAYSLGRGVWRTFFTVILPQLRPAALGGGLLVGLHLLAEFGAVQMLRFPTFTTAIYD
                     QYRSSFNGPAANMTASVLVLCCLVLLVGELRLRGSDRYARVGRGTARRAVRRRLGILT
                     VPVVLGLTGLLALALGVPAYSLIHWLSVGSSTAFPVSDLVSTAATSIGLGLVAAFVTT
                     LFALPVAWLAVRFPRRSSVVVERSSYVANALPGIVVALAFITVSIRVVEPLYQTLVLL
                     IAAYSVMFLPRAVVSVRAALEQAPPVLDDVSRSLGAGQLETARRVTVPLILPGVGAGA
                     ALVFLATVTELTATLLLAPIGTQTLATQFWSASSSLAYGAAAPYALLMVLISLPATFL
                     LSAQVGRTRRA"
     gene            complement(14890..15903)
                     /locus_tag="VH419_08200"
     CDS             complement(14890..15903)
                     /locus_tag="VH419_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008361583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HEY7043633.1"
                     /translation="MRTIVRGLAAVLAAGAVLTTLTACSGDSGPSITVYNAQHEELLK
                     ALAPKFTAETGIDVHLRNGEDFELGNQLVQEGSSSPADVFLTENSPAMSLVDSKGLFA
                     PLEQNTLNSIPAQYRPDDGNWTGFAARSTVLVYNTDAVSKAELPRSIMDLADPEWAGR
                     VAFSPTGADFQAIVSAVLQTQGEQATQQWLDGLAKNGEVYDGNNLVLEAVNSGEVDVG
                     IIYHYYWYRDQAESGENSDDSALYFFGNQDPGAFVSVSGAGVLKSSDHQADAQKFVAF
                     LVSREGQQALADSYALEYPLNPHVQLEQGVKPFDSLQPPTIDMTSLNGPKVIELMQNA
                     GLL"
BASE COUNT         2614 a         5382 c         5727 g         2336 t
ORIGIN      
        1 gttgaccgtg acgccggaat tgtcgccctg gaagccggcc ttccaggcag ccatggcggc
       61 ctcctgcgac gtggcgccgg cgccgttgag ctgaccagac agcgatgagg aaccgccgcc
      121 gccgtttccg ttgctgtcgt cgtcgccctc gttgctggcg ccgcagccgg tcagcccgag
      181 cgccaccgtc aggacgacgg cgccggtggt cagtgcgcgt ggcatgaatg tgcgcttcaa
      241 gtcaggtccc ttctgggatc gtgcgatgag tggtgttgtc gactgcgacg ctagggatca
      301 gaggtgtccg aaccggtggc cgctggtgaa cggggagtga acgcttgtca gccgttcaac
      361 ttccgttggg cgtgctggag acggacgtca catgaacggc cgggtggtcg cccatgcgcc
      421 taccaccgga ccgccgccgg ggttcgggag tggaggagcg agcggacggc cggctactgg
      481 ttgagccagc gggtgaaggc gtcgatcgtg tacgggcgac cgaggaaggc ttcgaccaag
      541 tcggctgcgt cgcgcctgcc accttgggcg agcacgaggt ctcgataccg ggcagctgtc
      601 gttgcctcga acaggtcatc gcgatcgaac gcggagaaca gatccttggc gatcaccagg
      661 atccacatgt acgtgtagta cgccgacgtg tagccgtgca ggtgaccgaa cgacgcgtgg
      721 aagtgcgtcc cttcgatgaa cgggaacagg tcgtacgccg cctggagctc ggccaccttc
      781 gccgtcaggt ccgggacggc ctgctggtgc aggtggtacg acacggccgc gtagaacatc
      841 tgcgtgcgag cggcgtaccc cttgccgaag tcgtcggccg cacgcatctt cgtcaccagc
      901 tcggcgggga tcggctcacc ggcggcattc gtggcgaacg tccggaggac ctccgcgtcc
      961 cacgcccact cctccagcat ctgcgacggt gcctcgacga agtcccactc ggttgccacg
     1021 ccggagaacc gcgaccagtc ctgtcggccg ccgacgacat ggtggacgag gtggccgaac
     1081 tcgtggaaga gcgtcacgac ctcgcgatgc tccatgaggc cgcgcgggaa gttgcacacg
     1141 agcacccctt caggcagctg gtgcccgttg accccggcag tgatgctgaa ctgcgccgcg
     1201 tgcttgaact tgccctccct tgggtgcagg tccaggtaga tgcgaccgat cagttcgtcg
     1261 tcgagctcga cgtcgtacgt cacgacgtcc gtggtccacg tggtcacgtc gggccgcggt
     1321 cgccactgga gtccgaagag cctgctggtc acgtcgagca ggccctgccg gaccttggag
     1381 aagtcgaaat acgtccgcac gagctgcgcg tcgacgtcgt actcctcacg gcgcagcacc
     1441 tcggcgtagt acatgctgtc ggcgcgatcg atgcgctcga cgtcagggtg gtcgatccgc
     1501 aaccgggcga gcagccggtc acgatcgcgc tcacccgacg cggtggccgc ggctgtgatc
     1561 tggtcgatga aacccgcgat ggcgtcgccg gtgccgatca tcttgacctc ggcgtcgtag
     1621 tccggccagc cggcgtaccc gagcagcgct gcatgctcgg cccggagcga gagcagctct
     1681 gcgagcaggt cgtcgttggc cggccaggcg cggttgttga aggcgagcag cacctcgcgc
     1741 cgggcgtccc ggtcgtgcgc gaacgtcagg aacggataga cgtccgggta gtcggtcgtg
     1801 atctcgatca tgccgtcgtc cccgaccggg tgcgccgcca cgtagtcgtc cggcagtccc
     1861 gcgagctgcg cggtatcgaa cgtgacccgg cgtacgtcgt cacggatgtt cctggcgaac
     1921 tcctggccaa ccgccacctc gcgttcggcc agctcgcgca gtcgctgacg ggtctcctcg
     1981 tcgcggtcga cgccggcccg gcgaaagtcg cgcatcgtgt gctcgaggac ccgctgtgcg
     2041 tcgtcgtcca ggtcggcgcc gtccaacgcg gcgaacgttg cgtagagctc cgcgtccagc
     2101 gtgaggtcgg tcgagagctt ctgcgcctcc tgcatggcgg cttccgcctg agtccgaagc
     2161 gcctcgttcg gtaacacgtt gctcatcacc gacgacgccg cgaatgcgtt ggacagcgcc
     2221 atcgacacga ggttccagat ctccagcacc tgacgggcgt ccgcatcgcg gtcggtctcc
     2281 cggagccgtt cgacgagtga cctggcgcgg gcgagctggt cgtcacagcg cgacacgagc
     2341 cagtcgtgcc agccgtcgtc gtcggtcgga agcacgagcg gggtcacgtg tgcgtagtcg
     2401 ggctggtctg cggtggtggt cacccgagca cactagatga acggttggcc tggcgaggga
     2461 ggatggcggg gtgctggctt ctcggctggg tgacctgtcg cctgctcgtc ggcggttcgt
     2521 gctggccgtc ctcgccgtga ccctcgtgct ggtcggactc ggcatcgcac tgtacgtcgt
     2581 caagcgtgat cccggcgtac aacccgtgtc acagcgagag cctgggccgg ttgtgctcgt
     2641 ccccgggtac ggcggctcga ccgcggggct ggaggtcctg ggcgacgcac tcgaacaggc
     2701 tggccgtgac gtatccatcg tgcggccgcc cggcgacgga accggcgacc tgcgtgagca
     2761 ggcggtcgtg ctcgacgagg ccgtccgggc ggcgatcgag cggtcggggg cctcgtcggc
     2821 ggatgtggtc ggctactcgg ccggtggcgt cgtggcccgg gtctgggtgc gtgacctggg
     2881 cggtggggat ctcgctcgcc gcgtcgtgac actggggtcg ccgcagcacg gcaccgacgt
     2941 agccggactg gccagcgacg tcacgccgga cacctgtccg gcggcgtgtc ggcagcttgc
     3001 gccggacagc gacctgctac gtcggctcaa tgcgggcgac gagacgcctg ccggtccgct
     3061 ctgggtgtcg atctggaccc aggacgacac gaccgtcgta ccgccggagt cggcatcgct
     3121 cgacggggca ctcgacttct ctgttcagtc ggtctgcccg tccctccacg tggtgcacgg
     3181 cgacctccct cgggatccga cggtcatcgc catgacgatc gccgagctcc agccgtcaac
     3241 cccggagctt cccactcaga ccgtctgcgc cggctgaccc aactgccggc ctcccgcgaa
     3301 ttgcgggaaa gtgcacgccg ccacgccgac cgggaccgcc ttttccccgc aattcgcggt
     3361 gacaaacggc ggatggtcag gtcgtgatgt ctttcgtggt gaagttcgcc cagctggccg
     3421 ccagcaggat cgcgacgtac acgaactgca gcgctgttcc gcgttcgacg ttgcgccaca
     3481 ggatcggatc gcggaagagg tcgacgaacg acagccagta gcgggtcggc aggtaggggt
     3541 acagcgcggt cgccgcgtcc aacgtgagga gcagggtcga cgcgatgagg accccgatcg
     3601 tgccgagcgc agccgcgagc ggcgactcga cgatggtcga gaggaacagc gcaatcgcgg
     3661 cgacggcgag catggagagc atggcgtagg cgagcgacag acccgtccgc cagaccagct
     3721 cctccgtcga gagcgagctg cccgagacac tggccgttgc cgcaccgtgc ctgtcgccca
     3781 gcaggaggga gccggtgacg tacgccgttc cggccacgat cagcacagcc agcagcacga
     3841 atgccatcac gctcgccagc ttcgccacga gcagtcgagt ccggccgacc ggtcggatca
     3901 gtatgtagcg cagcgtcccc gcctgggcct ctccggcgat cgcctcgccg gccacgacag
     3961 cgaccgcggc aggaaggaac agcggcagca cgatcgccgt cgccgccagc ggatagagcg
     4021 tcccgtcggt caacaccgcg gagaggaagg ccggtccggt gcccggccgc gggccgaggt
     4081 cggtggccgc gagcagcaca gccaccaatg tgggaagcgc gtcgaggagc gcgatcgtcc
     4141 accacgtacg ccggttggtc agcagcttgc gcagctcgac ggcgatcaca gtcggtcgat
     4201 ccgatcggag ccggtgctcg tggccgcgag gatgacgtcc tcgagccggc gacgctcggg
     4261 gtagagctcc gccacacgta cgccggcgtt gaccagccgg gcgttgaggg cggctgcgtc
     4321 ggttgccctg atcaggagcc ctgtctcgtc gtggtgctcg accttgccgt cgagcagcgc
     4381 acgcacggcg gcgacgtcgg gcgtccggac gtacatccga ccggtgggtt cctggaggcg
     4441 agccagctcg tcctgaagca ccaaccggcc cctgtcgatg acgccgatcc tcgtgcacat
     4501 cagctccacc tcggcgagca ggtggctcga caggaagatc gtcgtcccgg cccgctgcag
     4561 cccgaccagc aactcgcgga tttcccggat cccctgcggg tccaggccga tcgtcggctc
     4621 gtccagcacg agcagcgtcg gtgttcgcaa cagcgcggcc gcgagcccga gccgctgccg
     4681 catccccagc gagtacgcgc gcaccggccg ttcgtcgaca cctccgagcc cgacctggtc
     4741 gagcgcgtcg tcgatgcggc cggtgcgggt ggaccggcct gctcccggtg ccatcgcgtc
     4801 gaacacctcc aggttcgccc gaccggagag gtgacggtat gccgctggac cctcgaccag
     4861 cgcaccgacc tgcggcagca ccgaccgcga ggcggcaggc atcggctcac cgagaacacg
     4921 aatgtcgccg gaggtggcca gcaccaggcc cagcaacagt ctcaccgtcg tggtcttgcc
     4981 ggatccgttg gcgccgagga agccgtagat gtcgccctcg cgcacgtcca ggtcgatctg
     5041 gtcgaccgcg aggatggtgc cgaaccgctt ggtcagtgcg gtggtcgtga tcatcgcagg
     5101 gaccgcgcgg aggtggccag gtcgtgcgct gccgtcagga ccgtgcgttc cgtcaccgtg
     5161 ccggcgatca gccagccggc atcacacgac tcggcctgcg tgaggaggac cgtgagtggt
     5221 ccgaccgcga gcaggtcgcc ctcctcgacg acccggacac caggtgttcg ctcgagctgc
     5281 tcgcggagcg gtcgcgccgc ccggtcccac agcgggacca ccatcagtcg ggtgacccct
     5341 gcgccgtaca ggccgacggc tcgcaacggc gtgtccgtcg accgcgtcag cccggccagt
     5401 ctcgcgggcg gtgcgctgga cgcgaactgg ttggcggcgt cggcgatgtc gaggacgtcg
     5461 ttgtaggaga tgtcggaatc cggcggcggc acgaagcggg tgcgtgcgcg atccggcgtg
     5521 tcgggggaga aggacatgaa ctcggacgtg aacgccgcac tcgtggcgtt gtcggcgtac
     5581 acctgcacgc gcagcgggac gccggtctgc gggtctgccc agatgtcgac gtggtcgatg
     5641 ctggactgtg gttccgcggg cgagacacga agcccgattg cgtcgacacc cgcaacgcga
     5701 gctgctccga tccgggtgac ctcgtcgctg cgaacgtcct ccagcaaccg tgcggtcagt
     5761 tcgggcggca cgaggtctgc ggtgcgggga agccggacct ctgggtcgcg gctcagggtg
     5821 acctcgttcc gctcgtagtc ccacgtcgtc gtctcgctgt tctcgtggta caggtcggtc
     5881 tcgccggtcg tggtgatctt gtccacgcgc cagtcggcgt cgctgcgcca ccacacgcgc
     5941 atccgggtcc ggtcaccgaa cagcgagccg acgtcggtga agcggtccgc gacgggcagc
     6001 tgcaacgtac ccaacgtctc gacgtagccg gagtacgcgt ggttgctgct cgcctggatg
     6061 cgcaccagca gctgagcggc cgtgagaggt cgatcatccg cagggacagc ctgcagtccc
     6121 agcggaactc cgatcaccag agcgacgccg gccgcgacga tgcaccaccg gcgggcgggg
     6181 gtcatgcctc agcctacgtc cgccttcccc gcgtcctcat gtgtacggaa gggcaggttg
     6241 gccgatcact tggagggttg agggaccttg cagtcgacct tcgggttgac gccgacgtag
     6301 ttgagaggac cggcgatgat cgtcaacgcc gtgtcgctcg cggtcttgca gttcacgtcc
     6361 cgcgagtcgc cgaagtatcc acgttgcccg gcagcgatca ctccgatcac caaccacacg
     6421 atgacgacga tgccgatcca gccgcccgac cgtgacctca tggtgcctcc tgtcgccggg
     6481 ccgcatgccg cgcgcccgag gtggtcagac tcgcaatacc cacgtcaagc cgcgcctatc
     6541 gcagcgcgcc ccgaagggcc gggctcggct caaccggcca gggcctcgcg cgccaccacg
     6601 aggtcggcga ccaggccttg cagtacggcg tcccggtcgt ccgcgcgcaa caccgccgac
     6661 ggatggatcg tggcgacaac ccagtccggc ggagacgact ccgcccggtc gtcgggccac
     6721 gacagcacat ggccgcgttc gatcgtgaca cggaacgatg aaccgaagat ggcctttccg
     6781 gcggtggcgc cgagcaggac cacgccgcga ggcttcacga cggtcagctc cgctgacagc
     6841 cacggcgcac acgcacgcat gtgtgacagg tccggtgact ggtgcatgcg gcgcttgcca
     6901 ggaccactgg tgaagcggaa gtgcttcacc gcgttggtgc ggaacaccag ttcgggggcg
     6961 atctcggccg cctgcagagc cttgtcgagc agtcgcccgg ccggtccgac gaacggcttg
     7021 ccttccacgt cctcgcggtc tcccggttgc tcgccgacga gcatgagcgg agcgtcggcc
     7081 cgtcccgcac cgagcaccgc ctgggtcgtt ccttcgtaga gctcgcagcc tcggcagtcc
     7141 tgcacggatc ggcgcagcgc ggccagtgag agccggttcg ggacccactc ctgtgcgccg
     7201 ggccggtccg cgccgctgcg gccgcgtggc atggatgctc ctcgagtgcg gtaggtggac
     7261 tgggagagcc gtacccgagc caacctcgct gcaaacggcg tgcccgccgg gcgacgggtc
     7321 tcgacgggct ggacctccgg gtggctgggg ggtgggggac atgagtacga aagactggcg
     7381 gggtggacgt ggctgaggtg tcgagggagc tgtacgggct cccgctcgag gagttcaccg
     7441 cgacccgcaa cgcccgtgcc aagcagctca aggccgatgg ggacgcggac gccgctcgcc
     7501 gggtccaggc gctgaagaag ccgacgctcg ccgcctggct ggtgaatcaa ctggtacgcc
     7561 gtgagcctgg gcagatcgga cagctcctcg acctcgggca gcagatgcgg gccgggatgt
     7621 cggggtcgac cagcgacgac ctgcgtcaac tgacgaagcg gcgctaccag ctggtgtcga
     7681 gtctggtcaa cgctgcgctc gcggccaccg gtgaccgtcg gcccggggcg gacgtcgcga
     7741 ccgacgtgca ggcgaccctg gaggcaacgc tgtccgaccc ggacagtgcg cagtcggtgc
     7801 aggacggctg cctggaggct cctctgcacg cgtccgggtt cgggttcgtc cgtcgtcccg
     7861 aggccgaggt ggaagaggag ccgggaccgg acgccgacgt cgtcgacatc gcggcgcacc
     7921 gggagcgccg ggagaaggcg ttggcggcgg cacgcacccg agtcgaggcg gcgcagacgg
     7981 cggcggccga ggccgacacg aaacgcgctg agtcccagcg tctgttggac aaggccgagg
     8041 cagccgagcg caaggcggca gcggaggtac gccggctgga gacctcactc gaacgcgcgg
     8101 cgaccgctct ggccaagcgg accgaagcgg cggaacaggc gaggtcggag ctggaggagg
     8161 ccgaggaagc cgcccaggac gcgcacgacg agttgacgga cgcgacggat gccgagcgcc
     8221 gcctcaccga ccgctgacgt cggcgggtat cagtagtcga cgtggtgcct ccttgtgcct
     8281 cagcgaccaa ggagggcgtc atgcttccac cacaggtggg ttccgttctg atgcggctgg
     8341 gccgcaagcg tggtcatgcg gacgagcgtc gtcgtccgac gcgacccgtc gtacgccggc
     8401 cggcagtgcc acaacagcgt tcgggatcgc cactctcctg acgcttgcgg ctacgttggc
     8461 cgcatggcga aggcgtcgaa cgcggtcgag atcgacgtcg gaccgcacgt cgtccgcgtc
     8521 accaaccccg accgggtgta cttcccgcag accggcgcca cgaaactcga tctcgtcgag
     8581 tactacctca gtgtcggcga tggcatcgtc aacgcgctct acgagcggcc gtgcatgatg
     8641 caccggttcc caaccgggtt gagcggcgaa aaggtccacc agaagcgcgt tcccgccgga
     8701 gcgcctccct ggctggagac ggtgcggctg cacttcccac ggttcaaccg gtacgccgac
     8761 gagctgtgtg tgacggacct ggcgcaggtg atctgggcgg tgcagatgtc gacggtcgag
     8821 ttccacccct ggaactctcg ccgcgccgac accgagaagc cggacgagtg gcggatcgac
     8881 ctcgacccga tgccgctgtg cgactttgcc accgtgcagc ggacggccca tgtggtgcac
     8941 gaggtgctcg acgagctggg tgccgtcggc tggccgaaga ccaccggtgg gcgggggatg
     9001 cacgtctacg tgcggatcga gccggcgtac gggttccagg aggtacgccg cgcagcgctg
     9061 gcgttcgccc gcgagatcga acgacggatg cccgaggacg tgaccaccac gtggtggcgc
     9121 aaggaccgtg acccgggtgc gctgttcgtc gactacaacc agaacgcgcg tgaccacacg
     9181 atcgcgtgcg cgtactccgt tcgcggcgtg ccggacgcga gagtgtcggc gccggtgcgc
     9241 tgggacgaga tcgacgaggt cacgccgcac gacttcacga tcgagaccat gccgaagcgg
     9301 ttcgctgaga tcggtgacct ccacggcggc atcgacaacg cggtcttctc catcgagccc
     9361 ctgctcgaat gggccgaccg agacgaacga accgaccccg ccgaaccggc atcccgtgag
     9421 gactcctgac ctccatcgag gtggcactgc cgggtcgaca cgccgtccaa atccaacgaa
     9481 acctgccacc tcgccgagga ctacggcgag gtggcaggtt tcgttggaga cacgccgggt
     9541 cggatgcgcg tggttgccac ctcgatcacg cggttggaga gacgcgggac ccagcgcagc
     9601 aacgggtcgg tcgcgtactt gcagccgacg ccgacgagca cggacagcac gagcgcggtg
     9661 aacgagtggg agtcctcgag atacggatag acctgccagt gcgtcaggta gatcatcagc
     9721 gagctcgcgg cgacgacacc gaggagacgg gcgagcggcg ccggtgcgcg taccgaaggc
     9781 agccagacca gcgcgaccac gccggcgacg atcagtcctt cacggcgcag gtcaccgaag
     9841 aacccgagcg tgcagacgag tgccgccgcg ctgacgacgg ccttctggta cgtcgacgag
     9901 gctcgctgcg ccgcccagcc caacgcgaag aagccgaaca cgatcgacgg cgtgtagcgc
     9961 tgggtcggac cggcttcgac gccgacgagc aggtagcgca ctgcgatgcc accggcgagc
    10021 acgacgatcg cgaagcccca cggccttcgg cgctccgtac ggtggagggc cgggatcgcg
    10081 agcacgacga cacaggccag cagcgtccac accagacact cgaggaacca gaagcgccac
    10141 tggtcggtcc acgagtcgct gccgagcagc ccgttgagga acatcgctgt cggccacgtg
    10201 tacgccccgg tgaccgcggc tgtcccggcg atgatcaggc cactcgggac cgccacatgg
    10261 gccacggccg ccaggcctcg ccgcagccgg acacggcggt ctgtgttgcc gagctggaag
    10321 cgcgcgaagc tgtagcccgc caccgccagc agcaggtgtg cgcccccgga gtaggtgaac
    10381 aggttcgcgt gtgttccgac gatcgcgacg atcgccagcg cccggagcag cacggaactc
    10441 tcgacggtcg tgccgcgcct ggcaggcgtc gggtctgcgg cgagctcggc cacggtccga
    10501 tcaggccagt cgggcggcag cacgggcagc agctcaccga gccggatcga cacctcgacg
    10561 tacgacagcg agtcaccacc gaggctgacg aacgagctgt ggtcatcggc atccgggcgg
    10621 cccagcagct cggcgtacag gttccggaga ccgtcccggc gtgcggcctc gtcgcggagc
    10681 gagccggagc ctccgcccga cctcgtgtcg gtcatcgtcg gcaagtagtc ggccagggcg
    10741 tggtagtcga ccttgccgtt cgccgtccgc ggctgctcct gcacgtacgc cgcccgggtg
    10801 gcagtgggcg gcagacccgt caggtgggcc accaggtctc gggccttccg gatccttcga
    10861 tggctggtga cgaacgcgca gagcacgctg tcgaccgcga cacagcgggc agccacgccg
    10921 ttggccgcca tgaggcgctc gacctggtcg aggtcgatcc ggtggccgta cagcttggcg
    10981 aaccggttcc ggcggccgac gatctggtag aggccgtccg gcgtacgacg ggcaaggtct
    11041 cccgtgtgca gctcccgcac gcacgcgccg agcgcgaggt cggaaggtcc gcgggcgtag
    11101 cccatcatca cgttggggcc ggagtagacg agctcgccga cgtcgggctc ggtgtctgcg
    11161 acgggctcga gtcggaggtc accgccaggt atcgggatgc cgaccgcctc gggttggtcc
    11221 agggtgcgat ccggcggcag gtacgccatc cgggcggtcg cctcggcctg cccgtacatc
    11281 accacgaggt cccagccgcg gtcacggccc atgccggcgt accgccgcac ggtcgcagcg
    11341 tcgagccgcc cgccggcctg ggtgacgtag cgaagcgtcg gcaggtcgag gtccgcgaag
    11401 ccgcagcggt cgagcagctc gaacgtgtac gggacgcccg cgaacgacgt gcaaccggcg
    11461 gcccggaaca ggtcccagaa gcagcggtcg acaacggaca ggtcgctgac gacgagcgac
    11521 gaaccccgga gcaggtggct cgtcacgaca gaaaggccgt agcagtaatg gatcggcaac
    11581 gtcatggccg cacggtcgtc aggcgtgatc tccaggtatg acgcgatggc ttcggcgttg
    11641 ctcagcaggt tccggtgaga aagccggacc agcttcggcg acccggtcga gccggaggtg
    11701 ctgaccagca aggcgaggtc agggtgcagc tgatggccgg gggtcgtgcg gaggtgggtg
    11761 acgacgggat cgccgtcgag gtcggcgccg tccgtgacga cgacgtcggg agcgtacgcg
    11821 gaccgcaggg actgactcgc cgccagccgg tccggagcgg tcaggatcac ggggtgccga
    11881 ccggaccagg cggcgagcag ccccacgatc gacgcgacgg agttggccgc ctcgagcagc
    11941 accagccgtc gttcggtgcc gatctgcacg gcgagggcct cgacccggct cgccagctcg
    12001 ctgtacgtga gagagcctcc ggcgctgatc agcgcagtgc gttcgccgaa acccgccagg
    12061 tgcgcgacga gccgcgaccg ctcagccgtc ggctgccgct cggtcggacc gtccggtgcc
    12121 tccggggcgg cggcgaggtc gagcatgcgc gggagtgtag tttggtaagg cttacctaag
    12181 caacggtgga tccgaccatc gctcgtccgg agcggctcag ccgccgggcg ttgccgggaa
    12241 tgcggtcacc gggccgtgca cggccagccg aacgacggcc ccggtgaccg gtgtctcgtc
    12301 accaagcacc cgagccacga cacgcggccc gtccgggacc acgtcgagcc ggacggcggc
    12361 gtcgtggccg tagaagctga cctcggcgac gactgccggc acgccggccg cctcgttgcc
    12421 gctgtcggtg atccggatct gttctggtcg gaccagcacg tccatctgcc ggacggcgcc
    12481 gggccggctg tccggaccag ccacctcgtg ctgtcccgcg tcgtccagct gctcggcgcc
    12541 ggctgctgtc acgacgagct cgccgagggc gcagtgcgcg attccgtcct tcacctcagc
    12601 cgccagcagc gcggccccac cgacgaagcg ggcaacggcc gggtcggccg gggcgcggta
    12661 gagggcctgg ggcgcaccga cctgggcgag ccggccgtcc cgcatcaccg ccacctggtc
    12721 ggccaacgag agcgcttcac cctggtcgtg cgtgacgagc acggcggtgg cgtggcgggc
    12781 acgcagggcg ttcatgaccg cacgtccggt gtcctcacgc agaccggcat cgagcgacga
    12841 gaagggctcg tcgagcagga tcaccgcagg ctcggccgcc agcgcgcggg cgagtgcgac
    12901 ccgttgctgc tgcccgccgg agagctcgtg cgggcgccga tgggcgaacg cgcggggcag
    12961 ctcgatcagg tcgagcatgc tgtcgagccg tttgccacgt cgctgcgacc gcggcagtcc
    13021 gaagccgatg ttctcgccga cggtgaggtg cgggaagagc gcgccctcct gtggcacgta
    13081 acccacccgc cggtgctggg gtggcaccga ccggccggta ctcacgacgg tccggtcgcc
    13141 gaacctcacc gaacccgcat cggggtcgac gaagccggcg aggatgcgca gcaacgtggt
    13201 cttcccgcac ccggagggac cgagcacggc ggcgatgcac cggttcggga cggtcaggtc
    13261 cagcccggtc aggacgggat gtccgccgaa gctcttgctg agcccgaagg cctcgagctg
    13321 ggtcatgcgc gtctcgttct tccgacctgg gcgctgagca ggaacgtcgc gggcagcgag
    13381 atcagcacca tcaacaatgc gtacggcgca gcggcaccat acgccaatga tgacgacgcc
    13441 gaccagaact gcgtggcgag ggtctgcgtg ccgatcggcg cgagcagcag ggtcgcggtc
    13501 agctcggtga ccgtcgcgag gaacaccagt gcggctcctg caccgacacc gggcaggatc
    13561 agcggcacgg tgacccggcg ggcggtctcc agctggccgg cgccgaggct gcgtgacacg
    13621 tcgtcgagga cgggtggcgc ctgctcgaga gccgcccgca cgctgacaac ggcccgcgga
    13681 aggaacatga cggagtacgc cgcgatcaac agcaccaggg tctggtacag cggctcgacg
    13741 acccggatcg acaccgtgat gaaggcgagt gcgacgacga tccccggcaa cgcgttggcg
    13801 acataggagc tgcgctcgac cacgacgctg ctgcgtcgcg ggaaccgcac cgccagccag
    13861 gccacgggga gagcgaagag cgtggtgacg aacgcggcga ccaggccgag accgatcgac
    13921 gtggcggccg tcgagacgag gtcgctgacg ggaaaggcgg tcgacgagcc gacgctgagc
    13981 cagtggatga ggctgtacgc cggtacgccc aaggcgaggg cgagcagccc ggtcaacccg
    14041 agcacgaccg gcacggtgag gatgccgagc cggcgccgga cggctcggcg agcggtgccg
    14101 cgcccgacgc gggcgtaccg gtcggagcca cgcagccgca gctcgccgac gagcagcacc
    14161 aggcagcaga gcacgaggac gctcgcggtc atgttcgccg ccggaccgtt gaacgatgac
    14221 cggtactggt cgtagatggc ggtcgtgaac gtcgggaagc gcagcatctg cacggcgccg
    14281 aactccgcga gcagatgcag tccgaccagc agaccgcctc cgagtgccgc cggtcgcagc
    14341 tgcggaagga tgacggtgaa gaacgtgcgc cagacaccgc gtccgagcga gtaggccgtc
    14401 tcctccagcg ccgggtcgag gccgcggagt acggccgcca ccggcaggta gaccagcggg
    14461 aagtacgaca gggtcacgac catcagcacg ccggggtagg tctgcacgct gtgcgtgagc
    14521 gagacccagc cgaacccgtt gacgaaggac ggcacggcca gtggggcgca cagcaggacg
    14581 tgccacacac gcggcaacgg cagtctcgtc cgctcgacga accaggcggc ggcgatcccg
    14641 accacgagcg acaacagcac gccgccgagc accagcttca gcgtgttgga cagcagcatc
    14701 gcgatccgtg gccgcccgag cagctgccag gcttcggacg ggccgaccga gatggtgtag
    14761 acgaccacga acccgagcgg aaccagcgcc aacgcggcca ccagggtgcc ggcgagcagc
    14821 aacgccgccg gcggccgcgg cgctgccgtg gtcgccggcc gggaacgaag ggcgatactc
    14881 atcggctgct cagaggagac cagcgttctg catgagctcg atgaccttcg gaccgttcag
    14941 gctggtcatg tcgatggtcg gcggctggag cgagtcgaac ggcttgacgc cctgctcgag
    15001 ctgcacgtgc gggttgagcg ggtactcgag cgcatagctg tcggccagcg cctgctgacc
    15061 ttctctgctg acgaggaagg ccacgaactt ctgcgcatcg gcctgatggt cgctcgactt
    15121 cagcacgccg gccccggaga cgctgacgaa cgcgcccggg tcctggttgc cgaagaagta
    15181 caaggccgag tcgtcgctgt tctcaccgga ctcggcctgg tcgcggtacc agtagtagtg
    15241 gtagatgatc cccacatcga cctcgccgga gttgaccgct tcgaggacga gattgttgcc
    15301 gtcgtacacc tcaccgttct tggcgagacc gtccagccac tgttgagtcg cctgctcgcc
    15361 ctgcgtctgc aagacggccg acacgatcgc ctggaagtcg gcgccggtcg gtgagaacgc
    15421 gacccggcct gcccactccg ggtcggcaag gtccatgatc gacctcggca gctcggcctt
    15481 gctgacggcg tcggtgttgt agacgaggac ggtcgaccgg gcggcgaagc cggtccagtt
    15541 gccgtcgtca gggcggtact gcgccgggat cgagttgagt gtgttctgct cgagcggagc
    15601 gaacagtccc ttgctgtcga cgagcgacat ggcgggggag ttctcggtca ggaacacgtc
    15661 cgccggtgac gaagacccct cctgcacgag ctggttgccg agctcgaagt cctcgccgtt
    15721 gcgcaggtgg acgtcgatgc cggtctcggc ggtgaacttg ggcgccaacg ccttcagcaa
    15781 ctcctcgtgc tgagcgttgt acacggtgat cgacggaccc gagtcgccgc tgcacgcagt
    15841 gagggtggtg aggacggctc ccgccgccag cactgccgcc aagccgcgga cgatcgttcg
    15901 catgcggttc ctcttcgccg gatatgagcc tgggcatgag ccctgggtat gagccaggtc
    15961 acaattaggg ttgccttacc ttagaagagg ttagcccagc atgctccgca cgcgcgatac
    16021 agcccggccc accgacgagg tccacgagtg cgggagtct
//
LOCUS       DATBFZ010000265         7742 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_3130246, whole genome shotgun sequence.
ACCESSION   DATBFZ010000265 DATBFZ010000000
VERSION     DATBFZ010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 7742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7742
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3130246"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(565..1296)
                     /locus_tag="VH419_08205"
     CDS             complement(565..1296)
                     /locus_tag="VH419_08205"
                     /inference="COORDINATES: protein motif:HMM:NF025343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY7043634.1"
                     /translation="MAGRRTELAVQAEGDGIDGLGKTEAVDIVDQRREQMLAAAAELI
                     VERGFSETRIADVARRIGTSPALVIYYFGTKDDLLTAALRHSEDRFYTACAEMLASTP
                     ALRDRLEKLVRLTCVPEGENEMSGAWGLWFDLWAQAFRHPLVATDRVELERRWRSTIA
                     GVVADGIEAGEIEPVDADDFAVTWAVLLDGLSIQVALNDPIVDPNRAFEIAMSFAERA
                     LALPPKRRPAKKTAARASRRKTAHF"
     gene            1409..3070
                     /locus_tag="VH419_08210"
     CDS             1409..3070
                     /locus_tag="VH419_08210"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY7043635.1"
                     /translation="METNVKTNQYDAIVVGGGHNGLVAAAYLGRAGLRTLVLERRSST
                     GGAATTESPWPDQPDLRVTRLSYVMSLMPPSIIRDLELERHGYRVHPMGPYFQAFPEG
                     GSLTIHENDPARTHEEIAKFSRRDADAWHRWHEWLEGLAKHLGPLLLTVPPTIGSHAP
                     RELAALAKLAWRNRGADARVVADVTRLMTMSIADLLDDWFESAEIKGALAVNGVIGTW
                     AGPYEPGTAYVMAHHSIGDVGDGQLGSWGYPEGGMGAVSAAIRRAAEEHGVEIRTATP
                     VTRVLVANGRATAVVLADGEELRADTIVTTLHPRIAFLEHVGAEQLPAEFVEDIRHWK
                     SRSGVVKINLAMAELPNFTAKPNDTEVPEHLTGSVEMAPTMDYIERAFLDAREGRPAA
                     LPFSDSVIPTTLDRTLNPDGTHNFSMFTQWVPAEWSTEPHTEELDAYADRLIELYDAV
                     APNFKSAVIARDVVGPYEMEQEYGLVGGNIFHGELSLEQLFHMRPAPGYADYRTPIEG
                     LYYASSATHAGGGVCGIPGMQAAAAAVRDSRARRRDIRRWLASRG"
     gene            3063..5177
                     /locus_tag="VH419_08215"
     CDS             3063..5177
                     /locus_tag="VH419_08215"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HEY7043636.1"
                     /translation="MVDRRDPAERVGEAHAERMRRLLEPRSIAVVGASDRPGSFGDRL
                     TREVLRSPAAPTVSLVNPKYTTVHGRPCVPSLLDLDDAVDLVILGVPDSVLTDQLAQA
                     AERGDASALAFGTAHGLGDELASIAHRAGMAMCGGGSMGYVNNARGIRAIGYVERFPL
                     EPGGVAFITHSGSVFSSTLRTHRRLDFSLVVSAGQELVTSMADYLDYALDLDETRVVG
                     LFVETMRDADALQAAFSRAAAQDVPVVALTVGGSPTGRALVAAHSGAIAGDDAAWEAL
                     FAAHGVHRVHSLDEMADTLELFGIGRRVLPSSTGRIATVHDSGGQRALVADRADVHGV
                     PFAALSPTTTERLGRLLDPGLQPTNPLDVWGRGVDTEALVGTSLELLADDDAVAVVVA
                     ALDLAEEYDGDESYPRAVEALLHRTAKPVVVLSNIASAVDQRQAARLRAVGVPVLEGT
                     DSGLRALRHLLDQARPPETRPEATVDEGRARRWRTRLAAGDLDASAGLELLRDYGIAT
                     PRTVSVDSRAAAVAAAHECGFPVVLKTDAEAVLHKTDVDGVRTGLADADAVAARYEDM
                     SRRLGARAVVQAQVPSGVELALGVVRDPLLGPIVVVGVGGTLVEVLRQRQVALPPLSS
                     SDAERLLDRMPRLLELLAGVRGGAAADRAALTSAIVALGQLAVELGDEIEALDVNPLI
                     CRADGVFAVDALVVRRLPAGVS"
     gene            complement(5170..6390)
                     /locus_tag="VH419_08220"
     CDS             complement(5170..6390)
                     /locus_tag="VH419_08220"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY7043637.1"
                     /translation="MTSHPTVPTTADVVVVGGGTIGAWCAWFLRREGLDVVLVDAATL
                     GKGASSRAAGMVRAQGGTETAVRLGLYTREFYRDQRELLPFDSGFVEQGYFMPCFTEH
                     DVTEARARIAMQQRLGLDVRWVDPDEVDAMNPVIQPGMTLGASYAPGDGYIDPPMNVL
                     AYTAALVSSGVQVAEHTTFTGLETRGGRLVGVRTSRGDIATAMVVLTGGPTLSGIGRA
                     VGVRIPAGGARHQVVVTEDHPDLAAERLPMVFDVASGIYWRPEEGGILWGMSNPEESP
                     GIAHTFDWDYYDVARKRMAELVPLTAQLGLRKTWAATIDYTPDHLPILGPLITESGPV
                     AGTTVAAAGGHGMMWGPAVSRAAADLALHGTTDVVDTSDLGLDRFDEQGRSRLAADPI
                     ALPFPETTGDLSVS"
     gene            6458..7624
                     /locus_tag="VH419_08225"
     CDS             6458..7624
                     /locus_tag="VH419_08225"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY7043638.1"
                     /translation="MYGLSDADLALQETARAFAEELMPYEVEAEMNRGELENGMVDKH
                     RARAISLGLYATNMPVSVGGQGCSRLQQVLVQEQAGRVTNALGWVMATPPAWWVDVAS
                     EYQLERWVRPTVRGEMSECYAITEADAGSDVAALRATARREGDDYVLNGEKWHVTSFN
                     EASYVFFQAVLAEGDHAGEHGLFVVDLPSPGVRVVRTPEYAHTFAHHHPVVAFDDVRV
                     PAGHLVGAEGEGMTFSYDWFRFERLMVAARCLGAADRLVEEATAFAKDRIVGADRLID
                     RQLVQAMLADSLTELFAARSMVYETARSIDDGIDTKVAHARCSMVKLYASEMAGRVAD
                     RAVQIFGGRGYMRENVAERFTRELRVERIWEGASEIQRVIIADQLAKRGARALI"
BASE COUNT         1199 a         2637 c         2741 g         1165 t
ORIGIN      
        1 ggactccccg ctgatcgagc tggtcgagat cgaggggtcc cggcatcccg ggtctcgaca
       61 ggctcgaccg ccggactccc cgctgatcga gcttgtcgag gtcaaggggt cccggcagcc
      121 cgggtctcga caggctcgac cgccagactc ccgctgatcg agcttgtcga ggtcaaggag
      181 tcccggctgt ccggggtctc gacaggctcg accgccggac tccccgctga tcgagctggt
      241 cgagatcgag gggtcccggc atcccgggtc tcgacaggct cgaccgccgg acgttcgggc
      301 cgcgcctgct accttcctcc ggaaaagcca cctacgccac caaacggaca ggtgtcggtg
      361 gtggtttgcc tcggtagcca tccaaacgga cgggtagcca ggcagatctg ccttgggagg
      421 gatggatttg gctggttagc catgcaaacc gccgctcggc ggcaggggaa tgtccgaaaa
      481 accgccagtc acacgccggc caacaagggg acggaaaccc gggcggccag cccgtcaacg
      541 gcgaccagct ccgctcacca aacctcagaa gtgggcggtc ttgcggcgcg acgcccgcgc
      601 ggccgtcttc ttggcggggc gccgtttcgg tggcagtgcc agcgcccgtt cggcgaagct
      661 catcgcgatc tcgaacgccc ggttcgggtc gacgatcggg tcgttcaagg caacctggat
      721 cgagagtccg tcgagcagta ccgcccaggt gaccgcgaag tcgtcggcgt cgacgggctc
      781 gatctcgccg gcctcgatgc cgtccgccac gacgcctgcg atcgtcgacc gccagcgtct
      841 ctcgagctcg accctgtcgg tggcgacgag cggatgccgg aacgcctgtg cccacaggtc
      901 gaaccagagg ccccaagccc cagacatctc gttctcaccc tcgggaacgc aggtcagccg
      961 aacgagcttc tcgagccgat cacgcaacgc aggcgtgctt gccagcatct cggcacaggc
     1021 ggtgtagaag cggtcctcgg agtggcgcag cgctgcggtg agcaggtcgt ccttcgtccc
     1081 gaagtagtag atgacgaggg ccggcgacgt gccgatgcgg cgggcgacgt cggcgatccg
     1141 cgtctcggag aagccgcgct cgacgatgag ctccgcagcg gccgccagca tctgctcgcg
     1201 ccgctggtcg acgatgtcca cggcctcggt cttgccgaga ccatcgatcc cgtcgccctc
     1261 ggcctgtacc gcaagctcgg tgcgcctgcc tgccattgca cacccctcgc ttccccgtgg
     1321 gccttgtgga ggacgctatc acgttcataa actgaacagt cagtcagcat cccaggatgt
     1381 cgtgtggcgt acgagaggcg gcccggcggt ggagaccaac gtgaagacca accagtacga
     1441 cgcgatcgtc gtcggcggcg gacacaacgg gctcgtggcg gcggcctacc tcggccgagc
     1501 cggcctgcgg accctggtgc tggagcgccg ctcctcgacc ggcggcgccg cgacgaccga
     1561 gtcaccgtgg ccggaccagc ccgacctgcg cgtcacgcgg ctctcgtacg tgatgagcct
     1621 gatgccaccc agcatcatcc gcgacctcga gctcgagcgc catggctacc gcgtccaccc
     1681 gatgggtccg tacttccagg ccttccccga aggcggatca ctgaccattc acgagaacga
     1741 tccggcacgc acgcatgagg agatcgcaaa gttctcgcgc cgcgacgccg acgcgtggca
     1801 ccgctggcac gagtggctcg agggcctggc gaaacacctc ggcccgctgc tgctgaccgt
     1861 accgccgacg atcggttcgc acgcaccccg tgagctcgcc gcgttggcca agctcgcgtg
     1921 gcgcaaccgc ggcgccgatg cccgtgtcgt ggccgacgtg acgcggctga tgacgatgag
     1981 catcgccgac ctgctcgacg actggttcga gtcggccgag atcaaggggg cgctggcggt
     2041 caacggcgtc atcgggacat gggcgggtcc gtacgaaccg ggtacggcgt acgtcatggc
     2101 acaccactcc atcggcgacg tcggtgacgg gcagctcggc agctggggat accccgaagg
     2161 cgggatgggt gcggtctccg ccgcgatccg gcgcgccgca gaagagcatg gcgtcgagat
     2221 caggaccgcg actccggtga cccgggtgct cgtggccaac gggcgagcca cggctgtcgt
     2281 gctggcggac ggcgaggagc tcagggcgga cacgatcgtg acgacgctgc atccccgcat
     2341 cgcgttcctc gagcacgtcg gcgcggaaca gctgcccgcc gagttcgtcg aagacatccg
     2401 gcactggaag tcgcggagcg gcgtcgtcaa gatcaacctc gcaatggccg agctgccgaa
     2461 cttcacggcg aagccgaacg acacggaggt gcccgaacac ctcaccgggt cggtcgagat
     2521 ggcgccgacg atggactaca tcgagcgcgc cttcctcgat gctcgtgaag gccggcccgc
     2581 cgcactgccg ttctccgact cggtgatccc cacgacgctc gaccggacgc tcaacccgga
     2641 cgggacgcac aacttctcga tgttcaccca gtgggtgccg gccgagtgga gcaccgaacc
     2701 gcacaccgag gagctcgacg cgtacgccga ccggctgatc gagctgtacg acgcggtggc
     2761 gcccaacttc aagtcggccg tcatcgcacg ggacgtggtc gggccgtacg agatggagca
     2821 ggagtacggc ctcgtcggtg gcaacatctt ccacggcgag ctgtcgctcg agcagctgtt
     2881 ccacatgcgc ccggcacccg gctacgccga ctaccgcacg ccgatcgagg ggctgtacta
     2941 cgccagctcg gcaacccatg ccggcggcgg cgtgtgcggc atccccggca tgcaggccgc
     3001 cgcagcagca gtccgcgaca gccgggcccg gcgccgtgac atacgccgtt ggctcgcgag
     3061 ccgtggttga ccgccgagat cctgctgagc gggtgggcga ggctcatgcc gagcgcatgc
     3121 gccggctcct cgagccacgg tccatcgccg tcgtcggggc cagcgaccgc ccgggatcgt
     3181 tcggtgatcg gctgacgcgg gaggtccttc gcagtcccgc cgcaccgacc gtcagtctgg
     3241 tcaacccgaa gtacacgacc gtgcacggac gcccgtgcgt cccgtcactg ctcgacctgg
     3301 acgatgccgt cgacctggtg atcctcggcg tgccagacag cgtcttgacg gaccagctcg
     3361 cgcaggccgc cgaacgcggc gacgcgtcgg cactggcgtt cggcacggcg cacgggctcg
     3421 gcgacgagct ggcctcgatc gcacaccggg cagggatggc gatgtgcggg ggcgggtcga
     3481 tgggctacgt caacaacgcc cgcgggatcc gggcgatcgg gtatgtcgag aggttcccac
     3541 tcgagcctgg cggagtcgcg ttcatcaccc attccggctc tgtcttctcc tcgaccctgc
     3601 ggacccatcg gcgactggac ttctcactcg tcgtgtcggc cgggcaggag ctcgtgacgt
     3661 cgatggcgga ctacctcgac tatgcactgg acctcgacga gacccgcgtg gtcggcctgt
     3721 tcgtggagac catgcgtgac gccgacgcgc tgcaggcggc cttttcacgc gcggcggcgc
     3781 aggatgtccc ggtggtggct ctgaccgtgg gtggttcgcc gaccggccgg gctctggtgg
     3841 cagcccattc gggtgcgatc gccggtgacg acgcggcatg ggaggcgctg ttcgcggcgc
     3901 acggcgtcca ccgggtccac agtctggacg agatggccga cacgctcgag ctgttcggga
     3961 tcggccgccg tgtgctgccg agctcgaccg ggcgcatcgc gaccgtgcac gactccggcg
     4021 ggcagcgggc cctggtcgcg gaccgggcag acgttcacgg cgtaccgttc gccgcgctgt
     4081 cgcccacgac cacggaacgt ctcggccgac tgctcgaccc cggcctccag ccgaccaacc
     4141 cgctcgacgt gtggggtcgg ggagtggaca ccgaggcact ggtcggtacg tcgctcgagc
     4201 tgctcgccga cgacgacgca gtcgcggtcg tcgtcgcggc gctggacctc gcagaggagt
     4261 acgacggcga cgagtcctac ccacgggccg tcgaagcgct gctgcaccgg acggcgaagc
     4321 cggtcgtcgt cttgagcaac atcgccagcg cggtggacca gcggcaggcc gcccggctcc
     4381 gcgcggtcgg cgttccggtt ctcgagggca ccgactcggg cctgcgcgcg ctgcgccacc
     4441 tgctcgacca ggctcgacca ccggagacgc ggccggaggc gactgtggac gaaggtcgtg
     4501 cgcgtcgctg gcggacacgc ctggccgcgg gcgacctcga cgcgagcgcc ggcctcgagc
     4561 tgctccgtga ctacgggatc gcgacaccgc gcaccgtctc ggtcgacagt cgcgcggcag
     4621 cggttgccgc cgcccacgag tgcggctttc cggtggtgct caagaccgat gcagaagccg
     4681 tgttgcacaa gacggacgtg gacggcgtac gcaccgggct cgccgatgcc gacgccgtgg
     4741 cggcccgcta cgaggacatg agccggcgac tgggtgcacg tgcggtggtg caggcgcagg
     4801 tcccctcggg ggtcgagctg gcgttgggag tcgtgcgaga cccgctgttg ggaccgatcg
     4861 tcgtcgtcgg cgtcggcggc accctcgtcg aggtgctccg tcaacgccag gtggcgctgc
     4921 cgccgctgtc gagctcggac gccgaacggt tgctcgaccg gatgccgcgc ctgctcgagt
     4981 tgctggccgg cgtgcggggt ggtgccgccg ccgaccgggc agcgctgacg tctgcgatcg
     5041 tcgccctcgg acagcttgcg gtcgagctcg gcgacgagat cgaggcgctg gacgtgaacc
     5101 cgctcatctg ccgtgcggac ggcgtgttcg ccgtggacgc cctcgtcgta cgccggctgc
     5161 cggccggggt cagctgacgg acaggtcccc cgtcgtctcc ggaaacggca gcgcgatcgg
     5221 gtcggccgcc agccgactgc gaccctgctc gtcgaagcgg tccagtccga ggtcggaggt
     5281 gtcgacgaca tcggtcgtgc cgtggagggc caggtcggcg gcagcgcggc tcacggcagg
     5341 gccccacatc atcccgtgtc caccggcggc ggcgacggtg gtgccggcaa ccggaccgct
     5401 ctcggtgatg agcggtccga ggatgggcag gtggtcgggt gtgtagtcga tggtggccgc
     5461 ccacgtcttg cgcagcccga gctgcgccgt caacgggacg agctccgcca tccgcttgcg
     5521 tgcgacgtcg tagtagtccc agtcgaacgt gtgggcgatc cccggcgact cctccgggtt
     5581 gctcatgccc cagagaatgc ctccctcttc cggccgccag tagatgccgg acgccacgtc
     5641 gaagaccatg ggcagccgct cggcggcgag gtccggatga tcctcggtga cgaccacctg
     5701 gtgacgggcg ccgccggccg ggatccggac gccaacggcc cgcccgatgc ccgacaacgt
     5761 cggccctccg gtcaggacca ccatcgcggt ggcgatgtcg ccgcgcgatg tccgcacacc
     5821 gacgaggcga cctccgcgcg tctccagacc ggtgaacgtc gtgtgctcgg ccacctggac
     5881 ccctgacgag acgagcgcgg cggtgtaggc gagcacgttc atcggcgggt cgatgtaccc
     5941 gtcgccgggc gcgtaggacg cgcccagcgt cataccgggt tggatcacgg ggttcatcgc
     6001 gtcgacctcg tcggggtcga cccagcggac gtcgagaccg agcctttgct gcatggcgat
     6061 ccgcgccctc gcctcggtca cgtcgtgctc ggtgaagcac ggcatgaagt acccctgctc
     6121 gacgaagccg gagtcgaacg gcagcagctc gcgctggtca cggtagaact cgcgtgtgta
     6181 gaggccgagt cgaacggcgg tttcggtccc gccctgtgca cgtaccatgc cggcggcccg
     6241 cgagctggcg cccttgccga gcgtggccgc gtcgacgagt acgacgtcga gaccctcccg
     6301 gcggaggaac caggcgcacc aggccccgat cgtcccgcca ccgacgacga ccacgtcggc
     6361 ggtcgtcgga acggtcgggt gactggtcac aacatctcac gctgacatag actgaacagt
     6421 cagtcaagat cccccgagcg cgtgatatcg aggtgccatg tacggcttgt cagacgccga
     6481 tctcgccctg caggagacgg cccgggcatt cgccgaagag ctgatgccgt acgaggtcga
     6541 ggcggagatg aaccgcggag agctcgagaa cggcatggtc gacaagcacc gggccagggc
     6601 gatcagcctg ggtctgtacg cgacgaacat gccggtctcc gtaggtgggc agggttgctc
     6661 gaggctgcag caggtcctcg tccaggagca ggcagggcgc gtcaccaacg cgctgggctg
     6721 ggtgatggcg acacccccgg cctggtgggt cgacgtggcg agcgagtacc agctcgagcg
     6781 ctgggtgcgc cctaccgtgc gtggcgagat gtcggagtgc tacgcgatca ccgaggcgga
     6841 cgccgggtcc gacgtcgccg cgcttcgagc cacggctcgc agggagggcg acgactacgt
     6901 gctcaacggc gagaagtggc acgtcacgtc gttcaacgag gcgagctacg tcttcttcca
     6961 ggcggtgttg gcggagggtg accatgccgg cgagcacggc ctgttcgtcg tcgacctgcc
     7021 gagtcccggc gtacgcgtgg tgcgaacgcc ggagtacgcc cacacgttcg cgcaccatca
     7081 tcccgtcgtc gcgttcgacg acgtccgggt gccggccggg cacctggtcg gggccgaagg
     7141 tgagggcatg acgttctcgt acgactggtt caggttcgaa cggctgatgg tggcggctcg
     7201 ctgtctgggc gcggccgacc ggctggtcga ggaggcgacg gcgttcgcga aggaccggat
     7261 cgtcggagct gatcggctca tcgaccgtca gctggtgcag gccatgctcg ccgacagcct
     7321 gaccgagctg ttcgcagcgc gctcgatggt ctacgagacc gcgcgctcga tcgacgatgg
     7381 gatcgacacg aaggtcgccc atgcccggtg ctcgatggtc aagctgtacg cgtcggagat
     7441 ggccgggcgg gtcgccgacc gagcggtgca gatcttcggc ggccgtgggt acatgcgcga
     7501 gaacgtcgcc gagcgcttca cccgcgagct gcgcgtcgag cgcatctggg agggggcgag
     7561 cgagatccag cgcgtcatca tcgccgacca gctcgccaag cgcggcgcgc gcgccctcat
     7621 ctagtgccgc gtcaaccaac gttgctcgtc gtgagcggtg tccgggtgga tgagctgcaa
     7681 ggcagaggag ggaggcgttg cgcagcatcg ttgaccgacg ataacgccgc agatcgccgc
     7741 cc
//
LOCUS       DATBFZ010000266         6146 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_4685335, whole genome shotgun sequence.
ACCESSION   DATBFZ010000266 DATBFZ010000000
VERSION     DATBFZ010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 6146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6146
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4685335"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="VH419_08230"
     CDS             <1..467
                     /locus_tag="VH419_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012923117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY7043639.1"
                     /translation="SGKDQWEDDAHGLQLEFTDLAGNLQTAARELGATADPGLPCFAQ
                     LFLDAADVAAVRAFWVAALGYMHDRRPGLSDIHDPRRLNPVLAFQELDASETERRRQR
                     NRIHFELAVPSDLAQTRLARTLAAGGRLLDESEDRWRVADPEGNEMVIVSGA"
     gene            complement(653..2155)
                     /locus_tag="VH419_08235"
     CDS             complement(653..2155)
                     /locus_tag="VH419_08235"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HEY7043640.1"
                     /translation="MVTQSVDWVAIGPPLALVVAAIVALLADSFLSYRPTAGVVSLVG
                     IAVALWMTVATRDEARSTFCFAGGGAPTSCSWVVDDVTVAWWLIMLVATGLVVLLLWP
                     AVASGELPAGELHFLLLASASGALAVAAAADLITLLVAMETVTLPTFALVGLRRADRR
                     GSEAALKFFIASVIATAISLLGISLVYGATGSVVAAPVSASITAGGAITPVVGVGMIL
                     TVVALGFKVASVPFQVWVPDTYVGAPIPVAGYLSVVSKAAGLAGLVIVLVRFMTSYVD
                     SWSAVVAIAAALTMTVGNLGALRQRHVVRLLAWSSVAQAGYLLVPLAAGGTSDDVRAM
                     LAYALMYAVVNLAAFAAAVVVGSWGKSDVDDCAGLARTHPWIGGALAFALLCLAGLPP
                     GVVGLIAKVAIFQSAVDGAGVDTTWLAVVMAVNVAIGLVYYLRFLVVLFRRAPDPAPA
                     PGSAESVEEGDGWAGLDRAVPWSTEFVVGSTLAASVLFSIFPGLLFSAIS"
     gene            complement(2163..3644)
                     /locus_tag="VH419_08240"
     CDS             complement(2163..3644)
                     /locus_tag="VH419_08240"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020578354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEY7043641.1"
                     /translation="MGGIEVLIGLPLAGSVVVLLLGRRPIAATWVGVLVTSATLVGSV
                     VPWVATIDDDGRYVARSDADWIRPLDVHWSLGLDALSAPLVVLTALLSWCCLVALVRR
                     APAYGSRPGLVALVLVIEGASIGTFAAVDVIVFFVFFELVLIPMWFVIADWGDEHDPI
                     GRRRAATRFLVVTVLGSALVLIAFLVLHASTGTFDVVELQARSGLVGHGTQVLVAVLL
                     VLGFGAKVPMWPLHFWLPDAHSKAPTVGSVLLAGVLLKLGTYGLLRFWFDLVPSGAVS
                     VAPYLGGLGVVGIIYGSLACLAQTDLKRLVAYSSVGHMGFALLATSTLTSQGRLAANF
                     VNVAHGVITGLLFFVVGGVKERVGETSLESIGRAWYGRAPHLAAVFTFGAVASLGLPG
                     LAGFWGEMLSMLAAFDPAASLDRSTYLVYMVVAGIGAILTTAYFVVAIRRVCQGVIDR
                     DAVPDVAEDEWIAWSPLVGSVVALGLFPVIMLWSASSSLVAGG"
     gene            complement(3644..5485)
                     /locus_tag="VH419_08245"
     CDS             complement(3644..5485)
                     /locus_tag="VH419_08245"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020578355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEY7043642.1"
                     /translation="MTVVVLVALVAAAVGSLVVGHRDDRIAVILGVAGGAVAFVASVV
                     VGVQHWGTGAAESVTGSGIVETGGLPISITLRVDQLSASVLVLATTVALLVQVYSVAY
                     LRGDPRFPTYSAFVSIFTAAMALVVTADDLFVLLVGWEVMGVCSYILIGHHWELEPAR
                     SGAVKAFLMTRLGDVGMLFGFFVLGNAAGTYRISGILDQALAGGISENQATLSTLLIL
                     CGVVGKSAQFPLHSWLPDAMPGPTPISALIHAATMVAAGVFLVARLLPVFELSTVSMT
                     VLLVIACVTMLGSALFALAADDLKRLLAWSTVSQLAYMFAALSLGGYSAGVLHLISHG
                     AFKALLFLAAGSVIHAVGTQRLDGMGGLRVSMPVTFVTMTIGFAALAGVPPLFGFFSK
                     DGVLGVASDEALHGSAARGWLVLLVGFLVAGLTASYSTRAWLLTFFGPRRGDTTATPP
                     HESPWLMLGPMVALAAATVVGGVLVLQPTFLGVDAEEWHPVVMVVSLAVVAGGVLLTL
                     GEWARVQKTDPAVSLGRLRPVLAREMEYDGLVDKGVTRPTWWLARMTRLNEDEVVEAY
                     VGGTDSGSQWAARLIRWSHNGNAQVYATSIVIGAVAVAIVIGVVGAS"
     gene            complement(5482..5832)
                     /gene="nuoK"
                     /locus_tag="VH419_08250"
     CDS             complement(5482..5832)
                     /gene="nuoK"
                     /locus_tag="VH419_08250"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020578356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEY7043643.1"
                     /translation="MPLLFPLVLSSVLFGTGVYVVLARRHAVLVLMGVELMLNAVNLN
                     LVAFDAWWHDRLDSGQVLALYVIAIAAAEIGLGLAIVLLVFRTHRHVDVDRLRELADR
                     DPATVSDLVGGSAS"
     gene            complement(5832..>6146)
                     /locus_tag="VH419_08255"
     CDS             complement(5832..>6146)
                     /locus_tag="VH419_08255"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEY7043644.1"
                     /translation="VLLLFALMLTRAPIGAVPDLTGTNRFIAAIAGLATTGMLVVTFV
                     AGFEDTRIDVGAGDIGSTRALGGTLFGSWVLPFEIVSVILLAALVGAIVLSRRREPGD
                     AS"
BASE COUNT         1212 a         2194 c         2058 g          682 t
ORIGIN      
        1 acagcggtaa ggaccaatgg gaggacgacg cccacggcct ccagctcgag ttcaccgacc
       61 tcgccgggaa cctccagacc gctgcccgcg aactcggggc caccgcggat cccgggctgc
      121 cgtgtttcgc tcagctcttc ctcgacgccg ccgacgtcgc cgcggtccga gcgttctggg
      181 tcgctgcact cggatacatg cacgaccggc gacccgggct cagcgacatc cacgatccgc
      241 ggcggctgaa cccggtgctg gcgttccaag agctcgatgc ttcggagacg gagcggcgcc
      301 gacagcgcaa ccgcatccac ttcgagctcg ctgtgccgtc ggacctcgcg cagacgcgcc
      361 tcgccaggac tctcgcagcc ggtggccggc tgcttgacga gtcggaggat cgctggcggg
      421 tcgccgaccc cgaaggcaac gagatggtga tcgtcagtgg cgcatgagct ggcgttcggc
      481 tgagccccac gctcggccgt ttcccgtcaa cgagcgctgg atcttcgccg cagccggcat
      541 cgaacacgct cgccacgcct gtatgacggc ccggcccggg tcgcgcaggt cgctggcgac
      601 ctcgggcgcc gcgcggcacg tcgcgccact gagacggacg ggtagcccgc agtcaggaga
      661 tggcggagaa caacaacccg gggaagatcg agaacagcac tgacgccgcg agtgtgctgc
      721 cgacgacgaa ctcggtggac cagggaacgg cgcgatccag gcccgcccag ccgtcaccct
      781 cctcgaccga ctcggccgag cccggggcag gcgccgggtc gggggctcgt ctgaacagca
      841 cgaccaggaa ccgcaggtag tagacgaggc cgatggcgac gttgacggcc atgacgaccg
      901 caagccaggt cgtgtcgacc ccggcgccgt ccaccgcact ctggaagatg gcgaccttgg
      961 cgatcaaccc gaccacgccc ggtgggagcc cggccaggca gagcagcgcg aacgccagcg
     1021 caccgccgat ccatgggtgg gtgcgcgcca ggcccgcgca gtcgtcgacg tcggacttgc
     1081 cccagctgcc cacgacgacg gcggcggcga aagcagcgag gttcacaacg gcgtacatca
     1141 acgcgtacgc cagcatcgcg cgtacgtcgt cactcgtccc acccgcggcg agcggaacga
     1201 gcaggtagcc cgcctgcgcg acagacgacc aggccaacaa ccgcacgaca tggcgttggc
     1261 ggagcgctcc gaggttcccg acggtcatcg tcaaagccgc ggcgatggcg acgacggccg
     1321 accacgagtc gacgtacgac gtcatgaagc ggacgagcac gatgaccaac ccagccaggc
     1381 cggcggcctt gctcacgacg gagaggtaac ccgccaccgg gatcggcgcg ccgacatagg
     1441 tgtccggcac ccacacctgg aacggcaccg acgcaacctt gaagcccagc gccaccacgg
     1501 tgaggatcat gccgacgccg accaccgggg tgatcgcgcc gccggcggtg atggaggcgg
     1561 acaccggtgc ggcgaccacc gagccggtcg cgccgtacac cagcgagatg ccgagcaggc
     1621 tgatcgcggt cgcgatgacc gaggcgatga agaacttcag cgccgcctcc gagccacgac
     1681 gatcggcccg gcgcagcccg accagcgcga acgtcggcaa cgtgacggtc tccatcgcga
     1741 ccagcagcgt gatcaggtca gcagcggcgg cgacggcgag agctccggag gcagaggcca
     1801 agagcaggaa gtgcagttcg ccagcgggca gctcgccgga agcgacggcc ggccacagca
     1861 gcagtacgac gagtccggtc gcgacgagca tgatcagcca ccaggcgacg gtgacgtcgt
     1921 cgacgaccca cgagcagctc gtcggcgcac cgccgccggc gaagcagaac gtcgagcgtg
     1981 cctcatcccg ggtggcgacc gtcatccaca acgccaccgc gatccccacg aggctcacga
     2041 cacccgccgt cggacggtac gacaggaacg agtcggccag cagcgccacg attgcagcga
     2101 ccacgagcgc gagcggcggc ccgatcgcca cccagtcgac ggactgcgtc accattgggg
     2161 catcacccgc ccgcgaccag gctcgacgac gccgaccaga gcatgatcac cgggaacagg
     2221 ccgagtgcca ccaccgaacc gaccagcggg gaccacgcga tccattcgtc ctcggcgacg
     2281 tcgggtacgg cgtctcggtc gatcacgcct tgacagacac ggcgaatcgc gacgacgaag
     2341 tacgcggtcg tgaggatcgc gccgatgcct gccacgacca tgtagaccag gtacgtcgac
     2401 cggtccagcg acgcggccgg gtcgaacgcc gccagcatgg acagcatctc cccccagaag
     2461 ccggcgagac cgggaaggcc gaggctcgcc actgcgccga acgtgaagac ggcagccagg
     2521 tgcggagcgc ggccgtacca ggcgcgaccg atcgactcga ggctcgtctc acccacgcgc
     2581 tctttcacgc cgccgaccac gaagaacagc agcccggtga tgaccccgtg cgcgacgttg
     2641 acgaagttcg ccgccaacct gccctgactc gtcagcgtcg acgtcgcgag caacgcgaag
     2701 cccatgtggc ccacgctgga gtaggcgacc agccgcttga ggtcggtctg agcgagacag
     2761 gcgagggagc cgtagatgat cccgacgacg ccgagtccac cgaggtacgg cgcaaccgac
     2821 accgcgccag acggcacgag gtcgaaccag aagcgcagca gaccgtacgt gccgagcttc
     2881 agcagcacgc ccgcaagcag cacagagccg accgtgggcg ccttgctgtg cgcgtcgggc
     2941 aaccagaagt gcaacggcca catcggcacc ttcgctccga acccgagcac cagcagcacc
     3001 gcgacgagca cctgcgtccc atgacccacc agcccggacc gggcctgcag ctcgaccacg
     3061 tcgaaggtcc cggtggaggc gtgcagcacc aggaaggcga tgagcaccag ggcagagccg
     3121 agcacggtga cgaccaggaa tcgggtggcg gccctccggc ggccgatcgg atcgtgctcg
     3181 tcgccccagt ccgcgatgac gaaccacatc gggatcagca ccagctcgaa gaacacgaag
     3241 aacacgatca cgtcgaccgc ggcgaatgtt ccgatgctgg caccttcgat gacgaggacg
     3301 agtgccacga ggccgggtcg agacccgtac gccggtgcgc gacgcaccaa cgcgacgaga
     3361 cagcaccacg acagcagggc ggtgagcacg accaacggtg ccgacaaggc atccagcccg
     3421 agcgaccagt gcacgtccag cggcctgatc cagtcggcgt cgctgcgcgc gacgtacctg
     3481 ccgtcgtcgt cgatggtcgc gacccacggg acgactgagc cgacgagggt cgcggacgtc
     3541 accagcacgc cgacccaggt cgccgcgatc ggacgtcgtc cgagcaacag caccacgacc
     3601 gagccggcaa gcggaagccc gatcaggact tcgatgccgc ccatcaggag gcacccacca
     3661 cgccgatgac gatcgcgacc gccaccgccc cgatgacgat cgacgtcgcg tacacctggg
     3721 cgttgccgtt gtggctccag cggatcagcc gggcggccca ttgactcccc gagtcggtcc
     3781 cgccgacata tgcctccacg acctcgtcct cgttgagccg agtcatccgc gcgagccacc
     3841 aggtcgggcg ggtgacaccc ttgtcgacca gcccgtcgta ctccatctca cgcgccagca
     3901 cgggccgcag ccgcccgagc gagacagccg ggtcggtctt ctgcacgcgc gcccattcgc
     3961 cgagggtgag cagcacgccg cccgcgacga ccgcgagact caccaccatc acgaccggat
     4021 gccactcctc cgcatcgacc ccgaggaacg tcggctgcag caccagcacg ccgccgacca
     4081 cggtcgccgc ggccagcgca accatcggac cgagcatcag ccacggcgac tcgtgcggcg
     4141 gtgtcgccgt ggtgtctcca cgtcgtgggc cgaagaacgt cagcagccag gccctggtgg
     4201 agtacgacgc ggtgagtccg gccaccagga agccgacgag gaggaccagc cagccccgag
     4261 cggccgaccc gtgcagcgcc tcgtcactcg ccacgccgag cacgccgtcc ttggagaaga
     4321 acccgaacag cggaggcaca ccggccagcg ccgcgaaacc gatcgtcatc gtcacgaacg
     4381 tcaccggcat cgacacccga agcccgccca tgccgtcgag ccgttgcgtg ccgacggcgt
     4441 ggatgacaga gcctgccgcg aggaacagca gcgccttgaa cgcgccatgg gagatgaggt
     4501 gcagcacgcc ggcggagtag ccgcccaggg acagcgcggc gaacatgtag gccagctggc
     4561 tcaccgtcga ccacgccaac agccgcttga ggtcgtccgc ggccaacgcg aacagggccg
     4621 agccgagcat ggtgacgcac gcgatcacga gcagcacggt catcgacacg gttgacagct
     4681 cgaacacggg tagcagccgc gccaccagga acacaccggc tgcgaccatc gtggcggcgt
     4741 ggatcagggc gctgatcggc gtcggtccgg gcatggcgtc gggcagccac gagtgcagcg
     4801 ggaactgtgc cgacttgccg acgacgccgc agaggatcaa caacgtcgac agcgtcgcct
     4861 ggttctcgga gatgccgcct gccagcgcct ggtcgaggat gccgctgatg cggtacgtcc
     4921 cggcggcgtt gccgagcacg aagaagccga agagcatccc gacgtcgccg aggcgggtca
     4981 tgaggaacgc cttgaccgca cctgatcgcg ccggctcgag ctcccagtgg tgtccgatca
     5041 ggatgtagga gcagacaccc atcacctccc acccgaccag cagcacgaac aggtcgtcgg
     5101 cggtgactac gagcgccatg gccgcggtga agatgctgac gaacgcggag tacgtcggga
     5161 agcgcgggtc accacgcagg tacgccacgg agtacacctg aacgaggagc gcgaccgtgg
     5221 tggcgagcac caggaccgag gcgctgagct ggtcgacccg aagcgtgatg ctgatcggca
     5281 ggccgcccgt ctcgacgatg cccgatccgg tcaccgattc cgcggcgccc gtcccccagt
     5341 gctggacacc gacgacgacc gacgcgacga aggcgaccgc accgcctgcc acaccgagga
     5401 tcacggcgat gcgatcgtcc cggtggccga ccacgagcga acccactgct gcggcgacca
     5461 gcgccaccag cacgacgacc gtcatgaggc agacccgccg acaagatcgg acacagtggc
     5521 cgggtcacga tcggcgagct cccgcagcct gtcgacgtcg acgtggcggt gggtacggaa
     5581 gacgagcagc acgatcgcaa gtccgaggcc gatctcggcc gcggcgatcg cgatgacgta
     5641 gagcgcgagc acctgccccg agtcgagccg gtcgtgccac caggcgtcga aggcgaccag
     5701 gttgaggttg accgcgttga gcatcagctc cacgcccatc aggacgagca cggcatgccg
     5761 tcgggccagg acgacgtaca ccccggtgcc gaacagcacc gaggacaaga cgagcgggaa
     5821 cagcagcggc atcaggaggc gtctcccggc tcgcgccggc gggacagcac gatcgcgccc
     5881 accagcgcgg ccagcaagat gacggagacg atctcgaacg gcagcaccca ggagccgaag
     5941 agcgttccgc ccagggctcg cgtgctgccg atgtcaccgg caccgacgtc gatgcgggtg
     6001 tcctcgaacc cggcgacgaa ggtcaccacg agcatcccgg tggtcgcgag gcccgcgatc
     6061 gcggcgatga accggttcgt gcccgtcagg tcgggcacgg cgccgatcgg cgcgcgggtg
     6121 agcatcaggg cgaagagcag caggac
//
LOCUS       DATBFZ010000267         2503 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_5015701, whole genome shotgun sequence.
ACCESSION   DATBFZ010000267 DATBFZ010000000
VERSION     DATBFZ010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5015701"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(86..946)
                     /locus_tag="VH419_08260"
     CDS             complement(86..946)
                     /locus_tag="VH419_08260"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012920720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY7043645.1"
                     /translation="MDEDELDVADEFAGGVTRRIVTAPENLRASRTWWDADADAYQSA
                     HGAFLGEVELRWCPEGLREEDARLLGDVAGRRIVEVGCGAAAGARWLVTQGAHVVALD
                     VSAGMLRHARIAAAHSGVPVPLLQADATFLPFPDAGLDSGFDVAFTAFGAVPFVDDSA
                     AVMREVFRILRPGGLWVFAVTHPMRWIFLDDPGERGLVAVHSYFDRRPYVEEDESGTP
                     TYVEHHRTLGDRVRELVDAGFVLTDIVEPEWPDSNHEVWGQWSPLRGRVFPGTAIFCA
                     TKPAYRAPSK"
     gene            1622..>2503
                     /locus_tag="VH419_08265"
     CDS             1622..>2503
                     /locus_tag="VH419_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020388850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEY7043646.1"
                     /translation="MTSSIEATSSANQIAVDDLGNEEDFLAAVDATIKYFNDGDIVEG
                     TIVKVDRDEVLLDIGYKTEGVIPSRELSIKHDVDPSEVVNVGDHIEALVLQKEDKEGR
                     LILSKKRAQYERAWGTIEKIKEDDGVVRGSVIEVVKGGLIIDIGLRGFLPASLVEMRR
                     VRDLQPYVGRELEAKIIELDKNRNNVVLSRRAYLEQTQSEVRTEFLNKLAKGQVRKGV
                     VSSIVNFGAFVDLGGVDGLVHVSELSWKHIDHPSEVVEVGMEVEVEVLDVDLDRERVS
                     LSLKATQEDPWRQFARTH"
BASE COUNT          449 a          840 c          826 g          388 t
ORIGIN      
        1 cttcttgtct tgaaagccat gtgtctcaag cgattcaggc gtcgaatgat tcccgtgtga
       61 tgctgtgttc ccgatcacgc atgggtcatt tcgaaggtgc gcggtaggcc ggcttggtcg
      121 cgcagaagat tgcggtaccc gggaaaaccc ggccacgaag cgggctccac tgtccccaca
      181 cctcgtggtt gctgtccggc cactcgggtt ccacgatgtc ggtcagcacg aacccggcgt
      241 cgacgagttc gcggacgcgg tcgccgagcg tccggtgatg ctcaacgtac gtcggcgtac
      301 cggactcgtc ctcctcgaca tacggacggc ggtcgaaata cgagtgcacc gcgaccaggc
      361 cacgctcgcc gggatcgtcg aggaagatcc accgcatcgg gtgcgtcacc gcgaacaccc
      421 ataggccgcc cggtcgcagg atccggaaca cctcgcgcat cacggccgcg gagtcgtcca
      481 cgaacggaac cgcaccgaat gcggtgaagg ccacgtcgaa tccactgtcg agcccggcgt
      541 ccgggaacgg caggaacgtc gcgtcggctt ggagcagcgg taccggtacc cccgaatgcg
      601 cggcggcgat gcgcgcgtgc cgcaacatcc cggcggagac atcgagcgcg acgacatgcg
      661 cgccctgcgt caccagccac cgcgcgccgg cggccgcgcc gcatccgacc tcgacgatgc
      721 ggcgtccggc gacatcgccg agcagccgcg catcctcctc acgcaggccc tccgggcacc
      781 agcggagttc gacctcacca aggaacgcgc cgtgcgcgga ctggtacgcg tcggcgtccg
      841 catcccacca cgtgcggctg gcgcgcagat tctccggcgc cgtgacgatc cgccgcgtca
      901 cgccgccggc gaactcgtcc gcgacgtcca gctcgtcttc gtccatgcgg cataacgtac
      961 ggccacgccg ggactcccgg gccgaaagcg ccgtgcgaac gcttctcgtc gcaaaagtcg
     1021 gcggacggca tcggcgcgcc gcgacggtcc ggacgcgcaa cttcgatagc gtcgtcggca
     1081 gcggagttgt ccagtgtgga ccgccgaccg cacggccggc cgaccacggc ggatcgcgac
     1141 gtcatgcaac gaaccggatc cactcggagg agcaccggtt ggtgccgcgc cagcgggtcg
     1201 ggaggtccga gcagctagag ggcgtctccc accggatgtt cggcggctga cccgcccgcg
     1261 ccgcgcgacc ggctcggcta gtccggaccg ggaccgcgcg gactccgccg gcacggcgat
     1321 cgacggatcg ccgtgccggt ttgtccttta tcggtaggaa aaagcccatg tgtcgctgat
     1381 gccgaagagc agtgcatcgc gtactgggtg gttgcacctt gcggcattgc gcacggtaag
     1441 ctaatcgatg cgctcgtggg ctgcgcgcct cagtagggag caggtccgcg gccggcatca
     1501 cacctcaacc gatcatcgtt cgatcggatg agccgtgcct gtcggcaggc cggcgggcgc
     1561 agacccggtc tcccgccgac cggatcacca gcgaccatat ccgaccggag caacagccca
     1621 catgacgagc agcatcgagg ccacctcgag cgccaaccag atcgcggtcg acgacctcgg
     1681 caatgaggaa gactttcttg ccgcggttga cgcgaccatc aagtacttca acgatggcga
     1741 cattgtcgaa ggcaccatcg ttaaggtcga cagggacgag gtcctactcg acatcggcta
     1801 caagaccgag ggtgtcatcc cctctcgtga actgtcgatc aaacacgacg tcgacccgtc
     1861 tgaggtcgtc aacgtcggtg accacatcga ggccctggtt ctccagaagg aggacaagga
     1921 gggccggctg atcctgtcca agaagcgcgc gcagtacgag cgcgcctggg gcacgatcga
     1981 gaagatcaag gaagacgacg gtgtggtccg tggctcggtc atcgaggtcg tcaagggtgg
     2041 tctgatcatc gacatcgggc tgcgcggctt cctgcccgcg tcgctcgttg agatgcgccg
     2101 ggtacgggac ctgcagccgt acgtcggccg cgagctcgag gcgaagatca ttgagctgga
     2161 caagaaccgc aacaacgtgg tgctgtcgcg gcgcgcttat ctcgagcaga cccagtccga
     2221 ggtgcgcacc gagttcctca acaagctcgc caagggccag gtccgcaagg gcgtcgtgtc
     2281 gagcatcgtg aacttcggcg cgttcgtcga cctcggtggc gtcgacggcc tcgtgcacgt
     2341 ctccgagctg tcctggaagc acatcgacca cccgtccgag gtggtcgagg tcggcatgga
     2401 ggtcgaggtc gaggtgctcg acgtcgatct cgaccgggag cgcgtctcgc tgtcgctcaa
     2461 ggcgacgcag gaagacccgt ggcgccagtt cgcccgtacc cac
//
LOCUS       DATBFZ010000268         1698 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_178626, whole genome shotgun sequence.
ACCESSION   DATBFZ010000268 DATBFZ010000000
VERSION     DATBFZ010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178626"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..754
                     /locus_tag="VH419_08270"
     CDS             <1..754
                     /locus_tag="VH419_08270"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY7043647.1"
                     /translation="TGLTDVPIFAVDLSGSWALFGADVDRDTLVDLVDPEHDRFEWLA
                     PADCARRILPPWVADAQVARGAQVPMLDVRFRPMRRDDIGSVLAWQQAPHAERWFHGD
                     RMTLEAAQERYGARIDGDRPVHMWAVSFAGHDAGYLQHYRIGDLDDEYATKTRDPEAV
                     GFDYLIGDPTYIGRGLGTRMIWTFLRDVLLDACPEAPRFLASPDHRNAASLRALAKCG
                     FVAGDWIDMPSASGEGMTTEVVCTLDRRHWFG"
     gene            988..>1698
                     /locus_tag="VH419_08275"
     CDS             988..>1698
                     /locus_tag="VH419_08275"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEY7043648.1"
                     /translation="MKPLPAEYFVNYGTNAEMRWSSVYDQGYLTSNDRFFVRDHTRTP
                     IIDPAMYELKVYGDGLAAARTVDTAFTFSYRDLQHMPKRCVTSFIECTGNGRSFFATQ
                     QGAPATGTQWTLGAIGVATWRGVPLRHLLHRVGLRRDAVDVMATGLDDAYVSGGVDYG
                     RVRRPIPVRKALDDVLLVLEMNGEPLPPDSGFPARLLVPGWVGIASIKWLGELQVSRT
                     ALTSPWNTKWYRMTGGDYP"
BASE COUNT          266 a          568 c          593 g          271 t
ORIGIN      
        1 gaccgggctg accgacgtgc cgatcttcgc ggtcgacctc tcggggtcgt gggcgctgtt
       61 cggcgcggac gtggatcgcg acacccttgt ggacctggtc gatccggagc acgaccggtt
      121 cgagtggctg gctccggccg actgtgcgcg acggatcctt cctccctggg tggccgacgc
      181 gcaggtcgcc cgcggcgcgc aggtgccgat gctcgacgtg aggttccggc cgatgcgacg
      241 cgacgacatc gggtccgttc tggcctggca acaggcgccg catgccgagc gttggttcca
      301 cggagaccgg atgacgctcg aggcagcgca ggagcggtat ggagcgcgca tcgacggtga
      361 ccgtccggtg cacatgtggg ccgtctcgtt cgccggtcac gatgccggat acctccagca
      421 ctatcgcatc ggggacctcg acgacgagta cgcgacgaag acacgagatc ccgaggccgt
      481 cggcttcgac tacctcatcg gcgacccgac gtacatcggc agggggctcg gcacccggat
      541 gatctggacg ttcctgcgtg acgtgctgct cgacgcctgc ccggaggcgc cgcggttcct
      601 ggccagcccc gaccaccgca acgcggcctc gttgcgtgcc ctggcgaagt gtggcttcgt
      661 cgccggcgac tggatcgaca tgccgagcgc gtccggtgag ggcatgacga ccgaagtggt
      721 gtgcaccctg gaccgccgtc actggttcgg ctgaacacgg caccggatcc tgcgacacag
      781 agcactcctc ctctcgatgt ggctgtaagt agagtgaata actctagtat gaggccatga
      841 cctcatcaca catgccgcag gacgccgccg tcgccgactt ggagctcagc cggcgacggc
      901 tgctccagtc cgctgcagcc ggtgccgcgt tggccggcgc gggcgcgctc gtccagacgc
      961 cgcgcccggg ccaccgacgg cccgatcgtg aaaccgctcc cggcggagta cttcgtcaac
     1021 tacggcacga acgccgagat gcgctggtcg tcggtatacg accagggcta cctgaccagc
     1081 aatgaccggt tcttcgtgcg cgaccacacc cggacaccga tcatcgatcc ggcgatgtac
     1141 gagctcaagg tgtacggcga cgggctggca gcggcgcgga ccgtcgacac ggccttcacg
     1201 ttctcctacc gcgacctgca gcacatgccg aagcgctgcg tgacctcgtt catcgagtgc
     1261 acgggcaacg ggcggagctt cttcgcgacc cagcaggggg cgcccgcgac gggaacgcag
     1321 tggacactcg gcgcgatcgg cgtcgcgaca tggcgcggcg tgccgctgcg ccatctgctg
     1381 caccgcgtgg ggttgcgcag agacgccgtc gacgtgatgg cgaccggcct ggacgatgcg
     1441 tacgtgagcg gtggcgtcga ctacgggcgg gtgcgccggc cgatcccggt caggaaggcc
     1501 ctcgacgacg tgctgctcgt cctcgagatg aacggcgagc cgttgccgcc ggacagcggc
     1561 ttcccggccc gactgttggt gcctggctgg gtcggcatcg ccagcatcaa gtggttggga
     1621 gagcttcagg tctcgaggac ggcgctcacg tcgccgtgga acaccaagtg gtaccggatg
     1681 accggcggtg actacccg
//
LOCUS       DATBFZ010000269         3746 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_2151741, whole genome shotgun sequence.
ACCESSION   DATBFZ010000269 DATBFZ010000000
VERSION     DATBFZ010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 3746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3746
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2151741"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(168..362)
                     /locus_tag="VH419_08280"
     CDS             complement(168..362)
                     /locus_tag="VH419_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HEY7043649.1"
                     /translation="MRTNIDIDDDLLAEALNVSGAATKKEAVHRGLELLVRLGRQSEV
                     RQLRGRLRWDGDLEAQRLDR"
     gene            691..2151
                     /locus_tag="VH419_08285"
     CDS             691..2151
                     /locus_tag="VH419_08285"
                     /inference="COORDINATES: protein motif:HMM:NF023283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3375 domain-containing protein"
                     /protein_id="HEY7043650.1"
                     /translation="MGATMQYDEIDHLQAHHPAWVLLRSPHAPLILSFLARVFVEGNV
                     GGQPAQALIGLLDDELFALNARAEGDPPFPRPAQAYLDEWASPEKGWLRKFYPSGIDE
                     PHYDITPPVEKAVLWLKDLRRREFVGTESRLNTLFELLRQMVFGADTDPESQLADLRR
                     RRAELDEQIARAERGEVALLDATSQRDRYYQFARNARELLADFREVEENFRILDRRLR
                     EQIAGWSGSKGALLDEALNSRNSITESDQGRSFQALFDFLLSRQRQAELSELLDRLGQ
                     ISELDEIDPRLRHVHFDWIDAGERTQGTVRLLSEQLRRFLDDQVWLENRRIFDLLHSI
                     EATAVELRNRPIGLAMELPGMGVEVNLPFERPLYTPIAPVSLESDAVEVGSTDFDATA
                     LLDQLYVDRDELARTVRSSLSGRSQVGLAPLIADHPLHQGLAELIGYLSLSDPAFDVV
                     FDEDDRDRIGWTTDETERVADTPSVTFARRTTGGTS"
     gene            2148..2744
                     /locus_tag="VH419_08290"
     CDS             2148..2744
                     /locus_tag="VH419_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020388453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4194 domain-containing protein"
                     /protein_id="HEY7043651.1"
                     /translation="MSQATLEASELTAVVVQLMKGPVYRDTHEKIWHQLLAVRNQASD
                     YVAVLGLQVMIDEAEGYAFLRARPVEETGEDLPRLVPRRALSYPVSLLLALLRKKLAE
                     FDAVSSDSRLVISREQLVELIRTFLPDTPNEARLVATIDTHIGKVVDLGFLRPLRGQP
                     DQFEVRRILKAYVDGQWLSQLESRLRGYRDQLAGEEAR"
     gene            2741..>3746
                     /locus_tag="VH419_08295"
     CDS             2741..>3746
                     /locus_tag="VH419_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020388454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY7043652.1"
                     /translation="MTDALFSTLEVEDADPSRTPGTRLRRLEVYNWGTFHNRVWTFDV
                     AGRNALLTGDIGSGKSTLVDAVTTLLLPSHRISYNKAAGADTRERDLRSYVLGHYRSE
                     RNEITGASRPVALRDTRSYSVLLGVFGNADFDTTTTLAQVFWTRDGQSGQPDRFYVVA
                     DGDLSIAEHFAGFGTDVTQLRNRLQRMGLKVHKAFPEYGRELRRRLGIESEQALELFH
                     QTVSMKAVDNLNDFVRHHMLEPFDVGHQIEALVRHFEDLTRAHEAVVRAKAQLELLGP
                     LIADLDTHDGLAAEVADLGHQRAALPYFLARRRERLLAAELAETEQRRAALAAEETTT
                     R"
BASE COUNT          622 a         1294 c         1238 g          592 t
ORIGIN      
        1 atgcgcggca cggaaatcgg catcatgccg gaacccctgg agcacctcgg tcagaatcag
       61 atcgccgacg aggaggggtc gctgacccaa gagccggtcg accaggtcca cctcgggcga
      121 cgctgctccg ttgaagaagt cgatccagac gccgctgtcg acgacgatca ccggtcgagc
      181 cgctgcgcct cgagatcgcc gtcccagcgc agtcgccccc gcagctgtcg cacctcggac
      241 tgccgtccga gccggaccag cagttcgagt ccccggtgaa cggcttcctt cttggtcgcg
      301 gcgccgctga cattcagggc ctcggcgagc aggtcgtcgt cgatgtcgat gttggtacgc
      361 atgctgctcc tccacccatg tgtatgactc gattaaattg tacacatcgg gctgcccgga
      421 gtctcagacg aaagaggccg ccttgggcct ggacggcctg aatctggggg cctgacgctc
      481 gacggcttgg acttcggcgg tcccagcgat ctggacctcg cgaatctcga cctcgacgat
      541 atgtagctcg acggggaggg gtcgagaact ccagggcacc tatctcaccg gcacgctgcg
      601 gggctacatc gtggcccgac gagacgctct ctgaccgatt tgcgccctcg agccggtcgc
      661 aaccgggaac tgtcagtgcg ccgtgccagc atgggcgcca ccatgcagta cgacgagatc
      721 gaccacctgc aagcccacca tccggcctgg gtccttttgc gttccccgca cgctccgctg
      781 atcttgtcgt tcctggcccg ggtgttcgtc gagggcaacg tcggcggtca gccggcccag
      841 gccttgatcg gcctgctcga cgacgaactg ttcgcgctga acgcccgcgc cgagggcgac
      901 ccaccgttcc cgcgcccggc ccaggcctac ctggacgagt gggcatcgcc ggagaagggc
      961 tggctgcgca agttctaccc gtccggcatc gacgagccgc actacgacat cacgcccccg
     1021 gtggagaagg cggtgctatg gctgaaggat ctcaggcggc gcgagttcgt cgggacggag
     1081 tctcggctca acacgttgtt cgagctgctg cgccagatgg tgttcggcgc ggacaccgac
     1141 ccggagagtc agctcgccga tctgcgtcgg cgccgggccg agctggacga gcagatcgcc
     1201 cgggccgagc gcggcgaggt ggcgctgctc gacgcgacca gtcagcgcga ccgctactac
     1261 cagttcgccc gcaacgcccg tgagctgctg gccgacttcc gtgaggtcga ggagaacttt
     1321 cgaatactcg accgacgact ccgcgagcag atcgccggct ggtcgggttc gaagggcgca
     1381 ctgctcgatg aggccctgaa cagccgcaac tcgatcaccg agtccgatca ggggcgaagt
     1441 ttccaggccc tgttcgactt cctgctgtcc cgccagcgcc aggccgagct gagcgagctc
     1501 ctcgaccggc tcggccagat cagcgagctc gacgagatcg acccgcgcct gcgccatgtc
     1561 cacttcgact ggatcgacgc cggcgagcgc acccaaggca cggtccggct gttgtccgag
     1621 cagctgcgcc ggttcctcga cgatcaggtc tggctggaga accggcgcat cttcgatctg
     1681 ctgcacagca tcgaggcgac cgcggtcgag cttcgcaacc ggccgatcgg cctggccatg
     1741 gaactgcccg ggatgggggt cgaggtgaac ctgccgttcg agcggccgct ctacacgccg
     1801 atcgcaccgg tgagcctgga atcggacgcg gtcgaggtcg gcagcaccga cttcgacgcc
     1861 accgcgctcc tcgatcagct gtacgtcgac cgcgacgagc tggcccgcac cgtccggtcc
     1921 agcctcagtg ggcgcagcca ggtcgggctt gccccactga tcgccgacca tccgctgcac
     1981 cagggactcg ccgagctgat cggctacctt tcgctgtccg atccggcgtt cgacgtcgtg
     2041 ttcgacgaag acgaccgcga ccggatcggc tggaccaccg acgagaccga acgggtcgcc
     2101 gacacgccgt cggtcacctt cgcacgacgc accacgggag gcacctcgtg agccaggcaa
     2161 cgctggaagc cagcgagctg acggcggtgg tcgtccagct gatgaagggc ccggtctatc
     2221 gcgacacgca cgagaagatc tggcaccagt tgctcgccgt gcgaaaccag gcgagcgact
     2281 acgtggccgt tctcgggctg caggtcatga tcgacgaggc cgaggggtac gcctttctgc
     2341 gcgcccggcc ggtcgaggag accggtgagg atctcccgcg gctcgtgcca cgtcgcgcgt
     2401 tgtcctaccc ggtcagtctg ctgctcgcct tgctgcgcaa gaagctggcg gagttcgatg
     2461 cggtcagctc cgacagtcga ctggtgatca gccgcgagca gctggtcgag ctcatccgta
     2521 ccttcctgcc ggacacacca aacgaggcca ggctggtcgc aaccatcgac acccacatcg
     2581 gcaaggtggt cgacctcggc ttcctgcggc cgctacgcgg ccaaccggac cagttcgagg
     2641 tgcgccggat tctcaaggcg tacgtcgacg gccagtggct gtcgcagctc gagtccagac
     2701 tgcgcggcta ccgcgaccag ctcgccggcg aggaggcccg atgaccgacg cgttgttcag
     2761 caccctcgag gttgaggacg ccgacccgag ccggacgccg ggcacacggt tgcggcgact
     2821 cgaggtctac aactggggca cgttccacaa ccgggtctgg accttcgacg tcgcgggccg
     2881 caacgcgctg ctcaccggcg acatcgggtc cggcaagtcg acgctcgtcg acgccgtcac
     2941 gaccctcttg ctgccgtcgc accggatctc gtacaacaag gcggccggcg ccgacacccg
     3001 ggagcgcgac ctgcggtcgt acgtcctcgg tcactatcgg tccgagcgaa acgagatcac
     3061 cggtgcctcc cggcccgtgg cgctgcgcga tacccggtcg tactcggtgc tgctcggagt
     3121 cttcggcaac gctgacttcg acaccacgac gaccctggcg caggtgttct ggacccggga
     3181 cggccagtcg ggccagcccg atcggttcta cgtcgtcgcc gacggtgacc tctcgatcgc
     3241 cgagcacttc gccggcttcg gcaccgacgt gacgcagctg cgcaaccggc tgcagcggat
     3301 gggcctgaag gtacacaagg cgtttcccga atacggccgg gaactgcggc ggcggctcgg
     3361 catcgagtcc gagcaggcgc tggagctgtt ccaccagacg gtgtcgatga aggcggtcga
     3421 caacctcaac gacttcgtcc ggcatcacat gctggagccg ttcgacgtcg ggcaccagat
     3481 cgaggcgctg gtgcggcatt tcgaggatct gacccgggca catgaggcgg tcgtcagggc
     3541 aaaggcacag ctggagctgc tcgggccgct gatcgccgac ctcgacaccc acgacggcct
     3601 ggcagccgag gtcgccgatc tcgggcacca gcgggccgca ctgccctatt tcctggcgcg
     3661 gcgccgggag cgtctgctcg ccgcggagct cgccgagacc gagcagcggc gtgccgccct
     3721 cgcggccgag gagacgacta ccaggg
//
LOCUS       DATBFZ010000270         9412 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_6327704, whole genome shotgun sequence.
ACCESSION   DATBFZ010000270 DATBFZ010000000
VERSION     DATBFZ010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 9412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9412
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6327704"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1974
                     /locus_tag="VH419_08300"
     CDS             <1..1974
                     /locus_tag="VH419_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012920994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-like protein EF-G2"
                     /protein_id="HEY7043653.1"
                     /translation="QRSIGLSLVPLIVGGGDATTKINLLDTPGYADFVGDLRAGLRAA
                     DCALFVISANDGVDGATKALWQECEAVGMPRSVVISKLNHQRADFDAVVGAAQAAFGD
                     KVLPLYIPVQHSGSTGAEEITDDLTALLSQTVSDYSEFDSKGRVVRQPNDDEQLLIEE
                     HRSTLIEGIIEESEDEGLMDRYMGGEDIDFDLLVDDLETAVARGSFFPVIPVCAQTGL
                     GLDELVEIITQAFPSPSEHTFPDVYTTAGKTVDHLSCDPSGPLVAEIVKTTSDPYVGR
                     ISLARVFSGTIRPDATVHVSGHFTEFYGTDRGHEDHDEDERIGALSSPLGKTQRLVDS
                     CIAGDICAIAKLSRAETGDSLSDKDQPLVMEPWSMPEPLLPIALVAHAKADEDKLGQA
                     LQRLSAEDPTVRIEHQVETSQLILWCMGEAHADVLLDRLSSRYGVQVDQVDVRVPLRE
                     TFAAQGKGHGRHVKQSGGHGQYAICDIEVEPLGTGEGFEFVDKVVGGSVPRQFIPSVE
                     KGVRAQLEKGLSAGYPVVDLRVTLYDGKAHSVDSSDMAFQTAGALALKEAAGSAGVSL
                     LEPIDEIEVLIDDDYVGAVMGDLSTRRARVLGTEPVGTGRTLVKAETPQLELISYATE
                     LRSLSHGTGTFSRSFVRYEPLPSHVASKVVEPS"
     gene            2081..3028
                     /locus_tag="VH419_08305"
     CDS             2081..3028
                     /locus_tag="VH419_08305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043654.1"
                     /translation="MGLILVMVGGCGGSANPLHGPDATPHARRAAATTVDVCRLVSSR
                     TAATVLDRPLLVIGRTVGAPRDPTVSCVIGPRFDEPVVTVSLAPVPVALDVFDEAYGE
                     QAGGNPLPVRHLGRAAYIRTEGAQRALHVFVHQAILSVLVALAPTGSPDRITTQQLVD
                     LTRNAVRNIPANPHIAAHTAPDRCVRISPAVLAETLGRAPTLDAGLVYSSGSLQCSWG
                     SQPGSVTVTATDDPATIQRFRGDHDLAEYVAVPGVLARSEGRAYSSPQVPGDLIVLVG
                     ADELLITMEVVPAAGYADAAIDTTKSERTLAAAVLADLT"
     gene            complement(3377..3934)
                     /locus_tag="VH419_08310"
     CDS             complement(3377..3934)
                     /locus_tag="VH419_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016456547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEY7043655.1"
                     /translation="MTVFEPQEGVGEPDPFQRLWTPHRLAYIKGENKPSQDPDAENGC
                     PFCMIPTMTDEDGLLVRRGETAYAVLNLYPYNPGHLMICPYRHVPDYTELDRPEVVEI
                     AELTRQAMSAVREASAAHGFNIGMNQGNVAGAGIAEHLHQHVVPRWGGDTNFMPVVGR
                     TKVLPQLLRDTRELLADAWTKVAGD"
     gene            complement(3960..5942)
                     /gene="thrS"
                     /locus_tag="VH419_08315"
     CDS             complement(3960..5942)
                     /gene="thrS"
                     /locus_tag="VH419_08315"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEY7043656.1"
                     /translation="MSDLRVTVVGEADRAERTVTTGTKAWELFRDEPEVIAARVDGEL
                     RDLSHELADGAEVTPVAIDSPDGRDILRHSTAHVLAQAVQQLFPDAKLGIGPPITDGF
                     YYDFDVPAPFHPDDLDTIEARMRRIVKEGQRFSRRPVADDEARRELVDEPYKLELIGL
                     KGSGAASDAEGADVEVGAGELTIYDNLKRDGSLAWKDLCRGPHLPTTKRIPAFKLMRS
                     AAAYWRGDEKNPQLQRIYGTAWESKEALEDHLHRLEEAERRDHRRIGRDLDLFSFPDE
                     LGSGLVVFHPKGGVIKRVMEDYVRQRHIDEGFLYVGTPHISKQGLFETSGHLPYYAET
                     MYPPMEMEGSNYYLKAMNCPMHNLIFRSRGRSYRELPLRLFEFGSVYRLEKSGVVHGL
                     TRVRGMTQDDSHSYCTAEQAPDEIKHLLGFVLSLLKDFGLDDFYLELSTRDDDSDKFV
                     GSDEEWAAATKILENVALESGLDLVPDPGGAAYYGPKISVQARDAIGRTWQMSTIQYD
                     FNQPRGFELEYQAADGSRQRPVMIHSAKFGSIERFFGVLVEHYAGAFPPWLAPVQVVG
                     IPIAERHHPYLAEVADRLRAAGVRVEVDESDDRMQKKIRNAQLHKVPFMVIAGDKDVE
                     EGAVSFRYRSGEQDNGVAVSEAIARIVKAVESRVQV"
     gene            complement(6045..6533)
                     /locus_tag="VH419_08320"
     CDS             complement(6045..6533)
                     /locus_tag="VH419_08320"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY7043657.1"
                     /translation="MARRSARRLEAQRAFTERMGSIMFDTGLARMPSRVFAALLASDE
                     GQMTAAELADQLKASPAAISGAVRYLAQIGLVARERESGSRRDHFVLHEDVFYELMAQ
                     DVRSITRWESGLRDGIANLGANTPAGQRITRTLDFFEFMRAELTDMMERWQAERAGQS
                     GS"
     gene            6620..7534
                     /locus_tag="VH419_08325"
     CDS             6620..7534
                     /locus_tag="VH419_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY7043658.1"
                     /translation="MAQAIDVESLQKRFGPTVALDGLDLSVRSGEVHGFLGPNGAGKS
                     TTIRILLGLLRADGGHARLLGGDPWAQAASLHNRLAYVPGEVSLWPNLTGGEVIDLLG
                     RLRGNLDQRKRTDLLDRFELDPRKKSRTYSKGNRQKVALVAALSSDVELLVLDEPTSG
                     LDPLMEAVFQEAIREERAAGRTVLLSSHILAEVEALCDRVSIIRSGKLLESGTLTDLR
                     KLTRTSVTAETERRSSDLDTLPGIRNTAWTDHRVTFDVDSEHLDGAVRHLSSLGVRSL
                     VSHPPTLEELFLRAYGEEPATTQGGVRQ"
     gene            7597..9120
                     /locus_tag="VH419_08330"
     CDS             7597..9120
                     /locus_tag="VH419_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043659.1"
                     /translation="MRLPVWVLSIVGLIVASANAVQTVYATPHDRDLYANSVGESTGA
                     IAMAGPPIALHTLAGITVFETSATALVSIALMAIFLTVRHTRAEEEAGRTELLRAGRL
                     GRLAPVAATGIAVGGGSVVVGAAIALSLVGLGLPTSGSWLFGASIACFGLVMTGVALL
                     CAQIFEHARTAIGTGSAILGAAFTIRAVGDVSTSALSWLSPMGWSQATHPYEANRWWP
                     LVLPVVATAALVVASAALVERRDMGAGLVAPRLGPPSAPPRLASSWGLALRLQRTSAL
                     WWAFGLFVGGIAFGSVAGSVDDLIGDRQDLKDALVPEGGDIVDGYLTTSLLILALVAG
                     GFTVSSVLRLRSEETSGRAEPLLATGLSRLRWATGTLVFTAVGTVAVLAAGGVGLGLT
                     GAIVTRDAGLFGRTVAGAVIYVPAALVLAAFAVLLEGWLPQATPVAWAVLAGCFVIGY
                     LGQLLEFPQWLTNASPYTHVPKVLIDGFTAAAPLVLLLIAALLGASGIGGLLRRDLG"
     gene            complement(9121..>9412)
                     /locus_tag="VH419_08335"
     CDS             complement(9121..>9412)
                     /locus_tag="VH419_08335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043660.1"
                     /translation="VPEPRTYQELFDADVAVRDPGNAWQGLRAWSAEERTAIELGVRA
                     TVVDVVTCRALTIVEVDFTNPPAWPDHCPSRSTFVHHLEAGRSRQVSIYYHA"
BASE COUNT         1433 a         3242 c         3139 g         1598 t
ORIGIN      
        1 cagcgatcga tcggcctctc cctcgtcccg ctgatcgtcg gcggcggcga cgccacgacg
       61 aagatcaacc tgctcgacac tcccgggtac gccgacttcg tgggagacct gcgtgcgggt
      121 ctccgcgccg ccgactgcgc gttgttcgtg atctccgcca acgacggcgt cgacggcgcc
      181 accaaggcgc tgtggcagga gtgcgaggcc gtcggtatgc cacgcagtgt ggtgatcagc
      241 aagctcaacc accagcgcgc cgacttcgac gccgtcgtcg gggcggccca ggccgccttc
      301 ggtgacaagg tgctgccgct gtacatcccg gtccagcact ccggctcgac cggtgcggag
      361 gagatcaccg acgatctcac cgctttgttg tcgcagaccg tgtccgacta ctcggagttc
      421 gactcgaagg gccgcgtcgt acgccagccg aacgacgacg agcagctgct catcgaggag
      481 caccggagca cgctcatcga gggcatcatc gaggagtccg aggacgaagg cctgatggac
      541 cgctacatgg gtggcgagga catcgacttc gacctgctcg tcgacgacct cgagaccgcg
      601 gtggcgcgtg gctccttctt cccggtcatt cccgtctgcg ctcagaccgg cctcggtctc
      661 gacgagcttg tcgagatcat cacgcaggcg ttcccgtccc cgtcggagca cacgttcccc
      721 gacgtctaca ccaccgccgg caagaccgtc gaccatctgt cgtgcgaccc ctccggaccg
      781 cttgtcgccg agatcgtcaa gacgacgtcc gacccttacg tcgggcggat cagcctggcc
      841 cgggtcttct cgggaacgat ccgccccgac gccaccgtgc acgtgtccgg ccacttcacc
      901 gagttctacg gcaccgatcg cggacacgag gaccacgacg aggacgagcg catcggtgcg
      961 ctctcgtcgc cgctcggcaa gacccagcgc ctcgtcgact cgtgcatcgc cggcgacatc
     1021 tgcgcgatcg ccaagctgtc ccgggcggag accggcgact cgctgagcga caaggaccag
     1081 ccgttggtga tggagccctg gtcgatgcct gaaccgctcc tgccgatcgc cttggtggcg
     1141 cacgcgaagg ccgacgagga caagctcggg caggcgctgc agcgactgtc ggccgaggac
     1201 cccacggtcc ggatcgagca ccaggtggag acgtcgcagc tgatcttgtg gtgcatggga
     1261 gaggcccacg ccgacgtgct gctcgaccga ctgtccagca ggtacggcgt ccaggtcgac
     1321 caggtggacg tccgggtgcc actgcgggag acgttcgcgg cgcagggcaa gggccacggg
     1381 cggcacgtca agcagagcgg tggccacggc cagtacgcca tctgtgacat cgaggtcgag
     1441 ccgctgggca ccggggaggg cttcgagttc gtcgacaagg tggtgggcgg ttccgtcccc
     1501 cgccagttca tcccgagcgt ggagaagggg gtccgggcgc agctcgagaa gggtttgtcc
     1561 gccggctatc ccgttgtcga cctccgggtc acgttgtacg acggcaaggc gcacagcgtc
     1621 gactccagcg acatggcctt ccagacggcg ggggcgctcg cgctgaagga ggcagccggc
     1681 tccgccggtg tctcgctgct cgagccgatc gacgagatcg aggtcctgat cgacgacgac
     1741 tacgtcggtg cggtgatggg cgacctgtcg acccggaggg cccgcgtgct cgggaccgaa
     1801 ccggtgggca ctgggcgcac cctcgtcaag gcggagacgc cgcagctcga gctgatcagc
     1861 tacgccaccg agctgcggtc cctcagccac ggaaccggga cgttctcgcg cagcttcgtc
     1921 cgctacgaac cacttccctc ccacgtggcg tcgaaggtgg tcgagccctc ctgaacgctt
     1981 cgggggaccc gcgaattgcg ggaaagcgca tgccgacacg ccggtgacgg cacgatgttg
     2041 cccggggttc ccgcaattgc gcggagccgt tgcctgtgtt gtgggtctga tcctcgtcat
     2101 ggtcggcggc tgtggcgggt cggcgaatcc gctgcacgga ccggacgcga cgccgcatgc
     2161 ccgtcgggcc gcggccacga ccgtggacgt ctgtcgactg gtctcgtcgc ggaccgccgc
     2221 aaccgttctc gatcggccgc tgctggtgat cggccggacc gtgggagcgc cacgcgaccc
     2281 cacggtctcg tgcgtgatcg ggccacgctt cgacgagccg gtcgtgaccg tgtcgcttgc
     2341 gcccgtgccg gtggccctcg atgtgttcga cgaggcgtac ggcgagcaag ccggtggtaa
     2401 cccgttaccg gttcggcatc tcggcagggc ggcgtacatc cgcacagaag gcgcgcagcg
     2461 cgcactccac gtcttcgtgc accaagcgat cctgtccgtc ttggtcgccc tcgcaccgac
     2521 cggaagtccg gaccggatca cgacccagca gctcgtcgac ctgacccgca acgccgtgcg
     2581 gaacatcccc gccaaccctc acatcgccgc ccacacagct cccgaccgct gtgtccgtat
     2641 cagtccggcc gtgctcgccg agacgctggg acgggcaccg actctcgatg caggcctggt
     2701 ctactcgtcg gggtcgctgc agtgctcgtg gggcagccaa cccggctcgg tcaccgtgac
     2761 cgcgaccgac gaccctgcga cgatccaacg gttccgtggt gaccacgacc tcgcggagta
     2821 cgtcgcggtc ccgggcgtgc tggcgcggtc ggagggccgg gcgtactcca gtccgcaggt
     2881 tccgggagac ctgatcgtgc tggtcggtgc ggacgagctg ctcatcacga tggaagtcgt
     2941 ccctgccgcc ggctacgccg acgcggcgat cgacacgacg aagagcgaac ggacgctggc
     3001 cgccgccgtc ctcgcggacc tcacctgaca cgaccgtttc ctgccatcgc gtgacggttt
     3061 gcctcggtag ccatccaaac ggacggctag ccaggcagat ctgccctggc aggggtggac
     3121 ttgcctgggt agccatgcaa gccgccctcg gcgaccgacg gatgtccgaa aaccgccagt
     3181 cacacacccc caggccaagg ggacggaagc cggccggccc gcgcagtgag cccgaacgtc
     3241 ccccgctcct cagagctgat ccaacgcaac tgatgacgac cgaacgccga gcgaccaggc
     3301 gtcacccacc gcgaccaggc ccccccgaag gggcatcagc ggtcgccgcc tggcaccggg
     3361 ctggccgcca gggcgcctag tcccccgcga ccttcgtcca cgcgtcggcg agcagctccc
     3421 tggtgtccct cagcagctgc ggcagcacct tggtccggcc gaccaccggc atgaagttcg
     3481 tgtcgcctcc ccaacgcggg acgacgtgct gatggaggtg ctcggcgatg ccggcaccgg
     3541 caacgttccc ctggttcatg ccgatgttga aaccgtgcgc agccgaggcc tcccggactg
     3601 cggacatcgc ctgccgcgtc agctcggcga tctcgaccac ctcgggtcga tcgagctctg
     3661 tgtagtcggg cacgtggcgg tacgggcaga tcatcaggtg gccggggttg taagggtaga
     3721 gattcagcac ggcgtacgcc gtctctcccc tgcgtacgag cagcccgtct tcgtcggtca
     3781 tcgtcgggat catgcagaac gggcagccgt tctcggcgtc cgggtcttgc gacggcttgt
     3841 tctcgccctt gatgtaggcg agccggtgcg gtgtccacag ccgctggaac gggtccggct
     3901 cgccgacacc ttcctgcggc tcgaagacgg tcatcggcgc accgacgtcg cggtcgccgt
     3961 cacacctgca cgcggctctc gacggccttg acgatgcgcg cgatcgcctc gctcacggcc
     4021 acgccgttgt cctgttcgcc actgcggtag cggaacgaga ctgcgccctc ctcgacgtcc
     4081 ttgtcaccgg cgatgaccat gaacggaacc ttgtgcagct gggcgttgcg gatcttcttc
     4141 tgcatccggt cgtcggactc gtcgacctcg acccggacgc ccgccgcgcg caacctgtcg
     4201 gcgacctccg caaggtacgg atggtgacgt tccgcgatcg gaatcccgac gacctgcacc
     4261 ggtgcgagcc agggcgggaa cgctccggcg tagtgctcga cgaggacgcc gaagaagcgc
     4321 tcgatcgaac cgaacttcgc cgagtgaatc atgacgggcc gctgccgtga accgtcggcc
     4381 gcctggtact cgagctcgaa gccacgcggc tggttgaagt cgtactggat cgtcgacatc
     4441 tgccaagtcc gcccgatcgc gtcgcgcgcc tggacggaga tcttcgggcc gtagtacgcc
     4501 gcaccgcccg ggtccggcac gaggtccagg cctgactcga gtgcgacgtt ctccaggatc
     4561 ttggtggccg ccgcccactc ctcgtcgctg cccacgaact tgtccgagtc gtcgtcgcga
     4621 gtggagagct cgaggtagaa gtcgtcgagc ccgaagtcct tgagaaggct cagcacgaag
     4681 ccgagcaggt gtttgatctc gtcgggcgcc tgctcggcgg tgcagtagct gtgtgagtcg
     4741 tcctgggtca ttcctcgcac ccgggtgagg ccgtgcacga ccccggactt ctccagccga
     4801 tagaccgaac cgaactcgaa gagccgcagc gggagctccc ggtacgaccg cccacgcgac
     4861 ctgaagatca ggttgtgcat cgggcagttc atcgccttga ggtagtagtt gctgccctcc
     4921 atctccatgg gcgggtacat cgtctccgcg tagtacggca gatggcccga tgtctcgaac
     4981 agaccctgct tgctgatgtg cggcgtaccg acgtacagga agccctcgtc gatgtggcgc
     5041 tggcggacgt agtcctccat gacccgcttg atgaccccac ccttcgggtg gaacacgacg
     5101 aggcccgagc cgagctcgtc cgggaagctg aacaggtcca ggtcacgccc gatccggcgg
     5161 tggtcgcgac gctcggcctc ctcgagccgg tgcaggtggt cctcgagagc ctccttggac
     5221 tcccaggcgg tgccgtagat gcgctgcagc tgcgggttct tctcgtcgcc gcgccagtac
     5281 gccgccgccg accgcatcag cttgaacgca gggatgcgct tcgtggtggg caggtgtgga
     5341 ccgcggcaca ggtctttcca ggccagcgac ccgtcccgct tgaggttgtc gtagatcgtg
     5401 agctctcccg cgccgacctc gacgtcggca ccctcggcat ccgaggcggc accggagccc
     5461 ttcaacccga tgagctcgag cttgtacggc tcgtcgacga gctcacgtcg tgcctcgtcg
     5521 tcggcgaccg ggcgtctgct gaaacgctgc ccctccttga cgatcctgcg catccgagcc
     5581 tcgatcgtgt cgaggtcgtc ggggtggaac ggcgccggca cgtcgaagtc gtagtagaag
     5641 ccgtcggtga tcggtgggcc gatcccgagc ttggcgtcgg ggaacagctg ttggacggcc
     5701 tgggccagca cgtgcgcagt cgagtgccgg aggatgtcgc gcccgtccgg tgagtcgatc
     5761 gccaccggcg tgacctcggc accgtcagca agctcatgcg acaggtcgcg cagctcaccg
     5821 tcgacgcgcg ccgcgatcac ctcgggttcg tcgcggaaca gctcccacgc cttcgtgccc
     5881 gtcgtcaccg tccgctcagc acgatcggct tcgcccacga cggtgacacg caggtcggac
     5941 acgggaacgc tccttcgaga tctcggctgg gttcggcgta cgagcagatg cgtcgatcgt
     6001 atcgagcgcg ccttggccca gttgcgttcc gtcccatcgt caggtcagga gccggactgg
     6061 ccggcgcgtt cggcttgcca gcgctccatc atgtcggtga gctcggcgcg catgaactcg
     6121 aagaagtcga gggtcctcgt gatccgctgc cccgcagggg tgttcgcacc gagattggcg
     6181 atgccgtcgc ggaggccgga ctcccatcgg gtgatggatc tgacgtcttg cgccatcagc
     6241 tcgtagaaga cgtcctcatg caggacgaaa tggtcgcggc gcgatccact ctcgcgctcg
     6301 cgtgcgacca gcccgatctg tgcgagatag cgcaccgccc ctgagatggc ggcagggctc
     6361 gccttgagct ggtccgcgag ctcggcagca gtcatctggc cttcgtcgga cgccaacagc
     6421 gcggcgaaga cgcgtgacgg cattcgcgcc agcccggtgt cgaacatgat cgagcccatc
     6481 cgttcggtga acgctcgttg ggcctccagc cgccgggccg accttcgtgc cacgcgattt
     6541 ccttccggtg agaaccctca caacattcag tcaagatagt ggtttcagtt gtttgtgaaa
     6601 tgagtgtacg ttgaagagca tggcccaggc aatcgacgtt gaaagtctgc agaagcgctt
     6661 cggccccacc gtcgcactcg acgggctcga cctgtcggtt cgcagtgggg aggtccacgg
     6721 gttcctcgga cccaatggcg ccggcaagtc cacgacgatc cgcatcctgc tcgggctgtt
     6781 acgcgcggac ggaggtcacg cccggctact cggcggtgat ccgtgggcgc aggcggcctc
     6841 gctgcacaac cggctcgcgt acgttccagg tgaggtctcc ttgtggccga atctgaccgg
     6901 cggtgaggtc atcgacctgc tcggccgact ccgaggaaac ctcgaccagc ggaaacggac
     6961 cgacctgctc gaccggttcg agctcgaccc acggaagaag agccggacgt actccaaggg
     7021 aaaccggcag aaggtcgcgc tggtggccgc actctcgtcc gacgtcgagc tgctggtgct
     7081 cgacgagccc acgtccggcc tcgacccgct gatggaggcg gtgttccagg aggccatccg
     7141 tgaagagcgt gccgctgggc gaacggtcct cctgtccagc cacatcctgg ccgaggtcga
     7201 ggcgttgtgc gatcgcgtca gcatcatccg ctccggaaag ctgctcgagt ccggcacact
     7261 gaccgacctt cgcaagctca cccgcacgtc cgtcacggcg gagaccgaac gccgctcctc
     7321 cgacctggac actcttcccg gcatacgcaa cacggcgtgg accgatcacc gagtgacgtt
     7381 cgacgtcgac tccgagcacc tcgacggtgc ggtgcgtcac ctttccagtt tgggcgtgcg
     7441 ttcgctggtc agccacccac cgaccttgga ggagttgttc ctgcgggcgt acggcgagga
     7501 gccggccacc actcagggcg gcgtacgcca gtgaacgctc gttcgaactt ggccggcacg
     7561 ttcacggtgt tgcgattcat cctgcgacgc gatcgagtgc gtcttccggt ttgggtgctg
     7621 tcgatcgttg ggctcatcgt cgcgtcggcg aacgccgtcc agaccgtgta cgccaccccg
     7681 cacgaccgcg acctctacgc gaactccgtg ggcgagagca ccggcgcgat cgcgatggcc
     7741 ggtccgccga tcgcgctcca cacactcgca ggaatcaccg tgttcgagac cagcgcgacg
     7801 gcccttgtct cgatcgcgct gatggcgatc ttcctcacgg tccggcacac cagagccgag
     7861 gaggaagccg gccgcaccga gctgctccgc gccggtcggc tcggtcggct tgcgccagtg
     7921 gccgcaaccg gtatcgccgt cggtggcggc agcgtggtgg tgggcgcggc catcgcgctc
     7981 tcgttggtcg gcctgggcct gcccaccagc gggagttggc tgttcggtgc ttcgatcgca
     8041 tgtttcggtc tggtcatgac cggcgtcgcg ctgctctgcg cgcagatctt cgagcacgca
     8101 cggacagcga tcggaacggg ttcggcgatc cttggcgcag cgttcaccat ccgcgccgtc
     8161 ggcgacgtct cgacctcagc gctgtcctgg ctttccccga tgggttggtc gcaggcgacg
     8221 cacccctatg aggcgaaccg ctggtggccg cttgtgctgc cggtggttgc gacagccgcg
     8281 ctcgtcgtgg cgtccgcggc gctcgtcgaa cgacgcgaca tgggcgcagg tctcgtcgct
     8341 ccccgcctcg gtcccccgtc ggcaccgcca cggttggcaa gctcgtgggg gctcgccctc
     8401 cgcctccagc ggacgtcggc gctgtggtgg gccttcggcc tgttcgtcgg cggtatcgcc
     8461 ttcggttcgg tcgcggggag cgtggacgac ctgatcgggg atcgtcagga cctgaaggat
     8521 gcgctggtcc ccgaaggcgg agacatcgtc gacggctacc tcacgacatc actgctcatc
     8581 ctcgcgctgg tcgccggcgg attcaccgtc tcgtcggtcc tgcggctgcg cagcgaagag
     8641 acgtcgggtc gggccgagcc gctcctcgcc accgggctca gccggctgcg ctgggcgacg
     8701 gggacgctgg tcttcacggc ggtgggcacc gtcgcagtcc tcgctgccgg cggagtgggc
     8761 ctcggcctca caggcgccat cgtcacacgg gacgccggac tcttcggacg gacggtggcc
     8821 ggcgccgtca tctacgtgcc ggccgcgctt gtgctggcgg ccttcgcagt cctgctggag
     8881 ggctggctgc cgcaggcgac acccgtcgcg tgggccgtgc tcgccggctg cttcgtgatc
     8941 ggctacctcg gccagctgct cgagttcccg caatggctga cgaacgcgtc gccatacacg
     9001 cacgtcccaa aggtcctcat cgatggcttc accgcggcgg cgccgcttgt cctgttgctc
     9061 atcgccgcgt tgctcggtgc gagcgggatc ggcggtctgc tgcgccggga cctggggtga
     9121 tcatgcgtgg tagtagatcg agacctggcg tgaccgtccc gcctcgaggt ggtgcacgaa
     9181 cgtgctgcgc gacgggcaat ggtcgggcca cgccggcggg ttcgtgaagt ccacctcgac
     9241 gatcgtcagt gcacggcacg tcaccacgtc gaccacggtc gcgcgtacgc cgagttcgat
     9301 ggccgtccgc tcctctgccg accacgcgcg aaggccctgc caggcgtttc cgggatcacg
     9361 aaccgcgaca tccgcatcga acagctcctg atacgtccgg ggctccggca cc
//
LOCUS       DATBFZ010000271        18010 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_181472, whole genome shotgun sequence.
ACCESSION   DATBFZ010000271 DATBFZ010000000
VERSION     DATBFZ010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 18010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18010
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181472"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="VH419_08340"
     CDS             complement(<1..489)
                     /locus_tag="VH419_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012918648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HEY7043661.1"
                     /translation="MTVPPLVPHPTTMITVAPTGAESAKEDVPALPTTIEELAETAVR
                     CEAAGAAMIHLHIRDTAHRPTLEIARLTEAVAAVREATSLVVQLSTGGSVHDPLEQRL
                     KVLDAEPDSCSLTMGTTNFADDVFLNPWPFIKDLYQLSQEREVVPEFELFDLGHVHAL
                     SRL"
     gene            557..1003
                     /gene="dtd"
                     /locus_tag="VH419_08345"
     CDS             557..1003
                     /gene="dtd"
                     /locus_tag="VH419_08345"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015071415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HEY7043662.1"
                     /translation="MRAVVQRVSQASVCVDGTVTGSFDEAGLVVLVGVTHDDTPAQAA
                     ALATKIWEMRIMRDELSAADLAAPVLVVSQFTLYADTRKGRRPSWSAAAPGSVSEPLY
                     EEFCRQLGALGATVHTGTFGAQMQVRIVNDGPVTVIVDTATPGRRR"
     gene            1130..2332
                     /locus_tag="VH419_08350"
     CDS             1130..2332
                     /locus_tag="VH419_08350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEY7043663.1"
                     /translation="MTDTTRYHEDGGSPEPSSGVVDPARRSSAASGLLSVVIGAPRQR
                     SRGGRHKRKRKTVIGRHPVVLALALVLTLVAGSAGGYLWWLNHQLGNIQRVDAGISEN
                     PAIDGHEGGKPLNILLLGADNGNAQTESVADDLADGKWTPFEHRSDTMMVVHIPADRK
                     SVQLVSIPRDTWVPIDGYPYTDGHGKINSAFAYGGPSLARQTVEDLTGIHIDHLAIID
                     WAGFKDLTSALGGVRVYVPDTFYDDKQDITWEKGWHDLEGKDALSYVRTRHGLANGDF
                     DRIKRQQNFLRATMGQLLSSGTTHNVVKFTKVVGAMTKYLVVDNTWDNGEIKSLALSM
                     RNLHSHDVDFLTAPFGSYDRSPDGQSIVRLAPKKSTALFAAVEDDDVQHYLEENPKSA
                     LAGAHSIN"
     gene            2441..3592
                     /locus_tag="VH419_08355"
     CDS             2441..3592
                     /locus_tag="VH419_08355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEY7043664.1"
                     /translation="MDEKPGDEAPAATPPSPPPPDDSEQPDASTPAGEPPAKKRRWVM
                     RHKALTAVAVVLVLVAGCSGGYLWWADRQLSSIPRIDIHIHTPPEKNHHESNKDLNIL
                     LLGADHGAVGQSVAEDLADGKWTPFKHLSDTIIVLHIPADRKSVQMVSIPRDTWVHID
                     GYPYGNHHAKINAAFSYGGPELAYKTVQQFTGLTIDHVAVIDWVGFRELTEALGGVRI
                     YIPETFTDYKQDVTWEKGWHTLEGSDALKYVRTRHGLANGDFDRMARQQNFLRAIMAK
                     MLSSSTTHNPLRFSKVLASLSSYLTIDSTWDNDELRSLAWSLRNLHAGDVDFFTAPFG
                     SYATIDGQSVVRLDMPQFRFLMRSIDDDHVEKYVARYPGNHLASQTNIN"
     gene            complement(3585..3959)
                     /locus_tag="VH419_08360"
     CDS             complement(3585..3959)
                     /locus_tag="VH419_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008360694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannose isomerase type II C-terminal
                     cupin domain"
                     /protein_id="HEY7043665.1"
                     /translation="MAIERDERPWGYYEVVDAGPGFRVKRICVRPRQRLSYQRHSRRA
                     EHWFVVTGHGVATVDGVLHEVTVGSTVDVPLGAAHRVANDGETDLIFVEVQTGGYFGE
                     DDIERLQDDYGRPTSGPRLQIS"
     gene            complement(4161..4457)
                     /locus_tag="VH419_08365"
     CDS             complement(4161..4457)
                     /locus_tag="VH419_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016644785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1416 domain-containing protein"
                     /protein_id="HEY7043666.1"
                     /translation="MCGATAGGPSLEGVDVAKESIIQGVVVHDDHPVSGAYVRLLDRL
                     GEFTAEVPTSATGQFRFFAAPGDWTLRTLAPKVEPVDQRVTAARGDVAEVRIAV"
     gene            complement(4458..5318)
                     /locus_tag="VH419_08370"
     CDS             complement(4458..5318)
                     /locus_tag="VH419_08370"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017936568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEY7043667.1"
                     /translation="MSRDAVLVDADWVQSRLGDDGQDRIVLVEVDEDTTAYDKGHIKG
                     ALKIDWKADLQDPVRRDFVDKEQFEQLMSARGIANDDTVVLYGGNNNWFAAYAYWYFK
                     LYGHEKALLLDGGRKKWELDSLELTEEVATREPTSYTAKDPDLDIRAFRDEVVAAIGT
                     KNLVDVRSPDEFAGRLLAPAHLPQEQAQRGGHVPTAVNIPWSKAANDDGTFRSDDELR
                     TLYSEAGVEFGAKDTIAYCRIGERSAHTWFVLHEILDEPNVKNYDGSWTEYGSLVGVP
                     VAVGDDPGEA"
     gene            5673..6056
                     /locus_tag="VH419_08375"
     CDS             5673..6056
                     /locus_tag="VH419_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY7043668.1"
                     /translation="MTVQLNHTIVNCRDKRAAADFFADILGVGPATEFGPFIVLELSN
                     DVSLDFLDQAPEVHAQHYAFLVDETEFDEILGRIRDRGLPYWADPFQRQPERINTNDG
                     GRGVYWQDPNGHMLEIITRPYGSEG"
     gene            complement(6386..6700)
                     /locus_tag="VH419_08380"
     CDS             complement(6386..6700)
                     /locus_tag="VH419_08380"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEY7043669.1"
                     /translation="MPDPVVATTADGTAPDSGPPVVTVRYWAAARAAAGRVEDTVEGV
                     QTVQAALAAVHALHDDARFRDVLSVSSLVLGDQPVHRAHLADVPVTAGDVIEVLPPFA
                     GG"
     gene            6785..7534
                     /locus_tag="VH419_08385"
     CDS             6785..7534
                     /locus_tag="VH419_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008360662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY7043670.1"
                     /translation="MSNLLLLTNALQPSAELLPALALLPHHVRILPAEPTVLVEAPDC
                     DAVLVDGRHDLASVRSLCRLIRTTGVDVPVLVIVTEGGLTVVAADWGVDDVLLSTAGP
                     AEVEARLRLSAGRLALARNDEEPDASIIRSGGVEIDEASYAAKLDGRGLDLTFKEFEL
                     LKYLAQHPGRVFSRQQLLQEVWGYDYFGGTRTVDVHVRRLRAKLGPEHEALIGTVRNV
                     GYRFVLPTGETADDRHRGPTASSAAHSQSRR"
     gene            7531..8457
                     /gene="mshD"
                     /locus_tag="VH419_08390"
     CDS             7531..8457
                     /gene="mshD"
                     /locus_tag="VH419_08390"
                     /EC_number="2.3.1.189"
                     /inference="COORDINATES: protein motif:HMM:TIGR03448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035447 - mycothiol synthase activity
                     [Evidence IEA];
                     GO_process: GO:0010125 - mycothiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycothiol synthase"
                     /protein_id="HEY7043671.1"
                     /translation="MTASTDDALDLTVVSAVRADDAQAVLVLEDAATVADGVSPLDDQ
                     VRLDLRHPDDESGRVTHVLARRAGDLVGYAHARTTDDGPLSGHVVVAPDHRRHGTGTA
                     LVEKLLELAGDRPLRVWAHGDEAAANLLSERLGFSRVRDLWQMQRRLDLPIGSAVYPD
                     DVYVRTFEVGQDEAAWVAVNAAAFANHPEQGALTEADLRQRMAQPWFDPRGFFLAARG
                     AEILGFHWTKVHSGDPDHPAFGEVYAVGVSPTAQGLGLGKALTTTGLLHLRERGLRTV
                     ILYVEGDNEPAIRLYEKLGFTRSALDVMYEHG"
     gene            8843..11155
                     /locus_tag="VH419_08395"
     CDS             8843..11155
                     /locus_tag="VH419_08395"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007078824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HEY7043672.1"
                     /translation="MSSDGAGQSAAGQAAAAETAAAERAAAERADVTGSEIRTPALTA
                     STAGDPGEGPNGLVSRSGPSDSFDIEPPYEPEDDDLPDGRFLDRELSWLRFNARVLEL
                     ADDHDLPLLERTRFLAIFASNLDEFFMVRVAGLKRRLAAGVAVQAASGLMPREVLEAI
                     WRESRELMLRHGEVFRDDVMAALAGQGIALLRWSDLQVDEQKQMQELFTERIFPVLTP
                     LAVDPAHPFPYISGLSLNLAVTIRNPESGKEHFARVKVPPIFNRFVALGDQRFVPLED
                     VIAAHLDQLFPGMEVLQHHTFRVTRNEDLDVEEDDAENLLQALERELQRRRFGSPVRL
                     EVEESIDSHVLELLVSELDVSDSEVFHLPGPLDLTGLLSIADLDRPELKYPAFVPHTH
                     RHLAEVESASPVDTFAALGRRDVLVHHPYDSFATSVQRFIEQAAADPHVLAIKQTLYR
                     TSGDSPIIDALIDAAEAGKQVLVLVEIKARFDEQANIRWARKLEQAGCHVVYGLVGLK
                     THCKLALVVRDEGVGIRQYVHIGTGNYNPKTARYYEDLGLLTCDEGITADVASLFNNL
                     SGFGRQRDYQHLLVAPESVRSGLLQRVEREIDHCRAGRPARIRFKLNSIVDEAMIDGL
                     YRASQAGVPVQLWIRGICALRPGVPGLSENISVRSTLGRFLEHSRIFWFENGGESQAW
                     IGSADLMHRNLDRRVETVVQIPSRSHVDELGSLLDLGFDAGTASWWLGPDGEWTRHHN
                     DAEDMPLADLQSILIALHRRRREAAIVVRR"
     gene            11193..12080
                     /locus_tag="VH419_08400"
     CDS             11193..12080
                     /locus_tag="VH419_08400"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEY7043673.1"
                     /translation="MPPSPSRRSRAVRAAGCVVWRMGSKEPEILLVHRPRYDDWSFPK
                     GKLDRGETAHAAAVREVAEETSLRVRLGPRLPGTTYTVSGGQRKDVDWWCAKAPKHAD
                     ITGYRPNAEIDDVRWVTVSRARTRLTYSYDVELLNAFATAAYDSSALLVVRHAHARSR
                     RGWRGDDAERPLNAEGKEQAHRLVPLFAAYGIKRVVSSDAVRCVDTVLPYVNNSKAKL
                     RLEACLSQEEFDDGAMRKIVDTELSRSRRVALCSHRPVLPPMMRRLGIEPVTLEPAAM
                     LVLHRRSGQLLSVEHLPKP"
     gene            complement(12077..13075)
                     /locus_tag="VH419_08405"
     CDS             complement(12077..13075)
                     /locus_tag="VH419_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017936596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HEY7043674.1"
                     /translation="MELALVFSVVAIALAFDYTNGFHDAANAIATSISTRALTPRIAL
                     TMAAVMNFAGALLGQEVANTVSEVITPPSGDAGLVVVLGGLAGAISWNLITWYFGLPS
                     SSSHALIGGLVGAAIASNSGVHWSTIVDKVLIPMVISPVVGFVIAFLLMTAIIWVFRR
                     RHPARVHRGFRMAQTVSASAMALGHGLQDAQKTMGVIFLALLTTGHASPGDDLPLWVI
                     VSAAGAISLGTYSGGWRIMRTMGRRIIHLDPARAFSAESTAASILYVTAYVFKAPIST
                     THVITAGVMGAGATKRLSAVRWGVAKTIVAAWIMTFPAAALVAAGVYGVLELFLPV"
     gene            complement(13079..13696)
                     /locus_tag="VH419_08410"
     CDS             complement(13079..13696)
                     /locus_tag="VH419_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012918610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HEY7043675.1"
                     /translation="MPSLFRPTDTSFYDMFSESAKNLVIGAELLAEMLSTSADRSAIA
                     KRMREAEHEGDESTHSIVRKVNTTFVTPFDREDIYRLAAGLDDVMDFMEEAVDLVNLY
                     EIEELPSELAEQVEVLQRASELTADAMPRLRTMRDLEEYWIEINRLENDGDKSYRRIV
                     AKLFSGQYDALTVMKLKDVVDSLEAAIDAFERVANIVEQIAVKES"
     gene            complement(13888..14094)
                     /locus_tag="VH419_08415"
     CDS             complement(13888..14094)
                     /locus_tag="VH419_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017936607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043676.1"
                     /translation="MSQEPSQPFKGEAHVNDQLDVAVHDSEVLAEIEMMTNLIIATSE
                     SEGPLSQGKIDEILGVGADDRPPY"
     gene            complement(14395..14973)
                     /locus_tag="VH419_08420"
     CDS             complement(14395..14973)
                     /locus_tag="VH419_08420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043677.1"
                     /translation="MKKLLGGVLAGVVLAAGLNGGAASALASHRDNANDASADIDINK
                     VVVSRAHKRAVVVHVTFSDFGRKMNAIQYFFDTKKSHPGPEYGAMIYRGKDDDAIKRV
                     DIYRMRTFKLRSSHRIACLWRYHWYYNGRGPGYFHAKFPLSCFEGNGGKAIRVHVKTW
                     NFTRYRGTGVHRHGTFGFYDNMGAPRAWTTWT"
     gene            complement(15125..15301)
                     /locus_tag="VH419_08425"
     CDS             complement(15125..15301)
                     /locus_tag="VH419_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020385453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3099 domain-containing protein"
                     /protein_id="HEY7043678.1"
                     /translation="MERKPDRKVAYFVVMGTCIGLIMLAWFVVRFFSTPAAIAMSVVA
                     AVLPPIAAFVGNRS"
     gene            complement(15307..16182)
                     /locus_tag="VH419_08430"
     CDS             complement(15307..16182)
                     /locus_tag="VH419_08430"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HEY7043679.1"
                     /translation="MTSPLRFYVDGEIVPADQANVSVLDAGFQSGDAIWEGLRVYHGH
                     VFRLEQHLDRLEHSAQALRITLPGGRDAVRDALLRTLAANEMTDETHVRLMVTRGTRS
                     TSGMDPRNAPAAGTLVVVPEYKPVDPDPAPQRLRTASVRRPQPQVLDPSIHHANQLNS
                     ILARLEVLDDPDVDAALMLDVHGFVAEADTANIFCVVDGRLATPAPTACLHGITRGVV
                     LDLAATAGLEADERALTLFDLYRADEVFLTGTVCELVPVTMVDGRDIGTGKPGPVWRS
                     LLDRYRSLVDAETAD"
     gene            complement(16192..17649)
                     /locus_tag="VH419_08435"
     CDS             complement(16192..17649)
                     /locus_tag="VH419_08435"
                     /inference="COORDINATES: protein motif:HMM:NF019285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HEY7043680.1"
                     /translation="MSELRGLVGLAVLIGIAVVFSKNRSGIRWRTAAAALTVQVVFAL
                     LVLRWGPGREALDWVSDRVTQLIGYTNEGTSFVFGPLTGVGSEDETIFALQVLPVIIF
                     LGALIGLLFYLRVIQYVTHVIGGGIAVLLRVSKIEAMYASTVIFLGQSEAPLMIAPYL
                     RGLTTAQLFTVMTSGFAAAAGSTLVGYSLLGAPLPYLLAATVMNAPAGLLMAKLVWPD
                     GTEDTARRRTTPRGESAEEKAAAAVGATRVHEDDAVRRGRDRDADVPETSGAASSDRL
                     GDDERHDRDSDDADEHADAASEDFDVRSVRDEESANAIDALGRGALAGGKIAVTVGAL
                     LIAFIALIAMANSILSGIGGWFGYDDLTFQKVLGWAFAPLAWLLGVPWDEAAQAGSYM
                     GEKTVLNEFVAFSSFGPHVPDLSSTTVVIVTFALAGLRELRLDRDPDRNPVQSATGAS
                     DQRRAARPASAAGRQPGQPGQRRHRRSGRATLTAR"
     gene            17792..>18010
                     /locus_tag="VH419_08440"
     CDS             17792..>18010
                     /locus_tag="VH419_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043681.1"
                     /translation="MKRFFVLGAACLLLAGCAEASGQTASRPLPTDPTTLTLIVTTHQ
                     QPGAHGTFYPEGAVPQVRLVDADGNRIAT"
BASE COUNT         3063 a         6163 c         5914 g         2870 t
ORIGIN      
        1 cagccgggac agcgcgtgca catggccgag gtcgaacagc tcgaactcgg ggaccacctc
       61 gcgctcctgc gagagctggt acaggtcctt gatgaacggc cacgggttga ggaagacgtc
      121 gtcggcgaag ttcgtcgtcc ccatcgtcag cgagcacgag tcgggctctg cgtcgagcac
      181 cttcaaccgc tgctcgagcg ggtcgtggac cgaaccaccg gtggagagct gtacgacgag
      241 cgaggtggcc tcgcgaacgg cggcaaccgc ttcggtgagc cgggcgatct cgagcgtcgg
      301 ccggtgcgcg gtgtcgcgga tgtgcaggtg gatcatcgcc gccccggccg cctcacatcg
      361 aacggcggtc tcggcgagct cttcgatcgt ggtcgggagc gcgggtacgt cctccttggc
      421 cgactcggcg ccggtcggcg ccaccgtgat catcgtcgtc ggatgaggca ccaggggcgg
      481 cacggtcatg gcgtcatcgt gccagactcg gacgcgtcca ttggacgggc atcttcggct
      541 gtgacaggag gaggcgttgc gagccgtcgt acagcgcgtg tcgcaggcgt cggtctgcgt
      601 cgacggcacg gtgaccgggt cgttcgacga ggccgggctc gtcgtgctcg tgggggtgac
      661 ccacgacgac acccctgcac aggcggccgc cctggcgacg aagatctggg agatgcgcat
      721 catgcgcgac gagttgtcgg cggctgacct ggcggctccg gtactcgtcg tgagccaatt
      781 cacgttgtac gccgacacgc gcaagggtcg ccgcccctcc tggtcggccg cagcacccgg
      841 gtccgtgtcc gaacccctct acgaggagtt ctgtcgccag ctgggtgccc tgggcgcaac
      901 ggtccatacc ggcaccttcg gggcacagat gcaggttcgg atcgtcaacg acggcccggt
      961 gaccgtgatc gtcgacacgg cgacgcctgg tcgtcgccgt tgaggaaacc tgaagaggcc
     1021 gctgagaaga acctgaggaa gggcgggcga acctcttctc gatttcccgg tctcggcgtt
     1081 tgttggcaga cttgacccgg ccctcgaccc catcgaccca gcgagttgga tgaccgacac
     1141 cacccgatac cacgaagacg gcggctcccc ggagccgtcg tctggtgttg tcgaccccgc
     1201 acgccgctcg tcggccgcct caggtctgct ttccgtggtg atcggggcgc ctcgacagcg
     1261 ctcgcgcggt ggacgccaca agaggaagcg caagaccgtc atcggtcggc atccggtcgt
     1321 cctcgcgctc gccctcgtgc tcacgctggt cgcgggttct gccggggggt acctgtggtg
     1381 gctcaaccat cagctgggca acatccagcg ggtcgacgca gggatctccg agaacccggc
     1441 gatcgacggc catgagggcg gcaagccgct gaacatcctg ctgctcggcg cagacaacgg
     1501 caacgcgcag accgagtcgg tcgccgacga cctggcggac ggcaagtgga ctccgttcga
     1561 gcaccgcagc gacacgatga tggtcgtcca cattccggcg gaccggaagt ccgtccagct
     1621 cgtgtcgatc ccgcgcgaca cctgggtgcc gatcgacggg tacccgtaca ccgacggaca
     1681 cggcaagatc aactccgcgt tcgcctacgg cgggccctca ctcgccaggc agacggtgga
     1741 ggacctcacc gggatccaca tcgaccacct cgcgatcatc gactgggccg gcttcaagga
     1801 cctcacgtcc gcgctcggcg gtgttcgcgt ctacgttccc gacacgttct acgacgacaa
     1861 gcaggacatc acgtgggaga agggctggca tgacctcgag ggcaaggacg cgctctcgta
     1921 cgtgcggacc cgccacggac tggccaacgg cgacttcgac cgcatcaagc ggcagcagaa
     1981 cttcctgcgc gcgacgatgg gccagttgct ctccagcggt acgacgcaca acgtcgtcaa
     2041 gttcaccaag gtcgtcggcg ccatgacgaa gtacctcgtc gtcgacaaca cctgggacaa
     2101 cggagagatc aagagcctgg cgctgtccat gcgcaacctc cacagccacg acgtcgactt
     2161 cctgaccgca ccgttcggct cgtacgacag gagccccgac ggacagagca tcgtgcgcct
     2221 cgcaccgaag aagagcacgg cactgttcgc ggccgtcgag gacgacgacg tccagcacta
     2281 cctggaagag aacccgaagt ccgcactcgc cggcgcccac tcgatcaact gacctcttcg
     2341 gacaagccgg ccaagccctc accgggcggc aggtacgctg acgcacaggg gagccgcgca
     2401 ccggcagatg gatcgtcacg caacgagaac aagggcggac atggacgaga aacccgggga
     2461 cgaagcgccg gcagcgacgc cgccgtcgcc acccccgccg gacgactcag agcagcccga
     2521 cgcctcaaca cccgccggtg aaccacccgc gaagaagcgg cggtgggtga tgcggcacaa
     2581 ggcactcacc gcggttgccg tcgtgctcgt tctggttgcg ggctgttccg ggggctactt
     2641 gtggtgggcg gaccgccagc tgtccagcat cccccggatc gacatccaca tccacacacc
     2701 gcccgagaag aaccatcacg agtccaacaa ggacctcaac atcctgctcc tgggagccga
     2761 ccacggcgcg gtcgggcagt cggtcgccga ggatctggcc gacggcaagt ggacgccgtt
     2821 caagcacctg tcggacacga tcatcgtcct gcacatcccg gcagacagga agtccgtcca
     2881 gatggtgtcg atcccgcggg acacgtgggt gcacatcgac ggctacccgt acgggaacca
     2941 ccacgcgaag atcaacgccg cgttctccta cggtgggccc gaactggcgt acaagaccgt
     3001 ccaacagttc accggtctca ccatcgacca cgtcgcggtc atcgactggg tcggcttccg
     3061 cgagctcacc gaagcgctcg gtggcgtgcg gatctacatc ccggagacgt tcaccgacta
     3121 caagcaggac gtcacatggg agaagggctg gcacacgctc gaaggcagtg atgcgctgaa
     3181 gtacgtgcgg acccggcatg gcctggccaa cggcgacttc gaccggatgg cgcggcagca
     3241 gaacttcctc agggccatca tggcgaaaat gttgtcgtcc tcgaccacgc acaacccgtt
     3301 gcggttcagc aaggtcttgg cgtcgttgtc gtcgtacctg acgatcgaca gcacatggga
     3361 caacgacgag ctcaggtcgc tggcttggtc actgcgtaac ctgcacgccg gcgacgtgga
     3421 cttcttcacc gcgccgttcg gctcctacgc cacgatcgac gggcagtcgg tcgtccgact
     3481 cgacatgcca cagttccgct tcctgatgcg ctcgatcgac gacgaccatg tcgagaagta
     3541 cgtcgcgcgc taccccggga accacctggc cagccagacc aacatcaact gatctggagc
     3601 cggggtccgg acgtcggtcg tccgtagtcg tcttggagcc gttcgatgtc gtcctcaccg
     3661 aaatagccgc cggtctgcac ctcgacgaag atcaggtccg tctcgccgtc gttcgcgact
     3721 cgatgtgccg ctcccagcgg tacatcgacc gtgctcccca ccgtgacctc gtgcagtacg
     3781 ccgtccaccg tcgcaacgcc gtgtcccgtg accacgaacc agtgctccgc ccgccggctg
     3841 tggcgctggt agctcagccg ctgccgaggg cgcacacaga tccgtttgac ccggaaaccg
     3901 ggacccgcgt ccacgacctc gtagtagccc caggggcgct cgtcgcgctc gatggccacg
     3961 ccgtcctcgt tccactgtcg ccgcctcggg ctcgttgctg cccgttcccg gcgttcagtg
     4021 taggacccgc tgactcaccg gggtctcgac aagctcgacc gccgaagccg ctgatcgagc
     4081 ccgtcgagat caacgcccgc ggaaaccctc cgggtctcga caagctcgac cgccgcagcc
     4141 cgctcgaccg gcggagccct tcagacggca atgcgtacct cggccacgtc ccctcgcgcc
     4201 gcggtgaccc gctggtcgac cggctcgacc ttgggggcga gcgtgcgcaa cgtccaatcc
     4261 ccaggcgcgg cgaagaagcg gaactggccg gtcgcgctcg tcgggacctc ggccgtgaac
     4321 tcgcccagcc ggtccagcag ccgcacgtag gcgccgctca ccgggtgatc gtcatggacg
     4381 acgaccccct ggatgatcga ctccttggcg acgtcgacgc cctccaggct cggaccacct
     4441 gcggtcgctc cgcacactca cgcctccccg ggatcgtcac cgacggctac cgggacaccg
     4501 accagggagc cgtactccgt ccacgaaccg tcgtagttct tcacgttcgg ctcatcgagg
     4561 atctcgtgca ggacgaacca ggtgtgggca ctgcgctcac cgattcggca gtacgcgatg
     4621 gtgtccttcg cgccgaactc gacgccggct tcggagtaca acgtgcgcag ctcgtcgtcg
     4681 gatcggaacg ttccgtcgtc gttcgcggcc ttgctccacg ggatgttcac cgccgtgggc
     4741 acgtggccac ctcgctgcgc ctgttcctga gggaggtggg cgggtgccag tagccgaccg
     4801 gcgaactcgt cgggagaccg cacatcgacg aggttcttgg tgccgatcgc ggcgacgacc
     4861 tcgtcacgga aggcgcggat gtcgaggtcc gggtccttgg cggtgtacga cgtcggctcg
     4921 cgcgtcgcca cctcctcggt cagctcgagg gagtcgagct cccacttctt gcggccaccg
     4981 tcgagcagca gcgccttctc gtggccgtac agcttgaagt accagtaggc ataggcggcg
     5041 aaccagttgt tgttgccgcc gtacagcacg accgtgtcgt cgttggcgat ccctcgcgcg
     5101 gacatcagtt gctcgaactg ttccttgtcg acgaagtccc gtcgtaccgg gtcctgcagg
     5161 tctgccttcc agtcgatctt gagtgccccc ttgatgtggc ccttgtcgta ggccgtggtg
     5221 tcctcgtcga cctcgacgag aacgatgcgg tcctggccgt cgtcgccgag gcgactctgg
     5281 acccagtcgg cgtcgaccag tacggcgtcg cgactcattt cggatacctc cgtggttgtt
     5341 gtggccgcag aagcggagag gagaagtcgg tgcgcgtgag gtgagtggtg acgtgccagc
     5401 tcaacgggta cgacacagtg ccgctgcgca gcggcagtgg tcgacggcgc ggcgcctggt
     5461 caggtacgtc gatgccatgc ggcaaggcta cgcggcgcct cgaccgcact cttgaagtcg
     5521 accgcatgat gagaccagcg tctcacagtc agcagccttc cttcttcggt ggtcgagctc
     5581 gtcgagaccc tgcgacgtcc tcggctggcg gacggggccg cgccggcgcc agcatgagac
     5641 caacagccag gcccgaccac ggaaggacag gcatgaccgt ccagctgaac cacacgatcg
     5701 tgaactgccg cgacaagcgc gcggcagcag acttcttcgc tgacatcctc ggcgtcggcc
     5761 cggcgacgga gttcggtccc ttcatcgtgc tcgagctgtc gaacgacgtc tcgttggact
     5821 tcctcgacca agcaccggag gtccacgcac agcactacgc cttccttgtc gatgagacgg
     5881 agttcgacga gatactcggc cgcatccgcg atcgcgggct gccctactgg gccgatccgt
     5941 tccagcgtca accggaacgc atcaacacca acgatggtgg gcgcggggtc tactggcagg
     6001 acccgaacgg gcacatgttg gagatcatca cgcgtcccta cggaagcgag ggctgagcag
     6061 gtccttgccc gggtccgacg gcgaggcggc gctgacgccg tatcccgccc cctgcgctac
     6121 acgggcgagg ccggcgaggc tttgccgatg gttccatcgc acgcccacgg gtcacggttt
     6181 gcgatctccg catcgcaaac ccactgtgtc catcgagggt ttgcgatgtc tacacgccgc
     6241 ttgggctgta aacatcgcaa accgcaaccc gggcattggc gcggcagaca cacgtcgcgc
     6301 gagacgttct gtccgtggat gtcgaggtgg cactcggtgc acgacacgcc ggcagaaccg
     6361 aacgaaactt gccacctcgg cggggtcagc cgccggcgaa gggcgggagc acctcgatca
     6421 cgtcgcctgc ggtcacggga acatcggcga ggtgcgcacg gtgtacgggc tggtcaccga
     6481 gcacgagcga ggagaccgac aagacgtcgc ggaaccgcgc gtcgtcgtgc agggcgtgga
     6541 cggcggccag cgccgcctgg acggtctgta cgccctcgac ggtgtcctcg acccgcccgg
     6601 cggcagctct ggcagcagcc cagtagcgaa cggtgacgac cggagggccg ctgtcgggag
     6661 ccgtcccatc agcggttgtc gcgacgaccg ggtcaggcat gtccggtatt gtctcagcag
     6721 caatggggcc cgggcgaacc gccgcgggcc tcacctgctc gggggcacgc gggaggaggc
     6781 gcgcgtgagc aacctgctgt tgctgacgaa cgcactgcag ccgtcagccg agctgcttcc
     6841 agccctggcg ctgctcccgc accacgtgcg catcctgcct gcagagccca ccgtgctcgt
     6901 cgaggcgccg gactgcgatg cggtcctggt cgacggtcgc cacgacctcg cttcggtgcg
     6961 cagcctgtgc cgcctcatcc ggacgaccgg ggtcgacgtc cccgtcctcg tcatcgtcac
     7021 cgagggcggc ctcaccgtcg tggccgccga ctggggtgtc gatgacgtgc tgctctcgac
     7081 cgcaggtcct gccgaggtgg aagcccggct gcggttgagc gcaggccggt tggcgctggc
     7141 tcgcaacgac gaggagccgg atgcgtcgat catcagaagc ggaggcgtcg agatcgacga
     7201 ggcgtcgtac gccgcgaagc tggacggtcg aggactcgac ctcacgttca aggagttcga
     7261 gctgctgaag tacctggctc agcacccggg acgtgtcttc agccgccagc aactgctgca
     7321 ggaggtctgg gggtacgact acttcggcgg cacccggacg gtcgacgtcc acgtccggcg
     7381 gttacgcgcg aagctcggcc ccgagcacga ggcgctgatc ggaacggtgc gcaacgtcgg
     7441 gtaccggttc gtgctcccga cgggagagac agcggacgac cgccaccgcg ggccgacagc
     7501 gtcgagtgca gcgcacagcc agtcacgacg atgaccgcgt ccacagacga tgccctcgac
     7561 ctgaccgtgg tgtccgcagt gagggcggat gacgcgcagg ccgtcctggt cctcgaggac
     7621 gcggcgaccg tagccgacgg cgtctctcca ctcgacgacc aggtccggct cgatctgcgg
     7681 catccggacg acgagtcggg ccgggtgacc cacgtcctgg ctcgtcgagc aggcgacctc
     7741 gtgggctacg cgcacgccag gacgaccgac gacggacctc tcagcgggca cgtggtggtc
     7801 gcaccggacc atcggcgaca tggcaccggc acggcactgg tcgagaagct gctcgagctg
     7861 gccggggacc ggccgctgcg cgtgtgggca cacggtgacg aggccgcggc gaacctgctg
     7921 tccgagcggc tgggcttcag ccgggtgcgt gacctgtggc agatgcaacg gcggctcgac
     7981 ctgccgattg gctccgcggt gtacccggac gacgtctacg tacgcacgtt cgaggtcggc
     8041 caggacgaag ctgcttgggt ggccgtcaac gctgctgcct tcgcgaacca ccctgagcag
     8101 ggcgcgctca ccgaagccga cctgcggcaa cggatggcac aaccgtggtt cgacccgcgc
     8161 ggcttcttcc tcgcagcgcg cggcgcggag atcctcggct tccactggac gaaggtgcac
     8221 tcaggcgatc cggaccatcc cgcgttcggc gaggtgtacg ccgtcggcgt ctcgccgacg
     8281 gctcaggggc tcggactcgg caaggcgctg accaccacgg ggctgttgca cctgcgcgag
     8341 cgcggcctgc gcacggtcat cctgtacgtc gagggcgaca acgagccggc gatcaggctc
     8401 tacgagaagc tcggcttcac ccggagcgcg ctcgacgtga tgtacgagca cgggtgaacc
     8461 cgggcgaaac tcaggattcc tcaggtttct ttgcgcggag acggtcgcga tggttaggcg
     8521 tggtcgcccg gcttccgccg caatgacgtc gggcgaatcg gcaacgagaa gcgggaaccg
     8581 ttcgtgcccg ctgaggccgg tcggccagct ctccgtccct tgtggtcgat gggtgactgg
     8641 cggttttcgg acatctgccg gttatccgcc ccgatctacc ttggtcccga ggtagaccca
     8701 ccgctcccaa ggcagatctg cctggctaac cgtccggttg gatggctacc caggcaaacc
     8761 accaccgacg cggcccatag aacctcagga gaaccaagaa cttcctcagt cgccgttcac
     8821 cgagtagtga gacgatgacg ccatgtcgag cgacggagca ggccagtccg cagcaggcca
     8881 ggccgcagca gctgagaccg cggcggccga gcgcgcggcg gccgagcgcg cggacgtcac
     8941 gggctcggag atccgaacac cagcgctcac ggcgtccact gccggcgatc ccggtgaagg
     9001 ccccaacggt ttggtctcgc gaagtggccc gagcgactcc ttcgacatcg aacctccgta
     9061 cgagcccgag gacgatgacc tgccggacgg ccggttcctc gaccgcgagc tgtcgtggct
     9121 gcgcttcaac gcccgcgtgc tcgagctcgc cgacgaccac gacctcccac tcctggagcg
     9181 gacccggttc ctggcgatct tcgccagcaa cctcgacgag ttcttcatgg tgcgggtcgc
     9241 cggactgaag cgacggctgg cggccggcgt ggcggtgcag gccgctagcg gcctcatgcc
     9301 gcgtgaggtg ctggaggcga tctggcgcga gtcgcgagag ttgatgctgc ggcacggcga
     9361 ggtcttcagg gacgacgtga tggcggcgct cgccggccag gggatcgcgc tgctgcgctg
     9421 gagcgacctt caggtcgacg agcagaagca gatgcaggag ctcttcaccg agcgcatctt
     9481 cccggtcctg acaccgctcg ccgtcgaccc ggcccacccg ttcccctaca tctccggtct
     9541 ctcgctcaac ctcgccgtga cgatccgcaa cccggagtcc ggcaaggaac acttcgcgcg
     9601 tgtcaaggtg ccgccgatct tcaaccgctt cgtggcgctc ggtgaccagc ggttcgtccc
     9661 cctcgaagac gtcatcgccg cccacctgga ccagctcttc cctggtatgg aagtcctgca
     9721 gcaccacacg ttccgcgtga cccgcaacga ggacctcgac gtcgaggagg acgacgcgga
     9781 gaacctgctc caggccctcg agcgcgaact gcagcgacgg cggttcggat cgccggttcg
     9841 cttggaggtc gaggagtcga tcgactcgca cgtgctcgag ctgctggtct cggagctcga
     9901 cgtgtccgac tcggaggtct tccacctgcc cggtccactc gacctgaccg gcctcctcag
     9961 catcgccgac ctcgatcgtc ccgagctgaa gtacccggcg ttcgtcccgc acacgcaccg
    10021 ccacctcgcg gaggtcgagt ccgcaagtcc ggtggacacg ttcgccgctc ttgggcgtcg
    10081 cgatgtcctc gtccaccacc cgtacgactc gttcgcgacc agcgtgcagc ggttcatcga
    10141 gcaggcggca gcggatccgc acgtcctcgc catcaagcag acgttgtacc ggacgtcggg
    10201 cgactcgccg atcatcgacg ccttgatcga tgccgccgag gccggcaaac aggtcctcgt
    10261 gctcgtcgag atcaaggcac ggttcgacga gcaggccaac atccggtggg cccgcaagct
    10321 cgagcaggcc gggtgccacg tcgtctacgg tctcgtcggg ctgaagaccc actgcaagct
    10381 ggctctcgtc gtccgcgacg agggcgtggg catccggcag tacgtgcaca tcgggacggg
    10441 caactacaac ccgaagaccg ctcgctacta cgaggacctc ggcttgctca cgtgtgacga
    10501 gggcatcacg gccgacgtcg caagcctgtt caacaacctg tccgggttcg gtcggcagcg
    10561 cgactaccag cacctgctcg tggccccgga gtcggtgcgc tcgggcctgc tccagcgggt
    10621 tgaacgcgag atcgaccact gccgtgccgg gcgtccggcg cgcatcaggt tcaagctgaa
    10681 ctcgatcgtc gacgaggcga tgatcgacgg gctctaccgt gcatcccagg caggcgtacc
    10741 cgtgcagctc tggatccgtg gcatctgcgc gctgcgcccg ggtgtccccg gcctgtcgga
    10801 gaacatctcg gtgcgcagca cgctcgggcg cttcctcgag cacagccgga tcttctggtt
    10861 cgagaacggc ggcgagtcac aagcctggat cggttctgca gacctgatgc accgcaacct
    10921 cgacaggcga gtggagaccg tcgtccagat cccgagccga agccacgtgg acgagctggg
    10981 atctctgctc gacctcggct tcgacgctgg cacggcatcg tggtggctcg gccctgacgg
    11041 ggagtggacc cggcaccaca acgacgcaga ggacatgccg ctggccgacc tccagtcgat
    11101 actgatcgca ctccaccggc ggcgcaggga ggccgccatc gtcgtacgcc gctgaggttc
    11161 gataccggag cgaccgtgca acacttttcc ccgtgccacc ctcgccatcg cgtcgttcca
    11221 gagctgtacg tgccgccggg tgcgtcgtgt ggcgcatggg aagcaaggaa ccggagatcc
    11281 tgctcgtcca tcgacctcgg tatgacgact ggtccttccc caaggggaag ctcgaccgtg
    11341 gcgaaacggc gcatgcagcg gccgtccgag aggtcgccga ggagacaagc ctccgggtac
    11401 gcctcggacc ccgattgccg ggcaccacgt acaccgtcag cggcgggcag cgcaaagacg
    11461 tggactggtg gtgcgccaag gcgcccaagc atgccgacat caccggctac cggccgaacg
    11521 ccgagatcga cgacgtgcgt tgggtgaccg tgtcgcgggc acgaacccgg ctgacctact
    11581 cgtacgacgt cgagcttctc aacgcgttcg cgacggcggc atacgactcg tctgcgctcc
    11641 tggtcgtgcg tcatgcgcat gcccggagcc gccgcggctg gcgcggcgac gacgccgaac
    11701 ggcccctcaa cgccgaggga aaggaacagg cgcaccggct cgtgccactg ttcgcggcgt
    11761 acgggatcaa gcgagtggtc agcagcgacg cggtgcgctg cgtcgacacc gtcctgccgt
    11821 acgtcaacaa cagcaaggcg aagctgcggc tcgaggcgtg cctgtcccag gaggagttcg
    11881 acgacggcgc gatgcggaag atcgtcgaca ccgagctgtc ccgctcgcgc cgggtcgcgc
    11941 tgtgctccca ccgcccggtg ctaccgccga tgatgcgacg tcttgggatc gagccggtca
    12001 ccctggagcc ggcggcgatg ctcgtcctgc accgccgcag tgggcagctc ctctccgtcg
    12061 agcacctgcc caagccctag accggcagga acagctccag gacgccgtag acgccggcgg
    12121 ccacgagtgc ggcggcgggg aacgtcatga tccaggcggc cacgatggtc ttcgccacac
    12181 cccagcgcac tgcggacaac cgcttggtcg cgccggcacc catcacgccg gccgtgatga
    12241 cgtgcgtcgt ggagatcggc gccttgaaga cgtacgccgt gacgtacagg atcgaggccg
    12301 ccgtcgactc ggccgagaac gcccgggccg ggtcgagatg gatgatgcgc cgacccatcg
    12361 tgcgcatgat gcgccaaccg ccggagtagg tgccgagcga gatcgccccc gccgccgaga
    12421 cgatgaccca caacggaagg tcgtcaccgg gagaggcgtg tccggtcgtg agcagtgcga
    12481 ggaagatgac acccatcgtc ttctgggcgt cctgcagacc atggccgaga gccatcgccg
    12541 aggctgagac ggtttgcgcc atgcggaagc cgcggtggac ccgggcagga tgccggcggc
    12601 ggaacaccca gatgatcgcc gtcatcagca ggaaggcgat gacgaacccg accaccggcg
    12661 agatcaccat cgggatcagc accttgtcga cgatcgtgga ccagtgcacg ccgctgttcg
    12721 aggcgatcgc cgcgccgacg aggcctccga tcagtgcatg tgacgaggac gagggcagac
    12781 cgaagtacca cgtgatcagg ttccagctga tggcgcccgc cagaccgccg aggaccacca
    12841 cgagacccgc gtcgccgctc ggcggcgtga tgacctccga gacggtgttg gcgacctctt
    12901 ggccgagcag ggcgccggcg aagttcatga ccgcggccat cgtcagcgcg atgcgcggtg
    12961 tcagcgcccg ggtcgagatc gacgtcgcga tggcgttggc ggcgtcgtgg aacccgttcg
    13021 tgtagtcgaa ggcgagcgcg atggcgacga cggagaagac gagcgcgagc tccatccgtc
    13081 aggactcctt gaccgcgatc tgctccacga tgttggcgac cctctcgaac gcgtcgatcg
    13141 ccgcctcgag cgagtccacg acgtccttca acttcatcac ggtcagggcg tcgtactggc
    13201 cgctgaacag cttggccacg atccggcggt acgacttgtc accgtcgttc tccagccggt
    13261 tgatctcgat ccagtactcc tcgaggtcac gcatcgtacg cagccgtggc atggcgtccg
    13321 cagtcagctc cgacgcacgc tgcaggacct cgacctgctc ggcgagctcg ctcggcagct
    13381 cttcgatctc gtagagattc acgagatcga cggcctcctc catgaagtcc atgacgtcgt
    13441 ccagaccggc cgccagacga tagatgtcct cacggtcgaa cggcgtcacg aacgtggtgt
    13501 tgaccttccg gacgatgctg tgggtgctct cgtcgccctc gtgctccgcc tcgcgcatgc
    13561 gtttggcgat cgcggaccgg tccgcgctgg tcgagagcat ctcggcgagg agctccgctc
    13621 cgatcaccag gttcttcgcc gactcgctga acatgtcgta gaacgacgtg tcagttgggc
    13681 ggaaaagcga tggcacggca actcccgtca gctgcgaaca agcccacgac caggctggtg
    13741 acctcggcca tgctaagaag ccacggccat gctagggggt gattcgctca ttcgcgcaac
    13801 tcacccgctg cgatctgtgc cattcgcaga ttcaggcatg ccttgcgtcc gtcccccgtg
    13861 atcgaccggc tcgccggctg cggccgctca gtacggcggt cggtcgtcgg cgccgacacc
    13921 aaggatctcg tcgatcttgc cttgtgacag cggtccctcg gactccgagg tcgcgatgat
    13981 caggttcgtc atcatctcga tctcggccaa cacctcggag tcgtgcacgg cgacgtcgag
    14041 ctggtcgttc acatgcgcct cgcccttgaa cggctggctg ggttcctgac tcaccgtgcc
    14101 tccaccggag ccagcccgcg gctgaccacg ggctgtgcct ggtacgtcga gcctaaccgt
    14161 gacgcgagca caggtgcatc cgaacggtcg tgacacgccg ggtcgacacg gcgcagcggg
    14221 cccaccaccg cctccagcat gtcaaagagc ctttccacgt tcacgacccg ccaacctatc
    14281 gccctcccgg gtcgaccaca tctgcggtct ccccattgtg gacgacgctt cgcgctgccc
    14341 gaacggcgtg gtccgatgtg cgcgggagtg accggttcag cgcacccgac cgggtcaggt
    14401 ccaggtcgtc caggctctgg gtgctcccat gttgtcgtag aagccgaacg tgccgtgcct
    14461 gtgcacgccc gtaccgcgat agcgggtgaa gttccacgtc ttgacgtgca cgcgaatggc
    14521 cttgccgccg ttcccctcga aacagctcag cgggaacttc gcgtggaagt agcccgggcc
    14581 gcggccgttg tagtaccagt ggtagcgcca caggcacgcg atccggtggc tgctacgcag
    14641 cttgaaggtg cgcatccggt agatgtcgac acgcttgatg gcgtcgtcgt ccttgcctcg
    14701 gtagatcatg gcgccgtact ccgggccggg gtgagacttc ttcgtgtcga agaagtactg
    14761 gatggcgttc atcttccggc cgaagtccga gaaggtcacg tggaccacga ctgctcgctt
    14821 gtgggcgcgt gagacgacca ccttgttgat gtcgatgtca gcgctcgcgt cgttggcatt
    14881 gtctcgatgg gacgccagcg cgctggccgc gccgccattg agcccggcag ccagaacgac
    14941 gccggccagg acgcccccca ggagcttctt catgtcaggt ccttcccaca tgggtggtgc
    15001 ggattccgcg caagcactgt aaccacgggt ggggtcaccc ggaccggatt gtggcttgtt
    15061 cgcgacgtat ccgtgacacc gccatcaacc gttcggcgta ccgcaccatc ggccggtcgg
    15121 ccgctcagct tcggttgccg acgaacgccg cgatcggagg aagcacggcc gcgaccacgc
    15181 tcatcgcgat cgcggcaggg gtcgagaaga accggaccac gaaccaggcg agcatgatca
    15241 ggccgatgca ggtccccatc acgacgaagt aggcgacctt gcggtcaggc ttgcgctcca
    15301 cttcagtcag tcagccgttt ctgcatccac gagcgaccga taccggtcga gcagcgagcg
    15361 ccacaccggt ccgggtttgc cggtcccgat gtcccgaccg tcgaccatcg tgaccggtac
    15421 cagctcgcag accgtacccg tgaggaacac ctcgtcggcc cggtagaggt cgaacaaggt
    15481 gagggccctc tcgtccgcct cgagtccggc ggtcgcggcg aggtcgagca cgactccgcg
    15541 agtgatgccg tgcaggcaag ccgtcggtgc cggggtcgcc aaccggccgt cgaccacaca
    15601 gaagatgttg gccgtgtccg cctcagcgac gaagccgtgg acgtccagca tcaaggccgc
    15661 gtccacatcg gggtcgtcga gcacctccag ccgggcgagg atcgagttga gctggttggc
    15721 gtggtggatg ctcgggtcga gcacctgagg ctgcggacgg cgtacgctcg cggtccgcag
    15781 ccgctgcggc gccgggtcgg gatcgacggg tttgtactcc ggtacgacga cgagcgtccc
    15841 ggccgccgga gcgttgcgag gatccattcc cgacgtgctg cgagtacccc gcgtcaccat
    15901 gagccgcaca tgggtctcgt ccgtcatctc gttggccgcg agcgtgcgga gcagcgcgtc
    15961 gcgaacggcg tcacggccac cgggaagtgt gatccgcagc gcctgcgcgg agtgctcgag
    16021 ccggtcgagg tgctgttcga gccggaacac gtggccgtgg tagacccgca ggccctccca
    16081 gatcgcgtca ccggactgga atcccgcgtc gaggacggag acgttcgcct ggtctgccgg
    16141 gacgatctcg ccgtcgacgt agaaccggag cggtgaggtc acaccgagac gctaccgcgc
    16201 cgtcagtgtg gcgcgaccac tccggcgatg gcggcgttgg ccaggttggc caggctgccg
    16261 gccagcagcg ctcgcaggcc gagccgcgcg acgctggtcc gaagctccgg tcgcagactg
    16321 gacagggttc cgatctggat cgcgatcgag gcgaagttcg cgaaggcccg cgagcgcgaa
    16381 cgtgacgatg accaccgtcg tcgacgacag gtcggggaca tgtgggccga aggacgagaa
    16441 ggcgacgaac tcgttcagca ccgtcttctc gcccatgtac gaccccgcct gtgctgcctc
    16501 atcccagggg acgccgagga gccacgccaa cggtgcgaag gcccagccga gcaccttctg
    16561 gaacgtgagg tcgtcgtagc cgaaccatcc tccgatccct gagaggatcg agttcgccat
    16621 ggcgatcaac gcgatgaacg cgatcagcaa cgcgcccacc gtgacggcga tcttgcctcc
    16681 ggcgagagcg ccacgaccga gagcgtcgat cgcgttcgcc gactcctcgt cccgcaccga
    16741 ccgcacgtcg aagtcttcgc tcgccgcgtc agcgtgctcg tctgcgtcgt cgctgtcgcg
    16801 gtcgtgcctc tcgtcgtcac cgagccggtc cgaggacgct gcgccggacg tctctgggac
    16861 gtcggcgtca cggtcccggc cacggcggac cgcgtcgtcc tcgtgaacac gggtcgcgcc
    16921 gactgcggcc gctgccttct cctcggctga ctcccctcgc ggcgtcgtac gccgtcgggc
    16981 ggtgtcctcc gtgccatccg gccacaccag cttcgccatg agcagaccag ccggggcgtt
    17041 catgaccgtg gcggccaaca ggtagggaag cggagcgccc agcagggagt accccaccag
    17101 cgtcgaaccg gccgctgccg cgaaaccgct cgtcatcacc gtgaacaact gtgcagtcgt
    17161 caggccgcgc aggtagggcg cgatcatcag cggcgcctcg ctctgtccga ggaagatcac
    17221 cgtgctggcg tacatcgcct cgatcttgct gactctcagc aaaacggcga tccccccgcc
    17281 gatgacgtgc gtgacgtact ggatcacgcg caggtagaac agcagcccga tgagcgcacc
    17341 gaggaagatg atcaccggca ggacctgcag ggcgaagatc gtctcgtcct ccgacccgac
    17401 gccggtcaac ggaccgaaca cgaagctggt cccctcgttg gtgtacccga tcagctgagt
    17461 cacccggtcc gacacccagt ccaacgcctc gcgccccggt ccccacctca gcacgagcag
    17521 cgcgaacacc acttgcaccg tcagtgccgc cgcggcggtg cgccaccgga tgcctgaacg
    17581 gttcttggag aacacgacag cgataccgat caggacggcg agcccgacca gccctctcaa
    17641 ctcactcatg cagggcgtgt acccagatcg acgactcgtg cgacgctcgc ctgctgatca
    17701 ccgtttcgca gcggtctgcc gactcatccg tcgacgtctg gcggagccgg gaccgcggaa
    17761 ccgtcgtgcc ggtcggtttc gtcgaaggcc catgaaaagg ttcttcgtac tcggggcggc
    17821 gtgcctgttg ctcgccggat gcgcggaggc gtcgggacaa accgcatccc gcccgctacc
    17881 aaccgatccg accacgctca cgctcatcgt gacgacccac cagcaacccg gggcgcatgg
    17941 caccttctac cccgaagggg cggtcccgca ggtcaggctc gtcgacgctg acggaaaccg
    18001 gatcgccacc
//
LOCUS       DATBFZ010000272        12305 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_1822335, whole genome shotgun sequence.
ACCESSION   DATBFZ010000272 DATBFZ010000000
VERSION     DATBFZ010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 12305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12305
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1822335"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            238..933
                     /locus_tag="VH419_08445"
     CDS             238..933
                     /locus_tag="VH419_08445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043682.1"
                     /translation="MIESAAVFSLLAGACGAASTFPYLRDTLRRSTVPHRGSWLIWGV
                     IEVVAVEAQRADGARWSLVPLAIQAAGTCIVFALSVRFGSGGVSRMDLALIALAGAGV
                     VGWLAVDEPLIATTCVIAADFLAALMMLPKTWREPGSETMATYAFASLGGAATVGAVG
                     TLSVSLLIYPVYFTLLNAGLALVIGHRSRLLRHPRRALAETSEPLSNATDDMQGRQPA
                     LALQAGAVADASE"
     gene            1001..1288
                     /locus_tag="VH419_08450"
     CDS             1001..1288
                     /locus_tag="VH419_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008364189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HEY7043683.1"
                     /translation="MDFEFEGPVIEWRGPAPFYFVRIPMEESEDIKFAAKGVEYWGQV
                     PVAVRIGDVEFTTALFPKDGRYLIPLKDAVRGSAGIEVDHVLSVELSVRRP"
     gene            1386..1763
                     /locus_tag="VH419_08455"
     CDS             1386..1763
                     /locus_tag="VH419_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020579170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY7043684.1"
                     /translation="MKINRRVIVFDAGDIEAESRFWAGLLGGTVHRDDDWHSIVVDGE
                     WVMGVQRAPNHVAPDWPSGVQQQQVHLDLHVEDLESAGRLASSLGGRRLQAPRRPADN
                     PDGDEMFAVYASPAGHPFCFGVH"
     gene            complement(1791..2243)
                     /locus_tag="VH419_08460"
     CDS             complement(1791..2243)
                     /locus_tag="VH419_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012918584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEY7043685.1"
                     /translation="MSEHVAEIRWRRAGRQFAYETYARDHEWRFENGLVVSASANPAY
                     LGSASRVDPEEAFVAALSSCHMLTFLAIAARKRFVVDEYVDHAVGVLEKNADGRMAIT
                     RVTLHPEIGWSGDPPDAAALATMHRLSHEQCFIANSVTTDVTVAGIDT"
     gene            complement(2308..2970)
                     /locus_tag="VH419_08465"
     CDS             complement(2308..2970)
                     /locus_tag="VH419_08465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043686.1"
                     /translation="MSTVLRSSAVTAVGPLIEKFAPSAGRWLGCFGMAVGVAMVVLEP
                     LTSERTNVTVILLGVALICVSWVVMVRPRAAAHERGLLLQNLVRDAAIPWPRIERCTV
                     GQTLIVTTTEAESFHGLGVTRSARTQMREQYGTSSIMLGGMRATGRKLAPERGPSMAE
                     GEWQAGTYTSYVESRIAGLASKGERGGQDETYEPFMAWAPVPLLLLAVAVACVLAAII
                     EA"
     gene            3273..4799
                     /locus_tag="VH419_08470"
     CDS             3273..4799
                     /locus_tag="VH419_08470"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH and CHAD domain-containing protein"
                     /protein_id="HEY7043687.1"
                     /translation="MTTDHAEVEDKYDVDDETAVPAFVGVDGVASVEPQVEQDLEATY
                     FDTADLALTKAGITIRRRTGGQDAGWHAKLPAPEARHEVRVPLSRAVKTVPKEIRSVV
                     QGVVREQPLLPVAMIRTRRRASRLLDKKGGVLAEFADDLVTGQRLSPGTEPTTRTWRE
                     WEIELVDGDASVLAAVGAALRDSGATPSWAGSKLARTLDQRMPQPPRALVEPDRKAPA
                     AGVVQHRLLEQVAELVRWDPLVRRDVPDSVHKMRVASRRLSHALATFRPLLDRSVTEP
                     IRDELTWLAGYLGDARDAEVLRARLLTALGAEDRHVVPATALRRVDRELSARYRSAHQ
                     RLMQVMESERYFALLDRLHAVAATPPLTELATEQVRDVLPGRVLHDWKRLKKRVVAAG
                     ETDDPVDRASLLHEARKAAKRVRYAGEPLVSLYAGSAKKFVSLHEDIQTTLGEHHDSA
                     VARDELRRLAGQADREGTDAFIFGVLYVREEAVGAAAEEEFADVWRRVSKKRRRHWLH
                     "
     gene            complement(4814..6463)
                     /locus_tag="VH419_08475"
     CDS             complement(4814..6463)
                     /locus_tag="VH419_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017933898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEY7043688.1"
                     /translation="MATATARTRQLPGRTSLRREIGFVGLIWASEGSIIGSGWLFGAQ
                     KALGIAGPAAVISWAIGGAAILILALVHAELGGMYPVSGGTARFPHYAFGGAAGASFG
                     WFSWLQAATVAPIEVLAMITYAQHYSFGHGWLKTNGGSDVLTASGLAVAIVLMAVLTA
                     INFLSVRKLAHTNSAATWWKVGIPLLTIFVLAIASFHASNFTAADGFNPDGPKGILSA
                     VSTGGIIFALLGFEQADQLAGESSNPKRDIPRAVIGSVVIGVIIYILLQIVFLGALPA
                     DQIQGTWANAAFNDLTGPFAEVATLVSLGWLATILYLDAIISPGGTGLIYVTGSSRVS
                     YGLSRNGYIPTWFEVTNRRAVPWVGLITAFIVGCICFLPFPSWQSLVGLITSASVLMY
                     AGAPLSFGVFRKRLPNADRPWRLPGGLVWSPLAFIIANLLILWSGWTTDWKLGVAILI
                     GYVILAANRLFKLNPITPDLNWRAAQWLPVYLVGMGLIVFLSDFGPLTHPWFPLWWDM
                     AAVAVFSVVIYYWALSVALSTEHIEAMVHEVVVPEEAELAA"
     gene            6667..7047
                     /locus_tag="VH419_08480"
     CDS             6667..7047
                     /locus_tag="VH419_08480"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEY7043689.1"
                     /translation="MAIQRVNVDELGRLPAFSHASIVGKQVFVAGTIGAAPGSLDLVP
                     GGIGPETRQVLENIERVLNTCGCGLADVAIVHVYITDMDNFSAMNEAYVDVMGDDPPA
                     RITVGCAELAIGALVEMDCVAFIP"
     gene            7119..7676
                     /locus_tag="VH419_08485"
     CDS             7119..7676
                     /locus_tag="VH419_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012919195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEY7043690.1"
                     /translation="MADSVVDLIMADHREVERLFDRMKADPDQRPMLVPVVTALLVAH
                     SRAEEAEVYPVARDEAGISEEVAHSQEEHAEAEALLERLKSLDHNSGEFHSVLDELIE
                     AVSHHVEEEESSVLPGMRDHLSPDRLAELGEAFMVARAEHMGDRPGEATRDELLQQAQ
                     NAGIEGAASMTKDQLDKELRRRAEE"
     gene            7813..8574
                     /locus_tag="VH419_08490"
     CDS             7813..8574
                     /locus_tag="VH419_08490"
                     /inference="COORDINATES: protein motif:HMM:NF015799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF and ANTAR domain-containing protein"
                     /protein_id="HEY7043691.1"
                     /translation="MPSDPRVRKAMADLAKTLGTPTDADDALEALTRAARDTIPGADY
                     ASISLLRDDTLETLAATDPIIMLADRTQAELRQGPMFGGITDDDLYVSEDLANDPRWP
                     EYGPKAAALGLGAQMGVDLHHPAGGRAMLNLYAKRTWLFVDAFEIADLFASHASLVLG
                     LAQAVDHLSTALQSRKAIGQALGIVMERYTIDEDRAFDFLVRTSRNSNVKLRDIAVDI
                     VAGVNRRNRLDGGPPAPNTPALPEMAQLEKQESAG"
     gene            8787..9161
                     /locus_tag="VH419_08495"
     CDS             8787..9161
                     /locus_tag="VH419_08495"
                     /inference="COORDINATES: protein motif:HMM:NF013962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEY7043692.1"
                     /translation="MAVDLTIYLDDRPGELARLGEVLGAAGVNIDGFCVLTSGGGAAE
                     THVLVDDMDGAFSALETAGMRVASEQEVMVVEVEDRPGVLGEVTRRLGDADVNITLAY
                     LATRTRLVLAADELAAIKSALT"
     gene            complement(9259..9630)
                     /locus_tag="VH419_08500"
     CDS             complement(9259..9630)
                     /locus_tag="VH419_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020580136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY7043693.1"
                     /translation="MPVRLHHIVIDTHDLPRLARFWAEALHWELLSERDREIVVGPSP
                     DAPIGLCFMPVDETKTSKNRVHLDLTTTDDDRADEIDRLLALGASRADVGQTGEESWD
                     VLADPEGNEFCVVRPKQTLVD"
     gene            complement(9759..>12305)
                     /locus_tag="VH419_08505"
     CDS             complement(9759..>12305)
                     /locus_tag="VH419_08505"
                     /inference="COORDINATES: protein motif:HMM:NF014214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M36 family metallopeptidase"
                     /protein_id="HEY7043694.1"
                     /translation="ATALGLTQRDIAGLRLARNYVDIAGTHHLSYVQYAGGVPLFGNG
                     LQANVTRDGRLLSLGGSPVSGLHAASVGSAITNGRSAVSTARRDQGDAAKVGPADDAR
                     AVLFQTPTGTRRAWETITMSAEHPALTVLDSATGAVLFRQPLQSDATSERTSNDNVGL
                     NFRYFPKAKRGGIAHRVNFTKLGWLPSHAKFLHGNNAVTWSDVNDDDKQSRSEQVPPR
                     APHSWMYKLKPFHLRAHGFKQFCDNPYPCSWNPNKPFSWRTNRAQNATQVFYFVNTWH
                     DHLARAPIGFTEAAGNFQKVNASHKGKGGDPVMAQTDDGASVDHGLPDGDHVDNAFMD
                     TPPDGRSPRMAMFLQHLPFTSYPDGDEWAPTNVGDEADTVYHEYTHGLSNRLVVDASG
                     RSTLGDVQAGAMGEAWSDWYAMDYLVQQGLQKDRPASGDVVLFQYDGEGVSLDRTEPI
                     DCAVGATTPRCPGGATGHRGGYTYADYGKVGGAPEVHDDGEIWGQTLWDLRSRLGSRT
                     SESLVTRAMELSPANPSFLDERNAILLADMAVFGGRHQNAIWGVFAHRGMGYFAGAID
                     GDDTTPGADFHRPPPAGTPIGGLTGTVTDSETGDPIANATVALAWEGSPFATNPSAKT
                     DADGTYSIGPVPVGRYPKLAVLPPNGFDLAQQPVRVVPGTKTVDLAVRRDWAAKAGGA
                     TATFDGPDYGPGCNGDAAIDQTTAFGWSSTVDPGADGQPSPDTPKSITITLPQTVDVS
                     SVEVNPTANCGDGGSASTGDYEVSISQDGGATWSDPIQGTFVFANRGHLNPVQLPADA
                     TGVTDVRFTMLAPQVFTDMGTYDPNSCPGAFSGCDFQDVTEIEVFGSAAQ"
BASE COUNT         2058 a         4023 c         4252 g         1972 t
ORIGIN      
        1 ctgcggcttc cacactcgtg ccaaccgtag cctcgttcga cctctgtcgg cctattcgca
       61 ctgtcccgtg accggtgctg acccgagacc gcggaccaga gccctcgagg gtcagggcca
      121 cagagtggga aagccgtatc agcgcgcccc ttcgtgactc ctcactggtt tacctggggg
      181 gcaaccgatg ggacaggctg caggccgccg acgcgcgggc gggccggagg tgcggcgatg
      241 atcgaatctg ccgcagtgtt cagcctgctc gcgggagcgt gcggtgctgc cagcacgttt
      301 ccctacctgc gcgacacact tcgccgttcc actgttccgc accgcggttc ctggttgatc
      361 tggggcgtga tcgaggttgt cgcggtggag gctcaacgcg ccgacggcgc ccgatggagc
      421 ttggtgccgc tcgccatcca ggctgcgggg acgtgcatcg tgttcgcgct gtcggtgcgg
      481 ttcgggagcg gtggcgtgag ccgtatggat ctggcactga tcgccttggc gggcgcgggt
      541 gtcgtgggct ggctggcggt cgatgagccc ttgatcgcca ccacgtgtgt gatcgcggcc
      601 gacttcctgg cggcgctgat gatgctgccg aagacctggc gggagccggg ctcggagacc
      661 atggccacgt acgcgttcgc gtctctcgga ggtgcggcga ccgtaggggc ggtggggacg
      721 ctgtcggtct cgctgctcat ctaccccgtg tacttcaccc tgctcaacgc cggccttgcc
      781 ctggtgatcg gccaccgatc tcggctcctt cgccacccgc gccgcgccct cgccgagacg
      841 agcgagccgc tgtccaacgc cacggacgac atgcaaggca gacaaccagc ccttgcgctg
      901 caagcaggag cagtcgcgga tgccagcgaa tgagaccacg ccgccgctgg cgttcgggcg
      961 gcgttgtgcg tgacgcgcga tactcgcttg cgtgagcttc atggacttcg agttcgaggg
     1021 tccggtgatc gagtggcggg gccctgcgcc gttctacttc gtgcggatac cgatggaaga
     1081 gagcgaagac atcaagttcg ctgccaaggg agtggagtac tggggtcaag taccggtggc
     1141 ggtgcggatc ggtgatgtcg agttcacgac cgcgctcttc ccgaaggacg gccgctatct
     1201 catcccgttg aaagacgcgg tccgcggctc agcggggatc gaggtggatc acgtgttgtc
     1261 ggtggagttg agcgtccgac gtccgtgagc gcgccgactc acccttgctg ttcatccgga
     1321 cgtagaacgt ccggtttgag cgctaccgtc gaatcgtcct cgtcggaaca gcgacaggag
     1381 cagctgtgaa gatcaatcgg cgagtcatcg tgttcgacgc cggcgacatc gaagccgaga
     1441 gcaggttctg ggccggcctg ctgggcggca ccgtccaccg cgatgacgac tggcacagca
     1501 tcgtggtcga cggcgagtgg gtcatgggcg tgcaacgtgc cccgaaccat gtggcgccgg
     1561 attggccgtc cggtgtccag cagcagcagg tgcacctcga tctgcatgtg gaggacctgg
     1621 agtcggccgg tcggctggcc agttcactcg gaggtcgtcg acttcaggct ccgcggagac
     1681 cggccgacaa ccccgacggc gacgagatgt tcgcggtcta cgccagcccg gcgggccacc
     1741 cgttctgctt cggcgtccac tgacctccgg gctggcggcg ctgcgcctac tcaggtgtcg
     1801 atccctgcga cggtgacgtc ggtcgtgacg gagttggcga tgaagcactg ctcgtgtgac
     1861 aaccggtgca tcgtggcgag cgccgccgcg tccggtgggt cgccggacca tccgatctcg
     1921 ggatgcaggg tgacacgggt gatggccatg cgtccgtcgg cgttcttctc caggacgccg
     1981 acggcgtggt cgacgtactc atccacgacg aaccgcttcc gagccgcgat cgcgaggaac
     2041 gtcagcatgt gacacgacga cagcgccgcc acgaacgctt cctcggggtc gacccgcgac
     2101 gcactgccga ggtatgccgg attggccgaa gcggagacga cgaggccgtt ctcgaaccgc
     2161 cactcgtgat cgcgagcgta ggtctcgtac gcgaactggc ggccggctcg ccgccaccgg
     2221 atctccgcga cgtgctcaga catggtcagc cacggtcagg cattggtaag gcatcggtcc
     2281 aacatggtca gacatggtca gacatggtca ggcttcgatg atggccgcca gcacgcacgc
     2341 gaccgccacc gcgagcagca gcaacgggac cggagcccat gccatgaacg gctcgtacgt
     2401 ctcatcctgg ccgccccgct cacccttcga ggcgaggccg gcgatgcggg actcgacgta
     2461 cgacgtgtag gtgcccgcct gccactcccc ctcggccatc gacggacccc gttccggcgc
     2521 gagcttgcgt cccgtcgccc gcatcccgcc gagcatgatc gaactggtcc cgtactgctc
     2581 gcgcatctgc gtgcgcgccg accgcgtcac gccgagcccg tggaaggact cggcctccgt
     2641 cgtggtgacg atcagcgtct gtccgacggt gcagcgctcg atacgcggcc acggaatggc
     2701 ggcgtcccgg acgaggttct gcagcagcag tccgcgctcg tgcgcagcgg cgcgcggtcg
     2761 caccatgacg acccagctca cgcagatcag agcgaccccg agcaggatga cggtgacgtt
     2821 cgtccgttcg gacgtcaacg gctcgagcac caccatcgcg acgccgaccg ccatgccgaa
     2881 gcaccccagc caccgaccgg ccgacggcgc gaacttctcg atgagcgggc cgactgccgt
     2941 gaccgccgaa ctccgcagca cagtggacat gcctccaccc tagtgagcgc ggtacgccgt
     3001 cctggccgcc tgcccgacaa cgatgtggcg acgaaacgga caccgaccga cggcacgtag
     3061 cagttcgcga cagggttctc ggcgtaccga gggacactag tgtccggcgt cggaggttcc
     3121 ttcacaggtg cggcgagggc aggtggatgc atcgcaaggc ggaggaggga ggcgatgcgc
     3181 agcatcgtcg accgacgaca acgccgcgag gcgccgcctg gaccggcgca acgggaaaaa
     3241 gacttccgac gcgggacact agaaccgaac caatgacgac ggaccacgcc gaggtcgagg
     3301 acaagtacga cgtcgacgac gagaccgctg ttcccgcctt cgtcggcgtg gacggcgtgg
     3361 ccagtgtgga gccacaggtc gagcaggacc tcgaggccac gtacttcgac actgctgatc
     3421 tggcgctgac gaaggcgggg atcacgattc ggcgcaggac gggcggccag gacgcaggtt
     3481 ggcacgcgaa gctcccggca cccgaagcca gacacgaggt acgtgtgccg ctgagcagag
     3541 cggtgaagac ggtcccaaag gagatccgct cggtcgtcca aggcgttgtc cgtgaacagc
     3601 cgctcctgcc ggtcgccatg atccggaccc gtcggcgagc gtcgcggctg ctggacaaga
     3661 agggtggggt gctcgcggag ttcgccgacg acctcgtgac agggcaacga ctgtctcccg
     3721 gcactgagcc gacgacgagg acctggcggg agtgggagat cgagctggtg gacggcgacg
     3781 cttcggtcct tgccgcggtc ggtgcggcgt tgcgcgacag cggagcgacg ccctcatggg
     3841 ccgggtcgaa gttggcgcgg acactggacc agcgcatgcc gcagccgcct cgtgcgctcg
     3901 tcgaacccga ccggaaggcg cctgctgccg gcgtcgtgca gcaccgactg ctggagcagg
     3961 tggccgagct tgtccgctgg gatccgctcg tacgccggga cgtgcccgac tcggtgcaca
     4021 agatgcgggt cgccagccgt cggctgagcc acgcgctggc gacgttccga ccgttgctcg
     4081 accgcagcgt gaccgagccg atccgcgatg agctgacctg gttggccggc tatttgggag
     4141 atgctcgcga tgccgaggtg ctgcgtgcgc gactgttgac cgcgctcggc gcggaggatc
     4201 gacacgtggt cccggcgacg gccctgcgac gggtcgaccg ggagctgtcc gcccgttacc
     4261 ggagcgctca ccagcggctc atgcaggtga tggagtcgga gcggtacttc gcgttgctgg
     4321 accggctgca cgccgtggcg gcgacaccgc cgttgaccga gctggctacg gagcaggtgc
     4381 gcgatgtcct tccggggcgc gtgctgcacg actggaagcg cctgaagaaa cgcgtcgtcg
     4441 cagcgggcga gaccgacgat ccggttgatc gcgcgagcct cctgcacgag gctcgcaagg
     4501 ccgccaagcg ggtcaggtac gccggtgagc cactcgtgtc actgtatgcc ggcagcgcca
     4561 agaagttcgt cagcctgcac gaggacatcc agaccaccct cggtgagcac cacgacagcg
     4621 ccgtcgccag agacgagctc cgccggctcg ccggacaggc tgatcgcgag ggcaccgacg
     4681 cgttcatctt cggtgtgctg tacgtccgcg aagaagcagt cggggctgcg gccgaggagg
     4741 agttcgccga cgtgtggcga cgcgtctcga agaagcgtcg tcgccactgg ctccactgac
     4801 gccgacgtgc tggtcaggcg gcgagctcgg cctcctcagg tacgacgacc tcgtgcacca
     4861 tcgcctcgat gtgctcggtg ctcagcgcta cggacaacgc ccagtagtag atcaccacgc
     4921 tgaacaccgc gaccgccgcc atgtcccacc acagcgggaa ccacgggtgg gtgagtggac
     4981 cgaagtcgct caggaacacg atcagtccca tgccgacgag gtagacgggg agccactgcg
     5041 cggcccgcca gttgaggtcg ggagtgatcg ggttgagctt gaacagccgg ttggccgcga
     5101 ggatgacgta cccgatgagg atggcgacgc cgagcttcca gtccgtcgtc cagcccgacc
     5161 acaggatcag caggttggcg atgatgaacg ccagcggtga ccagaccagg ccgcctggaa
     5221 gccgccacgg ccggtcggcg ttgggcagcc gcttgcggaa gacgccgaac gagagtggcg
     5281 cgccggcgta catcagcacg ctcgcgctgg tgatgagccc caccagcgac tgccagctcg
     5341 ggaacggcag gaagcagatg catccgacga tgaacgcggt gatcaggcct acccacggca
     5401 ccgcccggcg gttcgtgacc tcgaaccagg tcgggatgta gccgttgcgg ctcagcccgt
     5461 aggagacccg cgaggatccg gtcacgtaga tcagtccagt tcctccgggc gagatgatcg
     5521 cgtcgaggta caggatcgtc gccaaccacc caaggctgac cagggtggcg acctcggcaa
     5581 acggaccggt caggtcgttg aacgccgcat tcgcccaggt gccctggatc tggtcggccg
     5641 gcagtgcccc gaggaacacg atctgcaaca ggatgtagat gatcacgccg atcaccaccg
     5701 aaccgatgac ggcgcggggg atgtcccgct tcgggttgct gctctccccg gccagttggt
     5761 cggcctgttc gaaaccgagc agggcgaaga tgatgccccc cgtcgagacg gcggacagga
     5821 tcccctttgg tccgtcaggg ttgaagccgt cggcagcggt gaagttgctg gcgtggaacg
     5881 aagcaatggc gagcacgaag atcgtcagga gcgggatgcc gaccttccac caggtcgccg
     5941 cactgttggt gtgcgccagc ttgcggacac tcaggaagtt gatcgccgtg aggacagcca
     6001 tcagcacgat cgcgacagcc agaccagacg cggtgagtac gtcggagccg ccgttcgtct
     6061 tcagccatcc gtgtccgaat gagtagtgct gcgcataggt gatcatcgcc agcacttcga
     6121 tgggcgcgac ggtggccgcc tgcagccagg agaaccaccc gaacgacgct ccggccgcgc
     6181 ccccgaatgc gtagtggggg aaccgcgcgg tgccgccaga caccgggtac atcccgccga
     6241 gctcggcgtg cacgagcgcg agaatgagga tggccgcgcc cccgatggcc cacgagatga
     6301 cggcggccgg gccggcgatc ccgagcgcct tctgcgcccc gaacagccac cccgaaccga
     6361 tgatcgatcc ttctgatgcc cagatgaggc cgacgaaccc gatctccctg cgcagcgacg
     6421 tgcgccccgg gagttgtcgg gtccgagcag ttgctgttgc catttccgac tccttcgccg
     6481 cagcgaggtc agaccctgaa ctctggctgg cgatttccgt ctggctggcg atttcagtct
     6541 tgctcgccgt tcgagcgtcg tcgaaaatcc ttcgctgctt ctcagggttc gccggtcggc
     6601 gccgggattc ggccgcgccc gcaccaggcc tgcggcaggt cttgccgagg cgggtacaac
     6661 gtgggcatgg ccatccaacg tgtcaacgtc gacgagctcg gtcggctgcc cgccttctcg
     6721 cacgcgagca tcgtgggcaa gcaggtcttc gtcgcaggaa cgatcggggc ggctcccggt
     6781 tcactcgacc tggtgccggg aggtatcggt ccggagaccc ggcaggtcct cgagaacatc
     6841 gaacgcgtcc tcaacacatg cggctgcggg ctggccgacg tcgcgatcgt gcacgtctac
     6901 atcaccgaca tggacaactt ctcggcgatg aacgaggcct acgtggacgt gatgggcgac
     6961 gatccacccg cccgcatcac ggtgggatgc gctgagctcg cgatcggcgc gctggtcgag
     7021 atggactgcg tggcgttcat cccgtagacg tcagtacgcg tagaactccg accacggggt
     7081 actcgggagc ggtctcgccg aaccagaggg aggatcctgt ggcagacagc gtcgtcgacc
     7141 tgatcatggc ggaccatcgt gaggtggaac gactcttcga ccggatgaag gccgatccgg
     7201 accagcggcc catgctggtc ccggtcgtga ccgcgctgct ggtcgcccac agcagagccg
     7261 aggaggccga ggtctacccg gtcgcccgcg acgaagccgg catcagcgag gaggtcgccc
     7321 acagccagga ggagcatgcc gaggccgagg cgctgctcga acggctcaag tccctcgacc
     7381 acaactccgg tgagttccac tccgtcctgg acgaactgat cgaggccgtc tcgcatcacg
     7441 tcgaggaaga ggagtcctcg gtcctgccgg ggatgcggga ccacctcagc cctgaccggc
     7501 tggcggagct gggtgaggcg ttcatggtcg cgcgcgccga acacatgggc gaccggcctg
     7561 gagaagccac cagggacgaa ctgctccagc aggcgcagaa cgccggcatc gaaggtgcag
     7621 cgtcgatgac gaaggaccag ctcgacaagg agctacgccg ccgcgccgag gagtgaaagc
     7681 tggccggcga cgtcggccga cctgcgtgct gtctgagttc ttactcgtcg gggatagcca
     7741 acctcccccc gctcatctag catgagagcg ccccaagcag tgtgggcacc ccgatgaggg
     7801 agacaggtag agatgccttc agacccacgc gtgcgcaagg cgatggcgga cctggcgaag
     7861 acgctgggca cgcccaccga cgccgacgat gcgctggaag cgctgacgcg ggcggcgcgc
     7921 gacacgatac cgggggctga ctacgcgagc atctcgctgc tccgcgacga cacgctcgag
     7981 accctggcgg cgaccgaccc gatcatcatg ctggcggacc gcacgcaggc cgagcttcga
     8041 cagggtccga tgttcggcgg catcaccgac gacgacctgt acgtctccga agacctcgcc
     8101 aacgatccac ggtggccgga gtacggtccg aaagcggcag cgctcggcct cggcgcccag
     8161 atgggcgtgg acctccacca cccggcaggc ggccgagcga tgctcaacct gtacgccaag
     8221 cggacctggt tgttcgtcga cgccttcgag attgctgacc tcttcgcctc gcatgcgtcg
     8281 ctggtgctcg gccttgcaca ggccgtcgat cacctcagca ccgcgcttca gagccgcaag
     8341 gcgatcggtc aggccctcgg aatcgtgatg gagcgataca cgatcgacga ggaccgggcc
     8401 ttcgacttcc tcgtgcgtac gtcgcgcaac agcaacgtca agctgcgcga catcgccgtc
     8461 gacatcgtcg cgggcgtcaa ccgtcgcaac cggttggacg gcggaccacc agcaccgaac
     8521 acccccgcgt tgccggagat ggcgcagctc gagaagcagg agtcggccgg atagcgacga
     8581 cgaccacaac gtcggaccgc ccacccaccg agcgtcggga ctgggcggtc cgacgtattt
     8641 cgacccggcc cgagcggtcc ctcgcatcac ggcggccgct ggagcagact gtcactcgag
     8701 cggtgacgat gccgtgacga tgcggtgacg atgccgtggt gatggcatgg cgagggcgtg
     8761 acgacgcgag tcgacaggag caaccgatgg cggtggacct caccatctac ctcgacgacc
     8821 ggccagggga gctggcaagg ctgggcgagg tgctcggcgc cgcgggcgtg aacatcgacg
     8881 ggttctgcgt gctcaccagt ggtggcggag cggcggagac gcatgtgctg gttgacgaca
     8941 tggacggtgc cttcagcgcg ttggagaccg ccgggatgag ggtggcctcc gagcaggagg
     9001 tcatggtggt cgaggtcgag gatcggcctg gggtcctggg tgaggtgacg cgccggctcg
     9061 gcgatgcgga cgtcaacatc accttggcgt atctcgccac ccggacccga ctcgtgctcg
     9121 cggccgacga actcgccgcc atcaagtccg ccctcacctg acccttccgc cgcttcggct
     9181 ttccgcgaat tgcgggaaaa gggctggcga caccggcgat gaaacctgat tcttcccgca
     9241 tttcgcgggg gtggtgcgtc agtcgacgag ggtctgcttc ggccgtacga cgcagaactc
     9301 gttcccttcc gggtcggcga gcacgtccca ggattcttcg cccgtctgac caacgtcagc
     9361 ccgactcgcg ccgagtgcca gcaggcggtc gatctcgtcg gcgcggtcgt catcggtcgt
     9421 ggtcaggtcg aggtgcaccc ggttcttgct ggtcttcgtc tcgtcgaccg gcatgaagca
     9481 caggccgatc ggcgcatccg gactcggtcc cacgacgatc tcgcggtcgc gctcggacag
     9541 gagctcccag tgcagcgcct cggcccagaa acgtgccagc cgcggcaggt cgtgggtgtc
     9601 gatgacgatg tgatgcaggc gcacaggcat gcggctcatt ctcccgccgc cttccgggtc
     9661 accgcgaatt gcgggaaaag ggctgccgac acgccaatgt cggggaccac tttcccgcaa
     9721 ttcgcggaag caacagggct gagtcagttg caggtttcct actgcgccgc tgacccgaag
     9781 acctcgatct cggtgacgtc ctggaagtca cagccggaga acgcccccgg acagctgttc
     9841 ggatcatagg tacccatgtc ggtgaacacc tgcggcgcca gcatggtgaa ccgcacgtcc
     9901 gtcacgccgg tcgcgtcggc ggggagctgc accgggttca gatgcccacg gttggcgaaa
     9961 acgaaggtgc cctggatcgg atcggaccac gtggctccgc catcctgcga gatcgacacc
    10021 tcgtagtcac cggtggaggc actgccgccg tcaccgcagt tggcggtcgg gttgacctcc
    10081 accgacgaga cgtcgaccgt ctggggcagc gtgatcgtga tcgacttcgg cgtgtccggt
    10141 gacggctggc cgtcggcgcc ggggtcaacg gtgctgctcc aaccgaacgc cgtggtctgg
    10201 tcgatggcgg cgtcgccgtt acagccgggc ccgtagtccg ggccgtcgaa cgtcgccgtc
    10261 gcaccccccg ccttggcggc ccagtcacgt cgcacggcaa ggtcgaccgt cttcgttccc
    10321 gggaccacgc gcaccggctg ctgcgccaga tcgaagccgt tcggcggaag caccgccagc
    10381 ttcgggtacc gtccgaccgg cacgggcccg atcgagtagg tgccgtcggc gtcggtcttg
    10441 gcggacgggt tggtcgcgaa cggcgaaccc tcccaggcca aggcgacggt ggcgttcgcg
    10501 atggggtcac ctgtctcgga gtccgtcacg gttcccgtca agccgccgat cggcgtacct
    10561 gccggtggcg gccggtggaa gtcggcacct ggcgtggtgt cgtcgccgtc gatggcaccc
    10621 gcgaagtagc ccatgccgcg gtgggcgaac actccccaga tcgcgttctg gtgcctgccg
    10681 ccgaacaccg ccatgtcggc aagcaggatc gcgttgcgct cgtcgaggaa cgacgggttg
    10741 gcgggcgaca gttccatcgc acgggtcacg agcgactcgc tggtcctgga accgagccga
    10801 ctgcgcagat cccagagtgt ctggccccag atctcgccgt cgtcgtgcac ctccggagca
    10861 ccaccgacct tgccgtagtc ggcgtacgtg tagccgccgc ggtgcccggt cgctccgccg
    10921 ggacaccggg gcgtggtcgc gcccaccgca cagtcgatcg gctcggtgcg gtcgagtgac
    10981 acaccttcac cgtcgtactg gaacagcacg acgtcaccgg atgccggccg gtccttctgg
    11041 agcccttgct ggaccagata gtccatcgcg taccagtcgc tccacgcctc gcccatcgcg
    11101 ccggcctgga cgtcgcccaa cgtggacctg ccgctggcgt cgacgacgag ccggttggac
    11161 aggccgtggg tgtactcgtg gtagaccgtg tcggcctcgt caccgacgtt cgtcggagcc
    11221 cactcgtcgc cgtcgggata gctggtgaac ggaaggtgct gcaagaacat cgccatgcgt
    11281 gggctgcgac catctggtgg agtgtccatg aaggcgttgt cgacatggtc gccatccggc
    11341 agaccgtggt cgacggaggc gccgtcgtcg gtctgcgcca tgacggggtc gccgcccttg
    11401 cccttgtgcg aggcgttgac cttctggaag ttgccagcgg cctcggtgaa accgatcggc
    11461 gcacgcgcga ggtggtcgtg ccaggtgttg acgaagtaga acacctgggt ggcgttctgc
    11521 gcgcggttgg tgcgccacga gaacggcttg ttcgggttcc acgagcacgg gtacgggttg
    11581 tcgcagaact gcttgaaacc gtgcgccctg aggtggaacg gcttgagctt gtacatccac
    11641 gagtgcggag cccgcggcgg gacctgctcg ctccgggact gcttgtcgtc gtcgttgacg
    11701 tcggaccagg tcacggcgtt gttgccgtgc aggaacttcg cgtggctggg cagccagccg
    11761 agcttggtga agttgacccg gtgagcgatt ccgcctcgct tcgccttcgg gaagtagcgg
    11821 aagttgaggc cgacgttgtc gttgctggtc cgctcgctgg tcgcgtcgct ctgcaagggc
    11881 tgacggaaca gcaccgcacc ggtcgccgag tcgagcacgg tgagtgcagg gtgctcggcc
    11941 gacatcgtga tcgtctccca ggcacgacgg gtcccggtcg gggtctggaa cagtacggcg
    12001 cgggcgtcat cggctggacc gacctttgcg gcgtcaccct ggtcacgacg agccgtcgac
    12061 acggccgatc gcccgttggt gatggcagac ccgactgatg cagcatgcag tccgctgacc
    12121 ggtgatccgc cgaggctgag gagccgacca tcccgcgtga cgttggcctg cagcccgttc
    12181 ccgaacagcg gtacgccgcc ggcgtactgc acgtaggaca gatggtgggt gccggcgatg
    12241 tcgacatagt tgcgcgccaa cctgagaccg gcgatgtcgc gctgcgtcaa cccgagcgcg
    12301 gtcgc
//
LOCUS       DATBFZ010000273         3393 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_1171388, whole genome shotgun sequence.
ACCESSION   DATBFZ010000273 DATBFZ010000000
VERSION     DATBFZ010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 3393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3393
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1171388"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1637
                     /locus_tag="VH419_08510"
     CDS             <1..1637
                     /locus_tag="VH419_08510"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEY7043695.1"
                     /translation="GRLLVLIGYAAGAVVGLLSVLHSEITVSASSLDKAPHAASPLPE
                     SLTSLVDPLPVMDSAMFLVLPVALVALLVRYSGADETERKQIRWPLASIGLLAVMILV
                     AETGLQPSDWGQNSAWASIIWGLMLTTVPISLLLGIVVYHALDIDVILRRSVVYGLLW
                     LAIAATYAAVAAMLGVAVSQRISVQVAILVTIVAAMVFQPARVRLERLADRLVFGERL
                     EGYELMRRVGSTLERSGRPEEVAGWLAATTRTGMHATWARVALVTAVGDTARVVAADG
                     IDAAATEAPALSVPLSDGGRLECGPRVDGGYRASDVDLLTTLARQASLAVTNAQLSVD
                     LARSRVRIVNAETAERRRIERDIHDGVQQQLVALMAKVSVARAQLDGEPARASGTLDA
                     MQQELRDALSDLRDLASGIHPPVLTDSGLLSAVESRAARLPIGVSIVWRADGVDRRFD
                     PQIEAAAYFSISEALTNVLKHSGADSAEVQVVCVDGTLDIAVCDRGVGFDTTAQRSRG
                     LQGMRDRVEALGGRLHVVSGVGDGTTVQVRLPAVAP"
     gene            1634..2326
                     /locus_tag="VH419_08515"
     CDS             1634..2326
                     /locus_tag="VH419_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009083503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY7043696.1"
                     /translation="MTEAAVLRVVLAEDNYLVREGTRQVLEAGDGVSVVATVGDAAAL
                     LEAVEDHRPDAVVTDIRMPPSHHMEGIEAAHLIRERWPQIGVVVLSQYADEAYAFALL
                     ERGTAGLAYLLKERVLDRADLVRALRETADGRSVLDPLVVDALVSRRARQERSKLSDL
                     TDRETAVLSLMAEGHTNAGIGRELHLSASAVEKHINAIFTKLGLSPETEVHRRVAAVV
                     TFLQHSGHTSAP"
BASE COUNT          528 a         1115 c         1165 g          585 t
ORIGIN      
        1 ggggacgcct gctcgtcctc atcgggtacg ccgccggagc agtcgtcggg ctcctcagcg
       61 tcctgcacag cgagatcacc gtctccgcct cgagcctgga caaggctcca catgccgcgt
      121 caccgctgcc cgagtccctg acctcgctcg tcgatccgct gccggtgatg gacagcgcca
      181 tgttcctggt gctccctgtg gcgttggttg cgctgctggt gcggtactcc ggtgccgacg
      241 agaccgagcg gaagcagatc cgttggccgc tcgcctccat cggcctcctc gccgtgatga
      301 tcctggtcgc cgagaccggg ctgcaaccgt ctgactgggg gcagaactcc gcatgggcgt
      361 cgatcatctg gggtctgatg ctgacgaccg tcccgatcag cctgctgctc ggaatcgtcg
      421 tctaccacgc gctcgacatc gacgtgatcc tgcggcgttc cgtcgtgtac ggcctgctgt
      481 ggttggcgat cgcggcgacg tacgcagcgg tggcagcgat gctcggcgtg gcggtgagcc
      541 aacgcatctc tgtccaggtc gcgatcctgg tgacgatcgt cgctgcgatg gtgttccaac
      601 cggctcgcgt ccggctcgag cgtctcgccg accgcctcgt cttcggcgaa cgtctcgagg
      661 gttatgagtt gatgcgacgg gtggggtcga cactggagcg gtcgggacgg cccgaggagg
      721 tggccggttg gctcgccgcg acaactcgca ccgggatgca tgccacgtgg gccagggttg
      781 ccctggtcac agcagtgggt gatacggcac gcgttgtcgc ggccgacggc atcgacgccg
      841 cggccacgga agcaccggcg ctttcggttc cgttgtccga cggtggtcgg ctcgagtgcg
      901 gaccgcgcgt cgacggtggc tacagagcat ccgacgtcga cctcctgacc acgctcgcca
      961 gacaggcgtc gctggctgtc accaacgcac agctgtcggt cgacctggcc cgctcacgtg
     1021 tccggatcgt caacgccgag acggcggagc gtcggcgcat cgagcgggac atccacgacg
     1081 gcgtgcagca acagctcgtc gcgctgatgg cgaaggtctc ggtcgctcgc gcgcaactcg
     1141 acggcgaacc cgcgcgtgcc agtggcacgc tcgacgccat gcagcaggag cttcgtgatg
     1201 ccctctcgga cctgcgtgac ctggcgagcg ggatccaccc accggtactc accgactccg
     1261 gcctgttgtc cgcggtggag tcccgcgccg cacggctgcc catcggggtg tccatcgtct
     1321 ggcgagccga tggggtcgat cgacggttcg acccacagat cgaggcagcg gcgtacttct
     1381 cgatctcgga ggcgctcacg aacgtgctca agcactccgg cgctgacagc gccgaggtcc
     1441 aggtcgtgtg cgtcgacggc acgttggaca tcgccgtgtg cgaccgtggc gtcgggttcg
     1501 acaccacggc tcagcgcagt cgcggcttgc aaggtatgcg cgaccgcgtc gaggcgctcg
     1561 gcggacggtt gcacgtcgtc agcggggtcg gcgacggtac gacggtgcag gtccggctac
     1621 cggcggtggc accatgaccg aggccgcggt gctccgcgtc gttctcgcag aggacaacta
     1681 tctcgtacga gaaggcaccc gccaggtcct ggaagcaggc gacggggtgt ccgttgtcgc
     1741 cacggtcggt gatgccgcgg ccctgctgga ggccgtggag gaccatcggc cggacgcggt
     1801 ggtcaccgac atccggatgc cgccgagcca ccacatggag ggcatcgagg ccgcgcacct
     1861 gatccgtgag cgatggccgc agatcggcgt cgtcgtgctt tcgcagtacg ccgacgaggc
     1921 ctacgccttc gccctcctcg agcgcggcac ggccggtctg gcctacctgt tgaaagagcg
     1981 tgtgctcgac cgcgccgacc tcgtgcgggc cctccgcgag acggcggacg gcaggtccgt
     2041 gctcgatccg ctcgtcgtcg acgcactcgt ctcccgtcgc gcccggcagg agcgctcgaa
     2101 gctgtccgac ctcaccgacc gcgagacggc cgtgctgtca ctgatggccg agggacacac
     2161 caacgcgggc atcggacgcg agctccatct ctctgcgtcg gcggtggaga agcacatcaa
     2221 cgcgatcttc accaagctcg gactcagtcc ggagaccgag gtgcaccgtc gagtggccgc
     2281 cgtcgtcacc ttcctccaac acagcggtca cacgtcggca ccgtgagcgt gccttggtgt
     2341 ctcgttgggc aacccttgtg gtttgccttg gtggccatgc aaacggattg gtagtcagca
     2401 gaaatctcgg ttcgccccag gttctgtggg tggagatggt cgcgatgggg tgggcgtggt
     2461 cgtccggcgg actgtcatgg tgggctgagg tggatcggcg gcgaagagcg ggagacgttc
     2521 ctgccagctg agctcgtcgg ccggcgttcc gtttgactgg tgtgtgactg gcggattgcg
     2581 gacatccgga gctgaccggc cctaagtttg cgatgtcaag acggcaaacc ctctgtcccc
     2641 atcgaaggtt tgcgatgttt acacgccttt tgggctgtaa acatcgcaaa cctcaagccc
     2701 ggactgtccg aatagtggcg taggtggctt tccggaggcc gggtgggccg cgccggtgca
     2761 cgggcaggac ggcagtcctg cccgtatcgc ctgcccggaa ccggtgcatc gagcctcacg
     2821 agtcaaggga cgccgaaggc ggtcgcgtca gcgatcgcga cagcgaccct tgacgcgtga
     2881 ggaatgcacc ggtcgcaggc gcggcccaca aacctgacgg tcgagcctgc caagacccct
     2941 cgatctcgac cagcttgatc agcggggttg acgcgtgagg aatgcaccgg tagcgggcgc
     3001 ggccacaatc ctgccggtcg agcctgtcga gacccctcga tctcgaccag ctcgatcagc
     3061 ggggttgacg catgaggaat gcaccggtag caggcgcggc ccacaggcag gcggccatca
     3121 cgcgcacaaa cccgagcagc cgcacatctc tggcaccact tccccgcggt ggtgccagag
     3181 ccggcgtgga cgttgcccga ccgcctacgg ccgatgcctc agaagccgct ggtgatcaag
     3241 gcggtcatcc tcggcgtacc gataccggtt gcaaggtcgt agccacgcct cgcagagaac
     3301 gcgccgttgt tcttggccac gtgtccccct ccggtcacgt cgtggaagaa gcgacgtgga
     3361 gcggcattgt aggcgagata caacaacggg ttc
//
LOCUS       DATBFZ010000274         5660 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_842544, whole genome shotgun sequence.
ACCESSION   DATBFZ010000274 DATBFZ010000000
VERSION     DATBFZ010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 5660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5660
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_842544"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="VH419_08520"
     CDS             <1..265
                     /locus_tag="VH419_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017934900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HEY7043697.1"
                     /translation="AHPTAESVLREVVEKVVASHDVRRVAAVHRVGELAIGDVAVAVA
                     VSCDHRGDAFTACRALIDDLKATVPIWKHQWFRDGSDEWVGTP"
     gene            365..655
                     /locus_tag="VH419_08525"
     CDS             365..655
                     /locus_tag="VH419_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017934901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043698.1"
                     /translation="MTAGRFTGGAMAVLWWLVPPLVTTCLAMVWVGWLGRERDETRRD
                     DSEAALQRMQKALSRPAPTKGKPVMSTPIEPTHGVAIRGAAHRPVAPTKSAR"
     gene            669..1724
                     /locus_tag="VH419_08530"
     CDS             669..1724
                     /locus_tag="VH419_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020575838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEY7043699.1"
                     /translation="MTRRTITLLGAGVVLGALLLVAFFAPMPYVVMSPGLTENTLGSF
                     DGKQVITIDGHKTYPTSGRLDLTTVSVTSPDYSPRLSEILQAWWDKDEIVIPRDVAYP
                     PSQSVEQVQQQNRTDMLDSQQSAIAAGLGEAGIPSVYVTVSDVEKDAPANGVLKTGDL
                     ITSVDGTTIHSVDAAISGISSVEPGSDVTLGIKRNDKDEQVTITTEASEDDPSKARIG
                     VGLGETIDPPFTVKINLGQNIGGPSAGTMFALAIYDMITPGPLTGGRHVAGTGTITPE
                     GRVGVIGGVQQKIAGAYREGATVFLVPAGDCAEAAKSPLADDIQLVKVETLDQAVNAL
                     EGIDSGDTSAVTPCTTG"
     gene            1745..2320
                     /locus_tag="VH419_08535"
     CDS             1745..2320
                     /locus_tag="VH419_08535"
                     /inference="COORDINATES: protein motif:HMM:NF040618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPA1309 family protein"
                     /protein_id="HEY7043700.1"
                     /translation="MSRRTARQDGGAAYSGEVSERMLGLRVAVVEVERHTSEAGWDQA
                     ARLFALVRTVDLAAAEPELAAELGINDGTADLFTPIEQELDEHTGSLEELLGRITWPE
                     TVDGALAVVERLVLPPDAEEAVPDDPGKAAEFAAGHHARQDVRMAAGVLRSGEAHCVL
                     RVRSHDSDDALLHGPDVVPGLVSALRETLEP"
     gene            2393..5386
                     /locus_tag="VH419_08540"
     CDS             2393..5386
                     /locus_tag="VH419_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HEY7043701.1"
                     /translation="MFEEEAQPERPPRRRQPSQRPRALLPTLGIVVALVVLFSVFVEV
                     WTGRLWFQALGYQGVFTKVLWTRTALFFIFGLLLAGIVVGNALLAFRVRPILFADGYR
                     NPTVERYQDTVDPIRHWVLIGLGVIMFLFGGASASGEWKTYLLWQHRVPFGQNDVYFG
                     KDIGFFVFSYPWYRFLISFGFTALVIAMIVTAATHYLYGGIRLGAKREKFSHAAQVQL
                     SVLFGLFMLLKAVSYWLDRYGLAISSGHLFTGVSYADANAVLPAKNILMIIALICALL
                     FFGNVIRPGWMLPVLGFGLLVLSAILIGGIWPAIVQRFQVKPSEADKEARYIELNINA
                     TRDAYGLNEIKPVSYPGVTTDTPLQLQKLASELPGVRLIDPKLVAPAFEQLQQQRGFY
                     KMPDVLDVDRYKFTGDAQPQDVVLAARELSLNGLRDDQRNWTNDHTVYTHGYGVVAAY
                     GDERGSAGEPVWAQQGLPSEGQLGDFRQEVYYGESEPSYSIVGAPEGTPPVELNIPAV
                     GSGGTDTDQNSTYEGDGGVPLGSTLNQVLYAAKFWDSSILLSGRVNPESKIIYDRSPR
                     TMVEKVAPWLTVDSDTYPAVVDGRLKWILDGYTTTSDYPMSERVDLSETTSDSLTAED
                     AVAAQKSDDINYIRNSVKATVDAYDGTVTLYQWDENDPVLQAWMGAFPGVVQPKSEIS
                     DDLMAHLRYPEDLFKVQRELLSTYHVTNPKTFYSGSENWRVPEDPNADNTAAKQPPYF
                     LTVKLPEDVTSETHQESAPEFSLTSVYEPNSRQNLASFVAVNADATSPDYGKFTVLEL
                     PSDNAVSGPSQVANAMSNDPKVRNELLKFTQVGTKVLYGNLLTLPVGDELLYAEPVYT
                     LRGGTGTGTYPILQFVVVSIGDDVGIGDSFEAAFDSALNLEEATTPPPTENPPPPPSQ
                     GGNGKPGQQTTNQQITALLGEAQDFFTRAQAALNRGDLGEYQRLNDKGYAKLAQAIAL
                     REGTTQPPGKQPNPDGGSSPSTGGG"
     gene            5462..5535
                     /locus_tag="VH419_08545"
     tRNA            5462..5535
                     /locus_tag="VH419_08545"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5496..5498,aa:Met,seq:cat)
BASE COUNT          974 a         1830 c         1949 g          907 t
ORIGIN      
        1 cgcccatccg acggcggagt cggtgctgcg tgaggtcgtg gagaaggtgg tcgcctcaca
       61 cgacgtacgg cgcgtcgcgg ccgtccaccg cgtcggggag ctggcgatcg gtgacgtggc
      121 ggtggccgtc gccgtgtcgt gtgaccaccg gggggacgcg ttcacagcct gccgggcgct
      181 catcgacgac ctcaaggcga ccgtgccgat ctggaagcac cagtggttcc gggacgggag
      241 cgacgagtgg gtcggcaccc cgtagaggtc cgctgaacag cgccacaggg tttcgccaag
      301 cctgtcctga gcctgtcgaa gggctcaacc ggcggatgtg ccacgtacgc tgaacgggca
      361 cgttgtgaca gccgggagat tcacgggagg cgcgatggcg gtgctgtggt ggttggtgcc
      421 accgctggtg accacatgcc tggccatggt ctgggtcggc tggctcgggc gcgaacgcga
      481 tgagacacgg cgggacgact ccgaggcggc tctccagcgc atgcagaaag cactgtcccg
      541 accggccccc accaagggca agccggtgat gtcgacgccg atcgaaccga cccacggcgt
      601 cgcgatccgc ggcgccgctc accgccctgt tgcgcccacg aagtcggcac ggtgagacga
      661 tcctccgcat gacacggcgc acgatcaccc tgctcggggc gggagtcgtg ctgggtgcgc
      721 tgctgctggt cgccttcttc gcgccgatgc cgtacgtcgt gatgtcgccg ggcctcaccg
      781 agaacacgct ggggtccttc gacggcaaac aggtcatcac gatcgacggc cacaagacct
      841 atccgacgtc gggacgactc gacctcacca cggtgtcggt gacttcgccg gactactcgc
      901 cacggctgag cgagatcctc caggcctggt gggacaagga cgagatcgtg atcccacgcg
      961 acgtcgccta cccaccgagc cagtcggtcg agcaggtcca gcagcagaac cgaaccgaca
     1021 tgctcgactc tcagcagagc gcgatcgcgg cgggactcgg tgaggcgggg atcccgtcgg
     1081 tgtatgtgac ggtcagcgac gtggagaagg acgctcccgc gaacggggtc ctgaagaccg
     1141 gcgacctgat cacgtctgtc gacggcacga cgatccattc cgtcgatgcg gccatctcgg
     1201 gcatctcgag cgtcgaaccg ggttccgacg tcacgttggg gatcaagcgc aacgacaagg
     1261 acgagcaggt cacgatcacc accgaggcat cggaagacga tccgtccaag gctcgtatcg
     1321 gcgtcggact cggtgagacg atcgatccgc cgttcacggt gaagatcaac ctcggtcaga
     1381 acatcggtgg accgagcgcc gggacgatgt tcgcgctggc gatctacgac atgatcaccc
     1441 ccggcccgct caccggtggt cgccacgttg ccgggaccgg gacgatcact ccggagggga
     1501 gggtcggcgt catcggcggg gtgcagcaga agatcgccgg cgcctaccgc gaaggggcca
     1561 ccgtcttcct cgtaccggca ggcgactgcg ccgaggcggc gaagtcgccg ttggccgatg
     1621 acatccagct ggtgaaggtc gagacgctcg accaggcggt caacgcgctc gagggcatcg
     1681 actccgggga cacctccgcg gtgacgccct gcacgacggg atgacagcga gcgacggcgg
     1741 tgacgtgagc cgccggaccg cgcggcagga cggcggggcg gcgtactccg gcgaggtctc
     1801 cgaacgcatg ctcgggctca gggtcgccgt ggtggaggtc gagcggcata cgagcgaggc
     1861 gggatgggat caggcggcgc ggttgttcgc cctggttcgt accgtcgacc tggctgccgc
     1921 ggagcccgag ctcgccgcag agctcggcat caacgacgga accgccgatc ttttcacgcc
     1981 gatcgagcag gaactcgacg agcacactgg gtcgttggag gagttgttgg gtcgcatcac
     2041 gtggcctgag accgtcgacg gtgcgttggc agtggtggag cggctggtgc tgccacccga
     2101 cgccgaggag gcggtacctg acgacccagg gaaggctgcg gagtttgccg ctggtcacca
     2161 cgcccgccag gacgtgcgga tggcggcggg agtcctgcgc tcgggggagg cgcattgcgt
     2221 cctccgggtg cgttcgcacg acagtgacga cgcgctgctg cacggcccgg acgtcgtgcc
     2281 cggtctggtc tcagcgttgc gtgagacgct tgaaccgtag aacgagccgc atccggcagg
     2341 cggctactgt tgccggacct gtacgaccgt tcggaggagt cccgtgagcg agatgttcga
     2401 ggaggaggcg cagccagagc ggccaccccg ccgccggcag ccctctcagc ggcctcgggc
     2461 actgctgccg acgctcggca tcgtcgtggc gctggtggtg ctcttctccg tgttcgtcga
     2521 ggtgtggacc gggcggctgt ggttccaggc gttgggctac caaggcgtct tcacgaaggt
     2581 gctgtggacg cggaccgcgc tgttcttcat cttcggcctg ctgctggccg gcatcgtggt
     2641 cggcaacgcg ctgctggcgt tccgggtgcg gccgatcctc ttcgccgacg gttaccgcaa
     2701 ccccaccgtg gagcgctacc aagacaccgt cgacccgatc cggcactggg tgctgatcgg
     2761 gcttggcgtg atcatgttcc tgttcggcgg agccagcgcg tccggggagt ggaagaccta
     2821 tctgttgtgg cagcaccggg tgccgttcgg ccagaacgac gtgtacttcg gcaaggacat
     2881 cggcttcttc gtgttcagct acccgtggta ccgattcctg atcagcttcg gcttcaccgc
     2941 gctcgtcatc gcgatgatcg tcaccgccgc aacgcactac ttgtacggcg gcatccgact
     3001 cggcgcgaag cgggagaagt tctcgcacgc cgctcaggtc cagctgtcgg tgctgttcgg
     3061 gttgttcatg ctgctcaagg cggtgtcgta ctggctcgac cgctacgggt tggcgatctc
     3121 cagcggccac ctgttcaccg gcgtctcgta tgccgacgcg aacgccgtgc tgcctgcgaa
     3181 gaacatcctc atgatcatcg cgttgatctg cgcgctgctc ttcttcggca acgtgatccg
     3241 gccggggtgg atgttgccgg tgctcggctt cggactgctc gtgctgtcgg ccatcctgat
     3301 cggcgggatc tggcccgcca tcgtgcagcg gttccaggtc aagccgtcgg aggcggacaa
     3361 ggaagcccgc tacatcgagc tcaacatcaa cgcgacccga gatgcgtatg ggctcaatga
     3421 gatcaagcct gtgtcatatc ccggcgtcac caccgacacg cccctgcagc tgcaaaagct
     3481 ggcgagtgag cttcccggcg tacgactcat cgaccccaag ctggtcgctc cggcgttcga
     3541 gcagctgcag cagcagcgcg gcttctacaa gatgccggat gtgctcgacg tcgaccgtta
     3601 caagttcacg ggagacgcgc agccgcagga cgtcgttctc gcagcgcgcg agctgagcct
     3661 caacggtctg cgcgacgacc agcgcaactg gacgaacgac cacactgtct acacgcacgg
     3721 ctatggcgtc gtggcggcgt acggcgacga gcgtggcagc gcgggtgagc cggtctgggc
     3781 ccagcagggg ctgccgtcgg agggccagct cggcgacttc cggcaggagg tctactacgg
     3841 cgagagcgag cccagctact cgatcgtggg tgctccggag gggacgccgc cggtcgagct
     3901 gaacatcccg gctgtaggga gcggcggcac cgacaccgac cagaactcca cctacgaggg
     3961 ggacggcggt gtgccgctcg gctcgacgtt gaaccaggtc ctgtacgccg cgaagttctg
     4021 ggactcctcc atcctgttgt ccggacgcgt caacccggag tcgaagatca tctacgaccg
     4081 gagcccgcga acgatggtcg agaaggttgc gccgtggctg acggtcgact ccgacacgta
     4141 ccccgcggtg gtcgacggcc ggttgaagtg gatcctcgac ggctacacga cgacgtcgga
     4201 ctacccgatg tctgagcgcg tcgacctgag cgagacgacg agtgactcac tgactgccga
     4261 agacgccgtc gcggcacaga agtccgacga catcaactac atccgcaact cggtgaaggc
     4321 cacggtcgac gcgtacgacg gcaccgtgac gctgtaccag tgggacgaga acgaccccgt
     4381 cctgcaggcg tggatgggcg ccttccccgg tgtggtgcag ccgaagtcgg agatctccga
     4441 tgacctgatg gcgcacctgc gttatccgga ggacctgttc aaggtgcagc gcgagctgct
     4501 ctcgacgtac cacgtgacca acccgaagac gttctacagc ggctcggaga actggcgggt
     4561 gcccgaggac ccgaacgccg acaacaccgc cgcgaagcag ccgccgtact tcctcaccgt
     4621 caagctgccc gaggacgtga cgtcggagac gcaccaggag agcgccccgg agttctcgct
     4681 gacgagcgtt tacgagccga acagccggca gaacctcgcc agcttcgtcg ccgtcaacgc
     4741 cgacgcgacc agtcctgact acggcaagtt caccgtgctc gagctgccga gcgacaacgc
     4801 cgtgagcgga ccgagccagg tcgcgaacgc gatgtcgaac gacccgaagg tgcgcaacga
     4861 gctgctgaag ttcacccagg tcggcacgaa ggtgctctac ggcaacctgc tgacgctgcc
     4921 ggtcggtgac gagctgttgt acgccgaacc ggtctacacg ttgcgcggtg gcaccgggac
     4981 cgggacgtat ccgatcctgc agttcgtcgt ggtctcgatc ggggacgacg tggggatcgg
     5041 cgactcgttc gaggcggcct tcgacagcgc attgaacctc gaagaggcga cgacgccgcc
     5101 gccgaccgag aatccgccgc caccgccgtc ccagggcggg aacggcaagc cgggccagca
     5161 gacgaccaac cagcagatca ccgccttgct cggggaagcc caggacttct tcacccgcgc
     5221 ccaggcggcg ctcaaccggg gtgacctcgg cgagtaccag cgactcaacg acaagggtta
     5281 cgccaagctg gctcaggcga tcgccctgcg cgaagggacg acgcagccgc ccggcaagca
     5341 gcccaacccg gacggcggct cgagtccgtc gaccggcggc gggtgacgcg caggtcacac
     5401 ggcgccgatt tgatctggct tggcgggctg cgtagtgttg accctcgcat gacgcacccg
     5461 acgcggggtg gagcagttcg gtagctcgct gggctcataa cccagaggtc gcgggttcaa
     5521 atcccgcccc cgctacttcg ctggcctggg ctttcgtccg ggccagttcc gttttcaggg
     5581 gtgaatctgc cgcaacggga gcggattcgg gagcgaagcc ttcgccgtta tcagtcgccg
     5641 acccaaggat gagctcggcg
//
LOCUS       DATBFZ010000275         1507 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_1824531, whole genome shotgun sequence.
ACCESSION   DATBFZ010000275 DATBFZ010000000
VERSION     DATBFZ010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1824531"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(148..1227)
                     /locus_tag="VH419_08550"
     CDS             complement(148..1227)
                     /locus_tag="VH419_08550"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEY7043702.1"
                     /translation="MTAADGAGQAVWETDEFRDRALTWIDAALAEHDLRRTGEVEERV
                     RNWAAVYRVPTDGGVFWFKAEGRGQAFEAPLYPLLQRLVPDRVLHPLAVDVQRAWLLL
                     PDGGTILRQTLGDGAEAADAMAGFLPEYARLQIELMPHVPELLAVGVVDMSPAVMLRR
                     FDEALVVAKRYVDVVVARGGETTSPHPSPRSAAEVQAAYDRIVERRDRVERQCRLLSN
                     GAVPPSLQHDDLHDGNVFATAPDAPPRFFDWGDAVVAHPFATLLITLRVLCDEAGVLS
                     DDPVVHQVRDAYLEPFGLYASHAELVELLEAACDVGKVTRSLTWARELRAMPADQAAE
                     FAANQFYWLEELTVDDYLGSPPDAD"
     gene            complement(1259..>1507)
                     /locus_tag="VH419_08555"
     CDS             complement(1259..>1507)
                     /locus_tag="VH419_08555"
                     /inference="COORDINATES: protein motif:HMM:NF036450.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5302 domain-containing protein"
                     /protein_id="HEY7043703.1"
                     /translation="RGIGVVTARKVCLHGTSRNDEESDMPAEKPDHEDEQHQKFREAL
                     ERKKAAGHDTPVHATTNKGVGEAHNDHTRREFRRKAGS"
BASE COUNT          224 a          547 c          508 g          228 t
ORIGIN      
        1 atcgcgtcca cctcgtcgac gggcatcggg aacagcccgg cgcggtacgc cgacaggatc
       61 gtccctggct cgaggtcggc gccgaccgcg accacatcgt cctcggcctc ccgcggatcc
      121 gggaacaccc aaggcgacgc cggtggctca gtcggcatcc ggcgggcttc ccaggtagtc
      181 gtcgacggtc agctcctcga gccagtagaa ctggttcgcg gcgaactccg ccgcctggtc
      241 ggcaggcatc gcccgcagct cgcgcgccca ggtgagcgag cgggtcacct tgccgacgtc
      301 gcaggcggcc tcgagcagct cgacgagctc cgcgtgcgac gcgtacagcc cgaacggctc
      361 gaggtaggcg tcgcggacct ggtggacgac agggtcgtcc gagagcacgc ccgcctcgtc
      421 acacagcacc cgcagcgtga tgagcaacgt cgcgaacgga tgcgcgacga cggcgtcgcc
      481 ccagtcgaag aaccgtggcg gagcatcggg tgcagtcgcg aagacgttgc cgtcgtgcag
      541 gtcgtcgtgc tgcaagctcg gcgggaccgc accgttcgac agcagccgac actgccgctc
      601 gacccgatct cgacgctcga cgatgcggtc gtacgccgcc tggacctcgg cggcggaccg
      661 tggcgacggg tgcggtgacg tggtctcgcc gccgcgggcc accaccacgt cgacgtacct
      721 cttcgccacg acgagcgcct cgtcgaaacg acgcagcatc acggctgggc tcatgtcgac
      781 gacgccgacg gcaagcagct cgggcacgtg tggcatcagc tcgatctgga gccgcgcgta
      841 ctcgggcagg aagcctgcca tggcgtcggc tgcctcggca ccgtcaccaa gggtctggcg
      901 gaggatcgtc ccaccgtctg ggagcagcag ccacgcccgt tgaacgtcga cggcgagcgg
      961 atggaggacg cggtccggca ccagccgctg caggagcggg tacagcggcg cctcgaacgc
     1021 ctgcccccga ccttccgcct tgaaccagaa cacaccaccg tcggtcggga cgcggtacac
     1081 cgcagcccag ttgcggaccc gctcctcgac ctcaccggtc cggcgcaggt cgtgctcggc
     1141 cagagcggcg tcgatccagg tcagggcgcg gtcgcggaac tcatcggtct cccacacggc
     1201 ctggccggca ccgtcagcgg cggtcaccgt ccagagtctg ccgtgcgcgt cgggtacctc
     1261 agctgccggc cttgcggcgg aactcccggc gggtgtggtc gttgtgcgcc tcgccgaccc
     1321 ccttgttcgt cgtggcgtgc acaggagtgt cgtgcccggc ggccttcttg cgctcgaggg
     1381 cctcgcggaa cttctggtgc tgctcgtcct cgtggtcagg tttctcagcc ggcatgtcgc
     1441 tctcctcgtc gttcctgctc gttccatgca agcaaacctt gcgcgcagtc accaccccaa
     1501 ttccccg
//
LOCUS       DATBFZ010000276         2221 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_5019490, whole genome shotgun sequence.
ACCESSION   DATBFZ010000276 DATBFZ010000000
VERSION     DATBFZ010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5019490"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..747
                     /locus_tag="VH419_08560"
     CDS             <1..747
                     /locus_tag="VH419_08560"
                     /inference="COORDINATES: protein motif:HMM:NF012892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporting ATPase C-terminal
                     domain-containing protein"
                     /protein_id="HEY7043704.1"
                     /translation="NDAAALHAADVGISVDTAVDVAKQAAANVLLDKSLAVVADGVRL
                     GRQTFANTLKYVRVTTSANFGNMLSMAAAAAFLPFLPLLPRQILLLNFLSDIPGTTIA
                     ADTVDPEQLARPRAWDIRSIRTFMIVFGLVSSVFDGLTFLALRLGFDAGAALFRSGWF
                     IESTATELAVMLVLRTSRPFWRSRPGHALVVTSVAVAAITVALPYTPLADPLGFVPVP
                     LRVLGTLAALTVIYVVANEIAKRRLRWAFA"
     gene            complement(771..1178)
                     /locus_tag="VH419_08565"
     CDS             complement(771..1178)
                     /locus_tag="VH419_08565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043705.1"
                     /translation="MSDGRTYVPFRHTRKAATHWATASDPAVLQPRFALRVMCPARHR
                     VHALFRVVCIVTTPFFVGLPGFRSKLWMVDPATGDFAGLYEWDDEPTARAYAEGLCKV
                     LRLLSVPGSVSYEVTPCCTVDEYLASADRIKAA"
     gene            complement(1350..2138)
                     /locus_tag="VH419_08570"
     CDS             complement(1350..2138)
                     /locus_tag="VH419_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020388264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HEY7043706.1"
                     /translation="MTVAVGGPKILVRRPGPRLADGIVTHVERVPVDVVLAHRQWEKY
                     VGVLEDSGWDPVEVPPADDCPDAVFVEDAVVVYRNIAVITRPGAPERRPEIVDVEKVV
                     EALGYSLNHIHAPGTLDGGDVLKIGRTVYVGRGGRTNADGVRQLRAILQPLDATVVAV
                     PVTKVLHLKSAVTALPDATVIGYRPSVDNARIFARFRAVPEESGAHVVLLGDSKLLMA
                     ADCPESAEAFADLGFDPVVVDISEFQKLEGCVTCLSVRLRATAA"
BASE COUNT          327 a          777 c          766 g          351 t
ORIGIN      
        1 aacgacgcgg ccgcgctcca cgcggccgac gtcggcatct cggtggacac ggccgtcgac
       61 gtcgcgaagc aggccgccgc caacgtgctg ctcgacaaga gcctcgccgt cgtcgccgac
      121 ggcgtgcgcc tcggccgcca gacgttcgcg aacacgctca agtacgtccg ggtcacgacg
      181 agcgccaact tcgggaacat gttgtcgatg gcggccgcgg ccgcgttcct gccgttcctg
      241 ccgttgctcc cgcgccagat cctgctgctc aacttcctct cggacatccc gggcacgacc
      301 atcgccgccg acaccgtcga cccggagcag ctcgctcggc cccgggcatg ggacatccgg
      361 tcgatccgga ccttcatgat cgtgttcggg ctcgtcagct ccgtcttcga cgggttgacg
      421 ttcctcgccc tgcgcctggg ctttgatgcc ggcgcagcgt tgttccgcag cggctggttc
      481 atcgagtcga ccgcgaccga gctcgccgtg atgctggtgc tgcgcaccag ccgaccgttc
      541 tggcgcagcc gcccgggcca tgcgttggtc gtgacgagcg tcgcggtcgc ggccatcacg
      601 gtcgcgttgc cgtacacgcc gctcgcggat ccgctcgggt tcgtgcccgt gccactgcgg
      661 gttctcggga cgctcgcggc gttgacggtg atctacgtgg tcgccaacga gatcgccaag
      721 cggcggctcc gctgggcctt tgcgtgagtc cggctgcggc gatcctctgc tcacgcagcc
      781 ttgatcctgt cggcgctcgc caggtactcg tcgacggtgc agcacggcgt gacctcgtac
      841 gagaccgacc ccggaacgga cagcaggcgc aagaccttgc agagtccctc ggcataggcc
      901 cgggcggtcg gctcgtcgtc ccactcgtag aggccggcga agtcaccggt ggccgggtcg
      961 accatccaca gcttcgaccg gaagcccggg agcccgacga agaacggcgt cgtgacgatg
     1021 cacacgaccc ggaagagcgc atgcacccgg tggcgagccg ggcacatgac ccggagcgcg
     1081 aagcggggct ggagaactgc ggggtcgctt gcagtcgccc aatgggtcgc cgccttgcgc
     1141 gtgtgccgga acggcacgta cgtgcgaccg tcgctcatgc gcaggggcct cccgagccgc
     1201 tcacggtggc agcgcgtggt cccccccaga agatcgcggg ccgcggccgg gaacgactgg
     1261 aaccattcgc gtggcccgat gtgtcgctcg accgccgctg cctccgagtt gacctgatct
     1321 gttgcaggtc gtgacctcat gacgacctcc tacgctgcgg tcgctcggag tcggaccgac
     1381 aggcaggtca cgcaaccctc gagcttctgg aactcgctga tgtcgacgac gaccgggtcg
     1441 aaacccaggt ccgcgaaggc ctcggcgctc tcggggcagt cggcggccat cagcagcttg
     1501 gaatctccga gcaggacgac gtgcgctccc gattcctctg gcacggcccg gaagcgggcg
     1561 aagatccggg cattgtcgac cgacggtcgg tagccgatga cggtggcgtc gggcagcgcc
     1621 gtgaccgccg acttgaggtg cagcaccttc gtcaccggga cggcgacaac ggtggcgtcg
     1681 agcggttgga ggatggcgcg gagctggcgc accccgtcgg cgttggtccg accgccgcgg
     1741 ccgacgtaga cggtgcggcc gatcttcagg acgtcgccgc catcgagcgt gcctggcgca
     1801 tggatgtggt tcagcgagta gccgagtgcc tcgaccacct tctcgacgtc gacgatctcg
     1861 ggtcgccgct ccggcgcgcc gggccgagtg atgacggcga tgttgcggta gacgacgacg
     1921 gcgtcctcga cgaacacggc gtccgggcag tcgtcagcgg gcgggacctc gaccgggtcc
     1981 cacccgctgt cttccagcac accgacgtac ttctcccact ggcgatgggc cagcaccacg
     2041 tcgaccggca cgcgttcgac gtgggtcacg atcccgtccg ccagccgggg acccggccgg
     2101 cggacgagga tcttgggccc accaaccgcg acggtcatca gatcgcgtcg ccgagctcgt
     2161 aggtcgggga caggtcgacc gtcgtgggga cttggccaag ccgctcacgg cgagccttca
     2221 g
//
LOCUS       DATBFZ010000277         2189 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_4121681, whole genome shotgun sequence.
ACCESSION   DATBFZ010000277 DATBFZ010000000
VERSION     DATBFZ010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4121681"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /locus_tag="VH419_08575"
     CDS             complement(<1..483)
                     /locus_tag="VH419_08575"
                     /inference="COORDINATES: protein motif:HMM:NF028174.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase dimerization domain-containing
                     protein"
                     /protein_id="HEY7043707.1"
                     /translation="MESQIPPAWQLIALMDGFVATQLVYVAAKLGIAEILADGPHSGA
                     ELADAVGADRQALTRVLRGLAAVGVLTEVGDDGFALTDVGRALGPLGAMALARGDLYY
                     PAAAGLLDAVRDGDTAFDRVYGASFFDHLAQHPDLEATFQGSMSGRSSQEARDVVAAY
                     D"
     gene            640..1383
                     /locus_tag="VH419_08580"
     CDS             640..1383
                     /locus_tag="VH419_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020574416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY7043708.1"
                     /translation="MSTTPTTGRVALVTGGSGGIGRAVVERLAADGFSVAVHYAGNQA
                     KADAIVECIHAMGGSAIAVGGDVADEQAMQSLFDQVETAFGGVDVVVNTAGIMILSSI
                     ADLNLDDLDRMHRTNVRGTIVVSQQAARRLRAGGAIINFSTSVTRTQFPGYGAYVASK
                     AAVESITLILARELRGKDITVNAVAPGPTATALFLDGKDEATVHNLSKAVPLERLGKP
                     EDIAETVGFLAGPARWVNGQVVFTNGGLA"
     gene            1397..2035
                     /locus_tag="VH419_08585"
     CDS             1397..2035
                     /locus_tag="VH419_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008360894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal ligand-binding domain-containing protein"
                     /protein_id="HEY7043709.1"
                     /translation="MTAPRRRRHNGEPSKPTDSPRRGRGRRPVADVRADVLRTVGEIL
                     LNEGLADLTIDRVARVSGVSKTTIYKWWPSKGALALDGYFHSVADTLAFPDTGDIRTD
                     VTTQLRSFVTVMTHTPGGRILTELIGQSQTDTDLAAAYRALYSSQRRRLAGERLQRAR
                     QAGQLRADIDVQVVVDQLWGAVYHRLLIPDEPVNGDFVDALIGNLFDGVARS"
BASE COUNT          390 a          749 c          702 g          348 t
ORIGIN      
        1 gtcgtacgcg gcgacgacgt cccgcgcctc ctgcgacgac cgcccggaca tcgagccctg
       61 gaacgtggct tcgaggtcgg gatgctgggc caggtggtcg aagaaactgg ccccgtagac
      121 gcgatcgaag gccgtgtcac cgtctcgaac tgcgtcgagc agccctgctg cggccggata
      181 gtaaagatct ccacgcgcca gagccatggc tcccaacggg cccagcgccc ggccgacgtc
      241 ggtcagggcg aatccgtcat cgcccacttc ggtcagcacg cccaccgcgg cgagccccct
      301 cagcactcgg gtcagtgcct gccgatcggc gccgacggcg tcggccagct cggcgccgct
      361 gtgcgggccg tccgcgagaa tctcggcgat gccgagcttc gccgccacgt agacgagttg
      421 ggtggcgacg aaaccatcca tcagagcgat gagctgccag gccggcggaa tctggctttc
      481 catgatcgac tccagggacc gggtatgtga gcgcgcttgt ctgtatcaaa gacgatgcgt
      541 cggcgtcgtg caatctaccg attggaccga tatggacagg gcgtacattt cctgattaaa
      601 tggacgcaga gttcatatcg agagatcgga agcagaagca tgagcaccac cccaaccacc
      661 ggccgagttg ctctggtcac ggggggctcc ggcggcatcg gccgggccgt cgtcgagcgt
      721 ctggctgcag acggattctc ggtagccgtg cactacgcgg gcaatcaggc caaggcggat
      781 gccattgtgg aatgcatcca cgccatgggg ggatcggcga tcgccgtcgg cggggatgtc
      841 gccgacgagc aggcgatgca gagcctcttc gatcaggtcg agaccgcatt cggtggtgtc
      901 gacgtggtcg tgaacacggc cgggatcatg atcctctcgt ccattgcgga cctgaacctg
      961 gacgatctcg accggatgca ccgcacgaac gtccgcggca ccatcgtcgt gtcgcagcag
     1021 gcagcgcgcc ggctgcgtgc aggtggcgcg atcatcaact tctctacctc ggtgacccgg
     1081 acgcagtttc ctggctacgg cgcctatgtc gcgagcaagg ccgcggtcga gagcatcacc
     1141 ttgatcctcg cccgcgagct gcggggcaag gacatcacgg tcaacgccgt ggcccccggc
     1201 cccacagcca cggccttgtt cctcgacggc aaggacgagg caaccgttca caacctgtcg
     1261 aaggccgttc cgctcgaacg tctcgggaag cccgaggaca tcgcggagac cgtcggcttt
     1321 ctcgcggggc cggcccgctg ggtcaatgga caggtcgttt tcaccaatgg cgggctcgcc
     1381 tgagcaggag gaattcgtga ctgcgcctcg gcgacgacga cacaacggcg aaccgtccaa
     1441 acccaccgac agcccgcgcc gcggacgcgg acgccgaccc gtggccgacg tccgggccga
     1501 tgtgctgcgc actgtcggcg agatcctgct gaacgaagga ctcgccgacc tcacgatcga
     1561 ccgggtcgcc cgtgtctcgg gcgtgagcaa gacgaccatc tacaagtggt ggccgtccaa
     1621 gggcgcgctc gccctggacg ggtacttcca cagcgtcgcg gacactctcg ctttccccga
     1681 caccggcgac atccgtaccg acgtcacgac ccagctgcgc agcttcgtca cggtgatgac
     1741 ccacaccccc gggggtcgca tcctcaccga gctcatcggc cagtcccaga ctgacaccga
     1801 cctcgccgcc gcataccggg ctctctactc ctcccagcga cgtcgacttg ccggcgagcg
     1861 cctgcaacga gcacggcagg ccggacagct ccgtgccgac atcgacgtac aagtcgtcgt
     1921 cgaccagctc tggggagccg tctaccaccg cctcctcatc cccgacgaac cggtgaacgg
     1981 cgacttcgtc gacgccctga tcggcaacct gttcgacggc gtcgcccgca gctgacccga
     2041 gcggcgcgag tctggccggt gcgctgaaaa tcccgacctg gaggtacgcc agcggggctg
     2101 gccgctcagc tgatgactct ggcagtgctc ggtgtcggga ttttcagcgc accaagtcgg
     2161 tccatgatca ggtagaggac gcgatactc
//
LOCUS       DATBFZ010000278         2979 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_1498410, whole genome shotgun sequence.
ACCESSION   DATBFZ010000278 DATBFZ010000000
VERSION     DATBFZ010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2979
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1498410"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..785)
                     /locus_tag="VH419_08590"
     CDS             complement(63..785)
                     /locus_tag="VH419_08590"
                     /inference="COORDINATES: protein motif:HMM:TIGR02206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HEY7043710.1"
                     /translation="MGHHFQMFSPTHWVMLAIFVAGIAPVVRLGRKDRGDADGALRAG
                     RVYAVVLVAVTLTAELMQFLPGEFDFETELPLNLCDFAWIPAAYALWTQSRTPATVIY
                     LWGLVLSTQALITPALDTGFPYPQFFGFWATHFLIVWSAIYIVWGLGIRPSWRGYRIT
                     VGITLVWLVGVFVFNEVVGTNYGYVNHKPSGHSVLDFFGPWPSYVAVEVVLVAAVWAL
                     MTWPWARDPEGRVSTRSTAGVR"
     gene            complement(1392..>2979)
                     /locus_tag="VH419_08595"
     CDS             complement(1392..>2979)
                     /locus_tag="VH419_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020576975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEY7043711.1"
                     /translation="SDMSSNTESGLPFEPVYGPDSLKGWDPEQQLGDPGSFPYTRGVY
                     SSMYTGRPWTMRQYAGFGTAAESNQRYHELIDAGTHGLSVAFDLPTQMGHDSDSPIAH
                     GEVGKVGVAIDSVEDMRLLFDGIPLDQVSTSMTINAPAALLMLLYELVGEQQGVRGEQ
                     LRGTIQNDVLKEYIARGTYIYPPQQSLRLITDIFTYCRREMPRWNTISISGYHMAEAG
                     ATPAQEIAFTLANGIEYVRAAVAAGQDVDEFAPRLAFFFVSRTTLLEEVAKFRAARRI
                     WASVMRDEFGAKNPKSMMLRFHTQTAGVQLTAQQPEVNLVRVAVQALAAVLGGTQSLH
                     TNSFDEAIALPTQKAARLALRTQQVIAYETDVTKTVDPLAGSYAVEKLTDELEESTRS
                     LMRSVEDLGGAVAAIEQGFQKNEIEKSAYRIAQEIDSGERTVVGVNRFTVDVDDPYEP
                     LRVDPAIEAQQAERLARLRTTRNDDELDTQLDALRSAAAGTDNVLVPMKAALAARGTV
                     GEVCNALRDVWGVYHPPDAF"
BASE COUNT          483 a          989 c         1003 g          504 t
ORIGIN      
        1 cgggtcaggc ggtcaccgga ccccggcggt cagcggaccc ggcggtcagc gaaccccggc
       61 ggtcagcgga ccccggcggt cgagcgtgtc gagacccgcc cttcgggatc gcgtgcccag
      121 ggccaggtca tcagcgccca gacggcagcg accagcacga cctcgacggc gacgtacgac
      181 ggccacgggc cgaagaagtc cagcacggag tggccggacg gcttgtggtt gacgtacccg
      241 tagttggtcc ccacgacctc gttgaacacg aacacgccga ccagccagac caacgtgatc
      301 ccgacggtga tccggtagcc ccgccacgac ggccggatgc cgaggcccca gacgatgtag
      361 atggccgacc acacgatcag gaagtgcgtc gcccagaagc cgaagaactg cggatagggg
      421 aacccggtgt caagtgccgg tgtgatcagt gcctgcgtcg agagcacgag tccccagagg
      481 tagatgacgg tcgcaggcgt cctgctctgc gtccagagcg cgtaggcggc ggggatccag
      541 gcgaagtcgc acaagttcag cggcagctcg gtttcgaagt cgaactcgcc gggcagaaac
      601 tgcatgagct cagcggtcaa cgtgacggcc accagcacga cggcgtacac ccgacccgct
      661 cggagcgctc cgtcggcgtc gcctcgatcc ttccggccga gcctgacgac cggtgcgatc
      721 cccgccacga agatcgcgag catgacccag tgggtgggcg agaacatctg gaagtgatgc
      781 cccatgcctc catggtgcgc ccgttcgttg gtcgcgcgag tcgagacgcc gggattctcg
      841 agctcgggac tgctcaggtt tcggtgggcg gagccggtcg cgatgagtag ccgtgttccc
      901 ccggcgctcc gtcggcgtcg gttcaggtgg ataggcagcg aacagcggga gacgttcgtg
      961 ccccctgagc cggtcggccg actttccgtt cctgttgcct gctgtgtagc tggcgggttt
     1021 cggacatcgc cgctcaaccc ctcacggttt gcgatgttca catccgaggc ccactgtgcc
     1081 catcgaaggt ttgcgatgtt tacacgcctt ttgtgctgta aacatcgcaa acctcaacgg
     1141 caccatgccc gattcgtgcg gtagacgtct tttcggaggc ttggtgggcc gcgccgaagc
     1201 acgggcaggg cgcagtcctg cccggtatcg cgtgcccgac cggtgcatgg aggctcacgg
     1261 gtcaaggccc gccgcaggcg gtcgcgtcag cgatcgcgca agcgaccctt gacgcgtgag
     1321 gaatgcaccg gtagcaggcg cggccaaacg aacgtgagga gctgacttcg ccaagcgcag
     1381 acagcgggac ctcagaaggc gtcgggtggg tggtagacac cccagacgtc acgcagcgcg
     1441 ttgcacactt ctccgacggt gccgcgtgcg gcgagggccg ccttcatcgg caccagcacg
     1501 ttgtcggtcc cagcggcggc ggagcgcagc gcgtccaact gcgtgtcgag ctcgtcgtcg
     1561 ttgcgggtcg tccgcaaccg cgccagccgc tcggcctgtt gcgcctcgat cgcggggtcg
     1621 acgcgcagcg gttcgtacgg gtcgtcgaca tcgacggtga accggttcac cccgaccacg
     1681 gttcgctcac cgctgtcgat ctcttgggcg atccggtacg ccgacttctc gatctcgttc
     1741 ttctggaagc cctgctcgat cgccgccacg gcgccgccca ggtcctccac cgatctcatc
     1801 aggctgcggg tcgactcctc cagctcgtca gtgagcttct ccacggcgta cgagcccgcc
     1861 agcgggtcga cggtcttcgt cacgtcggtc tcgtacgcga tgacctgttg ggtgcgcagt
     1921 gcgaggcgcg cggccttctg cgtcggtaac gcgatcgcct cgtcgaagga gttcgtgtgc
     1981 agcgactggg tgccgccgag tacggccgca agcgcctgca ccgccacccg cacgaggttg
     2041 acctccggct gctgggccgt cagctgtacg ccggcggtct gcgtgtggaa gcgcagcatc
     2101 atcgacttcg ggttcttcgc accgaactcg tcgcgcatga ccgacgccca gatacgccgt
     2161 gcggcgcgga acttcgcgac ctcctccagc agcgtcgtcc gggagacgaa gaagaacgcc
     2221 agccggggtg cgaactcgtc cacgtcctgt cccgcggcga cagcggctcg gacatactcg
     2281 atgccgttgg ccagggtgaa cgcgatctcc tgcgcaggtg tcgcgccggc ttcggccatg
     2341 tggtagccgg agatggagat cgtgttccaa cgcggcatct cgcggcgaca gtacgtgaag
     2401 atgtcggtga tcagccgcaa cgactgctgc ggcgggtaga tgtaggtccc gcgggcgatg
     2461 tactccttga gcacgtcgtt ctggatggtc ccccgcagct gttcgccgcg aacgccctgc
     2521 tgctcgccca ccagctcgta gagcagcatc agcagcgcgg ccggggcgtt gatcgtcatc
     2581 gacgtcgaca cctggtcgag cgggatcccg tcgaacagca gccgcatgtc ctcgaccgag
     2641 tcgatcgcga cacccacctt gccgacctct ccgtgcgcga tcggggaatc ggaatcgtgt
     2701 cccatctggg tcggtaggtc gaaggcgacc gagagtccat gggttccggc gtcgatcagc
     2761 tcgtggtagc gctggttcga ctccgcggcg gtgccgaacc cggcgtactg gcgcatcgtc
     2821 cacgggcggc cggtgtacat cgacgagtag acaccgcggg tgtacgggaa cgaccctggg
     2881 tccccgagct gctgttccgg gtcccagcct ttcagcgagt ccggtccgta gaccggttcg
     2941 aacggcagcc cggactcggt gttgctgctc atgtccgac
//
LOCUS       DATBFZ010000279         3410 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_4123941, whole genome shotgun sequence.
ACCESSION   DATBFZ010000279 DATBFZ010000000
VERSION     DATBFZ010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 3410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3410
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4123941"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..891)
                     /locus_tag="VH419_08600"
     CDS             complement(<1..891)
                     /locus_tag="VH419_08600"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY7043712.1"
                     /translation="MSLDLRESGNPAISEQVTIAAATSSVGRKNYPRLAWNWVWPKAL
                     AVGLVLLVWQIAIWTHWKPDYILPSPGTVFHQLWNEMTGDGRSQYTLWEQLNLTLKRG
                     ITGYALAILIGTSLGIAVVQWRTMRMAVGSLIAGLQTMPSIAWFPLAILFFGITEKAI
                     LFVVILGAAPSIAAGVISGIDEVPPPLLRAGQMLGARGANRYRYVVLPAAMPAYLAGL
                     KQGWAFSWRSLLAGELLVPIGGAASLGSALAYSRTVVNGAPWLLALMIVILVIGMVID
                     GIFGFFTRRMRLKRGLTGAVS"
     gene            complement(881..1735)
                     /locus_tag="VH419_08605"
     CDS             complement(881..1735)
                     /locus_tag="VH419_08605"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY7043713.1"
                     /translation="MTTINTTRKRTHTSSRSRGVTEATPRHAGAGPEANASGPAVRIR
                     GVSKTFGRGAEATTALQSVDLDVAEREFLCIVGASGCGKSTLLNLIAGLDMPTHGSID
                     VGGRKVAFMFQEATLFPWLTAGQNVDLALKLSGTPKRDRPARIDELFQLVHLGGRQNK
                     RPHELSGGMRQRTALARVLAQDAEIVLMDEPFAALDAMTRDSMHDEIERIHAELGLTI
                     VFVTHNVREAVRLSDRTVLFSSSPGRVKTEYAVDLPRPRHFDDIPVATLSGELTNELK
                     AEVAQHVA"
     gene            complement(1732..2814)
                     /locus_tag="VH419_08610"
     CDS             complement(1732..2814)
                     /locus_tag="VH419_08610"
                     /inference="COORDINATES: protein motif:HMM:TIGR01728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY7043714.1"
                     /translation="MIGHLTGKRLHRLGALVAAGVIATSLAACSSDDGDDNGGGGGGG
                     SSTEVKLGYFPNLTHASAIVGIEKGYFKDELAKDNATVKTLDFNSGSDTIDALNNGDL
                     DATYIGPSPAITAFATSHNVSVISGATSGGASLVVNKDIKSAKDLAGKTLATPGAGNT
                     QDVALKYWLKEQGYDVNPDGQGDVTVINQDNSLTVQAFGQGQVDGAWLPEPYASLLID
                     EGATKLVDEKDLWPGGEFVTTHLIVNNDFLKDHPDLVDDLLQGQIESNDYIAENPDDA
                     KTLVGGYIGKLTGSQVPANVLDSAWSQLTFTNDPIADSLLTSADHAAEVGLIDPVDDL
                     ADIYDLDPLNKLLAGAGESQVSGPSQ"
     gene            2971..>3410
                     /locus_tag="VH419_08615"
     CDS             2971..>3410
                     /locus_tag="VH419_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020386334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3417 domain-containing protein"
                     /protein_id="HEY7043715.1"
                     /translation="MRAIRRFTVRPVLPESLRQLEELAINLRWCWHLETQDLFEAVDA
                     DAWAASHHDPVRFLGAVPASRLQALAADKKFTRRLELAKADLDEYMTGERWYQSLGAD
                     VPVSIAYFSPEFGIAAVLPQYSGGLGILAGDHLKSASDLGVPILG"
BASE COUNT          542 a         1112 c         1145 g          611 t
ORIGIN      
        1 gctgaccgct ccggtcaggc cccgtttgag gcgcatccgt cgggtgaaga agccgaagat
       61 gccgtcgatc accatgccga tgacgaggat gacgatcatc agcgccagca gccacggcgc
      121 gccgttgacg acggtgcgcg agtacgccaa tgcactaccc aaagaggccg cgccgccgat
      181 ggggaccaac agctcaccgg ccagcagcga acgccaggag aatgcccagc cctgcttgag
      241 acccgccagg tacgccggca tcgcggcggg caggacgacg taccggtacc ggttggctcc
      301 ccgcgccccg agcatctgac cagcgcgcag caatggtggc gggacctcgt cgatcccgga
      361 gatgacgccg gcagcgatgg acggagcggc gcccaggatc accacgaaca ggatcgcctt
      421 ctcggtgata ccgaagaaca ggatcgccaa cgggaaccag gcgatcgacg gcatggtctg
      481 cagaccggcg atcagcgaac ccaccgccat ccgcatggtg cgccattgca ccacggcgat
      541 gccgagcgag gttccgatga ggatggcgag cgcgtatccg gtgatgccgc gcttcaaggt
      601 caggttcagc tgttcccaga gtgtgtactg actacgtccg tcgccggtca tctcgttcca
      661 caactgatgg aacacggtcc cggggctggg caggatgtag tcgggcttcc agtgggtcca
      721 gatcgcgatc tgccagacga gcaggaccag cccgacggcg agcgccttcg gccagaccca
      781 gttccaggcc agccgcgggt agttcttccg cccgaccgac gacgtcgcag cggcgatcgt
      841 cacctgttcg ctgatcgccg ggttgccgga ttcacgaagg tcaagcgaca tgctgagcca
      901 cctccgcctt cagctcgttc gtcagctcac ccgaaagggt ggcgacgggg atgtcgtcga
      961 agtggcgtgg ccgcggcagg tcgacggcgt actccgtctt gacgcgacca ggtgagctcg
     1021 agaacagcac cgtccggtcg gagagtcgaa ccgcctctcg gacgttgtgc gtgacgaaga
     1081 cgatcgtcag gccgagctcg gcatggatcc gctcgatctc gtcgtgcatg gagtcgcgtg
     1141 tcatcgcgtc cagtgccgcg aacggctcgt ccatgagcac gatctcggcg tcctgggcga
     1201 gcacccgagc caacgcggtg cgttgccgca tgccaccgga gagctcatgc ggccgcttgt
     1261 tctgccgccc accgaggtgg acgagctgga acagctcgtc gatcctcgcc ggccggtccc
     1321 tcttcggcgt accgctgagc ttcagcgcga gatcgacgtt ctggccggcg gtcaaccacg
     1381 gaaacagggt cgcctcctgg aacatgaacg cgaccttgcg gccgcccacg tcgatcgaac
     1441 cgtgcgtcgg catgtccagc ccggcgatga ggttgaggag tgtgctcttg ccacagccgc
     1501 tggctccgac gatgcagaga aactcccgct ctgcaacgtc gaggtcgacc gattgaaggg
     1561 ccgtggtcgc ctccgcacca cgaccgaacg tcttgctgac accgcggatc cggacggccg
     1621 gaccggaggc gttcgcctcc ggtccggcac cagcgtggcg gggggtcgct tccgtcacgc
     1681 cgcggctgcg agagctggtg tgagtccgct tgcgggtggt gttgatcgtt gtcactggct
     1741 cggtcctgat acctggctct cgcccgcgcc ggcaagcagc ttgttcagcg ggtcgaggtc
     1801 gtagatgtcg gcgaggtcgt cgaccggatc gatgagcccg acctcggccg cgtggtccgc
     1861 gctggtcagc agcgagtcgg cgatcgggtc gttggtgaac gtcagctggg accaggcgga
     1921 gtcgagaacg ttcgccggga cctggcttcc ggtcagcttg ccgatgtagc cgccgacgag
     1981 tgtcttggcg tcgtcggggt tctcggcgat gtagtcgttg gactcgatct ggccctggag
     2041 caggtcgtcg acgaggtccg ggtggtcctt gaggaagtcg ttgttgacga tcaggtgcgt
     2101 tgtcacgaac tcaccgcccg gccacaggtc tttctcgtcc acgagcttcg tcgcgccctc
     2161 gtcgatgagc agcgaggcgt acggctcggg cagccacgca ccgtcgacct ggccctgccc
     2221 gaacgcctgg accgtcaggc tgttgtcctg gttgatgacc gtcacgtcgc cctggccgtc
     2281 agggttcacg tcgtagccct gctccttcag ccagtacttg agcgcaacgt cctgggtgtt
     2341 gccggcgccg ggcgtggcca gcgtcttgcc ggccaggtcc ttggcactct tgatgtcctt
     2401 gttcacgacc agcgaggcgc cgccggacgt cgcgccgctg atgacgctga cgttgtggga
     2461 cgtggcgaac gccgtgatgg ccgggctcgg gccgatgtag gtcgcgtcga ggtcgccgtt
     2521 gttcaacgcg tcgatcgtgt cgctgccgct gttgaagtcg agggtcttga ccgtcgcgtt
     2581 gtccttggcg agctcgtcct tgaagtagcc cttctcgatg ccgacgatgg cactggcgtg
     2641 ggtcaggttc gggaagtagc ccagcttgac ctcggtcgag ctgccgccgc caccgccgcc
     2701 gccgttgtcg tcgccatcgt ccgagctgca cgccgcaagc gacgtcgcga tgacccccgc
     2761 cgcgacgagg gcgcccagcc ggtgcaggcg cttcccagtg aggtgaccga tcactgtgtc
     2821 tccttgagat gtaaagttta gtgaactacg cgagttagag tacatgaacg atgaggaggt
     2881 agtggtcgga tcccgaaatc cgggtgtgtc tgctcccaca gcctcccgat tcggtttccg
     2941 gcgatgcagc ggcgccgata ccgttgtccg gtgcgcgcca tccgacggtt caccgtccgt
     3001 cccgtcctgc ccgagtcgct gcggcaactc gaagaactgg cgatcaacct ccgctggtgc
     3061 tggcacctgg agacacaaga cctgttcgag gcggtggacg cggacgcgtg ggctgcctcc
     3121 caccacgacc cggtccgttt cctcggggct gttcccgcca gccggctgca ggcgttggcc
     3181 gcggacaaga agttcacccg tcggctcgag ctggccaagg cggacctcga cgagtacatg
     3241 acgggcgaac gttggtacca gtcgttgggc gcggacgtgc ccgtctcgat cgcgtacttc
     3301 tcacccgagt tcggcatcgc ggcagtgctg ccgcagtact ccggtggcct cggcatcctc
     3361 gccggtgacc acctcaagtc cgcgagcgac ctcggtgtcc cgatcctcgg
//
LOCUS       DATBFZ010000280         4333 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_1499804, whole genome shotgun sequence.
ACCESSION   DATBFZ010000280 DATBFZ010000000
VERSION     DATBFZ010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 4333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4333
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1499804"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /locus_tag="VH419_08620"
     CDS             complement(<1..441)
                     /locus_tag="VH419_08620"
                     /inference="COORDINATES: protein motif:HMM:NF040060.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6318 family protein"
                     /protein_id="HEY7043716.1"
                     /translation="MRRAFAAAVALLCLVGCTDDPADPTRTTTSSPTETSTTSQPPTT
                     TGTPTDAPPELPDAAKAKTTAGAKAFVRHYVDVLNYSWRFLDGRPLAEVSAADCEPCR
                     LIVKKIQQTAQNGGSQEGGFWTIKKIYALPGSTRATKSFLAVIHI"
     gene            complement(529..1251)
                     /locus_tag="VH419_08625"
     CDS             complement(529..1251)
                     /locus_tag="VH419_08625"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEY7043717.1"
                     /translation="MSGGPVIGITSYVEPASWSVWKDVPAALVPHGYVRHVHEAGGLA
                     VVVPPLPDDATEHDAREVLSRLDGLILAGGVDVEPSRYGQQSHPTIQLPRPDRDASEL
                     LLADLTARLDVPVLGVCRGMQVMAVAAGGELEQHLPDRIGHTGHSPAPGLYGEHPVEL
                     APGSRMRKVLGDQVTVATYHHQGVASFPGYTAAGWSTDGVVEAFEDGGARFRIGVQWH
                     PEVGVDSRLFEALVEACRATRS"
     gene            complement(1370..2152)
                     /locus_tag="VH419_08630"
     CDS             complement(1370..2152)
                     /locus_tag="VH419_08630"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020392484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEY7043718.1"
                     /translation="MVDVAEAGRLEGRTAVVTGGCSGIGLATVRRFAAEGAHVVVGDV
                     DDERGPGVADEVDGTYVHCDVTNADEVEAMFATAQSTYGSVDVAFNNAGISPPDDDSI
                     LDTGIDAWRRVQDVNLTSVYLCCKAVIPYMQRHGRGSIINTASFVAVMGAATSQISYT
                     ASKGGVLAMSRELGVQFARDGIRVNALCPGPVNTPLLRELFAKDPERAARRLVHIPLG
                     RFAEPEEIAAAVAFLASDDSSFITAAQFLVDGGIAGAYVTPL"
     gene            complement(2145..3521)
                     /locus_tag="VH419_08635"
     CDS             complement(2145..3521)
                     /locus_tag="VH419_08635"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003090658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY7043719.1"
                     /translation="MSTTHVVIDPATEEEVTQVQLATVEDTDEHIAKAKAAYDSWRHV
                     APADRGRLLRRFADAVDGAIDELAHLEVRNAGHTIGNARWEAGNVRDVLAYYSAAPER
                     MFGRQIPVAGGLDVTFKEPLGIVGVIVPWNFPMPIAGWGFAPALAAGNCVVLKPAELT
                     PLTALRIGEVALEAGIPEGVFTVIAGKGSIVGERFVTHPDVRKICFTGSTEVGKRIMA
                     GAADQVKRVTLELGGKSANIVFADSDLEQAAATAPYGVFDNAGQDCCARSRILVERSV
                     HDRFLELLEPAVQGVKVKPPDEDDAEMGPLISAGQRETVTSYVEGDSVDIAFRGDAPT
                     GAGFWYPPTVVLPRTEQDRVWREEVFGPVVAVLPFDDEADAIAKANDTQFGLSGSIWT
                     RDVGRAIRVSRAVETGNLSVNSHSSVRYSTPFGGFKQSGIGRELGPDALDAFTDVKNV
                     FISDGAGG"
     gene            complement(3649..>4333)
                     /locus_tag="VH419_08640"
     CDS             complement(3649..>4333)
                     /locus_tag="VH419_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HEY7043720.1"
                     /translation="RTADQHSVYKNAAKEIASQHGHAITFMAKYDEREGNSCHIHMSA
                     RGEDGSIVFDEGDGPTQTFEHFVAGIQATLYDFTLLDAPNINSYKRFAPGSFAPTAIA
                     WGRDNRTCALRVVGHGPSLRVENRLPGGDVNPYLGLAGMLAGGLYGIENELALEPAYE
                     GNAYESDKRHVPRTLQEARDAFGASKVARSTFGDAVVDHYLNNADVELAAFNTAVTDW
                     ERFRSFERM"
BASE COUNT          672 a         1517 c         1427 g          717 t
ORIGIN      
        1 gatatgtatg actgccaaga aacttttggt ggctcgggtc gagcccggca aagcgtagat
       61 cttcttgatc gtccagaagc cgccctcttg gctgcctcca ttctgggctg tctgttggat
      121 cttcttcaca atcaggcggc acggttcaca gtccgccgca gagacttccg cgagcggccg
      181 tccatcaagg aagcgccacg aatagttgag gacatccacg tagtggcgga cgaaggcttt
      241 ggcgccggcg gtggtcttgg ccttcgcggc gtccgggagt tccggcggcg cgtcggtcgg
      301 tgtgccggtg gtggttggcg gctggctcgt cgtggacgtc tcggtcggcg acgaggtcgt
      361 ggtacgcgtc gggtctgccg ggtcgtccgt gcaaccgacc aggcacagca gcgcgactgc
      421 ggcagcgaac gctcgacgca tacctgacct ccgggtgcaa cgaagtaccc ccaaactagg
      481 ggatctcgtg cacgcacgac cagaccgggc cggcgatctg tggacaactc aggagcgggt
      541 ggcccgacac gcctccacca atgcctcgaa cagccgcgag tccacgccga cctcgggatg
      601 ccactggaca ccgatccgga accgcgcgcc cccgtcctcg aacgcctcca cgacgccgtc
      661 ggtcgaccaa cccgctgccg tgtagccggg gaacgacgcc acaccttggt ggtggtacgt
      721 cgcgacggtc acctggtcgc cgaggacctt gcgcatgcgg cttccggggg cgagctcgac
      781 cggatgctcg ccgtacagcc ctggtgcggg ggagtggccg gtgtgtccga tccggtccgg
      841 caggtgctgt tcgagctcgc cgccggccgc cacggccatg acctgcatgc cgcgacacac
      901 gccgagcacc ggcacgtcga gccgtgcagt gaggtcggcc agtagcagct cggacgcatc
      961 gcggtccggc cgcggcagct ggatggtcgg gtgcgactgc tgtccgtagc gggacggctc
     1021 gacgtcgacg ccgccggcca ggatcaaccc gtccaaccgt gacagcacct cgcgtgcgtc
     1081 gtgctcggtg gcgtcgtcgg ggagtggcgg cacgacgacc gcgagtccac ccgcctcatg
     1141 gacatgccgg acatacccgt gtggcaccaa cgcggccggt acgtccttcc acaccgacca
     1201 ggacgccggc tcgacgtacg acgtgatccc gatgaccggc ccgccactca tcgccgagct
     1261 cccgccgtcg gatggcttcc gcgaattgcg ggaaaagtgc tgcccgaatg ctgctcaaaa
     1321 cctgatgttt cccgcaatcc gcggggaccc cgagcgagcc gagtcatggt cagagggggg
     1381 tgacgtaggc gccggcgatg ccaccgtcga cgaggaactg tgcggcggtg atgaagctcg
     1441 agtcgtcact ggccaggaac gccaccgcgg cggcgatctc ctccggctcc gcgaaccgtc
     1501 ccagcgggat gtgcaccagg cgacgtgccg cgcgctccgg atccttcgcg aacagctcgc
     1561 gcagcagcgg cgtgttcacc ggccccgggc agagcgcgtt gacccggatg ccgtcacggg
     1621 cgaactgcac gccgagctca cgtgacatcg ccagcacgcc acccttgctg gccgtgtacg
     1681 agatctggga cgtcgccgcg cccatcaccg cgacgaacga cgcggtgttg atgatcgagc
     1741 cgcgcccgtg ccgctgcatg tacgggatca ccgccttgca gcacagatag acgctggtca
     1801 ggttgacgtc ctgcacccgc cgccacgcgt cgatgccggt gtcgaggatc gagtcgtcgt
     1861 cgggcggtga gatcccggcg ttgttgaacg ccacgtcgac gctgccgtac gtcgactggg
     1921 cggtcgcgaa catcgcctcg acctcatcgg cgttggtgac gtcgcagtgc acgtacgtgc
     1981 cgtcgacctc gtccgcgacg ccggggccgc gctcgtcgtc gacatcaccg accaccacgt
     2041 gcgccccctc ggcggcgaag cgacgtaccg tcgccagacc gatgccgctg caccctccgg
     2101 tgacgaccgc ggtgcggccc tcgagacgtc ctgcttcagc cacgtcaacc accagctccg
     2161 tcggagatga agacgttctt gacgtcggtg aaggcgtcca gcgcatcggg gccgagctcg
     2221 cggccgattc cggactgctt gaacccaccg aacggcgtcg agtagcggac cgacgagtgg
     2281 gagttgacgc tcaggttgcc ggtctcgacg gcccgcgaca cgcggatcgc gcgaccgacg
     2341 tcacgcgtcc agatggagcc ggacagaccg aactgcgtgt cgttcgcctt cgcgatcgcg
     2401 tccgcctcgt cgtcgaacgg cagcaccgcc accaccggcc cgaacacctc ttcgcgccag
     2461 acccggtcct gctccgtgcg cggcagcacc accgtcggcg ggtaccagaa gccggcgccg
     2521 gtcggtgcgt ctccgcggaa ggcgatgtcc accgagtcgc cttcgacgta cgacgtcacg
     2581 gtctcgcgct ggccggcgct gatcagcgga cccatctcgg cgtcgtcctc gtccggtggt
     2641 ttgaccttga cgccttgcac agcgggctcg agcagctcga ggaatcggtc gtgcacgctg
     2701 cgttcgacga ggatgcggct tcgcgcgcag cagtcctgac cggcgttgtc gaagacgccg
     2761 tacggcgctg ttgcggcagc ctgctcgagg tcggagtcgg cgaacacgat gttggcgctc
     2821 ttgccaccga gctcgagcgt cacccgcttc acctggtcgg ccgcacccgc catgatccgc
     2881 ttgcccacct cggtcgaccc ggtgaagcag atcttgcgga cgtcgggatg cgtcacgaag
     2941 cgctcgccga cgatcgaccc cttcccagcg atcacggtga agacaccttc cgggatgccc
     3001 gcctccagtg cgacctcacc gatccgcagc gcggtgagcg gcgtgagctc ggccggcttc
     3061 aacacgacgc agttccctgc cgcgagcgcc ggcgcgaaac cccaaccggc aatcggcatc
     3121 gggaagttcc acgggacgat cacgccgacg atgccgagtg gctctttgaa cgtcacgtcc
     3181 aggccgccgg ccaccgggat ctgccgaccg aacatccgct ccggtgcggc ggagtagtac
     3241 gcaaggacgt cgcgcacgtt gcccgcctcc cagcgggcgt tgccgatggt gtgcccggcg
     3301 ttgcggacct ccaggtgcgc gagctcgtcg atcgcgccgt cgacggcgtc ggcgaagcgg
     3361 cgcagtaacc gaccgcgatc ggccggcgcg acgtgacgcc aggagtcgta cgccgccttc
     3421 gccttcgcga tgtgctcgtc ggtgtcctcg acggtggcga gctggacctg cgtgacctct
     3481 tcctccgtgg ccgggtcgat caccacatgc gtcgtactca cgttccgcct tctctcctgt
     3541 cctgcatcgt tcgtcccgtg gaggttcgcc tggttagcca tcccaatgga cggctaacca
     3601 ggcaaaccgt cctagggagg cggtgaactg cctcggtccc gaggcttctc acatgcgttc
     3661 gaagctgcgg aaccgttccc agtcggtgac cgcggtgttg aacgccgcca gctccacgtc
     3721 ggcgttgttg aggtagtggt cgaccaccgc gtcgccgaac gtcgaacgcg ccaccttcga
     3781 cgcgccgaac gcgtcccgcg cctcctgcaa ggtccgggga acgtgccgct tgtccgactc
     3841 gtaggcattg ccttcgtacg ccggttcgag cgccaactcg ttctcgatcc cgtacaaccc
     3901 tcccgccagc atcccggcaa gcccgaggta cgggttcacg tcgccaccgg gcaaccggtt
     3961 ctccacgcgc agcgacggac cgtggccgac gacccgcagc gcgcacgtcc ggttgtctcg
     4021 cccccacgcg atcgcggtcg gcgcgaagga cccaggcgcg aaccgcttgt aggagttgat
     4081 gttcggcgcg tcgagcagcg tgaagtcgta cagcgtggcc tggatgccgg cgacgaagtg
     4141 ctcgaacgtc tgcgtcggtc cgtcgccctc gtcgaagacg atggagccgt cctcacctcg
     4201 cgcgctcatg tggatgtggc aggagttgcc ctcccgctcg tcgtacttcg ccatgaacgt
     4261 gatcgcgtga ccgtgctgtg aggcgatctc cttcgcggcg ttcttgtaca ccgagtgctg
     4321 gtcagcagtc cgc
//
LOCUS       DATBFZ010000281         1683 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_4364548, whole genome shotgun sequence.
ACCESSION   DATBFZ010000281 DATBFZ010000000
VERSION     DATBFZ010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1683
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4364548"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="VH419_08645"
     CDS             <1..181
                     /locus_tag="VH419_08645"
                     /inference="COORDINATES: protein motif:HMM:NF019998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HEY7043721.1"
                     /translation="YVGMVTRYLVDLERGGELQVVRQNLETSSTEALEQQGRQVTVGW
                     SEEHTYAIPKDEEGT"
     gene            178..1374
                     /locus_tag="VH419_08650"
     CDS             178..1374
                     /locus_tag="VH419_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008359733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY7043722.1"
                     /translation="MRDARWIALVAVTCAMAVVVGCGGDDSGGSSSGSVKAPEGPKAP
                     DKLGAGEGEVDLIAWAGYVEDGSTDPKVDWVSDFEKDTGCQVKVKIGNTSDEMVTLMR
                     TGQYDGVSASGDATLRLIAGGDVAPVNTDLVPNYADVFDGLKDQPWNSVDGTPYGIPH
                     GRGANLLMWRSDKVKPAPDSWGAVFDANSPYKGKVTAYDNPIYIADAALYLKATQPDL
                     GIDNPYELDDKQFQASVDLLKQQRGIVGEYWSDYTKEQAAFTSGDSVLGTTWQVIANL
                     LDADKVPVKTILPKEGATGWSDTWMIASKAKHPNCMYMWMNHIISPKANAQVAEWFGE
                     APANKKSCQETADPNHCKTFHAEDEDYFSKVSYWTTPVKDCGDDRGAVCKDYSEWVQA
                     WTEIKG"
     gene            1443..>1683
                     /locus_tag="VH419_08655"
     CDS             1443..>1683
                     /locus_tag="VH419_08655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY7043723.1"
                     /translation="MIAYLGSLAVLFLAAFWQLDTFSGKVVHSYGVQNFETLIHEGVY
                     RSIVLRTVLIAAAVTVTDALLAFPIAFYMAKVARRR"
BASE COUNT          307 a          536 c          593 g          247 t
ORIGIN      
        1 ctacgtcggg atggtcacgc ggtacctcgt cgacctcgag cgcggcggcg agctgcaggt
       61 ggtgcggcag aacctcgaga cgtcctcgac cgaggcgctc gagcaacagg ggcggcaggt
      121 cactgtcggg tggagcgagg agcacacgta cgcgataccg aaggacgagg agggcacgtg
      181 agggatgcga gatggatcgc gctggtcgcg gtcacttgcg ccatggccgt ggtggtcggc
      241 tgtggcggtg acgactccgg tgggtcgtcg agcggttcgg tcaaggcgcc cgaaggcccg
      301 aaggcgccgg acaagctcgg cgccggtgag ggtgaggtcg acctcatcgc ctgggccggc
      361 tacgtcgagg acggctcgac cgacccgaag gtcgactggg tgtcggactt cgagaaggac
      421 accggctgcc aggtcaaggt gaagatcggc aacacgtcgg acgagatggt cacgctgatg
      481 cggaccggcc agtacgacgg cgtgtcggcc tccggcgacg cgacgctgcg cctgatcgcg
      541 ggcggcgacg tcgcgccggt gaacaccgac ctggtcccga actacgccga cgtcttcgac
      601 ggcctgaagg accagccgtg gaacagcgtc gacggcacgc cctacggcat cccgcacggc
      661 cgcggcgcca acctgctgat gtggcgcagc gacaaggtca agccggcgcc cgactcgtgg
      721 ggcgcggtgt tcgacgccaa ctcgccctac aagggcaagg tcacggcgta cgacaatccg
      781 atctacatcg ccgacgcggc cctgtacctg aaggcgacgc agcccgacct tgggatcgac
      841 aacccgtacg agctcgacga caagcagttc caggcgtcgg tcgacctcct caagcagcag
      901 cgcgggatcg tcggcgagta ctggtccgac tacaccaagg agcaggccgc gttcacgagc
      961 ggcgactccg tgctcggcac gacctggcag gtgatcgcga acctgctcga cgccgacaag
     1021 gtgccggtca agacgatcct cccgaaggag ggcgcgaccg ggtggtcgga cacctggatg
     1081 atcgcgtcca aggccaagca cccgaactgt atgtacatgt ggatgaacca catcatctcg
     1141 ccgaaggcga acgcccaggt ggcggagtgg ttcggtgagg cgccggccaa caagaagtcg
     1201 tgccaggaga ccgccgaccc caaccactgc aagaccttcc acgcggagga cgaggactac
     1261 ttctcgaagg tgtcctactg gacgacgccg gtgaaggact gcggtgatga ccgcggagcc
     1321 gtctgcaagg actactccga atgggtccaa gcgtggacgg aaatcaaggg ctagcggcaa
     1381 ccctctggcg tcggccccgc ctgcagctgt cactgctgct ggcggggccg atcggctggc
     1441 tggtgatcgc ctacctaggc tcgctggccg tgctgttcct ggccgccttc tggcagctcg
     1501 acaccttctc cgggaaggtc gtccacagct acggcgtcca gaacttcgag acgctgatcc
     1561 acgagggcgt ctaccgctcg atcgtgctgc ggacggtgct gatcgccgcg gcggtgaccg
     1621 tgaccgacgc gctgctggcg ttcccgatcg cgttctacat ggcgaaggtc gcccggcggc
     1681 ggg
//
LOCUS       DATBFZ010000282         8100 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_852253, whole genome shotgun sequence.
ACCESSION   DATBFZ010000282 DATBFZ010000000
VERSION     DATBFZ010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 8100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8100
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_852253"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..928)
                     /locus_tag="VH419_08660"
     CDS             complement(<1..928)
                     /locus_tag="VH419_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY7043724.1"
                     /translation="MDFHSEVTLALEQGLGPSAIIICLAAVGLNVHFGYTGLLNFGQA
                     AFMAVAGYGVASMVATWGLNFWFGCLVGIGLCIVLALLLGIPTLRLRADYLAIVTIAA
                     AEIIRFVIGASSLREYFGGQDGLQQYTSAFYDLNPYNGEVNILGLSWSRADFWVMTVG
                     WALVAICCVAIYLLMRSPWGRVLKGIREDEDAVRSLGKNVYAYKMQSLMIGGVIGGFA
                     GIVSGLYQSVQPTNFATDITFYAWTVLLLGGAARVVGPILGAVIFWFLISFLVNFFDD
                     ATQPPDPLIPASIMDPQQASLMRFIFLGLALML"
     gene            complement(943..2331)
                     /locus_tag="VH419_08665"
     CDS             complement(943..2331)
                     /locus_tag="VH419_08665"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY7043725.1"
                     /translation="MRVRLTVLAATVFAAVIAVVSLVWFAAPAQASAGSGLSDQAAAT
                     APSRCPHVQIPAKEDTIQIVGQLSDTRTNPCTPVPGVTITVIDQSDGSIIGQAESGKN
                     GTFVVPLPGAAIDNLGKTITVKIDTDTLPEGSSLTNPEDVENTKRIVIEGNISFTFPI
                     GPDLTPSTPWYETALQDLVTGLIFSSILALAALGLSMIFGTTGLTNFAHGEMVTFGAL
                     VAYAFDAGWGLPVILAGILATIVSGVFGWAQDAGLWRPLRRRGTGLIPMMIVSIGLSI
                     FLRSVYQYIAGADNHNYTQYATVEPWSIGTVDITPKELIVTVMAVAVLIAVTQALQRT
                     RMGKATRAVADNPALASSSGINVNRVISVVWIGGGALAGLSGFMLGLTQGFDYQLGFK
                     ILLLVFAAVVLGGLGTIWGAILGSVIVGVFIEMSTLIIPSELKFAGALAVLIVVLLFR
                     PQGLLGRAERVG"
     gene            complement(2501..3247)
                     /locus_tag="VH419_08670"
     CDS             complement(2501..3247)
                     /locus_tag="VH419_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HEY7043726.1"
                     /translation="MFRGFGTVVNVATVLLGSSIGVLLGHRLSQRTRDVVTDALGLVT
                     LLVAALSAFAVTDPDLRDAVGDSAPVLIVLGSLLIGGIIGSLVQIEYRLESLGGWLQR
                     RLTHASDSEERRRFVEGFVAASMLFCVGPLTILGSLSDGLGNGADQLLIKATLDGFAA
                     IAFASSFGWGVAASALVVAVVQGSLTLLGAAVGEVLPDPHLLAVTATGGLLLAGLGLR
                     LLQIKQIPVGDLLPALFVAPLLTAVVVGLR"
     gene            3447..4013
                     /locus_tag="VH419_08675"
     CDS             3447..4013
                     /locus_tag="VH419_08675"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY7043727.1"
                     /translation="MTRGPVVVRDAVPADAPALQEIWADFNVDTERGVRTECSCAETE
                     RAVLRLETDPSERLLVAILDEQPVGVAHLRRAPVSPIHEEDAVHVGYLHVLSDYRRRG
                     VGKQLLETAADWADEKDSKHIVAVVAATARVSNRFLARLGLSQVAIVRATTVAALRCK
                     LSGTASPKNVKTNVIATRRLMRRSRVSS"
     gene            complement(4176..4622)
                     /locus_tag="VH419_08680"
     CDS             complement(4176..4622)
                     /locus_tag="VH419_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020388828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HEY7043728.1"
                     /translation="MATPESASGAGPELADLLGEVTTIHDRMGIEITEAGPQRVVGTM
                     PVEGNRQPFGLLHGGASCVLAESLGSIGATMHGYPDKVAVGTDINATHHRAVSDGVVR
                     GVATPVHLGRTMACYEIVITDAADRRVCTARLTCQLVAHPPRRSAR"
     gene            4711..7434
                     /gene="polA"
                     /locus_tag="VH419_08685"
     CDS             4711..7434
                     /gene="polA"
                     /locus_tag="VH419_08685"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008362936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEY7043729.1"
                     /translation="MAETETLPAETAHQESEVSRPVLLLLDGHSLAYRAFFALPVENF
                     STTTGQPTNAVYGFTSMLINVLRDETPTHLAVAFDVSRQTFRTEEYAEYKANRSTSPT
                     EFSGQVSLIKEVLDALRISYLEKPGFEADDVIGTLATQAVAAGYDVLICSGDRDSFQL
                     VNDRTTVIYPMRGVSEMKRMTPAAVMERYGVPPERYAELAAIVGESSDNLPGVPGVGQ
                     GFAAKWLNQYDGLDGVIANADKITGKKGESLREHLGDVLRNRQLNALVCDLELPMRPA
                     DLTVQPWDRDEVHKIFDGLEFRVLRDRLFETLSADDPEIGPSTEINGRQLAAGELAGW
                     LAGHASGPAPTGVEVQGRWGAGTGQVTSVALADSTGTAAWIDAAELSPDDEVVLAGWL
                     ADPDRPKALHDAKGPMLALSARGWPMAGLAADTALSAYLSHPDQRSYDLADLTLRYLH
                     RELKSAASSTDQLSFDLDGDSALAQESMQKANAVVDLAAALETELATRGGSALLAEVE
                     LPLVDVLAGMEQVGVGIDVDHLTSLEAQFAGRVKQAADDAYAVIGKQINLGSPKQLQV
                     VLFDELGMPKTKRTKTGYTTDAEALQSLYDKTEHPFLAHMLEHRDASRLRQTVEGLLK
                     SVSDDGRIHTTFNQMIAATGRLSSTEPNLQNIPIRTEEGRRIREAFMVGSGFESLLTA
                     DYSQIEMRIMAHVSDDAGLIDAFHSGEDFHSVMAARVFQVDGAAVSVAQRSKIKAMNY
                     GLAYGLSAFGLSQQLKIETSEARALMDEYFERFGGVRDYLHGVVEEARRSGFTETILG
                     RRRYLPDLTSDNRQRREMAERMALNAPIQGSAADIIKVAMLKVDRALASDGMASRMLL
                     QVHDELVFEVAPGEREQLEALVRRQMAAAADLAVPLEVSVGLGRTWHEAGH"
     gene            7678..>8100
                     /locus_tag="VH419_08690"
     CDS             7678..>8100
                     /locus_tag="VH419_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043730.1"
                     /translation="MLKTLLAGVILALFGAATVALGDVLGLDLDHVALLGAALGAVVG
                     LVPDRAPLLRAFGFLVGMVIGWAVFGLRAGYLPDTSSGRAVATFIAIMLCVGVTVATL
                     RRIALWSTLVGVAGIVGAYESTYTNAPSQFIHTSGTAFT"
BASE COUNT         1378 a         2745 c         2718 g         1259 t
ORIGIN      
        1 gcagcatgag cgcgagcccc aggaagatga accgcatcaa actcgcctgc tgcggatcca
       61 tgatcgaggc cgggatcagc gggtcaggcg gttgcgtggc gtcgtcgaag aagttcacca
      121 ggaagctgat caggaaccag aagatcaccg ctccgaggat cggaccgacc acacgggcgg
      181 ctccgccgag caggaggacc gtccacgcgt agaacgtgat gtcggtcgcg aagttcgtcg
      241 gctgcaccga ctggtagaga ccggagacga tgccggcgaa gccaccgatg acaccaccga
      301 tcatcaggga ctgcatcttg taggcgtaga cgttcttgcc gaggctgcgt acggcgtcct
      361 cgtcctcccg aatccccttg aggacgcggc cccacgggct gcgcatgagc aggtagatcg
      421 ccacacagca gatcgcgacc agcgcccagc cgaccgtcat cacccagaag tcggccctgc
      481 tccacgagag cccgaggatg ttgacctctc cgttgtacgg gttgaggtcg tagaacgccg
      541 acgtgtactg ctgcagtccg tcctgccctc cgaagtactc acgcaggctc gaggctccga
      601 tgacgaagcg gatgatctcc gctgccgcga tcgtgacgat ggccaggtag tcggcgcgca
      661 gccgcagtgt cgggatgccg agcaacagcg ccagcacgat gcacagcccg ataccgacca
      721 ggcagccgaa ccagaagttt aagccccagg tggcgaccat gctcgcgacc ccgtagccgg
      781 ccaccgccat gaaggcggct tggccgaagt tgagcagccc ggtgtatccg aagtgcacgt
      841 tcaggccgac cgccgccaag cagatgatga tggccgacgg gccgagtccc tgctcgagcg
      901 ccagggtcac ttcgctgtga aagtccatgt tctccctcgc cctcagccga cccgctcagc
      961 ccggcccagc agcccctgcg gccggaacag cagtacgacg atcagcaccg ccaacgcgcc
     1021 ggcgaacttg agctccgacg ggatgatcag tgtcgacatc tcgatgaaca caccgacgat
     1081 cacgctgccg aggatggcgc cccagatggt gccgaggccg ccgaggacca cggctgcgaa
     1141 gaccaacagc aagatcttga acccgagctg atagtcgaag ccctgcgtca gtcccaacat
     1201 gaagccggac aggccggcca atgcgccgcc accgatccag acaacggaga tcacccggtt
     1261 gacgttgatc cccgacgacg acgccagggc ggggttgtcg gccactgccc gggtcgcctt
     1321 ccccatcctc gtacgctgca gcgcctgcgt cacggcgatg agcaccgcga ctgccatgac
     1381 cgtgacgatc agctccttgg gcgtgatgtc gaccgtgccg atcgaccacg gttccacggt
     1441 cgcgtactgc gtgtagttgt ggttgtccgc cccggcgatg tactggtaca cgctgcgcag
     1501 gaagatcgac aggccgatgc tgacgatcat catcgggatc aggccggtgc cgcgacgtcg
     1561 cagcgggcgc cacaagccgg cgtcctgcgc ccacccgaag acaccgctga cgatcgtcgc
     1621 gaggatgccc gcgaggatca ccggcagccc ccagccggcg tcgaaggcgt acgcgacgag
     1681 tgctccgaac gtgaccatct cgccgtgcgc gaagttcgtg aggccggtgg tcccgaagat
     1741 catcgagagc ccgagcgctg cgagcgccag gatcgagctg aagatcaggc cggtcacgag
     1801 gtcctggagt gccgtctcgt accacggtgt cgagggggtg aggtcgggcc cgatcgggaa
     1861 cgtgaagctg atgttcccct cgatgacgat ccgcttcgtg ttctcgacgt cttcggggtt
     1921 ggtgagcgac gacccctcgg gcaacgtgtc cgtgtcgatc ttcaccgtga tggtcttgcc
     1981 gaggttgtcg atcgccgcac ctggcagcgg cacgacgaag gtcccgttct tgccggactc
     2041 ggcctggccg atgatggagc cgtcggactg gtcgatgacg gtgatcgtca cgcccggcac
     2101 cggtgtgcac gggttggtgc gggtgtcgct gagctgcccg acgatctgga tggtgtcctc
     2161 tttggccggg atctgcacat gggggcacct gctcggagcc gtggcggctg cctggtcgct
     2221 gagaccggat cctgccgacg cctgtgcagg cgcggcaaac cacacgagcg atacgacggc
     2281 gatcactgcg gcgaagaccg tcgctgccag cacggtaagg cgcactcgca cggatgactc
     2341 ctgtcgcggt tcgcggtggt ctcgacgacc gggggtggcg ggactgtacc gctttcgccg
     2401 gagcatagtc acgcatcggt cgaagcgggc acaggccacg cctcgagcgc ctcgttgaaa
     2461 cccgatcgag acccgcttcc gggagctgtc gagtgcgtgg tcagcgcagg ccgacgacga
     2521 ccgcggtcag cagtggtgcc acgaacaggg cgggcaacag gtcaccgacc ggaatctgtt
     2581 tgatctgcag caatcgcaac ccgaggcccg cgagtaggag cccgccggtg gccgtgacgg
     2641 cgagcagatg cgggtccggc agaacctccc cgaccgcagc tccgagcagg gtcagcgatc
     2701 cctggacgac cgcgacgacg agtgcggatg ccgccacgcc ccacccgaag gacgaggcga
     2761 acgcgatcgc ggcgaaaccg tcgagggttg ccttgatcaa caactggtcg gcgccgtttc
     2821 ccaggccgtc ggacagggag ccgaggatcg tcagaggacc gacgcagaac agcatcgagg
     2881 ccgcgacgaa tccttcgacg aaacgccgtc gctcctccga atcgctggca tgcgtgagcc
     2941 gtcgctgcag ccagcctccg agcgactcca ggcggtactc gatctggacg agcgacccga
     3001 tgatgccgcc gatcaggagt gatccgagca cgatcagcac aggagcggag tcgccgacag
     3061 cgtctcgaag gtcagggtcg gtcacggcaa atgccgacaa ggccgccacc agcaaggtga
     3121 ccagccccag ggcgtcggtg acgacgtcgc gcgttcgctg cgagagacga tgtccgagga
     3181 gtacgccgat cgacgaaccg aggaggacgg tcgcgacgtt gacgacggtt ccgaagccgc
     3241 gaaacaccgc cagtcctcct caaggtgcag cgtgatcgag tcgctcgtcg aagcgttccg
     3301 gcctgtggac aatcgccgga cccggtggca ggaccggagc gtccgtcgta gtctggtcgc
     3361 cgcgtcggtc ccgcgtgagc ctatcggctc cttcgcagcg tggggtcgac tggccgctcg
     3421 ggggagtgac gagaaacgga ggagtcatga cccgaggtcc cgtcgtggtg cgtgacgccg
     3481 tccccgcgga tgcgccggca ttgcaggaga tctgggctga cttcaacgtc gacaccgaac
     3541 gcggcgtacg caccgagtgc tcgtgcgccg agacggagcg cgccgtgctt cggctcgaga
     3601 ccgatccgtc ggagcggttg ctcgtggcga ttctggacga gcagccggtc ggggtggcac
     3661 acctgcggcg cgcgccggtc tcaccgatcc acgaagagga cgccgtgcac gtcggctacc
     3721 tgcacgtgct cagcgactac cgacgccgcg gggtcgggaa gcagctgctc gagaccgcgg
     3781 cggactgggc cgacgagaag gactccaagc acatcgtggc cgtcgtggca gcgaccgctc
     3841 gcgtctcgaa ccgcttcctc gcgcgcctcg gcctcagtca ggtcgcgatc gtcagggcga
     3901 cgaccgtggc cgccctccgg tgcaagctgt ccggcaccgc gagcccgaag aacgtgaaga
     3961 ccaacgtcat cgccacccgc cgcctcatgc ggaggtcacg agtcagcagc tgactacacc
     4021 ccttccacgg gttgattgct tccggtcgat acccgccggc gccgccgccg tgtcttgcct
     4081 cttgcgcgcc tctgccaacg ccccttccgc ctctcggtga ggtggcaggt ttcgttgctt
     4141 tctggcggcg tgtcgggccg tgattgccac ctcgatcagc gggccgacct gcgcggcggg
     4201 tgagcgacga gctggcaggt gaggcgagcc gtgcagacac ggcggtccgc cgcatcggtg
     4261 atgacgatct cgtagcacgc catggtgcga cccaggtgca ccggtgtggc gacgcccctc
     4321 accacaccat cggagacggc gcggtgatgg gtcgcgttga tgtcagtgcc gacagccacc
     4381 ttgtccgggt agccgtgcat ggtggcgccg atggagccga ggctctccgc gaggacgcac
     4441 gatgcaccgc cgtgcagcag cccgaacggc tgccggttgc cctcgaccgg catggtgccg
     4501 accacacgtt gcggacctgc ctccgtgatc tcgatgccca tccgatcgtg gatcgtggtc
     4561 acttcgccga gaaggtcggc aagctccgga cccgcgccgg atgcgctttc tggcgttgcc
     4621 atgcgtcctc gatttctccc cacgtcgccg ggtccggacc gggaccgtac ggctcggttc
     4681 ggtgtcggag gtccagacta gggtgagcct gtggccgaga ccgagacgct ccccgccgag
     4741 acagcgcacc aagagtccga ggtcagccga cctgttctgc tgctcctcga cgggcactcg
     4801 ctcgcctacc gcgccttctt cgcccttccg gtggagaact tctcgaccac caccggacag
     4861 ccgacgaacg ccgtctacgg cttcacctcc atgctgatca acgtcctgcg cgacgagacg
     4921 cccacccacc tcgccgtggc gttcgacgtg tcgaggcaga cgttccgcac cgaggagtac
     4981 gccgagtaca aggccaaccg ctcgacctcg ccgactgagt tctccggcca ggtctcgctg
     5041 atcaaggaag tcctcgacgc gctacggatc tcctacctcg agaagccagg gttcgaggcg
     5101 gacgacgtga tcgggaccct ggcgacgcag gcggtcgccg ccggctacga cgtcctcatc
     5161 tgcagcggcg accgcgactc cttccagctc gtcaacgacc gcaccacggt gatctacccc
     5221 atgcgcggcg tgtccgagat gaagcggatg acgcctgcgg cggtcatgga gcggtacggc
     5281 gtccccccgg agcgctacgc ggagctggcc gccatcgtcg gggagtcgtc cgacaacctc
     5341 cccggcgtcc caggagtagg gcagggcttc gcggcgaagt ggctcaacca gtacgacggt
     5401 ctcgacggcg tcatcgcgaa cgccgacaag atcacgggca agaaggggga gtcgttgcgt
     5461 gagcacctgg gtgacgtgct gcgcaaccgc cagctcaacg ccttggtgtg cgatctcgag
     5521 ctcccgatgc ggccggccga cctgacggtc cagccctggg accgcgacga ggtgcacaag
     5581 atcttcgacg gtctggagtt ccgggtgctc cgtgaccggc tgttcgagac gctgtcggcc
     5641 gacgacccgg agatcgggcc gagcaccgag atcaacgggc ggcagctcgc cgccggtgag
     5701 ctggccggct ggttggccgg gcacgcgagc ggtccggcgc ctaccggtgt cgaggtgcag
     5761 ggccgatggg gcgcgggcac gggtcaggtc acgtctgtgg cactggccga ctcaaccggt
     5821 acggccgcct ggatcgacgc cgccgagctc agcccggatg acgaggtggt gctcgccggc
     5881 tggctggccg acccggaccg gccgaaggcg ctgcacgacg ccaaaggccc gatgcttgcg
     5941 ctgtcggcgc gtggatggcc gatggccggt ctggcggccg acaccgctct ctcggcgtac
     6001 ctctcccacc ccgaccagcg gtcctacgac cttgccgacc tgacgctgcg ctacctccac
     6061 cgtgagctca agagcgcggc ctcctccacc gaccagctgt cgttcgacct cgacggcgac
     6121 tcggcgctcg cccaggagtc gatgcagaag gcgaacgccg tcgtcgacct ggcggctgcg
     6181 ctggagaccg agctggccac tcgagggggc tcggcgctgc tggccgaggt ggagctgccg
     6241 ctcgtcgacg tgctggcggg gatggaacag gtcggcgtcg gcatcgacgt cgaccacctc
     6301 acgtcgctgg aggcacagtt cgccgggcgc gtgaaacagg cggccgacga cgcgtacgcc
     6361 gtgatcggca agcagatcaa cctcggctct cccaagcagc tccaggtggt gctgttcgac
     6421 gagctcggga tgccgaagac caaacgcacg aagaccggct acaccactga cgccgaggcg
     6481 ctgcagtcgt tgtacgacaa gaccgagcac ccgttcctcg cccacatgct cgagcaccgc
     6541 gacgcgagcc ggctgcggca gaccgtcgag ggcctgctca agtcggtgtc cgacgacggg
     6601 cgcatccaca cgacgttcaa ccagatgatc gccgccaccg gccggctgtc gtccaccgag
     6661 cccaacctgc agaacatccc gatccgcacc gaggagggtc ggcgcatccg cgaggcgttc
     6721 atggtcggct ccggcttcga gagcctgctc acggccgact acagccagat cgagatgcgc
     6781 atcatggcgc acgtctcaga cgacgccgga ctcatcgacg ccttccactc cggtgaagac
     6841 ttccactcgg tgatggcggc ccgggtcttc caggtggacg gcgccgctgt cagtgtcgcc
     6901 cagcgctcca agatcaaggc gatgaactac ggcctggcgt acggcctgtc ggcgttcggg
     6961 ctcagccagc agctgaagat cgagaccagc gaggctcgcg cgctgatgga cgagtacttc
     7021 gagcgcttcg gcggcgtccg tgactacctg cacggggtcg tcgaggaggc ccgccgcagt
     7081 ggcttcaccg agacgatcct tggccgccgc cgctatctgc cggacctcac cagcgacaac
     7141 cgtcagcgac gcgagatggc agagcgaatg gcgttgaacg cgccgatcca gggcagtgct
     7201 gccgacatca tcaaggtggc gatgctgaag gtcgaccgtg ccctggcgag cgacgggatg
     7261 gcgtcccgga tgctcttgca ggtccacgac gagctcgtgt tcgaggtcgc ccccggtgag
     7321 cgcgagcagc tcgaggcgct cgtacgccgg cagatggctg cggccgctga cctggctgtg
     7381 ccgctcgagg tgtcagtcgg ccttggacgc acctggcatg aggccggcca ctagctcgac
     7441 gccgaccgcc accaggtgtc cccgcgggcg tcccacgcgg atccctccaa ctcgggcata
     7501 ctgccggcgc gactctacct gcgggtaact ttcgtcacaa cggcagcctg ccgtggtgac
     7561 gcttgacccg cacacatgtc acactcctcc gcgagggagc ggcggacgac gcaccaccac
     7621 gcggcatggt ggccatgccg acccacgact caccaggacc cgtccgccaa cacacacatg
     7681 ctgaagacgc tgcttgccgg tgtcatcctg gcgctgttcg gagcggccac ggtggcgctg
     7741 ggcgatgttc tcggcctcga cctcgaccac gtggcgctgc tcggcgcggc gctgggtgcg
     7801 gtcgtcggcc tcgtcccgga tcgggctccg ctcctgcggg cctttggctt cctggtcggc
     7861 atggtgatcg gctgggccgt gttcggcctg cgcgccggtt acctgcccga cacctcctca
     7921 gggcgcgcgg tggcgacgtt catcgcgatc atgctctgtg tcggtgtcac ggtcgcgacg
     7981 ctgcggcgta ttgcgctgtg gtcgaccttg gtcggtgtcg cgggcatcgt cggggcgtac
     8041 gagtcgacct acaccaacgc accgtcacag ttcatccaca cgtccggtac ggcgttcacg
//
LOCUS       DATBFZ010000283        15136 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_3147711, whole genome shotgun sequence.
ACCESSION   DATBFZ010000283 DATBFZ010000000
VERSION     DATBFZ010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 15136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15136
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3147711"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..953)
                     /locus_tag="VH419_08695"
     CDS             complement(87..953)
                     /locus_tag="VH419_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020580207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEY7043731.1"
                     /translation="MGRAPAEDTQLRFEGMPKRLLQAAPSRLLTYLDCPRRYRFTYLD
                     RPAPSKGSPWAHNSLGAAVHTALANWWKLPVPQRTVPRAGALLDEAWLPDGFRDDSQR
                     QAARVRARAMVEGYVDGLDPLDEPVGVERTVALKTDHAALFGRIDRIDERVGEGLVVV
                     DYKTGRHVLTVDDARTSLALAVYAVGAARTLRRPCHRVELHHLPTGEVVSWVHTEESL
                     ARHVRRADSIAAEIGVLDARFRAGVDAAEADAMYPTRVSSLCGWCDFNRCCREGSSAV
                     APRDPWAGLDDP"
     gene            complement(970..1848)
                     /locus_tag="VH419_08700"
     CDS             complement(970..1848)
                     /locus_tag="VH419_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008364169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEY7043732.1"
                     /translation="MQIDLHTHSSASDGTQPPAELVAEAKQVGLDVIGLTDHDSAAGW
                     AEGAQAARRLGVTLVPGMELSTRLDGAGVHLLAYLPDPTYPPLVDELTKILAGRTGRL
                     AAMVGRLRAAGIDITEDEVLAQVGDAPAIGRPHIADVLVRKGVVADRSAAFRDWLNWG
                     RPAYVSRYASPTREMIAIVNAAGGAAVLAHPWGRGSRQVLDRRTMAVLATDGLAGIEV
                     DHQDHDESDRAVLRQMGRDLDLVVTGSSDYHGAGKVDHDLGCNVTSEQELDRLLTRAA
                     AHAARSGRDVPAVVGA"
     gene            complement(1874..3376)
                     /locus_tag="VH419_08705"
     CDS             complement(1874..3376)
                     /locus_tag="VH419_08705"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY7043733.1"
                     /translation="MPTPSVSTPEQVERRRWIALAVCCSALFMTLLDLSITNVALPSI
                     GDATGAGPAELQWVVSGYTLAFGLVPVLGGRLGDDHGRRLMFIVGVAGFVVTSALSGL
                     SPNSGLLIVARVLQGLSGGLINPQVSGLVQQMFRGRDRGRAFGVIGTVVALGTALGPL
                     VGGALIALGGPDFGWRLVFFVNIPIGLAVIVLARRWLPDPPATGQQSLDIIGALVLGL
                     ATFCILFSAVEYDAVRDDRLFLLGIPALVLLLVFVRREARLTRERRDPLVDLRLFRQP
                     SFTAGVAVALVFFCGMTGLPLVLALYYQQGLGYSALESGLGITAFAVGSAIAAPAAGR
                     VVTRVGRPLVVGACLTFGVGALALDVVARHQPGGNAAVAFAVPLFVLGLGAGGLITPN
                     QTLTLVDVDPVVGSTAGGVLQTSQRIGAAIGQAVIGATFFAALPAGAIDLVGTDRTDA
                     YGHALSQAVVVTLLFVGAALLVGLTDLFLTRRRRLIARNGAHRDAPVAQP"
     gene            complement(3437..3979)
                     /locus_tag="VH419_08710"
     CDS             complement(3437..3979)
                     /locus_tag="VH419_08710"
                     /inference="COORDINATES: protein motif:HMM:NF042893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6758 family protein"
                     /protein_id="HEY7043734.1"
                     /translation="MQARLTVVDPFAAVIGGEINTLSMPLWTPWPVPFGWTFGGFAHA
                     EASESGGAASVASWSGTDPFGDPAEIVFVCEEAGAGVGSHFADLPMSYPGHGVGEGPP
                     HAKFTLEGRPVPLWAVEAAPDRAVYAGEAAGRWLWVIVHPIESGAIMVQPIQLVDARA
                     LGAELTVLPVGELSPRLVVD"
     gene            4052..4453
                     /locus_tag="VH419_08715"
     CDS             4052..4453
                     /locus_tag="VH419_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012922944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEY7043735.1"
                     /translation="MRVPCVGVVVLDEAGRLLVVRRGHEPGRGRWSIPGGRVEPGESL
                     EAAAAREVLEETGLTVSIGSVVGRVELAGVGADVYDVTDFAGVVVDGVAAAGDDAADL
                     RWVSRSELDSLPTSAGLTSTLDSWHIWPSPR"
     gene            complement(4588..5163)
                     /locus_tag="VH419_08720"
     CDS             complement(4588..5163)
                     /locus_tag="VH419_08720"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012922950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY7043736.1"
                     /translation="MSEGEDEVWLVRHGETEWSKSGRHTGTTDLPLTDLGVAAARALH
                     DTLTGTPFDLVLCSPRLRARHTAELAGVTDVHIEDDLVEWDYGSYEGRTRIDIHGERP
                     GWSIWTDGAPDGETPAHIAHRVERVIGKCRANGGRVLLIAHGHVLRTLAARWIDQPVT
                     VGAHLPLDTAKVSVLSYDRGMPTLDRWNADT"
     gene            complement(5339..5866)
                     /locus_tag="VH419_08725"
     CDS             complement(5339..5866)
                     /locus_tag="VH419_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEY7043737.1"
                     /translation="MQFGRSYEEFEVGATYKHWPGKTVTEYDDHLFCLLTMNHHPLHL
                     DAHYAEETTQFGRNVVVGNYVYSLLLGMSVPDISGRAIANLEVESLRHVAPTFHGDTI
                     YGETTVIDKWESTSKDDRGIVHVETTGYTQDGTVVCTFRRKVMVPKQNYLEARGGEQP
                     GRPVPRERDERGPAR"
     gene            complement(6140..6538)
                     /locus_tag="VH419_08730"
     CDS             complement(6140..6538)
                     /locus_tag="VH419_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017935206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3037 domain-containing protein"
                     /protein_id="HEY7043738.1"
                     /translation="MRTSDLIGYQYVVLRCVPRVDREEFVNVAVVLYSQRADYLRSRS
                     DVDESRLTAIAPGFDVGAVHEALEAVEDVCEGRPSAGLPGQATRSARFGFLTAPRSTV
                     VQPGPIHGGLTADPDAELSSLVARLVTAPV"
     gene            complement(6535..7302)
                     /locus_tag="VH419_08735"
     CDS             complement(6535..7302)
                     /locus_tag="VH419_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA family kinase"
                     /protein_id="HEY7043739.1"
                     /translation="MIRQVQALRYVTPLREGGSLPGIVEADDLGTYVVKFRGAGQGLR
                     VLVAEVIVGELARRLGIRVPDLVAVDLDEQIAKYESDGEVQDLLSASIGLNLGSDFLP
                     GAFGYDAHCSPDPSEAAAILWLDAYVANVDRSWRNPNLLVWHRRVWAIDHGATLYFHH
                     GWTDGLTPPERFARQRYDARDHVLGGYAGGVPAAHDRLVAAVDERLISEVVALVPDAW
                     VDPVPGADRETVRAAYVDFLLARLAHAADWLPVERAA"
     gene            complement(7299..8432)
                     /locus_tag="VH419_08740"
     CDS             complement(7299..8432)
                     /locus_tag="VH419_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007079622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HEY7043740.1"
                     /translation="MAHVSEGAGTTDRAWVHEAIRKVEADNNRSADTHLHVFPLPDRW
                     GVDLYLKDESVHPTGSLKHRLARSLFLYALCNGWIGPGTTIIEASSGSTAVSEAYFAR
                     LLDLPFVAVMPRSTSAEKIALIEFQGGRCHLVDDPGSIYAESHRLAEECDGHFMDQFT
                     YAERATDWRGNNNIAESIFGQLAHERHPIPSWIVVGAGTGGTSATIGRYVRYRRLSTR
                     MLVVDPENSAFYDGWSRADPEHTTGRASRIEGIGRPRVEPSFLPTVIDAMQRVPDAAS
                     IAAMRWCTEITGRSVGGSTGTALWGAVQLIATMREHGENGSVVTLICDGGERYRDTYY
                     DDTWVTSHGLELEPYTKALDAFLDTGRLELPGMQPPGHLGVPS"
     gene            8538..9734
                     /locus_tag="VH419_08745"
     CDS             8538..9734
                     /locus_tag="VH419_08745"
                     /inference="COORDINATES: protein motif:HMM:NF018479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HEY7043741.1"
                     /translation="MSAASYLSYVRGMRHRDPDETHRASTPLELLFDLTFVVAVAHVA
                     AELAHALVTDDIGHGVVAYVMLFFAIWWAWMNFTWFASAYDCDDALYRIMTLVQMAGV
                     LVLAAGVDAAFEDGNFTTVVIGYIIMRVAMVGQWLRAGLGDVDGRATAFRYAGGTLVV
                     QIGWTARLWLPDRLGMASFLVLVLAELAVPPWAERRRRTAWHPEHIAERYGLFTIIVL
                     GESVLASSNAVSADFADGGLTLDLVLLGAGGLVLLFAMWWVYFLHDTGEALRSRPHLD
                     FLWGYSHYFIFASAAAVGAGLEAAIAHDVHPAAESASQLTLGLALAIAAGGYLLLVAL
                     IHARLSPSHRPTFVQAAGAAGLLGAIALTVAPSNLPLAVVLDAAVAMGLVAVGTLSTT
                     EVGKEA"
     gene            9772..10650
                     /locus_tag="VH419_08750"
     CDS             9772..10650
                     /locus_tag="VH419_08750"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY7043742.1"
                     /translation="MERPVRRTAIIGVLAAGTLAISGCAGSESGSQPDPHTPSRESPS
                     ASAEVIDGPVDIGGRSLYLQCDGDGPSTVILEAGLTGDHRTWDALFSDLTSLARVCAY
                     DRANVGASDPARTPRTADEMVADLDALLTAAQQPPPYVLVGFSFGGLVSQLYARTHPN
                     DVDGLVLVESNHPDEAEQFEAELTPKQIAADHREVNANEEGVDVYRSFAETQQAAAMP
                     DVPLVVVTAGVPEDWPPGWDSRVFDRLRAAQQADLASLVPNGTQVIAKQSSHDVPVQQ
                     PEIIVQAVKTVLAQAD"
     gene            11162..13282
                     /locus_tag="VH419_08755"
     CDS             11162..13282
                     /locus_tag="VH419_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020579005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY7043743.1"
                     /translation="MTTAPTTALTLTPERRAIADRVIAAMAGDDAKLRSDQETAVAAL
                     CEPSARVLVVQATGWGKSAVYWAATAVRRAEGHGTTLVVSPLLALMRDQVAAAARAGL
                     TAATLNSSNVTEWTAIEEQLLAGSLDVLLVSPERLANPRFANRVLDALVGRLGLLVVD
                     EAHAISDWGHDFRPDYRRVADLLRGLHPTTPVLATTATANARVTDDVAAQLGETTLVC
                     RGRLARDSLHLAVLGELSPLERYAWVVDHLPRLPGSGIVYALTVADAERLATVLQAVH
                     GASLRVAAYTGGLEPETRERLEDGLRSNQLKALVATSALGMGYDKPDLGFVVHVGSPP
                     SPVSYYQQVGRAGRGIDEAQVILLPSTADAGVWDYFATATIPDADQVNRLISAWGREG
                     DETTSIPALEAISGVRRGRVELLLKQLAVDCVVERVEDGWRLTGRAWVYDAEHYDGII
                     ATRRREAEIMRAYSRGESCLMQLLQESLDDPTAQPCGRCTVCRGGLPDGLAGVPARAT
                     VTDVSRRLRGESHTLESRKLWPGGEFGSRGRIPDEVMADEGRVLIHANAPEWREFLAG
                     GVGPGTTATSELLDGCVALMSRWRSQWAERPEVVVGLAAAGAEELVEQVCAHIASVGR
                     LDATLIPSDGSAPDGLASASEAAWWRDRISVGPETRTSVAGRPVLLVVDASSSMWPVT
                     IAAAALREAGAKRVLPLVVHRRVG"
     gene            complement(13325..13867)
                     /locus_tag="VH419_08760"
     CDS             complement(13325..13867)
                     /locus_tag="VH419_08760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043744.1"
                     /translation="MRVPSSVADAVDAAHQLGRYVLLVRSVVGGAVVACWLVGSLIGG
                     FDPVTTIVLLTAATAATLRPDSPAPLVLIVLMAGFWMVEVRPMSVGWSIVLACCVLLV
                     HVSTSRAATLVEHSPYDRGVTRRWLAQTGVVAATTAVVWCVVLVLHDSPLPGGLLASA
                     AAFVAVAATLLALRAVTVDR"
     gene            complement(13864..>15136)
                     /locus_tag="VH419_08765"
     CDS             complement(13864..>15136)
                     /locus_tag="VH419_08765"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEY7043745.1"
                     /translation="LLGGVGVLVAVVFHRPDLLVASTPLVVVAVWGAGTRPGRPAQVT
                     GRLGRTTIREGQATALRVTVEAPGGAQEFWLGCTVSPWVRTRPAYGIAAGLVPRGESS
                     VNVELLLRMTRWGHHAIGPTTVAGLSAWGAYQWGPQILDGRDVEVLPSVPVFDTVAPM
                     PHPEGLVGIDRSARPGDGSEFATIRQFQLGDRLRRIHWPSSLRSNSLNVTATYADHDS
                     QVMVIVDASNDVGVSTGVDGSASTLDSTVRAAAALCEHYVRRGDRVGLLVTGAADRIL
                     VRPTTGRPHLRRMLSSLARVSAGGSLSAAREPRLHVHPGALVLMCSPLISSVPMERAL
                     GLARRGLIIVVIDTLPEDLDVLSDLETAGSREQQLTELAWRVRMLERDTDVRRLVQAG
                     VPVVPWRGPGSLDQVLRDVSRRARAPRLAPR"
BASE COUNT         2303 a         5290 c         5294 g         2249 t
ORIGIN      
        1 gaacagtgcg gcatggtcgg tcttgagcgc gaccgtgcgt tcgacacgcc ggcaggtcgc
       61 aacgattcct gccacctcgg cgaaggtcag gggtcgtcga ggccggccca ggggtcgcgc
      121 ggcgcgacgg cggacgagcc ctcccggcag caccggttga agtcgcacca accgcacagg
      181 ctggagacgc gggtcggata catcgcgtcg gcctcggcag cgtccacacc ggcacggaac
      241 cgcgcatcga gtacgccgat ctcggcggcg atcgagtccg cccggcggac gtgccgggcg
      301 aggctctcct cggtgtggac ccagctcacg acctccccgg tcggtaggtg gtgcagctcg
      361 acccggtgac acgggcggcg cagcgtacgg gctgcgccga ccgcgtacac cgccaacgcg
      421 agcgaagttc gcgcgtcgtc gaccgtgagc acatgccgac cggtcttgta gtcgaccacg
      481 acgagccctt caccgacgcg ttcgtcgatc cggtcgatcc gtccgaacag tgcggcatgg
      541 tcggtcttga gcgcgaccgt gcgttcgaca ccgaccggct cgtcgagcgg gtccagcccg
      601 tcgacgtacc cctcgaccat cgcccgggcg cggaccctcg ccgcctgccg ttgcgaatcg
      661 tcgcggaagc cgtccggcag ccacgcctcg tcgaggaggg cgccggcccg gggcacggtg
      721 cgttgcggga cgggcagctt ccaccagttg gccagtgccg tgtgcaccgc tgcgcccagc
      781 gagttgtgcg cccacggcga tcccttcgac ggcgcgggcc ggtccaggta ggtgaagcgg
      841 tagcgccgtg gacagtcgag ataggtgagc agccgagacg gggctgcctg gagcagccgc
      901 ttcggcatcc cttcgaaccg cagctgggtg tcctccgccg gcgccctgcc catcgtgtcc
      961 atctgcgcgt cacgctccga cgaccgcggg cacgtctcgg cccgatcgcg cggcgtgcgc
     1021 ggcagcccgg gtgagcagcc ggtcgagctc ctgctcactc gtcacgttgc agcccaggtc
     1081 gtgatcgacc ttgcctgcac cgtggtagtc actcgagccg gtgaccacca ggtcgaggtc
     1141 gcgccccatc tgccgcagca cggcgcggtc ggactcgtcg tggtcctggt ggtccacctc
     1201 gatcccggcc agcccgtccg tggccagcac ggccatcgtg cggcggtcga gcacctgccg
     1261 actgcctcgg ccccagggat gcgccagcac ggccgctccc ccggcggcgt tgacgatcgc
     1321 gatcatctcc cgagtcgggg aagcgtagcg ggagacgtac gccggtcggc cccagttgag
     1381 ccagtcgcgg aaggccgcac tccggtcggc gaccacaccc ttccggacga ggacgtcggc
     1441 aatgtgcggt cgaccgatcg cgggcgcatc gcccacctgg gcgagcacct cgtcctcggt
     1501 gatgtcgatg ccggcggcac gcaaccgtcc gaccatggcg gcgagccgtc cggtgcgccc
     1561 ggccaggatc ttcgtcagct cgtccaccag cggtgggtac gtcggatcgg gcagatacgc
     1621 cagcaggtgc acgccggccc catcgagcct ggtggacagc tccatccccg gcacgagcgt
     1681 cacgcccaac cgccgggcgg cctgcgcgcc ttccgcccag ccggcggcgg agtcgtggtc
     1741 ggtcagaccg atcacgtcca gcccgacctg cttcgcctca gccacgagct cggccggcgg
     1801 ctgcgtgcca tcggacgcgc tggagtgggt gtggaggtcg atctgcaccc cgccagcgta
     1861 ggcgggacaa ccgtcagggt tgggcgaccg gcgcgtccct gtgtgcaccg ttcctcgcga
     1921 tgagccggcg acgtcgcgtc aggaacaggt cggtgaggcc gacgagcagc gcagcgccga
     1981 cgaacaacaa cgtgacgacg accgcctggc tcaacgcgtg accgtacgcg tcggtccggt
     2041 cggtaccgac caggtcgatg gcgcccgcag gaagcgcggc gaagaacgtc gctccgatca
     2101 cggcctggcc gatggcggcc ccgatccgtt gggacgtctg cagtacgccg cccgcggtgc
     2161 tgccgacaac cgggtcgacg tcgacgagcg tcagggtctg gttcggcgtg atgagcccgc
     2221 cggcaccgag accgaggacg aacaggggta cggcgaacgc gacggcagcg ttgccgccgg
     2281 gctggtggcg cgcgacgacg tccagcgcga gcgcaccgac tccgaacgtc aggcaggcgc
     2341 cgacgacgag cgggcgaccg acgcgcgtca cgactcggcc ggccgccggt gccgcgatcg
     2401 ccgagccgac cgcgaaggcg gtgatgccga gaccggactc gagggcggag tacccgagcc
     2461 cctgctggta gtacagcgcg agcacgagtg ggagaccggt catcccgcag aagaagacca
     2521 gcgccacggc gacgccggcc gtgaacgacg gctggcggaa cagccggaga tccacgagcg
     2581 ggtcacgtcg ctcccgcgtc agtcgggcct cgcggcggac gaacaccagc agcaggacga
     2641 gcgcgggtat gccgaggagg aacagccggt cgtcgcgcac ggcgtcgtac tcgaccgccg
     2701 agaacaaaat gcagaacgtc gcgagcccga gcaccagcgc tccgatgatg tccaggctct
     2761 gttgcccggt cgccggcggg tcaggcagcc accgccgggc cagcacgatc acagccagac
     2821 cgatcgggat gttgacgaag aacacgagcc gccatccgaa gtcgggaccg ccgagcgcga
     2881 tgagcgcacc gcccaccaac ggtccgagcg ccgtgccgag agccacgacg gtgccgatga
     2941 cgccgaaggc ccgccctcgg tcacggccac ggaacatctg ctggacgagc ccggacacct
     3001 gcgggttgat gaggccaccg gacaggccct gcagcacgcg ggcgacgatc agcagtccac
     3061 tgttcggtga cagtcccgac agcgcactgg tcaccacgaa gcccgcgacc ccgacgatga
     3121 acatcagccg gcggccgtgg tcgtcgccga ggcgtccacc gagcaccggg acgagtccga
     3181 acgccagcgt gtaaccgctg accacccatt gcagctctgc tggtccggcg cccgtcgcat
     3241 cgccgatcga gggcagcgcg acgttggtga tgctgaggtc gagcagcgtc atgaacagcg
     3301 cgctgcagca caccgccaac gcgatccacc ggcgccgctc cacctgttcg ggagtcgaaa
     3361 ctgatggcgt cggcatcacc ctcgcaggct acgtccgggc gattgtcggc gccgcgcgaa
     3421 tatcggccag atcacatcag tcgacgacga gccggggcga cagctcgccg accggcagca
     3481 ccgtgagctc ggcgcccagc gcgcgtgcgt cgacgagctg gatgggctgc accatgatgg
     3541 cgccggactc gatcgggtgc acgatcaccc agagccatcg cccggcggcc tcaccggcgt
     3601 acaccgcacg gtccggcgca gcctcgaccg cccacaacgg tacggggcgg ccctcgaggg
     3661 tgaacttcgc atgcggcggt ccctcaccga ctccgtggcc gggatagctc atcggcaggt
     3721 cggcgaagtg gctgccgaca ccggcaccgg cttcctcgca gacgaacacg atctcggcgg
     3781 ggtcgccgaa cgggtccgtg ccgctccagc tcgccaccga ggcggcaccg cccgactcgc
     3841 tcgcctcggc gtgcgcgaag ccgccgaacg tccagccgaa cggcaccggc cacggggtcc
     3901 acagcggcat cgacagtgtg ttgatctcgc caccgatcac cgcggcgaac gggtcgacga
     3961 ccgtgagccg ggcttgcatg ggtcaacgtt ggtggggact gcgggtgacg gtcaaggtcg
     4021 gcgcacggtc ttcacggcgt acggtcacgt catgcgggtg ccgtgcgtag gggtcgtcgt
     4081 gctcgacgag gcgggtcggc ttctggtggt acgccgaggc cacgagccgg gccgcggccg
     4141 gtggtcgatc cccggcggtc gtgtggagcc gggcgagtcg ctggaagccg cggccgcgcg
     4201 cgaggtgttg gaggagacgg ggttgaccgt gtcgatcgga tcggtcgtcg gccgcgtcga
     4261 actcgccggt gtcggtgccg acgtgtacga cgtgacggac ttcgccggcg tcgtggtcga
     4321 cggtgtggcg gccgccgggg acgacgcggc cgacctgcgc tgggtcagcc ggtccgagct
     4381 cgactcgttg ccgacgtccg ccggcctgac ctcaaccctc gactcctggc acatctggcc
     4441 ctccccgcgt tgagtgctgg cttccgggct gttgagtgct gggtttccgg tggtcgagtg
     4501 ctgggtttcc tgtcgtcgag tgccggcttc cggacggtcg aaccgtggct tggcttcggt
     4561 gagctgtcct ctgcggccga tgcagcatca ggtgtccgcg ttccaccggt cgagggtcgg
     4621 cattccgcgg tcgtagctca gcacgctgac cttcgcggtg tcgagcggta gatgggcgcc
     4681 gaccgtgacc ggctggtcga tccagcgagc ggccaacgta cggagcacat gtccatgcgc
     4741 gatcagcagg acgcgaccgc cgttggcgcg gcacttgccg atgacccgct cgacacgatg
     4801 ggcgatatgt gccggggtct cgccgtcggg cgcgccgtcg gtccagatcg accatcccgg
     4861 acgctcccca tggatgtcga tccgcgtgcg tccctcgtag ctcccgtagt cccactcgac
     4921 gaggtcgtcc tcgatgtgca cgtctgtgac ccccgccagc tcggccgtgt ggcgggcgcg
     4981 tagccgcggg ctgcagagca cgaggtcgaa cggcgtaccg gtcaacgtgt cgtgcagcgc
     5041 ccgcgccgct gcgacaccga ggtccgtcag cggcaggtcg gtcgtgccgg tatgacggcc
     5101 tgacttgctc cactcggtct cgccatgacg gaccagccag acctcgtcct cgccctcact
     5161 cacctgaccg actctaggag ggtgctgaag aatccgctcg tcgcgagcgg ggtcgaggtg
     5221 gatgagccgc aaggcggagg agggaggcgg ggccaagccc ccgttgaccg acgacaacgc
     5281 cgcggatcgc cggctcgggc ccgcgcagca gagcatgatt cttcagcagt ctcctaggct
     5341 aacgcgctgg accgcgctcg tctcgctccc gcggcactgg ccgcccaggc tgctcgccgc
     5401 cacgcgcttc gaggtagttc tgcttgggca ccatgacctt gcgccggaag gtgcacacga
     5461 ccgtgccgtc ctgcgtgtaa ccggtcgtct ccacgtgcac gatcccccgg tcgtccttgc
     5521 tcgtgctctc ccacttgtcg atcacggtcg tctcgccgta gatcgtgtcg ccgtggaacg
     5581 tcggcgccac gtgccgcagc gactccacct cgaggttggc gatcgcccgg ccggagatgt
     5641 cgggcacgct catcccgagc agcagtgagt agacgtagtt gccgacgacg acgttccggc
     5701 cgaactgcgt cgtctcctcg gcgtagtgcg cgtccaagtg gagcggatgg tggttcatcg
     5761 tgagcaggca gaagagatgg tcgtcgtact ccgtcacggt cttgcctggc cagtgcttgt
     5821 acgtcgctcc gacctcgaac tcctcgtagc tgcggccgaa ctgcatgccc atctcctcac
     5881 ggcgctcgct gatcactctg ctggccgtcc atcgtggcag acacctcggc ggcgggcggc
     5941 ggggcgaccc cggcggcgtc cagtgtcccg cgtcagaagt ctttttcacg ctgcgccggt
     6001 ccaggcggcg cctcgcggcg ttgtcgtcgg tcgacgatgc tgcgcatcgc ctccctcctc
     6061 cgccttgcga tgcatccacc tgtcctcgcc gcacctgtga aggaacctcc gacgccggac
     6121 actagcctga cgcgagacat cagaccggtg ccgtgacgag ccgggccacc agtgaggaca
     6181 gctcggcgtc ggggtccgcg gtgagcccgc cgtggatggg accgggctgc acgacggtac
     6241 tccgcggcgc ggtcaggaag ccgaacctcg cagagcgggt cgcctgcccc ggaagcccgg
     6301 cggacggacg tccctcacag acgtcctcga ccgcctcgag ggcttcgtgc acggcgccca
     6361 cgtcgaagcc gggcgcgatg gcggtgagcc ggctctcgtc gacgtccgac cgtgagcgga
     6421 ggtagtcggc ccgctggctg tacagcacga cggcgacgtt gacgaactcc tcacgatcca
     6481 cgcggggaac gcagcgcagc acgacgtact ggtagccgat caggtccgac gtcctcatgc
     6541 cgcccgctcc accgggagcc agtcggccgc gtgtgccagc ctggccagca ggaagtcgac
     6601 gtacgccgcc cgcacggtct cgcggtccgc gcccgggacg ggatccaccc aggcgtccgg
     6661 tacgagtgcg acgacctcgg agatcagccg ctcgtccacg gctgccacca gtcggtcgtg
     6721 cgcagcaggc acaccgccgg catagccacc gaggacgtgg tcgcgcgcgt cgtaacgctg
     6781 tcgggcgaag cgttctggcg gggtcaggcc gtccgtccag ccgtgatgga agtacagcgt
     6841 ggcgccgtgg tcgatggccc acacccgacg gtgccagacg agcaggttgg ggttgcgcca
     6901 actgcggtcg acgttggcga cgtacgcgtc gagccagaga atggcggcgg cctcggacgg
     6961 atccggtgag cagtgcgcgt cgtagccgaa cgcgccgggc aggaagtcgc tgccgaggtt
     7021 gagcccgatc gaggcagaca gcaggtcttg cacctccccg tcggactcgt acttggcgat
     7081 ctgctcgtcg agatcgacgg cgacgaggtc ggggacgcgg attccgagcc gccgcgccag
     7141 ctcgccgacg atcacctcgg ccaccagcac gcgcagaccc tggccggccc cgcggaactt
     7201 gacgacgtag gtgccgaggt cgtcggcctc cacgatgccg ggcagcgaac cgccctcccg
     7261 cagaggtgtc acgtaccgca gcgcctggac ctggcggatc acgacggcac cccgaggtgc
     7321 ccgggtggct gcatcccggg gagctcgagg cgaccggtgt cgaggaaggc gtcgagtgcc
     7381 ttcgtgtacg gctccagctc gagaccatgc gaggtcaccc aggtgtcgtc gtagtacgtg
     7441 tcgcggtagc gctcgccgcc gtcgcagatc agcgtcacga cactgccgtt ctcgccgtgc
     7501 tcacgcatcg tcgcgatgag ctggacagct ccccacagcg ccgtcccggt ggatccgccg
     7561 accgaccggc cggtgatctc agtgcaccaa cgcatggcgg cgatggacgc ggcgtccggg
     7621 acgcgctgca tcgcgtcgat gacggtcggc aggaacgacg gctcgaccct cggccgaccg
     7681 atgccctcga tgcgggaggc ccggccggtt gtgtgctcag gatctgcgcg ggaccagccg
     7741 tcgtagaagg cggagttctc cgggtccacg acgagcatcc gcgtcgagag ccggcggtag
     7801 cggacgtacc tgccgatcgt cgcggacgtc ccaccggtgc ccgcacccac cacgatccat
     7861 gaggggatcg ggtgccgttc gtgcgccagc tggccgaaga tcgactcggc gatgttgttg
     7921 ttgccgcgcc agtcggtggc ccgctcggca taggtgaact ggtccatgaa gtgtccgtcg
     7981 cactcctcgg cgagcctgtg tgactcggcg tagatcgacc cggggtcgtc gaccaggtgg
     8041 cagcggccgc cctggaactc gatcaacgcg atcttctccg cggacgtgct gcgcggcatg
     8101 accgcgacga acggcaggtc gagcagccgg gcgaagtagg cctccgacac ggccgtggat
     8161 cccgagctcg cctcgatgat ggtcgtgccc ggtccgatcc agccgttgca gagcgcgtag
     8221 aggaacagtg acctggccag ccggtgcttg agactgccgg tcggatggac cgactcgtcc
     8281 ttgaggtaca ggtcgacgcc ccagcggtcg ggcagcggga acacgtgcag atgcgtgtcg
     8341 gcactgcggt tgttgtctgc ctcgaccttc cggatcgcct cgtgcaccca cgcccggtcc
     8401 gtcgtgccag ccccttcgct cacgtgggcc atcctccgcc gaggtggcaa gttcggtcca
     8461 tttctgtcgg cgtgtcgaca cgccatcgcc acctcggcgc ccgtggctcg aagggtcgct
     8521 gggctacgtt gtcgcgggtg agcgcggcgt cgtacctgtc ctacgtccgt gggatgcgtc
     8581 accgggatcc cgacgagacg caccgagcct ccaccccgct cgagctgttg ttcgacctga
     8641 cgttcgtcgt cgccgtcgca cacgttgccg ccgagctcgc acatgcactg gtgaccgatg
     8701 acatcggcca cggtgtcgtc gcctacgtga tgctgttctt cgccatctgg tgggcgtgga
     8761 tgaacttcac ctggttcgcc tcggcgtacg actgcgacga cgcgttgtac cggatcatga
     8821 cgctcgtcca gatggccggc gtactggtgc tcgcggccgg cgtcgacgcg gccttcgagg
     8881 acggcaactt caccacggtc gtcatcggct acatcatcat gcgtgtcgcg atggtggggc
     8941 agtggctgcg cgccgggctc ggcgacgtcg acggtcgcgc cacggcgttc cggtacgccg
     9001 gagggaccct ggtcgtgcag atcgggtgga cggcccggct gtggctcccc gaccggctcg
     9061 gcatggcttc cttcctggtg ctggtgctgg cggagctggc cgtgcctccg tgggcggaac
     9121 gcagacgaag gacggcctgg cacccggagc acatcgccga gcgctacggc ttgttcacca
     9181 tcatcgtgct gggagagtcc gtgctggcgt ccagcaacgc cgtctccgcg gacttcgccg
     9241 acggcggcct caccctcgac ctggtcctgc tcggtgcggg cggactggtc ttgctgttcg
     9301 cgatgtggtg ggtctacttc ctccacgaca ccggcgaggc gctgcggtcc cgaccgcacc
     9361 tggacttcct ctggggctac tcccactact tcatcttcgc ctcagcggcg gcggtgggtg
     9421 ccgggctgga agccgcgatc gcccacgacg tccatccagc cgccgaaagt gcgagccagc
     9481 tgacgctcgg cctggcgctc gccattgccg caggcgggta tctcctcctc gtcgcgctga
     9541 tccacgcacg gttgagcccg tcgcaccgac cgacgttcgt gcaagcggct ggtgccgccg
     9601 gactgctcgg ggcgatcgcg ctgaccgtgg ccccgagcaa cctaccgctc gctgtcgtcc
     9661 tggacgccgc ggtcgctatg ggcctcgtcg cggtgggcac cctgtccacc accgaggtcg
     9721 gcaaggaggc ttgacgctgg ccggacaatg gtcgcaggta tgacgggggt gatggagaga
     9781 cccgtgcggc gtacggccat catcggcgtc ctggcagccg gcaccctggc gatctccggt
     9841 tgcgccggct ccgagagcgg cagccagccc gacccgcaca caccttctcg tgagtctccc
     9901 agcgcgtccg ctgaggtcat cgacggaccg gtcgacatcg gcggtcgttc gctctacctg
     9961 caatgcgacg gcgacggccc gtcgaccgtg atcctcgaag cgggcctgac gggcgaccat
    10021 cgcacgtggg acgctctctt ctccgacctc accagccttg cgcgggtctg cgcatacgac
    10081 cgagccaacg tcggggcgag cgatccggcc aggacgccgc gcacggcaga tgagatggtc
    10141 gccgaccttg acgctctgct caccgcggca cagcagccac cgccgtacgt gttggtgggg
    10201 ttctcgttcg gtgggctggt gagccagctg tacgcccgaa cgcatccgaa cgacgtggac
    10261 ggtctggtgc tcgtcgagtc caaccatccg gacgaggccg agcagttcga agccgagctc
    10321 acaccgaagc agatcgcggc agaccatcgc gaggtcaacg ccaatgagga gggagttgac
    10381 gtctaccgga gcttcgccga gacccagcag gcggcggcga tgccggacgt cccgcttgtc
    10441 gtggtgacgg ccggcgttcc cgaggactgg ccaccgggct gggactcccg agtgttcgac
    10501 cggctgcgtg cagcgcagca agccgacctg gccagcctgg tgccgaacgg cacgcaagtc
    10561 attgcgaagc agagctcgca tgatgtaccg gtgcagcagc ccgagatcat cgtccaggcg
    10621 gtcaagacgg tgttggcgca agccgactga gcctcatcga gccctccggc agtcgagacc
    10681 tgcggcgact cacttcggcg gggactcctc gggtttctgt gcggcggaga cggtcgtgat
    10741 gggtgggcgt ggtcgccagg ctctctctcc tgccagtgat tgaggtgagt gggcaatgaa
    10801 gagcgggaaa cgttcgttct cgctgagccg gtcggccggc gttccgtccc gtttgcctgg
    10861 tgtggctggc gggtttcgga catccctggg tcgccgagcc gcggtttgca tggctcccca
    10921 ggcaacccca ccgctcccaa ggcagatctg cctggctacc cgtccgtgtg catggctacc
    10981 caggcaaacc caccaccgac acgtgacccg tgagatgcgc cggtagcagg cgcgggccat
    11041 aggaacccga ggagtagcct tcggggcgag ttcgccgtag cagtaggacg ccgtgtcggg
    11101 ttgatccaca gaaacgccgt cagccgcaca acgtcgccgc caccccgtag ggtcggggcc
    11161 gatgacgacg gcaccgacga cagccctcac cctgacgccc gagcgacgcg cgatcgccga
    11221 ccgggtgatc gccgcgatgg ccggcgacga tgcgaagctt cggtccgacc aggagaccgc
    11281 cgtggccgcg ctgtgcgagc cgagcgcccg cgtcctcgtc gtccaggcga ccggatgggg
    11341 caagtcggcg gtctactggg cggccaccgc ggtgcgtcgc gcggagggtc acggtacgac
    11401 gctcgtcgtc tcacccctcc tcgccctcat gcgcgaccag gtggctgccg cagcgcgcgc
    11461 cgggttgacg gcggccacgc tcaactcgtc caacgtgacg gagtggaccg ccatcgagga
    11521 acaactcctc gccggctcgc tcgacgtcct gctcgtgtca ccggagcggc tcgccaaccc
    11581 acggttcgcc aaccgcgtgc tcgacgcgct cgtcggccgc ctgggcctgc tcgtcgtcga
    11641 cgaagcacac gcgatttctg actggggtca cgacttccgg cccgactacc gccgcgtcgc
    11701 ggacctgctc cgcggcctgc acccgaccac accggtcttg gcgacgaccg cgacggccaa
    11761 cgcccgggtc accgacgacg tcgccgcaca gctcggggag acgacgctcg tctgccgtgg
    11821 tcgactggcc cgtgacagcc tgcacctcgc ggtgctcggc gagctgagtc cgctcgaacg
    11881 ctacgcctgg gtcgtcgacc acctgccgcg gctgccgggc agcggcatcg tctatgccct
    11941 gacggtcgcc gacgccgagc ggctcgccac ggtgctgcaa gcagtccacg gtgcgtcgtt
    12001 gcgggtagcg gcgtacaccg gcggactcga acccgagaca cgcgagcgac tcgaggacgg
    12061 tctgcggtcg aaccagctga aggcgctggt ggcgacctcg gctctcggca tgggatacga
    12121 caagcctgat ctcggcttcg tggtgcatgt ggggtcccca ccgagtccgg tgtcgtacta
    12181 ccagcaggtc gggcgcgccg gccgcggaat cgacgaggcc caggtgatcc tgctgccgtc
    12241 caccgcggac gccggcgtct gggactattt cgccacggcc accatccctg acgccgacca
    12301 ggtcaaccgg ctcatctcag cctggggccg agagggcgac gagaccacga gcatcccggc
    12361 gctcgaggcg atctccggag tacgccgcgg cagagtcgag ctcttgctca aacagctggc
    12421 ggtcgactgc gtggtcgagc gggtcgagga cggctggcgg ctgaccggcc gcgcatgggt
    12481 gtacgacgcc gagcactacg acgggatcat cgccacccga cgccgcgaag cggagatcat
    12541 gcgggcctac agccgcggtg agagctgtct catgcagctg cttcaggagt cgctcgacga
    12601 cccgacggcg cagccctgtg gccggtgcac cgtgtgccga ggcggtcttc ccgacggact
    12661 cgcgggcgtg cccgcacgtg cgacggtgac cgacgtcagt cggcggctcc gcggcgagag
    12721 ccatacgctc gaatcccgca agctgtggcc aggcggcgag ttcggcagcc gggggaggat
    12781 ccccgacgag gtcatggccg acgagggccg ggtcctcatc cacgccaacg ccccggagtg
    12841 gcgggagttc ctcgccggtg gtgtcggccc cggcacgacg gcgacgagcg agctgctgga
    12901 cggttgtgtg gcgctgatga gccgctggcg gagtcagtgg gcggagcgcc cggaggtggt
    12961 ggtcgggctg gccgcggccg gtgcggagga gctggtcgag caggtgtgcg cacacatcgc
    13021 ctcggtaggg cgcctcgacg ccacactcat cccatccgac ggatcggcgc cggacggtct
    13081 cgcgtccgcg tccgaggctg cctggtggag agaccggatc agcgtgggcc cggagacacg
    13141 cacgtcggtt gccgggcgtc cggtgctgct cgtcgtcgat gcatcgtcgt ccatgtggcc
    13201 ggtgacgatc gccgccgccg ccctccgtga ggcgggtgcc aaacgcgttc tgccgctcgt
    13261 ggtccacagg agagtcggct gagttccgta cgctcgcacc ccgtggccat cggcggggcc
    13321 ttcgtcagcg atcgacggtg acggcacgca gagccaggag cgttgccgcg accgcgacga
    13381 aggccgctgc ggaggccagc aggccgcctg gaagcgggga gtcgtggagg acgagcacca
    13441 cacaccacac cacggctgtc gtcgccgcga ccaccccggt ctgggccagc caacggcggg
    13501 tgaccccgcg gtcgtacggg ctgtgctcga ccagcgtggc ggcccgggac gtcgacacgt
    13561 gcacgagcag cacgcaacac gccagcacga tcgaccagcc aaccgacatg ggcctgacct
    13621 ccaccatcca gaagccggcc atcagcacga tgagcacgag cggtgccgga gagtcggggc
    13681 gcagcgtcgc cgctgtcgcg gcggtcagca agacgatggt cgtcacgggg tcgaagccgc
    13741 cgatcagcga gccgacgagc cagcacgcaa cgacagcgcc accgaccacg gagcgaacca
    13801 gcaggacgta tcgacccagc tggtgggccg cgtcgacggc gtccgcgacc gacgacggca
    13861 ctctcatcgg ggcgccaacc tgggtgcccg cgcacgccgt gacacgtcgc ggagcacctg
    13921 gtcgaggctg cccggtccgc gccacggtac gacggggacg ccagcttgga cgaggcggcg
    13981 tacgtcggtg tcgcgttcga gcatccgaac ccgccaggca agctccgtca gctgctgctc
    14041 cctcgacccg gccgtctcca ggtcggacag gacgtcgagg tcctccggca gcgtgtcgat
    14101 gacgacgatg atgagaccgc gtctggccag gccaagtgca cgttccatcg gaactgacga
    14161 gatcagcggc gaacacatca gcaccagcgc tcctgggtgg acgtgcaggc ggggctccct
    14221 cgcggccgac agcgagccac ccgcactcac ccgggccagc gagctgagca tccgacgcag
    14281 gtgcggccga ccggttgtgg ggcggaccag aatcctgtcc gcggcgccgg tgacaaggag
    14341 gcctacccga tcgccgcgcc gcacgtagtg ctcgcacagc gccgcggccg cccggaccgt
    14401 cgagtcgagc gtgctggccg aaccgtccac tccggtgctg accccgacgt cgttgctcgc
    14461 gtcgacgatg accatgacct ggctgtcgtg atcggcgtac gtcgcggtga cgttgaggct
    14521 gttggagcgc agtgacgacg gccagtggat gcgccgcagt cggtcgccga gctggaactg
    14581 ccggatggtc gcgaactcgc tgccgtcgcc gggacgcgcc gaccgatcga tgccgacgag
    14641 cccctccggg tggggcatcg gcgccaccgt gtcgaacacc ggcaccgaag gaagcacctc
    14701 gacatcgcgg ccgtccagga tctgcggacc ccattggtag gcaccccagg cactgagccc
    14761 ggccacggtt gtcggcccga ttgcgtgatg tccccacctc gtcatccgga gcagcagttc
    14821 gacgttcacc gacgactccc cgcgagggac gagacccgca gcgatgccgt atgccggtcg
    14881 ggtccggacc caggggctga ccgtgcagcc gagccagaac tcctgggcgc cgcccggcgc
    14941 ttcgacggtg acgcgcagcg cggtggcctg gccttcacgg atcgtcgtac gccccagtcg
    15001 accggtcacc tgggcgggcc ggcccggccg cgttccggca ccccacacgg cgaccaccac
    15061 gagcggcgta ctcgccacca agaggtccgg acgatggaac accaccgcga cgagcacgcc
    15121 gacgccgccg agcagc
//
LOCUS       DATBFZ010000284         5180 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_2818197, whole genome shotgun sequence.
ACCESSION   DATBFZ010000284 DATBFZ010000000
VERSION     DATBFZ010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 5180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5180
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2818197"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1244
                     /locus_tag="VH419_08770"
     CDS             <1..1244
                     /locus_tag="VH419_08770"
                     /inference="COORDINATES: protein motif:HMM:NF017774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEY7043746.1"
                     /translation="QGLSSMPLTQIPAPLRKSASFAPSKRTKLVGKQIADAVEGDAVF
                     REHLATQVRAGAADLARLLESDEAVSDADLPEVAALAYILRPDGWEDCVDRATAQTLA
                     EVPASAGERTPSEQKLQADLKQARGEVKAVRDKLKGQLEQVKADNTQLRRTLGHTRVQ
                     LKEAQQAAEEARAESDAVRRDAEAAAREADAEQRRLRQRVEQLESENTASRRAQRDDR
                     DAENMRLRLLLDTIMESASGLRRELALPPSEVLPADTVPAVEPGVISHAAGIGRSLLN
                     DDPVLLRRLLEVPRSHLIVDGYNVSKTAWPTAPLDQQRSRLTSSLAALVGGKGIETTI
                     VFDGADLAHPPGVTSPRGIRVRFSPAGVIADDLIRQLVSAEPMGRSVIVVSTDREVAK
                     SVAKMGARSVASAALIKAMGL"
     gene            1241..1789
                     /locus_tag="VH419_08775"
     CDS             1241..1789
                     /locus_tag="VH419_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012919268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HEY7043747.1"
                     /translation="MTVVPVVVGVQASSAHSFSKAQRPVIRLVVGVGVEGDAHAGRTV
                     QHRSRVRRDPTQPNLRQVHLVHAELQDELEQQGFRVTPGDIGENITTRGLDILGLPRG
                     SRLRLGSEAVVEITGLRNPCRQLDDFQPGLMQASLARNAAGGLVRKAGVMGIVVAGGD
                     VRAGDVIGVELPVPPHQALQPV"
     gene            1901..2173
                     /locus_tag="VH419_08780"
     CDS             1901..2173
                     /locus_tag="VH419_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017933923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043748.1"
                     /translation="MGAEAEHEGSVEHFRGQPSGHDGLVIDCDRCSVRGAGCSDCVVT
                     VLLGGPPFGVELDEVEQRAIDVLSDAGLVPPLRMVEAVEGPHIEPA"
     gene            2428..3543
                     /locus_tag="VH419_08785"
     CDS             2428..3543
                     /locus_tag="VH419_08785"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HEY7043749.1"
                     /translation="MSNGHVHRLPPSTKRLALVSAAVLVGSLVLSAPPGVADPSDPGA
                     PAAQKKPTISEVKDRLDSLNRKAEIASEDLNTVRVQMQAAKKRLDALQADVDRKRAHV
                     AALRRLVVGTAVSDYQNGGGLSTTASFLVADDPSAFMDGVASKAVVEHQQAELLSRLT
                     QQQRQLGVQEKQAQREVVAVDKDRELAAQHKAELDKRAQEAQDLLDSLEAAQLRRLER
                     LQRLQEQQTTTTTSTTTTTSPQPSRSTPRVSLSDVPASGRAETAVQTALAQIGDPYVY
                     GAAGPDAFDCSGLTMYSWAAAGVSLSHSSSVQSTQGTPVSLSSLMPGDLVFYYSPVSH
                     VGMYIGNGQVVHAPHPGLSVQIVSLYSMPVNHAMRVG"
     gene            3615..4931
                     /locus_tag="VH419_08790"
     CDS             3615..4931
                     /locus_tag="VH419_08790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043750.1"
                     /translation="MDEHERPAGAWALRQVAVLVAALLVLAAIVYVVQRQRDPGEPTG
                     DRVAGLQDSPSSSELDDFARDVDATHVLAQQAQALAHSSAHSYRQTWDSGRQSQRAAS
                     TIFANLRALQVRRMGLQYVAADPSAASPAGDGAAGAQTWDAQVAVSWRLSGIDRSDAH
                     STLTMTFVRRGDLVKVVAMSARPGEREPIWLTGPLRVLRSDRTVVAATNMVDARRIDQ
                     ELRRAAIDVRKVIPSWRGSLAAYAPATREQFDALMGANPDAYAGIAAVTTTVDGSRRA
                     QAPVAIVVNPVVFQRLGPIGRHVVVTHESTHVATGATTVTMPLWVAEGFADYVGVGSV
                     DVPLRVAAQAAIRDARLHGPPTSLPSDADFSSTDRPVEVAYEQAWLALRAIADEQGRD
                     RLVAFYRDVVDHPRAVWRLLRRDAGMTRVELTRDWRANLEALVDGR"
     gene            4921..>5180
                     /locus_tag="VH419_08795"
     CDS             4921..>5180
                     /locus_tag="VH419_08795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043751.1"
                     /translation="MAGRSPTRMAVIAFVVLLATLVVMIVLLIPWRLPLEGAPAKASS
                     YFTAAQIQRSEAFHDAAKWPAWLGLVASLAGAVWMGFGGLGRR"
BASE COUNT          822 a         1755 c         1810 g          793 t
ORIGIN      
        1 cacagggtct gagcagtatg ccgctcacgc agatcccagc gcccctgcgc aagtcggcct
       61 cgttcgcgcc gtcgaaacgc accaagctcg tggggaagca gatcgccgac gccgtcgagg
      121 gtgacgcggt gttccgcgaa cacctcgcca ctcaagtgcg cgcgggcgcg gcggatctgg
      181 cgcggctcct cgagagcgac gaggcggtga gcgacgcgga tctgcctgag gttgccgcac
      241 tggcctacat cctccggccc gatgggtggg aggactgcgt cgaccgtgcc acggcccaga
      301 ccctcgccga agtgccggca tcggccggtg agcggacacc cagtgaacag aagctccagg
      361 ctgacctcaa gcaagcccgc ggcgaggtca aggccgtgcg cgacaagctc aagggccaac
      421 tggagcaggt caaggccgac aacacccagc tccgacgaac gcttggacac acccgggtgc
      481 agttgaaaga agcgcagcag gctgcggagg aggcacgcgc ggagagcgac gccgtacgcc
      541 gggacgccga ggcggcagcg cgcgaagccg acgcggagca gcgccggctt cggcagcgcg
      601 tggagcagct cgagtcggag aacaccgcca gccggcgcgc gcagcgtgac gaccgcgacg
      661 cggagaacat gcgcctccgg ctgctcctgg acacgatcat ggagagcgcc tccggcctgc
      721 gtcgcgagct cgcgcttccg ccgtcggagg tgctgcccgc agacaccgtg ccggcggtgg
      781 agcccggcgt catctcgcat gccgcaggca tcggccgatc actgctgaac gacgacccgg
      841 tcctgctccg tcgcctgttg gaggtgccgc gctcacacct gatcgtcgac ggctacaacg
      901 tgtccaagac ggcatggccg acggcgccgc tcgaccagca acgttctcgt ctcaccagca
      961 gtcttgccgc tctcgtcggc ggcaagggga tcgagaccac gatcgtgttc gacggcgccg
     1021 acctggcgca tcctcccggg gtcacgtcgc cgcgtggcat ccgggtccgg ttcagcccgg
     1081 ccggcgtcat cgcggacgac ctcatcagac agctcgtgtc ggccgaaccc atgggtcggt
     1141 cggtgatcgt ggtgtccacg gatcgggagg tcgccaagag cgttgccaag atgggcgcgc
     1201 gctcggtggc gtcggctgcg ttgatcaagg cgatgggctt gtgaccgtgg tgccggtcgt
     1261 cgtcggcgta caggcatcgt cggctcacag cttcagcaag gcgcaacgtc ctgtgatccg
     1321 gctcgtggtc ggcgtcggcg tcgaggggga cgcccacgcc ggcaggacgg tccagcaccg
     1381 ttctcgcgta cgccgcgatc ccacgcaacc caacctgcgc caggtccatc tcgtgcatgc
     1441 cgagctccag gacgagctcg agcaacaggg atttcgggtg acgcctggag acatcggcga
     1501 gaacatcacg acccggggcc tcgacattct cggacttcca cgcggcagca ggctgcggct
     1561 gggcagcgag gcggtcgtcg agatcaccgg gttgcgcaac ccctgcagac agctcgacga
     1621 cttccaacct ggcctgatgc aggcctccct cgcccgcaac gccgcgggtg gcctggtgcg
     1681 gaaggccggc gtgatgggga tcgtcgtcgc cgggggagat gtgcgcgcgg gcgacgtcat
     1741 cggcgtcgag ctcccggtcc caccgcatca ggcgctccaa cccgtctgat cgtcggcgtg
     1801 tcgccgccac cgggaactgt cggtgggccg ccgtagcgtc actcgtgaca tccgacgagg
     1861 gcgggcagca gggctggatc gccgatgcgt ggaggacacg atgggcgctg aagcagagca
     1921 cgagggctcg gttgagcact tcagaggcca gccctccgga cacgacgggc tggtgatcga
     1981 ctgcgaccgg tgcagcgtgc gaggggctgg atgtagtgac tgtgtcgtga ccgtgctgct
     2041 cggcggtccg ccgttcgggg tcgagctcga cgaggtcgaa cagcgggcga tcgacgtctt
     2101 gtccgacgcc ggcctggtgc ccccgctgcg gatggtcgag gccgtggagg ggccgcacat
     2161 cgagccggct tgagggccaa cgctgctgca aagatgtggg tttgccagag tcggatgggt
     2221 cagaagtgat cagagtggct ccgtgagcaa ggtcactcct ccgaaatctg agaatcgcgg
     2281 ggaggggttg gaggccttcc agcgctctcg ctagggtgtg ttcccggttg ccgtcatgcc
     2341 ggtctcgtgt ttggctgaag gctactcagc aggagcgggg gcgtctgctg ttgccgtgtc
     2401 cgctacccag agggagaccg tcgacccgtg tcgaacggcc acgtccaccg cttgcctccg
     2461 tccaccaagc gcctcgcact cgtgagcgct gctgtgctcg tcggaagcct ggtgctctcc
     2521 gcgccacccg gcgtcgctga cccgagtgac cccggtgccc ccgcagctca gaagaagccg
     2581 acgatctccg aggtcaagga ccggctcgac agtctcaacc gaaaggcaga gattgcctcg
     2641 gaggacctca acaccgtccg agtccagatg caggctgcca agaagcggct cgacgccctg
     2701 caggccgatg tcgaccggaa gcgggctcac gtcgcggcgc tgcgccggct cgtcgtcggc
     2761 acggccgtgt cggactatca gaacggcggg ggactctcca cgaccgcgtc cttcctggtg
     2821 gcagacgacc ccagcgcttt catggacggc gtcgcctcca aggcggtggt cgagcaccag
     2881 caggcggagc tgctgtccag actgacgcag caacaacggc agctcggcgt ccaggagaag
     2941 caggcgcaac gcgaggtcgt ggcggtggac aaggaccgtg agctcgccgc gcagcacaag
     3001 gccgagctgg acaagcgggc gcaggaggcg caagacctgc tcgacagtct cgaagccgcg
     3061 cagctgcgcc gcctcgagag gctgcagcgg ctgcaggagc agcagaccac gaccaccacg
     3121 agcacgacga cgacgaccag ccctcagccg tcgcgcagca cgccgcgggt ctcgctcagc
     3181 gacgttcccg cctccggccg cgccgagacc gcggtccaga cggcgctggc tcagatcggc
     3241 gatccctacg tctacggggc tgctgggccc gatgcgttcg actgctccgg gctgaccatg
     3301 tactcctggg cagcggccgg cgtctcgctc tcacactcct cgagtgtcca atccacgcag
     3361 ggcacccccg tgtcgctgtc gtccctgatg ccgggcgacc tcgtcttcta ctactcgccg
     3421 gtgtcgcacg tcggcatgta catcggcaac ggtcaggtcg tgcatgcgcc gcacccggga
     3481 ctgtcggtcc agatcgtgtc gttgtactcg atgcccgtga accacgccat gcgcgtgggc
     3541 tgacctccca cccgcctgga gagctttccg cgacgcgatc aatcccaggt gcacgacgca
     3601 cgtaggatga cgcggtggat gagcacgagc gcccagccgg agcctgggcg ctccgtcagg
     3661 tcgccgtcct cgtggccgca ctcctcgttc ttgcggcgat cgtgtacgtc gttcaacgtc
     3721 aacgtgaccc gggggagccg acgggggatc gggtggccgg cctccaagac tcaccgtcct
     3781 cgtccgagct cgacgacttc gctcgcgatg tcgacgcgac ccacgtcctc gcccaacagg
     3841 cgcaggcgct cgcacacagt tccgcacact cctatcggca aacgtgggac tcaggccgcc
     3901 agtctcagcg ggcggcctcg acgatcttcg ccaacctgcg cgctcttcag gtccgacgga
     3961 tgggcctgca gtacgtcgcc gccgatccaa gtgccgcctc gcctgccggt gacggggcgg
     4021 ccggcgctca gacctgggac gcccaggtcg ccgtctcatg gcgactctcc ggcatcgacc
     4081 gtagcgacgc ccactcgacg ctgaccatga cgttcgtgcg tcgtggtgac ctcgtcaagg
     4141 tcgtcgcgat gagcgctcgc ccgggcgagc gcgagcccat ctggttgacc ggcccgctgc
     4201 gggtccttcg cagcgaccgc accgtcgtcg cggcgaccaa catggtggat gctcggcgca
     4261 tcgaccagga gttgcggcgg gccgcgatcg acgtcaggaa ggtcatcccg agctggcgcg
     4321 gcagcctggc ggcgtacgcc cccgcgaccc gcgagcagtt cgacgcactg atgggagcca
     4381 atccggacgc ctacgccggg atcgccgccg ttacgacaac cgtcgacggg tcgaggcgcg
     4441 cgcaggcgcc cgtggcgatc gtcgtcaacc cggtggtgtt ccagcggctc ggcccgatcg
     4501 ggcggcacgt cgtggtcacg cacgagtcga cgcacgtcgc caccggtgcg acaacggtga
     4561 ccatgccgtt gtgggttgcg gagggatttg ccgactacgt aggggtcggt tcagtcgacg
     4621 taccgctccg cgtcgctgcg caggccgcca tccgcgacgc gaggttgcac ggcccgccca
     4681 cgtcgttgcc gagcgatgcc gacttcagca gtaccgaccg gccggtcgag gtggcatacg
     4741 aacaggcgtg gttggccctg agggcgatcg cggacgaaca gggccgggat cggctggtgg
     4801 cgttctaccg ggacgtggtc gatcatccac gagcagtctg gcgcttgttg cgtcgggacg
     4861 ccgggatgac cagagtcgag ctgaccaggg actggcgggc gaacctggaa gcactcgtcg
     4921 atggccggta ggtcgccgac ccggatggcg gtgatcgcgt tcgtcgtcct tctcgcgacg
     4981 ctggtcgtca tgatcgtgct gctgatcccg tggcggctgc cgctcgaggg cgcccccgcc
     5041 aaggccagct cctacttcac cgcagcgcag atccagcgtt cggaggcatt ccacgatgcg
     5101 gcgaagtggc cggcctggct ggggctggtc gcgagcttgg cgggcgcagt gtggatgggc
     5161 ttcggcggtc tcggtcgccg
//
LOCUS       DATBFZ010000285         3161 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_1183316, whole genome shotgun sequence.
ACCESSION   DATBFZ010000285 DATBFZ010000000
VERSION     DATBFZ010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 3161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3161
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1183316"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /locus_tag="VH419_08800"
     CDS             <1..618
                     /locus_tag="VH419_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HEY7043752.1"
                     /translation="TYGQIRVIDVNESQQQGPSQVANNMRSDPEVSAELAQFNRSGNS
                     ISYGNLLTVPVGDELLYVEPVYVRLATANEANFPILRNVLVSYKGGVGIGQSLETALF
                     AALQDTEPGSPSATPTPSGTPSPSGPTSSPTSSAEPGSVHQLLDQAQAQFVLADQALK
                     EGNLSEYQDHIQRAQDLVNRAIALENGASSSSPSAPSSSGTSPSP"
     gene            662..738
                     /locus_tag="VH419_08805"
     tRNA            662..738
                     /locus_tag="VH419_08805"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:696..698,aa:Met,seq:cat)
     gene            1862..2077
                     /locus_tag="VH419_08810"
     CDS             1862..2077
                     /locus_tag="VH419_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043753.1"
                     /translation="MQERANAAGFDGMPVSGHSLRAGHGTSAAVNGAPIDRIAAQTRH
                     RDLGTLLNHDIRPAEATATTTSRDLGL"
     gene            complement(2375..>3161)
                     /locus_tag="VH419_08815"
     CDS             complement(2375..>3161)
                     /locus_tag="VH419_08815"
                     /inference="COORDINATES: protein motif:HMM:NF025008.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA thioesterase domain-containing protein"
                     /protein_id="HEY7043754.1"
                     /translation="WHPTPAATSQWASGSTMRGQASSALMARAAEQTIGPASGLRPAR
                     WTADLSRPVPMRPSTVQATVLRSGRRLRSVDVELIQEGSVMARARALFLAVDNDVAGQ
                     NWETTHSLALPPPDMTPDTPEPRLYYSEAENWSTRAAPHRNNSRKQMWTFPFSVVEHE
                     RATPFQFAAGAADLASPVSFWEAAGISYINADITLALARLPGAGGIGVAAAGRVASSG
                     IAVGTVLMHDREGIFGTATVSAIWSGDRRVDPGDRDERSRGVG"
BASE COUNT          538 a         1118 c         1013 g          492 t
ORIGIN      
        1 acctatggcc agatccgggt catcgacgtc aacgagtcgc agcagcaagg accgagccag
       61 gtcgccaaca acatgcgctc cgacccggag gtctccgccg agctcgcgca gttcaacagg
      121 tcgggcaact cgatcagcta cggcaacctg ctcacggtgc ctgtcggcga tgagctcctg
      181 tacgtcgagc cggtctacgt ccgactggca accgccaacg aggcgaactt cccgatcctg
      241 cgcaacgtgc tggtctccta caagggcggc gtcggcatcg ggcagtcgct cgagaccgcg
      301 ctcttcgcgg cgctccagga caccgagccg gggagcccgt ccgcgactcc cacgccgtcg
      361 ggcacgccgt cgccatccgg tccgacgagc tcacccacgt cgtccgccga gcccggctcg
      421 gtgcaccagc tcctggacca ggcccaggcg cagttcgtcc tcgccgacca ggcgctcaag
      481 gaggggaacc tgtcggagta ccaggatcac atccagcgcg cccaggacct cgtcaacagg
      541 gccatcgcgc tggagaacgg cgcctcgtcg tcgtcaccct cggccccgtc gtcgtcgggc
      601 acctcaccct caccctgatt tggggtcacc gccactggtg ccgtaacctt gggttcaccg
      661 acgcggggtg gagcagctcg gtagctcgct gggctcataa cccagaggtc gaaggttcaa
      721 atcctgcccc cgctaccaaa ggaaagtgca ggtcagagct gggttcgcga ctcaccgcgg
      781 gcccggcctc ttgcttcgtg tcagcaggat gtctgctgac attgcgcgga catggcgcgg
      841 acatctgccg ccgctggcgg ctatctcccc ggctgcatgg cgataaacgg cgacctcttg
      901 accaatagcc cagaggtcgc aggttcaaat cctgcccccc gctacaaagt ccaggcgtgg
      961 gatccagtcg gatcccgcgc ctgaaagctt ttggtttgag ctaccttcca caaccctcct
     1021 tttgggaggt gagtcgcgac tccggccgcc gttcgcccgg gcctggggga tccgtcgccg
     1081 agattggcgc gtgacgactc caccgatcac cccggaagaa ccgccactga cgcgcgccgt
     1141 cgtggcgctg accgcgctcg atgccgaacg catcggcgcc gccgttgacg ccgagctcgc
     1201 cgcctcgacc cgcgagactt acgcctgcgg ctggcgacaa tgggagcgct ggtgtcgcgg
     1261 caggggcctc aacccgctgc cggcacgacc cgaggcggtc gcggcgttcc ttacccgagc
     1321 gcgccgaggc aggtgtccac ttcaccaccc tcgactgcga ttgctccggc attgcctatc
     1381 gccaccgcca ggagggactg gcggacccga ccgccgactt cctcgtccga cgggtccgtc
     1441 gagggctgcg ccggatcctg ggggtcgcgc cgatccggca ggctcacccg ctcaccgttg
     1501 tcgagttcgg ccggatcgtc gcgtccatcg aaaccgatga cgccagggac ctccgtgacc
     1561 gggccatcct gctcctcggc tacgccttcg cgatgaggcc tggcgagatc tcgacgtcga
     1621 ggacctccag cgccagccga ccggcgtcct gatcagaatc cgccgatcca agaccgaccc
     1681 cgacgcccac ggccaactgg tcggcgtcgc ccgtggggac aaccgggtca ccgacccgat
     1741 ccgcgccctc gatgcctggc tcaaggtccg cccctcagaa ccgggtgcgc tcttcacgcg
     1801 cgtcctgtga aggaaccatc ccaccagcga gcggatcggc ccgcgcgcga tcagcaggac
     1861 cgtgcaggaa cgagccaacg cagccggctt cgacggtatg cccgtctcag gccactcgct
     1921 gcgcgccggg catggcacct cggcggccgt caacggcgca ccgatcgaca ggatcgccgc
     1981 gcagaccagg caccgcgacc tcggcaccct gctcaaccac gacatccgac ccgccgaagc
     2041 gacggcgacc accaccagcc gcgacctcgg gctttgaggg actgccgcgc ctggaggccg
     2101 cactgacgca cgggtggtcc catatctggc agaacaggtg gccccgtgaa gctggcagaa
     2161 aaccgctcag ggcggtccca cgctcatggc aggcgacagc tggcatctcc gaactccatg
     2221 gaagcgaagg attcgccccc gtggaacaac cactcgtctc gaggaaccac tggggcggta
     2281 cctcgacgac gacctcgacc gaatggaaca acaagcgctc gatgagttcc gagggaagta
     2341 gaccgactcc ttcgccgcgc cgcagcgcgg tgcatcagcc gacgcctctc gaacgctcgt
     2401 cacggtctcc gggatcgact cgtcggtcgc cgctccagat tgcgctgacg gtggcggtcc
     2461 cgaatattcc ttcccggtca tgcatgagga ccgtacctac ggctatgcct gacgaggcga
     2521 cgcgaccggc ggcagcgacg ccgatgcctc cggcaccggg caggcgtgcc agtgccaacg
     2581 tgatgtcggc gttgatgtac gagataccag ccgcctccca gaacgagacc gggctggcaa
     2641 ggtccgccgc cccagccgcg aactggaacg gggtcgcccg ctcgtgctcg accacggaga
     2701 acgggaaagt ccacatctgc ttgcgtgagt tgttgcgatg aggtgccgca cgagtcgacc
     2761 agttctcggc ctcgctgtag tagaggcgag gctccggggt gtctggcgtc atgtcgggag
     2821 gtggtagagc caggctgtgc gtcgtctccc agttctgacc ggcgacgtcg ttgtcgacgg
     2881 ctaggaagag cgcacgagct cgtgccatga cggagccttc ctggatcagt tcgacgtcga
     2941 cagagcgcag tcgtcgacct gagcgcagga cggtcgcctg gactgtgctc ggccgcatcg
     3001 gtactggccg cgataagtcg gcagtccagc gtgcgggccg aagaccggac gcgggaccga
     3061 tggtttgttc ggcggcacgg gccatcaggg cgctggacgc ttgtccacgc atagtgctgc
     3121 cggacgccca ttgactggtt gcagcggggg ttgggtgcca t
//
LOCUS       DATBFZ010000286         2324 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_3477986, whole genome shotgun sequence.
ACCESSION   DATBFZ010000286 DATBFZ010000000
VERSION     DATBFZ010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3477986"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(132..1196)
                     /locus_tag="VH419_08820"
     CDS             complement(132..1196)
                     /locus_tag="VH419_08820"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY7043755.1"
                     /translation="MGVDQVIAARDRVLAADAVERPLPELLSDVLDALHGVATFSVAA
                     LLLTDTDSMLPFGGVVEGLDPSQCTPFWDNELLDPDFAKFTSLARSAEPVATLSQLTD
                     GDLGRSPRYRKLYEPAGGGDEIRVAFTTGTSCWAVAALVRSADLGPFPAEESQAVLEV
                     VPVVARAIRSAVMRRDRATAAPAPTMLVLDSDASLVSATPGAADVLAELATNGVDIDV
                     PTVVLAATRRAKNLKNATGISMRARGSSGRWFRLHAAPLGDDEVAVVVAPAGPADLVS
                     IVIESYGLTERESEVVPLLARGMSTKEIASEMCISRHTVNDHIKMIFSKCGVSSRGEL
                     VARLFADHILEHHHAATVHL"
     gene            1330..2163
                     /locus_tag="VH419_08825"
     CDS             1330..2163
                     /locus_tag="VH419_08825"
                     /inference="COORDINATES: protein motif:HMM:NF039346.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY7043756.1"
                     /translation="MAYEPAAPTIIRRPARIADETWLIHQVQEALGAPLCVYLNSMVI
                     RGAEPLIVDTGTVANRAQWLDDVFGLVDPADVRHVFVSHDDVDHTGNLAQVREHCPNA
                     TLLASWALVERFSNAFEFPLQRCRWVDDGSHLQLDDRRLHFVRPPLWDSPTTRGVFDT
                     STDVYWAVDAFATPCSTAVEASVDELDPETWRGGTAMFAHHALSPWLAFVDNGRYRDH
                     VEHVRALGATTIASAHSPLITGAAVDTAFDLLVDLPDVEVPPAPDQAVLDAIVSQLPA
                     A"
BASE COUNT          361 a          785 c          796 g          382 t
ORIGIN      
        1 ggctggagct ttcgcgctgg tcggctggga gccgcgttcc tgcggtatcg ccattggcga
       61 tcccatgaaa tcctgtgacg aggctcaaga gcactcaagt cgtaagagag ggaagcgccg
      121 tccgtgggtg ctcagaggtg aacggtggct gcgtggtgat gttcgaggat gtggtcggcg
      181 aacagtcttg cgacgagttc gccgcggctg ctcacgccgc acttggagaa gatcatcttg
      241 atgtggtcgt tgacggtgtg gcgcgagatg cacatctcgc tggcgatctc tttggtcgac
      301 attccccggg cgaggagcgg caccacttcg gactcgcgtt cggtgaggcc ataggactcg
      361 atgacgatgg agacgaggtc ggctggacca gcgggcgcga cgacgacggc gacctcgtcg
      421 tcgccgagcg gagccgcgtg gaggcggaac cagcggccgg agctgccgcg tgcgcgcatc
      481 gagatcccgg tggcgttctt caggttcttg gctcgtcgcg ttgctgccag gacgaccgtc
      541 ggaacgtcga tgtcgacgcc gttcgtggcg agttcggcca gcacatcggc cgcgcccggc
      601 gttgccgaga cgagtgacgc gtcgctatcg agcacgagca tggtcggcgc cggcgcggca
      661 gtggcgcggt cgcggcgcat cacagcggag cgaatcgctc gggcgacgac cggaacgact
      721 tcgaggaccg cttgcgattc ttcagccggg aacggtccca ggtcggcgct gcggaccagt
      781 gcggcgaccg cccagcacga tgtgccggtg gtgaacgcca cccggatctc gtcgccgccg
      841 ccggcgggtt cgtacagttt gcggtagcgc gggctgcgcc ccaggtcgcc gtcggtcagt
      901 tgtgacagcg tggcgaccgg ttcggcacta cgcgccagag acgtgaactt ggcgaagtcg
      961 gggtcgagca gctcgttgtc ccagaacggc gtgcactggg aggggtcgag cccctcgacg
     1021 acaccaccga agggcagcat cgagtcggtg tcggtcagca gcagcgctgc gaccgagaat
     1081 gtggcgacgc cgtgcagcgc gtcgagcacg tcgctgagca gctcgggcag cggccgctcg
     1141 acggcatcgg cggcgagcac gcggtcccgt gcggcgatga cctggtcgac gcccactcgg
     1201 tggagggtag gacgggtcaa gggtccggtc atccccctcg ttcgtggggg tccacccccg
     1261 ccgagcaacc cggtgaaacc cgcgatcacg ggattcacgg tgcatcgacc catccgtacc
     1321 ctcgccctca tggcttacga acctgccgct cccacgatca tccggcgtcc tgcgcggatc
     1381 gccgacgaga cgtggctgat ccatcaagtg caagaggcac tcggtgcgcc gctgtgcgtg
     1441 tacctgaact cgatggtgat ccgcggcgcc gagccgctca tcgtcgatac cggcaccgtc
     1501 gccaaccgtg cccagtggct ggacgacgtg ttcgggctgg tcgatccggc cgatgtgcgc
     1561 cacgtctttg tctcccacga cgacgtcgat cacaccggca acctcgccca ggtcagggag
     1621 cactgcccga acgcgacgct gctcgcatcg tgggcgttgg tcgagcggtt ctccaacgcc
     1681 ttcgagttcc cactgcagcg ctgccgttgg gtcgatgacg gatcgcacct gcagctcgat
     1741 gatcgccgcc tgcacttcgt gcggccaccg ctgtgggact cccccacgac acggggcgtc
     1801 ttcgacactt cgacggacgt ctactgggcc gtcgacgcct tcgccacacc atgctcaact
     1861 gcggtcgaag cgtcggtcga cgaactcgac ccggagacct ggcgaggcgg gacagcgatg
     1921 ttcgcccacc acgcgctgtc cccgtggctc gccttcgtcg acaacggccg ctaccgcgac
     1981 cacgtcgaac acgtgcgcgc gttgggcgcc accaccatcg cctcggcgca cagtccgctg
     2041 atcaccggcg ccgcggtcga cacggcgttc gatctgctcg tcgacctgcc ggacgtcgag
     2101 gtgccgccgg cacccgacca ggcagtgctc gacgccatcg tctcccagct ccccgccgcc
     2161 tgagccgtac gtgcgcatcg gcagccgact gccacaactc ggtcgctgcg ccgacatggc
     2221 gttgacgggc ggcgcgctcg tgccgactcg ccgggacgcg cgcctctcgg cgctcgacaa
     2281 cgcaccggcc gacacgaagt tcatgctggc gatgcacaac gcct
//
LOCUS       DATBFZ010000287        11552 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_1836714, whole genome shotgun sequence.
ACCESSION   DATBFZ010000287 DATBFZ010000000
VERSION     DATBFZ010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 11552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11552
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1836714"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(144..476)
                     /locus_tag="VH419_08830"
     CDS             complement(144..476)
                     /locus_tag="VH419_08830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043757.1"
                     /translation="MSSALMVLAYDANDAKPGWIALIVVVLLGLATFLLWRSMNTQLR
                     KISAPYQADLDAQRDQRDSNHEPRRDSDREARRDGTHELDPDHNPDPNHNPERNADPT
                     DPTRDVEP"
     gene            complement(473..1387)
                     /gene="mca"
                     /locus_tag="VH419_08835"
     CDS             complement(473..1387)
                     /gene="mca"
                     /locus_tag="VH419_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013161572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010126 - mycothiol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycothiol conjugate amidase Mca"
                     /protein_id="HEY7043758.1"
                     /translation="MELTNGLRLMHVHAHPDDESSKGAASTAKYVAEGVDVHVVTCTG
                     GERGSILNPNMDRPDVLANITEIRRQEMDRAREILGVTQDWLGFVDSGWPEGDPKPPL
                     PDGCFALVPVEEAAEPLVRLMRSFRPHVVTTYDENGGYPHPDHIMCHQVTLHAWKAAA
                     DPTAYPEAGEPWQPLKLYYHHSFNRPRIEALHEAMVARGLESPYEEWLEEWKDDPEHD
                     DRVTTRVPCAEYFHIRDMALIAHATQIDPEGRWFAIPLDVSQRAWPTEDYELVESLVP
                     SELPESDLFAGIRALVAAYDGRRESIGA"
     gene            1552..1983
                     /locus_tag="VH419_08840"
     CDS             1552..1983
                     /locus_tag="VH419_08840"
                     /inference="COORDINATES: protein motif:HMM:NF025520.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4307 domain-containing protein"
                     /protein_id="HEY7043759.1"
                     /translation="MTSPGDARPPGAAPSRQPLQERYGVRSPRDRRVLQVSVAAVALA
                     FLAWLGWAAWDHSHPDVTGQLHGYDVVSPHQVRVVIDITRTDGQHVVCDVLAQAEDHS
                     AVGETQVAVRAGGADTVQVSAVIRTDREATTATVSNCRAAD"
     gene            2154..2660
                     /gene="greA"
                     /locus_tag="VH419_08845"
     CDS             2154..2660
                     /gene="greA"
                     /locus_tag="VH419_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEY7043760.1"
                     /translation="MTQPATEKAIWLTQEAYDGLAAELEHLKGPARAEISQRIADARE
                     EGDLRENGGYHAAREEQSKTEARILQLEDMLSRAKVGQTPDEGDGTIAAGMEVTVRFT
                     DGDTERFLLGAREVLAHDTVDVSVYSPQSPLGAAIVGKSAGDSATYAAPNGNKVTVEI
                     VEAKPFTG"
     gene            complement(2727..3977)
                     /gene="ilvA"
                     /locus_tag="VH419_08850"
     CDS             complement(2727..3977)
                     /gene="ilvA"
                     /locus_tag="VH419_08850"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006549 - isoleucine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HEY7043761.1"
                     /translation="MDTEESGPAASTAEVPKVTLEQIQAAREELRGVALVTPMEGSRW
                     LRALTGQDVWLKCENLQRTGSFKIRGAYLRISHLSEEERARGVVAASAGNHAQGVALA
                     ASLLGIKSTVFMPIGAPIPKEKATRSYGADVEFVGHAIDSCLIEAHRFQAQTGSALIH
                     PFDHVDIVAGQGTCGLEIVEQVPEVKTIVVPTGGGGFLAGIATAAKSLRPDVRVVGVQ
                     AESAAAYPASLAAGHPVPLSSMATMADGIAVGCPGEVPFAAIQTYVDEIRTVSEESLS
                     TALLALMERAKVVVEPAGAAAVAALLDRPEDFEGPVVAVLSGGNIDPLLLMRVIRHGM
                     AAQGRYLALECRISDRPGGLVKLLSELSACDANVLDVVHERNSESLPLDQVDVVLQME
                     MRGQEHRDLVLAKLRERGYDVKVF"
     gene            4064..4540
                     /locus_tag="VH419_08855"
     CDS             4064..4540
                     /locus_tag="VH419_08855"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY7043762.1"
                     /translation="MQAKPFDLEQPQSPEEAVIGLLMQAGRRLRTRHPEDQVDPSTFP
                     LAKQLMCHDGMRLSDLAAKVELDASTVSRQIKQLEDKGIVERTPDPADGRASLVRLTD
                     EGHRTIQAGFRRRFERIRSALEPWSQQDRDVLQKLLTRLAADLRNANDLHESAHSA"
     gene            4537..6096
                     /locus_tag="VH419_08860"
     CDS             4537..6096
                     /locus_tag="VH419_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HEY7043763.1"
                     /translation="MTQTPQTHTADDHGYLSHRQILIVLGGLMAGMFLAALDQSIVGT
                     ALPRITSELGGLAKLSWVVTAYLLTQTASTPLWGKISDLYGRKLIFQTAIITFIFGSL
                     MSGLAQNIGELIAFRAVQGLGGGGLFALALATIGDIVPPRERGRYGGYFGAVFGTSSV
                     LGPVLGGFFADGPGWRWIFFINVPIGLVALVITSAALRIPVVRREHAIDYVGAATIVA
                     AVSSLLLYTTWAGPDHGWASGLGLTLLGAGFVLTGLFVYVESRAAEPILPLDLFRISV
                     FRTANLYSFIIGFAMFGGIIFLPVYLQVVQGMSPTESGLALLPMVVGIFSTSISSGIV
                     MSRTGRYRLFPIIGAVTVIAALVMLTRLDVGSHYWFAAIGFYIFGAGLGFSMQIIVTI
                     VQNAVDRRHMGSATSAVAFFRSMGGTFGAAVFGAILSSRLAAHLADEFAGQGGGAALP
                     AGGDVTNNVERIQHLPEPIHGLVLRAFADSLHDVFLWAIPVVVVALLVAFFIKELPLK
                     MRETSELSEPH"
     gene            complement(6106..7473)
                     /locus_tag="VH419_08865"
     CDS             complement(6106..7473)
                     /locus_tag="VH419_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012923201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HEY7043764.1"
                     /translation="MSLVFCVDFGSTFTKAALVDVDAGRLVATDDHPTTIHTDVLDGW
                     DVIRARLAPLTDGADVPVLACSSAGGGLRIGVVGNEELVTAEAGRRVALSSGGRVVHV
                     SYDEVDLRALRRSRPDVVLLVGGTDGGNADVLRRNARSLAVANWAAPVVLAGNTDVSD
                     DVAALFDAAGVPYVVSANVVPQIGKLAPEGARGAIRKMFLRHVIGGKHLSKRTDFTAM
                     VKGATPDVVLTAVELLGSGLGPERPGVGDVVVVDVGGATTDVHSVVEVDPEEAGLGRE
                     VVETVPVSRTVEGDLGMRWSAVPTVEAARAAGLDLADDVAAAAKVRRTDPSMIPVTPT
                     ELEDDVAIASAAVQLAVRRHAGRQQVVFSPDGRLVERSGKDLREVGLLVGSGGVLRHN
                     AADVAARILAAGTGDHVAGGWLVPRSPRVVVDLDYVLAAAGLLASEHPTAAYDLLLNR
                     VVRNP"
     gene            complement(7489..8646)
                     /locus_tag="VH419_08870"
     CDS             complement(7489..8646)
                     /locus_tag="VH419_08870"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020385497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEY7043765.1"
                     /translation="MSSAYGFETKAIHAGQRADPNNGAVMTPIYAVSTYKQDGVGGLR
                     DGYEYSRTANPTRTALEECLAALEQGTRGVAFASGMAAEDALLRVVCSPGDHVVIPND
                     AYGGTYRLFKQVLEPWGLDHTTASVHDTAAIAAAVRPGRTRVIWIETPSNPLLGVADI
                     PALAQIAHDSDAILVVDNTFATPYLQRPLTLGADVVVHSTTKYAGGHSDVVGGAVVTA
                     DEALGAELALFQNATGATAGPFDAYLVLRGLKTLPVRMDRHCDNTERIVDFLVRHDAV
                     GDVFYPGLVDHPGHQVAAAQMKRFGGMVSFRVDAGNAAALEVCNRARVFTLAESLGGV
                     ESLIELPATMTHASASGSELEVPDDLVRLSVGLETVEDLVADLEQALVGRG"
     gene            complement(8657..9628)
                     /locus_tag="VH419_08875"
     CDS             complement(8657..9628)
                     /locus_tag="VH419_08875"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GNAT family
                     N-acetyltransferase/NUDIX hydrolase"
                     /protein_id="HEY7043766.1"
                     /translation="MVDDGQNFRVVQLDPEAHRRQLSELWRAATAARREAVGLPPLDE
                     TDADVLSRPGAFGVGLVERSMLLAIAVAMPARADDGRSPRQVPGLLHISSVATRPGSW
                     GRGFGRRVVQAILSQGKRRGFARAQLFVHSDNAGARHLYESLGFTWSRRAAVDSNGED
                     IVHYVIDLVATPAEPRPAARLLCCDPRNRVLLLHWRDPYDGFELWEPPGGGIEEGEDP
                     RTAVLREWAEETGLAAPGLVGEPVVVGRDLFWLGERFVCDEFFFLGRAASADDPDVSG
                     QTEIEHASYLGHAWIGWDEIADLGGLDKPDVVAVLRRLDPEGPWKST"
     gene            9742..11109
                     /locus_tag="VH419_08880"
     CDS             9742..11109
                     /locus_tag="VH419_08880"
                     /inference="COORDINATES: protein motif:HMM:NF005453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaminase"
                     /protein_id="HEY7043767.1"
                     /translation="MSSPAGRAVDPDRVQVLKEREDRAFVAARPRSVLLGKQALGVMP
                     NGVPMSWLRTSYDHPPLFVDQASGSRFRDVDGHEYADFNIADMSMFTGYGPPAVVDAV
                     SRRMAAGSQFLLPTEDSIVVAGELGRRYDLPLWQFTLAATSANTEAIRIARVATGRES
                     VLFFDGKYHGHFDEALVEVVDGRVQPEEAGLPYDVTTRTVVVPFNDPDLLERALASRQ
                     VALVITEPALTNNVGLLLPEGDFHVRLRELTSSTGTLLAYDETHTQVVGPGGLTRRWG
                     LQPDIVTIGKSIASGVPLGAYGMTEEVADVLQRPGGRDDDKPQVAVGGTLFGNALSMA
                     AARATMSEVLTDDAYEHTQVLGARLADGLSDLIASARLPWTVHRFWPRSGLTFASAMP
                     RNAVEATASFDVPLRRLMRVYLANRHVWDAIVGAGPTCSVAATTDDVDQYLAAMAELL
                     SELVA"
BASE COUNT         1799 a         4063 c         3840 g         1850 t
ORIGIN      
        1 ggccacctgc gcgatcggcc taccggtctc gatcaccagc gccaccgcgc cctgcttgaa
       61 gtcctcgtcg aacttccgat agttcctgcc catcgcccga cctcctcaaa ggcgatgtct
      121 ccacgctacg aggggaagcc cactcacggt tcaacgtcgc gggtggggtc ggtggggtcg
      181 gcgttgcgtt cggggttgtg gttggggtcg gggttgtggt cggggtcgag ttcgtgggtg
      241 ccgtcgcgtc gggcttcgcg gtcgctgtcg cgtcggggtt cgtggttgct gtcgcgttgg
      301 tcccgttgag cgtcgaggtc tgcctggtac ggcgcggaga tcttgcgcag ctgggtgttc
      361 atgctccgcc acagcaggaa cgtcgccagc cccagcagca ccacgacgat gagcgcgatc
      421 cagcccggct tggcatcgtt cgcgtcgtac gccagaacca tcaacgcgga gctcatgctc
      481 ctatgctctc gcgccgcccg tcatacgcag caacgagggc acggatgccg gcgaagaggt
      541 cggactccgg cagctcgctg ggcaccaacg actcgaccag ttcgtagtcc tcggtcggcc
      601 aggcccgctg actgacgtcc agcgggatcg cgaaccaccg gccctcggga tcgatctggg
      661 tggcgtgcgc gatcagggcc atgtcgcgga tgtggaagta ctccgcgcac ggcacccggg
      721 tggtgacgcg gtcgtcgtgt tccgggtcgt ccttccactc ctcgagccac tcctcgtacg
      781 gcgactccag cccgcgcgcc accatcgcct cgtgcaacgc ctcgatgcgc ggccggttga
      841 aactgtggtg gtagtagagc ttcaacggct gccacggctc gcccgcctcg ggatacgcgg
      901 tcgggtcagc ggccgccttc cacgcgtgca acgtcacctg atggcacatg atgtggtccg
      961 gatgagggta gccaccgttc tcgtcgtacg tcgtcacgac gtgcggccgg aagctccgca
     1021 tcaaccgcac cagcggctct gcagcctcct cgacgggcac cagcgcgaaa cacccgtcgg
     1081 gcaacggcgg cttcgggtcg ccctccggcc agccggagtc gacgaagccg agccagtcct
     1141 gggtgactcc caggatctcc ctcgcccgat ccatctcctg acggcggatc tccgtgatgt
     1201 tggcgagcac gtccggccgg tccatgttcg ggttgaggat cgagccgcgc tcgccaccgg
     1261 tacacgtcac gacgtggacg tcgacaccct cggcgacata cttggcggtg ctcgccgccc
     1321 ccttgctgga ctcgtcgtca gggtgcgcat gcacgtgcat cagccgtaga ccgttcgtca
     1381 actccatacc tggttctccg aaatccatcc cgacagctct tgatcccgac atgctcgatc
     1441 agcgaggtgg ggcatcgaac catcgtggca gggacgccca cgaggccgcg acctgcattt
     1501 ctggcactcc ccctccacca ctgccaacca ccaggaggga agatcattcc cgtgacctcc
     1561 cccggtgacg ctcgtccacc tggcgccgcg cccagccggc agccgctcca ggaacgctac
     1621 ggcgtacgga gtccgcgtga ccggcgcgtc ctgcaggtga gcgtggccgc cgtggccctc
     1681 gcgttcctcg cctggctggg ctgggctgcg tgggatcaca gccaccccga cgtgaccggg
     1741 cagctgcacg ggtacgacgt cgtgtccccg caccaggtcc gggtcgtcat cgacatcacg
     1801 cgcacggacg gtcagcacgt cgtctgtgac gtgctggctc aggccgagga ccactcagcg
     1861 gtcggggaga cgcaggtggc ggtccgggcc ggcggcgcgg acacggtgca ggtctccgcc
     1921 gtgatccgga ccgaccggga agccacgacc gcgaccgtgt cgaactgccg cgccgcggac
     1981 tgagggaccg cggtcccgtc cgtcgcgggt ccggttcgcc cgcggtcacc ttcacctacg
     2041 ttgggggtcc gggcccgatc tttgctaatc tcggattttc cccatcgggg catgcgacgg
     2101 agtcaagcag tccaagctca gttccgtgcg cccgcaacca aggagcacaa cccgtgacac
     2161 agcccgcgac tgagaaggcc atctggctga cgcaagaggc ttacgacggc ctcgcggccg
     2221 agctggagca cctcaagggg ccggctcgcg ccgagatctc gcaacggatc gcggacgccc
     2281 gggaagaggg cgatctgcgt gagaacggcg gctaccatgc cgcgcgcgag gagcagagca
     2341 agaccgaggc acggattctc cagctcgagg acatgctgag ccgcgccaaa gtcggtcaga
     2401 cacccgatga gggagacggg accatcgccg ccggtatgga ggtcaccgtg cggttcacgg
     2461 acggtgacac cgagaggttc ctgctcggcg cacgcgaggt gctggcacac gacaccgtcg
     2521 acgtcagcgt gtactcaccg cagtcgccgc tgggtgccgc gatcgtcggc aagtcggccg
     2581 gtgactcggc gacctacgcc gccccgaacg gcaacaaggt caccgtcgag atcgtcgagg
     2641 cgaagccctt caccggctga cgtcgccggc tgagatccgc tggttgcgcg tgtcggcgcc
     2701 aacggatctc cgttgggtcg gagacgtcag aagaccttga cgtcgtaccc ccgctcccgc
     2761 agcttggcga gcacgagatc gcggtgctcc tgaccgcgca tctccatctg caggacgacg
     2821 tcgacctggt cgagcggcag cgactccgag ttgcgctcgt ggacgacgtc gagcacgttc
     2881 gcgtcgcacg cgctcagctc cgacaacagc ttgaccaggc cacccggccg atcggagata
     2941 cggcactcga gcgccaggta gcggccctgt gcggccatac cgtgccggat cacgcgcatg
     3001 agcagcagcg ggtcgatgtt cccgccggac agcacggcga cgaccggacc ctcgaagtcc
     3061 tccgggcggt cgagcagcgc cgcgacggcg gccgcgcccg ccggttcgac gaccaccttc
     3121 gcccgctcca tcagcgcgag cagggccgtg gacagcgact cctcggagac ggtccggatc
     3181 tcgtcgacgt acgtctggat cgccgcgaac ggcacctctc ccggacagcc gaccgcgatc
     3241 ccgtcggcca tcgtggccat actgctcagt ggcaccgggt gcccggcggc gagcgatgcg
     3301 gggtacgccg ctgccgactc ggcttgtacg ccgaccacgc gcacgtcggg ccgcagcgac
     3361 ttcgctgccg tggcgatacc ggccaggaag ccgccaccgc cggtcgggac gacgatggtc
     3421 ttcacctccg gcacctgctc gacgatctcc agaccgcacg tgccttgtcc ggcgacgatg
     3481 tcgacgtggt cgaacgggtg gatcagcgcc gaccccgtct gcgcctggaa acgatgggcc
     3541 tcgatgagac agctgtcgat cgcgtggccg acgaactcca cgtcggcgcc gtagctgcgg
     3601 gtcgccttct ccttgggaat cggcgccccg atcggcatga acaccgtcga cttgatgccg
     3661 agcagcgagg ccgccaaggc caccccttgg gcgtggttgc ccgcactcgc cgcgaccacc
     3721 ccgcgtgcac gctcctcctc ggagaggtgg ctgatccgca ggtaggcgcc gcgaatcttg
     3781 aacgagccgg tgcgctgcag gttctcgcac ttcagccaga cgtcctgacc ggtgagcgcg
     3841 cgcagccagc gcgatccctc catcggcgtc accagcgcga cacctcgtag ctcctcgcgg
     3901 gcggcctgga tctgttcgag tgtgaccttc ggcacctccg ccgtcgaagc cgccggtcca
     3961 gactcctcgg tgtccatggg tgaagcgtag gcccagccga ccgcgcggca gcgggaagga
     4021 cattcagttg tatgcggcat gcatctagat gtatgcttgc gccatgcaag caaaaccttt
     4081 cgacctcgaa cagccgcagt cgcccgaaga ggcggtgatc gggctgctca tgcaggccgg
     4141 ccgacggctg cgtacgcgcc atcccgagga ccaggtcgac ccttcgacgt tccccctggc
     4201 caaacagctc atgtgccacg acggcatgcg gctgtcggac ctggcagcca aggtcgagct
     4261 cgacgcctcc acggtgagcc gccagatcaa gcagctcgag gacaagggca tcgtcgagcg
     4321 cacgccggac ccggctgacg gccgggccag cctcgtccgc ctgaccgacg agggtcatcg
     4381 cacgatccag gccggcttcc gacgccggtt cgagcgcatc cgaagcgccc tcgagccgtg
     4441 gagccagcag gaccgtgacg tgctgcagaa actcctgacc cgcctcgccg cagacctccg
     4501 caacgccaac gacctccacg aaagcgcgca ctccgcatga cgcagacacc acagacgcac
     4561 accgctgacg accacggtta cctcagccac cgacagattc tgatcgtgct gggcggcctg
     4621 atggccggca tgttcctcgc cgcgctcgac cagtcgatcg tcggcacggc cctcccccgg
     4681 atcaccagtg agctcggcgg cctcgccaag ttgtcctggg tcgtcacggc gtacctgctc
     4741 acccagacgg catccacgcc gctgtggggc aagatctccg acctgtacgg ccgcaagctg
     4801 atcttccaga cggcgatcat cacgttcatc ttcggctcgc tcatgtccgg gctggcgcag
     4861 aacatcggcg agctcatcgc gttccgcgcg gtgcagggac tcggcggcgg cgggctgttc
     4921 gcgctcgccc tggccacgat cggcgacatc gtcccgccgc gtgagcgcgg tcggtacggc
     4981 ggctacttcg gcgccgtgtt cggcacctcg tcggtgctcg gcccagtcct cggtggcttc
     5041 ttcgccgacg gacccggctg gcgctggatc ttcttcatca acgtgccgat cggcctggtc
     5101 gcgctggtga tcaccagcgc ggcgctcagg atccccgtcg tacgccggga gcacgccatc
     5161 gactacgtcg gcgccgcgac gatcgtggcc gcggtcagct ccctcctgct gtacaccacg
     5221 tgggccggtc cggaccacgg ttgggcctcg gggctcggtc tgaccctcct gggtgcaggt
     5281 ttcgtcctga ctggcctgtt cgtgtacgtc gagtcgcggg ccgccgagcc gatcctgccg
     5341 ctggacctgt tccgcatctc ggtgttccgg acggcgaacc tctacagctt catcatcggt
     5401 ttcgcgatgt tcggcggcat catcttcctg ccggtctatc tccaggtcgt gcagggcatg
     5461 agcccgaccg agtccggtct cgcgctgctg ccgatggtcg tcgggatctt ctcgacgtcg
     5521 atctcatccg ggatcgtgat gagcaggacc gggcgctacc ggctgttccc gatcatcggg
     5581 gccgtcaccg tgatcgccgc gctcgtcatg ctgacgaggc tcgacgtcgg cagtcactac
     5641 tggttcgcgg ccatcggctt ctacatcttc ggcgccggcc tcgggttcag catgcagatc
     5701 atcgtgacga tcgtgcagaa cgccgtggac cgtcggcaca tgggctcggc caccagcgcc
     5761 gtcgcgttct tccgctcgat ggggggaacg ttcggtgccg ccgtcttcgg tgcgatcctg
     5821 tcgagccggc ttgcggcgca cctcgcggac gagttcgccg gccagggcgg aggtgcagcg
     5881 ctgcccgccg gcggcgatgt caccaacaac gtcgaacgga tccagcacct gcccgaaccg
     5941 atccacggcc tggtgctgag ggcgttcgcc gactcgctgc acgacgtgtt cctctgggcc
     6001 attccggtcg tcgtggtcgc gctgttggtg gcgttcttca tcaaggagct cccgctgaag
     6061 atgcgggaga cgtcggagtt gtcagaaccc cattgagtcg taggtctacg ggtttctgac
     6121 aactcggttg aggagcaggt cgtacgccgc ggtcgggtgt tcggaggcga gcaacccggc
     6181 cgcggcgagc acgtagtcga ggtcgaccac cacacgtggc gatcggggga cgagccaacc
     6241 accggccacg tggtctcccg tacctgccgc caggatccgc gccgcgacgt cggctgcgtt
     6301 gtggcggagc acgccgcccg accccaccaa cagcccgacc tcacgcaggt ccttcccgct
     6361 tcgctcgacc agtcgaccgt ccgggctgaa gaccacctgc tgacgaccgg cgtggcggcg
     6421 tacggcgagt tggacggcgg ccgaagcgat cgccacgtcg tcctcgagct ctgtcggcgt
     6481 cacgggaatc atgctcgggt cggttcggcg tactttcgct gccgccgcca cgtcgtcagc
     6541 caggtcgagg ccggccgcac gggcggcctc cacggtcggg acggcgctcc agcgcatccc
     6601 gaggtcaccc tcgaccgtgc ggctgaccgg cacggtctcc acgacttcgc gacccagccc
     6661 ggcctcctcc gggtccacct caaccacgga gtgcacgtcg gtggtcgcgc cgccgacgtc
     6721 gaccacgacg acgtcaccga ctcccggccg ttccggtccg aggccggatc ccagcagctc
     6781 gacggcggtg agtacgacgt cgggggtcgc gcccttgacc atcgcggtga agtctgtgcg
     6841 cttcgacagg tgcttgccgc cgatcacgtg ccgcaggaac atcttccgga tcgcgccgcg
     6901 tgcgccttcc ggggccagct tcccgatctg cgggacgacg ttggccgaca cgacgtacgg
     6961 cactccggcg gcgtcgaaca gcgctgcgac atcgtcactc acatcggtgt tcccggcgag
     7021 caccaccggt gccgcccagt tggccaccgc cagtgagcgg gcattccgcc gcaacacgtc
     7081 ggcattgccg ccgtcggtgc cgccgacgag gagtacgacg tcgggacgac ttcgccgcag
     7141 cgcccgcagg tcgacctcgt cgtacgacac gtggacgacc cgaccgccac tcgacaacgc
     7201 gacgcgccgg cccgcctccg cggtcacgag ctcctcgttg ccgacaacgc cgatacgcag
     7261 tccgccgccg gccgacgaac aggcgaggac cggcacgtcc gcgccatccg tcagcggtgc
     7321 cagcctggcc cggatcacgt cccagccgtc caggacgtcg gtgtggatcg tcgtcgggtg
     7381 gtcgtcggtc gccaccaagc ggcccgcgtc gacgtcgacg agagccgctt tggtgaacgt
     7441 cgacccgaag tcgacgcaga agaccagcga catgaccaga cgaaaccgtc aaccgcgtcc
     7501 gacgagagcc tgctcgagat ccgcgaccag gtcttccacg gtctccagcc ccaccgacag
     7561 ccggacgagg tcgtcaggca cctcgagttc ggagccggag gccgaggcat gcgtcatcgt
     7621 ggccggcagc tcgatcagcg actcgactcc accgagggac tcggccagcg tgaacacgcg
     7681 tgcccggttg cacacctcca gggccgcggc gttcccggcg tcgacccgga aggacaccat
     7741 cccgccgaag cgcttcatct gggcggcggc cacctggtga cccgggtggt cgacgagccc
     7801 cggataaaac acgtcgccga cagcgtcatg gcggacgagg aagtcgacga tgcgctcggt
     7861 gttgtcgcag tgccggtcca tccggaccgg aagggtcttc agcccccgca gcacgagata
     7921 ggcgtcgaag ggtcccgccg tcgcaccggt ggcgttctgg aacagcgcaa gctcggcgcc
     7981 gagcgcctcg tcggccgtca ccacggcacc accgaccaca tcgctgtgcc caccggcgta
     8041 cttggtggtc gagtgcacga cgacgtcagc gcccagtgtg agcggccgtt ggagatacgg
     8101 cgtggcgaac gtgttgtcga cgacgaggat cgcatcgctg tcatgcgcga tctgggccag
     8161 cgccgggatg tcggcgacac cgagcagcgg gttggacggg gtctcgatcc agatgaccct
     8221 ggtacgaccg ggtcggaccg cggcggcgat tgctgccgtg tcgtgcacgg aggcggttgt
     8281 gtggtcgagc ccccagggtt cgagaacctg cttgaagagc cggtacgtcc cgccgtacgc
     8341 gtcgttcggg atgacgacgt gatcaccggg cgagcagacg acccgcagca acgcgtcctc
     8401 tgcagccatg ccggaggcga acgccacccc acgggtgccc tgctcgaggg cggccaggca
     8461 ctcctccaag gccgtgcggg tcgggttcgc ggtccgcgag tactcgtagc catcccgtag
     8521 gccgccgacg ccgtcctgct tgtaggtgct gacggcgtag atcggcgtca tcaccgcgcc
     8581 gttgttcggg tcggctcgct ggccggcgtg gatcgccttc gtctcgaacc cgtacgcgct
     8641 gctcatgcgc cgaagcctac gtggacttcc agggtccttc tggatcgagg cgccgcagca
     8701 cagcgacgac gtcgggcttg tccagcccac ccagatcggc gatctcgtcc cagccgatcc
     8761 aggcatgccc gaggtacgac gcgtgctcga tctcggtctg cccggagacg tctgggtcgt
     8821 cggccgacgc ggctcggccg aggaagaaga actcgtcgca cacgaaccgt tccccgagcc
     8881 agaagaggtc gcggccgacc acaacgggtt caccgacaag gcccggcgct gccaagccag
     8941 tctcctcggc ccactcgcgg agtaccgcgg tgcgcgggtc ctcgccttcc tcgatcccgc
     9001 ccccgggcgg ctcccagagc tcgaacccgt cgtacggatc ccgccagtgc aggagcaaca
     9061 cccggttgcg tgggtcgcag cagagcagcc gggcggcagg tcgtggctcc gcgggtgtcg
     9121 ccaccaggtc gatgacgtag tggacgatgt cctcgccgtt gctgtcgacg gctgccctac
     9181 gagaccaggt gaagccgagc gactcgtaca ggtgccgcgc gccggcgttg tcggagtgga
     9241 cgaacaactg cgcccgcgca aagcctcgac gcttcccctg cgacaggatc gcctgcacca
     9301 ctcgacggcc gaaaccacgt ccccacgacc ccggcctggt cgcgaccgag gagatgtgca
     9361 gcagtccagg cacctgccga ggactgcggc cgtcgtcagc gcgagcaggc atcgccactg
     9421 cgatcgcgag cagcatcgag cgttcgacca gcccgacgcc gaacgcgccc gggcgggaca
     9481 acacgtcggc gtcggtctcg tcaaggggag gcaacccgac ggcttcgcga cgggcggcgg
     9541 tggcggcacg ccacagctcg gagagctggc gacgatgcgc ctccgggtcg agctgcacga
     9601 cccggaagtt ctgaccgtca tcgaccacgt ccatacccaa gcacaaacca cggtccagca
     9661 gcttcgtgca aggcaggcgc aacagcccgg agaccgcctg cgcacgagct gatgcgacgg
     9721 atgccgtgcc acgatgagcg gatgagcagt cccgccggca gagcagtcga tcccgacagg
     9781 gtgcaggtcc tcaaggagcg cgaggaccga gcattcgtgg cggcccgccc gcgaagtgtg
     9841 ctgctcggaa agcaggcgct cggcgtgatg ccgaacggcg tccccatgag ctggctgcgg
     9901 acgtcgtacg accacccgcc gctgttcgtc gaccaggcat ccggctcgcg gttccgcgat
     9961 gtcgacggcc acgagtacgc ggacttcaac atcgcggaca tgtccatgtt cacggggtac
    10021 ggcccgcctg ccgtcgtcga cgcggtcagc cggcggatgg ctgccggatc gcagttcctg
    10081 ctgccgacgg aggactccat cgtcgtcgcc ggtgagctcg gtcgacgcta cgacctgccg
    10141 ttgtggcagt tcaccctcgc tgcgaccagc gccaacacgg aggcgatccg gatcgcgcga
    10201 gtggccaccg ggcgcgagtc ggtgctgttc ttcgacggca agtaccacgg tcacttcgac
    10261 gaggcgctcg tcgaagtcgt ggacggacga gtgcagccgg aggaggcagg actgccgtac
    10321 gacgtcacca cccgcacggt cgtcgttccg ttcaacgacc ccgacctgct cgagcgggcg
    10381 cttgcgtcgc gtcaggtcgc cctcgtcatc accgaaccgg cgctcaccaa caacgtcggg
    10441 ctgctgctgc cagagggcga ttttcatgtc cgactgcggg agctcacctc ctcgaccggg
    10501 accctgctgg cgtacgacga gacgcacaca caggtggtcg gcccgggtgg gctcacgagg
    10561 cgctggggcc tccagccgga catcgtcacg atcgggaagt cgatcgcctc cggcgtcccg
    10621 ctgggtgcgt acggcatgac cgaagaggtc gccgatgtgt tgcagcgccc tggcggtcgc
    10681 gatgacgaca agccgcaggt ggcggtcggc ggcacgttgt tcggcaacgc tttgtcgatg
    10741 gcggccgccc gcgccaccat gtcggaggtg ctcaccgacg acgcctacga gcacacacag
    10801 gtcctcggcg cccggctcgc tgacggcttg tccgatctca tcgcctctgc ccggctgcct
    10861 tggacggtgc accgcttctg gccacgcagc gggttgacgt tcgccagtgc catgccacgc
    10921 aacgcggtcg aggcgactgc ctcgttcgac gtaccgctcc ggcggctcat gcgggtgtat
    10981 ctcgcgaacc gccacgtgtg ggacgccatc gtcggagcgg gtccgacgtg ttcggtggcc
    11041 gcgacaaccg acgacgtcga ccagtacctg gccgcgatgg ccgagttgtt gagcgagctt
    11101 gtggcgtgac accgtccgac gtccgacctg ggtctcctca ggttgctgtg ggcgtgggcc
    11161 acgggtgggt ggtcgtccgg tgctccgttg gcttcgcctg aggtgcttgg cggcgaagag
    11221 cgggcgatgt tcctgcccgc tgagacctgt cggccggctt tcccttcctt gtgggcgggt
    11281 gtgggctggc gggtttcgga catccgccgg tcagcgggcc cgatctgcct tggtcccgtt
    11341 gcaaacccac ccctcccaag gcagacctgc ctggctaccc gtccgattgc atggctaccg
    11401 aggcaaacca accggaatac ggtgccccga aggggtgttc gcactcgtgc cacgtcgagg
    11461 agaggactgc cgatgatctt cgggcgcaag accgagatgc ccacccgcga caacgcgctt
    11521 cccggccgcg acacgcggtc gttcggcgtc gg
//
LOCUS       DATBFZ010000288         9209 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_4133219, whole genome shotgun sequence.
ACCESSION   DATBFZ010000288 DATBFZ010000000
VERSION     DATBFZ010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 9209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9209
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4133219"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /locus_tag="VH419_08885"
     CDS             <1..513
                     /locus_tag="VH419_08885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043768.1"
                     /translation="ELVKGKTSSRPMAILRGLAHLVLDPGDHGSGAAELIRASDLDLF
                     GLGTREAAVAAALRDDDVALAHFPPRVEVDPPPFSGVVSHDSDVRIDVRSLTDPLGWR
                     VDIRTQHAADPSALLYAGRLWERCSVVAAAHRLTAGQCASDDVTATEQVTKQTGNGWH
                     TLVSACWFLT"
     gene            555..1322
                     /locus_tag="VH419_08890"
     CDS             555..1322
                     /locus_tag="VH419_08890"
                     /inference="COORDINATES: protein motif:HMM:NF022747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3105 domain-containing protein"
                     /protein_id="HEY7043769.1"
                     /translation="MSKQNKNRERREMVEQMRRQARAAERRRTMVIFAICVVVALIIT
                     GAAIYTVVKNNREQDRLAGEDLASIGEAAQAAGCTSIKEQDASGQGQHTTEKINYAVV
                     PPAFGPHNPTPDQSGKHFFTADDRPDVEVLVHNEEHGWTIVWYDDSIAGNGSQMDLLQ
                     ATADKFDSAGSDFNAHVIIAPWTKDDGNGRPIPDNKHIAFTHWSIHQPNFDPKKFKQD
                     PSWGESQYCDTFSGAALNTFMQKFPYDDAPEGSLWHQ"
     gene            complement(1344..2342)
                     /locus_tag="VH419_08895"
     CDS             complement(1344..2342)
                     /locus_tag="VH419_08895"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HEY7043770.1"
                     /translation="MEAVIVAGGFGSRLRPLTDHLPKHLLPVAGEPFVAHQIAKLADA
                     GVRRVVLATSYYADRFEPVLGDGSAYGIELEYVMEDEPLGTGGAIRNAATALRGDDEP
                     VLILNGDILSGHDLEAQLAHHRRRGADVTLHLVEVDDARPYGCVPTDQEDRVTAFLEK
                     SADPVSRQVNAGCYLFDPEVVAAIPAGEVVSVERDTFPDLLADGWHVSGYLQQAYWLD
                     VGTPAALCRASADIVTGVVSTPLYDTGPAESWVHRTAVVAPDADLRAGTTIGPRARVE
                     AGVVAEASVLSEGVVVESGASLHHSVLGRGVHVGARAVLRDAALGDETEVSAAPSA"
     gene            3150..4157
                     /gene="galE"
                     /locus_tag="VH419_08900"
     CDS             3150..4157
                     /gene="galE"
                     /locus_tag="VH419_08900"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016668661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEY7043771.1"
                     /translation="MTVLVTGGAGYIGAHVVRLLRDRGDIVVVDDLSTGAAARVGDVP
                     LVQVDIAEDQAVDKLVALIQEYAVDSVVHIAAKKQVGESAERPAYYYRQNVGGMANLL
                     LAMEATHVDRLMFSSSAATYGLPEVPTGQNLTEDVPGRPISPYGETKLVCEWMCADAA
                     SAWGLRWAGLRYFNVAGSGWPDLGDPEALNLIPIVLTAVTNGRDPQIYGDDYDTPDGT
                     GIRDYVHILDLAQAHVDTLDYLERDDRPHEIFNVGTGRGSSVKEVISQIARTTGYDIQ
                     PQVVGRRPGDPAHLVADPARIADTLGWTSTRTLEDMIASAWEAWPSAHGTPVVTPPRT
                     G"
     gene            complement(4266..5000)
                     /locus_tag="VH419_08905"
     CDS             complement(4266..5000)
                     /locus_tag="VH419_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017934819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY7043772.1"
                     /translation="MPEAVIVDGVTKRFKLANQKTFKRLTVNAVNRRQLHRSLTALNE
                     VSFTLEEGDSIGLMGLNGSGKSTLLKLISGVMAPDEGKVWTRGRVAGLIEVGAGFHPD
                     LTGRENVYLNAAILGMSEEETNAKFDSIVAFSEMGNFLGNEVRHYSSGMFMRLAFAVA
                     IHTECDIFLMDEMLAVGDQPFKRKCMRRIKELRESGKTMIYVSHSPKQVLNLCNRGLV
                     LEQGKMVFEGSAQDAVRKIGYDQDED"
     gene            complement(5000..5911)
                     /locus_tag="VH419_08910"
     CDS             complement(5000..5911)
                     /locus_tag="VH419_08910"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY7043773.1"
                     /translation="MTVGTRPAAPAAGDGPLLPLVNPGRVGGLRDVFRRRYLLKLLVR
                     KELKVRYAGSSVGLAWSYVKPAVRFAMYYWVIGLLLGNRLENRALHIFAGMVLVSFTT
                     ATLNAGCKSVVKNGSLIKKINMPREMFPVASVLVSGYYMIPMYVILLLGCMIAGWHPD
                     LGALLAAVMALALVLIWGLGIALFLSAWNVFFRDTANVVDVLQTLVTWTVPMIYPFAI
                     VMDKLQGRHELIYQLYLANPLCVGVLLNNRAFWIPASDDPAALAAMDLPEHLYERGAI
                     AILAGLVFVWVGQTVFSRFETKFAEKL"
     gene            complement(5908..7092)
                     /gene="glf"
                     /locus_tag="VH419_08915"
     CDS             complement(5908..7092)
                     /gene="glf"
                     /locus_tag="VH419_08915"
                     /EC_number="5.4.99.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013159983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008767 - UDP-galactopyranose mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009273 - peptidoglycan-based cell wall
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
                     /protein_id="HEY7043774.1"
                     /translation="MNAGDADIVVVGSGLFGLTVAERCANDLGLRVRVLDRRSHVGGN
                     AYSAAEPETGIEVHKYGAHLFHTSNERVWEYVNRFTTFTNYQHRVFSIYQGRVYPMPI
                     NLGTICEYFGRSMTPDEARALVADQAAEVDTTTASNLEEKAISLIGRPLYEAFIRGYT
                     AKQWQTDPKKLSADIISRLPVRYNFDNRYFSDTYEGLPVDGYTAWLERMVDHPLIDVH
                     LETDFFDVRDAYIGSVPIVYTGPVDAYFDNAAGELSWRTVDLDLSVKDIGDFQGTSVM
                     NYADEDVPFTRIHEFRHFHPERDWYPKDKTVIVHEYSRFADKGDEPYYPINTPEDRDK
                     LLKYRELAKAEPNVLFGGRLGTYKYLDMHMAIGSALSMYDNKLRPFFADGVSLESGGV
                     DA"
     gene            complement(7243..8220)
                     /locus_tag="VH419_08920"
     CDS             complement(7243..8220)
                     /locus_tag="VH419_08920"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY7043775.1"
                     /translation="MTPSDPPPVQAPPNCFIVAVTYNRAEYLARLLESIDAMSPRPAG
                     LVVVDNASTDGTAEVIETYERSVPEGFVHVRRFEENVGGSGGFSAGVETALEQGAEWV
                     WTMDDDVEVLPEALLLLGRWTGRFGCLHGRRWDHDGTPFFWQTRMNSWLGIFYPRPGD
                     VFEGRDYFLTNSGTFEGMMIRRDVVEQIGLPDPRFFLTWDDAVYAWLASQITEVAYVN
                     AFVLRRLRPQRSISLGIRHLNDSSDLSRYYVMRNRGYVANYLAAHDRYRPVGFALGTF
                     LTFGKEMLRSVAVEHSVKGIAPILRGYRASRALRRDPSWRPMPPLTEQD"
     gene            complement(8217..9143)
                     /locus_tag="VH419_08925"
     CDS             complement(8217..9143)
                     /locus_tag="VH419_08925"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY7043776.1"
                     /translation="MTAIQEPVVAAAILTHNRPADLAVLLERLGKQTRVVDTTVIVDN
                     GTQACDLTPFEAELDIRYVKSEANLGGAGGFALAMLTALSANADWVWVMDDDARPESE
                     TCLQALLDEADARRLSAISPVIVAPEDASKLAFPFRVDKKFTYDRGFVEQTPFWPQQA
                     LLFNGLLIRREAVLEVGLPDLKMFIRGDEVDYLLRLRESGLSFGTSTAAAFVHPTGWA
                     EVSSIVEGRFHVLVPETELKRFYFFRNRGYLIRRHKRPVSLLVDVIGYSVHYLVKERD
                     RAGFRAWRQAFWTGLRSDFSGPDRSGGGTETS"
BASE COUNT         1643 a         3080 c         3035 g         1451 t
ORIGIN      
        1 gagctggtga aggggaagac gagcagccgg ccgatggcga tcctgcgcgg gctcgcccac
       61 ctcgtcctgg atcccggtga ccacggctcg ggtgccgccg agctgatccg cgcgagcgac
      121 ctcgacctgt tcggcctcgg gacacgcgaa gctgctgtcg ccgcggcact tcgtgacgac
      181 gacgtcgcgc tcgcgcactt cccgccccgc gtcgaagtgg acccgccgcc gttctccgga
      241 gtcgtcagcc acgacagcga cgtacggatc gacgtccgat cgttgacgga tcccctcgga
      301 tggcgcgtcg acatccgcac gcagcacgcg gcggacccgt cggccctgtt gtacgccggt
      361 cggctctggg aacgttgctc ggtggtggcc gccgcccatc gcctcacggc cggccagtgc
      421 gcgtccgacg atgtcacagc cacggagcag gtgacgaaac agaccggtaa cggctggcac
      481 acgctggtca gcgcctgctg gttcctcacc tagactgtgc ctccgttgtt ggcaccagag
      541 aggccgaagc acccgtgagc aagcagaaca agaaccgcga acgtcgcgag atggtcgagc
      601 agatgcggcg gcaggcgcgg gcagccgaac gccgccgcac gatggtcatc ttcgcgatct
      661 gtgtcgtggt ggccttgatc atcaccgggg cggccatcta caccgtcgtc aagaacaacc
      721 gcgagcagga tcgactcgcg ggcgaagacc tcgccagcat cggcgaagca gcccaggccg
      781 ccggttgcac ctcgatcaag gagcaggacg cctccgggca gggacagcac acgacggaga
      841 agatcaacta cgcggtggtg ccaccggcgt tcggaccgca caacccgaca cccgaccagt
      901 ccgggaagca cttcttcacc gccgacgacc gtccggacgt cgaggtcctg gtccacaacg
      961 aagagcacgg ctggacgatc gtctggtacg acgactccat cgccggcaac ggcagccaga
     1021 tggacctgct ccaggcgacc gccgacaagt tcgacagcgc cggcagcgac ttcaacgccc
     1081 acgtgatcat cgctccgtgg acgaaggacg acggaaatgg ccggccgatc cccgacaaca
     1141 agcacatcgc cttcacgcac tggtccatcc accagccgaa cttcgacccg aagaagttca
     1201 agcaggaccc gtcctggggc gagagtcagt actgcgacac gttctccggc gccgcactga
     1261 acacgttcat gcagaagttc ccgtacgacg atgcgcctga gggaagcctc tggcaccagt
     1321 agccggcggt tcgagcggcc cgttcacgca gacggggccg ccgacacctc cgtctcgtca
     1381 ccgagtgccg catcccggag cacggcacgc gcgccgacgt ggacgccgcg gccaaggacg
     1441 ctgtggtgga gactcgcacc ggactcgacc accacgcctt cggagagcac cgacgcctca
     1501 gcgaccacgc cggcctccac ccgagcgcgc ggccctatcg tcgtaccggc ccgcaggtcg
     1561 gcgtcgggag ccacgacggc cgtacggtgc acccaggact cggccgggcc ggtgtcgtag
     1621 agcggtgtcg agacaacacc cgtcacgatg tcggcagagg cgcgacacag cgcagccggc
     1681 gtacccacgt ccaaccagta cgcctgctgg agatagccgg agacgtgcca gccgtcggcc
     1741 agcagatcag gaaacgtgtc ccgttcgacg gacacgacct caccggcggg aattgcagcg
     1801 accacctcgg ggtcgaacaa gtagcagccg gcgttgacct ggcgcgagac cgggtcggcg
     1861 gacttctcga ggaaggcggt gacccggtcc tcctggtcgg tcgggacaca tccatagggc
     1921 cgcgcgtcgt cgacctcgac gagatgcagc gtcacgtcgg caccgcggcg ccgatgatgc
     1981 gccagctggg cctccaagtc gtggccgctg aggatgtcgc cgttcaggat caggaccggc
     2041 tcgtcatcgc cgcgtagcgc ggtggccgcg ttgcgaatgg ctccgcccgt gcccagcggc
     2101 tcgtcctcca tgacgtactc cagctcgatg ccgtacgccg agccgtcacc caggaccggc
     2161 tcgaaccggt ctgcgtagta gctggtcgcg agaaccacac gccgaactcc ggcgtccgcc
     2221 agcttggcga tctggtgcgc cacgaacggc tcgcccgcca ccggcagcag gtgcttcggg
     2281 aggtggtcgg tgagcggacg aaggcgcgaa ccgaaccctc cggcaacgat gacggcttcc
     2341 actcgttcat ccaatcagtg ccatcggctt tttcgatggc tccgcgccag gaccgcggcg
     2401 gagaaggtcg cgaggtgagc gtgatccccg gctgtcagct gtcacgggct gaggggcgtg
     2461 gcattgacgg caggagggtg gtttgcctgg tgagccatcc caaacggacg gggagccaga
     2521 ctgatctggc ctcttctgaa gtttgttgcg ggccgcgcct ggtaccaccc gcctccgaaa
     2581 agccacctag gccaccattc ggacagtccg ggctggaggt ttgcgatgtt tacagcccaa
     2641 aaggcgtgtc aacatcgcaa accttcgatg ggaaccgtgg gtctcggatg tcgacatcgc
     2701 aaacatcacg ccggtggtgg tttgcctcgg tagccatcca aatggacggg tcaccaggca
     2761 ggtctgcctg gggagcggtg ggtctgcctg gtgaccgagg cagatcgggc cagctgatcg
     2821 ccggatgtcc gaaacccgct agtcacacac cacgcaaaag gcacggaacg ccggccgacg
     2881 agctcaccgg gcacgatggt tcccgctctt cggtgccgac ccacctgagc tcaccacgac
     2941 gcaagcccgg cgaccacgcc cacccatcac gaccgattcc gccccacagg aacctgagga
     3001 gttctccatg cgatgtcgtt tgatgatcga ggtgtcgatc ggcgtccgac acgccggcaa
     3061 aacccaacca aacctgccac ctcggcggta ggcaggacgg ggagcgggcg acgtccggtc
     3121 cgggaggggc cgcggataca gtgtcggcca tgactgtcct ggtgaccggt ggcgcaggct
     3181 acatcggtgc ccatgtcgtg cgcctgctgc gcgaccgcgg cgacatcgtg gtcgtggacg
     3241 acctcagcac cggtgcggcg gcccgggtcg gcgacgtgcc gctcgtgcag gtcgacatcg
     3301 ccgaggacca ggccgtcgac aagctcgttg cgctcatcca ggagtacgcc gtcgactcgg
     3361 tcgtccacat cgcggcgaag aagcaggtcg gtgagtcggc cgaacgtccc gcgtactact
     3421 accgccagaa cgtcggcgga atggccaacc tgttgctggc catggaggcg acgcacgtcg
     3481 accggctgat gttctcgtcg tcggctgcga cgtacggcct gccggaggtt cccaccggac
     3541 agaacctcac cgaggacgtc ccgggcaggc cgatcagccc gtacggcgag acgaagctgg
     3601 tctgcgagtg gatgtgcgcc gatgccgcat ccgcctgggg cttgcgctgg gcgggtctgc
     3661 gatatttcaa cgtcgccgga tcgggctggc cggatctcgg cgaccccgag gcgctcaacc
     3721 tgatcccgat cgtgctgaca gcggtgacca acggccgtga cccgcagatc tacggggacg
     3781 actacgacac cccggacggc accggcatcc gcgactacgt tcacatcctc gacctcgcgc
     3841 aggcccacgt ggacacgctc gactacctcg agcgcgacga caggccgcac gagatcttca
     3901 acgtcggcac cggacgtggc tcgtcggtga aggaagtgat ctcgcagatc gcgcggacca
     3961 ccgggtacga catccaaccg caggtggtcg gccgacggcc gggtgatccg gcgcacctcg
     4021 tcgccgaccc ggcccggatc gctgacaccc tgggttggac gtcgacgcgg acgctcgaag
     4081 acatgatcgc gtcggcctgg gaggcctggc cctccgcaca cggcactccg gtcgtcaccc
     4141 cgccccgcac cggctgaccg agctgaccga gcgcccaagg cacccgcgga ttgcgggaaa
     4201 cgggcggata ttccggggtg tcggggctcc actttcccgc aattcgcggg gaccaggcag
     4261 tgcggtcagt cttcgtcctg gtcgtagccg atcttgcgga ccgcgtcctg ggcgctgccc
     4321 tcgaacacca ttttcccctg ctcgagcacc agcccgcggt tgcacaagtt caacacctgc
     4381 ttggggctgt ggctgacgta gatcatcgtc ttgccgctct cgcgcagctc cttgatccgt
     4441 cgcatgcact tgcgcttgaa gggctggtcg ccgaccgcga gcatctcgtc catcaagaag
     4501 atgtcgcact cggtgtggat cgcgaccgcg aaggcgagcc gcatgaacat gccggacgag
     4561 tagtgccgga cctcgttacc gaggaagttc cccatctcgg aaaaggcgac gatcgagtcg
     4621 aacttcgcgt tcgtctcctc ctcgctcatc ccgaggatgg ctgcgttcag gtagacgttc
     4681 tcgcgaccgg tgaggtccgg atggaaccca gcgccgacct cgatgaggcc agcgacccgg
     4741 ccacgggtcc acaccttgcc ctcgtccggc gccatcaccc cggagatgag cttgagaagg
     4801 gtgctcttcc ccgacccgtt gaggcccatc aggccgatgc tgtcgccctc ttcgagggtg
     4861 aacgagacct cgttgagcgc ggtcagggac cggtgcagct gacgacggtt cacggcgttg
     4921 accgtcaacc tcttgaaggt cttctggttc gcgagcttga aacgcttcgt cacgccgtcg
     4981 acgatcacgg cttccggcat cagagcttct ccgcgaactt ggtctcgaac cggctgaaca
     5041 ccgtctgccc aacccagacg aacaccagcc cggccaggat ggcgatggca ccgcgttcgt
     5101 acagatgctc tggcaggtcc atggccgcca gggcggccgg gtcgtcggac gcggggatcc
     5161 agaaggctcg gttgttcagc aagacgccca cgcacagcgg gttggcgagg tacagctgat
     5221 agatcagctc gtggcgcccc tggagcttgt ccatcacgat ggcgaacggg tagatcatcg
     5281 gcacggtcca ggtgaccaag gtctggagca cgtccacgac gttcgcggtg tcccggaaga
     5341 acacgttcca ggcactcagg aacagcgcga tacccagacc ccagatcagt accagcgcga
     5401 gcgccatcac cgcagccagc agcgcaccga ggtccgggtg ccaaccggcg atcatgcagc
     5461 cgagcagcaa gatcacgtac atcgggatca tgtagtaccc cgataccagc acggacgcga
     5521 cggggaacat ctcccgcggc atgttgatct tcttgatgag cgagccgttc ttgaccacgg
     5581 acttgcagcc ggcgttgagg gtggcggtcg tgaacgagac cagcaccatg ccggcgaaga
     5641 tgtggagtgc ccggttctcg agcctgttgc cgagaagcag accgatcacc cagtagtaca
     5701 tggcgaagcg aaccgccggc ttgacgtacg accaggccag accgaccgac gatccggcat
     5761 agcggacctt cagctccttg cgcaccagca gcttgaggag gtaccgacgg cggaacacgt
     5821 cgcgcaggcc gccgacccga ccggggttga ccagtggcag cagcggtccg tcacccgctg
     5881 caggcgctgc cggcctggtg ccgacggtca cgcgtcgact cctccgctct ccaatgacac
     5941 cccgtcggcg aagaacggcc ggagcttgtt gtcgtacatc gacagcgcgg agccgatcgc
     6001 catgtgcatg tcgaggtact tgtacgtgcc gagccgtcct ccgaagagca cgttcggctc
     6061 ggccttggca agctcccggt acttcagcag cttgtcccgg tcctcgggcg tgttgatcgg
     6121 gtagtagggc tcgtcgccct tgtccgcgaa gcgggagtac tcgtggacga tgacggtctt
     6181 gtccttcggg taccagtcgc gctcgggatg gaagtgccgg aactcgtgga tccgggtgaa
     6241 cgggacgtcc tcgtcggcgt agttcatcac tgaggtgccc tggaagtcgc cgatgtcctt
     6301 gacgctgagg tccaggtcga cggtgcgcca ggaaagctcg ccggcggcgt tgtcgaagta
     6361 cgcgtcgacc ggaccggtgt agacgatcgg gacgctgccg atgtacgcat cgcgcacgtc
     6421 gaagaaatcc gtctcgagat ggacgtcgat cagaggatgg tcgaccatcc gctcgagcca
     6481 ggccgtgtag ccgtcgacgg gcaggccctc gtaggtgtcg ctgaagtagc ggttgtcgaa
     6541 gttgtagcga acggggagcc ggctgatgat gtccgccgac agcttcttcg ggtcggtctg
     6601 ccactgcttg gcggtgtagc cccggatgaa ggcctcgtac agcggccggc cgatcagcga
     6661 gatggccttc tcctcgaggt tggacgccgt cgtggtgtcg acctcggcgg cctggtccgc
     6721 gacgagggct cgggcttcgt ccggcgtcat cgacctgccg aagtactcgc agatcgtgcc
     6781 caggttgatc ggcatggggt agacgcggcc ctggtagatc gagaagaccc gatgctggta
     6841 gttggtgaag gtcgtgaacc ggttgacgta ctcccagacc cgctcgttgg acgtgtggaa
     6901 caggtgggcg ccgtacttgt gcacctcgat gccggtctcg ggctccgccg cgctgtaggc
     6961 gttgccgccg acgtgtgagc gccggtcgag cacgcggaca cgcaggccga ggtcgttcgc
     7021 gcaccgttcg gcgacggtga gaccgaacag tccagatccg accacgacga tgtcggcgtc
     7081 gccagcgttc acacacactc ccggggatgg gcgttcacaa cacactcggg cccaacgcac
     7141 tcgtcaaggg gcccgagcaa gtctacgggg atcaaggcgg cgttccggcc agccccgccc
     7201 ggcaggtgac gatcttcacg cccgctgtga gtacgccgtc cttcagtcct gctcggtcag
     7261 cggcggcatc gggcgccacg acgggtcgcg gcgcagcgcc cgactggcgc gatacccacg
     7321 caggatcggt gcgatcccct tgaccgagtg ctcgaccgcc accgacctca gcatctcctt
     7381 gccgaacgtc aagaacgtgc cgagcgcgaa gccgaccgga cggtaccggt cgtgggcggc
     7441 gaggtagttg gccacgtagc cgcggttgcg catgacgtag tagcgggaca ggtcgctgga
     7501 gtcgttgaga tgccggatgc ccaagctgat gctccgctgg gggcgcagcc ggcgcaggac
     7561 gaacgcgttc acataggcga cctccgtgat ctgtgaggcc agccaggcgt acaccgcgtc
     7621 gtcccaggtg aggaagaacc gcgggtcggg caggccgatc tgctcgacga cgtcgcgacg
     7681 gatcatcatc ccctcgaacg tgccgctgtt ggtcaagaag tagtcccgcc cctcgaacac
     7741 gtcgcccgga cgcggataga agatgcccag ccagctgttc atgcgggtct gccagaagaa
     7801 cggcgtaccg tcgtggtccc agcgtcgccc gtgcagacaa ccgaaccttc cggtccagcg
     7861 gcccaacaac aacagcgcct ccggcagcac ctcgacgtcg tcgtccatgg tccacaccca
     7921 ctcggcgccc tgctcgagcg ccgtctcgac gccggcggag aagcctccgg atccgccgac
     7981 gttctcctcg aaccgccgca cgtgcacgaa gccttccggg accgagcgct cgtacgtctc
     8041 gatcacctcc gccgtcccgt cggtgctggc gttgtcgacg acgaccagac cagccggtct
     8101 gggtgacatc gcgtcgatgc tctcgagcaa cctggccagg tactccgcac ggttgtacgt
     8161 caccgccacg atgaagcagt tcggcggagc ctggacgggt ggcgggtcgg acggggtcat
     8221 gacgtctccg tgccgccgcc ggatcggtcg ggcccgctga aatcggatcg caggcctgtc
     8281 cagaacgcct gccgccacgc ccgaaagccg gcgcggtcgc gttccttcac caagtagtgg
     8341 acggagtagc cgatcacgtc gaccagcagg ctgaccggtc gtttgtgccg gcggatgagg
     8401 taaccgcggt tccggaagaa gtagaagcgc ttgagctcgg tctccggaac gaggacgtgg
     8461 aagcggccct cgacgatgga ggacacctcg gcccaaccgg tcgggtggac gaacgcggcc
     8521 gccgtggacg tgccgaagga gagccctgac tcgcgcagcc ggagcagata gtcgacctcg
     8581 tcgccgcgga tgaacatctt caggtccggc agcccgacct cgagaaccgc ctcgcgacgg
     8641 atcagcagac cgttgaagag cagcgcctgc tggggccaga acggcgtctg ctccacgaac
     8701 ccgcggtcat aggtgaactt cttgtcgaca cggaaaggga acgccagctt cgaggcatcc
     8761 tcgggcgcga cgatgaccgg cgagatcgca ctcaaccggc gggcgtccgc ttcatccagc
     8821 aacgcctgaa ggcaggtctc cgactccgga cgggcatcgt cgtccatcac ccagacccag
     8881 tcggcgttcg cggacagcgc cgtcagcatc gcaagcgcaa acccgccggc gccgccgagg
     8941 ttcgcctcgg acttcacgta gcggatgtcg agctcggcct cgaacggggt caggtcgcag
     9001 gcctgcgtgc cgttgtcgac gatgacggtc gtgtcgacca ctcgtgtctg cttgccgaga
     9061 cgctcgagca ggacagcgag gtccgcgggt cggttgtgcg tgaggatcgc ggcggcgaca
     9121 acgggctcct ggatggccgt caccctcgct cacaccttcc gtctgagtgc atggcgcgtc
     9181 gacagtacgg ccctgccgac ggcggagtc
//
LOCUS       DATBFZ010000289         5332 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_5679096, whole genome shotgun sequence.
ACCESSION   DATBFZ010000289 DATBFZ010000000
VERSION     DATBFZ010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 5332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5332
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5679096"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            160..1176
                     /locus_tag="VH419_08930"
     CDS             160..1176
                     /locus_tag="VH419_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEY7043777.1"
                     /translation="MDPVEALERITYLLDRDLAPAVKASAFQRAAETVRATDPAELRT
                     LHDEGRLQELPGIGESTGKAIAEALEGKTPTYLQRLEETTTIEVGEGGDIRAALKGDC
                     HTHSVWSDGGAPIEKMALAAQDLGHEYMVLTDHSARLTVAHGLSAERLLEQLDEVDEL
                     NEKLAPFRILTGMEVDILEDGTLDMTDEMLSRLDVVVASVHSKLRMERSEMTRRMVLA
                     MASPHTDILGHCTGRKVVGRGRPESQFDPEIVFAACARFDKAVEINCRPERQDPPEEL
                     LTMALDWGCKVAIDTDAHAPGQLEWQPYGCDKAARLGIETGEIVNTMEADDLIAWAAS
                     HPSE"
     gene            1298..2431
                     /locus_tag="VH419_08935"
                     /pseudo
     CDS             1298..2431
                     /locus_tag="VH419_08935"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2639..3310
                     /locus_tag="VH419_08940"
     CDS             2639..3310
                     /locus_tag="VH419_08940"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEY7043778.1"
                     /translation="MSPAFSPVIASFVEDGGCYAVACIRGGNEEGELWHRAGMREHKQ
                     QVFDDFHAAADWLVQQGVTTRTQLAIRGGSNGGLLMGAAITQRPDLCRAVVCAVPLLD
                     MVRYHEFLIARLWIPEYGDPDVAEEFAWLYAYSPYHRVVDGTCYPAVLIETGEDDSRV
                     DPLHARKFAARLQAATSCGSEHPILVRIESKAGHGQGKPATRQADEAADVLAFVWWQL
                     SDAET"
     gene            complement(3565..5130)
                     /locus_tag="VH419_08945"
     CDS             complement(3565..5130)
                     /locus_tag="VH419_08945"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY7043779.1"
                     /translation="MSDLSPSTTNAVPLELPTGAVLQAGRFVVQRTLGRGGFGITYAA
                     HDHRLQRPVALKELFCDGAVRQGGVVTPPSHGVEAFAAAKARFLREASVLARFTHPGI
                     VRVYEVFEEAGTAYLVMELLEGRTLFDHVLKRGGPLTESDTLEVAQRCGDALASVHAS
                     GILHRDLNPANVMLAGSGRVVLIDFGLAREYAVDETTPMTRMVTPGYAPPEQYALNAR
                     SGPPADVYGLAATMYWMLTARAPTPAIDRQTGTPLTPPYRIIDTVSKTVSDGVLDGLE
                     LNPGHRPQTVDAFLARVGCSPVAANGQTQSSAPSPDHNVTRAEGTRPLPEPPAPLPVP
                     SAVEPGRRKALLPLFVIALAFGALIPIISVAVVAVLVLPALATAGDAVVYVRLRRSGD
                     RLLWRHRAALPGYIPLRFVRNLGHVLMAGIPALLLAGATVATALAVDSVTSTSTAEDW
                     VLRIGGAGAMAALVLLVVRDRLQFRVALILDRLVSYAVNDGKLTPVGQGLWIVAGVFL
                     VGAVGLRPELYPF"
BASE COUNT          910 a         1769 c         1828 g          825 t
ORIGIN      
        1 gctccatctc ggtcgagggt agtgggactg ttcgatcgct cggcttcgag cgctcgctgc
       61 gctcgctgtc tccgccgagt gcgcctcgtc gctggcgctt ccctcggcgc ctgatcgctc
      121 ggcttcgagc gctctcaacc gcgtctgtac ctttgcggcg tggacccggt cgaggcgctc
      181 gaacgcatca cgtatctgct ggaccgtgac ttggcgcccg ccgtcaaggc cagcgcgttc
      241 cagcgggccg ccgaaaccgt tcgggccacc gacccggcgg agttgcgcac cttgcacgac
      301 gagggccggc tacaggaact gccgggcatc ggcgagagca ccggcaaggc catcgcggag
      361 gccctggagg gcaagacgcc tacgtatctt caacggctcg aagagacgac caccatcgag
      421 gtcggcgagg gcggcgacat ccgcgctgcc ttgaagggcg actgccacac ccattcggtg
      481 tggtccgacg gcggcgcgcc gatcgagaag atggccctcg ccgcgcagga tctcggccac
      541 gagtacatgg tgctgaccga ccactcggct cgcctgacgg tcgcgcacgg cctgtccgct
      601 gaacggctgc ttgagcagct cgacgaggtc gacgagctca acgagaagct cgcgccgttc
      661 cgcattctca cgggtatgga agtcgacatc ctcgaggacg gcaccctcga catgaccgac
      721 gagatgctga gccggctcga cgtcgtggtg gccagcgtcc attcgaagct ccggatggaa
      781 cgttcggaga tgacgcgtcg catggtgctg gccatggcca gtccccacac cgacatcctc
      841 ggtcactgca ccggacgcaa ggtcgtgggc cggggacgtc cagagagcca gttcgatccc
      901 gagatcgtgt tcgcggcgtg tgcccgcttc gacaaagccg tcgagatcaa ctgccgtccc
      961 gagcgccagg acccgccaga ggagctgctg accatggcgc tcgactgggg atgcaaggtc
     1021 gcgatcgaca ccgacgcgca cgcccccggc cagctcgaat ggcagcccta cgggtgcgac
     1081 aaggcagcgc gtctgggcat cgaaaccggc gagatcgtca acacgatgga ggcggacgat
     1141 ctcatcgcct gggcggcgtc acaccccagc gagtaacgtc ttctctccat gtcgaaacgg
     1201 accgtgaagc ggaagaacag ggcgcgtcgc aagaaggcca accacggacg caagcccaac
     1261 gccggccgcg gctgacccgt cacctgcccg ggtcagcgac cacgtcgacc tgatccacgg
     1321 cgtcgaggtc gcggatccct accgctggct cgaggacgcc gaaagcgaag agaccaaggc
     1381 gtgggcggcg taccagaacg cccgcacacg gggcttcctc gacacgattt cgcggcgagc
     1441 gcagctgcat gctcgactca ctgagctctt ccgtgcaggc acgtcgtcgg cgccggtgat
     1501 cgaggcgggg caggtcttct ccctcgacag gtggggccac cacgatcggg cggtgctgtg
     1561 cgtacgaccg ctggcgggat cagggccgta cgagccgacc gccgtgctcg atcccatggc
     1621 ggtcgtgttc gacgagacgg cagccatcga ctggttccat ccatctcgtg acggcacgct
     1681 gatcgcatac ggcatctccg cgtcgggcga cgagcggtcg acgctgcgag tcctcgatgt
     1741 gcagaccggc gagagcctgt ccgacgagat ccccaacacg cgagcagcgt cggtcggctg
     1801 gctaccagac gcctccggct tcgcctacac ccgctacgcg acaggcggcg actacgaccg
     1861 ccacgtcttc tggcaccggc tcggcacggc gtggaccgaa gacccggtgc tgttcgacga
     1921 ccttcccgat ccgaccgcgt ggccggacgt gtcgatctcg ctggacggtc gccacgcggt
     1981 cgtccacgtc gagctcggct ggagccgcaa cgacgtgcac gtgatcgacg tgtcgaccgg
     2041 cgacaggcga acggtgatcg aaggcgagga agtcgtgact tggctgtcgg tggatgaccg
     2101 gcgggctcgg ctggtgggcc acaccactct cgacgccgat cgcggtcgtg tgatcgcggt
     2161 gccgctggtc ggcgaatcgc tgggtccgtc gcagtggcag acgctcgtgc cggagagcga
     2221 cgccgtgatc gaaggcacgg tgctgacgag tgactcactc ctcattgctt cgactcggcg
     2281 cgcggtcgcc cagctcgcgc atcatcgggc agacggggcc tacgtcggtg aagtgacgct
     2341 gccggagctc ggctcgttcg cgggtctcag cgcgagtcgc gacgaggacg ccgcggtgct
     2401 ctcgttcacg ggattcaccc ggccgcccac gctgttccga tggagcggcg acggctcggt
     2461 cgatcagtgg agcgaccttc cgggcgcgcc cgatgccggg gcgttcgccg tctcccagca
     2521 ttcgtacccg tcgacagacg gaacggaagt cgcggtgttc ctcgtgcgcg gtgccaaagt
     2581 cccagctgac tcgacgctcc cgaccatcct caccggctac ggcggctttg cgatcgggat
     2641 gtcgccggcg ttcagcccgg tcatcgcatc gttcgtcgag gacggtgggt gttatgccgt
     2701 cgcgtgcatc cgcggtggca acgaggaagg cgagctttgg caccgcgccg ggatgcgcga
     2761 gcacaagcag caggtgttcg acgacttcca cgccgccgcc gactggttgg tgcaacaggg
     2821 cgtcacgact cgcacgcaac tcgccatccg gggcggttcc aacggcgggc tgctgatggg
     2881 cgcggccatc acgcagcgcc cagatctgtg ccgagccgtc gtgtgtgccg tgccgctcct
     2941 cgacatggtg cgctaccacg agttcctcat cgctcgacta tggatccccg agtacggcga
     3001 tccggatgtc gccgaggagt tcgcgtggct ctacgcgtac tccccgtacc accgcgtcgt
     3061 cgatgggacg tgttaccccg cagtgctcat tgaaacgggc gaggacgaca gtcgcgtgga
     3121 tccattgcac gcccgcaagt tcgcggctcg tttgcaggcc gcaacgtcct gtgggagcga
     3181 gcatccgatc ctcgtccgca tcgagtcgaa ggccggtcac gggcagggca aacctgccac
     3241 ccgccaagcc gacgaagccg cggacgttct cgccttcgtc tggtggcagc tgtcggacgc
     3301 cgagacctag agcttcgccg accgagccgc ccgcgccctc gtcacgcaca ttgtcgagtg
     3361 agcatggaat tccgcaggct cggtcgacac agagtcgagg tcgtggatct ctgtgtcgac
     3421 acagcctcga aaacgcaggc tcggtcgaca cagagtcgcg tgcgtgtctg tgttgactca
     3481 gccacctcaa atcgaggctg gctcgacaat gtgcggccgg ggccgggggg gaagaaggct
     3541 gcagtccggg agacgacgcg agacctagaa ggggtagagc tcggggcgga gtccgacggc
     3601 gccgacgagg aagacgccgg cgacgatcca cagtccctgg ccgacgggcg tgagctttcc
     3661 gtcgttgacg gcgtaactga cgagccgatc caggatcagc gcgacgcgaa actgcagcct
     3721 gtcccggacc accagcagca ccaatgccgc catcgcaccg gcgccaccga tacggagcac
     3781 ccagtcttct gcggtcgagg tcgacgtgac gctgtcaaca gcgagggcgg ttgccaccgt
     3841 ggcacctgcg aggagcagcg cggggatccc cgccatgagg acgtgaccga ggttgcggac
     3901 gaaccgcagc gggatatagc cgggcagcgc agcgcggtga cgccacaaca agcggtcacc
     3961 cgaacgtcga agccggacgt acacgacggc atcccctgcc gtcgcgagcg caggaagaac
     4021 aagcaccgcg accaccgcca cgctgatgat cggaatcagc gctccgaacg cgagcgcgat
     4081 cacgaagaga ggaagcaagg ccttgcgccg tccgggttcg acggccgaag gaaccggcag
     4141 tggcgccggc ggttcgggca ggggacgagt cccttccgcc cgcgtcacgt tgtgatccgg
     4201 cgatggcgca gacgactggg tctgcccgtt cgctgccacc gggctgcatc cgacgcgcgc
     4261 gaggaacgcg tcgacggtct gcggtcgatg tccgggattc aactcgagcc cgtcgagcac
     4321 accgtcggac accgtcttcg aaaccgtgtc gatgatccgg tagggcggcg tgagtggtgt
     4381 ccccgtctga cgatcgattg cgggcgtcgg cgcacgcgcc gtcaacatcc agtacatcgt
     4441 tgcagcgagc ccgtagacgt cggccggagg cccgctgcgg gcattgagcg cgtactgctc
     4501 gggcggggcg taccccggcg tcaccatcct cgtcatgggt gtggtctcgt cgaccgcgta
     4561 ctcgcgggcg agaccgaaat cgatgaggac cactcgtccg ctgcccgcga gcatcacgtt
     4621 cgccgggttc aagtcgcgat gaaggatgcc ggacgcgtgc actgatgcga gagcgtcgcc
     4681 acagcgttgc gccacctcga gcgtgtcgga ttccgtgagg gggcccccgc gcttcaagac
     4741 gtgatcgaag agcgtgcgtc cctccagcaa ctccatcacg aggtatgcag tgccggcttc
     4801 ctcgaacacc tcgtacaccc ggacgatccc cggatgcgtg aagcgggcga ggacggatgc
     4861 ctctcgcagg aaacgggcct ttgcagccgc gaaggcttcg actccgtgcg acgggggcgt
     4921 tacgacgccg ccctggcgga ccgccccgtc acagaagagc tccttgaggg cgaccggccg
     4981 ctggaggcgg tgatcgtggg cggcataggt gatgccgaag ccaccgcggc cgagggtgcg
     5041 ctgcacgacg aaccggccgg cttgcaggac ggcacccgtg ggcagctcga gggggactgc
     5101 gttcgtcgtc gaaggagaga gatcactcat cgccggctgc gatcacggta gctgtacggt
     5161 cagggccatg ggggcgccga ccatcgacac gatcgtgatc gccgatccgc cggagacctg
     5221 gtcagcggcc gggttcacgg tcgacgacga agggatctgc cgggtcggca cggtccgaat
     5281 ccgtctcgcc ggacccgaag cgggggcgcg gatcgtctct tggacgctgc gt
//
LOCUS       DATBFZ010000290         8337 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_2491660, whole genome shotgun sequence.
ACCESSION   DATBFZ010000290 DATBFZ010000000
VERSION     DATBFZ010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 8337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8337
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2491660"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..461
                     /locus_tag="VH419_08950"
     CDS             <1..461
                     /locus_tag="VH419_08950"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEY7043780.1"
                     /translation="EQPARRHPRVDPRRPAGRPSLNEYVVDASAAILALVGTGRGSAD
                     LRGRLPRMRCHAPHLLDAEVGNVLRRHEQSGRISSEEAQEALSAIHAVVRLRYPHTGE
                     LAAAAWALRANLTFYDALYVALAGLLDVPLLTCDKRLSRVRRLPCHVELV"
     gene            complement(466..3921)
                     /locus_tag="VH419_08955"
     CDS             complement(466..3921)
                     /locus_tag="VH419_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012922185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEY7043781.1"
                     /translation="MTELLDAVARRRITEDTAATLFVNAGAGSGKTSALVDRIATLVL
                     RDGVPLNAIAAVTFTEKAGAELRDRLRATFEAARRSSVVLDLDKLDQRTRADEALDDL
                     DAAAIGTLHAFAQRILMEHPIEAGLPPLLEVLDEVGSSVAFEDRWSELQTDLLEDDDI
                     AETLLLGMACGVQTKHVRSLALLLGSDWDLIEDRVLIDDESPLTALDVSGLAEEAARI
                     AAMASQCRDPEDRLAARLPELEEFGAFLRAARDDSERIAVLDSIKKFKVGRTGNSKNW
                     PSVQAVRDACAELVEQASRLVAEVLDQCLRTLTRWTARKVLAGAEGRRAEGTLEFHDL
                     LVLARNLLRKNADVRAALHDRYQRLLLDEFQDTDPIQIELAVRIAGGEAADAPDWRDV
                     EVPPGRIFVVGDPKQSIYRFRRASIATYLDAEQYLGDTVSLTTNFRTGPDVLDWVNGV
                     FGDLISFEEGGQPAYEALARHRPAAPTGPPVTMLGTAAHDDLPRAAASILREREAADV
                     AAVIRRASAEQWQVHDERADGWRPVRHDDVAILVPARTSLPFLEDALDEAGVPYRAEA
                     SSLVYQSDEVRSLLACARAVADPSDALSLVTALRSPAFGCGDDDLWRWKHAGGSFSIY
                     SRLEDESPAPPVLDLDKLDQRTSAVERSPAVELVETPVASGPVGRALAYLRRLSFDAR
                     WMTPSEVLGAIIADRRMLEIAATGPRERDTWRRLRFVVDQARAWSEVSHGGLRAYLAW
                     AAHQGQETTRVAEAVLPETDTDAVKVMTIHAAKGLEFPIVILSGMTSESRKPSGVQLL
                     WPSEGGYAVKLTAEIQTGDFEAAYPVDEQMDGLEKRRLLYVAATRARDHLVVSLHRNG
                     VTRKSTAAQLLTDAGAVTPTVAQAPPIEEMPPVEPFETNGLGTIAPPPEWESWLAELT
                     EVRDRSRLVSAISASGLEGTEPEIVLAAPDVLDLDKLDQRTPAVEPFDGAQEPPVETP
                     DHPGRAKGPRDVELPPWSKGRYGSAIGRAVHGVLQVINLATANGLDDAVAAQSLAEGV
                     TDQADLVRALVMSALDSDVVQRAATRDHWRETYVATVHDDGTILEGFVDLVYRDDDGS
                     LVIVDYKTDAVPAGAIDSRVEYYRPQLRAYVDAHAAAQPDPVRAELLFLHPAGAVRAE
                     VANSS"
     gene            complement(3918..7028)
                     /locus_tag="VH419_08960"
     CDS             complement(3918..7028)
                     /locus_tag="VH419_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012922184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEY7043782.1"
                     /translation="MAVQLVTTSYGRPALEELRRVVADAKADDPMRTVTIIVPNNLAG
                     IVARRHLAHGLADGSPGVAALHITTLSRLAEQLVAHRLTPRRPATGPIVSAAWRKVLR
                     EEPGVFEEVADHPATVRALASAHRELRDVSSAGREKIREVTTITPDLVRLHEQVVANL
                     EAEWYDATDLLLAAADLVAGDAGPSELGHLVLYLPQDLDQAEASFATSLAARSALIVI
                     AGLTGVRRADAAVERTLARLGLEPPAPPTIATAHEILNASDSDDEVRCVVRDLVETLK
                     RAPAHRVAVLYAATSPYARLLHEHLAAAQLNVNGSGTRAVDERAVARVLLDVLALADG
                     DVPRAEMFRALSNAPTRTFTGERIPISRWERISREAGVVSGDDWTTRLDGLVDRERAA
                     IRTEESNDDPIQWRIERSRRTIDTANELKEFATALRHELGRAAAMTRWAELGAWCLDV
                     FTTLLGEGNDLLRLPPEEQYAAATVTTLLRGLGGLDTVDESANFQGLRELLALELAQA
                     LPRVGRFGEGVLVAPISHAIGLDLDAVYVLGLSEDLYPGRLHEDPLLPQRVRVASGGE
                     LPSYRERLHTRHRHLLAAFAAAPRCVASFPRGDLRRSSRRLPSRWLLPSLRELSDDKQ
                     LAASDWEKADLQGHLTTSGSYAGELLTTTTLAHEQEWRVRTARAGRDLDDDIVRAGVE
                     MVRSRHSARFTRFDGNLTAVEGLPDYAHGERVISPTALEGYAECPHAFLVQRLLGVEP
                     LEQPEDIVEISPADVGNIVHESLDRLVTAYAGKLPGHGEPWSDEQRAGLVTIAEETMR
                     VFEERGLTGHPRLWEREKVRILADLGWLIDDDNEWRAENGARVEASELTFGMRGRPAV
                     EVPVPGGRMRMRGSADKVDVGADGTIYVTDVKTGSRRTFRDIKQEDPFVDGTKLQLPV
                     YAYAARTRFGDPATPVHTAYWFVRPKRTDPGNRCRIGFELTPEIETEYAATLATLTSS
                     IHAGLFPLRAPDQPDFAWVQCHYCNPDGIGHAANREAWERKRHDPVLRNLVALLEPDV
                     HEEVDS"
     gene            complement(7131..7469)
                     /locus_tag="VH419_08965"
     CDS             complement(7131..7469)
                     /locus_tag="VH419_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017935106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase family protein"
                     /protein_id="HEY7043783.1"
                     /translation="MRMRTLLSSALVGAAVVAPTAVTAPAANAATMHTWNRLAHCESS
                     GRWHINTGNGYYGGLQISPSTWRAYHGKRFAPLPHQATKRQQIWVAERIKRGQGWRAW
                     PVCSQRIGVR"
     regulatory      complement(7472..7653)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            complement(7845..>8337)
                     /locus_tag="VH419_08970"
     CDS             complement(7845..>8337)
                     /locus_tag="VH419_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043784.1"
                     /translation="CDALIEGRNEGWVEVDKQVEWLSAYVEKCMVEAWGDHAEELGRH
                     GDVVTYRAGTASGRVQVECETPVIVRVMAKAVTGVRPTAKLLREINRVNACSRIAHLW
                     WHHGDVVVECPLYAEAVTPDSLRETCFHVSAVANDIGVVFAALFDGATPFPPLANDSE
                     DAA"
BASE COUNT         1220 a         2850 c         2932 g         1335 t
ORIGIN      
        1 gcgagcaacc ggcccggcgc cacccgcgag tcgatcctcg ccgacctgcg ggcagaccgt
       61 cgttgaacga gtacgtcgtc gacgcctccg cggcgatcct cgccctggtc gggaccggcc
      121 gtggttcggc ggacttgcgc ggacgactgc cgaggatgcg ttgtcacgca ccgcatctgc
      181 tcgatgcgga agtgggcaac gtgctccgtc ggcatgaaca gtcgggtcgg atcagttcgg
      241 aggaggcgca ggaagccctg tccgcgatcc acgccgtcgt acgccttcgc tatccgcaca
      301 ctggcgaact cgcagcggcg gcgtgggcac tgcgcgccaa cctgaccttc tacgacgcct
      361 tgtacgtcgc gctcgccggc ctcctcgacg tgccactcct gacctgcgac aagcgcctct
      421 cccgggtgcg tcgactcccc tgccacgtcg aactcgtctg actgctcacg acgagttggc
      481 cacctcggct cgcacggcgc cggcgggatg gaggaagagc agttcggcgc ggacggggtc
      541 gggttgggcg gcggcatggg cgtcgacgta cgcccggagc tgcggacggt agtactcgac
      601 ccgcgagtcg atcgcgccgg ccgggacggc gtcggtcttg tagtccacga tgacgagaga
      661 gccgtcgtcg tcgcggtaga cgaggtcgac gaagccttcg aggatggtgc cgtcgtcgtg
      721 gacggtggcg acgtaggtct cgcgccagtg gtcacgggtg gcggcgcgct ggacgacgtc
      781 ggagtcgagg gctgacatca cgagagcgcg caccaggtcg gcttggtcgg tgacaccttc
      841 ggcgagtgac tgggcggcga cggcgtcgtc gagcccgttc gcggttgcca ggttgatcac
      901 ctggagcacg ccgtggacgg cccggccgat cgctgagcca taacggccct tggaccaagg
      961 tggcagctcg acgtcgcggg ggccttttgc gcggccggga tggtccgggg tctcgacagg
     1021 tggttcctga gctccgtcga agggctcgac cgccggagtc cgctgatcga gcttgtcgag
     1081 atcgaggacg tccggagcgg caagcacgat ctcgggttcg gtgccctcca gaccggaggc
     1141 gctgatcgct gagacaaggc gagaacgatc gcgcacctcg gtgagctcgg cgagccagga
     1201 ctcccactcg ggcggtggag cgatggttcc gagtccgttg gtctcgaagg gctcgaccgg
     1261 cggcatctcc tcgatcggcg gagcctgcgc gaccgtcggt gtgaccgcgc cggcgtccgt
     1321 cagcagctgg gctgccgtcg acttcctggt cacaccgttg cggtgcaggg agacgaccag
     1381 atggtcgcgg gcacgggtgg cggcgacata cagcagccgc cgcttctcga gtccgtccat
     1441 ctgctcgtcg acggggtagg cggcttcgaa gtcgccggtc tggatctcgg cggtgagctt
     1501 gacggcgtac ccgccctcgc tcggccagag cagctgcacg ccggagggct tgcgggactc
     1561 cgacgtcata ccggacaaga tgacgatcgg gaactccagc cccttcgcag cgtggatcgt
     1621 catcaccttg accgcatccg tatcggtctc gggcagcacc gcttcggcga cccgggtggt
     1681 ctcctgcccc tggtgggccg cccaggcgag gtacgcccgc aggccgccgt gcgagacctc
     1741 ggaccacgcc cgtgcctggt cgacgacgaa ccgcagccgg cgccaggtgt cacgttcgcg
     1801 cggcccggtc gcggcgatct cgagcatccg gcggtccgcg atgatcgcgc cgagcacctc
     1861 gctcggtgtc atccagcgcg cgtcgaagga aagccggcgc aagtaagcca gcgcccggcc
     1921 cacagggccg ctggccaccg gggtctcgac aagctcgacc gccggactcc gctcgaccgc
     1981 cgatgtccgc tgatcgagct tgtcgagatc aaggactggc ggagccggac tctcgtcctc
     2041 gagtctcgag tagatcgaga acgaaccgcc ggcgtgcttc caccgccaca ggtcgtcgtc
     2101 accgcagccg aacgccggcg accgcagcgc cgtcaccagc gacagcgcgt cgctggggtc
     2161 ggccacggct cgcgcacacg ccagcagact gcgcacctcg tcggactggt agaccagcga
     2221 cgacgcctcg gcccggtacg gcacaccggc ctcgtcgagc gcatcctcca ggaacggcag
     2281 cgatgttcgc gccgggacca ggatcgcgac gtcgtcgtgc cgcaccggcc gccagccgtc
     2341 ggcgcgctcg tcgtgcacct gccactgctc ggccgacgcc cgtcgaatga ccgcggccac
     2401 gtcggccgcc tcccgttcgc gcaggatcga cgccgcggcc cgcggcaggt cgtcgtgtgc
     2461 cgccgttccg agcatcgtca ccggtggacc ggtcggcgcg gcggggcggt gtcgcgcgag
     2521 ggcctcgtac gccggctgac ctccttcctc gaacgagatc aggtccccga agacgccgtt
     2581 gacccagtcg agcacgtcgg ggccggtgcg gaagttcgtg gtcagactga cggtgtcgcc
     2641 gagatactgc tcggcgtcga ggtacgtcgc gatgctcgcc cggcggaacc ggtagatcga
     2701 ctgcttcggg tcaccgacca cgaagatcct gcccgggggg acctcgacgt cacgccagtc
     2761 cggtgcgtcc gccgcttcgc cgccggcgat gcgtacggcc agctcgatct ggatcgggtc
     2821 agtgtcctgg aactcgtcga gcagcagccg ctggtaacga tcgtgcaggg cagctcggac
     2881 gtcggcgttc ttccgcagca ggtttcgagc gaggacgagc aggtcatgga actccagcgt
     2941 cccttcggct cgtctgccct ctgcgccggc gagcaccttg cgcgccgtcc atctcgtcag
     3001 cgttcgcagg cactggtcga gcacctcggc gaccaggcgg ctcgcctgct cgaccagctc
     3061 agcacaggcg tcgcgcaccg cttgcacgct cggccagttc ttggagttgc cggtccgtcc
     3121 gaccttgaac ttcttgatgc tgtccaggac ggcgatgcgc tccgagtcgt ccctggccgc
     3181 tcgaaggaag gcgccgaact cctcgagctc gggaagccgg gccgcaagac ggtcctcagg
     3241 atccctgcac tggcttgcca tcgccgcgat ccgggcagcc tcctcggcga ggccggagac
     3301 gtcgagcgcc gtgagaggcg actcgtcgtc gatgagcaca cgatcctcga tgaggtccca
     3361 gtcggagccg aggaggagcg ccagcgaacg cacgtgtttg gtctgtacgc cgcacgccat
     3421 gccgagcagc agcgtctcag cgatgtcgtc gtcctcgagc aggtcggtct ggagctcgga
     3481 ccaacggtcc tcgaacgcca ctgacgagcc gacctcgtcg agcacctcga gcagcggtgg
     3541 cagtccggcc tcgatcgggt gctccatcaa gatccgctgg gcgaaggcgt gcagtgtgcc
     3601 gatggccgcc gcatcgaggt cgtcgagcgc ctcatccgcc cgtgtccgct gatcgagctt
     3661 gtcgagatcg aggaccaccg aagaccggcg ggcggcttcg aacgtggcgc ggagccgatc
     3721 gcggagctcg gcgccggcct tctcggtgaa cgtcacggcc gcaatcgcgt tcagcggtac
     3781 gccgtcgcgc aacacgagcg tggcgatccg gtcgaccagg gccgacgtct tgccagatcc
     3841 cgccccggcg ttcacgaaca acgtcgcggc ggtgtcttcg gtgatgcggc gacgcgcgac
     3901 agcgtcgagc agctcggtca cgagtccacc tcctcgtgta cgtcaggttc gaggagggcg
     3961 accaggttgc gaagcaccgg atcgtggcgc ttgcgttccc aggcttcccg gttggcggcg
     4021 tggccgatgc cgtccggatt gcagtagtgg cactgcaccc acgcgaagtc aggctggtcg
     4081 ggtgcccgca gcgggaacag tccagcgtgg atggagctgg tcagcgtggc cagcgtcgcg
     4141 gcgtactccg tctcgatctc cggcgtgagc tcgaagccga tccggcaacg gttgcctggg
     4201 tcggtgcgct tgggacggac gaaccagtac gccgtgtgga ccggcgtcgc cgggtcgccg
     4261 aaacgggtcc gcgcggcgta cgcgtacacc ggcagctgca gcttcgtgcc gtcgacgaac
     4321 gggtcttctt gcttgatgtc gcgaaacgtc cggcggctgc cggtcttgac gtcggtcacg
     4381 tagatcgtgc cgtcggcgcc gacgtccacc ttgtcggcgc tcccgcgcat ccgcatccgg
     4441 ccacccggca ccggcacctc gacggcgggc cgaccacgca tgccgaacgt cagctcgctc
     4501 gcctcgaccc gtgcgccgtt ctcggcccgc cactcgttgt cgtcgtcgat cagccagccg
     4561 aggtcggcga ggatgcggac cttctcgcgc tcccacagcc gcgggtgccc ggtcaggccg
     4621 cgttcctcga acacccgcat cgtctcctcg gcgatcgtca ccagaccggc gcgctgttcg
     4681 tcggaccacg gttcaccgtg tccggggagc ttgccggcgt acgccgtgac gagccggtcg
     4741 agggactcgt ggacgatgtt gccgacgtcg gccgggctga tctcgacgat gtcctcgggc
     4801 tgttcgagcg gctcgacacc gagcagtcgc tgcaccaaga acgcgtgcgg gcactcggcg
     4861 tacccctcta gggcggtcgg tgagatcaca cgttcgccgt gggcgtagtc cgggagacct
     4921 tcgacggccg tgaggttgcc gtcgaagcgg gtgaaccgcg cgctgtgccg cgaccggacc
     4981 atctcgacgc cggcccggac gatgtcgtcg tcgagatcgc gcccggctcg ggccgtccgg
     5041 actcgccatt cctgctcatg cgcgagcgtc gtcgtggtga gcagctcgcc ggcgtacgaa
     5101 ccggacgtcg tgaggtggcc ctgcaggtca gccttctccc agtcggacgc ggccagctgc
     5161 ttgtcgtcgc tgagctcgcg caggctcggc agcagccatc ggctcgggag gcggcggctc
     5221 gaccggcgca ggtcgccccg ggggaacgac gcgacacagc gtggtgcggc cgcgaaggcg
     5281 gcgagcaggt gccgatgcct ggtgtggagg cgctcgcggt acgacggcag ctcgcctccg
     5341 gacgcgactc ggactcgctg cggcagcagc ggatcctcgt gcagccggcc ggggtagagg
     5401 tcctcgctga gcccgaggac gtagaccgcg tccagatcca acccgatggc atgcgagatc
     5461 ggtgcgacca ggacgccttc gccgaacctg ccgacccgcg ggagcgcctg ggccagctcc
     5521 agggcgagca gctcccgcag tccctggaag ttcgcgctct cgtcgacggt gtccagcccg
     5581 ccgagaccgc gcagcaacgt cgtgacggtc gcggcggcgt actgctcctc aggtggcagc
     5641 cggagcaggt cgttgccctc accgagcaac gtagtgaaca cgtcgaggca ccacgcgccg
     5701 agctcagccc atcgggtcat ggcggctgca cgcccgagct catgacgcag agcggtcgcg
     5761 aactccttca gctcgttcgc cgtgtcgatg gtccgcctgc tgcgttcgat ccgccactgg
     5821 atcgggtcgt cgttggactc ctcggttctg atcgcggccc gctcacgatc gaccagaccg
     5881 tcgagccgtg tcgtccagtc gtcgccggac acgacaccgg cctcgcgaga gatgcgctcc
     5941 caccgcgaga tcgggatgcg ctcgccggtg aaggtccggg tgggcgcgtt cgacagcgcc
     6001 ctgaacatct ccgcacgcgg cacgtcaccg tcggcgagtg cgagtacgtc gaggagcacg
     6061 cgggcgaccg cgcgttcgtc gacggctcgg gtgccgctgc cgttgacgtt gagctgtgct
     6121 gcggcgaggt gctcgtgcag gaggcgggca tacggagacg tggcggcgta caacaccgcg
     6181 acccgatgcg cgggcgcccg cttcagcgtc tccaccaggt cgcggacgac gcagcggacc
     6241 tcgtcgtcgg agtcggaggc gttgaggatc tcgtgcgcgg tggcgatcgt cggtggcgcg
     6301 ggcggctcga gaccgagccg ggccagggtg cgttcgaccg cggcgtcagc tcggcgtact
     6361 ccggtgagtc ctgcgatgac gatcagcgct gaccgcgcgg caaggctggt ggcgaacgag
     6421 gcctcggcct ggtcgaggtc ctgcggcagg tagaggacca ggtgaccgag ctctgacggc
     6481 ccggcgtcgc cggcgacgag gtcggcagcg gccagcaaca ggtcggtggc gtcgtaccac
     6541 tcggcttcga ggttcgccac cacctgttcg tggagtcgga ccaggtcggg agtgatggtc
     6601 gtgacctcgc ggatcttctc gcgtcctgcg gagctcacat cacgcagctc gcgatgcgcg
     6661 ctcgccaacg cccggacggt ggccgggtgg tcggccacct cctcgaacac gccgggctcc
     6721 tcgcgcagta ccttgcgcca ggcggcggac acgatcggcc cggtcgcggg tcgacgcggc
     6781 gtcagccggt gcgcgaccag ctgctcggcc agccgggaga gtgtcgtgat gtgaagagcc
     6841 gcaacgccgg gactaccgtc ggcgagcccg tgcgcaagat gtcgacgagc gacgatgccg
     6901 gcgaggttgt tcggcacgat gatcgtgacc gtgcgcatcg ggtcgtcggc cttcgcgtcg
     6961 gccacgacac gacgaagctc ctcgagcgca gggcgaccgt acgaggtggt caccaactgc
     7021 acggccacgg tctacctctc ccgtcgaagg cgcgcccttt gtcaccgtag ccgcaaccga
     7081 gttgtcagaa gtacatggac ctatagccct atgtacttct gacaactcgg tcagcggacg
     7141 ccgatgcgtt gcgagcacac gggccacgcg cgccagccct ggccgcgctt gatgcgctcg
     7201 gcgacccaga tctgctgacg cttggtcgcc tggtgcggca gcggcgcgaa gcgcttgccg
     7261 tggtaggcgc gccaggtgct ggggctgatc tgcagtccgc cgtagtaccc gttgcccgtg
     7321 ttgatgtgcc agcggccact ggactcgcag tgggcgagcc ggttccatgt gtgcatggtg
     7381 gccgcgttgg cggccggggc ggtcacggcg gtcggggcaa caacggccgc gccgacgagg
     7441 gcagacgaaa ggagcgtgcg catgcgcatg atgagttcct ccacgcgcct gcggggttag
     7501 ctgtcgggtt cgggctcgaa ggtgcccggc catgctgagt ggcttcaccc cgagggccgc
     7561 tggcgcgacc cggagaacca gtgggttccc cgctcctgtc cgacgagctt gtcgatgggc
     7621 cttggcgcgg acaggactcg gcggacacgc aaggccgtcc cggctgatcg ggacgatcga
     7681 gagtaggcac gccatcgcgc cggcgtcgaa ccggtccggg actgctgttg cccttggggt
     7741 tggcggtgca tgagtcccct ggttttgtga ccggtgtctc gcggcctgtc ggcgtacctt
     7801 cacagcatgg gcgacacagc aggacgcacc gaactgaccg ctggtcaggc ggcgtcttct
     7861 gagtcgttgg cgagtggagg aaacggcgtc gccccgtcga acagcgcagc gaagaccacg
     7921 ccgatgtcgt tggcgaccgc gctcacgtgg aagcaggtct cgcgcaggct gtccggcgtg
     7981 accgcctccg cgtacaacgg gcactcgacg accacatcac cgtggtgcca ccacaggtgc
     8041 gcgatgcgtg agcacgcgtt gacgcggttg atctcgcgca gcagcttggc ggtcggtcgt
     8101 acgccggtca ccgccttcgc catcacgcgc acgatcaccg gcgtctcgca ctcgacctgc
     8161 acccgccccg atgcggtgcc tgcgcggtag gtgacgacgt cgccgtgtct tcccaactcc
     8221 tcggcgtgat ccccccacgc ctcgaccatg cacttctcga cgtatgccga gagccactcc
     8281 acctgcttgt ccacctcgac ccacccctcg tttcgaccct cgatgagagc atcgcat
//
LOCUS       DATBFZ010000291         3317 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_530405, whole genome shotgun sequence.
ACCESSION   DATBFZ010000291 DATBFZ010000000
VERSION     DATBFZ010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 3317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3317
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_530405"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..393)
                     /locus_tag="VH419_08975"
     CDS             complement(46..393)
                     /locus_tag="VH419_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY7043785.1"
                     /translation="MQEVLVKSLATIDACCAPLLAAPLDAGDAEELALAFKALADPVR
                     LQLLSLIAAAPAGTACSCDLEAPVGKSQPTVSHHLSVLADAGLISKEKVGRWVNCTVV
                     PERLATLRDVLGG"
     gene            457..909
                     /locus_tag="VH419_08980"
     CDS             457..909
                     /locus_tag="VH419_08980"
                     /inference="COORDINATES: protein motif:HMM:NF041414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsI/CadI family heavy metal resistance
                     metalloenzyme"
                     /protein_id="HEY7043786.1"
                     /translation="MSRVQLALNVPDIDDAVAFYSKLFATEPNKRKPGYANFAIADPP
                     LKLVLFEKAGADTRLNHLGVEVADSDAVWAHQQRLSDEGVDSIEESGTCCFAKQDKVW
                     VDGPDGSWEIYTVLADSDMFGQSSAKVEDQAQDTLCCASVPEAEARCC"
     gene            909..1916
                     /locus_tag="VH419_08985"
     CDS             909..1916
                     /locus_tag="VH419_08985"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEY7043787.1"
                     /translation="MPITSAPRAPITSPAVWRAIDVADPASWTIELSDNQRDEITAVA
                     RAAHREGRTVATLTLDEVALPALTETLNQLVETLSAGRGFVLVRGFPIEQLDQAETEL
                     AYAALGLHLGTPVSQDAHGTLLGHVRDERIQRTGPEVRLYRTRERQDFHTDGADIIGL
                     LCLHAAKAGGESKLASSYAVYNEILDRRPDLLEVLYEPMWWDRNGEESPGDDPAYPLP
                     VLHDVDGMPRIFYIGWYIRDAQRHPAVPRLTNAQRAALELIESIANDPAFHVVMDFER
                     GDVQLLNNAKILHSREAYEDDEDLDNRRHLLRLWLSARQFTSVEERLRGGIPQRHSKG
                     R"
     gene            2744..3205
                     /locus_tag="VH419_08990"
     CDS             2744..3205
                     /locus_tag="VH419_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEY7043788.1"
                     /translation="MPRTDRATRVIAAPLDRVFDALVDHDALLAWLPPRGMTARFERF
                     DPRPGGSYRLVLTYTDTADSRPKSSPDSDIVEARYVDIVANVRVVQAVDFMSGDPQFA
                     GTMTMTWAVAAVDGGTRVDITADDVPDGISADDHASGLTSSLENLATYIES"
BASE COUNT          604 a         1129 c         1058 g          526 t
ORIGIN      
        1 ctccgcggcc gtcaagcaaa caacgggtga acgagtatcg gcggatcagc cgccgagcac
       61 gtcgcgcagg gtggcgagcc gttcgggcac gacggtgcag ttgacccacc gcccgacctt
      121 ctccttgctg atcaaccccg cgtcggcgag cacggagagg tggtgcgaga cggtcggctg
      181 gctcttgcca accggcgcct cgagatcaca gctacaggcg gtgccagcgg gtgcggcggc
      241 gatgagcgac agaagctgca gccggaccgg gtcagccagc gccttgaagg cgagcgccag
      301 ctcttccgcg tcgccggcgt cgagcggcgc ggcgagcagt ggggcgcagc aggcgtcgat
      361 ggtggcgagt gacttcacga gaacctcttg cattcatcga caaatgtcga tacattgatg
      421 gctgtcaata gtttacgtga tctcggaggt ttcgcaatgt ctcgtgtcca gctcgccctc
      481 aacgtccccg acatcgacga cgccgtcgcc ttctactcca agctgttcgc cactgagccg
      541 aacaagcgca agcccggcta cgccaacttc gccatcgcgg acccgcccct caagctcgtg
      601 ctgttcgaga aggcgggcgc cgacacgcgg ctaaaccatc tcggtgtcga ggtcgccgac
      661 agtgacgccg tgtgggccca ccagcagcgt ctctcggacg agggtgtcga ctcgatcgag
      721 gagtccggca cgtgttgctt cgcgaagcag gacaaggtgt gggtcgacgg ccccgacggc
      781 tcctgggaga tctacaccgt gctcgccgac agcgacatgt tcggccaatc ctcggcaaag
      841 gtcgaggatc aggcccagga cacgctgtgc tgcgcgtcgg tgcccgaggc cgaagcgcgc
      901 tgctgctgat gccgatcacc agcgcgcccc gcgcgccgat cacgtcgccg gcggtgtggc
      961 gcgctatcga cgtcgccgat ccagcgtcgt ggacgatcga gctgagcgac aaccagcgcg
     1021 acgagatcac agcggtcgcg cgtgctgcgc accgtgaggg tcgcacggtt gcgaccctca
     1081 ccctcgacga ggtggcgctc ccggcgctga ccgagaccct caaccaactc gtcgagacgc
     1141 tgtcagcggg acgcgggttc gtgctcgtgc gcgggttccc gatcgaacag cttgaccaag
     1201 ccgagaccga gctcgcgtac gccgcgctcg gactgcacct cggcacaccg gtcagccagg
     1261 acgcgcacgg cacgctgctc ggccatgtcc gcgacgaacg catccagcgc accggacccg
     1321 aggttcgcct ctaccggacc cgcgagcggc aggacttcca caccgacggc gccgacatca
     1381 tcggcttgct gtgcctgcac gccgcgaagg cgggtggcga gagcaagctg gctagcagct
     1441 acgccgtcta caacgagatc ctcgaccgcc ggcccgatct actcgaggtc ctgtacgagc
     1501 cgatgtggtg ggaccgcaac ggcgaagagt cacccggaga cgacccggcc tacccgcttc
     1561 ccgtgctaca cgacgtcgac ggcatgccgc gcatcttcta catcggctgg tacatccgcg
     1621 acgcgcaacg ccacccagcc gtcccgcggc tcaccaacgc gcagcgcgcc gcgctcgagc
     1681 tgatcgagtc gatcgccaac gatcctgcct tccacgtcgt gatggacttc gagcgcggtg
     1741 atgtccagtt gctgaacaac gccaagatcc tccactcccg cgaggcctac gaggacgacg
     1801 aagacctcga caaccggcga cacttgctgc gactctggct cagcgctcgc cagttcacga
     1861 gcgtcgagga acgcctgcgc ggcggaatcc cgcaacgaca cagcaagggc cgctgacctg
     1921 caccgacgaa gactagggcg gaccgacccg gacgggcaga cgcctctcta cccgccctca
     1981 tgacccgaag ctcatcggtt caagtcccgc ccccgccacc attcgcaggt cgatcgctct
     2041 tcgtgcacga tgaccgaaga gtcggagcga agaattggca acccgcgggc gagggctctg
     2101 tcgctcttgc tggggccgtg tcaaacacca tgtcaaacgt gcctcgcgcg gcggccgcat
     2161 tgcatggcgg accggcccgc tgtgcgaggg gcgttcgagg agcttggcgg cctgcgtccg
     2221 gtatgcgcgg ctcacaacgg tgtcgctccg agcacggcct tcggtgacat gtcgtcaaca
     2281 accaagtgac tctcaggatc ggtgggctcc ctggtacgcg atcggagcga gtgggtagtc
     2341 atcgaggcat ggcgtcggga atctggccgc ttcgtcgcac tggtacgaaa ccttcctccg
     2401 acggggaagc cagccgatgc ccgaggtgat ggtgtggaag ctggaagctg gcggtgggct
     2461 gcaggtgtac gaggttcccg aacgagcagg tcgggatcct gcaccctgat cgtgagcgac
     2521 atcgacgaga tcgcgcgccg ggctcgacgg gaccggcctc gtcgcgagac ccgaggtcgc
     2581 ccacagcgat cgagtcgaca cgatcatggt caaggaccgg aatggcaacc cgatcgcatt
     2641 tgccaaccga aggatgcgtc gctccttcgc tgaattaccg gcgcatgcgt cgcgctcgaa
     2701 gcactcgggt tggcgtgtcg gggcggctcg tagcattcga gcgatgcctc gtacggaccg
     2761 cgccactcgt gtcatcgccg ccccgctgga ccgcgtcttc gacgcgctgg tcgatcacga
     2821 cgccctgctg gcatggcttc cgcctcgcgg catgacggcg aggttcgagc gattcgaccc
     2881 gaggccgggt ggctcgtacc ggctggtctt gacctacaca gatacagcgg actcccgtcc
     2941 aaagagctca cctgactccg acatcgtcga ggcgcggtac gtcgacatcg tcgccaacgt
     3001 tcgcgtcgtc caagcggtgg acttcatgtc tggagacccg cagttcgctg gcacgatgac
     3061 gatgacctgg gcggtggctg cagtcgatgg cggaacacgc gtcgacatca ctgctgacga
     3121 tgtgcccgac ggcatctcag ccgatgatca cgcgtcggga ctcacatcct cgctcgagaa
     3181 cctcgccacc tatatcgagt catagcgatc tcctccccca ggtcagggtc ggccctcacg
     3241 accaacaacc actgcaccgc aacagccgag gaagcagcca gcacccgcca gcaatctcac
     3301 ttgagtcgat agccgac
//
LOCUS       DATBFZ010000292         7207 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_3153053, whole genome shotgun sequence.
ACCESSION   DATBFZ010000292 DATBFZ010000000
VERSION     DATBFZ010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 7207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7207
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3153053"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            216..545
                     /locus_tag="VH419_08995"
     CDS             216..545
                     /locus_tag="VH419_08995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043789.1"
                     /translation="MNRPRNRALGIGAGVVLALGVSGVVALTFDGDTSAPTVSSSAST
                     TPNTDEVAASAAQVAWSSQPAHDRRQLCDAYNADSDVALAAIDGGIGGDDPVLLDAFD
                     SLLSETC"
     gene            986..1345
                     /locus_tag="VH419_09000"
     CDS             986..1345
                     /locus_tag="VH419_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020390763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY7043790.1"
                     /translation="MASTNTQSIDAPHETRPFRAHGHMDVVTLGDFTLGKGVFEPGWR
                     WSEDVKPIAGTDSCQFLHTGICIAGQMTIRMNDGTEINVGPGDVMLIEPGHDAWVVGD
                     DACVLYDTGVAAYAKPQ"
     gene            1376..1960
                     /locus_tag="VH419_09005"
     CDS             1376..1960
                     /locus_tag="VH419_09005"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011758060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEY7043791.1"
                     /translation="MVSALADVDWPVRTERLTIRRATLGDLEATWRFRRLDSVSEWLT
                     SAPATLEEYRTQFENPDRLANTLVFQLDGLVIGDLMVAVEDAWSQSEVADQAKGVQAE
                     LGWVVDPAHEGKGYTTEAVREVIRICFEELGMRRVTAGCFADNVGSRRLMERLGMRRE
                     THARRDALHRSGRWLDGMSYAMLADEWTGASDGR"
     gene            complement(1994..2425)
                     /locus_tag="VH419_09010"
     CDS             complement(1994..2425)
                     /locus_tag="VH419_09010"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY7043792.1"
                     /translation="MNPERVDGYELVAGPPSVDDYLALREQAGLSPATREQADAAVRG
                     AWAAVHVVEGASGRTVGMGRVIGDGGWYFHVIDMAVLPEHQRRGLGDAILDALLEHIR
                     GHAPPLAYVSLLADPPGRRLYARHGFTETAPDSVGMAQRLG"
     gene            2518..4200
                     /locus_tag="VH419_09015"
     CDS             2518..4200
                     /locus_tag="VH419_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012922850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY7043793.1"
                     /translation="MPGTDASTTRPAIVTIDDDPSVSRAVARDLRRQYGEVCRIVRGE
                     SGAETLDALRELKLRGEQVALILADYRMPQMNGIEFLEQAMDIFPFARRVLLTAYADT
                     DAAIQAINVVDVDHYLLKPWDPPEEKLYPVVSELLEEWKRAAHAPVPETKVIGHRWST
                     RSFQVRDFLARNRVPFRWYAATEPEAARLLTAADITDERCIPVVITPEGDVLRSPTDA
                     EIAARVGLTTDPAETFYDVIVIGGGPAGLGAAVYAASEGLRTVLVERTATGGQAGESS
                     RIENYLGFPDGISGSQLAERARRQAVKFGAEVVSAREVSSVESRGAARAVRFVDGGSV
                     EAHTAILATGVSYRQLEAPGAEALTGRGVFYGAALTEAMSCADQDVYVVGGANSAGQA
                     ALHFAQRSRTVTMLVRGSDLGTSMSSYLIAQIEATPNIRIRPSTTVVEAHGTDHLEGL
                     TLLDAASGEKESVDTQWLFIFIGAAPHTDWLGDTVLRDDNGFVIAGPDLLVDGDPPAG
                     WTLDRVPYHLETSVPGVFVAGDVRAESVKRVASAVGEGAMAVMLVHRYLAKL"
     gene            4217..5653
                     /locus_tag="VH419_09020"
     CDS             4217..5653
                     /locus_tag="VH419_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012922849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY7043794.1"
                     /translation="MTTIRNCTPDLLRTLFLFEALSEERLQYLCERGSVVQADPGWLY
                     REGEDAKCFYVLVDGSIVMYRKVHDEDVEVNRTSYRGSYVGAWTAYLGDLVPQEYDSS
                     VRVVEPSTFFELPASELRDVMQMWFPMAQHLLSGLFQGFTTRQRTTAQRERLLALGSL
                     SAGLTHELNNPAAAAVRATATLQQRVSKMREKLAALAAGHLDEAALQTLIKFQDDAAQ
                     RVATAPKLTALESADAEDELSDWLDAHGVARSLELAPTFVQAGIDSDWLDLVVNAAPT
                     ESLGGAIHWLYYTIDTELLMKEITDSTTRISTLLEAAKQYSQMDRSPHQMADLRELLD
                     STLIMLSGKIAPDITVVKDYDSSLPPVPAYAAELNQVWTNLIVNAVQAMGDKGRLTVR
                     TRLDDEYAVVEITDTGPGVPAEIQDRVFEPFFTTKPIGHGTGLGLDIAWRIVVNKHRG
                     YLKVTSAPGATTFTVRLPLEGPQIGETA"
     gene            5650..6000
                     /locus_tag="VH419_09025"
     CDS             5650..6000
                     /locus_tag="VH419_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020386890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="HEY7043795.1"
                     /translation="MTAGIQPEVLPSSVGCADCDAAHPQGWWVHLRRCAECGHVGCCD
                     TSPGQHASAHAAASGHRYIQSFEPGEDWFYDFETGETLTGPALAPPRARPDDQPVPGP
                     AGRVPSDWRRFIHV"
     gene            6074..7111
                     /locus_tag="VH419_09030"
     CDS             6074..7111
                     /locus_tag="VH419_09030"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEY7043796.1"
                     /translation="MSAGEHMAAEIAEQPDVWQRLLDGSPDRLIAVARRIQESRPRFV
                     LLTARGTSDHAALYAKYLVEIVLGLPAGLVSPSTTTVYGARPDLRDVLLLAISQSGGS
                     PDLVQTVEAARTAGALTVAVTNNPGSALDGAAELHLDVEAGVERAVAATKSYTAELLM
                     LYLLVATVAERERTAVRRLPDWGSRVLAEEPRVSALSKRLHPLERLMVTGRGYAYATA
                     REAALKLMETCYISAQAFSGADLMHGPLALVEAEIPILAVTSAGKGGVALRPTLQKLH
                     DAGGRVFCVGSFGLTEGALPDVPLPREVPEEIAPMLEILPFQQLSLSLALARGVDPDA
                     PRGLSKVTETL"
BASE COUNT         1220 a         2450 c         2427 g         1110 t
ORIGIN      
        1 tgttgcgcgc cgcccgggcg gcgatctgcg gcgttatcgt cggtcaacga tgctgcgcat
       61 cgcctccctc ctctgccttg cagctcatcc acccggacac cgctcacgac gagcaacgtt
      121 ggttgacgcg gcactagtac caaacgtcac cccgtcggcg gttccgccgt cgacggggtg
      181 ataccccacg aacgacgaaa cgtgaaagat caacgatgaa tagaccacgc aaccgagccc
      241 tcggcatcgg cgcgggcgtc gtcctcgccc taggggtgtc cggcgtcgtc gccctgacct
      301 tcgacggcga cacgtcagcg cccaccgtat cgagcagcgc cagcaccacc cccaacacgg
      361 acgaggtcgc cgcctccgca gcccaggtcg cgtggagcag ccagcccgcc cacgaccgca
      421 ggcagttgtg cgacgcgtac aacgccgaca gtgacgtggc gctggccgcg atcgacggcg
      481 gcatcggcgg cgacgacccg gtgctgctcg acgcgttcga cagcttgctc agcgagacgt
      541 gctgaccccc tgacaccgac gcggtccgtt ctgcgcgccg gctgctcggg tcctgacgag
      601 ggacgcagct gtcgggaaag cgaggacgcg acgtctggac cggcaacacc aagaccgcga
      661 acgcccacct cggtggcttc accaaggctg gcacgtggag cacaaccgac ctgactccgt
      721 acaccaacgc gccgctgacc ggctcggtga acgtggacgg acgactgaca catcgcatga
      781 cggcacaggc cgcgtccgag ccgccgggcg cgatttgcct ctccgacatc gcctcaccgc
      841 agctcgtcga tctcaccttt gacggcacgg cgggcgtggg cacgggaaag ccgtcatttc
      901 aaacgccgcc accgtgccac gctcctatgc tgtgggtgac gaggagtcgc gcccacggga
      961 ccagacgatc cgatgggagg tagtcgtggc atcaacgaac acgcaaagca ttgacgctcc
     1021 gcacgagacg cggccgttcc gcgcccacgg tcacatggac gtcgtgacgc tcggagactt
     1081 caccctcggc aagggtgtct tcgaaccggg ttggcggtgg tcggaggacg tcaagccgat
     1141 cgccggaacc gactcgtgcc agttcctgca caccggcatc tgcattgcgg gccagatgac
     1201 catccgcatg aacgacggaa cggagatcaa cgtcggcccc ggcgatgtga tgctgatcga
     1261 gcccgggcac gacgcctggg tggtcggcga cgacgcctgc gtgctctacg acaccggggt
     1321 cgccgcctac gccaagccgc agtagccgcg gtcgcggccc acgaaggctc gacgtatggt
     1381 ttccgcgctt gccgacgtgg actggcccgt gcgtaccgag cggctcacga ttcgacgcgc
     1441 cacgctcggc gacctcgagg cgacctggcg gttccgtcgg ctcgactcgg tgagcgagtg
     1501 gctgacgtcg gctcccgcaa cgctcgagga gtaccgcacc cagttcgaga atccggatcg
     1561 actcgccaac acactggtgt tccagctcga cggcctggtc atcggcgacc tcatggtcgc
     1621 ggtcgaggac gcctggtcac agtcggaggt ggccgaccag gccaaaggtg tccaggccga
     1681 gttgggatgg gtcgtcgacc ccgcccatga agggaagggc tatacaactg aggcggtacg
     1741 cgaagtgatc cggatctgct tcgaggagct cgggatgcgc cgcgtcaccg ccggttgctt
     1801 cgccgacaac gttggctcga ggcgactcat ggagcgtctc ggcatgcgcc gcgagaccca
     1861 tgcccgacgc gacgccctgc atcgctcggg acgctggttg gatggcatgt cgtacgccat
     1921 gctcgccgac gagtggacgg gcgcgtccga cggtcgatga tgcgacaacg gctgcgcgcg
     1981 cgcagaacgg tcctcacccg agtcgttgcg ccataccgac cgagtccggc gccgtctcgg
     2041 tgaagccgtg gcgcgcgtac agccgccgcc cggggggatc agcgagcagg ctgacgtagg
     2101 cgagtggcgg agcgtgacca cggatgtgct ccagcaacgc gtcgaggatc gcatcgccga
     2161 ggccgcggcg ttgatgctcc ggaagcacgg ccatgtcgat gacgtggaag taccagccgc
     2221 cgtctccgat gacgcgtccc atcccaacgg tgcggccgga tgctccctcc acgacatgga
     2281 cggctgccca cgcaccgcga acggccgcgt ctgcctgctc ccgcgtagcc ggtgacaacc
     2341 ctgcctgctc acgcagcgcc aggtagtcgt ccaccgacgg aggacctgcc acgagctcgt
     2401 acccgtcgac acgttctggg ttcacccggg catcatgtcc gagccagggc acgcgccacg
     2461 aagcacccgc cttagcatgc agaggacacg agagccatcg agcggggagg agcggcagtg
     2521 cccggtacag acgcgagcac gacgcgtccg gcgatcgtca cgatcgacga cgacccgagc
     2581 gtctcccgag ccgtggcgcg tgacctgcgc aggcagtacg gagaggtctg ccggatcgtg
     2641 cggggcgaga gcggggccga gacgctggac gcgctgcgcg agctcaagct gcggggcgag
     2701 caggtcgcac tgatcctggc ggactaccgg atgccacaga tgaacgggat cgagttcctc
     2761 gagcaggcga tggacatctt cccgttcgcg cgtcgcgtgc tgctgacggc gtacgccgac
     2821 accgacgccg ccatccaggc gatcaacgtc gtcgacgtcg accactacct gctcaagccg
     2881 tgggaccctc cggaggagaa gctgtacccg gtggtgagcg agctgctcga ggagtggaag
     2941 cgggcggccc acgcgcccgt gccggagacg aaggtcatcg gtcaccgctg gtcgacgcgc
     3001 tccttccagg ttcgcgactt cctggcccgc aaccgggtgc ccttccgctg gtacgccgcc
     3061 acggaacccg aggcggcccg gttgctgacc gccgcggaca tcaccgacga gcgatgcatc
     3121 ccggtggtca tcacaccgga aggcgacgtg ctccgctctc ccaccgacgc cgagatcgcg
     3181 gcccgcgtcg gcctcacgac agatccggcc gagaccttct acgacgtcat cgtgatcggc
     3241 ggtggcccgg ccggtctggg agctgctgtg tacgcagcgt ccgagggctt acggacggtg
     3301 ctggtcgagc ggaccgcgac cggtggtcaa gcaggggaga gctcacggat cgagaactac
     3361 ctcggcttcc cggacggcat ttccggttcc cagctcgccg aacgcgcacg gcgccaggcg
     3421 gtcaagttcg gtgccgaagt cgtcagcgcc cgtgaggtca gctccgtcga gtcccgcggc
     3481 gcggctcggg cagtccggtt cgtcgacggc gggagcgtgg aagcgcacac cgccatcctc
     3541 gcgacgggag tgtcctaccg gcagctcgag gcaccgggtg ccgaggccct gaccggcaga
     3601 ggagtcttct acggtgcggc cctcacggag gccatgagct gcgcggacca ggacgtgtac
     3661 gtcgtcggcg gcgccaactc tgccggccag gcggccctgc acttcgctca gcgcagtcgc
     3721 accgtgacga tgctcgtgcg agggtcggac ctgggcacgt cgatgtcgtc gtacctcatc
     3781 gcccagatcg aggcgacgcc caacatcagg atccggccga gcacgaccgt cgtcgaagca
     3841 catggcactg atcacctcga agggctgacc ctcttggatg ccgccagcgg tgagaaggaa
     3901 tcggtcgaca cccagtggtt gttcatcttc attggtgcgg cgccgcacac ggactggttg
     3961 ggcgacacgg tgctgagaga cgacaacggc ttcgtgatcg caggcccaga cctgctggtg
     4021 gatggcgacc caccggccgg ctggacgctg gaccgagtgc cttaccacct cgagaccagc
     4081 gttcccggtg tgttcgttgc cggcgacgtc cgggccgaat ccgtgaaacg ggttgcgtca
     4141 gcggtcgggg aaggggcgat ggcagtgatg ctggtccatc gctacctggc gaagctctga
     4201 accaggagag ggaaacatga cgaccattcg caactgcacg cccgacctgc tgcggacgct
     4261 gttcttgttc gaggcccttt ccgaggagcg gctgcagtat ctgtgtgagc gcggctcggt
     4321 cgtccaggcc gatcccggtt ggctgtaccg cgaaggtgag gacgcgaagt gcttctacgt
     4381 cctcgtcgac ggatcgatcg tgatgtaccg caaggtccac gacgaggacg tcgaggtcaa
     4441 ccggacctcg taccgcggct cgtacgtcgg agcctggacg gcgtacctcg gagacctggt
     4501 cccgcaggag tacgactcgt cggtccgggt ggtcgaaccg tccacgttct tcgagctgcc
     4561 cgcgtccgag ctgcgcgacg tcatgcagat gtggttcccg atggcgcagc acctgctgag
     4621 cggactcttc cagggcttca cgacccggca gcgcaccact gcacagcggg agcggctgct
     4681 ggcgctgggg tcgctgtctg ctgggctgac ccacgagctg aacaatcctg ccgcggccgc
     4741 tgttcgggcg acggcaacgc tccagcagcg cgtctcgaag atgcgcgaga aactggccgc
     4801 cctggccgcc ggtcacctcg acgaggcagc gttgcaaacg ctcatcaagt tccaggacga
     4861 cgccgcacag cgggtggcga ccgcgccgaa gctgaccgca ctggagtctg cggacgccga
     4921 agacgagctg tccgactggc tcgatgcgca tggcgtcgcg cgctcactcg agctggcgcc
     4981 gacgttcgtg caagccggca tcgacagcga ctggcttgac ctggtggtca atgccgcgcc
     5041 gacagagtcg ctgggcggcg cgatccactg gctgtactac accatcgaca ccgagctctt
     5101 gatgaaggag atcaccgact ccaccacacg catctccacc ctgctcgagg ctgcgaagca
     5161 gtactcccag atggaccgat cgcctcacca gatggctgat ctccgggagc tgctcgacag
     5221 cacgctcatc atgctcagcg gcaagatcgc tcccgacatc actgtcgtga aggactatga
     5281 ctcgtcgctg ccgccggttc cggcgtacgc cgccgagctc aaccaggtgt ggacgaacct
     5341 catcgtcaac gccgtgcagg cgatgggcga caaggggagg ctgaccgtcc gcacgcggct
     5401 cgacgacgag tacgccgtcg tggagatcac cgacaccggt ccgggagtcc cggcggagat
     5461 ccaggacagg gtcttcgagc cgttcttcac cacgaagccg atcggccacg gcaccggtct
     5521 cgggctcgac atcgcatggc gcatcgtggt gaacaagcac cgtggctacc tcaaggtgac
     5581 ctccgctccg ggagcgacga cgttcacggt gcgcctaccg ctcgaaggcc cacagatcgg
     5641 agaaacggcg tgaccgccgg gatccagccc gaggttctgc cgagcagtgt cggctgcgcg
     5701 gactgcgatg cagcacatcc gcagggatgg tgggtccatc ttcgtcgctg tgcggagtgc
     5761 ggccatgtcg gctgctgcga cacgtcaccg ggacagcacg cgtccgcgca tgcggcggca
     5821 tcagggcacc gctacatcca gagcttcgag ccgggcgagg actggttcta cgacttcgag
     5881 acgggcgaga cgttgacagg cccggcactt gcccctccga gggcccgccc cgatgaccaa
     5941 ccggtgccgg gtccggcggg tcgggtgccg tccgactggc ggcggttcat ccacgtgtga
     6001 catcgcaccc ggcatgtccg gcgcaagcct tttcgggtga taggtttccc ggcgttggct
     6061 ggaggtggag acgatgtcgg cgggcgagca catggcggcg gagatcgccg agcagcccga
     6121 cgtctggcag cgccttctcg atggatcgcc cgaccggttg atcgctgtgg cgcgacggat
     6181 ccaggagagc cggccgcggt tcgtgctcct caccgcccgc ggtacgtccg accacgccgc
     6241 gttgtacgcc aagtacctcg tcgagatcgt gctcggactt cccgccggac tcgtctcccc
     6301 gtctacgacc acggtgtacg gagcccggcc ggacctgcgc gacgtactgc tgctcgccat
     6361 cagccagtcc ggcggatcac cggacctcgt tcagacagtg gaggcggcac ggacagcggg
     6421 ggcgctgacc gtcgcggtca cgaacaatcc cggatccgcg ctcgatgggg ccgctgagct
     6481 gcacctcgac gtcgaagccg gcgtcgagcg tgcagttgcc gccaccaagt cgtacacggc
     6541 cgagctgctg atgctctacc tgctcgttgc caccgtcgcg gagcgagaac gcacagcggt
     6601 acgccggcta cccgattggg gcagtcgcgt gctggccgag gaaccgcggg tgtcggcact
     6661 cagcaagcgg ctccaccccc tcgagcgact gatggtgacc ggccgggggt acgcctacgc
     6721 gactgcccgg gaagcggccc tcaagctgat ggagacctgc tacatctcgg cgcaggcgtt
     6781 ctccggcgcc gacctgatgc acgggccgct ggcacttgtt gaggcggaga ttcccatcct
     6841 ggcggtcacg tctgctggca agggtggcgt cgcactccgg ccaaccctgc agaagctgca
     6901 cgacgccggc ggacgggtgt tctgcgtcgg gtcgttcggt ttgaccgagg gcgcgctacc
     6961 ggacgtgccg ctgccacgag aggtcccgga ggagatcgcg ccgatgctcg agatcctgcc
     7021 gttccaacag ctctcgttgt cgctggcgct cgcccgcggc gtcgaccccg atgcgccgcg
     7081 cggcctgagc aaggtcaccg aaaccctctg accgccggga cccgctgatc gagctccccg
     7141 ctgatcgagc tccccgctga tcgagcctgt cgagatcaag ggggtctcga caagctcgac
     7201 cgccgga
//
LOCUS       DATBFZ010000293         3351 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_199021, whole genome shotgun sequence.
ACCESSION   DATBFZ010000293 DATBFZ010000000
VERSION     DATBFZ010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 3351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3351
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199021"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="VH419_09035"
     CDS             <1..196
                     /locus_tag="VH419_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020387370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043797.1"
                     /translation="PAHVRYEPSWEAVPELVASVLAESAAPTLLVTMGAGDVTEIGPR
                     VLTLLRARAARGSPDGTAAV"
     gene            177..896
                     /locus_tag="VH419_09040"
     CDS             177..896
                     /locus_tag="VH419_09040"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HEY7043798.1"
                     /translation="MAPPPSESRFARRQWARRLRAASPWLLTALVVGLLVFAGWAVFF
                     SSWLALRQVDVAGTSMVSEREVVRAASVSPGTPLARVDLGDVRRQVAAIPAVADVTVH
                     RSWPHTLQITVSERTPSATVRRDGSWYAMDDAGVLFHPTKDRDPKLPVVVLAPSADAA
                     ARQEVASVVAALPDDIVSSMRRVRATSVDSITLTLRDGREVRWGSADESDRKVQVLAI
                     LLKQDASEYDVSVPELPTTRR"
     gene            complement(971..1705)
                     /locus_tag="VH419_09045"
     CDS             complement(971..1705)
                     /locus_tag="VH419_09045"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HEY7043799.1"
                     /translation="MRHLGILAHSTEGAALCFRVFCQEGFAALGPHDHPDVSMDCIAL
                     ARSMPAWNSGDHTFVRGVLGLSVDRLARAGADFFVCPDNTAHMALELPGEPLALPGLH
                     VCDIVATEAAERGYSTIGVLGTRYTMDGSLYPDALGRRELAYVLPEADDRELINAVIF
                     DELLNGELRLESRSAYVRIIADLADRGCDAVALVCTEIPLLVTPEVSPLPTLDSTRLL
                     ARAAFEVATHERPFPTWRGGPPSSSA"
     gene            2021..3208
                     /gene="ftsZ"
                     /locus_tag="VH419_09050"
     CDS             2021..3208
                     /gene="ftsZ"
                     /locus_tag="VH419_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEY7043800.1"
                     /translation="MAAPQNYLAVIKVVGIGGGGVNAVNRMIEVGLRGVEFIAINTDA
                     QALLMSDADVKLDIGRELTRGLGAGANPDVGGKAATDHAEEIEEVIKGADMVFVTAGE
                     GGGTGTGGAPVVARIARSLGALTIGVVTRPFSFEGRRRANQAEEGIAALREEVDTLIV
                     IPNDRLLSISDRNVSVLDAFKQADQVLLQGVSGITDLITTPGLINLDFADVKSVMQNA
                     GSALMGIGRANGDDRAVVAAEMAVSSPLLEASIDGAHGVLLSISGGSDLGLFEINEAA
                     ALVSQAAHAEANIIFGAVIDDALGDEVRVTVIAAGFDGGMPKRRDQSQPALRRDSAPA
                     PAAAQPPPEREVQPPAPQPEKVMVAAGVVSDGKPVASAVRQPRAIPFDDNDDLDVPDF
                     LKD"
BASE COUNT          560 a         1152 c         1131 g          508 t
ORIGIN      
        1 gccggcgcat gtgcggtacg agccgagctg ggaggcagtg ccggaactcg tggcatccgt
       61 gctcgcggag tctgcggcgc ccacgttgct cgtcacgatg ggtgccggcg acgtgaccga
      121 gatcggcccg cgcgtgttga cgctgcttcg ggcgcgggcc gcgagaggat caccagatgg
      181 caccgccgcc gtctgagtca cgcttcgccc gtcggcagtg ggctcggcgg ctgcgcgcgg
      241 ccagtccgtg gttgctcacg gcgttggtcg tgggcctgct ggtgttcgcc ggatgggccg
      301 tcttcttctc gtcctggctt gccctgcggc aggtcgacgt cgcaggaacc tccatggtgt
      361 ccgagcgtga ggtggtgagg gccgcctccg tttcccccgg tacgccgctg gcgcgtgtcg
      421 acctcggcga cgtacgccgg caggtcgctg ccatccctgc tgtcgccgac gtcaccgtgc
      481 accgaagctg gccgcacacg ctgcagatca cggtcagcga gcgaaccccg tcggcgaccg
      541 tgcggcgtga tggcagctgg tacgcgatgg acgatgccgg cgtgttgttc cacccgacga
      601 aggaccgcga tccgaagctg cccgtcgtcg tactcgcgcc gagcgccgac gcggccgcac
      661 gacaggaggt cgcctccgtc gtcgcggccc tgccggacga catcgtgtcg tcgatgcgac
      721 gggtacgggc cacgtcggtc gactcgatca cgctcacgct gcgcgacggt cgcgaggtgc
      781 gatgggggag tgccgacgag tccgaccgga aggtccaggt gctcgccatc ctgttgaagc
      841 aagacgcctc ggagtacgac gtgagcgtcc ccgagctccc caccacccgc cgctgacctg
      901 gtccccgcga attgcgggaa aggtcatgct ggatcggcag gtctgacctg atgtttcccg
      961 cattccgcgg tcatgcggag ctgctgggcg gaccgccgcg ccaggtgggg aacggacgtt
     1021 cgtgcgtggc gacctcgaag gcggccctgg ccagcaggcg ggtcgagtca agggtcggca
     1081 gcggagagac ctcaggcgtc acgagcagcg gaatctccgt acagaccaag gcgacggcgt
     1141 cacagccacg gtcggccagg tccgcgatga tgcggacgta cgccgatcgg gactcgagcc
     1201 gcagctcacc gttcaacagc tcgtcgaaga tcaccgcgtt gatcagctcg cggtcgtccg
     1261 cctccggcag cacgtaggca agctcgcggc ggccgagcgc gtcgggatac aacgagccgt
     1321 ccatcgtgta gcgagtgccc agcacgccga tcgtcgagta cccacgctcg gcggcctcgg
     1381 tcgccacgat gtcgcacacg tgcaggccgg gcagggccaa cggttcccca ggcagctcga
     1441 gtgccatgtg cgcggtgttg tccgggcaga cgaagaaatc ggcaccggct cgtgccagtc
     1501 ggtccacgct cagcccgagg acgccacgga cgaaggtgtg gtcgccgctg ttccatgccg
     1561 gcatcgaccg cgccagcgcg atgcagtcca tcgacacgtc gggatggtcg tgcggtccga
     1621 gcgcggcgaa gccctcctgg cagaacaccc gaaagcacag cgccgcacct tcggtgctgt
     1681 gcgccaggat gcccaggtgt ctcatcggac cgccgccttc gttgcacaat tctgtacccg
     1741 cgcgggcgtg tctccaccac cccgcggtcg ccaacgccta acgtcgtcag gcaacgcgag
     1801 gttgacataa ctataactct ctacttgagg gtaagggttc gaccaccacg gcttccccgg
     1861 tcccaccaac accagcacag cagcacacgc cagcacctac aactcagcac caccaactca
     1921 gcaccaccaa ctcagcacag gcagcagcgc agggcgagca cggacagggc tcgaaggcaa
     1981 gtcctcaccc gaggaagacc acaagcgaga ggcacgtgtc gtggcagcac cgcagaacta
     2041 cctggcggtc atcaaggttg tcggcatcgg cggcggaggc gtcaacgcgg tcaaccggat
     2101 gatcgaggtc ggactccgag gcgtggagtt catcgccatc aacaccgacg cgcaggcact
     2161 gctcatgagc gatgccgacg tcaagctcga catcggtcgc gagctcactc gcggactcgg
     2221 tgccggcgcc aaccccgacg tgggaggcaa ggccgcaacc gaccatgccg aggagatcga
     2281 agaggtcatc aagggcgccg acatggtgtt cgtcaccgcc ggcgagggcg gcggcaccgg
     2341 cacgggaggt gcgcccgtcg tcgccaggat cgcccggtcg ctcggggcgc tcaccatcgg
     2401 cgtcgtcacc cgaccgttct cgttcgaggg acgtcgccgg gccaaccagg ccgaggaagg
     2461 catcgccgcg cttcgcgaag aggtcgacac gctcatcgtg atccccaacg accggctgct
     2521 gtcgatctcc gaccgcaacg tgagcgtgct cgacgcgttc aagcaggccg accaggtgct
     2581 gctgcagggc gtctcgggca tcaccgacct catcaccact cccgggctca tcaacctcga
     2641 cttcgccgac gtcaagtccg tgatgcagaa cgccggctcg gccctcatgg gcatcggccg
     2701 cgccaacggc gacgacaggg ccgtcgtggc ggccgagatg gctgtctcga gcccgctgct
     2761 ggaggcgtcg atcgacgggg cgcacggcgt actgctctcg atctccggcg gctcggatct
     2821 gggcttgttc gagatcaacg aggcggccgc actcgtctcg caggcggcgc acgccgaggc
     2881 caacatcatc ttcggtgctg tcatcgacga cgcactgggc gacgaggttc gggtgactgt
     2941 catcgcggcc gggttcgacg gtggtatgcc gaagcggcgt gaccagtcgc agccagcgct
     3001 ccggcgcgac tcggcaccgg ctcccgcggc agcgcaaccg ccacctgagc gcgaggtgca
     3061 acctccggca ccccagcctg agaaggtgat ggtggcggcc ggcgtcgtct ccgacgggaa
     3121 accggttgcg tccgccgtcc ggcagcctcg ggccatcccg ttcgacgaca acgacgatct
     3181 ggatgttccc gacttcctga aggactagac gattgattcc aaggggagcc gcgaaccagg
     3241 caacttcctt ggaatcactc gtctcgacat ccgagccgac gctgggagga gtcaccggtg
     3301 ttcctgcgtc gcacccggct cgacacccct gtcggccgcg gcggcccggt c
//
LOCUS       DATBFZ010000294         3193 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_2493709, whole genome shotgun sequence.
ACCESSION   DATBFZ010000294 DATBFZ010000000
VERSION     DATBFZ010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 3193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3193
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2493709"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            146..1513
                     /locus_tag="VH419_09055"
     CDS             146..1513
                     /locus_tag="VH419_09055"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY7043801.1"
                     /translation="MSRRLQFHIFTMDSVSHIYHGMWRHPETKSLARHDLDTWVELAK
                     IAERGCMDSIFIADVAGLQGDYQGGWDIFPERGIHVPIGDPATLLPAMMHATENLGFV
                     YTSSMIQYPPFTFARKASTLDHLSKGRLGWNVVTSATENAYRNVGFDQILEHDERYEW
                     AEEYLEVVYKLWEASWEDEAVVADVKRGIYADPSKIHKINHKGRRFSVEGPHLLEPSP
                     QRTPVLFQAGASPAGRNFAARHAEGVFLMVDTPRDVPAVVRDIRDRVKAAGRPPEDVV
                     FLQGLSLVVGSTQEEAQRKAEELDEYIDIEASAAMRGGALGIDLATLDPQTPVTEVLE
                     RAPGLRGNLQLWIESEPNGDQLTVADLGRFFAQKSRIVGTPESIADQMQEWADAGVDG
                     FNFIQMVTPGTFADFVDHVAPELQRRGMMQREYSHGTLREKLFAGRGSHLPDHHTARS
                     HRHQH"
     gene            2292..2975
                     /locus_tag="VH419_09060"
     CDS             2292..2975
                     /locus_tag="VH419_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043802.1"
                     /translation="MNHGGRESVEVFTVDTTAGRPAATWIGCVVMPKGVWGNGVVALP
                     DGGFAVTKFFDPASRNTFADGGDVRVWRPSAGWSTVQGSELMAPNGIELSPDGKNLYV
                     AVYADRSIVSVPLQGGAVTRVADVPFLPDNLRWTEAGTLLVTGQDFTDGPSIIRCLRE
                     GTGCPPGFNIVEIDPVAKSATSIFQTDTREFGFATVAAPVGDEVWVGTLQHDRIARVG
                     GLPLKRKGG"
BASE COUNT          536 a         1036 c         1079 g          542 t
ORIGIN      
        1 gtatcctcaa ggttgactcc gctgcagccg cgttggagga tgtggactgg agcacgagac
       61 aagtccgtac gtggatgccg cgtcgacgca cggccctgac cagagcgtcg ggagtctgct
      121 cgttgaacat cagagagggt cacctatgtc gcgccgactg cagttccaca tcttcacgat
      181 ggattcggtg tcccacatct atcacggtat gtggcgacac ccggagacca agagcctggc
      241 tcgccatgac ctggacacct gggtcgagct ggcgaagatc gccgagcgcg gatgcatgga
      301 ctcgatcttc atcgccgacg tcgcgggcct gcagggcgac taccaaggcg gctgggacat
      361 cttccccgag cgcggaatcc acgtcccgat cggtgacccc gccacactcc tgcctgcgat
      421 gatgcacgcc accgagaacc tcgggttcgt ctacaccagc tccatgatcc agtacccgcc
      481 gttcaccttc gcgcggaaag cctccacgct cgaccacctc tcgaaggggc gactcggctg
      541 gaacgtcgtg accagcgcca ccgagaacgc ctaccgcaac gtgggcttcg accagatcct
      601 ggagcacgac gagcgctacg agtgggctga ggagtacctc gaggtcgtct acaagctgtg
      661 ggaagcgtcc tgggaggacg aggccgtcgt cgccgacgtc aagcgcggta tctacgccga
      721 ccccagcaag attcacaaga tcaaccacaa gggacgccgg ttcagcgtcg aggggccgca
      781 tctgctggag ccatcaccgc agcgaactcc tgtgctgttc caggccgggg cgtcaccggc
      841 gggccgcaac ttcgcggctc gtcacgctga gggcgtgttc ctcatggtcg acacgccacg
      901 tgacgtcccg gccgtggtgc gcgacattcg cgaccgggtg aaggcggcgg gcaggccacc
      961 tgaggacgtc gtgttcctgc aaggcctcag cctggtcgtc ggctcgaccc aggaagaggc
     1021 acagcgcaag gccgaggaac tcgacgagta catcgacatc gaggcctccg ccgcgatgcg
     1081 aggcggcgcg ctcgggatcg acctggcgac cctggacccg cagaccccgg tgaccgaggt
     1141 tctcgaacga gctccaggcc tccggggcaa cctgcagttg tggatcgaat cggagcccaa
     1201 cggcgatcag ctgacggtcg ccgacctggg tcggttcttc gcgcagaagt cgcgcatcgt
     1261 cgggaccccg gagtccattg cggaccagat gcaggagtgg gccgacgccg gggtcgacgg
     1321 cttcaacttc atccagatgg ttacgccggg cacgttcgcg gacttcgtcg accacgtcgc
     1381 cccggagctc cagcggcggg ggatgatgca gcgggagtac agccacggca ccttgcgcga
     1441 aaagctcttc gcggggcgag ggtcgcacct gcccgaccac cacacagccc gttcccaccg
     1501 acaccagcac tgacccggcg caccccgtga gctgagtgaa cagagccgga ggcgtgccgg
     1561 gggatcggcg gcacggcggg gttgggccgg ccgttcggga ggcgctgccg gtggctgact
     1621 gcgcatcgtg cttgtgctgt gcgccgtggt tggacgtgtg ctggtcgtcc gccgaatcat
     1681 cgaagtttca tacccgaaaa gcacggccct gtttcagacg catcaaggca gtcgcaattc
     1741 tgtggtgacg ggctctctcc ggtgagtggt actcgggcac gccgcaaatc tactcagaca
     1801 tctctatgga ggttgcagtg agctcaagtc ttcacgcagt tcgctcggcc cctactcggc
     1861 cggccggtgg cggatctcga ctggccaggt ctgtgcggac agggctcgcg gtcgcccttg
     1921 cggccgggct cgtcgcgtgt ggctcgcagg ggagtggcct cgacgcggac gcttctgcaa
     1981 aagccggtcc ggctaacggt caactgtcct tcctcgatgt tcccgccgcg ccagaggacc
     2041 tgctgccgct ccgcggtacg aagtgggtgg tggcgagtgc gatggcgcac ggagagggta
     2101 agccggggcg actcggggtg gtcgactccg agtcgggcga gtccgaagcg ctctggcccg
     2161 cgcctggagc tcgcatagag catgactccc gtgtctaccc cgaatgttct actccaccgg
     2221 acgacaaggt ggtgtcgccc cacggcgtga acctgtctgt gcaaagcccc ggccggttca
     2281 cgctttatgc cgtgaatcac ggtggacgcg agtcggtgga ggttttcacc gtcgacacga
     2341 ccgcaggccg tcccgccgcg acgtggatcg gctgtgtcgt gatgccgaag ggcgtatggg
     2401 ggaacggcgt cgttgccctg ccggatggtg gcttcgcggt cacgaagttc ttcgacccgg
     2461 cgtcgcggaa cacgttcgcc gacggcggcg acgtacgggt ctggagaccc agcgcgggct
     2521 ggtcgacggt gcagggcagc gagttgatgg ctcccaacgg gatcgaactt tccccggacg
     2581 gcaagaactt gtatgtggct gtgtacgcgg atcgctcgat cgtctcggtg ccgctccaag
     2641 gcggcgccgt cacccgcgtg gccgacgtgc cgttcctgcc cgacaacctg cgctggaccg
     2701 aggccggaac gttgctggta accgggcagg acttcaccga cgggccgtcg atcatcaggt
     2761 gccttcgaga gggaaccggg tgcccgcccg gtttcaacat cgtcgagatc gatcccgtcg
     2821 ccaagtcggc tacctcgatc ttccagaccg acacgcgcga gttcggcttc gccaccgtcg
     2881 cggcaccggt cggtgacgaa gtgtgggtcg gcaccctgca gcacgaccgc atcgctcgtg
     2941 tcggtggcct gcccctgaag cggaagggtg gttgaccatc ggcgtcggcg gtgcggtcat
     3001 gacccgtcga cgcgagcgag cgatcgtcca gggcggccct gcttggggcg gacccgcact
     3061 acgtgctgcc gatggaggtc ccgtcatgtc gattagcacg gccgaccgcg tcgagctgca
     3121 tgagctcgcc gcccggtact gcgacgtcat cgacgccggc gactggccga gcctcagatt
     3181 catcttcatg agc
//
LOCUS       DATBFZ010000295         4290 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_1513032, whole genome shotgun sequence.
ACCESSION   DATBFZ010000295 DATBFZ010000000
VERSION     DATBFZ010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 4290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4290
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1513032"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            341..970
                     /locus_tag="VH419_09065"
     CDS             341..970
                     /locus_tag="VH419_09065"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEY7043803.1"
                     /translation="MRRPLTIMSLSTAALIIGGTAAYADTIIGTPRDDRLVGTPRSDV
                     IHGRGGDDRIIGRGGSDDLDGGPGNDRAIGGPGRDGLVGKTGRDRLSGSGGNDDMTGS
                     AGNDVLNGGPGADTIVDWLATEFAPGGSTGPANADTINGGGGGDHIGFTDGRDTVRAG
                     GGGDRLRTENDRFADHIDCGPGFDVLIYFGTRDNQDTVLRCERVRTRAL"
     gene            complement(1007..1219)
                     /locus_tag="VH419_09070"
     CDS             complement(1007..1219)
                     /locus_tag="VH419_09070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043804.1"
                     /translation="MSQPLPDDDDFTWTDHAESSGDEDRSPSRDARRVVVVLLLAGVL
                     LVVLVAVLAHILFGIFGDMSNDVFVF"
     gene            1384..1686
                     /locus_tag="VH419_09075"
     CDS             1384..1686
                     /locus_tag="VH419_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017935321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEY7043805.1"
                     /translation="MALTVGLLATLTAKPGKESDLADFLVQGQQIAEAEAGTRVWYAF
                     RIDESTFGIFDAFDDEDGRQAHLTGQIPEALGQVGPDLLAADPDIKPVDVLATKPR"
     gene            complement(1719..2657)
                     /locus_tag="VH419_09080"
     CDS             complement(1719..2657)
                     /locus_tag="VH419_09080"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEY7043806.1"
                     /translation="MSEESTGTVLLAGAANLAIAIAKTVGGLLSGSTAMLAEAAHSVA
                     DTLNQVFLMAALRRSKRPPDVQHPFGYGMERYFWSLLAAVGIFVLGAGFSIYEGVRAI
                     LQPEELTDLMIVYTVLGLSFVFEGISWLRAVRQLRGEAEDRGRGTVEHLKATTDPTAK
                     TVAFEDTAALIGILLAALGVTLHATTGAGFWDGAASIAIGVLLIGVAYALGRQNKAFL
                     IGQSVSEELRNGIRDEIANAPGIDEVVEVLTMRLSPDEVLVAARVDIDDSATGGDLEG
                     YADEVDGRLRRRFPEVRHIFLDPTDIRDDTSAPASD"
     gene            complement(2664..3158)
                     /locus_tag="VH419_09085"
     CDS             complement(2664..3158)
                     /locus_tag="VH419_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020387772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5709 domain-containing protein"
                     /protein_id="HEY7043807.1"
                     /translation="MTEFPAETGDPIEDAALADVPDQEEQLSQEQPADSLVDRGVDDV
                     LDEGYSPPERYSSAERFGNTAQEMREGESFEERLRQEVPDEQVTYGDGPDSDPTRDPL
                     EIPPEDDYIGDGEVGEQRAGRLVDPNEGIGPDEESELVGEDVGIDGAAAGAEEAAVHI
                     IDEE"
     gene            complement(3499..4200)
                     /locus_tag="VH419_09090"
     CDS             complement(3499..4200)
                     /locus_tag="VH419_09090"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013161577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEY7043808.1"
                     /translation="MNPTPLRELVATDWAQALAPVEPTVAGLGDFLRAESAAGRRFLP
                     AGENILRAFGQPLADVRVLIVGQDPYPTPGHAVGLSFSVDARVRPLPPSLVNIYRELH
                     DDLGVETPQHGDLSTWADHGVLLLNRVLTVRAGAPGSHRGRGWEQVTDLAISSLVARG
                     GPLVAILWGRDAQSLRPALGDVPVIMSPHPSPLSASRGFFGSRPFSRANAALVAQGGA
                     PVDWSLPPDEPAAPD"
BASE COUNT          717 a         1489 c         1464 g          620 t
ORIGIN      
        1 tgcacccggc agcaccacca ggattcgcgc cgccacagcg gacggtcagg tgaccttcga
       61 cggtgggctc atcgccaacg gtccggccaa ccatccgact cgaccggcgc cgttcattcg
      121 cgtcgaccgg gagcggtgac ccaacaacgc ccgtgactgc ttgcgacgat gccatcacgc
      181 tccggcgtga cgctgaccga ttcgggccac gcgggacgga atcgccgcag ctggttacca
      241 cgatggcgat tgggtacctg ttccgctgac cccgagcacg gggagcgcgt gacgagacgc
      301 tcgcgcgcac gacctgttgt cgacagagga aacgataccc atgagacgcc ctctcaccat
      361 catgtccttg tccacggccg ccttgatcat cggaggtacg gccgcgtacg ccgacacgat
      421 catcggcacg ccgcgcgacg atcgcctcgt cggtacaccg cgaagcgatg tcatccacgg
      481 ccgcggcgga gacgaccgca tcatcggcag gggcggttcc gacgatctgg acggcggccc
      541 cggaaacgac cgcgccatcg gcggacccgg gcgggatgga ctggtcggga agaccggccg
      601 cgaccgcttg tcggggagcg gtggcaatga cgacatgacc ggcagcgccg gaaacgacgt
      661 gctcaacggt ggaccagggg ctgacacgat cgtcgactgg ctggcgacgg agttcgctcc
      721 cggcggcagc acgggtccag ccaacgccga cacgatcaac ggcggcggcg gaggcgatca
      781 catcggcttc accgacggcc gcgacaccgt gcgcgccggc ggcggtggcg accggctgcg
      841 gacggagaac gacaggttcg ccgaccacat cgactgcggt cccggtttcg acgtgctgat
      901 ctacttcggc acccgcgaca accaggacac cgtgctgcgg tgcgagcggg tcaggacccg
      961 ggcactctga gcgagcccag gtgcgggcga cgtggctcga atgacgtcag aagacgaaca
     1021 cgtcgttgga catgtcgccg aagatcccga acaggatgtg ggcgagtacg gcgaccagga
     1081 ccacgagcag cacgccggcc agcaagagca cgacgacaac ccggcgtgca tcgcgtgatg
     1141 gcgatcggtc ttcatcccca gagctctcgg cgtggtcggt ccaggtgaag tcgtcgtcgt
     1201 cgggcagcgg ctgactcatg aggtgagcat cgtaggcgcc ttcggcgccg cccaccacga
     1261 acttgacagg gcgacctgcc ggttccggcg tggtagcagc gaccgcgcag caatacggtg
     1321 ggcagcggtg cgccatcgac cggccgaccg ggccggtagg acgcgccggt gaaggagacc
     1381 gacatggcac tgacagtggg gctgttggca acgctgaccg ccaagccagg caaggagtcc
     1441 gatctcgccg acttcctggt ccagggtcag cagatcgccg aggcggaggc gggcacgcgc
     1501 gtctggtatg cgttccgcat cgacgagtcg accttcggca tcttcgacgc tttcgatgac
     1561 gaggacggcc gccaggccca cctgaccggc cagatcccgg aggcgctcgg ccaggtcggg
     1621 ccggacctgc tcgccgcgga cccggacatc aagccggtcg acgtgctcgc gacgaagcct
     1681 cgctgacccg ggcggctgag tcgcccgcta accccgggtc agtccgaggc cggcgcgctg
     1741 gtgtcgtcgc ggatgtcggt cgggtccaag aagatgtgtc ggacctcggg gaaccgcctc
     1801 ctgagccgcc cgtccacctc gtcggcgtac ccctccaggt cgccgccagt cgcgctgtcg
     1861 tcgatgtcca cccgcgccgc aaccagcacc tcgtcaggcg acagtcgcat cgtgagcacc
     1921 tcgacgacct cgtcgatccc gggggcgttg gcgatctcgt cgcgaatccc gttgcgcagc
     1981 tcctcgctga cggactggcc gatgaggaag gccttgttct gccgaccgag cgcgtacgcc
     2041 acgccgatca gcagtacgcc gatcgcgatc gacgctgcac cgtcccagaa gccggcgcca
     2101 gtggtggcgt gcagggtgac gccgagggcg gccagcagaa ttccgatcag cgctgcggtg
     2161 tcctcgaaag ccacggtctt cgcggtcggg tcagtggtgg ccttcaggtg ctccacggtg
     2221 ccgcggccac ggtcctcggc ctctccacgg agctgacgga ctgcccgcaa ccacgagatc
     2281 ccctcgaaga cgaaggacag gccgagcacg gtgtagacga tcatcaaatc cgtgagctcc
     2341 tcgggctgga ggatcgcgcg aacgccctcg tagatcgaga agccggcgcc cagcacgaaa
     2401 atcccgacgg cggcgagcag ggaccagaag tagcgctcca tgccgtaccc gaacgggtgc
     2461 tggacgtcag gcggccgctt gctgcggcgc agcgcagcca tcaggaagac ctggttgagc
     2521 gtgtccgcca ccgagtgcgc ggcctcggcg agcatcgcgg tggagccgga gagcagaccg
     2581 ccgaccgtct tggcgatcgc gatcgccagg ttggcggcac ccgcgagcag gaccgtgccg
     2641 gtggactcct cgctcaaccg acgtcactcc tcgtcgatga tgtggacggc ggcctcctcc
     2701 gctccggccg ccgcaccgtc gatgcccacg tcctccccga cgagctctga ctcctcgtcc
     2761 gggccgatgc cttcgttcgg gtcgaccagc cggcctgccc gctgctcacc gacctcaccg
     2821 tcgccgatgt agtcgtcctc gggcgggatc tcgagcggat cacgggtcgg atcggagtcc
     2881 ggtccgtcgc cgtacgtcac ctgctcgtcc ggcacctcct ggcgcagccg ctcctcgaat
     2941 gactcgccct cgcgcatctc ctgcgcggtg ttgccgaacc gctctgccga cgagtaccgc
     3001 tcgggtggcg agtacccctc gtccagcacg tcgtcgacgc cacggtccac caggctgtcg
     3061 gcaggctgtt cctgcgagag ctgctcctcc tgatcgggta cgtcggcaag cgccgcgtcc
     3121 tcgatcgggt cgccggtctc ggccgggaac tcggtcatct gtccaccttc ccacgcggta
     3181 ggacggtccg cccgcgcgat ggttcgacgg acgccggcgg catacgaccg ggaaggcccg
     3241 acgccgggcg tcgagacccg tggtcctgac ggcccgacga tgaggcctcc ggggcactgg
     3301 cgatcaggcg cgctgccccc agccccggag gtgagcagga gatacccact gcgtcctgct
     3361 tcatgcgcca ttgcacgaac gaacatactt cgcccgacat ctccggggtc tcgacaggct
     3421 cgaccgccgg atcgctcgaa cccgctgaac gagctcgtcg agtgatcccc gctgatcgag
     3481 gttgtcgagt tcgagggctc aatccggtgc cgcgggctca tcgggtggaa gggaccagtc
     3541 gaccggagcc ccgccttgcg ccaccagtgc cgcgttggca cggctgaacg gacgcgaccc
     3601 gaagaaacca cggcttgccg acaacgggga cggatgcggg ctcatgatca ccgggacgtc
     3661 accgagcgcc gggcgcagcg actgcgcgtc gcgtccccag aggatcgcca ccagcggtcc
     3721 gccgcgcgcc acgagagagg agatcgccag gtcggtcacc tgttcccagc cgcggccgcg
     3781 atgagacccc ggcgctccgg ctcggacggt gagcacccgg ttgagcagca gtacaccgtg
     3841 gtcggcccat gtcgacaggt cgccgtgctg cggtgtctcg acgccgaggt cgtcgtgcaa
     3901 ctcgcgatag atgttcacca ggctcggcgg caacgggcga acccgcgcgt cgaccgagaa
     3961 cgacagcccg acggcatgac cgggtgtggg gtacgggtcc tgtccgacga tgagcacgcg
     4021 gacgtcggca agcggctggc cgaaggcgcg caagatgttc tcgccggccg gaaggaagcg
     4081 ccgcccggcc gcgctctcgg ctcgtaagaa gtcgcccagg ccggcgaccg tcggctcgac
     4141 cggagccagc gcctgcgccc agtcggtggc gacgagctcg cggagcgggg tcggattcac
     4201 agcacagact ttgccgcaaa cttctccaca gatcgtgcct tggatgtgga caaacctggg
     4261 ggtccaacgg accggttcgg cgtgtcgatg
//
LOCUS       DATBFZ010000296         1976 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_533493, whole genome shotgun sequence.
ACCESSION   DATBFZ010000296 DATBFZ010000000
VERSION     DATBFZ010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1976
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_533493"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="VH419_09095"
     CDS             complement(<1..313)
                     /locus_tag="VH419_09095"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY7043809.1"
                     /translation="MAPRIRPADADDAEDIAQIWYDAWSDGHRGNVPEALLAYRTRDQ
                     FSPRAAERVPHSWVAEVDGRTVGFVTVDGDEVEQIFVDATARGTGLATLLLDEGVRTI
                     KA"
     gene            371..>1976
                     /locus_tag="VH419_09100"
     CDS             371..>1976
                     /locus_tag="VH419_09100"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin monoamine oxidase family protein"
                     /protein_id="HEY7043810.1"
                     /translation="MTQAGDGPADSSAEPAAGSSEDAGGGSAGGPGFWTRRRFLSALG
                     VAGGAGAVVGGMEVLGLFPDAEEHKQAFTPPRESDFHLQGRVNGVSVLILGAGDAGLA
                     TAYELEKAGYRTEILEARSRPGGRAWTVRGGTNDTDSEDNEQHATFRDGHYMNAGPAR
                     IPQGHTTLDYCRELRVPVEVFVNANPEAYVYRTPAPDARGPLTRRPVRRRAAKADLTG
                     YVNELLAKAIDQNALDTKLGPADQEALISYLQSTGALLPGSNRYAGSANRGYAKVPTA
                     GNDGGSVGRPYDLGDLLASGLGRTFGFEAEWDQAMPMFQPVGGMDQLPRAIAAQLRGT
                     IRYDAPVSKITLTSDGVTVEYSDASGTSHSTSAAYCVCTIPPTVLVDVDANWPSEVTS
                     ALGEVMTLPVGKLGLEYGRRFWEDDDRILGGITDTNLDISTIWYPSYGFHSETGGVLI
                     GYYTFADGSMQYAAMTPEDRARRAVEQGVRIHGEAYAKDLLSSFSVHWDLQPFSAGGW
                     ALWADRAGSTAYQTLLRPAERLYFAGD"
BASE COUNT          315 a          674 c          697 g          290 t
ORIGIN      
        1 cagccttgat cgtccggacg ccttcgtcga gcagcagggt cgcaagtccg gtccctcgcg
       61 ctgttgcgtc gacgaagatc tgctcgacct cgtcaccgtc gacggtgacg aacccgacgg
      121 tgcggccgtc gacctcggcg acccatgaat gcggcacccg ctcggcggca cgtggcgaga
      181 actggtcgcg ggtccgatac gccagcagcg cttccggcac gttgccgcgg tgcccgtcac
      241 tccaggcgtc gtaccagatc tgcgcgatgt cctcggcgtc gtcagcgtcg gcgggcctga
      301 tcctgggtgc catgaccacg acactagaac gacgttgtcg tcgtcgaccg gctgcgagat
      361 agtgagccgc atgacgcagg cgggggatgg gccggcggac tcctcggcgg aacccgcggc
      421 gggctcctcg gaggatgccg ggggcgggtc agccggaggg cccgggttct ggacccggcg
      481 acgcttcctc tccgcgctgg gtgtggccgg cggagcaggc gccgtcgtcg gcggcatgga
      541 ggtgctcggg ctgtttccgg acgccgagga gcacaagcag gcgttcaccc cgccgcgcga
      601 gtccgacttc cacctccagg gccgcgtcaa cggggtgtct gtgctgatcc tcggcgccgg
      661 cgacgcggga cttgccacgg cgtacgagct ggagaaggcg ggataccgca ccgagatcct
      721 cgaggcgcgc agtcgacctg gtgggcgcgc ctggacggtg cgcggtggca cgaacgacac
      781 cgacagcgag gacaacgagc agcacgcgac gttccgcgac ggtcactaca tgaacgccgg
      841 accggcacgc atcccgcagg gccacacgac gctcgactac tgccgtgagc tccgggtgcc
      901 tgtcgaggtg ttcgtcaacg ccaacccgga ggcgtacgtc tatcgcacgc ccgcgcccga
      961 tgcgcgcggt ccactgacgc gccgacccgt acgccgacgg gctgcgaagg ctgacctcac
     1021 cggctacgtc aacgagctgc tcgccaaggc catcgaccag aacgcgctgg acaccaagct
     1081 cggacccgcc gaccaggaag cgctgatctc gtacctgcag agcaccggtg cgctgctgcc
     1141 cggcagcaac cggtatgccg ggtcggccaa ccgcgggtac gccaaggtcc cgaccgcagg
     1201 caacgacggc ggatcggtcg gccggccgta cgacctgggc gacctcctcg cgtccggtct
     1261 gggcaggaca ttcggcttcg aggcggagtg ggaccaggcg atgccgatgt tccagccagt
     1321 cggcggcatg gaccagctgc cacgcgcgat cgcggcacag ctgcgcggga ccatccggta
     1381 cgacgcaccg gtctcgaaga tcaccctgac gtcggatggc gtgaccgtgg agtacagcga
     1441 cgcgtccggc acctcccaca gcacgagcgc ggcgtactgc gtgtgcacga tcccgcccac
     1501 ggtgctggtc gacgtcgatg ccaactggcc gagcgaggtc acgtcggcgt tgggtgaggt
     1561 gatgacgctg ccggtcggca agctcggtct cgagtacggc cggcgcttct gggaggacga
     1621 cgaccggatc ctcggcggca tcaccgacac caacctcgac atctcgacga tctggtatcc
     1681 gtcgtacggc ttccactccg agaccggtgg tgtgctgatc gggtactaca cgttcgccga
     1741 cggatcgatg cagtacgccg cgatgacacc cgaggaccgg gctcggcggg cggtcgaaca
     1801 gggcgtgcgc atccatggcg aggcctatgc gaaggacctg ctgtcgtcgt tctcggtgca
     1861 ctgggaccta cagccgttca gtgcaggcgg gtgggcgctg tgggcagacc gggccggctc
     1921 gacggcgtac cagacactgc tccgtccggc cgagcggctg tacttcgccg gcgacc
//
LOCUS       DATBFZ010000297         8021 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_1514108, whole genome shotgun sequence.
ACCESSION   DATBFZ010000297 DATBFZ010000000
VERSION     DATBFZ010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 8021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8021
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1514108"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..770
                     /locus_tag="VH419_09105"
     CDS             <1..770
                     /locus_tag="VH419_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020579103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY7043811.1"
                     /translation="CDDEIVESLRFHLRDLSVSFRFGEEVEKVEIGAEGTITTLASGK
                     RIPAETVMYSAGRQGATELLNLAAAGVEADGRGRIPVDEHFRTKVDHIYAVGDVVGFP
                     ALAATSMEQGRLAALHAMGEPATGMGDIQPIGIYTIPEISYVGRTESSLTADSVPYEI
                     GLSRYRELARGQIVGDPYGMLKLIVHARQRSLLGVHVFGTGATDLVHIGQAVMGCGGT
                     VDYLVDTVFNYPTLSEAYKVAALDATNKLRSLDRLLG"
     gene            complement(827..2851)
                     /locus_tag="VH419_09110"
     CDS             complement(827..2851)
                     /locus_tag="VH419_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008362156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein meaA"
                     /protein_id="HEY7043812.1"
                     /translation="MPEPQGASEGDQGRPLRDTPWVMRTYAGHSSAAESNALYRRNLA
                     KGQTGLSVAFDLPTQTGYDPDSPTARGEVGKVGVPVPHLGEMRRLFDGIPLAEMNTSM
                     TINATAMWLLALYEVVAEEQGADPASLTGTTQNDIVKEYLSRGTYVFPPEHSLRLTTD
                     LIAYTVDTMPKWNPINICSYHLQEAGATPTQELAFSLCTAIAVLDAVRDSGQVARDRM
                     GEVVARISFFVNAGVRFVEEMCKMRAFTKMWDALTLERYGVEDERQRRFRYGVQVNSL
                     GLTEAQPENNVQRIVLEMLGVTLSKDARARAVQLPAWNEALGLPRPWDQQWSLRMQQV
                     LAFESDLLEYDDIFAGSHVIEAKVAELVDGAQDEIDRIQAMGGAVAAIESGYMKQALV
                     ASHAQRRARIESGKEVVVGVNAYVESEPSPLTTDVNTAIQSSDPQAEAAAVVSTRNWR
                     ASRDDVEVQEALARLQTDAKSGANLMPATVAAARAGVTTGEWAGVLREIFGEYRAPTG
                     ISGVIGSAGAGDELSRVRETVRQTGEELGGRLRILVAKPGLDGHSNGAEQIAVRARDV
                     GFEVVYQGIRLTTEEIVAAAVAEDVDLVGVSILSGSHMVLVPQIVDGIRAQGLADVPV
                     VVGGIIPAADAEALRQQGVAAVFTPKDFGLTEIMARFVDEIRLAHHLPPL"
     gene            complement(3047..3370)
                     /locus_tag="VH419_09115"
     CDS             complement(3047..3370)
                     /locus_tag="VH419_09115"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEY7043813.1"
                     /translation="MVVGSTVTSLVKIGSHLDVRNVGHVRTMLNSVIGSTTDPADVIV
                     DMGALEIIDAAGLGMLTAAHLRAERAGKRLVLRNCSNDIRRVFAVTRLNRILHLDRRP
                     LDLTA"
     gene            complement(3649..4404)
                     /locus_tag="VH419_09120"
     CDS             complement(3649..4404)
                     /locus_tag="VH419_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003926732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY7043814.1"
                     /translation="MLIHDIAAVVTGGASGLGGATAAALAAKGARVVGLDLAEPIAAA
                     ATVDGVTLVEADVTDEEQVRAALDRAAEAGPLRMVVNCAGIGTAGRILSKNGPHDLAL
                     FRKVVDVNLVGTFNVLRLGAEAMTQVDPDESGQRGVVVNTASIAAYDGQVGQVAYAAS
                     KGGIVGMTLSAARDLASFGIRVMTIAPGIVDTPMLAGVNEEFRATLAEGVPFPKRLAR
                     PEEYAALAVMIAEHDYLNGEVIRMDGALRMAPR"
     gene            complement(4398..4991)
                     /locus_tag="VH419_09125"
     CDS             complement(4398..4991)
                     /locus_tag="VH419_09125"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016456213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEY7043815.1"
                     /translation="MVNLTKIYTRTGDAGTTALGDMSRTSKTDPRLAAYADVDEANSH
                     IGYAISLGGLDDDVVTVLVHVQNDLFDVGADFCTPVVPDPKYPPLRVEQEYVDRLEAW
                     CDTYNEGLDKLRSFILPGGERGAALLHVARTVVRRAERSAWAAYATSADSMNVTAITY
                     LNRLSDLLFILARHANRERGDVLWRPGGERGQASPPC"
     gene            complement(5125..5697)
                     /locus_tag="VH419_09130"
     CDS             complement(5125..5697)
                     /locus_tag="VH419_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009072868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEY7043816.1"
                     /translation="MTARPDVSVCVFCGSSPGRGNGYRAAAAGFGTLLAERGIRLVYG
                     GASVGTMGVIADAALEAGGEVVGVIPRQLVDREIAHTGLTRLHVVGDMHERKARMAEL
                     ADGFVALPGGAGTLEEFFEVWTWGQLGLHAKALGLLDVDGFFAPLAAMLDHMVDEGFL
                     RPAYRDAVVLESDPGLLLDHVMPRWQHASL"
     gene            complement(5694..6023)
                     /locus_tag="VH419_09135"
     CDS             complement(5694..6023)
                     /locus_tag="VH419_09135"
                     /inference="COORDINATES: protein motif:HMM:NF019491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HEY7043817.1"
                     /translation="MSDLPALQHVVLMKFPTALSAAEDAKLRSMVASWPSEIGTMTEL
                     RFGGDLTGDRSRGYQYLLLTVFPDADTLAAYVAHPVHQELITFLNERDCERLAFDYYL
                     DDSTRPV"
     gene            complement(6016..6507)
                     /locus_tag="VH419_09140"
     CDS             complement(6016..6507)
                     /locus_tag="VH419_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2550 domain-containing protein"
                     /protein_id="HEY7043818.1"
                     /translation="MAWWEAILDSLGLVLVAILLVFVFLFVRRRVISRSGGTFECSVR
                     TRVPAQGAAASSARGWSLGLGRYTGDQLEWFRIFSFSARPKYTFDRTMTVGQRRTPHR
                     AEAFSLYAGHVVVAVQLDTGRRVELAMSHNALMGFLAWTEAAPPGFDPTTGRYKQRGE
                     RDV"
     gene            complement(6646..7029)
                     /locus_tag="VH419_09145"
     CDS             complement(6646..7029)
                     /locus_tag="VH419_09145"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020386452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEY7043819.1"
                     /translation="MADALQVELVSADRVVWSGEATMVVARTTDGEMGVLKDHAPVLS
                     VLVPSAVEIVTDDERLYAVIDGGFISVANNRLSVLAEHAQMANEVDLEQARRDLEEAE
                     ANAETPQDAALLLAQARVTVGEKAR"
     gene            complement(7029..>8021)
                     /gene="atpD"
                     /locus_tag="VH419_09150"
     CDS             complement(7029..>8021)
                     /gene="atpD"
                     /locus_tag="VH419_09150"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017935512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEY7043820.1"
                     /translation="LLTPYVQGGKIGLFGGAGVGKTVLIQEMIARVARDHGGVSVFAG
                     VGERTREGNDLIAEMTESEVIGQTALVFGQMDEPPGTRLRVALSALTMAEYFRDVQNQ
                     DVLLFIDNIFRFTQAGSEVSTLLGRMPSAVGYQPNLADEMGVLQERITSTRGHSITSM
                     QAIYVPADDYTDPAPATTFAHLDATTELSREIASLGIYPAVDPLTSTSRILDPRFISQ
                     DHYDTAVRVKQILQRNKELQDIIAILGVDELSEEDKIVVSRARRIQRFLSQNTYVARQ
                     FTGIEGSTVPLDETVEAFTKICDGEYDHVAEQAFFMCGGLEDVERKWAEIQKEL"
BASE COUNT         1274 a         2816 c         2635 g         1296 t
ORIGIN      
        1 tctgcgacga cgagatcgtg gagtcgctgc ggttccacct gcgcgacctg tcggtgtcgt
       61 tccgcttcgg cgaggaggtc gagaaggtcg agatcggcgc cgaggggacc atcaccacct
      121 tggccagcgg caagcggatt ccggccgaga ccgtgatgta ctccgccgga cggcagggag
      181 cgaccgagct cctcaacctc gcggcggcgg gtgtcgaggc agacggtcgc ggccggatac
      241 ctgtcgacga gcacttccgc accaaggtgg accacatcta tgccgtcggc gacgtcgtcg
      301 gcttcccggc actggcggcg accagcatgg aacagggccg gctcgccgcg ttgcacgcca
      361 tgggcgaacc tgccacgggc atgggcgaca tccagcccat cggcatctac acgatcccgg
      421 agatctccta cgtcggtcga acggagtcct ctctgacggc cgattccgtg ccgtacgaga
      481 tcggactgtc ccgctaccgc gagctggcac gagggcagat cgtcggcgat ccgtacggga
      541 tgctcaagct gatcgtccat gctcggcagc ggagtctgct cggcgtccac gtgttcggca
      601 ccggcgccac cgacctcgtc cacatcggcc aagcggtgat gggctgcgga ggaacggtcg
      661 actatctcgt cgacaccgtc ttcaactatc cgaccctgtc ggaggcctac aaggtcgccg
      721 cactcgacgc caccaacaag ctccgctcgc tggaccgtct cctcggctga ccccgacctc
      781 cgaccgctgc cggtccccgc gaattgcggg agcccaccgg ccgccgtcag aggggaggaa
      841 ggtgatgcgc gagtctgatc tcgtcgacga agcgcgccat gatctcggtg aggccgaagt
      901 ccttcggtgt aaagacggcg gccacgccct gctgccgcag cgcctcggcg tcggcggccg
      961 ggatgatgcc gcccaccacg acgggcacgt cggccaagcc ctgcgcccgg atgccgtcga
     1021 cgatctgcgg cacgagcacc atgtgtgacc cggacaggat cgacactccg acgaggtcca
     1081 cgtcctccgc caccgcagcg gcgacgatct cctccgtggt gagacggatg ccctggtaga
     1141 cgacctcgaa cccgacgtcc ctggcccgga ccgcgatctg ctccgccccg ttgctgtgcc
     1201 catccagtcc cggcttcgcc accaggatcc gcagccgccc gccgagctcc tcgccggtct
     1261 gccggaccgt ctcacggact ctcgacagct cgtctcccgc gccggcgcta ccgatcacgc
     1321 cggagatccc tgtgggagcc cggtactcgc cgaagatctc gcgcagcacg cccgcccatt
     1381 cgccggtggt gacccccgcc cgggcagcgg cgaccgtggc gggcatcagg ttggcgcccg
     1441 acttcgcgtc ggtctggagt cgtgcgagcg cctcctgaac ctcgacgtcg tcccggctcg
     1501 ctcgccagtt ccgggtcgac accaccgcag cggcctcggc ctgcgggtcg gatgactgga
     1561 tcgcagtgtt cacgtcggtc gtcaacgggg agggctcgga ctcgacgtac gcgttgacgc
     1621 cgaccacgac ctccttgccg gactcgatcc gtgcccggcg ctgcgcgtgc gaggccacca
     1681 gcgcctgctt catgtagccg gactcgatcg ctgcgaccgc gccacccatc gcctggatcc
     1741 ggtcgatctc gtcctgtgcg ccgtcgacga gctcggcaac cttcgcctcg atgacgtggg
     1801 agccggcgaa gatgtcgtcg tactccagca ggtcggactc gaacgccagc acctgctgca
     1861 tccggagaga ccactgctgg tcccacggcc gcggcagtcc cagcgcctcg ttccatgccg
     1921 gcagctggac cgcgcgggcg cgggcgtcct tcgacaacgt cacgccgagc atctccagga
     1981 cgatccgctg gacgttgttc tccggttgag cctcggtgag tccgagcgag ttgacctgta
     2041 cgccgtaccg gaagcggcgc tgccgctcgt cctcgacgcc gtaccgctcg agcgtgagcg
     2101 cgtcccacat cttcgtgaac gcccgcatct tgcacatctc ctcgacgaac cgcaccccgg
     2161 cattgacgaa gaacgagatc cgcgcgacga cctcacccat gcggtcccgc gccacctgtc
     2221 cggaatcccg gacggcgtcg agcacggcga tcgcggtgca gagcgagaac gccagctcct
     2281 gcgtcggcgt ggcccccgcc tcctgcaagt ggtagctgca gatgttgatc gggttccact
     2341 tcggcatggt gtcgacggtg taggcgatca ggtccgtcgt gagacgcagg gagtgctctg
     2401 gagggaacac gtacgtgccg cgggagagat actccttgac gatgtcgttc tgggtcgtcc
     2461 cggtgagcga tgccgggtcc gcaccctgct cttcggcgac gacctcgtac agcgccagca
     2521 gccacatcgc ggtggcattg atcgtcatcg aggtgttcat ctcggcgagc ggaatgccgt
     2581 cgaagagccg ccgcatctcg cccaggtgtg ggaccggcac ccctaccttg ccgacctctc
     2641 ccctcgccgt cggcgagtcc gggtcgtagc cggtctgcgt cggcaggtcg aacgccaccg
     2701 acaggcccgt ctgccctttc gcgaggttgc gtcggtacag cgcgttggac tcggcggcag
     2761 agctgtggcc ggcgtacgtc cgcatgaccc agggcgtgtc acgcaggggt cggccctggt
     2821 caccttccga ggcgccctgc ggttccggca tgctccgagg gtacggcggc ccgtcccccg
     2881 cgtggccccc gtgttacccg cctcacaccc ccacgacccc cggcgaggtg gcaggtttcg
     2941 ttcgtcctgg gcggcgtgtc gaaccgcgat tgccacctcg atgcacgcaa agcgtcggct
     3001 tgcggtgagc agggccgcaa gccgacggga atgtggttcg actgggtcag gcggtgaggt
     3061 cgaggggtcg ccggtcgagg tgaaggatcc ggttgagccg cgtcaccgcg aagacgcggc
     3121 ggatgtcgtt ggagcagttg cgcagcacga gccgcttgcc ggcgcgctcc gcgcgcagat
     3181 gagcggcggt cagcatgccg agtccggccg cgtcgatgat ctcgagggct cccatgtcga
     3241 cgatgacatc ggctgggtcc gtggtggacc cgatgacgga gttgagcatc gtgcgtacgt
     3301 gccccacatt ccgcacgtcg agatgagacc cgatcttgac caaactcgtg acggtcgaac
     3361 cgacgaccat gacacctccc cgctggtgct gccgcttgta ctcagtatga cgtccctggg
     3421 taccccaaag gttgtccctc gccgaagagc atgtccaaac attttggatg actgtttggt
     3481 aacgatgccc gttttccagg ggtctggacg tcttcgccaa gccttcagga gtacgacgct
     3541 acgcgccgaa tacgtcggac ctggatattt ccggtggcga gtctgcgacg ccgagatgac
     3601 gggaaggcta tgcgcccgtc attcggccgt gcgaagactt gccgcacctc agcgcggcgc
     3661 catccgcaac gcgccgtcca tcctgatcac ctcaccgttc aggtagtcgt gctcggcgat
     3721 catcacggcc aaggcggcgt actcctccgg ccgggcgagc cgcttcggga acggcactcc
     3781 ctcggccagt gtggcccgga actcctcgtt gacacctgcc agcatcggag tgtcgacgat
     3841 gcccggcgca atcgtcatca cccggatccc gaagctggcc aggtcgcggg cggcgctgag
     3901 cgtcatcccg acgatcccac ccttcgaggc ggcgtacgcc acctggccga cctgaccgtc
     3961 gtacgccgcg atcgaggccg tgttgacgac gacgccgcgc tggccgctct cgtccggatc
     4021 gacctgcgtc atcgcctcgg cgccaagtcg cagcacgttg aacgtgccca ccaggttgac
     4081 gtcgacgacc ttccggaaca gcgcgaggtc gtgtgggccg ttcttcgaga ggatgcgacc
     4141 agccgtgccg attccggcgc agttcaccac catgcgcagc ggccccgcct cggccgctcg
     4201 gtcgagggcc gcccggacct gctcctcgtc ggtgacgtcg gcctcgacga gcgtgacgcc
     4261 gtcgacggtg gcggcggccg cgatcggttc tgcgaggtcg agcccgacga cccgcgcgcc
     4321 cttggcagcc agcgctgcgg cggtggcgcc gcccaggccg gaggcgccac cggtgacgac
     4381 ggcggcgatg tcatggatca gcatggcggc gaggcctgcc cgcgctcgcc gcccggtcgc
     4441 cagaggacgt ctccacgctc ccggttggcg tgtctggcca ggatgaagag caggtcgctg
     4501 aggcggttga ggtaggtgat cgcggtgacg ttcatcgagt cggcggacgt cgcgtacgcg
     4561 gcccaggctg acctctcggc ccggcgtacg acggtgcgcg ccacgtgcag gagggcggcg
     4621 cctcgctcgc cgccgggcag gatgaaggac cgcagtttgt cgagtccctc gttgtacgtg
     4681 tcgcaccacg cttcgagccg gtcgacgtac tcctgctcca cccgcagcgg tgggtacttc
     4741 gggtccggca cgacgggggt gcagaagtcg gccccgacgt cgaacaggtc gttctggacg
     4801 tggaccaaca cggtgacgac gtcgtcgtcc agaccgccga gcgagatggc gtacccgatg
     4861 tgcgagttcg cctcgtccac gtcggcgtac gccgccaggc gcggatcagt cttcgacgtc
     4921 cggctcatgt cgcccaatgc ggtcgtcccg gcatcgccgg tgcgggtgta gatcttcgtc
     4981 aggttgacca tgcggcaagc ctatggccga gcgagtcctc ggccgaggtg gcgatcgacg
     5041 ttcgacacgc cgccgaactc caacaaaacc tgccacctcg ccggaactcc tggcgaggtg
     5101 gcaggtttgg ttgcagacac gccgtcagag ggacgcgtgt tgccacctcg gcatgacgtg
     5161 gtcgaggagc agaccgggat ccgactccag tacgacggcg tcgcggtacg ccggccgcag
     5221 gaaaccctcg tccaccatgt ggtccaacat cgcagcgagt ggcgcgaaga agccatccac
     5281 gtcgagcagt cccagcgcct tcgcgtgcaa cccgagctga ccccaggtcc acacctcgaa
     5341 gaactcctcg agcgtcccgg ccccacccgg cagagcgacg aacccgtccg cgagctcagc
     5401 catccgggcc ttgcgctcgt gcatgtcgcc gaccacgtgc agtcgcgtca gaccggtgtg
     5461 cgcgatctcg cgatcgacca gctgccgcgg gatcacaccg accacttcgc cgcccgcctc
     5521 gagtgcggcg tcggcgatga cgcccatcgt cccgacgctg gcgccgccgt agacgaggcg
     5581 gatgccacgt tcggcaagga gcgtgccgaa gcccgcggcg gctgcgcgat acccgttgcc
     5641 gcggccgggt gacgagccgc agaagacaca gacggagacg tccgggcgag ccgtcatacc
     5701 ggtcgtgtcg agtcgtcgag gtagtagtcg aaggcgagtc gttcgcagtc gcgctcgttc
     5761 aggaacgtga tcagttcctg atgcaccgga tgggcgacgt acgccgccag cgtgtcggcg
     5821 tccgggaaca cggtcagcag caggtactgg tacccgcgcg agcggtcgcc ggtgaggtcg
     5881 ccaccgaacc gcagctcggt catggtgccg atctcgctcg gccagcttgc gaccatggac
     5941 cgaagtttcg cgtcctcggc agcggacagg gccgtgggga acttcatcaa cactacgtgc
     6001 tggagcgctg gcaggtcaga catcgcgctc gccacgctgc ttgtaccgac ccgtcgtcgg
     6061 gtcgaacccg ggtggtgccg cctccgtcca tgccaggaaa cccatcagcg cgttgtggct
     6121 catcgcgagc tcgacccgcc gaccggtgtc gagctgcacc gcgacgacca cgtgaccggc
     6181 atacagcgag aacgcctcgg cacggtgtgg tgtgcgtcgc tggccgaccg tcatggtgcg
     6241 gtcgaaggtg tatttcggac gcgccgagaa cgagaagatc cggaaccact ccagctggtc
     6301 accggtgtat cggccgaggc cgagtgacca gccccgggcc gacgacgcag cagcgccctg
     6361 tgccggcaca cgtgtacgta cgctgcactc gaaggtgccc ccactgcggc tgatcacccg
     6421 gcgacgaacg aacaggaaga cgaacaccag caggatcgcg accaggacca gacccaggct
     6481 gtcgaggatc gcctcccacc aagccatcgt cacctctcac cggcgcctcg acgacgcgta
     6541 ccgatccccc cacgggcaag agcctaccga gcctgccgcc acgagtgacg acgcgcaggt
     6601 gtcggcgacg gacgaacctg cgctccgtca gaccggtgat gcggcctacc tggccttctc
     6661 ccccaccgtg acgcgagcct gggccagcag gagtgccgcg tcctgcggcg tctcggcgtt
     6721 cgcctccgcc tcctccaggt cgcggcgggc ctgctcgagg tcgacctcgt tcgccatctg
     6781 ggcgtgctcg gcgagcaccg acagccggtt gttcgcgacc gagatgaacc ccccgtcgat
     6841 cacggcgtag aggcgctcgt catcggtgac gatctccacc gccgacggca cgagcaccga
     6901 cagcacgggt gcgtggtcct tgagcacgcc catctcaccg tcggtggtcc gggcgacgac
     6961 catggtcgcc tccccggacc acaccacccg gtcagccgag acgagctcga cctggagcgc
     7021 gtcagccatc agagttcctt ctggatctca gcccacttgc gctcgacgtc ctcgaggcca
     7081 ccgcacatga agaacgcctg ctcggccacg tggtcgtact caccgtcgca gatcttggtg
     7141 aacgcctcga ccgtctcgtc gagggggacc gtcgaaccct cgatgcccgt gaactgcctg
     7201 gccacgtacg tgttctgcga caggaaccgc tggatgcggc gggctcgcga cacgacgatc
     7261 ttgtcctcct cggacagctc atcgacaccc aggatcgcaa tgatgtcctg cagctccttg
     7321 ttgcgttgca ggatctgctt cacccggacc gccgtgtcgt agtggtcctg gctgatgaag
     7381 cgcgggtcca ggatccggga ggtcgacgtc agcgggtcca ccgccgggta gatgccaagc
     7441 gacgcgatct cacgggacag ctcggtcgtg gcgtcgagat gcgcgaacgt cgtcgccggc
     7501 gccggatcgg tgtagtcgtc ggccggcacg tagatcgcct gcatcgacgt gatcgagtgg
     7561 ccgcgcgtcg aggtgatccg ctcctgcagc acgcccatct catcggccag gttcggctgg
     7621 tagcccaccg ccgacggcat ccggccgagc agcgtcgaca cctcggaacc ggcctgggtg
     7681 aaccggaaga tgttgtcgat gaagagcagc acgtcctggt tctgcacgtc gcggaagtac
     7741 tccgccatgg tcagcgcgga cagcgcaacc cgcagtcggg tgcccggcgg ctcgtccatc
     7801 tggccgaaca cgagcgcggt ctgtccgatc acctcggact cggtcatctc cgcgatcagg
     7861 tcgttgccct cgcgggtgcg ctcgccgacg ccggcgaaca ccgacacacc accgtggtcg
     7921 cgcgcgaccc gggcgatcat ctcctggatg agcacggtct tgccgacacc cgcgccgccg
     7981 aacaggccga tcttgcctcc ctgcacgtac ggcgtgagca g
//
LOCUS       DATBFZ010000298         2542 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_3485544, whole genome shotgun sequence.
ACCESSION   DATBFZ010000298 DATBFZ010000000
VERSION     DATBFZ010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3485544"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            320..532
                     /locus_tag="VH419_09155"
     CDS             320..532
                     /locus_tag="VH419_09155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043821.1"
                     /translation="MTTSTMAVHGDIVAALRNELLELAAQQDALADSEAAKVPYWAPC
                     PPHVEGHRVAALALRADADRLIAVSA"
     gene            643..>2542
                     /locus_tag="VH419_09160"
     CDS             643..>2542
                     /locus_tag="VH419_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043822.1"
                     /translation="MRRRLPIVFVVVGSLLLAAGLVAGIINREVIDGSRFVDHVDAIR
                     QDPDVSRQVGLALSDRVIEADPDLVVVRPLLESAAQALVAEPAFGIVARASAAQFHNA
                     FTESGPVVIRVADVGAVLTAVLSAYAPQASAKLPANLDATLARVGSQTFAADTIHLTE
                     KVRLLAWLLPLLALLAFAVAVVVTSNRRRAMAAVGWAVFAAGALLACLGFGLWLVTSF
                     ADTDTLSGALVVAGWRELQQPVWLTAAGAMVAGAVFGVAVARSGRRSVPEILAAGWAW
                     MTRDSGRHHERLLRAVVWLGIGVGLVVRPLVFVAAIAVALGVAAVVTGANGLLAVAKE
                     RVSVPRRRPRFAVDPRWWRAVGITLGVALLVSLLVALFQPVDETIAAGSQSAYSGPCN
                     GHVDLCVRRYDDVAYAATHNSMAAADEPNWFLAEQPTGLVGQLDDGIRVLLIDTWYGQ
                     ATQRRDIVATASRSRQRATAEAVDRYGQSVVDAALRLRDAASLTPSGPVEPYLCHSMC
                     ELGSTKFVPALVQMRAWLDAHPREVVTLFIQDEVTPADTAEAFEQAGLLSYVHTQQTG
                     GSWPTLGEMIASGRRLVVLMENHGGGSTYPWLLQGFDWVQDTPYDYTSPSQFSCRRYR
                     GSAASPLFL"
BASE COUNT          355 a          843 c          879 g          465 t
ORIGIN      
        1 gttggactcg aactgcttgg tgaaccgttc gaggtcgtcg ctgttcaacg cgcggctgat
       61 gtcgtctgcc cttgctgcca tgtccgtcct tcggggtcag ggtgcgagcg gtgacgtgac
      121 gctccgcccc gagcctacgc tgcgactgtc cagtaccacc agaacgctga ctccgacccc
      181 gtcccgggca ggacaggtgt cctgcggcgc gggacgtcca ccgaaaaatc gccgggctcg
      241 atgaatcttt cagcgatttc cagtctcctc aggtaccagg accgaatccc aagtgtggcg
      301 acgaggaagc gaggcgacca tgaccacatc cacgatggcg gtgcacggcg acatcgtcgc
      361 tgcgctgcgg aacgagctgc tcgagctggc tgcccagcag gacgcgctcg ccgactcgga
      421 ggccgcaaag gtcccgtact gggcgccgtg ccctccgcat gtcgaaggcc accgggtcgc
      481 cgcgctggcg ctgcgcgccg acgccgatcg actgatcgct gtctcagcct gacgaacagg
      541 cccggatgat ttcccgcgaa tgtgggaaag attcgccctg gtcagggtgt ggaatggtgt
      601 ccacactcga ggcaggcatg gcctgggcga agggaggccg tcgtgcgcag acgccttccg
      661 atcgtcttcg ttgtggtcgg ttccctgttg ctggctgccg gtctcgtcgc gggcatcatc
      721 aaccgtgagg tgatcgacgg gtcacgattc gtcgatcatg tcgacgccat ccgtcaggat
      781 cctgacgtct cgcgacaggt cggcctggcg ttgagcgacc gcgtcatcga ggccgacccg
      841 gacctggtcg tggtccggcc attgctcgag tcggcggcgc aggcgttggt ggccgagccg
      901 gcgttcggca tcgtggctcg agcctcagct gcccaattcc acaatgcgtt caccgagtcc
      961 ggtccagtgg tcatccgggt ggccgacgtc ggagccgtgt tgactgcggt gctgtcggcg
     1021 tacgcgccgc aggcatcggc caagctgcct gcgaacctcg acgcgacgtt ggcacgtgtc
     1081 ggcagccaga ccttcgccgc ggacacgatc catctcaccg agaaggtccg gctgctcgct
     1141 tggctgctcc cgctgctggc gctgttggcc ttcgcggtcg ccgtcgtcgt aacgagcaat
     1201 cgtcgccgtg ccatggctgc cgtcggttgg gctgtcttcg ccgcaggcgc cctgctggcc
     1261 tgcctcgggt tcgggctgtg gctcgtcaca tcgttcgccg ataccgacac cttgtcgggc
     1321 gcgctcgtcg tcgccggctg gcgcgagctg cagcagccgg tctggctcac tgcagccggc
     1381 gccatggtgg cgggcgcagt gttcggtgtc gcggtcgccc gaagcggccg ccgtagcgtc
     1441 ccggaaatcc ttgcggccgg ttgggcatgg atgacccgag actccgggcg tcaccacgag
     1501 cgactgctcc gcgcagtggt gtggctgggc atcggtgtcg gtctcgtggt ccggccgctg
     1561 gtcttcgtcg ctgccatcgc ggtggcgctc ggtgtggctg ctgtcgtgac cggagccaac
     1621 gggttgctcg ccgtggcgaa ggagcgcgta tcggtgccac ggcgccgacc gcggttcgcg
     1681 gtcgacccgc ggtggtggag agccgtcggt atcacgctgg gtgtggcatt gctcgtgtcg
     1741 ctcctcgtcg cgttgttcca gccggtcgac gagacgatcg ccgcgggctc ccagtccgcg
     1801 tactccggac cgtgcaacgg ccacgtcgac ctgtgcgtac gccgctacga cgacgtggcc
     1861 tacgcggcca cccacaactc gatggccgcc gcggacgagc ccaactggtt cctcgcggag
     1921 cagccgacgg gcctggtcgg gcagctcgac gacgggatca gagtgctcct gatcgacacg
     1981 tggtacgggc aggccaccca gcgtcgcgac atcgtggcca ctgcatcacg cagccggcaa
     2041 cgcgcgacgg ccgaggccgt cgacaggtac gggcaaagcg tcgtggatgc ggcgctgcga
     2101 ctgcgggacg cggcctcgct cacgcccagc ggaccggtcg agccgtacct ctgtcacagc
     2161 atgtgtgagc tcggctccac caagttcgtc ccggcattgg tgcagatgcg tgcgtggttg
     2221 gacgcccatc cacgcgaggt cgtgaccctg ttcatccagg acgaggtgac cccggccgac
     2281 accgccgaag cgttcgagca ggccggattg ctgtcgtatg tccacaccca gcagaccggt
     2341 gggtcgtggc cgacgttggg ggagatgatc gcgtcgggtc gccggcttgt tgtcttgatg
     2401 gagaaccatg gcggcggctc gacgtacccg tggctgctgc agggcttcga ctgggtgcag
     2461 gacacgccgt acgactacac ctcgccgagc cagttcagct gccggcgcta ccgcggttcc
     2521 gccgccagtc ctcttttcct ga
//
LOCUS       DATBFZ010000299         9798 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_536080, whole genome shotgun sequence.
ACCESSION   DATBFZ010000299 DATBFZ010000000
VERSION     DATBFZ010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 9798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9798
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_536080"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..1467)
                     /locus_tag="VH419_09165"
     CDS             complement(70..1467)
                     /locus_tag="VH419_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013861927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEY7043823.1"
                     /translation="MTIDSSLDSTQLDNAALQQSARDHLWMHFTRMSSYEKADVPIIV
                     RGEGAYIYDANGKRYLDALAGLFVSQLGHGRTELAEAARRQAEQLAFMPLWSYAHPNA
                     IELAQRVASYAPGELNRVFFTSGGGEAVESAWKLAKQYFKLTGKPTKHKVISRTIAYH
                     GTTQGALSITGLPGLKSMFEPLVPSTFRVPNTNIYRAPVHGDDPEAFGRWAADQIAVA
                     IEDEGPETVAAVFLEPVQNSGGCFPPPPGYFQRVREICDEYDVLMVSDEVICAFGRLG
                     HMFGSERYGYQPDMITCAKGITSGYAPLGAMIASDKLMEPFLNSGASFAHGYTFGGHP
                     VSTAVALANLDIFESEKLLENVSDNEAAFKSTLEKLKDLPIVGDVRGSGYFYGIELVK
                     DKNTKETFDDDESEKLLRGFLSKALYDAGLYCRADDRGDPVVQLAPPLICDQEHFDEI
                     EQILRATLTEAWSRL"
     gene            1796..2320
                     /locus_tag="VH419_09170"
     CDS             1796..2320
                     /locus_tag="VH419_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020393164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigE family RNA polymerase sigma factor"
                     /protein_id="HEY7043824.1"
                     /translation="MADRDVTRTSFEQFVAARSGTMLKTAYLLTQDNHLAEDLLQTAL
                     AKAWRSWRRVETSPDAYVRKILVNTYASWWRRKWNGEAPSDLLPEPSDHPDQPDIGAV
                     HDLRNALARLPRRQRAVVVLRFFEDLTEAETARLLECSVGTVKSQTSKALAKLRVDPS
                     LEQHDENADEETSR"
     gene            2317..3330
                     /locus_tag="VH419_09175"
     CDS             2317..3330
                     /locus_tag="VH419_09175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043825.1"
                     /translation="MKVDELKQGLATLAEETENRGIHERLVGIDGKVASHRRAKWIGT
                     GVVAMLAIAAAAIAPGILDTNADRTPAANDDRHAGFNLPTVSDHGTTFYTDPAGARLI
                     GHGVADPGATEVSFSFTPSTLDLAWEGFCWDPTLAAAAQGADYSTFVNGNPVSGSTCQ
                     DSPGPYPVAPTGSFGSSPSQNVNAWADLGVVVGKPSTFTLRITGHAGRSIAPQLGGAV
                     YERGTQQRIEEIWYPQQVVFDDHTYQAVASNVRGFEGRKPSQISIGLPRADHAWYVFR
                     GADHVHGGVNVSAMGHLPAGVPGGGTGDLVPIDDRTFTVTARGLSPDSRGTIYVVVYK
                     RID"
     gene            3419..4423
                     /gene="speB"
                     /locus_tag="VH419_09180"
     CDS             3419..4423
                     /gene="speB"
                     /locus_tag="VH419_09180"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HEY7043826.1"
                     /translation="MTRYGQQFGPDFTFLGVPMADLDDAAGLAAADVVVVGAPFDGGT
                     SHRPGTRFGPMAIRQTDYLGHDGSRPHLALRVDALQDVKVVDVGDVEMAPGEIERALH
                     AVEEAVYAVASSGAVPLVLGGDHSIALPDATGVARHFGFGRVSMIHFDAHADTGDIEF
                     GSLYGHGQPMRRLIESGALRGDRFLQIGLRGYWPGPEVLDWMAGQDMRSYEMTEILAR
                     GLDECLTEAFGIAMDDCDGVFLSVDIDVCDPGHAPGTGTPEPGGLTARQLLDSVRRIC
                     IDLPVVGMDVVEVSPPYDHSDITSYLANRVVLEALSGMAVRKRGGLDHDPAGPLLEGR
                     "
     gene            4570..5751
                     /locus_tag="VH419_09185"
     CDS             4570..5751
                     /locus_tag="VH419_09185"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY7043827.1"
                     /translation="MVPLYAVYALLFGDHGVSTGQISTLFIIWSLTSFAFEIPSGAWA
                     DTIDRRHLLVLSALIYAAGFSCWLLLPSYAGFALGFVLWGLSSAIMSGTFESLLYDEL
                     TERGLESEYTRLIGWAHSTAMLANLAATVLAAPLFALGGYALVGWISVGFAGVQAALA
                     ASLPVSKPARHPNSAHGTADVVWATERFATRYMTMLRAGIREASTSVSVRRVVLIAAG
                     LVGLTAYDEYFPLVARDHGVATGTVPLLIGLVVAGQAVGTGLAGRTARMRPAVMASAV
                     GFGGALISLGALVSPSVGFLTVAIGYGLLNNAMIVAEARLQTAIRGPARATVTSVYGF
                     ATEIFALAVMAPFVLAADVLSVPVLVALMGLVTVGIGGCVARWFPAASSLTPDVVEQA
                     D"
     gene            5756..7399
                     /locus_tag="VH419_09190"
     CDS             5756..7399
                     /locus_tag="VH419_09190"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008358076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEY7043828.1"
                     /translation="MTSHPDLVFTGGAVFTLDDGPPAEAVAVRDGVIVAVGDAGVREL
                     AGPTTEVVDLAGGLLVPGFQDAHVHPVGGGLERLQCDLSENSTREEYLATIRAYADAN
                     PEAKWITGGGWAMAAFPGGTPTAADLDGVVSDRPVFLPNRDHHGAWVNSRALEVAGID
                     GSTPDPVDGVIERDEHGRPTGTLHEGAMHLVGRHAPRSSLDELVAALLEGQRYLHSFG
                     VTAWQDAIVGGYANLDDPSEAYRALVANGRLTAKVVGALWWERDLGAEQIPYLVEQRQ
                     RLTSGRFRATSVKIMQDGVPENFTAHMLDPYLDRAGATTSNRGLPFVDPKALRGYVTE
                     LDKNGFQVHVHAIGDAAVRETLDAFEAAKDANGVQAFAAHRHHIAHIQVIHPDDVSRF
                     AALGVTANMQALWAAYEPQMTELTIPFLGDERAGWQYPFLDLVRSGAPLAMGSDWPVS
                     TPNPLAAIHVAANRVLPDAEGDDRRPFYADQGIDVVTALTAYTAGSARVNHLDDTGTI
                     EVGKAADLAVLDRDIRFVEPETISDTRVLATYVDGHQVF"
     gene            7494..7928
                     /locus_tag="VH419_09195"
     CDS             7494..7928
                     /locus_tag="VH419_09195"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019045037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03667 family PPOX class F420-dependent
                     oxidoreductase"
                     /protein_id="HEY7043829.1"
                     /translation="MTEGPLPDADSDYGFRVRLRLRDEQVIWLTTTSADGTPQPNPVW
                     FLWEDPESLLVYSFHGAQRLKHLQARPRVSLHFNSDAHGGDVVVLVGDASVAEDVPPA
                     HENTAYLAKYDDAMVGVSGTTEAFAATYSVPVRIKVARVRGF"
     gene            complement(8011..9261)
                     /locus_tag="VH419_09200"
     CDS             complement(8011..9261)
                     /locus_tag="VH419_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020576592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY7043830.1"
                     /translation="MPATQPTAPLDLFSIDTLLEDDERAIRDTVRRFCDDRIRPHVAD
                     WYESASLPARDLAKELGALGLMGMHLDGYGCAGTTATAYGLACLELEAADSGIRSLVS
                     VQGSLAMFAIHRYGSDDQKEHWLPQMSAGTAIGCFGLTEPDFGSDPGGMRTRASRDGS
                     DWVINGTKMWITNGAIADVAVVWARTDDPSLRQSSGQALRQGSGQAAGAGQPVIRGFV
                     VPTDTPGFSAPEIKHKMSLRASVTSELVLEDVRLPDSAVLPEAHGLGGPLSCLSEARF
                     GIVFGALGAARECLETTIEYARSRRVFEKSLASYQLTQEKLANMTLELGKGMLLALHL
                     GRLKDSTGVRNEQVSLGKLNNVREALAIARECRTILAANGITLEYPVMRHANNLESVL
                     TYEGTSEVHQLVIGQALTGESAFR"
     gene            9363..>9798
                     /locus_tag="VH419_09205"
     CDS             9363..>9798
                     /locus_tag="VH419_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020662341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY7043831.1"
                     /translation="MATTTTSGGTSGGQTDEFEPEMAVDLRGLTRRFGSVTAVDGIDL
                     SIRDGEFFSMLGPSGSGKTTVLRMIAGFEIPDAGAIWLGGVDVTRRAPFERDVNTVFQ
                     DYALVPHMNVRDNVAYGLRVRGVSKSRRRELAEQALAQVRLDA"
BASE COUNT         1518 a         3292 c         3401 g         1587 t
ORIGIN      
        1 cgtcgccggc ccgagcgcac acaggatgtc gcacacagcg tcagagtctg gacgatgctt
       61 gacggcgcct cagagacgcg accatgcctc ggtgagtgtg gcccgcagga tctgctcgat
      121 ctcgtcgaag tgctcctggt cgcagatcag cggcggagcg agctggacga cggggtcacc
      181 gcggtcgtcg gcgcggcagt agaggccggc gtcgtacagc gccttggaca ggaagccccg
      241 gagcagcttc tccgactcgt cgtcgtcgaa ggtctccttg gtgttcttgt ccttgaccag
      301 ctcgatgccg tagaagtagc cggagccgcg gacgtcgccg acgatcggca ggtccttgag
      361 cttctccagg gtcgacttga acgccgcctc gttgtccgag acgttctcga gcagcttctc
      421 gctctcgaag atgtcgaggt tggccagcgc gacagcggtc gagaccgggt ggccgccgaa
      481 ggtgtagccg tgggcgaagc tcgcgccgct gttgaggaac ggctccatca gcttgtcgct
      541 ggcgatcatc gcgccgagcg gggcgtaccc ggaggtgatg cccttggcgc aggtgatcat
      601 gtccggctgg tagccgtagc gctcggagcc gaacatgtgg ccgagccggc cgaaggcaca
      661 gatgacctcg tcggagacca tcagcacgtc gtactcgtcg cagatctcgc ggacgcgctg
      721 gaagtatccc ggcggtggcg ggaagcagcc accggagttc tggaccggct cgaggaagac
      781 ggccgcgacg gtctcgggcc cttcgtcctc gatggccacc gcgatctggt cggcggccca
      841 gcgaccgaag gcctcggggt cgtcgccgtg gaccggcgcc cggtagatgt tggtgttcgg
      901 gacccggaag gtgctgggca ccaacggctc gaacatggac ttgagacccg gcagaccggt
      961 gatcgacagc gccccctggg tggtgccgtg gtaggcgatg gtgcggctga tgaccttgtg
     1021 cttggtgggc ttgccggtca gcttgaagta ctgcttcgcg agcttccacg cggactcgac
     1081 ggcctcgccg ccgccactgg tgaagaagac ccggttgagc tctcccggcg cataggacgc
     1141 cacccgctgg gcgagctcga tcgcgttcgg gtgcgcgtag gaccacagcg gcatgaacgc
     1201 cagctgctcc gcctgccggc gcgccgcctc ggcgagctcg gtccggccgt gaccgagctg
     1261 gctgacgaac aggccggcca gcgcgtcgag gtaccgcttg ccgttcgcgt cgtagatgta
     1321 ggcgccctcc ccgcggacga tgatggggac gtcggccttc tcgtaggacg acatccgggt
     1381 gaaatgcatc cagaggtggt cgcgggcgga ctgctgcagg gcagcgttgt ccagctgggt
     1441 gctgtcgagg ctggagtcga tcgtcatggc gcccagtctg cctcacggct tccgtcacag
     1501 gcaagtgaat ccgtggtccg ttcgggattc ggcagccctg gtacgccgcg acgccggagt
     1561 tcagcaacga aatccgtcgt tcgaagcgtt tctcggcagc cacaggcaac catcgaggcg
     1621 ggccagccgg tcaatcgagg cgaacgacag ggacggccaa tgaatcacga cagacccggc
     1681 ggtggtcgaa ggaccgcccg gacagccagg aggacacgtg ccaggacggt cggtggtggt
     1741 gccacccgct cgacgagcat cggctgcgga cccgtcggta cccgacgatg gtctcgtggc
     1801 agaccgggac gtgacgcgca cgtcgttcga gcagttcgtc gccgcccgct cgggcacgat
     1861 gctcaagacg gcgtacctgc tcacccagga caaccacctc gcggaggatc tgctgcagac
     1921 ggcgctggcc aaggcctggc gatcgtggcg gcgggtcgag accagccccg acgcgtacgt
     1981 ccggaagatc ctcgtcaaca cctacgcctc ctggtggcgg cgcaagtgga acggcgaggc
     2041 tccgtccgac ctcctgccag agccgtccga ccatcccgac cagcccgaca tcggtgcggt
     2101 ccatgacctc cggaacgcgc tggcccggtt gccgaggcgg cagcgggcgg tcgtggtgct
     2161 caggttcttc gaggacctga cggaggcgga gaccgcccgg ttgctcgagt gcagcgtcgg
     2221 aacggtgaag agccagacga gcaaggcact ggccaagctg cgggtcgacc ccagcctcga
     2281 gcagcacgac gagaacgcgg acgaggagac atcacgatga aggtcgacga gctgaagcaa
     2341 ggcttggcca cgctggccga ggagaccgag aaccgtggca tccacgaacg gttggtcggc
     2401 atcgacggca aagttgcctc gcaccgacgc gccaagtgga ttgggaccgg tgtggtcgcc
     2461 atgctcgcga tcgcagcggc cgcgatcgcg ccggggatcc tggacaccaa cgcggaccgg
     2521 acgcccgcgg cgaacgacga ccgacacgcc ggcttcaacc tgccgacggt ctctgaccac
     2581 ggcacgacgt tctacaccga ccctgcaggc gcgaggctga tcggtcacgg tgtcgccgat
     2641 ccgggggcca ccgaggtctc gttcagcttc acaccgtcga cgctcgacct ggcctgggag
     2701 ggattctgct gggatccgac actggcggcc gctgcgcagg gagcggacta ctcgacgttc
     2761 gtcaacggca acccggtcag cggctccacc tgccaggaca gcccgggtcc gtatccggtc
     2821 gctccgacgg gctcgttcgg cagctcgccg agccagaacg tcaacgcatg ggctgacctg
     2881 ggcgtcgtgg tcgggaagcc gtcgacgttc acgctgcgga tcaccggtca tgcaggcagg
     2941 tcgatcgcgc cgcagctcgg tggggcggtg tacgagagag gcacgcaaca gcgcatcgag
     3001 gaaatctggt atccgcagca ggtggtgttc gacgaccaca cgtaccaggc cgtggccagc
     3061 aacgtgaggg gcttcgaggg gcgaaagccc agtcagatct ccatcggtct cccgcgagca
     3121 gaccacgcgt ggtacgtctt ccgcggcgcg gaccacgtgc acggtggggt gaacgtgtcg
     3181 gcgatgggtc acctccccgc cggcgtccct ggcggtggaa ccggagacct ggtgccgatc
     3241 gacgaccgga ccttcacggt caccgcacgt ggcctgagcc cggacagccg gggcacgatc
     3301 tacgtcgtcg tctacaagcg catcgactga cgcccttccg agttgcactg ccgagttgcg
     3361 cccacgtcag agggcttcct cgcgcccgct gacaactctg gtgcggaagg atgtggccat
     3421 gacccgatac gggcagcagt tcggtccgga cttcacgttc ctcggcgtcc ccatggccga
     3481 cctcgacgac gcggcggggt tggcggcggc cgacgtggtg gtcgtgggcg cgcccttcga
     3541 cggtggtacg tcgcaccggc ccggcacccg cttcgggccg atggcgatcc ggcagaccga
     3601 ctacctcggg catgacggct cccgtccgca cctcgccctg cgggtcgacg cgctccagga
     3661 cgtgaaggtg gtcgacgtcg gcgacgtgga gatggcgccg ggcgagatcg aacgcgcgtt
     3721 gcacgcggtc gaggaggcgg tgtacgccgt cgcgtcctcg ggtgcggtgc cgctcgtcct
     3781 cggcggcgac cactcgatcg cactgccgga cgccaccggt gtggcccgcc acttcggctt
     3841 cgggcgggtg tcgatgatcc acttcgatgc ccatgccgac accggcgaca tcgagttcgg
     3901 ctcgttgtac gggcacggcc agccgatgcg gcggctcatc gagtccgggg ccctgcgggg
     3961 tgaccggttc ctgcagatcg gcctgcgggg gtactggccc ggacccgagg tgctcgactg
     4021 gatggccggg caggacatgc ggtcgtacga gatgaccgag atcctcgctc gcgggctcga
     4081 cgaatgcctg accgaggcgt tcggaatcgc gatggacgac tgcgacggcg tcttcctctc
     4141 ggtcgacatc gacgtctgcg accccgggca cgcgccgggg accgggacac ccgagcccgg
     4201 cggactcacg gcccgacagt tgctggactc ggtacgtcgg atctgcatcg acctcccggt
     4261 cgtcgggatg gacgtggtcg aggtgtcgcc gccgtacgac cattccgaca tcacgtccta
     4321 cctcgcgaac cgggtcgtgc tcgaagcgct gtccggcatg gccgtccgca agcgcggcgg
     4381 cctcgaccac gaccccgccg gcccgctgct cgagggccgc tgagttgtca gaaccgggtt
     4441 gagccataac gccttccagc tctgacaagt cggcgctacc gttggcccat gtcgttcccg
     4501 ccgggccaca cctaagtcct gccgcgcggc ttcacgctgc gcgtcctcgc ctaccacgcg
     4561 tcgcgggaca tggtcccgct gtacgccgtg tacgcgctgc tcttcggaga ccacggtgtc
     4621 tcgaccgggc agatctcgac cttgttcatc atctggtcgc tcacgtcgtt cgcgttcgag
     4681 atcccgtccg gcgcttgggc cgacacgatc gatcgccgcc acctgctcgt cctgtcggcg
     4741 ctgatctacg ccgctggctt cagctgctgg ctgcttcttc cgtcgtacgc agggttcgcc
     4801 ctcggcttcg tgctctgggg tctgtcgagc gcgatcatgt ccggcacgtt cgagtcgctg
     4861 ctgtacgacg agctgaccga gcgtggtctc gagtcggagt acacccggct gatcgggtgg
     4921 gcgcactcga cggcgatgct ggccaacctg gcggccaccg tgctggccgc gccgctgttc
     4981 gccctcggcg ggtacgcgct cgtcgggtgg atctcggtcg gcttcgccgg cgtacaggcg
     5041 gcgctggccg catcgctccc ggtgtcgaag ccggcccggc accccaacag cgcacacggt
     5101 acggcggacg tggtctgggc gacggaacgc ttcgcaacga gatacatgac gatgctgcgc
     5161 gccgggattc gcgaggcatc gacgtctgtg tcggtgcgtc gtgtggtcct catcgctgcc
     5221 ggactcgtcg gtctcaccgc gtatgacgag tacttcccgc tggtggcccg tgatcacgga
     5281 gtcgccacag ggaccgtgcc gctgctcatc ggtctcgtgg tggcagggca agccgtcgga
     5341 actgggctcg cggggaggac ggcgcggatg cggccggcgg tgatggcgtc ggcggtcggg
     5401 ttcggtggcg cgctgatctc gctcggcgcg ctcgtcagcc cgtcggtggg tttcctgacc
     5461 gtcgcgatcg gatacgggct gctcaacaac gcgatgatcg tcgccgaggc gcgactgcag
     5521 acagcgatca ggggtccggc acgcgcgacg gtcacgtcgg tgtacgggtt cgccaccgag
     5581 atcttcgccc tcgccgtcat ggcgcccttc gtcctggcgg ccgatgtgct gtccgttccc
     5641 gtcctggtcg cactcatggg tctcgtgacc gtgggaatcg gcgggtgtgt ggcgcggtgg
     5701 ttcccagcgg ccagtagcct cacgccggac gtcgtcgaac aggcagactg acgccatgac
     5761 ctcacacccg gacctggtct tcaccggtgg cgctgtcttc acgctcgacg acggcccccc
     5821 ggccgaagcc gtggcggtgc gtgacggcgt catcgtggcc gtcggcgatg ccggcgtacg
     5881 agagctcgcc ggtccgacga cagaggtggt cgatctcgcg ggtgggctgt tggtcccggg
     5941 cttccaggat gcgcacgtgc acccggtggg cggtggtctc gagcggttgc agtgtgacct
     6001 ttccgagaac tccacccgcg aggagtacct ggccacgatc cgggcgtacg ccgacgcgaa
     6061 tccggaggcg aagtggatca ccggcggcgg ttgggcaatg gcggcgttcc ctggtggtac
     6121 gccgacggcc gccgacctgg acggcgtcgt gtccgaccga ccggtgttcc tgcccaaccg
     6181 cgaccatcac ggtgcctggg tcaacagccg ggccctcgag gtcgccggca tcgacggctc
     6241 gactccggat ccggtggacg gcgtcatcga gcgcgacgaa cacggccgac ccactgggac
     6301 gctgcacgaa ggagccatgc accttgtcgg tcgtcacgca ccgcgcagca gcctggacga
     6361 gctggtcgca gcccttctgg agggacagcg ctatctgcac tcgttcggtg tcaccgcgtg
     6421 gcaggacgcc atcgtcggcg ggtacgccaa cctcgacgac ccgagcgagg cctaccgtgc
     6481 gctggtggcg aacgggcggc tcaccgcgaa ggtggtcggt gccctgtggt gggagcgtga
     6541 cctcggcgct gagcagatcc cgtatctggt cgaacaacgc cagcggctga cgtcgggacg
     6601 gttccgggcg accagcgtga agatcatgca ggacggtgtc ccggagaact tcacggcgca
     6661 catgctcgac ccgtacctcg accgagccgg cgccacgacg tcgaaccgtg gcctgccgtt
     6721 cgtcgacccg aaggcgctgc gcggctacgt gaccgagctg gacaagaacg ggttccaggt
     6781 ccacgtgcat gccatcggcg acgcggccgt ccgggagaca ctcgacgcgt tcgaggcggc
     6841 caaagacgcg aacggcgtcc aggccttcgc cgcgcatcgc caccacatcg cgcacatcca
     6901 ggtcatccac ccagacgacg tgtcgcggtt cgccgcgctc ggagtgaccg ccaacatgca
     6961 ggcgctctgg gcggcgtacg aaccgcagat gacggagctg acgatcccgt tccttggaga
     7021 cgagcgagcc ggctggcagt acccgttcct cgaccttgtc cgctccggcg cgccgctcgc
     7081 gatgggcagc gactggccgg tctcgacgcc caacccgctg gcggcgatcc acgtcgcggc
     7141 gaaccgggta ctcccggacg ccgaaggaga cgaccggcgg ccgttctacg ccgaccaggg
     7201 gatcgacgtg gtgacggcgc tgacggcgta caccgcgggg tcggcccgag tgaaccacct
     7261 cgacgacacc ggcacgatcg aggtcggcaa ggcggcggac ctggccgtgc tcgaccggga
     7321 catccgtttc gtcgaacccg agaccatctc cgacacccgt gtcctcgcca cctacgtcga
     7381 cggccaccag gtcttctgac ccgccccccg ccgaggtggc aggtttcgtt gccttctgtc
     7441 ggcgtgtcgt gccatgactg ccacctcgat ggcgactgag tagcctggca gggatgaccg
     7501 agggaccact ccccgatgcc gactccgact acggcttccg ggtacggctg cggctccgcg
     7561 acgagcaggt catctggctg acgacgacga gcgccgacgg cacaccgcaa cccaaccccg
     7621 tttggttcct ctgggaagat ccggaatcgc tgctcgtgta cagcttccat ggcgcccagc
     7681 ggctgaagca tctccaggcc cggccgcgcg tgtcgctgca cttcaacagc gacgcccacg
     7741 gcggcgacgt cgtcgtgctc gtcggtgacg cgagcgtggc cgaggacgtg ccgccggcgc
     7801 acgagaacac ggcctacctc gcgaagtacg acgacgcgat ggtgggcgtc agcgggacca
     7861 ccgaggcgtt cgcggcgacg tactccgtgc cggtgcgcat caaggtcgcc cgcgtccgcg
     7921 gcttctgacg aaccatcgag gtggcaatca cggttcatga accggcgtgt ttccaacgaa
     7981 acttgccacc tcgccgggag cgggaggggg tcagcggaag gcggactcgc ccgtgagggc
     8041 ttggccgatg acgagctggt ggacctcgga ggtgccctcg taggtcagca cggactcgag
     8101 gttgttggcg tggcgcatca ccgggtactc cagcgtgatg ccgttggcgg ccaggatcgt
     8161 gcggcactcg cgggcgatcg ccagcgcttc gcgcacgttg ttgagcttgc ccaggctgac
     8221 ctgctcgttg cgtacgccgg tcgagtcctt cagccgaccc agatgcagcg ccagcagcat
     8281 gcccttgccg agctcgagcg tcatgttcgc gagcttctcc tgggtcagct ggtacgacgc
     8341 caacgacttc tcgaacaccc ggcggctgcg cgcgtactcg atcgtcgtct ccagacactc
     8401 gcgggcggca cccaaagcgc cgaacacgat cccgaacctc gcctccgaca ggcacgagag
     8461 tggtccaccg agcccatgcg cctccggaag gaccgccgag tccggcagtc ggacgtcctc
     8521 gagcacgagc tcactggtca cggacgcgcg cagcgacatc ttgtgcttga tctccggcgc
     8581 cgagaagccc ggcgtgtccg tcggcacgac gaacccacgg atcaccggtt ggcccgcccc
     8641 tgccgcctgt cctgagccct gtcgaagggc ttgtcctgag ctctgtcgaa gggatgggtc
     8701 gtcggtacgc gcccacacca ccgcgacatc ggcgatcgcg ccgttcgtga tccacatctt
     8761 cgtgccgttg atcacccagt ccgagccgtc cctgctcgcg cgggtccgca tgccacccgg
     8821 gtcggatccg aagtcgggct cggtcagccc gaagcacccg atcgccgtac cggccgacat
     8881 ctgcgggagc cagtgctcct tctggtcgtc actgccgtac cggtggatcg cgaacatcgc
     8941 cagcgacccc tgcacggaca ccaggctgcg gatgccgctg tccgccgctt cgagctccag
     9001 gcaagccaga ccgtacgccg ttgcggtcgt cccggcgcag ccgtagccgt ccagatgcat
     9061 gcccatcaga ccgagtgctc cgagctcttt cgcaaggtca cgcgcgggca gactggcgga
     9121 ctcgtaccag tcggcgacgt gcgggcggat ccggtcgtcg cagaaccgcc ggacggtgtc
     9181 gcgaatcgcg cgttcgtcgt cctccaggag cgtgtcgatg ctgaagaggt cgaggggcgc
     9241 ggtgggctgg gtagcaggca tcgcttgcca atatagggat tccgaccgca ggtgcgtcgg
     9301 ccgggacgcc atcgcgcaga attcgtgcgc cgacacgcgc cgtgtgagta gggtgcggga
     9361 caatggcgac aacgacgacg agcggcggga ccagcggggg ccaaacggac gagttcgagc
     9421 cggagatggc cgtcgacctg cgcggtctga cgcgtcggtt cggctcggtg accgctgtgg
     9481 acggcatcga tctctcgatc cgcgacggcg agttcttctc gatgctgggc ccgtccggct
     9541 ctggcaagac gaccgtgctc cggatgatcg ccggcttcga gatccccgac gccggtgcga
     9601 tctggctcgg tggggtcgac gtgacgcgac gagcgccgtt cgagcgcgac gtcaacaccg
     9661 tattccagga ctacgccttg gtcccgcaca tgaacgttcg cgacaacgtc gcgtacgggt
     9721 tgcgcgtccg gggtgtctcg aagagccgcc gccgtgagct cgcggagcaa gccctggctc
     9781 aggtacggct cgacgcgc
//
LOCUS       DATBFZ010000300         3466 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_2498575, whole genome shotgun sequence.
ACCESSION   DATBFZ010000300 DATBFZ010000000
VERSION     DATBFZ010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 3466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3466
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2498575"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2782
                     /locus_tag="VH419_09210"
     CDS             <1..2782
                     /locus_tag="VH419_09210"
                     /inference="COORDINATES: protein motif:HMM:NF012211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEY7043832.1"
                     /translation="ISYGARIVAVADSYEVMTAARPYKRPMTAEAARQELTRCAGTHF
                     DPDVVRAFLTISLGGMRRSAGPLAWLAQILLVRPGPVLGRVLGASVGASAAAASVVAM
                     NLPAIAEVPHDSRPVAQSPVEHPSPTSPSTTTTPPSPGRPPAQPTSPHPSPPLVIPTV
                     TPPRPDPQPPTDPTSTTPTPDPGTPTNPLPTHQPPPPIDTKDDALTTDEDASATLDVM
                     ANDVPPDGQTIAVSTLSKPAHGSVSNGQAGVVYTPDVDFNGTDWFVYTVRDDDGTTAT
                     SRVDVTVLPVDDLPTAIDDTATVTEDVANAHVDVLANDTDADGDALTLVATGVPSHGS
                     SHVTPGGKLAYTPAPDFHGQDSVTYTVQDSSGWTATATVTIAVTSENDDPVAVDDSVT
                     VAEDSTATVLDLVGNDSDPDADALTLVSVSGASHGTVRHHTDGSWTYRPDPDFNGAES
                     LVYSVSDGQASASGVVTIVVSPVDDAPVAVDDNASVLEDAINHPIDVLANDSDEDGDV
                     LSVVAVGSPAHGTAAIMSGQVVYTPDADYHGGDTFDYTVQGGTGLTSSGDVTVSVQSV
                     NDAPAAVDDSLTLAEDSSDVPIVMLGNDTDADGDALSVTSVTGAAHGTVTQQPDGSYT
                     YLPEPDYAGSDSLTYTVSDGNGGTATGTVTVTVSPVNDAPATGPDAYDAATTDLIMTV
                     NAANGVLANDVDADGDTLTVVSDDSAAIDINADGSITYVLPLFGVVQTVQYVVTDGTT
                     TATGTAVFTVTLLPTTVTPLFMQPVDTTSIGDLATSPPSGGVTDLDGDGHPGLTIKDS
                     DLHENEPNPSKFQLWEYTVPGGGVTLNGPIKLDLWTQLNHAPPRDLEYAAWVYDCNAG
                     GTCSPITSAVGIRVRPWSTAAGWQERVVTVGTGNGTIAGGHTIRVRLAFHREDVWLAL
                     DSAHPSSLRLTF"
     gene            complement(2792..2980)
                     /locus_tag="VH419_09215"
     CDS             complement(2792..2980)
                     /locus_tag="VH419_09215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043833.1"
                     /translation="MRKWGPPALAVIVAILAVLVPHPWEGGVVTTITKFHGVHVSDVI
                     VLAALAGFLLIWFHRAGE"
     gene            complement(2977..>3466)
                     /locus_tag="VH419_09220"
     CDS             complement(2977..>3466)
                     /locus_tag="VH419_09220"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY7043834.1"
                     /translation="GAAKILDADVPSDVSDLRTSRAAVSVGFANPGTATGAAGIPERD
                     QALTAKYAELHDFLVTSMRAGSLVQAAVYDADHPDVGPVGGGGYSPVTGVAEIAGVGV
                     LPAYRRRGLAAQLTYVLAKHARDRGVTTVFCSAQNDDVARVYAGIGFVRVGTACISEL
                     AG"
BASE COUNT          595 a         1279 c         1122 g          470 t
ORIGIN      
        1 gatcagctac ggcgcccgga tcgtggccgt ggccgacagc tacgaggtca tgacggccgc
       61 tcgcccgtac aagcgaccga tgaccgccga ggccgcgcgt caggagctga cccgctgcgc
      121 cgggacgcac ttcgacccgg acgtggtgcg cgcgttcctc accatctcgt tgggaggaat
      181 gcgccggtcc gccggtccgc tcgcgtggct ggcgcagatc ttgctggtcc gaccgggtcc
      241 ggtgctcggc cgagtcctcg gtgcttcggt cggggcgtca gcggcggctg ccagcgtggt
      301 cgcgatgaac ctcccggcca tcgcggaggt cccgcacgac tcccgccctg tcgcccagtc
      361 gcccgtcgag catccttcac ccacatcccc gtcgaccacc accacgccac cgagtccggg
      421 ccgcccgccg gctcagccga ccagcccgca tccctcacct cccctggtca ttcccacggt
      481 gacaccgcca cggcctgatc cgcagccgcc gaccgacccg acgtcgacga cacccacgcc
      541 ggaccccggg acacccacca acccgctccc gacccatcag cctccgcccc cgatcgacac
      601 caaggacgac gcgctgacga ccgacgagga cgcgtccgca acgctcgacg tgatggcgaa
      661 cgacgtgcct cctgacggcc agaccatcgc cgtgagcacg ctgtcgaaac ccgcgcacgg
      721 ctctgtctcg aacggccagg cgggtgtcgt ctacaccccc gacgtggact tcaacggcac
      781 cgactggttc gtgtacaccg ttcgggacga cgacgggacg acggccacca gccgggtcga
      841 cgtcaccgtg ctgcccgtgg acgacctgcc gacggcgatc gacgacacgg ccaccgtcac
      901 cgaggacgtg gcgaacgccc acgtcgacgt gcttgccaac gacaccgatg ccgacggcga
      961 cgcgctcacg ctggttgcca ccggcgtgcc gtcgcacggc agctcccacg tcacacccgg
     1021 cggaaagctc gcgtacaccc ctgcacctga cttccacggc caggacagcg tcacgtacac
     1081 cgtgcaggac agctccggat ggaccgcaac cgccacggtc acgattgcgg tcacgtcgga
     1141 gaacgacgac ccggtcgccg tcgacgactc cgtgacggtc gccgaggact cgacagccac
     1201 ggtgctcgac ctggtgggca acgacagtga cccggacgcc gatgccctga ccttggtgtc
     1261 ggtgtcgggc gcctcgcacg gcacggtcag gcaccacacc gacggctcct ggacgtaccg
     1321 gcccgatccg gacttcaacg gcgccgagtc cctcgtctac tcggtgtcgg acggtcaggc
     1381 cagtgccagc ggcgtcgtca cgatcgtggt gtcacccgtg gacgacgcgc cggtcgcggt
     1441 cgacgacaac gcgtcggtcc tcgaggacgc gatcaaccac ccgatcgacg tgctggccaa
     1501 cgacagcgac gaggacgggg acgtgctctc cgtggtcgcc gtcggaagtc cggcccacgg
     1561 cacggccgcg atcatgagcg gccaggtcgt ctacacgcca gacgccgact atcacggcgg
     1621 cgacaccttc gactacacgg tccaaggcgg tactgggttg acctcctccg gagacgtcac
     1681 cgtgtcggtc cagtcggtca acgacgctcc ggccgcagtc gacgactcgc tcacgctggc
     1741 ggaggactcg tccgacgtac cgatcgtcat gctcggcaac gacaccgatg ccgacggtga
     1801 cgctctgtcc gtgacgtctg tgacaggggc ggcgcacgga accgtcacgc agcagccgga
     1861 cgggtcgtac acgtacctgc cggagccgga ctacgcggga tccgactcgc tgacctacac
     1921 cgtgtccgac ggcaacggcg gcacggcgac aggaacggtc accgtgacgg tcagtccggt
     1981 gaacgacgcc ccggccacgg gacccgacgc ctacgacgca gcgaccactg acctcatcat
     2041 gaccgtcaat gcagcgaacg gagtcctggc caacgacgtc gacgccgacg gggacacgct
     2101 gaccgtcgtg agcgacgact cggcggccat cgacatcaac gcagacggat ccatcacgta
     2161 cgtgctcccg ttgttcggag tggtccagac cgtgcagtac gtcgtgacgg acggcacgac
     2221 gacggccacc ggcaccgccg tgttcacggt cacgctcctg ccgaccacgg tgaccccgtt
     2281 gttcatgcag ccggtcgaca cgacatcgat cggtgacctg gcgacgtccc cgccgagcgg
     2341 cggcgtcacc gatctcgacg gggacggaca tcccggtctc accatcaagg acagcgatct
     2401 ccacgagaac gagccgaacc ccagcaagtt ccagctctgg gagtacacgg tgccgggtgg
     2461 cggtgtgaca ctgaacggac cgatcaagct ggatctctgg acgcagctga accacgcgcc
     2521 accgcgcgac ctcgagtacg ccgcctgggt gtacgactgc aacgctggcg gcacctgcag
     2581 cccgatcaca tcggcggtgg gcatccgggt gaggccgtgg agcaccgcag ccggctggca
     2641 ggaacgggtg gtcacggtgg gcaccgggaa cggcaccatc gccggcggtc acaccatccg
     2701 ggtcagactc gccttccacc gcgaagacgt ctggctggca ctcgactccg cccatccgtc
     2761 cagcctgcgt ctcacgttct gactgtcgcc ctcattcgcc ggcccggtgg aaccagatca
     2821 gcaggaaccc ggccagcgcc gccagcacga tcacgtcgga cacatgcacg ccgtgaaact
     2881 tggtgatcgt ggtgacgacg ccgccctccc acgggtgcgg caccaagacc gcgagaatcg
     2941 ccacgatgac cgcgagcgca ggcggccccc acttcctcac ccggcgagct cggagatgca
     3001 ggcggtgccg acccggacga agccgatccc ggcatacacc cgcgcgacgt cgtcgttctg
     3061 ggcggagcag aagaccgtcg tgacgccacg gtcgcgcgcg tgcttcgcca gcacgtaggt
     3121 gagctgggcg gcgaggccgc gtcggcggta tgcggggagc acgccgaccc cggcgatctc
     3181 ggcaacgccg gtgacggggg agtagccgcc gccgccgacc gggccgacat cggggtggtc
     3241 tgcgtcgtaa accgcggcct ggacgaggct tccggcgcgc atcgacgtga ccaggaagtc
     3301 gtggagctcg gcatacttcg cggtcagtgc ctggtcgcgc tcagggatcc ccgcggctcc
     3361 cgtcgccgtt cccgggttgg cgaacccgac ggacacggcc gctcgtgacg tgcgcaagtc
     3421 gctgacgtcc gagggaacgt cggcgtcgag gatcttcgca gcgccg
//
LOCUS       DATBFZ010000301         3383 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_5356148, whole genome shotgun sequence.
ACCESSION   DATBFZ010000301 DATBFZ010000000
VERSION     DATBFZ010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 3383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3383
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5356148"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1149)
                     /locus_tag="VH419_09225"
     CDS             complement(<1..1149)
                     /locus_tag="VH419_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020386056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY7043835.1"
                     /translation="MRVTDLDDRWQANYRRGQDGKDAITGRHDMGSYRETYDASIHEP
                     DTFWRDAAGLVDWFAKPSTVLDGSRAPFYRWYPDGTLNTCYNALDRHVVKGRADQTAL
                     IYDSPVTGTVSSYTYAELLEKVAAFAGGLRGLGVEKGDRVVIYMPMIPEAVIAMLACA
                     RLGAVHSVVFGGFAANELAVRIDDATPTVIVSASCGIEPSRVVEYKPLLDRALTIAAH
                     SPRAVVVKQREQCSAAMDEQRDVDFDTLLTAGRSDPASCVEVAATDPLYILYTSGTTG
                     KPKGIVRDNGGHAVALAWSMANIYDVTPGQVWWTASDVGWVVGHSYIVYAPLIIGATT
                     VLFEGKPVGTPDAGTFWRVVADHRVEGLFTAPTAIRAIKKEDPDGDHIG"
     gene            1322..2188
                     /locus_tag="VH419_09230"
     CDS             1322..2188
                     /locus_tag="VH419_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020387782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIO1 family regulatory kinase/ATPase"
                     /protein_id="HEY7043836.1"
                     /translation="MHVSDEVTSDFSTSIPSYDFSQLTFHTQAELGVDQRWSTWSSVE
                     KGAHGPEPRPDWVVTEDAAVDTELGILKTGKEGDVFLLERAVPDDPSRRSVMAAKRYR
                     DNDHRQFHRDAGYVEGRRIRKSREARAAARGTAFGRAVQAVEWANHEFSALCALWSAG
                     VPVPYPVQIDGTEILMEFIDVDGAAAPRLAQARPDRATLESYFEQLRDAMSILARRHV
                     AHGDLSAYNILAQGDRIVIIDLPQVIDIVSNHNGVEFLLRDCRNVCAWFSSRGLDVDP
                     DELLAELLAQAW"
     gene            2247..2723
                     /locus_tag="VH419_09235"
     CDS             2247..2723
                     /locus_tag="VH419_09235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043837.1"
                     /translation="MARAGVHRAGLLLATALVTAGCGGDSSEDLTTDEAAQQYEQLVT
                     PFNDALSDLQEELKSTPIDLAEVSDAAGRYVAEAKELQTGLKGQDWPDDVSADVVDLS
                     AALERMIPAGQSLVDDSSVDELTTDLQELAAAVDEGTASAGAIRTKLGLEDAPTLS"
     gene            complement(2767..>3383)
                     /locus_tag="VH419_09240"
     CDS             complement(2767..>3383)
                     /locus_tag="VH419_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020387664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043838.1"
                     /translation="GLPRSHASIQGNALGSCCRCGLGRDPRADQLAAALVSWQWPDGG
                     WNCDARSGGYRSSFHETTGAAWGLFEYAEATGNSAAREAADRACELFLEHRVFRRLGD
                     GDVIHPMWLRLRYPPYWHYGILPALILLTRLGKVRDPRAADALDELEKRRLSDGRWAS
                     QGQWWKPTGSSVTPEVVDWGGSGRPNPMLTFHALRILQAAGRLD"
BASE COUNT          553 a         1156 c         1131 g          543 t
ORIGIN      
        1 gccgatgtgg tcgccgtcgg ggtcctcctt cttgatcgcc ctgatcgccg ttggtgcggt
       61 gaacaggccc tcgacccgat ggtcggcgac cacgcgccag aacgtccccg cgtccggcgt
      121 cccgaccggc ttgccttcga agagcacggt ggtggcgccg atgatcagcg gtgcgtagac
      181 gatgtaggag tgcccgacga cccagcccac gtcggaggcg gtccaccaca cctggccagg
      241 ggtgacgtcg tagatgttcg ccatcgacca ggcgagcgcg acggcatgtc cgccgttgtc
      301 gcggacgatt cccttcggct ttcccgtcgt ccccgacgtg tacaggatgt acagcgggtc
      361 ggtcgccgcc acctcgacac acgaggccgg atccgagcgc cccgccgtga gcaacgtgtc
      421 gaagtcgacg tcgcgctgct cgtccatcgc agcactgcac tgctcgcgtt gcttcacgac
      481 gacggcgcga ggactgtgcg cggcgatcgt gagggctcgg tcgagaaggg gtttgtactc
      541 gacgacccgt gagggttcga tcccgcacga tgcggacacg atcacggtcg gtgtggcgtc
      601 gtcgatccgg accgcgagct cgttcgcagc gaagccaccg aacaccacgg agtggacggc
      661 cccgaggcgc gcgcaggcga gcatcgcgat gaccgcctcg gggatcatcg gcatgtagat
      721 gacgacccgg tcgcctttct cgacgccgag accgcgcagg cctccggcga acgcggcgac
      781 cttttcgagc agctcggcgt acgtgtagga cgacacggtt ccggtgacgg ggctgtcgta
      841 gatcagcgcg gtctgatcgg cccggccctt gaccacgtgt cggtcgagcg cgttgtagca
      901 ggtgttgagc gtgccgtcgg gataccagcg gtagaacgga gcgcgcgacc cgtcgaggac
      961 cgtggacggc ttcgcgaacc agtcgaccag gccggccgca tcacgccaga acgtgtccgg
     1021 ctcatggatg ctggcgtcgt acgtctcgcg atagctgccc atgtcgtgcc tcccggtgat
     1081 ggcgtccttc ccatcctgcc cccgccggta attcgcttgc caccggtcgt cgaggtctgt
     1141 caccctcacg ggacctggtg tacgacgggc cggctgttgg ctgcccgtcc tcgagcgaag
     1201 gaggcgtcga catgtcaacc ccggtcctgg ggcagcactt ctgcgtccct tcacctacga
     1261 ggtctccgct tcgctgaacg tggtcggcgg cctcggcacc gctcgaggag caaccaccac
     1321 catgcacgtc tctgacgagg tcacgtccga cttcagcaca agcattccca gctacgactt
     1381 ctcacaactc accttccaca cccaggccga gctcggcgtg gaccaacgct ggtccacctg
     1441 gtcgtcggtg gagaagggcg cgcacggtcc tgagccacga cccgactggg tggtgaccga
     1501 ggacgccgcc gtcgacaccg agctcggcat cctcaagacc ggcaaggagg gcgacgtctt
     1561 cctgctcgaa cgcgccgtcc ccgacgaccc gagccgacga tccgtgatgg cagccaagcg
     1621 ctatcgcgac aacgaccacc ggcagttcca ccgcgacgcg gggtacgtcg aagggcgccg
     1681 catccgcaag agccgggagg cgcgcgcagc ggcgagaggc acggcgttcg gccgggcggt
     1741 ccaagctgtc gagtgggcca accacgagtt cagcgcattg tgtgccctgt ggtcggcggg
     1801 cgtcccggtc ccctaccccg tgcagatcga cggcaccgag atcctgatgg agttcatcga
     1861 cgtcgacggc gctgccgctc cgaggctcgc ccaggcgcgt ccggaccgtg caacgctcga
     1921 gtcgtacttc gagcagctcc gtgacgccat gtcgatcctg gcccgtcgac acgtggcgca
     1981 cggcgacctg tcggcgtaca acatcttggc tcagggggat cggatcgtga tcatcgatct
     2041 cccccaggtg atcgacatcg tcagcaacca caacggtgtc gagttcctgc tgcgcgactg
     2101 ccgcaacgtc tgcgcgtggt tcagctcccg tggcctcgac gtcgacccgg acgaattgct
     2161 cgccgagctc ctcgcccagg cctggtgagg aaacgagcag gcttctgccg gagtaggttc
     2221 ggagcaccgg atttctggag gcggccatgg ctcgcgctgg ggtacatcga gcgggcttgc
     2281 tgctcgcgac cgcgctggtg accgcgggtt gtgggggtga ctcgtccgaa gacctcacca
     2341 ccgacgaggc cgcccagcag tacgagcagc tggtgacgcc gttcaacgac gctctgtccg
     2401 accttcagga agagctgaag tccactccga tcgatcttgc cgaggtcagc gacgcggcgg
     2461 gacggtatgt cgcggaggcc aaggagctgc agaccgggct gaagggtcag gactggccgg
     2521 acgacgtctc cgccgacgtg gtcgacctgt ctgcggcgct cgagaggatg atcccggcgg
     2581 gacagtcgct ggttgacgac agctcggtcg acgagctcac caccgacctg caggagctgg
     2641 cagccgctgt cgacgagggg accgcctccg ccggcgcgat ccgcacgaag ctcggtctcg
     2701 aggacgctcc gaccttgtca tagtcgctcc cagtcgatct cagtcagtct cagtcgcggc
     2761 gtagcgtcag tcgaggcgac cggccgcctg caggatgcgc agcgcatgaa acgtcagcat
     2821 cgggttcggc ctgccggagc cgccccagtc gacgacctcg ggggtcactg agctcccagt
     2881 cggcttccac cactgtcctt gggatgccca tcgcccgtcc gacagccgtc gcttctccag
     2941 ctcgtcgagc gcatcggcag ctcgcggatc gcggaccttg cccaaccggg tgaggaggat
     3001 cagcgcagga aggatgccgt agtgccagta cggcgggtag cgcagtcgga gccacatcgg
     3061 gtggatgacg tcgccgtcgc cgagccgcct gaacacccgg tgttcgagga agagctcgca
     3121 ggcccgatcg gccgcttcgc gggcagcaga gttgccggtc gcctcggcgt actcgaacag
     3181 accccaggct gccccggtgg tctcgtggaa cgacgagcga tacccacccg agcgtgcgtc
     3241 acagttccac cctccgtccg gccactgcca cgacaccaga gccgcggcca gctggtcagc
     3301 ccgggggtca cggcccaacc cgcagcgaca acaggagccc agcgcgttcc cctggatcga
     3361 ggcatgggag cgcggcagcc cgt
//
LOCUS       DATBFZ010000302         3971 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_537131, whole genome shotgun sequence.
ACCESSION   DATBFZ010000302 DATBFZ010000000
VERSION     DATBFZ010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 3971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3971
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_537131"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="VH419_09245"
     CDS             <1..418
                     /locus_tag="VH419_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020390818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEY7043839.1"
                     /translation="EAALGPLRKALGVWVRQHAPYLEARARLLLAECCRGLGDDESGD
                     RELAASRAIFRDLGAAPDLARVDAVQDATGDGKLTVRELEVLRLLATGSTNKAIAASL
                     VLSERTVDRHVSNIYMKLGVSTRAAATAYAFERHLV"
     gene            526..1623
                     /locus_tag="VH419_09250"
     CDS             526..1623
                     /locus_tag="VH419_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008359501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEY7043840.1"
                     /translation="MSETFGTVVIGAGQAGLSAGYHLARLGRPYVILEGDDRVGGSWL
                     HRWDSMRLFTPATHDGLPGMKFPKGSRFPTGREMADYLAEYAERNALAVRTGVRVDGL
                     FREGDGFRVTAGATAYDADNVIIATGVHRRPRVPDFASALSPDIVQLHSADYRNPGQL
                     QPGTVLVVGAGNSGADIGIEVAQTHHTLLAGRHPGQIPIDIESRQARMIFPVLWFVWT
                     HVLTEKSKVGRKVQAERLEGHGDPLIRLKPEDIDRAGIERTERITGVVDGLPQTADGA
                     VLEVRNVIWATGFLHDFSWIDLPGLDASHRLPNDRGRVDGQPGLYVLGQEFQYMFNSH
                     TVGGVGRDAAYVVGDLSRRPARSHEAVVTAH"
     gene            complement(1610..2446)
                     /locus_tag="VH419_09255"
     CDS             complement(1610..2446)
                     /locus_tag="VH419_09255"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY7043841.1"
                     /translation="MRIEVSGTHLWFDTAGSSLVPDTDSMRQRPTFVLVHGGPGVYDH
                     SYFKPYFDTLAEHAQVVYLDLRGHGRSDWGDTGAWTLETCADDVHVFCETLGIERPFV
                     LGHSMGGPIVLLYGARHPGQAGGLIVSSGFARWDHDRLVEGFRRVAGDEVADLADRSY
                     LDEDVADDEWARVYAAFGRHVPGEEKARTPQNLDLNTHGMNLIRRLDIVDQLERVDVP
                     TLVSVGELDPVTPVAAAEEIVAALPDGLGRLQVIPEAGHFTWLDAPDRYWQMLVDFVS
                     VR"
     gene            complement(2459..3379)
                     /locus_tag="VH419_09260"
     CDS             complement(2459..3379)
                     /locus_tag="VH419_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3500 domain-containing protein"
                     /protein_id="HEY7043842.1"
                     /translation="MEKVALEFLGSLDDRQRAVATYPFDSDERFRWQYTPGPRGGLAL
                     ADMDDEQRGLALTLIEAGLSRRGAETARQIMQLEPILRDIEERASRPGADRRDPQFYW
                     FSVFGDPDGSQPWAWRVGGHHLCLHFTVVGAKVSVTPLFFGANPARVPDGPKAGLRTL
                     ADEEDLARQLVASLDETQRHTAVVSTSAPADILTRNQVRAEIAAVPTGIEYDALGSAQ
                     QATLKRLLEVYTGRVDMPSNVVPEVLRFAWAGSTAPGKGHYYALRSDIVLIEYDNTQD
                     GANHIHTVWRDLRRDWGTDLLAEHYRDAHA"
BASE COUNT          653 a         1356 c         1340 g          622 t
ORIGIN      
        1 ggaggcagcg ctcggcccgc tccgcaaggc ccttggtgtc tgggtgcgac agcacgcgcc
       61 gtacctcgaa gcgcgcgcgc gactgctgct ggcggagtgc tgccgaggac tcggggacga
      121 cgagtcgggc gatcgcgagc tggcggcgag tcgggcgatc ttccgcgacc tcggagcggc
      181 tccagacctc gcgcgcgtcg acgctgtcca agacgccacc ggtgacggga agctcaccgt
      241 gcgggagctc gaagtgcttc gcctgttggc gacaggctcg acgaacaagg caatcgccgc
      301 gagcttggtg ctcagtgaac gcaccgtgga ccggcacgtg agcaacatct acatgaagct
      361 cggcgtctcg acgcgcgctg ccgcgacggc gtacgcgttc gagcggcacc tcgtctgagc
      421 cggtacccac agctggccga ccaccggttg ggtggtttca ccgacgcggg gcagcaacct
      481 gccgccctac cttcgtagcg ctcgacatgc gacggaggag gaaccatgag cgaaacgttc
      541 gggacagtgg tgatcggagc gggccaggcc ggactctcgg cgggctacca cctggccagg
      601 ctgggccggc catacgtgat cttggagggc gacgaccggg tcggcgggtc ctggctgcac
      661 cggtgggact ccatgcggtt gttcactccc gccacccacg atggccttcc cggcatgaag
      721 ttcccgaagg gaagtcgctt ccccaccggg cgcgagatgg ccgactacct ggcggagtac
      781 gccgaacgca acgcgctggc cgtgcgtacc ggagttcgag tcgacgggct gttccgagag
      841 ggcgacggct tccgggtcac ggcgggcgcg acggcatacg acgccgacaa cgtcatcatc
      901 gccaccggcg tccaccgccg accccgcgtc cccgactttg cgtccgcact ctcgcctgac
      961 atcgtgcagc tgcactccgc tgactaccga aaccccggtc agctgcagcc cggcaccgtc
     1021 ctcgtggtcg gagccggcaa ctccggcgcc gacatcggca tcgaagtcgc gcagacgcac
     1081 cacaccctgc tggcaggccg ccacccggga cagattccga tcgacatcga gtcacggcag
     1141 gcgcggatga tcttcccggt cctgtggttc gtctggacgc acgtgctcac ggagaagtcg
     1201 aaggtgggtc gaaaggttca ggccgagcgg ctcgagggcc acggcgaccc gctgatccgg
     1261 ttgaagcccg aggacatcga ccgtgcgggc atcgagcgca cggaacggat caccggcgtc
     1321 gtcgacggtt tgccgcagac agcggacggc gccgtcctgg aagtgcgcaa cgtcatctgg
     1381 gcaacggggt tcctgcacga cttcagctgg atcgaccttc ccggcctgga cgcgtcgcat
     1441 cggctgccga acgaccgcgg ccgcgtcgac gggcagccgg ggctctacgt gctcggccag
     1501 gagttccagt acatgttcaa ctcccacacc gtcggtgggg tcggccgcga tgccgcatac
     1561 gtcgtaggcg acctgtcccg tcgtcccgcc cgcagccacg aggccgtcgt caccgcacac
     1621 tgacgaagtc cacgagcatc tgccagtagc ggtcaggggc atccagccag gtgaagtggc
     1681 cggcttcggg gatcacctgg agccggccca ggccgtcggg tagggcagcc acgatctcct
     1741 cggccgcggc gaccggtgtc acagggtcga gctcgccgac cgagaccagg gtgggcacgt
     1801 cgacgcgttc gagctggtcg acgatgtcga gtcggcggat gaggttcatc ccgtgcgtgt
     1861 tgaggtcgag gttctgcgga gttcgggcct tctcctcccc ggggacgtgt cggccgaagg
     1921 cggcgtacac ccgtgcccac tcatcgtccg ccacgtcctc gtcgagatag cttcgatcgg
     1981 cgaggtcggc gacctcgtca cctgcgaccc gacgaaaccc ctcgacgagc cggtcatggt
     2041 cccagcgcgc gaaccccgac gagacgatga gcccgcccgc ctgtccggga tgacgggcgc
     2101 cgtacagcaa cacgatgggg ccacccatcg agtgcccgag gacgaacggc cgctcgatgc
     2161 cgagcgtctc gcagaacacg tggacgtcgt cagcacacgt ctcgagggtc cacgcacccg
     2221 tgtcgcccca gtcggagcgg ccgtgcccgc gcaggtcgag gtagacgacc tgtgcatgct
     2281 cggcgagcgt gtcgaagtac ggcttgaagt acgagtggtc gtacacaccc gggccaccgt
     2341 gcaccaagac aaacgtcggt cgttgtcgca tcgagtccgt gtccggcacg agtgacgacc
     2401 ctgcagtgtc gaaccacagg tgggtgccgc tcacttcgat gcgcatcgcg aacccctctc
     2461 aagcatgggc gtctcgatag tgctcggcca gcaggtccgt gccccagtca cggcgcagat
     2521 cgcgccatac ggtgtggatg tggttcgcgc cgtcctgcgt gttgtcgtac tcgatgagga
     2581 cgatgtcgct gcgcagcgcg tagtagtggc ctttgccggg agccgttgag ccggcccagg
     2641 cgaagcgcag cacctcgggg acgacgttgg acggcatgtc gacccgtccc gtgtacacct
     2701 cgagcaggcg tttcagggtc gcctgctgtg cgctgccgag agcgtcgtac tcgatgccgg
     2761 tgggaacggc cgcgatctcc gcacgcacct ggtttctcgt caagatgtcg gccggcgccg
     2821 acgtcgacac cacagcggtg tgccgctgcg tctcgtccag gctcgccacg agttgacggg
     2881 cgaggtcctc ctcgtcggcc aacgtccgaa ggcccgcctt cggcccgtcg ggcacgcgcg
     2941 ccgggttggc gccgaagaag agcggcgtca ccgacacctt cgccccgacg accgtgaagt
     3001 gcaggcagag atggtgcccg cccacgcgcc acgcccacgg ctgcgaacca tccggatcac
     3061 cgaagacgct gaaccagtag aactgaggat cgcgacggtc ggcccctggc cgcgaggccc
     3121 gctcctcgat gtcgcgcagg atcggctcga gctgcatgat ctgtcgcgcc gtctcggcac
     3181 cgcgacgact cagacccgcc tcgatcagcg tcagggcgag tccgcgctgc tcgtcgtcca
     3241 tgtccgccag cgcgagcccg ccacgtgggc cgggcgtgta ctgccagcgg aacctctcgt
     3301 cgctgtcgaa cgggtatgtc gccacagccc gctgtcggtc atcaagtgag cccaagaact
     3361 cgagcgcgac cttctccatc caccccattc aacacggtga accgtcgcgg ctggagtgtg
     3421 atcgtgacgg gtcatgcaga acggctggac gatcgacacg aggtggaggc catcgcggcg
     3481 tcgcacgacc cgatccctgg gccggtggtc tgcgccgggt gtacttccgc atcccgtgga
     3541 cacacatcag cggacggctc gtcggtgcgg agcggctgca ggcacccacc gtgccggtgg
     3601 ccgaccgctg gcgcagcatc gaaccctgga gcatcggatg accgggccac gataatcgga
     3661 ctccggcgct ccggcggtcg agcggtcgag ccggctgcgg cggtcagcgg gctccgacac
     3721 cccggcggtc gagcttgtcg agacctcgtc tgacagcaac ccttgatctc gacgggtcga
     3781 tcagcgagct cctgcggccc cggcggtcga gcttgtcgag acctcgactg acagcaaccc
     3841 gtgatctcga caggctccat cagcgagctc cgacgactcg gcggtcgagc tcgtcgaaat
     3901 caaggagctt cggcgttgat gtccacagtt catgatgctc agatcccttg tccacaagga
     3961 gtttcggcgg t
//
LOCUS       DATBFZ010000303         5395 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_1518181, whole genome shotgun sequence.
ACCESSION   DATBFZ010000303 DATBFZ010000000
VERSION     DATBFZ010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 5395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5395
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1518181"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="VH419_09265"
     CDS             <1..278
                     /locus_tag="VH419_09265"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY7043843.1"
                     /translation="QAPGRTWIECSWYFPDTGDETPIDASYAVEFWDLTNRQDWAACE
                     SVQRGLASPHFRAGPFSPNEDAVHMFVTTIARAYRGQPIHTPTGTSN"
     gene            complement(783..2741)
                     /locus_tag="VH419_09270"
     CDS             complement(783..2741)
                     /locus_tag="VH419_09270"
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEY7043844.1"
                     /translation="MSTPDVDPRCREVLIVMDDGVRLSATLYLPERDDGPQPCILEAL
                     PYRKDDLTSSYRPDYVRFRDDHGYAVCRLDLRGTGSSGGDATDEYPAREQQDLHQVID
                     WLANQDWCDGNVGMYGTSYSGFNSLQMACERPEHLKAIIAIYGTDDRYTDDVHYRGGI
                     LKLLDLVDYCHYMTPMNVLPPVPAIWGDGWRDEWVRRIETSEPWALTWLREQLDGPYW
                     RHGSVRPGYERIACPTMIIAGWADGYRNNSFRTIERLRAQGVPHRLLAGPWSHASTDT
                     ALPGPRIDSMPEMVAWWDRWLRGIDNGVDDGVSAERPAVTCFVRSSTRPSPVLDESAG
                     FWIREEWPSPRVEVVSRDLAARAAHDVRPDVGVDAWIDCAGHLPWGQSADQRFDDAAS
                     VVWEWDADSVTLVGHPRARLRISVDQPTAYVSVKVCDVFPDGTSALVSRGTLNLGCRD
                     GLDRLVPLEPGQVYDVEVELEACAYAFDPGQRLRLSLAGSDWPNTAAPPRPVTITVPG
                     GELDLPVWTGPSPFAEPDLAPGRTGGEDPTGLTWSVERDVLAGTTSCVVDHGSTYEIP
                     YGGTATEHYEGLVCVEHATFEQRSTATTTFTIRWPDVEVSTTATMEVLIGADAFDVSI
                     EVTAREATEDGFSTVGARQWKQRLPRLA"
     gene            complement(2738..3838)
                     /locus_tag="VH419_09275"
     CDS             complement(2738..3838)
                     /locus_tag="VH419_09275"
                     /inference="COORDINATES: protein motif:HMM:NF015535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HEY7043845.1"
                     /translation="MPRATDLGISIGTCPAGPTNSVLDVEGVGVGHATVWRDEPDPPK
                     GRGVARTGVTVVRLRDDPFHEMLPAGGAVLNGAGECTGFITAGEWGSLETPVFLTSTL
                     QLGRVYDAACELMLERFPDVAEDFIIPVVAECDDSFLNDARRMQVTRHDVKAAWAAAD
                     ASVGSGEPPAEGAVGSGTGMSCLGFKGGIGTASRVTEDGHTVGVLLMTNFGVRDQLTV
                     GGVPVGRLLGTASEHEADADRKPPAGSCIGLVVTDAPVDGAGCARLARRIGLGLARTG
                     SVAHHGSGEIFMATSTGVRVSRDDQTSSTPVVIGRGLDPLFAAVVEASEEAVLNSIFA
                     APTVVGRSGNTSHGVPVDDVVKLLEQHGRITS"
     gene            3877..4941
                     /locus_tag="VH419_09280"
     CDS             3877..4941
                     /locus_tag="VH419_09280"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043846.1"
                     /translation="MPAASDVPVVWSQTTLGHRPDREVWIGMPMEGSELPERVTVIEK
                     ALREAGHPFRDAQPHPVETLGSVHSDEFVRHLSTVYEAWVDGGFVSYGQDRVIPYFFP
                     TDAMLGPISPTPATSVHAAAGRFCYDTMTTVGPGSWAAIKAAADCALTAADLVRSGEA
                     RRVYALCRPPGHHVTADAYGGSCYVNNAALAAQALVQAGHQQVAVIDVDAHHGNGTAA
                     IFYDRPDVTYGSVHVDPAAGWFPHVVGHAAETGRGAGTGTTLNIPLSPGSSDTEFLDG
                     VRRLASHAMTRGATALVVSLGVDAAVNDPESPLEVTEDGYGRAGAILLGELDLPTVVV
                     QEGGYHLPTLGPLVAAFLSR"
     gene            5036..>5395
                     /locus_tag="VH419_09285"
     CDS             5036..>5395
                     /locus_tag="VH419_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009080775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEY7043847.1"
                     /translation="MSVPSTENASSFDSTITLTVNGVVHNLTIDNRTTLLDLLREHLD
                     LTGSKKGCDHGQCGACTVLVAGRRVNACLLLAVACNGRHVTTIEGLGDGGTLHPLQAA
                     FVTHDGLQCGYCTPGQIC"
BASE COUNT          864 a         1953 c         1767 g          811 t
ORIGIN      
        1 cgcaggctcc gggcaggacc tggatcgagt gctcgtggta cttccccgac accggcgacg
       61 agacgccgat cgacgcgtcg tacgccgttg agttctggga cctcacgaac aggcaggact
      121 gggcggcctg tgagtcggtc cagcgtggtc tcgcgtcacc gcacttccgg gccggaccgt
      181 tcagccccaa cgaggacgcg gtgcacatgt tcgtcaccac gatcgcccgc gcctaccgcg
      241 ggcaacccat ccacaccccc accggcacct ccaactaacg ccctcgctgc ctgtggcggt
      301 ggtggtttgc ctgggtagcc atccaaacgg acgggtagcc aggcggatct gccttgcgag
      361 ggatggattt ggctggttag ccatgcaaac cgccgccggc gaccggtgga tgtccgaaaa
      421 ccgccagtca cggcaaccaa acccgacgga aaaccggccg accggctcag ccggcacgaa
      481 cgtctcccat tcttcgttgc cgacccagct cagccgaggc cgacgcacca ccggaagacc
      541 accgccaccc atcgcgtccg tcttcggcca cagaaaccgg agccgagacc gcacggcccg
      601 gcaagccagc cttcctgggg tttgcgatgt ctccatccca aaccccctcc gcccatcgga
      661 ggtttgcgat gtctacacgc cgcttgggct gtaaacatcg caaaccgccg gcggcgccac
      721 gggagatcac ggcgtcccat cgcgaccgtc tccggccaca gaaaccggaa gcgagcccag
      781 ggtcaggcga gccgggggag gcgctgcttc cactgccgtg cgccgaccgt ggagaagccg
      841 tcctcggtgg cctcccgggc ggtgacctcg atgctcacgt cgaaggcgtc cgcaccgatc
      901 agcacctcca tcgtcgcggt agtagagacc tcgacgtcgg gccaccgaat ggtgaaggtc
      961 gtcgtcgcgg tgctgcgctg ctcgaacgtc gcgtgctcga cgcagaccag gccctcgtag
     1021 tgctccgtcg cggttccgcc gtacgggatc tcgtacgtcg agccgtggtc gaccacgcac
     1081 gacgtcgtac ccgccagaac gtcacgctcg acggaccagg tcagaccggt cgggtcctcc
     1141 ccgcccgtgc ggcccggcgc gagatccggc tcggcgaacg gcgacggccc cgtccacacc
     1201 ggcaggtcca actcgccgcc ggggacggtg atcgtcacag gtcgtggcgg cgcggcggtg
     1261 ttcggccagt ccgatccggc cagtgacagc cgcaaccgtt gacccggatc gaacgcgtag
     1321 gcacaggcct ccagctcgac ctccacgtcg tacacctgac ccggctcgag gggcaccaag
     1381 cggtcgagcc cgtcgcgaca gccgaggttg agcgtgccac gcgacaccag tgccgaggtg
     1441 ccgtcgggga agacgtcaca gaccttcacg gagacgtacg ccgtcggctg gtcgacgctg
     1501 atccgcaacc gcgcgcgcgg atgccccacc aaagtgaccg agtccgcatc ccactcccag
     1561 accaccgacg ccgcgtcgtc gaaccgctgg tccgcgctct gtccccacgg caggtggccg
     1621 gcacagtcga tccaggcgtc gacacccacg tccggcctga cgtcgtgggc cgcgcgtgcc
     1681 gcgaggtcgc gcgacaccac ctccactcga ggcgacggcc actcctcacg tatccagaag
     1741 cctgcggact cgtcgagcac cggcgagggt ctcgtggacg agcgcacgaa gcacgtgacg
     1801 gcaggacgct cggcggagac gccgtcgtcg acgccgttgt cgatcccacg cagccaccgg
     1861 tcccaccacg ccaccatctc cggcatggag tcgatccgcg gaccgggcaa ggccgtgtcg
     1921 gtggacgcgt gtgaccacgg acccgcgagc aaccgatgtg ggacaccctg cgcacgcagc
     1981 cgttcgatcg tgcggaagga gttgttgcgg tagccgtccg cccacccggc gatgatcatg
     2041 gtcggacagg cgatccgctc gtagcccggt cgtaccgacc cgtgccgcca gtacgggcca
     2101 tcgagctgtt cgcggagcca ggtgagcgcc catggctcgc tcgtctcgat ccgccgcacc
     2161 cattcgtccc gccacccgtc tccccagatc gccggcaccg gcggcagcac gttcatcggg
     2221 gtcatgtagt ggcagtagtc gacgaggtcg aggagcttca ggatgccgcc gcggtagtgc
     2281 acgtcgtcgg tgtagcggtc gtccgtgccg tagatcgcga tgatcgcctt caggtgttcg
     2341 gggcgctcgc acgccatctg cagcgagttg aacccggagt acgacgtgcc gtacatcccg
     2401 acgttgccgt cgcaccagtc ctggttcgcc agccagtcga tgacctggtg caggtcctgc
     2461 tgctcccgcg ccgggtactc gtccgtcgcg tcaccaccgg acgatccggt gccgcgcagg
     2521 tcgaggcggc agacggcgta cccgtggtcg tcgcggaacc gcacgtagtc gggtcggtac
     2581 gacgacgtga ggtcgtcctt gcggtacggc agcgcctcga ggatgcacgg ctgcggcccg
     2641 tcgtcgcgct ccggcaggta gagcgtggcc gagagccgga ccccgtcgtc catcacgatc
     2701 aggacctcgc ggcagcgcgg gtcgacgtcc ggagtgctca cgacgtgatc cgcccgtgct
     2761 gctcgagcag cttgacgacg tcgtccacgg gtacgccgtg cgaggtgttg cccgacctcc
     2821 cgaccaccgt cggcgcagcg aagatcgagt tgagcaccgc ctcctcgctc gcctccacca
     2881 ctgctgcgaa gagcggatcg agtccccggc cgatgaccac gggtgtcgac gacgtctgat
     2941 cgtcgcggct gacacgcacg ccggtggacg tggccatgaa gatctccccg ctgccgtgat
     3001 gggccactga acccgtacgg gccaggccga gtccgattcg ccgagccagc ctggcgcagc
     3061 cagctccgtc cacaggggcg tccgtgacga cgagtcctat gcaggaaccc gcgggaggct
     3121 tgcggtcggc gtccgcctca tgctcggacg ccgtgcccag cagccggccg acggggacgc
     3181 cgccgacggt caactggtcg cggacaccga agttcgtcat gagcaggacg ccgacggtgt
     3241 ggccgtcctc ggtcacccgt gacgccgtac cgatgccgcc cttgaagccg aggcagctca
     3301 tgccggttcc cgaccccacc gcgccctcgg ccggcggttc gcccgaaccg accgacgcgt
     3361 cggccgccgc ccaggccgcc ttcacgtcgt ggcgggtcac ctgcatgcgt cgcgcgtcgt
     3421 tgaggaagga gtcgtcgcac tcggccacga ccgggatgat gaagtcctcg gccacgtccg
     3481 ggaaccgttc gagcatcagc tcgcacgcgg cgtcgtacac gcgtccgagt tggagcgtgg
     3541 acgtcaggaa caccggtgtc tccagcgatc cccactcacc ggccgtgatg aatccggtgc
     3601 attccccggc cccgttcagc accgcgccgc ccgccgggag catctcgtgg aacgggtcgt
     3661 cacggagccg tacgaccgtc acgccggtcc gggcgacccc gcgacctttc ggtggatccg
     3721 gctcgtcgcg ccataccgtg gcatgtccga cccctacccc ctcgacatcg aggacggagt
     3781 tcgtcggtcc cgccgggcaa gtcccgatac tgattcccaa gtcagtagcg cgtggcatga
     3841 tctgaatcct gccgtaactg ttcggagagg ggaaccatgc cagccgcgtc ggacgtcccc
     3901 gtcgtctggt cgcagaccac gctcgggcat cgccctgatc gcgaggtgtg gatcggtatg
     3961 ccgatggagg gcagcgagct ccctgagcgc gtgaccgtca tcgagaaggc gttgcgggag
     4021 gccggtcacc cgttccgcga tgcgcaacct catccggtcg aaacgctggg cagcgtccac
     4081 agcgacgagt tcgtccggca cctgtccacc gtctacgagg cgtgggttga cggtggcttc
     4141 gtcagctacg ggcaggaccg ggtcatcccc tacttcttcc cgaccgatgc catgctcggt
     4201 cccatctcgc cgacaccggc cacgtccgtg cacgccgcgg ccgggcggtt ctgttacgac
     4261 acgatgacca cggtcgggcc cggcagctgg gcagcgatca aggctgccgc ggactgtgcg
     4321 ttgacggcgg ccgacctggt gcgttccggg gaggccaggc gcgtctacgc cctgtgccgc
     4381 ccaccgggtc accacgtcac ggccgacgcc tacggcggtt cgtgctacgt caacaacgcc
     4441 gcgttggctg cgcaagcact cgtccaggcg ggtcaccagc aggtggcggt catcgacgtc
     4501 gacgcgcacc acgggaacgg caccgccgcg atcttctatg accgcccgga cgtcacgtac
     4561 gggtcggtcc acgtggatcc agccgccggg tggttccccc atgttgtcgg acacgcagcg
     4621 gagaccgggc gtggcgccgg caccggcacg accctcaaca tcccgctgtc ccctggcagc
     4681 agcgacacgg agttcctcga cggggtgcgt cgactggcgt cgcacgcgat gacccgcggc
     4741 gcaacggcgc tggtcgtctc cctgggcgtc gacgccgcgg tgaacgaccc cgagagcccg
     4801 ctcgaggtca cggaggacgg ctacggacga gccggtgcca tcctgctggg cgagctcgac
     4861 ctccccacgg tcgttgtcca ggaaggtggc taccacctcc cgaccctcgg tccgctggtg
     4921 gcggcgttcc tgtcgcgctg aacgtcggtg gccgggcccg ccgccgcgac gtacggtgaa
     4981 ggacggcgcc gaccgacgcc ctcgcagtca acggtcctgc gaaggagggc ctggtttgtc
     5041 agtacccagc accgaaaacg cctcatcgtt cgacagcacg atcaccctca ccgtcaacgg
     5101 cgtcgtacac aacctcacga tcgacaaccg caccacgctc ctcgacctcc tccgcgagca
     5161 cctcgacctg accgggtcga agaagggctg cgaccacggt cagtgcggag cgtgcacggt
     5221 gctggtcgcg ggacgccgag tcaacgcctg cctgctcctc gcggtcgcgt gcaacggacg
     5281 tcacgtcacg acgatcgaag ggctcgggga cggtgggacg ctgcacccgc tgcaagcggc
     5341 gttcgtgacc catgacggtc tccagtgcgg gtactgcaca cccggccaga tctgc
//
LOCUS       DATBFZ010000304         6692 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_2500076, whole genome shotgun sequence.
ACCESSION   DATBFZ010000304 DATBFZ010000000
VERSION     DATBFZ010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 6692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6692
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2500076"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1472)
                     /gene="nuoL"
                     /locus_tag="VH419_09290"
     CDS             complement(<1..1472)
                     /gene="nuoL"
                     /locus_tag="VH419_09290"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012923843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEY7043848.1"
                     /translation="MSDLLFLIIAIPAASATILLVGGKYTDKWGHLLGALAPLVSFGL
                     GVAAFVDLHGDNEKPLTQHLFTWINAGSFHVEFNLLFDPLSALFVLLITGVGGLIHIY
                     SVGYMANDERRRRFFAYLNLFVAAMLLLVMASDYLALFLGWEGVGLASYLLIGFWQYK
                     DSAAVAAKKAFVVNRVGDLGMAIGIMLMFATFGTMSFAGVEAGMSKVSTATATALGLL
                     LLLGACGKSAQVPLQSWLLDAMEGPTPVSALIHAATMVTAGVYLIVRSNYIYAQTAVA
                     QDFVIAVGLVTLLWGAIIGCAKDDIKKALAGSTMSQIGYMHLAAGLGPAGYALAIFHL
                     LTHGFFKADLFLGSGSVMHAMNDDVNMRRYGALRAILPITFVTFACGYLAIIGVPPFA
                     GFWSKDPIIEAAFGQNFWVGLGSLIGAGITAFYMTRVMLMTFFTKERWEPDVHPHESP
                     KVMTIPLIVLAALSVLGGLMLAGDWIVDFLSPVVGEHTEH"
     gene            complement(1480..1779)
                     /gene="nuoK"
                     /locus_tag="VH419_09295"
     CDS             complement(1480..1779)
                     /gene="nuoK"
                     /locus_tag="VH419_09295"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020391937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEY7043849.1"
                     /translation="MSVTPFLVLSALLFSIGALGVLVRRNAIVVFMCVELMLNACNLA
                     LVSFSRQHGNLDGQVAAFFVMVVAAAEVVVGLAIIMTIYRTRRSASVDDASLLKY"
     gene            complement(1776..2600)
                     /locus_tag="VH419_09300"
     CDS             complement(1776..2600)
                     /locus_tag="VH419_09300"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020391936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEY7043850.1"
                     /translation="MTATFWILAPIMVICALGMVLARKAVHSALMLAVVMVSLAVLYA
                     VQDAPFLFAVQIIVYTGAVLMLFLFVLMLVGVDTADSLVETLRGQRFLAILLGVGFAV
                     LVVAGITQTTIGAVVGLDQANKDGNVPGVATLLFSQYFFAFEVTSALLITAALGAMVL
                     AHRERLSPKPTQRDVVRQRLRDYADRGIHPGALPSPGSFARHNSVDTPALLPDGTPSE
                     LSISRVMRARGTVRDVRADRAIEAAVESDVKQLERTHMGDAPGEPPEHQDEDGGNE"
     gene            complement(2597..3094)
                     /gene="nuoI"
                     /locus_tag="VH419_09305"
     CDS             complement(2597..3094)
                     /gene="nuoI"
                     /locus_tag="VH419_09305"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020391935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HEY7043851.1"
                     /translation="MFKKVVTEQYPFEKKPTAPRFHGRHQLNRWPDGLEKCVGCELCA
                     WACPADAIYVEGADNDDSAGQRYSPGERYGRVYQINYLRCILCGLCIEACPTRALTMT
                     NEYELADNNRADLIYEKQDLLAPLLPGMEEPPHPMRLGDDEGAYYRGVAAAATRSPGE
                     GEVPA"
     gene            complement(3177..4430)
                     /gene="nuoH"
                     /locus_tag="VH419_09310"
     CDS             complement(3177..4430)
                     /gene="nuoH"
                     /locus_tag="VH419_09310"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020578387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEY7043852.1"
                     /translation="MGATVTLAADDLSDFGIDPWWVIVIKVILAFAILLLMTLFIIWL
                     ERRVVARMQHRIGPNVHGPFGLLQSLADGVKLMLKEDLVPRPVDKVVFVVAPLISAIP
                     AFLAVAVIPFGPVVKIPFTDTYTPLQLTDLSVAVLYILAVASVGVYGIVLAGWSSNST
                     YSLLGALRSSAQVISYEVAMGLSLVAVFLYSGSLSTSGIVREQEHLWFCIILLPSFLI
                     YMVSMVGETNRAPFDLPEAEGELVGGFHTEYSSIKFAFFFLAEYINLVTVSMLATTMF
                     LGGWRAPWPLSLWEGANEGYWPVLWFLAKVFIFVYVFIWLRGTLPRLRYDQFMQLGWK
                     VLIPVSLFWILAVGTIRAISLEGEINRTYLFVAAAIAVVIFIGLGFIPEGREEEEAAE
                     VVDDSDARAGGYPVPPMPEQERIRG"
     gene            complement(4430..6328)
                     /locus_tag="VH419_09315"
     CDS             complement(4430..6328)
                     /locus_tag="VH419_09315"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005895.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit G"
                     /protein_id="HEY7043853.1"
                     /translation="MDRVAGEVRLVRLLLVDADLLQRAALQQVGIYEKEPFDSYFSGN
                     TIQICPVGALTSAAYRFRARPFDLISTPSVCEHCASGCALRVDHRRGKVTRRLAGFDP
                     EVNEEWNCDKGRFAFTSTRQQDRLTRPLVRDERGELRPASWPEAFAVAARGLAAAESA
                     VGVLAGGRLTSEDSYAYGKFARAVIGTNDVDFRSRPHSEEEASFLAAHIATRPSPSYA
                     DLEHATAVLLVAFEPEEESPIVFLRLRKGVRHHGTRVYAVSTHTTRGLEKLHGSLVKA
                     TPGAEASVVADLADDGDLALDAGGVILVGERAAASPGLLSAVASLASSSGAKLGWIPR
                     RAGERGALETGLLPNLLPGGRPLADAQARVDLAAAWGLDLVSSTPGRDTSGILSAATD
                     GDIKALVVGAVDIDDLPNHAAAVIALEAVDFLVSLEVRESEVTRRADVVLPVAPMAER
                     SGTLVNWEGRLRPFEQVLDSSALTDVRVLAGIAEEMGKGLGFRAVKGAWDEMTEVGPW
                     DGAWASFEPVAPPAPARPPAGEYVLSTWRTLIDSSRAIDGEPFLAATGKQPVAVVSVA
                     TLDRLGVRAGDPVTVSTDGGSVTLPVTPGDIADDAVWLPTSTGDVQVHRRLRVTAGST
                     VRVERGNA"
     gene            6236..6692
                     /locus_tag="VH419_09320"
                     /pseudo
     CDS             6236..6692
                     /locus_tag="VH419_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002545729.1"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit G"
BASE COUNT         1199 a         2244 c         2271 g          978 t
ORIGIN      
        1 tcgtgctcgg tgtgctcgcc caccaccggg ctgaggaagt cgacgatcca gtcaccggcc
       61 agcatcagcc cgcccagcac cgagagcgcc gcaaggacga tcagcgggat cgtcatcacc
      121 ttcggcgact cgtgcggatg gacgtcgggc tcccagcgct ccttggtgaa gaacgtcatc
      181 agcatcaccc gcgtcatgta gaacgcggtg atgccggcgc cgatcaacga gccgaggccg
      241 acccagaagt tctggccgaa cgccgcctcg atgatcggat ccttggacca gaagccggcg
      301 aacggcggca cgccgatgat cgcgaggtag ccgcaggcga acgtcacgaa ggtgatcggc
      361 aggatcgccc gcagcgcgcc gtagcgccgc atgttgacgt cgtcgttcat cgcgtgcatg
      421 accgatcccg agcccaggaa caggtcggcc ttgaagaacc cgtgggtgag caggtggaag
      481 atcgccaagg catatccggc aggaccgaga ccggccgcga ggtgcatgta gccgatctgg
      541 ctcatcgtcg atccggcgag ggccttcttg atgtcgtcct tcgcgcaacc gatgatcgcg
      601 ccccacaaca atgtgacgag gccgaccgcg atgacgaagt cctgtgctac cgccgtctgc
      661 gcgtagatgt agttggatcg cacgatgagg tagacgcctg ccgtcaccat cgtcgcggcg
      721 tggatcaacg ccgagaccgg cgtcgggccc tccatcgcgt cgagcagcca gctctgcaac
      781 ggcacctgtg ccgacttccc gcaggcgccg aggaggagca acagcccgag cgcggttgcg
      841 gtggcggtcg agaccttgct catcccggcc tccacgcccg cgaaggacat ggtgccgaac
      901 gtcgcgaaca tgagcatgat cccgatggcc atgccgaggt cgccgacccg gttgaccacg
      961 aaggccttct tcgcggccac cgcagccgag tccttgtact gccagaagcc gatcagcagg
     1021 tacgacgcga gaccgacccc ctcccagccg aggaacaacg ccaggtagtc cgacgccatg
     1081 accagcagca gcatggccgc gacgaacagg ttcaggtagg cgaagaaacg ccggcggcgc
     1141 tcgtcgttgg ccatgtagcc gaccgagtag atgtggatga gtccaccgac cccggtgatg
     1201 agcagcacga acagcgccga cagcgggtcg aacaacaggt tgaactccac gtggaagctc
     1261 cccgcgttga tccacgtgaa caggtgttgg gtgagtggct tctcgttgtc gccgtgcagg
     1321 tcgacgaacg cggccacacc gaggccgaag gagaccagcg gggccagcgc gcccagcagg
     1381 tgtccccact tgtcggtgta cttcccgccg acgaggagga tcgtcgcgct cgccgccgga
     1441 atggcgatga tcaggaacag gaggtcagac acggttcccc tagtacttca agaggctggc
     1501 gtcgtcgacc gaggccgagc gtcgcgtccg gtagatggtc atgatgatcg cgaggccgac
     1561 cacgacctca gccgcggcca ccaccatcac gaagaatgcg gcgacctggc cgtcgaggtt
     1621 cccgtgctga cgggagaacg agacgagggc gaggttgcag gcgttcagca tgagctccac
     1681 acacatgaag accacgatcg cgttacggcg caccaacacc cccagggcgc cgatggagaa
     1741 cagcagcgcc gacagaacga ggaaaggcgt gacgctcatt cgttgcctcc gtcctcgtcc
     1801 tggtgttcgg gcggctcgcc aggagcgtcg cccatgtggg tccgctcgag ctgcttgacg
     1861 tccgactcga ccgcagcctc gatcgcccgg tcggcgcgaa cgtcacgcac ggtgccgcgt
     1921 gcccgcatga cccgcgagat cgacagctcc gacggcgtac cgtccggcaa cagcgcgggt
     1981 gtgtcgacgg agttgtgccg ggcgaaggag ccggggctcg gcagcgcgcc ggggtggatc
     2041 ccgcgatcgg cgtagtcgcg caatcgctgc cgaacgacgt ctcgctgcgt cggcttggga
     2101 gacagccgct cgcggtgagc cagcaccatc gcgcccagcg ccgcggtgat gagcagcgcg
     2161 ctggtgacct cgaacgcgaa gaagtactgg ctgaagagca acgtcgccac gcctggcacg
     2221 ttcccgtcct tgttcgcctg gtcgagaccg accacggcgc cgatcgtggt ctgggtgatc
     2281 ccggcgacga cgagcacggc gaagccgacg ccgagcagga tcgccaagaa ccgctggccg
     2341 cggagtgtct cgaccagtga gtcggcggtg tcgacaccga ccagcatcaa gacgaacagg
     2401 aacagcatca agacagcgcc ggtgtagacg atgatctgca cagcgaacag gaacggtgcg
     2461 tcctgcacgg catacagcac cgccaacgac accatcacga cggccagcat caacgccgag
     2521 tgcacggcct tgcgcgcgag caccatgccg agcgcgcaga tgaccatgat cggtgcgagg
     2581 atccagaacg tggcggtcac gccggcacct ctccttcacc gggggaccgg gtcgccgcag
     2641 ccgccacgcc gcggtagtag gcgccttcgt cgtccccgag gcgcatgggg tgcggtggtt
     2701 cctccatgcc tggcagcagc ggcgccaaca ggtcctgctt ctcgtagatc aggtcggcgc
     2761 ggttgttgtc ggcgagctcg tactcgttcg tcatcgtcag cgctctggtc gggcaggcct
     2821 cgatgcacag gccgcacagg atgcagcgca ggtagttgat ctggtacacg cgtccgtagc
     2881 gctcccccgg tgagtagcgc tggccggcgg agtcgtcgtt atcggcacct tcgacataga
     2941 tggcgtcagc ggggcaggcc cacgcgcaca gctcgcaccc gacacacttt tccagaccgt
     3001 cgggccaccg gttcagctgg tgccgtccgt ggaaccgcgg cgccgtcggc ttcttctcga
     3061 acgggtactg ctcggtgacg accttcttga acatcgtgcg gaacgtgacc ccgaagcccg
     3121 cgatcgggtc ccagagctgt tcgcggatgc cgcgtcgacc ggcgtcttcg ggggtgtcaa
     3181 ccacggatcc gctcctgttc gggcatgggt ggtacggggt agccaccggc gcgcgcgtcg
     3241 gagtcgtcga cgacttcggc ggcctcctcc tcttctcggc cctcggggat gaacccgagt
     3301 ccgatgaaga tcacgacggc gatggcggcg gcgacgaaca ggtaggtccg gttgatctcc
     3361 ccctcgaggc tgatcgcccg gatggtcccg actgcaagga tccagaacag cgacacgggt
     3421 atcagcacct tccacccgag ctgcatgaac tggtcgtagc gcaaccgggg cagcgtgccc
     3481 cgcagccaga tgaacacgta cacgaagatg aagaccttgg cgaggaacca caacaccggc
     3541 cagtagccct cgttcgcgcc ctcccagagc gacagcggcc acggtgcccg ccagccaccg
     3601 aggaacatcg tcgtggccag catcgagacc gtgaccaggt tgatgtactc ggcgaggaag
     3661 aagaatgcga acttgatcga cgagtactcc gtgtggaacc caccgaccag ctcgccctcc
     3721 gcctccggca ggtcgaacgg cgcgcggttc gtctcaccga ccatcgagac catgtagatc
     3781 aggaacgacg gcagcaagat gatgcagaac cacaggtgct cctgctcgcg cacgatgccc
     3841 gacgtcgaca gcgacccgga gtagaggaag accgcaacga gcgacagccc catcgcgacc
     3901 tcgtaggaga tcacctgcgc cgacgaccgc agcgccccga gcagcgagta cgtcgagttg
     3961 gacgaccagc ccgccagcac gatgccgtac acgccgaccg aggcgacggc gaggatgtag
     4021 agcacggcga cggacaggtc ggtgagctga agcggcgtgt aggtgtcggt gaacgggatc
     4081 ttcacgaccg gcccgaacgg gatcaccgca acggcgagga aggccgggat cgccgagatc
     4141 agcggcgcaa cgacgaagac gaccttgtcg accggcctcg ggacgaggtc ctccttgagc
     4201 atcagcttca cgccgtcggc caggctctgc agcaggccga acggaccgtg cacgttcggt
     4261 ccgatccggt gctgcatccg cgcgacgacg cgacgctcca gccagatgat gaacagcgtc
     4321 atcaggagca ggatcgcgaa ggcgaggatg accttgatca caatgaccca ccacgggtcg
     4381 atgccgaagt cgctgaggtc gtcggcggcc agcgtcacgg tcgcacccat caggcgttcc
     4441 ccctctcgac gcggaccgtc gatcctgcgg tgacccgcag gcgccggtga acctgtacgt
     4501 cgcctgtgct ggtgggaagc cacacggcgt cgtcggcgat gtctcctggt gtgacaggga
     4561 gtgtgaccga gcccccgtcg gtggaaacgg tgaccgggtc tcccgcgcgc acgccgagcc
     4621 gatcaagtgt cgccacggac accacggcaa caggttgctt tccggtggcg gcgaggaacg
     4681 gctcgccgtc gatcgcacgg ctcgagtcga tgagcgtgcg ccacgtggac agcacgtact
     4741 caccagccgg tggacgtgcg ggggccggcg gcgcgaccgg ctcgaacgac gcccacgcgc
     4801 cgtcccacgg tcccacctcg gtcatctcgt cccacgcgcc cttgacggca cggaacccga
     4861 gacccttccc catctcctcg gcgatgccgg caagcacgcg aacgtcggtc agggccgagc
     4921 tgtccagcac ctgctcgaag gggcgcagcc ggccttccca gttcaccaac gtgccgcttc
     4981 gctcggccat gggcgcgacc ggaagcacga cgtcggcgcg cctcgtcacc tcggactcgc
     5041 ggacctcgag gctgaccagg aagtccaccg cctcgagggc gatcacggca gccgcgtggt
     5101 tgggcaggtc gtcgatgtcg acggcaccca cgacgagagc cttgatgtca ccgtcggtgg
     5161 cggccgacag gatgccgctt gtgtctcggc cgggcgtcga tgacacgagg tcgagtcccc
     5221 aggcggcggc gaggtcgacg cgggcctgtg cgtccgcgag cggacgtccc ccaggcagca
     5281 ggttgggaag caggccggtc tcgagcgccc cgcgctcgcc ggcgcgtcgg ggtatccacc
     5341 cgagcttcgc cccgctcgag gaagcaagcg aggcgactgc gctgagcaga ccgggcgacg
     5401 ctgctgcccg ctcgccgacc aggatcacgc cgccggcgtc gagggccagg tcaccatcgt
     5461 cggcgagatc tgcgaccact gacgcctcgg cgccgggcgt cgccttgacc aggctgccgt
     5521 gcagcttctc cagcccgcgt gtcgtgtgcg tgctcacggc gtacaccctg gtgccgtggt
     5581 gacgcacgcc cttgcgaagc cgcaggaaga cgatgggtga ctcctcctcg ggctcgaagg
     5641 cgacgaggag gacggcggtc gcgtgctcga gatcggcgta cgacggtgaa ggcctcgtcg
     5701 cgatgtgcgc agccaggaac gacgcctcct cctcggagtg cggtcgtgac ctgaagtcga
     5761 catcgttcgt gccgatcacc gcgcgcgcga acttgccgta ggcgtaggag tcctcgctgg
     5821 tcagccgacc gccggccagc acgcccactg cgctctccgc ggcagcgagg cctcgtgctg
     5881 ccaccgcgaa cgcttccggc catgatgcgg gccgaagctc gccgcgctcg tcgcggacca
     5941 gtggacgggt gagccggtcc tgctgccgcg tcgaggtgaa cgcgaacctg cccttgtcgc
     6001 agttccactc ctcgttgacc tcggggtcga atccggcgag ccgacgagtg accttgccgc
     6061 ggcgatggtc gacgcgcaac gcgcacccgg acgcgcagtg ctcgcacacc gacggcgtcg
     6121 agatcaggtc gaacggccgg gcacggaacc ggtacgccgc cgaggtgagg gcgccgacag
     6181 gacagatctg gatcgtgttg ccggagaagt acgagtcgaa cggttccttc tcgtagatcc
     6241 cgacctgctg gagcgcggcg cgttgcagca ggtcggcgtc tacgagaagc agccgtacga
     6301 gtcgtacttc tccggcaaca cgatccagat ctgcccggtg ggcgcgctga ccggggcgtc
     6361 gtaccggttc cgtgcgcggc cgttcgacct gatgtcgacc gactcggtgt gtgagcactg
     6421 cgcgtccggt tgcgcgcagc gcaccgactg gcggcgctcg aaggtgacgc gccggatggc
     6481 cggtgacgac cccgaggtca acgaggagtg gaactgcgac aagggacgct gggcgttcca
     6541 ctacgccgag cagtccgatc ggttgctcgt cccgctggtg cgcgacgcgt ccggccggct
     6601 ggtcgaggcg tcctggccgg ccgcgctcga agcggcggcg aacggtctgc gggcggcgca
     6661 cggcaaggtc ggcgtgctgc ccggcggccg gc
//
LOCUS       DATBFZ010000305         1760 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_538881, whole genome shotgun sequence.
ACCESSION   DATBFZ010000305 DATBFZ010000000
VERSION     DATBFZ010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 1760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1760
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_538881"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(343..1686)
                     /locus_tag="VH419_09325"
     CDS             complement(343..1686)
                     /locus_tag="VH419_09325"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY7043854.1"
                     /translation="MNRTLRAQIALATALVALVVVALAGALIALRIDHRDRTIVDAQL
                     AARAERVTADIDKLLIDGNSNQPGDDGYGDLLDGSESLVRLFDGATLVAQRGAIPDQP
                     LMPPAGSGLSTITVDGAPWRSYVVSSRDGIRLQVLQSLQPVNDRRTANTRLVILVTLL
                     AALVSGAAGWLVGSRVLRPLHRLTSGAVAIANDPDPNHRLPAVTGPAEVAALSTTLNG
                     MLDRLGASTETTRQFAADVGHELRGPLTASTTYLETLLTSVELTADARQAVAAAHEQQ
                     QRMVATLGALQVLARGDARALPPPTDLEPGLLVEELVRLARRRHPQITFTLNDSTDGA
                     TVSGWSDGLRIAIDNLLNNAAVHGRPAGTVDVSVHANDGHVAVCVEDDGPGIDPNDLD
                     LVRKRFMRRAAATTPGSGLGLALVDQQALLHGGSLTLANSKRGGLRAELRLASTP"
BASE COUNT          251 a          514 c          693 g          302 t
ORIGIN      
        1 gtgccgttgg gacggaggat cgtgggtcag ccatccacca gacggcaagc tggcggtagc
       61 gcgacggtcg gctgatgcgg tggcctggga ggtcgtcatc acggggctcc ttcgcctcgg
      121 cggcgaccgc gatcgtcccg ccgccacctg aagagggctt aagtgccagc tgacaaggtc
      181 cttggaggtg tcctgctcat tcgtggcgca tggcgggagc cgggtcgagc ccggcgatca
      241 tgccgaccac cgaggagacg acaggggcaa ggccggccgc tgagcaggag agatggtccg
      301 ctggtcgctg gtcacccgcc tgagggacgc ctgtgaccgg cgtcagggtg tcgatgccag
      361 gcgcaattct gcccgaagac ctcctcgctt tgagttggcg agggtgaggc tgccgccgtg
      421 cagcaaggct tgctggtcaa ccagcgccaa gccgagaccg ctccctggtg tggttgcggc
      481 cgcccggcgc atgaaccgtt tccggacaag gtcgaggtcg ttggggtcga tgcctggtcc
      541 atcgtcctcg acgcagaccg cgacgtgtcc atcgttggcg tgcacgctca cgtcaacggt
      601 gccagcgggt cggccgtgga cggccgcgtt gttgagaaga ttgtcgatgg cgatcctcag
      661 accatcgctc cagcccgaga ccgtggctcc gtcggtggag tcgttcaatg tgaaggtgat
      721 ctgtgggtgc cggcggcgcg cgagtcggac gagctcctcg accaggaggc cgggctcgag
      781 gtcggtcggg ggtggtagcg ctcgggcgtc tccccgcgcg agcacttgga gcgcgcccag
      841 ggtcgcgacc atgcgctgtt gttgctcgtg cgcggcggcg accgcttggc gggcatcggc
      901 tgtcaactcg actgaggtta gcagcgtctc caggtaggtg gtggaggcgg tgaggggtcc
      961 gcggagctcg tggccgacgt cggccgcgaa ctggcgggta gtttcggtgc tggcaccgag
     1021 tcggtcgagc ataccgttca gggtggtcga gagtgcggcg acctcggccg ggccggtgac
     1081 ggcagggagc cggtggtttg ggtcggggtc gttcgcgatg gcgacggcgc cgcttgtcag
     1141 ccggtgcagg gggcggagca ctcggcttcc gacgagccag cccgcggccc cagacacgag
     1201 ggcggcgagg agggtcacga ggatcacgag gcgggtgttc gccgttcggc ggtcgttgac
     1261 gggttggagg gactggagca cttggagcct gatgccgtcg cgcgaagaca cgacatatga
     1321 ccgccacggc gcaccgtcaa cggtgatggt gctcaggccg ctgccggctg gtggcatcag
     1381 gggttggtcg gggatcgcgc cccgttgggc aaccagggtg gcgccgtcga acaggcgcac
     1441 cagtgactca ctgccgtcga gcaggtcgcc gtatccgtcg tcgccgggct ggttgctgtt
     1501 gccgtcgatg agcaacttgt cgatgtcggc ggtgacgcgt tcggctcggg cggcgagctg
     1561 cgcatcgacg atggtgcggt cacggtggtc gatgcgaagt gcgatgagcg ctccggcgag
     1621 cgccacgacg accagtgcga cgagtgcggt ggcgagcgcg atctgtgccc gcaaggttcg
     1681 gttcacgctt cacctgccgg ggcgcaggac gaatccggtg ccgcgtacag tatggaggac
     1741 acggggggtg tggtcggctt
//
LOCUS       DATBFZ010000306         3584 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_866284, whole genome shotgun sequence.
ACCESSION   DATBFZ010000306 DATBFZ010000000
VERSION     DATBFZ010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 3584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3584
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_866284"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1378
                     /locus_tag="VH419_09330"
     CDS             <1..1378
                     /locus_tag="VH419_09330"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEY7043855.1"
                     /translation="SELEDALREIGFTSFVQPTSGEVVDVDGLQVMIQSLTSPTDGPI
                     GDSSLWLSDGVQTVLNQNDARPAELDAVKALGPVDGYLVQFSGAIWFPMVYELPARAK
                     QAMGLTKRERQFDRTLRYIDELGARVVFPTAGPPCFLDEELWGFNDIFGDESNIFPDQ
                     TVYLDWLAEKGHHEGRLLLPGTVAALDEPDCPLTHPYDPATIYASNETKTAYLKDMQA
                     RRMPEVEALRETWAHPEIDILAALREWFAPLLVEAEHIASGIDGGVRFTCEDDTRGDV
                     DLLIDFVACQVRAYAGEKVRYRFRTRRAYVEALIAEHEIDWVNSLFLSCRFSAHRIGP
                     YNEFVYVFFKCLSEERLNYAEGWFAEQNAAEAAETITLDGWDLQRRCPHLKADLSRFG
                     SIDDGVLTCQMHGWRWRLSDGKCLTSVGHELRCAPAGTPVPGEPIPPVEHHDHRTQHD
                     DEAVYGET"
     gene            complement(1492..2379)
                     /locus_tag="VH419_09335"
     CDS             complement(1492..2379)
                     /locus_tag="VH419_09335"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEY7043856.1"
                     /translation="MTGRTTLQLDTDTVVLGDCAHVLPTLPDSAFQLIYVDPPFNTGK
                     AQQRRTLATVRDESGDRTGFAGRRYATAELARHSYLDVHDDYLGMLEPLLHQARRLLT
                     TSGTLYLHIDYREAHYVKVLLDRIFGRDAFLNELIWAYDFGGRARDRWPAKHDTILVY
                     VRTPGGHFFDQDAVDRIPYLAPGLVGPEKAARGKLPTDVWWHTIVATNGREKTGYPNQ
                     KPMGIVRRIVQASSRPGDRVLDFFAGSGTTGAVAAELDRRFLLVDDNPAAIEVMRARL
                     PDSTRFVDPTAPTAAPTTS"
     gene            3089..3161
                     /locus_tag="VH419_09340"
     tRNA            3089..3161
                     /locus_tag="VH419_09340"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3122..3124,aa:Leu,seq:tag)
     gene            complement(3169..>3584)
                     /locus_tag="VH419_09345"
     CDS             complement(3169..>3584)
                     /locus_tag="VH419_09345"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020387178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEY7043857.1"
                     /translation="GKDVSLSDYRGRKVIVYFYPAAMTPGCTTQACDFRDSLDSLAAA
                     GYAVLGISPDAPDKLAKFRERDAVTFPLLSDATKSTLQAYDAYGEKTMYGKKVTGVLR
                     STFIVDPDGRIEDAQYNVKAKGHVAKLRRDLGVDD"
BASE COUNT          583 a         1153 c         1263 g          585 t
ORIGIN      
        1 gtccgaactc gaggacgcgt tacgggagat cgggttcacc tccttcgtgc aaccgacgtc
       61 cggcgaggtg gtcgatgtcg acggactgca ggtgatgatc cagtcgctga cctcgccgac
      121 cgacggaccg atcggcgact catcgctatg gctgtccgac ggcgtccaga ccgtgctgaa
      181 ccagaacgac gcccggcccg ccgaactcga cgcggtcaag gcactcggcc cggtcgacgg
      241 ctacctggtg cagttctccg gcgcgatctg gttcccgatg gtgtacgagc tgccggcccg
      301 cgcgaaacag gcgatggggc tgaccaagcg cgagcggcag ttcgaccgga cgctgcgcta
      361 catcgacgaa ctcggcgccc gggtcgtgtt cccgaccgcc gggccgccgt gcttcctcga
      421 cgaggagctg tgggggttca acgacatctt cggcgacgag tcgaacatct tcccagacca
      481 gacggtgtat ctggactggc tggccgagaa ggggcatcac gagggccggc tgctgctgcc
      541 gggcacggtt gccgcgctcg acgagcccga ctgcccgctc acgcacccgt acgacccggc
      601 gacgatctat gcgtcgaacg agacgaaaac cgcgtatttg aaagacatgc aggcgcggcg
      661 gatgcccgag gtcgaggcgc tgagggagac ctgggcacac ccggagatcg acatcctggc
      721 ggcgctgcgg gagtggttcg cgccgctgct ggtcgaggcc gagcacatcg cctccggcat
      781 cgacggcggc gtccggttca cctgcgagga cgacacccgg ggcgacgtcg acctcttgat
      841 cgacttcgtc gcctgtcagg tccgggcgta cgcaggggag aaggtgcggt accggttccg
      901 tacccgacgg gcgtacgtcg aggcgctgat cgccgagcac gagatcgact gggtgaactc
      961 gctgttcttg tcctgccggt tctccgccca ccgcatcggc ccgtacaacg agttcgtcta
     1021 cgtcttcttc aagtgcctgt ccgaggaacg gctgaactac gccgagggct ggttcgccga
     1081 gcagaacgcc gccgaggccg cggagacgat cacgctcgac ggctgggacc tgcagcgtcg
     1141 gtgcccgcat ctgaaggcgg acctgtcccg gttcgggtcg atcgacgacg gcgtcctcac
     1201 ctgccagatg cacggctgga gatggcggct gtctgacggc aagtgcctga cgtcggtcgg
     1261 ccacgagctg cgctgcgcgc cggccgggac gccggtgccc ggcgagccga tcccgccggt
     1321 cgagcaccac gaccaccgca ctcaacacga cgacgaggcg gtctacggcg agacatgatt
     1381 cccgccgacc tcgctcgccc atcgcccgga cgcccggcgg gacacgttga gtggcgcgta
     1441 cacggttgag tggcacggta tacaacgtca ctcaacgcag gaacgcgcca ctcaactggt
     1501 ggtgggggcg gcagtgggcg cggtcgggtc gacgaatcgg gtggagtcgg ggaggcgggc
     1561 gcgcatgacc tcgatcgctg cgggattgtc gtcgacgagg agaaaccgcc gatcgagttc
     1621 ggcggcgacg gctcccgtgg tgccgctgcc ggcgaagaag tcgagcaccc ggtcgcccgg
     1681 gcgcgacgac gcctgcacga tccggcgcac gatgcccatc ggtttctggt tcggataccc
     1741 ggtcttctcc cggccgttgg tggccacgat ggtgtgccac cagacgtcgg tgggcagctt
     1801 gccccgtgcg gccttctccg gaccgaccaa cccgggcgcg aggtagggga tccggtcgac
     1861 ggcgtcctgg tcgaagaagt gcccgcccgg cgtgcggacg tagaccagga tggtgtcgtg
     1921 cttggccggc cagcggtcgc gggcccggcc accgaagtcg taggcccaga tcagctcgtt
     1981 caggaatgcg tcgcggccga agatgcggtc gagcaggacc ttgacgtagt gcgcctcgcg
     2041 gtagtcgatg tgcaggtaga gcgtcccgct cgtggtcagc agccggcgcg cctggtgcag
     2101 cagcggctcg agcatgccga gatagtcgtc gtgcacgtcg aggtagctgt gccgcgcgag
     2161 ttcggcggtg gcgtaccggc gccccgcgaa cccggtccgg tcaccgctct cgtcgcggac
     2221 ggtggccagg gtccgccgct gctgcgcctt gccggtattg aacggcgggt cgacgtagat
     2281 gagctggaat gctgagtcgg ggagcgtcgg caggacgtgc gcacagtcgc cgagcacgac
     2341 agtgtcggtg tcgagctgca gcgtcgtccg gccagtcatg gggcgcactg taggacaccg
     2401 gtattgtcag aggtaattgc tacgttcgtt cgatggcgcg agtaccacgc tggaccgacg
     2461 aggatctacg agctgcggtg gccgcgagcc ggtcgcgcgc cgaggtctgc aggcgactgg
     2521 gacttcgccc aggcggcggt acctatgcct cgttaggtcg ccatattcag cgtcttggca
     2581 tcaacgacag ccacctgccg aagatggtcg acggccgcgt ccgcccgccg cgctcttgga
     2641 cggacgacga cctacgagag gcggtccgcg aaagcgtgtc cttggccggc gtgcagcgcc
     2701 gactcggata cagcccgagc ggcggaatgc accggttcct ctcgggctac atccagaagc
     2761 tgaagttgga cacgaatcac ttcactggac aggcgtgggc cgcaggcaga tcccctgtcg
     2821 gtgccttcaa ggcccggccc ctggacgaga tcctcgtgcg tgactccacc tatgtgagca
     2881 gtgcccgcct acgccgacgg ctagtccgag catgggttga agtcggcgct gtgcgagcga
     2941 tgcggaatcg atagctggca gaacctgccg ctgccgctcg cactggatca cgtgaacggc
     3001 gatccgcggg acaatcgact cgagaacctc cgtatcctgt gcccgaattg ccatgcactc
     3061 acggatacgt ggtgcgggcg gaacaggggc cggcgtactc caaaggcaga gagaacaggc
     3121 ttagaccctg tccagtgcgg gttcgaatcc cgccgccggc actgatcgtc agtcgtcgac
     3181 gccgaggtcg cggcgcagtt tcgcgacgtg gcccttggcc ttgacgttgt actgggcgtc
     3241 ctcgatccgg ccgtccgggt cgacgatgaa cgtggaccgg aggacgccgg tgaccttctt
     3301 gccgtacatc gtcttctcgc cgtaggcgtc gtaggcctgc agcgtcgact tcgtcgcgtc
     3361 cgagagcagc gggaacgtga ccgcgtctcg ctcacggaac ttcgcgagtt tgtccggcgc
     3421 gtcgggcgag atgccgagca ccgcgtagcc ggcggcggcg agggagtcca ggctgtcgcg
     3481 gaagtcgcac gcctgggtgg tgcaacccgg cgtcatcgcc gccggataga agtagacgat
     3541 caccttccgg ccgcgatagt cggacagcga gacgtccttg ccgt
//
LOCUS       DATBFZ010000307         7117 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_5684323, whole genome shotgun sequence.
ACCESSION   DATBFZ010000307 DATBFZ010000000
VERSION     DATBFZ010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 7117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7117
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5684323"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(158..856)
                     /locus_tag="VH419_09350"
     CDS             complement(158..856)
                     /locus_tag="VH419_09350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043858.1"
                     /translation="MGNTARVLGGLSVLAAPLAIGIGDQFRMAAENNNDVAVTAGDDL
                     LAETTRQLASIDAHLGLFQTAAWFFYAAALLTIPALITIWRLSVAGSRKWAWAGATMA
                     AIGVIGQTVHLTGYFALMQESSQSLDLESAAKLQIALDQNVFLGALFFVPFLLCALGC
                     TLPQAIGLRRAGVIPMWACLALVAATLVMFVVGSVPWTSALWTVLMVAGFAPAALAAM
                     RTPQPVRDEVLVTV"
     gene            1083..2936
                     /locus_tag="VH419_09355"
     CDS             1083..2936
                     /locus_tag="VH419_09355"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEY7043859.1"
                     /translation="MARPRPTVSELTVAAFCGIWAAVALAPEGWTVVLAPAFALVVLL
                     SRRRPGLAGFAFAVVSILAAVLGVPADSPAALPAGFAILFAAGRYADRRVGLAVLAAN
                     VGAVTAVDEFSVENLLFGTFLLGAVYVFGVLVRRRTVGARMAADDLSTLVALDPASLA
                     AAAVAEERTRLAGEALAVVRRAVDQMCRQANRAESDLDTSALEAVQAGGREAVTELRV
                     LLGLLRTDEPVEDAPPARPRRWPGVVVTVVALAGMFLLEDAATLDDVAWQGVALSIAV
                     MAGAAIGRMDVVLGCLVASAAVLVSVSADVPLVYGAWDTAACALLVWTAATDGRVRAY
                     VAAGCLATLTVLDVHRHVPGNEAFLVAVFAVSATAGHLWAERERAERAAIETAAGLRS
                     QQSAIAAAAVRAERLRLARDLHDVVSHAIGVMVLQAGAAQALRLTDPAAAREAVRSVQ
                     NAGIAATSELVVLFGLVDSGALGAAGVAEPEEADLDALADRMRAGGLTVDLDSAGPPE
                     DPAVRATAYRVVQEALTNAARHAPGAAVSIVVRPERGWLRVEVRNSGAVGVDTATPEP
                     PARPTNGSGFGLVGLAERVRALGGDLAAGPADDGGFVVSARVPLVAAAVSP"
     gene            2933..3604
                     /locus_tag="VH419_09360"
     CDS             2933..3604
                     /locus_tag="VH419_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY7043860.1"
                     /translation="MSDPIRVVIADDQDLVRGGLRAILTAVPDIAVVGDAADGATAAQ
                     VAVDENADVVLMDVQMPGTDGLEGTRLVVESGTGCRVLMLTTFDLDEYVVEALRRGAS
                     GFLLKTTPPDELAAAIRACHAGDLLFAPTVTRRLVETYVRAAPQSAELDRSVKSLTDR
                     ELDVLRELARGRSNAEIAGALYLGEATVKTHVTRILGKLGLRDRVQAVVLAYEAGVVR
                     PGDDR"
     gene            3786..4013
                     /locus_tag="VH419_09365"
     CDS             3786..4013
                     /locus_tag="VH419_09365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043861.1"
                     /translation="MATNVIQFEDSLTPCGQRKGGERAVTEDQQGLIHEQTTYACGCT
                     SSKTEYHDGSVHMSMVHHKGKVLLDQVLSGE"
     gene            4006..5760
                     /gene="frdA"
                     /locus_tag="VH419_09370"
     CDS             4006..5760
                     /gene="frdA"
                     /locus_tag="VH419_09370"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020385768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HEY7043862.1"
                     /translation="MSEGEFAATHDVLLVGGGGAGLRAAIAIAETNPHLDVAVMSKVY
                     PMRSHTVSAEGGAAGVIAEGDTLEEHIYDTISGSDWLGDQDAIEAFVEEAPKELLQLE
                     HWGCPWSRQPDGRIAVRAFGGMKKMRTWFAADKTGFHMLHALFQTSLKYDALTRYDEG
                     FVTKLLVDDGKVHGVVAIELFTGKIYSIAAKAVILCTGGCGKVFPFTTNANINTGDGM
                     ALAYRAGAPLKDMEFVQYHPTGLPFTGILITEAARAEGGWLLNRDGYRYLQDYDLGTP
                     SPTPVLRSMELGPRDRVSQAFVHEVEKGRTTETPYGQVVHLDLRHLGAKKIEEKIPFV
                     RELCLKYERIDPVKELIPVRPVVHYMMGGVHTDMHGATPIQGLYAAGETACVSINGAN
                     RLGSNSLPELLVFGSRAGKAAAEYAAGADGPPASVTSQAADEERRLERDLLGKDAGSE
                     RVSAIRDEMQATMEDSAGIYRTGPSMAKGLDTLAELQQRMRNVGVADTSRPFNTELTA
                     ALELANMLDIAECMLQSGLQREESRGAHQRTDFPKRDDERFLTHLLVSREPDGTSRVE
                     RLPVTITRWPPGERVYGR"
     gene            5763..6506
                     /locus_tag="VH419_09375"
     CDS             5763..6506
                     /locus_tag="VH419_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020385769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HEY7043863.1"
                     /translation="MADGIGLRVSRYAPATDSGPHWQDYDVPLREDWTVLDALNHIKN
                     EIDPTLSFRWSCRMGICGSCGMNVDGAPRLTCGTFLVDFAPGPIRVEPLSNFPVIRDL
                     VIDMSDFMRKLPTVKPWIIRDEELPVEEGEYLQTPEELDAYKQYSMCINCMLCYAACP
                     VYGLDPNFVGPAAIALAERYDLDSRDQGSRERIDVLIDHEGVYGCTFVGECTRVCPKH
                     VDPAGAIQRYKLKAAQESVKALLLPRGSR"
     gene            6503..6892
                     /locus_tag="VH419_09380"
     CDS             6503..6892
                     /locus_tag="VH419_09380"
                     /inference="COORDINATES: protein motif:HMM:NF014366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit C"
                     /protein_id="HEY7043864.1"
                     /translation="MSELRTYRWQVKTFWWVKKWTYFLFVMRELSSIFVAWFVLYLLI
                     LIAAVNRGDAAYEDFKDFASNPAIVVLNLVAFAFVVLHVITWFSLTPKAMDVRLGDRP
                     VPSWAVVASQYAALVVVSAVILWLVTG"
     gene            6889..>7117
                     /locus_tag="VH419_09385"
     CDS             6889..>7117
                     /locus_tag="VH419_09385"
                     /inference="COORDINATES: protein motif:HMM:NF014378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit D"
                     /protein_id="HEY7043865.1"
                     /translation="MRTRVEPFVWLLFSGGGVLAAVFLPMLAFLFGLAYPLGLLDWPE
                     RDHLNAVVSNPLTILVLLGLFVMLLVHSAHRF"
BASE COUNT         1104 a         2415 c         2469 g         1129 t
ORIGIN      
        1 accgacagtt ctcgcgggcg ggcgcgcggt tgcgtccgtg aaggcggccg cagaggccgc
       61 cgcttctacg gacgcaacca gctggcggcg cggttgcgtc cgtggcggta gctgccggag
      121 cagccgccgc cacggacgca agcaggcgcc aggtcgctca gacggtgacc aggacctcgt
      181 cgcgtactgg ctgaggggtg cgcatcgcgg ccagcgccgc cggcgcgaag ccggccacca
      241 tcaagaccgt ccagagcgcc gaggtccagg gcaccgaacc gacgacgaac atcacgaggg
      301 ttgccgcaac caacgcgagg catgcccaca tcgggatcac accggcgcgg cgcagtccga
      361 tcgcctgtgg gagtgtgcag cccaacgcgc acagcaggaa cgggacgaag aacagggcac
      421 ccaggaagac gttctggtcg agtgcgatct ggagcttggc cgctgactcc aggtcgagtg
      481 actgcgagct ctcctgcatg agagcgaagt agccagtcag gtgcaccgtc tggccgatca
      541 cgccgatggc ggccatcgtc gctcccgccc atgcccattt ccgcgaaccg gctaccgaca
      601 gccgccagat cgtgatcaac gccgggatgg tcagcagcgc cgccgcgtag aagaaccagg
      661 cagccgtctg gaagagcccg aggtgcgcgt cgatgcttgc gagctgccgc gtcgtctcgg
      721 cgagcaggtc gtcgccagcc gtcaccgcca cgtcgttgtt gttctccgca gccatccgga
      781 actggtctcc gatcccgatt gcgagcgggg ccgccagcac ggacaaccca cccagtactc
      841 gtgccgtgtt tcccatggtc gttctccgtc tcctccgggc gttgcgagcc cgtctctctc
      901 ggtatgccgg aaggccggca gtcggacatc gcaaaccctg ccgcgcgaca accgcttctc
      961 gcgtcggtcg agacgcatcg actaggtacg acggtcgacc gctcggggca tcgcgggatt
     1021 gatgacggcg agtagatctc aagatggatg tccgcccacg actgctcagg tacggtcacg
     1081 ccatggcacg acctcggccg acggtgagcg agctgacagt ggccgcgttc tgcggcatct
     1141 gggcagcggt cgctctggcc cccgagggct ggacagtggt actcgcgccg gcgttcgcgt
     1201 tggtcgtact gctgtcacgg aggcggcccg gtctcgccgg gttcgcgttc gctgtcgtgt
     1261 cgatcctggc cgcagtcctc ggagttcctg cagacagccc tgccgcgttg cccgccggct
     1321 tcgccatcct ctttgccgcc ggccggtacg ccgatcgccg cgtcgggctg gcggtcttgg
     1381 cggcgaacgt cggggcggtc acagccgtcg acgagttctc ggtggagaac ctgctcttcg
     1441 ggacgttcct gctcggtgcc gtgtacgtgt tcggcgtact ggtgcgacga cggaccgtgg
     1501 gcgcacggat ggctgctgac gacctgtcga cgctcgtcgc gctggatccc gcctccctcg
     1561 ccgccgcagc ggtggccgag gagaggaccc ggctggcggg agaggcattg gccgtcgtac
     1621 gccgagcggt cgaccagatg tgtcgccagg ccaaccgcgc cgagtccgat ctcgacacct
     1681 cggcactgga ggctgttcag gccggcggtc gggaggcggt caccgagctg cgcgtgttgc
     1741 tggggctgct ccggaccgac gagccggtgg aggacgcgcc accagcccgg ccgcggcgtt
     1801 ggcctggcgt ggtggtgacc gtggtcgcac tcgccgggat gttcctgctg gaggatgcag
     1861 cgaccctgga cgatgtcgcc tggcagggcg ttgccttgtc gatcgcggtg atggccgggg
     1921 cggcgatcgg acgcatggat gtcgtcctcg gatgtctcgt cgcgtcggcg gccgtactcg
     1981 tctccgtgtc cgccgacgta ccgctggtct acggtgcgtg ggacaccgcc gcctgcgcac
     2041 tgctcgtctg gacggcggcg accgacggtc gagtgcgagc gtacgtcgct gcgggttgtc
     2101 tggcgacgct gacggtcctg gacgttcacc gacacgtgcc gggcaacgag gcgttcctcg
     2161 tcgctgtctt cgcggtgtcg gcgacggccg gtcacctgtg ggcagagcgc gagcgagcag
     2221 aacgcgccgc gatcgagact gcggccgggc tgcgttcgca gcagagcgcc atcgccgcag
     2281 cagcagtgcg ggccgagcgg ctgcggctgg cgcgcgacct ccacgacgtc gtcagccacg
     2341 cgatcggggt catggtgctg caagccgggg ccgctcaggc gcttcggctc acggacccgg
     2401 cagcagcccg ggaggcggtc cgctccgttc agaacgccgg gatcgcggcg acgtcggagc
     2461 tcgtggtgct cttcggcctc gtcgactccg gggcgctggg cgcggccggt gttgccgagc
     2521 ccgaggaagc ggatctcgac gcgttggcgg accggatgcg tgccggtggt ctgacggtcg
     2581 acctggattc cgcggggccg ccggaggacc cggcggtacg ggcgacggcg tatcgcgtcg
     2641 tgcaggaggc gctgaccaac gcggcccggc acgcccctgg cgcggccgtg tcgatcgtgg
     2701 ttcgcccgga gcgtggctgg ctgcgggttg aggtgcgcaa cagcggtgcg gtcggcgttg
     2761 acaccgcgac gcccgaaccg cctgctcggc cgacgaacgg cagcgggttc ggcctggtcg
     2821 ggctcgccga gcgtgtccgg gcgcttggcg gcgacctcgc tgctgggcct gctgacgatg
     2881 gaggcttcgt tgtcagcgca cgggtgccgc tcgtggccgc ggcggtttcc ccgtgagcga
     2941 cccgatccgc gtcgtcatcg ccgacgacca ggacctcgtc cgcggtgggc tgcgcgccat
     3001 cctgaccgct gttcccgaca tcgcggtcgt gggagacgca gccgacggag cgacggcagc
     3061 gcaggtcgcc gtcgacgaga acgccgacgt cgtcctcatg gacgtccaga tgcccggcac
     3121 cgacgggctg gagggcaccc gactcgtcgt ggagtccggg accggctgcc gggtgctcat
     3181 gctgaccacg ttcgatctcg acgagtacgt cgtcgaggcg ctgcgccgtg gcgcaagtgg
     3241 ctttctcctg aagacgaccc cgccggacga gctcgccgcg gcgatccggg cgtgtcatgc
     3301 cggcgacctg ctcttcgcgc cgacggtgac gcggcgcctg gtcgagacgt acgtgcgagc
     3361 cgcgccgcag tcagccgagt tggatcgcag tgtcaagtcg ctcaccgatc gcgagctcga
     3421 cgtcctgcgt gagctggcgc gcggccgctc caatgccgag atcgccggcg cgctctacct
     3481 gggtgaggcc acggtcaaga cgcacgtgac ccgcatcctc gggaaactcg gactgcgcga
     3541 tcgagtccag gcggtggtgc tggcgtacga agccggcgtc gtacgccccg gcgacgaccg
     3601 ctgacccccg ggcgtacgtc cgtgcagcgg gttgccgggg cagccgctgc tacggacgca
     3661 agcagcggag gcggggacct tcacaaaccg ggcgcgacgg agcaggctgg ggtgggcgtg
     3721 tcggccgacg agcccgcgcg atgagtccct gtactccggc cctgtactcc gggaggtgcc
     3781 agccagtggc caccaacgtc atccagttcg aggattcgct caccccctgc gggcaacgga
     3841 agggcggtga acgtgcggtg accgaagacc agcagggact gatccacgag cagacgacct
     3901 acgcctgcgg ctgcacgagc tcgaagacgg agtaccacga cggcagcgtg cacatgtcga
     3961 tggtccacca caagggcaaa gtgctgctcg accaggtgct cagcggtgag tgaaggggag
     4021 ttcgccgcca cacatgacgt actcctcgta ggcggcggtg gcgccggact ccgcgccgcc
     4081 atcgccatcg ccgagaccaa cccgcacctc gacgttgccg tcatgtcgaa ggtctacccc
     4141 atgcgcagcc acaccgtctc cgcggaaggc ggcgcggccg gggtgatcgc cgagggcgac
     4201 accctcgagg aacacatcta cgacacgatc tccggcagcg actggctcgg tgaccaggac
     4261 gccatcgagg cgttcgtcga ggaggcgccg aaagaactgc tgcagctcga gcactggggt
     4321 tgcccgtgga gccggcagcc ggacggtcgc atcgccgtcc gggcgttcgg cgggatgaag
     4381 aagatgcgca cgtggttcgc cgccgacaag accggcttcc acatgctgca cgcgctcttc
     4441 cagacctcgc tgaagtacga cgcactgacc cgctacgacg aaggcttcgt gaccaagctg
     4501 ctcgtcgacg acggcaaggt gcacggcgtg gtcgcgatcg agctgttcac cggcaagatc
     4561 tactcgatcg ctgccaaggc cgtaatcctg tgcaccggcg gctgcggcaa ggtcttcccg
     4621 ttcaccacca atgccaacat caacaccggc gacggcatgg ccttggccta ccgtgcggga
     4681 gcgccgctga aggacatgga gttcgtccag taccacccga ccggcctgcc gttcaccggc
     4741 atcctcatca ccgaggcggc ccgggccgag ggcggctggc tgctgaacag ggacggctac
     4801 cgctatctcc aggactacga cctcggcacc ccatcgccga caccggtcct gcgcagcatg
     4861 gagctcggtc cacgcgaccg ggtgtcgcag gcgttcgtcc acgaggtcga gaagggccgc
     4921 acgacggaga cgccgtacgg ccaggtcgtc cacctcgacc tgcgccacct cggcgcgaag
     4981 aagatcgaag agaagatccc cttcgtgcgg gagctctgcc tgaagtacga acggatcgac
     5041 ccggtgaaag agcttatccc ggtccgtccc gtcgtccact acatgatggg tggcgtccac
     5101 accgacatgc acggcgcgac tccgatccaa ggcctgtacg ccgccgggga gacagcgtgc
     5161 gtcagcatca acggtgcgaa ccggctcggc tcgaactcgc tgcccgagct cctcgtcttc
     5221 ggctcccgcg ctggaaaggc tgccgcggag tacgccgccg gggccgacgg cccacccgcc
     5281 tccgtcacga gtcaggccgc ggacgaggag cgccggctcg agcgcgacct cctcggcaag
     5341 gacgccggca gcgaacgggt ctcggccatc cgggacgaga tgcaggcgac gatggaggac
     5401 agtgccggga tctaccgcac cggaccctcg atggcgaagg gactggacac gctcgccgag
     5461 ctgcaacagc ggatgcgcaa cgtcggcgtc gcagacacca gccggccgtt caacaccgag
     5521 ctgacggcgg cgctcgagct cgccaacatg ctcgacatcg ccgagtgcat gctgcagtcg
     5581 ggcctgcagc gagaggagtc tcgcggggct caccagcgaa ccgacttccc caagcgcgac
     5641 gacgagcgct tcctcactca cctactggtc agccgcgaac cggacgggac ctcgcgggtc
     5701 gagcggctgc cggtgacgat cacccgatgg ccaccgggcg agcgggtcta cgggaggtag
     5761 tcatggcaga tggcatcggc ctgcgggtat cgcggtacgc gccggcgacc gactcaggac
     5821 ctcactggca ggactacgac gtcccccttc gcgaggactg gacggtcctc gacgcgctca
     5881 accacatcaa gaacgagatc gacccgacgt tgtcgttccg ctggtcgtgt cggatgggca
     5941 tctgcggcag ctgcggcatg aacgtcgacg gtgccccacg cctcacgtgc ggcacgttcc
     6001 tcgtggactt cgcgcccggc ccgatccgtg tcgagccgct ctccaacttc cccgtcatcc
     6061 gtgacctcgt catcgacatg agcgacttca tgcggaagct gccgaccgtc aaaccgtgga
     6121 tcatccgcga cgaggagctg cccgtggaag agggcgagta cctccagacg ccggaggagc
     6181 tcgacgcgta caagcagtac agcatgtgca tcaactgcat gctctgctac gccgcctgcc
     6241 cggtctacgg tctcgacccg aacttcgtcg gtccggccgc gatcgcgctg gccgaacgtt
     6301 acgacctcga ttcccgggac caggggagcc gtgagcggat cgacgtcctc atcgaccacg
     6361 aaggtgtcta cggctgcacc ttcgtcggcg agtgcacgag ggtgtgtccg aagcacgtcg
     6421 atcccgccgg cgccatccag cgctacaagc tcaaagccgc ccaggagtcg gtgaaggcgc
     6481 tgttgcttcc gaggggttcg cgatgagcga gcttcgcacg taccgatggc aggtcaagac
     6541 attctggtgg gtcaagaagt ggacgtactt cctcttcgtg atgcgcgagc tcagcagcat
     6601 cttcgtcgcg tggttcgtcc tctacctgct gatcctgatc gctgccgtca accgaggcga
     6661 cgcggcatac gaggacttca aggacttcgc gtccaacccg gcgatcgtgg tgctcaacct
     6721 cgtcgcgttc gccttcgtcg tgctgcacgt catcacctgg ttcagcctga cgccgaaggc
     6781 gatggacgtg cggctcggtg atcggccggt gccgtcctgg gcggtggtcg catcgcagta
     6841 cgccgcgctc gtcgtcgtct ccgcggtcat cctctggctg gtgaccggat gaggacccgc
     6901 gtcgagccct tcgtctggct gctgttcagc ggtggcggtg tgctggcggc ggtgttcctg
     6961 ccgatgctcg ccttcctgtt cgggctggcg tacccgctgg gcctgctgga ctggcccgag
     7021 cgcgaccacc tgaatgccgt ggtcagcaac ccgctcacga tcctggtcct gctcggcctg
     7081 ttcgtgatgc tgctcgtcca cagcgcgcac cgcttcc
//
LOCUS       DATBFZ010000308         1890 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_5684435, whole genome shotgun sequence.
ACCESSION   DATBFZ010000308 DATBFZ010000000
VERSION     DATBFZ010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 1890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1890
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5684435"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            22..456
                     /locus_tag="VH419_09390"
     CDS             22..456
                     /locus_tag="VH419_09390"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEY7043866.1"
                     /translation="MSDPSSWTAHRLTELSQTECRRLLSTKSVGRVAFAGEGAAPIVL
                     PVNYVSRDGVIYFRTASHNVLGTHLKDAMASFQVDDIDDFLESGWSVLVQGQSDFVDP
                     SDEADLGLDRPSRQPDPWASGVRNLLVRITPFEISGRRINPR"
     gene            572..1789
                     /locus_tag="VH419_09395"
     CDS             572..1789
                     /locus_tag="VH419_09395"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020579083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEY7043867.1"
                     /translation="MGSGRNRVVVVNAGSSSLKYAAFALDESGVRAGQRSTGLVEKVG
                     QDQAHLRHTRVDGSPVEDDVHAPDHATALEVMQTALRGDGVTAADVVAVGHRVVHGAG
                     GFTTATVVDAAVEAEIERLAPLAPLHNPPNLAGIRSARQAFPDVPQVAVFDTAFHATI
                     QAAAFTYALPTEFAEPLELRRWGFHGINAAYVTRTAAQWLGRPVDDVQLVICHLGNGA
                     SVTAVRDGRSVDTSMGMTTLEGLVMGTRSGDVDPAVLVRLQEEHGLSATEALDVLNHR
                     SGLLGLCGDSDMRAVCARADSGDAAAVLALSVYTHRVRKYVGAYLAELPALDALVFTA
                     GVGEHAADVRRHVVAPIAHLGLRLDDEANEAVTGPSGPVAIDDGSGGPAVLVIPADEE
                     IEIAQQTHQLLST"
BASE COUNT          294 a          650 c          653 g          293 t
ORIGIN      
        1 aggagagggg tgaggtgtgt catgtccgac ccatcgagct ggaccgcaca tcggctgacc
       61 gagctgtccc agaccgagtg tcgacgcctg ttgagcacca agtcggttgg ccgggtggcg
      121 ttcgcaggag agggggcggc gccgatcgtg ctgccggtca actacgtgtc aagggacggc
      181 gtcatctact tccgcacggc ttcgcacaac gtgctgggca cacacctcaa ggacgcgatg
      241 gcgtccttcc aggtcgatga catcgacgac ttcctggagt ccgggtggag cgtcctcgtg
      301 caagggcaga gtgacttcgt cgaccccagc gacgaggccg acctcggcct ggaccgtccg
      361 tcccggcaac cggacccgtg ggcgtccggc gtacggaatc tgctcgtgcg catcacgccg
      421 ttcgagatct ccggtcgccg gatcaaccct cgctgaggcg ccttgtccgc ggtggtggca
      481 gtcgagccct cgatcgaggt ggcactgacg tagcgacacg ccggcgaagt tgaacgaaac
      541 ttgccacctc ggcgggaagg gtggagccga catggggagc ggccggaatc gtgttgtcgt
      601 ggtgaacgcg ggctcgtcat cgctgaagta cgccgcgttc gcgttggacg agtccggcgt
      661 acgcgccggg caacggtcca caggcctggt cgagaaggtg gggcaggacc aggcccacct
      721 ccgccacacg agggtcgacg gctcacccgt ggaggacgac gtccacgcgc ccgaccacgc
      781 caccgcgctc gaggtcatgc agaccgcgct gcggggcgac ggggtgacgg ccgctgacgt
      841 ggtcgccgtc ggtcaccggg tcgtgcacgg cgcgggcggt ttcaccacgg ccacggttgt
      901 cgacgccgcg gtcgaggcgg agatcgaacg gctggcgccg cttgccccgc tgcacaaccc
      961 gccgaacctg gccggcatac ggtcggcccg acaggccttt cccgacgtcc cccaggtcgc
     1021 cgtgttcgac acggccttcc acgccacgat ccaggcagct gccttcacgt acgcgctgcc
     1081 gacagagttc gccgaaccgc tcgagctgcg ccggtggggc ttccacggca tcaacgccgc
     1141 gtacgtcacc cggactgccg cgcaatggct gggccggccg gtcgacgacg tgcaactggt
     1201 catctgtcat ctcgggaacg gcgcatcggt cactgctgtc agggacggcc gcagcgtcga
     1261 cacctcgatg ggcatgacca cgctcgaggg cctggtgatg ggcacccggt ccggtgatgt
     1321 cgacccggcc gtcctcgtcc ggttgcagga ggaacatggt ctgtcggcga cggaggcgct
     1381 cgacgtgctc aaccatcgca gcgggctcct gggcctgtgc ggcgactccg acatgcgcgc
     1441 cgtgtgtgcg cgcgccgact ccggcgacgc cgccgccgtg cttgcgctgt ccgtctacac
     1501 ccaccgggtc cgcaagtacg tcggtgccta cctggccgag ctgccggcgc tggacgctct
     1561 cgtgttcacc gccggcgtcg gcgagcacgc tgccgacgta cgacgacatg tcgtcgcgcc
     1621 aatcgcacac ctcggactgc ggctcgacga cgaagccaac gaggcagtga ccggtccgtc
     1681 aggtccggtc gcgatcgacg acgggtccgg cggaccggcg gtgctggtca tccccgccga
     1741 cgaggagatc gagatcgcgc aacagaccca ccaactcctc tccacctgac acccccgcgg
     1801 cctccggccg aggtggcaag tttggttggc aacacgccga ttgagcgaac gcctttgcca
     1861 cctcggcggt gagggtcagc cggtctcggc
//
LOCUS       DATBFZ010000309         9808 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_6341968, whole genome shotgun sequence.
ACCESSION   DATBFZ010000309 DATBFZ010000000
VERSION     DATBFZ010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 9808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9808
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6341968"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..313)
                     /locus_tag="VH419_09400"
     CDS             complement(68..313)
                     /locus_tag="VH419_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017489700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEY7043868.1"
                     /translation="MASTEEIRAGLAEIVNEVAGIPAEDVQLDKSFVDDLDVDSLSMV
                     EVVVAAEENFGVKIPDDEVKNLKTVGDAVAYIENAQS"
     gene            complement(420..1427)
                     /locus_tag="VH419_09405"
     CDS             complement(420..1427)
                     /locus_tag="VH419_09405"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEY7043869.1"
                     /translation="MTDKTIQTSTGARHAAIQGIGGYRPSRVVTNEEICQLIDSSDEW
                     IRTRSGIIERKWAEPHETVQMMSVAAARKALSRAGIDAAQVDCLIVATVSHLLQTPAV
                     ATLVSEELGCTPAAAFDISAACAGFCHGVALASDMVRSGGAKYVLAIGVERLSDITDP
                     TDRGTAFIFADGAGAAVVGPSETEGIGPVVWGADGSKFNLIRQNETWTDVLDMDTPEM
                     PTLRMDGNPVFRWASYEMAKIAGEALDRAGMSVDDLDVFVPHQANMRITDAMARALKL
                     PEHVVIARDIAYQGNTSAASIPLACEALLESGEAKSGDLALFIAFGAGLAYAAQVVTL
                     P"
     gene            complement(1432..2613)
                     /locus_tag="VH419_09410"
     CDS             complement(1432..2613)
                     /locus_tag="VH419_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012920193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HEY7043870.1"
                     /translation="MLVIVAPGQGAQTPGFLAPWLEDPGFAERLNWLSAVTGLDLAHF
                     GTDADAETIRDTAVAQPLLVASSLLAALALYPHPADAFGRIGAVSGHSVGEIAAATGA
                     GVITAEQALVFVRERGRAMAAASAKTPTGMTAVLGGDRDDVLATLEKHGLVAANDNGT
                     GQVVAAGTLEQLQALADAPPEKARLVPLSVAGAFHTEHMHPAVEVLGRLARAMSTHDT
                     RTRLISNRDGQVVHSGRDVLSRLVTQPSLPVRWDLCMQTMADLGVTGLLEMPPAGTLT
                     GIAKRQLKGVETFALKTPDQLDDARAFADKHGDASPIDSTPTWRMLVSPSKGTFHKTH
                     DIPAGAQLDPGVQVGEVENLRGKTPVEAPHGGTIVEWIVEDGDLVSPGQPLVRLHPES
                     E"
     gene            complement(2693..3853)
                     /locus_tag="VH419_09415"
     CDS             complement(2693..3853)
                     /locus_tag="VH419_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014156449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY7043871.1"
                     /translation="MAPRDDAAVRRLEQSVGSLTKAAIAKMNDDLPWFRALTAEDRSW
                     IGLIAGNGISSFVEWFSNPQRTRSGHAVPADVFGTAPRELTGVVTLQQTVEMVRTTIW
                     VVEAHVDEVVSPRDAQRVREAVMLYSREIAFAAAEVYARAAEQRGAWDARLEALIVDS
                     VLRGEADEAVRSRATALGWASTSGVCVVLGFTPVGDSAAAVDAIKRSANAHHFDALCA
                     VQGERLVVVLGGVDDPDKAASSIADYFGAGPLVTGPVVGDLLAASVSARSAIAGLRAA
                     PAWPEAPRPVSSDDLLPERSLAGDGHARRQLVSTIYQPLQDAGSGLLETLTAYLDSSG
                     SIEATARALFVHPNTVRYRLKRIADVTGMSPSEARDAYTLRVSLTLGRLIAL"
     gene            3942..5027
                     /locus_tag="VH419_09420"
     CDS             3942..5027
                     /locus_tag="VH419_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012920195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEY7043872.1"
                     /translation="MPGSPGAGEVGVVGQDEAVDGIEALSEIAYWKERGREETRRVEA
                     YRKAAWALAALPAEELQRRIADKSLTDITNVGPATSAVVLEAATGVVPKKLAELRKKG
                     ERPLAEGGEALRTAVRGDLHAHSNWSDGGSPVEEMVKAARGLGHDYLALTDHSPRLTV
                     ARGLTAERLAEQLIVVADINAQIESDGDEFRLLPGIEVDILENGGLDQTSAMLNQLDV
                     VVASVHSKLRADSETMTQRMVGGISNRFTNVLGHCTGRLVAGGRGIRPESTFDADVVF
                     AACVQFDVAVEINSRPERRDPPSRLIQLALEAGCLFSIDTDAHAPGQLAFQDYGCARA
                     VTNGIPAERIVNTWPRERLLEWTHKKR"
     gene            complement(5106..7874)
                     /gene="aceE"
                     /locus_tag="VH419_09425"
     CDS             complement(5106..7874)
                     /gene="aceE"
                     /locus_tag="VH419_09425"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020577553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HEY7043873.1"
                     /translation="MAAAEDRRPPVIHEGLPTQLPDIDPEETQEWLDSLDALIDEKGR
                     PRARYVMLKLLERARESNVGVPALRSTDYINTIPPEREPWFPGDEHIERRIRAFIRWN
                     AAVMVSRANRKGLEVGGHIATYQSAASLYEVGFNHFFRGKDHPGGGDQIFIQGHGSPG
                     IYARAFLEGRFTEEQLDAFRQEVSKGPGNGLSSYPHPRLMPDFWEFPTVSMGLTALSS
                     IYQARFNRYLHNRGTKDTSQQHVWAFLGDGEMGEPESLGAIGLAAREELDNLTFVINC
                     NLQQLDGPVRGNGKIIQELESFFRGAGWNVIKVVWGREWDELLARDVDGVLVNQMNAT
                     PDGQFQTYSVETGAYIREKFFGGDPRLRKMVQDYSDEDLRKLPRGGHDYRKVYSAFKA
                     ATEHVGQPTVILAKTIKGWTIDALEGRNATHQMKKLTKDDLKKFRDRLFLPISDGDID
                     DNDVAPFYHPGPDAPEIEYMLERRRQLGGPLPKRRVDPTPLKLPGDEVYAELKQGSGK
                     QAIATTMAFVRLLRDLMKDPEIGHRIVTIAPDEYRTFGMDSMFPTAKIYSPHGQRYES
                     VDRAMLLTYRESEQGQMLHEAISEAGAMASTIAAGTSYATHGEPMIPVYIFYSMFGFQ
                     RTGDSIWAFADQMGRGFLIGATAGRTTLTGEGLQHADGHSPLLAATNPAAIHYDPAFA
                     YEISHIVQDGLARMYGSTDDHPHGEDVFYYLTVYNEPIIQLPEPDGVDVEGILRGMHL
                     VQPADTGPGDERPHLQLLASGVALPWAMKAQRLLAEEWQVRADVWSVTSWNELARDGW
                     RAEEYNLLHPTEEHRTPYVTAKLAEAVGPVLAVSDYMRAVPDQISRWVGSSWHSLGTD
                     GFGFADTRAAARRFFKVDAESIVVGALEALAQRGDVDASRAAEAIAAYRIDDPTAVAH
                     VKQEGAGA"
     gene            8224..8655
                     /locus_tag="VH419_09430"
     CDS             8224..8655
                     /locus_tag="VH419_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016826463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3052 domain-containing protein"
                     /protein_id="HEY7043874.1"
                     /translation="MSATADHAANGPASRFGFQSGMVIQELGWDEDVDEELRSEIEKA
                     VGGELVDDRYGDVVDAVILWWREDDGDLVDGLVDSLTDLVEGGPIWLLTPKVGRPNYV
                     DASDIAQAAPTAGLATTTSFAAGPDWSAMKLVSPKSARSRR"
     gene            8741..9202
                     /locus_tag="VH419_09435"
     CDS             8741..9202
                     /locus_tag="VH419_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020577549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEY7043875.1"
                     /translation="MPLELDRPAPDFTLKNTNGEDVSLSDFRGEKNVVLVFYPFAFSR
                     VCTGELEELRDRISDFSDDRTALLAISCDHMFSLRAYAERDAYPFALLSDFWPHGAVS
                     RTYGIFNDKLGCSGRATFIIDRSGVLRWQVENEIPQARDVEAYRKILAELG"
     gene            9453..9524
                     /locus_tag="VH419_09440"
     tRNA            9453..9524
                     /locus_tag="VH419_09440"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9485..9487,aa:Val,seq:tac)
     gene            complement(9580..>9808)
                     /locus_tag="VH419_09445"
     CDS             complement(9580..>9808)
                     /locus_tag="VH419_09445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043876.1"
                     /translation="VFIVGMGIAGPLAAVSPAIDPDDAAPGAPVGLWILSGCWGVATI
                     AGILVIQGRSLLSPWLLVGLIPAACAAPFLF"
BASE COUNT         1518 a         3310 c         3355 g         1625 t
ORIGIN      
        1 cagcgacgga gtcgcgggga ccaccgcgac agaccacctg cgggactgtc tgacatcggt
       61 ggctgcttca gctctgggcg ttctcgatgt aggcgacggc gtcaccgacg gtcttgaggt
      121 tcttcacctc gtcgtcgggg atcttcacgc cgaagttctc ctccgccgcg acgacgactt
      181 cgaccatcga cagcgagtcc acgtcgaggt cgtccacgaa cgacttgtcg agctggacgt
      241 cctcggccgg gattcccgcc acctcgttga cgatctcggc gagaccggcg cggatctcct
      301 cagtgctggc catgctgttg ctccttgtct ggggcttgat tggggagtgc gtgtgctgtg
      361 gctgtgcagt gtccccggta cgtcggggat ccgcaggtgc gctgcggaaa tcagtggggt
      421 cacggcagcg tgaccacctg tgcggcgtac gcgagtccgg cgccgaacgc gatgaacaac
      481 gcgaggtcgc cgctcttcgc ctcacccgac tcgagcagcg cctcgcaggc gagcgggatc
      541 gaggcagcag acgtgttgcc ctggtaggcg atgtctcggg cgatgaccac gtgttccggc
      601 agcttcaggg ctcgtgccat cgcgtcggtg atccgcatgt tggcctggtg cgggacgaac
      661 acgtccaggt cgtcgacgct catcccggcg cggtcaagtg cctcgccggc gatcttggcc
      721 atctcgtacg acgcccagcg gaacaccggg ttgccgtcca tccgcagcgt cggcatctcc
      781 ggcgtgtcca tgtcgaggac gtctgtccag gtctcgttct gacggatcag gttgaacttc
      841 gagccgtcgg cgccccacac cacggggccg atgccttcgg tctcggacgg accgacgacg
      901 gccgcaccgg cgccgtcggc gaagatgaag gcggtccctc gatcggtcgg gtcggtgatg
      961 tcggacaggc gttcgacgcc gatggcgagc acgtacttgg cgccgccgga gcgcaccatg
     1021 tccgacgcca gcgccacgcc gtggcagaac ccggcgcagg cggcgctgat gtcgaaggcg
     1081 gccgccggcg tacagcccag ctcctcggac accaaggtgg ccaccgccgg cgtctgcaga
     1141 aggtgggaga cggtggcgac gatcagacag tcgacctgtg cggcgtcgat gcccgcccgc
     1201 gacaacgcct tgcgagcggc ggcgacgctc atcatctgca cggtctcgtg cggctccgcc
     1261 cacttgcgct cgatgatccc ggagcgggtg cggatccact cgtcactgga gtcgatgagc
     1321 tgacagatct cctcgttggt gacgacgcgg ctgggccggt acccgccgat cccctggatc
     1381 gcggcgtgcc gggcaccggt gctggtctgg atcgtcttgt cggtcatggc gtcactcgct
     1441 ttcggggtgc agccggacca gcggctgacc aggggagacc aggtcgccgt cctcgacgat
     1501 ccactcgacg atggtgccgc cgtgcggtgc ctcgaccggc gtcttgccac gcaggttctc
     1561 cacctcgccc acctggacgc cgggatcgag ctgtgcaccg gcggggatgt cgtgggtctt
     1621 gtggaacgtc cccttggagg gtgagacgag catccgccag gtcggcgtcg agtcgatggg
     1681 cgacgcatcg ccgtgcttgt cggcgaacgc gcgcgcgtcg tcgagctggt caggcgtctt
     1741 gagggcgaac gtctcgacgc ccttcagctg ccgcttggcg ataccggtca acgtgccggc
     1801 gggcggcatc tcgaggagtc cggtgacccc caggtccgcc atcgtctgca tgcacaggtc
     1861 ccagcgcacg ggcaggctcg gctgcgtgac cagccggctc agcacgtcgc ggcccgagtg
     1921 gaccacctgg ccgtccctgt tggagatgag gcgcgtccgg gtgtcgtgcg tcgacatggc
     1981 gcgggccagc cgcccgagca cctcgacggc tgggtgcatg tgctcggtgt ggaaggcccc
     2041 ggccacgctc aacggaacca gccgcgcctt ctcggggggc gcatcggcca gcgcctggag
     2101 ctgttcgagc gttccggcgg caacgacctg accggtgccg ttgtcgttgg ccgcgaccag
     2161 accgtgcttc tcgagtgtcg cgagtacgtc gtcccggtcg ccgccgagga ccgcagtcat
     2221 cccggtcgga gtcttcgcgg acgcggccgc catcgcgcga ccacgctcgc gcacgaagac
     2281 caacgcctgc tcggcggtga tgacgccggc acctgttgcc gccgcgatct cgccgacgct
     2341 gtggccagag accgcaccga tccggccgaa cgcgtcggcc gggtgggggt agagcgcgag
     2401 cgccgccagc aggctcgagg cgaccagcag cggttgcgcc accgccgtgt cgcggatcgt
     2461 ctccgcgtcc gcgtcggtgc cgaagtgggc gaggtcgagc ccggtcaccg ccgacagcca
     2521 gttcagccgc tcggcgaagc ccgggtcctc gagccagggg gcgagaaagc cgggcgtctg
     2581 ggcgccctgc ccgggagcga cgatgacgag cacacggcta tcttgccgaa tcccgctgcc
     2641 gcacccgggg tcgcgcgcgc acgaaacttc agcgtgtgtc gttggaggtt tcctacaagg
     2701 cgatcagcct tcccagtgtc agcgacacgc gcagtgtgta agcgtcccgc gcctccgaag
     2761 gcgacatacc ggtcacgtcg gcgatccgct tgagccggta gcggaccgtg ttcggatgca
     2821 cgaacagcgc acgcgctgtc gcctcgatcg aaccggagct gtcgaggtac gccgtcagcg
     2881 tctccagcag gcccgatccg gcatcctgca gcggctgata gatggtcgag accagctgcc
     2941 gccgagcgtg tccgtcgccc gccaacgagc gttccggcag caggtcgtcg ctcgacaccg
     3001 gccgcggcgc ctcgggccag gcaggggccg cgcggagtcc ggcgatggcg gagcgagccg
     3061 agacactggc cgccagcagg tcgcccacga ccggcccggt gacgagcgga ccggcgccga
     3121 agtagtcggc gatcgagctc gcggccttgt cagggtcgtc cacgccgccg aggacgacaa
     3181 ccagccgctc tccctggacg gcgcacagcg cgtcgaagtg atgggcgttc gccgaccgct
     3241 tgatcgcgtc gacggcggcc gcggagtcgc cgaccggtgt gaacccgagg acgacacaga
     3301 cgccgctggt cgacgcccag ccgagcgcgg tggcccgcga ccgcaccgcc tcgtcggcct
     3361 caccgcggag caccgagtcc acgatgagcg cttcgagccg cgcgtcccag gcacctcgtt
     3421 gttcggcggc gcgggcgtag acctctgcag cggcgaaggc gatctcgcgt gagtagagca
     3481 tgacggcctc acggacccgc tgcgcatctc gcgggctgac gacctcgtcg acgtgcgcct
     3541 cgaccaccca gatcgtcgta cgcaccatct cgacggtctg ttgcaaggtc acgacgccgg
     3601 tcagctcgcg aggcgccgta ccgaacacgt cggctgggac ggcatgcccg gatctcgtcc
     3661 gttgcgggtt gctgaaccat tccacgaacg acgagatgcc gttgccggcg atcaggccga
     3721 tccacgaccg gtcctcggcg gtgagcgcgc ggaaccacgg caggtcgtcg ttcatcttcg
     3781 cgatcgcggc tttggtcaac gacccgaccg actgctcgag ccggcggacc gcggcgtcgt
     3841 cgcgaggagc catgacgtga gcctacgagc atgctttgtg acccttcaac aaatcgcccg
     3901 cctttgatcc gccaccgcca acctcctcgt atcctccgga catgccgggg agcccggggg
     3961 caggggaggt cggggtggtc gggcaggatg aagccgtgga cgggatcgag gcgctgtcgg
     4021 agatcgccta ctggaaggaa cgcggtcgcg aggagacccg ccgggtcgag gcctaccgca
     4081 aggcggcctg ggcgttggcc gcgttgccgg ccgaggagct ccaacgccgg atcgccgaca
     4141 agtcgctgac cgacatcacg aacgtcgggc ctgccacgtc ggcggtcgtc ctcgaggcag
     4201 ccaccggcgt cgtaccgaag aagctcgccg agctgcgcaa gaagggcgaa cgtccgctcg
     4261 ccgagggcgg cgaggcgttg cgtacggcgg ttcggggcga cctgcacgcg cactcgaact
     4321 ggtcggacgg cgggtcaccg gtcgaggaga tggtgaaggc cgcccggggg ctcggacacg
     4381 actacctcgc gctgaccgac cactcgccgc ggttgacggt ggcccgtggt ctcaccgctg
     4441 aacggttggc cgagcagctg atcgtcgtgg ccgacatcaa tgcgcagatc gagtcggatg
     4501 gcgacgagtt ccggctgctg cccggcatcg aggtcgacat cctggagaac ggtggcctcg
     4561 accagacctc ggcgatgctg aaccagctcg acgtcgtggt ggccagcgtg cactccaagc
     4621 tccgcgccga ctccgagacc atgacgcaac gcatggtcgg cggcatctcg aacaggttca
     4681 ccaacgtgct cggccactgc acgggtcgcc tcgtcgcggg tggtcgcggg atccggccgg
     4741 agtcgacgtt cgacgccgac gtggtgttcg ccgcgtgtgt ccagttcgac gtggcggtcg
     4801 agatcaactc ccgacccgaa cggcgcgacc ctccctcgcg gctgatccag ctcgcgctcg
     4861 aagccggctg cctcttctcg atcgacaccg acgcgcatgc gcccggccag ctggcgttcc
     4921 aggactacgg atgtgcccgg gcggtgacga acggcatacc ggcggagcgg atcgtcaaca
     4981 cctggccgcg cgaacgcctc ctcgagtgga cccacaagaa gcgctgaggt ttgccgactt
     5041 gtcagaacgc catcgaccta tagccccacc cacttctgac aactcggcgc tgctccacca
     5101 gtgacctagg cgccggcgcc ctcctgcttg acgtgcgcaa cggcggtggg gtcgtcgatc
     5161 cggtacgccg cgatcgcctc ggccgcgcgg gaggcgtcga cgtcgccacg ttgcgcgagc
     5221 gcctcgagcg caccgacgac gatcgactcg gcgtcgacct tgaagaagcg gcgcgccgcc
     5281 gctcgggtgt cggcaaaccc gaacccgtcc gtccccaacg agtgccagga cgagcccacc
     5341 caccgggaga tctggtccgg caccgcacgc atgtagtcgc tcaccgccag caccggccca
     5401 actgcctcgg ccagcttcgc ggtgacgtac ggcgtccggt gttcttcggt cgggtgcagc
     5461 aggttgtact cctcggcacg ccacccgtcc cgggcgagtt cgttccagga cgtcaccgac
     5521 cagacgtcag cgcggacctg ccactcctcg gccaacagcc gctgcgcctt catcgcccac
     5581 ggcaacgcca ctccggaggc gagcagctgg agatgcggcc gctcgtcgcc cggtccggtg
     5641 tcggccggct gcaccagatg catcccgcgc aggatgccct cgacgtcgac gccgtcaggc
     5701 tccggcagct ggatgatcgg ctcgttgtag acggtcaggt agtagaagac gtcctcaccg
     5761 tgcgggtggt cgtcggtcga cccgtacatg cgggcgagac catcctggac gatgtggctg
     5821 atctcgtagg cgaaggcggg gtcgtagtgg atggccgccg ggttggtggc cgccaacaac
     5881 ggcgagtgcc cgtcggcgtg ctgcaggccc tcgccggtca acgtcgtacg tcccgcggtt
     5941 gcgccgatca agaacccgcg acccatctgg tcggcgaagg cccagatcga gtcaccggtc
     6001 cgttggaagc cgaacatcga gtagaagatg tacaccggga tcatcggctc gccgtgcgtc
     6061 gcgtacgacg tgccggccgc gatcgtcgaa gccatggcac cggcttcgct gatggcctcg
     6121 tgcagcatct ggccctgttc ggactcgcgg tacgtcagca gcatcgcccg gtcgacactc
     6181 tcgtagcgct gcccgtgcgg tgagtagatc ttcgccgtcg ggaacatcga gtccataccg
     6241 aacgtgcggt actcgtcggg ggcgatggtc acgattcggt ggccgatctc cgggtccttc
     6301 atcaggtctc gcagcagccg gacgaacgcc atcgtggtgg cgatcgcctg ctttcccgaa
     6361 ccctgcttca gctcggcgta gacctcgtcg ccgggaagct tcaacggcgt cgggtcgact
     6421 cgccgcttcg ggagtggccc gccgagctgt cgacgccgct cgagcatgta ctcgatctcg
     6481 ggcgcgtccg gccccgggtg gtagaacggc gcgacgtcgt tgtcgtcgat gtcgccgtcg
     6541 ctgatcggga ggaagaggcg gtcccggaac ttcttgaggt cgtccttggt cagcttcttc
     6601 atctggtgcg tggcgttgcg gccctcgagt gcgtcgatcg tccaaccctt gatggtcttc
     6661 gccaggatca ctgtcggttg gccgacatgc tcggtcgcgg ccttgaacgc cgagtagacc
     6721 ttgcggtagt cgtggccacc tcgtggcagc ttgcgcaggt cctcgtcgga gtagtcctgg
     6781 accatcttgc gaagccgggg gtcgccgccg aagaacttct cccggatgta ggcgccggtc
     6841 tcgacggagt acgtctggaa ctgcccgtcc ggcgtggcgt tcatctggtt gaccagtacg
     6901 ccgtcgacgt ctcgcgcgag cagctcgtcc cactcgcggc cccacaccac cttgatgacg
     6961 ttccagccgg caccgcggaa gaacgactcc agctcctgga tgatcttgcc gttgccgcgc
     7021 accggtccgt cgagctgctg caggttgcag ttgatgacga acgtcaggtt gtcgagctct
     7081 tcgcgggccg ccagtccgat ggcgccgagc gactcgggct cacccatctc accgtcgccg
     7141 aggaacgccc agacgtgctg ctgcgacgtg tccttggtcc cgcggttgtg caggtaccgg
     7201 ttgaacctcg cctggtagat cgagctcagc gcggtcaagc ccatcgagac ggtcgggaac
     7261 tcccagaagt cgggcatcag ccggggatgc gggtacgacg aaagcccgtt gccgggaccc
     7321 ttcgacacct cctgccggaa cgcgtcgagc tgttcctccg tgaaccgtcc ttcgaggaag
     7381 gccctcgcat agatcccagg tgacccgtgg ccctggatga agatctggtc accaccaccc
     7441 ggatggtcct tgccacggaa gaagtggttg aagccgacct cgtagaggct cgccgccgac
     7501 tggtacgtgg cgatgtgacc gcccacctcg agacccttgc ggttcgcgcg cgacaccatg
     7561 acggcggcgt tccaccggat gaaggccctg atccgtcgtt cgatgtgctc gtcaccgggg
     7621 aaccacggct cgcgctccgg cgggatcgtg ttgatgtagt cggtgctgcg cagcgccggg
     7681 acgccgacgt tgctctcccg agcacgctcg agaagcttga gcatgacgta ccgcgcgcgg
     7741 ggacggccct tctcgtcgat cagcgcgtcg agcgagtcga gccactcctg ggtctcctcg
     7801 gggtcgatgt cgggaagctg cgtcggcaga ccctcgtgga tcaccggcgg gcgacggtct
     7861 tcagcggcag ccacggcgtg tcctcttcat cgttcggggg tcgtggccgt cgtacgccgt
     7921 acggtccgac cacgacccgg gtactgcagg acgatgagcc gaccaggtgt cctgctcgtt
     7981 gcatcctcgc aggtttgtcc gcggggctca tctcggtagc tccagcctag gcgctcatcc
     8041 agcggtcgac cgtgccgagg ccccgatccc gacgcggccg atcagcaggt cctccgaacg
     8101 ggctcaaccg gcggggtttc ctcgaagggc ttgcatagac gcccagttca cggtggacta
     8161 ccccgcatcg agggtgcttc gccgggagtc aggcggcccc cagttcgcca agggaggcat
     8221 cgggtgagcg cgaccgcgga ccacgcggcc aacggaccgg cgagccggtt cggattccag
     8281 tccggcatgg tgatccagga actgggttgg gacgaggacg tcgacgagga actgcgatcc
     8341 gagatcgaga aagccgtcgg cggtgaactg gtcgatgacc ggtacggcga cgtggtggac
     8401 gcggtgatcc tgtggtggcg tgaggacgac ggcgacctgg tggacggact ggtcgactcg
     8461 ctcaccgacc tggtcgaggg cgggcccatc tggctgctca ctccgaaggt gggtcgtccc
     8521 aactacgtcg acgccagcga catcgcacag gcggccccga ccgcaggcct ggcgacgacg
     8581 accagtttcg cggccggacc cgactggtct gccatgaaac tcgtctcgcc gaagtcggcg
     8641 cggtcgcgtc gctgacctct caccagcgcc gctccagcgc ggctcgtcgc tgtgctggat
     8701 gatgatggtg accatcgaac ctgacgagaa tcgagcactc atgcccctcg agctggaccg
     8761 gccggcgccg gacttcacgc tcaagaacac caacggtgaa gacgtctcgc tgtcggactt
     8821 ccgtggcgag aagaacgtcg tcctcgtctt ctaccccttc gcgttcagcc gggtgtgcac
     8881 cggcgagctg gaggagttgc gtgaccggat ctcggacttc tccgacgacc ggacggcgct
     8941 gctggcgatc tcctgcgacc acatgttctc gcttcgtgcg tacgccgagc gcgacgctta
     9001 cccctttgcg ctgttgagcg acttctggcc gcatggtgcg gtgtcgcgga cgtacggcat
     9061 cttcaatgac aaactcggat gttctggacg ggcgacgttc atcatcgatc gttccggtgt
     9121 gctgcgctgg caggtggaga acgagatccc gcaggcccgc gacgtggagg cgtaccgcaa
     9181 gattctcgcc gagctcggct gaccgcgctc gacaccggcc gacacgtatc gtcacgcgcc
     9241 gacacggggg gtgcgatttg cttccagcgt ggaccgcgtt gtcgcaggtg ggaacgcaga
     9301 tttcttgggc tcggtttggt caaagcgctg gcgcccagcc ggatcgcagt gttcgataga
     9361 tgtcaagaca cgaccgccag cgaaccgaga cgttggcggt cgttgtcttt tgtcgccggc
     9421 ggccggcggg cgttacgatt tccgccgctc gagggcgtat agctcagcgg tagagcacct
     9481 gccttacaag caggtggtcg caggttcgat ccctgctgcg cccaccggtt ccggacccgg
     9541 aagcgtccgt ccgggccata ccgtcgccct tgccgaaggt cagaacagga acggcgcggc
     9601 gcacgcagcc gggatgagtc cgaccagcag ccatggcgac aacagcgagc gaccttggat
     9661 gaccaggatg ccggcgatgg tcgcgacccc ccagcagccc gacaggatcc acaggcctac
     9721 cggcgctccg ggggccgcgt cgtccgggtc gatcgccgga ctcaccgcag ccaacgggcc
     9781 ggcgatgccc atgccgacga tgaagacc
//
LOCUS       DATBFZ010000310         9859 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_6342110, whole genome shotgun sequence.
ACCESSION   DATBFZ010000310 DATBFZ010000000
VERSION     DATBFZ010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 9859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9859
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6342110"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="VH419_09450"
     CDS             complement(<1..186)
                     /locus_tag="VH419_09450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043877.1"
                     /translation="MAGLPRSVRVPRNPVARALRRSGLSAVRQLRVAETTWLVVLCAL
                     ALLMVLCQREFPEWWPLT"
     gene            complement(224..1039)
                     /locus_tag="VH419_09455"
     CDS             complement(224..1039)
                     /locus_tag="VH419_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014167025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HEY7043878.1"
                     /translation="MPEGHTLFRLAADLSAEFGGHHVDVWSPQGRFSEAAARVDGHVL
                     ESAESAGKHLFIRFDDASTIIHVHLGLIGVFTVQDGPPPEPVGAVRLRIVGPAAYADL
                     RGATTCELISEDAMTTILANLGPDPLRPDADPDKAFARIRKSKKPIGALLMDQSVLAG
                     VGNVYRAESLFRARLHPLTPGVKVAKRRWDRIWVDLVDLMEYGVQTGRIDTVRPEHEP
                     HVMGRDPRVDDHGGEVYVYRRNDQACFVCGSTVRTRLLEGRNLFWCPTCQRRR"
     gene            complement(1053..1556)
                     /locus_tag="VH419_09460"
     CDS             complement(1053..1556)
                     /locus_tag="VH419_09460"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020391463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase"
                     /protein_id="HEY7043879.1"
                     /translation="MRVHLGSDHAGLELKAHLVGWLEEHGHQVIDHGTYEYDDGDDYP
                     PFCFRTGLAVVADPGSLGVVIGGSGNGEQMAANKVAGIRAALAWSEETAKLARLHNNA
                     NVVALGARMHDVDEATALVEIFLDTDFTGEQRHVRRIAMLTDYEESGVIPEVPVADLG
                     SSGGSAV"
     gene            complement(1569..2468)
                     /locus_tag="VH419_09465"
     CDS             complement(1569..2468)
                     /locus_tag="VH419_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017934183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY7043880.1"
                     /translation="MVLHPQARRAVAMWALDPPPESPDFDVQVRRAAALEIARSAPRE
                     PVEAVVDLEADGVPCRLYRPRAGAPLMVGLHGGGFVMGELETHDGQWRRVANRTGWAV
                     LAVDYRRAPEHRYPAAPDDVDTAITWARSNAGQLSVDATRIAVAGDSAGGWLALVAAL
                     RNPGLLAVGLIYPCLDPAGSQPSYRSETGGLTGAGMDWYWDQFLGDDRSSAELDLLNA
                     DLSGLPPTWVLTAEHDPLRDEAELLGRRLADAGVTTTSTRFLGMVHGFWRWPELFDAS
                     DVAVRQLGAFLDGAVEEEPRTSI"
     gene            complement(2502..3146)
                     /locus_tag="VH419_09470"
     CDS             complement(2502..3146)
                     /locus_tag="VH419_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007078344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEY7043881.1"
                     /translation="MSTAPANDSEPTTTPVVADFWFDPACPWAWITSRWMLEVEQVRD
                     VRVDWHVMSLAVLNEHKEGLSDKYRDSLKTMWGPVRVCIAAAERHGEQALGRLYTELG
                     TRFHHEKQERDRATVAAALEAAGLDADLAEAMDATEYDEALRKSHHAGMDQVGDDVGT
                     PVISVEGAAFFGPVVTPIPRGEDAGRLWDGVRLVSSVEGFYELKRSRDARPSFD"
     gene            3265..5808
                     /gene="pepN"
                     /locus_tag="VH419_09475"
     CDS             3265..5808
                     /gene="pepN"
                     /locus_tag="VH419_09475"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012919880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HEY7043882.1"
                     /translation="MPGINLTRDEAAERARLLDVSTYDVSLDLTTSETTFGSTTVIRF
                     SCAEPGASSFADLVGAQIHEITLNGRSIDTSAYADSRIPLTDLQADNELRVVADCTYS
                     HVGEGLHRFVDPADKLVYLYTQFEVPDARRVFTTFEQPDLKGEFTFHVTAPSHWEVVS
                     NSATPEPEQAGLGKSVWHFPPTKRLSTYITALVAGEYAVLRDTYHGKRGDIPLGIFLR
                     ESLLQYLDADDIFEVTKQGFEYFEDRFDMAYPFGKYDQLFVPEYNMGAMENAGCVTFR
                     DEYIFRSRQTRAAYEGRANTILHEMAHMWFGDLVTMKWWDDLWLNESFAEWASHQAAT
                     DATQYDGAWTNFCNGRKTWAYRQDQLPSTHPIAADNYDLEAVEVNFDGITYAKGASSL
                     RQLVAWVGQDEFFAGLRAYFERHAFGNTELTDLLKALEETSGRDLGSWTKEWLQTSGV
                     NTVRPRFEVDESGAFTSFAVEQTAHPEFPTLRRHRLAVGLYDLTDAGLVRRTSVETDV
                     SGAETYVAELVGERQPDLVLLNDGDLTYAKVRLDERSLATVVANIDRFTQSLPRAVSW
                     GAAWDMTRDAEMRASDWVELVLRGIRVETDQFAVRSLVARTGQAISQYTAPGNRRAVA
                     ARWETGLLALVRDAEPGSDQQLAFVRGLAQAAHNDEALGLVAGLLAGSETLDGLSVDT
                     DLRWQLLNGLAREGRADDEAIDQELDRDMTISGQEEAASARASMPTAEAKAHAWREAV
                     ENRETANETMRSIAVAFNQPGQEEVLRDYVPKYLTTAETIWEERGVYVASTILSNMFP
                     SSIPTQETLDTVDAWLATTKANPAARRLVSEGRDDVARALAAQTRDSHP"
     gene            complement(5833..6222)
                     /locus_tag="VH419_09480"
     CDS             complement(5833..6222)
                     /locus_tag="VH419_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012919875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5130 family protein"
                     /protein_id="HEY7043883.1"
                     /translation="MPAGDAFTAVQRNEIAKAIADAERVSGRTFSVHVGPSTSDPREY
                     AERLHAALDDPSTSVLIHVDPAARVLEIVTGSEVRRTLTNHQAALAAINMQSAFATGD
                     LTRGLLAGLQQLAPLAKPEHSLHTDTP"
     gene            complement(6209..6517)
                     /locus_tag="VH419_09485"
     CDS             complement(6209..6517)
                     /locus_tag="VH419_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012919874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043884.1"
                     /translation="MTPLADTPTTWPDPEPMSTLHAVLFFVGIPLLIIAVITLLVMAP
                     SLAKGPRYRLTEQWDANAEWFGAPSAVEAAAEAPELEASKVGSEPPRADDSGGASASW
                     "
     gene            6645..7640
                     /locus_tag="VH419_09490"
     CDS             6645..7640
                     /locus_tag="VH419_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEY7043885.1"
                     /translation="MAASDACDTGDDLCRYVANLTNSEGLAKASNWLIAKPAAILALI
                     LGGLIIRWLLHRAIDRLARRAAAGTVPGVIARGKVPQMFLEENAAIAERRKQRAETMG
                     SLLKSITTGVVLSIVAIMVIAQLGYNIAPLIASAGIIGVALGFGAQSLVKDFISGIFM
                     ILEDQYGVGDSITVGEVSGTVEAVGLRVTRIRDVNGTVWYQRNGEILAVGNMSQNWAR
                     TVLDIPVAFSEDLARIREMLHEVAQDLWEDPEFQGDILEEPEVWGVERWDPDGVVVRV
                     VLKTAPLRQWTVARAMRERIKDRFDAEGVEIPLPQRMVWHRDASAPSDTAVADAP"
     gene            complement(7637..8065)
                     /locus_tag="VH419_09495"
     CDS             complement(7637..8065)
                     /locus_tag="VH419_09495"
                     /EC_number="1.11.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0033194 - response to hydroperoxide
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEY7043886.1"
                     /translation="MATTRTANAHWEGSLIEGSGNVSLDSSNLGSFDVTWASRAEAPD
                     GRTSPEELIAAAHSTCFSMALSHGLAGNGHPPQQVDTKADVDFVAGTGITEIRLTVRA
                     RVEGLSNDEFVAAAEDAKVNCPVSQALTGTTIKLDAALAD"
     gene            8162..8605
                     /locus_tag="VH419_09500"
     CDS             8162..8605
                     /locus_tag="VH419_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020574117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="globin"
                     /protein_id="HEY7043887.1"
                     /translation="MTAREEHVEQTEQSFYDAVGGHETFVRLVDRFYAGVAEDPVLRS
                     LYPEADLGPATTRLRMFLEQYWGGPTTYSDTRGHPRLGMRHAPFAVTPTARDHWLAHM
                     RVAVEESGMSPEHQDQFWQYIVRAAHFLVNTFDEEPPTTSGRSLL"
     gene            complement(8620..9054)
                     /locus_tag="VH419_09505"
     CDS             complement(8620..9054)
                     /locus_tag="VH419_09505"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEY7043888.1"
                     /translation="MTRHVFNLKPRWSDLDAYGHVNNVTWLEYLQEARVDMLFVHAPA
                     RGAEGLAEGVLVVRAEIDYRRAMLFRQEPYPIELWVSKVGGASFTISYEIAEPGPDEK
                     RILYGRAATVLAPVDMKTGAPRRLTEIERDVLNRFMRDATQG"
     gene            9166..>9859
                     /locus_tag="VH419_09510"
     CDS             9166..>9859
                     /locus_tag="VH419_09510"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012919852.1"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEY7043889.1"
                     /translation="MPEAVIVATARSPIGRAGKGSLVDVRPDDLTAAMVAAALAKIPQ
                     LDAHDIDDLMLGCGLPGGEQGFNMARVVAILLGYDQLPGTTLTRYCASSVQTSRMAFH
                     AIKAGEGEVFVSAGVETVSRYVNGSSDSHPGTQNPVFDDARARTRAAAEGGAQPWHDP
                     RADGILPDIYMPMGQTAENLAQSRGISRQELDEFGVRSQNLAEKAIAGGFWEREIVPY
                     TTPDGTVVDKDDG"
BASE COUNT         1623 a         3372 c         3314 g         1550 t
ORIGIN      
        1 ggtgagcggc caccattccg ggaactcgcg ctggcagagc accatgagca gggcaagggc
       61 gcacaacacc accagccatg tggtctcggc aacccgcagc tggcgaaccg ccgacagccc
      121 gcttctgcga agagcccgcg caaccgggtt gcgaggcact cgcacggagc gcggaaggcc
      181 tgccacggct cagagagtaa ccaacccagg ctgcccggcg ctgtcagcgc cgccgttggc
      241 aggtggggca ccagaacagg tttcgtcctt ccagcagcct ggtccggacg gtcgacccac
      301 agacgaagca cgcctggtcg tttcggcgat agacgtacac ctcgccgccg tggtcgtcga
      361 cccgcgggtc acgacccatc acatgcggct cgtgctcagg ccggacggtg tcgatgcgac
      421 cggtctgcac gccgtactcc atcagatcca cgagatcgac ccagatccgg tcccatcgcc
      481 gcttggccac cttgacgcca ggtgtgaggg gatggagtcg agcgcgaaac agcgactccg
      541 cccggtagac attgccgacg ccggccagca cggactggtc catcaggagt gcgccgatcg
      601 gcttcttgct cttgcgaatt ctcgcgaacg ccttgtcggg gtcggcgtcc ggacgcagcg
      661 gatccggacc gaggttcgcc aggatcgtcg tcatcgcgtc ctccgagatg agctcgcacg
      721 tcgtcgcgcc gcggaggtcg gcgtacgccg ctggaccgac gatgcgcagc cgcacggcac
      781 cgaccggctc cggcggcggg ccgtcctgca cggtgaacac gccgatcagt ccgaggtgga
      841 cgtggatgat cgtggaggcg tcgtcgaagc ggatgaacag atgcttgccg gcggactcgg
      901 ccgactccag gacgtgaccg tcgacgcgtg ccgccgcctc gctgaaccgg ccctgcgggc
      961 tccagacgtc gacatgatgg ccgccgaact ccgctgacag gtcggcggca agacgaaaga
     1021 gcgtgtgtcc ctcaggcatg gagcagtacg tcctagacgg cgctgccgcc ggacgaaccc
     1081 aggtctgcga cggggacctc ggggatgacg ccgctctcct cgtagtcggt gagcatcgcg
     1141 atccggcgga cgtgccgctg ctcgccggtg aagtcggtgt ccaggaagat ctcaaccagc
     1201 gcggtggcct cgtcgacgtc gtgcatgcgc gctccgaggg ccacgacgtt ggcgttgttg
     1261 tgcagccgcg cgagcttcgc cgtctcctcc gaccaggcga gcgcggcgcg gatacctgcg
     1321 accttgttgg cagccatctg ctcgccgttg cccgaaccgc cgatcaccac accgaggctt
     1381 cccggatccg cgaccacggc gagaccggtg cggaagcaga acggcgggta gtcgtcaccg
     1441 tcgtcgtact cgtacgtgcc gtggtcgatg acttggtggc catgctcctc cagccagccg
     1501 acaaggtgag ccttgagctc caacccggcg tggtcggacc cgaggtgaac gcgcatgacg
     1561 tgatcctgtc agatggacgt tcgcggctct tcctcgacgg cgccgtcgag aaaggcgcca
     1621 agctgccgga cggcgacgtc ggaggcgtcg aagagctccg gccagcgcca gaacccgtgg
     1681 accatcccga ggaaccgcgt gctcgtggtt gtcacgccgg cgtcggccag tcgccggcca
     1741 agcagctctg cctcgtcgcg cagcgggtcg tgctccgcgg tgagcaccca ggtcggcggg
     1801 agtccgctga ggtcggcgtt caacaggtcg agctcggcgg atgagcggtc gtcgccgagg
     1861 aactggtccc agtaccagtc catcccggcc ccggtcaggc caccggtttc gcttcggtac
     1921 gacggctgcg agccggccgg gtcgagacac gggtagatca ggccgaccgc cagcaggccg
     1981 gggttgcgca gagcagcgac gagggccaac cacccgcctg cggagtcgcc cgcaacggca
     2041 atacgggttg cgtcgaccga cagctgaccg gcgttgctgc gggcccaggt gatcgcggtg
     2101 tccacgtcgt cgggcgccgc ggggtagcgg tgctcggggg cgcgacggta gtcgacggcg
     2161 agcaccgccc aaccggtccg gttggcgacc cggcgccact ggccgtcgtg ggtctcgagc
     2221 tcccccatga cgaatccgcc gccgtggagc ccgaccatca gcggcgcgcc agcgcgcggc
     2281 cgatagagcc ggcacggaac tccgtcagcc tcgaggtcga cgacggcttc gaccggttcc
     2341 cgcggtgccg atcttgcgat ctccaaggca gcagctcggc gtacctgcac gtcgaagtcg
     2401 ggcgactccg gcggcgggtc gagcgcccac atcgccaccg cccgcctcgc ctgcggatgc
     2461 agcaccatct tgcgagcgta tccaggtgca cggatggacg gtcagtcgaa agaggggcgc
     2521 gcgtcgcggc tgcgcttcag ctcgtagaaa ccttcgacgc tggacacgag ccggaccccg
     2581 tcccacagcc ggcctgcgtc ctcaccgcgt gggatgggtg tcacgaccgg accgaagaac
     2641 gccgcaccct cgaccgagat gaccggcgta cccacgtcgt caccgacctg gtccatgccg
     2701 gcgtggtgcg acttccgcag cgcttcgtcg tactccgtcg cgtccatcgc ctcagccaga
     2761 tccgcgtcca ggcccgctgc ctccaacgcc gctgcgacgg tggctcgatc gcgctcctgc
     2821 ttctcgtggt ggaagcgcgt gccgagctcg gtgtagagcc gtccgagagc ctgttcgccg
     2881 tgccgttcgg cggcggcgat gcagacgcgc accggacccc acatcgtctt cagcgaatcg
     2941 cggtacttgt cgctgaggcc ctccttgtgc tcgttcaaca cagccaggct catgacgtgc
     3001 cagtcgaccc tgacgtcgcg gacctgctcg acctccagca tccagcggga ggtgatccat
     3061 gcccacggac aggccgggtc gaaccagaag tctgcgacga ccggggtcgt ggtgggctcg
     3121 gagtcattcg cgggtgcggt cgacatgtca ctcctcggga gccggtgcct gctgtgcgcg
     3181 ggcacagcat acgtcctgtc gaccctgtcg caaccgcagg aaccatttgc ggcattcccc
     3241 caacccgcgt gcgaggatga cgccatgcct gggatcaacc tgacacgcga cgaggcggcc
     3301 gagcgagcac gcctgctcga cgtctccacg tacgacgtca gcctcgatct gaccacctcc
     3361 gagaccacct tcgggtccac gaccgtgatc cggttctcgt gcgccgagcc cggtgcttcg
     3421 tcgttcgccg acctggtcgg ggcgcagatc cacgagatca cgttgaacgg acgctcgatc
     3481 gacacctctg cgtatgccga cagccggatc ccgctgaccg accttcaggc tgacaacgag
     3541 ctccgagtgg ttgccgactg cacctacagc cacgtcggcg agggtctgca ccggttcgtc
     3601 gaccctgccg acaagctcgt ctacctctac acgcagttcg aggtgcccga cgcccggcgc
     3661 gtgttcacga cgttcgagca gccggatctg aagggcgagt tcacctttca cgtgaccgcg
     3721 ccatcccact gggaagtcgt gtccaactcg gcgacgccgg agcccgagca ggccggtctc
     3781 gggaaatcgg tgtggcactt cccgccgacc aagcgcttgt cgacctacat caccgctttg
     3841 gtggccggcg agtacgccgt cctgcgcgac acctaccacg gcaagcgtgg agacatcccg
     3901 ctggggatct tcctgcgtga gtcgctcctc cagtacctcg atgccgacga catcttcgag
     3961 gtcaccaagc agggcttcga gtacttcgag gaccggttcg acatggcgta cccgttcggg
     4021 aagtacgacc agctgttcgt gccggagtac aacatgggcg cgatggagaa cgccggctgt
     4081 gtgacgttcc gcgacgagta catcttccgc agccggcaga cccgggcggc gtatgagggc
     4141 cgcgcgaaca cgatcctgca cgagatggcg cacatgtggt tcggcgatct cgtcacgatg
     4201 aagtggtggg acgacttgtg gctgaacgag tcgttcgccg agtgggcgtc gcaccaggcc
     4261 gccacggatg ccacccagta cgacggggcg tggacgaact tctgcaacgg ccgcaagacc
     4321 tgggcgtacc gacaagacca gctgccgtcg acccacccga tcgccgccga caactacgac
     4381 ctcgaggcgg tcgaggtgaa cttcgacggc atcacctacg ccaagggggc gtcgtcgctg
     4441 cggcagctgg tggcgtgggt cggtcaggac gagttcttcg cggggctgcg cgcgtacttc
     4501 gagcggcacg cgttcgggaa caccgagctc accgacctgc tcaaggcgct ggaggagacg
     4561 tccggacgcg acctcgggtc gtggacgaag gagtggctgc agacgtcggg cgtcaacacc
     4621 gtccggccac gcttcgaggt cgacgagtcg ggagccttca cctcgttcgc ggtggagcag
     4681 acggcccatc cggagttccc gacgttgcgt cggcatcggc tcgctgtcgg tctctacgac
     4741 ctcactgatg cagggctggt ccggcgtacg tcggtcgaga ccgatgtgtc cggtgcggag
     4801 acgtacgtcg ccgagctcgt gggtgaacgg cagcctgacc tggtgctgct caacgacggc
     4861 gacctcacgt atgcgaaggt gcggctcgac gagcgttcgc tggccacggt cgtggcgaac
     4921 atcgaccgct tcacccagtc gctgccccgg gctgtgtcgt ggggcgctgc ctgggacatg
     4981 acacgagacg cagagatgcg ggcgtccgac tgggtcgagc tggtgctgcg tggcatccgg
     5041 gtcgagaccg accagttcgc cgtacgttca ctcgtggcgc gcaccggcca ggccatctcg
     5101 cagtacacag cccccgggaa ccggcgggcc gtggccgccc gctgggagac ggggttgctc
     5161 gcgctggtgc gcgacgccga gcccggcagc gaccagcagc tggcattcgt gcgcgggctc
     5221 gcgcaggcgg cgcacaacga cgaggcgctc ggcctggtcg ccggactgct cgccggctcg
     5281 gagactctcg acggactcag cgtcgacaca gacctgcgtt ggcagcttct caacggcctc
     5341 gcccgagagg gacgcgccga cgacgaggcc atcgaccagg agctggaccg ggacatgacg
     5401 atctccggcc aggaggaggc ggcgtcggcc cgggcctcga tgccgacagc ggaggccaag
     5461 gcgcatgcct ggcgggaggc ggtggagaac cgcgagaccg ccaacgagac gatgcgcagc
     5521 atcgccgtcg ccttcaacca gcctggccag gaggaggtgc tgcgcgacta cgtgccgaag
     5581 tacctcacga cagccgagac gatctgggag gagcgggggg tttacgtcgc ctcgacgatc
     5641 ctgagcaaca tgttcccgtc gtcgatcccc acccaggaga ccctcgacac cgtggacgcc
     5701 tggctggcga cgacgaaggc caatccggca gcccggcgtc tcgtgtcgga aggacgcgac
     5761 gacgtcgccc gagcactcgc cgcccagacc cgcgactccc acccctgacc ctcatgccga
     5821 ggtgggggca gctcagggag tgtcggtgtg gagggagtgc tccggcttgg caagtggcgc
     5881 gagctgttga agaccggcga gcaacccccg cgtcaggtcg ccggtcgcga aagcggactg
     5941 catgttgatc gccgccagcg ccgcctggtg gttggtcagc gtccggcgta cctctgaccc
     6001 ggtcacgatc tccaggaccc gggccgctgg gtcgacgtgg atcaacacgc tcgtcgacgg
     6061 gtcgtccagg gcagcgtgca accgctcggc gtactcccgg ggatcgctcg tggaaggtcc
     6121 gacgtggacc gagaacgtcc ggccggacac ccgttcagcg tcggcgatcg ccttggcgat
     6181 ctcgttccgc tgcaccgcgg tgaaggcgtc accagctggc acttgcgccg cctgagtcgt
     6241 cggctcgagg aggctccgac ccgaccttcg acgcctccaa ctcgggtgcc tcggcggctg
     6301 cctcgacagc gctcggggcc ccgaaccact cggcgttcgc atcccactgt tcggtcaacc
     6361 ggtagcgcgg acccttcgcg agggacggcg ccatcacgag caatgtgatc accgcgatga
     6421 tgagcagcgg aatcccgacg aagaacagca cggcgtgcaa cgtcgacatc ggctcggggt
     6481 cgggccacgt ggtgggggtg tcggcgaggg gagtcacgtg gctacgttat cgcctcggcc
     6541 cgtctcggca gcccgagacc ggccaccgct tccagcacgc cggctcttgt acggtcgtgg
     6601 aggcaggcgc tctcccggag cacccctgac tgcggaggac catcgtggct gcatctgatg
     6661 cctgcgacac cggcgacgat ctgtgtcgct acgtcgccaa cctcacgaac agcgaaggcc
     6721 tggcgaaggc atccaactgg ctcatcgcca agcctgccgc gattcttgcc ctcatcctgg
     6781 gtggactcat catccggtgg cttctccacc gggccatcga ccgtctcgcc agacgggccg
     6841 ccgccggaac cgtgccgggc gtgatcgcac gcggcaaggt cccgcagatg ttcctcgagg
     6901 agaacgcggc catcgcggaa cgccgcaagc agcgcgcaga gacgatgggc agccttctca
     6961 agagcatcac cacgggtgtc gtcttgtcga ttgtggcgat catggtgatc gcccagctcg
     7021 gctacaacat cgcgcctctc atcgccagtg ccggcatcat cggcgtcgcg ctcggtttcg
     7081 gggcgcagag tctcgtcaag gacttcatca gcgggatctt catgatcctc gaggaccagt
     7141 acggagtcgg tgactcgatc accgtcggtg aggtcagcgg aaccgtggag gcggtgggac
     7201 tgcgcgtcac ccgcatccgc gacgtcaacg ggacggtgtg gtaccagcgc aacggagaga
     7261 tcctcgccgt aggcaacatg agccagaact gggctcgcac cgtgctcgac atcccggtgg
     7321 cgttctccga ggacctcgcc cggatccgtg agatgctgca cgaggtggcg caggacctgt
     7381 gggaggaccc ggagttccag ggcgacatcc tcgaagaacc cgaagtctgg ggggtcgagc
     7441 ggtgggaccc cgacggcgtc gtcgtacgcg tcgtcttgaa gacggctcca ctcagacagt
     7501 ggaccgttgc ccgtgcgatg cgggagcgga taaaagaccg cttcgatgcc gagggcgtcg
     7561 agatcccgct tccccagcgg atggtctggc accgcgatgc gtcggcacct tcggacaccg
     7621 ctgtggccga cgctccctag tcggcgagag cggcgtcgag cttgatcgtc gtaccggtca
     7681 atgcctgcga gacggggcag ttcaccttgg cgtcctccgc ggcagcgacg aactcgtcgt
     7741 tgctgagccc ttcgacgcgg gcgcggaccg tcagccggat ctcggtgata cccgtgccgg
     7801 cgacgaagtc gacatcggcc ttcgtgtcga cctgctgcgg cgggtgcccg ttgcctgcga
     7861 ggccatgcga cagcgccatg ctgaagcagg tcgagtgcgc ggccgcgatg agctcctccg
     7921 gactcgtccg tccgtccggc gcttcggcgc gcgaggccca ggtgacgtcg aacgagccca
     7981 ggttcgacga gtccagcgag acgttgcccg atccctcgat caatgagcct tcccagtggg
     8041 cgttggcggt gcgcgtggtg gccattcgaa acctcctgct caacgaacgt ggacgtctgc
     8101 catcctgccg tgttgcgggc cactctgctt ccacaggtcg agctgttgcg agaatggcaa
     8161 cgtgaccgcc cgggaagagc acgtcgagca gaccgagcag tccttctacg acgccgtcgg
     8221 cggacacgag accttcgtcc gcctggtcga ccgcttctac gccggtgtcg ccgaggatcc
     8281 cgtcctccgg tcgctctacc cggaggccga cctgggcccc gcgacgaccc ggctccggat
     8341 gttcctcgag cagtactggg gcggtccgac gacgtactcc gacacgcgcg gccatccacg
     8401 gctcggcatg cggcacgcgc cgttcgcggt gactccgacc gcacgcgacc actggcttgc
     8461 gcacatgcgg gtagcggtcg aggaatccgg tatgtcgccg gagcaccagg atcagttctg
     8521 gcagtacatc gtgcgcgccg cgcacttcct ggtgaacacc ttcgacgaag aaccgccgac
     8581 gacgagcggc cggtccctcc tctgacggac gtccgcgcgt cagccttgcg tcgcgtcccg
     8641 catgaagcga ttcagtacgt cgcgctcgat ctcggtcagg cgccgcggtg caccggtctt
     8701 catgtccacc ggcgccagga ccgtggctgc acgaccgtag aggatgcgct tctcgtccgg
     8761 tcccggctca gcgatctcgt agctgatcgt gaatgacgcc ccgcccacct tcgacaccca
     8821 cagctcgatc ggatacggct cctggcggaa cagcatcgct cgccggtagt cgatctccgc
     8881 acggacgacc agcacgccct ccgcgagtcc ctcggctccc cgcgccggcg cgtggacgaa
     8941 cagcatgtcg acccgggcct cctggaggta ctccagccaa gtgacgttgt tgacgtgacc
     9001 gtacgcgtcg aggtcggacc acctgggctt gaggttgaac acgtgtcggg tcacggttca
     9061 tctttgcaaa gtcccgctcg gcaagggctc gagcgaacga cccaaggatg ccgtctaggc
     9121 ttcgacgtgt agccgtccga gccgccgctg agaccggaga gtcccatgcc cgaagctgtc
     9181 atcgtcgcca ccgcacgctc cccgatcggt cgggccggca agggctcgct ggtcgacgta
     9241 cgacccgacg atctcaccgc cgccatggtc gctgctgcac ttgcgaagat tccgcagctc
     9301 gacgcgcacg acatcgacga cctgatgctc ggctgcggcc tgccgggtgg cgagcagggc
     9361 ttcaacatgg cccgcgtcgt ggcgatcctg ctcggctacg accaacttcc cggtacgacg
     9421 ctcacccgct actgcgcgtc gagcgtgcag acctcccgga tggcgttcca cgcgatcaag
     9481 gccggcgagg gggaggtctt cgtgtctgcc ggagtcgaga cggtctcgag gtacgtcaac
     9541 gggagctccg acagccatcc cgggacgcag aaccctgtct tcgacgacgc ccgcgcccgg
     9601 accagggccg cggccgaagg gggcgcgcaa ccatggcacg acccgcgggc ggacggcatt
     9661 cttccggaca tctacatgcc gatgggccag accgccgaga acctcgcaca gtcgcgcggc
     9721 atcagccgcc aggagctcga cgagttcggc gtacgcagcc agaacctcgc cgagaaggcg
     9781 atcgccggcg gcttctggga gcgcgagatc gtcccctaca cgacgccgga cgggaccgtc
     9841 gtcgacaagg acgacggtc
//
LOCUS       DATBFZ010000311         2503 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_1522498, whole genome shotgun sequence.
ACCESSION   DATBFZ010000311 DATBFZ010000000
VERSION     DATBFZ010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1522498"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..558)
                     /locus_tag="VH419_09515"
     CDS             complement(<1..558)
                     /locus_tag="VH419_09515"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY7043890.1"
                     /translation="MLLILGAPMAAAEFAALAHALAGDHTVVTADPRGIAGSRLDDPD
                     QDSTPELRADDVAAILDDLGAASADMFGSSGGAVTGLALLARHPGRVGTLVAHEPPLL
                     ELLPDAAEQRAATEDIIATFSRDGMFAAWGKFMANAGFDVPAGAPEMPAPSDQELRDA
                     AHFFNHELRATTRYLPDVEALKNGRV"
     gene            complement(688..1962)
                     /locus_tag="VH419_09520"
     CDS             complement(688..1962)
                     /locus_tag="VH419_09520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein"
                     /protein_id="HEY7043891.1"
                     /translation="MAADIVPVRLGLTKGDLYTLWAPRWRDAGDEWEAFLGEGDALYA
                     FESVADLVAFVRTNTDNDLADHPSWGKLTAANAHKLQPSEDREYDIVGVPDLVAEKPT
                     EDSVASLHRTLVVVSSIGSVCELAAISKFFNGNPVLGTLGGGLEGFTGRSGRKRWSEI
                     EAVIARGWDGVLDSIDEIVSIPEDIDADAVKKAEAQLEEPAPEEDEDDLAVDADSEDS
                     EDGDEDDVAAEPVRSAADSAVLGDDVDFWQKVGIDPVRIMTGSGTVYTLRCYLDDDPV
                     FLGRNGRISVFSSERALARYLADEHDHDLSDLATYDDIRTAATDGSLRVDVAEENVYV
                     LSGISDDIADGPDAVDHDQLELAVELLRDVSDYSEDKTVDETLAGTKALGKFVSYVLG
                     DEGARKPEAPYAQAVEQWDALERFVESRLRAE"
     gene            2089..>2503
                     /locus_tag="VH419_09525"
     CDS             2089..>2503
                     /locus_tag="VH419_09525"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY7043892.1"
                     /translation="MADVGDRQPTAAGLSCGACGAQVAATAKFCSQCGAPIARTAQLA
                     EYKQVTVLFADVVHSMDIAAAVGAERLREIMTELVARSAAVVRRYGGTVGSFTGDGIM
                     AVFGAPLALEHHAVHACLAALDIQDEAKRLGPEIHR"
BASE COUNT          376 a          826 c          887 g          414 t
ORIGIN      
        1 cacccgcccg ttcttcagcg cctcgacatc gggcaggtac cgcgtggtgg cccgcagttc
       61 gtggttgaag aagtgtgccg cgtcgcgcaa ttcctgatcg ctcggcgcgg gcatctcggg
      121 tgcaccggca ggcacgtcga agccggcgtt ggccatgaac ttgccccagg ccgcgaacat
      181 cccgtcgcgg gagaaggtgg cgatgatgtc ctcggttgcg gcgcgctgct cggccgcgtc
      241 gggcaacaac tcgagcaggg gaggttcgtg ggccacgagg gtgcccactc ggccggggtg
      301 ccgggccagc agggccagcc ctgtcaccgc gccgcccgac gagccgaaca tatcggccga
      361 ggcggcgccc aggtcgtcga ggatcgccgc gacgtcatct gcccgcagtt cgggcgtcga
      421 gtcctggtcg ggatcgtcga gacgactgcc cgcgatgcca cgtgggtcgg cggtcaccac
      481 ggtgtgatca ccggccagtg cgtgcgcaag cgcggcgaac tcggctgccg ccatcggggc
      541 gccgaggatc agcagcaacg gtccctgccc gcgcacctca tagtgcaggc gggcgccggg
      601 aaccgtgacg gtgtgggtgg agaggttgct cgaggtggtc atgagtggtc tgaccgcttt
      661 cgcggccagg actcatcgcc ggacgggcta ctccgcgcgc aggcgcgact ccacgaaacg
      721 ctccaacgca tcccactgct cgacggcctg ggcgtacggg gcctcgggct tgcgggcgcc
      781 ctcgtcgccg agcacgtaac tcacgaactt gccgagcgcc ttggtgccgg ccagcgtctc
      841 gtcgacggtc ttgtcctcgg agtagtcgct gacgtcgcgg agcagttcga cggccaactc
      901 caactggtcg tggtccaccg cgtcgggtcc gtcggcgatg tcgtcggaga tgccactgag
      961 gacgtagacg ttctcctcgg cgacgtcgac ccgcagggag ccgtcggtgg cggcggtcct
     1021 gatgtcgtcg taggtggcca ggtccgacag gtcgtggtcg tgttcgtcgg ccaggtagcg
     1081 ggccagcgcg cgctctgagc tgaacacgct gatgcgaccg ttgcggccga ggaagaccgg
     1141 atcgtcgtcg aggtagcagc gcagggtgta gacggtgccc gagccggtca tgatgcgcac
     1201 cgggtcgata ccgaccttct gccagaagtc gacgtcgtca cccagcacgg cggagtcggc
     1261 agcactgcgc acgggttcgg cggccacgtc gtcctcgtcg ccgtcctcgg aatcttcgga
     1321 gtcggcgtcg acggcgaggt cgtcctcgtc ctcctcgggt gccggttcct ccagctgggc
     1381 ctcggccttc ttgaccgcgt cggcgtcgat gtcttcaggg atgctgacga tctcgtcgat
     1441 ggagtcgagc acgccgtccc agccccgggc gatgacggct tcgatctcgg accaacgctt
     1501 gcgcccggac cgaccggtga aaccttccag cccgccgccc agagtgccga gcacggggtt
     1561 gccgttgaag aacttgctga tggcggccag ctcacacacc gagccgatcg acgacaccac
     1621 caccagggtg cggtgcaggc tcgcgaccga gtcctcggtc ggcttctcgg cgaccaggtc
     1681 gggcaccccg acgatgtcgt actcgcgatc ctccgacggc tgcagcttgt gcgcgttggc
     1741 agcggtcagc ttgccccacg acgggtgatc ggccaggtcg ttgtcggtgt tggtgcggac
     1801 gaacgccacc aggtcggcga ccgattcgaa ggcgtacagg gcgtcacctt cgcccaagaa
     1861 ggcctcccac tcgtcgccgg cgtcccgcca acgcggagcc cacagtgtgt aaaggtcgcc
     1921 cttggtcagc ccgagccgga ccggcacgat gtcagcagcc atgcggcaca gcctaatggg
     1981 gtgccctgga cgccagtcag gttgtctccg gttcggggcc cggatcggcg tcgccgggcc
     2041 gacatcgaat ctgcggacac ggctgacctg cggtgaaaca atatcggcgt ggccgacgtc
     2101 ggcgaccgcc aaccgacagc tgccggtctc tcctgcgggg catgcggcgc tcaggtagct
     2161 gcgaccgcca agttctgcag tcagtgcggc gcgccgatcg cacgcaccgc gcagttggcg
     2221 gagtacaagc aggtcacggt gttgttcgcc gacgtcgtgc attcgatgga catcgccgcc
     2281 gcggtgggcg ccgaacggct gcgcgagatc atgaccgagt tggtggcgag gtcagcggcg
     2341 gtggtgcgtc gctacggcgg caccgtcggg agtttcaccg gcgacggaat catggcggtg
     2401 ttcggcgcgc cactggcatt ggagcatcac gccgtccacg cgtgtctggc ggcgttggat
     2461 attcaggacg aagcgaaacg gctcggaccc gagattcacc ggc
//
LOCUS       DATBFZ010000312         2120 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_5032207, whole genome shotgun sequence.
ACCESSION   DATBFZ010000312 DATBFZ010000000
VERSION     DATBFZ010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5032207"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..532)
                     /locus_tag="VH419_09530"
     CDS             complement(<1..532)
                     /locus_tag="VH419_09530"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY7043893.1"
                     /translation="MDDLYQGAHDVHVLPANLSLPGMGVLPVNAFVLMAEEPVLIDSG
                     IGVDGDDFVAAAGSVIDLERLKWVWLTHDDADHTGSIQRIMEAAPNATLVTHALSALR
                     MSTWWPVPMERVHAIRPGDEIHVGDRSLVAVPPPLFDNPTTLGVLDRSTGCLFTADAF
                     GAILPEATQHVSEVPRE"
     gene            846..1295
                     /locus_tag="VH419_09535"
     CDS             846..1295
                     /locus_tag="VH419_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020388426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043894.1"
                     /translation="MAAKSAKTQSEGFSAEERAAMKERAAELRAEGKKGAKKADGLQA
                     VLDSIEKMAPQDRALAERVHVTVTTTAPDLSPKTWYGMPAYANADDKVVVFFQDSGKF
                     KYRYSTLGFQDSANLDDGDVWPTSYALQNWSPAVEKKVVELVKAAVS"
     gene            1341..1724
                     /locus_tag="VH419_09540"
     CDS             1341..1724
                     /locus_tag="VH419_09540"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEY7043895.1"
                     /translation="MPGDPNTPDAFDVYTVVVLRQPADAPELSDEELDALQARHLAYR
                     AELRRQGLLAANGPFDEQSDPSYRGMSIFACDAAEAARLSDEDPSVVAGRLSYDVMEW
                     WVRAGSLAFPLAGSPIGDRRSMPDD"
BASE COUNT          357 a          704 c          717 g          342 t
ORIGIN      
        1 cctcgcgagg cacctcggac acgtgctggg tggcctcggg gaggatcgcg ccgaaagcgt
       61 ctgcggtgaa caggcagccc gtcgaccgat cgagcacgcc gagcgtcgtc ggattgtcga
      121 agagcggcgg cggcacagcg acgagcgagc ggtcaccgac gtggatctcg tctcccgggc
      181 ggatggcgtg cacccgttcc atggggaccg gccaccacgt cgacatcctg agtgcgctca
      241 gcgcgtgcgt gacgagcgtg gcgttcgggg cggcttccat gatgcgctgg atgctgccgg
      301 tgtggtcagc gtcgtcgtgc gtcagccaga cccacttcag gcgctcgagg tcgatgaccg
      361 agccggccgc cgcaacgaag tcgtcgccat cgacgccgat tcctgagtcg atgagcacgg
      421 gttcttcggc catcagcacg aaggcgttga ccggcagaac acccatcccc ggcagtgaca
      481 ggttcgcggg gaggacgtga acgtcgtgag ccccctgata cagatcgtcc atgagcttgc
      541 ctccgcggtc ggtatctccg gatccgtccg acatccccgg ctcgagatgg tcggacgact
      601 gacacgagtc ccccgctgcc ccggcccccg ggaggcagac gccgccagtg tgctcgcgac
      661 gccatgtgcc atccatggca cgttcgggtg gttcccagtc ggcgagccgg tccacggtgc
      721 tccgtgtctc ggcaggatcc cgattcggtc aggaatcgag aagcgccagg gtcacgtcgg
      781 ggcctagcct tccttcagac ggccggcatc acgccgggca ccgagcgtcg aaaggcaatc
      841 ccagaatggc tgcgaagagt gcaaagaccc agtccgaagg cttctctgcc gaggaacgtg
      901 cagcgatgaa ggagcgcgcc gctgagctgc gcgccgaggg caagaaaggc gcgaagaaag
      961 ccgacggact tcaggcggtc ctcgacagta tcgagaagat ggcgccgcag gatcgtgcgc
     1021 tcgccgaacg cgtgcacgtg acggtgacca cgacggcacc agatctttcg ccgaagacct
     1081 ggtacgggat gcctgcctac gcgaacgcag acgacaaggt cgtcgtcttc ttccaggact
     1141 cgggcaagtt caagtatcgg tactcgaccc tgggcttcca ggactcggcc aacctcgacg
     1201 acggcgatgt gtggccaacc tcgtacgcac tccagaactg gagccccgcg gtggagaaga
     1261 aggtcgtcga gctcgtgaag gctgcggtct cctgacggtg acgccagacc gaactggacc
     1321 gcgctgtcga ggagggcacc gtgccaggtg atcccaacac tcccgacgcg ttcgacgtct
     1381 acacggtcgt cgtcctacgg cagccggcgg acgccccgga gctgtcggac gaagagctcg
     1441 acgcgctgca ggcacgccac ctcgcctacc gcgccgagct gaggcgacag ggcctgctgg
     1501 ctgcgaacgg gccgttcgac gagcagagcg acccgtcgta ccgcggcatg tcgatcttcg
     1561 cctgcgacgc cgccgaggcc gcacggctgt cggacgagga cccgtcagtc gtcgccgggc
     1621 ggctcagcta tgacgtgatg gagtggtggg tccgtgccgg ctcgctggcc ttcccgctgg
     1681 ccggcagccc catcggcgat cgccgctcga tgcccgacga ctgaagcatc acgaccgatc
     1741 accatctgtt catggtgccc tgatcggagt ctgcggaagc cgtggagtgg ggcaatccgc
     1801 tggtggatcc gggtcgagcc ggtcatcatg ggtcgcatgt ctgctccggt cgctgccgct
     1861 cactgaatca cgcgtttcct cgcctgatga cgctcgtcgc cgatccgacg ggccctagag
     1921 tgcagccccc agcttggagc cattgccttg accatccagt cgtcgtgggt ccagatcgcg
     1981 tgtgacgagt ccgggttctc cgggagcaac ctcctcgacc cggcctcgcc ggtgatcacg
     2041 cacgccagcg tcgatctcgc cgtaccggag gccgccgagg tggtggccgt actccggtcc
     2101 cgtcgtggcc ggcgttcgga
//
LOCUS       DATBFZ010000313        22345 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_870120, whole genome shotgun sequence.
ACCESSION   DATBFZ010000313 DATBFZ010000000
VERSION     DATBFZ010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 22345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22345
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_870120"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(86..361)
                     /locus_tag="VH419_09545"
     CDS             complement(86..361)
                     /locus_tag="VH419_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HEY7043896.1"
                     /translation="MADRIDLVLRSFFTPDGRLHTMPAKRGKRLVVLDHIAQSFEPGR
                     TYPERDVNQLLERFHTDHASLRRFLVDEDFLTREQNIYWRSGGTVDL"
     gene            complement(412..1326)
                     /locus_tag="VH419_09550"
     CDS             complement(412..1326)
                     /locus_tag="VH419_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007078391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY7043897.1"
                     /translation="MQHSIEEMADLGNGITVCYDTFGKPNDPALLLIMGLAGPLNWWN
                     PDLCALLAERGFYVVRFDNRDVGKSTRLDNLRARRIDVVRAFVRRRAVPPYTLSDMAD
                     DAIGLLDHLGIRKAHVTGVSMGGMIAQTLATEHQDRVLSLVSIMSTTGRWSVGWQDPR
                     LFPRLLGRGRNEREDYIRQSAKTWTAIGSPGYPTPPDEVRERAGETYDRGVSMTGAIR
                     QMEAVLAQPDRSRALHDLRVPTMVIHGLSDRLVHVSGGRATARAIPGAELVLVPGMGH
                     DLPKPLWPRFADGIERTARRAQTEAVHR"
     gene            complement(1498..2460)
                     /gene="glpX"
                     /locus_tag="VH419_09555"
     CDS             complement(1498..2460)
                     /gene="glpX"
                     /locus_tag="VH419_09555"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012923147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HEY7043898.1"
                     /translation="MELVRVTEAAAMAAGRWVGRGDKDGADGVAVNAMRALIGTVGMD
                     GIVVIGEGEKDNAPMLYNGERVGDGTGPPCDVAVDPIDGTTLAAKGMSNAVAVLAVSP
                     RGSMYDPSAVFYMEKLATGPEAAEVVDIRLPVAENIKRTAKAKGSAKEDITVVVLDRP
                     RHEGLVDEIRETGARIKFITDGDVAGAIMAGRPDTGIDLLLGIGGTPEGIIAACAMKA
                     LGGVLQGRLWPREDDERQKAIDAGHDLDRVLTTDELVSADDCFFVATGITDGELLRGV
                     RYAHGGAETHSLVMRSRSGTIRSIRSEHRFEKLRAYAAVDFEHA"
     gene            complement(2613..3677)
                     /locus_tag="VH419_09560"
     CDS             complement(2613..3677)
                     /locus_tag="VH419_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020391581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY7043899.1"
                     /translation="MSLVADFVVRRPAHTTTIDLVAQAGEVVGLIGPNGAGKSTTLRA
                     LAGLIRVDDGAIELDGVTLSSPDVHIPPQERSVGYVFQDHLLFPHLSAVDNVAFGPRS
                     RGIRRRPARQRAQEWLEHLSIADLADRRPRQLSGGQAQRVAIARALAANPGMLLLDEP
                     TASLDAAGAMTLRTQLRDHLHEFAGVCLVVTHTALDAMVMADRLVVLDEGRIVQSGTP
                     SEVAARPRTHHVAALVGLNLVRGEADRGVIRLDETTAVVAGEALGGAAFAAFSPAAVS
                     LFVDEPAGSPRNVWRGSVTSLAPHGDSVRIQIDAALPLLADVTPAAVATLDLRPGRNV
                     WASVKATEVSIYPAESSGSG"
     gene            complement(3674..4483)
                     /locus_tag="VH419_09565"
     CDS             complement(3674..4483)
                     /locus_tag="VH419_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012924045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY7043900.1"
                     /translation="MNRSGIAIRGRPPLGLIVPAVMALAFLVVPVVAVLVRAPWGTLA
                     DELGTQAARDALRLSLQTTLTATFACIVLGVPLAWVLARTTFRGRSFVRALVTLPLVL
                     PPVVGGVALLMAFGRNGIVGRWLDEAFGITLPFTTTAVVMAQTFVALPFLVISVEGAF
                     RASDERYDDAAATLGASRWSTFRRVTLPLAMPGIVAGAVLCWARALGEFGATVTFAGN
                     FPGTTRTMPVQIYLALQESPDGAIALSLVLIVVSVAVLALLRERWLSGAAR"
     gene            complement(4480..5262)
                     /gene="modA"
                     /locus_tag="VH419_09570"
     CDS             complement(4480..5262)
                     /gene="modA"
                     /locus_tag="VH419_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017934502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY7043901.1"
                     /translation="MKVIRPIHRRTIGAGSAALLLAACLVSCSDGDGGGGDTTQLTVF
                     AAASLTEAFTTLEKDFEKDHSDVDVVTSFGSSTDLATQVTEGSPADVIATADDKSMGI
                     VTDAGDLDGEPTQFATNTLIVVTPPDNPGNVRSLGDLQHADYVVCDPSVPCGAAAATI
                     LDNAGITAKPKSLEEDVKAVLTKVTLGEADAGIVYVTDAQAAGDKVSTVDIAPDVNVV
                     NPYFIGVIKDSGESHLAQEWVDLVTSQAGQSVLQQDGFGAAP"
     gene            complement(5259..5663)
                     /locus_tag="VH419_09575"
     CDS             complement(5259..5663)
                     /locus_tag="VH419_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008358984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HEY7043902.1"
                     /translation="MTVYRLAEAATLLGVSDDTVRRWSEAGRFPRARTADGHAGIEGI
                     ALARLARQLADLPIEGTAGGSHRVSARNQLRGIVTEVKRDSVMAQVELCCGPYRVVSL
                     MSREAADALRLEPGVLAVASIKATNVVVEVPT"
     gene            5898..6428
                     /locus_tag="VH419_09580"
     CDS             5898..6428
                     /locus_tag="VH419_09580"
                     /inference="COORDINATES: protein motif:HMM:NF025396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4245 domain-containing protein"
                     /protein_id="HEY7043903.1"
                     /translation="MSQTRSGYRTSVAGLVGALLVTLPLLAGVYLFTLLGRHEAPDPV
                     TPVDYTAELATARAEAPFAVLAPESLPAGWRATSVNWDGAGPENAWHLGFLTDTDEYV
                     GLEQGNAVAQEFVADKTPADQPGEPVRIEGTIWQTLTGNGDTALVHVEDGVTTIVTGT
                     APESTLVAFARSLRPS"
     gene            complement(6991..7251)
                     /locus_tag="VH419_09585"
     CDS             complement(6991..7251)
                     /locus_tag="VH419_09585"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002549311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEY7043904.1"
                     /translation="MTDDQASDQSSDELSYEAARDELVSIVQQLEAGGTTLEESIALW
                     ERGEQLAKICQRWLDGARARLDAVLAETSAAHDAPDGGQQGS"
     gene            7317..7718
                     /locus_tag="VH419_09590"
     CDS             7317..7718
                     /locus_tag="VH419_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020575635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY7043905.1"
                     /translation="MIEDMPTSLITQLTPRLVVAEAAKAIDFYCTALGADEQERFEVD
                     GAIAHARVTIDGLDVALKDADEFDLAPPSLGGTPVIISLTVTDADAVGQRMVDAGATV
                     VFEVGDRDYGFRDGRLRDPFGHLWIVSQPLR"
     gene            complement(7725..8945)
                     /gene="xseA"
                     /locus_tag="VH419_09595"
     CDS             complement(7725..8945)
                     /gene="xseA"
                     /locus_tag="VH419_09595"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEY7043906.1"
                     /translation="MALETTQDKPAPVRVIANAMTGYINRLGPVWVEGQIAQLTRRPS
                     TVFLTLRDPLAEVSVQVTCNRAVLDAMPTPVTEGARVVVHAKFTYYVPRGVLSMVARE
                     IRPVGIGELLARIEQRRQLLAAEGLFDTARKRPLPFLPRMIGLVVGRDSAAERDIVDN
                     ARRRWPGVSFRTEYAAVQGYDAARQVIEALRVLDKEQEVDVIIVSRGGGALEDLLPFS
                     DEGLIRAVAACTTPVVSAIGHEEDQPLLDLVADLRASTPTDGAKSVVPDVAEEAAKLV
                     TVRDRGRFVLGSMLRRELHALETTRSRPVLAQPHRVLDRFSEQVDVLLARSRRTFDHH
                     LARAEEDLAHRVARVRALSPLATLQRGYAVVQTTDGHVVRAVSDVGPGTTIDARLADG
                     RLLGTVAEVYPDDS"
     gene            9102..10136
                     /locus_tag="VH419_09600"
     CDS             9102..10136
                     /locus_tag="VH419_09600"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020580289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEY7043907.1"
                     /translation="MSSVASGSSSTPSRASSTWSTPQPDGRQVLLAAPRGYCAGVDRA
                     VITVEKALDVYGPPIYVRKQIVHNKHVVRTLENRGAIFVEELDEVPEGSTVVFSAHGV
                     SPAVHEQASERRLKTIDATCPLVTKVHNEARRFANDGYDILLIGHEGHEEVEGTSGEA
                     PDNITLVQGPGDVADLDLRDPQRVAWLSQTTLSVDETMQTVDRIRQRFPNLLDPPSDD
                     ICYATQNRQQAVKEIANGADLVIVVGSGNSSNSVRLVEVALEAGAQAAYRVDDASEID
                     EEWLEGADSVGVTSGASVPEDLVEGVLDFLGERGYPMASSVHTAEESLIFALPPELRR
                     DLREAQPAND"
     gene            complement(10161..10748)
                     /locus_tag="VH419_09605"
     CDS             complement(10161..10748)
                     /locus_tag="VH419_09605"
                     /inference="COORDINATES: protein motif:HMM:NF042451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6542 domain-containing protein"
                     /protein_id="HEY7043908.1"
                     /translation="MASSPHPAPDVLASEADVALLGTLEPEAEDVDLPDQGYGEAPYS
                     PSSASAYVDDSYEPEPVESGRRATVYESLPGMPGRGVVLMAGLATAGTAVLDFALTGR
                     LSFFFDLTFVVICLVTAMAVRGHDLFTTGVLPPLLFGATVAVVTIAAPETIIAAGNLS
                     KTFLTGLTSHAGALAFGYGTALVTVAGRTLEHRRR"
     gene            complement(10866..12065)
                     /locus_tag="VH419_09610"
     CDS             complement(10866..12065)
                     /locus_tag="VH419_09610"
                     /inference="COORDINATES: protein motif:HMM:NF014680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEY7043909.1"
                     /translation="MNATTFVIAGLTGAAGLAIGVVVGLWLAARSRSDLHEQLRLLSA
                     QAVSESTNQVLALADSRVRATEQVVQPVRESLDRLNDRIGTLEQQGASWQSQLKQQVE
                     SVHLSGIELRQQTAALAEALRRPHVRGTWGELQLRRSLELAGLSAHCAFDEQVTRQTD
                     DGRLRPDVVVQLTGGKQIVVDAKVPLDAFLSATEVQGARRDAELQRHARQVRAHIDAL
                     AGKAYWLQFSPAPEFVVMFLPAEAIFAQALDTDPTLIEYAAGKGVVLATPTTLIAMLK
                     TVSYAWTQEALADNAREVHALGRELYDRLGTVGSHLDKLGRSISAVVGSYNQAVASME
                     TRVLVSARRMRDLQGIEKSLPETPAVTDLPRPLTAAELLDRPEPASPAIEGDPGREQF
                     SRHAVGE"
     gene            12199..13116
                     /locus_tag="VH419_09615"
     CDS             12199..13116
                     /locus_tag="VH419_09615"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020386433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEY7043910.1"
                     /translation="MTDYRAVVDAEISFANGGGISVSGFRLDIPGQDTGRDEIGQLLV
                     RSLGLLMADDVRMGSIEIIAEQHKGTRGGPADVSAGRTGRGRGRLVDLSHPIRAGMVT
                     LPGLPGPEITRHLTREASKAIYAPGTTFEIGRISMVANTGTYVDAPHHRFAGGVDLSG
                     LELSSLADLPTIVVRLRDTPPGGIGVEALAAYDVKGHAVLLETGDSDRFGDPEYVENP
                     SFLTRDGASWLVAHGVQLVGIDAANIDDMGDGTRPAHTLLLQAGIPVVEHVTGLSEVP
                     ATGARFFAVPPKVADFGTFPVRAFALIPA"
     gene            13208..13372
                     /locus_tag="VH419_09620"
     CDS             13208..13372
                     /locus_tag="VH419_09620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043911.1"
                     /translation="MTGDERAGGIVDVGRVTERGDRAVEGASGGGDRRAGTDRTPADE
                     PTRRLTRTTQ"
     gene            13372..13914
                     /locus_tag="VH419_09625"
     CDS             13372..13914
                     /locus_tag="VH419_09625"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY7043912.1"
                     /translation="METERTRLRGWRADDVDRVLDIYSRWEVSKWLGAEPKVMQTREQ
                     AEQFVERVIARELRRDATGFWAVERKADQVVAGTLLLMPLQGGERDEVEIGWHFHPDS
                     WGQGLASESARALLTWGFEHGLDEILAVVRPGNAASMAVCRRIGMTHLGRTSTYYATE
                     LELFRINRPSTAEVATFVHI"
     gene            14014..15090
                     /gene="ychF"
                     /locus_tag="VH419_09630"
     CDS             14014..15090
                     /gene="ychF"
                     /locus_tag="VH419_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008358950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEY7043913.1"
                     /translation="MALTIGIVGLPNAGKSTLFNALTKNDVLAANYPFATIEPNVGVV
                     GVPDERLGILAKLFESARQVPATVQFVDIAGIVRGASEGEGLGNKFLSHIRESDAICQ
                     VTRVFRDDDVTHVEGKVSPADDIETIKTELILADLQTVEKAVPRLEKEARLRKEMQPQ
                     LDAVREAQAALSAGTPIIATSIDKSHLRELMLLTAKPYLYVFNCDADELADEELKAEM
                     RAVVAPSEAIFLDAKFEAELVELGDDDEAREMLAEMGIDEPGLDTLARVGFDTLGLQT
                     YLTAGPKESRAWTIKKGATAPEAAGVIHTDFQRGFIKAEIVSYDDLVAAGSMNAARSA
                     GKVRMEGKDYVMQDGDVVEFRFNV"
     gene            15251..15520
                     /locus_tag="VH419_09635"
     CDS             15251..15520
                     /locus_tag="VH419_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020391372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043914.1"
                     /translation="MSPWEPVPSPAPGDEERDVVRMDVDGEEFDVSERPDRTGEYHFD
                     WVSGPNAGYGFTTATSDHSELDQAELEEAIREFLAMVDPETGYIE"
     gene            15605..16150
                     /locus_tag="VH419_09640"
     CDS             15605..16150
                     /locus_tag="VH419_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020385408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEY7043915.1"
                     /translation="MTTPIEPQTIERAEPPFAADERTMLDAWLDFHRATLLTKCEGLT
                     EDQLRTRAVPPSSLSLLGLVRHMADVERSWFRRALAGEARDDAPPQYYSDDNPDGEFD
                     DVDTADALADLARYRDEVARCRQVAAVHTDLEALGNGVRDGRRAPMSLRWIYLHMIEE
                     YARHNGHADFLRERIDGVTGV"
     gene            16273..16668
                     /locus_tag="VH419_09645"
     CDS             16273..16668
                     /locus_tag="VH419_09645"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEY7043916.1"
                     /translation="MQYMSFVKMTGDGGEPPKELQDAMGEEIERGFADGTLVHIGGLY
                     ATSYRTEFRVRAGQVTSTDGPFSEAKEEVGGFAIIDVRDHAEAVENARRLAELHHKLW
                     PGWEGSVEVRRIAGADDIASSGDDPAASV"
     gene            16759..18060
                     /locus_tag="VH419_09650"
     CDS             16759..18060
                     /locus_tag="VH419_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY7043917.1"
                     /translation="MGSAAVTAVWRVESTRLVAALTRITGDVGLAEDVVQDALVAALD
                     QWPRDGVPDNPAAWLMTTAKRRAIDHFRRSESLRRRTAELGHGLEEVEVPDLDAAVDF
                     IEDDVIRLIFLCCHPSLTADSRAALTLRLVVGLSTAEIARGFLVAEATMGQRITRAKR
                     TLASSRARLELPTGADRDRRLDDVMAVIYLIFNEGYAATAGAEWTRPDLCHEAIRLAR
                     VLAGLVPDEPEVHSLQALLELQGSRLHARVDGEGQPVLLADQDRRRWDLLLVRRGQAA
                     LARAERLAEKGRPVGRYYLQAAIAAEHARALRGDDTDWATIAQLYDVLAEAAPGPVVE
                     VNRAVAHGRAFGPEAGLAVLDAIPADELEGSHLPAAVRGDLLVRLGRRSEAAAAFRTA
                     AALTRNQGERILLSRRATDADDMTTPRWQVSLDSAGVSDRD"
     gene            18197..19222
                     /locus_tag="VH419_09655"
     CDS             18197..19222
                     /locus_tag="VH419_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017933587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone reductase"
                     /protein_id="HEY7043918.1"
                     /translation="MRAIEYAVTGGPEVLMLVDREIPEPGPGEVRVQIHRSGVNPTDW
                     KSRRGERAGSKVDPPQVPNQDGSGVVDAVGQGVEAALRGLRVWIWEAAYQRPSGGTAQ
                     EYAVVPARHVVLLPDTASFDVGASLGVPFLTAHRCLTVTEDGPTRLGPGTMQGRTVLV
                     AGGAGAVGNAAIQLARWSDATVITTVSSPEKAHLAARAGADHVIDYTRQDVATEVRAI
                     APDGVHTIVEVSPAVNASLDAQILARHGSVAVYANNGGNEVQLPVRTSMVPNARWQFV
                     LLYTAPDSWRERGLEDLAAAVLDGAARAGEQAGLPLHHFPLEEAAAAHRAVEDGAVGK
                     VLIDVRP"
     gene            complement(19219..20592)
                     /locus_tag="VH419_09660"
     CDS             complement(19219..20592)
                     /locus_tag="VH419_09660"
                     /inference="COORDINATES: protein motif:HMM:NF016693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY7043919.1"
                     /translation="MRTIVIGAGPTGLFTAIALARRGYDVTVVDRDSGPAADGSWDRK
                     GVMQFHHPHGFRQQVVDALRAEMPDVWVRLLEAGAVPATRPEQPDMFVGVRVRRLVYE
                     RALRAAAENEPGVHLQVGNVERIYGRRGRVTGVRVDGDLLGADLVIDASGRSGRLGDE
                     FRAKGEGGDSGISYVSRQYELLPGARPGPMNAPIGAIAMYAGYQAMVFTQDNRTFSTL
                     IVRASTDRDLALLRDTAAFDAAARAIPILAAWTDPDRARPITPVLPGGRLFNTYRGQL
                     DEHGETALSGLLFVGDAVSTTNPALGRGVALSMLQVQELLRLLDTGDGDLRAVSREFD
                     RWCADHIKPWFDDHARWDADQIRRWSGGDVDVSRPLPSDLIIQAAEADPSLMRVVGPY
                     LAMLVPPSALDTIEPTARELYAGGWRPTPPEGPTRDELAAIVTTATTRPTPRATSLAG
                     PIARVAR"
     gene            20681..21343
                     /locus_tag="VH419_09665"
     CDS             20681..21343
                     /locus_tag="VH419_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY7043920.1"
                     /translation="MSEPVKRNYSSTLRETQASETRRVIVSAATRLFIEQGFGRTTID
                     AIAAAAGVSRKTVFTSAGGKADILKLALDWAVVGDDRPVPLSRRSAVERLKKETDPAT
                     LVEGWIEMGVGISARVAGIYGALVVAAGIDDDARDLLETARAQRLAGARAIVRALDAL
                     GALRPDLSVDEAADIAWTLGDPTLYEDLVVHRRWSQRRFKTWMTDTAARQLLAAAPSC
                     QK"
     gene            complement(21394..>22345)
                     /locus_tag="VH419_09670"
     CDS             complement(21394..>22345)
                     /locus_tag="VH419_09670"
                     /inference="COORDINATES: protein motif:HMM:TIGR03118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR03118 family protein"
                     /protein_id="HEY7043921.1"
                     /translation="AQVTDPNLVNPWGVSFSSTSPLWVSDNGTDVSTLYTGGVHGGQQ
                     NILSLVVSIPGGAPTGQVVNPTSDFVVSDDSGNSGTARFLFVGESGHLSGWAPNVPPP
                     MSTQAQDAVVTPGAVYKGLALAQTADGPMLYAADFSAGTVDVYNGMFQRVITAGNFED
                     RRLPAGYGPFNVAVLDGKVYVAYAKQDADREDEVAGHGRGRVDVFSTDGTLLKRLSHH
                     GDLNAPWGMTIAPAGFGKLSGDLLVGNFGDGRIHAFDAQSLAPRGALLDTHHRQIRID
                     GLWALTPGNGTEGGTDEVLFTAGPDDESHGLLGTLSLSVS"
BASE COUNT         3582 a         7610 c         7719 g         3434 t
ORIGIN      
        1 tggtaggacc cgccggtcaa ggctgccgag gtcgcgtcgc cggggctccg gaacggggga
       61 acggcgcggc ctcggcgtac accggtcaga gatcgacggt tccgccgctg cgccagtaga
      121 tgttctgctc gcgggtgagg aagtcttcgt cgaccaagaa gcgacgaagc gaggcgtggt
      181 cggtgtggaa acgctcgagc aactggttga catcgcgttc cgggtaggtc cggcccggct
      241 cgaacgactg cgcgatgtgg tcgagcacga caagccgctt gccccgcttt gcgggcatcg
      301 tgtgcagtcg accgtccggg gtgaaaaagc tgcgcagcac caagtcgatg cgatcggcca
      361 tgccgtcgat cctgacacct tgtcggccta gcctcgtcgg cccgtgctgc ctcagcggtg
      421 gaccgcctcg gtctgggcgc ggcgggcggt ccgttcgatg ccgtcggcga acctcggcca
      481 caacggcttc ggcaggtcgt gtcccatgcc aggaacgagg acgagctccg caccggggat
      541 cgcacgggca gttgcccgac caccggacac gtggacgagg cggtctgaca gcccgtggat
      601 caccatggtc ggcacccgca gatcgtgcag cgctctggac cggtcgggct gggcgaggac
      661 cgcctccatc tgacggatcg cccccgtcat gctgacgcct cggtcgtagg tctcgccagc
      721 ccgctccctg acctcgtcag gcggtgtcgg gtagccagga gacccgatgg ccgtccaggt
      781 cttggcggat tgacggatgt agtcctcgcg ctcgttgcga ccgcgaccta gcaggcgggg
      841 gaacagccgg gggtcctgcc accccaccga ccaccgtccc gtcgtcgaca tgatcgagac
      901 cagcgacagc acgcggtcct gatgctcggt cgcgagtgtc tgggcgatca ttccgcccat
      961 cgacactccg gtgacgtgcg ccttccggat gccgaggtgg tcgagcagtc cgatcgcgtc
     1021 gtcggccatg tcactcaacg tgtacggcgg cacggcccgg cggcgtacga acgcccgcac
     1081 gacgtcgatg cggcgggcgc gaaggttgtc cagccgggtc gacttgccca catcgcggtt
     1141 gtcgaagcgg acgacgtaga agccacgctc ggccagcagg gcgcacaggt cagggttcca
     1201 ccagttcagc ggcccggcca gacccatgat gagcagcagg gcggggtcgt tcggtttgcc
     1261 gaaggtgtcg tagcagacgg tgatgccgtt cccgaggtcg gccatctcct cgatgctgtg
     1321 ctgcacgttt cggcgcctcc agtgctcgac cccttcgggt tgttactcgt cagtatagtc
     1381 cgccaaccca gccggccggc cgtgtcggtg ggtctccgcg aattgcggga aaatggtggt
     1441 cgacagcccg ggcgccacct gacctttccc gcaattcgcg gcaaccacca gcgtgggtca
     1501 ggcgtgttcg aagtcgacgg cggcgtacgc gcggagcttc tcgaaccggt gctcgctcct
     1561 gatcgagcgg atcgtgccgc tgcgtgatcg catcacgagc gagtgtgtct cggcgccgcc
     1621 gtgggcgtac cgcacgcccc gcagcagctc accgtcggtg atgccggtgg cgacgaagaa
     1681 acagtcgtct gccgagacga gctcgtcggt cgtcagcact cggtccaggt cgtgaccggc
     1741 gtcgatcgcc ttctgccgtt cgtcgtcctc gcgcggccag agtcggccct ggagtacgcc
     1801 gccgagcgcc ttcatggcac acgccgcgat gatgccctcc ggcgtaccgc cgatgccgag
     1861 gagcaggtcg atgccggtgt ccggcctgcc cgccatgatc gcgccggcga cgtcgccgtc
     1921 ggtgatgaac ttgatgcggg cgcccgtctc ccggatctcg tccaccaggc cttcatggcg
     1981 ggggcggtcg aggacgacga ccgtgatgtc ctccttggcg ctgcccttgg ccttggcagt
     2041 gcgtttgatg ttctcggcga cgggcaaccg gatgtcgacg acctcggcgg cctccgggcc
     2101 ggtcgcgagc ttctccatgt agaaaacggc cgacgggtcg tacatcgatc cgcgcgggga
     2161 cacagcgagc acggcgacag cgttcgacat gcctttcgcg gccagcgtgg tcccgtcgat
     2221 cgggtccaca gccacgtcac atggggggcc ggtgccgtcg cccacgcgtt cgccgttgta
     2281 cagcatcggc gcgttgtcct tctcgccctc cccgatcacc acgatgccgt ccatccccac
     2341 cgtgccgatc agcgcccgca tggcgttgac agccaccccg tcggcgccgt ccttgtcgcc
     2401 ccggccgacc caacggcccg cagccatcgc tgcggcctcg gtgacccgga ccagctccat
     2461 ggcgaggttg cggtcaggcg cgttgctcgg cgtcttccag gtgtgatcca gcggcgcggt
     2521 cggcagggcg tcagacacgg aaggctctcc tcgtgatccg ggtgagccga tcgtaacgaa
     2581 gccggtcgcc gtacgcctgt ttgcctgacg gctcaaccac ttccggagga ctcggcaggg
     2641 tagatgctca cctcggtggc cttcaccgag gcccacacgt tccggccggg acgcaggtcg
     2701 agtgtcgcca ccgctgccgg tgtcacgtcg gcaagcagcg gcaacgccgc gtcgatctgt
     2761 atccggacgc tgtccccgtg cggggccaac gacgtgacgc ttccccgcca gacgttgcga
     2821 ggactgccgg cgggctcgtc gacgaagagg ctcaccgcgg ccggcgagaa cgccgcgaag
     2881 gctgcgcctc cgagcgcctc cccggcgacc actgctgtgg tctcgtcgag ccggatgacg
     2941 ccccggtcgg cctcgccgcg gaccaggttg agcccgacga gcgcggcaac gtggtgggtg
     3001 cgcggtcggg cggcgacctc ggacggcgta ccggactgca cgatccgtcc ctcgtcgagc
     3061 acgacgagac ggtccgccat gaccatcgcg tcgagcgccg tgtgcgtgac caccaggcag
     3121 acaccggcga actcgtgtag gtggtcgcgg agctgagtcc gcaacgtcat cgcaccggct
     3181 gcgtcgagtg acgcggtggg ctcgtcgagg agcagcatgc cgggattggc cgccaacgca
     3241 cgtgcgatcg cgacccgctg cgcctggccg ccagacagct gtcggggccg ccgatcggcc
     3301 aggtcggcga tcgagagatg ttccaaccac tcctgcgcgc gctgtcgtgc aggtctgcgg
     3361 cggatgcccc gcgaccgagg gccgaacgcg acgttgtcga ccgccgacag gtgggggaac
     3421 aacaggtggt cctggaagac atacccgacc gaccgctcct gcggcggaat gtgcacgtcc
     3481 gggctggaaa gggtgacgcc gtcgagctcg atcgcgccgt catcgacacg gatgaggccg
     3541 gccaacgctc gtagcgtcgt cgacttcccc gcaccgttcg ggccgatcaa cccgacgact
     3601 tcaccggcct gcgctaccag atcgatcgtg gtcgtgtggg caggacggcg tacgacgaaa
     3661 tcggcaacca gactcaccga gcagcgcccg agagccagcg ttcacgaagc agggccaaca
     3721 cagccaccga gactacgatc aggaccaggc tcagcgcgat cgcaccgtca ggactttcct
     3781 gcagcgccaa gtagatctgg acgggcatgg tccgggtcgt tcccgggaag ttgccggcga
     3841 acgtcacggt ggcgccgaac tcgcccagcg cacgcgccca gcagagcacc gcaccggcca
     3901 cgatccccgg catcgccagc ggcaacgtga cccgacgaaa cgtcgaccag cgcgaggctc
     3961 cgagggttgc ggcagcatcg tcgtaccgct cgtcggaggc gcggaaggcg ccttccacgc
     4021 tgatcacgag gaacggcagc gccacgaacg tctgtgccat gaccaccgcc gtggtcgtga
     4081 acggcaacgt gatgccgaac gcctcgtcga gccacctgcc gacgatgccg ttgcggccga
     4141 acgccatcag cagcgccaca ccgccgacca ctggcggtag caccaacggc agcgtgacga
     4201 gcgctcgcac gaagctacgg ccgcggaacg tcgtccgggc gagaacccac gcaagcggta
     4261 cgccgagcac gatgcaggcg aacgtcgccg tcaaggtggt ctgcaggctc aggcgcaggg
     4321 cgtcgcgagc ggcctgggtg ccgagctcgt cagcgagcgt cccccacggc gctcggacga
     4381 ggacggccac gaccgggacg acgaggaacg ccaatgccat caccgccgga acgatgagac
     4441 cgagcggtgg tcgcccgcgg atggcgatgc ccgaccggtt cacggtgcgg cgccgaaccc
     4501 gtcctgctgg agcacactct ggccagcctg cgaggtgacg aggtcgaccc actcctgggc
     4561 gaggtgggac tctccggagt ccttgatgac gccgatgaag tacgggttca cgacgttgac
     4621 atctggcgcg atgtccactg tcgagacctt gtcgccggcc gcctgtgcgt ccgtgacgta
     4681 gacgatgccg gcatccgcct cacccagggt gaccttggtc aagacggcct tgacgtcctc
     4741 ctccagcgac ttcggcttgg ccgtgatgcc ggcgttgtcg agaattgttg ctgctgccgc
     4801 tccacacggc accgacgggt cgcacacgac gtagtcggca tgctgcagat ccccgagcga
     4861 cctgacgttg ccggggttgt ccggcggggt cacgacgatg agcgtgttcg tcgcgaactg
     4921 ggttggctcc ccatcgagat cgccggcgtc ggtgacgatg cccatgctct tgtcgtcggc
     4981 ggtggcgatg acgtcggccg gcgaaccttc ggtcacctgg gtggcgaggt ccgtgctcga
     5041 cccgaacgac gtgaccacgt cgacgtcgga gtggtccttc tcgaagtcct tctccaaggt
     5101 cgtgaatgcc tcggtgagcg acgcagcagc gaacaccgtg agctgtgtcg tgtcgccacc
     5161 cccaccgtcg ccgtcactgc agctcaccag gcacgctgcc agcaggagag ccgctgaacc
     5221 ggccccgatg gttcggcgat ggatcggacg gatcaccttc atgtggggac ctccacgacg
     5281 acgttggttg ccttgatgga tgcgaccgcg aggacacccg gttcgagccg cagcgcgtcg
     5341 gcggcttcgc ggctcatcag cgacaccacc cggtacggac cgcaacagag ttcgacctgt
     5401 gccatcacac tgtcgcgctt gacctcggtg acgatcccgc ggagctggtt gcgggcgctg
     5461 acacggtgcg agcctcctgc cgtcccctcg atcggcaggt cggcaagctg gcgggcgagc
     5521 cgggccagtg caatgccctc gatcccggca tggccgtccg ccgtacgggc tctcgggaag
     5581 cgacccgcct ccgaccaccg ccgaaccgtg tcgtcactga cgccgagcag cgtggccgct
     5641 tcagcgaggc gatagacagt cacgccctgc accctatccg catctgcgtc cagcgtctcg
     5701 ctacgacctc accgcatctg cggactcgag actccgtgtc ggttgggtgt ttgcgatgtt
     5761 tacagcccag ccgggctgtc aacatcgcaa accctcgatg gagacagcgg gcttgcgatc
     5821 gatggttggg cccggtccgg acggggccac ctggacccgc tggcggatca ccggcgtatc
     5881 gcagtgacaa tggcggcgtg agccagactc gcagcgggta ccgcaccagc gtggctggcc
     5941 tggtcggcgc cctgctcgtg acccttccgt tgctggccgg ggtctacctc ttcacgctgc
     6001 tgggtcgcca cgaggcgccc gacccggtca cgcccgtgga ctacacggca gagctggcga
     6061 cagcgcgtgc ggaggcgccg ttcgccgtgc tcgcacccga gagcctgccg gcgggatggc
     6121 gggcgacatc ggtgaactgg gacggggccg gtcccgagaa cgcgtggcac ctgggcttcc
     6181 tcaccgacac cgatgagtac gtcggcctgg agcagggaaa cgccgtagcg caggagttcg
     6241 tggccgacaa gactccggcg gaccaacccg gagaacccgt ccgtatcgag gggacgatct
     6301 ggcagaccct caccgggaac ggtgacaccg cgctggtgca cgtcgaggac ggcgtgacga
     6361 cgatcgtcac ggggaccgct ccggagtcca cgctggtcgc cttcgcccga tccctccgcc
     6421 cctcctgacc tgttcgggcg tgggtttgcc tgggtagccg tgcaaacgca cggctagcca
     6481 ggcaaaccgg gggctcctca gtcttctgtg ggcggagacg gtcgtcatgc ctgggcgtgg
     6541 tcgcccggct tctgtcgtga tgagctcagg tcggtcggca atgagacgca ggaacgttca
     6601 cgtccgctga gccggtccgc cgacgttgcg tcccgtttgg ctggtgggtg actgcgggtt
     6661 tcggacatcc gttggtcgcc gagcggcggt ttgcatggct acccaggcga agccaccgct
     6721 cccgaggcgg atctgcctgg ctacccgacc atttggatgg ctgccgaggc aaaccaccac
     6781 cgacacgtgt ccgactagtg gtgaaggcgg gcttttcgga ggctgctcgg ccgcgcgacg
     6841 gaaggtcgcc tgaccagccc agcggcacgg acgcgtgatc tcttcgctgc cgaccaccgc
     6901 agctggggcc gacggagcgg cagccgatca cgcacaccat cgcgaccacc tccgcccacg
     6961 aacccgagga gtcgcaagcc cgccttcggg ctaactgcct tgctgaccac cgtcgggggc
     7021 gtcatgcgcc gccgacgtct cggccagtac ggcgtcgagc ctggcgcggg caccgtcgag
     7081 ccagcgctgg cagatcttgg ccagctgctc cccgcgctcc cacaatgcga tcgactcctc
     7141 cagcgtcgta ccgccggcct ccagctgttg cacgatgctg accagctcgt cgcgcgcggc
     7201 ttcgtagctc aactcgtccg aagactggtc ggatgcttgg tcgtcggtca cggatcgacc
     7261 ttagacgagt gccggacggt tcccacgcgt tcaagacgcg gcgcgggagt cggagcatga
     7321 tcgaggacat gcccacgtca ctcatcacgc aactgacacc tcggctggtc gtcgccgaag
     7381 cggcgaaagc catcgacttc tactgcaccg cgttgggcgc cgacgagcag gaacggttcg
     7441 aggtcgacgg cgcgatcgcc cacgctcgtg tcacgatcga cgggctcgat gtcgcgctca
     7501 aggatgccga cgagttcgac ctggctccgc cgtcgctcgg tgggacgccg gtcatcatct
     7561 cgctgaccgt gacggacgcc gatgcggtcg gccagcgcat ggtcgacgcg ggcgcaacgg
     7621 tcgtgttcga ggtcggtgac cgtgactacg ggttccgcga cggcaggctg cgcgacccgt
     7681 tcggccacct ctggatcgtg tcgcaaccac tgcgctgagg cggctcacga atcgtccggg
     7741 tagacctcgg cgaccgtccc gaggagccgc ccgtcagcca gccgggcgtc gatggtcgtc
     7801 cctggaccca cgtcggacac cgccctgacg acatgtccgt ccgttgtctg cacgacggcg
     7861 tacccgcgct gcaaggtggc cagtggtgac aacgcccgaa ccctggcgac ccgatgggcg
     7921 aggtcctcct cagcacgagc gagatggtga tcgaacgtac gccgtgagcg ggccagcagc
     7981 acgtcgacct gctcgctgaa ccggtcgagg acccgatgtg gctgcgccaa caccggccgc
     8041 gagcgcgtcg tttccaacgc atgcaactca cgcctcagca tgctgccgag cacgaaccgc
     8101 ccgcggtcgc gcaccgtgac cagcttggct gcctcttcgg cgacgtcggg cacgacggac
     8161 ttggccccgt cggtgggcgt ggacgcacgg aggtcggcca ccaggtcgag cagcggctgg
     8221 tcctcctcgt ggccgatggc gctcaccacc ggcgtcgtgc acgcggccac cgccctgatc
     8281 agaccctcgt cggagaacgg cagcaggtcc tcgagggctc cgccgccgcg cgagacgatg
     8341 atgacgtcca cctcctgctc cttgtcgagc acgcggagcg cctcgatgac ctggcgagca
     8401 gcgtcgtagc cctgcactgc ggcgtactcc gtgcggaacg aaacgccagg ccaacgtcgc
     8461 cgcgcgttgt cgacgatgtc gcgctcggct gccgaatcgc gaccgacgac caggccgatc
     8521 atccgtggga ggaagggcag tggacgcttg cgggccgtgt cgaacagtcc ctccgccgcc
     8581 agcaactgcc gacgttgttc gatgcgcgcg agcagctcgc cgatcccgac cggccggatc
     8641 tccctcgcca ccatcgacaa cacgccacgc gggacgtagt aggtgaactt ggcgtgcacg
     8701 acgacgcggg cgccttcggt caccggcgtc ggcatcgcat ccagcacggc gcggttgcat
     8761 gtcacctgga cggagacctc ggccagcggg tcgcgcagcg tcaggaagac cgtcgacgga
     8821 cggcgggtga gctgcgcgat ctgcccctcg acccagaccg ggcccagccg gttgatgtag
     8881 ccggtcatcg cgttcgcgat gacacgcacg ggcgcgggct tgtcctgggt tgtctcgagg
     8941 gccacccgac gaggttatcg accccgccgt ccgaacgtgg gacgcatcac acggcgcacg
     9001 ccgaggtggc gatcgcggtc cgacacgccg acgaaaagca acgaaacctg ccacctcggc
     9061 ggagttgggg gaacggtgag cgcgcaccta ccatggcagg catgtccagt gtggcctccg
     9121 gttcttcgag tacgccgtcg agggcgtcct cgacgtggtc gaccccgcag ccggacggtc
     9181 gccaggtcct gctggctgca ccgcgtgggt actgcgcagg agtcgaccgg gccgtgatca
     9241 ccgtggagaa ggcgctcgac gtctacggac caccgatcta cgtgcgcaag cagatcgtgc
     9301 acaacaagca cgtcgtgcgg acgctcgaga accgtggcgc gatcttcgtc gaggagctcg
     9361 acgaggtccc cgaaggatcg accgtcgtct tctcggcaca cggggtctct cctgccgttc
     9421 acgagcaggc gtccgaacgc cggctcaaga ccatcgacgc gacctgcccg ctcgtcacca
     9481 aggtccacaa cgaggcgcgt cggttcgcca acgacggcta cgacatcctc ctcatcggtc
     9541 acgaaggcca cgaggaggtc gaagggacct cgggcgaggc tcctgacaac atcaccctgg
     9601 tccaggggcc aggcgacgtc gccgacctgg acctccgcga tccgcagcgg gtcgcgtggc
     9661 tgtcgcagac cacgctctcc gtcgacgaga ccatgcagac cgtggaccgg atccggcaac
     9721 gcttcccgaa cctgcttgac ccgccttcgg acgacatctg ttacgccacc cagaaccggc
     9781 agcaggcggt gaaggagatc gccaatggcg cggacctggt catcgtcgtc ggttcgggaa
     9841 actcgtcgaa ctccgtccga ctggtcgagg tggcgttgga ggcgggagcc caggcggcgt
     9901 accgcgtgga cgacgcctcg gagatcgacg aggaatggtt ggagggggcg gactcggtcg
     9961 gtgtgacatc cggagcctcg gtacccgagg acctggtaga gggtgtgctg gacttcctcg
    10021 gcgaacgcgg ctacccgatg gcctcgtcgg tccacaccgc ggaggagtcg ctcatcttcg
    10081 cgctgccgcc ggagctgcgt cgcgatctgc gggaagcgca accggccaac gactgagcga
    10141 ggccgcgcga tgacggtgcc tcagcgccga cggtgctcga gcgtgcgtcc cgccaccgtg
    10201 acgagtgccg ttccgtagcc gaaggccagc gcgccggcgt gcgaggtcag cccggtgagg
    10261 aacgtcttgc tcaggttccc ggcggcgatg atcgtctccg gcgccgcgat cgtcacgacc
    10321 gccaccgtcg ctccgaacag caacggcggc aacacccccg tcgtgaagag gtcatggccg
    10381 cgcaccgcca tcgcggtgac cagacagatg accacgaagg tcaggtcgaa gaagaacgac
    10441 agccgacccg tcagcgcgaa gtcgagcacg gccgtccccg ccgtcgccag gcctgccatg
    10501 aggacgacac cccggcctgg catgcccggg agcgactcgt acaccgtggc ccgacggccg
    10561 gactcgaccg gctccggttc gtaggagtcg tcaacgtagg cggacgcgga agaaggggag
    10621 tacggcgcct cgccgtagcc ctgatcgggg aggtcgacgt cctccgcctc gggctcgagc
    10681 gtgccgagta gggcgacgtc ggcctcggac gcgagaacgt ccggagccgg gtgcggcgag
    10741 ctggccatcg tcgcctccct gagcctggtg gtgaccccac ggtacggccc agggccaccc
    10801 cggtccgcag cgccacgccg accacaccag cctcaccagt cacagacttt ggctggcggg
    10861 agctcctact ccccgaccgc gtgccgactg aactgttccc gacccgggtc gccttcgatg
    10921 gccgggctcg ccggctcagg tcggtcgagc agctcagcgg cggtgagcgg tcggggcagg
    10981 tcggtgacgg ccggcgtctc cggcagggac ttctcgatgc cttgcaggtc tcgcatccgc
    11041 cgcgcgctga cgaggaccct cgtctccatg gaggcgacgg cctggttgta gctcccgaca
    11101 acggccgaga tcgaccggcc gagcttgtcg aggtggctgc ccaccgtgcc gagccggtcg
    11161 tagagctcac gtccgagcgc gtgaacctcg cgagcgttgt ccgccaacgc ttcctgcgtc
    11221 caggcgtacg acaccgtctt gagcatcgcg atgagcgtcg tcggggtggc gaggacgacc
    11281 cccttgcctg cggcgtactc gatcagcgtc ggatcggtat caagcgcctg cgcgaagatc
    11341 gcctcggccg gcaggaacat cacgacgaac tccggtgccg gcgagaactg cagccagtac
    11401 gccttgccgg ccaaggcgtc gatgtgcgca cgcacctggc gggcgtgccg ctgcagctcg
    11461 gcatcgcgcc tggctccctg cacctcggtc gccgagagga aggcgtcgag cggaaccttc
    11521 gcatcgacga cgatctgttt gccaccggtc agctgcacca cgacatccgg tcgcagccgg
    11581 ccgtcgtcgg tctgtcgggt gacctgctcg tcgaacgcgc agtgcgccga cagcccggcc
    11641 agctccaggc tgcgtcggag ctggagctct ccccacgtgc cgcgcacatg tggccggcgc
    11701 agcgcctcgg ccagcgccgc ggtctgctgc cgcagctcga tgcccgacag gtgcacggac
    11761 tcgacctgct gcttgagctg cgactgccac gacgcacctt gctgttcgag cgtgccgatc
    11821 cggtcgttga gccggtcgag gctctcgcgg accggctgca cgacctgctc ggtggcgcgc
    11881 acccgcgagt cggccagtgc cagcacctgg ttcgtcgact cgctcaccgc ctgtgcggac
    11941 agcaggcgca gctgctcgtg caggtcagac ctggatcgag cggccagcca caggccgacg
    12001 accacgccga tggcgaggcc ggccgcgccg gtcagaccgg cgatgacgaa ggtggtcgcg
    12061 ttcatggcgg agaccatgac agacgggtcc gacaaaacgt gggaactccc cagcggacga
    12121 cctcccgcgg cggcaaatca cgcgtcgctg gcaggcttgg acctcccatg cctggcgccg
    12181 cgtcggcacg atggcgagat gaccgactac cgagccgtcg tcgacgccga gatctcgttc
    12241 gccaacggtg gcggcatcag cgtcagcgga ttccgcctgg acatccccgg ccaggacacc
    12301 gggcgtgacg agatcggcca gctcctggtg cgctcgctcg ggttgttgat ggccgacgac
    12361 gtccgcatgg gctcgatcga gatcatcgcc gagcagcaca agggcacgcg cggcggaccg
    12421 gctgacgtga gcgccggtcg caccggccgg ggacgcggcc gtctggtcga cctgagccac
    12481 ccgatccgcg cgggcatggt gacgctgccc gggctccccg gccccgagat caccaggcat
    12541 ctcacccgtg aggcgtccaa ggccatctac gcgcccggta cgacgttcga gatcggccgc
    12601 atctcgatgg tcgccaacac ggggacgtac gtcgacgcgc cgcaccacag gttcgccggc
    12661 ggtgtggatc tgtccggact cgagctctcg tcactcgctg acctgccgac gatcgtggtg
    12721 cggctccggg acaccccacc cggcgggatc ggcgtcgagg ccctcgcggc atacgacgtc
    12781 aagggtcacg ccgtgctgct cgagactgga gacagcgacc gcttcgggga cccggagtac
    12841 gtggagaacc cgtcgtttct gacccgggac ggcgcgtcct ggctggtggc gcacggtgtg
    12901 caactcgtcg gtatcgacgc cgcgaacatc gacgacatgg gcgacggcac ccggcccgcg
    12961 cacaccctgc tgctccaggc cgggatcccc gtcgtcgaac acgtgaccgg cctttcggag
    13021 gtgcccgcga cgggtgcgcg gttctttgcc gtgccaccca aagtggccga tttcggaacg
    13081 tttccggtgc gtgcgttcgc gttgatccct gcctgagtcg cggcgtgggt cgaaagcgac
    13141 ccggaaggct tgcgccaacg gcggaactgc cccggaactg tcggtggccg tcgctagtgt
    13201 cgacgacatg actggtgatg agcgtgcggg tggcatcgtc gacgtgggac gtgtcaccga
    13261 acggggcgac cgcgccgtcg agggggcgtc cggaggtggc gaccgcaggg ccggcacgga
    13321 ccgtacgccg gcggacgagc cgaccaggag gttgacgcgg acgacccagt gatggagacc
    13381 gagcgcaccc ggcttcgcgg ttggcgtgcg gacgatgtcg accgagtcct cgacatctat
    13441 tcccggtggg aggtctcgaa gtggctcggt gccgaaccca aggtgatgca gacgcgcgag
    13501 caggccgagc agttcgtcga gcgcgtgatc gcgcgggagc tgcgtcgcga cgcgaccggc
    13561 ttctgggcgg tcgaacgcaa ggcggaccag gtcgtcgccg ggacgttgct gctcatgccg
    13621 ctgcaaggtg gtgagcgtga cgaggtcgag atcggctggc acttccaccc ggattcgtgg
    13681 ggacaagggc ttgcctcgga gtcggcgcgg gcgttgctga cctggggctt cgagcacggg
    13741 ttggacgaga ttctcgcggt cgtgcgcccc ggcaacgccg cctcgatggc ggtctgccgc
    13801 cgcatcggca tgacccacct cggccgcacg tcgacgtact acgccaccga gctcgagctc
    13861 ttccgcatca accgtccctc caccgccgag gtggcaactt tcgttcatat ttgacggcgt
    13921 gtcggttctc ggttgccacc tcggtcgcgg aggttctgca ccgcccaagc cggatttcga
    13981 gtcgagccgc cgtacgccgt acccttgtcc gccgtggcac tcaccatcgg gatcgtcgga
    14041 cttcccaacg ccggcaagtc gacactgttc aacgcgctca ccaagaacga cgtgctggcc
    14101 gccaactatc cgttcgccac gatcgagccg aacgtcggcg tggtcggcgt acccgacgaa
    14161 cgtctcggca tcctcgcgaa gctcttcgag tcggcgcggc aggtcccggc aaccgtgcag
    14221 ttcgtcgaca tcgcgggcat cgtgcggggc gcgtccgaag gggaggggct cggcaacaag
    14281 ttcctctcgc acatccgaga gtccgacgcg atctgtcagg tgacccgcgt cttccgcgac
    14341 gacgatgtca cgcacgtcga ggggaaggtg tcgcccgccg acgacatcga gacgatcaag
    14401 accgagctga tcctggccga cctgcagacg gtcgagaagg cggtcccgag actggagaag
    14461 gaggcccggc tgaggaagga gatgcagcct cagctcgacg cggtccgcga ggcgcaggcg
    14521 gcgttgtcgg cgggtacgcc gatcatcgcc acctcgatcg acaagtcgca ccttcgcgag
    14581 ctcatgctgt tgaccgccaa gccgtacctc tacgtcttca actgcgacgc cgacgagctc
    14641 gccgacgagg agctcaaggc cgaaatgcgt gctgtcgtcg cgccgtcgga agccattttc
    14701 ctcgacgcca agttcgaggc cgagctggtc gagctcggcg acgacgacga agcccgggag
    14761 atgctggcgg agatggggat cgacgagccc ggcctcgaca cgttggcgcg ggtcggcttc
    14821 gacacgctcg gcctccagac gtacctcacc gcaggcccga aggagtcccg cgcctggacg
    14881 atcaagaagg gcgcgaccgc tccggaggcc gccggcgtca tccacaccga cttccaacgc
    14941 ggcttcatca aggccgagat cgtgtcgtac gacgacctgg tggctgccgg ctcgatgaac
    15001 gcggcccggt cagccggcaa ggttcggatg gagggcaagg actatgtcat gcaggacggc
    15061 gacgtcgtcg agttccgctt caacgtgtga attgctgcgt ttccgcatca gacagtgtcc
    15121 tccggcttca atcggcgacg accaacaacc accgaaaccc cgcgtggtct tccctttcaa
    15181 cgtctgacca ctccgcccac gttgatgacg ccgtgaggtc ggagatgagg gcctgtgctg
    15241 tcatggacgt atgtcgcctt gggagccggt gcccagcccc gcgccaggtg acgaggaacg
    15301 agacgtcgtc cggatggacg tcgacggcga agagttcgac gtgagcgagc gaccggaccg
    15361 caccggggag tatcacttcg actgggtctc cggcccgaac gcggggtatg gcttcaccac
    15421 ggcgacctcg gaccacagcg agctggacca ggccgaactc gaagaggcga tccgtgagtt
    15481 cctcgccatg gtcgatcccg agaccggata catcgagtag cgcgcccgac accgatcgca
    15541 cctcggtggt ggcgttccag tgcgggccgc agaacgacgc agtcgttcgt ctagcctggc
    15601 cgacatgacg acgcccatcg agccccagac catcgaacga gccgagcctc ccttcgccgc
    15661 ggatgaacgc acgatgctcg acgcgtggct ggacttccac cgtgcgacgc tgctgacgaa
    15721 gtgcgagggc ctcaccgagg accagctccg cacccgcgca gtgccgccgt cctcgttgag
    15781 tctgctcggc ctggtccggc acatggctga cgtcgagcgg agctggttcc gtcgtgccct
    15841 cgccggagaa gcccgcgacg atgcgccacc gcagtactac tcggacgaca accccgacgg
    15901 agagttcgac gacgtggaca cggccgacgc gctggccgac ctcgctcgct accgggacga
    15961 ggtcgcgcgc tgtcgtcagg tcgctgccgt ccacaccgac ctcgaagcgc tcggcaacgg
    16021 cgtgcgggac ggccggcggg caccgatgtc gctgcgctgg atctatctgc acatgatcga
    16081 ggagtacgcc cgacacaacg gtcacgccga cttcctccgt gaacgcatcg acggggtcac
    16141 cggcgtctga gcccgaggcg accacgtctc gaagtcgccg tcgaaagtga catcgaaggg
    16201 cgatgtcgaa aacgcggcga cgtgtccgac gtagaggtga caagcaggct ccgcacagat
    16261 gggatgagac agatgcagta catgtcattc gtgaagatga ccggcgacgg cggtgagccg
    16321 ccgaaggaac tccaggacgc gatgggcgag gagatcgaac gcgggttcgc cgacgggacg
    16381 ttggtccaca tcggtgggtt gtacgcgacc tcgtaccgca ccgagttccg cgtccgggcc
    16441 ggtcaggtga cctccaccga cggcccgttc agcgaagcca aggaggaggt cggcggattc
    16501 gccatcatcg acgtgcgtga ccacgccgaa gcggtggaga acgcccggcg gctggcagag
    16561 ctgcaccaca agctgtggcc gggctgggag gggtcggtcg aggtacgccg gatcgcgggt
    16621 gctgacgaca tcgcaagctc gggggatgac ccggcggcat cggtctgacc gatcgacgcg
    16681 ggtctggtga tggtcggctc gccctacgct gcatctgtgg gcgagacgac cgaccctgag
    16741 accagcgacg gaccagacat gggcagcgcc gcggtcaccg ctgtctggcg ggtcgagtcg
    16801 acacgactcg tcgcggcgct cacccggatc accggtgacg tcggtctcgc cgaggacgtc
    16861 gtccaggacg cgttggtggc ggccctcgac cagtggccgc gcgacggcgt acccgacaat
    16921 cccgccgcct ggctgatgac gaccgcgaag cggcgggcga tcgaccactt ccggcgttcg
    16981 gagtcgctgc gccgtcgtac cgccgagctc ggccatgggc tggaggaggt cgaggtgccc
    17041 gacctggacg ccgccgtcga cttcatcgag gacgacgtga tccggctgat cttcctgtgc
    17101 tgccacccct cactcacggc ggactcgcgg gcagccctga cgcttcggct ggtggtcgga
    17161 ctgagcacag ccgagattgc tcgtgggttc cttgtcgcgg aagcgaccat gggtcagcgg
    17221 atcacccggg ccaagcgcac gctggcgtct tcccgtgccc gcctcgagct gccgacgggc
    17281 gccgaccggg atcgacgact cgatgacgtg atggcggtca tctacctgat cttcaacgag
    17341 gggtacgccg cgacggcagg cgcggagtgg acgcggccgg acctgtgcca cgaggcgatc
    17401 cgactggccc gcgtcctggc cggactcgtg ccggacgagc cggaggtcca cagcctgcag
    17461 gcgctgctcg agctgcaggg ctcccggctg cacgcacgtg tcgacggcga aggccagccg
    17521 gtgctgttgg cggatcaaga ccggcgacgc tgggatctgc tgctcgtccg tcgcggtcag
    17581 gcagcgttgg cgcgggccga gcgactcgcc gagaagggcc gacccgtggg ccggtactac
    17641 ctccaagcgg cgatcgcggc cgagcacgcc cgagcccttc gcggcgacga caccgactgg
    17701 gcgacgatcg cccagctcta cgacgtcctg gccgaggcag cacctggccc ggtcgtcgag
    17761 gtcaaccgtg ccgttgcgca cggccgcgcc ttcggcccgg aggccggtct ggccgtgctc
    17821 gacgcgatcc ctgctgacga actcgagggg tcgcacctcc cggcggccgt acgcggcgac
    17881 ttgctggtcc ggctgggccg tcgcagcgag gctgcagcgg ccttccgtac ggccgctgcc
    17941 ctcacccgca accagggtga gcgcatcctg ctctcgcgac gcgccaccga cgcggacgac
    18001 atgacgacgc cgaggtggca ggtttcgttg gattctgccg gtgtgtcgga ccgcgattga
    18061 cacctcggta cccgccgatc gacccgcgtt gagatcaaga acccgcaggt ggcgtgttcg
    18121 gccggactcg gcgacccggc cgccggactt cgtcggtcga cgggctcgac cgctgaatgg
    18181 ccgataccgt cggaacgtga gagcgatcga gtacgccgtc accggtggcc ccgaagtctt
    18241 gatgctcgtc gaccgagaga tcccggaacc cggcccgggc gaggtacgtg tgcagatcca
    18301 tcgctccggg gtcaacccga ccgactggaa gtcccgccga ggcgaacgcg ccggcagcaa
    18361 ggtcgatcct ccacaggtac cgaaccagga cggctccggc gtcgtcgacg cggtcggcca
    18421 aggcgtcgag gcggcgctgc gcggactacg ggtgtggatc tgggaagccg cgtaccaacg
    18481 tccttccggg ggcacggcgc aggagtacgc cgtcgtaccg gctcgacacg tggtgctgct
    18541 gccggacacg gcgtccttcg acgtcggcgc cagcctcggc gtgccgttcc tgaccgcgca
    18601 ccggtgcctg acggtcaccg aagacgggcc gacacgcctc gggcccggaa cgatgcaggg
    18661 ccgcacggtg ctggtggcgg ggggagccgg cgccgtcggc aacgccgcga tccagctcgc
    18721 ccgatggtcc gacgccacgg tgatcacgac cgtcagcagt cccgagaagg cgcacctcgc
    18781 cgcacgggcc ggagccgacc acgtcatcga ctacacgcgg caggacgtcg ccaccgaggt
    18841 gcgggcgatc gcaccggacg gtgtgcacac catcgtcgag gtctccccag cggtgaacgc
    18901 ctcgctcgac gcgcagatcc tggcccggca cggctcggtc gcggtctacg ccaacaacgg
    18961 gggcaacgag gtgcagctgc cggttcggac gtcgatggtg cccaatgcgc gatggcagtt
    19021 cgtgctgctc tacaccgcgc cggacagctg gcgcgagcga ggcctggagg acctggctgc
    19081 ggcagtgctg gacggcgccg cccgtgcggg tgagcaggca ggcctgccgc tgcaccattt
    19141 cccgctcgag gaagcagccg ccgcacaccg tgccgtcgag gacggcgcag tcggcaaggt
    19201 gctcatcgac gtacggccct agcgcgcgac tcgcgcgatc gggcccgcca gagacgttgc
    19261 gcgcggtgtc ggacgggtcg tggcggtggt gacgatcgcc gcgagctcgt cccgggtcgg
    19321 accctccggt ggcgtcgggc gccatccgcc cgcgtagagc tcccgcgccg tcggctcgat
    19381 ggtgtcgagt gccgacggcg gcaccagcat cgcgagatac ggcccgacga cacgcatgag
    19441 tgacggatcc gcctctgccg cctggatgat caggtccgac ggcagcggtc gtgatacgtc
    19501 gacgtcaccg ccggaccagc ggcggatctg gtcggcgtcc caccgcgcat ggtcgtcgaa
    19561 ccacggcttg atgtggtccg cacaccagcg atcgaactcc ctcgagacgg cgcgcaggtc
    19621 gccgtcaccc gtgtcgagaa gccgcagcag ctcctggacc tgcagcatcg acagcgccac
    19681 cccgcggccg agagccgggt tggtcgtgga gacggcgtcg cccacgaaga gcaggcccga
    19741 cagggctgtc tcaccgtgct cgtcgagctg gccgcggtag gtgttgaaca gccggccacc
    19801 tggcagtacc ggcgtgatcg gtcgggcccg gtccgggtcg gtccaagcgg cgaggatcgg
    19861 aatcgcccgc gcagcggcat cgaacgcggc agtgtcccga agcagcgcga ggtcacggtc
    19921 ggtgctggct cgcacgatca gggtggagaa cgtccggttg tcctgggtga acaccatcgc
    19981 ctggtagccg gcgtacatcg cgatggcgcc gatgggtgcg ttcatcgggc cgggtcgggc
    20041 tcctggcagc agctcgtact ggcgtgagac gtaggaaatt cccgagtctc cgccctcccc
    20101 cttcgcccgg aactcgtcgc cgagccggcc actgcgtcct gatgcgtcga tgaccaggtc
    20161 ggcgccgagc aggtctccgt ccacgcgtac gccggtcacc cgtccgcggc gaccgtagat
    20221 cctctcgacg tttccgacct ggaggtgcac gcctggttcg ttctcggccg ccgcccggag
    20281 ggcccgctcg tagacgagtc gtcggacgcg gacgcccacg aacatgtcgg gttgctccgg
    20341 ccgggtcgcc gggacggcac ccgcctcgag gagccgcacc cagacgtccg gcatctctgc
    20401 gcgcagtgcg tcgaccacct gctgccggaa gccgtgcgga tggtggaact gcatgacgcc
    20461 cttgcggtcc cagctgccgt cagcggctgg cccgctgtcg cggtcgacga cggtgacgtc
    20521 gtacccgcgc cgcgccaggg cgatggcggt gaacaagccg gtagggccgg ctccgatgac
    20581 gatggttcgc acagcgaggc tccgatcagt cgttcaggtg gtgagtgaag taatcgttac
    20641 actaaaagtg tagtgataac actcatgata gtctcacccc atgagcgagc ccgtcaagcg
    20701 gaattactcg tccaccctcc gcgagaccca agcgagtgag acccggcggg tgatcgtgtc
    20761 ggcggcgacg agactgttca tcgagcaggg attcgggcgt acgacgatcg acgccatcgc
    20821 agcggcggcc ggtgtgagcc ggaagaccgt gttcacgtcg gccggcggga aggccgacat
    20881 cctcaagctc gcgctcgact gggcagtggt gggtgacgac cgcccggttc cgctgtcgcg
    20941 tcgatcagcg gtggaacggc tgaagaagga gaccgacccg gcgaccctgg tcgaggggtg
    21001 gatcgagatg ggcgtcggga tctcggcacg ggtggccgga atctacggtg cgctggtcgt
    21061 cgccgccggc atcgacgacg atgcgcgcga tctgctggag actgcccggg ctcaacgctt
    21121 ggcaggggca cgcgcgatcg tgcgcgccct ggacgccctc ggcgccttgc gtccggacct
    21181 gagcgtcgac gaggcggccg acatcgcgtg gacgctcggc gacccgacac tgtacgagga
    21241 cctggtcgtc caccgcaggt ggtcacagcg tcggttcaag acgtggatga ccgacaccgc
    21301 ggcccgccag ctgctcgccg ctgcaccgag ctgtcagaag taggaggcgc tgaagcctcg
    21361 tgtgcttctg acaactcgga cagcggtcgg gggtcaggac accgagagcg agagcgtgcc
    21421 cagcaggccg tgtgactcgt cgtcggggcc ggccgtgaag aggacctcgt cggtcccgcc
    21481 ttccgtgccg ttgcccggcg tgagcgccca cagaccgtcg atgcggatct gtcggtggtg
    21541 ggtgtcgagg agtgccccgc gtggtgccag cgactgcgcg tcgaaggcgt gaatacgccc
    21601 gtcgccgaag ttgccgacga gcaggtctcc actgagcttg ccgaacccgg ccggcgcgat
    21661 cgtcatgccc cacggtgcat tcaggtcgcc gtggtgcgag agccgcttca gcagtgtgcc
    21721 atccgtcgag aacacgtcga cccggccgcg tccgtgcccg gcgacctcgt cctcgcggtc
    21781 cgcgtcctgc ttggcgtagg cgacatagac cttgccgtcc aggacggcga cgttgaacgg
    21841 gccgtagccg gccggcagtc ggcggtcctc gaagttgcca gccgtgatga cgcgctggaa
    21901 cataccgttg tacacgtcga cggttccggc cgagaagtcg gcggcgtaca acatcggtcc
    21961 gtcagctgtc tgcgccagcg ccaggccctt gtagaccgcg ccgggtgtga cgacggcgtc
    22021 ctgggcctgc gtggacatcg gcggcggaac gttcggcgcc cagcccgaca ggtggcccga
    22081 ctcaccgacg aacaggaacc tggcggtgcc gctgttcccg ctgtcgtcgc tcacgacgaa
    22141 gtcgctggtg gggttgacga cctggccggt gggcgcaccg ccgggaatgc tcaccaccag
    22201 ggacaggatg ttctgttggc caccgtggac acctccggtg tacagcgtcg agacgtcggt
    22261 gccgttgtcg gagacccaca gcgggctcgt ggatgagaag gagacccccc agggattgac
    22321 gaggttcggg tcagtgacct gggcc
//
LOCUS       DATBFZ010000314         6891 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_2184419, whole genome shotgun sequence.
ACCESSION   DATBFZ010000314 DATBFZ010000000
VERSION     DATBFZ010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 6891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6891
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2184419"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            164..874
                     /locus_tag="VH419_09675"
     CDS             164..874
                     /locus_tag="VH419_09675"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017933184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY7043922.1"
                     /translation="MEQIRRGLAAGAIVSPNIWNDPATYEVENRAVDPDGVIESTMQS
                     LRSYDGSVLLDVGCGTGFHLPRFASRAAHVIGVEPHPALARHARTRTADLANVEVRSG
                     AAQHLPVPTASVDVVHARWAYFFGPGCEPGLREVDRVLRRGGAAFVIDNDGSQSTFGR
                     WFRWEHPAYDTEAIDGFFARYGWSSHRRMIRWSFDTRADLEAVVRIEFSRQVAKRILC
                     EHSGTDVDYAIVVRHRGR"
     gene            928..2358
                     /gene="radA"
                     /locus_tag="VH419_09680"
     CDS             928..2358
                     /gene="radA"
                     /locus_tag="VH419_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004810510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEY7043923.1"
                     /translation="MPKTASAPRRPATTFRCGACGWTSVKWVGRCGECQAWGTVEEAG
                     APRIAPVAAGPVAAAAVPISQIAAQPAAARPSGVAELDRVLGGGIVPGAVLLLAGEPG
                     VGKSTLLLEVAACAARRGQRALYVSGEESASQVRLRAERTGALEDSLFLAAEVDLAAV
                     LGHVEAVGPDLLVIDSIQTVASGEIDGVSGGVTQVRAVTAALVRVAKERHLPILLVGH
                     VTKDGSIAGPRALEHVVDVVLQFEGDRNSRLRLLRAVKNRFGPVDEMGCFDLSDGGIV
                     EVADPTGLFLSRHPDPMSGTCVTVTLEGRRPLLAEVQALVTPTTVPSPRRAVSGIESA
                     RLAMLLAVLQRRVGLGVQSADVYASTVGGAALRDPATDLAVALAVASATVNVAVDEGT
                     VAIGEVGLAGELRPVPNIGQRLTEAARLGFTYAVVPSHHSSPSARLPTIDAMTLIECP
                     DLHRALTALGIAHQPPRPGQADLQIV"
     gene            2446..3282
                     /locus_tag="VH419_09685"
     CDS             2446..3282
                     /locus_tag="VH419_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY7043924.1"
                     /translation="MTVPTRDLLDGTRLPAIGFGTYPMKGAEAVTAIGNAIDVGYRLL
                     DTAVNYRNEADVGEAIRRSGVPREELFVTSKLPGRHHAYDDAIASTRGSLQRLGLDYL
                     DLHLIHWPNPSVGKYHEAWRALVQLQKDGLVRSIGVSNFNRDHLERIIDDTGVTPTVN
                     QIELHPYFPQETMRRVNADHGIQTEAWSPLGKRSAPFTEPVVAGLAEKYGVTPGQVIL
                     RWHVQLGSIPIPKSASPERQRTNIDVFGFELEAGEVDAITGLGRRNGRLFGGDPARHE
                     EM"
     gene            3512..4597
                     /gene="disA"
                     /locus_tag="VH419_09690"
     CDS             3512..4597
                     /gene="disA"
                     /locus_tag="VH419_09690"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020579476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning diadenylate cyclase DisA"
                     /protein_id="HEY7043925.1"
                     /translation="MATGDRHSPEARLRATLAAVAPGTELREGLERVLRARTGALVVL
                     GNDRTVESLCTGGFALEIDFTATALRELAKMDGAIVCDREATRIIRAGVHLMPDAVIQ
                     TQETGTRHRTADRVARQTGFPVISVSQSMDLIALYVGDTRYVLEDTGSILSKANQALA
                     TLERYKLRLDEVSGTLSALEIEDLVTVRDVASVAQRLEMVLRIAKEIEDYVLELGTDG
                     RLLALQLDELVAGVDHERELVIRDYLPTGRRARTSDSVLTDVDAVTATELLDIATVAR
                     ALGLGGAESLDSAVSPRGFRLLAKVPRLPGAVVERLVDHFGTLQKLLAASIDDLQAVD
                     GVGDSRARSVREGLSRLAESSILERYV"
     gene            complement(4717..5091)
                     /locus_tag="VH419_09695"
     CDS             complement(4717..5091)
                     /locus_tag="VH419_09695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043926.1"
                     /translation="MAIDVADSAPGQANTATILFTSTRTPACTLAITPDTMTVRITSG
                     PDVVWSSDDCPDSLPVKQVVARADPPTAYEFRWNGRRSAENCQPVDALPQPGGYWLEA
                     ALIGGEPHKAYFDITDDKPAKR"
     gene            5090..5269
                     /locus_tag="VH419_09700"
     CDS             5090..5269
                     /locus_tag="VH419_09700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043927.1"
                     /translation="MTTPAGLQSPVGSASVVFGCDFGAFVPLSVAVDDVVDDVVDDMV
                     DDVLDDDTLPGCAPG"
     gene            5300..5503
                     /locus_tag="VH419_09705"
     CDS             5300..5503
                     /locus_tag="VH419_09705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043928.1"
                     /translation="MVETLLVGVEAGEEAGVDDVGEDDVLDAGGAEAFGGVSFALPEP
                     LTSHHNDPARTATTTRSTSRRVQ"
     gene            5642..6526
                     /locus_tag="VH419_09710"
     CDS             5642..6526
                     /locus_tag="VH419_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020579473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEY7043929.1"
                     /translation="MPGRDIPLVHKRILAWYDDHRRALPWRDPSAEPWAVMVSEVMLQ
                     QTQVARVLPVYDAWMRRWPRPSALAAEPAGEAVRMWGRLGYPRRALRLHAAAVAVDAE
                     YDGIVPRTYDGLRALPGVGDYTANAVLAFAYRQRAVVLDTNVRRVHGRVFGGVPCPPR
                     AVAAAERRTAAAVLPDEAETAARWSVAVMELGALVCTARSPMCAVCPVADACSWRAAG
                     YPGQAGRVRRGQSYAGTDRQCRGRILGVLRDSDDPVSQESVAAVWDDADQRSRALASL
                     IADGLAVERPDGLLSLPS"
     gene            complement(6583..>6891)
                     /locus_tag="VH419_09715"
     CDS             complement(6583..>6891)
                     /locus_tag="VH419_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008354870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-hexose 4-ketoreductase"
                     /protein_id="HEY7043930.1"
                     /translation="EIRMKDRDMALELTQAAKELLARRGFDPVLGARPLRRTVQRDIE
                     DVLAEKMLYGEVGPGQIVLVDVEAGTDPGGEQQFSFTGTPKSELPDAPPIEEALPPSP
                     "
BASE COUNT          991 a         2366 c         2434 g         1100 t
ORIGIN      
        1 gtctcttcct ggctcgacac accgtacgaa caccggatcg gtgacttcga cgtggagtgg
       61 gtggcaccga acggcacccc ggggatcatc gccgccacct tcagcactcg aaacggcctc
      121 gtccgcatct gacgcagggc cggccgaggc aaccgctccc gccatggaac agatccgccg
      181 tggtctcgcg gccggtgcaa tcgtcagccc gaacatctgg aacgacccag ccacctacga
      241 ggtggagaat cgtgccgtcg acccggacgg cgtcatcgag tcgaccatgc agtcactccg
      301 gtcgtacgac ggctcggtcc ttctcgacgt cgggtgcggg acgggcttcc acctgccccg
      361 cttcgcgtct cgcgccgcgc acgtgatcgg cgtagaacca catccggcgc tggcgcggca
      421 cgcgcgaaca cgcactgcgg acctggccaa tgtcgaggtc cgctcgggtg cagcgcagca
      481 cctgcccgtg ccgacggcga gtgtcgacgt cgtgcacgcc cgatgggcct acttcttcgg
      541 tcccggctgc gagcctggac tccgtgaggt cgaccgcgtg cttcggcgag ggggtgccgc
      601 attcgtgatc gacaacgacg ggtcgcagag cacgttcgga cgctggttcc gctgggagca
      661 tccggcgtac gacaccgagg ccatcgacgg gttcttcgcc cggtacggct ggtcgagtca
      721 tcgtcggatg atccggtggt cgttcgacac acgagcggac ctcgaggctg tggtgcggat
      781 cgagttctcg cgtcaggtgg ccaagcgcat cctgtgcgag cactcgggca ccgacgtgga
      841 ctacgccatc gtcgttcggc atcgcggccg ttgacggaca ggcgtgtcgg tcgggcgttg
      901 tcggcaccgg cgcctagcgt cgtcgccatg cccaagaccg catcagcccc ccgcaggccg
      961 gccacgacgt ttcgctgcgg agcgtgcggt tggacgtcgg tgaagtgggt gggccgctgc
     1021 ggcgaatgcc aggcgtgggg caccgtcgaa gaggccggcg caccgcgcat cgccccggtg
     1081 gccgccggtc cggtggcggc tgcggccgtg ccgatctcgc agatagcggc acagccggcc
     1141 gccgcccgac ccagcggcgt cgccgagctg gaccgcgtgc tcgggggtgg catcgtgccc
     1201 ggggccgtgc tgctgctggc cggcgagcct ggcgtcggca agtcgaccct gctgctcgag
     1261 gtggccgcgt gcgcggcccg gcgcggacag cgggccctgt acgtctcggg cgaggagtcg
     1321 gcctcccagg tgcggctgcg cgccgaacgc accggagcac tggaggactc cctgttcctg
     1381 gcagccgagg tggacctggc ggccgtcctc ggtcatgtcg aggccgtcgg tccggacctg
     1441 ctcgtcatcg actcgatcca gacggtcgcg agcggcgaga tcgacggtgt ttcgggcgga
     1501 gtgacccagg tccgtgcggt cactgcggct ctggtgagag tggcgaagga acgccacctg
     1561 ccgatcctcc tggtcgggca cgtgacgaag gacggctcga tcgccgggcc acgcgcgctg
     1621 gagcacgtcg tcgatgtcgt gctccagttc gagggcgacc gtaactcacg gttgcggctg
     1681 ctacgcgcgg tgaagaacag gttcggcccc gtcgacgaga tgggctgctt cgacctgtcc
     1741 gacggtggca tcgtcgaggt tgccgacccg accgggctgt tcctctcgcg gcaccctgac
     1801 ccgatgtcgg gtacgtgcgt gaccgtcacg ctggagggtc gcagaccgct gctcgccgag
     1861 gtgcaggcgc tcgtcacacc gactacggtt ccgtcgccgc gacgggcggt gagcggcatc
     1921 gagtccgctc ggctggcgat gctgctcgcc gtactgcagc gccgggtcgg tctcggcgta
     1981 cagtccgccg acgtgtacgc ctccactgtc ggtggcgcgg cgttgcgcga ccccgccact
     2041 gacctggccg tcgcgctggc cgtggcgagt gccacggtca acgtcgccgt cgacgagggc
     2101 acggtcgcca tcggcgaggt gggcttggca ggtgagcttc gcccggtgcc gaacatcggc
     2161 caacgactca ccgaggcggc ccggctcgga ttcacgtacg ccgtcgtacc aagccatcac
     2221 tcgtccccca gcgcccggtt gcccacgatc gacgcgatga cgctcatcga gtgtcctgac
     2281 ctgcaccggg cgctcaccgc cctcggcatc gctcaccagc cgccgcggcc gggacaggcc
     2341 gacctgcaga tcgtgtgagg ttcggccagc gctggcggga accgatcgcg ttcaccacga
     2401 tggagcaaga ccgacgacgc gtcggggccg ccgagggaga cagcgatgac cgttccgacc
     2461 agggacctgc tcgacgggac ccgacttccc gccatcgggt tcggcaccta cccgatgaag
     2521 ggcgcggaag ccgtcacggc gatcggcaac gcgatcgacg tcggctatcg gcttctcgac
     2581 acggccgtga actaccgcaa cgaagccgac gtcggtgagg cgatcaggcg gtctggcgtg
     2641 ccgcgcgagg agctgttcgt caccagcaag ctgcccggtc gccatcacgc gtacgacgac
     2701 gcgatcgcca gcacgagggg gtcgctgcag cgcctcggac tcgactacct cgacctgcac
     2761 ctcatccact ggccgaaccc gagcgtgggc aagtaccacg aggcctggcg ggctctggta
     2821 cagctccaga aggacggtct ggtgcgctcg atcggcgtct cgaacttcaa ccgcgaccac
     2881 ctcgagcgca tcatcgacga caccggcgtc accccgaccg tgaaccagat cgagctgcac
     2941 ccgtacttcc ctcaggagac gatgcggcgc gtcaacgccg accacgggat ccagaccgag
     3001 gcgtggagcc cgctcgggaa gcgatccgca cccttcacgg aaccggtggt tgccgggctc
     3061 gccgagaagt acggcgtcac gccgggccag gtcatcctgc gttggcacgt gcagcttggt
     3121 tcgatcccga tcccgaagtc ggccagtccg gaacggcaac gcacgaacat cgacgtgttc
     3181 ggtttcgagc tcgaggcggg cgaggtcgat gcgatcaccg gccttggccg gcgcaacggg
     3241 cgcctcttcg gcggcgaccc cgctcgtcac gaggagatgt agcccccggt cgactgggcc
     3301 cgcagctggg cgcggtggaa agtcgctcag cgggtgatcg tccaagccgt gctggacgtg
     3361 acgcccggcc ggtttgcagt ggctggtgaa catgcgcgtg cgccgcggcc cgagcgtgcc
     3421 tcgcggacac tctgtccgcc ttacactgat ggcgcaccag tcggcaacgg cggcaccgtc
     3481 tcactgacga accgtcactt cagggggtca agtggcaacc ggcgacaggc actctcccga
     3541 ggcgcggctc cgcgcgacgc tggcggcggt ggcccccggc accgagctgc gcgagggcct
     3601 cgaacgggtc ctgcgtgccc gcaccggcgc cttggtggtc ctcggcaacg atcgcaccgt
     3661 cgagtcgctg tgcacaggcg gcttcgccct cgagatcgac ttcacggcga cggcgttgcg
     3721 tgagctggcg aagatggacg gtgcgatcgt ctgtgaccgt gaagccaccc ggatcatccg
     3781 tgcgggtgtg cacctcatgc cggatgccgt catccagacg caggagaccg gtacgcggca
     3841 ccgcaccgct gaccgggtgg ctcgccagac cgggttcccg gtcatctcgg tctcgcagtc
     3901 gatggacctc atcgcgttgt acgtcggcga cacccgctac gtgctcgagg acaccggttc
     3961 catcctgtcc aaggccaacc aggcactcgc gacgctggag cggtacaagc tccggctcga
     4021 cgaggtctca gggacgctgt cggcgctcga gatcgaagac ctggtgaccg tgcgcgatgt
     4081 cgcctctgtg gcgcagcggc tcgagatggt gctgcggatc gccaaggaga tcgaggacta
     4141 cgtgctcgaa ctcggcaccg acggacggct actggcgctg cagctcgacg agctcgtcgc
     4201 cggcgtggac cacgagcgcg agttggtgat ccgggactac ttgccgaccg ggcgccgggc
     4261 tcgtacctct gactcggtgt tgaccgacgt ggacgcggtg accgctaccg agctgctcga
     4321 catcgcgacg gtcgcgcggg ccctgggtct cggcggcgcc gagagcctcg acagcgcggt
     4381 gagcccgcgc ggcttccggt tgctcgcgaa ggtgccccgt ctgccggggg cagtcgtcga
     4441 acgtctggtc gaccacttcg gcacgttgca gaagctgctg gcggcgagca tcgacgacct
     4501 gcaggcggtg gacggcgtcg gcgacagccg ggcccgctcc gtccgggagg gtctgtcccg
     4561 cctcgccgag tcgtccattc tcgagcgcta cgtctagctg cctggtgtgg gtttgcgatg
     4621 tttacagccc attcgggctg taaacatcgc aaacctcgga tggaaactgg cggcttgcgc
     4681 tgcacacccg gtgctgcatc ccgcgagccg gtccgctcag cgcttggctg gcttgtcgtc
     4741 ggtgatgtcg aagtacgcct tgtgcggctc gccaccgatc agcgccgcct ccagccagta
     4801 cccgcccggc tgcgggaggg cgtccaccgg ctgacagttc tccgccgaac gccttccgtt
     4861 ccatcggaac tcgtacgccg tcggcgggtc ggcccgcgcg acgacctgct tcaccgggag
     4921 tgagtcgggg cagtcgtcac tcgaccagac cacatcgggt ccggaggtga tccggaccgt
     4981 catggtgtcc ggcgtgatcg ccagcgtgca ggccggcgtg cgggtactgg tgaacaggat
     5041 cgtggcggtg ttggcctggc cgggcgccga gtccgcgaca tcgatcgcca tgaccacacc
     5101 cgctgggctg cagtcacccg tcggttcggc cagcgtggtc ttcggctgcg acttcggcgc
     5161 cttcgtgccg ctctccgttg cggtcgacga cgtggtggac gacgtggtgg acgacatggt
     5221 ggacgacgtg ctggatgacg acacgctgcc tggctgcgcc cctggttgat gcggccgctc
     5281 gccggggttg gtcgccgtcg tggtcgagac actgctcgtc ggcgtcgaag cgggtgagga
     5341 ggccggcgtc gacgacgtgg gcgaggatga cgtgcttgac gccggcggcg cagaggcgtt
     5401 cggtggggtc tcgttcgcgc tgccggagcc gctgaccagc caccacaatg acccggccag
     5461 gacggccacc acgacgagga gcacgagccg acgcgtccag tagacggcgg gcggcagcgg
     5521 tcccgtaggc cgcagcaccg cgttcatgcg ttccacgcta acgtggctgc gcggcgattc
     5581 gtggtaccgc gccgggcgcg cctccatgcg ccgatccggc agacatcggg aggacgaaca
     5641 gatgcccggg cgcgacattc cccttgtgca caagcggatc ctcgcgtggt acgacgacca
     5701 ccgccgcgcc ctgccgtggc gggacccgag cgccgagccg tgggcggtca tggtcagcga
     5761 ggtcatgctt cagcagaccc aggtggcgcg cgtgctcccg gtgtacgacg cgtggatgag
     5821 acgctggccc cgtccctccg ccctggctgc agaacccgca ggtgaggccg tacggatgtg
     5881 gggtcgtctc ggctacccgc ggcgggcgct tcggctgcac gccgctgctg tggcggtcga
     5941 cgcggagtac gacggcatcg ttccacgcac gtacgacgga ctgcgcgcac tccccggtgt
     6001 cggcgactac acggccaacg cggtgctcgc cttcgcgtac cggcagcgcg ccgtcgtcct
     6061 cgacaccaac gtccgtcgtg tgcacggccg tgtcttcgga ggcgtcccgt gcccacccag
     6121 agcggtcgcc gcggcagaac gccggaccgc ggctgcggtg ctgcccgatg aagccgagac
     6181 cgcagcccgc tggagcgttg cggtcatgga gctcggcgcc ttggtctgca cagcccgctc
     6241 acccatgtgt gcggtctgtc cggtcgccga tgcgtgttcc tggcgggccg cgggttaccc
     6301 gggccaggcg ggccgggtcc ggcgtgggca gtcgtacgcc ggaaccgacc gccagtgccg
     6361 aggccgcatc ctcggcgtac tcagggacag cgacgacccg gtcagccagg agtcggtggc
     6421 ggctgtctgg gatgacgccg atcagcgaag ccgcgccctg gcgtcactca tcgcagatgg
     6481 cctggcggtc gaacgtccag atggcctgct ctcgttgccg tcatgacgaa ggccccgtcc
     6541 tcgagggacg gggccttgcg tgtgtccggt gcgcgtgtct ggtcacgggc tgggcggcag
     6601 cgcctcctcg atcggcggcg cgtcgggcag ttcggacttc ggggtcccgg tgaaggagaa
     6661 ctgctgttca cccccgggat ccgtaccggc ctcgacgtcg acgagcacga tctggccggg
     6721 gccgacctcg ccgtacagca tcttctcggc gagcacgtcc tcgatgtcgc gctgcactgt
     6781 ccgccgcagc ggccgggcac ccagcacggg gtcgaagccg cgtctcgcga gcagttcctt
     6841 ggccgcctgc gtcagctcga gcgccatgtc gcggtccttc atccggatct c
//
LOCUS       DATBFZ010000315         7033 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_872253, whole genome shotgun sequence.
ACCESSION   DATBFZ010000315 DATBFZ010000000
VERSION     DATBFZ010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 7033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7033
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_872253"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1758)
                     /locus_tag="VH419_09720"
     CDS             complement(<1..1758)
                     /locus_tag="VH419_09720"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEY7043931.1"
                     /translation="MNDEQPPARRPGARKATDPAGPSRASGSATGDGRGTGTKPVGSG
                     KNGRVRMRDLSGWPKVRHIAKKTAKWGAIVGFAGLVTVAIAVYVTYRMIDIPNPNTDF
                     QAQTTTVYYSDGRHVLGQFALQNRESIPLSQMPKSLQYAAMSAEDRSFETNKGLDPKG
                     IARAAWNNLHSGSDTQGASTITQQYVKILYLSQERTWKRKVKEAFLAVKVQNTLSKDQ
                     IMEGYLNTIYFGRGAYGAQAAAQAYFDKDAADLTVPESAVLAAVLNSPGTLDPADGKD
                     NRQRLLERYRYVLDGMVSMGNLDEARAQRFEKHVPKLAVVEEKDTYGGQKGYLLTLVE
                     QQLKDEGFTDEEINGGGLKVVTTLDWKDQLAAQRAVNVVRPQGKPELHIALASVEPGT
                     GALRAMIGGRNYTGPGPQDQVNLATAASPAGSTFKPFALAAGLDDGYTLQSTFNGSSP
                     LELPDGSVVHNEGESSGIPNGISYGTVDLTKATEDSINTAYVDMTYKMKDGPEKILDA
                     AVAAGIPKTQDQSPGLTPNGRIALGTASIKPVEMAEAYATFADTGEHNDWFVIAKVTD
                     ANGLRYEHSDRPDRPLSRAV"
     gene            complement(1861..2274)
                     /locus_tag="VH419_09725"
     CDS             complement(1861..2274)
                     /locus_tag="VH419_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020574641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5318 family protein"
                     /protein_id="HEY7043932.1"
                     /translation="MWSQRSIIDYSLQRRATLATLSRGGGAAVEDVCDADPYTLRAAK
                     HHGEPAPVPCPVCKKPELAHLSYVFGEELGQYSGRIKSRAEIEVMAHEHGEIRIYVLE
                     VCRNCGWNHLVESYVVGDGVPRRPPRRKHTVEDDD"
     gene            2442..3218
                     /locus_tag="VH419_09730"
     CDS             2442..3218
                     /locus_tag="VH419_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020574640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEY7043933.1"
                     /translation="MSEIVLERPVRSDLPERTQQAQEREHMVAKRGDALELAVLGLLH
                     ESPMHGYELRKRVTGLLGWGRVLSYGSLYPCLKQMLRNGLIAEDTAHVDPAAPALAGK
                     RGKIVYKLTADGKDRFASLVSAAGPSAWEDDNFGVHFAFFGSATAETRIRIMEGRRSR
                     LEERLDRVRASVARSRERVDAYTLELQRHGLESVEREVRWLTELIDSERGKRTSRSAP
                     SVATGQHSTVGAAGADDTAYLPEQPSTSTDGTPSGDDNRE"
     gene            3229..4308
                     /locus_tag="VH419_09735"
     CDS             3229..4308
                     /locus_tag="VH419_09735"
                     /EC_number="5.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007079271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004512 - inositol-3-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006021 - inositol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HEY7043934.1"
                     /translation="MASVRVAIVGVGNCATSLIQGVHYYRDADPSGTVPGLMHVQFGD
                     YHVRDIEFVAAFDVDAKKVGFDLADATSASENNTIKICDVPPTGVVVQRGHTLDGLGK
                     YYRETIDESDAEPVDVVSVLRETEADVLVSYLPVGSEDADKFYAQCAIDAGVAFVNAL
                     PVFIASDPEWAKKFEDAGVPIVGDDIKSQVGATITHRVMAKLFQDRGVVLDRTYQLNV
                     GGNMDFKNMLERDRLESKKISKTQAVTSNIDHELGAKNVHIGPSDYVQWLDDRKWAYV
                     RLEGRAFGNVPLNLEYKLEVWDSPNSAGIIIDAIRAAKIAKDRGIGGPLLAASSYLMK
                     SPPVQRPDDEGRASVEAFIRGEVER"
     gene            complement(4490..4861)
                     /locus_tag="VH419_09740"
     CDS             complement(4490..4861)
                     /locus_tag="VH419_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011753656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY7043935.1"
                     /translation="MRLVQVAQHADDLDRAGEFYRALLGAAPTARFDPPGLVFFDLDG
                     VRLLLDRAAPSALVYLSVADVREAVEQLRATGVHVVSEPHVIFSHDDDLLGPAGTDEW
                     MAFVTDSEGNTIGLVSHLPRE"
     gene            4919..5401
                     /locus_tag="VH419_09745"
     CDS             4919..5401
                     /locus_tag="VH419_09745"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY7043936.1"
                     /translation="MVDHKGDVPSARETARALNSGAIHLLRSMRRVDRLAGLTPARLS
                     ALSVLVFAGPCSLGRLARAEDVAGPTMTRIVDGLEAAGLALRTEHPDSARSVLISATE
                     KGAQLMHEAADRRIEAIMSALQQMPARDRERIRAAAPALVELAEVVRGSEAGADGAPR
                     "
     gene            5398..6222
                     /locus_tag="VH419_09750"
     CDS             5398..6222
                     /locus_tag="VH419_09750"
                     /inference="COORDINATES: protein motif:HMM:NF025033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEY7043937.1"
                     /translation="MTTAVEITERLRLAGCVFPEREASLISQAFASAAERDEAVARRE
                     TGVPLEYVVGTAVFGDVTIAIGPPAFIPRARAVTLVDSADRLVDSADRLAGTEPGSRW
                     TVLDLGCGAGPIAAALRRRHPQWEVHACDHDTGALAWARRNGATFGFAVHEGDWFDAL
                     PRALEGKLDLVVAHLPYVPTTAVRLLPRDFRDHEPRHTVDGGADGLDPFRVVAAGCRR
                     WLAADGVLLTLVGQEQAATVLAFAADHRLGVELAPADEATGDSVVVSLRRSTGKPA"
     gene            6222..>7033
                     /locus_tag="VH419_09755"
     CDS             6222..>7033
                     /locus_tag="VH419_09755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY7043938.1"
                     /translation="MRFTAALRRLLAHQRFRQLFAVRLTSQAADGVIQVALASYILFS
                     PERQPDAASIAGALAAVLLPFSVLGPFVGVFLDRWSRRQVLLLSNLARLAPVLGAGLL
                     IAGGAGEAPLFAVVLLAFSINRFFLAALSAALPHVVDPDELVLANSVTPTSGTIAFMV
                     GLGAATGLRHVLPGQDPDAAVVMLAAAGYLTAALLALRLPRALLGPDLGMARAAVRED
                     LHNVVTGLIGGLRHLEARRPAAYALVVIASHRFFYGLSTVATILLYRNSFHP"
BASE COUNT         1049 a         2387 c         2367 g         1230 t
ORIGIN      
        1 gaccgcccgt gagagcggtc ggtccgggcg gtcggagtgc tcgtagcgga gaccgttcgc
       61 gtcggtgacc ttcgcgatga cgaaccagtc gttgtgttca ccggtgtcgg cgaacgttgc
      121 atatgcctcg gccatctcga ccggcttgat gctcgccgtc ccgagggcga tccggccgtt
      181 cggggtgagg cctggtgact ggtcctgggt cttggggata ccggcggcca cggcggcgtc
      241 gaggatcttc tccggcccgt ccttcatctt gtacgtcatg tcgacgtacg ccgtgttgat
      301 cgagtcctcg gtcgccttcg tcaggtcgac ggttccgtac gagatgccgt tcgggatacc
      361 ggagctctcc ccctcgttgt gcacgaccga gccgtccggc agctcgagtg gcgaggagcc
      421 gttgaacgtg ctctgcagcg tgtagccgtc gtcgagaccg gcggccaacg cgaacggttt
      481 gaacgtggag ccggccgggc tcgccgccgt cgccaggttg acctggtcct gtggtcccgg
      541 accggtgtag ttgcggccgc cgatcatcgc cctcaacgca cccgtccccg gctccaccga
      601 cgcgagcgcg atgtgcagct cgggcttccc ctgcgggcgg acgacattga cggcgcgttg
      661 tgctgcgagc tggtccttcc agtcgagcgt cgtgacgact ttcagcccac ccccgttgat
      721 ctcctcgtcg gtgaaccctt cgtccttcag ctgctgctcg acgagggtga ggagatagcc
      781 cttctgaccg ccgtaggtgt ccttctcctc gacgacggcg agcttcggca catgcttctc
      841 gaaccgctgt gcccgtgcct cgtccaggtt gcccatcgag accatgccgt cgagcacgta
      901 gcggtaccgc tcgagcaacc gctgccggtt gtccttgccg tcggccggat caagcgttcc
      961 cggagagttg aggactgctg cgagtacggc gctctccgga actgtcaggt ccgctgcgtc
     1021 cttgtcgaag tacgcctgcg cggcagcctg ggcgccgtac gccccgcgac cgaagtagat
     1081 cgtgttgagg tacccctcca tgatctggtc cttcgacagc gtgttctgca ccttgacggc
     1141 gaggaacgcc tccttgacct tgcgcttcca ggtgcgttcc tgcgacaggt acaggatctt
     1201 cacgtactgc tgggtgatcg tggacgcgcc ttgcgtgtcg ctgcccgagt gcaggttgtt
     1261 ccacgcggcg cgggcgatgc ctttcgggtc cagccccttg ttggtctcga aggaacggtc
     1321 ctccgccgac atcgcggcgt actgcaggga cttcggcatc tgggacaacg ggatgctctc
     1381 ccggttctgc agcgcgaact ggccgagcac gtgcctgccg tcggagtagt agaccgtcgt
     1441 ggtctgggcc tggaagtcgg tgttggggtt ggggatgtcg atcatccggt aggtgacgta
     1501 caccgcgatg gcgaccgtca cgaggccggc gaaaccgacg atcgctcccc acttggcggt
     1561 cttcttcgcg atgtggcgca ccttcggcca gcccgacagg tcgcgcatcc tgaccctgcc
     1621 gttcttgccg cttccgaccg gcttggttcc cgtacctcga ccgtcaccgg tggccgaccc
     1681 cgacgcccgg ctcgggccgg cggggtcggt cgccttgcgc gcgcccggcc ggcgggcagg
     1741 tggttgctcg tcattcacag gggaacactt ccgatttgct tcgatcgaac gggggggtgc
     1801 agggtcgcgg cctgtccggg tcagcccggg tctctcagat tcaggccggg tccggcaagg
     1861 tcaatcgtcg tcctcgacgg tgtgcttgcg gcgcggtggt ctgcgcggca ctccgtctcc
     1921 aacgacgtac gactcgacca ggtggttcca cccgcagttg cgacagacct cgaggacgta
     1981 gatgcggatc tcgccgtgct cgtgcgccat cacctcgatc tcggcgcgcg acttgatccg
     2041 gccggagtac tgaccgagct cctcaccgaa gacgtaggac aggtgcgcca actcgggctt
     2101 cttgcagaca gggcacggca cgggtgcggg ctcgccgtgg tgcttggcgg cgcgaagcgt
     2161 gtagggatcg gcgtcgcaga cgtcctcgac ggcggctccc ccaccgcggg aaagggtcgc
     2221 caacgttgct cgccgttgca gcgagtagtc gatgatcgag cgctgggacc acatggtgcc
     2281 atcgagggta cgagaaagac cacgtcggat tccaaccgcg ccgagacctc ccacaggcgg
     2341 ttctcaggat tcaggcttgc ggacacccta acgattcgat gtatcattcc gatacgtgat
     2401 gtcgataggt ccaaccgaga ggtggactcc atcgataccg gatgtccgag atcgtcctgg
     2461 agcgtcctgt tcgcagtgac cttccggagc gtacgcagca ggcacaggag cgggagcaca
     2521 tggtggccaa gcggggtgac gccctcgagc tggcagtcct cggactgttg cacgagtcac
     2581 ccatgcacgg ctacgagttg cgcaagcgtg tcaccggact gctcggttgg gggcgcgtgc
     2641 tgtcgtacgg ctcgctctac ccgtgcctca agcagatgct gcgcaacggt ctcatcgccg
     2701 aggacactgc gcacgtcgac ccagcggctc cggcgttggc cggcaagcgc ggcaagatcg
     2761 tctacaagct caccgccgac ggcaaggacc ggttcgcctc gctcgtctcg gccgccggcc
     2821 ccagcgcctg ggaggacgac aacttcggcg tccacttcgc gttcttcggc tccgcgactg
     2881 ccgagacccg catccggatc atggagggtc gccgcagtcg gctcgaggaa cgcctcgacc
     2941 gggtgcgcgc atcggtcgcc cgctcgcgcg agcgcgtcga cgcctacacg ttggagctgc
     3001 agcgacacgg cctggagtcg gtcgaacggg aggtccgttg gctgaccgag ctgatcgact
     3061 ccgagcgcgg gaagcggacg tcccggtccg ccccctccgt ggcgaccggc caacacagca
     3121 cagtcggtgc ggccggagcg gacgacaccg cctacctacc tgaacaacca tccaccagca
     3181 ccgacggaac cccgtccggt gacgacaacc gggaatgagg agagccgaat ggcttcggtt
     3241 cgtgtagcca tcgtgggcgt tggcaactgc gccacgtcgc tgatccaggg cgtccactac
     3301 taccgagacg ccgacccgtc ggggacggtc cccggactga tgcacgtcca gttcggtgac
     3361 taccacgtcc gcgacatcga gttcgttgcg gcattcgacg tcgacgcgaa gaaggtcgga
     3421 ttcgacctcg ccgatgcgac gtcggcgagc gagaacaaca cgatcaagat ctgtgacgtg
     3481 ccacccaccg gtgtggtcgt ccagcgcggc cacacgctcg acgggctcgg caagtactac
     3541 cgcgaaacga tcgacgagag cgatgccgag ccggtcgacg tggtctcggt gctgcgggag
     3601 accgaagccg acgtactcgt gtcgtacctt cccgtcggct ccgaggatgc cgacaagttc
     3661 tacgcgcaat gcgcgatcga cgcgggcgtg gccttcgtca acgcgctacc ggtgttcatc
     3721 gcttccgacc ctgagtgggc gaagaagttc gaggacgcgg gcgtcccgat cgtcggcgac
     3781 gacatcaagt cgcaggtcgg tgccacgatc acgcaccgcg tgatggccaa gctgttccag
     3841 gaccgcggcg tcgtcctgga ccggacctac cagctcaacg tcggcggcaa catggacttc
     3901 aagaacatgc tcgagcggga ccggctggag tcgaagaaga tctccaagac ccaggccgtg
     3961 acctcgaaca tcgatcatga gctcggcgcc aagaacgtcc acatcggccc gtccgactac
     4021 gtccaatggc tcgacgaccg caagtgggcc tacgtccgtc tcgagggtcg cgccttcggc
     4081 aacgtcccgc tcaacctgga gtacaagctc gaggtctggg actcgcccaa ctccgccggc
     4141 atcatcatcg acgcgatccg cgcggcgaag atcgccaagg accgcggtat cggcggcccg
     4201 ctgctggcgg cctcgtcgta cctgatgaag tctccgccgg tacagcgccc tgacgacgag
     4261 ggccgcgcct ccgtcgaggc cttcatccgt ggcgaggtcg agcgctagat ccttcgggtg
     4321 gttgagcctg tcgaaacccg ccccggtggt tgagcctgtc gaaacccgaa ccccctcgag
     4381 tcccgacaag ctcgatcagc gggtggcggc cagccccggt ggttgagcct gtcgaaaccc
     4441 ggacccctcg atctcgacaa gctcgatcag cgggtctgtc aggcgctggt cattcgcgtg
     4501 ggaggtggga gacgaggccg atggtgttgc cttcggagtc ggtgacgaac gccatccact
     4561 cgtcggtccc ggccggaccc agcaggtcgt cgtcgtggct gaagatcacg tgtggctcgc
     4621 tgacgacgtg cacgcccgtg gcgcgaagct gctcgaccgc ctcccggacg tctgccacgg
     4681 acaggtagac cagcgccgat ggggcagcgc gatcgagcag cagccgtacg ccgtcgaggt
     4741 cgaagaacac caggcctggc gggtcgaacc gcgccgtcgg cgccgctccc agcagggcgc
     4801 ggtagaactc accggcccgg tcgaggtcgt cggcgtgctg ggccacctgg accaggcgca
     4861 tgggggccac ctctctcgta gcatccctat cgattggtag ggatgctata catgaggggt
     4921 ggtcgaccac aagggggacg tgccgtctgc ccgcgagact gcgcgcgcgt tgaactccgg
     4981 tgccatccat ctgctccgca gcatgcgccg cgtcgaccgc ctcgccggcc tgacgccggc
     5041 ccgcctcagc gcgctgtccg ttctcgtctt cgcaggcccg tgctctctgg gcaggctggc
     5101 cagagccgag gatgtggcag gtccgacgat gacaagaatc gtcgacggac tggaggctgc
     5161 gggtctcgcg ttgcggacgg agcatccgga cagtgcgcgg tcggtcctga tctcggccac
     5221 cgagaagggc gctcagctga tgcacgaagc ggccgaccgg cggatcgaag ccatcatgtc
     5281 cgccctccag cagatgcctg cccgagatcg cgagcggata cgcgccgcag cgcccgccct
     5341 ggtcgagctc gccgaagtcg tccgtggatc cgaggccgga gccgacggag caccgagatg
     5401 accaccgccg tggagatcac cgagcggctt cgcctcgcag gatgtgtctt cccagaacgc
     5461 gaggcatccc tgatcagtca ggccttcgcg tctgccgccg agcgtgatga agcggtcgct
     5521 cgtcgcgaga ccggcgtacc gctcgagtac gtcgtcggca ccgccgtctt cggcgacgtc
     5581 acgatcgcca tcggcccgcc ggcgttcatt ccccgcgccc gcgccgtcac gctcgtcgac
     5641 tcggccgacc ggctcgtcga ctcggccgac cggctcgccg ggacggagcc gggatcccga
     5701 tggacggtgc tcgacctcgg ctgcggagcg gggcccatcg ccgctgcgct caggcgccgg
     5761 catccgcaat gggaggtgca cgcctgtgac cacgacacgg gggcactggc ctgggcgcgc
     5821 cggaacggtg ccacgttcgg cttcgccgtt cacgagggcg actggttcga cgcgcttccg
     5881 cgggcactcg aggggaagct cgacctcgtg gtcgcgcacc tgccgtacgt gcccacgacg
     5941 gcggttcgac tgctccctcg ggacttccgc gaccacgaac cccggcacac cgtcgacggg
     6001 ggcgcggacg gcctcgaccc gttccgcgtc gtcgcggccg gctgccgccg atggctggcg
     6061 gcggacggtg tcctcctgac cctcgttggg caggagcagg ctgcgacggt tctcgcgttc
     6121 gccgccgacc atcggctcgg ggtcgagctg gcacccgccg acgaagccac cggcgacagt
     6181 gtcgtcgtga gtcttcgacg ctcgaccgga aagcctgcgt gatgcgtttc accgcggcct
     6241 tgcgacgcct gctcgcccat cagcggttcc ggcagctgtt cgcggtccgg ctgacgagcc
     6301 aggcggccga cggggtcatc caggtggcgc tggcgtcgta catcctgttc tcgcccgagc
     6361 ggcaacccga cgccgcatcg atcgccggtg cgctggccgc ggtgctgctg ccgttcagcg
     6421 tgctcgggcc gttcgtcggt gtgttcctcg atcgatggag ccggcgccag gtgctgctcc
     6481 tttccaacct cgcgcgactg gctccggtgc tcggtgcggg gctgctcatc gccgggggtg
     6541 ccggcgaggc gccgctgttc gccgtcgtcc tgctggcgtt cagcatcaac aggttcttcc
     6601 ttgccgcact gtcggcagcg cttccccacg tggtcgatcc cgacgagctg gtgctcgcca
     6661 actccgtgac gccgacgagc ggcacgatcg ccttcatggt cggcctgggg gcggcgaccg
     6721 gcctgcgcca cgtgctgccg ggacaggacc cggacgccgc cgtggtcatg ctggcggcgg
     6781 ctgggtatct caccgcagcg ttgctcgcgc ttcggttgcc tcgcgcgttg ctcggccccg
     6841 acctcgggat ggcgcgggcc gccgttcgcg aggacctgca caatgtcgtg acgggactga
     6901 tcggcggact gcgtcacttg gaggcccgcc ggcctgcggc gtacgcgctg gtggtcatcg
     6961 cgtcgcaccg cttcttctac ggactgtcga cggtggcgac gatcctgctg taccgcaact
     7021 ccttccatcc cga
//
LOCUS       DATBFZ010000316         6749 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_2186576, whole genome shotgun sequence.
ACCESSION   DATBFZ010000316 DATBFZ010000000
VERSION     DATBFZ010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 6749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6749
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2186576"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="VH419_09760"
     CDS             complement(<1..315)
                     /locus_tag="VH419_09760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043939.1"
                     /translation="MGAFPETPTRGAEIEEREPVVVIIGSGAGGGTVAHELTARGIGC
                     VLLEAGPYLTAADYVNDEWAAFSQLAWLDMRTTSGSWRVATDFPNLPAWIVKAVGGST
                     THW"
     gene            complement(315..833)
                     /locus_tag="VH419_09765"
     CDS             complement(315..833)
                     /locus_tag="VH419_09765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043940.1"
                     /translation="MPETDATPPPADTSTETTLPAEIDADPGLSRHQVTILLRLLRVA
                     YPHPTFPDAPYERAAKSVQQADADHILARGLDDLDERAGGDFAGLNDVAATQLVEEVA
                     LSAFFKLVHATTVVALYDDHEVWDLLGYEGSSFDRGGYLDRGFDDLDWLPKPRIEEYD
                     GESRLELVEGDR"
     gene            977..1297
                     /locus_tag="VH419_09770"
     CDS             977..1297
                     /locus_tag="VH419_09770"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013861199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HEY7043941.1"
                     /translation="MYFIAVKFPTKPEWTDRWLDLVDDFTQATRNEPGNLFFEWSRSV
                     DEPNVFVLVEGFTDDGAGVHVNSAHFKQAMQDLRQGLAETPKIISKRVDGDGWDSMGE
                     LRID"
     gene            complement(1548..2669)
                     /locus_tag="VH419_09775"
     CDS             complement(1548..2669)
                     /locus_tag="VH419_09775"
                     /inference="COORDINATES: protein motif:HMM:NF018434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY7043942.1"
                     /translation="MRVVAAASGGEGHLGPLLPFVDAASRAGDEVVVVVPPEQEATVK
                     AQGVEYELTPGLAPAAVSAIRSAMASGGRAHRVRLAEVELFGRLCTEAALPTLGSVCG
                     RFRPALLLREPCDYASAIVSHRFGIPTAQVAISPGTADWDGLHLAAEVLEPIEPGVVE
                     TVEQAPYLSRFPHIDTPFPDCRAYGFTVGLDHREVQVPPLLWITFGTVNSAFDAQAGT
                     WQAVLRAVEDLPVEVVASVGRSGPSFASSGNTSIVDWIELPQVLAKASLVVCHGGSGT
                     TLAALSAGVPLVVIPLLADQLTNATMVEELGAGVAVRSNSDGPLRGLTLSDTARVRAA
                     INNVLLDDTYSAAARRAAGELAGRPTVSESMDRLREQFQ"
     gene            complement(2897..3853)
                     /locus_tag="VH419_09780"
     CDS             complement(2897..3853)
                     /locus_tag="VH419_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020577069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEY7043943.1"
                     /translation="MPTIDTAPTLRSITTAEGIADVVRRRYDMDVNACVLVRSFVNDV
                     YEIRTPRQRFVLKLYRHNGWSVDEVGWEAELVEHLFANGIPVSPVVPMTSGRAVGQLA
                     APEGVRPFLLSEYVEGRKPQEPFDDDLYRSYGRLIGRLQEAGDTFRTDRYRRPFGLEA
                     ILDEPLARVLPALTQQPADKRLAESLGVAARQRLTELADQLDWGVRHGDVTLDNVHRT
                     DAGLVIHDFDLAHVGWRVADLTGCLATPFADVFLTGYTEIRAVGTADREALPWLRVVE
                     CIRHLAFHLTDKPEWRGTESLGEGWVDKGLADLRGAAQRLVS"
     gene            complement(4223..4963)
                     /locus_tag="VH419_09785"
     CDS             complement(4223..4963)
                     /locus_tag="VH419_09785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043944.1"
                     /translation="MRTTLAAAAACSLIATGAIITLTPATADAASCTRPTIRSTTIDP
                     STVVLGTSVPKGITVTTKIETNGCRIDRVEAGLYGPNFIDSYGLDRVDTSNGATTYET
                     GLRISPGGLPNADAGRWTSYVTAWGPKTAEGPGPSFRVLRAARLTTNATPEPVRKGAK
                     LTIKGSLTRANWESLTYQGYGNRVVDLQRRSAGGSYRTVKHVTSAANGALRTTVTATA
                     DACYRFVFAGSANTAPTTSKGDCVDVRS"
     gene            5309..5494
                     /locus_tag="VH419_09790"
     CDS             5309..5494
                     /locus_tag="VH419_09790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043945.1"
                     /translation="MARSIRIGDVSEFVDAVRRVAHGGSAIDPTVVSALLRRRRSGDP
                     LARLTPREREVLELIAI"
     gene            complement(5883..6245)
                     /locus_tag="VH419_09795"
     CDS             complement(5883..6245)
                     /locus_tag="VH419_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012923223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY7043946.1"
                     /translation="MTLGWYTLVIDAADPHRLAAFWAEVLGWPTAYTVQDEVALEAPN
                     DPDDRVPALLFVRDQGAKAQKNRLHLDLNPDDQAHEVQRLLDLGARRVDIGQRNANWV
                     VLADPEGNEFCVLSPRTS"
     gene            complement(6242..6640)
                     /locus_tag="VH419_09800"
     CDS             complement(6242..6640)
                     /locus_tag="VH419_09800"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEY7043947.1"
                     /translation="MLAEEAIWEAGLWGIDPHRRHAHSGVVPARTRRGRGLGRDVVQV
                     PCDYAFRPRGLHQLQADTRALNTPMRAAATAAGFQQEGVLHEAGWHDGAFIQTLVHGL
                     LAPEWIGSTAKQHRSPVTDRPDVPPVEIRP"
BASE COUNT         1101 a         2209 c         2327 g         1112 t
ORIGIN      
        1 ccagtgggtg gtcgagccgc cgaccgcctt cacgatccac gccggcaggt tcgggaagtc
       61 ggtggccacc cgccacgatc cgctcgtcgt gcgcatgtcc agccacgcca gctgcgagaa
      121 ggcggcccac tcgtcgttca cgtagtcggc ggcggtgagg tacggcccgg cctcgagcag
      181 cacgcatccg atgccgcggg cagtcagctc atgggcgact gtcccgccgc cggcgccgga
      241 gccgatgatg acgactaccg gctcccgctc ctcgatctcg gcaccgcggg tcggcgtctc
      301 ggggaaggcg cccatcaccg gtcaccctcg accagttcaa gccgtgattc gccgtcgtac
      361 tcctcgatcc gcggcttggg cagccagtcg aggtcgtcga agccgcggtc gagatagccg
      421 ccgcggtcga acgaactgcc ttcgtagccg aggaggtccc acacctcgtg gtcgtcgtac
      481 agcgccacaa cggtggtcgc gtggaccagc ttgaagaagg ccgagagcgc tacctcctcg
      541 acaagctgtg tcgccgcgac gtcgttgagg ccggcgaaat cgcccccggc acgttcgtcg
      601 aggtcgtcga gcccgcgagc caggatatgg tcggcgtccg cctgctgcac ggatttcgcc
      661 gcccgctcgt acggcgcatc cgggaaggtg ggatggggat aggcgacccg aagcagccgc
      721 agcaggatcg tcacctggtg cctggacaga cccggatcgg catcgatctc cgccggcaga
      781 gtcgtctcgg tactggtgtc ggccggcggg ggagtcgcat cagtctcggg cacgctgcct
      841 ccttgttgac gatccgatgt ggacgatgcg cctggagctc cgaccacagt cgccccgggc
      901 tgtggcccgg gtcacagaca gtactatcgc gatcattctg ggtcttgtgc ccttacggca
      961 agcaaaggat ctgccgatgt acttcatcgc cgtcaagttc cccaccaaac cagagtggac
     1021 cgaccgctgg ctggacctgg tcgacgactt cacgcaagcc actcgcaacg agccgggcaa
     1081 cctcttcttc gagtggtcgc gcagcgttga tgagcccaac gtgttcgtgc tcgtcgaagg
     1141 cttcactgac gacggtgccg gggtgcacgt caacagcgcc cacttcaagc aggcgatgca
     1201 ggatcttcgc caaggacttg ccgagacgcc gaagatcatc agcaaacggg tcgacggcga
     1261 cggctgggac tcgatggggg aactgcgcat cgactgatgt ccgattccgg ccccggcggc
     1321 tggcgacggc ccgccgatcg ccgaccggcg tcagatctgg gtcgggctgt tggttcgcag
     1381 agtcgaatcg gcgcaacgca ggggaagtta cgcgctcggg tgatggcaac ctttgacagg
     1441 cctgtccaga ttgcgtcgcg tttgtgatgc tcgcgcaact tccctcgatc gtgatgaact
     1501 gctgcagaag ggctggtgaa acgaagcagg agcgcggaat cggtgctcta ctggaactgt
     1561 tctcggagtc ggtccatcga ctctgacacc gtcggtcgac cagcgagttc gccggcagcg
     1621 cgccgcgcgg ccgcgctgta ggtgtcgtcc aagagcacgt tgttgattgc tgcccgcacc
     1681 cgtgcggtgt cgctcagtgt gagtccacgc agcgggccgt cggagttcga acggactgcc
     1741 acgccggcac cgagttcctc aaccatcgtc gcattggtca actggtccgc cagcagtgga
     1801 atgacgacca gcggaacgcc ggctgacagg gccgcaaggg tggtgccgga accgccgtga
     1861 cagacgacaa ggctggcctt ggcgagtacc tgcggaagct cgatccagtc gacgatcgag
     1921 gtgttgcctg aggaggcgaa actcgggccg gagcggccca cagacgcgac cacctcaacc
     1981 gggagatcct cgaccgcacg caagacggcc tgccatgtgc ccgcttgcgc gtcgaacgcg
     2041 gagttgacgg ttccgaacgt gatccagagc agtggcggga cctgcacctc ccgatgatcg
     2101 agtccgacgg tgaacccgta ggcgcggcag tcggggaacg gcgtgtcgat gtgggggaag
     2161 cggctcaggt acggggcctg ctccaccgtc tcaacgacgc ccggctcgat cggctcgaga
     2221 acctcggcag ccaggtgcag accgtcccag tcggcagttc cgggggagat cgcgacctgt
     2281 gccgttggga tgccgaatcg atgcgaaacg atggccgagg catagtcaca aggctcacgg
     2341 aggagcaagg ccggacggaa ccggccacag accgacccga gcgtcggaag agccgcctcg
     2401 gtgcacaacc gcccgaagag ttcgacctca gcaagcctca cccggtgagc acgaccacca
     2461 ctggccattg ccgaacggat cgctgagact gcggccggag ccagtccggg cgtcagctcg
     2521 tactcaaccc cttgtgcctt gactgtcgcc tcctgctctg gaggcactac cacgacgacc
     2581 tcgtcgccgg cccgcgaagc cgcgtccacg aacggaagca atggaccgag gtggccctcg
     2641 ccgcctgagg ctgccgccac gacgcgcacc tggagagcct aacgaggttc tcgcgcgatt
     2701 tgtcgccgac gacggagctc ggaccagagc aacgacaaca tgtccagacg ttgccgtttc
     2761 tcgccatctt gtcggtttcc tcagggctcg attgtctgcg agtgttcgga taggacggat
     2821 cggtgtgtgc cgttgagggg accgtcgtgc ggaggatgcg ttggtcgacc ctcctgcggg
     2881 gatggcgctc ggggcgctac gagaccagcc gctgagccgc ccctcgaagg tcggccaagc
     2941 ctttgtcgac ccacccctcg cccagcgact cggtacctcg ccactccggc ttgtccgtca
     3001 ggtgaaacgc cagatgtctg atgcactcga cgacgcgaag ccacggcagg gcctcccggt
     3061 cagccgtccc gaccgcacgg atctccgtgt agccggtgag gaacacatcc gcgaaggggg
     3121 tcgcgaggca gccggtgagg tcggccaccc gccatccgac gtgggccaga tcgaagtcgt
     3181 ggatgacgag cccagcgtca gtgcggtgca cgttgtccaa ggtgacatcg ccatgacgga
     3241 cgccccagtc cagctggtcg gccaactcgg tcagccgctg tcgcgcggcg acgcccaggc
     3301 tctcggccag gcgcttgtcc gcgggttgct gcgtcagcgc tgggagcacc cgggcgagcg
     3361 gctcgtcgag gatcgcctcc agaccgaagg gacgccgata tcgatcggtg cggaaggtgt
     3421 ccccagcctc ctggagccgc ccgatcagac ggccgtacga gcggtagagg tcatcatcga
     3481 acggctcctg gggcttgcgg ccctcgacgt actccgacag caggaacggg cgcacccctt
     3541 ctggtgcagc gagctggccc accgcccggc ccgaggtcat cggtacgact ggcgacacgg
     3601 ggatgccgtt ggcgaaaagg tgctcgacca gctcggcctc ccaaccgact tcgtcgacgc
     3661 tccaaccgtt gtggcgatac agcttcagca cgaagcgctg ccgaggcgtg cggatctcgt
     3721 acacgtcatt gacgaaggac cgcaccaaga cgcaagcgtt gacatccatg tcgtagcggc
     3781 ggcgcacgac gtcagcgatg ccctccgccg tcgtaatcga gcgcagcgtc ggagctgtgt
     3841 caatggttgg catttggcga gagtacgaga cacacggcgg agcagcacgg aagtttcccg
     3901 gtgcgcgcag acgctgccgc atccgaacgc gcacctcgaa ggtgagcgtg tgcgcgaccg
     3961 gtccgtctcg caagccgtcc gtcaactgcg aacgagtgcc gacgagatcg ccggtgccgt
     4021 cgtccacctg gtcggccgag cgtctctgat cgaccaccag cacctcgacg gtgggcggcg
     4081 gcatgatcga cctctgcctg cacacctgac gcgtgcggca gcaccgtctc gaccgcatgc
     4141 ccgcgataag cggcggccac gtcgttggct cacctacacc tgatcacgct cgacaaggtc
     4201 ccacattcgg ggaccaggcc gactacgagc ggacgtcgac gcagtcgccc ttcgacgtgg
     4261 tgggtgcggt gttcgcgctg ccggcgaaca cgaaccggta gcaggcgtcc gcggtcgccg
     4321 tcactgtcgt ccgcagcgcg ccgttggcgg cgctggtcac gtgcttcacg gtgcggtagg
     4381 acccgcccgc agaccggcgc tgcaggtcga cgacccggtt gccgtagccc tggtaggtca
     4441 gcgactccca gttggcacgg gtcagcgaac ccttgatcgt tagcttggcg cccttgcgga
     4501 cgggctcggg cgtggcgttg gtggtgagcc tggccgcgcg caggacacgg aagctgggac
     4561 cgggaccttc ggccgtcttc gggccccacg ccgtgacgta cgacgtccat cggccggcgt
     4621 cggcgttcgg caggccaccg gggctgattc gcaagcctgt ctcgtaggtc gttgcgccgt
     4681 tgctggtatc gaccctgtcg aggccgtagc tgtcgatgaa gttggggccg tagagcccgg
     4741 cctcgacccg gtcgatccgg cacccattgg tctcgatctt ggtggtgacg gtgatgccct
     4801 tgggtaccga cgtgccgagc acgacggtcg atgggtcgat tgtggtgctc ctgatagtgg
     4861 gcctggtgca gctagcggcg tcggcagtgg ccggggtgag cgtgatgatc gccccggtgg
     4921 cgatcaagga gcaggcagcg gcggcggcca gggtggtgcg catcgtggtt cctctcggtg
     4981 gcgcgcccag tggggctcgc cgtcgtggtg gttgatgggc ccacgatctc cggccccggt
     5041 tggggtggcc ttgggatcgg cttggggacc gaggccgcct gctgacttcc ggacggttgg
     5101 tcgccggtca cgacgagtcc agaaatccct tccggtcaag acgaggaaga ggaggcacag
     5161 cagggcctcc acgatgcctc gggcctgcca cacccacgcc gtgtccagcc gccaggtcag
     5221 cgggacggtg gcgttgaagg atgcgtgcat caagccggcg agcagcacac tcgacgtacg
     5281 tagatagatc cagctcagga ggacggacgt ggcgagaagc atccgcatcg gcgacgtcag
     5341 cgaattcgtc gacgccgtac gccgcgtggc ccacggcgga tcggcgatcg accccacggt
     5401 cgtctcggct ctgctccggc gacgccgcag cggcgatccg ctggcgcgcc tgactccgcg
     5461 cgaacgggag gtgctcgaac tgattgcgat ttgacctgcg ggcggttgcg gatgaagcac
     5521 ttcggccact gctgcctggt gacgaccggg acagttcagc cgacgtagtg ctgccggcgg
     5581 cctgttggca ggtcggggtc gatcctcgca gcccccgttc gtgctccgcc cgcgtcccag
     5641 gatacgacta ccgtgttcgc ccagtcccgc gccagcggcc ggaaagcgac cacgtgctgc
     5701 ggggtttcgt tgatgaaact gcggaacggc ttccggtccg tgctggggcc agaagccaga
     5761 tcagtagtca tgggtgttgc ggccgagcag gatcgtgtct cgcatatcga ggacccgggc
     5821 caggttggcg ctgacctcct cgccgccgtc ggacctccag tcgcccggct cgtccagcga
     5881 ggctaggagg tccgggggga caggacgcag aattcatttc cctcggggtc ggcgagcaca
     5941 acccagttgg cgttgcgctg gccgatgtcg acgcggcggg caccgaggtc gaggagacgt
     6001 tgcacctcgt gcgcttggtc gtcggggttg aggtcgaggt gcagccgatt cttctgagcc
     6061 ttggcgcctt ggtcccggac gaacagcagc gccgggacgc ggtcgtccgg gtcgttgggc
     6121 gcctcgagcg ccacttcgtc ctgcacggtg taggcggtgg gccacccgag cacttcagcc
     6181 cagaacgcgg cgagccggtg ggggtcggcg gcatcgatga ccagcgtgta ccagccaagc
     6241 gtcatggccg gatctccact ggtgggacat cgggtcgatc cgtgacgggg gagcggtgct
     6301 gcttggctgt tgatccgatc cactcggggg caagcaggcc gtgcacgagg gtctggatga
     6361 aggcgccgtc gtgccagcct gcctcatgca gcacaccctc ctgctggaag ccggccgcgg
     6421 tggcggcggc tcgcatcggg gtgttcagcg ctcgtgtgtc ggcctgcagc tggtgcagcc
     6481 cccgtgggcg gaaggcgtag tcgcagggca cctgcacgac gtctcggccc agcccacggc
     6541 cgcgtcgcgt ccgggcgggc actaccccgg agtgagcgtg gcggcggtgc ggatcgatgc
     6601 cccacagccc ggcttcccag atcgcctcct cagccagcat ctggcacccc ttcgacgggc
     6661 caattccacc gcatcgaccg ggaagcgcac catcgagctc tctcgaagat ctccggcgcc
     6721 gtgcagacgt cggccgattc gtcgtgcgg
//
LOCUS       DATBFZ010000317        14856 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_1855894, whole genome shotgun sequence.
ACCESSION   DATBFZ010000317 DATBFZ010000000
VERSION     DATBFZ010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 14856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14856
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1855894"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="VH419_09805"
     CDS             <1..411
                     /locus_tag="VH419_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEY7043948.1"
                     /translation="AVVQGHPDPLVGTIDAPIVRHQRHDHKFTVGHGGRHSITHYETL
                     EAHRFASLLEITLETGRTHQIRVHMSALHHPCVGDLTYGADPVLANRVGLGRQWLHAV
                     RLGFEHPERERWVEFESEYPEDLAHALDVIRNAH"
     gene            401..862
                     /locus_tag="VH419_09810"
     CDS             401..862
                     /locus_tag="VH419_09810"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY7043949.1"
                     /translation="MPTDDRQVRRATPSDSDAIADLYTDIRDENLGPIPPPVHSREEN
                     HDWVRHILLPTHEVWVAERRGELIGFMALHRPDWLGHLYIRASATGHGLGSRFVELAK
                     RELPGLIQLWTFQSNAGARRFYERHGFVAVQWTDGDNEEGAPDVRYELRRD"
     gene            1029..1493
                     /locus_tag="VH419_09815"
     CDS             1029..1493
                     /locus_tag="VH419_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009073438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY7043950.1"
                     /translation="MATGESRREGQMDWTLEVVVLPVSDLARSIEFYRDRVGFTLDHE
                     TKNQHMHVAQLTPPGSGCSIVIGDLPSQNQMEPGSMRGLQLCVADAETARGELLSRGV
                     EASEITVFDERDGGTFFGFADPDGNTWAVQQLKARATTPLIPPEHRSRFGAE"
     gene            1735..5253
                     /gene="dnaE"
                     /locus_tag="VH419_09820"
     CDS             1735..5253
                     /gene="dnaE"
                     /locus_tag="VH419_09820"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEY7043951.1"
                     /translation="MPDSFVHLHAHTEFSMLDGAARVDELLDETVRLGMPALAITDHG
                     NMFGAFEFHKAATSRGVKPIIGIEAYLTPGTSRFEKRRVQWGNGGEDDVSGGGAYTHL
                     TMLAETTEGMHNLFRLSSLASIEGQYYKPRMDRELLERYGAGVIATTGCPGGEVQTLI
                     RLGKYADAVQSAADYRDIFGSGSYFVELMDHGLPIERRVRSELLQIAADLSLPVVATN
                     DLHYTHKDDAVGHEALLCVQSGSTMNDANRFKLEGDGYYLKSPAEMREVWRDLPEACD
                     NTLLIAERCDVAFAEGRNLMPRFPVPDGETEETLFVKQVERGLAARYPDGVTREVRAR
                     ADREIAIILQMGFPGYFLVVADFINWAKAQGIRVGPGRGSATGAIVAYALGITELDPL
                     VHGLLFERFLNPERIDMPDIDIDFDERRRGEVIRYCTEKYGDDRVAQIVTYGTIKAKQ
                     AIKDSSRVLGYPFAMGERITKAMPPPVMGKDIPLSGVFDPDHSRHGEAGEFRSLYDAD
                     PDVQRIVDTARQLENLKRQWGVHAAGVIMSSEPLIDVVPIMRREQDGATITQFDYPAC
                     ETLGLLKMDFLGLRNLTVIDDTLANIVANRDVTIDLDNLGLDDAATYELLSSGDTLGV
                     FQLDGAPMRALLRQMKPDSFEDIAAVLALYRPGPMGANSHTNYALRKNGKQEIKPIHP
                     ELEEPLKEILAPTYGLIVYQEQVMEIPQKLAGYSLGKADLLRRAMGKKKRAVLEAEFE
                     GFRDGMLANGYSPGAAKTLWDILVPFSDYAFNKSHTAGYGLVSYWTAYLKANYPSEFM
                     AALLTSVRGDKDKSAVYLNECRRMSIKVLPPDVNESEADFTPVGKDVRFGLSAIRNVG
                     NNVVSGLLGARESEGKFTDLNDFLDKVPAVVCNKRILESLCKAGAFDSLGYTRRGLVA
                     IHEEAADYYVDLKKNEEFGQDSLFAGLDDVGFSAGSVEVPDLAEWDKKTLLAFERDML
                     GLYVSDHPLNGLEHVLASTSDVTIGQLITDDSREEGAVVTVAGLITSVVRKTTKKGDL
                     YAVVTVEDLEGAINVMVFPRDYQLSSTLLVEDAIVLVKGKVKYSRDEALELTALEVSV
                     PDLTKGITNGPVVISMPAVRVTPPIVDQLKDVLATHPGVAEVHLRLQSQGRTMLMRLD
                     ERLRVTPSPALMADLKALLGPSCLSS"
     gene            5351..6016
                     /locus_tag="VH419_09825"
     CDS             5351..6016
                     /locus_tag="VH419_09825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043952.1"
                     /translation="MSSAGPPSSTTMSSSPEPMPPVASSPQGMFETFEDPPSSRLERL
                     RFVSVCLVVGVLAAVPAAWLWVELADPPSTRLSSDGLVFGELAFNDVTSITLWFVVVG
                     FAWGVVLGLVATSLGRRHGLVTVAAVLLMSVVGSTVMLWCGIHLFGPDHPVDFVALFN
                     AEPQERRQMLDGFHSGDLLVSSLRLTTWVALLAWPIGAMAGALGGTYLWPKAPKPPQM
                     PPS"
     gene            6075..7886
                     /locus_tag="VH419_09830"
     CDS             6075..7886
                     /locus_tag="VH419_09830"
                     /inference="COORDINATES: protein motif:HMM:NF023260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3352 domain-containing protein"
                     /protein_id="HEY7043953.1"
                     /translation="MSSNTPPPGSGGPPWGQPQQPGQGWGQQPPGGQQPGGYGQPGPS
                     GQPPAPGQPGPYGQPGQYGQPGQPGPYGQPGQPGPFEQASGPGGPGTGEPQGGSGGAR
                     RGLTALIVAVVVVVLAAGIAVAIPFIKNKLSSAGPQAAEAIPADAVFYVGVDLDPSAE
                     QKINALRFLNHFPAFKEETGVTDANTDVRKTIFDKLLESEPCPGVTYDDDIKSWIGSK
                     FAFAGMPPASGSSEPVVLGAVEVTDQSEAKTGIRKLAECGSDPSTFGLAFTGDYAVIA
                     ETQDEADKYADAASSASLADDGDFSADMDELGDLGVATSWVDIKGAIDLFGSDISAED
                     DEAGLDFIRSAYQRAAMTFRFESDHAEFASAVYGDLPDVDHGTNQIVNLPDSTVLAMS
                     EAGGGNRIAAQWDDLMEVAKSSGVDVDQEIQKFEDRTGLVLPDDLETVLGDNLLLAVD
                     GDGLTADALNSGDVAQINAGVRFSGDKAELDRLYQKVLDLIEEETGTQAETVKKDTDD
                     GFVLATNDSYADKLASGGDLGDSDAFTSVVDNGADQEFVLFFNFDQVENQILQAAQDQ
                     GASAEALDNLRPLQAVAITSDTDGKYAHGTLVISVND"
     gene            complement(8313..8993)
                     /locus_tag="VH419_09835"
     CDS             complement(8313..8993)
                     /locus_tag="VH419_09835"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEY7043954.1"
                     /translation="MGEPLPLFPLNTVLFPGVVMPLHIFEDRYRMLVKDLIALPPDAA
                     REFGVVAIKVGYEVGRRGVHTIQRTGCAALVTEVTANPDGTFEIMVIGRRRFHVESLD
                     PSQDYLRADVEWLPDASGGTSPADAREAAEQAASLFAVYREKVARLRGDDILEGPAPT
                     SPVDLSYTLAAALVLNLAERQAMLEAPDVVSRLRLGTSIMRAELQAVAAIPSLPATSM
                     ARTAWSPN"
     gene            9083..10420
                     /gene="hisD"
                     /locus_tag="VH419_09840"
     CDS             9083..10420
                     /gene="hisD"
                     /locus_tag="VH419_09840"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEY7043955.1"
                     /translation="MIRRIDVRGRAATGEASDYRSLVPRAAFDVEAAMERVRPLCEAV
                     RRDGHAELVRQAERFDGVRIGSVRVPGGALHDALDGLDPSLRASLEESVRRLRTTCEA
                     ELPADVTTVVTGGGTVTQRNVPVRRVGLYVPAAKAPLISSVVMNVVPAQVAGVWSLAL
                     ASPAQRDHDGLPHPSILAACAVLGVDEVYAAGGAGAIAMFAYGYQPDQSTARSDPGCV
                     PVDLVTGPANIYGVAAKRLLRGVVGIDAEAGPTEIAILADESADPTFVAADLISQAEH
                     DPMAASVLVTPSLRLVEAVEDELARQVAATKHTERVHEALSGQQSGIVIVEDLEQGED
                     VVNAYAAEHLEIHTVDAPARAGRIMNAGAIFVGPYAPVSLGDYAAGSNHVLPTAACAC
                     HSSGLSVRSFLKTVQVVDYTAQALADVAGPVTTLAEAEDLPAHGEAVRIRLER"
     gene            10420..11514
                     /locus_tag="VH419_09845"
     CDS             10420..11514
                     /locus_tag="VH419_09845"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEY7043956.1"
                     /translation="MTGDDLPVRPELAGIEPYGAPQLEVSVRLNVNENPYPPPPHVVE
                     AMAAAVRDAATTLNRYPDREARALRTALADYLGHGLTAEQVWVGNGSNEVMLHVLQAF
                     GGAGRTVLSFAPTYSMYPEYARDTSTRWVVGRRTDDFGIDIDHAVNLIGQAQPHVVLL
                     ASPNNPTGTALPLDAVKAICTVARGVVVVDEAYAEFRRSGTPSALSLLDDFPRLAVTR
                     TMSKAFALAGGRVGYLAASRDFVDALRVVRLPYHLSATTQAVARVALEHRAELLARVA
                     DLRAERDATAAWLRRQGLDVADSDANFVLFGTFGDRHAVWKDLLDHGVLIRETGPEGW
                     LRVSIGTAAEMDAFRQALQATLSSLEEGSS"
     gene            11511..12113
                     /gene="hisB"
                     /locus_tag="VH419_09850"
     CDS             11511..12113
                     /gene="hisB"
                     /locus_tag="VH419_09850"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008362999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HEY7043957.1"
                     /translation="MTTRAARIDRETNESKVVVELDLDGSGRAEVSTGVGFYDHMLTS
                     LARHALWDLTVQAQGDLHVDAHHTVEDVAIVLGEAMRTALGDKAGIRRFGDALVPLDE
                     ALVQCAVDVSGRPYCVQTGEPAGQAYAVIGDGYAGSLTRHVFESMSAHASIAIHVRVL
                     AGRDPHHIVEAQFKALARALRDAVAHDPREPGVPSTKGHL"
     gene            12110..12754
                     /gene="hisH"
                     /locus_tag="VH419_09855"
     CDS             12110..12754
                     /gene="hisH"
                     /locus_tag="VH419_09855"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015303108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEY7043958.1"
                     /translation="MTAGARPRVVLLDYGSGNLRSAERALTRAGADVTIDADHATALG
                     ADGLVVPGVGAFAACMAGLTAVRGPEIIGRRLAGGRPVLGICVGHQVLFEQGIEHGQP
                     TAGCGEWPGRVERLHAAVLPHIGWNTVEAPPESAMFAGVEQERFYFVHSYAARAWTLV
                     TNGRTRAPLVTWTTHETDRFVSAVENGPLWATQFHPEKSGDAGAVLLRNWVQQL"
     gene            12751..13203
                     /locus_tag="VH419_09860"
     CDS             12751..13203
                     /locus_tag="VH419_09860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043959.1"
                     /translation="MNRRIAAALTLALLTFTTACGSSESGGESSGGDPTSTSSDATND
                     ADPSTDAVSVMFHKSGGLRPVDERTFYSADAPPPAGKTRADVDDVLDAASNPDLRDVD
                     AAPMPENVCCDRQEYMVTISYADGTSVTYRTIDGLQQPVVLERFLGML"
     gene            13211..13939
                     /gene="priA"
                     /locus_tag="VH419_09865"
     CDS             13211..13939
                     /gene="priA"
                     /locus_tag="VH419_09865"
                     /EC_number="5.3.1.16"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007078048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     1-(5-phosphoribosyl)-5-((5-
                     phosphoribosylamino)methylideneamino)imidazole-4-
                     carboxamide isomerase/phosphoribosylanthranilate isomerase
                     PriA"
                     /protein_id="HEY7043960.1"
                     /translation="MTTLELLPAVDVSGGRAVQLVQGVAGSERVFGDPVAAALRWQQA
                     GAQWIHLVDLDAAFGRGDNRSLLREVVAALEIDVELSGGIRDDESLAAALSTGCRRVN
                     IGTAALEDPAWCARVIAAHGDRVAIGLDVRGTTLAARGWTRDGGHLHEVLARLDQAGC
                     ARYVVTDVKKDGMMQGPNLDLLRDVCARTQAPVVASGGVSTIDDLVAISGLVDDGVEG
                     AIIGTALYTGAFTLEDALAAVDKG"
     gene            13936..14697
                     /gene="hisF"
                     /locus_tag="VH419_09870"
     CDS             13936..14697
                     /gene="hisF"
                     /locus_tag="VH419_09870"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEY7043961.1"
                     /translation="MTVAVRVIACLDVDDGRVVKGVRFADIRDVGDPVELASRYDEQG
                     ADELTFLDITASQQRRDTVLDVVRRTAEEVFIPLTVGGGVRTVDDVDVLLRAGADKVA
                     INTGAIERPEAISEIAQRFGRQVLVLSVDARRDSQTRSGFEVTTHGGSQGSGLDAVEW
                     ARAGAEHGAGEILLNAMDADGTQQGFDLELIGAIRAVVTIPVIASGGAGVAEHFPPAI
                     EAGADAVLAASVFHDRLLTVADVKRALAATGHPVR"
BASE COUNT         2443 a         5062 c         5058 g         2293 t
ORIGIN      
        1 gccgtcgtgc aggggcaccc cgatccgctg gtcggcacga tcgacgcgcc gatcgtccgg
       61 caccagcggc acgaccacaa gttcacggtc ggacacggcg gacggcacag catcacccac
      121 tacgagacgc tcgaggcgca ccggttcgcc tccttgctcg agatcaccct cgagacgggc
      181 cgcacgcacc agatccgcgt gcacatgagc gcattgcacc atccgtgcgt cggcgacctg
      241 acgtacggcg ccgaccccgt cctcgccaac cgcgtcggac tcgggcgtca gtggctgcat
      301 gccgtacgac tcggcttcga gcatccggag cgggaacgct gggtcgagtt cgagtccgag
      361 tacccggagg acctcgccca tgccctggac gtcatccgaa atgcccactg acgaccgcca
      421 ggtccgacga gcgacgccgt ccgattcgga cgcgatcgcc gacctctaca ccgacatccg
      481 cgacgagaac ctcggtccga tcccgccacc ggtccactcg cgggaggaga accacgactg
      541 ggtgcggcac atcctgttgc cgacgcacga ggtgtgggtg gcggaacggc ggggggagct
      601 gatcgggttc atggcactgc atcgcccgga ctggctcggt cacctctaca tccgtgccag
      661 tgccaccggc catggtctcg gcagccggtt cgtcgagctg gccaagcgtg agctgcccgg
      721 cctcatccag ctctggacct tccagagcaa cgccggtgcg cgtcgtttct acgagcggca
      781 cggcttcgtt gccgtgcaat ggaccgacgg cgacaacgaa gagggcgctc ccgacgtccg
      841 ctacgagctc cgccgcgact gaggcccaac gccgaggtgg cgacccatcc aaacgtttcg
      901 gtggtggttt gcctgggtag ccatccaaac ggacgggtag ccaggcaggt ctgcctcggg
      961 agggatgcat gtggctggct agccatgcaa accgcagccc ggatagccac agatatggtc
     1021 gaacactggt ggccaccggt gaatcgcggc gggagggaca gatggactgg acgctggaag
     1081 tggtcgtact gccggtgagc gacctcgccc gatcgatcga gttctaccgg gaccgggtgg
     1141 ggttcacgct cgatcacgag acgaagaacc agcacatgca cgttgcccag ctcaccccgc
     1201 ctggctcggg atgctcgatc gtcatcgggg atctcccttc gcagaaccag atggagccgg
     1261 gttcgatgcg ggggcttcag ctgtgtgtgg ccgatgccga aaccgcccgt ggcgagctgc
     1321 tcagccgagg cgtcgaggcc agtgagatca ccgtcttcga cgagcgcgac ggcgggacct
     1381 tcttcggttt cgccgacccc gacgggaaca cgtgggcggt ccagcagctc aaggcccggg
     1441 cgaccacgcc gctgatcccg ccagagcacc ggagccggtt cggcgcggag tagcgcaccg
     1501 tacgccacgt accgccacgt acccgccgcg taccgccgcg taccaccacg taccgccact
     1561 gccaaggctc acccgcgacc gaccgtgggt acgtcgagtg tcggtcacgg ctggcacagt
     1621 cggggcaagt agtctgtgtt tcttccccca gccgcaccgg ctgccacagg tgcaggtgag
     1681 tttggcctgc ccggaatgtc cctgcccgag ccgacctgag gagcaccgcc accgatgccg
     1741 gacagcttcg tccacctgca cgcgcacacc gagttctcga tgctcgatgg tgcagcccgg
     1801 gtcgacgagc tgctcgacga gacggtcagg ctcgggatgc cggcactggc catcaccgac
     1861 cacggcaaca tgttcggggc tttcgagttc cacaaggcag cgacgtctcg aggcgtcaag
     1921 ccgatcatcg gcatcgaggc ctacctcacc cccggcacca gcaggttcga gaagcgccgg
     1981 gtccagtggg gcaacggcgg ggaggacgac gtctccggcg gcggcgccta cacgcacctg
     2041 accatgctcg cggagacgac cgagggcatg cacaacctct tccggctgag ctcgctggcg
     2101 tcgatcgagg ggcagtacta caagccgcgg atggaccgcg agctgctcga gcggtacggc
     2161 gccggcgtca tcgcgaccac gggttgtccc ggcggagagg tccagacgct catccggctc
     2221 ggcaagtacg ccgacgccgt ccagtccgcc gccgactatc gggacatctt cgggtccggc
     2281 agctacttcg tcgagctgat ggaccacggg ctgccgatcg agcggcgggt tcgttccgag
     2341 ctcctgcaga ttgccgccga cctgagcctg ccggtcgtgg ccaccaacga cctgcactac
     2401 acacacaagg acgacgccgt cggccacgag gcgttgctct gcgtgcagtc gggttcgacg
     2461 atgaacgacg ccaaccgctt caagctcgaa ggcgacggct actacctcaa gtcgccggcg
     2521 gagatgcgcg aggtgtggcg tgaccttccc gaggcatgcg acaacacgct gctgatcgcc
     2581 gagcggtgcg acgtggcgtt cgccgaggga cgtaacctca tgccgcggtt cccggtcccc
     2641 gacggcgaga ccgaggagac gcttttcgtg aagcaggtcg aacgcggcct cgcggcgagg
     2701 tatcccgacg gcgtcacccg cgaggtccgg gcgcgggccg accgcgagat cgccatcatc
     2761 ttgcagatgg gctttccggg gtacttcctc gtcgtcgccg acttcatcaa ctgggcgaag
     2821 gcacagggga tccgggtcgg ccccgggcgt ggctcggcca ctggcgcgat cgtcgcctac
     2881 gccctcggca tcaccgagct cgacccgctc gtccacggtc tgctcttcga acggttcctc
     2941 aaccccgagc gcatcgacat gcccgacatc gacatcgact tcgacgagcg ccgccgtggc
     3001 gaggtcatcc gctactgcac ggagaagtac ggcgacgacc gggtcgcaca gatcgtcacc
     3061 tacggcacga tcaaggccaa gcaggcgatc aaggactcct cccgtgtcct cggatacccg
     3121 ttcgcgatgg gcgaacggat cacgaaggcg atgccaccgc cggtgatggg caaggacatc
     3181 ccgctgtcgg gcgtcttcga ccccgaccac agccgacatg gagaggctgg cgagttccgg
     3241 tcgctgtacg acgccgaccc cgatgtccag cgcatcgtcg acacggcccg ccagctcgag
     3301 aacctcaagc gccagtgggg cgtccacgcc gccggcgtga tcatgtcgag cgaaccgctc
     3361 atcgacgtcg tcccgatcat gcgcagggag caggacggcg ccacgatcac gcagttcgac
     3421 tacccggcct gcgagacgct cggcctgctc aagatggact tcctcgggct gcgcaacctc
     3481 accgtcatcg acgacacact cgccaacatc gttgccaacc gcgacgtcac gatcgacctc
     3541 gacaacctcg gacttgacga cgcagccacg tacgagctgc tctccagcgg tgacacgctc
     3601 ggcgtgttcc agctcgacgg ggcaccgatg cgcgcactgc tgcggcagat gaagccggac
     3661 agcttcgagg acatcgccgc ggtgctcgcg ttgtaccgcc caggcccgat gggagccaac
     3721 agccacacca actacgccct tcgcaagaac ggcaagcagg agatcaagcc gatccatccc
     3781 gagctcgagg agccgctcaa ggagatcctg gcgccgacgt acggcctcat cgtctaccag
     3841 gagcaggtca tggagatccc gcagaagctg gcgggatact ccctcggcaa ggcggacctg
     3901 ctccggcgcg ccatgggcaa gaagaagcgc gcggtcctgg aggcggagtt cgagggcttc
     3961 cgcgacggca tgctcgcgaa cggttactca cccggggcgg cgaagaccct ctgggacatc
     4021 ctggtcccgt tctccgacta cgccttcaac aagtcgcaca ccgccggcta cggtctcgtg
     4081 tcgtactgga cggcgtacct caaggcgaac tacccgtcag agttcatggc cgccctcttg
     4141 accagcgtgc gcggcgacaa ggacaagtcc gcggtctacc tcaacgagtg ccgccggatg
     4201 agcatcaagg tgctgccgcc cgacgtcaac gagtccgagg ccgacttcac cccggtgggc
     4261 aaggacgtcc ggttcggcct gtcggcgatc cgcaacgtcg gcaacaacgt cgtcagcggt
     4321 ctgctcggcg cccgcgagag cgagggcaag ttcaccgacc tcaacgactt cctcgacaag
     4381 gtcccggcgg tggtgtgcaa caagcgcatc ctcgagtcgc tgtgcaaggc cggggcgttc
     4441 gactcgttgg gctacacacg ccgtggtctg gtcgccatcc acgaggaggc ggccgactac
     4501 tacgtcgacc tgaagaagaa cgaggagttc ggacaggact cgctgttcgc cggtctcgac
     4561 gacgtgggtt tctccgcggg gagcgtggag gtgcccgacc tggcggagtg ggacaagaag
     4621 acgctgctcg ccttcgaacg cgacatgctc ggcctgtatg tctctgacca tccgctcaac
     4681 gggctcgagc acgtcctcgc ctcgacaagc gatgtcacga tcggtcagct gatcaccgac
     4741 gacagccgcg aagagggcgc tgtggtgacg gttgccggcc tcatcacgtc ggtcgtgcgc
     4801 aagaccacga agaagggtga cctgtacgcc gtggtcactg tcgaagacct cgagggcgcc
     4861 atcaacgtca tggtcttccc acgtgactac cagctttcgt cgacgttgct cgtcgaggac
     4921 gcgatcgtgc tcgtcaaggg caaggtcaag tacagccgtg acgaagcact cgagctgacc
     4981 gcgctcgagg tcagcgtgcc cgacctgacc aaggggatca ccaatggccc ggtcgtgatc
     5041 tcgatgcccg cagtgcgcgt gaccccgccg atcgtcgacc agctcaagga cgtgctggcc
     5101 acgcatcccg gcgttgccga agtgcacctt cgtctgcagt cgcagggacg aacgatgctg
     5161 atgcggctcg acgagcggtt gcgtgtcacg ccgtcacccg ccctgatggc tgacctgaag
     5221 gcgctgctcg gcccctcctg cttgtcgagc tgaccttcgt gtccgcttat cgagcctgtc
     5281 gagatcgagg gatcttgtgg gtctcgacaa gctcgaccgc cggttccgta cgccgcctag
     5341 actccaccac gtgagcagcg ctggcccgcc gtcatccacg acgatgtctt cctcaccgga
     5401 gccgatgccc cctgtcgcat catcgccgca gggcatgttc gagacgttcg aagatccacc
     5461 gagctcgagg ctggagcggc tccgcttcgt gtcggtgtgt ctggtggtcg gcgtactcgc
     5521 cgccgtgccg gctgcatggc tgtgggtcga gctggccgac ccgccgtcga cccgtctctc
     5581 cagcgacggc ctggtcttcg gagaactggc gttcaacgac gtgacctcga tcacgctctg
     5641 gttcgtggtg gtcgggttcg cctggggggt cgtgctcggc ttggtggcca cctcgctcgg
     5701 acgacggcac ggactggtga ccgtggcggc agtcctgctc atgagcgtgg tgggctcgac
     5761 agtgatgctc tggtgcggga tccacctgtt cggtccggat cacccggtgg acttcgtcgc
     5821 gctcttcaac gccgagcccc aggagcggcg gcagatgctc gacggttttc attcggggga
     5881 cctgctcgtc agcagcctgc ggctcacgac ctgggtggcg ctgctggcct ggccgatcgg
     5941 agccatggcc ggggccctcg gcggcacgta cctgtggcca aaggcgccga agccgccgca
     6001 gatgcccccc tcgtgaagta ccctcgcctt tccgtgacgg tcacgtcgga cgagtcgaca
     6061 ggcaagggga cctgatgtcc agcaacactc cgcctcccgg cagcggggga ccgccctggg
     6121 gccagccgca gcaacccggc cagggctggg gtcaacagcc tcccggcggg cagcagccag
     6181 gcgggtacgg ccaaccgggt ccctccgggc aaccgcccgc gccaggacag ccaggtccct
     6241 acggacagcc cggccagtac gggcagcccg gccagcccgg cccctatggc cagcccggcc
     6301 agcccggacc gttcgagcag gcgtccggac ctggggggcc cggcaccggg gagccccaag
     6361 gcggctcggg tggcgctcgc cgcggactga ccgcgctgat cgtcgcggtc gtggtcgtcg
     6421 ttctggcagc gggcatcgcc gtcgccattc cgttcatcaa gaacaagctc agctctgccg
     6481 gtccgcaggc cgccgaggcg atccccgccg acgccgtctt ctacgtcggc gtcgacctcg
     6541 acccgtcggc ggagcagaag atcaacgcgc tgcggttcct caaccacttc ccggccttca
     6601 aggaagagac cggtgtcaca gacgcgaaca ccgacgtacg caagacgatc ttcgacaagc
     6661 tgctcgagtc cgaaccgtgc ccgggagtga cgtacgacga cgacatcaag tcctggatcg
     6721 gcagcaagtt cgcgttcgcc gggatgccgc cggcgtcagg gagcagcgaa cccgtggtgc
     6781 tcggagcggt ggaggtcacc gatcagtcgg aggccaagac cgggatccgc aagctggccg
     6841 agtgtggatc cgacccgtcg acgttcggtc tggccttcac cggcgactac gccgtcatcg
     6901 cagaaacgca ggatgaggcc gacaagtacg ccgacgcggc gtcgagtgcg tcgctggccg
     6961 acgacggaga cttctccgcc gacatggacg agctcggcga cctcggggtg gccaccagct
     7021 gggtggacat caagggtgcg atcgacctgt tcggctccga catctccgcc gaggacgatg
     7081 aggcggggct cgacttcatc cgctccgcct accagcgcgc ggccatgacg ttccgcttcg
     7141 agagcgacca cgccgagttc gcctcggcgg tgtacggcga cctgcctgac gtcgaccacg
     7201 gcaccaacca gatcgtcaac ctcccggact cgaccgtgtt ggcgatgtcg gaggcagggg
     7261 gcggcaaccg catcgcggca cagtgggacg acctgatgga ggtcgcgaag tcgtccgggg
     7321 tcgacgtcga ccaggagatc cagaagttcg aggaccggac cggcctggtg ctccccgacg
     7381 acctcgagac cgtgctgggg gacaacctcc tgctggccgt cgacggtgac ggcctgacgg
     7441 ccgacgccct caactccggc gatgtggcgc agatcaacgc cggcgtgcgg ttcagcggcg
     7501 acaaggccga actggaccgg ctctaccaga aggttctcga tctgatcgag gaggagaccg
     7561 gaacgcaggc cgagacggtc aagaaggaca ctgacgacgg cttcgtgctc gcgacgaacg
     7621 acagctatgc ggacaagctg gccagtggcg gcgacctcgg cgactccgac gcgttcacgt
     7681 ccgtcgtcga caacggcgcc gaccaggagt tcgtcctctt cttcaacttc gaccaggtgg
     7741 agaaccagat cctgcaggcc gcgcaggacc aaggcgcgtc agcggaggct ctcgacaacc
     7801 tccgtccgct ccaagcggtg gcgatcacca gcgacaccga cgggaagtac gcccacggca
     7861 ccctcgtcat ctccgtcaac gactgacccc gctgggcgag ctggtcgagt ggtccgctga
     7921 tcgagtggcc cgctgatcga gtggcccgct gatcgagcct gtcgagatca ccgggcgctg
     7981 gagcttcggg gtctcgacaa gctcgaccgc cgaagcacgc tgatcgccgg gtccgctgat
     8041 cgagcctgtc gagatcaccg ggtgctggag tcttcggggt ctcgacaagc tcgaccgccg
     8101 aagggcgctg accgccgggt gctggagtct tcggggtctc gacaagctcg accgccgaag
     8161 ggcgcccggt tgcggttggg gccttggcgt cttgcgcctg atcccgaggt ggcggatttc
     8221 gtccaggaca cgcgacgggt gggcgaaaac cgccacctcg gctgaggggt cggcgcgaac
     8281 cgatactggc cgtttggctg gggctgaact ggttagttgg ggctccaggc ggtgcgggcc
     8341 atcgaggtgg cggggagtga ggggattgcc gccaccgcct gcaattcggc acgcatgatc
     8401 gacgtaccga gacgcagccg actcacgaca tccggggcct ccaacatggc ctgccgctcc
     8461 gcgaggttga gcacgagcgc cgcggcgagc gtgtacgaca ggtcgaccgg gctcgtgggc
     8521 gccggacctt cgaggatgtc gtctccccgc agccgggcga ccttctcacg gtagacggcg
     8581 aacagcgagg cggcctgctc cgccgcctcc ctggcatcgg ccggcgacgt gcctccggac
     8641 gcatcgggca gccactcgac gtccgcacgg aggtagtcct gcgacgggtc gaggctctcg
     8701 acgtggaacc ggcggcggcc gatcaccatg atctcgaacg tgccgtccgg gttcgccgtg
     8761 acctccgtga cgagcgctgc gcagccggtc cgctggatcg tgtggacccc acgtctgccg
     8821 acctcgtagc cgaccttgat ggcgacgacg ccgaactcgc gagcagcgtc gggaggcagc
     8881 gcgatgaggt ccttgacgag cattcgatag cggtcctcga agatgtggag aggcatgacc
     8941 accccgggaa acagcaccgt gttcaacggg aagagaggca atggctcgcc cacgtgtcga
     9001 acgtagcacc gtgacggctc gctcccgagg cttggtgaga ctgtcgcggc ctgtgtcgag
     9061 gcaagatctc tagacttgtc gcatgattcg ccgcatcgac gtacgcggga gggcggcgac
     9121 cggtgaagcg tccgactacc ggtcgctggt gccacgtgcg gcgttcgacg tcgaggcagc
     9181 catggagcgg gtgcgcccgc tctgcgaggc cgtacgccgc gacggccacg cggaactggt
     9241 ccgccaggcc gagcggttcg acggcgtccg gatcggctcg gtccgcgttc ctggcggggc
     9301 actgcacgat gcgctggatg ggctcgaccc gtcgctgcgg gccagtctcg aggagtccgt
     9361 acgccgcctg cggacgacgt gcgaggcgga gctgccggcc gacgtgacga ccgtcgtcac
     9421 aggtggtggc acggtgacgc agcgtaacgt cccggtccgg cgagtcggcc tctacgtgcc
     9481 cgccgcgaag gcgccgctga tctcgtccgt ggtcatgaac gtcgtaccgg cccaggtcgc
     9541 gggcgtgtgg tcgctcgcac tggcgagccc ggcgcagcgc gaccacgacg ggctgccgca
     9601 cccgtcgatc cttgccgcgt gcgccgtgct gggcgtcgac gaggtgtacg ccgccggggg
     9661 agcgggggcg atcgcgatgt tcgcctacgg ataccagccc gaccagagca ccgccaggtc
     9721 cgacccggga tgtgtcccgg tcgacctggt cacgggaccc gccaacatct acggcgtcgc
     9781 agcgaagcgg ctccttcgcg gcgtcgtggg gatcgacgcc gaggccggac cgaccgagat
     9841 cgccatcctg gcggacgagt ccgccgaccc gacgttcgtg gcggccgacc tgatcagcca
     9901 ggcggagcac gacccgatgg ccgcgagtgt gctcgtcacg ccgtcgctgc gactcgtcga
     9961 ggcggttgag gacgagctcg cccgccaggt cgctgcgacc aagcacacgg agagggttca
    10021 cgaagccttg agcggccagc agtcggggat cgtcatcgtc gaggacctgg agcagggtga
    10081 ggacgtcgtc aacgcctacg cagccgagca cctggagatc cacaccgtcg acgcgcccgc
    10141 ccgcgccggc cggatcatga acgccggcgc gatcttcgtc ggaccgtacg cgcccgtgtc
    10201 gctcggtgac tatgccgcag gttccaacca tgtgctgccg acggcggcct gcgcctgtca
    10261 ctcgtccggc ctgtcggtgc ggagcttcct caagaccgtt caggtcgtcg actacaccgc
    10321 gcaagcgctc gccgacgtcg cgggaccggt gacgacgttg gccgaggccg aggacctgcc
    10381 ggcacatggc gaggcggtcc gcatccgctt ggagcgctga tgaccggcga tgatctgccg
    10441 gtccgcccgg agctggccgg gatcgagccg tacggcgccc cgcagctcga ggtttcagtc
    10501 cggctcaacg tcaacgagaa cccgtacccg ccgccacccc acgtggtcga ggcgatggcc
    10561 gcggcggtcc gcgacgcagc caccacactc aaccgctatc ccgaccggga ggcccgcgca
    10621 ctcaggacgg cactcgctga ctacctgggg catgggctga ctgcggaaca ggtctgggtg
    10681 gggaacggat cgaacgaggt catgctgcac gtcctccagg cgttcggagg agccggccgg
    10741 accgtgttgt cgttcgcccc gacgtactcc atgtaccccg agtacgcacg cgacacgtcc
    10801 acccgctggg tcgtcgggcg gcgtaccgac gacttcggca tcgacatcga ccacgccgtc
    10861 aacctgatcg ggcaggcgca gccgcacgtc gtgctcctcg cgtcgcccaa caacccgacc
    10921 ggcaccgcgc tgccgctcga cgccgtcaag gccatctgca ccgtcgcgcg aggcgtggtc
    10981 gtcgtggacg aggcgtacgc cgagttccgg cgctcaggta cgccgtcggc gctcagcctg
    11041 ctcgacgact tcccgaggct ggcggtgaca aggacgatgt ccaaggcgtt cgcgctcgcc
    11101 ggaggccgtg tcggctacct ggcggccagt cgcgacttcg tcgacgcgct gcgcgtcgta
    11161 cgtctcccct accatctgtc cgcgaccacc caggcggttg cccgggtcgc gctcgagcac
    11221 cgcgcggagc tgctcgcgcg ggtggcggac cttcgcgccg aacgcgacgc caccgctgca
    11281 tggctgcggc gccagggtct cgacgtcgcc gactccgacg ccaacttcgt cctgttcggt
    11341 acgttcggag atcgccacgc cgtctggaag gatctgctcg accacggtgt gctcattcgc
    11401 gagaccggcc ccgaggggtg gctgcgtgtc tccatcggca cggctgccga gatggacgcg
    11461 ttccggcagg cgttgcaggc caccctgtcc agcctcgagg aaggatcgtc atgacgactc
    11521 gtgcggcgcg gatcgaccgg gagaccaacg agtccaaggt cgtggtcgag ctcgacctgg
    11581 acggctcagg ccgtgccgag gtgtcgaccg gcgtgggctt ctacgatcac atgctcacct
    11641 cgctcgctcg gcatgcactg tgggacctga cggtccaagc acaaggtgac ctgcacgtcg
    11701 acgcccatca caccgtcgag gacgtggcga tcgtgctcgg tgaggcgatg cgcacggcac
    11761 tgggggacaa ggccgggatc cggcgcttcg gtgacgccct cgtcccgctg gacgaggcgc
    11821 tggtgcagtg tgctgtcgac gtgtcgggcc gcccctactg cgtgcagaca ggggagccgg
    11881 ccggtcaggc gtatgcagtg atcggcgacg ggtacgccgg ctcgttgact cggcacgtgt
    11941 tcgagtcgat gagcgcacac gcgtcgatcg ccatccatgt ccgggtgctg gccggccgcg
    12001 acccgcacca cattgtcgag gcgcagttca aagcactcgc tcgggccctt cgtgacgccg
    12061 ttgcgcacga cccccgcgaa ccgggagtac ccagcacgaa gggacacctg tgacggctgg
    12121 tgcgcgtccc cgggttgtcc tgctcgacta cggctcgggc aacctgcggt ccgctgagcg
    12181 tgcgctcacg cgggccgggg ccgacgtcac catcgacgct gaccacgcga cggcactcgg
    12241 tgccgacggt ctggtcgtac ccggcgtggg ggcgtttgcc gcctgtatgg ccgggctgac
    12301 cgccgtacgc ggccccgaga tcatcggacg acgactggct ggagggcgcc cggtcctggg
    12361 tatctgcgtc ggccaccagg tgttgttcga gcagggcatc gagcacggcc aacccaccgc
    12421 tgggtgcggg gaatggcctg gtcgggtcga gcgcctgcat gccgcggtgc tgccgcacat
    12481 cggttggaac acggtcgagg caccacccga gagcgcgatg ttcgccggcg tggagcagga
    12541 gcgcttctac ttcgtgcact cgtacgccgc ccgggcctgg acactcgtca ccaacgggcg
    12601 aactcgcgcc ccactcgtca cctggacgac ccacgagacc gacaggtttg tctccgcggt
    12661 ggagaacgga ccgctgtggg cgacgcagtt ccatccggaa aagtccggcg acgcgggagc
    12721 cgttctgctg cgtaactggg tccaacagtt atgaaccgac gtatcgcggc ggccctgacc
    12781 ctcgccctcc tcacgttcac cactgcatgc ggttccagcg agtccggcgg cgagtcatcc
    12841 ggcggggacc cgacgtcgac gtcctcggat gcgacgaacg acgccgaccc atccaccgac
    12901 gccgtgagtg tcatgttcca caagagtggc ggtctgcggc cggtcgacga acgaacgttc
    12961 tactccgctg acgcgccgcc gcctgccggg aagacccggg ctgacgtcga cgacgtcctc
    13021 gacgcggcgt cgaaccccga tctgcgcgat gtcgacgccg ctccgatgcc agaaaacgtg
    13081 tgttgcgacc gccaggagta catggtcacg atcagctacg ccgacggcac gtcggtgacc
    13141 taccgcacga tcgacggtct tcagcagccg gtcgtgctgg agcgcttcct cggcatgctg
    13201 tgatgggttc gtgacgacac tggagctgct gcccgccgtc gacgtgtccg gtggccgtgc
    13261 cgtccagctc gttcagggcg tggccggcag cgagcgcgtc ttcggtgacc ccgtcgccgc
    13321 tgcgctgcgc tggcagcagg cgggcgcgca gtggatccat ctcgtcgacc tggacgccgc
    13381 gttcggtcgc ggtgacaaca ggtcgctgct gcgggaagtc gtggccgcgc tcgagatcga
    13441 cgtcgagctc tccggcggta tccgcgacga cgagagcctg gccgctgcac tgtccaccgg
    13501 ctgccgccgg gtgaacatcg gcaccgcggc actggaggac ccggcatggt gtgcgcgggt
    13561 gatcgctgcg cacggcgacc gtgtcgcgat cgggctggac gtgcggggca caacgctcgc
    13621 ggcgcgtggg tggacgcgcg acggtggcca cctccacgag gtgctggcgc ggctggacca
    13681 ggccggctgc gccaggtacg tcgtcaccga cgtcaagaag gacggcatga tgcagggccc
    13741 gaacctcgat ctgctccgcg acgtctgcgc gcggacgcag gcacccgtgg tggcgtccgg
    13801 tggtgtctcc acgatcgacg acctggtggc catctccggc ctcgtggacg acggcgtcga
    13861 aggggcgatc atcgggaccg cgttgtacac cggtgcgttc acgttggagg acgcactggc
    13921 cgcggtggac aagggatgac cgttgccgtg cgcgtcatcg cgtgcctcga cgtcgacgac
    13981 ggccgggtcg tcaaaggcgt gcgattcgcc gacatccgcg acgtcggcga tcctgtcgag
    14041 ctggccagtc ggtacgacga gcagggcgcc gacgagctca ccttcctcga catcaccgcc
    14101 tcccagcagc gccgcgacac ggtcctggac gtcgtccggc gtaccgcgga agaggtgttc
    14161 atcccgttga ccgtgggagg cggagtccgc accgtcgacg acgtcgacgt actcctccgt
    14221 gccggtgcgg acaaggtggc gatcaacacc ggggcgatcg aacgacccga ggccatctcg
    14281 gagatcgccc agcggttcgg ccgtcaggtg ctggtgctga gcgtcgacgc ccggcgcgac
    14341 tcgcagacac gatcagggtt cgaggtgacc acgcacggcg gcagccaagg cagcggcctc
    14401 gacgcggtcg agtgggctcg cgcgggggcg gagcacggtg ccggcgagat ccttctcaac
    14461 gcgatggacg ccgacgggac acagcaaggg ttcgacctcg agctgatcgg cgcgatccgt
    14521 gcggtcgtca cgatcccggt catcgcgagt ggcggggccg gagtggcgga gcacttcccg
    14581 ccggcgatcg aggccggggc cgatgccgta ctcgcggcca gcgtcttcca cgaccgactg
    14641 ctcaccgtgg ccgacgtcaa gcgtgccttg gccgccaccg gccacccggt ccgctgagag
    14701 ctcagaagcc gggccagtgc cgcgtcaacc aacgttgctc gtcgtgagcg gtgtccgggt
    14761 ggatgagctg caaggcagag gagggaggcg atgcgcagca tcgttgaccg acgataacgc
    14821 cgcagatcgc cgcccgggcg gcgcgcaaca gggcga
//
LOCUS       DATBFZ010000318         1951 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_3169803, whole genome shotgun sequence.
ACCESSION   DATBFZ010000318 DATBFZ010000000
VERSION     DATBFZ010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 1951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1951
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3169803"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            158..964
                     /locus_tag="VH419_09875"
     CDS             158..964
                     /locus_tag="VH419_09875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043962.1"
                     /translation="MDRAVLVRTIPRQRMLAILAACGVVAMALMVSPAAAGAAAPGIL
                     PPQASVGGRTYSQWSAAWWQWQLGSQNVPTNPSADPKPGTSAQPEAVDCHLGQTGQVW
                     FLAGISFAQSFTTTYRSCSVPAGVFLFFPVFDAWNDNLNCPGLPPLPNTARQLAQNVR
                     QQVDAIDPGTMSVTIDGANVPGLRDSSTAFRAAARGFSYTLPDNNLLSPFCATPFPAG
                     TTPPRPGAFADGVYVLRAPLPVGVHQLHWTAQAGKGAIVQDVNYTITVRG"
     gene            1121..>1951
                     /locus_tag="VH419_09880"
     CDS             1121..>1951
                     /locus_tag="VH419_09880"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF012470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HEY7043963.1"
                     /translation="MGAQRKILVGSALAVAGLVAATTAGAGSSDGDRTREVRQAIDGG
                     KAKNVIMFLGDGMGDSEITIARNYQVGAAGRLSMDTLPLSGEYTTYAVQKGTTDLPDY
                     VTDSAASGTGWSTGHKTYNNAVSVDPVTGAPLTTILEQAQAAGFKTGNVGTAELTDAT
                     PAVLDSHISLRGCQGPTDMATCPNETKQAGGLGSIAEQTVDHNVEVVLGGGKQRFDQT
                     VTGGPFAGQTVTQQAQSQGYNVVTTRAGLDAAQAGTKVLGRFAPGNMDLEWTGPVAAP
                     Y"
BASE COUNT          333 a          701 c          624 g          293 t
ORIGIN      
        1 gaaacgttat cggtaaccac atcatctctc agacccgcga ttgaggatcc acatcccggc
       61 ttcggcgtga gacacgtcac tattgacgac cgaaggccac aggcataccg tcacgatcac
      121 gccgtccgac ctaacggtcg gggcgaaggg agggcgtatg gatcgcgccg tgctcgtccg
      181 gactattccg cgccaacgca tgcttgcgat cctggccgcc tgcggcgtcg tggcgatggc
      241 gctcatggtg tcgccggccg cggccggcgc cgccgcaccg gggatcctcc cgcctcaggc
      301 gtcggtcggc ggccgcacgt acagccagtg gtcggccgcc tggtggcaat ggcaacttgg
      361 atcccagaac gtgccgacga atccgagcgc cgatcccaaa ccaggcacgt cggcgcagcc
      421 ggaggcggtc gactgccatc tcggccaaac cggccaggtc tggttcctcg ccggcatctc
      481 gttcgcgcaa tcgttcacga ctacctaccg cagctgcagc gtccccgcgg gcgtgttcct
      541 gttctttccc gtgttcgatg cctggaacga caatctcaac tgtccggggc tgcccccgtt
      601 gccgaacacc gctcggcagc tcgcccagaa cgtccggcag caagtcgatg ccatcgatcc
      661 cggcacgatg agtgtgacca tcgacggagc caacgtcccg ggactccgtg acagctcgac
      721 tgcgttccgc gccgcagcac gagggttctc ctacacgctc ccggacaaca atctcttgtc
      781 ccccttctgc gcaacgccgt tcccggctgg cacgacgccg ccgcggcccg gcgcctttgc
      841 cgacggcgtc tacgtacttc gtgctccact accggtggga gtgcatcagc tccactggac
      901 ggcccaggcg gggaagggcg ccatcgtcca ggacgtgaac tacaccatca ccgtacgcgg
      961 ctgatcccgt tggaaccttg gtgtcacctg tggattggcc gtgggttgtc ctccaactcg
     1021 ttgtgagcct gccacctccc tgacacccac gcgccagtgg ccgttcactc ccgtctgatg
     1081 gcgtgcatcc gggcgacgac gaaagggaga gggaaacgcc atgggtgcac aacgcaagat
     1141 cctcgtcggc agcgcgttgg cggtggccgg cctggtcgcg gccacgaccg ctggcgcggg
     1201 gagcagcgac ggcgaccgga cacgagaggt ccgccaggcc atcgacggcg gcaaggccaa
     1261 gaacgtgatc atgttcctcg gtgacggcat gggcgactcc gagatcacga tcgcccgcaa
     1321 ctaccaggtc ggtgcggccg gtcggctgtc gatggacacg ctgccgctgt cgggcgagta
     1381 caccacctac gcggtgcaga agggcaccac cgacctacct gactacgtca cggactcggc
     1441 cgcgtcgggg accggttggt cgacggggca caagacgtac aacaacgccg tctcggtcga
     1501 ccccgtcacc ggcgctcccc tcacgacgat cctcgagcag gcccaggcgg ccgggttcaa
     1561 gaccggcaac gtgggcaccg ccgagctcac cgacgccacg ccggcggtgc tcgactccca
     1621 catcagcctg cgcgggtgcc aggggccgac ggacatggcc acctgcccca acgagaccaa
     1681 acaggccggc gggctgggct ccatcgccga gcagaccgtc gatcacaacg tcgaggtggt
     1741 gctgggcggg ggcaagcaac gcttcgacca gaccgtcacc ggcgggccgt tcgccggcca
     1801 gaccgtgacc cagcaggccc agtcccaggg ctacaacgtg gtgaccaccc gggcgggcct
     1861 cgacgccgcc caggccggca ccaaggtcct cgggcggttc gcgccgggca acatggacct
     1921 cgagtggacc ggtcccgtgg ccgctcccta c
//
LOCUS       DATBFZ010000319         5371 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_4705239, whole genome shotgun sequence.
ACCESSION   DATBFZ010000319 DATBFZ010000000
VERSION     DATBFZ010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 5371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5371
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4705239"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1608)
                     /locus_tag="VH419_09885"
     CDS             complement(<1..1608)
                     /locus_tag="VH419_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020577281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HEY7043964.1"
                     /translation="MHSKLEDAKNQLIADAAKLAEQRKGGVVPDVEGTHHLLSLFYRH
                     VAPEDLLDRSAVDVYGAALSHYRLAAHRPQGTANVRVFTPTVDEHEWDADGHTVVEVV
                     TDDMPFLVDSVTMALTAEDRAIHVVIHPQVVVRRDVTGALSEVCDADQVTADEADVMR
                     ESWMHIEIDRETDPAALQEIEAHLAKVLRDVREVVEDWKKMHEQALTLVTELEESPPP
                     LDDTEVSEGKALLSWLADDHFTFLGYREYQLQTIEGADALRAVPGTGLGILRSDQDMS
                     ASYGKLPPAVAAKAREKQLMIVTKANSRATVHRPVYLDYVSVKKFDEGGEVVGERRFL
                     GLFSSAAYTESLTRIPVLRDKARQVIAGAGFSPLSHSGKALMDILETYPRDELFQTSV
                     EDLLPVAEAVLHLRERRQLRFFVRRDVYGRYLSCLVYLPRDRYTTQVREKMQSILKET
                     MGADTIEYTARVSESVLARLHFVVRAAPGKTLHDFEEDELEAKLAEATRSWTDDLLVA
                     LHEQFGEEDGSRLAKSYGKAFPEAYKED"
     gene            complement(1763..2626)
                     /locus_tag="VH419_09890"
     CDS             complement(1763..2626)
                     /locus_tag="VH419_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012919326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HEY7043965.1"
                     /translation="MTTEQAPQAESGTDLPDEDAVVGDWQGEDIHFGEQGAKLTYGSY
                     LQLGTLLDAQRLQSEPPAHDELLFITIHQVYELWFQQLLHEAETARDAMTDGRLWWAK
                     HLLSRMHVIERVLVDQIAVLETMTPQDFLVFRQELAPASGFQSVQYRELEFVSGAKDP
                     SFVRRFRGLTDAERGRLERRLVEPTLWDAFVHALSSRGLPTTGDEDIRSSLRTVAHDR
                     EQYADVWELAEALIEHDELAAAWRARHVTMVERMIGTKTGTGGSSGGAYLRSRLPLRY
                     YPLLWDLRSEL"
     gene            2667..3158
                     /locus_tag="VH419_09895"
     CDS             2667..3158
                     /locus_tag="VH419_09895"
                     /inference="COORDINATES: protein motif:HMM:NF022161.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2505 domain-containing protein"
                     /protein_id="HEY7043966.1"
                     /translation="MKISAEISYPGATPEQAYGLSRDPEFRAAVCEATHALDYDVRVD
                     EHDDETASVVVTRTMPAEVPDFLKKMVGETIGVVQTEEWGKPDGDGQRTADVVVSISG
                     QPAKMTGTAAIVRTDEGAAMRIEGDLKVAIPIFGKKVEPEIAKGIYAAIKQEQRTGTS
                     WLS"
     gene            3269..3544
                     /locus_tag="VH419_09900"
     CDS             3269..3544
                     /locus_tag="VH419_09900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043967.1"
                     /translation="MTYLNHPGYSDNPRDEAPGSPAELLMLSGVVRECPDCAGEQIFV
                     AADDVVEPGHEAGTGAYCCTACGAAILLDPLLERQRMQSGAAEHDVA"
     gene            3586..4344
                     /locus_tag="VH419_09905"
     CDS             3586..4344
                     /locus_tag="VH419_09905"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY7043968.1"
                     /translation="MSFDVSADAYARFMGGFSEPLAPQFADRAEVRAGHRVLDVGCGP
                     GALTAVLVDRLGNDHVTAVDPSASFVEATRRRLPGVEIRSAVAEDLPFDDDTFDRAVA
                     QLVVHFMTDPVAGIAEMARVTRPGGVVAACVWDHGGGTGPLSPFWRVVHDLDPDAFDE
                     TQLAGAREGHLVELFTTAGLDAPAQSRLTVERHYGDFDEWWEPFTYGVGPAGGYVDGL
                     DVDARERLRQHAEDELPSGAFNLTASAWCATAVV"
     gene            complement(4361..>5371)
                     /locus_tag="VH419_09910"
     CDS             complement(4361..>5371)
                     /locus_tag="VH419_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009075672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY7043969.1"
                     /translation="GNTVIWKPSPTQQFAAHFVMRLLEAAGIPPGVINMLPGDGVAVS
                     EAALTHPDLAGIHFTGSTATFQHLWRTVGANISSYRSYPRIVGETGGKDFVVAHPSAD
                     HDVLRTALIRGAFEYQGQKCSAASRAYVARSVWRKVRKDFLAEVDSLTMGDVTDFSNF
                     MGAVIDARAFDKHKKAIARVKRSSRTQVLTGGTTDGAVGYFVRPTVLESSDPTDAMFK
                     TEYFGPILSVHVYDDADYDKAVDQMESFAPYALTGAVIAQDRRVIVAAMEKLRFAAGN
                     FYVNDKPTGAVVGQQPFGGARASGTNDKAGAAQNLMRWTSPRSIKETFAPPTSYRYPH
                     MG"
BASE COUNT          835 a         1825 c         1832 g          879 t
ORIGIN      
        1 gtcctccttg taggcctctg ggaacgcctt gccgtacgac ttggcgagcc gggacccgtc
       61 ctcctcgccg aactgctcgt gcagcgccac cagcaggtcg tcggtccagg accgggtcgc
      121 ctcggcgagc ttcgcttcga gctcgtcctc ctcgaagtcg tgcagcgtct tccccggcgc
      181 ggcgcgaacg acgaagtgca ggcgcgcgag cactgactcg ctgacccggg cggtgtattc
      241 gatggtgtcg gcgcccatcg tctccttgag gatcgactgc atcttctcgc gtacctgcgt
      301 cgtgtagcgg tcccgcggca ggtagaccag acacgacagg tagcggccgt agacgtcgcg
      361 gcgcacgaag aagcgcagct gccggcgctc gcgcaggtgc agcacggcct cggccaccgg
      421 aagcaggtcc tcgaccgagg tctggaagag ctcgtcccgc gggtaggtct cgaggatgtc
      481 catcagcgcc ttgccgctgt ggctcagcgg cgagaagccc gcccccgcga tgacctggcg
      541 ggccttgtcc cggagcaccg gaatgcgggt caggctctcg gtgtacgccg cggagctgaa
      601 cagcccgagg aaccggcgct cgccgacgac ctcgccgccc tcgtcgaact tcttgacgct
      661 gacgtagtcg aggtagacag gacggtgcac ggtggcgcgc gagttggcct tggtgacgat
      721 catcagctgc ttctcgcggg ccttcgcggc cacggccggg ggcagcttgc cgtatgacgc
      781 cgacatgtcc tggtcggagc ggaggatgcc gagaccggtc cccggtacgg cgcgcagggc
      841 gtcggcccct tcgatcgtct gcaattggta ctcgcggtag ccgaggaacg tgaagtggtc
      901 gtcggccagc cagctcagca gggccttgcc ctcggagacc tcggtgtcgt cgagcggcgg
      961 cgggctctcc tccagctcgg tcacgagcgt caacgcctgc tcgtgcatct tcttccagtc
     1021 ctcgacgacc tcgcggacgt cgcgcaagac cttggcgagg tgcgcctcga tctcctggag
     1081 cgcggccgga tccgtctccc ggtcgatctc gatgtgcatc cacgactcgc gcatcacgtc
     1141 cgcctcgtcg gcggtgacct ggtcggcgtc gcagacctcg ctcagcgcac cggtgacgtc
     1201 ccggcggacg acgacctgcg ggtggatgac gacgtggatc gcgcggtcct cggccgtcag
     1261 cgccatcgtc accgagtcga cgaggaacgg catgtcatcg gtgacgacct cgaccaccgt
     1321 gtgaccgtcg gcatcccact cgtgctcgtc gacggtgggc gtgaagacgc ggacgttcgc
     1381 cgtgccctgt ggccggtgcg ccgcgagccg gtagtggctc agcgccgcgc cgtagacgtc
     1441 gaccgcactg cggtcgagca gatcctcggg cgcgacgtgt cgatagaaga gactcagcag
     1501 gtggtgtgtt ccctccacat cgggaacaac cccgcccttg cgctgctctg ccagcttcgc
     1561 cgcgtccgcg atgagctggt tcttggcgtc ttccagcttg ctgtgcatga gaccaagacc
     1621 tacttctggc cagccgcgcg tggccgtcct accggccacg gtcgcacgcc gttgtgcgac
     1681 agtatcgaca ctaaacccgt aggcgccgcc gcgtatgcag ggtgacaatg acggttcgtc
     1741 acccgctgcg agccggatgc gatcaaagct ccgagcgcag gtcccagagc agggggtagt
     1801 agcgaagcgg caacctgctc cgcaggtacg cgccacccga cgagccgccc gtccccgtct
     1861 tggtcccgat catccgctcg accatcgtca cgtggcgggc gcgccaggcc gcggcgagct
     1921 cgtcatgctc gatcagcgcc tcggcgagct cccacacgtc ggcgtactgc tcccggtcgt
     1981 gcgccacggt ccgcagcgag gagcggatgt cctcgtcgcc ggtggtcggc aggccacgag
     2041 acgagagggc gtggacgaat gcgtcccaca gggtgggctc gaccagcctg cgctcgagcc
     2101 ggccacgctc ggcgtcggtc agcccgcgga agcggcgtac gaacgacggg tccttggcgc
     2161 ccgagacgaa ctcgagctcc cggtactgca cggactggaa gccgctggcg ggggcaagct
     2221 cctgccggaa caccaggaag tcctgcggtg tcatggtctc cagtacggcg atctggtcca
     2281 cgaggacgcg ctcgatcacg tgcatccgac tcagcaggtg tttcgcccac cacagccgac
     2341 cgtcggtcat cgcgtcccgg gccgtctccg cctcgtgcaa cagctgctgg aaccagagct
     2401 cgtacacctg gtggatcgtg atgaacaaca gctcgtcgtg cgccggcggt tccgactgga
     2461 gccgttgagc gtcgagcagg gtaccgagct ggaggtagct gccgtacgtg agcttggcgc
     2521 cctgctcgcc gaaatggatg tcctcgccct gccagtcgcc gaccaccgca tcctcgtccg
     2581 gcagatcggt gccgctctcc gcctgcggag cctgttcggt cgtcaccggg ccaggttatc
     2641 ggtggcgtcg atggcatgat gcggccatga agatcagcgc tgagatctcc tacccgggcg
     2701 ccactcccga gcaggcctac ggtctgagcc gagatccgga attcagagcc gcggtctgcg
     2761 aggcgacgca cgcgctcgac tacgacgtgc gggtcgacga gcacgacgac gagacagcct
     2821 cggtcgtcgt gacccgcacc atgcccgccg aggtgccgga cttcctcaag aagatggtcg
     2881 gcgagacgat cggcgtcgta cagaccgaag agtggggcaa accggacgga gacgggcagc
     2941 gcacggccga cgtcgtcgtc tcgatcagcg ggcaaccggc gaagatgacc gggactgcag
     3001 cgatcgtgcg gaccgacgag ggggctgcga tgcgaatcga gggggacctg aaggtcgcca
     3061 ttccgatctt cggaaagaag gtcgagcccg agatcgccaa gggcatctac gctgcgatca
     3121 agcaggagca gcggacgggc acgtcctggc tgtcctagcg tcaccatcag cctgctcgcc
     3181 gtcgcagcag tcccaccagc ggctggaatc ccggggcatg ggcgcgtcgg tcgacgagac
     3241 ctccggccga ccgtgccact gtggcgccat gacgtacctg aaccatccgg ggtactccga
     3301 caacccacgt gacgaggcac ccgggtcacc agccgagctg ctcatgctga gcggggtcgt
     3361 gcgcgagtgc cctgactgcg ccggtgagca gatcttcgtc gccgcagacg acgtcgtcga
     3421 gcctggtcac gaggccggca ccggtgcgta ctgctgcacc gcgtgtggcg ccgcgatcct
     3481 cctcgacccg ctcctcgagc ggcaacgcat gcagtcgggc gcggccgagc acgacgtcgc
     3541 ctgacgccgg gcaccgcggt tgcccccgtc ggtacattcg tcgacgtgag tttcgacgtc
     3601 tcggccgatg cctatgcccg gttcatgggc ggtttctccg agccgctggc gccgcagttc
     3661 gccgatcgag ccgaggtccg ggctgggcac cgggtgctcg acgtgggctg cgggccgggt
     3721 gcgttgaccg ccgtactcgt tgatcggctc ggtaacgacc acgtcaccgc cgtcgacccg
     3781 tcggcgtcct tcgtcgaagc gacccgaagg cgccttcccg gcgtcgagat ccgttccgcc
     3841 gtcgcggagg acctgccgtt cgacgacgac acgttcgacc gggccgtggc gcagctggtc
     3901 gtacacttca tgacggaccc tgtcgcgggc atcgcggaga tggcgcgggt gacgcgccct
     3961 ggcggcgtgg tggcggcctg cgtgtgggac cacgggggcg gtaccggccc gttgtcgccg
     4021 ttctggcggg tcgtgcacga cctcgatccc gacgccttcg acgagacgca gctcgcgggt
     4081 gcccgcgagg gccacctcgt cgagctcttc acgaccgcgg gcctcgatgc gccagcgcag
     4141 agcagactca cggtcgagcg tcactacggg gacttcgacg agtggtggga accgttcacg
     4201 tacggcgtcg gaccggccgg gggctacgtc gatggcctcg acgtggacgc gcgggaacga
     4261 ctgcgacagc acgccgagga cgagcttccg agcggtgcgt tcaacctgac ggcatcagcg
     4321 tggtgtgcca ccgccgtcgt ctgaatcgga cgacgcgagg tcagcccatg tgcggatagc
     4381 ggtagctcgt gggcggcgcg aacgtctcct tgatcgatcg cggtgaggtc caccgcatga
     4441 ggttctgggc agctccggcc ttgtcgttcg tacccgacgc gcgagcgccg ccgaacggct
     4501 gctgtccgac caccgcaccg gtgggtttgt cgttgacgta gaagttcccg gccgcgaacc
     4561 gcagcttctc catcgccgct acgatcaccc gacggtcctg cgcgatgacc gctcccgtca
     4621 acgcgtacgg cgcgaacgac tccatctggt cgacggcctt gtcgtagtcg gcgtcgtcgt
     4681 acacgtgcac cgacaggatc gggccgaagt actccgtctt gaacatcgcg tcggtcgggt
     4741 cgctggactc gaggacggtg gggcggacga aatagccgac ggcaccgtcg gtcgtgcccc
     4801 cggtcagcac ctgagtcctc gaggaccgct tgacccgtgc gatcgccttc ttgtgcttgt
     4861 cgaacgcccg cgcgtcgatg acggcaccca tgaagttcga gaagtcggtg acatcgccca
     4921 tcgtcaacga atcgacctcc gccaggaagt ccttccgcac cttgcgccag accgacctgg
     4981 cgacgtacgc acgcgatgcc gcggagcact tctgcccctg gtactcgaat gcgccccgga
     5041 tcagcgcggt gcgcaagacg tcgtggtcgg cggacggatg cgcgacgacg aagtcctttc
     5101 ctccggtctc accgacgatg cgcgggtacg agcggtacga cgagatgttc gcgccgacgg
     5161 tccgccacag gtgctggaac gtcgccgtcg aaccggtgaa gtggatcccg gccaggtcgg
     5221 gatgggtgag cgccgcctcc gacacagcga cgccgtcgcc tggcagcatg ttgatcacgc
     5281 ccggcggaat cccggccgcc tccagcaacc gcatgacgaa gtgcgcggcg aactgctgcg
     5341 tcggggacgg cttccagatc acggtgttgc c
//
LOCUS       DATBFZ010000320         8076 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_4152871, whole genome shotgun sequence.
ACCESSION   DATBFZ010000320 DATBFZ010000000
VERSION     DATBFZ010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 8076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8076
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4152871"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="VH419_09915"
     CDS             complement(<1..546)
                     /locus_tag="VH419_09915"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY7043970.1"
                     /translation="MTRDAESIEFRHTSAEAGVASPLGVDWIHGSVSSKHNTDPDDQV
                     HWYDEHTVILRQNKAINYEAPFMFLLFGGSSAVLIDTGATESPEFFPLRTVIDGLMTQ
                     WLTRYPRDDYRLVVLHSHAHHDHTAGDGQFADRWGTTVVAAQLDVVAGYLGLTSDLDQ
                     PARLDLGGRTLDCIASPGHDAA"
     gene            complement(583..2016)
                     /locus_tag="VH419_09920"
     CDS             complement(583..2016)
                     /locus_tag="VH419_09920"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY7043971.1"
                     /translation="MSTNTPDTNLEASRPTGRERAGEFSLDVEERSIGDAFKGWIDRL
                     RSGDPGALPSVLGLVVLALIFTQVSDQFLSKFNIGNIPGQGLYIAIIALGLVFVLLLG
                     EIDLSAGTAGGTCAAIAAQAVFSGNLHEALPTALYWGLVVGLVVAIGLAVWLKAWTGA
                     VVVAIGLALVATNLTQHLFLALVAAVCIGAAIGVLTGYMVAKVGIPSFVVTLALFLAW
                     QGVLLFSLNAQPINTSNYELWHNLTYGNLSPAWSWVFSIVVVAGYIAFTLIKSLTAKR
                     AGLARDSLSLVLLRGGVLAAVAIVLTYLANQNRNTNPYKVIAGIPWAAVIAITLMIAC
                     TIMLTKTTWGRHLYATGGNAEAARRAGIDVVHMKLTAFILCSSFGALGGIALASSQSS
                     ASLDLGSGNVLLFSVAAAVIGGTSLFGGRGKPRDAIIGALVITIIPNGLGLKPSLGPQ
                     WQQVITGVVLLLAAAVDAISRRRSARG"
     gene            complement(2026..2805)
                     /locus_tag="VH419_09925"
     CDS             complement(2026..2805)
                     /locus_tag="VH419_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020386260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY7043972.1"
                     /translation="MAEPILASRKLNKSFGPVHVLHDVDFDVYPGEVTALVGDNGAGK
                     STLVKCFAGINSIDSGELLFEGKPVVIHDPRAATDLGIEFVYQDLALADNLDITQNMF
                     LGRESRRLNTFLADGEMEKRARETLTSLSVRTVSSVRQLVTNLSGGQRQTVAIAKAVL
                     WNGKVVFLDEPTAALGVAQTRQVLDLVRRLADQGRGVVLISHNMNDVMEVADRVCALY
                     LGRVAAEVPKRETNTTQIVELITSGRSGDVGLAAATVNESI"
     gene            complement(2890..4101)
                     /locus_tag="VH419_09930"
     CDS             complement(2890..4101)
                     /locus_tag="VH419_09930"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEY7043973.1"
                     /translation="MSSFIRRFAVTGAAVALSAGTLAACGGDDDSGDGGDGGSSSQEI
                     SAACKLDNPPTSAAESPPEGSPGKASGKVGVILPDTTSSTRYTLYDAPLLQKSLSAAG
                     LTPDVQNAQGDKNKFASIAQNMIGGGAKVLIIDSIDAASGAGVEKAADQAGVQVIDYD
                     RPNLGGTAPYYVSFDNEEVGKLQAQTMIDCLTEQGVDNPNIIMMNGGTDVDNNAVLFQ
                     KGAHEVLDPLVDAGKATITQEATVKGWNIEDAAPTFQQALTAAGGTVDGVVAANDDIA
                     NAVIGVLKNSGLNGHVVITGQDSGVEGLQNIITGQQSMTVFKNVTLEADAASRLAIAL
                     AAGKDPSSSGLKLEPFDDPQAPEHKLQAVLLPTQVITQANVQDVIDAGALKADEICKG
                     IEDDCQKLGIS"
     gene            4269..5456
                     /locus_tag="VH419_09935"
     CDS             4269..5456
                     /locus_tag="VH419_09935"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEY7043974.1"
                     /translation="MPTTVPAPGSTASLRTANQHRVLEVLRRHATEPKQSAESRLTQA
                     DLARMTGLAPATVSNIVREFAAAGLVDAEPGSGRRGTSVRLAARVGLVSGIDFGHSHV
                     SVAVGDLTGRVLSEEWQSLDPSHRHEEGLAYAADMLDGQLVGLGIDRSVLRTVGLGLP
                     APVIDEVVRSSTILPGWVGVNARRVAEQQLACPVHIENDANLGALAEHRVGVARGHRT
                     SIFLKLSSGVGSGIIIDDHLFHGAGGTAGEVGHLTVDEHGPVCRCGSRGCLEAYASTG
                     SVLELMATHMPGATLDEIVTAAEAGNASALRSLEDAGFHLGWGIGSLVNILNPSLVVV
                     GGDLARAGDLLLDPARVGLRRHALDAVASTPVVVSKLGHRASLVGAVLLAAERTELLS
                     AVD"
     gene            complement(5464..5919)
                     /locus_tag="VH419_09940"
     CDS             complement(5464..5919)
                     /locus_tag="VH419_09940"
                     /inference="COORDINATES: protein motif:HMM:NF021443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LiaF domain-containing protein"
                     /protein_id="HEY7043975.1"
                     /translation="MSHAAGTRRRKLLRALFLVVVAGVVSQLVSKRMTSGDEDSDAFQ
                     LAAVFGGREFKSRATALRSASALAVMGGVEIDLREATLDPAGATLDITTVMGGVEVTL
                     PPGWVVDLETHEHMGGVDSRLTDSADTAEGAPTLHVKANAWLGGVEIRQ"
     gene            6159..7652
                     /locus_tag="VH419_09945"
     CDS             6159..7652
                     /locus_tag="VH419_09945"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEY7043976.1"
                     /translation="MVRLLGGGVLQVATPDDLSSVNVNELTGGGVISVTDFAHPLEAV
                     APCEVHERPPGAAEVLCPVDQVSSVLILTGQGNDRVTTQVAVPVFVLTGAGDDHAFDD
                     HSPEAQFLMGPGDDEVVPGSGANDVQGGEGTDHVRYDDATDAVAVSLDDLANDGAGPR
                     DDNIHSDVEDIVGGFGDDLLVGSDDANVFDGREGDDTILGQGGDDTVATIFLNSINGA
                     DAIFGGSGIDTVSYADRFAGGGVAVRLDDLPFDGQAGENDNVHSDVENIIGSTNPDVL
                     TGSAAGNVIDGLEGNDLLDGGLGPDLVDGGAGTDSISYADRTTAVTVRLDDAADDGQA
                     GEGDNILETENVTGGDGNDLLVGDLFRNLLSGGPGNDTLRGLGQDDVLSGGPGRDLTD
                     AGSGSDTVTYIDHQAPVTASLDGVANDGQPGERDNVVAAENIIGGNGNDTLVGNADRN
                     VLRGLLGDDRLLGRAGDDTLIGSEGTDFADGGAGLDHCQTESSVRCP"
BASE COUNT         1349 a         2643 c         2730 g         1354 t
ORIGIN      
        1 ggccgcgtcg tgaccgggac tggcgatgca gtccaacgtc cgcccaccga ggtccagtcg
       61 ggccggttgg tccaggtcag acgtgagtcc caggtagccc gccacgacat ccagctgggc
      121 ggccacgact gtcgttcccc agcggtcggc gaactgcccg tcgccggcag tgtggtcatg
      181 gtgcgcgtga ctgtgtagga cgacgagccg gtagtcatcg cggggatatc gtgtcaacca
      241 ctgcgtcatc agcccgtcga tcaccgttcg cagggggaag aactcggggg actcggtggc
      301 cccggtgtcg atcaggactg cgctggaacc gccgaaaagc aagaacatga acggcgcctc
      361 gtagttgatc gccttgttct ggcgcaggat caccgtgtgt tcgtcgtacc agtggacctg
      421 gtcgtcgggg tcggtgttgt gcttcgacga gaccgagccg tgtatccagt cgacgcccag
      481 cgggctcgcc accccggcct cggccgacgt gtgcctgaat tcgatcgact ctgcatcgcg
      541 tgtcatgcga tcagccattg ctgcgacacg gcggcaccgt acctagcctc gcgctgatct
      601 ccgccgggag atggcgtcga ccgccgcggc caggagcagc acgacgccgg tgatgacctg
      661 ttgccactgc ggaccgagac tcggcttcag gccgaggccg ttcgggatga tcgtgatgac
      721 aagggcgccg atgatggcgt cacggggctt gccacgaccg ccgaacagcg acgtgcctcc
      781 gatgactgcc gccgcgaccg agaacagcag gacgttgccg gagccgagat cgagcgaggc
      841 agacgactgt gacgaggcca gcgcgatacc gccgagcgcg ccgaacgacg agcacaggat
      901 gaaggccgtg agcttcatgt gtacgacgtc gatgccggct cggcgagcag cttcggcgtt
      961 gccgccggtg gcgtacagat gacggcccca ggtcgtcttc gtcagcatga tcgtgcaggc
     1021 gatcatcaag gtgatcgcga tgacagctgc ccagggaata ccggcgatga ctttgtaggg
     1081 gttggtgttg cggttctggt tcgccaggta ggtcagcacg atggccaccg ccgccaacac
     1141 tccgccacga agcagcacca ggctgaggga gtcgcgggcg aggcccgccc gcttggcggt
     1201 cagcgacttg atcagggtga aggcgatgta ccccgcgacc acgacgatcg agaagaccca
     1261 gctccacgcc gggctcaggt tgccgtacgt caggttgtgc cacagctcgt agttggacgt
     1321 gttgatcggc tgggcgttca gcgagaagag cagcacacct tgccaggcga ggaagagagc
     1381 caaggtgacg acgaagctgg ggatgccgac cttggccacc atgtagccgg tcagtacgcc
     1441 gatggcggcg ccgatgcaga cagcggccac gagcgcgagg aacaagtgct gggtcaggtt
     1501 tgtcgccacc agggcgaggc cgatcgccac cacgacggct ccggtccatg ccttcagcca
     1561 cacagcgagc ccgatggcga ctacgagacc gaccacgagc ccccagtaca gggccgtggg
     1621 gagcgcttcg tggagattgc cggagaagac ggcctgggca gcgatcgccg cgcacgtgcc
     1681 accggcggtg ccggcggaga ggtcgatctc accgagcagc aggacgaaga caaggcccag
     1741 agcgatgatg gcgatgtaca ggccctggcc gggaatgttg ccgatattga acttggacag
     1801 gaactgatcg ctcacctggg tgaagatgag ggcaagaaca acgagcccga gcaccgacgg
     1861 caaggcacct ggatcaccag accggagccg gtcgatccac cccttgaagg catcgccgat
     1921 ggagcgttcc tcgacgtcga gcgagaactc tccagcacgt tcgcgcccag tcggtctgct
     1981 cgcttcgagg tttgtgtccg gggtgttcgt cgacatgccg ggcccctaga tgctctcgtt
     2041 gacggtggcc gcagccagcc cgacgtcacc actacgtccc gaagtgatga gctccacgat
     2101 ctgggtggtg ttggtctcgc gcttcgggac ctccgcggct acccggccca ggtagagcgc
     2161 gcaaacgcgg tcagcaacct ccatgacgtc gttcatgttg tgggagatca ggacaactcc
     2221 gcggccctgg tctgccaggc gccgtaccag gtcgaggacc tggcgggtct gcgccacccc
     2281 cagggccgcc gtgggctcgt ccaagaagac aactttgccg ttccacagca cggccttggc
     2341 gatggcgacg gtctggcgtt gcccacccga caggttcgtc acaagttgtc ggaccgagga
     2401 gaccgtacgc actgacaggc tggtcagggt ctcccgcgct ctcttctcca tctcgccgtc
     2461 ggccaagaac gtgttgaggc gccgggactc ccggccgaga aacatgttct gggtgatgtc
     2521 gaggttgtcg gccagcgcga ggtcctggta gacgaactcg atgccgagat cggtggcggc
     2581 acgaggatcg tggatcacca ccggcttgcc ttcgaagagc agctctccgc tgtcgatgct
     2641 gttgatgccg gcgaaacact tcaccagcgt cgacttgccg gcaccgttgt cgcccacgag
     2701 tgcggtgacc tcaccggggt agacgtcgaa gtccacgtcg tggagcacgt gaacgggtcc
     2761 gaagctcttg ttgagctttc gcgatgcgag gatgggttcg gccacggcgt ccgccttcag
     2821 ttcaggagag ggccagttcg atggtggccg aaccggacgc cggcccggcc accatcggga
     2881 agtcgggcgt caggagatcc cgagcttctg acagtcgtcc tcgatcccct tgcagatctc
     2941 gtcagccttc agcgcgccgg cgtcgatcac gtcctgcaca ttggcctggg tgatgacctg
     3001 ggtcggcagg agcaccgcct ggagcttgtg ctcgggcgcc tgtgggtcgt cgaacggttc
     3061 cagcttgaga ccggagctgg acgggtcctt gcccgcagcc agggcgatcg cgagcctgga
     3121 agccgcgtca gcctccagcg tgacgttctt gaacaccgtc atgctctgct ggccggtgat
     3181 gatgttctgc agaccttcga caccggagtc ctgacccgtg atgaccacgt gaccgttgag
     3241 gcccgagttc ttcagcacgc cgatgaccgc gttggcgatg tcgtcgttgg ctgcgacgac
     3301 tccgtcaacc gtgccgccgg ctgctgtgag tgcctgctgg aacgtcggcg ccgcgtcctc
     3361 gatgttccaa cccttgacgg ttgcctcttg cgtgatggtc gccttgccgg cgtcgacgag
     3421 cgggtcgagg acctcgtggg cacccttctg gaacagcacg gcgttgttgt cgacgtcagt
     3481 gccgccgttc atcatgatga tgttcgggtt gtccacgccc tgctcggtca ggcagtcgat
     3541 catggtctgg gcctggagct tgccgacctc ctcgttgtcg aaggacacgt agtacggggc
     3601 tgtgccgccg aggttcggcc ggtcgtagtc gatgacctgc acgcccgcct ggtccgcggc
     3661 cttctcgaca ccggcacccg acgcggcgtc gatggagtcg atgatcagga ccttcgcacc
     3721 gccaccgatc atgttctgcg cgatcgacgc gaacttgttc ttgtcgccct gggcgttctg
     3781 gacgtcggga gtcagcccgg cagcggacaa cgacttctgc agcagtgggg cgtcgtacag
     3841 cgtgtagcgc gtcgacgagg tcgtgtccgg caggatcacg ccaaccttgc cgctggcctt
     3901 gccgggtgag ccctcaggcg gcgactcggc tgcgctcgtc ggcgggttgt cgagcttgca
     3961 ggcggcgctg atctcctggc tcgacgagcc accatcacca ccatcaccgc tgtcgtcgtc
     4021 gccgccgcag gccgcgagcg tgcctgcgct gagcgctacc gcggcaccgg tcacggcgaa
     4081 gcggcggatg aatgaagaca cgattccctc cctgaacagt ggagcccgag catacgaatg
     4141 cctgcctcga agtgtggtgg gtcgcacgat tacggtcaag agtcgaacgc taagaacagg
     4201 cctgttgcag gagttgccgt tgttcatcat gccttgacgg ttgaaccccc ttcccgcaca
     4261 gtctttccat gcccaccaca gtgccggccc cgggctcgac ggcatccttg cgtacggcga
     4321 accagcatcg cgtcctcgag gtgttgcggc ggcacgcgac cgagcccaag cagtctgctg
     4381 agtcccgtct cacgcaggcg gacctggcac ggatgaccgg tctcgcaccg gccacggtct
     4441 cgaacatcgt gcgcgagttc gctgccgcgg gtctcgtgga tgccgagccc gggagcgggc
     4501 ggcgcggtac gtcggtgcgg ctggctgccc gggtgggtct cgtgagcggg atcgacttcg
     4561 gccacagcca cgtgtctgtc gccgtcgggg acctcaccgg ccgggtgctc agcgaggagt
     4621 ggcagtccct cgacccgtct caccggcatg aagaagggct ggcctacgcg gcggacatgc
     4681 tcgatggcca actcgtcggc ctcggtatcg acaggtccgt gctgcgaacg gtcggtctgg
     4741 gcctgcctgc gccggtgatc gacgaggtcg tgcggtcgtc cacgatcctg ccgggctggg
     4801 tgggtgtgaa cgctcgccgg gtcgccgagc aacagctcgc atgtccagtc cacatcgaga
     4861 acgacgcgaa cctcggtgcg ttggccgagc accgcgtggg cgtggctcgc ggccaccgca
     4921 cctcgatctt cctcaagctc tcctctggtg tcggctcggg gatcatcatc gacgaccacc
     4981 tcttccacgg tgccggcggt accgcaggcg aggtgggaca cctcacggtc gacgagcatg
     5041 gtccggtgtg tcgctgcggc agccgcgggt gcctcgaggc ctatgcgtcg acaggcagcg
     5101 tcctcgagct gatggcgacc cacatgccgg gggcgacact cgacgagatc gtcaccgccg
     5161 ccgaggccgg caacgcgtca gccttgcgca gcctcgagga cgccggcttc cacctcggct
     5221 ggggcatcgg cagcctggtc aacatcctga acccctccct tgtcgtcgtg ggcggtgatc
     5281 tggcgcgagc cggcgacctg ctcctcgacc ccgctcgagt gggacttcga cgacacgccc
     5341 tcgacgcagt cgcgtcgaca ccggtcgtgg tgagcaagct cggtcaccga gcctcgctcg
     5401 tcggcgccgt gctgctcgcc gccgagcgga cggagctgct ctccgcggtc gactgaccac
     5461 gctttactgt cggatctcga ccccaccgag ccaggcgttg gctttcacgt ggagggtggg
     5521 agcaccctcg gcggtgtccg ctgagtcggt cagacgcgag tcgacgccac ccatgtgttc
     5581 gtgagtctcc aggtcgacca cccagccggg tggcagcgtc acctcgacgc cgcccatgac
     5641 ggtcgtgatg tcgagggtcg cacccgcggg gtcgagcgtg gcctctcgga ggtcgatctc
     5701 gacaccaccc atcaccgcca gggccgacgc ggatcgcagc gctgtcgccc ggctcttgaa
     5761 ctctctacct ccgaaaaccg ccgcaagctg gaaggcgtcg gagtcctcgt cgccgctcgt
     5821 catccgcttg gagacgagct ggctgaccac gcccgccacc acgacgagga acagcgcacg
     5881 tagaagtttt cgccttcggg tacccgctgc atgtgacatg cagcgattgt tccactcccc
     5941 tcgtcggggc gagtggatgc atcgggctcg tcacctccct gatgtacgtc gtagcgccgt
     6001 cgcgactcct gctcgatagc gaccgagctg aaggagacga cgtgatcaaa gcgccaacag
     6061 cggccgtgag cctggtggtc gtcacttgca tgtcgggctt gggcactgat gctgctgctg
     6121 cggagacagc cgcaactccc acgtctgacg agccgacagt ggtgaggctg ttgggcggtg
     6181 gcgtgttgca ggtcgccaca cccgacgacc tgagctcggt caatgtgaac gagctcaccg
     6241 gaggtggcgt gatctcggtg acggactttg cgcacccgtt ggaagcggtg gccccctgcg
     6301 aagtccacga gcggccgccg ggtgcagccg aggtgctgtg cccggtcgac caggtcagct
     6361 ccgtgctgat cctgaccggg cagggaaacg accgcgtcac gacgcaggtg gcggtgcctg
     6421 tcttcgtgct caccggcgcc ggtgacgacc atgccttcga cgaccattcc ccggaggctc
     6481 agttcctcat gggacccggg gacgacgagg tggtgccggg atcgggcgcc aacgacgtac
     6541 agggaggcga ggggacagac cacgtgaggt acgacgacgc aactgacgcc gtcgcggtga
     6601 gcctggacga cctggcgaac gacggtgcag gtcccaggga cgacaacatc cactccgacg
     6661 tggaagacat cgtcggcgga ttcggcgacg acctgctggt gggttcggac gacgccaacg
     6721 tcttcgacgg tcgcgagggt gacgacacca tcctggggca gggaggcgac gacacggttg
     6781 ccaccatctt cctgaactcg atcaacggtg cggatgcgat cttcggaggg tccgggatcg
     6841 acaccgtgtc ctacgccgat cgctttgccg gcgggggcgt ggcggtacgg ctcgacgacc
     6901 tcccgttcga tggtcaagca ggcgagaacg acaacgtgca ctccgatgtg gagaacatca
     6961 tcggctcgac gaaccccgac gtgttgaccg gcagcgctgc cggcaacgtg atcgacggac
     7021 tggaaggcaa cgacctgctc gacggcggtc tcggcccgga cctggtcgac ggcggagccg
     7081 gcaccgactc gatcagctac gccgaccgca cgaccgcggt cacggtccgc ctcgacgacg
     7141 ccgccgacga cggccaggca ggcgagggtg acaacatcct cgagaccgag aacgtcaccg
     7201 gcggggacgg caacgacctg cttgtcgggg acctgttccg caacctgctg tccggtggcc
     7261 ccggcaacga cacgctacgt ggactcggcc aggacgacgt gttgtccggc gggccgggcc
     7321 gcgacctcac cgacgccggc tccggatccg acaccgtcac ctacatcgac caccaggccc
     7381 cggtgaccgc cagcctggac ggcgtcgcca acgacggcca gcccggtgag cgcgacaacg
     7441 tggtggccgc cgagaacatc atcggcggca acggtaacga cacgctggtc ggcaacgccg
     7501 accgcaacgt gctgcgcggc ctgctcggcg atgaccggct gctcggccgc gccggcgacg
     7561 acaccctgat cggcagcgaa ggcaccgact tcgccgacgg cggcgcgggc ctcgaccact
     7621 gccaaaccga gtcaagtgtc cgctgtccct gatggtagat ctggggactc ctcggcctcc
     7681 gtgggccgcg cctgctgccg gtgcattcct caggcgtcaa gggtcgcttc gcgatcgctg
     7741 acgcgacgga ctccgtccgc ccttgacccc tgagcctcga cgcaccggtt cgggcaggcg
     7801 ataacgggca ggactgcgtc ccgcccgtgc accggcgcgg cccacccgcc tccggaaagc
     7861 cacctacgcc acgaatcgga catgtgtcgg tggtggtttg cctgggtagc catccaaacg
     7921 gacgggtagc caggcagacc tgccttggca gaggtgggtc tgcctggtga ccgaggcaaa
     7981 ccgggagccg gtcaagggcc cttcacgacc gtctccgccc cacagaaact gaggcgtccc
     8041 caagagtgct ctgtgcgcga ggggggagtt gaaccc
//
LOCUS       DATBFZ010000321         5788 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_2189213, whole genome shotgun sequence.
ACCESSION   DATBFZ010000321 DATBFZ010000000
VERSION     DATBFZ010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 5788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5788
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2189213"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            176..1270
                     /locus_tag="VH419_09950"
     CDS             176..1270
                     /locus_tag="VH419_09950"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008361348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY7043977.1"
                     /translation="MSIAEQHDDAEPIADAFAERVFASALGAVELFSVYVGDRLGWYR
                     SLADEGPATYAELAARTSTDERYTQEWLEQQAVYGILETDPSVAADERRYGMNEAVTE
                     VLTDETSQAYLAPISRAFAAVGKQLPALLDAYRSGGGVSWDDLGDDAREAQAAMNRPW
                     FEQQLGGALSGVPEVHEILSRDDARILDVGAGGGWSSIALAKAYPNARVDGIDVDAPS
                     VEMATQNAADAGLSDRVSFGLGDAGSLGDSAAYDAAFAFECVHDMPRPVEVLAAVRGA
                     VKPDGAVIVMDEAVADEFTAPGDDVERLMYGFSVFVCLPDGMSSQPSVGTGTVMRRST
                     LERYADEAGYAGVDVLPIEDFAFFRFYRLR"
     gene            complement(1335..2114)
                     /locus_tag="VH419_09955"
     CDS             complement(1335..2114)
                     /locus_tag="VH419_09955"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY7043978.1"
                     /translation="MSEREHYDPGLPCWIDLGTPDVDAAAAFYSGLFGWDFEETSPAS
                     GDYRMAVLKGRRVAGLGPAQAEGVPWWTTYFSVNDASAAAAATEANGGGVIDPAQDVA
                     GVGSMAVLADPEGATFSVWQPGAHFGAGLRDEHGTLGWTELMTRDIETARKFYAAVLG
                     WESEDYPMGAFTYTVWNVGESNAAGGMPMSPEQFPDEVPSHWMVYFEVDDCDATAVKV
                     AELGGSIAHPPTDIPQVGRFAVCNGPTGEVFSIIHAVGPSS"
     gene            complement(2250..3104)
                     /locus_tag="VH419_09960"
     CDS             complement(2250..3104)
                     /locus_tag="VH419_09960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043979.1"
                     /translation="MKIDERGRTAGAALRSSTTQDLPVAAHYEQLITTARRHHRLRVA
                     AVAAAVATLIGGAGYALANAPRDADLAPTDQPPATSTPPQSDCDSPYLRCTAGGRITM
                     QMPVPVSWTVGPQFDREPQALVDSGTSQVYLGENYQRDATAGVTVAEGVQPVRATSQA
                     EPASGVPADARSVAQWVADRPFLDASAVQHTTVDGVDAWTVRAATRDPSRQGVARCNG
                     GRFTCMPLLSLTKFPADTLGVWDTMISQYTFVDLPGAGVTMIWSWSFDGERSFVANQQ
                     VVDSIRFK"
     gene            complement(3870..4442)
                     /locus_tag="VH419_09965"
     CDS             complement(3870..4442)
                     /locus_tag="VH419_09965"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043980.1"
                     /translation="MRTINKRRLTGIGVATAAVALGVSGVGSVALAAHVTGTAGNDVL
                     QGTPKVDRIEGRGGDDILRGLRGDDVLLGGSGDDIMVGGAGSGEDLMFGGKGRDQAFG
                     GPSFDALYGGPGADTFFGGPGRDTGDLIGRDHVFLGRGNDWMSLLDNDGVRGFVDCGP
                     GHDVVSYLGSIDMHDRWDNCEVLIAENPGD"
     gene            4917..5447
                     /gene="nrdR"
                     /locus_tag="VH419_09970"
     CDS             4917..5447
                     /gene="nrdR"
                     /locus_tag="VH419_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HEY7043981.1"
                     /translation="MHCPYCRHTDTRVLDSRVAEDGTSIRRRRSCPECEKRFTTVEQM
                     QLTVVKRSGATEPFSREKAIAGVRKACKGRPVSDDDLALLGSQVEDVLRSSGWAEVPA
                     HEVGLAILGPLRELDEVAYLRFASVYRSFESADDFETEIALLRAERQPTGEEPDFVQH
                     PQDPIRRTPSTKEPST"
     gene            5531..>5788
                     /locus_tag="VH419_09975"
     CDS             5531..>5788
                     /locus_tag="VH419_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002523935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043982.1"
                     /translation="MTETDSRRPAQFKRGKGLKIERIFTSPGTHPYDEVTWERRDVVQ
                     QNWKTGETVFEQRGVEFPDFWSVNASTIVTTKYFRGAVGTDA"
BASE COUNT          889 a         2015 c         1977 g          907 t
ORIGIN      
        1 cgcattcgcc tggacggcgt gcgacgtcgc tcatctctgc atcctgccgc aaccgccgcg
       61 ttgtgcggca cctgctccaa tcggtttgca catccgtcgg ggttgacccg cgtcggtaac
      121 ctcgctgaat tccgacgggg agctcgaccg gctgagaccc accagggagg acccgatgtc
      181 catcgccgaa cagcacgacg acgccgaacc gatcgccgac gccttcgcgg aacgcgtgtt
      241 cgcctcagcg ctcggggcgg tggagctctt cagcgtgtac gtcggcgacc ggctcggctg
      301 gtaccgctcg ctcgccgacg agggtccggc gacgtacgcc gagctcgccg cacgcacctc
      361 caccgacgag cgctacacac aggagtggct cgagcagcag gccgtctacg gcatcctcga
      421 gacggacccg tcagtcgcgg cggacgagcg gcggtacggg atgaacgaag ccgtcaccga
      481 ggtgctcacc gacgagacga gccaggcgta cctcgcgccg atctcgcgcg ccttcgcggc
      541 tgtcgggaag cagctcccgg ccctgctgga cgcttatcga tccggcggcg gtgtcagctg
      601 ggacgacctc ggcgacgacg cacgggaggc gcaggcggcg atgaaccggc cgtggttcga
      661 gcagcagctc ggcggcgcct tgagcggggt gcccgaggtg cacgagatct tgagccggga
      721 cgacgccagg atcctcgacg tcggcgccgg cggcggctgg tcgtcgatcg cgttggccaa
      781 ggcctatccg aacgcgcgcg tcgacgggat cgacgtggac gcgccgtcag tggagatggc
      841 tacgcagaac gccgcggacg cggggctgtc cgacagggtc agcttcgggc tgggtgacgc
      901 cggctcgttg ggagactccg cggcgtacga cgccgccttc gcgttcgagt gcgtgcacga
      961 catgccgcga ccggtcgagg tgctcgctgc cgtgcgcggg gcggtgaaac ccgacggtgc
     1021 cgtcatcgtg atggacgagg cggtcgccga cgagttcacc gcgccaggtg acgacgtcga
     1081 gcggctgatg tacgggttca gcgtgttcgt ctgcctgccc gacgggatgt cgtcgcagcc
     1141 gtcggtcggg accgggaccg tgatgcggcg gtcgaccctc gagcggtacg ccgacgaggc
     1201 cgggtacgcc ggtgtcgacg tcctgccgat cgaggacttc gccttcttcc ggttctaccg
     1261 cctccgctga actccgatcc gagccgatcc gacccggcgc gggactgccg cgcgaggtcc
     1321 ggtcgaatcg gttgtcagga cgacggaccg acggcgtgga tgatcgagaa cacctcgccg
     1381 gtcgggccgt tgcagacggc aaaccggccg acctgcggga tgtccgtcgg cgggtgggcg
     1441 atcgagccgc cgagctcggc caccttgacg gccgtggcgt cgcagtcgtc gacctcgaag
     1501 tacaccatcc agtgcgacgg cacctcgtcc gggaactgct ccggtgacat cggcataccg
     1561 ccggcagcgt tcgactcgcc gacgttccac accgtgtagg tgaaggcgcc catcgggtag
     1621 tcctcggact cccagccgag cacggcggcg tagaacttgc gcgcggtctc gatgtcgcgg
     1681 gtcatcagct cggtccagcc gagggtgccg tgctcgtcgc gcagccccgc gccgaagtgg
     1741 gcccccggct gccagaccga gaacgtcgcg ccctccggat cggccagcac cgccatgctt
     1801 ccgacgcccg ccacgtcctg cgccgggtcg atgaccccgc cgccgttcgc ctcggttgcg
     1861 gcggctgccg ctgaggcgtc gttgacggag aagtacgtcg tccaccacgg cacgccctcg
     1921 gcctgcgccg ggccgagacc cgccacccgc cgacccttca gcacggccat ccggtagtcg
     1981 ccgctcgcgg gcgacgtctc ctcgaagtcc cagccgaaca ggccggagta gaacgccgcg
     2041 gccgcgtcga cgtcaggtgt gcccaggtcg atccagcagg ggagtccggg gtcgtagtgc
     2101 tctcgttcgc tcatgctctg aacctaggcc ctcgatttcg accatctcaa tcagcgggcg
     2161 tgcgagcttc ggcggttgag cctgtcgaaa ccccgatgcc cttgatctcg acgagctcga
     2221 tcagcgggta ttccgaccgg cgagttgcgt cacttgaacc ggatcgagtc aacgacctgc
     2281 tggttggcaa cgaacgagcg ctccccgtcg aagctccagg accagatcat cgtcacgccc
     2341 gcccccggga gatcgacgaa cgtgtattgg ctgatcatcg tgtcccagac accgagcgtg
     2401 tccgccggga acttggtcag ggacagcagc ggcatacatg tgaaccggcc accgttgcac
     2461 ctggccactc cttggcgcga cgggtcgcgc gtcgccgctc gcacggtcca ggcgtccacg
     2521 ccgtccactg tggtgtgctg cacggcggag gcgtcgagga acgggcggtc cgcaacccat
     2581 tgcgccaccg accgcgcatc ggccggtacg ccgctcgcag gctcagcctg gctcgtggct
     2641 cgcacgggct ggacgccttc ggccaccgtg acacccgcag tcgcatcgcg ctggtagttc
     2701 tcgccgaggt acacctgtga cgtgccggag tcgacgaggg cttgcggttc acgatcgaac
     2761 tgcggtccga ccgtccacga caccgggacg ggcatctgca tggtgatccg cccacccgcg
     2821 gtgcaccgca agtacggaga gtcgcaatcg ctctgcggcg gagtcgatgt ggccggcggc
     2881 tggtccgtcg gtgccaggtc ggcgtctctc ggggcgttcg ccagcgcata gccggcgccg
     2941 ccgatgaggg tggcgacggc ggctgccacg gcggccactc gcagccgatg gtgtcgtcgg
     3001 gccgtcgtga tgagctgctc gtagtgtgcg gcgaccggca ggtcctgggt ggtggacgac
     3061 cgcagcgcgg caccggcagt acggcctcgc tcgtcgatct tcacgattcg tcctccgtct
     3121 ccatgccgag tcggtcggcg agagttgccg ggcgcgatgg agatgtgcct tgaccgttcc
     3181 tggcgcgcat tccaacgtga gtgcgacgtc ggcgagactg aggtcgaggc cgtagtagag
     3241 cgcgaccacc tgggcctgtc gctgcggcag tcggcgtacc tcctgccaga actcgtcgtc
     3301 gccttcgaga cgcgcttcgg tcgtggccgg gcgacgtgcc cacaggcgag cgaccgccct
     3361 tccctcggac gctcgacggc gtacgtgcga gatcgccttg tgcaggcaca ccccacggac
     3421 ccagccccct ggagacgcga actcccgtac gtcgccccag cggcgatacg cgacgagcat
     3481 cgcctcctgc gcgatgtcgg ccccgttggc aggaccggcg agcgcagctg cgagcgtcac
     3541 catctgtggg aactcgcggt ggtagaactc ctcgaacgtc cccgggagat cgtcgccgcc
     3601 gagctcggca tcggcgcatt cggcccaagc ggcgagggcg gcgtccgcgt cgtcgctggg
     3661 aacagcagcg tccaccagct ggaggacgcg ctgctcacgt cgactcgttc gccaggccat
     3721 gtaccttcat cgagccaggc caggcatctg gtttacctgg cggtccgcct cccgcaccgc
     3781 caccatgctc gctccccggc gaggtgccaa ctttcgtccg tgtttgacgg cgtgtcggac
     3841 gccggtcgac acctcggcgg gcggccggat cagtcgccgg gattctccgc tatcaggacc
     3901 tcgcagttgt cccaccggtc gtgcatgtcg atcgagccga ggtagctcac gacgtcgtgg
     3961 cccggcccgc agtcgacgaa gccccggacg ccgtcgttgt cgagcagact catccagtcg
     4021 ttgccacgac ccaggaagac gtggtcgcgg ccgatgaggt cgccggtgtc ccggcccgga
     4081 ccgccgaaga acgtgtctgc tcccggcccg ccgtacagcg cgtcgaagct cggaccgccg
     4141 aacgcctggt ctcggccctt gccgccgaac atcaggtcct cgcctgagcc agcgccgccg
     4201 accatgatgt cgtcgccgga gccgccgagc agcacgtcgt cgcctcgcag accacgcagg
     4261 atgtcgtctc cgccccggcc ctcgatccgg tccaccttcg gcgtcccttg caggacgtcg
     4321 ttgcccgccg tccccgtgac gtgtgccgcc agcgccacac tgccgacgcc gctgaccccg
     4381 agcgccacgg cggccgtggc gacgccgatg ccggtcagtc gtcgcttgtt gatcgttcgc
     4441 atgatgtccc cccgattgct tgcctcggct cgttgccgat ggggaactga cgcatgagcc
     4501 gcatgttcgg ttgccctgac gctctcaggt gcctgccgac acgccagcgg cgtacgccga
     4561 aaagggcggc tgacctgcga cgatgcggct gcggcgtacc cccgacgcgc cgattgcgcg
     4621 gaattgttgc tcctgcgaac ccttgagcgt agggtcgccc ccagatatag tagttacacg
     4681 gatgtagttc tccacaggtt gtggtttggc ggtcctcttc gtcgtccaca accgcccgag
     4741 gcgggcatcc ggacgactac ccacaaggac accgaggttc atccccagga cgtccacagc
     4801 tcggcccctt cagacgggtt gtccacaggg ccgggactgt cggaccgccg cggtagaacg
     4861 agcggtatga gcgcacggat ctcgccagag atcgagcacg cgaggaggcg gccgacatgc
     4921 attgtccgta ctgccgccac accgacaccc gggtgctcga ctcgcgggtc gccgaggacg
     4981 gtacgtcgat ccgccggcgg cgctcgtgcc cggagtgcga gaagcggttc accacggtgg
     5041 agcagatgca gctcacggtg gtgaagcggt ccggtgcgac cgagccgttc agccgtgaga
     5101 aggcgatcgc cggcgtccgc aaggcgtgca agggccggcc ggtcagcgac gacgacctgg
     5161 cgctgctcgg ctcccaggtc gaggacgtgc tgcggtcgtc cggctgggcg gaggtgcccg
     5221 cccacgaggt cgggctggcc atcctcgggc cgctgcgtga gctcgacgag gtggcctatc
     5281 tgcggttcgc gagtgtctac cgctcgttcg agtccgccga cgacttcgag accgagatcg
     5341 cgctgctgcg cgccgagcgg cagcccaccg gtgaggaacc cgacttcgtg caacacccgc
     5401 aagaccccat tcgacgtacg ccgagcacca aagaaccgag cacctgacga gacgagacga
     5461 gccagcgacc cccggcatca ccacggatgc caccaccacc gcccgaccgg cggagacgag
     5521 gcaggatgac atgaccgaga ccgacagccg gcgccccgcg cagttcaagc gtgggaaggg
     5581 tctgaagatc gagcggatct tcacttcgcc cggcacgcac ccgtacgacg aggtcacgtg
     5641 ggagcgtcgt gatgtcgtgc agcagaactg gaagacgggc gagaccgtct tcgagcagcg
     5701 gggtgtggag ttcccggact tctggtcggt caacgcgtcg acgatcgtca cgacgaagta
     5761 cttccgcggt gcggtcggca ccgacgcc
//
LOCUS       DATBFZ010000322         7975 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_2511445, whole genome shotgun sequence.
ACCESSION   DATBFZ010000322 DATBFZ010000000
VERSION     DATBFZ010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 7975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7975
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2511445"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1216)
                     /gene="dnaB"
                     /locus_tag="VH419_09980"
     CDS             complement(<1..1216)
                     /gene="dnaB"
                     /locus_tag="VH419_09980"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEY7043983.1"
                     /translation="MSIAEIYPPDDDRRGQIGRTPPQDEQAEQSVLGAMLISKDAIAD
                     VIEVVRGPDFYRPGHELIYDAILDLYGRGEPADAITVSAELTRAGDLARVGGAPYLHT
                     LVSSVPLATSAGYYAGIVREKAILRRLIDAGTRIVQFGFAAEGQVDDVVDRAQAEVYA
                     VTEKRTSEDYAPLADIMELTIDELEAIGNRDGAMGGVPTGFTELDDLTNGLHGGQMVI
                     LAARPAVGKSTMGLDIARSASIKHGLASCIFSLEMSRTEITMRLLSAEARIPLNHMRN
                     GKMSDDDWNKLVRKMGEVSEAPLFIDDSPNLTMMEIRAKARRLKQRHDLRLIVLDYLQ
                     LMTSGKKVESRQVEVSEFSRQIKLLAKELDIPVVAIAQLNRGSEQRTSKRPMLADLRE
                     SGCLTADTRIMRA"
     gene            1537..2976
                     /locus_tag="VH419_09985"
     CDS             1537..2976
                     /locus_tag="VH419_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017933524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEY7043984.1"
                     /translation="MGPDRDDLLARPHPGGQVFPTDFHDAPDDTPNELPARTKGHRAQ
                     DKEIARLAIPAFAALISEPLFLLADAAIVGHLGTAQLAALGIAGTVIQTAVGLFVFLA
                     YGTTSTVARSVGAGDRAGALSVGVDGIWLAVIIGTLAATATALLTPTIVSGFGAADDV
                     DGYTTTYLRIAAFGIPSLLLLLAATGVLRGLQDTRTPLYVAVVANLANIALNVTFVYG
                     FGWGIAGSAAGTLVAQSVAALAIVAVVVRAARRVGAQLVPHLPGVAAAWRTGVPLIVR
                     TLSLRLALLLATYVAAAISTPAVAAHQVAFTIWMFLAFALDAIAIAGQAITGKLLGAG
                     DAVAARAATRRMMQWGLVSGIALGVGLALIRPLIVPLFTSDAQVQDLLSLVLLIAAAQ
                     QPVSGIVFVLDGVLIGAGDGRFLAWAGVVNLLVFVPLALAVPWLGGGIGWLWGAFTAF
                     MLMRLVTLLWRERSDSWLVLGAAPAARNR"
     gene            complement(3069..3521)
                     /gene="rplI"
                     /locus_tag="VH419_09990"
     CDS             complement(3069..3521)
                     /gene="rplI"
                     /locus_tag="VH419_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020574657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEY7043985.1"
                     /translation="MKLILTQEVSNLGAPGDVVDVKDGYGRNYLLPRGAAIRWTRGAE
                     KQIDSIKKAREIRDIRDVSHADEIKAQLEDLTVRVQAQAGSSGRLFGAVTLSDIVDAV
                     RAAGGPDIDKRRIQVGNPIKTLGAHEVIVKVHDEVSATVKLNVVPTAS"
     gene            complement(3536..3772)
                     /gene="rpsR"
                     /locus_tag="VH419_09995"
     CDS             complement(3536..3772)
                     /gene="rpsR"
                     /locus_tag="VH419_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003949403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEY7043986.1"
                     /translation="MAKPVVRKPKKKVCAFCKDKATYVDYKDTTLLRKFISDRGKIRA
                     RRVTGNCTQHQRDVATAIKNAREMALLPYTSTAR"
     gene            complement(3838..4362)
                     /locus_tag="VH419_10000"
     CDS             complement(3838..4362)
                     /locus_tag="VH419_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011758086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEY7043987.1"
                     /translation="MAGETSITIVGNLVDDPDLRFTPSGAAVANFRIASTPRTFDRQT
                     SEWKDGETLFMTCAVWRQAAENVAESLQRGMRVIVQGRLKARSFETREGEKRTVFEID
                     VDEVGPSLRSATAKVTRASRSGAEGGSSAGGVSQGGSDPWATPAPAAGAHAGQPANDP
                     WASPSASSSDEPPF"
     gene            complement(4475..4765)
                     /gene="rpsF"
                     /locus_tag="VH419_10005"
     CDS             complement(4475..4765)
                     /gene="rpsF"
                     /locus_tag="VH419_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020134133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEY7043988.1"
                     /translation="MRHYELMVILDPELEERTVQPSLDQYLNLVRQDGGSVESVDVWG
                     RRRLAYEINKKSEGIYAVVTLSAEPGTVKELDRQLGLNESVMRTKVLRPEAH"
     gene            complement(5434..>7975)
                     /locus_tag="VH419_10010"
     CDS             complement(5434..>7975)
                     /locus_tag="VH419_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEY7043989.1"
                     /translation="VTWRNLMARKIRLALSGFAIVLGVAFVAGSFIFTDALGNAFNGI
                     IKGTTADVEVAPKGANDFTSFGSDARTIPASVVPKLAALPEAAAADPVIQVDGVYVIS
                     DKGKLVGGNGPPGLAFNFNETTAITGDQILTLEKGELPQGPAQIALDEGTADKAGYQI
                     GDDVKLVTPGRNPTMTATLTGLVKFGSEGGLVGATLTIFDERTLQDLFFDGRDVVTSI
                     SLTAADGVSQEQLADAAQPSLPPGVTAEPGDKVAKDNEDAIGKALGFINTFLLVFAAV
                     SMVVGTFLIINTFSILVAQRSRELALLRAMGASKRQVNRSVLAEAFVVGLIGSIIGIA
                     VGYLLAIGLKALFATFGLDLGSASLPVEPRTIVVSLVVGLVVTMLAAYLPARRAGRIA
                     PVAAMRDDVALPQATLRRRLIVGVAMMVVGVGLMVLGFTAEGSRGLLLIGIGMLVILI
                     GAALTSPVVGRPVIVGLGALYRRVFGTVGTLAKENSLRNPRRTAATASALMIGLTLVA
                     LMSILGQSAKASTDKAIDSSLNAQLIVSNAVSQSFSPSIAERIRQVDGVASVAQFREA
                     GPKVDGEQVFVGATDPRELAQALDLPMEFGGLTELSDDSVLVSSNAAKSQNLSLGDTV
                     AMKFPARTENLKVAGIYPSGGVVFTDYLVSLDTMQRGGYAPRDSLLYISQEPGASEAS
                     IRAGIDEILAGNPTVTLKNQQEYADEQKQQIDQFLAIIYALLGLAVVIAVLGIINTLA
                     LSVIERTREVGLLRAVGLSRRQLRRMVRLESVAIAVLGAVLGVVLGIGFGVALQRAIS
                     DQGVDVLSIPWVQLVVFVLISAVVGVLAAVLPARRAAKLDVLEAITTE"
BASE COUNT         1227 a         2655 c         2708 g         1385 t
ORIGIN      
        1 cggcccgcat gatgcgagtg tctgcggtca gacatcccga ctcacgaagg tcggcgagca
       61 tgggtcgctt cgacgtgcgc tgctccgatc cacggttgag ctgagcgatc gcgacgacgg
      121 ggatgtcgag ctccttggcg agcagcttga tctgccggga gaactccgac acctcgacct
      181 ggcgggactc caccttcttg ccggatgtca tcagctgcag gtagtcgagc acgatcagtc
      241 gcaggtcatg acgctgcttg aggcggcgcg ccttcgcccg gatctccatc atcgtgaggt
      301 tcggtgagtc gtcgatgaac agcggcgcct cggacacctc gcccatcttg cggacgagct
      361 tgttccagtc gtcgtcgctc atcttgccgt tgcgcatgtg gttgagcgga atgcgtgcct
      421 cggccgacag cagccgcatg gtgatctccg tgcggctcat ctcgagagag aagatgcaac
      481 tggcaagacc atgcttgatc gacgccgaac gagcaatgtc gagtcccatg gtcgacttcc
      541 cgaccgcggg tctcgccgca aggatcacca tctggccgcc gtggagtccg ttggtgaggt
      601 cgtcgagctc ggtgaacccg gtcggtacgc cgcccatggc gccgtcgcgg ttgccgatcg
      661 cctcgagctc gtcgatggtg agctccatga tgtcggcgag cggcgcatag tcctccgacg
      721 tacgcttctc ggtcaccgca tagacctcgg cttgcgcacg gtcgacgacg tcgtcgacct
      781 gcccctcggc ggcgaacccg aactggacga tccgggtgcc ggcgtcgatc agtctccgga
      841 ggatggcctt ctcccggacg atcccggcgt agtagccggc gctggtcgcg agcgggaccg
      901 aggagaccag ggtgtgcagg tagggggccc caccaacgcg agcgaggtcg cctgcccggg
      961 tcagttcggc ggagaccgtg atcgcgtcgg caggctcacc acgcccgtag aggtcgagga
     1021 tcgcgtcgta gatcagctca tggcccgggc ggtagaaatc gggaccccgg acgacctcga
     1081 tgacgtcggc gatcgcgtcc ttgctgatca gcatcgcgcc gagcacggac tgttcggcct
     1141 gctcgtcctg cggcggcgtc cgtccgatct gtccgcgacg gtcgtcgtcg ggcggataga
     1201 tctctgcgat gctcacgaag cccctctcaa cccgaccgtc ccggcgggcc tcgtcggcgt
     1261 tgaacgagcc ctcgtctgac aaagtagggg ggtgcgccga cagtcccggc aggcgacgtt
     1321 acgacggcgt cgggatcggt ggaaggtctc ggtcaacagc ccgctgtgga catcccgcgg
     1381 aagttgtgga aaacaccggc cgcgcgtgtg cacaggctgg ggaaaggtcc gtggacagcc
     1441 ttgtcccggc ggggtcgaca cgccgtctga cctggacaaa cgggagttcg cagtgtgtgg
     1501 aggagagaaa gtttggaccc ggtgtcaggg ggtgagatgg gtcccgatcg ggacgatcta
     1561 ttagcacgtc cgcatcccgg tggccaggtg tttcccacag attttcacga cgcgccggac
     1621 gacacgccga acgagttgcc cgcccgtacg aagggtcacc gcgcccagga caaggagatc
     1681 gcccggctgg cgatccccgc gttcgccgcg ctgatctccg agccgctctt cctgctcgca
     1741 gacgccgcca tcgtcggtca cctcggcact gcccagctcg ctgctctcgg catcgccggg
     1801 acggtgatcc agacggcggt cggtctgttc gtcttcctcg cctacggcac cacatccacg
     1861 gttgctcgct ccgtcggcgc cggcgaccgg gcgggcgcgc tgagcgtggg cgtggacggg
     1921 atctggttgg cggtgatcat cgggacactc gccgcgaccg caaccgccct gctgacaccg
     1981 acgatcgttt ccggcttcgg cgcggccgac gacgtcgatg gctacacgac gacctaccta
     2041 cggatcgcag ccttcggcat accctccttg ttgctgctgc tggcggcaac cggtgttctg
     2101 cgaggactcc aggacacgcg cacgccgctc tacgtggcgg tggtggccaa cctcgccaac
     2161 atcgcgctca acgtcacgtt cgtgtacggg ttcgggtggg ggatcgccgg ctcagcagcg
     2221 ggcacgctcg tggcccagag cgtggccgct ctggccatcg tcgcggtcgt cgttcgcgcg
     2281 gcccggcggg tcggtgcgca gctggtgccg caccttccgg gggtcgcagc tgcgtggcgc
     2341 acgggagtac ccctgatcgt ccggacgttg tcgctccggt tggcgttgct gctcgcgacg
     2401 tacgtcgccg cggctatctc gacgccggcc gtggcggccc atcaggtcgc gttcacgatc
     2461 tggatgttcc tcgcgttcgc gctggacgcg atcgccatcg ccggacaggc catcaccggc
     2521 aagctgctcg gcgccggaga tgcggtcgct gcgcgggcag ccactcgccg gatgatgcag
     2581 tggggcctgg tgagcggcat cgccctcggc gtgggactgg ccctcatccg acccctgatc
     2641 gtgccgctgt tcacgtccga cgcgcaggtc caggacctgc tgtcgctcgt gttgctgatc
     2701 gccgccgcgc aacaaccggt gtcgggaatc gtgttcgtgc tcgacggtgt cctgatcggc
     2761 gccggggacg gccgattcct cgcctgggcc ggcgtcgtca acctcctggt gttcgtgccc
     2821 ctcgcacttg ccgtgccatg gctcggcggt gggatcggtt ggttgtgggg cgcgttcacg
     2881 gcgttcatgc tgatgcgtct ggtcacgctg ctgtggcgcg agcgcagcga cagctggctc
     2941 gtcctcggcg ccgcaccggc ggcgcgcaac cgctgaccgg cgtcgtggcc actgcgagtc
     3001 tccggcagtc gagctcgtcg aggcaccgca cctcgatctc gacgggcccg atcagctgga
     3061 gcggcgcgtc aggaagccgt cggtacgacg ttgagcttga ccgtggcgga cacctcgtcg
     3121 tgcaccttca cgatgacctc gtgggcaccg agggtcttga tcgggttgcc gacctggatc
     3181 cggcgcttgt cgatgtcggg accgcccgct gccctgacgg cgtcgacgat gtcggagagc
     3241 gtcacggcac cgaacagccg acccgacgag ccagcctggg cctgcacccg aacggtcagg
     3301 tcctcgagct gggccttgat ctcgtcggca tgcgagacgt cacggatgtc gcggatctcg
     3361 cgggccttct tgatcgagtc gatctgcttc tcggcaccac gggtccagcg gatggcagcg
     3421 ccacgaggga ggaggtagtt gcggccgtag ccgtccttga cgtcgaccac gtcaccgggg
     3481 gcgccgagat tggagacctc ttgggtcaag atgagcttca tcgcctggcc tcctctcagc
     3541 gcgcggtcga cgtgtagggc agcaacgcca tctcgcgggc gttcttgatc gccgtcgcga
     3601 cgtcgcgctg gtgctgggtg cagttaccgg tcacccggcg ggcgcggatc ttgccgcgat
     3661 cggagatgaa cttgcgcagc aacgtcgtgt ccttgtagtc gacgtacgtg gccttgtcct
     3721 tgcagaaggc acagaccttc ttcttgggct tgcggacaac gggcttggcc attgtggtgc
     3781 tctcctttca agagcccggc cgatcggccg gaatggtcag tgaggttcag gtggggctca
     3841 gaacggtggc tcgtccgacg acgacgcaga cggggaggcc caagggtcgt tggccggctg
     3901 gccggcgtgt gcgccggcag ccggtgccgg cgtggcccac gggtcactcc caccctggct
     3961 gacaccgccg gccgaggatc caccctccgc gccggagcgg ctcgcgcggg tgaccttcgc
     4021 cgtggcactg cgcaggctcg ggccgacttc gtcgacgtcg atctcgaaga cggtccgctt
     4081 ctcaccctcg cgggtctcga agctgcgcgc cttcagtcgg ccctgcacga tcacgcgcat
     4141 cccacgttgc agcgactcgg cgacgttctc ggctgcttgc cgccagacgg cgcaggtcat
     4201 gaacagcgtc tcgccgtcct tccactcgct cgtctgccgg tcgaaggtcc gcggcgtcga
     4261 cgcgatgcgg aagttcgcca ccgccgctcc cgaaggcgtg aagcggaggt cggggtcatc
     4321 gacgaggttg ccgacgatgg tgatgctggt ttcgcctgcc atggcagcct tccctgtgtg
     4381 tgtcaggtgt tcagttcgtg aggatcgatc ggaatgcccg acggcaaccg gtcgcgagcg
     4441 tcgtacgggc gtgacgtgga ggaaccgccg aggatcaatg cgcctctggt cggaggacct
     4501 tcgtccgcat cacggactcg ttgagaccga gctgccggtc gagctctttg acggtacccg
     4561 gctctgccga caacgtgacg acggcgtaga tgccctcgct cttcttgttg atctcgtagg
     4621 cgaggcgccg gcgaccccat acgtcgacgg actccacgct gccaccgtct tggcgaacga
     4681 ggttgaggta ttggtcgagg gacggctgga ccgtccgctc ctcgagttcg gggtcgagaa
     4741 tgaccatgag ctcgtagtga cgcatgagtg actccacctc ctgtggactc tgcggtcacg
     4801 gtctctccgt gacaggaggt cacgtcggac gcctgcgtgc ggcgactggt tgacgtggtg
     4861 cgacccggcg tcctgccggg tcagcgcagg caaggttatc agcgccacga ggaacgacgg
     4921 aaatccgcat ccacaggcct gttccgcaac ggcggcagtc gaatcggtgc gccgaccggc
     4981 tccgtccagg tgccagccga ccccgctccg gggcagatcc ggggactgct cagtcttgtg
     5041 tgggcggtga cggtcgcgat gggtggcgtg cttcccccgg cgcaccgtcc gcgtgcgctg
     5101 aggtgcgatg gcggcgatgg cgggagctgt gcgcgcgtca ggctgggctc ggccggcctt
     5161 ccgtctcttg tggtcggcct gtgagtggcg gtgtttcgga tgtccacggc tgaccggtgt
     5221 ggtgtttgcg atgttcacat cccagaccca ctgtgcccat cgaaggtttg cgatgtttac
     5281 agcccaaaag gcgtgtaaac atcgcaaacc ccgaggtacg gcctcagaaa gcggaccgcg
     5341 aaacgaaccc ctgagtgtga ggaccgcagg gttggtgggc cgcagggccg tccttcccgc
     5401 gaagaaggac gacccgcgac cacgtcggtg ggatcactcc gtggtgatcg cttcgaggac
     5461 atcgagcttg gccgcccgac gggccgggag caccgccgcc agcacaccga cgacggccga
     5521 gatcagcacg aagaccacca gctgcaccca tgggatcgac agcacgtcga caccctggtc
     5581 ggagatcgca cgctgcagcg caaccccgaa cccgatgccg aggacgacac cgagcacggc
     5641 gccgagtacg gcgatcgcga ccgactccaa ccggaccatg cgtcgcagct ggcgccgact
     5701 caggccaacc gcccggagca accccacctc ccgggtgcgc tcgatgaccg acagtgccag
     5761 cgtgttgatg atgccgagca ccgcgatcac gacggccagc ccgagcagcg cgtagatgat
     5821 cgccaggaac tggtcgatct gctgcttctg ctcgtcggcg tactcctgct ggttcttcag
     5881 cgtcaccgtc gggttgccgg ccaggatctc gtcgatcccg gcccggatgg aggcttcgct
     5941 ggccccgggt tcctggctga tgtagagcag cgagtcgcgg ggggcgtagc cgcctcgttg
     6001 catcgtgtcg agtgagacga ggtagtcggt gaagaccacg cctcccgacg ggtagattcc
     6061 cgcgaccttc aggttttccg tccgcgccgg gaacttcatc gcgacggtgt cgccgagcga
     6121 caggttctgc gacttcgcgg cgttggagct gacgaggacg gagtcgtcgg acagctccgt
     6181 gagtccaccg aactccatcg gtaggtcgag cgcctgcgcc agctctcgcg ggtcggtggc
     6241 cccgacgaag acctgctcgc cgtcgacctt cggtccggcc tcgcggaact gcgccacgct
     6301 tgccaccccg tccacctgcc ggatccgctc cgcgatcgac ggcgagaacg actgcgacac
     6361 ggcgttcgag acgatcagct gggcgttcag ggacgagtcg atcgccttgt ccgtgctcgc
     6421 cttcgccgac tgacccagga tggacatcag tgccaccagt gtcaggccga tcatgagcgc
     6481 gctcgccgtc gccgccgtac gccgggggtt gcgcagcgag ttctccttcg ccaacgtccc
     6541 gacggtgccg aagacgcggc ggtacagcgc tccgagaccc acgatgactg gccggccgac
     6601 cacgggcgag gtcagggcgg ccccgatcag gatgacgagc atgccgatcc cgatcagcag
     6661 cagcccacgg ctgccctcag cggtgaaccc gagcaccatc aacccgacgc cgaccaccat
     6721 catcgcgaca cccacgatga gccgccgacg caacgtggcc tgcggaagtg cgacgtcgtc
     6781 gcgcatcgcg gccaccggcg cgatccggcc ggcacgccgc gccggcaggt acgccgccag
     6841 catggtcacc acgaggccga cgaccagcga cacgacaatg gttcgcggct cgaccggcag
     6901 gctcgccgac ccgaggtcga gcccgaaggt ggcgaacagc gccttcagac cgatcgccag
     6961 cagatacccc actgcgatcc cgatgatcga tccgatcaga ccgaccacga atgcctcggc
     7021 cagcaccgac cggttgacct gacgcttcga ggcacccatg gcgcggagca gggcgagctc
     7081 acgactgcgc tgggcgacca ggatcgagaa cgtgttgatg atgaggaagg tgccgacgac
     7141 catcgagacg gccgcgaaga cgagcaggaa cgtgttgatg aagccgagcg ccttgccgat
     7201 cgcgtcctcg ttgtccttgg cgaccttgtc gccgggctcc gccgtcacac ctggcggcag
     7261 cgacggctga gcagcgtcgg cgagctgttc ttgagagacg ccgtctgccg cggtgagtga
     7321 gatcgacgtg acgacgtcgc ggccgtcgaa gaacaggtcc tgcagcgtgc gctcgtcgaa
     7381 gatcgtcagc gtcgccccga cgagtccccc ctcggagccg aacttcacca gtccggtgag
     7441 cgtcgccgtc atcgtcgggt tcctccccgg cgtgacgagc ttgacgtcgt ccccgatctg
     7501 gtatccggcc ttgtcagcgg tcccctcgtc gagcgcgatc tgggccggcc cctgcgggag
     7561 ctcccccttc tcgagcgtga ggatctggtc cccggtgatc gccgtcgtct cgttgaagtt
     7621 gaacgccagt ccgggcgggc cgttgccccc cacgagcttg cccttgtcgc tgatcacgta
     7681 gacaccgtcg acctggatca ccgggtccgc cgcggctgct tcggggagcg ccgccagctt
     7741 gggaacgacg gaagctggaa tcgtgcgggc gtcggagccg aagctcgtga agtcgttcgc
     7801 acccttgggc gcgacctcca cgtccgcggt cgtgcccttg atgatgccgt tgaacgcgtt
     7861 ccccaacgca tcggtgaaga tgaacgagcc ggcgacgaac gcgacaccga ggacgatggc
     7921 gaagccgctc aacgcgagcc ggatcttgcg cgccatcagg ttgcgccagg tgacc
//
LOCUS       DATBFZ010000323         8315 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_3500913, whole genome shotgun sequence.
ACCESSION   DATBFZ010000323 DATBFZ010000000
VERSION     DATBFZ010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 8315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8315
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3500913"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..655)
                     /locus_tag="VH419_10015"
     CDS             complement(<1..655)
                     /locus_tag="VH419_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020579103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY7043990.1"
                     /translation="MGDDGYDYDLLVVGSGPGGQRAAVAAAKLGKGVAVVDRRTMVGG
                     VCINTGTIPSKTMREAVIYLTGMNQRDMYGASYRVKDEITIADLTARTQHVIGREVEV
                     IRSQLLRNHIHLLTGTAHFTDPHTIVVEGVDRGDRATVTADKIVLAPGTQPARPAHVE
                     FDGRHVVDSDGILGLESIPSTLVVVGAGVIGIEYASMFAALGCRVTVVDQRPGMLEFC
                     "
     gene            717..1925
                     /locus_tag="VH419_10020"
     CDS             717..1925
                     /locus_tag="VH419_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009078416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HEY7043991.1"
                     /translation="MAAARTRTDRPYDVVLCGATGFTGGLTAERLAADGPEDLRWAIS
                     GRDGAKLEAVREWLAGLGKAGSGVEALRCDVEDAASVRALAEQTAVVATTVGPFLEYG
                     EPLVAACADAGTDYADIAGEPEFVDRMWLAHHERAERTGARVVHACGFDSVPHDLGVY
                     FTVQQLPQDVPITVSGYVRVGASPSAGTYHSAIGAFARVRQASEVAAKRRKLETRPAG
                     RRVRGLPRRPTRAPAGRGWALPLPTIDPVVVRRSARAVDRYGPDFRYGHFAVVRRLRT
                     AVVVPAALGGMVALAQIPPGRDLLLRRRQAGDDPSAARRARSWFKVRFEGAGGDRRVS
                     TEVSGGDPGYDETARMLAQSALCLAFDDLPDTAGQVTTVQAMGDALLRRLRDDGMRFE
                     VIESAGLPDE"
     gene            1918..2538
                     /locus_tag="VH419_10025"
     CDS             1918..2538
                     /locus_tag="VH419_10025"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY7043992.1"
                     /translation="MSDLFCPATVIVVRHAETEYDAPGIASDSGGSLSLTGREQARQL
                     GSSLASRRVAAIWCSDMARAVQTAEIAAAVLGVTVRVRPGLREFGVGGLAGQPITALD
                     EVFASWVGGDLAGGCPGAETGQDVVTRVSKELESIADQYRGETVLAVSHGGAMGVVLP
                     WLAGNVPADFAVGKPTDNCGTCELEADADGWVMKTWNGQQLAAYVD"
     gene            complement(2553..2924)
                     /locus_tag="VH419_10030"
     CDS             complement(2553..2924)
                     /locus_tag="VH419_10030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043993.1"
                     /translation="MPKTLYADGSVSHDVSGASCSVLLAPTGPPAKGPKALTKLPKVK
                     CCTSKPKCQRCPLRMLADGSLPDGYAVKKRRLIGPDGSPMSKAAMKKAGKELKRAKKA
                     GKAAKKAGKQAKKAGRLPEAA"
     gene            3055..3579
                     /locus_tag="VH419_10035"
     CDS             3055..3579
                     /locus_tag="VH419_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HEY7043994.1"
                     /translation="MASQKFVEQLNAQIGHEFSAHQQYVACAVYFDAKTMPRMAAFFY
                     RQALEERDHAMMMVQYLIDANADVEIPGAESPTNTFENIVAPVRLALDQENRVATQIN
                     DLTALARAENDYASDQFMQWFIKEQVEEVATMSDLLTVVIRAEHDVEAIEEYVARELG
                     DVGDDSTAPAVAGS"
     gene            3954..4793
                     /locus_tag="VH419_10040"
     CDS             3954..4793
                     /locus_tag="VH419_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017935529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7043995.1"
                     /translation="MEWIVIALIVLGVVVFMRSRRTPERTSLQARAPRQRVTAEDLDS
                     VRRAADEDVTQFGEELQRLDAELAGRELDEGMRQDYQRALDAYEDAKTSIAAVSHPDE
                     IRHVTEILGDGRYAVACVRARAAGEPLPARRPPCFFNPQHGPSVRDVGWAPDGGSERQ
                     VPACALDAERVEAGAEPASRQVMVGSRRVPYWQAGPAYAPFGAGYFGGFGLVEGLFVG
                     TLMGSAFGLFDGGGSADSGYDGGGGDGGDSFDAGGDMGMDFGGGDFGGGDFGGGDFGG
                     GDM"
     gene            4821..5582
                     /locus_tag="VH419_10045"
     CDS             4821..5582
                     /locus_tag="VH419_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014847645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEY7043996.1"
                     /translation="MAEDRTLVGVDVGGTGIKAALVDRTEGTLVTERVKVDTPRPATP
                     DNVAAVIAKLVGDIAPNDPSPVGVTVPGVVRSGVVHTAANIDKGWIGTDADQMLEHLL
                     HRPVHVMNDADAAGYAEVAFGSAKGHQGIVVITTLGTGIGSALVYHGVLVPNTELGHL
                     EIDGRDAELNASNAARERRKLSWEKWSKHLTKYYRTLEQLFSPDLFVVGGGVSKHAER
                     FLPLIEVDTTMVPAALGNSAGIIGAALLAYEDANH"
     gene            complement(5610..6284)
                     /gene="nucS"
                     /locus_tag="VH419_10050"
     CDS             complement(5610..6284)
                     /gene="nucS"
                     /locus_tag="VH419_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018550058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease NucS"
                     /protein_id="HEY7043997.1"
                     /translation="MRLVVARCQVDYAGRLTAHLPMATRLLIVKADGSVLVHSDGGSY
                     KPLNWMSPPCTLLEGVTDEGTVEWVVEAKSGDTLRIVIEETLSDTAYDLGLDPGLQKD
                     GVEKHLQELLAEHPHSLGAGLTLVRREFPTAIGPVDLLCRDPDGATIAVEIKRRGEID
                     GVEQLTRYLELLNRDPLLAPVSGIFAAQEIKPQARVLATDRGITCVTVDYDALRGMDD
                     PSARLF"
     gene            6471..8252
                     /locus_tag="VH419_10055"
     CDS             6471..8252
                     /locus_tag="VH419_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012922579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HEY7043998.1"
                     /translation="MSREFSTIGVVGLGTMGAGIAEVFARNGFSVVGVEQSDEQVERG
                     LAHVQHSTDRAKKRGKLGEEEQQAIFDRLTTTTSLADLKDCDLVVEAVLEHLDLKREI
                     FQRLDEIVRPDAVLATNTSSLSVTEISAATSNPGRVIGMHFFNPAPVQRFCEVIKTVV
                     TEPDVVEDVQALARKLGKNPVSVGDKAGFIANALLFGYLNHAVSMFESNYASREDIDA
                     AMRLGCGYPMGPLALLDLIGLDTAYEILDTMYRQGRNRLHAPSPILKQMTTAGLLGRK
                     TGRGFYTYEAPDSPIAVDDKQTPRPHDEPRFRREIQRVGVVGSGTMATGIVEVFAKAG
                     YDVTYVTRSSDKVENVRAGVTRSLEKAVQRGKLEESGRDAALRTMTGSTDIENLATVD
                     LVVEAIAEDLAVKKALFENLDEICRDGTILATTTSSLPVIELASVTSRPGDVIGMHFF
                     NPAQVMRLVEVVSTVATDDDVAETVRAVCQAIDKHPVSCGDRAGFIVNALLFPYLNDS
                     VRMLEAHYSTADDIDTAMKEGCALPLGPFELLDVVGNDVALAIERTLYLEFREPGFAP
                     APLLEHLVTAGYLGRKTGRGFRDYSSH"
BASE COUNT         1338 a         2715 c         2936 g         1326 t
ORIGIN      
        1 cgcagaactc gagcatcccc gggcgctggt cgacgacggt cactcggcag ccgagagcgg
       61 cgaacatcga cgcgtactcg atcccgatca caccggcgcc gaccacgacg agcgtcgacg
      121 ggatcgactc gagcccgagg atgccgtcgg agtcgacgac atgccggccg tcgaactcca
      181 cgtgagccgg acgggcaggc tgcgtcccgg gtgcgagcac gatcttgtcc gcggtgacgg
      241 tggcgcggtc gcctcggtcg accccttcga ccacgatcgt gtgcgggtcg gtgaaatggg
      301 cggtccccgt gaggaggtgg atgtggttgc gcagcaattg gctacggatg acctcgacct
      361 cacggccgat cacgtgctgg gtacgggcag tcaggtcggc gatcgtgatc tcgtccttca
      421 cccggtagct cgcgccgtac atgtcgcgct ggttcatccc ggtcaggtag atgacggcct
      481 cgcgcatcgt cttgctcggg atcgtgccgg tgttgatgca cacgccgccg accatcgtcc
      541 gccggtccac gacagcgact cccttgccga gcttggcagc cgcgaccgcg gcgcgctggc
      601 cgcctggacc gctcccgacg acgagcaggt cgtagtcgta gccatcgtcg cccatctgct
      661 catcgtgacg acggcaggcc ctgagcgcaa cgcagcggat cgggaatgat ggggacatgg
      721 ctgcggcgcg taccaggacg gaccggccgt acgacgtcgt gctgtgcggc gctaccggct
      781 tcaccggtgg gctgaccgcg gagcggctcg cggccgacgg tccggaagac ctgcgctggg
      841 ccatctcggg ccgcgacggg gccaagctgg aggcggttcg cgagtggctg gccggcctcg
      901 gcaaagccgg cagcggcgtc gaggcgctgc gctgtgacgt cgaggacgcc gcatcggtgc
      961 gtgcgctggc cgagcagact gctgtcgtgg cgacgacggt cggacccttc ctcgagtacg
     1021 gcgaaccgct ggtcgccgcc tgcgctgacg ctggcaccga ctacgccgac atcgctggcg
     1081 agccggagtt cgtcgaccgg atgtggcttg cacaccatga gcgagccgag cgcaccggcg
     1141 cccgggtcgt gcacgcctgc gggttcgact cggttccgca tgatctcggc gtctacttca
     1201 ccgtgcagca gctgccgcag gacgttccca tcaccgtgtc ggggtatgtg cgggtgggcg
     1261 cgagcccctc ggcaggcacc taccactcgg ctatcggcgc cttcgcccgc gtccgtcagg
     1321 cgtccgaggt cgctgcgaaa cgacgcaaac tcgagactcg gccggccggc cgacgggtac
     1381 gtgggcttcc ccgacggccg acgagggctc cggccggacg aggctgggcg ttgccgctgc
     1441 cgacgatcga ccccgtcgtc gtacgccggt cggcccgggc agtggaccgt tacgggccgg
     1501 acttccggta cggccacttc gcagtggtcc gcaggctgag gacagcggtg gtggtcccgg
     1561 ccgcgctggg cgggatggtc gccctcgcgc agatcccgcc gggccgcgac ctgttgctcc
     1621 ggaggcgaca ggccggcgat gacccctcgg ccgcccgccg cgcgaggtcg tggttcaagg
     1681 tgcggttcga aggtgccggc ggtgaccgga gggtgtcgac agaggtcagt ggcggcgatc
     1741 ccggctacga cgagaccgcc cggatgctgg ctcagtccgc tctctgcctt gcgttcgacg
     1801 accttcctga cacggccggg caggtgacaa ccgtgcaggc gatgggtgac gcgctgctgc
     1861 ggcggctgcg cgacgacggc atgcgcttcg aggtgatcga atcagcgggg ttgccggatg
     1921 agtgacctgt tctgtccggc gaccgtgatc gtggttcggc atgccgagac ggagtacgac
     1981 gccccgggca tcgcctccga cagtggtgga tcgctcagcc tgacggggcg ggagcaggcc
     2041 cgtcagctcg gctcgtcact cgcgagccgg cgggtggcgg cgatctggtg cagtgacatg
     2101 gcacgagccg tgcagacggc ggagatcgcg gctgccgtcc tcggtgtgac ggtgcgggtc
     2161 cggccgggac tgagggagtt cggcgtcggt gggctcgccg ggcagccgat cacggcgttg
     2221 gacgaagtgt tcgcgtcctg ggtcggggga gacctggccg gcggatgtcc gggtgccgaa
     2281 actggtcagg acgtcgtcac gcgggtgagc aaggagctcg agtcgatcgc cgaccagtac
     2341 cgcggtgaga ccgtgctcgc ggtgagtcac ggcggagcga tgggcgtggt cctgccctgg
     2401 ctggcgggca acgtgccggc ggacttcgcc gtgggcaaac cgaccgacaa ctgcgggacc
     2461 tgtgagctcg aggcagacgc cgacggctgg gtcatgaaga cctggaacgg tcagcagctc
     2521 gcggcctacg tcgactgagg tggccgtgac cgtcaggctg cctcggggag ccgacccgcc
     2581 ttcttggcct gcttgccggc cttcttggca gccttgcccg ccttcttggc cctcttgagc
     2641 tccttgccgg ccttcttcat cgccgccttg ctcatcgggc tgccgtccgg accgatcagc
     2701 cggcgtttct tcaccgcgta gccatccggc aggctgccgt cggcaagcat gcgcagcggg
     2761 cagcgttggc acttcggctt gctcgtgcag cacttgacct tgggaagttt cgtcagcgcc
     2821 ttgggcccct tggctggcgg tccggtgggg gcgaggagca cgctgcagga tgcgccgctc
     2881 acgtcgtgcg agacgctccc gtccgcgtac aaggtcttcg gcacggcgca gactctagca
     2941 tgcgatttag ggttgcctta cctatgctct ttctgcggcc gggcactcct gggatgatcg
     3001 gatcgtcggc tcgaccggcg tcgccctact cacatcggac cgaaggagac atccatggcc
     3061 tcgcagaagt tcgtcgagca gctcaacgcc cagatcggcc acgagttctc ggcacaccag
     3121 cagtacgtcg cctgcgctgt ctacttcgac gcgaagacca tgccgcggat ggctgcgttc
     3181 ttctaccggc aggcgctgga ggagcgcgac cacgcgatga tgatggtcca gtacctcatc
     3241 gacgcgaacg ccgacgtcga gatcccgggt gcggagtcgc ccacgaacac cttcgagaac
     3301 atcgtcgccc ccgtgcgcct ggcactcgac caagagaatc gcgtcgcgac tcagatcaac
     3361 gacctgaccg ccttggcccg tgccgagaac gactatgcct ccgaccagtt catgcagtgg
     3421 ttcatcaagg agcaggtcga ggaggtcgcg accatgtccg acctgttgac cgtcgtcatc
     3481 cgcgccgagc acgacgtcga ggcgatcgag gagtacgtcg cacgggagct cggcgacgtc
     3541 ggcgacgact cgaccgcccc cgcggtcgcc ggctcctagc gctggccagg gtactgaggc
     3601 tggtggtttg cgatgtttac agcccaaaaa gcgtgtaaac atcgcaaacc tcggaagggc
     3661 agaggcggtt tgccgtgtgg acatcgcaag ccgcaaagcc caggcccgtg cggatgaacc
     3721 ggtggtgggc gctgctcaac gccactccgg cgatggagcc tgtcgaagtt ccgtgacctc
     3781 gacctggacg cgatcgacca gcggagtacc gaggtggcac tcgacgtccg acacgccgac
     3841 gaaaagcaac gaaacctgcc acctcggcgg ttgaggggtc ggctgacagc ggtgcgtcgc
     3901 cacgcacgac gccggacgta ggctgaacga gtcagttagg agggaagcca gccatggagt
     3961 ggatcgtcat cgcgctgatc gtgctcgggg tggtggtgtt catgcgtagc cgtcgtacgc
     4021 cggagcgtac ctcgctccag gcccgggccc ctcggcagcg cgtcaccgcg gaggacctcg
     4081 acagcgtacg cagggcggcc gacgaggacg tgacgcagtt cggggaggaa ctccagcgcc
     4141 tggatgccga gcttgcgggc cgcgagctgg acgaagggat gcggcaggac taccagcggg
     4201 ccctcgacgc ctacgaggac gccaagacgt cgatcgccgc cgtcagtcat ccggacgaga
     4261 tcaggcacgt gaccgagatc ctcggcgacg gccgctacgc cgttgcctgc gtacgcgccc
     4321 gcgccgcggg cgaaccgttg ccggcgcggc gacccccctg cttcttcaac ccgcagcacg
     4381 gcccgtccgt gcgggacgtg ggctgggcgc cggacggcgg ttcggagcga caggtaccgg
     4441 cctgcgcgct cgatgctgaa cgggtcgaag cgggtgccga acctgcttcg cgtcaggtga
     4501 tggtgggctc gcgccgggtc ccgtactggc aggcgggacc ggcgtacgcg ccgttcgggg
     4561 ccggctactt cggcggtttc ggcctcgtcg agggtctgtt cgtcgggacg ctgatgggct
     4621 cggcgttcgg cttgttcgac ggcggcggct ccgccgactc gggctacgac ggtggcgggg
     4681 gtgacggtgg cgacagcttc gacgccggcg gcgacatggg catggacttc ggtggcggcg
     4741 acttcggcgg cggggacttc ggcggcgggg acttcggcgg cggcgacatg tgaacccgcg
     4801 ttggagggta tggctggacc atggctgaag accgaacgct cgtaggggtg gacgtcggtg
     4861 ggaccgggat caaggccgcg ctcgtcgacc gcacggaagg gacgctggtc accgagcggg
     4921 tcaaggtgga cacgccgcgc ccggccacgc cggacaacgt cgcggcagtc atcgcgaagc
     4981 tcgtcggcga catcgcgccg aacgacccgt caccggtcgg tgtcacggtt ccgggcgtgg
     5041 tcaggtcagg cgtggtgcac acggccgcga acatcgacaa gggctggatc ggcaccgacg
     5101 ccgaccagat gctggagcac ctcctgcacc gaccagtgca cgtgatgaac gacgccgacg
     5161 cggcgggcta cgccgaggtg gccttcggaa gtgccaaggg gcatcaaggc atcgtcgtga
     5221 tcacgacact cgggacgggg atcgggtcag ccctcgtcta ccacggtgtg ctcgtcccca
     5281 acaccgagct cggtcatctc gagatcgacg gccgtgacgc cgagctcaac gcgtccaacg
     5341 ccgcgcgcga acggcgaaag ctgagctggg agaagtggtc gaagcacctc acgaagtact
     5401 accgcacact ggaacagctc ttcagcccgg acctgttcgt cgtgggcggg ggcgtgagca
     5461 agcatgcgga acgcttcctg ccgttgatcg aggtcgatac cacgatggtg ccggccgcgc
     5521 tcgggaactc cgcaggcatc atcggcgcgg cgctgctcgc gtacgaggac gccaaccact
     5581 gagcagcggg ccagaatgga cacccggcgt cagaacagtc gagcggacgg atcgtccatg
     5641 ccgcgcaacg cgtcgtagtc gaccgtgaca caggtgatgc cgcggtcggt ggcgaggacc
     5701 cgcgcctgcg gcttgatctc ctgggcggcg aagatgccgg agacgggggc cagcagcggg
     5761 tcgcggttga gcagttcgag gtaacgggtc agctgctcga ctccgtcgat ctcaccgcga
     5821 cgcttgatct ccacggcgat cgtggcaccg tcggggtcgc ggcagagcaa gtcgacgggg
     5881 ccgatggcag tggggaactc ccgccgtaca agtgtcagcc cggctcccag cgagtgcgga
     5941 tgctcggcga gcagctcctg gaggtgtttc tcgacgccgt ccttctgcag accgggatcg
     6001 aggcccaggt cgtacgccgt gtcgctgagc gtctcttcga tcacgatgcg cagcgtgtcg
     6061 ccggacttcg cctcgaccac ccactcgacc gtgccctcgt cggtcacgcc ctcgagcaac
     6121 gtgcacggcg gagacatcca gttcagcggc ttgtacgacc cgccgtcgga gtgcacgagc
     6181 acggatccat cggctttgac gatcagcaac cgggtggcca tcggtagatg cgcggtgagc
     6241 cgaccggcgt agtcgacctg gcacctggcc accacgagac gcacgggtct gaccctagcg
     6301 agtcatcggt tgagctgagg gcgtcggcgg cttgcgaact ggtcccgagg tggcggcttt
     6361 cgtccagggc acgcgaaggg tggacgaaaa ccgccacctc ggttcctgat gaggccaatc
     6421 gacagtgacc gtagtctggg ctcggtctgg tatctgggag actcgtgggc atgtctcgag
     6481 agttctcaac gatcggtgtc gtcggtctgg gcaccatggg tgccggtatc gccgaggtgt
     6541 tcgcgcgcaa cgggttctcc gtggtgggcg tcgagcagag cgacgagcag gtcgaacgtg
     6601 gtctggccca cgtccagcac tcgaccgacc gcgccaagaa gcgggggaag ctcggcgagg
     6661 aggagcagca ggcaatcttc gaccggctca ccacgaccac gtcgctggcc gacctcaagg
     6721 actgcgacct ggtcgtcgag gcggtcctcg agcacctcga cctgaagcga gagatcttcc
     6781 agcggctcga cgagatcgtc cggccggacg cggtcctcgc cacgaacacg tccagcctgt
     6841 cggtcaccga gatctctgcg gcaacgtcca accccggccg ggtgatcggg atgcacttct
     6901 tcaaccctgc gccggtgcag cggttctgcg aggtcatcaa gacagtcgtc accgagccgg
     6961 atgtcgtgga ggatgtgcag gctctcgcgc gcaagctcgg caagaaccct gtctcggtgg
     7021 gcgacaaggc gggtttcatc gccaacgcgc tgctcttcgg ctatctcaac cacgctgtct
     7081 ccatgttcga gagcaactat gcgagccgcg aggacatcga cgccgcgatg cgcctgggct
     7141 gcggttaccc gatgggcccg ctcgcactgc tcgacctgat cgggctcgac acggcgtacg
     7201 agatcctcga cacgatgtac aggcagggcc gcaaccggct gcacgcgccg agcccgatcc
     7261 tgaagcagat gaccaccgcc ggactgctcg gccgcaagac cggtcgcggc ttctacacgt
     7321 acgaggcgcc ggacagcccc atcgcggtcg acgacaaaca gacgccgagg ccgcacgacg
     7381 agcctcgctt ccggcgggag atccagcggg tgggggtcgt cggttcggga acgatggcga
     7441 ccgggatcgt cgaggtgttc gccaaggcgg ggtacgacgt cacgtacgtc acgcgaagca
     7501 gcgacaaggt cgagaacgtc cgtgccggcg tcacacgcag tctcgagaag gcggtccaac
     7561 gcggcaagct cgaggagtcc ggccgcgacg cggcgttgcg gacgatgacc ggctcgacgg
     7621 acatcgagaa cctggccacc gtcgacctcg tcgtcgaggc gatcgccgag gaccttgccg
     7681 tcaagaaggc gctgttcgag aacctcgacg agatctgccg cgacggcacc atcctcgcca
     7741 ccacgacgtc gtccctgccg gtcatcgagc tggcatccgt caccagtcgg cccggcgacg
     7801 tcatcggcat gcacttcttc aatcccgcac aggtgatgag gctggtcgag gtcgtctcga
     7861 cggtggccac cgacgacgac gtcgccgaaa ccgttcgggc cgtgtgccag gcgatcgaca
     7921 agcatccggt gtcgtgcggc gaccgcgccg gcttcatcgt caacgcgttg ctgttcccct
     7981 acctcaacga ctcggtccgc atgctcgagg cgcactattc gacggccgac gacatcgaca
     8041 ccgcgatgaa ggaaggatgc gccctcccgt tgggaccgtt cgagctgctc gacgtggtcg
     8101 gcaacgacgt cgcgctggcg atcgagcgca cgctctacct ggagttccgt gagcctggct
     8161 tcgcgcccgc gccgctgttg gagcacctcg tcacggcggg ctacctgggt cgcaagacgg
     8221 gtcgtggctt ccgcgactac tcgtcccact gacgggcgag atcggcatac gacgtgtcgc
     8281 atcgcaccgg tcgaggccac cggatccggt ccaac
//
LOCUS       DATBFZ010000324         3134 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_551756, whole genome shotgun sequence.
ACCESSION   DATBFZ010000324 DATBFZ010000000
VERSION     DATBFZ010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 3134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3134
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_551756"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..789
                     /locus_tag="VH419_10060"
     CDS             <1..789
                     /locus_tag="VH419_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEY7043999.1"
                     /translation="LGAELGARIYVFWGGREGVEAMAAKSPIDALDRYREAIDYLCEY
                     VRERGYDLRFALEPKPNEPRGDLFLPTVGHALAFIGGLAHPEMVGLNPEVAHETMAGL
                     SFFHGVAQALWAGKLFHIDLNGQKIGRYDQDLRFGSEEVKDAFFLVRLLEHAGYDGPK
                     HFDARPYRVEDDEGVWDFAAGCMRTYLALAQKARAFDADPEIQEALADAGAFALGRPT
                     VGTYSRASAEALVGEVFDVEALAARGYRNDRLDQLTVELLLGLR"
     gene            855..2237
                     /locus_tag="VH419_10065"
     CDS             855..2237
                     /locus_tag="VH419_10065"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY7044000.1"
                     /translation="MHVKSRVLGVMAGLALVGAACGSSGGGGSGASGSAAPAATSGAS
                     GTTAPAAPATSAAPATSSAKPVTINWWHIQNNDPGLSLWKAVAAEYTQAHPNVKINIT
                     VLENEAFKAKLTTALQAGDVPDLFQSWGGGGLEEQVKAGLVKDITADVAPWINDLNKG
                     AVGLYQVDGKQYGIPFDLGMVGFWYNKELFQKAGISAPPTTWDEFLSDVGKLKAAGIT
                     PISLGEGDKWPGMFWWAYLSLRLGGESAMTQAGKDGSFTSQPFIDAGTQLKKLVDMQP
                     FQKGYLAAAWDGAGGEAATIATGGAAMHLMGQWAPGTQNANSPDGKGLGDKLGWFPFP
                     SVNGGKGLATDAFGGGNGFAVGKNAPPEAVDFLKYATSLDVAKRWGATNSGILPVTVG
                     ADSSITDKDLQTVLENRAKATYVQLYLDQAYAPAVGAAVNDAVQQLFAGKATPDQVAK
                     TIANAAKTGQ"
     gene            2215..>3134
                     /locus_tag="VH419_10070"
     CDS             2215..>3134
                     /locus_tag="VH419_10070"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEY7044001.1"
                     /translation="MPQRQVSSTTEPRERRVVIDGPPLGHNGQHAPVTRVRRTDRRPS
                     GVRMRRKPLVIALFLLPALCLYLLLVILPIIQGIHYSLYHWNGLEPLTNFVGLQNYRT
                     ALADPVFTGALKHNVVIVVLSLVIQLPFALGLAVLLNQRIHARALLRLLFFLPFVLSE
                     VITGVIWRLLLKPDGLVDKSFHVAGLGSLVHEWLADPSIVLYTLFFVISWKYFGFHMV
                     LFLAGLQQIPHELHEAAAIDGATQLQVFRHVTLPLLGPTIRISVFLSIIGSLQLFDLV
                     WVMTGGGPVNASNTMAVYMIDHSFKRFQFG"
BASE COUNT          519 a         1047 c         1046 g          522 t
ORIGIN      
        1 ctcggcgccg agctcggggc gcgcatctac gtgttctggg gcggacgaga aggcgtggag
       61 gcgatggcgg ccaagtcgcc gatcgacgcc ctcgaccgct accgcgaggc catcgactac
      121 ttgtgcgagt acgtgcgcga gcggggctat gacctgcggt tcgcgctgga accgaagccg
      181 aacgaaccgc gcggcgatct gttcctgccc accgtcggcc acgcgctggc gttcatcggg
      241 ggcctcgccc acccggagat ggtgggtctc aaccccgagg tcgcccacga gaccatggcc
      301 ggtctctcct tcttccacgg ggtcgcgcag gcgctgtggg cgggaaagct gttccacatc
      361 gacctgaacg gccagaagat cggccgttac gaccaggatc tgcgattcgg gtccgaggag
      421 gtcaaggacg ccttcttcct ggttcgactg ctcgagcacg ctggctacga cggcccgaag
      481 cacttcgatg ccaggccgta ccgcgtcgag gacgatgagg gggtctggga cttcgccgct
      541 ggttgcatgc gcacctatct cgcgctcgcg cagaaggcgc gggccttcga cgccgatccc
      601 gagatccaag aggcgctggc tgacgcgggc gcgttcgcgc ttggtcgccc gacggtcggc
      661 acctacagcc gggcgagcgc cgaagcgctg gttggggagg tgttcgacgt cgaggcgttg
      721 gccgctcgtg ggtatcgcaa cgaccggctc gatcagctca ccgtggagct gttgctcggt
      781 ctgcgctgaa gaggtctgac gacaggctgc gggcccctgt gcccgtgggg ggagatgaga
      841 aaggagtcag agcaatgcat gtgaaatctc gggtcctggg ggtgatggct ggcctcgcct
      901 tggtcggtgc cgcctgcggg agcagcggtg gtgggggatc gggggcctcg gggtccgcgg
      961 cacctgccgc cacgtccggt gcgagcggga ccactgctcc ggcagcaccc gcgacctccg
     1021 ccgcaccagc gacgagcagt gcaaagccgg tcaccatcaa ctggtggcac atccagaaca
     1081 acgatccggg tctgtccttg tggaaggcgg tggccgccga atacacgcag gcccacccga
     1141 acgtgaagat caacatcacc gtgctcgaga acgaggcctt caaggccaag ctgacgaccg
     1201 cgctccaagc cggtgacgtg cccgatctct tccagtcctg gggcggcggt ggtctcgagg
     1261 aacaggtcaa ggcggggttg gtgaaggaca tcaccgccga cgtggcaccg tggatcaacg
     1321 acctcaacaa gggcgcggtc ggtctctatc aggtcgacgg caagcagtac ggcatcccgt
     1381 tcgacctcgg catggtcggg ttctggtaca acaaggagct cttccagaag gccgggatca
     1441 gcgcaccgcc aaccacgtgg gacgagttcc tgtccgacgt cggcaagctg aaggccgcgg
     1501 gcatcacgcc gatctcgctc ggtgagggcg acaagtggcc agggatgttc tggtgggcgt
     1561 acctgtcgtt gcgcctcggc ggcgagagcg ccatgaccca ggccggcaag gacggttcgt
     1621 tcacctcgca accgttcatc gacgccggca cacagctcaa gaagctggtc gacatgcagc
     1681 cgttccagaa gggctacctc gcggcggcgt gggacggcgc cggcggcgag gcggccacca
     1741 tcgccacggg cggtgcggcc atgcacctga tgggccagtg ggcgccgggg acgcagaacg
     1801 ccaactcgcc cgacggcaag ggtctcggcg acaagctcgg ctggttcccg ttcccgagcg
     1861 tcaatggcgg caagggtctg gccaccgacg ccttcggcgg cggcaacggc ttcgccgtcg
     1921 gaaagaacgc gccacctgaa gccgtggact tcctgaagta cgcgacgagc ctcgacgtcg
     1981 ccaagcggtg gggtgcgacg aacagtggca tcctgccggt gaccgtcggt gccgacagct
     2041 cgatcacgga caaggatctc cagaccgtcc tggagaaccg ggcgaaggcc acgtacgtgc
     2101 agctctacct ggaccaggcg tacgcccctg cagtgggtgc cgccgtgaac gacgccgtcc
     2161 agcagctgtt cgccgggaag gcgacaccgg accaggtcgc caagaccatc gccaatgccg
     2221 caaagacagg tcagtagcac caccgaaccc cgcgagcggc gggtcgtcat cgacggcccg
     2281 ccgctcggcc acaacgggca gcacgccccg gtgacccggg ttcgccggac ggaccgccgc
     2341 ccgtccgggg tccgaatgcg ccggaagccg ctcgtcatcg cgctcttcct cctgccggcg
     2401 ctctgcctct acctgctgct cgtgatcctc ccgatcatcc aagggatcca ctacagcctg
     2461 taccactgga acgggctcga gccgctcacg aacttcgtcg ggctccagaa ctaccgcacg
     2521 gctctcgctg acccggtctt cacgggcgcg ctgaagcaca acgtcgtcat cgtcgtgctg
     2581 tcgctcgtga tccaactccc gttcgcgctc ggcctcgccg tgctcctcaa ccagcggatc
     2641 cacgcgcggg cgttgctccg cctgctcttc ttcctgccgt tcgtgctgtc cgaggtgatc
     2701 accggggtga tctggcggct cctgctcaag ccggacggtc tggtcgacaa gtcgttccac
     2761 gtcgccggac tgggttcgct cgtgcacgaa tggctggccg atccgagcat cgtgctgtac
     2821 acgctgttct tcgtgatctc gtggaagtac ttcggcttcc acatggtgct ctttctcgcc
     2881 gggctgcagc agatccccca cgagctccac gaggcggcgg ccatcgacgg cgccacccag
     2941 ctccaggtgt tccgccacgt caccctgccg ctgctcggac cgacgatcag gatctccgtg
     3001 ttcctgtcga tcatcggctc gctgcagctc ttcgatctcg tgtgggtgat gaccggcggt
     3061 ggtccggtca acgcgtcgaa cacgatggcc gtttacatga tcgaccacag cttcaagcgc
     3121 ttccagttcg gcta
//
LOCUS       DATBFZ010000325         4003 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_4379297, whole genome shotgun sequence.
ACCESSION   DATBFZ010000325 DATBFZ010000000
VERSION     DATBFZ010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 4003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4003
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4379297"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="VH419_10075"
     CDS             <1..546
                     /locus_tag="VH419_10075"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY7044002.1"
                     /translation="VESEWGPVDVLVANAGGGVSARVIRTTDDDWQRMLDLNLTAPFR
                     CVRRALPAMLAADHGRVVVIASVAAKVGEPYISAYTASKHGVLGLVRSAAAEVARTGV
                     TVNAICPGYVDTPMTDSTVAGIVTMTGRSEEEARRIIADKQPTGRLVTPEEVAKAVWL
                     CVVNGSINGQGINVDGGAIQS"
     gene            543..935
                     /locus_tag="VH419_10080"
     CDS             543..935
                     /locus_tag="VH419_10080"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009083722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEY7044003.1"
                     /translation="MSLERVNPGSLTKPRGFSHAVVAEGRMVFLAGQTGLDVDGHVVA
                     GGVVAQFEQALSNLLTALEEAGGRPDQLTSMTIYIVDMVGYREHATEIGRVWRNLVGS
                     EYPAMAGIGVARLWDDDALVEVQGFAVL"
     gene            complement(1239..2066)
                     /locus_tag="VH419_10085"
     CDS             complement(1239..2066)
                     /locus_tag="VH419_10085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044004.1"
                     /translation="MNTTLKRLVVAARAVADVEPARIEATVLQFSGTRRWLAPVAWAA
                     GTLVLVVRGVKLLILNWRLTMIELIPAVWVWVAMWDLKQHRLRAEPLRQLTPGQALLG
                     VGVAIAFSVAAFWFNTVFGFAITHQPPRIGLAWQQARPHLRRVVTLGTGMGIVVAAGL
                     AWIPRIDSGWLFLAAAFSLYGLMLVALVVVPARILGVIKQRMTPKQTIGRWAAGGALS
                     AVAMTPGFVLDRVGIIMLGIPGFHLLGLVVLSIGTALYAAGLSSVKAIKLSMKLETR"
     gene            2233..3660
                     /locus_tag="VH419_10090"
     CDS             2233..3660
                     /locus_tag="VH419_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 domain-containing protein"
                     /protein_id="HEY7044005.1"
                     /translation="MTAELSSARTRTPEARAAWGKAARERAPLEAHADFRPAERRDPV
                     GLLLSQVDTRVPELVPIRHGRMLVSPFTFYRGAALVMAADLSATQASGLQTQLCGDAH
                     LSNFGAYASPERRLVFDINDFDETLPGPFEWDVKRLAVSFVVAGRDNGFSVKQCRKAT
                     LAGVEGYRRAMRDFAKQPIVSVWYAHLEIEEAIAEIKSSLSDHQREKRREQIEATEAL
                     LAKARTRDSLHAIAKLTTVVDGRRRIISDPPLVVPIDELAGLDSEVVFQRLQQLLSSY
                     RDTLQADRRHLFDHFTLADVAHKVVGVGSVGTRAWIVLLEGGTPAEALLLQAKQAERS
                     VLADYTGGSEYANQGERVVTGQRLMQATSDIFLGWLRALPVDGRDQDYYVRQLRDWKV
                     SAAIEDMNPQGMKVYAQLCGWTLARAHARAGDRIAIAAYLGKSGTFDEAVADFAEAYA
                     DQNERDHATLSAAVVDGRVEARSDV"
     gene            complement(3792..>4003)
                     /locus_tag="VH419_10095"
     CDS             complement(3792..>4003)
                     /locus_tag="VH419_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009083718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PaaX family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HEY7044006.1"
                     /translation="ATSGTSADADAFADYVSTLTAWRRLPYLDPGLPDDLLPKGWSGA
                     RAADLFFDLRDRLSRPAADFVTTVR"
BASE COUNT          668 a         1294 c         1427 g          614 t
ORIGIN      
        1 gtcgaatcgg agtgggggcc ggtcgacgtc ctcgtcgcga acgccggtgg gggtgtttcc
       61 gcccgggtga tcaggaccac cgacgacgac tggcagcgga tgctcgacct caacctgacg
      121 gcgccgtttc gctgtgtccg ccgcgcgctg ccggccatgc tcgccgccga ccacggccgc
      181 gtcgtcgtga tcgcgagtgt ggcggccaag gtcggcgagc cgtacatctc ggcgtacacc
      241 gcgagcaagc acggagtgct cggtctcgtc cggtcggcgg cggcggaggt cgcgcgtacc
      301 ggtgtcaccg tcaacgcgat ctgtcccggg tacgtcgaca cgccgatgac ggacagtacg
      361 gtggccggca tcgtgacgat gacggggcgc agcgaggagg aagcacgacg gatcatcgct
      421 gacaagcagc cgacgggacg actcgtcacg ccagaggagg tggcgaaggc cgtgtggctg
      481 tgtgtcgtca acggttcgat caacgggcaa gggatcaatg tcgacggggg cgcgatccag
      541 tcatgagtct cgagcgggtc aaccctgggt cgctgacgaa accccgtggg ttctctcacg
      601 cggtcgttgc tgagggtcgc atggtgttcc tggccggcca gaccgggctc gacgtcgacg
      661 ggcacgtggt cgcaggtggc gttgtcgcgc agttcgagca ggcgctcagc aacctgctca
      721 ccgcgctcga ggaagccggc ggccgcccgg accagctcac ctcgatgacg atctacatcg
      781 tggacatggt cggctaccgg gagcacgcca ccgagatcgg tcgcgtctgg cgaaacctcg
      841 tcgggagcga gtacccggcg atggccggca tcggcgtcgc tcgcctctgg gacgacgacg
      901 ccctggtcga ggtccaaggg ttcgccgtgc tttgagccgt cacgcgtctg tcgcagctgg
      961 ttgcccggcg gcggtttgca tggctaacca gccaattcca tccctcccga ggcagacctg
     1021 cctggctacc cgtccgtttg gatggctagc gaggcaaacc accaccgacg tacgagcctt
     1081 ctggctcttc gtcggtgact gagccaccat ggacgtgtct ggggcgagcg ttcgcctggc
     1141 cgaggacaga ccgtcggcgc ctgaggtgtt gccggaggaa gcacccttga cagacatctc
     1201 ggccggaccc gaccgctgac gcctcgggac gggtggcctc agcgagtctc gagtttcatg
     1261 ctgagcttga tggccttcac cgaggagagc ccggcggcgt acaacgcggt cccgatcgac
     1321 agcacgacaa gaccgagcag gtggaagccc gggatgccga gcatgatgat gccgacccgg
     1381 tcgagcacga accccggcgt catggccacc gcgctgagcg caccaccggc cgcccatcgt
     1441 ccgatggtct gcttgggcgt catccgttgc ttgatgacgc cgaggatgcg agccggtacg
     1501 acgaccagcg ccaccagcat gaggccgtac agactgaagg cggcggcgag gaacagccag
     1561 cctgagtcga tcctcgggat ccaggccagc cctgcggcga cgacgatccc catccccgtg
     1621 ccgagcgtca cgacgcggcg cagatgcggc ctggcctgtt gccaggcgag gccgatccgg
     1681 ggtggctgat gcgtgatcgc gaaaccgaag accgtgttga accagaaggc ggcgacgctg
     1741 aaggcgatgg cgacgccgac gcccaggagc gcctgaccgg gagtgagctg ccggagcggc
     1801 tcggcacgca gccggtgttg cttgaggtcc cacatcgcga cccacaccca caccgccggg
     1861 atgagctcga tcatcgtgag tcgccagttc aggatgagga gcttgacccc gcggacgacc
     1921 agcaccaatg tgccggccgc ccacgccacg ggggcgagcc agcgacgagt gccgctgaac
     1981 tgcagcaccg tcgcctcgat gcgggcgggt tcgacgtcgg ccactgcgcg agcggcgacg
     2041 accaatcgct tcaaggtggt gttcatgtgc cgcctcgccg acggtgggtc tcgcacccta
     2101 gcgtgaccct tcgactcgac gcgctcaatg ccggcaccgg ctgtgtcgcc cacgagtcgg
     2161 gcggcgtcgt actgatctgg ggctgcgatg ataacggtga aaccggctcc tgccggttcg
     2221 aaggaggccg tcatgacagc ggagctgtca tctgcccgta cccggactcc cgaggcgcgg
     2281 gcggcatggg ggaaggccgc ccgagagcgc gcgccattgg aagcgcacgc tgacttccgg
     2341 ccggccgaac gacgtgaccc ggtcggactg ctgctcagcc aggtggacac gcgcgtgccg
     2401 gagctggtgc cgatccggca cggccggatg ctggtgtcgc cgttcacgtt ctaccgtggc
     2461 gcggcgctgg tgatggccgc ggatctgtcc gccacgcagg catcaggtct gcaaacacag
     2521 ctgtgcggag acgcgcacct gtccaacttc ggtgcctatg cctcgcctga gcgtcgactg
     2581 gtgttcgaca tcaacgactt cgacgagaca ctgcctgggc ccttcgagtg ggacgtcaag
     2641 cgcctggcgg tgagcttcgt cgtggcgggc cgtgacaacg ggttcagcgt caagcagtgc
     2701 cgcaaggcca cgctggcggg cgtggagggc taccgccgag cgatgcgcga cttcgcgaag
     2761 cagccgatcg tctcggtctg gtacgcgcat ctggagatcg aggaggcgat cgccgagatc
     2821 aagtcgtcgt tgagcgatca ccagcgcgag aagcggaggg aacagatcga ggccacggag
     2881 gcgttgctgg cgaaggcgcg gacgcgtgac agcctgcacg cgatcgccaa gctgacgacg
     2941 gtggtcgacg gacggcgacg gatcatcagc gacccgccgc tggtcgtccc gatcgacgag
     3001 ctggcgggcc tcgacagtga ggtcgtcttc caacgcctgc aacagttgct gtcgtcgtac
     3061 cgcgacacgc tccaagccga ccgccggcac ctcttcgacc acttcactct tgccgacgtc
     3121 gcgcacaagg tcgtcggtgt cggcagcgtc gggacacgcg cgtggatcgt cctgctcgaa
     3181 gggggcaccc ccgcggaagc ccttctcctg caggcgaaac aggccgagcg ctcggtcctg
     3241 gcggactaca ccggcgggtc ggagtacgcc aaccaggggg agcgcgtggt caccggacag
     3301 cggctgatgc aggcgacgag cgacatcttc ctcggctggc tgcgcgcgct gccggtcgat
     3361 gggcgggacc aggactacta cgtccgtcag ctgcgggact ggaaggtgtc cgccgccatc
     3421 gaggacatga acccgcaagg catgaaggtg tacgcccagc tctgtggctg gacgctggcg
     3481 cgtgctcacg cgcgagccgg cgaccggatc gcgattgcgg cgtacctcgg caagtcgggc
     3541 acgttcgacg aggcagtggc cgacttcgca gaggcctacg ccgaccagaa cgagcgcgac
     3601 cacgccacgc tgtcggctgc ggtcgtcgat ggtcgcgtcg aggcacgatc cgacgtctga
     3661 acggcctgcg gcgtcgagct tgtcgagccg tcacccggtg atctcgacgg gctccatcag
     3721 cgggccgggg gatccggcgg tcgagcttgt cgagaccccg gaagactcgg gtagcttccc
     3781 ggcccatcgt ctcagcgaac ggttgtgacg aagtcggccg cgggacgtga caatcgatcc
     3841 cggaggtcga agaagaggtc tgctgcacgg gcgccggacc agcccttggg gaggaggtcg
     3901 tcggggagtc ccgggtccag gtagggcagc cggcgccagg ctgtgagcgt gctgacgtaa
     3961 tcggcgaacg cgtcggcgtc ggccgaagtc cccgaggttg cgc
//
LOCUS       DATBFZ010000326         7890 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_552405, whole genome shotgun sequence.
ACCESSION   DATBFZ010000326 DATBFZ010000000
VERSION     DATBFZ010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 7890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7890
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_552405"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="VH419_10100"
     CDS             complement(<1..187)
                     /locus_tag="VH419_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016455351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate hydrolase family protein"
                     /protein_id="HEY7044007.1"
                     /translation="MRAQVLERFRAQLDDGGVLVGAGAGTGLSAKCAEAGGADLIIIY
                     NSGRYRMAGRGSLAGLMP"
     gene            complement(184..1401)
                     /locus_tag="VH419_10105"
     CDS             complement(184..1401)
                     /locus_tag="VH419_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020574563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tm-1-like ATP-binding domain-containing protein"
                     /protein_id="HEY7044008.1"
                     /translation="MPNVVVIGTLDTKGAEYAFLCERIRAHGADVVLVDAGIIGEPSV
                     TADISRNDVAGAVGADVAALAAAGDRGAAIEAMSRGAAAVATRLLNEGRLDAVVGMGG
                     TGGTSLVSYAMQQLPVGVPKLVVSTVASGETRPYVGAVDITMMYSVVDVAGVNQISAR
                     IIANAAAAVAGMAGAEAPSLGDMRPLIGASMFGVTTPCVTVGRERLESLSYEVLVFHQ
                     TGTGGQSMEELVRAGFIKGVLDVTTTELCDELVGGVLRAHAKRLEVAGSLGVPQVVSL
                     GALDMVNFGPMDTVPAQFRSRTLYVHNPTVTLMRTTPDECAELGRRVAQKLNTATGPT
                     ALFVPLRGVSMIATEGQPFYDSKADDALFSSIRETVGPNVEVHEVDTDVNDPDFALAM
                     ANRLHAMIGEARS"
     gene            complement(1436..3499)
                     /locus_tag="VH419_10110"
     CDS             complement(1436..3499)
                     /locus_tag="VH419_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012922202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044009.1"
                     /translation="MERGVYFDAWFPRQHCYHPSLPPRRLRMIDDLEAYHATSLVWSA
                     LGGGSISLPYLEQEAWGEVDERFRFYGFVNDAEFIKHCQDRGIKVFGCVFEVQGWEFP
                     VELDDDESTVLSMNEVRGVGKRGWLGLREFTQNRYPKIWKALEEYFPDGLVNSDGETV
                     TDILEECTSRDIHGNACHSTWVEVPGNDHYAYLMDRNNPVWREYLKAIIRIQIDAGVA
                     GVHLDEADLPIFATGYGGCFCKDCMKLFTAYLQELSSDRLPEPLKDTDLDTFHYGSWL
                     LERGYDFKSDREATPLYWDYIRFQRKTIVGYFAELADYIKEYGRSKGRDVLVCGNYYY
                     LSPHYYPFEPKADILVTEMNATSYRQPNWCRYAAGFARGKPVIVVENPYGGVGPELLP
                     KLQAGKGYDLYRMMQYEASALGINMSVPYGAWMGSVIEDSFWAPHELNVEIQDFIATN
                     ERLYEPETYSEAVVLYSVQSAYDWEEHKGWKVKFPFWTATEALVEQHQPFDVIVLPEG
                     ELREDWVTAGDLARYRTIVLPECTFLTPIQAKALLGYLERGGMVLATGDLGANLDPGE
                     RSALLAHPHLTRTADVRSEYFTGGPQAIVEPGVDLALNVTRVGDKEAAVHIIRYDYDE
                     ERDEVPVLDKLSLDIRISRPFRTVSALSPTGEVPARLTFSRERREMHRVELENVPLYT
                     VVLLQ"
     gene            complement(3534..4541)
                     /locus_tag="VH419_10115"
     CDS             complement(3534..4541)
                     /locus_tag="VH419_10115"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HEY7044010.1"
                     /translation="MRAPAIVFAEPKRIEIKEMDLPEVGPGQVGIRTAFSGVSQGTER
                     WALTGRYGHYDHDYSAYYPCSPGYQAAGVVDVVGSEVDSLATGDHVFTMGTHFADPVH
                     KYPGPCQASHSAYLVANAAEVWRVDPGVDLAGASLFHMAGVSRHGVRLTGVEPDDLVV
                     VIGLGMIGQMSAQAARHAGARVVATDLIRLRVEAAARHSADRGVDPEAESLEEVVRTE
                     HPDGADVVIDTTGVARMFDRCLDLVKYEGKIVMQGYYPDPISIDFHPTHLKRPHVVFP
                     CGWDDEFNDDLADDMAKGSIAIDPLITHRMPYRDAHQVYELVVDHPEQSLGMVLDWSG
                     A"
     gene            complement(4609..7305)
                     /locus_tag="VH419_10120"
     CDS             complement(4609..7305)
                     /locus_tag="VH419_10120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044011.1"
                     /translation="MISFPHFLNTSPRISLACGRDDRLAGLTPVDWATRRLQAALAAK
                     GAMADRTEQPDPVADLAILIGGADHGRSQEILSRLGLAVPTVPESLALVPFESEGQRR
                     LLAAGHDERGLVYAILELADRVEHAADPIEAMRFDTATIEQPANAVRSVARCFASEVD
                     DKRWLQDEAFWHDYLTMAVTQRFNRVNLMVGLGYNFPWHVTDAYLFFPYPFLLDVPGY
                     RVRIPQLPDEERDRNLAMLQFASEQAVVRGLDFQVGLWTHAYEWFDSPHARHTVDGLS
                     VELHAAYCRDALRLLLETCPAIGGLTVRTHGESGVAERSWDFWRTVLDGVATVGRRVS
                     VDLHAKGLDRETLDIALATGQPVTVSPKYSAEHMSLPYHQAAIRELDRPPVTGYDDRG
                     TKARYMAVCEGSRPFTRYSYGDLLREGRPYGVVYRIWPGTQRVLLWGDPAMAAGFSRA
                     ASLAGSQGLEWCEPLSLKGREGTGVPGGRSGYADDSLRPAQDWHKHAYSYRLFGRLLY
                     NPDAPSETWRRALRNDFGAAAESAEAALASASRILPLVTSAHHPSASNNYYWPEVYTD
                     IGIVSGPRTAETHYYDTPVPKRFGTVGPLDPEIFSSVQECVEEADAKLPSGRLSPWSV
                     AERLETLATEAAAQAAEALEECETPGSPDVRRLLVDVGILAALGRFFGSKLRSAVYYE
                     VASRTGSTACLRDAVTAYRSAHSAWTDAVRTADGVYVDNLTYGPQPWLRGTWSDRLAA
                     IDADLEAMESATAGGTATGDADSAALRILAAIMAGTSGHATHIPPAPFRPGEPVTVAV
                     KVGGDVEISRVSLRYRHLDQSATYEEVAMGRNGDGFSAAVPAAYADSPFALQYHFVIR
                     DTDGGSWHYPGLGPELSDQPYFVVRQREECRSRADGLPPSTS"
     gene            7352..>7890
                     /locus_tag="VH419_10125"
     CDS             7352..>7890
                     /locus_tag="VH419_10125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044012.1"
                     /translation="MDWYRQIQFVAAVAMPPDVARAYGTTVNGMWADPEQRSPEQVAD
                     DHRRGVRVLFSVPMIALVPRIYEADDTAYLLDEVCRDIHDDPAECDWYYWQPKPVYAA
                     CIYSDRFRTYLFDRCIEGVDKGMDVVNLDEIMTSIGLMDRDPGGTGFCHRCLDRFRGH
                     LLENGGGATATSSNAELRKR"
BASE COUNT         1303 a         2663 c         2575 g         1349 t
ORIGIN      
        1 acggcatcag gccggccagg gagccacgcc cggccatccg gtaccgaccg gagttgtaga
       61 tgatgatgag gtcggcgccg cccgcctccg cgcacttggc gctcaggccg gtgcctgccc
      121 ccgctccgac gaggactcca ccgtcgtcca gctgtgctcg gaaacgctcc aacacctgcg
      181 ccctcatgac cgggcctccc cgatcatcgc gtggagccgg ttcgccatcg cgagcgcgaa
      241 gtcagggtcg ttgacgtcgg tgtcgacttc gtggacctcg acattcggcc ccacggtctc
      301 gcgaatcgag gagaagagcg cgtcgtccgc cttcgagtcg tagaacggct gaccctccgt
      361 cgcgatcatc gacacgcccc gcaacggcac gaacaaggcc gtcggtccgg tggcggtgtt
      421 gagcttttgc gccacccggc gacccagctc ggcgcactcg tcgggcgtcg ttcgcatcag
      481 cgtgaccgta gggttgtgga cgtacaacgt ccgacttcgg aactgcgccg gcacggtgtc
      541 catcggcccg aagttgacca tgtcgagggc tccgagagaa accacctggg gaacgccgag
      601 cgagccggcg acctcgagcc gcttcgcatg tgcgcgaagc actccgccga cgagctcgtc
      661 gcagagctct gtggtggtga cgtcgagcac tcccttgatg aacccggctc tgacgagctc
      721 ctccatcgac tggccgccgg tgcctgtctg gtggaacacc agcacctcgt agctgagcga
      781 ctcgagccgt tcgcggccaa cggtcacgca cggtgtcgtc acaccgaaca tcgaggcacc
      841 gatgagcggc cgcatgtcgc ccagcgaagg tgcttcggcg ccggccatac cggccacggc
      901 cgcggcagca ttggcgatga tgcgggcgga gatctggttc acgccggcga cgtcgaccac
      961 cgagtacatc attgtgatgt cgaccgctcc gacgtacgga cgagtttccc cggaggcgac
     1021 cgtcgagacg acgagcttcg gtacgccgac agggagctgc tgcatcgcgt agctgacgag
     1081 cgacgtgcca cccgtgccgc ccataccgac gacggcgtcg agccggccct cgttgagaag
     1141 acgcgtcgcg accgcggctg ctccgcggct catcgcctcg atggcggcgc cacggtcgcc
     1201 cgccgccgcc aacgcggcga cgtcggcacc gaccgccccg gccacatcgt ttcgagagat
     1261 gtccgccgtc accgacggct ccccgatgat gcccgcgtcg acaaggacca cgtctgctcc
     1321 gtgcgcccgg atccgctcgc agaggaacgc gtactcggct cccttcgtgt cgagtgtccc
     1381 gatcaccaca acgttcggca ttcgacctcc ccggcatccg acacgctccg ggcgctcact
     1441 gcaggagcac cacggtgtac aacgggacgt tctcgagctc gacgcggtgc atctcgcgcc
     1501 gctcccggct gaacgtcagc cgtgccggga cttcccctgt cgggctgagc gcagacaccg
     1561 tacggaacgg ccgagagatc cggatgtcga ggctcagctt gtcgagcacc ggaacctcgt
     1621 cccgctcctc gtcgtagtcg tagcggatga tgtgaaccgc tgcctccttg tcgccgacgc
     1681 gggtgacgtt gagggcgagg tcgacaccag gctcgacaat cgcctgtgga cctccggtga
     1741 agtactcgct cctgacgtca gcagttcggg tgaggtgcgg atgggcgagc agcgctgaac
     1801 gctcaccagg gtcgagattc gctcccaggt cgccggtcgc gagcaccatg ccaccacgct
     1861 cgaggtagcc cagcagcgcc ttcgcctgga tcggggtgag gaacgtgcac tcgggcaaga
     1921 cgatggttcg gtagcgcgcc agatcgccgg cggtgaccca gtcctctcgc agctcgccct
     1981 ccggcagcac gatgacgtcg aacggctggt gctgctcgac cagggcctcg gtggcggtcc
     2041 agaacgggaa cttcaccttc cagcccttgt gctcctccca gtcgtaggcg ctttgaacgc
     2101 tgtagaggac gacggcttcg gaataggtct ctggctcgta gagccgctcg ttggttgcga
     2161 tgaagtcctg gatctcgaca ttcagctcgt gtggcgccca gaaggagtct tcgatgacgc
     2221 tccccatcca agcgccgtac ggcacgctca tgttgatgcc cagggcggac gcctcgtact
     2281 gcatcatgcg gtacaggtcg tagccctttc ctgcctgcag cttcggcaac agctccgggc
     2341 ccaccccgcc atacgggttc tcgacgacga tgaccggctt gcctcgtgcg aagccggcgg
     2401 cgtaccggca ccagttcggt tggcggtagc tggtcgcgtt catctcggtg acgaggatgt
     2461 ccgccttcgg ctcgaacggg tagtagtgcg gtgagaggta gtagtagttg ccgcacacca
     2521 ggacgtcgcg ccctttcgag cgtccgtact ccttgatgta gtcggccagc tcggcgaagt
     2581 agccgacgat cgtcttgcgc tggaaccgga tgtagtccca gtacaaggga gtggcctctc
     2641 ggtcggactt gaagtcgtac ccacgctcga gcagccagct gccgtagtgg aacgtgtcga
     2701 ggtcggtgtc cttcaacggt tccggcagcc ggtcggacga cagttcctgt aggtacgccg
     2761 tgaacagctt catgcagtcc ttgcagaagc agccgccata tccggtggcg aagatcggca
     2821 gatcggcctc gtccaggtgg acgccggcca caccggcgtc gatctggatc cggatgatcg
     2881 ccttcaggta ctcgcgccac acggggttgt tgcggtccat caggtacgcg tagtgatcgt
     2941 tgcccggtac ctcgacccag gtggaatgac acgcgttccc gtggatgtcg cgtgacgtgc
     3001 actcctcgag gatgtcggtg actgtctcgc cgtcgctgtt caccagcccg tccgggaagt
     3061 actcctcgag cgccttccag atcttcggat accggttctg cgtgaactcc ctcaagccga
     3121 gccagccccg tttgcccacg ccccggacct cgttcatcga caggaccgtc gactcgtcgt
     3181 cgtcgagctc gaccgggaac tcccagccct gtacttcgaa aacgcaaccg aagaccttga
     3241 tgccacggtc ctgacagtgc ttgatgaact ccgcgtcgtt gacgaagccg tagaaccgga
     3301 atctttcgtc gacttctccc catgcttcct gttcgaggta cggcagcgag atcgatccgc
     3361 cgccgagggc tgaccagacg agactggtcg cgtggtacgc ctcgaggtcg tcgatcatcc
     3421 gcaggcggcg cggaggaaga ctcgggtggt agcagtgctg acgaggaaac catgcgtcga
     3481 agtagacacc gcgttccatg ctctaggccc tcccttggcc gtggatctgc ggatcaggcg
     3541 ccggaccagt cgagcaccat cccgagtgac tgttccgggt ggtcgacgac gagctcgtat
     3601 acctggtggg cgtcgcgata gggcatgcgg tgcgtgatca aggggtcgat cgcgatgctg
     3661 cccttcgcca tgtcatcggc caggtcgtcg ttgaactcgt cgtcccaccc gcagggaaag
     3721 accacgtgtg ggcgcttgag gtgggtgggg tggaagtcga tcgagatcgg gtcggggtag
     3781 tacccctgca tcacgatctt gccttcgtac ttcaccagat cgaggcatcg gtcgaacatc
     3841 cttgccacac cggtcgtgtc gatgacgacg tcggcgccgt ccgggtgctc tgtgcggacg
     3901 acctcctcaa gcgactccgc ctcaggatcg acgcctcggt ccgcggagtg ccgtgcggcc
     3961 gcctcgaccc gcaaccggat cagatcggtc gccactactc gagcaccggc atgtcgtgcc
     4021 gcctgcgccg acatctggcc gatcatcccc agcccgatca cgaccacgag gtcgtcaggc
     4081 tcgacgccgg tcagccggac gccatgccgg gacacgcccg ccatgtggaa cagcgacgcg
     4141 ccggcgaggt ccacgcccgg gtcgacccgc cacacctctg ctgcattggc gacgaggtac
     4201 gcggaatgcg acgcctggca tggaccgggg tacttgtgca cgggatccgc gaagtgggtc
     4261 cccatcgtga agacgtgatc ccccgtcgcc aggctgtcga cctcggagcc gacgacgtcg
     4321 acgacaccgg cggcttgata gcccggcgag cagggatagt acgccgagta gtcgtggtcg
     4381 tagtgcccgt accggccggt gagcgcccag cgctccgtgc cctgactgac gccggagaac
     4441 gctgtgcgga tgcctacctg gccgggtccg acttcgggga gatccatctc tttgatctcg
     4501 atgcgcttcg gctcggcaaa cacgatggcg ggcgccctca tggcaccgct ccttccctgg
     4561 cggtccggcg gaggctcggg ttggtgtgag aacgatacca caccgcgctc atgacgtgga
     4621 cggcggtagt ccgtcggctc gcgacctgca ttcctcccgc tgcctgacga cgaaatacgg
     4681 ctgatcggac agctcagggc cgagccctgg atagtgccac gagccgccgt cggtatcgcg
     4741 gatcacgaag tggtactgga gggcgaacgg cgagtcggcg tacgccgcag gcaccgcggc
     4801 tgagaacccg tcgccatttc ggcccatggc gacttcctca tacgttgccg actggtccag
     4861 gtggcgatac cggagtgaca cgcgcgagat ctcgacgtcg ccaccgacct tcacggccac
     4921 cgtcaccggc tcgcccggtc ggaacggtgc gggcggtatg tgcgtcgcat gaccgctcgt
     4981 cccggccatg atggccgcga ggatgcgtag cgccgcggaa tccgcgtcac cggtggccgt
     5041 gccgcccgcg gtggccgact ccatggcctc gaggtctgca tcgatcgccg ccaagcggtc
     5101 ggaccaggtc ccgcgcagcc atggttgcgg tccgtatgtg aggttatcga cgtagacgcc
     5161 gtcggccgtt cgcaccgcgt cggtccatgc ggagtgcgct gaccggtacg cggtcaccgc
     5221 gtcacgaagg caagcggtgc tcccggtcct gctggcgact tcgtagtaca ccgcggacct
     5281 gagcttcgac ccgaagaacc tgccgagcgc ggccaggatt ccgacgtcga cgagcagccg
     5341 tcgcacatca ggtgatcccg gcgtctcaca ttcctccaac gcctccgccg cctgtgcagc
     5401 tgcctcggta gcgagcgtct ccaatcgctc ggcgaccgac cagggtgaga gtcgtccgct
     5461 gggcagtttg gcgtcggctt cctcgacgca ctcctgcacg ctcgagaaga tctccgggtc
     5521 gagcggcccg accgtgccga accgcttcgg gacgggagtg tcgtagtagt gcgtctcggc
     5581 ggtgcgtggg cccgaaacaa tgccgatgtc ggtgtagacc tcgggccagt agtagttgtt
     5641 cgaggccgac gggtggtgcg cgctcgtcac gagcggcagg atgcgactgg cgctggcgag
     5701 cgccgcctcc gcggactcgg cggcggcgcc gaagtcgttc cgaagggcgc gacgccaggt
     5761 ctccgaaggc gcatccgggt tgtacagcag ccgtccgaac aggcggtacg agtaggcgtg
     5821 cttgtgccag tcctgcgccg ggcgcaacga gtcgtcggcg tacccgctgc gaccgcccgg
     5881 gacgccggtc ccctcacggc ccttgaggga cagtggttcg caccactcga gcccctggct
     5941 ccccgccagg cttgctgcac ggctgaagcc tgctgccatc gccggatccc cccacaagag
     6001 gactcgttgt gttccgggcc agatccgata caccacgccg tacggtcgcc cctcgcgcag
     6061 caggtcgccg tagctgtatc gggtgaaggg gcggctcccc tcgcacactg ccatgtagcg
     6121 ggccttcgtc ccgcggtcgt cgtagcccgt gaccggcggt cggtcgagct ccctgatggc
     6181 ggcctggtga taggggaggc tcatgtgctc cgccgagtac ttcggcgaca ccgtaacggg
     6241 ctgccccgtg gcgagcgcga tgtcgagggt ctcgcggtcg agcccttttg cgtggaggtc
     6301 cacactgacg cggcgtccca ccgttgccac cccgtcgagc accgtccgcc agaagtccca
     6361 gctgcgttcg gcgactccgc tctccccgtg cgtgcgcacc gtcagtccgc cgatcgccgg
     6421 gcaggtctcg agcagcagcc ggagcgcgtc gcgacagtac gccgcgtgca gctcgacgga
     6481 gagcccatcg acggtgtgcc tcgcatgagg actgtcgaac cattcgtacg catgggtcca
     6541 gagaccgacc tggaagtcca gcccgcgcac caccgcctgc tcggacgcaa actgcagcat
     6601 cgccaggttc cggtcgcgct cctcgtccgg aagctgcggt atccgcacgc gatagccggg
     6661 cacgtcgagc aggaacggat acgggaagaa caagtaggcg tcggtgacgt gccagggaaa
     6721 gttgtagccg agcccgacca tcaggttgac gcggttgaac cgctgggtca ccgccatggt
     6781 caggtagtcg tgccagaacg cctcgtcctg cagccaccgt ttgtcgtcga cctcactggc
     6841 gaagcagcgg gcaacgctgc gcaccgcgtt ggcgggctgc tcgatcgtcg ccgtgtcgaa
     6901 acgcatcgcc tcgatcggat ccgctgcgtg ctcgacccga tcggcgagct ccaggatcgc
     6961 gtacaccaat ccgcgctcgt cgtgaccggc cgccagtaga cgacgttgcc cctctgactc
     7021 gaacggtaca agcgcaagcg actcgggcac ggtcggcacc gccagcccca accgcgacaa
     7081 gatctcctgg ctgcggccgt gatcggcgcc cccgatcagg atggccaggt cggccaccgg
     7141 atccggctgt tcggtgcgat cggccattgc ccccttcgcc gcaagggccg cttgcagccg
     7201 gcgggtcgcc cagtccaccg gagtcaggcc ggcgagccgg tcatcgcgac cgcacgcaag
     7261 cgagatgcgc ggtgaggtgt tcaggaaatg tggaaacgat atcatactga ctgtcgtcga
     7321 aacgccagcc agccagacca gaggaggcga cgtggactgg taccggcaga tccagttcgt
     7381 cgccgcggtt gcgatgccac ccgacgtcgc gcgcgcgtac ggcaccacgg tgaacgggat
     7441 gtgggcagat cccgagcagc gctctccgga gcaggtcgcc gacgaccatc ggcgcggagt
     7501 cagggtgttg ttctcggttc cgatgatcgc gctggtaccg cgcatctacg aagccgatga
     7561 cacggcgtac ctgctcgatg aggtgtgccg agacatccac gacgacccgg ccgagtgcga
     7621 ctggtactac tggcagccca agccggtcta cgccgcctgc atctacagcg atcgcttccg
     7681 cacgtacctc ttcgaccgct gcatcgaagg cgtcgacaag ggcatggacg tcgtgaacct
     7741 cgacgagatc atgacgtcga tcggattgat ggaccgtgat ccaggcggga caggcttctg
     7801 ccaccgttgc ctggaccgat tccgcggcca tctcctcgag aacggcggtg gcgccaccgc
     7861 gacgtcaagc aatgccgagc tccgcaagcg
//
LOCUS       DATBFZ010000327         6480 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_5364216, whole genome shotgun sequence.
ACCESSION   DATBFZ010000327 DATBFZ010000000
VERSION     DATBFZ010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 6480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6480
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5364216"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1015)
                     /locus_tag="VH419_10130"
     CDS             complement(<1..1015)
                     /locus_tag="VH419_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009079479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEY7044013.1"
                     /translation="MSHRKAPRTSHALPPDPGWFKRAVFYEVLLRSFKDSDGDGVGDF
                     RGLREKLDYLKWLGVDCLWIPPFYSSPLRDGGYDISDFTDVQPDVGTLDDFQRLVDDA
                     HQLGLRIIIDFVMNHTSDQHPWFQESRRDPDGPFGDFYVWSDNDELYEGARVIFVDTE
                     PSNWTWDSARQQYFWHRFYSHQPDLNYDNPKVHDAILDALRFWLDMGIDGFRLDAVPY
                     LYERPATNGENLPETHDFLRKVRKAVDDEYPGRVLLAEANQWPADVVDYFGDFEQGGD
                     ECHMCFHFPVMPRIFMAVRRESRYPISEILEQTPAIPDNCQWGIFLRNHDELTLEMVT
                     DEDR"
     gene            complement(1030..3039)
                     /locus_tag="VH419_10135"
     CDS             complement(1030..3039)
                     /locus_tag="VH419_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012922511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,4-glucan--maltose-1-phosphate
                     maltosyltransferase"
                     /protein_id="HEY7044014.1"
                     /translation="MIERIPVTDVSPVVEHGRYPVKATVGETFTVSATVFREGHDALA
                     AGVVLTDPKGKDRPLVRMAAVGASQPDRYAAEVTPDAEGSWTYRIESWGDPYASWRHA
                     AEIKIPAEIDVELMFSEGARILSRAAKSIPAKAKELRPTLTAAAKAGRDRKRPVAVRL
                     AAMTSPEVLDILYRHPLRDLVSRHGPYPLYVDRVRALYGSWYEFFPRSEGAAYDDSTQ
                     AMRSGTFTTAAKRLDAIAEMGFDVVYLPPIHPIGEVNRKGPNNTLTPGPHDPGSTWAI
                     GSKEGGHDAIHPDLGTMDDFEAFVSRARQLGLEVALDLALQCAPDHPWAVDHPEWFTT
                     RADGSIAYAENPPKKYQDIYPLNFDNDFEGLSREVRRVVRHWMDHGVRIFRVDNPHTK
                     PVMFWEWLLEDIRRTDPDVIFLSEAFTRPAMMRGLAMIGFHQSYTYFTWRNAKWELEQ
                     YFYELSHLASDVMRPNLFVNTPDILTDYLQYGGPAAFRVRATLAATASPTWGVYSGFE
                     LYEHVAVKPGSEEYLDSEKYQFRPRDWDGAEREGRTLAPYITKLNLIRRAHPALQALR
                     NLTVHGSEDDAVLCYSKRTVTRGEASADASDEFDDTVIVVVNVDPHGTRETMVHLNMP
                     ALGYDWHDTFVVEDLITGQYWRWGETSYVRLDPSYEPAHVLAIRK"
     gene            3184..3432
                     /locus_tag="VH419_10140"
     CDS             3184..3432
                     /locus_tag="VH419_10140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044015.1"
                     /translation="MTTDLPDPQQPTIDEEPDRAPGGADADLSESDVGAASRGTPVTP
                     DVPLSAQQDQAEVPDEVQAPEPPETTDHGDDGPSEPSA"
     gene            complement(3874..4419)
                     /locus_tag="VH419_10145"
     CDS             complement(3874..4419)
                     /locus_tag="VH419_10145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044016.1"
                     /translation="MSKPTYRVVKLAPGYDLGAFDCGEPFYNEWLIRHAAPSVQAGVC
                     AVYLLLEEVEDQVRIVGYYSINPTQVVREVVPAKMTRGWPRTVPAWKIGKLALHVDLR
                     ENKDAEWGRQLLREALETVVRVADVGGGKVIVIDADSPGLLGFYGRNGFKSTGIEGDL
                     SMYMKVSSAKKVLNVPVRSEA"
     gene            complement(4416..4715)
                     /locus_tag="VH419_10150"
     CDS             complement(4416..4715)
                     /locus_tag="VH419_10150"
                     /inference="COORDINATES: protein motif:HMM:NF020265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HEY7044017.1"
                     /translation="MATTKAPSKARKARPKARLEARIDPELDDLIAEAAERLRVNKTT
                     FVSDAVREAALKVLAHADVTMMEPDVFDAMMASIDAADESAELESLAAMPRLITR"
     gene            4890..>6480
                     /locus_tag="VH419_10155"
     CDS             4890..>6480
                     /locus_tag="VH419_10155"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEY7044018.1"
                     /translation="MVDLRWLPGSSAVALCLVLALAATGCTSDHSGSAANGADEPAAP
                     ADTLVTGKSMTLDVLVYNIEYGGNASTDKVIRSVDADVVGVLESYNRLPEIARRTGYP
                     YYNVELQLLSKYPILEPSGADGLYALLEVRPGYVVAFFNTHLDYVAYGPRLLASGMPV
                     PDVLASEDEVRTSSMRILLPDLKELAAAGYPTFLTGDFNEPSSLDYTHDTVGMHAGVH
                     EAVSWPVSKALLDIDFRDSYREIHPDPAAVPGNTWGNLNGEQHIAADRIDYVYSGGPV
                     EATASRLVGEQGGPDVDIGFRRWTSDHRAVVSTFDLSPVETPTTVSLDTRMLTRGDTL
                     TVRYNSVTTSPATITVRRGGDGQANETPQDNAGGEDGVVTQRQVTGDSGTLTFPTRRF
                     APGGYEVAMDAADGSEIAQNEFWVRSRHPDVVLSCDRSAYAVNEPVTVTWDDGPANRW
                     DWVAVYRAEAADPHKDPYLLWGYTGGHNSGALPPTVSGSITFDASSQGKLWPLPPGKY
                     VVRYLLTDQYQSVGRATFTVKR"
BASE COUNT         1059 a         2102 c         2178 g         1141 t
ORIGIN      
        1 cgcggtcctc gtcggtgacc atctcgagcg tcagctcgtc gtggttgcgc aggaagatgc
       61 cccactgaca gttgtcgggg atggccgggg tctgctcgag gatctccgag atcgggtagc
      121 gcgactcccg ccggacggcc atgaagatgc gcggcatgac cgggaagtgg aagcacatgt
      181 ggcactcgtc gccaccttgc tcgaagtcac cgaagtagtc gacgacgtcg gccggccatt
      241 ggttcgcctc ggccagcagg acccggccgg ggtactcgtc gtcgacggcc ttgcggacct
      301 tgcggaggaa gtcgtgtgtc tcggggaggt tctcgccatt cgtggccggc cgttcgtaca
      361 ggtacggcac cgcgtcgagg cggaagccat cgatgcccat gtcgagccag aagcgcaacg
      421 cgtcgaggat cgcgtcgtgg acctttgggt tgtcgtagtt caggtccggc tgatgactgt
      481 agaagcggtg ccagaagtac tgctggcggg ccgagtccca tgtccagttg ctcggctcgg
      541 tgtcgacgaa gatgacgcgt gcgccctcgt agagctcgtc gttgtcggac cacacgtaga
      601 agtcgccgaa gggtccgtcg gggtccctgc gcgactcctg gaaccacggg tgctggtcac
      661 tggtgtggtt catgacgaag tcgatgatga tgcgcagccc gagctggtgg gcatcgtcga
      721 cgagtcgctg gaagtcgtcg agcgtcccga cgtccggctg gacatcggtg aagtcggaga
      781 tgtcgtagcc gccgtctcgt agcggcgagc tgtagaacgg cgggatccag aggcagtcca
      841 cgccgagcca cttcaggtag tcgagcttct cgcgcagccc acgaaagtct ccaacaccgt
      901 ctccgtcgga gtccttgaac gacctcagca gcacctcgta gaagaccgcc cgcttgaacc
      961 accccggatc aggtggcagg gcgtgcgacg tccgcggcgc ctttcggtgc gacatatcgt
     1021 ccgtcatggc tacttcctga tcgccagcac atgcgcaggc tcgtacgacg ggtcgaggcg
     1081 cacgtagctg gtctcgcccc atcgccagta ctggcccgtg atcaggtcct cgacgacgaa
     1141 cgtgtcgtgc cagtcgtagc cgagcgccgg catgttgagg tgcaccatcg tctcgcgcgt
     1201 tccgtgcggg tcgacgttga ccacgacgat gacggtgtcg tcgaactcgt ccgacgcatc
     1261 cgcggacgct tcgcctctgg tgaccgtgcg cttgctgtaa cagaggaccg cgtcgtcctc
     1321 gctgccgtgc acggtgagat tgcgcagcgc ctgcagggcc ggatgggccc gtctgatcag
     1381 gttgagcttg gtgatgtacg gcgccaacgt cctgccttcg cgctcggcgc catcccagtc
     1441 acgcggccgg aactggtact tctccgagtc gaggtactcc tcggaaccgg gcttgaccgc
     1501 cacgtgctcg tacagttcga aaccggagta cacgccccag gtcggggacg ccgtcgcggc
     1561 gagggtcgcc cggacgcgga acgccgccgg cccgccgtac tggaggtagt cggtgaggat
     1621 gtccggagtg ttgacgaaga ggttcggccg catcacgtcg ctggcgaggt gcgacagctc
     1681 gtagaagtac tgctcgagct cccacttggc gttgcgccac gtgaagtacg tgtaggactg
     1741 gtggaacccg atcatcgcga gaccacgcat catggccgga cgggtgaacg cctcggacaa
     1801 gaagatgacg tccgggtccg tccggcggat gtcctcgagc agccactccc agaacatgac
     1861 cggcttcgtg tgcgggttgt cgacccggaa gatgcggacg ccgtgatcca tccagtgacg
     1921 gacgactcgt cgcacctctc gtgacaggcc ctcgaagtcg ttgtcgaagt tgagcgggta
     1981 gatgtcctgg tacttcttgg gagggttctc ggcgtacgcg atcgacccgt cggccctcgt
     2041 cgtgaaccac tccgggtggt cgaccgccca tgggtggtcc ggggcgcact ggagcgcgag
     2101 atcgagcgcg acctcgaggc cgagctgacg tgcgcgggag acgaacgctt cgaagtcgtc
     2161 catcgtcccg agatccgggt ggatcgcgtc gtggccgccc tccttcgacc cgatcgccca
     2221 cgtcgagccg gggtcgtgcg ggccgggtgt gagggtgttg ttcggcccct tgcggttcac
     2281 ctcaccgatc gggtggatcg gcggcaggta gacgacgtcg aagcccatct cggcgatggc
     2341 gtccagccgt ttggcggcgg tcgtgaaggt gcccgaccgc atcgcttggg tgctgtcgtc
     2401 gtacgcggcg ccctccgagc gagggaagaa ctcgtaccac gagccgtaca acgcccggac
     2461 ccggtcgacg tacaacgggt agggcccgtg cctggacacg aggtcgcgca gcggatgccg
     2521 gtacaggatg tccaggacct ccggcgacgt catcgcggca agccggacgg cgacgggtcg
     2581 cttgcggtca cgtccggcct tggcggcggc cgtcaacgtc gggcggagct ccttcgcctt
     2641 ggccgggatg gacttcgcgg cgcgggagag gatccgggcc ccctcgctga acatcagctc
     2701 gacgtcgatc tccgcgggga tcttgatctc ggcagcgtgc cgccagctgg cgtacgggtc
     2761 accccaggac tcgatccgat acgtccacga tccctcggcg tccggtgtca cttccgcggc
     2821 gtaccggtcc ggctggctcg cgcccaccgc ggccatcctg accagtgggc ggtccttgcc
     2881 cttcgggtcg gtgagcacca cacctgcggc gagggcgtcg tgcccctcgc ggaagaccgt
     2941 ggcgctgacc gtgaaggtct ccccgacggt cgccttgacc gggtaacggc cgtgctcgac
     3001 caccgggctg acatccgtga ccggaatacg ctcgatcatc acatcgaacc tagcgaaccg
     3061 ccctctccgc tgcacacccc ggctgcacac cggctgcaca ccacgttgca ggcacggttc
     3121 cgacgcaaaa tgaacaaggg ctgacgggtg tgccacccgc ggcgatcggg aacgggtgct
     3181 ggtatgacca ccgatctccc ggacccccag cagccgacga tcgacgagga gccggaccgc
     3241 gcgccaggcg gcgccgacgc ggacctgagc gagagcgatg tcggagcggc gagcaggggt
     3301 accccggtga ctccggatgt cccgctgtcc gctcagcagg accaggccga ggttcctgac
     3361 gaggtgcagg ccccggagcc gccggaaacg accgaccacg gcgacgacgg cccgtccgag
     3421 ccgtcggcct gaccgaaccc agctggctcc tcgtgagcac tcaggtgggg agcgccatct
     3481 tcaccacccg ccggacaggc gtccatcgtg gtttgcatgg ttgtgcaacg ggcgggtagc
     3541 cgggcagaac tgggtctcct ccggtctgtg tgggcgcaga ggtcgcgatg ggtgggcgtg
     3601 gtcgcccggc gctgcgccgg cgccggctga ggtggatccg cagatatgag cggaggtatt
     3661 gctgcctact gagctggttg ccggctttcc cgcaccttct tgaatgccgt gtggttagcg
     3721 ggtttcggac atctgcggtc gctgagcggc ggtctgcatg gctacccagg caatcgcacc
     3781 cgtcgcaagg cagatctgcc tggctacccg tccgtttgca tggctaccga ggcaaaccac
     3841 aaccgaatgg tcggcgtctt gagattggat gcgctatgcc tcagaacgga ccggcacgtt
     3901 gagtaccttc ttggcagatg agaccttcat gtacatcgac aggtcgccct cgatgccggt
     3961 gctcttgaac ccgtttcggc cgtaaaagcc aagcagacct gggctgtcgg cgtcgatcac
     4021 gatgactttt ccgccgccga cgtcggcgac tcgcaccacc gtctcgagag cctcgcgaag
     4081 gagttgccgc ccccactccg cgtccttgtt ctcacgcaga tcgacgtgca gggcaagttt
     4141 gccgattttc caggccggca ccgtccgcgg ccatccccgg gtcatctttg cgggaacgac
     4201 ttcgcggacc acctgggtgg ggttgatcga gtaatagccc acgatgcgga cttggtcctc
     4261 gacctcttcc aagagcagat agaccgcaca cacaccggct tgaactgagg gcgctgcatg
     4321 cctgatcagc cactcgttgt agaagggctc accacagtcg aaggccccca agtcgtaacc
     4381 gggggccagc ttgacgactc ggtatgttgg tttgctcacc tggtgatcag gcgcggcatg
     4441 gccgccaggc tttcgagctc ggccgactcg tccgccgcgt cgatggaagc catcatcgcg
     4501 tcgaaaacgt cgggttccat catcgtcacg tcagcgtgag cgagcacctt cagcgcggcc
     4561 tcgcggacag cgtcagaaac gaacgtggtc ttgttgacgc gcagccgttc ggcagcctcg
     4621 gcaatcaggt catcgagctc tgggtcgata cgtgcctcga gacgcgcctt gggtcgggct
     4681 ttgcgtgctt tggacggggc cttggtcgtg gccatgcggt ctgcacctcc aaacaacaac
     4741 tgtacggcag ttgtacggca ctttcaagcc ggtggttacg agtctcgact cattcgcgcc
     4801 ggcgcagtgc gggtgatgct ggaccgcctg ccgtgcggcg gcaatcatga ggacacccgt
     4861 tcgcgggttc cctttcagga ggctgcctcg tggtcgacct gcgctggctc ccgggctcat
     4921 cggctgtggc gctgtgtctc gtgctggcgc tcgccgcgac gggatgcacc tcagatcact
     4981 cgggcagcgc cgccaacggc gccgacgagc cggctgcgcc ggccgacacg ctcgtgaccg
     5041 ggaagtcgat gacgctcgac gtcctcgtct acaacatcga gtacggcggc aacgcgtcga
     5101 ccgacaaggt gatccggtcg gtcgacgctg acgtcgtcgg agtcctcgag tcgtacaacc
     5161 ggctgccgga gatcgcccgg cggaccggct atccctacta caacgtggag ctgcagctgc
     5221 tgtcgaagta cccgatcctc gagccatcgg gtgctgacgg cctgtatgcg ctgctcgagg
     5281 tgcggcccgg ctacgtcgtc gcgttcttca acacccatct cgactacgtc gcctacggcc
     5341 cgaggctgct ggcatctggc atgccggtcc ctgacgtcct ggcctccgag gacgaggtgc
     5401 gcacgtcctc gatgcgcatc ctgctgcctg acctgaagga gctggccgcc gccggctacc
     5461 cgacgttcct gacgggggac ttcaacgagc cctcaagtct ggactacacc cacgacacgg
     5521 tcgggatgca tgccggagtt cacgaggcgg tgtcgtggcc cgtgagcaag gcgctgctcg
     5581 acatcgactt ccgcgactcc tatcgcgaga tccatcctga tcccgcggcg gtcccgggca
     5641 acacgtgggg gaacctgaac ggcgaacagc acatcgctgc tgaccggatc gactacgtct
     5701 actccggcgg tccggtggag gcgaccgcga gccggttggt cggagagcag ggtggtcctg
     5761 acgtcgacat cggcttccgc cgctggacgt cggaccaccg tgctgttgtt tcgacgttcg
     5821 acctgagccc ggtggagacg ccgaccacgg tgtcactcga cacccgcatg ctgacgcgtg
     5881 gcgacaccct cacggttcgc tacaacagcg tgacaaccag cccggcgacg atcacggtgc
     5941 ggcgcggagg cgacggccag gcgaacgaaa cgcctcagga caacgccgga ggcgaggacg
     6001 gcgtggtgac ccagcggcag gtgaccgggg actcagggac cctcacgttc ccaacgcgta
     6061 ggttcgcccc gggtggctac gaggtcgcga tggacgcggc cgacggcagc gagattgcgc
     6121 agaacgagtt ctgggttcgc tcgcggcatc ccgacgtggt gctgagctgt gaccgatcgg
     6181 catacgccgt gaacgaaccc gtcacggtca cgtgggacga cggtccggcc aaccgctggg
     6241 actgggtggc ggtgtatcgc gccgaggccg ccgatccgca caaggatccc tacctgctct
     6301 gggggtacac cggaggacac aactccggtg cgctcccgcc cactgtcagc gggtcgatca
     6361 ccttcgacgc gtcctcgcag ggcaagctgt ggccactgcc accggggaag tacgtcgtgc
     6421 ggtacctcct caccgaccag taccagtcgg ttgggcgggc caccttcacg gtcaagcgtt
//
LOCUS       DATBFZ010000328         2466 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_3504391, whole genome shotgun sequence.
ACCESSION   DATBFZ010000328 DATBFZ010000000
VERSION     DATBFZ010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3504391"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(164..334)
                     /locus_tag="VH419_10160"
     CDS             complement(164..334)
                     /locus_tag="VH419_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012920643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HEY7044019.1"
                     /translation="MGSTPKEPEKAQFNVYLPPDLIRRTKHKAIDEGSSLSSLVERAL
                     EEFLENHGEAKG"
     gene            complement(594..1769)
                     /gene="ispG"
                     /locus_tag="VH419_10165"
                     /gene_synonym="gcpE"
     CDS             complement(594..1769)
                     /gene="ispG"
                     /locus_tag="VH419_10165"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012920636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEY7044020.1"
                     /translation="MTAIDLGMPSAPPPVLTPRRKTRQIKVGKVLVGGDAPVSVQSMT
                     TTVTAEVDTTLQQIAELTASGCDIVRVACPSQDDADALPEIARHSQIPVIADIHFQPK
                     YVFAAIEAGCAGVRVNPGNIRKFDDQVKEIAQAAKDHGTSIRIGVNAGSLDPRLLEKY
                     GKATPEALVESAVWEASLFEEHDFHDFKISVKHNDPVVMVRAYELLSEAGDWPLHLGV
                     TEAGPAFQGTIKSSVAFGALLSQGIGDTIRVSLSAPPVEEVKVGLQILESLNLRARRL
                     EIVSCPSCGRAQVDVYKLADEVTAGLEGMEVPLRVAVMGCVVNGPGEAREADLGVASG
                     NGKGQIFVKGEVIKTVPESMIVETLIEEAMRIADEVGVPVDENGGSVAAGSPTVIVS"
     gene            complement(1798..>2466)
                     /locus_tag="VH419_10170"
     CDS             complement(1798..>2466)
                     /locus_tag="VH419_10170"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HEY7044021.1"
                     /translation="QFSDKVRANAGGHIDVLVDRDGSQLTLSTNTVVSERPDLDEPGK
                     YVAVGFLGVLPTEIREHKDLGYVASTMGDYTAKTGQALLHLPQRMVGVAQAALGTESR
                     DPNSPISVVGASRVAGEVASEDTISVTDRWATLLTLLGVVNLFVALFNFIPLLPLDGG
                     HIAGALYEALRRGIARLRGRPDPGYVDVAQLLPIAYTAATVLIAMSVLLVWADIVNPI
                     RISG"
BASE COUNT          398 a          847 c          807 g          414 t
ORIGIN      
        1 gcgtcatgga gcgcgaccat cccgctgtcg gcaatcagat cgacccagcc gcctcggtca
       61 gactcgatcc gtgggcgaag tccgatggtc tcgaggaacg cctgcattcg cggcacgtcc
      121 gacgagaaac gaaccggtcg aacgacgatg ttgctcatgc cggtcacccc ttggcctctc
      181 cgtggttctc gaggaactcc tccaacgctc gttcgacgag cgacgacaga ctgctgccct
      241 cgtcgatcgc cttgtgcttg gtacgccgga tcaggtctgg cggcaggtac acgttgaact
      301 gcgccttctc cggttccttc ggcgtcgaac ccatacctag aatgctagca acctagtttc
      361 gaggcttgtc aatcgcggcc acccaggccg acaaccgttg acacgcgccg gtggtggttc
      421 gcctcggtag ccatccgaat gcacgggtag ccaggcaggt ctgccttgcg agccatgcgt
      481 ttgcacggct acccaggcaa accggggacc ggtcaacgac ggatgtccga aacccgcgag
      541 tcacaggtga ggcagacccg ggcgaaacgc cggcctgacc ggaccgggac cgactaggag
      601 acgatcaccg tcggggatcc ggcagcgacg gacccgccat tctcgtcaac cggtacgccg
      661 acctcgtcgg cgatccgcat cgcctcctcg atcaacgtct cgacgatcat cgactccggc
      721 acggtcttga tgacctcgcc cttgacgaag atctggccct tgccgttgcc ggacgccaca
      781 ccgaggtcgg cctcgcgggc ctcgcccggg ccgttgacga cacaacccat cactgcgacc
      841 cgcagcggga cttccatgcc ttcgagaccg gcggtcacct cgtccgcgag cttgtagacg
      901 tcgacctgcg cccggccgca cgacggacac gacacgatct cgagccggcg cgcccgcagg
      961 ttcagcgact cgaggatctg caggccgacc ttgacctcct cgaccggcgg cgcggacaac
     1021 gagacccgga tcgtgtcccc gatgccctgc gacagcagcg cgccgaacgc caccgacgac
     1081 ttgatcgtgc cctggaacgc cggaccggcc tccgtcacgc cgaggtgcag cggccagtcg
     1141 cccgcctcgg acaacagctc gtacgcccgc accatgacga ccgggtcgtt gtgcttgacc
     1201 gagatcttga agtcgtggaa gtcgtgctcc tcgaacagcg acgcctccca gacggccgac
     1261 tcgacgagcg cctccggggt cgccttgccg tacttctcca gcagccgcgg gtcgagggag
     1321 cccgcgttga cgccgatcct gatcgaggtc ccgtggtcct tggccgcctg cgcgatctcc
     1381 ttgacctggt cgtcgaactt cctgatgttg cccgggttga cacgcacgcc ggcacagccc
     1441 gcctcgatgg ccgcgaagac gtacttcggc tggaagtgga tgtcggcgat gacggggatc
     1501 tgcgagtgtc gcgcgatctc gggcagcgcg tcggcgtcgt cctggctggg gcacgcaacc
     1561 ctgacgatgt cacagccgga ggcagtgagc tccgcgatct gctgcagtgt cgtgtcgacc
     1621 tcggcggtga cggtggtcgt catcgactgg acggagaccg gcgcgtcgcc gccgacgagc
     1681 accttgccga ccttgatctg gcgcgtcttg cgacgcggtg tcagcaccgg cggcggcgct
     1741 gacggcattc cgaggtcgat ggcggtcatg cagattcctt cggggtggcg gtgccggtca
     1801 tccgctgatc cggatcgggt tgacgatgtc ggcccacacg agcaggacgc tcatcgcgat
     1861 gagcacggtg gccgcggtgt aggcgatcgg gagcagctgg gccacgtcga cgtacccggg
     1921 atctggccgc ccgcgcagcc tcgcgatgcc ccggcggagc gcttcgtaga gcgcgcccgc
     1981 gatgtgtcca ccgtcgagcg gcagcagcgg gatgaagttg aagagggcca cgaacaggtt
     2041 gacgacaccc agcagggtga gcaacgtcgc ccaccggtcg gtcacgctga tcgtgtcctc
     2101 ggatgcgacc tcgccggcga cccggctggc tccgaccacg ctgatcgggc tgttcgggtc
     2161 gcggctctcg gtacccagag cggcctgcgc gacccccacc atccgttgcg gcaggtgcag
     2221 cagggcctgc ccggtcttgg cggtgtagtc gcccatcgtg ctggcgacgt agccgaggtc
     2281 cttgtgttcg cgtatctcgg tcggaagtac gccgaggaaa ccgaccgcga cgtacttccc
     2341 gggctcatcg agatccggcc gctcggacac cacggtgttc gtcgacagtg tcagctggct
     2401 gccgtcccgg tcgacgagga cgtcgatgtg accgccggcg ttcgcgcgca ccttgtcgga
     2461 gaactg
//
LOCUS       DATBFZ010000329         1734 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_4159499, whole genome shotgun sequence.
ACCESSION   DATBFZ010000329 DATBFZ010000000
VERSION     DATBFZ010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4159499"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            240..704
                     /locus_tag="VH419_10175"
     CDS             240..704
                     /locus_tag="VH419_10175"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY7044022.1"
                     /translation="MPDQNVSTSRQVGQPGGNVVLDVFVLNQRIGALLESVLAGHGIT
                     PPEYAVYSQLDVAPLTPSQLSRRLGVKASTLSGHLNALQRRGHTERVVDPQDRRSYRL
                     ELTTDGRQLLKQCQPLIRQVVQRLNRRLEADVADVRAVLAAIDEAAAQVTPS"
     gene            790..>1734
                     /locus_tag="VH419_10180"
     CDS             790..>1734
                     /locus_tag="VH419_10180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044023.1"
                     /translation="MELDRGTNNAGPFEHYPASGWVIRAAAETTRGRLSAVDAVREQV
                     GGLGRTLIDSVEGQLEDSAADAPTDAHAHADSVTQKATYAAGAVEMFAHYVDVFDRES
                     TSPRSVSALNAAYIEASSGDFGVPPTHYPKDATAQQRTSLDNERTGTISQARQATMST
                     LQAEYGRLQDVLDQNAAQVSAMLDHGPNDKDLRAMYAVGALPSYATVIYPDVNFDGVM
                     VTALPYDLRQMSDKQLAAWLVEHDEAEPTLVDVALRDTDVAHQVGLRLAHDVGDETTL
                     SSDRWDDLRRLGINQDVGAAFVYALGPHQVAKLVFGRQY"
BASE COUNT          302 a          595 c          559 g          278 t
ORIGIN      
        1 ggcggaagcg actcgctcct ggatggtggt ggtcatgacg tgcacctttc tgctctcggt
       61 tgtgctgacc ctgagcagac ttccgggata ggcgaacgac ggaatcggtc gccgaacgca
      121 agttcaggtg cgtcctgaaa cccacggaac gggcgtcgat gtacgccgga agacgccaca
      181 cacgcagtcg ttcgtacgtt cggcggcgta cgtgtgtcgt ggcccctagg gtttagggca
      241 tgcccgacca gaacgtgtcc acgtcacgac aggtcggcca gccgggcggc aacgtcgtgc
      301 tcgacgtgtt cgtgctcaac cagcggatcg gggcgctgct ggagtccgtg ctggccggtc
      361 acggcatcac acccccggag tacgccgtct actcccagct cgacgtcgcg ccgttgactc
      421 cgagccagct gagcagacgc ctcggagtga aggcttcgac gctgtcgggc cacctcaacg
      481 ccctgcagcg gcgcggccac accgagcgcg tcgtcgatcc gcaggaccgg cggtcttatc
      541 gactcgagct gacgaccgac ggtcgccagc ttctcaagca gtgtcaaccg ctgatccgcc
      601 aggtcgtgca gcgcctgaac cgtcgactcg aggccgacgt cgccgacgtc cgcgctgtgc
      661 tcgccgcgat cgacgaagcg gccgcccagg tgaccccatc gtgacctgat agcgacctgg
      721 ccgtgtatca gggtgaaccc ggtctctcct atagttcgta gccgaactag atggatcggg
      781 ggatctttca tggagctcga ccgcgggaca aacaacgcgg gtccgttcga acactacccg
      841 gcgtcgggat gggtgatccg tgccgctgcg gagacgacac gtggtcggct gtctgccgtg
      901 gacgccgtcc gcgagcaggt cggcggtctc ggccggacgc tgatcgactc ggtcgaaggg
      961 cagctcgagg acagcgccgc cgacgcaccg accgacgcac acgcccacgc ggactcggtg
     1021 acccagaagg cgacctacgc ggccggggca gtcgagatgt ttgcccacta cgtcgacgtc
     1081 ttcgaccggg aaagcacctc gcctcgctcg gtgtcggcgc tcaacgcggc gtacatcgaa
     1141 gcatcgtccg gcgacttcgg cgtcccgccg acgcactacc cgaaggacgc cactgctcag
     1201 cagcgcacgt cgctcgacaa cgaacgcacg ggcacgatct cgcaggcccg gcaggcgacg
     1261 atgtccacct tgcaggcgga gtacggccgg ctccaggacg tgctcgatca aaacgcggct
     1321 caggtctcgg cgatgctcga ccatgggccc aatgacaagg accttcgagc gatgtacgcc
     1381 gtcggcgccc tccccagcta cgcaaccgtg atctaccccg acgtcaactt cgacggcgtg
     1441 atggtcactg ccctgcccta cgacctgcgc cagatgtccg acaagcaact cgccgcctgg
     1501 cttgtcgagc acgacgaggc agagcccact ctcgtcgacg tggccttgcg ggacacggac
     1561 gtcgcccacc aggtcggcct ccgcctcgcc cacgatgtcg gcgatgagac gacgttgtcg
     1621 agcgaccggt gggacgacct tcgccgcctc ggtatcaacc aagacgtcgg tgccgcgttc
     1681 gtctacgcgc tggggccaca ccaggtggcc aagctggtgt tcggccggca gtac
//
LOCUS       DATBFZ010000330         1566 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_2847314, whole genome shotgun sequence.
ACCESSION   DATBFZ010000330 DATBFZ010000000
VERSION     DATBFZ010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2847314"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1296)
                     /gene="kdpA"
                     /locus_tag="VH419_10185"
     CDS             complement(<1..1296)
                     /gene="kdpA"
                     /locus_tag="VH419_10185"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HEY7044024.1"
                     /translation="MTVLGWLTIFGFAAILTALAMPLGRYMAAVYTGERTFLDPLFLT
                     TERLFYKLIRVNPKREQDWKAYAKSLLIFSLAGWLLLYFILRTQTLWNWTGLNPQGFH
                     SAPWNVTFNTTSSFVTNTNWQYYAGETTMSYFSQMAGLTVQNFLSAGVGIAVAVALIR
                     GIIGRSGKSIGNFWFDLIRTVLWVLTPISFVVALILVFQGSIQNFSHYVNFNGPTGLA
                     NQLAMGPVASQEAIKVIGTNGGGVFNTNSAHPFENPTRLTNLVEMLSVLIIPAALVFM
                     YGKMVGSRRQGYAIYATMMVMFLGAASVAYIAEAHGSPAQHAAGLHTQVIAGSTGGNM
                     EGKEQRFGIAGSALFDVVTTVTSCGAVNSSLESFTGIGGAVPMGNLSASEVIFGGVGT
                     GLYSILLYVLLAVFIGGLMVGRTPEWLGKKLEAREIKLAG"
     gene            complement(1296..1385)
                     /locus_tag="VH419_10190"
     CDS             complement(1296..1385)
                     /locus_tag="VH419_10190"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF021144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0043462 - regulation of ATP-dependent
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit F"
                     /protein_id="HEY7044025.1"
                     /translation="MSGADLFGLIASALVCIYLLYALLRGEKF"
BASE COUNT          317 a          481 c          500 g          268 t
ORIGIN      
        1 gccggcgagc ttgatctccc gcgcctcaag cttcttgccg agccactccg gcgtgcgtcc
       61 gaccatcagc ccaccgatga acacggccag cagtacgtac agcaggatcg agtacaggcc
      121 ggtcccgacg ccgccgaaga tcacctcgct ggccgacagg ttccccatcg gcactgcgcc
      181 gccgatgccg gtgaaggact cgagcgaact gtttaccgca ccgcacgagg tgaccgttgt
      241 caccacatcg aacagcgcgg agccggcgat tccgaagcgc tgctccttgc cttccatgtt
      301 gccgcccgtc gagcccgcaa tcacctgtgt gtgcagtccg gccgcgtgct gggctgggga
      361 cccgtgcgct tcggcgatgt acgcgacgct cgcggcgccg aggaacatca ccatcatcgt
      421 ggcgtagatg gcgtaaccct gccggcggct gccaaccatc ttgccgtaca tgaacacgag
      481 cgcggcgggg attatcagca ccgaaagcat ctcgaccagg ttggtcaacc tggtcgggtt
      541 ctcgaacggg tgcgcggagt tcgtgttgaa gacgccaccg ccgttcgtac cgattacctt
      601 gatcgcctcc tgggaagcga caggacccat cgcgagttgg ttggccaggc cggtcggacc
      661 gttgaagttc acgtagtgag agaagttctg gatcgagccc tggaagacca ggatcagcgc
      721 gacgacgaac gagatcggcg tcaataccca cagcacggtg cggatcaggt cgaaccagaa
      781 gttcccgatg ctcttgccgc tgcgtccgat gatcccgcgg atcagcgcca ccgcgactgc
      841 gatccccact ccggcagaca ggaagttctg cacggtgagc ccggccatct ggctgaagta
      901 gctcatcgtg gtctcgccgg cgtagtactg ccagttcgtg ttggtcacga acgacgaggt
      961 ggtgttgaac gtgacgttcc atggagctga gtggaacccc tgtgggttca atccggtcca
     1021 gttccagagc gtctgggtgc gcaggatgaa gtagaggagc agccagccgg ccagcgagaa
     1081 gatcagcagg ctcttggcgt acgccttcca gtcctgctcg cgcttcgggt tgacccggat
     1141 gagcttgtag aacaggcgct ccgtggtcag gaagagcgga tcgaggaacg tccgctcgcc
     1201 ggtgtagacg gccgccatgt agcggccgag cggcatcgcc agagcagtga ggatggcggc
     1261 gaagccgaag atggtcagcc agccgaggac tgtcattaga acttctctcc gcgcagcagc
     1321 gcgtagagca ggtagatgca gaccagggct gaggcgatca gcccgaacag gtcagcgccg
     1381 ctcatatccg gtcgatcccg tacacgagac cgagcagcac ggcgaacatc accagcccga
     1441 tcaatatggc tacaacgtcc atggacagat cgtggcccgt gtggaatcaa gagaccgtgc
     1501 agaaagcacg cgcgggcata cagatgttgt gaaggacagc ctttacagct gcaggccgtc
     1561 ggcgat
//
LOCUS       DATBFZ010000331         8577 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_2195998, whole genome shotgun sequence.
ACCESSION   DATBFZ010000331 DATBFZ010000000
VERSION     DATBFZ010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 8577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8577
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2195998"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..903)
                     /locus_tag="VH419_10195"
     CDS             complement(<1..903)
                     /locus_tag="VH419_10195"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I subunit 5 family protein"
                     /protein_id="HEY7044026.1"
                     /translation="MSGLDESAPVVLVALPLLAAAVIAGVGHWIPRIAADSTAVGCAA
                     VCCVVSAWLTLDTGSVRRVVWLGDYVPEHGHSVGIALVADRAGCALAFLAAGLTLAGA
                     VYSWHFLEDVDASDHALVLVFLAGMSGFALAGDLFNAFVWFELMSVAAFALTGLRVEE
                     PRSVQGALTFGAMTSWAATVSLLGIALLYAVTGELNLAAVGQDLRTDGGSRTVDVACA
                     LLFTGFLVKAAAVPWHFWTADAEAVAPTPVCALLSGAMVALGVYGVARSWWTMFDGVV
                     TTAAIRSALLVLGVLTALVGALMCT"
     gene            complement(900..1250)
                     /locus_tag="VH419_10200"
     CDS             complement(900..1250)
                     /locus_tag="VH419_10200"
                     /inference="COORDINATES: protein motif:HMM:NF012636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter subunit C"
                     /protein_id="HEY7044027.1"
                     /translation="MLPYLVAGWIFAVACYGLVTSRNLVHAIACLSIAQSSTYVLLLA
                     IGYTTGGTAPVFSDIQPGAKVVDPVVQALVLTDIVVGATVTALLLAIAVQVAKQHGTV
                     DPEEILSLEEESAQ"
     gene            complement(1256..2029)
                     /locus_tag="VH419_10205"
     CDS             complement(1256..2029)
                     /locus_tag="VH419_10205"
                     /inference="COORDINATES: protein motif:HMM:NF015970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MnhB domain-containing protein"
                     /protein_id="HEY7044028.1"
                     /translation="MSSRHRPTTMAVFWVGAAGFAALFALAAWGLPSVGDVAHPYAER
                     AVPTAVQHDTANVIGSVTFDQRGLDTLGEEFILLAAAVGTILLLRRLSGEDEESGVEH
                     EFRQRDVFDAVRLAGVALLPVTLLVGAYIVLHGTVSPGGGFQGGVVLATGVHLAYLGG
                     DYHVLERLRPVLVFDVGEAIGAAGYVIVGAVGVVVTGSFLANVLPTGTMGDVNSGGTV
                     FVLNVAVGFEVGSALVLLIARFLDQGLLIRAGGSTGQRS"
     gene            complement(2026..2322)
                     /locus_tag="VH419_10210"
     CDS             complement(2026..2322)
                     /locus_tag="VH419_10210"
                     /inference="COORDINATES: protein motif:HMM:NF024641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4040 domain-containing protein"
                     /protein_id="HEY7044029.1"
                     /translation="MSPELQQVMLLVVLAVVALTATAAVLTRVPQRQAIVLSAYGLVM
                     GLLMVVFHAPDVAMSQLAIGSAAVPLLIVLAITACDRESRARGDTRKRQQREDR"
     gene            complement(2319..2627)
                     /locus_tag="VH419_10215"
     CDS             complement(2319..2627)
                     /locus_tag="VH419_10215"
                     /inference="COORDINATES: protein motif:HMM:NF015302.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HEY7044030.1"
                     /translation="MSATGLLGALLLWSGIGVMVAMAIGVLVAGRVLSRLHAASAVAT
                     PGGLLVCLGLAVRADTWHDVAKLLIIAVLLVMSSAVGASVTGRAAVKTEAAAGERSQQ
                     "
     gene            complement(2624..2869)
                     /locus_tag="VH419_10220"
     CDS             complement(2624..2869)
                     /locus_tag="VH419_10220"
                     /inference="COORDINATES: protein motif:HMM:NF015993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter complex subunit
                     F"
                     /protein_id="HEY7044031.1"
                     /translation="MSVAFVVVVAGAVLPCVLATMRGGPLLRLAALQALGVAVSVAVL
                     LFAAAVDRVAYVDAALVVALLSLAGSLVFARFFGHVL"
     gene            complement(2866..3336)
                     /locus_tag="VH419_10225"
     CDS             complement(2866..3336)
                     /locus_tag="VH419_10225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044032.1"
                     /translation="MSFVVWWLVLLGLYVALISTLSIAELVVGALVAAIAAAVALLTA
                     RAFDVDATLPAFRWRWLLSFPVDAGIDVARLTRRVVSCLPGRRTTAGWWDTAELPDDA
                     GPTEAVRAYAVLVVSLTPASYVVDVHVDDESDDAGVLQIHRWAKKGPVERAVTP"
     gene            3646..5133
                     /locus_tag="VH419_10230"
     CDS             3646..5133
                     /locus_tag="VH419_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY7044033.1"
                     /translation="MPEEPRLPDPALVVLVGASASGKSTWAQARFRSAEIVSSDDLRA
                     VIGSGRHDLDATDEAFSLLDQIVAGRLRRGLTTVVDTLGLDAVRRRGQRDLGKAAALP
                     TVAVLFDTPAELCRRRNAARDRPVPARVLTDQLKRFRPVLDEVAAEGWDLVTTVAGDE
                     ETGPAQSRAAAARVLLPELPTVPDRSGKGLDVVLQVSRFPWGEDPSGWLKGIALAADE
                     AGFAGLALMDHLIQIPQVGREWEAIPEPWVVLGMLAGLDTRLRLGTLVTPVTFRPPGI
                     VAKTVATLDVISGGRAFCGLGAGWWEREHAAFGLPFPGPAERLDRLETAIETMRALWA
                     AGTKPYAGERVSLPETTSYPRPVSDVPIIVGGGGERRTLRIAARLADGCNLTSEPEPF
                     ARKKAALLVHCRDVGRDPDTLDVTVLDLPVVGNDRDDAWDRLERLRGRRAAAAYARRH
                     VPGTPADHRQRYQRMAEAGVSTVFVAFADLVGPDDLLARAGLVRS"
     gene            complement(5128..5952)
                     /locus_tag="VH419_10235"
     CDS             complement(5128..5952)
                     /locus_tag="VH419_10235"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEY7044034.1"
                     /translation="MGRLWRIARELVLLAALYVAYSLARLLASDDTREAIKHARDVVG
                     IERWWHIGVEVSWNSALSQHEAFSVFAGYWYASMHYVVTPIVLVLLWRHSHAHYLKMR
                     RVLVGATAIALLAYIFYPTAPPRMMHGYVDVLSTTSHWGWWGASASAPRGLGDLTNQL
                     AAMPSMHVGWALWCTLGIMSITRTLWMRIGAVLYVVVTTLVVVATANHWILDAIIGAA
                     ITAGVWFLVRQHAKMREAKRRDTVDGPGSVAAPVLARSELDELAQPSEPELVSVRS"
     gene            6280..7005
                     /locus_tag="VH419_10240"
     CDS             6280..7005
                     /locus_tag="VH419_10240"
                     /inference="COORDINATES: protein motif:HMM:NF024948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HEY7044035.1"
                     /translation="MRRDPLWLSGIAGAAIVLAAWATTTTFVPALRGAVPPGNHGAHQ
                     AAQVHDRAHPPNQTPPDRDPGSLPLAFEIAFYVLLVLFAAGLLLIVVHRVRWRRLRRR
                     TSTFETDIDAVADDIDAQLPELLRRSATQSLAELAEGSPRNAIVRCWLQLEDAVVSLG
                     LRRDPASTSEEFTATVLARFAVGRRSIDELGVLYREARFSEHDLDETHRRRAVRALMA
                     LRDDLDVAAAEAAAPRRPSGIDA"
     gene            7007..7504
                     /locus_tag="VH419_10245"
     CDS             7007..7504
                     /locus_tag="VH419_10245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044036.1"
                     /translation="MTRVVSSAWIKRGLYALGTGLVASGFTAWLGLRPRPVLVVLVTV
                     SCFAVGWLLADLAARVTPVDWVAPRHRSVPRRGLDPRFSRLARKLRDDVGWEIAARDL
                     HTTLVSIVDERLAAHHGIDRRQQPAAAQAVLGEELSGYIEEAPRVRRGQAAYVSKLLT
                     RIEAL"
     gene            7501..8511
                     /locus_tag="VH419_10250"
     CDS             7501..8511
                     /locus_tag="VH419_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012919084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEY7044037.1"
                     /translation="MTPAQTTGTERGQSPEPLGLAAVSARAAAVLDEVEVAVVGKRSA
                     LELVLSAVLAGGHVLLEDFPGLGKTLAARSFAQTLGLEFTRAQFTPDLLPGDLTGSFI
                     YDQRAGEFAFRKGPLFTGLLLADEINRTPPKTQAALLEAMQEHQVTVEGETFVLPAPF
                     HVLATANPVEYEGTYPLPEAQLDRFLMRVSFGYPSADEETEILRRRMARQREEQTLRP
                     ITDPPTLLTMQAAVETVTIDDSVSRYCVDLAAATRTHAHILIGSSPRGALGLLLGGRA
                     LAVVRGRDYVVPEDVKAVAHPVLAHRLTLKPELWMTQITSHTVVDAVLGSVPAPSARE
                     RV"
BASE COUNT         1464 a         3070 c         2871 g         1172 t
ORIGIN      
        1 ggtgcacatc agtgctccga cgagcgcggt gagcacgccg aggacgagca gcgcggagcg
       61 gatcgccgct gtcgtgacca cgccgtcgaa catcgtccac caggagcgag cgacgccgta
      121 cacccccagc gccaccatcg caccggacaa cagcgcgcag accggggtcg gcgcgaccgc
      181 ttccgcgtcg gccgtccaga agtgccacgg caccgccgcc gccttgacga ggaacccggt
      241 gaacagcagc gcacaggcca cgtccacggt ccgggagccg ccgtccgttc gcaggtcttg
      301 gccgacggcg gccaggttga gttcgccggt gacggcgtac agcagcgcga tcccgagcag
      361 ggacacggtc gccgcccacg acgtcatcgc gccgaacgtc agcgcgccct gcacgctgcg
      421 tggctcctcc acgcgcaacc cggtgagcgc gaacgcggcc acgctcatca gctcgaacca
      481 aacgaaggcg ttgaagaggt cgccggcgag ggcgaagccc gacataccgg caaggaacac
      541 cagcacgagc gcgtgatcac tggcgtcgac atcctcgagg aagtgccagg agtagacggc
      601 tccggcgagg gtgagccccg cagcgaggaa cgccagggcg cagccggccc ggtcagcgac
      661 caatgcgata ccgaccgagt gcccgtgctc aggtacgtag tcccccagcc agaccactcg
      721 acgaaccgag ccggtgtcga gtgtcagcca agcggagacg acgcaacaca cggcggcgca
      781 gccgaccgcc gtcgagtctg ccgcgatccg ggggatccag tggccgacgc ccgcaatgac
      841 cgcagccgcc aggaggggaa gagcgaccag gacgaccggg gcgctctcgt cgagcccgct
      901 cactgcgcgg actcctcctc caacgacagg atctcctcgg ggtcgacggt cccgtgctgc
      961 ttcgcgacct ggaccgcgat ggccagcagc agtgcggtca ccgtggcacc cacgacgatg
     1021 tcggtcagca ccagagcctg tacgaccggg tcgaccacct ttgccccagg ctggatgtcg
     1081 gagaagaccg gcgcggtgcc gccggtcgtg tagccgatcg ccagcaacag cacgtacgtc
     1141 gacgactggg cgatggacag gcacgcgatc gcgtgcacga ggttccggct cgtcaccaag
     1201 ccgtagcacg cgacggcgaa gatccagccg gcgaccaggt acggcaacac gcccgtcacg
     1261 atcgttgccc cgtcgagccg cccgcccgga tgagcagccc ctgatcgagg aaccgtgcga
     1321 tcagcagcac gagcgcggaa ccgacctcga acccgacggc aacgttcaac acgaagacgg
     1381 ttccgcccga gttcacgtcg cccatcgtcc cggtcggaag gacattggcc aggaacgagc
     1441 cggtcaccac gacaccgacc gcgccgacga tcacgtagcc ggcagccccg atcgcctcgc
     1501 ccacgtcgaa gacgagcacc ggacgcagcc gctccagcac gtggtagtcg ccccccagat
     1561 aggccagatg gacgccggtg gccagcacca cgccaccctg gaagccgcca cctgggctga
     1621 ccgtgccgtg gagcacgatg taggcgccga cgaggagggt gaccgggagc agcgccacgc
     1681 cggcgagccg gacggcgtcg aacacgtccc gctggcggaa ctcgtgctcg acgccggact
     1741 cctcgtcctc accgctgagc cgccgaagca gcaggatcgt gcccaccgcg gcggcaagca
     1801 ggatgaactc ctcaccgagc gtgtcgaggc cgcgctggtc gaaggtcacc gagccgatca
     1861 cgttggcggt gtcgtgctgc accgccgtcg gcaccgctcg ctcggcgtac gggtgcgcga
     1921 cgtcgccgac cgacggcaga ccccacgccg ccagcgcgaa gagcgccgcg aacccggcag
     1981 cgccaaccca gaacaccgcc atggtcgtcg ggcggtgccg gctgctcatc ggtcctcccg
     2041 ctgctgtcgc ttccgggtgt ctccgcgggc gcggctctcc cggtcacagg cggtgatcgc
     2101 gagcacgatc agcaacggca cggccgccga gccgatcgcg agttgggaca tggccacgtc
     2161 gggcgcatgg aagaccacca tcagcaaccc catcaccagg ccgtacgccg acagcacgat
     2221 cgcctgacgc tgcggtaccc gagtgaggac ggcggctgtt gcggtgagtg cgacgaccgc
     2281 caagaccacg agcagcatca cctgctggag ctcaggactc attgctgact cctttcaccg
     2341 gccgccgcct ctgtcttgac cgcagcccgt ccagtcaccg acgcaccgac cgcgctgctc
     2401 atcaccagga gcacggcgat gatcagcagt ttggcgacgt catgccacgt gtcggcgcgg
     2461 accgcgaggc cgaggcagac cagcagaccg cccggggtgg ccaccgcact cgccgcatgc
     2521 agccgactca gtacgcgtcc agcaacgagc actccaatgg ccatcgccac catcacgccg
     2581 ataccgctcc acaggagcag cgcaccgagg agaccggtag cgctcacagc acatgtccga
     2641 agaagcgagc gaacaccagt gatccggcca gtgacaacaa cgcaacgacg agcgccgcgt
     2701 ccacgtaggc cacccggtcg acggccgccg cgaacagcag cacggcgaca ctgacggcga
     2761 cccccaacgc ttgcagtgcc gccagtctga gcagcgggcc accgcgcatc gtcgccagca
     2821 cacaaggaag cacagcgcct gccacgacca ccacgaacgc gacgctcacg gcgtcacggc
     2881 ccgttcgacg gggcccttct tggcccagcg atggatctgg agcacgccgg cgtcgtcgga
     2941 ctcatcgtcg acgtgcacgt ccacgacgta cgaggcgggc gtcaacgaga ccaccagcac
     3001 ggcgtacgcc cggaccgcct ctgtcggccc ggcgtcgtcc gggagttccg cggtgtccca
     3061 ccaaccggcg gtcgtgcgcc ggcccggaag acagctgacg acacgacgcg tcaatcgggc
     3121 aacgtcgatg ccggcgtcga ccgggaagga caacagccaa cgccaccgga acgccggcag
     3181 cgtcgcgtcc acgtcgaagg cgcgcgcggt cagcagcgcg accgcggcgg cgatggcggc
     3241 gaccagtgcc cccaccacca gctcagcaat ggaaagcgtg ctgatcagcg cgacgtacag
     3301 gccgaggagg accagccacc agaccacgaa tgacatcagc cgacgcgggc ccatcgctca
     3361 accactccct ctcttgcgca cccgcctccg taccctggcc gccgctctcc atgccgacat
     3421 cccggttctc ctgacggtgg tggtttgcga tgtttacagc ccaagcggcg tgtcaacatc
     3481 gcagacctcc gatgggcaca gtgggtctcg gatgtggaca tcgcactccg cgggcggccg
     3541 agctccgaat ccgaccgtca caccccgggt atcgcgacga gcccgctcac acacccctct
     3601 cgagctccag gatccggccc gcccgagccg tgtcagactc ctggcatgcc ggaagagccg
     3661 cgtctgccgg accctgcgct cgtcgtgctg gtcggggcgt cggcatctgg caagtcgacg
     3721 tgggcgcagg ctcgtttccg aagcgccgag atcgtctcgt ccgacgacct gcgcgcggtc
     3781 atcggcagtg gccgacacga cctggacgcc accgacgagg cgttcagctt gctggaccag
     3841 atcgtggcgg gacgcctccg gcgcggtctc accacggtcg tcgacacgct cgggctggac
     3901 gccgtacgac ggagggggca acgcgacctg ggaaaggcgg cggctctgcc gacggtcgcg
     3961 gtccttttcg acacgcccgc cgagctgtgt cgccggcgca acgcggcgcg cgacagaccg
     4021 gtaccggcgc gagtcctgac cgaccagctc aaacggttcc gtcccgtcct cgacgaggtg
     4081 gcagccgaag gctgggacct cgtcacgacg gtcgccggcg acgaagagac cggcccggct
     4141 cagtcgaggg ctgccgcggc ccgcgtcctc ctgcccgagt tgccgacggt gcctgatcgt
     4201 tcagggaagg ggctcgacgt cgtgctccag gtgtcgcgct tcccttgggg cgaggacccg
     4261 tccggatggc tcaagggcat cgcactcgcg gcggacgagg cagggttcgc tggtctcgcg
     4321 ctgatggacc acctgatcca gatcccgcag gtcggccgcg agtgggaggc catcccggag
     4381 ccctgggtcg tgctcgggat gctggccggt ctcgacaccc ggctacggct cggcacgctg
     4441 gtgacgccgg tgacgttccg accgccaggc atcgtcgcaa agacggtcgc caccctggac
     4501 gtcatctcag gagggcgagc cttctgtggc ctcggcgccg gctggtggga gcgcgagcat
     4561 gcggcgttcg gcctgccgtt ccctggcccg gcagagcggc tcgaccggct ggagacggcg
     4621 atcgagacga tgcgggcgct gtgggcggcc gggacgaagc cgtacgccgg ggaacgcgtg
     4681 agccttcccg agaccacctc gtacccgcgg ccggtctccg acgtgccgat catcgtcggt
     4741 ggcggcggtg agcgacggac cctccgcatc gccgcccgac tggctgacgg ctgcaacctg
     4801 acgtccgagc ccgagccgtt cgcccgcaag aaggcagcgc tgctcgtcca ctgccgtgac
     4861 gtgggccgcg accctgacac gctcgacgtg accgtgctcg acctgcccgt ggtcggcaac
     4921 gatcgtgacg atgcgtggga ccgactcgag cggctgcgcg gccggcgtgc cgctgcggca
     4981 tacgcgagaa ggcacgtccc aggtacccct gcggatcatc ggcagcgcta tcagcggatg
     5041 gccgaggcag gcgtcagcac ggtcttcgtg gcgttcgccg acctcgtcgg cccggatgac
     5101 ctcctcgcga gggccgggct ggtcaggtca tgaccgaacg gagaccagct ccggctcgga
     5161 gggctgcgcc aactcgtcga gctcgctgcg cgccaacact ggagcagcaa cggaaccagg
     5221 tccatcgacc gtgtcccgcc gtttggcctc ccgcatcttg gcgtgctgac ggaccaggaa
     5281 ccacacgcct gcggtgatcg cggcgccgat gatcgcatcg agaatccagt ggttggcggt
     5341 cgcgaccaca accagagtcg tcacgacgac gtacagcact gcaccgatac gcatccacag
     5401 tgtgcgggtg atcgacatga tgcccaacgt gcaccacaac gcccaaccga cgtgcatcga
     5461 cggcattgcg gcgagctggt tggtcaggtc accgagaccg cgtggtgcgg acgcagaggc
     5521 gccccaccag ccccagtgcg acgtggtgga cagcacgtcg acgtagccgt gcatcatccg
     5581 cggcggagcc gtcgggtaga agatgtacgc gagcaacgcg atggcggtgg ccccaaccag
     5641 cacccgtcgc atcttgagat agtgcgcgtg tgaatggcgc cacagcaaga cgagcacgat
     5701 gggtgtcacg acgtagtgca tgctggcgta ccagtagccg gcgaacacgc tgaacgcctc
     5761 gtgctgggac aacgcggagt tccaggacac ctcgacgccg atgtgccacc agcgctcgat
     5821 gccgaccacg tccctggcgt gcttgatcgc ctcgcgggtg tcgtcagagg ccagcagccg
     5881 ggccagcgag taggcgacgt agagggcagc gagcagcacg agctcgcgag caatgcgcca
     5941 cagccgtccc atcacgcccc cttcccgtgc accctgactg cactctacaa gtgggtggca
     6001 acagatttct gcagccgggt attccgcctc ggcgggagtc acgcgctgat cgggcacgcc
     6061 gtccaggatc accgcgcggc gccgttgata cctccgggag ccccccgatt gcgcccccgg
     6121 aggtccccac gtgcatcagg gtcatcactc tccgtcatcg taggacagca tgacgtgaca
     6181 ttcccacccg cctccctggc atgcgccgcc ggatgacctg acgatcaggc gacacgccga
     6241 gcagacctgg cgccatgggg cattgactac agtcacgtca tgaggcggga cccgctgtgg
     6301 ctgtccggga tcgccggtgc agcgatcgtg ctcgccgcct gggccaccac cacgaccttc
     6361 gtccctgccc tccgcggcgc tgtcccaccg gggaaccacg gcgcgcacca ggcggctcag
     6421 gtgcacgacc gcgcgcaccc acccaaccag acacctcccg accgcgaccc cggcagcctg
     6481 ccgctggcct tcgagatcgc gttctacgtc ctgttggtcc tgtttgcggc cgggttgctg
     6541 ctcatcgtgg tgcaccgggt gcgctggcgg cggctgcggc gcaggacgtc cacgttcgag
     6601 actgacatcg acgccgtcgc cgacgacatc gatgcacagc tcccggagct gttgcggcgg
     6661 tctgccacgc agagtcttgc cgagctcgcg gaaggttcac cgcggaatgc gatcgtccgc
     6721 tgctggctcc agctcgagga cgccgtcgtc tcgctcggtc tccgccgcga ccctgcctcg
     6781 acctcggagg agttcaccgc gaccgtgctc gcacggttcg cggtgggccg acgcagcatc
     6841 gatgagctcg gcgtgctcta ccgcgaggcg cgcttctccg aacatgacct ggacgagacg
     6901 caccgcagac gggccgtgcg cgccttgatg gcgttgcgcg acgacctgga cgtcgccgcc
     6961 gccgaagccg ccgccccacg acggccgagc gggatcgacg cgtgacatga cgagagtggt
     7021 gtcgtcggca tggatcaagc gagggctgta cgcgctgggc accgggctgg tggcgtcggg
     7081 cttcaccgca tggctcggtt tgcggccgag accggtgctc gtcgtcctgg tcacggtctc
     7141 ctgcttcgcc gtcggctggc tgttggcaga cctcgccgcg cgcgtcaccc ccgtcgactg
     7201 ggttgcaccg cgccatcgaa gtgtgccgcg ccgaggcctg gacccacggt tttctcggct
     7261 ggcgcgcaag ctgcgcgatg acgtcggctg ggagatcgcc gcacgagacc tgcacaccac
     7321 gctcgtgagc atcgtcgacg agcggctcgc cgcacatcat gggatcgacc ggcggcagca
     7381 gccggcagcc gcccaggctg tcctcggcga ggagctgtcc ggttacatcg aggaggcgcc
     7441 gcgggtacga cgaggccagg cggcgtacgt ctcgaaactg ctgacccgaa tcgaggcgct
     7501 gtgacaccgg cacagaccac cggaaccgag cgcggccaga gtccagaacc actcggcctc
     7561 gcagcagtca gtgcgcgcgc ggccgccgtc ctcgacgagg tcgaggtggc ggtggtcggg
     7621 aagaggtccg cgctcgagct cgtcctgagc gcggtcctcg ccggcggcca cgtgctgctc
     7681 gaggacttcc ctggactggg caagacgctc gccgcccggt cgttcgcaca gacgttggga
     7741 ctggagttca cccgtgcaca gttcaccccg gacctcctgc caggcgacct gaccgggtca
     7801 ttcatctacg accagcgggc aggcgagttc gcgttccgga aaggaccgct gttcaccggc
     7861 ctgctcctcg ccgacgagat caaccgcacg ccgccgaaga cgcaggccgc gctgctggag
     7921 gccatgcagg agcaccaggt gacggtggag ggtgagacgt tcgtgctgcc ggcacccttc
     7981 cacgtgctgg ccacggccaa cccggtcgag tacgaaggca cctatccctt gcccgaagca
     8041 cagctcgacc gcttcctgat gagggtcagc ttcggctatc cgagtgcgga cgaggagacc
     8101 gagatcctcc gacgccggat ggctcgacag cgtgaggagc agaccctgcg gccgatcacc
     8161 gacccaccca cgctgctgac gatgcaagcg gccgtcgaga ccgtcactat cgacgacagc
     8221 gtgagtcgct actgcgtcga cctggcggca gcgacccgga cgcacgcgca catcctcatc
     8281 gggtcttcgc cgcggggcgc gctcggcttg ctgctcgggg gtcgggccct ggcggtggtc
     8341 cgtgggcgtg actacgtcgt cccggaagac gtcaaggccg tggcacaccc ggtgctggcc
     8401 catcggctga cgctcaagcc ggagctgtgg atgacacaga tcaccagtca cacggtcgtc
     8461 gatgcggtcc tgggttcagt ccctgcgccg tcggcacggg agcgagtctg accatggcct
     8521 ggcggccgac gtgggcgcac gtccgggcgc tgctgctcgg cggcgtcggc gtgctcg
//
LOCUS       DATBFZ010000332         3799 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_3178828, whole genome shotgun sequence.
ACCESSION   DATBFZ010000332 DATBFZ010000000
VERSION     DATBFZ010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 3799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3799
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3178828"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1187
                     /locus_tag="VH419_10255"
     CDS             <1..1187
                     /locus_tag="VH419_10255"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEY7044038.1"
                     /translation="QDDPNRGIGAARDWIAAEMTKYAAASGGRMTVTVPSYIQQPGPN
                     DPRIDVPTRISNVVATLRGSVDPNRTYVITGHYDSRVTDVMNRTADAPGADDDASGVA
                     VSMELSRVMASHRPDCTLIFAAVAGEEQNLYGSTFLAQQLADAGANVQGMFTNDIVGS
                     SRADDGTRDPFSLRLFAQGPPDTEDDATHARRLSIGGENDSPARELARFVAEVAGQQT
                     TKMNIRMIYRLDRFLRGGDNIPFLEHGWPACRFTEPNENFAHQHQDVRVENGVQFGDL
                     PEFVDFDFVGRVARVNAATLWSLSQAPAMPTNVKVDTSVLTNDTRLVWTQGNDANLAG
                     YEVVWRPTINPFWTHVIPVGNVSNATVNLSKDNVIFGVRAVSTDGQHSPAVFPFPLPP
                     PT"
     gene            complement(1194..1703)
                     /locus_tag="VH419_10260"
     CDS             complement(1194..1703)
                     /locus_tag="VH419_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020385408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEY7044039.1"
                     /translation="MPERIEPRSLPADERESLESWLDFHRATLLMKCDGLTHEQLTMA
                     SCPPSTLSLIGLVRHLTGVEGWFHEFDDEPDVDVFDDEDPFSPRPDLVERDFSTYRIS
                     VDRARRAAAAHRLDDVMDLPHWVADDRPKELRPTSLRWVYQHMIEEYARHNGHADLVR
                     ERIDGAVGD"
     gene            1978..3249
                     /locus_tag="VH419_10265"
     CDS             1978..3249
                     /locus_tag="VH419_10265"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HEY7044040.1"
                     /translation="MVEVLVRPADEVDVRLIEAEVAEVAGTLNAAHGRLVELAARLIE
                     TEAWRGHGIKSVEHWLSWHAGLSHGRAAAVTAVARRRADLPTTVESLARGELSVEQVR
                     EIARFVPGHNDAEAAALARVSTVSQLRNTLARYRFEPDAGESGDAAEPAPDPAARRGT
                     ASMTHDGLRFGLHVDAPLDDGAIIEQALTEAKDALFGSGHAEVTWMDAVVEVCRRSLD
                     TVTSEGRSSRYRVYVHLDTDGGWLNAGPALPDSLMGKICCDGVVVPVWETTGAPVNVG
                     RSQRIVPDRTRRLVTDRDRGCVVPGCGSRYHLEVHHLVAWQRGGRTDTDNLACLCPFH
                     HDALHRGELCINGNADRPETLSFVDGLGREMPGLVPPLLPQVPPRLDVVRAARPRYQR
                     PPGERIDNRCVHFTPKSWDRLSVAGPNAPPT"
     gene            3511..>3799
                     /locus_tag="VH419_10270"
     CDS             3511..>3799
                     /locus_tag="VH419_10270"
                     /inference="COORDINATES: protein motif:HMM:NF017999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase subunit G"
                     /protein_id="HEY7044041.1"
                     /translation="MLVSGSATVGAAPDEVWKALNDPAVLVRTIPGCQRLEEIGPDSY
                     TMTVTAGVASIKGVYTGQVRLTDQQPPDTFVLKASGAGGPGTVSADVRVTLR"
BASE COUNT          615 a         1290 c         1301 g          593 t
ORIGIN      
        1 gccaggacga cccgaaccgg gggatcggtg ccgcccgcga ctggatcgcc gcggagatga
       61 ccaagtacgc cgctgcgtca ggcggccgga tgaccgtgac cgtgccgtcg tacatccagc
      121 agccggggcc gaacgatccc cgcatcgacg tgccgacccg gatcagcaac gtcgtggcga
      181 cgctcagagg cagcgtcgac ccgaaccgga cctacgtgat caccggccac tacgactcgc
      241 gggtgaccga cgtgatgaac cggaccgcgg acgcccccgg cgccgacgac gacgcctcgg
      301 gggtcgcggt gagcatggag ctgtcccgcg tgatggcgtc gcaccgaccg gactgcaccc
      361 tgatcttcgc ggcagtcgcc ggggaggagc agaacctcta cgggtcgacg ttcctcgcgc
      421 agcagctcgc cgacgcggga gcgaacgtcc agggcatgtt caccaacgac atcgtcggca
      481 gcagccgcgc cgacgacgga acccgtgacc cgttcagcct gcggttgttc gcccaggggc
      541 cgccggacac cgaagacgac gcgacgcacg ctcggcggct gtcgatcggc ggcgagaacg
      601 actcgcccgc gcgtgagctg gctcgtttcg tcgccgaagt cgcgggacag cagaccacga
      661 agatgaacat ccggatgatc taccgtctgg accggttcct gcgcggtggt gacaacatcc
      721 cgttcctcga gcacggctgg cctgcctgcc ggttcaccga gccgaacgag aacttcgcgc
      781 accagcacca ggatgtgcgg gtggagaacg gcgtccagtt cggcgacctc ccggagttcg
      841 tcgacttcga cttcgtcggt cgggtcgccc gggtcaatgc cgccaccctg tggtcgctct
      901 cccaggcccc ggccatgccg acgaacgtca aggtggacac gtccgtgctc accaacgaca
      961 cccggttggt ctggacccag ggcaacgacg cgaacctggc gggatacgag gtcgtatggc
     1021 gcccgacgat caacccgttc tggacgcacg tgatcccggt cggcaacgtg tcgaacgcga
     1081 ccgtgaacct gtcgaaggac aacgtcatct tcggcgtacg ggcggtgagc accgacgggc
     1141 agcacagccc cgccgtgttc ccgttcccgc tcccaccccc gacctgaccc cggtcagtcc
     1201 ccgacggcgc catcgatgcg ttcccggacc aggtcggcgt gaccgttgtg ccgggcgtac
     1261 tcctcgatca tgtgctggta cacccagcgc agggaggtgg gtcgcagctc cttgggacgg
     1321 tcgtcggcca cccagtgcgg caggtccatg acgtcgtcga gacggtgggc agcggcggca
     1381 cgtcgtgccc gatcgacact gatccgatag gtgctgaagt cacgttcgac gaggtccggt
     1441 ctcggcgaga acgggtcctc gtcgtcgaac acgtccacgt cgggctcgtc gtcgaactcg
     1501 tgaaaccagc cctcgacacc ggtcaggtgg cggaccagac cgatcaggct gagcgtcgac
     1561 ggcgggcagg acgccatcgt gagctgctcg tgagtcagcc cgtcgcactt catcaggagc
     1621 gttgctcggt ggaagtccag ccagctctcg agcgactcgc gctcgtcggc aggcaggctc
     1681 cgcggctcga tccgctccgg catgactccc cctcgctcgg gctgcacccg ggctcgctcg
     1741 tggtacgacg gcagctgtgg tcctcgtgtc gaccccgtga tcgtaggtgc tccgtagagc
     1801 gggtcggcgt gccgccgcca gcgagcccct tcggcctgct gctccggtca gagcgatcga
     1861 aaacgcgtgg acggcggccg gaccgctggt ttgagagcgc aagaaggagc agtcaggtgt
     1921 ccacagatcg cggttgaccc atcgtgcagg tcttcgaaca ggtgtacgat tagtgtcatg
     1981 gtggaggtgc tcgtacgacc cgcggacgag gtcgacgtgc gcctgatcga ggccgaggtc
     2041 gccgaggtgg ccggcacgct caatgcagca cacggtcgac tcgtcgagct cgcggcccgg
     2101 ctgatcgaga cggaggcctg gcgcggccac gggatcaagt cggtcgagca ctggctgtcc
     2161 tggcacgccg gactgtcgca cgggcgagcg gcggcggtca cagccgtggc tcggcgacgg
     2221 gccgacctcc cgacgacggt cgagtcgttg gcccggggtg agctctccgt cgagcaggtc
     2281 cgtgagatcg ccaggttcgt gccgggccac aacgacgccg aggcagccgc cctcgctcgc
     2341 gtgtcgaccg tgtcacagct gcgcaacacc ttggcgcgat accgattcga gcccgacgcc
     2401 ggtgagtctg gggacgccgc cgagccagca cccgatccgg ctgcacggcg cggaaccgcg
     2461 tcgatgactc atgacggcct ccggttcggg ctgcacgtgg atgctccgct ggacgacggc
     2521 gcgatcatcg agcaggcgct caccgaggcg aaggacgcgc tgttcgggtc cggtcacgcc
     2581 gaggtcacct ggatggacgc cgtggtcgag gtatgccgtc gatcactcga cacggtgacg
     2641 tccgagggcc ggagctcgcg ctatcgggtc tacgtccacc tggacaccga cggtggctgg
     2701 ctcaatgcgg gtcccgctct gcctgacagc ttgatgggca agatctgctg cgacggagtc
     2761 gtggtgccgg tctgggagac aacgggtgca ccggtcaacg tcggaagatc ccagcggatc
     2821 gtccccgacc gcaccaggcg gctggtcacc gatcgcgatc gcggctgcgt cgtgcccggc
     2881 tgcgggtctc gctaccacct cgaggtgcac catctcgtgg cctggcagcg aggtggtcgt
     2941 accgacaccg acaacctcgc ctgcttgtgc ccctttcacc acgacgcact gcaccgtggc
     3001 gagctatgca tcaacggaaa tgccgatcga ccggagacct tgagcttcgt cgatgggctt
     3061 ggacgcgaga tgccaggtct ggttccgccg cttctgccgc aggttcctcc gcggctcgac
     3121 gtggtcagag ccgcccgtcc gcggtaccaa cggccacccg gtgagcggat cgacaaccgc
     3181 tgcgtccact tcaccccgaa gagctgggat cgcctcagcg tggccggtcc gaacgcgcct
     3241 cccacctagg cgacgacggg tgacggcggc gaacgggatc gctgtcagtt cgtcacgttg
     3301 agcgtggcag cggtcgccac ccgtcgggag ctcctagtac gtatcgcccc aggatggggc
     3361 ttcgaggtca tggacaacgg gtccgaccga cggcggtggt cggcatcgcc tgcagcgtcg
     3421 catcccgttg gtgagtcagc ctgaacagga gacggtcgtt gggcgagtct cgccatgcct
     3481 gacccgaacg cgcgcggcta gggtggcgtc atgctggtct ccggctccgc aaccgtgggc
     3541 gccgcacccg acgaggtgtg gaaggcgctc aacgacccgg ccgtgctcgt gcgcaccatt
     3601 cccggatgcc agcgcctcga ggagatcggc ccggattcct acacgatgac cgtgactgca
     3661 ggcgtcgcct cgatcaaagg tgtgtacacc ggccaggtgc ggctcaccga ccagcagccg
     3721 ccggacacgt tcgtgctcaa ggccagcggt gccggaggtc ctggcaccgt cagtgccgac
     3781 gtgcgggtca cgttgcgcg
//
LOCUS       DATBFZ010000333         3000 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_4709701, whole genome shotgun sequence.
ACCESSION   DATBFZ010000333 DATBFZ010000000
VERSION     DATBFZ010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 3000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3000
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4709701"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..804
                     /locus_tag="VH419_10275"
     CDS             <1..804
                     /locus_tag="VH419_10275"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEY7044042.1"
                     /translation="YVTGRQLKPVTPLWLDIRQCARDPVWDVPGTGGKGSTSSRSVDI
                     PAPYSGRLVAGTGHLHGGGIDLRLSNTSCHQTLFVSRPTWAHQEPRPLLHEGSPSHMT
                     TFSSGVGIPVRAGDELRLTATYDDSLPHTRVMGIMLVYLVPGKTPRCATIPTLQLIAD
                     KPGPPPLVPMPLPRAPKGPVDHVRSATVQDFRITPERITIPRGTLFTWHFQGVIAHNI
                     TVVNGPVGFSSPSLTAGNTFSHRFTRPGIYKIVCSLHPVAMVQEITVHR"
     gene            841..1407
                     /locus_tag="VH419_10280"
     CDS             841..1407
                     /locus_tag="VH419_10280"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009081551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0106141 - flavin prenyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HEY7044043.1"
                     /translation="MRLVVGMSGSSGAIYGIRLLEVLKDEPAVEVHLVVSGPARQTIS
                     LETDYRPADVEKLADVAYKPGDLAAAISSGSYPVDAMVVAPCSVRTASAIAWSLDDNL
                     VVRAAGVTLKERRPLVLAVRESPLHLGHLRALTQLSEMGAVVAPPLPAFYIRPQTIGE
                     IVDHTVGRLLDLVGITPPADLIRRWQGP"
     gene            complement(1364..2245)
                     /locus_tag="VH419_10285"
     CDS             complement(1364..2245)
                     /locus_tag="VH419_10285"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEY7044044.1"
                     /translation="MLRAPQFSICQFTTLHASFADDLEAYAAAGASGIGVLELKLTPG
                     DEERLRDSVLRPTVCVPSVPSILPLPLLPGPSDPAVRIESLCASIHRLARLEPVAVMF
                     LTGPGESGRRIVVEGIKRLAEASAEAGVMLAIEPFHASQRDTFSFVNSIPDALELLAE
                     ADVDGLRLLFDTWHLGDAPQIEAEIAEHASLVACVHVSDRRRPTRAEFDRVLPGEGVL
                     PLPQLFRALGESGYDGYYEVEIFSDDGTFGEALPDSLWALDPAVVAGRARSTFDRVWR
                     DAWPTVPASGESDPRAE"
     gene            2286..2699
                     /locus_tag="VH419_10290"
     CDS             2286..2699
                     /locus_tag="VH419_10290"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEY7044045.1"
                     /translation="MAAELPELVRQRLQEPTYWHLACIDTDGRPQASPMWVDLRDDRI
                     LINTAKSHRKYASLKENLDVGLSNNDATDNPFDHVQIRGRVVSVVEGQEAEDDIDDLA
                     RKYIPGAERYDWRAPGEERVTFLIEPTHVRHYRPG"
     gene            complement(2684..>3000)
                     /locus_tag="VH419_10295"
     CDS             complement(2684..>3000)
                     /locus_tag="VH419_10295"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HEY7044046.1"
                     /translation="EDTLVDKPTYDALFATSLEEDLRRRGIDTLVVAGVTSHCCVDAT
                     VQAAFVRGFDVLVLSDCVAAFADETDLHEATLHVLALLFAVVAPSAAFLEELARPGYP
                     GR"
BASE COUNT          559 a         1053 c          943 g          445 t
ORIGIN      
        1 tacgtcacgg gcagacagtt gaagcccgtg acgcctctgt ggctcgacat ccggcagtgt
       61 gcgagagatc cggtctggga tgtccccggc accggcggca agggctcgac cagctcacga
      121 agcgtcgata ttccggcccc gtactcggga agactcgttg ccgggacggg gcacctccac
      181 ggtggcggca tcgacctccg cctctccaac accagctgcc accaaacgct gttcgtctcg
      241 cgtccgacct gggcgcatca ggagcccaga ccgctgctgc acgaaggcag cccctcgcac
      301 atgaccacct tctccagcgg tgtcggcatc ccggtcagag ccggcgacga gctgcgcctg
      361 accgcgacct acgacgacag cctcccgcac acccgtgtga tgggaatcat gctcgtctac
      421 ctcgtgcccg gcaagacacc ccgctgcgcg accatcccga cgctccagct gatcgccgac
      481 aaaccgggtc cgcccccgct cgtgccgatg ccgctgccac gtgcaccaaa gggccccgtc
      541 gaccacgtac gaagcgccac cgtccaggac ttccggatca cacccgaacg aatcaccatc
      601 ccacgcggca ccctcttcac ctggcacttc cagggcgtca tcgcccacaa catcaccgtc
      661 gtcaacggcc cggtcggatt ctcctcaccg tcactcacag cggggaacac cttctcccac
      721 cgcttcacac gccccggcat ctacaagatc gtctgctcgc tccaccccgt cgccatggtc
      781 caagagatca ccgtccaccg gtagccgccc agcccgaaag aggctggtta aggtctgccg
      841 atgcgactgg tcgtcgggat gtcgggttcc agcggagcga tctacggcat ccgcctgctg
      901 gaggtcctga aagacgagcc tgcggtcgag gtgcacctcg tcgtcagcgg acctgctcgg
      961 cagacgatct cgctcgagac cgactatcgg ccggcggacg tggagaagct cgccgacgtg
     1021 gcgtacaagc cgggcgacct ggcggcggcg atctccagcg gctcgtatcc ggtcgacgca
     1081 atggtcgtgg cgccgtgctc ggtgcgaacc gcctcagcca ttgcctggtc gctcgacgac
     1141 aacctcgtcg tccgtgcagc aggggtgaca ctcaaagaac ggcgacctct ggttctggcc
     1201 gttcgagaat ctccgctcca cctcggtcac ctgcgcgcgt tgacgcaact ctcggagatg
     1261 ggagcagtcg tcgcgcctcc gctgcccgcg ttctatatcc gcccgcagac gatcggcgag
     1321 atcgtcgatc acacagtcgg ccgcctgctc gatctcgtcg gcatcactcc gcccgcggat
     1381 ctgattcgcc gctggcaggg accgtaggcc aggcgtcgcg ccagacccgg tcgaaggtcg
     1441 agcgcgcacg tcccgctacc accgccggat ccaacgccca cagcgagtcc ggaagagcct
     1501 cgccgaaggt cccgtcgtcg gagaagatct cgacctcgta gtacccgtca tagccgcttt
     1561 ccccgagagc gcgaaagagc tgcggcagcg ggagaacccc ttcgcccggg agcacgcgat
     1621 cgaactcggc gcgggtcggc cggcggcgat ccgaaacatg cacacaggcg acgagcgacg
     1681 catgctccgc gatctcggcc tcgatctgcg gggcatctcc gaggtgccac gtgtcgaaga
     1741 gcagacgcaa cccgtcgacg tcggcctccg ccagcagctc gagcgcgtcc gggatgctgt
     1801 tgacgaagga gaacgtgtcg cgctgacttg cgtggaacgg ctcgatcgca agcatgactc
     1861 cagcctcggc ggaagcctcg gcgagcctct tgattccttc gacgacgatg cggcgcccgc
     1921 tctcccccgg ccccgtgagg aacatgacgg ccaccggctc gagccgtgcg aggcggtgga
     1981 tgctcgcaca gagcgactcg attctcacgg ccgggtcgct cgggccaggc agcaggggaa
     2041 gcgggaggat cgaggggacg gagggaacgc aaacggtcgg ccggagaacg ctgtcgcgta
     2101 gtcgttcctc gtcgcccgga gtcagcttga gctcgagtac gccgatcccg ctcgcgccgg
     2161 ccgccgcgta cgcctccagg tcgtcagcga agctcgcatg aagggtcgtg aactgacaga
     2221 tcgagaactg cggcgcgcgc agcatcgatc ggagaaacta acggaattcg aagaaagggg
     2281 gacatgtggc agcggaactg ccggagctcg tccgtcaacg actgcaagag ccgacctact
     2341 ggcacctggc ctgcatcgac acggacggca ggcctcaggc atcaccgatg tgggtcgacc
     2401 tgcgcgacga ccggattctc atcaacaccg ccaagagcca ccgcaagtac gccagcctga
     2461 aggagaacct cgatgtcggg ctgtccaaca acgacgccac cgacaacccg ttcgaccacg
     2521 tccagattcg tgggcgcgtc gtgtcggtag tcgaaggaca ggaggccgaa gacgacatcg
     2581 atgacctggc gcgcaagtac atccccggag cggagcgcta cgactggcgg gcgcccggag
     2641 aggagcgggt gacgttcctc atcgaaccca cccacgtccg ccactaccgg cccggatagc
     2701 cgggccgagc cagctcttcg agaaacgccg ccgatggcgc cacgacggcg aagagcagcg
     2761 cgaggacgtg cagcgttgcc tcgtgcaggt cggtctcgtc tgcgaacgcc gccacacagt
     2821 cggacaggac gaggacgtcg aagccccgga cgaaggcagc ctggaccgtg gcatcgacgc
     2881 agcaatgtga cgtgacgcct gcgacgacga gcgtgtcgat cccgcgccga cgcaggtcct
     2941 cctcgaggct ggtggcgaac agggcgtcgt atgtcggctt gtcgacgagt gtgtcctccc
//
LOCUS       DATBFZ010000334         9707 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_3721913, whole genome shotgun sequence.
ACCESSION   DATBFZ010000334 DATBFZ010000000
VERSION     DATBFZ010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 9707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9707
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3721913"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1488)
                     /locus_tag="VH419_10300"
     CDS             complement(<1..1488)
                     /locus_tag="VH419_10300"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HEY7044047.1"
                     /translation="MGAPRLTVQVLGPLAARVDGETLNLGGRRQRAVLAVLLLARGHL
                     VTVDAIVDAVWGDDLPANAPAALQAYVSHLRRALEPDRRARDRASIIVSEGSAYALRL
                     DNDAVDAWNFERKLDAAGYARAAEQARLLTEALALWRGPALLEYADQDFARSEAERWT
                     ELRTVAREQLLAARLDLGEAPLVIPEIEALLRDEPLREERWRLLVLALYRAHRQADAL
                     AALRRARSTLADELGVDPGPALRALEAEVLAQSDRLDPPSPPDPPQPSTSMTDTTKSG
                     ATKTATKPAQIGARIPAAGPETVIVDRDRELAVLRSRLTDALGGGGGVAVIEGPAGIG
                     KSRLLAEVAREAKDAGATVLRARGSQLEREYGLGVVRQLFEPVIGDEIARERWLSGAA
                     AGASGVFDAGSHEHRSDGFFGVLHGLYWLAVNVSAAAPLVVAVDDLQWCDTGSLRFLG
                     YLTRRLEGLPILLVTTLRTGEPHDVEDLLTEITDDPLTISVAPVPL"
     gene            1643..2182
                     /locus_tag="VH419_10305"
     CDS             1643..2182
                     /locus_tag="VH419_10305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044048.1"
                     /translation="MSRHGRADSLRGRPDCWSATGWLLAVTAICGVADFLALPDGAVW
                     FGMPLSGDEMFRPEWLVMATVLAYPLVRLAQASYILGVLGFLLSAGEMLTIVDNGRER
                     YLHNAELFGGGPGFPTVWYAVAVLQGVVFLLATSHGLRRRWAARRWERMMRKLTKGTV
                     STGLAGRAGDVSRPEQVAD"
     gene            complement(2368..3312)
                     /locus_tag="VH419_10310"
     CDS             complement(2368..3312)
                     /locus_tag="VH419_10310"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEY7044049.1"
                     /translation="MSAPRHKGQRRPSTTARWLRRLSVPAAVLGVVAAGGLGFLVAPD
                     HDRSVASQTPSGHRSLGQATVEPRDERARISRSVQRPISHQPPGESGLATMAVPDVTG
                     HDWAAADLNLWSGPGEDSDLVGEIPAGTRLATTGIVEDGWAQVIVDGMPRWVNASYVS
                     QDDPDATSAADPAQVSTKQASIPDRTGSVSFAPCPSGDAVESGLTEDAVRVHRAVCAA
                     FPQVTSYGGVRPGDSGEHGTGQALDIMVSDQGLGDQIASYLIAHRSQLGVSEVLWWQQ
                     IWTVERSSEGWRAFSDRGSPTANHYDHVHVTVYGDAGR"
     gene            3708..3998
                     /locus_tag="VH419_10315"
     CDS             3708..3998
                     /locus_tag="VH419_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007079228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEY7044050.1"
                     /translation="MSASPGYADDKAAHLRRLRRVEGQVRGLQRMVEQDEYCIDVLTQ
                     VSAATKALEAFALSLLDEHLRHCVTDAVQAGGSEADDKLTEASAAIARLVRS"
     gene            4140..4679
                     /locus_tag="VH419_10320"
     CDS             4140..4679
                     /locus_tag="VH419_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008357037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HEY7044051.1"
                     /translation="MSGTLSGKTIAFVTANEGVEQVELTEPWKAVEEAGGTPKLVSIE
                     SGKVQAFNHLDKADTFSVDATFDDVAVDDFDGLLLPGGVANPDRLRMEDKAVAFIRGF
                     FDAGKPVAAICHAPWTLIEADVVKGRTLTSWPSVRTDLTNAGASWVDEEVVHDSGLVT
                     SRKPDDLPAFCATVVKFFA"
     gene            4754..5167
                     /locus_tag="VH419_10325"
     CDS             4754..5167
                     /locus_tag="VH419_10325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044052.1"
                     /translation="MGRIKTVSTKAVKYGPQIALVWKYAAVPVTAAAQRAFAAQLNRR
                     TALRHAETVNEGAILMVMDQGEVHWVVFSGGKPVAAYPSSDKPLDDLIADANLSKKMT
                     PDQFRIRQAEASRRQKALDTARSLSTQLRRRDDGM"
     gene            5249..7111
                     /locus_tag="VH419_10330"
     CDS             5249..7111
                     /locus_tag="VH419_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020660386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HEY7044053.1"
                     /translation="MQSALWIVVLAATVTLVSGLSRRAGVPAPLFLTLVGLVASYVPG
                     VPDVEMTPDLVLVGFLPPLLYAAALKTSLVDFRANRRPIALLSVGLVVGTTLVVGVVT
                     HWMLPVDWPSAFALGAVVAPPDAVAATAIARRVGMPRRMVTILEGESLVNDATALVAL
                     RTALVAGAGAVSMWEVGLDFVWAAAGGVAVGVLVAIVTGKVRKRIEDEITQTAVSFVT
                     PFLAYLIAEPLHASGVLAVVVTGLILGHKSHLMQSASSRIFERTNWTTIEFVLQNTVF
                     LLIGLQVSSILDAAADSSLGAERIVLACVVVTLSVMLVRPLWVFPSTYLPRLIPSIRA
                     VDPPPPWTYPAAVSWAGMRGVVTLAAVFVLPSDTPQRDVLVLIALVVVAATLLLQGST
                     LPWVLRRLGLSGPDPAEDALQAASVSQRAASAGLRRLDEIRTDEDSDAVIERLERRSS
                     ERVEALWERLGSAAETPSQAYARLRIAMIEAERQELIRLRDEGVVPDEVMRRVIASID
                     VEETVLDLGESWSSVERDEDLRPPEAVGGCEHLARCDKTPAPKTPQGCEECLRDGLEW
                     VHLRLCLECGHVGCCDSSIGKHADVHHGETGHPVMRSFEPGEAWRWCYVDEVLG"
     gene            complement(7118..7594)
                     /locus_tag="VH419_10335"
     CDS             complement(7118..7594)
                     /locus_tag="VH419_10335"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017934897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEY7044054.1"
                     /translation="MAKLRETVSRLLGRAGRTRRTPSGDRVNISYRPRADGRPDPGEV
                     VWTWVPFEDDPRRGKDRPVLVIGRHGTATDRLVGLMLTSKDHDRDAAQEATWGRYWMD
                     VGTGGWDRQGRPSEVRLDRLLDIDARRVRREGAALDRATFERVIAAARSHHRLTEV"
     gene            7829..>9707
                     /gene="hrpA"
                     /locus_tag="VH419_10340"
     CDS             7829..>9707
                     /gene="hrpA"
                     /locus_tag="VH419_10340"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009081503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HEY7044055.1"
                     /translation="MTVQPTTVEPLPFTIRYPDQLPVSQRKDDIAAAIRDHQVVIVAG
                     ETGSGKTTQLPKICLDLGRGRNGRVIGHTQPRRIAARTVAERIAEELGVELGAYVGYK
                     VRFTDRSSDRTSVKVMTDGILLAEIQRDRDLRRYDTIIVDEAHERSLNIDFILGYVKQ
                     LLPRRPDLKLIITSATIDPERFSRHFGGAPIIEVSGRTFPVEVRYRELAEDQTQGITD
                     AVGELMREGPGDILVFCSGEREIRDAADALKKLRLPRPTDTLEVLPLFARLSSAEQHR
                     VFQPHSTRRVVLATNVAETSLTVPGIHYVVDTGLARISRYSARTKVQRLPIEPISQAS
                     ANQRKGRCGRVADGICIRLYAEDDFAARPEFTDPEILRTNLASVILQMTALGLGDVAR
                     FPFVEPPDTRSVAAGVQLLEEIGAISRKRNAHQLTRMGRQLARLPIDPRLGRMMVEGS
                     RRGCLREVITVVAALSMQDPRERPSDQQELADQKHRRFADPDSDFAGVLRLWRYVKEQ
                     QDELSSSAFRRMCRAEFLNYLRIREWQDLDGQLRQVAKQLDLSLNKTPAAAEAIHQSL
                     LAGLLSHIGMRDEEKRDYVGARGTRFAIFPGSSLFKKQPAFVMSAELVETARLWGRMN
                     AR"
BASE COUNT         1481 a         3268 c         3406 g         1552 t
ORIGIN      
        1 cagtggcacc ggtgccaccg agatcgtcag ggggtcgtcg gtgatctcgg tgagcaggtc
       61 ctcgacgtcg tgcggctcgc cggtccgcag cgtcgtcacg agcaggatcg ggagcccctc
      121 gaggcgtcgg gtcaggtagc cgaggaaccg cagtgagccg gtgtcgcacc attgcaggtc
      181 gtcgacggcg acgaccagcg gcgccgccgc cgacacgttc acagcgagcc agtacagccc
      241 gtgcagaact ccgaagaagc cgtcgctgcg gtgctcgtgg gacccggcgt cgaacacgcc
      301 actcgcgccg gcagccgctc ccgagagcca gcgttccctg gcgatctcgt cgccgatgac
      361 cggttcgaac agctgccgca cgacgccgag cccgtactca cgctcgagct ggctgccgcg
      421 cgcgcgaagc acggtcgcgc ccgcgtcctt cgcctcacga gcgacctccg cgagcaaccg
      481 gctcttgccg atgcccgccg gcccctcgat cacggcgacc ccgccgccgc cgccgagggc
      541 gtcggtcagc ctggatcgga gtacggcgag ctcgcggtcg cggtcgacga tcaccgtttc
      601 cggaccggcg gcaggaatgc gggcaccgat ctgggctggc ttcgtcgccg tcttcgttgc
      661 gccgctcttc gttgtgtcgg tcatcgaggt gctgggttgc ggtgggtcgg gcgggctggg
      721 tgggtccagc cggtcgctct gggcgagcac ctcggcctcg agtgcgcgca gggccggtcc
      781 cgggtcgacg ccgagctcgt cggcgagggt gctacgagcc cttcgcagcg cggcgagcgc
      841 atccgcctgc cggtgggctc ggtacagggc gaggacgagc agtcgccacc gctcctcgcg
      901 cagcggctcg tcgcgcaaca aggcctcgat ctctgggatc accagcgggg cctcgccgag
      961 gtcgagcctg gccgccagga gttgctcgcg ggcgacggtc ctcagctcgg tccaacgttc
     1021 cgcctcggac cgggcgaagt cctgatcggc gtactcgagc aacgccggtc ctcgccacag
     1081 cgccagggcc tcggtcagca gtcgggcctg ttctgccgcc cgcgcgtagc ccgctgcgtc
     1141 gagcttccgt tcgaagttcc acgcgtcgac cgcgtcgttg tcgaggcgaa gggcgtatgc
     1201 ggagccctcg ctgacgatga tgctcgcacg gtcgcgcgcc cgccggtccg gctcgagcgc
     1261 tcggcgcagg tgggacacgt aggcctgcag cgcggcgggc gcgttcgccg ggaggtcgtc
     1321 gccccagacg gcgtccacga tcgcgtccac ggtcacgaga tgcccgcgcg cgagcagcag
     1381 cacggccaac acggcccgtt ggcggcgtcc gccgaggttg agcgtctcgc cgtccacccg
     1441 tgcggcaagg ggcccgagca cctggacggt caggcgaggc gcacccacgg ccgaaactct
     1501 agggtgcggt accgcgcagc gcgggcggat tgccggaatc gggccgcagg gtcgcgctgt
     1561 caggtcgcga tccgataacg acgggattcc gattcacatc gcgatgggtg cagtgtcggt
     1621 ggcgcgcagc acgatgttgg gcatgtcccg ccacgggagg gccgactcgc ttcgaggtcg
     1681 gcccgactgc tggtctgcca cgggatggct gctggccgtc accgcgatct gcggtgtggc
     1741 ggacttcctt gcgctgccag atggtgctgt ctggttcggt atgccgttgt ccggagacga
     1801 gatgttccgg cccgagtggt tggtgatggc gaccgtgctg gcgtacccgc tggttcggct
     1861 cgcccaggcc agttacatcc tcggagtgct cggtttcctg ttgtcggccg gcgagatgct
     1921 cacgatcgtc gacaacgggc gggaacggta cctgcacaac gccgaactct tcggtggcgg
     1981 tccagggttc ccgacggtgt ggtacgccgt cgcggtgctc caaggcgtcg tcttcctcct
     2041 cgcgaccagc catgggctgc gacgccgttg ggccgcccga cgctgggagc ggatgatgcg
     2101 gaagttgacc aagggcaccg tctcgacggg ccttgccggg cgcgctgggg acgtctcccg
     2161 gcccgagcag gtagccgact agggtgccgg ctccgttcgc cgactaggtc ctccgagccg
     2221 cggcctccgc aagggtgggt cggaggccgt ggctcgtgat gacacaccgc cctcccgccg
     2281 aggtggcagg ttttgttgct gttggtcggc gtgtcgaacc gcgattgcca cctcgatctc
     2341 ctccccgatc aggtgaccgg ctggacgtca gcgtcccgcg tcgccgtaca ccgtgacatg
     2401 cacgtggtcg tagtggttgg ccgtcggcga gccccggtcg gagaatgctc gccagccctc
     2461 actgctgcgt tcgaccgtcc agatctgctg ccaccacaag acctcgctga cgccgagctg
     2521 tgacctgtgg gcgatcaggt aggaggcgat ctggtcgccc agcccctggt cggacaccat
     2581 gatgtcgagc gcctgacccg taccgtgttc gccggagtca ccaggccgga ccccgccgta
     2641 cgacgtgacc tgcgggaacg ccgcgcacac ggcacggtga acacgaaccg cgtcctccgt
     2701 cagcccgctc tcgacggcat cgcccgacgg gcagggcgcg aacgaaacgc tgccggtgcg
     2761 gtcggggatg gacgcttgct tggtcgaaac ctgcgcgggg tcggctgcgc tggtcgcgtc
     2821 ggggtcgtcc tggctcacat acgaggcatt gacccagcgc ggcatgccgt cgacaatcac
     2881 ctgcgcccag ccgtcctcca cgatcccggt ggtcgccagc ctcgttccgg cgggaatctc
     2941 accgaccaga tcggagtcct cgccagggcc cgaccacagg ttgaggtcgg cggcagccca
     3001 gtcatgtccc gtgacgtccg ggacggccat ggtcgcaagg ccggactcgc cgggtggctg
     3061 atgcgagatc ggtcgctgca cggaccggga gatccgtgca cgctcgtcgc gcggctcgac
     3121 ggtcgcctga ccgaggctgc ggtgtcctga tggtgtctgg ctcgcaacgg atcggtcgtg
     3181 gtccggcgcg accaggaagc cgagcccgcc ggctgccacc acgcccagga cagctgcggg
     3241 cacggacagc cggcgcagcc accgggccgt cgtggatgga cgtcgctgac ccttgtgacg
     3301 gggtgcggac acggacgaat caccgatcca gacgttgacg aggcgctggc gagacgccgc
     3361 cactggggcg ggtcaaagtc cgagtgaacc acagctgaga cgtctcggcc caactcgaca
     3421 tctcgcgtgg tggtttgcct gggtagccat ccgaacgcac gggtagccag gcagatctgc
     3481 cttgcgaccg gtgggtttcc ctggtgcggg tggcaaccag cgcgcagggg tttgcgatgt
     3541 ccacatccca aactccctgt gtccatcgaa ggtttgcgat gtttacacgc cgtttgggct
     3601 gtaaacatcg caaaccaccc ccaagcaggc ggccccgcac cgaaggtgta cggtgagcgg
     3661 tggccgcaga tacccccctt cggtatccaa gggaatggga ggaccggatg tccgcatcac
     3721 ctgggtacgc cgacgacaag gctgcgcatc tacgccgact acgccgggtc gaagggcagg
     3781 tccgtgggct gcagcgcatg gtcgagcagg acgagtactg catcgacgtg ctgacccagg
     3841 tctccgccgc cacgaaggcg ctcgaggcct tcgcgttgtc gctgctcgac gagcacctgc
     3901 ggcactgtgt gaccgatgcc gtgcaggcgg ggggcagcga ggcggacgac aagctcaccg
     3961 aggcgtcggc cgccatcgcg cgtctcgttc gttcctgaca ttcaagcacc ggctgggtcg
     4021 gtggacactc gtcccgagct gatcgtgacg gtgagacatg cgccacccgg cgtttcaagc
     4081 actcgcagtg ggagacagaa cgggaaacag cccacccaat ccctcgaagg gagcgcccca
     4141 tgagcggaac gctcagcggc aagacgatcg cgttcgtgac agccaacgaa ggtgtcgagc
     4201 aggtcgagct caccgagccg tggaaggccg tcgaggaagc cggcggcacg cccaaacttg
     4261 tatcgatcga gagcgggaag gtccaggcgt tcaaccacct cgacaaggcg gacacgttct
     4321 cggttgacgc caccttcgac gacgttgccg tcgacgactt cgacggcttg ctgcttcccg
     4381 gcggcgtcgc caacccggac cggctgcgca tggaggacaa ggcggtcgcg ttcatccgcg
     4441 gattcttcga cgccggcaag ccggtcgctg cgatctgcca cgcaccgtgg acgctgatcg
     4501 aggctgacgt cgtcaagggc cggaccctga ccagctggcc ctcggtccgc accgacctga
     4561 ccaacgccgg cgccagctgg gtggacgagg aggtcgtcca cgacagtgga ctcgtgacca
     4621 gccgcaagcc ggacgacctg ccggcgttct gcgcgaccgt ggtgaagttc ttcgcctagc
     4681 gagacctacg atgagtcttg cgccggggcc ctcgtgaccg cggcccgagg gtgcgtcgag
     4741 gaaggctgtg gcggtgggtc ggatcaagac cgtcagcacg aaagcggtga agtacgggcc
     4801 gcagatcgcc ctcgtctgga agtacgccgc cgtgccggtc acggccgccg cgcaacgcgc
     4861 gttcgccgcc cagctcaacc ggcgtacggc gctcagacac gccgagacgg tcaacgaggg
     4921 cgcgatcttg atggtcatgg atcaaggcga ggtccattgg gtcgtgttca gcgggggcaa
     4981 gccggtggcc gcctatccct cgtcggacaa accgctcgac gacctcatcg cggacgcgaa
     5041 cctgtctaag aagatgacgc ctgaccagtt ccgaatccgc caagctgaag cgtcgcggcg
     5101 gcagaaggcg ctggacacgg ccagatcctt gtcgacgcag ctgcgccgtc gcgacgacgg
     5161 catgtgacct tccggccatg actgtccggc gactgtccgg cggctgagcg acgcaggcgg
     5221 gcgccgcgcc gtctgggaaa ctcgacgtgt gcagtccgcc ctgtggatcg tcgtcctcgc
     5281 cgcgacggtc accttggtct cgggcttgtc gcggcgggcg ggggttccgg cgccactgtt
     5341 cctcacgctc gtcgggttgg tggcgtcgta cgtgccgggt gtccccgatg tcgagatgac
     5401 tccggatctc gtgctggtcg gtttcctgcc gcccctgctg tacgccgccg cgctcaagac
     5461 gtcgctcgtc gacttccggg cgaaccggcg gccgatcgcg ttgctgtcgg tgggactggt
     5521 cgtcggcacg acgctggtcg tcggtgtcgt cacgcactgg atgctccccg tggactggcc
     5581 ctcggcgttc gcgctcggag ccgtcgtcgc ccctccggat gccgtcgctg ccacggcgat
     5641 cgcgcgccgt gtcggtatgc cgcggcggat ggtcacgatc ctggaggggg agagccttgt
     5701 caacgacgcg acggcgctgg tcgcgctgcg caccgcactc gtcgccggcg cgggagcggt
     5761 gtcgatgtgg gaggtcggcc tcgacttcgt ctgggcggca gccggtggag tggcagtcgg
     5821 cgtactcgtg gccatcgtca cggggaaggt ccgcaagcgg atcgaggacg agatcaccca
     5881 gaccgcggtg tcgttcgtga caccgttcct cgcgtacctc atcgccgagc cgctccacgc
     5941 gtcgggagtg ctggccgttg tcgtgacggg cctcatcctc ggtcacaagt cgcatctgat
     6001 gcagtcggcg tcctcgcgga tcttcgagcg gaccaactgg acgacgatcg agttcgtgct
     6061 gcagaacacg gtgttcttgc tcatcgggct acaggtgagc tcgatcctgg acgcggcggc
     6121 cgatagctcg ctgggagccg aacgcatcgt gctcgcctgc gttgtcgtca cgttgtccgt
     6181 catgttggtg cggccgctct gggtcttccc gtcgacgtac ctaccgcgac tcatcccgag
     6241 catccgagcg gtcgatccgc cgccgccgtg gacctaccct gcggcggtct cgtgggcggg
     6301 catgcgtggt gtcgtgacgt tggcggcggt cttcgtcctt ccctcggaca cccctcagcg
     6361 cgatgtgctt gtcctcatcg cgctggtggt ggtcgcggcg acgttgctcc tgcaggggtc
     6421 gacgctgcca tgggtcctgc gacggctcgg actcagtggg cccgacccgg cggaggacgc
     6481 gctgcaggca gcgagcgtgt cgcagcgggc ggcatcggcc ggcctgcgtc ggctggacga
     6541 gatccggacc gacgaggact ccgatgcggt catcgagcgg ctggagcgta ggagcagcga
     6601 acgtgtcgag gcgttgtggg agcggctcgg cagcgcggct gagaccccca gccaggcgta
     6661 cgcgcggctc cggatcgcga tgatcgaagc cgaacggcag gagctgatcc ggctgcgcga
     6721 cgaaggggtg gtccccgacg aggtcatgcg tcgggtgatc gcgtcgatcg acgtcgagga
     6781 gaccgtgctc gatctcggcg agtcgtggag cagcgtcgag cgtgacgagg acctgcggcc
     6841 gccagaagcc gtcggcgggt gcgagcacct cgcgcggtgc gacaagacac cggcgccgaa
     6901 gaccccgcaa ggatgcgagg agtgtctgcg ggacgggctc gagtgggtgc acctgcggct
     6961 gtgtctcgag tgcggccacg tgggctgctg tgactcctcg atcggcaagc acgctgacgt
     7021 gcatcacggc gagaccggtc atccggtgat gcgcagtttc gaaccgggtg aagcatggcg
     7081 ctggtgctac gtcgacgagg tcctcggata gtcccgctca gacctcggtc agtcggtggt
     7141 ggctgcgggc ggcggcgatc acacgctcga acgtcgcgcg atccaaggcg gcgccctcgc
     7201 gacgtacgcg gcgggcgtcg atgtcgagca gacggtccag gcgtacctcg ctcggccggc
     7261 cctgccggtc ccaacctcca gtgccgacgt ccatccagta gcggccccag gtcgcttcct
     7321 gggcggcatc gcggtcgtgg tccttgctgg tgagcatgag cccgaccagc cggtccgtgg
     7381 ctgtgccgtg ccggccgatg accaggacgg gtcggtcctt gccacgacgc gggtcgtcct
     7441 cgaacggcac ccacgtccag accacctcgc ctgggtcggg acgaccgtcc gcccggggcc
     7501 gataggagat gttgacgcgg tcgccggagg gtgtacgacg agtgcggccg gcgcgaccga
     7561 gcagccgact gaccgtctcg cgcagctttg ccatgaagtg accctaggtc accgtgtccg
     7621 gtgccgacct gaggcgttcc gcaggggcgc tggttgcgat aggcggcgcc gcggtttgcc
     7681 acggggacga ggcaagcccg cacgtcccaa ggcagatctg cctggctacc catgcgtttg
     7741 gacgggtagc caggcaaacc accacggaca catgcatcag tagcagccgc ggcggccgcg
     7801 gccgcctgat ccgtatcctg ggagcctgat gaccgtgcag cccacgaccg tggagccgct
     7861 gccgttcacc atccgctacc ccgaccagct gccggtctcg cagcgcaagg acgacatcgc
     7921 cgccgcgatc cgcgaccacc aggtcgtcat cgtcgcgggg gagacgggca gcggcaagac
     7981 gacgcagctt ccgaagatct gcctcgacct cgggcgcggt cggaacggac gcgtgatcgg
     8041 tcacacccag ccgcgccgga tcgccgctcg cacggtggcg gagcggatcg ccgaggagct
     8101 cggcgtcgag ctcggcgcgt acgtcggcta caaggtgcgc ttcaccgatc gatcgagcga
     8161 ccgtacgtcg gtcaaggtga tgaccgacgg gatcctgctg gccgagatcc agcgcgaccg
     8221 agacctgcgc cgctacgaca cgatcatcgt ggatgaggcg cacgagcgca gtctcaacat
     8281 cgacttcatc ctcgggtacg tcaagcagct gcttccacgc cggccagacc tgaaactgat
     8341 catcacctcc gcaacgatcg acccggagcg attctcgaga cacttcggcg gcgctccgat
     8401 catcgaggtg tcgggtcgca cgttcccggt cgaggtgcgg taccgcgagc tcgccgaaga
     8461 ccagacccag ggcatcaccg acgcagtcgg agagctgatg cgggaggggc cgggcgacat
     8521 cctcgtgttc tgctcgggtg agcgtgagat ccgcgacgcc gccgacgcgt tgaagaagct
     8581 gcgcctccct cgtccgaccg acacactcga ggtgctgccg ctgttcgcgc gattgtcgtc
     8641 ggccgagcag caccgggtct tccagccgca cagcacgagg cgggtggtgc tcgccaccaa
     8701 cgtggccgag acgtcgctca cggtccccgg aatccactac gtcgtggaca ccggcctggc
     8761 ccgcatctcc cgctacagcg cgcgcaccaa ggtccaacgg ctgccgatcg agccgatctc
     8821 gcaggccagc gccaaccagc gcaagggccg ctgcggtcgg gtcgccgacg gcatctgcat
     8881 ccggctgtac gccgaggacg acttcgcggc cagaccggag ttcaccgacc cggagatcct
     8941 gcggaccaac ctcgcctcgg tcatcctcca gatgaccgca ctcggcctcg gggacgtcgc
     9001 ccgcttcccg ttcgtcgagc cgccggacac ccgaagcgtg gccgcgggtg tgcagctgct
     9061 cgaggagatc ggggccatca gcaggaaacg caacgcccac cagctgacca ggatgggccg
     9121 gcagctcgcg cggctgccca tcgacccgag actgggccgg atgatggtgg aggggtcacg
     9181 acgtggctgc cttcgcgagg tcatcacggt ggtcgcggca ctgtcgatgc aggacccacg
     9241 cgagcgcccg tccgaccaac aggagctggc cgaccagaag catcgccgct tcgcggaccc
     9301 ggactcggac ttcgccggag tgctcaggct ctggcggtac gtcaaggagc agcaggacga
     9361 gctgtcctcg agcgcgttcc gccggatgtg cagggcggag ttcctcaact acctccgtat
     9421 ccgcgagtgg caggacctgg acggccagct gcggcaggtc gccaaacagc tcgacctctc
     9481 gctgaacaag acgcccgccg ccgccgaagc catccatcag tcgttgctcg ccggtctgct
     9541 gtcgcacatc ggcatgcggg acgaggagaa gcgtgactac gtcggcgccc ggggcaccag
     9601 attcgcgatc tttcccggct cgtcgttgtt caagaagcag ccggcgttcg tgatgtccgc
     9661 cgagctcgtc gagaccgctc gcctctgggg acggatgaac gcgcgga
//
LOCUS       DATBFZ010000335         7208 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_558632, whole genome shotgun sequence.
ACCESSION   DATBFZ010000335 DATBFZ010000000
VERSION     DATBFZ010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 7208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7208
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_558632"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="VH419_10345"
     CDS             <1..456
                     /locus_tag="VH419_10345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044056.1"
                     /translation="RTFLLAACGILAVGLPSHVMGATMHLTMHGLATSTVPTHLQVTV
                     VDQLQGLGAVYFGLILPFLLGLVLLTAALWRARVVGWQPFALLLGDIVFGMTVNGSHT
                     PSDWMWWIDPILTITAFAWMGVGVVRYRETTGRVAVPRDVERPLERAAG"
     gene            515..1810
                     /locus_tag="VH419_10350"
     CDS             515..1810
                     /locus_tag="VH419_10350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044057.1"
                     /translation="MATQQPRRAPSRAGQVGFAAAVGGLAGAALTAHRGARPAALGSI
                     VGAGALATSEAIARTQQRLGEIPPLWQRIAVSGALAAPFGWLLGRLGHVGPIGVGVVT
                     GSIVGVLGVRPQKVVLGPAVGLVVGRAGAMSRLAAPTALVASATVLTYRSLAALLFRD
                     AQVTLLAERVRAEDLPFVVPLPARSRYVGTGYVRELAAATGGAYTEDASDTGIVASLD
                     ALAGPEFDPATVDPRVREFYEHTTRFSLDIDPEWRAWVRPGYLLYRTVVARPLGQASV
                     PMNQREALRGVRSRIDTIAPTPDEAVSVRGWIRSYADNDEPIYVGVYTTYRHQGSGYV
                     SVGFPVPQGSFTATLLPRGRAGGGLTLTSRTHDGQAGHYLTYIDPESDELTALAVHGF
                     AEHLDVYVDNGQLRAEHAFWVFGLPFLVLHYRIEPKPIA"
     gene            complement(1824..3161)
                     /locus_tag="VH419_10355"
     CDS             complement(1824..3161)
                     /locus_tag="VH419_10355"
                     /inference="COORDINATES: protein motif:HMM:NF021524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
                     /protein_id="HEY7044058.1"
                     /translation="MRGSPEAASVRREATWEYVKGALWVLPTLAVVLALAVGSLVSQI
                     DVDPTSTWGKFVFRGTAQEARDLLIGIAGTMVTVIALVLGLTVVALQLASTQFSPRLL
                     RSFLRDRTNQVVLSVFVATFTYSSAGLYTVGVSASGPVADYPRAAVTGALVLLFASMV
                     MLVFFAHHLAHSIQIDEVMRQVERSTLHVIEHDFPTVDTAVPLPEVPEHARTIPAYAS
                     GYVQTIHPEILVGPAEGLDAVLSVRPMIGEHVVEGGPLVHIWAGHGVTPPSPPPALVE
                     LVRGAIRIGFERTAEQDVALGIRQLADVASKALSPAINDPYTAVQALDRLSVILSRLA
                     GCTLGNQVLAGADGTVRVAVPARDLEYYLDLGCGQVRRFGASEPRVMSALLRVLDNTG
                     RCAATDEDRSLVARQVELVLDDAERRTAQPADLISIQRTANALMEHLRAPAGR"
     gene            3300..5507
                     /locus_tag="VH419_10360"
     CDS             3300..5507
                     /locus_tag="VH419_10360"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEY7044059.1"
                     /translation="MSSARRRTAVSLVAASATVALAFVAVQPTMAARGNAPGAPGQPS
                     DWNAGDKDGFGTAVGTASKVWYTLNGGELTDVYFPRIDTPSIRDSQFVVTDGSTFTDR
                     EDRDSTHSVTLVSPDSLTYQLVNTATSGRWRITKTFVTDPARSSVLEDVRFESLTGKP
                     YQLYLLHDIALSMTGNDDTGVTGDGPSLLSSDGTNASAVVTSPALGKTSSGYLGTSDG
                     WTDLASDHDLDGQYDATTPGNVVQTGRVKVNGLQGHQHVTVAVGFAPSQPGDSTTAQA
                     MAALSTARASLAGGFDSARSAYDAGWAQYLSTLTPVPESAARWTTEWKVSAMVLAASE
                     DKTFRGGFVAAPGRPWAWANSLQEFAVYHAVWSRDLYQIATALLADGDEAAAGRALDY
                     LWNVQQRPDGSFPQNSRLDGAPVFGGLQLDEVAFPIVLAHQLGRTGPADWAHVKKSAD
                     YIVANGPSTPQERWENIGGYSPATIAAEIAGLVCAADIARANGDPASASTYLSTADTW
                     QRNVESWTATPNGPYRPRPYYLRITANGDPDVGTQIQISDGGPLIDQRYVVDPSFLEL
                     VRLGVKPAHDRTVINSLAVVDQKLAYLTANGPFWHRASFDGYGEKRDGSQWESVPAGS
                     GITVGRGWPLLSGERGEYSLVAGSRLAARVLLGTMGRAADNHSHLMSEQVWDRNPPAP
                     SPGFVPGEPTFSATPLAWTHAQFLRLASSIDAGSPVETPQVVACRYGSEACQR"
     gene            5590..>7208
                     /locus_tag="VH419_10365"
     CDS             5590..>7208
                     /locus_tag="VH419_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020387797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEY7044060.1"
                     /translation="MTEAIDEYVVRRLAEWDRDLTLAATWRDPTAEASDVERLVACFD
                     AQLQSRHLDLAARWLQQQGEGFHTVGSAGHESDAAVALALRPTDPALLHYRSGGFYAA
                     RAQQVDGTTPVTDVLLGMAAAADDPISGGRHKIFGNAALSVIPQTSTIGSHLPRAVGL
                     AFSLDRATRLGLSTPWPADAVVVASFGDASVNHSTAVGALNAAAYCVHRGVPVPLLLV
                     CEDNGFGISTPTPEGWVDQALSRWPGIDCAEADGVRPAELLRCADGLVQQVRRERRPA
                     ILHLRTVRFMGHAGSDAELGYRSRTEIERDYRRDPLLSAAATLVCLGAFTPQDALQRY
                     EEARQTVMTEASKLVGVRRLASADEVMGPLTARHPTAALAAARRTRAVPPEEQAPPVT
                     LAGAINATLADLLAVWPQAVVFGEDVAIKGGVYGVTRGLRKRFGGLRVFDTLLDEQTI
                     LGTALGAALAGLLPIPEIQYLAYLHNAADQIRGEGASLAFFSKGQYRNGMVVRVPGLA
                     YQKGFGGHFHNDNSLAVLRDIPGVVVAVPSHP"
BASE COUNT         1107 a         2462 c         2491 g         1148 t
ORIGIN      
        1 cggaccttcc tcctcgcggc atgcgggatt ctggccgtgg gcctgcccag ccacgtcatg
       61 ggtgccacca tgcacctcac gatgcacggg ctcgccacgt cgacggttcc gacgcatctg
      121 caggtcaccg tcgtcgatca gctgcagggg ttgggtgcgg tctacttcgg gctcatcctg
      181 cccttcctgc tcggcctcgt gctgctcacg gcggcgctgt ggcgcgcacg cgttgtcggc
      241 tggcagccgt tcgcactgct gctcggcgac atcgtgttcg gaatgacggt caacggcagc
      301 cacacgccgt cggactggat gtggtggatc gacccgatcc tgaccatcac ggcgttcgcg
      361 tggatgggcg tcggcgtcgt gcggtatcgc gagaccactg gtcgcgtcgc ggtgcctcgc
      421 gatgtcgagc gaccgctgga gcgcgcggcc ggctgactgc cacattgcgc gggcacacgg
      481 tcggatcgag tcgctcgggc gataggttcc gagggtggcg acacaacaac ccaggcgtgc
      541 gcccagccga gcgggtcagg tcgggttcgc cgccgcggtg ggcgggctcg ccggtgcggc
      601 gctcaccgca caccgcggtg cccggcccgc cgctctcggc tcgatcgtcg gcgcgggcgc
      661 gctggcgacg tccgaggcga tcgctcgtac gcagcagcgg ctcggcgaga tccccccgct
      721 ctggcagcgc atcgctgtca gcggtgcgtt ggcggcgcct ttcggctggc tgcttggtcg
      781 cctcggtcac gtgggtccca tcggcgttgg cgtggtgacc ggttcgatcg tcggcgtgct
      841 cggcgtacgg ccgcagaagg ttgtgttggg gcccgcggtg ggtttggttg tcggacgcgc
      901 aggcgccatg tcccgccttg cggcaccgac cgcactggtc gcctccgcca cggtgctcac
      961 ctatcgatcg ctggccgcgc tgctgttccg ggacgcgcag gtgacgctgc ttgctgaacg
     1021 ggtgcgtgcc gaggacctgc cgttcgtggt tccgctgccc gctcgctcgc ggtacgtcgg
     1081 caccggctac gtgcgtgagc tggcggccgc gaccggtggc gcgtacaccg aggacgcctc
     1141 ggacaccggg atcgtcgcgt cactcgacgc gttggcgggg ccggagttcg atcctgcaac
     1201 cgtcgaccca cgtgttcgcg agttctacga gcacaccacg aggttctccc tggacatcga
     1261 ccccgagtgg cgggcctggg tgcgtccggg atacctgctg taccgcacgg tggtcgcgcg
     1321 cccactcggg caggccagtg tcccgatgaa ccaacgggag gcgttgcgcg gtgttcgcag
     1381 caggatcgac acgatcgcac cgactccgga cgaggcggtg tcggtgcgcg gctggatccg
     1441 ctcgtacgcc gacaatgacg agccgatcta cgtcggcgtc tacacgacct accgccatca
     1501 gggcagtgga tacgtcagcg tcgggtttcc ggttccacaa gggagcttca ccgcgaccct
     1561 cctgccgcgt ggccgtgccg gcggtgggtt gacgctgacc agccgcacgc acgacggtca
     1621 ggccgggcac tacctcacct acatcgatcc ggagtccgac gagctcactg cgttggccgt
     1681 ccacgggttc gccgagcacc tcgacgtgta cgtcgacaac gggcagctgc gggccgagca
     1741 tgcgttctgg gtgttcggtc tgccgttcct cgtcctgcac taccgcatcg agcccaagcc
     1801 gatcgcttga gtggccgggc tcttcatcgt cctgccggtg cacggaggtg ctccatcaat
     1861 gcgttggcgg tgcgctggat cgagatcagg tcggccggct gcgccgtccg ccgttccgca
     1921 tcgtccagga cgagctccac ctgacgcgcg acgagtgacc tgtcctcgtc cgtggccgcg
     1981 cagcgacccg tgttgtcgag gacgcggagc agcgccgaca tgacccgggg ttctgacgca
     2041 ccgaaccgcc tcacctgacc acacccgagg tcgaggtagt actcgaggtc gcgggcggga
     2101 acagccaccc ggacggttcc gtcggcgccg gccagcacct ggttccccag tgtgcagccg
     2161 gccagccggg acaggatcac cgagagacgg tcgagcgcct ggaccgccgt gtacgggtcg
     2221 ttgatggccg gcgagagcgc cttcgatgca acatcggcga gctgtcggat gcccagcgca
     2281 acgtcctgct cggcggtgcg ctcgaagccg atccggatgg cgccgcggac gagctcgacc
     2341 agtgccggcg gcggcgacgg aggcgtcacg ccgtgcccgg cccagatgtg gacgagcggg
     2401 ccgccctcga cgacgtgttc accgatcatc ggccgcaccg acagcacggc gtcgagaccc
     2461 tcggccgggc cgacgaggat ctccgggtgg atcgtctgga catagccgga ggcgtacgcc
     2521 ggaatcgtcc gggcgtgctc aggaacctca ggaagcggga ccgcggtgtc gacagtcgga
     2581 aagtcgtgct cgatcacgtg cagcgtcgaa cgctcgacct ggcgcatcac ctcgtcgatc
     2641 tggatcgagt gcgccagatg atgggcgaag aagaccagca tgaccatgct cgcgaacagc
     2701 aggaccaagg ctcccgtgac agccgctcgt ggatagtctg cgaccggccc gctcgctgag
     2761 acgccgacgg tgtagagacc ggccgagctg taggtgaacg tcgcgacgaa gacgctcagc
     2821 accacctggt tcgtgcggtc acgaaggaag ctacggagca gccgcggcga gaactgcgtc
     2881 gacgcgagct ggagtgccac cacggtgaga ccgagcacga gcgcgatgac ggtcaccatc
     2941 gtgccggcga tcccgatgag caggtcacgc gcctcctgag ctgtcccgcg gaagacgaac
     3001 ttgccccagg tcgacgtcgg atcgacgtcg atctgcgaga cgagggatcc gactgcgagg
     3061 gccagcacca cagcgagcgt ggggagcacc cacagcgctc ccttcacgta ctcccacgtc
     3121 gcctcgcggc ggacgctggc ggcctcaggt gaaccacgca tcacattcga gcatctcgcc
     3181 cttcccgggc gaccggttca tacggaacgg gtgaccgcgc tcaacgagcg agccgcacgc
     3241 agccgtccta cggtggaggg atcgtacgca ccctgcgagc ttgtctggag tgacctctcg
     3301 tgtcgtcagc acgtcgtcgt actgccgttt cccttgtcgc cgcctcggcc accgttgctc
     3361 ttgccttcgt cgctgtgcag cccaccatgg cggctcgggg caacgccccc ggagcccccg
     3421 ggcaaccgag tgactggaac gcaggtgaca aggacggctt cggcactgct gtcggtacgg
     3481 cgagcaaggt ctggtacacg ttgaacggcg gcgagctcac cgacgtctac ttcccgcgca
     3541 tcgacacacc cagcatccgt gacagccagt tcgtcgtgac ggacggttcg acgttcactg
     3601 accgggaaga ccgcgactcg acgcactcgg tcacgttggt gagcccggac agcctgacct
     3661 atcagctcgt caacaccgca acgtccggga ggtggcggat caccaagacc ttcgtgacgg
     3721 acccggcacg gtcgtcggtg ctcgaggacg tccggttcga atcactcacc gggaagccgt
     3781 accagctcta cctgctgcac gacatcgcgc tgtcgatgac gggcaacgac gacacaggag
     3841 tcaccgggga cggaccgtcg ctgttgtcct ccgacggcac caatgcgagc gcggtcgtga
     3901 cctctccggc gctcggcaag acgtccagcg gatacctcgg tacgtccgac ggctggaccg
     3961 atctggcctc cgaccacgac ttggacgggc agtatgacgc aacgaccccg ggcaacgtcg
     4021 tgcagaccgg ccgggtgaag gtgaacggtc tgcaaggcca tcaacacgtc acggtcgcgg
     4081 tcggcttcgc accaagccag ccaggagaca gcaccacggc ccaggcgatg gcagcgcttt
     4141 ccacggcccg tgcatcgctc gccggcggct tcgacagcgc gcgttcggcg tacgacgccg
     4201 gctgggcaca gtacctgtcg acgctgaccc ccgtcccgga gagcgcggcc aggtggacga
     4261 ccgagtggaa ggtctcggcc atggtgctgg ctgccagcga ggacaagacg ttccgcggtg
     4321 gcttcgtcgc cgcgccggga cggccgtggg cgtgggcgaa ctcgctgcag gagttcgcgg
     4381 tgtaccacgc cgtctggtcg cgggatctct accagatcgc gacggcgctt ctcgccgacg
     4441 gggacgaggc agccgcgggg cgagcgctgg actacctgtg gaacgtgcaa cagcggcccg
     4501 acggatcctt cccgcagaac tcgaggcttg acggtgcacc ggtgttcggc gggctgcagc
     4561 tcgacgaggt ggccttcccg atcgtgctgg ctcatcagct cggccggact ggcccagccg
     4621 actgggcgca cgtcaagaag tcggctgact acatcgtcgc gaacggcccg tcgactccgc
     4681 aggaacgatg ggagaacatc ggcggctact caccggcgac gatcgcggcg gagatcgccg
     4741 gactcgtctg cgccgctgac atcgcgaggg cgaacggcga cccagcgagc gcttcgacgt
     4801 acttgtcaac cgccgacacg tggcaacgga acgtcgagtc gtggacagcc acccccaacg
     4861 gcccctaccg accgcggccg tactacctgc gaatcacagc gaacggggat ccggacgtcg
     4921 gcacccagat ccagatctcc gacggtgggc cgctcatcga ccagcgctac gtggtggacc
     4981 cgagcttcct cgagctcgtg cgactgggtg tgaagcccgc ccacgaccgg acggtcatca
     5041 actcgctggc agtggtcgac cagaagctcg cctatctcac cgccaacggt ccgttctggc
     5101 accgggcgtc gttcgacggg tacggcgaga agcgcgatgg ctcacagtgg gagtcggtcc
     5161 cggcaggctc cgggatcacc gtcggccggg gctggccgct gctcagcggt gagcgcggcg
     5221 agtactcact cgtcgccggc tctcggctcg ccgcacgcgt cctgctcggc acgatgggac
     5281 gagccgcgga caaccacagc cacctgatgt ccgaacaggt gtgggaccgg aacccaccgg
     5341 caccttctcc aggtttcgtg ccgggagaac cgacgttctc ggcgaccccg ctggcgtgga
     5401 cgcacgcgca gttcctccgg ctggcctcct cgatcgacgc gggttcgccc gtcgagacac
     5461 cccaggtggt cgcctgtcgc tacggcagcg aggcctgcca acgttgacgc cggcttgcgt
     5521 ccgtgaaagt cgccgctagc gcgaccggtg gtacggacgt tggcgggtgg agcacgcgaa
     5581 gatggcggca tgaccgaggc gatcgacgag tacgtcgtcc gacggcttgc cgagtgggac
     5641 cgtgacctga ccctggcggc gacgtggcgc gacccgacgg ctgaggcgtc ggacgtggag
     5701 cgactggtcg cgtgcttcga cgcacagctc cagagccggc acctcgacct ggcggctcgc
     5761 tggctgcaac agcagggaga gggtttccac accgtcggct cggccggcca cgagagcgac
     5821 gcagccgtgg cgctggcact ccggccgacg gacccggccc tgctgcacta ccggtccggc
     5881 ggcttctacg ctgctcgcgc gcagcaggtc gacggcacga caccggtcac ggacgttctg
     5941 ctcggcatgg ccgcggccgc cgacgatccc atctcggggg gacggcacaa gatcttcggg
     6001 aacgcggcct tgtcggtgat ccctcagacc tcgacgatcg ggtctcatct gccgcgcgca
     6061 gtcgggctcg ccttctcctt ggaccgcgcc acccgacttg gcctgtccac gccgtggccg
     6121 gcggacgcgg tcgtcgtcgc cagcttcggc gacgcgtcgg tcaaccattc gacggcggta
     6181 ggagctctca acgccgcggc gtactgcgtc caccgtggcg tcccggtgcc gctgctgttg
     6241 gtctgcgagg acaacggctt cggcatcagc accccgacac cggaaggctg ggtcgatcag
     6301 gcgttgtccc gctggcccgg catcgattgc gccgaggctg acggtgtccg gccagcagag
     6361 ctgctgcggt gtgccgatgg tctggtgcag caggtccgcc gcgagcgccg gcccgccatc
     6421 ctgcacctgc gcacggtccg gttcatgggc cacgccggct ccgacgctga gctcgggtac
     6481 cggtcgcgga cagagatcga gcgcgactac cgccgggacc cgttgctgtc ggctgctgcc
     6541 acgttggtgt gcctcggtgc gttcacgccg caggatgcct tgcaacggta cgaggaggcg
     6601 cgtcagaccg tgatgacgga agcctcgaag ctcgtcggag tacgccgcct cgccagcgcg
     6661 gacgaggtga tgggaccgct gaccgctcgg caccccacgg ctgcactggc cgcggcccgg
     6721 cgtaccaggg cagtcccgcc tgaggagcag gcgccgccgg tgaccctggc aggtgcgatc
     6781 aacgccactc tcgccgatct gctcgcagtg tggccgcagg ctgtcgtgtt cggcgaggac
     6841 gtggcgatca agggtggggt gtacggcgtg acccgtggtc tgcgcaagcg gttcggcggg
     6901 ctgcgcgtct tcgacaccct gctcgacgag cagacgattc tcgggacagc actcggcgcg
     6961 gcgctcgcgg gtctgttgcc gataccggag atccagtacc tggcctacct ccacaacgcg
     7021 gcggaccaga tccgtggcga gggtgcctcg ctggcgtttt tctcgaaagg tcagtaccgc
     7081 aacggcatgg tggtccgggt tcccggcctc gcgtaccaga agggattcgg cgggcacttc
     7141 cacaacgaca actcgctcgc cgtgctacgg gacatccccg gtgtggtcgt ggcagtgccg
     7201 tcgcatcc
//
LOCUS       DATBFZ010000336         2842 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_4382612, whole genome shotgun sequence.
ACCESSION   DATBFZ010000336 DATBFZ010000000
VERSION     DATBFZ010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2842
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4382612"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(185..256)
                     /locus_tag="VH419_10370"
     tRNA            complement(185..256)
                     /locus_tag="VH419_10370"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(222..224),aa:Val,seq:cac)
     gene            532..951
                     /locus_tag="VH419_10375"
     CDS             532..951
                     /locus_tag="VH419_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016467747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsgA family sporulation/cell division regulator"
                     /protein_id="HEY7044061.1"
                     /translation="MDIAPVSVSHPLTLELIDAHGRSTPLEAELCYDSFDPYAVAACF
                     DTGEACVRWVFARELLACGLYEPTGDGDVHVWPCLDASGRAVTIIELSSPDGEALMQA
                     RSDDVTNFLRRTESLVPSGTEGELVDVDDTIARLLAS"
     gene            1082..1654
                     /locus_tag="VH419_10380"
     CDS             1082..1654
                     /locus_tag="VH419_10380"
                     /inference="COORDINATES: protein motif:HMM:NF018984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HEY7044062.1"
                     /translation="MTFSHDTEHALLVVVDLVNTAPECGGVEALPDSGALAAWVRRHH
                     ISGAEPSDFSDVTSIHQVRDRFRACFGVDDVEVLTTRVNALVAEAPVRPRLTDHDGYD
                     WHIHYFAPGVTLAEHLAIDGGMALAHVVTAAETDRLRVCEAPDCEAVLLDLSRNRSKR
                     YCDARTCGNRLNVAAYRERKRTAALATGPS"
     gene            complement(1777..2592)
                     /locus_tag="VH419_10385"
     CDS             complement(1777..2592)
                     /locus_tag="VH419_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012920967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HEY7044063.1"
                     /translation="MRAWCNGSLVEPTDPAVSLLDHGFTVGDGVFEAIKVVDNQPFAL
                     TRHLRRLTRSATGLGLPEPDHDQVRRGIEATIEGQHLAYGRIRVTYTAGISPLGSNRN
                     ITDPTVAVVTAKATPFEPSTKGVVVPWPRNERAATAGLKTTSYADNVVALAYAAERSA
                     SEAIFANTAGNLCEGTGSNVFCVFDGTVVTPPLSAGCLAGITRELLIEWYDVHERDIS
                     MAEFAEADEVFFTSTTRDIQAIHDIDGRPLDIHKVTERVIEEWRAREVQDLDP"
     gene            2726..2798
                     /locus_tag="VH419_10390"
     tRNA            2726..2798
                     /locus_tag="VH419_10390"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2759..2761,aa:Gly,seq:gcc)
BASE COUNT          429 a          957 c          956 g          500 t
ORIGIN      
        1 gcgagtctag ggaggcgtga gcggtggggt aagccgattg tggagaccgc tcgggcgttt
       61 cggaggttcc ccgcgagcaa ggcggggaac aagcaccggg aacaagcgtg agcgagttcc
      121 ccgtatgttc cccggaaaca catcaggccc ggcataaacc gggcctgacc tgcggaaact
      181 cgggtgggcg atactgggtt cgaaccagtg acctcttcgg tgtgaacgaa gcgcgctacc
      241 actgcgccaa tcgccctggc ccggcgtacg ccgctgacgt ctgtggacgt actgggaacg
      301 acggaaaagg ctagcgcacc agggcgcgtc gggcgaattg cctctcccct atcgggtgta
      361 ccgacgactc cgccatcgtc cgtccggcca tagttcctcg acttgctccg cgaacgtccg
      421 ggtttgccgg tcagatgatg ccgtacgtga aggattcttt gcggtatcag cgtcacgaga
      481 tgggcactac tgcgttatcc atgtgaccgg ctgaaggaag gcacagggat tatggacatc
      541 gcacccgtct cggtgagtca cccgctcacc ctcgaactga tcgacgcgca tgggcgctcg
      601 actcccctcg aggccgagct gtgctacgac agtttcgacc cctacgcagt cgccgcctgc
      661 ttcgacaccg gcgaggcctg cgtccggtgg gtcttcgcgc gtgagctgct cgcctgcggc
      721 ctgtacgagc cgaccggcga cggcgacgtc cacgtctggc cctgcctcga tgcgtccggc
      781 cgagccgtca cgatcatcga gctctcgtca cctgacggcg aggcgctcat gcaggcgcgc
      841 agtgacgacg tgaccaactt cctgcgccgt accgagtcgc tcgtcccgag cggcaccgaa
      901 ggtgagctcg tcgacgtcga cgacacgatc gcccgattgc tcgcttcctg accgatctgt
      961 ccggcttccg tcgccggtgc cgccgacacg cccctcgccc gctcgatcct cgctgccagc
     1021 cttcctcagc gccgtccgcg atcgcgccgg tctcctggtc cggggtgtct accctggtgg
     1081 cgtgacgttc agccatgaca ccgagcacgc gttgctcgtg gtcgtcgacc tggtcaacac
     1141 cgccccggag tgcggcggtg tcgaagcgct gcccgactcc ggtgcgctgg cggcgtgggt
     1201 acgccggcac cacatctccg gcgccgaacc ttccgacttc tcagacgtga cctcgatcca
     1261 tcaggttcgc gatcggttcc gagcctgctt cggggtcgat gacgtcgagg tgctgacgac
     1321 gcgcgtgaac gcgctggtgg cggaggcccc ggtccggccc aggctcaccg accacgacgg
     1381 gtacgactgg cacatccact acttcgcgcc cggcgtcacg ctggcggaac acctggccat
     1441 cgacggcggc atggccctgg cccacgtggt gaccgcagcg gagaccgacc ggctgcgcgt
     1501 gtgtgaggca ccggactgcg aggcggtgct gctggacctg tcccggaaca ggtcgaagcg
     1561 ctactgcgac gcccggacct gcggcaaccg tctcaacgtc gcggcgtacc gcgaacgcaa
     1621 gcgcaccgcc gccctcgcca ccggcccgtc ctgacgctct cccgtccggt tccggttccc
     1681 cgactgccgg gcttccgcga attgcgggaa aggtcttgcg cgcagcggcg tatcgacgtg
     1741 cactttcccg catttcgcgg ggacctgagt ggagcgtcag gggtcgaggt cttggacctc
     1801 ccgggcgcgc cattcctcga tgacgcgctc ggtgaccttg tggatgtcca gcggacggcc
     1861 gtcgatgtcg tggatggcct ggatgtcgcg agtggtcgag gtgaagaaga cctcgtccgc
     1921 ctcggcgaac tctgccatcg agatgtcccg ctcgtggacg tcataccact cgatgaggag
     1981 ttccctcgtg atcccggcga gacagcctgc cgacagtggt ggcgtgacga cggtgccgtc
     2041 gaacacgcag aagacgttcg agccggtgcc ctcgcacagg ttgccggccg tgttggcgaa
     2101 gatcgcctcc gacgcgctgc gctcggcggc gtacgcgagg gcgacgacgt tgtcggcgta
     2161 cgacgtggtc ttcagccctg ccgtggcggc ccgctcgttg cgtggccacg ggacgaccac
     2221 ccccttcgtc gagggctcga acggggtcgc cttcgcggtc accacggcca cggtcgggtc
     2281 tgtgatgttg cggttcgacc ccagcggcga gatcccggcg gtgtaggtga cgcggatccg
     2341 cccgtaggcg aggtgctgac cctcgatcgt ggcctcgatt ccccgtcgta cctggtcgtg
     2401 gtcgggctcg ggtagtccca gccctgtggc cgaccgggtg agccggcgca ggtgccgggt
     2461 gagcgcgaac ggctggttgt ccacaacctt gatcgcctcg aacacaccgt cgccgacggt
     2521 gaagccgtgg tccagcaggg agaccgccgg atcggtcggt tcgacaagcg acccgttgca
     2581 ccatgcacgc atggcgctac cgtagccgga taggtcgtgg cgagggcgct ggccggaatt
     2641 tgtcccacgg accaaatcgg ctaatgttct ctctcgcaac gccccgtacg gccggccagt
     2701 tggccgccgt cgtacggcgt cacctgcgga cgtagctcag ttggtagagc gcaaccttgc
     2761 caaggttgag gtcgcgagtt cgagcctcgt cgtccgctcg gagcgggtct cgagcccatc
     2821 cagcggtggg ttggccgaga gg
//
LOCUS       DATBFZ010000337        19639 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_4710854, whole genome shotgun sequence.
ACCESSION   DATBFZ010000337 DATBFZ010000000
VERSION     DATBFZ010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 19639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19639
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4710854"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1202)
                     /locus_tag="VH419_10395"
     CDS             complement(<1..1202)
                     /locus_tag="VH419_10395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044064.1"
                     /translation="MKSMPIAVRRSLVAASAAMLVSTTIAGVAPAQTHRDHPAARAAA
                     PAASTPMLKLIHAADRVTLDRYGQRVYLALGVYAAAGDLPFEIRAHRPSWAKPIQAFL
                     ETPGGEVALPAHSFSDFQRLRRFFTVSFTNPVGDVVWDRTFGFCPAGEAVRVDPEAPT
                     PSRPYPAECPYNPFTRGAVYGISPGHALPLLGDYGPTARIPLGHYKATVTIADRYADV
                     LGLTPAQAKQVIRVHVVKARGDCRTEEAARGCKADLRPETVGRGDGVPRTPGERPTGP
                     ALANAPAQIRPDLGSLPAYAVSLHRGYVSFAATVWNAGPSPLVVDGFRRTNEDVMDAY
                     QYFFDADGNEIGHAPAGEMEWDARHGHQHWHFRDFARYRLLDADKQAVVRSRKEAFCL
                     ANTDAVDYT"
     gene            complement(1393..2199)
                     /locus_tag="VH419_10400"
     CDS             complement(1393..2199)
                     /locus_tag="VH419_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008359177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HEY7044065.1"
                     /translation="MTERKPVESWLTDMDGVLVHEERAIPGAAEFIEKLRTSGRRFLV
                     LTNNSIFTPRDLRARLLASGLDVPEESIWTSALATAQFLADQRQQGSAYVIGEAGLTT
                     ALHEVGYVMTTRDPDYVVLGETRTYSFEAITTAIRLIEAGARFIATNPDVTGPSPEGP
                     LPATGSVAALITRATGVDPYFVGKPNPLMMRSALNQIGAHSETTVMVGDRMDTDVISG
                     LEAGMRTILVLTGSTREHQVDRFPYRATRVVDSVADLIDLVGELDPALAG"
     gene            complement(2311..3360)
                     /gene="alc"
                     /locus_tag="VH419_10405"
     CDS             complement(2311..3360)
                     /gene="alc"
                     /locus_tag="VH419_10405"
                     /EC_number="3.5.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR02961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004037 - allantoicase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoicase"
                     /protein_id="HEY7044066.1"
                     /translation="MSETSNATRPAGFTAMPDLASRALGGSVVAANDELFAERENLIK
                     PEPAEFSPHEFGHKGKVYDGWETRRRRDDGHDWAIVRLGVPGIVHGVVVDTSWFTGNY
                     PPFVSVEAVNVDGYPTPDELLDLDWTSLLERSPAYGDTANQYAVKDRGVWSHVRLSIH
                     PDGGVARFRVHGIPVPDPRFLTGTVDLAALENGGRLVDCSDAFYSSPQNLILPGRASI
                     MGHGWENARRRGGGNDFAVFALAGEGVIDHVELDTSYFVGNAPGWMRVQACHAEPDAS
                     VADLTEATWWDVVAKQRTLPDTRHRFLVEPRPDDSRAATHVRLDVYPDGGLARMRIYG
                     RLTESGLADLRHAAG"
     gene            complement(3366..4715)
                     /gene="allB"
                     /locus_tag="VH419_10410"
     CDS             complement(3366..4715)
                     /gene="allB"
                     /locus_tag="VH419_10410"
                     /EC_number="3.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR03178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0050897 - cobalt ion binding [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HEY7044067.1"
                     /translation="MSRTVFRGRCVLPDAEREAAVVVDHGRITAIHDLEAWSPDPHAT
                     VVDLRADEVLLPGLVDTHVHVNEPGRTEWEGFETATRAAAAGGVTTIVDMPLNSIPPT
                     TDLPALEVKRAAAEGKCYVDVGFWGGAVPTNLGGLGPLYDAGVFGFKCFLLDSGVAEF
                     APLGRAELDAAMAEVAAFDGLLIAHAEDAATIEAATVDVGCSYAAFVASRPPQAEDRA
                     IAALIAAAIEHEARVHVVHLSNAGSLDRLARAKADGVRISVETCPHYLALEAELIEDG
                     ATAYKCCPPIRSHDNRERLWQGLADGVVDMVVSDHSPATPELKQPDIGDFGAAWGGIA
                     SLQLGLSVVWAQAAQRGHALHDVATWMATAPAAIVGVAGKGGIVVGHDADFAVFAPDE
                     TFVVDAARLHHRHPLTPYAGRTLRGVVRQTWLRGRPIAADGQVDPVPTGDLLCRGAQ"
     gene            complement(4712..6049)
                     /locus_tag="VH419_10415"
     CDS             complement(4712..6049)
                     /locus_tag="VH419_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008356537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEY7044068.1"
                     /translation="MSNPDSSGATTPDESARRRTDREPRLGEDDLAALDAIVAPADER
                     MARLYPGDGGGRQPVHTVYLPADRMTADVAARWGKAARRALDEHASSSKEFAAALGLS
                     QPAALEVNDGWGRILAKLATEPIEDLRIDLEDGYGIRDDDIEDRDAVAAARALTQAGI
                     LEQPLPFCGIRFKALEASTRRRGLRTLDLFLGTLLEGGDLPAGFVATLPKVTSVEQVE
                     AMVAACARLESAYGLADHRLRFEIQIETAQSILAYDGTALVARLVHTADGRCAGLHFG
                     TYDYTAALGVSATYQAMDHPAAEHAKQVMALAAAQTAVRVSDGSTNLLPVGDTDQVHR
                     AWSEHARLVRRSLQRGFYQGWDLHPAQLPSRYAATYLFFLEDLPDVLARLHRYLGLPG
                     GDGPGQHLDEPATAQALATFLVRGLDCGAIDAASVGDESVVDDVRRLAARRVG"
     gene            complement(6046..7650)
                     /gene="aceB"
                     /locus_tag="VH419_10420"
     CDS             complement(6046..7650)
                     /gene="aceB"
                     /locus_tag="VH419_10420"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020577996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HEY7044069.1"
                     /translation="MTDLAGVEVKAPQDEPRQEEILTPQALAFVADLQRMFGGRRDEL
                     LRARAERRTTISQTGTLDFLDETRDVREAEWQVAPAPVALQDRRVEITGPTDAKMAIN
                     ALNSGAKIWLADFEDANTPHWNNVVEGQVSLYDAHRHVLVFTSPDGKEYALNDGDLAV
                     PVVRPRGWHLPEKHVLVDGQPMVGGLLDFGLHFFHNAEELLQRGLGPYYYLPKMESHL
                     EARLWNDVFTHAQDVLGIPYGSIRATVLIETITAAFEMEEILFELQEHASGLNAGRWD
                     YLFSVIKNFRDAGESFVLPDRAAVTMTQPFMQAYSELLVATCHRRGAFAMGGMAAFIP
                     SRRDAEVNEKALQKVREDKQREADGGYDGSWVAHPDLVPVCMEVFDEILGDRPNQLDR
                     QRDVSVSAADLLDVAATEGDITDAGVRGNVSVAIRYLVSWLLGTGAAAIDNLMEDAAT
                     AEISRSQLWQWTHAGSKTADGTIVSAEHVRATVEEVYAELSTSWADQPATMEALDQAR
                     TLFERVALDDDFADFLTLPAYEEYVA"
     gene            complement(7762..10173)
                     /gene="pucD"
                     /locus_tag="VH419_10425"
     CDS             complement(7762..10173)
                     /gene="pucD"
                     /locus_tag="VH419_10425"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase subunit D"
                     /protein_id="HEY7044070.1"
                     /translation="MHFPAIRGYPRSGERSTAEPTTTPQTPTGAPDSLRDNTIGGVGE
                     SARRPDATLKVTGEFAYGSDLWMDDMLWGVTLRSPHPYARITGIEIGPALATAGVFAV
                     LTHEDVPGHKTYGLEVADQPVLAIDVVRYQGEPIALVAADHPDIARQAAKKIVVHYDV
                     LEPVTDARTALGHPGVVLHGDLAGEVTELSRESADLHPQGNLVRHLKVRVGDPDVTAD
                     VIVAGDYEVGMQDQAFLGPESGLAVPDGVGGIDLYVATQWLHVDQKQICSALGLRPDQ
                     VRLSLAGVGGAFGGREDLSMHVHGCMLAMHTGKPVKMVYNREESFFGHVHRHPASMHY
                     EHGATRDGKLVYAKAQIYLDGGAYASSTPAVVGNAGTMGLGPYVVPNIQVDCFGAYTN
                     NPPCGAMRGFGSVQTAYAVEAQMDKLAAALGMDPVDFRLRNAFEEGSKAPTGQVIDSA
                     APVAELLQRLTAMPLPPDLDPSDLRHLPGGVSNTTHGEGVRRGVGYAVAYKNVAFSEG
                     FDDYSTARVRLEIIAGSPHVTVHTASAEVGQGLVTVEQQICRTELGVDTVVVAPADTQ
                     VGSGGSTSASRQTYVTGGAVQAACRKVREQVFERARDYLRRPLHELRLEAGMVMSDGG
                     VVASIADVLGTDVVEETLEWRHRRTEPIDPETGQGFAHVQYAFAAHRAVVDVDVELGL
                     VKVVELACTQDVGKAINPDAVLGQIQGGSTQGMGLAVMEEIQIVDGKIRNPSFTDYLI
                     PTILDTPPMPIEVLEYADPNAPYGVRGVGEAPTISSGPAVLAAIRQACGRDLTRIPVR
                     PEHII"
     gene            complement(10251..11633)
                     /locus_tag="VH419_10430"
     CDS             complement(10251..11633)
                     /locus_tag="VH419_10430"
                     /EC_number="3.5.4.32"
                     /inference="COORDINATES: protein motif:HMM:NF006055.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine deaminase"
                     /protein_id="HEY7044071.1"
                     /translation="MNAGRSNALVLDGCAVVTMDSARTEYSVGHVVVEGARISSVGAG
                     RAPRDVGDAEYVDATGCLATPGLVNTHHHLYQWVTRGMAVDHSLFEWLTTLYPVWACI
                     DEATVNVAALGGLGWLARTGCTTTMDHHYVFPAGGGDLLAAEIDAARQVGLRFLPTRG
                     SMDLGKSQGGLPPDHVVEDIDTVLQATADAIDTHHDPSPDSMLRVGVAPCSPFSVTGD
                     LLKESAGLARDKGVRLHTHLAETVDEDDFCAQKFGCSPVEYVESLGWLGSDVWLAHGI
                     HFDDEAVQRLADTRTGVAHCPSSNARLGAGICRTRDLLDVGVPVGLGVDGAASNEAAS
                     LVEELRHAVLFARARGGPTALSVRQALELATIGGAEVLGWDDQIGSIETGKLADLALW
                     RLDTIAHADIDDPVAALVLGSAPPLELLLVQGSPVVRHDELVTVDEAAVARDVQAAST
                     ALLSRAGVTS"
     gene            complement(11630..12121)
                     /locus_tag="VH419_10435"
     CDS             complement(11630..12121)
                     /locus_tag="VH419_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008360867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEY7044072.1"
                     /translation="MRITATVNGEIHQADDVWEGESLLYVLRERMGLPGSKNACEQGE
                     CGSCTIYLDGLPVCACLVAAGQAQDREIVTVEGLAEVDDLHPIQQAFIEAGAVQCGFC
                     TPGLIVQTHDLLQRDPEPDDAEIREALAGNLCRCTGYEKILDAVRMAAGRMATAVDRG
                     GPP"
     gene            complement(12118..12999)
                     /locus_tag="VH419_10440"
     CDS             complement(12118..12999)
                     /locus_tag="VH419_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009081536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEY7044073.1"
                     /translation="MEFLAPTTLADALAAKEEIPDAMPIIGGTDVMVELNFDRHRPPA
                     LLDLTRIPELADWELDDGFVRLGAAVTYTEVIEELGAILPGLAMASRTVGSPQIRNRG
                     TVGGNLGAASPAGDSHPPLLCADTEVELASASGVRRVPIDEFYTGVKQHAGRPDELIT
                     AIVVRPPSGPQQFSKIGTRNAMVISVSAFSIALHPDRQQIRTGVGSAAPTPRRARDAE
                     DFLAGALAEGSLWESRGEIPESVAQEFGERVKAAASPIDDVRGTAAYRLHALAVMARR
                     TLAWAWADYADPGRSGR"
     gene            complement(12987..13943)
                     /gene="pucL"
                     /locus_tag="VH419_10445"
     CDS             complement(12987..13943)
                     /gene="pucL"
                     /locus_tag="VH419_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009082386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004846 - urate oxidase activity [Evidence
                     IEA];
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urate oxidase"
                     /protein_id="HEY7044074.1"
                     /translation="MTIHPEDNPSQSPATFVLQDQYGKAENRLVKVDRDSSRHVIHDL
                     NVTTTLRGDFAAAYTEGDQSKVLPTDTQKNTVFAFAKSHGVDQIEDFGLALARHFVDD
                     VAPVSGARVAIEEFGWDRIEVDGVGHDHSFVRTGEGVRTTAVTVEGRGDDQRGWVVSG
                     VQDLVVLKSTGSEFAGFLTDEFTTLAETHDRVMATSLVVRWRYDTLAAGSVVPGNWQA
                     AYDDIRATLLERYATVHSLALQQTLYEMGTAVLERQPQVVEVRLSAPNLHHFAYDLER
                     FGLTNDGEVFHADDRPYGLIQAAVSRADAPDAGPAWEARWSS"
     gene            complement(13943..14281)
                     /gene="uraH"
                     /locus_tag="VH419_10450"
     CDS             complement(13943..14281)
                     /gene="uraH"
                     /locus_tag="VH419_10450"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020387446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HEY7044075.1"
                     /translation="MSHVTTHVLDTAHGRPAVGLTVVLEHDNASATEPPAVATGETDE
                     DGRVADLGPERLDPGTYRLRFNTGAYFATAGAAAFYPEVSICFEVTDDSHYHVPLLLS
                     PFGYSTYRGS"
     gene            complement(14278..14781)
                     /gene="uraD"
                     /locus_tag="VH419_10455"
     CDS             complement(14278..14781)
                     /gene="uraD"
                     /locus_tag="VH419_10455"
                     /EC_number="4.1.1.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008358834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline
                     decarboxylase"
                     /protein_id="HEY7044076.1"
                     /translation="MQLREFNGLAADEAQRTVDPCLGVRRWVDEVVAGRPYADLAALV
                     VQAKASAENLSDGELAEALARHPRIGERADPDVAGGQEADHSRREQSGVSDHPELADR
                     LQKGNRRYEDRFGRVFLVRAAGRSGTQILDELERRLDNDDETERRETVCALREIAVLR
                     LQQVVQP"
     gene            14857..15771
                     /locus_tag="VH419_10460"
     CDS             14857..15771
                     /locus_tag="VH419_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007079244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HEY7044077.1"
                     /translation="MDHPYAERVRIVSLLPSTSEILFALGAGDSVVGVTFECDYPPLA
                     RTRRIVSTSSLPEGLEPAAIDAVVAERLAAGEDLYHLDEGALADLDADIVVTQDLCAV
                     CAVDVSVVDDALAHLGCRAEVVTIDPHTLDEVLASIDVLGKVSGTEQRAADLVESLRT
                     RLAAVESSVAGLPQPRMLVLEWTEPPFAPGHWVPEMIERAGGLSTLGAAGEKSTRTTW
                     DAVAASQPEVIVSAPCGYALTPSVDLAEKLVGTGVLPDGVPLWAVDANASFARPGPRL
                     IDGVEVLAAIAHAEACGPPDPTLAHQIR"
     gene            complement(15793..17244)
                     /locus_tag="VH419_10465"
     CDS             complement(15793..17244)
                     /locus_tag="VH419_10465"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEY7044078.1"
                     /translation="MNRRLLGIAALTTATLLPTLGAVGVTGTPSRHATTHDAVAVATE
                     NPNGIGDPYYPADGNPGYNVRRYHVRLDYFPQPESIRATTWVHAIATTRLPSFHLDLK
                     GLHVDSVAVDDRAATWRRTGQHELVVTPARPVHKLAGFVTEVAYHGKLGYVIDGGAPS
                     GWIPVGKEGSGVLEGEPHGCAVWYPCNDHPIDKARWSLAATLPRPLDLVSVGEQGPTT
                     SGTRHGVAVRTFRWRLGERTATYMTGMYIGKLTFERSTLRDGTPVVSAYGPNPGEAPE
                     RESQLPEILRVLARRWGPYPAPQAGGMFVSGFIPYELETFTRPIYSRSTGLLTIVHEN
                     GHQWWGDNVSIKRWRDICLNECLASYSEWLWDEHNGVDLDHRYRIRVARIGDSLFSGK
                     LYDMGANHEFDTPVYVKGKYFVHALRNKIGDASFFRAMRGIQRDLAGGHISMIGLRNQ
                     LERRTGVDLRSFWREWVLHTGRPSDANLFPGDL"
     gene            complement(17396..18838)
                     /locus_tag="VH419_10470"
     CDS             complement(17396..18838)
                     /locus_tag="VH419_10470"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF037667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEY7044079.1"
                     /translation="MRVRHTKQTVAVVALAALALVTTACTSDDAETASGSSPASGQGV
                     GDPYFPDDGNLGYDVIGYHVRLTYDPAHHAITARTTIIARATQRLRSFHLDLLGLHVD
                     DARVDGKPARQRRTDAHELVITPRTPIQQTRRFTTVVGYHGRPGADPVGPVQSGWYDA
                     VTPGGGFIAGEPHSCTVWYPCNDHPTDKATFALTATVPRPLEVVSVGVQGVATATTLR
                     DGTKARTYRWRLDEPTATYLTTLYIDKLTFERSTLGDGTPVVSAYGPHPRGAPQREAK
                     LPEILRVLSDAWGPYPAPAAGGIFVTAEVPFSLETYTRPLYTYGADVATIVHENAHQW
                     WGDNVSVMRWRDICLNECFASYSVWLWDEAHGADLDARYHEAVDRGGADWFDLPLYDM
                     GPAHLFDYYGVYFKGTFFLHALRNKLGDEAFFSALQGIQRDDSGGNLSMLGLRDELER
                     RTGVDLTSFWQEWVIDTGRPSDANLYPGDL"
BASE COUNT         2870 a         6735 c         6818 g         3216 t
ORIGIN      
        1 gtgtagtcga ccgcgtcggt gttggccagg cagaacgcct ccttgcggct gcgcaccacg
       61 gcctgcttgt cggcgtcgag caggcgatag cgggcgaagt cgcggaagtg ccagtgctgg
      121 tggccgtgcc tcgcatccca ctccatctca ccggccgggg cgtggccgat ctcgttcccg
      181 tcggcgtcga agaagtactg gtaggcgtcc atgacgtctt cgttggtacg ccggaagccg
      241 tcaacgacga gcggcgaagg tccggcgttc cacacggtag cggcgaacga gacgtacccc
      301 cggtgcagcg agaccgcgta cgccggaagg ctgcccaggt caggcctgat ctgggcaggt
      361 gcgttcgcga gcgctggacc ggtcgggcgc tcaccagggg tcctgggtac gccgtcgcct
      421 cgaccgactg tttctggtcg caaatctgcc ttgcaaccac gcgccgcctc ttcggtccgg
      481 cagtccccgc gcgccttcac cacgtggacg cgaatgacct gcttggcctg tgcaggcgtc
      541 agaccgagca cgtccgcgta cctgtcggcg atcgtcaccg tcgccttgta gtggcccagc
      601 gggatccggg ccgtgggacc gtagtcgccg agcagcggca acgcatgtcc ggggctgatc
      661 ccgtagaccg cgccccgcgt gaaggggttg tacgggcact ccgccggata cggccgcgac
      721 ggggtgggcg cctccgggtc gacccggaca gcttcgcccg ccggacagaa gccgaacgtg
      781 cggtcccaga cgacgtcgcc gacagggttc gtgaacgaga ccgtgaagaa ccgccgcagg
      841 cgctggaagt cagagaagga atgtgccggc agcgccacct ctccgccggg cgtctcgagg
      901 aacgcctgga tcggcttggc ccacgacggc cggtgcgcgc ggatctcgaa cggcaggtct
      961 cccgcggccg cgtagacgcc gagggcgagg tagacacgtt gcccgtaccg gtccagcgtc
     1021 acccggtccg cagcatggat cagcttgagc atcggtgtcg aagccgctgg tgcggccgct
     1081 ctggccgctg gatgatcgcg atgagtttgt gccggcgcga ctccggcgat ggtcgtcgag
     1141 acgagcatgg ctgccgaagc cgcgacgaga ctgcggcgta cggcgatggg catcgacttc
     1201 atggcgtccc ctctgatgtc cccatgggtg aggtgtccgc cgcgaagcca cgacggatcg
     1261 tggtcacatc caacacgagc gcatgccgcg ggaccgtcat gcacgtcagt tggtgtcgag
     1321 gttgtttgcc ctattcgacg cgccgaggcg cggataggtt gcctgccgcc ggtgactgga
     1381 atccgtggac ggctatccgg cgagcgctgg gtcgagctca ccgaccagat cgatgaggtc
     1441 ggccaccgag tcgacgaccc gggtggctcg gtaggggaac cggtcgacct gatgttcacg
     1501 cgtcgatccg gtcagcacga ggatcgtccg catacccgcc tcgagccccg agatgacatc
     1561 ggtgtccatt cggtcaccga ccatgacggt cgtctcgctg tgcgcgccga tctggttgag
     1621 tgcgctgcgc atcatcagcg ggttgggctt gccgacgaag tacgggtcga ccccggtggc
     1681 ccgggtgatc agcgccgcga ccgaaccggt cgccggaagc ggtccctccg gggacgggcc
     1741 ggtcacgtcg gggttggtgg cgatgaaccg ggcgcctgcc tcgatcagcc ggatcgccgt
     1801 cgtgatggct tcgaaggaat acgtgcgcgt ctcaccgagg acgacatagt ccgggtcccg
     1861 ggtcgtcatg acgtacccga cctcgtgcag cgccgtcgtc agaccggctt cgccgatgac
     1921 gtacgccgac ccctgctgcc gctggtcggc gaggaactgg gccgtggcca gcgccgacgt
     1981 ccagatcgac tcctcaggca cgtcgaggcc ggacgccagc aggcgagccc ggaggtcccg
     2041 aggcgtgaag atcgagttgt tcgtcagcac gaggaaccgg cgcccggacg tccggagctt
     2101 ctcgatgaac tccgcggctc ccgggatcgc gcgctcctcg tggacgagga caccgtccat
     2161 gtcggtgagc cagctctcga ccggcttgcg ctccgtcata cgtcaatcgt tccagggtcc
     2221 ggatacctgc acggactggg ctgaccgggg attgcgggaa ggatcacgtt ctcgtcggcg
     2281 tgtcggccgc cactttcccg caattcgcgg ttaccccgcg gcatggcgca gatcggcgag
     2341 accagactcg gtcagccgtc cgtagatacg catccgggcc aggccgccgt cgggatagac
     2401 gtccagccgg acatgcgtcg ccgctcgcga gtcgtccggc cggggctcga cgaggaaccg
     2461 gtgccgcgtg tccggcagcg tccgctgctt cgcgacgacg tcccaccacg tcgcctcggt
     2521 caggtccgcc accgaagcgt ccggctcggc gtgacaggcc tggacgcgca tccagccggg
     2581 cgcgttgccg acgaagtacg acgtgtcgag ctcgacatgg tcgatgaccc cttccccggc
     2641 gagcgcgaac accgcgaagt cgttgccgcc gccgcgccgt cgggcgttct cccagccgtg
     2701 tcccatgatc gaggcgcggc cgggaaggat gaggttctgc ggcgacgagt agaacgcgtc
     2761 cgagcagtcg acgagtcggc ctccgttctc cagcgctgcg aggtcgaccg tgccggtgag
     2821 gaacctcggg tccggtacgg gtattccgtg gacgcggaac ctcgccactc cgccgtcggg
     2881 atggatcgac agccggacat gggaccacac accccggtcc ttgacggcgt actggttggc
     2941 ggtgtcgccg tacgcgggcg aacgctccaa gagcgaggtc cagtcgaggt cgagcagctc
     3001 gtccggcgtc gggtaaccgt cgacgttcac ggcttcgacc gagacgaacg gcgggtagtt
     3061 gccggtgaac caggacgtgt ccaccacgac gccgtgcacg atccccggca cgccgagccg
     3121 cacgatggcc cagtcgtggc cgtcgtcgcg tcgacgccgc gtctcccagc cgtcgtacac
     3181 cttgcccttg tggccgaact cgtgcggcga gaactccgca ggctctggct tgatgaggtt
     3241 ctcgcgctcg gcgaagagtt cgtcgttcgc ggcgaccacg ctgccgccca gggctctcga
     3301 cgcgaggtcc ggcatggcgg tgaagcccgc cggacgggtg gcgttcgacg tctcggacat
     3361 gccggtcatt gtgctcctcg acaaaggagg tcaccggtcg ggacggggtc gacctgcccg
     3421 tcggcggcga tcggccgtcc gcgaagccac gtctggcgga cgactccgcg cagggtgcgc
     3481 ccggcgtacg gcgtgagcgg atgtcggtga tgcagcctgg ccgcgtcgac cacgaacgtc
     3541 tcgtccggag cgaacaccgc gaagtccgcg tcgtgcccga cgacgatccc gcccttgccg
     3601 gcgacgccga cgatcgcggc cggcgcggtg gccatccagg tcgcgacatc gtgcagggcg
     3661 tggccgcgct gcgccgcctg cgcccagacg acggacaggc cgagctggag ggaggcgatg
     3721 ccgccccagg ccgcgccgaa gtcgccgatg tcgggttgct tgagctcagg tgtcgcgggt
     3781 gagtggtccg acaccaccat gtcgacgaca ccgtcggcca gtccctgcca caaccgttcg
     3841 cggttgtcgt ggctgcggat cggcggacaa cacttgtacg ccgtcgcacc gtcctcgatc
     3901 agctcggcct cgagcgcgag gtagtgcggg caggtctcga cgctgatccg aacaccgtcg
     3961 gcctttgcac gcgccaaccg gtccagcgag ccggcgttcg acaggtgcac gacgtggaca
     4021 cgggcctcgt gctcgatcgc cgccgcgatc agggcggcga tggcgcggtc ctctgcctgg
     4081 ggagggcgcg aggccacgaa ggcggcgtac gagcagccga cgtccacggt cgccgcctcg
     4141 atggtggcgg cgtcttcggc atgcgcgatc agcaggccgt cgaacgccgc cacctcggcc
     4201 atcgccgcgt cgagctcggc gcggcccagc ggcgcgaact ccgccacacc gctgtcgagc
     4261 aggaagcact tgaacccgaa gacgcccgcg tcgtacagcg gcccgaggcc ccccaggttg
     4321 gtcggcacgg caccacccca gaatccgacg tcgacgtagc acttgccctc ggccgccgcc
     4381 cgcttgacct cgagcgccgg caggtcggtg gtcggtggga tcgagttcag cggcatgtcg
     4441 acgatggtgg tgacgccacc tgctgccgcg gcccgggtcg cggtctcgaa accctcccac
     4501 tcggtgcggc cgggctcgtt gacgtggacg tgggtgtcga cgagcccagg cagcagcact
     4561 tcgtcggcgc gaagatcgac gacggtcgcg tgcgggtccg gcgaccacgc ctcgagatcg
     4621 tggatcgcag tgatccgacc gtggtccacc acgaccgcgg cctcgcgctc tgcgtcgggc
     4681 agcacgcatc gccctcggaa gacggtgcgg ctcatccgac ccgccgggcc gccagccgac
     4741 gtacgtcgtc gacgacggac tcgtcgccga ccgatgccgc gtcgatcgca ccgcagtcca
     4801 ggccacgtac caggaacgtc gcgagggcct gggcggtcgc cggctcgtcg agatgctggc
     4861 cgggtccgtc tccgccgggc agtcccaggt accgatgcag ccgggcgagc acgtcgggaa
     4921 ggtcctcgag aaagaacagg tacgtcgcgg cgtaccggct cgggagctgc gccggatgga
     4981 ggtcccaacc ctggtagaac ccgcgctgca gcgaccggcg tacgagtcgt gcgtgctccg
     5041 accaggcacg gtggacctga tcggtgtcac cgaccggcag caggttggtg gagccgtcgg
     5101 acactcgcac agcggtttgc gcggcggcca gcgccatcac ctgcttggca tgctcggccg
     5161 ccggatggtc catcgcctgg tacgtggcgc tgactccgag ggcggccgtg tagtcgtacg
     5221 tcccgaagtg caaccccgcg cacctgccgt ctgccgtgtg cacgagccgc gccaccaggg
     5281 cggtgccgtc gtacgcgagg atcgactgcg ccgtctcgat ctggatctcg aagcggagcc
     5341 gatggtctgc gagcccgtac gccgactcga gtctcgcgca cgctgccacc atcgcctcga
     5401 cctgctcgac ggacgtgacc ttcggcaggg tcgcgacgaa acccgcgggc aggtcgcctc
     5461 cctcgagcag cgtgccgagg aacaggtcga gcgtgcgcaa cccacgtcgg cgagtgctgg
     5521 cctccaacgc cttgaagcgg atgccgcaga acggcagcgg ctgctccagg atcccggcct
     5581 gcgtcagcgc acgggccgcc gcgacggcat cgcggtcctc gatgtcgtcg tcgcggatcc
     5641 cgtagccgtc ctcgaggtcg atgcgcaggt cctcgatcgg ctctgtggcc agcttggcga
     5701 ggatgcggcc ccagccgtcg ttcacctcca gtgccgcagg ctgcgagaga cccagcgcag
     5761 ctgcgaactc cttgctggat gacgcgtgct cgtcgagggc tcgcctggct gccttccccc
     5821 aacgtgccgc aacgtcggcg gtcatccggt cggcgggcag gtagaccgtg tggaccggct
     5881 ggcggcctcc cccgtcgccg gggtacagcc gggccatccg ctcgtccgca ggagccacga
     5941 tcgcatcgag cgctgcgaga tcgtcctcgc cgagtcgtgg ctctcgatcc gtccgcctcc
     6001 gcgcggattc atccggcgtg gtcgcgccgg acgagtcggg gttgctcacg cgacgtactc
     6061 ctcgtatgcc ggcaacgtca ggaagtcggc gaagtcgtcg tcgagggcga cccgttcgaa
     6121 caacgtgcgg gcctggtcga gcgcttccat cgtggccggt tggtcggccc aggacgtgct
     6181 cagctcggca tacacctcct cgacggtggc ccggacgtgc tctgcggaga cgatcgtccc
     6241 gtcggccgtc ttgctgcctg catgggtcca ctgccacagc tggctgcgtg agatctcggc
     6301 cgtggccgcg tcctccatca ggttgtcgat ggcggcggcg ccggtcccga gcagccagga
     6361 caccaggtag cggatcgcca cggacacgtt gccgcgcacc ccggcatcgg tgatgtcgcc
     6421 ttcggtggcc gcgacgtcga gcaggtccgc ggccgagacc gacacgtcac gctgccggtc
     6481 gagctggttc ggccggtcgc cgaggatctc gtcgaagacc tccatgcaga ccggtacgag
     6541 gtcggggtgg gccacccagg agccgtcgta cccgccatcg gcctcgcgtt gcttgtcctc
     6601 ccggaccttc tgcagcgcct tctcgttgac ctcggcatca cgacggctcg ggatgaacgc
     6661 cgccatgccg cccatcgcga aggcaccgcg ccggtggcag gtggcgacga gcagctcgga
     6721 gtacgcctgc atgaacggct gtgtcatcgt caccgcggcg cggtcgggga gcacgaacga
     6781 ctcgcccgcg tcacggaagt tcttgatcac gctgaacagg tagtcccagc gccccgcatt
     6841 caggcccgac gcgtgctcct gcagctcgaa caggatctcc tccatctcga acgccgccgt
     6901 gatcgtctcg atcagcacgg tggcgcggat gctgccgtac gggatgccga ggacgtcttg
     6961 agcgtgcgtg aacacgtcgt tccacagtcg ggcctcgaga tggctctcca tcttcggcag
     7021 gtagtagtac gggccgaggc cgcgctggag cagctcctcg gcgttgtgga agaagtgcag
     7081 cccgaagtcg agcagcccgc cgaccatcgg ctgaccgtcg acgagcacat gtttctccgg
     7141 caggtgccaa cctcgtggcc ggacgacggg aacggcgagg tcaccgtcgt tcaacgcgta
     7201 ctccttgccg tccggtgacg tgaacaccag cacgtgtcgg tgcgcgtcgt acaacgacac
     7261 ctgaccttcg acgacgttgt tccagtgcgg ggtgttggcg tcctcgaagt cggcgagcca
     7321 gatcttggcg ccggagttca gcgcgttgat cgccatcttc gcgtccgtcg ggccggtgat
     7381 ctcgacccgc cggtcctgca gcgcgaccgg cgcgggcgcg acctgccact cggcctctct
     7441 gacgtcgcgg gtctcgtcga ggaagtccag ggtgccggtc tggctgatcg tcgtacgccg
     7501 ctcggctctt gcgcgcaaca gctcgtcgcg cctgccaccg aacatccgtt gcaggtcggc
     7561 cacgaacgcc agcgcctgcg gcgtcaggat ctcctcctgc cggggctcgt cctgcggcgc
     7621 cttgacctcg actccagcca gatcagtcat gcgccccatc gttgtccgct cttcccgcac
     7681 ccgcaacgca gcgccgccga ggtggcaatg gtgtgtcgac acgccggcag aaatgaacga
     7741 ttcctgccac ctcggcggga gtcagatgat gtgttcgggg cggacgggga tgcgggtgag
     7801 gtcgcggccg caggcctgtc ggatggcggc cagcaccgcg ggaccggacg agatcgtcgg
     7861 cgcctcaccg acaccgcgga cgccgtacgg cgcattgggg tcggcgtact ccagcacctc
     7921 gatcggcatc ggcggtgtgt cgaggatggt cgggatcagg tagtcggtga acgacgggtt
     7981 gcggatcttg ccgtcgacga tctggatctc ctccatcacc gccagcccca tgccctgcgt
     8041 gctgccgccc tggatctgac cgagcaccgc gtcggggttg atcgccttgc ccacgtcctg
     8101 cgtgcaggcg agctccacca ccttgaccag gccgagctcg acatcgacgt cgacgacagc
     8161 gcgatgcgcg gcgaaggcgt attgcacgtg ggcgaatccc tgcccggtct cgggatcgat
     8221 gggttcggtc ctgcggtgcc gccactcgag cgtctcctcg accacatcgg tgccgaggac
     8281 gtcggcgatc gaggccacca caccgccgtc cgacatcacc atgcccgcct cgaggcgcag
     8341 ctcgtgcagc gggcggcgca ggtagtcgcg ggccctctcg aagacctgct cgcgcacctt
     8401 ccggcacgcc gcctggacgg cgccgccggt gacgtaggtc tgtcgcgacg ccgacgtgga
     8461 gccacccgag cccacctggg tgtccgccgg ggccaccacc accgtgtcga cgccgagctc
     8521 cgtccggcag atctgctgct cgaccgtgac gagcccctgg cccacctcgg ccgatgcggt
     8581 gtgcaccgtg acgtgtggcg agccggcgat gatctcgagc cgcacccggg ccgtcgagta
     8641 gtcgtcgaaa ccctcggaga acgccacgtt cttgtacgcc accgcgtagc cgacgccccg
     8701 gcgtacacct tcaccatgcg tcgtgttgga gacgccgccc ggcaggtgcc gcagatcgct
     8761 cgggtcgaga tccggcggca gtggcatcgc cgtcagccgc tgcagcagct cggcgaccgg
     8821 ggcggcgctg tcgatcacct gcccggtcgg ggccttgctc ccctcctcga acgcgttcct
     8881 gagtcggaag tcgaccgggt ccatcccgag cgctgcagcg agcttgtcca tctgggcctc
     8941 gacggcgtac gccgtctgga ccgatccgaa gccccgcatc gcgccgcatg gtgggttgtt
     9001 cgtgtaggcg ccgaagcagt ccacctggat gttcgggacg acgtacgggc cgaggcccat
     9061 cgtcccggcg ttccccacca ccgcgggtgt cgaggaggca tacgcgccac cgtcgaggta
     9121 gatctgcgcc ttggcgtaga cgagcttgcc gtcccgggtt gcgccgtgct cgtagtgcat
     9181 gctcgccggg tgccggtgca catggccgaa gaacgactcc tcccggttgt agaccatctt
     9241 gaccggcttg ccggtgtgca tcgccagcat gcagccgtgc acgtgcatcg acaggtcttc
     9301 gcgtccaccg aaggcgccgc cgaccccggc cagcgacagg cgcacctggt ccgggcgcag
     9361 gccgagcgcg gaacagatct gcttctggtc gacgtgcaac cactgggtcg cgacgtacag
     9421 gtcgatgccg cccacgccgt cggggacagc cagtccggac tccggaccga ggaacgcctg
     9481 gtcctgcatc ccgacctcgt agtcgccggc cacgatgacg tcggcggtca cgtcggggtc
     9541 accgacgcgg accttcaggt gccgcaccag gttgccctgg gggtgcaggt cggcggactc
     9601 gcgactcagc tcggtcacct cacccgcgag gtcgccgtgc aacacgaccc ctggatggcc
     9661 gagcgcggtg cgggcatccg tcaccggttc cagtacgtcg tagtgcacga cgatcttctt
     9721 cgccgcctgc cgggcgatgt caggatggtc ggcggccacc agcgcgatcg gctcgccctg
     9781 gtagcggacg acgtcgatcg cgagcaccgg ttggtcggcc acctccagcc cgtacgtctt
     9841 gtggccgggc acgtcctcat gcgtcagcac cgcgaacact ccggcggtcg ccaacgccgg
     9901 gccgatctcg atgccggtga tgcgtgcgta cgggtgtggg ctgcgcagcg tgacgcccca
     9961 gagcatgtcg tccatccaca ggtcgctgcc gtaggcgaac tcgccggtga ccttcaaggt
    10021 cgcgtcgggc cgccgcgcac tctcgccgac gcccccgatc gtgttgtccc gcaacgagtc
    10081 gggcgccccc gtcggcgtct gcggcgtcgt cgtcggttcc gccgtactcc gttccccgct
    10141 cctggggtat ccgcgaattg cgggaaaatg cacgtctgag ttgaggtttt cacctgacct
    10201 ttcccgcaat tcgcggcagc cacgggtgac ggaagcgttg ggtggactgg tcatgacgtg
    10261 actcctgctc gggagagcaa cgctgtactg gcggcttgga cgtcccgggc gacagccgct
    10321 tcgtccaccg tgacgagctc gtcgtgtcgt acgacgggcg agccctgcac cagcaacagc
    10381 tccagcggcg gtgccgagcc caggacgagg gccgccaccg ggtcgtcgat gtcggcatgt
    10441 gcgatcgtgt ccagccgcca cagcgccagg tcggcgagct tgccggtctc gatcgaaccg
    10501 atctggtcgt cccagccgag cacctccgcg ccgccgatcg tcgcgagctc cagcgcctgc
    10561 cgaacgctca acgcagtcgg tccaccgcgc gcgcgggcga acagaacggc gtggcgcagc
    10621 tcctcgacga gcgaggccgc ttcgttcgac gcggcaccgt cgacgccgag tcccaccggt
    10681 acgccgacgt cgagcaggtc acgggtgcgg cagatgccgg cgccgagtct cgcgttcgac
    10741 gacgggcagt gcgcgacgcc ggtgcgcgtg tcagccagtc gctgcacggc ctcgtcgtcg
    10801 aagtggatgc cgtgggccaa ccagacgtcg ctgcccagcc agcccaacga ctcgacgtac
    10861 tcgaccggtg agcagccgaa cttctgcgcg cagaagtcgt cctcgtcgac ggtctccgcg
    10921 aggtgggtgt gcagccggac gcccttgtcg cgggcgagtc cggcggactc cttgagcagg
    10981 tcgccggtca ccgagaacgg cgagcacggt gccacaccga cccgcagcat cgagtccgga
    11041 ctcgggtcgt ggtgggtgtc gatcgcgtcg gccgtggcct gcaggaccgt gtcgatgtcc
    11101 tccacgacgt ggtcgggtgg caggccgcct tggctcttgc ccaggtccat cgagccgcgg
    11161 gtcggcagga accgcagacc gacctgccgg gccgcgtcga tctcggccgc cagcaggtcg
    11221 cctcctccgg cagggaagac gtagtgatga tccatcgtgg tggtgcagcc ggtgcgcgcg
    11281 agccaaccca gaccgccgag ggcggcgacg ttcaccgtcg cctcgtcgat gcacgcccag
    11341 acgggataca gcgtggtcaa ccactcgaac agcgagtggt cgacggccat gccccgggtg
    11401 acccactggt acaggtggtg gtgggtgttg accagcccgg gagtggccag gcagccggtg
    11461 gcgtcgacgt actcagcgtc gccgacgtcg cgtggcgctc gccccgcgcc gaccgaggag
    11521 atgcgggcac cctcgacgac cacgtgaccc actgagtact ccgtccgcgc agagtccatc
    11581 gtgacgaccg cacacccgtc cagcacgagg gcgttgctgc ggccggcgtt cacggcggac
    11641 caccgcgatc gactgccgtg gccattcggc cggccgccat gcggaccgcg tcgaggatct
    11701 tctcgtatcc cgtgcaccgg cacaggttcc ccgccagcgc ctctcggatc tcggcgtcgt
    11761 cgggttcggg gtcacgctgc agcaggtcat gggtctggac gatcaggccc ggcgtacaga
    11821 agccacactg caccgcaccc gcttcgatga acgcctgctg gatcgggtgc aggtcgtcaa
    11881 cctccgccaa gccctcgacc gtgacgatct cgcggtcttg cgcctggccc gctgcgacga
    11941 gacaggcaca gaccggaagg ccgtcgaggt agatcgtgca cgagccgcat tcgccttgct
    12001 cacaagcgtt cttggagccg ggcaagccca tccgctcgcg caacacgtag agaaggctct
    12061 cgccctccca cacgtcgtcg gcctggtgga tctcgccgtt gacggtggcg gtgatcctca
    12121 tcgcccgctc cttcccgggt cggcgtagtc ggcccacgcc catgccaacg tgcgtcgcgc
    12181 catcacggcg agcgcgtgca gccggtaggc cgccgtccct cggacgtcgt cgatcggcga
    12241 cgccgccgcc ttgacacgtt cgccgaactc ctgcgccacg gactccggga tctcgccgcg
    12301 cgactcccag agcgaccctt cggccagcgc gcccgcgagg aagtcctcgg cgtcccgtgc
    12361 acgtcgaggg gtgggcgccg cggatccgac gccggtacga atctgttgcc ggtcggggtg
    12421 gagcgcgatc gagaacgccg agaccgagat gaccatcgcg ttgcgggtgc cgatcttgct
    12481 gaactgttgc gggccggacg gcggcctgac cacgatggcg gtgatgagct cgtcgggccg
    12541 gccggcgtgc tgcttcacgc cggtgtagaa ctcgtcgatc ggtacccggc gtacgccgct
    12601 tgcactggcg agctcgacct cggtgtccgc gcagagcagt ggcgggtgcg agtcaccggc
    12661 cggcgaggcc gcgcccaggt tgccgcccac cgtgccgcgg ttgcggatct gcggcgagcc
    12721 cacggtccgc gacgccatcg ccagtcccgg caggatggcg ccgagctctt cgatgacctc
    12781 ggtgtacgtg acggcggccc cgagccggac gaagccgtcg tcgagctccc agtcggcgag
    12841 ctccggtatc cgggtcaggt cgagcagggc gggtggccgg tgccggtcga agttgagctc
    12901 gaccatgacg tcggtgccgc cgatgatcgg catcgcgtcg ggtatctcct ccttcgccgc
    12961 gagggcgtcc gccagcgtgg tcggcgctag gaactccatc gcgcctccca tgccgggccc
    13021 gcgtccggtg cgtcggctcg gctgaccgcc gcctggatca ggccgtacgg ccggtcgtcg
    13081 gcgtggaaca cctcgccgtc gttggtgagc ccgaaccgct cgaggtcgta ggcgaagtgg
    13141 tgcaggttcg gggccgacag ccgcacctcg acgacctgcg gctgccgctc gaggacggcc
    13201 gtgcccatct cgtacaacgt ctgttggagg gcgaggctgt gcacggtcgc gtagcgctca
    13261 agcaatgtgg cgcggatgtc gtcgtacgcc gcttgccagt tgccaggaac gaccgaaccg
    13321 gctgccagcg tgtcgtagcg ccaacggacc acgagcgagg tggccatcac ccggtcgtgt
    13381 gtctcggcca gcgtggtgaa ctcgtcggtg aggaagcccg cgaactccga gccggtggac
    13441 ttgagcacga cgaggtcctg gacccccgag acgacccaac cccgctggtc gtcgccgcgg
    13501 ccttcgacgg tgaccgccgt cgtccgcacg ccctcgccgg tccggacgaa gctgtggtcg
    13561 tggccgacgc cgtcgacctc gatgcggtcc cagccgaact cctcgatcgc caccctggcg
    13621 ccgctgaccg gtgcgacgtc gtcgacgaag tgcctggcga gtgccagccc gaagtcctcg
    13681 atctggtcga cgccgtgcga cttggcgaac gcgaagacgg tgttcttctg cgtgtcggtc
    13741 gggagcacct tcgactggtc gccctcggtg tacgccgccg cgaagtcccc gcgcagcgtc
    13801 gtggtcacgt tgaggtcgtg gatcacgtgg cgggacgagt cacggtcgac cttgaccagc
    13861 cggttctcgg ccttgccgta ctggtcctgc agcacgaacg tggccgggct ctggctgggg
    13921 ttgtcttccg ggtggatcgt catcagctgc cccggtacgt cgagtagccg aacgggctga
    13981 gcaggagcgg cacgtggtag tgcgagtcgt cggtcacctc gaagcagatg ctcacctccg
    14041 gatagaaggc cgccgctcct gctgtggcga agtacgcacc ggtgttgaag cgcagccggt
    14101 acgtcccggg gtcgagccgc tccggtccga ggtccgcgac gcggccgtcc tcgtcggtct
    14161 cgccggtcgc cactgcgggc ggctcggtcg cgctggcatt gtcgtgctcg agcaccaccg
    14221 tcaggcccac cgcagggcgt ccgtgcgcgg tgtcgaggac atgggtggtg acgtggctca
    14281 tggctgaacg acctgctgga gccgtagcac ggcgatctcg cgcaatgcgc agaccgtctc
    14341 cctgcgttcg gtttcgtcgt cgttgtcgag gcgccgctcg agctcgtcca ggatctgcgt
    14401 gccggaccgt ccggcggccc gcacgaggaa gacgcggccg aacctgtcct cgtagcggcg
    14461 attgcctttc tggagtcggt ccgcaagctc gggatggtca ctcacgccgg actgttcgcg
    14521 ccgtgagtga tcggcctcct ggccgccggc gacgtcgggg tcagcgcgct cgccgatccg
    14581 cggatgacgg gcgagcgcct ccgccagctc gccgtcggaa aggttctcgg cactggcctt
    14641 ggcctgcacg accagtgccg ccaggtcggc atacggtcgg cctgccacga cctcgtcgac
    14701 ccaacggcgt acgccgaggc agggatcgac cgtgcgttgc gcctcgtctg cagcgagtcc
    14761 gttgaactcc ctcagctgca tacgacttcc tcccgccagg cggtcgtgct tggcgcaaca
    14821 ccctaccggc gatgagcact gccaaccctt cgagtgatgg atcaccccta cgctgaacgg
    14881 gtgcggatcg tctcgctcct gccgtccacg tcggagatcc tgttcgcgct cggtgccggt
    14941 gactcggtcg tcggcgtgac gttcgagtgc gactaccctc ctctggcgcg gacccggcgc
    15001 atcgtgtcga ccagcagcct ccccgagggt ctggagccgg cagccatcga cgccgtcgtg
    15061 gcggagcggt tggccgccgg ggaggatctc taccacctcg acgagggcgc cctcgccgac
    15121 ctggatgcgg acatcgtcgt cacccaggac ctgtgcgcgg tgtgcgcggt ggacgtgtcc
    15181 gtcgtggacg acgcgctcgc tcatctcggc tgccgtgccg aggtggtcac gatcgacccg
    15241 cacacactcg acgaggtgct cgcatcgatc gatgtcctcg gcaaggtgtc gggcaccgag
    15301 cagcgggccg ccgaccttgt cgagtcgctg cgaacacggc tggcagccgt cgagtcgtcg
    15361 gtggccgggc tcccgcaacc gcggatgctg gtgctcgagt ggaccgagcc accgttcgcc
    15421 ccggggcact gggtgccaga gatgatcgag cgtgccggcg gcctgtcgac gctcggcgct
    15481 gccggcgaga agtcgacccg tacgacatgg gatgcggttg ccgccagtca gccggaggtg
    15541 atcgtgagcg cgccgtgcgg ctatgcgctc acgccgtcgg tcgatctggc cgaaaagctc
    15601 gtagggacgg gggtgctccc ggacggcgta cctctctggg cggtggacgc gaacgcgtcg
    15661 ttcgctcgtc ccggaccccg cctcatcgac ggcgtcgagg tcctggccgc gatcgcgcac
    15721 gccgaggcgt gcggtccccc cgatcccacc ctcgcgcacc agatccgctg accgtcgtca
    15781 caccgacgat gctcacaggt cgccggggaa caggttggcg tcggacggcc gcccggtgtg
    15841 caacacccac tcgcgccaga agctgcgcag gtccactccg gtgcgccgct cgagctggtt
    15901 gcgcagtccg atcatcgaga tgtgcccacc cgcgaggtcc cgctggatcc cccgcatcgc
    15961 acggaagaag ctcgcgtccc cgatcttgtt gcgcagcgcg tgcacgaagt acttgccctt
    16021 gacgtagacc ggcgtgtcga actcgtggtt ggcgcccatg tcgtagagct tgccgctgaa
    16081 gagactgtcg ccgatcctgg cgactctgat ccggtaccgg tggtcgaggt cgacaccgtt
    16141 gtgctcgtcc cacagccact cggagtacga cgcgagacac tcgttgaggc agatgtcacg
    16201 ccacctcttg atggacacgt tgtcgcccca ccactggtgg ccgttctcgt gcacgatcgt
    16261 gaggagaccg gtcgacctgg agtagatcgg acgagtgaac gtctcgagct cgtacggaat
    16321 gaaaccgctg acgaacatcc cgccggcctg tggagccggg tacgggcccc atcggcgtgc
    16381 cagcactcgc aggatctcgg gcagctgact ctcacgctcg ggcgcctccc caggattcgg
    16441 cccgtacgcc gagacgaccg gcgtcccgtc acgcagcgtc gagcgctcga acgtcagctt
    16501 gccgatgtac atccccgtca tgtacgtcgc ggtgcgctca ccgagccgcc aacgaaatgt
    16561 ccggaccgct acgccgtgcc gagtgcccga ggtggtgggg ccctgttcgc cgaccgacac
    16621 gaggtcgagc ggccgaggaa gggtggcggc caacgaccaa cgcgccttgt cgatcgggtg
    16681 gtcgttgcac gggtaccaca ccgcgcagcc gtgcggctca ccctccagaa caccgctgcc
    16741 ctccttgccg acagggatcc acccgctggg ggcgccgccg tcgatgacgt agccgagctt
    16801 gccgtggtag gcgacctccg tcacgaaacc cgccagcttg tgcaccggtc gagccggtgt
    16861 cacgacgagc tcgtgctgac cggttcgcct ccaggtcgcg gcgcggtcgt cgacggcgac
    16921 ggagtccacg tgcagacctt tcaggtccag gtggaaggac gggagtctcg tcgtggcgat
    16981 cgcgtgaacc caagtggtgg cgcggatgga ctcaggttgt gggaaatagt cgagccgaac
    17041 gtggtagcgg cggacgttgt agccggggtt gccgtcggcc gggtagtacg ggtcgccgat
    17101 tccgttcggg ttctccgttg ccacggcgac agcgtcgtgc gtcgtcgcgt ggcgcgaagg
    17161 tgtgcccgtg accccgacgg cgccgagggt cgggagaagg gttgcggtgg tgagagcggc
    17221 gatgccgagc aggcgacggt tcatggttcc cccgaccggg acgaggtgag gacggcggcc
    17281 gcggcgtacc gcctcgccga tcttcgcaca ggtcgtgcac cgacgggacc ggccggtagg
    17341 acctgttgaa acctccgatc agctgcctgc atcggcgggc tcgatcaccg cgccgtcaca
    17401 gatcgcccgg gtagaggttg gcgtcggagg ggcggccggt atcgatgacc cactcctgcc
    17461 agaagctggt gaggtcgacg ccggtacgtc gttcgagctc gtcgcgtagc ccgagcatcg
    17521 agaggttgcc gcccgagtcg tcgcgttgga tcccttgcag cgccgagaag aatgcctcgt
    17581 ccccgagctt gttgcgcagt gcgtgcagga agaacgtgcc cttgaagtag acgccgtagt
    17641 agtcgaacag gtgggccggg cccatgtcgt acagcggcag gtcgaaccag tcggcaccgc
    17701 cacggtcgac ggcctcgtgg taccgcgcgt ccaggtcagc cccgtgcgcc tcgtcccaca
    17761 gccagacgga gtacgacgcg aagcactcgt tcaggcagat gtcgcgccac cgcatgaccg
    17821 acacgttgtc gccccaccac tgatgggcgt tctcgtgcac gatcgtcgcg acgtcggcgc
    17881 cgtacgtgta gagcggccgg gtgtaggtct ccagcgagaa cggcacctct gcggtcacga
    17941 agatgccgcc cgctgcaggt gcggggtagg ggccccaggc gtcgctcagc acccggagga
    18001 tctccggcag cttcgcctcc cgctgcggcg ccccgcgggg atgcgggccg tacgcggaca
    18061 cgaccggggt cccgtccccg agcgtcgatc gctcgaacgt gagcttgtcg atgtagagcg
    18121 tggtcaggta cgtcgctgtc ggctcgtcca gtcgccatcg gtaggtccgc gccttcgtac
    18181 cgtctcgaag cgttgtcgcc gtggcgactc cttggacgcc gaccgagacg acctcgagcg
    18241 gtctcgggac cgtggcggtg agggcgaagg tcgccttgtc ggtcgggtga tcgttgcagg
    18301 ggtaccacac cgtgcacgag tgcggttcgc cggcgatgaa gccgccgcct ggcgtgacgg
    18361 cgtcgtacca cccgctctgc accggaccga ccggatcggc accgggccga ccgtggtagc
    18421 cgacgaccgt cgtgaaccgg cgcgtctgct gtatcggcgt gcgcggtgtg atgacgagct
    18481 cgtgggcgtc ggtgcgacgc tgccgcgcgg gcttgccgtc gactcgtgcg tcgtcgacgt
    18541 gcaggcccaa caggtcgagg tggaagctcc gcagccgctg cgtcgctctt gcgatgatcg
    18601 tcgtccgcgc cgtgatggcg tggtgcgcag ggtcgtaggt gaggcgcacg tggtagccga
    18661 tgacgtcgta cccgaggttg ccgtcgtcgg ggaagtacgg gtcgccgacg ccctgaccgc
    18721 ttgcgggcga cgagccgctc gcggtctcgg cgtcatccga cgtgcacgcc gtcgtgacca
    18781 aggccaacgc agcgagcgcc acgacggcga cggtctgctt cgtgtgacgc actcgcatgg
    18841 agcccctcgc tgccgcgccg gtgacggctg ccgcgatcat cgcaccaccg gcacaccagc
    18901 ggaaccgact gcagccgtct tgtgacctgc cacgtggaga gcacggatgg cttggggcgg
    18961 tgggggtttg cctggatagc catccgaatg cacggctacc caggcaaacc ggcggtccgc
    19021 ctgcatctgt tccgtgggcc gagacggtcg agtttggtgg acgtggtcgt ccggccctcc
    19081 ctggctcgtg gctgaggtgc gatggcagcg aagagcgggg agcgttcctg cccggcgagc
    19141 tcgccggctt tccgttcctt tttgttcggt atgtgagtgg cgggtttcgg acattcgtcg
    19201 gtcgcgtggc tcaccgagcc tcggtttgcc tggctaccaa ggcacctcca cccctcccaa
    19261 ggacggtttg cctgggtagc catccgaatg cacggctacc gaggcaaacc ggggtccgcc
    19321 tctggttccg tgggcggaga cggtcgcgat ggttggcgtg atcgcccgag gtgccgtcgg
    19381 cctcggctga gctggttggc agggaagcgc ggacgcgttc gtgcccggcg agcctcgttg
    19441 ccggctttcc gtcccctttg ttcggtatgt gaatggcggg tttcggacat ccgacactga
    19501 ccggcccgag tttgcggtgt ctacatccga aaccccctgt gcccatcgaa ggtttgcgat
    19561 gttcacacgc cttttgggct gtaaacatcg caaacctcaa ccgcgcagct cgcctgccgt
    19621 agggcttgga gaccggtga
//
LOCUS       DATBFZ010000338         6524 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_1868085, whole genome shotgun sequence.
ACCESSION   DATBFZ010000338 DATBFZ010000000
VERSION     DATBFZ010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 6524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6524
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1868085"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..880
                     /locus_tag="VH419_10475"
     CDS             <1..880
                     /locus_tag="VH419_10475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044080.1"
                     /translation="EPTGRPVILEIKLARNSEARRAVVAQALSYAAYLKGLDVDTLEQ
                     GPLRRHLAEREFSGLVEACQAGDQADSTPASAIRSALEDSLSTGTFRIVLLLDDAPYE
                     LVRLVGYLESLSDRLTIDLVTVTAYDVGGSRVLVPQRVEPDRVIEETPTRAPTHRAEG
                     DLTPGSAMFRKAIEDSPPEHRAELTRLSDWADRLGERGLATIQSYRGSTGRFTLLPRL
                     PGGGGFVTIWNDGGPSLSLWRTVFEKRGPRSMERVEELIAPVRIGQGNIIREPSDETL
                     EALTQAYVEAAERRLP"
     gene            complement(885..1487)
                     /locus_tag="VH419_10480"
     CDS             complement(885..1487)
                     /locus_tag="VH419_10480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044081.1"
                     /translation="MARTRMRGDRVCVGGLEVETARSVRLMGSDGWNLREDHPIRPGE
                     IWDLTYRNSPDPMPPHVEDVVVAYGRRVSTVTDVRSELEAIVDPWRGPVGAIFDGRLR
                     TNVNGRAYVGPQPPLPSGSVGFWVTEYPLSFSRDAAVQGRRYWFPEGHAVTSIAYVGM
                     EPPLEVIPVGALVRFSLARWAAFPQGSEERCYLQVSGWYL"
     gene            complement(1499..1978)
                     /locus_tag="VH419_10485"
     CDS             complement(1499..1978)
                     /locus_tag="VH419_10485"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HEY7044082.1"
                     /translation="MADVLTVGAFGWSEAGFFDALAEARVGLFCDIRRRRGVRGAEYA
                     FVNSARLQGRLEDLGIPYVHRLDLAPSEHVRRVQAEADEREGIAKRSRSVLGDAFVTA
                     YTAEHLTDFDARGFLDETASTRPVCLFCVEREPAACHRSLLAAALENAGASVEHLTP"
     gene            complement(1994..2554)
                     /locus_tag="VH419_10490"
     CDS             complement(1994..2554)
                     /locus_tag="VH419_10490"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HEY7044083.1"
                     /translation="MEAQSPIRTIGYGARSIDELVSALGSKGTDYLVDVRSAPYSRFK
                     PEFSKEPLAARLEREGVSYIFMGDALGGRPGDPSCYDADGRVDYERCRLRPAFQDGVA
                     SLEAGWEGGHRIVLMCSEGKPQECHRTKLVAEELVRMGVPVVHIDEQGVERSHEAVME
                     SITNGQGTLFGASAAAAKSRKKHRAA"
     gene            2973..6326
                     /locus_tag="VH419_10495"
     CDS             2973..6326
                     /locus_tag="VH419_10495"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEY7044084.1"
                     /translation="MIRGAMADFARLTPRTRLSRTCTAVDAGHPSSKSLSATSGLILR
                     SCIHASLWVSTYSPHVRRSTPCWPRLVMAEGPLFIVDNSEGGRNGLDYLREWSELASS
                     MDIATGYFEIGSLLDLDGHWQKLDKIRILMGDEVTRRTKKAMLLALRQRAEAHLDESI
                     EDHKDRNPFLVGIDAIVAALESGQIECRVYNRDKFHAKTYITYGRFDVIGSQALVGSS
                     NFTRPGLTQNVELNIKIESSSEVAQLQEWYEQHWNDAVDVSPDVVRVIERHARPFSPF
                     EVYAQALRSMFGELEPTADDWDQHHSKMFPKLDRYQQEAYWSMVNIARQHGGALLCDG
                     VGLGKTYVGLLLVERLVLHENKRVVLFAPKSVKDSVWVPELKKHLPHIGGVDGSADFS
                     NLSVFSHTDLTRALEYPERFERITELADAVVIDEAHHFRNTGKRGDPGDPFTRSRYYK
                     LYDLIGEARHKSVFMLTATPINNSLNDFKHLVELFSRGDDAYFSRTLGVTSLGGRLNS
                     ITKTLKEQLGDEVPEAEAAEEAADLLRGDDLFQGLVVQRSRAYARASQIQETGNSAAF
                     PERDDPRVAEYSIRKSYGKLLDLVDTAFQKAKPLFALPMYYPLAYYTGPDTSIDPLEW
                     NRQAQVVGLIRTNFLKRFESSVYAFELSCDRLLRKLLAFVDVNSESPAERKRYERWLD
                     QHQEMLTQARVRQLTLWGEEEEPDEENEDIVPPELLERAHHLARDEYDVPEILAETYL
                     DLDQIATLLDESRSFEPSHDDKLKKLVRLLKAKDLKDRKVLIFTEFADTARYLRRELQ
                     KAGIDGVAELDSNTKVNRADAIRRFSPYYNGLSSVELAATGREETQVLIATDVLSEGL
                     NLQDATRVINYDIHWNPVRLMQRIGRVDRRLNPDVEASLVADHPDLAKERGRIAFWNF
                     LPPDDLDTLLLLYGKVSHKTLLISKTLGIEGKKLLKPDDDYEALKEFNSSYEGETTIQ
                     EQLHLEYQQLLDEHDGLRERLAGLPTAIFSGRQRTTEGSEGVFFCYRLPALDTESGDF
                     TLEAGVTHWYLHILRHDTLLDTPKQIADAIRSSPDTPRSCAVERPLLVTIRDGVLKHI
                     KNTYLKQLDVPIGAPKPQLVCWMELNAAP"
     gene            6323..>6524
                     /locus_tag="VH419_10500"
     CDS             6323..>6524
                     /locus_tag="VH419_10500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044085.1"
                     /translation="MTNLDPNHLRDITSFEDVIEYLADELDWPIEAADLEDATFEWDP
                     DELGIPAERVPHLASLRQLRPLE"
BASE COUNT         1224 a         2183 c         2069 g         1048 t
ORIGIN      
        1 cgagccgacg ggtcggccgg tgatcctcga gatcaagctg gcacgaaaca gcgaggctcg
       61 cagggcggtc gtggctcagg cgctctccta cgccgcgtat ttgaaggggc tcgacgtcga
      121 cacgctcgag cagggcccgc ttcgccggca tcttgccgag cgggagttct ccgggctggt
      181 ggaggcgtgc caggcgggcg accaggccga tagcacgcca gcgagcgcga tccgctccgc
      241 gttggaggac tccctctcca cgggcacgtt ccggattgtg ctcctcctgg acgacgcgcc
      301 gtacgaactc gtccgcttgg tcggctacct ggagagcctg tccgatcggc tcacgatcga
      361 cctcgtcacg gtgaccgcgt acgacgtcgg gggatcgcga gtgctcgttc cgcagcgggt
      421 ggagccggat cgagtgatcg aggagacgcc aacgcgggcg ccgacgcatc gagcggaggg
      481 cgatctgacg ccgggctccg cgatgttccg gaaggcgatc gaggactcgc cgccagagca
      541 ccgcgccgag ctcacccgtc tcagcgattg ggcggaccgc ctgggtgagc gcgggctggc
      601 aacgatccag agctaccgcg gatcgacggg ccgcttcacg ctcctcccac ggctgcccgg
      661 cgggggcgga ttcgtcacca tctggaacga cggcggcccg tcgttgtcgc tctggcgcac
      721 ggtgttcgag aagcgcggtc cgcggagcat ggagcgcgtg gaggagctca tcgcgccggt
      781 ccggatcggt caggggaaca tcatccgcga gccgagcgac gagacgttgg aggcgctgac
      841 ccaggcgtac gtcgaagcgg ccgagagacg tctgccgtag gcgcctagag gtaccacccg
      901 gaaacctgga gatagcagcg ctcctcggac ccttgcggga acgccgccca ccgcgccagc
      961 gagaatcgga cgagtgcccc gacggggatg acttcgagcg gtggctccat cccgacatac
     1021 gcaatcgacg tgacggcgtg gccttccggg aaccagtacc gccttccctg caccgcagca
     1081 tcgcgcgaga acgagagtgg gtactcggtg acccagaacc cgacgctccc acttggcagt
     1141 ggcggctgcg ggccgacgta cgccctcccg ttcacgttcg tccgtagccg gccgtcgaaa
     1201 atcgcaccga caggccctcg ccacggatcg acgatcgcct cgagctccga gcgcacgtct
     1261 gtcacggtgc tgacgcgacg gccgtacgcg acgaccacgt cttcgacgtg cggaggcatg
     1321 ggatcgggag agttccggta cgtgaggtcc cagatctcgc cgggtcggat cgggtgatct
     1381 tcgcggaggt tccatccgtc gcttcccatg agccggacgc ttcgggcggt ctccacctcg
     1441 agcccgccga cgcagacccg gtcgcctcgc atccgagtgc gtgccacgac gaggacgttc
     1501 atggagtcag gtgctcgacg ctggctccgg cgttctcgag ggcggctgcg agcagcgagc
     1561 ggtggcaggc cgcaggctca cgctcgacgc agaagaggca caccggacgg gtcgacgccg
     1621 tctcatcgag gaagcctcgg gcgtcgaagt cggtgaggtg ctcggccgtg taggcggtga
     1681 cgaaagcgtc gccgagtacc gaccgcgacc gcttggcgat gccctcgcgc tcgtcggcct
     1741 cggcctgcac ccgccggacg tgctcgctcg gcgcgaggtc gagccggtgg acgtaaggga
     1801 tgccgagatc ctccagccgg ccctgcaggc gggcgctgtt gacgaacgcg tactcggctc
     1861 cgcggacacc ccgacggcgg cggatgtcgc agaagaggcc aacgcgagcc tccgcgaggg
     1921 cgtcgaagaa ccccgcctcg ctccatccga acgcaccgac ggtgagaacg tctgccatct
     1981 cgtgaacgcc gtcctacgcg gcgcggtgct tcttacgcga cttcgcagcc gccgcgcttg
     2041 ctccgaagag cgtgccctgc ccgttggtga tcgattccat taccgcctcg tgcgatcgct
     2101 ccacgccctg ttcgtcgatg tgcaccaccg ggactcccat ccgaacgagc tcctccgcga
     2161 cgagcttcgt ccggtggcac tcttgcggct tgccctcgct gcacatgagg acgatgcgat
     2221 gcccgccctc ccagccggcc tcgagcgatg ccaccccgtc ttggaacgcg ggccgcagcc
     2281 ggcagcgctc gtagtccacg cggccgtcgg cgtcgtagca gctggggtcg cccggtcgcc
     2341 cgccgagggc gtcgcccatg aagatgtagc tgacgccctc gcgctcgagc cgggccgcga
     2401 gcggctcctt ggagaattcg ggcttgaagc gggagtatgg ggctgagcgg acgtcaacca
     2461 ggtagtcggt ccctttcgaa cccagggccg agacgagctc gtcgatcgag cgggcaccgt
     2521 agccgatggt gcgaatcggg ctctgtgcct ccatggcgac cactctagcg gtcgggtacg
     2581 acgagtgtca cgagccttcg cgttccacgt gaacctggaa cggataacca agctcgacac
     2641 ggagaagttc atgggccgga tggccctgcg cgcaagagtt gaggccggga tcacccaccg
     2701 gatggcagga ctggcggctc gtcccggcga tcgtggatcg gtatccgatc cgcggttgat
     2761 cccgcggaag ccgaagccaa cgagccgcgc gtgcgcggtc atcgacagga cgtaaaacag
     2821 ggcgggcgag gcccgacgac ccgtcggggg aaaatggggg gtctctccga cgcccgagac
     2881 cgttgccgct gtacccttgc ccgcgatgcg actacgcgga acgggcgtcg tcatggcgga
     2941 tgcctccgcc ggcccctact cgccgccgga cggtgatccg cggcgcgatg gcagactttg
     3001 cacggctaac gccgcgtaca cgactatcgc gaacctgtac cgccgtagac gccgggcacc
     3061 cgagctcaaa gagcctgtcg gcgacctctg gcctgattct tcgatcctgt attcatgcaa
     3121 gcctctgggt ctcgacgtac agcccgcacg tccgtcgatc tactccctgt tggccgaggc
     3181 tagtcatggc tgaggggccg ctcttcatag tggacaacag cgaaggtggg cgcaatggcc
     3241 tcgactacct tcgcgagtgg tcggagttgg cgagcagcat ggacatcgca acgggctatt
     3301 tcgaaatcgg ctcgctgctc gatctcgatg gacactggca gaagctcgac aagatccgga
     3361 tcctgatggg cgatgaggtc acccgccgga caaagaaggc gatgctgctg gccctcaggc
     3421 agcgcgccga agctcacctg gacgagagca ttgaagacca caaggacagg aacccgttcc
     3481 ttgtcggaat cgacgccatc gtcgccgccc tcgagtcggg ccagatcgag tgccgggtct
     3541 acaaccggga caaattccac gcgaagacgt acatcactta cggccggttc gacgtaatcg
     3601 gctcccaggc gctggtcggg tcaagcaact tcacccgtcc ggggctcacc cagaacgtcg
     3661 agctgaacat caagatcgag agcagttccg aggtcgctca gctccaggag tggtatgaac
     3721 agcactggaa cgacgccgtc gacgtctcac ccgatgtcgt gagagtgatc gagcggcacg
     3781 ctcggccgtt cagcccgttc gaggtctacg cacaggccct gcggtcaatg ttcggcgagc
     3841 tggaacccac ggcggacgac tgggaccagc accactcaaa gatgttccca aagctcgacc
     3901 ggtaccagca ggaggcgtac tggtcgatgg tcaacatcgc ccgacagcat gggggagcgc
     3961 tcctctgcga cggcgtcggt ctgggaaaga cctacgtcgg actcttactc gtcgaacgcc
     4021 tcgtgctcca cgagaacaaa cgggtggtgc tcttcgcccc caagtcggtg aaggattcgg
     4081 tgtgggtacc cgaactgaag aagcaccttc cgcacatcgg cggagtcgac ggctcggccg
     4141 acttctcgaa cctatcggtg tttagccaca cagacctgac ccgagcgctg gagtaccccg
     4201 agcgctttga gcggataacc gagttggcag atgcggtcgt gatcgacgag gctcaccact
     4261 tccgcaacac cggtaagcgt ggcgatccgg gggacccgtt caccagatcg cgctactaca
     4321 agctgtacga cctaatcggc gaggcccgtc acaagagcgt gttcatgctg acggcgaccc
     4381 cgatcaacaa ctcgctcaac gacttcaagc acctcgtcga actgttcagc cgaggcgacg
     4441 acgcctactt ctcccgcaca ctcggcgtga cgagcctggg cggccgattg aactcgatca
     4501 ccaagaccct caaggagcag ctcggcgacg aggtgcccga ggccgaggct gccgaggaag
     4561 cagcggacct cctcaggggt gacgacctgt tccagggcct cgtggtccag cggagtcggg
     4621 cctacgcccg agcgagccag atccaagaga cagggaacag tgccgcgttc cccgagcggg
     4681 acgatccaag ggtcgccgag tactcgatcc ggaagagcta cggcaagctc ctggatttgg
     4741 tcgacaccgc gttccagaag gccaagccgc tgttcgcatt gccgatgtac tacccgcttg
     4801 cctactacac cgggccggac acgtcgatcg acccgctgga atggaaccgc caggctcagg
     4861 tcgtcggact catccgcacg aacttcttga agcggtttga gagctccgtg tacgcgttcg
     4921 agctgtcgtg cgaccgcctc cttcgcaagc tgctcgcatt cgtcgacgtt aacagcgagt
     4981 cacctgccga gcgaaaacgc tacgagcggt ggctcgacca gcaccaagag atgctaacgc
     5041 aggcccgtgt ccggcagctg acgctgtggg gtgaggagga ggagcctgac gaggagaacg
     5101 aggacatcgt cccacctgag ctgttggaaa gggcccacca cctggcccgc gatgagtacg
     5161 acgtacccga gatcctggcc gaaacctacc ttgacctaga tcagatcgcc acactgctgg
     5221 acgagtcacg ctcgttcgag ccgtcccacg acgacaagct caagaagctt gtgcggctcc
     5281 tcaaggccaa ggacctaaag gatcgcaagg tcctcatctt cactgagttc gccgataccg
     5341 cgcgctacct gcgtcgggag cttcagaagg ccggaatcga tggcgtcgcc gagctggaca
     5401 gcaacacaaa ggtcaaccgg gccgacgcca tccgccgctt ctcgccttac tacaacggcc
     5461 tctcgtccgt cgaactggcc gccaccggcc gcgaggagac acaggtcctc atcgccaccg
     5521 atgtcctttc ggaaggtctg aacctccagg acgccacgcg cgtcatcaac tacgacatcc
     5581 actggaaccc ggtccggctc atgcagcgca tcggtcgtgt cgaccgccgc ctcaacccgg
     5641 acgtcgaggc cagcctcgtg gccgaccacc ccgacctcgc caaggagcgg ggacgcatcg
     5701 ccttctggaa cttcctgcct cctgacgacc tcgacaccct gctgctgctc tacggcaaag
     5761 tgagccataa gacgctgctc atctcgaaga cactcggcat cgagggcaag aagttgctca
     5821 agcctgacga cgactatgaa gcactcaaag agttcaactc atcctacgaa ggcgagacga
     5881 cgatccaaga gcagctccac ctggagtacc agcagttgct cgacgagcac gacggcctca
     5941 gggagcgtct cgcgggccta ccaaccgcga tcttcagcgg acgccagcgc actactgagg
     6001 gtagcgaagg cgtgttcttc tgctatcgcc tgcccgcgct cgacaccgag tccggggact
     6061 tcactctgga ggccggcgtc acccactggt acttgcacat cctccgtcac gacacgttgc
     6121 tcgacacacc caagcagatc gccgacgcaa tccggtcgtc gcctgacaca ccgcggtctt
     6181 gcgccgtcga gcgtccgctg ctcgtgacca tccgcgacgg cgtgctcaag cacatcaaga
     6241 acacctacct gaaacagctg gatgtgccga tcggagcacc caagccccaa ctcgtgtgct
     6301 ggatggagct aaacgccgcc ccatgaccaa tctggacccg aatcacctcc gcgacatcac
     6361 gtccttcgag gatgttatcg agtacctcgc cgacgagctc gactggccca tcgaggccgc
     6421 tgatctcgag gatgccacct tcgagtggga ccccgacgag ctcggtatcc ccgcggagcg
     6481 ggtacctcac ctagcctcgc ttcggcagct ccgcccactt gaga
//
LOCUS       DATBFZ010000339         1909 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_6352120, whole genome shotgun sequence.
ACCESSION   DATBFZ010000339 DATBFZ010000000
VERSION     DATBFZ010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6352120"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="VH419_10505"
     CDS             complement(<1..656)
                     /locus_tag="VH419_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017936699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY7044086.1"
                     /translation="MLGSASDAEDVVQETWIRWDAIGAAARDEVRDPRAFLVRMTTRK
                     ALDRLRANARRREEYVGEWLPEPLLTAPDVAEDVELAESVSFAMLTVLETLTPTERAV
                     FVLREVFDLSYGEIAEAVDKSAAAVRQIGHRAREHVAARRPRMEVSRREQEEVVERFL
                     AAVTGGDLQGLLDALAPDVVLVADGGGIAQAIPNPVRGAKKVANLLRTFPKFGGGATI
                     L"
     gene            complement(716..1300)
                     /locus_tag="VH419_10510"
     CDS             complement(716..1300)
                     /locus_tag="VH419_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEY7044087.1"
                     /translation="MTGTTRIPAAEITGFKGWLIKRFARKALGQVPSSIGVYWHNKSV
                     LMDMAAAGGKVKKWDACDEQLKSFAHMAVASRVGCTWCLDFNYFEAHNKNLDITKARE
                     IPRWREADVFSPLEREVLAYAEAMTETEPTVTDEMVSSLRAQLGDAALVELTAVIAFA
                     NFTTRPNVALGIESDGFAAACGLKPMAPRVAVGS"
     gene            complement(1322..1468)
                     /locus_tag="VH419_10515"
     CDS             complement(1322..1468)
                     /locus_tag="VH419_10515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044088.1"
                     /translation="MALRAYDALGTSYELWVIDAGTHDVLDPAQVRIGSRCHTWEPGG
                     VLMS"
     gene            1573..>1909
                     /locus_tag="VH419_10520"
     CDS             1573..>1909
                     /locus_tag="VH419_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEY7044089.1"
                     /translation="MANRLANATSPYLLQHADNPVDWWEWGPEAFDEARRRNVPVLLS
                     VGYAACHWCHVMAHESFEDEATAAYLNEHFVPVKVDREERPDVDAVYMQATTAMTGQG
                     GWPMTCVLDH"
BASE COUNT          299 a          669 c          643 g          298 t
ORIGIN      
        1 aggatggtcg cgccgccgcc gaacttcggg aacgtccgca gcaggttggc gaccttcttc
       61 gcacccctca cggggttggg gatggcctga gcgatcccgc caccgtcggc caccaggacg
      121 acgtccggcg cgagtgcgtc cagcaggccc tgcaggtcac cgccggtcac cgcggcgagg
      181 aaccgttcga cgacctcctc ctgctcgcga cggctcacct ccatccgggg tcgccgggcc
      241 gcgacgtgct cccgagcccg gtgcccgatc tgacgtacgg cggccgcaga cttgtcgacc
      301 gcctccgcga tctcgccgta cgacaggtcg aacacctcgc gcagcacgaa gaccgcgcgc
      361 tcggtcggcg tgagggtctc gagcacggtg agcatcgcaa acgacacgct ctccgccagc
      421 tcgacgtcct ccgcgacgtc cggcgccgtc agcagcggct cgggcagcca ctcgccgacg
      481 tactcctcac gccggcgcgc gttcgcccgc agccggtcga gcgccttgcg cgtggtcatc
      541 cggaccagga acgcgcgcgg gtcgcggacc tcgtcgcgag ccgcggcgcc gatggcgtcc
      601 cagcggatcc aggtctcctg cacgacgtcc tcggcatcgg aagccgagcc gagcatctcg
      661 taggcaacgg tgaacagcag acctttgtgg ctgacgaacg gatcctcggt cacggtcacg
      721 accctacggc gacccgtggc gccatcggct tcaggccgca cgcggcagcg aagccgtcgg
      781 actcgatgcc gagcgcgacg ttcggacggg tcgtgaagtt ggcgaacgcg atcaccgcgg
      841 tgagctcgac caacgcggcg tcgccgagct gcgcccgaag cgagctgacc atctcgtcgg
      901 tcaccgtcgg ctcggtctcg gtcatggcct cggcgtaggc gagcacctcc cgctccaaag
      961 gcgagaagac gtcggcctcg cgccagcggg ggatctcgcg cgccttggtg atgtcgaggt
     1021 tcttgttgtg ggcctcgaag tagttgaagt ccaggcacca cgtgcagccg acccgcgagg
     1081 cgaccgccat gtgcgcgaac gacttgagct gctcgtcaca ggcgtcccac ttcttgacct
     1141 tgcccccggc ggcggccatg tccatgagca ccgacttgtt gtgccagtag accccgatcg
     1201 agctcggcac ctggccgagc gccttccgcg cgaagcgctt gatcaaccag cccttgaacc
     1261 cggtgatctc ggcggcgggg atacgagtgg ttccggtcat gtcgtcctcc tgtcgtgggt
     1321 cttacgacat caagacgccg cccggctccc aggtgtgaca tcgggacccg atccggacct
     1381 gcgcggggtc caagacgtca tgcgttccgg catctatcac ccacagctcg tacgacgtcc
     1441 cgagggcgtc atacgctcgc agcgccatga ccgccccctt gtacgacgtt ccgctgcagg
     1501 aggcagggcg agacccgttg cgggtcactc aggtcgttcg agctcgatga gtgcccggct
     1561 agcgtgggga gcgtggccaa ccgccttgcc aatgcgacca gtccctacct gctgcagcac
     1621 gccgacaacc ccgttgactg gtgggagtgg ggaccggagg cgtttgacga ggcgcggcgt
     1681 cgcaatgtgc cggtgctcct gagcgtcgga tacgccgcct gccactggtg ccacgtgatg
     1741 gcgcacgagt cgttcgagga cgaggcgacg gcggcgtacc tcaacgagca cttcgtgcct
     1801 gtcaaggtcg accgcgagga gcggccggac gtggacgcgg tctacatgca ggcgacgacg
     1861 gcgatgaccg ggcagggcgg ctggccgatg acgtgcgtgc tcgaccacg
//
LOCUS       DATBFZ010000340         2550 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_3183276, whole genome shotgun sequence.
ACCESSION   DATBFZ010000340 DATBFZ010000000
VERSION     DATBFZ010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2550
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3183276"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..889)
                     /locus_tag="VH419_10525"
     CDS             complement(95..889)
                     /locus_tag="VH419_10525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044090.1"
                     /translation="MTEQRADLRRYDDWLAWLRRQGETDADVRVVFIGGSAVTGGYDD
                     HSDLDVEVLTTPGQAVATYRRLLDTALRDFEVVHVWELPESTYPDGRQAFLNLTSDAG
                     DMSRPTRIIDLHVSDLADEHRLVDERRHGEPFVVHDPDGLVELRHDDEETMARARAEA
                     VQQAAARRPTAQWLVNRAIARGDEVEAHAFHTGYGVNPLVRLLRIKHCPARHDFGLRY
                     LRTDLPAGYAERVAALLDGGDLQTRADATFAWQDELLGEMAASSGV"
     gene            1097..>2550
                     /gene="leuS"
                     /locus_tag="VH419_10530"
     CDS             1097..>2550
                     /gene="leuS"
                     /locus_tag="VH419_10530"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017935600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEY7044091.1"
                     /translation="MSQEHATPGGATDGGAATYDITAVEEKWYRVWEDLQPFKASDDV
                     VQSGTREKRYALTMFPYPSGDLHMGHAEVFALHDVVARYWWQRGYEVLNPMGFDSFGL
                     NAENAAIKENAHPATFTYANIAISTESCRRFGASFDWTRTLNTSDPEYYRWTQWLFLK
                     FYERGLAYRKKSPVNWCPHDQTVLANEQVIDGRCERCGAEVTKRELTQWYFKITDYAQ
                     ELLDCLDDLAPTWPDRVITAQRNWIGRSEGAHVRFPVVSTGSTDGAGSTDGAGSTDEH
                     PPIEVYTTRPDTLFGATFMVVAADAALAGELVSDEQRPAYEAYLEEVRKSSEIDRLAT
                     DRPKTGVFLGVRATNPVTGEQIPVYAADYVLADYGTGAIMAVPGQDQRDWDFAKVFDL
                     PIVRTVQPPDDFDGEAYTGDGPAINSQWLDGLPVAEAKARAIQWLEEQGLGEGTVQFR
                     LRDWLLSRQRFWGCPIPVVYCDTDGIVPVPDDQLP"
BASE COUNT          408 a          880 c          899 g          363 t
ORIGIN      
        1 cgatcgtcgt ggacgaggag cagctcgaga aggacaagcg ggaggtcgag gaggaggcgc
       61 accggcggct ggagggcggt tcgaccacct gacgtcagac gcctgatgag gcggccatct
      121 cgccgagcag ctcgtcctgc cacgcgaacg tcgcgtcggc ccgggtctgc aggtcgcctc
      181 cgtcgagcag cgccgccacc cgctcggcgt accccgccgg caggtcggtg cgcaggtacc
      241 gcagaccgaa gtcatggcgc gcggggcagt gcttgatgcg cagcaggcgg accagcgggt
      301 tcacgccgta gccggtgtgg aaggcgtgcg cctccacctc gtcaccgcgg gcgatcgccc
      361 ggttgaccag ccactgggcc gtcggccggc gagcggccgc ctgctggacg gcctcggcac
      421 gggccctggc catggtctcc tcgtcgtcgt ggcgcagctc gaccaggccg tcggggtcgt
      481 ggacgacgaa cggctctccg tgcctgcgtt cgtcgaccag gcgatgctcg tccgccaggt
      541 cggagacgtg gaggtcgatg atcctggtcg gccgtgacat gtcacctgcg tcggacgtga
      601 ggttgaggaa cgcctgccga ccgtcggggt acgtcgactc cggcagctcc cagacgtgca
      661 ccacctcgaa gtcgcgcagt gcggtgtcga gcagccggcg gtacgtcgca accgcctggc
      721 ccggcgtcgt caggacctcg acgtcgaggt ccgagtggtc gtcgtaccca cccgtgacgg
      781 ccgacccgcc gatgaagacg acccgcacgt ccgcgtcggt ctcaccctgc cggcgcagcc
      841 aggccagcca gtcgtcgtac ctgcgcaggt cggcgcgctg ttcggtcatg cccgggaagg
      901 taggggcaac cgacgggcgg tcgccagcac gtttcggtta cgatggtcgc atgctgcacc
      961 accggagcac cctgcttctt cgacagccgc gttgacgctc tcgcgtcgcg cggccgcccc
     1021 tccctgcgag gggctttttc atgcccaggg aacccgtgag agtgtcggag cgcagaagcg
     1081 agacggagac gtgacgatga gccaggaaca tgccacaccc ggcggtgcca ccgacggagg
     1141 cgccgcgacg tacgacatca cggccgtcga ggagaagtgg taccgggtct gggaggacct
     1201 gcagccgttc aaggcgagcg acgacgtggt gcagtccggc acgcgcgaga agcgctacgc
     1261 gctgacgatg ttcccctacc cgtcgggcga cctgcacatg ggtcacgccg aggtgttcgc
     1321 tctgcacgac gtcgtcgcgc gctactggtg gcagcgcggc tacgaggtgc tgaaccccat
     1381 gggcttcgac tcgttcggcc tgaacgccga gaacgccgcg atcaaggaga acgcgcatcc
     1441 ggccacgttc acctacgcca acatcgcgat ctcgacagag tcgtgccggc gcttcggggc
     1501 gagcttcgac tggacgcgca cgctgaacac cagcgacccg gagtactacc ggtggacaca
     1561 gtggctgttc ctgaagttct acgagcgcgg cctggcctac cggaagaaga gcccggtcaa
     1621 ctggtgcccg cacgaccaga ccgtgctggc caacgagcag gtgatcgacg gacgctgcga
     1681 gcggtgcgga gccgaggtca ccaagcgtga gctgacccag tggtacttca agatcaccga
     1741 ctacgcccag gagctgctgg actgcctgga cgacctggcc ccgacctggc ccgaccgcgt
     1801 gatcaccgcc cagcggaact ggatcgggcg gtccgagggc gcgcacgtgc gcttcccggt
     1861 ggtctcgaca ggctcgaccg acggggccgg ctcgaccgac ggggccggct cgaccgacga
     1921 gcatccgccc atcgaggtgt acaccacccg tcccgacacc ctgttcggtg cgacgttcat
     1981 ggtggtcgcg gccgacgccg cgctggctgg tgagctggtc agcgacgagc agcgcccggc
     2041 gtacgaggcc tacctggagg aggtgcgcaa gtccagcgag atcgaccgcc tggccaccga
     2101 ccggccgaag accggcgtct tcctgggcgt ccgtgcgacc aacccggtca ccggcgagca
     2161 gattcccgtg tacgccgcgg actacgtgct ggccgactac ggcaccggtg cgatcatggc
     2221 cgtgcccggc caggaccagc gcgactggga cttcgcgaag gtcttcgacc tgccgatcgt
     2281 gcgcaccgtc cagccgcccg acgacttcga cggcgaggcc tacacgggcg acggcccggc
     2341 catcaacagc cagtggctgg acggcctgcc ggtcgccgag gccaaggcca gggccatcca
     2401 gtggctggag gagcagggcc tcggcgaggg cacggtccag ttccgcctgc gggactggct
     2461 gctgagccgc cagcggttct ggggctgccc gatcccggtc gtctactgcg acaccgacgg
     2521 catcgtgccg gtgcccgacg accagctgcc
//
LOCUS       DATBFZ010000341         5162 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_2523108, whole genome shotgun sequence.
ACCESSION   DATBFZ010000341 DATBFZ010000000
VERSION     DATBFZ010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 5162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5162
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2523108"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="VH419_10535"
     CDS             complement(<1..408)
                     /locus_tag="VH419_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012920872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEY7044092.1"
                     /translation="MPDPSLYRPTPGSIPDAPGVYRFRDPNGRVIYVGKAKSLRARLS
                     SYFQNPANLHERTARMVTTAGSVEWTVVQTEVEALQLEYSWIKEFDPRFNVKYRDDKS
                     YPWLAITLNEEFPRVMVGRGPKKKGVRYFGPYSH"
     gene            730..2019
                     /locus_tag="VH419_10540"
     CDS             730..2019
                     /locus_tag="VH419_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008357180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HEY7044093.1"
                     /translation="MPQVSDLYGLRLTTSSVASDTYNRGVGALLRVQEGALSAISESV
                     THDPTFALGHAGLALLGHEYCAAVDVDARVAAAVRHLDRADARERSHVHAVAAHIRGD
                     SRPMIRHLREYPRDALLMSVAVPTIAFAGVTKVPQESWAIVERARPAYGDDWWYAGLL
                     GFIRQEQRRWDEAMSLACRSLDVEPAAGHSVHARTHVHYETGDHEAGRDWLDGWINGP
                     GVTADNLAHYSWHAALHELSMGDFEAVQRRYTAELAPPTVFGPRALVDSCSLLWRWAI
                     TPGARDVPDLDAVVGVIDDEMLDHPPTPFMALHSAVTMCALGEVGRLERLEAWAARHQ
                     DTTYAEVVSPLCDALRALASGDPSTAADRLTHMLGMIWRLGGSDAQREVVEDTLIASL
                     LAAGRYDEARPVIDRRMDRRTCRRDEAFLAATLAGSS"
     gene            complement(2119..2652)
                     /locus_tag="VH419_10545"
     CDS             complement(2119..2652)
                     /locus_tag="VH419_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017935234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HEY7044094.1"
                     /translation="MTDVWSELDRDGKDPAPAGSTRRAVLRGALVAGALPLLAACGGG
                     DSTDASSTSGGVGNGGGNGADPTSADPPGGGNGGAGTAVQPIAQTTDVPEGGGIILDD
                     PGVVITQPDPNDFKGFSNICTHMHCPVDNVSDGTINCVCHGSQFSITDGSVVAGPAPT
                     PLPAMQLKVQGKNISLA"
     gene            complement(2814..>5162)
                     /gene="uvrA"
                     /locus_tag="VH419_10550"
     CDS             complement(2814..>5162)
                     /gene="uvrA"
                     /locus_tag="VH419_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013161392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEY7044095.1"
                     /translation="DDDPHRERVFSEHLACMYDDLSFEELEPRSFSFNSPFGACSDCH
                     GLGTRMVVDPELIVPDGRKSLDDGAIAPWSAAHVSDYFGRLLDALADEMGFSTRTPWQ
                     QLPKKAHEAVLNGVSRKLHVRYKNRYGRERSYYAQFEGVIPYIERRHGEADSDISRER
                     FEGFMREVPCLSCDGARLKPISLAVTVGGKNIAEICAMPVDEASAFLRDDLDMSPREK
                     QIAERVIKEINERLRFLLDVGLDYLTLNRPTGSLSGGEAQRIRLATQIGAGLVGVLYV
                     LDEPSIGLHQRDNHRLIDTLVRLKDLGNTLIVVEHDEDTIRTADWAVDIGPGAGEHGG
                     QIVVSGPVQDLLDHPDSLTGAYLSGREEIPLPVVRRPPTPGRVLTVHEAREHNLHDVT
                     VSFPLGCFIAITGVSGSGKSTLVNTILYTALAKRLYNAREVPGRHRDITGVEHVDKVI
                     HVDQSPIGRTPRSNPATYTGVFDHIRRLFASTAEAKMRGYLQGRFSFNVKGGRCEACS
                     GDGTIKIEMNFLPDVYVPCEVCHGARYNRETLEVHYKGRTIAEVLDMPIEEAVEFFEA
                     IPPISRHLRTLVDVGLGYVRLGQPAPTLSGGEAQRVKLASELQKRSTGRTVYVLDEPT
                     TGLHFEDIRKLLGVLQRLVDQGNTVMVIEHNLDVIKTADWLIDLGPEGGNKGGLVVAE
                     GTPEYVAALPASYTGAFLRPILDGRAVGVARRPRKTKATAAATGTAVTTAKATKTTAK
                     TTTKARAAQTTTKKAAPRKTAAKKTAKQAATKRATKRSTTRN"
BASE COUNT          761 a         1715 c         1787 g          899 t
ORIGIN      
        1 gtgcgagtac ggcccgaagt agcgcactcc cttcttcttc gggcccctcc ccaccatcac
       61 gcgggggaac tcctcgttca acgtgatcgc cagccacggg taggacttgt cgtcgcggta
      121 cttcacgttg aagcgcgggt cgaactcttt gatccaggag tactcgagct gcagcgcctc
      181 gacctccgtc tggacgacgg tccactcgac cgagccggcc gtggtgacca tccgggcggt
      241 gcgttcgtgc aggttcgccg gattctggaa gtacgacgac agccgggccc gcagcgactt
      301 ggccttgccg acgtagatga cgcgaccgtt cggatccctg aaccggtaca cccctggggc
      361 gtccgggatc gacccgggcg tcgggcgata cagggaggga tcgggcacgg ctccacccta
      421 cgaggaaccg ccaaccgttc tcgctgccgg aaccccctgc cccgcccttg cctccccgcc
      481 gctgtgggct tccgcgaatt gcgggaaagt gcacgtcgac acgccgtcgc acccatgaca
      541 tttcccgcaa ttcgcggcag ccaggcagtc tgcggaggcc gggggtacgg cgtttgtggg
      601 tgatttcctg catggcctcg gtgcggttct tcgtcgatac tgcctgtacg gcgcgctgaa
      661 tgactgacgt gttagtccat cgcgccagta gactttatga ggcagccgcg acggggaagg
      721 aggcagccca tgccgcaggt cagcgacctc tacggcctcc ggctgaccac ctcgtccgtc
      781 gcgagcgaca cgtacaaccg cggagtcggc gcgctgcttc gggtgcagga gggggcgctc
      841 agcgcgatct ccgagtcggt gacgcacgac ccgacgttcg cgctcgggca tgccggcctc
      901 gccctgctcg ggcacgagta ctgtgccgcc gtcgacgtcg atgcccgggt ggcggcggca
      961 gtgcgtcacc tcgaccgggc cgacgcacga gagcgcagcc acgtgcacgc cgtggcggcc
     1021 cacatccgcg gcgactcccg accgatgatc cggcatctgc gcgagtaccc gcgcgatgca
     1081 ctgctgatgt ccgtcgccgt tccgacgatc gcgttcgccg gcgtgaccaa ggtcccgcag
     1141 gaatcgtggg cgatcgtcga acgcgcccgg ccggcatacg gggacgactg gtggtacgcc
     1201 ggtctgctcg gcttcatccg tcaagagcag cgacgttggg acgaggcgat gtcgttggcc
     1261 tgtcggtccc tggacgtgga gcccgccgcc gggcactcgg tgcatgctcg aacccacgtc
     1321 cactacgaga ccggtgacca cgaggcggga cgggactggc tggacgggtg gatcaacgga
     1381 cccggcgtga ctgccgacaa tctggcccac tactcgtggc atgcggcgct gcacgaactg
     1441 tcgatgggtg acttcgaggc ggtgcagcgc cggtacaccg ccgagcttgc tcccccaacc
     1501 gtgttcgggc cgcgtgccct cgtcgactcc tgctcgctgc tgtggcggtg ggcgatcacg
     1561 ccgggcgcgc gcgacgtacc ggacctcgat gcggtcgtcg gtgtgatcga cgacgagatg
     1621 ctcgaccacc caccgacacc cttcatggcg ctgcactccg ccgtcacgat gtgcgcgctc
     1681 ggcgaggtcg gccgactcga gcggctggag gcctgggctg cccgacacca ggacacgacg
     1741 tacgccgagg tggtctcacc cctgtgtgac gcactgcgcg cgctggcgtc cggtgacccg
     1801 agcaccgccg cggaccggct gacccacatg ctgggaatga tctggcggct cggcggcagc
     1861 gacgcccagc gtgaggtggt cgaggacacg ctgatcgcgt ccctgctggc tgccggtcgg
     1921 tatgacgagg ccaggccggt catcgaccgt cgaatggacc gacgcacatg tcgccgcgac
     1981 gaggccttcc tcgccgccac cctggccggc tcctcatagc gccctgcgcc ctgccctcca
     2041 gggcatgccc cgaaatgcgg gaagttccgg gatttcgcga cgcgaaaacc cggaacctcc
     2101 cgcaatcgcg ggggaacctc aggccagcga gatgttcttg ccttgcacct tcagctgcat
     2161 ggcgggcaag ggtgtgggag cgggtccggc cacgacactg ccatcagtga tcgagaactg
     2221 gctgccatgg cagacgcagt tgatcgtgcc gtcggacacg ttgtcgacgg ggcaatgcat
     2281 gtgcgtgcag atgttgctga accccttgaa gtcgttcggg tccggctggg tgatgacgac
     2341 accgggatcg tccaggatga tgccgccgcc ctcgggtacg tcggtcgtct gtgcgatcgg
     2401 ctggaccgcg gtgcctgcgc caccgttgcc tcccccgggc ggatcggcgg acgtcggatc
     2461 agcgccgttg cccccgccgt tgcccacgcc gccgctcgtg ctgctggcgt cagtgctgtc
     2521 accgccaccg caggcagcga gcagcggaag cgcgccggcg acaagggcgc cacggagcac
     2581 cgcacgccgg gtgctcccgg ccggcgctgg atccttcccg tctcggtcga gttcgctcca
     2641 cacgtcggtc atcgcagtct cctgagggca gggctgacac agctctggct ggtctggctg
     2701 gtctggcttg gtggtgctgc ctgctggtcc acacgttgtt cgtgcgcctg gttcgagcgt
     2761 aaccagccgg ccgccccgat ccatctgtcc aatggtccta ggaccaccgg ggactagttg
     2821 cgagtggtcg aacgcttcgt ggcacgcttc gtcgctgcct gcttcgcggt cttcttggcg
     2881 gccgtcttcc ggggagccgc cttcttggtc gtcgtctgcg cggcacgcgc cttggtcgtc
     2941 gtcttggcgg tcgtcttggt cgccttggcg gtcgtcaccg cggtgccggt tgctgctgca
     3001 gttgccttgg tcttccgggg tcgcctggcc acgcccaccg cgcgaccgtc gaggatcggt
     3061 cgcaggaacg ccccggtgta gctcgcgggc agtgcggcca cgtactcggg cgtgccttcg
     3121 gcgacgacca ggccgccctt gttgccgccc tccgggccga ggtcgatgag ccagtcggcg
     3181 gtcttgatga cgtcgaggtt gtgctcgatc accatgaccg tgttgccctg gtcgacgagc
     3241 cgctggagga caccgagcaa cttgcggatg tcctcgaagt gcagtccggt cgtcggctcg
     3301 tcgaggacgt acacggtccg gccggtcgaa cgcttctgga gctcggatgc gagcttcaca
     3361 cgttgcgcct cgccaccgga cagcgtagga gccggctggc cgagtcggac gtagccgagg
     3421 ccgacgtcga ccagcgtgcg caggtgccgg gagatcggcg ggatcgcctc gaagaactca
     3481 accgcctcct cgatcggcat gtcgaggacc tctgcgatcg tcctgccctt gtagtgcacc
     3541 tcgagcgtct cgcggttgta tcgcgctccg tgacagacct cgcacggcac gtagacgtcc
     3601 ggcaggaagt tcatctcgat cttgatcgtg ccgtcaccgg agcacgcctc gcagcggccg
     3661 cccttgacgt tgaacgagaa cctgccctgc aggtagccac gcatcttcgc ctctgccgtg
     3721 gatgcgaaga ggcggcggat gtggtcgaag acgccggtgt acgtcgccgg gttggagcgc
     3781 ggcgtacggc cgatgggcga ctggtcgacg tggatgacct tgtcgacgtg ctcgacaccc
     3841 gtgatgtcgc gatgccgccc gggaacctcg cgagcgttgt agagccgctt ggcgagcgcg
     3901 gtgtagagga tggtgttgac cagcgtcgac ttgcccgaac ccgagacgcc ggtgatcgcg
     3961 atgaagcagc cgagcgggaa cgagaccgtg acgtcgtgca ggttgtgctc gcgggcttcg
     4021 tggaccgtga gcacacgacc cggtgtcggc ggtcggcgga cgacgggcag cgggatctcc
     4081 tcgcgcccgg acaagtacgc gccggtgagc gagtcggggt ggtcgagcag gtcctgcacc
     4141 ggaccggaca cgacgatctg tccgccgtgc tcccccgcgc cggggccgat gtccacggcc
     4201 cagtcggcgg tgcggatcgt gtcctcgtcg tgttcgacga cgatcagcgt gttgccgagg
     4261 tccttcagcc ggaccagggt gtcgatcagc cggtggttgt cacgctggtg taacccgatc
     4321 gaaggctcgt caaggacgta gagcacaccc acgagcccgg caccgatctg ggtggccagg
     4381 cggatgcgct gcgcctcgcc gccggacagg gagccggtgg gccggttgag cgtgaggtag
     4441 tcgagtccga cgtcgagcag gaaccggagg cgttcgttga tctccttgat gacgcgctcg
     4501 gcgatctgct tctcgcgtgg cgacatgtcg aggtcgtcgc gcaggaacgc ggaggcctcg
     4561 tcgaccggca tcgcgcagat ctcggcgatg ttcttgccac cgaccgtcac cgcaagcgag
     4621 atcggcttca gccgggcgcc gtcgcacgag agacagggga cttcgcgcat gaacccctcg
     4681 aagcgctccc ggctgatgtc gctgtcggct tctccgtgcc gacgttcgat gtaggggatc
     4741 acgccctcga actgggcgta gtaggagcgt tcgcgcccgt agcggttctt gtagcggacg
     4801 tgcagcttgc gggacacgcc gttgaggaca gcctcgtgcg ccttcttcgg cagctgttgc
     4861 cagggggtgc gcgtcgagaa gcccatttcg tcggccagcg cgtcgagcag tcgaccgaag
     4921 tagtcggaga cgtgcgccgc cgaccatgga gcgatcgcgc cgtcgtcgag tgacttccgg
     4981 ccgtccggca cgatgagctc cgggtcgacg accatccgcg taccgagccc gtgacagtcg
     5041 ctgcacgccc cgaacggcga gttgaaggag aacgaccgcg gctcgagctc ctcgaacgag
     5101 aggtcgtcgt acatgcacgc gaggtgctcg gaaaagaccc gctcccggtg cgggtcgtca
     5161 tc
//
LOCUS       DATBFZ010000342         3098 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_2204365, whole genome shotgun sequence.
ACCESSION   DATBFZ010000342 DATBFZ010000000
VERSION     DATBFZ010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3098
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2204365"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1520
                     /locus_tag="VH419_10555"
     CDS             <1..1520
                     /locus_tag="VH419_10555"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012920944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HEY7044096.1"
                     /translation="AGFASIDPSDLRNRFRWWGLYTQRRPGIDGGRTAVLEPHELDDE
                     YFMLRVRIDGGQLTTQQLRTIADISTTYARDTADITDRQNIQLHWVRVEDVPDIWHRL
                     ESVGLSTQEACGDAPRVIIASPLAGVAADELIDPTPAVREIFDHYIGSPEFSNLPRKF
                     KTAITGHPSLDVAPEVNDVAFVPSVHPANGVGFDVWVGGGLSTNPQLAKSLGVWVPLH
                     EVADVWRGVISLFRDYGYRRLRSRARLKFLVADWGVERFRQVLEEQYLHRSLVDGEPP
                     ARPLGNGDHVGVHEQVDGRRYVGVSPVAGRISGTTLGKLADIVDAHGSNRVRTTPYQK
                     LVILDVERSAVDSLVAAVEDIGLSTRPSPWRRTTMACTGIEFCKLGIVETKARAAALV
                     EELEQRIPELDTPITIHLNGCPNSCARIQVADIGLKGQLVPDGNGDHVEGFQVHLGGG
                     LGLEAGFGRKLRAHKVTSTELTDYVERVVRNYLAGREPGERFAAWAARADEEELR"
     gene            1517..1747
                     /locus_tag="VH419_10560"
     CDS             1517..1747
                     /locus_tag="VH419_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008357238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044097.1"
                     /translation="MSTSTTHAASGSTHAVPLHCPYCAGEDLWPHGDVHGAWACRECL
                     RIFSVKFVGLSTPTPSNPAKTTLDRDDRRSTR"
     gene            1744..2463
                     /locus_tag="VH419_10565"
     CDS             1744..2463
                     /locus_tag="VH419_10565"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015068939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HEY7044098.1"
                     /translation="MTVTPTLGTARTSEQLRETALLAGPELEAAPAEAILEWAVQTFG
                     DRFCVASSMSDAVLSHMASQVAPGIDVLFLDTGYHFVETIGTRDAVAATLPVNVVTLS
                     PTISVREQDEQYGPDLWARDPDLCCNLRKVRPLSEGLTAYDAWATGLRREETRNRVMT
                     PVVGWDESKGKVRIAPLARWSQDDVDRYIAENDILVNPLQYDGYPSIGCWPCTHRVAP
                     GDDARSGRWSGTGKTECGIHS"
     gene            2460..>3098
                     /locus_tag="VH419_10570"
     CDS             2460..>3098
                     /locus_tag="VH419_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CbiX/SirB N-terminal domain-containing protein"
                     /protein_id="HEY7044099.1"
                     /translation="MSAPALIALAHGSRDARSARTVRDIVATTKAMRPDLKIEPAFLD
                     HSRPELSAVVDRLVARGHSEIVVVPLLLTEAYHARVDVPDVVHGVAERHEGLAIRVAD
                     VVGTDAALLSVLDERLREALRVSRVCELDALVLAAAGSSDALANTTVARLARLWGNRH
                     HLPTSVAFASTAPPSTGEAVRDWRRQGRRHVAVGSFFIAPGKLPDRAEELALE"
BASE COUNT          497 a         1081 c         1052 g          468 t
ORIGIN      
        1 gcgctgggtt cgcgtccatc gatccctccg acctgcgcaa ccggttccgc tggtggggcc
       61 tgtacaccca gcggcggccg ggcatcgacg gtggccggac cgccgtgctc gagccgcacg
      121 agctcgacga cgagtacttc atgctgcggg tccggatcga cggcggccag ctcacgacgc
      181 agcagctgcg cacgatcgcc gacatctcca cgacgtacgc ccgcgacacc gccgacatca
      241 ccgaccggca gaacatccag ctgcactggg tccgggtgga ggacgtgcct gacatctggc
      301 accggctcga gtccgtgggg ttgtcgacgc aggaggcctg tggagacgcg ccgcgcgtga
      361 tcatcgcgag cccgctcgcc ggcgtcgctg ccgacgagct gatcgacccc accccggcgg
      421 ttcgggagat cttcgatcac tacatcggtt cgccggagtt ctccaacctc ccacgcaagt
      481 tcaagaccgc gatcaccgga caccccagcc tggacgtggc cccggaggtc aacgacgtcg
      541 cgttcgtgcc gagcgtgcac cccgcaaacg gtgtgggttt cgacgtgtgg gtcggcggcg
      601 ggctctcgac gaaccctcaa ctggccaagt cgctgggggt ctgggtgccg ctccacgagg
      661 tggccgacgt gtggcgaggc gtcatcagcc tcttccgcga ctacggctac cggcggctgc
      721 ggtcccgggc aaggctgaag ttccttgtcg cggactgggg cgtggagcgg ttccgtcagg
      781 tgctggagga gcagtacctg caccggtcac tcgtcgacgg tgagccgccg gctcgaccgc
      841 tcggcaacgg tgaccacgtg ggcgtccacg aacaagtcga cgggcgccgg tacgtcggcg
      901 tctcccccgt cgccggccgc atctctggta cgacgctcgg caagctcgcg gacatcgtcg
      961 atgcccacgg ctccaacagg gtccggacga cgccgtacca gaagctggtg atcctcgacg
     1021 tcgaacgctc ggcggtcgac tccctcgtcg cagcagtcga agacatcgga ctgtcgacgc
     1081 ggccgagccc gtggcggcgt acgaccatgg cgtgcaccgg aatcgagttc tgcaagctcg
     1141 gcatcgtcga gaccaaggcg cgggccgccg cgctcgtcga ggagctggaa cagcgcatcc
     1201 cggagctcga cacgcccatc acgatccatc tgaacggctg cccgaactcc tgcgcccgta
     1261 tccaggtcgc cgacatcgga ctcaagggcc agctcgtccc cgacgggaac ggcgaccacg
     1321 tcgagggttt ccaggtgcac ctcggcggcg ggctcggcct cgaagcaggg ttcggccgca
     1381 agcttcgtgc ccacaaggtc acgtcgacgg agctcaccga ctacgtcgag cgcgtcgtcc
     1441 gcaactacct cgcaggtcgc gagccgggtg agcgattcgc cgcgtgggcg gcccgggcgg
     1501 acgaggagga gctgcgatga gcacgtcaac gacgcacgcc gcgtcaggct ccacccacgc
     1561 cgtgccgttg cactgcccgt actgcgccgg tgaggacctc tggccacatg gcgacgtcca
     1621 cggcgcgtgg gcctgccgcg agtgcctgcg gatcttcagc gtgaagttcg tgggtctgtc
     1681 gaccccgaca ccgtcgaatc ctgcaaagac gaccctcgac cgagacgatc gaaggagcac
     1741 ccgatgaccg tcactcccac gctcggcacg gcacgcacga gcgaacagct ccgagagacc
     1801 gccctgctcg cgggtcccga gctcgaggca gcgccggcgg aagccatcct cgagtgggcg
     1861 gtccagacgt tcggtgaccg gttctgcgtg gcgtcgtcga tgagtgacgc agtgttgtcg
     1921 cacatggcgt cgcaggtcgc cccgggcatc gacgtgctgt tcctcgacac gggatatcac
     1981 ttcgtcgaga cgatcggcac ccgggacgcg gtcgctgcga cgttaccggt caacgtcgtg
     2041 acactgtcgc cgacgatctc ggtgcgggaa caagacgagc agtacggccc cgacctgtgg
     2101 gcgcgtgatc ccgacctgtg ctgcaacctc cgcaaggtgc ggccgctgtc cgagggcctc
     2161 acggcgtacg acgcctgggc caccggactg cgtcgggaag aaacgcgcaa ccgggtgatg
     2221 acgcctgtcg tcggctggga cgagagcaag ggcaaggtgc gtatcgctcc actcgcccgc
     2281 tggtcgcagg acgacgtcga ccgctacatc gccgagaacg acatcctggt caaccccctg
     2341 cagtacgacg gctacccctc gatcggatgc tggccatgca cgcaccgcgt ggctccgggc
     2401 gacgatgccc gcagcggacg gtggtcgggt accggcaaga cagaatgcgg aatccactca
     2461 tgagcgctcc tgcactgatc gccctcgccc acggcagccg cgatgcccgg tcggcccgaa
     2521 ccgttcgcga catcgtcgcc accacgaagg cgatgcgacc cgacctgaag atcgaaccgg
     2581 cgttcctcga ccactcgcga cccgagctga gcgcggtcgt cgaccggctg gtggcacgcg
     2641 gccactccga gatcgtggtc gtcccgctgc tgttgaccga ggcgtaccac gccagggtcg
     2701 acgttcccga cgtcgtacac ggtgtggccg aacgccacga ggggctcgcg attcgggttg
     2761 ccgacgtcgt cgggacggac gccgcactgc tctccgtgct ggacgagcgg ctgcgtgagg
     2821 cgttgcgcgt gtcacgggtg tgcgagctcg atgcgctcgt gctggccgct gccggctcgt
     2881 ccgacgcact cgccaacacg acggtggcca gactggccag gctgtggggc aaccgccacc
     2941 acctcccgac cagcgtggcg ttcgcgtcca cggcgccgcc gtcgaccggt gaggcggtac
     3001 gcgactggcg tcggcagggg cgacggcacg tggctgtcgg gtcgttcttc atcgcgccgg
     3061 ggaagcttcc cgaccgcgcc gaggagcttg ctctcgag
//
LOCUS       DATBFZ010000343         8472 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_1874876, whole genome shotgun sequence.
ACCESSION   DATBFZ010000343 DATBFZ010000000
VERSION     DATBFZ010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 8472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8472
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1874876"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(226..2670)
                     /gene="pcrA"
                     /locus_tag="VH419_10575"
     CDS             complement(226..2670)
                     /gene="pcrA"
                     /locus_tag="VH419_10575"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HEY7044100.1"
                     /translation="MSALIPLPPLVDPPRSGEPVEDPDAEGRPRRRRLTAEDLLEGLN
                     ASQREAVTHEGTALLVVAGAGSGKTRVLTRRIAWLLSQRNVHPGSVLAITFTNKAAAE
                     MRERVEELVGGRAKIMWVSTFHSACVRILRREVSRLGYGSNFSIYDEADARRLMTLVC
                     RDLDLDLKRYAPRSILNWVSNCKNELLNPDAAAAKASNHLERTFAECYTTYQTRLRAA
                     NALDFDDLIMTTVQLFQGYPEVRETYRRRFRHVLVDEYQDTNHAQYVLVRELCGHGVG
                     RRTIDGDLVGAGSGGMSGEGPDEHAAEPAELMVVGDADQSIYAFRGASIRNILAFEED
                     FPNATTVLLEQNYRSTQTILSAANAVISRNKSRKPKNLWSDAGDGEQIVGYVADNEHD
                     EAQFVVEEIDRLTDEGLAHPSDVAVFYRTNAQSRVFEEVFIRVGLPYKVVGGVRFYER
                     REVRDALAYLRMLVNPDDTVSLRRILNVPKRGIGERAEACVEMLAERTGASYWEALRK
                     AEDAPELATRSLKNIQAFVAMIEDFQTMVEAGTRPDEVLEAVLTRSGYLAELEASDDP
                     QDETRVENLGELVAVAREFADEIDEATLGDFLERVALVADSDQIPDEDDSGGVVTLMT
                     LHTAKGLEFPVVFLTGLEDGIFPHSRAVGDVAELEEERRLAYVGLTRAKQRLYVSRAV
                     MRSAWGAPQHNPSSRFLAELPETLIDWKRTEADLTSWFSSSASTRYADRSGYIPAAAT
                     VQRRRASAAPAPQPTKGSRPVPSLEPGDRVVHDTFGMGSVVTVEGVAEKAVASIDFGS
                     LGVKRLLLRYSPVEKL"
     gene            complement(2831..3688)
                     /locus_tag="VH419_10580"
     CDS             complement(2831..3688)
                     /locus_tag="VH419_10580"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEY7044101.1"
                     /translation="MPSHRAEKHRATRGSVGRTRRAARAVPAGGRRRAPKAPRRSLGV
                     SLPSMVGGGALLAAAIGAATAGQAVSSSGTVSTASHQRGMYVANLSSASLEGRDATRV
                     SRAGDRPNITVTKPALQNAAERQAKARVALLRSAASDAQQYARELKSDQWVLPTSGFH
                     ISVWFGEAGGYWESGYHTGIDFATAYGTPAVAVANAKVAQTGWDGPYGNQVRLQFENG
                     DQVWYNHLSAIEVTKGETVLKGQEIGRVGETGNAYGYHLHFEYRLASDLETAVDPAPF
                     FTKHGINLH"
     gene            complement(3900..4745)
                     /locus_tag="VH419_10585"
     CDS             complement(3900..4745)
                     /locus_tag="VH419_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020392410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY7044102.1"
                     /translation="MLRPTVRAFSALSAVAVEVGWVTAHAATYPLGLIRDRGDDPDPG
                     RFRFGDLPLAQRGLIGADLEAATTPIVLIHGIVDNRTIFTVLRRGLRKRGFGSLRSFS
                     YGPQTNDLRATAARFGAFVEAVVDEAGAGRVHVIGHSLGGLIARYYVQRLGGDARVAT
                     LVTLGTPHQGTLAARLLPNRLVRQLRPGSDVMLELAEPTDCSTRFLSFYSDVDHLVVP
                     ARHARLEHPDLAARNVLVRGVGHMSLPLSGRIVHEICATLVDRSVGPGTTSNSHQIGQ
                     RRTSA"
     gene            4984..5391
                     /locus_tag="VH419_10590"
     CDS             4984..5391
                     /locus_tag="VH419_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020576913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HEY7044103.1"
                     /translation="MAPIRVVIAKPGLDGHDRGAKVIARALRDAGMEVIYTGLHQSPQ
                     QIVETALQEDADIIGLSVLSGAHMTLFQKVIELLTERSADDILVFGGGIIPEADIPLL
                     EEAGVAKIFTPGTKTQDVVDWVNAALSSEPSDA"
     gene            complement(5534..6433)
                     /locus_tag="VH419_10595"
     CDS             complement(5534..6433)
                     /locus_tag="VH419_10595"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY7044104.1"
                     /translation="MLTRLDVRSAGVSLAVWQGPRNGPTIVFVHGYPDTHVLWDPVIE
                     RLVDRFHCVAYDVRGAGESGAPTDAGGYRLGYLRADVAAVIDAVQDEASVSDRVHLVG
                     HDWGSVQLWDAVIRSGSDARLAGRLASFTSISGPCLEHVGAWMRSARRGTWKHRRAVL
                     RQLLHSWYVLAFQVPVLPEIVLRRVNRRLLRRRPRASHHFAPSLPDDSVRGLGLYRAN
                     ILSRREPLPGGATTDLPVQVIVLLRDPYVTSACLSDLQRWTSDLTRVELDAGHWAPYT
                     HADEVVSLIARHVDSHAPPPPDR"
     gene            6549..7715
                     /gene="sucC"
                     /locus_tag="VH419_10600"
     CDS             6549..7715
                     /gene="sucC"
                     /locus_tag="VH419_10600"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020391514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEY7044105.1"
                     /translation="MDLMEYQAKELFAKHDVPVSMGVMVENAEDARAAAEKLGGRVVV
                     KAQVKTGGRGKAGGVKLAETPDDAVAKANEILGMDIKGHPVRRVLIAPTADIADEYYF
                     SFLVDRAARRYLCIASTAGGVEIEVTAHENPDAVAQIGIDPLVGVDEAKAREIVEATG
                     FPADVADEAVEVIQKLWTVFVEEDASLVEVNPLVRLGNGHLDALDGKVTLDDNADFRH
                     SDHSVFEDKASADPLEALAKEKDLNYVKLDGEVGIIGNGAGLVMSTLDVVAYAGERHN
                     GVKPANFLDIGGGASAQVMADGLHIVLGDSQVKSVFVNVFGGITACDEVANGIVNALR
                     MLGDEANKPLVVRLDGNNVEEGRRILQDANHPLVIMVDTMDGAADRAAELAAAA"
     gene            7726..>8472
                     /gene="sucD"
                     /locus_tag="VH419_10605"
     CDS             7726..>8472
                     /gene="sucD"
                     /locus_tag="VH419_10605"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002514757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEY7044106.1"
                     /translation="MAIFLNENSRVIVQGMTGSEGSKHTQRMLDAGTNIVGGVTPGKG
                     GQSVDFRGTTVPVFGSVAEAVEATGADVSVVFVPPRFTKSAVVESVDAGVALVVVITE
                     GVPVKDTAEFFAYAQSKGTSRIIGPNCPGVISPGKSNAGIIPADITKAGRIGLVSKSG
                     TLTYQMMYELRDFGFSSCVGIGGDPIIGTTHIDALQAFQDDLETDAVVMIGEIGGDAE
                     ERAAAYIQEHITKPVVGYVAGFTAPEGKTMG"
BASE COUNT         1404 a         2769 c         2945 g         1354 t
ORIGIN      
        1 ggcctccggt ggttgagctt gtcgaaaccc ctcgatctcg acgggctcga tcagcgggct
       61 actcgacggg ctcgatcagc gggcctccgg tggttgagct tgtcgaaacc cctcgatctc
      121 gacgggctcg atcagcgggc cttcggcggt tgagcttgtc gaaacccctc gatctcgacg
      181 ggctcgatca gcgggctact cgacgggctc gatcagcggg gacgttcaca acttctcaac
      241 gggggagtag cgcagcagca ggcgtttgac gccgagcgaa ccgaagtcga tcgatgcgac
      301 cgctttctcg gccacgcctt cgacggtgac gaccgaaccc atgccgaacg tgtcgtggac
      361 gacccggtca ccgggctcga gggacgggac cggccggctg cccttggtcg gctgtggtgc
      421 gggtgcagcg gaggcgcgtc gccgctgcac cgtggcggcg gccgggatgt agccactccg
      481 atcggcgtac ctggtcgacg ccgacgagct gaaccacgac gtcaggtccg cctcggtgcg
      541 cttccagtcg atgagcgtct cgggcagctc cgcgaggaac cgtgacgacg ggttgtgctg
      601 cggtgccccc cacgccgacc gcatgacggc acgcgacacg tacagccgct gcttggcacg
      661 cgtcaggccg acgtacgcga gccggcgttc ttcttcaagc tccgctacgt ctcccacggc
      721 acgtgagtgc ggaaagatcc cgtcctcgag tccggtcagg aagacgaccg ggaactcgag
      781 ccccttggcc gtatggaggg tcatcagcgt caccacaccc ccggagtcgt cctcgtcggg
      841 gatctggtcg gagtcggcga cgagcgccac ccgttcgagg aagtcgccca aggttgcttc
      901 gtcgatctcg tcggcgaact cgcgggcgac cgcgacgagc tcgccgaggt tctcgacgcg
      961 cgtctcgtcc tgcgggtcgt cggaggcttc cagctccgcg agatagcccg acctggtgag
     1021 cacagcctcg agcacctcgt cgggtcgggt tccagcctcg accatcgtct ggaagtcctc
     1081 gatcatggcg acgaaggcct ggatgttctt caacgagcgg gtggcgagct ccggcgcgtc
     1141 ctcggccttg cgcagcgcct cccagtacga cgcaccggtg cgttcggcca acatctcgac
     1201 gcatgcctcg gcacgctctc cgataccgcg cttcggcacg ttgaggatcc ggcgcagcga
     1261 caccgtgtca tcgggattga ccagcatccg aagatacgcg agcgcgtccc gcacctcgcg
     1321 acgttcgtag aaccggacac cgcccacgac cttgtacggc aggccgacgc ggatgaagac
     1381 ctcctcgaac acccggcttt gtgcgttggt ccggtagaag acggcgacgt cgctggggtg
     1441 cgccagtcct tcgtcggtca accggtcgat ctcctccacg acgaactgcg cctcgtcgtg
     1501 ctcgttgtcg gcgacgtacc cgacgatctg ctcaccgtcg ccggcgtcgg accacaggtt
     1561 cttcggctta cggctcttgt tgcgtgagat gacggcgttc gccgctgaca agatcgtctg
     1621 cgtcgagcgg tagttctgct cgagcagcac cgtggtggcg ttcgggaaat cctcctcgaa
     1681 cgccaggatg ttgcggatgc tggcaccgcg gaacgcgtag atcgactggt cagcgtcgcc
     1741 gacgaccatc agctcggccg gctcggcagc gtgctcgtcg gggccttcgc cgctcatgcc
     1801 ccccgaacct gcgccgacga ggtcgccgtc gatcgtgcgg cggccaacgc cgtgcccgca
     1861 cagctcgcgg acgaggacgt actgggcatg gttggtgtcc tggtactcgt cgacgaggac
     1921 gtggcggaag cgccgacggt aggtctcccg cacctccggg tagccctgga agagctgcac
     1981 cgtggtcatg atcaggtcgt cgaaatcgag tgcgttggca gcgcgcagcc gagtctggta
     2041 cgtcgtgtag cactctgcga acgtgcgttc gaggtggttg ctcgccttcg cggccgcggc
     2101 gtcggggttg agcagctcgt tcttgcagtt gctcacccag ttgaggatcg agcgtggcgc
     2161 gtagcgcttc aggtccaggt cgaggtcgcg acagacgagc gtcatcagcc ggcgcgcgtc
     2221 ggcctcgtcg tagatcgaga agttggatcc gtagccgagc cggctcacct cgcggcgaag
     2281 gatgcgcaca caggctgagt ggaacgtcga gacccacatg atcttcgccc ggccacccac
     2341 gagctcctcg acgcgctcgc gcatctcggc cgcggccttg ttggtgaacg tgatcgcgag
     2401 cacggatccg gggtgcacgt tgcgctgtga cagcagccag gcgatccgtc gggtcagcac
     2461 tcgcgtcttg ccggatccgg ctcctgcgac gaccaacagc gctgtgccct cgtgcgtcac
     2521 ggcctcgcgt tgcgacgcgt tcagaccctc gaggagatct tcggccgtca ggcgacggcg
     2581 gcgcggccgg ccctcggcgt cggggtcttc gacgggctcg cccgaacggg ggggatcgac
     2641 cagaggcggc aacgggatga gcgcggacat gtccctcaca tgctacgtgg ctggaccgac
     2701 cacactccgg tggtcgagcc ggtcgaaacc cacgacgtgt cggagcccct cgatctcgcc
     2761 aggctcgatc aacgggtctc gacagactcg atccgcgggg tctcgacagg gctgatcagc
     2821 ggggtgcgga tcagtggagg ttgatgccgt gcttggtgaa gaacggtgcc gggtcgacgg
     2881 cggtctcgag gtcggacgcg agccggtact cgaagtgcag gtggtagccg taggcgtttc
     2941 cggtctcccc gacgcggccg atctcctgac ccttgaggac ggtttcgccc ttggtcacct
     3001 cgatcgcgga caggtggttg taccagacct gatcgccgtt ctcgaactgc aggcggacct
     3061 ggttgccgta gggtccgtcc catccggtct gcgccacctt ggcgttcgcc accgcgaccg
     3121 caggagtgcc gtacgccgtc gcgaagtcga tgccggtgtg gtacccggac tcccagtagc
     3181 cgccggcctc cccgaaccag acactgatgt ggaagccact cgtcggcagc acccactggt
     3241 cgctcttgag ctcacgggcg tactgctgtg catcgctggc cgccgagcgg agcagcgcaa
     3301 ccctcgcctt ggcctgtcgt tcggcggcgt tctgcagggc gggtttcgtc acggtgatgt
     3361 tgggccggtc accggcccga ctcacccggg tcgcgtcacg accctcgagc gatgccgacg
     3421 agaggttggc gacatacatg ccgcgctggt gggacgccgt ggaaaccgtg ccactggaag
     3481 acaccgcctg gccggccgtg gctgcaccga tggccgcggc gagcagcgcg ccaccgccga
     3541 ccatcgacgg cagcgagacc ccgagcgatc gacgcggcgc cttcggcgcc cgccgacggc
     3601 cgcctgccgg cacagccctg gccgcacgac gggtgcggcc gacggacccc cgagtggcgc
     3661 ggtgcttctc agcacggtgc gacggcacgc tggcgggtcc ttccgggaca ggggtggctg
     3721 tcgggcctgg tcatccgatc ggaatccgcc caacgtgccg gggggcacaa agatccgagg
     3781 gtaacggaac ggatacgagg aggaaaatcg agggagaccc ggtctgtggt gcccactgag
     3841 gacaaccatc cgtttggcca ctgtgcgtgt ggctcagatg actcatcgtg atgatccgtt
     3901 catgcagacg tacgccgctg cccgatttga tgggagttcg atgtcgtccc cggtccgacc
     3961 gaacggtcga ccagcgtggc gcagatctcg tgcacgatcc gaccgctcaa cggaagcgac
     4021 atgtggccca cgccacggac gagcacgttg cgcgcggcca ggtcgggatg ctcgagccgg
     4081 gcgtgccggg cggggacgac cagatggtcc acgtcgctgt agaaggagag gaagcgggtc
     4141 gagcagtcgg tcggctcggc cagttcgagc atcacgtcgc tgccgggccg gagctgacgg
     4201 acgagccggt tcggcagcag ccgcgccgcc agggtcccct ggtgcggcgt accgagcgtc
     4261 accagggtcg ccacccgggc gtcgccaccg agccgctgca cgtagtaacg cgcgataagg
     4321 ccgcccagcg agtgaccgat cacgtgcaca cggccggctc cggcttcgtc gacgacggcc
     4381 tcgacgaacg cgccgaaccg cgcggccgtc gctcggaggt cgttggtctg cggcccgtac
     4441 gagaaggacc tcagggaacc gaacccgcgc ttgcgaaggc cccgacgcag cacggtgaag
     4501 atggtccggt tgtcgacgat gccgtggatg agcacgatcg gtgtcgttgc tgcttccagg
     4561 tcagctccga tgaggccgcg ctgcgcaagc gggaggtcgc cgaagcggaa gcggccgggg
     4621 tcggggtcgt cgccacggtc ccggatgagc cccagggggt aggtcgcggc gtgtgcggtg
     4681 acccagccca cctcgaccgc gaccgcggaa agggcggaga acgcgcgcac cgtcggcctc
     4741 agcaccgggg gcacatcctg ggatccgtcc ttgttcgtcg acgcgtgaca gtggtcacac
     4801 gggccagcca acgcagggta tcggctccga acgggtcggg cacgccgact gggcgtttcg
     4861 tccccacgaa cccgattggg tcgcgtcgac gtcctgtcgt tagtctgcag gggttgccgg
     4921 cggcggagcc gagtcggcaa cggtgaccgc gccgatggcg cccgacaagg cggtgaacag
     4981 ctgatggccc ctattcgggt ggtgatcgcc aagcccggcc tcgacgggca cgaccgggga
     5041 gcgaaggtca tcgcgcgggc actgcgggat gcaggcatgg aggtcatcta caccgggctg
     5101 caccagtcgc ctcaacagat cgtcgagacc gcgctgcagg aggacgccga catcatcggg
     5161 ctgtcggtcc tgtcgggtgc gcacatgacg ctgttccaga aggtgatcga gctgctgacg
     5221 gaacggtcgg ccgacgacat cctcgtgttc ggtggcggga tcatccccga ggcggacatc
     5281 ccgttgctcg aggaggccgg agtggccaag atcttcaccc ccggcaccaa gacgcaagac
     5341 gttgtcgact gggtcaacgc cgcgctgtcc agcgagccct ccgacgcctg acccctctcg
     5401 ccgaggtggc aagtttcgtt gcagactcgc cggttccata acagcgattg ccacctcgat
     5461 cgctcttcac cgaaagccgc cgtgctgtca cctgggtgcg taccgacacg tacggacctt
     5521 ccaggagacg ctcctagcgg tctggcggtg gcggcgcatg gctgtcgacg tgtcgtgcaa
     5581 tgagtgaaac cacctcgtcg gcgtgcgtgt acggcgccca gtggccggcg tcgagctcca
     5641 cccgcgtcag gtcggatgtc cagcgttgga ggtcgctgag gcacgcgctc gtcacgtacg
     5701 ggtctcgcag cagcacgatc acctggacgg ggaggtcggt cgtcgcacct ccggggagcg
     5761 gctcgcgacg gctgaggatg ttggcgcgat agagcccgag tccacgaacc gagtcgtcag
     5821 ggagggaggg cgcgaagtgg tggctcgccc gcggtcgccg acgcagcagc ctgcggttga
     5881 ctcgacgcag cacgatctcg ggcagcaccg ggacctggaa cgcgaggacg taccacgagt
     5941 gcagcagctg acgcagtacc gcgcgcctgt gcttccaggt gccacgccga gctgaccgca
     6001 tccacgcccc gacgtgctcg aggcacggcc cactgatcga ggtgaacgac gccaaccgtc
     6061 ccgccaggcg tgcgtcggaa cccgaccgga tgaccgcgtc ccagagttgc accgaacccc
     6121 agtcgtggcc gacgaggtgc acgcggtccg agacgctcgc ttcgtcttgc acggcatcga
     6181 tgacggcggc gacatcagca cgaaggtacc cgaggcggta accgccggca tcggtcggcg
     6241 cacccgactc cccggcgccg cggacgtcgt acgcaacgca atggaaccgg tcgacgagac
     6301 gctcgatcac gggatcccag aggacatggg tgtcggggta gccgtggacg aagacgatgg
     6361 tcggaccgtt gcgcgggcct tgccacaccg ccagcgagac acccgcggat cggacgtcga
     6421 gacgggtcag catgaatcgt cacctcctcc cggtgtgggg cgcgcgaccc cgagtctgcc
     6481 aaagcagcgc cgtctggcta ggctccaaac gtctacctcc gccgatcact cgaaggacgg
     6541 cactgaccgt ggatctgatg gagtaccaag cgaaggagct cttcgccaag cacgacgtcc
     6601 ccgtctcgat gggcgtcatg gtcgagaacg ccgaggacgc ccgcgccgcc gccgagaagc
     6661 tcggtgggcg cgtcgtcgtc aaggcccagg tgaagaccgg cggccgcggc aaggcgggcg
     6721 gcgtcaagct cgccgagaca cctgatgacg ccgtcgcaaa ggcgaacgag atcctgggca
     6781 tggacatcaa ggggcacccg gtccgccgcg tgctgatcgc accgaccgcg gacatcgcgg
     6841 acgagtacta cttctcgttc ctcgtcgacc gcgcggcgcg tcgatatctg tgtatcgcga
     6901 gtaccgccgg cggtgtcgag atcgaggtga cggcgcacga gaatcccgat gccgtcgccc
     6961 agatcggcat cgacccgctg gtcggcgtcg acgaggcgaa ggcgcgggag atcgtcgagg
     7021 ccaccggctt cccggccgat gtcgccgacg aggccgtcga ggtcatccag aagctgtgga
     7081 cggtgttcgt cgaggaggac gcgtcgctgg tggaggtcaa cccgctggtt cggctcggca
     7141 acggccatct cgatgcactc gacgggaagg tcaccctcga cgacaacgcc gacttccggc
     7201 actccgacca ctcggtgttc gaggacaagg cgtcggccga cccgctcgag gcgctcgcca
     7261 aggagaagga cctcaactac gtcaagctcg acggcgaggt cgggatcatc ggcaatgggg
     7321 cgggcctcgt catgtcgacg ctcgacgtcg tcgcgtacgc cggcgagcgt cacaacggtg
     7381 tgaagcctgc caacttcctc gacatcggtg gcggcgcctc ggctcaagtg atggccgacg
     7441 gcctgcacat cgtcttgggc gacagccagg tgaagagcgt cttcgtcaac gtgttcggcg
     7501 gtatcaccgc gtgcgacgag gtggccaacg ggatcgtgaa cgcgttgcgg atgctgggcg
     7561 acgaggccaa caaaccactg gtcgtacgac tcgacggaaa caacgtcgag gagggacgac
     7621 gcatcctgca ggacgcgaac cacccgctcg tcatcatggt ggacacgatg gacggtgcgg
     7681 ccgaccgtgc cgccgagctc gctgcggctg cgtgaggaga cgacgatggc gatcttcctg
     7741 aacgagaaca gccgggtcat cgtgcagggc atgaccgggt cggagggctc caagcacacc
     7801 cagcgcatgc tcgacgcggg gacgaacatc gtcggcggcg tcacccccgg caagggtggt
     7861 cagtccgtcg acttccgggg tacgactgtt ccggtgttcg gttcggtggc ggaggccgtc
     7921 gaggcgaccg gtgctgacgt gagcgtcgtc ttcgtaccgc cgcggttcac gaagtcggcc
     7981 gtggtcgagt ccgtcgacgc gggagtcgcg ctggtcgtgg tgatcaccga aggggtgccc
     8041 gtaaaggaca cggcggagtt cttcgcctac gcgcagtcca agggaacgtc acggatcatc
     8101 gggccgaact gtcctggtgt catcagcccg ggcaagtcca acgccggcat catcccggct
     8161 gacatcacga aggccgggcg gatcgggctg gtgtcgaagt ccggcacctt gacgtaccag
     8221 atgatgtacg agctgcgtga cttcggcttc tcgtcgtgcg tcggcatcgg tggcgacccg
     8281 atcatcggca ccacccatat cgacgcgctg caggcgttcc aggacgatct cgagaccgac
     8341 gccgtcgtga tgatcggcga gatcgggggc gatgcggagg agcgcgccgc ggcgtacatc
     8401 caggagcaca tcaccaagcc ggtcgtcggc tacgtcgccg ggttcactgc gccggagggt
     8461 aagacgatgg gt
//
LOCUS       DATBFZ010000344         1701 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_2206471, whole genome shotgun sequence.
ACCESSION   DATBFZ010000344 DATBFZ010000000
VERSION     DATBFZ010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2206471"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1701
                     /locus_tag="VH419_10610"
     CDS             <1..>1701
                     /locus_tag="VH419_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HEY7044107.1"
                     /translation="VAFDVSRQTFRTEEYAEYKANRSTSPSEFSGQVSLIKEVLDALR
                     IPYLEKPGFEADDVIGTLATQAVAAGYDVLICSGDRDSFQLVNDRTTVIYPMRGVSEM
                     KRMTPDAVMERYGVPPERYPELAAIVGETSDNLPGVPGVGQGFAAKWLNQYDGLDGVI
                     ANAEQITGKKGESLREHLGDVLRNRQLNALVCDLELPKRPADLAVQPWDRDEVHKIFD
                     GLEFRVLRDRLFETLSADEPDIAAGTEIDGTVIGSGQLAGWLAEHASGDTATGVEVQG
                     TWGAGTGNVTSVALAESGGVTAWVDATEIGPDDETVLAAWLGDPERPKVLHDAKGPML
                     ALAARGWPLSGVVADTALSAYLSHPDQRSYDLADLTLRYLHRELKAAAGSTDQLSFDL
                     DGDSELAQESMQKANAVVDLGDALETELATRGGSSLLADVELPLVDVLAKMEQVGIGV
                     DVDHLTSLEAQFAGRVKQAADDAYAVIGKQINLGSPKQLQVVLFDELGMPKTKRTKTG
                     YTTDAEALQGLYEKTEHPFLQHMLIHRDVTRLRQTVEGLLKTVADDAYAVIGKQINLG
                     "
BASE COUNT          286 a          595 c          573 g          247 t
ORIGIN      
        1 ccgtggcctt cgacgtgtcg cgacagacgt tcaggaccga ggagtacgcc gagtacaagg
       61 ccaaccgctc gacctccccg tccgagttct ccggccaggt ctcgctcatc aaggaggtcc
      121 tcgacgcgtt gcggatcccc tacctcgaga agccggggtt cgaggcggac gacgtgatcg
      181 gaacgctcgc gacccaggcg gtcgccgccg gctacgacgt gctcatctgc agcggcgacc
      241 gcgactcgtt ccagctcgtc aacgaccgca ccaccgtgat ctacccgatg cggggcgtgt
      301 ccgagatgaa gcggatgacg ccggacgcgg tgatggagcg ctacggcgtc cccccggagc
      361 gctatccgga gctcgccgcg atcgtcggcg agacgtccga caacctcccg ggcgtgccag
      421 gggtcggtca gggcttcgcc gcgaagtggc tcaaccagta cgacgggctg gacggtgtca
      481 tcgcgaacgc cgagcagatc accggcaaga agggcgagtc cctgcgcgag catctcggcg
      541 acgtgctccg caaccgtcag ctcaacgcgt tggtctgcga cctcgagctg cccaaacggc
      601 cggccgacct cgccgtccag ccctgggatc gcgacgaggt gcacaagatc ttcgacggcc
      661 tggagttccg cgtgctgcgt gaccggttgt tcgagacgct gtcggccgac gagcccgaca
      721 tcgccgcggg caccgagatc gacggcaccg tgatcggctc cggccagctg gccgggtggc
      781 tcgccgagca cgcctccggc gacaccgcga ccggagtcga ggtgcagggc acctggggag
      841 ccggcaccgg caacgtcacg tccgtcgcgt tggccgagtc aggaggtgtc actgcctggg
      901 tagacgccac ggagatcggt ccggacgacg agaccgtgct cgcggcgtgg ctcggcgatc
      961 cggagcgtcc caaggtgctg cacgacgcca aggggccgat gctggccctg gcggcccggg
     1021 gttggccgtt gtcgggtgtc gtcgccgaca ccgcgctgtc ggcatacctc tcccaccccg
     1081 accaacgctc ctacgacctg gccgacctga cccttcgcta cctgcaccgc gagctcaagg
     1141 ctgccgcagg ctcgaccgac cagctgtcct tcgacctcga cggggactcc gagctcgcgc
     1201 aggagtcgat gcagaaggcc aacgccgtcg tcgacctcgg tgacgcgctc gagaccgagc
     1261 tcgccacccg tggcggctcg agcctgctcg ccgacgtcga gcttccgctg gtcgacgtgc
     1321 tcgccaagat ggagcaggtc ggcatcggcg tcgacgtcga ccacctgacc tcgctcgagg
     1381 cgcagttcgc cgggcgggtc aagcaggcgg ctgacgacgc gtacgccgtg atcggcaagc
     1441 agatcaacct cggctccccg aagcagctgc aggtggtgct gttcgacgag ctggggatgc
     1501 cgaagacgaa gcgcaccaag acgggttaca ccaccgatgc cgaggccttg caggggctct
     1561 acgagaagac cgagcacccg ttcctgcagc acatgctgat ccaccgcgac gtgacccggc
     1621 ttcgtcagac cgtcgagggc ctgctcaaga ccgtggccga cgacgcgtac gccgtgatcg
     1681 gcaagcagat caacctcggc t
//
LOCUS       DATBFZ010000345         2289 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_6027979, whole genome shotgun sequence.
ACCESSION   DATBFZ010000345 DATBFZ010000000
VERSION     DATBFZ010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6027979"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /locus_tag="VH419_10615"
     CDS             <1..509
                     /locus_tag="VH419_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020389585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY7044108.1"
                     /translation="ILRYVAKMTINPAITLGIADLLGSIAPGKMADLVLWEPGFFAAK
                     PKIVIKNGMILWSTMGDPNASVPTPQPVLYRPMFGAYGGALSANCITFVSRIAYEDGI
                     GERLRLRRRLVPVRGTRGLTKRDMVRNDALPNIEVDPETFAVRVDGVHATVPPARTLP
                     LTQLYFFS"
     gene            561..1880
                     /locus_tag="VH419_10620"
     CDS             561..1880
                     /locus_tag="VH419_10620"
                     /inference="COORDINATES: protein motif:HMM:NF013858.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory UreF family protein"
                     /protein_id="HEY7044109.1"
                     /translation="MVVTAVLGNVKDRDWAGRLERGHVDKLLLDQWEAQKGRLRKRTV
                     AGVEVGLALRRGTRLSDGDILAWDEESATAIVAHIQLGDVMVIALSGLAAQPTSVALR
                     SAIELGHAIGNQHWPAVVKDDQLYIPLTVDRAVMNSVMRTHDLIGISHQFLPGAEVIA
                     FLAPHESRRLFGGANSTIHSHLPEPASREPETQRRVLCAPARPRGSMTPTPASINATT
                     RILQFGDSMFPVGTFAFSGGLEAAVQQGVVKDRTDLAEFVETVAHLAATSDGIALLAA
                     HRGAIDRDLARIRAADDAVYVRKLSEEARTMTVRMGRKLAEAAVRILDEPILDARLRE
                     SANDSVPVTYPVALGALFAALDLPEPDAFATHQYGVTTMVLGAAVRLMRIDHLECQEI
                     LYAVNATTADEYQRAAPASLDDMASFGPTIDVLAASHVHAQVRMFMN"
     gene            1880..>2289
                     /locus_tag="VH419_10625"
     CDS             1880..>2289
                     /locus_tag="VH419_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009083826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY7044110.1"
                     /translation="MEAPMKTISRIGIGGPVGSGKTAIIEAIVPRLVGQGLRVLVVTN
                     DVVTTEDAQHVRRALTGVLVGERIVGVETGACPHTAVREDPSMNLAAVEDMEGRFPDS
                     DLLLIESGGDNLTLTFSPALVDYFVYVIDVAAGD"
BASE COUNT          420 a          721 c          745 g          403 t
ORIGIN      
        1 ggatattgcg gtacgtggca aagatgacga tcaaccccgc gatcactctg ggcattgcgg
       61 atctactcgg ctccatcgca cccggcaaga tggccgacct cgtactttgg gagccaggct
      121 tcttcgccgc aaagccaaag attgtgatca agaacgggat gatcctctgg tcgacgatgg
      181 gggaccccaa cgcgtccgtg ccgacgcccc agccggtctt gtaccggccg atgttcggcg
      241 cctacggagg cgcgctcagc gcgaactgca ttacgtttgt gtcgcgaatt gcttacgaag
      301 atggcatcgg cgagcgcctg cgcctacgta ggcgacttgt tcctgtacgg gggacgcgag
      361 gtctgaccaa gcgggatatg gtccgcaacg atgcccttcc aaacatcgaa gtcgacccgg
      421 agacgttcgc ggtgcgcgtt gacggtgtgc atgcgaccgt gccaccggca cgcaccctcc
      481 ctttgactca gctctacttc ttcagctagg cgggcgggga gatgttgaga gactcgaaat
      541 cggcaactaa cacggcagat gtggttgtta ccgcggtact gggcaacgtt aaggaccgcg
      601 attgggctgg aaggctcgag cgcggccacg ttgacaagct gctgctcgac cagtgggagg
      661 cgcagaaggg ccggctgcgc aagcgaaccg tcgccggagt cgaagtgggc ctggcgttgc
      721 gtcggggcac caggctctct gacggtgaca tcctggcctg ggacgaggag tcggcgacgg
      781 ccatcgtcgc ccacatccag ctcggcgacg tcatggtcat agccctgtcg gggctcgccg
      841 cccagccgac ctcagtggct ctccgttcgg ccatcgaact tgggcacgct attggcaacc
      901 aacactggcc ggctgtggtg aaggacgatc agctatacat cccactcacg gtggaccgtg
      961 ccgtgatgaa ctcagtgatg cggacacacg atctcatcgg gatctcgcat cagtttctgc
     1021 cgggcgccga agtcatcgcg ttcctcgcgc cgcacgaatc tcgtcgcctg ttcggcggtg
     1081 cgaatagcac cattcactca catctgcctg agcccgcgtc acgtgagccc gagacacaaa
     1141 gacgagtact gtgcgcgccg gcccgacctc gtggatcaat gacgccgaca ccagcgagca
     1201 tcaacgcgac gacgaggatc ctgcagttcg gcgactcgat gttcccggtt ggcacgtttg
     1261 ccttctccgg cgggctcgag gcggctgtgc agcagggcgt cgtgaaggac cgcaccgatc
     1321 tcgccgagtt cgtcgagaca gtggcccacc tagcggcaac cagcgacggc atcgccctgc
     1381 tcgccgccca ccgcggcgcc atcgaccggg accttgcgcg aatccgagct gcggacgacg
     1441 cggtctatgt gcgcaagttg tcagaagagg ctcggacaat gacggtacgg atggggcgaa
     1501 aacttgccga ggccgcggta cgtatcctcg acgagcccat tctcgacgcg cggcttcggg
     1561 agtcggccaa cgactcggtg ccggtgacct atccggtcgc gctcggtgcg ctgttcgccg
     1621 ctttggatct tccggagccg gatgccttcg cgactcacca gtacggcgtc acgaccatgg
     1681 tgttgggcgc ggccgtacgg ctgatgcgca tcgaccacct cgagtgccag gagatcctct
     1741 acgccgtgaa cgccaccacg gcggacgagt accaacgcgc ggcgccggcc agccttgacg
     1801 acatggcaag ttttggtcca accatcgacg tgctggccgc gtcgcacgta catgcccagg
     1861 tacgcatgtt catgaactga tggaggcccc catgaagaca atcagccgga tcggcattgg
     1921 tggcccggtt ggcagcggca agaccgcgat catcgaggcg atcgttcccc ggctggtcgg
     1981 ccaaggcctg cgggtgctgg tggtcaccaa cgacgtggtc acgacggagg acgcgcagca
     2041 cgtacgacgg gcgctgaccg gggtgctggt aggggagcgc atcgtcgggg tggagacggg
     2101 cgcctgcccg cacacggctg tccgcgagga tccgagcatg aaccttgccg ccgtggagga
     2161 catggaaggg cgctttccgg acagcgactt gctcctcatc gagagcggcg gggacaacct
     2221 aaccctcacc ttcagcccgg cattggtcga ctacttcgtc tacgtcatcg atgtggcggc
     2281 cggcgacaa
//
LOCUS       DATBFZ010000346         4052 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_2208680, whole genome shotgun sequence.
ACCESSION   DATBFZ010000346 DATBFZ010000000
VERSION     DATBFZ010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 4052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4052
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2208680"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="VH419_10630"
     CDS             complement(<1..253)
                     /locus_tag="VH419_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012922648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEY7044111.1"
                     /translation="MTARMDLSSVAPAAYRAMIALDTRSHQGPLAAVLLDLVKLRASL
                     INGCAYCVDSHSSDLRSAGEPEPRIAGVAAWREGPFFTAD"
     gene            complement(458..1411)
                     /locus_tag="VH419_10635"
     CDS             complement(458..1411)
                     /locus_tag="VH419_10635"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044112.1"
                     /translation="MEPPSADELGELDSFGTSGMWSVFGRELKVTNLDKVLFPARQGE
                     KPVTKRELLRYSALIAPTLLPYLVRRPLNLHRYPNGAHGKGFWHKAVPSHAPEWLPRW
                     DNPEADEDETPTYLVVDEPAALVWAANFGALEWHAWTSRADRPRQPTYAMIDIDPGDT
                     TSWDDVLVLARLHRAALDHLGVFAVPKVTGRRGIQIWVPIAPGPDFDTTRAWVEKLSW
                     SVGQVVPELVSWKWQVSEREGLARLDYTQNAINKTLVAPYSPRPSAGAPVSAPIEWDE
                     LDDPGLTPDGFTIRTILDRIAAKGDLFAALLHHPQHLPRLT"
     gene            complement(1455..2084)
                     /locus_tag="VH419_10640"
     CDS             complement(1455..2084)
                     /locus_tag="VH419_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009080794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase ligase N-terminal
                     domain-containing protein"
                     /protein_id="HEY7044113.1"
                     /translation="MPSSRARAKSKDQLETYRRMRDFARTPEPAGAPTASPRAGSQRF
                     VVQRHRARRLHYDFRLEVDGVLVSWAVPKGPTLDPDVRRAAFHVEDHPIEYIDFEGVI
                     PSGEYGGGDVIVWDAGTWESPADKKTSAARSVARGEIHVDLFGEKLQGRFVLVRTGTE
                     DSGKEDWLLLHKHDEYAEKGWDPENHPRSVLSGRTNDEGQGGPGPVVAL"
     gene            complement(2128..2763)
                     /locus_tag="VH419_10645"
     CDS             complement(2128..2763)
                     /locus_tag="VH419_10645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044114.1"
                     /translation="MDGVIVAAELARGVRIARAAPWWPFLGTNAIATSLLAVGVLAAR
                     DSLGLLSAVLGVGACGGAAAYLLDDEAETVADATPSSRPRRFGWRLPLLAAPAVVGIA
                     GLTALDQRDPSTHWLRLLPMAAGSVAIGVALAAVMRRRGMSTPGDLAGVLTTAIMPLL
                     VVVNPLWRRWVTLTPLGDQPHLARTCAAWTAIVVLCAGVTFACMRDPGRAR"
     gene            complement(2751..3680)
                     /locus_tag="VH419_10650"
     CDS             complement(2751..3680)
                     /locus_tag="VH419_10650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044115.1"
                     /translation="MTIMEIPAAAAPVRTPSTVWPLARIEAKRYARHPLFLTGLFFAL
                     LFSAGEHGPIELDYHVIPSFFIGVFGIIVGYRLTTSTRKSRPVIDASPVSRTMQTAAL
                     CMACVVPAGAGLLVVLMHRMFVLADPFPERFYGTYGTVDRFLIAIVVPAIACAGGPLL
                     GVAVGRWLRFPGAALLTVVVVLVWGNISGYLPTEKWQSDSFGARLLHMAGPYTAFASS
                     NADANLDPTVVHSFTGSPFWYAVWTLALCGLAAAAAMWRGAEGLARRRVGRAFVVIGT
                     VALVALALSVVTGNQHLYDTTEHGTVVSNGWTV"
     gene            complement(3677..>4052)
                     /locus_tag="VH419_10655"
     CDS             complement(3677..>4052)
                     /locus_tag="VH419_10655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044116.1"
                     /translation="TTGLDPEQRVSLRGVLAEHGREATVVIATHQTEDVAALCERVVV
                     LDGGSVRFDGAVQDLVSLADGRVWLADEPDSRARTSWRTGTGRYRNLGDAAPLCAELV
                     EPSLEDAYLLLRASEPGTEVAA"
BASE COUNT          666 a         1426 c         1373 g          587 t
ORIGIN      
        1 cgtcggcggt gaagaacggg ccctcgcgcc aggcggcgac gccggcgatc cgcggctccg
       61 gctcgccggc cgagcggagg tcggacgagt gcgagtcgac acagtacgcg catccgttga
      121 tcaggctggc ccgcagcttg accaggtcga gcagcaccgc ggccagcggg ccttggtggg
      181 agcgggtgtc gagcgcgatc atcgccctgt acgccgcggg tgcgacgctc gacaggtcca
      241 ttcgcgcggt catggtgtct ccttcggtta ccggctgatg tctcgaccgt agaacgagaa
      301 ttggtctgtc tccagatcca atactgatgc gtgtatgcag gccaatttcg cgcgtcggcg
      361 cgacggagat ctgatcgagg tggcaagcgc gttccgtgcg acggcgtgtc gccaacgaaa
      421 gttgccacct cggcgggagg ggggagtagg gacagcgtca ggtgagacgg gggaggtgtt
      481 gagggtggtg gaggagggcg gcgaagagat cacccttggc ggcgattcga tcgaggatgg
      541 tgcggatcgt gaacccgtcc ggcgtcagac ccgggtcgtc gagctcgtcc cactcgatcg
      601 gggcggagac cggcgcgccg gcgctcggcc tcgggctgta cggcgccacc agcgtcttgt
      661 tgatcgcgtt ctgggtgtag tcgagtcggg ccaacccttc ccgctccgac acctgccact
      721 tccagctcac cagctccgga acgacctggc cgacactcca ggacagcttc tccacccatg
      781 ccctggtcgt gtcgaagtcc ggcccgggcg cgatcgggac ccagatctgg atcccccgtc
      841 gtccggtgac cttcggcacc gcgaacacac cgaggtggtc cagcgcggcg cgatggagac
      901 gtgcgaggac gagcacgtcg tcccaggagg tcgtgtcgcc cggatcgatg tcgatcatcg
      961 cgtacgtcgg ctggcgcggc cggtcggccc gtgacgtcca ggcatgccac tccagcgcgc
     1021 cgaagttggc cgcccacacc aaagcggccg gctcgtcgac aaccaggtag gtcggcgtct
     1081 cgtcctcgtc cgcctccggg ttgtcccacc ggggcagcca ctcaggggcg tgcgagggaa
     1141 ccgccttgtg ccagaagccc ttgccatgcg ccccgttcgg atagcgatgc aggttgagcg
     1201 gacgacgcac caggtacggc agcagggtcg gcgcgatcag cgccgagtag cgcaacagct
     1261 cccgcttggt caccggcttc tccccctgac gtgccgggaa cagcaccttg tcgaggttgg
     1321 tgaccttgag ctctcgaccg aagacgctcc acatgccgga cgttccgaac gagtccagtt
     1381 cgccgagctc gtccgcgctg ggtggttcca ccggtcctgc cttcaaggcg atggacgctc
     1441 gcgcagccgg aaggtcagag cgccacaacc ggtccgggtc cgccttgacc ctcgtcgttg
     1501 gtccggccgc tcaggaccga gcgcggatgg ttctccgggt cccacccctt ctcggcgtac
     1561 tcgtcgtgct tgtgcagcag cagccagtcc tccttgccgg agtcctccgt gccggtcctg
     1621 acgagcacga agcgtccttg cagcttctcg ccgaacaggt cgacgtggat ctcgccgcgc
     1681 gccaccgacc gcgctgcgct ggtcttcttg tctgccggtg actcccaggt gccggcgtcc
     1741 cagacgatga cgtcgccgcc gccgtactcc ccggacggga tcacaccttc gaagtcgatg
     1801 tactcgatgg ggtggtcctc gacgtggaac gccgcccggc gtacgtccgg atcgagcgtc
     1861 ggtcctttcg gcaccgccca gctcacgagg acgccgtcga cctccagccg gaagtcgtag
     1921 tgcagccgtc gagcccggtg ccgctgcacg acgaaccgct gcgagcctgc tcgaggagac
     1981 gccgttggag cgcctgccgg ttcgggcgtc ctcgcgaagt cccgcatccg gcgatacgtc
     2041 tccaactggt ccttggactt cgcgcgagca cgagacgacg gcacgcgtcc agtcaagtgc
     2101 ccggcatcac cagtggcaac cgcgcggtca acgtgcgcga ccggggtctc gcatgcatgc
     2161 gaacgtcact cctgcgcaca gcaccacgat cgccgtccaa gcggcgcagg tccgcgccag
     2221 gtgcggctgg tcgccgagcg gcgtgagcgt cacccaacgc cgccacagcg ggttgacgac
     2281 caccagcaag ggcatgatcg cagtcgtcag gactccggcc aggtcgcccg gcgtcgacat
     2341 gccgcgccgt cgcatcaccg cggccagtgc gacaccgatg gcaacagatc cagccgccat
     2401 cgggaggagc cgcagccagt gcgtcgacgg gtcgcgctgg tccagtgccg tcaaccccgc
     2461 gatccccacc acggccggcg cggccaacag cggcaaccgc cagccgaacc gccgcggtcg
     2521 cgagctgggc gtcgcgtcgg ccaccgtctc cgcctcgtcg tcgagcaggt acgccgcagc
     2581 accgccacac gcgccaacgc cgagcacggc gctgagcagc cccagcgagt cgcgggcggc
     2641 gagcacaccg accgccaaca ggctggtggc gatcgcgttg gtgcccagga acggccacca
     2701 tggagcggcc cgggcgatcc gcaccccccg agccagctcc gcggcgacga tcacaccgtc
     2761 cacccgttcg acacgacggt tccgtgctcg gttgtgtcgt agaggtgctg gttgccggtg
     2821 acgacggaca gcgccagcgc gacgagtgcg actgttccga tgacgacgaa cgcccgccca
     2881 acccgccgcc gggccaggcc ctcggcgccc cgccacatcg ctgccgcagc agcaagcccg
     2941 cacagcgcca gcgtccacac ggcgtaccag aacggcgaac cagtgaacga gtgcaccacg
     3001 gtcgggtcca ggttcgcgtc ggcgttgctt gacgcgaacg ccgtgtacgg acctgccatg
     3061 tggagcaacc gggctccgaa ggagtcgctc tgccacttct ccgtcggcag gtagcccgag
     3121 atgtttcccc agacgagaac gacgacgacg gtcagcaggg cagcgcccgg gaagcgcagc
     3181 cagcgaccaa cggccacgcc gagcagcggt cctccggcac acgcgatggc tggcaccacg
     3241 atcgcgatga ggaaacggtc gaccgtgccg taggtgccgt agaagcgctc cgggaacggg
     3301 tcggccagca cgaacatccg gtgcatgagg acgaccagga gcccggctcc tgcgggtacg
     3361 acgcaggcca tgcagagagc ggcggtctgc atcgtgcgag agaccggtga ggcgtcgatg
     3421 accggccgcg atttgcgcgt cgacgtggtg agccggtagc cgacgatgat gccgaagacc
     3481 ccgatgaaga acgacgggat cacgtggtag tcgagctcga tcggtccgtg ctcgcccgcg
     3541 ctgaacaaca gcgcgaagaa caacccggtg aggaacagcg gatgacgcgc gtaccgtttc
     3601 gcctcgatcc tcgcgagcgg ccagacggtc gaaggcgttc gcaccggggc ggcggctgcc
     3661 ggaatctcca tgatggtcat gccgccacct ccgtccccgg ctccgacgcg cgcaacaaca
     3721 ggtaggcgtc ctcgagtgac ggctcgacga gctcggcaca aagtggcgcc gcatcgccga
     3781 gattgcggta gcgaccggtc ccggtgcgcc acgacgtacg agcccggctg tccggctcgt
     3841 cggcgagcca gacccgaccg tcggccagcg agacgaggtc ctggactgcc ccgtcgaacc
     3901 ggacactgcc accgtcgagc acgaccaccc gctcgcagag ggcggcgacg tcctcggtct
     3961 gatgcgtcgc gatcaccacc gtggcctcgc gcccgtgctc ggccagcacc ccccgcagcg
     4021 acacccgctg ctcggggtcc aggcccgtgg tc
//
LOCUS       DATBFZ010000347         2451 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_3727255, whole genome shotgun sequence.
ACCESSION   DATBFZ010000347 DATBFZ010000000
VERSION     DATBFZ010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3727255"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(286..1023)
                     /locus_tag="VH419_10660"
     CDS             complement(286..1023)
                     /locus_tag="VH419_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020579717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEY7044117.1"
                     /translation="MDEIEVVVAHHERATLRVGDVFLKIDADQTRTDVEIEAMTIAPI
                     PTPEVLWRKPPVLALAALPGKALGRLGEPSTASPAAWAAAGAAVRMLHDAPLPPWSGR
                     TIDAIASELDNECRWLLTNDVLPSELVTRNRRIAEAALRPWTPAFMHGDLQVAHVFVD
                     GDEITGVVDWSEAGQGDALYDLASLTLGHRDHLGDVVTGYGTDVDVDVIRAWWSLRSL
                     TAVRWLVEHGFDPSASGCEVDVLRSAL"
     gene            1162..1521
                     /locus_tag="VH419_10665"
     CDS             1162..1521
                     /locus_tag="VH419_10665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044118.1"
                     /translation="MSDEPVPIAYTALQPGTPVQTSDGHEFGSVESVLAVEEVAVFDG
                     IVVACEKGTRFVDADYVGAIFTTHVVTTLSAEEAAHLPLPDQDPVYDTDASDDTGASL
                     SDRLGRMFGRGKWKRER"
     gene            1658..2245
                     /locus_tag="VH419_10670"
     CDS             1658..2245
                     /locus_tag="VH419_10670"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEY7044119.1"
                     /translation="MAYQAHPDTVEKNSGQPARKVVAGYFLSLDGVAEDPDRFITAWD
                     DETDASGADLIATQDTVILGRRSYDEWAEFWPDSEIEPFASFINAVPKYVATSTPLER
                     EWSNSRVIEGGLVDFVRRLKDRQGGDIGVHASISVTRTLLAADVVDELRLVIAPTIVG
                     SGQRLFEGLPAIRLETISNTASPSGHLLVAYRVAH"
BASE COUNT          398 a          868 c          809 g          376 t
ORIGIN      
        1 tccactcaat cgaagtcgcg agtcgatcgc gggtgtctcc tcgcgttcca ccagcacacg
       61 ccgcatccgc accggtgctc gctgccgaga tggccggctg gcagcccaca cgcgtgcgcg
      121 tgatgcgcac atgcgcagtc gccgggcagt ggatggcaca acggactctc ccccttgacg
      181 accccccacg gggtggtctc accatgtcca tacggctccg ccgtcgccga agttcacctc
      241 catctgcgga ctgtggacct gagcagggcg gtacgccggc cggcgtcaca gcgcggatct
      301 cagcacgtcc acctcgcagc ctgacgcgga agggtcgaag ccgtgctcga ccagccagcg
      361 gaccgccgtc aggcttcgca gtgaccacca cgcgcggatc acgtccacgt cgacgtcggt
      421 gccgtagccc gtgacgacgt cgccgaggtg gtcccggtgt ccgagcgtca ggctcgcgag
      481 gtcgtacagc gcatcgccct ggcccgcctc cgaccagtcg accacccccg tgatctcgtc
      541 accgtcgacg aacacgtggg cgacctggag gtcgccgtgc atgaacgccg gtgtccacgg
      601 tcggagcgca gcctcggcga tccggcggtt acgggtcacc aattcgctgg gaaggacgtc
      661 gttcgtgagg agccaacggc actcgttgtc gagctcggag gcgatcgcgt cgatcgtccg
      721 accggaccat gggggcagcg gcgcgtcgtg caacatccgt acggcggcac cggccgctgc
      781 ccacgccgcc ggcgacgcgg tcgacggctc gcccaggcgg ccgagtgcct tcccggggag
      841 ggcggcgagc gcgagcacgg gcggcttccg ccacaggacc tccggcgtcg ggatcggcgc
      901 gatggtcatc gcctcgatct cgacgtccgt gcgtgtctgg tcggcgtcga tcttcaagaa
      961 gacgtcgccg acgcgtaggg tcgcgcgctc gtgatgggcg acgacgacct cgatctcgtc
     1021 cacgccggcc attgtcgcgg gagagccatc gacgtcccca cggcttttgg gccgcgatcg
     1081 gtgccatcgc agacggtttc gagtcctcgg cagagcacgt gcgtcgtggt gtgacccgca
     1141 cccgcgacat aggctcacgc catgagtgac gagcccgtgc ccatcgcgta cacagcgctt
     1201 cagccaggaa caccggtcca gacgagcgat gggcacgagt tcggctccgt cgagtctgtc
     1261 ctcgctgtcg aggaagtggc cgtcttcgat ggcatcgtgg tcgcgtgcga gaaggggacc
     1321 cggttcgtcg atgctgacta cgtcggagcc atcttcacca cacatgtcgt cacgaccttg
     1381 tccgccgagg aggcagcgca ccttcccctt cccgatcagg atcctgtgta tgacaccgac
     1441 gcatccgacg acacgggcgc gtccttgtcc gaccgcctcg gtcgtatgtt cggccgcgga
     1501 aagtggaagc gggagcgcta gccaacccga cgtcctcggc acgccgccca gtacggacgc
     1561 ccgtctgcga tcaggcgaga cccttgtcga aatgccggcg ccgcacccga cgtaggggtg
     1621 cagcgattcg tccgacatcc tccgaaggag tcccaccatg gcgtaccagg cccatcccga
     1681 caccgtcgag aagaactccg gccagcccgc gcgcaaggtc gtcgccggct acttcctctc
     1741 gctcgacggg gtggctgagg atcccgaccg cttcatcacc gcctgggacg acgaaaccga
     1801 cgccagcggc gccgacctga ttgcgaccca ggacacggtc atcctcggcc gccgcagcta
     1861 cgacgagtgg gctgagttct ggccagacag cgagatcgag ccgttcgcct ccttcatcaa
     1921 cgccgtcccg aagtacgtcg cgacgtcgac gccgctcgaa cgcgagtgga gcaactcgcg
     1981 ggtgatcgag ggcgggctgg tcgacttcgt gcggcgcctg aaggatcggc agggcggcga
     2041 catcggcgtc cacgccagca tctcggtgac gcggacgctg ctcgctgccg acgtcgtcga
     2101 cgagctcagg ctcgtcatcg cgccgacgat cgtcgggagc gggcaacgac tgttcgaagg
     2161 gttgccggcg atccggctcg agacgatcag caacaccgcc tcacccagtg gtcacctgct
     2221 ggtcgcctac cgcgtggccc actagcggct cggccggctc gaccaccaga cgtctcggtg
     2281 gtcgagcccg ggacgtctcg gtggtcgagc ccggtgagcg ccagcgagcc ggtgtcgaga
     2341 ccaacatgcc aggacagccc cggggggtct cgacacgtcc tcgcctaacg gctcggccgg
     2401 ctcgaccacc gaagggcaga ctcggccggc tcgaccaccg aaggcagact c
//
LOCUS       DATBFZ010000348         2807 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_237085, whole genome shotgun sequence.
ACCESSION   DATBFZ010000348 DATBFZ010000000
VERSION     DATBFZ010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237085"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2036)
                     /locus_tag="VH419_10675"
     CDS             complement(<1..2036)
                     /locus_tag="VH419_10675"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012921067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEY7044120.1"
                     /translation="MARRTTSAPPEEFDEHIVDVDVRDEMRTSYLEYAYSVIYSRALP
                     DARDGLKPVQRRILYTMHDMGVRPDRGHVKSARVVGEVMGRLHPHGDGAIYDALVRMV
                     QPWALRLPLVDGHGNFGSPDDPPAAMRYSECRMAPAAIAMTQGIDEDTVEFKPNYDSR
                     EFEPIVLPAAFPNLLVNGASGIAVGMATNLAPHNLVEVVQALRHLITHPKATVDDLMR
                     FVPGPDLPTGGKIVGLNGIREAYETGRGSFRMRASARVENVTPRRKGIVVTELPYNVG
                     PERVIERIKTLVQTKKLQGIADVKDLTDRTKGLQLVIEIKNGFHPEAILEQLYRQTPM
                     EDSFGINAVALVDGQPRTLGLKQMLEVYLDHRYDVVRRRSLFRRTKAAERLHLVEGLL
                     IAILDIDEVIQLIRSSEDAATAKTRLIEVFDLSEVQATYILDMPLRRLTKYSTLELEG
                     EKSELQRSIEELTAILEDESLLRKVVGDELSEVAKTYGTPRRTVLLESAGTPVTSAVP
                     LEVADDPCFVLMSSSGLLARSSNDEPLGAAGARAKHDAVMAVAATTARGTIGVVTSRG
                     RLLKLDVLDLPALPPTANAPHLQGGAPITEFLALDPGERAVTLTSFSEDSLGLALGTA
                     AGIVKRVQPEVLSNRDSWDVIRLDDGDVVVGGVELASADDELVFVTSDAQLLHFP"
     gene            2295..2675
                     /locus_tag="VH419_10680"
     CDS             2295..2675
                     /locus_tag="VH419_10680"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY7044121.1"
                     /translation="MSGSAMTGVALLVAGAALGVMVLGLVLVRTWRQLRFAAEHDDLT
                     GVLSRHAILRYTTQALSKRKHDAGRLAVLSVDLDGFHRINEQFSHPVGDEVLRVVAET
                     LATTAEPGDLVGRIGGDEFLVVRP"
BASE COUNT          413 a          905 c         1019 g          470 t
ORIGIN      
        1 ggaaagtgca gcagctgagc atcgctcgtg acgaagacca gctcatcgtc ggcgctggcc
       61 agctcgacac cgccgaccac gacgtcgccg tcgtcgagcc ggatcacgtc ccacgagtca
      121 cggttgctca ggacctcggg ctggacccgc ttgacgatcc cggcggcggt gccgagcgcc
      181 aggccgagcg agtcctcgga gaacgacgtg agggtgactg ctctctcgcc gggatcgagc
      241 gcgaggaact ccgtgatcgg cgctccgccc tgcagatgcg gggcgttcgc ggtcggcggc
      301 agcgcgggca ggtcgagaac gtcgagcttg agcagtcgac cgcgtgaggt gacgacgccg
      361 atcgtgccgc gggccgtggt cgccgccacc gccatcacgg cgtcgtgctt ggctcgtgca
      421 ccggcggcgc cgagcggctc gtcgttgctg ctgcgcgcga gcaggccgct ggacgacatc
      481 agcacgaagc agggatcgtc ggccacctcg agcgggactg ccgaggtcac cggcgttcct
      541 gcggactcga gcagcaccgt tcgccggggc gtgccgtagg tcttcgcgac ctcggacagc
      601 tcgtcgccga cgaccttccg cagcagggac tcgtcctcca ggatcgccgt cagctcctcg
      661 atcgagcgct ggagctcgga cttctcgccc tcgagctcca acgtcgagta cttggtgagc
      721 cgacggaggg gcatgtcgag gatgtaggtc gcctggacct ccgacaggtc gaagacctcg
      781 atgaggcggg tcttggcggt ggcggcgtcc tcgctggaac ggatcagctg gatgacctcg
      841 tcgatgtcga ggatcgcgat cagcaggccc tcgacgaggt gcagccgctc tgctgccttg
      901 gtccggcgga agaggctgcg ccggcggacg acgtcgtagc ggtggtcgag gtagacctcg
      961 agcatctgct tgagcccgag cgtccgtggc tgaccgtcga ccagggccac ggcattgatg
     1021 ccgaacgagt cctccatcgg ggtctgccgg tagagctgct cgaggatcgc ctccgggtgg
     1081 aagccgttct tgatctcgat gaccagctgc aggcctttgg tgcggtccgt caggtccttg
     1141 acgtcggcaa tgccttgcag cttcttcgtc tggaccaagg tcttgatgcg ttcgatcacc
     1201 cgctcggggc cgacgttgta cggcagctcg gtgacgacga tgcccttgcg ccgcggggtg
     1261 acgttctcga cccgcgcgct ggcccgcatc cggaacgacc cccggccggt ctcgtacgcc
     1321 tctcggatcc cgttcagccc gacgatcttg ccgccggtcg gcaggtcagg accgggtacg
     1381 aagcgcatca ggtcgtcgac cgtggccttg ggatgcgtga tgaggtggcg cagcgcctgg
     1441 accacctcga ccaggttgtg cggagccagg tttgtcgcca tcccgaccgc gatgccggag
     1501 gcgccgttga cgagcaggtt ggggaaggcg gccggcagca cgatcggctc gaactcgcgg
     1561 ctgtcgtagt tgggtttgaa ctcgacggtg tcctcatcga tgccctgggt catcgcgatc
     1621 gcggcggggg ccatccggca ctcggagtaa cgcatcgcgg ccggcgggtc gtcgggcgac
     1681 ccgaagttgc cgtgcccgtc gacgagcggc agccgcagtg cccacggctg gaccatccgg
     1741 accagcgcgt cgtagatggc gccgtcgccg tgcgggtgca gtcggcccat cacctcaccg
     1801 acgacgcggg cgctcttcac gtggccacgg tcggggcgta cccccatgtc gtgcatcgtg
     1861 tagaggatcc ggcgctgcac cggcttcagg ccgtcgcgag cgtcgggcag cgcgcgcgag
     1921 tagatgacgg agtacgcgta ctcgagatag ctggtgcgca tctcgtcccg gacgtcgaca
     1981 tcgacgatgt gctcgtcgaa ctcctcgggc ggggcgctgg tggtacgtcg ggccatgggt
     2041 gggcggtctc tcctgcatgc gggtgggcgg tgcgcgctca ttgtctcctg tgacgccacg
     2101 cgcgcacggc agcgacccac agcaggctcg ttggccagcg cagcgtaacc gggagatcac
     2161 ccagcgcagg acttcgccgg gtcaggaacg aaaaagggcg cgccacaccg gcgttgtgcc
     2221 gttccgacgt cgtggcgctg ctacgttccg acgcgacggt gcaggagtga ccggtgtgac
     2281 agaaggggct catgatgtcc gggtctgcga tgacgggagt tgccctgctg gtggcgggcg
     2341 ctgctctggg ggtcatggtg ctggggctgg tgctggtccg gacgtggcga cagctgaggt
     2401 tcgccgccga gcacgacgac ctcaccggtg tgctgtcacg gcacgcgatc cttcggtaca
     2461 cgacgcaggc attgtcgaag cggaagcacg acgccggccg gctggcggtg ctcagcgtcg
     2521 acctcgacgg gttccatcgg atcaacgagc agttctcgca cccggtcggc gacgaggtgc
     2581 tgcgggtggt cgccgagacg ctggccacga cggccgaacc tggcgacctg gtggggcgga
     2641 tcggcggcga cgagttcctc gtggtccggc cctaggtgcg cagacacacc cacgagtggg
     2701 ccgacgggat ccgccaagca ctgtgcgacc ggatggacat cggcgggaca ccgttgcgca
     2761 tcaccgcctc gatcggcctg gtcgtcgccg acgcgtctgt cggtgac
//
LOCUS       DATBFZ010000349         3841 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_3519416, whole genome shotgun sequence.
ACCESSION   DATBFZ010000349 DATBFZ010000000
VERSION     DATBFZ010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 3841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3841
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3519416"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1221)
                     /locus_tag="VH419_10685"
     CDS             complement(<1..1221)
                     /locus_tag="VH419_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020392240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEY7044122.1"
                     /translation="MSTPVRRSMAKGDSMLSRVLYLSGRFAARRPWVVIGTWLVAFLF
                     VLGASNAFGQQLEDSFGAPGLDSQEATELLSAAGSDQAGLTAQVVARPRDDGVFFGAG
                     SAARSALEDVRQLALELPHALGVSEPAISPDGRLAVMRVQYSVLEDLSPNDLENLKAF
                     AAEAEAAYPLQLELGGDLFFAFEEPPTGLGELIGVLAAVLILLVAFGSFIAMGLPIGI
                     AVFGLGLGIASMSLLAYLVDVPSWVAPIGSMVGLGVGIDYALFLLTRHRENLAAGMNV
                     PESAGRAAATSGRAVLFAGGTVVIAILGLAAAGIPFVTAAGAGISLIVLIMVLASLTL
                     LPAFLGLAGQRILGRHRTRAVRAGTPSAGWWHRWGAHVSKHAVAYAVVATSALLALAA
                     PVLALRVGTPDDGSL"
     gene            1353..2507
                     /locus_tag="VH419_10690"
     CDS             1353..2507
                     /locus_tag="VH419_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007077195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEY7044123.1"
                     /translation="MTAKLVRRLYDEPRAPDPPRRVWRDWALVGVIVPTAILEGIFSD
                     HVGWRPVSVAMCVAFAFTLLWRRTYPLAVVVITFGSVIVLDLAWLIGGGGGEPVGLDT
                     TVFIVLLPYSLARWGSGHDVAIGLLFMLAADAMAQVTDYTGVADLVFGTLFLLFPAVL
                     GALVRFAATAHQRELDHVRFREREQLARELHDTVAHHVSAIAVRAQAGRVLAASNPGA
                     AADALDVIEEEASRTLEQMRAMVRGLRDSSAPSVTPQPGVADLEPLARSVGESPRVEL
                     DLTGDLDSLSPAVGVAIYRIAQESITNAVRHARHATRIDIEVVGDTDCVRLTVRDDGS
                     PVSASPSPAGYGLVGMAERAALLGGSLEAGPTSDAGWTVRAMFPKVGSDQ"
     gene            2504..3169
                     /locus_tag="VH419_10695"
     CDS             2504..3169
                     /locus_tag="VH419_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012921871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY7044124.1"
                     /translation="MTIRVLVADDQDLVRTGLAMILRSQADVDVVAEAADGRTAVALA
                     RQLRPDVCLLDIRMPDVDGIEATRQLAGPDVEDPLAVVVITTFDLDEYVHGALRAGAR
                     GFLLKDAGPELLMQAIRAAANGDALIAPSVTTRLLTAFADAHPEQPPAQPVEPLTSRE
                     EEVVRTVACGRTNTEIADELHISLSTVKTHLASVMAKLGARNRVEIAMWAYETHRIPR
                     IPR"
     gene            complement(3239..3694)
                     /locus_tag="VH419_10700"
     CDS             complement(3239..3694)
                     /locus_tag="VH419_10700"
                     /inference="COORDINATES: protein motif:HMM:NF015799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing protein"
                     /protein_id="HEY7044125.1"
                     /translation="MDDDRRERDADVQRQIDALSERVTANRTDIDALATRAEAAEDRA
                     DENEARDDANQLRIDQLEIRADVDREMIAELQADGVLSREHAAQMQEALRSSRQIGAA
                     MGIVMASRQVSEAAAFAILSKASQDSNRKVRDVAHDVVSTGDMSVLRHP"
BASE COUNT          637 a         1349 c         1255 g          600 t
ORIGIN      
        1 cagggatccg tcgtcaggcg tcccgactcg aagcgccaac acgggtgccg cgagggccag
       61 cagtgccgag gtcgcgacca cggcgtaggc aacggcgtgc ttggagacgt gggcacccca
      121 gcgatgccac cagccggccg agggcgtccc ggcacggaca gccctggtgc ggtgacgacc
      181 caagatccgt tgcccagcca gaccgaggaa ggcaggcaac agcgtcagcg aggcgagcac
      241 catgatcaag acgatgagcg agatcccggc cccggcagcg gtcacgaacg ggatgccggc
      301 ggccgccagg ccgaggatcg cgatgacgac cgtgccgccg gcgaagagca cggcacgacc
      361 cgaggtcgct gcagcgcgcc cggccgactc gggaacattc atgcccgcgg ccaggttctc
      421 gcgatgccgc gtgagcagga ataatgcata gtcgatcccc accccgagcc cgaccatgct
      481 gccgatcggt gcgacccagc tcggaacgtc gaccaggtag gccagcagcg acatcgacgc
      541 gataccgagg ccaagaccga acacggcgat gccgatcggg aggcccatcg cgatgaacga
      601 gccgaacgcg acaagcagga tgaggacagc agcgagcacc ccgatcagct caccaagtcc
      661 agtcggtggt tcctcgaacg cgaagaagag gtcaccgccg agctcgagct gcagtgggta
      721 cgccgcttcc gcctcggccg cgaaggcctt gaggttctcg aggtcattgg ggctcagatc
      781 ctcgagcacc gagtactgga cgcgcatgac ggcgagcctg ccgtcgggcg agatggcagg
      841 ctcgctcaca ccgagtgcgt gcggcagctc gagagccagc tgacgaacgt cctcgagcgc
      901 agatcgagcg gcagaccccg cgccgaagaa cacaccgtcg tcccgtggcc tcgcaacgac
      961 ctgcgccgtg agaccggcct ggtcggaacc cgccgccgac aacagctcgg tcgcctcctg
     1021 ggagtccagg ccgggagcac cgaaggagtc ctcgagctgc tgaccgaacg cgttggatgc
     1081 tccgagcacg aacaggaagg caacgagcca cgtaccaatg acgacccacg ggcgccgagc
     1141 ggcgaaccgc cccgacagat acagcacgcg cgacagcatc gagtcccctt tcgccatcga
     1201 tcttctgacc ggagtcgaca tctcctgatc gtggcgacag ccagcgcggc atacctccga
     1261 cattgagccg gagtttcttc ggtctcacgg ctgacctgcg agccgtactt tgggctgagc
     1321 cgctcgtcga gcgacttctc tacgctgggc gtgtgacggc caagctcgtc cggcggctct
     1381 acgacgagcc cagagcacct gacccgcctc gacgcgtctg gcgcgactgg gctctcgtcg
     1441 gcgtcatcgt gccgacggcg atcctcgaag gcatcttcag cgaccacgtc ggatggcgtc
     1501 cggtctccgt ggcgatgtgc gtcgcgtttg cgttcacgct gttgtggcga cgcacctacc
     1561 cgctcgctgt cgtcgtgatc accttcgggt ccgtcatcgt gctcgacctc gcctggctga
     1621 tcggtggcgg tggtggcgag ccggtgggcc ttgacaccac ggtgttcatc gtcctgctcc
     1681 cctactcgct ggcgcgatgg ggttctggtc acgacgttgc gatcggcctg ctgttcatgc
     1741 ttgccgccga cgccatggcc caagtcaccg attacaccgg ggtcgccgac ctcgtcttcg
     1801 ggacgctgtt cctcctgttc cctgcagtgc tgggcgcgct ggtccggttc gcggccaccg
     1861 cccaccagcg ggaactcgac cacgtcaggt tccgcgaacg cgagcagttg gcacgcgagc
     1921 tccacgacac ggtcgcccac cacgtctcgg ccatcgccgt ccgggcccag gcgggccggg
     1981 tgctcgccgc gtcgaaccct ggggccgctg ccgacgccct cgacgtcatc gaggaggaag
     2041 cctcccgcac cctcgagcag atgcgtgcca tggtgcgtgg gctgcgcgac agcagcgccc
     2101 catcggtcac accccagccc ggtgtggccg acctcgagcc gctcgcccgc agcgtaggag
     2161 agtcacctcg ggtcgagctg gacctgacgg gcgatctcga cagtctctcg cccgcggtcg
     2221 gagtcgcgat ctatcgcatc gctcaggagt cgatcaccaa tgcagtccgc cacgctcgcc
     2281 acgcgactcg catcgacatc gaggtcgtgg gcgacaccga ctgtgtacgc ctgaccgtgc
     2341 gtgacgacgg cagccctgtc tccgcgagcc cgagcccggc cggctacggc ctggtcggca
     2401 tggccgagcg cgccgcgctg ctggggggct cgctcgaagc cggaccgact tcggatgccg
     2461 gatggacggt tcgggcgatg ttcccgaagg tcgggtccga ccaatgacga tccgggttct
     2521 cgttgccgac gaccaggacc tggttcgcac cgggctggcg atgatcctcc gatcccaagc
     2581 cgacgtcgac gtggtcgcag aagctgccga cgggcgtacc gcggtggcgc tcgcacggca
     2641 gctgcggccg gacgtatgcc tgctggacat ccgcatgccg gacgtcgacg gcatcgaggc
     2701 cacccgccag cttgccggtc cggacgtgga ggatccgctg gcggttgtgg tgatcacgac
     2761 gttcgacctc gacgaatacg ttcacggtgc actcagggcg ggcgcgcgcg gattcctgtt
     2821 gaaggatgcc ggacccgagc tgttgatgca ggcgatccgc gcggcggcca acggtgacgc
     2881 gctcatcgcc ccgagtgtca ccacccggtt gctgacggcg tttgcagacg cgcatcccga
     2941 gcaaccaccg gcgcagccgg tcgaaccgct cacgagtcgc gaggaggaag tcgtgcggac
     3001 cgtggcctgc ggtcgcacca acacagagat cgccgacgag ctccacatct cgctcagcac
     3061 ggtgaagacg catctcgcca gcgtgatggc caagctcggc gcacggaacc gcgtcgagat
     3121 cgccatgtgg gcctacgaga cgcaccgcat cccccgcatc ccgcgctgag cgtggacctt
     3181 gccgcgttcc gctcctgccg atgacactgc acaccgtctc gccaggcccg cccctgcctc
     3241 agggatggcg aaggacgctc atgtccccgg tgctcacaac gtcgtgcgca acgtctcgaa
     3301 ccttacgatt ggagtcctgg ctggccttcg acaagatcgc gaacgccgcc gcctcgctca
     3361 cctgccggct ggccatgaca atgcccatcg ccgccccgat ctggcgcgac gaccgcagcg
     3421 cttcctgcat ctgtgcggca tgctctcggc tgagcacgcc gtcggcctgt aactcggcga
     3481 tcatttcccg atcgacatcg gcacggatct cgagctggtc gattctgagc tgattggcgt
     3541 cgtcacgagc ctcgttctcg tctgccctgt cctcagcagc ttcggcgcgg gtcgccagcg
     3601 cgtcgatgtc ggttcgattg gcggtgaccc gctcgctcag agcgtcgatc tgccgctgta
     3661 cgtccgcatc gcgttcccgc cggtcgtcgt ccatgggaca agtatggcga tggatcccgc
     3721 tcgcccacag gtgccgccgt ttggacgccg atcaggtcag gagccgttcg agcccctggc
     3781 gcccgcgggc gttgaggacc tgcccgaagt acgaccggag gtcatcggcc acaccgaact
     3841 c
//
LOCUS       DATBFZ010000350         1764 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_1551198, whole genome shotgun sequence.
ACCESSION   DATBFZ010000350 DATBFZ010000000
VERSION     DATBFZ010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1551198"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..881)
                     /locus_tag="VH419_10705"
     CDS             complement(<1..881)
                     /locus_tag="VH419_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015069489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEY7044126.1"
                     /translation="MADRIVRGRAPGEPGRVAYLYIAPAFVVFACFMAWPLLRAVWLS
                     FYEWDGLSVGTWVGLDNYREVITNPELREPFLHSLVLLLYFSVLPICLGLGLAWLMTR
                     SQVRGSGFFRTVIFVPQVIAMVVVAIAWRQIYAPDGQLNDFLSTVGLGGLRHAWLGDS
                     TYALSAVGLVGTWVCTGLCMVLFLAGLSKVPKELFEAASLDGAGAVRQFFAVGLPALR
                     GELAVAITLTVVAALRTFDLVYVMTTGGPGTSTRVPSYEVWRWSLDDGRVGYGVTIAI
                     VLTLIIMALTIGVNRFA"
     gene            complement(883..>1764)
                     /locus_tag="VH419_10710"
     CDS             complement(883..>1764)
                     /locus_tag="VH419_10710"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY7044127.1"
                     /translation="SDGDTFGSGNLYGVPLTGEMVGVWYNKAKLDELGIDPPQTVDDF
                     EAALQKAKDAGEIPIQFGDLDQWPGIHEFGFVQNLFVPRDDIIKLGFGQPGGSWTTDE
                     NEQAADTLVDWVNKDYFTDGFNGFGYDPSWQDFAKGKGVFLISGTWLLADLQKAMGDD
                     LGFMLPPTGATGEQAVTGSTGLPFAITEASEHPDVAAAYLDFITSPDAMTKYTEGGDL
                     PVYDTDKQSPTGPQAEVFDAWSQATGDDILTPYLDWATPNSTELVQTQVQELMGDKTS
                     PDDFLDKLESDYKDFTD"
BASE COUNT          309 a          602 c          589 g          264 t
ORIGIN      
        1 tccgcgaacc ggttcacgcc gattgtgagc gccatgatga tgagggtcag cacgatcgcg
       61 atcgtcacgc cgtacccgac ccggccgtcg tcgagcgacc aacgccagac ctcgtacgac
      121 ggcacgcggg tcgacgtccc cggaccgccg gtggtcatca cgtagacgag gtcgaacgtc
      181 cgcagcgccg cgacgacggt cagcgtgatc gcgaccgcga gctcaccgcg cagtgccgga
      241 agtccgaccg cgaagaactg acgcacggcc ccggccccgt cgaggctggc cgcttcgaac
      301 agctccttcg gcaccttcga gagcccggcc aggaacagca ccatgcagag cccggtgcag
      361 acccaggtgc cgaccagtcc gaccgccgac agggcgtagg tcgagtcgcc caaccaggcg
      421 tgccgcagcc cgccgagacc gaccgtcgac aggaagtcgt tgagctgacc gtcgggggcg
      481 tagatctgcc gccaggcgat cgcgaccacg accatggcga tcacctgcgg cacgaagatc
      541 acggtgcgga agaagcccga cccgcggacc tgcgagcgcg tcatcagcca ggccagcccc
      601 agccccaggc agatcggcag cacggagaag tagagcagca gcaccagcga gtgcaggaac
      661 ggctcgcgca gctccgggtt cgtgatcacc tcgcggtagt tgtccaaccc gacccaggtg
      721 ccgaccgaca gtccgtccca ctcgtagaac gacagccaca cggcacgaag cagtggccac
      781 gccatgaagc aggcgaacac cacgaaggcc ggcgcgatgt acaggtaggc cactcgaccg
      841 ggctcgcccg gcgcgcgacc gcgcacgatg cgatcggcca cgtcagtcgg tgaagtcctt
      901 gtagtcgctc tcgagcttgt cgaggaagtc gtccggggac gtcttgtcac ccatcagctc
      961 ctgcacctgg gtctggacga gctcggtgga gttcggggtc gcccagtcga ggtacggcgt
     1021 caggatgtcg tcgccggtcg cctgggacca ggcgtcgaag acctccgcct gcgggccggt
     1081 cggcgactgc ttgtcggtgt cgtacaccgg caggtcgcct ccctcggtgt acttcgtcat
     1141 cgcgtcgggc gaggtgatga agtcgaggta ggccgcggcg acgtccgggt gctcactggc
     1201 ctcggtgatg gcgaacggca agccggtcga gccggtgact gcctgctcgc cggtcgcgcc
     1261 ggtcggcggc agcatgaacc cgaggtcgtc gcccatcgcc ttctgcaggt cggccagcaa
     1321 ccaggtgccc gagatcagga agacgccctt gcccttggcg aagtcctgcc aggacgggtc
     1381 gtacccgaaa ccgttgaagc cgtcggtgaa gtagtccttg ttcacccagt cgaccagcgt
     1441 gtcggcagct tgctcgttct cgtcggtggt ccacgagccg ccaggctggc cgaacccgag
     1501 cttgatgatg tcgtcacgcg ggacgaagag gttctgcacg aacccgaact cgtggatgcc
     1561 cggccactgg tcgaggtcgc cgaactggat cgggatctcg ccggcgtcct tcgccttctg
     1621 cagcgccgcc tcgaagtcgt cgacggtctg cggcgggtcg atgccgagct cgtcgagctt
     1681 ggccttgttg taccagaccc ccaccatctc gccggtcagc ggaacgccgt agaggttgcc
     1741 cgagccgaag gtgtcaccgt ccga
//
LOCUS       DATBFZ010000351         1577 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_2862974, whole genome shotgun sequence.
ACCESSION   DATBFZ010000351 DATBFZ010000000
VERSION     DATBFZ010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2862974"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..591)
                     /locus_tag="VH419_10715"
     CDS             complement(<1..591)
                     /locus_tag="VH419_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014847369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEY7044128.1"
                     /translation="MTTTSQPTKTDTADVSVKTVTLTIDDIEVTVPEGTLVIRAAERI
                     GVQIPRFCDHPLLDPVGACRQCLVEVPDAGNGRPIPKPQASCTLTVAPGMVVRTQHSS
                     KVADKAQQGIMEFLLINHPLDCPVCDKGGECPLQNQAMSNGRGESRYIEPKRTYTKPI
                     HISAQVLLDRERCVLCARCTRFSEQIAGDPFIELVER"
     gene            complement(588..>1577)
                     /locus_tag="VH419_10720"
     CDS             complement(588..>1577)
                     /locus_tag="VH419_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012923849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HEY7044129.1"
                     /translation="SYAIRAHTAFIYVRGEVLHVIRRLQRAVEEAYDAGHLGRDIHGS
                     GFDLDLVVHAGAGAYICGEETALLDSLEGRRGQPRLRPPFPAVAGLYACPTVINNVES
                     IASVPSIIANGSDWFSDLGTEKSKGYGLFSLSGHVTHPGQYEAPLGITLRELLDLAGG
                     VREGHELKFWTPGGSSTPILTAEHLDVPLDFEAVAAAGSMLGTRALQIFDETTCVVRA
                     VLRWTEFYKHESCGKCTPCREGTWWLVQVLERLEAGNGSEEDLDLMIDQCDNILGRAF
                     CALGDGATSPITSSVQYFRDEYLDHLAQGRCPLDPAASALFPTSEPAAAGAAV"
BASE COUNT          250 a          508 c          561 g          258 t
ORIGIN      
        1 acgctcgacc agctcgatga acgggtcgcc ggcgatctgc tcgctgaacc tggtgcagcg
       61 ggcacacagc acgcaacgct cgcggtcgag cagcacctgt gccgagatgt ggatcggctt
      121 ggtgtaggtg cgtttcggct cgatgtagcg ggactcgccg cgaccgttgg acatggcctg
      181 gttctgcagc gggcactcgc cacccttgtc gcagaccgga cagtcgagcg ggtggttgat
      241 caggaggaac tccatgatcc cctgctgcgc cttgtcggcg actttcgagg agtgctgggt
      301 gcgcacgacc atgccgggtg cgaccgtgag tgtgcaggag gcctgcggct tcgggatcgg
      361 ccggccgttg ccggcgtcgg gaacctccac gaggcactgc cgacatgcgc cgaccgggtc
      421 gagcagcggg tggtcgcaga accgcggtat ctgtactccg attcgctcgg cggcccggat
      481 caccaacgtg ccttcgggga cggtgacctc gatgtcgtcg atcgtcaggg tgaccgtctt
      541 gacggacacg tctgccgtgt cggtcttggt cggttgggac gtcgtcgtca tactgcagct
      601 ccagcagcgg ccggctcgga cgtcgggaac agcgccgacg cggccgggtc gagcgggcat
      661 cggccctggg cgagatggtc gaggtactcg tcacggaagt actggaccga ggacgtgatc
      721 ggggaggtcg cgccgtcgcc gagcgcgcag aacgcccggc ccaggatgtt gtcgcactgg
      781 tcgatcatca gatcgaggtc ttcctccgag ccgttgcccg cctccagtcg ctcgagcacc
      841 tgcaccagcc accacgtgcc ttcacggcac ggcgtgcact tgccgcacga ctcgtgcttg
      901 tagaactcgg tccaccgcag caccgcgcgc acgacgcacg tggtctcatc gaagatctgc
      961 agggcgcgag tcccgagcat cgaaccggcg gcggcgacgg cttcgaagtc gagcgggacg
     1021 tccaggtgct cagcggtgag gatcggcgtc gaggacccgc cgggcgtcca gaacttcagc
     1081 tcgtggccct cgcgtacgcc gccggccagg tcgagcagct cgcgcagcgt gatgccgaga
     1141 ggcgcttcgt actggcccgg atgcgtgacg tgaccggaca gcgagaagag gccgtagccc
     1201 ttcgacttct cggtgcccag gtcgctgaac cagtccgacc cgttggcgat gatgctgggt
     1261 acggacgcga tcgactcgac gttgttgatc acggtcggac acgcgtagag cccagcgacg
     1321 gcagggaacg gtggccgcag ccggggctgg ccacgacggc cctcgaggga gtcgagcagc
     1381 gccgtctcct cgccgcagat gtaggcaccc gcgcctgcgt ggacgaccag gtcgagatcg
     1441 aagccggagc cgtggatgtc gcgacccagg tgtccggcgt cgtacgcctc ctccaccgca
     1501 cgttggaggc gccggatgac gtgcagcacc tcgccacgca cgtagatgaa ggccgtgtgc
     1561 gcccggatgg cgtagct
//
LOCUS       DATBFZ010000352         3701 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_6030506, whole genome shotgun sequence.
ACCESSION   DATBFZ010000352 DATBFZ010000000
VERSION     DATBFZ010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 3701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3701
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6030506"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..633
                     /locus_tag="VH419_10725"
     CDS             <1..633
                     /locus_tag="VH419_10725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEY7044130.1"
                     /translation="DDYTLLRMPSRMLNTKARLVLRFLGDKPPHIAVGGRQVSMTMRR
                     AELLTLLAASPAGMSADTLTLQLYGDEGNSTTTRAELHRVRQILGDRISAKPYRLTGP
                     FSADFLDVREHVLRGRVDDALRLYAGPLLPGSTSLAVELLRDELHAMMRRAVIQAGRK
                     TLEEWVESPHGAGDLDAICALLDLLPGYDHRRPVLQARADRLASEYGVPG"
     gene            complement(642..1271)
                     /locus_tag="VH419_10730"
     CDS             complement(642..1271)
                     /locus_tag="VH419_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit B"
                     /protein_id="HEY7044131.1"
                     /translation="MAWTREQMAARAAGELEDGAYVNLGIGLPTLVPNYVPDDVEIVL
                     QSENGILGVGAYPYDGEEDPDLINAGKETVTVRPGAAFFDSATSFGMIRGGKIDAAIL
                     GAMQVSVNGDIANWMIPGKMVKGMGGAMDLVHGAKRVIVLMEHVARDGSYKVVNDCSL
                     PYTGRGVVQRIITDLCVLDVTDDGLQLMELAPDVTEDEVREKTEPPVRT"
     gene            complement(1271..2053)
                     /locus_tag="VH419_10735"
     CDS             complement(1271..2053)
                     /locus_tag="VH419_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HEY7044132.1"
                     /translation="MDKVVASAAEAVADIESGSSLAVGGFGLCGIPTVLIQALLDAGI
                     TDLEAVSNNCGVDDWGLGLLLREKRIRRMVSSYVGENKEFERQYLHGELEVELTPQGT
                     LAERLRAGGAGIPAFYTATGVGTQVAEGGLPWRYDDQGNVVTSSPPKETRRFDTAEGP
                     KDFVLEPAIVADYALIRAWRGDRHGNLVFRDSARNFNPLCAMAGRVTIAEVEELVEPG
                     QLPSNDVHLPGVYVQRVVPLTAEQAREKRIEKRTVRPREGQS"
     gene            2171..3229
                     /locus_tag="VH419_10740"
     CDS             2171..3229
                     /locus_tag="VH419_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017933306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HEY7044133.1"
                     /translation="MTPPAASHPDTPVPRLHIVTGKGGTGKTTVAGAFALALAGVGHK
                     VLLCEVEGRQGIASLFDVAPLPYGERRIAVGSDGGEVFALAIDPEDALMEYLDMFYRL
                     GRAGKALDRFGVIDFATTVAPGVRDVLLIGKVYEATRRGVSTRQRRGGRHAQTAYDAV
                     VLDAPPTGRIGRFLNVNTEVAGIAKVGPVRNQATSIMRLLKSDVTSVHVVTSLEEMPV
                     QESLDAIDELRAIDLHVGTLVVNLQWETELSARDLKAAAAGKLDTAEIEKGLKEAGLA
                     SDEKVVQGLVAEAGDHAVRVSLERTQRRRLQAVGLPMVTLPYVSDGVDLGTLYDFADI
                     LRSAGVDHVEYGQHGVRS"
     gene            3301..>3701
                     /locus_tag="VH419_10745"
     CDS             3301..>3701
                     /locus_tag="VH419_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009084488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HEY7044134.1"
                     /translation="MGALTGRLADVPALDVEGLIRDDAVRIVVCCGSGGVGKTTTAAA
                     LALRAAELGRKAVVLTIDPARRLAQSMGLTELDNTPRAVTDADTAVGGSLDAMMLDMK
                     RTFDEVVEAHASREKAAQILANPFYQALSSS"
BASE COUNT          576 a         1287 c         1263 g          575 t
ORIGIN      
        1 gacgactaca ccctgctccg gatgccgagc aggatgctca acacgaaggc ccggctcgtc
       61 ctgcggttcc tcggagacaa gccgccgcac atcgcggtcg gtggtcgcca ggtctccatg
      121 acgatgcgcc gtgccgagct cctcaccctg ctcgcagcaa gtcccgccgg gatgagcgcc
      181 gacacactga ccctccagct gtacggcgac gagggcaact cgacgacgac ccgcgccgag
      241 ctgcaccgcg tccggcagat cctgggcgac cgcatctccg ccaagcccta ccggctcacc
      301 ggcccgttca gcgcggactt cctcgacgtg cgggagcacg tgctgcgtgg gcgggtcgac
      361 gatgcgctgc ggctgtacgc aggcccactc ctcccgggct ccacctcgct ggccgtcgag
      421 ctgctgcgcg acgagctgca cgcgatgatg cgccgcgcgg tgatccaggc cggtcgcaag
      481 accctcgagg agtgggtcga gtcaccgcac ggcgccggcg acctggacgc gatctgcgca
      541 ctgctcgacc tgctgccggg atacgaccac cggcggccgg tcctgcaggc gcgcgccgac
      601 cggttggcgt cggagtacgg agtccccggc tgaggtcagg ttcaggtgcg gacgggcggc
      661 tcggtcttct cccggacctc gtcctcggtg acgtcggggg cgagctccat gagctgcagg
      721 ccgtcgtcgg tcacgtcgag gacgcagagg tcggtgatga tgcgctgcac gacgccacga
      781 ccggtgtagg gcagcgagca gtcgttgacg accttgtacg aaccgtcgcg cgcgacgtgc
      841 tccatcaaca cgatgacccg cttggcgccg tgcacgaggt ccatcgcgcc gcccatgccc
      901 ttgaccatct tgccggggat catccagttg gcgatgtcgc cgttcaccga cacctgcatc
      961 gcgccgagga tggccgcgtc gatcttgccg ccgcggatca tgccgaacga cgtcgccgag
     1021 tcgaagaacg cggcaccggg ccggacggtc accgtctcct tgccggcgtt gatcaggtct
     1081 gggtcttcct cgccgtcgta cggatacgcg ccgacgccga ggatgccgtt ctcggactgg
     1141 aggacgatct cgacgtcgtc ggggacgtag ttcgggacca gtgtcggcaa cccgatgccg
     1201 aggttgacgt acgccccgtc ctcgagctca cccgccgccc gcgcggccat ctgctcgcga
     1261 gtccacgcca tcagctctgt ccctccctcg gccgcacggt gcgcttctcg atccgcttct
     1321 cgcgcgcctg ctcagcggtg agcggtacga cgcgctggac gtacacgccg ggcaggtgga
     1381 cgtcgttcga cggcagctgg ccgggctcga cgagctcttc cacctcggcg atcgtcacgc
     1441 ggcccgccat cgcgcacagc gggttgaagt tgcgcgccga gtcgcggaag accaggttgc
     1501 cgtgccggtc accgcgccag gctcggatca gcgcgtagtc ggcgacgatc gccggctcga
     1561 gcacgaagtc cttcgggcct tcggcggtgt cgaagcgacg cgtctccttc ggtgggctcg
     1621 atgtgacgac gttgccctgg tcgtcgtacc gccacggcag accgccctcg gcgacctgcg
     1681 tcccgacgcc ggtcgccgtg tagaaagccg ggatacctgc gccaccggcc cggagccgct
     1741 cggcgagggt tccctgcggc gtcagctcca cctcgagctc accgtgcagg tactgacgct
     1801 cgaactcctt gttctccccg acgtaggacg aaaccatccg acggatgcgc ttctcccgca
     1861 gcagcaaccc gagaccccag tcgtcgaccc cgcagttgtt ggagaccgcc tctaggtcgg
     1921 tgatgccggc gtccaagagc gcctggatga gcacggtcgg gatcccgcac aagccgaaac
     1981 caccgaccgc gagcgacgag ccgctctcga tgtcggcgac ggcctcggct gcgctcgcga
     2041 cgaccttgtc cacgtccacc acctccctga cgcccggcgg atccggcccc tcgaaaccta
     2101 ctgcatgcag ccttcgttca tgcctccggc caagaggtgc acctggggct gcgcggctac
     2161 cctcgaaacc gtgactcccc ccgccgcgtc gcacccggac acgcccgttc cgcggttgca
     2221 catcgtcacg gggaagggcg gaacgggcaa gacgaccgtg gccggagcgt tcgcgctggc
     2281 gcttgcgggc gtaggccaca aggtcctgct ctgcgaggtc gaggggcgac agggcatcgc
     2341 gtcgctgttc gacgtcgcac cattgccgta cggcgagcgc cgaatcgctg tcgggtcgga
     2401 cggcggtgag gtcttcgcac tcgcgatcga tcccgaggac gccctcatgg agtacctcga
     2461 catgttctac cggctcggtc gtgccgggaa ggcgctcgac cggttcggtg tgatcgactt
     2521 cgcgacgacg gtggcaccgg gcgtgcgtga cgtgctgctg atcgggaagg tctacgaggc
     2581 cacgcgacga ggcgtgtcga ccaggcaacg gcgcggcggc cgacatgcac agacggcgta
     2641 cgacgctgtg gtcctcgacg cgccaccgac cggccggatc ggacgctttc tcaacgtcaa
     2701 caccgaggtc gccggtatcg cgaaggtcgg accggtccgc aaccaggcca cgtcgatcat
     2761 gcgcctgttg aagtccgacg tgaccagcgt gcatgtcgtc acctcgctgg aagagatgcc
     2821 cgtccaggag tcattggatg cgatcgacga gcttcgagcg atcgatctgc acgtgggaac
     2881 gctggtggtc aacctgcagt gggagacgga gctgtccgca cgcgacctga aggctgcggc
     2941 ggccgggaag ctggacaccg ccgagatcga gaagggcctg aaggaggcgg gtctcgcctc
     3001 ggacgagaag gtcgttcagg gtctcgtcgc ggaggcaggt gaccacgcgg ttcgggtcag
     3061 cctggagcgc acgcagcgtc gacgcctgca ggcggtgggt cttcccatgg tgacactgcc
     3121 gtacgtctcg gacggtgtcg atctcggaac gctctacgac ttcgccgaca tcctccggtc
     3181 ggcgggcgtc gaccatgtcg agtacggcca gcatggggtg cgcagctgat ggcgccgcca
     3241 cggcagtccc ggacaggccg gcgtccgaag acgcagccag gccgcggcaa ccagccgcgc
     3301 gtgggagcgc tgaccgggcg gctcgccgac gtccccgcgc tcgatgtcga agggctcatc
     3361 cgcgacgacg cggtccggat cgtggtctgt tgcggctccg gaggggtcgg caagaccacg
     3421 accgccgctg cgctcgcgtt gcgcgcggcc gagctcggcc ggaaggcggt ggtgctcacc
     3481 atcgatccgg ctcggcggct ggcccagtcg atggggctca ccgaactcga caacacaccg
     3541 cgcgcggtga cggacgccga caccgcagtc ggcggaagcc tggacgccat gatgctcgac
     3601 atgaagcgga cgttcgacga agtggtcgag gcgcacgcat cgcgggagaa ggcggcgcag
     3661 atcctcgcga accccttcta ccaagcactg tcgagctcgt t
//
LOCUS       DATBFZ010000353        18430 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_4176397, whole genome shotgun sequence.
ACCESSION   DATBFZ010000353 DATBFZ010000000
VERSION     DATBFZ010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 18430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18430
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4176397"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="VH419_10750"
     CDS             complement(<1..351)
                     /locus_tag="VH419_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020578630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HEY7044135.1"
                     /translation="MRQAHTVHDIRAAEKSLMAALPPGTLMQRAAAGLATVCAQLLSD
                     GPGSYGSRVLVVAGGGDNGGDALFAGAALARRGVRVEALLVGSHAHEAGLAALRRCGG
                     YVVQARDNRYDLVVD"
     gene            complement(361..711)
                     /locus_tag="VH419_10755"
     CDS             complement(361..711)
                     /locus_tag="VH419_10755"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007078589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEY7044136.1"
                     /translation="MIVGVGVDVVDVERFGETLRRTPAMRDRLFTDTELGRPLASLAA
                     RFAAKEALAKALGAPSGMHWLDAEVQTDETGRPSMALTGSVARRASEVGAARVHVSLS
                     HDAGIASAVVVLED"
     gene            833..1780
                     /gene="coaA"
                     /locus_tag="VH419_10760"
     CDS             833..1780
                     /gene="coaA"
                     /locus_tag="VH419_10760"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEY7044137.1"
                     /translation="MASDDVSPYVELDRSAWAALAESMPLPLSAEEVERLRGLGDELD
                     LEEVADVYLPISRLLSLYVRHVGALHEATEAFLGARQPKRTPFVIGIAGSVAVGKSTT
                     ARLLRELLAHWPEHPNVALVTTDGFLYPNAELERRGLLNRKGFPESYDRRSLLKFVID
                     IKSGRDEVSAPVYSHLEYDVIPGKHLTLHHPDIVLIEGLNVLQAARVTGDGRIGLAVR
                     DFFDFSVYVDAATPDIRQWYVERFLRLRETAFRDPASYFRRYAHLSEKEAVRQAESIW
                     DAINGPNLDGNIKPTRGRATLVLRKDADHSVRYVRLRKL"
     gene            1954..2988
                     /locus_tag="VH419_10765"
     CDS             1954..2988
                     /locus_tag="VH419_10765"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY7044138.1"
                     /translation="MKIRRIDLHDDALLHQYYVTMRAADLYERERMPYWSEHAAAVMF
                     RREEPGEQWHFFAAFDGEGDDEHIVGTAYMVLPLSDNLQFAFLGVAVAPENRCRGIGS
                     ALAEYVVAEARKTGRTRLTGEMNIPFEEREAHPYRRFAERHGFSLANVEISRILELPV
                     SDDQLAEWIDESAPHHPGYRIETFVDEIPKELVPSLVHVRNQLAADAPTGDLDFEAEK
                     MTPEEFEIRREKAKEMGRTIFETVAIDDSDGTGKVVAHSTLAVPNDDPGNVYQWGTLV
                     LREHRGHRLGMAVKARNLRAVQQAYPDRKRVITSNAEVNAPMVSINERMGFKPVELMA
                     EFQRTYDEPA"
     gene            2992..3996
                     /locus_tag="VH419_10770"
     CDS             2992..3996
                     /locus_tag="VH419_10770"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY7044139.1"
                     /translation="MLTIRRVDPEHDETMTAYSSICIESESFEDPYATPFTYVESREA
                     LRNTDPSDVCEGYLGYVDDRPVATGTVFLFLSDNTDKSFVFVNTLPEFRNRGHGSQML
                     DFALERLRTEGRTTALSEATYPFEADESHPYRRFLTDRGFEFSQAEVHRVLELPPDAE
                     LLDKLKADAAPHHADYTLADYTGLVPESMREDYCVLLNQIIVDAPSGTIDFEEGRLTP
                     EMLAEKEESSRARGRTTYVTVALDRDGVPVAHNVLVVPETDPGKIFNHDTLVRRDHRG
                     HRLGLATKIHNLRRVAALHPDRTTVHTWNAESNAPMIAVNDAMGFTPVRYGGAYHRPL
                     "
     gene            complement(4116..4751)
                     /locus_tag="VH419_10775"
     CDS             complement(4116..4751)
                     /locus_tag="VH419_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY7044140.1"
                     /translation="MITVVLADDHALVRRGLERLLDADPGIEVVGTAADGVEAVTLVE
                     AKRPDVVLMDLQMPQLDGVEATRRIVRDSPDDKQVQVVVLTSFSDTERIVAALDAGAI
                     GYLLKDTEPEDLLEGVRTAARGESPLHPRAARQLLTARTKQQPTTTVDLTKRETEVLG
                     LVRQGLANKQIARRLGISERTVKAHLTSTFQRIGVTDRTQAALWAERHGIN"
     gene            complement(4748..6151)
                     /locus_tag="VH419_10780"
     CDS             complement(4748..6151)
                     /locus_tag="VH419_10780"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEY7044141.1"
                     /translation="MPGARQSTDPVSTSPAPGPQASTRFGSAVAPPRAASAVDTLRRF
                     VSNPVVQFLAAGLVALLLLLAVSGWLSQRAATDEAITDARATTQLLGRSAVEPTLTRG
                     VVDQNAAALDKFDRQMRKRVMGGDILRIKIWDRDGTILYSDDPRLIGDTFELEPAEVA
                     IIDDGGTAAEVSDLNKAENKFERGNGRLLEVYSQIWAPGGKPLLFEAYFSYDDVSDRS
                     AQVLSAFRPIILAGLLIFLAMTAPLVYVLARRLDAAAANRERLLLAAVRASDTERRRI
                     ARDLHDGVVQQLAGTSFELSAAARDSRGEPERARRLEEIAGGVRHSLRSLRSLLVEIY
                     PPELQTEGLAAAFDDLVSPCIAAGIAVDVQVADHSDVPPEVIALVWRVGQEAVRNAQR
                     HGRPTRLAVIVTRPEGGIKLEVVDDGVGFDTSAPPPTDHLGLRGLRDLVRDVGGILDV
                     QSAPGAGTRVTLEVRRS"
     gene            complement(6208..6651)
                     /locus_tag="VH419_10785"
     CDS             complement(6208..6651)
                     /locus_tag="VH419_10785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044142.1"
                     /translation="MKTTWEVERNRGRLRRSRRRVWTPAVPVAAVAMAIAAGGLTPAD
                     ANDRDVIRRGSCSGHAEWKLKASPEDGRIEVEAEVDSSRNGQVWKWRILHNGDVSAHG
                     TRKTKPPSGSFEVRRLMLNLAGTDTIGWRARQPASGQVCRGSLRF"
     gene            complement(6750..7751)
                     /locus_tag="VH419_10790"
     CDS             complement(6750..7751)
                     /locus_tag="VH419_10790"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY7044143.1"
                     /translation="MQVREIDVHDDDEVARWWEVARSADAHGRDYAAYWSLRAAMVAL
                     RAENSPFRQQPIAAFDRAGMVGVDKLMLPLADNTHMAFVAPMVLPEHRRRGVGSALLE
                     TSLDMARVAGRTTAVTEVNLPLDPEAESAGLTFTRRHGFELGILDIHRVLDLPVSDRR
                     LDELTAAAAPHHHAFRLSTWIDAVPEEYMDGFCELQIAFNSEAPMGELELENEVWDAE
                     RVRSTEERFRKQGRHEVSTVAIASDGTLVGLTQLMTTEENRAVAWQGGTLVLKSARGH
                     RLGIAMKAQNLRRFQPLFPDSTVVHSWNAEENGPMVAINETLGFRPVERLAEMQKKL"
     gene            complement(7803..9152)
                     /gene="glmM"
                     /locus_tag="VH419_10795"
     CDS             complement(7803..9152)
                     /gene="glmM"
                     /locus_tag="VH419_10795"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002514806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEY7044144.1"
                     /translation="MVSRLFGTDGVRGVANRELTAELALDLSVAAARTLGQSGFRSRR
                     RPVAVVGRDPRASGEFLEAVVVGGLASAGVDVRRLGVLPTPAVAYLTAEMGADFGVML
                     SASHNPMPDNGIKFFARGGHKLEDGVEDAIEQAMGQPWDRPTGAEVGRVTDDPDAVGR
                     YVDHLASTVTSSLAGLSFVLDCAEGAASATAPHVFRALGAEVRTIHDQPDGFNINVRC
                     GSTHIAGLQRAVLEHGADAGFAFDGDADRCLAVDQSGEVVDGDQILAILALALRDSGA
                     LPGNTVVATVMSNLGFVLAMREAGVGVVTANVGDRYVLEEMRSGGYVLGGEQSGHVIM
                     STYGTTGDGVLAAVHVAERMASQRRSLADLAGLVQRLPQTLVNVPDVDRARVTTDATV
                     QSAVRAAEAELGETGRVLLRPSGTEPLVRVMVEAPSAEQADAVAHRLADVVKASLAL"
     gene            complement(9186..9692)
                     /gene="rpsI"
                     /locus_tag="VH419_10800"
     CDS             complement(9186..9692)
                     /gene="rpsI"
                     /locus_tag="VH419_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEY7044145.1"
                     /translation="MAETTNETVDIADPVDTDAPSEYTTESAPAADVAARAVTIAAAG
                     ATGRRKEAVARVRLVPGTGAWTVNGRPLEAYFPNKLHQQLVNEPFVLTQLVGRYDVIA
                     RIHGGGLTGQAGALRLGVSRALNDSDTESNRSTLKKAGMLTRDARIKERKKAGLKKAR
                     KAPQYSKR"
     gene            complement(9751..10194)
                     /gene="rplM"
                     /locus_tag="VH419_10805"
     CDS             complement(9751..10194)
                     /gene="rplM"
                     /locus_tag="VH419_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016473395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEY7044146.1"
                     /translation="MRTYSPKPGDVTREWHVIDATDVVLGRLAVQAATLLRGKHKTMY
                     APHVDTGDFVIIVNADKVALSGNKRATKLAYRHSGYPGGLTATTYGELLDKDPRKAIE
                     KAVWGMLPKNRLGRQMLKKLKVYGGPDHPHAAQQPKPFDIVQIPQ"
     gene            10490..11845
                     /locus_tag="VH419_10810"
     CDS             10490..11845
                     /locus_tag="VH419_10810"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007078596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HEY7044147.1"
                     /translation="MSSDSHEAATSAGNATAESGSAGGATLTVRDNRTGAEYDLPILD
                     GTVRASDLAKIRLKDDEPGIAVYDPGFVNTASCRSSITYIDGDKGILEYRGYPIEQLA
                     EKSTYLEVAYLLIHGELPTKQQFEAWEHQITFHTFVHENVKSFMEGFRYDAHPMGMLL
                     ASVGALSTFYPESRNIADPNVRQMQIIRLIAKMPTLGAWAFRHAQGKPYIYPDNELGY
                     AANFLAMLFRMSEEQFEADERLIRALEVLFILHADHEQNCSTNAVRSVGSSQVDPYSA
                     VSAGIAALFGPLHGGANEAVLKMLRRVGSVDNVPEFIDGVKNGKERLMGFGHRVYKNY
                     DPRATIIKKACDDVFEVTGVNPLLKIAVELEKIALEDEYFVKRKLYPNVDFYSGLIYE
                     ALSFPPEMFTVLFAIPRTSGWLAQWLELTEDSEQKIARPKQIYTGQRKLDFVPSSDRW
                     S"
     gene            12380..13873
                     /locus_tag="VH419_10815"
     CDS             12380..13873
                     /locus_tag="VH419_10815"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY7044148.1"
                     /translation="MTSIWMDRPGLASHPPLTGDATYDVAVVGGGLTGLVTAVLFARS
                     GRRVVLLEARRLAAVTTGNTTGKISLLQGTKLSRLAKRHPADVVRAYVDAGREGQQWL
                     LRYCDEHGVAVQTRPAYTYATTAEGEQAAREELETARRAGLDAEWVETTALPFDVRGA
                     VRLADQAQFDPMDVVAALADDLVERSGVIHEESPVTSLRRGDGGVRIAAPTATERAET
                     VVVATGTPIFDRGGYFARLEPDRSYAVAFRSPGTPPAGMYLSADTPTRSLRSYPREDG
                     EYLLAGGNGHVVGRRSATTQQYDDLVRWTESMFPGAQPTHRWSAQDYRSVDGLPYVGP
                     LTPGHDDVLVATGYDKWGMTTAVAAALALSARVLGGQTPWADAMDSWRLRELGGLPTA
                     LRLNASVALHLASGYLQAFGHGGSEIGPAEGEGRVFRRAGRPTAACRVDGVTHELSAV
                     CPHLGGILTWNDAERSWDCPLHGSRFAADGTRLEGPATKGLPPAVPA"
     gene            13901..15556
                     /locus_tag="VH419_10820"
     CDS             13901..15556
                     /locus_tag="VH419_10820"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HEY7044149.1"
                     /translation="MRSRLRRLVDRSMGKLTGGRSSRQVRRTAYGSSGDLAPGLFVNP
                     IAEGADPDVVKDGERFLWCQAEGNVGISIWTSARLTSLGTKHVVWRAAADGPCSKEVW
                     APELIRLDGRWHVYFAASDGRNRNHRTFVLVADTDDPLGSYTLEGPLFTGDQPGGDNL
                     WSIDFTVLEHDERRFGLWSGWPDADTDRQDLYIAEMSSPTDVKGGRVRIAEAGVHDWE
                     RIYESPKSRGLLEGPRVLQRDGRTFVLYSCAASWLPTYKLGVLELTGDDPMSPSSWAR
                     WGRPVFASTDETYGVGHSSCVHFGQIGSADEEWWHVFHSKVDRRDGWRRALHVQPMEW
                     RHDGTPMLGEPLPPGQALTMPRAYRAQLRHAGGSWDFGVDGLDDFDYYGHHQYVAVGP
                     EGLDLGVVPDTPVNHFRSGEKVVLRGGDFSDVEIEATWTFVSSTRSAGVLFRTTGAAV
                     GYDAQRSYFAAIVPKEDTLVIGKTDGSTWTRMATTKMPVRVDRPQTLRVSAVGTHLVV
                     TAGGSTIELDDSDHARGSVGLRTVNTHARFRSLTVSPLPTSAS"
     gene            15567..16193
                     /locus_tag="VH419_10825"
     CDS             15567..16193
                     /locus_tag="VH419_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008361082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEY7044150.1"
                     /translation="MAAVDAVVFDLGNVLIEWDPHPAIAAGVGADEASRFLTATDFDF
                     GSWNHQQDSGTPWVDAELAVEKSHPHWHRHATAYRQHFHRSLLGAIPGTVDILRELHA
                     AGTAVYGLTNWSAELFPVARESYAFLDLFDDIVVSGEEGVAKPDPGIFDVLRERVGRP
                     LDACLFVDDNPANVDAARTAGMDAIVFTGPVALRAELASRDLLGAIDG"
     gene            complement(16287..17969)
                     /locus_tag="VH419_10830"
     CDS             complement(16287..17969)
                     /locus_tag="VH419_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002527553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY7044151.1"
                     /translation="MGHVDVAGVRYELPDGRVLLDDVAFRVGEGAKVALVGANGAGKT
                     TLLRIVTGELTPHAGTVTRSGGLGVMRQLVTQGTDGLTVHDLLLSVAPPRVRQAAAHV
                     EAAELSMMEHDDEKSQMAYAEALAEWSDAGGYDSEVLWDVCTIAALGVSYDKAKYRDL
                     TTLSGGEQKRLVLEALLRGPDEVLLLDEPDNFLDVPGKLWLEERIRESPKTILFVSHD
                     RELMNNTATRIVTVELGAAGNTVWIHPGGFATYHEARRDRFARLEELRRRWDEEHAKL
                     KALVLRYKIKAEYNDGMAAQYKAAQTRLRKFEEKGPPQAIPREQNVTMRLTGGRTGKR
                     AVICEALELTGLMQPFDLEVWYGERVAVLGSNGSGKSHFLRLLAAGGSDPDVEHRPVG
                     EVPIPPVDHAGRARLGARVRPGWFVQTHEHPELIGRTLLEILHRGDHHRAGMPREQAA
                     RALDRYELAHASEQRFESLSGGQQARMQILLLELSGATLLLLDEPTDNLDVESAEALE
                     DGLESFEGTVVAVTHDRWFARGFDRFMVFGADGSVYESDQPVWDEGRVQRTR"
     gene            complement(18142..>18430)
                     /locus_tag="VH419_10835"
     CDS             complement(18142..>18430)
                     /locus_tag="VH419_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020386123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY7044152.1"
                     /translation="RDAGIDVRTGHVDLTTSARGWIHKVGNDGFIKLVEDADQGLLVG
                     ATSAGPNGGEVLSALVVAIHGEVPTASLRSMIYAYPTLHRAIEVAVNDLDS"
BASE COUNT         2988 a         6419 c         6064 g         2959 t
ORIGIN      
        1 gtcgacgacc aggtcgtacc gattgtcgcg agcctggacg acgtacccgc cgcaccgacg
       61 cagcgcggcg agccccgcct cgtgcgcgtg tgaaccgacg agcagggctt cgacgcgcac
      121 gccgcgtctg gccagcgcgg cgcccgcgaa caacgcgtca ccaccgttgt ccccgccacc
      181 ggcgaccacc aggacccgtg aaccgtagct gcccggcccg tcggagagca gctgcgcgca
      241 caccgtggcc aagcctgcgg ccgcccgctg catcagcgtc ccgggcggca gtgccgccat
      301 cagggacttc tccgccgccc ggatgtcgtg gacggtgtgc gcctgccgca tgtgacctcc
      361 tcagtcctcc aggacgacca ccgcggaggc gatgccggcg tcatgggaga gcgagacgtg
      421 tacccgggca gcccccacct ccgacgcacg acgcgcaacc gaaccggtca gcgccatcga
      481 gggccgaccg gtctcgtccg tctgcacctc ggcgtccagc cagtgcattc ccgacggtgc
      541 gccgagcgct ttcgccagcg cctccttggc cgcgaagcgt gccgccagag aggcgagcgg
      601 ccgcccaagc tcggtgtcgg tgaacagccg gtcgcgcatc gccggtgtgc gccgcaaggt
      661 ctcgccgaag cgttcgacgt cgacgacgtc gaccccgact ccgacgatca tccgcgcctc
      721 ctcggcatcc acggcgtacc tcacgctgcc ggacccggcg ctcagcctaa cggcccgtcg
      781 gcacccctgc cctggcgagc aaccgcgact cctgcaggcg acaatggcgc gcatggcatc
      841 cgatgacgtc tcgccctacg tcgagctgga ccgctccgcg tgggccgcgc tggcggagtc
      901 gatgccactt ccgctctcgg ccgaggaggt cgagcggctg cggggcctcg gcgacgagct
      961 cgacctcgag gaggtcgccg acgtctactt gccgatctct cgtctgctca gcctctacgt
     1021 ccgccacgtc ggtgcgttgc acgaggcgac cgaggcgttc ctcggtgctc gccagcccaa
     1081 acgcacgccg ttcgtgatcg ggatcgccgg gtcggtggcc gtcgggaagt cgacgaccgc
     1141 ccggttgctg cgtgagctct tggcgcactg gcccgaacac cccaacgttg cgctggtgac
     1201 gaccgacggc ttcctgtatc cgaacgccga gctcgaacgc cgcggcctgc tcaaccgcaa
     1261 gggcttcccg gagtcgtacg accgccgctc gttgctcaag ttcgtcatcg acatcaagtc
     1321 cgggcgggac gaagtcagcg ccccggtgta ctcgcatctc gagtacgacg tgataccagg
     1381 caagcacctc accctgcacc accccgacat cgtgctcatc gagggcctca acgtcctgca
     1441 agcggctcgg gtcaccggcg acgggcggat cggcctggca gtgcgggact tcttcgactt
     1501 ctcggtgtac gtcgacgcgg cgacgccgga cattcgccag tggtacgtcg agcggttcct
     1561 gcgactgcgt gagaccgcct tccgcgaccc ggcgtcgtac ttccgtcggt acgcccatct
     1621 gagtgagaag gaggccgtcc ggcaggcaga gagcatctgg gacgccatca acggacccaa
     1681 cctggacggc aacatcaagc cgacccgcgg ccgcgccacc ctggtgctcc gcaaggacgc
     1741 cgaccactcc gtccgctacg tccgcctccg caaactctga cccgggacgg gtttgcgatg
     1801 tttacagccc aggcggcgtg taaccatcgc agacctgcgg tgggcagggt gggtttgcga
     1861 tcctcacatc gcaaacccaa cgggcctgcg gcgagggcgc ggaggacgga aaaccagtag
     1921 tggacggtcg gtcgttctcc ctaccctcgg aacgtgaaga tccggcgaat cgacctccac
     1981 gacgacgcgt tgttgcatca gtactacgtg accatgcgcg ccgccgacct ctacgagcgt
     2041 gagcgcatgc cgtactggtc cgagcacgcc gcggctgtca tgttccggcg cgaggagccc
     2101 ggcgagcagt ggcacttctt cgccgccttc gacggggaag gcgacgacga gcacatcgtc
     2161 ggcaccgcgt acatggtcct gccgctgagc gacaacctcc agttcgcctt cctcggcgtc
     2221 gccgtcgccc ccgagaaccg gtgtcgcggt atcggttcgg ccctcgcgga gtacgtcgtg
     2281 gccgaggccc gcaagaccgg ccgcacgagg ctcaccgggg agatgaacat cccgttcgag
     2341 gagcgcgagg ctcatcctta ccggcgattc gccgaacggc acggcttcag cctggcgaat
     2401 gtcgagatca gccggatcct cgagctgccc gtcagtgacg accagctggc cgagtggatc
     2461 gacgaatccg ccccgcatca cccgggctac cggatcgaga cattcgtcga cgagattccg
     2521 aaggagctgg tgccctcgct cgtccacgtc cgcaaccagc ttgcggccga cgcgccgacc
     2581 ggtgacctcg acttcgaggc ggagaagatg acgccggagg agttcgagat ccgccgggag
     2641 aaggcgaagg agatggggag gacgatcttc gagaccgtcg ccatcgacga cagcgacggc
     2701 accggcaagg tcgtggcgca ttcgacgctc gccgtcccga acgacgaccc cgggaacgtc
     2761 taccagtggg gcacgctcgt cctgcgcgag caccgcggcc accgtctcgg catggcggtg
     2821 aaggcccgca acctgcgtgc ggtgcagcag gcgtatccag atcgcaagcg cgtcatcacg
     2881 agcaacgccg aggtgaacgc gccgatggtg tccatcaacg agcggatggg cttcaagccg
     2941 gtcgagctga tggcggagtt ccagcgcaca tacgacgagc cggcgtgaac catgctgacg
     3001 atccggaggg ttgatcccga gcacgacgag acgatgacgg cgtactcgtc gatctgcatc
     3061 gagagcgagt ctttcgaaga cccgtatgcg acaccgttca cttacgtcga aagccgggaa
     3121 gcactccgta acaccgaccc ttccgacgtc tgcgagggct atctcgggta cgtcgacgac
     3181 cgtcccgttg cgacggggac ggtgttcctg ttcctgtctg acaacaccga caagagcttc
     3241 gtcttcgtca acacgcttcc cgagttccgt aaccgcggcc atggctcgca gatgctggac
     3301 ttcgccctgg agcgactgcg gacggaagga cgcaccacgg cgttgagcga ggcgacgtac
     3361 ccgttcgagg cagacgagtc ccacccgtac cgacggtttc tgaccgaccg agggttcgag
     3421 ttctcgcagg ccgaggtgca ccgcgtcctc gagcttcctc cggacgcgga actgctcgac
     3481 aagctcaagg cagacgccgc tccgcaccac gccgactaca cgctcgccga ctacaccggg
     3541 ctcgtcccgg agtcgatgcg cgaggactac tgcgtcctgc tcaaccagat catcgtcgac
     3601 gccccaagcg gcaccatcga cttcgaggaa gggcgcctca ctcccgagat gctggcggag
     3661 aaggaagagt cctcacgcgc gagaggccgg acgacatacg tcacggtcgc gttggaccgc
     3721 gacggcgtac cggtcgcaca caacgtcctg gtcgtcccgg agaccgaccc cggcaagatc
     3781 ttcaaccacg acacgctcgt acgccgcgac caccgtgggc accgactcgg actcgcgacg
     3841 aagatccaca acctccgacg tgtcgccgca ctgcacccgg atcggacgac cgtccacacc
     3901 tggaacgccg agtccaacgc accgatgatc gccgtcaacg acgccatggg cttcacgcca
     3961 gtgcgctacg gaggcgccta ccaccgcccc ctctgaccgt tcctggtggc tcccgcgaat
     4021 tgcgggaaac gtcagggtca gacgggcgtg tggaccgcac ctttcccgca atcggcagag
     4081 acccctggcg ggccgaccag tcgcaggttg ctccgctagt tgatgccgtg ccgctcggcc
     4141 cacaaggcgg cctgcgtgcg atcggtcacg ccgatgcgtt ggaacgttga cgtcaggtgt
     4201 gccttgacgg tccgttcact gataccgagg cgccgggcga tctgcttgtt ggccagtccc
     4261 tggcggacca gccccagcac ctccgtctcg cgcttcgtca ggtcgaccgt cgtcgtcggc
     4321 tgctgcttgg tccgcgccgt gagcagctga cgggccgccc gcggatgcag cggtgactcg
     4381 cctcgcgcag cggtgcgaac accttcgagc aggtcctcgg gttcggtgtc cttgagcagg
     4441 tacccgatgg caccggcgtc gagcgcagcc acgatccgct cggtgtcgga gaacgacgtg
     4501 aggacgacca cctggacctg cttgtcgtcg gggctgtccc gaacgatgcg gcgggtggcc
     4561 tccacaccgt cgagctgtgg catctgcaag tccatcaaca cgacgtccgg ccgtttcgcc
     4621 tcgaccagcg tcacggcctc gacaccgtcg gcggcagtcc cgacgacctc gatgccgggg
     4681 tcggcatcga gcagccgctc gaggccacgg cggaccagcg cgtggtcgtc tgcgagcacc
     4741 actgtgatca cgagcgcctc acttccagtg tcacccgggt cccggcaccg ggagcggact
     4801 gcacgtcgag gatccccccg acatctcgga ccaggtcccg cagaccacgc aggccgagat
     4861 ggtcggtcgg cggtggcgcg gaggtgtcga agccgacacc gtcgtcgacg acctcgagct
     4921 tgatgcctcc ctcggggcgg gtgacgatca ccgcgagccg agtcggcctg ccatgacgct
     4981 gggcgttccg caccgcctcc tgccccactc gccagaccag cgcgatgacc tcgggcggta
     5041 cgtccgagtg gtcagccacc tgtacgtcga cggcgatgcc cgccgcgatg cacggcgaga
     5101 cgaggtcgtc gaacgccgcg gcgagaccct cggtctgtag ctccggcggg tagatctcca
     5161 ccaacagtga ccgcagcgaa cgcaggctgt gccggacgcc gccggcgatc tcctccagcc
     5221 gccgcgcccg ctccggttct ccccgcgagt cacgggctgc cgccgacagc tcgaacgacg
     5281 taccggccag ctgctgcacc acgccgtcgt gcaggtcgcg cgcgatgcga cgtcgctcgg
     5341 tgtcagaagc ccggaccgcc gccagcagca gccgctcacg gttcgccgca gccgcgtcga
     5401 ggcgccgggc gaggacgtac acgaggggag ccgtcatggc gaggaagatc agcagtccgg
     5461 ccaggatgat cgggcggaac gcggacagca cctgcgcgct gcggtcggac acgtcgtcgt
     5521 aggagaagta cgcctcgaag agcagcggct tgccgcccgg tgcccagatc tgtgagtaca
     5581 cctccaacag ccgtccgttg ccacgctcga acttgttctc ggccttgttg aggtcggaca
     5641 cctctgccgc cgtgccgccg tcgtcgatga tcgcgacttc cgcgggctcc agctcgaacg
     5701 tgtcgccgat gaggcgcggg tcgtcggagt acaggatcgt gccgtcgcgg tcccagatct
     5761 tgatgcggag gatgtcgccg cccatcacgc gcttgcgcat ctgccggtcg aacttgtcca
     5821 gtgctgcggc gttctggtcg acaacgcctc gggtgagcgt gggctcgacg gccgagcgcc
     5881 ccagcagctg tgtggtggcc cgggcatcgg tgatcgcctc gtcggtggcg gcacgctggc
     5941 tgagccagcc tgacaccgcg agcagcagca acaacgccac caaaccggca gcgaggaact
     6001 gtacgacggg gttggagacg aaccgtcgaa gcgtgtccac tgccgacgcc gcacgaggcg
     6061 gcgcgactgc cgacccgaac cgcgtcgacg cctgcggacc cggcgcaggc gaggtcgaca
     6121 cagggtcggt cgactggcgg gcaccaggca tcgagatccg tccgatccgt gcggtttcgg
     6181 gaacctcagt atgccggtac gcccgcgtca gaaccgcaac gagccgcgac aaacctggcc
     6241 actcgccggc tggcgcgcgc gccagccgat cgtgtccgta ccggccaggt tcagcatcag
     6301 ccggcgtacc tcgaatgagc cgctcggcgg tttggtcttc ctcgtgccat gtgcggacac
     6361 gtcgccgttg tggaggatgc gccacttcca gacctggccg ttgcggctgc tgtccacttc
     6421 agcctcgacc tcgatacggc cgtcctccgg gctggctttc aacttccact ccgcatgtcc
     6481 cgaacagctc ccgcgccgga tgacgtcgcg gtcgttggcg tcggcgggcg tcaggccgcc
     6541 cgcggcaatc gccatggcga cggcggcgac cggcacggca ggtgtccaca cccgtcggcg
     6601 gctgcggcgc agccttccac ggttgcgctc cacctcccag gtggtcttca tgtcgtcctc
     6661 cgtcgttcat gctgagtcgg tgcaggatga tccgacgaaa ggatcgctca ctcggcgggc
     6721 agttctccat tacccggtgg tactacgact cacagcttct tctgcatctc ggccaggcgt
     6781 tcgacaggcc ggaagccgag cgtctcgttg atcgccacca tcgggccgtt ctcctcggcg
     6841 ttccacgagt ggacgaccgt cgagtcgggg aacagcggtt ggaaccgccg gaggttctgc
     6901 gccttcatcg cgattccgag ccgatgaccg cgagcactct tcagcaccag cgtgccgcct
     6961 tgccaagcga cagctcggtt ctcctcggtg gtcatcagct gggtgagccc gacgagagtg
     7021 ccgtcggatg cgatcgcgac cgtcgacacc tcgtggcgtc cttgcttgcg gaaccgctcc
     7081 tctgtgctcc gcacgcgctc cgcgtcccag acctcgttct ccagctcgag ctcgcccatc
     7141 ggagcctcgc tgttgaacgc gatctgcagt tcacagaagc cgtccatgta ctcctcgggc
     7201 actgcgtcga tccatgtcga gagacggaaa gcatggtggt gaggcgccgc cgccgcggtc
     7261 agctcgtcca agcgacgatc ggacaccgga aggtcgagga cgcgatggat gtcgaggatg
     7321 ccgagctcga agccgtgtcg tcgagtgaac gtcaggccgg cggactcggc ctcaggatcc
     7381 agcggcaggt tgacttcggt caccgcggtc gtccggccgg ccacacgcgc catgtcgagc
     7441 gatgtctcga ggagcgcgct tccgacgccg cgcctccggt gctctggaag gaccatcggc
     7501 gcgacgaatg ccatgtgggt gttgtcggcc agcgggagca tcagcttgtc gacaccgacc
     7561 atcccggcac ggtcgaacgc ggcgatcggc tgctgcctga acgggctgtt ctccgctcgt
     7621 aaggcaacca tggctgcgcg cagcgaccag tacgccgcat agtcgcggcc atgagcgtcg
     7681 gcggatcgcg ccacctccca ccaccgggcc acctcatcgt cgtcatggac gtcgatctcg
     7741 cgtacctgca tgaccgaaaa gcgtaggagc agccgaggct tcggccaacg ggtttccacc
     7801 gactacagcg cgagcgaggc tttcacgacg tcggcgagcc tgtgggcgac ggcgtcggcc
     7861 tgctcggccg acggagcctc caccatcacg cgtaccagcg gctcggtccc ggacgggcgc
     7921 agcaggaccc ggccggtctc gccgagctcc gcttcggccg ctcgcacggc cgactgaacc
     7981 gtggcatcgg tcgtcacccg agcccggtcg acgtccggca cattcaccag cgtctgcggc
     8041 aggcgctgca ccagccctgc gaggtccgcg agcgatctcc gctgagacgc catccgctcg
     8101 gcgacgtgga ccgccgcgag tacgccgtcc ccagtggtcc catacgtgct catgatcacg
     8161 tgcccagact gctcgccgcc caggacgtat ccacccgagc gcatctcctc gaggacgtag
     8221 cggtcgccca cgttcgcggt gacgacgccg actcccgcct cgcgcatcgc gagcacgaac
     8281 cccaggttgc tcatcacggt ggcgaccacg gtgttgccgg gcaacgcgcc ggagtcacgc
     8341 agggccagcg ccaggatcgc gaggatctgg tcaccgtcga cgacctcgcc tgactggtcg
     8401 acggccaggc agcggtcggc atcaccgtcg aacgcgaagc cggcgtcggc accgtgctcg
     8461 agcacggccc gctggaggcc ggcgatgtgg gtcgacccgc atcgcacgtt gatgttgaac
     8521 ccgtccggct ggtcgtggat ggtgcggact tcggccccga gagcacggaa cacgtgcgga
     8581 gcggtggccg atgccgcccc ctcggcgcag tcgagcacga aactcagccc ggccagcgat
     8641 gaggtgaccg tcgacgccag gtggtcgacg taccgtccga ccgcatccgg atcgtcggtg
     8701 acacgtccca cctcggctcc ggtcggacga tcccacggct ggcccatcgc ctgctcgatg
     8761 gcgtcctcga cgccgtcctc gagcttgtgt ccaccacggg cgaagaactt gatcccgttg
     8821 tcgggcatcg ggttgtggga agccgacagc atgacaccga agtcggcgcc catctcggcg
     8881 gtcaggtagg ccacggccgg cgtcggcagg acgcccagcc ggcgtacgtc gacacctgcc
     8941 gacgccaagc cgccgaccac gacggcttcg aggaactccc cactcgcccg cgggtcacgc
     9001 ccgacaacgg caaccggccg tcgtctcgat ctgaatccgg actgaccgag ggttcgagcg
     9061 gccgccacgg acaggtcgag ggcgagctcg gcagtgagct cccggttggc gacgccccgg
     9121 acgccgtctg tgccgaacag gcgggatacc acggtgtgag cgtctgtctg cgtcggcgcc
     9181 gcaggtcagc gcttgctgta ctgcggagcc ttccgggcct tcttgagacc ggccttcttg
     9241 cgctccttga tccgggcgtc gcgcgtgagc atcccggcct tcttcagcgt ggagcggttg
     9301 gactcggtgt cggagtcgtt cagagcgcgc gagacaccca accgaagggc gccggcctgg
     9361 ccggtgaggc cgccgccgtg gatccgggcg atgacgtcgt agcggccgac gagctgcgtg
     9421 agcacgaaag gctcgttgac cagctgctgg tgcagcttgt tggggaagta cgcctccagc
     9481 ggacgaccgt tgacggtcca ggcgccggtg cccgggacga gtcggacgcg ggcgaccgcc
     9541 tccttgcgac ggccggtcgc accggccgcg gcgatcgtga cagcgcgagc agccacgtcg
     9601 gccgcgggag cactctcggt ggtgtactcg ctgggcgcgt ccgtgtccac cgggtcggcg
     9661 atgtcgacgg tctcgttggt ggtctcagcc acggatgttc ctcacttcaa agcctgcgtt
     9721 gcaagggttg agcggcacca gctcacgcgc tcactgcggg atctggacga tgtcgaacgg
     9781 cttgggctgc tgtgcggcgt gcgggtggtc aggtccgccg tacaccttca gcttcttgag
     9841 catctgacga ccgaggcggt tcttgggaag catcccccag acagccttct cgatcgcctt
     9901 gcgcggatcc ttgtcgagca gctcaccgta cgtcgtcgcg gtgagtccgc ccgggtatcc
     9961 ggaatgacgg taggccagct tggtcgcgcg cttgttgccc gacagcgcca ccttgtccgc
    10021 gttgacgatg atcacgaagt caccggtgtc gacgtgcggg gcgtacatcg tcttgtgctt
    10081 gccccgcagc agcgtcgcag cctggacggc gagccgtccg aggaccacgt cggtggcgtc
    10141 gatgacatgc cactcacgcg tgacgtcgcc gggcttcggg ctgtacgtgc gcacggtcgt
    10201 tgaccttcgt tctctacgga tatgaccaga tgtgcgtgat gcttcgggat gcgtcatctc
    10261 gccgccagac atgcagcgat gaacgtgttg ctcccgctga gcacaacgac cgcccaggat
    10321 accggggcgc acgccccacg gtcaaaacgg gccgcccggc gcgaaccggc gcgatccgcc
    10381 tggcgcaacc tcgttttcga aatcctcacc gggggcttag gttggtctca ccacgcacgt
    10441 gaaccccacg tgagctcaag tgacgcccac acctccaagg agagcggacg tgtcctcaga
    10501 ctctcacgaa gccgccacca gcgcggggaa cgcaaccgcc gaaagtggct cggcgggcgg
    10561 cgccacactc accgtccgcg acaaccggac cggagccgag tacgacctcc cgatcctcga
    10621 cggcacagtg cgcgcctccg acctggccaa gatccgcctc aaggacgacg agcccggcat
    10681 cgctgtctac gaccccggct tcgtcaacac ggcatcgtgc cgctcctcga tcacctacat
    10741 cgacggcgac aagggaatcc tcgagtaccg cgggtacccg atcgagcagt tggccgagaa
    10801 gtcgacctac ctcgaggtgg cctatctcct gatccacggc gagctgccca ccaagcagca
    10861 gttcgaggca tgggagcacc agatcacgtt ccacaccttc gtgcacgaga acgtgaagag
    10921 cttcatggag ggcttccgct acgacgctca cccgatgggc atgctgctcg cctcggtggg
    10981 cgcgctctcg acgttctatc cggagtcccg caacatcgcc gacccgaacg tccggcagat
    11041 gcagatcatc cggctcatcg cgaagatgcc caccttgggc gcctgggcgt tccggcatgc
    11101 gcagggcaag ccgtacatct accccgacaa cgaactcggg tacgccgcca acttcctcgc
    11161 gatgctgttc aggatgagcg aggagcagtt cgaggccgac gagcggctga tccgggcgct
    11221 cgaggtgttg ttcatcctgc acgccgatca cgagcagaac tgctcgacga acgccgtccg
    11281 ttcggtcggt tcttcgcagg tcgacccgta ctccgccgtc tccgccggca tcgcggcctt
    11341 gttcggacca ctgcacggtg gcgcgaacga ggcggtcctc aagatgctgc gccgggtcgg
    11401 ctcggtggac aacgtccctg agttcatcga cggtgtcaag aacggcaagg aacgcctgat
    11461 gggcttcggc caccgggtct acaagaacta cgacccgcgg gcgacgatca tcaagaaggc
    11521 gtgtgacgac gtcttcgagg tgaccggggt caacccgctg ctgaagatcg ccgtggagct
    11581 ggagaagatc gcgctggagg acgagtactt cgtcaagcgc aagctctacc cgaacgtcga
    11641 cttctactcc ggcctcatct acgaggcgct gagcttcccg ccggagatgt tcacggtgct
    11701 gttcgccatt ccgcggacgt cgggttggct cgcccagtgg ctggagctca ccgaggactc
    11761 cgagcagaag atcgcccggc ccaagcagat ctacaccggg caacgcaagc tggacttcgt
    11821 accgtcgtcc gaccgctggt cctagcgatc cgtttgccgg aggtctttcc ggcactcctt
    11881 ggattcttct ggcggacagg ttgcgctggg ttggtgccat cacccggtgt gctgccgtct
    11941 cgagctgagg tgggatggca gcgaagagcc ggggaacgtt cgtgcctgct gagctcgtca
    12001 gccgacgttc cgttccttgt gcctgatggg tgactgcctt gtacctgatg tgtgactggc
    12061 gggtttcgga catccgccgt cgccgagcgg cggtttgcat ggctacccag ccgaacccat
    12121 ccctcccaag gcagacctgc ctggctaccc gtccgttcgg atggctaccg aggcaaacca
    12181 ccaccgacat gtgcgattgg tggcctcggt ggctttccgg aggcgggtgg gccgcaccgg
    12241 taacaggcgc ggcccaaaag acctgagacc caccgtgtcc atcgaaggtt tgcgatgttt
    12301 acacgccttt tgggctgtaa acatcgcaaa ccgcgggccg cggcggatgg gtggcgccgt
    12361 aattgggtaa ctcgtccggg tgacctcgat ctggatggac cggccgggcc tggcgagcca
    12421 cccgccgctg accggtgacg cgacatacga cgtcgcagtc gtcggcggtg gcctgaccgg
    12481 cctcgtcacg gccgtgctgt tcgccagatc cggacggcgc gtggtgctcc tggaagctcg
    12541 ccggctcgcc gccgtcacca ccggcaacac caccggcaag atcagcctct tgcagggaac
    12601 gaagctttcg aggctggcga agcgacaccc ggctgatgtg gtccgcgcgt acgtcgacgc
    12661 aggccgggag ggtcaacagt ggctcctgcg ctactgcgac gaacacggcg ttgccgtgca
    12721 gacccgaccg gcgtacacct acgcgacgac cgcggaaggt gagcaggcgg cgcgcgagga
    12781 actcgagacg gctcgtcgtg ccggcctcga cgcggaatgg gtggagacga cagcgctgcc
    12841 gttcgacgta cgtggcgcgg tccggctggc cgaccaggca cagttcgacc cgatggacgt
    12901 cgtggcagca ctcgccgacg acctcgtcga acgcagcgga gtcatccacg aggagagccc
    12961 ggtgacgagc ctgcggagag gtgatggcgg tgtccgcatc gccgccccga ccgcaacgga
    13021 gcgtgccgag accgttgtcg tggcgacggg cactccgatc ttcgaccgcg gcggctactt
    13081 cgcccgtctc gaacccgatc ggtcctacgc agttgccttc cgatcgcctg ggacaccgcc
    13141 ggcgggcatg tatctctcgg ccgacacacc gacccgatcg ctgcgctcgt acccgcgcga
    13201 ggacggcgag tatctgctgg ccggtggcaa cggccacgtg gtggggcgcc gctcggcgac
    13261 cacccagcag tacgacgacc tggtccggtg gaccgagtcg atgtttccgg gagcacagcc
    13321 aactcaccgg tggtcggctc aggactaccg ctccgtggac ggactgccct acgttggacc
    13381 gctcacgccc ggacacgacg acgtcctggt ggcgaccggc tacgacaagt ggggaatgac
    13441 cacagccgtg gcggctgccc tcgcgctctc ggctcgcgtg ctcggaggtc agaccccgtg
    13501 ggcggacgcg atggacagct ggcggctgcg agagctcggc gggctgccga cagctcttcg
    13561 tctgaatgcg tcggttgcac tgcacctcgc ctccggctac ctgcaggcat tcggacacgg
    13621 cggttcggag atcggaccgg cggagggcga gggccgagtg ttccgccgcg cgggacgtcc
    13681 cacggctgcc tgccgcgtcg acggggtcac ccacgagctc tcggccgtat gtccacacct
    13741 tggcgggatc ctcacctgga acgacgcaga gcgctcatgg gactgtccgc tgcacggatc
    13801 acgatttgcc gccgacggca cccggctgga aggacccgcg acgaagggac tgccgccagc
    13861 ggtaccggct tgatgacggc gcgcatgcaa ggatcggagc atgcgttcgc ggcttcgtcg
    13921 cctggtcgac cggtcgatgg ggaagctgac gggcggtcga tcctcgcgtc aggtgcgacg
    13981 cacggcgtac gggtcgtccg gtgatctcgc acccggactg ttcgtcaacc cgatcgcaga
    14041 aggcgccgac ccggacgtgg tcaaggacgg tgaacgtttc ctgtggtgcc aggccgaggg
    14101 caacgtcggc atctcgatct ggacttccgc ccgcctcacc agtctgggca cgaagcacgt
    14161 ggtctggcgc gctgcggccg acggtccatg cagcaaggag gtgtgggcgc cggaactgat
    14221 caggctcgac ggccgatggc acgtgtactt cgcggcgtcg gacggtcgca accgcaacca
    14281 ccggacattc gttctcgtcg ccgacaccga cgacccgctg ggcagctaca ccttggaggg
    14341 tccgctcttc accggcgatc agcccggtgg tgacaacctc tggtcgatcg acttcacggt
    14401 cctcgagcac gacgagcggc ggttcgggct ctggtcgggc tggccggacg ccgacacgga
    14461 ccggcaggac ctctacatcg cggagatgtc gtcgccgacc gacgtgaagg gcggccgggt
    14521 ccgcatcgcc gaggccggcg tccatgactg ggaacgcatc tacgagtcgc cgaagagtcg
    14581 cggtctcctc gagggtccca gggtgttgca gcgcgacggg cgaaccttcg tcctgtactc
    14641 ctgcgccgcg tcgtggctgc cgacgtacaa gctcggcgtg ctcgagctga ccggcgacga
    14701 cccgatgagc cccagttcct gggcgcggtg gggccggcct gtcttcgcga gcaccgacga
    14761 gacgtacggg gtcgggcaca gttcgtgcgt ccacttcggc cagatcggca gcgcggacga
    14821 ggagtggtgg cacgtgttcc actccaaggt cgaccgcaga gacgggtggc gtcgcgccct
    14881 ccacgtacag cccatggagt ggcgccacga cggtacgccg atgctcggcg agccgttgcc
    14941 tcctgggcag gcgctcacca tgccgcgcgc ctatcgtgca cagcttcggc atgccggtgg
    15001 ctcgtgggac ttcggcgtcg acggtctcga cgacttcgac tactacggac accaccagta
    15061 cgtcgccgtc ggcccggaag gcctcgacct cggagtcgta cccgacacgc cggtgaacca
    15121 cttccgcagc ggggagaagg tcgtgctccg cggcggcgac ttcagcgacg tcgagatcga
    15181 agccacctgg acgttcgtgt cgtcgacgcg gtcggctggg gtgctcttcc gcaccaccgg
    15241 agcggccgtg gggtacgacg cccagcggtc gtacttcgcc gccatcgtcc ccaaggaaga
    15301 cacgctcgtg atcggaaaga cagacgggtc cacctggacg cgcatggcca ccacgaagat
    15361 gcccgtgcgg gtcgaccgtc cgcagacgct gcgcgtcagc gctgtcggta cgcacctcgt
    15421 cgtgacggcg ggtggaagca cgatcgaact ggacgacagc gaccacgccc gcggctccgt
    15481 cggcttgcga actgtgaaca cgcacgccag gttccggtcg ctgaccgtga gcccgttgcc
    15541 gacgtccgcg tcgtagggtc gcagacgtgg cagcagtgga cgcggtcgtc ttcgacctcg
    15601 gcaacgtctt gatcgagtgg gacccgcatc cggcgatcgc tgcaggagtt ggcgccgacg
    15661 aagccagccg attcctgact gcgaccgact tcgacttcgg ctcctggaac caccagcagg
    15721 actcgggcac accttgggtc gacgccgaac tcgccgtcga gaagagccac ccgcactggc
    15781 accggcacgc cacggcgtac cgccagcact tccaccggtc gctgctcggg gcgatccccg
    15841 ggaccgtcga catcctccgc gagctgcacg ccgccggcac agcggtgtac ggcctcacga
    15901 actggtccgc cgagctcttc cccgtggcgc gcgagagcta cgcgttcctc gacctcttcg
    15961 acgacatcgt cgtctccggc gaggaaggtg tcgcgaaacc ggacccgggg atcttcgacg
    16021 tcctccgaga acgtgtcgga cgaccgctcg acgcgtgcct gttcgtggac gacaacccgg
    16081 ccaacgtgga cgcggcgcgc acggccggta tggatgcgat cgtgttcacc ggccccgtcg
    16141 cgttgcgcgc cgagctggct tcccgcgacc tgctcggcgc catcgacggt tgagcctgtc
    16201 gagacccgga acccttgatc tcgacaagct cgatcagcgg acttccggcg gtcagcggac
    16261 ctccggcggt cagcggactt ccgcggtcaa cgggtgcgtt ggacgcggcc ttcgtcccag
    16321 acgggttggt cggactcgta gacagagccg tcggcgccga agaccatgaa ccggtcgaac
    16381 ccgcgggcga accagcggtc gtgggtgacc gcgacgaccg taccctcgaa gctctccagg
    16441 ccgtcctcga gcgcttcggc ggactccacg tcgaggttgt ccgtcggctc gtcaaggagg
    16501 agcagggtcg cacctgacag ctccagcagg aggatctgca tccgggcctg ctgcccaccg
    16561 gacaacgact cgaacctctg ctccgaggcg tgcgcgagct cataccgatc gagtgcccgc
    16621 gcggcctgtt cccgcggcat cccggcacgg tggtggtcgc cccggtgcag gatctcaagc
    16681 agcgtccggc cgatcagctc aggatgctcg tgcgtctgca cgaaccagcc gggacgcacc
    16741 ctggcaccca gccgcgcccg accggcgtgg tcgacaggtg ggatcggcac ctcaccgacc
    16801 gggcgatgct cgacatccgg gtcactgccc ccagccgcga gcagccgcag gaagtgcgac
    16861 ttgccggacc cgttggaccc gaggacggcg acccgttcgc cgtaccagac ctccaggtcg
    16921 aacggctgca tgaggcccgt gagctccagt gcctcacaga tgaccgcacg cttgccggtc
    16981 cggccgccgg tgaggcgcat cgtgacgttc tgctcgcggg ggatcgcttg cggaggtccc
    17041 ttctcctcga acttccggag ccgggtctgg gcggccttgt actgcgcggc catcccgtcg
    17101 ttgtactccg ccttgatctt gtaacggagt acgagcgcct tgagcttggc gtgctcctcg
    17161 tcccaccgcc ggcggagctc ttccagccgc gcgaatcgat cccgccgggc ctcgtggtac
    17221 gtcgcgaaac cacccgggtg gatccagacc gtgttgccgg cagccccgag ctcgaccgtc
    17281 acgatgcggg ttgcggtgtt gttcatcagc tcgcggtcgt ggctgacgaa caagatggtc
    17341 ttcggcgact cgcggatccg ttcctccagc cacagttttc ccggcacgtc gaggaagttg
    17401 tcgggctcgt cgagcaggag gacctcgtcc ggacctcgca gcaatgcctc gaggaccaac
    17461 cgcttctgct caccgcccga gagcgttgtc aagtcgcggt acttcgcctt gtcgtacgac
    17521 actccgagag cggcgatcgt acagacatcc cacagcacct cgctgtcgta gcctccggcg
    17581 tccgaccact cggcgagcgc ctcggcgtac gccatctggc tcttctcgtc gtcgtgctcc
    17641 atcatcgaga gctcggccgc ctcgacgtgt gcggccgcct ggcgtactcg cggcggggcg
    17701 accgacaaca gcaggtcgtg aacggtcagc ccgtcggtgc cctgggtgac cagctgccgc
    17761 atcacgccca gcccgccgga gcgtgtcacc gtccccgcgt gcggcgtcag ctcgccggtg
    17821 acgatgcgca gcaacgtcgt cttgcctgcg ccgttggcgc cgacgagcgc caccttggcg
    17881 ccttcaccca cccggaacgc cacgtcatcg aggaggacgc ggccgtcggg aagctcgtag
    17941 cgaacgccgg ccacgtcgac gtgtcccacg gccgttctcc ttcgtctctc agcgccccag
    18001 gtcagcggga cgctccatgg tcccccgacc gggccggccg cgcaccagat tttcctgccc
    18061 tgccgcggaa tccggcggtc gagcctgtcg agaccccgaa accccgagcc ctcgatctcg
    18121 acaagctcga tcagcggccc ttcacgagtc caggtcgttg acggccacct cgatcgcccg
    18181 atgaagcgtg gggtaggcgt agatcatcga acgcaaggat gccgtcggaa cctccccgtg
    18241 gatcgccacc acgagggctg acaagacctc gccgccgttc ggtccggcgc tggtggcgcc
    18301 gacgagcaga ccctggtcgg cgtcctcgac gagcttgatg aagccgtcgt tgccgacctt
    18361 gtggatccag ccgcgtgccg acgtcgtgag atccacatgt ccggtgcgca cgtcgatccc
    18421 ggcgtcgcgg
//
LOCUS       DATBFZ010000354         5585 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_2212793, whole genome shotgun sequence.
ACCESSION   DATBFZ010000354 DATBFZ010000000
VERSION     DATBFZ010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 5585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5585
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2212793"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            65..586
                     /locus_tag="VH419_10840"
     CDS             65..586
                     /locus_tag="VH419_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020578528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEY7044153.1"
                     /translation="MVPQSSPEPDRELGDPAELFVLFLDYYRKAITTKVDGLDEEQLR
                     SAVLPSGWTPLGLVKHLAFMERRWIVWGFLGRDVDEPWGDSDPDGPGWRVRDDETLAD
                     LLAAMRDGAVTTAEVARSHALTDSAAETGRFAGESRVPTLAWTLFHVLQEYARHAGHL
                     DVVRELIDGATGE"
     gene            complement(602..2335)
                     /locus_tag="VH419_10845"
     CDS             complement(602..2335)
                     /locus_tag="VH419_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012921077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY7044154.1"
                     /translation="MTDVDSDSTSRTATSAFREARDFLQAHREDYDTAYRGFDWPPLD
                     RFNYATDWIDVLAAEQGATTALWIVEEDETDTKLSFAEVVLLSQRLASYLDGHGVKRG
                     DRVLLLLGNVAPLWVAMLACIRLGAVLIPATTLLSAADLKDRIARGNVRAVIAAASDI
                     GKFTSDTGVDDLVKVSVGAAEGWLSYDEGVADDVEPLVGGDTAADDTLLLYFTSGTTA
                     QPKLVEHSHTSYPVGHLSTMYWIGLQPGDRHLNISSPGWAKHAWSCFFAPWNAGATVV
                     IYNYARFDPEKLLDVMERVGVSTFCAPPTVWRMLTQTPLGSRRLSLRECVGAGEPLNP
                     EVIEQIAKSWGITVRDGFGQTETTAQVGNSPGQPVKLGSMGRALPGYVVTLVDPVSGE
                     VRPDGEICLELARRPVGLMRAYRDDATRTDDAMRGGFYHTGDVASMDEDGYITYVGRA
                     DDVFKASDYRISPFELESVLIEHPAVAEAAVVPSPDPIRLAVPKAFVVLVAGEEPSRD
                     LAFDIFKFTREHLAPYKRIRRLEFAELPKTISGKIRRVELRADEAAEVASGSWPEHAY
                     SEDDFPDLKTT"
     gene            2444..5158
                     /locus_tag="VH419_10850"
     CDS             2444..5158
                     /locus_tag="VH419_10850"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020577602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY7044155.1"
                     /translation="MSDPAAEAHYPAHWEADVLLLDGGTARLRPIRPDDADLLVAFYD
                     RVSPESKYMRFFSPYPKLSDRDVWWFTNVDHTDRVAFVMTVGADMIAVGRFDKIEAGE
                     AEVAFLVEDKHQGRGVGQLLLEHLAQAGRERGFTKFIAEVLPENLRMIQTFRDFGYHI
                     EGGYEDGIMRLVFPIEATDTSVGVMRSREHRAEARSMQRLFEAQSVAIIGASRRQDTI
                     GSTLVRNLVLGGYRGRVYAVNPAADAVAGMPAYKTVQDIPDAVDLAVVAVPADSVSEV
                     VLDCAAKGVHGVVVVSSGFAEMGHEGRQRQRQLVSLARSYGLRLVGPNCLGVINTASD
                     VALNASLSPVMPPRGRVGFFCQSGALGVAILETVARRGLGLSTFVSAGNRADVSGNDL
                     LQYWKEDDATEVVLLYLESIGNPRKFSRIARRVGRDKPVVAVKSGRTTQGVPVGQPVP
                     STVAPPAAVDAMFRQAGVIQVETLDEMFDVALLLAHQPLPMGPRVAVVGNSDALGLLA
                     ADAAAASGLIVEQSIALSPEATAQDFEHALDAAIDNPAVDAVIAVFISVLTTSGEQVA
                     NVLAAVGEQSDKPLVCTFLGSQGVPELLRVPDLEGAAGRGSVPSYAAPEAAVRALARV
                     VTYGQWANRPRGTFPVFDDVRPGAARTMVSRLLAAEPDGVELDDAALGELLGCYGVSV
                     APLARVTTLAEAVAAAEGFGWNVVLKATADHLRQRPDLAHVWRNIDDVDDMTDAWENL
                     NDLIDEPDRADYVVQPMAEPGIPVCIGTLEDTLFGPIVSFGMAGTPSELLGDVAYRIP
                     PLTDGDAADLVRDVRAAPLFFGYRGSEAVDVAAIEELIHRVAQLKDDLPEVKRLELGL
                     VLAGADGAKVLRASGRLSPVVDARSDWYTRRMTVPMADDDTQVDGLFR"
     gene            5224..>5585
                     /locus_tag="VH419_10855"
     CDS             5224..>5585
                     /locus_tag="VH419_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5998 family protein"
                     /protein_id="HEY7044156.1"
                     /translation="MRESTLADRSDDLRAAIDRVGYYPAVVADAVNSALAGESVRSFV
                     IHHEPTFDHDEVRRHVTVLVLTPSRVIVAHTDEHPPDDLLPAPYASTSTEAVPLDQVG
                     SVAVTRMVANPAGKSEPSA"
BASE COUNT          842 a         1868 c         1965 g          910 t
ORIGIN      
        1 acatcgcaaa cctcaaccgc agcgtgtctg attggcggcc cgggcgagtc ggcatactgg
       61 cgcgatggtg ccacagtctt cccccgaacc ggacagggag ctcggcgacc cggccgagct
      121 cttcgtgctc ttcctggact actaccgcaa ggcgatcacg accaaggtcg acggtctgga
      181 cgaggagcag ctccggagtg ctgtgctccc gtccggctgg acgccgttgg ggctggtcaa
      241 gcatctggcg ttcatggaac ggcgctggat cgtttggggc ttcctcggtc gggatgtcga
      301 cgagccgtgg ggcgactcgg atccagacgg tccaggctgg cgggtccgcg acgacgagac
      361 gcttgccgat ctcctcgccg cgatgcgcga cggagctgtg acgaccgcag aggtggcccg
      421 cagccacgcg ttgacggaca gcgcggcaga gaccggccgg ttcgcaggcg agtcccgggt
      481 ccccacgctt gcgtggaccc tcttccacgt acttcaggag tacgcgcgtc atgccggcca
      541 cctggacgtg gtccgtgagc tcatcgacgg agccactggc gagtgacgcc ggcaggcgcg
      601 gtcaggtggt cttgaggtcg gggaagtcgt cttcggagta ggcgtgttcc ggccagctgc
      661 cggaggcgac ctcggcggcc tcgtcggcgc gaagctcgac ccgccggatc ttgccggaga
      721 tcgtcttcgg cagctcagcg aactccagcc ggcgaatccg cttgtacggc gcaaggtgtt
      781 cgcgagtgaa cttgaagatg tcgaacgcga ggtcgcggct cggctcctcg ccggccacca
      841 ggacgacgaa cgccttcggc accgccagcc ggatcgggtc cggtgacggc accaccgccg
      901 cctcggccac cgccggatgc tcgatgagga cgctctcgag ctcgaacggg ctgatgcggt
      961 agtcactggc cttgaagacg tcgtcggccc gtccgacata ggtgatgtag ccgtcctcgt
     1021 ccatcgacgc gacgtccccg gtgtggtaga agccgccccg catcgcatcg tcggtccggg
     1081 tcgcgtcgtc gcggtacgcc cgcatcaggc cgactggccg acgcgcgagc tccaggcaga
     1141 tttcaccgtc cggcctgacc tctccggaca ccgggtcgac cagggtgacg acgtaccccg
     1201 gaagggcccg tcccatcgac ccgagcttca ccggctgtcc gggtgagttg cccacctggg
     1261 ccgtggtctc ggtctggccg aaaccgtctc gcaccgtgat gccccacgac ttcgcgatct
     1321 gctcgatcac ctcggggttg agcggttcac cggcgccgac gcactcgcgc agcgacaacc
     1381 ggcgcgagcc gagtggcgtc tgggtgagca tccgccacac ggtcggcgga gcgcagaacg
     1441 tcgagacgcc gacccgctcc atcacgtcga gcagcttctc cgggtcgaac cgtgcgtagt
     1501 tgtagatcac gacggtagcg ccggcgttcc acggtgcgaa gaagcatgac caggcgtgct
     1561 tggcccaacc gggcgaggag atgttgaggt ggcggtcgcc cggctgcaac ccgatccagt
     1621 acatcgtgga gagatgcccg accgggtacg acgtgtggga atgctcgacc agtttgggct
     1681 gggcggtcgt gcccgacgtg aagtacagca gcagagtgtc gtcggcagcc gtgtccccgc
     1741 ccaccagtgg ctcgacgtcg tcggcgacgc cctcgtcgta cgacagccaa ccctcggcgg
     1801 caccgaccga caccttgacc aggtcgtcga cgcccgtgtc ggacgtgaac tttccgatgt
     1861 cggacgccgc ggcgatgacc gcacgaacgt tgccacgtgc gatgcggtcc ttcaggtcgg
     1921 ctgctgagag cagtgtggtg gccgggatca gcacggcacc gagtcggata cacgcgagca
     1981 tcgccaccca cagcggagcc acgttgccga gcagcagcag cactcggtca ccgcgcttga
     2041 ctccgtgtcc gtccaggtac gacgccagcc gctgggacag cagtaccacc tcggcgaagg
     2101 acagcttcgt gtcggtctcg tcctcttcga cgatccacaa cgccgtggtg gcgccctgtt
     2161 cggctgccag cacgtcgatc cagtcggtcg cgtagttgaa gcggtccagc ggaggccagt
     2221 cgaacccgcg gtatgccgtg tcgtagtcct cgcggtgggc ttggaggaag tctcgtgctt
     2281 cgcgaaaggc gctggtcgca gtacggctcg tgctgtcgct gtcgacgtca gtcatggggg
     2341 cagtctcgtc ggctttgccg gaagcgtaaa ggcaagccgg cagccgcgac agcggtggca
     2401 tcgacgacga ccgaccttgt cgaccacccg gtagattgcc ggtgtgtccg acccggctgc
     2461 ggaagcgcac tacccggctc actgggaggc tgacgtcctg ctgctcgacg gcggcaccgc
     2521 gaggctccgt ccgatccgcc ccgacgatgc cgacctgctc gtggcgttct acgaccgggt
     2581 ttcgccggag tcgaagtaca tgcgcttctt ctcgccgtac ccaaagctca gcgaccgcga
     2641 tgtctggtgg ttcaccaacg tcgaccacac cgaccgcgtc gcgttcgtca tgacggtcgg
     2701 ggcggacatg atcgccgtcg gcaggttcga caagatcgag gccggcgagg ccgaggtggc
     2761 gttcctggtg gaggacaagc accagggccg tggcgtcggt cagctcctgc tcgagcacct
     2821 cgcgcaggcc ggcagggaac gcgggttcac gaagttcatc gccgaagtgc tgcccgagaa
     2881 cctccggatg atccagacct tccgcgactt cgggtaccac atcgagggcg gctacgagga
     2941 cggcatcatg cggctggtgt tcccgatcga ggcgacggac acctcggtcg gcgtgatgcg
     3001 ctcgcgtgag caccgggccg aggctcggtc gatgcagcgg ttgttcgagg cgcagagcgt
     3061 ggcgatcatc ggtgccagcc ggcgccagga cacgatcggc agcacgctgg tccgcaacct
     3121 cgtgcttggt gggtaccgcg gccgcgtgta cgccgtcaac cccgccgccg acgcagtggc
     3181 gggcatgccg gcgtacaaga cggtccagga catccccgac gcggtcgatc tcgccgtggt
     3241 cgcggtaccg gcggactccg tctccgaggt ggtgctcgac tgcgcggcca agggcgtgca
     3301 cggggtcgtc gtcgtgtcca gcgggttcgc cgagatgggt cacgagggtc ggcagcggca
     3361 acgtcagctc gtcagcctgg cgcgttcgta cgggctgcgg ctcgtcggac ccaactgtct
     3421 gggggtgatc aacaccgcca gcgatgtcgc cctcaatgcg tcgttgtccc cggtgatgcc
     3481 gccgcgcggc agagtggggt tcttctgcca gtccggagcg ctgggcgtgg cgattctcga
     3541 gaccgtcgct cgccgcggcc tcggcctgtc gacgttcgtg tcagccggaa accgcgccga
     3601 cgtgtccggc aacgacctgc tccagtactg gaaggaggac gacgccaccg aggtggtcct
     3661 gctctacctc gagtcgatcg gcaacccgcg caagttctcg cggatcgcgc ggcgcgtcgg
     3721 tcgggacaag ccggtggtgg cggtgaagtc cggccgcacg acgcagggtg tccccgtcgg
     3781 acaaccggta ccctccaccg tcgcacctcc tgctgccgtc gacgcgatgt tcaggcaggc
     3841 cggcgtgatc caggtcgaga cgctcgacga gatgttcgac gtcgctttgc tgcttgccca
     3901 tcagccgctg ccgatggggc cgcgcgttgc cgtggtcggg aactccgacg cgctcggtct
     3961 gctcgcggcc gacgccgccg cggcatcggg cctgatcgtc gagcagtcca tcgcgctcag
     4021 cccggaggcg acggcgcagg acttcgagca cgctctggac gcggcgatcg acaatcccgc
     4081 ggtcgacgcc gtgatcgcgg tgttcatctc cgtgctcacc acgtccggcg aacaggtggc
     4141 caatgtgctg gcggccgtgg gggagcagtc cgacaagccg ctcgtgtgca cgttcctcgg
     4201 tagccaggga gtgccggagc tgttgcgcgt gcccgacctc gagggcgcag cggggcgtgg
     4261 ctcggtgccg tcctacgccg ccccggaggc tgcggtccgt gcgctcgcgc gcgtcgtgac
     4321 gtacggccag tgggccaacc ggccgcgagg aacgttcccg gtcttcgacg acgtccgacc
     4381 cggagccgcg cggacgatgg tgtcgcggct gctcgctgcc gaacccgacg gagtggagct
     4441 cgacgacgcc gcgctcggtg agctcctcgg ctgctacggg gtctcggtcg cgccgctggc
     4501 cagggtgacc acactggcgg aggcggtggc ggctgcggaa ggcttcggct ggaacgtcgt
     4561 gctgaaggcg acggccgacc atctgcgtca gcgccccgat ctggcgcacg tgtggcgcaa
     4621 catcgacgac gtcgacgaca tgacggacgc gtgggagaac ctcaacgacc tgatcgacga
     4681 gcccgaccgg gccgactacg tggtgcagcc gatggccgag ccagggatcc cggtgtgtat
     4741 cggcacgctg gaggacacgc tgttcgggcc gatcgtgtcg ttcgggatgg cgggtacgcc
     4801 gtccgagctt ctcggcgacg tggcgtaccg catcccgcca ctgaccgatg gcgacgccgc
     4861 cgacctggtc cgggacgtgc gtgccgcgcc actgttcttc gggtaccggg gatcggaggc
     4921 cgtggacgtg gcggcgatcg aagagctgat ccaccgggtc gcccagctca aggacgacct
     4981 gcccgaggtg aagcggctcg agctcggtct cgtccttgcc ggtgccgacg gcgcgaaggt
     5041 gctgcgtgcg tccggtcggc tcagtccggt cgtcgacgcg cgcagcgact ggtacacgcg
     5101 gcggatgacg gttccgatgg ctgacgacga cacccaggtc gacggcctgt tccgatgatc
     5161 gccggtccac agcctggtcc ggcgtgatgc ggcgggttcg gatcgtcgtg caaggatgac
     5221 gtcatgcgtg agtccaccct cgccgaccgt tctgacgacc tgcgtgcggc catcgaccgg
     5281 gtcggctact acccggcggt cgtggccgac gcggtcaact cggcactggc gggtgagtcg
     5341 gtccgcagct tcgtcatcca ccacgagccg acgttcgacc atgacgaggt gcggcgccac
     5401 gtcaccgtct tggtgctcac gccgagccgg gtgatcgtcg cccacaccga cgaacacccg
     5461 ccggacgacc tgttgcccgc gccgtacgcg tcgacgtcga ccgaggcggt gccgctcgac
     5521 caggtcggca gcgtcgcggt gacgcggatg gtggccaacc ctgccgggaa gtccgagccc
     5581 agcgc
//
LOCUS       DATBFZ010000355         1523 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_1554735, whole genome shotgun sequence.
ACCESSION   DATBFZ010000355 DATBFZ010000000
VERSION     DATBFZ010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 1523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1554735"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1096
                     /locus_tag="VH419_10860"
     CDS             <1..1096
                     /locus_tag="VH419_10860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044157.1"
                     /translation="TTPDHPTHLTSRRLGERMRIRKTISAALLAAVMLAGCSGTSGTW
                     VPHADGPRGPEASDKDNPADRTKNITDVLTELRDVDPCALLDREVIKTLTKVSVGPHL
                     PGRHLSECHVDTDIDEYTIGWSFGVDVGQLFHPAKQTQQEKINDEDYYTATDDTACSY
                     TRLVSPAVGVTLTTIAPLSNDGDDPCDMAKAYLTKAGATFTDLPRRDQQLTKPQIPLA
                     GHDPCETAPEVAKALHAETTSQPIRPYLCSIDPKPPAEDSPLAGQELTIAYEFKPDPR
                     DDLPNPGASPLPDGANLGDMTPVTIAGHRGTQFDGTGDLGCGIDLVVNEKTVLKDDTI
                     TYVQVMSTLAKDCGISKRAAEAVMARLDRT"
     gene            complement(1238..>1523)
                     /locus_tag="VH419_10865"
     CDS             complement(1238..>1523)
                     /locus_tag="VH419_10865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044158.1"
                     /translation="STIAEVRAGTHNKTILDFATQANSIVLKHMTLLEATGYVNDQTG
                     HFAEAAVRTTAYPENDLSATDIALGAVAFVVVSIGTVLAVRMLSARRASN"
BASE COUNT          318 a          571 c          429 g          205 t
ORIGIN      
        1 caccacaccc gaccacccga cacacctgac atcgcgacga ctgggggaaa gaatgcgaat
       61 ccggaaaacg atcagcgcag cactcctcgc ggcggtcatg ctcgccggct gctcaggcac
      121 gagcggcacg tgggtaccgc acgcagacgg cccccgcggt ccggaagcgt cagacaagga
      181 caacccagcc gaccgcacca aaaacatcac cgatgtgctc accgaactgc gcgacgtcga
      241 cccatgcgcg ctcctggacc gcgaggtcat caagaccctc accaaggtca gcgtgggccc
      301 gcatctaccc ggccgacacc tgtccgaatg ccacgtcgac accgacatcg acgagtacac
      361 gatcggctgg agcttcggcg tcgacgtcgg ccagctcttc catccggcca agcagaccca
      421 gcaagagaag atcaacgacg aggactacta caccgcaacc gacgacaccg cctgcagcta
      481 cacccggctc gtcagccccg ccgtcggtgt caccctgacg acgatcgcgc cgctttccaa
      541 cgacggcgac gatccgtgcg acatggccaa ggcctacctc accaaggccg gcgccacgtt
      601 caccgacctg ccccgccgcg accaacagct cacgaagcca cagatcccac tggccggcca
      661 cgacccgtgc gaaaccgcac ccgaggtcgc gaaggcactg cacgccgaga ccacgagcca
      721 gcccatccgg ccctacctct gctcgatcga tccgaagcca ccagccgagg actcaccctt
      781 ggcgggccag gaactcacca tcgcctacga gttcaaaccc gacccccgcg acgacctgcc
      841 aaacccgggc gcctcaccac tccccgacgg cgccaacctc ggcgacatga ccccagtcac
      901 tatcgccgga caccgcggca cgcagttcga cggcaccggc gacctcggct gcggcatcga
      961 cctcgtcgtc aacgaaaaga ccgtcctcaa ggacgacacc atcacctacg tgcaggtgat
     1021 gtcgaccctc gccaaagact gcggcatctc caaacgcgcc gccgaagcag tgatggcacg
     1081 actggaccgg acgtagcctc ggcgtacacg acgccgactt gacctcagat gaagcaccgg
     1141 ccaggtccaa cgatcgggca tctcatccgt gtgaatgcgt ggccagccgg aaagcgctgc
     1201 tccactgggc ttgcgacgcc gccgcagtgt tacaggatca gttcgaagct ctccgggcgc
     1261 tcagcatccg cacggccagc acggtgccga tcgagacgac gacgaacgcg accgcgccga
     1321 gcgcgatgtc cgtggcgctc agatcattct cgggatacgc ggtcgttcgt acggccgctt
     1381 cagcgaagtg cccggtctgg tcgttcacgt agccggtcgc ctcgagcagc gtcatgtgct
     1441 tcagcacgat gctgttcgcc tgggtggcga agtcgaggat cgtcttgttg tgggtgccgg
     1501 cccgcacctc ggcgatcgtg ctg
//
LOCUS       DATBFZ010000356         4044 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_2214691, whole genome shotgun sequence.
ACCESSION   DATBFZ010000356 DATBFZ010000000
VERSION     DATBFZ010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 4044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4044
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2214691"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..717
                     /locus_tag="VH419_10870"
     CDS             <1..717
                     /locus_tag="VH419_10870"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEY7044159.1"
                     /translation="LFLLVVAAAMIVALWELGRALAHNGTWLPALPMGVGAVGMIAGA
                     YYGGPDVLVVVLAGTVLASILWRMPRGQEGFVRDVTACVFCLVYVPFLASFVALLLSP
                     DDGVKRVLTFIFVTIASDIGGFAVGVLFGKHPMAPVISPKKSWEGFAGSAAACLLVGV
                     ACVVWLLDGDWWVGLVLGAVAVVAATLGDLAESLIKRDLGIKDMSNILPGHGGLMDRL
                     DSLLATVSVVWLVLNFLPVG"
     gene            799..1923
                     /locus_tag="VH419_10875"
     CDS             799..1923
                     /locus_tag="VH419_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012924026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEY7044160.1"
                     /translation="MSNRTGSFSRTRTLLAIGALVAAAATASATAATATTQSAGSYVA
                     RGHHQHPPAYAWHITPTGTAEEFRGLAAVSASTAWVSGEDGGVLRTVNAGETWQDVSP
                     PQAAGMALRDIEAFGPRRAVALAIGTGDASRIFATNDGGATWDEVFRNTDPNAFYDCM
                     AFSHDGLGLAMSDPVGGHFQLARTTDFGQTWAPFLPSSMPAALPTEFAFAASGTCIVS
                     SWGHTFWFATGGADTPRVFRSFDGGRHWTVHDTDMRGGPSAGIYSLAFRNPLQGIAVG
                     GDFQAETDGSDAAAWTLSFGQRWHNSTSPVGGYRSGVAFVPFTFNTAIAVGPTGSDVS
                     VDGGRSWTTFDTDRYDGVQCARDGACWASGTDGRVSRLVR"
     gene            complement(2077..2916)
                     /locus_tag="VH419_10880"
     CDS             complement(2077..2916)
                     /locus_tag="VH419_10880"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HEY7044161.1"
                     /translation="MGQLDVTSPANPRVKWLASLRRRRVRDDERATVVEGFAELELAL
                     QCGVRPHLVVVCPDLAGPDWATLIEPVRPLGSEVLTMSRPAFAKASYRESPDGWLAVV
                     ADPAGGLDRLDTVLERSATDGRAPLILVCEAVEKPGNLGAMLRTAEAAGVDAVVAASP
                     VADWGNPNVVRASKGTVFAVPIASAPAMKVAGWLREHGLCVVVATPEADVVVSDLDLT
                     GPTAVVVGAEHAGLSSLWYDADVVGPQVVAAALPMAGQVNSLNVATAAAIVLYEAVRQ
                     RRP"
     gene            2950..3567
                     /locus_tag="VH419_10885"
     CDS             2950..3567
                     /locus_tag="VH419_10885"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY7044162.1"
                     /translation="MLLHLVRHARPSIKPGRPASSWDLHEDAAIDIAALRSSGVLPDR
                     AAWFSSDEPKAVQTATQLGGSPRRLPGLREVERPADWLSSEEFVAVVTRSMQQPAVPG
                     RPGWESAAAVQARVYDTLLRHVFPVVQTQPAESSTDIVLVGHGTAWTMLVAALTDQPP
                     DVEAWRGMTTPDHCALELERGSVQDGTDRPARAKVVRRWGGWRST"
     gene            3601..>4044
                     /locus_tag="VH419_10890"
     CDS             3601..>4044
                     /locus_tag="VH419_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012922308.1"
                     /note="23S rRNA m2A2503 methyltransferase; methylates the
                     C2 position of the A2530 nucleotide in 23S rRNA; may be
                     involved in antibiotic resistance; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEY7044163.1"
                     /translation="MPEADPKQPETGATATLPLVFDEPRRAKKPPRHLADLTPAERRD
                     AVSKAGLPGYRARQVSHHYFTRLVSDPAEMTDLPADLRGPLAATLLPELLTPVHTLQA
                     DHGTTRKTLWRLHDGALVESVLMRYPGRVTMCVSSQAGCGMACPFC"
BASE COUNT          588 a         1412 c         1382 g          662 t
ORIGIN      
        1 ctgttcctcc tcgtcgtcgc tgcggcgatg atcgtcgccc tctgggagtt ggggcgagcg
       61 ctcgcacaca acggcacctg gctcccggct ctcccgatgg gagtgggggc ggtcggcatg
      121 atcgccggcg cgtactacgg cggaccggac gtcttggtcg tggtcttggc cgggacggtc
      181 ctcgcgtcga tcctgtggcg gatgccgcga ggccaggagg gttttgtccg ggacgtcacc
      241 gcctgcgtct tctgcctcgt ctacgtgccg ttcctggcgt cgttcgtcgc gctgctgctg
      301 tcgcccgacg acggggtgaa gcgggtgctc acgttcatct tcgtcacgat cgcgtcggac
      361 atcggtggct tcgccgtcgg tgtcctgttc gggaagcatc cgatggcgcc ggtcatcagt
      421 ccgaagaagt catgggaggg tttcgcgggc tcagccgcgg cctgccttct cgtcggagtc
      481 gcgtgcgtcg tctggttgct ggacggcgac tggtgggtgg gtctcgtgct cggggccgtg
      541 gccgtcgtgg cggctacgct cggagacctt gccgagtcgt tgatcaagcg cgacctcggg
      601 atcaaggaca tgagcaacat cctgcccggc cacggcggcc tcatggaccg gctggactct
      661 ttgctggcga ccgtctcggt cgtgtggctc gtgctgaact tcctgccggt cggctgaggg
      721 gtcaacgtcc agctgcgggt gcgcagcctg ccctgcctcg cgttgccgac gaccggagcc
      781 tcccctagcg tcgggaccat gtccaatcgc accggatcgt tcagtcgcac ccgaaccctt
      841 ctcgcaatcg gcgctcttgt cgctgccgct gccaccgcca gcgcgaccgc cgccaccgcc
      901 accacacagt cagcaggttc gtacgtcgcc cgcggtcatc accagcatcc accggcgtac
      961 gcctggcaca tcacgccgac cggcaccgcc gaggagtttc gcggccttgc tgccgtgagc
     1021 gcgagcaccg cgtgggtcag cggagaggac ggcggtgtcc tgcggacggt caacgccggt
     1081 gagacctggc aggacgtgtc accaccacaa gctgccggca tggcgctgcg cgacatcgag
     1141 gcgttcgggc ctcgtcgagc ggtcgcgttg gcgatcggta caggggacgc cagccggatc
     1201 ttcgcgacca acgacggcgg cgcgacctgg gacgaggtgt tccgcaacac cgacccgaac
     1261 gcgttctacg actgcatggc cttctctcac gacggtctcg gtctggcgat gagtgacccc
     1321 gtcggtggcc acttccagct cgctcggacg acggacttcg gccagacgtg ggcgccgttc
     1381 ctgccttcct cgatgccggc cgccctaccg acggagttcg cgttcgcagc gagcggcacc
     1441 tgcatcgtct ccagctgggg gcacacgttc tggttcgcga ccgggggagc ggacaccccg
     1501 cgcgtcttcc gcagcttcga cggcggacgc cactggacgg tccacgacac cgacatgcga
     1561 ggtggtccat cggccggcat ctactcgctc gccttccgga acccgttgca gggcatcgcg
     1621 gtcggcggtg acttccaggc cgagacggac ggctcggatg ccgctgcgtg gacgctgagc
     1681 ttcgggcagc gttggcacaa ctccacctcg ccggtgggtg ggtaccggtc aggtgtggca
     1741 ttcgtgccct tcacgttcaa cacggcgatc gcggtcggtc cgaccggaag tgacgtgagc
     1801 gtcgacggtg gccggtcgtg gaccaccttc gacaccgacc gctacgacgg ggtccagtgt
     1861 gcgcgggacg gggcgtgctg ggcgtccggg accgacggcc gggtctcgcg cctcgtccgc
     1921 tgacgggtgt ccggctagtc cggtggtcga gcctgtcgag acccgggcct ttgatctcga
     1981 cacgctcgat cagcgggatg ccgaggtggc gatcgacgtc cgacacgccg ccagaaagca
     2041 actaaacctg ccacctcggc gaagatcagg gatgggtcag gggcgacgtt ggcggacggc
     2101 ttcgtagagc acgatcgcgg cggcggtggc gacgttgagg ctgttgacct gacccgccat
     2161 gggcagtgct gcggcgacca cctgaggtcc gacgacgtcc gcgtcgtacc acagcgagct
     2221 gaggccggcg tgctccgcgc cgaccacgac ggccgtcgga ccggtcaggt cgaggtcgct
     2281 gacgaccacg tcggcctccg gcgtcgccac cacgacgcag agaccgtgct cacgcaacca
     2341 gcccgcaacc ttcatcgccg gcgccgaggc gatcgggacg gcgaacacgg tgcccttgct
     2401 ggcgcgtacg acgttggggt ttccccagtc ggctaccggc gaagcagcca cgacggcgtc
     2461 cactcccgcc gcctcggccg ttcgcagcat cgcaccgagg ttgcccggct tctcgaccgc
     2521 ttcgcagacc aggatgagag gagcccggcc gtcggttgct gagcgttcga ggacagtgtc
     2581 gagccggtcg aggccaccag cggggtcagc gacgacagcc agccagccgt ccggcgactc
     2641 gcggtacgac gccttcgcga acgccggccg gctcatcgtc agcacctccg aaccgagcgg
     2701 tcggacaggt tcgatgagcg tggcccagtc aggaccggcg aggtcgggac acacgacaac
     2761 caggtgcggc cggactccgc actgcagcgc cagctccagc tcggcgaacc cttcgacaac
     2821 cgtcgcacgt tcgtcatcgc gcaccctgcg tctccggagc gaggccagcc acttgacccg
     2881 cgggttcgcg gggctggtca cgtcgagttg gcccacggcc acgagcgtag tgatccgtag
     2941 ggttgggcca tgttgctgca cctggtccgg cacgcccggc cgtcgatcaa acctggtcga
     3001 ccggcctcgt cctgggactt gcacgaggac gccgccatcg acatcgcagc actacgatcc
     3061 agcggcgtac tgccggatcg cgccgcatgg ttcagttccg acgaacccaa ggctgtccag
     3121 acggcgaccc agctcggtgg atctcctcgc cggctgcctg gcctgcgtga ggtcgagcga
     3181 ccggccgact ggctgtcgtc agaggagttc gttgctgtcg tgactcggtc gatgcagcaa
     3241 cctgccgtgc cgggccggcc gggctgggag tcggccgccg cggttcaggc acgggtgtac
     3301 gacacgttgc tgcgccacgt attccctgtc gtacagaccc agcccgccga gtcttccacc
     3361 gacatcgtcc tggtcggtca cggcaccgcc tggaccatgc tggtcgcggc gttgaccgac
     3421 cagccgcccg acgtcgaggc atggagaggc atgaccacgc ccgaccactg cgccctcgag
     3481 ctcgagcgag gttcggtgca ggacggaacc gaccgaccgg cccgcgcgaa ggtcgtacga
     3541 cgctggggtg gctggcgctc gacctgagag gtctctccac cgtgagacac tgatccggcc
     3601 atgcccgaag ccgacccgaa gcagccggag accggcgcga ccgccacgct tccgctcgtc
     3661 ttcgacgagc ctcgacgggc caagaaacca ccgaggcacc tcgccgacct gacaccggcc
     3721 gaacgacgcg acgccgtcag caaggccggc ctgccgggtt accgggcgcg gcaggtgtcg
     3781 caccactact tcactcggct cgtcagcgac ccggccgaga tgacggacct tcccgccgac
     3841 ctgcgcgggc cgctggctgc gaccctcctc cccgagctcc tcacgccggt ccacacgctg
     3901 caggccgacc atggcacgac ccggaagacg ctgtggcggc tgcacgacgg ggcactcgtc
     3961 gagtcggtgc tgatgcgcta tcccggccgg gtgaccatgt gcgtgtcctc ccaggccggt
     4021 tgcggtatgg cgtgcccgtt ctgc
//
LOCUS       DATBFZ010000357         5023 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_1229940, whole genome shotgun sequence.
ACCESSION   DATBFZ010000357 DATBFZ010000000
VERSION     DATBFZ010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 5023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5023
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1229940"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..386)
                     /locus_tag="VH419_10895"
     CDS             complement(3..386)
                     /locus_tag="VH419_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002532933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEY7044164.1"
                     /translation="MATGKVKWYDADKGFGFLTTDDGGDVFVRSDALPAGVTTLKSGT
                     RVEFGLVQGRKGDQAIQVTVLDPTPSVSKAMRKKPEDMVNIVEDLMKLLENLEHTYRR
                     GRHPDSRAAKQTAQLLRALAAELEL"
     gene            complement(489..1388)
                     /gene="larE"
                     /locus_tag="VH419_10900"
     CDS             complement(489..1388)
                     /gene="larE"
                     /locus_tag="VH419_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013861469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HEY7044165.1"
                     /translation="MPDAVAAPFDPGLDGDPELTGRLAALDSRLTELGSVLVAFSGGA
                     DSAFLLTAATRVLGPERVVAATAMSDSLPAAELAAARHFAADIGVRHVTPKTREMDRA
                     GYRANAGDRCYFCKAELAEVLTPLAADLGLDWVATGTNADDAVAGFRPGIRAAAERGA
                     VTPLLDARLTKADIREVSREWGLSTWDKPAAACLASRIAYGIEVTPARLSRVERAEVS
                     LRGLLAAYDVDVTDLRVRDLGGRARIELDSAALARVSGDAVLDEASVGAVCEAGFDAG
                     FVDPTGFRSGAMNDLLTDPERYR"
     gene            1567..4557
                     /locus_tag="VH419_10905"
     CDS             1567..4557
                     /locus_tag="VH419_10905"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEY7044166.1"
                     /translation="MPRSSARRCLPGVRRLQPWIPLLAVAALVTAPTLYATGAAAAGT
                     TSPVAAREGADAAGTYLVILRSEPLATYDGDLRGYARTAPLAGHRFDADRPAVHAYER
                     RLLAEQAQVLSQLGSPPVLYHYTTALDGFAASLTGDQVAALRDHSDILTIERARIEHL
                     AGTPPGRAVDRATAARTTPLIAAKQAGSGTVVGVVDSGIWPENPSFAAVPLDLGTMRS
                     TYPGFTGSCETGERWSSSDCNAKVIAARYFLAGFGASHLSAAEFTSARDGSGHGSHVA
                     AVAAGNPGVDVSVASQDFGHVSGVAPAAGIAIYKACWAAPDPSNDGCSTADTAKAIDQ
                     AVRDGVDVLNYSVSGHDDTLTDVIEVAFLNAASAGVFVAAAAGDDGPGAGTVSHSGPW
                     VTSVAASQGNRYRGTVVLGNGRRISGAMVANRPVHETRMVYAGDVPVASASRRAASHC
                     LPGSLDARKVEDAIVVCDRGLTGRVTKSQTVSQAGGVGMVLVNVHLAGVDADLHAVPT
                     VHVSADDGAVVRRYIERARHPVGTLRPDRTGRTAPTVADFSARGPATVSGGDILKPDV
                     TAPGVSILAAVAPPSNFGHLWDLYSGTSMATPVVSGLAAVVAAGHPSWSPAMIKSALM
                     TTARPIGGGTPLDQGAGEVAPAAAFDPGLVYDAGRADWLGYLRGSGGPAARAADHTVP
                     VLDASDVNAASIAIGSLVGAQTVTRTVTNVGRAAETYTAHVDGLHGVAVSVSRPTLRL
                     PPGRSASFDVTFSARRTARYDRFAHGSLTWRGSSGHRVTSPIVVRPQLVEAPVELAGA
                     ATTGSLDLRARSGVTGTIRAVSSGLVGVSPIGYVLRTGSFDPASPQRSPSTKAHRFRI
                     QAGARFARFEVQTPGIGDDLDLYVYREGRLVGAATHESGDERITLVDPRPGAYTAYVH
                     AASASGGVTLGSLTGWVIRGPARPAAGNEVVAPLTVQPRPVTVTGGRPFDLQVGWRQL
                     DHTRRWLGVVTYDDSTRYTFVTVN"
     gene            complement(4565..>5023)
                     /locus_tag="VH419_10910"
     CDS             complement(4565..>5023)
                     /locus_tag="VH419_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY7044167.1"
                     /translation="QQDDKTAEAIDQDGWMHTGDLAVMRDDGYANIVGRIKDLVIRGG
                     ENVYPREVEEFLYTHPDIADVQVIGVPDERYGEEICAWIRMREGAQPLDADAVRGFCS
                     GRLAHFKIPRYVMVVDEFPMTVTGKVRKVEMREKSVAELGLQAAQSIRNA"
BASE COUNT          706 a         1841 c         1709 g          767 t
ORIGIN      
        1 tcctagagct cgagctcggc cgccagggca cgcagcagct gcgcggtctg cttggcggcc
       61 cggctgtccg ggtggcggcc ccggcggtag gtgtgctcga ggttctcgag aagcttcatc
      121 aggtcctcga cgatgttgac catgtcctcg ggcttcttgc gcatcgcctt cgacaccgac
      181 ggggtcgggt cgaggaccgt gacctggatg gcctggtcgc ccttgcggcc ttggacgagc
      241 ccgaactcca cacgggtgcc ggacttcagc gtggtgaccc cggccgggag ggcatcggag
      301 cgcacgaaga catcgccgcc gtcgtcggtg gtcaggaagc cgaatccctt gtcggcgtcg
      361 taccacttga ccttgcctgt cgccacgtct tgaccaatcg ttctctcgcc gggcgcttct
      421 cgcgaccgct tctcgccgaa cagcaacaca cccccgagct gtcggaggcg cgcctccagc
      481 ctactccctc agcggtaccg ctcgggatcg gtgagcaggt cgttcatcgc acccgaccgg
      541 aagcctgtcg ggtcgacgaa accggcgtcg aaaccggcct cgcacacggc cccgaccgag
      601 gcctcatcga ggacggcgtc ccccgacacg cgggccagcg cagcgctgtc gagctcgatg
      661 cgtgcccggc cgccgaggtc cctgacccgc aggtccgtga cgtcgacgtc gtacgccgcc
      721 aacagtccac gcaacgacac ctcggcgcgc tcgacacgcg acaggcgggc tggggtcacc
      781 tcgatgccgt aggcgatccg agacgccagg caggcggccg ccggcttgtc ccacgtggac
      841 agtccccact cccgggagac ctcccggatg tcggccttcg tcagccgcgc gtcgagcagc
      901 ggggtcaccg caccgcgttc ggcagccgcg cggatgcccg gccggaatcc cgcgacggcg
      961 tcgtcggcgt tggtccctgt cgcaacccag tcgagaccga gatcggcggc cagcggtgtg
     1021 aggacctccg ccagctcggc cttgcagaaa tagcaccggt cgcccgcatt cgcgcggtag
     1081 cccgcccggt ccatctcgcg ggtcttcggc gtgacgtggc ggacgccgat gtcggcagcg
     1141 aagtggcgtg ctgcagccag ctcggcagcc ggcagcgagt ccgacatcgc ggtggccgcg
     1201 acgacacgtt cgggccccag gacacgggtg gcggcggtca agaggaacgc ggagtcggcc
     1261 ccgccgctga acgcaaccag gacgctgccg agctccgtca gcctggaatc cagcgccgcc
     1321 agccggccgg tgagctccgg gtccccgtcc aacccaggat cgaaaggcgc agcgaccgcg
     1381 tccggcacgg attagactct atccgaggca ttcggcccga ttcccccgcg gctgctctcc
     1441 tgtcgacgca tcgagtcgtc tccgaccgcc tccgctcgtc gagtgcacct ggccgcggcg
     1501 cggggggttg actgggcgag ccggcctgac cagcgtggac gtttgagttt cacggaggtg
     1561 gcacctgtgc ctcgatcctc ggctcgccgg tgcctgcctg gcgtccgccg tctgcagcct
     1621 tggatcccgc tgcttgccgt cgccgccttg gtcacggcgc ccacgctgta cgccacggga
     1681 gcggctgcag ccggcacgac gagccctgtc gccgcgcgcg aaggcgctga cgcggccggg
     1741 acctacctgg tgatcctgcg gtccgagccg ctggcgacgt acgacggcga cctccgcggc
     1801 tacgctcgca ccgccccgct ggccggccac cgattcgacg cagaccgccc ggccgtccat
     1861 gcctacgaac gtcgactgct ggccgagcag gctcaggtgt tgagccagct cggatcgccg
     1921 ccggtgctgt accactacac gaccgcgctg gacgggttcg ccgccagcct gacgggcgac
     1981 caggtggcag cccttcgtga ccactccgac atcctgacga tcgagcgtgc ccgcatcgag
     2041 caccttgcgg ggacaccgcc tgggcgcgcc gtcgaccgag ccaccgcggc gcgtacgact
     2101 ccgctcatcg ctgccaagca ggcaggcagc gggacggtgg tcggtgtcgt cgactccggg
     2161 atctggccgg agaacccctc gttcgccgcc gtcccgctgg acctcggcac gatgcggtcg
     2221 acctatccgg gcttcaccgg cagctgcgag acaggtgagc gttggagcag ctcggactgc
     2281 aacgcgaagg tcatcgccgc ccggtacttc ctggccggct tcggcgcctc gcacctgtcc
     2341 gccgcggagt tcacctcggc gcgcgacggt agtgggcacg ggtcacacgt ggcggccgtc
     2401 gcagccggca acccgggagt cgacgtgtcg gtcgccagtc aggacttcgg acacgtgagt
     2461 ggcgttgccc cggcggccgg catcgcgatc tacaaggcct gctgggcggc gcccgacccg
     2521 tcgaacgacg gctgctcgac cgccgacacc gcgaaggcga tcgaccaggc ggtccgggac
     2581 ggggtcgacg tgctcaacta ctccgtgagt gggcacgacg acaccctcac cgacgtcatc
     2641 gaggtcgcct tcctcaatgc cgcgtcggca ggcgtgttcg ttgccgcggc cgccggcgac
     2701 gatggtccgg gcgccgggac cgtgtcacac agcggtcctt gggtcaccag cgtggccgcc
     2761 agccagggca accgataccg aggcacggtc gtcctcggca acggacgtcg gatctccgga
     2821 gcgatggtcg cgaacaggcc agtgcacgag acgcgcatgg tgtacgccgg agacgtccca
     2881 gtggcgtctg cctcgcgccg tgccgcctcg cactgcctcc ccggttcact cgacgcacgc
     2941 aaggtagaag atgcgatcgt cgtgtgcgac cgcggcctga caggccgcgt cacgaagagc
     3001 cagacggtga gccaggccgg cggagtcggg atggtcctgg tgaacgtcca tctcgcaggt
     3061 gtggacgccg acctgcacgc cgtgccgacc gtgcacgtca gcgcggacga cggcgcggtc
     3121 gtccgccgct acatcgagcg ggcacgccac ccggtcggca cgctacggcc cgaccgcacg
     3181 ggtcgcacgg caccgacggt cgccgacttc tccgcgcgcg gaccggccac cgtcagcggt
     3241 ggcgacattc tcaagcccga cgtcacggcc cccggggtca gcatcctcgc cgcggtggcg
     3301 ccaccgtcca acttcggcca tctgtgggac ctgtactccg gtacgtcgat ggccaccccc
     3361 gtggtctccg gtctggccgc tgtcgtcgcg gccgggcatc cgagctggtc accagcgatg
     3421 atcaagtcgg ccctgatgac gaccgcgagg cccatcggtg gcggcacacc gctggaccag
     3481 ggcgccggcg aggtcgcgcc ggcggcggcc ttcgaccccg gtctcgtgta tgacgccggt
     3541 cgcgccgact ggctcggcta cctgcgtggc agcggtggcc cggcggcgcg agctgccgac
     3601 cacaccgtgc cggtcctgga cgcctctgac gtcaacgcgg cgtcgatcgc gatcggatcc
     3661 ctggtcggtg cacagaccgt cacccgtacg gtgaccaacg tcggtcgagc ggccgagacc
     3721 tacaccgcgc acgtcgacgg cctgcacggc gtcgccgtct ctgtgtcgcg gccgacgctg
     3781 cggcttcccc ccggtcggtc cgcgtccttc gacgtcacct tcagcgcccg ccgcaccgcc
     3841 cgctacgacc ggttcgctca cggctcactc acatggcgtg gctcgagcgg ccaccgcgtg
     3901 accagtccga tcgtcgtacg tccgcagctc gtcgaggcac ccgtcgagct cgccggtgcg
     3961 gcgaccaccg gatccctcga tctgcgtgct cgatccggtg tcacggggac gatccgggca
     4021 gtgtccagcg gactcgtcgg tgtgtcgccg atcgggtacg tgctgaggac cggctcgttc
     4081 gaccccgcgt cgccgcagcg cagtccgagc accaaggcgc atcggttccg tatccaggcc
     4141 ggggcgaggt tcgcgcgctt cgaggtgcag acgccgggca tcggggacga tctcgacctg
     4201 tacgtctatc gcgaggggcg gctggtgggc gcggccaccc atgagagcgg tgacgagcgc
     4261 atcacgctcg tcgatccgag gcctggtgcg tacaccgcgt acgtccacgc cgcctccgcg
     4321 tccggcggcg tgacgctcgg ctcgctcacc ggctgggtga tccggggccc ggctcgaccg
     4381 gccgcaggca acgaggtcgt cgcgccgctg acggtgcagc cgcggccggt cacggtgacc
     4441 gggggccgac ccttcgacct ccaggtcggc tggcggcagc tcgaccacac caggcgatgg
     4501 ctcggtgtgg tgacgtacga cgactcgacg cgctacacgt tcgtcacggt gaactgactc
     4561 gacgtcaggc gttcctgatc gactgggccg cctggagacc gagctcggcg acggacttct
     4621 cccgcatctc gaccttgcgc accttgccgg tgacggtcat cgggaactcg tcgacgacca
     4681 tgacgtagcg cgggatcttg aagtgcgcga gcctgccgct gcagaatccc cgcacggcgt
     4741 cggcgtcgag cggctgcgca ccctcacgca tccggatcca cgcgcagatc tcctcgccgt
     4801 acctctcgtc cgggaccccg atgacctgca cgtcggcgat gtcggggtgg gtgtagagga
     4861 actcctcgac ctcgcgcggg tacacgttct cgccgccacg gatgaccagg tccttgatcc
     4921 ggcccacgat gttggcgtac ccgtcgtccc gcatcaccgc gagatcgccg gtgtgcatcc
     4981 agccgtcctg gtcgatcgcc tccgccgtct tgtcgtcctg ctg
//
LOCUS       DATBFZ010000358         1729 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_2216442, whole genome shotgun sequence.
ACCESSION   DATBFZ010000358 DATBFZ010000000
VERSION     DATBFZ010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2216442"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(164..382)
                     /locus_tag="VH419_10915"
     CDS             complement(164..382)
                     /locus_tag="VH419_10915"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HEY7044168.1"
                     /translation="MRRIQLHLDEELDDALAREAARRGQPKALVIREYLWREVDRRGV
                     VKDSLDELIGLSGTKPREGESIDDVVYG"
     gene            complement(400..1113)
                     /locus_tag="VH419_10920"
     CDS             complement(400..1113)
                     /locus_tag="VH419_10920"
                     /inference="COORDINATES: protein motif:HMM:NF013570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HEY7044169.1"
                     /translation="MTTDLLFLRDAYLRSARAEVTHVDGVRIALDRTVFYPTGGGQPH
                     DTGTLSWDGGTAAVTEVRKGGDAVWHTLEGDIPAPGTEVTAEIDWDRRHRLMRTHTAL
                     HVLCGVIWHEWGVPVTGGNMEPLSARMDFELDPTPEGFGARVEELVAAELAANRPIEV
                     SFVPRSEAVLDEALIRTKVSLVPETVAEIRVVDIVGLDRQADGGTHVRSTAEVGQVRV
                     TKTESKGKGNKRVRIEVTD"
     gene            1239..>1729
                     /locus_tag="VH419_10925"
     CDS             1239..>1729
                     /locus_tag="VH419_10925"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY7044170.1"
                     /translation="MRQQSRGLRLIAVFLGLSLLAAACGDDDDTESSGTTEDTSDQSA
                     APATGEAAPSCTGEENGTLTIGALLPETGDLAGLGPPEIAGAQLAVKEINDAGGVNGA
                     PIQYLPGDSGDSDPDIANPTVDGHLEAGADMILGAAASGISLNVIDKITGACVIQFSP
                     ANTS"
BASE COUNT          266 a          596 c          578 g          289 t
ORIGIN      
        1 tcgtggccgt cacgtcggtt caggaacgtc caggtctctc cgaggatgag gttggtcgtg
       61 aggatcggtt cccggttcgc ctgctggatc gcgacggccg cctcgtggtg gcgttcgcgc
      121 cttcgcacca gcgcgatcca gaagctggtg tcggcgaacc tcgtcatccg tagacgacgt
      181 cgtcgatcga ctcgccctcc ctgggcttcg tacccgacag gccgatcagc tcgtccaagg
      241 agtccttcac gactccgcgc cggtcgacct cgcgccagag gtactcccgg atcaccagcg
      301 ctttgggctg gcctcgcctc gcggcttccc gcgccaaggc gtcgtcgagc tcttcgtcca
      361 ggtgaagctg tatacgcctc atggcaggag tatacacact cagtcggtga cctcgatgcg
      421 gacccgcttg ttgcccttgc ccttggactc ggtcttggtc actcggacct ggccgacctc
      481 ggcggtggag cggacgtggg tgccgccgtc ggcctggcgg tccaacccga cgatgtcgac
      541 cacccggatc tcggccacgg tctcgggcac caggctgacc ttggtgcgga tcagggcctc
      601 gtcgagcacg gcctcggagc ggggcacgaa gctgacctcg atgggccggt tcgccgccag
      661 ctcggccgcg accagctcct ccacccgggc cccgaagccc tcgggcgtcg gatcgagctc
      721 gaagtccatc cgggccgaca gcggctccat gttgccgccg gtgaccggga cgccccactc
      781 gtgccagatc acgccgcaca ggacgtgcag cgcggtgtgg gtgcgcatca gccggtggcg
      841 ccggtcccag tcgatctctg cggtcacctc ggtcccgggt gcggggatgt cgccctccag
      901 cgtgtgccac actgcgtccc cgcctttgcg gacctcggtc acggccgcgg tgccaccgtc
      961 ccaggacagg gtgccggtgt cgtgcggctg gccaccgcct gtcggataga aaacggtgcg
     1021 atcgagcgcg atgcgcacgc cgtcgacgtg cgtcacttcg gcgcgcgcgg agcgcaggta
     1081 cgcatcgcgc agaaacagca aatcggtagt catcgtgatc gttccttaac cgaactgttc
     1141 gccagtttgc aacgcgggcc gtcccatcgg tagtagacct gtctcgccgg gaggggctac
     1201 ccttccgcgc tcaattgttc cggacaaggg gaagtgcgat gcgacagcaa agccgtggtc
     1261 tgcggctcat cgccgttttc ctcgggttgt cactgcttgc cgccgcatgc ggcgacgacg
     1321 acgacaccga gtcgagcggt acgacagagg acacgtccga tcagagcgca gcgccggcaa
     1381 cgggtgaagc ggcaccgagt tgcacgggtg aggagaacgg caccctcacc atcggcgctc
     1441 tgctgcccga gacgggcgac ctcgcggggc tcgggccgcc cgagatcgca ggtgcgcaac
     1501 tggccgtgaa ggagatcaac gacgcgggtg gcgtgaacgg cgccccgatc cagtacctgc
     1561 ccggcgactc cggcgactcc gacccggaca tcgccaaccc gacggtcgac ggccacctcg
     1621 aggccggggc agacatgatc ctgggtgcgg cagcgtcggg catctcgctg aacgtgatcg
     1681 acaagatcac gggcgcgtgc gtgatccagt tctcgccggc gaacacctc
//
LOCUS       DATBFZ010000359         4077 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_6033922, whole genome shotgun sequence.
ACCESSION   DATBFZ010000359 DATBFZ010000000
VERSION     DATBFZ010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 4077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4077
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6033922"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            68..895
                     /locus_tag="VH419_10930"
     CDS             68..895
                     /locus_tag="VH419_10930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044171.1"
                     /translation="MNEFESTLASALQAEADRTTLHLDTADAANLLEAGLDRIDRDRR
                     HRTWAAVLAAAAAVLVAGAVYINTDSPTSVQPAGTPSTTSTSPSAGTPNYQMPVSYAK
                     GVLLQAGVTLDLTLDGAWRIDNYPWLHDAGKYGGLAVYRPSALAAGTGCLTDRPNTNV
                     GRTPQLLAQQLAQLPRSTLLQPPTPTQKLGRQAIHLQLRINPNCGTGVYRVAQTLLGG
                     HGISYGRASAHVVIDFWVENVRGVPLVIETWHQDGASSHMVNEIARAVNTLTFTNGG"
     gene            complement(980..1300)
                     /locus_tag="VH419_10935"
     CDS             complement(980..1300)
                     /locus_tag="VH419_10935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044172.1"
                     /translation="MARDQAAPALRAVTILWYTVAFNLAALLISVVGIGFDSGAAWPL
                     WLPLASVSLCAMSGVFCLFGTQTRSFGAGCLGGTAVSILVFLVLFMIFFVTYFIVPGG
                     HDLS"
     gene            complement(1431..2153)
                     /locus_tag="VH419_10940"
     CDS             complement(1431..2153)
                     /locus_tag="VH419_10940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044173.1"
                     /translation="MDDVDFLARVRDLLAEQLVMDDVVVTAVRPAGGRPWPRTEATFE
                     LVDLPSSWQRPTAGSAYLPLGEEWRYARGCEEPSDYALLLAREIESAVVRLIKPTPPV
                     AALTPSVVTERWQWLLERLALHGPVRQLAPDRLVVTEDTASFTVFVTAEEWASIATPI
                     ELDADDPQDFGWHDPDENYLVFYEHDLVWSIRPELPPVRSGAELRRKIRAAQAEGRTN
                     VGWFAHPPTTPESAHDHDVDAG"
     gene            complement(2451..2909)
                     /locus_tag="VH419_10945"
     CDS             complement(2451..2909)
                     /locus_tag="VH419_10945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044174.1"
                     /translation="MRKLRLTPRLLAGALAFAAVVLGVFYWGNSAPAGSAFTYTAEQR
                     SRYLAEMKENLNGHPESPRYDLVSEQVLVSEGVVACEWLATQPSGDSALTNDELRHLY
                     FQEHPQATAGWPFGHGHTGLREEVLTNSWGWLCEDVEQDHRADPEPVEGD"
     gene            complement(3141..3323)
                     /locus_tag="VH419_10950"
     CDS             complement(3141..3323)
                     /locus_tag="VH419_10950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044175.1"
                     /translation="MNEALPPLLFLAVFGVLSLLDYEWAWLVFGVAFVAFAVVFYLLP
                     WTVNIARNFVAGYRGT"
     gene            3696..4076
                     /locus_tag="VH419_10955"
     CDS             3696..4076
                     /locus_tag="VH419_10955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044176.1"
                     /translation="MDRSGRQRSTSSRAHAADPTNRTIGAVAVLLGLACLTACSDRPE
                     SFQTACDHLAAANDAVAHGDITRAKVELNDAYDWGEPVYEDAAGTEYAAAVGDFYRAV
                     GMAVSHFGTEDGQQALNDAEHACS"
BASE COUNT          752 a         1368 c         1314 g          643 t
ORIGIN      
        1 ctggctcggt caagcagcac gcacaccggg ccaccgccac cctccaggag cggatggagt
       61 catggtcatg aacgaattcg agtcgacgct tgcctcggcc ctgcaggccg aagcagaccg
      121 gacgaccctg catctagaca ccgcggacgc ggccaacctg ctcgaagccg gattggaccg
      181 gatcgaccgc gaccggcggc accgcacctg ggccgccgtc ctcgctgccg ccgcagccgt
      241 cctggtcgcc ggagccgtct acatcaatac cgattccccg acgtcagtcc agccagccgg
      301 gactccctcc accacgagca cgtcgccgtc cgcggggacc cccaactacc agatgcccgt
      361 cagctacgcg aagggcgtcc tgctccaggc cggggtcacc ctcgacctca ccctggacgg
      421 agcctggcgg atcgacaact atccgtggtt gcacgacgcg gggaaatacg gcggtctggc
      481 cgtctaccgg cccagtgcgc tggctgccgg gaccggctgc ctcaccgacc ggccgaacac
      541 gaacgtcggc cgcactccgc agctgctcgc gcagcagctc gcccagctcc cgcgaagcac
      601 cctcctccag ccaccaacac cgactcagaa gttgggccgc caggccatcc acctgcagct
      661 ccggatcaac ccgaactgcg gcacaggcgt gtaccgcgtc gcccaaaccc tcctcggcgg
      721 tcacggcatc agctacggcc gcgcctccgc acacgtcgtc atcgacttct gggtcgagaa
      781 cgtgcgcgga gtcccgctcg tgatcgagac gtggcaccag gacggcgcat cgagccacat
      841 ggtgaacgag atcgcccgcg ccgtgaacac actcaccttc acgaacggcg ggtagggccg
      901 atctgtcgga ccacccacgg atcgatcagc gggctgacac cggcgaaagt gcgcacgtcg
      961 gggcatgacc ggatcgttct caggacaggt cgtgaccgcc cggaacgatg aagtaggtga
     1021 cgaagaagat catgaacagc accaggaaca cgaggatcga caccgcggtg ccgcccaagc
     1081 atccggcacc gaacgaacgt gtctgcgtcc cgaagagaca gaagacaccg gacatggcgc
     1141 acagcgagac ggaggcgaga gggagccaaa gaggccaggc ggctccggag tcgaatccga
     1201 tcccgacgac tgagatcagc aacgccgcga ggttgaaggc gaccgtgtac cagaggatcg
     1261 tcactgcacg gagtgctggt gccgcttgat cacgagccac gcgcagaccg taagggatcg
     1321 tcgctacgac tgtcctcgca gcggcagaac cgcgcaccat cggtggcaga tctgggtgat
     1381 gggtcggggc ggcctcgagg cgctcggtct ggcacgcgtc ggttgcccgt tcagccggcg
     1441 tcgacgtcgt ggtcgtgcgc ggactccggg gtggtcggtg ggtgggcgaa ccagccgacg
     1501 ttggttcgtc cctcggcttg ggcggctcgg atcttacgtc ttagttctgc accggagcgg
     1561 acgggcggta gttccggtcg gatcgaccag acgaggtcgt gctcgtagaa caccaggtag
     1621 ttctcgtccg ggtcgtgcca gccgaagtcc tgggggtcat cggcgtccag ctcgatggga
     1681 gtagcgatcg aggcccattc ctcggccgtg acgaagacgg tgaaggacgc tgtgtcctct
     1741 gtgacgacca accggtcggg agctagttgc cgcactggcc cgtggagcgc gaggcgctcg
     1801 aggagccact gccaccgctc ggtgaccact gacggcgtga gagccgccac tggaggggtt
     1861 ggcttgatca gccggacgac ggccgactcg atctcgcgag cgagtagcaa cgcgtagtcc
     1921 gacggttcct cgcatcctcg ggcatagcgc cactcttctc ccaggggcag atatgccgag
     1981 ccggcggtcg gccgctgcca cgacgaaggc aggtccacga gctcgaaggt cgcttcggta
     2041 cgcggccagg gacgacctcc cgctggccgg accgcggtca ccacgacatc gtccatcacg
     2101 agttgctcgg ccagcagatc tcgaactcgt gcgaggaagt cgacgtcgtc caccgggaga
     2161 gccttgcatg tttccggtcc tctgccttca gatcgaccca acccgcgccg cggccatcgc
     2221 ccgagcgacg actctgagac catcagctac cggtgggggc ggtgtctccg atgagacgct
     2281 cgtgagagcc gaggcgtagg acctcgctca gcctttgttc cggtcgatgc cggaaccccc
     2341 tcagcctcgg ttgccgctgg gatgaccgcg ggcgcacgct actcctggtt tcagaaccgt
     2401 gcacgcagct cggcacatgc gtgagccgtc agcggcatag agcggacggg tcagtcgcct
     2461 tccacaggct ccgggtcggc gcgatggtct tgctcgacgt cctcacacaa ccagccccaa
     2521 gagttggtca gcacctcttc gcgaagtccg gtgtgtccat gcccgaaggg ccagccggcg
     2581 gtcgcctgcg ggtgctcctg aaagtagagg tgtctcaact catcgttggt cagcgctgaa
     2641 tcacccgacg gctgtgtcgc cagccattcg cacgcgacga caccttcgct caccagcacc
     2701 tgctccgaga ccaagtcata gcgagggctc tcagggtgcc cattcaggtt ctccttcatc
     2761 tccgccagat agcgcgaccg ttgctcagcg gtgtacgtga acgcgctacc cgctggggcg
     2821 ctgttgcccc agtagaaaac cccaagcacg accgccgcaa acgcgagcgc gcccgcgagc
     2881 agacgcggcg tgagacgcag cttccgcatg ttctgagggt aggcgaccat gtgactcggg
     2941 agacccgatt cagctcgatt cgtggcgcgc tcatccggca ggtgagtgca cttcgccacc
     3001 gagagggacc tactcagtta gacctcgaca gcgacaagag tcgagagccg ccgaggggcg
     3061 cgcctgcgcc gcccgcaggc gggttggccg gcgaagccgg ctcttgaatc cttagaggtc
     3121 aactcggcaa gcgtgggctg tcaggtgccg cgataccccg cgacgaagtt cctcgcgatg
     3181 ttcacggtcc atggaaggag atagaagacg acggcgaacg ctacgaaagc aacgccgaac
     3241 acgagccacg cccattcgta gtccaggaga ctcaggactc cgaacactgc caagaacagc
     3301 agtggcggca aggcctcatt caccgcgcga tcgacccacg gcggatgtcc tgtcgtctcg
     3361 atgacgggcc accgagggcg cgcccgctct gcccagtccg cagcagccgc gattcgtcgt
     3421 tccacccgat tcaccgagcg atcctcccag acgagggtcg ttaggcagat tctctgggac
     3481 gtgctccccg gcggggccga caggatgagc caggtctatc ggacgcacgc agatcggcag
     3541 aatcgcgcct ggaggagcac tgtcctgcgt tccaacctgt acttgccgcg tgcctgtcac
     3601 cagtgcctcc ccgtctgtgg gggccggtac cgacaagagg ggaacctgcc gcttacgcag
     3661 ccgacgcagt cgtgtgtcgc cctatcttgg ctgtcgtgga tcggtcgggg cggcaacggt
     3721 cgacgtcgag ccgtgcgcac gctgcggatc cgaccaacag gacgatcggc gctgtcgccg
     3781 tcctgctcgg actggcctgc ctgactgctt gcagcgatcg ccctgagagc ttccagactg
     3841 cttgtgatca cctcgcagcg gcgaacgatg cggtcgcaca tggtgacatc acgcgagcaa
     3901 aggtcgagct caacgatgcc tatgactggg gcgaacctgt atatgaggat gctgccggaa
     3961 ctgaatacgc ggcggccgtc ggcgacttct atcgagctgt cggcatggcg gtttcccact
     4021 tcgggactga ggacgggcag caagccctga acgacgccga gcatgcttgc tcgtagg
//
LOCUS       DATBFZ010000360         1576 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_1233843, whole genome shotgun sequence.
ACCESSION   DATBFZ010000360 DATBFZ010000000
VERSION     DATBFZ010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1233843"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="VH419_10960"
     CDS             <1..432
                     /locus_tag="VH419_10960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044177.1"
                     /translation="LVSITDLADRAPHAMSDGQVLDLGGRRVRWIDTPHVPHGWDAGL
                     IYEEVTGTLFAGDLFTSYGRVAVVTDGDIVGPAIAAEDASHASAITPTTGRIVRNMTT
                     WQPDTLALMHGPAFTGDAVGALTALADDYDRRVSAQLRDFA"
     gene            442..942
                     /locus_tag="VH419_10965"
     CDS             442..942
                     /locus_tag="VH419_10965"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEY7044178.1"
                     /translation="MTDGRPAIQRSVVRLVVLDPENRVLLLLVREPAHPEQGTCWELP
                     GGGIEVGETYLEAAVRELHEETGILARPADVGLPSWRRRATFLHAGTRRLQNEVVVSV
                     RVPGPTVDAAGQLDDEKAIYHGFRWWPVADIEASTERFYPGRLPVLLRRFLAGEQIDE
                     PFEFFS"
BASE COUNT          278 a          545 c          503 g          250 t
ORIGIN      
        1 ctggtgtcca tcaccgattt ggccgacagg gcaccccacg ccatgtctga tggtcaggtg
       61 ctcgaccttg gcggtcggcg agtccggtgg atcgacactc cacacgttcc acacggttgg
      121 gacgccggcc tgatctatga ggaagtcacc ggcacgctgt tcgccggtga cctgttcacc
      181 agctacggac gcgtcgctgt ggtcacggac ggcgacatcg tgggaccggc cattgcagcc
      241 gaggacgcca gccacgccag cgccatcacc ccaaccactg ggcggattgt gcgcaacatg
      301 accacctggc agccggacac tctggcgctc atgcacggtc cagcgtttac cggcgacgcc
      361 gtaggcgccc tgaccgcgtt ggccgacgat tacgatcggc gcgtgagcgc gcagctacgc
      421 gactttgcgt gaggccgcgt tttgacggat ggccggccgg cgatccaacg cagcgttgtc
      481 cgcttggttg tcctcgatcc ggagaaccgg gttctcctgc tgctcgtgcg cgagccggcg
      541 catcccgaac aaggcacgtg ctgggaattg ccggggggtg ggatcgaggt cggggagacc
      601 tacctcgagg cggcggtgcg ggagctacac gaggagaccg gcatcctggc ccggccggcc
      661 gacgttggac tgccgagctg gcggcggcgg gcgacgttct tgcacgccgg gacccgtcga
      721 ctacagaacg aggtagtcgt ctcggtccga gtaccaggac cgaccgtcga tgctgccggc
      781 cagctcgacg acgaaaaggc gatctaccac ggcttccgat ggtggccggt cgccgacatc
      841 gaggcaagca ccgaacgctt ctaccccggc cggctacctg tcctgctgcg ccgtttcctg
      901 gcaggcgaac agatcgacga accgttcgaa ttcttctcct gacgatgcgg cccgctccgc
      961 cggcatccag cgcttaccgc acgcccgctg ctagggcaga cggtccggcg gtacccgctc
     1021 aatgccgatg ccaccacaga ccgccatcac ctcgagcacg tccggcgacg tgtcagcggg
     1081 cacgacaaga tcaaacggca ggtagccgtt cgctggggta ccggcgacct ggcccgcgta
     1141 ggccaatagg tcgtgcgcga catgccgcgg tctcccagca gctccactcc agacggccgg
     1201 cccgcacatc cctccctcat cgcctggggc aagtacccgc accatctccg cgaccgcctg
     1261 ctcgacatct cccgacccca tcgcacacgc cgacgcagca tttcagacga ccgacgcccg
     1321 cgacttgctc agcaatccgg cgccagcatt tgacaagcca tagaagcgtc aacccagccc
     1381 tgcgcattat cgtgacctgg cgcatgatcc acggacatcc gccgagccag tacctcggct
     1441 gcgacgatgg ccgatgagtg ggtcgacgcc ggaagggcgg taaaacgtgg gcactgagcg
     1501 tccgatcgtc ctggttctga cggggccgag ttcggtgggc aagacgagcc tggccaagga
     1561 gatacagcga cgctcg
//
LOCUS       DATBFZ010000361         3180 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_2219625, whole genome shotgun sequence.
ACCESSION   DATBFZ010000361 DATBFZ010000000
VERSION     DATBFZ010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 3180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3180
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2219625"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="VH419_10970"
     CDS             <1..189
                     /locus_tag="VH419_10970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase subunit PaaJ"
                     /protein_id="HEY7044179.1"
                     /translation="FAPPAPREAGEPTLVQLQTGAFRCPYCGSSDTHLENLFGPTPCR
                     SIRYCDACRQPFEQFKTI"
     gene            complement(196..>3180)
                     /locus_tag="VH419_10975"
     CDS             complement(196..>3180)
                     /locus_tag="VH419_10975"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY7044180.1"
                     /translation="LAASFAEIVRSTVPEFAGELLELRGDEALCVFGSARQALRAAVD
                     LQRRLRTPSSDDGSVFPVGVGVGVDAGEAVPTEGGYRGGALNLAARLCSVATPGQILA
                     SEGVTHLAQRVDGVRYSGVRSRRFKGIEAPVRLVEVISETPLPAVPVMPAPKRRRSST
                     AWMVAAGALAVAVVSAIGAVYMRGSDGSSVGLASNSFSLVGSDGSASATLPLDESPFG
                     VAVAGKVAWLPRYSNDAVWAVDTATGQRQSVQVGDGPAAVAVGEGSVWVANALGGTVS
                     EVSPTARTAVGDPIYVGNGPTGIAVGLGAVWVALSVDGAVAKIDPDTARVIHTFSVGT
                     NPTRVAVGFRKVWVTNESVGTVTPIDPATDTVGTPIAVGHGPNGITIGADAVWVTNSL
                     DGSVSRIDPSTFVVATFPTGGDDPQGVAVVGDTVWVAARRSAEIVRLDANTGQIRSNL
                     AVGANPQDVAVVGDGVAITTSTSSKEHRGGTLTIAYAGPGLAKSIDADSFVAWWPEDW
                     EALRMTNDGLVSYKRVPGPDGETVVADLAKALPTPTDDGRTYAFQLREGIRYSTGRPV
                     HASDIRHGLERVFLINARSLKPRPPLDSLFYGAILGVPKCVASPRSCDLSRGIVVNDK
                     TGMVTFHLQHPDPDFLAKLAMPFAVAVPPEVPPHDSGRHPLPATGPYMITRWTKTQHV
                     LVRNPYFREWSPDAQPDGYPNRIAWRVYQEPSQAVTAIEHGAADWLYAFTGIEQQQIH
                     EIETNYAAQIHPSAYPAVDTLAINPNSPLSRDPLARKAIAYAIDRAQVNSILNSGSSV
                     LVRPTCQLVPPNFPGYRPYCRYTLRPGPTWTAPNVALALKLARRSPSYGKPVSVFSWY
                     EPTSGRYIVELLNTLGFRARLVPAKQDGSPTEDDAEVQLFPAATSYVGAADTIDSPDP
                     WPWTQPLTPSDLNDAKAKQSESQYQGTLAWAAVDRRITDFAAVIPLGAHTSLGFTSKR
                     VGNYQFAPAPGNSPIIDQMWVR"
BASE COUNT          504 a         1068 c         1072 g          536 t
ORIGIN      
        1 ttcgcgccgc cggcgccgcg cgaggccggc gagccgacgc tcgtccagct ccagacgggc
       61 gccttccggt gcccgtactg cggctcgtcc gacacgcacc tcgagaacct gttcgggccg
      121 acgccgtgcc ggtcgatccg ctactgcgac gcctgccgcc agccgttcga gcagttcaag
      181 accatctgac aggactcagc ggacccacat ctggtcgatg atgggcgagt taccgggcgc
      241 gggcgcgaac tggtagttgc cgactcgttt ggaggtgaac ccaaggctcg tgtgcgcgcc
      301 gagtgggatg acagccgcga agtcggtgat gcgtcgatcg acggccgccc aggccagcgt
      361 gccttggtat tgactttcgc tctgctttgc cttggcgtcg ttcaggtcgc tcggggtgag
      421 tggctgggtc catggccagg ggtcaggcga gtcgatggtg tccgcggctc caacgtagct
      481 agtcgccgcg gggaacagtt ggacttccgc gtcgtcttct gttggcgacc cgtcttgttt
      541 tgccggcacg agccgcgcgc ggaacccgag ggtgttcaga agttcgacga tgtaacggcc
      601 ggaagtcggc tcgtaccagg agaagaccga gaccggtttg ccatagcttg gcgagcggcg
      661 ggcgagcttc agggcgagcg ccacgttcgg tgctgtccat gttgggccag gtcggagggt
      721 gtatcgacag taaggtcgat accccgggaa gttgggtggg accagctggc acgtcggtcg
      781 cacgaggacg ctgctgccgc tgttcaggat gctgttgacc tgggcgcggt caatggcgta
      841 tgcgatcgcc ttccgtgcta gcggatcgcg actcaacggt gagttcggat tgatggcgag
      901 cgtgtcgact gcggggtagg cggatgggtg gatctgggct gcgtagttgg tttcgatctc
      961 gtggatctgc tgctgttcga tacccgtgaa cgcgtagagc cagtcggcgg cgccgtgctc
     1021 gatcgcagta acagcctggc tgggttcctg gtagacccgc caggcgatgc gattgggata
     1081 tccgtcgggc tgcgcgtccg gcgaccactc gcggaagtac gggttgcgca caaggacgtg
     1141 ctgcgtcttt gtccagcggg tgatcatgta ggggccggtg gcaggcagtg ggtgacggcc
     1201 ggagtcgtgc ggcgggactt cggggggcac ggcgaccgcg aagggcattg ccagtttcgc
     1261 gaggaagtcg ggatcgggat gttgcagatg aaacgtgacc atcccggtct tgtcgttcac
     1321 cacgatcccg cggctgaggt cgcacgaccg cggggaggcc acgcacttcg gtacgccgag
     1381 gatggcgccg tagaagagcg agtccaaggg cggccgaggc ttaagagaac gggcattgat
     1441 caaaaagacg cgctcgaggc cgtggcggat gtcgcttgca tggactggtc tcccggttga
     1501 gtacctgata ccttctcgta gctggaaggc ataggtgcgg ccatcgtcgg tgggcgtcgg
     1561 aagcgcctta gccagatccg cgaccaccgt ctccccatcc ggaccgggta cccgtttgta
     1621 actgacgagg ccgtcgttcg tcatcctaag cgcttcccag tcctcgggcc accacgcgac
     1681 gaagctgtcg gcgtcgatcg atttggcaag cccaggccct gcgtacgcga tggtcaacgt
     1741 tccaccccgg tgctccttcg aggatgtcga ggtggtgatc gccacgccgt cgccgaccac
     1801 cgccacatcc tgcggattgg cgcccacagc caggttcgac ctgatctgtc cagtattggc
     1861 gtcgaggcgg acgatctccg cggagcggcg ggcagccacc cagacggtgt caccgaccac
     1921 cgccacgccc tgcgggtcgt cgcctccagt cgggaacgtg gctaccacga atgtgctggg
     1981 gtcgatgcgc gagaccgacc cgtccaggct gttggtcacc cacaccgcgt cggctccgat
     2041 ggtgatgccg ttgggtccgt ggccgaccgc aatcggcgta cccacggtat ctgtcgccgg
     2101 atcgatcggc gtcaccgtcc cgaccgactc gttggtgacc cacaccttgc ggaagccgac
     2161 agcgactcgg gtggggtttg ttccgaccga aaacgtatga atgacccgag cggtgtccgg
     2221 gtcgatcttc gcgaccgcgc cgtcaacgct cagcgccacc cacaccgccc ccagaccgac
     2281 cgcgatcccg gtcgggccgt tccccacata gatcggatca ccgaccgccg tccgggcagt
     2341 cgggctcacc tccgacaccg tgccaccgag tgcattcgca acccacacgc tgccctcgcc
     2401 gacggcgact gcagccggcc cgtcgccaac ctgaacagac tgccgctgtc cggtagccgt
     2461 gtccacggcc cagacggcgt cgttcgaata gcgcggcagc cacgcgacct tcccggcgac
     2521 cgctaccccg aacggcgact catccaatgg cagggtcgcg ctcgcgctcc cgtccgaccc
     2581 gaccaggctg aacgagttgc tcgccaggcc gaccgatgac ccgtcgctcc ccctcatgta
     2641 gacggcaccg atcgcggaca ccacggccac cgcgagcgct ccggccgcga ccatccacgc
     2701 cgtcgacgat cgccgccgtt tcggtgcggg catgaccgga acagcgggga gcggggtctc
     2761 cgagatgacc tcgaccagcc gcaccggcgc ctcgatcccc ttgaaccgtc gtgaccggac
     2821 gcctgaatag cgcactccgt cgacgcgctg ggcgaggtgg gtgacgccct cgctggcgag
     2881 gatctgaccc ggcgtcgcga ccgagcacaa cctcgctgcc aggttcagtg cgccgccccg
     2941 gtacccgccc tcggtcggca ccgcctcgcc cgcatccacc cccactccga ccccgaccgg
     3001 gaagacgctg ccgtcgtcgc tcgagggggt gcgcagcctc cgctggaggt cgaccgccgc
     3061 ccgcagcgcc tgccgcgccg agccgaacac gcacagggcc tcgtcgccgc gcagctcgag
     3121 cagctcgccc gcgaactccg gaaccgtgct tcggacgatc tcggcgaacg acgccgccag
//
LOCUS       DATBFZ010000362         3693 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_3732440, whole genome shotgun sequence.
ACCESSION   DATBFZ010000362 DATBFZ010000000
VERSION     DATBFZ010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 3693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3693
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3732440"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(457..978)
                     /locus_tag="VH419_10980"
     CDS             complement(457..978)
                     /locus_tag="VH419_10980"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEY7044181.1"
                     /translation="MRSVRHGSAVIDFPNELEIVITRDFDAPIALVFDVLTKPEHVRH
                     WGATPPDKMLECSIDLRVGGNYRSSFLTGDGQVFTIRGTYLEVEPPTRTRQTWLFEMW
                     PDVDAVETFELHETGGVTTFRWSLAFQDKAGRDHMTGFDQDEITGAGQLDSLDAMEDI
                     LNSLLNPRDTATQ"
     gene            complement(975..1316)
                     /locus_tag="VH419_10985"
     CDS             complement(975..1316)
                     /locus_tag="VH419_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009073927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY7044182.1"
                     /translation="MARAATTSDVFNAVADVHRREILDLLITGEQAVGTIVGDLSLSQ
                     PQVSKHLRVLNEVGLVTRRVEGRFRLYRLAPENLRPMHDWVVKYERVINNRLDRIDDY
                     LQELQRQGAPQ"
     gene            complement(1552..2292)
                     /locus_tag="VH419_10990"
     CDS             complement(1552..2292)
                     /locus_tag="VH419_10990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044183.1"
                     /translation="MHPLVRCVAGFFAVSALLVPGFGAVDLSVSWDLYWQPVIAGTWG
                     VFFSLLVAFPFLVIACRPAASPGAVWTLLIASASMLVSAVASLELQLAGLLLWFGLGI
                     AVVALPSRASWWPLVLQPHRTAALVAVVSSTPWLAYAWHMASRNRQGHPADISSGVDH
                     YSVIAALAVALAALAVLTTLSPVGRRQIGTNVGVCAAYLGALSYNWPNITASLGRTGG
                     IAAIVWGLLLAVWAWLPTRTPEVQTALE"
     gene            complement(2538..3311)
                     /locus_tag="VH419_10995"
     CDS             complement(2538..3311)
                     /locus_tag="VH419_10995"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY7044184.1"
                     /translation="MGERLDYAFPHYSDDEGRRLDLFQERLDPLTIRRIERFGISRDA
                     ACLEIGAGRASITRWLANRVAPEGRVTATDVQPEVLAAIDMPNVDVLRHDIRTDTFPA
                     ESFDLVHTRAVLMHVPADTALLQRMVSWLRPGGWLLLEEPDFGMWEADSDPLWALHPQ
                     AWHRAFPNGSRSRGRWLLGQIPRLGLAHVGADAEVDIVAPGTPLAEFHRLSMEAIYPA
                     ALAAGALTADQATALLQRPTQPDFLSFGFVHVGVWGRKP"
BASE COUNT          660 a         1194 c         1245 g          594 t
ORIGIN      
        1 atcgtgccga actccgaccc gtttccttgc cggtcggttg ttgctcgccg cacgtttggg
       61 ctaatcgcga caaccaccat caactgggaa gagccgcaaa cggctcatgg ggcaggcggc
      121 tccggggatc gacgccttct aggccctcct cgagcagggc tccaccgagc ggtttgccca
      181 ccatcctgac cggatggcct ctggtgcaat ggtgccgacc tcgaacgaga tccatccgga
      241 cgacgtcgcc ttgttctacg ccggcgtcga agcaaagctg gtgacgatgt gtcgcggggg
      301 cggttcaaca tgcgggaccg cctcccacgg aggcgtcact cggccgggag ccgccgcgct
      361 tgaatcacac gcttccggtg agcgatcgat gcgctcggcc atcgatcatc gtcagtgctg
      421 cgcccggcca gccaccttgt tccgcgtcgc ggccagttac tgagtggcgg tgtccctcgg
      481 gttgagaagt gagttgagga tgtcttccat cgcgtcgagg ctgtccagct ggccagcccc
      541 cgtgatttcg tcctgatcga acccggtcat gtggtcacgg ccggccttgt cctggaacgc
      601 cagactccat ctgaacgtcg tgacgccgcc ggtctcatgc agctcgaagg tctcgaccgc
      661 gtccacgtcc ggccacatct cgaacagcca cgtctgccgg gtccgggtcg gcggctcgac
      721 ctccaagtag gtcccgcgga tggtgaacac ctgtccgtcc ccggtcagga acgacgagcg
      781 gtagttcccg ccgacgcgga ggtcgatcga gcactccagc atcttgtccg gaggcgtggc
      841 gccccagtga cggacgtgct cgggcttggt cagcacgtcg aagacgagag cgatcggcgc
      901 gtcgaagtcc cgcgtgatca cgatctcgag ctcgttgggg aagtcgatga cggctgaacc
      961 atgccgaaca gacctcactg tggcgctcct tgtcgttgta gttcctggag gtagtcgtcg
     1021 atccgatcga gccggttgtt gatgacccgc tcgtacttca ccacccagtc gtgcatgggg
     1081 cgaagattct ccggagccag gcggtacagg cggaagcgac cctccacccg gcgcgtgacc
     1141 agtcccacct cattgagcac gcgaaggtgc ttcgagacct gaggctgcga caacgagagg
     1201 tcgcccacga tcgtcccaac cgcctgctcg ccggtgatca gcaggtcgag gatctcgcga
     1261 cggtggacgt cggcaaccgc gttgaagacg tctgaggttg tcgctgcgcg ggccaccaag
     1321 ccaatatatt ccgatatcaa catgtgtcaa ccaagtggcc gcgttccgcc gcgacgaggc
     1381 gcgcgccact ccgccgggac ggcgaggtac ggcggagact gcgtcgcatc gtgggcggcc
     1441 cgcgccggct ggcacgcccg ctggaccgac gacgtccgcc ggatccggcc atgcacgacg
     1501 atggtctcga cgtgagagac ggtgtgcccg cgcggcgtaa tgccgagcac ctcactcgag
     1561 ggcggtttgg acctcggggg tgcgggttgg aagccatgcc cagacagcga ggagcagccc
     1621 ccagacaatc gcggcgatgc caccggtccg tcccagggat gcggtgatgt tcggccagtt
     1681 gtaggagagg gctcccaggt acgccgcgca cacccccacg ttggtgccga tctgtcggcg
     1741 ccccaccggt gacagtgtcg tcaggaccgc gagtgcggcg agtgccaccg cgagggccgc
     1801 gatcaccgag taatggtcca ctcccgagct gatatcggcc ggatgcccct gcctgtttcg
     1861 tgacgccatg tgccaggcgt aggccaacca aggggtggag ctgacgacgg cgacgagcgc
     1921 cgccgttcgg tgcggctgga gcaccagcgg ccaccaggag gcccgggacg gaagggccac
     1981 caccgcgatt cccaggccga accagagcag cagccccgcc aactgaagct cgagcgacgc
     2041 caccgccgag acgagcatgg acgcggacgc gatcagcagc gtccacacag cgccaggtga
     2101 cgccgcgggc cgacacgcga tgaccaggaa cgggaacgca acaaggagcg agaagaacac
     2161 cccccaagtg ccggcgatca ccggttgcca gtacagatcc cagctgacac tcaggtccac
     2221 ggcaccgaaa cccggcacca gcaacgctga gaccgcgaag aaaccggcca cacaacggac
     2281 gagaggatgc acgactcgca tgctctcagg aagacgaaac ccggtcagtg ctttctggaa
     2341 ggctgggcag tcaggtacgg gaaaggacga tcacacgcgg tcatcgcggg gtgagtcagg
     2401 cgaagacctc ggtgcgaact cccactggat gttgtcgggg tctcggaacg tgatcatggc
     2461 cagtgggccg cgcgatccat gtggactccg actgtcgcag cgtaaggatc caaggtcgtc
     2521 agcacggcat gtgagagtca tggtttgcgt ccccaaacac cgacatggac gaacccgaag
     2581 gagaggaagt ccggttgagt cggacgctgg agcagtgcgg tggcctggtc ggctgtgagt
     2641 gcgccggctg cgagcgctgc ggggtagatc gcctccatgc tgaggcggtg gaactccgcg
     2701 agcggagtcc cgggtgcgac gatgtcgacc tcggcgtcgg cgccgacatg tgcaagaccg
     2761 agcctcggga tctgtcccaa cagccaccgg ccgcgagacc gggagccgtt cgggaacgca
     2821 cgatgccagg cctgcggatg tagcgcccac agcggatcgc tgtctgcttc ccacatgccg
     2881 aagtcgggct cctccaggag cagccaacca ccaggccgga gccaggagac catccgttgg
     2941 aggagtgcgg tgtcggctgg tacgtgcatc agtaccgctc gtgtgtggac gaggtcgaac
     3001 gactcggctg ggaaggtgtc ggtgcggatg tcgtgccgca gcacatcgac gttcggcatg
     3061 tcgattgctg cgaggacctc cggttgcaca tcggttgccg tcacgcgtcc ttccggtgcg
     3121 acccggttgg ccagccaccg ggtgatcgag gcgcgaccgg ccccgatctc gagacacgct
     3181 gcgtcgcgac tgatgccgaa gcgttcgatc cgtcggatgg tgagcgggtc gaggcgctcc
     3241 tgaaacaggt cgagccggcg gccctcgtcg tcggagtagt gcggaaacgc atagtcgagt
     3301 cgctcgccca cccatgaatc ctgcccctcc cggcccccgg agtcaccccg gctgatgact
     3361 gagaaaggca tggtcaacaa ggctcgagcc acgttctcaa cagtcgccgt ggtcgcgccg
     3421 gccgccgtgg accgtgcatc gcccgcctga gaccgcgcgt acggtcagcg gctcagtgcg
     3481 ttcactacca gagttcgtcg ccgccggtcc cgaggtaccg gctgctcagg gagagatttc
     3541 tcccggcgga agggcgcggg tgccgacggc ggtggcgtga tgcgatgagg tgcggtcacc
     3601 tcagtcccag cggctgatct ctccctccgg gaaggggtga aagccgtcgc cttctgcgcc
     3661 ggtcttgagg ctggtcaggt aggcgcccca gtt
//
LOCUS       DATBFZ010000363         2108 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_2220963, whole genome shotgun sequence.
ACCESSION   DATBFZ010000363 DATBFZ010000000
VERSION     DATBFZ010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2220963"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="VH419_11000"
     CDS             <1..417
                     /locus_tag="VH419_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020386171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 2,3-aminomutase"
                     /protein_id="HEY7044185.1"
                     /translation="QIMPYYFYMCDMIPYSEHWRVSVADAQRLQHAIMGYLPGFATPR
                     IVCDVPFVGKRWVHQLSDYDTVRGISYWTKNYRTSIEAEDPEALSRHYEYYDPIHTLP
                     REGQDWWAEHGQLDESALKAAEVAEASRRTAALQAH"
     gene            596..1591
                     /locus_tag="VH419_11005"
     CDS             596..1591
                     /locus_tag="VH419_11005"
                     /inference="COORDINATES: protein motif:HMM:NF024734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HEY7044186.1"
                     /translation="MPPKEKSSGMDIDWDGDAGVFLRRDALAAGCDDKTLARMVRAGL
                     LHRIRRGAYIDASVWAGLDAVGRHRMTARAVLRTAHPSAVLTHVSALVEQGVPVWNVD
                     LGEIHLTRTDARSGRREAGVVHHRGALLPEEVLTCHGVQVSMPARAIIELSTMAGVES
                     VLVSSNWLLHRGATTREELARVAKAFECWPGSLRSDLAVRLSDGRCAWPGEARTSYLI
                     WRHHLPRPVPQYEVHDEGGNVVALLDFAFPEYGVFLEFDGAIKYERLRRRGETVEDVI
                     RREKRREELVCLLTGWICIRVTWEDLARPQATAARIRRLLDTRRRHGSPRVVDPS"
     gene            complement(1658..2098)
                     /locus_tag="VH419_11010"
     CDS             complement(1658..2098)
                     /locus_tag="VH419_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016669904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEY7044187.1"
                     /translation="MEDTDRTIVRLLAADGRMSFTDLGKATGLSTSAVHQRVKRLEKR
                     GIITGYGASVNHEGVGLALTAFVSITPIDPSQPDDYPDRIRGIKAIESCWSVAGEESY
                     ILKVRVETPHALEDLLARIRADANVSTRTTIVLSTPFENRTVTE"
BASE COUNT          333 a          717 c          723 g          335 t
ORIGIN      
        1 cagatcatgc cgtactactt ctacatgtgc gacatgatcc cgtactccga gcactggcgg
       61 gtctcggtcg ccgacgccca gcggctccag cacgcgatca tgggctacct ccccggcttc
      121 gccaccccgc gcatcgtctg cgacgtgccg ttcgtcggga agcgctgggt gcaccagctc
      181 tccgactacg acaccgtgcg tggcatctcg tactggacca agaactaccg gacctccatc
      241 gaggccgagg accccgaggc cctcagccgc cactacgagt actacgaccc gatccacacc
      301 ctcccccgcg agggccagga ctggtgggcc gagcacggcc agctcgacga gtccgccctg
      361 aaggccgccg aggtcgccga ggcctcccgc cggactgccg ccctccaggc ccactgaggg
      421 ggccctttgc cggtttcccc gcctgagcgg gggaatcgga cgttggaggg gtttgcaacc
      481 cgcacaaagg ggccggacag ggccccaagg ctgtgacggg tgggacgtcg cgcgggggta
      541 cgaacccagc cttcgcaggc gttgtgcaca ggtgcgtcgg gcccgggttt ccgccgtgcc
      601 tccgaaggag aaatcctcgg gcatggacat cgactgggac ggggacgccg gcgtcttcct
      661 acgtcgcgac gcgctcgctg ccggatgcga cgacaagacc ctggcccgca tggttcgagc
      721 cggattactc caccgcatcc gacgaggtgc ctacatcgac gccagcgtct gggccgggct
      781 ggacgccgtc gggcgccaca ggatgaccgc tcgtgccgtc ctgagaacgg ctcatccttc
      841 cgccgtcctc acccacgtgt ctgcgctcgt cgagcagggg gtgccggtct ggaacgtcga
      901 tctcggcgag atccatctga cccgcaccga tgccaggtcc gggcgacgtg aagcgggagt
      961 cgtccaccat cgtggcgcgc tgttgccgga agaggtcttg acctgtcacg gagtccaggt
     1021 gtccatgccg gcgcgggcga tcatcgagct ctcgaccatg gcgggagtcg agtccgtgct
     1081 ggtctcctcg aactggctgc tccaccgcgg cgcgacgacg cgcgaggaac tggcccgcgt
     1141 cgccaaggcg ttcgagtgct ggccgggaag cctcaggagc gacctcgccg tcagactcag
     1201 cgacgggaga tgtgcgtggc cgggtgaggc gcgcacgtcg tacctgatct ggcgacacca
     1261 ccttcccagg ccggtccccc agtacgaggt ccacgacgaa ggcggaaacg ttgtggcact
     1321 gctcgacttc gcgttccccg agtacggcgt gttcctggag ttcgacgggg cgatcaagta
     1381 cgagcggcta cgccgaaggg gcgagaccgt ggaggacgtg atcagacggg agaaacgccg
     1441 tgaggagctc gtctgcctgc tgaccgggtg gatctgcatc cgcgtcacct gggaggacct
     1501 ggcccgtccg caggccactg cggcccgaat ccgacgcctg ctcgacaccc gacgtcgtca
     1561 cggcagcccc agggttgtcg acccttcctg accgttcgca cgggttgcaa ccgtcacgaa
     1621 gggccgactc ccccgcctga gcgggggatc cgggcgccta ctccgtgacc gtgcggttct
     1681 cgaacggcgt gctgaggacg atcgtggtgc gggtggagac gttggcgtcg gcgcggatgc
     1741 gggcgaggag gtcttcgagg gcgtgggggg tctcgacgcg gaccttgagg atgtaggact
     1801 cctcccctgc caccgaccag caggactcga tggccttgat cccacggatg cggtcggggt
     1861 agtcgtcggg ctggctgggg tcgatcggcg tgatcgagac gaacgccgtc aacgcgaggc
     1921 cgaccccttc gtggttcacc gacgcgccgt agccggtgat gatgccgcgc ttctcgagcc
     1981 gcttgacccg ctggtgcacc gccgaggtcg acagaccggt cgccttgccc agatcggtga
     2041 aagacatccg gccatcggcc gccaacagcc ggacgatggt gcggtcggtg tcctccacgc
     2101 cgacgacc
//
LOCUS       DATBFZ010000364         6609 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_1236264, whole genome shotgun sequence.
ACCESSION   DATBFZ010000364 DATBFZ010000000
VERSION     DATBFZ010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 6609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6609
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1236264"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            32..493
                     /locus_tag="VH419_11015"
     CDS             32..493
                     /locus_tag="VH419_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012918130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEY7044188.1"
                     /translation="MSTPEARLRELGLAVPDVAKPVAAYVPAVRTGNHVYTAGQLPLR
                     DGALITTGKVGGEVTAEDATACAEQCALNGLAAVKSVVGDLSRIVRVVKLVAFVASTP
                     DFTGQPGVANGASDLIGKVFGDAGQHARSAVGVPVLPLDAPVEVEMVVEVS"
     gene            496..1347
                     /locus_tag="VH419_11020"
     CDS             496..1347
                     /locus_tag="VH419_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012918131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY7044189.1"
                     /translation="MGATPVHATRRLSPELVEHARSFLAGERRAVPPRPAATVVLLRD
                     ADAGPEVYLLRRHRRMAFAAGMYAFPGGTVDPRDADADVAWAGPEPATWAAVLACDER
                     EARALVCAAVRETFEESGVLFAGTSASSIVSDTTGEGWEQDRGALVDRSLAFGELLDR
                     RGLVLRSDLLVAWAHWITPEFEPRRYDTRFFLATMPAGQRTRDVSGEADTVTWMSPAA
                     AVAGVDDGDMAMLPPTYVTVKELAAYTSSDKAWAAGIGRRITTIIPGVELEGGEGFLT
                     LDESVLP"
     gene            1344..2159
                     /locus_tag="VH419_11025"
     CDS             1344..2159
                     /locus_tag="VH419_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008356262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY7044190.1"
                     /translation="MTGAWTGGVFGHAQCVLADNPSPMTLDGTNTWVLRADDDAGSIV
                     IDPGPLDAHHLSAVADVAGHVAGVLLTHGHLDHSEGARRFAELVGCGVRALDPSFVYG
                     DEGLHDGDVVEVDGLEVRVVGTPGHSSDSLSFVLPVDDAVLTGDTVLGRGTTVVAHPD
                     GALGAYLGSLRRLRDLTSQQGLTTVLPGHGPVLGDALSVLDHYLAHRAERLEQVRDAV
                     AHLSAAPDGGAVSGQVEPMDVVRLVYADVDEQLWPAAELSVRAQLDYLREALS"
     gene            complement(2181..2828)
                     /locus_tag="VH419_11030"
     CDS             complement(2181..2828)
                     /locus_tag="VH419_11030"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY7044191.1"
                     /translation="MRITHLGHACILLEHNGSRLLLDPGTYSSGLEGLTGLTAIAVTH
                     QHPDHVDPERFAALTETNGEAELIVETATVEALGLGGRRLTRVEAGHAARVAEMEFRA
                     VGGRHARNHDQVPPLGNVGYVVTANGTTFFHPGDSYDEAPGDIDVLGLPLNAPWCRMS
                     ETLSFLHAVSPRVVVPIHTGLLSEDGLAAYLMHVQRFGPAGTEVVDLRPGSAYDV"
     gene            complement(3012..3689)
                     /locus_tag="VH419_11035"
     CDS             complement(3012..3689)
                     /locus_tag="VH419_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017933295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY7044192.1"
                     /translation="MNTDVLRQTPLFSGLDDEAAEALRSSMTGAKVRRGDVLFREGDA
                     GDRLYVVIDGKVKLGRTSADGRENLLAILGPGQMFGELSLFDPGPRSATVTAVTDCTM
                     QSLSHDELGRWLNGRPEVARGLLTQLAGRLRKANDVVADLVFSDVPGRVAKALLDLSS
                     RFGRVADDGVHVHHDLTQEELAQLVGASRETVNKALADFASRGWLRLEARSVVLMDVE
                     RLKRRAR"
     gene            3800..4531
                     /gene="nth"
                     /locus_tag="VH419_11040"
     CDS             3800..4531
                     /gene="nth"
                     /locus_tag="VH419_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011753835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEY7044193.1"
                     /translation="MSAGADVAVTSRSESRTALVRRARRMNRLLAETYPDARCELDFT
                     NPWELLVATVLSAQTTDKRVNLVTPVLFGTYPGPAELAAADPEEVEQIIASTGFFRAK
                     TASIMKMAAAVVDTYDGEVPGRLDDLVTLPGVGRKTANVVLGNAFDVPGITVDTHFAR
                     LVRRFGWTDETHPVKVEHAVGALFPKRDWTMLSHHLIWHGRRRCHARNPACGACPLDR
                     LCPSYGEGPTDPVKAAKLVRTEGRA"
     gene            4528..5205
                     /locus_tag="VH419_11045"
     CDS             4528..5205
                     /locus_tag="VH419_11045"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEY7044194.1"
                     /translation="MIARHLRYGSHSATPVRLAVVAAAGAVTASLLVSCSSSTPHVDA
                     YTYNPEKAAVDVDTADLRRQKAIAGIPNCPRSTQGATQQDKGLPPITLPCLGGGRDVD
                     LAGLTGPPTVLNFWAQTCGPCRQESPLFQRLHDSGAVRVVGVDFLDPLPDRAIAFAKE
                     LGLTYPQVADPEGATRAPLHVSGLPMTMFVDSQGVVRHVEYGAVRSAASLAALVSTYL
                     AVHVDLG"
     gene            5210..5983
                     /locus_tag="VH419_11050"
     CDS             5210..5983
                     /locus_tag="VH419_11050"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007078971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HEY7044195.1"
                     /translation="MIALPPGADDRRRGRTGDLPQWLQLLSDVVGSIRVGQLTSFLPP
                     RDADPRPAAVLMLFGESSGEPDVLLLERAHTMRSHAGQVAFPGGVLDPDDADAVAAAL
                     REAQEETGLDPSGVEVLGILPSLWLPPTNFAVTPVVGWWRTPTPVAVVDPAETASVHA
                     VPIAELADPANRATVRHPSGYLGPAFLVRDLVVWGFTAGLLARIFGIVGWERPWDDSR
                     IVDLPADLVSSSLRDLERAAESGALPPSRPPGSGPESPR"
     gene            5980..>6609
                     /locus_tag="VH419_11055"
     CDS             5980..>6609
                     /locus_tag="VH419_11055"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEY7044196.1"
                     /translation="MNALDWILILFAVLYALSGYQQGFIVGAASTIGLLAGGFIGVQV
                     TPWLLDGFDPALSVSVAALLVVLACAFVGQAIGAYVGGALRGRLTWQPARVLDACSGA
                     ALSVVAMLLIAWVLGVAASGADLRGLNNEVRTSSVLAGVDKALPGGSDRVLSAFNSLV
                     DSSLFPRYLEPFTPEHIKEVPPPNRGVVAKPGVRAAEDSVVKILGAADSC"
BASE COUNT          903 a         2316 c         2340 g         1050 t
ORIGIN      
        1 gcctacttca agcggccgaa ggagtcctga cgtgtcgacg cctgaggcgc ggctgcgcga
       61 gctcggcctc gcggtgcccg acgtcgccaa gcccgtcgcg gcgtacgtgc ctgccgtgcg
      121 gaccgggaac cacgtctaca ccgccggcca gctgccgctg cgtgacggcg cgttgatcac
      181 gacgggcaag gtgggtggcg aggtcactgc ggaagacgcc accgcatgcg ccgagcagtg
      241 tgcgctcaac ggcctcgccg ccgtgaagtc cgtggtcgga gacctgtcgc gcatcgtgcg
      301 ggtggtcaag ctcgtcgcgt tcgtggcgag tacgccggac ttcaccggcc agcctggggt
      361 ggcgaacggt gcgagtgacc tcatcggaaa ggtgttcgga gacgccggtc agcacgccag
      421 gtcggccgtc ggcgtacccg tgctgccgct ggacgcgccc gtcgaggtcg agatggtggt
      481 cgaggtctcc tgactgtggg tgcgacgccc gtccacgcaa cccggcggct gtcgccggag
      541 ctggtcgagc atgcccggtc ctttctggcc ggtgagcgcc gcgcggtgcc gccgagacct
      601 gccgccacgg tggtcctgct gcgcgacgcg gacgccggcc cggaggtcta cctgctacgg
      661 cggcaccgac ggatggcgtt cgccgccggg atgtacgcgt tcccgggcgg caccgtcgat
      721 ccgcgcgacg ccgacgcgga cgtcgcgtgg gccgggcccg aaccggcgac ctgggcagcg
      781 gtactcgcct gcgacgagcg cgaggcccgc gcgttggtgt gcgcggccgt gcgggagacc
      841 ttcgaggagt cgggagtctt gttcgccggt acgtcggcct cgtcgatcgt ctccgacacc
      901 acaggtgagg gctgggagca ggatcggggc gcgctggtgg accgcagtct ggcgttcggc
      961 gagctcctcg accgccgcgg tctcgtgctg cgctccgacc tgctggtcgc gtgggcgcac
     1021 tggatcacgc cggagttcga gccgcgccgg tacgacaccc gcttcttcct ggcgacgatg
     1081 cccgccggcc agcgcaccag ggacgtgtcg ggagaggccg acacggtgac gtggatgtcg
     1141 ccggcagccg ccgttgcggg agtcgacgac ggtgacatgg cgatgcttcc gccgacctac
     1201 gtcacggtga aggagctggc ggcgtacacc tcgtcggaca aggcctgggc tgctggcatc
     1261 ggtcggcgca tcaccacgat catcccgggt gtcgagctcg agggcggcga gggtttcctc
     1321 acgcttgacg agagcgtcct gccgtgacgg gcgcgtggac cggcggggtg ttcggccacg
     1381 cgcagtgcgt gctcgcggac aacccgtcgc cgatgacgct ggacggcacg aacacgtggg
     1441 tgctacgggc tgatgacgac gctggctcga tcgtcatcga ccccggacct ctcgacgcgc
     1501 accacctgag cgccgtggcc gacgtcgccg gtcatgtcgc cggggtgctc ctgacgcacg
     1561 ggcacctcga ccacagcgag ggcgcgcggc ggttcgctga gctggtcgga tgcggcgtgc
     1621 gggcgctcga cccgtcgttc gtgtacggcg atgagggcct gcacgacggc gatgtcgtcg
     1681 aggtggacgg tctggaggtt cgcgtcgtcg gcacgcccgg tcacagcagc gactcgctca
     1741 gcttcgtcct gccggtcgac gatgccgtgc tgaccggcga caccgtgctc ggccgaggca
     1801 cgaccgtggt cgcgcatccg gacggagccc tcggggcgta cctcggctcg ctccgtcggc
     1861 tgcgtgatct gacctcgcag caggggctca cgacggtgct gcccggccat ggtccggtgc
     1921 tcggcgatgc gttgtcggtg ctcgaccact acctcgccca ccgggcggaa cggctggaac
     1981 aggtccgcga cgcggtcgct cacctgtccg cagcacccga cggcggtgcg gtgtcgggtc
     2041 aggtcgagcc gatggacgtc gtgcggctgg tctacgccga cgtcgacgag cagctctggc
     2101 cggccgccga actcagcgtc cgggcccagc tcgactacct ccgggaagcc ttgtcgtagc
     2161 ggctggtcgg tggcgagccg tcagacgtcg tacgccgaac ctggccgcag gtcgacgacc
     2221 tcggtgccgg ccggcccgaa gcgttggacg tgcatcaggt acgccgcgag gccgtcctcg
     2281 ctgagcagac cggtgtggat ggggaccacc acgcgagggg agacggcgtg gaggaacgag
     2341 agcgtctcgc tcatccggca ccacggcgcg ttcagtggca gaccgaggac gtcgatgtcg
     2401 ccgggcgcct cgtcgtagct gtcgccgggg tggaagaacg tcgtgccgtt ggccgtcacc
     2461 acgtacccga cgtttcccag cggtggcacc tggtcgtggt tgcgcgcatg ccggccacct
     2521 accgcacgga actccatctc ggcgacgcgg gccgcgtgac ccgcctcgac tcgcgtgagg
     2581 cgacggccgc cgagcccgag tgcctcgacg gtcgccgtct cgacgatgag ctcggcttcc
     2641 ccgttggtct cggtcaacgc agcgaagcgt tccgggtcga cgtggtcggg gtgttgatgc
     2701 gtgacggcga tggccgtgag gccggtgagt ccctccaagc cggaggagta cgtccccggg
     2761 tcgagcagca gccgagatcc gttgtgctcg agcaggatgc aggcgtgtcc gaggtgggtg
     2821 atccgcatgc ctcgcacgtt agtaccccgt caaccaacat tcgctctgtt gcgcgccgcc
     2881 cgggcggcga tctgcggcgt tatcgtcggt caacgatgct gcgcatcgcc tccctcctct
     2941 gccttgcagc tcatccaccc ggacaccgct cacgacgagc aacgttggtt gacgcggcac
     3001 tagcggagga gtcagcgggc gcggcgcttg aggcgttcga cgtccatcag cacgaccgaa
     3061 cgagcctcca gccgcagcca gccacgggag gcgaagtccg ccagtgcctt gttgaccgtc
     3121 tcccgcgacg cgccgaccag ctgggccagc tcctcctgcg tcaggtcgtg atgcacgtgc
     3181 accccgtcgt cggccacccg gccgaatcgg gaggacaggt cgagcagcgc cttcgccacc
     3241 cgccccggca cgtcggagaa cacgaggtcg gcgaccacgt cgttcgcctt gcgcagccgt
     3301 ccggccagct gcgtgagcaa ccctcgcgcg acctcgggac gcccgttgag ccagcggccg
     3361 agctcgtcgt ggctcaggct ctgcatcgtg cagtcggtca ccgccgtcac cgtcgccgac
     3421 cggggaccgg ggtcgaagag cgacagctcc ccgaacatct ggccaggccc gaggatcgcc
     3481 agcaggttct cccgcccgtc agccgacgta cgaccgagct tgacctttcc gtcgatgacg
     3541 acgtacagcc ggtcaccggc atccccttcg cggaacagaa cgtctccgcg gcgcaccttc
     3601 gccccggtca tggaggaccg cagcgcctcg gccgcctcgt cgtcgaggcc gctgaacaag
     3661 ggcgtctgac ggagtacgtc ggtgttcacg tcttcctcct cgccgtccac accgggcgtg
     3721 tggcccgcat cacagtctgt cgcattcgcc cgcgcgaacg cgagccgggc cgtcgtggcc
     3781 acaccgtagg ctggaggcca tgtccgccgg tgccgacgtg gccgtcacgt cacgatcgga
     3841 gagccgcacc gcgctcgtcc ggcgagcccg ccggatgaat cgcctccttg ccgagacgta
     3901 ccccgacgcg cgatgcgagc tggacttcac gaacccctgg gagctgctcg tcgcgaccgt
     3961 gctgtccgca cagacgaccg acaagcgggt caacctcgtc acgccggtgc tcttcggcac
     4021 gtaccccgga ccggccgagc tcgcggccgc cgatcccgag gaggtcgagc agatcatcgc
     4081 ctcgaccggg ttcttccgcg cgaagaccgc ctcgatcatg aagatggcgg cagccgtcgt
     4141 cgacacgtac gacggagagg tcccgggcag gctcgatgat ctcgtgacgc ttcccggcgt
     4201 cggccgcaag accgcgaacg tcgtactcgg caacgccttc gacgtgcccg gcatcacggt
     4261 cgacacacac ttcgcgaggc tcgtgcgccg gttcggctgg actgacgaga cccacccggt
     4321 caaggtggag cacgcggtcg gggcgttgtt ccccaagcgg gactggacga tgctcagcca
     4381 tcacctgatc tggcacggcc gccgccgttg tcacgcgcgc aacccggcct gtggcgcctg
     4441 cccgctcgac cggctgtgcc cgtcgtacgg ggaggggccg accgacccgg tgaaagccgc
     4501 caagctcgtc cgcacagagg gtcgcgcgtg atcgcgcgac accttcggta cggctcgcac
     4561 agcgcaacgc cggtacggct ggcggtggtg gccgccgccg gcgccgtcac ggcgtcgctg
     4621 ctggtctcgt gcagcagctc gacgccgcac gtcgatgcgt acacctacaa cccggagaag
     4681 gccgcggtcg acgtcgacac tgccgacctc cgccgacaga aggcgatcgc cggcatcccg
     4741 aactgtccgc ggtcgacgca gggcgcgacg cagcaggaca agggcttgcc gccgatcacc
     4801 ttgccgtgtc ttggtggggg acgtgacgtc gacctggccg gcctgaccgg accgccgacc
     4861 gtgctcaact tctgggcgca gacctgcgga ccgtgccggc aggagtcacc gctcttccag
     4921 cgactccatg acagtggtgc tgttcgtgtc gtcggtgtcg acttcctcga tccgctgccg
     4981 gaccgagcga tcgccttcgc caaggagctc ggtctcacct acccgcaggt tgccgacccc
     5041 gaaggtgcca cccgcgcccc gttgcacgtg tctggactcc ccatgacgat gttcgtggac
     5101 agccagggcg tggtgcgtca cgtcgaatac ggcgccgtcc ggtcggccgc ctctctcgct
     5161 gcactggtgt cgacgtacct cgccgtccac gtggatctcg ggtgagcacg tgatcgcgct
     5221 cccacccggc gcggacgacc gacggcgagg gcggaccgga gaccttcccc aatggttgca
     5281 gctgctgagt gatgtcgtcg gctccatccg tgtcgggcag ctcacgtcgt tcctcccgcc
     5341 acgcgatgcc gatccacgtc cggccgcggt gctcatgctg ttcggggagt cgtccggtga
     5401 gcccgacgtg ctgctgctcg aacgggcgca cacgatgcgc tcgcatgccg gccaggtcgc
     5461 gttccccggc ggggtgctgg atccggatga cgcggacgcg gtggctgcgg cgctgcgcga
     5521 ggcgcaggag gagaccggtc tcgacccgtc gggtgtggag gtcctcggca tactcccgag
     5581 cctctggcta ccaccgacaa acttcgcggt caccccggtc gtcggatggt ggcgcacccc
     5641 gacgccggtg gcggtggtcg accccgccga gacggcctcg gtgcacgccg tgcccatcgc
     5701 cgagctcgcc gaccctgcga accgggcaac agtccgccac ccctcgggtt acctcggccc
     5761 cgcgttcctg gtgcgtgacc tcgtggtttg gggcttcacc gctggcctgc tggcccggat
     5821 cttcggcatc gtggggtggg agcgtccgtg ggacgactcg cgcatcgtcg acctgcctgc
     5881 ggacctggtg tcgagctcac tgcgcgacct cgaacgtgcc gcggagtccg gcgcgctgcc
     5941 accgagccga ccgccaggat ccggaccgga gtccccccgg tgaacgcgct cgactggatc
     6001 ctgatcctgt tcgccgtgct gtacgccctg tcgggatacc agcaaggctt catcgtcgga
     6061 gccgcgtcga cgatcggcct gctggccggt ggcttcatcg gggtccaggt cacgccctgg
     6121 ctgctcgacg ggttcgaccc ggcgctgtcg gtctccgtcg ccgcactgct ggtcgtgctg
     6181 gcgtgcgcgt tcgtcggcca ggccatcggt gcgtacgtcg gcggggcgct gcggggtcgg
     6241 ctcacctggc agccggcacg ggtcctggac gcctgcagcg gagcggcgtt gagcgtggtc
     6301 gcgatgctgc tgatcgcgtg ggtgctgggg gtggccgcca gcggcgccga cctgcgcggg
     6361 ctcaacaacg aggtccggac gtcgtcggtg ctcgccggcg tcgacaaggc gctccccgga
     6421 gggtccgacc gggtgctgtc cgcgttcaac tccctggtgg actcctcgct gttcccgagg
     6481 tatctcgagc cgttcacgcc ggagcacatc aaggaggtac cgccgccgaa ccgcggtgtg
     6541 gtcgcgaagc ccggcgttcg ggcggcggag gacagcgtgg tcaagatcct gggcgccgcg
     6601 gactcctgt
//
LOCUS       DATBFZ010000365         3733 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_2222203, whole genome shotgun sequence.
ACCESSION   DATBFZ010000365 DATBFZ010000000
VERSION     DATBFZ010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 3733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3733
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2222203"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /locus_tag="VH419_11060"
     CDS             <1..513
                     /locus_tag="VH419_11060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY7044197.1"
                     /translation="THLIAGLLVAGLGIGMINVPLASTAVGVVRPERAGMASGVNSTA
                     RQVGIATGIAALGSIFSHHIASGISDRLSTTPMDSHSGQIADAVTGGQLGQVVTQVPP
                     GERGTIIQAATASFVDSLNHITMISAALAFATGVACLFLIRQRDFEAHHGGDYVAETE
                     DVPTGGAISH"
     gene            complement(539..1069)
                     /locus_tag="VH419_11065"
     CDS             complement(539..1069)
                     /locus_tag="VH419_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044198.1"
                     /translation="MYNVVVLAEQAMSAGDAAEVAALHDALEEPRHYHVLIPCENAAV
                     RVETALGALAASEVLAAPPVVTTDMDAAESQKQIDAHAETAVTVSTAALRTLGHEASG
                     EFSSDDPIDALERVSRKQDAREIIVMTRPHVVAEFLHLDWASKARRRLGVPVLHLLEH
                     ERLDHDAGAGEGVTGM"
     gene            complement(1168..2253)
                     /locus_tag="VH419_11070"
     CDS             complement(1168..2253)
                     /locus_tag="VH419_11070"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012920926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEY7044199.1"
                     /translation="MQLPVMPPLSPMLAKTAKGVPDPTKFEGGLLYEPKWDGFRCIVF
                     RDGDEVELGSRNERPLTRYFPEVVAAAKRSLPRRCVVDGEIVVAMDGRLEFERLLERI
                     HPADSRVQKLAVETPASYVAFDLLAIDDRSLMQTPMGERRAQLERALAESTAPIHLTP
                     ATDDPATAQEWFNSFEGAGLDGIVAKPNDGGYEPNGRVMIKVKHERTCDAVVAGYRLH
                     KKSTPEKQLLGSLLLGLYDDQGRLQHVGVSAAFAEARRAELIHELEPLLVDPHNHPWR
                     EWAEAEAHEKSRLPGAVSRWNAKKDLSWIPLDPIQVVEVGYDHMEGSRFRHTAQFKRW
                     RPDRTPESCTYAQLEEVVGYDLADILR"
     gene            complement(2261..3013)
                     /locus_tag="VH419_11075"
     CDS             complement(2261..3013)
                     /locus_tag="VH419_11075"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEY7044200.1"
                     /translation="MQSLFPTDPSEVTDEDLVELYAYPSESTWVRANFVSSVDGAAQG
                     IRGRSGELSPSADQKLFALLRSLADVILVGGQTARVEGYQPVLPSEVDGALRARLGLT
                     ATPAIAVVSRAMDFDPGLLAGGAAPTLVMTTAVAAEQHADVLAGATVIDAGRPGDPVH
                     VDAGRIIDELAARGYQRILCEGGPSLHYGLVVSGRLDELCLTINSQLAGGDPLRILTA
                     GPYFEPPIRLELRHLLTEDGDLFCRYTTERHR"
     gene            3069..>3733
                     /locus_tag="VH419_11080"
     CDS             3069..>3733
                     /locus_tag="VH419_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007077570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPK2 family polyphosphate kinase"
                     /protein_id="HEY7044201.1"
                     /translation="MAPSSVSVSDLLRVPPGRVGLAAIDPRSTPGFRGDKAAGRKALS
                     ALGDEVADLQERLYAESRGDVKRSVLLVLQGMDTSGKGGVMRHCVGLLDPQGVMITGF
                     KAPTEEERSHDFLWRIRRRLPAPGMIGIFDRSHYEDVLVARVHGLASGGELDRRYDVI
                     NDFELELVGNGTRVITCMLHISKREQGERLRARLDDPTKHWKFNPGDIDERARWDDYM
                     AAY"
BASE COUNT          585 a         1268 c         1277 g          603 t
ORIGIN      
        1 acccacctga tcgcgggctt gctggtggcg ggtctcggga tcggcatgat caacgtgccg
       61 ctggcgtcca ctgcggtcgg cgtcgtacga cccgagcgtg ccggcatggc atccggggtg
      121 aactcgaccg cccggcaggt cggcatcgcc accggtatcg cggcgctggg ctcgatcttc
      181 agccaccaca tcgcgagcgg gatcagcgac cggttgtcca cgacaccgat ggacagccac
      241 tccggtcaga tcgccgacgc cgtgaccggc gggcagctcg gacaggttgt cacccaggtc
      301 ccgccgggag aacgcggcac gatcatccag gcggcgacgg cgagcttcgt cgactcgctc
      361 aaccacatca cgatgatctc ggccgctctc gccttcgcca ccggtgtggc atgcctgttc
      421 ctcatccggc agcgtgactt cgaggcccac cacggcggcg actacgtcgc ggagaccgag
      481 gacgttccca ccggcggcgc catcagtcac tgaccgaccc accgaggggc ctcgttcgtc
      541 acatgccggt gacgccttcg cccgcacctg cgtcgtggtc gagacgctcg tgctcgagca
      601 gatgcaggac gggtacgccg aggcgccgac gcgccttgga cgcccagtcg aggtgcagga
      661 actcggccac gacgtgcgga cgggtcatca cgatgatctc ccgcgcgtcc tgcttcctcg
      721 aaacccgctc gagcgcgtcg atcgggtcgt ccgagctgaa ctcgccgctg gcctcgtggc
      781 cgagggtgcg caacgcggcc gtgctgacgg tcacggcggt ctctgcgtgc gcgtcgatct
      841 gcttctgcga ctcggcggcg tccatgtccg ttgtgacgac cgggggtgcc gccagtacct
      901 cgctggcggc cagcgcgccg agtgccgtct cgacgcggac agcagcgttc tcgcaaggga
      961 tcagcacgtg gtagtgacgg ggctcttcga gggcgtcgtg caaagccgcc acctcggcgg
     1021 cgtcaccggc gctcatggct tgttcggcca ggacgacgac gttgtacatg gcacgctcct
     1081 cacgcgacat tgcacctcca tgatgctccc cgttcgaccg agcgccaaag cctgacacac
     1141 acgagcatct gccggggagc gccggggtca gcgcaggatg tcggccaggt cgtaaccgac
     1201 gacctcttcg agctgtgcgt acgtgcagga ctccggtgtg cggtccggtc gccaccgctt
     1261 gaactgcgcg gtgtgccgga acctgctgcc ttccatgtgg tcgtatccga cctccaccac
     1321 ctgtatcggg tcgagcggga tccaggagag gtccttcttt gcgttccatc gcgaaacagc
     1381 gccgggaagc cgactcttct cgtgcgcctc ggcctcggcc cattctcgcc agggatggtt
     1441 gtgcggatcg acgaggagcg gttcgagctc gtggatcagc tcggcgcgtc gagcctcggc
     1501 gaacgccgcc gagacgccga cgtgctggag gcggccctgg tcgtcgtaca ggccgagcaa
     1561 caacgagcca agcaactgct tctccggcgt cgacttcttg tgcaggcggt agccggcgac
     1621 gacggcgtcg cacgtgcgtt cgtgcttcac cttgatcatg acccggccgt tcggttcgta
     1681 gccgccgtcg ttcggtttgg ccacgatgcc gtcgagtccg gcgccctcga agctgttgaa
     1741 ccactcctgg gccgtcgcgg ggtcatcggt cgccggtgtc aggtggatgg gggcggtcga
     1801 ctcggcgagc gcgcgctcga gctgtgctcg gcgttcgccc atcggcgtct gcatgagcga
     1861 acggtcgtcg atggcgagga ggtcgaaggc gacgtacgac gcgggggtct cgacggcgag
     1921 cttctgcacc cgcgagtcgg ccggatggat gcgctcgagc agtcgttcga actccagccg
     1981 cccgtccatc gccaccacga tctcgccgtc gaccacgcag cgacgaggca gcgatcgctt
     2041 tgccgccgcg accacttcgg gaaagtagcg ggtgagcgga cgctcgttgc ggctgccgag
     2101 ctcgacctcg tcaccgtcgc ggaagacgat gcaccggaag ccgtcccact tcggttcgta
     2161 gagcagccca ccctcgaact tggtgggatc gggcaccccc ttcgcggtct tggcgagcat
     2221 cggggagagc ggcggcatca ccgggagctg catgtcgaga tcacctgtgt ctttcggtcg
     2281 tgtagcggca gaacaggtcg ccgtcctcgg tcagcaggtg gcgcagctcg agccgtatcg
     2341 gcggctcgaa gtaggggccg gccgtcagga tgcgcagcgg gtcgccaccg gcgagctggg
     2401 agttgatcgt caggcagagc tcgtcgagcc tgccgctcac gacaaggccg tagtgcagag
     2461 acggaccgcc ctcgcacagg atccgctggt agccgcgggc ggccagctcg tcgatgatgc
     2521 ggcccgcgtc gacgtggacc ggatcacccg gccgaccggc gtcgatcacg gtcgcgccgg
     2581 ccaggacgtc cgcatgctgc tcggccgcga ccgccgtggt catgacgagc gtcggggccg
     2641 cgccgccggc aagcaggccg ggatcgaagt ccatcgcgcg cgacacgacc gcgatcgccg
     2701 gggtcgccgt caaccccaac cgcgcccgca gcgcgccgtc cacttcgctg ggaagcaccg
     2761 gctggtagcc ctcgacccgc gcggtctggc ccccgacgag aatcacgtcg gccagcgagc
     2821 gcagcagcgc gaacagcttc tggtcggcgc tcggcgacag ctcgccggac cggccgcgga
     2881 taccttgcgc ggcgccgtcg acactcgaca cgaagttggc acgaacccag gtcgactccg
     2941 acgggtacgc gtacagctcg acgaggtcct cgtcggtcac ttcgctcggg tctgtcggga
     3001 acagcgactg cacgcttcag atcctcccat cccggccgaa cagcgtcgga ggcaccagaa
     3061 tggccgccat ggctccctct tcggtttctg tgtccgacct gctccgggtt ccaccaggtc
     3121 gggtagggct cgccgccatc gatcctcggt cgacgccggg cttccggggc gacaaggctg
     3181 ccggcaggaa agcgctgtcc gccctcggtg acgaggtcgc agacctgcag gagcggttgt
     3241 acgccgagtc gcgcggcgac gtgaaacgca gtgtgctgct cgtgctccag ggcatggaca
     3301 cctcgggcaa gggaggagtc atgcgacact gcgtcggttt gctcgaccca caaggcgtca
     3361 tgatcacggg gttcaaggcg ccgacggagg aggagcggtc acacgacttc ctctggcgga
     3421 tcaggcggag gctgcccgct cccggcatga tcggcatctt cgatcgcagc cactacgagg
     3481 acgtgctcgt ggcccgggtg cacggccttg cgtcgggcgg cgagctcgat cgacggtacg
     3541 acgtgatcaa cgacttcgag ctcgagctgg tcggcaacgg gacccgcgtc atcacgtgca
     3601 tgctccacat ctccaagcga gaacagggtg agcgactacg ggccaggctc gacgacccga
     3661 cgaagcactg gaagttcaac cctggcgaca tcgacgagcg ggcgcgctgg gacgactaca
     3721 tggcggcata cga
//
LOCUS       DATBFZ010000366         1853 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_3733696, whole genome shotgun sequence.
ACCESSION   DATBFZ010000366 DATBFZ010000000
VERSION     DATBFZ010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 1853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1853
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3733696"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            442..>1853
                     /locus_tag="VH419_11085"
     CDS             442..>1853
                     /locus_tag="VH419_11085"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY7044202.1"
                     /translation="MVGANAAGTGTVTRSDTVRRYAAASGFTGFEVLTVENAFYRCER
                     LTPSTADSHQTPSTARPLSRQPRQVRSSSPAVADGTDKTTFAVVIAPTNCMPIGTLLT
                     ADRRPPRSRGARLAGVCRRGADLTEGDPVEFGMCVTTKLTDVDFARRCEDLGYDMVWV
                     PDSQMIWGDCYAYMAMAAERTRRIRLGTGVAVAGTRIAPVIAHSIASVNTLAPDRVSL
                     GIGSGNSAYRFMNLKPLPARKYAEEVRVIRALLDGEEVEYTFRGVTAPIRLLMADRGY
                     VDLEHRIPIIVSGFGPKSQSVAGQLGDGLFVSLPVEERSIERARQTVAHAAEEVGRSI
                     DFGSYPVVNLVNVIMLDPGEDILSDRVVAEHGPFIVSGMHYLFDQVRQYAREPPRRLL
                     DVWDKYTALIARVPEHVRHLRIHEGHCTYLLDEERALLTADLVRATSVVGTPEECREQ
                     IRRLERAGVTHIVGLPTPGT"
BASE COUNT          301 a          623 c          632 g          297 t
ORIGIN      
        1 ggacgctggg tgccaggccg ctccgttcga cgaggcggcg gtggacgaga cctgtgacga
       61 tgccggagag tccgacgaac aggaacgccc cgtcgatcca atgggcgctg tgcaggacgc
      121 ggccgatgac cgaccgggtg atggaagtcg tcgacatggc tggtgatcat cgacacgagg
      181 gcgagccctc gtaggacgtc gatcgcggga tagcgcgatc gagcccgctc cgcccgccgg
      241 cccgccattg cgagcacggt agcgagcgcg tcgaggccag gagacgtgct ctgttgtctg
      301 atccgaggat cacggggttt ccccgattcg cctgacgatc gtcggcccga gacttccgat
      361 ccggcgtcgc ggtgctcgac gtccgcgggt cgtgctctct cgttgacgcg cggacggctg
      421 tgccgcgacg ccaccatcgg gatggtcggt gccaacgcgg ccggtacggg aacggtgacg
      481 cgctcggaca cggtccggcg ctacgccgcg gcgtccggct tcacgggctt cgaggtactg
      541 acggtcgaga acgccttcta ccggtgcgag cgcctcacac catcaaccgc tgactcccac
      601 cagacaccgt ccacggctcg gccgctctcc cgacaaccaa gacaggttcg gtcaagcagc
      661 ccggctgtcg ctgacggcac cgacaagacg accttcgccg tggtcatcgc cccgacgaac
      721 tgcatgccta tcggtacgct gctgaccgcg gaccgtcgtc cgccacgcag tcggggcgcc
      781 cgactggccg gtgtttgtcg acgtggagct gatctgacgg aaggggaccc cgtggagttc
      841 ggcatgtgtg tcaccaccaa gttgaccgac gtcgacttcg ctcgccgctg cgaggacctc
      901 gggtacgaca tggtgtgggt gcctgacagt cagatgatct ggggtgactg ctacgcctac
      961 atggcgatgg cggcagagcg cacccgtcgc atccgcctcg gcacgggagt ggcggtggct
     1021 gggacgcgca tcgcgccggt catcgctcac agcatcgcga gcgtcaacac gctcgctccc
     1081 gacagggtgt cgctgggcat cggttcgggc aacagcgcct accgcttcat gaacctgaag
     1141 ccgctgccgg ccaggaagta cgccgaggag gtccgcgtca tccgggccct gctcgacggt
     1201 gaggaggtcg agtacacgtt ccgcggagtg accgccccga tccggctcct gatggcggat
     1261 cgcgggtacg tcgacctcga acatcggatt ccgatcatcg tgtcggggtt cggcccgaag
     1321 tctcaatcgg tcgccgggca gctcggagac gggctgttcg tgtcgctgcc ggtcgaggag
     1381 aggtcgatcg aacgggcccg ccagaccgtc gcgcacgccg ccgaggaggt cggcaggtcg
     1441 atcgacttcg ggtcgtaccc cgtcgtcaac ctcgtgaacg tcatcatgct cgacccgggt
     1501 gaggacatcc tgtccgacag ggtcgtcgcc gaacacggcc cgttcatcgt cagcggcatg
     1561 cactacctgt tcgaccaggt acgccagtac gcccgtgagc cgccgcgccg tctcctcgac
     1621 gtgtgggaca agtacacggc gctgatcgct cgagtgcccg agcacgtccg ccaccttcgc
     1681 atccacgagg gccactgcac gtacctgctc gacgaggagc gcgccctgtt gaccgccgat
     1741 ctcgtgcggg ccaccagcgt ggtggggaca ccggaggagt gccgcgaaca gatccggcgg
     1801 ctcgaaaggg ccggggtcac ccacatcgtc gggctcccga ctccgggcac gca
//
LOCUS       DATBFZ010000367         2835 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_6036673, whole genome shotgun sequence.
ACCESSION   DATBFZ010000367 DATBFZ010000000
VERSION     DATBFZ010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2835
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6036673"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..504
                     /locus_tag="VH419_11090"
     CDS             <1..504
                     /locus_tag="VH419_11090"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020577676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEY7044203.1"
                     /translation="VVRKALAWSVVVIESDECDRLGMHVANVEALRRAVALLDVAPEY
                     VLTDGFPVDGLGVPGLAVWKGDRVAACISAASVLAKVTRDRIMVELDREHPAYDFKTH
                     KGYITDVHAAALTEHGPCSQHRMRFVNVRRAARLEFPHGAELRWGNEENADSHAGPDT
                     VDAPSLG"
     gene            538..846
                     /locus_tag="VH419_11095"
     CDS             538..846
                     /locus_tag="VH419_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2469 domain-containing protein"
                     /protein_id="HEY7044204.1"
                     /translation="MSAEDLEKYETEMELTLYREYRDVVGIFKYVVETDRRFYLCNQV
                     DVKARTESGDVFFEVSMTDAWVWDMYRPARFAKNVKVLTFKDVNVEELSEQEIDPPKV
                     "
     gene            1096..1746
                     /locus_tag="VH419_11100"
     CDS             1096..1746
                     /locus_tag="VH419_11100"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE family protein"
                     /protein_id="HEY7044205.1"
                     /translation="MAVEAALITPVLVLLIFGIIEFAFVMRDYGVVSSDVRSGARIAS
                     TGAGLGPGTCDTSYTNPPPCTPAKSPALAQLAADAIQREGSAMPANQINYILVYKANT
                     QGYPGADGNTTMPSNCSGFSNCVRFTWQQKANGGAGAFRYTDGAWSSSTISACFPGTG
                     SATLDRVGVFMNAKHNMMTGLFGSSITITDHAVFDFEPLPSATCNGTGNPNTGGHS"
     gene            1743..>2835
                     /locus_tag="VH419_11105"
     CDS             1743..>2835
                     /locus_tag="VH419_11105"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HEY7044206.1"
                     /translation="MSRLRRRWQRRHLDQRGYVAILVATLLPTVFLVCGAFAVDTGNW
                     YAQEKLMQKAADAAALAGVPYLPNDLANATTRAKAVAARNGYDDAKADIVVTVSVGDR
                     PTQLKVTISAVVKNTFARTIGIPTTTLERTATADYQGPQPMGSPCNTFGNEPSSGGGT
                     NSVTPTGTAQAPPAVRYPNCTSNPQYWPTIEGPETDKEQGDRYQTLVCKTGSQNCASN
                     KNQEYQNNGYILTIKVQPGAVNTPVTMQLYDPAYVGDDQFCSDNPTSGFSANMNPYVG
                     ADAASRYGRTGTSSTGAPYCPGDDQPGSTSSSLMTTSFAVRQQTDTQDPMKGAVISGC
                     VKQYTGQTAVPTATSLTAKKANGSANPQYN"
BASE COUNT          506 a         1003 c          932 g          394 t
ORIGIN      
        1 gtggtgagga aggcgctcgc gtggtccgtc gtcgtgatcg agtccgacga gtgcgaccgg
       61 ctcggcatgc acgtcgcgaa cgtcgaggcc ctgcggcgcg cggtcgcgct gctggacgtc
      121 gcgccggagt acgtcctcac cgacggcttc ccggtcgacg gactgggcgt ccccgggctc
      181 gcggtctgga agggcgaccg ggtcgccgcg tgcatcagcg ccgcgtccgt cctcgccaag
      241 gtgacccgcg accggatcat ggtcgagctc gaccgcgagc acccggcgta cgacttcaag
      301 acgcacaagg gctacatcac cgacgtgcac gccgccgccc tgaccgagca cggcccttgc
      361 tcgcagcacc ggatgcgctt cgtcaacgta cggcgcgcgg cgagactcga gtttccgcac
      421 ggagcggagc tccggtgggg aaacgaggag aacgcagact cacacgccgg tccggacaca
      481 gtcgatgcgc cgtcccttgg ctagggtcgg cccgaggatg acacgagggg agtcccgatg
      541 agtgccgagg atctcgagaa gtacgagacc gagatggagc tcaccctcta ccgggagtac
      601 cgcgacgtcg tcgggatctt caagtacgtc gtcgagaccg accgccggtt ctacctctgc
      661 aaccaggtcg acgtgaaggc gcgcacggag tccggcgacg tgttcttcga ggtgtcgatg
      721 accgatgcgt gggtctggga catgtaccgc cccgcccgct tcgccaagaa cgtcaaggtg
      781 ctcacgttca aggacgtgaa cgtcgaggag ctcagcgagc aggagatcga cccgccgaag
      841 gtctgagcgg gcctgccggg cacggcggag gccccgaaat accccaggct ggtctggacc
      901 aggtcggcca ggggtaaaac cgcggtcaaa tttcctcgtt ccgcttcggg ccgcgggtga
      961 agactcctac gctttcgccc ggagatcggt ggatcggggg acccgccgac ccctgacttt
     1021 gatgggagac ggccggtgag acgtgccttc aggtggtcgc gtaggtggtc gcgccgacgc
     1081 ggcgaaggtg gggccgtggc ggtcgaggct gccctgatca cgccggtgct cgtgctgctg
     1141 atcttcggca tcatcgagtt cgccttcgtg atgcgcgact acggcgtggt gtcgtccgac
     1201 gtccgctccg gcgcccgcat cgcgtcgacc ggtgccggtc tcggccccgg cacgtgcgac
     1261 acgagctaca ccaacccgcc gccgtgcacg cccgcgaagt cgccggccct ggcccagctg
     1321 gccgccgacg ccatccagcg tgagggctcg gcgatgccgg ccaaccagat caactacatc
     1381 ctggtgtaca aggccaacac ccagggctac ccgggcgcgg acggcaacac gaccatgccg
     1441 tcgaactgct cgggcttctc caactgcgtc cgcttcacct ggcagcagaa ggccaacggc
     1501 ggggccggtg cgttccggta caccgacggc gcctggagct cctcgacgat cagcgcctgc
     1561 ttcccgggca ccggcagcgc cacgctggac cgcgtcggtg tgttcatgaa cgcgaagcac
     1621 aacatgatga ccggcctctt cggctcgtcg atcaccatca ccgaccacgc cgtcttcgac
     1681 ttcgaacccc tcccctcggc cacctgcaac ggcaccggca acccgaacac cggagggcat
     1741 tcgtgagtcg tctccgccgg cgctggcagc gccgtcatct cgaccagcgg gggtacgtcg
     1801 cgatcctggt cgcgaccctg ctcccgaccg tcttcctcgt ctgcggggcc ttcgccgtcg
     1861 acaccggcaa ctggtacgcc caggagaagc tcatgcagaa ggccgcggac gcggccgccc
     1921 tcgccggggt cccgtacctg ccgaacgacc tggccaacgc cacgacgcgc gccaaggccg
     1981 tggccgcgcg caacgggtac gacgacgcga aggcggacat cgtggtcacc gtgtcggtcg
     2041 gcgaccggcc gacgcagctc aaggtgacga tcagcgcggt cgtcaagaac acgttcgcgc
     2101 gcacgatcgg gatcccgacg accaccctgg agcggaccgc caccgccgac taccagggac
     2161 cgcagccgat gggcagtccc tgcaacacgt tcggcaacga gccctcctcg ggcgggggga
     2221 ccaactcggt gacgccgacc gggaccgcgc aggcgccacc cgccgtccgt taccccaact
     2281 gcacctcgaa cccgcagtac tggcccacga tcgaaggtcc cgagaccgac aaggagcagg
     2341 gagaccgcta ccagacgctc gtctgcaaga ccgggtccca gaactgtgcg agcaacaaga
     2401 accaggagta ccagaacaac ggctacatcc tgaccatcaa ggtccagccg ggtgcggtga
     2461 acacaccggt cacgatgcag ctctacgacc cggcgtacgt cggggacgac cagttctgca
     2521 gcgacaaccc gaccagtggg ttcagcgcga acatgaaccc gtacgtcggc gcggacgcgg
     2581 cgtcgcggta cggccgcacg gggacctcgt cgaccggcgc gccgtactgc cccggtgacg
     2641 accagccggg cagcacctcc tcgagcctga tgacgacgtc gttcgccgtc cgccagcaga
     2701 ccgacaccca ggacccgatg aagggggcgg tcatcagcgg ttgcgtcaag cagtacaccg
     2761 gtcagaccgc cgtgccgacc gcgacctccc tcaccgctaa gaaggcgaac ggcagcgcga
     2821 acccccagta caacg
//
LOCUS       DATBFZ010000368         2515 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_910908, whole genome shotgun sequence.
ACCESSION   DATBFZ010000368 DATBFZ010000000
VERSION     DATBFZ010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2515
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_910908"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="VH419_11110"
     CDS             complement(<1..238)
                     /locus_tag="VH419_11110"
                     /inference="COORDINATES: protein motif:HMM:NF020905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1989 domain-containing protein"
                     /protein_id="HEY7044207.1"
                     /translation="MDEAGTEWEVDEVRPGMGGARWVPTGGLVRVTDVEGGQSGDVFF
                     VDAGDVTDGLSNDRSFDCNGTISLTVGATLFSSSS"
     gene            457..960
                     /locus_tag="VH419_11115"
     CDS             457..960
                     /locus_tag="VH419_11115"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEY7044208.1"
                     /translation="MVAAPKGGDGRIGGPVANGSVVAVPPRLELKQLIRAVPLFAGLL
                     PSEVDRIALVMRPRDVGAGVVICAEGEPGHEFFVLARGEAGIERGDETIAKLHVGDHF
                     GELALLDRGPRSATVRALGDCRLYVLEDAAFAAVLNEVPALAQKLLATLAGRLREAET
                     PPQGEKR"
     gene            1363..2466
                     /locus_tag="VH419_11120"
     CDS             1363..2466
                     /locus_tag="VH419_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006901171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEY7044209.1"
                     /translation="MAMTTMTHPRDVAVRVFGGVDTHKDVHVAAALDELGRLLGTGSF
                     ATTTDGYRQLWRWLRSWGEVVTVGVEGTGSWGAGLARHLTAAGVDVREVMRPNRQHRR
                     RYGKSDQADAIGAARAVLAGEAVGTPKAGTGQVEAIRLLRIARRSAMKARTQAGNQIH
                     AVIDTAPEQLRRRFIARATDDIVAETAMFRRRHAPTTPLEAARLSLRTLARRWQYLDD
                     EMAALDVQLDELTARVAPTLRAINGVGPQVATAVLAAAGDNPERLHSSASFAALCGVS
                     PIDTSSGRQQHHRLNLYGDRHANWALHVIIISRLRWHPPTQAYMARRLAEGRTQKMII
                     RCLKRHVVREVYQAITTDLGIRNDLEQQQRTAA"
BASE COUNT          415 a          909 c          821 g          370 t
ORIGIN      
        1 gactgctcga cgagaacagt gtcgctccga cggtcagcga gatcgtcccg ttgcaatcga
       61 acgacctgtc attgctcagc ccgtcggtca catcgccggc gtcgacgaag aagacgtcgc
      121 ccgactgacc accctccacg tcggtcacgc gcaccagccc acccgtgggc acccagcgtg
      181 caccgcccat gcccggtcgg acctcgtcca cctcccactc agtcccagcc tcgtccacca
      241 tcccagattg cccatcgagc gccagatacc cgcgtccgat atcgcgtccg gatagcaccg
      301 gccgtcgcgt cctcgaccgc cggatcccag ggttggttgc tcgacgcgcc tggcagcatg
      361 cggagcccgt gtcagcgatg agcgattgga cctcggtgca cggtcgtaac acatgcaggg
      421 tgttgaacgc gccgtttcgg tgagacgtcg tcggtgatgg ttgcagcgcc caagggcggc
      481 gacggccgga tcggcggccc ggtggcgaac ggtagcgtcg tggccgtgcc gccgcgattg
      541 gagctgaagc agctcatccg ggcagtgccg ctgttcgccg gtctccttcc ctccgaggtc
      601 gaccgcatcg cgctggtgat gcgccccagg gacgtcggag ccggcgtggt gatctgcgcc
      661 gaaggcgagc ccgggcatga gttcttcgtg ctcgcccgcg gcgaggcggg gatcgagcgc
      721 ggggacgaga cgattgcgaa gctgcacgtc ggcgaccact tcggtgagct ggcgttgctt
      781 gatcgcggtc ctcgctccgc caccgttcga gcgctcggcg actgccggct ctacgtgctc
      841 gaggacgccg cgttcgccgc cgtcctcaac gaggtcccgg cgctggcgca gaagctgctc
      901 gccactctcg ccggtcgcct gcgcgaggcc gagacgcctc cccaaggcga aaagcgctga
      961 gcaccgtccc ccgggacgcg cccgaagcca cggcgagacg ccaccgcgtc ggtcctgtca
     1021 gtccgggcca cgccgagcac ctcagtcgga ggaccctcgg agcttggccc gaagcgacgg
     1081 aacgccgccc gcgtatccgg atagggccgc cggataaccc agggctccgc gtccggtacc
     1141 gccaatgaga atggtggtgg cgctccgctc gtcgggtctg actctgctac cgcctgactc
     1201 ctctggggga tgtcctggtg tccgaactgt cgactcggtg agcggagcgc caccggcaca
     1261 accagacggt cgaacgtgac ctgaaagaag ctcgtgcgag gagcctcgtc attggcctgt
     1321 ccgtgaccgg ctgtgcgtgc catccgcaac caggacgaag ggatggcgat gaccacgatg
     1381 acacatccac gggatgtcgc ggttcgcgtg ttcggcgggg tcgacacgca taaggacgtt
     1441 catgtcgcag ccgccctcga tgagctcggc cggctgctcg gcacgggatc gttcgcgacg
     1501 acgaccgacg gctaccggca gctgtggagg tggctgcgca gctggggcga ggtcgtcacc
     1561 gtcggggtgg aggggaccgg ctcgtggggt gcgggcctgg cccggcatct caccgcggcc
     1621 ggtgtcgacg tgcgtgaggt gatgcgcccg aaccgtcagc accgtcgtcg ctacggcaag
     1681 tccgaccaag ccgacgcgat cggcgcagcc cgagcagtgc tagcaggtga agcagtcggc
     1741 accccgaagg cgggcactgg ccaggtcgaa gcgatccgtc tgctgcggat cgcccgacgg
     1801 agtgcgatga aggcccgcac gcaggccggc aatcagatcc acgccgtcat cgacaccgcc
     1861 cccgagcagc tccgtcgccg cttcatcgca cgggccaccg acgacatcgt cgccgagacg
     1921 gcgatgttcc gccgccgtca cgccccgacc acgccgctcg aggcagcccg gctcagcctg
     1981 cgcaccctcg ctcggcgctg gcagtacctc gacgacgaga tggccgccct cgacgtccag
     2041 ctcgacgagc tcaccgcgcg agtcgctccg acactgcgag cgatcaacgg tgtcggtccc
     2101 caagtcgcca ccgccgtgct cgcggccgcc ggcgacaacc ccgagcggct gcactcctct
     2161 gcgtcgttcg ccgcgctgtg cggagtgtca ccaatcgaca cctcgtccgg ccgccaacag
     2221 caccaccggc tcaacctcta cggggaccgt cacgccaact gggcgctgca cgtgatcatc
     2281 atcagccgtc tgcgctggca cccgccgacc caggcctaca tggcccggcg actggccgag
     2341 ggacgcaccc aaaagatgat catccgctgc ctcaaacgac acgtcgtgcg agaggtctac
     2401 caagccatca ccaccgacct cggcatccgc aacgacctcg aacagcagca acgcaccgcc
     2461 gcttgacatc caaagaagct ccgggaagcg cgtacaggcg gtcgtcgcct cgacg
//
LOCUS       DATBFZ010000369         4215 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_3206243, whole genome shotgun sequence.
ACCESSION   DATBFZ010000369 DATBFZ010000000
VERSION     DATBFZ010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 4215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4215
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3206243"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="VH419_11125"
     CDS             complement(<1..276)
                     /locus_tag="VH419_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013861535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HEY7044210.1"
                     /translation="MGLIGGMARTAVVAGTATAVSNRVSRRQAERWSAQEQPQYAQPQ
                     YAQPAYAPPPEPAPQAAPPEDDTIARLEKLADLKAAGALSDEEFAAAK"
     gene            complement(306..746)
                     /locus_tag="VH419_11130"
     CDS             complement(306..746)
                     /locus_tag="VH419_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017934495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6325 family protein"
                     /protein_id="HEY7044211.1"
                     /translation="MTELDAGPIDYLAVEFPQAKVTGEGLQALLDLVESGTIRILDLR
                     IVMRDADGTFKGAAIADLDHDGTLDLAVFEGVESGLLDDDDVSEAASLIDPGNAVGLL
                     VYENTWAEPFVTAMRKAGAEVIASGRIPADEVNAALDRLESDES"
     gene            complement(785..2296)
                     /locus_tag="VH419_11135"
     CDS             complement(785..2296)
                     /locus_tag="VH419_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017934488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HEY7044212.1"
                     /translation="MSDETTSQAAPAEPAKKKRRFALPSAYTILFALIVLAAIATWFV
                     PAGTYKLDKDGAPIPGTYHEVDAHPSRVVVDSLTAPINGLYGIENAKGGISYYNSGYL
                     FGAIDVALFILVIGGFLGVTMQTGAIQAGISKLVQKLRGRERWMIPILMSVFALGGTT
                     YGMAEESLAFYALVITVLIAAGYDALTGAAVVLLGCGIGTMGSTINPFATGIASGFAD
                     VSISDGILGRLVILLAGLAVGIWFVLRYADHVKADPSRSMVYDMKAANEAHFSATEDT
                     EADVVMTGKQKVILGLFGLAFVVMMYGVIPWSDLGIGLPTWWWWFPEMTASFLLFSII
                     IGVVGGMSEGVFTDTFVDGARDLLGVALIIGIARGITVIMNNGQITDTVLHWAEQALG
                     DVGQSGFAIVMYVLFLPLSFLIPSSSGLATVAMPIMAPLAGFVDVPKELVVTAYQSAN
                     GLMNLFIPTSAVVMGGLAIARVPYGSYLRWVWPLLAMLAGLTVVVLGVGAAVT"
     gene            complement(2302..3786)
                     /locus_tag="VH419_11140"
     CDS             complement(2302..3786)
                     /locus_tag="VH419_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017934489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HEY7044213.1"
                     /translation="MLDAVLPVAVLILLIVLTISLFGIDATNGPLQVALLLSAAFASL
                     IAFKNGYTVAAVADAAVGGVTSAVGAIFILLAVGALMGTWNMAGTIPTIVHYGIGILS
                     PTWFYFASAAICALVGVVTGSSWTTAGTLGVAFVGMASVLGLSEAAAAGSVICGAYFG
                     DKMTPLSETTILVPRLVGGITVGQHIRNMLWTAAPALGISLVIFFILGLTANPSGEAN
                     TDAAQAALEAEFNISPVNLLPLVLLIVFSFRRVPPFLAILGSALFAGILAPFTQWSMV
                     QQFVNEPGLGSVATGIKAIYASMATGFVSQSGNEKIDALFSRGGMASMLTTIWLVMGA
                     LSFAAVIEHAGFLDRLLQPIVARTKSRGSMIGAVNTTGVGLNVIAGDQYVAVVLPSRM
                     FRQEFARRGLAPQALSRAVEDSGTVTSVLVPWNTCGAYISGVLGVSTASYFPFCFFNI
                     LSPILDVVYGFIGFKVPTVPSGPEAEAAQVVADESAVDPDRRVR"
     gene            complement(3890..4042)
                     /locus_tag="VH419_11145"
     CDS             complement(3890..4042)
                     /locus_tag="VH419_11145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044214.1"
                     /translation="MGLGLGRIVLVMFIIAVLVALMSYARGAVHHHGDDIGSMSSIHA
                     AADLRG"
BASE COUNT          755 a         1404 c         1379 g          677 t
ORIGIN      
        1 cttggcggcg gcgaactcct cgtcggagag ggcacctgcg gccttcaggt ccgcgagctt
       61 ctccagtcgc gcgatcgtgt cgtcctctgg cggtgccgcc tgcggagccg gctcgggcgg
      121 tggcgcgtac gccggctggg cgtactgcgg ttgggcgtac tgcggctgct cctgcgcgga
      181 ccagcgctcc gcctggcgtc gtgacacccg gttcgatacc gctgtcgccg tgccggcgac
      241 gaccgctgtc cgggccatac ctccgatgag tcccatcgtt tgttccttcc tcccgctcag
      301 atctgctacg actcgtcgct ttcgaggcgg tccagagctg cgttcacctc gtcggccgga
      361 atgcggccgc tggcgatgac ctccgccccg gccttccgca tcgcggtgac gaacggctcc
      421 gcccaggtgt tctcgtagac gagcaacccg acagcgttgc ccgggtcgat cagcgacgct
      481 gcctcgctca cgtcgtcatc atccagcagt cccgactcga ccccttcgaa caccgccaga
      541 tccagcgtcc cgtcgtggtc gaggtctgcg atggcggcgc ccttgaacgt gccgtccgcg
      601 tcacgcatca cgatccgcag gtccagaatg cggatcgtgc cgctctccac gaggtcgagc
      661 agggcctgca gtccctcccc ggtgaccttg gcctgcggga actccaccgc gaggtagtcg
      721 atggggcctg cgtcgagctc agtcatgtct tgtctccact tccatggtca ggggtttctt
      781 tcacctaggt aacagccgct cccacaccga gtaccacaac agtcaagcct gccagcatgg
      841 ccagcagtgg ccacacccag cgaagatagc tgccgtacgg caccctggcg atggccagtc
      901 cacccatgac gacggcggac gtcgggatga acaggttcat gagtccgttc gcgctctggt
      961 acgccgtcac cacgagctcc ttgggcacgt cgacgaagcc ggccagcggc gccatgatcg
     1021 gcatcgccac ggtcgcgagt ccggacgacg acgggatcag gaaggacagc ggcaggaaca
     1081 ggacgtacat cacgatcgca aagccggact ggccgacatc tcccagggct tgctcggccc
     1141 agtggagcac ggtgtcggtg atctgcccgt tgttcatgat cacggtgatg ccacgggcga
     1201 taccgatgat cagcgcgacg ccgagcagat cccgtgcccc gtcgacgaac gtgtcggtga
     1261 acactccctc gctcatgcca cccaccacgc cgatgatgat gctgaacagg aggaacgacg
     1321 cggtcatctc cgggaaccac caccaccagg tggggagccc gatcccgagg tccgaccacg
     1381 ggatgacgcc gtacatcatg accacaaagg cgaggccgaa cagcccgagg atgaccttct
     1441 gcttgccggt catgaccacg tcggcctcgg tgtcctcggt cgcgctgaag tgggcctcgt
     1501 tcgcggcctt catgtcgtag accatcgacc gtgacgggtc cgccttgacg tggtcggcgt
     1561 acctcagcac gaaccagatc ccgaccgcca ggccggcgag aaggatcacc aggcggccga
     1621 ggatgccatc gctgatcgag acgtcggcaa aaccggacgc aatgccggtc gcgaacgggt
     1681 tgatcgtcga gcccatcgtc ccgatcccgc atcccagcag cacgacggcc gctccggtca
     1741 gcgcgtcgta tcccgccgcg atcagcacag tgatgaccag cgcgtagaac gccaggctct
     1801 cctccgccat gccatacgtc gtgccgccga gggcgaagac cgacatgagg atcgggatca
     1861 tccaccgctc gcggccacgc agcttctgca cgagcttgct gatgccggcc tggatcgctc
     1921 cggtctgcat tgtcacgccc aagaacccgc cgatgacgag gatgaacagc gccacgtcga
     1981 tcgcaccgaa caggtagccc gagttgtagt agctgatgcc gcctttggcg ttctcgatgc
     2041 cgtacagacc gttgatgggc gctgtgagcg agtccaccac gacgcgagac ggatgcgcgt
     2101 ccacctcgtg gtacgtgccc ggaatcggtg cgccgtcctt gtcgagcttg tatgtaccgg
     2161 cgggcacgaa ccaggtcgcg atggcggcga gcacgatgag cgcgaacagg atcgtgtacg
     2221 ccgacggaag cgcaaaccga cgcttcttct tggccggctc cgccggcgcg gcttgcgaag
     2281 ttgtctcgtc gctcatggaa atcacctcac ccgccggtcg gggtcgacgg ccgactcgtc
     2341 ggccacgacc tgcgcggctt ctgcttccgg cccgctcggg accgtgggga ccttgaatcc
     2401 gatgaacccg tacaccacgt cgaggatcgg gctcagaatg ttgaagaaac agaacgggaa
     2461 gtaggacgcc gtggacacgc cgaggacccc tgagatgtac gcgccacagg tgttccaggg
     2521 caccaggaca gacgtgacgg tgccggagtc ctcgaccgcc cgagacaggg cttgcggtgc
     2581 cagtccccgt cgtgcgaact cctggcggaa catccggctc ggcagcacga cggcgacgta
     2641 ctggtcaccg gcgatgacgt tgagaccgac gcccgtcgtg ttgacggcgc cgatcatgct
     2701 tccgcgcgac ttcgtgcggg cgacgatcgg ctggagcagc cggtcgagga agcccgcgtg
     2761 ttcgatgacc gcggcgaagg agagcgcacc catcacgagc cagatggtgg tcagcatgct
     2821 ggccatgcct ccgcgggaga agagcgcgtc gatcttctcg ttgcccgact ggctgacgaa
     2881 cccggtcgcc atcgacgcgt agatcgcctt gatgcccgtg gcgacggaac cgagacccgg
     2941 ctcgttgacg aactgctgca ccatcgacca ctgggtgaac ggcgcgagga tgccggcgaa
     3001 cagtgccgac ccgaggatcg ccaggaacgg cgggactcgg cgaaacgaga acacgatcag
     3061 caggaccagc ggcagcaggt tgaccggcga gatgttgaac tcggcttcga gggccgcttg
     3121 tgcggcgtcg gtgttggcct cgccgctcgg gttcgcggtg aggccgagga tgaagaagat
     3181 caccaggctg atcccgagcg cgggagcagc cgtccagagc atgttgcgga tgtgctggcc
     3241 gacggtgatc ccgcccacga gtcgcggaac caggatggtc gtctcggaca gcggcgtcat
     3301 cttgtcgccg aagtaggcac cgcagatgac cgaaccggcg gcggccgcct ccgacagacc
     3361 gaggacgctg gccatcccga cgaaggcgac cccgagtgtt cccgcggtgg tccaggaact
     3421 gcccgtcacc acgccgacca gggcacagat cgcggcgctc gcgaagtaga accacgtcgg
     3481 actcaggatg ccgatcccgt agtggacgat cgtggggatg gtgcccgcca tgttccaggt
     3541 acccatcaag gcaccgaccg cgagcaggat gaagatggca ccgaccgccg acgtcacgcc
     3601 gccgaccgcc gcatctgcaa cggccgccac ggtgtacccg ttcttgaagg cgatcaggct
     3661 ggcgaacgcc gcgctgagca acagcgccac ctggagcggc ccgttcgtcg cgtcgatccc
     3721 gaacagcgag atggtgagga cgatgagcag aatcagcacg gcgacgggca ggacggcgtc
     3781 gagcatggtc ggctctcgcg gtccatcggc acgaggcagt gtcgagtgag tcggctgcgt
     3841 cgacccggtg ctttcggggt caaccggctc cggaggctca gccatgggct cagccccgga
     3901 ggtccgccgc agcgtggatg ctgctcatgg aaccgatgtc gtcaccgtgg tggtggacag
     3961 cacctcgtgc gtacgacatc agggcaacca gaacggcgat gatgaacatc accaggacga
     4021 tgcgccccag tcctagcccc atctcgcacc tccttcagcc tgttggtgag actgcgcgct
     4081 cggggttgac cgcggctgcg ggttcatagg ttgccggccg ttgggctcgt cgcacccgaa
     4141 tccacttgtc ggcctcgacc acgaccggca ggacgaggga aaggccgagg caggccagcc
     4201 attcgtggtt ggtca
//
LOCUS       DATBFZ010000370        10825 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_4723479, whole genome shotgun sequence.
ACCESSION   DATBFZ010000370 DATBFZ010000000
VERSION     DATBFZ010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 10825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10825
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4723479"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..2246)
                     /locus_tag="VH419_11150"
     CDS             complement(54..2246)
                     /locus_tag="VH419_11150"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEY7044215.1"
                     /translation="MTGSDPKSVETVIDNGAHGKRTIRFETGQLARQAAGAAVAYIDE
                     ETMLLSATTAGKTPKEHFDFFPLTVDVEERMYAAGRIPGSFFRREGRPSEDAILTCRL
                     IDRPLRPSFVSGLRNEVQVVITVMALHPDTAYDVLAINAASMSTQLSGLPFSGPIGAT
                     RVALIDGDWVAFPTHTQLEKAVFDMVVAGRVTETGDVAIMMVEAESTEATWEKVQGGA
                     QAPTEEVVAQGLESSKAVIKQLVEAQQQLADVAAKPVTEFPRFLDYQDDVFEAVEGAA
                     SGELAQALTIADKLDRESKLDEIKASVLEKQGELFEGREKELGAAFRSLTKKLVRERV
                     LRDRVRIDGRGLTDIRTLSAEVGVVPRVHGSALFERGETQILGITTLNMLSLEQKLDT
                     LSPETTRRYMHNYNFPPYSTGETGRVGAPKRREIGHGALAERARIPVLPAREEFPYAI
                     RQVSEALGSNGSTSMGSVCASTMALLSAGVPLKAAVAGIAMGLISDEVEGKTQYVALT
                     DILGAEDAFGDMDFKVAGTREFVTALQLDTKLDGIPSDVLAAALQQAHEARLTILDVM
                     EEAIAGPAEMSDHAPRVTTIKIPVDKIGAVIGPKGQMINSIQEETGADIIIEDDGTIY
                     VGAADGPSAAAAVDRINAIANPQMPKVGERFLGTVVKTAAFGAFVSLLPGKDGLVHIS
                     KLGRGKRIGKVEDVVNVGDKLQVEIADVDQRGKISLVLVDDEASTPQA"
     gene            complement(2696..2959)
                     /gene="rpsO"
                     /locus_tag="VH419_11155"
     CDS             complement(2696..2959)
                     /gene="rpsO"
                     /locus_tag="VH419_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002548327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEY7044216.1"
                     /translation="MDAATKKKIIDEYATGDGDTGSPEVQVALLSRRISDLTEHLKEH
                     KHDHHSRRGLLLLVGQRRRLLNYLQHTEIQRYRSIIERLGLRR"
     gene            3238..4074
                     /locus_tag="VH419_11160"
     CDS             3238..4074
                     /locus_tag="VH419_11160"
                     /inference="COORDINATES: protein motif:HMM:NF016162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HEY7044217.1"
                     /translation="MALSLQRHLVRARADLRFHPMQTRLRAELEGHQVADTTAGFIVW
                     EPRRVVPSYAVPADDVQADLEPARAPEDEPTSAALGSDLVLTPAAPFRYHSTPGTPYD
                     VRAEDGPVAEQAAFRPDDSDLAHTVFLDFAAFDWWEENEKAISHPRDPFHRIDIRPAA
                     RHVEVRFDGRTLADSDHALILTETSLPPRWYFPRADVAMDLLEPSDHKTTCAYKGHAD
                     HFSMPASAEGDAIAWTYEKPLHEAAGVAHAVAFYNERVDVLVDGELQDRPRTPWARAR
                     GH"
     gene            complement(4083..4469)
                     /locus_tag="VH419_11165"
     CDS             complement(4083..4469)
                     /locus_tag="VH419_11165"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY7044218.1"
                     /translation="MKFLEATPVLATRDLAAALDRLGRLGFTVSSYVDPDGQGDYYGY
                     AERDGVHLHVARVDDLDPATTPISVYLYVDDADEVHAAWSEAGVEGRLHPVEDTDYGL
                     REAAYVDPDGNLVRFGSWLPGHGPDG"
     gene            4845..7619
                     /locus_tag="VH419_11170"
     CDS             4845..7619
                     /locus_tag="VH419_11170"
                     /inference="COORDINATES: protein motif:HMM:NF014214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M36 family metallopeptidase"
                     /protein_id="HEY7044219.1"
                     /translation="MNQGKAQAKHSAEKLGNYDSRAPKANRKSVEARKARMIAHASPG
                     VRALQHHLGMQGIVDIDPVTGTPRQVMRLDGYLTGRSAKPAAVIARSYVRQHPGVFKL
                     NRHEVANLRLRKDYVDIGGTHHLSFVQSVRGVPVFGNGLRAHVDKHGRLIKVDGSPVA
                     KLPTHLAAPTMSALAAQRTAVRAVFGKPSGSVKRTQAGAARTTTFSNGDQAKLVAFKT
                     RSGMRSAWQVIDMGNGWLSVIDARDGSTLFRQSMVASDNGRAWKNFPDAPRGGTQHKV
                     NLTAPGWLPNNSPRLAGNVAHVYKDINDNNAADAGEEVPPSGRRSFAYPFTPIDSDAC
                     PAGWTCSWDPNTPNSWQVNANQNAVQMFYFLGKFHDHLRKAPIGFTRTAGNFEAVDGD
                     AVDGNAIDGANINGGLPDGQHIDNANMSTPPDGNSPRMQMYLFHEPGTAFPDEDPFLA
                     GNSGDEADVVYHEYTHGLSNRLVVDANGNSTLGNVQAGSMGEAWSDWYAMDFLVNDGL
                     FRDTNAAGDLRIGEYVGWGNDLIRTQPLDCPVGTTSAKCPGTPGAGPGGYTYGDFGKI
                     IGAPEVHADGEIWAETLWDVRKALGSRLTESLVTRAMELSPANPSYLDERNAILQADL
                     VNYGGRHQDKIWKLFAARGMGWFAASVDGDDTHPAEDFSTPPPANTPRGTLTGVVTDE
                     ATGAPAAGVVVGFGGHASGFGDDYAATTDANGEYTISGIIPGTYAKVFAKGGGFDQQV
                     QTVSISSGSQSLDWALRRDWAALAGGATVVATNDDTGAPFGCGAAAMFDQSQGNGWSA
                     EAVLTGPTSIEPRFVTVQLPVAIDISDLQINPSNTCGDAGSASTGDFSVETSQDGTTF
                     TLAASGHFGIADRNMHSVPLSAGTTGVQYIRYTMIGTQVPDLGGTCPGNFSGCSFVDS
                     VELAAYGSPS"
     gene            8001..10736
                     /locus_tag="VH419_11175"
     CDS             8001..10736
                     /locus_tag="VH419_11175"
                     /inference="COORDINATES: protein motif:HMM:NF014214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M36 family metallopeptidase"
                     /protein_id="HEY7044220.1"
                     /translation="MSNYDSRSPKGSQQSLAARKARMMAHASPGVRALEKHLGMQGIV
                     DIDPVTGTPRQVMRLDGYLTGPSAKPAAAVARSYVRAHPGVFKLNRHEVAKLKLRKDY
                     VDIGGTHHLSFVQSVRGVPVFGNGLKAAVDKHGRLIRVDGSPLAHVPAQLAAPTISAL
                     AAQRTAVKAVFGKTTATVKRTQLGAARTTTFSNGDRAKLAAFKTKSGVRSAWQVIDMR
                     NGWLSVIDARSGTTLFRQNLVASDNGRAWKNFPDAPRGGTQRNVNLTSPGWLPNNSPR
                     LAGNIAHVYKDINDNDAADAGEEVPPSGRRSFAYPFTPIDSDACPDGWTCSWDPNTPN
                     SWQVNANQNAVQMFYFLGKWHDHLRNAPIGFTRSAGNFEAVDGDAVQGNAIDGANITG
                     AGLPDGQHIDNANMSTPPDGMAPLMQMYLFHQPGTAFPDEDPFLAGNSGDESDVVYHE
                     YTHGLSNRLVVDANGVSTLGNVQAGSMGEAWSDWYAMDFLVDDGLFRDTNAAGDLRIG
                     EYVGWGNDLIRTQPLDCPVGTTSPKCPGTPGAGPGGYTYGDFGKIIGAPEVHADGEIW
                     GETLWDVRKALGSRLTESLVTRAMELSPSNPSYLDERNSILQADLVNYNGRHQDKIWK
                     IFAARGMGWFAAATNGDDARPVEDFSTPPPANTPRGTLTGVVTNQDTGEPIAGAVVGF
                     GGHASGFGGDYAATTDANGQYAITGIIPGTYAKVFAQGPGFDPVVQTVSISSGTQSLD
                     WTLRRDWAALGGGATVVDFNGADFSPFGCAPGNLFDQSQASGWVSDAELTGATSIEPR
                     WIIVKMPTAVDIADLQINPSNNCGLAGSSSTGDFSVETSPDGTTWTLAASGHFGIADR
                     HMHSVPLTAGTAGVQYIRYTMLGTQVADLGGTCPGNFDGCDFVGSVELAAYGSPS"
BASE COUNT         1805 a         3688 c         3640 g         1692 t
ORIGIN      
        1 tgctgcgtgt cgtcatcgcg acacgcagca cggacaactc gggaagcgga gggctacgcc
       61 tgcggcgtgc tcgcctcgtc gtcgacgagc acgaggctga tcttgccgcg ctggtcgacg
      121 tcggcgatct ccacctgcag cttgtcgccg acgttgacga cgtcctcgac cttgccgatc
      181 cgcttgccgc gccccagctt gctgatgtgc accaggccgt ccttgcccgg cagcagcgag
      241 acgaacgcgc cgaacgcggc agtcttgacc accgtgccga ggaagcgctc ccccaccttg
      301 ggcatctgcg ggttggcgat cgcgttgatc cggtcgacgg cggcggccgc ggacggcccg
      361 tcggcggcgc cgacgtagat cgtgccgtcg tcctcgatga tgatgtccgc gccggtctct
      421 tcctggatcg agttgatcat ctgacccttg ggcccgatca ccgcgccgat cttgtcgacc
      481 gggatcttga tggtggtgac gcgcggcgcg tggtcggaca tctccgccgg gccggcgatc
      541 gcctcctcca tcacgtcgag gatcgtcagg cgcgcctcgt gggcctgctg cagcgcggcg
      601 gcgagcacgt cggacgggat gccgtcgagc ttcgtgtcga gctgcagcgc ggtgacgaac
      661 tcgcgcgtgc cggcgacctt gaagtccatg tcaccgaacg cgtcctccgc accgaggatg
      721 tcggtgagcg cgacgtactg ggtcttgccc tcgacctcgt cggagatcag gcccatcgcg
      781 ataccggcga cggccgcctt cagcggcacg ccggccgaca gcagcgccat cgtcgaggcg
      841 cagaccgagc ccatgctggt cgagccgttg gagccgagcg cctcggacac ctgacggatc
      901 gcgtagggga actcctcgcg cgccggcagc accgggatgc gggcccgctc ggcgagcgcg
      961 ccgtgcccga tctcgcgccg cttcggcgcg ccgacccggc cggtctcacc ggtcgagtag
     1021 ggcgggaagt tgtagttgtg catgtagcgg cgcgtcgtct ccggcgagag cgtgtcgagc
     1081 ttctgctcca gcgacagcat gttcagtgtc gtgatgccga ggatctgcgt ctcgccgcgc
     1141 tcgaagagcg ccgagccgtg aacccgcggg accacgccga cctcggcgga cagcgtccgg
     1201 atgtcggtga gcccccggcc gtcgatacgg acccggtcgc gcagcacgcg ttcgcgcacg
     1261 agcttcttgg tcagcgatcg gaatgccgca ccgagctcct tctcgcgacc ctcgaacagc
     1321 tcgccctgct tctcgagcac ggatgctttg atctcgtcga gcttcgactc gcggtcgagc
     1381 ttgtcggcga tcgtcagggc ttgcgcgagc tcgccggaag cggcgccctc gacggcctcg
     1441 aacacgtcgt cctggtagtc gaggaagcgg gggaactccg tcaccggctt ggcggccacg
     1501 tcggcgagct gctgctgcgc ctcgacgagc tgcttgatga cagccttcga cgactcgagg
     1561 ccctgtgcga cgacctcttc ggtcggagcc tgggcaccgc cctggacctt ctcccaggtt
     1621 gcctcggtcg actcggcctc gaccatcatg atcgcgacgt cgcctgtctc ggtgacacgt
     1681 cccgcgacga ccatgtcgaa cacggccttc tcgagctggg tgtgcgtcgg gaacgccacc
     1741 cagtcaccgt cgatcagggc cacccgggtg gcgccgatcg ggccgctgaa cggcaggccg
     1801 gacagctggg tcgacatcga ggccgcgttg atggccaaga cgtcgtacgc cgtgtcgggg
     1861 tgcagcgcca tcaccgtgat gacgacctgc acctcgttgc ggaggccgga cacgaacgac
     1921 ggccgcagcg gccggtcgat cagtcggcag gtgaggatgg cgtcctcgga cggacggccc
     1981 tcgcggcgga agaacgagcc ggggatacgc ccggcggcgt acatgcgctc ctcgacgtcg
     2041 accgtcagtg ggaagaagtc gaagtgttcc ttgggggtct tgccggcggt tgtcgccgac
     2101 agcagcatgg tctcttcgtc gatgtaggcg acggcggcgc cggcggcctg tcgggcgagc
     2161 tggcccgtct cgaaacggat ggtgcgcttg ccgtgcgcgc cgttgtcgat cactgtctcg
     2221 acggacttgg ggtcggatcc cgtcatggga ttcctttctc gaacgtgtga cgtcacacga
     2281 tgaacgccgc tggccagggg ccggacgtgg cggggcgcgc attgtgacag gcagcagcct
     2341 gcgcgggccg accttccacg tgggcgtcgg tcacgagctg tcggtcggcg gccagcgtgc
     2401 ggccgggcat gtgaccgagt ggtcgctacg tcgccgaggc cggtcttcga tcgaggcttg
     2461 cgggcggcat ccgtcgtacg acggcggcgt cccgagagcc actaccgagg accggcatcc
     2521 tcgctgttgc gaacgtcagc gcgtgacgtc actgggtggt ggtgcctgcg tacggcagtc
     2581 gtacgtcgac acccgtcagt gttgcgtgaa ctctcttcag ttttcgtcct gcacgggaat
     2641 gcccggcagt gatgcgaaga agcggccgtc cccgacgggg aggccgcttc caagatcaac
     2701 gacgcaggcc gagccgctcg atgatcgagc ggtagcgctg gatctccgtg tgctgcaggt
     2761 agttgagcag acgacggcgc tgacccacga ggagcagcag gccacgacgg ctgtggtggt
     2821 cgtgcttgtg ctccttcaag tgctccgtga ggtccgagat ccggcggctc agcagcgcca
     2881 cctgtacctc gggcgagccg gtgtcaccgt cgccggtggc gtactcgtcg atgatcttct
     2941 tcttggtcgc agcgtccagt ggcacgcgat ctcccttcgg gggcttgttg cgcggcgcgc
     3001 cggggcctgt tcacccgggc acatcggttt ccgcggccgt tccacggcac cctcaagggt
     3061 atcggcgcac ccgccatgcc tccaaatccg ccaccgagac tccccgcgga ttgcggggag
     3121 ttccgggatt gcgtcgtcca gaatcgcgga acctcccgca attcgcggtc ggactgggct
     3181 gggtcggcgg ctggatgagg cgttgacgag gccggggagg ccgcgcatgc tcgacggatg
     3241 gccctctcgt tgcagcgtca cctcgttcgc gcccgcgccg acctgcgctt ccacccgatg
     3301 cagacccggc tgcgtgccga gctcgagggc caccaggtcg ccgacacgac cgccggcttc
     3361 atcgtctggg agccgcgtcg ggtcgtgccg tcctacgccg taccggccga cgacgtccag
     3421 gccgacctgg agccggccag ggcgccggag gacgagccga cctcggctgc gctggggagc
     3481 gatctcgtgc tgaccccggc ggcgccgttc cgctaccact cgacgcctgg tacgccgtac
     3541 gacgtccgcg ccgaggacgg cccggtcgcc gagcaggctg ccttccggcc cgacgactcc
     3601 gacctggcgc acaccgtctt cctcgacttc gccgcgttcg actggtggga ggagaacgag
     3661 aaggcgatca gccatcctcg cgacccgttc caccgcatcg acatccgtcc ggccgcacgc
     3721 cacgtcgagg tgcgcttcga cggacggacg ttggccgact ccgaccacgc gctcatcctc
     3781 accgagacct cgttgccacc gcgctggtac ttcccgaggg cggacgtggc gatggacctg
     3841 ctcgagccgt ccgaccacaa gacgacgtgt gcctacaagg gacacgccga ccacttctcg
     3901 atgcctgcca gcgccgaggg tgacgcgatc gcgtggacct atgagaaacc actgcacgag
     3961 gctgccggcg tcgcccatgc tgtggcgttc tacaacgagc gcgtcgatgt gctggtcgac
     4021 ggcgagttgc aggaccggcc gcgaacgccg tgggctcgcg cccgcggtca ctgacctgtc
     4081 ggtcagccgt ccggtccgtg ccccggtagc cacgacccga acctgaccag gttgccgtcc
     4141 ggatcgacgt acgccgcctc acgcagtccg tagtcggtgt cctcgaccgg atgcaaccgg
     4201 ccttccacgc cggcctcgga ccaggcggcg tgcacctcgt cggcgtcgtc gacgtagagg
     4261 tagacggaga tcggggtcgt cgcgggatcg aggtcgtcca cacgggcgac gtggagatgg
     4321 accccgtcac gctccgcgta cccgtagtag tcgccttgac cgtcgggatc gacgtacgac
     4381 gacaccgtga accccagccg gcccagtcgg tccagcgcgg ctgcgaggtc acgcgtcgcg
     4441 agcaccgggg tcgcttccag aaacttcacg ctcacgactc tgccagatca cggccagttg
     4501 gccaccggtc cgatctcgcc gagcggcgga cgtgacggcc gaccagaaaa gtccgcccgg
     4561 ggtatggctt gacgataaca atcaggataa gaatgccggt ggacaactgg catcacgtcg
     4621 ttctgcgggg cactgcggcg ggtgcacaga tgggtgggac ccaccgattc ttcgccgctc
     4681 gccccccaca agggaaaggg gaagtcgtga agcggtccag attgttgtcg tccgttcccc
     4741 gcaagcgggt tgggcaggtg gtgggccttc tcagcgtcgc tgcgctcgcc ctcgcaacac
     4801 tgccgggaac ggcaaccgca cagagcccga cacctggggc tgcggtgaac caaggtaagg
     4861 cccaggccaa gcactcagct gaaaagctgg gcaactacga ctcccgagcc cccaaggcca
     4921 accggaagtc tgtggaagcg cgcaaggcca ggatgatcgc tcacgcgagc cccggcgtac
     4981 gcgcgctcca gcatcacctc ggcatgcagg ggatcgtcga catcgacccg gtcaccggta
     5041 cgccgcgaca ggtcatgcga ctggacgggt acctcaccgg acgtagcgcg aagccagcgg
     5101 ccgtgatcgc gcgctcctac gtcaggcagc accctggcgt gttcaagctg aatcggcacg
     5161 aggtcgccaa cctgcggctc cgcaaggact acgtcgacat cggtggcacg caccacctca
     5221 gcttcgtcca gagcgtccgt ggcgttccgg tcttcggtaa cgggctgcgg gcacatgtcg
     5281 acaagcacgg tcgactgatc aaggtggacg ggtcccccgt cgccaagctc ccgacacacc
     5341 tggccgcacc gacgatgagt gcactggccg cgcagcggac cgccgtcagg gcggtgttcg
     5401 gaaagccgag cggcagcgtg aagcgcacgc aagccggtgc tgcccgcacg accacgttct
     5461 cgaacggcga ccaggccaag cttgtcgcgt tcaagacgag gtccggcatg cggtccgcct
     5521 ggcaggtgat cgacatgggc aacggctggc tgagcgtgat cgacgcgcgt gacgggtcga
     5581 cgctgttccg gcagagcatg gtcgcctcgg acaacggccg ggcctggaag aacttcccgg
     5641 acgcgccgcg tggtggcacc cagcacaagg tcaacctgac cgcgcctggt tggctgccga
     5701 acaactcgcc gcgactggcc ggcaacgtcg cccacgtcta caaggacatc aacgacaaca
     5761 acgccgccga cgcaggcgag gaagttccgc cgagtgggcg caggagcttc gcctacccgt
     5821 tcacgccgat cgacagcgac gcctgcccgg ccggctggac ctgttcctgg gacccgaaca
     5881 ctccgaactc ctggcaggtg aacgccaacc agaacgccgt gcagatgttc tacttcctcg
     5941 gcaagttcca cgaccacctg cgcaaggcgc cgatcggctt cacccggacg gcgggcaact
     6001 tcgaagcggt tgacggcgac gccgttgacg gcaacgcgat cgacggtgcc aacatcaacg
     6061 gcggcctgcc ggacggtcag cacatcgaca acgccaacat gagcacaccg cctgatggca
     6121 actcgccgcg gatgcagatg tacctgttcc acgagcccgg gaccgcgttc ccggacgaag
     6181 acccgttcct tgccggcaac tccggtgacg aggccgacgt ggtctaccac gagtacacgc
     6241 acggtctgtc gaaccgtctg gtcgtcgacg ccaacggcaa ctcgacgctg ggcaacgttc
     6301 aggcgggctc gatgggcgag gcgtggagcg actggtacgc catggacttc ctcgtgaatg
     6361 acggcctgtt ccgcgacacc aatgccgcgg gtgacctgcg gatcggtgag tacgtcggct
     6421 ggggcaacga cctgatccgc acccagccgc tggactgccc ggtcgggacc acttcggcga
     6481 agtgcccggg gactccggga gccggtccgg gtgggtacac ctatggcgac ttcggcaaga
     6541 tcatcggtgc acccgaggtg catgccgacg gcgagatctg ggccgagacc ctgtgggatg
     6601 tgcgcaaggc actcggtagc aggctgaccg agtcgctcgt gacccgggcg atggagctgt
     6661 caccggccaa cccgtcgtac ctcgacgagc gcaacgccat cctgcaggcc gacctggtca
     6721 actacggtgg ccgccaccag gacaagatct ggaagctctt cgcggcccgc ggtatgggct
     6781 ggttcgccgc ctccgtcgac ggagacgaca cccacccggc cgaggacttc tcgacgccgc
     6841 cgccggcgaa cacaccgcgt ggcacgctca ccggtgtcgt caccgacgag gccacgggag
     6901 ctccggccgc gggagtggtc gtcggcttcg gcggacacgc ctccgggttc ggtgacgact
     6961 acgccgcgac gaccgacgcc aacggtgagt acacgatcag cgggatcatc cccggcacct
     7021 acgcgaaggt cttcgcgaaa ggtggtggct tcgaccagca ggtccagacc gtgtcgatct
     7081 cgtccgggag ccagtcgctc gactgggcgc ttcgtcgtga ctgggcagcc ctcgccggcg
     7141 gtgcgacggt cgtcgccacc aacgacgaca cgggtgctcc gttcggctgt ggcgctgcgg
     7201 cgatgttcga ccagtcgcag gggaacggtt ggagcgccga agcggtcctg acaggcccga
     7261 cctcgatcga gccacgtttc gtcacggtgc agctgccggt cgcgatcgac atcagcgacc
     7321 tgcagatcaa cccgtccaac acctgtggtg acgcgggaag tgcgtcgacc ggtgacttct
     7381 cggtggagac gtcgcaggac ggcaccacgt tcaccctcgc ggcctcgggg cacttcggca
     7441 tcgctgaccg caacatgcac agcgtgccgc tgagcgccgg gaccaccggt gtccagtaca
     7501 tccgctacac catgatcggc acccaggtgc ccgacctcgg cgggacgtgc ccgggcaact
     7561 tcagcggttg ttcgttcgtc gacagcgtcg agctggcggc gtacggatct cccagctgat
     7621 cagttcctga cagcctcatc ggctgtccga aaggagaatc ctggacgtac gacaacacgt
     7681 ccacgacggt gcccggtgcg cgttcgcgcc cgggcaccgc gccgttcgag ggacccactc
     7741 accgccccac atggctgctg ttgcagcacg gcagcacaag cggttgactc ggctcggggt
     7801 cgtctgaccc cgacgtgaaa ggggaagatg tgaagcggtc cagattgttg tcgtccagcc
     7861 gaaagcgcgc tggacaggtg gtcggccttc tcggcgtcgc agcgctcgcc ctggcgatgc
     7921 tgccggggat ggccaccgca cagagcccgt caccccgggc tgcggtgaac caaaccaagg
     7981 ccacgagctc tggtgacaaa gtgagcaact acgactcccg gtccccgaaa ggcagccagc
     8041 agtcgctggc ggcgcgcaag gcccggatga tggcgcatgc cagtcccggc gtacgcgccc
     8101 tcgagaagca tctcggcatg cagggcatcg tcgacatcga cccggtcacg ggtacgccgc
     8161 gacaggtgat gcgcctcgac ggctacctca ccggtccgag cgcgaagccg gccgcagccg
     8221 tcgcccgctc gtacgtccgg gcacaccccg gcgtgttcaa gctgaaccgc cacgaggtgg
     8281 cgaagctgaa gctgcgcaag gactacgtgg acatcggtgg gacgcaccac ctcagcttcg
     8341 tccagagcgt ccgcggcgtg ccggtgttcg gcaacggcct caaggcggct gttgacaagc
     8401 acggtcggct gatcagggtg gacggctcgc ccttggcgca cgttcccgcg cagttggccg
     8461 cgccgacgat cagtgcgctg gctgcacagc ggaccgctgt caaggcagtg ttcggcaaga
     8521 cgaccgcaac ggtgaagcgc acgcagctcg gtgccgcacg aacgaccacg ttctcgaacg
     8581 gtgaccgggc caagcttgcc gcgttcaaga cgaaatccgg cgtccggtcc gcctggcagg
     8641 tgatcgacat gcgcaacggc tggctgagcg tgatcgacgc ccggagcggg acgacgttgt
     8701 tccggcagaa cctcgtcgcg tcggacaacg ggcgggcctg gaagaacttc ccggacgcac
     8761 cccgtggtgg cacccagcgc aacgtcaacc tgacctcgcc tggttggctg ccgaacaact
     8821 cgccacgact ggccggcaac atcgcccacg tctacaagga catcaacgac aacgatgccg
     8881 ctgacgcagg cgaggaggtc ccgccgagcg ggcggagaag ctttgcgtac ccgttcacgc
     8941 cgatcgacag cgacgcctgc ccggacggct ggacctgctc ctgggacccg aacacgccca
     9001 actcctggca ggtgaacgcg aaccagaacg ccgtgcagat gttctacttc ctcgggaagt
     9061 ggcacgacca cctgcggaac gccccgatcg gcttcacccg cagcgcaggg aacttcgagg
     9121 cggtcgacgg tgacgccgta caaggcaacg cgatcgacgg agcgaacatc accggcgccg
     9181 gactgccgga cggtcagcac atcgacaacg ccaacatgtc cacgccaccg gacggcatgg
     9241 caccgctgat gcagatgtac ctgttccacc agccgggaac cgccttcccg gacgaggacc
     9301 cgttccttgc cggcaactcc ggcgacgagt ccgacgtcgt gtaccacgag tacacgcatg
     9361 gtctgtcgaa ccggctggtc gtcgatgcca acggcgtctc gaccttgggc aacgtccagg
     9421 ctgggtcgat gggcgaggcg tggagcgact ggtacgccat ggacttcctc gtcgatgacg
     9481 ggctgttccg cgacaccaac gccgcgggtg acctgcggat cggtgagtac gtcggttggg
     9541 gcaacgacct gatccgcacc cagccgctcg actgcccggt cgggacgacc tccccgaagt
     9601 gtccgggcac gcccggtgcc ggaccaggtg ggtacaccta cggcgacttc ggcaagatca
     9661 tcggtgcacc cgaggtgcac gccgacggtg agatctgggg cgagacgctc tgggatgtgc
     9721 ggaaggccct gggtagcagg ctgaccgagt cgctggtgac gcgggcgatg gaactgtcgc
     9781 cgtcgaaccc gtcgtacctc gacgagcgca actcgatcct gcaggccgac ctggtcaact
     9841 acaacggccg tcaccaagac aagatctgga agatcttcgc ggcccgcggc atgggctggt
     9901 tcgccgccgc cacgaacgga gacgacgcgc gcccggtgga ggacttctcg acgccgccgc
     9961 cggcgaacac gccgcgtggc acgctgaccg gagtcgtcac caaccaggac accggtgagc
    10021 caatcgccgg tgcggtcgtg ggcttcggtg gacacgcctc cggcttcggc ggcgactacg
    10081 ccgcgacgac cgacgccaac ggtcagtacg cgatcaccgg gatcatcccg ggcacgtatg
    10141 cgaaggtctt cgcacaaggt ccagggttcg accctgtggt ccagaccgtc tcgatctcgt
    10201 ccggcactca gtcgctcgac tggacgctgc ggcgtgactg ggcggccttg ggcggcggtg
    10261 caacggtcgt ggacttcaac ggcgcggact tcagtccgtt cggctgtgcg cccgggaact
    10321 tgttcgacca gtcgcaggca agcggctggg tctcggacgc ggagctgacc ggggccacgt
    10381 cgatcgaacc gcggtggatc atcgtcaaga tgccgaccgc ggtcgacatc gcagacctgc
    10441 agatcaaccc gtccaacaac tgtggtctgg ctggaagctc gtccaccggc gacttctcgg
    10501 tcgagacctc gccggacggc acgacgtgga cgttggccgc atccggacac ttcggcatcg
    10561 ctgaccgcca catgcacagc gtgccgttga ccgcggggac ggccggtgtc cagtacatcc
    10621 gctacacgat gctcggcacc caggtcgccg acctcggcgg cacctgcccc ggcaacttcg
    10681 acggctgcga cttcgtcggc agcgtcgagc tggcggcgta cggatcaccg agctgagtgt
    10741 tcctggcagc caaccggctg tctgagagga ggacgctgaa cggcaggaag tgcgttcacg
    10801 ctggtgcccg gatgcgcacg catcc
//
LOCUS       DATBFZ010000371         8685 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_912169, whole genome shotgun sequence.
ACCESSION   DATBFZ010000371 DATBFZ010000000
VERSION     DATBFZ010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 8685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8685
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_912169"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..82)
                     /locus_tag="VH419_11180"
     tRNA            complement(10..82)
                     /locus_tag="VH419_11180"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(47..49),aa:Arg,seq:cct)
     gene            complement(141..1040)
                     /locus_tag="VH419_11185"
     CDS             complement(141..1040)
                     /locus_tag="VH419_11185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044221.1"
                     /translation="MSTEVDTSVVEWLLEGDPSIRWRVMHDITSSSARAVRRERARVA
                     TEGWGARLLAAQDDDGRWDGGVYSPKWTSTTYTLLRLLWLGLPPRHPGALRGCERLWE
                     WRGRWRVPETCIVGILVRLTSYHEYAADGLDDLTAYLMEQQLADGGWNCATLTDKQKH
                     GSFHTSIQALEALVGYQDAGGALDVSGSVARGREFFLTHRLYRSHRTGEVAIPRSTRF
                     PAFPEWHFDVLRGLEHFCACATERDERLADAVDVVRRARRADGRWPTYASYAGRQWFE
                     FEPPGPSRWNTYRALRVLQWWDG"
     gene            1122..2051
                     /gene="mmuM"
                     /locus_tag="VH419_11190"
     CDS             1122..2051
                     /gene="mmuM"
                     /locus_tag="VH419_11190"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020386429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047150 - betaine-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase"
                     /protein_id="HEY7044222.1"
                     /translation="MTTTGPHLEAVGPIRLDAERVIVLDGGMSNALEDHGHDLSGTLW
                     TARLLRDAPDGIAAVHRVYFEAGARVATTASYQASVPGFVADGMAPKEAERLIRRSVT
                     IARDVRDRMSGDGTRRWVAASVGPYGAVLADGSEYRGRYGLTRVALRDFHLPRIELLA
                     SADPDFLAVETIPDLDEAEVLAELLQELDLPAWFSFSAAGLETRAGQPLEDAFAVAAS
                     AGGVFAVGVNCCTPADVLSAVDLAAKVTGKPVVAYPNSGQTWDARSRRWNGEAAYDVQ
                     LARDWISAGAAYVGGCCRVGPATIAALADAVEG"
     gene            complement(2048..2275)
                     /locus_tag="VH419_11195"
     CDS             complement(2048..2275)
                     /locus_tag="VH419_11195"
                     /inference="COORDINATES: protein motif:HMM:NF024788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLD nuclease N-terminal domain-containing
                     protein"
                     /protein_id="HEY7044223.1"
                     /translation="MPRANKKNWSDLTPHQQKAIILGGAVELVLTTLALRDLARRPSD
                     QVRGPKALWVLSFAIQPFGPLAYFVAGRKVA"
     gene            2370..3359
                     /locus_tag="VH419_11200"
     CDS             2370..3359
                     /locus_tag="VH419_11200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044224.1"
                     /translation="MEEGSGSERPALLPVRTASADDQALTVLAHQLTQAIFGAAALAS
                     EVVLRSLAESMPARIGSGPATFVRAHDAVDVLLGLGWRAAYVTDRVVGVGVAAAAPVV
                     RFTLSPPLVPRRLTLGDMLRRAGTSWDGERSRAVQSLSDLSATFTPAASDVAVRLVDV
                     DKLVSKVLAEVNVQRLAEGLLAQLDMNELIADLVTQIDVRRLAEQMLTDVDLTPVVEQ
                     VIEQVELDTIVDSALDELDLTDVVVERVDLQRVIDSALDRMDLTELILQRVDLVRVSE
                     FVVNGIDLPELIRDSTGSMAAETVNEIRLQGIDADRAVARIVGRILRRTPEQP"
     gene            3356..3880
                     /locus_tag="VH419_11205"
     CDS             3356..3880
                     /locus_tag="VH419_11205"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEY7044225.1"
                     /translation="MTGDTAGAGPRPVRPADVRHEGDAYQGMPAGVVTRSLAAAVDGL
                     VAGAIELGLWGGYAALVFLLDPHHFTFPSPPVLSFVGLFGVVAFVYLSAAWAMSGRTL
                     GGQLLGLRVQSSDGGRPAWLTSMARAALYIVFPIGLFWSAVSARNRSVQDMLLRTSVV
                     YDWGHRPPQSPPTG"
     gene            complement(3989..5116)
                     /locus_tag="VH419_11210"
     CDS             complement(3989..5116)
                     /locus_tag="VH419_11210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044226.1"
                     /translation="MFGSQAIETAIGLAVMFFVIATAASMVVEVISRLLQKRARDLEH
                     AVKGLLGDQPDGGEAHALRLFTNTSIYRSARQAAGRAWFGTRKPSYLSAKAFADATVE
                     MLTEPDGKTLRKLDRLPMNLRDRLKPLVRESGEDLVAIKSGLERWFDEAMQRTEGSYK
                     RWSTLVLFIVGLVFAVGGNASTVGVAQHLWEGSATREAVVDAADQVVSDQGSPEDQLE
                     SVASTTSSLQELQLPVGWDATSRAHFSLDPADLTPTGLGMIAGWLLTALLVMLGAPFW
                     YDLLSKLVALRTSGSKPPTAGADPASSTSLRLAAQPAETTTAVAAVQAPAPEVPHPRA
                     GEPAQEEQEQEQAPERPPPRPVHDDAAIEEILANLLRLELP"
     gene            complement(5126..6493)
                     /locus_tag="VH419_11215"
     CDS             complement(5126..6493)
                     /locus_tag="VH419_11215"
                     /inference="COORDINATES: protein motif:HMM:NF021508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2235 domain-containing protein"
                     /protein_id="HEY7044227.1"
                     /translation="MTEEHSPQRAAKRLVICCDGTWNAPDNANITNIEKIARSIETNA
                     ANSGGVQQLVEYVQGVGVGYKTDRLLGGAFGFGLFNNVRTAYRFLALNYEVGDEIFIF
                     GFSRGAYTARSLAGMVGYVGLLTGDALVRGQLRRALDLYKNRDTAQDTASKAELKRFH
                     EENCHDVTPITFIGVFDTVGALGVPGSVLFPEHQFHDIDLGNHVLCARHAVAIDERRM
                     TFAPCLWTVPADQNGTVTFRSGTPTEQTVDRVKQVWFEGVHSDVGGGYPENGLSCTTL
                     LWMADEAKAAGLVFDEHRLGFYVDRAKKPVRNNSLTFWFRIVNIRERMRPRRGVDRRA
                     FVNGLRSLEPPVVPLKDHPERTMVLPGRVSSTAWTAFDDAAEAYRPANLAAFITGHTP
                     PKIGETSESVILLPREVVAAPSSSDGSPDAVDDSAGPPEGSSAEVAVPQPRVGDPESD
                     VAYRR"
     gene            complement(6577..7992)
                     /locus_tag="VH419_11220"
     CDS             complement(6577..7992)
                     /locus_tag="VH419_11220"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEY7044228.1"
                     /translation="MSRCVRVACCLLLVAAMSSLAAAGEASDRAASHVGIVVPAGGDW
                     KQFAGRAVHHNHAVAEGAFTQQTVGRLHEAWRTTFGTSASSTGGAAVAAGQAFIGGAD
                     GVLSAFSLQACATGSCTPLWRGVTKNGIHGTPAIVTDGLVLVGSADHFLYAFHTDGCG
                     RRTCPPVWRGKLQDSALASVAVFRGRAYIGDAGGHVYAFHAHGCGHPVCQPLWTGIAG
                     PNLQFNTIPAVADGHVFIGAFFNTPDDASGRLFSFATLGCGKATCRPEWRADMRGQVD
                     GTLSPLVLGDTVFMGSGTRFSNVPNGPVHLFAFDVAGCGKRFCHPVRSYRTGDADLTG
                     GLAVANGVIYAGSQSTPDPNTIGVFMAFPADGCGAAVCDPLWTAVNFASGFESPPVVS
                     GDVVFVAKGPASGFPVDQAVLSYPADGCGQPTCTWLSFTSLGDQQFYLGSPIAVARHT
                     VFVPTEASADGTDDLVALTVE"
     gene            complement(8130..>8685)
                     /locus_tag="VH419_11225"
     CDS             complement(8130..>8685)
                     /locus_tag="VH419_11225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044229.1"
                     /translation="TDPAKSKELLEQAGAMNYPIQFLYSQDDPVSVDVKDAIVKGLTA
                     GGFKPEPVATTVADLSTVRSDPDAKINVRSAGWCSDWPTGSSWFPPVIKSTNLKEEGL
                     GANYAVFNEPAVDKQISDIQLMPLDKQPGAWNDLDKEVLTKYFPLFNTGYAGVAMMHG
                     SKIEGMHNDNTYGMPTWRDIWVKQ"
BASE COUNT         1334 a         2918 c         2983 g         1450 t
ORIGIN      
        1 ttttgctagt gcccccggca ggattcgaac ctgcgacaca cggtttagga aaccgttgct
       61 ctatcccctg agctacgggg gcgctgtatg actgacgagc agggtagtcg atggtcacgt
      121 gcgctcacgc ggctggcggc tcagccgtcc caccactgca gcacgcgcag cgcgcggtag
      181 gtgttccatc ggctcgggcc aggcggctcg aactcgaacc actggcggcc ggcgtacgac
      241 gcgtacgtcg gccacctgcc gtcggcgcga cgagctcggc gtacgacgtc gacggcgtcg
      301 gcgagccgtt cgtcgcgctc ggtcgcacac gcgcagaagt gctccagtcc gcggagcacg
      361 tcgaagtgcc actcgggaaa cgccgggaag cgggtgctcc gtgggatcgc cacttcgcct
      421 gtccggtgag accggtagag ccgatgtgtc aggaagaact cgcgcccgcg cgcaaccgac
      481 ccggagacgt cgagcgcgcc gccggcgtcc tggtacccca ccagcgcttc cagcgcctgg
      541 atgctggtgt ggaaggagcc gtgcttctgc ttgtcggtca aggtggcgca gttccatccg
      601 ccgtcggcga gctgctgctc catcaggtac gccgtcaggt cgtcaagtcc gtccgcggcg
      661 tactcgtggt agctcgtcag ccgcaccagg atcccgacga tgcaggtctc tggcacccgc
      721 catcgtcccc gccactccca gaggcgttca catcctcgga gcgcgcccgg atgccgtggc
      781 ggaagaccga gccacaacag ccgaagcagc gtgtaggtcg tcgacgtcca cttcggcgag
      841 tacacgccgc catcccaccg gccgtcgtcg tcctgcgcag cgaggagtcg tgcaccccac
      901 ccctccgtcg cgacccgagc tcgctctctg cgcaccgccc gcgcgctgga cgaggtgatg
      961 tcgtgcatga cccgccaccg gatcgacggg tcgccttcga gcaaccactc gacgaccgac
     1021 gtgtcgacct cggtgctcat gtcgacattg tgggcgcact cgccctcgga atcgacacgc
     1081 ggacgtcgat tgccggtcgc gtgcgcgcga gagactgagg tgtgacgaca acggggccac
     1141 acctcgaagc agtcgggccg atccgactcg acgcagagcg cgtgatcgtg ctcgacggcg
     1201 gtatgtccaa cgcgctggag gaccacggcc acgacctctc cgggacgctg tggaccgcgc
     1261 ggctgcttcg tgacgcgccc gacgggatcg ccgccgtgca tcgtgtgtac ttcgaggccg
     1321 gcgcacgggt cgcgaccact gccagctacc aggcgagcgt gcccgggttc gtggcggacg
     1381 ggatggcgcc gaaggaggcc gagcgactga tccggcgcag cgtcacgatc gcccgcgacg
     1441 tacgcgatcg catgtcgggg gacggcacgc gacgctgggt cgcagcgtcg gtggggccgt
     1501 acggcgcagt cctggcggac gggtcggagt accgcggccg ctacgggctg acccgtgtcg
     1561 cgctgcgtga cttccacctg ccgaggatcg agttgttggc gtcggccgac cccgacttcc
     1621 tggctgtcga gacgatcccc gacctcgacg aggccgaggt tctcgccgag ctcctgcaag
     1681 agctcgacct gccggcctgg ttctcgttct cggccgccgg cctcgagacc cgcgccgggc
     1741 agccgctcga ggatgcgttc gccgtggcgg catccgccgg tggtgtgttc gcggtcgggg
     1801 tgaactgctg tacgccggcc gacgtgctgt cggctgtcga cctggcggcc aaggtaaccg
     1861 gcaaacccgt cgttgcatat ccgaacagcg gtcagacgtg ggacgcccgg agccgacggt
     1921 ggaacggcga ggcggcgtac gacgtacagc tcgctcgcga ctggatcagt gctggagcgg
     1981 cgtacgtcgg cggctgttgc cgcgtcggtc ccgctaccat cgcggccctg gccgacgccg
     2041 tcgagggcta ggcgaccttc ctgcccgcga cgaagtaggc cagcggtccg aacggttgga
     2101 tcgcgaacga cagcacccac agcgctttcg gtcctctgac ctggtcgctg ggacgtcgcg
     2161 ccaggtcacg cagcgccaac gtcgtcagca ccagctcgac cgcgccgccg aggatgatcg
     2221 ccttctgctg atgcggcgtc aggtcgctcc agttcttctt gttcgcacga ggcatgcaac
     2281 cgacgctagc gcgcaccgtc gatgctcacc cccgccgggt gagtcatggg tattgacgct
     2341 gctggatgct gggagcagga ggtgcgcgcg tggaagaagg gagcgggagc gagcggccgg
     2401 cgctgctgcc cgtccgaacc gcctcggctg acgaccaggc gctgacggtg ctggcgcacc
     2461 agctgacaca ggcgatcttc ggcgcggcag cgcttgcgtc ggaggtggtt cttcgttcgc
     2521 tggccgagtc gatgcctgct cggatcggca gcggtccagc caccttcgtc cgcgcgcacg
     2581 acgccgtcga cgtgcttctc ggactcggat ggcgagcggc gtacgtgacc gaccgcgtgg
     2641 tgggtgtcgg cgtcgccgcg gctgcgccgg tggtgcgatt caccctgtcg ccgcccctgg
     2701 tgccgcgtcg gctgaccctt ggggacatgc tcaggcgggc cggcacgtcg tgggacggcg
     2761 agcggtccag ggctgtgcag tccctgtcgg acctgtcggc gacgttcacc ccggccgcga
     2821 gcgacgtcgc ggtcaggctc gtcgatgtcg acaagctcgt gtccaaagtg cttgcagagg
     2881 tgaacgtcca acggctggcc gaggggctgc tggcccagct cgacatgaac gagcttatcg
     2941 ccgatctcgt tacacagatc gacgtacgcc gactcgccga acagatgttg accgacgtgg
     3001 acctgacacc ggtcgtcgag caggtcatcg agcaggtgga gctggacacg atcgtcgaca
     3061 gtgcgctgga cgagctcgac ctgaccgacg tcgtggtcga acgggtcgac ctgcaacggg
     3121 tgatcgactc cgcgctggat cgcatggacc tgaccgagtt gatcctgcag cgggtggatc
     3181 tcgtccgggt atccgagttc gtcgtcaacg ggatcgacct tcctgagctg atccgcgact
     3241 cgaccggctc gatggcggcg gagacggtca acgagatcag gttgcagggc atcgacgcgg
     3301 accgcgccgt tgcccggatc gtcggccgga tccttcggcg tacgccggag cagccgtgac
     3361 cggcgacacc gcaggagcgg ggccgcggcc ggtacgcccg gccgacgtca ggcacgaggg
     3421 tgacgcctac cagggcatgc cggccggtgt cgtcactcgt tcgctggcgg cagcagttga
     3481 cgggttggtg gcgggtgcca tcgagctcgg actgtggggc ggctacgccg ccctggtgtt
     3541 cttgttggat ccgcatcact tcacattccc gagcccgccc gtgctgtcgt tcgtcggctt
     3601 gttcggcgtc gtggcgttcg tctacctctc cgctgcctgg gcgatgtcgg gccgcacctt
     3661 gggtggccag cttctggggc tacgtgtcca gtcaagtgac ggcgggaggc cggcctggtt
     3721 gacgtcgatg gcgcgggccg cgctgtacat cgtcttcccc atcggcctgt tctggtcggc
     3781 ggtcagcgcg cgcaaccgtt cggtccagga catgctgctg cggacgagcg tcgtctacga
     3841 ctggggccac cggcctccgc agtcgcctcc gaccggctaa ccgggcagcg ttcgccagcg
     3901 atggcgatcg gcggatcctc gtgctcgagg tggcaatcga agggcgacac gccggcagaa
     3961 agcaacgaaa cctgccacct cggcggggtc agggcagctc gagtcggagc aggtttgcca
     4021 ggatctcctc gatcgcagca tcgtcgtgga ccgggcgtgg cgggggtcgt tcgggggcct
     4081 gttcctgttc ctgctcctcc tgcgccggtt cgccggcccg cgggtggggc acctcgggcg
     4141 ccggtgcctg caccgctgca accgcagtgg tggtctccgc cggctgggcg gcgaggcgca
     4201 ggctggtcga ggaggcggga tcagctccgg cggtgggcgg cttgctccca ctggtgcgca
     4261 acgccaccaa cttgctgagc aggtcgtacc agaacggtgc gcccagcatc accagcagtg
     4321 cggtgaggag ccagccggcg atcatgccga ggccggtcgg cgtcaggtca gcagggtcga
     4381 gcgagaagtg tgcgcgggac gtcgcgtccc agccgaccgg cagctggagc tcctggagcg
     4441 acgacgtggt gctggcaacc gactcgagct gatcttcggg tgacccctgg tcggagacga
     4501 cctggtcggc cgcgtcgacg accgcctctc gcgtcgccga cccctcccag aggtgttgtg
     4561 ccacgccgac cgtcgacgcg ttgccgccga cggcgaagac cagtccgacg atgaacagca
     4621 cgagcgtgga ccagcgcttg taagacccct cggtccgctg catcgcctcg tcgaaccagc
     4681 gttcgagccc cgacttgatc gccacgagat cctctccaga ctcacgcacc agtggcttca
     4741 gccggtccct caggttcatc ggaagccggt ccagcttgcg cagcgtcttg ccgtccggct
     4801 cggtcaacat ctcgacggtg gcgtcagcga aggctttcgc cgacaggtag ctcggcttgc
     4861 gcgtgccgaa ccaggcacga ccggcagcct gccgcgcgga ccggtagatc gacgtgttcg
     4921 tgaacagccg cagggcatgg gcctcgccgc catcgggttg gtccccgagc agtcccttca
     4981 cggcgtgttc gagatcgcgt gcacgctttt gcaggagtcg cgagatcacc tccacgacca
     5041 tggatgcagc ggtcgcgatc acgaagaaca tcaccgcgag accgatcgcg gtctcgatgg
     5101 cctgcgaccc gaacatgacc gtccctcagc gccggtaggc cacgtcggac tcggggtcgc
     5161 cgactctggg ctggggtacg gcgacctctg cggacgaccc ctccggcggc ccggcggaat
     5221 cgtccacggc atccggtgaa ccgtccgagg acgatggtgc ggcgaccact tcccgcggca
     5281 gcaggatgac cgactccgaa gtctcgccga tcttcggcgg ggtgtggccg gtgatgaacg
     5341 ccgcaagatt cgccgggcgg taggcctccg ccgcgtcgtc gaaggccgtc catgccgtcg
     5401 acgacacccg cccgggtagc accatcgttc gctccgggtg gtccttgagc gggacgactg
     5461 gaggttcgag gctgcggagt ccgttcacga aggcgcgcct gtcgacgcca cgccgcggcc
     5521 tcatccgctc gcggatgttg acgatgcgga accagaacgt caacgagttg ttccgcaccg
     5581 gcttcttggc ccggtcgaca tagaagccga gccggtgttc gtcgaagacg agtccggcgg
     5641 ctttcgcctc gtcggccatc cacagcaatg tcgtgcacga cagcccgttc tccgggtagc
     5701 cgccaccgac gtcggagtgg acgccctcga accacacctg tttgacgcga tcgaccgtct
     5761 gctcggtcgg cgtacccgag cggaacgtca ccgtcccgtt ctggtcggcg ggtacggtcc
     5821 acaagcacgg cgcgaacgtc attcggcgct cgtcgatcgc gaccgcatgc cgtgcgcaca
     5881 gcacgtggtt gccgaggtcg atgtcgtgga actggtgctc ggggaagagc acgctgccag
     5941 gtacgccgag agcccccacg gtgtcgaaga caccgatgaa ggtgatcggc gtgacgtcgt
     6001 ggcagttctc ctcgtggaac ctcttcagct ccgccttcga ggccgtgtcc tgcgccgtgt
     6061 cccggttctt gtagaggtcc aatgctcggc gcagctgccc gcgtacgagt gcatccccag
     6121 tgagcaaacc gacgtacccg accattccgg caagactgcg tgcggtgtat gcgccccggc
     6181 tgaacccgaa gatgaagatc tcgtctccca cctcgtagtt cagggccagg aaccggtatg
     6241 ccgtccggac gttgttgaac agtccgaacc cgaacgcccc gccaagcagt ctgtccgtct
     6301 tgtacccgac gccgacgccc tggacgtact cgacgagctg ttgcacgccg cccgaattgg
     6361 ccgcgttggt ctcgatggac cgggcgatct tctcgatgtt cgtgatgttg gcgttgtccg
     6421 gcgcgttcca tgtgccgtcg cagcagatca ccaaccgctt cgcggctcgt tgcggcgagt
     6481 gctcctctgt catcgtgggc ctccccccta cttcatcgcc gtgtcgaaga tgctcccgat
     6541 tggaggaggt gtctactgct tcgctgattc agacggctac tcgacggtca gcgcgacgag
     6601 gtcatcggtg ccgtctgcgc tggcctcagt ggggacgaag accgtgtgtc tcgccaccgc
     6661 gatcggcgaa ccgagataga actgttggtc tcccagagac gtgaacgaca gccatgtaca
     6721 cgtcggttgg ccacagccgt cagcggggta ggacagcacg gcctggtcca cagggaagcc
     6781 cgacgccgga cccttggcga cgaagaccac gtcgcccgac acgaccggcg gcgactcgaa
     6841 tccggacgcg aaattgaccg ccgtccacaa cggatcgcac acggctgccc cacatccgtc
     6901 agctgggaac gccatgaaga cgccgatcgt gttggggtcg ggtgtgctct gcgagccggc
     6961 gtagatgacg ccgttcgcga cggcgagccc accggtcagg tcggcgtcgc cggtgcggta
     7021 cgagcgcacc gggtggcaga agcgcttgcc gcagccggcg acgtcgaacg cgaacaggtg
     7081 gacggggccg ttgggcacgt tggagaaccg ggttcccgac cccatgaaga ccgtgtcgcc
     7141 gaggaccagc ggtgacagcg ttccgtccac ctggccacgc atgtcggccc gccactccgg
     7201 tcgacaggtg gccttcccgc agccgagggt cgcgaacgag aacaaccgtc cactggcgtc
     7261 gtctggtgtg ttgaagaagg cgccgatgaa cacgtggccg tcggcgaccg ccgggatcgt
     7321 gttgaactgg agattcggtc ctgcgatgcc ggtccacagc ggctggcaga cgggatgacc
     7381 gcaaccgtgg gcgtggaagg cgtacacgtg gccgccggcg tcgccgatgt acgcacgccc
     7441 acggaacacg gccaccgacg cgagtgcgga gtcctgcagc ttgccccgcc agaccggagg
     7501 gcaggtccgc cggccgcatc cgtcggtgtg gaacgcgtag aggaagtggt cagcggagcc
     7561 gaccaggacg agtccatcgg tcacgatggc cggggtgccg tggatgccgt tcttggtgac
     7621 gccgcgccac aacggcgtac aggagccggt cgcgcacgcc tggagcgaga acgccgagag
     7681 tacgccgtca gcgccgccga tgaaggcctg tcccgcggcg acggcggctc cgccggtgct
     7741 cgacgccgag gtgccgaagg tggtccgcca ggcctcgtgg agccggccga ccgtctgctg
     7801 ggtgaacgcc ccctcggcca ccgcgtggtt gtgatggact gccctgccgg cgaactgttt
     7861 ccagtccccg ccggccggta ccacgatgcc gacgtgggag gcggcgcggt cgctcgcctc
     7921 acctgccgcg gcgaggctcg acatggcggc cacgaggagc agacagcagg cgacccggac
     7981 acagcggctc atggcgacga cgcctcctcg accgagaagg tcgcgcgggc gtccgcagcg
     8041 gtccgcggcc caccatgctc gcgcgcaccg gcctcaacat tgccggggtg gacactgtcg
     8101 tgtccacccc ggctttccct cgtcagccgc tactgcttga cccagatgtc tcgccaggtc
     8161 ggcatgccgt aggtgttgtc gttgtgcatg ccctcgatct tcgagccgtg catcatcgcc
     8221 accccggcgt accccgtgtt gaacagcggg aagtacttgg tcaggacttc cttgtccagg
     8281 tcgttccagg caccgggctg cttgtccagc ggcatcagct ggatgtcgct gatctgcttg
     8341 tcgaccgcag gctcgttgaa caccgcgtag ttggcgccga gaccctcttc cttgaggttg
     8401 gtcgacttga tcaccggcgg gaaccacgac gagccggtcg gccagtcaga gcaccagccg
     8461 gcggaccgga cgttgatctt ggcgtccggg tcggagcgca ccgtcgacag gtcggccacc
     8521 gtggtggcca ccggctcagg cttgaagcca cccgccgtca aacccttgac gatggcgtcc
     8581 ttgacgtcca ccgataccgg gtcgtcctgt gagtacagga actggatcgg gtagttcatc
     8641 gcaccggcct gttccagcag ctccttcgac ttcgccggat ccgtc
//
LOCUS       DATBFZ010000372         1625 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_4395682, whole genome shotgun sequence.
ACCESSION   DATBFZ010000372 DATBFZ010000000
VERSION     DATBFZ010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4395682"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..585)
                     /locus_tag="VH419_11230"
     CDS             complement(<1..585)
                     /locus_tag="VH419_11230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044230.1"
                     /translation="MKFRLSLRTATDRFTGRPGDANGVERDTGGRHRESREPGSSESK
                     AVVRAERRPSQTAKVTRNLLVFAVVALGLSVPVAYAARHPKRPHHHRHHHGTTTTTRT
                     TTTTTTTTTTGQSTTPQSKPKPTTTGTVTVDGSWTCSGPVNIASLTVTNHGSGDAVKL
                     RGGCTGRIGRIAVTGVQNGDGIKVQGGVHDLTIES"
     gene            735..1100
                     /locus_tag="VH419_11235"
     CDS             735..1100
                     /locus_tag="VH419_11235"
                     /inference="COORDINATES: protein motif:HMM:NF018561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1254 domain-containing protein"
                     /protein_id="HEY7044231.1"
                     /translation="MSHPVPVTPDNFIRAETDRYFGMFVDRGALGSFFHHRDLPPIDL
                     PGVRPNRDTLYSEVVVDLDAGPAMVTLPDPGDRFLSFLETVHALQDALEIAQTSRGTF
                     DVPAWDEASRRRCARHCWS"
     gene            1052..1543
                     /locus_tag="VH419_11240"
     CDS             1052..1543
                     /locus_tag="VH419_11240"
                     /inference="COORDINATES: protein motif:HMM:NF018452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1214 domain-containing protein"
                     /protein_id="HEY7044232.1"
                     /translation="MGRSEPTKVRKALLELSSTLPDLRNGGGRRDEVDPVRHLIATAS
                     GWGLSPDKDAIYLNITPPSNDGSGAYELTVGEVPVDGFWSVSVYDAEGHFVENDRAAY
                     TLNNITAAKNPDGSVTIRFGGDDDQPNCLPIFPGWNYMVRLYRPRPEVLDGRWTFPSL
                     EAA"
BASE COUNT          246 a          568 c          523 g          288 t
ORIGIN      
        1 gctctcgatc gtcagatcgt ggacgccacc ctggaccttg atgccgtccc cgttttgcac
       61 gccggtgacg gcgatgcggc cgattcgtcc cgtgcagccg ccgcgcagct tgaccgcgtc
      121 gccgctgccg tgattcgtga ccgtcagcga ggcgatgttc accgggccgg agcacgtcca
      181 gctgccgtcg acggtgaccg tgcccgtcgt ggtcggcttg ggcttcgact gcggcgtggt
      241 gctctgcccc gtggtggtcg tcgtcgtggt tgtcgtggtg gttctggtgg tcgtcgtggt
      301 gccgtggtgg tggcggtgat gatgcggacg tttgggatgc cgggcggcgt atgccaccgg
      361 gacgctcagg ccgagcgcta cgaccgcgaa caccagcagg ttcctcgtga ccttcgccgt
      421 ctggctcggg cgcctctccg ctcgaacgac ggccttcgac tccgacgagc cgggctcacg
      481 cgactccctg tgtcgccccc ccgtgtcccg ttcgacgccg ttcgcgtcgc cgggccttcc
      541 ggtgaagcgg tccgtggccg tgcgtaggct cagcctgaac ttcatgtgat gtctccttcc
      601 tgggcgcagt tcggggccct gggcgtaggc cccgcggttg ccgcggggcg ctacccgatc
      661 tgaaagttct gaaacctcca ggacgagaag ccgagagcgg aaacgtcgtt attctcggcc
      721 ggcgaacggt cgacgtgtca cacccggttc ccgttactcc cgacaacttc atccgcgccg
      781 agacggacag gtacttcggc atgttcgtcg accgcggcgc gctcggcagc ttcttccatc
      841 atcgcgacct gccgccgatc gaccttccgg gggtccgtcc gaaccgcgac accctctact
      901 ccgaggtcgt ggtcgacctc gatgccggac ctgcgatggt cacgctcccc gacccgggcg
      961 accgcttcct gtccttcctc gaaaccgtcc atgcgctcca ggacgctctc gagatcgccc
     1021 agacgagccg cggaacgttc gacgtgcctg cgtgggacga agcgagccga cgaaggtgcg
     1081 caaggcactg ttggagctga gctcgacgct gcccgacctg cggaacggcg gcggccgccg
     1141 ggacgaggtc gatcccgtcc ggcacctgat cgcgaccgca tccgggtggg ggctgagccc
     1201 cgacaaggac gcgatctacc tcaacatcac gcccccgagt aacgatggca gcggcgcata
     1261 cgagctcacg gtcggcgagg tgccggtgga cggcttctgg tcggtcagcg tctacgacgc
     1321 cgaaggacac ttcgtcgaaa acgatcgtgc cgcgtacacg ctcaacaaca tcaccgcagc
     1381 gaagaacccc gacggatcgg tcaccatccg cttcggcggc gacgacgacc aaccgaactg
     1441 tctcccgatc ttccccggct ggaactacat ggtgcgcctc taccgcccga gaccagaggt
     1501 actcgacggc cgctggacgt tcccgtccct cgaagctgcc tagccgggcc ggcctcacgc
     1561 gtgactgcaa gaaccgatac cggacttccc gcgcagttgt acgacgcgat cggaaccacc
     1621 tacag
//
LOCUS       DATBFZ010000373        13304 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_4724729, whole genome shotgun sequence.
ACCESSION   DATBFZ010000373 DATBFZ010000000
VERSION     DATBFZ010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 13304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13304
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4724729"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..528)
                     /locus_tag="VH419_11245"
     CDS             complement(7..528)
                     /locus_tag="VH419_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008357007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEY7044233.1"
                     /translation="MSLERPIAEDPYAKLPSVPSFTVSSNDVTDGQPLKDDQVAAKGN
                     TSPHLSWSGFPEGTQSFVVTCFDPDAPTPSGFWHWIAVDIPADVTELATDAASSGLPG
                     NGFHVRNDAGEKSFTGAAPPEGDQVHRYYFVVHAVGEDTLGVDDTVSPAVVSFNLAFK
                     TLARAQVVGTYQH"
     gene            655..1890
                     /gene="cobA"
                     /locus_tag="VH419_11250"
     CDS             655..1890
                     /gene="cobA"
                     /locus_tag="VH419_11250"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009078879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEY7044234.1"
                     /translation="MVDAEHHLYPAGLRLTGRRVVVVGGGHVAQRRLPSLIAAGAAIE
                     VISPVTTPAVEGHARAGDIVWHSRRYAPGDLADAWYVVCATDDRQVNEAVSLEAETRR
                     VFCVRSDDATAATAWTPAVGRHGPLTVAVLGSRDPRRSASVRDEILNRLRSGEIVAHK
                     DRVRRPGVTLVGGGPGDPDLITVAGRRALQEADVVVADRLAPRDLLAELAPDVELVDA
                     TKLPRGRAASQEAINELMVGRARAGRNVVRLKGGDPYVFGRGFEEAQACAAAGVEFHV
                     IPGITSAVAVPGLGGIPLTHRGVAHEFTVVSGHLRPGDETSLVDWNALGRMQGTLVLL
                     MAVDNLSWITRALVDSGRDPGTPAAVVQDGSLTSQRAVFSTVAEIASDVVREGLRPPA
                     IVVIGEVVALARSLGAVPA"
     gene            1927..2772
                     /locus_tag="VH419_11255"
     CDS             1927..2772
                     /locus_tag="VH419_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002520242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEY7044235.1"
                     /translation="MAELIRVDDPADPRLADYVSLRDVQLRRHLEAEHGFFIAEGEKV
                     VRRAASAGYPVRSFLMAERWLDGLRDILDSAADAPCYVVTEALAEQVTGFHVHRGALA
                     ALARTAVPPVEEVLARARRIVLLEDIVDHTNVGAIFRCAAAFGMDAVLLSPRCADPLY
                     RRAVKVAMGAVFSVPWTRLDDWYDAMPMLAGRGFQTIALSLSPDSTPLTEVQVAQRTA
                     LVMGAEGHGLTHRWQQGASIRATIPMANGIDSLNVAAAAAVACHWLRFADSWDRAAAG
                     PPSAR"
     gene            2974..4449
                     /locus_tag="VH419_11260"
     CDS             2974..4449
                     /locus_tag="VH419_11260"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HEY7044236.1"
                     /translation="MGGNRSGVAATARRLHETLATLHAAPKPRQALAELCRGIRDTLD
                     ARTIVFHLVEPGVDGDIVEAVAYAGDESAAQLLGVRAPVSDWNHLLAKSPSSDGVHHC
                     IDPRAFLDVVVHEPVDTEPEMHGDDERWGALNLHVAPVTVRDELAGLITATYPGGSAR
                     PDPAVVPLLALFAAQAGVAFHHHLFVRRSSDDHLALRLSEERFRLAFDNAPIGMAEFV
                     EGPSGLEIARINQAAGQMFGVNVFGAQHEPIDRVLTVVDGEPLGALMSRLLEDDRRGL
                     RMEVPFVGADGGEFWGLVEAASLMDGGGRPGVLCQIVDITQSKADEQELTIRAQHDPL
                     TGLPNRLTVLDHLQEVVREARETGESGALLFCDLDHFKHVNDEHGHLVGDDALSAIAT
                     RLHQTVRKSDVAGRFGGDEFVVVAFPITQDEAQSLGERIQLAMSEPIVLDGAALRVGL
                     SVGIAMVTGAVEASEVLRRADAAMYAVRSHQRRPSFVVDTA"
     gene            complement(4474..4995)
                     /gene="def"
                     /locus_tag="VH419_11265"
     CDS             complement(4474..4995)
                     /gene="def"
                     /locus_tag="VH419_11265"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEY7044237.1"
                     /translation="MTRWGEPVMHAPCAPVTSYDDELRTLVADLVATMYAADGVGLAA
                     NQIGVSLSVFVFDCPDEDNAPVRGVVCNPKLVVPEGKDRVLDDAEEGCLSLPGAFVTC
                     ARPDHATVYGQDQFGEPVTYSGTGLLARCLQHESDHLVGTVFGDRLPTRQRKKLFKEA
                     DKHAADFPPGWPA"
     gene            complement(5066..5848)
                     /locus_tag="VH419_11270"
     CDS             complement(5066..5848)
                     /locus_tag="VH419_11270"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HEY7044238.1"
                     /translation="MSEQLPVIYLSHGAPPLADDAVWTRQLAQWSADMPRPTAVLVVS
                     AHWEEAPLTLGATTTVPLVYDFWGFPERYYQVQYPAPGAPELAEKVRGLLRSPKHPIH
                     DAPNRGLDHGAYVPLVEMFPSADVPTLQISMPTLDPATLFDIGRKLAPLRDEGVLILG
                     SGFTTHNLSLISMSRPAAAPAPEWSREFDDWAARTIAAGDVDAVIDFLHKAPAAHVAH
                     PRTEHFAPLFVSLGASVDGLREGRSVIDGFWYGLSKRSWQFN"
     gene            complement(5927..6856)
                     /locus_tag="VH419_11275"
     CDS             complement(5927..6856)
                     /locus_tag="VH419_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020392663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03086 family metal-binding protein"
                     /protein_id="HEY7044239.1"
                     /translation="MTETADVAELYRRSVQGWKTKLAAITSEQWDAPTPCTDWSVRDL
                     VNHVVGEELWTPPLMHGQTIEQVGDRFDGDLLGDDPLSSAEAASDEALSAAPEGSLHG
                     HKVHLSYGEEDAEEYLRQLIADHLIHGWDLAAAIGSDTRLDADLVSAVAAWFKDREEL
                     YRSAGAVGPRADGGGDAQSDLLAAAGRRADWNPLQAPLAAFSAAFGSGDIDAIMALMT
                     DDCVFESTSPAPDGGRYDGAGAVRQVWEDLFGGTPDAAFSEEESFVAGDRGVLRWRFT
                     WTAPDGGQGHVRGVDVLRFRDGKVCEKLSYVKG"
     gene            complement(7020..7712)
                     /locus_tag="VH419_11280"
     CDS             complement(7020..7712)
                     /locus_tag="VH419_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009079607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEY7044240.1"
                     /translation="MTADHALVDAVTEGLRSLADPAAAPQMQRYMKSAMPYLGVKMPV
                     VRQLTRRLYAEHVLADRATWEDTVRALFDQATYREERYAALMLVGHRRYAYYLDAAAL
                     PLLEHVIAAGAWWDIVDDVSHRVGDALRTDRRATEAVVRGWIGADDIWLRRAAIICQL
                     GHRTDTDVTLLQDAILPAMHEREFFLRKAIGWALREYAKTDPDWVRRFVATHQEELSA
                     LSRREALRNLPG"
     gene            complement(7709..8863)
                     /gene="glgA"
                     /locus_tag="VH419_11285"
     CDS             complement(7709..8863)
                     /gene="glgA"
                     /locus_tag="VH419_11285"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020580239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HEY7044241.1"
                     /translation="MRVGLVTREFPPEVYGGAGVHVDFLSRHLRDLVDLTVVCTGAER
                     DGAIAIPEQDGRFPTANSALHILSADTAMTAACEGMDLVHSHTWYANMAGHLSSLLYD
                     VPHVVTAHSLEPQRPWKAEQLGGGYRLSSWAEKTAYAGAAAIVAVSEGMAADVKAAYP
                     DVDPDRIHVIYNGIDADFYRPDSGTDILRELGVDPARPYVTFVGRITRQKGVPHLLRA
                     GLQLDPSVQLVLLAGAADTAELKEETDVAIARLREARDGVIVVNEMLPRESVRQVLTH
                     ALAFVCPSVYEPLGIVNLEAMACETAVVASAVGGIPEVVVHGETGLLVDYTPEDVAGF
                     EAALAAAINRLAADPSMAEQMGKAGRARAVESFDWARIAEQTVALYRTLT"
     gene            8955..10202
                     /gene="glgC"
                     /locus_tag="VH419_11290"
     CDS             8955..10202
                     /gene="glgC"
                     /locus_tag="VH419_11290"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015583080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEY7044242.1"
                     /translation="MVMARPKVLGIVLAGGEGKRLMPLTLDRAKPAVPFGGSYRLIDF
                     ALSNLVNAGYLRLCVLTQYKSHSLDRHISVTWRMSTMLGNYVTPVPAQQRRGKQWFLG
                     SADAMYQSMNLIIDEDPDIIVVFGADHVYRMDASQMVAAHLESGASCSVAGIRVPRKD
                     ADQFGVIKTADDGVSIAEFLEKPADPPGLVDSPDEAFASMGNYVFTRDALCEALEKDA
                     ANSESRHDMGGDIIPMFVDRKEAAVYDFKHNDVPGSLPTDRDYWRDVGTVASYHEAHL
                     DLVSVEPVFNLYNNQWPIFTSSPQLPGAKFVEDATADDAIVCAGSIVSGAAVRSSVIG
                     TDVYVATGAVVNRSVLMDGVKIGPGANVSNAIIDKNVVVPDGYSIGLDPKADRAAGFT
                     VTEEGITVLGKGHRIPGKYTGAK"
     gene            10243..11640
                     /locus_tag="VH419_11295"
     CDS             10243..11640
                     /locus_tag="VH419_11295"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEY7044243.1"
                     /translation="MSIEGPLPPSVAAGNPVTAEVGAQILDEGGCAADAAVAMVLTAC
                     VAETVFTGLAGGGFAIYHDAATSETTCLDFFVCVPGLAGRRETRAQEIAIDFGGQLVP
                     YAVGPATVAVPGVPAGMAALHERWGRLPWRQVVEPAAAHAGRGVSFAPLHSKVLATVA
                     PAMLLGEGGRVYARDGKVLPGGARLFHPGLEAAMRALADEGAEAFYTGSVAESIVTAI
                     GMNGDLSYADLAAYQVEESTPRQVELGPAIVRARGDDLDDLLGTIGALVLDPDPEVMA
                     TRLVDALRMPAHRGDTTNAAVVDHDRNICAVTTSLGLASGVWLADYGLHLNSMLGESE
                     LIRGTERPGRRLGSMMSPLIVFQDADPMLVAGAAGGSRIRSALLQVVVNVVHGGLGVA
                     DAIVAPRLNPVPGKVHVEPGFAPEVLQALSTRDEVVAWPGLDAYFGGVSAIGTTGPGA
                     DPRRGGDVRRLAEVG"
     gene            11799..12581
                     /locus_tag="VH419_11300"
     CDS             11799..12581
                     /locus_tag="VH419_11300"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY7044244.1"
                     /translation="MSNTKRATRPTPLPRKGTSQATKIAIAVIALLCVAAVIAIPILL
                     NGGDDDSSAQVSSHPNPTSVDDVSCDKPPATPSNPKQFSTPPPASLAENTTWNATLHT
                     TCGDITVQLDGKAAPQTVASFIHLAREGYWNDGPCHRVTTEESSIHVLQCGDPTGTGS
                     GSPGYGFGIENAPASGHYPRGTLAMARSSDPNSNGGQFFLVYKDTQLPTQDGGYSIFG
                     KVTGGLQIVDAIAKQGTDGANGDGAPSQPISILSVDVEKANS"
     gene            complement(12585..>13304)
                     /locus_tag="VH419_11305"
     CDS             complement(12585..>13304)
                     /locus_tag="VH419_11305"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY7044245.1"
                     /translation="GRRCAASSTDDNAARRRLDRRNGKRLPTRDTKLVGVDAEQWDEK
                     YAANELVWGAEPNRFVRAQCERLPVGRAVDLACGEGRNALWLARLGWKATGVDFSNVA
                     IERARRLTTREAPAVAGRLTWRVGDVTAEPIKRGGYDLALISYLHLAPEANAAVIRNA
                     GYAVAPGGHLVIVGHDRRNLSEGVGGPPDAARLYDGDEIRAVLADVPSLVVELAEVVK
                     RETPDGVALDTLVRARRPAAA"
BASE COUNT         1992 a         4566 c         4660 g         2086 t
ORIGIN      
        1 ccgcgatcag tgctggtagg tcccgacgac ctgcgcgcgg gcgagcgtct tgaaggccag
       61 gttgaacgac acgaccgccg gggagacggt gtcgtcgacg ccgagtgtgt cctcgccgac
      121 cgcgtgcacg acgaagtagt accggtgcac ctggtcgccc tccggcggag cggcaccggt
      181 gaagctcttc tcgcctgcgt cgttgcgtac atggaacccg ttaccgggca gaccgctgct
      241 cgccgcatcc gtcgccagct cggtgacgtc ggcggggatg tcgaccgcga tccagtgcca
      301 gaaccctgat ggtgtcggtg cgtccgggtc gaagcaggtc acgacgaaac tctgcgtccc
      361 ctcgggaaag ccactccatg acaggtgggg tgaggtgttc cccttggcag cgacctggtc
      421 gtccttgagc ggctgaccgt cggtcacgtc gttgctcgac acggtgaagc tcggcaccga
      481 cggaagcttg gcgtacgggt cttcggcaat gggtcgttcg aggctcatgg gtccatcttg
      541 gcccgtcgct ggccggcggc aagtggtctg gtgctcccgc acaccatcgg gggagcactc
      601 ccggctgatg agacgtacgt cggcgtcgcg ttcgtgccgc ttagcgtaga acgaatggtc
      661 gacgccgagc accacctcta cccggcaggg ctacgcctga ccgggcgccg ggtggtcgtc
      721 gtcggcggcg ggcacgtcgc gcaacgccgc ctcccgtcac tcatcgccgc tggcgcggcg
      781 atcgaggtca tctcgcccgt cacgactccg gcggtcgaag gccatgctcg cgccggcgac
      841 atcgtctggc actcgcgccg ctacgcgccc ggcgatctcg cggacgcctg gtacgtcgtg
      901 tgtgcgaccg atgaccggca ggtcaacgag gcggtgtcgc tcgaggccga gacgcgccgg
      961 gtcttctgtg tgcggtccga cgacgccacg gcggccacgg cgtggactcc ggcggtgggt
     1021 cgccacggcc cgctgaccgt ggccgtgctc gggagccgag acccccggcg gtccgcctcc
     1081 gtgcgcgacg agatcctcaa tcggttgcgc agcggcgaga tcgtcgccca caaggaccgc
     1141 gtacgccgcc ccggggtcac ccttgtcggc ggtgggcccg gcgaccccga cctgatcacg
     1201 gtggcgggtc gacgtgccct gcaggaggcc gacgtcgtcg tcgctgaccg gctggccccg
     1261 cgggacctcc tggccgagct cgcgcccgac gtcgagctcg tcgacgccac gaagctcccg
     1321 cgcggacgag cagccagtca ggaggccatc aacgagctga tggtcgggcg agcgagagca
     1381 ggccgcaacg ttgtgcgcct gaagggcggc gacccgtacg tcttcggccg tggcttcgag
     1441 gaggcgcagg cttgcgcggc agcgggcgtc gagttccacg tgatccccgg catcaccagc
     1501 gcggtggcgg ttccggggct cggcggcata ccgctgacac accggggagt ggctcacgag
     1561 ttcacggtcg tctccggaca tctccggccc ggcgacgaga catcgctggt cgactggaac
     1621 gcgctcggcc gcatgcaggg gacgctcgtg ctgctgatgg ccgtcgacaa cctgtcgtgg
     1681 atcacccgcg ctctggtcga ttccggccgt gatcccggca caccggcggc tgtcgtccag
     1741 gacggctccc tcacgtccca gcgtgcggtg ttctcaacgg tcgcggagat cgcgtccgac
     1801 gtcgtacggg aagggctgcg gccgcccgcg atcgtcgtca tcggcgaggt ggtggcgttg
     1861 gcgcgctccc tgggagccgt gcccgcctga cccggaccgc cggctccggg ctgggatcat
     1921 gtcgacatgg cggagctgat ccgggtcgac gatcctgcgg atccacggct cgccgactac
     1981 gtctcgctgc gtgacgtgca gctgcgtcgg cacctcgagg ccgagcacgg gttcttcatc
     2041 gccgaaggcg agaaggtcgt tcgccgggcg gcgtcggcgg gttaccccgt gcggtcgttc
     2101 ctgatggccg agcgatggct cgatggactc cgcgacatcc tcgactcggc tgccgacgcg
     2161 ccctgctacg tcgtgacgga ggccctggcc gagcaggtga ccgggttcca cgtccaccgc
     2221 ggcgccctgg ctgcactcgc gcgcaccgca gtgcccccgg tcgaggaggt gctcgcgaga
     2281 gcccgccgga tcgtactgct cgaggacatc gtcgaccaca ccaacgtcgg agcgatcttc
     2341 cgctgcgcag ctgcgttcgg catggacgcg gtcctgctct ctccgcggtg tgccgacccc
     2401 ctgtaccggc gggcggtgaa ggtcgccatg ggtgcggtgt tctccgtccc atggacacgt
     2461 ctcgacgact ggtacgacgc gatgccgatg ctcgccggcc gtggcttcca gacgattgcc
     2521 ctgtcgttgt caccggacag cacgccgttg acagaggtcc aggtggcgca gcgcaccgca
     2581 ctcgtcatgg gggccgaggg gcacggactc actcatcgat ggcagcaggg cgcgagcatc
     2641 cgggcaacga tcccgatggc gaacgggatc gactccctca atgtggctgc ggccgcggct
     2701 gtggcctgtc actggctgcg gttcgccgac tcgtgggatc gcgccgcggc ggggccgccg
     2761 agcgcacggt gagtccccgc ggaagttccg gatcgccgga cgacgggaac ggtgtgcgcc
     2821 tatcctgggc tccgagggaa cggggtcttt cgtcgcgttg gctcgccgat gtaccgacga
     2881 gtcccgagtt ggaggatgtc ggctcaccga atgcctggct gccggcgcga cgccttcttc
     2941 gtggtcatac ggactgatgt gcaggggggc acagtgggag ggaaccgctc gggagtggcc
     3001 gcgaccgcgc gccggctcca cgagacgttg gcgaccctgc acgccgcccc gaagccgcga
     3061 caggccctgg ctgagctgtg tcgtggcatc cgtgacaccc tggacgcccg gacgatcgtc
     3121 ttccacctgg tcgaacctgg tgtggacggc gacatcgtcg aggcagtcgc gtacgccggc
     3181 gacgagtccg ccgctcagct gctcggtgtc cgggcccccg tctcggactg gaaccacctg
     3241 ctggccaagt cgccgtcgag cgacggtgtc caccactgca tcgacccccg ggcgttcctc
     3301 gacgtggtcg ttcacgagcc ggtcgacacc gagccggaga tgcacggtga cgacgagcga
     3361 tggggggcgc tgaacctcca tgtcgcgccg gtcacggtcc gtgacgagct cgccggcctg
     3421 atcaccgcca cctacccggg cggatctgct cggccggacc cagccgtcgt accgctactg
     3481 gcactgttcg ccgcccaagc cggcgtggcc ttccaccacc atctgttcgt gcggcgctcg
     3541 tccgacgacc acctcgcgct ccggctgagc gaagagcggt tccggctggc gttcgacaac
     3601 gccccgatcg ggatggcgga gttcgtcgag gggccgtccg gactcgagat cgcgcgcatc
     3661 aaccaggcgg ccggccagat gttcggcgtc aacgtcttcg gcgcccagca cgagcccatc
     3721 gaccgcgtgc tgaccgtggt cgacggcgaa ccgctcggtg cgctcatgtc caggctgctc
     3781 gaggacgacc ggcgtgggct gcggatggag gtcccgttcg tcggcgcgga cggtggcgag
     3841 ttctgggggc ttgtcgaggc ggcgtcgctg atggacggtg gcgggcggcc cggcgtgctg
     3901 tgccagatcg tcgacatcac gcagtcgaag gcggacgagc aggagctcac gatccgcgct
     3961 cagcacgatc cgctgaccgg gctgccgaat cggctcaccg tgctcgacca cctccaggag
     4021 gtcgtccgcg aggctcgcga gaccggggag tccggtgcgc tgctgttctg cgacctcgac
     4081 cacttcaagc acgtcaacga cgagcacgga cacctcgtcg gtgacgatgc actttcggca
     4141 atcgccaccc ggcttcacca gactgtccgg aagtccgacg tcgcgggccg gttcggcggc
     4201 gacgagttcg tggtggtggc cttcccgatc acccaggacg aggcgcagtc gctgggcgag
     4261 cggatccagt tggcgatgtc ggagccgatc gtgctcgacg gcgccgcgct gcgggtcggg
     4321 ttgagcgtcg gcatcgcgat ggtgacgggc gcggtcgagg cctcggaggt gctccgccga
     4381 gcggacgccg cgatgtacgc cgtacggtcc caccagcgcc gacccagctt cgtcgtcgac
     4441 accgcctgac cgaccggggc tcccgccccg gggctaggcg ggccagccgg gcgggaagtc
     4501 ggcggcgtgc ttgtcggcct ccttgaagag cttcttgcgc tgccgggtcg gcagccggtc
     4561 gccgaagacg gttcccacga ggtggtcgct ctcgtgctgc agacagcggg cgagcaagcc
     4621 ggtcccggag tacgtcaccg gctccccgaa ctggtcctgg ccgtacaccg ttgcgtggtc
     4681 gggcctcgcg caggtgacga aggcgccagg taacgagaga cagccttcct ctgcatcgtc
     4741 gagcacgcgg tccttgcctt cgggcacgac gagcttcgga ttgcagacga cgccgcggac
     4801 cggcgcgttg tcctcgtccg ggcagtcgaa cacgaagacg ctgagcgata ccccgatctg
     4861 gttggcagcc agcccgactc cgtctgcggc gtacatcgtg gccacgaggt cggcgaccag
     4921 cgtgcgcagc tcgtcgtcgt acgacgtgac gggcgcgcac ggcgcgtgca tgacaggctc
     4981 gccccaacgg gtcatcgcac gcacggtgcc gccgctcgga agctcggtca cgcgcgcgac
     5041 gctatcgcag ccggtggcca gcaggtcagt tgaactgcca ggagcgtttg gacagcccgt
     5101 accagaaccc gtcgatcacc gaacgcccct cgcgcagccc gtcgacactc gcgcccaggc
     5161 tcacgaacag cggcgcgaag tgctccgtcc tcggatgtgc gacgtgcgcc gccggagcct
     5221 tgtggaggaa gtcgatgacc gcgtcgacgt caccggccgc gatcgtccgc gccgcccagt
     5281 cgtcgaactc gcgcgaccac tcgggcgcgg gtgcggcggc cggccggctc atggagatca
     5341 ggctcaggtt gtgcgtcgtg aacccgctcc cgaggatcag cacaccctcg tcacgcagcg
     5401 gcgccagctt ccgtccgatg tcgaacagtg tggccgggtc gagtgtcggc atcgagatct
     5461 ggagcgtggg tacgtcggcg ctcgggaaca tctcgacgag cgggacgtag gcgccgtggt
     5521 cgaggccgcg gttcggcgcg tcgtggatcg ggtgcttggg tgagcgcaac agcccgcgca
     5581 ccttctcggc cagctcgggg gccccgggtg ctgggtactg cacctggtag tagcgctcgg
     5641 ggaagcccca gaagtcgtac acgagcggga ccgtcgtcgt tgcgccgagg gtgagcggag
     5701 cctcctccca gtgcgcggac accaccagga cggcggtcgg cctcggcatg tccgccgacc
     5761 actgtgcgag ctgccgcgtc cacaccgcat cgtcggccag cgggggagca ccgtggctca
     5821 ggtagatcac cggcagctgc tcactcatcg gcactccagc ccactctcga gtcgtccgac
     5881 cccgtgcaca catcttcctc caacgccact cgggtgagtc gtgtggtcag cctttgacgt
     5941 aggagagctt ctcgcagacc ttgccgtcgc ggaaccggag cacgtccacc ccccgcacgt
     6001 gtccttggcc gccgtccggg gccgtccagg tgaatcgcca acggagtacg ccgcggtctc
     6061 ctgccacgaa ggactcctcc tcggagaacg ccgcgtctgg cgtccccccg aacaggtcct
     6121 cccacacctg gcgtacggcg ccggctccgt cgtagcggcc gccgtccggc gcgggcgacg
     6181 tggactcgaa gacgcagtcg tcggtcatca gagccatgat cgcgtcgatg tcgccactgc
     6241 cgaacgcggc ggagaaggcc gccaggggcg cctggagcgg gttccagtcc gcgcggcgac
     6301 ccgcagccgc cagcaggtcc gactgcgcgt cgccaccacc gtcggcccgc ggaccgacag
     6361 caccggcaga gcggtagagc tcctcgcggt ccttgaacca cgcagccact gcgctcacca
     6421 ggtcggcgtc cagccgggtg tcggagccga tcgccgcggc caggtcccag ccatggatga
     6481 ggtggtcggc gatgagctga cggaggtact cctcggcgtc ctcttcaccg taggagaggt
     6541 ggaccttgtg gccgtgcagc gacccttctg gtgccgcgga caacgcctcg tcggaggccg
     6601 cctcggctga cgagagcggg tcgtcgccca acagatcacc gtcgaaccgg tcaccgacct
     6661 gctcgatggt ctgcccgtgc atcagcgggg gcgtccagag ctcctccccg acgacgtggt
     6721 tgaccaggtc gcgtaccgac cagtcggtgc acggcgtcgg cgcgtcccac tgctcggacg
     6781 tgatcgcggc cagtttggtc ttccatccct ggacgctccg gcggtacagc tcggcgacat
     6841 cggcggtctc ggtcatcagg cggtccctcc tcagctcccg gactccctgg cccgagcgtg
     6901 ctccacgatc acgcgttcgt ccaccatggg cggtccggcg gtcgagcttg tcgagacctc
     6961 gcccgtgatc tcgacgggct cgatcagcgg gctgtcgacg ggctcgatca gcgggcttct
     7021 cagccgggga ggttccgcag ggcttcgcgt ctggacaggg ccgacagctc ctcctggtgc
     7081 gtggcgacga agcggcgtac ccaatccggg tctgtcttgg cgtactcacg caacgcccag
     7141 ccgatggcct tgcgcaggaa gaactcgcgt tcgtgcatgg ccggcaggat cgcgtcctgg
     7201 agcagcgtga cgtcggtgtc ggtgcgatgc ccgagctggc agatgatcgc agcccggcgc
     7261 agccagatgt cgtccgcgcc gatccaaccg cgcaccaccg cctcggtcgc acgacggtcg
     7321 gtccggaggg cgtcgccgac acgatgcgac acgtcgtcga cgatgtccca ccaggcgccg
     7381 gcagcgatca catgctcgag cagcggcagg gctgccgcgt ccaggtagta cgcgtagcga
     7441 cggtgaccca ccaacatcag cgcggcatag cgctcctctc ggtacgtcgc ctggtcgaac
     7501 agcgcgcgaa cggtgtcctc ccacgtggct cggtccgcaa gcacgtgctc ggcgtagagc
     7561 cgccgggtca gctgccggac caccggcatc ttcaccccga ggtacggcat ggcggacttc
     7621 atgtagcgct gcatctgcgg ggcggcggcc ggatcggcga gcgaccgcag cccctcggtg
     7681 acggcgtcga ccagcgcgtg gtcggctgtc acgtgagagt gcggtacagc gccaccgtct
     7741 gctcggcgat ccgcgcccag tcgaaggact ccaccgcacg ggcccggccg gccttgccca
     7801 tctgctcggc catcgaaggg tcggcggcga ggcggttgat cgcggccgcg agggcagctt
     7861 cgaatccggc gacatcctcg ggcgtgtagt cgacgaggag accggtctcg ccatggacga
     7921 cgacctccgg aatgccaccg accgcacttg cgacgacggc cgtctcgcag gccatcgcct
     7981 cgaggttcac gatgccgagc ggctcgtaga cggacgggca gacgaacgcg agtgcgtgcg
     8041 tgaggacctg ccgcacactc tcgcgcggca gcatctcgtt cacgacgatg acgccgtccc
     8101 gggcctcgcg cagccgcgcg atcgcaacgt cggtctcctc cttgagctcg gccgtgtcag
     8161 ctgccccagc gagcagcacc agctgcaccg acgggtcgag ctggaggccg gcgcggagca
     8221 gatgcggcac gcccttctgc cgggtgatcc ggccgacgaa cgtgacgtac ggccgcgccg
     8281 ggtcgacgcc cagctcgcgg aggatgtcgg tgccggagtc gggtcgatag aagtccgcgt
     8341 cgatgccgtt gtagatgacg tggatgcggt cgggatcgac gtcggggtac gccgctttca
     8401 cgtccgccgc catcccctca ctcaccgcga cgattgcggc agccccggcg tacgccgtct
     8461 tctccgccca ggacgagagc cgatatccac cgccgagctg ctcggccttc cacggacgct
     8521 gcggctccag cgagtgcgct gtcacgacgt gcgggacgtc gtacagcagt gagctcaggt
     8581 ggcccgccat gttggcgtac caggtgtgcg agtgcacgag gtccatcccc tcgcacgcgg
     8641 ccgtcatggc ggtgtcggcc gagaggatgt gcagcgcgga gttcgccgtc gggaaacgtc
     8701 cgtcctgttc ggggatggcg atcgcgccgt cacgctcggc gccggtgcac accacggtca
     8761 ggtccaccag gtcgcgcagg tgccgcgaca agaagtcgac gtgcacgccc gcgccgccgt
     8821 acacctcggg tgggaactcg cgagtgacca gtccgacgcg catgcctgga accttagacg
     8881 agcgccacag atggtgccgg gacgtctcag gccaggtcca ggtggcggcg cgcagcgcgc
     8941 tcgggcacta gtgtctggtc atggccaggc cgaaggtgct cgggatcgtc ctcgctggtg
     9001 gagaaggcaa acgactgatg ccgctgacgc tggaccgggc aaaaccggcc gtcccgttcg
     9061 gcgggagcta tcggctgatc gacttcgccc tgtcgaacct cgtcaatgcg ggttacctgc
     9121 ggctgtgcgt gctcacgcag tacaagtctc actcgctgga ccggcacatc tcggtgacgt
     9181 ggcggatgtc gacgatgttg ggcaactacg tgacgccggt accggcgcag cagcggcgcg
     9241 gcaagcagtg gttcctcggc agcgcggacg cgatgtacca gtcgatgaac ctgatcatcg
     9301 acgaggatcc ggacatcatc gtggtgttcg gcgccgacca cgtgtaccgc atggatgcct
     9361 cgcagatggt cgccgcgcac ctcgagtccg gcgcgtcctg ttcggtcgcc ggtatccgcg
     9421 tgccacgcaa ggatgcggat cagttcggcg tcatcaagac cgccgacgat ggagtcagca
     9481 tcgccgagtt cctcgagaaa ccggccgacc cgccgggcct cgtcgacagc ccggacgagg
     9541 cgttcgcgtc gatgggaaac tacgtcttca cgagggacgc gctctgcgag gcgctcgaga
     9601 aggacgcggc caacagcgag tcgcggcacg acatgggtgg cgacatcatc ccgatgttcg
     9661 tggaccgcaa ggaggcggcg gtctacgact tcaagcacaa cgacgtaccc ggctcgttgc
     9721 cgaccgatcg tgactactgg cgggatgtgg ggacggtggc gtcgtaccac gaggcacatc
     9781 tcgaccttgt ctccgtcgag ccggtgttca acctctacaa caaccagtgg ccgatcttca
     9841 cgtccagccc ccagctgccc ggcgcgaagt tcgtcgagga cgccacggcc gacgacgcca
     9901 tcgtgtgtgc cggttccatc gtgtcagggg cggcggtccg gtcatcggtg atcggcacgg
     9961 acgtgtacgt cgccaccggc gcggtcgtca accgcagcgt cctcatggac ggagtcaaga
    10021 tcgggccggg tgccaatgtg agcaacgcga tcatcgacaa gaacgtcgtc gtcccggacg
    10081 gttacagcat cgggctcgac ccgaaggctg accgcgctgc cggcttcacg gtgaccgagg
    10141 agggcatcac cgtcctcggg aagggccatc ggatccccgg caagtacacc ggcgccaaat
    10201 gagctcgctg gtctcgtccc cggcagtggc ggtcaggtgg ccgtgagcat cgaaggaccg
    10261 ctgccgccgt ccgtcgccgc gggcaacccg gtgacagccg aggtcggcgc gcagattctc
    10321 gatgaaggtg gctgcgccgc ggacgcggcg gtcgcgatgg tgctcaccgc ctgtgtcgcc
    10381 gagacggtgt tcaccggtct ggccggcgga ggcttcgcga tctaccacga tgctgcgacg
    10441 tcggagacga cctgcctcga cttcttcgtc tgcgtccccg ggctggccgg ccgccgcgag
    10501 acgcgcgcgc aggagattgc gatcgacttc ggcgggcagc tcgtgccgta cgccgtgggg
    10561 ccggcgacgg tcgccgtgcc gggtgtgccg gcgggaatgg cggccctgca cgaacggtgg
    10621 gggcggcttc catggcgaca ggttgtcgag ccggctgccg cccatgccgg ccgcggcgtg
    10681 tcgttcgcac ccctgcattc gaaggtgctg gcgacggttg ctccggcgat gctgctgggc
    10741 gagggcggcc gggtgtacgc ccgcgacggg aaggtgcttc cgggtggcgc caggctcttc
    10801 catcccggac tggaggcggc gatgcgggcg ctcgccgacg agggcgccga ggcgttctac
    10861 accggaagtg tcgcggagtc gatcgtcacg gcaatcggga tgaacggaga tctcagctac
    10921 gcggacctcg cggcgtacca ggtcgaagag tccacgccac gacaggtcga gctgggaccg
    10981 gcgatcgtgc gggcgcgcgg agacgacctg gacgacctgc tggggaccat cggcgcgttg
    11041 gtgctggacc ccgatcccga ggtcatggcg acgcggctgg tcgacgcact gcggatgccg
    11101 gctcaccggg gagacaccac caacgcagcc gtggtcgatc acgaccgcaa catctgcgcg
    11161 gtgacgacaa gtcttggcct ggcgtcgggg gtgtggctcg ctgactacgg gctgcacctg
    11221 aactcgatgc tcggtgagag tgagctcatt cgcgggaccg agcgtccggg acgacggctg
    11281 gggtcgatga tgtccccgct catcgtgttc caggacgccg acccgatgct ggtggcggga
    11341 gcggccggcg gcagccggat ccgctcggcg ctgcttcagg tggtcgtgaa cgtcgtccac
    11401 ggggggctcg gcgtggccga cgccatcgtg gctccccgtc tgaacccggt gccagggaag
    11461 gtgcacgttg agcccggctt cgcgcccgag gtcctgcagg cgctctccac gcgcgacgag
    11521 gtggtcgcgt ggcccggcct ggacgcgtac ttcggcgggg tgtcagcgat cggcacgacc
    11581 ggtccaggag cagacccgcg ccgcggtggc gacgtacgac gcctcgccga agtcggctga
    11641 tcgagggtgt cgagatcgag ggcggggtct cgacaagctc gaccgccgga tcggcgtggc
    11701 ccgctgatcg agcccgtcga gatcgagggt tgtggctcta ccagcggatc gctcggcctc
    11761 tcatctgcgt ctcaacatgg tcacctaggg tgtcgcgcat gtcgaacacc aagcgggcca
    11821 ctcggccgac gccgcttccc cggaagggaa cgtcccaggc gaccaagatc gccatcgccg
    11881 tcatcgcgct gctctgtgtc gcggcagtga tcgcgatccc gatcctcctc aacggaggcg
    11941 acgacgactc gagcgcgcag gtgtcgtcac atcccaaccc gaccagcgtc gacgacgtca
    12001 gctgtgacaa gccaccggcg acaccgtcaa acccgaagca gttctcgacg cctccacccg
    12061 cgagcctcgc ggagaacacc acgtggaacg ccaccctgca cacgacgtgc ggggacatca
    12121 ccgtccagct cgacggcaag gcagcgccgc agaccgtggc gtcgttcatc caccttgccc
    12181 gtgagggcta ctggaacgac ggaccctgcc accgcgtgac gacggaggag tcgtcgattc
    12241 acgtcctgca gtgtggcgac ccgaccggca cgggcagcgg ctcaccgggg tacggcttcg
    12301 gcatcgagaa cgccccggca tccggtcact acccgcgcgg caccctggcg atggcacgaa
    12361 gcagcgaccc gaacagcaac ggcggccagt tcttcctcgt ctacaaggac acccagctgc
    12421 ccacccagga cggtgggtac tcgatcttcg gcaaggtgac cggcggcctg cagatcgtcg
    12481 acgcgatcgc caagcaaggt acggacgggg ccaacggcga cggcgcacct tcccagccga
    12541 tcagcatcct gtccgtcgac gtggagaagg cgaacagctg agcgtcacgc cgcggcaggg
    12601 cgtcgagctc gtacgagggt gtcgagggcg acgccgtcag gcgtctctcg cttgacgacc
    12661 tccgcgagct cgacgacgag actgggtacg tcggcgagca ccgcgcggat ctcgtcgccg
    12721 tcgtagagcc gggcagcgtc cggcgggccg ccgacgccct cgctgaggtt gcgccgatcg
    12781 tgaccgacga tcaccaggtg tccaccaggt gccacggcgt acccggcgtt tcggatgacg
    12841 gccgcgttcg cctctggcgc gaggtggaga tagctgatca gggccaggtc gtagccgcca
    12901 cgcttgatgg gttcggcggt gacgtcgccg acgcgccagg tcagccgccc tgccaccgcc
    12961 ggcgcctcgc gcgtcgtgag ccgacgagcg cgctcgatcg ccacgttgga gaagtcgaca
    13021 ccggtcgcct tccacccgag tcgggccagc cacagcgcgt tgcgtccttc tccacaggcg
    13081 aggtcgacgg ctcggcccac cggaagccgc tcgcactgtg cacgcacgaa ccggttcggc
    13141 tcggctcccc acacgagctc gttggctgcg tacttctcgt cccactgttc cgcatccacg
    13201 ccgaccaact tagtgtcccg cgtcggaagt cttttcccgt tgcgccggtc caggcggcgc
    13261 ctcgcggcgt tgtcgtcggt cgacgatgct gcgcatcgcc tccc
//
LOCUS       DATBFZ010000374         2753 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_1897197, whole genome shotgun sequence.
ACCESSION   DATBFZ010000374 DATBFZ010000000
VERSION     DATBFZ010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1897197"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="VH419_11310"
     CDS             <1..396
                     /locus_tag="VH419_11310"
                     /inference="COORDINATES: protein motif:HMM:NF018663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY7044246.1"
                     /translation="HVDKVWRSPSETPSGPAGMVLTVDFTVAGQQFQGLNGGPNFTFN
                     EAVSFVIECDDQAEVDRLWEALTAGGGEPGPCGWLKDRFGLSWQIVPRRLDELLNDPD
                     SQRARRAMDAMLTMGKIDVAELERAADGS"
     gene            complement(404..763)
                     /locus_tag="VH419_11315"
     CDS             complement(404..763)
                     /locus_tag="VH419_11315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044247.1"
                     /translation="MTEEHDIAYAALAHGTPVRSSDGVVVGTVERVLDIPDLDLFDGI
                     VIETSQGIRFVDRDHIARITNMAVYGAFTSDEAATLPVPDSAPTYQASAGRFGGSSGW
                     IRRLFGRPSWKRDRDDQ"
     gene            977..2509
                     /locus_tag="VH419_11320"
     CDS             977..2509
                     /locus_tag="VH419_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY7044248.1"
                     /translation="MSTTATSPIRSTIPARLDRLRWTPFHTRMVAGLGAAWILDGLQI
                     TISSSVTGVLSSPKTLGMSSTEIGAIASIYLVGEMVGALVFGRMSDRLGRKRLLITTL
                     LLYLLGTGLAAFVTGHHNGWLVFFYLTRFVAGMGIGGQYAAINSAIDEMMPSKYRGRV
                     DIWINGTYWAGAIIGSFASLIFLHAFAVNVGWRLAFLMGPVLALVVIIVGRTLPESPR
                     WLMTHGRVEEAEKELARIEDVARRSGQELGPIDDSQAITLVPEKQYGYLVFLRLVFRD
                     YPKRAILGATLMITQSFLYNAIFFTYALVLVNFYDVSAKTVPIYGLAFSVGNLLGPLI
                     LGPLFDTVGRKPMIGGTYLLSGALLMVSAWLFGENDLTARSQTFVWVVIFFFASAGAS
                     AAYLTVSETWPIEIRAEAIAVFFAIAQIFGALGPLFYGALIGDGTSRTGMVWGYVIGG
                     LIMVIGGVVELLIGIKAEGQSLENVTKPLTSVSDGEQSDDEQHVPAPRTSDVSERDGI
                     RR"
     gene            2493..>2753
                     /locus_tag="VH419_11325"
     CDS             2493..>2753
                     /locus_tag="VH419_11325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044249.1"
                     /translation="MASAVEDRVVNLAGLAQGVTLVTFPAASSIFTGPDEYDLSSTQY
                     GAMFLPQVATAIASSLLGAGLAQRTSSKRVLLAGLTTNIVAMV"
BASE COUNT          430 a          926 c          908 g          489 t
ORIGIN      
        1 cacgtcgaca aggtctggcg gtcgccgagc gagacgccct ccggaccggc cggcatggtc
       61 ctgacggttg acttcaccgt cgccggccag cagttccaag ggctcaacgg tggcccgaac
      121 ttcacgttca acgaggcggt gtcgttcgtg atcgaatgcg acgaccaggc cgaggtcgac
      181 cggctctggg aggcgctgac ggccggcggc ggtgagcctg gaccctgtgg ctggctcaag
      241 gaccggttcg gactttcctg gcagatcgtg ccgcggcgac tggacgagct gctcaacgat
      301 cccgattccc agcgtgctcg ccgggcgatg gacgcgatgc tcacgatggg caagatcgac
      361 gtcgcggagc tggagcgagc tgccgacgga tcctgagccg ggctcactgg tcgtcgcggt
      421 cacgcttcca cgaggggcgt ccgaacaacc gccggatcca acccgagctg ccgccgaacc
      481 ggccggcgct cgcctggtag gtgggagcac tgtccgggac gggcagtgtc gcggcctcgt
      541 cggaggtgaa ggcgccgtac accgccatgt tggtgatccg cgcgatgtgg tcgcggtcga
      601 cgaaccgaat gccttgcgac gtctcgatca cgatcccgtc gaagaggtcg agatccggga
      661 tgtcgagcac ccgctccacc gtcccgacca ccacgccgtc gctggaccgc accggggtgc
      721 cgtgtgccag ggcggcgtac gcgatgtcgt gttcctcggt catgcctcga aactacccgg
      781 gaacgaccgg tgctttctcg gcttcgtcac caaccgggct ctcgggcggg cggggaccat
      841 gtcctgagtg cgtcgtgccg cgaactcgat gacgtttgtc ggatgccgcg acggaaccac
      901 tccgcacgga tgaggcaacg cgtcgatcga cgggtggatg ctccctccag gccacacagc
      961 agaaagggcc gggaccatgt ccactaccgc aaccagcccc atccgttcca cgatccccgc
     1021 tcggctcgac cggcttcgct ggacgccgtt ccacacccgc atggtggccg gtctcggcgc
     1081 cgcctggatt ctcgacgggc tgcagatcac catctcgagc tcggtcaccg gcgtgctctc
     1141 cagccccaag acgctgggga tgtcctcgac ggagatcgga gccatcgcgt cgatctacct
     1201 ggtcggcgag atggtcgggg ctcttgtctt cggacggatg tccgaccggc tcggccgcaa
     1261 acggctgctc atcacgacct tgctcctcta tctgctgggc accgggctgg cagccttcgt
     1321 gaccggtcac cacaacggct ggctggtctt cttctacctg acccgtttcg tcgccggcat
     1381 gggcatcggt ggtcagtacg ccgccatcaa ctcagcaatc gacgagatga tgccgtcgaa
     1441 gtaccgcggc cgtgtcgaca tctggatcaa cggcacctac tgggccggcg cgatcatcgg
     1501 gtcgttcgcg tcgctgatct tcctgcacgc cttcgccgtc aacgtgggct ggcggctcgc
     1561 gttcctgatg ggtcccgtgc tcgcactcgt ggtcatcatc gtcggccgga cgcttccgga
     1621 gagtccccgc tggctgatga cgcacggccg ggtcgaggag gccgagaagg agctcgcccg
     1681 catcgaggac gtcgcccgcc ggtccggaca ggagctcggt ccgatcgacg acagccaggc
     1741 gatcacgttg gttccggaga agcagtacgg ctacctggtc ttcctgcgcc tggtgttccg
     1801 cgactacccc aagcgcgcca tcctcggggc cacgctgatg atcacccagt cgttcctcta
     1861 caacgccatc ttcttcacct acgcgctggt cctggtgaac ttctacgacg tctccgccaa
     1921 gacagtcccg atctacggac tcgcgttctc cgtcggcaac ctgctcgggc ccctgatcct
     1981 ggggccgctg ttcgacacgg tcgggcgcaa gccgatgatc ggcggcacct acctgctctc
     2041 gggcgcgctg ctgatggtca gcgcctggct gttcggtgag aacgacctga cggcccggag
     2101 ccagacgttc gtctgggtgg tgatcttctt cttcgcctcg gccggagcca gcgcggccta
     2161 cctcacggtg agcgagacct ggccgatcga gatccgggcc gaggcgatcg cggtgttctt
     2221 cgcgatcgcg cagatcttcg gagccctcgg cccactgttc tacggtgcgc tgatcgggga
     2281 cggcacgagc cggaccggca tggtctgggg ctacgtgatc ggcggcctga tcatggtcat
     2341 cgggggcgtg gtcgagctgc tcatcggaat caaggccgaa ggccagtccc tcgagaacgt
     2401 caccaaaccg ttgacgtcag tgtcggacgg cgaacagtca gacgacgaac agcacgtgcc
     2461 tgccccgcgc acgtccgacg tgagcgagcg tgatggcatc cgccgttgag gatcgtgtcg
     2521 tcaacctcgc ggggctggca cagggcgtca ccctggtcac cttcccggcg gcgagctcga
     2581 tcttcacggg cccggacgag tacgacctga gcagcacgca gtacggggcg atgttcctgc
     2641 cgcaggtcgc gaccgcgatc gcgtcctcgt tgctcggagc gggtctcgcg caacggacga
     2701 gctcgaaacg ggtgctgctc gcgggcctga caacgaacat cgtcgcgatg gtc
//
LOCUS       DATBFZ010000375        11526 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_3210385, whole genome shotgun sequence.
ACCESSION   DATBFZ010000375 DATBFZ010000000
VERSION     DATBFZ010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 11526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11526
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3210385"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..853
                     /locus_tag="VH419_11330"
     CDS             <1..853
                     /locus_tag="VH419_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020391392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TRCF domain-containing protein"
                     /protein_id="HEY7044250.1"
                     /translation="ESGLDVPNANTLIVERSDLLGLSQLHQLRGRVGRGGERAYAYFF
                     YPPEKPLTETAHDRLATIAQHSDLGAGMAVAMKDLEIRGAGNLLGGEQSGHIADVGFD
                     LYVRLVGEAVSEYRGDGPVEEPEVRIELPVDAHLPHEYVPSERLRLEMYKRLAEVRTS
                     LQVDEIRAELIDRYGSLPDPVENLLAVAMLRVKARQAGMSDINVQGRYVRFSPVDLPD
                     SARVRIDRIYPKTVVKSALHSMLVPRPTAGANGGQPLRDVAVLHWASRVIEDAVPAAR
                     PAPAAQS"
     gene            948..1625
                     /locus_tag="VH419_11335"
     CDS             948..1625
                     /locus_tag="VH419_11335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044251.1"
                     /translation="MAATAAATVLLLGACSSNLQPGNAAVVNGAAIPQSEVDDLVSAG
                     CAYEAAMGKTDRETYPLLSLASLRTNFLNVLIRNHLARQAADEIGGLHVSQNQVSSLA
                     AGNTIPPTLDDQERSRVEDFFHTSAETDLLVALIGAHLKDDTVTDASHVQQADLDAGQ
                     KYLDAYTKKADVKVNPRFGTWTGTTVKGGSGSLSQLASTPPTPAAIPGQPAPNPAETL
                     PPDQVCG"
     gene            2018..2629
                     /locus_tag="VH419_11340"
     CDS             2018..2629
                     /locus_tag="VH419_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002526952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG family protein"
                     /protein_id="HEY7044252.1"
                     /translation="MVMARLRRECPWDQSQTHESLVRYLLEEAYETIEAIETGDRDHL
                     REELGDLLLQVMFHSRIASEHTDEPFDVDDVAGQIVEKLVRRHPHVFGDVTVESAQDV
                     NENWETIKAAEKSRSSAMEGIPPGLPALAWADKVVGRTMSAGLAPSVPTPDEAAYTAE
                     TLGEVLFALVAAAHAAGIDAEQALRQRVRAEIDAVRNVETRPT"
     gene            2757..4043
                     /gene="eno"
                     /locus_tag="VH419_11345"
     CDS             2757..4043
                     /gene="eno"
                     /locus_tag="VH419_11345"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEY7044253.1"
                     /translation="MALIEAVGAREILDSRGNPTVEVEVALDDATIARAAVPSGASTG
                     QFEAVELRDGGDWYAGKGVGKAVSAVNDEINEAITGMSADEQRLVDQALLDLDGTPNK
                     ARLGANAILGVSLAVARAAADSAGLPLFRYIGGPNAHLLPVPMMNILNGGAHADSNVD
                     VQEFMIAPVGAPTFAEAMQHGAAVYHSLKAVLKERGLATGLGDEGGFAPNLDSNREAL
                     DLIASAVEKAGLTLGRDVVLALDVAATEFYDSDAGHYTFEGAAKSSDDMAAYYSKLVD
                     SYPIVSIEDPMSEEDWDGWTSMTAAVGDRIQIVGDDLFVTNVDRLQRGIDQNAANALL
                     VKVNQIGSLTETLDAVDLAHRSGMRCMMSHRSGETEDTTIADLAVATNCGQIKSGAPA
                     RSERVAKYNQLLRIEEELDDAARYAGRGAFPRSTGS"
     gene            4198..4725
                     /locus_tag="VH419_11350"
     CDS             4198..4725
                     /locus_tag="VH419_11350"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEY7044254.1"
                     /translation="MAAPETTARASSSRAKLTGRAAILLLVVAVLAVSYASSMRAWLK
                     QRSDINQLNADIARQSGDIAALRQAKKRLHDPAYIREQAHERFGWVMPGETSYRVIGA
                     DGDVLPDGTSALSDPADVFSRKNPEWWQQVYGSVVEAGRPETEEQTPSPADTLRRPPA
                     KSGSTGPRGGQQTDR"
     gene            4782..5201
                     /locus_tag="VH419_11355"
     CDS             4782..5201
                     /locus_tag="VH419_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012923254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF501 domain-containing protein"
                     /protein_id="HEY7044255.1"
                     /translation="MVDVAHRCPCGNPDVVATAPRLEDGTPFPTFYYLTCPRAASEIG
                     TLEASGLMREMTDRLEADADLAARYRAAHDAYLRDRDEHGAVPEIAGVSAGGMPDRVK
                     CLHVLVAHSLAAGPGINPLGDEALALLPDWWASGPCV"
     gene            5307..6260
                     /locus_tag="VH419_11360"
     CDS             5307..6260
                     /locus_tag="VH419_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017936179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HEY7044256.1"
                     /translation="MRRRVAAVDCGTNSLRLLVADLEPASGTTNWVDRRTTIVRLGQG
                     VDATGSFRSEALERTFAAIDEYADVIAELGAVDVRCVATSAARDVANRDVFLDGVRDR
                     LGVEPEIISGDEEAQLSYDGATRELARHGAVDEPVLVVDIGGGSTEFVMRDSPDGTVV
                     GQSLDIGSVRLTERRLEDDPPTSEQVNAAVRDVEDALATLTVPVATARTVVGVAGTVT
                     TMGAMVLDLDRYDRDRVNGSRLASADVLAAVDEIVAMTVAERRALAFMHPDRADVIGA
                     GALVLGGIVGHLGLPEFRASSHDLLDGIVWSLMPAHQSDAG"
     gene            6257..7153
                     /locus_tag="VH419_11365"
     CDS             6257..7153
                     /locus_tag="VH419_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015070391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEY7044257.1"
                     /translation="MTGLPHPLTGDLFDSPVPAGAGWPDDPARPDTPVALAAADVRRL
                     AGSASDLAALEAAVSVCRACSRLVSWREDVAATKRRGFAAEPYWGRPIAGWGADQPRV
                     LVVGLAPAAHGGNRTGRIFTGDRSGDWLFASLYRVGLATQPTSVYAGDGQALIATRMM
                     ATVRCAPPANKPTPVERDTCAPWLDAELRQVLPTTRVVVCLGSYGWEAALRALARAGL
                     TIPRPRPRFGHGSRVDLVSQVSPAGGEVKGGLVVLGCYHPSQQNTFTGKLTESMLDAV
                     LGEAARLAAIVPPPATPATPAP"
     gene            7318..8586
                     /locus_tag="VH419_11370"
     CDS             7318..8586
                     /locus_tag="VH419_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007078868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEY7044258.1"
                     /translation="MSQTSDSTASTGLARRLGVTDAVVIGLSAMIGAGVFAVFAPAAQ
                     AAGSLLLVGLGIAAFVAYCNATSSAQLAAQYPTSGGTYVYGRERLGEWWGFVAGWGFV
                     VGKTASCAAMALTFAAYAVQGPMWVQRVVALGAVAALTTVNYHGISRTSQLARLLLSV
                     TLVGLVVGIAAVAVQALDGPSRIGTGEAWSTGGWYGVLQSAGLIFFAFAGYARIATLG
                     EEVRDPEQTIPRAIPTALAITVILYAVVATVLLLALGPAALAASPAPVATAAEQVADW
                     TGWLVRAAAVAASLGALLALVAGVGRTTLAMAREGDLPRSLDRVHPRHRVPHRAEVAV
                     GCIVGVLVLTTDLRGVIGFSSFGVLVYYAVANASAFTQDAGHRRWPRVLQLLGVAGCL
                     LLVVTLPWEAVVAGAAVFALGCGGRAVRRR"
     gene            8702..10003
                     /locus_tag="VH419_11375"
     CDS             8702..10003
                     /locus_tag="VH419_11375"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020576139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY7044259.1"
                     /translation="MSKRTPHIVIAGGGYVGMYTALRLQRKLRRGEARVTVIDPRSTM
                     TYQPFLPEAAAGNLEPRHVVVSLRRTLRHCNVLTARVASVDHAHQTLRVEPPESAAYD
                     LHYDELVIAMGSVSRTLPIPGLAEHGMGFKQIEEAIALRNHVLDKLDLATTVVDPRER
                     TAALTFVFVGGGYAGVEALAEIEDMVRYATRFYDTIQESDVRFVLVEALNRILPEVGE
                     DMGRYTVDRLRKRGIEVRLNTRLESAVDRHIVLSDGEEFDADTLVWTAGVKATPVLLN
                     TDLPLDDKGRLKARPDLTVEGADHVWAAGDNAAVPDLTQPGALTAPNAQHAVRQARVL
                     GDNIVASLRGEPLKEYHHKYVGSVASLGLHKGVAQVYGVKLRGFPAWFMHRTYHLSRM
                     PTLSKKARVVLDWTLALFFKRDVVALGALQHPRDDFDRANR"
     gene            10038..10111
                     /locus_tag="VH419_11380"
     tRNA            10038..10111
                     /locus_tag="VH419_11380"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10072..10074,aa:Leu,seq:taa)
     gene            10219..11067
                     /locus_tag="VH419_11385"
     CDS             10219..11067
                     /locus_tag="VH419_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007078427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEY7044260.1"
                     /translation="MQSRNPILTNSDTFNGRAAATYGHQSYPASGQAYQGYGQVPPAP
                     TPAGSDPSTWQVPTDRALEPRMTIDSVVTRTALTLFLVVVTAGITWVYLPESLTSTAW
                     IGGALVGAGLGIFLSFQRTVSPPLVAVYAIAQGFFLGAASEAFNQIWPGVVAQAVGGT
                     MAAFAATLAAYKFFNIQVSDRTRKFVVIAGLGFFALVMTDFLLSLFGFAIGFNGMGGL
                     GLVMSIIGFALGVFFLILDFDMVEQGVAAGAPESEAWRAAFGLTATLIFIYIELLRIL
                     AILRGD"
     gene            complement(11359..>11526)
                     /locus_tag="VH419_11390"
     CDS             complement(11359..>11526)
                     /locus_tag="VH419_11390"
                     /inference="COORDINATES: protein motif:HMM:NF025482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4287 domain-containing protein"
                     /protein_id="HEY7044261.1"
                     /translation="GRPLQEWFGHLENGPGCLRFDGRVQWLRDEYNLSHGHATAIVHE
                     HDLIKAHRSFE"
BASE COUNT         1763 a         3905 c         4085 g         1773 t
ORIGIN      
        1 cgagtcgggc ctcgacgtgc cgaacgcgaa cacgctcatc gtcgagcggt ccgacctgct
       61 gggcctgtcc cagctgcacc agctccgcgg tcgggtcggc cgtggcggcg aacgtgccta
      121 cgcctacttc ttctatccgc cggagaagcc gttgaccgag accgcgcacg accggttggc
      181 gaccattgcc cagcactccg atctcggggc cgggatggcg gtggcgatga aggacctcga
      241 gatccgcggc gccgggaacc tgctgggcgg agagcagtcc ggccacatcg ccgacgtcgg
      301 cttcgatctg tacgtgcgac tggtcggtga ggcggtgtcg gagtaccgcg gagacgggcc
      361 ggtcgaggag ccggaggtca ggatcgagct gccggtggac gcccacctcc cgcacgagta
      421 cgtgcccagt gagcgcctgc gtctcgagat gtacaagcgg ctggcggagg tgcggacctc
      481 gttgcaggtc gacgagatcc gggccgagct gatcgaccgg tacggctcac tgcccgaccc
      541 ggtcgagaac ctgctcgccg tcgcgatgct gcgggtcaag gcacggcagg ccggcatgag
      601 cgacatcaac gtgcaagggc ggtacgtcag gttctccccc gtcgacctgc cggactccgc
      661 ccgggtgcgc atcgaccgca tctacccgaa gacggtcgtg aagagtgcgt tgcatagcat
      721 gctggtgccg cggccaacgg caggtgcgaa cggcggacag ccgctgcgcg atgtcgccgt
      781 actacattgg gccagtcgtg tgatcgagga cgccgtgcct gcggctcggc cggcgcccgc
      841 ggcgcagtcg tgacgacacg tccagctgca acgaaggagc gagcatcccc gtgacagccc
      901 ccaagccgaa ccggccgctg cgcggcagac gagcttcgag acttgtcatg gccgcgactg
      961 cggcggcgac cgtgctgctc ctgggcgcct gttcgtccaa cctgcagccg ggcaacgcgg
     1021 cggtcgtcaa cggcgccgcc atcccgcaga gcgaggtcga cgatctcgtc tcggcgggct
     1081 gtgcgtacga agccgcgatg ggaaagaccg accgagagac ctatccgctg ctctcgctgg
     1141 cgtcgcttcg gacgaacttc ctcaacgtgc tgatccgcaa ccacctggcg cgccaggccg
     1201 ccgacgagat cggcgggctg catgtcagcc agaaccaggt ctcgagcctg gcggccggca
     1261 acacgatccc gcccacgttg gacgaccagg agcggagccg ggtcgaggac ttcttccaca
     1321 ccagcgcaga gacggatctc ctcgtcgcgc tgatcggagc ccacctgaag gacgacacgg
     1381 tgacggatgc gtcacacgtg caacaggcgg acttggatgc tgggcagaag tacctcgacg
     1441 cgtacacgaa gaaggctgac gtcaaggtca acccgaggtt cggcacctgg accggtacca
     1501 ccgtcaaggg tggcagcggg tcgctgtcgc agctcgcatc gacgccaccc acccccgctg
     1561 cgatccccgg ccagccagca cccaatccag cagagacgct gccgcccgac caggtgtgcg
     1621 gctaggttcg gttagccctg cgatgcctga ccggcacgcg ctcacggtcc tccagacgac
     1681 gccccggctg ccggcaggac agcttaccgg cccggcctgg caagcgatac tgtccgccga
     1741 ccaggtgctc acggcggacc cttcgagtcc gctggcgctt gccgtggtcg cagccggcgt
     1801 cactgtcggg caggctcacc ctgatgcagc ggcgctgctg gcgcgggcga gcgtatccca
     1861 cgtcgtctgg ctggcggcgg aggccggaga cgaggagctg acctcggccc tggcggcaga
     1921 cgtcgtacgc cgttcggagt ccggcgaaca gggcccggag gtcgaggtcg tgctcggctc
     1981 gttcgatcct cctggggcgc ggctgctgga tctggtcatg gtcatggccc ggctgcgtcg
     2041 ggagtgtccc tgggaccaga gccagaccca cgagtcgctg gtgcgctacc tgctcgagga
     2101 ggcctacgaa accatcgagg cgatcgagac aggtgaccgc gaccacctgc gcgaagagct
     2161 cggcgacctg ctgctccagg tcatgttcca cagccggatc gccagcgaac acaccgacga
     2221 gccgttcgac gtcgacgacg tggcaggaca gatcgtggag aagctcgtcc ggcggcatcc
     2281 gcacgtgttc ggcgatgtca cggtcgagtc cgcgcaggac gtgaacgaga actgggagac
     2341 gatcaaggct gccgagaagt cgcgctcgtc ggcgatggag gggattcccc cgggactgcc
     2401 cgcactggcg tgggccgaca aggtggtggg ccggacgatg tctgccgggc tcgcgccgag
     2461 cgttcccacg ccggacgagg cggcgtacac cgcggaaacc ctcggggagg tcctgttcgc
     2521 gctggtcgcc gcggcgcatg ctgccggcat cgacgcggag caggcgcttc gacagcgggt
     2581 ccgtgccgag atcgacgccg tacgaaacgt ggaaacgcgc ccgacgtagt cgacaagaga
     2641 tagctgggtc gtgatgtgcc cgggccggag gacgccgggc cgcgtgccgt gcgcgatcgg
     2701 cgagcgtgcc ggcggatagg ctgagcgaat ccggctcgac gaggtaggag gagcccatgg
     2761 cgctgatcga ggccgttggc gctcgggaga tcctcgactc ccgcggcaac cccaccgtcg
     2821 aggtcgaggt cgcgctcgac gacgccacga tcgcgcgggc ggccgttccg tcgggggcgt
     2881 cgacaggcca gttcgaggcc gtcgagctgc gcgacggagg cgactggtac gccggcaagg
     2941 gtgtcgggaa agcggtctcc gcggtcaacg acgagatcaa tgaggcgatc accgggatgt
     3001 ccgccgatga gcaacgactc gtcgaccagg cgctcctcga cctcgacggc acaccgaaca
     3061 aggcgaggct cggcgcgaac gcgatcctcg gtgtctcgct cgcggtggcc cgtgcggccg
     3121 ccgactcggc cggactgccg ctgttccgct acatcggcgg gccgaacgcc cacctcctgc
     3181 cggtcccgat gatgaacatc ctcaacggcg gggcgcatgc tgacagcaac gtcgacgtcc
     3241 aggagttcat gatcgcaccc gtcggggcgc cgacgttcgc cgaggccatg cagcacgggg
     3301 cggcggtcta ccactcactg aaggcggtgc tgaaggagcg tggactcgca accggactcg
     3361 gtgacgaggg cggcttcgct ccgaacctcg actcgaaccg tgaggcgctc gacctcatcg
     3421 cgtcggcggt cgagaaggct ggtctgacgc tcggccgaga cgtcgtactc gcgctcgacg
     3481 tcgctgcgac cgagttctac gacagcgacg cgggtcacta cacgttcgag ggagcggcga
     3541 agtcatccga cgacatggcg gcgtactaca gcaagctggt cgactcctac ccgatcgtgt
     3601 cgatcgagga tccgatgtcg gaggaggact gggacggctg gacgtcgatg acggcggccg
     3661 tcggtgacag gattcagatc gtcggcgacg acctgttcgt gacgaacgtc gaccggctcc
     3721 agcgcggtat cgaccagaac gccgcgaacg ccctgctcgt gaaggtcaac cagatcggca
     3781 gcctgaccga gaccctcgac gcggtcgacc tcgcgcaccg cagcgggatg cgctgcatga
     3841 tgagccaccg ctccggcgag accgaggaca ccacgatcgc cgacctcgcc gttgcgacga
     3901 actgtggtca gatcaagtcc ggggcgccgg cgcggtcgga gcgcgtcgcc aagtacaacc
     3961 agctgctccg gatcgaggaa gagctcgacg atgccgcccg ctatgcgggc cgtggcgcct
     4021 tcccgcgctc caccggttcg tgagccgcgg ctgactgggc tggctgggct gtcaatggtg
     4081 tcccgttcgc gttcgagctc cgctcctggt ccgggccggg ggcggcgtac gccgaccggc
     4141 tcgacccggc ggtctggcag gtcgagcagt acgcggaagg ctcgcaccac gacgcgggtg
     4201 gccgctcccg agacaaccgc gcgagcgtcc tcgtcgaggg cgaagctcac gggacgggcg
     4261 gccatcctgc ttctcgtggt cgccgtcttg gccgtgtcgt acgcgtcgag catgcgcgcc
     4321 tggttgaagc agcgcagcga catcaaccag ctcaacgccg acatcgcccg ccagagtggg
     4381 gacatcgccg cgctgcggca ggccaagaag cggttgcacg acccggcgta catcagggag
     4441 caggcgcacg agcgcttcgg ctgggtcatg ccgggggaga cgagctatcg ggtgatcggt
     4501 gccgacggcg acgtgctccc cgacgggacc agtgcgctct cagacccggc ggacgtgttc
     4561 tcgcggaaga atcctgagtg gtggcagcag gtctacggca gcgtcgtcga ggcaggtcgg
     4621 ccggagaccg aagagcagac gccgagcccg gcggatacct tgcgacgccc acccgccaag
     4681 tccggcagca cgggtccccg aggcgggcag cagaccgacc ggtgaccgat ccgcgagacg
     4741 tggcggcggt gcgggcgcag ctcggccgcc cgccacgcga catggtcgac gtcgcgcatc
     4801 gttgcccgtg cgggaacccg gatgtggtgg ccaccgcgcc acggctggag gacggcacgc
     4861 cgttcccgac cttctactac ctgacctgcc cgcgcgccgc gtcggagatc ggcacgctcg
     4921 aggcgagtgg actgatgcgg gagatgaccg accggctgga agcggacgct gacctcgccg
     4981 cacgctaccg ggcggcgcac gatgcgtatc ttcgcgaccg ggacgagcac ggcgcggttc
     5041 cggagatcgc cggggtctcc gccggcggga tgccggaccg ggtcaagtgt ctgcacgtgt
     5101 tggtcgcgca ctcgctggct gccggccctg ggatcaaccc gctcggcgac gaagccctgg
     5161 ccctcctccc cgactggtgg gcctcaggcc cctgcgtctg accaggccgg cgtgatcgag
     5221 gtggcaacca gtgtccgaca cgccgtccaa acgcaacgaa acctgccacc tcggcggggc
     5281 ctgaccgcgg ctcgtagggt gagcgggtga ggcggcgggt ggcggcggtc gactgtggaa
     5341 cgaactcgct gcgcctgctc gtcgcagatc tcgagccggc ctccggcacc accaactggg
     5401 tcgaccggcg tacgacgatc gtccggctcg gacaaggcgt cgacgcgacc ggttcgttcc
     5461 ggtcggaggc gctcgaacgg acgttcgcgg cgatcgacga gtacgccgac gtgatcgccg
     5521 agctcggcgc cgtcgacgtg cgctgcgtcg cgacgtcggc cgcgcgtgac gtggccaacc
     5581 gggatgtgtt cctcgacgga gtacgcgacc ggctcggggt cgagccggag atcatcagcg
     5641 gcgacgagga ggcccagctc tcgtacgacg gcgcgacccg cgaactcgcc cgccacggtg
     5701 ctgtcgacga gccggttctc gtggtggaca tcggtggcgg ctcgacggag ttcgtcatgc
     5761 gggactcgcc cgacggcacg gtcgtcggac agtcactgga catcggatcg gtccggctca
     5821 cagagcgccg actcgaggac gacccgccca cgtccgaaca ggtgaacgcg gcagtgcggg
     5881 acgtcgagga tgcgctggcg acgctgacgg tcccggttgc aacggccagg acagtcgtcg
     5941 gagtcgccgg caccgtgacg acgatgggcg cgatggtgct tgatctggac aggtacgacc
     6001 gggaccgcgt caacggttcc cggctggcga gtgccgacgt gctggcggcg gtcgacgaga
     6061 tcgtcgccat gacggtggcc gagaggcgtg cgcttgcgtt catgcatccc gaccgtgccg
     6121 acgtgatcgg cgccggtgcg ctggtcctcg gcggcatcgt cggtcacctc ggcctccccg
     6181 agttccgggc ctcctcacac gacctcctcg acgggatcgt ctggagtctc atgccggctc
     6241 accagtctga tgccggatga ccgggcttcc tcatccgctc accggtgatc tcttcgacag
     6301 tccggtcccg gccggggccg gctggccgga cgacccggcc cggccggaca cgccggtggc
     6361 gctggcggcc gccgacgtac gccggttggc tggctcggcg tcggacctcg cggcgctgga
     6421 ggcggcggtg tcggtctgcc gtgcgtgctc gcgcctggtc agctggcgcg aggacgtcgc
     6481 ggcgacgaaa cggcgcggct tcgccgccga gccgtactgg gggcggccga tcgcgggctg
     6541 gggcgcggac caaccgcgtg tcctcgttgt cgggctcgcg cccgcggctc acggtggcaa
     6601 ccggaccggg cggatcttca ccggcgaccg gtcgggggac tggctcttcg cctcgctgta
     6661 ccgggtcggg ctggccacgc agccgacgag cgtgtacgcc ggcgacggtc aggcgctgat
     6721 cgccacccgg atgatggcga ccgtccggtg cgcgccaccg gccaacaagc ccacgccggt
     6781 cgaacgggac acgtgtgccc cgtggctgga cgcggagctg cggcaggtgc tgccgacgac
     6841 ccgagtggtg gtgtgcctcg ggtcgtacgg ctgggaggcg gcgctacggg ccctggctcg
     6901 cgccggcctg acgatccccc ggccgcgacc ccggttcgga cacgggtcgc gtgtcgatct
     6961 ggtctctcag gtgtcgccgg cgggtggcga ggtgaagggc ggactcgtcg tgctcggctg
     7021 ttaccacccg agtcagcaga acacgttcac cgggaagctc accgagtcca tgctcgatgc
     7081 ggtcctcggt gaagccgccc gcctcgctgc catcgtcccc ccgcccgcca cccccgccac
     7141 ccccgctccc tgaccctccc cgccgctcgt ggtgctccgc gaattgcggg aaagtggcgg
     7201 tcgacacgcg ggtccggccc tgacttttcc cgcaattcgc ggaagcccgg cagctgcgga
     7261 aggtacgcaa gaaggatcaa gtccggcgac gacggctgcg tcagactgag aactgccatg
     7321 tcccagacga gtgactcgac cgcatcgacc gggctggccc gacgattggg ggtcaccgac
     7381 gccgtcgtga tcggcctgtc cgcgatgatc ggcgccggcg tcttcgcggt cttcgcgccg
     7441 gctgcacagg cggccggcag cctgctcctc gtcggcctcg gaatcgcggc gttcgtcgcg
     7501 tactgcaacg ccacctcctc cgcgcagctc gccgcccagt acccgacgtc cggcggcacc
     7561 tacgtctacg gcagggagcg gctcggcgag tggtgggggt tcgtcgcggg gtgggggttc
     7621 gtggtcggga agacggcgtc ctgcgcagcg atggctttga cgttcgcggc gtacgccgtc
     7681 cagggcccga tgtgggtgca gcgggtggtg gcgctcggag cggtcgcggc cctgaccacc
     7741 gtgaactatc acggcatctc ccgcacctcg cagctggcgc gactgctgct gtccgtcacc
     7801 ctcgtcgggc tcgtcgtcgg gatcgccgcg gttgcggtgc aggcgctcga cggaccctcg
     7861 cggatcggga ctggtgaagc ctggtcgacc ggtggctggt acggcgtcct gcagtccgcc
     7921 gggctgatct tcttcgcctt cgccggatac gcgcgcatcg ccacactcgg tgaggaggtg
     7981 cgcgatcccg agcagacgat cccgcgggcg atcccgaccg cgctggccat caccgtgatc
     8041 ctctacgccg tggtcgcgac cgtgctcctg ttggcgctcg gtccggctgc gctcgcggcc
     8101 agccccgctc cggtcgcgac ggccgctgag caggtggccg actggacggg ctggctggtg
     8161 cgggcggccg cggtcgccgc cagtctcgga gccctgctcg ccctcgtcgc cggtgtcggc
     8221 aggacgacgt tggcgatggc ccgcgaaggc gacctgccgc gatccctcga tcgggtgcat
     8281 cctcgtcacc gcgtcccgca tcgcgccgag gtggcggtcg gctgcatcgt cggcgtgctg
     8341 gtcctgacga cggatctgcg cggcgtgatc ggtttctcgt cgttcggcgt actcgtgtac
     8401 tacgccgtcg ccaacgccag cgcgttcacc caggacgccg gccaccggcg ctggccgcgg
     8461 gtactccagc tgctgggcgt ggccggctgc ctgctgctcg tcgtcacgct gccgtgggag
     8521 gcggtcgtcg ccggcgcggc ggtgttcgcg ctcggctgtg gcggccgcgc cgtacgccgt
     8581 cggtgacgca ggaccgtctg cgtgaagagg actcgcggaa actcacttgt gaaagttttc
     8641 acgaacacta ggctgggagt agaggctatg gacaggctcg agcagggaaa ggggaacctg
     8701 gatgagcaag aggacgccgc acatcgtgat cgccggaggc ggctacgtcg ggatgtacac
     8761 agccctgcgc ctccagcgca aactgcgtcg tggtgaggca cgggtgaccg tgatcgatcc
     8821 tcgctcgacg atgacctatc agccgttcct ccccgaggcg gccgccggca acctcgaacc
     8881 tcgccacgtc gtcgtctcgc tgcgccggac cctgcgtcac tgcaatgtcc tgacggcgcg
     8941 ggtggcgtcg gtcgaccacg cgcaccagac gttgcgcgtc gagccgccgg agagcgcggc
     9001 gtacgacctg cactacgacg agctcgtcat cgcgatgggc tcggtctcgc gcacgcttcc
     9061 catcccgggt ctggcggagc acggcatggg gttcaagcag atcgaggaag cgatcgcgct
     9121 gcgcaaccac gtgctcgaca agctcgacct cgcgacgacc gtcgtcgacc cgcgagagcg
     9181 gacggcggcg ctgacgttcg tgttcgtcgg gggcggatac gccggtgtgg aagcgctggc
     9241 cgagatcgag gacatggtcc ggtacgccac ccgtttctac gacaccatcc aggaaagcga
     9301 cgtccggttc gtgctcgtgg aggcgctgaa ccgcatcctg cccgaggtgg gcgaggacat
     9361 gggccgctac acggtcgacc ggctccgcaa gcgcggcatc gaagtgcggc tcaacacgcg
     9421 actggagtcg gcggtcgacc gtcacatcgt gctgtcggac ggcgaggagt tcgacgccga
     9481 cacgctcgtc tggacggccg gcgtcaaggc cactcccgtc ctgttgaaca ccgaccttcc
     9541 actggacgac aagggccggc tcaaggcgcg ccccgacctg accgtcgagg gtgccgacca
     9601 cgtgtgggcc gcgggggaca acgccgccgt acccgatctc acccagccag gagcgctgac
     9661 ggcgccgaac gcccagcacg cggtacggca agcgcgggtc ctcggcgaca acatcgtcgc
     9721 gtcgctgcgc ggtgagccgt tgaaggagta ccaccacaag tacgtcggct cggtggccag
     9781 cctcggcctg cacaagggag tggcgcaggt gtacggcgtc aagctgcgcg gtttcccggc
     9841 ctggttcatg caccgcacct accacctcag ccggatgccg acgctgagca agaaggcgcg
     9901 cgtggtgctc gactggacgc tggcgctgtt cttcaagcgc gatgtggtgg cgctgggggc
     9961 cctgcagcac ccccgcgacg acttcgaccg cgcgaaccgc tgagtcgtgg gtcggcctct
    10021 acgatgaagc gcggcacgcc cccgtatccc aattggcaga ggacgcaccc ttaaaaggcg
    10081 ctcagtgtgg gttcgagtcc caccgggggc acgctggtat gcgacccgcg caggcatctc
    10141 gggcgttcgg aggcattcga cgtccccgta tcatggacag gacccagcag agcgctgggc
    10201 cgaggaggag ccgtccccat gcagagtcgt aacccgattc tcaccaacag cgacacgttc
    10261 aacgggcgcg ccgctgcgac ctacggtcac cagagctacc cggcgagcgg acaggcctac
    10321 cagggttacg ggcaggtgcc accggcgccg actccggccg gcagcgaccc gtcgacctgg
    10381 caggttccga ccgaccgcgc gctggagccc cggatgacga tcgacagcgt tgtcacccgc
    10441 accgcgctca ccctgttcct ggtcgtcgtg accgcaggca tcacgtgggt ctatctgccg
    10501 gagtcgctca ccagcacggc gtggatcggt ggcgcgctcg tcggggccgg cctcggaatc
    10561 ttcctcagtt tccagcgaac cgtctcacct ccgctcgtcg cggtgtacgc gatcgctcag
    10621 gggttcttcc tcggtgccgc atcggaggcg ttcaaccaga tctggccggg tgtggtggcg
    10681 caggccgtcg gtggcacgat ggccgccttc gccgcgaccc ttgcggcgta caagttcttc
    10741 aacatccagg tctccgaccg gacccgcaag ttcgtcgtca tcgccgggct cggtttcttc
    10801 gccctggtca tgaccgactt cctgctctcg ctgttcggct tcgcgatcgg cttcaacggg
    10861 atgggcggcc tgggcctcgt gatgagcatc atcggcttcg cgctcggcgt gttcttcctg
    10921 atcctcgact tcgacatggt cgagcagggc gtcgcggccg gtgctccgga gtccgaggcg
    10981 tggcgcgccg cgttcggcct gacggccacg ctgatcttca tctacatcga gctgctgcgc
    11041 attctcgcca tcctgcgcgg cgactagctt cgcctcacga cctgcactga actgaggcgc
    11101 tgaggtagcg gggtcgaggt ggcaggtttc gtcgtcccgt cggcggggtc aggacgaacc
    11161 ttgccacctc gacgaagggc ccggtagggc aagttgagtc agcaacacgc gcgaagtcgc
    11221 cggccgcgtt gacagggtgt gggcgaagtg gcaactttcg ttatccggtc ggcgtgttca
    11281 ggacgaacct tgccacctcg acgaaaggcc cggtagggca agttgagtca gcaacacgcg
    11341 cgaaggcgcc gcctcgggtt actcgaagct gcggtgggct ttgatgaggt cgtgctcgtg
    11401 cacgatcgcg gtcgcgtgcc cgtgcgacag gttgtactcg tcacgtaacc actggacccg
    11461 accgtcgaac cggagacagc cagggccgtt ctcgagatgg ccaaaccact cttgaagagg
    11521 tcgtcc
//
LOCUS       DATBFZ010000376         5210 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_1897892, whole genome shotgun sequence.
ACCESSION   DATBFZ010000376 DATBFZ010000000
VERSION     DATBFZ010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 5210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5210
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1897892"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            158..3340
                     /locus_tag="VH419_11395"
     CDS             158..3340
                     /locus_tag="VH419_11395"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY7044262.1"
                     /translation="MTRTEPAVAVTLLGGVAVRRAGTSVRMTSTRAIAMLAYLVLRAP
                     ARQSRQHLAAVFWPDSSEPQARTNVRRELHQLRRLVPPGCLEVTDTHIGWVDQDSCAV
                     DVRTFLANVARTRAAMRNGAAVGSGARSGLAQLGRQTVEMYAGPLLPGEYDDWAIEAR
                     EGLQRTCIALCDELVMALAESDVRTAIELARRRIQLDPLEEVGYRQLMRLQVATNDRS
                     GALTTYHRCASLLEEQLGVPPTAETAELLTSIIELDSGAEALAPRDPVDVRSHAPDIE
                     LVGRDEDLAAVLAHWHNVRNGHPDLLLLTGDPGVGKSRLLTEVVRQAAADGWAVASSR
                     CFSTSGVALSPVADWLRSPEVAAVRPSLDPVWESEVERLVPTGGGGDRDGTDVLDRSG
                     QALADTWQRHRFYEGLCQALLSVDRPLLLTLDDLHWCDQQTLAWIAFLLRLAADRPVF
                     VLATARADELRHSELRRTVTDFSSAGLLTEHSVEPLTLEATTALAARMFGRPLEPSDA
                     ALLQAASGGYPLYVVEAARASAAVGGDAEVSESGLGVVLRRRLAELSSDAQRTVALAG
                     AIGRDFRLDLLVQASDQDEVTVARAVDELWRRRVLHQVGRGYDFSHDLLRDEAYALVD
                     PAQRWLEHRRVANALTVLNADDLDAVAAQLAEQHERGGRPEEAVPFYFRAAVEAARVY
                     ANAEAVRLFRRCLDIVAELPTSPERDQRELDVRQAMSAPLNALYGYAHHDLRHTFERG
                     VALAESLGRVDRQLSSLVGLWATTFVKGDIDGSHHIATRAIALADRVPALANQAHFSM
                     GGTAVSLGRPELAVEQLDLACDLSEGGISLSVGSRPEIHARAWSAHGYWLMGDDVRAD
                     SACRQAVSDARLLSHPYSLAIALSYASITAQLRGDMTLLDDSLAELTDLCERHSYAYY
                     REWALILSGWRAGGDRGLAQAKLGIAHLSSSGALTRMPYWLSLLADSQRGIGADADAD
                     ASLDAAWTAASQRGDRWWLPEVLRARAKTKPPDTAAKMLRRAEKTAQAQSSPVLAQRC
                     RDDLSALEGVSRQPSRVEPGVRARSIADER"
     gene            3465..4847
                     /locus_tag="VH419_11400"
     CDS             3465..4847
                     /locus_tag="VH419_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020393058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY7044263.1"
                     /translation="MPFDTLAEQLYGSVITPDDPEYHTARAVYNGMIDRHPAAIARCK
                     NSADVVACVNFGREHGLTVSVRGGGHNAGGLGVADDALVIDLSAMRSTTVNPTEHTVR
                     VDGGCTWGDVDNATVPFGMATPSGVVSTTGVGGLTLGGGIGNLTRSFGLAIDNLLSAD
                     VVLADGRLVTANEKSEPDLFWALRGGGGNFGVVTSFTFRCNELGDAGTVVAGPVLYDI
                     ADTADVLRWYRELLPSLPEELNGWFATLTIPGGPPFPEELWGRKACGIVWCYTGPGER
                     ADEVLAPIRDFGSPLLVGLAPMPFSALQSAFDPLLPPGLQWYWRADIAEEITDEAIAI
                     HSKYGEQLPTALSQMHMYPIDGAASRGSADETAFAYRNGGWSTVMVGVDPDPANKDLI
                     SDWAKAYYDELHPTTTGGAYVNFMMDEGEDRVKASYRDNYERLTEVKKTYDPDNFFHV
                     NQNIRPAKQS"
BASE COUNT          814 a         1778 c         1794 g          824 t
ORIGIN      
        1 gtcagcagct cgcctccgcg ctgttctgac aacggcgcat gccccgtacg cccgggtgtg
       61 tcacgctttg acgcagccat cgcggtccca ggtgggaatc cggccggtgc acccgagagt
      121 tcttccccac cgggactacc ttcttggggg aggtcggatg acacgaacgg agcccgcggt
      181 cgcggtcacg ctgctcggcg gcgtggcggt caggcgtgcg ggtacgtcgg tgcggatgac
      241 gtcgacccgg gcgatcgcga tgctggccta tctcgtcctc cgtgcaccgg cgcgtcagtc
      301 ccggcagcat ctggcggcgg tcttctggcc cgactcgtcc gagccgcagg cacgcacgaa
      361 cgtgcgacgc gagctgcacc agctgcgtcg gctcgtcccg ccgggctgtc tcgaggtcac
      421 tgacacgcac atcggctggg tcgaccagga cagctgtgcg gtcgacgtac gaacgttcct
      481 cgcgaacgtc gcgcggactc gcgccgccat gcgcaacggt gccgccgtcg gatcgggcgc
      541 acgatccgga ctggcccagc tcggccggca gacggtcgag atgtacgccg gaccgttgct
      601 ccccggtgag tacgacgact gggcgatcga ggcacgcgag gggctccagc gcacatgcat
      661 cgcgctgtgt gacgagctgg tcatggccct ggcggagtcc gatgtgcgaa ccgcgatcga
      721 gctggcgcgg cggcgcatcc agctggatcc gctcgaggag gtcggctatc ggcaactgat
      781 gcggctccag gtcgcaacca acgacaggtc cggagcactg accacgtacc accggtgcgc
      841 gtccctgctc gaggaacagc tcggtgtccc gccgaccgcc gagacggccg agctgctgac
      901 ctcgatcatc gagctcgact ccggcgcaga ggcgctggcg ccgcgggatc cggtcgacgt
      961 acgcagccat gcgccggaca tcgagctggt cggccgtgac gaagacctgg cggccgtgct
     1021 cgctcactgg cacaacgtcc ggaacggaca tccagacctg ctgctgctga cgggggatcc
     1081 cggcgtcggc aagagtcgcc tgctcaccga ggtcgttcga caggcggcgg ccgacgggtg
     1141 ggccgttgcg tcgagtcggt gcttcagcac ctcaggggtg gcgttgtcgc cggttgccga
     1201 ctggctgcgg tcaccggagg tggctgccgt ccgaccgtcg ctcgaccccg tctgggagtc
     1261 ggaggtggag cgactggtgc cgacaggtgg tggaggcgat cgtgatggga cggacgttct
     1321 cgaccgcagt ggtcaggcgc tggccgacac ctggcagcgg catcgcttct acgagggcct
     1381 gtgccaggcg ctgctgtcgg tggaccgacc gctgctcctg acgctggacg acctccactg
     1441 gtgtgaccag cagacgctgg catggatcgc cttccttctc cgactggcgg ccgaccgtcc
     1501 ggtgttcgtg ctggccacgg cccgagccga cgaactgcgg cacagcgaac tgcgccgtac
     1561 ggtgaccgac ttcagctccg ccggtttgct gacggagcac tcggtcgagc cgttgaccct
     1621 cgaggcgacg acagcgctcg cggcgcgcat gttcggacgc cccctcgaac cctcggacgc
     1681 agcgttgctg caggcggcgt cgggtggcta cccgctgtac gtcgtcgagg ccgcgcgcgc
     1741 ctcggcggcg gtcggcgggg acgccgaggt gagcgagtcc gggctcgggg tcgtgcttcg
     1801 acgcaggctc gccgagctga gttccgatgc acagcgcacg gttgcgctcg cgggagccat
     1861 cggccgcgac tttcggctgg acctgctcgt tcaagcgagc gaccaggacg aggtcactgt
     1921 cgcacgtgcg gtcgacgagc tctggcggcg ccgcgtactc caccaggtcg gccgtgggta
     1981 cgacttctcg cacgacctgc tgcgagacga ggcgtacgcg ctggtcgacc cggcgcagcg
     2041 ctggctcgaa caccgtcgag tagccaacgc cctcacggtc ctgaacgccg acgatctcga
     2101 cgccgtggca gcccagctcg cggagcagca cgagcgcggc ggacggcccg aggaggccgt
     2161 gcccttctat ttccgcgctg ccgtcgaggc tgcccgcgtc tacgccaacg ccgaggcggt
     2221 gcggctcttc cggcgttgcc tcgacatcgt ggcggagctc ccgaccagtc cggagcgcga
     2281 ccagcgcgag ctcgacgtac ggcaggcgat gtcggccccg ctgaacgccc tgtacggcta
     2341 cgcgcatcac gatctccggc acacgttcga gcggggcgtg gcgctcgccg agtcgctcgg
     2401 ccgcgtcgac cgccagctgt ccagcctggt cggactgtgg gcaaccacgt tcgtgaaggg
     2461 agacatcgac ggctcgcacc acatcgcgac cagggcgatc gcgttggccg accgggtgcc
     2521 tgcgcttgcc aaccaggccc acttcagcat ggggggcacg gccgtgagcc tcggtcgccc
     2581 agagctcgcg gtggagcagc tcgatcttgc ttgcgacctt tccgagggcg gaatctcgtt
     2641 gagtgttggt tcgcgtcctg agatccacgc ccgtgcctgg tccgcgcacg ggtactggct
     2701 gatgggcgac gacgtccgcg ccgactcagc ctgtcgtcag gcagtgtccg acgcgcgtct
     2761 gctcagtcac ccgtactcgc tggccatcgc cctctcctac gcgagcatca ctgcccagct
     2821 gcgtggtgac atgacgctgc tcgacgatag tctcgctgag ctcaccgacc tgtgcgagcg
     2881 gcacagctat gcgtactacc gcgagtgggc gttgattctg agcggctggc gcgccggcgg
     2941 cgaccgcggt ctcgcgcagg ccaagctcgg catcgcccac ctgtcgtcga gcggagcgct
     3001 cactcgtatg ccgtactggt tgtcgctgct ggcagactcg caacgcggca tcggcgccga
     3061 cgcggacgcc gacgccagtc tggacgctgc ctggactgcc gcctctcaac gcggcgacag
     3121 gtggtggctg cccgaggtgc tgcgggcgcg ggcgaagacg aagccgccgg atactgcggc
     3181 gaagatgctg cgccgcgcag aaaagactgc gcaagcccag tcgagtccgg tcctcgcgca
     3241 gcgttgtcgc gacgatctct cggcactcga gggcgtttcg cggcagccga gccgtgtgga
     3301 gccaggcgtt cgcgcacgca gtatcgctga cgaacgctga ccgaacgccg ccgccctagc
     3361 gtgacggtgg aactcagatc ccttgcgaaa ggacccttat gtcctccacc gtcgaaacag
     3421 agcaaccagc atccgcttcc gacctgccgc gccagcgcgg cgacgtgccc ttcgacacgc
     3481 tcgccgagca gttgtacgga agcgtcatca cgccggacga cccggagtac cacacggcac
     3541 gagccgtcta caacggcatg atcgaccgcc acccggccgc catcgcccgc tgcaagaact
     3601 ctgccgatgt cgtcgcctgc gtcaacttcg gacgtgagca cggcctcacc gtctccgtcc
     3661 gtggcggcgg gcacaacgcc ggcggactcg gcgtcgccga cgacgcactc gtgatcgacc
     3721 tttcggcgat gcgctccacc accgtgaacc cgacagagca caccgtgcgg gtggatggag
     3781 gctgcacctg gggcgacgtc gacaacgcca ccgttccgtt cgggatggcc acaccgagcg
     3841 gcgtcgtcag cacgaccggc gtgggtggcc tgactctggg tggtggcatc ggcaacctca
     3901 cccggagctt cggactcgcg atcgacaacc tgttgtcggc ggacgtcgta ctcgccgacg
     3961 gccggttggt gacggcgaat gagaagagtg agccggacct gttctgggcg ctgcgaggag
     4021 gcggtggcaa cttcggcgtc gtcacgtcgt tcaccttccg ctgcaacgag ctcggcgacg
     4081 cgggcacggt cgtggccggt cccgtgctgt atgacatcgc ggacaccgca gacgtgctgc
     4141 gctggtaccg cgagctgctg ccgtcactac cggaggagct caacggctgg ttcgcgacgc
     4201 tcacgattcc gggtggcccg ccgttccccg aggagctgtg ggggcgcaag gcgtgcggca
     4261 tcgtgtggtg ctacaccgga cctggtgagc gggccgacga ggtgctcgcg ccgatccgtg
     4321 acttcggctc cccgctgctg gtcgggctgg ctccgatgcc gttctcggcc ctccagagcg
     4381 cgttcgaccc gctgctccct cccgggctgc agtggtactg gcgggcagac atcgccgagg
     4441 agatcaccga cgaggcgatc gccatccatt ccaagtacgg cgaacagctg ccgaccgcgc
     4501 tctcgcagat gcacatgtat cccatcgacg gtgccgcgtc tcgcggctcg gctgacgaga
     4561 cggcgttcgc gtaccgcaac ggcggctggt cgaccgtgat ggtcggcgtc gaccccgacc
     4621 cggccaacaa ggacctgatc tccgactggg cgaaggcgta ctacgacgag ctgcacccga
     4681 cgacgaccgg cggtgcgtac gtgaacttca tgatggacga gggcgaggac cgggtgaagg
     4741 cgtcgtaccg cgacaactac gagcggctca ccgaggtcaa gaagacgtac gaccccgaca
     4801 acttcttcca cgtcaaccag aacattcggc ctgcgaaaca gagctgacga cccaacgggg
     4861 gagtctcgcg ggaacgtgac ggttccgcga gaccgggtcg ctcgtcgccg ggccggccga
     4921 ggatgtcttg agggggagac atccccggtc ggcccggcga ctgtccgttc caccggtgcg
     4981 gttggggttt gcgatgttta cagcccaaaa ggcgtgtaaa cagcgcaaac ctccgatggg
     5041 cacagtgggt ttgccgtctg aacatcgcaa aacaccgggt gctcagccgc ggatgtccga
     5101 aacccgcttg tcacacacca gccaaaaggg acggaacgcc ggcccaccgg ctgagcaggc
     5161 acgaactccc cgctcttggc tgccagccta ggtcagccga agccgacgga
//
LOCUS       DATBFZ010000377         1675 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_6367268, whole genome shotgun sequence.
ACCESSION   DATBFZ010000377 DATBFZ010000000
VERSION     DATBFZ010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6367268"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="VH419_11405"
     CDS             <1..320
                     /locus_tag="VH419_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020574605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4162 domain-containing protein"
                     /protein_id="HEY7044264.1"
                     /translation="DRGHLVAQGTPAELKRQVPGSYVRLQFTDVADLQRAALALTGST
                     KDDADLVLRVPSDARAASLRALLNQLHERSIDAEEFSVHTPDLDDVFLALTGRTSTEV
                     KAS"
     gene            317..1066
                     /locus_tag="VH419_11410"
     CDS             317..1066
                     /locus_tag="VH419_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012918672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY7044265.1"
                     /translation="MSTDSTIMLRRNFKHTVRNPTAVFNAVLFPIVIMLMFVYVLGGA
                     FDVGPDVDYIDYATPGIIVMAVAYGLSATSMAVNSDMAKGIINRFKVMDISRGAVLTG
                     HVVASMLRNLVAIAAIIAVAFLIGFRPSAGPLDWLGVVGLIVLVTFATGWLTVALGLA
                     AKTPESAGLATVPLIMLPFLSSAFVPADTMSVGVRQFAEYQPFTPIIETVRGLLNGAP
                     SAGDAIAALAWSIGIAVVGYLWALATFERRS"
     gene            1078..>1675
                     /locus_tag="VH419_11415"
     CDS             1078..>1675
                     /locus_tag="VH419_11415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HEY7044266.1"
                     /translation="MSETTQQARESRAVMRSARYAAPQPITAIVSVAGARVHIAASDR
                     TDTVVQVEPVNQASRTDMKVAARTKVDFTGGQLTVKTTTSGKKHGSVAITIDLPNGSN
                     LVAYLAHSSVHADGSLADCELHVASDRVRLDRVGTLQANVGAGEVAVGHVAGPAKIES
                     RSGQINIEHASADLELSNGSGAFDIERVDGNVTVMTGSG"
BASE COUNT          281 a          579 c          541 g          274 t
ORIGIN      
        1 tcgaccgggg ccacctggtc gcgcagggca ctcccgccga gctcaaacgg caggtgcccg
       61 gcagctacgt ccggctccag ttcaccgacg tcgccgacct ccagcgggcg gcactggccc
      121 tgaccggctc gaccaaggac gacgcggacc tcgttctacg ggttccgagc gacgcccgtg
      181 cggcgtccct gcgggctctg ctgaaccagc tgcacgaacg ctcgatcgac gccgaggagt
      241 tctcggtcca cacccccgac ctcgacgacg tcttcctggc cctgaccggc cgcaccagca
      301 cggaggtgaa agcatcatga gcaccgactc gaccatcatg ttgcgtcgca acttcaaaca
      361 caccgtccgg aacccgacag cggtgttcaa cgcggtcctg ttcccgatcg tgatcatgct
      421 gatgttcgtc tacgtgctgg gtggtgcctt cgacgtcggt cctgacgtcg actacatcga
      481 ctacgcaaca ccggggatca tcgtgatggc cgtcgcctac ggcctgagcg caacctcgat
      541 ggccgtgaac tccgacatgg cgaagggcat catcaaccgg ttcaaggtca tggacatctc
      601 gcgtggcgcc gtcctgaccg gacacgtcgt cgccagcatg ctgcgcaacc tggtcgccat
      661 tgccgcgatc atcgcggtgg ccttcctgat cggtttccgt ccctcggcag gaccgctcga
      721 ctggctcgga gtggtcggcc tcatcgtgct ggtcacgttc gcgaccggct ggctgaccgt
      781 cgcgttggga ctggccgcca agactccgga gtcggcgggt ctggccacgg tgccgctgat
      841 catgctgccg ttcctgagca gcgcgttcgt gccggccgac acgatgagcg taggtgttcg
      901 ccagttcgcg gagtaccaac ccttcacgcc gatcatcgaa accgtgcgcg ggcttctgaa
      961 cggtgcgccg tcggccggtg acgcgattgc cgctctcgcc tggagcatcg ggatcgccgt
     1021 cgtcggatac ctgtgggcgc tcgccacgtt cgagcgccgc tcgtgaccac cgccgcgatg
     1081 tccgagacga cgcagcaggc acgcgagagc agggcagtca tgcggagcgc gaggtacgcc
     1141 gcaccacaac cgatcaccgc catcgtgtcg gtcgccggcg cgcgggttca catcgccgcc
     1201 agcgaccgga ccgacaccgt ggtgcaggtc gaacccgtga accaggcgag ccggaccgac
     1261 atgaaggtgg ctgcccggac caaggtcgac ttcaccggcg gtcagctcac ggtcaagacg
     1321 accacgtcgg gcaagaagca cggctcggtt gccatcacga tcgacctccc caatggctcg
     1381 aacctcgtcg cgtacctggc gcactcgagt gttcacgctg acgggtcgct tgccgactgc
     1441 gagctgcacg tggcgtcgga ccgggtccgt ctcgaccgcg tcggcacact tcaggcaaac
     1501 gtcggggctg gagaggtggc ggtcggtcac gtcgccgggc cggccaagat cgagagtcgg
     1561 tccgggcaga tcaacatcga gcacgcctca gccgatctcg agctcagcaa cggcagcggg
     1621 gcgttcgaca tcgagcgggt cgacggcaac gtcaccgtca tgacgggcag cggcg
//
LOCUS       DATBFZ010000378         2459 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_257215, whole genome shotgun sequence.
ACCESSION   DATBFZ010000378 DATBFZ010000000
VERSION     DATBFZ010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257215"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..884)
                     /locus_tag="VH419_11420"
     CDS             complement(<1..884)
                     /locus_tag="VH419_11420"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEY7044267.1"
                     /translation="MLGSLPRWIRVLCWSGVGLVVIAIALFSVSVWTVHRSFPKTSGT
                     LTLKGLDAKVTVLRDSHGIPQIYADTSNDLFFAQGYVQAQDRFFQMDFRRHVTAGTLS
                     SLFGTDALETDEVVRTMGWRRVAEQELSLISPETRRYLEAFSNGVNAYLTDHDGATLS
                     LEYAVLGLDGVQYTPDPWTPVDSLAWLKAMAWNLGSNIDDEVDRSLESVSLTRRQIGE
                     LYPAYPYATNRPIVNQGAIVDGVFEQDARRSGTRLPQRPGFLSRSTEALLRTKHATHA
                     LSALLGSTGGAIGSNAWAV"
     gene            1027..1353
                     /locus_tag="VH419_11425"
     CDS             1027..1353
                     /locus_tag="VH419_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012923332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HEY7044268.1"
                     /translation="MPVYQYACTECAEQLEVRQSFTDDALTVCPACGGPLRKVLSAVG
                     VVFKGSGFYRTDSRTSSGEKASAASNGAGDSKDSSSTGKESTKTGSSATTSNDSSSST
                     PAAATG"
     gene            1427..2053
                     /locus_tag="VH419_11430"
     CDS             1427..2053
                     /locus_tag="VH419_11430"
                     /inference="COORDINATES: protein motif:HMM:NF020250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAF domain-containing protein"
                     /protein_id="HEY7044269.1"
                     /translation="MTDLRGRLTVARRWLVRFRRPLAALCAGGCVLAVVHVVAPPAPA
                     TTSVLVAAHRIAGGTTLSADDVVMVARPTGAVPPTAFRSLRQALGRTVAGPIDADEVL
                     TPARLVGPRLAAGFGPDEVACPVRLADADMVDLLSVGDRVDVYAPTAGGREASRIALD
                     APVVAIPVPSDDMSRSEGGLVVLAVSSPVAGRLAQQAAKAPLSVTLRP"
     gene            complement(2059..>2459)
                     /locus_tag="VH419_11435"
     CDS             complement(2059..>2459)
                     /locus_tag="VH419_11435"
                     /inference="COORDINATES: protein motif:HMM:NF013869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MscL family protein"
                     /protein_id="HEY7044270.1"
                     /translation="RCAMIRDMLKGFRDFILRGNLVELAVAFIMAAAFTMVVNATVAL
                     ILDIVGKIGGKADFSSYAPGGVGIGAWITAVISFVTMAAVVYFVIVLPYQTARATLSR
                     SGTDEPAVVPEDIALLTEIRDLLADRPRQG"
BASE COUNT          369 a          836 c          856 g          398 t
ORIGIN      
        1 acggcccacg cgttcgaccc gatcgcaccg ccggtgctgc cgagcagcgc cgagagggca
       61 tgcgtcgcgt gcttggtccg cagcaaggcc tccgtgcttc ggctgaggaa gcctggccgc
      121 tgcggaagtc gcgtgccgct cctgcgcgca tcctgttcga acacgccgtc gacgatggcc
      181 ccctggttca cgatgggccg gttggtggcg tacggatatg ccgggtagag ctccccgatc
      241 tggcgacgag tgagcgagac cgactccagg gacctgtcga cctcgtcgtc gatgttgctg
      301 cccaggttcc aggccatcgc cttgagccag gcgagcgagt ccaccggtgt ccacgggtcg
      361 ggggtgtact gcaccccgtc caggccgagc acggcgtact ccagcgacag cgtggctcca
      421 tcgtgatcgg tgaggtacgc gttgacgccg ttcgagaacg cctcgaggta gcgccgggtc
      481 tcaggggaga tcagcgagag ctcctgctcg gcgacccgcc gccagcccat cgtccggacg
      541 acctcgtcgg tctccagcgc gtccgtgccg aacagcgagc tcaaggtgcc ggccgtgacg
      601 tgccgccgga agtccatttg gaagaaccgg tcctgggcct ggacgtaccc ctgggcgaag
      661 aacaggtcgt tgctggtgtc ggcgtagatc tgcgggatcc cgtggctgtc acgcagcacg
      721 gtcaccttcg cgtcgaggcc tttcagcgtc agggttccgc tggtcttcgg gaacgagcgg
      781 tgcacggtcc acaccgacac ggagaacagc gcgatcgcga tgacgacgag cccgacgccg
      841 gaccagcaga gcacgcgaat ccagcgtggg agggacccga gcacgcgccc cattgtgcat
      901 ggcgggctca cgctccgctc aggacgcttt gcttctgtcg cccggctgcc gcacaatggg
      961 gatggttggc agtctcccgg gaagagtgct agccctggga agcgacgaac gcctgaggga
     1021 gcatccgtgc ccgtctacca atacgcctgc accgagtgcg cggagcagct cgaggtgcgc
     1081 cagagcttca ccgacgacgc gctgaccgtc tgccctgcct gtggcgggcc gctccgtaag
     1141 gtgctcagcg cggtcggtgt cgtgttcaag ggctcgggct tctaccgcac cgacagccgg
     1201 accagttccg gtgagaaagc atccgcggcg tcgaacggcg cgggtgactc caaggactcc
     1261 tcgagcaccg gcaaggagag cacgaagacc ggctcctccg cgaccacgtc gaacgactcg
     1321 agcagcagta cgccggctgc cgccacgggc tgacgcgtcg ctccatcctg caggcacctg
     1381 tggacggcgg ccccggggac gttggttggt ttcctagcct gcctggatga ccgacctgcg
     1441 tggccgtctg accgttgccc ggcggtggct cgtccgcttc cgacgtccgc tggctgccct
     1501 ctgcgcgggc ggttgcgtgc tggccgtggt ccacgtcgtc gccccaccag ctcccgcgac
     1561 gacgtcggtg ctggtcgccg cacaccggat cgcgggaggc acgaccctgt ccgccgacga
     1621 cgtggtgatg gtggcccgac ccacgggtgc ggtgccgccg acggctttcc gctcactccg
     1681 gcaggccctc ggccggaccg ttgccgggcc gatcgacgcg gatgaagtgc tgactcccgc
     1741 gcgactggtc ggaccgcggc tggccgccgg gttcggcccg gatgaggtcg cgtgcccggt
     1801 gcggctcgct gacgccgaca tggtcgacct gctgtcggtc ggtgaccgcg tcgacgtgta
     1861 tgcgccgacc gccggcggcc gggaggcatc ccggatcgcg ctggatgcgc cggtcgtcgc
     1921 gatccctgtc ccatcggacg acatgagccg gtccgagggt gggctcgtcg tactggcggt
     1981 ctcgtcaccg gttgctggtc gcctcgccca gcaggcggcg aaggcgccgc tgtcggtcac
     2041 gcttcgaccg tgaggccgtc agccttgacg gggccggtcg gcgagcaggt cgcggatctc
     2101 ggtgagcagc gcgatgtcct ccggtacgac cgccggctcg tccgtgccac tccgcgaaag
     2161 ggttgctcgc gccgtctggt acggcagcac gatgacgaag tacacgaccg ccgccatcgt
     2221 gacgaagctg atcactgccg tgatccaagc gccgatgccg acaccgccgg gcgcgtacga
     2281 cgagaagtcg gccttgccac cgatcttgcc gacgatgtcg aggatcaggg cgaccgtcgc
     2341 gttcaccacc atcgtgaacg ccgcggccat gatgaacgcg acggcgagtt caacgagatt
     2401 cccgcggagg atgaagtctc tgaagccttt gagcatgtcc cgaatcatgg cgcaccgga
//
LOCUS       DATBFZ010000379         1816 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_3737683, whole genome shotgun sequence.
ACCESSION   DATBFZ010000379 DATBFZ010000000
VERSION     DATBFZ010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3737683"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="VH419_11440"
     CDS             complement(<1..364)
                     /locus_tag="VH419_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008363146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase cytochrome b
                     subunit"
                     /protein_id="HEY7044271.1"
                     /translation="MSLDTSKIADTNRGTAATAKKPNKAGAVAGWADDRLSVGTLTKV
                     FARKVFPDHWSFMLGEIALWSFVVLLLTGTFLTFWFTPSMSESTYEGSYTQLRGVQMS
                     DAYASTLHISFDVRGGLLM"
     gene            complement(367..1473)
                     /locus_tag="VH419_11445"
     CDS             complement(367..1473)
                     /locus_tag="VH419_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012920408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEY7044272.1"
                     /translation="MSDNLPETTHDGHGPVTVAEPIADPGLPAHEWRPTDVDPKAEKR
                     AERQVAALFGIAVVASILFIVSYIVFQVGDNPDTIGNLGASNLALGLCLAVTLLAMGA
                     GMIQWARKLMGDHEIVEMRHPASSSQEDREAAVAILQTGLEESGLGRRPLIRNSLLAA
                     GGVLGLLPIVMLRDLGPLPGDKLYHTIWDMNTWTPEMQAWWKTQNRTLQRVFRDVVGT
                     PIKVTDLEIGDLVNAEPEVLFAVDPETKQPIVEGTDLLVAKSKAAVILHRMLPSDIVS
                     AQTRNWSVQGIVCYSKICTHVGCPISLNERTTHHLLCPCHQSTFDLADSGRVLFGPAA
                     RPLPQLQITVDDEGYLAAVRDFTEPVGPSFWERG"
     gene            complement(1470..>1816)
                     /locus_tag="VH419_11450"
     CDS             complement(1470..>1816)
                     /locus_tag="VH419_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HEY7044273.1"
                     /translation="CHNFEGSGGAMPHGGYAPKIRGVEPKYIFEAMLTGPQAMDKFSN
                     GNLSIEEKRDIVAYLQSLQDNPQYGGFGMGGIGPVSEGLFAWIVGIGSLVGVATWIAA
                     HTARSSKERAEP"
BASE COUNT          286 a          585 c          644 g          301 t
ORIGIN      
        1 gcatcagcag gccgccgcgc acgtcgaagc tgatgtgcag cgtcgaggcg taggcgtcgg
       61 acatctggac gccccgcagc tgcgtgtacg agccttcgta cgtcgactcc gacatcgacg
      121 gagtgaacca gaacgtgagg aacgtgccgg tcagcagcag caccacgaag gaccacagcg
      181 cgatctcgcc gagcatgaac gaccagtggt cggggaagac cttgcgggcg aagaccttgg
      241 tgagcgtgcc cacgctgagc cggtcgtcgg cccacccggc gacggcgccg gccttgttcg
      301 gcttcttggc cgtggccgcg gtgccgcggt tggtgtcggc gatcttgctg gtgtccagcg
      361 acatggtcag cctcgctccc agaaactcgg cccgactggt tcggtgaagt cgcgtactgc
      421 tgccaagtat ccctcgtcgt cgacggtgat ctggagctgc ggcagcggcc gggcggccgg
      481 gccgaacagc acccgaccgc tgtcggccag gtcgaacgtc gactggtggc aggggcagag
      541 caggtggtgg gtggtgcgct cgttcaacga gatcgggcaa cccacgtggg tgcagatctt
      601 ggagtagcag acgatgccct ggaccgacca gttgcgggtc tgcgccgaga cgatgtcgct
      661 cggcagcatc cggtgcagga tcaccgcggc cttggacttc gccaccagca ggtcggtgcc
      721 ctcgacgatc ggctgcttgg tctcgggatc gacggcgaag agcacctcgg gctcggcgtt
      781 caccaggtca ccgatctcga ggtcggtgac cttgatcggg gtaccgacga cgtcacggaa
      841 gacgcgctgg agcgtgcggt tctgggtctt ccaccaggcc tgcatctccg gcgtccacgt
      901 gttcatgtcc cagatcgtgt ggtacagctt gtcgcccggg agcggcccga ggtcgcggag
      961 catgacgatc ggcagcaggc cgaggacacc gccagcggcg agcagcgagt tacggatcag
     1021 cggacggcgt ccgagaccag actcctcgag cccggtctgc aggatggcga ccgcggcctc
     1081 gcggtcctcc tgcgaggacg acgcggggtg gcgcatctcg acgatctcgt ggtcgcccat
     1141 cagcttgcgt gcccactgga tcatgccggc gcccatggcc agcagcgtca ccgccaggca
     1201 gaggccgagc gcgaggttgc tggcgccgag gttgccgatc gtgtcgggat tgtcaccgac
     1261 ctggaagacg atgtagctga cgatgaagag gatcgacgcc acgacggcga tcccgaacag
     1321 cgccgccacc tgacgctccg cgcgcttctc ggccttcggg tcgacgtcgg tcggccgcca
     1381 ctcgtgggcc ggcaggccgg ggtcggcgat gggctcggcg acggtgacgg gaccgtgccc
     1441 gtcgtgcgtg gtctcgggga ggttgtcgct catggttccg ccctctcctt cgacgaccgt
     1501 gccgtgtgtg ccgcgatcca ggtggccacg cccaccagcg agccgatgcc gacgatccag
     1561 gcgaacaggc cctcactgac agggccgatg ccacccatcc cgaagccgcc gtactgaggg
     1621 ttgtcctgga gtgactgcag gtaggcgacg atgtcgcgct tctcctcgat cgacaggttg
     1681 ccgttcgaga acttgtccat ggcctgcggg ccggtgagca tcgcctcgaa gatgtacttc
     1741 ggctcgacgc cacggatctt cggcgcgtag ccgccgtgcg gcatggcgcc gccggagccc
     1801 tcgaagttgt ggcagg
//
LOCUS       DATBFZ010000380        15223 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_5056544, whole genome shotgun sequence.
ACCESSION   DATBFZ010000380 DATBFZ010000000
VERSION     DATBFZ010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 15223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15223
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5056544"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            468..1700
                     /locus_tag="VH419_11455"
     CDS             468..1700
                     /locus_tag="VH419_11455"
                     /inference="COORDINATES: protein motif:HMM:NF024825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY7044274.1"
                     /translation="MTTHRRWMSVAAVAAVAALIMAGCGSKGSSDEGTESGKLTIGAA
                     MSLTGELAREGALTKEGYQLCQQKVNAAGGVDVGGDKLDVEIKFQDDTSEPDTAAQLV
                     DQFNDDGIKLILSSYGSANTQAQAAVIERNGQVMADSAGADDEIFAQGYTRTFAVLSP
                     ATEYAASMVDAIDELASPKPKSFAFLSADDGFSKTGAKGGMAEAKKLGYDVIPAEYFP
                     EGSTDVSSSVNKVKSDSPDVVIGTVHLEEGVAIVKQSHELGLTPMAFGETVAPPTPDF
                     ADALGSLANGVLGSSQWTQTTVGQDQYFGTAKEYAADIEKTYGHPADYHDAEASAACL
                     AMVMAVEKAGSTDPDKVREALAGLDTESFFGKIKFDDTGKNVYKPMSVIQVQDGKVVT
                     VWPKEAAEASLIWPATKQ"
     gene            1739..2626
                     /locus_tag="VH419_11460"
     CDS             1739..2626
                     /locus_tag="VH419_11460"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY7044275.1"
                     /translation="MTAFLQATVYGLLQGGLLALIAVGFSLVWGVMNIVNLAHGAFVI
                     LGAYLAWELHAHAGLPPLLGMFVVGAALFCLGYVLQYGLINLVVNAPIFITLLLTFGL
                     SLLIVQGMNIVFTANYRSIQTPYAGDSFALGDVRVPVGRLLAFVIAVLITVALVVLIN
                     RTRIGIAIIATGMDRGAARLMGIPARHVYALTFALSAGLAGIAGTMVGTIDTFTPADA
                     GGFTLLSFVIAVLGGLGSMYGALVGGLVVGLVESWAGLYFSGTWINATAFLVLVVVLV
                     VRPSGLLGRAYSAARVEAA"
     gene            2626..3681
                     /locus_tag="VH419_11465"
     CDS             2626..3681
                     /locus_tag="VH419_11465"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY7044276.1"
                     /translation="MDKTVEEEAAPAQETSATVRRAPPPTWRRLSPWWVLLLALLAML
                     GLQSQLYIGQERTVTAIFMFVALAQAWNLIGGFTGYACFGQVVFFGLGGYITAAAMVH
                     WNVSFWVALALSGIGAAAFAVIIGLPLLRLKGHYFAIATLGVAEGMREVVTNLDDITG
                     GGAGITIPTVGEDATTAYLGNDGFYLLFLAVAVVSVAVALLVSRSRFGFALRAIHQDE
                     EAAAAMGINTTRTKVLVFALSGLITGVVGGCYAFQQVVIFPDRLFDVELTVLMVVMVV
                     IGGSGTVLGPVVGAVGLQYLSEYLRHNYTDLHIFILGAIIIVAVVFMPQGIVNYVREA
                     RQSGDFSLLANVRRYRL"
     gene            3693..4475
                     /locus_tag="VH419_11470"
     CDS             3693..4475
                     /locus_tag="VH419_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017934724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY7044277.1"
                     /translation="MLETRGLGRRFGGLQAVADVSVAVPEGEILGVIGPNGAGKSTFI
                     NLVTGQLRPSAGQVLVRGKDMTGARPWAMASAGVARTFQIMKPFRGMTVRENVAVGIL
                     YGSDRPGTMVKALERADEVLDGVGLAGREEQVPSELSAADSRRLELAKALALDPQLLL
                     LDEVMAGLRPNEITPSLDLIRRLRDRGLTILVVEHVMKAIREVSDRVLVIHQGRVLTS
                     GEPDDVLSDPRVVEAYLGTRYAQRHAEDDAPDRQGASDARDH"
     gene            4459..5151
                     /locus_tag="VH419_11475"
     CDS             4459..5151
                     /locus_tag="VH419_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY7044278.1"
                     /translation="MLEITDLTAGYGRLEVLWDVGLRVDEREVVALVGPNGAGKTTLL
                     RVISGMVPAQSGTMTFRDVSLVGRSIENVVRLGIAHVPEGRRLFPGLSVRDNLRLGGW
                     RTKNADLDPVLEMFPALKDRLHQIAGSLSGGEQQMCAIGRGLMGRPDLMLIDELSLGL
                     APVVVDELIERLPAIADSGTSVLLVEQDVESALRVAARAYVLETGRIVVSGVAEDVLT
                     DSRVQDSYLGLG"
     gene            5292..6308
                     /locus_tag="VH419_11480"
     CDS             5292..6308
                     /locus_tag="VH419_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012919772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY7044279.1"
                     /translation="MTDVARLAGVSHMTVSRVLNGHPNVRESTRDRVAAAIEQLEYRR
                     NSAARALVTNRTQTLGVVAFDTTLYGPASTLFGIEQAAREAGYFVTIVSLKQITRESV
                     AQAVEHLTSQSVDGLVVIAPQEEAVEALTAVPAGLPVVAVEGGSAPDLPLVCVDQAEG
                     ATQATQHLLDLGHETVWHVAGPPDWLEAVARRDAWRATLERAAATVRDPLPGDWSPRS
                     GYEAGKVLAQRDDVTAVFVGNDQMALGLLRAFGEAGVEVPGQVSVVGFDDIPESAYFS
                     PPLTTVHQDFTEVGRGCIRVLLDQIVGEVEAPVGTPRLVVPTELVARASTAPPAHHRR
                     GASA"
     gene            6305..7969
                     /gene="araB"
                     /locus_tag="VH419_11485"
     CDS             6305..7969
                     /gene="araB"
                     /locus_tag="VH419_11485"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011753874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HEY7044280.1"
                     /translation="MTQPEDRYVVGIDFGTLSGRAVVVRVSDGAELASRVHDYPHGVV
                     DRTLPTTGQRLPPDWALQVPGDYIDVLRNAVPKAVAVAGVDPDKVVGIGTDFTACTIL
                     PTRADGTALSELPEYAGRPHAYPKLWKHHAAQRQADRINALARDLGESWLARYGGLIS
                     SEWEFAKGLQLLEEDADVYAATERFVEAADWIVWQLCGSYVRNTCTAGYKGIRQDGGY
                     PSREFLARLNADFAGFVEDKLDHPIGELGDRAGGLTEQAAAWTGLPAGIAVAVGNVDA
                     HVTPPAANAIRPGQMVAIMGTSTCHVMNGEVLREVPGMCGVVDGGITAGYWGYEAGQS
                     GVGDIFAWFVDTSVPAEYADEANRRGVDLQTYLGELAAAQRVGEHGLVALDWHSGNRS
                     VLVDHELSGIVVGQTLATRPEDVYRALLEATAFGTRTIIEAFDAAGVPVEELVVAGGL
                     LKNRFLMQMYADVTGLPLSVIASEQGPALGSAIHAAVAAGAYPDVPAAAAAMGRSEPG
                     AYTPDPERAKAYDALYAEYAMLHDYFGRGGNDVMHRLRDIRRKAVR"
     gene            7966..8643
                     /locus_tag="VH419_11490"
     CDS             7966..8643
                     /locus_tag="VH419_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009080657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEY7044281.1"
                     /translation="MTLSVDSQAVAALRRSVCDLHAELVRNRLVAWTSGNVSGRLPGT
                     DLFAIKPSGVSYDDLTPQAIVLCDLSGEVVDGELSASSDTAAHAYVYRQLPEVGGIAH
                     THSTYATAWAARGEPIPCVLTAMADEFGGEIPVGPFALVGDDSIGRGVVATLQGSRSR
                     AVLMQNHGVFTVGKDARDAVKAAVMCEDVARTVHVARSGGEPIPIPQPDVDLLHARYQ
                     NIYGQRK"
     gene            8646..10145
                     /gene="araA"
                     /locus_tag="VH419_11495"
     CDS             8646..10145
                     /gene="araA"
                     /locus_tag="VH419_11495"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011753876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HEY7044282.1"
                     /translation="MPRDVECWFLTGSQGLYGEDTLEQVATQSRGIAEALDRSDDVPV
                     RVVWKPVLTDADAIRRACVDAGASDSCIGVIAWMHTFSPAKMWIAGLEALRVPLLHLH
                     TQANVELPWATIDMDFMNLNQAAHGDREFAHLQTRLGVARKTVSGHVSDPTVTARVGS
                     WMRAALGLAALRSLKLVRFGDNMRDVAVTEGDKVEAQRRLGVSVNTFGVNDLVAAVAA
                     AAESEVDLLAKEYERAYRVVPELRRGGDRHGSLIEAARIEAGLRRFLVEGGYRAFTTN
                     FEDLGGLQQLPGIAVQRLMADGYGFGAEGDWKTAILVRAFKAMAEGLPGGTSFMEDYT
                     YHLVPGQERILGAHMLEVCPTIAAEQPSCEVHPLSIGGRDDPVRLVFDAAPGPAVVVG
                     LADLGDRFRLVANEVELVRPDEPLPKLPVARAVWRPLPDFATSTESWLTAGGPHHTVL
                     SAAVGAETVVDLAEMLRIELVLIDGSTTTRGLADQLRWNQAYHRLAQGL"
     gene            10289..11338
                     /locus_tag="VH419_11500"
     CDS             10289..11338
                     /locus_tag="VH419_11500"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEY7044283.1"
                     /translation="MRTQAMTGRLTAVLATLVLVAACGGSSDDDGNDESGSTTDAASA
                     SGGFCEGTKLVFFPGGTQGGGFETVVYNGARAAEKAFGPDVAYEWSDWDPSKMVSQFQ
                     EAMATNPDGIAVMGHPGDDAFDPLIDDARSQGILVTVMNTELPKAEAKYAADGTGYVG
                     AVLYDAGSALAKETIARGNLQSGDRVFVWGLKSQPGRGERTKGITDTLEDAGMQVDYL
                     EIDDATNADPAAGVPAFTGYVSSHPDVKAIVIDHGNLTSTIPTYLEAANLGADDVYAA
                     GFDLSPATVKGVQDGYVDLVIDQQQYLQGFLGILQLCLTHQYGFSGLFVNTGGGFVDK
                     SNIDAIAPLVKDQIR"
     gene            11356..12264
                     /locus_tag="VH419_11505"
     CDS             11356..12264
                     /locus_tag="VH419_11505"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY7044284.1"
                     /translation="MNEPETGEQNARRSPEQQPGANGRAPVDKGAEERLALELVDVTK
                     SFGGVGVLHGVSLQLRKGEVLGLLGDNGAGKSTMIKIVMGVHHPDTGRLLFDGKQVDN
                     LTVEKARRLGVEAVYQERALAEQLSLWRNIFMGRPVRGPLGFLDVGTMRRVTAQLMGE
                     SMGFTSAALTPDTNVTGLSGGERQGLAIVRALHFEADIIILDEPTMGLSLGETDKLLR
                     FVGDIRGRGKSAIFIDHNIFHVYSVADHIVVLDRGRVAGDFPKSRYSLDELTSIMREL
                     AATGQFAEPDRPTPGDHPHRTSSGGS"
     gene            12266..13255
                     /locus_tag="VH419_11510"
     CDS             12266..13255
                     /locus_tag="VH419_11510"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY7044285.1"
                     /translation="MALLEMGQMSPKAFATRWASQIGITVAFALVWLTFIILAPETFL
                     DSRIYVSFGAITPYFALPALLLTMVIVCGDIDLSFPSVMAMGMLGFIAVEQNNGSVFL
                     AVLAALAVGAAAGLFNGLIVTIVGVPALVVTIGTQFLFRGITLVAVSGKSYPLVETRD
                     TPTFNVLVGKLHGVPMQFIWLVAFAVGTWLLLYRHRLGHNAHVIGDNRHAAELMGIPI
                     RRTRVILFVLTGMAAALSGVMNSLQVSNFYPGTLGTGFFLPALAAVFVGGTSVFGGRG
                     TVWGTFIGAFMIGGITAGIVAVGLTQYWTNLIYGAIVLIAISIHAVLQRRFER"
     gene            complement(13321..14160)
                     /locus_tag="VH419_11515"
     CDS             complement(13321..14160)
                     /locus_tag="VH419_11515"
                     /inference="COORDINATES: protein motif:HMM:NF015709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family acid phosphatase"
                     /protein_id="HEY7044286.1"
                     /translation="MNRKFSLSAGLCAVAAAATLALVGPGTAVGHRQGPVPAPPTNPT
                     RADQIQNIDQVRTAIKGYYGDTVTDQVDPVPNNVDGADVVLHTFDQDGSYAHEVAGIE
                     RRASWYLAHRAHRHHFDGKPALIFDIDDTTLNTFNYEIYSNFAYNPTTNGYFVNAAVF
                     PAVPGMPELVSRAAAQGYEVFFLTGRPETQRAGTATNLTNAGYGTPAADHVFLKDQTL
                     PWLSSCTPGCTSAQYKALTRQHLIEDLGFDIVANFGDQPSDFTGGFEDTTVKLPNPMY
                     YLP"
     gene            complement(14336..14983)
                     /locus_tag="VH419_11520"
     CDS             complement(14336..14983)
                     /locus_tag="VH419_11520"
                     /inference="COORDINATES: protein motif:HMM:NF018446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HEY7044287.1"
                     /translation="MPPDSTAHSETTETTTRLEAFSDGVFAIAITLLVLELRVPEVEP
                     GQLWSALWDEWPQFAAYFTSFLIIGIMWVNHHSMFRQIGRADRPLIFLNLLLLLWMAM
                     LPFPTELVARYLDEGGSNAHVAAAVYSANLTLAAIAFSMLWWHAVRGGLVDRVMSPRE
                     QWRSIWRFSIGTVVYAATIALSFVSAPLTLAIQFLIAAYYAFEQLHTRASARQPA"
     gene            15043..>15223
                     /locus_tag="VH419_11525"
     CDS             15043..>15223
                     /locus_tag="VH419_11525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044288.1"
                     /translation="MATIQIREIPEDVYEVIRLRARRSGRSIQSYMRDVVVDFASHPT
                     MDEALAAMDAARAASN"
BASE COUNT         2429 a         5048 c         5311 g         2435 t
ORIGIN      
        1 cgacattctt gaccgccgga ggtcggcagc gaagccaaag ccaggcagca acctcttccg
       61 cttatcggtg ccaagaatcc tcaccacagc ggagtgagct tgatctcgac agtgatgagc
      121 agcgggctcc gtcggtcgag cctgtcgaga cccgcccggt gatctcgaca agctcgatca
      181 gcgggttgcg gcggtcgagc ctgtcgagac cccgcccggt gatgtcgaca gtgttgatca
      241 gcggtcacca cgacattctc gactaccgga ggtcggcagc gaagcccaag ccaggcacca
      301 atccctcccg gtcatcgctg ccaagaaccc tcaccacagc ggacaggacc gtgatctcga
      361 cgaagctcga tcagcgggta ctggcgggag tggctcgccg tgcggggtga tgcggaactg
      421 gttatggtgc gaagtggaca gcctcaatcg cacccaagag gtgaaccatg accacgcacc
      481 gtcgttggat gtccgttgcc gccgtcgccg cagtcgcggc gctgatcatg gccggctgtg
      541 gctccaaggg atcctcggac gaaggcacgg agtcggggaa gctcacgatc ggcgccgcca
      601 tgtcgctcac cggagagctc gcccgtgaag gcgccctcac caaggagggc taccagctct
      661 gccagcagaa ggtgaacgcc gccggtggtg tcgacgtcgg tggtgacaag ctcgacgtcg
      721 agatcaagtt ccaggacgac acgtccgagc cggacacggc ggcccagctg gtcgaccagt
      781 tcaacgacga cggcatcaag ctcatcctca gctcctacgg ctcggcgaac acgcaggcac
      841 aggccgcagt gatcgagcgc aacggtcagg tgatggccga ctccgccggt gccgacgacg
      901 agatcttcgc ccagggctac acgcgcacct tcgccgtcct ctcgcccgcc acggagtacg
      961 ccgcctcgat ggtcgacgcg atcgatgagc tcgccagccc gaagcccaag agcttcgcgt
     1021 tcctctccgc cgacgacggg ttttcaaaga cgggcgccaa gggcggcatg gccgaggcga
     1081 agaagctcgg gtacgacgtg atccccgcgg agtacttccc agagggaagc accgacgtga
     1141 gctcctcggt caacaaggtc aagtccgaca gccccgacgt cgtgatcggc accgtccacc
     1201 tcgaggaggg cgtcgcgatc gtcaagcagt cgcacgagct cgggctcacc ccgatggcat
     1261 tcggagagac ggtggcaccg ccgaccccgg acttcgccga cgccctcggg tcgctggcca
     1321 acggcgtgct cggctcgagc cagtggacgc agacgacagt gggccaggac cagtacttcg
     1381 gcaccgccaa ggagtacgcc gccgacatcg agaagaccta cggtcacccg gccgactacc
     1441 acgacgccga ggcgagcgcg gcctgcctgg ccatggtgat ggcggtcgag aaggccgggt
     1501 cgaccgaccc ggacaaggtc cgcgaggcgt tggccgggct ggacaccgag tccttcttcg
     1561 gcaagatcaa gttcgacgac accggcaaga acgtctacaa gccgatgtcg gtgatccagg
     1621 tgcaggacgg caaggtcgtc acggtctggc cgaaggaagc cgcggaggcg agcctgatct
     1681 ggcccgcgac caagcagtga ccgcgccctg acctcgacga ccggatggtg attcgcccgt
     1741 gaccgcgttc ctccaggcca cggtctacgg gctgctccag ggcgggctgc tcgcgctgat
     1801 cgcggtcggc ttctcgctgg tgtggggcgt gatgaacatc gtcaacctgg cccacggcgc
     1861 gttcgtcatc ctcggtgcct atctcgcctg ggagctgcac gcgcatgccg gtctgcctcc
     1921 gctgctcggc atgttcgtcg tcggcgccgc cctgttctgt ctggggtacg tcctgcagta
     1981 cgggctgatc aacctcgtcg tgaacgcgcc gatcttcatc accctgctgc tgacgttcgg
     2041 actgtccctg ctcatcgtgc aggggatgaa catcgtcttc acggccaact accgcagcat
     2101 ccagacgccg tacgccggcg acagcttcgc gctcggcgac gtccgggtcc cagttggtcg
     2161 gctgctcgcg ttcgtcatcg ccgtcctcat cacggttgcg ctcgtcgtgc tcatcaatcg
     2221 cacgcgcatc ggcatcgcga tcatcgccac cggcatggac cgcggtgccg cgcgactgat
     2281 gggcatcccg gccagacacg tctacgcgct gacgttcgcg ttgtccgcgg ggcttgccgg
     2341 catcgcgggc acgatggtgg gcacgatcga cacgttcacg cctgctgacg ccggtggctt
     2401 cacgctgctc tcgttcgtga tcgcggtgct gggcgggctc ggcagcatgt acggcgcgct
     2461 ggtgggtggc ctcgtcgtcg ggttggtcga gtcgtgggcg ggtctctact tctctgggac
     2521 ctggatcaac gccaccgcgt tcctcgtgct cgtcgtggtc ctcgtcgtac gcccatccgg
     2581 cctgctcgga cgcgcgtact cagccgctcg ggtggaggcg gcctgatgga caagaccgtc
     2641 gaggaggagg ccgctcccgc acaggagacg agtgccaccg tccggcgcgc gccgccgccg
     2701 acgtggcgtc gactgagtcc atggtgggtg ctgctgctcg ccctgctggc gatgctcggc
     2761 ctgcagagcc agctctacat cggtcaggag cggaccgtca ccgcgatctt catgttcgtc
     2821 gcgctggctc aagcctggaa cctgatcggc gggttcaccg ggtacgcctg cttcgggcag
     2881 gtcgtgttct tcggtctcgg cggctacatc accgcggccg cgatggtgca ctggaacgtc
     2941 agcttctggg tcgcgctggc gctcagcggt atcggcgctg ctgcgttcgc cgtgatcatc
     3001 ggactgcccc tgctgcggct caagggccac tacttcgcca tcgccaccct cggcgtggcc
     3061 gaagggatgc gtgaagtggt cacgaacctg gacgacatca ccggcggcgg cgccggcatc
     3121 acgatcccca cggtcggcga ggacgccacc acggcgtacc tcgggaacga cggcttctac
     3181 ctgctgttcc tggcggtcgc ggtggtgtcc gtggccgtgg cgttgctggt ctcgcgcagc
     3241 cgcttcgggt tcgcgttgcg ggcgatccat caagacgagg aagccgccgc cgcgatgggc
     3301 atcaacacga cgcggacgaa ggtgctggtc ttcgcgctgt cggggctgat caccggcgtg
     3361 gtcggtgggt gctacgcctt ccagcaggtc gtgatcttcc ccgaccggct cttcgacgtc
     3421 gagctgaccg tgctcatggt ggtgatggtg gtcatcggcg gtagcggcac ggtgctcggc
     3481 ccggtcgtgg gcgcggtcgg tctgcagtac ctgtcggagt acttgcgaca caactacacc
     3541 gacctgcaca tcttcatcct cggggcgatc atcatcgtcg cggtcgtctt catgcctcag
     3601 ggcatcgtca actacgtccg cgaggctcga caaagtggcg acttctcgct gctcgcgaac
     3661 gtgcggcggt accggttgtg aacgcgcccc cggtgctgga gacaaggggc ctcggacggc
     3721 ggttcggcgg cttgcaggcg gtggccgacg tctcggtcgc cgtgcccgag ggcgagatcc
     3781 tcggggtgat cggcccgaac ggcgccggca agagcacctt catcaatctc gtgacgggcc
     3841 agctcagacc ctcggcagga caggtcctgg tgcggggcaa ggacatgacg ggtgcgcgcc
     3901 cgtgggcgat ggcgtcggcc ggggtggcgc gcacgttcca gatcatgaag ccgttccgtg
     3961 gcatgacggt tcgggagaac gtcgcggtcg ggatcctgta cgggtccgat cgtccgggga
     4021 cgatggtcaa ggcgctcgag cgggccgacg aggtgctcga cggggtgggc ctcgccggac
     4081 gcgaggagca ggtgccctcg gagttgtcgg cggcggactc gcgccgactc gagctggcca
     4141 aggcgctggc gctggacccg cagctcctgc tgctggacga ggtgatggcc ggactgcggc
     4201 ccaacgagat cacgcccagc ctcgacctga tccgtcggct ccgagaccga ggcctgacca
     4261 tactggtcgt cgagcacgtg atgaaggcga tccgggaggt cagcgatcgc gtcctggtca
     4321 tccaccaggg gcgagtgctc acctcgggag agccggacga cgtactgtcc gatccgcgcg
     4381 tcgtcgaggc atacctgggc acccgctacg cgcagcggca cgccgaggac gacgccccag
     4441 accgacaagg tgccagtgat gctcgagatc actgacctga ccgccggata cggccgcctc
     4501 gaagtgctct gggacgtcgg cctgcgcgtc gacgagcggg aggtcgtcgc cctcgtcgga
     4561 ccgaacggcg ccggcaagac gacgctgctg cgggtgatct cgggaatggt gccggcacag
     4621 tcgggcacga tgaccttccg tgacgtgagc ctggtcggcc ggtccatcga gaacgtggtc
     4681 cggctcggca tcgcgcacgt ccccgagggc aggcggctgt tccccggcct cagcgtccgc
     4741 gacaacctgc gcctcggcgg ctggcggacg aagaacgccg acctcgatcc tgtgttggag
     4801 atgttcccag cgctcaagga ccggctgcac cagatcgccg gcagcctctc cggcggcgag
     4861 cagcagatgt gcgccatcgg ccgcggcctg atgggccggc cggacctgat gctgatcgac
     4921 gaactgtccc tcggtctggc cccggtggtc gtggacgagc tgatcgagcg gctgcccgcg
     4981 atcgccgact ccggcacctc cgtgctgctg gtggagcagg acgtcgagtc ggcgctgcgc
     5041 gtcgcggccc gcgcgtacgt gctcgagacg ggtcgcatcg tggtctccgg cgtcgcggag
     5101 gacgtgctca ccgacagccg ggtgcaggac agctatctcg gcctcggctg acgaccttgc
     5161 cgagttgtca gacgctggtc gagctatagg tcgaccagcg gctgacaact cggtgttagc
     5221 cttaacatca cgtccgccta gaccgtgcgg gggagcccgt gtccagcccg tacaagcaac
     5281 gcccgccgtc gatgaccgac gtcgctcggc tcgcgggggt gtcgcacatg accgtctccc
     5341 gggtgctcaa cggccatccg aatgtccgcg agtcgacccg agaccgggtc gcggccgcca
     5401 tcgagcagct ggagtaccgc cgcaactcgg ccgctcgggc gctggtcacc aatcggacgc
     5461 agacgttggg cgtggtcgcc ttcgacacga cgctctacgg ccctgcaagc acgctgttcg
     5521 ggatcgagca ggcggcccgc gaggccggct acttcgtgac catcgtcagc ctcaagcaga
     5581 tcacccgcga gagcgtggcc caggccgtcg agcacctgac gagccagtcg gtcgacgggc
     5641 tggtcgtgat cgccccgcag gaggaggcgg tcgaggccct caccgcggtc ccggccggcc
     5701 tgcccgtcgt cgcggtggag ggtggcagcg cgccggacct gcccctggtg tgcgtggacc
     5761 aggccgaagg cgcaacgcag gccacccagc acctcctcga cctaggtcac gagaccgtgt
     5821 ggcacgtcgc cgggccaccg gactggctcg aggcggtggc ccggcgcgac gcctggcggg
     5881 ccactctcga gcgagccgct gcgaccgtgc gtgacccgct gccaggggac tggagtccca
     5941 ggtccggcta cgaagcaggc aaggtgctcg cccagcgcga cgacgtgacc gcggtgttcg
     6001 tgggcaacga ccagatggcg ctgggactgc ttcgcgcgtt cggggaggca ggcgtggagg
     6061 ttcccggcca ggtgagtgtg gtcggcttcg acgacatccc cgagtcggcg tacttctccc
     6121 cgccgctgac gaccgtgcac caggacttca ccgaggtcgg tcgcgggtgc atccgggtgc
     6181 tgctggacca gatcgtcggc gaggtcgaag cgcccgtcgg tacgccgcgg ctcgtcgtcc
     6241 ccaccgagct ggtcgccagg gcgagcaccg ccccgccagc acaccaccgg agaggagcat
     6301 ccgcgtgacc caacccgagg accggtacgt ggtcggcatc gacttcggga cgttgtccgg
     6361 ccgagcggtc gtcgtgcgcg tcagtgacgg ggccgagctc gccagccgcg tgcacgacta
     6421 cccgcacggt gtcgtggacc gcacactgcc gacgaccggc cagcgactcc cgccggactg
     6481 ggcgcttcag gtgcccggtg actacatcga cgtgctgcgc aacgccgtgc ccaaggcggt
     6541 cgctgtggcc ggcgtcgacc cggacaaggt cgtcgggatc ggcaccgact tcaccgcctg
     6601 caccatcttg cccacccggg ccgacggcac cgcgctgtcg gagctgccgg agtacgccgg
     6661 ccgcccccat gcatacccga agctgtggaa gcaccacgcc gcacaacggc aggccgaccg
     6721 catcaacgcg ctcgcccgcg acctcggcga gtcgtggctg gcgaggtacg gggggttgat
     6781 ctcgagcgag tgggagttcg ccaagggcct ccagctgctc gaggaggacg ccgacgtcta
     6841 cgccgcgacc gagcgcttcg tcgaagccgc cgactggatc gtgtggcagc tgtgcgggtc
     6901 gtacgtgcgc aacacctgca cggccggcta caagggcatc cgccaggacg gcggctaccc
     6961 gagcagggag ttcctcgcca ggctcaacgc cgacttcgcc gggttcgtcg aggacaagct
     7021 cgaccacccc atcggtgagc tcggtgaccg cgccggtggt ctcaccgagc aggcggcggc
     7081 gtggaccggg ctgccggccg gcatcgcggt cgcggtcggc aacgtcgacg cgcacgtcac
     7141 cccgcccgcc gcgaacgcca tccggcccgg ccagatggtc gccatcatgg gcacctcgac
     7201 ctgccacgtg atgaacggcg aggtgctccg tgaggtaccc ggcatgtgcg gggtggtgga
     7261 cggcggcatc acggccggct actggggcta cgaggcaggg cagagcggcg tcggcgacat
     7321 cttcgcctgg ttcgtcgaca cgtcggtgcc cgccgagtac gccgacgagg cgaaccgtcg
     7381 cggcgtcgac ctgcagacct acctcggtga gctggcggcg gcacagcgcg tgggggagca
     7441 cgggctggtc gcgctggact ggcacagcgg caaccgctct gtgctcgtcg accacgagct
     7501 gtccgggatc gtggtcgggc agacgctcgc cacccggccc gaggacgtct accgcgcgct
     7561 gctcgaggcg accgcgttcg ggacgcgcac gatcatcgag gcgttcgatg cggccggcgt
     7621 gccggtggag gagctggtcg tcgcgggcgg tctgctcaag aaccgcttcc tcatgcagat
     7681 gtacgccgac gtgaccgggc tgccgctgtc ggtgatcgcc tcggagcagg gccctgcgct
     7741 ggggtcggcg atccacgccg cggtcgccgc cggtgcctac cctgacgtcc ctgcggcggc
     7801 ggccgcgatg ggcaggtcgg agcccggggc gtacacaccc gatccggagc gcgccaaggc
     7861 gtacgacgcg ttgtacgccg agtacgccat gctgcacgac tacttcggcc gaggcgggaa
     7921 cgacgtgatg caccggctcc gcgacatccg gcggaaggcg gtgcgatgac gctgtcggtg
     7981 gactcgcagg cggtcgccgc gttgcggcgc agcgtctgtg acctgcatgc ggagctggtg
     8041 cgcaaccggc tggtcgcctg gacgtcgggg aacgtgtccg ggcggctgcc cgggacggac
     8101 ctgttcgcga tcaagcccag cggagtctcc tacgacgacc tgacgccgca ggcgatcgtg
     8161 ctgtgtgacc tctccggcga ggtcgtcgac ggcgagctgt ccgcatccag cgacaccgcc
     8221 gcgcacgcct acgtgtaccg gcagctgcca gaggtcgggg ggatcgcaca cacccattcg
     8281 acctacgcga cggcatgggc ggcgcgcggc gagcccatcc cctgcgtgct cactgcgatg
     8341 gccgacgagt tcggcggtga gatccccgtc gggccgttcg cgctcgtcgg cgacgactcg
     8401 atcggtcgcg gcgtcgtcgc gacgctgcag gggagcaggt cgcgggccgt cctcatgcag
     8461 aaccacggtg tcttcaccgt cggcaaggac gcacgcgacg cggtcaaggc cgcggtgatg
     8521 tgcgaggacg tggcccgcac cgtccacgtc gcgcgctcgg gtggcgagcc gatcccgatc
     8581 ccgcagccgg atgtcgacct gctccacgcg cgctaccaga acatctacgg ccagaggaag
     8641 tgaccatgcc acgagacgtc gagtgctggt tcctgaccgg aagccagggt ctgtacggcg
     8701 aggacacgct ggagcaggtg gccacgcaat cacgcggcat cgccgaggcg ctcgaccgct
     8761 cggacgacgt accggtgcgg gtcgtctgga agccggtgct gaccgatgcg gacgccatcc
     8821 gacgcgcctg cgtcgatgcc ggcgccagcg actcgtgcat cggggtcatc gcgtggatgc
     8881 acacgttctc accggccaag atgtggatcg ccggcctcga ggcgctccgc gtgccgctgc
     8941 tgcatctgca cacccaggcc aacgtcgagc tgccgtgggc gaccatcgac atggacttca
     9001 tgaacctcaa ccaggcggcg cacggcgacc gtgagttcgc gcacctccag acgcgcctag
     9061 gtgtggcgcg caagacggtg agcggacacg tcagcgaccc gacggtgacc gcgcgcgtgg
     9121 ggtcatggat gcgcgccgca ctcggcctcg ccgcgctgcg ttcgctcaag ctggtgcggt
     9181 tcggcgacaa catgcgcgac gttgcggtga cggagggcga caaggtggag gcccaacgcc
     9241 ggctcggcgt ctcggtcaac accttcggcg tcaacgacct ggtcgcggcc gtcgccgccg
     9301 cggcggagtc ggaggtcgac ctgctggcca aggagtacga acgggcgtat cgcgtcgtac
     9361 ccgaactgcg tcgcggtggc gaccgccacg ggtcgctgat cgaagcggct cggatcgagg
     9421 ccggtctgcg ccggttcctc gtcgaagggg gctaccgggc gttcaccacc aacttcgagg
     9481 acctcggagg gctgcaacag ctgcccggca tcgccgtaca acggctgatg gccgacggct
     9541 acgggttcgg cgccgaaggc gactggaaga cggccatcct ggtacgtgcg ttcaaggcga
     9601 tggccgaagg tctgcccggc ggcacgtcgt tcatggagga ctacacctac cacctcgtgc
     9661 cggggcagga acggatcctc ggtgcgcaca tgctcgaggt ctgcccgacg atcgcggccg
     9721 agcagccttc gtgcgaggtc catccgctgt ccatcggtgg tcgcgacgac ccggttcggc
     9781 tggtgttcga cgcggcgccc gggccggcag tcgtggtcgg gctcgccgac ctgggcgacc
     9841 ggtttcggct cgtggccaac gaggtcgagc tcgtacgccc ggacgagccg ctgccgaagc
     9901 tcccggtggc gcgggcggta tggcgaccgc tgccggactt cgcgacttcg accgagtcgt
     9961 ggctcaccgc cggcggaccg caccacaccg tgctctcggc ggcagtcggg gccgagacgg
    10021 tcgtcgacct ggccgagatg ctgcgcatcg agctggtgct catcgacgga tcgaccacaa
    10081 cgcgcgggct ggcggaccag ctgcggtgga accaggccta ccaccggctc gcccagggcc
    10141 tctgaaccgg atcagacagg tgatcgacat gcgcggcgag gctcggtcgg tagcgggagg
    10201 gaagagttta cggtgggctc ttgtgagcgc taacaatctc ggcctaaggt ttcgaggagc
    10261 gacgaccgct cctgaaggga gtgcagggat gcggacacag gcaatgacgg gacggttgac
    10321 tgcggtgctg gccacgctgg tactggtggc agcctgcggc ggaagttcgg acgatgacgg
    10381 gaacgacgag tccggttcga cgacggatgc cgccagcgcg tctggcgggt tctgcgaggg
    10441 gaccaaactc gtgttcttcc ccggaggtac ccaaggcggc ggcttcgaga ccgtggtcta
    10501 caacggcgcg cgggccgctg agaaggcctt cgggccggac gtggcgtacg agtggtctga
    10561 ctgggatccc agcaagatgg tcagccagtt ccaggaggcc atggccacca acccggacgg
    10621 catcgcggtc atgggccacc ccggtgacga cgccttcgat ccgctgatcg acgacgcccg
    10681 cagccagggc atcttggtca cggtgatgaa caccgagctg ccgaaggcgg aggcgaagta
    10741 cgccgccgac ggcactggct acgtcggtgc ggtgctgtac gacgccgggt ctgccctggc
    10801 caaggagacc atcgcacgtg gcaacctcca gtccggagac cgcgtcttcg tctggggcct
    10861 gaagtcccag ccggggcggg gtgagcggac caagggcatc accgacacgc tcgaggacgc
    10921 cggcatgcag gtcgactacc tcgagatcga cgacgccacc aacgccgacc cggccgccgg
    10981 tgtcccggcg ttcaccgggt acgtgtcgtc ccacccggac gtcaaggcga tcgtcatcga
    11041 ccacggcaac ctcacgtcga cgatcccgac gtacctcgag gcggcgaacc tgggcgccga
    11101 tgacgtgtac gccgcgggct tcgacctctc accggccacc gtgaagggag tccaggacgg
    11161 ctacgtcgac ctggtgatcg accagcagca gtacctccag ggtttcctcg gaatcctgca
    11221 gctgtgcctg acgcaccagt acgggttcag cggcctgttc gtcaacaccg gtggtggctt
    11281 cgtcgacaag tcgaacatcg acgcgatcgc gccgctggtc aaagaccaga tcaggtgatg
    11341 ggtggcggca gccgcgtgaa cgagccggag accggcgagc agaacgctcg tcggtctccg
    11401 gagcaacaac ccggtgccaa cggtcgggca ccagtcgaca agggcgccga ggagcggctc
    11461 gccctcgagc tcgtcgacgt caccaagtcg ttcggaggcg tcggcgtgct ccacggggtg
    11521 agtctccagc tgcgcaaggg cgaggtgctc ggtctgctcg gcgacaacgg tgccggcaag
    11581 tcgacgatga tcaagatcgt catgggggtg caccaccccg acaccggccg cctgttgttc
    11641 gacgggaagc aggtggacaa cctcaccgtc gagaaagcac gccgactcgg cgtcgaggcg
    11701 gtctaccagg agcgcgccct ggccgagcag ctttcgctgt ggcgcaacat cttcatgggc
    11761 cggccggttc gcgggccgct cgggttcctc gacgtcggca cgatgcgccg cgtcaccgcc
    11821 cagctcatgg gcgagtcgat ggggttcacg tcggccgcgc tcacgcccga cacgaatgtc
    11881 accggactct ccggcggtga acgtcaaggc ttggcgatcg tgcgggcgct ccacttcgag
    11941 gccgacatca tcatcctcga cgagccgacg atgggcctgt cactcggtga gaccgacaag
    12001 ctgttgcggt tcgtgggcga catccgtggc aggggcaagt cggccatctt catcgaccac
    12061 aacatcttcc acgtgtactc ggtggcggac cacatcgtcg tactcgatcg gggccgggtc
    12121 gccggcgact tcccgaagag tcgctactcg ctggacgagc tgacatcgat catgcgcgag
    12181 ctggccgcga ccgggcagtt cgccgagcct gaccgcccca ctccggggga ccacccgcac
    12241 cgcacgtcgt cagggggctc gtgagatggc gctgctggaa atgggtcaga tgtctccgaa
    12301 ggcgttcgcg acgagatggg cgtcgcagat cggtatcacg gtggcgttcg ctctcgtctg
    12361 gttgacgttc atcatcctgg cacctgagac gttcctcgac agccgcatct acgtgtcgtt
    12421 cggtgccatc acgccgtact tcgccctccc cgccctgctg ctgacgatgg tcatcgtctg
    12481 tggcgacatc gacctgtcct tcccgtcggt catggcgatg ggcatgctcg ggttcatcgc
    12541 ggtggaacag aacaacggaa gcgtgttcct cgcggtgttg gcggcgctgg ccgtcggggc
    12601 ggcggcggga ctgttcaatg gcctcatcgt caccatcgtg ggtgtccctg ctctcgtcgt
    12661 gacgatcgga acgcagttcc tgttccgcgg tatcacgttg gtcgcggtga gcggcaagag
    12721 ctatccgctc gtggagaccc gcgacacccc cacgttcaac gtgctcgtcg gcaagctgca
    12781 cggcgtgccc atgcagttca tctggctggt tgccttcgcg gtgggcacgt ggctgctgtt
    12841 gtaccggcac cggctcggcc acaacgcgca cgtgatcggc gacaaccggc acgcggcgga
    12901 gctgatgggc attcccatcc ggcgcacccg ggtcatcctc ttcgtcctca ccggaatggc
    12961 ggcagcgctg tccggggtga tgaacagcct gcaggtgtcg aacttctacc ccggcacgct
    13021 cggtacgggc ttcttcctgc ccgcgctggc ggccgtcttc gtcggtggca cctcggtctt
    13081 cggcgggcgt ggcacggtct gggggacgtt catcggcgcg ttcatgatcg gcggcatcac
    13141 cgcggggatc gtggcggtgg gactgaccca gtactggacc aacctgatct acggcgcgat
    13201 cgtcctcatc gccatctcga tccacgccgt cctgcaacgc cgcttcgagc gctgatttgc
    13261 cgagttgtca gaagccagtg gacctatagg tccatgtact tctgacaact cggcacaacc
    13321 tcacggcagg tagtacatcg ggttcggcag cttgaccgtg gtgtcctcga aaccgccggt
    13381 gaagtcgctc ggctggtcgc cgaagttcgc gacgatgtcg aagccgaggt cctcgatcag
    13441 gtgctggcgg gtcagcgcct tgtactgcgc cgaggtgcag ccgggggtgc agctcgacag
    13501 ccacggcagc gtctggtcct tgaggaacac gtggtctgcc gccggagtgc cgtacccggc
    13561 gttggtgagg ttggtcgccg tgccggcgcg ctgcgtctcc ggtcgaccgg tcaggaagaa
    13621 cacctcatag ccctgagccg ctgccctcga cacgagctcg ggcatcccgg ggacggcggg
    13681 gaacaccgca gcgttgacga agtacccgtt ggtcgtgggg ttgtacgcga agttgctgta
    13741 gatctcgtag ttgaaggtgt tcaacgtggt gtcgtcgatg tcgaagatca gcgctggctt
    13801 gccgtcgaag tggtgccggt gtgcacggtg ggcgaggtac cagctcgcgc gacgctcgat
    13861 cccggcgacc tcgtgggcgt aggagccgtc ctggtcgaac gtgtgcagga cgacgtccgc
    13921 gccgtcgacg ttgttcggta ccgggtcgac ctggtcggtg acggtgtcgc cgtagtagcc
    13981 cttgatggcc gtccggacct ggtcgatgtt ctggatctgg tccgcgcggg tcgggttggt
    14041 cggcggggca gggacaggac cttgccggtg gccgacggcg gtcccgggac cgaccagggc
    14101 gagcgttgca gcggcggcca cagcacacaa gccggccgac agcgagaact tccggttcac
    14161 atttccacct tccctgagcg ggtcggagcc cacccgggtg agacgaacag gtcacgacgc
    14221 cggccggcaa gcgacttcga cagctctgcc cggcgaccag cgcccgggac agccaacatc
    14281 aagcaacgcg ctttgtccac catcgacggt tgcgcgcccg gcgcgacagc cgcgatcaag
    14341 ccggttgacg ggccgaggcg cgggtgtgga gctgctcgaa ggcgtagtac gccgcgatca
    14401 ggaactggat ggcgagggtc aacggcgcgg agacgaacga gagcgcgatg gtggcggcgt
    14461 acacgacggt gccgatcgag aaccgccaga tcgaccgcca ctgctcccgc ggtgacatga
    14521 cgcggtcgac cagtccgcca cggacggcgt gccaccacag catgctgaac gcgatcgctg
    14581 ccaaggtgag gttggcgctg tacaccgcgg ccgccacgtg ggcgttggag ccaccctcgt
    14641 cgagatagcg ggcgacgagc tcggtcggga acggcagcat cgccatccag agcaagagca
    14701 gcaggttcaa gaagatcagc ggacggtccg cccggccgat ctgccggaac atcgagtggt
    14761 ggttgaccca catgatcccg atgatcaaga agctggtgaa gtacgccgcg aactgcggcc
    14821 actcatccca cagcgccgac cacagctgac ccggctccac ttccggaacg cgcagctcca
    14881 gcacgagcag cgtgatcgcg atcgcgaaga cgccgtcact gaacgcctcg agccgagtgg
    14941 tggtctccgt ggtctcggag tgagccgtcg agtcgggagg cacgatgtca ggaataccag
    15001 atcccggcga cacgtacggc gctgtcgcgc taaagtagcg ctatggcgac gattcagatc
    15061 cgtgagatcc ccgaggacgt ctatgaggtg atccggctgc gcgcccggag atccggcaga
    15121 tcgatccagt cctacatgcg ggacgtggtc gtcgacttcg ccagccatcc gaccatggac
    15181 gaagcattgg cagccatgga cgcggcgcgt gcggcgagca acc
//
LOCUS       DATBFZ010000381         3008 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_2231741, whole genome shotgun sequence.
ACCESSION   DATBFZ010000381 DATBFZ010000000
VERSION     DATBFZ010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 3008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3008
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2231741"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..1502)
                     /locus_tag="VH419_11530"
     CDS             complement(33..1502)
                     /locus_tag="VH419_11530"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF037667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEY7044289.1"
                     /translation="MTRARTALLTAVALVVAVVAGSTGDGSTAAGTTEHTVSQASDLG
                     RGHVGLADVGVGDPYFPRDGNPGYHVRHYGIHVRYEPQTEVLTGSTTVTMRADAVLRT
                     LDLDLVLTATGVRVDGQPVHFRQTRHELTLTPRTPLHRGQVARVSVDYRGKPTRLRYD
                     GERPMVRTPTGAIAVGEPHSAAWWFPSNDHPSDKATFTTVLSVPRGNEGISNGALLRH
                     RDHGDRSVWRWRTGTPMAPYLAFAAFGQYDVERGHAGGRPYLYAFERGPAARSTAAHR
                     SLHSTPAILRWLSHVWGRYPYRQTGGVVPSRGLGFALENQTRPVYGLDMFTFGVDRGL
                     IAHELSHQWFGDRVTVRQWRNIWLNEGFATYTEWLWGHHTGGKSPQRQFLDTYHVFED
                     SSLFWNVRIGDPGPHRLFDWAVYERGAMTLQALRNRVGSRAFFTIARRWVHANHDGVG
                     STREFRRLAEHVSGERLGGLFHAWLYDGSKPAPTHANGL"
     gene            complement(1787..2986)
                     /gene="hppD"
                     /locus_tag="VH419_11535"
     CDS             complement(1787..2986)
                     /gene="hppD"
                     /locus_tag="VH419_11535"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020387100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HEY7044290.1"
                     /translation="MSETLTPEEVQADLTLDQLKQLVGLVEYDEEKDPFPVTGWDAIV
                     FVVGNATQVAHYYQSAYGMDLVAYSGPETGNRDHKAFVLRSGACRFVVKGGVAPDSPL
                     LDHHRAHGDGVFDISLEVPDVDKCVAQARQMGATVLREPEDVSDEYGTVRIGAIATYG
                     DTRHTLVDRSKYDGAYLPGYVARTTTVKRPDGAPRRLFQALDHVVGNVELGKMDDWVK
                     FYNGVMGFVNMAEFIGDDIATDYSALMSKVVANGNHRVKFPLNEPAVGKRKSQIDEFL
                     EFYQGPGAQHLALATNDILATVDAMRAQGVEFLSTPDSYYEDPELRARIGKVRVPIEE
                     LQGRGILVDRDEDGYLLQIFTKPIGDRPTVFFELIERHGSLGFGKGNFKALFEAIERE
                     QELRGNL"
BASE COUNT          478 a          995 c         1019 g          516 t
ORIGIN      
        1 cgatcgccgc ggacgagtgg tcgccgacga cgtcagaggc cgttggcgtg cgtgggagcg
       61 ggcttgctcc cgtcgtacag ccaggcgtgg aagagcccac cgaggcgctc gcccgacacg
      121 tgctcggcca accgacggaa ctcgcgggtg ctgccgacgc cgtcgtggtt ggcatgcacc
      181 cagcgccggg cgatcgtgaa gaaggctcgg ctgcccacgc ggttacgcag cgcctggagc
      241 gtcatcgctc cccgctcgta gacagcccag tcgaacaacc ggtgcgggcc gggatccccg
      301 atccgcacgt tccagaacag gctggagtcc tcgaaaacgt ggtaggtgtc gaggaactgg
      361 cgctgcggag acttgccccc ggtgtgatgg ccccacagcc attcggtgta cgtcgcgaag
      421 ccctcgttca gccagatgtt ccgccactgg cggacggtca cccggtcgcc gaaccactgg
      481 tgtgacagtt cgtgtgcgat gaggccgcga tcgacgccga aggtgaacat gtcgagcccg
      541 tagaccggtc gggtctggtt ctccaacgca aagcccaggc cgcggctcgg gaccacgcca
      601 cccgtctgcc gatacgggta gcgaccccag acgtgcgaga gccaccgaag gatcgccgga
      661 gtggaatgga gtgaacggtg tgccgctgtc gagcgggctg ccggacctcg ctcgaacgcg
      721 tagaggtacg ggcgaccgcc ggcgtggcca cgctcgacgt cgtactgtcc gaacgcggcg
      781 aacgcgaggt acggcgccat gggtgtgccc gtgcgccagc gccagacgga tctgtcgccg
      841 tggtcgcgat gcctgagaag ggcgccgttg ctgatcccct cgttgcctcg cggcacggag
      901 aggaccgtgg tgaacgtcgc cttgtcgctg ggatggtcgt tgctcgggaa ccaccaggcg
      961 gcgctgtgcg gctcaccgac cgcgatcgcg ccggtcggtg tccgcaccat cggccgctcg
     1021 ccgtcgtagc ggagcctggt cggcttcccg cgatagtcga cgctcacgcg ggcgacctgg
     1081 ccacggtgca gaggcgtccg cggcgtcagg gtgagctcgt gccgcgtctg ccggaagtgc
     1141 acgggctgcc catccacgcg gacaccggtc gcggtgagca cgaggtcgag gtcgagcgtg
     1201 cgaaggaccg cgtcggcgcg catggtgacg gtcgtggatc cggtcagcac ctcggtctgc
     1261 ggttcgtacc gcacgtggat gccgtagtgc ctgacgtggt agcccgggtt gccgtcgcgc
     1321 gggaagtacg gatcgcccac gccgacatcg gccagaccaa cgtgaccccg tccaaggtca
     1381 gacgcctgag agacggtgtg ctcggtcgtg ccggctgcgg tcgagccgtc accggtcgag
     1441 ccggccacca cggcaacgac aagagcaacc gccgtgagca gcgcggtgcg cgcacgcgtc
     1501 atcgaagggc tccagatctg gtcgtacgcc gcatcgacgg ccgtgggtga accgacaccc
     1561 tctcagaccg ggcaccgtgt cgagaaattt gtggcccgcg aagttgccag cgtcacgtcc
     1621 ggaaccggcg aaccgcggca tctgcggagg tggtttgtgt gatgagccga tggttcgcat
     1681 ggcaaaccca ctgtgcacat cggaggtttg cgatgtttac acgccttttg ggctgtaaac
     1741 atcgcaaacc cccaggcgac cgtggcaaac cgggttcgct actgcgctag aggttgccgc
     1801 ggagctcctg ttcgcgctcg atggcctcga acagcgcctt gaagttgccc ttgccgaaac
     1861 cgagcgaccc gtgccgctcg atgagctcga agaacaccgt cggtcgatcc ccgatcggct
     1921 tggtgaagat ctgtagcagg tagccgtcct cgtcccggtc gaccaggatg ccgcgacctt
     1981 gcagctcctc gatcggcacg cgcaccttgc cgatccttgc gcgcagctcc gggtcctcgt
     2041 agtaggagtc cggtgtgctg aggaactcca caccctgcgc cctcatcgcg tccaccgtgg
     2101 ccagaatgtc gttggtcgcc agcgccaggt gctgtgcgcc cgggccttga tagaactcca
     2161 ggaactcgtc gatctgactc ttccgcttgc cgaccgccgg ctcgttgagc gggaatttga
     2221 cccggtggtt gccgttcgcc acgaccttgc tcatcaaggc ggagtagtcg gtggcgatgt
     2281 cgtcgccgat gaactccgcc atgttcacga agcccatgac accgttgtag aacttcaccc
     2341 agtcgtccat cttgccgagt tccacgttgc cgaccacgtg gtcgagcgcc tggaacagcc
     2401 ggcgcggtgc gccgtccggc cgcttcacgg tcgtggtcct ggcgacgtag ccgggaaggt
     2461 aggcgccgtc gtacttcgag cggtccacga gcgtgtgccg agtgtcgccg tacgtggcga
     2521 ttgcgccgat ccggaccgtc ccgtactcgt cgctcacgtc ctccggctca cggagcacgg
     2581 tggcgcccat ctggcgggcc tgggcgacgc acttgtccac gtccggcacc tcgagcgaga
     2641 tgtcgaacac gccgtcgcca tgagcgcggt ggtggtccag cagcgggctg tcgggcgcga
     2701 cgccaccctt gacgacgaac ctgcacgccc cgctgcgcag cacgaacgcc ttgtggtcgc
     2761 ggttgcccgt ctccggcccg ctgtatgcga caagatccat gccgtacgcc gactggtagt
     2821 agtgcgcgac ctgggtggcg ttgccgacca cgaagacgat cgcgtcccaa cccgtgaccg
     2881 ggaacgggtc cttctcctcg tcgtactcga caagacccac cagctgcttg agctggtcca
     2941 gggtgaggtc ggcctgcact tcttcggggg tcaacgtttc actcatgggc gcatcctggt
     3001 gctggata
//
LOCUS       DATBFZ010000382         3295 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_3215380, whole genome shotgun sequence.
ACCESSION   DATBFZ010000382 DATBFZ010000000
VERSION     DATBFZ010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 3295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3295
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3215380"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..948
                     /locus_tag="VH419_11540"
     CDS             <1..948
                     /locus_tag="VH419_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020665123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEY7044291.1"
                     /translation="KVISRTIAYHGTTQGALSITGLPGLKSMFEPLVPSTFRVPNTNI
                     YRAPVHGDDPEAFGRWAADQIAVAIEDEGPETVAAVFLEPVQNSGGCFPPPPGYFQRV
                     REICDEYDVLLVSDEVICAFGRLGHMFGSERFGYQPDVITCAKGITSGYAPLGAMIAT
                     DRLFEPFTKGDASFAHGYTFGGHPVSTAVAMANLDVFEREHINEHVRANEDAFRNTLE
                     KLLDLPIVGDVRGAGYFYGIELVKDKVTKETFNDDESEKLLRGFLSKALYDAGLYCRA
                     DDRGDPVVQLSPPLICDQQHFDEMEQILRSVLTEAWTKI"
     gene            complement(1080..1556)
                     /locus_tag="VH419_11545"
     CDS             complement(1080..1556)
                     /locus_tag="VH419_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-opening dioxygenase LigA"
                     /protein_id="HEY7044292.1"
                     /translation="MTEQKVAGRVRLLALLVMIAGLILIVAGVGTYVTVSNKLSDQEI
                     TVSDDAAHFGGEHVDQPWEAYAQANVIAKHANEIAGGKTYAELPQDDPNRDTVMNASF
                     LQASLFTSVVAFGVAVMAFGLGVVFILIGWALRSVVRTPAPGNVSHTAPVEEERES"
     gene            1765..2436
                     /locus_tag="VH419_11550"
     CDS             1765..2436
                     /locus_tag="VH419_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease V"
                     /protein_id="HEY7044293.1"
                     /translation="MWPDTADSLVALQERLAVTTPSRWQPGGSRLAIGGCWVCFPRGN
                     TGRGGAGDPAWVAAVVMDGYRVVERWAGRGETAAAYQPGLLALRVGQLMEDAVRALDA
                     PPDVLLVDATAADHPRRAGLALHLGEVLGIPTIGLTHRPLLADGDWPADERGATSPVR
                     IGGTVVGCWMRTRPGTRPLVVHPGWRVDLDTAVEIVRRTTPRRRTPEPLRRARQLARR
                     TRATN"
     gene            complement(2527..>3295)
                     /locus_tag="VH419_11555"
     CDS             complement(2527..>3295)
                     /locus_tag="VH419_11555"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY7044294.1"
                     /translation="LLIGIGVLIYTFVPIAYVILLSFNKPSGRAATTEWNQFTWDNWS
                     TICEPDGMCQSLRLSFEVSITATIISTILGTLLAFGLVRHSFRGRGATNIVIFLPMAT
                     PEVVMGASLLAMFIAAGFNSLLGFTTIVIAHVLFCLSFVVVTVKARLVGMDARLEQAA
                     MDLYANEWQTFRLVTLPLAMPGIIAGALLAFSLSFDDFIVTNFTSGRDVTFPLFIYGA
                     KQRGFPPQVFVIGTLMFLISFIFVIAGEGWRRRRRSY"
BASE COUNT          558 a         1120 c         1096 g          521 t
ORIGIN      
        1 aaggtcatca gccgcaccat cgcctaccac ggcaccaccc agggggcgct gtcgatcacg
       61 ggtctaccgg gtctcaagtc catgttcgag ccgctggtgc ccagcacctt ccgggtcccg
      121 aacaccaaca tctaccgcgc gccggtccac ggcgacgacc ccgaggcctt cggtcgctgg
      181 gccgctgacc agatcgcggt ggccatcgag gacgaaggac ccgagaccgt cgcggccgtc
      241 ttcctcgagc cggtccagaa ctccggtggc tgcttcccgc cgccgccggg atacttccaa
      301 cgtgtccgcg aaatctgcga cgagtacgac gtcctgctgg tcagcgacga ggtcatctgt
      361 gcgttcggtc ggctcggcca catgttcggt tccgagcggt tcggctacca gcccgacgtg
      421 atcacctgcg cgaagggcat cacctccggc tacgcgccgc tgggtgcgat gatcgcgacc
      481 gaccggctgt tcgagccgtt cacgaagggc gacgcgtcgt tcgcccacgg ctacacgttc
      541 ggcggtcacc cggtctcgac ggcggtggcg atggccaacc tcgacgtctt cgagcgtgag
      601 cacatcaacg agcacgtgcg tgcgaacgag gacgccttcc gcaacacgct cgagaagctg
      661 ctcgacctgc cgatcgtcgg cgacgtccgt ggtgccggct acttctacgg gatcgagctg
      721 gtcaaggaca aggtgaccaa ggaaaccttc aacgacgacg agtccgagaa gctgcttcgc
      781 ggcttcttgt ccaaggcctt gtacgacgcc ggactgtact gccgcgccga cgaccgtggc
      841 gacccggtcg tccagctctc gccgccgctc atctgcgacc agcagcactt cgacgagatg
      901 gagcagatcc tgcgctcggt gctgacggag gcgtggacga agatctagga cctacccccg
      961 ggagctcggc tccccccttg gtggcggtgc gctccgcgag actcggctcg cagagcgcac
     1021 cgccgtcttg tccctcgtcg gtggcgaggt cagccgctca ggaccgaccg gacctccgat
     1081 cagctctccc tctcctcctc gacgggcgcg gtgtgcgaca cgttgcccgg cgcaggagtg
     1141 cggacaacag accgcagggc ccagccgatg aggatgaaca cgactcccaa tccgaaggcc
     1201 atcactgcca ccccgaaggc aaccaccgag gtgaagagcg atgcctggag gaacgaggca
     1261 ttcatcaccg tgtcgcgatt cggatcgtct tgcgggagct ccgcgtaggt cttgccgccg
     1321 gcgatctcgt tcgcatgctt ggcgatgacg ttcgcttggg cgtacgcctc ccacggctgg
     1381 tcgacgtgct ctcccccgaa gtgcgcggcg tcgtcggaga cggtgatctc ctggtcgctg
     1441 agcttgttgc tcacggtgac gtacgttccc actccggcaa cgatcaggat gaggccggcg
     1501 atcatgacaa ggagcgccag cagccgtact cgacccgcaa ccttctgctc tgtcatgggc
     1561 gacatccttc ccgtttgtcg ctctcgacgg caggaccgac acccccagtc gagtgattcc
     1621 ctgcgacctg ccacgggaac cagccagacc cacccctccc aaggcaggtc tgcctggcta
     1681 cccgtccgtc tggatggcta gccaggcaaa ccaccagcga cgcctgtccg gagcggcata
     1741 ccttcgtctg cggcaacatg tccggtgtgg cctgacaccg ccgactccct cgtcgcgctg
     1801 caggagcggc tggcggtgac gacgccatcg cggtggcaac cgggtggctc ccggctcgcg
     1861 atcggcggct gttgggtgtg cttcccgcgc gggaacacgg gccgaggcgg cgctggtgat
     1921 ccggcctggg ttgccgctgt cgtgatggac gggtatcgcg tcgtcgagcg ctgggccggg
     1981 cgcggcgaga ccgcggcggc gtaccagccg gggttgctcg cgttacgggt cggccagctg
     2041 atggaggacg cggtacgcgc cctggacgca ccgccggacg tcctcctggt cgacgccacc
     2101 gccgcggacc atccacgtcg ggccgggctg gcgctgcacc tcggtgaagt gctggggatc
     2161 ccgacgatcg gcctgaccca ccggccgctg ctcgccgacg gggactggcc ggccgacgag
     2221 cggggggcga ccagtcccgt ccggatcggc ggcacggtcg tcgggtgctg gatgcggacc
     2281 aggcccggta cgcgcccgtt ggtcgtgcac cccggctggc gcgtcgacct cgacaccgcg
     2341 gtcgagatcg tgcgccgtac gacgcctcgc cggcgtacgc cggaaccgct tcgacgggct
     2401 cgtcaactcg cccgccgtac ccgggccacc aactgacccg cgaattgcgg gaagctccgg
     2461 gtttttcggc cccgaaatcg cggaacttcc gcaattcgcg ggagcgcagc gcggctgggc
     2521 ggggggtcag taggacctgc ggcggcggcg ccagccctct ccggcgatga cgaagatgaa
     2581 cgagatgagg aacatcagcg tcccgatgac gaagacctgc ggcgggaagc cgcgctgctt
     2641 ggcgccgtag atgaacagcg ggaacgtgac gtcgcggcca gacgtgaagt tcgtgacgat
     2701 gaagtcgtcg aacgacaacg agaacgccaa gagcgcaccg gcgatgatgc cgggcatggc
     2761 cagcggcaac gtgaccagcc ggaacgtctg ccactcgttc gcgtagaggt ccatcgccgc
     2821 ctgctcgagc cgggcgtcca tgccgaccag ccgcgccttg accgtcacca cgacgaacga
     2881 caagcagaac agcacgtgcg cgatcacgat cgtggtgaaa ccgagcaggc tgttgaagcc
     2941 ggccgcgatg aacatcgcca gcagcgaggc acccatgacg acctcggggg tggccatcgg
     3001 caagaagatg acgatgttcg tcgccccccg cccgcggaag ctgtgtcgga ccagcccgaa
     3061 cgcgagcagc gtgccgagga tcgtggagat gatcgtcgcc gtgatgctga cctcgaagct
     3121 gagtcgcagc gactggcaca tcccgtccgg ttcgcagatc gtgctccagt tgtcccaggt
     3181 gaactggttc cactcggtgg tcgcggcccg tccggacggc ttgttgaacg acaaaaggat
     3241 gacgtatgcg atgggaacaa acgtgtagat cagcacgccg atcccgatga gcagg
//
LOCUS       DATBFZ010000383         5242 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_594655, whole genome shotgun sequence.
ACCESSION   DATBFZ010000383 DATBFZ010000000
VERSION     DATBFZ010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 5242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5242
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_594655"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            207..1139
                     /gene="deoC"
                     /locus_tag="VH419_11560"
     CDS             207..1139
                     /gene="deoC"
                     /locus_tag="VH419_11560"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020578688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEY7044295.1"
                     /translation="MSSPAALRRFLHGLPGVDQVGAEARAATLASRSIKTTAKAWALD
                     MAISMIDLTTLEGQDTTGKVRALCAKARQPDPADPSCPRVAAICVYPDMVATAKTALG
                     SSGVQVASVATAFPSGRAMLDVKLQDTRDAVAAGADEIDMVIDRGAFLSGDYQQVFDE
                     IVAVKQACARPGGGEHAHLKVIMETGELVTYDNVRRASWLSMLAGADFIKTSTGKVQP
                     AATMPVTLVMLEAVRDFREATGRMVGVKPAGGISTAKDAIKYLVMVNETAGEDWLTPT
                     WFRFGASRLLNDLLMQRTKLATGRYSGPDYFTLD"
     gene            1164..2609
                     /locus_tag="VH419_11565"
     CDS             1164..2609
                     /locus_tag="VH419_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020391978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY7044296.1"
                     /translation="MAFEYAPAPESRSIVDLKSSYGLFVNGEFVDGGGHAFKSVSPAT
                     EEVLAEIAEANETDVDLAVKAARRAATRVWSRMSGAERAKYLYRIARILQERSRELAV
                     LESLDNGKPIRESRDVDVPLVAAHFFYYAGWADKLEYALPGNRHSGPDPRPLGVAGQV
                     IPWNFPLLMLAWKIAPALACGNTVVLKPAETTSLTALLFAEICQQADLPPGVVNIVTG
                     AGETGRALVAHPDVDKVAFTGSTEVGKLIARTVAGSDKKVTLELGGKAANIIFDDAPV
                     DQAVEGIVNGIFFNQGHVCCAGSRLLVQESVYDEVLARLKRRMSTLRVGDPLDKNTDV
                     GAINSSEQLAKIRELSEIGETEGAERWSPKCDLPDRGFWFAPTIFTGVSQAHRIAREE
                     IFGPVLSILTFRTPAEAVEKANNTPFGLSAGVWTEKGSRILWTANQLRAGVVWANTFN
                     RFDPTSPFGGYKESGFGREGGRHGLEAYLAH"
     gene            2599..3486
                     /locus_tag="VH419_11570"
     CDS             2599..3486
                     /locus_tag="VH419_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020391977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY7044297.1"
                     /translation="MPTERSTARLDVRKTYKLYVGGAFPRSESGRSYVVNDAKGRFLA
                     NAAYASRKDARDAVTAARKAFPGWSGRTPYNRGQVVYRVAEVLEGRRDQFVDEVQRSE
                     GLTKRQAAGVVDATVDRLVWYAGWADKVAQVVGGTNPVAGPYFDFSIPEPTGVVAVLA
                     PQESSLLGLVSVLAPVVVTGNTCVVAASYERPLPAITLSEVLATSDVPGGVVNVLTGR
                     LADTAPWLASHMDVNAIDLTGAAGDEEHARELELAATDNLKRVLRPEPAEPDWTADPG
                     IARLTAFLETKTVWHPIGV"
     gene            3494..4291
                     /locus_tag="VH419_11575"
     CDS             3494..4291
                     /locus_tag="VH419_11575"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY7044298.1"
                     /translation="MAVERDPLAHWPDRTRLTDDTGRTLLVYSPGESTRGGRPLADGV
                     WRPDDVRLEDAVAASSQRLGGYAVSTPDPELAVSWQRAGADLLRHAHSMTHDLHQVAE
                     PDPVDVAIHPLTPAQVARHARRLGEINAAAYPPGHPDHHNDTLDAALAEMGAISRGEA
                     LGPMLPASRVATHAGAIVGAVFIVDRPGSAPEGGPWVLDVFRDPGSPLRGVGRALLLA
                     ALHSAKRSALPSMTLVVSHGNEGARRLYASVGFVDRDESWTLALPAQ"
     gene            complement(4346..4951)
                     /locus_tag="VH419_11580"
     CDS             complement(4346..4951)
                     /locus_tag="VH419_11580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044299.1"
                     /translation="MQRLTTLALSAAVAGTFGLIGAAGGSVMSANADTSSHPTIRGTD
                     ARSYAKVDSVTLPLRTSSPELDDCFPHARAKVKVDLTTDDIGKDTFRINARGLRPRTD
                     FTVFLIQKAGAPFGHVEYIGDFSTNRWGRGHNTFTLIAEEAFAFNNATGNRADLNKVG
                     FWFADPRADNGCLGAGSPVTGFDGDASAGVQMMNSGTHKLP"
BASE COUNT          818 a         1778 c         1822 g          824 t
ORIGIN      
        1 ggtgcggcga gggcaggtgg atgcatcgca aggcggagga gggaggcgat gcgcagcatc
       61 gtcgaccgac gacaacgccg cgaggcgccg cctggaccgg cgcaacggga aaaagacttc
      121 cgacgcggga cactagactg acgaacgtga ctgtttcccc cgcgacgtcc ctcgcgccgc
      181 ggtttctcga gtcgctcgag gatgcggtgt cctcgccggc cgcgttgcgg cggttcctgc
      241 acggactgcc gggcgtcgac caggtcgggg ccgaggcacg agccgccacg ctggcctcgc
      301 ggtccatcaa gaccaccgcg aaggcctggg cgctcgacat ggcgatctcg atgatcgacc
      361 tcaccacgct ggagggccag gacaccacgg ggaaggtgcg cgcgctgtgc gcgaaggcgc
      421 ggcagcccga cccggcggat ccgagctgtc cgcgcgtggc ggccatctgt gtctaccccg
      481 acatggtcgc aaccgccaag actgcgctcg gcagctccgg ggttcaggtc gcctcggtgg
      541 cgacggcgtt cccgtccggc cgggcgatgc tcgacgtgaa gctccaggac accagggacg
      601 cggtggcggc aggcgccgac gagatcgaca tggtgatcga ccgcggtgcg ttcctttccg
      661 gtgactacca gcaggtcttc gacgagatcg tcgcagtgaa gcaggcctgc gcccgcccag
      721 gcggcgggga gcatgctcac ctgaaggtca tcatggagac cggcgagctg gtgacgtacg
      781 acaacgtacg ccgggcatcg tggttgtcga tgctggccgg agccgacttc atcaagacgt
      841 ccaccggcaa ggttcagccg gccgcgacca tgccggtcac cctcgttatg ctcgaggcgg
      901 tccgcgactt ccgcgaggcc accggccgca tggtgggggt caagcccgcc ggcgggatca
      961 gcaccgcaaa ggacgcgatc aagtacctcg tgatggtcaa cgagacggcc ggcgaggact
     1021 ggttgacgcc gacgtggttc cgcttcggcg cgtcccgcct cctgaacgac ctgctgatgc
     1081 agcggaccaa gcttgcgacc ggtcggtact ccggtcccga ctacttcaca ctcgactagc
     1141 tccacgaggg gtgtcaccca gccatggcat tcgaatacgc gccggcaccg gagtcccggt
     1201 cgatcgttga cctcaagtcg tcgtacgggc tgttcgtcaa cggcgagttc gtcgacggcg
     1261 gcggtcacgc gttcaagtcg gtgagccctg cgacggagga agtgctcgcc gagatcgccg
     1321 aggcgaacga gacggacgtc gacctcgccg tcaaagctgc tcgtcgcgcc gccacgcgcg
     1381 tctggtcgag gatgtccggc gccgaacggg cgaagtacct ctatcggatc gctcggatcc
     1441 ttcaggagcg ctcccgcgag ctcgcggtgc tcgagtcgct cgacaacggc aagccgatcc
     1501 gtgagtcgcg ggatgtcgac gtcccgttgg tcgcggcgca cttcttctac tacgccggct
     1561 gggccgacaa gctcgagtat gccctgcccg gaaacaggca cagcggcccc gaccctcggc
     1621 cgctcggagt cgccggccag gtgatcccgt ggaacttccc gctgctcatg ctcgcctgga
     1681 agatcgcgcc ggctctcgca tgcggcaaca cggtcgtgct caagcccgcc gagacgacgt
     1741 cgctgacggc gttgctgttc gcggagatct gccagcaggc ggacctgccg ccaggcgtcg
     1801 tcaacatcgt cacgggggcg ggcgagaccg gccgcgcact cgtcgcccat cccgacgtcg
     1861 acaaggtcgc gttcaccggc tccaccgagg tcggcaagct gatcgcgcgt accgtggccg
     1921 gctccgacaa gaaggtcacg ctcgagctcg gtggcaaagc ggcgaacatc atcttcgacg
     1981 acgcgccggt cgaccaggcc gtcgagggca tcgtcaacgg gatcttcttc aaccagggcc
     2041 acgtgtgctg tgcgggttcg cggttgctcg tgcaggagag cgtgtacgac gaggtgctgg
     2101 ccaggctgaa gcgacggatg tcgacgctgc gcgtgggcga cccgctggac aagaacaccg
     2161 acgtcggcgc gatcaactcc tcggagcagc tggcgaagat ccgtgagctg tccgagatcg
     2221 gcgagaccga aggtgccgag cggtggtcgc cgaagtgcga cctgccggac cgtgggttct
     2281 ggttcgcacc gacgatcttc accggtgttt cgcaggcgca ccggatcgcc cgcgaggaga
     2341 tcttcggtcc ggtgctctcg atcctgacgt tccgcacgcc cgccgaggcg gtcgagaagg
     2401 ccaacaacac tccgttcggc ctgtcggcgg gtgtctggac ggagaagggc tcgaggatcc
     2461 tgtggacggc caaccagctg cgcgccggcg tggtctgggc caacacgttc aaccggttcg
     2521 acccgacgag cccgttcggc ggctacaagg agtccggctt cggccgggaa ggcggtcgcc
     2581 acggtttgga ggcctacctt gcccactgag cgcagtaccg cacggctgga cgtgcggaag
     2641 acctacaagc tgtacgtcgg cggcgcgttc ccgcgcagcg agtcaggccg ctcctacgtg
     2701 gtcaacgacg cgaagggtcg cttcctcgcg aacgccgcgt acgcctcgcg caaggatgcg
     2761 cgcgacgccg tcaccgcggc gcgcaaggcc ttccccggct ggtcgggccg gacgccgtac
     2821 aaccgcggcc aggtggtcta ccgggtggcg gaggtgctcg aggggcgacg cgaccagttc
     2881 gtcgacgagg tgcagcgcag cgagggtctg acgaagcgac aggcggccgg cgtcgtcgac
     2941 gcgacggtcg accggctcgt ctggtacgcc ggctgggccg acaaggtcgc ccaggtcgtc
     3001 ggtgggacca acccggtggc cggtccgtac ttcgacttct cgatcccgga gcccaccggt
     3061 gtggtggccg tgctcgcgcc gcaggagtcg tcgctgctcg gtctcgtcag tgtcctcgcg
     3121 ccggtcgtcg tcaccggcaa cacctgcgtg gtggccgcgt cgtacgagcg gccccttccg
     3181 gcgatcaccc tgtcggaggt gctggcgacg tccgacgtac ctggcggggt cgtgaacgtg
     3241 ctgacggggc ggctcgccga cacggcgccg tggctggcgt cgcacatgga cgtcaacgcg
     3301 atcgacctga ccggtgctgc cggtgacgag gagcatgcgc gggagctcga gctggcggcc
     3361 accgacaacc tcaagcgcgt gctgcgtccc gagccggccg aacccgactg gacagcagac
     3421 ccaggcattg cccggctgac ggcattcctg gagaccaaga ccgtctggca tccgattggc
     3481 gtctgacccg agcatggcgg tcgagcgcga cccgctcgcg cactggccgg accggacccg
     3541 gttgaccgac gacaccggtc gaacgctgct cgtctacagt cccggcgagt cgacccgtgg
     3601 cggcaggccg ttggccgacg gggtgtggcg gccggacgac gtacggctcg aggacgccgt
     3661 tgcggcatcg tcgcagcgcc ttggggggta cgccgtctcg acaccggatc ccgagctcgc
     3721 ggtctcatgg cagcgcgccg gtgccgacct gctcaggcac gcgcacagca tgacgcacga
     3781 tctgcaccag gtcgccgagc cggacccggt ggacgtcgcg atccatccgc tcacgcctgc
     3841 gcaggtggct cgacacgcac gacggctggg cgagatcaac gccgccgctt acccgccggg
     3901 ccatcccgac caccacaacg acacgctcga cgcggctctg gccgagatgg gcgcgatctc
     3961 gcgtggtgag gcgctgggcc cgatgctccc ggcctcccgc gtcgcgacac acgccggcgc
     4021 catcgtggga gcggtcttca tcgtcgaccg cccgggttca gcgccggagg gaggtccgtg
     4081 ggtactcgac gtcttccgag accccggttc accgctgcgt ggtgtcggcc gcgcgctgct
     4141 gctcgccgcg ctgcactcgg cgaagcggtc ggccctgccg tcgatgacgc tcgtggtcag
     4201 ccacggcaac gaaggcgccc gcaggctgta cgcgtcggtg gggttcgtcg accgggacga
     4261 gagctggacc ctcgccctgc ccgctcagtg agttgtcaga ggtgcatcga gccatcgccc
     4321 gatgcacctc tgacagttgg gtggctcagg gcagcttgtg cgtccccgag ttcatcatct
     4381 gtacgccggc tgacgcgtct ccgtcgaagc ctgtgaccgg gctacccgca ccgaggcagc
     4441 cgttgtccgc acgtgggtcg gcgaaccaga agccgacctt gttgaggtca gcccggttgc
     4501 cggtcgcgtt gttgaacgcg aacgcctcct cagcgatcag tgtgaacgtg ttgtgaccgc
     4561 gtccccaccg gttcgtgctg aagtcgccga tgtactccac gtgaccgaac ggcgcccctg
     4621 ccttctggat gaggaagacc gtgaaatctg tcctcggacg caggccgcgc gcgttgatcc
     4681 ggaacgtgtc cttcccgatg tcgtccgtgg tcagatccac cttgaccttc gcacgtgcgt
     4741 gcgggaagca gtcgtccagc tccggcgacg acgtcctgag cggcagcgtc acgctgtcga
     4801 ccttcgcgta tgagcgcgca tccgtgcctc ggatcgtcgg gtggctcgac gtgtcggcgt
     4861 tcgccgacat caccgagcca ccggccgcac cgatgaggcc gaacgtgccc gctaccgcag
     4921 cggacagcgc gagtgtggtg agtcgttgca tcattgtccc ccttgtgagc gtgcgcacga
     4981 cgtcgcgtgc gcaagccccg agcctggcgc cggaactgat cgtgggcatc ggggatatcc
     5041 cggacgtccg cgaagatcag ggagacggtg gtctacggcc agcggaccga cacaatgtgt
     5101 cgcgtgcctg aagttccccg tcgcgtcgtc ttgctggccg gtccgtccgg cgccgggaag
     5161 tcacgactcg cgtcacggct cggcctgccg atccttcagc tcgacgacta ctacaaggac
     5221 ggcgacgacc caagcctgcc gc
//
LOCUS       DATBFZ010000384         8729 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_6369700, whole genome shotgun sequence.
ACCESSION   DATBFZ010000384 DATBFZ010000000
VERSION     DATBFZ010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 8729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8729
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6369700"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2873
                     /locus_tag="VH419_11585"
     CDS             <1..2873
                     /locus_tag="VH419_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HEY7044300.1"
                     /translation="VVPKTVDAAKRAVAVCRRHRVPVVSRGGGTSLAGQGTNRAVVLD
                     FSKYCNRLVSVDAEAKTCVVEPGIVLDELNDQLADTGLRFGPEPATHMNCTLGGMIGN
                     NSCGATAQRTGKVVDNIAELDVLLYDGTRFTCGPTDDTTYREIERRGDRRAEIYRTLR
                     RIRDEHADQIQARYPDIPRRVSGYNLDSLLDEHGFDVAALLVGSEATLVTVLRATLKL
                     VPVVKERTLVVLGYRDIATAADAVPAVVEHDPIALEGVDSYLVHDEELKGLNPDAMAE
                     LPQGSAFLMVQFGGETVEEADERADAMLTAIGKTEHDPQVAFFDDPAHEHALWMVREA
                     GLGATAHVPAHPDTFEGWEDSAVPPEKLGDYLRDLHELFDEFGYLTDAGPSLYGHFGQ
                     GCVHTRIPFNLHAPRGVTDYRAFMERAADLVVSYGGSLSGEHGDGQSRGELLSKMFGT
                     EIVEAFGQVKTLFDPDNMMNPGKVVFPAKLDEHLRLGAHWAPSTPRNLFFHYPDDGGS
                     FVQAANRCVGVGKCRQDTHSAGQVMCPSYQVTKDEHHSTRGRARLLFEMLQGHPDSPI
                     TDGWRSTDVRDALDLCLACKGCKKDCPASVDMATYKAEFLAHHYAGRLRPRADYATGW
                     LPVVGRAVARSHLAGPVNVVSHTPGVRRLLTRAAGLEHRPIPVFARQTLQHWWQQREP
                     QPQRSRGTVLLWPDTFTNLFAPQIGRAAVEVLERAGWSVTMPTEPLCCGLTWISTGQL
                     KTATKMLRRTAQTLAPHVRAGGLVVGLEPSCTAVFRSDAHELFPDDQDIARLEDATVT
                     FAELLENHTPGWEPTRHRRPYRDAIAQVHCHQHAVLGWDADHHLVERIGVEAEPLESG
                     CCGLAGNFGFTAGHGQVSKALAERVLLPRVRAAGPETAVLADGFSCRTQIHDLDGGRT
                     AVHLAELVAEMTRPPCAAALPKPRRRHQRRTPFTTRTTDARS"
     gene            2876..4666
                     /locus_tag="VH419_11590"
     CDS             2876..4666
                     /locus_tag="VH419_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017936303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-requiring protein"
                     /protein_id="HEY7044301.1"
                     /translation="MSTTVADYLLERLRDWDVTTVFAYPGDGINGILAAFGQADNKPM
                     FVQSRHEEMSAFEAVGYAKFTGQVGVCMATSGPGAIHLLNGLYDAKLDHVPVVAIVGQ
                     TNRSAMGGAYQQEVDLLTLFKDVASDYVQTVTVPEQLPNVLDRAMRTAQARQGPTALI
                     IHADVQELDYSPPGHAFKMVPSSRGVSWGRTAPNDAAVQHAADILNAGAKVAILAGSG
                     ARGAREELMQVADLLGAGVAKALLGKDVLSDELPYVTGSIGLLGTRPSYQMMSDCDTL
                     LTVGSSFPYTQFMPGLDQARGVQIDIDPSLIGMRYPYEVNLVGDAAATLAALIPLLEK
                     KDDRSWRDTIEHNVAQWWETMAAEAMVEADPLNPMRIFSEASPRLPADSIVVADSGSA
                     ANWYARQLRFRGDMRGSLSGTLATMGPGVPYAIGAKFGHPDRPVVVFEGDGAMQMNGL
                     AELITIKHYWQQWSDPRLVVAVLHNNDLNQVTWEMRAMQGAPKFVESQRLPDVSYADF
                     AAGLGLAARTITDPDQLGDAWEQALAADRPTVLDVHCDPDVPPIPPHSTFQQVKDTTK
                     ALLTGDADRWGVVKEGVKTKAQELLPGRDD"
     gene            4670..5803
                     /locus_tag="VH419_11595"
     CDS             4670..5803
                     /locus_tag="VH419_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009077218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEY7044302.1"
                     /translation="MAKLVEAVEAVGARSYTIDTDSPEADGTFDWTSTTIVIATVEAA
                     GAVGLGYTYGPQACASVVTDMLRPVVLGTDPMDTPGTHLSMRKAVRNAGRPGVAGGAI
                     SAVDIALWDLKSRLLDLPLHRLLGTVRRDVPVYGSGGFTTYDDRQLHDQLTGWVVEQK
                     IGMVKIKIGESSGTDVPRDVRRMRAARESIGSDVELFVDANGAYTAKQAIRLMDTVAD
                     TDVRWFEEPVSSDDLAGLKRVRDVVRADVTAGEYGYDLAYFQRMLAADAVDCVQVDVT
                     RCGGITEWLRIAALADAHGLEVSGHCAPHVHAAVAAATQNLRHLEWFHDHVRIENQLF
                     EGTLDPTGGTITPSDERTGHGLTLEHDVVPAGMPAESDGLGHG"
     gene            complement(6270..6641)
                     /locus_tag="VH419_11600"
     CDS             complement(6270..6641)
                     /locus_tag="VH419_11600"
                     /inference="COORDINATES: protein motif:HMM:NF015953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEY7044303.1"
                     /translation="MFKALLISWLVLAVAIAITAAIVPSVDIEGGFFALLGVALIFGL
                     VNALIGPVIRLISLPLTVITFGIFALVINGILLAITAGLSSSLDVGGFFATILAALLI
                     SIFATIIGLAVNFLFDRREPA"
     gene            complement(6656..>8729)
                     /locus_tag="VH419_11605"
     CDS             complement(6656..>8729)
                     /locus_tag="VH419_11605"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEY7044304.1"
                     /translation="MTEEASPPIRRRLGRRFQPGDLIRVVVAWFVAAVSLALTAAILP
                     GLSGDGLWSWALVAIVAVVVGAVVRPVLVDVSARIGWIAVFLLAVAGQALVLTIAIEL
                     VPSVHSSSFWTSIAASFIAAAITTLLTWWATSGTDEAFTASLVRRGNRMGRSVADPDV
                     EGVVFVQLDGVSFPVLSWAVQAGLLPTVSRWLHDGSHRIHEWRAQIPCTTPASQIGIL
                     HGDVSGVPAFRWYDRELGRVLVANRPADAAIIEGRASDGRGLLADDGVSVSNVFSGDA
                     VRSAMTMSRMSASRGSRETRRAVAWYLARPDGFARSLTRTVVEVARERYQAAQQRRRD
                     VRPRVHRGWTFAMLRAVSNGVLRDLTTAVVADEVLRGTKVVYVDYVDYDEIAHHAGVF
                     RAESLAALEAVDAVLASLEQIVAVARRPYHLVLVSDHGQSQGTIFADAHGIDLAGLVS
                     SLVDQAVSAVQEPVESWGHAEAVLGDMGPDGRTGRMVDSATRRVDRKVAAEPTPAESL
                     IVLGSGNLGLVYAPEPTRLSREDIDIRWPALIDGLTSHPGIGFVACLSQGRPLAIGGG
                     GHRWLDDGDVVGDDPLAPYGVDAAERLCRTVMLDLAPDLYVNSSVDETTLEVGAFEPL
                     VGSHGGLGGWQDRGMLVVPTMLDDFVRPVDSAAGLHRIFVAMLEHLGHRTNLTSGSSS
                     KDLSSSGG"
BASE COUNT         1493 a         3048 c         2898 g         1290 t
ORIGIN      
        1 tggtggtgcc gaagaccgtc gacgcggcga agcgggcggt tgcggtctgt cgccgccacc
       61 gggtccctgt cgtgtcacgc ggcggcggaa catccttggc cggacagggg accaaccgcg
      121 cggtcgtgct cgacttctcc aagtactgca accggctcgt ctccgtcgat gccgaagcga
      181 agacctgtgt cgtcgagcca gggatcgtgc tcgacgagct caacgaccag ctcgccgaca
      241 ccggactgcg attcggcccc gagcctgcaa cacacatgaa ctgcaccttg ggcgggatga
      301 tcggcaacaa ctcctgcggc gccaccgcgc agcgcaccgg caaggtcgtg gacaacatcg
      361 ctgagctgga tgtgctgctc tacgacggga cgcgcttcac ctgtggaccg accgacgaca
      421 cgacataccg cgagatcgag aggcgcggtg accgcagggc ggagatctac cgaacgctac
      481 ggcggatccg agacgagcac gccgaccaga tccaggcccg ttacccggac attccccggc
      541 gggtctccgg ctacaacctc gactcgttgc tggacgaaca cggcttcgac gtcgccgccc
      601 tcctggtggg cagcgaggcg accctggtga ccgtgttgcg ggccaccttg aagctggtac
      661 cggtcgtgaa agaacgcacg ctggtcgtgc tcggctaccg cgacatcgcc accgccgccg
      721 acgcggtgcc ggctgtcgtc gagcacgacc cgatcgcgct cgaaggggtc gacagctacc
      781 tggtccacga cgaggagctc aagggcctga atcctgatgc catggccgag ctgccacagg
      841 gctccgcctt cctgatggtg cagttcggcg gcgagacggt cgaggaagcc gacgagcgag
      901 ccgacgccat gctgacggcc atcggcaaga ccgaacacga cccacaggtg gcgttcttcg
      961 acgaccccgc gcacgagcac gcgctgtgga tggtccgcga agccggcctc ggagcaacgg
     1021 cgcacgtccc ggcgcacccg gacacgttcg agggctggga ggactcggcg gtgccaccgg
     1081 agaagctcgg cgactacctt cgcgacctgc acgaactgtt cgacgagttc ggctatctca
     1141 ccgacgcggg gccgtccttg tacgggcact tcgggcaagg ctgtgtccac acccgcatcc
     1201 cgttcaacct gcatgcgcca cgtggcgtga cggactaccg cgccttcatg gagcgcgcgg
     1261 cagacctcgt ggtgtcctac ggcggttcgc tgtccggtga acacggcgac ggccagtccc
     1321 gtggcgagct gctctcgaag atgttcggga ccgagatcgt cgaggcgttc ggccaggtga
     1381 agacgttgtt cgacccggac aacatgatga accccggcaa ggtggtgttc cccgccaagc
     1441 tcgacgagca cctccggctg ggtgcgcact gggcaccgtc gaccccgcgc aacctgttct
     1501 tccactaccc ggacgacggc ggctcgttcg tccaggccgc gaaccggtgc gttggcgtcg
     1561 gcaagtgccg gcaggacaca cactccgccg gccaggtgat gtgcccgtcg taccaagtca
     1621 ccaaggacga gcaccactcc acacgtggcc gcgcacgcct gctgttcgaa atgctgcaag
     1681 gtcatcccga ctcgccgatc accgacgggt ggcgctccac ggacgtccgc gacgcgctcg
     1741 acctgtgtct ggcctgcaag ggctgcaaga aggactgccc ggcctctgtg gacatggcga
     1801 cgtacaaggc ggagttcctc gcccaccact acgccggccg gctccgccct cgcgcggact
     1861 acgccaccgg ctggctgccg gtcgtcggcc gggcggtcgc ccgcagccat ctcgccggtc
     1921 cggtgaacgt cgtcagccac acgccgggtg tccgtcgcct cctgacgagg gcggccgggc
     1981 tcgagcaccg cccgatcccg gtcttcgcgc gccagacact ccagcactgg tggcagcagc
     2041 gtgagccgca gccccagcgc agccgcggca cggtcctgct gtggccggac acgttcacca
     2101 acctcttcgc accccagatc ggcagagctg cggtcgaggt gctggagcgt gcgggctggt
     2161 cggtgacgat gccgaccgaa ccgctctgct gcgggctgac ctggatctcg accgggcagc
     2221 tcaagacggc gaccaagatg ctgcgacgga cggcacagac ccttgcgcct cacgtgcgcg
     2281 caggcgggct ggtcgtcggt ctcgaaccca gctgcaccgc ggtgttccgc tccgatgcgc
     2341 acgaactgtt ccccgacgac caggacatcg cgcggctcga ggacgccacc gtcacgttcg
     2401 ccgagctgct ggagaaccac acaccgggat gggagccgac ccggcatcga cgcccgtacc
     2461 gcgacgccat cgcccaggtg cactgccacc aacacgccgt tctgggctgg gatgccgacc
     2521 accacctggt cgaacgcatc ggggtcgagg cggagccgct cgagtcgggc tgctgcggcc
     2581 tcgcggggaa cttcggcttc accgccggac acggtcaggt cagcaaggca ctggccgaac
     2641 gcgtgctcct cccgcgggtg cgcgctgccg gacctgagac agcggtgctc gccgacgggt
     2701 tctcctgccg aacgcagatc cacgacctgg acggcggccg cacagccgtg cacctcgccg
     2761 agctcgtggc agaaatgact cggccgccct gcgcagcggc gctcccgaaa ccacgtcgcc
     2821 ggcaccaacg gcgtactccg ttcacgacac gcaccaccga tgcaaggagc tgaacatgtc
     2881 aacgaccgtc gccgactact tgctggagcg tcttcgcgat tgggacgtca ccacggtctt
     2941 cgcctacccc ggtgacggga tcaacgggat cctggcggcg ttcggccagg cggacaacaa
     3001 gccgatgttc gtgcagtcgc gacacgagga gatgagcgcg ttcgaggctg tcggctacgc
     3061 gaagttcacc ggccaggtcg gtgtgtgcat ggccacgtcc ggccccggag cgatccacct
     3121 gctcaacggc ctgtacgacg ccaagctcga ccacgtcccc gtggtggcga tcgtcggtca
     3181 gacgaaccgg tcggcgatgg gcggcgccta ccagcaggag gtcgacctcc tcacgctgtt
     3241 caaggacgtg gcgtcggact acgtccagac cgtcaccgtc ccggagcagc tgcccaacgt
     3301 tctggaccgc gccatgcgga cggcgcaggc caggcagggt ccgaccgcgc tgatcatcca
     3361 cgccgatgtg caagagctcg actactcgcc acccggacat gcgttcaaga tggtcccttc
     3421 gagccgcgga gtctcctggg gtcggacggc gccgaacgac gcggcggtcc agcacgctgc
     3481 ggacattctc aacgccggcg cgaaggtggc gatcctggcc ggttcgggcg cgcgaggggc
     3541 ccgcgaggag ctgatgcagg tcgctgacct gctcggcgcc ggtgtcgcca aggccttgct
     3601 cgggaaggac gtgctgtccg acgagctgcc ctacgtgacg gggtcgatcg ggctgctcgg
     3661 tacgcggccg agctaccaga tgatgagtga ctgcgacacc ttgctgaccg tcgggtccag
     3721 cttcccctac acacagttca tgcccggcct cgaccaggcc cgcggggtcc agatcgacat
     3781 cgatccgagc ctgatcggga tgcggtatcc ctacgaggtc aatctcgtgg gcgacgccgc
     3841 cgccacgctc gctgccctga tcccgcttct ggagaagaag gacgaccggt cctggcggga
     3901 caccatcgag cacaacgtgg cgcaatggtg ggagacgatg gcggctgagg cgatggtgga
     3961 ggccgatccg ctcaatccga tgcggatctt ctccgaagcg tcgccacgtc ttcccgccga
     4021 ctcgatcgtt gtcgccgact ccggatcggc tgccaactgg tatgcgcggc agctgaggtt
     4081 ccgcggcgac atgcgcgggt cactgtccgg caccctggcg acgatggggc ccggtgtccc
     4141 gtacgccatc ggcgcgaagt tcggtcaccc ggacaggcca gtcgtcgtgt tcgagggcga
     4201 cggtgccatg cagatgaacg gtctggccga gctcatcacg atcaagcatt actggcagca
     4261 gtggtccgat ccgcgcctgg tggtcgccgt gctccacaac aacgacctga accaggtgac
     4321 gtgggagatg cgggcgatgc agggcgcccc gaagttcgtc gagtcccaac ggctcccgga
     4381 tgtctcgtac gccgacttcg ccgccggcct tggtctcgcc gctcgcacca tcaccgaccc
     4441 ggaccagctg ggtgacgcct gggaacaggc actcgctgcg gatcggccga ccgtcctcga
     4501 cgtgcactgc gacccggacg tcccaccgat cccgccacac tcgaccttcc agcaggtgaa
     4561 ggacaccacg aaggcgctgc tgaccggcga cgcggaccgc tggggtgtcg tcaaggaagg
     4621 cgtcaagacg aaggcgcagg agctcctccc cggtcgcgac gactgagtca tggcgaagct
     4681 ggtggaggcg gtcgaggcgg tcggggcacg gtcgtacacg atcgacaccg actcgcctga
     4741 ggcggacggc acgttcgact ggacgtcgac gacgatcgtg atcgccacgg tcgaggccgc
     4801 cggggccgtc ggcctcgggt acacctacgg cccgcaagcc tgcgcttccg tcgtcaccga
     4861 catgctgcgg cccgtcgtgc tgggtaccga tccgatggac acgcccggaa cgcacctgtc
     4921 catgcggaag gcggtgcgca atgccggacg gcccggcgtc gccggtggcg cgatctccgc
     4981 ggtcgacatc gccttgtggg acctgaagag ccgactcctc gaccttccac tccatcgact
     5041 gctcgggacc gttcgtcgag acgtaccggt gtacggcagc ggcgggttca cgacatacga
     5101 cgacaggcag ttgcacgacc agctcaccgg ctgggtggtc gagcagaaga tcggcatggt
     5161 caagatcaag atcggcgagt cctcggggac cgacgtccct cgggacgtac gtcgtatgcg
     5221 cgccgcccga gagtccatcg gttccgacgt cgagctgttc gtggacgcga acggggcgta
     5281 caccgcgaag caggcgatcc gactgatgga caccgttgcc gacaccgacg ttcgatggtt
     5341 cgaggaaccc gtctcgtcgg acgacctggc cggcctcaag cgggttcgcg atgtcgtccg
     5401 cgccgacgtc accgccggcg agtacggcta cgacctggcc tacttccagc ggatgctggc
     5461 cgccgatgcg gtcgactgcg tccaggtcga cgtcacccga tgcggcggca tcacggagtg
     5521 gctgcgcatc gctgcgctgg ccgacgctca cggcctggag gtgtccggcc actgcgcgcc
     5581 acacgttcat gctgccgtgg ccgccgcgac gcagaacctg cgacacctcg agtggttcca
     5641 cgaccacgtc cggatcgaga accagctgtt cgagggcacg cttgacccga ctggcggcac
     5701 gatcactccg tcggacgagc ggaccggcca cggtctgacg ctggagcacg acgttgtccc
     5761 cgccggcatg ccggcggaat ccgacggact tggccatggc tgagcccgag cagttcgatc
     5821 cgatcctgcc gcaacggttg ccgtaccgac cccggtggat ctgggcaggc gtcgcgctcg
     5881 ttctggccgg cctcggcgcc ggcgtggtag cgatcatcgc gttcagctgg gtgatcgcgg
     5941 gagtcgccgt gctggtgctg ctgggaggag tgggtgcggc ctggcacggg cgcatcatgc
     6001 gggacacgca tggcaacagc ggcccgctcg ccatggaatg ggcgaacctg aaggagcctc
     6061 ggcagcacga caccaccggc ttcgacccga agcagacagg tcacgtcata cccgacggac
     6121 accgcgcggc gcgccaccag ccggccaagg tgacagatgc tgaccgcgcc cagctgtcgg
     6181 gtttgctccg ggcccccggc aattgcggga atctgcacgt ccgaagaagt gtcttggggg
     6241 tacgtttccc gcaattcgcg gtaagccgat caggcgggct cgcggcggtc gaagaggaag
     6301 ttcacggcca agccgatgat ggtcgcgaag atcgagatca gcagcgccgc gaggatggtc
     6361 gcgaagaagc cccccacgtc cagcgagctg ctgagcccgg ccgtgatggc gagcaggatc
     6421 ccgttgatca ccaacgcgaa gatgccgaac gtgatcaccg tgagcgggag tgagatcaac
     6481 cggatcaccg gtccgatgag tgcattcacg agcccgaaga tcagcgccac cccgagcagg
     6541 gcgaagaagc cgccctcgat gtcgacggac gggacgatcg cggccgtgat cgcgatggcc
     6601 acggccagca ccagccagct gatgagcagt gccttgaaca tgtcggactc ctatctcagc
     6661 cgcctgacga cgagaggtcc ttgctcgacg acccgctcgt caggttcgtg cgatgtccaa
     6721 gatgttcgag catcgcgacg aagatcctgt gcagacccgc cgcggagtcg acaggtcgca
     6781 cgaagtcgtc cagcatcgtc ggcacgacga gcatgccgcg gtcctgccag ccaccgagtc
     6841 ctccgtggct cccgacgagc ggctcgaacg ccccgacctc gagcgtcgtc tcgtccaccg
     6901 agctgttgac gtagaggtcc ggcgcgaggt cgagcatcac ggtccggcac aagcgttccg
     6961 cggcatcgac gccgtacggc gccagcgggt cgtcgccgac cacgtcaccg tcatcgagcc
     7021 accggtggcc acccccgccg atcgccaggg gacgtccttg tgagagacag gccacgaagc
     7081 cgatccccgg gtggctcgtc agcccgtcga tgagcgccgg ccagcggatg tcgatgtcct
     7141 cacgactcag ccgggtcggc tcgggcgcgt acaccaaccc gaggttcccc gagccgagga
     7201 cgatgagcga ctccgcgggc gtcggttcgg ccgccacctt cctgtcgacc cgacgcgtcg
     7261 ccgagtccac catccggccg gtcctgccgt ccgggcccat gtcccccaac acggcttcgg
     7321 cgtggcccca gctctcgacc ggttcctgca ccgcactgac cgcctggtcg accagtgacg
     7381 agacaaggcc cgcgaggtcg attccgtgtg catcggcgaa gatcgtcccc tgcgactgtc
     7441 cgtggtccga gacgagcacc aggtggtacg gacgtcgcgc caccgcgacg atctgctcga
     7501 ggctggccag caccgcgtcg accgcctcca acgccgccag tgactcggcc cggaagacgc
     7561 ccgcgtggtg ggcaatctcg tcgtagtcga cgtagtcgac gtagacgacc ttcgtcccac
     7621 gcagtacctc gtcggccacg acggccgtcg tgaggtctcg caggacaccg ttgctgacgg
     7681 cgcgcagcat ggcgaacgtc caaccccggt gcaccctcgg gcgcacgtcc cgccgccgct
     7741 gctgggccgc ctggtaccgc tcgcgtgcca cctcaacgac cgtgcgcgtg aggctgcgcg
     7801 cgaagccgtc cggtcgggcg agataccacg cgacggcacg tcgggtctcc ctcgagccac
     7861 gacttgcgga catccggctc atcgtcatgg ccgagcgcac cgcgtcgcca ctgaacacgt
     7921 tgctcacgga cacgccgtcg tccgccagca gcccgcgccc gtcactcgcc cggccttcga
     7981 tgatggcagc gtctgcggga cggttggcca ccagcacccg ccccagctcg cggtcgtacc
     8041 aacggaaggc cggtactccg gagacgtcac cgtgcaggat gccgatctgg ctggccggcg
     8101 tggtgcacgg gatctgggca cgccattcgt gtatccggtg cgagccatcg tgcaaccagc
     8161 gactgacggt cggtagcaag ccggcctgga ctgcccagct caacaccggg aacgagactc
     8221 cgtcgagctg gacgaacacc acgccttcca catcggggtc agcgacggac ctgcccatcc
     8281 ggtttccccg ccgcacgagg gacgcggtga acgcttcgtc ggtaccggac gtcgcccacc
     8341 aggtgagcaa cgtcgtgatc gccgcagcga tgaacgatgc ggcgatcgac gtccagaacg
     8401 agctggagtg gacgctcggc acgagctcga tcgcaatcgt cagcacgagc gcctggccgg
     8461 ccacagccag caagaacacc gcgatccagc cgatccgggc cgacacgtcg accagcaccg
     8521 gtcgcacgac cgcgccgacc acgaccgcga cgatggccac cagcgcccag gaccagaggc
     8581 cgtccccgct gagccccgga aggatcgccg cggtcagcgc gagcgagacg gcggcgacga
     8641 accatgcaac aacgacccgg atgaggtcac ccggctggaa gcgacgtccc agacgacgcc
     8701 ggatgggtgg cgatgcttcc tcggtcatg
//
LOCUS       DATBFZ010000385         1999 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_922584, whole genome shotgun sequence.
ACCESSION   DATBFZ010000385 DATBFZ010000000
VERSION     DATBFZ010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 1999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1999
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_922584"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="VH419_11610"
     CDS             complement(<1..494)
                     /locus_tag="VH419_11610"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008359086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HEY7044305.1"
                     /translation="MMFPIKGIALGCDYNPEQWPRSVWREDVRLMQAAGVGFVTLGVF
                     SWALLEPRPGVFDFGWLDEVVGLLADGGIAIDMATATAAPPPWLTTAHPEILPVDHAG
                     HTLWPGSRQSWCPSSPVFRAAALRLVAATADRYGSHPAVALWHVSNELGCHNALCYCD
                     VSAEA"
     gene            complement(491..1399)
                     /locus_tag="VH419_11615"
     CDS             complement(491..1399)
                     /locus_tag="VH419_11615"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEY7044306.1"
                     /translation="MTTTTTTTTTTTTTPPRTGRAPGRRIVLHVFLWIVALSWLFPIA
                     WAVLNSFRDYAYTAIHGYVSFGGFTIQNYVNAWNQGDFGLHFWNSAVITVPAVLITLF
                     LASCMAFVLARFSFKFNLTLLAVFLAANLLPPQSLLVPVYRMFRAIPLPEWLSSSGSL
                     LNNQIGVVLINVAFQLGFCTFVLSNYMKTIPADIYESARVDGASVTRQFFQLTLPLCR
                     PPLAALATLQVTWIYNEFFWATVLLQSGDKFPITSSLNSLQGQFFTDYNLLSAGSVLA
                     MLPVLIIFFALQRQFVSGLTLGSTKG"
     gene            complement(1396..>1999)
                     /locus_tag="VH419_11620"
     CDS             complement(1396..>1999)
                     /locus_tag="VH419_11620"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEY7044307.1"
                     /translation="MFLAVLLDKEIRFSRFYQTALYLPVVLSLALVGFVWQLMYSRDQ
                     GLINGVLHTQVDWYGDPNYNLWAVLVATSWRHIGYVMLIYLAGLKAVDNTLREAAAID
                     GASEAQTFFRIIFPVMRPINIIVLVITVIEALRAFDIVWVINKGRNGLELIATLVTAN
                     VVGEASRIGFGSAMATIMLLISSVFVAIHVTITMREERRK"
BASE COUNT          371 a          644 c          691 g          293 t
ORIGIN      
        1 gcctccgccg agacgtcgca gtagcagagc gcgttgtggc agcccagttc gttcgagaca
       61 tgccacagcg cgacggcggg gtgcgacccg tagcggtcgg cggtcgccgc gaccagccgg
      121 agggccgccg cgcggaacac cggcgagctc gggcaccacg actgcctgct gcccggccag
      181 agcgtgtgcc cggcgtggtc gaccgggagg atctccgggt gcgcggtggt gagccacggc
      241 gggggcgcgg ccgtggccgt cgccatgtcg atcgcgatgc cgccgtcggc gagcagcccg
      301 acgacctcgt ccaaccagcc gaagtcgaac accccgggcc gcggctccag cagcgcccac
      361 gagaacacgc cgagcgtcac gaacccgacg cccgccgcct gcatgagccg cacgtcctcg
      421 cgccacaccg agcgcggcca ctgctccggg ttgtagtcgc agcccagcgc gatgcccttg
      481 atggggaaca tcatcccttc gtgctcccca gggtcaggcc ggacacgaac tggcgctgca
      541 acgcgaagaa gatgatcagt acgggcagca tcgcgagcac ggagcccgcc gagagcaggt
      601 tgtagtcggt gaagaactgg ccctgcaggc tgttgagcga gctcgtgatc gggaacttgt
      661 cgccgctctg cagcagcacg gtggcccaga agaactcgtt gtagatccag gtgacctgca
      721 gggtcgccag cgccgcgagc ggcgggcggc acagcggcaa ggtgagctgg aagaactgcc
      781 gggtgacgct cgcgccgtcc acccgcgcgg actcgtagat gtcggccggg atcgtcttca
      841 tgtagttgga cagcacgaac gtgcagaagc cgagctggaa cgcgacgttg atgagcacga
      901 cgccgatctg gttgttcagc aggctccccg agctggacag ccactcgggg agcgggatcg
      961 cgcggaacat ccggtagacg ggcaccagca acgactgcgg tggcagcagg ttggcggcga
     1021 ggaacaccgc gagcagcgtc aggttgaact tgaagctgaa gcgcgccagc acgaacgcca
     1081 tgcacgaggc caggaacagc gtgatgagca ccgcgggcac ggtgatcacc gcggagttcc
     1141 agaagtgcag cccgaagtcg ccctggttcc aggcgttcac gtagttctgg atcgtgaagc
     1201 cgccgaacga gacgtagccg tggatcgcgg tgtaggcgta gtcgcggaac gagttgagca
     1261 ccgcccacgc gatggggaag agccacgaga gcgcgacgat ccacaggaag acgtgcagca
     1321 cgatgcgcct gccgggggcg cgcccggtgc ggggtggtgt cgttgttgtt gttgttgttg
     1381 ttgtcgtcgt cgtcgtcact tgcgccgctc ctcgcgcatc gtgatcgtca catggatcgc
     1441 gacgaacacc gagctgatca acagcatgat cgtcgccatg gcggagccga aaccgatccg
     1501 gctcgcctcg cccacgacgt tcgccgtgac gagcgtggcg atgagctcca acccgttgcg
     1561 acccttgttg atcacccaga cgatgtcgaa cgcccgcagc gcctcgatca ccgtgatcac
     1621 caggacgatg atgttgatcg gccgcatcac cgggaagatg atccggaaga aggtctgcgc
     1681 ctcgctggcc ccgtcgatcg cggccgcctc gcgcagcgtg ttgtcgacgg ccttgagccc
     1741 ggccaggtag atcagcatca cgtagccgat gtgccgccag ctggtggcga ccagcacggc
     1801 ccacaggttg tagttcgggt cgccgtacca gtcgacctgc gtgtgcagca ccccgttgat
     1861 caggccctga tcccgggagt acatcagctg ccagacgaac ccgacgagcg cgagcgacag
     1921 cacgaccggc aggtagagcg ccgtctggta gaagcggctg aaccggatct ccttgtccag
     1981 cagcaccgcg aggaacatg
//
LOCUS       DATBFZ010000386         5003 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_2556607, whole genome shotgun sequence.
ACCESSION   DATBFZ010000386 DATBFZ010000000
VERSION     DATBFZ010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 5003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5003
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2556607"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1127)
                     /locus_tag="VH419_11625"
     CDS             complement(<1..1127)
                     /locus_tag="VH419_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020574534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HEY7044308.1"
                     /translation="MTRYVFDFHEGSARQKDLLGGKGANLAEMTNLGLPVPPGFTITT
                     DACRAYLETGSEPDELADEVGAHLAALEKNMGRTLGDVDDPLLVSVRSGAKFSMPGMM
                     ETVLNIGLNDESVQGLAKISGSTRFAWDSYRRLLSMFGATVLDVPRSRFDDALDDLKE
                     DRGVKTDIELEAADFEALVATYKQVIKDATGHEFPQSPREQLDQAVRAVFSSWNTERA
                     KLYRRQERIPHDLGTAVNVVAMVFGNLGDDSGTGVCFTRDPATGQQGVYGDYLPNAQG
                     EDVVAGIRNTLPLSELEDRDKKSYDELLQIMATLERHYKDMCDIEFTVERGKLWMLQT
                     RVGKRTAAAAFRIAVQFVDEGLIDKDEALRRVTGDQLANLM"
     gene            1307..1801
                     /locus_tag="VH419_11630"
     CDS             1307..1801
                     /locus_tag="VH419_11630"
                     /inference="COORDINATES: protein motif:HMM:NF021193.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PGPGW domain-containing protein"
                     /protein_id="HEY7044309.1"
                     /translation="MALRKAWVTFAGWLLVVVGIIAMPLPGPGLLILLSGLVVLSQEY
                     EWAERRVEPVKEKAIQGAKQGVATYPRIAFAALSATAVVAVGVWWSYDPRIPTVWLIG
                     PRIPFHLGGWPTGSSIVLSGLIAWGVLIYSIKRYRKEALDERNSERRSSDDDRDASSS
                     PSPR"
     gene            1891..3288
                     /gene="lpdA"
                     /locus_tag="VH419_11635"
     CDS             1891..3288
                     /gene="lpdA"
                     /locus_tag="VH419_11635"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002536884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEY7044310.1"
                     /translation="MSSHFDVVVLGAGPGGYVAAIRAAQLGKKTAVVERKYWGGVCLN
                     VGCIPSKALLRNAELAYIFTREAKTFGISGDASFDFGAAFDRSRKVADGRVKGVHFLM
                     KKNAITEYDGWGTFTDPNTLSVELSDGGEETVTFDNAILATGATTRLLPGTELGDRVV
                     TYEEQIMTPELPASMIIAGAGAIGVEFAYVLTNYGVDVTIVEFLDRMVPLEDEEVSAE
                     LAKRYKKLGVKVLTSTRVDAIEDTGSGPVKVTVTKGDEQQVLEADKVLQAIGFAPRVD
                     GYGLDKAGVELTDRGAVAIDDRMRTNVSHIYAIGDVTAKLMLAHLAEAMGVAAAETIA
                     GAETMSIDYAMVPRATYCQPQIASFGYTEAQAKERGHDLKVAKFPFTANGKAHGLGDP
                     GGFVKIVADAEHGEFLGAHLIGPDVTELLPELTLAQQWDLTAAEVARNIHAHPTLTEA
                     IKEAVHGIAGHMINF"
     gene            complement(3483..4073)
                     /locus_tag="VH419_11640"
     CDS             complement(3483..4073)
                     /locus_tag="VH419_11640"
                     /inference="COORDINATES: protein motif:HMM:TIGR01926.1"
                     /note="This protein belongs to a clade of uncharacterized
                     proteins related to peroxidases such as the
                     alkylhydroperoxidase AhpD; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase-related enzyme"
                     /protein_id="HEY7044311.1"
                     /translation="MFITTVDEAAAEGEVADYYAQQRGAWGFLPNYAAAFSTRPDIAQ
                     AWNVLNQAVRQGMDRRRFEIATIAAARALRSTYCTAAHSKFLRDVCGDEPTLNSIVEQ
                     PSGATLDLQDRAVYEFAAKVAEDASSVEQADVDELRAVGLSDADIADVVFAASARSFF
                     TKVLDGLGAQLDVQTAQTFAPAVLESMIVGRPIAEG"
     gene            4272..>5003
                     /locus_tag="VH419_11645"
     CDS             4272..>5003
                     /locus_tag="VH419_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012918056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY7044312.1"
                     /translation="MDALTIRVFDLPDHLAAKADPALIAGDEAHLAAVGRSLEQSIAD
                     LSDRIDTARRSAGGTGQEAMDRDLEIHRLNARLRTLRRYGLDLCLGHIVTADEPEPMY
                     VGRLGLTDSAGRRLLHDWRSPAAEPFFGATHANPMGLTSRRRYRWTRGRISDYWDEVF
                     TADEFDRHSAALDDQSAFIASLGRSRSPQMPDVLGTIQADQDAIIRASSRGALVVDGG
                     PGTGKTVVALHRTAYLLYSDPRLGHE"
BASE COUNT          831 a         1707 c         1636 g          829 t
ORIGIN      
        1 aacatgaggt tcgccagctg gtcgccagtc acccgacgca gcgcttcgtc cttgtcgatc
       61 agaccttcgt cgacgaactg caccgcaatc cggaacgctg ccgcggcggt gcgctttccg
      121 acccgggtct ggagcatcca gagcttgccg cgctccacgg tgaactcgat gtcgcacatg
      181 tccttgtagt gacgctcgag ggtcgccatg atctggagca gctcgtcgta ggacttcttg
      241 tcgcgatcct ccagctccga cagcggaagg gtgttgcgga tgccggcgac gacgtcctcg
      301 ccctgggcgt tcgggaggta gtcgccgtag acgccctgct gcccggtcgc cgggtcgcgg
      361 gtgaagcaga cgccggtgcc ggagtcgtcg ccgaggttgc cgaacaccat ggcgacgacg
      421 ttgaccgcgg tgccgaggtc gtggggaatc cgctcttggc ggcggtagag cttggctcgc
      481 tcggtgttcc aggacgagaa gacggcgcgc accgcctggt cgagctgctc gcgcggtgac
      541 tgcgggaact cgtggccggt cgcgtccttg atcacctgct tgtacgtcgc gaccagcgcc
      601 tcgaagtcgg ccgcctcgag ctcgatgtcg gtcttgacgc cgcggtcctc cttgaggtcg
      661 tcgagcgcgt cgtcgaatcg tgagcgggga acgtcgagca cggtcgcccc gaacatcgag
      721 agcagccggc ggtaggagtc ccaggcgaac ctggtgctgc cggagatctt ggcgagcccc
      781 tggaccgact cgtcgttgag gccgatgttg aggaccgtct ccatcatccc cggcatcgag
      841 aacttcgccc ccgaccgcac ggagacgagc agcggatcgt cgacatcgcc cagcgtgcgg
      901 cccatgttct tctccagcgc cgccaggtgt gctcccacct cgtcagccag ttcgtccggc
      961 tcactgcccg tctcgaggta ggcgcggcag gcgtcggtcg tgatcgtgaa cccgggcggc
     1021 accggaagtc caaggttcgt catctcggcg agatttgcgc ccttgcctcc cagcaggtcc
     1081 ttctgtcggg cactgccctc gtggaagtcg aacacgtaac gggtcatcac acgtccttcg
     1141 cggctgagtc gacctggctg ccgaccggat ggtgggcacg gctggattgt cctcatcttg
     1201 ccgcgatcgg cgcgccttcg gaacagcgca cggtccggca accaggcgcc ggtaccgccg
     1261 tggtgtcgta ggctccctcc agaagacgac gcgacgaggg ggacgggtgg cactgcgcaa
     1321 ggcgtgggtg acgttcgcgg gctggctgct cgtggtcgtc ggcatcatcg ccatgccgct
     1381 gcccggcccg ggtctgttga tcctcctgtc cggactcgtc gtgctgtctc aggagtacga
     1441 gtgggccgag cgccgggtcg aaccggtgaa ggagaaggcc atccagggcg cgaaacaagg
     1501 cgtcgccacc tacccccgga tcgcctttgc ggcgctgtcc gcgaccgcgg tcgtggccgt
     1561 cggcgtctgg tggagctacg acccgcggat cccgaccgta tggctgatcg gtccccggat
     1621 ccccttccac ctcggcgggt ggccgaccgg gtcgtccatc gtcttgtcag ggctgatcgc
     1681 ctggggtgtg ctgatctaca gcatcaagcg ctatcgcaag gaagccctgg acgaacgcaa
     1741 cagcgaacgt cgctcctccg acgacgaccg cgacgcgtcc tcctccccct ctccccgttg
     1801 acccgcgcga tgttcgcggc actctgacgc tgccgcctct ggcggatgca cgcgcagccg
     1861 cctcccggct gagccaataa ggtgacttcc atgagctctc acttcgacgt ggtcgtgctc
     1921 ggtgccggac ccggcgggta cgtcgccgcc atccgcgccg cccaactggg caagaagacc
     1981 gcggtggtcg agcggaagta ctggggcggc gtctgtctga acgtcgggtg cattccgagc
     2041 aaggccttgc tgcgcaacgc cgagctggcc tacatcttca ccagggaggc caagacattc
     2101 ggcatctccg gtgatgcgtc gttcgacttc ggcgccgcgt tcgaccgcag ccggaaggtg
     2161 gccgacgggc gggtcaaggg cgtccacttc ctgatgaaga agaacgccat caccgagtac
     2221 gacggctggg gcaccttcac cgatcccaac acgctcagcg tcgagctctc cgacggtggc
     2281 gaggagacgg tgaccttcga caacgccatc ctggcaaccg gcgcgaccac gcgactgctc
     2341 ccggggaccg agctcggcga ccgggtggtc acctacgaag agcagatcat gacgccggag
     2401 cttccagcgt cgatgatcat cgccggtgcc ggcgcgatcg gggtcgaatt cgcgtacgtc
     2461 ctcacgaact acggtgtcga cgtcaccatc gtggagttcc tcgaccggat ggtcccgctt
     2521 gaagacgagg aggtctcggc cgagctcgcc aagcgataca agaagctcgg cgtcaaggtg
     2581 ctcaccagca cccgggtcga cgcgatcgag gacaccggct ccgggcctgt caaggtgacg
     2641 gtcaccaaag gcgatgagca gcaggtgctg gaggccgaca aggtcctcca ggcgatcggc
     2701 ttcgcacctc gcgtcgatgg ctacgggctg gacaaggccg gagtcgagct gaccgatcga
     2761 ggtgcggtcg ccatcgacga ccggatgcgc acgaacgtct cgcacatcta cgccatcggc
     2821 gacgtgacgg cgaagctcat gctggcgcac ctggcggagg cgatgggcgt cgccgccgcc
     2881 gagacgattg ccggtgccga gacgatgtcg atcgactacg cgatggtgcc ccgggcgacg
     2941 tactgccaac cgcagatcgc cagcttcggc tacaccgagg cccaggcgaa ggagcgcggc
     3001 cacgacctca aggtcgcgaa gttcccgttc accgccaacg gcaaggcaca cggactcggt
     3061 gacccaggcg gtttcgtgaa gatcgtggcc gacgccgagc acggcgagtt cctcggcgcc
     3121 cacctgatcg ggccggacgt caccgagctg ctacccgagc tcacgctggc ccagcagtgg
     3181 gacctcacgg cggccgaagt cgcgcgcaac atccatgccc acccgaccct gaccgaggcc
     3241 atcaaggaag ccgtgcacgg catcgcaggc cacatgatca acttctaggg ctcaaccgag
     3301 ttgtcagaac tccatggggc tatggctcaa cgtggttctg acaactcaac cgaccgcctt
     3361 cgcttctcgg acgtcgacaa ggcagccatg gttccgacct gccccgtccc tcgccgaggt
     3421 ggcaggtttc gttgctttct gtcggcgtgt cggccggcca gtgccacctc ggtcagtggc
     3481 gatcagcctt cggcgatggg acgcccgacg atcatcgact cgagcaccgc cggcgcgaac
     3541 gtctgcgccg tctgcacgtc cagctgtgcg cccaggccgt ccagcacctt cgtgaagaac
     3601 gagcgagcgg acgccgcgaa gaccacgtcg gcgatgtcgg cgtcggacaa accgactgcc
     3661 cgcagctcgt ccacatcagc ctgctcgacg ctcgacgcat cctcagccac cttggctgcg
     3721 aactcgtaga cggcgcggtc ctgcagatcc agcgtggcac cgctcggttg ctcgacgatc
     3781 gagttcagcg tcggttcgtc accgcacacg tcgcgcagga acttcgagtg cgcggcggtg
     3841 cagtacgtcg agcgcagcgc acgggcggcg gcgatggtcg cgatctcgaa ccgccgtcgg
     3901 tccatgccct gccggacggc ctggttgaga acgttccacg cctgcgcgat gtccggacgc
     3961 gtggagaatg cggcggcata gttgggcagg aagccccagg cgccacgttg ctgtgcgtag
     4021 tagtcggcga cttcgccttc ggcggccgcc tcatccacgg tggtgatgaa cacccgacca
     4081 tcttcgccgg ttgccctcca gaaacgcagc actttgcggt cacacacgga tggcgaggcg
     4141 tacacagaga gatggaaggg cagctacgcc cttccatttt caacgtataa cgcaccaggg
     4201 ggcttgccgc aagaccccgg tcaggtcgca caatcactag ccgtagcccg caccatggac
     4261 ggagtgtgac cttggacgcc ttgaccatca gggttttcga ccttcccgac cacctcgccg
     4321 ccaaggccga cccggcactg atcgccggcg acgaagcaca cctcgcagcc gtcgggagat
     4381 cgctcgagca gtcgatcgcc gacctgtcgg accgtatcga caccgctcgc cgatcggcgg
     4441 gcggcaccgg ccaggaggcg atggaccgcg acctggagat ccaccggctg aacgcccgcc
     4501 tgcggaccct tcgtcgttac ggtctggacc tgtgcctcgg gcacatcgtc accgcggacg
     4561 agccggagcc gatgtatgtc ggaaggctcg gcctcaccga cagcgcgggc cgccggctcc
     4621 tgcacgactg gcgctcaccc gcggccgagc cgttcttcgg agcgacccac gccaacccga
     4681 tgggactgac gagccgccgc agatatcgct ggacgcgtgg ccggatcagc gactactggg
     4741 acgaggtctt caccgcggac gagttcgacc gacactcagc tgcgctcgac gatcagtccg
     4801 ccttcatcgc cagccttggg cgcagccggt cgccgcagat gcccgacgtg ctcggcacca
     4861 ttcaggccga ccaggacgcg atcatccgtg cgagttcccg cggtgcgctc gtcgtcgacg
     4921 gcggtccggg gacggggaag accgttgtcg ctctgcaccg tacggcctac ctcctgtact
     4981 ccgaccctcg cctcggtcac gag
//
LOCUS       DATBFZ010000387         2860 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_1907437, whole genome shotgun sequence.
ACCESSION   DATBFZ010000387 DATBFZ010000000
VERSION     DATBFZ010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2860
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1907437"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1171
                     /locus_tag="VH419_11650"
     CDS             <1..1171
                     /locus_tag="VH419_11650"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY7044313.1"
                     /translation="VPYYRETWSESAVTGVREGQDLYRLPVLEPAVVRDIPAARLLTD
                     GVEPDACFPVRTSGSSGTPRTVYFSRADMNVIRGRYVWDMIMVGFRPWDRLGYLRFAP
                     IRRHRLERFGLLRSFHVDSTKPLPAQARDLIAARPTVLVGFPNLTLSVVEELERQRAR
                     SSGVRLVLFGGERTSPEARRYILDQLGARGCEMYACTEANVIARSCRRGALHLRTTDV
                     VVEVEHDDGSVSVADGAGQIIVTPLQAKAMPIVRYRLGDRVEIGPDDCGCGRGRRPIL
                     HTVSGRVDDRVLDRSGRAVNAQQIVTAVEGVSGISRLQLHQRIPGEIAIHAVIAAGSP
                     NDVVEQIEAAARPVSGQFEVTVRIVDDVEREASGKIRLVRRHEPSASSRSGTAHA"
     gene            1305..1850
                     /locus_tag="VH419_11655"
     CDS             1305..1850
                     /locus_tag="VH419_11655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044314.1"
                     /translation="MDGATEDRPSAEAPPARRWRADAVSRLTSTNAAGLLYGGIVTAA
                     TIAVASAHSERGLLVLVAVGGALVTYWLAHVYVRALTDRLAAGGLRLPHHLRHAAGHE
                     STVLLGGLPALVIFTVEVAIGIDVGTAGVVALWVSVFLLAGVGYLAAHAAGMRGRALA
                     GETAVAGLFGVFAVLLKSLLH"
     gene            complement(1869..>2860)
                     /locus_tag="VH419_11660"
     CDS             complement(1869..>2860)
                     /locus_tag="VH419_11660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044315.1"
                     /translation="AAGLPEPAMVNGVLQSPMEGTSMLYTFNGADLPERHDLQYFEMF
                     GNRGIYHRGWSAVTKHRTPWVMVGGVLPAFDDDVWELYDGTSDFSQARNVVAEQPEML
                     AKLQRLWLIEATKYNVLPMDDRTAERIEPTMAGRPTLIRGNSQLFLPGMGRLSENSVV
                     SIKNKSFAVTAEVEVSEDGADGVIIAQGGRFGGWAFYAKDGTTKFVYNVLGIQEFSTQ
                     ADQPVPTGTHQVRMEFAYDGGGLGKGGDVTLFYDGEAVGKGRVDATQPMVFSADETTD
                     IGYESGTTVTPDYTAHRSRFSGRIHWVQIDLGDDDNDHFIEPEERLRIAIARQ"
BASE COUNT          401 a          955 c          990 g          514 t
ORIGIN      
        1 cgtcccctac taccgcgaga cgtggtccga gtccgccgtg accggcgttc gcgagggcca
       61 ggatctgtac cggctcccgg tcctcgagcc tgccgtcgtc cgcgacatcc ctgcggcgcg
      121 actgctgacc gacggggtag agcccgacgc ctgcttccct gtccggacca gtggatcatc
      181 cggtacgccg cgcacggtgt acttctcgcg tgccgacatg aacgtgatcc gtggtaggta
      241 cgtctgggac atgatcatgg tcggtttccg gccgtgggac cgactcggct atctacggtt
      301 cgcaccgatc cgccggcatc ggctcgagcg gttcgggctg ctgcgttcgt tccacgtcga
      361 ctcgaccaag ccactaccgg ctcaggcccg tgacctgatc gcagcccgcc cgacagtcct
      421 ggtcgggttt ccgaacctga ccctcagtgt cgtcgaggag ctggagcgtc aacgtgcccg
      481 ctccagcggc gtacggctcg tgctgttcgg tggtgagcgg acgtcgcccg aagctcgccg
      541 atacatcctc gaccagctcg gcgcccgcgg ttgcgagatg tacgcctgca cggaggccaa
      601 cgtcatcgcg aggtcgtgcc ggcgcggtgc cctgcatctt cggaccaccg atgtcgtcgt
      661 cgaggtcgag cacgacgacg gctcggtctc ggtggccgac ggggcaggcc aaatcatcgt
      721 cacgccactg caggccaagg cgatgccgat cgtgcgctac cggctcggcg atcgtgtcga
      781 gatcggcccc gatgactgcg ggtgcggtcg cgggcgtcgc ccgatcctgc acaccgtctc
      841 cggacgcgtc gacgaccgag tcctcgatcg gtctggacgt gcggtgaacg ctcaacagat
      901 cgtcaccgcg gtcgagggag tctccgggat cagccggctc cagctccacc agcgcatacc
      961 gggggagatc gcgatccacg cggtcatcgc ggccgggtcg ccgaacgacg tcgtcgagca
     1021 gatcgaggcc gcggccaggc cggtgagcgg ccagttcgag gtgaccgtac gcatcgtcga
     1081 cgacgtcgag cgggaggcga gtggaaagat ccgcctggtc cgccgccatg agccgagcgc
     1141 cagcagcagg tcagggaccg cgcacgcttg atcactcggt cgctatcgag gagcaggcgc
     1201 accggcgctc cggcgtacat cggggagatc acgatcgggc ttgcactcgc cgccccatcg
     1261 ctcggatgcg cacatcgtca acaggtgacc gatagcgtcg gatcatggat ggagccaccg
     1321 aggatcggcc atcggcggag gccccgcctg ctcggcggtg gcgcgccgat gcggtgtccc
     1381 ggctgacgag cacgaacgcg gccgggctgc tgtacggcgg gatcgtgacg gccgcgacga
     1441 tcgccgttgc gtcggcgcac agcgagcgtg gtcttctggt cctcgttgcc gtcggaggcg
     1501 cgctcgtcac gtactggctg gcgcacgtct acgtgcgggc actgacggat cgcctggcgg
     1561 ccggtggcct caggctgccg catcacctcc ggcacgcggc cggacacgag tcgactgtcc
     1621 tgctgggcgg gttgccggcg ttggtgatct tcaccgtcga ggtggcgatc gggatcgacg
     1681 tcgggacggc cggcgtggtc gcgctgtggg tttcggtctt cttgttggcg ggcgtgggat
     1741 acctggccgc tcatgccgcc gggatgcgcg ggagggccct cgccggcgag acggcggtcg
     1801 ccggcctgtt cggtgtgttc gcggtgctcc tcaagagcct gctccactga cgagccggtg
     1861 agccgtcgct actgcctggc gatggcgatc cgcagccgct cctccggctc gatgaagtgg
     1921 tcgttgtcgt cgtcgccgag gtcgatctgc acccagtgga tcctgccgct gaagcgactg
     1981 cggtgggcgg tgtagtcggg cgtgacggtc gtgccggact cgtagccgat gtcggtcgtc
     2041 tcgtctgcgg agaaaaccat cggctgggtg gcgtcgaccc tgcctttgcc gacggcctcc
     2101 ccgtcgtaga acagtgtgac gtcgcctccc ttgcccaggc cgcctccgtc gtacgcgaac
     2161 tccatgcgaa cctggtgcgt tccagtcggg accggctggt ctgcctgcgt cgagaactcc
     2221 tggatgccga ggacgttgta gacgaacttg gtcgttccgt ccttggcgta gaacgcccag
     2281 ccgccgaacc tgccgccctg ggcgatgatc accccgtcgg cgccgtcctc ggagacctcg
     2341 acttcggcgg tcaccgcgaa cgacttgttc ttgatgctca cgacactgtt ctccgacagg
     2401 cgtcccatcc ctgggaggaa caactgcgag tttcctctga tgagggtcgg tcgcccggcc
     2461 atcgtgggct cgatccgctc cgcggtccgg tcgtccatcg ggagcacgtt gtacttggtg
     2521 gcctcgatga gccacaggcg ctgcagcttg gccagcatct cgggctgctc ggcgacgacg
     2581 ttgcgcgcct ggctgaagtc gctggtgccg tcgtacagct cccagacgtc gtcgtcgaac
     2641 gccggcagca cgccaccgac catgacccac ggggtgcggt gcttggtgac ggcgctccat
     2701 ccgcggtggt agatccctcg gttgccgaac atctcgaagt actggaggtc gtgccgctcc
     2761 ggaaggtcgg cgccgttgaa ggtgtagagc atgctcgtgc cctccatcgg ggactgcagc
     2821 acaccgttga ccatggcggg ctcgggaagt cccgccgcct
//
LOCUS       DATBFZ010000388         2162 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_266632, whole genome shotgun sequence.
ACCESSION   DATBFZ010000388 DATBFZ010000000
VERSION     DATBFZ010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266632"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..981)
                     /locus_tag="VH419_11665"
     CDS             complement(139..981)
                     /locus_tag="VH419_11665"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HEY7044316.1"
                     /translation="MRLDRVRAPASALALAAHPVPSLAVTVMTVALTAGAGNDAATCV
                     LVGVAVLSGQLSIGWSNDALDAERDQQVGRSDKPVAVGAVSTSTVLTAAFVALPVTVL
                     ASFALGWRAALAQLVMVACGWIYNLGVKSTVLSWLPFVPAFAALPAVATLALPDHPWP
                     PWWSLVAGGLIGVSGHLGNVLPDLAQDDVTGVRGLAHRIGPAWTAALGLGSAFAAVVL
                     VALAPGDSVTWWSWLGLAVAAVVTAVAFIVVRRRPSSEAAFYATTTVAAIGVTLLATS
                     PAFP"
     gene            1070..>2162
                     /locus_tag="VH419_11670"
     CDS             1070..>2162
                     /locus_tag="VH419_11670"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008355246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY7044317.1"
                     /translation="MADEATEVDVVVIGLGPGGEATATQLAQAGLDVVAVDRRLVGGE
                     CPYYGCIPTKMMIRGSDLIAEGRRIPEVSGTATVEPDWSPVAKRIAEEATSDWDDQIA
                     VDRLQDAGARVLHGVGRLDGPKTVVVGDERFVARTGVVLNPGTEPAVPPIDGLADTPY
                     WTNRDAVQLTELPDSLIAIGGGAISLELAQAFSRFGVDVTVLEVAPRLLPVEEPESSR
                     LIADVFAREGIQVLTGATISSVSYDDGRFTASVDGQSLMADKLLVAAGRRPNLSDIGL
                     ETVGLDPSARVLETDEAMRVESVDGLWAIGDVTGKGAFTHMSMYQSAVATRSVLGQEG
                     PPADYRAVPRVTFTDPEIGGVGMSEQQARD"
BASE COUNT          378 a          725 c          783 g          276 t
ORIGIN      
        1 gagcggctgg ggtctgtcaa aggcttccgc gtcctcgaga ctcggcaccg acacggctga
       61 tcgaggtggc aaagccggtc cgacacgccg gcgaactcca acgaaagttg ccacctcggc
      121 ggtggtcgag ggagaaggtc aggggaaggc gggggaggtg gcgagcaggg tgacgccgat
      181 cgccgcaacc gtcgtggtcg cgtagaaggc cgcctcggag gacggtcgac gacgtacgac
      241 gatgaacgcc accgccgtga ccacggcagc gacggcgagg cccagccatg accaccaggt
      301 gaccgagtcg cccggcgcca gcgcaaccag gacgacggca gcaaatgccg acccgagacc
      361 cagcgctgcg gtccacgccg gtccgatccg gtgcgcgagc ccacgtacgc cggtcacatc
      421 gtcctgcgca agatcaggca gcacgttgcc gagatgcccg gagacgccga tcaaaccgcc
      481 ggcaacgagc gaccaccacg gcggccacgg gtggtcaggc agtgcgagcg tggccacggc
      541 aggcaacgca gcgaacgccg ggacgaacgg cagccacgac agcaccgtcg acttgacccc
      601 gaggttgtag atccagccac acgccaccat gaccagctgg gcgagtgccg ccctccagcc
      661 gagtgcgaac gacgcgagca cggtgacggg cagtgccacg aaggcggccg tgagcacggt
      721 cgacgtggag accgcaccga cggcgaccgg cttgtcggag cggccgacct gctggtcgcg
      781 ttcggcgtcc agcgcgtcgt tcgaccagcc gatcgacagc tgacccgaca agacggcgac
      841 acccaccagc acgcacgtgg cggcgtcgtt ccccgcccct gccgtcagcg ccaccgtcat
      901 caccgtgacg gccaggctcg ggacgggatg cgcagccagt gccagcgccg acgccggagc
      961 cctgacccga tccagccgca cgccgcgcag tctatggcgg cggtcgcgcg ggactgcggc
     1021 aacgggtaag actgggcaat gcccacgcac gcccggcgaa ggagcggtca tggctgatga
     1081 agcaaccgag gtcgacgtcg tcgtcatcgg actcggaccc ggcggcgaag cgacggcgac
     1141 gcagctggcg caggccggtc tcgatgtcgt ggcggtcgac aggcgcctcg tcggcggcga
     1201 gtgcccgtac tacgggtgca tcccgaccaa gatgatgatt cgcggatcgg acctgatcgc
     1261 ggagggtcgc cggattcctg aggtgtccgg caccgccacg gtcgagccgg actggagccc
     1321 ggtcgcgaag cggatcgccg aagaggcgac ctcggactgg gacgaccaga tcgccgtcga
     1381 ccggctgcag gacgccggtg cgcgggtgct gcacggcgtg ggtcggctgg acggaccgaa
     1441 gacggtcgtg gtcggggacg agcggttcgt cgcgcgcacc ggtgtcgtcc tcaatccggg
     1501 caccgagcca gctgtgcctc cgatcgacgg gctcgccgac acgccgtact ggacgaaccg
     1561 ggacgccgtg cagctcaccg agctaccaga ctcgctcatc gcgatcggcg gtggcgccat
     1621 ttccctggag cttgcccagg cgttctcgcg gttcggagtc gacgtcacgg tcctggaggt
     1681 tgccccgagg ttgctgccgg tcgaggagcc ggaatcgagc cgcctcatcg ccgacgtctt
     1741 cgctcgcgag ggcatccagg tgctgacggg cgcgacgatc tcgtcagtct cgtacgacga
     1801 cggacgcttc acggcgtcgg tcgacggcca gtcgctcatg gccgacaagc tgctggtcgc
     1861 cgcaggtcgc aggccgaacc tgtcggacat cggactcgag acggtcgggc tcgatccgag
     1921 cgcccgggtc ctggagaccg acgaggcgat gcgcgtcgag agcgtcgacg gactgtgggc
     1981 gatcggtgac gtgacgggca agggcgcctt cacccacatg tcgatgtacc agtcagcggt
     2041 ggcgacgcgt tcggtgcttg gtcaggaagg cccgccggcc gactaccggg cagttccgcg
     2101 tgtgacgttc accgatcccg agatcggagg cgtcgggatg agcgagcagc aggcccgcga
     2161 cg
//
LOCUS       DATBFZ010000389         4257 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_1253952, whole genome shotgun sequence.
ACCESSION   DATBFZ010000389 DATBFZ010000000
VERSION     DATBFZ010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 4257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4257
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1253952"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(100..2010)
                     /locus_tag="VH419_11675"
     CDS             complement(100..2010)
                     /locus_tag="VH419_11675"
                     /inference="COORDINATES: protein motif:HMM:NF037016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEY7044318.1"
                     /translation="MSPGDVLLLGFDGVDKLAPGGPVRLASVDLGDAMAADATGDFYV
                     TNFDGDVIKFDHVTGEQTVVHHGISLVFPWRDLAVDAAGRVLGLFDEETGTALVRINP
                     NGSLTQLSDGQFFDDPDALAVQWDGSVIVSDGSTLLRVDPNSGQQKIIANLGARIRGL
                     DIRSDAQIYARTLRNGSTAPAIHQIDPVTFQHHVITSGGQLRDGGSNGMVFQDGTNLL
                     VAEEFDADRDQVFRVNTRTGAQKLVVPRDHGDDNDIAVVGQSQVPPVPLPVAVDDSFS
                     VLQSRTLTGQVLGNDSDPILHQKLTVAQASDASHGKLFLEKDGSFSYTPNPGYVGPDN
                     FHYRAVANGRTSARVATVDITVQPATPVANPENFFLTLEKGELDVPAPGLLANDTDPL
                     GQQLSAEVVGSPTSPNGFIGLAKDGSFFYFPFSGFIGIDCFHYHVVTVDGRTSAPSQF
                     CIQVVHNTAPSVVAEAGGGINASGTAGTVHLGVFDLQTANQDLTVTASSSNTALLPRS
                     RVKVSGPVGQIGQQRDVQITPVVGKTGIAVVTITVTDDVGATASVSVTVKVGGTGKDS
                     LVGTSGADLLLGAGGSDTLSAGAGVDLLGGGGGNDTLTGGAGPDVFRGHAGIDKVTDF
                     SAVEGDATLETE"
     gene            complement(2121..2294)
                     /locus_tag="VH419_11680"
     CDS             complement(2121..2294)
                     /locus_tag="VH419_11680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044319.1"
                     /translation="MHSKSTRAVGRFGGYVRRFGQGIRDVLFAPDVEPNLPRLSDYPL
                     RRTPSGSSGTGYR"
     gene            2513..>4257
                     /locus_tag="VH419_11685"
     CDS             2513..>4257
                     /locus_tag="VH419_11685"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HEY7044320.1"
                     /translation="MDLRLLGPVEVLVDDTQIDLGPRMHRALLALLALQAGERVSLEA
                     IIDGLWVDPPDSARATVQGYVYGLRRALEPDRSHRMPASVLAGADGGYRLQIPKESVD
                     VGRVRSLDVESAGLVRRRDVAAARTATTRALEAWRGEPLAGLTHPFAELARTSLEELR
                     LGLEQRLADIELESGRHVEVAARLVPLVAAAPLREPLRERLALALYRSGRQADALAVI
                     DEGRQILADELGLEPGTALAELRTAVLRQEPALDAPATPQAGTSPRPVPTPSTGGPAL
                     IERDDLLAQLRGLLETSVTTDGRLVMLGGEAGVGKTSLALALVDDVTASGVRGTDRVQ
                     VRRGHCDDLSVPAALGPVVEAFPEIESLLEHDDVVQPTLLRRVREQLSTTPTLFVIED
                     VHWADEATLDMLRFLGRRMAGTPVLIVATFRDDEVSSIHPLVRLLGDLPSSPYMVRVH
                     VPPLTEPGVRALILESGGDQDPHALFEQTGGNPFYVTELLATPADSVPGTVRDAVLAR
                     ASRLSADARIVLAAAAVLGQPADLPLLLAVSSRPAASVDECVTAGLLVDAGPGWDFRH
                     ALARFAIQESLLPAQR"
BASE COUNT          610 a         1448 c         1472 g          727 t
ORIGIN      
        1 accccgtgcc aggcacaagc cttgccgatc ggacccggga gaaggcggat cggcccggac
       61 gcaggttgtg cgtccgggcc gatcccattg ggggaggggt cactcggtct cgagcgtggc
      121 gtcgccctcg accgcggaga agtccgtgac cttgtcgatg ccggcgtggc ctcggaagac
      181 gtccggcccg gcaccgccgg tcagggtgtc gttgccgccg cctccgccga gcaggtccac
      241 tccggcgcca gcggagaggg tgtcgctgcc tcccgcaccg aggagcaggt cggcgccgct
      301 ggtgccgaca aggctgtcct tgccagtgcc accgaccttg acggtgacag acaccgatgc
      361 cgtggcgccg acgtcgtcgg tgaccgtgat cgtcacgacc gcgatacccg tcttgcccac
      421 caccggagtg atctgcacgt cgcgctgctg gccgatctgt cccaccggtc cactcacctt
      481 cacccgggac ctcgggagga gtgcggtgtt cgagctgctc gccgtgaccg tcaggtcctg
      541 gttcgctgtc tgcaggtcga acacacccag gtgcaccgtg cctgctgtcc cggaggcgtt
      601 gatgccgcca ccggcctccg cgaccacgct cggggcggtg ttgtggacga cctggatgca
      661 gaactggctc ggggccgagg tccgaccgtc gacggtgacc acgtgatagt ggaagcagtc
      721 gatcccgatg aagccgctga acgggaagta gaagaacgac ccgtccttgg ccagcccgat
      781 gaatccgttc ggggatgtcg gggatccgac gacttcggcc gacagctgtt gcccgagcgg
      841 atcggtgtcg ttggccagca gacctggcgc cggcacgtcc agctcgccct tctccagcgt
      901 gaggaagaag ttctccggat tcgccaccgg cgtcgccggc tgcacggtga tgtccaccgt
      961 cgcgactcgg gccgaggtcc ggccgttggc aaccgcgcgg tagtggaagt tgtcgggccc
     1021 gacgtacccg ggattcgggg tgtagctgaa ggacccgtcc ttctccagga acagcttgcc
     1081 gtgactggcg tccgaggcct gcgccacggt gagcttctga tgaagtatcg gatcgctgtc
     1141 gttgcccagc acctggccgg tgagcgtccg cgactgcagc acgctgaacg agtcgtcgac
     1201 ggccaccggc agcggcaccg gcggcacctg gctctggccg accaccgcga tgtcgttgtc
     1261 gtcaccgtgg tcacgcggca ccaccagttt ctgcgcgccg gtgcgggtgt tcacccggaa
     1321 cacctggtct cgatcggcgt cgaactcttc tgccacgagg aggttggttc cgtcctggaa
     1381 caccatcccg ttgctgccgc cgtcacgcag ctgaccgccg gacgtgatca catggtgttg
     1441 aaaggtcacc gggtcgatct ggtggatcgc cggcgcggtg gacccgttcc gtagcgtgcg
     1501 ggcgtagatc tgcgcgtctg agcggatgtc cagtccgcgg atccttgcgc cgaggttcgc
     1561 gatgatcttc tgttggccgg agttcgggtc gacccgcagc agcgtcgatc cgtcggaaac
     1621 gatgacgctg ccgtcccact ggacggccag cgcgtcgggg tcgtcgaaga actgtccatc
     1681 ggacagctgc gtcagcgacc cgttcgggtt gatcctgacg agggcggtgc ccgtctcctc
     1741 gtcgaagaga ccaagcactc ggccggccgc gtccaccgcc agatcgcgcc acgggaacac
     1801 caggctgatt ccgtgatgca ccacggtctg ctcacctgtg acgtggtcga atttgatgac
     1861 gtcaccgtcg aagttcgtca cgtagaagtc accggtggcg tcggccgcca tcgcgtcacc
     1921 caggtccacc gaagccagcc gtacgggtcc gccaggtgcg agcttgtcga cgccgtcgaa
     1981 tcccagcagc aacacatcac cagggctcac gaatggttct tctgcgttcg ccggtgtttg
     2041 cgaaaccgtc gacgtcaagc tgaccgcgag gacagcggca acggcggagc ccagcaggcg
     2101 cggcacgagg cgtgggcttc tcatcggtag cccgttccgc tgctcccgga cggcgtacgc
     2161 cgcagcgggt agtcgctgag tcgcgggagg tttggttcga cgtccggtgc gaagaggacg
     2221 tcgcggatgc cctggccgaa tcggcgtacg tagccgccga accttcctac agcccgggtg
     2281 gacttgctgt gcatggtgat ctccttcatg cccgggccgt ccgggcgctt ggtcgagcat
     2341 cggggccgcg actggggccg ctctgggacc gcgctgggac ggtgctgggg acggggctgg
     2401 gacgcctgga ctgagccgga gtgtgcgagt ggcgtcaccg gactgagacc gagttgcgac
     2461 atccgccgtt gccgggccta cgctccttgc acgagggagg caggtgagtg ccatggacct
     2521 ccgactgctc ggccctgtcg aggtcctcgt cgacgacacg cagatcgatc tggggccacg
     2581 gatgcaccgg gccttgctgg ccctgctcgc cctgcaggcc ggcgaacgcg tcagcctcga
     2641 agcgatcatc gacgggttgt gggtggatcc gcctgactct gcccgcgcca ccgtgcaggg
     2701 ctatgtctat gggctccgcc gcgccctgga gcccgatcga agccaccgca tgcccgcctc
     2761 agtcctggcc ggtgcggacg gcggctaccg cctgcaaatc cccaaggaaa gcgtcgacgt
     2821 cggccgggtc cgctcgctcg acgttgagag cgcaggtctg gtacgccgcc gcgacgttgc
     2881 tgcagcacgc accgcaacga ctcgcgcgct cgaagcgtgg cgaggcgaac cgttggccgg
     2941 gttgacccat ccgttcgccg agctcgctcg aacgtcgctc gaggagctgc ggctcggcct
     3001 ggagcaacgg ctggccgaca tcgagctcga gtccggccgg cacgtcgagg tggcggcccg
     3061 actcgttccg ttggtggcgg ccgcgccgtt gcgcgaaccg ttgcgtgaac ggctggcgct
     3121 ggcgctgtac cgctccggac ggcaggccga cgccctggcc gtcatcgacg aaggccgtca
     3181 gatcctggcg gacgagctcg gcctcgagcc gggtaccgca ctggcagagc tgcggaccgc
     3241 cgttctgcgc caggagcccg cgctcgacgc ccccgccaca ccccaggccg ggacgtcgcc
     3301 tcgccccgtg ccgaccccgt ccaccggtgg ccccgcgctc atcgagcgtg acgaccttct
     3361 cgcgcagttg cgcgggctgc tggaaacgtc ggtcacgacc gacgggcggc tggtgatgct
     3421 cggcggtgag gccggggtgg gcaagacttc gctggccttg gctctggtgg atgacgtgac
     3481 ggcatccgga gtccggggca ccgatcgggt gcaggtgcgc cgtgggcact gtgacgacct
     3541 ctcggtccca gccgcgttgg gtccggtcgt cgaggcgttc cccgagatcg agtcactgct
     3601 cgagcacgac gatgtcgtgc agccgacgtt gctgcgtcgc gttcgtgaac agctctccac
     3661 gacccccacg ctgttcgtga tcgaggacgt gcactgggcc gacgaggcga ccctggacat
     3721 gctccggttc ctgggtcgac gaatggcggg gacacccgtg ctcatcgtgg cgaccttccg
     3781 tgacgacgag gtgtcctcga tccatccgct ggtacggctc ctcggtgacc tgccgtcctc
     3841 gccgtacatg gttcgagtac atgtgccgcc cctcacagag ccaggagtcc gggccttgat
     3901 cctggagtcc ggtggggatc aggatccgca cgccctgttc gagcagaccg gcggcaatcc
     3961 cttctacgtc accgaacttc tcgccactcc tgcagacagc gtgcccggaa ccgtgcgcga
     4021 cgcggtgctc gcgcgggctt cccggctctc ggccgacgct cgcatcgtgc tggctgcggc
     4081 cgccgtactc ggccagcccg ccgacctgcc actgctgctc gcggtctcca gccgtcccgc
     4141 ggcttcggtc gacgagtgcg tgacggcggg gctgctggtc gacgccggac ctgggtggga
     4201 cttccggcac gcgctcgcgc ggttcgccat ccaggagtcg ctgctaccgg cgcagcg
//
LOCUS       DATBFZ010000390        25329 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_6372273, whole genome shotgun sequence.
ACCESSION   DATBFZ010000390 DATBFZ010000000
VERSION     DATBFZ010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 25329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25329
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6372273"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            489..692
                     /locus_tag="VH419_11690"
     CDS             489..692
                     /locus_tag="VH419_11690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044321.1"
                     /translation="MIDPGNRVVVLGRLTVPQVLRLIAEIHQAKLAQSGSDTAQLELL
                     GILAILFGIGSVWLSHTRRRTAV"
     gene            1057..1314
                     /locus_tag="VH419_11695"
     CDS             1057..1314
                     /locus_tag="VH419_11695"
                     /inference="COORDINATES: protein motif:HMM:NF023174.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3303 family protein"
                     /protein_id="HEY7044322.1"
                     /translation="MLFHIKQSHAPQDCPYGKGGSKSLFDEGNPDVTIRGYWLAFPQH
                     TTFLVVETDDVRHLQQFLKPGAAVTTSEITPVSDEPVPPPA"
     gene            complement(1384..2427)
                     /locus_tag="VH419_11700"
     CDS             complement(1384..2427)
                     /locus_tag="VH419_11700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044323.1"
                     /translation="MRRVVVAAGLAIVGSAPFALVSNAAAADSVKPAVVGLVDRQRTI
                     DDNWAGVIRGVVVQVDWAALQPTRDGPIVTNNPIDAAVTAVQAYNQRHPDATLGIKLR
                     VMAGRGSPQWLKDRAGYQFVQSPVDQSSGTVPFWWKRQVDTAYRALQQALAARYDGIP
                     EIREVQITRCTVLYDEPFMRITGDRVALTAFVANGLTRAADQTCYKQEVQAHAVWHQT
                     RSVLALNPYQDPATVPITDVDFTVSMAKQCRRLLTNRCVIENHSIRETSLGADYDKLY
                     KKMTVLGRPMAMQTNRPDVLGDLGVVLRRCVGMGAEAVELPWTYRQMTPEAFRAAYGD
                     VISALDPAVVAVP"
     gene            2578..3384
                     /locus_tag="VH419_11705"
     CDS             2578..3384
                     /locus_tag="VH419_11705"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY7044324.1"
                     /translation="MPTVQANGIDVNYEVQGEGEPMVLIPYLAADQACYAFQVGEFAK
                     QFTCYSVDLRGAGLSGKPEGVYTTELFADDVAAFMDAAGVDRAHVFGLSLGAATAMWL
                     AGKYPERVKSLALCSAWPRSDPFLEVIVEGWRIMAKALGSVADMVITGIFPWCFTPEL
                     YASRPEFVASLADFVRGRPMPTVDEFERQSGAVLAHDATAVLGAIEAPTLVMFGEHDL
                     VTSTRFAAPLTSAINGAELAVFEGCSHAPIYEDIEGFNQRTLDFLQRHTG"
     gene            complement(3668..3949)
                     /locus_tag="VH419_11710"
     CDS             complement(3668..3949)
                     /locus_tag="VH419_11710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044325.1"
                     /translation="MRLRIGIALWLLSWFPFAAIVRADAAERAAIWTVQVIIGIIGLA
                     LAGTAFATLVKKVGWRHAPALAWHSLLHGQVDPHDPSAAAAEPPSESLT"
     gene            complement(4028..4615)
                     /locus_tag="VH419_11715"
     CDS             complement(4028..4615)
                     /locus_tag="VH419_11715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044326.1"
                     /translation="MVADLEALGEAINGLAALDIEALGDEELHALVVGVQRDRARLGV
                     VAGELVGRWDARQVWANDGSRRGAPVERNELRSGGCGHGTASSPPAAVAPGHGVRGPE
                     GDLSLDHLDLAGRANQAHRAACFARDEVAVVEQCRTLRFSNRRLECDTHNRNTDRHHH
                     HAVPLPTRPVTPLDQIRARLRWQIQHDKLTRGPAP"
     gene            4722..5624
                     /locus_tag="VH419_11720"
     CDS             4722..5624
                     /locus_tag="VH419_11720"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEY7044327.1"
                     /translation="MLDADLVLEGGGVKGIGLVGAISVLEERGYRFHRVGGTSAGAIV
                     GSLVAAGMTAQRMQDVMRSVAYRRFRDPNLLDLFGPGGQALSVLVHNGIYRGDYLKSW
                     LSEQLSDLGVRSFEDLVLVDPGSSLPPERSYRLVVMTSNLSDNRLTRLPWDYPGLGVT
                     TDHQLVVDAVRMSMSIPFFYEPVNFRGRCFVDGGMLSNFPVDVFDRTDGLPPRWPTFG
                     IKLSGRASSDSARAPRGVGGPLGMVKAMVATMTSFYDRMHLDDPGVLDRTMFVDCGHV
                     QPTDFDLTKSDQDMLYENGRRAAE"
     gene            5777..7516
                     /locus_tag="VH419_11725"
     CDS             5777..7516
                     /locus_tag="VH419_11725"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY7044328.1"
                     /translation="MRDSAAMRYAFDDCELDTGVFELRRAGVPVKVEPQVFDVLTYLV
                     LHRDRVVSKDELLEEVWGHRFVSESTLTSRVKAARQAVGDDGTTQRCIRTVHGRGYRF
                     IAPTVETADDATVANRLDRDAAAEATDASNAAAADAATVADAATAADAATAADAAAPI
                     RYAQSDGLNIAYQITGGGELDLLLVAGFVSHLKLDWTERRHAHFLHRLGSFARLIRFD
                     KRGTGMSDRPGSLPTLETRMGDVVAVMDAARSPSAVLFGYSEGGPMSILYAATHPGRV
                     DGLVIYSSYARRLWAPDYPWGFRPDERARYAQQIEQDWAWEADMRQMCPNADAELAGW
                     WGERCRAATSPGAARALIEMNSLVDVRDVLSAVQAPALILHRRDDRDANVEEGRYLAA
                     HIPGATFVELAGTDHFVAVDPDQILDPVEEFVRGLAPSAPTESSLTTVLAVHVEEGID
                     LAWVRGMLQAELVRHRGTPAASDDTAVLATFDGPGRAIRCALALTQQAARAGLHLSAG
                     LHTAELARRGTQVWGDGTSVAQAVADQATAGEVWVTATVRDLTAGSGLSFERRETLGL
                     ASLGRQLELDAVR"
     gene            complement(7523..12322)
                     /locus_tag="VH419_11730"
     CDS             complement(7523..12322)
                     /locus_tag="VH419_11730"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044329.1"
                     /translation="MRSARGRCVAHIAAAMTMVPLAWAAASVPSTPPAAAAVTNPPIA
                     DSCGVNVTLVLDASGSIQSSGAVENVRGAAAAFLDALKNTSSAARVTQFGTVGAQLAP
                     TTLVDDTSLGQNGVLTRALAGYYNPIPTRPANVNFYQYRGTGSITSASSFTLSNSGTA
                     NPTANTQYTNWDQALDQAGTGTLPKLVVFVTDGDPTAYDFGQPGDPFAAPHVAYNTNR
                     GQADATTLARAVDEADQIKTAGTRMLAVGVGTALSSPASVARLVAVSGPQVVDDSELA
                     SITSLNQVDVATVHDFANLAKFLRTVVLQLCSPSLTIRKLAQSANSADYVPAPGWTMQ
                     TTPTVPGGTFNWILPDTTPATSKTLVTDANGFAQFQWEPVPSTLHSQATVRETLRPGF
                     VAGRPNASDFRCELKDVDGNVRVVEGELDATGAFTLNPIADEIVTCTVWNSFNYQPAI
                     AVTKVNTPSEVRGDLNPPATVTSNYVVTNPGNTPLSNVTVVDDLCGAASPVPPTGFNA
                     GDTNHDGKLDTTESWQFTCTRPVLQSRLRSAAPVIVNTATARGTDPTGTNVTAQAQDD
                     VAVFTPAIELTKLVNGQPVVTVASGTTVTYTYAVANAGNTPLGSVALVDNTPPCQAPT
                     RGADGPGNNDTTLDVGEIWHFSCSAAPTAAVTNTATVTGVPLNPNNANAPFPNPNPPV
                     VSIDRAAVEVVHPGITLTKSADPTVVVLPPAGTAQPVTYTFVATNTGTPELNRPGATT
                     AGPAKDPGWVTDPHCTSPATFVSGDTNNNSLLDPGESWTFTCPGLVNTPTENVANIVG
                     QPSDATGAPLPGVPTVNDDAEAFVDVLRPGIAINKLALVPVVVDPAAPVVVGPDQPRR
                     PAQYSYEVTNTGDVPLALNPNPPVENPVICGPLTFEGGDTNADGLLDVGETWDYSCNT
                     LLQRSQGTPPPTGAESAFVTNHVSVTGTPSVSGTLHPDATVSASDQAQVLVTEPSIHI
                     TKSASPPVVEVGGAVTYTFVVSNTGDVGLNVLGPNDDQCSPLVPAAGNDANHNGFLDG
                     ADSGAPESWTYTCTREVGLPTPPATTNDNTVTVGGVDGLGNLYTSTATASVRVLDPAI
                     DLVKTVSSELVPSGRQVTYGFDATNIGTSPLPADDVLANVTLADSSTPATPACASPTF
                     VGGDTNGNNQLDRVPAEVWHYECTATITQPTHDIAIVKGTAGLGFNPQVPIDVFAADF
                     AFTRPFTPAIDVTKTASPTLLLGSGVVTYTYTVRNTGNVPLGDVKTRITDNTCSPLTY
                     VSGDTDGDGLLDTPNSIFEDAANEIWTFTCKVTLDKTTTNTVTVTGTPTDMVGAPLCG
                     AQTPNPDPCDATDTANATVTVVAPGTITIVKKTTAPSTATFPFALGTTQFTLASGASR
                     TFTPLAPGPYKAVEGPTSGYALTGLACTDPSGGTVVDTAHATANINLAAGETVVCTFT
                     NTRVPKADVVITKSVSSGMVTPGDEVTFTLLVKNLGPDTAEDVVVTDLIPAGVTVLQV
                     PAGCVLKGSVLTCAVGDLAPGASTKIVVPVRVTAVGTFTNLASVDAKTVDPNPDNNHS
                     FANVVGAATLPPTGSEVMRIVRLGWQLLFVGAVLAAVGFKRRRRRLA"
     gene            complement(12451..13206)
                     /locus_tag="VH419_11735"
     CDS             complement(12451..13206)
                     /locus_tag="VH419_11735"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HEY7044330.1"
                     /translation="MSGTVRVRLLGRLGVERGDRWLDGAALPGRQGRIVLAYLTLHHH
                     AVARDELADLVWPDALTASWERDLSAIVSKLRAALESIGLGSALQSASGSYEIRLPPD
                     STVDVQDAVRFVEDAEAAWRLGNRDAAVPAAMTAASLARRPFLPAEHGLWVERRRDEL
                     QATMLRALDVLVQGLTGGPFEHDAVRYANEALALDPYRETMYVRLMRLHMQRGSRADA
                     LRAYEQCRSTLSRDLGVTPSAQTEAAYQEVLGA"
     gene            13274..13423
                     /locus_tag="VH419_11740"
     CDS             13274..13423
                     /locus_tag="VH419_11740"
                     /inference="COORDINATES: protein motif:HMM:NF018096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1059 domain-containing protein"
                     /protein_id="HEY7044331.1"
                     /translation="MTKVINCDCGRVLRAATDDELVVAVQQHAREAHGMELTRDQVLS
                     LAVPE"
     gene            13450..14334
                     /locus_tag="VH419_11745"
     CDS             13450..14334
                     /locus_tag="VH419_11745"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY7044332.1"
                     /translation="MTVHYRSAAVAGVQVAYREAGNAANPPLLLLHGFPASSFSYRHL
                     MTALADRFHLIAPDYPGFGNSPAPNPDAWPYTFDHLADTVEGLVDHLGLSHFGLYMHD
                     YGAPVGFRLALRRPIAVDFLVIQNGNAYEEGFTGVWDGMRNALWQNRSPETEKPLHAF
                     FEPDGIKWIYTGGTRDPERISPDNWNLDLAVLAKPNARRINLDLFYDYRTNAELYPVW
                     QEYLRRHEPATLIVWGANDPIFRSEGAEAYLRDVPHAELHFLDSGHFALEDHGEEIAG
                     LIRAFHTTRVARAVSSAA"
     gene            complement(14351..15022)
                     /locus_tag="VH419_11750"
     CDS             complement(14351..15022)
                     /locus_tag="VH419_11750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044333.1"
                     /translation="MNTIAVARTDAGIASIERTVGATVVATAVGTTRATVRTVMRPTS
                     RLTVPPSVRRFVTAVRSRLAPTADGLADVPGTKIKTRWLLAGWAALSAVSAIGTAITP
                     ALIHFPLLMMAFTPRLPILVYAVKSHVSPLAFFAIAVPRMLIGDPIHLLLGRRYGSRF
                     VPAKARRLMVKLGVVGVVLRPTGNVLSAAGAVGMTTRRVMTADLVGTLALLLTIYTGT
                     RTIFG"
     gene            complement(15157..16449)
                     /locus_tag="VH419_11755"
     CDS             complement(15157..16449)
                     /locus_tag="VH419_11755"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEY7044334.1"
                     /translation="MLTAGGGGTGKRTLLLGLLALVPALLVAILLVHQPSYGDWHLYA
                     TLRLPVAGAALVLSAAAVASFALGPDRLRGAALGGLAWAAVAALIAIGPFLEASPRAR
                     LYALDARTGAVKWVNARAGESPMVIAGHLYVTDPGDRALVALDVASGKERWRDRLRPG
                     HSRSRALPRDATLVAATANGGVIVDATTGGTGAGSSACVPVDRGPVLVADGTSAYATT
                     DRQCQDQGATAGYRIGADDSTVFLVDRAKDFVRAVDARTGAERWRVSVRAAQLPVVLD
                     SRIAVASGTGGPLVVLDADTGQTVREISFAGERIRATAGADDMIYVYTASPVAGGDQS
                     GTVVALDAHTGALRWRSSLRSDEAPASGLPALGAYGGGVVVAGGPRVVDLDAADGAER
                     WSVDVTELGQSRRYALAGSSQRVTVADGRVFLSTSASL"
     gene            16625..18790
                     /locus_tag="VH419_11760"
     CDS             16625..18790
                     /locus_tag="VH419_11760"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEY7044335.1"
                     /translation="MTKSAALARAVVAVAIATTVAACVLAVGQSGPTGVDAWSGGLAG
                     FRLWTFVALVPVVVLTVVVHLHRPDHALGRLLAVASALLGLSTCAGIWLRTTDVQTLR
                     DGVAIAGAVFDIAAIVAVGALLALFPSGRGPRPFERLLLWAAGGFCVAVALLLLGAPL
                     RSVATATPPWSPLQVPSLLFFGGRGQGFLAFAALLPLVGIGLLTWRSPAMDGEERIHL
                     RWLALAGVTAASVLALVHLVRRLGLLQLGGDFYGVAPRVIAWLLVAAAVTVTALRPDV
                     ADAAAAVRRVAVAAALVVTLGLAGGLAWYATGVSTGTWLPGAPALAAGLTVAALFFPA
                     REAFERAADRWLFGDAIGDAQLLSAFGQAAERTAGLDGLAELIATTARRGLHVMWATV
                     SLHDPERTENAPTAEAGLVPDAPVPAASVELAIGGDVVGRLDCGPKRRGSLTDHDRQL
                     LAALAREAALDLQNARQASALAEQLVQIRRQAEELTASRTRIVQAQEAERRRIERNIH
                     DGAQQELAALLAKLRLAQNQLGRDPDTAERTIEEAQHDVRRALDDVRSLARGIHPAVL
                     SDRGLLEAIESSLTRLPLEAIIEADPALRARRFPDEIEGAAYFMCSEALANVLKYASA
                     TRVEVVLAVHDERLWLRVTDNGIGFDPQRVDGTGLPNLADRVEALGGRLRVQSTSGAG
                     TTVALELPVVLRQPASIVEPATVGHPGMDGQPAMSDWPA"
     gene            18787..19470
                     /locus_tag="VH419_11765"
     CDS             18787..19470
                     /locus_tag="VH419_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020575042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY7044336.1"
                     /translation="MNAPIRLVIAEDNFLFREGTRRLLEDSGELEVVAAVGDADELLQ
                     AVDALEPDVVITDIRMPPGHHMEGIAAAHTIREVHPSIGVVVLSQHAEEGYAFELLRD
                     GTAGLGYLLKERVGDLDELFRAIREVMAGRSVIDPKVVEGLVARQARVSTSAVHKLTP
                     RERDVLGEMAQGKTNAAIGESLHLSLSAVEKHVNAIFMTLGLTEEAQVNKRVAAVLLF
                     LRDSGLTPA"
     gene            complement(19487..20491)
                     /locus_tag="VH419_11770"
     CDS             complement(19487..20491)
                     /locus_tag="VH419_11770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044337.1"
                     /translation="MRPPQYLKNVRARADGVVKLADAGRDWAQRTIVWRVWERMLENE
                     FVDRSVALGAKAFVSFFPAIIVLASFAPPSIRTSIGQAIVRHGGLTGDGLSTFNAAFG
                     TSDSIRRATGVLGLIFTFFYVSSFTSALQRVYVRAWRRPPASKVSSYALGAVWLAGIF
                     AYLSMIGGMRVLLGHGPELAVFALLALAGSIALWWITPWFMLSRKVRLRVLFPTGLVT
                     GVALSLYGATAFLWMPRTMSQNQAQFGFFGVALSLVTWFSGSAIIVVIGACAAPVLAE
                     DRGFLGRLSRGSDPAVLVPGAKPSFGAPVRAPTLIDAIRLRTTDDEVEAQPTIETPEP
                     "
     gene            20569..20943
                     /locus_tag="VH419_11775"
     CDS             20569..20943
                     /locus_tag="VH419_11775"
                     /inference="COORDINATES: protein motif:HMM:NF023390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS/SEC14 domain-containing protein"
                     /protein_id="HEY7044338.1"
                     /translation="MIERMSDVSEGIIGFRFSGQVTKAEYTETVLPALQAAIKGDGRA
                     RTLVVIDEGFEKFEPGALWEDVKFGLGSGITHLSKWERTALVSDKDWARHAITLLGWM
                     VPGDVKIFPLAQLEDAKAWVAG"
     gene            21028..22389
                     /locus_tag="VH419_11780"
     CDS             21028..22389
                     /locus_tag="VH419_11780"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HEY7044339.1"
                     /translation="MGRPPDDRFRIFISHKHEDHALALATHQALESLTPTVECFVSGV
                     DIVAGSDWNREIRTQLAQSHLLVLLFTQPTQTWDWCLYETGLFTRFEADEVTAVVSIF
                     SADSGAPRVLANLQGVEARHEAVNRFLTALCKETWRVSDDWRLGALAPRVKPAQIERA
                     AAKIVNAFPHGSRDDSPYYPCHRVVLDLRAIDEIGDHIPEEARVIEGPNDTTGFTLSL
                     FNMADGRRHLTWRDLLVAVDGLEAGWRAQLDRRFVCALNEQLFTPITATLRAWNQGRR
                     HQRLLKPVLYRILRAPVPAGSPPDARGRPTGVTVVFDPQLAPTQVGGPALNLVRINAR
                     FGVEVFDEFVGTVRHRVADGLDVFVDIGEAIRLVYEEADRFGVFDENSLRRAYGPDYE
                     SKGIEAMGREWIDSLERLKKGLEERDADLVEEQLAALRQLNRSFSTASTERYLDLLRA
                     GAS"
     gene            22500..22880
                     /locus_tag="VH419_11785"
     CDS             22500..22880
                     /locus_tag="VH419_11785"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY7044340.1"
                     /translation="MSARVVHFEVPFDDGDRARSFYREVFDWQIQAVPEIGYNMVSTG
                     PTSDQGMPAEPGFIGGGMLQRQTPVNAPVITLQVDDIDATLVAVEKHGGAKVGDKMPV
                     GDMGFAAYFNDSEGNLMGLWQNAG"
     gene            complement(22881..24329)
                     /locus_tag="VH419_11790"
     CDS             complement(22881..24329)
                     /locus_tag="VH419_11790"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY7044341.1"
                     /translation="MSDYTAVVIGAGPAGHTCAVRLAQLGARVAVVERDFVGGICTNW
                     GCTPSKSMIESAKIARNVREAGRYGVNVSSFWVDFEKVAARRDEVILRSRGIVSRLLE
                     HHGVQVFQGEAQIVSPGRLRIRQGKLGLDGLEMSYTGTETMIEAEHIVVATGSRPLMP
                     SWAPPDHPYIVSSNRLITIGELPESLTVVGGGVIGLEFATIFANMGTRVTVVELQDRL
                     LGTLDPEISHALTEELNLIGVRTRTGHRVISVEEGYVRAAHGATGDIVDIPGRAVLVA
                     IGREPIVDAPSFDHLGVEHSTKGIGVDAQLRTSVANIWAIGDATGKSILAHVGMQQAL
                     VCADNIMTEPGRPRRSMEYGVIPSVVYSIPEVVAVGEVPDGSDDAVTAFKVPFSANLR
                     ARIESYEEGFVKIWVRDGKVVAAQAIGYYVSELMQELTDMIALGTPVHAVADIIHAHP
                     TYSEITRSVLELSLGRATDFLMPERSARGETS"
     gene            complement(24414..>25329)
                     /locus_tag="VH419_11795"
     CDS             complement(24414..>25329)
                     /locus_tag="VH419_11795"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY7044342.1"
                     /translation="VVYAASFASATAFVFSDAAVFGALPALIGDQRLPAANGLLTSIQ
                     SAALMIGPALGGLLATTIGAASAVWFDVASFFIAAAVLGAIRAPFSLHRVPEPRSAGL
                     GEQVRRGLRFIRQERTILTLIVIGFGNSFAFGVMLGLVVPYAVDRLGVPDNSVRLGLL
                     FTADALGALLAGLTFARIYARDRIRWLTPLCLGVSAALLVLLTANTSYALALVLTIAF
                     AFAVSTTISVGITYRQMASPDELRSSVNVVGRMVAWGGQPFGAAVGGIVAQTAGVPTA
                     LGMAAGVMTASAIGAVVLLGHRRQGALA"
BASE COUNT         3954 a         8618 c         8822 g         3935 t
ORIGIN      
        1 cgttcgacta ctccgacggg catttccccg gcctgaattg gccgggcggc gcggacatcc
       61 tcaacggcgg ctgcgccgtc cacgaagtcg aggtgccacc agaggagacg accaccacga
      121 cgacgccccc gcctcccaca acggaagcca ctaccgctcc cacgacgtcc acccctgaaa
      181 ccacggcagc cacgaccacg acgacagtgc ccgccgggtc cccgacgacg tccacgaccc
      241 cgacgacagc gaccgccccc acggtgaccg ctggggcgag cacatcggcc cctaccggcg
      301 gggcgaccac aagcgcgcct ccggtcaccg gcggtgccgg accgacaacg gcaccgcccc
      361 tgggagggac gacgatcgca ccgaccccgt ccaccctcgt accaccgaca accgcaccgc
      421 catcagaggt cccgccgcca gtgaccgaac caccgccagg tgcgccacta cctcaagtcg
      481 aggcaatcgt gatcgacccc ggaaacaggg tcgtcgttct cggccggctg accgtgcccc
      541 aggtgctcag actgatcgcc gaaatccacc aggccaagtt ggcccaatcc ggcagtgaca
      601 ccgcccaact cgaactgctc ggcattctcg ccatcctgtt cggtatcggg tcggtgtggc
      661 tctcacacac cagacggcga acggcggtct aacgccccag cgtgaggtcg cctacgccga
      721 tcgtcgttgc tgacacgccc actgtccccg gtccactggg agctcgacga ccacggcctg
      781 gggcggcgtc ctctgtcctg gggaggatcc aacatgcggg ggcctcgaag tccgcgccgc
      841 accaaggtcg gctgactctc ggcactccag tggcccccga cccacccggg tcggcggcct
      901 ttcttgttgg tcgcatctgc ccgaaccaca gttggagcga tgggcctacg ccttggcgcg
      961 tgagctcgcg cacgccatga cggtcagcat cagcggccac atggcgacgc tctagagtcg
     1021 ttcgccgaga cgccaccgag agggaggacc gcccggatgc tcttccacat caagcagtcg
     1081 cacgcacctc aggactgccc gtacgggaag ggcggctcga agtcactctt cgacgagggc
     1141 aacccggacg tcacgatccg cggctactgg ctcgcctttc cgcagcacac cacgttcctg
     1201 gtcgtggaga ccgacgacgt tcgccatctc cagcaattcc tgaagcccgg ggccgccgtg
     1261 acgacctcag agatcacacc cgtcagcgac gaaccggtgc cgcctccggc atagcgcagc
     1321 cgtctgcgag cggtgtttgg actgcgatcg ggatcgtccc agccgaggtg aacccgcgtt
     1381 tgttcacggg acggcgacca ctgccggatc gagtgcgctg atcacgtcgc cgtacgcggc
     1441 tcggaacgcc tccggcgtca tctgtcgata cgtccacggc agttcgaccg cctccgcgcc
     1501 catgccgaca caccgacgca gcaccacgcc gagatccccg aggacgtctg gccggttcgt
     1561 ctgcatcgcc attggacggc cgagaacggt catcttcttg tagagcttgt cgtagtcggc
     1621 tcccaggctc gtctcacgta tcgaatggtt ctcgatcacg caacgattcg tgagcagtct
     1681 gcgacactgt ttggccatgc tcaccgtgaa gtcgacgtcg gtgatgggga cggtggccgg
     1741 atcctggtag gggttcagag cgagcaccga ccgtgtctgg tgccacacgg catgggcttg
     1801 cacctcctgc ttgtaacagg tctggtccgc agcccgagtc aagccgttgg cgacgaacgc
     1861 ggtgagcgcg acgcggtcac cggtgatccg catgaacggc tcgtcgtaca gcaccgtgca
     1921 ccgggtgatc tgcacttcac ggatctcggg aatcccgtcg tagcgagcgg ccagcgcttg
     1981 ctgcagcgct cggtaggcgg tgtcgacctg ccgcttccac cagaacggca cagtgcctga
     2041 gctctgatcg accggactct gcacgaactg gtaacccgct cggtccttga gccattgcgg
     2101 ggagcctcgc cctgccatca ctcgcagctt gatgcccagc gtcgcgtcgg gatgccgttg
     2161 gttgtaggcc tggacggccg tgaccgccgc gtcgatcggg ttgttggtga cgatgggccc
     2221 gtcgcgagtg ggctggagcg ccgcccagtc gacctgcacg acaacgccac gaatcacacc
     2281 cgcccagttg tcgtcgatcg ttcgctgccg gtccaccaac cccaccaccg ccggcttcac
     2341 cgagtcggcc gccgccgcgt ttgacacgag tgcgaacggc gcgctgccca cgattgccaa
     2401 ccccgctgcg accacgaccc gccgcatgca gcacaactgt agtcgcgtcc ctggagaacg
     2461 cttgcgtctg catcgaccgc gctgacatcg tgccatgacg cgctctggtg gaagccgtgt
     2521 ggatgcgtga tgatggcgat cgccgaagcg cgcgcgtgcg gcgagaggag gacgaagatg
     2581 cccactgtcc aggcgaacgg gatcgacgtc aactacgagg ttcagggcga gggcgaaccg
     2641 atggtcctga tcccgtacct cgccgccgac caggcgtgct acgcgttcca agtcggtgag
     2701 ttcgcgaagc agttcacgtg ctactcggtc gacctgcgag gcgccggtct ctccggcaag
     2761 ccggaaggcg tgtacacgac cgagctgttc gccgacgacg tcgcggcgtt catggacgcg
     2821 gccggcgtcg atcgcgccca cgtcttcggt ctgtcgctcg gcgccgcgac cgcgatgtgg
     2881 ctcgcgggga agtacccaga gcgcgtgaag tccttggcgc tgtgcagcgc ctggccacgg
     2941 tccgatccgt tcctcgaagt catcgtcgag ggatggcgga tcatggcgaa ggccctcggg
     3001 agtgtggccg acatggtcat caccggcatc ttcccgtggt gcttcacgcc agagctctac
     3061 gccagccgtc ccgagttcgt cgcgtcgctg gccgacttcg tgcgcggtcg accgatgccg
     3121 accgtggacg agttcgagcg ccagtccggc gcggtcctcg cccatgacgc caccgccgtg
     3181 ctcggtgcca tcgaggcgcc cacgcttgtc atgttcggcg agcacgatct cgtcacgtcg
     3241 acgcgcttcg ccgcgccgct gacgtcggcc atcaacggcg cggagctcgc cgtcttcgag
     3301 gggtgctccc acgccccgat ctacgaggac atcgaggggt tcaaccagcg gacgctcgac
     3361 tttctccaac gccacaccgg ttgaccagcg gcgctgggac cgcctcacct gtttgggcga
     3421 ggccgggtgc gggcacttga agcatctatg ccgaggtaca ccgtcgcgat cgagaacgag
     3481 atccggacgt atcgacgcac gagcaccacg ccgcgtcggg gcgatcatcg cctggctcag
     3541 acgaaggtga acgcgcgacc cgcgcaggac cgtcgtcaga aggctctcag cggacgcgct
     3601 cctgcaggcg gccggaccgc ggctgttcga ggctcgaggc gcgcggcgta gcgactccgc
     3661 cgagcgatca ggtcagagat tcggagggcg gctcggcagc ggccgcactc ggatcatgcg
     3721 gatcaacctg gccgtggagc agtgaatgcc acgcaagcgc gggcgcgtga cgccatccca
     3781 ccttcttcac cagcgtggcg aacgcggtgc cggcgagcgc caggccgatg atgccgatga
     3841 tcacctggac ggtccagatg gccgcccgct ccgccgcatc ggctcgaacg atcgccgcga
     3901 acgggaacca cgacagcagc cacaacgcga tcccgatccg caggcgcacc gtgagatcat
     3961 cgttccgacg cgcccggttc gtcagcgcgg gctgagcgtg ctcgtcggcc tgttcagacc
     4021 tcgatggtca tggggctggt ccgcgtgtga gcttgtcgtg ttggatctgc cagcggaggc
     4081 gggctcgtat ctggtcgagt ggggtgacgg gccgggtggg gaggggtacg gcgtggtggt
     4141 ggtggcggtc ggtgttgcgg ttgtgggtgt cgcattcgag ccggcggttg gaaaaccgca
     4201 gcgtccggca ctgctcgacc acggccacct cgtcgcgggc gaaacaggcg gcccggtggg
     4261 cctggttggc ccggcccgcc aggtcgaggt gatccaacga caggtcacct tcaggaccgc
     4321 ggacgccgtg gccgggagcg accgcagctg gcgggctcga cgcagttcca tgcccgcagc
     4381 cgcccgagcg cagctcgttt ctctcgacag gcgcgccgcg ccgcgacccg tcgttggccc
     4441 acacctgacg ggcatcccaa cgacccacca gttcgcccgc caccaccccc aaccgggccc
     4501 ggtcccgctg cacccccacc accagcgcgt gcaactcctc gtcacccagc gcttcgatgt
     4561 ccagcgcggc cagcccattg atcgcctcgc ccaacgcctc caggtccgcc accatccccc
     4621 cacaccaccg tacacctgtt cgatgcgtca actcaggttt cacgagaggt gtatccgacg
     4681 gttgacgacg ggctctctcg gaacgaccga gaggagctga cgtgctcgac gcagatctgg
     4741 tgctggaagg tggcggcgtc aaggggatcg ggcttgtcgg ggcgatctcg gtgctggaag
     4801 agcgcgggta tcggttccac cgcgtcggcg gcacgtccgc tggtgcgatc gtcgggtcgc
     4861 tcgtcgctgc gggcatgacg gcgcaacgga tgcaggacgt gatgcgctcc gtcgcctacc
     4921 gccgcttccg cgacccgaac ctcctcgatc tgttcggtcc aggtggccag gctctgtcgg
     4981 tgctggtcca caacgggatc taccgcggcg actacctcaa gagttggctc agtgagcaac
     5041 tcagcgacct cggtgtccgc agtttcgagg acctcgtcct cgttgacccg ggtagctccc
     5101 tcccgcccga acgctcgtac cggttggtcg tgatgacatc gaacctgtcg gacaaccggc
     5161 tgacccgcct gccgtgggat tatcccgggc tgggcgtcac caccgatcac cagctggtcg
     5221 tcgatgctgt gcgcatgtcg atgtcgatcc cgttcttcta cgagccggtc aacttccgtg
     5281 gccgctgctt cgtcgacggc ggcatgctgt ccaacttccc ggtcgacgtg ttcgaccgca
     5341 ccgatggcct tccgcctcga tggccgacgt tcggcatcaa gctgtccggc cgcgcctcgt
     5401 cggactcggc gagggcgccg cgcggcgttg gtggcccact cgggatggtc aaggccatgg
     5461 tcgccacgat gacgagcttc tacgaccgga tgcacctcga cgatcccgga gtgctcgacc
     5521 gcacgatgtt cgtcgattgc ggtcacgtcc agcccacaga cttcgatctc accaagagcg
     5581 atcaggacat gctctacgag aacggccgcc gcgccgccga gtagttcctc gagacgtggg
     5641 acttcgacgc gtacgtgcac aagcatcgcc ccgagacgct ggccgcctga cgcgctcgtc
     5701 tgccgggctc ctcgccgtcc tcgacgatgc gacttctttc gagcgcgacc gcttcgaagg
     5761 gtcaagacga gcgaccgtgc gagactccgc ggccatgcgg tacgccttcg atgactgcga
     5821 gctcgacacc ggggtgttcg agctacggcg cgccggcgtt ccagtcaagg tcgagccgca
     5881 ggtgttcgac gtcctgacct acctcgtcct gcatcgcgac cgcgtggtgt cgaaagacga
     5941 actgctcgag gaggtgtggg gtcatcgctt cgtgtccgag tcgacgctga cgagccgcgt
     6001 caaggcggcc cgccaggccg tcggcgacga cgggaccacg cagcgctgca tccgcacggt
     6061 gcacggtcgc gggtatcgct tcatcgcccc gaccgtcgag acggcggacg acgcgacggt
     6121 cgccaaccgt ctggaccgcg acgcggccgc cgaagcgacc gacgcgagca acgccgctgc
     6181 ggccgacgcc gcgactgtcg ccgacgccgc gactgccgcg gacgccgcga ctgccgccga
     6241 cgccgcggcg ccgatccgct atgcgcagtc cgacggtctg aacatcgcct accagatcac
     6301 cggcggcggt gagctcgatc tcctgctcgt cgccggcttc gtctcccacc tcaagctcga
     6361 ctggaccgag cggcggcacg cccacttcct gcaccgcctc gggtcgttcg cgcggctgat
     6421 ccggttcgac aagcggggca ccgggatgtc tgatcgccca ggctcgctgc cgacgttgga
     6481 gacgcggatg ggcgacgtcg tcgctgtgat ggacgccgct cgatcgccga gcgccgtcct
     6541 cttcggctat tcggagggcg gaccgatgtc gatcctgtac gcggccacgc atcctgggcg
     6601 ggtcgacggc ctggtcatct actcgagcta cgcccgccgg ctctgggcgc ccgactaccc
     6661 gtggggcttc cggccagacg agcgggcgcg gtacgcccag cagatcgaac aggattgggc
     6721 gtgggaggcg gacatgcgtc agatgtgccc gaacgccgac gccgagctgg ccgggtggtg
     6781 gggggagcgg tgccgggctg ccaccagtcc cggcgccgcc cgagcgctca tcgagatgaa
     6841 ctcgctggtc gacgtgcgcg atgtgttgag cgcggtccag gcgccggccc tcatcctgca
     6901 ccggcgcgat gatcgcgacg cgaacgtcga ggagggtcgg tacctcgccg cccacatccc
     6961 gggcgccacg ttcgtcgagt tggcgggcac cgaccacttc gtggctgtcg acccggacca
     7021 gatcctcgac ccggtggagg agttcgtccg tgggctggcg ccgtcggcgc cgaccgagtc
     7081 ctcgttgacc acggtgctcg ccgtccacgt ggaggagggg atcgatctgg cctgggtgcg
     7141 aggcatgctg caggccgagc tcgtacggca tcgagggacc cccgcggcgt cggacgacac
     7201 cgcggtcctc gccaccttcg acggacctgg tcgggcgatc cgttgcgcgc tggccctcac
     7261 ccagcaggct gcgcgcgccg ggctgcacct ctcggccggc ttgcacaccg ccgagctcgc
     7321 tcggcgtggg acgcaggtat ggggtgacgg cacatcggtc gcgcaggcgg tcgccgatca
     7381 ggcgacagcg ggcgaggtgt gggtgacggc gacggtccgc gacctcaccg cgggctcagg
     7441 gttgtcgttc gagcgacggg agaccctggg gttggcatcg ctcgggcgtc agctcgagct
     7501 cgacgcagtc cgctgaacgg aatcaggcca gccggcgacg ccgccgcttg aagccgaccg
     7561 cggcgagcac ggcgccgaca aagaggagct gccagccgag ccggacgatg cgcatcactt
     7621 ccgaaccggt gggcggcaag gtggccgcac cgacgacgtt tgcgaagctg tggttgttgt
     7681 ccgggttcgg atcgaccgtc ttcgcatcga ccgaggccag gttggtgaag gtaccgaccg
     7741 ccgtcacccg taccgggacg acgatcttcg tgctcgcccc cggtgcgagg tcgccgaccg
     7801 cacatgtcag gaccgacccc ttcagcacac acccggccgg cacttgcaac accgtgacgc
     7861 cagccggaat gagatcggtc accacgacgt cctcggccgt gtcgggaccg aggttcttca
     7921 ccagcaacgt gaacgtgacc tcgtcaccgg gcgtcaccat gccggaactc acggacttgg
     7981 tgatcacgac gtcagccttg ggcacgcgcg tgttggtgaa ggtgcacacg accgtctcgc
     8041 cggccgcgag attgatgttc gccgtggcgt gagcggtgtc gacgacggtg ccgccgctcg
     8101 gatccgtgca ggccaagccg gtcaacgcgt agccgctggt gggcccttcc accgccttgt
     8161 agggaccggg agccagcggc gtgaacgtac gcgatgcgcc ggacgccagg gtgaactggg
     8221 tcgtacccaa cgcgaacggg aaggtcgcgg tgctgggcgc ggtggtcttc ttgacgatcg
     8281 tgatcgttcc cggggcaacg acggtgactg tcgcgttcgc ggtgtcggtg gcatcgcaag
     8341 gatccggatt cggcgtctgc gctccgcaca gtggagcacc caccatgtcg gtcggcgtgc
     8401 cagtgaccgt cacggtgttg gtcgtcgtct tgtcgagcgt gaccttgcac gtgaaggtcc
     8461 agatctcgtt ggcggcatcc tcgaagatgc tgttcggcgt gtcgagcaag ccgtccccgt
     8521 cggtgtcgcc cgagacgtag gtgagcggcg agcaggtgtt gtcggtgatg cgggtcttga
     8581 cgtcgcccag tggcacattc ccggtgttgc gcacggtgta cgtgtaggtc acgacaccgc
     8641 taccgaggag cagcgtgggc gacgcggtct tggtcacgtc gatcgccggg gtgaaggggc
     8701 gcgtgaacgc gaagtcggct gcgaacacgt cgatcgggac ctgcgggttg aagccgagtc
     8761 ccgccgtgcc cttgacgatc gcgatgtcgt gggtcggctg cgtgatcgtt gccgtgcact
     8821 cgtagtgcca gacctcggcc ggtacccggt cgagctggtt gtttccgttc gtgtcgccgc
     8881 ccacgaaggt cggcgatgcg caggcgggag tggccggtgt ggacgaatcg gcgagcgtca
     8941 cgttggccag cacatcgtcg gctgggaggg ggctcgtacc gatgttggtg gcatcgaagc
     9001 cgtacgtcac ctgccgaccg ctcggcacca actccgagct caccgtcttg acgaggtcga
     9061 tggccggatc gagcacgcga accgaggccg tggccgtgga ggtgtacagg ttgcccagac
     9121 catcgacgcc gccgaccgtg accgtgttgt cgttcgtcgt cgccggtggc gtgggcaggc
     9181 cgacctcacg ggtgcacgtg taggtccacg actcgggcgc cccggagtcc gccccatcca
     9241 ggaacccgtt gtggttggcg tcgttcccag ccgccggcac gagcggcgag cactggtcgt
     9301 cgttcgggcc gaggacgttc aggccgacgt cacctgtgtt cgagacgacg aacgtgtagg
     9361 tcacggcgcc gccgacttcc acgaccgggg gcgacgcgct cttcgtgatg tggatgctcg
     9421 gctcggtgac gagcacctgc gcctggtcgg acgcgctcac cgtggcgtcg gggtgcaggg
     9481 tcccgctgac ggacggggtg ccggtgaccg agacgtggtt cgtgacgaac gccgactcgg
     9541 cacccgtggg cggtggggtg ccctgcgagc gctggaggag ggtgttgcag ctgtagtccc
     9601 aggtctcgcc gacgtcgagc aggccgtcgg cgttcgtgtc accaccctca aaggtgagcg
     9661 ggccgcagat gacggggttc tcgactggcg ggttcgggtt caacgccagt ggtacgtcac
     9721 cagtgttcgt cacctcgtag ctgtactggg ccggacgtcg aggctggtcg ggtccgacga
     9781 ccacaggcgc ggccggatcg accaccaccg gcacgagcgc gagcttgttg atcgcgatgc
     9841 ccgggcgcag gacgtcgacg aaagcctcag cgtcgtcgtt gaccgtcggg acgccgggta
     9901 gcggcgcgcc cgtcgcgtcg gatggctggc cgacgatgtt ggccacgttc tcggtaggcg
     9961 tgttgacaag gccggggcag gtgaaggtcc agctctcccc gggatcgagc aaggagttgt
    10021 tgttcgtgtc gccgctcacg aacgtcgcgg gtgaggtgca atgcgggtcg gtgacccagc
    10081 cgggatcctt cgccggtcca gccgtcgtcg cgccgggacg gttcaactcc ggcgtgcccg
    10141 tgttggtggc gacgaacgtg tacgtgaccg gctgggcggt gccggctggc ggcaggacga
    10201 caacggtcgg gtcagccgac ttggtcagcg tgatgccggg gtggaccacc tcgaccgcgg
    10261 cccgatcgat ggagacgacc ggcgggttcg ggttgggaaa tggcgcgttc gcgttgttcg
    10321 ggttcagcgg cacgccggta acagtggcgg tgttggtgac cgccgcggtg ggcgcggccg
    10381 agcacgagaa gtgccaaatc tcaccgacat cgagcgtggt gtcgttgttg cccggtccgt
    10441 cggcgccgcg ggtgggcgcc tggcacggtg gcgtgttgtc gacgagggcc acgctgccga
    10501 gcggcgtatt gccggcgttc gcgaccgcgt aggtgtaggt caccgtcgta cccgacgcca
    10561 cggtcacgac cggctggccg ttgaccaact tcgtcaactc gatggcgggc gtgaagaccg
    10621 cgacatcatc ctgggcctgc gcggtgacgt tcgtgccggt cgggtcagtg ccgcgggcgg
    10681 tcgcggtgtt cacgatgaca ggtgccgccg atcgcaggcg agactgaagg accggccggg
    10741 tgcacgtgaa ctgccaggac tcggtggtat ccagcttgcc gtcgtggttc gtgtcgccgg
    10801 cgttgaaccc cgtcggcggc accggtgagg cggcgccaca gaggtcatcc acgacggtga
    10861 cgttgctcag tggagtgttc ccagggttgg tgacgacgta gttcgaggtc acggtcgccg
    10921 gcgggttgag gtcaccgcgg acctcgctcg gcgtgttcac cttggtcacg gcgatcgcgg
    10981 gttgatagtt gaacgagttc cacaccgtgc aggtgacgat ctcgtcggct atggggttca
    11041 gcgtgaacgc gcccgtggcg tcgagctcgc cctcgacgac ccgaacattg ccgtcgacat
    11101 ctttgagctc acatcggaag tcggacgcgt tgggacgccc ggcgacgaag cccggtcgca
    11161 gcgtttcccg gacggtggcc tgcgagtgca atgttgatgg caccggttcc cactggaact
    11221 gggcgaagcc gttggcgtcg gtgacgagcg tcttggacgt cgcaggtgtg gtgtcgggca
    11281 ggatccagtt gaacgtgccg cccgggaccg tcggtgtcgt ctgcatcgtc cagccgggcg
    11341 cggggacgta gtcggcgctg ttggccgact gggcgagctt gcggatcgtc agcgacggtg
    11401 agcacagctg cagcacgacc gtccggagga acttggccag gttggcgaag tcgtggacgg
    11461 tcgcgacgtc gacctggttg aggctggtga tgctcgccag ttcgctgtcg tcgacgacct
    11521 gtggtcccga aacggcgacg agccgggcga cgctggccgg ggacgacaac gcggtgccga
    11581 cgccgaccgc gagcatgcga gtgccggccg ttttgatctg gtcggcttcg tccaccgcac
    11641 gggcgagcgt cgtggcgtcg gcctggccgc ggttggtgtt gtaggcgacg tgcggcgcag
    11701 cgaacggatc gccgggctgg ccgaagtcgt aggccgtcgg atcaccgtcg gtgacgaaga
    11761 ccaccagctt cggcagcgtg ccggtaccgg cctggtcgag ggcctggtcc cagttcgtgt
    11821 actgggtgtt cgcggtcggg ttggccgtgc cactgttgga gagcgtgaag ctgctggcac
    11881 tggtgatcga cccggtgccc cggtactggt agaagttcac gttcgccggc cgagtcggga
    11941 tcgggttgta gtagccggcc aaggccctgg tcagcacgcc gttctggccg agcgaggtgt
    12001 cgtcgacgag tgtggtgggc gcaagctgcg cgcccaccgt cccgaactgc gtgacccgcg
    12061 ccgcggagct cgtgttcttc agcgcgtcga ggaatgcggc agccgcacca cggacgttct
    12121 cgacggcgcc ggaggactga atggagcccg acgcgtcgag cacgagcgtc acgttcacgc
    12181 cacaggagtc ggcgatcggc gggttggtga cggctgcagc ggccggtggg gtcgagggaa
    12241 ccgaagccgc cgcccacgcc agcgggacca tcgtcatcgc cgcggcgatg tgggccacac
    12301 atcggccccg agccgaacgc attgcggcat tcttcctgct cagtgcttgt ggatacaagc
    12361 atcgccgtgg tcgagacggc gctgtgggtg tctcggcgat ggccggggag ttgccctgac
    12421 ggcttggaaa ccctctgcta ctccgtgggt tcaggcgccg agcacttctt ggtaggcggc
    12481 ctccgtctgt gctgacgggg tgacgccgag gtcgcgagag agcgtggagc ggcactgctc
    12541 gtacgcgcgc agggcgtccg cgcggctgcc ccgttgcatg tgcaagcgca tgagccggac
    12601 atacatggtc tcccgatacg ggtccagcgc gagcgcctcg ttcgcgtagc ggaccgcgtc
    12661 gtgctcgaac ggaccacccg tcaggccctg caccagcacg tcgagcgcgc gaagcatcgt
    12721 tgcctgcagc tcgtcgcgcc ggcgttcgac ccacagtccg tgttccgccg gtaggaatgg
    12781 cctccgggcc aggctggcgg ccgtcatcgc cgcgggcacg gcggcgtccc ggttgccgag
    12841 tcgccaggcc gcctccgcat cctcgacgaa ccggaccgcg tcctgcacat cgacggtcga
    12901 gtccggaggg aggcggatct cataggaacc tgaggcgctc tgcagcgcgg agccgaggcc
    12961 gatggattcg agggcggcac gcagcttgga cacgatcgcg gacaggtctc gctcccagga
    13021 cgcggtcagc gcgtcgggcc acaccaggtc agcaagctcg tcccgtgcga cggcgtgatg
    13081 gtgcaacgtg aggtacgcca gcacgatccg gccctgccgg ccggggagcg ccgcgccatc
    13141 gagccaacgg tccccgcgtt cgaccccgag gcgacccagc agccggaccc ggaccgtgcc
    13201 agacaagcgc cagattcctt ccaaccgtcc tgcaaccgcc gattcgtagt gtgccaccga
    13261 tcgaggagaa gccatgacga aggtcatcaa ctgcgactgc ggacgcgtgc tgcgggcggc
    13321 aacggacgac gagctcgtcg tcgccgtcca gcagcacgcg cgcgaagcac acggcatgga
    13381 gctcacccgc gaccaggtgt tgtcgctggc tgtgcccgag tgaccagtcc gacgaaccga
    13441 ggaggaacaa tgaccgttca ctaccgatcc gcagccgtcg ccggcgtgca ggtcgcctat
    13501 cgcgaagccg gcaacgccgc caacccccct ctgttgctcc tgcacgggtt cccggcatcg
    13561 tcgttcagct accgccacct gatgaccgca ctcgccgacc ggttccatct gatcgctccg
    13621 gactatccgg gcttcggcaa cagtccggcg cccaaccccg acgcatggcc gtacacgttc
    13681 gatcacctcg ccgacaccgt ggaggggctc gtcgaccatc tcgggctctc gcacttcggc
    13741 ctctacatgc acgactacgg agcgccggtc gggttccggc tggcgctccg gcgtccgatc
    13801 gccgtcgact tcctcgtgat ccagaacggc aacgcctacg aggagggctt cactggcgta
    13861 tgggacggga tgcgcaacgc gctctggcag aaccgctcgc ctgagaccga gaagccgctg
    13921 cacgccttct tcgagcccga cggcatcaag tggatctaca ccggcggcac gcgcgacccg
    13981 gagcgcatca gccccgacaa ctggaacctc gatcttgccg tcttggcgaa gcccaacgct
    14041 cggcggatca acctcgacct gttctacgac taccgcacca acgctgagct ctacccggtc
    14101 tggcaggagt acctacgacg tcacgagcca gcgaccctga tcgtgtgggg ggcgaacgat
    14161 cccatcttca ggtccgaggg ggccgaggcc tacttgcggg acgtacccca cgccgagctg
    14221 cacttcctcg acagcggcca cttcgccctc gaagaccacg gggaggagat cgccgggttg
    14281 atcagggcgt tccacaccac tcgagtcgcc cgggccgtct ccagcgccgc gtgaggcgcc
    14341 ggccagccgg tcagccgaag atggtcctgg tgccggtgta gatggtcagc agcagagcga
    14401 gggtgcccac gaggtcggcg gtcatcaccc ggcgggtcgt catgccgacg gcaccggcgg
    14461 ccgacaggac gttgccggtc gggcgcagga cgacgccgac gaccccgagc ttgaccatca
    14521 gccggcgggc cttggccggc acgaagcgcg agccgtaacg gcgaccgagc agcagatgga
    14581 tcgggtcccc gatcaacatg cggggcacgg cgatggcgaa gaaggccagc ggtgaaacgt
    14641 ggctcttgac cgcgtagacg aggatcggga gccgaggcgt gaacgccatc atcaggagcg
    14701 ggaagtggat cagcgccggc gtgatcgccg tgccgatcgc cgacaccgct gacagcgccg
    14761 cccagccggc gagcagccag cgggtcttga tcttggtgcc cgggacgtcg gccagcccat
    14821 cggcggtcgg ggcgaggcgg gagcgcactg cggtgacgaa gcggcgcacc gacggcggaa
    14881 cggtcagccg gctggtcggc cgcatgacgg tgcgaaccgt cgcccgagtg gttccgaccg
    14941 cagttgcgac aacggtcgct ccaacggtcc gctcgatgga tgcaatgcct gcatcggtgc
    15001 gcgccacggc gatcgtgttc atcccggtct ccctccgtcg ggcgactcac cgtcgcccct
    15061 tcccacatcg cacggtaagg aagcagcgct tcgggcggaa cgctgctagc caccgagttc
    15121 gacggccgcg accacgtcgt cgcgatgcgg aaaacctcag aggctggcgg aggtcgacag
    15181 gaagacgcga ccgtcggcga cggtcacacg ctgggacgat ccggcgagcg cgtatctgcg
    15241 actctgcccg agctcggtga cgtcgacgct ccacctctcg gcgccgtcgg cggcatcgag
    15301 gtcgacgaca cggggtccgc ccgccaccac gacaccacct ccgtaggcgc cgagcgcagg
    15361 tagaccggag gcgggagctt cgtcggatcg cagcgagctc ctccaccgca gcgcaccagt
    15421 gtgagcatcc agcgcgacga ccgttcccga ttggtctccg cccgcgaccg gcgaggccgt
    15481 gtacacgtag atcatgtcgt cggcgccggc ggtggcgcgg atcctctcgc cagcgaagct
    15541 gatctcacgg accgtctgcc cggtatctgc atcgagcacg acgagcgggc caccggtccc
    15601 gctggcgacc gcgatgcggc tgtcgaggac gaccgggagt tgcgcggcgc gaacggacac
    15661 ccgccagcgc tcagcgccgg tccgagcgtc gacagcgcgc acgaagtcct tggcccggtc
    15721 gacgaggaag acggtcgagt cgtcggctcc gatccggtag cccgccgtcg ccccctggtc
    15781 ctggcactgc cggtcggtcg tcgcgtaggc cgacgtcccg tcggcgacga gtaccggccc
    15841 tcgatcgacc ggaacgcacg cggaggatcc ggcgcccgtg cccccggtcg tcgcgtcgac
    15901 gatgacgccg ccgttcgccg tggcggcaac gagcgttgcg tcgcggggga gggctcggga
    15961 acgggagtgg ccaggacgca gccggtcgcg ccaccgttcc ttgcccgacg ccacgtcgag
    16021 ggcgacgagg gcccgatcgc ccggatcagt cacgtacagg tggccggcga tgaccatcgg
    16081 gctctcaccg gcgcgcgcgt tcacccactt cacggcgcct gtgcgagcgt cgagggcgta
    16141 gagccgggcc cgcggcgagg cctcgaggaa cggcccgatg gcgatgagcg cagccaccgc
    16201 agcccaggcc aggccaccga gcgccgcacc ccgcagccga tccggcccga gcgcgaacga
    16261 cgcgaccgcc gccgcgctga gcacgagggc cgcgccggcg acgggcagcc ggagcgtcgc
    16321 gtacaggtgc cagtctccgt atgacggttg gtggacgaga aggatcgcca cgaggagcgc
    16381 gggcacgagc gcgagcagtc cgaggagcag cgtccgcttc ccggtaccgc ctccgcccgc
    16441 agtcagcatc ggccctcacc cccggcggaa cgttacgacg cccgagcgcc gggcgatacg
    16501 tctctgggca ctcggccagg ggaggctcga ccgacgaggc ccgctcggcg ggtgcggaaa
    16561 accgcacccc ccgtacgcag ccggcaccct ggaccgatgg gggaccactg acgatgatgg
    16621 aggcgtgacg aagtccgcag cgctcgcccg cgccgtcgtg gcggtggcga tcgctacgac
    16681 cgtggccgcg tgcgtcctcg cggtcggcca gtcagggccg accggcgtcg acgcgtggag
    16741 cggcggactg gccggcttcc ggctgtggac gtttgtcgcg ctggtgcccg tcgtcgtctt
    16801 gaccgtcgtc gtgcacctgc accgacccga ccacgcgctc gggcggctcc tcgccgtcgc
    16861 gtctgcgctc ctcggtctct cgacgtgcgc cgggatctgg ttgcggacca ccgatgtgca
    16921 gacgctgcgc gatggggtag ccatcgccgg cgcggtcttc gacatcgcgg cgatcgtcgc
    16981 cgtcggtgcg ctcctggcgc tgttcccctc ggggcgcggc cctcgtccgt tcgagcggtt
    17041 gctgctctgg gcggcaggtg gattctgcgt cgcggtcgct ctgctgctgc tcggcgcgcc
    17101 cctgcggagc gtcgcaacgg caacgccacc atggtcgccg ctgcaggtgc cgtcgctcct
    17161 gttcttcggt ggccgcggtc aggggttcct ggcctttgcc gcgctcctgc cgctcgtggg
    17221 catcggcctg ctcacctggc gctcgccggc gatggacggc gaggagcgca tccatctgcg
    17281 ttggctggcg ctggccggcg tgaccgcggc atcggtgctc gcgctcgtcc acctcgtccg
    17341 ccggctcggg ctgctacagc tcggcggcga cttctacggc gttgcgccgc gagtcatcgc
    17401 gtggttgctc gtcgccgcgg cggtcaccgt caccgcgctc cgtccggacg tggccgacgc
    17461 ggctgccgcg gttcggcgcg tggcggtggc cgcggcgctg gtcgtcaccc tcggcctcgc
    17521 cggcggcctg gcctggtacg cgaccggtgt cagcaccggc acgtggctgc ccggcgcgcc
    17581 cgcgctcgcg gctggcctga cggtggccgc gctgttcttc ccggcccgcg aagcgttcga
    17641 acgtgcggct gaccgatggc tgttcggcga cgccatcggc gatgcccagc tgctgagcgc
    17701 cttcggtcag gcggctgagc gcacggccgg cctcgacggg ctggccgagc tcatcgccac
    17761 cacggctcgg cgcggtctgc acgtgatgtg ggcgacggtc tcgttgcacg acccggagcg
    17821 cacggagaac gccccgaccg ccgaggcggg gctggtgccc gatgcccctg tcccggccgc
    17881 atccgtcgag ctggccatcg gcggcgatgt cgtcggccgc ttggactgtg ggcccaagcg
    17941 ccgcgggagc ctcaccgatc atgaccggca gctgctcgcc gcgctcgccc gcgaggctgc
    18001 gctggacctc cagaacgccc ggcaggccag cgcgctggcc gagcaactcg tccagatccg
    18061 tcgccaggcc gaggagctca ccgcgtcccg gactcgcatc gtgcaggccc aggaggccga
    18121 gcgccgccgg atcgagagga acatccatga tggcgcgcag caggagctgg ccgcgttgct
    18181 cgccaagctg cggctggcgc agaaccagct cggccgcgat cccgacaccg ccgagcgcac
    18241 catcgaagag gctcagcacg acgtccggcg agcgctggac gacgtgcgat cgctcgcccg
    18301 tggcatccat ccggcagtct tgagtgaccg cggcctgctc gaagccatcg agagcagcct
    18361 cacccgcttg ccgctggagg ccatcatcga agccgatccg gctctgcgcg cccggcgctt
    18421 ccccgacgag atcgaaggcg cggcgtactt catgtgctcg gaagcattgg cgaatgtgct
    18481 gaagtacgcc tcggcgacac gggtggaggt ggtgctcgca gtgcacgacg agcggctctg
    18541 gctgcgggtg acggacaacg gcatcggctt cgatccacaa cgcgtcgacg gcaccggcct
    18601 gccgaacctc gctgaccggg tcgaagcgct cggaggccgc ctacgggtgc agagcacgtc
    18661 aggcgccggg acgaccgtcg ccctcgaact gccagtggtg ctgcggcagc cggcgtcaat
    18721 cgtcgagccg gcaaccgtcg gtcatccagg catggacggt cagccagcga tgagcgactg
    18781 gccggcgtga acgcgccgat ccgactggtc atcgccgagg acaacttcct cttccgcgaa
    18841 gggacgcgcc ggctgctcga ggactcgggc gagctggagg tggtggccgc ggtcggcgac
    18901 gccgatgagc tcttgcaggc ggtggatgcg ctcgagcctg acgtcgtgat caccgacatc
    18961 cggatgccgc ccggtcacca catggaaggc atcgccgccg cccataccat ccgcgaggtt
    19021 cacccgagca tcggcgtggt cgtcctgtcg cagcatgccg aggagggcta cgcgttcgag
    19081 ctgctgcgcg acggcaccgc cgggctcggg tacttgctga aggaacgagt cggtgacctc
    19141 gacgagctgt tccgagccat ccgcgaggtc atggcgggtc gatcggtcat cgacccgaag
    19201 gtcgtcgagg gcttggtggc ccggcaggcc cgcgtgtcga cgtccgccgt gcacaagctc
    19261 acgccacgcg agcgcgacgt gctcggcgag atggcgcagg gcaagacgaa cgcggcgatc
    19321 ggcgagtcgc tccacctgtc gttgtccgca gtggagaagc acgtcaacgc catcttcatg
    19381 acgctcgggc tgaccgagga ggcgcaggtg aacaagcggg tggcggcggt gctgctcttc
    19441 ctgcgcgaca gcggcctgac gcccgcctga ccgcgcgctc ggtgcctcat ggctccggcg
    19501 tctcgatcgt cggctgagcc tcgacctcgt catcagtcgt cctcaggcgg atggcgtcga
    19561 tgagggtcgg ggcccggacc ggggcgccga acgacggctt ggctcccgga acgaggaccg
    19621 ccgggtcgga ccctcgcgac agtcgcccca gaaagccacg atcctcggcc aagactggcg
    19681 ccgcgcacgc gccgatgacg acgatgatcg cgctgcccga gaaccaggtg acgagcgaga
    19741 gggcgacccc gaagaagccg aactgggcct ggttctggct catcgtccgc ggcatccaga
    19801 ggaacgccgt cgcaccgtag agcgacaagg cgacgccagt gacgagaccg gtggggaaga
    19861 ggacacgcag gcgcaccttg cggctcagca tgaaccacgg cgtgatccac cagagcgcga
    19921 tcgagccagc cagcgcgagc agcgcgaaca cggccagctc gggcccgtga ccgagcagga
    19981 cgcgcatgcc gccgatcatc gacaggtagg caaagatgcc ggccagccag accgcaccga
    20041 gggcgtagct cgacaccttg ctggcgggcg gccgccgcca agcgcggaca tagacccgct
    20101 gcagggcgga cgtgaacgag ctgacgtaga agaacgtgaa gatcaggccg agcacgccgg
    20161 tggcgcgccg gatgctgtca gacgtgccga aggcggcgtt gaatgtcgag aggccgtcgc
    20221 cggtcaatcc gccgtggcga acgatggcct ggccgatcga cgttcggatc gaaggtggcg
    20281 cgaacgacgc cagcacgatg atcgcgggaa agaaggagac gaatgccttc gccccgaggg
    20341 cgacgctgcg atcgacgaac tcgttctcga gcatccgctc ccacacgcgc cagacgatgg
    20401 tgcgctgggc ccagtcccgt ccggcgtcgg cgagcttgac gaccccgtcg gcgcgggcgc
    20461 gtacgttctt caggtactgc ggaggacgca tcgggccatc atgtctccgc ggcgccccgg
    20521 gtgcgttcga tgctcgacga cggcgccgat ggtcctacgg tccttcgcat gatcgagcga
    20581 atgagcgacg tatcagaggg catcatcggc ttccgattca gtggccaggt cacgaaggcc
    20641 gagtacaccg agaccgtcct gccggccctg caggcggcga tcaagggcga cgggcgcgcc
    20701 cgcaccctgg tcgtcatcga cgaggggttc gagaagttcg agccgggtgc cttgtgggag
    20761 gacgtgaagt tcggactcgg ctccgggatc acccacctgt cgaagtggga acggacggcg
    20821 ctggtctccg acaaggactg ggcccgtcac gccatcacgc tgctcggctg gatggtgccg
    20881 ggtgacgtga agatcttccc gctggcccaa ctcgaggacg cgaaggcctg ggtggccggc
    20941 tgaccgcggc gcggtcgtcg tgcggtgatc gcggcgccgc acgagccgcg agtgtcaaac
    21001 tgtccgccgc cgtcgagggg gacgatcgtg ggcagaccgc cggatgaccg gttccggatc
    21061 ttcatcagcc acaagcacga ggaccacgcg ttggccctgg ccacgcacca ggcgctcgag
    21121 agcctgaccc cgacggtgga gtgcttcgtc tcgggtgtcg acatcgtcgc cggctccgac
    21181 tggaaccgcg agatccggac ccagctggcg cagagccacc tgctcgtcct tctcttcacc
    21241 caacccacgc agacgtggga ctggtgcctg tacgagaccg gtctgttcac ccggttcgag
    21301 gccgatgagg tcaccgccgt ggtgtccatc ttcagcgccg actcgggcgc gccgcgggtg
    21361 ctcgccaacc tccagggcgt cgaggcccgc cacgaagcgg tgaaccgctt cttgaccgcg
    21421 ttgtgcaaag agacatggcg ggtgtcggac gactggcggc tcggcgcgct ggcgccgcgc
    21481 gtcaagccgg cccagatcga gcgcgccgcg gccaagatcg tcaacgcctt cccgcacggg
    21541 tcgcgtgacg acagcccgta ctacccgtgc catcgtgtcg tgctcgacct gcgcgccatc
    21601 gacgagatcg gcgaccacat ccccgaggaa gctcgggtga tcgagggtcc caacgacacg
    21661 acggggttca cgctgtccct gttcaacatg gctgacggcc gacgccatct gacgtggcgg
    21721 gacctgctgg tcgcggtgga cggcctggag gccggctggc gcgcgcagct cgaccgtcgc
    21781 ttcgtctgcg cgctcaacga gcagctgttc acgccgatca cggcgacgct cagggcctgg
    21841 aaccagggcc gccgccacca acggctcctg aagccggtcc tgtaccggat cctgcgggcg
    21901 ccggtgccgg cgggatcacc tccggacgcc agaggccggc cgacgggtgt gacggtcgtg
    21961 ttcgaccccc agctggcgcc gacccaggtg ggcggccccg cgctcaacct cgtgcgcatc
    22021 aacgcccggt tcggcgtcga ggtgttcgac gagttcgtcg gcacggtccg ccatcgagtt
    22081 gccgacggcc tcgacgtgtt cgtcgacatc ggcgaggcca tacggctcgt ctacgaagag
    22141 gccgaccgct tcggagtgtt cgacgagaac tcccttcgcc gtgcctacgg cccggactac
    22201 gagtcgaagg gcatcgaggc gatgggccgt gaatggatcg acagcctcga gcggctcaag
    22261 aagggtttgg aggagcgcga cgccgacctc gtcgaggaac agctcgctgc gctgcgccag
    22321 ctcaaccggt cgttctccac cgcgtcaacc gagcgctacc tggacctcct gcgcgccggc
    22381 gcgtcctagt gcgagggcgt cacttgtagc gttcacccga agattcatgg caaagcgacg
    22441 tctgagcgtc atctggaccg gaatcttcgg gtgaaagcga catcgaaagc gaggcagcta
    22501 tgagtgctcg agtggtgcac ttcgaggtcc cgttcgacga cggcgacagg gcccgcagct
    22561 tctaccgcga ggtcttcgac tggcagatcc aagccgtgcc agagatcgga tacaacatgg
    22621 tgtccaccgg cccgacgagc gatcagggca tgccggcgga gccgggcttc atcgggggcg
    22681 gcatgttgca acggcagaca ccggtcaacg caccggtgat caccttgcag gtcgacgaca
    22741 tcgacgccac cctcgtcgcg gtcgagaagc acggcggcgc caaggtcggc gacaagatgc
    22801 ccgtcggtga catgggcttc gcggcctact tcaacgacag cgagggcaac ctcatggggt
    22861 tgtggcagaa cgcgggttag tcaactcgtc tccccccgag ctgaccgctc gggcatgagg
    22921 aaatcggtcg ctcgcccgag cgacagctcg agcaccgacc gggtgatctc cgagtacgtc
    22981 gggtgggcgt ggatgatgtc ggccaccgcg tgcaccggcg tgccgagggc gatcatgtcg
    23041 gtgagctcct gcatcagctc ggacacgtag tagccgatgg cttgggcggc gacgaccttg
    23101 ccgtcgcgca cccagatctt gacgaagccc tcttcgtagg actcgatgcg cgcccgcagg
    23161 ttggccgaga acggcacctt gaacgcagtg accgcgtcgt cgctgccgtc cggcacctcg
    23221 ccgacggcca cgacctcggg gatgctgtac accaccgagg ggatcacgcc gtactccatg
    23281 ctccgacgcg ggcggcccgg ctccgtcatg atgttgtcgg cgcagacgag tgcctgctgc
    23341 atcccgacgt gagccaggat cgacttcccc gtggcgtcgc cgatggccca gatgttggcg
    23401 acgctcgtgc ggagttgcgc gtcgacaccg atgcccttcg tgctgtgctc gacgcccagg
    23461 tgatcgaacg atggggcatc cacgatcggc tcccggccga tggcgaccag gacggcccgt
    23521 ccagggatgt ccacgatgtc gccggtcgcg ccgtgggcgg cccgcacata gccctcttcg
    23581 accgagatca cgcggtgccc ggtacgagtg cgcacgccga tcaggttgag ctcctcggtg
    23641 agggcgtggg agatctcggg gtcgagcgtg ccgagcagcc ggtcctgcag ctcgacgacg
    23701 gtcacgcgcg tccccatgtt ggcgaagatg gtcgcgaact cgaggccgat cacgccgccg
    23761 ccgaccaccg tcaagctctc cggtagctcg ccgatcgtga tcagccggtt gctgctgacg
    23821 atgtacggat ggtcgggcgg agcccacgac ggcatcaacg gccgcgagcc cgtggccacg
    23881 acgatgtgct cggcctcgat catcgtctct gtgcccgtgt acgacatctc gagcccgtcc
    23941 aggccgagct tgccctgccg gatgcgcagg cgtccgggcg acacgatctg cgcctcgccc
    24001 tggaagacct gcaccccgtg gtgctcgagc aggcggctga cgatgccccg cgagcggaga
    24061 atgacctcgt cacgtcgggc cgccaccttc tcgaagtcca cccagaagct cgacacgttc
    24121 acgccgtagc ggccggcttc ccgcacgttg cgggcgatct tggccgactc gatcatcgac
    24181 ttgctgggcg tgcagcccca attggtgcag atgcccccga cgaagtcacg ttcgaccacg
    24241 gcgacacgcg cgccgagctg cgccaagcgc accgcgcacg tgtgaccggc cggcccggcg
    24301 ccgatgacga cggccgtgta gtcgctcaac ccgatctcct ctggctcgtg gaccgcagca
    24361 tgctgccagc ccgacgcgtg cgcgacggcg atcccgcgcc gttcggggcc cgctcaggcc
    24421 agcgcgccct gcctccggtg gcccagcaac accaccgcgc cgatcgcgct cgcggtcatg
    24481 acccccgccg ccatcccgag cgccgtcggt acaccggccg tttgcgcgac gatgccgccc
    24541 accgcggcac cgaagggctg gccgccccac gccaccatcc gtccgacgac gttgacgctc
    24601 gatcggagct cgtcggggct ggccatctgg cgataggtga tgcccaccga gatcgtcgtg
    24661 ctcaccgcga aggcgaacgc gatcgtcagc acgagcgcga gggcgtagct cgtgttggcc
    24721 gtcagcagca ccagcagcgc cgccgacaca ccgaggcaga gtggggtcag ccagcggatc
    24781 cggtcgcggg cgtagatccg ggcgaaggtc agaccggcga gcagcgcacc gagggcgtcg
    24841 gcggtgaaca gcaggccgag ccgcacgctg ttgtccggca cgccgagacg atcgaccgcg
    24901 tacggcacga ccagaccgag catgacgccg aaggcgaagg agttcccgaa gccgatgacg
    24961 atgagcgtca ggatggtgcg ctcctggcgg atgaagcgca gcccccggcg cacctgttcg
    25021 ccgaggccgg ccgagcgcgg ctcgggcacg cggtgcaggc tgaacggggc ccggatcgcg
    25081 ccgaggacgg cggcggcgat gaagaagctg gcgacgtcga accacaccgc gctcgccgcg
    25141 ccgatggtgg tggcgagcag gccgccgagg gcgggtccga tcatgagcgc cgcggactgg
    25201 atcgaggtga gcaggccgtt cgccgcggga agtcgctggt caccgatcag cgcggggagc
    25261 gcgccgaaca ccgcggcatc ggagaagacg aacgctgtcg cggaggcgaa cgaggcggcg
    25321 tagacgacg
//
LOCUS       DATBFZ010000391         8025 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_5061848, whole genome shotgun sequence.
ACCESSION   DATBFZ010000391 DATBFZ010000000
VERSION     DATBFZ010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 8025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8025
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5061848"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="VH419_11800"
     CDS             complement(<1..285)
                     /locus_tag="VH419_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012922571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY7044343.1"
                     /translation="MTLLRLDAVRKHFGPHHVLRGIDLDVEEQQAVCLIGASGSGKST
                     LLRCVNLLETVDDGRIWLDDRDITDPRVDPDDVRRDVGIVFQAYNLFPHMS"
     gene            complement(285..1160)
                     /locus_tag="VH419_11805"
     CDS             complement(285..1160)
                     /locus_tag="VH419_11805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012922572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEY7044344.1"
                     /translation="MSDVVRTTGHAEEWRPSTLQEGRLEYRRRRALRRGATAAVSTVV
                     VLALLGILVVTAPGWERVQQTYFDVGYARDVFPELARAFVLNIKLFLVAEVFILVVAL
                     VVAIIRVVPSPAMAPLKLLAIIYTDVFRGTPTLLVIFLVCFGIPALDLQGTPTSIFWL
                     GVIALTLSYGAYVAEVVRAGIASVHPSQWAAARSLGLSYQRSLRHVVLPQAIRRVMPP
                     LLNDFVSLQKDTALVSTAGLVEILRQAQIDSSRDFNFTAYVVAALFFVVVTVPLARLA
                     DWLALRQLRREQGAA"
     gene            complement(1164..2060)
                     /locus_tag="VH419_11810"
     CDS             complement(1164..2060)
                     /locus_tag="VH419_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008356025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY7044345.1"
                     /translation="MRQRALSPAFVVGVSVLCLLSVGCAPNDDNNDSGGATTSPETSS
                     GASTTDACADPQTFKPGVLTIATDDPAFEPWMVDNDPTNKKGFESAVAYAVAEKMGFS
                     DDQVEWTRVPFNSSYQPGPKKFDFDINQISISAQRAKAVTFSDGYYTADQAIITMKDS
                     PYADATSLADFKDATLGAQVGTTSLSAITDTIQTTAQPQVFDDTNLAKNALQNGQVDA
                     IVADLPTAFYITAAQIPGSTIVGQFQSTVGQPEEFGLLFEKGNPLVECADQALSTLKD
                     KGTLAKFQQRWLSQSADVPTLS"
     gene            2140..2967
                     /locus_tag="VH419_11815"
     CDS             2140..2967
                     /locus_tag="VH419_11815"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEY7044346.1"
                     /translation="MHIVTVGDVVLDILVNAPRGLRTDDDTEARIALAAGGQAANVAT
                     WSAALGARATVIGPKGQDIAAGYIESGLARRGVDMIPIDVSATGKVVAIVTGRERTLA
                     SDAGDQSWAARLDPNLVDGQVAWLHVSGYPLLRADNPEAIVAFVDAVRRQGVPVSVDL
                     SSAGLIAAYGSGRFAELVRTMSPRLVFANESEWTALGLDASAVDFDLVIKRGAAGVTT
                     VIEGRATDHDAPPVDVVDLTGAGDALAAGHLVGGIDLGLATAARCVAQLGAQPTLAE"
     gene            complement(3041..3355)
                     /locus_tag="VH419_11820"
     CDS             complement(3041..3355)
                     /locus_tag="VH419_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEY7044347.1"
                     /translation="MWRHGLAYILQCSDGSFYVGSTTNLEARLWQHQRGEGAVYAARR
                     RPVTLVWSEHFDRIDEAFAFEKRVQRWGRAKRLALIEHRYADLPALAASRSQMTGDHC
                     GP"
     gene            complement(3593..4264)
                     /gene="purQ"
                     /locus_tag="VH419_11825"
     CDS             complement(3593..4264)
                     /gene="purQ"
                     /locus_tag="VH419_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007079481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEY7044348.1"
                     /translation="MKIGVVTFPGSLDDVDARRAARVAGAEPVALWHADHNLHGVDAV
                     VLPGGFSYGDYLRCGAIARFAPVMREVVDAAAAGMPVLGICNGFQILCESHLLPGALI
                     RNDHRKFVCRDQRLRIENTSTTWTNAYPPGAEVTIVLKNGEGGYVADAATLERLEGEG
                     QVVARYLEVNPNGSLRDIAGITNDRGNVVGLMPHPEHAVEPLCGAGTDGLTFFTSVLT
                     HLTSV"
     gene            complement(4261..4509)
                     /gene="purS"
                     /locus_tag="VH419_11830"
     CDS             complement(4261..4509)
                     /gene="purS"
                     /locus_tag="VH419_11830"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013160331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HEY7044349.1"
                     /translation="MARVVVDVMPKPEILDPQGKAVHDALPRLGFGGILDVRQGKRFE
                     LEFEGEITEERLAEVRRAAETLLSNPVIEDYTVRVEQS"
     gene            complement(4596..5231)
                     /locus_tag="VH419_11835"
     CDS             complement(4596..5231)
                     /locus_tag="VH419_11835"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY7044350.1"
                     /translation="MKQVKERFHVQILEGYGLSETSPVATFSHLDRPPKPGSIGTPIW
                     GTEVKLIDEDWKTIDGVDEIGEIAIRGHNIMKGYYKRPDATADVMRDGWFRSGDLARR
                     DEDGYYYIVDRAKDMIIRGGFNVYPREIEEVLVTHEAVSLAAVVGVPDDEHGEEVKAY
                     VIRADGADITEDELIDWSKEQMASYKYPRSIEFRDELPMTATGKILKRELS"
     gene            complement(5374..6144)
                     /locus_tag="VH419_11840"
                     /pseudo
     CDS             complement(5374..6144)
                     /locus_tag="VH419_11840"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
     gene            complement(6232..7134)
                     /locus_tag="VH419_11845"
     CDS             complement(6232..7134)
                     /locus_tag="VH419_11845"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017934403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEY7044351.1"
                     /translation="MTASTGDAHQVASITSLPGLKHLNSGKVRELYELPDGQLLMVAS
                     DRISAFDFVLTPTIPDKGEILTRMSLWWFEQLRALVPSHVVSTDVPSEVAGRAIVCER
                     LDMYPIECVARGYLAGSGLVDYQATGEVCGIELPAGLEDGSRLPDPIFTPATKGALGD
                     HDENVDYDAVVATVGDDIAAELQTLTLMIYERAEAIARERGIILADTKFEFGARPDGT
                     IVLGDEVLTPDSSRFWPVDEWQPGRAQTSYDKQFVRNWLLSAESGWDRSSGEPPPPLP
                     DTVVEQTRAKYVEAFERLTGDDFR"
     gene            7240..>8025
                     /locus_tag="VH419_11850"
     CDS             7240..>8025
                     /locus_tag="VH419_11850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY7044352.1"
                     /translation="MPDAVVVGSGPNGLVGANVLADHGWTVVVLEAQDEPGGAVRTDS
                     AVQPGFRHDTFSAFYPLAVASPAFRSLGLERYGLEWMRAPAVLGNPLPDSGWALLHRD
                     PERTAAGLDTCHRGDGAAWLRLCQKWEGFGPDLVGAALSPFPPVRSSTRAATGLLRHL
                     GPAGLTLPLQSVRRLAGSTFGGEAARLLLAGNALHADFSPHDLGSGTFGLILAMLGQT
                     VGYPVPRGGAAALTQALVRRLESRGGSVVTRSEVAGVVIRAGRC"
BASE COUNT         1244 a         2664 c         2788 g         1329 t
ORIGIN      
        1 ggacatgtgc gggaacaggt tgtacgcctg gaagacgatc ccgacgtcgc ggcgtacgtc
       61 gtcggggtcg acgcgcgggt cggtgatgtc gcggtcgtcc agccagatcc tgccgtcgtc
      121 gaccgtctcg agcaggttga cgcaccgcag cagcgtcgac ttgcccgagc cggatgcgcc
      181 gatcaggcag accgcctgct gctcctcgac gtcgaggtcg ataccgcgga ggacatggtg
      241 cggcccgaag tgcttgcgca ccgcgtccag gcggagcagc gtcatcaggc cgcgccctgc
      301 tcgcgccgca gctggcgcag cgccagccag tccgcgagcc gcgccagcgg gaccgtcacc
      361 acgacgaaga agagcgccgc gacgacgtac gcggtgaagt tgaagtcgcg gctggagtcg
      421 atctgcgcct gccgcaagat ctcgacgagt ccggccgtcg agacgagtgc ggtgtccttc
      481 tggagcgaca cgaagtcgtt gagcaacggc ggcatgaccc gtcggatcgc ttgcggcagc
      541 acgacgtggc gcaggctccg ctggtagctg agtccgagcg accgggccgc cgcccactgg
      601 ctcggatgaa ccgaggcgat tcccgcccgg accacctcgg cgacgtacgc accgtacgac
      661 agcgtcaatg caatgacccc tagccagaag atgctcgtcg gcgtgccctg caggtcgagc
      721 gccggtatgc cgaagcagac aaggaagatc acgagcaggg tcggcgtacc gcggaacaca
      781 tccgtgtaga tgatggcgag cagcttcagc ggtgccatcg ccggcgacgg caccacccgg
      841 atgatggcca cgacaagcgc gacgaccagg atgaagacct cggcgacgag gaacagcttg
      901 atgttgagca cgaaagcgcg agccagctcg ggaaacacgt cacgggcgta gccgacatcg
      961 aagtaggtct gctgcacgcg ctcccaaccc ggcgccgtga cgacgaggat gccgaggaga
     1021 gccagcacga ccaccgtcga cacggcggcg gtggccccac gccggagtgc ccggcgccgg
     1081 cggtactcga gtcgtccctc ctgaagcgtg ctgggacgcc actcctcagc atgaccggtg
     1141 gtgcgaacga cgtccgacat cggtcagctg agcgtcggga cgtcggcact ctggctcagc
     1201 caccgttgct ggaacttcgc cagtgtgccc ttgtccttca gcgtcgacag cgcctggtcg
     1261 gcgcactcga cgagcgggtt gcccttctcg aacagcagcc cgaactcttc cggctggccg
     1321 acggtcgact ggaactgtcc cacgatcgtg ctccccggga tctgggcggc ggtgatgtag
     1381 aaggccgttg gcaggtccgc cacaatcgcg tcgacctggc cgttctggag cgcgttcttc
     1441 gccaggttcg tgtcgtcgaa cacctgcggc tgagccgtcg tctggatcgt gtcggtgatc
     1501 gcgctcaacg acgtcgttcc cacctgggcg ccgagcgtcg cgtccttgaa atcggccagc
     1561 gacgttgcat cggcgtacgg cgaatccttc atcgtgatga tcgcctggtc ggcggtgtag
     1621 tacccgtcgg agaacgtcac ggccttcgcc cgctgggcgc tgatcgagat ctggttgatg
     1681 tcgaagtcga acttcttggg tcccggctgg tagctggaat tgaacggcac ccgcgtccat
     1741 tcgacctggt cgtcgctgaa gcccatcttc tcggctacgg cgtaggcgac tgcagactcg
     1801 aaacctttct tgttggtcgg gtcgttgtcg accatccacg gctcgaaggc ggggtcgtcg
     1861 gtcgcgatcg tgaggacgcc tggcttgaac gtctgtggat cggcgcacgc atcggtcgtc
     1921 gaggctccgg atgaggtctc tggcgaggtc gtggcgccgc cggagtcgtt gttgtcgtcg
     1981 ttgggggcgc agccgacaga gaggaggcag agaacagaga cgccgaccac gaatgccggc
     2041 gagagggcac gctgtcgcat ggcgccatcg tagggctgtc accctgggtg ccagcgggtg
     2101 tagtcgaggc gcggatgccg cccaccacgg gaggaagcga tgcacatcgt cacggtgggc
     2161 gacgtggtgc tcgacatcct cgtgaatgct ccgcgtggac tgcgtactga cgacgacacc
     2221 gaggccagga tcgccctcgc cgccggcggg caggcggcca acgtcgcgac ctggtcggcg
     2281 gcactgggtg cgcgggccac ggtgatcggt ccgaaggggc aggacattgc cgccggctac
     2341 atcgagtccg gtctcgcccg ccgcggggtc gacatgatcc cgatcgatgt cagtgccacg
     2401 gggaaggttg tcgccattgt caccggcagg gagcggacgc tcgcctcgga cgcgggagac
     2461 cagagttggg ccgcccgact cgatccgaac ctggtggacg ggcaggtggc ctggctgcac
     2521 gtgtcggggt acccgctgct gcgcgctgac aacccggagg ccatcgtcgc gttcgtcgac
     2581 gccgtacgcc gccagggggt gccggtctca gtcgaccttt cgtcggccgg actcatcgcg
     2641 gcatacggct ctggtcgctt cgccgagctg gtgcggacga tgtcgccgcg gctggtcttc
     2701 gccaacgagt ccgagtggac ggcgcttggg ctggacgcga gtgcggtcga cttcgacctt
     2761 gtgatcaagc gcggagcggc cggcgtgacc accgtgatcg agggccgcgc caccgatcac
     2821 gacgcgccac cggtcgacgt cgtggacctg accggcgccg gcgacgcgtt ggcggccggt
     2881 cacctcgtcg gcgggatcga cctcggcctc gccactgccg cccgctgcgt cgcccagctc
     2941 ggcgcccaac cgacgctggc tgaatgacag ccgacatccg ggggtctcga caagctcgac
     3001 cgccggatag gccgcagccc gctgatcgag cctgtcgaga tcaagggccg cagtgatcgc
     3061 ccgtcatctg gctgcgcgag gcggccagag ccggcagatc ggcgtaccgg tgctcgatca
     3121 gcgccaaccg cttcgcccga ccccatcgct gtacgcgctt ctcgaaggcg aaggcttcgt
     3181 caatccgatc gaagtgttcg ctccatacca gggtgacggg tcgacggcga gcggcgtata
     3241 cagcgccctc accacgctgg tgttgccaca gacgcgcctc caggttggtg gtactgccca
     3301 cgtagaacga tccgtcgctg cactgaagga tgtatgccaa gccatgccgc cacactgccg
     3361 aagcctggga acgggtcggc cggcttcgag agccttgtgg accgatgccg ggcagcgacc
     3421 agtcggggga tggacatcct cgaccttcac gcgctcgctg gttgtcgaac gaacgccgct
     3481 cgacacctgc ctccggaagg aggtctcgac aagctcgacc gccggacggc caggatctcg
     3541 accagctcga ccgccggacg gccaggatct cgaccagctc gatcgcgggg ggtcagacgg
     3601 aggtgaggtg ggtgaggacg ctggtgaaga acgtgagacc gtcggtgccc gcgccgcaca
     3661 gcggctcgac ggcatgctcg ggatggggca tgagaccgac gacattgccg cggtcgttcg
     3721 tgatgccggc gatgtcgcgc agcgaaccgt tcgggttgac ctcgagatat cgcgccacga
     3781 cctggccctc accctcgagg cgctcgagtg tggcggcgtc ggcgacgtac cctccctcgc
     3841 cgttcttcag cacgatcgtc acctcggccc ctggggggta ggcgttcgtc caggtggtcg
     3901 aggtgttctc gatgcggagc cgctggtcac ggcagacgaa cttccggtgg tcgttgcgga
     3961 tcagcgcgcc gggcagcagg tgggactcgc agaggatctg gaagccgttg cagatcccca
     4021 gcaccggcat gccggccgcc gccgcgtcca ccacttcgcg catcaccggc gcgaagcggg
     4081 cgatcgcgcc gcagcgcagg tagtcgccgt aggagaagcc gccggggagc acgaccgcgt
     4141 cgacaccgtg caggttgtgg tccgcgtgcc agagcgcgac cggttctgcg cccgccaccc
     4201 ttgccgcgcg tcgggcgtcc acgtcgtcca gcgaccctgg aaacgtgacg acgccgatct
     4261 tcacgactgc tccacccgga cggtgtagtc ctcgatgacg ggattgctga gcagcgtctc
     4321 ggcggcccgg cgtacctccg ccaaccgttc ctcggtgatc tccccctcga actccagctc
     4381 gaaccgcttg ccctggcgta cgtcgaggat gccgccgaag ccgagccggg gcagcgcgtc
     4441 gtgaacggcc tttccctgcg ggtcgaggat ctcgggtttg ggcatgacgt caacgacgac
     4501 gcgggccacg ggcactcctc ggcaagtcgg gcggcacacc ggacgcgcct cagtctagag
     4561 ggaccgcgcg cacctcccag ggccccgcgc ggacgtcagc tgagctcgcg cttgaggatc
     4621 ttgccggtgg cggtcatggg gagctcatcg cggaactcga tgctgcgcgg gtacttgtac
     4681 gacgccatct gttccttgct ccagtcgatc agctcgtcct cggtgatgtc ggcgccgtcg
     4741 gcccggatca cgtacgcctt gacctcttcg ccgtgctcgt cgtcgggtac gccgaccacg
     4801 gccgcgagcg agaccgcctc gtgggtgacg aggacctcct cgatctcgcg cgggtacacg
     4861 ttgaagccgc cgcggatgat catgtccttc gcccggtcga cgatgtagta gtagccgtcc
     4921 tcgtcccgcc gggccaggtc gccggacctg aaccagccgt ctcgcatcac gtcagcggtc
     4981 gcgtccggcc gcttgtagta gcccttcatg atgttgtggc cgcggatcgc gatctcgccg
     5041 atctcgtcga cgccatcgat cgtcttccag tcctcgtcga tgagcttcac ctcggtgccc
     5101 cagatcggcg taccgatcga gcctggcttc gggggacggt ccagatgact gaacgtggcc
     5161 acgggcgacg tctccgagag gccgtacccc tccaggatct ggacgtggaa gcgctccttg
     5221 acctgcttca tgatctccac cggcagcgcg gagcctccag cgacggcacg gcgcaaagtt
     5281 accggcgatc ttgtcgacgt cgacgctgtc cgtgagcgcg ttgagcaggc cccagtacat
     5341 cgtgggaacg ccggcgaaga acgtcaccga ctccttctcc atcaacgcca acgcctgctt
     5401 ggcatcgaag cgcgccatca ggaccaaggt ggaaccgagc ccgaagccgg cgttcagctg
     5461 cacggtctgc ccgaacgagt ggaacagcgg gagtgcgacg aggtgggtgt ccggccgcag
     5521 cgcactcagc tcccactccc ggtggcaggt gatcgcattg acggtgatgt tggagtgcga
     5581 cagctcagcg cccttcggct gcccggtcgt gccgctggtg tagaggatga cggcggtgtc
     5641 ggtcggctcg gtgaccaccg tctcgaacgt cgcggcctga ccggacgtgg ccgcgaagta
     5701 gctctccgcg ccgtcgatga catcggcggt cgggtcggcg gggatcacga agaagtgctc
     5761 gcaggtcggc gtggcgtcat acccctcctt gccggcctgt gccatcggca gctcgggggt
     5821 gccctcgaag cagaagtacg ccttcgcgtc tgagtcgtcg aggtggtagg cgacctcgcg
     5881 gcccttgaac aggacgttca acgggacgac cacggctccg gccttgagga tgccgtagta
     5941 gacgatgggg aagtacggca ggttcgggca cgagagggcg accttgtcgc cgggttcgat
     6001 cccgcgggag accaccaggt tggccacctg gttggccatc gcgtcgacct tcgcgtagct
     6061 gaggcgggtt tcgttgaaca ccacggcgtc gcggtcgggc accatgcggg cgctgtcttc
     6121 gagcatcacg gcgaggttga gcatgatgac cctccgttgc ggtcgcgcgg gttgcctaca
     6181 acgtagaccc ttcgacaagc ccgggacagg ccgttcgcga gggcgcagac gtcaccggaa
     6241 gtcgtcgccc gtcaggcgct cgaaggcttc gacgtacttc gccctggtct gctcgacgac
     6301 ggtgtccgga aggggtggtg gcggttcgcc ggaggagcgg tcccagccgg actcggccga
     6361 gagcagccaa ttgcggacga actgcttgtc gtacgacgtc tgggcccggc ccggctgcca
     6421 ctcgtcgacc ggccagaagc gtgacgagtc cggggtcagc acctcgtcac cgagcacgat
     6481 cgtgccgtct ggtcgtgccc cgaactcgaa cttggtgtcg gccaggatga tgccgcgctc
     6541 ccgggcgatc gcctcggcgc gctcgtagat catcagcgtc agggtctgca gctctgcagc
     6601 gatgtcgtca ccgacagtcg cgacaacagc gtcgtagtcg acgttctcgt cgtggtcgcc
     6661 gagcgctccc ttggtggctg gggtgaagat cgggtccggc agccgcgacc cgtcctcgag
     6721 ccctgccggg agctcgatcc cgcacacctc gccggtcgcc tggtagtcga cgagcccaga
     6781 cccggccagg taaccgcggg cgacgcactc gatcgggtac atgtcgaggc gttcgcacac
     6841 gatggcccgc cccgcgacct cgctgggcac gtccgtggag acgacgtgac tcgggaccag
     6901 tgcccggagc tgctcgaacc accacagcga catccgggtc aggatctcgc ccttgtcggg
     6961 gatcgtcgga gtgaggacga agtcgaacgc cgagatgcgg tcggacgcca ccatcagcaa
     7021 ctggccgtcc ggcagctcgt acagttctcg caccttgccg gagttgaggt gcttcaagcc
     7081 aggcagagac gtgatcgacg caacctggtg ggcgtcgccg gtactcgcgg tcatgccacc
     7141 atcctgccgt gctcggcggt cgagctcgtc gagacacgac ccgtgatctc gacgggctcg
     7201 accaccgggt tcgcgtgatg atggcggtca ggaggacacg tgccggatgc cgttgtggtc
     7261 ggttcagggc cgaatggcct cgtcggggca aacgtgctgg ccgaccacgg ctggacggtc
     7321 gtggtcctgg aggcgcagga cgagcccggc ggcgcggtcc gtacggactc agcggtccaa
     7381 ccagggtttc gccacgacac gttcagcgcg ttctacccgc tcgcggtggc atcgcccgcg
     7441 ttcaggtcgc tggggctcga gcgctacggc ctggagtgga tgcgcgcgcc cgccgtgctc
     7501 ggcaacccgc tcccggactc gggctgggca ctgctgcacc gcgacccaga gcgcaccgcc
     7561 gcggggctcg acacctgcca tcgcggtgac ggtgcagcct ggttgcggct gtgtcagaag
     7621 tgggagggct tcggcccgga cctggtggga gcggccctga gcccgttccc gccggtgcgc
     7681 tcctcgaccc gcgccgccac cggtctgctt cggcatctcg gcccggccgg tctgaccctg
     7741 cccttgcagt ccgtacgtcg cctggccggg tcgacgttcg gcggcgaggc ggcccggctg
     7801 ctgctggccg gcaacgcact gcacgcggac ttctcaccgc acgacctcgg ctccgggacc
     7861 ttcggtctca tcctggcgat gctcgggcag acggttgggt atccggtccc gcgtggcggg
     7921 gcggccgctt tgacgcaggc cctggtacgc cggctggagt ctcgcggcgg ctccgtcgtc
     7981 acacggtcgg aggtggccgg cgtggtcatc cgagccgggc gctgt
//
LOCUS       DATBFZ010000392         6880 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_603336, whole genome shotgun sequence.
ACCESSION   DATBFZ010000392 DATBFZ010000000
VERSION     DATBFZ010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 6880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6880
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_603336"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            210..2054
                     /locus_tag="VH419_11855"
     CDS             210..2054
                     /locus_tag="VH419_11855"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY7044353.1"
                     /translation="MKRPDVVDLHASSDAELIAAVRGGDSDAYGQLFERHRDAALRLA
                     RQIAGPSDADDLVSDAFVKVFRVLSAGGGPDIAFRAYLLTAVRRLHVDKIRRTSKVTP
                     TDELESYDAGVPFTDPAVQEFENVAASKAFASLPERWQLVLWHLEVEGQKPAEVALLL
                     GMSANSVSALAYRAREGLRQAYLQMHMADTAAEECRWVTERLGARVRNGLSRRDKVKV
                     DEHLDQCARCSAIYLELSEVNSNLAGVLAPILLGAAATGYLASTGGAAGATGLAAAAA
                     RTREFVKTHGGLTAASTAAGVVAVAVAAAVVMHAGGAAPRDTASDGPRAVVPVTSSST
                     SSDTTEASSGPTVSSSTGPGSDKPSVGGPTQNFAPPFNPPFTPPLVLPPTTPGSQPTL
                     TAPATSSPSETTSQSPPGSTSTAPPSTVPTTPTSSTTTAPTTEPPTTEPTSTEPTTSQ
                     PTTTTTTTSTTEPTTTPTTPTTPTTSTTTTTTTTTTTPPTATLAVGLTGHFIGDNPDG
                     RAFMLVADVQTTGDPSPITLRVRADEGAILPLPATVRAGDWHCDGFPLAGWRCTTSNP
                     TPGQITFIGTFFTADGQVSVTASAANAEDSSAVWPERESRLGTTRGND"
     gene            2277..3620
                     /gene="aspS"
                     /locus_tag="VH419_11860"
     CDS             2277..3620
                     /gene="aspS"
                     /locus_tag="VH419_11860"
                     /EC_number="6.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008358819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA(Asn) ligase"
                     /protein_id="HEY7044354.1"
                     /translation="MTAVSASSSASTSALEPPRTLAADLSTYADEPGRHIRMSGWIHR
                     RRRLSALSFVVLRDRSGTAQIVVKDADTLAQVETLSEETTVDVVGLVSPNAQAPGGVE
                     LVDPAFTALTDPAATPPVELWRPKLSAGLPTLLDHAQVTLRHPLRQAAWQLSAASLHG
                     FRTALDAGGFTEVQSPKLVESATESGANVFAVDYFGRPAYLAQSPQFYKQVMVGVFER
                     VYEIGPVFRAEPHDTVRHLAEYVSLDVELGFIADHREVIAVLRDVVAGMVGAIEERAP
                     RAVELLGLDLPAVPDEFPILHFRDALEIAGAPVDEPDLAPAHERALGEWAKAKHGSDF
                     VVVEGYPTAHRAFYSHPDPADPQWSRSFDLIFRGIELVSGAQRLHRYDDYVTTLAQRG
                     LPQKPYQGYIDAFRYGIPPHGGFAIGLERWTARLLEADNVREVTLFPRDLHRLSP"
     gene            3655..4152
                     /locus_tag="VH419_11865"
     CDS             3655..4152
                     /locus_tag="VH419_11865"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEY7044355.1"
                     /translation="MTLVRRIARPMLSSTFVVEGMNALKSPEARAQAAEPVIEKLHPA
                     VDKATDPLPFDLDNKQLVQLNGAVQVVGGLMLATGRWSRLSAFALAATLVPTTFAGHR
                     FWEETDPQRKNQQRIHFFKNVSMLGGLLLAAVDTEGKPSVAWRAKRGARRAKERVSDI
                     NPLTS"
     gene            4335..5609
                     /gene="aroA"
                     /locus_tag="VH419_11870"
     CDS             4335..5609
                     /gene="aroA"
                     /locus_tag="VH419_11870"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007077265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEY7044356.1"
                     /translation="MTTGAVVPWVAPYADHPVTGRVTLPGSKSITNRALLLAALATEP
                     STITRPLVARDTRLMMAALRAMGVGLEEHRDDIVVTPSPISGPADVDCGLAGTVMRFV
                     PPVAALADGPVSFDGDERARQRPMVEMLDALRVLGVQLDPGATGLPFTIEGAGAVKGG
                     VVTIDASASSQFVSGLLLAAARYDEGVDVRHEGKPIPSQPHITMTVSMLRERGVIVDD
                     SEPNRWQVAPGRVSAISVEVEPDLSNAAAFLAAAVVTGGRVTVADWPRRTAQAGDALR
                     SILPMFGAVSELEDDGLTVTGPAEVRGVDVDLHDVGELTPVIAAVAACATTPSHLRGI
                     AHLRGHETDRIAAIASELNALGGDVAETEDGLRISPRPLHGGVFRTYHDHRMAQAAAV
                     LGLLVRGLAVDDIATTSKTHEGFAAQWQAFVS"
     gene            5606..6607
                     /gene="rsgA"
                     /locus_tag="VH419_11875"
     CDS             5606..6607
                     /gene="rsgA"
                     /locus_tag="VH419_11875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012922794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEY7044357.1"
                     /translation="MTTYEDIDPSQFDRPRRRTRPRTKERPTYADAVDGFVVTVDRGR
                     FTVRVRPDGADERTVTAMKSRPLGRKGVVVGDRVRVVGDVSGAVDTLARIVEVTDRTT
                     VLRRTADDDDPVERILVANADQLVIVTAVANPEPRPRLIDRALVAAYDAGLDPLLCIT
                     KVDLADPEVVLSTYRPLGVPYVVTQRGTDLAQVQERLSDRVSVLLGHSGVGKSTLVNA
                     LVPGAARTTGDVNVVTGRGRHTSTSVVALELPFGGWVMDTPGIRSFGLAHVDPRHLIH
                     AFDDLAALTDDCPRGCTHGESEPECALDDAIADGRVAADRVTSFRRLLASRERTEGD"
     gene            complement(6642..>6880)
                     /locus_tag="VH419_11880"
     CDS             complement(6642..>6880)
                     /locus_tag="VH419_11880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044358.1"
                     /translation="AKRANLLFTLVIIFAVIAVVAFVLTQMRNVNNVLVTGVSVLLVI
                     GAVAVAFQTYRVGDIGSKAVWNPTGDVKYGSSSN"
BASE COUNT         1115 a         2464 c         2294 g         1007 t
ORIGIN      
        1 agtcgccgac ggccgggacg gggtccgttt ctgcatgatg tcgaagagca ccatcaggcg
       61 ttcctttcgc gcacaatgag gtgtgttgga aagactggtc cgaagaaggg acgcggcttt
      121 cacggttctg tgacgcgaaa cgaaccggca ggattacgct cgtagacggc aatgcgtcag
      181 gaaagggtga ctctccgtca cgttcgtcca tgaaacgccc tgatgtcgta gacctccacg
      241 cctcgagcga cgccgagctc atcgctgcgg tccgaggcgg agacagcgat gcctacggcc
      301 agctgttcga gcgccaccgc gacgccgcgc tgcggctcgc ccgccagatc gccggaccca
      361 gcgacgccga cgacctcgtc tcggacgcct tcgtcaaggt cttcagggtg ctctccgcgg
      421 gtggtgggcc ggacatcgcg ttccgcgcct acctgctgac cgccgtacgc cggctgcacg
      481 tcgacaagat ccgtcggacc tcgaaggtca ccccgaccga cgagctcgag tcgtacgacg
      541 ccggcgtccc cttcaccgat cccgccgtcc aggagttcga gaacgtcgcc gcctcgaagg
      601 cgttcgcctc gcttccggag cggtggcagc tcgtgctgtg gcacctggag gtcgagggac
      661 agaaacctgc cgaggtcgcc ctcctcctcg ggatgtccgc gaacagtgtg tcggcgcttg
      721 cgtaccgagc ccgtgagggc ctgcggcagg cctacctgca gatgcacatg gccgacaccg
      781 ccgccgagga gtgccgctgg gtcaccgaac gtctcggcgc gcgcgtccgc aacggcttgt
      841 cccggcgcga caaggtgaag gtcgacgagc acctcgacca gtgcgcgcgc tgttctgcga
      901 tctacctcga gctgtccgag gtcaactcca acctcgccgg agtgctggcg ccgatcctgt
      961 tgggcgctgc cgcgaccggc tacctcgcgt cgaccggcgg cgctgcgggc gcgaccggcc
     1021 tggccgcagc cgcggcacgg acccgcgagt tcgtgaagac ccatggcgga ctcaccgccg
     1081 cttcgacggc tgcgggcgtg gtcgccgtcg ctgtcgccgc ggccgtggtc atgcatgccg
     1141 gcggcgctgc tccccgcgac accgcgtccg acggcccacg cgccgtcgta ccggtcacgt
     1201 ccagctcgac ctcgtctgat acgaccgaag cctcgtccgg accgaccgtc tcgagctcga
     1261 caggtccggg cagtgacaag ccgtcggtcg gcggacccac gcagaacttc gcaccgccct
     1321 tcaatccgcc cttcactcca ccgcttgtcc tcccgccgac cacgcccggc agtcaaccga
     1381 cgttgacggc tcccgcgacg tcaagtccgt ccgaaacgac aagccagtcc ccgccgggca
     1441 gtacgtcgac tgcgccgccg tccaccgtgc ccacaacgcc gaccagtagc accaccacgg
     1501 cgccgacgac cgagccgccg accactgaac cgaccagcac cgagccgacg accagccaac
     1561 ccacgacgac gacgacgacg acatcgacga cggagccgac gaccactccg acaaccccca
     1621 caactccaac aacaagcacc acgaccacca ccacgaccac caccacgaca ccgcccacgg
     1681 ccacacttgc ggtcggcctg accggtcact tcatcggcga caacccggac ggtcgcgcgt
     1741 tcatgctggt tgccgacgtc cagacgaccg gtgacccgtc gccgatcaca ctgcgggtgc
     1801 gcgccgacga gggcgcgatc ctcccgcttc ctgccacggt ccgcgccggt gactggcact
     1861 gcgacggatt cccgctggcg ggctggaggt gcaccacctc gaatccgact cccggccaga
     1921 tcacgttcat cggcacgttc ttcaccgccg acgggcaggt cagcgtaacg gcgtcggccg
     1981 cgaacgccga ggacagctcg gccgtgtggc ccgagcgcga aagccgtttg ggaaccactc
     2041 gcggcaacga ctagaatcgc cgactcggcg acgacggaga cgagtagtcg cgggagttcg
     2101 cacgtacaga gagccgccgg aagctgagaa ggcggacgtc gatcccgcgc cgaagacact
     2161 ccggagccga tggagtacaa gaggccggtg aatcccgccg gcgaaggtgt ggtggcacag
     2221 cgaactcgcc catcgcccac acctccgggc tcgcacgcaa cggaggtgaa actcaggtga
     2281 ctgctgtatc cgcaagctcg tcggcatcca cgtcggcgct cgaaccaccc cgcacgctgg
     2341 ctgcggacct ctcgacgtac gccgacgagc cgggccgaca catccgcatg agtggctgga
     2401 tccaccgacg ccggcggttg tcggcactct cgttcgtcgt cctgcgcgac cggtcgggca
     2461 ccgcgcagat cgtcgtcaag gacgccgaca cgctggccca ggtcgagacg ctgagcgagg
     2521 agaccacggt cgacgtggtc gggctggtga gcccgaacgc acaggcacca ggcggtgtcg
     2581 agctggtcga cccagcgttc accgcactca ccgacccggc cgccacgcca ccggtcgagc
     2641 tctggcgacc caagctctcg gccgggctgc cgaccttgct cgaccacgca caggtgacgc
     2701 tgcggcaccc gctgcggcaa gccgcctggc agctgtcggc cgcatcactg cacgggttcc
     2761 gcacggcgct cgacgccggc gggttcaccg aggtgcagag cccgaagctg gtcgagtccg
     2821 cgacggagtc cggcgcgaac gtgttcgcag tcgactactt cggccgtccg gcgtacctcg
     2881 cccagtcccc gcagttctac aagcaggtga tggtcggcgt cttcgagcgg gtctacgaga
     2941 tcgggccggt gttcagggcg gagccgcacg acaccgtccg ccaccttgcg gagtacgtct
     3001 ccctcgatgt cgagctgggt ttcatcgccg accatcgcga ggtcatcgct gtcctccgcg
     3061 acgtcgtcgc cggcatggtc ggggcgatcg aggagcgcgc gccacgcgcc gtcgagctgc
     3121 tcgggctcga ccttcccgcc gtacccgacg agttcccgat cctgcacttc cgggacgccc
     3181 tggagatcgc cggcgcgccc gtcgacgaac cagacctggc gccggcgcac gaacgggcgc
     3241 tcggcgagtg ggccaaggcg aagcacggca gcgacttcgt ggtggtggag gggtacccca
     3301 cggctcaccg agccttctac agccatcccg acccggccga cccgcagtgg tcacgttcgt
     3361 tcgacctcat cttccgcggc atcgagctgg tgagcggtgc gcagcggctg caccggtacg
     3421 acgactacgt caccacgctg gcgcagcgcg gcctgccgca gaagccgtac cagggctaca
     3481 tcgacgcctt ccggtacggc atcccgccgc acggcgggtt cgcgatcgga ctcgaacgct
     3541 ggacggcgcg gctgctggag gcggacaacg tccgcgaggt cacgctgttc ccgcgcgatc
     3601 tgcaccggct ttcgccgtga ggtaggacgc ggccgaatcg ggtacaaagg cgctatgaca
     3661 ctcgtccgac ggatcgcccg ccccatgttg tcgtcgacgt tcgtcgtcga gggcatgaac
     3721 gccctgaaga gcccggaagc tcgcgcccag gccgccgagc cggtgatcga gaagttgcac
     3781 cccgcggtcg acaaagcgac cgacccccta ccgttcgacc tggacaacaa acagctggtc
     3841 cagctcaacg gtgcggtcca ggttgtcggc ggactgatgc tcgcgaccgg caggtggtcg
     3901 cggctgtcgg cgttcgcgct cgcggcgaca ctggtgccga ccacgttcgc cggccaccgc
     3961 ttctgggagg agaccgaccc gcagcggaag aaccagcaac ggatccactt cttcaagaac
     4021 gtctcgatgc tcggcggcct gctgctcgcc gccgtcgaca cggagggcaa gccgagtgtt
     4081 gcctggcgcg cgaagcgggg tgcccggcga gccaaggaac gcgtctccga catcaacccg
     4141 ctcacatcct gaacccaccc ggctccacct gccgcgagcc gcttcccatc tgcttggctt
     4201 ccgcgaaatg cgggaaacgt gcggcattcc tgggggcgtc gacctgacct ttcccgcatt
     4261 tcgcgggaac caccggcctg gggtgtcggg ataaggtggg aggtcgtgag gagttcgggg
     4321 aaagtgctgc gcgcgtgacg acaggcgctg tcgtcccttg ggtcgcgccg tacgccgacc
     4381 accccgtcac cggtcgggtc accctccctg gctcgaagtc gatcaccaac cgggccctgc
     4441 tcctcgccgc gctcgccaca gaaccgtcca cgatcacccg gccactcgtc gcgcgagaca
     4501 cccggctgat gatggcggcg ttgcgcgcca tgggcgtcgg cctggaggag caccgcgacg
     4561 acatcgtcgt gacaccgtcg ccgatcagcg gtcctgccga cgtcgactgc ggactggccg
     4621 ggaccgtcat gcgcttcgtg ccgccggtgg ctgcgctcgc cgacgggccg gtctccttcg
     4681 acggcgacga acgcgcgagg cagcgaccga tggtggagat gctcgacgcg ctgcgcgtcc
     4741 tcggcgtaca gctcgatccc ggagcaaccg gtctgccgtt cacgatcgag ggcgccggcg
     4801 cggtgaaggg aggcgtcgtg acgatcgacg cctcggcatc gagtcagttc gtctccggcc
     4861 tgttgctggc cgccgcacgc tacgacgagg gagtcgacgt gcgtcacgag gggaagccga
     4921 tcccgtcgca gccgcacatc accatgacgg tgtcgatgct gcgcgagcgc ggcgtgatcg
     4981 tggacgacag cgagccgaac cgctggcagg ttgccccagg ccgggtcagc gcgatctcgg
     5041 tcgaggtcga acccgacctc agcaacgccg cggcgttcct ggccgcggcc gtggtgacag
     5101 gtggccgggt gaccgtcgcg gactggccac gtcggacggc gcaggccggt gacgcgttgc
     5161 ggtcgatcct gcccatgttc ggcgcagtct ccgagctcga ggacgacggc ttgacagtga
     5221 ccggtccagc cgaggtgcgg ggggtcgacg tcgacctcca cgacgtcggg gagctcactc
     5281 cggtcatcgc cgcggtcgcc gcgtgcgcca cgacgccgtc gcaccttcgt ggcatcgccc
     5341 atctgcgcgg acacgagaca gaccggatcg ccgcgatcgc ctccgagctg aacgcgctgg
     5401 gcggtgacgt ggccgagacc gaggacggac tgcggatcag cccccggccg ttgcacggcg
     5461 gggtcttccg gacctaccac gaccatcgga tggcgcaggc ggctgcggtg ctcggcctgc
     5521 tggtgcgggg gctcgcggtc gacgacatcg cgaccacgtc gaagacgcac gagggcttcg
     5581 ccgcccagtg gcaggccttc gtttcgtgac cacgtacgag gacatcgacc ccagccagtt
     5641 cgacagacct cgacgtcgta cccggccacg aaccaaggag cggccgacgt acgccgacgc
     5701 ggtcgacggg ttcgtcgtca cggtggaccg cggccgcttc acggtacgag tccgcccgga
     5761 cggtgcggac gagcgcacgg tcactgcgat gaagtcgcgg ccgttgggcc gcaagggcgt
     5821 cgtggtcggg gaccgtgtcc gggtggtggg cgacgtgagc ggtgctgtcg acacgctggc
     5881 ccgcatcgtc gaggtgaccg accgcacgac ggtgctccgg cgtaccgcgg acgacgacga
     5941 cccggtcgag cgcatccttg tcgcgaacgc cgaccagctc gtcatcgtga cggcggtcgc
     6001 caacccggaa ccacgtcctc ggctcatcga ccgagcgctg gtggcggcgt acgacgccgg
     6061 gctcgacccg ctgctgtgca tcaccaaggt cgacctcgcc gacccggagg tggtgctgtc
     6121 gacgtaccgt ccgctcggtg tcccctacgt cgtcacccaa cgcggtaccg acctcgcgca
     6181 ggtccaggaa cggctcagtg atcgggtgag tgtgcttctc ggccattcgg gcgtcgggaa
     6241 gtcgaccctc gtcaacgcgc tcgtgccggg cgctgcccgc acgacgggtg acgtcaacgt
     6301 ggtcaccggg cggggcaggc acacctcgac atctgtcgtc gcgctcgaac tgccgttcgg
     6361 cggctgggtc atggacacac cagggatccg ctcgttcggt ctggcgcacg tcgacccgcg
     6421 ccacctgatc cacgcgttcg acgacctcgc cgcgctcacc gacgactgcc cgcgcgggtg
     6481 tacgcatggc gagtccgagc ccgagtgcgc cctggacgac gcgatcgccg acggccgcgt
     6541 ggccgccgac cgggtcacgt cgttccgacg actgctcgcc agccgagaac gcaccgaggg
     6601 cgactgaccg cggaatgcgg gaggaacctc ccgcggcggt gtcagttgct gctgctgccg
     6661 tacttgacgt cgccggtcgg gttccagacc gccttcgaac cgatgtcgcc gacgcggtag
     6721 gtctggaagg ccacggcgac cgcgccgatg acgagcagca ccgacacacc ggtcaccagg
     6781 acgttgttga cgttccgcat ctgggtcagg acgaacgcga ccacggcgat gaccgcgaag
     6841 atgatgacca acgtgaagag caggttggcc cgcttcgcat
//
LOCUS       DATBFZ010000393         1731 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_269408, whole genome shotgun sequence.
ACCESSION   DATBFZ010000393 DATBFZ010000000
VERSION     DATBFZ010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269408"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(258..1484)
                     /locus_tag="VH419_11885"
     CDS             complement(258..1484)
                     /locus_tag="VH419_11885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016456901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY7044359.1"
                     /translation="MSENPVSDHAAPASSAPQDKVRLGIIGLGAQGSMYASFIQQGMV
                     PNMVIGAIADVDPAKREKAAEYGVPFYEDYQTLLASGDVDAVVTCVPHYLHPEMGIQA
                     LQAGIHALVEKPAGVYTKQVKALNDFAASKPELTFGIMFNQRNNPLYQRIKEIVEAGE
                     IGAIRRTNWVITTWWRPQGYYEQSAWRATWGGEGGGVLVNQAPHQLDLWQWICGVPTS
                     VYAKVAYGFRRDIAVEDEVTALVDYGDGATGVFVTAVHDLVGTDRFEILGDKGKIVVD
                     GSKTATVTRLVKDELELSAAMDMEDVRKLFMGELDPSQYYTQEVLEFDSPWGQQHSGV
                     LENFAANILDGTPLLAPGSDGINGVRLANAIHLSSWLGKEIPLDFDEDDYLRELNHRI
                     RAEGKFSEREAQPTTA"
BASE COUNT          271 a          561 c          566 g          333 t
ORIGIN      
        1 gaggatctcg aaccggtcgg tcccgacgag gtcgtggacg gcggtcacga agacgccggt
       61 ggcgccggct tccgcctccg gtaacgcgcg tgtgcggcgc aagtctccac gcgaccgacg
      121 ctccgacaag atcaatggca ggtgaacttc tctctcgttc ttgccgacct ccggcagctc
      181 gcctccagat gttcgcgccg acgcgagagc tggtgtctca gccgttgccg ctgggtctga
      241 acagctgaaa ccgaacgtca cgcggtcgta ggctgtgctt cccgttccga gaactttccc
      301 tcggcacgga tccggtgatt gagctcgcgc aggtagtcgt cctcgtcgaa atccagcggg
      361 atctccttgc cgagccagga ggacaggtgg atggcgttgg ccaggcgcac gccgttgatc
      421 ccgtcgctgc cgggcgcgag cagcggcgtg ccgtcgagga tgttcgcggc gaagttctcc
      481 agcacgccgg aatgctgttg gccccacgga gagtcgaact cgagaacttc ctgcgtgtag
      541 tactggctcg ggtcgagctc gcccatgaag agcttccgga cgtcctccat gtccatggct
      601 gcggagagct cgagctcgtc cttgaccaga cgggtgacgg tggcggtctt ggagccatcg
      661 acgacgatct tgcccttgtc gccgaggatc tcgaaccggt cggtcccgac gaggtcgtgg
      721 acggcggtca cgaagacgcc ggtggcgccg tcgccgtagt cgaccagggc ggtgacctcg
      781 tcctcgaccg cgatgtcgcg tcggaagccg taggcgacct tggcgtacac agatgtgggc
      841 acaccgcaga tccactgcca caggtccagc tggtgcggag cctggttgac cagcacgccg
      901 ccgccctccc caccccaggt cgcacgccag gccgactgct cgtagtagcc ctgcgggcgc
      961 caccaggtcg tgatgaccca gttcgtgcga cggatcgcac cgatctcgcc ggcttcgacg
     1021 atctccttga tccgctggta gagcgggtta ttccgctggt tgaacatgat cccgaacgtc
     1081 agctccggtt tgctcgcggc gaagtcgttg agcgccttga cctgcttggt gtagacgccg
     1141 gccggcttct ccaccagcgc atggatcccc gcctggagcg cctgaatgcc catctcgggg
     1201 tgcaggtagt ggggcacgca ggtgacgacg gcgtcgacgt cgccgctggc cagcagggtc
     1261 tggtagtcct cgtagaacgg gacgccgtat tctgccgcct tttcgcgctt ggcgggatcg
     1321 acgtctgcga tcgcgccgat caccatgttc ggcaccatgc cctgttggat gaagctcgcg
     1381 tacatggatc cctgtgcgcc cagcccgatg attccgagcc tgaccttgtc ttggggcgct
     1441 gagctcgcgg gcgctgcatg gtcggacact gggttttcag acatcggcgg ttctccgttc
     1501 attccggttg ttcctctcaa cactgcaggt tatggcgccg acagccaacc ggttgccgtg
     1561 agttgccccg cgaggcccgg ctagccgggt cagccgcgga tcggtgtcga cggtgacaac
     1621 atgtggcaac acctgggcgg atgcccaccc gatcacgcac agttgaatgg tgattgcgac
     1681 cccgcccacg ctgaccaagg agcgacatcc gatgtggacc ctgtccggct t
//
LOCUS       DATBFZ010000394         5848 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_3225515, whole genome shotgun sequence.
ACCESSION   DATBFZ010000394 DATBFZ010000000
VERSION     DATBFZ010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 5848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5848
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3225515"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..802
                     /locus_tag="VH419_11890"
     CDS             <1..802
                     /locus_tag="VH419_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019047772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEY7044360.1"
                     /translation="IPEIQYLAYLHNAEDQIRGEGASLAFFSKGQYRNGMVIRVPGLA
                     YQKGFGGHFHNDNSLAVLRDIPGVVVAVPSHPAEVPALLRTCVGLAAAEGRVCVFVEP
                     IALYHERNLFAEDQAWLAPYRAASPSDPDRFAKVTTHGDGEDLLFVTFGNGVRMSRRA
                     AARLRDMGVSSTVLDLCWLAPLPYADLRDHVRRFRHVLVVDETRWSGGVAEGVLAALV
                     DARYGGTVQRLTSKNSFVPLGPAADLVLVSEDDIVATAMMMVRPASMG"
     gene            824..2329
                     /locus_tag="VH419_11895"
     CDS             824..2329
                     /locus_tag="VH419_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007078255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY7044361.1"
                     /translation="MTDLAGRTRDILDRLGVPDPFDDRGTLVARTPIDGSELGHLHEH
                     SADDVAEAVRAAQQAFEEWRDVPAPVRGQLVRELGGLLRQHKEDLGTLVSIEAGKIMS
                     EGLGEVQEMIDICDLAVGLSRQLFGRTIASERPGHRMMEQWHPLGVVGVISAFNFPVA
                     VWSWNAAIALVCGDAVVWKPSEKTPLTALACQSLTGEAVRRVGAPANVSSVLLGAASV
                     GEALVDDPRVALVSATGSTRMGKAIAPRVGGRLGRLLLELGGNNAAIVTPSADLDLAT
                     RAIVFSAVGTAGQRCTSLRRVIVHESVRDDLVERLRAAYTSLPIGSPLEPSTLVGPLI
                     DATALEAFGAAIRQASADGGDVVVGGEHAPVDGDGCYVRPAIVTMPSQTAIVRSETFA
                     PLLYVLSYDDFDDAIAMHNDVTQGLSSSIFTTDVREAERFVSATGSDCGIANVNIGPS
                     GAEIGGAFGGEKETGGGRESGSDAWKAYMRRTTNTVNSTSELPLAQGVEFL"
     gene            complement(2544..4184)
                     /locus_tag="VH419_11900"
     CDS             complement(2544..4184)
                     /locus_tag="VH419_11900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017935050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY7044362.1"
                     /translation="MTQPTKAASGIVLLTLASGQFLMTLDSSVMNVSIAQVAEDVGTD
                     VTGVQTAITLYTLVMATTMITGGKIGTMIGRRRAFAIGCVVYGMGSFTTALAPNLTVL
                     ILGWSVLEGLGAALIMPAIVALVASNFGPDERPRAYGLVASAGAIAVAVGPLIGGAVT
                     SFWTWRLVFAGEVVIVAGILVLTKKIHDTAAPQVAPRIDVVGVVLSATGLGLAVYGVL
                     RSGEWGWVLATDGQPSWLGLSPSLWLIVGGLLVVRAFFAWEDRVASSGREPLVDVTLF
                     RNARLVGGLTMFFFQFLIQAGLFFTVPLFLSVALGLSAIDTGIHLLPLSLTLLAAAVG
                     IPKFVPAASPRRVVSVGLAALLAGMISLIAALEAGAGGEIVTVPLLLAGLGVGALASQ
                     LGAVTVSAVPDSQSGEVGGIQNTMTNLGASLGTALAGSVLIAGLTSAFIDGVQDNPAV
                     PDSVAAAADTQLASGIPFVSDADLETALDEAGVPHDQADVILDTNVDARIAALRLSIA
                     VLAMVAIGALFCTRLLPKHAVGHDKVSVASPTEPAGNA"
     gene            complement(4224..5102)
                     /locus_tag="VH419_11905"
     CDS             complement(4224..5102)
                     /locus_tag="VH419_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020575926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY7044363.1"
                     /translation="MRLSISVTNYSWAADTPVRDGLATIARRADDAGLDTVWVPDHLV
                     QVDPTVPDDATDMLEAYTTLGYLAGLTERVRLGALVSNVALRTPALLVKAVSTLGVLS
                     GRRAWLGIGAGYRGDEAADMGFELPDTPERFERLEETLQIATKMFAGDDSAYEGTRYR
                     LARPRNMPRPQQPPAILLGGTGERRTLRLVARYADACNVFDIPDEGRTIRQKLAVLAE
                     HCESDGRPFDAIEKTLSTRIAPGESADELVRRAEWFATIGIEHLIFVSSDPWSADSME
                     LLAAAAPRVAGFVPAG"
     gene            complement(5129..>5848)
                     /locus_tag="VH419_11910"
     CDS             complement(5129..>5848)
                     /locus_tag="VH419_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008359525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ionic transporter y4hA"
                     /protein_id="HEY7044364.1"
                     /translation="VTFNAEGSGAALATITTLAVLTMVVPTFAISEPGPEFSPSQLVF
                     ASVMSLLLYAIFVVNQTGHHRDFFLPLATHGHAVEPHEHAAPPTNRVTWASFGLLLVS
                     LVAVVGLAKVESPAIEDAVEAAGFPTSFVGVVIALLVLLPETIAAVRSASRNRMQLSL
                     NLALGSAMASIGLTVPAIAVASIWLEGPLVLGLGGLQLVLLALTVVVSVLTVVPGRAT
                     RLQAGVHLVLFSAFVFLAINP"
BASE COUNT          982 a         1951 c         2005 g          910 t
ORIGIN      
        1 gataccggag atccagtacc tggcctacct ccacaacgcg gaggaccaga tccgtggtga
       61 gggtgcgtcg ctggcgttct tctcgaaggg gcagtaccgc aacggcatgg tgatccgggt
      121 ccctggcctc gcataccaga aggggttcgg tgggcacttc cacaacgaca actccctcgc
      181 cgtgctccga gacatccctg gtgtcgttgt cgcagtcccg tcacatcccg cggaggtgcc
      241 cgcgttgttg cgcacatgcg tgggactggc agccgcggaa ggtcgcgtct gtgtgttcgt
      301 ggagccgatc gcgttgtacc acgagcgcaa cctgttcgcg gaggaccagg cgtggctggc
      361 gccgtatcgt gctgcatcgc caagcgatcc cgaccggttc gcgaaggtca ccacacacgg
      421 cgacggcgag gacctgctct tcgtgacctt cggcaacggc gtacgcatga gccggcgtgc
      481 agcggcgcgg ttgcgtgaca tgggagtgtc gagcaccgtg ctcgacctct gctggctggc
      541 cccactgccg tacgccgacc tgcgtgacca cgtacgacgc ttccggcatg tcctcgtcgt
      601 cgacgagaca cggtggagcg gtggggtggc cgaaggagtc ctggccgcgc tggtcgacgc
      661 tcggtacggc ggcaccgtcc agcggctcac cagcaagaac agcttcgtcc cgctcggtcc
      721 ggcagccgac ctcgtgctgg tgtccgagga cgacatcgtg gccacggcga tgatgatggt
      781 gcggcctgcg agcatgggct gagacagagc gagggagcag acgatgaccg atctggccgg
      841 cagaacccgc gacatcctgg accgcctcgg cgtacccgat ccgttcgacg accgcggcac
      901 gctcgtcgcc cgtacgccga tcgacggcag tgagctcggc cacctccacg aacactccgc
      961 cgacgacgtg gccgaggccg tgcgagccgc gcagcaggcc ttcgaggaat ggcgagacgt
     1021 tccggcaccg gtgcgtggcc agctggtgcg tgagctcggc gggctgctgc ggcagcacaa
     1081 ggaggacctc gggacgctcg tgtcgatcga ggccgggaag atcatgtcgg agggcctggg
     1141 cgaggtccag gagatgatcg acatctgcga cctggccgtc gggctgtcgc gtcagctgtt
     1201 cggccgcacc atcgcaagcg agcgtcccgg ccaccgcatg atggagcagt ggcacccgct
     1261 cggcgtggtg ggagtgatct cggcgttcaa cttcccggtc gcggtgtggt cgtggaacgc
     1321 cgcaatcgca ctggtctgcg gtgatgcggt ggtgtggaag ccgtctgaga agactccgct
     1381 cacggcgttg gcatgtcagt cgttgacggg cgaagcggtt cggcgcgtcg gggcgccggc
     1441 caacgtgtcg tccgtgctgc tcggcgccgc gtcggtgggg gaggcgctcg tcgatgaccc
     1501 gcgcgttgct ctcgtgtcgg cgaccggttc gaccaggatg ggtaaggcga tcgcgcctcg
     1561 cgtcggcggt cggctcggcc ggctgctgct cgagctcggc ggcaacaatg cggccatcgt
     1621 gactccgtcg gccgatctcg acctcgcaac gcgtgccatc gtcttctcgg ccgtcggtac
     1681 ggcgggccag cgctgcacgt cactgcgtcg cgtcatcgtc cacgagtccg tacgagatga
     1741 cctcgtcgag aggcttcggg cggcgtacac gtcactgccc atcgggtcac cgctcgagcc
     1801 atcgacactg gttggtcccc tgatcgacgc gactgctctg gaggcgttcg gtgccgcgat
     1861 ccgacaggcg tcagccgacg gtggcgacgt ggtcgtcggc ggcgagcatg caccggtcga
     1921 cggtgacggc tgctacgtcc ggccggcgat cgtgacgatg ccttcacaga ccgcgatcgt
     1981 tcggtccgag acgtttgcgc cgctgctgta cgtgttgtcc tacgacgact tcgacgatgc
     2041 catcgcgatg cacaacgacg tgacgcaagg actgtcgtcc tcgatcttca ccacggacgt
     2101 ccgggaggcg gagcgattcg tctcggccac cgggtccgac tgcggcatcg ccaacgtgaa
     2161 catcggcccg tccggcgccg agatcggtgg tgcgttcggc ggcgagaagg agaccggagg
     2221 tggccgcgag tccggctccg acgcctggaa ggcctacatg cggcgtacga cgaacacggt
     2281 caactccaca tccgagctcc cgcttgcgca gggcgtcgag ttcctctgac tctgcgggcg
     2341 accgcgcgcg ggctcttcgt gctcgggccc gacgctagac gagtgattcc aagggaagtt
     2401 gcctggctcg cggcttcccg catcccgcct ggctgcgttg tcgtcactcc gaataggagc
     2461 tccgctatga gtcgctcctc cgccttgcca gatcgggcgc ggatgagccg ctcgctgcac
     2521 cggcacccct tggaatcaat cgtctaggcg ttgccggcag gttctgtggg cgacgcgacg
     2581 ctcaccttgt cgtgaccgac ggcgtgcttc ggcagcagcc gcgtacagaa caacgcgccg
     2641 atggcgacca tggccagcac tgcaatgctg aggcggagtg cggcgatcct ggcgtcgacg
     2701 ttcgtgtcga gaatgacatc ggcttggtcg tggggaactc ccgcctcgtc gagggcggtc
     2761 tccaggtcgg cgtcggaaac gaaggggata ccgctggcga gctgggtgtc agcggcagcg
     2821 gcaaccgagt cgggcaccgc agggttgtcc tgcacaccgt cgatgaacgc tgacgtcagg
     2881 cccgcgatga ggaccgaccc tgccagagcg gtccccagcg atgccccgag gttcgtcatc
     2941 gtgttctgga taccgccgac ctcgccgctc tggctgtctg gaacggcgga gacggtgacg
     3001 gctcccagct gcgaggcgag ggcaccgacc ccgagcccgg caagcagcag cgggaccgtg
     3061 acgatttcac ccccggcgcc cgcttcgagc gcggcgatca gcgagatcat cccggcaagc
     3121 agcgctgcga gaccgaccga caccacccgc cgcggcgacg cggccggtac gaacttcgga
     3181 atgcccaccg cggcggcgag cagcgtgagc gacagtggca gcagatggat cccggtgtcg
     3241 attgccgaca gtcccagcgc caccgagagg aagagtggca ctgtgaagaa gagcccggcc
     3301 tgaatcaaga actggaagaa gaacatcgtc aggccaccga ccagccgcgc gttgcggaac
     3361 aaggtgacgt cgacaagagg ttctctccca ctgctcgcga cccggtcctc ccaggcgaag
     3421 aacgcacgga cgaccaggag accgccgacg atcagccaga gcgagggtga cagcccgagc
     3481 cacgagggct gtccgtcggt ggccaggacc cagccccact caccggagcg cagtacgccg
     3541 tagaccgcca ggccgaggcc ggtggccgac aagacgacgc cgaccacgtc gattcgtgga
     3601 gcaacctgcg gggcagcggt gtcgtggatc ttcttcgtga gcacaaggat ccccgccacg
     3661 atcaccacct caccggcgaa gacgagccgc cacgtccaga agctcgtcac tgcaccaccg
     3721 atcaacggcc cgacagctac cgcgatcgca cctgcggagg ccaccaggcc gtacgcccgt
     3781 gggcgttcgt cggggccgaa gttcgacgcc acaagggcga cgatcgccgg catgatcagc
     3841 gcggcgccga gcccttcgag aacggaccag ccaaggatca acacggtcag attcggggcg
     3901 agcgctgtcg tgaaggagcc cattccgtag acgacgcagc cgatggcgaa cgcacgtcgg
     3961 cggccgatca tcgtgccgat cttcccgccg gtgatcatgg tcgtggccat cacgagggtg
     4021 tacagggtga tcgctgtctg caccccggtg acgtcggtgc cgacgtcctc cgcgacctgc
     4081 gcgatcgaga cgttcatgac cgaactgtcc agcgtcatga ggaactgtcc cgaggccaag
     4141 gtgagcagga cgatcccgga tgctgccttg gtcggctgag tcattcccac cacccctcct
     4201 gacgccgacg tacgccgatg atctcagccc gcagggacga agccggcgac gcgtggagca
     4261 gcggcggcca gcagctccat cgagtccgct gaccacgggt cgctggacac gaagatcagg
     4321 tgctcgatcc cgatcgtcgc gaaccactcc gcccggcgta ccagctcgtc cgccgactca
     4381 ccgggagcga tacgcgtgct cagcgtcttc tcgatcgcat cgaacggccg cccatcggac
     4441 tcgcagtgct ccgccagcac cgcgagcttt tgcctgatcg tccggccctc gtcggggatg
     4501 tcgaacacgt tgcacgcgtc tgcgtagcgc gcgacgagcc gcagcgtccg gcgttcgccg
     4561 gttcccccga gcaggatcgc cggcggctgt tgtgggcgag gcatgttgcg cggccgggcc
     4621 agccggtagc gggtgccttc gtacgccgaa tcgtcgccgg cgaacatctt ggtggcgatc
     4681 tgcaacgtct cctcgagccg ctcgaaacgc tccggggtgt ccggcagctc gaaccccatg
     4741 tctgcagctt cgtcgccgcg gtagccggca ccgatgccga gccaggcgcg tcggccggac
     4801 agcacgccca gtgtcgacac agccttgacc agcagcgccg gtgttcgcag ggctacattg
     4861 ctgacgagtg ctccgagtcg gacgcgctcg gtcagcccgg cgaggtagcc cagtgtcgtg
     4921 tacgcctcca gcatgtcggt cgcgtcgtcg ggcaccgtcg ggtcgacctg caccaggtgg
     4981 tccggcaccc acaccgtgtc cagcccggca tcgtccgcgc gacgcgcgat ggtcgccagc
     5041 ccgtctcgca cgggtgtgtc cgccgcccac gagtagttcg tcacgctgat gctcagtcgc
     5101 atccagccac ctcctaggtg cggcctggtc acgggttgat cgcgagaaac acgaacgccg
     5161 agaacaacac taggtgcacg ccggcctgga gccgggttgc ccggcccggt acgacggtga
     5221 gaacgctcac gaccacggtg agcgcgagca ggacgagctg cagcccgccg agacccagca
     5281 ccagcgggcc ttcgagccag atggaggcca cggcgatcgc cggcacggtc aggccgatgc
     5341 tggccatggc ggagccgagt gcaaggttga ggctgagctg catccggttg cggctcgcgg
     5401 agcgcacagc ggcgatcgtc tccggtagga gcaccagcaa ggcgatcacc acaccgacga
     5461 acgacgtcgg gaacccggcc gcttcgaccg cgtcctcgat cgcgggtgac tccaccttgg
     5521 cgagccccac cacggcgacg agcgagacga ggagcagccc gaaactcgcc cacgtcaccc
     5581 ggttcgtggg cggagccgca tgctcgtgcg gctcgaccgc gtgtccgtgg gtcgcaagtg
     5641 ggaggaagaa gtctcgatgg tggccggtct ggttgaccac gaagatcgcg tagagcagca
     5701 gcgacatcac tgacgcgaag acgagctgcg acggcgagaa ctcgggaccg ggttcgctga
     5761 tggcgaatgt cggtaccacc atcgtgagga ccgcgagcgt ggtgatcgtc gcgagggcgg
     5821 cgcccgaacc ctccgcgttg aacgtgac
//
LOCUS       DATBFZ010000395         5247 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_931422, whole genome shotgun sequence.
ACCESSION   DATBFZ010000395 DATBFZ010000000
VERSION     DATBFZ010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 5247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5247
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_931422"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..458
                     /locus_tag="VH419_11915"
     CDS             <1..458
                     /locus_tag="VH419_11915"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEY7044365.1"
                     /translation="LRWTEWVCHPNIANTRRAIRRCPRRQADLSGFTELQGNVGMLLA
                     LLVLSWTLAAFGEELAYRGYLLTRIRDVVGSGRVGTVSAIAVSSLLFGFAHTEQGIVG
                     AAIVTLDAVAFSVLRYRYKTLWASVLAHGFNNTLGFVAFFLVGPVHGLW"
     gene            623..787
                     /locus_tag="VH419_11920"
     CDS             623..787
                     /locus_tag="VH419_11920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044366.1"
                     /translation="MGPLRDPGAEPLLTAESVEKYYRNGEVSAHALVDLVPARRAAGI
                     RPALPVRVAD"
     gene            complement(836..1582)
                     /locus_tag="VH419_11925"
     CDS             complement(836..1582)
                     /locus_tag="VH419_11925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044367.1"
                     /translation="MAAFSVLFVTTGIAAASPDAPPSPNAPGGPSLRADAAAIAAGEA
                     GRLVRIVTPCRLLDTHKKGGPIRAGTVRKVTVVGKCGIPGSATAIIASVTTTASTSNG
                     YLTGYPTGTTRPKARFATYLKGQAMTVGATLPLRIGQEPSLSIFSSGRTHVIIDVTGY
                     YEEQIHAVLAPSGGIFNGTPRVVSSTHLGTGSYQVAIDRDTTGCTPIASVHGNAYFAS
                     SYESGGFIYANTYAPNGTPTDLYWSLTVLC"
     gene            1829..3082
                     /locus_tag="VH419_11930"
     CDS             1829..3082
                     /locus_tag="VH419_11930"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEY7044368.1"
                     /translation="MIAAALALGLAACGGGSDSDSGGKVTLDYWVYESTDPTSFSNAL
                     VDAFEKKYPNVEVKQTQFPYANYDVKLQTAIAAGNAPDLAYTFDLDLMRREQLLPLDD
                     MVKKYNIDISNFNQAIIKGPGTFSCTWEDKLYCLGSTQGGWSVFYNKDMFDAAGIPYP
                     AAWPPMTLEQFADIACRLTDKSKGVWGAAVPTDFLPRELSVSPDGKTVEGYINSPATI
                     HDYEVLAGIYQDGCSPTSNVIDPWDQSAQFFERGAIAMAMADFDIAKAVEHAGINYGV
                     TGPPTPPGVEPYFDVYSNNTGILASTDHPKEAEEFLAFMATKGQRIAWETEGSTPIDN
                     NVAKQVNWAQGIPGREDALEVLSHARSGIFLPDTGNTWGPFYDAWGYILSGDKSVEEA
                     INDATPALQQNLDKAWRDWDESAPD"
     gene            complement(3159..4271)
                     /locus_tag="VH419_11935"
     CDS             complement(3159..4271)
                     /locus_tag="VH419_11935"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY7044369.1"
                     /translation="MTDRPRFLYVSPREDGWWSTQSSRRYFHGGERYRGLADGVLSPH
                     GAIECLEWGVDDPAEARAEMLDAGLAHADALVASPWLWLGEGGTFDEARLARASRLKV
                     IAGTFDYRLSWIDLEAAARRGVTVVDTSRTMTWTVAEFALAITFALLRDIPRSIDLVR
                     SGGWQDSGKTGSTYLFRDLADSQVGLAGFGSINQHYRQFVEPYGCPVSVFDPLVDDEV
                     AAAHSVTRAVSLVELARSSDIFVVAIPPTPSTLGVIDAEVIEALAPGSLFVLVSRMAV
                     VEQEALWRRARAEELRVAVDVFDPEPPPRDAWFRGAANVLPTPHIAGNTPFAHERCLT
                     EACADAVRIVNGEAPYHAATTLDKRMYEGKVAVETR"
     gene            complement(4268..5113)
                     /locus_tag="VH419_11940"
     CDS             complement(4268..5113)
                     /locus_tag="VH419_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020579805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate hydrolase family protein"
                     /protein_id="HEY7044370.1"
                     /translation="MRAPLLDRFRAQLDGGGVLVGAGAGTGLSAKCAEAGGADLIIIY
                     NSGRYRMAGRGSLAGLMPYGDANAIVVEMAAEVLPVVRQTPVLAGVCGTDPFRVMSVF
                     LQQLKDIGFAGVQNFPTVGLVDGTFRQNLEETGMGYALEVEMIRQAHALDLLTTPYVF
                     DADDARAMTGAGADVLVAHMGLTTGGSIGAETGKTLDHCVGLIQEIRDSAVDVSPDVI
                     VLCHGGPISEPDDAAYVLARTSGVAGFFGASSMERLPTEVAITDTVRRFKELRPRDAQ
                     KETGT"
BASE COUNT          928 a         1746 c         1727 g          846 t
ORIGIN      
        1 acctccgatg gacggagtgg gtttgccatc cgaacatcgc aaacaccagg cgggccatcc
       61 gcagatgtcc gaggaggcag gcggacctca gcggcttcac tgaactccag gggaacgtcg
      121 ggatgctact cgcgctgctc gtcctcagtt ggacgctcgc ggcgttcggg gaagagctcg
      181 cctatcgcgg ctacctgctg acccggatac gcgacgtcgt cggctccgga cgagtaggga
      241 cggtgagcgc gatcgctgtg tcatcgctgc tcttcggctt cgcacacacc gagcaaggca
      301 tcgtcggtgc cgccatcgtg acgctggatg cggttgcctt cagcgtcctt cgctaccggt
      361 acaagacgct gtgggcgtcg gtgctcgcac atgggttcaa caacaccctc ggattcgtcg
      421 cgttcttcct cgtcggaccc gttcacggat tgtggtgacg ccgtttcgcc gaccgcagtt
      481 cggtgaccgc agcgcgcttc gcgcaatccg cgccgatggc agaggcgggt gacaggggac
      541 ttggtcctga tctgcgagga catccgctcc tgctccagct gcgccgcgag gccgatgctg
      601 gaaccgagga agggagcgtg ccatgggacc gctacgggat cctggtgcag agcctctgct
      661 caccgccgag tcggtggaga agtactaccg caacggtgag gtgtccgcgc atgcccttgt
      721 cgacctcgtt ccggcgcgcc gtgccgccgg catccggccc gccctgcctg tccgcgttgc
      781 cgactgacca ggcgcagttg cggcacagcg gccgcggatc gagggcgaac gcggctcagc
      841 agagcacggt caggctccag tacaggtcgg tcggtgttcc gttcggcgcg taggtgttgg
      901 cgtagatgaa tccgccggat tcgtaggaac tggcgaagta cgcgttgccg tggacggagg
      961 cgatcggagt gcagccggtc gtgtcgcggt cgatcgccac ctggtaagag cccgtgccga
     1021 ggtgcgtact cgacacgacc cgaggcgtgc cgttgaagat gccgccgctg ggggcaagca
     1081 ccgcgtggat ctgttcctcg tagtacccgg tcacgtcgat gatgacgtgc gtgcgacccg
     1141 aactgaagat cgacaggctc ggctcctgcc cgatgcgtaa cggcaaggtg gcgcccacgg
     1201 tcatcgcctg acctttcagg taggtcgcga accgggcctt cggccgggtg gttccggtcg
     1261 ggtacccggt gaggtagccg ttcgaggtcg aagccgtcgt cgtcacgctg gcgatgatgg
     1321 cggtggcgga tcccggtatc ccgcacttcc cgacaaccgt caccttccgg acggttcccg
     1381 ccctgatggg accgcctttc ttgtgggtgt cgagcaaccg gcagggagtc acgatgcgga
     1441 ccagccggcc tgcctcgccc gcagcgatgg cggcggcgtc ggcccgcagc gacggtccgc
     1501 ctggcgcgtt cggactcggt ggggcatcag gagaagcagc agcaatcccc gtggtcacga
     1561 agaggacgga gaacgcagcc accacggcaa gcttcgtact gcctcgacca gtccaggacc
     1621 gcgaggcggg agttgtcgtc atagcgcatt gtgcgcccgc tgccctcagt gagccagacc
     1681 tcatcagcgt cagagcgcac gacgacctgg tatcgttccc atattcggcc ggtaggggcg
     1741 cgctctcggc ggcacgtatg ccgccccagc ctcgagggag agagccatgg cggagttcgg
     1801 agtaaggacc cacgtacgac gaatctgtgt gatcgctgct gcactggccc tcgggctggc
     1861 cgcatgcgga ggcggctcgg attccgattc cggcggcaag gtgacactcg actactgggt
     1921 ctatgagtcg acggacccga cgtcgttcag caacgcattg gtggacgcgt tcgagaaaaa
     1981 gtacccgaac gtcgaggtca agcagaccca gttcccgtat gcgaactacg acgtcaaact
     2041 ccagaccgca atcgcggccg gcaatgcgcc agacctcgcc tacacattcg acctcgacct
     2101 gatgcggaga gaacagctcc tgccgctgga cgacatggtc aagaagtaca acatcgacat
     2161 ctcgaacttc aaccaggcga tcatcaaggg accaggcacg ttcagctgta cgtgggagga
     2221 caagctctac tgcctgggtt cgacgcaggg cggctggtcg gtcttctaca acaaggacat
     2281 gttcgacgcg gcggggatcc cataccccgc agcctggcca ccgatgacgc tcgagcagtt
     2341 cgccgacatc gcctgccggc tgaccgacaa gtcgaagggc gtgtggggtg ccgcggtgcc
     2401 gaccgacttc ctgccccgag agctgagtgt gagcccggat ggcaagacgg tcgagggcta
     2461 catcaacagt cctgcgacga tccacgacta cgaggttctc gccggcatct accaggacgg
     2521 ctgctcgccc acgtcgaacg tgattgaccc ctgggaccag tccgcgcagt tcttcgagcg
     2581 cggcgccatc gcgatggcga tggccgactt cgacatcgcg aaggcggtcg agcacgccgg
     2641 gatcaactac ggcgtgaccg ggccgccgac gccaccgggc gtcgagccct acttcgacgt
     2701 ctactccaac aacaccggga tcctggccag taccgaccac ccgaaggagg ccgaggagtt
     2761 cttggcgttc atggccacca agggccagcg catcgcctgg gagaccgaag gttctacccc
     2821 gatcgacaac aacgtggcga agcaggtgaa ctgggcacag ggtattccgg gccgagagga
     2881 cgcactcgaa gtgctgtcgc acgcgaggtc cgggatcttc ctgcccgaca ccggaaacac
     2941 gtggggcccc ttctacgacg cgtgggggta catcctctcc ggcgacaaga gcgtcgagga
     3001 agccatcaac gatgccacgc cggcactgca gcagaacctc gacaaggcct ggagagactg
     3061 ggacgagtcg gcgcccgact gatggacgga gccgagcgac gaagtcgccg gcttcggctc
     3121 aacaagtcac cgtcccctct tgatctcgac agactccatc agcgggtctc gacagccacc
     3181 ttgccttcgt acatgcgctt gtcgagcgtt gtcgcggcat gatatggagc ctcaccgttg
     3241 acgatgcgca cagcgtccgc gcatgcctcg gtgagacacc gctcgtgagc gaacggcgtg
     3301 ttgccggcga tgtgcggcgt cggtagcacg ttggccgcac ccctgaacca ggcgtcgcgg
     3361 ggcggaggct cgggatcgaa cacgtcgacc gcgacgcgaa gctcctccgc ccgcgcgcgg
     3421 cgccacagcg cctcctgctc gacgacggcc atccggctga ccagcacgaa cagcgacccc
     3481 ggcgccagcg cttcgatgac ctccgcgtcg atgaccccga gggtcgacgg cgtcggcggg
     3541 atcgcgacga cgaagatgtc gctcgaccgt gcgagctcga ccagggagac cgctcgcgtc
     3601 accgagtgcg ccgcggcgac ctcgtcatca acgagcgggt cgaacaccga gaccggacat
     3661 ccatacggct cgacgaattg gcggtagtgc tggttgatgc tgccgaagcc ggcaaggccg
     3721 acctgggagt cggcgaggtc gcggaagagg tacgtgctcc ccgtcttccc cgagtcctgc
     3781 cagccgccgc tgcgtaccag gtcaatggac cgcgggatgt cgcggagcag tgcgaacgtg
     3841 atcgccaggg cgaactcggc cacggtccat gtcatcgtcc gcgacgtgtc gaccaccgtc
     3901 accccacggc gggcggcggc ttcgaggtcg atccagctca accggtagtc gaacgtgcct
     3961 gcgatgacct tcagccgcga cgctcgagcg aggcgggctt catcgaatgt tccgccctcg
     4021 ccgagccaca gccagggtga ggcgaccaag gcgtcggcgt gcgccagacc ggcgtcgagc
     4081 atctcggcac gcgcctcggc cgggtcgtcg accccccatt ccaggcactc gatggcaccg
     4141 tgcggcgaca gcactccgtc ggccaggccc cggtaccgct cgcccccgtg gaagtagcgt
     4201 cgggagctct gcgtcgacca ccacccgtcc tcccgcggcg agacgtagag gaaccgaggc
     4261 cgatccgtca tgtcccggtc tccttctgcg cgtccctggg gcgaagctcc ttgaacctcc
     4321 gcactgtgtc ggtgatcgcg acctcggtcg gaaggcgttc catgctggac gcgccgaaga
     4381 aaccggcgac gccggacgtg cgagccagga cgtacgccgc atcgtctggc tcagagatcg
     4441 gaccgccgtg acagagcacg atcacatcgg ggctcacgtc gacggctgag tcgcgaatct
     4501 cctggatgag gccaacacaa tggtcaaggg tcttgccggt ctcggccccg atgctgccgc
     4561 cggttgtcag gcccatgtgc gcgacaagga cgtccgctcc ggctccagtc atcgcgcgcg
     4621 cgtcgtcggc atcgaacacg tacggcgtgg tcagcaggtc gagggcatgc gcttgtctga
     4681 tcatctcgac ctcgagggcg taccccatgc cggtctcctc gaggttctgg cggaacgtcc
     4741 catcgaccag gccgacagtc gggaagttct ggacgccggc gaacccgatg tccttcagct
     4801 gctggaggaa caccgacata acgcggaacg gatcggttcc acagacaccg gccagcaccg
     4861 gcgtctgccg caccacgggc agcacctcgg ccgccatctc gaccacgatc gcattggcgt
     4921 cgccgtacgg catcaggccg gccagggagc cacgcccggc catccggtac cgaccggagt
     4981 tgtagatgat gatcaggtcg gcgccacccg cctccgcaca cttggcgctc agcccggtgc
     5041 ccgcgccggc gccgacgagc acgccgccgc cgtccagttg cgcgcggaag cggtccaaca
     5101 gcggcgccct catgttctcg aagccgcttt gatcatggcg tccaaccggt tcgccatcgc
     5161 gagagcgaag tcggggtcgt tgacatcgga ttccaactca tggacctcga cgttcgcggc
     5221 caccgtctcg cggatcgacg agaagag
//
LOCUS       DATBFZ010000396         7333 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_932447, whole genome shotgun sequence.
ACCESSION   DATBFZ010000396 DATBFZ010000000
VERSION     DATBFZ010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 7333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7333
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_932447"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..869)
                     /locus_tag="VH419_11945"
     CDS             complement(<1..869)
                     /locus_tag="VH419_11945"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine kinase"
                     /protein_id="HEY7044371.1"
                     /translation="MSVLDARGAATTARFHVVGIGNALVDVIAHHSEAFVVEKALVKG
                     SMTLIEADRALSLHAALVAPIEMSGGSAANTMCGVASFGGRAAYIGKVAPDALGEAFR
                     HDLRAEGVAFPNPAARAEVPTGRCLIVVTPDAERTMNTFLGVSSLLGPDDVSTEVVAA
                     GQVVYMEGYLYDRDEAKAAYRKAAAVAHAAGRKVSLTLSDSFCVDRHRADFLALVHDE
                     VDILFANADELCSLYEVDSLDDGVARVRQDCELAAITTGPEGSMVVTGNDVLKVKAEP
                     VAQVVDTTGAGDL"
     gene            1001..3595
                     /locus_tag="VH419_11950"
     CDS             1001..3595
                     /locus_tag="VH419_11950"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF023266.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEY7044372.1"
                     /translation="MNAPSSDDLHRLPRTARPTRYDLLLEPDLEHATFSGEVAISVDV
                     EQPTDILVLNAAELSIDEATVSGDAPRSCSVELDEDVERLVVTLPEPAVPGPLTLRLR
                     FRGVLNDKLRGWYRSTFRDDDGREQVIATSQMQATDCRRAFPCWDEPEHKAVFAVTMR
                     VSEGLLAISNGPEISREGEGTGTVTVRFADTMPMSSYLVAFVVGPLEATAPVDVDGVP
                     MRVVHVPGKGHLTGFALEVGAFALRWFQRYYGIPYPSDKVDLVALPDFAAGAMENLGC
                     ITFRESVLLVDPASATQAEEQLVADVVAHELAHMWFGDLVTMRWWNGIWLNEAFATFM
                     EVAACDAFRPAWKRWESFSLERTAAFDTDSLLSTRPVEFEVVSPEDADGMFDVLTYQK
                     GGALLRMLEQYLGEDGFRDGIRHYLGKHSYGNTETSDLWDALEATSAEPVRRIMDSWI
                     WQEGYPLVSASISADGEALLLRQRRFVFTDPGGEQDDDDRARRGTETRWAIPIQVSQS
                     LPSGESHESKVLLDADETSVALLDPDAVVVVNCGGHGFYRVAYDTTLRERLTGAAQSL
                     STVERYALVDDAWAAVVAGEASAPDFCALAEGFAAEPDVAVWRTLVSGLGWCDRLLDG
                     DARDRFRAFVRDLVGPRLEALGWRAEPDEDELTGELRGLLIRALAILGDDAAAQQRAA
                     ELFERQLEDASANDPEVAAAVVNVVATTGGPDRYDRFVDCFRTAPTPQEKLRYLYALA
                     DFRDEALMARTLEFLVGDEVKTQNAPFVLGRCIANRDRGEQAWRFVREQWDHANAAFP
                     DNSIVRMIDPVKTLIREDQQADVAAFFAEHEIPQAAKTLQQVLERQRINVALREREAA
                     PLSTYFGA"
     gene            3592..4698
                     /locus_tag="VH419_11955"
     CDS             3592..4698
                     /locus_tag="VH419_11955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEY7044373.1"
                     /translation="MTPTSVEVFAVEPTAAQLIVRSPTDGPHELAVGDGTWTAEVHDG
                     ITAVEVDDLEPYAMYALSLDGQPAGEFRTLPPPPGRYLGRFATVSDLHIGETGFGHVP
                     RFTSDTDYTHAHPVVALRAAVRELAAWGSQGLVVKGDLTHDCRAKEFDLAASILATYP
                     GPLRVMPGNHDGGNHNHDDGVPVLAAHGLELQWATRSAVLGGLNVVMVNTVRRGHEHG
                     YPPSDDDPVFELIRDAPSMVLIHHQLMTTRLPYYVPFGVPKGPARDFLGRLASVNPAV
                     LLSSGHTHRHRRRRYGPIAITEVGSVKDHPGTWAGYLVYEGGVVQVVRRIADSTVLVW
                     TERTRRNAMGAWGRWSPGRLEDRSFSHAWPNGTR"
     gene            complement(4667..5839)
                     /locus_tag="VH419_11960"
     CDS             complement(4667..5839)
                     /locus_tag="VH419_11960"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HEY7044374.1"
                     /translation="MNAGDGQRSDHEQASGPSADATADEGLVKRVSSRVDRQKERAQE
                     ILKEAESRRPKSALLDVGFTAYEHDGLVGGGVLAGAVAFRIFLFVVPLVFVFVSGFGL
                     AADAANRTPSDLAASAGILGLMASMVKTVDDQSFGTRLTIFLVGGFALVIAARTLMLV
                     LNAVFVLVWRLPRTKIRRKLRAVSGLLLIVLLGALMVRLLAWFDDQSVVVAMAGTVVS
                     LALVMVGWTWASLRVFPHPPELLASDVLPGAVLVAFGVEAMRLFTVIYVSRSFEHKSQ
                     TYGAVGGSLALLLWAYVLGRILIGSAVLNAACWQHKQRMLRHWSPPAMPQPSAPVANG
                     PDMPQPAAPVANGPDMPQPSAPVANGPLPPPPPPPLPSPPPPSASVVSESRSARRG"
     gene            complement(5930..6640)
                     /locus_tag="VH419_11965"
     CDS             complement(5930..6640)
                     /locus_tag="VH419_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008363451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY7044375.1"
                     /translation="MTRVLVVDDEPQIRRALSVNLTARGYHVDAAASGEEALRMAASV
                     HPDVVLLDLGLPGIDGTDVVRGLRGWSNVPIIILSVRDSESDKVAALDAGADDYVTKP
                     FGINELLARLRAALRRHQPAAENAVVRTDDFTIDLAAAKAWRGDEEVHLTPTEWELVK
                     HLVRNPGRLLTQRWLLQQVWGPSYSTETAYLRVYLAQLRRKLEPNPSQPRYFVTEPGM
                     GYRFEPGDSVEATGSSPA"
     gene            complement(6637..>7333)
                     /locus_tag="VH419_11970"
     CDS             complement(6637..>7333)
                     /locus_tag="VH419_11970"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY7044376.1"
                     /translation="SHDLRTPLATIKASATSLLQSDVSWDANQRREFAQTIDDEADRL
                     NRLVGNLLDMSRLHAGAVSMTLRPVYLEDVVAAALASVDHDPTRLHVSVPETLPAVYA
                     DPALLERALANVIANALAWSPPGAPVAVEAAEVASRIHLRIIDRGPGVLPRDRDKVFQ
                     PFQRLGDRSTQAGVGLGLAVARGFIEAVGGSIDLDDTPGGGLTVLVDLAEVPAGGPVE
                     PVAPPPSVTSVAT"
BASE COUNT         1124 a         2601 c         2533 g         1075 t
ORIGIN      
        1 tacaggtcgc cggcgccggt ggtgtccacc acttgcgcga cgggttcggc cttcaccttc
       61 agcacgtcgt tccccgtgac gaccatcgag ccttccggtc cggtggtgat ggcggccagc
      121 tcgcagtcct gacgcacgcg agcgacgccg tcgtcgagcg agtcgacctc gtacagcgaa
      181 cacagttcgt ccgcgttggc gaacaggatg tcgacctcgt cgtgcacgag ggcgaggaag
      241 tcggcccggt gacggtcgac gcagaaggag tccgacagcg tcagtgacac cttccggccc
      301 gcggcatggg ccacagcggc ggctttgcga taggccgcct tggcctcatc gcggtcgtac
      361 aggtagccct ccatgtagac gacctgaccc gccgccacca cctcggtgct gacgtcgtct
      421 gggccgagca gcgacgacac accaaggaac gtgttcatcg tccgctcggc gtccggcgtg
      481 acgacgatga ggcagcgccc ggtcggcacc tcggcgcggg ccgccgggtt cgggaaggcc
      541 accccttcag cacggaggtc atggcggaag gcttcgccga gggcgtccgg cgccaccttg
      601 ccgatgtacg ccgctcgtcc gccgaacgac gccacgccgc acatggtgtt cgcggccgag
      661 cctccggaca tctcgatcgg cgcgacgagg gcagcgtgca gcgagagcgc ccgatcggcc
      721 tcgatgaggg tcatcgaccc cttgaccagc gccttctcga cgacaaaggc ctcgctgtgg
      781 tgagcgatga cgtcgacgag cgcgttcccg atcccgacca cgtggaagcg cgctgtcgtg
      841 gcggctcccc gcgcgtcgag cactgacacg ggacgtcacg ctagcggcgc gcggcgcgaa
      901 cgggcgtcga ctgcacggca tcgcgatcac gtcgtccgac agccggggga aggggttacg
      961 acggacgagc ccgcgggatg ggcagatacg ctcgaccgcc gtgaacgcgc cctcgagcga
     1021 cgacttgcat cgcctccccc gcaccgcccg gcccacgcgg tacgacctgc tgctcgagcc
     1081 ggacctcgaa cacgccacct tctcgggcga ggtggcgatc agcgtcgacg tcgagcagcc
     1141 gaccgacatc ctcgtgctca acgcggccga gctgtcgatc gacgaggcca cggtgagcgg
     1201 ggatgcgcct cgctcgtgct cggtcgagct cgacgaggac gtcgagcgtc tcgtcgtgac
     1261 gctgcctgag ccagccgtcc ccgggccgct cacgttgcgg ctccgcttcc gcggcgtcct
     1321 gaacgacaag ctccgcggct ggtaccgctc cacgttccgg gacgacgacg gtcgggaaca
     1381 ggtcatcgcc acctcgcaga tgcaagccac cgactgccgc cgggcctttc cgtgctggga
     1441 cgagcccgag cacaaagcgg tcttcgccgt gaccatgcgg gtgtcggaag ggctgctcgc
     1501 catctcgaac ggacccgaga tctcgcgtga gggcgagggc accggcacgg tcacggttcg
     1561 cttcgccgac acgatgccga tgtcgagcta cctcgtggcc ttcgtcgtcg gaccactcga
     1621 ggcgaccgcg cccgtggacg tcgacggcgt gccgatgcgc gtcgtgcacg tacccggcaa
     1681 gggccatctg accggcttcg cactcgaggt cggcgcgttc gcgctgcggt ggttccagcg
     1741 ctactacggg atcccgtacc cctcggacaa ggtcgatctg gtcgcccttc cggacttcgc
     1801 cgccggggcc atggagaacc tcggctgcat caccttccgg gagagcgtcc tgctcgtcga
     1861 tccggcgtcc gccacgcagg ccgaggagca gctcgtcgcc gatgtcgtcg cccacgagct
     1921 ggcccacatg tggttcggcg acctcgtcac catgcgctgg tggaacggca tctggctcaa
     1981 cgaggcgttc gccaccttca tggaggtcgc ggcgtgcgac gccttccggc cggcgtggaa
     2041 gcgctgggag agcttcagcc tcgagcgcac cgcggcgttc gacacggaca gcctgctcag
     2101 cacgcgtccc gtggagttcg aggtcgtctc gcccgaggac gccgacggca tgttcgacgt
     2161 gctcacctac cagaagggcg gcgcgctgct gcgcatgctc gagcagtacc tcggtgagga
     2221 cggattccgc gacggcatcc gccactatct cggcaagcac agctacggca acaccgagac
     2281 gtcggacctg tgggacgcgc tcgaggccac ctcggccgag ccggtgcggc gcatcatgga
     2341 cagctggatc tggcaggagg gctatccgct cgtcagcgcg tcgatctcgg cggacggcga
     2401 ggccttgctc ctgcgccaac gccgcttcgt gttcaccgac ccgggcgggg agcaagacga
     2461 cgacgatcgg gcgcgacgcg gcaccgagac ccgctgggcg atccccatcc aggtctcgca
     2521 gtccctcccg tcgggcgagt cgcacgagtc gaaggtgctc ctcgacgcgg acgagaccag
     2581 cgtggcgctg ctcgacccgg acgcggtcgt cgtcgtcaac tgcggcggcc atggcttcta
     2641 ccgcgtcgcc tacgacacca ccctccgcga gcgcctgacc ggcgccgcgc aatcgctctc
     2701 gaccgtcgag cgctacgccc tggtcgacga cgcatgggcc gccgtcgtcg ccggcgaggc
     2761 gagcgcgccc gacttctgtg cgctcgccga agggttcgcc gccgagccgg atgtggccgt
     2821 ctggcgcacg ctcgtgtccg gcctgggctg gtgcgatcgc ctgctcgacg gtgacgctcg
     2881 cgaccgcttc cgggccttcg tccgggatct ggtcgggcct cgtctcgagg ccctgggctg
     2941 gcgagccgag cccgatgagg acgagctcac cggcgagctg cgcggcctgc tcatccgcgc
     3001 cctcgccatc ctcggggacg atgccgctgc gcagcagcgg gctgctgagc tcttcgagcg
     3061 ccagctcgag gacgcgtccg ccaacgatcc ggaggtggcc gccgcggtcg tcaacgtcgt
     3121 cgccacgact ggcgggccgg atcgctacga ccgtttcgtc gactgcttcc gcacagcgcc
     3181 gacgccgcag gagaagttgc gctacctcta cgcgctggcc gacttccgcg acgaggccct
     3241 catggcgcgc acactcgagt tcctcgtcgg cgacgaggtg aagacgcaga acgcgccctt
     3301 cgtactcggt cgttgcatcg cgaaccgtga ccggggcgag caggcttggc ggttcgtacg
     3361 cgagcaatgg gaccacgcca acgctgcgtt ccccgacaac tccatcgtgc gcatgatcga
     3421 cccggtgaag acgctgatcc gcgaggacca gcaagccgat gtcgcggcgt tcttcgccga
     3481 gcacgagatc ccgcaggccg cgaagacgct ccagcaggtc ctggagcgtc agcggatcaa
     3541 cgtcgcgctt cgcgagcgcg aggccgcacc gctcagcacc tacttcggcg cgtgacgccg
     3601 acctccgtcg aggtcttcgc ggtcgagccg accgcggccc agctgatcgt ccggtcaccc
     3661 accgacggcc cgcacgagct ggccgtcggt gacggcacct ggacggccga ggtccacgac
     3721 ggcatcaccg cggtggaggt cgacgatctc gagccctatg cgatgtacgc gctcagcctc
     3781 gatgggcaac cggcaggcga gttccgcacc cttccgccgc cgccgggccg gtacttgggt
     3841 cgcttcgcca ccgtgtccga cctccacatc ggcgagaccg gcttcgggca cgtcccccgc
     3901 ttcacgtccg acaccgacta cacgcacgcc catccggtcg tcgccctgcg cgccgccgtc
     3961 cgggagctgg cggcgtgggg ctcgcaaggg ctcgtcgtca agggcgacct cacgcacgac
     4021 tgtcgggcga aggagttcga cctcgcggcc tcgatcctcg ccacctatcc gggtccgctc
     4081 cgggtgatgc cgggcaatca cgatggcggc aaccacaacc acgacgacgg cgttcccgtg
     4141 ctcgccgcgc acggcctcga gctgcaatgg gccacgaggt cggcggtgct cggcgggctg
     4201 aatgtcgtga tggtgaacac ggtccgccgc ggacacgagc acggctaccc acccagcgac
     4261 gacgatccgg tcttcgagct catccgcgac gcaccgtcca tggtgctgat ccatcaccag
     4321 ctgatgacga cgcggctgcc gtactacgtg cccttcggcg tgccgaaggg cccggcccgg
     4381 gacttcctgg ggcggctggc gtcggtcaat cccgcggtcc tcctcagcag cggccacacc
     4441 caccgtcacc gccgccgtcg ctacggtccg atcgcgatca ccgaggtggg ttcagtgaag
     4501 gaccacccgg ggacgtgggc gggctatctc gtgtacgagg gtggtgtggt ccaggtggtc
     4561 cggcgcatcg ccgattcgac cgtgctggtc tggaccgaac gcacgcgccg caacgcgatg
     4621 ggcgcctggg gccggtggtc accaggccgg ctggaggacc gctcgttcag ccacgcctgg
     4681 ccgaacggga ctcgctgacg accgaggccg acggcggcgg aggtgaaggc agcggtggtg
     4741 gcggcggtgg cggcagcgga ccgttcgcga ccggcgcgga cggctgcggc atgtccggac
     4801 cgttcgcaac cggtgccgcc ggctgcggca tgtccggacc gttcgcgacc ggcgcggacg
     4861 gctgcggcat cgccgggggc gaccagtgcc gcagcatccg ctgcttgtgc tgccagcagg
     4921 ccgcgttcag cacggccgag ccgatgagga tccgccccag cacgtaggcc cacagcagca
     4981 gggcgagcga tccgccgacc gcgccgtagg tctgtgactt gtgctcgaac gagcggctca
     5041 cgtagatgac cgtgaacagc cgcatggcct cgacgccgaa ggcgaccagc accgcgcccg
     5101 gcagcacgtc ggaggcgagg agctcgggcg ggtgtgggaa caccctgagc gacgcccacg
     5161 tccatcccac catgaccaac gccagtgaca cgacggtgcc ggccattgcg acgacgacgg
     5221 actggtcgtc gaaccaggcc aacagcctca ccatcaaggc gccgagcaag acgatgagga
     5281 gcagaccgga gacggcgcgg agcttgcggc ggatcttcgt gcgagggagc cgccacacga
     5341 gcacgaacac ggcgttcagc acgagcatca aggtgcgcgc cgcgatgacc agcgcgaagc
     5401 caccgacgag gaagatcgtg agccgagtgc cgaacgactg gtcgtccacg gtcttcacca
     5461 tcgatgccat gagcccgaga atgccggcgc tggccgcgag gtcggacggt gtccgattgg
     5521 cggcgtcggc ggcgaggccg aacccggata cgaagacgaa gaccaacggc accacgaaca
     5581 agaagatcct gaacgccacg gcgccagcca gcacccctcc gccgaccagg ccgtcgtgct
     5641 cgtacgccgt gaagccgacg tcgagcaggg cggacttcgg ccgacgggac tccgcctcct
     5701 tgaggatctc ctgggctcgc tccttctgcc ggtcgacccg ggacgacacc ctcttcacca
     5761 gcccttcgtc cgcggtcgcg tcagccgaag gtccgctcgc ctgctcgtgg tcgctgcgct
     5821 ggccgtcgcc ggcgttcacg ggccgattct gccagccggc ccgtccgggc cgggctcggc
     5881 cacgcgacgg cgttggagca gccggcacgc gcccagtcgg tgcgggcgtt caggccgggg
     5941 acgaccccgt cgcctccacc gagtcgccgg gctcgaagcg gtatcccatc ccaggctccg
     6001 tcacgaagta ccgcggctgg ctggggttgg gctcgagctt ccggcgcagc tgggcgaggt
     6061 agacgcgcag gtaggcggtc tcagtgctgt agctcgggcc ccacacttgt tgcagcagcc
     6121 atcgttgcgt cagcaaccgt cctgggttgc ggacgaggtg cttcacgagc tcccactcgg
     6181 tgggcgtgag gtggacctcc tcgtcaccac gccacgcctt cgccgccgcg aggtcgatcg
     6241 tgaagtcgtc agtgcgcacc accgcgttct cggccgcggg ttgatggcga cgcagcgcgg
     6301 cgcgcagtcg ggccaggagt tcgttgatgc cgaacggctt cgtcacatag tcgtcggcac
     6361 cggcgtcgag cgcggcgacc ttgtcggact cggagtcgcg cacggacagg atgatgatcg
     6421 gcacgttcga ccagccccgc agccctcgca cgacatccgt cccgtcgatg ccgggcaggc
     6481 cgaggtcgag cagcacgacg tcggggtgga ccgacgccgc catgcgcagc gcttcctcac
     6541 ccgacgctgc agcgtcgacg tggtagccgc gggcggtgag gttcaccgac agggcgcgcc
     6601 ggatctgcgg ctcgtcgtcg acgacgagga cccgggtcat gtcgccaccg aggtcaccga
     6661 cggcggcgga gcaaccggtt cgacggggcc accagccggc acctcggcca ggtcgacgag
     6721 gacggtgagt cccccaccag gcgtgtcatc gaggtcgatg ctgccaccca cggcctcgat
     6781 gaagccacgg gcgaccgcca gtccgaggcc cacgccggcc tgggtgctcc ggtcgccgag
     6841 ccgctggaac ggctggaaca ccttgtcgcg atcccggggc agaacgccgg gtccacggtc
     6901 gatgatccgc agatggatcc ggctggccac ctctgcggcc tcgactgcca ctggtgcccc
     6961 gggcggtgac caggccagcg cgttggcgat cacgttggcc agtgcccgct cgaggagggc
     7021 tgggtcggcg tagaccgccg gcagggtctc gggcaccgag acgtgcagcc gggtcgggtc
     7081 gtgatcgacg gatgccaggg ccgccgccac cacgtcctcg aggtagaccg gtcgcagtgt
     7141 catgctgacc gctcctgcgt gcagccggct catgtcaagc aggttgccga cgagacggtt
     7201 caagcggtcc gcctcgtcgt cgatggtctg ggcgaactcg cgccgctggt tggcatccca
     7261 cgagacgtcc gactgcaaca ggctggtggc cgacgccttg atcgtggcga gcggcgtacg
     7321 caggtcgtgg ctc
//
LOCUS       DATBFZ010000397         5819 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_273058, whole genome shotgun sequence.
ACCESSION   DATBFZ010000397 DATBFZ010000000
VERSION     DATBFZ010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 5819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5819
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273058"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            233..676
                     /locus_tag="VH419_11975"
     CDS             233..676
                     /locus_tag="VH419_11975"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008354970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY7044377.1"
                     /translation="MEIFSRSFGEVDPVTAYRLWALRQDVFVVEQQCPYGDLDGLDLE
                     PGTRHVFAVAEGRPVAYLRIVDEPNGTCRIGRVCVMAAHRSAGLAGRLMERALADIGV
                     RDSVLSAQAHLEGWYTRFGYTVTGPVFLDDGIPHVPMRRSAVPVD"
     gene            complement(750..1730)
                     /locus_tag="VH419_11980"
     CDS             complement(750..1730)
                     /locus_tag="VH419_11980"
                     /inference="COORDINATES: protein motif:HMM:NF014080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY7044378.1"
                     /translation="MLDVLGLETTEAAAYRRLVEVPYGTAEDLAAMLGSDAAFAGQLL
                     GSLESKGLVARSPSGHDRFVASPPAIALGALVVQRQEELRSAQLELNELTERYRGAAA
                     ERSVSDVVDVVRGSQAVGQRFAQLQRTAEHDMQMLVKSEFVVVPPEENLDETTALARG
                     VAYRVVVERPHLERPGFFEDAAQSIRSGLQARVVERLPLRMAIADQEIALVPLMSADD
                     KDQGGGALLIHPSGLLDALLSMFDLVWQSAHQLVISADSVGEAEGDALEDVDAQILGL
                     LLAGLTDQAIGNQLGLSLRTVQRRVSGLMERANVATRLQLGHQAARRGWL"
     gene            2054..5602
                     /locus_tag="VH419_11985"
     CDS             2054..5602
                     /locus_tag="VH419_11985"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEY7044379.1"
                     /translation="MHRVTLITGDLVTVTPVAGGGSTVSVNAATRTGEGFRTQTVGDD
                     IYVLPDAVLPYLAANRLDKELFNVTGLIAQGYDDASAKALPLIVTYQQSVKRVATAKA
                     PVGARRTDVLASVDGAAMSASKATAERFWATITPTHVRPTAARFDGGIAKVYLDAKVQ
                     ADLADSVAQIGAPQAWAAGFDGTGSTVAVLDTGIDASHPDVSGQISESESFVPGETVD
                     DYHGHGTHVASTIAGTGAASGGVEKGVAPGADLVIGKVLGNDGFGQESWIIDGMEWAA
                     HHADVVNMSLGSDQPSDGTTPMDQAVDNLSDETGALFVIAAGNNGMVGGVTAPGAADA
                     ALTVGAVDGQDQLAWFSNMGPRLGDSALKPDLVAPGVDILAARSHFVDGTGDYQTLSG
                     TSMATPHVAGAAAILAERHPGWNGQQIKDALMSAAKGLDAYTPYQVGTGRVDVPAALG
                     NVHATGSTSFGFFKWPNDDAEPVDRTVTYTNDGDDPVTLDLSADFRDASGTPAADGVL
                     TMSADQVTVAAHGSATMTLTADPSQVSTGTQLSGTVLASTAGTPVARTSIALDKEAER
                     YNLTLRAKGRDGQPTSTWVTLHAFDDSTLEPVFVDGETTIRLPAGTYSAMTFMDTQDA
                     PDAKALAVLGNPQIDLSHDRAVDLDASKAVPVTVGVGRRAEATATRVEYFSSAGAGLD
                     EALWMPFDIDRIYASPTARVTDGTFDFVMRWRLRKPTFDVSTGTKHFDVSQAPGGAWL
                     HGDSRLRTAYLGTGTAADYAGADVTGKAVVITRDAAVPDRTAAIRARAHGAALLLVAN
                     NAPEEFNDWIYGPNLETVRLPVASVSGLEGPALVRLARQGRPISVSGGLNSPWVYDLQ
                     APYVGRIPHNLAYRPARTDLARIDSTYYGDQHRLGGEFRYDFRPFSPYATGYQLYTGF
                     PQSRVEWVNNPAGAAWYKDATVLDGQWQVRGERELFKPGHRYTEKWFDPVIHPRLGSG
                     YWRPERQSDFVQINLPSFGDSGRGHTGGMDPSDQTMWLYHGKELIKESRGWQSMWADL
                     PARRMKLRVVSDARRPSTWKTSTYTRSEYTFWTEHADPSKPAAILPLVQVNFHVDTDL
                     AGDTKAGRQDTIGFDAWTMADAAMAGRLTGGRFEVSYDGGASWHKVALTGKTGHWKAH
                     LTYPKNPARSVSLRATAWDSKGNRVTQAIISAYGLK"
BASE COUNT          949 a         2026 c         1953 g          891 t
ORIGIN      
        1 cggcgcatcc agcaccgccg cgaactgccg gccccgggac cgccacaacg ccgacgaacc
       61 cggcgccggc acatcccgcc gatccaccgg ctccccgtag acatccaacg accgtacgaa
      121 catgtgttcg attctagacg acccaaccga cagttttccg cccgcgaacc catctctgga
      181 agccgtccct gtctcgcagg aacagcgcca gcccagaaga gatgatgggt cggtggagat
      241 cttcagtcgg tcgttcggcg aggtggatcc ggtcacggcg taccgtctct gggcgctacg
      301 ccaggacgtc ttcgtcgtag agcagcaatg cccgtacggg gacctcgacg gactcgacct
      361 agagcccggg acacggcacg tgttcgcggt cgccgagggc cgcccggtcg cgtacctccg
      421 cattgtcgac gagccgaacg gcacctgccg gatcggccgc gtctgtgtga tggccgcaca
      481 tcgatcagcc gggctcgccg gacgcttgat ggaacgcgcg ctcgccgaca tcggcgtacg
      541 ggacagcgtg ctcagcgcgc aggcacacct cgagggctgg tacacccgct tcgggtacac
      601 cgtgacaggc ccggtgttcc tcgacgacgg catcccgcac gtccccatgc gaaggtctgc
      661 cgtgcccgtt gactgagctt gtcgggaccc gctgatcgag cttgtcgaga tcgagggttc
      721 cgggcccgac agatcgaccg cgggatgggc tagagccagc cgcggcgagc ggcctggtgg
      781 ccgagctgga ggcgcgttgc gacgttggcg cgttccatga ggccactgac acgacgttgc
      841 acggtgcgca gggagaggcc gagctggttg ccgatggcct ggtcggtcaa cccggccagc
      901 aggagaccca ggatctgagc gtcgacgtcc tcgagcgcgt ccccctcggc ctcaccgacg
      961 ctgtccgccg agatcaccag ttggtgcgcg ctctgccaga cgaggtcgaa catgctcagc
     1021 agggcatcga ggagtccgct cgggtggatc aggagcgcgc cgcctccctg gtccttgtcg
     1081 tcggcggaca tcaagggcac gagcgcgatc tcctggtcgg cgatcgccat ccgaagcggg
     1141 aggcgctcga cgacccgagc ctgcaagccg ctccggatcg actgcgcagc atcctcgaag
     1201 aaccctggtc gctccagatg gggacgctcg acgacgacgc gataggccac cccacgagca
     1261 agcgccgtcg tctcgtcgag gttctcctcc ggcggcacga cgacgaactc ggacttcacc
     1321 agcatctgca tgtcgtgctc ggccgtccgc tgcagctggg cgaaccgctg accgaccgcc
     1381 tgtgaaccgc ggaccacgtc gaccacgtcg ctcaccgacc gctcggccgc ggcgccccgg
     1441 taccgctcgg tgagctcgtt cagctcgagc tgggcgctgc gcagctcctc ctgtcgctgc
     1501 accaccaagg cgccgagggc gatggccggt ggtgacgcga cgaaccgatc gtgcccgctg
     1561 ggcgaacgag ccacgagtcc cttggattcc agcgagccga gcagctggcc cgcgaatgcc
     1621 gcatcggagc cgagcatcgc ggccaggtcc tcggcggtgc cgtagggcac ctcgacgagt
     1681 cgtcggtagg cggccgcctc cgttgtttcg agtccaagca cgtcgagcat ggtggcgcct
     1741 cctcgcccgg attgacctgc tacgccgtgg cggatagccg ccatgacgta tttccgccgt
     1801 tgccctggtc gatcctacgt ggcactttcg tgtcgctatc ttcctccgcg tcgaaggaca
     1861 ccccatgccc cgtccccccc gccggcccag caccaggtca gccgctctgg tcggaagcct
     1921 cctgatcgga ctgacggcgt ccttggccgc tgtcggtcta cccgccactg cgacgcccgc
     1981 atcgatgtcg tttcccccca cgacgtctgc tgggccgtcc attggccgat cggacattgg
     2041 ccagtcgtct caggtgcacc gggtcacgct gatcaccggc gacctcgtca cagtgacgcc
     2101 ggtggccggc ggggggagca cggtctcggt gaacgcggcc acccggaccg gcgaaggctt
     2161 ccggacccag accgtgggcg acgacatcta cgtcctgccc gacgccgtgc tgccctacct
     2221 cgccgcgaat cgcctcgaca aggagctgtt caacgtcacc ggtctgatcg cccaggggta
     2281 cgacgacgcg agcgccaaag cgctaccgct gatcgtcacg taccagcagt cggtcaagcg
     2341 ggttgccacc gcgaaggcgc cggtcggtgc tcgccgtacc gacgtcctcg cgagtgtgga
     2401 tggtgccgcg atgtcggcgt cgaaggcgac tgctgagcgg ttctgggcga cgatcacgcc
     2461 gactcatgtc cgtccaaccg ctgcccgctt cgacggcggc atcgcgaagg tctacctcga
     2521 cgccaaggtg caggcggacc tcgccgactc cgtcgcgcag atcggagcac cgcaggcatg
     2581 ggccgcgggt ttcgacggca caggctccac ggtggccgtc ctcgacaccg gaatcgacgc
     2641 ctcgcaccct gacgtcagcg gacagatcag cgagtcggag agcttcgtgc ccggcgagac
     2701 ggtcgacgac taccacggtc acggaaccca cgtcgcttcg accatcgccg gtaccggtgc
     2761 agcgtccggt ggtgtcgaga agggcgtcgc gcccggcgca gacctcgtca tcggcaaggt
     2821 gctcggcaac gacggcttcg gtcaggagtc ctggatcatc gacgggatgg agtgggctgc
     2881 ccaccacgcc gacgtcgtca acatgagcct gggatcggac cagccgagcg acggcacgac
     2941 gccgatggac caggccgtcg acaacctcag cgacgagacc ggtgcgctgt tcgtgatcgc
     3001 cgcgggcaac aacggcatgg tcggcggagt gaccgctccc ggcgctgcgg atgcggcgct
     3061 gacggtgggc gccgtcgatg gtcaggacca gctggcctgg ttctccaaca tggggccgcg
     3121 gctcggtgac tcggccttga agcccgacct cgtcgctccc ggcgtggaca tcctggccgc
     3181 acgctcgcac ttcgtcgacg gcaccggtga ctaccagacg ttgagcggta cgtcgatggc
     3241 gacaccgcat gtcgccggcg cagccgccat cctcgccgaa cggcatcccg gctggaacgg
     3301 ccagcagatc aaggacgcac tgatgagtgc ggccaagggt ctggacgcgt acacgccgta
     3361 ccaggtcgga acgggtcgcg tcgacgtccc ggcagcgctt gggaacgtcc atgccaccgg
     3421 ctcgacatcc ttcggcttct tcaagtggcc gaacgacgac gccgagcccg tcgaccggac
     3481 cgtcacctac accaacgacg gcgatgaccc ggtcacgctg gacctctcgg cggacttccg
     3541 tgacgcgtcg ggcacaccgg ctgcggacgg cgtactcacg atgtccgccg accaggtcac
     3601 cgtggctgcg cacggctcgg cgacgatgac gctgaccgcc gacccgtccc aggtgtccac
     3661 aggtacccag ctgtccggaa ccgtcctcgc gagcacggcg ggtacgccgg tcgcccggac
     3721 gagcatcgcg ctggacaagg aagccgagcg ttacaacctg acgttgcgcg cgaagggccg
     3781 cgacggccaa ccgacatcca cctgggtgac gttgcatgcc ttcgacgact cgacgctcga
     3841 gcccgtcttc gtcgacggtg agaccacgat ccgcctgccg gctggaacct acagcgcgat
     3901 gacgttcatg gacacgcagg acgcacccga cgccaaagcg ctcgccgtgc tgggcaaccc
     3961 gcagatcgac ctgtcacacg atcgtgccgt cgacctcgac gcgagcaagg ccgtgccggt
     4021 cacggtcggg gtcggtcgcc gggccgaggc cacggcaacc agggtggagt acttctcgtc
     4081 ggccggtgcc ggcctcgacg aggcgctgtg gatgccgttc gacatcgacc ggatctacgc
     4141 gagcccgacc gcgcgggtca ccgacggcac cttcgacttc gtcatgcggt ggcggttgcg
     4201 taagccgacg ttcgacgtgt ccacgggcac gaagcacttc gacgtgagcc aggcgccggg
     4261 tggtgcctgg ctgcatggcg actcgcgcct tcgaacggcg tacctgggca ccgggacggc
     4321 cgccgactac gcgggcgccg acgtcacggg caaggcggtc gtcatcaccc gggacgcagc
     4381 ggtgccggac cgtaccgccg ccatccgggc gcgcgcccat ggcgccgcgt tgctgctcgt
     4441 ggcgaacaac gctcccgaag agttcaacga ctggatctac gggccgaact tggagaccgt
     4501 ccggcttccg gtcgcctccg tgagtgggct ggagggaccg gcgctcgtcc ggctggctcg
     4561 ccaaggtcgc ccgatctcgg tcagtggtgg cctgaactca ccgtgggtgt acgacctgca
     4621 ggcgccgtac gtcggtcgga tcccgcacaa cctggcgtac cggccggcgc gaacggacct
     4681 ggcacgcatc gactcgacgt actacgggga ccagcaccgg ctcggcggcg agtttcgcta
     4741 cgacttccgc ccgttctcgc cgtacgcgac cggctaccag ctctacacag gcttcccgca
     4801 gagtcgcgtc gaatgggtca acaacccggc cggggctgcc tggtacaagg acgccaccgt
     4861 gctcgacggc cagtggcagg tccgtggcga gagggagctg ttcaagcccg gtcaccgata
     4921 caccgagaag tggttcgatc cggtcatcca cccgcggctc gggtcgggct attggaggcc
     4981 ggagcggcag agcgacttcg tccagatcaa cctgccgtcg ttcggtgact ccggtcgcgg
     5041 acacaccggc gggatggacc cgagcgacca gacgatgtgg ctgtaccacg gcaaggagct
     5101 gatcaaggag agtcggggct ggcagtcgat gtgggccgac cttccggcgc gacgcatgaa
     5161 gctgcgtgtc gtcagcgatg ctcggcgccc ctccacctgg aagacgtcga cgtacacccg
     5221 gtcggagtac acgttctgga ccgagcacgc cgacccgtcg aagcccgcag cgatccttcc
     5281 gctcgtgcag gtcaacttcc acgtcgacac cgatctagcc ggtgacacga aggcgggtcg
     5341 gcaggacacg atcggcttcg acgcgtggac gatggccgat gccgcgatgg cgggtcgact
     5401 gaccggaggt cggttcgagg tgtcgtacga cggcggggcg tcctggcaca aggtggcgct
     5461 caccggcaag accggtcact ggaaggcgca cctgacctac ccgaagaacc cggctcgctc
     5521 cgtgtcgctg cgcgcgactg cctgggacag caagggcaac cgcgtcacgc aggcgatcat
     5581 ctcggcctac ggcctgaagt agcgaggcgg cttccacggc gttctgtccc acccccctcg
     5641 tctccggcgg ctgctccctg tggagcagtc gccggagaca tgccagtcgc actcggcagg
     5701 cttctgcggt cggcgagatg gtcacggcgg gtcggcgtgg gcgtccgtcg taccgtcgtc
     5761 atcgattgag gtgggatggc ggcgaagagc gggaaccgtt cgtgcccgct gagccggtc
//
LOCUS       DATBFZ010000398         1525 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_1587082, whole genome shotgun sequence.
ACCESSION   DATBFZ010000398 DATBFZ010000000
VERSION     DATBFZ010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1587082"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="VH419_11990"
     CDS             complement(<1..494)
                     /locus_tag="VH419_11990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044380.1"
                     /translation="MKRLMLLGLLLATALLIQPAASFATWLASSSSAGSATATSVSQL
                     GAPTVSRSGSNAVVSWSQGVLGNGVIARGYVVKRTVGSTTTTICTTSEPTRTCTDSAP
                     NPTNASYVVQATYRNWVGPASDATSFTFDNVAPTTTLSTNPTPNSNGWVTSASTVTLT
                     ATDAS"
     gene            complement(491..739)
                     /locus_tag="VH419_11995"
     CDS             complement(491..739)
                     /locus_tag="VH419_11995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044381.1"
                     /translation="MTYTNSEPFSVRVATVVVDTSGTRKCGRKYFVTGTYPQAASVQI
                     RGRSSVSTTVPFGMKLSAPDACQGVSVRVDVTATAVKR"
     gene            917..1081
                     /locus_tag="VH419_12000"
     CDS             917..1081
                     /locus_tag="VH419_12000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044382.1"
                     /translation="MATDTCVTVAGAVLPVREGVPTVRSRRVRRRADAADLRVADPDE
                     RVGAALVGRG"
     gene            complement(1232..>1525)
                     /locus_tag="VH419_12005"
     CDS             complement(1232..>1525)
                     /locus_tag="VH419_12005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044383.1"
                     /translation="YPTGSKTVSVQVKNNNAYPVTLDGASVAHLAVQGSPVGCTIHDV
                     TATVPTAPSTRIAPGSTATVADVVSVAMGSDGDNGCQGKTFTFDLTVTGHSSN"
BASE COUNT          228 a          465 c          582 g          250 t
ORIGIN      
        1 gacgcgtcgg tggcggtcag tgtcaccgtc gaagcggacg tgacccaccc gttggagttc
       61 ggtgtcgggt tggtggagag cgtcgtcgtc ggcgccacgt tgtcgaacgt gaacgacgtg
      121 gcatcgctgg ccggacccac ccagttgcgg taggtcgcct ggacgacata gctggcgttg
      181 gtcgggttcg gtgctgagtc cgtgcacgtg cgggtgggct ccgacgtcgt gcagatcgtc
      241 gtggtggtcg agccgacggt tcgtttcacg acgtagccgc gcgcgatgac gccgttgccg
      301 aggacgccct gcgaccagct gaccacggca ttgctcccgc tacgggacac ggtgggggct
      361 cccagctggg agacgctcgt ggcggtggcc gacccggcgc tgctcgagct cgccaaccac
      421 gtcgcgaagc tcgcagccgg ctggatgagc agcgcggtcg cgagcagcag tccgaggagc
      481 atcaggcgct tcatcgcttc accgccgtgg cggtgacgtc gacgcggacc gagacaccct
      541 ggcaggcgtc cggcgcggac agcttcatgc cgaacggcac ggtcgtcgag accgaggaac
      601 gtccgcggat ctgcaccgat gccgcctggg ggtaggtgcc ggtcacgaag tacttgcgac
      661 cgcacttccg ggtgccgctc gtgtcgacga cgacggtcgc gacgcgcacg gagaacggtt
      721 cactgttggt gtaggtcacc gggagagccg cagtgagacc cggccagagt ccggtcaggc
      781 gtccgacctg gacaccgagg cggttgttcg caggggggat cgggctggtc gtcggtgggg
      841 tcggcttcgg ctggtgtccg tggcctgacc cgtggtgggg gcgtggacgg ccctggtggt
      901 gtcgaccatt tgctgcatgg ccaccgacac ctgcgtgacc gtggccgggg ccgtgctgcc
      961 ggtgcgggaa ggggtgccga ccgtgcggtc gcgccgagta cgccggcgag ccgatgctgc
     1021 cgatctccgc gtggccgacc ccgatgaacg tgtcggagct gctctggtgg gacgaggctg
     1081 acggaccgaa cgcgacgtag gcaccagcaa ctcctccgaa gccgccgagg gccaggagga
     1141 gcccaaggag gaggagatgc gcacgtcgtc gttcggtcac gtcaggtcta tcccgtcgtc
     1201 aaaccagtca gcccagtcag cccagcacgg ctcagttgga gctgtggccg gtgacggtca
     1261 ggtcgaaggt gaaggtcttg ccctggcagc cgttgtcgcc gtcagagccc atcgcaacgg
     1321 agacgacgtc ggcaaccgtg gccgtgcttc cgggcgcgat gcgggtgctg ggcgcggtcg
     1381 gcacagtggc ggtcacgtcg tggatggtgc aacccacggg cgaaccctga acggcaaggt
     1441 gcgcgaccga ggcgccgtcg agcgtgacgg ggtacgcgtt gttgttcttg acctggaccg
     1501 agacggtctt cgaaccggtc gggta
//
LOCUS       DATBFZ010000399         5514 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_4211348, whole genome shotgun sequence.
ACCESSION   DATBFZ010000399 DATBFZ010000000
VERSION     DATBFZ010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 5514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5514
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4211348"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="VH419_12010"
     CDS             <1..516
                     /locus_tag="VH419_12010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HEY7044384.1"
                     /translation="VALMSPAALDRVVTVNESGRWIPAFLDLQTAVDNSRLDQTYNTP
                     SLATIFLLAEQVDWLNGLGGLSAAVDRTTESSSALYAWAEKSEYATPFVADPADRSLV
                     VGTIDFDESVDAAAVAKVLRANGVVDVEPYRKLGRNQLRVAMFPAIEAADVQALTACV
                     DWTIASLAAPH"
     gene            complement(559..1554)
                     /locus_tag="VH419_12015"
     CDS             complement(559..1554)
                     /locus_tag="VH419_12015"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017933414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03557 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEY7044385.1"
                     /translation="MTENTAPRFGYTLMTEQSGPRELVAYAARAEQAGFDFEVMSDHY
                     FPWLDEQGHAAYAWSILGAVTQVTDRVELMTYVTCPTMRYHPAVVAQKAATIGVLSEE
                     RFTLGLGAGENLNEHVIGAGWPPANERHEMLGEAIDIISALFDGGYVDYVGEHYRVDS
                     AKLWDLPTARVPIAAAVSGSQSINLVGPKVDHMVAVEPNVDLVAEWDKVSPNSGRKIG
                     QIPICWDPDRDKAVKRAHEQFRWFGGGWKVNAELPGPAGFAGASQFVRTEDVADSIAC
                     GPDIGAIVGAVKPFVDAGFTDVAIVQVGDQGQSEFLDMAERELVSALRSAYASNT"
     gene            complement(1671..2708)
                     /locus_tag="VH419_12020"
     CDS             complement(1671..2708)
                     /locus_tag="VH419_12020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044386.1"
                     /translation="MPGAWLTGVLVALSCALVGLQGVQPAYSAELDRPRRVFTISSPD
                     ITESSSLAVSTVHPGLVYTTNDSGDSDTVYVLDASTGAVVGRTTLAGVAPLDVEAIAA
                     GSDGSLIVADIGDNDADHSSADLYRIEQPGRGDRSVSPQRVDLTYPGGPRDAESVLYD
                     ASTGRAFVVSKLLRGAKIYATPPHVFKHGNARLRPVAVAPSVATDATFLPGRHFAVIR
                     TYFDATVYTYPGWKRVTTFPLPLQQQGESITAPRSGESVWVGSEGTRSQVLAVPLPAL
                     TEPSPASPTASAGPGSATSSAGDSTSSTSPSQDSERVVTRIFVASLIGLGLLVVVAVV
                     LSIRARRHPSR"
     gene            complement(2727..3962)
                     /locus_tag="VH419_12025"
     CDS             complement(2727..3962)
                     /locus_tag="VH419_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020574315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEY7044387.1"
                     /translation="MTAGSGSDELEHVRTAVLDSDELVRAVVSGRRRNASVPYRRVEL
                     RYVDVRAGRLLQLTAYDETQAHTWNLEPADAVAAVDELLATAYANWYVETRAERWQLR
                     VTKKGRWLAHRAARTATAEVDRSHDRTKMRRLDESDPLFALLGVTTGDGRVKPSRMAK
                     FRQVQDLLAALDPLVESAVTLGPGPDLSADRPLRMVDLGCGNAYLTFAAFRYLTVVRG
                     LPVRGVGVDVKAQARAHNSEIAARLGVADQLTFVEGSIAAAVVDDPPDLVMALHACDT
                     ATDDALARAVLWRAPVIVAAPCCHHDIQRQLDTAVIPDPYPLVTRHGILRERLADVLT
                     DSLRATILRLVGYRVEVIEFVDSKHTPRNALIRAVRTGAPADRSTADAYRSLVDGWHV
                     QPALASLLEPAYPVLSRTA"
     gene            complement(3959..4090)
                     /locus_tag="VH419_12030"
     CDS             complement(3959..4090)
                     /locus_tag="VH419_12030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044388.1"
                     /translation="MRRLTAVVAGAGTLRRMHPRYRRLIIPGALVILLLIVVVAALR"
     gene            complement(4078..4827)
                     /locus_tag="VH419_12035"
     CDS             complement(4078..4827)
                     /locus_tag="VH419_12035"
                     /inference="COORDINATES: protein motif:HMM:NF023144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HEY7044389.1"
                     /translation="MTTHLVPARYFELIDKDTDRLIEMGERGLKEPVPACPGWDVAEV
                     VWHVANVYEHKVRVMADNAWPDPWPPTWEFADDEELAFLRSAKTHLFEEFSRHEVTEQ
                     TQTFGEDTTVGFWVRRMACEIAVHRTDGEQAHTDPTPIPDDLSLDGIDEMLRVMLAGP
                     WWADRVQTDHPVEALVAVESGGRRWLCDVREKAITVTDDAATEAAATVSGRPMDVFLW
                     LWGRAGDDSVTFTGDVGTAHEFRARLVECAG"
     gene            complement(4824..>5514)
                     /locus_tag="VH419_12040"
     CDS             complement(4824..>5514)
                     /locus_tag="VH419_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020391839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY7044390.1"
                     /translation="YSLRRMNPAALHTSPPVARAKGAVRDGIRYVRARPDLVFVFIVA
                     FFAGTFGMNFQMTSALMATGVFHKGAGEYGILGTFMAIGSLTGALLAARRGRPRRMLL
                     VTATMAFAVVEIAAGLMPTYWMFALLLPLLGICAMTMLNAMQTTVQLTAESHMRGRVM
                     ALYMMILMGGTPVGAPVIGWVGETFGARWTLIAGGGVTLVAVVVATLWVMRKQQIDVR
                     ELSLHAAKMAA"
BASE COUNT          923 a         1918 c         1843 g          830 t
ORIGIN      
        1 gtggcgctga tgtcgccggc ggcactggat cgggtcgtga ccgtgaacga gtccggccgc
       61 tggatcccgg cgttcctcga tctgcagacg gcggtcgaca actcccggct cgaccagacg
      121 tacaacaccc cgtcgctcgc caccatcttc ctcctggcag aacaggtcga ctggctcaac
      181 ggactgggtg gcctcagcgc ggcggtggac cggacaaccg agtcgtcgag cgcgttgtac
      241 gcctgggcgg agaagtcgga gtacgcgaca ccattcgtcg ccgatcccgc cgatcggtcg
      301 ctggtcgtcg ggacgatcga cttcgacgag tcggtcgacg cggcggcagt cgcaaaggtg
      361 ctccgggcca acggcgtcgt ggatgtcgag ccgtaccgca agctcggccg caaccagctc
      421 cgcgtcgcga tgttccccgc catcgaggcc gccgacgtac aggccctcac cgcctgcgtc
      481 gactggacca tcgcctccct cgccgcccct cactgacctt ggtgcgtctg tcgggccaca
      541 cgaaggggac cggctgacct atgtgttcga ggcgtacgcc gaacggagcg ccgagacaag
      601 ttcgcgctcg gccatgtcga ggaactccga ctggccctga tcgccgacct gcacgatcgc
      661 cacgtcggtg aagccggcgt cgacgaaggg cttgaccgcg ccgacgatcg caccgatgtc
      721 cggtccgcag gcgatcgaat ccgccacgtc ctccgtccgc acgaactggc tcgccccggc
      781 gaagccggcc ggtcccggga gctcagcgtt caccttccac ccgccaccga accaccggaa
      841 ctgctcgtgg gcgcgcttca ccgccttgtc ccgatcgggg tcccagcaga tgggaatctg
      901 accgatcttc cgacccgagt tcgggctgac cttgtcccac tcggcgacca ggtcgacgtt
      961 cggctcgacc gcgaccatgt ggtccacctt cggcccgacg aggttgatcg actggctgcc
     1021 ggatacggcc gctgcgatcg gcacccgcgc ggtcggaagg tcccacagct tggccgagtc
     1081 aacccggtag tgctcaccga cgtagtcgac gtacccgcca tcgaacagcg ccgagatgat
     1141 gtcgatcgcc tctcccagca tctcgtgccg ctcgttcgcc ggtggccatc ccgcaccgat
     1201 cacatgctcg ttgaggttct cccccgcgcc gagcccgagc gtgaaccgct cctcgctcag
     1261 caccccgatc gtggccgcct tttgcgcgac gacggcaggg tggtatcgca tggtcgggca
     1321 ggtcacgtac gtcatcagct ccacacgatc ggtgacctgg gtgaccgcgc cgaggatgct
     1381 ccacgcgtac gccgcatggc cctgctcgtc gagccacggg aagtagtggt cgctcatcac
     1441 ctcgaagtcg aagccggcct gctcggcccg tgcggcgtac gccaccagct ccctgggtcc
     1501 cgactgctcc gtcatcagcg tgtagccgaa gcgcggtgcg gtgttctccg tcatactcaa
     1561 ccttccgtcg ccgtcggcag cgctgtaccc ggttgatcga caccgataac cgccacaact
     1621 cacggttcga cgaaccacaa ctcacggttc gacgaaccac aactcacggt tcaacgagag
     1681 gggtggcggc gggcgcggat ggacaggacg acagcgacga cgacgagcag gccgaggccg
     1741 atgagggagg cgacgaagat ccgtgtcacc acgcgctcgg agtcctgcga cggggaggtc
     1801 gaggacgtgc tgtccccggc ggaactcgtg gcggaaccag ggccagcgga cgccgtcggc
     1861 gacgcgggtg acggctcagt cagcgcaggc agcggcacgg ccagcacctg tgagcgggtg
     1921 ccctcgctgc cgacccatac ggactcgccg gaccgcggcg cggtgatcga ctcgccctgc
     1981 tgctgcagcg ggagtgggaa ggtcgtgacg cgtttccaac cgggatacgt gtagaccgtg
     2041 gcgtcgaagt acgtccggat caccgcgaag tgacggccgg gcaggaacgt cgcgtccgtc
     2101 gcgacagacg gcgccactgc gaccggtctg aggcgggcgt tgccgtgctt gaacacatgc
     2161 ggtggcgtcg catagatctt ggcgccccgg agcagcttgc tgacgacgaa agcacggccc
     2221 gtcgacgcgt cgtacagcac cgactccgca tcacgtggac cacccggata ggtcaggtcg
     2281 acacgttgcg gcgacacgct ccggtcaccg cggccgggct gttcgatgcg gtacaggtcg
     2341 gcactcgagt gatcggcgtc gttgtctccg atgtcggcca cgatgagcga gccgtcggag
     2401 cccgccgcga ttgcctcgac gtccagcggt gcgacaccgg ccagggtcgt acgtccgacg
     2461 acggcgccgg tcgacgcgtc cagcacgtac accgtgtctg agtcacctga gtcgttcgtc
     2521 gtgtagacga gcccgggatg aaccgtgctg acggccagcg agctggactc ggtgatgtcc
     2581 ggtgaggaga tggtgaagac gcgacgcggc cggtcgagct ccgcggagta cgccggttgg
     2641 acgccttgca gaccgacgag cgcgcacgac aaggccacca gcacacccgt cagccacgca
     2701 cctggcatca tcaccctcgc caccggtcag gctgtccgac tcagcaccgg gtacgccggc
     2761 tccagcaacg acgccagcgc cggctgcacg tgccagccgt cgaccagcga gcggtaggcg
     2821 tcagcggtcg atcggtcagc aggcgctccc gtccggacgg cacggatcaa cgcgttgcgc
     2881 ggggtgtgct tggagtcgac gaactcgatc acctcgaccc ggtaaccgac gagccgcagg
     2941 atggtggccc gaaggctgtc ggtgaggacg tcggcaagcc gctcgcgcaa gatgccatgc
     3001 cgcgtgacga gcgggtaggg atccgggatg acggcggtgt cgagctgtcg ctggatgtcg
     3061 tggtgacaac acggcgcggc gacgatcacc ggcgcccgcc acaggacggc gcgggcgagc
     3121 gcgtcgtcgg tggccgtgtc gcaggcgtgc aacgccatga ccagatctgg cgggtcatcg
     3181 acgaccgcag cagcgatgct cccctccacg aatgtcagtt gatcggcgac accgagacgt
     3241 gccgcgatct cgctgttgtg cgcgcgcgcc tgcgccttca catcgacgcc caccccacgc
     3301 accggaaggc cccgcacaac ggtcaagtaa cggaacgcgg cgaacgtcag gtaggcgttg
     3361 ccgcaaccca ggtcgaccat gcgcagcgga cgatcggccg acaggtcggg gccgggaccc
     3421 agcgtgacgg cggactcgac gagcgggtcg agagcggcga gcaggtcctg cacctggcgg
     3481 aacttcgcca tccggctcgg cttcactcgt ccgtctcccg tggtcacgcc gagcagcgcg
     3541 aacagcgggt ccgactcgtc gaggcgacgc atcttcgtcc ggtcgtggga gcggtcgacc
     3601 tcggccgtcg cggtgcgcgc tgcacgatgc gccagccagc gtcccttctt cgtgactcgc
     3661 agctgccacc gctcggcacg tgtctcgacg taccagttcg cgtacgccgt cgcgagcagc
     3721 tcgtcgaccg ccgccacggc gtctgccggc tcgaggttcc acgtgtgtgc ctgcgtctcg
     3781 tcgtacgccg tcagctggag gaggcggccc gccctcacgt cgacgtagcg aagctcgacg
     3841 cgccggtacg gaaccgaggc gttgcgccgg cgtccggaga ccacggctcg tacgagctcg
     3901 tcggagtcca gcaccgcggt gcggacgtgc tcgagctcgt ccgagccgga cccagcggtc
     3961 atcgcagcgc cgccacgacg acgatgagca gcaggatcac cagcgcaccc gggatgatca
     4021 gccggcggta gcgcggatgc atgcggcgaa gggtacctgc tccagcgaca accgccgtca
     4081 gccggcgcac tccaccaggc gggcccggaa ctcgtgggcg gtgcccacat ccccggtgaa
     4141 tgtcaccgaa tcgtcaccgg ctcgtcccca caaccacaag aacacgtcca tcggccgacc
     4201 ggacaccgtg gcggcagcct ccgtcgcagc gtcgtcagtg acggtgatcg ccttctcgcg
     4261 aacgtcgcag agccaccggc ggccacccga ctcgaccgcc acgagagcct cgacgggatg
     4321 gtcggtctgg acccggtcgg cccaccaggg cccggccagc atgacgcgga gcatctcgtc
     4381 gatgccgtca aggctgaggt cgtcagggat gggtgtcggg tcggtgtggg cctgctcgcc
     4441 gtcggtgcga tggacggcga tctcgcaggc catccggcgc acccagaagc ccaccgtggt
     4501 gtcctcgccg aacgtctgcg tctgctcggt gacctcgtgg cgcgagaact cctcgaacag
     4561 atgtgtcttg gcagacctca ggaaggcgag ctcctcgtca tcggcgaact cccaggtcgg
     4621 cggccacggg tccggccagg cgttgtcggc catcacgcgg accttgtgct cgtagacatt
     4681 ggcgacgtgc cagaccacct cggccacgtc ccagcccgga cacgcgggca ccggctcctt
     4741 cagaccgcgc tcgcccatct cgatgagccg gtcggtgtcc ttgtcgatga gctcgaaata
     4801 gcgtgcgggg accaggtgcg tcgtcatgcg gccatcttgg cagcatgcag agacagctct
     4861 cgtacgtcga tctgctgctt gcgcatcacc cacagcgtcg cgacgacgac cgcgaccaac
     4921 gtcacaccgc caccggcgat gagcgtccag cgggcaccga acgtctcgcc gacccatccg
     4981 atgaccggcg caccgaccgg cgtaccgccc atcaggatca tcatgtagag cgccatcact
     5041 cgcccacgca tgtgcgactc cgcggtgagc tgcacggtgg tctgcatcgc gttcagcatc
     5101 gtcatcgcac agatgccgag cagtggaagc agcagtgcga acatccagta cgtcggcatc
     5161 aggccggcgg cgatctcgac gacggcgaag gccatcgtgg ccgtgacgag gagcattcgc
     5221 cgtggccgtc cacgccgggc ggcgagcagc gcgccggtca gtgaaccgat ggccatgaac
     5281 gtcccgagga tgccgtactc gccggcgccc ttgtggaaga cgccggtggc catgagcgcg
     5341 ctggtcatct ggaagttcat gccgaacgtg cccgcgaaga acgcgacgat gaacacgaag
     5401 acgaggtccg gccgggctcg cacgtatcgg atcccgtccc gtacggcgcc tttcgcccgc
     5461 gccaccgggg gagaggtgtg cagagcggcc gggttcatcc gtcgcagcga gtag
//
LOCUS       DATBFZ010000400         2965 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_5066254, whole genome shotgun sequence.
ACCESSION   DATBFZ010000400 DATBFZ010000000
VERSION     DATBFZ010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2965
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5066254"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="VH419_12045"
     CDS             <1..259
                     /locus_tag="VH419_12045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044391.1"
                     /translation="GADDHALAAKLTIPTLVAVATNDPHCPLGDVQSLYDAVAARTKQ
                     LSILDQNPGRHGWDLLLEAGAPLPLATIVADWITHAGPRRP"
     gene            complement(270..488)
                     /locus_tag="VH419_12050"
     CDS             complement(270..488)
                     /locus_tag="VH419_12050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044392.1"
                     /translation="MNNDVGDMFVEADVVPERRPATMVTALRIDLDIRAELETAAAAR
                     GIGVSTLMREIIEEWARDNGPMPPARTG"
     gene            complement(612..1175)
                     /locus_tag="VH419_12055"
     CDS             complement(612..1175)
                     /locus_tag="VH419_12055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6036 family nucleotidyltransferase"
                     /protein_id="HEY7044393.1"
                     /translation="MRRDQLEHAIRTACQVIERPAVIVVGSQAILGTYTEDQLPPEAT
                     MSVEVDILPIAESNEEAARLADKIEGVAGEWSPFEGQHGFSIDGVDEDTAVLPGGWRD
                     RLVKVQNANTAAPSGRPQYIGWCLDKEDICVAKLCAFREKDQNFVTALLDAGLVGARL
                     IATRLADVSPSHRPAAERALTWLAARR"
     gene            complement(1175..1693)
                     /locus_tag="VH419_12060"
     CDS             complement(1175..1693)
                     /locus_tag="VH419_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY7044394.1"
                     /translation="MPSRGEVLREVMRETGTTQSELSRVSGVRQPSISQFLSGRTAMS
                     DDMLDRLLSCMGYVLEVVRRPVKHQLGRSPERSWRLHRRLSMDLTPESFDHWREPMLR
                     NIRRLRASMRGQPHLRNLDRWQRLIEEGDISGLRRVMTGLDSDSIQMREVSPMGGLLP
                     QHDRSQVLRNAS"
     gene            1836..>2965
                     /locus_tag="VH419_12065"
     CDS             1836..>2965
                     /locus_tag="VH419_12065"
                     /inference="COORDINATES: protein motif:HMM:NF017885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEY7044395.1"
                     /translation="MPLGEQLAAARARSGYDQDDVANGLGVSRAMVSYWESGRRTPND
                     RQMVALAQLLGVTVAYLLGQEEPAPAADLGALLFRGGGENVSPEALPGLREFVDFLDR
                     YARLAQVTKFRIRALRQSPFVSAPGFESAEDARRKAEGVRESLRLGLGPVGDIDNVCE
                     MLGITVYRASLGEDLSRTVSGAFLNHAEIGLSILVNLAMTPGRRRFTVAHELAHALFH
                     SDEHRYVVSTAAKDPRERFADAFAAEFLMPHEGVRRVMEEQGFGPKIEDVSEVIHIQR
                     YFGVSFITALVRLRQARVINQTQLDEFKMIRPAVMARALGYEVTEDEFGPSHIRSRLD
                     RYPPRFRRLLRTAVRQGVLSVPSAAALTHAPIEEVAELVVMTP"
BASE COUNT          478 a          972 c         1000 g          515 t
ORIGIN      
        1 cggcgccgac gaccacgccc tggccgcgaa gctcaccatc ccgacgctcg tcgccgtcgc
       61 cacgaacgat ccgcactgcc ccctgggcga cgtgcaatcg ttgtatgacg cggtcgcggc
      121 acgcacgaag caactgagca tcctggacca gaacccgggc cggcatggct gggacctgct
      181 cctcgaggcc ggcgcgccgc taccgctggc caccatcgtc gccgactgga tcacccacgc
      241 cggcccgcgc cgaccctgac ggctccagat cagccggtac gggccggagg catcgggccg
      301 ttgtcgcgag cccactcttc gatgatctcc cgcatgagcg tggatacacc gatgccccgt
      361 gccgccgcgg cggtttcgag ctcggcccgg atatcgagat ctatgcgcag ggcggtgacc
      421 atcgtggcgg gtcgccgctc agggacgacg tcagcttcca cgaacatgtc gcccacgtcg
      481 ttgttcatca catgctcggc ggctgcctcg ggggcatacc agcgagcttg cgtcggctgg
      541 tggtcatgcc gtgtgcttcc tccacatcgc acgttcgttg ggtcctgcgt cacgggcacc
      601 gacaattggg ttcatcgtcg ggccgcgagc cacgtgagtg cgcgttcagc ggccggacgg
      661 tgactcggcg acacatcggc gagacgcgtt gcgatcaatc gcgccccgac gagcccagca
      721 tcgagtaacg cagtgacgaa gttctggtcc ttctcgcgga acgcgcagag tttggcgaca
      781 cagatgtctt ccttgtcgag acaccacccg atgtactgcg gtctacccga tggcgcggcg
      841 gtgttggcgt tctggacctt gaccaggcgg tcccgccagc cgcccggcag gacagccgtg
      901 tcctcgtcga cgccgtcaat gctgaagcca tgctgcccct cgaacggcga ccactcgccc
      961 gcgacgccct cgatcttgtc ggcaaggcga gccgcctcct cgttgctctc ggcgatcggg
     1021 aggatgtcga cctcgaccga catggttgcc tcgggcggta actggtcctc ggtgtaggtg
     1081 ccgagaatcg cctgcgagcc gaccacgatg acggcgggcc gctcgatgac ctggcaggcc
     1141 gtacggatgg cgtgctcgag ctggtcgcgt cgcatcagct cgcgttccgc aggacctggc
     1201 tgcgatcgtg ctgcggcagc agaccaccca tcggggagac ctcgcgcatc tggatcgagt
     1261 cggaatcgag ccctgtcatc acccgtcgca ggcccgagat gtcaccctcc tcgatgaggc
     1321 gttgccaccg gtcgaggttt cgtaggtgag gctgaccacg catactcgcc cggaggcgac
     1381 ggatgttgcg cagcatgggt tcgcgccagt ggtcgaagct ctcgggtgtg agatccatcg
     1441 atagccgacg gtgtagccgc caggagcgct cgggcgagcg accgagttgg tgcttgaccg
     1501 gtcgccggac gacctccagg acgtagccca tgcaggacag caggcggtcg agcatgtcgt
     1561 cgctcatcgc ggtgcgaccg gagaggaact ggctgatgct gggctgccgc acaccgctga
     1621 cccgggacag ctccgactgg gtcgtgccgg tctcccgcat cacctcacgc agaacctcgc
     1681 cacggctggg catagcaggc actatagcag atagtgctat gccgagccgc gcgacagtgt
     1741 agtcgacctc gttggcgact acgacccagg tgttgcgcgt ttgccggcgc tatgctggga
     1801 tcagtaacag gcgagacaac gggaggtgag cgttcgtgcc cctaggcgag caattggccg
     1861 ctgcgcgcgc tcgtagcggt tacgaccagg acgacgtggc caacgggctg ggcgtcagcc
     1921 gagccatggt gagctactgg gagtccggcc ggcggacgcc gaacgaccga cagatggtgg
     1981 ccctcgcaca gctgctcggg gtcacggtcg cctacctgct gggccaggag gagccggctc
     2041 cggccgcgga ccttggcgca ttgctgtttc ggggcggtgg cgagaatgtg tccccggaag
     2101 cgttgcctgg tcttcgcgag ttcgtcgact tccttgaccg ctatgcccga ctcgcacagg
     2161 tgacgaagtt tcggatccgt gcgctccggc agagcccgtt cgtgagcgca cccgggttcg
     2221 agtccgcgga agacgcgcgc cgcaaggcgg agggcgtgcg ggagagcctg cgactcgggc
     2281 tcgggccggt cggcgacatc gacaacgtgt gcgagatgct gggaatcacg gtgtaccgag
     2341 cgtcgctggg cgaggatctg tcgcggacgg tgtccggagc gttcctgaac cacgctgaga
     2401 tcgggctatc catcctggtg aacctcgcga tgacccctgg tcgacgcagg ttcaccgtgg
     2461 ctcacgaact ggcgcacgcc ctgttccaca gcgacgaaca tcgttacgtc gtgtcgacgg
     2521 ccgcgaagga ccctcgggag cgcttcgcgg atgccttcgc cgccgaattc ctcatgccgc
     2581 acgagggcgt ccggcgtgtc atggaggagc agggcttcgg cccgaagatc gaggacgtct
     2641 ccgaagtaat ccacatacag cgatacttcg gtgtctcgtt catcacggcc ctggtgcggt
     2701 tgcggcaggc gagggtgatc aatcaaacgc agctcgacga gttcaagatg atccggccag
     2761 cggtgatggc tcgggcgttg ggctacgagg tcaccgagga cgagtttggt ccgtcacaca
     2821 tacgatcgcg cctggaccgg tatccgccgc gattccgtcg cctgctacgg actgcggtgc
     2881 gtcagggcgt cctctccgtt ccctccgccg ccgcactgac gcacgctccg atcgaggagg
     2941 tggccgagct tgtcgtgatg acccc
//
LOCUS       DATBFZ010000401         1931 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_2250595, whole genome shotgun sequence.
ACCESSION   DATBFZ010000401 DATBFZ010000000
VERSION     DATBFZ010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 1931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1931
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2250595"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="VH419_12070"
     CDS             <1..181
                     /locus_tag="VH419_12070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY7044396.1"
                     /translation="EHKQASLEELGQLHEPVLTKDAIAGRIRRLLAMADKRAEELGVP
                     DTESSLTPEMLAEDD"
     gene            379..1404
                     /gene="gap"
                     /locus_tag="VH419_12075"
     CDS             379..1404
                     /gene="gap"
                     /locus_tag="VH419_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEY7044397.1"
                     /translation="MTVRVGINGFGRIGRNFFRAVQASDADVEVVGVNDLAANDILAH
                     FLRYDSILGRYPGEIESDESYLTVDGTKIHATAERDPAALNWGEIGADVVIESTGFFT
                     KADDARKHLEAGAKKVIISAPATDEDITVVLGINEDSYDASKHHVLSMASCTTNCLAP
                     MAKVLLDNWGIEKGFMTTVHAYTTEQQLLDQISLNRKGQINLRRARAGASNIIPTTTG
                     AAKAISLVLPELKGKLDGYALRVPVPTGSVTDLTVTLGGEVTKDEVNAAFKAAAESGP
                     LSPYLEYTEDEIVSADIVTWPASCTLDAKCTLANGNQVKVVGWYDNEWGYSNRLIDLS
                     ALVGSSL"
     gene            1413..>1931
                     /gene="pgk"
                     /locus_tag="VH419_12080"
     CDS             1413..>1931
                     /gene="pgk"
                     /locus_tag="VH419_12080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007077853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEY7044398.1"
                     /translation="MAGTDSSSEPLADRLGDVRGRRVLVRADLNVPLDAGRITDDGRV
                     RASLPTIEELSDAGARVVVISHLGRPKGEPDDRYSLRPVAARLGELLGREVAFAEDTV
                     GPSAQKTVDGLADGDVAVLENLRFNPGETSKDDAERGRFADQLVELVGPSGAFVSDGF
                     GVVHRKQASVYDV"
BASE COUNT          339 a          678 c          647 g          267 t
ORIGIN      
        1 ggagcacaag caggccagcc tcgaggagct cggtcagctc cacgagccgg tcctcaccaa
       61 ggacgccatc gccggccgca tccgccgact actcgcgatg gccgacaagc gcgccgagga
      121 gctcggcgtc cccgacacgg agtcctccct cacccccgag atgctcgccg aggacgactg
      181 acgttgggat acccggctcg ccgggtattg ctgcacttct caggggttgc aacccacgag
      241 aagcgacggc agtgcccccg cggtgtgact cgtggggcag gggtacgccg gcccggaagg
      301 cagcacctca cacaaggatc ggagaaacct ggcgataggc tcgagctacc gtagcccgcg
      361 acttgctagg agcatcccgt gaccgttcga gtaggcatca acggcttcgg ccggatcggc
      421 cgcaacttct tccgcgccgt ccaggcatcc gacgccgacg tcgaggtcgt cggtgtcaac
      481 gacctcgcgg ccaacgacat cctggcccac ttcctccgct acgactcgat cctggggcgc
      541 tacccgggcg agatcgagtc cgacgagagc tacctgaccg tcgacggcac caagatccac
      601 gcaaccgctg agcgcgaccc ggctgcgctc aactggggtg agatcggcgc cgacgtggtg
      661 atcgagtcga ccggcttctt caccaaggcc gacgacgcgc ggaagcacct cgaggccggc
      721 gccaagaagg tgatcatctc agcgccggcc accgacgagg acatcacggt cgtgctcggg
      781 atcaacgagg actcctacga cgcctcgaag caccacgtgc tgtccatggc cagctgcacg
      841 accaactgcc tggcgccgat ggccaaggtc ctgctggaca actggggcat cgagaagggc
      901 ttcatgacca cggtccacgc ctacacgacc gagcaacagc tgctcgacca gatctccctc
      961 aaccgcaagg gtcagatcaa cctgcgtcgc gcccgcgccg gcgccagcaa catcatcccg
     1021 acgaccaccg gagcggcgaa ggccatctcg ctggtcctgc ccgagctgaa gggcaagctc
     1081 gacggctacg cgctccgcgt cccggtgccc actggttccg tgaccgacct gaccgtgacc
     1141 ctcggcggtg aggtgaccaa ggacgaggtg aacgccgcgt tcaaggccgc ggccgagtcg
     1201 ggtcccctct cgccgtacct ggagtacacc gaggacgaga tcgtcagcgc cgacatcgtc
     1261 acgtggccgg cgtcgtgcac gctcgacgcg aagtgcaccc tggccaacgg caaccaggtc
     1321 aaggtcgtcg gttggtacga caacgagtgg ggctactcca accggctgat cgacctgtcg
     1381 gcgctcgtcg gctcctcgct ctgaggaccg gcgtggcggg gaccgactcc tcgagcgagc
     1441 cgctggcgga ccggctcggc gacgtccgtg gcaggcgggt cctggtccgg gccgacctca
     1501 acgtgccgct cgacgccggc aggatcaccg acgacggtcg ggtgcgcgcc agcctgccga
     1561 cgatcgagga gctctcggac gccggcgccc gggtcgtggt gatctcgcac ctgggacggc
     1621 cgaagggcga gccggacgac cggtactcgc tgcgccctgt ggcagcccgc ctgggcgagc
     1681 tgctgggacg cgaggtcgcc ttcgccgagg acaccgtggg accgtcggcg cagaagacgg
     1741 tcgacgggct cgcggacggt gacgtcgcgg tcctggagaa cctcaggttc aaccccggtg
     1801 agaccagcaa ggacgacgcc gagcggggcc ggttcgccga ccagctcgtc gagctggtcg
     1861 ggccgtcggg cgccttcgtg tccgacggct tcggggtcgt gcaccgcaag caggccagcg
     1921 tgtacgacgt a
//
LOCUS       DATBFZ010000402         8516 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_3559753, whole genome shotgun sequence.
ACCESSION   DATBFZ010000402 DATBFZ010000000
VERSION     DATBFZ010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 8516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8516
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3559753"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1161)
                     /locus_tag="VH419_12085"
     CDS             complement(<1..1161)
                     /locus_tag="VH419_12085"
                     /inference="COORDINATES: protein motif:HMM:NF019935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HEY7044399.1"
                     /translation="MPTTGVALVAVGGYGRAELSPCSDLDLVLLAADPFDERHLAALA
                     DALWYPLWDDQVPIDHAVRTESELRAAAAGDLRVALGTLDLRHVSGDPTLTMSTRSTV
                     LVDWRRGAKRRLPELAAATRERWSRLGDLAHATTPDLKEARGGLRDVSMLRALLASWL
                     VDVPAAELDRLARELLDVRDALHETTGRHTDRLVAEYLPDVAKRLGPRDPETVKSSLV
                     ETGRAIGHLAAVALRTVDWLTTPAKVGARRPVLDVLAEGVGTYRGEVVLTEKADPTRD
                     PLLALRAAEAAATNGLVLTDAAAARLGRELAPLPVRWPGEARALFGRLLASGPALVDV
                     WESLEQYGVVESWLPEWKRVRHLAPQAAVHRFTVDRHLVQTCVETAPYLPHLD"
     gene            complement(1291..1629)
                     /locus_tag="VH419_12090"
     CDS             complement(1291..1629)
                     /locus_tag="VH419_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018850023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEY7044400.1"
                     /translation="MKLVTAVVKPHKLEDVRAAVEAFGVTGMTVTEASGYGRQKGHTE
                     VYRGAEYDIALVPKVRLDIVVEDDETDDIVGVIVKSAQTGRIGDGKVWVTPVETVVRV
                     RTGDRDSDAI"
     gene            complement(1626..2996)
                     /locus_tag="VH419_12095"
     CDS             complement(1626..2996)
                     /locus_tag="VH419_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012922352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEY7044401.1"
                     /translation="MNVDTGDTAWVLMSSALVLLMTPGLAFFYGGMVRSKAVLNMLMM
                     SMLTIGIVTPLWVLYGFSLSFGTENASGLIGDFSAAGLSDMLDTTAVNGANYPIPILV
                     FAAFQLMFAIITPALISGAIADRAKFGAWALFVAAWVTLVYFPVAHWVFAFGDAAGNG
                     AGWLANWGVLDFAGGTAVHVNAGAAGLALAIVLGRRIGWRRDPMRPHSLPLVMLGAGL
                     LWFGWFGFNAGSALGASSAAAMAFMNTQVATATAMLGWLLVEKVRDGHATTLGAASGA
                     VTGLVAITPSCASVTPVGSIALGLIAGAVCAYAVGLKYRFGFDDSLDVVGVHLVGGAV
                     GSILIGLLAAPSSVMGATGVLYGGGADGWTLLGKQTVAVLSVGAFSFAVAFLIGKTID
                     LVMGFRISEEDEVTGIDQLAHAETAYDFHQAFGGVRTPALVTPGGSLAEPDDQQPVPD
                     KESVDA"
     gene            3413..4300
                     /locus_tag="VH419_12100"
     CDS             3413..4300
                     /locus_tag="VH419_12100"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HEY7044402.1"
                     /translation="MSTDLLSTRGLTAPLGVIRDRLAHELFARVAGPEGPARRDRIAT
                     APGPRWFDDDQPIREVHGDAAMFVGGLRALLFQSLHPVAMAAVTAHSGFRSDPWGRLH
                     RTSHFLAVTTFGAADDAEAMVAKVRAIHQRVRGRTADGVPYAASDPHLLKWVHLAEVD
                     SFLLAHTTYGRRPLEGASRDAYVAGTARVARALGVVDPPLTEAELCDQLAAYRAELRG
                     TKEARQAARFMLVNPPIPLALRPGYGVLAAAAVALMPAWSRWPLRLPYFPLTERVAVR
                     AAGNALTSTIRWALAPRSA"
     gene            complement(4384..5574)
                     /gene="ftsY"
                     /locus_tag="VH419_12105"
     CDS             complement(4384..5574)
                     /gene="ftsY"
                     /locus_tag="VH419_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEY7044403.1"
                     /translation="MALFLLALLIVVALVVAGLVTTRRSRGPVVGEEPSAPPREPAPA
                     AGDETRQAPEAAGEDAGLATEQVTLPEDADGILAGADTAVVELERPEPVAGRLQRLRA
                     RLARSQTTLGRGLLGLLSRDRLDDATWEEIEDTLLAADVGVAPTQELVDRLRTRVRVE
                     SASGQQAQDLLRDELVALVDPSFDRTLHADRRAGKPGVVMVVGVNGVGKTTTVGKIAR
                     VLVAEDKDVLLGAADTFRAAAADQLQTWGERVGVRTVRGDEGADPASVAFEATKAGVE
                     QEVDTVLIDTAGRLHTKTGLMDELGKVKRVVEKQASVDEVLLVIDATTGQNGLTQARI
                     FAEVVDVTGIVLTKLDGTAKGGIVIAVQRELGVPVKLVGLGEGADDLAPFEADTFVDA
                     LLDT"
     gene            complement(5602..5820)
                     /locus_tag="VH419_12110"
     CDS             complement(5602..5820)
                     /locus_tag="VH419_12110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044404.1"
                     /translation="MLVIWLGLVVILVLAGLVTLYAAYPHRGQDIPRAEWLSDAMRRS
                     NRTVNEKLARLDERVTQRGDDGTGGSTG"
     gene            complement(5941..>8516)
                     /locus_tag="VH419_12115"
     CDS             complement(5941..>8516)
                     /locus_tag="VH419_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020389382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY7044405.1"
                     /translation="DLEAQAERVRRQEAEIAAQVEEHRKALDAAVVAKRAAEEAAAEE
                     DKRIAGLQRAAADRREGLARLTGQVNTLKSRAAAAEDEIGRLGAARAEAITRAERAQH
                     DFTALETRVAGLDAGEQGLDAEHEGAVTLLHDLDDRLAKLRDEAQAAERERAALVARK
                     EALEIGLSRRDGAGALLAATDAVSGLLGSVAALLTVRQGYETALAAALGSAADAVAVA
                     DVGAAVGAIGHLKDADLGRAGLVLGGGSVDHEAWPVLRGGAVYAVDVVEAPGTLQPAL
                     RRLLFKTVVVDDLAAARDLVAGLPDVVAVTRDGDLVGAHYAAGGSSTQPSLIEVQAAV
                     DEAAEALAAATAGTERLRFELATLENDRQQAQHRVDVALAKLHESDAAMAAVAEELGQ
                     LGSLARTSKAEAERIGQAIVDAERARDADIAGLAELEHRLAAAASVPGHDEPDVAERE
                     RLAEAAQVARQSEMDARLALRTAEERARALHGRADALRKAAERERTALVRAKERRARR
                     LRDVRTAEAVGRAAHLVLDRLDASLGAAADRRTQVAEARSGRDDQLSKTRAAIRELEA
                     ELAQLTDTVHRDEMVRTERRMRMEQFEQRSLDEVGLELDSLVADYGPDQLVPPPPALP
                     DEVREVEPDPKPYVREEQEKRLRVAERALAQLGKINPLALEEFSALEERHKFLTEQLD
                     DLRRTRRDLLDIVRDVDERVEAVFSAAYADVAREFETVFARLFPGGVGTLVLTEPDDM
                     LATGIDVEARPPGKKVKRLSLLSGGERSLVAVAFLVALFKARPSPFYILDEVEAALDD
                     TNLGRLLEIYEELREDSQLVVITHQKRTMEVADALYGVSMRGDGVSAVISQRLREAES
                     A"
BASE COUNT         1233 a         3088 c         2895 g         1300 t
ORIGIN      
        1 gtcgaggtgg ggcagatacg gcgccgtctc aacacaggtc tggaccaggt gccggtccac
       61 tgtgaaccga tgcaccgctg cctgcggggc aaggtgccgc acacgtttcc actccggcag
      121 ccaggactcc acgacgccgt actgctcgag gctctcccag acgtcgacca gcgcgggtcc
      181 ggaggccagc aaccggccga acagcgcacg tgcctcgccc ggccaccgca ctggtaacgg
      241 ggcaagctcg cgccccagac gggcggccgc tgcgtcggtg agcaccaggc cgttcgtcgc
      301 agcggcctcg gccgcacgca gcgcgagcag cggatcccgg gtggggtcgg ccttctcggt
      361 cagcacgacc tcgccgcggt acgtaccgac accttcggcc agcacgtcga ggaccggacg
      421 acgggcaccg accttcgccg gtgtcgtcag ccagtcgacg gtccgcaacg ccaccgcagc
      481 caggtgcccg atcgctcgcc cggtctcgac cagcgacgac ttcaccgtct ccgggtcacg
      541 ggggccgaga cgcttcgcga cgtccggcag gtactccgcc accagcctgt ccgtgtgccg
      601 gcccgtcgtc tcgtgcagcg cgtcgcgcac gtcgagcagc tcccgggcga gccggtccag
      661 ctccgccgcc ggtacgtcga ccagccagct ggcgagcaat gcacgcaaca tgctcacgtc
      721 acgcaggcca cctctggcct ccttgaggtc aggcgtcgtg gcgtgcgcga ggtcgccgag
      781 gcggctccag cgttcccggg tggccgccgc cagttctggc agccggcgct tggctcctcg
      841 acgccagtcg accagcactg tgctgcgggt gctcatggtg agcgtcgggt cgccggatac
      901 gtgacgcagg tcgagcgtcc cgagcgccac ccgcaggtcg ccggccgcgg cagcgcgcag
      961 ctcgctctcg gtccggacgg cgtggtcgat cggcacctga tcgtcccaga gcggatacca
     1021 cagcgcgtcg gccagtgccg cgaggtggcg ctcgtcgaac gggtccgccg ccagcagcac
     1081 gaggtcgagg tcgctgcagg gagacagctc cgcgcgtccg tacccgccga cggcgacgag
     1141 ggccacgccc gtcgtgggca cctggtggtc ccgttggacc ctgtcccaca gcgagcgcag
     1201 cctctcgtct gccgaggcgg cccgggagcg ccgctgctcg gaccacgtcg ggggctggcg
     1261 tggtgtcctc acagcagcag ctctgccgtc tcagatcgcg tccgagtccc ggtcgcccgt
     1321 ccggacccgg acgactgtct ccaccggcgt cacccagacc ttgccgtcgc cgatgcggcc
     1381 cgtctgcgcg ctcttgacga tcacgccgac gatgtcgtcc gtctcgtcgt cttctaccac
     1441 gatgtccaga cgcaccttcg ggacgagcgc aatgtcgtac tccgcgcccc ggtagacctc
     1501 ggtgtgcccc ttctggcggc cgtagccact tgcctcggtg acggtcatcc cggtcacgcc
     1561 gaacgcttcc accgctgccc gcacatcctc gagcttgtgc ggcttgacca ccgctgtcac
     1621 gagcttcatg cgtccacact ctccttgtcc ggaacgggct gctgatcatc aggctcggcg
     1681 agcgacccgc cgggcgtgac cagggcgggt gtgcgtactc cgccgaacgc ctggtggaag
     1741 tcgtacgccg tctccgcgtg tgccagctgg tcgatgccgg tcacctcgtc ctcctcgctg
     1801 atgcggaaac ccatcacgag gtcgatcgtc ttgccgatga ggaaggccac cgcgaaggag
     1861 aacgcgccga ccgagaggac tgccacggtc tgcttgccca gcagggtcca gccgtcggcg
     1921 ccgccgccgt acaggactcc ggtggcaccc atgacgctgc tgggcgcggc gagcagcccg
     1981 atgaggatcg agccaacggc gccaccgacc aggtgcacgc ccacgacgtc cagcgagtcg
     2041 tcgaacccga accggtactt gaggcccact gcgtacgcgc acaccgcacc cgcgatgagc
     2101 ccgagcgcga tcgagccgac aggcgtgacg ctggcgcacg acggcgtgat ggcgacgagg
     2161 ccggtgacgg cgccggacgc tgcgccgagc gtggtcgcgt gcccgtcgcg gaccttctcg
     2221 acgagcagcc agccgagcat cgcagtggcg gtggcgacct gggtgttcat gaacgccatc
     2281 gcggcggcgc tgctcgctcc gagtgcagag ccggcgttga aaccgaacca cccgaaccag
     2341 agcagtccgg caccgagcat gaccaggggc aggctgtgcg gacgcatcgg gtcacgacgc
     2401 catccgatcc gcctgccgag cacgatggcg agtgcgagac ctgctgcacc ggcgttgacg
     2461 tgcaccgccg taccgccggc gaagtccagc acgccccagt tggccagcca tcccgcaccg
     2521 ttgcccgcgg cgtcaccgaa cgcgaacacc cagtgcgcaa ccgggaagta cacgagcgtg
     2581 acccaggccg ccacgaacag cgcccaggcg ccgaacttcg cccggtcggc gatggcgccg
     2641 gagatcagcg ccggcgtgat gatcgcgaac atgagttgga acgcggcgaa gacgagaatc
     2701 gggatcgggt agttcgcacc gttcaccgcc gtcgtgtcga gcatgtcgga gagcccggcc
     2761 gcgctgaagt cgccgatcag cccggaggcg ttctcggtcc cgaacgacag cgagaacccg
     2821 tagagcaccc acagcggtgt gacgatcccg atcgtcaaca tgctcatcat cagcatgttg
     2881 aggacggcct tggaacggac catgccgccg tagaagaacg cgagtccggg agtcatgaga
     2941 aggacgagcg cggagctcat caggacccac gcggtgtcgc cggtgtcgac gttcatccgg
     3001 atgctcctgt cggtcagcgg gagtgtcggt cgggacggcg ttgtctcttc gagtcctcac
     3061 tgtggcgtcc ggaggtttca gcaagtctcg tgtcgtgttg cgcgcacgtg acacctctcc
     3121 cacccgtgtt acatccacgt gaactcccca gtccccggcc gaggtggcag gggtgtcagg
     3181 tgggtcccgc tcgtcgcgag cggggtcgag tggatgagct gccaggcgga ggaggagatc
     3241 gatgcccagc atcgggcgac gactccaacg ccgcagatcg ccgcctcggg cacgcgcagc
     3301 agagcatgga tccacctgag acctccaatt ggttgcggac acgccggttc gtggaacgaa
     3361 cgtgccacct cggcggcggg cgcgacgatc gatcgcaagg tttactgggt gggtgagcac
     3421 ggacctgctg tccacgcgtg ggctgaccgc gccgctgggc gtcatccggg atcggttggc
     3481 gcacgagctg ttcgcgcgag tcgccggccc ggaaggtccg gcccgtcgtg accgcatcgc
     3541 aaccgccccg gggccgcgct ggttcgacga cgatcagccg atccgggaag tgcacggtga
     3601 tgcggcgatg ttcgtcggtg gcctgcgtgc attgttgttc cagtcgttgc acccggtcgc
     3661 gatggctgcc gtgacggcgc actcaggctt ccgcagcgat ccttggggcc ggctgcaccg
     3721 gaccagtcat ttcctcgccg tgaccacgtt cggggcggcc gacgatgccg aggcgatggt
     3781 cgcgaaggtg cgcgctatcc accagcgcgt ccgaggacgt accgctgacg gcgtcccgta
     3841 cgccgcgtcc gacccgcatc tgctcaagtg ggttcatctc gccgaggtgg acagcttcct
     3901 gttggcgcac acgacgtacg ggcgtcgccc gctcgaggga gccagccgcg acgcgtacgt
     3961 cgccggcacg gcccgcgtcg ccagagcgct cggagtggtc gacccgccac tgacagaggc
     4021 ggagctgtgc gaccagctgg cggcgtaccg cgctgagctc cgtggcacca aggaggcccg
     4081 gcaagcggcg cgtttcatgc tcgtgaaccc gccgataccg ctggcgttgc gaccggggta
     4141 cggcgtgctc gccgccgcgg ccgtcgccct catgccggcg tggtcacggt ggccgcttcg
     4201 cctgccgtac ttcccgctga ccgagcgagt cgcagtccga gcggcaggaa acgcgctcac
     4261 cagtacgatc cgctgggcac tcgctccccg gtctgcgtga acccctcacc gaggtggcaa
     4321 tcggcgtccg acacgccgtc aaaacccaac caaacttgcc acctcggcgg gagtcggcgg
     4381 aggtcaggtg tcgaggaggg cgtcgacgaa ggtatcggcc tcgaacggtg ccaggtcgtc
     4441 ggcgccttca cccagaccga cgagcttcac gggtacgccg agctcgcgct gtaccgcgat
     4501 gacgatgccg cccttcgccg taccgtcgag cttcgtcaac acgatgcccg tgacgtcgac
     4561 gacctcggcg aagatccggg cctgggtgag gccgttctgt cccgtggtcg cgtcgatgac
     4621 gagcagcacc tcgtcgacgc tcgcctgctt ctccacgacc ctcttgacct tgccgagctc
     4681 gtccatcagc ccggtcttgg tgtgcagccg gccggccgtg tcgatgagca cggtgtcgac
     4741 ctcctgctcg actccggcct tcgtggcctc gaaggcaacg ctggccggat cggcgccttc
     4801 gtcgccccga accgtacgga caccgacgcg ttcgccccac gtctggagct ggtccgcggc
     4861 cgccgcgcgg aaggtgtcag ccgcccccag cagcacgtcc ttgtcctcgg cgacgagtac
     4921 gcgggcgatc ttgccgaccg tggtcgtctt ccccacaccg ttgacaccca cgaccatgac
     4981 gacaccgggt ttcccggccc tccggtccgc gtgcagggtg cggtcgaacg acgggtccac
     5041 cagcgcgacc agctcgtcgc gtagcaggtc ttgggcctgc tgcccggacg ccgactcgac
     5101 ccgcacacgc gtgcgcagcc ggtcgaccag ctcctgggtc ggcgccactc cgacgtcggc
     5161 cgcgagcagc gtgtcctcga tctcctccca cgtggcgtcg tcgagacggt cccgcgagag
     5221 caggcccaga agccctcgac ccaacgtcgt ctgcgaccga gcgaggcggg cacgcagtcg
     5281 ctgcagccgt cccgcgaccg gctcggggcg ctccagctcg acgacggcgg tgtccgcgcc
     5341 ggcgagtatg ccgtccgcgt cttcaggaag ggtgacctgc tccgtggcga ggccggcgtc
     5401 ttcgcctgca gcctcgggcg cctgccgggt ctcgtcgcca gccgccggag cgggttcccg
     5461 tggcggtgcc gacggctctt caccgacaac cgggccgcgg ctgcgacggg tcgtgacgag
     5521 ccccgcgacg acgagcgcga cgacgatgag tagggctagg aggaagagcg cgatggtcac
     5581 gacgcaccat cctcgcagac gtcagccagt agacccgccg gtgccgtcgt cacctcgttg
     5641 cgtgacccgt tcgtcgagcc gggcgagctt ctcgttgaca gtccggttgg atcgacgcat
     5701 cgcgtccgac aaccactctg cgcgcggtat gtcctggcca cgatgcggat aggcggcgta
     5761 cagcgtgacc agtcctgcca ggaccaggat cacgacgagt ccgagccaga tgacgagcat
     5821 ggtgcgggcc tcctgcctta cgacggtgtg gtcgtgctct cccctgcaac cgttccatcg
     5881 tgccaggtgc tgaccaccga ccccaatccg gcgctctcgc ggagcgtcgc ggacgccggc
     5941 tcaggcggac tccgcctcgc gcaaccgctg gctgatcacc gccgagacgc cgtcgccgcg
     6001 catcgacacg ccgtacagcg cgtcggcgac ctccatggtg cgcttctgat gggtgatgac
     6061 gaccagctga gagtcctccc gcagctcctc gtagatctcg agcagcctgc cgaggttcgt
     6121 gtcgtcgagg gctgcctcga cctcgtcgag gatgtagaag ggcgacggcc tggccttgaa
     6181 cagcgcgacc aggaacgcaa cggcgaccaa cgagcgttcg ccgccggaca gcaacgacaa
     6241 gcgcttgacc ttcttgccgg gaggacgtgc ctccacgtcg atgccggtcg ccagcatgtc
     6301 gtcgggctcg gtgaggacga gcgtgcccac gccgccgggg aagagccggg cgaagaccgt
     6361 ctcgaactcg cgggcgacgt cagcgtacgc cgccgagaac accgcctcga cccgctcgtc
     6421 gacgtcgcgc acgatgtcga gcaggtcgcg acgggtcctg cgcaggtcat cgagctgttc
     6481 ggtgaggaac ttgtgccgtt cctccagtgc ggagaactcc tcgagcgcca acgggttgat
     6541 cttgcccagc tgcgccagcg cgcgttcggc cacgcgcagc cgcttctcct gctcttcgcg
     6601 gacgtagggc ttcgggtccg gctcgacctc acgtacctcg tcgggcaacg ccggcggagg
     6661 cgggacgagc tggtcgggtc cgtagtcggc gacaaggctg tccagctcga gaccgacctc
     6721 gtcgagcgag cgctgctcga actgttccat ccgcatccgg cgctcggtgc gcaccatctc
     6781 gtcgcggtgc accgtgtcgg tgagctgcgc cagctccgcc tcgagctcgc gaatggcggc
     6841 ccgggttttc gacagttggt cgtcccggcc ggatcgggcc tccgccacct gggtacgacg
     6901 gtcggctgct gcgccaagcg aggcgtcgag tcgatccagc accaggtggg cggctcggcc
     6961 gaccgcctcc gccgtacgca cgtcgcgaag ccgtcgagcg cgtcgctcct tcgctcggac
     7021 cagggccgtc cgctcccgct ccgcagcctt ccgcaacgca tcagcacgcc cgtgcagggc
     7081 gcgggcgcgt tcttccgcgg ttcgcagcgc cagccgcgcg tccatctcgg actgccgtgc
     7141 cacctgcgca gcctcggcca gccgctcgcg ttcggcgacg tccggctcat cgtggccggg
     7201 caccgaggcc gccgccgcca gtcggtgttc gagctcggca agtccggcga tgtcggcgtc
     7261 cctggcccgc tcggcgtcga cgatggcctg gccgatccgc tcggcctcgg ctttcgaggt
     7321 gcgcgccagc gagccgagct ggccgagctc ctccgccacc gccgccatcg cggcgtccga
     7381 ctcgtgcagc ttggccagcg cgacgtcgac acggtgctgc gcctgctgtc ggtcgttctc
     7441 gagcgtcgca agctcgaagc gcagacgttc cgtaccggcg gtcgcggccg cgagtgcctc
     7501 ggcggcctcg tcgacagccg cctgcacctc gatgaggctc ggttgggtcg atgacccacc
     7561 ggccgcgtag tgtgccccga ccaggtcgcc gtccctggtc acggcgacca cgtccgggag
     7621 ccctgccacc agatcacgcg cagcggcgag atcgtcgacg accacggtct tgaacagcag
     7681 tcggcgcagc gcaggttgca gtgtgccggg cgcttcgacc acgtcgacgg cgtacaccgc
     7741 tccgccgcga aggaccggcc aggcctcgtg gtcgaccgaa ccgccgccca gcacgagtcc
     7801 ggcccggccg aggtcggcgt ccttgagatg accgatcgcg ccgacggcag cccccacgtc
     7861 ggccaccgca acggcgtccg ctgcggagcc gagtgcggcg gccagtgcgg tctcgtagcc
     7921 ctgacggacg gtcagcagcg cggcgaccga gccgagcagt ccggagaccg catcggtcgc
     7981 agccagcaag gcgcccgcac catccctgcg ggacaggccg atctcgagcg cctctttgcg
     8041 cgcgacgagc gcggcccgtt cccgctcggc ggcctgcgcc tcgtcacgta gcttggcgag
     8101 ccgatcgtcg aggtcgtgca ggagtgtcac ggccccctcg tgctcggcat ccagaccctg
     8161 ttcgccggcg tcgagcccag cgacccgcgt ctcgagagcg gtgaagtcgt gctgtgcgcg
     8221 ctctgctcgg gtgatcgcct ccgcgcgcgc agcccccagt cgcccgatct cgtcctcggc
     8281 ggcggcagcg cggctcttga gcgtgttgac ctggccggtg agccgggcca gtccctcgcg
     8341 gcggtcggcg gcggcgcgct ggagtccggc gatccgcttg tcctcctcgg ccgcggcctc
     8401 ctcggctgcg cgcttggcga ccacggcggc atcgagggcc ttgcggtgct cctcgacctg
     8461 cgccgcgatc tcggcctcct gccggcgcac gcgctcggcc tgggcctcga ggtcgt
//
LOCUS       DATBFZ010000403         7853 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_2571251, whole genome shotgun sequence.
ACCESSION   DATBFZ010000403 DATBFZ010000000
VERSION     DATBFZ010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 7853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7853
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2571251"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="VH419_12120"
     CDS             <1..291
                     /locus_tag="VH419_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020384933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HEY7044406.1"
                     /translation="QVERRINEEIERAHEIVVDFLPRNVADADPALIRTAANLIPAVI
                     DPLRIIDIVGLDRQADGGTHVLSTAEVGRVKVTGTESKGKDNKRIRLEVVDA"
     gene            288..842
                     /locus_tag="VH419_12125"
     CDS             288..842
                     /locus_tag="VH419_12125"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEY7044407.1"
                     /translation="MTHHLVVTQNMTLDGRIEFLDTWFDPTMKYGDNTDVLEEMDRQG
                     RECAGMLLGRKTFEEFRGYWPQHEDEPAGASLARLNKYVVSRTLGDPAWQRSTVLSGD
                     PLEEVRSLKNGGDGDLVVTGSITLTHALFDAGLVDEVRLFVYPAIQGRGRGLVDESIS
                     LYGLTLLESRSFRGGVTLLRYAVG"
     gene            1005..2276
                     /locus_tag="VH419_12130"
     CDS             1005..2276
                     /locus_tag="VH419_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044408.1"
                     /translation="MATTPQHRQPTRDHLIGLLLGAEEDWPQAFEQILRFVGPLDVDG
                     TEHRIASERLTIEPFDLTDPVRTGLVIDRLAHWYFHPREWLKKAALVNGTYLLNSPFT
                     FQSMEKHSAYCAMIRLGLKVPPTILVPYKNPLDNHRWAYTSEKYNKPFDLDVVATEIG
                     YPLYMKPFDGGGWRGVSRVNDQHDLHTAYDDSGEMLMHLQATVDYEHFARALSIGPET
                     MVMDFRPEQPMHQRYAVSHGFLSESAGKQAVAISRIVNAFFHWEFNSAEMLVAGDEVY
                     PIDYANACPDVAVTSLHYYFPWAISALVKWSTYCLVTGRRPNVDLEMTTYFEIADSDR
                     TYDEKLDAYLALADQHFETERYHAWCAEHLGHLDEAVAEWVSGPDFDRLLVETVQATY
                     PPHEHEQFLAHFRGLLGMWLSDQRSAATSPV"
     gene            complement(2313..3236)
                     /locus_tag="VH419_12135"
     CDS             complement(2313..3236)
                     /locus_tag="VH419_12135"
                     /inference="COORDINATES: protein motif:HMM:NF039362.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6159 family protein"
                     /protein_id="HEY7044409.1"
                     /translation="MATYDATWRPPEPPSSGSSVSALLVARVYRILLEDTSMVGVIAV
                     GALVSSLAFAGIAVPAWLLGGADLFSNPFSVRTILLYLVAGWAAAFVSVLFSGAVVAA
                     GIARLDGHPITVTAALGVAMRRWRQLAAWALVSTVVGFLAEQLNRFGILGNVLRVLAD
                     LAWALATLLVLPIIIVEGRMPTAAITESTSMVKHQLMLTVRTKVRFYLPLVVVMLLAA
                     LLTGAGLLVFIQYHHETPEWGIGGLLLAVVGFVLFVGAACVESAADALLNLVLYRHAL
                     GLPVPGVDARDLPRLPGTGEPSPPGWTTGMP"
     gene            complement(3401..4003)
                     /locus_tag="VH419_12140"
     CDS             complement(3401..4003)
                     /locus_tag="VH419_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE/ArgO family amino acid transporter"
                     /protein_id="HEY7044410.1"
                     /translation="MLDALVAGLLTGLSLIVAIGAQNAFVLRQGLLRQHVGPVVAICS
                     VSDLVLITAGVAGIGAIVQHAPTALDVVRWLGVAFLTAYGVRSLWRARHAGSLQASRE
                     SEPRLRGAVVQAVALTWLNPHVYLDTVLLLGSIAAHHGATGRWWFAVGAGLGSILWFT
                     GLGYGARLAAPLLARPRAWQVLDVLIGLTMLTIAVQLARG"
     gene            complement(4030..4215)
                     /locus_tag="VH419_12145"
     CDS             complement(4030..4215)
                     /locus_tag="VH419_12145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044411.1"
                     /translation="MGIGVSAVLLIVGLVLLTHAVDLPASVTDHVAANTVGWICLIVA
                     IVGFAIALLAGRGRQPR"
     gene            4380..7610
                     /locus_tag="VH419_12150"
     CDS             4380..7610
                     /locus_tag="VH419_12150"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEY7044412.1"
                     /translation="MRSSTRARSLCTATATALAAALIAAPATPAGAAPPAASRTPDPG
                     TQLTSSRPEVVTLLTGDRVILRHDTVGRTTAMLTPTSPHYGHRIEFLDTGSHSWVVPE
                     LAPSVRRRLDTSVFDVAALGRDGRVPLTVTFAKGATPRALPGLQVRTGSARRGSGGRT
                     TATASYDAHRPLPASFASSLRGVSRISVAGAAPQADDPAYQLHTLTINGTTAHGKPIP
                     GADVFVFNADDARLFGSFGGIVDGQWKVSVPSGNYIVLASDFRHAVVGQVSVTDDAVA
                     DVSMGDATVKPSMTAMKGLSALDPSLDVLGTDDAGYGGFDFGWSGFFPKVSPLTNVVA
                     GHVHTEVANLWSAKGYQPVTFHGGDVQVHPIRRVVSAKSVVKGIPHRLSFRFHRNDFA
                     DVAIKHYATGPTQKAFDSWVAISPVDQFLFVEGFPSVRPGVIHASFLGSKKLFWDSTT
                     SVSQSIRGFTELDQVATYHRHRHAQVPFFRGPVTPVADRGGNFEHASRFCRLCVTRGQ
                     LHGALAMFASAGTDQPGFDDHGRWELFRGHQQVDHGAGAIQPLVKGVVAGQRMRLEAT
                     LSPPDKKYVLSRHVSDTFRFQVPNDREMVPLLRAAYVPPTNLHGVGKAGRVSYPITFD
                     NLGPAAARVTHAAVRWSLDGRHWHRATLKRTDKNTFRVSYRNPVAGRAHRYLSLRVTG
                     RDQAGRSISETVEHAYVLPQGHARPSTHHATRRTTFRPGKLCRTSGEHAYSCYVKLSA
                     ATRSLGKASPDPAGWGAPALRSAYGITGDSQAPDTVAVIVAYDYPHAEADLNHYRRQY
                     GLPPCTSASGCFTKLNQKGQTDHYPAPDQGWGVEASLDLQMISATCPTCHIVLVEANQ
                     PTDKPFFRAEQTAVDAGATVTNHSFGRIELTGAETDASNYDHDGVTAVASTGDFGYQP
                     ASFPASSTDVVAVGGTVLARSTDERGFTEHAWRFGGSGCSAYFAKPAAQDDTACRGRT
                     MADVSAVAQGLAIYNTSLPRRFRGWLKVDGTSASSPLVAGMIASQGSGGIRPTALYAN
                     SDQFNDITGGSNGFCQGSYLCTGVPGYDGPTGLGSPRSPEAFVPPV"
BASE COUNT         1291 a         2850 c         2642 g         1070 t
ORIGIN      
        1 caggtcgagc ggcggatcaa cgaggagatc gagcgcgcgc acgagatcgt cgtcgacttc
       61 ctgccgcgca acgtcgcgga cgccgacccg gcactgatcc gcaccgcggc gaacctgatc
      121 ccggccgtga tcgacccgct ccggatcatc gacatcgtcg ggctcgaccg gcaggccgac
      181 ggcggcacgc atgtgctgtc gacggccgag gtcgggcggg tgaaggtcac cggcaccgag
      241 agcaagggca aggacaacaa acgcatccgg ttagaggtcg tcgacgcatg acacatcacc
      301 tcgtggtcac ccagaacatg accctggacg ggcgcatcga gttcctcgac acgtggttcg
      361 acccgacgat gaagtacggc gacaacaccg acgtcctgga agagatggat cggcagggcc
      421 gggagtgcgc ggggatgctg ctcggacgca agaccttcga ggagttccgt ggctactggc
      481 cccagcacga ggacgagccc gccggtgcct ccctcgcccg gctcaacaag tacgtcgtct
      541 cccgcaccct cggcgatccc gcgtggcagc gctccaccgt gctctcgggc gacccgctcg
      601 aggaggtgcg gtcgctgaag aacggcggcg acggcgacct ggtcgtcacc ggctcgatca
      661 ccctcaccca cgcgctgttc gacgccggcc tggtcgacga ggtccggctg ttcgtctacc
      721 ccgcgatcca gggccgcggt cgcgggctgg tcgacgagag catctcgctc tacggcctca
      781 ccctgctcga gtcgcggtcg ttccgcggcg gcgtcacgct gctgcggtac gccgtcggct
      841 gaacggcggt ttcgagacgg tcgcagagcg acctcctcaa ccaccgggga gttccccttc
      901 tccaaccacc cggggtgtcg tccggtggtt gaggaaggcg ccctggcgcc tgtctcgaaa
      961 ccatccctcc cacgggaccg tgccacgtgg ctagggtcgc gagcatggcc acgacacccc
     1021 agcaccgaca gccgacacgg gaccatctga tcggactcct gctcggggcg gaggaggact
     1081 ggccgcaggc cttcgagcag atcctgcgct tcgtcggccc gctggacgtc gacgggaccg
     1141 agcaccggat cgccagcgag cggctgacga tcgagccgtt cgacctcacc gacccggtcc
     1201 gcaccggcct ggtcatcgac cggctcgcgc actggtactt ccacccgcgc gagtggctga
     1261 agaaggcggc gctggtgaac ggcacctacc tgctcaacag cccgttcacg ttccagtcca
     1321 tggagaagca ctcggcgtac tgcgcgatga tccggctcgg gctcaaggtg ccgccgacca
     1381 tcctggtgcc gtacaagaac cccctcgaca accaccgttg ggcctacacc agcgagaagt
     1441 acaacaagcc gttcgatctc gacgtggtcg ccaccgagat cggctacccg ctctacatga
     1501 agccgttcga cgggggcggg tggcgcggcg tcagccgggt gaacgaccag cacgacctgc
     1561 acacggcgta cgacgactcc ggcgagatgc tcatgcacct ccaggccacc gtcgactacg
     1621 agcacttcgc ccgcgcgctc tcgatcgggc cggagacgat ggtgatggac ttccggcccg
     1681 agcagccgat gcaccagcgg tacgccgtca gccacggctt cctcagcgag tccgcgggca
     1741 agcaggcggt cgcgatcagc cgcatcgtca acgcgttctt ccactgggag ttcaactccg
     1801 ccgagatgct ggtggccggc gacgaggtct accccatcga ctacgccaac gcgtgcccgg
     1861 acgtcgcggt gaccagcctc cactactact tcccgtgggc gatctccgcc ctggtgaagt
     1921 ggtcgacgta ctgcctggta accggccgca ggccgaacgt cgacctcgag atgacgactt
     1981 acttcgagat cgcggactcc gaccggacct acgacgagaa gctcgacgcc tacctcgccc
     2041 tcgccgacca gcacttcgag accgagcggt accacgcctg gtgcgcggag cacctcggcc
     2101 acctcgacga ggccgtcgcc gagtgggtct ccgggccgga cttcgaccgg ctcctggtcg
     2161 agaccgtgca ggcgacgtac ccgccgcacg agcacgagca gttcctcgcc cacttccgcg
     2221 ggctcctcgg gatgtggctc agcgaccaac gcagcgctgc gacgagcccg gtctgacgcg
     2281 ctcagcctga gtgcgccgac gacggcgcgg acctacggca tgccggtggt ccaaccgggt
     2341 ggggacggct cgccggtccc cggaaggcgc gggaggtccc gggcgtcgac gccgggcacc
     2401 ggaagcccca gggcgtggcg gtacagcacg aggttgagca gcgcgtccgc cgcactctcc
     2461 acgcacgccg ccccgacgaa caggacgaac ccgacgacgg cgaggagcag gccgccgatg
     2521 ccccactcgg gggtctcgtg gtggtactgg atgaacacca gcagtccggc gccggtgagc
     2581 agcgcggcaa ggagcatcac caccaccaac gggaggtaga acctgacctt ggtgcgcacc
     2641 gtgagcatca gctggtgctt gaccatcgac gtggactcgg tgatcgccgc cgtgggcatg
     2701 cggccctcga cgatgatgat cgggagcacg agcagcgtcg ccagcgccca ggccaggtcg
     2761 gcgagcacgc ggagcacgtt cccgaggatc ccgaagcggt tgagctgctc ggcgaggaag
     2821 ccgaccacgg tgctcaccag tgcccaggct gcgagctgcc gccatcgacg catggccacg
     2881 ccgagggcgg ccgtcactgt gatcgggtgc ccgtcgagcc gggcgatgcc cgccgcgacg
     2941 acggcgccgc tgaagagcac ggagacgaag gctgcggccc agccggcgac caggtacagc
     3001 aggatcgtgc ggacactgaa cgggttggag aagagatcag cgccgccgag aagccacgcc
     3061 gggaccgcga tgcccgcgaa cgccagcgac gacaccagag caccgacggc gatgacgccg
     3121 accatgctgg tgtcctcgag caggatccgg tagacccgcg cgacgagcag ggcgctcacg
     3181 ctcgagccgc tcgacggtgg ctcaggtggc cgccaggtcg cgtcgtacgt cgccatctct
     3241 caccctcccg agtggactcc ggcggacagt ctgcgggatg actcgcccac gggtgggccg
     3301 ttcggcgaaa gagcggtagt ccccgacgtt tcggtccgcg acacgccggg gaagggaacc
     3361 gaaacgtcgg ggactatccc tcgaaaccgg gtgaccccac tcacccgcga gcgagctgga
     3421 cggcgatcgt cagcatggtc agcccgatca ggacgtcgag cacctgccag gcgcggggtc
     3481 gcgcgaggag tggtgccgcg agccgcgcgc cgtaccccag gccggtgaac cacaggatgc
     3541 tcccgagccc ggcgccgacg gcgaaccacc acctgccggt cgctccgtgg tgtgccgcga
     3601 tcgagccgag caggagcacg gtgtcgaggt agacgtgggg gttcagccag gtgagcgcaa
     3661 ccgcctgcac gaccgcgccg cgcagccgcg gctcgctctc ccgggacgcc tgcagcgacc
     3721 ccgcatgccg ggcccgccac agcgagcgca cgccgtacgc cgtcaggaag gcgacgccga
     3781 gccaccggac gacgtccaac gcggtcggag cgtgctggac gatcgccccg atgccggcga
     3841 cgccggctgt gatcagcacc aggtcgctga ccgagcagat ggccacgacc ggcccgacgt
     3901 gctggcgcag cagaccctgg cgcagcacga acgcgttctg ggcaccgatc gccacgatca
     3961 gcgagagccc ggtcagcaga ccggcgacca gggcgtcgag caccccacga ggctagtgag
     4021 gcctgccgat cagcgtggct gacgtccgcg gccggcgagc aacgcgatcg cgaagccgac
     4081 gatcgcgacg atcaagcaga tccacccgac ggtgttcgcc gcgacgtggt cggtcacgct
     4141 cgcgggcagg tccaccgcgt gcgtgagcag gacgaggccg acgatcagca gcaccgccga
     4201 gacaccgatg cccatgacgg ctcctttcgt cgtcaacccc cgtaccccgc ccggaccacg
     4261 ccacgcatcc gaaggtttcc ggccgggtca agactcgagc cgagcgtggc gcccacgcct
     4321 ttcgtcggcg tggacgtgtt gaatcccctt caggacttca cctcggggaa aggcttcaca
     4381 tgagatccag cacacgcgcg cgctcgctct gcaccgccac cgccacggct ctggcagcag
     4441 ccctgatcgc cgctccggcc acaccggcag gcgcggctcc gcccgccgcc tcccgcacgc
     4501 cggaccccgg cacgcagctc acgagctcgc gcccggaggt cgtgacgctg ctgaccggtg
     4561 accgtgtcat cctccgccac gacaccgtgg gacggacgac cgcgatgctg acgccgacgt
     4621 cgccgcacta cggacaccgc atcgagtttc tcgacaccgg gagccacagc tgggtggtgc
     4681 cggagctcgc accctcggta cgccggcggc tcgacacctc cgtcttcgac gtggctgcgc
     4741 tgggccgtga cggccgagtg ccgctcaccg tcactttcgc gaagggcgcg acaccgcgag
     4801 ccctgccggg cctccaggtc cgcacgggct ccgcgcggcg cggctccggt ggacgcacga
     4861 ccgccacggc gtcgtacgac gcgcatcggc cgctgcccgc ctcgttcgcc tcgtcgctgc
     4921 gcggcgtctc ccgcatctcg gtggcgggtg ccgccccgca ggccgacgac ccggcgtatc
     4981 agctccacac gctgacgatc aacgggacca ccgcgcacgg caagccgatc ccgggcgccg
     5041 acgtgttcgt gttcaacgcc gacgacgcgc gtctcttcgg ctccttcggc ggcatcgtcg
     5101 acgggcagtg gaaggtctcg gtcccgtccg ggaactacat cgtgctcgcc agcgacttcc
     5161 ggcacgcggt ggtcggccag gtgtcggtga ccgacgacgc ggtcgccgac gtctcgatgg
     5221 gcgacgcgac cgtgaagccg tcgatgacgg cgatgaaggg gctgtcggcg ctggatccct
     5281 cgctcgacgt gctgggcacc gacgacgccg gctacggcgg cttcgacttc ggctggtccg
     5341 ggttcttccc gaaggtcagc cccctgacga acgtcgtggc gggccacgtg cacaccgagg
     5401 tcgcgaacct ctggagcgcg aaggggtacc agccggtcac cttccacgga ggcgacgtac
     5461 aggtccatcc gatcaggcga gtggtgagtg ccaagtcggt cgtgaagggg atcccgcacc
     5521 gcctctcgtt ccgcttccac cgcaacgact tcgccgacgt cgcgatcaag cactacgcga
     5581 ccgggccgac gcagaaggcg ttcgacagct gggtggccat cagtcccgtc gaccagttcc
     5641 tgttcgtcga agggttcccg agcgtccggc ccggcgtcat ccacgccagc ttcctcggca
     5701 gcaagaagct cttctgggac agcaccacct cggtgtccca gtcgatccgc ggcttcaccg
     5761 agctcgacca ggtcgcgacg taccatcgtc accgccacgc gcaggtgccg ttcttccgcg
     5821 gaccggtcac accggtggcg gaccgcggtg gcaacttcga gcacgcgagc cgcttctgcc
     5881 ggctgtgcgt cacccggggt cagctgcacg gcgccctggc gatgttcgcc agcgccggca
     5941 ccgaccagcc gggcttcgac gaccacggcc ggtgggagct gttccggggt caccaacagg
     6001 tggaccacgg cgccggggcc atccagccac tcgtgaaggg cgtcgtggcc ggacagcgga
     6061 tgcggctcga ggcgacgttg tccccgccgg acaagaagta cgtgctgtcg cgccacgtgt
     6121 cggacacgtt ccggttccag gtcccgaacg atcgtgagat ggtcccgctg ctgcgcgcgg
     6181 cgtacgtccc gccgacgaac ctgcacggtg tcggcaaggc cgggcgcgtg tcgtacccga
     6241 tcacgttcga caacctgggc ccggctgccg cccgcgtcac ccacgcggcc gtgcgctggt
     6301 cgctcgacgg ccggcactgg caccgcgcca ccctgaagcg gacggacaag aacacgttcc
     6361 gcgtcagcta ccgcaacccc gtcgcaggaa gggctcaccg ctacctgagc ctgcgggtga
     6421 ccggtcgcga ccaggccggg cggtcgatct cggagaccgt cgagcacgcc tacgtgctgc
     6481 cgcagggcca cgcacgcccg tcgactcacc acgcgacccg gcgtacgacg ttccgcccgg
     6541 gcaagctctg tcgtacgtcg ggcgagcacg cctactcctg ctacgtgaag ctcagcgcgg
     6601 ccacccgatc gctcggcaag gcatcgccgg acccggccgg atggggtgcc ccggcgctgc
     6661 gcagcgcgta cggcatcacc ggcgactcgc aggcccccga caccgtggcc gtgatcgtcg
     6721 cctacgacta cccgcacgcc gaggcggacc tgaaccacta ccgccggcag tacggcctgc
     6781 cgccgtgcac gagtgcgagc ggctgcttca ccaagctcaa ccagaagggt cagacggacc
     6841 actacccggc gccggaccag ggttggggcg tcgaggcctc attggacctg cagatgatct
     6901 cggcgacgtg cccgacgtgc cacatcgtcc tggtcgaggc gaaccagccg accgacaagc
     6961 ccttcttcag ggcggagcag acggccgtcg acgcaggtgc gaccgtcacc aaccactcct
     7021 tcgggcggat cgagctgacc ggcgccgaga ccgacgcgtc gaactacgac cacgacggcg
     7081 tcacggccgt ggcgtcgacc ggtgacttcg ggtaccagcc ggccagcttc ccggccagct
     7141 ccaccgacgt cgtcgcggtg ggcggcaccg tgctcgcgag gagcaccgac gagcgcggct
     7201 tcaccgagca cgcgtggagg ttcggcgggt cgggctgctc ggcgtacttc gccaagccgg
     7261 cggctcagga cgacaccgcc tgccgcggcc ggacgatggc ggacgtctcg gcggtcgccc
     7321 agggtctggc gatctacaac acctcgctgc cgcgccgctt ccgcggctgg ctcaaggtgg
     7381 acggcaccag cgcctcgtcg ccactggtcg ccggcatgat cgcctcccag ggcagtggcg
     7441 gcatccggcc gacggcgctg tacgcgaact ccgaccagtt caacgacatc accggtggct
     7501 cgaacggctt ctgtcagggc agctacctgt gcaccggggt cccggggtac gacggtccca
     7561 ccggcctcgg gtcaccacgg tcaccagagg cgttcgtgcc gcccgtctga cgccgggagg
     7621 acgccgggag gacgccgagt cggcgcaagg tgacatgaaa tgagcgccga gtcggcgcac
     7681 agtgacatga aatgagcgcc gagtcgtcat atgacgactc ggcgctctcg gcgctctcgg
     7741 cgctctcggc gctctcggtg ctctcggcgc cgtcgacgcg gcgggcgtcc tcgacgctgc
     7801 cgacgctccc gacgcggcgg gcgtccactt tccgccagat caggcgtacc ggc
//
LOCUS       DATBFZ010000404         6724 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_1922692, whole genome shotgun sequence.
ACCESSION   DATBFZ010000404 DATBFZ010000000
VERSION     DATBFZ010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 6724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6724
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1922692"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1652)
                     /gene="carB"
                     /locus_tag="VH419_12155"
     CDS             complement(<1..1652)
                     /gene="carB"
                     /locus_tag="VH419_12155"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEY7044413.1"
                     /translation="MGKRGDIRSVLVIGSGPIVIGQACEFDYSGTQACRVLSAEGVRV
                     ILVNSNPATIMTDPEVADATYVEPITPSLVAKVIDKERPDALLATLGGQTALNVAVAL
                     AEDGTLEKFGVELIGASIEAIEAGENRQSFKAIVESLGGEVARSVICHSTAECEAAAG
                     TLGYPLVVRPSYTMGGAGSGMAYDADDLRRIAGAGLQASPTTELLLEESVLGWKEYEL
                     EVMRDGHDNAVIVCSIENLDPMGVHTGDSITVAPAMTLTDREYQAMRDMAIGVIRAVG
                     VETGGCNIQFAVEPTTGRMIVIEMNPRVSRSSALASKATGFPIAKIAAKLALGYTLDE
                     IPNDITTRENGQSTPASFEPTLDYVVVKVPRFAFEKFPEADRRLTTHMKSVGEAMAIG
                     RNFTEALQKAMRSQELPHAGFSWSDDPGEASDLLVRAAVPHDGRLRLVMDALRAGATV
                     EEVCAATGIDPWFVDQLLLIHEVAMELQGAATLTATLLRKAKRHGFSDRQIAALCGSS
                     DEVVRGVRHALGVRPVYKTVDTCAAEFAAKTPYHYSSYDEET"
     gene            complement(1652..2929)
                     /gene="carA"
                     /locus_tag="VH419_12160"
     CDS             complement(1652..2929)
                     /gene="carA"
                     /locus_tag="VH419_12160"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEY7044414.1"
                     /translation="MTAGTPVAGRTEAVLVLEDGRTFRGEAYGALGETIGEAVFATGM
                     TGYQETLTDPSYHRQVVVMTSPHIGNTGVNDEDPESARIWVAGYVVRDPARRTSSWRS
                     RRSLDEELAAQGVVGISGIDTRALTRHLRERGAMRVGIASDGGGVDDLLARVRQAPSM
                     AGSALAAEVSTPTAYTVPAFGDRRFRVAALDLGIKAMTPRRMAERGIEVTVLPATARL
                     DDVLTTEPDGVFFSNGPGDPATADHEVDLLRGVLERRIPYFGICFGNQLFGRALGFGT
                     YKLGYGHRGLNQPVMDLTTGKVEVTAHNHGFAVDAPRDGKVETAYGVARVSHVCLNDD
                     VVEGLELRDEHGRLTAFSVQYHPEAAAGPHDAGYLFDRFVDLMSGTALIERVEISGVS
                     TDSSAASSEVANGIRHAGKNQRNLPPRRGAGEG"
     gene            complement(2926..4209)
                     /locus_tag="VH419_12165"
     CDS             complement(2926..4209)
                     /locus_tag="VH419_12165"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEY7044415.1"
                     /translation="MSTSYLIRGVRVLGGERADLVLKDGVIAEADDAAGKAVTIEADG
                     LVALPGLVDLHAHLREPGREDAETVESGSQAAALGGFTAVHAMANTEPVADTAGVVEQ
                     VLNLGQAAGYVDVRPVGAVTVGLAGEQLAELGAMADSAARVRVFSDDGRCVSDAVLMR
                     RALEYVKAFDGVVAQHSQEPRLTEDAQMNEGTMSGVLGMRGWPAAAEEAIIARDCLLA
                     AHVGSRLHVCHVSTAGSVELIRWAKSKGWNVTAEVTPHHLLLTDDLVATYDPVYKVNP
                     PLRTADDVAALRAGLADGTIDAVATDHAPHPTEDKECEWAAAAMGMLGLETALSVVQH
                     AMVDTGLLDWAGVADRMSVRPAAIGRLADHGGPLEPGAVANLVLFDPAATRVVDSTET
                     ASLSRNTPYAGTELPGRVVATFLRGRATVLDGKLQ"
     gene            complement(4206..5174)
                     /locus_tag="VH419_12170"
     CDS             complement(4206..5174)
                     /locus_tag="VH419_12170"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEY7044416.1"
                     /translation="MKRHLLSAADLTRDDALTILDTAAELRSLADRPIKKLPTLRGRT
                     VVNLFFEDSTRTRISFEAAAKRLSADVINFSAKGSSVSKGESLKDTALTLEAMGADAV
                     VIRHGASGAPHRLANSGWVRSCVVNAGDGTHEHPTQALLDAFTLRRHLGELDGRRISI
                     VGDVLHSRVARSNALLLATLGAEVTLVAPPTLIPVGVDSWPVQTSYDLDGALDKCDAV
                     MMLRVQRERMNAAFFPSAREYSRRYGLDERRFARLPEHAIVMHPGPMNRGMEIAANVA
                     DSARSVIVEQVTNGVAVRMAVLYLLLSGQDPLVATESGAGTEEAAR"
     gene            complement(5171..5731)
                     /gene="pyrR"
                     /locus_tag="VH419_12175"
     CDS             complement(5171..5731)
                     /gene="pyrR"
                     /locus_tag="VH419_12175"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007077628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEY7044417.1"
                     /translation="MDAGDIARALTRIAHEVLERNKGSDNVILLGIPSRGAPLARRIA
                     ATMSRVEGVDVPVGSLDITMYRDDLRLRPARALGATEIPPRGVDGAVVVLVDDVLFSG
                     RTIRAALDALHDVGRPRAVQLAVLVDRGHRELPIRADFVGKNLPTSLAERVSVCLVEY
                     DGAGSDATRDPVADSVVISTPEGGAS"
     gene            6058..6555
                     /locus_tag="VH419_12180"
     CDS             6058..6555
                     /locus_tag="VH419_12180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006131213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEY7044418.1"
                     /translation="MTENYAKALGMRLRAIRQQQGLSLQGVEEKSDGLWKAVVVGSYE
                     RGDRAVTVQRLSELAEFYGVPMNELLPGAHELGAAREAPPKLVLDIERLQQLDLNDAA
                     PLSRFTASIQSMRGDYNGRVLSIRQDDLRTLAVIYDEPPSSLAERLINWGVLRPDSRE
                     ATALS"
BASE COUNT          996 a         2345 c         2323 g         1060 t
ORIGIN      
        1 tcggtctcct cgtcgtacga cgagtagtgg tacggcgtct tagccgcgaa ctccgccgcg
       61 caggtgtcga cggtcttgta gaccgggcgt acaccgagcg cgtggcggac accacggacg
      121 acctcgtcgg agctgccgca cagggcggcg atctggcggt cggagaatcc gtgccgcttc
      181 gccttgcgca gcagtgtcgc cgtcagcgtc gcagcgccct gcagctccat cgcgacctcg
      241 tggatgagga gcagctgatc cacgaaccac gggtcgatcc cggtcgcggc gcacacctcc
      301 tcgaccgtcg cacctgcgcg gagcgcatcc atcaccaggc gcagccggcc gtcgtggggg
      361 acggccgcgc gtacgagcag gtccgaggcc tccccggggt cgtccgacca actgaacccc
      421 gcgtggggga gctcctgtga gcgcatcgcc ttctgcaacg cctcggtgaa gttgcgcccg
      481 atggccatcg cctcaccgac gctcttcatg tgcgtggtga gccggcggtc ggcctccggg
      541 aacttctcga acgcgaaccg cggcaccttg accacgacgt agtcgagcgt gggttcgaag
      601 cttgccggcg tgctctggcc gttctcccgg gtcgtgatgt cgttcgggat ctcgtcgagg
      661 gtgtaaccga gcgccagctt cgccgcgatc ttggcgatcg ggaacccggt cgccttggac
      721 gccagcgcgc tggaccgcga gacgcgcggg ttcatctcga tcacgatcat ccggcccgtg
      781 gtcggctcga ccgcgaactg gatgttgcag ccgccggtct cgacgcccac ggcgcggatg
      841 acgccgatcg ccatgtcacg catcgcctgg tactcgcggt cggtgagcgt catggcaggc
      901 gccaccgtga tcgagtcccc ggtgtgtacg cccatcgggt cgaggttctc gatcgagcag
      961 acgatgacgg cgttgtcgtg gccgtcccgc atgacctcga gctcgtactc cttccagccg
     1021 aggaccgact cctccagcag gagctcggtc gtcgggctcg cctgcaggcc agcgccggcg
     1081 atccgccgca ggtcgtcggc gtcgtacgcc atgccggacc cggccccgcc catcgtgtac
     1141 gacggtcgga cgaccagcgg gtatcccaac gtgcccgcag cggcctcgca ctcggccgtg
     1201 ctgtggcaga tgacgctgcg cgcgacctca ccgccgagcg actcgacgat cgccttgaac
     1261 gactggcggt tctcacccgc ctcgatcgcc tcgatggacg cgccgatgag ctcgacgccg
     1321 aacttctcga gggtcccgtc ctcggccagt gcgacggcga cgttgagtgc tgtctggcca
     1381 ccgagggtgg cgagcagggc gtccggccgt tccttgtcga tgaccttggc gaccaggctc
     1441 ggggtgatcg gctcgacata cgtcgcgtcg gcgacctcgg ggtcggtcat gatcgttgcc
     1501 gggttggagt tgacgaggat gacgcggaca ccctcggccg acaggacacg gcacgcctgg
     1561 gtgccggagt agtcgaactc gcatgcctgg ccgatcacga tcggccccga cccgatcacc
     1621 agcaccgacc tgatgtcgcc gcgcttcccc atcagccctc ccccgccccc cgccgaggtg
     1681 gcaagtttcg ttggtttttg ccggcgtgtc gtatgccgtt tgccacctcg ctggatgcgg
     1741 cgctcgagtc tgtcgagacc ccggagatct cgacacgctc gatcagcgcg gttccgctca
     1801 tcaggtccac gaaccgatcg aagaggtagc cggcgtcgtg cggtccggcc gcagcctccg
     1861 ggtggtactg cacgctgaac gccgtcagcc ggccgtgctc gtcacgcagc tccagcccct
     1921 cgaccacgtc gtcgttgagg cagacgtggc tgacccgtgc gacgccgtac gccgtctcga
     1981 ccttgccgtc gcgcggagcg tcgaccgcga aaccgtggtt gtgggcagtc acctcgacct
     2041 tgccggtggt gaggtccatc accggctggt tgagaccgcg atggccgtag ccgagcttgt
     2101 aggtgccgaa gcccagtgcc ctgccgaaca gctggttgcc gaaacagatc ccgaagtacg
     2161 ggatccgccg ctccagcacg ccacgcagca ggtcgacctc gtggtcagcg gtggccgggt
     2221 cgcccgggcc gttcgagaag aacacgccgt ccggctcggt cgtcagcacg tcgtcgagcc
     2281 gcgcggtcgc gggcagcacg gtcacctcga tgccacgctc ggccatccgt cgtggcgtca
     2341 tcgccttgat cccgaggtca agggcggcca cgcggaaccg tcggtcgccg aaagcgggca
     2401 ccgtgtacgc cgtcggcgtc gacacctccg ccgccaacgc agagccggcc atgctcggag
     2461 cctgccggac ccgcgccaac aggtcgtcga ccccgccgcc gtccgaggcg atgcctaccc
     2521 gcatcgcgcc acgctcgcgc aggtggcggg tgagcgccct cgtgtcgatg ccggagatac
     2581 cgacgacccc ctgcgcggcc agctcctcgt cgaggctgcg ccgtgaccgc cacgagctgg
     2641 tacgccgtgc cgggtcgcgc acgacgtacc ccgccaccca gatccgcgca gactccgggt
     2701 cctcgtcgtt gacgcctgtg ttgccgatgt gcgggctcgt catcaccacg acctggcggt
     2761 ggtacgacgg atcggtcagc gtctcctgat accccgtcat ccccgtggcg aagaccgcct
     2821 ccccgatggt ctcgcccagc gcgccgtagg cctcgccacg gaaggtgcgc ccgtcctcga
     2881 ggacgagcac ggcctcggtt cgcccggcca cgggggtgcc tgcggtcact ggagcttccc
     2941 gtcgagaact gtcgcacgac cgcgcaggaa cgtcgccacc acacgtcccg gcagctccgt
     3001 accggcgtac ggcgtgttgc gcgagagcga agccgtctcg gtggaatcga cgactcgcgt
     3061 ggcagccggg tcgaagagca cgaggttcgc gaccgcaccg ggctcgagtg gcccgccgtg
     3121 gtcggcaagg cgtccgatgg cggccggacg caccgacatc cggtcggcca cgccagccca
     3181 gtcgagcagg ccggtgtcga ccatggcgtg ctggacgacc gacaacgccg tctccaggcc
     3241 gagcatgccc atggccgccg cggcccactc gcactccttg tcctcggtgg gatggggcgc
     3301 gtggtcggtg gcgactgcgt cgatggtgcc gtcagccagc ccggcgcgca gtgccgcgac
     3361 gtcgtcagcc gtccggagcg gcgggttgac cttgtagacc gggtcgtacg tcgcgacgag
     3421 gtcgtcggtg agcagcaggt ggtgcggcgt gacctcggcg gtgacgttcc aacctttgct
     3481 cttggcccag cggatcagct cgaccgaacc ggccgtcgag acgtggcaga cgtgcagccg
     3541 cgaaccgacg tgcgccgcga gcaggcagtc ccgggcgatg atcgcctcct cggcagccgc
     3601 cggccaaccc cgcatgccga gaacgccgga catcgtgccc tcgttcatct gcgcgtcctc
     3661 ggtcagccgc ggttcttgcg aatgttgggc aaccacaccg tcgaacgcct tcacgtactc
     3721 cagtgctcgt cgcatcaaca ccgcgtcgga gacgcatcgc ccgtcgtcgg agaagacccg
     3781 gacccgggct gcggagtcgg ccatcgcacc gagctcggcc agctgctcgc cagccaaccc
     3841 gacggtgacg gcgccgaccg gacgcacgtc gacgtacccg gcggcctggc cgaggttgag
     3901 cacctgctcg acgacaccgg ctgtgtcggc aaccggctcg gtgttggcca tcgcgtggac
     3961 cgcggtgaag ccgccgagcg cggccgcctg cgacccggac tcgaccgttt cggcgtcctc
     4021 gcggcccggc tctcggaggt gggcgtgcag gtcgaccagg ccgggcagcg cgacgagtcc
     4081 gtcggcttcg atcgtgacgg ccttgccggc ggcgtcgtcg gcctcggcga tcacgccgtc
     4141 cttcagcacg aggtcggccc gctcgccacc gagcacgcgg acgcccctga tcaggtagct
     4201 cgtgctcatc gggcggcctc ctcggtcccg gcgccggact ccgtcgcaac cagcggatcc
     4261 tggccggaca gcagcaggta gagcacagcc atccgcaccg cgacgccgtt cgtgacctgt
     4321 tccacgatga ccgacctggc cgagtcggcg acgttggccg cgatctccat accgcggttc
     4381 atcggtccgg gatgcatcac gatcgcgtgc tcgggcagcc gggcgaagcg ccgctcgtcc
     4441 agcccgtacc gtcggctgta ctcacgggca ctcgggaaga acgcggcgtt catgcgttcg
     4501 cgctggacgc gcagcatcat gaccgcgtcg cacttgtcga gtgcgccgtc gaggtcgtac
     4561 gacgtctgga ccggccagct gtcgacgccg accggaatca gggtcggcgg cgcgaccagc
     4621 gtcacctcgg ctccgagggt cgcgagcagc agggcgttcg aacgggcgac gcggctgtgc
     4681 agcacgtcgc cgacgatgct gatccgccgg ccgtccagct cccccaggtg gcgtcgcagc
     4741 gtgaaggcgt cgagcagcgc ctgggtcggg tgctcgtgcg taccgtcccc ggcgttcacg
     4801 acgcaggacc ggacccagcc ggagttcgcc agccggtgcg gcgcacccga ggcgccgtgc
     4861 cggatgacga ccgcgtccgc ccccatcgcc tccagcgtca gcgccgtgtc cttgaggctc
     4921 tcgcccttgg agacgctcga gcccttcgcg gagaagttga tgacgtcggc cgaaagccgc
     4981 ttcgcggccg cctcaaagga gatgcgggtc cgcgtcgagt cctcgaagaa caggttgacg
     5041 acggtgcggc cccgcagtgt cggcagtttc ttgatcggac ggtcggcgag cgaccggagc
     5101 tcggcggcgg tgtcgaggat cgtcagcgcg tcgtcgcgcg tcaggtcggc ggcggacaac
     5161 aggtgacgct tcatgaggct cccccctcgg gtgtggagat gacgacggag tcggcgacgg
     5221 gatcccgggt ggcgtcggag cctgccccgt cgtactcgac gaggcagaca ctgacgcgtt
     5281 cggccagcga cgtcgggagg ttcttgccga cgaagtcggc acggatcggg agctctcggt
     5341 gtccccggtc caccagtacc gcgagctgga cggctctcgg acggccgacg tcgtgcagcg
     5401 cgtcgagtgc cgcccggatg gtcctgccgc tgaacaagac gtcgtcgacc aggaccacga
     5461 ccgcaccgtc gacgccgcgg ggcgggatct cggtggcgcc gagcgcccga gcgggacgca
     5521 gccgcagatc gtcgcggtac atcgtgatgt cgagggagcc gaccgggacg tccacgccct
     5581 cgacccggct catggtcgcg gcgatgcgtc gggccagggg tgcgccgcgg gacgggatac
     5641 ccaggaggat cacattgtcc gaacccttgt tgcgttcgag gacttcgtgg gcgatgcggg
     5701 tcaacgcgcg ggcgatgtct ccggcatcca ggacgacgcg ggagacttct gatccgccgg
     5761 atcgccgggg ggaaggggat gcgccggaac cggcgcttgc tgtggtggct gaagagccga
     5821 gttgggcaga cgggctgtca tggacagacg gcacaggcct gacctccttc tccgcctcac
     5881 gggacggctc gttaaaggat gtcttcgccc gtcaccgtat cagtggccgg cggcgcaaca
     5941 ggacatcacc cacggcattt tccgcaactc tgcgtaatcg caccgggtgc gtgggtacga
     6001 tcgtgcccga agtgccggac gacggtggtg gttcgtccga ccgtgaaggg acgaaccgtg
     6061 acagagaact acgcgaaggc gctcggaatg cggctgcgcg ccatccgcca gcagcagggt
     6121 ctttccctgc agggcgtgga ggagaagtcc gacgggctct ggaaagccgt cgtggtggga
     6181 tcgtacgaac gtggcgaccg cgccgtgacc gtccagcggc tctcggagct ggcggagttc
     6241 tacggcgtac ccatgaacga gctgctgccc ggtgctcacg agctcggagc ggcccgcgag
     6301 gcaccgccga agctcgtcct cgacatcgag cgtctccagc agctcgacct caacgacgcc
     6361 gcaccgctgt cgcgattcac cgccagcatc cagtcgatgc ggggtgacta caacggccgg
     6421 gtcctgtcga tccggcagga cgacctgcgc acgctggccg tcatctacga cgagccgccg
     6481 agctcgctgg ccgaacgcct catcaactgg ggtgtgctgc gtccggacag cagggaagcc
     6541 accgcgttga gctgaacccg gtttgccccg caccgccaaa cgggagtgtg tcggtggttt
     6601 gcctgggtag ccatccagac gcatgggttg ccaggcaggt ctgagtattc ctcaggtttc
     6661 tatggggcgg agtcggtcgt gatgggcggg ccgggtcgcc cgatcctccg tccgccgcgt
     6721 ctga
//
LOCUS       DATBFZ010000405         6252 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_1924387, whole genome shotgun sequence.
ACCESSION   DATBFZ010000405 DATBFZ010000000
VERSION     DATBFZ010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 6252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6252
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1924387"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(24..989)
                     /locus_tag="VH419_12185"
     CDS             complement(24..989)
                     /locus_tag="VH419_12185"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEY7044419.1"
                     /translation="MIADASDGIAVDSPAPSETHGGGNEATLVAEGICWTTVAGLAFI
                     HVGAGAGLAYLLTRPAAATLLLAVVLYVLCGLSITAGYHRLFAHRTYRASAPVRWFYL
                     AFGAATFQNSALAWSADHRAHHADTDGAGDPHAVGRGPWWVHVGWLFHRRQASADVSK
                     LGDLYAVRSIRLQHRFYPVLAIGLGLVLPMTIAWLFWGDPWGGLLVAGLLRAALMLQA
                     TFCVNSLAHMIGKPRYDRRVSARDSVVTALVTFGEGYHSFHHRFPYDYRNGIHWWQYD
                     PSKWLIWSLARLRLASRTRTASRASVGRARGRALDPLVAVKAPTG"
     gene            complement(1075..1515)
                     /locus_tag="VH419_12190"
     CDS             complement(1075..1515)
                     /locus_tag="VH419_12190"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEY7044420.1"
                     /translation="MELSERLSDRDLEFLTGNHTAGMITIAPDGTPKVARVGVAVVDG
                     KLWSSGTLDRTRTKRLRRDPRCTLYVTDPGFGWLALETTVTILDGADAPANNLRLMRV
                     MQGKPSGSLSWFGGELTEEEFLRTMVEERRVVYEFDVSRAYGMH"
     gene            complement(1549..4863)
                     /locus_tag="VH419_12195"
     CDS             complement(1549..4863)
                     /locus_tag="VH419_12195"
                     /inference="COORDINATES: protein motif:HMM:NF020701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucodextranase DOMON-like domain-containing
                     protein"
                     /protein_id="HEY7044421.1"
                     /translation="MPRRIGHLVAVGGVFVLGLGVVPLDAASGAAPLVAPSDGPGALS
                     HFDLARKNCLGTARNRRSKVWYTVANGVLSDVYYPTVDNTNVETLQYLVTDGSTFTDL
                     QSRNTTPAAEPLDAAGMICRVTSTANNGRYRIITDYVTDPDHNTLLMNVQLVPGPTPS
                     STPLQLYVRFDPTVNGNGGGGAGNGGGDSAVTDTSRGHPIPVAFDTVTTTNAANRDYA
                     QPVYAALDGPFSQVTSGFAGAPSDGLTQLDASHALTSVFDSASNGNIVQVAKVNLASS
                     SASAAGGATATLGLGFGATQAGAVRDAEASLATKLSKVEQSYEKDWTAYDAGLAKPPK
                     KLPGLSNARVAELDREYYLSANIVKASEDKTFPGAIVAGLASPWGQAVSAGDPNNTYF
                     GSYREVFARDLYEAWTGLMADGDRSTARDATLFLFNRQQLADGSMPRNSLVNGKVAPD
                     SFNTQLDETAYPLVMANDLGLTSSGLYTKHIKPAANFLIAHGPSFGPERWEEQSGYSP
                     STIAAEIAGLVAAADIADHNHDQVSARVWRAVADDFQRSIKSWTVTTTGSKANHPYFI
                     RLSKTGDPNAAITYNVGNGGPTLDQRDVIDAGFLELTRLGELRANDPDVLQSLPVVDA
                     TIKSTTNSGPGWHRYNGDGYGDGATDGHPWAPSDKGTGHLWPVLSAERGEQSLQTAGQ
                     ADAIALLDGMARFTSGIGILPEQDWELPNLAASPFGTDPTTASIGFVNGKAAGSASPL
                     TWSAGSFVRLFADIGANKLLDQPKDTVDRYITHTQGQTTLTVTAPADVSSVPGSPVTV
                     TGTSTPGNRIDVLATNTDQDFATTSASTTALADGSFSVDVPITGGTTVLSIAATSATH
                     ATAHATRTVVFDFVPGKLLFEVTDPDNDDNGPGNYAYPTSDNFHAGAFDLEDFQVYDD
                     GTNIIFRAKTRDLSPTFGSPLGAQLLDVYVHDPAAASTSTAASFQERNYSIADGSAWS
                     RLIEVQGFGQRYVDAGGTTLGTVQISANQVSRFITFSVPKSSLGTPASGWAFTVVLTG
                     QDGFSPDQARGFQATPQDFQFGVCATVSSDPHCTVDPGTVPKAVDVLTPAGVAQSDEL
                     DYTQHKPVTIQGVVMP"
     gene            5069..5911
                     /locus_tag="VH419_12200"
     CDS             5069..5911
                     /locus_tag="VH419_12200"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017936395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY7044422.1"
                     /translation="MLVCQGRAVADGRLAPNRFRDPTASAFLRTSEAIPVAQVRAGDP
                     PSGFGERIEFEMVQASAEVMVPRTVAIDDALREHVTPQLVILGAGLDGRAWRIDDLAS
                     VEVFEVDHPASQRDKRGRVADLPTHAKSLHFVPVDFTRDDLDGALESAGHQETVPTTW
                     IWEGVVPYLTRTEVDATVRVVADRSDRGSRLIVNYQTPSLSAAAGRAFARALALIGRR
                     PDPLAREARRSSWTPEAMRARLGRYGLHVVRDTDLLTLAERLPMAVRHRRSLRTGHIA
                     VADR"
     gene            6092..>6252
                     /locus_tag="VH419_12205"
     CDS             6092..>6252
                     /locus_tag="VH419_12205"
                     /inference="COORDINATES: protein motif:HMM:NF020921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044423.1"
                     /translation="MSAPLTDDELDLIDRYWRAANYLTVGQIYLQENPLLREPLAAEH
                     IKPRLLGHWG"
BASE COUNT          984 a         2052 c         2215 g         1001 t
ORIGIN      
        1 acgtaccccg cgtagggcgc ttcctaaccg gtcggagcct tcaccgccac caacgggtcg
       61 agcgcacggc cgcgggcgcg cccgaccgag gcccgtgaag cggtccgggt gcggctggcc
      121 agccgtaagc gcgcgagtga ccagatcaac cacttgctcg ggtcgtactg ccaccagtgg
      181 atcccgttgc ggtagtcgta cgggaagcgg tggtggaagc tgtggtaccc ctcgccgaac
      241 gtcacgagcg cggtcaccac gctgtcccgg gccgacaccc gacggtcgta gcgcggcttg
      301 ccgatcatgt gggcgagcga gttgacgcag aacgtcgctt gcagcatgag ggcggcgcgg
      361 agcagtccgg cgacgagcag gccaccccag ggatcgcccc agaagagcca ggcgatcgtc
      421 atcggcagga cgagtcccaa cccgatcgcc agcacagggt agaagcggtg ttgaagacgg
      481 atgctgcgca ccgcgtagag atcgccgagc ttggagacgt cagccgacgc ctgacggcga
      541 tggaagagcc agccgacgtg cacccaccac ggaccacgcc ccacggcgtg cgggtccccc
      601 gcgccgtctg tgtcggcatg gtgggcgcga tggtcggcgc tccacgcgag ggcggagttc
      661 tggaacgtgg cggcgccgaa ggcgaggtag aaccaacgca ccggcgccga ggcccggtac
      721 gtgcgatggg cgaagaggcg gtgatagccg gccgtgatcg acaggccgca caggacatag
      781 agcaccacgg ccagcagcag ggtcgcggcc gcgggtcgcg tcaacaggta cgcgagtcca
      841 gcaccggctc cgacgtggat gaacgccaag ccggcgaccg tggtccagca gatgccctcg
      901 gcgaccagcg tcgcctcgtt cccgccaccg tgggtctccg atggggccgg cgagtcgacg
      961 gcgatcccgt cgctcgcgtc ggcgatcacc cgggccgcaa ttcgaaggtc accctcagca
     1021 tcgggcgagt gtaggaggtc ggcgagtcgc gcaggcggcg cgcgctcggt tgggtcagtg
     1081 catcccgtag gcccgactga cgtcgaactc gtagaccact cgccgttcct cgaccatcgt
     1141 ccggaggaac tcctcctcgg tcagctctcc accgaaccag ctgagcgagc cactcggctt
     1201 gccctgcatg acccgcatga gcctcaggtt gttcgctggc gcgtcggcac cgtcgaggat
     1261 ggtcaccgtc gtctcgagcg cgagccaccc aaacccgggg tcagtgacgt agagggtgca
     1321 acgcggatcc cggcgcagcc gcttcgtgcg tgtccggtcg agcgtcccgc tgctccacag
     1381 cttcccgtcg acgacagcca cgccgacgcg cgccaccttg ggcgtgccat ccggcgcgat
     1441 cgtgatcatg cccgcggtgt gattgccggt caggaactcg aggtcgcgat cggacaggcg
     1501 ttcggagagc tccatgccgg cagcctatgg gcctgtcatg aatgggcgtc acggcatgac
     1561 gacaccctgg atcgtgaccg gcttgtgctg ggtgtagtcg agctcatcgg actgcgcgac
     1621 gccggcgggc gtgaggacgt ccacggcctt cggaacggtg ccggggtcga ccgtgcagtg
     1681 cggatcgctg ctgacggtgg cgcacacgcc gaactggaaa tcctgtggtg tggcctggaa
     1741 accgcgggcc tggtcgggac tgaagccgtc ctgacctgtg agcacgacag taaaagccca
     1801 ccccgaggct ggcgtgccga gtgacgactt gggcacgctg aaggtgatga accgcgacac
     1861 ctggttcgcg ctgatctgga ccgtcccgag ggtggtgccg ccggcgtcga cgtagcgctg
     1921 cccgaacccc tgcacctcga tgagtcgact ccaggccgag ccgtcggcga tcgagtagtt
     1981 ccgctcctgg aacgaggccg cggtggaggt cgaagcagca gccgggtcgt gcacgtacac
     2041 gtccagcaac tgggcgccca gcgggctccc gaacgttggt gacaggtcgc gcgtcttggc
     2101 ccggaagatg atgttggtgc cgtcgtcgta gacctggaag tcctcgaggt cgaaggcacc
     2161 ggcgtggaag ttgtcggagg tcgggtaggc gtagttgccg gggccgttgt cgtcgttgtc
     2221 gggatcggtg acctcgaaga gcagcttgcc gggcacgaag tcgaacacga cggtccgggt
     2281 ggcgtgcgcg gtggcgtgag tcgccgaggt cgcggcgatg ctgaggacgg tggtaccgcc
     2341 cgtgatgggc acgtccactg agaacgatcc gtcagcaagc gcggtcgtgc tggccgacgt
     2401 cgtcgcgaag tcctgatccg tgttcgtcgc cagcacgtcg atccggttgc ccggcgtgct
     2461 cgtcccggtc accgtcacgg gcgaacccgg caccgacgac acgtccgccg gagcggtcac
     2521 ggtcagtgtc gtttggccct gcgtgtgggt gatgtaccgg tcgacggtgt ccttcggttg
     2581 atcgagcagc ttgttggcgc cgatgtcggc gaacaggcga acgaacgagc cggccgacca
     2641 cgtcagcggc gacgccgatc cggcggcttt gccgttcacg aacccgatcg acgccgtggt
     2701 cgggtcggtc ccgaacggcg acgcagccag gttcgggagc tcccaatcct gctcgggcag
     2761 gatgccgatg cccgacgtga agcgagccat gccgtcgagc agcgcgattg cgtccgcctg
     2821 gccggcggtc tggagcgact gctcgccgcg ctcggccgac aggacgggcc aaagatgccc
     2881 cgtccccttg tcgctgggcg cccacgggtg cccatcggtc gcgccgtcgc catagccgtc
     2941 gccgttgtag cggtgccagc ccgggccgct gttcgtcgtt gacttgatcg tcgcgtccac
     3001 cacgggcagg gactgcagga cgtcgggatc attggctcgg agctcaccga gccgggtcag
     3061 ctcgaggaag ccggcgtcga tgacgtcgcg ctgatcgagc gtcggtcccc cgttgccgac
     3121 gttgtacgtg atcgccgcgt tgggatcacc tgtcttcgac aggcggatga agtaggggtg
     3181 gttggccttc gaaccggttg tggtcaccgt ccacgacttg atcgagcgtt ggaaatcgtc
     3241 ggcgacggcc cgccacaccc tcgccgagac ctggtcgtga ttgtggtcgg cgatgtccgc
     3301 ggcggctacg agccccgcga tctctgccgc gatggtcgac ggcgagtagc cggactgctc
     3361 ctcccaccgc tccggaccga acgacgggcc gtgggcgatg aggaagttgg cggccggctt
     3421 gatgtgcttg gtgtagagcc cagagctcgt caggccgagg tcgttcgcca tgacgagtgg
     3481 gtacgcggtc tcgtccagtt gtgtgttgaa cgaatccggc gcgaccttgc cgttgacgag
     3541 gctgttccgc ggcatcgagc cgtccgccag ttgttgccga ttgaacaaga agagggtggc
     3601 gtcgcgcgcc gtgctgcgat cgccatccgc catgaggccg gtccaggcct cgtagaggtc
     3661 gcgggcgaac acctcgcggt acgacccgaa gtaggtgttg ttcggatcgc cagcggacac
     3721 cgcctgaccc cacggcgacg ccaatccggc gacgatggca ccagggaacg tcttgtcttc
     3781 ggacgccttc acgatgttcg cgctcaggta gtactcgcga tcgagctccg ccacccgggc
     3841 attggagagg ccaggcaact tcttcggcgg cttcgccaga ccggcgtcgt aggccgtcca
     3901 gtccttctcg tagctctgtt cgaccttgga gagcttggtg gccagcgacg cttcggcatc
     3961 gcggacggca ccagcctgcg tggcaccgaa cccgaggccg agcgtggccg ttgccccgcc
     4021 ggcagccgaa gcggacgacg aggccaggtt gaccttcgcc acctgcacga tgttgccgtt
     4081 gctggccgag tcgaagaccg acgtgagcgc atgcgaggcg tcgagttggg tcagaccgtc
     4141 gctcggggct ccagcgaagc cgctcgtgac ctgggagaac ggtccatcca gagcggcgta
     4201 gacgggctgg gcgtagtcac ggttcgccgc gttggtcgtc gtcacggtgt cgaaggcgac
     4261 cgggatcggg tggccgcgcg aggtgtccgt gaccgcggaa tcgccaccgc cgttacctgc
     4321 accaccacct ccgttgccgt tcaccgtggg atcgaagcgc acgtacaact ggagaggcgt
     4381 gctgctcggc gtgggtccgg gaacgagctg cacgttcatc agcagcgtgt tgtggtccgg
     4441 atcggtgacg tagtccgtga tgatccgata gcggccgttg ttggcggtgc tggtgacccg
     4501 gcagatcatc cccgcagcgt cgagcggctc cgccgccggt gtcgtgttgc gcgactggag
     4561 atcggtgaag gtggaaccgt cggtgacgag gtactgcagc gtctcgacgt tcgtgttgtc
     4621 gaccgtcggg tagtagacgt cgctgaggac gccgttggcc accgtgtacc agaccttgga
     4681 gcgccggttg cgagcggtgc cgaggcagtt cttgcgggcc aggtcgaagt gcgacagcgc
     4741 cccgggtcca tccgacggag cgacgagtgg cgcggcgcca cttgctgcgt ccagcggcac
     4801 gaccccgagt ccgagcacga acacgcctcc gacggcgacg aggtgaccga tccgacgagg
     4861 catccgccgg ttcatccggc gacgcgacca ggcatgcatt gcagttctcc ccccgcccgc
     4921 tgttcctgcg tgcagcgtgg atcgcgcccg ggcgagtgtc aagcggggcg tccagtgcag
     4981 ccgccaaatg accacactcc gttgtctacg ggcgattggc gcgccaggct tgatcgcagt
     5041 ggatgccacc gaagcgagcc gcacggccgt gctcgtgtgc caaggccggg cggtggctga
     5101 cgggcgcttg gcgccgaacc ggtttcgcga cccgaccgcg agcgcgttcc tgcgcacgtc
     5161 ggaagccatt ccggtcgcgc aggtgcgagc aggcgacccg cccagcggct tcggggagcg
     5221 gatcgagttc gagatggtgc aggcctcggc cgaggtcatg gtgccgagga cggtcgccat
     5281 cgacgacgcc ctccgtgaac atgtgacgcc gcagctggtg atcctcgggg ccggactcga
     5341 cggccgtgcg tggcgaatcg acgacctggc cagcgtcgag gtcttcgagg tggaccaccc
     5401 cgcctcacag cgcgacaagc gcggtcgggt cgccgatctg ccgacgcatg cgaagtcgtt
     5461 gcatttcgta ccggtcgact tcacgaggga cgacctcgat ggcgcgctgg agtcggcggg
     5521 ccatcaggag accgtgccga ccacatggat ctgggaaggc gtcgttccgt acctgacgcg
     5581 aacggaggtc gatgccacgg tgcgggtcgt cgccgatcgg tccgaccgcg gcagccggct
     5641 catcgtcaac taccagacgc cgtcgctgtc ggccgccgcc ggtcgtgcgt tcgcgcgagc
     5701 gctggcgctc atcggccggc gccctgaccc gctcgcccgt gaggcccgac ggtcctcgtg
     5761 gacgcccgag gcgatgcgag cgaggctcgg ccggtacggc ctccatgtgg tgcgggacac
     5821 cgacctcctg acgctcgccg agcggctgcc catggcggtg cgccatcggc gctcgctgcg
     5881 caccggccac attgcggtag cggaccgcta gcacacgcgt cgccggcagg ggcaggaaac
     5941 gggcgtggat gcactcgcaa ctgctgccag catctgaccg cgcaccgccg gtttcgacga
     6001 cgttcacctg cgggcaacct cgaacgacga cgatcaaggc gccgcagccc cgcgccttag
     6061 gctgcccacg aacctacgac gggaggcgcc catgtccgcg ccgctgaccg acgacgagct
     6121 cgatctcatc gaccgctact ggcgcgccgc caactatctg acggtcggcc agatctacct
     6181 ccaagagaac ccgttgctac gggaaccact ggccgccgag cacatcaagc cccgcctgct
     6241 cggccactgg gg
//
LOCUS       DATBFZ010000406         3953 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_2906385, whole genome shotgun sequence.
ACCESSION   DATBFZ010000406 DATBFZ010000000
VERSION     DATBFZ010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 3953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3953
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2906385"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="VH419_12210"
     CDS             <1..335
                     /locus_tag="VH419_12210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044424.1"
                     /translation="LRGALADQLNNVVAQNEGYGVSRLAPQPALQPHIMQPPNARKCR
                     WSRASPSISGTQRIPFDRTYEQVTPTEGSALADENSTLAPAGFWIKVSIGRVDSGRLV
                     KGQSCRTR"
     gene            complement(399..1484)
                     /locus_tag="VH419_12215"
     CDS             complement(399..1484)
                     /locus_tag="VH419_12215"
                     /inference="COORDINATES: protein motif:HMM:TIGR02607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HEY7044425.1"
                     /translation="MTAALAEVFPVGEMLADELEARGWSQVEFAEILGRPTQFVSEIV
                     NGKKEITRESAAQIAAALGTSAELWLGMQDQFLLAEQAKDAGTQTRLSEVRVRALISK
                     HAPVELLRKRGILTANNLNDLAKQVKDLYELTSLEDEPAFLVAARRANKDEDLSILQT
                     TWFACVRRQARRQPPVKAYSANKLTTLARRLSQTLHLAEDFQGLPDRFREAGVRLVFV
                     DAFPSAKIDGGSMYVDGYPVIGLSGRGKRLDKVLFTLLHEIGHILKRHVDEHHYIVEE
                     IVETHDEQGVQESEADNLASDLLFQSAFPQVPARIGGPWVDQVANQLDVAPIVVVGHL
                     QHKRRLDWRTTLAKNAPVVGDALETWA"
     gene            complement(1499..1795)
                     /locus_tag="VH419_12220"
     CDS             complement(1499..1795)
                     /locus_tag="VH419_12220"
                     /inference="COORDINATES: protein motif:HMM:NF016875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEY7044426.1"
                     /translation="MIEYHDDELRRLAEDPNFRPRQWGRDVVVAYRKKIQILEGAKDE
                     RDLYAMQSLNLEKLKGKRTGQSSIRLNDQFRLILTFRTGDDGRVVVVLELVDYH"
     gene            1993..2235
                     /locus_tag="VH419_12225"
     CDS             1993..2235
                     /locus_tag="VH419_12225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044427.1"
                     /translation="MSELHDRKKITFTIDGMKYSTHDDDQEASALLRLAGVDPAQYDL
                     AKIKHNGETKIYRDEKVIDLKDGEAFVTVRQSAPVA"
     gene            2235..2603
                     /locus_tag="VH419_12230"
     CDS             2235..2603
                     /locus_tag="VH419_12230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044428.1"
                     /translation="MSADPTPVDGVSRFVADLEDEGCEPTVSGGVVSYNVVPATGARA
                     GQKVPTGVSASELQGWPMTPPHWIHLRDDITFTQTNSDTQDCPPGWHRHSRDTGLWDM
                     SRRPIHLWISHVRGVIGEAV"
     gene            2605..>3953
                     /locus_tag="VH419_12235"
     CDS             2605..>3953
                     /locus_tag="VH419_12235"
                     /inference="COORDINATES: protein motif:HMM:NF013094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HEY7044429.1"
                     /translation="MRATSVAMTQDVHDELAAHLLRADRQEDICIATYTVSTGASRTT
                     RILHEVHRPADGDRLVHGNATINGTYVLRVAALASQRGHGIAILHSHPGGRGWQQLSS
                     ADADAESSYARLAEQVTKLPLVGLTLAGDTNWSARTWRDGQPLWAGSVRRVGKILTVS
                     WNDEMVPAPTPEASQERTISAWGANLHRDIVRLRVLVVGVGSVGLDVVQRLAATGIIE
                     LGVMDFDRVDEVNRDRMIGATRRDARLRRRKVDVAARLARLAATAGDFNVHFHHTSVC
                     SPAGQAAALDYDVIFSCVDRPWPRAVLNSLAYADAIPVIDGGIAIDTFDTGGMRGATR
                     RTQIATPGAPCLSCSGQIEMSEVTLEMSGDLDNPNYIRRAGREHVSGRPNVAALCAGV
                     SASQLELFVSLVAHPAGLGTPVPLRFILAPHHLEHVDASVLPYCETELRTSAGDARI"
BASE COUNT          727 a         1346 c         1201 g          679 t
ORIGIN      
        1 atctgcgcgg cgctctcgcc gatcagctca acaatgtcgt cgcgcagaac gagggctacg
       61 gagtctcaag gctcgcgccg caaccggccc ttcaaccgca tatcatgcag ccgccgaacg
      121 cacgaaaatg ccgctggtcg cgcgcctcac cgtcgatatc gggcactcag cgtatcccgt
      181 tcgaccgcac atatgaacaa gtaacgccca ccgagggatc cgctcttgcc gatgagaatt
      241 cgacgctcgc cccggccggt ttctggatca aagtcagcat aggtagggtg gatagcggca
      301 ggcttgtgaa aggccaatct tgccggacgc ggtgaagcga cagagtagag gcaatatcgc
      361 tcccctggca cggctgatcg accgcgtagc aactgtggct aagcccacgt ctccagtgcg
      421 tcgcccacca ccggtgcatt ctttgccagt gtggttcgcc aatctagtcg acgcttgtgc
      481 tgcaagtggc ctacgacgac gatgggtgcg acgtcgagtt ggttcgcgac ctggtcgacc
      541 cacggcccgc cgatcctggc tgggacctgt ggaaatgcgg actgaaacag caggtcgctg
      601 gctaggttgt cagcctcgga ctcctgaacg ccttgctcgt cgtgggtttc gacgatctcc
      661 tcgacgatgt agtgatgttc gtcgacatgc cttttcagaa tgtgcccaat ctcgtgcagc
      721 agcgtgaaaa ggaccttatc taagcgctta cctcgtcccg acaggccgat cacggggtag
      781 ccgtcgacgt acatggaccc gccgtcaatt ttggctgacg ggaaggcatc gacgaagact
      841 aggcgcacgc cggcctctcg aaaccgatcc ggaagccctt ggaagtcctc cgcgaggtgc
      901 agcgtctgcg ataggcgcct ggccagggtc gtgagcttgt tggcgctgta ggccttaacc
      961 ggaggctgcc gtcgcgcctg cctgcggaca cacgcgaacc aggtggtttg aaggatgctc
     1021 aggtcctcgt ccttgttcgc gcggcgcgcc gcgactagaa acgccggctc gtcctccaac
     1081 gacgtgagct cgtacagatc tttgacctgt ttcgcgaggt cattcagatt gttagcggtg
     1141 agtatcccgc gtttgcggag caactcgaca ggggcgtgct tgctgatcaa agctcgaact
     1201 cggacctcgc tgaggcgagt ctgcgtgccg gcgtccttcg cctgctctgc caagagaaac
     1261 tggtcctgca tgccgagcca cagctcagca ctggtaccta gcgccgccgc gatctgggca
     1321 gccgactcgc gcgtgatctc cttcttgccg ttgacgatct cagagacgaa ctgagtcggt
     1381 cgcccgagaa tctccgcgaa ctcgacctga gaccacccgc gggcctccag ctcgtcggcc
     1441 agcatctcgc ccacggggaa cacctcggcc agggcagcag tcatggcgat gcctcctttc
     1501 agtgatagtc gaccagttcg agaaccacga caaccctgcc gtcgtccccg gtgcggaacg
     1561 tgaggatcag ccggaactga tcgttgagac ggatggagga ctgccccgtc cgtttgcctt
     1621 tgagcttctc gaggttcagg gactgcatcg cgtacaagtc gcgctcgtcc ttggcgcctt
     1681 cgaggatctg gatcttcttg cggtacgcga cgaccacgtc gcgtccccat tgccgtggcc
     1741 gaaagttggg atcttcggca agacgacgca gctcatcgtc gtggtactcg atcaccactc
     1801 ggattcactc catcacccaa cggccgatgt cgcgaaccgt accggtttgc cacttcattc
     1861 accgcgcgac ccgccgagcc aagtaagtct tcttgccatc accccttcac ccttgtagag
     1921 aagataatac tcaggttaga gtcgagatgc acctgccacc cggcggggca gaagagccac
     1981 aggaggtttg agatgagcga gctgcacgac aggaagaaga tcacgttcac gatcgacggc
     2041 atgaagtact ccacgcacga cgacgaccag gaggcctccg cactgctgcg cctcgccggc
     2101 gtcgaccccg cccagtacga cctggccaag atcaagcaca acggcgagac gaagatctac
     2161 cgggacgaga aggtcatcga cctgaaggac ggcgaggcgt tcgtgacggt ccgccagagc
     2221 gcaccggtcg cctgatgtcc gccgacccaa ctcccgtgga cggcgtgtcc cggttcgtcg
     2281 ccgacctcga ggatgagggt tgcgagccga ccgtgagcgg gggcgtcgtg tcgtacaacg
     2341 tcgtccccgc cacgggcgcc cgcgcaggac agaaggtccc caccggcgtc agcgcgagcg
     2401 agctccaggg ctggccgatg acaccgccgc actggattca tctccgcgac gacatcacct
     2461 tcacccagac caacagcgac acccaggact gccctcccgg ctggcaccgc cactcgcgcg
     2521 acaccggcct gtgggacatg agtcgccggc cgatccacct ctggatcagc cacgtccgcg
     2581 gcgtcatcgg agaggcggtc tgaggtgcgc gcgacctcgg tggcgatgac gcaggacgtt
     2641 cacgatgaac tcgctgccca cctgcttcgc gccgaccggc aggaggacat ttgcatcgct
     2701 acgtacaccg tctccaccgg cgcgagccgc actactcgca tcctgcacga ggtgcaccga
     2761 ccggccgatg gcgaccgcct ggtgcacggc aacgccacta ttaacggcac ctacgtcctg
     2821 cgggtcgcag ccctggcttc tcagcggggc cacgggatcg ccatcttgca cagccaccct
     2881 ggcgggcgcg gatggcagca gctcagcagt gccgacgccg acgccgagag ctcctacgct
     2941 cgcctggccg agcaagtcac caagctcccg ctggtcggtc tcacgctggc gggcgacacg
     3001 aactggtccg cccgcacgtg gcgcgacggg caaccgctct gggcgggctc cgtgcgccgg
     3061 gtcgggaaga tcttgaccgt ctcgtggaac gacgagatgg tgcccgcgcc cacgccggaa
     3121 gcgtcgcaag agcgaaccat ctccgcgtgg ggtgccaatc tgcaccgcga catcgtccga
     3181 cttcgagtgc tcgtggtcgg cgtcggaagc gtcggactcg acgtcgtgca gcggctcgcg
     3241 gcaaccggaa tcatcgagct cggcgtcatg gacttcgacc gcgtagacga ggtcaaccgg
     3301 gaccgcatga tcggcgccac ccgccgcgac gcgcgactgc gccggcgcaa ggtcgacgtc
     3361 gccgcccgct tggcgcgact cgcagccaca gccggcgatt tcaacgttca cttccaccac
     3421 accagcgtgt gcagccccgc aggccaagcc gctgcactgg actacgacgt catattcagc
     3481 tgcgttgacc ggccatggcc gcgcgccgtg ctcaactcgc tcgcgtacgc cgacgccatc
     3541 cccgtaatcg atggcggcat cgccatcgac actttcgaca ccggcggcat gcgcggcgcc
     3601 acccgccgaa cccagatcgc caccccgggc gctccctgcc tatcctgctc cggccagatc
     3661 gaaatgtccg aggtgactct ggagatgagc ggcgatctcg ataaccccaa ctacatccgg
     3721 cgcgccggca gggagcacgt ctcgggccgc cccaacgtcg cggctctgtg tgccggcgtc
     3781 agcgctagcc agcttgagct cttcgtcagc ctcgtcgcgc atcctgcagg actagggaca
     3841 ccggtcccgc tgaggttcat cctcgcgcca caccacctgg aacatgtcga cgcgtccgtg
     3901 ctgccgtact gcgagaccga gctacgcacc tcggccggag atgcgcgaat cga
//
LOCUS       DATBFZ010000407         3721 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_2255837, whole genome shotgun sequence.
ACCESSION   DATBFZ010000407 DATBFZ010000000
VERSION     DATBFZ010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 3721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3721
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2255837"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="VH419_12240"
     CDS             <1..410
                     /locus_tag="VH419_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEY7044430.1"
                     /translation="PFEVTVDLGGPDERVVDALADAEFVSAGQVIRIRTTGGGGWGDP
                     LERPYAEVARDVAWGKVSVEGARADYGVVVIRDEDTGVVDVDEAAADTLRRTLRETRA
                     AAIAAGAAEPFFDRGPGYARLSGGAPHSPLDIC"
     gene            718..1425
                     /locus_tag="VH419_12245"
     CDS             718..1425
                     /locus_tag="VH419_12245"
                     /inference="COORDINATES: protein motif:HMM:NF025687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HEY7044431.1"
                     /translation="MDLQRTGRIFGWLFIGTFVTSIPARLLFINGVGASWSDMRFVAG
                     DASTASIKWGAVLEFLLIVTQIGTAVVIFPLLRRQSETVSLGYVTARIMESVFAAIGL
                     VSMLSVVSVIGALDGASDTEATALETQGNSLVHTYEWAFEWGPGLVAGIGNGIMLGYL
                     MYRSGLVPRGMAVLGLIGGPLLIVSFVMILAGVYKNGDPLSGLLTLPEAAWELSLGIY
                     CAIWGFRRDSPIASTEP"
     gene            2142..2396
                     /locus_tag="VH419_12250"
     CDS             2142..2396
                     /locus_tag="VH419_12250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020385202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044432.1"
                     /translation="MSDSTRRGFLALVGAGAATAATAAAAPGSLAADDWAPKITSHRP
                     LVAYVTDPSKGRVSVMVGEREVVVHDRDLVARLLRAARKG"
     gene            2403..>3721
                     /locus_tag="VH419_12255"
     CDS             2403..>3721
                     /locus_tag="VH419_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020388739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4331 domain-containing protein"
                     /protein_id="HEY7044433.1"
                     /translation="MSSHREAPEISKDPVADNTDTYAFVSPDQRDTVTIIANFIPLQK
                     PDGGPNFYEFGEDVLYEIHISNRGDAESDITYQFRFNTKIRNPNTFLYNTGPITKLRD
                     QTWNRPQFYSVTRVDHHGTHRLGSGFKCPPVNIGPRSTPNYAHFTDLAVHDLPGGGKV
                     FAGQRAEGFHVDLGSIFDLGTLRPFQMLHLIPSAAAVGVNGTQALNVHSIAIQVPIRA
                     LTRHGNRPTDVMDADSVIGVWASASRQKTRILDHQTGLTQGFGPFHQVSRLAMPLFNE
                     VIVPMSRKDVWNAREPRNDDAFAKYVEKPELASLLPVLYPGVFPNLAAYKKPRADLSA
                     ILLTGLKPGVVSGFQNFTGPTKADMLRLNVAVPPSDNPKELGLVAGDPAGFPNGRRVF
                     DDTVTVELRAIAGLTIPLVDSSYTVDGAASQIQDGTTNTNSPYLNRFP"
BASE COUNT          601 a         1218 c         1253 g          649 t
ORIGIN      
        1 ggccgttcga ggtgaccgtc gacctcggtg gtccggacga gcgggtggtc gatgcgctgg
       61 cggatgcgga gttcgtgtct gctggtcagg tgatccggat caggacgacc ggtggcggcg
      121 gatggggcga cccgttggaa cgtccgtacg ccgaggtggc ccgtgacgtc gcctggggga
      181 aggtgtcggt cgagggcgcg cgggcggact acggcgtcgt ggtcatccgc gacgaagaca
      241 ccggtgtcgt cgacgtcgac gaggctgccg cggacaccct ccggcgcacc ctccgggaga
      301 cgcgagccgc ggcgatcgca gccggagcgg ctgagccgtt cttcgaccgt ggcccgggtt
      361 acgcccgtct ctccggcggc gccccccact ctcccctcga catctgctga cccttttccc
      421 gccgaggtgg caggggtgtc aggtgggtcc cgctcgtcgc gagcggggtc gaggtggatg
      481 agggttctaa ccggttgtgg tgaagcccgc gagcgtcgtc gagacaacgc tgcctgagac
      541 cggccgcctc gacgctcgcg gcccgccgct cctcctgatg gcttcttgcg gcgggcctcg
      601 accacgaacc cgcgccccga agaactgtcg gctttgtttg tttgacgcga cctacccgac
      661 ccggcagggt tgccgagacg atcctctgtg gggtcgtccg cacttcaagg ggagcccatg
      721 gaccttcaac ggactggccg gatctttggc tggcttttca tcggtacgtt tgtcacgagc
      781 atccccgctc gccttctctt catcaacggc gtgggggcga gttggagcga tatgcgcttc
      841 gtcgccgggg atgcctcaac cgcgagcatc aagtggggcg cggtcctcga gttcctcctg
      901 atcgtcactc aaatcggcac cgcagtcgtc atattcccgc tcctgcggcg gcagagcgaa
      961 acggtctcgc tcggatacgt caccgcccgc atcatggagt ccgtgttcgc cgcgatcggc
     1021 ctcgtcagca tgctctcggt cgtgtccgtc attggtgcgc tggacggggc gagcgacaca
     1081 gaagccacgg ctcttgagac gcagggcaac tctctcgtgc acacgtacga gtgggcgttc
     1141 gagtggggac cgggactcgt tgcgggcatc ggaaatggca tcatgcttgg ctacttgatg
     1201 tacagatcgg gacttgttcc ccgtggcatg gcggtgttgg gcctgatcgg cggtcccctg
     1261 ctgattgtgt cgttcgtgat gatcctggct ggcgtctaca agaatggaga tccgctctcg
     1321 ggcctcctga cccttccgga ggccgcatgg gagttgtcgc tcggcatcta ctgcgcgatc
     1381 tgggggttca gaagggatag cccgatcgcg tccacagagc catgacctcg gagccgtctg
     1441 cgggcagaac ccgacggggc cgaccgacgc actccccgcg cggcagcgcg tactcgctct
     1501 gtcgggcgtc gtattcgcag tgctcctgat tctcgcttgg ttcctgagcg gtggcgacgc
     1561 gccggactac acggcggccg acgcggactg gacgactggg ccgatgacaa ccgatccagg
     1621 agcggcgtcg ctggcttcct catcctcgtc gcagccgtcg cgttcttgca cttcgcaggg
     1681 tcagtgagac gcgcgctcgc cagtgatgag acgagagttc acaggtccga gggcggtcct
     1741 gataccagcg atgcgaacga gagcgttccg ggaatcctgg cactcgtgac cttgatgacg
     1801 catcgccgcc aggtgctccg gcccgatggc cgacacgtgt agtcctgccg cggccgatcg
     1861 ggagggtccc tgcgatgtgg tggtcgtggt tgtggtcgcc atggatcacc tctgaccgat
     1921 tgccgcgccc gactctgggg gttcggacat cgctgcacac gggcgtcgta cgccgaggtg
     1981 gcgatcggcg tccgacacgc cgacaaccat caaccaaacc tgccacctcg gcgggaaggg
     2041 agtgggcatc caagggtggg tgcggtgacg aaccagagac gttgaagacg cacgggtacg
     2101 ccatggtggc gtgccccggt cgacagggga gggagacttc catgagcgac tcgacacgac
     2161 gggggttcct ggcgctggtg ggcgccggtg ccgccactgc agcaaccgct gccgcagcac
     2221 ccgggagcct ggccgccgac gactgggccc cgaagatcac gtcccaccgc ccattggtgg
     2281 cctacgtgac cgaccccagc aagggccggg tctcggtgat ggtgggtgag cgagaggtcg
     2341 tcgtacacga ccgtgacctg gtggctcggc tgctccgtgc cgcgaggaag gggtgacctg
     2401 ccatgtcgtc acatcgcgaa gcaccggaga tctccaagga cccggttgcc gacaacaccg
     2461 acacctacgc gttcgtgagc cctgaccagc gcgacacggt cacgatcatc gcgaacttca
     2521 tcccgctgca gaagcctgac ggcgggccga acttctacga gttcggtgag gacgtgctct
     2581 acgagatcca catctccaac cgcggcgacg cggagtcgga catcacctac cagttccgct
     2641 tcaacacgaa gatccgcaac cccaacacgt tcctgtacaa caccgggccg atcaccaagc
     2701 tccgggacca gacatggaac cgtccgcagt tctactcggt gacccgggtc gaccaccacg
     2761 ggacccaccg gctgggcagc ggtttcaagt gtcctccggt caacatcggg ccgcgcagta
     2821 cgccgaacta cgcgcacttc accgaccttg cggtgcatga cctgccaggt ggcggaaagg
     2881 tgttcgccgg tcagcgcgcg gagggcttcc acgtcgacct cggcagcatc ttcgacctgg
     2941 gcacgctccg cccgttccag atgctgcacc tgatcccgtc cgcggctgcc gtgggcgtga
     3001 acggcacgca ggcgctgaac gtccacagca tcgcgatcca ggtcccgatc cgcgcactga
     3061 cgcggcacgg gaaccggccc acggacgtga tggacgcgga ctcggtgatc ggtgtctggg
     3121 cgagtgccag ccggcagaag acccgcatcc tcgatcacca gaccgggctg acgcagggct
     3181 tcggaccgtt ccatcaggtg tcccggcttg cgatgccgct gttcaacgag gtcatcgtgc
     3241 cgatgtcccg caaggacgtc tggaacgctc gtgagccgcg caacgacgac gcgttcgcca
     3301 agtacgtcga gaagccggag ctggccagcc tgttgccggt gctctacccc ggggtcttcc
     3361 cgaacctggc ggcgtacaag aagccgcgtg ccgacctgtc ggccatcctg ctgaccggtc
     3421 tcaagcctgg tgtggtgtcc gggttccaga acttcaccgg gccgacgaag gcggacatgc
     3481 tccgactgaa cgtcgccgtg ccgccgtcgg acaacccgaa ggagctcggc ctggtggccg
     3541 gcgacccggc ggggttcccc aacggtcgac gggtgttcga cgacaccgtg accgttgagc
     3601 tgcgggcgat cgccgggctg accatcccgt tggtcgactc gtcgtacacg gtggacggcg
     3661 ctgcttcgca gatccaggac gggacgacga acacgaactc gccgtacctg aacaggttcc
     3721 c
//
LOCUS       DATBFZ010000408         6357 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_618345, whole genome shotgun sequence.
ACCESSION   DATBFZ010000408 DATBFZ010000000
VERSION     DATBFZ010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 6357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6357
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_618345"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="VH419_12260"
     CDS             <1..234
                     /locus_tag="VH419_12260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044434.1"
                     /translation="CRRYRGSAASPLFLINHWLDRPHTLVSDAAKVNAYDVLWPRVEK
                     CQQQRALLPNYVAVNFYDKGDLLDVVDQLNELD"
     gene            329..1687
                     /locus_tag="VH419_12265"
     CDS             329..1687
                     /locus_tag="VH419_12265"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020578469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEY7044435.1"
                     /translation="MTSTSEETDLYGFPIVERMRDFDIPGVSIAVIEAGTVVEKRCFG
                     VRTSTDPSPVTESTRFQACSISKPVCSLAMLRLVDRGRLDLDADVNVYLPSWQVPANG
                     DWQPRVTLRQLASHSAGLTVHGFPGYQPGTPLPTTVEILTGTAPANTAGVRVDIVPGV
                     QFRYSGGGTTVMHLVLEEVTGTPLADLIRELVLDPLDMKHSGYEQPLPEMLHDEAATA
                     HRTDGSPVDGRWHVYPELGAAALWTTPTDLCRYAIGVQQALAGATGGLISAELAEAML
                     TPQAPPSPGAERIGRLNSVGLGPFLRHEDGRTTYFGHSGGNEGFRCHLLAHRDGFGAA
                     VMTNSDNGGWLLADVFDAIAQAHGWDGYASDRMGELPPRGTALDRFTGVFELPSGMGV
                     DVVRRGDRLDVTIGSQPPIGFAASTASRLESMFVDATLTLDDAGNLLLRQSDQEHVCR
                     RR"
     gene            1780..2562
                     /locus_tag="VH419_12270"
     CDS             1780..2562
                     /locus_tag="VH419_12270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020577250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding oxidoreductase"
                     /protein_id="HEY7044436.1"
                     /translation="MTSPILVTGGTGTLGRLLVPLLRDAGCSVRVLSRRTHEESGDVE
                     YVVGDLATGTGVQAAVEGTDVIVHCAGNSKGDEDTTRTLVEAASRAGTGHLVYISVVG
                     ADRIPFESRVDRMMFSYFASKRAAEVVVSESGLPWTTLRATQFHDLLMTVARQLAKLP
                     VIPVPAGFRFQPVDTGEVAARLVELALGQPAGMVPDIAGPRVYSTEELIRGYLRACGK
                     RRPLLPIRLPGKAAGAFRAGAHLAPDRAVGRRTWEDFLATRT"
     gene            2781..3803
                     /locus_tag="VH419_12275"
     CDS             2781..3803
                     /locus_tag="VH419_12275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEY7044437.1"
                     /translation="MERDLHWVGWLWLVVVGFAAVALWRSAVLDIPFRDPGGRWLTGK
                     LLATVEAFAVLVVIDAIWRSPRGARRPRNVAHTLRQRWPWRRLLLVASGLLAYHAVYF
                     CYRQLKSWDVFNANQDELLDRADRWLFGGHSPAVLLQDLLGRHDATYVLLVIYESFTW
                     VVLVSFVASVVFVDRLRDGFVCLASLIWVWILGVASYYLIPSLGPFHVRPQDFADLPR
                     TLVTEHQELYMDQRAYLLAHPEAADAYAQVSAFASLHVAVLSVIVLMAWYYGLRRVAV
                     ALAVYLLATMVATVYLGWHFVLDDIAGVLIAVAAVALGRLTIYPRGWQAPPGLPIEAQ
                     SGRVTT"
     gene            3800..4231
                     /locus_tag="VH419_12280"
     CDS             3800..4231
                     /locus_tag="VH419_12280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008359574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEY7044438.1"
                     /translation="MSDSSRSVSLERLSKGRYRATNKHGDSIVVGEGDGAFSPVEMLL
                     TSLAACSAIDVDYIVGKRDEPTSFRLRADANKVRDEGGNHLTDIVVTFDARFADSEAG
                     NAAAEVLPRAVQQSHDRLCTVSRTVELGVAVESVIGQVDSS"
     gene            complement(4274..4888)
                     /locus_tag="VH419_12285"
     CDS             complement(4274..4888)
                     /locus_tag="VH419_12285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012919330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044439.1"
                     /translation="MKLYAASTPHRVRQVFGDVLLLVWVLICIRIGSAVYDATLKLAA
                     PGEGIASAGDSLASHLREAGETVGDVPLVGEQVRPPFDSAGQAADSLANAGQTQVDVV
                     HDLATWLGVAIALIPILIVLAFYVPLRLRFILEATAGQRFIDATEDLDLFALRAMTRQ
                     PMHRLARISDDPAGAWRRRETDVIRRLAELELKDTGLALPRLPA"
     gene            complement(4917..6155)
                     /locus_tag="VH419_12290"
     CDS             complement(4917..6155)
                     /locus_tag="VH419_12290"
                     /inference="COORDINATES: protein motif:HMM:NF018479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HEY7044440.1"
                     /translation="MLEPTDRPDAPERVEWLELFFDLVVVAAVAVFSDALREDTTWAG
                     VGIFLVTYGAVWFAWVSVVMYTDVAAELTRVRTVVVSMLLLAVMAAAAPGHAGHRANA
                     FAGAFVLVRVFAARGSLRTGRLMTSWPLLQSGTFTVPWIVSFWVPVPWKYVLWGAAVV
                     FELGLVMVRGGHLAEQAVSQYQQRMDKLRQHKVRREPSRGSDRELPELQEVAVDREHL
                     DERLGLFVIIVLGESVAALVLIAARSEWTREFVSTAFASFVLLVLLWLLTFSYGFASA
                     PGVRLGDLAPRHGLPIHLLSTTGILLIGVGLGEAAATGERLHGLIVWLTCGGLALHAL
                     ASGIAGLLGRIGRFWLLSCALPTVIFPLVVAVVAAGFEDGLGNPLLIWLLALPVGWQF
                     LYAQRHSALLRLGKLSPSES"
BASE COUNT         1026 a         2159 c         2174 g          998 t
ORIGIN      
        1 tgccggcgct accgcgggtc cgccgccagt cctcttttcc tgatcaacca ctggctcgac
       61 cggccgcaca cactcgtctc ggacgcggcc aaggtgaacg cgtacgacgt cttgtggccg
      121 cgcgtcgaga agtgtcaaca gcaacgtgcc ctgctcccga attacgtcgc ggtgaacttc
      181 tacgacaagg gtgacctgct cgacgtcgtc gaccagctca acgagctcga ctgagcacgc
      241 accaggtcga cggaggcgcc gggtccggct cagccaccaa ccacaacacg gttggacacg
      301 gccggtccca gacggtcgaa tggggcaggt gacttcgaca tcggaagaga ccgacctcta
      361 cggatttccc atcgtcgagc ggatgcggga cttcgacatc cccggcgtca gcattgccgt
      421 catcgaggca gggaccgtcg tcgagaagcg gtgcttcggc gtacgcacca gcactgaccc
      481 ttctcctgtg accgagtcca cccggtttca ggcatgctcg atcagcaagc cggtgtgctc
      541 gctcgccatg ctgcggctcg tcgaccgtgg acggctcgac ctcgacgccg acgtcaacgt
      601 ctacctgccg tcgtggcagg tgccggccaa cggcgactgg cagccacgtg tgacgctgcg
      661 tcagctggcg agccattcgg cgggcctgac cgtgcacggg tttccgggat accagcccgg
      721 gacaccactc cccacgaccg tcgagatcct gaccggtacg gcacccgcga acactgcggg
      781 ggtacgcgtc gacatcgtgc cgggtgtgca gttccggtac tccggcggcg ggacgacggt
      841 gatgcatctc gtgctggaag aggtcacggg caccccgctc gccgacctca tccgcgagct
      901 ggtgctcgac ccgttggaca tgaagcacag tggctacgag cagccgctgc ccgagatgct
      961 gcacgacgaa gcagcgaccg cgcaccgaac ggacgggagt ccggtcgacg gccgatggca
     1021 cgtgtacccc gagctcggag cggccgcgct gtggacgact ccgaccgacc tgtgccgcta
     1081 cgcgatcggg gtgcagcagg cgctcgccgg agcgacgggc gggctgatct cggctgagct
     1141 cgcggaagcg atgttgacgc cccaggctcc gccgtctccg ggcgccgagc gcatcgggcg
     1201 gctgaactcg gtcggactcg gcccgttcct gcgacacgag gacggccgga cgacgtactt
     1261 cgggcacagc ggcggcaacg agggcttccg gtgtcacctg ctggcgcatc gagacggctt
     1321 cggtgccgcg gtgatgacga actcggacaa cggtggctgg ttgctggccg acgtgttcga
     1381 cgcgatcgcg caggcgcacg ggtgggacgg ctacgcgtcc gatcgcatgg gcgagctgcc
     1441 gccgcgtggg acggccctgg accggttcac cggcgtgttc gagctgccgt ccgggatggg
     1501 cgtcgacgtc gtacgccgcg gcgaccggct ggacgtcacg atcggctcgc agccgccgat
     1561 cgggttcgcc gcctcgactg cgagccggct cgagtcgatg ttcgtggacg cgacactgac
     1621 gctcgacgac gccggcaacc tgctgctccg acagagcgac caggaacacg tctgccgtcg
     1681 ccggtaggcg accatcacct gccggaaggc cgggtgccga gtttgctcga gaccgggatg
     1741 cgtgcaggct ggagtggttc cggtgaggag gaagcagcca tgacgtcacc cattctggtc
     1801 accgggggca ccggaacgtt gggtcggttg ctcgtcccgc tgctgcgaga cgcgggctgc
     1861 agcgtccgag tgctcagccg gcgtacacac gaggagtccg gggacgtcga gtacgtcgtc
     1921 ggtgacctcg ccaccgggac aggcgttcag gccgcagtcg agggcaccga cgtcatcgtg
     1981 cactgcgccg ggaacagcaa gggcgacgag gacacgacgc gcacgctggt ggaggcagcg
     2041 tcgcgggccg gcacggggca tcttgtgtac atctcggtcg tgggagcgga caggatcccc
     2101 ttcgagagcc gcgtggaccg gatgatgttc agctacttcg cgtcgaagcg cgcggcggaa
     2161 gtcgtcgtgt cggagtccgg actgccctgg acgacgttgc gggcgacgca gttccacgac
     2221 ctgctgatga cggtggcgcg ccagctggcg aagctgccgg tgataccggt cccggcgggg
     2281 ttccgcttcc agcctgtcga caccggcgag gtggctgccc ggctcgtcga gctcgcgctc
     2341 ggccagcctg ccggcatggt tccggacatc gccggccccc gggtgtactc gacggaggag
     2401 ctcatccgcg gatacctgcg tgcgtgtggg aagcgccgcc cgctcctgcc gatccgactc
     2461 ccgggcaagg cagcaggtgc cttccgggcc ggcgcacacc ttgctcctga ccgggctgtc
     2521 ggccgacgaa cgtgggagga cttcctggcg actcgcacct gaccgtggag tggtcacgcg
     2581 aagcggccag gtagcgtagc catccgctca gtcatcttcg gagcgggcca ggaggaatcc
     2641 ctttggatgt ggatcagagc gagctggtga gccggcgtcc cccacgatct cgccggtcgt
     2701 catggacacc aggccgcggc cgtggacccg atgctcggtt cgccgcaggc ccgcctgcag
     2761 gcaacgacgg ttccgccgac gtggaacgtg acctgcactg ggtcggctgg ctgtggctcg
     2821 tcgtcgtcgg cttcgccgct gtcgctctct ggcggtcagc cgtcctcgac ataccgttcc
     2881 gcgaccctgg cggtaggtgg ctgacgggca agctgctcgc gaccgtcgag gcgttcgccg
     2941 tcctcgtggt gatcgacgcc atctggcgtt cgccgcgtgg cgcaaggcgg cctcgaaacg
     3001 tcgcgcacac gctgcgacag cggtggccgt ggcgccgcct gctcctcgtg gcgagcggcc
     3061 tgctcgcgta ccacgcggtc tacttctgct atcgccagct caagagctgg gacgtcttca
     3121 acgccaacca ggacgagctc ctcgaccgag cggacaggtg gctgttcggc ggccactctc
     3181 ccgccgtgtt gctgcaggat ctgctcggac ggcacgatgc gacgtacgtg ctgctcgtga
     3241 tctacgagtc gttcacctgg gtggtcctcg tctccttcgt ggcctctgtg gtcttcgtcg
     3301 accggttacg cgacggattc gtgtgcctgg cctcactcat ctgggtgtgg atcctggggg
     3361 tggctagcta ctacctcatc ccgtcgttgg gccccttcca cgtcagaccg caggacttcg
     3421 ccgacctccc acgcacgctg gtcaccgagc accaggagct ctacatggat cagcgggcct
     3481 acctgctggc ccatcccgag gccgcggatg cctatgcgca ggtctcggcc ttcgccagcc
     3541 ttcacgtggc cgtgctttcg gtgatcgtcc tgatggcgtg gtactacggg ctgcgccgcg
     3601 tcgcggtcgc gttggcggtc tacctgctgg cgacgatggt ggccacggtc tatctcggtt
     3661 ggcacttcgt gctggacgac atcgccggcg tactcatcgc ggtcgctgcg gtcgcgctgg
     3721 gccggctgac catctacccg cgcggttggc aggctcctcc gggacttccg atcgaggcac
     3781 aatcaggcag ggtgacgaca tgagtgactc gtcccgctcg gtctcgctgg agcggctttc
     3841 caaaggccgc taccgcgcca cgaacaagca cggcgactcg atcgtcgtcg gggagggcga
     3901 cggggccttc agcccggtcg agatgctgct cacctcgctg gcagcgtgca gcgccatcga
     3961 cgtggactac atcgtcggca agcgggacga gccgacgtcg ttccgactcc gtgccgatgc
     4021 gaacaaggtg cgggacgaag gcggcaacca cctcaccgac atcgtcgtca cgttcgacgc
     4081 gaggttcgca gacagcgagg cgggcaacgc ggcagccgag gtgctgccgc gtgccgtgca
     4141 gcagtcccat gatcggctct gcaccgtgag ccgcacagtc gagctcggcg tagcggtcga
     4201 gtccgtgatt ggacaggtgg attcgtcctg agcttatcca cctggccggt ggcgggcggc
     4261 ttagcttgct tgctcacgcg ggcagccggg gcagagcgag accggtgtcc ttcagctcga
     4321 gctcggcgag gcgccggatc acgtcggttt ctcgacgccg ccaggcaccg gcggggtcat
     4381 cgctgatgcg cgcaaggcgg tgcatcggtt ggcgggtcat cgcccgcagc gcgaagaggt
     4441 cgaggtcctc ggtggcgtcg atgaaccgct ggccagcagt cgcctcgagg atgaaccgca
     4501 gccgaagcgg gacgtagaac gccaagacga tgaggatggg gatcagcgcg atcgcaacac
     4561 ccagccatgt cgcgaggtcg tggaccacat cgacctgggt ctggccggcg ttggccaagc
     4621 tgtcggccgc ctgacccgca ctgtcgaagg gcggccgcac ctgttcgccc accagcggca
     4681 catcaccaac ggtctcaccc gcctcgcgca gatgactggc aagcgagtca cccgcgctcg
     4741 ctatcccttc gcctggtgcg gccagcttca gcgtggcgtc gtagacggcc gacccgatcc
     4801 tgatgcagat gaggacccag acaaggagca ggacgtcacc gaacacctga cggacccgat
     4861 gcggagtgga cgcggcgtac agcttcatgg gtccatcctg gcgccgaccg gacgagtcac
     4921 gactccgagg ggctcagttt cccgaggcgc agcagtgcgg agtggcgctg tgcgtagagg
     4981 aactgccagc cgacggggag agccagcagc cagatcagca gtgggttgcc gaggccgtct
     5041 tcgaagcctg cggcgacgac ggcgacgacg agcgggaaga tcacggtcgg cagcgcgcac
     5101 gacagcagcc agaaccttcc gatgcggccg agcagcccgg cgatcccgga ggcgagggca
     5161 tgtagcgcga ggccgccgca ggtcagccac acgatcaggc cgtgcaggcg ctcgccggtg
     5221 gcggccgcct cgccgagccc gacaccgatc agcaaaatcc cggtggtgct gaggagatgg
     5281 atcgggagac cgtggcgcgg cgccaggtct ccgagccgga cgcccggtgc ggacgcgaac
     5341 ccgtaggaga acgtcagcag ccagagcagc accagcagca cgaaggacgc gaacgccgtc
     5401 gagacgaact cccgggtcca ctcgctccgt gcagcgatga gcaccagcgc ggccaccgac
     5461 tcgcccagca cgatgatcac gaacagcccg agccgctcat cgaggtgttc tcggtcgacg
     5521 gccacctcct ggagttccgg gagctcccgg tcgctgcccc ggctcggctc gcggcggacc
     5581 ttgtgctgcc ggagcttgtc catccgctgc tggtactggc tgacggcctg ctcggccagg
     5641 tgtccgccac gcaccatgac cagtccgagc tcgaacacca ccgccgcacc ccagagcacg
     5701 tacttccaag gcaccggcac ccagaacgag acgatccagg ggaccgtgaa cgtcccggac
     5761 tgcaacagtg gccacgacgt catcagccgc ccggtgcgca gtgacccgcg cgcggcgaac
     5821 acccgcacca gcacgaaggc gccggcgaac gcgttggcgc gatgacctgc atgacccggt
     5881 gccgccgccg ccatcacagc aagcagcagc atcgagacca cgaccgtgcg cacccgggtc
     5941 agctcggctg cgacgtcggt gtacatcacg acgctcaccc aggcgaacca caccgcgccg
     6001 taggtcacca ggaagatccc gacgccggcc caggtggtgt cttcacggag ggcgtcggag
     6061 aagactgcca ctgcggccac gacgacgagg tcgaagaaca gctccagcca ctcgacccgc
     6121 tctggtgcgt cggggcggtc ggtcggctcg agcatggacc catcctgcac cgggactcgc
     6181 ggcggagatg ttgcccggct gctctatggt ctagtgcccc gtcaaccaac attcgccctg
     6241 ttgcgcgccg cccgggcggc gatctgcggc gttatcgtcg gtcaacgatg ctgcgcatcg
     6301 cctccctcct ctgccttgca gctcatccac ccggacaccg ctcacgacga gcaacgt
//
LOCUS       DATBFZ010000409         1506 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_1597958, whole genome shotgun sequence.
ACCESSION   DATBFZ010000409 DATBFZ010000000
VERSION     DATBFZ010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1597958"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..508)
                     /locus_tag="VH419_12295"
     CDS             complement(<1..508)
                     /locus_tag="VH419_12295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008356029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEY7044441.1"
                     /translation="MPFLEIEDVHKSYGHHEVLKGVSLDVERHEVVCLIGASGSGKST
                     LLRCVNALEDIQQGAIRIEGDTVTGAGVDLNALRRDVGIVFQSYNLFPHMSVLDNVML
                     APIRVVGISKGEAQERAMELLASVGMQDKAKSYPDMLSGGQQQRVAIVRALAMRPRVM
                     LLDEITAAL"
     gene            complement(512..>1506)
                     /locus_tag="VH419_12300"
     CDS             complement(512..>1506)
                     /locus_tag="VH419_12300"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEY7044442.1"
                     /translation="VAGIAATVVAALRLRDYNDNGVVSTVIGAVLVVLSLYPLYPAFK
                     AFAGDRERSKLLGEDQLVPARRASAAAREEAQIAMGYAAAVLIVAGLLLMAFANDGGV
                     AKTFFTKSAWDQSFNEMIHAFWTNIWAACVAQVLVLIFGLLLAIMRMLPGRAGAPLRM
                     IALVYCDVFRAIPAIVTILLIVFGAPLAFPTTANWNPVWLGIVSLAMTYSAYVAEVYR
                     AGLSSIHPSQSAAARSLGLSYAQTLRTVLVPQAVRRVIPPLLNDFISLQKDTALLSVA
                     AISEVVLVAKLWEAQLFNLSPITLAAAFFIVITIPQARFVDYLIERDARRRAGG"
BASE COUNT          278 a          504 c          503 g          221 t
ORIGIN      
        1 cgagcgcagc ggtgatctcg tcgagcagca tgacccgtgg ccgcatcgcc aacgcgcgca
       61 cgatcgcgac ccgctgctgc tggccgcccg agagcatgtc ggggtaggac ttcgccttgt
      121 cctgcatgcc cacggacgcg aggagctcca tcgcgcgttc ctgtgcttcg cccttcgaga
      181 tgccgacgac ccggatcgga gcgagcatga cgttgtccag caccgacatg tgcgggaaca
      241 ggttgtagct ctggaagacg atcccgacgt cgcggcgaag ggcgttgagg tcgacgcccg
      301 cgcccgtcac ggtgtcgccc tcgatccgga tggcaccctg ctggatgtcc tccagcgcgt
      361 tcacgcagcg cagcagggtg gacttgccgg aacccgacgc gccgatcagg cagaccacct
      421 cgtgccgctc gacgtccagg gagacgcctt tgagcacctc gtggtggccg taggacttgt
      481 ggacgtcttc gatctcgagg aacggcatgg ctcagccccc cgcccgacga cgagcatcac
      541 gctcgatcag gtagtcgacg aaacgtgcct gcgggatcgt gatcacgatg aagaacgcag
      601 ccgcgagggt gatcggcgag aggttgaaca gctgcgcctc ccacagcttt gcgaccagca
      661 cgacctcact gatcgctgcg accgacagca gagcagtgtc cttctgcaga ctgatgaagt
      721 cgttcaacag cggagggatg acccggcgta cggcctgggg aaccaggacc gtgcgcaagg
      781 tctgcgcgta gctgagaccg agcgaccggg cggcggcact ctggctcggg tggatggacg
      841 acagtccggc gcggtacacc tcggccacgt acgccgagta ggtcatcgcc agcgacacga
      901 tgcccagcca caccgggttc cagttcgccg tggtcgggaa cgcgagcggg gcaccgaaca
      961 cgatcagcag gatcgtcacg atcgcgggga tcgcccggaa cacgtcgcag tagaccagcg
     1021 cgatcatccg cagcggggca ccggctcgtc cgggcaacat ccgcatgatc gccaggagca
     1081 gcccgaagat cagcacgagg acctgcgcca cgcaggccgc ccagatgttg gtccagaagg
     1141 cgtggatcat ctcgttgaac gactgatccc acgcgctctt ggtgaagaac gtcttggcaa
     1201 cgccaccgtc gttcgcgaag gccatcagca ggagcccagc cacgatcagc acggcggccg
     1261 cgtatcccat ggcgatctgc gcctcttccc gagccgccgc cgacgcccgc cgtgccggga
     1321 cgagctggtc ctccccgagc agcttggacc gctcgcggtc gcccgcgaac gccttgaagg
     1381 cggggtagag cggatacagg ctcagcacga cgaggacggc accgatgacc gtcgagacga
     1441 cgccgttgtc gttgtagtcg cgcaggcgca gcgcggcgac gaccgtggcg gcgattcccg
     1501 ccacgg
//
LOCUS       DATBFZ010000410        17292 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_1926785, whole genome shotgun sequence.
ACCESSION   DATBFZ010000410 DATBFZ010000000
VERSION     DATBFZ010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 17292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17292
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1926785"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            189..1166
                     /locus_tag="VH419_12305"
     CDS             189..1166
                     /locus_tag="VH419_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007298571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
                     /protein_id="HEY7044443.1"
                     /translation="MSHANACLTAAGRLRLAQLVVDKNWTLRRAAERWNCSVSTAKRW
                     ADRYRAEGRAGMVDRSSRPVTSPRRTPTRVERRVIGLRVSRRWGPARIAYRLKMAVST
                     VHKILHRYGCPPLAWTDPATGVRIKWARRRVTSYVHALPGDLIHVDIKKLGRIPDGGG
                     HRIHGRQQGKKNAGYSRPGYWYIHNAVDDHSRLGYSELLTDERKETAAAFWQRANAYF
                     QSAGIIVKRVMTDNGSCYRSRVFADALGEDIAHKRTRPYRPQTNGKVERFNRTMLEEW
                     AYAKPYHSESERAACFPDWLHTYNHHRGHTALGGKSPADVVPNLRGQNS"
     gene            complement(1539..2285)
                     /locus_tag="VH419_12310"
     CDS             complement(1539..2285)
                     /locus_tag="VH419_12310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013861274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HEY7044444.1"
                     /translation="MRDHDHVSEESLLRPKGARIDDPVSAELGRAAAERRSDVLDGAG
                     MLDLQRTAGNSGVSALLDEDHSPVHDVIASGGHPLDSDTRADMESRLGHDFGDVRVHD
                     DGAAHESAKAVNAHAYTVGSNVVFQRDRYDPSSDAGRLMLAHELTHVVQQRSGPVDGT
                     SAPGGIKVSDPSDRFEREASANAERVMATPASAPTAGPVAQTAAVQRDVDDQAPPAVQ
                     REDAPEEKEDEEEPVQGAFVQREEEEELEE"
     gene            complement(2422..4491)
                     /locus_tag="VH419_12315"
     CDS             complement(2422..4491)
                     /locus_tag="VH419_12315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013861275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY7044445.1"
                     /translation="MSDHGLDHLLARVALVEERVRAVVEQRRAHDPAPDDPFRGLYVN
                     DETVDLLLSGHPPAVAVDDQEARRTEVERQASNDEATGRRIRLRSLARAAPLSGFDVE
                     LLLIAVLPDLDARFERLYGYLNDDVTRRRASVGLALELAGASPMSAQARSHLQQSGPL
                     VDGDLLRVEDPDRPFLTRALRVPDRVTAHLLGDDEPDPLVAPMLVAAVPYPTPLTSEI
                     ARALDHGVRLVHVRERESGTGTALAAAALAEVGLDAVTVDLSWMARDSDPAQLAVVAG
                     REALLRGAGLVAGPVEALAEVSLEALHRLGRWPVPVLLVGTSTWDPRWSTSAPLIVDA
                     PLLGARDRVAMWLEELGNDSDLDPEVLAAHLALGPGQVKNAVRAAQSSALLAGGPMTP
                     ADLRRGVRAQNAAGLERLARRIDPEVGWDDLVLAPPVRSALQELSARARHRDIVLTDW
                     RMRRGGGRGRGVSALFAGDSGTGKTMSAEVIAGDLGLDLYTVNLATVVDKYVGETEKN
                     LERIFTEASGVNAVLFFDEADAIFGKRSDVRDAHDRYANIESAYLLQRLETFDGLAIL
                     ATNLRANIDEAFTRRLDAIIDFPAPTPDLRAALWRQCLTPPLPVSADIDIGFCADAFE
                     LAGGNIRSASITAAYLAAESGRSVGMSDVIRAVEQEYRKLGRLVLEREFGPYFDQLTD
                     QREDRTA"
     gene            complement(4488..5156)
                     /locus_tag="VH419_12320"
     CDS             complement(4488..5156)
                     /locus_tag="VH419_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013861276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4255 domain-containing protein"
                     /protein_id="HEY7044446.1"
                     /translation="MIHEVDSALLALVQREATGTRDVEIVFDAPTKDWAGRRNAPTID
                     IYLYDIREDLRRRERGLLNEYDDQRRIAARWLPPRHFKLSYLVTAWTQRPEDEHRLLS
                     SLLSCFLRYESLPADLLTGPLAELELPVPISVALPPPEDRSFADVWSALGGELKPSLD
                     LVVSAPASTGRRFEAAELVAEPPRVSMGGTGDWPPREQARRQVDREGARTPAPRRRAR
                     KRTG"
     gene            5383..6912
                     /locus_tag="VH419_12325"
     CDS             5383..6912
                     /locus_tag="VH419_12325"
                     /inference="COORDINATES: protein motif:HMM:NF012271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HEY7044447.1"
                     /translation="MTDPILRLDPARVSAEPGGQARVAVTVMNPGTIVEGYDLDVVSQ
                     TPITYAEVVPPFLEVYPGQQGTASVVFSPPSGPQAQGGQIAFGVRARSRVDPTASGVV
                     EGDLEIGRVFGLQAKLTPVTSSGRWRGRHTVAVANWGNAPARLRLVPSDPDQLLGFLI
                     RPEVVEVPLGTTATARLKVRTRKPVLRGTPTRLPFQVVGEPDPPAPVPGPVSPFSDPS
                     RPVVDGAFMQKPILSRVVVAVAAIAALLLIAGTAFALTRPGSTESQEASGAPEPPANI
                     EAIKRGAAFITLAWDAATDVDKYQLITQQNDRNVGTTDVDGALDQFTVADLKPTTDYC
                     FVMIAYRGSLHSGNSQQVCASTAKATSTATPPPPPDGTQTTQTSTGGTESTATTSTTT
                     STTSTTSTTGNNPPPVFTPGQYVNAVRLYPAASSTAEQSANSFKRQLQNNGIDAKVLF
                     TGDYDGLLVGTLHPEPSYMVYVGPYGSFNEALADCGSITPAVPGAECNPAQPVPAPPS
                     S"
     gene            complement(6917..8044)
                     /locus_tag="VH419_12330"
     CDS             complement(6917..8044)
                     /locus_tag="VH419_12330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEY7044448.1"
                     /translation="MRGRRHRGEVMMAGEDVCEACGERNPIDSAFCLYCGVYLGWNER
                     TLDETSAGPEASAPGPAPAGPGHQPAQPQPGGPPPQPEPATTAVQPAVGPGPVRGGAP
                     TAYAPGEAPCPTCGRVNEPGRRFCGKCGQVLQAVPTAAGSGPTPTRRSSWWTRLFDPA
                     DRRARRDYRRSLPAWYRWRRWLITLLVIALVVIGCVIVGKNPVGWAKARWYDLRDTTV
                     AVTPVQASSVPPHSALAGRGAGKLVDGTSNAWVTSWPPKGANASPPCGGLVGVGRARL
                     EFPPTRIRGIEINAGPNADEADSRQLQFVPKRVDVIAGGRCLELADLENVPESQKRSF
                     DTGQPVDQLVLAVATAYAPTAKTGTTPTVQIREVTLLARPD"
     gene            8138..10339
                     /locus_tag="VH419_12335"
     CDS             8138..10339
                     /locus_tag="VH419_12335"
                     /inference="COORDINATES: protein motif:HMM:NF012570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HEY7044449.1"
                     /translation="MTQCSNCDFLNEDGAAFCARCNHYLAWDSETTSENTPTTADQEG
                     RPPATPAPAEPAPTGPPPSAPPPPPPPAPPSAPRRGASPPAARQQPPRVDVAGLISAI
                     DAGRDLAQHRRRPDLVRQLDSTKERLSSRTISVVVVGEFKRGKSTLINALLQMAVCPV
                     DADVVTAVPTRVTYGEQVGVTAYRRTDGGPPRAEPASLERITALVSEHGSNGASPPLT
                     SVDVQVPHRMLRSGLVVVDTPGVGGLESVHGQLTLATLSDADSVLFVTDAGQELTGPE
                     LEFLKTTIQRCASAALVVTKTDIYPEWRRIVDLDIGHLRDAAIQVPVLPVSSFLRLRA
                     ARQPALNEESGFRRLVEHLATVVVVPGTAKAAAEAARDADFVASQLAHEADAERVVLA
                     KPAEEPAVVSQLQRARDKAVKLGAASASWQQTLSDGIQDLVADVEHDLHGRLRGVLRD
                     VEAVIDEGDPKDSWPDIELWLRRQVTVAGMENRDLILSRARELSDSVAKQFDLEAGTG
                     IEVELAGVTQALEQLELAPAASGAMAGGRLGSLVITARISMLVPVILYSVGSILLPGA
                     GLAMAGVGAAMGAGIGGKLFKDESKRQRTTRRQQAKGTARKFVDDVGFVLTKETRDGL
                     RAVQRQLRDDFHNRASVMQRSTAAALEAAQRATQLTPATKAVRVQQVEAETTRLQAVR
                     SSLREFAVGAAPATVVGGAMLGATMVVGGSDGSEIEAPGAPTPDTVIDDHG"
     gene            10332..11864
                     /locus_tag="VH419_12340"
     CDS             10332..11864
                     /locus_tag="VH419_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013861283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HEY7044450.1"
                     /translation="MDELGARVLDVATAARANAVDPDTQQRLDEIAGRLRGPLRLAIA
                     GKVKAGKSTLLNALLGEELAPTDAGECTRILTWYRQSDRPHVIVFPVQGTPVERPYRR
                     DRGALDVDLGPLTHEQVDHLEVGWPTSRLRDLTLLDTPGIASISATVSARTHQVLSAE
                     GDDAVPVADAVLYLLRHTHASDMRFLESFHDDELAHGTPMNAVGVLSRADEIGSARLD
                     AMAVAKRVARRYEADPRMHRLCPLIVPVDGLLGHAAVTLREVEFAAVRTLAEAPQPEL
                     VELLLTADRFARRPTPLPLSTEQRGELLRRLGLFGVRLSVELVRSGARTSAALSEALA
                     QHSGLNELRSVVLRQFESRSRVLKARSAVAALQPLLRGDGVANSAELLARLEQIAANA
                     HEFEEVRMLSELRSGAIEVGGNQAERLDRLLGGSGHDPASRLGLQPDASVDDVRRAAL
                     EQLASWHDVAEHPLSNRSTQLVARTATRTLEGVLAATQPAYPAESGPLNEPLADGVGD
                     SV"
     gene            complement(11823..12449)
                     /locus_tag="VH419_12345"
     CDS             complement(11823..12449)
                     /locus_tag="VH419_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013861285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY7044451.1"
                     /translation="MDTPITTYVHGFDAISRAGVISQLRISPQIRIVDEMDVDQAMVA
                     VVVSDTLDDETARTMRALRRGERPRLVLVATTIDEATLVSAAEIGVGGLIRRVDATHD
                     ALVSTIVRVASGEGDVPGDLLGKLMEQLGRLQRQVLAPRGLTFTGLSPRERDVLRLVA
                     DGLDTAEIAEQMCYSERTVKNVLHDLTSRLQLRNRSHAVAYAVREGLI"
     gene            12587..12865
                     /locus_tag="VH419_12350"
     CDS             12587..12865
                     /locus_tag="VH419_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020387091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3039 domain-containing protein"
                     /protein_id="HEY7044452.1"
                     /translation="MSTRLSPGAETIEDRRTQESLDEGDHDRFSHYARKDKLTEAMVM
                     GTPVVALCGKVWVPSRDPQKYPVCPTCKEIWESLPRGEDPGSGDGADG"
     gene            12873..13775
                     /locus_tag="VH419_12355"
     CDS             12873..13775
                     /locus_tag="VH419_12355"
                     /inference="COORDINATES: protein motif:HMM:NF017393.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease"
                     /protein_id="HEY7044453.1"
                     /translation="MKPLFRTGLVGLALLLGAGAIPFAVPSAASGVHPVVVASGCPSM
                     IPPHDRGVPATITGAIREPRHKTGATDDAKISVRVYVHVLRSRPGTGVSTPRIRRQIA
                     ILNHAYAGAQSRFAAVAPFRFHLVDIDRTTNARWWRMDEGTVAEARAKHALHRGTSRD
                     LNLYIGMNRSGNLGWGTPPAQFKQAPRMDGVVIRRTSMAGGRGGHHSSGDTAVHETGH
                     WLGLLHTFTGRCGARGDLVGDTPREARPSYACPVRRDTCSARGRDPVHNFMDYSYDSC
                     MNQFTAGQVGRMRQQWSAYRRGGG"
     gene            13776..15179
                     /locus_tag="VH419_12360"
     CDS             13776..15179
                     /locus_tag="VH419_12360"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY7044454.1"
                     /translation="MASTSRPATTTVPHRPTSPEFRVLSIGIFALIVGIAFEFMAVAT
                     ALPQAARELGGLGLYPWALTGFLAAAMFANGIAGEICDRIGPRLPMATGATGFAIGLV
                     ISGLSQSMPMLITGRVVQGLGAGFTIVAVYVVIAQCYPESLRPQVMTLISTAWVVPSV
                     IGPFIAGGLTEYISWRWAFLSLVPLLPVAMVGVLPRIGRGAPGEVRRAGRVQLAAVMA
                     VGAGLLQWAGLQAEASHWTTAAVTAVVGIVLVVVAARRLFPPGALRLRRGLPSVIVFR
                     GVTAGGFFGCQAYVPLMMVEHRGASPTMAGLAVAGTALGWSAGAWWQGRPGLQTDRTS
                     LVRRGAFITTIGVVVTSSAAIVTETVTVPAWLAGVGLLVGGLGMGLAMASNAVLLFEF
                     SPVEDRGANSAAIQMSDSLGGLLVIGSAGVMYALGRDSVNQTLLFLSVFGLSAAVMLL
                     SIVVAFRVRPTPTPKRD"
     gene            complement(15314..15979)
                     /locus_tag="VH419_12365"
     CDS             complement(15314..15979)
                     /locus_tag="VH419_12365"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF and ANTAR domain-containing protein"
                     /protein_id="HEY7044455.1"
                     /translation="MATIMYAADDFHEIYRAACSAATFLVSGCDHASLMLMKHGHPVT
                     VAASDDIAREVDRLERKVGNGPCLDAIQEDVVYIDSDITHGTPWPELSALVLDETPVR
                     GMAGFRLLVDDHKEGALNLFSDTAGRLTQASINEAILLVSFVSVALTAASQRQEVTTL
                     RTALATNREIGKAVGLMMAFHKMDDQEAFALLRKTSQDMNIKIAEVAREIVTYHNKRG
                     IGR"
     gene            16190..16645
                     /locus_tag="VH419_12370"
     CDS             16190..16645
                     /locus_tag="VH419_12370"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY7044456.1"
                     /translation="MRPGVLRRANPAEADMIAGWHPIATDEVLDWWRGEDVDPWVMVD
                     ADDRPVAYGELWLDPDEDEVELARLIVAPELRRRGLGQQLTRALMAKAAATGLGLTML
                     RTDPDNEAAIGCYLACGFTPLGPEESAEWNVGQRRDWYWMTLPRPSPRS"
     gene            complement(16734..17210)
                     /locus_tag="VH419_12375"
     CDS             complement(16734..17210)
                     /locus_tag="VH419_12375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016826072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEY7044457.1"
                     /translation="MAIDELDAGILALFAAQPRIGVLEASRRLGVARGTVQARLARLE
                     SSGVVRSWGPELDPVALGYPVMAFVTLEIRQGGGHDPVRDHLALIPEVLEVHTVTGPG
                     DLWCRVVARSNTDLQRVLDSMVAYPGIERSVTMIALADQIRHRVLPLVSSAAAHDS"
BASE COUNT         2657 a         5981 c         5982 g         2672 t
ORIGIN      
        1 ccggaaagcc acctacgcca ccaatcggac acgcgggcgt ggtggtttgc ctcggtagcc
       61 atgcaaacgg acggctagct gtactgacca cggacgttag gtacggcgtg ccggctggac
      121 ggtgacgagg acctccgggt gaggtgtgaa gcgtcgaagc atttccatct cacgaaccgg
      181 aggtcctcgt gtcccacgct aacgcctgcc tgactgcagc cggccgtctg cggctggccc
      241 agctcgtcgt cgacaagaac tggacgctgc gccgtgctgc cgagcgctgg aactgctcgg
      301 tctccaccgc caagcggtgg gccgaccgct atcgggccga gggccgggcc gggatggtcg
      361 accggtcctc acggccggtc acctccccgc gccgaacccc gacccgggtc gagcgccggg
      421 tgatcggact gcgcgtttcg cgacgctggg ggccggcccg gatcgcctac cggctcaaga
      481 tggcggtctc gacggtgcac aagatcctgc accggtacgg ctgcccgccg ctggcctgga
      541 ccgacccggc caccggtgtg cggatcaagt gggccaggcg gagggtcacc agctacgtcc
      601 acgccctacc cggcgacctg atccacgtcg acatcaagaa gctcggccgg atcccggacg
      661 gcggcggcca ccggatccac gggcgccaac agggaaagaa gaacgccggg tacagccgcc
      721 ccggctactg gtacatccac aacgccgtcg acgaccacag ccggctcggc tacagcgagc
      781 tgctcaccga cgaacgcaag gaaaccgctg cggccttctg gcagcgggca aacgcctact
      841 tccagtccgc cgggatcatc gtgaagcgcg tgatgaccga caacggctcc tgctaccgct
      901 cgagggtctt cgccgacgcg ctcggcgagg acatcgcgca caaacggacc cggccttacc
      961 gcccgcagac caacgggaag gtggagcggt tcaaccgcac catgctcgag gagtgggcct
     1021 acgccaagcc ctaccacagc gagtcagaac gcgcggcctg cttccccgac tggctccaca
     1081 cctacaatca ccaccgcggc cacaccgcac tcggcggcaa gtcaccagcc gacgtcgtgc
     1141 ccaacctgcg tggacagaac agctagccag gcagatctgc cttgggagcg atgggtttgc
     1201 cacgggacca atgcagatcg gacccgctga tcggcgaatg tccgagaacc gccggtcaga
     1261 cccccacact gaatggaacg gaacgccggg cgaccggctc agcgggcacg aacggctccg
     1321 ctcttcgttg ccgacccacc ctgagctcat cacgacggaa gccgggcaac caccccaccc
     1381 atgacgacca tctgcggctg gccggcctga tgtctgcgat gtccacatcc gagaccctct
     1441 gtgcccatcg gaggtttgcg atgtgtacac gccgcttggg ctgtaaacat cgcaaacccc
     1501 aaccaccagc ggggcctacg tcccgccgca ggggagggct actcctcgag ctcttcttct
     1561 tcctcgcgct ggacgaaggc gccctgcacg ggctcctcct catcttcctt ctcctccggc
     1621 gcatcctcgc gctgcacggc gggaggggct tggtcgtcga cgtcgcgctg gaccgcggcg
     1681 gtctgcgcga ccgggccggc cgttggcgcg gacgcggggg tcgccatgac gcgctcggca
     1741 ttggccgacg cctcgcgctc gaaccggtcg gacgggtcgc tcaccttgat gccgccaggc
     1801 gccgacgtgc cgtcgacggg cccgctgcgc tgctgcacga catgggtcag ctcgtgcgcg
     1861 agcatgagcc tcccggcatc cgacgaaggg tcgtacctgt cgcgctggaa cacgacgttc
     1921 gagccgaccg tgtaggcatg cgcgttcacc gccttggcgg actcgtgcgc tgcaccgtcg
     1981 tcgtgcactc gaacgtcgcc gaagtcgtgt ccgagccggg actccatgtc ggcacgggtg
     2041 tcggagtcca gcggatgacc gccggatgcg atcacgtcat ggacgggtga gtgatcctcg
     2101 tcgagcagcg cgctgacccc gctgttgccg gcggtgcgtt gcaggtcgag catcccggcg
     2161 ccatccagca cgtcgctgcg tcgctcggcg gcggctcggc cgagctccgc actcaccgga
     2221 tcgtcgatcc gggctccttt cggccggagc aggctctcct cggagacgtg gtcgtggtcg
     2281 cgcatggcga tgtccttccc ggagttgctc gttccaccgt gccacgttcc tcgacggtcc
     2341 ggcagtggtt gcggcgtccg acaagcgcgc actgtgggct gcctgaacgg gcagaccaac
     2401 cgcattccca cacatccgag gtcaggcggt ccggtcctcg cgctggtcgg tcaactggtc
     2461 gaagtacggg ccgaactcgc gttcgaggac gagtcggccg agcttgcgat actcctgctc
     2521 gacggcccgg atcacatccg acatgccgac tgagcggccg gactcggcgg ccaggtacgc
     2581 cgcagtgatc gacgccgagc ggatgttgcc gcctgccagc tcgaaggcgt cggcacaaaa
     2641 gccgatgtcg atgtcggcgg acaccgggag tggcggtgtc aggcactgtc gccacaacgc
     2701 ggcacggagg tcaggggtcg gggcggggaa gtcgatgatc gcgtcgagtc ggcgggtgaa
     2761 ggcctcgtcg atgttggctc gcaggttggt cgccaggatg gccagtccgt cgaaggtctc
     2821 gaggcgctgg aggaggtagg cgctctcgat gttcgcgtag cggtcgtgtg cgtcacgcac
     2881 gtcacttcgt ttaccgaaga tcgcatccgc ctcgtcgaag aacaacacgg cgttcacacc
     2941 gctggcctcg gtgaagatgc gctccaggtt cttctcggtc tcaccgacgt acttgtccac
     3001 gacggtcgcg aggttcaccg tgtagaggtc gagaccgaga tcgcctgcga tcacctcggc
     3061 tgacatcgtc ttcccggttc ccgagtcgcc ggcgaacaac gcgctgacgc ctcggccgcg
     3121 gccgccgccg cgccgcatcc gccagtcggt gaggacgatg tcgcggtgcc tggcacgtgc
     3181 cgacagctct tgcaacgccg atcggaccgg tggagcgagt accaggtcgt cccatccgac
     3241 ctcggggtcg atccggcggg caagacgttc gagtccagcg gcgttctgcg cccggacccc
     3301 ccggcgcagg tcggccggtg tcatgggtcc acccgcgagc agcgctgacg actgggcggc
     3361 tcgtacggcg ttcttgacct ggccgggccc gagcgccagg tgcgcagcga gtacttcggg
     3421 atcgaggtca ctgtcgttcc cgagctcttc cagccacatc gcgacccggt cccgggcgcc
     3481 gagcaacggg gcgtccacga tgagcggagc ggacgtgctc catctcgggt cccacgtcga
     3541 cgtaccgacg agcagcaccg ggaccggcca acggccgagg cggtgcagcg cctccagtga
     3601 cacctccgca agcgcctcga cggggccggc caccagtccg gctccacgca ggagagcctc
     3661 ccgaccggcc acgacggcca gctgcgccgg gtcggagtcc cgggccatcc agctcaggtc
     3721 cacggtcacc gcgtcgagtc ccacctcagc aagcgctgcc gcagccagtg cggtgccggt
     3781 cccgctctcg cgctcgcgga cgtgcaccag gcgcacgccg tggtcgaggg cacgagcgat
     3841 ctcggacgtg aggggcgtcg ggtagggaac cgcggccacc agcatcgggg cgacgagcgg
     3901 gtcgggctcg tcgtctccca acaagtgcgc cgtgacccgg tccgggacac gcaacgccct
     3961 ggtgaggaag gggcgatcgg gatcctcgac gcggaggagg tcgccgtcga cgagcggtcc
     4021 cgactgctga agatggcttc gcgcctgcgc ggacatcggt gacgcgcccg ccagctcgag
     4081 ggccagaccc acgctcgcgc ggcgacgggt cacgtcgtcg ttgaggtatc cgtacagccg
     4141 ttcgaagcgg gcgtcgaggt cgggtaggac tgcgatgagc aggagctcga cgtcgaagcc
     4201 ggacaggggg gcggcgcggg cgagcgaccg cagtcggatc cggcgaccag tcgcctcgtc
     4261 gttgctcgcc tgacgctcca cctcggtccg ccgggcctcc tggtcgtcga ccgcgaccgc
     4321 aggcggatgt cccgacaaca acaggtccac cgtctcgtcg ttcacgtaca gtccgcggaa
     4381 cgggtcgtcc ggcgcggggt cgtgtgcgcg ccgctgctcg accactgcgc gaacgcgctc
     4441 ctcgaccaga gcgacgcggg cgagcaggtg gtcgagcccg tgatcgctca tcccgtgcgc
     4501 ttcctggccc gacggcgagg agccggcgtc ctggcgccct cacgatccac ctgccgtcga
     4561 gcctgctcgc gcggtggcca gtcgccggtg ccgcccatcg acacccgcgg cggctccgcg
     4621 acgagctcgg ccgcctcgaa ccgccgacct gtcgaggcag gtgcgctcac gaccaggtcg
     4681 agcgagggtt tgagctctcc accgagtgcc gaccacacgt cggcgaacga cctgtcctcc
     4741 ggcggcggaa gcgccacgct gatgggcacc ggcagctcga gctcggccaa cggcccggtg
     4801 agcaggtcgg ccggcagtga ctcgtagcgc aggaagcagc tgagcaggct cgacagcagc
     4861 cggtgttcgt cctccggtcg ctgcgtccac gccgtgacga ggtaggagag cttgaaatgt
     4921 cgcggcggca gccaacgggc ggcgatccgc cgctggtcgt cgtactcgtt gaggaggcca
     4981 cgctcacgcc gtcgcaggtc ctcgcggatg tcgtacaggt agatgtcgat cgtcggcgcg
     5041 ttccggcgac ctgcccagtc cttcgtcggt gcgtcgaaga cgatctcgac gtcacgcgtt
     5101 cccgtcgcct cgcgctgaac caacgcgagc agggccgagt cgacctcgtg gatcacctca
     5161 cgagtgtccg gccgagtcgg ctcggactgg caggccgaac cggaccctgc gacggggcag
     5221 ggtctgctgc ccgagtgggc aacagcgagg tgaacgccga ccgctggccg acgccgcgcg
     5281 gaccaggcct gttcgtgacg tgccaacctg catagccttg acgacgcggc ccgtcgggcg
     5341 aaccgggggc cttccgcatg tgttcgaggg aggggtgtga acgtgaccga cccgatcctc
     5401 cggctcgatc ccgcgcgcgt ctccgcagaa ccaggcggac aggcacgcgt cgcggtcacg
     5461 gtgatgaacc ccggaacgat cgtcgaggga tacgacctgg acgtcgtgtc gcagaccccg
     5521 atcacgtacg ccgaggtcgt gccgccgttc ctcgaggtgt acccgggcca gcaggggacg
     5581 gcatcggtgg tcttctcacc acccagcgga ccgcaggcgc agggtgggca gatcgcgttc
     5641 ggtgtccggg ccaggtcacg cgtggacccc acggcgagcg gagtggtcga gggagacctc
     5701 gagatcggcc gcgtcttcgg gctccaggcg aagctcactc cggtgacctc gtcgggccgt
     5761 tggcgtgggc ggcatacggt ggccgtcgcc aactggggaa acgcgccggc tcggttacgg
     5821 ctcgtcccga gtgaccccga tcagctcctc ggcttcctga tacgacccga ggtggtcgag
     5881 gtgccgctgg gcaccaccgc gaccgctcgt ctcaaggtcc gcacccgtaa gccggtgctg
     5941 cgtggcacgc ccacgcgact gccgttccag gtcgtcggcg agccggaccc accggcgccg
     6001 gtaccgggcc ccgtgtcacc gttctccgac ccgagccggc cggtcgtcga cggcgcgttc
     6061 atgcagaagc cgatcctgtc gagagtcgtc gtggcggtcg cggccatcgc agcactgctg
     6121 ctgatcgccg ggacggcgtt tgcgctgacg cgcccggggt cgacggagtc ccaggaggcc
     6181 agtggcgcgc cggaacctcc cgcgaacatc gaggcgatca agcgcggggc cgcgttcatc
     6241 acattggcct gggatgcggc cacggacgtc gacaagtacc agctcatcac gcaacaaaac
     6301 gaccggaacg tcggaaccac cgacgtcgac ggcgcgctcg accaattcac cgttgcggac
     6361 ctcaagccca ccaccgacta ctgcttcgtg atgatcgcgt accgcggatc gttgcactcg
     6421 gggaactcgc aacaggtctg cgcctcgacc gcgaaggcga cgtccacggc gaccccgcca
     6481 cccccaccgg acggaacaca gaccacgcag acgtcgacag gtggcacgga gtccacggcg
     6541 acgacgagca cgaccacttc cacgacgtca acgacgtcga cgaccggcaa caacccgccg
     6601 ccggtgttca ccccagggca gtacgtgaac gccgtcagac tctatccggc ggcgagcagt
     6661 acggcggagc agtccgccaa cagcttcaag cggcagctgc agaacaacgg gatcgacgcg
     6721 aaggtgctct tcaccggcga ctacgacggt ctcctggtgg gaaccctcca cccggaaccg
     6781 tcgtacatgg tctatgtcgg gccgtacggc agcttcaacg aagccctcgc ggactgtggg
     6841 tcgatcacgc cagcggtacc aggcgctgag tgcaatccgg cgcaaccggt gcctgctcca
     6901 ccgtcgtcct gaccgctcag tcgggtcggg cgagcagcgt cacctcccgg atctgcaccg
     6961 tcggtgtggt ccccgtcttg gcggtcggcg cgtacgcggt cgccacggcg aggacgagct
     7021 ggtccaccgg ttgcccggtg tcgaaggacc gcttctgcga ctcgggcacg ttctcgagat
     7081 cggcgagctc gaggcacctg ccaccggcga tgacatcgac ccgcttcggc acgaactgca
     7141 gctgccggct gtcggcttcg tccgcattcg gcccggcgtt gatctcgatc ccgcggatcc
     7201 gggtcggtgg gaactcgagt ctggcgcgcc cgactcccac gagcccgccg cagggaggcg
     7261 atgcgttcgc gcccttcggc ggccagctgg tcacccaggc attcgacgtg ccatcgacga
     7321 gcttccccgc gccgcggcct gcgagcgcgg agtgcggggg cactgacgag gcctgcaccg
     7381 gcgtcaccgc cacggtcgtg tcgcgaaggt cgtaccaccg tgccttggcc cacccgaccg
     7441 ggttctttcc gacgatgacg cagccgatga cgaccagcgc gatgacgagc agcgtgatca
     7501 accaacgtcg ccagcggtac caggccggga ggctgcgccg gtagtcgcga cgggcgcgac
     7561 ggtcggccgg gtcgaacagg cgcgtccacc aagagctgcg gcgggtcggc gtcggtcccg
     7621 agcccgcggc cgtgggaacg gcttggagga cctgtccgca ctttccacag aaccggcggc
     7681 cgggttcgtt gacccgcccg cacgtcgggc acggtgcctc gcccggcgcg tacgccgtcg
     7741 gcgcaccgcc acggaccggc cctgggccta cggcgggctg gaccgcggtc gtggcgggct
     7801 cgggctgcgg gggcgggccg cccggttgcg gttgagcggg ctgatggccc gggccggccg
     7861 gtgccgggcc gggcgcggac gcctccggcc cagcagacgt ctcgtcgagg gtccgctcgt
     7921 tccacccgag atacacgccg cagtacaggc agaacgcgct gtcgatcggg ttgcgttcgc
     7981 cgcacgcctc acagacgtcc tcgccggcca tcatcacctc cccacggtga cgtcgaccac
     8041 gcatgtgctc ggatcgatga tcgacaaagc atgctccacc gccgattatg gctgaaaggc
     8101 ccagcgattc ggcggcggcg aacgctcagg tggtccgatg actcagtgca gcaactgcga
     8161 cttcctcaac gaggacggtg cagcgttctg cgcgcgctgc aaccactacc ttgcctggga
     8221 ctcggagacg acgtccgaga acactcccac caccgccgac caggaaggca gaccacctgc
     8281 gacgcccgct cccgccgagc cggctccgac cggaccaccg ccgtccgcac caccgcctcc
     8341 accacctccg gctccgccgt ctgcaccacg acggggcgct tcgcctcccg cagcgcgaca
     8401 gcaaccgccg cgggtcgacg tcgccggcct gatctcggcg atcgacgccg gccgcgacct
     8461 cgcgcagcac cgaaggcggc ctgacctggt gcgccagctc gactcgacca aggagcggct
     8521 gagctcgcga acgatctctg tcgtcgtcgt aggggagttc aagcgtggca agagcacgtt
     8581 gatcaacgcg ctgctgcaga tggcggtctg cccggtcgac gccgatgtcg tcaccgcggt
     8641 cccgacgcgg gtgacctacg gcgagcaggt cggggtcacg gcgtaccggc ggaccgatgg
     8701 cggtccaccc cgtgcggagc ctgcgtcact cgagcgcatc actgcgctgg tgtccgagca
     8761 cggcagcaac ggtgcttcgc ctcccttgac gtccgtcgac gtccaggtgc cacaccggat
     8821 gctgcgatcc ggcctggtcg ttgtcgacac ccccggggtg ggcgggctgg agtccgtgca
     8881 cgggcagctc acgctggcca ccctgtccga cgccgacagc gtgctgttcg tcaccgatgc
     8941 ggggcaggag ctcaccggac ccgagctcga gttcctgaag acgacgatcc agcggtgtgc
     9001 ctccgccgcg ctggtggtga cgaagaccga catctatccg gagtggcgac gcatcgtgga
     9061 tctcgacatc gggcacctcc gggacgccgc aatccaggtg ccggtgctcc cggtctcgtc
     9121 gttcctcagg ctgcgggcgg cgagacaacc tgccctcaac gaggagtcgg gatttcgccg
     9181 gctcgtcgag catctggcca ctgtcgtcgt ggtgccgggc acggccaagg cggccgccga
     9241 agcggcccgc gacgccgact tcgtggcctc acagctcgcg cacgaggcgg acgcggagcg
     9301 tgtggtgttg gcgaagccgg ccgaggagcc tgctgtcgtc agccagctgc aacgggcgcg
     9361 ggacaaggcc gtcaagctgg gcgcagccag cgcgagctgg cagcagaccc tgagcgacgg
     9421 catccaggac ctggtcgccg atgtcgagca cgacctgcac ggtcgactgc ggggcgtgct
     9481 tcgagacgtg gaggccgtga tcgacgaggg cgatccgaag gactcctggc ccgacatcga
     9541 gctgtggctg cgccggcagg tcaccgttgc gggtatggag aaccgcgacc tgatcctgag
     9601 ccgcgcgcgt gagctcagcg actcggtcgc caagcagttc gacctcgagg caggaaccgg
     9661 gatcgaggtg gagctcgccg gggtcacgca ggcgctggag cagctcgagc tcgcaccggc
     9721 ggccagcgga gcgatggccg gtggccggct cggctccctc gtcatcaccg cgcgaatctc
     9781 gatgctggtg ccggtcatcc tctacagcgt cggctcgatc ctgctgcccg gtgcaggtct
     9841 tgcgatggcg ggtgtcggtg ccgccatggg tgcaggtatc ggcggcaagc tcttcaagga
     9901 cgagagcaag cgccaacgca cgacgcgacg gcagcaggcc aagggcacgg cgcggaagtt
     9961 cgtcgacgac gtcgggttcg tgctcacgaa ggagacgcgg gacgggctgc gggctgtaca
    10021 gcgccagctt cgcgacgact tccacaaccg ggccagcgtg atgcagcggt cgacggcggc
    10081 cgcactggag gccgcccagc gcgccacgca gctgactccc gcgacgaaag ccgtgcgagt
    10141 gcagcaggtg gaggccgaga ccacccgatt gcaggccgtg cgctcgagcc tgcgtgagtt
    10201 cgcggtcggc gccgccccgg ccaccgtcgt cggtggggcg atgctcggcg cgaccatggt
    10261 cgtgggcggg tccgacggaa gcgagatcga ggcgccgggt gctcccacgc cggacaccgt
    10321 cattgacgac catggatgag ctcggcgccc gggtgctgga cgtcgccacc gccgcgcgcg
    10381 ccaacgccgt cgaccctgac acgcagcagc gtctcgacga gatcgccgga cggctgcgtg
    10441 ggccgctacg gctggccatc gcgggcaagg tgaaggccgg caagtcgacg ctgctgaacg
    10501 ccctgctggg agaagagctg gcgccgaccg atgcggggga gtgcacgcgg atcctcacct
    10561 ggtatcggca gtccgaccgc ccccacgtca tcgtgttccc cgtacagggg acccccgtcg
    10621 agcgaccata ccgacgtgac cggggtgcac tcgacgtgga cctcgggccg ctcacccacg
    10681 agcaggtcga ccacctcgag gtcggctggc cgaccagccg gctgcgtgac ctgacgttgc
    10741 tcgacacgcc aggcatcgcg tcgatctcgg caacggtgtc cgcgcgaacg caccaggtct
    10801 tgtcagccga aggcgacgac gccgtcccgg tcgctgacgc ggtgctgtac ctgctgcgcc
    10861 acacgcacgc gagcgacatg cggttcctgg agtcgttcca cgacgacgag ctcgcgcacg
    10921 ggacgccgat gaacgcggtc ggtgtgctct cccgggccga cgagatcggc tcagcacggc
    10981 tggatgcgat ggcggtcgcg aaacgggtgg ctcgccgcta tgaggcggac ccgcggatgc
    11041 accggctgtg tcccttgatc gtcccggtcg acgggctgct cggccatgcg gcggtgacgt
    11101 tgcgcgaggt ggagttcgcc gcggttcgga cgttggcgga agccccgcag cccgagctcg
    11161 tcgagctgct gctcaccgcc gaccggttcg ctcgccgtcc gacgccgttg ccgctcagca
    11221 cggagcagcg aggcgagctg ttacgccggc tggggctgtt cggcgtacgg ctcagcgtcg
    11281 agctggtgcg gtctggcgcc cggacgtcgg cagcgctcag tgaggcgctc gcccagcaca
    11341 gcggtctcaa cgagctgcgc tccgtcgtcc tgcgccagtt cgagtcgcgc agtcgcgtgc
    11401 tcaaggcgcg ttccgccgtc gcggcgttgc agcccctgct tcgcggggac ggagtcgcca
    11461 acagtgctga gctgcttgcg aggctcgagc agatcgccgc gaacgcgcac gagttcgaag
    11521 aggtccggat gctctcggag ctacgttcag gtgcgatcga ggtgggtggc aaccaggccg
    11581 aacgtctcga ccggctgctc ggtggttccg ggcacgatcc cgcctctcgg ctcggcctgc
    11641 agccggacgc gtccgtcgac gatgtacgcc gagccgccct cgagcagctc gcctcatggc
    11701 acgacgtcgc cgagcatccg ctgtcgaacc gctccaccca actggtcgcg cgcaccgcca
    11761 cccgaactct cgagggggtg ctggccgcga cgcaaccggc atacccagcg gagtctggac
    11821 cgctaaatga gcccctcgcg gacggcgtag gcgacagcgt gtgaccggtt ccgtagctgc
    11881 aggcgtgatg tgaggtcgtg caacacgttc ttgaccgtgc gctcgctgta gcacatctgc
    11941 tctgcgatct cggcggtgtc cagcccgtcg gcgacgagcc gcagcacgtc gcgctcgcgt
    12001 ggcgagaggc cggtgaacgt gagtccacgc ggagccagca cctgtcgctg caggcggccg
    12061 agctgttcca tcagcttgcc gaggaggtct ccagggacgt caccttcgcc ggacgcgacc
    12121 cgcacgatcg tgctgaccag cgcgtcgtgg gtggcgtcca cccgccggat gagcccaccg
    12181 acgccgatct cggccgcgga caccagcgtg gcctcgtcga tcgtcgtggc cacgagtacc
    12241 agccgaggcc gttcgccgcg ccgcagcgcg cgcatggtcc gcgcggtctc gtcgtcgagc
    12301 gtgtcggaca cgacgacggc aaccatcgcc tggtccacgt ccatctcgtc gacgatcctg
    12361 atctgtggcg agattcgcag ctggctgatg accccggcac gcgagatggc atcgaagccg
    12421 tgtacatacg tcgtgatcgg tgtgtccatg cgccctcccg tctggcctgg tccggccagt
    12481 ctgcaacggc gcgctcaacg cccgctcaac gtcccctcaa cagcgttgtc agaaaccggt
    12541 agggctatgg ctccatcagc ttctgacaac tcgtagactt gagccggtga gcacccggtt
    12601 gagccctggc gcggagacga tcgaggatcg gcgtacccag gagtccctgg acgagggcga
    12661 ccacgatcgc ttctcccact acgcccgcaa ggacaagctc accgaggcga tggtgatggg
    12721 cacgccggtc gtggctctgt gcgggaaggt gtgggtgcca tcgcgggacc cgcagaagta
    12781 cccggtctgc cctacctgca aggagatctg ggagtcactg cctcggggcg aggaccccgg
    12841 atccggcgac ggcgctgacg gctgacgcca tcgtgaagcc gctcttccgc acaggcctcg
    12901 ttggcctggc tctgcttctc ggcgccggtg caatcccgtt cgccgtaccg tctgccgcct
    12961 ccggcgtgca ccccgtcgtg gtcgcgtcgg gctgcccgtc gatgattccg ccccatgacc
    13021 ggggcgtgcc ggcgacgatc accggcgcga tccgagagcc gcgccacaag accggcgcga
    13081 ccgacgacgc aaagatctcg gtccgcgtgt acgtacacgt gctgcgctcg cgacccggca
    13141 ccggggtcag cacgccgcgc atccggcgtc agatcgcgat cctgaaccac gcgtacgcgg
    13201 gtgcgcagag ccgcttcgca gcggtcgcgc ccttccggtt ccacctcgtg gacatcgacc
    13261 gcaccacgaa cgcgcgttgg tggcggatgg acgaaggcac cgttgccgaa gcacgcgcga
    13321 agcacgccct gcaccgtggg acgtcccgcg acctcaacct ctacatcggc atgaaccggt
    13381 ccggcaacct cggctggggc acaccgccgg cccagttcaa gcaggcaccg cgcatggacg
    13441 gcgtcgtgat acgccggacg agcatggcag gcggccgagg agggcaccac agctcaggcg
    13501 acacggcggt gcacgagacc gggcactggc tcggtctgct gcacacgttc accggccgct
    13561 gtggcgctcg cggcgatctc gtcggggaca caccgcgtga ggcgcggccg tcgtacgcgt
    13621 gtccggtccg cagagacacc tgctccgccc ggggccggga cccggtgcac aacttcatgg
    13681 actactcgta cgactcctgc atgaaccagt tcaccgccgg acaagtcggc cgcatgcggc
    13741 agcagtggtc ggcgtaccga agaggcggcg gctgattggc ttccacttcg cgcccggcga
    13801 cgacgacggt gccgcaccgc ccgacctcgc cggagttccg cgtgctgtcg atcggcatct
    13861 tcgcactcat cgtcgggatc gcgttcgagt tcatggcggt ggcgacggcc ttgccgcaag
    13921 cggcccgtga gctcggcggc ctcgggctgt acccgtgggc gctgaccggc ttcctggccg
    13981 cggccatgtt cgcgaacggc atcgccggtg agatctgcga ccggatcggg ccacggcttc
    14041 cgatggccac cggagccact gggttcgcga tcgggctcgt catctccggg ctgagccagt
    14101 cgatgccgat gctcatcacc ggccgcgttg tgcaggggct gggtgcgggc ttcacgatcg
    14161 tcgcggtgta cgtcgtgatc gcccagtgct accccgagtc gctccggccg caggtgatga
    14221 cgctcatctc gacggcctgg gtggtccctt cggtcatcgg accgttcatc gcaggaggcc
    14281 tgacggagta catctcctgg cggtgggcgt tcctgtccct cgtaccgctg ctgccggtcg
    14341 cgatggtcgg cgtcctgccg cggatcggtc gtggtgcgcc gggtgaggtc cggcgtgccg
    14401 gccgggtcca gctggccgcg gtcatggcgg tgggcgccgg cctgctccag tgggccggcc
    14461 tccaggcgga ggcgagccat tggacgacgg ccgcggtcac cgcggtggtc ggaatcgtcc
    14521 ttgtcgtggt ggcggcgcga cggctgttcc cgccgggcgc gttgcgactg cgtcgcgggc
    14581 tcccgtccgt catcgtcttc cgcggggtga ctgctggcgg cttcttcggc tgccaggcgt
    14641 acgtgccctt gatgatggtc gagcatcgcg gcgcaagccc gacgatggct gggcttgccg
    14701 tcgccggcac cgcgctcggg tggtcggcgg gagcctggtg gcagggccgt cctggcctgc
    14761 agacggatcg cacgagcctg gtgcgacgtg gcgccttcat caccacgatc ggcgtcgtgg
    14821 tgacgtcctc ggcggcgatc gtcacggaaa cggtcaccgt gcctgcgtgg ttggcgggtg
    14881 tcgggctgct cgtcggcgga ctcggcatgg ggctggcgat ggcgagcaac gcggtgctgc
    14941 tcttcgagtt ctcgccggtc gaggaccgcg gcgccaactc ggccgcgatc cagatgagcg
    15001 actcactggg cggtctgctc gtcatcggct ccgctggcgt gatgtacgcg ctcggccgtg
    15061 actcggtgaa ccagacgctg ctcttcctga gcgtcttcgg cctgtcggcc gcagtgatgc
    15121 tgctgtcgat cgtcgtcgcc ttccgcgtcc gaccgacacc gacgccgaag cgtgattgaa
    15181 ccgctaccaa ggtgggcatg gaagggcatg gcgttggtcg gccgcgggcg gttggcgcct
    15241 atcgggtggc gttgagcaca acagcgtggc tcgggggctg gggtggaagc caaccgcgct
    15301 tccacccatc agctcatcga ccgataccgc gcttgttgtg gtacgtcacg atctcgcggg
    15361 cgacctccgc gatcttgatg ttcatgtcct gtgacgtctt gcggagcaat gcgaacgcct
    15421 cttggtcatc catcttgtgg aacgccatca tcaggcccac cgccttgccg atctcccggt
    15481 tggtcgccag cgccgtccgc agtgtggtca cctcctgacg ctgggacgcc gcagtgagtg
    15541 cgaccgatac gaacgacacc agcaggatcg cttcgttgat ggaggcctgg gtcaaccgtc
    15601 cggcggtgtc ggagaacagg ttgagcgctc cctccttgtg gtcgtcgacg agcagccgga
    15661 aaccggccat gccgcggacg ggagtctcgt cgagaaccag ggcgctgagc tctggccacg
    15721 gtgtcccgtg ggtgatgtct gagtcgatgt acaccacgtc ctcctggatc gcgtccaggc
    15781 aaggcccatt gccgactttt cgttcgagcc tgtcgacctc ccgggcgatg tcgtcactcg
    15841 cggcaaccgt gaccggatgg ccatgcttca tcagcatgag gctggcgtgg tcacaaccgc
    15901 tcacgaggaa ggtggcggcg ctgcacgccg cacgatagat ctcgtggaag tcgtcagcgg
    15961 cgtacatgat ggtcgccaac tcgctgaatg tcgtggcggg atctgctgaa gtcccgctgt
    16021 cgtcttgttc agtgtgatcg gtcattgctg gacggctcgg cgcgttccca cgaccgcgcc
    16081 gaggctcctc cctgtgctgg tccaccacgt gaggggcgtg gcgggggcgt cggaaccctg
    16141 cgtactcaag gctcgggccc atcttgccag caaacgtaag tttggcatcg tgagacccgg
    16201 tgtgctgagg cgggcaaacc cggcggaagc ggacatgatc gccggttggc atccgattgc
    16261 gaccgacgag gtgctcgact ggtggcgggg tgaggacgtc gatccgtggg tcatggtcga
    16321 cgcggatgac cggccggtcg cgtacggcga gctgtggctc gaccccgacg aggacgaggt
    16381 cgagctcgcc cggctgatcg tcgcgccgga gctacggcgg aggggactcg ggcagcagct
    16441 cacccgtgct ctgatggcga aggcagcggc gaccggactc gggctgacca tgctgcggac
    16501 cgacccggac aacgaggccg ccatcggctg ttacctggcg tgcgggttca ccccgctcgg
    16561 acctgaggag tcggccgagt ggaacgtcgg gcaacgtcgc gactggtact ggatgaccct
    16621 ccctcgcccc agtcctcgaa gttgaccgcc tcgcaggctc cccgcgaatt gcgggaagtt
    16681 ccgcgatttt ctccctggga atcccggaac ctcccgcaat ccgcggtcaa cggtcaggag
    16741 tcgtgggcgg cggcggagga caccaggggc agtacccggt gccggatctg gtcggccagc
    16801 gcgatcatcg tgacggaccg ctcgatcccg gggtaggcga ccatgctgtc cagcactcgt
    16861 tgcaggtcgg tgttggacct ggccaccacc cggcaccaca ggtccccggg accggtgacg
    16921 gtgtgcacct cgagcacctc cgggatcagc gccagatggt cacgcaccgg gtcgtgtccg
    16981 ccaccttggc ggatctcgag ggtcacgaac gccatgaccg gatagcccag cgccaccggg
    17041 tcgagctcgg ggccccacga ccgcacgacc ccggatgatt caaggcgggc gagccgggcc
    17101 tgtaccgtcc cgcgagccac gccgaggcgg cgggacgcct cgagcacgcc gatgcggggc
    17161 tgcgcggcga acagcgcgag gatcccggcg tcgagctcgt cgatcgccat ccgccacctc
    17221 ccgactggtc atgctgtgca cgatcgcgtc aatgagccca gccgaagtgg gcagattgca
    17281 cagtcgaaca ct
//
LOCUS       DATBFZ010000411         6059 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_4220436, whole genome shotgun sequence.
ACCESSION   DATBFZ010000411 DATBFZ010000000
VERSION     DATBFZ010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 6059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6059
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4220436"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..756)
                     /locus_tag="VH419_12380"
     CDS             complement(<1..756)
                     /locus_tag="VH419_12380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044458.1"
                     /translation="MDGPRRVRRAELLANRMSREQSGVAHRRQLYELGVTRSELRAQL
                     DARRWRSHGRQTIGLHTGPLAGRAMYFRAVFETAGDSALDGVSALIDAGLVGFREDLV
                     HVSVAKSTIHRRPRGVRVHETRRRYDGDAGIVRGLPRVDLAAAAIRAALWAESGRQAS
                     LILIMSVQQQIVTPGALHEAFSRVLRDRRRGLIKAVLSDLADGVRSMGELDFARMCRR
                     RGLPTPSRQQWRRLPDGRACLDVYFDQYGVVVEI"
     gene            complement(962..1714)
                     /locus_tag="VH419_12385"
     CDS             complement(962..1714)
                     /locus_tag="VH419_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HEY7044459.1"
                     /translation="MDRPEPFTPLREDWERALVVVAHPDDMEFGAAAAVARWTGQGKR
                     VIYALVTSGEAGIDGMEPERTRPLREAEQRESCRIVGVDDVEFLGMPDGVLEYGVALR
                     REIAASIRRHRPDIVITNNFRESWDGRVLNQSDHIATGRATVDAVRDAANRWVFPEQV
                     AGGLEPWSGVRQVWVAGSPSSRHAVDITDTFDKGAESLAAHKEYLAGLGWEGFDPMEF
                     LEGIARQTGQRLGTGKATAFEVLALGWEPPED"
     gene            1743..2456
                     /locus_tag="VH419_12390"
     CDS             1743..2456
                     /locus_tag="VH419_12390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012923160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044460.1"
                     /translation="MSSSSSVVARAQTDRGEIVLRHRPVDGALELRVNGVFVMDDHET
                     TTERLLARMALLGLAAGTSDGPWRVLIGGLGLGYTLAALLDRDEVGSVLVAEIEPDLV
                     GWHRSGLIPGSRIADTRVDVRVADVRQVVPGLEPGSVDLILLDVDNGPGFLVYDDNAD
                     VYRAPFLIACRNALTPSGRVAVWSADTAPALTHALDTVFASSEEHAVPVTLGTRDTTY
                     HISIAPRSPLNRELRFVEP"
     gene            2563..2988
                     /gene="arfB"
                     /locus_tag="VH419_12395"
     CDS             2563..2988
                     /gene="arfB"
                     /locus_tag="VH419_12395"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020392918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HEY7044461.1"
                     /translation="MPGPIVVRDGVVVPEHELQWRFSRSSGPGGQSVNTTDSRVELSV
                     DVAASAAFTQTQRQRALDRLRNRLVDGVLTVAASEYRSQLRNRQAAERRMAQILREAI
                     APPPPRRRPTKPTQASVRRRLDSKRQRGELKQQRRAPDA"
     gene            3033..4424
                     /locus_tag="VH419_12400"
     CDS             3033..4424
                     /locus_tag="VH419_12400"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007078398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEY7044462.1"
                     /translation="MSDQEYRIEHDTMGEVKVPATALWRAQTQRAVENFPISGTTLEP
                     AHLHALARIKAAAARVNGELGVVEPAMAEAIEKAAVEVADGQHDDQFPIDVFQTGSGT
                     SSNMNMNEVIASLATRSLGRDVHPNDHVNASQSSNDVFPTSIHVAAAEAITRDLIPAL
                     DHLAKAFESKAEEFATVVKSGRTHLMDATPVTLGQEFGGYAAQVRYGIERLESSLARV
                     AELPLGGTAVGTGINTPPGFPAKVIDVLAELTGLQLTEARNHFEAQGSRDGLVEASGQ
                     LRTIAIGLYKTCNDLRWMGSGPRAGLGEIRLPDLQPGSSIMPGKVNPVLPEAMSMVCA
                     QVVGNDATVAFAGSSGNFELNVMMPVMAANLLQSIRLLANTSRLLADRCVSGIQADVE
                     RCRELAESSPSVVTPLNRFIGYENAAAVAKKAVKERKTIREVVIEEGYVERGDVTEQQ
                     LDEALDVLRMTHP"
     gene            complement(4618..>6059)
                     /locus_tag="VH419_12405"
     CDS             complement(4618..>6059)
                     /locus_tag="VH419_12405"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEY7044463.1"
                     /translation="LAYVHTAANPTASILAGVNTGSQAPPPPAIAGFSSRGPSLAADG
                     DLLKPDIAAPGVDINAATAPEGGIGAGNDFGIISGTSMASPHIAGLAALIIQKHPNWS
                     PMEVKSAMMTTARDLTDTKNPFDEGAGFVEPREFLDPGLVYDSDFSDWADYLSGQGVT
                     VGGRPFTNTPIRASNLNVPSIAINSMAGKESVTRTVTNVGSRTSTYTASVTGLKGLQT
                     TVTPNKLTLAPGQSGTFKVSFSRTTAAFGTYQSGWLFWKDGQHIVRSPIVVNPAGVDA
                     PVEVSPAPNKAFTLTTKSGFTGTMSRRLGGLVASVDTAAEADNSHGAGDPLDPSNYTQ
                     PIQVRGPGTILRVQTLPDNAADDLDLFLLDSDDNVVAQAATGASAEQFTVSGLDKGTY
                     TIAVEAFAVAGGGDSTTFTVRTFSVASATGNLSVTPFVQKVQAAKSSSWAAKTSGLDT
                     SKAYLGVVRWFDGTGTSRIPIGNTLVSIG"
BASE COUNT          977 a         2073 c         2042 g          967 t
ORIGIN      
        1 tatctccacc acaacgccgt actggtcgaa gtagacgtcc aagcatgcgc ggccgtctgg
       61 cagtcggcgc cactgctggc ggctcggcgt cggcagcccg cgccgtcggc acatccgcgc
      121 gaagtcgagt tcacccatcg accggacccc gtcggcaagg tcggacagca ccgccttgat
      181 caggccgcga cgacggtctc tgagcacccg gctgaaggct tcgtgcagcg cgcccggggt
      241 gacgatctgc tgctgcacgg acatgatgag gatgagggag gcctgccgtc cgctctctgc
      301 ccacagcgcc gcccggatcg ctgccgcggc gagatcgacc cgcggaagtc ctcgcacgat
      361 gccggcgtct ccgtcatatc gtcggcgcgt ctcgtgcacg cgcacgccgc gcggtcggcg
      421 atggatggtg ctcttcgcga ccgagacgtg gaccaggtct tcgcggaacc cgacgagtcc
      481 ggcatcgatc agcgcgctca ctccgtcgag agcactgtca ccggccgtct cgaagaccgc
      541 gcgaaagtac atcgcgcgcc cggccagcgg accggtgtgc agcccgatgg tttgtcggcc
      601 atggcttcgc cagcggcgcg catcaagttg ggcgcgaagc tcactgcgcg tcaccccgag
      661 ctcgtacagc tggcggcgat gcgccactcc cgactgttca cgactcatcc tgttggccag
      721 cagctcggcc cggcgtaccc gacgtggtcc gtccatggag gtcagactgg tcagtcaagc
      781 ttccgcgcct cggggcgatc acgccacctg tggacagccg cctcgcgcga ttgcgtccgt
      841 ggaagggctc gcgccggcag ccacctctac ggacgcaacg tggcgagcgc accgttgcgt
      901 ccgtggaagg gattgcggta ggagccgctt ctacggacgc aacgggggcg gaggtgaccg
      961 ctcagtcctc cggaggctcc cacccgaggg ccaggacctc gaacgccgtt gccttgccgg
     1021 tgccgaggcg ttgtcccgtc tggcgcgcga tcccctccag gaactccatc gggtcgaagc
     1081 cctcccaacc gagccccgcg aggtactcct tgtgcgccgc cagtgactcc gcacccttgt
     1141 cgaacgtgtc cgtgatgtcg acggcgtgcc gactcgatgg cgagccggcg acccagacct
     1201 ggcgtactcc ggaccacggc tcgagcccgc ctgccacctg ctccgggaag acccagcggt
     1261 tggccgcgtc tcgaacggca tcgacggtcg ctcgaccggt ggcgatgtgg tccgactggt
     1321 tcaggacccg gccgtcccag ctttcgcgga agttgttggt gatcacgatg tcgggacggt
     1381 gcctccggat cgatgcggcg atctcacgac gcagcgcgac gccgtactcc agcacgccat
     1441 ccggcatacc gagaaactcc acgtcgtcga cgccgacgat ccggcaggac tcccgctgtt
     1501 cggcctcgcg gagcgggcga gtccgctccg gctccatccc gtcgatcccg gcctcgccgc
     1561 tggtgacgag tgcgtagatg acgcgcttgc cctggccggt ccatcgcgcc actgcagcgg
     1621 cagcgccgaa ctccatgtcg tccgggtgcg cgaccaccac cagcgctcgt tcccagtcct
     1681 cccgcagcgg tgtgaacggt tcaggtcggt ccatgtcgtc accgtacggc gagaataggg
     1741 cgatgtcgtc atcgtcgtcc gtcgtggcgc gggcgcagac cgaccgcggt gagatcgtgt
     1801 tgcgccatcg gcccgtcgac ggcgcgttgg agctgcgggt gaacggcgtc ttcgtgatgg
     1861 acgaccacga gaccacgact gaacgcctgc tcgcgcggat ggcgctgctc gggttggcgg
     1921 ccggcacttc tgacggcccc tggcgcgtac tcatcggtgg tctcggactg ggttacacac
     1981 tggcggcgct gctggaccgc gacgaggtcg gctcggtgct ggtggccgag atcgagccag
     2041 acctggtcgg ttggcaccgg tcgggcctga tccccggttc acggatcgcc gacactcgcg
     2101 tcgacgtgcg ggtcgcggac gttcggcagg tggtgcccgg cctggagccg ggatcggtcg
     2161 acctgatcct gctcgacgtc gacaacgggc ctgggttctt ggtgtacgac gacaacgccg
     2221 acgtctaccg cgcgcccttc ctcatcgcgt gccggaacgc tctgacgccg tccggtcgcg
     2281 tggcggtgtg gtcagccgac accgcaccgg cgttgacgca cgccctcgac accgtcttcg
     2341 cttcgtcaga ggagcacgcc gtcccggtga cccttggcac ccgcgacacg acctaccaca
     2401 tctccatcgc tcctcgatcc ccgttgaacc gtgagttgcg gttcgtcgaa ccgtgagatg
     2461 tggttcctcg aaccgtgaga tgtggttgct cgaaccgtga gttctggttc ctcgaaccgt
     2521 gagttgtggt tcctcgaacc gtcagtgcct acgctcgggt gcatgcccgg gccgatcgtc
     2581 gtacgcgacg gtgtcgtcgt acccgagcac gaactgcagt ggcggttctc gcgttcgagc
     2641 ggtccaggtg ggcagtccgt gaacaccacc gacagccgcg tcgagctgag cgtcgatgtg
     2701 gcagcctcgg cggcgttcac gcagacacag cgacaacgcg cgctcgaccg gctgcgcaac
     2761 cgactcgtcg acggagtcct gacggtcgcc gcctcggagt accggtccca gctccgcaac
     2821 cggcaagcgg cggagcgtcg gatggcacag atcctgcgcg aagccatcgc gccgccacca
     2881 ccgaggcggc gaccgaccaa gccgacgcaa gcgtccgtac gccgccggct cgacagcaag
     2941 cggcagcgtg gcgagctcaa gcaacagcgg cgtgcacctg acgcgtgacc gatctcacgg
     3001 cgcacccggg tgcggcgccg tacggtggag ccatgagcga ccaggagtac cggatcgagc
     3061 acgacacgat gggcgaggtc aaggtgccgg ccacggcact gtggcgagca cagactcagc
     3121 gagcggtcga gaacttcccg atctccggta cgacgctcga gcccgctcat ctgcacgcgt
     3181 tggcccggat caaggcggcc gcggcgcggg tgaacggtga gctcggagtg gtcgagccgg
     3241 cgatggcgga agcgatcgag aaggcagcgg tcgaggtcgc ggacggccag cacgacgacc
     3301 agttcccgat cgacgtcttc cagaccgggt cgggcacgtc gagcaacatg aacatgaacg
     3361 aggtgatcgc ctcgctcgcg acccggtcgc tcggacgcga cgtccatccc aacgaccacg
     3421 tcaacgccag ccagtccagc aacgacgtgt tcccgacgtc gatccacgtc gccgccgcgg
     3481 aggcgatcac ccgcgacctg atccccgcct tggaccactt ggcgaaggcg ttcgagtcca
     3541 aggcggagga gttcgcgacg gtcgtcaagt ccgggcgcac ccacttgatg gacgccacac
     3601 cggtgacgct cggccaggag ttcggcgggt atgccgccca ggtccgctac ggcatcgagc
     3661 gcctcgagtc atcgctcgcg cgggtggcgg agctcccgct cggcggtacg gcggtcggta
     3721 ccgggatcaa cacaccaccc ggtttcccgg caaaggtcat cgatgtgctc gcggaactga
     3781 caggtctgca gctgacggag gcgcgcaacc acttcgaggc acaggggagt cgcgacggtc
     3841 tcgtcgaggc cagcgggcag ctgcggacga tcgcgatcgg tctctacaag acctgcaacg
     3901 atctgcgctg gatgggttct ggacctcgtg cgggtctggg cgagatccgg ctgccggacc
     3961 tgcagccggg ctccagcatc atgcccggca aggtcaaccc ggtactgccg gaggccatgt
     4021 cgatggtgtg tgcccaggtg gtcggcaacg acgccaccgt ggcgttcgcc ggctcctccg
     4081 gcaacttcga gctgaacgtc atgatgccgg tcatggcggc caacctgctg cagtcgattc
     4141 gcctcctcgc caacacgtca cggctgctcg ccgaccgctg tgtctcgggc atccaggcgg
     4201 acgtggagcg ctgccgcgag ctcgccgagt cgtcgccgtc ggtggtgacg ccactcaacc
     4261 ggttcatcgg ctacgagaac gccgccgccg ttgccaagaa ggcggtcaag gagcgcaaga
     4321 cgatccgcga ggtcgtgatc gaggaggggt acgtcgagcg gggcgacgtc acggagcagc
     4381 agctcgacga ggccctcgac gtactccgga tgacgcatcc ctgagcggtg acggcggcta
     4441 ccgggcagac ctgatccggc cggtcgacat ggaacggaca tgagaaggcg gcgttccccc
     4501 gtgtgaaagg agaacgccgc ccgtcgcccg acgagcgcga ggcaaaccca cgcctgccaa
     4561 ggcaggtttg cctgggcagc cagccaggtg gatggctgac caggcaaacc accaacctca
     4621 gccgatcgac accagcgtgt tgccgatcgg gatcctgctc gtacccgtgc catcgaacca
     4681 gcgcacgaca ccgaggtacg ccttactggt atccaggccc gaggtcttcg ccgcccacga
     4741 gctggacttc gccgcctgga ccttctgcac gaacggcgtc accgacaggt tgccggttgc
     4801 cgacgccacc gagaacgtgc gcacggtgaa tgtggtcgag tctccaccgc cggccacggc
     4861 gaaggcctca acggcgatcg tgtacgtgcc cttgtccaga ccgctcaccg tgaactgctc
     4921 tgcggacgcg cccgtcgctg cctgcgccac gacgttgtcg tcggagtcga gcaggaacag
     4981 gtcaaggtcg tcggccgcgt tgtccggcag cgtctggacc cgaaggatcg tgcccggccc
     5041 gcggacctgg atcggctgcg tgtagttcga cgggtccaag ggatctccag ccccgtgcga
     5101 gttgtcggcc tcggcggccg tgtcgaccga agcaaccagg ccacccagcc gtcgactcat
     5161 cgtgccggtg aaaccggact tggtcgtcag cgtgaaggcc ttgttgggag cgggcgacac
     5221 ctcgaccggc gcatcgaccc cggccgggtt gacgacgatc gggctacgca cgatgtgctg
     5281 accgtccttc cagaacagcc agccgctctg gtacgtgccg aacgcggccg tggtccggct
     5341 gaacgagacc ttgaacgtcc cgctctgacc cggcgccaac gtgagcttgt tcggcgtgac
     5401 cgtcgtctgc agacccttga ggccagtgac cgacgcggtg tacgtcgatg tgcgcgagcc
     5461 gacgttcgtg accgtgcgcg tcaccgactc cttgcccgcc atcgagttga tcgcgatgga
     5521 cggcacattg aggttcgacg ccctgatcgg ggtgttcgtg aacggacgac cgccgaccgt
     5581 gacgccctgt ccggacaggt agtccgccca gtcgctgaag tcggagtcgt agaccaggcc
     5641 cgggtcgagg aactcacgcg gctccacgaa accggcgccc tcgtcgaacg ggttcttcgt
     5701 gtcggtcaga tcacgcgcgg tcgtcatcat cgccgacttg acctccatgg gagaccagtt
     5761 cgggtgcttc tggatgatga gcgccgccag ccccgcgatg tgcggcgagg ccatcgacgt
     5821 acccgagatg atgccgaagt cgttgccggc tccgattcca ccctcgggcg cagtcgcggc
     5881 gttgatgtcg accccggggg cggcgatgtc cggcttcaac aggtcaccat cggccgccag
     5941 cgacggaccg cggctggaga agccggcgat cgctggcgga ggcggcgcct gggagccggt
     6001 gttcacgccg gccaggatgg aggctgtcgg gttggccgcc gtgtgcacgt acgccagca
//
LOCUS       DATBFZ010000412         4609 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_2909344, whole genome shotgun sequence.
ACCESSION   DATBFZ010000412 DATBFZ010000000
VERSION     DATBFZ010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 4609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4609
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2909344"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="VH419_12410"
     CDS             complement(<1..423)
                     /locus_tag="VH419_12410"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY7044464.1"
                     /translation="MTAQLTLQPETSAAGIARRWVKQQLTELSRPELLEASVLGVSEL
                     VTNALLHVRSTIQVRIVDEAKLRIEVYDHSDRPPEGPPAHMIANGTNPATIGRGLQIV
                     DSISMAWGVAYDGDGKCVWFQPRPEGSIKPSAPSGGAEP"
     gene            complement(548..1243)
                     /locus_tag="VH419_12415"
     CDS             complement(548..1243)
                     /locus_tag="VH419_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008357782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HEY7044465.1"
                     /translation="MSWFAKARDNPSASLLVVQLGAVLVYPFLEGSRTGRSILSVLGI
                     VVLGLAVLTVRRTPSFTWISMLLAAPALVLLAGQIVSDASWLAPWSAAFEAALYFYAA
                     LSLIRYMFADHVVTTDELFAVGATFTLVAWAFAYTYVVVQAVAPDSFTAAVEPGHPRS
                     WVELLFLSFTTLSSTGLSDVVPIRPFARSVVMIEEVAGVLYIAMVVARMVGLTFARIT
                     SRPTERGDGRDSS"
     gene            1327..1677
                     /locus_tag="VH419_12420"
     CDS             1327..1677
                     /locus_tag="VH419_12420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007078881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HEY7044466.1"
                     /translation="MFDVGRAEFEELVADALDELPPDLARLMDNVAIFVEDEAPHGEN
                     LLGLYEGIPLTERTTSYAGAVMPDRVTIFRRNILRICSSAADVRHEVKVTVAHEIGHH
                     FGIDDQRLHDLGYA"
     gene            1724..3388
                     /locus_tag="VH419_12425"
     CDS             1724..3388
                     /locus_tag="VH419_12425"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004035 - alkaline phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HEY7044467.1"
                     /translation="MSSDPLVLGPLLRYVDETSAAIWVETRSAGEVVVQAAGRTWSSR
                     TFAAHGHHYALVSVDELEPGSIQEYAVSVNGVHAWPSPDSAYPPSRIATIKPGKELRL
                     AFGSCRTSVPHDAAGNRAHGVDALRAYALRMAADDHEPWSDLVLFLGDQVYADETSDE
                     MQEFIKARRDINEPPGTELKDFTEYAYLYELAWSEPANRWLLSTIPSAMIFDDHDIRD
                     DWNTSATWRARMKATSWWEDRIVGGLASYWIYQHLGNLSPAERAEDEVWQFLETGWES
                     AAEPDITDMLSKFAARADEFPDSYRWSYARDIGSTRLIVVDSRAARVLQPDDRSILDP
                     KEMSWLQEQLQGGFDHVLIGTSLPFLLSPGLHLFEAWNEAVSSGAWGHRWSGFGEKLR
                     EAVDFEHWAAFQEGFVEVAEGVIEVAEGKRGPAPGTVTFLSGDVHNSYVAQVEPAYRT
                     RIVQAVCSPIRNPLPRSVRYITGFSSKKVGYHIGRLLARSAGVPPGPLTWRTTHGPWF
                     DNSLATLETQGRDLVMRWERGTVDGDAGGDPILEQVAEVRISSTRP"
     gene            complement(4176..>4609)
                     /locus_tag="VH419_12430"
     CDS             complement(4176..>4609)
                     /locus_tag="VH419_12430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007032582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY7044468.1"
                     /translation="KSRSKLDDIVEKALRQAALWDEVKDRLHASAMDLSGGQQQRLCI
                     ARSLATDPEVLLMDEPASALDPKSTARIEDLIFELKRDYTIAIVTHNMQQAARVSETT
                     AFFNLSGVGKPGKLIEYGATSKIFSNPDQEATEAYISGRFG"
BASE COUNT          758 a         1487 c         1606 g          758 t
ORIGIN      
        1 cggctcggcg ccaccagacg gagcggacgg cttgatcgag ccctccggcc gcggctggaa
       61 ccagacacac ttcccgtcgc cgtcgtacgc gaccccccat gccatcgaga tcgagtcgac
      121 gatctgaaga ccacggccga tggtggccgg gttcgtaccg ttggcgatca tgtgggcagg
      181 ggggccttcc ggcggccggt ccgagtggtc gtacacctcg atgcgcagtt tcgcctcgtc
      241 cacgatccgc acctggatgg tgctacgcac atgaaggagg gcgttcgtca cgagctcgga
      301 gaccccgagc acggaagcct cgagcagctc tggccgggat agttcggtga gctgctgctt
      361 gacccatcgc cgcgcgatgc ccgccgcgct ggtttccggc tgcagtgtga gctgcgccgt
      421 cacgtcccct cctcaagcac gacgctccga gttgcgtcgt actggggctc agattacctg
      481 cgcacctcct tgacggcatc acgagacgat ctcgcctacc gggtgaacgg cgtgggcggg
      541 acgccgatca ggaggaatcc cggccgtcgc cgcgttcggt cggacggctg gtgatgcgtg
      601 caaaggtgag gccgaccatg cgcgccacga ccatcgcgat gtagagcacg ccggcgacct
      661 cctcgatcat cacgaccgat cgggcgaacg gccgaatcgg tacgacgtcc gacagcccgg
      721 tgctcgacag cgtcgtgaag ctcaggaaga gcagttcgac ccagctacgt gggtgacccg
      781 gctcgacggc ggcggtgaaa ctgtccgggg ccaccgcctg caccaccacg tacgtgtacg
      841 cgaaggccca cgcgaccaac gtgaacgtcg cccccacggc gaacagctca tcggtggtca
      901 cgacgtggtc ggcgaacatg tagcggatca ggctcagtgc ggcgtagaag tacagggccg
      961 cctcgaaggc cgcgctccac ggcgccagcc aggacgcatc ggagacgatc tgccccgcga
     1021 ggaggaccag cgccggtgcc gcgagcagca tgctgatcca tgtgaacgac ggcgtccggc
     1081 gtacggtcag caccgccagg cccaacacca cgatcccaag cacagacagg atcgagcgtc
     1141 cggtgcggct cccctccagg aaggggtaga cgagcacggc gccgagctgc acgacgagca
     1201 gcgacgccga cgggttgtcc ctcgccttcg cgaaccacga catgagatct cacgctagcg
     1261 tccgagcaag ggcgtccccg ggatcgacag gtccgtccga gccgtgcgcc tgcctatcct
     1321 ggaggcatgt tcgacgtcgg gcgtgcggag ttcgaggaac tggtggccga cgcgctcgac
     1381 gagctccctc cggacctcgc gcggctgatg gacaacgtcg cgatcttcgt cgaggacgag
     1441 gcgccccatg gcgagaacct gctcggcctg tacgagggga tcccgctcac cgaacgcacg
     1501 acgtcgtacg ccggtgccgt catgccggac cgtgtcacga tcttccgtcg caacatcctg
     1561 cggatctgct cgtcagccgc cgacgtgcgg cacgaggtga aggtgacggt ggcgcacgag
     1621 atcgggcacc acttcggcat cgacgaccag cggctgcacg acctcggcta cgcctgacgc
     1681 cgttcgagtg gccgaccttc ccggctcgtt gtagaacgga gccgtgtctt ctgacccgct
     1741 cgtgctggga ccgctgctgc ggtacgtcga cgagacgtcc gcggccatct gggtcgagac
     1801 gcgttccgcc ggcgaggtcg tcgtgcaggc ggctggtcgc acgtggtcat cgcgcacgtt
     1861 cgccgcgcat ggacaccact acgcgctggt ctccgtcgac gaactcgaac cgggatcgat
     1921 ccaggagtac gccgtgtcgg tcaacggcgt acacgcctgg ccgtccccgg actcggcgta
     1981 cccacccagc cggatcgcca ccatcaagcc tggcaaagag ctcaggctgg ctttcggctc
     2041 gtgccggacg agtgtgcccc acgacgccgc cggaaaccga gcacacggcg tggacgcgtt
     2101 gcgggcgtac gccctccgca tggccgctga cgatcacgag ccgtggtcgg acctggtcct
     2161 gttcctgggg gaccaggtgt acgccgacga gacgtcggac gagatgcagg agttcatcaa
     2221 ggctcggcgc gacatcaacg agccacccgg cacggagctg aaggacttca ccgagtacgc
     2281 gtatctgtac gagctggcgt ggagcgagcc ggcgaaccgc tggctcctgt cgacaattcc
     2341 gagcgcgatg atcttcgacg accacgacat ccgcgacgac tggaacacct cggcgacctg
     2401 gcgggcgcgc atgaaagcga cgtcgtggtg ggaggaccgg atcgtcggtg gcctggcgtc
     2461 gtactggatc taccagcacc tcggcaacct gtcacctgcc gagcgggccg aggacgaggt
     2521 gtggcagttc ctcgagacgg gttgggagag cgctgcggag cctgacatca ccgacatgct
     2581 gtcgaagttc gctgctcggg cggacgagtt tcccgacagc taccggtgga gctacgcgcg
     2641 agacatcggc tcgactcggc tgatcgtggt cgactcgcgt gcggcgcggg tgcttcagcc
     2701 ggacgatcgc tcgatcctgg atccgaagga gatgagctgg ctgcaggagc agctgcaagg
     2761 cggcttcgac cacgtgctga tcggcacatc gctgccgttc ctgctgtcac cggggctgca
     2821 tctcttcgag gcctggaacg aggcggtgtc gagcggggcc tggggtcatc gctggtcggg
     2881 tttcggggag aagctgcgtg aggccgtcga cttcgagcac tgggcggcgt tccaggaggg
     2941 gttcgtcgag gtggcggagg gcgtgatcga ggtggcggag ggaaagcgcg gcccggcccc
     3001 tgggacggtc acgttcttgt ccggtgacgt gcacaactcg tacgtcgctc aggtggagcc
     3061 ggcgtaccgg actcggatcg tgcaggcggt ctgctcgccg atccgcaacc cgctgccacg
     3121 ctcggtccgt tacatcaccg ggttctcgtc gaagaaggtc ggctaccaca tcggccggct
     3181 gttggcgcga agtgccggcg tgccacccgg cccgctgacc tggcgtacga cgcacggccc
     3241 gtggttcgac aacagtctgg cgacgttgga gacgcagggg cgggacctgg tgatgcggtg
     3301 ggaacgcgga acggtcgacg gtgacgcggg cggggacccg atcctcgagc aggtcgccga
     3361 ggtccgcatc agctcgacac gtccctgagc gtcaatcgga tccggactcg tcagtgctgg
     3421 tgggcggaga gggtcgcgat ggatggacct gcttccccgc tgcttccgtc gtcatgggcc
     3481 gagggggatc ggcgatgaga gcgggggacc tttcgtcggc gctgagctgg tcggccggct
     3541 ttccatccct tttgggtcgg tgggtgacta gtgggtttcg ggcattcgcc gctggcaggc
     3601 ccggtgtttg cgatgttcag acggcaaacc cactgtgccc atctcaggtt tgcgatgttt
     3661 acacgccttt tgggctgtaa acatcgcaaa ccctgacggc accctggccc gctgaggccg
     3721 gtcggccggc gttccgttgc ttgtggtcgg tatctgactg gcgggtttcg gacatccgcc
     3781 cggtcagcgg cccgatctgc ctcggtcccc acgcagactg accgctccca aggcaggtct
     3841 ccctggctac ccgtccggtt ggatggctac ccaggcaaac caccaccgac gcatgtccga
     3901 atggtggtgc aggtggcttt ccggcgacct gggccgcgcc gatgccgcgg gcaggacgaa
     3961 gtcctgcccg gcaaccctgc ccgaaccggt gcatcgaggc tcacgggtca agggccgccg
     4021 gaggcggtcg cgtcagcgat cgcgagagcg accccttgac gcgtgaggaa tgcaccggta
     4081 gcaggcgcgg cccgcagaag cgtgagaaga accaagatct gcctggctac ccgtccggct
     4141 ggatggctac ccaggcaaac caccaccggg ggaggtcagc cgaagcggcc ggagatgtag
     4201 gcctcggtgg cttcctggtc ggggttggag aagatcttgg acgtggcgcc gtactcgatg
     4261 agcttgcccg gcttgccgac gccggacagg ttgaagaacg ccgtggtctc ggaaacgcgg
     4321 gcggcctgct gcatgttgtg cgtcacgatg gcgatcgtgt agtcgcgctt cagctcgaag
     4381 atcaagtcct cgattcgggc cgtcgatttc gggtccaggg ccgacgccgg ctcgtccatg
     4441 aggagcacct cggggtccgt ggccaacgac cgggcgatgc acagccgctg ctgctggccg
     4501 ccggaaaggt ccatcgcgga ggcgtggagg cggtccttca cctcgtccca cagggcggcc
     4561 tggcgcaggg ccttctcgac gatgtcgtcg agcttgctgc gggacttga
//
LOCUS       DATBFZ010000413         9170 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_3240763, whole genome shotgun sequence.
ACCESSION   DATBFZ010000413 DATBFZ010000000
VERSION     DATBFZ010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 9170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9170
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3240763"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..998
                     /locus_tag="VH419_12435"
     CDS             <1..998
                     /locus_tag="VH419_12435"
                     /inference="COORDINATES: protein motif:HMM:NF014026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HEY7044469.1"
                     /translation="QLGGWSVSWQGVFGAGHVCCMGPPDQIPPGTTVLKGLQATDPNV
                     VSAPDQASAVAAAASANAIVAVVGEKAYAEGLGDNPAPQLPPDQKSLISALEATGKPV
                     IVVVLAGRPVGLGPGENAAGVIMAYQGSTEAGTAVADVVFGSVNPSGKLPVSWPSDAA
                     SVGGDFDGGAPSPLGDQPKFFDQLPGTGSGQGHAYNPLYPFGFGLSYTTFTTTGLTVT
                     PSVSTGGTATATFTLNNTGSRAGTDIVPVFVSQPVSSVVVPPQRLVGFARVTLDPGQS
                     KVVTVAFPVSTLAKSQGDINAAGPPAVERGSYVVQLNKNTTTPYDVAAQASFTIH"
     gene            complement(1109..2482)
                     /locus_tag="VH419_12440"
     CDS             complement(1109..2482)
                     /locus_tag="VH419_12440"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY7044470.1"
                     /translation="MDRHQTDVCIVGAGFAGLAAARRLVEGGARVVVLEARDRVGGRT
                     WNRKMPDGLSVSVGGTWLGVGQDRMFLLAKEVGVDTYEQYSHGDRLLRVDGTTVRYNE
                     SKLPKKYLAGLSSYALAVRRLSRMADRLPSERPWAVADADALDSRTLADWLGSRLNVP
                     SNLGRELTAMAMRVLFCTDPAELSVLGALVLARGGHGSGRGFEYYTDSALTETHLLDG
                     DPPAVADRIAASLGDAVHLSQPVRAITHDDHGVEVHTDDLVVAAGRVIVATPPALAAR
                     IVFEPGLPPTHRHLLMRMVPGAAIRVITTYDEPFWREDGLCGESADPRSPVVITIDQC
                     PRSGSPGVLSSYAFGPPALRIGALPPAERRAAWLAALVDRFGPAAGQPTGYLETDWSA
                     EAWSLGGMISHFSPGTLTTYGTALRAPVGRIHWAGAERATEMHGLIEGAVRSGERAAD
                     EVSRSES"
     gene            2671..2916
                     /locus_tag="VH419_12445"
     CDS             2671..2916
                     /locus_tag="VH419_12445"
                     /inference="COORDINATES: protein motif:HMM:NF015807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HEY7044471.1"
                     /translation="MATLTIRDVDEELRTELRLRAARHGRSMEAEVRAILRSILTKPP
                     SGEGLGTRVHLRFASIGTIDLDVPERLEAPRVPPLPA"
     gene            2913..3335
                     /locus_tag="VH419_12450"
     CDS             2913..3335
                     /locus_tag="VH419_12450"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEY7044472.1"
                     /translation="MIVLDTNVVSELMRAEPSSEVVAWVDRQVADDVFLTAVTLAEVV
                     FGIARLPDGRRKQSLADLVEATVADDFDHRVAAFDAPAAVHYADIAARREHAGRPISA
                     ADALIAAICRSHGAVLATRNVDDFGGVGITVVDPWTYR"
     gene            3427..3987
                     /locus_tag="VH419_12455"
     CDS             3427..3987
                     /locus_tag="VH419_12455"
                     /inference="COORDINATES: protein motif:HMM:NF017090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="emopamil-binding family protein"
                     /protein_id="HEY7044473.1"
                     /translation="MGEVTRQVVPLRRRPLDIAFVAFFAVNLGFVTYIVDLESLVISD
                     PDHFRYPLWPPPPLVDLVHWWGREFDPLLMARPAFWRMTIWIDVLLFGPFYVAAIYAF
                     VKGKEWIRPFAFVWAGMMLSNVLIILLEEADGIYATPRFGIVFLANLAWLVMPILLIA
                     RFLRTPHPFTEPVAAPLVAEPEAVRS"
     gene            3995..4807
                     /locus_tag="VH419_12460"
     CDS             3995..4807
                     /locus_tag="VH419_12460"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY7044474.1"
                     /translation="MGARSSGEFRVRYGPWAIVAGASEGLGRAFATELAGRGVHVVLV
                     ARRAGPLESVAVDIAATGTVEVRTVVADLATTAGHDAVSAAVADLDVGLVVANAAFSP
                     IGSFVETPLDDLVRALDVNCRSALLLARSRVPAMAGRGSGGLVLVSSLAGLQGSPGLS
                     TYAATKAFLTTLAEGLWYELRPLGVDVVACCAGAVGTPGFAGASTRPAPGTLPPEVVA
                     RTTLDALGSGCRVVPGSVNRVAGFLMVRVLPRRAAIAIMAKASSGLARSSPA"
     gene            complement(4840..5703)
                     /locus_tag="VH419_12465"
     CDS             complement(4840..5703)
                     /locus_tag="VH419_12465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044475.1"
                     /translation="MSRRWLLLIPAGLLLAVVVVAVVLLRGSTTTKISVDEAVGQFRD
                     TAVPTSASTISATTTPAATTTTAAPTTAARAITTTTVPIPPVARPETGVYVYTTTGFD
                     SVDALTGARHNFPATTTVTISAAGCGVDVRWNTVRERWDEWQWCPQGRGIETVAYRSF
                     HQFFGVSSGTNYQCAGDPLPLAGRPGSTWTIVCRQGDSDTSRFIGHLIGRQPVKVGTT
                     SVNALRIRYDVAVSGVSAGTKFIDRWIRPEDGLVLREDSGTDTVQSTALGKVHYRERY
                     TIVLQSLVPRR"
     gene            complement(5755..6480)
                     /locus_tag="VH419_12470"
     CDS             complement(5755..6480)
                     /locus_tag="VH419_12470"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEY7044476.1"
                     /translation="MPFADTADDERGALRVLVLQHEEPTPPGLVSDWLDTLGAETDVL
                     RIDLEEPPAEPDRYDLIVSLGSEFGAYDDSLEFVRRETRFLDAAIEADVAILGLCFGG
                     QMLARVLGGDVRRAARAEIGWIQVHTHDPELVPEGPWFQWHFDVMTAPPGATVLAESP
                     AGLQAFVSGRNLGLQFHPEVTPGIVDDWVSDFRYKLAGEGVDPDALLEQTRQRAPENR
                     RATFQLFDAYVERVAALASWSST"
     gene            6679..6963
                     /locus_tag="VH419_12475"
     CDS             6679..6963
                     /locus_tag="VH419_12475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044477.1"
                     /translation="MVNVEGSVTAGVMPLVLCQSEGGPYEDDAFESGWRLGDLAANLA
                     QPGISALADSIRREERHQADLIAMAHGYVMSVEPGDDPVWLSVTFTRSAM"
     gene            7073..7774
                     /locus_tag="VH419_12480"
     CDS             7073..7774
                     /locus_tag="VH419_12480"
                     /inference="COORDINATES: protein motif:HMM:NF039623.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5995 family protein"
                     /protein_id="HEY7044478.1"
                     /translation="MAVLPPAGVPVSSIDEAVARMEEIDAALPPADGLRCFNHMYLGV
                     TLNVGEQVTAGFFDDPEFVSHLDVVFANIYLDAVNAVAESDPVPKAWWPLVESRTMTG
                     IEPIQFALAGMNAHINHDLPLAVNETCVDLGTSPSSGSHHADYQKVDELLADAERQIR
                     QSFEAGVVLEADRHAQAVLDLVCSWSMSSARDVAWNTSMALWSLRRSTLARGLLTNAL
                     ARTTAMASRMLLVDV"
     gene            complement(7894..8340)
                     /locus_tag="VH419_12485"
     CDS             complement(7894..8340)
                     /locus_tag="VH419_12485"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY7044479.1"
                     /translation="MRVAVNGTHVYFDVEGAGLVPDGPMMRERPSLVLLHGGPGFDHS
                     LFKPAYGQLADIAQVVYLDHRGNGRSDRGEPGDWTLDVWADDVRDFCDALGIEHPFVL
                     GWSFGGFVAMHYAARHPDHPAKLILRSTMARWDTDRAVTGFTAVSS"
     gene            8477..8911
                     /locus_tag="VH419_12490"
     CDS             8477..8911
                     /locus_tag="VH419_12490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008356809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY7044480.1"
                     /translation="MAAVPPARYLLRAKDLADARYFEPLTVADLARSARLSPAHFSRE
                     FRQTFGESPHQYLLTRRLERAATLLRTTDWSVARICFAVGLQSVGSFTTSFRRMFGVP
                     PTQYRARYPSAAAHAMVPGCMVRIYGRPANRTFREDGPERLP"
     gene            8948..>9170
                     /locus_tag="VH419_12495"
     CDS             8948..>9170
                     /locus_tag="VH419_12495"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY7044481.1"
                     /translation="MIKIATAQLWVHDQEEALEFYTKKVGMEVRADVTLPEMGDFRWL
                     AVGPGDQPDVSIVLMAIPGPPVMDEETQRQ"
BASE COUNT         1357 a         3124 c         3212 g         1477 t
ORIGIN      
        1 accagctcgg cggctggagc gtcagctggc agggagtgtt cggtgcgggg cacgtctgct
       61 gcatgggacc gcccgatcag atcccacctg ggaccacggt gctcaaggga ctgcaggcaa
      121 ccgatccgaa cgtcgtctcc gcgccggacc aggccagtgc ggtggccgcg gcagcgtcgg
      181 cgaacgcgat cgtggccgtc gtcggcgaga aggcctacgc ggaaggactc ggggacaacc
      241 cggcgccgca gctcccgcca gatcagaagt cgctgatctc cgcgctcgag gcgaccggta
      301 agccggtgat cgtcgtcgtg ctcgctggtc gtccggtagg tctcggcccg ggcgagaacg
      361 ccgctggcgt gatcatggcg taccagggca gcaccgaagc gggcacggcg gttgccgacg
      421 tcgtcttcgg cagcgtcaac cccagcggca agctgccggt cagttggccg tccgacgcgg
      481 caagcgtcgg tggcgacttc gacggcggcg caccctctcc gctcggcgat cagccgaagt
      541 tcttcgacca gctgcccggc accggttccg ggcagggaca tgcctacaac ccgctctatc
      601 cgttcgggtt cggcctctcc tacacgacgt tcacgacgac cggcctcacc gtcacgccga
      661 gcgtgtcgac gggagggact gcaacggcga cgttcacgct gaacaacacc gggagtcgtg
      721 ccggcaccga catcgttccg gtcttcgtga gccaacccgt gagcagtgtg gtcgtgccgc
      781 cgcagcggct ggtcggcttc gctcgcgtga cgctggatcc cggccagtcg aaggtggtga
      841 ctgtcgcgtt cccagtctca acgctggcca agagccaggg cgacatcaat gcggccggac
      901 cgcccgcggt ggaacgcggc tcctacgtcg tccaactgaa caagaacacc acgacgcctt
      961 acgacgtggc cgcgcaggcg tcgttcacga tccactgatc cacgacggcg ggatcgccgc
     1021 gccagctcgg cgcggcgatc cccacgtcac gcgctcggtc gtgtggcatc ggctggtgcg
     1081 tcggtgcggc ctcgatgacg ccgcgcgatc agctttcgga gcgcgacacc tcgtctgcgg
     1141 cccgctcgcc ggaacgtaca gcaccttcga tgaggccgtg catctcggtc gcacgttccg
     1201 cacccgccca gtggatccga ccgacgggtg cccgcagcgc ggttccgtac gtggtcagcg
     1261 tgcccggcga gaagtgggag atcatcccgc cgagcgacca cgcttcggcg gaccagtcgg
     1321 tctcgagata gccggtcggc tggcccgcgg ccggtccgaa ccgatcgacc aacgcggcca
     1381 gccacgcggc gcgccgttcg gcggggggca aggctccgat gcgaagggcg gggggcccga
     1441 acgcgtagct gctgagcacg cccggactcc cgctcctcgg acactggtcg atggtgatca
     1501 cgacgggcga gcgtggatcc gccgactccc cacacagccc gtcctcacgc cagaatggct
     1561 cgtcgtaggt ggtgatgacg cgaatggccg cgcccgggac catgcgcatc aagagatggc
     1621 ggtgggttgg cggcaagccg ggctcgaaga cgatgcgagc ggcgagcgcg ggcggcgtgg
     1681 cgacgatgac gcgcccggcc gccacgacca gatcgtcggt gtgtacctcg acgccgtgat
     1741 cgtcatgggt gattgcgcgc accggctggc tcaggtggac cgcgtcaccg agggaggcgg
     1801 cgatgcgatc ggccacggcc ggcgggtcgc cgtcgagcag atgcgtctcc gtcagcgcgc
     1861 tgtcggtgta gtactcgaag ccgcgcccgc tcccatgccc gccacgggcg agcacgagtg
     1921 cgccgagcac ggacagctcc gccgggtcgg tgcagaacag cacccgcatc gccatggcgg
     1981 tcagctcgcg gcccaggttc gatggcacgt tcagccgcga tccgagccag tcggcgagcg
     2041 tgcgcgagtc gagcgcgtcc gcgtcagcga cggcccacgg gcgctccgaa ggcagacgat
     2101 cggccatccg gctgagccgg cgaaccgcca gcgcgtagct gctgaggccg gcgagatact
     2161 tctttggcag cttcgactcg ttgtagcgca cggtggtgcc atcgacccgg agcaggcgat
     2221 cgccgtggct gtactgctcg tacgtgtcga cgccgacctc tttggcgagc aggaacatgc
     2281 gatcctggcc gaccccgagc cacgtgccgc cgacggatac cgacagtccg tcgggcatct
     2341 tgcgattcca cgtgcgtccg ccgactcgat cgcgcgcttc gagcacgacc acccgggcac
     2401 caccctcgac gagccggcgc gctgccgcga ggccggcgaa gcccgctccg acaatgcaca
     2461 cgtcggtctg gtgccgatcc atgctcagct cctgtcgggt cgatccggcg gtggctgcgg
     2521 tggtggatcg aacattgtcg agccactgag atctcagccc gaggttacga gatcgcttcg
     2581 cagccacggt tcgcctttga ggtcgacgac caccagttct gctagcagtg ctagcatcgc
     2641 tgtcagaccg tctggttcca ggaggccggc gtggcgactc tcaccatccg cgatgtcgat
     2701 gaggagctcc gaacggagct ccgcttgcgc gccgctcgac atgggcgctc gatggaagcc
     2761 gaggtgcggg ccatcttgcg cagcattctg acgaagccgc ccagcggcga ggggctcggc
     2821 acccgggtgc acctgcggtt cgcgtcgatc ggaacgatcg atctcgacgt gccggagcgg
     2881 ctggaggcgc cgcgcgtccc gccgttgccg gcgtgatcgt cctcgacacc aacgtcgtct
     2941 cggagttgat gcgcgccgaa ccgtcgtctg aggtcgtcgc gtgggtcgac cggcaagtcg
     3001 ccgacgacgt gttcttgacc gcggtcaccc tggctgaggt ggtcttcggc atcgctcgcc
     3061 tgcccgatgg aaggcgcaag cagtcgctgg ccgacctcgt cgaggccacg gtcgcggacg
     3121 acttcgacca ccgggtcgcg gccttcgatg cccccgcggc cgtgcactac gcggacatcg
     3181 ccgctcgccg cgagcacgca ggccggccga tcagcgcggc cgacgcgctg atcgcggcga
     3241 tctgccgcag ccacggcgcc gtccttgcga cgcggaacgt cgacgacttc gggggtgtcg
     3301 gcatcaccgt cgttgacccg tggacctacc gctgacccgc accgagcggg tgcggtgctc
     3361 ggttggtcgg cacgtcgccg ctcatcgagc tggcagtacc ctcccgcggt cggggaaggg
     3421 gggacgatgg gcgaggtgac acggcaggtg gtgccgttgc ggcgacggcc gctggacatc
     3481 gccttcgtcg ccttcttcgc ggtcaacctc gggttcgtca cctacatcgt cgacctcgag
     3541 tcgctggtca tctccgaccc ggaccacttc cgctatccgc tgtggccacc gccaccgctg
     3601 gtcgacctgg tgcactggtg gggacgtgag ttcgaccctc tgctcatggc ccggccggcc
     3661 ttctggcgga tgacgatctg gatcgacgtg ctcctgttcg gcccgttcta cgtggccgcg
     3721 atctatgcct tcgtcaaggg caaggagtgg atccggccgt tcgcattcgt gtgggcgggg
     3781 atgatgctct cgaacgtcct gatcatcctg ctcgaggagg cggacggcat ctacgccacg
     3841 ccgcgcttcg ggatcgtgtt cctggccaac ctggcctggc tcgtcatgcc gatcctgctc
     3901 atcgcgcgct tcctgcggac gccacacccg ttcacggaac cggtcgccgc gccgctggtc
     3961 gcggagcctg aagccgtccg gagctgaacg gagcgtgggt gctcggtcgt caggtgagtt
     4021 ccgggtccga tacggaccct gggcgatcgt tgccggagcg tcggaaggcc tcggccgggc
     4081 gttcgccacc gagctcgccg ggcgaggcgt gcacgtcgtg ctggtggctc ggcgggcggg
     4141 accgctcgag tcagtggccg tggacatcgc cgccactgga acggtggagg tgcggacggt
     4201 cgtcgccgat ctcgccacga cagccggcca cgacgcggtg tcggcggcgg tcgccgacct
     4261 tgacgtcggc ctcgtcgtcg cgaatgcggc gttctcgccg atcggatcgt ttgtcgagac
     4321 gccgctcgac gatctggtcc gggcgctgga cgtgaactgc cgatcggcgc tcctgcttgc
     4381 tcgctcgcgg gttccggcga tggcggggcg tggctcgggc ggcctcgtgt tggtctcctc
     4441 gctcgccggt ctccagggat cgccgggcct cagcacgtac gcggcgacga aggcgttcct
     4501 cacgacgttg gcagagggtc tgtggtacga gctgcgcccg ctcggtgtcg acgtcgttgc
     4561 gtgttgcgcg ggcgccgtcg gcacgcctgg gttcgctggt gcctcgacgc ggccggcgcc
     4621 cggcacgctg ccgcccgagg tcgtcgctcg taccacgctc gacgcgctcg gtagcggttg
     4681 ccgagtggtg cccgggagcg tcaaccgtgt ggctgggttc ctcatggttc gtgttctgcc
     4741 ccggcgagcg gcgatcgcca tcatggccaa ggcgtcgagc ggtctggccc ggtccagtcc
     4801 ggcttgacac tgctcaggat cgtctcgatc ggagggcgat cagcggcggg gcacgagcga
     4861 ttgcagaacg atcgtgtagc gctcccggta gtgcaccttg ccgagtgcag tcgactgcac
     4921 agtgtcggta ccgctgtcct cgcgcagcac gaggccatcc tctgggcgga tccaacggtc
     4981 gatgaacttc gtccctgccg acacgccgct caccgcgacg tcgtagcgga tgcggagcgc
     5041 gttcaccgat gtggtcccga ccttgaccgg ctgccggccg atgagatgcc cgatgaaccg
     5101 gctcgtgtcc gagtcgccct ggcggcacac gatcgtccag gtgctcccgg gacggccggc
     5161 gagtggcagc gggtcacccg cacactggta gttggttccc gatgagacgc cgaagaactg
     5221 gtggaagctc cggtaggcga ccgtctcgat cccgcgaccc tgcggacacc actgccactc
     5281 gtcccagcgt tcgcggacgg tgttccagcg cacgtcgacg ccgcagccgg cggcgctgat
     5341 cgtcaccgtt gttgtcgccg ggaagttgtg gcgggctccc gtgagcgcgt cgaccgagtc
     5401 gaacccggtg gtcgtgtaga cgtagacgcc ggtctccggc cgggcgaccg gggggatcgg
     5461 cacggtcgtt gtcgtgatgg cgcgggctgc ggtcgttgga gcggccgtgg tagtcgtcgc
     5521 cgcgggagtc gttgtcgcgc tgatcgttga cgcgctggtc gggaccgcgg tgtcccggaa
     5581 ctggccgacc gcctcgtcga ccgagatctt ggtcgtcgtg ctgccgcgca acagcacgac
     5641 cgcgacgacg accacggcca gcaggagtcc ggccgggatg aggagaagcc agcggcgcga
     5701 catcgagccg tcaggccgca ggcgcgtggc cccgacctgg gcagttcgcg cccgtcacgt
     5761 cgagctccac gacgccagcg ccgcgacccg ctcgacgtac gcgtcgaaca gctggaacgt
     5821 ggcccgacgg ttctctgggg cccgctgcct cgtctgctcg agcagcgcgt ccgggtcaac
     5881 cccctcgccc gccagtttgt agcggaagtc gctgacccag tcgtcgacga tgcccggcgt
     5941 gacctcggga tggaactgga gaccgaggtt gcgcccggac acgaacgcct gcagcccggc
     6001 cgggctctcc gcgagcaccg ttgcgcccgg cggggccgtc atgacgtcga agtgccactg
     6061 gaaccagggc ccttcgggca cgagctcggg atcgtgggtg tgcacttgga tccagccgat
     6121 ctccgccctg gcggcccgac ggacgtcgcc accgaggacc ctcgcgagca tctgaccgcc
     6181 gaagcacagg ccgaggatgg cgacgtcggc ctcgatcgcc gcgtccagga accgagtctc
     6241 gcgccggacg aactcgagcg agtcgtcgta ggcaccgaac tcggagccga gcgagacgat
     6301 gaggtcgtac cggtccggct cggcgggcgg ctcttcgagg tcgatgcgca ggacatccgt
     6361 ctctgcgccg agcgtgtcca accagtcgga caccaggccg ggcggcgtcg gctcctcgtg
     6421 ctggaggacg aggacccgca gcgcgccgcg ctcgtcatcg gcggtgtcgg cgaacggcat
     6481 ggtcctcccc gagcccagtg aacagcacgg cattctcggt ccgtcgcaca cgggttgtcc
     6541 acgtcgtcgc cgcttcgcgc tcgcgcgtca cgtatctccg attccgcaga cgccctcgtt
     6601 acgggaagag ggcccgttct cgatgggctc gtcatcgatc ggtgatgtca gctgccgctc
     6661 gagttggaga ggtgcacgat ggtgaacgtg gagggttcgg tgacagcggg tgtgatgccg
     6721 ctggtgctgt gccagagcga aggcggtccg tacgaggacg acgcgttcga gagcggatgg
     6781 cgactcggcg acctcgcggc aaacctcgcc cagcccggca tcagcgcgct cgccgattcc
     6841 attcgtcgcg aggagcgtca tcaggccgac ctcatcgcga tggcccacgg ttacgtgatg
     6901 tcggtcgagc cgggcgacga tccggtctgg ctgtcggtca cgttcacccg gtcggccatg
     6961 taggtcctcg cgcccgagct cgaccagcgg cgagcaccgt cacaacggtg tggtcggcgt
     7021 ccgcccgacg ccacacagtg ggcgcgggtc gttgcagtcc ctacgatgcg ccatggccgt
     7081 tctcccgccc gcgggcgtac cagtcagctc gatcgacgag gccgtggcgc ggatggagga
     7141 gatcgacgct gccctgccgc ccgcagacgg tcttcggtgc ttcaaccaca tgtacctcgg
     7201 cgtcacgctc aacgtgggcg agcaggtgac ggccggcttc ttcgacgacc cggagttcgt
     7261 ctcgcatctc gacgtggtgt tcgccaacat ctacctcgac gctgtgaatg ccgtcgccga
     7321 atccgaccct gtgccgaaag cgtggtggcc gctcgtcgag tcccgaacga tgacggggat
     7381 cgagccgatc cagttcgcgc tcgccggcat gaacgcccac atcaaccacg acctgccgct
     7441 cgcagtgaac gagacctgcg tcgacctcgg gacgtcgccg agcagcggca gccaccacgc
     7501 cgactaccag aaggtcgacg agctgctcgc cgacgcggag cgtcagattc gccagtcgtt
     7561 cgaagccggc gtcgtcctcg aggccgaccg ccatgcgcag gccgtgctcg atctggtgtg
     7621 cagctggagc atgagcagtg cgcgtgacgt ggcgtggaac acgtcgatgg cgctgtggtc
     7681 gctgcggcgc agcacgctgg cccgcggcct gctcaccaac gccttggcgc gaacgaccgc
     7741 gatggcgagc cgcatgctgc tcgtcgacgt ctgacgcctc gtcgaggcct cacgagtggc
     7801 gactcggacg aagtccagtg gaaataggcg aaaatcggcc ctcagagggt gcagttcggc
     7861 cgagtgagac cgtggtcggg agacgcgctc gactcagctg ctgacggctg taaagccagt
     7921 gaccgcgcga tcggtgtccc agcgcgccat ggtggaccgc aggatcagct tcgccgggtg
     7981 atcgggatgc cgggccgcgt agtgcatcgc gacgaagccg ccgaaggacc acccgagcac
     8041 gaacgggtgc tcgatcccga gtgcgtcgca gaagtcgcgc acgtcgtcgg cccacacatc
     8101 gagggtccag tcgccgggtt cgccccgatc actgcgaccg ttgccgcgat ggtccaggta
     8161 gacgacctgc gcgatgtcgg cgagctgccc gtacgccggc ttgaacagcg aatggtcgaa
     8221 accgggtccg ccgtggagga gcacgagcga cgggcgctca cgcatcatcg ggccgtcggg
     8281 gaccaggccg gccccctcga cgtcgaagta cacgtgggtg ccattcaccg cgacgcgcac
     8341 gtccggcgac gataacccgc cgtttcgtgg gtgaggcgga cgacctcgcg cgccggtcgc
     8401 cgtgtgccgc acggtcggga acgacgtctc tgcaaacgct cgtgtgcgcg ccggcccctc
     8461 tgcgacactg gctgggatgg cagccgtgcc tcccgcccgc tacctgttgc gcgccaagga
     8521 cctcgccgac gcccgctact tcgagccctt gaccgtcgcc gacctggctc gatcggcccg
     8581 gctgtcgccg gcgcacttca gccgggagtt ccgccagacc ttcggggagt caccgcacca
     8641 gtacctcctg acccgccggc tggagcgggc ggcgaccctg ctgcggacca ctgattggtc
     8701 cgtggcgcgc atctgcttcg cggtcgggct ccagagcgtt ggatcgttca cgaccagctt
     8761 ccgacggatg ttcggcgtgc cgccgacgca gtaccgagcg cggtatccgt ctgccgcggc
     8821 ccacgccatg gtgccggggt gcatggtccg gatctacgga cgtcccgcga accgcacgtt
     8881 tcgagaagac ggccccgagc gacttccgta acgtcgtcgt cgaccgttcg atgcaaagga
     8941 gacgacgatg atcaagatcg cgaccgcgca gctctgggtg cacgaccagg aggaggcgct
     9001 ggagttctac acgaagaagg tcggcatgga ggtccgcgcc gacgtgacgt tgccggagat
     9061 gggcgacttc aggtggctgg ccgtcggtcc tggcgaccaa cccgacgtct cgatcgtcct
     9121 gatggcgatc cccggcccgc cggtgatgga cgaggagacc cagcggcagg
//
LOCUS       DATBFZ010000414         8845 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_2258603, whole genome shotgun sequence.
ACCESSION   DATBFZ010000414 DATBFZ010000000
VERSION     DATBFZ010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 8845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8845
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2258603"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            378..1316
                     /locus_tag="VH419_12500"
     CDS             378..1316
                     /locus_tag="VH419_12500"
                     /inference="COORDINATES: protein motif:HMM:NF017652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HEY7044482.1"
                     /translation="MASGRKVIITCAVTGSGHTPSMSPHLPYTVEDVVGQSVAAAEAG
                     AAIIHLHARDPRNGKPTPDPAVFREYLKGIKDATDAVVSITSGGGTGMSVEERLQVIY
                     ETKPELCTLNLGTINYGGFPMIPRYAGSWKYDWEEPYLESTRSEPFVSTFADIEHMLR
                     DIRPRTGARFEYEAYDVGHLYTLAYYLDAGLVEPPIFIQAVMGTMGGIGAEIDHLVHL
                     KATADRLLGDSFEWSVLGGGRNQFKMVTVSALMGSHVRVGLEDGLFIGRGQLAESNAD
                     QVAKIKRILTELSLETATPDEAREILGLKGLDQVGW"
     gene            1313..2278
                     /locus_tag="VH419_12505"
     CDS             1313..2278
                     /locus_tag="VH419_12505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009078707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEY7044483.1"
                     /translation="MSVGPRPEPNAVRTVAIVGAGVIGGGWAAHFLRMGFDVVAWDPG
                     TDAQARLDRLVDTAWPSLERLGLADGARRDRLAFADSLEDALSHADFVQESSPESLGA
                     KISVLAAIDRVARPGVVVGSSTSGFGMTAMAVDCATPGRFVVGHPFNPPYLIPLVEVV
                     GGRDTDPETVGWAEKFYTQAGKVCLTLEQEVAGFVGNRLQEALWREALHMIDSGQATA
                     QQIDDSIVYGPGLRWALMGPILTFHLAGGQGGMAHMLDHFAPALLEPWTRLDAPSLTP
                     ELRDVVVAAAVEAAGGQSVAELEQRRDAFLVDLLLLLEKHRPAHG"
     gene            2271..2795
                     /locus_tag="VH419_12510"
     CDS             2271..2795
                     /locus_tag="VH419_12510"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEY7044484.1"
                     /translation="MADPGQPFSAYAAMVRPEWLDYNGHMHDASYATVLSDANEEFFD
                     WLGLSEDYRVTTGAAFYTVETHIRFLAECSAGQRLTAATLLVAADTKRIRLYTELFAD
                     DGASAAVAHEDRSVGSTLVATGESLYVHVDGAAGRVTPVPDDRQARVDDVLRAHALLP
                     RPAHLGRGIHIQPR"
     gene            2915..4078
                     /locus_tag="VH419_12515"
     CDS             2915..4078
                     /locus_tag="VH419_12515"
                     /inference="COORDINATES: protein motif:HMM:NF017102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEY7044485.1"
                     /translation="MLSNQARGQRAAVASAAARHMVLCAVALAITATSFFATPLPKAA
                     AEGGVDDYPVELKTPAQDSLADQWLFYNRECTSFVAWRLNHDAGLAFNNSYFGEHWGN
                     ASNWAYAADQVGVTVDDNPTEGAVAWWSAGSAGSSSGHVAWVRSASATEITIEEYNYL
                     SRGGYDTRTISSTSSTWPSAFIHLGDLPMWNTARPVVKGEGRVGKRLVTGEAFVGDAL
                     TAASGIWSVTPDSIRYQWQRNGVPIRGATGAAYTPTAADGGHRVSVKVTVRADGHTSA
                     SATSAPVVVRFGVATFARRPHIRGQAMIGIVQTAAAAVSPGRGHLSPNRAQFTYRWFR
                     GDHAIRRANQATYQVTGRDVGSRLRVRVRINAPNWVSSTSWSARTAAVVPRTG"
     gene            complement(4082..5785)
                     /locus_tag="VH419_12520"
     CDS             complement(4082..5785)
                     /locus_tag="VH419_12520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY7044486.1"
                     /translation="MRVLIEPRWRPVYAAFAAAAVLHLLWVRFVADAGGDLAAQDAWA
                     AFARSHPGSAYNLAWYGGMYPVSYSAISPYVMAILGVRTTMVLAGTISAAILAYLIIR
                     TGVVRRPLWPALYGAVALTGNAVSGRATFGLGVMFGLAAVACVVTWPAASRPPGSSVL
                     RTLPAAVLAALATAASPVAGLFLGIAAAALWLEGRRVDGATLGVPPVLVLAVSALLFP
                     FAGRQPMPAVSMILPVVVTLALMWLSPRSWRVVRIGSVLYVGAVFAAWVIPSPVGSNI
                     TRLGLLFGGVLVVAVAATRLPSPARLFAAVQSPSPQVTGVLAATIITSSVWQVGIAAR
                     DVMHMQPTESQSSGVQPLIDWLAGSDARLGRIEAVPTQSHREAAALAPYVNLARGWNR
                     QADEGRNPIFYEEDLLTAASYRAWLDRWAVRYVVVSSAPPDSAAVGEEALVSQGLPYL
                     RLVWASAGWTLYRVASPQPIAGRPATVTRFGAAGLVLRVPRPATVLVRIPSSPWLTLV
                     DAKDSPIDAPFEGCLSTTTVPETAELPSVTWTVLHAPHAGTYRISAPYSLARGSPCGD
                     D"
     gene            complement(6036..7052)
                     /locus_tag="VH419_12525"
     CDS             complement(6036..7052)
                     /locus_tag="VH419_12525"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HEY7044487.1"
                     /translation="MPPLRAGNVYAAAAAGGVQPRVSDEPAYIYVPNSYGAPVTTVIN
                     QRTHKIVRVLRTGALSQHVTPSYDLSTLYVESSAANRLVAINPGTARVERRIPVSRPY
                     NLYFTPDGEHALVIDEEHDRIVFTDPDTFKRQDVVGDPSCRGPNHADFSANGRYFLVT
                     CEFSGSLLKVSTLQHKVIGRLHLGPGSQPQDVRLAPDGRSFYVADMGRNLLLRIPWNR
                     LRVAGSTQTRMMPHGIYPSRDGRFLYVSNRGAGSVQVFSLARQRIVDTWRIPGGGSPD
                     MGGLSADGRTLWLSGRNDGDVYGWNTRTGRLIAKIHVGGSPHGLLVWPQPGRYSLGHT
                     GNMR"
     gene            7351..8460
                     /gene="amrS"
                     /locus_tag="VH419_12530"
     CDS             7351..8460
                     /gene="amrS"
                     /locus_tag="VH419_12530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015071173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HEY7044488.1"
                     /translation="MDDTLTLDDPFTVPTAYWHQLEDGRVQCDVCPRACKLHEGQRGL
                     CFVRARIDDQVVLTSYGRSSGFCVDPVEKKPLNHFLPGSPVLSFGTAGCNLACKFCQN
                     WDISKSREIDTLADRASPLTLAELAASTGCRSVAFTYNDPVIFWEYAADVADACHARG
                     VKAIAVTAGYMCPRPRAEFYQHIDAANVDLKAFTQDFYHRVCASNLDDVLDTLCYLRH
                     ETDVWFEITTLLIPGRNDSDEEIDAETRWIAAELGLDVPLHFTAFHPDYKMLDVPPTP
                     LATLTRARTIAMGNGLRYVYTGNVHDVAGGTTLCPGCGEPVVVRDWYDLTRYELDEHG
                     SCLRCGTALPGVYDGPAGRWGRRRLPIVATAGLRP"
     gene            8504..>8845
                     /gene="amrB"
                     /locus_tag="VH419_12535"
     CDS             8504..>8845
                     /gene="amrB"
                     /locus_tag="VH419_12535"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HEY7044489.1"
                     /translation="MGQARRAVVAGTFYPAKADELAATVDAALAAASLGQSVAGLAGV
                     IVPHAGYVYSAPVAATAYARLVGLRGRIHRVAILGPSHFVPLEGLAVPTVASFLTPLG
                     AAPVAGDACARL"
BASE COUNT         1430 a         3017 c         3067 g         1331 t
ORIGIN      
        1 tgcatgggta gccaggcaaa ccgccgccgg cgaccggtgg atgtccgaaa accgccagtc
       61 gccggccagg cccgagggac agatcgccgg ccgacctgct cagcgggcac aaacggttcc
      121 cgctcttcgc tgccgaccca cctcagccga ggcggacggt ccgccgggca acaggcccgc
      181 ccaatcgcga ccgtctccgc ccaccggaaa ctgagcgtcc cagcacctct tggcaaacca
      241 cgccatccgg atggctgccg aggcaaacca ccgctggcac gcgtacgggg cggtgcttga
      301 catccggtca gagtcggacg acggtttcac atgatgcgac gttcgctcgc atagcgctct
      361 ttctccagga gtccttcatg gcatcaggac gcaaggtcat catcacgtgc gcggtgaccg
      421 gatcaggaca tacgccgtcg atgtctccgc atctcccgta cacggtggag gacgtggtgg
      481 gtcagtcggt cgcggcggcc gaagcgggcg cagccatcat ccacctccat gcccgcgacc
      541 cgcgcaacgg caagcccaca ccggacccgg ccgtcttcag ggagtacctc aagggcatca
      601 aggacgccac cgacgcggtc gtgtccatca ccagcggcgg cggcaccggc atgtcggtgg
      661 aggagcgact ccaggtgatc tacgagacga agccggagct ctgcacgctc aacctcggga
      721 ccatcaacta cggcggattc ccgatgatcc cgaggtatgc cgggtcctgg aagtacgact
      781 gggaggagcc gtatctcgag agcacccggt ccgagccgtt cgtgagcacg ttcgccgaca
      841 tcgagcacat gttgcgggac atccggccac ggacaggtgc gcggttcgag tacgaggcgt
      901 acgacgtcgg ccacctctac acgctcgcct actacctcga cgcaggcttg gtcgaaccgc
      961 cgatcttcat ccaggccgtg atgggcacga tgggagggat cggcgcagag atcgaccacc
     1021 tcgtccatct gaaggcgacg gctgatcggc tgctcggcga ctcgttcgag tggtcagtac
     1081 tgggaggtgg ccggaaccag ttcaagatgg tcaccgtgtc ggctctgatg ggctcgcacg
     1141 tacgggtggg cctcgaggac ggtctcttca tcggccgagg tcagctggcc gagtcgaacg
     1201 ccgaccaggt ggcgaagatc aaacggatcc tgaccgagct ctcgctggag accgcgacgc
     1261 cggacgaggc gcgcgagatc ctcgggctca aggggctcga ccaggtgggc tggtgagcgt
     1321 cggcccccga ccggaaccga acgccgtcag aaccgtcgcc atcgtcggcg cgggcgtcat
     1381 cgggggcggc tgggcggcgc atttcctgcg gatgggcttc gacgtggtcg cctgggatcc
     1441 cgggaccgac gcacaggcca ggctcgatcg cctcgtggac accgcatggc cctcgctcga
     1501 gcggctcggt ctggccgacg gtgcgcggcg ggaccgcttg gcgttcgcag acagcctcga
     1561 ggacgccttg tcccacgcgg acttcgtgca ggagtcttca ccggagtcgc tgggcgcgaa
     1621 gatctccgtg ctcgccgcca tcgaccgggt tgcgcgtccc ggagtcgtcg tggggtcgag
     1681 cacgtcaggt ttcgggatga cggcgatggc ggtcgactgc gcgacacccg gccggttcgt
     1741 ggtcgggcat cccttcaacc cgccgtacct catcccgctc gtcgaggtgg tcggtggacg
     1801 ggacaccgac ccggagaccg tcggctgggc cgagaagttc tacacccagg ccggcaaggt
     1861 ctgtctcacc ctcgagcagg aggtcgcagg cttcgtcggg aaccggctcc aagaggcgtt
     1921 gtggcgcgag gcgctgcaca tgatcgactc gggacaggcg accgcgcagc agatcgacga
     1981 ctcgatcgtg tacgggccgg gcctgcgttg ggccctgatg ggtccgatcc tcacgttcca
     2041 cctggccggc gggcaaggcg ggatggccca catgctcgac cacttcgccc ccgctctgct
     2101 ggagccctgg acgcgcctcg acgcgccgtc cctgacgccg gagcttcgtg acgtcgtggt
     2161 cgccgccgcg gtcgaggcgg ccggtgggca gagcgtcgcg gagctggagc agcgacgcga
     2221 cgcgttcctc gtcgacctgt tgctgctgct ggagaagcac cgcccggccc atggctgatc
     2281 cggggcaacc gttctccgcg tacgccgcga tggtccgacc cgagtggctc gactacaacg
     2341 gccacatgca cgacgccagc tacgcgaccg tgctcagcga tgcgaacgag gagttcttcg
     2401 actggctcgg tctgagcgag gactatcgcg tcacgacggg tgcagcgttc tacaccgtgg
     2461 agacgcacat ccgctttctc gccgagtgct cggcgggcca gcggctgacg gcggcgacgt
     2521 tgctcgtggc ggccgacacg aagaggatcc gcctgtacac cgagctgttc gccgacgacg
     2581 gtgccagtgc cgcggttgcc cacgaagacc gttccgtcgg ttcgaccttg gtcgcgaccg
     2641 gtgagtcgtt gtacgtccac gtcgacggtg cggccggccg ggtcaccccg gtgcccgacg
     2701 accgccaggc tcgggtcgac gacgtactcc gagcgcacgc gctgctgcca cggccggcac
     2761 acctcggtcg cggcatccac atccagcccc gctgaggtcg cctccggcct gatgacgtca
     2821 ccggtcggtg aatggcccag aggtgggtcg gccgatcagc cgatatccga ccaaacgcat
     2881 gattcatcct gacggcccaa ctacgttccg tcagatgctc tcgaaccagg cacgtggtca
     2941 gcgcgctgca gtggcgtcgg cggccgcgcg gcacatggtg ttgtgcgccg tcgcgcttgc
     3001 gatcacggct acctctttct tcgcgacacc gctgccgaaa gcggctgccg aaggaggggt
     3061 ggacgactac ccggtcgaac tgaagacgcc cgcacaggac tcgctggccg accagtggct
     3121 gttctacaac cgcgagtgca cgtcgttcgt ggcctggcgg ctgaaccacg acgccggact
     3181 ggcgttcaac aactcctact tcggcgagca ctggggcaac gcgtcgaact gggcctacgc
     3241 cgcggaccag gtcggcgtca ccgtggacga caacccgacc gagggtgccg tcgcctggtg
     3301 gagtgccggt tcggccggct cctccagcgg gcatgtcgcc tgggtgcgga gcgcttctgc
     3361 cacggagatc accatcgagg agtacaacta cctctcccgc ggcggatacg acacgcgcac
     3421 catcagctct acgagctcga cctggccgag cgcgttcatc cacctcggcg acctcccgat
     3481 gtggaacacg gcacgtcctg tcgtcaaggg cgaggggcgc gtcggcaaac gactcgtcac
     3541 tggtgaagca ttcgtcggtg acgcgctcac cgcagcgtcg gggatctggt ccgtgacccc
     3601 ggacagcatc cgctaccagt ggcagcgcaa cggcgtaccg atcaggggcg ccaccggcgc
     3661 ggcctacaca ccgaccgcgg cggacggcgg acaccgggtg tctgtcaagg tcactgtgcg
     3721 cgccgacggt cacacgtccg ccagcgccac cagcgcgccg gtggtggtcc ggttcggcgt
     3781 cgcgacgttc gcccgccgac ctcacatccg tggtcaagcc atgatcggca tcgtgcagac
     3841 cgcggcagcg gccgtgtcgc cggggcgcgg ccatctgtcg ccgaaccgcg cccaattcac
     3901 gtatcggtgg tttcggggcg accacgcgat ccgccgcgcc aaccaggcga cgtaccaggt
     3961 gactggccgc gacgtcggct cccggctgcg tgtccgggtg aggatcaacg cgccgaactg
     4021 ggtgtcgtcg accagctggt cagcacgcac ggcagccgtc gtgccgcgta ccggctgagg
     4081 ctcagtcgtc cccgcacgga ctgcctctcg cgagggagta gggagcggag atgcggtagg
     4141 tgcctgcatg gggcgcgtgc aggaccgtcc aggtgacgct gggcagttcg gctgtctccg
     4201 gcacggtggt cgtcgacaag catccctcga acggggcgtc gatgggcgaa tccttggcgt
     4261 cgaccaaggt gagccaggga gacgacggaa tcctgacgag cacggtcgcc ggccgcggca
     4321 cccgaagcac caaccccgca gcgccgaacc gggtgacggt cgccggcctg ccggcaattg
     4381 gctgaggcga cgctacgcgg tagagggtcc aaccggcgct tgcccacacg aggcggagat
     4441 atggcagacc ctgggagacc agcgcctctt ccccgacggc tgcgctgtcg ggcggagcgc
     4501 tcgacacgac cacgtatcgc accgcccatc gatccagcca ggcccggtac gaggcggcgg
     4561 tgagcaggtc ctcctcatag aagatcgggt tgcgcccctc gtcggcctgt cggttccaac
     4621 cgcgcgccag gttgacgtac ggagcgagcg cggcggcctc gcggtggctc tgcgtcggca
     4681 cggcctcgat gcgacccagc cgagcgtccg aaccggccaa ccagtcgatg agcggctgga
     4741 cgccactcga ttgcgactcc gtcggctgca tgtgcatcac gtcccgggcg gcgataccga
     4801 cctgccacac cgaggacgtg atgatcgtgg cggcgaggac accggtgacc tgcggtgagg
     4861 gcgactggac cgccgcgaac aaccgtgccg gcgagggaag tcgggtcgca gcgacagcga
     4921 cgaccagcac gcctccgaag agcagaccga gccgggtgat gttgctgccg accggcgagg
     4981 gaatcaccca cgccgcgaag accgcgccga catacagcac cgaaccgatc cgcacgacgc
     5041 gccacgagcg gggggacagc cacatcagcg ccagcgtgac gacgaccgga aggatcatcg
     5101 agaccgcggg catcggttgc cttccggcga aggggaacag caacgccgac acggcgagaa
     5161 ccagcacggg cggcaccccg agggtggcgc cgtccacgcg gcggccttcg agccaaagtg
     5221 ccgcagccgc gatgccgagg aacagaccag cgacggggct cgccgccgtc gccagcgccg
     5281 ccagcaccgc ggccggcagg gtacgtagca cgctcgagcc gggtggtcgc gaagccgccg
     5341 gccaggtgac cacgcacgcg accgcggcca ggccgaacat cacgccgagc ccgaaggtgg
     5401 cgcgtcccga tacggcgttg ccggtcagcg cgaccgcgcc gtacaacgcc ggccacagcg
     5461 gccggcggac gactccggtg cggatgatga ggtaggcgag gatcgcggcg gagatggttc
     5521 cggccagcac catcgtcgtt cgtacgccga ggatcgccat cacgtacggc gagatggcgc
     5581 tgtacgacac cgggtacatc ccgccgtacc acgcgaggtt gtaggcactt cctgggtgtg
     5641 accgcgcgaa cgcggcccac gcgtcctgtg cggcgagatc gcccccggcg tcggcgacga
     5701 atcgaaccca cagcaggtgc agcacagccg ccgctgcgaa cgcggcatac acaggtctcc
     5761 agcgtggctc gatgaggacg cgcaccttcc aagcatgccc tgaccggccc gtacctaccc
     5821 ggtcagcccc gccgttactc cgatcacaag atctcggcgg tccggcttcg cgcagctcga
     5881 cgcacgggtg gaaacgggct gttgccgtcc ggtgtgcccg aatttgtcct cgatggctgg
     5941 gaggtgcgca ggccgagccg aagcgtgatg agcccgttca tccgtcttca cctcgaagca
     6001 ggattcgtac gccgctgtgg tgacagccgg caggatcacc gcatgttgcc ggtgtgaccg
     6061 agggagtagc ggcccggctg cggccagacg agcagcccgt gcgggctgcc accgacatgg
     6121 atcttggcga tgagccggcc ggtccgggtg ttccagccgt agacgtcgcc gtcgttgcgg
     6181 cccgacagcc agagggtccg gccgtcggcg gagagtccgc ccatgtcggg gctgccgccg
     6241 cccgggatcc gccaggtgtc cacgatccgc tgccgtgcca gcgagaacac ctggacgctg
     6301 ccggcgccgc ggttggagac gtagaggaaa cgcccgtccc ggctggggta gatgccgtgc
     6361 ggcatcatcc tggtctgggt ggacccggcg actcgcaggc ggttccacgg gatgcggagc
     6421 agcaggttgc ggcccatgtc ggcgacgtag aagctgcgcc cgtcgggtgc gagccggacg
     6481 tcctgtggct ggcttccagg tccaagatgc agtcggccga tcaccttgtg ctgcagcgtg
     6541 gacaccttca gcagggagcc ggagaactcg caggtgacga ggaagtatcg cccgttcgcg
     6601 gagaagtcgg cgtggttcgg gccccggcag ctcgggtcgc cgacgacgtc ctgacgcttg
     6661 aacgtgtcgg ggtcggtgaa gacgatccgg tcgtgctcct cgtcgatcac cagtgcgtgt
     6721 tcgccgtccg gcgtgaagta gaggttgtag gggcgcgaca cggggatccg tcgttccacc
     6781 ctcgcggtgc cagggttgat cgcgaccaac cggttggcgg cgctggactc gacgtacaac
     6841 gtgctcaggt cgtacgacgg cgtgacgtgc tgcgacagcg cgccggtccg gagcacacgc
     6901 acgatcttgt gcgtgcgctg gttgatcacc gtggtgaccg gagcgccgta gctgtttgga
     6961 acgtagatgt acgcaggttc gtctgaaacg cgcggctgga cgccccctgc cgcggcggcg
     7021 gcgtacacgt tcccggctct gagcggaggc atgccgggga tcggatgtcg gaacggcttc
     7081 ggcggccgga ccgtgatcct gcccccgagc gccggctccg aggtggcgcc tgcggacggc
     7141 gaggtgctgg gaggcctgga cgaccggacg gactgagacg gtgtcgagtg gtcgtcgctc
     7201 gacgacgtgc acgcgacagc tgacgctgcc accgtcagca gcgccaccgc cttcgtgagt
     7261 agcgccctcg tcaccgccat gcctcacgct gggaacctgt cactgacgag tacacctcat
     7321 cccgggctac cgtgggggca gaggccggac atggacgaca cgctcaccct cgacgacccg
     7381 ttcaccgtcc cgacggcgta ctggcaccag ttggaagacg gccgggtcca gtgtgacgtc
     7441 tgcccgcgcg cgtgcaagct gcacgagggc cagcggggtc tgtgcttcgt gcgtgcgcgg
     7501 atcgacgacc aagtcgtgct gacgtcgtac ggccgctcga gcgggttctg cgtcgacccg
     7561 gtggagaaga agccgctcaa ccacttcctg cccggctcgc cggtgctgtc gttcggcacg
     7621 gcgggatgca acctcgcctg caagttctgc cagaactggg acatctccaa gtcccgggag
     7681 atcgacacgc tcgccgaccg cgcgtcgccg ctgacgctcg ccgagctcgc ggcgtcgacg
     7741 gggtgtcgga gcgtggcgtt cacctacaac gaccccgtca tcttctggga gtacgccgcc
     7801 gacgttgccg acgcgtgcca cgcgcggggc gtcaaggcga tcgcggtcac ggcggggtac
     7861 atgtgccctc ggccgcgagc ggagttctac cagcacatcg acgccgccaa cgtcgacctc
     7921 aaggcgttca cgcaggactt ctaccaccgg gtctgtgcat cgaacctcga cgacgtactc
     7981 gacacactct gttacctacg gcacgagacc gacgtctggt tcgagatcac gacgctgctc
     8041 atccccggcc gcaacgacag cgacgaggag atcgacgccg agacgaggtg gatcgcggcc
     8101 gagctcgggc tcgacgtacc actgcacttc accgccttcc atcccgacta caagatgctc
     8161 gacgtgccac cgacgccgct ggccacgttg acgcgggcgc ggacgatcgc gatgggcaac
     8221 gggctgcggt acgtctacac cggcaacgtg cacgatgttg cgggcggtac gacgctctgc
     8281 ccgggctgcg gtgaacccgt cgtggtccgg gactggtacg acctgacgcg atacgagctc
     8341 gacgagcacg ggagctgcct gcggtgcggc acggcgcttc cgggcgtgta cgacgggcct
     8401 gccggccgct gggggaggcg ccggctcccc atcgtcgcca cggccgggtt gcgtccgtga
     8461 cggcggctgc cgggacaacc gccggcacgg acgtaagtac gtgatggggc aggctcgtcg
     8521 cgccgtcgtg gcgggcacgt tctaccctgc caaggccgac gagctcgccg ccacggtcga
     8581 cgccgcgctg gccgcggcga gcctcggcca aagcgtcgcg ggcttggccg gcgtgatcgt
     8641 gccgcatgcg gggtatgtgt actccgcccc ggtggctgcc acggcgtacg cccgcctggt
     8701 cgggctgcgt ggccgcattc accgggtcgc gatcctcggg ccctctcact tcgtcccgct
     8761 cgaaggcctc gccgtgccga ccgtcgcctc cttcctgaca cccttaggag ctgctcccgt
     8821 cgccggcgat gcctgcgccc gtctg
//
LOCUS       DATBFZ010000415         3839 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_6381308, whole genome shotgun sequence.
ACCESSION   DATBFZ010000415 DATBFZ010000000
VERSION     DATBFZ010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 3839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3839
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6381308"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            153..551
                     /locus_tag="VH419_12540"
     CDS             153..551
                     /locus_tag="VH419_12540"
                     /inference="COORDINATES: protein motif:HMM:NF018663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY7044490.1"
                     /translation="MTQELRTFLTFQGGTATAALDLYRDIFDDFELIEIDHYGAEDVG
                     PEGTVKVAKFRLAGSDFSCADSPIRHEWGFTPAVSLWIECADRDELQRLFDGLSDGGN
                     VYMPLDDYGFSTRFGWVGDRFGVTWQLNVP"
     gene            610..2451
                     /locus_tag="VH419_12545"
     CDS             610..2451
                     /locus_tag="VH419_12545"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEY7044491.1"
                     /translation="MRCRFVPFVPGRRPTDEDKRSGLGSSDSVALVTGGDGDNRTVLL
                     FEVSELPHGASVEAKITVDDRPLPGWEHRQVTLTQSGRQRLEVRPEHDLADVDLGFNQ
                     GFLRTARVQLHVLSGGAVTTADETSLDVCDVRNLGTLYKRLIDRLVRVDTIRQAGRAR
                     VMDPGLQYHPWYPVLTIGGDKAALYIEALVADIVGKEYHLTDPAWLLRVGVYLELLTG
                     IGIIEAVRSEVGDLLRPEERAALQRSDAFREIRRHINPEGWREVWAHRRICFPRFGSP
                     RLGPVSALNLMRKRDATLRFLHVHHEDLKHAIELAGPNQFNSQETWQRVFRDAERAVM
                     RQTAHAFPELGFLPSAAREVVLWQRLGFAGQQGVYPTACNQYRASMNSVADWAKRRGL
                     MDHAGVQCVPSGASLFEALVHDPQRVEVLQRQDRLGPDLTVVEPIPESEPSIEEIERL
                     LAEVPIFRMLSPAEVHTLALGVRPLFLGPTQRFIVEGREGTSLFLVGEGEVEVRRRNA
                     DGTDWLVETLGRGQVVGEMALLTGRRRNATVRSVDESLVLEIGFEQYKPLLLAHPEWL
                     EELAAIMQARLANSRVRKAGRHDAAPSPLLAQIRRNFFGGLAAAGPD"
     gene            complement(2494..2832)
                     /locus_tag="VH419_12550"
     CDS             complement(2494..2832)
                     /locus_tag="VH419_12550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044492.1"
                     /translation="MLYAPIAYGDDHIDGPARAIRCALAIGERTSQRLGIAVRAGLHT
                     GEIELVDDDIAVHIAARVAALAAPGQVLVSRAVTDLVAGSAIEFRDEGPHQLKGVPGS
                     WPLFAAGAPG"
     gene            complement(3009..3428)
                     /locus_tag="VH419_12555"
     CDS             complement(3009..3428)
                     /locus_tag="VH419_12555"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020392399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HEY7044493.1"
                     /translation="MTPTHTPTVLFLCVHNAGRSQMAAGWLRHLGGDRVIVYSGGSEP
                     ARDVNPTAVEAMAEVGIDIRHEVPKLWTNDIIRAADVVITMGCGDACPFYPGRRYEDW
                     ELDDPAGLDLPAVRRIRDRIRQRVETLIAELGVATDA"
     gene            complement(3491..>3839)
                     /locus_tag="VH419_12560"
     CDS             complement(3491..>3839)
                     /locus_tag="VH419_12560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020392398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HEY7044494.1"
                     /translation="AVNVSTTTRSSGALWLSEVVATAGLLLVINGCVRTGRSDVVAVA
                     VGVWIGGAYFFTSSTSFANPAVTIGRTLSDTFAGIAPASAPMFIVMQLAGAGVAYGLI
                     RYLFRPAEAGRES"
BASE COUNT          599 a         1222 c         1381 g          637 t
ORIGIN      
        1 acctgcgcgc ctgtgtccac acgggcgcca ggtgcgtgta cgtccctgcg gagcctgtgc
       61 gctgggagct gtgacgaggt aggggtcgtg ccggtcacag tgcggcaacg agtccggacc
      121 agctgcgacg tggggtcgtt agtgttcgtc gtatgacgca ggaattgcga accttcctga
      181 cgttccaggg tggcaccgcc actgcggcgc tcgacctcta ccgcgacatc ttcgacgact
      241 tcgagctcat cgagatcgat cactacggcg cggaggatgt cgggcccgag ggcacggtga
      301 aggtggcgaa gttccgtctc gctggtagcg acttcagctg cgccgacagt ccgatccgtc
      361 atgaatgggg cttcacgcca gcggtgtcac tgtggatcga atgcgccgac cgcgacgagc
      421 tccaacggct cttcgatgga ttgagcgacg gcggcaacgt gtacatgccg ctcgacgact
      481 atgggttcag tacgcgtttc ggctgggttg gcgaccgctt cggcgtgacc tggcaactca
      541 acgtgccgta acgacagaca actccgctac caagcaagcc gactggcgct cggccatact
      601 ttcccgccag tgcgatgtcg cttcgtaccc ttcgtccccg gacgccgacc gaccgatgaa
      661 gacaagcgaa gtggcctggg ctcgagcgac agtgtcgccc tcgtcaccgg tggggatggg
      721 gacaaccgga cggtcctgtt gttcgaggtg tcagagcttc cccacggcgc gtcggtagag
      781 gcgaagatca ccgtcgacga ccgcccgctg ccgggatggg aacaccggca ggtcacgctc
      841 acgcagtccg gccgccagcg cctcgaggtg cggcccgagc acgacctcgc tgacgtggac
      901 ctcgggttca atcaggggtt tctgcgcacc gcgcgggtcc agctccatgt cctcagcggc
      961 ggcgcggtca cgacggccga cgagacgtcg ctggacgtgt gcgacgtgcg caacctcgga
     1021 accctctaca agcgactgat cgaccggctg gtccgggtgg acacgattcg ccaggcggga
     1081 cgggctcggg tcatggaccc gggtctgcaa taccacccgt ggtaccccgt gctcaccatc
     1141 ggtggggaca aagccgcgct ctacatcgag gcgctcgttg ccgacatcgt cggcaaggag
     1201 taccacttga ccgacccggc gtggctcctt cgggtcggcg tctacctcga gctgctgacc
     1261 gggatcggca tcatcgaagc ggtgcgctca gaggtcggtg acctgctccg gccggaggag
     1321 cgggctgcgc ttcagaggag cgacgcgttc agggagattc ggcgccacat caacccggag
     1381 ggctggcggg aggtgtgggc gcaccgccgc atctgcttcc cgaggttcgg gagcccccgc
     1441 ctcgggccgg tgtcggcgct caacctcatg cgcaagaggg acgcgaccct ccggttcctc
     1501 cacgtgcacc acgaagacct gaagcacgcc atcgagttgg ccggtccgaa ccagttcaac
     1561 tcgcaggaga cgtggcagcg ggtgttccgc gatgctgagc gggccgtcat gcgccaaacc
     1621 gcccatgcgt tcccggagct cgggttcctc ccctcggcgg cccgagaggt ggtgctctgg
     1681 cagcgactcg gcttcgctgg tcagcagggc gtctacccaa cggcctgcaa ccagtacagg
     1741 gcctcgatga actcggtcgc agattgggcg aagcgccgag gtctgatgga ccacgcgggt
     1801 gtccaatgcg tgccgtccgg ggccagcctg ttcgaggcct tggtccacga cccgcaacgg
     1861 gtggaggtgc tccaacggca ggaccgcctc gggccggatc tcaccgtcgt ggagccgatc
     1921 cctgagagcg aaccgagcat cgaggagatc gagcgccttc tcgccgaggt gccgatcttt
     1981 cggatgctgt cccccgcgga ggtgcacacc ttggcccttg gcgtccggcc cttgttcctc
     2041 ggtcccaccc aacggttcat cgtcgaggga cgcgagggca cgtcgctgtt cttggtcggc
     2101 gagggcgagg tcgaagtgcg ccgccggaac gcggacggca ccgactggct ggtcgagacc
     2161 ttgggccgag ggcaggtcgt cggcgagatg gccctcttga ccggtcgacg tcggaacgcg
     2221 accgtacggt cggtcgacga gagcctggtc ttggagatcg gctttgagca gtacaagccg
     2281 ttgctgctgg cgcatcccga gtggctcgag gagctggccg ccatcatgca ggcacggttg
     2341 gccaacagcc gggtccgcaa ggccgggcgg cacgacgccg caccgtcgcc gctgctggcg
     2401 cagatccggc gcaacttctt cggcggtctt gcagcagcag gtcctgactg agacttcggc
     2461 ccgcaggtcg gtctgttgta cgagcgtcgt ggttcagccg ggtgcgccgg cagcgaagag
     2521 cggccagctt ccgggtacgc ccttgagctg gtgcgggccc tcgtcgcgaa actcgatggc
     2581 ggagccggcg acgaggtcgg tgaccgcgcg ggagacgagg acttgtcctg gcgcagcgag
     2641 cgcggcgacc cgcgctgcga tgtggacggc gatgtcgtcg tcgacgagtt cgatctcgcc
     2701 cgtgtgcaat ccggcgcgca ctgcgatccc gaggcgttga gatgtccgct cgccgatggc
     2761 gagcgcgcat cggatcgccc gcgctggtcc gtcgatgtgg tcgtcgccgt acgcgattgg
     2821 cgcatagagc acgagcgccc gtgtgcgttc ggggtatgtc gccgcgaaca gccgacgtca
     2881 ccgcggcgcc accggctcca ccgaagatcg cctcgctgtg ccgccagatc gtcgcttcac
     2941 cctggcgcgg aatgaggtgc tgagtcttcc gatgggggtc gctcggagga cggactcgtc
     3001 tcgccgtctc aggcgtcggt ggcgacgccg agctcggcga tgagggtctc gacgcgttgt
     3061 cggatccggt ctcggatgcg tcgcacggcg gggaggtcga ggccggcagg gtcgtcgagc
     3121 tcccagtctt cgtatcgtcg gccggggtag aaggggcagg cgtcgccgca gcccatggtg
     3181 atgacgacat cggcggcgcg gatgatgtcg ttggtccata gcttcgggac ctcgtggcgg
     3241 atgtcgatgc cgacctcggc catcgcctcg acggcagtgg ggttgacatc gcgggccggt
     3301 tcggatcctc cggagtagac gatcacccgg tcaccgccga ggtgacggag ccagccggcg
     3361 gccatctggg agcggccggc gttgtgcacg cacaggaaca ggacagtcgg cgtgtgggtg
     3421 ggtgtcacgg cttcgcgtcg tggatcgcgc tgtgccggtg ggggaccatc ctcggtgggc
     3481 ggagcggcgt tcacgactcg cgtccggcct cggccggtcg gaacaggtag cggatcagtc
     3541 cgtaggcgac gccggccccg gcgagttgca tgacgatgaa catcggcgcc gatgcgggtg
     3601 cgatcccagc gaaggtgtcg gacagggtgc ggccgatggt gacggcgggg ttggcgaagc
     3661 tcgtggagga ggtgaagaag taggcgccgc cgatccacac cccgacggcg acggcgacga
     3721 cgtcggaccg acctgtgcga acgcaaccgt tgatgacgag cagcagcccg gcggtggcga
     3781 ccacctcgga gagccacagc gctccggacg accgcgtcgt ggtcgacacg ttcacggcg
//
LOCUS       DATBFZ010000416         4997 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_286404, whole genome shotgun sequence.
ACCESSION   DATBFZ010000416 DATBFZ010000000
VERSION     DATBFZ010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 4997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4997
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286404"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..672)
                     /locus_tag="VH419_12565"
     CDS             complement(<1..672)
                     /locus_tag="VH419_12565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017934341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEY7044495.1"
                     /translation="MTLTAPATPRDADLDWGGHDRTRRLAWTLLAVLAVASIASVLIG
                     SRMVSPAAVFDTGHAGHVVAMSRIPRTLVALSVGAALGLAGACMQGLTRNPLADPGIL
                     GINAGASFAMVLAITVFGVSALSAYVWFAFAGAALAMVLVHGIAAFGRDGATPAKLTI
                     AGAALTAALSSWISAALLASRDTLESFRFWQVGTVGGRGYDVLLTVLPFLVVGALLAF
                     ASSQRL"
     gene            complement(669..1694)
                     /locus_tag="VH419_12570"
     CDS             complement(669..1694)
                     /locus_tag="VH419_12570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008354512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HEY7044496.1"
                     /translation="MVRLRILVACVAVALALTSCSTSTTDDDSDPKAATSVDPHAFPV
                     TIEHAYGTTTIDREPKRVVTLGWSDQDVALALGVVPVGAVKITWGGNAKDSTPWFDAK
                     LAELGGNQPTRYSDADGAPIEDIAKLAPDLILATNSGITKKEYDKLSKLAPVVAFPGD
                     PWVTSWQKSLEMIGEALGRSTLAQQVEEQTDKSIVDAAKANPEIVGKSFIFAALTTTD
                     MSKIDYYTPEDNRPRLLTDLGMVNAPIIEQISKPGTFYGTISAERADELTSDVFITYA
                     ETSKDLATFEHNDLLGQIPGIKDGHVLASTNQTDALGLSAPSPLSIPYAMTHFVPQVA
                     EAAGAPS"
     gene            complement(1857..2231)
                     /locus_tag="VH419_12575"
     CDS             complement(1857..2231)
                     /locus_tag="VH419_12575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020578048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044497.1"
                     /translation="MTSGDPLLTARARVLADLAAQGLIDPHTVSLVEECVSQRRWWLS
                     QWAQGAPFVDGLVAQDVQDMLFDESQRWPVCSGCADPVEHSLSIEPELGPDPHWVCSE
                     SGHVVAPLGALPALANRTDDNR"
     gene            2259..3002
                     /locus_tag="VH419_12580"
     CDS             2259..3002
                     /locus_tag="VH419_12580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020391888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HEY7044498.1"
                     /translation="MNPSPGEPLVEVVALHPHDAERAQEGGAHRLHAAALGQHGLLSM
                     EPGSVSAIVRSTDLPIRCTLRLSDGFTTQGGEFARLRGLVADYLALGVEGFVFGFLTT
                     DLEVDVDTCAALAEALMGSPWTFDRSFDHALEAPRSARRVSELPGVDGIHTAGAVLGM
                     DTGFDDLVALAQASPRFASMAIAADGVRPEHIPWLVRSGIRAVHLGVEVRPGASWSKS
                     YVDAGFVRSWRLLLDDACGVRRDADTGTA"
     gene            3004..4206
                     /locus_tag="VH419_12585"
     CDS             3004..4206
                     /locus_tag="VH419_12585"
                     /inference="COORDINATES: protein motif:HMM:NF014114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4031 domain-containing protein"
                     /protein_id="HEY7044499.1"
                     /translation="MTILIDPPAWPAHGRLWSHLISDTSLDELHAFAAQHGIPRRGFE
                     RDHYDVPADAYPRLVRAGAVEVSSREVVSRLAAAGLRRRKATAMARRAVGNALLRPPR
                     LEAGDVVAVVATSGVVPADRLHVGVDRLRAWGLEVRLSPHVLSEHPRLGYLAALDEDR
                     AADFTEAWLDPTVSAVWMARGGYGVQRIVDLLDWRRLAEADPKVLVGFSDITGLHQAV
                     ATRLGLVTVHGHVVTSLGASTEASAEELRRLLMEPDTVVDLLAGQSTSPMVGGTATGI
                     LVGGNLAMLAADVGTATSRPAKDGIVVLEDVDEYPFRVDRMLTQLIRAGWFAGVAGIV
                     VGAFTDCGEPAELETVLDDRLRPFGVPAVRGFDLGHTSSSAAVPLGVEATLDADAGAL
                     VLSTPALA"
     gene            complement(4211..4831)
                     /locus_tag="VH419_12590"
     CDS             complement(4211..4831)
                     /locus_tag="VH419_12590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017932910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent phosphohydrolase"
                     /protein_id="HEY7044500.1"
                     /translation="MNLPGETALSRWERDFPGRSALGQELVRRYGSVSRSYHDARHLI
                     EVLDAVDAMAAEADDIDAVRLAAWFHDAVYDVRAGDNEQRSAELAESVLPAYDVDEHT
                     VAEVVRLVRLTESHDPEPGDNNGSVLCDADLAILASDEGRYATYVDGVRAEYRHVDDR
                     TFAIGRAAVLRHLLGLTSLYRTKYGQATWEPQARANVQRELEELTY"
BASE COUNT          738 a         1668 c         1790 g          801 t
ORIGIN      
        1 gagccgctgg ctgctcgcga acgcaagcaa cgcgccgacc acgaggaacg gcagcaccgt
       61 gagcaacacg tcgtagccgc gaccgccgac cgtgccgacc tgccagaagc ggaacgactc
      121 caaggtgtcg cggctcgcca gcagcgcagc ggagatccag ctcgacaagg ctgccgtcag
      181 tgcggcaccc gcgatcgtga gcttggcggg cgtggcgccg tctcgaccga acgccgcgat
      241 gccgtgcacc aacaccatcg ccagggcggc gccggcgaag gcgaaccaga cgtacgccga
      301 caacgcggag acgccgaaca cggtgatggc gagcaccatc gcgaaggacg caccggcgtt
      361 gatgccgagg atgccgggat cggccagcgg gttgcgggtc aggccttgca tgcaggcgcc
      421 ggcaagtccc agcgcggcac cgacggacaa cgccacgagc gtccgtggga tgcgagacat
      481 ggccacgacg tgtcccgcat ggccggtgtc gaacaccgca gcgggcgaga ccatccgcga
      541 cccgatcagc accgacgcga tcgaggccac cgcgagtacg gccagcagcg tccaggcgag
      601 ccgccgggtc cggtcatgtc cgccccagtc gagatcggcg tctcgtgggg tggcgggcgc
      661 cgtcagcgtc acgacggtgc gccggcggcc tcggcgacct gcggcacgaa gtgcgtcatc
      721 gcgtagggga tcgacaacgg cgagggcgcg gacagtccga gcgcgtcggt ctgattggtc
      781 gaggcgagca cgtggccgtc cttgatgccg ggaatctggc cgagcaggtc gttgtgctcg
      841 aaggtggcga ggtccttgct cgtctcggcg tacgtgatga acacgtccga cgtcagctcg
      901 tcggcccgtt cggcggagat cgtgccgtag aaggtgccag gcttgctgat ctgctcgatg
      961 atcggcgcgt tgaccatacc gaggtcggtg agcagccggg ggcggttgtc ctcgggtgtg
     1021 tagtagtcga tcttcgacat gtcggtcgtc gtgagtgcgg cgaagatgaa cgacttgccg
     1081 acgatctccg ggttggcctt ggctgcgtcg acgatcgact tgtcggtctg ctcttcgacc
     1141 tgctgtgcaa gggtcgaccg gccgagagcc tcgccgatca tctccagcga cttctgccag
     1201 ctcgtcaccc aggggtcccc tgggaaggcg acgaccggag cgagcttgga gagcttgtcg
     1261 tactccttct tcgtgatgcc ggagttggtg gccaggatca ggtcaggcgc gagcttggcg
     1321 atgtcctcga tgggtgcgcc gtcggcgtcg gagtaccgtg tcggctggtt gccgccgagc
     1381 tcggcgagct tggcgtcgaa ccacggggtg gagtctttcg cgttgcctcc ccaggtgatc
     1441 ttgaccgcgc ccacgggtac gacgcccagc gccagcgcga cgtcctggtc ggaccagccg
     1501 agcgtgacga cccgtttcgg ctcacggtcg atcgtcgtcg taccgtaggc gtgctcgatc
     1561 gtcaccggaa aggcgtgggg atcgaccgag gtcgccgctt tcgggtccga gtcgtcatcg
     1621 gtcgtcgagg tcgagcagct cgtcagcgcc agtgccaccg cgacgcaggc gacgaggatc
     1681 ctgaggcgca ccatcgcacg cgtcctctct gaggggagtt aggcgagcct aacctaacat
     1741 caggtgagga ccagtgtgca aggccgcaac cacatgtccc ggttcgtgga gccacaactc
     1801 acggttcgat gaaccacaac tcacggttcg acgaaccaca actcacggtt cgacggtcag
     1861 cggttgtcgt cggtgcggtt ggcgagggcg ggaagggcgc cgaggggggc gacgacatgg
     1921 ccggactccg agcacaccca gtgcgggtca ggaccgagct cgggctcgat cgacagactg
     1981 tgctcgaccg ggtcggcgca accggagcac accggccagc gctgggactc gtcgaacagc
     2041 atgtcctgca cgtcctgtgc gacgagtccg tcgacgaacg gtgcaccctg cgcccactgc
     2101 gagagccacc accgccgctg cgacacgcac tcctcgacga gagagacggt gtgcggatcg
     2161 atgagaccct gggccgcgag atcggcgagg acgcgagctc gcgcggtcaa cagcgggtct
     2221 cccgatgtca tggcctccat cctggcactg ttgatcaaat gaacccgtca cccggcgaac
     2281 cgctcgtcga ggtcgtcgcg ctgcacccgc acgatgccga acgagcccag gaaggcggcg
     2341 cgcaccgact gcacgccgcg gcgctgggcc agcacggact gctgtcgatg gaaccggggt
     2401 cggtcagcgc gatcgtgcgc agtaccgacc tgccgatccg gtgcacgctg cgcctgagcg
     2461 acggcttcac cacgcagggc ggggagttcg cgcggttgcg cggactcgtg gcggactatc
     2521 tcgcgctggg cgtcgagggg ttcgtgttcg gcttcctcac cacggacctg gaggtggacg
     2581 tcgacacctg cgcggcgctc gcggaggcgc tgatgggctc cccgtggacg ttcgaccgct
     2641 cgttcgacca cgcgctcgag gccccgagga gtgcccggcg ggtcagcgag cttcccggcg
     2701 tggacggcat ccacacggcg ggtgccgtgc tcggcatgga caccggcttc gacgacctgg
     2761 ttgcgctggc gcaggcgtcg ccgcggttcg cttcgatggc gatcgcggcc gacggcgtac
     2821 gtcccgaaca catcccctgg ctggttcgct ccggcatccg cgccgtccac ctcggcgtcg
     2881 aggtccggcc cggcgcgagc tggtcgaagt catacgtcga cgccggcttc gtgcggtcgt
     2941 ggcggctgct gctcgacgac gcctgcggag tgcgccggga cgccgacacc gggaccgcct
     3001 gacgtgacga tcctcatcga cccgccggcc tggcctgccc acgggcgact gtggtcgcac
     3061 ctgatcagcg acacctcgct cgacgagctg cacgcgttcg cggcgcagca cgggatcccg
     3121 cggcgtggtt tcgaacgtga ccactacgat gtgcccgccg acgcgtaccc ccggctcgtg
     3181 cgggcgggcg cggtcgaggt ctcctcccgc gaagtggtct cccggttggc cgcggccggc
     3241 ctgcgtcgac gcaaggcgac ggcgatggcg aggcgggccg tcggcaacgc gctgctgcgc
     3301 ccgccgcggc tcgaggcggg tgacgtcgtc gccgtcgtcg ccacctcagg cgtcgtaccc
     3361 gcggatcgac tgcatgtcgg tgtcgaccgg ttgagggcat gggggctcga ggtccggctt
     3421 tcgccgcacg tgctgtcgga acacccgcgc ctcgggtacc tggccgccct cgacgaagac
     3481 cgggcagccg acttcaccga ggcctggctc gatccgacgg tctctgccgt gtggatggcg
     3541 cggggtgggt acggcgtgca gcggatcgtc gacctgctcg actggcgtcg tctcgccgag
     3601 gccgatccga aggtgctcgt cggtttctcc gacatcaccg gtctccacca ggcggtggcg
     3661 acgcggctcg gtctcgtcac ggtccatggc cacgtcgtga cgtcgcttgg tgcctcgacc
     3721 gaggcgagtg ccgaggagct gcggcgcctg ttgatggagc ccgacacggt ggtggacctg
     3781 ctggcgggtc agtcgacgag cccgatggtc ggcgggaccg ccaccggaat cctcgtgggt
     3841 ggcaacctgg cgatgctcgc cgccgacgtc ggtacggcga cgagccgtcc ggccaaggac
     3901 ggcatcgtcg tgctggagga cgtggacgag tacccctttc gggtcgaccg catgctcacc
     3961 cagctgatcc gggccgggtg gttcgccggt gtcgccggga tcgtcgtcgg ggccttcacc
     4021 gactgtggag agccagccga gctggagacc gtgctcgacg accggctgcg cccgttcggc
     4081 gtaccggccg tgcggggctt cgacctcggg cacacatcat cgagcgccgc cgtgccgctg
     4141 ggggtggagg cgacattgga cgccgatgcg ggtgcgttgg tgctgtcgac accggctttg
     4201 gcgtgagcgg tcagtaggtc agctcctcga gctcgcgctg cacgttggcg cgtgcttgcg
     4261 gctcccacgt cgcctgcccg tacttcgtgc ggtacagact ggtcaggccg aggaggtgcc
     4321 gcagcaccgc cgcccgcccg atggcgaacg tccggtcgtc gacgtgccgg tactccgcgc
     4381 ggactccgtc gacgtacgtc gcgtagcgtc cctcgtcgct ggcgaggatc gccaggtcgg
     4441 cgtcgcagag gacggaaccg ttgttgtcgc cgggttcggg atcgtgcgac tccgtgaggc
     4501 gcaccaggcg gacgacctcg gcgacggtgt gctcgtcgac gtcgtacgcc gggaggaccg
     4561 actcggccag ctccgcagac cgctgctcgt tgtcgcccgc ccggacgtcg tacacggcgt
     4621 cgtggaacca ggcggcgagc cgcaccgcat cgatgtcgtc ggcctcggca gccatcgcgt
     4681 cgaccgcgtc gagcacctcg atcaggtggc gggcgtcgtg gtagctgcgg gaaaccgagc
     4741 cgtatcggcg tacgagctcc tgtcccagcg ccgatcgtcc gggaaagtcc cgttcccaac
     4801 gtgacagtgc cgtctcgcct ggaaggttca cagctccatc atggcccgtc agcacgagac
     4861 gagtgattcc aagggaggtt gcctggctcg cggctccccg catcccgcct ggctgcgttg
     4921 tcgtcactcc gaataggagc tccgctatga gtcgctcctc cgccttgcca gatcgggcgc
     4981 ggatgagccg ctcgctt
//
LOCUS       DATBFZ010000417         1877 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_2911218, whole genome shotgun sequence.
ACCESSION   DATBFZ010000417 DATBFZ010000000
VERSION     DATBFZ010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2911218"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="VH419_12595"
     CDS             complement(<1..511)
                     /locus_tag="VH419_12595"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HEY7044501.1"
                     /translation="MPGQEPPEQTDVTPDDTIMPPAAPPASTPDAITAAKAPGREPYA
                     VVGRPRNEGHGKRLTAAFEALEVFPALAESRNRLVALMAEEHPSTGEVVRAIESDVAL
                     VIAVLRLANGLDSPKRGQVESIVSAVEVLSPEAVQRLAADAETFEFFERGKTWDAAPE
                     RFRLHAVAVQ"
     gene            complement(583..>1877)
                     /gene="gltX"
                     /locus_tag="VH419_12600"
     CDS             complement(583..>1877)
                     /gene="gltX"
                     /locus_tag="VH419_12600"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEY7044502.1"
                     /translation="NARTALYNWLLARGQGGQLVLRIEDTDRERSTPENVQTIFDALE
                     WLGIDWDEGPTYQSQNADRHRAVVEQLLADGHAYRTSAGPDEVKAFKERYGDRGFRGE
                     DEGSGAVRLHVPDEGVTVVTDVIRGESAFENSLLDDLVIARADGSAVYHLAVVVDDAD
                     AGITHVVRGADHYSNTPKHMLIQNAIGAPTPVYGHLPLLHGPDGKKLSKRHGATSVQD
                     LRDAGYLPEAVINYLALLGWGYDEETTFHTIQELQERFRLERVSKSPAVFDEQKLRWM
                     NGRYVRELDVDDLTARLEDLTGRSGLRGAVEIAQDKISTLAEFWPLTQFFFDGPADDP
                     AAREKVLEAPGAKDALSAVRAALADVPVPWTEENLESALGDVAERLGVKRKQVFQPLR
                     VAVTGTTVSPGIFETVALLGRDETLARVDHALNGRKGT"
BASE COUNT          265 a          657 c          640 g          315 t
ORIGIN      
        1 gctggacggc gacggcgtgc aggcggaagc gctcgggagc ggcgtcccac gtcttgccgc
       61 gctcgaagaa ctcgaacgtc tcggcgtcgg cggccaggcg ctgtaccgcc tcaggcgaga
      121 gcacctcgac ggcggagacg atcgactcga cctgaccacg cttcggcgag tcgaggccgt
      181 tggccagccg cagcacagcg atgaccagcg cgacgtcgga ctcgatggcg cggacgacct
      241 cgccggtcga cgggtgctcc tcggccatca gcgcgacgag ccggttgcgc gactcggcga
      301 gcgccgggaa gacctcgagc gcctcgaacg ccgcggtcag gcgcttgccg tgaccctcgt
      361 tgcggggccg gccgacgacg gcgtacggct cgcggccagg ggccttcgcc gcggtgatcg
      421 cgtccggggt gctggcaggg ggagcagcag gcggcatgat ggtgtcgtcg ggcgtcacgt
      481 cagtctgttc aggcggttct tggcctggca tggccccgcc ttcaagccct atcggcaagt
      541 ctggacggat cgttgagccg ccagccccgc cttcgcgccg cttcacgtcc ccttcctccc
      601 gttaagcgca tggtccaccc gagccagggt ttcgtcgcgc ccgagcagcg cgactgtctc
      661 gaagataccc ggtgagacgg tcgttccagt cactgcgacg cgtaatggct gaaatacctg
      721 cttgcgcttg acacccagcc gctcggccac gtcacccagc gcggactcca ggttttcctc
      781 ggtccagggc accggaacgt ccgccagagc ggctcggacg gcgctcagcg cgtccttggc
      841 gccaggcgct tcgagcacct tctcgcgggc cgccggatcg tccgccgggc cgtcgaagaa
      901 gaactgcgtc aggggccaga actccgccag ggtggagatc ttgtcctggg cgatctcgac
      961 cgcaccgcgg agtccgctcc gaccggtcag atcctccagt cgagccgtca ggtcgtcgac
     1021 atccaactcg cggacgtagc gcccgttcat ccaacgcagc ttctgctcgt cgaacacggc
     1081 cggcgacttg gatacccgct ccaggcggaa acgctcctgc agctcctgga tcgtgtggaa
     1141 ggtcgtctcc tcgtcgtacc cccagccgag cagcgccaaa tagttgatga cggcctccgg
     1201 caggtagccc gcgtcgcgca gatcctgcac cgatgtggcg ccgtgccgct tgctcagctt
     1261 cttgccgtcc ggtccgtgca ggagcggcag gtggccgtag accggcgtcg gggcccctat
     1321 ggcgttctgg atgagcatgt gcttgggcgt gttcgagtag tggtcggcgc cgcgtacgac
     1381 gtgggtgatc ccggcgtcgg cgtcgtcgac caccaccgcc aggtggtaga ccgccgagcc
     1441 gtccgcacgg gcgatgacga gatcgtccag cagcgagttt tcgaacgcgc tctcgccccg
     1501 gatcacatct gtgaccactg tcactccctc gtccgggacg tggaggcgga cggcgccgct
     1561 cccctcgtcc tcaccgcgga agccacggtc gccgtatcgc tccttgaatg ccttgacctc
     1621 gtcgggcccg gcgctggtgc ggtaggcgtg cccgtcggcc agcagctgct cgacgaccgc
     1681 gcggtggcgg tcggcgttct gcgactggta ggtcggcccc tcgtcccagt cgatgccgag
     1741 ccactccagc gcgtcgaaga tcgtctggac gttctctggg gtcgaccgct cgcggtcggt
     1801 gtcctcgatg cggagcacga gctggccgcc ctggccacgg gccagaagcc agttgtagag
     1861 cgccgtccgc gcgttcc
//
LOCUS       DATBFZ010000418         9741 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_2911988, whole genome shotgun sequence.
ACCESSION   DATBFZ010000418 DATBFZ010000000
VERSION     DATBFZ010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 9741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9741
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2911988"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..677)
                     /locus_tag="VH419_12605"
     CDS             complement(<1..677)
                     /locus_tag="VH419_12605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020392746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HEY7044503.1"
                     /translation="MSSSGRLIRARVGAAALVAAAVATTSIGVPLGSTADAAPQQAAT
                     AHCTPSPDAPKRQFRAEWIASVTNIDWPSQPGLSVAQQKAELISWYDEAVDRGLNAVI
                     VQVRPTADAFWPSPIEPWSKYLTGTPGQDPGYDPLAFAVREAHARNLEFHAWFNPYRV
                     SMDTDRDALAPNSPAREHPDWVVEYGGKLYYNPGIPAARHLVEDAIMDAVEKYDIDAV
                     HFDDYFYP"
     gene            781..1377
                     /locus_tag="VH419_12610"
     CDS             781..1377
                     /locus_tag="VH419_12610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008360393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEY7044504.1"
                     /translation="MEFQQVVERRRMVRNYSDEPVDPVVVDRIMSNALHAPSAGFSQG
                     WGFLLLDTPADVARFWACTTDEGELPDSWLRGMHRAPVVVVALSCKDVYLDRYAEPDK
                     GWTDRDEHRWPVPFWDIDTGMAVLLMLQTVTDEGLGGCFFGVPREHWEAFRAEFGVPA
                     AYTPIGAVTIGHRASDTKSPSLKRGRKPVADVVHRSHW"
     gene            complement(1469..2911)
                     /locus_tag="VH419_12615"
     CDS             complement(1469..2911)
                     /locus_tag="VH419_12615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEY7044505.1"
                     /translation="MTSDTGLPTTALGWAAAFRDGTAHPTDAVEHYLTRTEKFDETVG
                     AFVRLTADRALEQAVEAGRRIDSGTPLSLLDGVPTGIKDLAATRGVETNFGSATLRGH
                     VPDFSDEVVRRIEAAGMISLGKTNAPEFGAPCYTEPDVAPPARTPYDLNRSAGGSSGG
                     AAAAVASGLLPVAHASDGGGSIRIPASVTGLVGFKPSRGRISSAPIYGDITGLSTSGP
                     LASTVRDGAAFLDVMAGPAMTDALWAADPPDSSTYLDWCERDPGRLSIARFSTPMIAE
                     VGVDTDVLAAYDDMSRRLADLGHELVDVPVPFSPELVPVFEVVWSVMTASWPLPAHQE
                     SGLRPLTRWLRDRGANTRAPDFSNALVRMRQAAAGALGALAAYDVVLTPTLAKPPALV
                     GAIRDDADPAQDFENQKAFTPFTAAWNITGMPAVSLPTGWTSSGLPIGTMLAAKPGQD
                     HVLFALAAQLEASIEAGPRGWSRPPLAIDG"
     gene            complement(3020..4696)
                     /gene="hutU"
                     /locus_tag="VH419_12620"
     CDS             complement(3020..4696)
                     /gene="hutU"
                     /locus_tag="VH419_12620"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012924320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HEY7044506.1"
                     /translation="MTETRTIRAPRGTELTARSWQTEAPMRMLMNNLDPEVAEHPEEL
                     VVYGGSGKAARDWPSYDAIVRSLTDLRQDETLLVQSGRPVGVMQTHEWAPRVLIANSN
                     LVGDWATWEEFRRLEQLGLTMYGQMTAGSWIYIGTQGILQGTYETFAAVAAKLASAGR
                     NEHVGGTLAGTITLTAGLGGMGGAQPLAVTMNDGVCICIECDESRIRRRIDHRYLDVQ
                     ASSLEEAVRLATEAKDGRKPLSIGVLGNAAEMMPELLAMGAPIDIVTDQTSAHDPLMY
                     LPVGVDFEDWVSFREKDPADFTARARESMARHCEAMVGFMDAGAEVFDYGNSLRAEAK
                     LGGFDRAFEYPGFVPAYIRPLFCEGKGPFRWAALSGDPKDIAATDRAILDLFPDDENL
                     RRWITKAGERVHFQGLPARICWLGYGERDRAGLRFNEMVASGELQAPLAIGRDHLDCG
                     SVASPYRETEAMLDGSDAIADWPLLNAMVNVASGASWVSIHHGGGVGIGRSIHAGQVV
                     VVDGTDLAAHKAQRVLTNDPGMGVIRHVDAGYERADEVARDRGVPIPMRS"
     gene            complement(4787..6331)
                     /gene="hutH"
                     /locus_tag="VH419_12625"
     CDS             complement(4787..6331)
                     /gene="hutH"
                     /locus_tag="VH419_12625"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013861528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HEY7044507.1"
                     /translation="MTSTVQVGVGPVSTDDVIAVARDRAQVELTDDARKALAAARSAV
                     DDLAAAPVPAYGISTGFGALATRHIPTSMRTQLQKSLIRSHAAGSGEPVEGEVVRALM
                     LLRLSTLATGRTGVRPETAETIAALLNHGITPVVHEYGSLGCSGDLAPLAHCALAVMG
                     EGDVFDADGTRRPAADALAAASIEPVELAAKEGLALINGTDGMLGMLVLAVHDLRRLL
                     TVADVGAAMSVEGLLGTDRVFAADLQELRPHPGQAASAANLRALLLDSEVVASHRGPD
                     CNRVQDAYSLRCSPQVVGAARDTVEYAAAVAGRELASAIDNPVVLEDGRVESNGNFHG
                     APVAYVLDFLAIPVADVASMAERRTDRFLDAARNSGLPPFLADDPGVDSGHMIAQYTQ
                     AAVVSELKRLANPASVDSIPSSAMQEDHVSMGWSSARKLRRGIDGLKRVLAIEILTAA
                     RGLDLRAPLRPAPATAAVIGALRRHVPGPGPDRHLAPDIEATVDLVDSGALVAAANSA
                     LPTPLL"
     gene            6422..7249
                     /locus_tag="VH419_12630"
     CDS             6422..7249
                     /locus_tag="VH419_12630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017935947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEY7044508.1"
                     /translation="MSQVQVPAATRALRVLRFLASQPEPVPLERIVHAVGLPRSSAYH
                     LVNAMIAEGFVTHVEDEHRYALGVAAFEVGSGYTRQAPLQRIARRPLAELVDSLGQSA
                     HLAVLHGRDVYYVLEERAAQKQVLVSDVGVRLPAHITASGRAILAFLAPAQVRALFPD
                     RDTFVTRHGTGPSSLTALTSVLAATRQRGYATEQDDVTPGFSSVAAPVLDHNGHPLAA
                     VAVTYAADDPPDVETTVGRVTATAAALSRRLGGGALRRRGDGGPEPSGGVTLRRPIG"
     gene            7325..>9741
                     /locus_tag="VH419_12635"
     CDS             7325..>9741
                     /locus_tag="VH419_12635"
                     /inference="COORDINATES: protein motif:HMM:NF014214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M36 family metallopeptidase"
                     /protein_id="HEY7044509.1"
                     /translation="MSQHVSSARRGAIAFLAATAVAGLTVSALPNGAVASAAHRDASS
                     ARTKTSSPAATSPTNARDSKDDFDARTVSEPGAVAVAEKAVKGTAPADVAKLRKQLGA
                     SAIVDIDPLTGTPANLTALNRFLTGRSSAPAGNVVMQYVRRHARELGLRSSDLSTFRL
                     NRSYVDPAGIRHLAWTQSVKGVTVFGNGLEAHVTKNGQLISLQGSPVSGLSQLTTGMS
                     SAPAMSASRAREASAKDVGATVAPGGERSLGKGEATLWSNGDRATPAWFVTPGGVRLA
                     WSTYTSSGDGLDFAHVLDASTGRVLYRHDLVDHDRGDAKVYDNYPGAAHGGTAHVVNF
                     VKRGWIGRGASWLTGANVEAWADLNDDDLVNAGEKTPLPGTKHGAQFTLQKFHSNPLC
                     SPRFVCTWNPNRARSWQTNKKADVANAFYLANTFHDYLAKRPIGFTAGAGNFEAADGD
                     PVLLNALDGANTANGLPDGAHIDNANMSTPPDGQAPTMQMYLWHFPHTTNAEEPFIPV
                     SGAFDASILFHEYTHGLSGRLVVDAQGNEALGSIQAGAMGEAWSDWYAQDYLVAKGFE
                     RDSVDKDGQILEGKYTLANQFPFRTMAMDCDPDSTAANCTDPITGDRGGYTYGDFPRI
                     GGSPEVHSSGEVWSQTLWDIREQYGHTVAAALVTRGMQLSPNDPTMLDMRNAIIQADK
                     VVYNSKHATGLWRIFANRGMGWYAGTTEGGDVTPAEDFHVPPPAHLGLGSIGGIVIDH
                     VTGDPIEGARVTITGHPGYTDTTDADGSYLIGNVKPGTYKKVVVSGSGYEVVVQQVRV
                     PGAGAAKR"
BASE COUNT         1573 a         3390 c         3311 g         1467 t
ORIGIN      
        1 gggtagaagt agtcgtcgaa gtggacggcg tcgatgtcgt acttctcgac ggcgtccatg
       61 atcgcgtcct cgacgaggtg ccgggcggcc gggatgccgg ggttgtagta gagcttgccg
      121 ccgtactcga cgacccagtc cgggtgttca cgcgccgggc tgttcggcgc cagtgcgtcc
      181 cggtcggtgt ccatcgagac gcggtagggg ttgaaccagg cgtggaactc gaggttgcgc
      241 gcgtgcgcct cgcgcaccgc gaaggcgagc gggtcgtagc ccgggtcctg accgggcgtg
      301 ccggtgaggt acttcgacca gggttcgatc ggcgacggcc agaacgcgtc agccgtcggc
      361 cggacctgca cgatgaccgc gttgaggcca cggtcgaccg cttcgtcgta ccagctgatg
      421 agctcggcct tctgctgggc cacggaaaga cccggctggg acggccagtc gatgttcgtg
      481 acgctggcga tccactccgc gcggaactga cgcttcggtg cgtccggcga tggcgtgcag
      541 tgggctgttg ccgcctgctg cggggcggcg tcggctgtgg atcccagcgg tacgccgatc
      601 gaggtggtgg cgacggccgc agcgacgagc gcggcggcgc cgacgcgggc ccggatgagt
      661 cggccggacg aggacatgac gatcactccc tggggtggca gacaagtgaa ccattggtct
      721 ggaccaatca accgttttcg tccgtacctg tcaacaggtc ggatgggaga ggatgtggcg
      781 atggagttcc agcaggtcgt cgagcgacgt cggatggtcc gcaactactc ggatgaaccc
      841 gtcgatcccg tggtcgtcga ccgcatcatg tcgaacgcgc tgcacgcccc gagcgccggg
      901 ttcagccagg gctggggatt cctgctgctc gacacgccgg ccgacgtcgc ccggttctgg
      961 gcctgcacga cagacgaggg cgagctgccg gacagctggc tgcgcgggat gcatcgcgca
     1021 cctgtcgtcg tggtggcgct gtcgtgcaag gacgtgtacc tcgaccggta cgccgagccg
     1081 gacaagggtt ggaccgaccg cgacgagcat cgctggccgg tgccgttctg ggacatcgac
     1141 accggtatgg cggtgttgct catgctccag accgtcaccg acgaggggct cggcgggtgc
     1201 ttcttcggcg taccgcgtga acactgggag gcgtttcggg cggagttcgg cgtgccggcg
     1261 gcgtacaccc cgatcggcgc ggtgaccatc ggccaccgcg cctctgacac gaagtcaccc
     1321 tccctgaaac gcggccgcaa acccgttgcc gacgtcgtcc accgctccca ctggtaaccc
     1381 cccccccgtt gagtgctggg ttcggggccg tcgagtgctg ggttcctggc cgtcgagtgc
     1441 tgggttcctg gccgtcggga ggtcaggact acccgtcgat cgcaagcggc ggccgagacc
     1501 acccgcgtgg accggcctcg atcgacgcct cgagctgcgc agccagcgca aacaggacat
     1561 ggtcctgtcc tggcttggcg gcgagcatgg tgccgatggg cagtccgctg ctcgtccatc
     1621 ccgtgggcaa cgagaccgcc ggcatgccag tgatgttcca ggcagccgtg aacggcgtga
     1681 acgccttctg gttctcgaag tcctgcgccg ggtcggcgtc gtctcggatg gcgccgacga
     1741 gcgccggtgg tttggccagc gtcggggtga ggaccacgtc gtacgccgcc agcgcaccga
     1801 gcgccccggc cgccgcttgc cgcatccgta ccaatgcgtt ggagaagtcg ggggcgcgtg
     1861 tgttggcgcc acggtcgcgc agccatcgag tcaacggtcg gagaccggac tcctggtggg
     1921 cgggcagtgg ccaggaggcg gtcatcaccg accacacgac ctcgaatacg gggaccagct
     1981 ccgggctgaa cggcacgggt acgtccacga gctcgtgccc gaggtcggca aggcggcgcg
     2041 acatgtcgtc gtacgccgcc aacacgtccg tgtcgacgcc gacctcggcg atcatcggcg
     2101 tactgaacct ggcaatcgac agccggccgg ggtcacgctc gcaccagtcg aggtacgtgc
     2161 tcgagtcagg tgggtcggcc gcccacagtg cgtcggtcat cgccggaccc gccatcacgt
     2221 cgagaaacgc cgcgccgtct cgcacggtcg aggcgagcgg gccggaggtg gagaggccgg
     2281 tgatgtcgcc gtagatcgga gccgagctga tccgcccacg gctcggcttg aacccgacca
     2341 gccctgtcac gctggccggg atgcggatcg acccgccacc atcggaggcg tgcgcgaccg
     2401 gcagcaggcc actcgccacc gccgccgcgg cgccgccaga cgagccaccg gcactgcggt
     2461 tcaagtcgta cggcgttcga gccggtggcg ccacgtccgg ctccgtgtag cagggtgcgc
     2521 cgaactccgg ggcattggtc ttgcccaagc tgatcatccc ggctgcctcg atccggcgta
     2581 cgacctcgtc ggagaaatca gggacgtggc cgcgcaacgt cgccgagccg aagttcgtct
     2641 cgacgccgcg ggttgcggcc aagtccttga tgccggtcgg tacgccgtcc agcagtgaca
     2701 gcggagtacc agagtcgatc cgcctgcctg cctctaccgc ctgctcgaga gctcgatctg
     2761 cggtgagccg gacgaacgcc ccgacggtct cgtcgaactt ctccgtcctc gtcaggtagt
     2821 gctcgacggc gtcggtcgga tgtgccgttc cgtcgcggaa agccgccgcc cagccgaggg
     2881 cggtggtcgg gaggccggta tcggaagtca tgcagccacg ctagccaggg gccgctgcgg
     2941 cactcgtcaa ccagcaactc cgcactcaac gaaccacaac tcaacgctca acgaaccaga
     3001 acccagcact caacgaagat cagctgcgca tggggatggg gacgccgcgg tcacgggcca
     3061 cctcgtcggc gcgctcgtaa cccgcgtcga catggcggat gacgcccata cccggatcgt
     3121 tcgtcaggac ccgttgcgcc ttgtgcgctg ccaagtccgt cccgtccacc acgacgacct
     3181 ggccggcgtg gatcgaacgg ccgattccga caccgccgcc atggtggatc gacacccagg
     3241 acgcgccgga cgccacgttg accatcgcgt tgagaagcgg ccagtcggca atcgcatccg
     3301 atccgtcgag catggcctcg gtctcgcggt acggcgacgc caccgacccg cagtccaggt
     3361 ggtcccggcc gatcgccaac ggcgcctgca gctcacctga ggcgaccatc tcgttgaacc
     3421 gcagcccagc acggtcgcgc tcgccgtatc ccagccagca gatgcgtgcc ggcagcccct
     3481 ggaagtggac gcgctccccc gccttcgtga tccaccgacg caggttctcg tcgtccggaa
     3541 acaggtccag gatcgcccgg tcggtcgcag cgatgtcctt cgggtcgccg gacaatgcag
     3601 cccaccggaa cggacccttg ccctcgcaga acaacggcct gatgtacgcc gggacgaagc
     3661 ccgggtactc gaacgcccga tcgaagccgc ccagcttcgc ctcggcgcgc agcgagttgc
     3721 cgtagtcgaa gacctcggca cccgcgtcca tgaaccccac catggcctcg cagtgacgcg
     3781 ccatggactc gcgcgcgcgt gcggtgaagt cggccgggtc cttctcgcgg aacgacaccc
     3841 agtcctcgaa gtcgacgccg accggcaggt acatcagcgg atcgtgggct gacgtctggt
     3901 cggtgacgat gtcgatcggc gcgcccatcg ccagcagctc cggcatcatc tcggcagcat
     3961 ttccgagaac gccgatggac aacggctttc gcccatcttt cgcctcggtg gcgagccgca
     4021 ccgcctcctc caacgaggac gcctggacgt cgaggtagcg atggtcgatc cggcgcctga
     4081 tccgggactc gtcacactcg atgcagatgc agacaccgtc gttcatcgtc accgccagag
     4141 gctgcgcacc acccatgccg ccgaggccgg ccgtcagcgt gatcgtgccg gcaagtgtgc
     4201 cgccgacgtg ctcgtttcgg ccggcgctcg cgagcttcgc cgccaccgcc gcgaacgtct
     4261 cgtacgttcc ctgcaggatt ccctgggtgc cgatgtagat ccacgaaccc gcggtcatct
     4321 ggccgtacat cgtgagcccg agctgctcga gccgccggaa ctcctcccag gtcgcccagt
     4381 cgccgaccag gttggagttg gcgatgagca cgcggggtgc ccactcgtgg gtctgcatca
     4441 cgccgacggg ccggccgctc tgcacgagca gcgtctcgtc ttggcgcagg tcggtcagcg
     4501 accggacgat cgcgtcgtac gacggccagt cacgcgccgc cttgcccgag ccgccgtaca
     4561 cgacgagctc ctcgggatgc tcggcgacct cagggtcgag gttgttcatc agcatccgca
     4621 tcggcgcctc ggtctgccac gaccgagccg tcagctcggt cccacggggg gcccggatcg
     4681 ttcgtgtctc agtcatcgca agtcctttcc aacggcgtcg atcgaggtgg cagtcgcggt
     4741 ccgacacgcc gtcaaaacgc aacgaaagtt gccacctcgg cgggggtcac aggagggggg
     4801 tggggagggc ggagttggcg gcggcgacga gggcgccgga gtcgaccagg tcgacggtgg
     4861 cctcgatgtc gggtgcgagg tgccggtccg gacccggacc gggtacgtgt cggcgcagcg
     4921 cgccgatcac cgctgcggtg gccggcgccg gtcgtagcgg agcacgcagg tcgagcccgc
     4981 gagcggcggt caaaatctcg atcgccagca cgcgcttgag cccgtcgatt ccgcggcgta
     5041 gcttgcgcgc cgacgaccag cccatcgaca cgtggtcctc ctgcatcgcg ctggacggga
     5101 tggagtcgac gctggccggg ttggcgagcc gcttcagctc cgacaccacg gctgcctggg
     5161 tgtactgggc gatcatgtgt ccgctgtcaa cgcccgggtc gtcggcgagg aacggcggca
     5221 ggccggagtt gcgggccgca tcgaggaacc gatccgtacg ccgttcggcc atgctcgcca
     5281 catccgcgac cgggatcgcc aggaagtcga gcacgtacgc caccggggca ccgtggaagt
     5341 tcccgttgct ctccacccga ccgtcctcga gcacgaccgg gttgtcgatc gccgaggcga
     5401 gctcccgtcc ggccacggcg gcggcgtact cgaccgtgtc ccgcgccgca ccgacgacct
     5461 gcggcgaaca gcgcaacgaa taggcgtcct gcacgcggtt gcagtccggg ccgcggtggc
     5521 tggcgaccac ctcggagtca agcaacaacg cacgaaggtt ggcagccgac gcagcctggc
     5581 caggatgtgg acgaagctcc tggagatcgg cggcgaacac gcggtcggtg ccgagcagcc
     5641 cttcgaccga catggcagca ccgacgtccg ccacggtcag caacctgcgg aggtcgtgga
     5701 ccgccagcac gagcatgccg agcatgccgt cggtcccgtt gatcagtgcc agaccttcct
     5761 tcgcggccag ctcgaccggt tcgatggacg ccgccgcgag cgcatcggcg gccggtcgcc
     5821 gtgtcccgtc ggcgtcgaac acgtcaccct cacccatcac ggccagcgcg cagtgtgcga
     5881 gcggcgccag gtcgcccgag caaccgaggc tgccgtactc gtgcacgacc ggagtgatgc
     5941 cgtggttgag cagcgcggcg atcgtctcgg cggtctcggg gcgcaccccg gtccgcccgg
     6001 tggccaacgt cgagagccgc agcagcatca gggcgcgcac cacctccccc tcgacgggtt
     6061 cgcccgatcc tgcggcgtgg gaccggatca gggacttctg cagctgggtc cgcatcgacg
     6121 tcgggatgtg ccgcgtggcg agggcgccga aaccggtgga gatgccgtac gccggtacgg
     6181 gtgcggcagc gaggtcgtcc accgcgctgc gtgctgcggc aagcgccttg cgcgcatcgt
     6241 ccgtcagctc gacctgggca cggtcgcgtg caacggcgat gacgtcatct gtggacacgg
     6301 gtccgacgcc gacctgcact gtggatgtca tgcagccatt ccactgcgct ggaccggcgg
     6361 cgaccagacc tacggcacga gcagagtctg agatccgaga caccgctcta gactgatgga
     6421 gatgtcccag gtccaggttc cggcggcgac ccgcgcgctc cgggtgttgc gtttcctcgc
     6481 ctcccaaccc gagccggtgc cgctcgaacg gatcgttcac gccgtcggcc tgccgcgctc
     6541 gtcggcgtac cacctggtca acgcgatgat cgccgaaggg ttcgtgacgc acgtcgagga
     6601 cgagcaccgg tacgcgttgg gggtggcggc gttcgaggtc ggcagcgggt acacccggca
     6661 ggcaccgttg cagcggatcg cccgccgacc gctggcggag ctcgtggact cgctgggcca
     6721 gtccgcgcac ctggccgtgc tgcacggtcg ggacgtgtac tacgtcctcg aggaacgggc
     6781 cgcccagaag caggtgctgg tcagcgacgt cggagtccgg ctgccggccc acatcaccgc
     6841 gagcggccgc gcgatcctgg ccttcctcgc cccggcacag gtacgcgcgc tgttcccgga
     6901 ccgcgacacc ttcgtcacgc gccacgggac cggtccgagc agcctgactg cactgacgtc
     6961 agtgttggcg gcgacccgtc agcgcggcta cgcgaccgag caggacgacg tgacgccggg
     7021 cttctcctcg gtggccgcgc cggtgctcga ccacaacgga catccgttgg cagcggtggc
     7081 tgtgacgtat gccgccgacg acccgccgga cgtcgagacg accgtcgggc gagtcaccgc
     7141 caccgccgcg gcactgtcgc gacgactggg cggaggcgcc ctgcgccgtc gcggtgacgg
     7201 cggccccgag ccatcaggtg gtgtgacgtt gcgacgaccc atcgggtgaa gagcgtcaca
     7261 aggtgactta gggtgtcccc cgcatgccat ctgcagcttc cctacctctc ttggaggacc
     7321 cctcgtgtca caacacgtca gtagcgcccg acgtggtgcg atcgcgttcc tcgcggcaac
     7381 cgccgtcgca ggactcacgg tcagcgcact accgaacggt gccgtggcca gcgccgcgca
     7441 ccgagatgca tccagcgccc ggacgaagac ctcctctccg gccgcaacct cccccacgaa
     7501 tgcccgcgac agcaaggacg acttcgacgc caggacggtc tcggagcccg gcgccgtcgc
     7561 cgtcgccgag aaagcggtca aaggtacggc tcctgccgac gtcgccaagc tgcgcaagca
     7621 gctgggagca tctgcgatcg ttgacatcga cccgctcacc gggacgccgg ccaatctgac
     7681 ggctctcaac cgcttcctga ccgggcgcag cagcgcgccc gcagggaacg tcgtgatgca
     7741 gtacgtgcgc cggcacgcgc gcgagctggg tctgcgcagt tccgacctga gcacgttccg
     7801 gttgaaccgt tcgtacgtcg accccgccgg catcaggcac ctggcctgga cgcagtccgt
     7861 caaaggcgtc acggtcttcg gcaacggtct cgaagcccac gtgacgaaga acggccagct
     7921 catctccctc caggggtcgc ctgtctcggg gctttcccag ctcaccacag gcatgtcgtc
     7981 cgcacccgcc atgtccgcga gccgcgcccg ggaggcgtcc gcgaaggacg tcggcgcgac
     8041 cgtcgcacct ggtggcgaac gctcgctcgg caagggcgag gcgacgctct ggtccaacgg
     8101 tgacagggcc acgccggcct ggttcgtgac acctggtggt gtccggctgg cgtggtcgac
     8161 gtacacgagt tctggcgacg gtctggactt cgcgcacgtg ctcgacgcca gtaccggccg
     8221 cgtcctctac cgacatgacc tcgtcgacca cgaccgtggc gacgccaagg tctacgacaa
     8281 ctacccgggt gccgcccacg gcggcacggc ccacgtggtc aacttcgtga agcgaggctg
     8341 gatcggcagg ggcgccagct ggctcaccgg cgccaacgtc gaagcgtggg ccgacctgaa
     8401 cgacgacgac ctcgtcaatg ccggcgagaa gaccccgctg cccggcacca agcacggcgc
     8461 gcagttcacg ctgcagaagt tccacagcaa cccgctgtgc agcccgcggt tcgtgtgcac
     8521 ctggaacccg aacagggcgc gctcgtggca gaccaacaag aaggccgacg tggccaacgc
     8581 cttctacctc gccaacacat tccacgacta cctggcgaag cggccgatcg gcttcacggc
     8641 cggcgccggc aacttcgagg ccgctgacgg tgaccccgtg ctcctcaacg cgctcgacgg
     8701 cgccaacacc gccaacggcc tgcccgacgg tgcccacatc gacaacgcga acatgtcgac
     8761 gcctccggac ggccaggcgc cgaccatgca gatgtacctg tggcacttcc cgcacaccac
     8821 caacgcggag gagccgttca tcccggtgag cggggccttc gacgcgagca tcctgttcca
     8881 cgagtacacc cacggcctct cgggccgtct ggtcgtggat gcgcagggaa acgaggcgct
     8941 gggttccatc caggccggcg ccatgggcga ggcgtggagt gactggtacg cccaggacta
     9001 cctcgtcgcg aagggcttcg agcgagacag cgtcgacaag gacggccaga tcctcgaggg
     9061 caagtacacg ctggcgaacc agttcccgtt ccggaccatg gcgatggact gcgacccgga
     9121 ctccacggcg gcaaactgca ccgacccgat cacgggcgat cgcggtggct acacctacgg
     9181 tgacttccct cgtatcggtg ggtcacccga ggtccactca agcggtgagg tctggtcgca
     9241 gacgctgtgg gacatccgag agcagtacgg ccacacggtc gccgctgcgt tggtgacccg
     9301 gggcatgcag ctgtctccga acgacccgac catgctggac atgcgcaacg cgatcatcca
     9361 ggccgacaag gtcgtctaca acagcaagca cgcgacgggc ctctggcgga tcttcgccaa
     9421 ccgcggtatg ggctggtacg ccggaacgac cgagggcggt gacgtgacac cggctgagga
     9481 cttccacgtg ccaccgccgg cgcatctcgg tctcggctcc atcggcggca tcgtcatcga
     9541 ccacgtgacg ggcgacccga tcgagggcgc gcgggtgacg atcaccggac acccgggcta
     9601 caccgacacc accgatgccg acggctccta cctgatcggc aacgtcaagc cgggcacgta
     9661 caagaaggtc gtcgtgtccg gttccggtta cgaggtcgtg gtgcagcagg tccgcgttcc
     9721 gggcgccggc gcggccaagc g
//
LOCUS       DATBFZ010000419         8926 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_5071678, whole genome shotgun sequence.
ACCESSION   DATBFZ010000419 DATBFZ010000000
VERSION     DATBFZ010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 8926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8926
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5071678"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..457)
                     /locus_tag="VH419_12640"
     CDS             complement(<1..457)
                     /locus_tag="VH419_12640"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY7044510.1"
                     /translation="MTKPTVALRGVGKSFSGSPALSDVDLDLHRGVVGLLGPNGAGKT
                     TLLRILATVLAADSGDVDILDRDPADGVQRTDIRRRLGYLPQELGYPRGFTAYGFVDY
                     IAVLKEWDDAARRRAEVRTVLEHVGLADRATKRIRALSGGQRRRVGLAQA"
     gene            complement(483..1325)
                     /locus_tag="VH419_12645"
     CDS             complement(483..1325)
                     /locus_tag="VH419_12645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044511.1"
                     /translation="MNAPDQQPWHVDDVSLRRWVEGTSGAVASVSVEEHVLRCERCQA
                     EVADLVPTAPLQPVWDNVLAGIEIPHAGLAERLLRRLGLRASDALVIGSAVALRAAWL
                     SAVIAVLFFTVVAGVMGRDGGLVLFLMAAPLIPISGVAAAYGPSADPSYDITLAAPYA
                     MVRLVLLRTAYVLVTSVPLVVVAGLLLPTTSSVVAVAWLLPAAGFVVVVLSASNWVDP
                     AYAAATVAVGWIITVAAVVARSRDPLEVFAPIALVTYLAIIVVAGLILLQRLFSAVPS
                     WRLR"
     gene            complement(1322..1873)
                     /locus_tag="VH419_12650"
     CDS             complement(1322..1873)
                     /locus_tag="VH419_12650"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY7044512.1"
                     /translation="MEEAADRSDGELMRAVVDGDTAALRALYERHSPWMSVRLVRRCN
                     DASVVSEVLQDTFVAVWRGAAGFRGEGDVGGWLWGIAIRRLVSRLRKHDRRAEVLVAD
                     VGGGAERSAEELVLTGVEYGDLGLAMSRLSPQMRAVVQATVLDGLTTREAAQLLGVPQ
                     GTVKTRLHRAKAQLRQELTGGLA"
     gene            2101..3183
                     /locus_tag="VH419_12655"
     CDS             2101..3183
                     /locus_tag="VH419_12655"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF008277.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HEY7044513.1"
                     /translation="MTDSRVTKARTEPFGLTGSGQPVERIRLRSEQVQVDVITYGARV
                     TSVLAPDQDGVLDEVILGLADVAAYEADRNYLGATIGRYANRIADGRFELDGHRFDIP
                     ANEGSNALHGGTRGFDSMLWEAEVGEGAEVRLSRVSDDGEMGFPGRLEVTVTYRLDGP
                     DLRVSYDARTSAPTVVNLTNHMYVNLAGGGSVEDHLIQVDAEAFLPIDDHAIPTGEVR
                     RVEGTPFDLRSPVRIGDRLRAADEQLRRALGYDHTFVLGPVSDEETISAERQMRRAAR
                     LEEPASGRGLELWTDRPGVQVYTGNHLDGTTIGRDGTTYRQTDACCLEPQHFPDAPNH
                     PAFPSTVLRPGDVYRARDVYRFTTDR"
     gene            3322..4713
                     /locus_tag="VH419_12660"
     CDS             3322..4713
                     /locus_tag="VH419_12660"
                     /EC_number="1.8.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020657554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mycothione reductase"
                     /protein_id="HEY7044514.1"
                     /translation="MRHHDLVIVGTGSGNTIANRQLRHLDIGIVEHSTFGGTCINVGC
                     IPSKMFVHTADLATSTKDSGRFGIDAHVDKVRWIDIRDRIFGRIDPTTEAARRYREEG
                     ANTTFYAEHAEFVGRRRLRLASGEDIDAERVVVAAGSRPVIPEIEGFDRVSKHLVHTS
                     DTIMRIDDVPESMVILGGGTIAAEMAHVFSALGTRVSVIARSARLLRVEDDAVSKAFT
                     SIASSRWEVQTSRLAHRIAETGSGVRVHLVDALGRVDPRPVDAELLLVATGRTSNADQ
                     LNVDAAGIVTHEDGRIVVDEFQRTTADGVFALGDVSSDWQLKHVANHEARIVRHNLLH
                     PDDMIKVDHRFVPHAVFTHPQVASVGKTERDLVDSNTAYLSSTREYADVAYGWAMEDN
                     TGFVKVLADPDSGLLLGAHLIGPESSSLIQPLIQAMSFGLPVDQMARGQYWIHPALAE
                     AVENALLGLRFRH"
     gene            4881..6362
                     /locus_tag="VH419_12665"
     CDS             4881..6362
                     /locus_tag="VH419_12665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY7044515.1"
                     /translation="MSTGVETGRITTDIPARMDRLPWSRWHWIIVIGLGTVWILDGLE
                     VTIVGSLSDVLKSPTDGLGLSSSQVANAGSVYVAGACIGALFFGQLTDRFGRKKLFMI
                     TLGLYTLATAATAFSMNWEWYFAARFLTGAGIGGEYAAINSAIDELIPARYRGRIDIA
                     INGSFWVGAAAGALLTIPLLDPTIVTNQEIGWRLAFGLGVVLALLVLLVRQSVPESPR
                     WLFIHGREDDAEEIVEDIEHKVEEQTGEELPSVSDTITIRQRKTIGMGLIAKTVFTLY
                     PRRTVLCFSLFVGQAFLYNAFFFTYGDALSTFLGVTQTGWYLAIFAVCNFFGALILSP
                     LFDVVGRVAMIAGTYIISGVLLGVTGYILGDLSANTLTLMGGIVFFFASAGASAAYLT
                     ASEVFPMETRALCIAFFYAIGTAAGGITGPYLFGKLIENADTISDIAPGYFLGAALMV
                     IGGVVELFLGVKAEGQTLENIAKPLTAEDKPSDESKQTEPATA"
     gene            6425..7195
                     /locus_tag="VH419_12670"
     CDS             6425..7195
                     /locus_tag="VH419_12670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012918902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY7044516.1"
                     /translation="MEIVYDDNGSGPEVLLLHSGVCDRRMWRPQSEVLSRSHRVVAPD
                     LPGFGDSPLEPGEFSSTAELVRLLDNLDIRQVAVVGSSYGGRVALELVEEAPDRIRAL
                     VLLCPAYRGLDATATAETFDADEEGRLEAGDVDGAVELNVRTWLGPEADDATRDLVRT
                     MQRRAFDVQLAADAWPEPPEPIRVDPDLSAIGVPVFVVTGGHDMDHFQNIARHLAATI
                     PDAHLVELPWAGHLPSLERPAEVTALLTGFLAEPATGR"
     gene            complement(7214..8182)
                     /locus_tag="VH419_12675"
     CDS             complement(7214..8182)
                     /locus_tag="VH419_12675"
                     /inference="COORDINATES: protein motif:HMM:NF014601.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase N-terminal
                     domain-containing protein"
                     /protein_id="HEY7044517.1"
                     /translation="MRYVVYGAGAVGGVVGALLHRAGHDVVLIARGDHLAAIRRNGLR
                     LLTPAEDTTQHIPAAGDPAEIDWRHDDVVLLAMKSDGTRDAAVALAAAAPPSVAVVSV
                     QNGVANEPVLLRWFDCVYGVCVMAPTAHREPGVVEAHCHPTAQILDVGRYPHGVDVTA
                     EQVATAFESAGVVSVPRPDIMAWKYRKLLHNLGNAVQAVCRPDDDRQQLLGLVRDEGE
                     RVLAATGIHVVSEEADRRRRGDILQLAEIPGRPRRGSSSWQSLERRTGSIEADYLNGE
                     IVWLGRQFDVAAPANELVRREAIRHAAARRPPGTVPARELLDRLST"
     gene            complement(8249..>8926)
                     /locus_tag="VH419_12680"
     CDS             complement(8249..>8926)
                     /locus_tag="VH419_12680"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY7044518.1"
                     /translation="DALAVVSVLRAAGVVVALGHSDADHATFAAALAALEGSALVTHL
                     ANGMPALHHRAPGPVGAALTAAARGEATVELIADGVHLDDAFVELVFATASPGHVALV
                     TDAMAAAGVSDGDYELGPQRVAVRDGVARLAPPDEGDPDTLTEADATAAIAGGTAHLL
                     DVVRRTAATPVGLVEAVRAASSTPARVLGLDAQIGSLSSGKRADVLVVDEALQLQRVM
                     VGGSWQR"
BASE COUNT         1456 a         3117 c         2988 g         1365 t
ORIGIN      
        1 gcgcctgcgc gagaccgacg cgacgacgct gtcccccgga caaggctcgg atgcgcttgg
       61 tggcccgatc ggccagcccg acgtgctcga gcaccgtgcg aacctcagca cgacggcgcg
      121 cggcgtcgtc ccactccttg agcaccgcga tgtagtcgac gaagccgtac gccgtgaaac
      181 cgcgcggata cccgagctcc tgcggaaggt agcccagccg gcgccgaatg tcggtccgct
      241 gcaccccgtc agccgggtcg cggtcgagga tgtcgacgtc gccgctgtcc gcagccagca
      301 cggtggcaag gatgcggagc aacgtcgtct tgccggcgcc gttcgggccg agcagaccga
      361 cgaccccccg atgcagatcc aggtcgacgt ccgacagtgc cggagagccg ctgaagctct
      421 tcccgacgcc gcgcaacgcg acggttggtt tggtcatgat tcccccttgt gttcgagcac
      481 catcagcgca gccgccacga cggcacggca ctgaagagac gttggagcag gatcaggccc
      541 gcgaccacga tgatcgcgag gtacgtgacg agagcgatcg gtgcgaacac ctcgagcggg
      601 tctctgctcc gggcgaccac cgccgcaacg gtgatgatcc agccgacggc gaccgttgcg
      661 gccgcgtacg ccgggtcgac ccagttgctt gcactcagta cgacgacgac gaaacccgcc
      721 gccggtagca accacgccac tgcgacgacg ctcgaggtcg tcgggagcag cagtccggcg
      781 acaacgacga gcggcacgga cgtcacgagc acgtaggcag tacggagcag gacgagccgc
      841 accatggcat acggagcggc aagcgtgatg tcgtacgacg gatctgcgga cggaccatac
      901 gcggcggcca ctcccgagat cgggatcaac ggagccgcca tgaggaacaa gaccagaccc
      961 ccgtcacgac ccatgactcc cgcgaccacg gtgaagaaga gcaccgcgat caccgctgac
     1021 aaccaagccg cgcgcagcgc caccgccgat ccgatgacca acgcatcgga tgcgcgcagg
     1081 ccaagccggc gcagcagccg ttcggccaga ccggcgtgcg ggatctcgat ccctgccagc
     1141 acgttgtccc acaccggctg gaggggtgcg gtcgggacca ggtcggccac ctccgcctgg
     1201 caccgttcac agcgcagcac atgctcttcg acagagacgc tggccacggc gccggaggta
     1261 ccttccaccc atcgacgaag cgacacgtcg tcgacgtgcc acggctgttg gtcgggtgcg
     1321 ttcatgccag ccctcctgtc agctcctgcc gcagctgtgc cttggcccga tgcagtcgtg
     1381 tcttgacggt gccttggggt acgccgagca actgtgctgc ctcacgcgtc gtcaacccgt
     1441 cgagcacggt ggcctgcacg accgcccgca tctgcggcga gaggcgggac atcgccaggc
     1501 ccaggtcgcc gtactcgacg ccggtgagga cgagctcttc ggcactgcgt tcggcaccgc
     1561 cgccgacgtc tgcgaccagc acctcggcgc gtcgatcgtg cttgcgcaac cgggagacga
     1621 gtcggcggat ggctatcccc cagagccacc cgccgacgtc gccttctccc cggaacccgg
     1681 cggcgccacg ccagacggcg acgaacgtgt cctggagaac ctccgagacg accgatgcgt
     1741 cgttgcagcg acgtacgagt cgcaccgaca tccacggcga gtggcgctcg tagagcgcgc
     1801 gcaaggcagc cgtgtcgcca tcgacgacgg cacgcatcag ctcgccatcg gaccggtcgg
     1861 cggcttcctc cataccactc ctgtcgcagc cggacacctg gatggttcca cctcgctccg
     1921 gatgcacgcg ctccgacgag actcggccat cggctgcacc accaaggttc tcacccgttc
     1981 aggtggttgc gccgctgcga atgagcgccg cacgatcagg tgagcaagac caaggcggcg
     2041 tcggacttct tctccgctga gttgatgcga tcacacacct gaccgcggtt ggatcagaca
     2101 atgaccgatt cccgtgtcac gaaagcccgc actgagccgt tcggcctgac gggttccggc
     2161 caaccggtcg agcggattcg gctgcgttcg gagcaggtgc aagtcgacgt catcacgtac
     2221 ggcgcccggg tcacgtcggt gctcgcgccg gaccaggacg gcgtactcga tgaggtcatt
     2281 ctcgggctgg cggacgtggc ggcgtacgag gcggaccgga actacctcgg cgcgaccatc
     2341 ggacggtacg ccaaccggat cgcagacggc cggttcgagc tcgacggcca caggttcgac
     2401 attcctgcca acgagggcag caacgccctg cacggaggca cccgcggctt cgacagcatg
     2461 ctgtgggagg ccgaggtggg cgagggggcc gaagtccgac tgtcgcgagt cagcgacgac
     2521 ggcgagatgg gcttccccgg ccgtctggag gtcaccgtga cctaccgcct cgacggcccg
     2581 gatctacgcg tctcgtacga cgcccggacg tcggcaccga ccgtggtcaa cctgacgaac
     2641 cacatgtacg tcaacctcgc cggcggcggt agcgtcgagg accacctgat ccaggtcgac
     2701 gccgaggcct tcctgcccat cgacgaccac gcgatcccca ccggcgaggt ccggcgggtg
     2761 gagggcacgc cattcgacct gcggtcgccg gtgcgcatcg gcgaccgact gcgcgccgcc
     2821 gacgagcagc tgcgacgtgc gctcggctac gaccacacgt tcgtgctcgg ccctgtgtcg
     2881 gatgaggaga cgatcagtgc cgaacgacag atgcggcgcg ccgctcgact ggaggagcct
     2941 gcgtccggac gtggcctcga gctgtggacg gatcgacccg gcgtccaggt ctacacaggg
     3001 aaccatctcg acggcaccac catcgggcgg gacggcacga cgtaccggca gaccgacgcc
     3061 tgctgcctgg aaccccagca cttccccgat gcgccgaacc acccggcgtt cccgtcgacc
     3121 gtgcttcgac ccggtgacgt ctaccgcgcc cgcgacgtct accgcttcac caccgaccgc
     3181 tgagcttccc gcgaattgcg ggaggttccg cgatttcctg gctcgaaatc gcggagcttc
     3241 ccgcatttcc gggggcggcg ggcgcggccg gtcgccggga cggtgggtgt ggagtcgtcg
     3301 atgcggggca cgatagaacg tatgcggcac cacgacctgg tcatcgtcgg caccggatcg
     3361 ggcaacacga tcgcgaaccg ccaactgcgc catctcgaca tcgggatcgt cgagcactcg
     3421 acgttcggcg gcacctgcat caacgtcgga tgcatccctt cgaagatgtt cgtgcacacc
     3481 gcagacctgg cgacgagcac gaaggacagc ggtcggttcg gcatcgacgc gcacgtcgac
     3541 aaggtgcggt ggatcgacat ccgcgaccgc atcttcggac gcatcgaccc gaccaccgag
     3601 gctgcccggc ggtatcgaga ggagggcgcc aacacgacgt tctacgccga gcacgccgag
     3661 ttcgtcggac gtcggcgact gcgtctcgcg tccggcgagg acatcgacgc cgagcgtgtc
     3721 gtggtggccg cagggagccg gccggtgatc ccggagatcg aagggttcga ccgtgtcagc
     3781 aagcacctgg tgcacacatc ggacaccatc atgcgcatcg acgacgtacc cgagtcgatg
     3841 gtcatcctgg gcggtggcac catcgccgct gagatggcac acgtgttctc ggcgctcggc
     3901 acccgcgtca gtgtcatcgc gcggtcggcg cggctgctcc gggtcgagga cgacgccgtc
     3961 tccaaggcgt tcacgagcat cgcgtcgagt cggtgggagg tgcagacgtc acggctcgcg
     4021 caccggatcg cggaaaccgg atccggtgta cgcgtgcacc tggtcgatgc gctcggtcgg
     4081 gtcgaccccc gtcctgtcga cgccgaactg ctcctcgtcg ccaccggacg cacctccaac
     4141 gccgaccagc tgaacgtcga cgccgccggc atcgtcacgc acgaggacgg ccgcatcgtc
     4201 gtcgacgagt tccagcggac caccgccgac ggcgtcttcg cgctgggaga cgtgtccagc
     4261 gactggcagc tcaaacacgt cgccaaccac gaggcacgca tcgtccggca caacctgctg
     4321 caccctgacg acatgatcaa ggtggatcac cggttcgtgc cgcacgcggt cttcacccat
     4381 ccccaggtcg catcggtcgg aaagaccgag cgtgatctcg tcgacagcaa cacggcgtac
     4441 ctctcctcga cgcgggagta cgccgacgtc gcctacggct gggccatgga ggacaacacc
     4501 ggcttcgtga aggtgctggc ggacccggac tccgggctgc tgctcggtgc ccacctgatc
     4561 ggtccggagt cgtcgagcct gatccagccc ctcatccagg cgatgtcgtt cggcctgccg
     4621 gtggatcaga tggcccgcgg gcagtactgg atccatccag cactcgccga ggccgtggag
     4681 aacgccctcc tcggtctgcg tttccggcac tgacgtcgct cgactgcaca gcgcagttgc
     4741 gctcattgct ggacagcgca tttgcgctca ttcgacgcga agccgcctcc ctgtctgaat
     4801 gacgcgaact gaatgacgcg aaggggcatg ggtacctccc aggaggcgta cgacgccgta
     4861 cgcgcgtgaa cggaggagtc atgtcgacgg gagtcgaaac cgggcgcatc accaccgaca
     4921 ttccggcccg aatggaccgg ctgccgtggt cgcgctggca ctggatcatc gtcatcggac
     4981 tggggaccgt ctggatcctc gacgggctcg aggtcacgat cgtcggctcg ctgagcgacg
     5041 tgctgaagtc cccgaccgac ggactcggtc tgagcagctc gcaggttgcg aacgcgggct
     5101 cggtgtacgt cgcgggtgcc tgcatcggcg cgttgttctt cggtcagctg accgaccggt
     5161 tcggtcggaa gaagctgttc atgatcaccc tcgggctcta cacgttggcg acggcggcaa
     5221 cggcgttctc gatgaactgg gagtggtact tcgcggcccg gttcctcacc ggcgccggca
     5281 tcggtggtga gtacgccgcg atcaactccg cgatcgacga gctgatcccg gcccgctacc
     5341 gcggccgcat cgacatcgcg atcaacggtt cgttctgggt aggtgcggca gcgggagcgc
     5401 tgctgacgat cccgctgctc gacccgacga tcgtgacgaa ccaggagatc ggctggcggc
     5461 tggcgttcgg cttgggcgtc gtactggcgc tgctggtcct gctcgtccgg cagagcgtcc
     5521 ccgaaagccc gcgctggctg ttcatccacg gccgcgagga cgacgccgag gagatcgtcg
     5581 aggacatcga gcacaaggtc gaggagcaga cgggcgagga gctgccgagt gtctccgaca
     5641 cgatcaccat ccggcagcgc aagacgatcg gcatgggcct catcgcgaag accgtgttca
     5701 cgctctaccc gagacggacc gtcctctgct tctcgctgtt cgtcggacag gcattcctct
     5761 acaacgcgtt cttcttcacc tacggcgacg ccctcagcac gttcctcggc gtcacccaga
     5821 ccggctggta cctggccatc ttcgcggtct gcaacttctt cggcgcgctg atcctcagcc
     5881 cgctgttcga cgtggtcggc cgagtcgcga tgatcgccgg gacgtacatc atctccggtg
     5941 tcctgctcgg cgtcaccggc tacatcctcg gcgacctgag cgccaacacg ctcacgctga
     6001 tgggcggcat cgtcttcttc ttcgcctcgg ccggcgccag tgcggcgtac ctgaccgcga
     6061 gcgaggtgtt cccgatggag acccgggcat tgtgcatcgc gttcttctac gcgatcggca
     6121 cggcagccgg cggcatcacc gggccgtacc tcttcggcaa gctgatcgag aacgccgaca
     6181 cgatctccga catcgcccct gggtacttcc tgggcgctgc cttgatggtc atcggcggtg
     6241 tggtcgagtt gttcctcggt gtcaaggccg agggccagac cttggagaac atcgccaagc
     6301 cgctcaccgc tgaggacaag ccgagcgacg agagcaagca gacggagccg gcgacggcgt
     6361 gagccagcac tgcgacggtg caaaccattc gccccgccga tgcgggtgac cgtatggtcg
     6421 ccacgtggag atcgtgtacg acgacaacgg ttccggcccg gaagtcctgc tgctgcactc
     6481 cggtgtctgc gaccgccgca tgtggcggcc gcagtcggag gtcctctccc ggagccaccg
     6541 cgtggtggcc cccgacctgc cgggtttcgg ggactcgccg ctcgagcccg gcgagttctc
     6601 gtccaccgct gagctcgtcc ggctgctcga caacctggac atccggcagg tcgcggtggt
     6661 cgggtcgtcg tacggaggcc gcgtcgccct cgagctcgtt gaagaggcgc ccgaccggat
     6721 ccgtgccctc gtcctgctct gcccggcgta ccgcggcctc gacgccacgg cgaccgccga
     6781 gaccttcgat gccgacgagg aggggcgtct cgaggccgga gacgtcgacg gggcggtcga
     6841 gctgaacgtc cgaacctggt tgggtccgga ggccgacgac gcgacccggg acctggttcg
     6901 gacgatgcaa cgacgcgcgt tcgacgtaca gctggccgcg gacgcctggc ccgagccacc
     6961 tgaacccatt cgcgtcgacc ctgacctgag cgccatcggt gtcccggtgt tcgtggtcac
     7021 cggcggtcac gacatggacc acttccagaa catcgcccgg catctcgccg ccacgatccc
     7081 cgacgcgcac ctggtcgagc ttccgtgggc cggtcacctg ccgagcctcg agcgaccggc
     7141 cgaggtgacc gcgctgctga ccgggttcct cgccgagccc gccaccggtc gttgaccgcc
     7201 cggcggtccg gcgtcaggtc gagaggcggt ccagcagttc gcgggcgggg acggtgcctg
     7261 gcggccttcg cgcggcggcg tggcggatgg cctcccggcg caccagttca ttcgcgggtg
     7321 cagcgacgtc gaactgccgc cccagccaga cgatctcgcc gttgaggtag tcggcctcga
     7381 tggatccggt acgccgttcg agggactgcc acgacgagct gccccgtctt gggcgtccgg
     7441 ggatttcggc cagctgcagg atgtcaccac gtcgccgacg gtcggcctcc tcgctgacga
     7501 catggatgcc cgtggcggcc agtacgcgct ccccttcgtc cctgaccaga ccgagcagct
     7561 gctgccgatc gtcgtctggt cggcagactg cctgcacggc gttgccgagg ttgtgcagca
     7621 gcttgcggta cttccaggcc atgatgtccg gccgcggcac cgacacgacc ccggccgact
     7681 cgaacgctgt cgccacctgc tcggcggtca cgtcgacgcc gtggggatac cggccgacgt
     7741 cgaggatctg ggccgttgga tggcagtgcg cctcgacgac accgggttcg cgatgcgcgg
     7801 tcggggccat cacgcacacg ccgtacacgc agtcgaacca gcgcagcagg accggttcgt
     7861 tggccacgcc gttctgcacg gacacgaccg caacactggg tggtgcggcc gcggccagcg
     7921 cgacggcggc gtcgcgggtc ccgtcgctct tcatcgccaa aagaacgacg tcgtcgtgac
     7981 gccagtcgat ctcagccggg tcgccggcag cgggaatgtg ctgggtcgtg tcctccgcgg
     8041 gggtcagcag ccgcagcccg ttccggcgga tcgccgccag gtggtcgccg cgtgcgatga
     8101 ggacgacgtc gtggccggcg cggtgcagga gtgcccccac gacaccgccg acggcgcccg
     8161 caccgtagac gacgtaccgc atgtcgtcac gctatcgcca ggcccagccg cgcctcgggg
     8221 acgaacggtg accaccgccg gctgggtgtc agcgctgcca gctgccaccg accatgactc
     8281 gctgcagctg gagggcttcg tcgaccacga gcacgtccgc ccgcttcccg ctggagagtg
     8341 agccgatctg tgcatcaagg ccgagcaccc tcgcaggtgt ggacgaggcg gcccggactg
     8401 cctcgacgag tcctaccggc gtggcggccg tacgacgtac gacgtcgagc aggtgcgcag
     8461 tcccgccggc gatcgcagcc gtagcgtccg cctccgtcag cgtgtcggga tccccctcat
     8521 ccggcggtgc cagccgggcg accccgtcgc ggacggcgac gcgctgcggc ccgagctcat
     8581 agtcgccgtc ggagacgccg gctgccgcca tggcgtcggt caccagtgcg acatggcccg
     8641 gcgacgccgt ggcgaagacc agctcgacga aggcgtcgtc gaggtgcacg ccgtcggcga
     8701 tcagctcgac cgtcgcctcg ccacgagcgg cagcggtgag agcggcaccg acaggtcccg
     8761 gggcgcggtg gtgcagtgca ggcatgccgt tcgccaggtg ggtcaccagc gccgacccct
     8821 cgagtgctgc cagcgccgct gcgaacgtcg cgtggtcggc gtcgctgtga ccgagcgcga
     8881 cgacgacccc ggctgctctg aggaccgaga cgaccgcgag tgcgtc
//
LOCUS       DATBFZ010000420         9217 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_1602247, whole genome shotgun sequence.
ACCESSION   DATBFZ010000420 DATBFZ010000000
VERSION     DATBFZ010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 9217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9217
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1602247"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1057)
                     /locus_tag="VH419_12685"
     CDS             complement(<1..1057)
                     /locus_tag="VH419_12685"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY7044519.1"
                     /translation="MRLVKELFHSTAENGRAALLIVDGEPGVGKSRLGWEFFKYVDGL
                     TQDTYWHQGRCLAYGEGVAFWALAEAVRLRLVRLAERTGDEDADATELIAEALAALPS
                     TDEERSWLARRLGALLGGGSFGTFAREELFAAWVTFFERVSGGDEIVFLIDDAHHADE
                     GLLDFVEYLLETATFPCLVVLLNRPGLLERRPALATNRRAAVLHLPGLAPRDMSTLVN
                     GLVAGLPDEVSAALVARAEGVPLYAVETVRSLIDRDLVVPKGGQYVLADSQLDLSSIG
                     APASLQALVAARLDTLSGDQRRVVNEASVLGATFELAALRELCPEVDVEVAIPELVRL
                     QILTKDTNRLSSEYGQLK"
     gene            complement(997..1887)
                     /locus_tag="VH419_12690"
     CDS             complement(997..1887)
                     /locus_tag="VH419_12690"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY7044520.1"
                     /translation="MIERATCGVCGTAGLADDARFCHECGAQTEARCGSCGETLILGA
                     KFCSACGTKQSNGAVAAITPLARTASSTAVAERRITSVLFGDLVGFTTMSESRDQEES
                     RELLSRLFNGCRQIVGRYGGTIEKFIGDAIMAVWGVPTAHEDDAERSVRAGLELTRMV
                     TDLGADLGIEGLAMRVGIVTGEVAVTIGAEQQGMVAGDAVNTAARVQSVADPGEVWVD
                     ETTRLLTSAAISYLDAGSHTLKGKAEPVPLWSARAVVASIRGTARADGLGFRSSAGTP
                     SCAWSKSSSTPPPRTAGPHS"
     gene            2062..2421
                     /locus_tag="VH419_12695"
     CDS             2062..2421
                     /locus_tag="VH419_12695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015069864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEY7044521.1"
                     /translation="MRIDKDLVAASATPLVLAILADGESYGYAILKRVRSLSQGELEW
                     SDGMLYPLLHRLGRLGYVTTEWSTPPEGRRRKYYVMTDKGRAALADHQRQWAAVTRAL
                     DVVWPKSGALLTAPGEA"
     gene            2424..3752
                     /locus_tag="VH419_12700"
     CDS             2424..3752
                     /locus_tag="VH419_12700"
                     /inference="COORDINATES: protein motif:HMM:NF038403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HEY7044522.1"
                     /translation="MESQIAEWRTFVAGAPAVNGHDVDELEDHLRHQIAELNAAGLTD
                     DEAFLVAVKRMGDLDAVSREFAREHSDRLWKQLLGPGEDASATPSGWVEAVVFAVAAA
                     VVIELARLLAGFPDKEPTWFARNLSLFVLPLLAGYFARRRQLNTRAWMLAATPFALAA
                     LVINVYPYDPDSATEILVALHLPVVMWFAIAFPYMDGTLQSHKRRMDFVRFTGEWFIY
                     YVLIALGGGVLMGLTAGIFNPAGVDVDQIAAWVLPAGAAGGVIVAAWLVESKQRVVEN
                     MAPVLTMLFTPLFAFMLACAAVVYAVTRVGDAFDRDLLTVFDALLLVVLALVLYAMSA
                     RDPSTTPNWMDWIQLVAVVSALVLDLMVLGSMVARIGDLGFSPNRTAALGLNVVLLVN
                     LAGAALLSVRFVTRRSSLHRLERWQTSYLPVFALWAATVVVVLPPLFGFS"
     gene            complement(3977..4546)
                     /locus_tag="VH419_12705"
     CDS             complement(3977..4546)
                     /locus_tag="VH419_12705"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEY7044523.1"
                     /translation="MPELMVDFITSLEGCASAEGWPGWWGLEGPEYLAWLGEQPEYTI
                     LMGAKTYRLMSGFSESGEEGVGALDEMSKVVFSSTLEAPLTWANSTLVSEDPVEAVRA
                     MKQNGDTELRTVGSLMLCRTLLAAGLVDRFRVVVFPVITGSTGSERIYDGYPDVALEM
                     VDHRTFDGRLQLLEYVPTVLTGPPGSSDG"
     gene            complement(4690..5778)
                     /locus_tag="VH419_12710"
     CDS             complement(4690..5778)
                     /locus_tag="VH419_12710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008360853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY7044524.1"
                     /translation="MPTRTPLSLTLASAAVLVLVGCTGGESTSEPATGSGPTAAEPTA
                     DVATSTDAPGGSSSPVPSPQGPAEPPKGVVDVPRPQSLQAVMARAYHGGSIRTLRETA
                     DYGAYTRSDVRYRSDGVRVTGVLHVPAGRGPFPALVLAHGYIEPSSYVTGQGMSREMD
                     YLARAGYVLLHTDYRGHAGSDPATALDRETRLGYTEDVITAVQAIKRLPYVDPDRVGL
                     LGRSMGGGIVLNVLVTKPGLADAAVVYAPVSSRFLDNFNRWTRPERPDMAAAVLDRLG
                     TPAERPAVYKSLSPRTFFDRITEPVLIHHGTADESCPVRWSRETFRALRAASVDARLQ
                     TYPGEPHAFVADWALSIRRTDRFFDHHL"
     gene            complement(5972..6340)
                     /locus_tag="VH419_12715"
     CDS             complement(5972..6340)
                     /locus_tag="VH419_12715"
                     /inference="COORDINATES: protein motif:HMM:NF014687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HEY7044525.1"
                     /translation="MSEPAQSDDEQSTTTTDPRFQLANERTLLSWLRLSLGLLAGAVA
                     VSSPVSRLGHATRAVLGLWLVAISALAATVGWVRWQRTTRALERGTPIPETSAVRWVA
                     VGWGITIVGVAVAVAIETII"
     gene            complement(6479..6757)
                     /locus_tag="VH419_12720"
     CDS             complement(6479..6757)
                     /locus_tag="VH419_12720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044526.1"
                     /translation="MMDRGHEWGIGDWFVMTAVMVLFWGGLIAILVWLVRDHRNFESA
                     RPSTDRADALLAERFVRGEIDGEEFIRERTLIHADPTQVSPNADRLSP"
     gene            complement(6929..7285)
                     /locus_tag="VH419_12725"
     CDS             complement(6929..7285)
                     /locus_tag="VH419_12725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044527.1"
                     /translation="MITIAFMLLVGFLAVGVIALVIARSATLDHEKTMTRLHESGAQT
                     LVYEVPNGQDPVEVTVALARAGFTAVGDTEKGMCQVLVECPHGRLEDRARVRAVIEQT
                     SGGDAGGAVVQFAGER"
     gene            7597..8100
                     /locus_tag="VH419_12730"
     CDS             7597..8100
                     /locus_tag="VH419_12730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020580123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigE family RNA polymerase sigma factor"
                     /protein_id="HEY7044528.1"
                     /translation="MASSLRERVEFAEFATALTPRMFRKALLLCGEWHLAEDLVQTTW
                     TNLYRSWRKVDRADNPAAYALAALTKTFLSHKRSRSSSERPQLVVIDQAQSDPDLATR
                     MAVLDALSKLGTLDRTIIVLRYWEDQSVEQTAAAVGLSPGAVKTRSHRALTVLRKQLT
                     SMEEEAS"
     gene            8097..8966
                     /locus_tag="VH419_12735"
     CDS             8097..8966
                     /locus_tag="VH419_12735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044529.1"
                     /translation="MTDDTRPDRDDPVFEFGETLLRKQLDVASRGAMPDVDRIIRTSE
                     SEARKQRTRGRLMLVAGVAAASVLTIWGVSQVDLGSDRNGNGVQPATHGPTTDAGETV
                     VDGTARALAAAVIDHLDGAQIESIEGLTVDSGPAQQLSVKIWLRYDGVPATVLVTAWQ
                     PDAEGYDPGAVSCSTMTPVDHSHDTIQDPPADIESFGCTPSESFPGQSPEWWLAADTV
                     QPDPTADQVAKRITLWVQTDQADTDIDRLRPGLDQLREIALDDAVGAYTTQDKIDEGQ
                     TLPSYTDVTEFSD"
     gene            complement(9051..>9217)
                     /locus_tag="VH419_12740"
     CDS             complement(9051..>9217)
                     /locus_tag="VH419_12740"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY7044530.1"
                     /translation="AWRGSHARTPDASAGSGLGLAIVRGIVDAHHGTVRVDNVGDGCR
                     FLVRLPAAAG"
BASE COUNT         1512 a         3158 c         3084 g         1463 t
ORIGIN      
        1 acttcagctg gccgtactcc gaactgaggc ggttggtgtc cttggtcagg atctgcaggc
       61 gcaccagctc tgggatcgcc acctcgacgt cgacttcggg gcagagctcg cgcaatgccg
      121 ctagctcgaa ggtcgcgccg aggacgctgg cttcgttgac caccctgcgt tggtcgccgg
      181 acaacgtgtc cagccgtgcc gccaccaggg cctgcagcga tgccggcgcg ccgatcgacg
      241 acaggtcgag ctgggagtcg gcaaggacgt actggccacc cttggggacc acgaggtctc
      301 ggtcgatgag ggagcgaacc gtctccacgg cgtacagcgg caccccctct gcgcgggcga
      361 cgagggctgc cgacacctcg tcgggcaagc cggcgaccaa gccgttgacc agcgtcgaca
      421 tgtccctcgg cgcgagcccg gggaggtgga gcaccgccgc gcggcggttc gtcgccaatg
      481 cgggccgacg ttccagcaac ccgggacggt tgagcaggac caccaggcac gggaaggtcg
      541 cggtctcgag aaggtactcg acaaagtcca gcagtccctc gtcggcgtgg tgcgcatcgt
      601 cgatgaggaa gacgatctcg tcgccgccgc tgacgcgctc gaagaaggtg acccaggcgg
      661 cgaagagctc ctcgcgggcg aacgtcccga acgaaccgcc ccccagcagg gctcccagcc
      721 tgcgcgccag ccaactgcgc tcctcatcgg tggacggcaa ggcggccaag gcttcggcga
      781 tgagttctgt tgcgtcggcg tcctcatcac ccgtacgttc ggccagccgg accaggcgca
      841 gacggactgc ttcggccagc gcccagaacg cgacgccctc gccatacgcc aggcagcgcc
      901 cctggtgcca gtacgtgtcc tgcgtgaggc cgtcgacgta cttgaagaac tcccagccaa
      961 ggcggctctt gccgacacca ggctctccgt ccacgatcag gagtgcggcc cggccgttct
     1021 cggcggtgga gtggaagagc tctttgacca ggcgcagctc ggggtcccgg ccgacgaacg
     1081 gaacccgagc ccgtccgccc gcgccgtgcc acgtatcgac gcgacgaccg cccgagcaga
     1141 ccacaacggc accggttcag ccttgccctt gagggtgtgg cttccggcgt cgagatagga
     1201 gattgcggcc gacgtgagca gcctggttgt ctcgtccacc cagacctcgc cagggtcggc
     1261 gaccgactga acccgagcgg ctgtgttgac ggcgtcaccg gcaaccatgc cctgctgctc
     1321 cgcaccgatg gtcaccgcga cttcaccagt cacgatcccg acacgcatgg cgagtccttc
     1381 gatgccgaga tccgcaccga ggtcagtcac catccgagtc agctcgagac cggcacgtac
     1441 cgagcgctcg gcgtcgtcct cgtgggcggt cggtactccc cagaccgcca tgatggcgtc
     1501 gccgatgaac ttctcgatcg tgccgccgta acgaccgacg atctggcggc acccgttgaa
     1561 caggcgcgac aacagctccc gggactcctc ctggtcacgg gattcggaca tggtcgtgaa
     1621 cccgacgagg tcgccgaaga ggacgctcgt gatccgtcgc tcggcgacgg ccgtggacga
     1681 cgcggtcctt gctagcggcg tgatcgcagc gactgcccca ttgctctgtt tcgtgccgca
     1741 agcgctgcag aacttggctc ccaggatcag cgtctcgccg caggacccgc agcgcgcctc
     1801 ggtctgcgca ccacactcgt gacagaagcg ggcgtcatcg gccaacccag cggtaccgca
     1861 aaccccacaa gtggcccgct cgatcatgcg ggccactgta agggcggggg atgatcgtcc
     1921 gcagcgggtt ctgcgatcgg tacgagccgg gcgtactgct ccggctcctg catacagtct
     1981 cttgagctgt cgacaacggg cttgcggact tcggacacct tgtggttcta tgcatggtag
     2041 ttcgagccat tggagcggga cttgcgcatc gacaaagacc tcgtcgcggc ttctgcgaca
     2101 ccgctggtgc tggcaatcct ggccgacggc gagagctacg ggtacgcaat cctcaagcgc
     2161 gttcgttcgc tctcgcaggg ggagctcgag tggagcgacg ggatgctcta cccgctcctc
     2221 caccggctcg gtcgtctcgg gtacgtgacg acggagtgga gtacgccgcc ggaagggcgc
     2281 cgccgcaagt actacgtgat gaccgacaag ggtcgagcgg cgctggccga ccaccagcgg
     2341 cagtgggcgg ccgtcacgcg ggctctcgat gttgtctggc cgaagtccgg ggcgctcctc
     2401 accgctcccg gcgaggcgtg agcgtggagt cgcagatcgc cgagtggcgg accttcgtcg
     2461 ccggcgcgcc agccgtcaac ggccacgacg tcgacgagct cgaggaccat cttcgccatc
     2521 agatcgcgga gctgaacgcg gccggactca ccgacgacga ggcgttcctg gtcgcggtga
     2581 agcggatggg cgacctggac gccgtgtccc gagagttcgc acgtgagcac agtgaccggc
     2641 tgtggaaaca gctgcttggg ccgggcgagg atgcgtctgc gactcccagc ggctgggtcg
     2701 aggcggtggt cttcgccgtt gccgccgccg tggtgatcga gctcgcccgc ctccttgcgg
     2761 gctttcccga caaggagccg acctggttcg cgcggaacct cagcctgttc gtcctgccat
     2821 tgctggccgg gtacttcgct cgccggcggc agctgaacac gcgcgcgtgg atgcttgccg
     2881 ccacgccgtt cgcgctcgcc gcgctcgtga tcaatgtcta tccctacgac ccggactcgg
     2941 ccaccgagat cctcgtcgcg cttcatcttc cggtggtcat gtggttcgcg atcgccttcc
     3001 cgtacatgga cgggacgctc caatcgcaca agcggcgcat ggacttcgtc cgcttcacgg
     3061 gcgaatggtt catctactac gtgctgatcg ccctcggcgg gggcgtgctg atgggcctga
     3121 ccgccgggat cttcaacccc gcgggggtgg atgtcgacca gatcgcagcg tgggttctgc
     3181 ctgcgggcgc cgcgggtggc gtgatcgtgg ctgcgtggct cgtcgagtcg aagcaacggg
     3241 tcgtggagaa catggcgccg gtgctcacga tgctcttcac gccgctgttc gcattcatgc
     3301 tcgcgtgcgc cgccgtcgtc tacgccgtga cgagagtcgg cgacgcgttc gaccgcgacc
     3361 tgctgactgt cttcgacgcc ttgttgttgg tcgttctcgc gctggtcctc tacgcgatgt
     3421 cggcgcggga tccgtcgacg acgccgaact ggatggactg gatccagctg gtcgcggtcg
     3481 tcagcgccct cgtgctcgac ctgatggtgc tgggttcgat ggtcgcgcgg atcggcgatc
     3541 tcgggttcag tccgaaccgg acggcggcgc tgggactcaa cgtcgtcctc ctcgtcaacc
     3601 tcgccggcgc ggccctgctg tccgtgcgat tcgtcacgcg acgcagctcg ctgcatcgcc
     3661 tcgagcggtg gcagacgagc tacctcccgg tcttcgcgct gtgggcagcg acggtcgtgg
     3721 tcgtcctgcc gccgctgttc ggcttctcct gatcgtcacc gtcggcgcga acgccggtga
     3781 accgccccga tctgcctggg tcccgcggca cacccacccc tcccaaggca ggtctgcctg
     3841 gctacccgtc cgtttgcatg gatacccagg caaaccccac gggcaggtgt ccgtatttgg
     3901 tggtgaaggt ggcgttccgg aggcaaggcg gaggtctgac gagtgggcgg atgtgcgaac
     3961 ccgccagacg tcgtggtcag ccgtcggagg atcctggcgg gccggtcagc acggtgggaa
     4021 cgtactcgag cagttgcagc cggccgtcga aggtgcggtg gtcgaccatc tcgagagcca
     4081 cgtccggata cccgtcgtag atccgctcac tgccggtgct cccggtgatc accggaaaga
     4141 cgaccacgcg gaaacggtcg acaagaccgg ccgccagcag ggtccggcac agcatcaggc
     4201 tgcccaccgt gcgcagctcg gtgtcgccgt tctgcttcat cgcgcggacc gcttcaaccg
     4261 ggtcctcgga gacgagcgtc gagttcgccc acgtcagcgg cgcctcgagc gtggacgaga
     4321 acaccacctt cgacatctcg tccagcgcgc cgacgccttc ctccccactc tcggagaacc
     4381 cggacatcaa ccggtaggtc ttcgcaccca tcaagatcgt gtactccggc tgctccccca
     4441 gccaggcgag gtactccggc ccctccagtc cccaccagcc cggccagcct tcggccgagg
     4501 cgcagccctc cagggaggtg atgaagtcca ccatcagttc tggcatcggt gatccttcgc
     4561 tggtcgattg gctgttgctc agccctcccg tcccacgcta gggcgaggga acgtccgccc
     4621 tccagagcca attcgcgccc cgacgatcgg accgttcggc atgcctggcc ggccgcacgg
     4681 ccccacgact cacaagtggt ggtcgaagaa ccggtccgtc cggcggatcg acaaggccca
     4741 gtcagccacg aacgcgtgcg gctcgccggg gtaggtctgc agtcgagcat cgaccgaggc
     4801 ggcacgcaac gcgcggaatg tctcacgtga ccagcgcacc ggacacgact cgtcggctgt
     4861 gccgtgatga atcaggaccg gttcggtgat ccggtcgaag aacgttcgcg gtgacaggct
     4921 cttgtagacc gccgggcgtt cggccggcgt gccgagccgg tcgagaaccg ccgccgccat
     4981 gtccggccgc tcgggccgcg tccaccggtt gaagttgtcg aggaagcgcg agctcaccgg
     5041 ggcgtacacg acagcggcgt cggcgagacc cggcttcgtg accaggacgt tcagcacgat
     5101 cccgccaccc atcgagcgtc cgaggaggcc gacgcggtcg ggatcgacat acggcagtcg
     5161 cttgatcgcc tgcacggccg tgatcacgtc ctcggtgtac ccgagccgcg tctcccggtc
     5221 gagtgctgtc gcggggtccg acccggcatg accgcgatag tcggtgtgca gcaacacgta
     5281 acccgccctc gccaggtagt ccatctcccg cgacatgcct tgcccggtga cgtagctgct
     5341 cggctcgatg tacccgtgcg cgagtacgag tgctgggaac gggccgcgac ctgccgggac
     5401 gtgcaggaca ccggtcaccc ggacgccgtc actgcgatag cgcacgtcgc tgcgggtgta
     5461 tgccccgtag tccgcggtct cccgcagtgt gcgaatcgac cctccgtggt acgcccgggc
     5521 catcacggcc tggagagact gcggacgagg tacgtcgacc acccccttgg gcggctcggc
     5581 cggaccttgc ggcgaaggaa ccggcgagct cgacccgccg ggtgcgtcgg ttgaggtcgc
     5641 aacgtcggct gtcggctccg cggccgtcgg gccggacccg gtcgcgggct cagacgtcga
     5701 ctcgcccccg gtgcacccga ccagcacgag cacggcggcg ctcgccaggg tcagggacag
     5761 gggagtccgg gttggcacgg gtccgattct gcctcctccg acagaagacg acagccgccg
     5821 aaacgtcgct gcctgggagg cggcctcgac ctcctcgaac gctgaccttc cagcgcacgc
     5881 tgcgcattcc cgagggcggg ctgcgccagc tgtcgcctgg gtattgcccg ggatctggtg
     5941 atcgccggcg caccttgggg gccaggtccc actagatgat ggtttcgatg gccacggcca
     6001 cggcgacgcc gacgatcgtg attccccagc cgacggcaac ccatcgcacg gcgctggtct
     6061 ccgggatggg agtaccacgt tcgagggccc gtgtggtgcg ctgccaacga acccaaccca
     6121 cagtggcggc gagcgcgctg atggcgacca gccagaggcc gagtacggct cgtgtcgcat
     6181 gcccgagccg agacaccgga ctgctcacgg cgacagctcc cgccagcaag cccagcgaca
     6241 gtcgcagcca cgacagcagg gtgcgttcgt tggccagctg gaagcgtgga tccgtcgtgg
     6301 tcgtggactg ctcgtcgtcg gactgcgccg gctcactcat cgtgtcgtct cctggtcggc
     6361 ggaccgcatc gtcgttgcac caatccgcga gccctcgtgg gcggtccacg cccgacggta
     6421 cgccgcaacg atcggcaagg ggacaccgac gttcaccaca ggccgcccaa cggccccgtc
     6481 aaggtgagag gcgatccgcg ttcggagaca cctgggtcgg atcagcgtgg atgagagtgc
     6541 gctcgcggat gaactcttcg ccgtcgatct cgccgcggac gaaccgctcg gccaacaacg
     6601 catcggcacg atccgtcgac ggcctggcgg actcgaagtt gcggtggtcg cgaacgagcc
     6661 agaccagtat ggcgatcagc ccgccccaga agagaaccat cacggccgtc atcacgaacc
     6721 agtcgccgat accccactcg tgaccccgat ccatcatggg actcacctcc tcgtcgaggc
     6781 ccggcgactc ccggtctcga ccccgacatc atgggtccgc ggcactcgac gtcggtaggg
     6841 ccgaaggcaa cgtcattcgg gtccaggtgc gcgggtgccg cggggtcagt caacgtcgtg
     6901 cgcggagcgg aacgaccgcg gtcagcgatc accgctcgcc ggcgaactgc accacggcgc
     6961 cgcccgcgtc gcctccactc gtctgctcga tgaccgcccg aacgcgagcc cggtcctcga
     7021 gccgaccgtg cggacactcc acgagcacct gacacatgcc cttttccgtg tctcccacag
     7081 ccgtgaatcc cgcgcgcgcc agggccactg tcacctcgac ggggtcctga ccgttcggga
     7141 cctcgtacac caaggtctgc gcaccggact cgtgcagccg cgtcatggtc ttttcgtggt
     7201 cgagggtcgc ggaacgtgcg atgacgagcg cgatgacgcc gaccgccagg aaaccaacca
     7261 gcagcatgaa agcaatcgtg atcatgacga cctccgatcg ggtcgaaccg cccgatcggc
     7321 accctcaccg ggcggccgcc tcaactgatg tctcgagtct tcgtttggca caagcgtcca
     7381 gcgcccgtgt gccatgcgga agggtcttga gtcacgtcca acaggtcgta ggtcctgtgc
     7441 tgacgcacga ggagcgcagc tccgccctgc tcgtgtcgac ggacgcccga gacgacgtcg
     7501 gaaaaccccg gggggagtcc gccttcggag gctctgtaac cgaatccgtg cctgctgcgt
     7561 ctagaggggc aagatgtcga ggacaggagg cggacgatgg catcttcatt gcgcgaacgc
     7621 gtcgagttcg cagagttcgc cactgccctg acgccacgga tgtttcgcaa ggcgttgctg
     7681 ctgtgcgggg agtggcacct ggcagaggac ctggtccaga ccacgtggac gaacctgtac
     7741 cgctcatggc ggaaggtcga ccgggcggac aacccggcgg cgtacgccct ggcggcgctg
     7801 accaagacct tcctgagcca caagaggtct cgatcgagca gtgaacgccc acaactcgtg
     7861 gtgatcgacc aggcccagag cgatccggat cttgccactc ggatggccgt gctcgacgcg
     7921 ctgagcaagc tcggcacgct cgaccggacg atcatcgtgc tccgctactg ggaggaccag
     7981 tcagtggagc agaccgcggc cgccgtgggg ttgagccccg gcgcggtcaa gactcgcagc
     8041 caccgtgcgc tcaccgtgtt gcgcaagcaa ctcacgtcga tggaggagga ggcgtcatga
     8101 ctgacgacac gcgacccgac cgggacgacc ctgtgttcga gttcggcgag acgttgcttc
     8161 gcaagcagct cgacgtcgcc tctcgcggcg caatgcccga tgtggaccgg atcatccgga
     8221 cgagcgagtc ggaggcgagg aagcagcgta cgcggggccg gctgatgctg gtggcgggtg
     8281 tggctgccgc gtcggtactg acgatctggg gcgtcagcca ggtcgacctc gggagcgatc
     8341 gaaacggcaa cggggtccag ccggcgactc acggaccgac gaccgatgct ggtgagacgg
     8401 ttgtcgacgg gaccgcgcgg gcgttggccg cagcagtcat cgatcacctc gacggcgcac
     8461 agatcgagtc gatcgagggc ctgaccgtcg actccggccc ggcacagcag ctctcggtga
     8521 agatctggct gcggtatgac ggggttccgg cgaccgtgct ggtgaccgcc tggcaaccag
     8581 acgcagaggg gtacgacccg ggcgcggtct cgtgctcgac gatgacaccg gtcgaccact
     8641 ctcacgacac gatccaggac ccgccggccg acatcgagag cttcggctgc acgccgagcg
     8701 agtcgttccc cggtcagagt cccgaatggt ggctggcggc ggacacggtg cagcccgacc
     8761 cgaccgcaga ccaggtggcg aagcggatca cgctgtgggt gcagacggat caggcggaca
     8821 ccgacatcga ccggctgcgg cccgggctcg accagctccg cgagatcgca ctcgacgacg
     8881 cggtcggcgc ctacacgacg caggacaaga tcgacgaagg ccagacgctg ccgtcgtaca
     8941 ccgacgtcac ggagttcagt gactagtcga gctcacggcc gccaggtgta cgcgcctcag
     9001 caggccccgg acactcccca cacgtccggg gcctgctcgg ggccgtcgtc tcagccggcg
     9061 gccgcgggca gccggacgag gaagcgacac ccgtcgccga cgttgtcgac tcgtaccgtc
     9121 ccatgatggg cgtcgacgat tccgcgcacg atcgccagac caagcccgga tccggccgat
     9181 gcgtcgggtg tccgggcatg actaccgcgc cacgcga
//
LOCUS       DATBFZ010000421         4330 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_5072150, whole genome shotgun sequence.
ACCESSION   DATBFZ010000421 DATBFZ010000000
VERSION     DATBFZ010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 4330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4330
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5072150"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1816)
                     /locus_tag="VH419_12745"
     CDS             complement(<1..1816)
                     /locus_tag="VH419_12745"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HEY7044531.1"
                     /translation="MNARIVSFESRPAARPIHHARGLLAVVVAAAAVAAWPGTAAAAI
                     TTTAATIDGVTSTSAPPGSVMNASVTADVSGAAWSATSVQFGSENADCINHDNESSGT
                     GRKASFKVTAPGDPGDYDVGFTPNTSSSCDDTAGSTFTLTKGLTVTTPAANPNLPPRC
                     GINVMLVLDESGSIETSGATEKVKSAATAFLNALSGTGAKVSITDFSTTAAQQVPYTT
                     VTSDSIADIFDPYLKTGYKPSGWTNWEAAFQTVHQANKSTLADLVVFITDGDPTARNN
                     PGHSPVTNLTEGDVTAMRAAATEADLVKGDGSHVLAMGVGAAVTKESSARRLTAVSGF
                     DETPPAEFSEADFTLVTDFDKLAAALRRIAVELCQASVTVTKLVDEGDGVYRPDQGWE
                     FTTTVSTSPGGFAWVLPTSGSGASRTGTTNDDGVTTFQWKPENATATSTVTIEETLKP
                     GFDFVDADCKVNSVSSSGSRRRVLRRTKTTGNPKATATLAPGEYATCTVRNKITPGTI
                     EIEKQATPQSSQRFLFGGSLGAFTLVDDGKSTSSSKVFEQLTPGTYTVSELVPEDWEL
                     TGIACVPASAATIAGTQVTITLAPAGSVTCTYSDNKVEP"
     gene            complement(1913..4054)
                     /locus_tag="VH419_12750"
     CDS             complement(1913..4054)
                     /locus_tag="VH419_12750"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044532.1"
                     /translation="MIVARVTGAAALAASLLLLLLAPRASADPDTTKLVTIAARSCPA
                     YTAIFGNRARNDIMESLKNLGPDTNYADGEAVSVEKENEFPQSLCSPITGWQFKLGKG
                     YQTRAVSGSWGSLSIVTSPFDRSVVTKASVPQLNDQGQEIPGKSVAGAVTIELTKEQA
                     ELAGQSNALWIQGGLTDDPVLDKPYPGQYGFGALRCAIDDLNGDNVEWIGYPSGARHV
                     FCFAYYVQPPPTSGTIVVRKEVDPASSPPSETFPFVGNISFNDDKTFTLNAAPDAPGA
                     MTFYRAGGSSWNFTEQVPAGWQLTNITCTSATGASTSTTSLASGTTTVALAADDTVTC
                     TYRDRLTPPPAGLLVRKITRGGLGTFGFTVDPEAGGEARSGKATTTREGVAAPALPLF
                     GDLDPGAYTVTEKPPSSDAGSWALERVTCNSTPRTSRASSQRVVLQPGTGTICTYENR
                     FTPNGTIRLRKVTRNGTATTSFVINTVGVTPPRQFRQSATTTAENESVLATGSNTSSL
                     PLGTYDITELSAPSTATEAWRLDTVTCNGVAVPAEQGSLRVTLTADHPDVDCTFTNVL
                     DKAKVLPDTATSGDSQTTPIADLVVTKRPDRHSVVLGDTVRYTVRVTNRGPATAHDVV
                     VAEEIGNRHAIISASPAKYRCSTKRKLPSCLIGTLRSGQTATIHVVARSLVSGLRPNR
                     AVVLSSTSERTLRNNVARAAVLVRPPTSFTG"
     gene            complement(4087..>4330)
                     /locus_tag="VH419_12755"
     CDS             complement(4087..>4330)
                     /locus_tag="VH419_12755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HEY7044533.1"
                     /translation="GTDRLIVHDARCEDPSLAFALARLGVLGAGPLPLGVFRAVDAPP
                     WGDGLVAQLREARAQVGEADLEALLHAGDTWTIANR"
BASE COUNT          581 a         1407 c         1584 g          758 t
ORIGIN      
        1 gaggctcgac cttgttgtcg ctgtaggtgc aggtgacgga gcccgccggg gcgagcgtga
       61 tcgtcacctg cgtgcccgcg atggtcgccg ccgaggccgg gacgcacgcg atgccggtga
      121 gctcccagtc ctcggggacc agctcgctga ccgtgtaggt gcccggcgtc agctgttcga
      181 agaccttcga cgacgaggtg ctcttgccgt catcgacgag cgtgaaagcg ccgagcgacc
      241 ctccgaacag gaagcgctgc gagctctggg gcgtcgcctg cttctcgatc tcgatcgtgc
      301 ccggggtgat cttgttgcgc acggtgcacg tcgcgtactc gcccggggcc agcgtcgcgg
      361 tggccttcgg gttgccggtg gtcttggtgc ggcgcaggac gcgcctgcgg ctgccgctcg
      421 aactcaccga gttgaccttg cagtcggcgt cgacgaagtc gaagccaggt ttcagggtct
      481 cctcgatcgt gaccgtgctc gtcgccgtcg cgttctcggg tttccactgg aaggtggtca
      541 caccgtcgtc gttcgtcgtg cccgttcgcg atgctccgga cccgctggtc ggcaggaccc
      601 acgcgaagcc gccggggctg gtggagaccg tggtcgtgaa ctcccagccc tggtccggcc
      661 ggtagacgcc gtcgccctcg tccacgagct tggtcaccgt caccgatgcc tggcagagct
      721 cgaccgcgat cctccgcagc gctgcagcca gcttgtcgaa gtccgtcacc aacgtgaaat
      781 cggcctccga gaactcggcc ggcggggtct cgtcgaagcc ggagacagcg gtaagccgtc
      841 gcgcgctcga ctccttggtc acggccgcgc cgacccccat ggccagcacg tgcgagccgt
      901 ctcccttgac gaggtccgcc tcagtggccg ctgcgcgcat cgccgtgacg tcgccctcgg
      961 tcagattggt gacggggctg tggcccggat tgttccgcgc ggtgggatcg ccgtcggtga
     1021 tgaagacgac gaggtcggcg agggtgctct tgttcgcctg gtgcacggtc tggaatgctg
     1081 cttcccagtt ggtccagccg ctgggcttgt agccggtctt gaggtagggg tcgaagatgt
     1141 cagcgatgga atcggacgtg accgtcgtgt acggcacctg ctgcgcggcc gtcgtgctga
     1201 agtcggtgat cgagactttc gccccggtgc ccgaaagagc gttcaggaag gccgtggccg
     1261 ccgacttcac cttctccgtc gcccccgacg tctcgattga gcccgactca tcgagcacca
     1321 gcatcacatt gatgccacag cgcggcggaa ggttggggtt cgcagcgggc gtggtgacgg
     1381 tgaggccctt cgtcagggtg aacgtgctgc ccgcggtgtc atcgcagctg ctggacgtgt
     1441 taggtgtgaa tcccacgtcg tagtcgccgg gatcaccggg cgcggtcacc ttgaagctgg
     1501 ccttcctgcc ggtaccggag ctttcgttgt cgtggttgat gcagtcagcg ttctccgatc
     1561 cgaactgcac cgaggtggcg ctccacgccg cgccgctgac gtcagccgtc accgaggcgt
     1621 tcatcacgct cccgggagga gcagaggtgc tcgtcacgcc gtcgatcgtg gcggcggtgg
     1681 tggtgatcgc cgccgcggcc gtcccgggcc acgccgcaac tgctgcggcc gcgaccacga
     1741 cagcaaggag cccgcgcgca tggtggatgg gccgcgcagc cggcctgctc tcgaacgaga
     1801 cgatccgtgc gttcacctcg ggcctccctc ttcctgctgc ctagcccttc ccggcgggga
     1861 tgctttcagc ggccggggtg agcagccatc accccgaccg ggtgatatcg ccctaccccg
     1921 tgaagctcgt cggcggcctg acgaggacgg cggcgcgcgc cacattgttg cgcagggttc
     1981 gctcggaggt tgacgagagg accaccgcgc ggtttggccg caggccggag accagcgagc
     2041 gagcgacgac gtggatggtc gcggtctgcc cgctgcgcag cgtcccgatc aggcagctgg
     2101 gaagcttgcg cttggtcgag cagcggtact tcgccgggct ggccgagatg atcgcgtggc
     2161 ggttgccgat ctcctcggcg acgacgacgt cgtgtgcggt ggccgggccg cggttcgtga
     2221 cgcggaccgt gtagcgcacc gtgtcgccta gcacgacgct gtgccggtcg ggccgcttgg
     2281 tcacgacaag atcggcgatc ggcgtcgtct gcgagtcgcc gctcgtggcg gtgtcaggga
     2341 gcaccttggc cttgtcgagg acgttggtga aggtgcagtc gacgtcgggg tggtcggcgg
     2401 tgagcgtgac gcgcaggctg ccctgctcgg cggggacggc gacgccgttg cacgtcaccg
     2461 tgtcgagtcg ccaggcctcc gttgcggtcg atggcgcgct gagctcggtg atgtcgtagg
     2521 tccccagcgg caggctggag gtgttgctgc cggtcgccag cacggactcg ttctccgccg
     2581 tcgtcgttgc ggactgccgg aactggcgtg gcggagtgac gccgaccgtg ttgatgacga
     2641 agctggtggt ggcggtgccg tttcgggtca ccttgcgcag gcggatggtg ccgttgggcg
     2701 tgaagcggtt ctcgtacgtg cagatggtcc cggtgcccgg ctgcaggacg acgcgctgcg
     2761 aggacgcgcg gctggtccgg ggcgtggagt tacaggtcac gcgttccagc gcccatgagc
     2821 ccgcgtcgga actcgggggc ttctcggtga ccgtgtaggc gccgggatca aggtcgccga
     2881 acaggggcag cgcaggcgcc gccacgccct cgcgggtggt ggtggccttc ccgctccggg
     2941 cctcgccccc cgcctcgggg tcgaccgtga agccgaaggt gcccagtccg ccgcgcgtga
     3001 tcttgcggac caggagcccg gcgggcgggg gcgtgagccg atctctgtac gtacacgtga
     3061 ccgtgtcgtc cgccgcgagc gcgaccgtcg tcgtgccgct cgccaggctc gtggtgctcg
     3121 tactggcccc tgtggccgac gtgcaggtga tgttggtcag ctgccagccc gcgggcacct
     3181 gctcggtgaa gttccagctg gacccaccgg cgcggtagaa ggtcatggcg ccgggggcgt
     3241 cgggcgccgc gttgagcgtg aacgtcttgt cgtcgttgaa cgagatgttg ccgacgaagg
     3301 ggaaggtctc gctgggtggc gaggaagcgg gatcgacctc cttgcggacg acgatcgtcc
     3361 cgcttgtcgg cggcggctgg acgtaatagg cgaagcagaa gacatgccgc gctccgctcg
     3421 gatagccgat ccactcgacg ttgtcgccgt tgaggtcgtc gatcgcgcag cgcagcgccc
     3481 cgaagccgta ctggccgggg tacggcttgt cgagcaccgg gtcatccgtc agcccgccct
     3541 ggatccatag ggcgttcgac tgcccggcga gctcggcctg ctccttcgtc agctcgatgg
     3601 tgacggcgcc cgcgacgctc ttgcccggta tctcctggcc ctggtcgttg agttgcggga
     3661 ccgaggcctt ggtgaccacc gacctgtcga acgggctcgt gacgatcgac agcgaccccc
     3721 aggagccgct cacggcgcgc gtctggtagc ccttgccgag cttgaactgc cagccggtga
     3781 tcggcgagca caagctctga ggaaactcgt tctccttctc gaccgagacg gcctcgccgt
     3841 cggcgtagtt ggtgtccggg ccgaggttct tgaggctctc catgatgtcg ttgcgggccc
     3901 ggttgccgaa gatggcggta tacgccggac acgaacgcgc ggcgatcgtc acgagcttgg
     3961 tggtgtcggg atccgcagac gcgcggggcg ccagcagcag caggagaagg ctcgcggcca
     4021 acgccgccgc ccctgtcacg cgcgccacga tcatggcggc aacctaccag ccgtctgcgg
     4081 acggactcag cggttggcga tcgtccaggt gtcgcccgcg tgcagcagtg cctcgaggtc
     4141 ggcctcgccg acctgcgcgc gcgcctcgcg cagctgggcg acgagtccgt cgccccacgg
     4201 cggggcgtcg acggcgcgga agacgccgag cggcagcggg cccgcgccga gcacgccgag
     4261 gcgagcaagg gcgaaggcca gcgacgggtc ctcgcagcgg gcgtcatgca cgatcaggcg
     4321 gtcggtgccg
//
LOCUS       DATBFZ010000422         2865 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_949715, whole genome shotgun sequence.
ACCESSION   DATBFZ010000422 DATBFZ010000000
VERSION     DATBFZ010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2865
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_949715"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="VH419_12760"
     CDS             <1..498
                     /locus_tag="VH419_12760"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY7044534.1"
                     /translation="VVVGGANSAGQAALFLAQHAGRVHVLIRRDDLRDTMSNYLAQRL
                     THHERVTVHPRSELSAVHGTNRLSALSWRDHVLERDVRLDAAGLFLMIGADPCTTWLH
                     EMGVELDPKGFVVTGDGFATSVPGVFAVGDVRAGSVKRVASAVGEGSVVISAVHAYLA
                     DTRRS"
     gene            589..1434
                     /locus_tag="VH419_12765"
     CDS             589..1434
                     /locus_tag="VH419_12765"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY7044535.1"
                     /translation="MGWLEVADGILVRRHASFDLNAGLVVGDGHCLVIDTREHLDAGQ
                     ELAAAVRQVTTAPWTVVNTHAHFDHFMGNGAFLPGEIWSLDRTREIIEHYGDVQRRVM
                     VHVAHQHERADLAAGLEATPLTPPNRTFPPPATALDIGGREVTLRHLGRGHTDNDIVI
                     SVAGTSVVFAGDLVEEGAPPAFDDAYPIAWGATLEALLPLTSGPVVPGHGAVVDEAFV
                     REQRELHDRVAAAARDAATTVRGLPDEVATVALTRARAELAGRLTMPRPDEVLARSGV
                     PDDQS"
     gene            1548..2036
                     /locus_tag="VH419_12770"
     CDS             1548..2036
                     /locus_tag="VH419_12770"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020579880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEY7044536.1"
                     /translation="MGIPDLRAHAHDLLRANRYLTLGTTGTDGRPWTSPVYFAADGVR
                     EFYWMSEIGARHSRNLVDRPQVSLVVFDSTVEPYHGRAVYASGSARELSGDELAAGLA
                     VYAGADAPGVPTTTRDDVTGSSPYRLYQATAADVWVLCPREPRQPCPLHGLARDHRAR
                     ID"
     gene            2011..2157
                     /locus_tag="VH419_12775"
     CDS             2011..2157
                     /locus_tag="VH419_12775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044537.1"
                     /translation="MTIAPASTDRRRLRRSAQISSDRICAMTRFENTVLPWFGLPRSL
                     PDVD"
     gene            complement(2185..2589)
                     /locus_tag="VH419_12780"
     CDS             complement(2185..2589)
                     /locus_tag="VH419_12780"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY7044538.1"
                     /translation="MAAEFLWRGPFTNEEANRLHAEAFETRLCTADEWDWERLVRAHS
                     LGWVTARSADELVGFVNVIWDGLVHAWVQDEMVAKSARGAGIGRELIRVAADGARQAG
                     CEILHVDFDDHLQSFYYQACGFTPTNAGLLRL"
BASE COUNT          457 a         1013 c          957 g          438 t
ORIGIN      
        1 gtcgtggtgg gaggtgccaa ctcggccggc caagccgccc tgttcctggc ccagcacgcc
       61 ggccgggtcc acgtcctgat ccgccgggac gatctccgcg acacgatgtc gaactacctc
      121 gcgcagcggc tcacgcacca cgaacgggtc accgtccacc ctcgctcgga gctgtccgcg
      181 gtccacggca cgaatcggct gagcgcgctg agctggcggg accacgtcct cgagcgggac
      241 gtccgcctcg acgcagccgg cctgttcctg atgatcggcg cagacccctg cacgacctgg
      301 ctgcacgaga tgggcgtcga gctcgacccg aaagggttcg tcgtcaccgg cgacggcttc
      361 gcgacgtcgg tgcccggagt gttcgccgtc ggtgacgttc gcgccgggtc cgtcaagcgg
      421 gtggcctcgg cggtcggcga gggatcggtg gtgatctccg ccgtccacgc ctatctcgcc
      481 gacaccaggc gatcctgacc gtgttcgatc agacatccgg gtccgacctg cgctgctcgg
      541 ccgccgcggg actgaagtga gccctggttc ggtcctaggc tgaccagcgt gggctggctg
      601 gaggtcgccg acgggattct ggtgcgacgg catgcgtcct tcgacctgaa cgccgggctg
      661 gtggtcggcg acggccactg cctggtgatc gacaccaggg aacacctgga cgccggacag
      721 gagttggccg ccgccgtccg tcaggtgacc acagctccgt ggacggttgt caacacgcac
      781 gcgcacttcg accacttcat gggtaacggc gccttcctgc ccggcgagat ctggtcgctc
      841 gaccggaccc gggagatcat cgagcactac ggcgatgtac agcgtcgggt gatggtccac
      901 gtcgcgcacc aacacgaacg tgccgacctg gccgccggac tggaggcgac gccgctgacg
      961 ccgccgaatc ggaccttccc gccgccggcg accgcactcg acatcggcgg acgggaagtg
     1021 acgctgcgcc acctcggccg cggacacacc gacaacgaca tcgtcatcag cgtcgccggg
     1081 acgagcgtcg tgttcgcggg cgacctcgtc gaggaaggcg cgccgcccgc cttcgacgac
     1141 gcgtatccga tcgcgtgggg cgccacgctc gaggcgttgc tgccactgac atccggaccg
     1201 gtcgtccccg ggcacggcgc cgtggtcgac gaagcattcg tccgcgagca gcgcgagctt
     1261 cacgaccggg tcgcagctgc ggcgcgcgat gccgcgacga cggtccgcgg attgcccgac
     1321 gaggtcgcca ccgtcgcgct gacgcgggca cgcgccgagc tggcgggcag gttgaccatg
     1381 ccgaggccgg acgaggtcct ggctcgttcc ggcgtgcccg atgaccagag ctagcagggc
     1441 accgtcgcag cctcacactg aggccatgga tacgtcggcc gggtcgtcga ctcgcgccac
     1501 ccggtgctca cctcgaccag cattcgaccg acaggagaac gtgaaccatg ggcatccctg
     1561 atctgcgcgc ccatgcgcat gacctgctcc gcgcgaaccg ctacctgacg ctgggcacga
     1621 ccggcaccga cgggcggccg tggaccagcc cggtctactt cgccgccgac ggcgtgcggg
     1681 agttctactg gatgtccgag atcggcgccc gacactcacg caatctggtc gatcgcccgc
     1741 aggtcagcct ggttgtgttc gactccaccg tcgagccgta ccacggccgc gcggtgtacg
     1801 cgtccggatc cgcccgggag ctgtccggcg acgagctggc cgcgggcctc gcggtgtacg
     1861 ccggcgcgga cgcccccggc gtgcccacca cgacacgcga cgacgtgacc ggttcgtcgc
     1921 cgtatcgcct ctatcaggca accgcggccg acgtgtgggt gctgtgcccc cgcgaaccca
     1981 gacagccgtg cccgctgcac ggtctcgcgc gtgaccatcg cgcccgcatc gactgatcgg
     2041 cgtcggctgc ggcgatccgc gcagatcagc agcgatcgca tctgcgccat gacgcgcttc
     2101 gagaacaccg tgctcccgtg gttcggcctg ccacgatcgc tccccgacgt cgattgagca
     2161 agcacgtcac catccgccta ctgctcagag tcggagcaac ccggcattgg tgggggtgaa
     2221 cccgcacgcc tggtaataga acgattgcag gtggtcgtcg aaatcgacgt gcaggatttc
     2281 gcacccggcc tgacgagccc cgtcggctgc gacgcggatc agttcgcggc cgatgcccgc
     2341 cccgcgcgcc gatttggcca ccatctcgtc ctggacccac gcgtggacaa ggccgtccca
     2401 gatgacgttg acaaagccca cgagttcgtc ggccgaacgg gcggtgaccc agccgaggct
     2461 gtgcgcacgg acgagacgct cccagtccca ctcgtcggcg gtgcacagac gggtctcgaa
     2521 ggcctcggcg tgcaatcggt tcgcttcctc gttggtgaac ggaccgcgcc acaagaattc
     2581 agcagccatc ccatgaccgt aggagcatcg ccaggcatcg tgcgatcgat ttcggcgtga
     2641 gcgcgcgcca tcatccgctc atgccagata cccgttcgac gccggaccta gtctccaatg
     2701 caaaccttca gaacgatcaa ggaaagcgag tcaatggcaa gaggtcgagt acgtgcactg
     2761 atcggcgtcg gggcggctgc gggagtggcc gccgtcggct tgttcgcagc tacaccggcc
     2821 ttcgccggaa cggatgtgag cgtcggaacc accggcggct cggga
//
LOCUS       DATBFZ010000423         2963 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_4225548, whole genome shotgun sequence.
ACCESSION   DATBFZ010000423 DATBFZ010000000
VERSION     DATBFZ010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2963
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4225548"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(197..613)
                     /locus_tag="VH419_12785"
     CDS             complement(197..613)
                     /locus_tag="VH419_12785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044539.1"
                     /translation="MLSEEPTSGTPGEREVLGREAQGSRGALMAAGGILVAILVAYVV
                     TLVASSPQPQHDSEQFNMFANIKDACENDDGNLVTTDPHGTPMAFATPDGPERYWCLS
                     RRYLTSIGHAPQDPPPPVREVPGPYKWMWPRTTYGQ"
     gene            complement(704..1036)
                     /locus_tag="VH419_12790"
     CDS             complement(704..1036)
                     /locus_tag="VH419_12790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044540.1"
                     /translation="MRRYREYGAPLLDEGIAIRIGNDVGFDEKGYTCRRPSVRRRDCE
                     IVDERRDAARVIDGRRGHSRDDDKAVDVTLGVLMHERPYVGVRDKMREPRPMCFRGVP
                     VAHAEADG"
     gene            complement(996..1274)
                     /locus_tag="VH419_12795"
     CDS             complement(996..1274)
                     /locus_tag="VH419_12795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044541.1"
                     /translation="MTRVLLIGFDPAAVPGQDAAAVSPACEHARRRALCKPEPVLEYF
                     EYVVNLVHVAAPTSAIAFNSSPADTVEAALRALNVHEALPRVRRSPPG"
     gene            1551..1787
                     /locus_tag="VH419_12800"
     CDS             1551..1787
                     /locus_tag="VH419_12800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044542.1"
                     /translation="MQWWYNHTNPYTGHVEGDYRGAQIGIASPEQLFTSSGRVNTVNQ
                     GFDNTGGNGYRYYADVTCVSGGSGAFDFWVQPFQ"
     gene            1800..2042
                     /locus_tag="VH419_12805"
     CDS             1800..2042
                     /locus_tag="VH419_12805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044543.1"
                     /translation="MRRLVVFDRDGTIVATAPHPDDMPPPEPGAPTFLGFSPAEDEQQ
                     DAIDIPEELSSPEGIRELHESYRVEMIGDRPTLTRR"
     gene            complement(2104..2556)
                     /locus_tag="VH419_12810"
     CDS             complement(2104..2556)
                     /locus_tag="VH419_12810"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEY7044544.1"
                     /translation="MVFVATTFRENAEILLRSDDPHNQDEWNVRMLRMPATVISSTLR
                     DTLGWPDATVASGDAADIVGRLKEESSVPLRSQASLSLNWALMAAELVDRVQVTIFPV
                     VSGRTGTSPILGGAGDFDLELLESHTFDGRTQELVYRPTLHRPRKEVV"
BASE COUNT          520 a          964 c          984 g          495 t
ORIGIN      
        1 caaggtgcac gccgcgaccg tcgccgggct ggaggaggtg ctggacaggt tcctcgtgca
       61 cgggcagacg gtgtcctcga tcgtggtcac cagcccggtc cccggacgtc cgctgccgat
      121 cccggactga gggggatcca tcagttcggc cggcgcgccg ccttcgggaa cccctggtca
      181 gtgagggttg acagggtcac tgcccgtagg tggttcgtgg ccacatccac ttgtacggac
      241 cgggaacctc ccggaccggc ggtggcgggt cctgaggcgc gtgaccgatc gaggtcaggt
      301 agcggcgtga caagcaccag tagcgctcag gcccgtcggg tgtcgcgaac gccatcggag
      361 tcccgtgcgg gtcggtggtg acgaggttgc cgtcgtcgtt ctcgcaagcg tccttgatgt
      421 tggcgaacat gttgaactgt tctgagtcgt gctgaggttg gggactcgac gccacaaggg
      481 tcacgacgta ggcgacgagg atggcaacga gaatccctcc cgccgccatg agggcgccgc
      541 gcgatccctg ggcctcgcgg ccgaggactt ccctctcgcc gggcgttccg gaagtcggct
      601 cttcgctgag caccgcccag ccccctcagt cccgcggccc cggagcagcc ggtccacgcg
      661 ccaaccatgc acgccaacca tgaactgggc cgccagacca tcgtcaccca tcggcctctg
      721 cgtgggcaac aggcacgcct cggaagcaca tcggccgtgg ctctctcatc ttgtcgcgaa
      781 ctccgacgta gggacgttcg tgcatgagga ccccgagggt gacgtcgacg gctttgtcgt
      841 catcacgact gtgcccgcgc cgcccatcta tgaccctggc ggcatctcgt cgctcgtcga
      901 cgatttcgca gtctcgtctc cggacagatg ggcgacggca ggtgtagccc ttctcatcga
      961 agcccacgtc gtttccgatc ctgatcgcga tgccttcatc caggagggga gcgccgtact
     1021 cgcggtagcg cctcatggac gttgagcgca cgaagggccg cctcgacggt gtcggctggg
     1081 ctcgagttga acgcgatggc actggtcggt gccgcgacgt gaaccaggtt gaccacgtac
     1141 tcgaagtact cgagcacggg ctctggcttg cacaacgcac gacggcgggc atgttcgcag
     1201 gcgggcgaaa cagcggctgc atcctggccg gggacggcgg ctgggtcgaa cccgatgagg
     1261 agcacacggg tcatacaagg gacgctagcc tgcgcctggc accgacaaga tgatgctctg
     1321 gaggcaatac accagagcga ggttccggcc tgcggcacgg gacgatggcg cgccatcgac
     1381 tggtcgacgc cgtgcgcgta tggtcgaacg ggacatctca tccgtctgcc gggcatcact
     1441 tcgatcgggc gtccgcggac tcctggcggg cacgaccgac agggggcccc atgccactgc
     1501 catggcgact agcaaccggc gtcacgctac ttcaaggtcg tacaaccacg atgcagtggt
     1561 ggtacaacca caccaacccc tacacgggcc atgtcgaggg ggactacagg ggcgcccaga
     1621 tcggcatcgc ctcacccgag cagctcttca cgtcctccgg ccgcgtgaac accgtcaacc
     1681 aaggctttga caacaccgga ggcaacgggt atcgctacta cgccgatgtc acgtgcgtaa
     1741 gtggtgggtc gggggcattc gacttctggg tccagccctt ccagtagagg agactcagca
     1801 tgcgacgtct ggttgtcttt gatcgcgacg ggaccatcgt ggccacagcg ccacaccccg
     1861 atgacatgcc accgcccgag ccgggcgcgc ccactttcct gggcttctct ccggcggagg
     1921 acgagcagca ggatgcgatc gacattcccg aagagctcag ttcgccggag gggatacgcg
     1981 aactgcatga gtcctatcgc gtcgagatga tcggcgatcg gccgacgctg acgcgccggt
     2041 aggccccgtg cggcaaacga tgcatgccgc aaagcgaccg ccggcttgtc agaaccccga
     2101 cgttcagacg acctccttcc gtggtcggtg aagggtcggc cggtagacga gctcctgggt
     2161 gcgtccgtcg aaggtgtggg attccagcag ctcgagatcg aagtcgccgg cgccgcccag
     2221 aatcgggctg gttccggtcc tgccggagac aacaggaaag atcgtcacct ggacacggtc
     2281 gaccagttcg gcggccatca gagcccagtt cagcgacagg ctggcctgcg aacgcagcgg
     2341 cacgcttgat tcctccttga gccggccgac gatgtccgcg gcgtcgccac tggcgacggt
     2401 cgcgtccggc cagccgaggg tgtcgcgcaa ggtggaggag atgacggtcg ccggcatgcg
     2461 gagcatcctc acgttccact catcctggtt gtgcgggtcg tcactccgca gcaagatctc
     2521 ggcgttctct cggaacgtgg tggctacgaa caccatccgc tgttcggaga ccaacaggga
     2581 ggcccggtgc tcgagcagct cagaccctgc ttgccccagt aaccacccca atctcccgca
     2641 gggccatatg agccgtagcc gtcgagggtg gagaagacgt cccaggtgta ggtcgcggtc
     2701 atgtcaggtc cttcctgcgg cccgatggtc ggtggccccg acgggccgca tcgctagggg
     2761 tatgccaacg acccttccac cgcgcggaca gcctctgaaa gtgggtcatc cgccacgacc
     2821 gcacatcggc agatccgcgt caagtcagcg gcgtgagcgt gcgtccagat tgtcgatctg
     2881 agccaaaagc ggtgggctca cacgctcgcg gcgagcactc ttgtcagcgg gaggagcgcc
     2941 gatggcaaag gacgtgcggt gcg
//
LOCUS       DATBFZ010000424         2886 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_4225833, whole genome shotgun sequence.
ACCESSION   DATBFZ010000424 DATBFZ010000000
VERSION     DATBFZ010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2886
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4225833"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1036)
                     /locus_tag="VH419_12815"
     CDS             complement(<1..1036)
                     /locus_tag="VH419_12815"
                     /inference="COORDINATES: protein motif:HMM:NF023275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-(1->3)-arabinofuranosyltransferase family
                     protein"
                     /protein_id="HEY7044545.1"
                     /translation="MTRLGVWTFIAFGSLLQQPFRTTFDTKFDLTSDPGQFLARSLHL
                     WNPHATFGELQNQAYGYLFPQGPYFLLADWASVPDWLAQRFWTALLLVLAYEGCRGLA
                     RAMGLNYPQAVLAGLVYAFSPRLLGGVGVLSGEILPSVLLPWMLWPLVSTLRGRTSVR
                     SGGLRAGCAVFAMSGVNAAGTAALLPAGLILIVWHWRHRYARRLLAWWTGAALLASLW
                     WLIPLALLGRYSPPFLDYIETSGATTFSTSWANSIRGADHWLGYVTLSGVPWWPGAHA
                     LFTEPVLVIAAGLVAGLGLWGLAQPSMPLRGVFMTIATLGLLCLVVGSPAATGSWIDG
                     PIRGLLDAGLA"
     gene            1144..1863
                     /locus_tag="VH419_12820"
     CDS             1144..1863
                     /locus_tag="VH419_12820"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY7044546.1"
                     /translation="MPSANKSADFSDIWPIADTIPGWLTMAQAGLLHEQARALHRGEL
                     AVEIGSHQGRSTVVLAHALRSRGSRLVAVDPFVDGRLFGGSPTKSKFETNVRAAGVQA
                     DVELREAYSTKLRPLWQEQLGMLYIDGKHDYWTVSDDLRWTRHVRPEGVVLIHDAFSS
                     IGVTLGLLHHVLLGSTLAYELREGSLALFRRRPPALRDRLRLLAQLPWWISNVVIKVL
                     LRLRLRPVARLLGHDSPYDPY"
     gene            complement(1886..2695)
                     /locus_tag="VH419_12825"
     CDS             complement(1886..2695)
                     /locus_tag="VH419_12825"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY7044547.1"
                     /translation="MSIASQVRLFVRRRLRFPVPDDALVLDVGSGDKPSWRADVLLDR
                     YVDESYAGQRSGTSRARVSRPLFDADAADMPFRDDAFAYTICSNLLEHVPDPAAVLEE
                     LMRVSSAGYIEVPEAASAKIVDFPSHVWWCRLDTTERPPTLVFSAKSDRHFDPEIGAY
                     LDRTGLDRRVARVLDSRFEDRVLMIDWRDKISYRIEGRVPDDFMTHALEQPGHGRTLS
                     ATAILAATRALTLPRRRRARHRIRFDDIVKDEFRKGTDELLTRRIYRVDSA"
BASE COUNT          539 a          933 c          956 g          458 t
ORIGIN      
        1 gcgcgaggcc cgcgtcgagc agcccgcgga tcggcccgtc gatccaggag ccggtggcgg
       61 ccggactgcc gacgaccaga cagaggagcc caagtgtcgc gatcgtcatg aagacaccac
      121 gcagtggcat agacggctgg gcgagccccc acaacccgag gccagcgacc aatccggccg
      181 cgatcacgag cactggctcg gtgaagagcg catgcgcgcc gggccaccag ggaactccgg
      241 agagggtgac gtaaccgagc cagtggtcgg cgccgcgaat gctgtttgcc cacgacgtcg
      301 agaatgtcgt cgcgcccgac gtctcgatgt aatccaggaa gggcggactg tagcgaccca
      361 ggagcgcgag cggaatcagc caccacaacg atgcgagcag cgccgccccg gtccaccagg
      421 ccagcaatcg acgcgcatac ctgtgccgcc agtgccagac gatcaggatc aggccggccg
      481 gaagcagtgc cgccgtaccg gctgcgttca cgccgctcat cgcgaagacg gcacagccgg
      541 cccgcagacc gccggagcgt acgctcgtcc tcccgcgcag cgtcgacacc agcggccaga
      601 gcatccaggg cagcagcacc gacggcaaga tctcgcctga gagaactccc accccgccga
      661 gcagacgcgg cgagaaggcg tacacaaggc ccgcgagaac cgcctgcgga tagttgaggc
      721 ccatggccct ggccaggcct cggcaaccct cgtaggcgag gacgaggaga agggcagtcc
      781 agaaccgctg agccagccag tccgggaccg acgcccagtc ggcgaggagg aaatagggtc
      841 cctgggggaa cagatagccg taggcctggt tctggagctc gccgaaggtg gcgtgcgggt
      901 tccacagatg caagctccgc gccaagaact gaccggggtc gctcgtcagg tcgaacttgg
      961 tgtcgaacgt cgtacgaaaa ggctgctgga gcagcgagcc gaacgcgatg aacgtccaga
     1021 ctcccagacg ggtcacgacc tcatgcgtga ccgggcgact ggtagcgatg gtgtccccca
     1081 attccggccc ctggagtagg gtgcgggcgc agtttagccg agcccgaact cgtgaggtgc
     1141 cctgtgccaa gtgccaacaa gtccgcagac ttcagcgaca tctggccgat cgcggacacc
     1201 attcccggtt ggctcaccat ggcccaggcc gggctactgc acgagcaagc ccgagcgctg
     1261 catcgaggtg agctcgccgt cgagatcgga agtcaccagg ggcgttccac ggtggtcctc
     1321 gcgcatgcat tgagatcgcg aggatcccgg ttggtggcgg tcgatccgtt cgtggatggg
     1381 cggctgttcg gcgggtcacc gaccaaatcg aagttcgaga cgaatgtccg tgcggcggga
     1441 gtccaggcag acgtcgaact gcgagaggcg tactcaacca aactccgccc gttgtggcag
     1501 gagcaactcg gcatgctcta tatcgacgga aagcatgact actggaccgt gagtgacgac
     1561 ctccgctgga cacgacacgt gcgtcccgag ggcgtcgtgc tcatccacga cgcgttctcc
     1621 tccatcggcg tgaccctggg tctgttgcac cacgtgctcc tcggctcgac gctcgcatac
     1681 gagctgcggg agggctctct cgcactgttt cggcgccgcc ccccggcgtt gcgggaccga
     1741 ctgcgtctcc tcgcccagct gccgtggtgg atctcgaatg tggtgatcaa ggtcctgctg
     1801 cgactgcggt tgcgaccggt cgcccgcttg ctcggccacg actcgccgta cgacccctac
     1861 taactgccgt gcggtgtggg cgacttcagg cgctatctac ccggtagatg cgacgggtca
     1921 acaactcatc ggtacctttt cgaaactcgt ccttgacgat gtcgtcgaag cggatccgat
     1981 gcctggctcg gcggcgtcga ggcagcgtca gcgcgcgagt agcggcgagg atcgcggttg
     2041 cgctgagcgt cctgccgtgt ccgggttgtt ccagcgcgtg agtcatgaag tcgtcgggaa
     2101 cccgaccctc gatgcggtat gagatcttgt cccgccagtc gatcatgagc acgcggtcct
     2161 cgaaccggct gtcgagcacc cttgccaccc gacggtcgag tccggtccga tccaggtagg
     2221 cgccgatctc gggatcgaag tgccgatcgc tcttcgcgct gaacaccaac gtcggcggtc
     2281 tttcggtcgt gtcgagccgg caccaccaga catgactggg aaagtcgacg atcttcgcac
     2341 tcgcggcctc cggcacctcg atgtaccccg ccgaggagac ccgcatcaac tcttccagca
     2401 ccgccgccgg atccggcacg tgctcgagca ggttggagca gatggtgtaa gcgaaggcgt
     2461 cgtcacggaa gggcatgtcg gctgcgtccg cgtcgaagag tggtcgagag acgcgtgctc
     2521 gacttgtgcc ggagcgttga ccggcgtagc tctcgtcgac atagcgatcc agcaggacgt
     2581 cggctcgcca ggatggtttg tcaccactcc cgacatcgag gaccaaggcg tcgtcaggca
     2641 ccgggaaccg gagtcgacgc cgaacgaaca gccggacctg gctcgcgatg gacacggcca
     2701 cagtgtggca ccgccaactc gatcgtgaac gacgcgcgag cgcgtatcga acctgcaaca
     2761 agttccaatt cgtgtcgctc cagacctccc ggttactcgc gagttgcgct acggtgctcc
     2821 ggatcgttgc agccttggga ggggacgttc gtgaagcgca aggtcattgg gctggtcctg
     2881 tgtggg
//
LOCUS       DATBFZ010000425         1921 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_4415025, whole genome shotgun sequence.
ACCESSION   DATBFZ010000425 DATBFZ010000000
VERSION     DATBFZ010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4415025"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            135..569
                     /locus_tag="VH419_12830"
     CDS             135..569
                     /locus_tag="VH419_12830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013861379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY7044548.1"
                     /translation="MTKWGLTVDCAEPAELAEFWTLALGYEQASPPEGFASWHEWLDR
                     LDVPEEEWDDAAYLNDPAGAGPSLSFLKVPEGKIVKNRLHIDLKVGGGRQTPWDVRWP
                     KVLDMVERLTAAGGTVQRIDEINGQGDHVVMEDPEGNEFCVL"
     gene            613..1044
                     /locus_tag="VH419_12835"
     CDS             613..1044
                     /locus_tag="VH419_12835"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY7044549.1"
                     /translation="MRRYVIEKTARSSADPGEVYRLLRDGATWPEWSAIKGFELEREG
                     DGEPEGVGAVRVFVNGPIRGRDEVTGFAKDRQFRYNHLDGLPVRDYRGVVDLVPKDTG
                     TEITWTVSFGPKYPGTGWVLQRAFGRFVQQCVDGLAKHAAA"
     gene            1336..>1921
                     /locus_tag="VH419_12840"
     CDS             1336..>1921
                     /locus_tag="VH419_12840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY7044550.1"
                     /translation="MTDLSGWLRQGVDAVEAWQRSFADYPAHPCQQVDPDRFRDAFAE
                     LTGRLTDNYPFFHPDYAGQMLKPPHPVAVAGYLTAMLVNPNNHAQDGGPATAKMEREA
                     VAALATMFGFDAHLGHLTGGGTVANLEALFVARESHPGKGVAFSAESHYTHARMCHVL
                     GMRGHTVPVDAAGRMDLGALEHLLDKGDIGTVVVT"
BASE COUNT          300 a          615 c          714 g          292 t
ORIGIN      
        1 ggcgcacgag ttcgcccggg aggtgcccgc cgccgagctg gtcgtgaccc tcgccgcgga
       61 tgccaggacg gcgcttgccc tggccacgcg gcggttttcc tgaattgtca gaccccgcgc
      121 gtagcgtccc gggcatgacg aaatggggac tgaccgtcga ctgtgccgaa ccggccgagc
      181 tggccgagtt ctggacgctg gcactggggt acgagcaggc gtcgccgccc gagggcttcg
      241 cgagctggca cgagtggctc gacaggctcg acgtgccgga ggaggagtgg gacgacgccg
      301 cgtacctgaa cgatccggcc ggggcgggcc cgtcgttgtc gtttctcaag gttcccgaag
      361 gcaagatcgt gaagaaccga ttacacatcg acctcaaggt cggcggtggc cggcagacac
      421 cgtgggacgt gcggtggccg aaggtgctcg acatggtcga gcggttgacg gctgccggcg
      481 ggacggtcca gcgcatcgac gagatcaacg gtcagggtga ccatgtggtg atggaggatc
      541 cggagggcaa cgagttctgc gtgctgtgac atttgggtcg cgcggggtcg gaaatggctt
      601 agtatcacgg ccgtgcgccg ctacgtgatc gagaagaccg cacggagctc cgcggacccg
      661 ggcgaggtat atcgcctgct gcgagacggt gccacgtggc cggagtggtc ggcgatcaag
      721 gggttcgagc tggagcggga aggcgacggc gagccggagg gcgtcggcgc ggtgcgcgtg
      781 ttcgtcaacg gcccgatccg cgggcgcgac gaggtcacgg gattcgcgaa ggaccggcag
      841 ttcaggtaca accacctgga cggcctgccc gttcgcgact accgcggcgt ggtcgacctg
      901 gtcccgaagg acacgggcac ggagatcacg tggacggtca gcttcgggcc caagtacccg
      961 ggcacgggct gggtgttgca gcgcgccttc ggccggttcg ttcagcagtg cgtggacggc
     1021 ctggccaagc acgcagccgc ctgacggccg cgtcgccagc cggcgattcg cgccccgggt
     1081 cggtccctgg acatactcag agtttgcttg ctgtgcaatg aaacccgcct caggcgtcac
     1141 gcgatgaagt ggtgaaacac gccggtccgg tgacgagcgc gcgactgccc acccgggcaa
     1201 cgtcgtcgcg cgcttgtgct ggtggtgcgg ggacggggcg cccgctgacg cacgacggtc
     1261 actgccgaag gactctctcg gagcgggagg cgtcctccgt cgcgttcggg ccggaggcat
     1321 gcgattgtga ggggcatgac cgatctttcc gggtggctgc gccagggcgt cgacgccgtc
     1381 gaggcgtggc agcggagctt cgccgactat ccggcgcacc cgtgccagca ggtcgatccc
     1441 gaccggttcc gggacgcgtt cgccgagttg accggccggc tcacggacaa ctacccgttc
     1501 ttccaccccg actatgccgg ccagatgctc aagccgccgc atccggtggc cgtcgcgggc
     1561 tacctcaccg cgatgctcgt caacccgaac aaccacgctc aggacggcgg accggccacc
     1621 gcgaagatgg agcgggaggc cgtcgccgcg ctggcgacca tgttcggctt cgacgcgcat
     1681 ctcgggcacc tcacgggtgg cggcacggtc gccaacctcg aggcgttgtt cgtcgcgcgg
     1741 gagtcgcatc cgggcaaggg cgtcgcgttc agcgcggaga gtcactacac gcatgcgcgc
     1801 atgtgccacg tgctcggcat gcgcggccac acggtcccgg tcgacgccgc cgggcggatg
     1861 gacctcggcg cgctcgagca cctgctggac aaaggggaca tcggcacggt cgtcgtcacc
     1921 g
//
LOCUS       DATBFZ010000426         6234 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_2267676, whole genome shotgun sequence.
ACCESSION   DATBFZ010000426 DATBFZ010000000
VERSION     DATBFZ010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 6234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6234
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2267676"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            219..686
                     /gene="bcp"
                     /locus_tag="VH419_12845"
     CDS             219..686
                     /gene="bcp"
                     /locus_tag="VH419_12845"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020574015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HEY7044551.1"
                     /translation="MSRLEVGDKAPDFTLSDDTGEQVSLAGFRGRKVILYVYPAAMTA
                     GCTTQACDFRDSLEPLSAAGYVVLGLSPDGPEKLAEFRAHDKLTFPLLSDPDASVLRA
                     YGAYGEKKLYGKTVVGVIRSTFVIDEEGVVEKAMYNVKATGHVAKLRRELAIA"
     gene            725..798
                     /locus_tag="VH419_12850"
     tRNA            725..798
                     /locus_tag="VH419_12850"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:759..761,aa:Leu,seq:tag)
     gene            complement(856..1467)
                     /gene="rdgB"
                     /locus_tag="VH419_12855"
     CDS             complement(856..1467)
                     /gene="rdgB"
                     /locus_tag="VH419_12855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012919498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HEY7044552.1"
                     /translation="MSRVFLASRNPKKLEELRRILASVIPDVHVLGLDDVTPYDEPVE
                     TEPSFEGNALLKARAAVAATGLPSLADDSGLCVDALNGMPGVLSARWAGAAKDDAANN
                     ALLLDQLSDVPDERRGAHFRCAVAFCLPDGDDAVVFGEMHGSVIREMRGSGGFGYDVV
                     FVPDGETRTSAEMPAAEKDAISHRGRSLRAVAPLVAAALDSSG"
     gene            complement(1464..2183)
                     /gene="rph"
                     /locus_tag="VH419_12860"
     CDS             complement(1464..2183)
                     /gene="rph"
                     /locus_tag="VH419_12860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012919497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEY7044553.1"
                     /translation="MTRADGRTPDELRPIAITRNWLDHAAGSVLIEFGRTRVLCAASA
                     AVGVPRWRKDSGLGWVTAEYAMLPSSTHTRSDRESVKGRIGGRTHEISRLIGRSLRGI
                     IDYKALGENTIVLDCDVLQADGGTRTAAITGAYVALADAVTHLRGEGALAGEPLTGSV
                     AAVSVGIIDGEPRLDLPYEEDVRAETDMNVVMNGDGAFVEVQGTAEAKAFDRAMLNSL
                     LDLAAKGCAELSRLQNEALAA"
     gene            2313..2813
                     /locus_tag="VH419_12865"
     CDS             2313..2813
                     /locus_tag="VH419_12865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009085696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY7044554.1"
                     /translation="MRVYDDALPVVYGYFVTRCGEPAVAEDLTAQTFLSAVEAVRRTK
                     PPVIDVKWLIGVARHKLADHWRAAARDRARVAAVENEQALAPGREDPWEGSLDRLLAA
                     QTLRQLAPQHRLVLTLRYVDDLPVAEVAETIGRSVHATEGVLVRARSSFRRHYPRPRD
                     DEGGSR"
     gene            2810..3379
                     /locus_tag="VH419_12870"
     CDS             2810..3379
                     /locus_tag="VH419_12870"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY7044555.1"
                     /translation="MTGTPADPFSVLATPLVDVSPDPAFAHNLRGRLERALRLPEGVS
                     MSDLEDVDEAAQDTAAPRPAAIPYLAASPARAAIDWYVDVLGARLVGDPIVMPDGRIG
                     HSELAIDDGVLYLSDAHPELGVVAPTVGQHSVSLMLRVDDVDETLARAESAGARRDRE
                     PYDGYGQRNAWMIDPFGHRWGLWGPVTRA"
     gene            complement(3421..4182)
                     /locus_tag="VH419_12875"
     CDS             complement(3421..4182)
                     /locus_tag="VH419_12875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020574005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY7044556.1"
                     /translation="MRLTIIGCSGSMPGPESAASCYLVEAPYAGRTFRLVLDLGSGAL
                     GTLQRFVALDTVDAVALSHLHADHCLDLCGYYVFRKYHPGGPLPHVTVYGPEGTAQRM
                     ARAYDLPDEPGMSDVFSFVPYADSTMEIGPFSVDVARVHHPVPAYAVRVSHDGRSLVY
                     TGDTGPCSSLDDLAKGCDLLLAESSFVEGGRHNPMDMHLTGRQAALTAERAGVGQLVL
                     THIPPWHDREVVRSEAVPHFAGPVSLAVTGASYDL"
     gene            complement(4179..4985)
                     /gene="murI"
                     /locus_tag="VH419_12880"
     CDS             complement(4179..4985)
                     /gene="murI"
                     /locus_tag="VH419_12880"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020387169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEY7044557.1"
                     /translation="MVDAPIGIFDSGFGGLTVARAVLDQLPYEPVLYLGDTARQPYGP
                     QPIGDVREFALECLDHLVDRGVKALVIACNSASAAVLRDARERYPLPVVEVIFPATRR
                     AVAATRSGHVGVICTEATKQSMAYDDAFAAAPHVTLTTQACPRFVEFVEAGVTSGAEL
                     IEVAHQYLDPLRGAGVDTLILGCTHYPLLTGVISYVLGDDVTLVSSAEETAKDVYALL
                     LRDGLQRDPGLPGPRHHFLTTGRPSEFREIGRRFLGPELDLVDQFAWVGV"
     gene            complement(5131..6132)
                     /locus_tag="VH419_12885"
     CDS             complement(5131..6132)
                     /locus_tag="VH419_12885"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020390351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0002189 - ribose phosphate
                     diphosphokinase complex [Evidence IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEY7044558.1"
                     /translation="MREIVVFSGSAHRQLARSISTSLGVRPSPVEIKRFSNDCLQVQL
                     QANCRQRDVYLVQPLVPPTQEHLMELLLMIDAAKGASAAQVTAVIPHYAYARSDKKDA
                     SRISLGGRLVADMLVTAGVDRILTMTLHAPQVHGFFSVPVDHLTALGVLADHFRDRDL
                     TDTVVVSPDLGNAKTATQFARLLGLPVAAGSKQRLSDDSVVIDAIVGDVGNKRAIVLD
                     DEIANGGSIVELLDKLHDLGCTEAAVACTHGLFVGKAVERLRGHPAISEVVTTDTVPA
                     PSAWPELKVRSVAQLFAEAISRVHAGESVSSLFDGVDPTHGPPQPTLPFDAETLETV"
BASE COUNT          999 a         2162 c         2143 g          930 t
ORIGIN      
        1 gccggcaagc cggttgcggg tttcggcgat ctcagtcgcg atctcgtcgg gggtccgggt
       61 caccgaactc acgtgtcctc ctgccgtcgg cgcagctgcg gtgtctgacg gacgtcaagt
      121 cttgcacgac ggcaccaccg acgctgtccc ggtcggtgcg ccttcggtcg ccgccaggcg
      181 tgcggtaggt tgccagagcc gatcacagga ggtgcgggat gagccggctc gaggtgggtg
      241 acaaggcccc cgacttcacc ttgtccgacg acacgggaga gcaggtcagc ctcgccggct
      301 tccgcgggcg caaggtgatc ctctacgtct acccggccgc gatgaccgcc gggtgcacca
      361 cacaggcctg cgacttccgc gactctctcg agccgctgtc cgctgcaggt tacgtcgtgt
      421 tgggactttc gccggacggg cccgagaagc tcgcggagtt ccgtgcgcac gacaagctga
      481 ccttcccgct gctctccgat ccggacgcgt cagtgctgag ggcgtacggc gcgtacggcg
      541 agaagaagct gtacggcaag accgtcgtcg gcgtgatccg ttccaccttc gtcatcgacg
      601 aggagggcgt ggtcgagaag gcgatgtaca acgtcaaggc caccggtcat gtcgccaagc
      661 tccgccgcga gctggccatc gcctgaccgt ccctcgctgc gtgttttcgt aggatgacgc
      721 tcgaagggcc gtagcccaac aggcagaggc acacggttta ggtccgtgcc agtgtgggtt
      781 cgactcccac cggccctacc gacggtgcgg tttggcgttt cctccgacca cgcgcgcaaa
      841 catgctggac gcgtctcagc cggacgaatc gagggcggcg gcgacgagcg gagcgacggc
      901 gcgcagcgac ctgccacggt gcgagatcgc gtccttctcc gctgccggca tctctgcgct
      961 cgtccgggtc tcgccgtcgg gaacgaagac gacgtcgtat ccgaacccgc cgctgccccg
     1021 catctcacgg atgaccgagc catgcatctc cccgaagacg acggcgtcgt ccccatccgg
     1081 gagacagaac gcgactgcac accggaagtg tgcgccgcga cgctcgtcgg gtacgtcgga
     1141 cagctggtcg aggagcagcg cgttgttcgc cgcatcgtcc ttcgctgcac ccgcccagcg
     1201 cgcggagagc acgccaggca tgccgttcag cgcgtccacg cacagcccac tgtcgtcggc
     1261 aagggacggc agcccggtcg ccgccacggc ggcacgggcc ttgaggaggg cgttcccctc
     1321 gaagctcggc tcggtctcca ccggctcgtc gtacggcgtg acgtcgtcca gcccgagcac
     1381 gtggacgtca gggatcaccg aggcaaggat gcgccgcagc tcttccagct tcttcgggtt
     1441 gcgggaagcg aggaagaccc ggctcacgcg gcaagcgcct cgttctgcag gcgcgacaac
     1501 tcggcgcagc ctttcgcggc gaggtcgaga agcgagttga gcatggctcg gtcgaatgcc
     1561 ttcgcctcgg ccgttccctg cacctcgacg aacgcgccat cgccgttcat cacgacgttc
     1621 atgtcggtct cggcacggac gtcctcctcg tacggcaggt cgagccgcgg ctccccgtcg
     1681 atgatgccga cggagactgc cgccaccgac ccggtcagcg gctcccccgc caacgcgccc
     1741 tcaccccgca gatgggtcac ggcgtcggcc agagcgacgt acgcaccggt gatcgcggcc
     1801 gtccgcgtgc cgccgtcggc ctgcaggacg tcacagtcga gcacgatggt gttctcgccc
     1861 agcgccttgt agtcgatgat cccgcgtagc gacctcccga tcagccgcga gatctcgtgc
     1921 gtgcggccac cgatccgccc cttcaccgac tcgcggtcgg accgcgtgtg ggtcgagctc
     1981 ggcagcatgg cgtactccgc cgtcacccaa ccgagtccgc tgtccttgcg ccatcggggc
     2041 acgccgaccg cggcgctcgc ggcgcacaac acgcgcgtcc tgccgaactc gatcagcacc
     2101 gaaccagcgg cgtggtcgag ccagttccgc gtgatcgcga tcgggcgtag ttcgtcgggg
     2161 gtgcggccgt cagctcgggt catgcgccga ccatatccgc cacgacccac gagaaagctg
     2221 gaccaggacc gtcggagttc gcccgctcgc aacgatgatg aggtgtgaca cgtccaccgt
     2281 cagggacggc gagacagcct gagcaggcgc tgatgcgcgt gtacgacgac gccctcccgg
     2341 tcgtctacgg ctatttcgtg acgcgctgcg gcgaacccgc tgtcgccgag gacctcacgg
     2401 cgcagacgtt cctttccgcc gtggaggcgg tccggcgtac caagcctccg gtgatcgacg
     2461 tcaagtggct catcggagtc gcccggcaca agctcgccga tcactggcga gccgccgcgc
     2521 gcgaccgcgc cagggtcgcc gctgtcgaga acgagcaggc gctcgcgccc gggcgtgagg
     2581 atccctggga aggaagcctc gaccggctcc tcgccgcgca gaccctgcga cagcttgccc
     2641 cgcagcatcg cctcgtgctc acgctgcgct acgtggacga cctgccggtc gccgaggtgg
     2701 ccgagacgat cggccgcagc gtacacgcga cggagggcgt gctggtgcgc gcgcggtcga
     2761 gctttcgacg gcactacccg aggccgcgag acgacgaagg agggtcacga tgaccggcac
     2821 acctgccgac cccttcagcg tgctcgcgac gccgctcgtc gacgtctcgc ccgatccggc
     2881 gtttgcccac aacctgcgcg gacggctcga acgagccctg cgcctacctg aaggagtctc
     2941 catgtccgac ctggaagatg tcgacgaagc agcgcaagac accgcggccc cccgtccggc
     3001 ggcgatcccc tacctcgccg ccagtccggc tcgcgccgcc atcgactggt acgtcgacgt
     3061 gctcggtgcc cgtctggtcg gagatccgat cgtgatgccg gacggacgga tcggccacag
     3121 cgagctggcg atcgacgacg gcgtcttgta cctctccgac gcccatcccg agctcggggt
     3181 cgtcgcaccg accgtcggcc agcacagtgt cagcctgatg ctgcgcgtcg acgacgtgga
     3241 cgagaccctc gcccgggcag agtccgccgg tgcgcgacgc gaccgggagc cctacgacgg
     3301 ctacggccag cgcaacgcct ggatgatcga cccgttcggg caccggtggg gcctctgggg
     3361 gccggtgacc cgcgcctgag gccacgacag acggcggcgg acgacgcaga ccgcggccag
     3421 tcagaggtcg tacgacgccc cggtgaccgc caaggacacc ggcccggcga agtgcggaac
     3481 cgcctcggat cgtacgacct cccggtcgtg ccagggcggg atgtgggtga ggacgagctg
     3541 accgacgccc gcccgctccg ctgtgagcgc cgcctgtctg ccggtcagat gcatgtccat
     3601 ggggttgtga cgaccgccct cgacgaacga cgactctgca agcaagaggt cacatccctt
     3661 ggcgagatcg tccagcgacg agcagggtcc ggtgtccccg gtgtagacca gcgaccggcc
     3721 gtcgtggctg acgcggacgg cgtacgccgg aaccgggtgg tgaactcgcg ccacatcgac
     3781 gctgaacggg ccgatctcca tcgtcgagtc cgcgtacggc acgaacgaga agacgtcgct
     3841 catgcccggt tcgtcgggca gatcgtaggc acgggccatc cgctgcgcgg tgccttcggg
     3901 accgtagacg gtgacatgcg gcagcggacc gcccgggtgg tacttgcgga acacgtagta
     3961 gccgcacagg tccagacagt ggtcggcgtg caggtgcgac agcgccaccg catcgacggt
     4021 gtcgagagcg acgaaccgtt gcaatgtccc gagcgcgccg ctgcccaggt cgagcacgag
     4081 ccggaacgtc cgcccggcgt acggcgcctc gacgagatag caggaggcag ccgactccgg
     4141 gccgggcatc gaacccgagc aaccgatgat cgtcagtctc atacgccgac ccacgcgaac
     4201 tggtcgacga gatcgagctc gggtccgagg aaccgcctgc cgatctccct gaactcgctc
     4261 ggccgaccgg tcgtcaggaa gtggtgtcgg ggccccggaa gcccggggtc gcgctgcaag
     4321 ccgtcccgca gcagcagcgc gtagacgtcc ttggcggtct cctcggcgct cgacaccaac
     4381 gtgacgtcgt caccgaggac gtacgagatc accccggtca gcagcggata gtgcgtgcaa
     4441 cccaggatca gcgtgtccac accggcaccc cgcagcgggt ccaggtactg atgggccacc
     4501 tcgatcagct ccgcgccact cgtcacgccg gcctcgacga actcgacgaa gcgaggacat
     4561 gcctgcgtgg tcaaggtgac gtgcggcgcc gcggcgaacg cgtcgtcata cgccatcgac
     4621 tgcttggtgg cctcggtgca gatgacgccg acgtgaccgg accgggtcgc tgcgacggcc
     4681 cggcgagtgg cgggaaagat cacctcgacc accggcaggg ggtagcgctc ccgagcgtct
     4741 cgcagtacgg ccgcgctggc ggagttgcag gcgatgacga gcgccttcac gccgcggtcg
     4801 acaaggtggt ccagacactc gagcgcgaac tcgcggacgt caccgatcgg ctgcggcccg
     4861 tagggctgcc gggcggtgtc ccccaggtac agcaccggct cgtacggcag ctggtcgagc
     4921 acggcccggg ccacggtcag gccaccgaag ccggagtcga agatgccgat cggagcgtcc
     4981 accacggatg tgagcgtacg acggctgggg cgcctggcga ggaaactgcg accgacgcca
     5041 catgacgccg gcctcgtccg ccgcttcgtc gcgtccgtcc agctgaccgc ttgagcagcc
     5101 gcttcgacgg acgtaccggg cgaggagccc tcagacggtc tcgagcgtct cggcgtcgaa
     5161 cggcagggtc ggctgcggcg ggccgtgcgt ggggtcgacg ccgtcgaaca acgagctcac
     5221 cgactcgccg gcatggaccc ggctgatcgc ctcggcgaac agctgggcca ccgatcgaac
     5281 cttcagttct ggccaggcgc tcggcgcggg gacggtgtcg gtggtgacca cctcactgat
     5341 ggccgggtgg ccgcggaggc gctcgaccgc cttgcccacg aacagcccgt gggtacaggc
     5401 gacggccgcc tcggtgcagc cgaggtcgtg cagcttgtcg agcagctcga cgatcgagcc
     5461 gccgttggcg atctcgtcgt cgaggacgat ggctctcttg ttgcccacgt caccgacgat
     5521 cgcgtcgatc acgacggagt cgtcgctcag ccgctgtttg ctcccggcgg cgaccggcag
     5581 gcccagcagg cgcgcgaact gggtcgcggt cttcgcgttg cccaggtcgg gagagaccac
     5641 cacggtgtcg gtaaggtccc ggtcgcggaa gtggtcggcg aggacgccga gcgcggtgag
     5701 gtgatcgacc ggcacggaga agaacccgtg cacctgcggc gcgtgcaggg tcatcgtgag
     5761 gatgcggtcc acgcctgcgg tcaccagcat gtcggcgacg agtcggccac ccagcgagat
     5821 ccgggaggcg tccttcttgt ccgaccgcgc gtaggcgtag tgcgggatga cggcggtgac
     5881 ctgcgcggcg gacgcgccct tggccgcgtc gatcatcagc agcagctcca tcaggtgctc
     5941 ctgcgtgggt ggtaccagcg gttgcaccag atagacgtcg cgctgtcggc agttcgcctg
     6001 gagctgcacc tggaggcagt cgttgctgaa ccgcttgatc tcgaccggcg agggccggac
     6061 gccgagagac gtgctgatcg aacgggcgag ctgacggtgg gcacttccgc tgaagacgac
     6121 gatttcgcgc acgacgggcc tcgaggggtg agtgacctgc aatcgcggct cagcctacct
     6181 ccccctcctg aggcccctgc gaactgcggg acatgcacgt ttcgaacggt tccg
//
LOCUS       DATBFZ010000427        15247 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_1610573, whole genome shotgun sequence.
ACCESSION   DATBFZ010000427 DATBFZ010000000
VERSION     DATBFZ010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 15247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15247
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1610573"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="VH419_12890"
     CDS             complement(<1..234)
                     /locus_tag="VH419_12890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044559.1"
                     /translation="MTTTWRGHPWRSPRRLAKRGRRAGPLAAGRLFRFLVTTVMLLDG
                     VVTTAHERAMRVSTSRPPAGAGAAAVPSTGGVGG"
     gene            complement(440..2419)
                     /locus_tag="VH419_12895"
     CDS             complement(440..2419)
                     /locus_tag="VH419_12895"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY7044560.1"
                     /translation="MIRQPRWIVAVLVSLLTVSLSAPDGAAAQHRTAPVAVPIPDVPA
                     GEPKADRTVSDRSCHHGEARCGHVRRLLDPNDPAYDHIGIAYELYHRRDRSRPGLGTI
                     VAVEGGPGYSTTDSRSYYLDLFGPLLDRRRLLLVDNRGTGKSSPVRCPWLQSYRGNRN
                     LAIGRCGHRLGATSDVYGTAFATDDLAAVLDRFAIRKVDLYGDSYGTFFSQTFALRHG
                     DRLRTLTLDAAYFVGGKDPWYSDTNRALRHAFTVACDRSPACAHHPGTPMHRLARLAQ
                     LLRRHPISGDAPNADGVTRHIRVTVNNLIDMVTGAATTPTLYRELDAAARAVLRKHPY
                     TQPLLRLARETVYVGGAGPVDEYSEGLYVAVACNDYPQPYDVTAPPKTRYRQFARSIT
                     RLQRDRPGVFAPFRVREWVSSPYGYYDDCLRWPAPSRWVHPVPPHAAYPDVPTLVLGG
                     DLDSLTSPEGARDTANAFPNSTFVETANTGHVSALGDFDVCASLIVRRFVRHRDPGDT
                     SCARRYHENRLVDRFVRTAAGTGWHGERYRTARVAAATAADVMARWLSMYGYNGVGLQ
                     GGTFTTRGGSYTAPHPVVTWDLDGVKWVRDVAVSGRMRWRQHSGLVVASVRTTGTGAL
                     RAHLRLRWNDFDRHARAIARGTVGGRRVAFEFPAP"
     gene            complement(2586..3545)
                     /locus_tag="VH419_12900"
     CDS             complement(2586..3545)
                     /locus_tag="VH419_12900"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEY7044561.1"
                     /translation="MTRFGYFLSCEEYSPAELIEQATRAEQAGFDGLWISDHYHPWND
                     EQGESPFVWSMIGALSQACSLPVTTAVTCPTIRTHPAVIAQAAATSAVLLDGRFRLGV
                     GSGEALNEHVLGTAWPTADVRLEMLEEAVEVMRELWSGDVVTHRGRHYTVDTARIYTL
                     PEEPPPVYVSGFGPKAVDVAGRIGDGYVTTSPDDTLVKRFRDQAGADKPVQGGFKVCY
                     AETPDEGAKIAHRLWANSGLPGELAQVLPSPRHFEQASSLVTEEMTRSSVVCGNDASE
                     HVKAFSPYLDTGFDEIYVANMGPHFADMLSMYADEVLPALRNR"
     gene            complement(3598..5364)
                     /locus_tag="VH419_12905"
     CDS             complement(3598..5364)
                     /locus_tag="VH419_12905"
                     /inference="COORDINATES: protein motif:HMM:NF012239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SelB C-terminal domain-containing protein"
                     /protein_id="HEY7044562.1"
                     /translation="MHVVATAGHVDHGKSTLIEALTGQQPDRLAEERRRGLSIELGYC
                     WTGLPRSGDVAFVDVPGHERFISTMLAGVGPVPVVLFVVAADDPWMPQADEHLAILDA
                     LEVNTGVLAVTRSDLGDAEAALRKARAATAATTLADIPAVIVSGVTGEGLSELRTTLD
                     RVLASAPPPDPAADVRLWVDRMFTRPGAGTVVTGTLPAGRIRTGDTLACDSDLIHVRG
                     LQSMTKDIDQATGVARVSLRLGSGVPTGLRRGTALTTPGAWYTSDLLDVSVTGPGTPP
                     AEPVLHVGSASVSARVRPLATTRDGWLVRVRVARPLPWHVGDVALLRDPGRRLVWRCR
                     VLDPAPPGLERRGDGRRRADALAAASGTPDLDEELRRRGMAATGLLRRLGVPRDQLSS
                     ATDGRSAASGWLLDPARLSELSQRLVAAVAAQTAHDPLEDGLTPAAAARLLELPDPGL
                     ALTVVRPPLAYVDGRIRRQSTALPGALEAALSQLEDQFRLHPFQAPDADRLKELGLDR
                     RSLAAAERAGRVLRVGETVVLLPGADQQAVECLRTLPQPFTTSAARQKLDTSRRVVLP
                     LLAHLDRAGLTRRLADDRRTVI"
     gene            complement(5367..6674)
                     /gene="selA"
                     /locus_tag="VH419_12910"
     CDS             complement(5367..6674)
                     /gene="selA"
                     /locus_tag="VH419_12910"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009080036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HEY7044563.1"
                     /translation="MDDPRRAIPATDMLLADPVLAEACDRLGRRTVLDAIRRAQAAAR
                     DGRLPATAVRDQAVRLLPSAPTSLRRVVNATGVLVHTNLGRAALSDAAVSALEVAARS
                     TDVELDLASGRRGRRGRSTLAALATSVPAAEDVHVVNNGAAALALVASALAHGRSIVV
                     ARGELVEIGDGFRIPELLESTGARLREVGTTNRVGLGDYADAIGDDTAFVLKVHPSNF
                     VVSGFTSSVPVRSLSALPVPVVADIGSGLLHPHPRLPDEPDATTMLEAGAALVTASGD
                     KLLGGPQCGLLMGRADLVGRVRRHPLARAYRVDKLTLAALEATLSGPPGPVSRALVTE
                     QAALRDRATALAGKLEPHVHAEVVETLAAIGGGGAPGVDLPSAAIALPTGLAVPLRLG
                     DPAVLGRVHHDRLLLDLIAVDPRDDTILVEQVRRAAKESTEVD"
     gene            complement(6708..6802)
                     /locus_tag="VH419_12915"
     tRNA            complement(6708..6802)
                     /locus_tag="VH419_12915"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6766..6768),aa:Sec,seq:tca)
     gene            6878..10102
                     /gene="fdh"
                     /locus_tag="VH419_12920"
     CDS             6878..10102
                     /gene="fdh"
                     /locus_tag="VH419_12920"
                     /EC_number="1.17.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013476752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:7448..7450,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase"
                     /protein_id="HEY7044564.1"
                     /translation="MTTTEQTFLPWPAVRQLLGPDRLGRGTAAQSARSRTLRPRIADA
                     DRVVDSICPYCAVGCGQQVYVKDERVIQIEGDPNSPISRGRLCPKGSASYQLVTSPQR
                     VTKVRYRRPRGTEWEDLDLDVAMDMIADRVIASRREYWQEYSDDGVRLRRTLGISSLG
                     GATLDNEENYLMKKLYTAMGAIQIENQARIUHSSTVPGLGASFGRGGATTFQQDLANA
                     DCILIQGSNMAENHPVGFQWVMEAKARGATIIHVDPRFTRTSALADVHATLRAGTDIV
                     FLGALVNHVLVNEADFREYVVAYTNAAAILSDDYEDPEDLSGVFSGFDEDQGRYDPTT
                     WEYERARRKEEAGGEGSEEAHAAHEASRSEAAGSGGPTVGAKADRDETLQHPRCVYQV
                     LRRHFARYTPDMVEDVCGVPREQFVRIAEALERNSGRDRTSAICYAVGWTQHSTGVQM
                     IRTAAILQLLLGNIGRPGGGIMALRGHASIQGSTDIPTLYNILPGYLPMPHADQHQSM
                     EDYVHVDEEAAGFWGNIRTYLVSILKAYFGDAATTDNEFCFDYLPRITGDHGTYRIVK
                     DQVERGGGGYFLVGENPAVGSSNGKMQRLGLANLDWLVVRDMVMVESATFWKDGPEIE
                     TGELRPEDIGTEIFFLPAAAHTEKDGTFTNTQRLLQWHHKAVEPPDDATSDLWFYYHL
                     GKRLKAKLADSTDPVDAPLQDLVWDYPEEGPRAEPSADAVLREINGTGPDGAALSAYT
                     ELKADGSTSCGCWIYCGVYADEVNQSARRKPGSEQSWVAPEWGWAWPANRRVLYNRAS
                     ADPDGDPWSERKAYVWWDSDAGKWTGHDNPDFIPDREPHHVPEEGATGPDAIGGSDPF
                     IMQTDGKGWLFAPKGLADGPLPTHYEPVESPIRNPAYRQQSNPVAVAYETAVNPVHPE
                     GAEVFPYVFTTYRLTEHHTAGGMSRTLPYLAELQPEFFCEVSPALAAERGLTHAGWAT
                     IVTARTAVEARVLVTDRMRRLRIGDQWVHQIGLPYHWGGNGLTRGDSANDLVNVTVDP
                     NVRIQDKVGTCDIRPGRRPRGAALLSFVADYRRRAGMTEDGEQR"
     gene            10099..11013
                     /locus_tag="VH419_12925"
     CDS             10099..11013
                     /locus_tag="VH419_12925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013861430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEY7044565.1"
                     /translation="MSWSGPLRDVAGDAGHVDHPPRMGFFTDTSLCIGCKACEVACKV
                     WNDVPEDGLMLTGMSYDNTGALGADTWRHVAFVEQTRPATTTDLGMPGLGPPAADPQG
                     AVEVGSEETDGIAQLRWLMSSDVCKHCTHAACLDVCPTGALFRTEFGTVVVQPDVCNG
                     CGYCVPACPYGVIDQRKDDGRVFKCTLCYDRLKVGEEPACAKACPTDSIQFGELSALR
                     QRAESRVAELHEKGVDVARLYGQDPEDGVGGDGAFFLLLDEPEVYGLPPDPVVTTRDL
                     PDIWRQVTAAAVLVAGMAVSSFFGRRRR"
     gene            11010..11942
                     /gene="nrfD"
                     /locus_tag="VH419_12930"
     CDS             11010..11942
                     /gene="nrfD"
                     /locus_tag="VH419_12930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009080026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HEY7044566.1"
                     /translation="MNDLNTGRAPEYRSYYGQPILKAPVWKQPDVPLYLFLGGVAGAS
                     SLLAEGAALTGRPHLERVARTLAAGGAGVGTIALVHDLGRPERFLNMLRVFKPTSPLS
                     VGSWLLAPFASLASAALASQLTGRLPRLGRLAGGGAAVFGPPLVTYTAALISDTAVPA
                     WHEAYRELPFLFAGSGSAAAGGLSMLATPVAEAGPARRLAAVGAVVDLTAAELMRRRL
                     GMLAEPYESGRPGMLLRSARLLTALGGVGALAGGRSRAVSGASGLALAAGSVLTRFGV
                     FQAGLASAKDPKYVVVPQRERLEQRRDEDRTLAG"
     gene            11939..12958
                     /gene="selD"
                     /locus_tag="VH419_12935"
     CDS             11939..12958
                     /gene="selD"
                     /locus_tag="VH419_12935"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013861436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HEY7044567.1"
                     /translation="MTVQDNVGGATAVGVRLTQYAAGGGCACKVPPGELERVVAALPR
                     AGTTAYELLVGLDSGDDAAVVRISDHEAVVTTADFFTPVVDDAYDWGRIAAANALSDV
                     YAMGGTPLVAVNLLSWPRDRIPFELAGEVMRGGADVARQAGCHVAGGHSIDDPEPKYG
                     LAVTGIADPRRLLRNDAATPGLPLTLTKPLGLGVLNNRHKATGERFDEAVAVMTQLNA
                     DAARAALARGLTAATDVTGFGLLGHLYKLVRASGVSALIDSRAVPFLAGAREAAAAGY
                     VPGGSRRNLDWVRPHLDASVDADELILLADAQTSGGLLVVGEIPGYPVIGEVLPPGPV
                     PITVR"
     gene            complement(13034..13720)
                     /locus_tag="VH419_12940"
     CDS             complement(13034..13720)
                     /locus_tag="VH419_12940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044568.1"
                     /translation="MRRMMAGIVVGATALLVSSTGQPPADAVNLPESNAGAALANLPA
                     PMLGVNCPLWCPEDPAEPLTISAPPAAKTKASDKGSVQIARNATRKYHSLARAKKHGY
                     RLLKDKNGVACIAMPGMGGMGVHFANGDLVGTPSVHLRRPEALVYDRENGRRRLVALE
                     YVVLKKDWESVHGDNAPRPRLYGHRFNLTRAGNRFGLPAFYSLHAWIWKHNPDGRFAM
                     WNPNVHCKCC"
     gene            complement(13717..13968)
                     /locus_tag="VH419_12945"
     CDS             complement(13717..13968)
                     /locus_tag="VH419_12945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017933781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HEY7044569.1"
                     /translation="MPLYMDIHTLPDKVTFDDVAKAHMADLQVQSRFDVRYLRYWVDE
                     QAGKIFCLVEAPSSDASTTVHREAHGLVAEEVYEVKEGS"
     gene            complement(14069..>15247)
                     /locus_tag="VH419_12950"
     CDS             complement(14069..>15247)
                     /locus_tag="VH419_12950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011751625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY7044570.1"
                     /translation="EYAETIPLARRAREVAEPLGLVDVVSDALNTEACAAAKRGEVWT
                     GLMHRALDIAKENGRHEQAGRAYANLYAEYDLELRIAEGEGIYVEGLAYCDEHDISTF
                     STCLRGERTAVLERLGRWNEAATLATELLRALGQSSVNRLNPLISLGKALARQGSDGV
                     WECLDEAGALADTLGEPSWTTLTHLARAEARWLEGRDDLAVAELDLIPDSDGVTAFDI
                     AWTAVWRRRITGDSAPIECRGRVGEPFVSELGGDVVRAARLWEDRGLRYDAALALLGS
                     ADEAALRDALARLEALGARPAVALARRIMRERGVKSIPSGVRASTRTHPAGLTRREQE
                     VLELVCDGLTNEEISSRLFLSVKTVDHHVSAILGKLGVPSRKLAAAEAAKRGLLSAAT
                     "
BASE COUNT         2357 a         5216 c         5418 g         2256 t
ORIGIN      
        1 ccccccgacc ccgccggtgg agggcacagc ggcagcaccc gccccggcgg gtggccgcga
       61 cgttgacacc cgcatcgcac gctcgtgagc ggtggtcacg acgccgtcga gcagcatcac
      121 ggtggtcacg aggaagcgaa acaggcgccc agcagccaac gggccggcgc gacggccgcg
      181 cttcgccagc cgccgtggag agcgccacgg gtgaccccgc caggtcgtcg tcacgggttt
      241 cctcctgatc gactcgtctg acagcgatga cgctaggcgg cgcagccggc gccgcgacca
      301 acgctgatcg agcgtcaggt gaacaccgat ggacacaagg gaagcctgtt gcgccgaggt
      361 ggcagtgagc gaccgacacg ccgtcaaact ccaacgaaag ctgccacctc ggcgtgagcc
      421 gggaggtcag gcggctgcgt cagggcgcgg ggaactcgaa ggcgacgcgc cggccgccta
      481 cggtgccgcg ggcgatggca cgggcatgtc ggtcgaagtc gttccaccgc aaccggagat
      541 gggctcgaag cgcgccggtc ccggtcgtcc gcaccgaagc gacgacgagg ccggagtgct
      601 gacgccaccg catccggccc gacacggcga cgtcacgcac ccacttgacg ccgtccaggt
      661 cccacgtgac gaccgggtgg ggagcggtgt aggagccgcc acgagtggtg aaggtcccgc
      721 cctgcagccc cacgccgttg tagccgtaca tcgacagcca gcgcgccatg acgtcggcgg
      781 ccgtcgcggc agcgacccgt gcagtgcggt accgctcgcc gtgccagccg gtgcctgcgg
      841 cggtacggac gaaccggtcg acgagccggt tctcgtggta ccggcgggcg cacgacgtgt
      901 cgccggggtc gcggtgccgg acgaaccgtc ggacgatcag cgacgcgcac acgtcgaagt
      961 cacccagcgc ggagacgtga ccggtgttcg cggtctcgac gaacgtcgag ttcgggaagg
     1021 cgttggcggt gtccctggca ccctcgggcg aggtcagcga gtcgaggtcg ccgccgagga
     1081 cgagcgtcgg aacgtcggga tacgcagcat gcggcggcac cggatggacc catcgactcg
     1141 gagccggcca gcgcaggcag tcgtcgtagt acccgtaggg cgacgacacc cactcgcgga
     1201 cgcggaacgg cgcgaagacg cctggccggt cgcgctgcag gcgggtgatg ctcctggcga
     1261 actggcggta ccgggtcttc ggcggcgccg tgacgtcgta cggctgcgga tagtcgttgc
     1321 acgccaccgc gacgtacagc ccctccgagt actcgtcgac tggacctgcc ccgccgacgt
     1381 acaccgtctc gcgcgccagc cgcagcagcg gttgggtgta ggggtgcttg cggagaacgg
     1441 cccgggcggc cgcgtcgagc tcgcggtaga gcgtcggcgt cgtcgcggct cctgtcacca
     1501 tgtcgatgag gttgttgacc gtcaccctga tgtgcctggt cacaccgtcg gcgttgggag
     1561 cgtcaccgct gatcgggtga cggcggagca gctgggcgag tctggcgagc cggtgcatcg
     1621 gcgtaccggg gtggtgagcg caagccgggc tccggtcgca ggccacggtg aaggcgtgcc
     1681 ggagggcgcg gttggtgtcg gagtaccacg ggtccttccc accgacgaag taggccgcgt
     1741 cgagcgtcag cgtgcggagc cggtcgccgt ggcggagcgc gaacgtctgg gagaagaacg
     1801 tgccgtacga gtcgccgtac aggtcgacct tcctgatcgc gaacctgtcg aggaccgcgg
     1861 ccaggtcgtc ggtggcgaat gcagtcccgt acacgtcgga cgtcgcgccg agccgatggc
     1921 cgcacctccc gatcgcaagg ttccggttgc cccggtagga ctgcagccac gggcaccgga
     1981 ccgggctcga cttccccgtc ccgcggttgt ccacgagaag cagccggcgc cggtcgagca
     2041 ggggcccgaa cagatcgagg tagtagctgc ggctgtcggt cgtcgagtac cccggcccac
     2101 cttcgaccgc cacgatcgtc cccagccccg gccgggaccg gtcgcggcgg tggtagagct
     2161 cgtacgcgat cccgatgtgg tcgtaggcgg gatcgttcgg gtcgagcaac cgccgaacgt
     2221 ggccgcatcg tgcttcgccg tggtgacagg atcggtcgct gaccgtgcgg tcggccttcg
     2281 gttcgccggc aggaacgtcc ggaatcggta cggcgaccgg cgccgttcga tgctgggcgg
     2341 ccgcgccgtc cggagcggac agcgacaccg tcaggagcga gacgagtaca gcgacgatcc
     2401 acctgggttg cctgatcatg gtcacctcga acgtcgatcc ccgcgagcgc gagtctgact
     2461 cgcgttgaac cgtcggtcaa gcccctggga cgcaccgcga atagcgggaa acgtcacccg
     2521 ctgtcggggt ctcggcccgc acctttcccg caattcgcgg aagccacggt cggccggtca
     2581 gtgggttagc ggttgcgcag cgcggggagg acctcgtcgg cgtacatgct gagcatgtcg
     2641 gcgaagtgcg gtcccatgtt cgcgacatag atctcgtcga acccggtgtc gaggtatggc
     2701 gagaacgcct tgacgtgctc actggcatcg ttgccgcaca caaccgagga gcgggtcatc
     2761 tcttcggtga cgaggctgga cgcctgctcg aaatgccgcg gcgacggcag cacttgcgcc
     2821 agctcgcccg gtaggccgct gttggcccag agtcgatgcg cgatcttcgc gccttcgtca
     2881 ggggtctcgg cgtagcacac cttgaaaccg ccctggaccg gcttgtccgc cccggcctgg
     2941 tcgcggaacc gcttgacgag cgtgtcgtcg ggtgacgtcg tcacgtaccc gtcgccgatc
     3001 cggccggcga cgtcgaccgc cttcggcccg aagcctgaca cgtagaccgg aggcggctcc
     3061 tcggggagcg tgtagatgcg tgcggtgtcg acggtgtagt gccggccccg atgtgtcacg
     3121 acgtctccgg accagagctc gcgcatcacc tcgacggcct cctcgagcat ctccagccgc
     3181 acgtcagcgg tcggccacgc ggtcccgagc acgtgctcgt tcaacgcctc tccggagccg
     3241 acgccgagac ggaaccgacc gtcgagcagc acggcacttg tcgccgcagc ctgcgcgatg
     3301 accgcgggat gggtccgtat cgtcgggcag gtgacggcgg tggtgaccgg caggctgcac
     3361 gcctgtgaca gcgccccgat catggaccac acgaacgggc tctcaccttg ctcgtcgttc
     3421 catgggtggt agtggtcact gatccagagc ccgtcgaagc ctgcctgctc cgcgcgggtg
     3481 gcctgttcga tcagctctgc gggcgagtac tcctcgcacg acaggaagta tccgaaccgg
     3541 gtcatgcgcc cggtgtaccc gaacgcgttc gccgcatgcc cggcagcggg cggaaggtca
     3601 gatgacggtg cggcggtcgt ccgcgaggcg gcgcgtcaga ccggcgcggt cgaggtgcgc
     3661 gaggagcgga aggaccacgc gcctggacgt gtcgagcttc tgcctggccg cactcgtcgt
     3721 gaacggttga ggcagggttc gcaggcactc gacggcctgc tggtcggcgc cgggcagcag
     3781 gacgacggtc tcaccgacac gcagcacccg cccggcgcgc tcggccgccg ccagcgaacg
     3841 acggtcgagg cccagctcct tcagtcggtc ggcatcgggc gcctggaacg ggtgcaaccg
     3901 gaactggtcc tcgagctgtg acagcgccgc ctccagcgcg cccggcaagg ccgtggactg
     3961 acgacggatc cgcccgtcga catacgccag tggaggccgc accaccgtca gggcgagccc
     4021 cgggtccggc agctcgagca gccgagcggc agcggccggg gtcaggccgt cctcgagtgg
     4081 gtcgtgtgca gtctgcgcgg cgactgccgc gaccagccgt tgcgacagct cggacagtcg
     4141 ggcgggatcg agcagccacc cgctcgccgc agagcgaccg tccgtggccg acgacagctg
     4201 atcccgagga acgcccaggc ggcggagcag gccggtcgcc gccatgcctc gccggcgcag
     4261 ttcctcatcg aggtcaggtg tcccgctcgc agcggccagc gcatcggcac gtcgccggcc
     4321 gtcgccgcgt cgctccaggc cgggcggtgc gggatcgagg acgcggcatc gccacaccag
     4381 ccgccgacca gggtcgcgca gcagggcgac gtcacccacg tgccacggca gcggccgggc
     4441 caccctgacc cggacgagcc agccgtcccg cgtcgtggcg aggggccgga ccctggcgct
     4501 caccgacgcc gagcccacgt gcagcaccgg ctccgccggt ggtgtccccg gtccggtcac
     4561 gctgacatcg aggaggtctg aggtgtacca agccccgggt gtcgtcaggg ccgtgcctcg
     4621 gcgcaacccc gtcggcacgc cggatccgag ccggagcgag acgcgtgcga cgccggtggc
     4681 ctggtcgatg tctttggtca tcgactgcag tccgcggaca tggatgaggt cggagtcgca
     4741 cgccagcgtg tcgcccgttc ggatccgccc agccggcagg gtgccggtca cgacggttcc
     4801 cgcaccgggg cgggtgaaca tccggtcgac ccagagtcga acgtcggccg cgggatctgg
     4861 tggtggtgca gacgccagca cccggtcgag cgtcgtacgc agctcggaca gcccctcgcc
     4921 ggtgacgccc gacacgatca ccgcagggat gtccgccagc gtcgtggcag ccgtcgcagc
     4981 gcgggccttt cgtagtgccg cctccgcatc accgaggtcg gacctggtga cggcgaggac
     5041 acccgtgttc acctcgagcg cgtccaggat cgccaggtgc tcgtccgcct gcggcatcca
     5101 cgggtcgtcg gcggccacca cgaacaacac caccggcacc ggcccgaccc cggcgagcat
     5161 cgttgagatg aagcgttcat gacccggtac gtcgacgaag gcgacgtcgc cggagcgggg
     5221 gagaccggtc cagcagtagc cgagctcgat ggagaggccg cgccgacgtt cctcggccag
     5281 ccggtccggc tgctgtccgg tcaaggcctc gatcaacgtc gacttgccgt ggtcgacgtg
     5341 tccggcggtg gcgacgacgt gcaccctcag tcgacctcgg tgctctcctt ggctgcccgg
     5401 cgtacctgct cgaccaggat cgtgtcgtcg cgaggatcga cggcgatgag gtcgagaaga
     5461 agccggtcgt ggtgcacccg acccagcacc gccgggtcgc cgagccgcag cggcaccgcc
     5521 agcccggtcg gcagcgcgat cgccgcactg gggagatcga ccccaggcgc cccgccaccc
     5581 ccgatcgccg ccagggtctc gacgacctct gcgtggacgt gtggctccag cttcccagcg
     5641 agcgccgtgg cgcgatctcg cagcgctgcc tgctcggtga ccagggcacg gctgaccggg
     5701 cccggtggac cggacagcgt ggcctcgagt gccgccagcg tgagcttgtc gacgcggtac
     5761 gcccgggcaa gcggatggcg gcgcacccgc ccgacgaggt cagcacgccc catcagcagc
     5821 ccgcactgtg gtccgccgag cagcttgtcg ccggacgcgg tgaccagggc agcgcctgct
     5881 tcgagcatgg tcgttgcatc cggttcgtcc ggcagccgcg gatggggatg cagcagtccg
     5941 gacccgatgt cggcgaccac cggcacgggc agcgcgctga ggctgcggac cgggacggac
     6001 gacgtgaagc cggagacgac gaagttcgac ggatggacct tgaggacgaa ggcggtgtcg
     6061 tcaccgatgg cgtcggcgta gtcaccgagg ccgacccggt tcgtcgtgcc gacctcgcga
     6121 agccgcgcgc cggttgactc gagcagctcg gggatacgga acccgtcgcc gatctccacg
     6181 agctctccgc gcgcgacgac gatcgaccga ccgtgagcca gcgccgacgc caccagggcg
     6241 agtgcggcag cgccgttgtt gaccacgtgc acgtcctcgg ccgcggggac gctggtggcc
     6301 agtgcggcca gcgtggagcg accgcgtcga ccgcgccggc ccgacgccag gtcgagctcc
     6361 acgtctgtgg atcgcgcagc gacttcgagg gcgctgactg cagcgtcgga gagtgcggcc
     6421 cggccgaggt tggtgtgcac gagcacaccg gtggcgttga cgacgcgtcg caggctggtg
     6481 ggtgccgacg gcagcagtcg taccgcctgg tcccggaccg ccgtggccgg gagtcgcccg
     6541 tcccgggctg ccgcctgtgc ccgccggatc gcgtcgagga cggtgcgcct gccgagccgg
     6601 tcgcatgcct cggcgaggac cgggtcggcg agcagcatgt ccgtggccgg gatggcccgg
     6661 cgtggatcgt ccatgttcga cctcggttcg agctcacggt cggcagctgg cggaggcgga
     6721 cgggaatcga acccgcctga ccgagatgct cggtcacgtc ggttttgaag accgcgctcg
     6781 tcaccaggcg agatacgcct ccgccgccac cctatgctga gttgcgttcg gtcgagatag
     6841 agagagactc gactcgagca ccccgggaag gactgcgatg acgacgaccg agcagacgtt
     6901 cctcccgtgg cccgcggttc gccagctcct cggacctgac cggctggggc gcggcacggc
     6961 ggcacagtca gcgcgcagcc gaacgctccg gccgcggatc gcggacgccg atcgcgtcgt
     7021 cgactcgatc tgtccgtact gcgccgtcgg ctgcggccaa caggtctacg tcaaggacga
     7081 gcgcgtcatc cagatcgagg gtgacccgaa cagcccgatc tcgcgcggtc ggttgtgtcc
     7141 caagggcagc gccagctacc agctcgtgac atctccccag cgggtcacga aggtgcgcta
     7201 ccggcgcccg cgcggcaccg agtgggagga cctcgacctc gacgtggcga tggacatgat
     7261 cgccgaccgg gtgatcgcgt cgcgtcgtga gtactggcag gagtactccg acgacggtgt
     7321 ccggctgcgg cgtaccttgg gcatctcgag cctcggagga gcgaccctcg acaacgagga
     7381 gaactacctc atgaagaagc tctacaccgc gatgggcgcc atccagatcg agaaccaggc
     7441 gcgcatatga cactcgtcga cagtccccgg tctgggggct tcgttcggac gtggcggggc
     7501 cacgaccttc cagcaggacc tcgcgaacgc tgactgcatc ctgatccagg gatccaacat
     7561 ggccgagaac cacccggtcg ggttccagtg ggtcatggag gccaaggctc gcggcgcgac
     7621 gatcatccac gtcgacccgc ggttcacccg cacgtcggca ctggctgacg tccatgccac
     7681 cttgcgggcc ggcaccgaca tcgtgttcct gggtgccctc gtcaaccacg tgctggtcaa
     7741 cgaggcggac ttccgcgagt acgtcgtggc ctacacgaac gccgccgcca tcctcagcga
     7801 cgactacgag gatcccgagg acctgtcggg cgtcttctcc ggcttcgacg aggaccaggg
     7861 ccgctacgac ccgaccacgt gggagtacga acgcgcgcga cggaaggaag aggcaggcgg
     7921 cgagggttcg gaggaggcgc acgcggcaca cgaggcgagc cgctccgagg cggccgggtc
     7981 cggcgggcca acggtcggtg cgaaggccga ccgggacgag acgctgcagc acccacggtg
     8041 cgtctaccag gtcctccggc ggcacttcgc ccgttacacg ccggacatgg tggaggacgt
     8101 ctgtggtgtg ccacgcgagc agttcgtgcg catcgcggaa gccctggaac gcaacagcgg
     8161 gcgcgaccgc acgtcggcga tctgctacgc ggtcgggtgg acgcaacaca gcacgggagt
     8221 ccagatgatc cgcaccgccg cgatcctgca gctgttgctc ggcaacatcg gccgtcccgg
     8281 cggcggcatc atggcgctgc gcgggcatgc cagcatccag ggctccaccg acatcccgac
     8341 gctctacaac atccttcccg gatacctccc gatgccgcac gccgaccagc accagtcgat
     8401 ggaggactac gtgcatgtcg acgaggaggc cgccggattc tgggggaaca tccgcaccta
     8461 cctcgtcagc atcctcaagg cctacttcgg cgacgcggcg acgaccgaca acgagttctg
     8521 cttcgactac ctgccccgga tcaccggtga ccacggcacg taccggatcg tcaaggacca
     8581 ggtcgagcgc ggcggcggcg gctacttcct cgtgggggag aatccggccg tcggctcgtc
     8641 gaacggcaag atgcagcggc tcggactggc caacctcgac tggctcgtgg tccgcgacat
     8701 ggtgatggtg gagtcggcga ccttctggaa ggacggtccc gagatcgaga ccggggagct
     8761 gcgaccggaa gacatcggta cggagatctt cttcctgccg gctgcggcac acaccgagaa
     8821 ggacggcacg ttcacgaaca cgcagcggct gctccagtgg caccacaagg ccgtcgagcc
     8881 gccggacgac gccaccagcg atctgtggtt ctactaccac ctcggcaagc gcctcaaggc
     8941 aaagcttgcg gactcgaccg atccggtcga cgcaccgttg caggacctcg tctgggacta
     9001 cccggaggag ggaccgagag ccgagcccag tgcggacgcc gtgttgcgcg agatcaacgg
     9061 gaccggtccg gacggtgcgg cgctgtcggc gtacaccgag ctgaaagccg acggctcgac
     9121 gtcgtgtggg tgttggatct attgcggtgt gtacgccgac gaggtgaacc agtccgcgcg
     9181 gcgcaagcca ggtagcgagc agagctgggt cgcacccgag tggggctggg cgtggcccgc
     9241 caaccggcgg gtgctgtaca accgggcgtc ggcggacccg gacggcgatc cctggagcga
     9301 gcggaaggcg tacgtctggt gggactccga cgccggcaag tggaccggtc acgacaaccc
     9361 ggacttcatt ccggaccgag aaccacatca cgtgcccgag gaaggtgcga ccggcccgga
     9421 cgcgatcggt ggctcggacc cgttcatcat gcagaccgac gggaagggct ggctgttcgc
     9481 gccgaaaggc ctcgccgacg gcccgttgcc cacgcactac gagccggtcg agtcgccgat
     9541 ccgtaacccg gcgtaccggc agcagtccaa cccggtggcg gtggcgtacg agaccgccgt
     9601 caacccggtc catcccgaag gcgccgaggt cttcccatac gtcttcacga cgtaccggct
     9661 caccgagcac cacacggcgg gcggcatgag ccggacgctt ccgtacctcg ccgagctgca
     9721 gccggagttc ttctgtgagg tgtctccggc gctggcggcg gagcgcggac tgacgcatgc
     9781 cgggtgggcg acgatcgtga ccgcacggac ggcggtggag gcgcgcgtgc tcgtcaccga
     9841 ccggatgcga cggctgcgca tcggtgacca gtgggtccac cagatcgggc tgccgtacca
     9901 ctggggaggc aacggcctga cgcgtggtga ctcagccaac gacctggtca acgtgaccgt
     9961 cgacccgaac gtccggatcc aggacaaggt cggcacgtgc gacatccggc cgggacgtcg
    10021 accgcgtggt gcggcgctgc tgtcgttcgt ggcggactac cgccgccgcg caggcatgac
    10081 cgaggacggg gagcagcgat gagctggtcg gggccgcttc gtgacgtcgc cggggacgcg
    10141 ggccacgtcg accacccacc tcggatgggt ttcttcaccg acacgtcgct gtgcatcggc
    10201 tgcaaggcgt gcgaggtcgc ctgcaaggtc tggaacgacg tccctgagga cggtctcatg
    10261 ttgaccggga tgtcgtacga caacaccgga gcgctcggtg ccgacacctg gcggcacgtc
    10321 gcgttcgtcg agcagacaag gccggcaaca acgaccgacc tcgggatgcc gggactcggt
    10381 ccacctgctg ccgacccgca gggggctgtc gaggtcggga gcgaagagac cgacggcatt
    10441 gcccagctcc gttggctcat gtcgtccgac gtgtgcaagc actgcacgca cgcggcctgc
    10501 ctcgacgtgt gcccgaccgg cgcgctgttc cgaaccgagt tcggcaccgt cgtcgtacag
    10561 cccgacgtct gcaacgggtg cggctactgc gtcccggcgt gcccgtacgg cgtcatcgac
    10621 cagcgcaagg atgacggccg ggtcttcaag tgcacgctgt gctacgaccg tctgaaggtg
    10681 ggtgaggaac cggcctgcgc caaggcctgt ccgaccgaca gcatccagtt cggcgaactc
    10741 agtgcgctgc ggcagcgggc ggagagccga gtggcggagc tgcacgagaa gggcgtcgac
    10801 gtcgcccggc tgtacggcca ggatccggag gacggtgtcg gcggcgacgg agcgttcttc
    10861 ctcctcctcg acgagccgga ggtctacggg ctcccgccgg acccggtcgt cacgacgcgg
    10921 gacctccccg acatctggcg acaggtgacc gcggcagcgg tgttggtcgc cgggatggcg
    10981 gtcagctcgt tcttcgggag gcggcggcga tgaacgacct gaacaccggg cgggcaccgg
    11041 agtaccgctc ctactacggt cagccgatcc tgaaggcgcc ggtgtggaag cagcccgacg
    11101 taccgctgta tctgttcctc ggtggcgtgg caggggcgtc gtcgctgctg gccgaaggtg
    11161 ctgcgctgac cggtcggccc cacctggaac gggttgcccg gacgttggct gccggtggtg
    11221 ccggcgtcgg cacgatcgcg ctcgtccacg acctcggccg gcccgagcgg ttcctcaaca
    11281 tgctgcgcgt cttcaagccg acgtctccgc tgtcggtggg ctcgtggctg ttggcgccgt
    11341 tcgccagcct tgcctcggca gcgctggcat cgcagctgac cggacgtctg ccgaggctcg
    11401 gccggctggc cggcggcgga gccgccgtgt tcgggccgcc gctcgtcacc tacacagccg
    11461 cgctcatcag cgacacggct gtcccggcct ggcacgaggc ctatcgcgag ctgcccttcc
    11521 tcttcgccgg cagcggcagc gctgcagcgg gaggactgtc gatgctcgcc acgccggtgg
    11581 cggaggccgg accggcgcgg aggttggcgg ctgtcggcgc ggtcgtcgac ctcaccgcag
    11641 cggagctgat gcgacggcga ctcggcatgc tcgcggagcc gtacgagagc ggccgacccg
    11701 gcatgctgct ccgctcggcc cgactgctca ccgctcttgg cggggtgggt gcgctcgcgg
    11761 gtgggcggtc gcgcgctgtc tcgggggcgt ccggcctggc gttggccgcc gggtcggtcc
    11821 tcacgaggtt cggcgtcttc caggccgggt tggcatccgc caaggacccg aagtacgtcg
    11881 tcgtgccgca gcgcgaacgg ctcgagcaac gccgtgacga ggaccgtacg ctggcgggat
    11941 gacggtccag gacaacgtcg gcggtgcaac tgctgtcggt gtccggctga cccagtacgc
    12001 cgccggtggt ggatgtgcct gcaaggttcc gcccggcgag ctcgaacgtg tcgtcgccgc
    12061 gctcccgagg gccgggacga cggcgtacga actgctggtg gggctcgatt cgggagacga
    12121 cgcagcggtc gtccgcatca gcgatcacga ggcagtcgtg acgacggcgg acttcttcac
    12181 acccgtcgtc gacgacgcgt acgactgggg ccggatcgca gcggccaacg cgctgtccga
    12241 cgtctacgcg atgggcggta caccgctggt ggcggtcaac ctgttgagct ggccacggga
    12301 ccgtatcccg ttcgagctgg ccggcgaagt catgcgtggc ggcgccgatg tcgcgcggca
    12361 ggccggctgt cacgtcgcag gcggtcacag catcgacgac ccggagccga agtacggcct
    12421 ggccgtgacc ggcattgccg atccgcggcg gctgctgcgc aacgacgcgg caacgccagg
    12481 ccttccgttg acgctgacca agccgctcgg gctcggcgtg ctcaacaacc ggcacaaggc
    12541 caccggtgag cgcttcgacg aggcggtggc ggtgatgacg cagctgaacg ccgacgcagc
    12601 acgggcggcg ctcgcgcgcg ggctgacggc cgcaaccgac gtgaccgggt tcggtctgct
    12661 cggccacctg tacaagctcg tccgcgccag cggtgtctcg gcactgatcg actcgcgcgc
    12721 ggttcccttt ctcgccgggg cccgcgaggc cgctgcagcg ggttatgtcc caggcgggag
    12781 ccggcggaac ctggactggg tccgcccgca cctggacgcg agcgtggacg ccgacgagct
    12841 gatcctgctc gccgacgccc agacgtccgg cggcctgctg gtcgtcggcg agatcccagg
    12901 ctatcccgtc attggcgagg tgctgccgcc cggcccagtt cccatcactg tccgctagcg
    12961 gcacgcaagt gcggggagtt ccgggatctt gcggcgcaag atcgcggaac tccccgcagt
    13021 tcgcgggagg cggtcagcag cacttgcagt ggacgttcgg gttccacatc gcgaacctgc
    13081 cgtcggggtt gtgcttccag atccatgcgt gcagcgaata gaacgccggc agtccgaacc
    13141 ggttcccggc acgggtcagg ttgaagcggt gtccgtacag ccggggacga ggcgcgttgt
    13201 ccccgtgcac gctctcccag tccttcttca gcacgacgta ctcgagcgcg accaggcggc
    13261 ggcgtccgtt ctcgcggtcg tacacgagag cctcgggcct gcggaggtgc acgctcggag
    13321 taccgaccag gtcaccgttc gcgaagtgca cgcccatccc acccatgccc ggcatcgcga
    13381 tgcaagcgac gccgttcttg tccttgagca gccggtagcc gtgcttcttg gcgcgggcca
    13441 gggagtggta cttcctcgtc gcgttgcgag cgatctgcac gctgcccttg tccgacgcct
    13501 tggtcttcgc tgcaggcggc gccgagatcg tgagcggctc ggcagggtcc tcgggacacc
    13561 acagtgggca gttcacaccc agcatcggag ctggcaggtt ggccagggcg gcaccggcgt
    13621 tcgattcggg caggttgacg gcatcagcgg gaggctgccc ggtcgacgac accagcaggg
    13681 ccgtcgcgcc gacgacaatg cccgccatca tgcgtctcat gagccctcct tgacttcgta
    13741 gacctcctcg gccaccagac cgtgggcctc gcggtggact gtggtcgagg cgtcggagga
    13801 gggcgcctcc accagacaga agatcttccc cgcctgctcg tcgacccagt agcggaggta
    13861 gcggacgtca aagcggctct gaacctgaag gtcggccatg tgcgccttgg ccacgtcgtc
    13921 gaacgtcacc ttgtcgggca gggtgtggat gtccatgtag agcggcatcg cagtggtcct
    13981 ttcgtttgca tctgcggata ccgcgaacgt atgtttcgcc gctcggccga gaatcgggag
    14041 cgactaccca ggttgcgccg ttggctacct atgttgcagc gctcagcagc cctcgcttgg
    14101 ctgcttcggc cgcagcaagc ttccgcgacg ggacccccag cttgccgagg atggcggaga
    14161 cgtgatggtc gaccgtcttg accgaaagga agagcctcga cgagatctct tcgttggtga
    14221 gtccgtcaca caccagctcc agcacttcct gctcgcgccg ggtcaggccc gctggatgtg
    14281 tgcgcgtgct cgcgcgtacg ccgctgggga tcgacttgac cccgcgctcg cgcatgatcc
    14341 ttcgcgccaa cgcgaccgct ggtctcgcgc cgagggcttc gagacgcgcc agcgcgtcgc
    14401 gcagggctgc ctcgtcagcg gagccgagca gcgccagcgc cgcgtcgtag cgcagccccc
    14461 ggtcctccca cagccgtgcc gcacgcacga cgtcaccacc gagctcggag acgaagggct
    14521 cacccacccg gcctcgacac tcgatcgggg cggagtctcc cgtgatgcgg cgccgccaca
    14581 ccgctgtcca cgcgatgtca aaggcagtga ccccgtcgga gtcggggatg aggtcgagct
    14641 cggccaccgc caagtcgtca cgaccctcga gccaacgggc ctccgctcgg gcgagatggg
    14701 tcagcgtggt ccagctcggt tcaccgagcg tgtcggccag cgcaccggcc tcgtcgaggc
    14761 attcccagac accgtcggag ccctgtctcg ccagggcctt gccgaggctg atcagcgggt
    14821 tgagccggtt gaccgacgac tgccccagcg cacgcagcag ctcggtggcg agcgtcgcgg
    14881 cctcgttcca acggccgagg cgctccagga cggccgtgcg ttcaccgcgc agacacgtcg
    14941 agaacgtgct gatgtcgtgc tcgtcgcagt acgccagccc ttcgacgtag atgccctcgc
    15001 cctcggcgat ccgcagctcc aggtcgtact cggcatagag gttggcgtat gcccggccgg
    15061 cctgctcgtg gcggccgttc tccttggcga tgtccagggc tcggtgcatg agccccgtcc
    15121 agacctcgcc gcgcttggct gcagcgcagg cctcggtgtt gagcgcatcg ctcaccacgt
    15181 cgaccagccc cagcggctcg gcgacctccc tggcacggcg tgccagcggg atcgtctcgg
    15241 cgtactc
//
LOCUS       DATBFZ010000428         6034 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_957781, whole genome shotgun sequence.
ACCESSION   DATBFZ010000428 DATBFZ010000000
VERSION     DATBFZ010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 6034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6034
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_957781"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2743)
                     /gene="eccCa"
                     /locus_tag="VH419_12955"
     CDS             complement(<1..2743)
                     /gene="eccCa"
                     /locus_tag="VH419_12955"
                     /inference="COORDINATES: protein motif:HMM:TIGR03924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EccCa"
                     /protein_id="HEY7044571.1"
                     /translation="MTTTLVKRPARIAPPGVPTEHITIADPPTQEQTPPAAAGATMIL
                     MPIMSGVGSLTVSLTNRGNPLVAVGGLIFLVGSITVGAIMIISQRSGPKRLLREGRER
                     YLDYVEDLRHSLRETLSAQKRNAAWRHPSPELLPDIARSNARRWERRRTDSDHLVLRL
                     GVGDQPIVAALTMSADTGPLNQFDPVCLDAAVNLQTRYSVVHEQPVTVDLRPLSVLSV
                     LGDRYVSRQLATAMALQLVTFQSPADVSLAIVRSDDLAEAWEWAKWLPHCQSTTTMDG
                     DVPARLVTTSVGGMAELLADEFERRLTERARTRGREPDPRDRLVVIVDGEGLTTVWGL
                     DSPDPSVSLAELGIHVVMLLADRREEPEAVDDRIEVRPDFVAVQASRPAPFAVDPVAE
                     GLPVLVSRQLAPLRVSVEDRSGGGSLEATVGLPEILGVDDPATLDARVTWRKRSLRDL
                     LRVPIGVGANGGVVLLDLKESAHGGMGPHGMVVGATGSGKSEMLRTLVSSLVISHGPE
                     RLALMLVDFKGGATFAAMEDIPHLAGMITNLQDDLTLVDRMRDALFGEMQRRQEILKQ
                     AGNLPNVTAYQDLIDAGEDLEPLPHLLVIIDEFSELLTAKPDFAELFVAIGRIGRSIG
                     VHLLLATQKLEMGKIRGLESHLSYRISLRTFSESESRDAIGVPDAYHLPPEPGSGYLK
                     VDTTVFERFKAALVSSPYTPPSEGPKTEVPAVPYLAVNGLGAWIADMNAAAEAAAKSE
                     ETSGRLSGKNERSVLDVVCEQLLAPGAERVRPVWLDPLPAHLPLDRVLEPADTGEPAS
                     VRAILGLVDEPAKQRQFPLEWDFAGAAGNVMVVGAPQSGKSVLLRTMISSLCLRHAPS
                     DMVFYCLDFGGGTLRALTDVPHVATVTPRTDVERVTRTVNDVLTQLEGRDALFREHGV
                     "
     gene            2884..4278
                     /gene="eccD"
                     /locus_tag="VH419_12960"
     CDS             2884..4278
                     /gene="eccD"
                     /locus_tag="VH419_12960"
                     /inference="COORDINATES: protein motif:HMM:TIGR03920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion integral membrane protein
                     EccD"
                     /protein_id="HEY7044572.1"
                     /translation="MLTSYSRVTVVSGSRRVDLALPSGLPVADVVPQVLRFCAPEESD
                     GEPTEFTLARVGGQSLSLSQSLAEAGVHDGDVIELRSFSAEARAAFVEDVRDAIEDSV
                     DAAGGAWTSRSTVTFCVVTTCGVLTLLLTPLLLDVWQRVMSGDPAFLKSSEAVTGQRV
                     TSAFVTAVLLLGSSWIGTRWAARWTPYIALTAAALWAFLAGVELASHSTSDTTVALTT
                     GVGASTVVAGLGRLMTARATPLLAANVVLLGSLAVVFVADQVGADLGIVVRVVAALAV
                     LSIGMMPRLSIAVGGLSSADYRVRNAGRVSDRVLSARFQESSGLLLGSVIGVSVAVSL
                     IGFWLGLHGNDTTQHEWDRLLSLSLAATLLLRSRVFSRVQFMLPPRIGSVFILLASAI
                     QLARDYDALETWLYAAIAAVGVLAVVVSIVHLSDVTRARVKRILNLVEFIVVVDLVVV
                     TMGAVGLYDYVRGT"
     gene            4338..5564
                     /locus_tag="VH419_12965"
     CDS             4338..5564
                     /locus_tag="VH419_12965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044573.1"
                     /translation="MDDDLTPEEAAEQEAERRRSSAQGAAGDLARMGIDPRALGLQPP
                     ARTAPRRSNGRQEQPEGHAQQAPAQSPAQPANVVPLRPAGPVLPPSGTSSAWAPSPPP
                     LASGSPADVETRAVPLEFLLGREPGRELPRPSVAQLARAVTFGMLTLDAADAADRERA
                     MVNRVRTRQTDQRTVVFLAGKGGVGTTSVAIGVGCVLAALRDDTTTLVSMRPGTPSLG
                     LALAGTPAPNARELAHADADVSPLLLNNGLQILDGPRWGTPVRRSDVPAIVDRLAQAS
                     TFSLFDVGNDASESGHSILSRADQVVIVSSSGNDGIDGARVAAERAAEIDPYFLDSAI
                     YVVVCRRESALRTVVKAMRETMPQGARIVAVPPEDTLADGEAFDPSRVSVATRLAMID
                     IAGLVALGSVTQTVRR"
     gene            5566..5952
                     /locus_tag="VH419_12970"
     CDS             5566..5952
                     /locus_tag="VH419_12970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044574.1"
                     /translation="MSGPPFHPPSGPSRSTSAGQPDEQRRQPTPWTPPNASSTPNAER
                     YLLEADALRRRQLRSALLHGSSRTWRDQGRIWPGVIAGIVVVTVILASFAVMKAFEKS
                     EELQKEQESPTAPAATSPATPTQSTR"
BASE COUNT          910 a         2126 c         2072 g          926 t
ORIGIN      
        1 acacgccatg ctcgcggaag agcgcgtcgc ggccctcgag ctgggtgagt acgtcgttca
       61 cggtgcgggt cacccgctcg acatccgtgc gcggcgtcac cgtggcgacg tgcggcacgt
      121 cggtcagggc acgcagcgtg ccgccaccga agtcgaggca gtagaaaacc atgtccgacg
      181 gcgcgtgacg aaggcacagc gacgagatca tcgtccgcag cagcacgctc ttgccggact
      241 gcggagcccc gaccaccatc acgtttccgg cagcgccggc gaagtcccac tcgagcggga
      301 actgccgttg cttcgccggc tcgtcgacca gtcccaggat cgcgcggacc gatgccggct
      361 cgccggtgtc ggccggttcc agcacccggt cgagcggcag atgagcgggc agcggatcga
      421 gccagaccgg acggacccgc tccgcccccg gcgcgaggag ctgctcacac acgacgtcga
      481 gcaccgaccg ttcgttcttc cccgacagcc gcccggaggt ctcctcgctc ttcgcggccg
      541 cctcggcggc agcgttcatg tccgcgatcc aggccccgag tccgttcacc gccaggtacg
      601 gaactgccgg gacctcggtc ttcggccctt cgctcggcgg cgtgtacggc gacgacacaa
      661 gcgccgcctt gaaacgctcg aacaccgtcg tgtcgacctt caggtagcca gaacccggct
      721 ccggcggaag gtggtatgcg tccggtacgc cgatcgcgtc ccgggactcc gactctgaga
      781 acgtgcggag cgagatgcgg tacgacaggt gcgactcgag accacggatc tttcccatct
      841 ccagtttctg ggtggcgagc agcaggtgta cgccgatcga ccgcccgatc cggccgatgg
      901 ccacgaacag ctccgcgaag tcgggtttgg cagtcagcag ctcggagaac tcgtcgatga
      961 tcacgagcag atgggggagc ggttcgaggt cctcgccggc gtcgatcagg tcctggtacg
     1021 cggtgacatt gggcaggttc cccgcctgct tcaggatctc ctggcggcgc tgcatctccc
     1081 cgaacaacgc gtcgcgcatc cggtccacca gggtcaggtc gtcctgcagg ttcgtgatca
     1141 tgcccgccag atgaggaatg tcctccatcg ccgcgaacgt cgcgccgccc ttgaagtcga
     1201 ccaacatcag cgccagtcgt tccggcccat ggctgatcac cagtgacgag acaagcgtgc
     1261 gcagcatctc cgacttgccc gacccggttg cgccgacgac catgccgtgt ggccccatgc
     1321 cgccgtgtgc cgactccttc aggtccagca gtacgacgcc gccgttggcg ccgacgccga
     1381 tcgggacgcg cagcagatcg cgcagcgagc gcttccgcca ggtcacccgc gcgtccaacg
     1441 tcgccgggtc gtccacgccg aggatctccg gcagccccac ggttgcttcg agggagccgc
     1501 cgcccgacct gtcctccacc gagactcgga gcggcgcgag ctgacggctg acgagcacgg
     1561 gcaggccctc ggcaacgggg tcgaccgcga acggtgccgg acgcgacgcc tgcacagcga
     1621 cgaagtcggg gcggacctcg atccggtcgt cgacggcctc gggctcctct cggcggtcgg
     1681 ccagcagcat cacgacgtgg atgccgagct cggccagcga cacgctcgga tccggcgagt
     1741 ccagccccca gaccgtggtg agaccctcac catccacgat caccacgagg cggtctcgcg
     1801 gatcgggctc ccggccgcgc gtgcgcgcgc gttcggtcag ccgccgctcg aactcgtccg
     1861 ccagcagctc ggccatgcca ccgacggatg tcgtgacgag ccgcgcgggc acgtcaccgt
     1921 ccatcgtcgt cgtcgactgg cagtggggga gccacttcgc ccactcccac gcctcggcga
     1981 ggtcgtcgga gcggacgatt gcgagcgaga cgtccgcagg tgactggaac gtcacgagct
     2041 gaagcgccat cgccgtcgcg agctggcgtg agacgtagcg gtcgcccagc acgctcagca
     2101 cgctcagcgg acgaaggtcg accgtgaccg gctgttcgtg cacgacggag tagcgcgtct
     2161 gcaggttcac cgcggcgtcg agacagacgg ggtcgaactg gttcagtggt ccggtgtcgg
     2221 cgctcatcgt cagggccgcc acgatcggct ggtcgcccac cccgaggcgc agtaccaggt
     2281 ggtctgaatc ggtacgccgt ctctcccagc gccgggcgtt cgacctggcg atgtccggca
     2341 gcagctcggg cgacgggtgc cgccacgcgg cgttcctctt ctgcgccgac aacgtctcgc
     2401 gcagcgaatg acgcaggtcc tcgacgtagt cgagatagcg ctcacggccc tcccgcagca
     2461 gccgcttggg gccggagcgc tgggagatga tcatgatcgc gccgacggtg atcgagccga
     2521 ccaggaagat cagaccgccc actgcaacca acgggttccc gcggttcgtc aacgagaccg
     2581 tgagcgatcc gaccccactc atgatcggca tcaagatcat cgtcgcgccg gccgcggcag
     2641 gcggagtctg ttcctgcgtc ggcggatcgg cgatggtgat gtgctcggtc ggcacgcccg
     2701 gcggagcgat ccgggcgggt cgcttcacca acgtcgtggt caccggtgcc tccgagcaga
     2761 atggggtggt cgcaacggtt atgctccctc acgattgttg cgagtgttcg agaggcactc
     2821 aacacggggc actcgagaac agagtcgccg tcagccagcg aagcactagc ggggtacagc
     2881 ccgatgctca cgtcgtacag ccgcgtcacg gtcgtcagcg gcagtcgccg ggtcgacctc
     2941 gcgctgccga gcggactccc ggtcgccgac gttgttccac aggtgctgcg gttctgcgcc
     3001 ccggaggagt ctgacggaga gcccacggag ttcacgcttg ctcgtgtcgg cggccagtcg
     3061 ttgtcgctct cgcagagtct cgcagaggcc ggcgtccacg acggcgacgt gatcgagctc
     3121 cgctcattct ccgccgaggc acgggccgcc ttcgtcgagg acgtacgtga cgcgatcgag
     3181 gactcggtcg acgccgccgg cggcgcctgg acgtcgcggt cgaccgtgac gttctgcgtc
     3241 gtcaccacgt gcggtgtcct cacgctgctg ctcacgccgc tcctcctcga cgtgtggcag
     3301 cgggtgatga gtggcgatcc cgcgttcctg aagtcgagcg aggcggtgac cggccagcgg
     3361 gtgacgagcg cgttcgtcac ggcggtgctg ttgctcggct cgagctggat cggcacgcgg
     3421 tgggcggcac gttggacgcc gtacatcgcg ctgacagccg cggccctgtg ggcgttcctg
     3481 gcaggcgtcg agctcgcgtc gcacagcacg agcgacacaa cggtcgcgct cacgaccggt
     3541 gtcggcgcat cgacggtcgt tgccggcctc ggtcggttga tgaccgcgcg ggcgaccccg
     3601 ctgctggcgg caaacgtcgt gctgctcggc tcactggccg tcgtgttcgt ggccgaccag
     3661 gtgggggctg acctcgggat cgtcgtccgt gtggtggcgg cgctggcggt gctgagcatc
     3721 gggatgatgc cccggctgtc gatcgccgtc ggtggtctgt ccagcgcgga ctatcgcgtc
     3781 cgcaacgccg gccgggtgtc cgaccgggtg ctctcggcac gcttccagga gagcagcgga
     3841 ctgctgctgg gctccgtcat cggggtgtcg gtcgcggtct cgctgatcgg cttctggctc
     3901 ggtctgcacg gcaacgacac gacgcagcac gagtgggacc ggctgctcag cctgagtctg
     3961 gccgcgacgt tgctgctgcg ctctcgagtg ttcagccgtg tccagttcat gctcccgccg
     4021 cgcatcggca gcgtgttcat cctgctggcg tcggcgattc agctggcccg ggactacgac
     4081 gcgctggaga cctggctgta cgccgccatc gccgctgtcg gcgtactcgc cgtcgtggtc
     4141 agcatcgtcc acctctccga cgtcacccgc gcccgggtga agcgaatcct caatctggtg
     4201 gagttcatcg tcgtggtgga cctggttgtt gtcacgatgg gggctgtggg gctctacgac
     4261 tatgtgcgcg gcacgtgagc gaccgggccc gggacggcag agaggaatga tggacagcca
     4321 gcgaaggaag gtcgccgatg gacgatgacc tcacgccgga ggaagccgcc gagcaggagg
     4381 ctgaacgccg caggtcgtca gcccagggtg cggcagggga cctggcccga atgggtatcg
     4441 acccgcgcgc cctcggcctg cagccgccgg cccgcacggc gccacgacgg tccaacggtc
     4501 gtcaggaaca gcccgaggga catgcccagc aggctcccgc gcagtcgccc gcccagccgg
     4561 ccaacgtggt accgctgcgt ccggccgggc ctgtcctccc accttccggg acgagctccg
     4621 cgtgggcgcc gagcccaccg ccgctcgcgt ccgggtcacc agccgacgtc gagacacgtg
     4681 ccgtcccgct ggagttcctg ctcggtcgcg agccggggcg cgagctccct cggccgtcgg
     4741 tcgcgcagct cgcgcgggcc gtgacgttcg gcatgctcac gctggatgcc gccgacgcgg
     4801 ccgaccgcga acgcgcgatg gtcaaccgcg tacggacccg acagacagac cagcgcacgg
     4861 tcgtcttcct cgccgggaaa ggcggcgtcg gcaccacgtc ggtcgccatc ggcgtgggct
     4921 gcgtgctcgc tgcgctgcgc gacgacacga cgacgctcgt ctcgatgcgc ccgggcacgc
     4981 cgtcgctcgg actcgccctc gcgggcacgc ccgcgcccaa cgcccgagag ctcgcacacg
     5041 ccgacgccga cgtcagcccg ctcctgctga acaacgggtt gcagatcctc gacggtccac
     5101 ggtgggggac gccggtacgc cgcagcgacg taccggccat cgtcgaccgg ttggcacagg
     5161 cgtcgacgtt ctccctgttc gacgtcggca acgacgcaag cgagtccggc cacagcatcc
     5221 tcagtcgcgc cgaccaggtc gtgatcgtga gcagctcagg caacgacggg atcgacggtg
     5281 cacgggtcgc cgcggagcgc gccgcggaga tcgacccgta cttcctcgac tcggccatct
     5341 acgtcgtcgt gtgccgccgc gagtccgcgt tgcgcacggt ggtcaaggcg atgcgggaga
     5401 cgatgccgca aggcgcccgt atcgtcgctg tgccgccaga ggacacgctc gccgacggcg
     5461 aagccttcga tccgtcgcgg gtcagcgttg ccacccggct cgcgatgatc gacatcgcgg
     5521 gcctggtcgc gctcggctcc gtgacacaga cggtacggcg ctgagatgtc cggcccgccg
     5581 ttccacccgc cgtccggccc gtcgcgctcc acctcggccg ggcagccgga cgagcagcgg
     5641 cggcagccca cgccgtggac accgccgaac gccagcagca caccgaacgc cgagcgctac
     5701 ctgctcgagg ccgacgcact gcgacgacgt cagctgcggt ccgcactgtt gcacgggtcg
     5761 agccgaacct ggcgcgacca gggtcgcatc tggcccggcg tgatcgccgg catcgtcgtg
     5821 gtgacggtga tcctggcgtc gttcgccgtg atgaaggcgt tcgagaagtc cgaggagctg
     5881 cagaaggagc aggagagccc gaccgcaccg gccgccacct caccggcgac cccgacccag
     5941 tccacccgct gaacaccgcc tgaatcggac aggtgtcggt cggccgcgcc tgctaccggt
     6001 gcattcctca cgcgtcaagg gtcgcttcgc gatc
//
LOCUS       DATBFZ010000429         4077 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_2590942, whole genome shotgun sequence.
ACCESSION   DATBFZ010000429 DATBFZ010000000
VERSION     DATBFZ010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 4077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4077
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2590942"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="VH419_12975"
     CDS             complement(<1..294)
                     /locus_tag="VH419_12975"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEY7044575.1"
                     /translation="MRAFTAAALQVRPQPGPLTAESITANVDHCVTWVRDCVAETAAE
                     LVVLPETCTTGFTPDMGPDELWQLVDVIPGAQTRPLHDVAAELGVHLVYGAYAR"
     gene            complement(318..962)
                     /locus_tag="VH419_12980"
     CDS             complement(318..962)
                     /locus_tag="VH419_12980"
                     /inference="COORDINATES: protein motif:HMM:NF017999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase subunit G"
                     /protein_id="HEY7044576.1"
                     /translation="MQVSGSATLNASRDDVWKALTDPAVLVRTIPGCQRLETIGEDAY
                     AMTIAAGVASIKGVYTGQVRLTDQQPPDTFVLKASGAGGPGTVSADVRVKLADAGDGT
                     TSLTYDADAIVGGMIGGVGQRMLTGVAKKTAGEFFAAVDDAVNGRAAAASTTPTAGEV
                     VRETAEPSTQTVFEAPAAAVTPSPGFLQGALIGAGAALLGVIVGGWLAGRRRRT"
     gene            complement(1081..2445)
                     /locus_tag="VH419_12985"
     CDS             complement(1081..2445)
                     /locus_tag="VH419_12985"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEY7044577.1"
                     /translation="MITARAEAAHGGGSIASEDWPDGPGSRIRPQRADRELRRMLAEI
                     DPDRVERIVRKLVSFGTRHTLSSQTDPVRGIGAARDWIAAELTSYAAASGGRMTVTVP
                     SYIQQPAPRIPTPTRISDVVATLQGSVDPNRTYVITGHYDSRVTDVLNFTDDAPGADD
                     DASGVAVIMELARVMATHEPDATLIFAAVAGEEQGLFGSTFLAQQLADAGADVQGMFT
                     NDIVGSSTADDGTRDPRSLRLLAQGVPTSEDPERTATRLAIGGENDSPARELARFVTE
                     VAGRSTTGMNIRLIYRLDRYLRGGDNIPFLAQGWPAARFTEPNENFAHQHQDVRVENG
                     VQFGDLPEFCDFDFIARVARVNGAWMWSASQAPGLPQDVTIDTSVLTNDTKLDWTHGS
                     DASLAGYEVVWRPTIAPFWTHVIPVGDVDTATIDLSKDNVVFGVRAVSHDGHRSPAVF
                     PFPA"
     gene            complement(2761..3534)
                     /locus_tag="VH419_12990"
     CDS             complement(2761..3534)
                     /locus_tag="VH419_12990"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HEY7044578.1"
                     /translation="MVSAHEYGARYGRTGARASELFSDALLMSADFVEFDVRRTADGV
                     FVISHDRTVELAGGRQDVAHLTLDQLHAAGADVFTYAEALGAIRGKARAHLDLKFAPP
                     ESSYDGPDNQTWEVQATSQALAALGADNMVVTTGRDLGVAAVRRWAATDHPALLVGLS
                     LGGSKAHLPWRERFAARWSELFPAHRVKISDPNVVVANKWLALFGVARWTHRRGLLLL
                     VWTVDDHRGLRRWLGDPRCWMVTTNRVREAVRIRDGYLD"
     gene            complement(3604..>4077)
                     /locus_tag="VH419_12995"
     CDS             complement(3604..>4077)
                     /locus_tag="VH419_12995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEY7044579.1"
                     /translation="ANGMHAVVVETDPETAEITILRYCVVHDCGPIINPMILEGQVHG
                     GVAQGVGGALYEKMEYDEAGQLLNASYMDFLIPYASEVPTIDIDHLETPTPLNPLGVK
                     GAGEAGVIPGSAAIAAAIEDAEGFPINAMPISPSRLFDLRRQHHHVEPRRGGRNR"
BASE COUNT          584 a         1393 c         1465 g          635 t
ORIGIN      
        1 gcgtgcgtag gcgccgtaca cgaggtggac cccgagctcg gccgcgacgt catggagtgg
       61 tcgcgtctga gcgccgggga tcacgtcgac gagctgccag agctcgtccg ggcccatgtc
      121 tggcgtgaag cccgtcgtgc acgtctccgg cagcacgacg agctccgccg cggtctcggc
      181 gacgcagtcg cgaacccacg tcacgcagtg gtcgacgttc gcggtgatgg actcggccgt
      241 gagcggaccc ggctgcggcc gcacctgcag ggcggccgcg gtgaatgcac gcatcggcat
      301 tgatcctcct cggccggtca ggtacgccgt cggcgtccgg ccagccagcc tcccacgatg
      361 acgccgagca gcgcggcgcc ggcgcctatc agcgctccct gcaggaatcc cggactcggc
      421 gtgaccgcgg ccgccggtgc ctcgaacacc gtctgggtcg acggctcggc ggtctcgcgg
      481 accacctcac cggcggtcgg ggttgtcgaa gctgccgccg cccggccgtt gaccgcgtcg
      541 tcgactgccg cgaagaactc gcctgcggtc ttcttcgcca cgccggtgag catccgctgg
      601 ccgacgccgc cgatcatgcc gccgacgatg gcgtcggcgt cgtacgtcag gctcgtcgta
      661 ccgtcgcccg cgtcggcgag cttgacgcgc acgtccgcac tgaccgtgcc gggtccgccg
      721 gccccggacg ccttgagcac gaacgtgtca ggcggctgct ggtcggtgag gcggacctgg
      781 ccggtgtaga cgcccttgat cgaggcgaca ccggccgcga tcgtcatcgc gtaggcgtcc
      841 tcgccgatcg tctcgagccg ctgacagccg gggatggtgc ggaccaggac cgcagggtcg
      901 gtgagcgcct tccagacgtc gtcacgcgag gcgttcagcg tggccgatcc cgacacctgc
      961 atggcagcag cctagaggag cccgcggcgc gggcgagccg ccgaggtgcc aggtgttgcc
     1021 cgggacacgc cggtcctgcg gcggccggtg acacctcggc gcggctcatg cggcgtcagg
     1081 tcaggccggg aacgggaaga ccgcgggact gcggtgaccg tcgtgactga ccgcgcggac
     1141 gccgaagacc acgttgtcct tcgacaggtc gatcgtggcg gtgtccacgt cgcccaccgg
     1201 gatcacgtgc gtccagaacg gcgcgatcgt cggccgccag acgacctcgt atccggccag
     1261 gctcgcgtcg ctgccgtgcg tccagtccag cttcgtgtcg ttggtgagga cggacgtgtc
     1321 gatggtgacg tcctgcggga ggccaggggc ctgggacgcc gaccacatcc acgcgccgtt
     1381 cactctggcg acgcgggcga tgaagtcgaa gtcgcagaac tccggcaggt cgccgaactg
     1441 cacgccgttc tcgacgcgga cgtcctggtg ctggtgcgcg aagttctcgt tcggctcggt
     1501 gaaccgggcg gccggccagc cctgcgcgag gaacgggatg ttgtctccgc cccggagata
     1561 gcggtccagc cgatagatga gccggatgtt catcccggtc gttgaccgtc ccgccacctc
     1621 ggtgacgaac ctggccagct cgcgcgccgg cgagtcgttc tcaccgccga tggccagtcg
     1681 ggtcgccgtg cgctcgggat cctccgacgt gggcacaccc tgcgcgagca gccgcaggct
     1741 gcgtgggtca cgtgtcccgt cgtcggccgt ggagctgccc acgatgtcgt tggtgaacat
     1801 cccctgcacg tcggcaccgg cgtcggccag ctgctgggcg aggaacgtgg agccgaacag
     1861 gccctgctcc tcaccggcga cggcggcgaa gatcagcgtc gcgtcgggct cgtgggtcgc
     1921 catcacccgg gcgagctcca tgatcaccgc cacaccggag gcgtcgtcgt cggcgccggg
     1981 tgcgtcgtcg gtgaagttca gcacgtcggt gacacgcgag tcgtagtgcc cggtgatgac
     2041 gtaggtgcgg ttggggtcga ccgaaccctg caacgtcgcg accacgtcgc tgatccgggt
     2101 cggcgtgggg atgcgcggag cgggctgctg gatgtacgac ggcaccgtca ccgtcatccg
     2161 cccgccggac gccgcggcgt acgacgtcag ctcggcagcg atccagtccc gggccgcccc
     2221 gatgccgcgg acggggtcgg tctgcgatga cagcgtgtgg cgggtgccga aggagacgag
     2281 cttgcggacg atgcgttcga cccggtcggg gtcgatttcg gccagcatcc tgcgcagctc
     2341 ccggtccgcg cgttgcgggc ggatgcgcga accaggaccg tccggccagt cctcgctggc
     2401 gatgctgccg cccccgtggg ccgcctcggc acgtgcggtg atcaactgcg agccgacagc
     2461 gccggcagcg agagcgccag acccggcgag gaatgctcgg cggctggcgc gtggagatgg
     2521 ggccgaggat ggggccgagg atgggtggag tcggaaatcg ttcatggcgc cttcctgggg
     2581 tgtgggtgcg tgcgccggta atgggcacaa cacttccacc gacttaccgg tcccgcatct
     2641 gcccatccgg gcatccaccc ccgccgaggt ggcaactatc gttgcatttt gacggcgtgt
     2701 cggacgtaca tcgccacttc gcccgcaggg ccgcggtcgg ttccccgtcc ggtatgccga
     2761 tcagtccagg tagccgtcgc ggatgcgcac ggcttcacgg acgcggttgg tcgtcaccat
     2821 ccagcacctc gggtcgccca gccaccgtcg cagcccacgg tggtcatcga cggtccagac
     2881 gagcagcagc aggccgcgcc ggtgtgtcca ccgggcgaca ccgaacagcg ccagccactt
     2941 gttggcgacc acgacgttgg ggtcactgat cttgacgcga tgggcgggga acagctccga
     3001 ccacctggcg gcgaaccgct ctcgccaggg gaggtgggcc ttcgatccgc ccagtgagag
     3061 gccgaccagc agcgctggat ggtcagtggc ggcccatcgc cgaaccgcgg ccaccccgag
     3121 gtcgcgcccg gtcgtcacga ccatgttgtc ggcgccgagc gcggccagcg cctggctcgt
     3181 cgcctgcacc tcccacgtct gattgtctgg accgtcgtac gacgattccg gtggcgcgaa
     3241 cttgaggtcg aggtgcgcac gggccttgcc ccggatcgcg ccgagcgcct ctgcgtaggt
     3301 gaacacgtcc gcgccggcag catgcaactg gtcgagcgtg aggtgcgcga cgtcctgccg
     3361 gccgccggca agctccaccg ttcggtcgtg gctgatgacg aagacgccgt cggccgtgcg
     3421 gcgtacgtcg aactcgacga agtctgccga catcagcagc gcgtcggaga acagctcgga
     3481 ggcccgagcc ccggtgcggc cgtaccgggc gccgtactcg tgtgcagaca ccatcgccac
     3541 ggccagatcc tgccaagccc ggagtgccga ggtggcaagc gcgtgccgac acgccgtcaa
     3601 agttcaacga ttcctgccac ctcggcgggg ttcaacgtgg tggtgttggc ggcggaggtc
     3661 gaagaggcgg ctcggggaga tcggcatcgc gttgatcggg aagccttcgg cgtcctcgat
     3721 ggcggcggcg atggctgccg agccgggaat gaccccggcc tcaccggcgc ctttcacgcc
     3781 gagcgggttg agcggggtcg gcgtctcgag atggtcgatg tcgatcgtcg ggacctcgct
     3841 ggcgtacggg atgaggaagt ccatgtagga ggcgttcagc agctgcccgg cctcgtcgta
     3901 ctccatcttc tcgtagagcg ccccgccgac gccctgcgcg acaccaccgt gcacctggcc
     3961 ctcgaggatc atcgggttga tgatcggacc acagtcgtgc acgacgcagt agcgcaggat
     4021 cgtgatctcc gccgtctccg ggtcggtctc gacgacgacc gcgtgcatgc cgttggc
//
LOCUS       DATBFZ010000430        16311 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_2590992, whole genome shotgun sequence.
ACCESSION   DATBFZ010000430 DATBFZ010000000
VERSION     DATBFZ010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 16311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16311
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2590992"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="VH419_13000"
     CDS             <1..280
                     /locus_tag="VH419_13000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044580.1"
                     /translation="SPYNETHWPPKSGDGSNFEDLYKQAAAELDDGKRNDIMHQMQQY
                     EYDIGGYIIPFFNNLIDGYAANVNGFVPSKGTLNLDSFGRGFRVMWFS"
     gene            296..1270
                     /locus_tag="VH419_13005"
     CDS             296..1270
                     /locus_tag="VH419_13005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009078713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY7044581.1"
                     /translation="MRWLRHGGIAAFIVRRLLLGLLVMFLVSVLVFVATQALGDPARA
                     ILGRDATPDRLAALQDQLNLKSSVVAQYFSWLGGLLHGDLGISLAAQQPVTELLGPRL
                     VNTAVLVFCAAVISIPISIAVGSWAALRREKAFDAVSSNLLLLLAALPEFVVAVLLVM
                     LFATTVFQNALPAVSSVPPGQRPWDDPKILILPTLTLVLAVAPYVARIMRASMIEVLE
                     SDYVEMARLKGLQERTVLVRHALPNAIGPVFQVIAINLAYLAGGVIVVEYVFNYPGVG
                     SALQDSVVNHDIPVVQALAMLIAAVYVILNLLADVATILVTPKLRTRL"
     gene            1267..2190
                     /locus_tag="VH419_13010"
     CDS             1267..2190
                     /locus_tag="VH419_13010"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY7044582.1"
                     /translation="MSIEPSMSTSDEVKLLAAYETGIPAGHDASLLRRALRLWRSRIG
                     LGCMLLVVALAVVGPFVAPHDPDEFVGPPFSARGTGNGLFGTDYLGQDVWSRFLHGGY
                     GILTTAVISTAIGLVVGIIIGLVAAYARNALDDVLMRAMDVIMAFPQIMLALVAVSLL
                     GAHTWLIIAAIALTTAPRVARVARGSAQPVVERDFIAATESMGLSRFRILIGELFPNI
                     LGPMMVEASLRLTYSITVIASLAFLGLAPDQNSSDWGSMIQQNRLALATQPWGALLPI
                     LGIAVLTMGTGLFGDGIARTAAGIDRERGDE"
     gene            2187..4040
                     /locus_tag="VH419_13015"
     CDS             2187..4040
                     /locus_tag="VH419_13015"
                     /inference="COORDINATES: protein motif:HMM:NF008453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY7044583.1"
                     /translation="MTSHDPLGQAAPAAVSVRDLEISTTSGVPVVTEVSFEIAAGEVL
                     GVVGESGSGKTTVGLSLLGHARRGLRITNGSIMLGEKSILDMDEDALRSLRGSLVSYV
                     PQDPASSLNPALRIGLQLRETLAAHGVSSSKERDARIAEMMREVALSDDPKYLQRYPH
                     ELSGGQQQRIGLAMAFANRPRLIVLDEPTTGLDVTTQATVLATVRDLAAAHGVGALYV
                     SHDLAVVAAIARRVAVMYAGRIVELGPAEELFDSSAHPYTRRLVGAIPRLSGGRSLVG
                     IPGHAPSPGRRPPGCAFAPRCRLHIDDCDKQVPDLVLVAPEHRARCIRAQEVIGQTQA
                     KVGDPVDVAVGDESSVLTLENVVAHYGKIEVLHSINLALQAHECLAVVGESGSGKTTT
                     ARSIAGLHRQWTGSIKLAGQELEQAARARSTETRRQIQYIFQNPYGSLNPRRTIGDTV
                     GQPLSVFRIASGRKADQLVGEMLEQVSLSASYARRYPDQLSGGERQRVAIARALISKP
                     SILICDEVTSALDVSVQAAIVELLGSLQRELGLSMVFVTHNLPLVRSIAQQVAVLADG
                     NIVEYGETAEILANPQQVYTQELIANTPSLEVAAAEAAGEMPTPGHVPTPN"
     gene            4086..5654
                     /gene="betC"
                     /locus_tag="VH419_13020"
     CDS             4086..5654
                     /gene="betC"
                     /locus_tag="VH419_13020"
                     /EC_number="3.1.6.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR03417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline-sulfatase"
                     /protein_id="HEY7044584.1"
                     /translation="MSPSRSPDILVVMADQLAPAALPFHGDPVTRAPAMSELADRGVV
                     FDAAYTGSPLCAPARAALLTGRLPSGTGAYDNAAGFASDVPTFAHYLRRAGYRTALAG
                     KMHFCGPDQLHGFERRLTTDIYPADYGWTPDWTRPEYRPHWYHDMSSVVDAGPCVRSN
                     QLDYDEEVGLAAEQALYDHVRSDDERPLCLVASFTHPHDPYTISQPWWDLYDGVDIPM
                     PAFGYDDAVATAHERRLRQVCAMDDIEVTHEHVRAALRAYRGAISYVDQQVARLLDAL
                     RSTGRLEDTVVVLTSDHGDMLGERGLWYKMCFFEGAARVPLVIAAPDRFAPARVAAPV
                     STLDLLPTLVAVANNGDGSDVVGQIDGRSLYPMLAGDSVDRGDVVAEYLAEGAIAPMV
                     MIRRGDLKLVHSPADPDQLYDLSADPLERWNLASEPGHASPLAQLRGEVAARWNLSEL
                     DHRVRESQRRRTTIVEALARGESVSWDYAPPYDAARRYVRNEKDLGEFEAASRFPRLP
                     PPAPTGAECRTQPE"
     gene            complement(5642..5797)
                     /locus_tag="VH419_13025"
     CDS             complement(5642..5797)
                     /locus_tag="VH419_13025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044585.1"
                     /translation="MTARTAMAPTSHRGVSKMSRLSTVVGRAWNDARTMDRRLMEIRT
                     GLTRHSG"
     gene            5912..6841
                     /locus_tag="VH419_13030"
     CDS             5912..6841
                     /locus_tag="VH419_13030"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY7044586.1"
                     /translation="MLDVHRLDVLQRFAAHGSIAGAAAELGCSPSAVSQQLATLEREA
                     GLPLIERTSRTASLTDAGRELVEHASIILSAVETAQTRMRARMGVIAGRVRVSFIPGL
                     AATVAPDLAELQRRHPALTVVALQIESTAAPTALLDRATDVAVIDEWGEESVASSTAL
                     RVHRAHREPIVLAVPAEHPIAHSHHAKPVSATTLRDLVAHETWLSTPAGQVSRLAGDA
                     RLTAIGATPSRRWQFDGLYVLARLVAVGSGIALLPSSIAAYERKVAAIPLRPPMYRYV
                     RILTRSTTQDDPAIAACVKAVRQALTRRPARGT"
     gene            complement(6891..7685)
                     /locus_tag="VH419_13035"
     CDS             complement(6891..7685)
                     /locus_tag="VH419_13035"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEY7044587.1"
                     /translation="MSEPVRTQRAEHEDSVLVITLDRPKANAIDVATSHALHDAFRRL
                     EDDPSLRVGVLTGAGSRFFSAGWDLHAAAEGEAVDADHGPGGFAGLTELWARTKPLIA
                     AVNGLAMGGGCELALAADLIVMADDAEIALPEVRLGLLPDAGGVLRLPRRLPGSVARE
                     MLLTGRRMGAAEAFRWGLANRVVPRDELMSAALDLAHRICESAPLAIAAVLEILAVTS
                     GGGVREGFDVLRSGDLPHYEAMLRSEDAVEGPRAFAEKRAPQWRGR"
     gene            complement(7699..8859)
                     /locus_tag="VH419_13040"
     CDS             complement(7699..8859)
                     /locus_tag="VH419_13040"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY7044588.1"
                     /translation="MDFGLDDEQRAIVDTVRAFVTKELYPHEDEVERLGDVPPELVEQ
                     IRKKALTAGLYAANMPAELGGGGLDAVGITLVERELGRASYALQWLVARPSNILLACE
                     GEQRDRYLLPAIRGERHDCLAMTEPDAGSDVRSMTTRARRDGDEYVIDGTKHFISNAD
                     KSDFAIVFAATRTEETTQGSRRSITGFLVDLDATGLTVRRGSGSVSHRGYHHSELAFE
                     GCRVPAANRLGEEGRGFELMGEWLGASRLTVAASSVGRARRVLEMAIQWAAERVQFGQ
                     PIGRFQGVGFQLADMATELEAAELLTLRAAWRLDHATMSDQDAAMAKVFATEALGRIT
                     DKAIQIFGGMGLMDELPIARFWRDARVERIWDGTSEIQRHIIARSMLRPLGA"
     gene            complement(8878..9417)
                     /locus_tag="VH419_13045"
     CDS             complement(8878..9417)
                     /locus_tag="VH419_13045"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEY7044589.1"
                     /translation="MDDRVVTPYEGTRHATWDARAPIDAPLRLHRTVVAAQWVDYNGH
                     MSESCYLLVFGDSADAFFRFIGIDDDYRTAGHSLYTAETHLHHRREVAEGEPVACSLQ
                     LLDHDDRRLHIFHEMTHGESGMSLAAAEQLLVHVDMAAGRATPMPSILLDRLIAIRQA
                     HAALRTPDVVGRPMGIRRG"
     gene            9664..10083
                     /locus_tag="VH419_13050"
     CDS             9664..10083
                     /locus_tag="VH419_13050"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY7044590.1"
                     /translation="MTDSTDHGREPWVTDIEAATLDNTDFRSTLWTGEHLQLTVMSIP
                     VGGDIGLEVHPDNDQFLRIEQGRGRVQMGTEKDDLAFDREVEDDWVILVPAGHWHNVT
                     NIGDEPLKVYALYGPPDHKPGTVHHTQQDALNDPDEH"
     gene            complement(10135..11184)
                     /locus_tag="VH419_13055"
     CDS             complement(10135..11184)
                     /locus_tag="VH419_13055"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEY7044591.1"
                     /translation="MLITFRDMQWRARRLVLGLAAISLVLGMTALLGALHDGFLRETD
                     RTITFFDADTWIVPTDVSGPFTANSPLRQTAVDAVRAEPGTEEVTPVAIFRHVVDGVG
                     NGFTDVNVIAYQPDGVVAPATTSGRAPSRVGEAAVDVHLGVEVGDTLEIAGRRLTVVG
                     QVRGLTYNGGTPTVLITLRDGQSIAFDGRRLASALVVRGNPEALPDGLAAMTPTQVRD
                     DLRRPLSVATTAIGIVAGLLWLVAAGIAGMLSFLSGLDRHRDFAVYKAYGVSTHRLLT
                     SMMSEGAIISLVAGGVALVLAYALIPLFPVSISLTGADCLRLVGLALVAGVASSMLSV
                     RGVVRVDPAVAFTNS"
     gene            complement(11184..11906)
                     /locus_tag="VH419_13060"
     CDS             complement(11184..11906)
                     /locus_tag="VH419_13060"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEY7044592.1"
                     /translation="MSTWSTRRPGAADVLTLCNALCGAAAILVICWDEPVAALDVGQR
                     YEVAALLFVCGTVFDVLDGAAARRWGGTPLGGPLDCLADGVTFGVAPVVAVTAYVTSG
                     SSSAEQLVVAAGALAYVAAALVRLADFMAHYRERSDFVGLPTTSACVAAVCLGFLSPP
                     PALAGVGLAALAFLMVSPLAYPAAARVLAVCLAGWALGVMGIIGVLDVRVPATLALLT
                     ITLVVPVTTHIRARAVAASASS"
     gene            complement(11903..12526)
                     /locus_tag="VH419_13065"
     CDS             complement(11903..12526)
                     /locus_tag="VH419_13065"
                     /inference="COORDINATES: protein motif:HMM:NF014696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HEY7044593.1"
                     /translation="MTVRLARGTPGFVALLWFAPLVVASRWARLVVLLSAAGCTWFFR
                     DPRRTPKGEGLLAAADGVVQWVRTDERGRETISTYLNLLDVHVTRAPCDATVVQQTYR
                     RGRHRPAFSPSAHRNERMEWTLATAHGDIVLIQYAGTVARRIVTHKRTGDVLRRGEAI
                     GLIRFGSRVDVVLPVGVSAAAGEGDRMYGGATVIATPDVMTQPSGLT"
     gene            complement(12523..13230)
                     /locus_tag="VH419_13070"
     CDS             complement(12523..13230)
                     /locus_tag="VH419_13070"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY7044594.1"
                     /translation="MRGEVEHVSPYLSVDGLTVEYTLGSHRSRVLENVSFEAGRDEIV
                     LALGPSGSGKTTLLSVLAGLLRPASGRVILDGRDVIDLEGRDLLAYRRNNVGIVFQTF
                     NLVPSLSALENVMAPLVLTGVRSAVARRTARRLLDEVDLADHAHQRPGTLSGGQQQRV
                     AFARALVNNAPVILADEPTAHLDPVQVDAILRLIRGLRGQGRLVVVATHDARFEPLAD
                     RVVSLGQHSAVGTIEAR"
     gene            13467..14633
                     /locus_tag="VH419_13075"
     CDS             13467..14633
                     /locus_tag="VH419_13075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009076353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEY7044595.1"
                     /translation="MLVALRRDGLDPELAGAADPITKVILPLGIRGWLVTGYDEVRQV
                     LADHDSFSNDFGNLVGRAGVAAGLDPGGLGFTDPPYHTRMRHMLTRHFTTRRLAALAP
                     RVSAIVESSLADVERELDAFGRTDLVERFAIPVPTLTICELLGVPYADREHFQQLSSA
                     RFDLTGGASASLDAVNESLTYLEDLVARRRREPDDGLLAGLVADHGSQFSDRELAGVA
                     DGLLTGGIETTVSTLGLGSLVLLQDPVSFARLAQDEVFAAAYVEELLRYLTVVQVAFP
                     RFARRDVDVGASLVRRHDVVLCSLSAAGRDPRTGEHLNEVDPEAARRPHLAFGHGIHR
                     CVGAELARLELRLAFPALSRRLPDLRLAVDTSELEYRALSIVFGLPSLPVTCSR"
     gene            14675..15379
                     /locus_tag="VH419_13080"
     CDS             14675..15379
                     /locus_tag="VH419_13080"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY7044596.1"
                     /translation="MDISLCLDTARPWSELLELARRADDEALYAIYVPDHFGRHREAW
                     TLLSALAASTHRVRLGVLVLGVTHRHVGLLGEMARTLGEVSNGRLVLGLGAAWDEGEH
                     RSLGLAFPGPGERLDLLEAALGQLVDVGPPLLVGGRGERRTMPIAARYASIWHSWAAP
                     DEFQRKCGIMDRLCRDIGREPSAVRRATGGVLLDEYDSAGSLAAYRRAGVDEFIVRDH
                     RDASVTDSLAALPNQR"
     gene            15502..16074
                     /locus_tag="VH419_13085"
     CDS             15502..16074
                     /locus_tag="VH419_13085"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009076517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY7044597.1"
                     /translation="MGTNDSETDLGITLLEPAQSRDEHLVVRLTSLINRVYETAESGL
                     WHDSAARTTKSELAALIEERQIAVATREGEIVGSVQIHDVSTDISEFGMLVADPDHRD
                     TGVGRALVDYAEGRSRERGLRAIQLELLVPRTWRHPSKEFLKEWYGRRGYRLVGTTTI
                     EHTHPHLAPLLATPCDLTIYEKPLQATDVG"
     gene            complement(16102..>16311)
                     /locus_tag="VH419_13090"
     CDS             complement(16102..>16311)
                     /locus_tag="VH419_13090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044598.1"
                     /translation="HDTQYLVNALAQLGSPPNNPDAWVRALQDLQQAVEMLNLLGPDD
                     WRVNIMAAANEFQAMGIQACGAIFG"
BASE COUNT         2648 a         5692 c         5367 g         2604 t
ORIGIN      
        1 ctcgccgtac aacgagacac actggcctcc caagtccggc gacggatcga acttcgagga
       61 cctctacaag caggcggccg ccgagctcga cgacggcaag cggaacgaca tcatgcacca
      121 gatgcagcag tacgagtacg acatcggcgg ctacatcatc ccgttcttca acaacctcat
      181 cgacgggtac gccgcgaacg tcaacgggtt cgtgccgagc aaggggacac tgaacctcga
      241 cagcttcggt cgcggcttcc gcgtcatgtg gttcagctga gcgacagggc gtccgatgcg
      301 ctggctgaga cacggtggga tcgcagcctt catcgtcagg aggctgctgc tcggcctgct
      361 ggtgatgttc ctggtgtccg tgctcgtctt cgtcgcgacc caggcgctcg gagacccggc
      421 acgcgcaatc ctcgggcggg atgcgacacc ggaccggctg gctgcgttgc aggatcagct
      481 caacctcaag agttcggtgg ttgcgcagta cttcagctgg ctcggcggac tcctgcacgg
      541 tgacctcggc atctcgttgg ctgcgcagca gccggtgacc gagctgctcg gaccacgcct
      601 cgtcaacacc gcagtgctgg tcttctgcgc cgcggtcatc tcgatcccga tctcgatcgc
      661 cgtcggctcg tgggccgcac tgcgacgcga gaaggccttc gacgccgtct cgtcgaacct
      721 gctgttgctg ctggccgcgc tccccgagtt cgtggtggcc gtgctgctgg tgatgctctt
      781 cgcgaccacg gtcttccaaa acgcgctgcc agccgtcagc tcggtgccgc caggccaacg
      841 gccgtgggac gatccgaaga tcctgatcct gcccacactg acgctggtgt tggcggtcgc
      901 gccctacgtc gccaggatca tgcgcgcgtc gatgatcgag gtgctcgaga gcgactatgt
      961 cgagatggcg cgcttgaagg gtcttcaaga gcggacggtg ctcgtacggc atgcgctgcc
     1021 gaacgccatc ggtccggtgt tccaggtcat cgccatcaac ctggcgtacc tcgccggcgg
     1081 tgtcatcgtc gtcgagtacg tgttcaacta ccccggagtc ggcagcgccc tccaggactc
     1141 ggtcgtcaac catgacatcc cggtcgtcca ggcgctcgcc atgttgatcg cggccgtcta
     1201 tgtcatcttg aacctgctcg ccgacgtcgc gacgatcctc gtgaccccga agctgaggac
     1261 ccggctatga gcatcgaacc gagcatgagc acgtccgacg aagtcaagct gctggcggcg
     1321 tacgagacgg ggataccggc gggtcatgac gcgtcgctgc tgcgccgcgc actgcgactg
     1381 tggcgaagtc ggatcggtct cggctgcatg ctgctggtcg tcgccctcgc ggtggtcggc
     1441 ccgttcgtcg ccccgcacga ccccgatgag ttcgtgggcc cgccgttctc ggcgcgcggg
     1501 accggcaacg gcttgttcgg taccgactac ctcggacagg acgtctggag ccgtttcctg
     1561 cacggtggct acggcatcct caccactgcg gtgatctcca cggcgatcgg tctggttgtc
     1621 ggcatcatca tcggcctggt cgccgcctat gcacgcaacg cgctggacga cgtcttgatg
     1681 cgggcaatgg acgtgatcat ggcgttcccg cagatcatgc tcgccctggt tgccgtcagc
     1741 ctgctcggtg cgcacacctg gttgatcatc gcagcgatcg cgctcaccac ggccccgcgc
     1801 gtcgcgcggg tggcccgtgg ctccgcccag ccggtcgtcg agcgcgactt catcgccgcg
     1861 accgagtcga tgggcctctc gcgtttcagg atcctcatcg gcgagctgtt ccccaacatc
     1921 ctgggcccga tgatggtcga ggcgagcctt cggctgacat actccatcac cgtcatcgct
     1981 tcgttggcgt tcctgggatt ggcgccggat cagaacagct ccgactgggg gtcgatgatc
     2041 cagcagaacc ggctcgcgct ggcgacgcaa ccctgggggg cgctgctgcc gatcctgggc
     2101 atcgccgtgc tcacgatggg caccggcctg ttcggtgacg gcatcgcccg caccgccgcg
     2161 ggtatcgacc gtgaacgagg tgacgagtga ccagtcacga cccactcggc caggccgcgc
     2221 ccgccgccgt gagcgttcgc gatctggaga tctccaccac ttctggtgtt cccgtcgtca
     2281 cggaggtctc cttcgagatc gcggccggcg aggtcttggg cgtcgtgggc gagtccggat
     2341 cgggcaagac caccgttgga ctgtccctcc tcggtcatgc gcgccgcggc ctgaggatca
     2401 ccaacgggtc gatcatgctc ggcgagaaaa gcatcctcga catggacgag gatgcgctcc
     2461 ggtccctgcg gggctcgctc gtctcgtacg tgccccaaga ccctgcgtcg tcgctcaacc
     2521 cggcgctgcg gatcggcctg cagctgcgcg agacgctcgc ggcgcacggc gtctcgagca
     2581 gcaaggagcg cgatgcgcgg atcgccgaga tgatgcgcga ggtcgcattg tcagacgatc
     2641 cgaagtacct gcagcgctac ccacacgaac tttccggcgg tcagcagcag cgcatcggcc
     2701 tcgccatggc cttcgccaac cggccgcgcc tgatcgtgct cgacgagccg acgaccggtc
     2761 tcgacgtgac gacgcaggcg actgtgctcg ccaccgttcg cgatctggcc gccgcacatg
     2821 gggtgggtgc gctctacgtc agccacgacc tcgccgtggt cgccgccatc gctcgccgtg
     2881 tggcggtgat gtatgccggt cggatcgtcg agctcggacc ggccgaagag ctgttcgact
     2941 cctctgccca cccctacacg cgtcgactgg tgggcgcgat cccgcgcctt tcgggagggc
     3001 gcagccttgt gggcatcccc ggtcacgccc catcacccgg gcgtcggcca cctggctgcg
     3061 cgttcgcgcc ccggtgcagg ctgcacatcg acgactgcga caagcaggtc ccggacctgg
     3121 tgctggtggc tcccgagcac agagcgcgct gcatccgtgc gcaggaggtc atcggtcaga
     3181 cccaggcgaa ggtcggtgat ccggtcgacg tcgccgtcgg cgacgagtcg tcggtcctca
     3241 cgctcgagaa cgtcgtcgca cactacggca agatcgaagt cctgcactcc atcaacctgg
     3301 cgctgcaggc gcacgaatgt ctcgctgtcg tgggtgagtc cggatcgggc aagaccacca
     3361 ccgcccgttc gatcgcgggc ctgcaccgtc agtggaccgg gagcatcaag ctcgccggcc
     3421 aggagctcga gcaagcggct cgcgcccgct ccaccgagac ccgtcgacag atccagtaca
     3481 tcttccagaa cccgtacggg tcgctcaacc cgcgaaggac gatcggcgac accgttggtc
     3541 agcccctctc cgtcttccgc atcgcgtccg ggcggaaggc tgaccagctc gtcggcgaga
     3601 tgctcgagca ggtgtccctg tcggcgtcgt acgcccgccg gtatcccgac cagctctcgg
     3661 gtggggagcg ccaacgtgtc gccatcgccc gcgcgttgat ctcgaagccg tcgatcctga
     3721 tctgtgacga ggtcacctct gccctcgacg tcagcgtgca ggcggcgatc gtcgagctgc
     3781 tcggaagcct gcagcgcgag ctcgggctgt cgatggtctt cgtcacgcac aacctcccgc
     3841 tggtgcgttc gatcgcgcag caggtcgcgg tactcgccga cggcaacatc gtcgagtacg
     3901 gcgagacggc ggagatcctc gcaaacccgc aacaggtgta cacgcaagag ctgatcgcca
     3961 acaccccgtc gctcgaggtc gctgcggccg aggcggcggg cgagatgccg accccggggc
     4021 acgtaccgac ccccaactga gaccaggcag gacctgtctg accgaagacg gaggtcagcg
     4081 ccgacgtgag cccttcgcgc agtccggaca tcctcgtcgt gatggccgat cagctggcgc
     4141 cggccgcgct gcccttccac ggggacccgg tgacacgagc accggcgatg tcggagcttg
     4201 cagaccgcgg tgtcgtcttc gacgcggcgt acaccgggag cccgctctgc gccccggcgc
     4261 gggctgcgct cttgacggga cggctgccgt caggcaccgg cgcgtacgac aacgcggcag
     4321 gcttcgcctc tgacgtcccg acgttcgcgc actacctgcg tcgggctgga taccgcacag
     4381 cgctcgcggg aaagatgcac ttctgcgggc ctgaccagct gcacgggttc gaacggcggc
     4441 tcaccaccga catctatccg gccgactacg ggtggacgcc cgattggacg cgaccggagt
     4501 accggccgca ctggtaccac gacatgtcgt cggtcgtcga tgccgggccg tgtgtgcgca
     4561 gcaaccagct cgactacgac gaggaggtgg gcctcgccgc cgaacaagcg ctgtacgacc
     4621 atgtgcgctc cgacgacgag cggccgctgt gtctcgtcgc gtcgttcacg cacccgcacg
     4681 acccgtacac gatctcgcaa ccgtggtggg atctctacga cggcgtcgac atcccgatgc
     4741 cggcattcgg gtatgacgac gcggtcgcca cagcgcacga gcgacggctt cgtcaggtct
     4801 gcgccatgga cgacatcgag gtcacgcacg aacacgtccg ggctgcgctg cgggcctacc
     4861 gcggcgccat ctcgtacgtc gaccagcagg tcgcacggtt gctcgatgcg cttcgcagca
     4921 cgggtcggct cgaggacacg gtggtcgtgc tcaccagtga ccacggcgac atgctgggcg
     4981 aacgtgggct ctggtacaag atgtgcttct tcgagggggc cgcacgtgtt ccgctcgtca
     5041 tcgcggcgcc ggaccggttc gcacccgccc gggtggcggc gccggtgtcg acgctcgacc
     5101 tgctacccac cttggtggcg gtggcgaaca acggggacgg gtcggacgtc gtcggccaga
     5161 tcgatggccg gagcctctac ccgatgctcg cgggtgactc cgtggaccgc ggcgacgtgg
     5221 tggcggagta cctcgccgag ggtgcgatcg cgcccatggt catgatccgt cgtggcgacc
     5281 tgaagctcgt ccactccccc gccgatccgg accagctcta cgacctgtcg gccgacccgc
     5341 tggagcgctg gaacctggcg agcgagccgg ggcacgcgag ccctctcgcc cagctgcgcg
     5401 gtgaggtggc ggcacgctgg aacttgtcag agctcgatca ccgagtacgg gagagtcaac
     5461 ggcggcgaac cactatcgtc gaggcgctgg cgaggggcga gtcagtgtcg tgggactacg
     5521 cccctccgta cgacgcagcg cgacgttacg tgcgcaacga gaaggatctc ggcgagttcg
     5581 aggcagcgtc acgctttccg cgcttgccgc ctccagcgcc cacgggagct gaatgcagga
     5641 ctcagccgga gtgacgcgtc aggccggtcc tgatctccat cagccggcgg tccatcgtgc
     5701 gcgcgtcgtt ccaggcccgg ccgacgaccg tcgaaagacg cgacatcttc gagactccac
     5761 ggtgagacgt gggcgccatg gcagtgcggg cggtcatgct gttcgatccc tccgaaactg
     5821 tcgcgaggcc gtctcacgac acgccaagtg tggcaggctg gctaccatca gctcaagctg
     5881 aagttagccg agggtagggt aagcaaacct gatgctcgat gttcaccgtc tggacgtgct
     5941 ccagcggttc gccgcgcatg gcagcatcgc gggcgcagcc gccgaactcg gctgttcgcc
     6001 gtccgctgtc tcacagcagc tcgccaccct cgagcgggag gctggactcc cgctcatcga
     6061 acggacctca cgcacggcca gcctcaccga tgcaggccgt gaactggtcg agcacgcctc
     6121 gatcatcttg tccgccgtcg agacagcgca gacgcggatg cgggccagga tgggcgtcat
     6181 cgccggacgt gtccgggtca gcttcatccc gggcctggct gcgaccgtcg cccctgacct
     6241 cgcggagctg cagcggcgcc atcccgcact cacggtggtc gcgctgcaga tcgagtcgac
     6301 cgctgccccg accgccctgc tcgaccgggc gacggacgtg gctgtgatcg acgagtgggg
     6361 cgaggagtcg gtggcctcgt ccaccgcgct gcgggtccac cgggcgcatc gtgagccgat
     6421 cgtcctcgcg gtccctgccg agcacccgat cgcgcacagc caccatgcca aaccggtgtc
     6481 cgcgacgacg ctgcgcgacc tcgtcgcaca cgagacatgg ttgagcaccc cggcagggca
     6541 ggtttcgcga ctcgctggtg acgcacggct caccgcgatc ggtgccacgc cgtcgcgccg
     6601 ttggcagttc gacggtctct acgtgctcgc caggttggtt gctgtggggt caggcatcgc
     6661 gctcctgccc tcgagcatcg cggcgtacga acgcaaggtc gccgccatcc ccttgcggcc
     6721 gccgatgtac cggtacgtgc gaattctcac ccggagtacg acgcaggatg atccggcgat
     6781 cgccgcctgc gtcaaagcag tgcggcaggc gttgacccgg cgtcccgcac gagggacgtg
     6841 agagcagaca gcacgagggc gtacggcaag tggtgcccag ccgcacaggt tcaacgacct
     6901 ctccactgtg gcgcgcgttt ctctgcaaag gcccgcgggc cctcgacggc atcctccgac
     6961 cgcagcatcg cctcgtagtg cggcaggtcc cccgaccgca gcacgtcgaa gccttctctg
     7021 acgccacctc ctgatgtgac cgcgagaatc tcgagcaccg ccgcgatcgc aagcggagcg
     7081 gactcgcaga tgcggtgcgc caggtcgagc gctgcggaca tcagctcgtc gcgcggcacg
     7141 acccgattgg ccagccccca gcgaaacgcc tccgccgcgc ccatgcggcg accggtgagc
     7201 agcatctctc gcgccacgga ccccggcagc cgtctcggca gccggaggac gccgccggcg
     7261 tcgggcagca ggccgagccg cacctccggc agcgcgatct cggcgtcgtc cgccatcacg
     7321 atcaggtcgg cggcgagggc gagctcgcac ccaccaccca tggcgagccc gttgacggct
     7381 gcgatcagcg gcttcgtccg cgcccacagc tcggtgaggc cggcaaaacc gccggggccg
     7441 tggtcggcat cgaccgcctc cccctcggca gcggcgtgga ggtcccaacc ggcggagaag
     7501 aaccgcgaac ctgcgccggt cagtaccccg acccgcaacg aagggtcgtc ctcgagccga
     7561 cggaacgcgt cgtgaagtgc gtggctcgtg gcgacgtcga tcgcgttcgc cttgggcctg
     7621 tcgagggtga tgacgaggac gctgtcctcg tgttctgcgc gctgtgtacg aaccggctcc
     7681 gacaaatgcg gcctccgctc aggcaccgag cggtcgcaac atcgaccgcg cgatgatgtg
     7741 tcgctggatc tcgctggtgc cgtcccagat acgctcgacc cgcgcatcac gccagaaccg
     7801 cgctatcggc agttcgtcca tcaggcccat cccaccaaag atctggatcg ctttgtcggt
     7861 gatccgcccg agcgcctcgg tcgcgaacac cttggccatc gccgcgtctt ggtcactcat
     7921 cgtcgcgtgg tcgagacgcc acgcggcacg cagcgtcaac agctcggccg cttcgagctc
     7981 ggtcgccatg tccgcgagct ggaagccgac gccctggaac cgtccgatcg gttgtccgaa
     8041 ctgcacgcgc tcagccgccc actggatcgc catctccagg acacggcgtg cccggccgac
     8101 gctgctggcg gcgaccgtga gcctgctcgc gccgagccac tcccccatca gctcgaagcc
     8161 gcggccctcc tcaccgagcc ggttcgccgc cggcacccga cagccctcga acgccagctc
     8221 gctgtggtgg tatccgcggt gcgacacgct gccagagccg cgccggacgg tcagccccgt
     8281 ggcgtccagg tcgacgagga agccggtgat gctcctacgc gacccctgcg ttgtctcctc
     8341 ggtccgggtg gccgcgaaca cgatcgcgaa gtcagacttg tcggcgttgc tgatgaagtg
     8401 cttcgtgccg tcgatgacgt actcgtctcc gtcgcgccgc gcccgcgtgg tcatcgaccg
     8461 gacgtcggat ccggcgtccg gctccgtcat cgccagacag tcgtgccgtt cacccctgat
     8521 cgcgggtagc aggtagcggt cgcgctgctc gccctcgcat gccagcagga tgttgctggg
     8581 ccgggcgacc agccattgca acgcgtagct ggcccgaccg agctcacgtt cgaccagcgt
     8641 gatgccgacc gcgtcgagcc ctccgccgcc cagctctgcg ggcatgttgg cggcgtacag
     8701 acccgccgtg agcgccttct tgcggatctg ctcgacgagc tccggcggca cgtcgccgag
     8761 acgctccacc tcgtcctcgt gcgggtacag ctccttggtg acgaatgctc gaacagtgtc
     8821 gacgatcgcc cgctgctcgt cgtcgagccc gaagtccatc gtgtctgcac cttcctctca
     8881 gccgcgccgg atgcccatcg gtctgcccac cacgtcgggc gtgcggaggg cagcgtgggc
     8941 ctgtcggatc gcgatgaggc ggtcgaggag gatgctcggc atcggcgtcg cacggccggc
     9001 cgccatgtcc acgtgcacga gcagctgttc ggccgcagcc agcgacatcc ccgactcacc
     9061 gtgtgtcatc tcgtggaaga tgtgcaggcg tcggtcgtcg tggtcgagca gttggagcga
     9121 gcacgcgacc ggctcaccct cggccacctc gcggcgatgg tgcaggtgtg tctcggcggt
     9181 gtagagcgaa tgcccggcag tgcggtagtc gtcgtcgatg ccgatgaagc ggaagaacgc
     9241 atccgcgctg tccccgaaga ccagcaggta gcaggactcg ctcatgtgcc cgttgtagtc
     9301 gacccattgg gcagccacga ccgtgcgatg cagccgcagc ggcgcgtcga tcggagcgcg
     9361 cgcatcccag gtcgcgtgac gcgtgccttc gtacggcgtc acgaccctgt cgtccatcgg
     9421 gttcctcgct ccgtcaccgc ccggctgccg cacccaccct cgcacatcgt gagcacttgt
     9481 cgcatcattc gaaacgttgc tcgagcctgt cgaaccgatc ggcgcgcctg cgtccgaggt
     9541 gcgaacggga tcccagacgg accgcgcgtc ccacttccgc cgtgcgcctg ctggcacaag
     9601 gttggacaat gctcaacaga tcccctgcct ccgcgacgcc cacccgacac agcaggagcg
     9661 acgatgactg actcgaccga tcacggccgc gagccgtggg tgaccgacat cgaggcggcg
     9721 acgctcgaca acaccgactt ccgctcgacg ctctggaccg gcgagcacct ccagctcacc
     9781 gtcatgtcca tcccggtcgg tggagacatc gggctcgagg tgcacccgga caacgaccag
     9841 ttcctgcgga tcgagcaagg caggggtcgc gtccagatgg gcaccgagaa ggacgacctg
     9901 gcattcgacc gagaggtcga ggacgactgg gtcatcctcg tgccggccgg gcactggcac
     9961 aacgtcacca acatcggtga cgagccgctg aaggtgtacg ccctgtacgg accgcccgac
    10021 cacaagcccg ggacggtcca ccacacccag caggacgcgc tgaacgaccc cgacgagcac
    10081 tgacccggct cgccgggccg gagggcggcg cacgctccgg gatcgcctcc aacgtcatga
    10141 gttcgtgaac gcgacggcag gatcgacccg gacgacaccg cgaacactca gcatgctcga
    10201 ggccaccccg gccaccagtg ccagcccgac cagccgtagg cagtccgcac cggtcaggct
    10261 gatgctcacg gggaacagcg ggatcagcgc ataggcaagc accagagcga caccaccggc
    10321 gaccaggctg atgatcgcgc cttcggacat catggacgtc agcagtcggt gcgtgctcac
    10381 gccgtacgcc ttgtacaccg cgaagtcccg atgccgatcg agcccggaga ggaacgacag
    10441 catgccggcg atgccggcgg cgaccagcca caacagccct gccacgatcc cgatcgcggt
    10501 cgtcgccacc gagagcggcc gtcgaaggtc gtcgcgcacc tgggtcggtg tcatcgcggc
    10561 cagcccgtcg ggcaacgcct ccggatttcc ccgcacgacg agagcacttg ccagccggcg
    10621 gccgtcgaag gcgatggact gcccgtcgcg caacgtgatg agcacggtgg gcgtgccgcc
    10681 gttgtaggtg aggccacgga cttgcccgac caccgtcagc cgccggccgg cgatctcgag
    10741 cgtgtcgccc acctccacac cgaggtgcac gtcgaccgcg gcctcgccga cgcgcgacgg
    10801 tgcccgtccg gaggtcgtcg cgggcgcgac aacaccgtcc ggctggtatg cgatgacgtt
    10861 gacgtcggtg aacccgttgc ccaccccgtc gaccacgtgc ctgaagatcg ccacaggtgt
    10921 gacttcttct gttccgggtt ccgcccgcac cgcatcgaca gccgtctgtc tgagcggaga
    10981 gttggcggtg aacggtccgg agacatcggt gggcacgatc caggtgtccg cgtcgaagaa
    11041 ggtgatcgtc cggtcggtct cccggaggaa cccgtcgtgc agcgccccga ggagcgcggt
    11101 catcccgagc acgagggaga tcgctgcgag acccagcacg aggcgcctgg cgcgccactg
    11161 catgtcacgg aacgtgatca gcatcaagag gacgcacttg cggcgacggc tcgggcccgg
    11221 atgtgcgtcg tcaccggcac cacgagcgtg atggtcagga gcgccaacgt ggctggaacg
    11281 cggacgtcga gcacgccgat gatccccatg accccgagcg cccagcccgc cagacatacg
    11341 gcaagcacgc gagccgcggc cgggtacgcc agcggactga ccatcaagaa cgcgagtgca
    11401 gccagtccga caccagcgag ggctggcggc ggcgacagaa agccgaggca gacggcggcc
    11461 acgcacgccg atgtcgtcgg cagtccgacg aagtcggaac gctcacggta atgcgccatg
    11521 aagtcggcca accggacgag cgcggccgcg acgtacgcca gcgctccagc ggcgacgacg
    11581 agctgctcgg cggagctgct gcccgacgtc acgtacgccg tgacggccac caccggcgcc
    11641 acaccgaagg tgaccccgtc tgcgaggcag tccaacggac cgcccaacgg cgttcctccc
    11701 catcgccggg ctgccgcgcc gtcgagtacg tcgaaaacgg tcccgcacac gaacagcagt
    11761 gcagccacct cataccgctg acccacgtcg agcgccgcga ccggttcgtc ccagcagatc
    11821 accaggatcg cggcagcccc gcacaacgcg ttgcacagtg tcagcacgtc ggcggcgccc
    11881 ggccggcgtg tcgaccaggt gctcacgtga gtccgctcgg ttgcgtcatc acgtccggcg
    11941 tggcgatgac ggttgcgccg ccgtacatcc ggtcgccttc gccggctgcg gcggagacac
    12001 ccaccggcaa cacgacgtcc acgcgcgatc cgaaccggat gaggccgatt gcctcgccac
    12061 gacgcagcac gtctccagtg cgtttgtggg tgacgatgcg gcgcgcgacg gtcccggcgt
    12121 actggatgag cacgatgtcg ccgtgcgccg tcgccagtgt ccactccatg cgttcgttgc
    12181 ggtgagcaga agggctgaac gccggccggt gtctccctcg gcggtaggtc tgctgcacga
    12241 ccgtggcgtc gcacggggcc cgcgtcacgt ggacgtcgag caggttgagg tacgtcgaga
    12301 tggtctcccg tcctcgctcg tccgtccgga cccactggac gacgccgtcc gcggcggcca
    12361 gcaggccctc ccccttcggg gtgcgccgcg ggtcacggaa gaaccaggtg caacctgcgg
    12421 cggagagcaa gaccaccaaa cgagcccacc gtgacgccac gacgagcgga gcgaaccaca
    12481 gcagtgccac gaaaccgggt gtcccccgtg cgagccggac ggtcatcgcg cctcgatcgt
    12541 gccgacggcg ctgtgttggc ccaacgacac aacgcggtcc gccagtggct cgaaccgcgc
    12601 atcatgagtg gcgacgacga ccagccgccc ttgcccgcgc agaccgcgga tgagacggag
    12661 gatggcgtcg acctgcacgg gatcgagatg tgcggttggc tcgtcggcca ggatcaccgg
    12721 agcgttgttc accagtgccc gtgcgaatgc gacgcgctgc tgctggccac ccgacagcgt
    12781 gccagggcgc tggtgcgcat ggtcggcgag gtccacctca tccagcagtc gcctggcggt
    12841 acgacgagcg accgccgacc gtacgccggt gagaacgagc ggtgccatga cgttctcgag
    12901 agcgctgagg ctgggcacga gattgaacgt ctggaagacg atgccgacgt tgtttcggcg
    12961 ataggcgagc aggtcccgcc cctcgagatc gatcacgtcc cggccgtcca ggatcacccg
    13021 accggacgcc ggtctgagca gaccggccag aacggagagc aaggtggtct tgccgctgcc
    13081 actgggcccg agcgccagaa cgatctcgtc tcggcccgcc tcgaaggaga cgttctcgag
    13141 tacccgcgag cgatgcgagc cgagggtgta ctcgacggtg agcccgtcga cactcaggta
    13201 tggcgagacg tgctcaacct caccccgcat cgctcggcca tctccacggc tgaggcatcc
    13261 gcttccggtc agacagaggg gccacctcct ctatcggaca gggtccccca acgtgtggaa
    13321 ccatagcgtc gccattccag gcaccgcagt cgttcggcag aactcggagt cagatgacgc
    13381 agacgcttcg tagagccctc cttcggctcg tcatgcgcgt tcgacgaccg agcacgtccc
    13441 gtctgctgac cgggctaccg tcctccgtgc tggtcgcgct ccgtcgcgac ggcctggatc
    13501 cggaactggc cggcgccgcc gatcccatca ccaaagtgat acttccgctc ggcattcggg
    13561 gctggctcgt caccgggtat gacgaggtcc gtcaagtcct ggccgaccac gactccttca
    13621 gcaacgactt cgggaacctg gtcggccggg ccggtgtggc cgccggactc gacccgggcg
    13681 gcctcggctt caccgatccg ccgtaccaca cacggatgcg gcacatgctc acgcggcact
    13741 tcaccacccg ccggcttgcg gcacttgcgc cgcgggtctc ggcgatcgtc gagtcgagcc
    13801 tcgccgatgt cgagcgtgag ctcgatgcgt tcggccggac agacctcgtc gagcggtttg
    13861 cgattccggt accgacgctg accatctgtg aacttctcgg cgttccgtac gccgaccgtg
    13921 agcacttcca acaattgagc agcgcccggt tcgacctcac cggaggcgcc tccgcgtctc
    13981 tggatgcagt caacgagtcg ttgacctatc tcgaggacct ggtcgctcgc cgccgccggg
    14041 aacccgacga cggtctgctc gccggtctcg tcgccgacca tgggtcgcag ttcagcgacc
    14101 gcgagctcgc gggtgtcgcc gacgggctcc tgaccggagg tatcgagacc accgtcagca
    14161 ccttgggtct cggcagcctg gtcctcttgc aagacccggt cagctttgcg cggctcgcgc
    14221 aggacgaagt cttcgcggcg gcgtacgtcg aggagctcct gcgctacctc accgtcgtcc
    14281 aggtggcctt tccgcggttc gcgcggcgcg atgtcgacgt cggtgcctca ctggtgcgcc
    14341 gccacgatgt cgttctctgt tcgctgagcg cagccgggcg tgacccgcgc accggtgagc
    14401 atctgaacga ggtcgatccg gaagcggcac gtcgcccgca tctggctttc ggacacggca
    14461 tccaccgttg tgtcggagcg gagctggcgc gactggagct gcggctcgcg tttcccgcgc
    14521 tgtcgcgacg ccttcccgac ctgcggctgg cggtggacac gtccgagttg gagtatcgcg
    14581 cactgtcgat cgtcttcggg ctgccatcgc tgcccgtgac gtgctcacga tgacgagagg
    14641 acgcgaatct gtgggacgcc gttaacctgc cgatgtggac atcagcctct gtctggacac
    14701 ggcccggccc tggtcagagc tgctcgaact cgcccgccgt gccgacgacg aagcgctgta
    14761 cgcgatctac gtgcccgacc acttcggaag gcaccgggag gcgtggacac tgctgagcgc
    14821 actggccgcc tccacgcatc gagtgcgcct cggcgtgctg gtcctcggcg tgacacatcg
    14881 gcacgtgggt ctgctcggcg aaatggcgcg aaccctcggc gaagtcagca acgggaggct
    14941 ggtgctggga ctgggagccg catgggacga aggcgaacac cgatcgctcg ggcttgcgtt
    15001 tccggggcca ggtgaacgcc tcgacctcct ggaggcggcc ctcggacagc tggtagacgt
    15061 cggaccgcca cttctggtgg gaggtcgcgg cgagcgtcga acgatgccga tcgcggcgcg
    15121 gtacgcctcg atctggcaca gctgggccgc gccagacgag ttccaacgca agtgcgggat
    15181 catggaccgg ctctgccgcg acatcggacg agagccgtcg gccgtgcgtc gtgccacggg
    15241 cggagtactc ctcgacgagt acgactcggc agggtcgctg gcggcctatc ggcgtgccgg
    15301 tgtcgacgag ttcatcgttc gggaccaccg cgacgcaagc gtcactgact cgcttgccgc
    15361 cttgcccaac cagaggtagc cgttcacgcg caaggtgtgc agtgcacgtc gcgcaagccc
    15421 actgaacagc gcagtgccgc ctcaccctgg tgttccaact atttcgcacg cgctgaactc
    15481 ttgtgcggca tgctcgaatc catggggacg aacgactcgg agacggacct cgggatcacc
    15541 ctgctggagc cggcgcagag tcgggacgag cacctggtcg tgcgattgac cagcctcatc
    15601 aaccgggtgt acgagacagc ggagagcgga ctctggcatg acagcgccgc ccggaccacg
    15661 aaatcagagc tcgccgcgct gatcgaggag cgacagatcg ctgtggccac ccgcgagggc
    15721 gagatcgtgg gcagcgtgca gatccacgac gtttccaccg acatcagcga gttcgggatg
    15781 ctggtcgccg accccgacca tcgcgacacc ggagtcgggc gggcgcttgt cgactacgca
    15841 gagggtcgga gccgcgagag aggtctccgg gcgatccagc tcgagctgct tgtcccccgc
    15901 acctggcggc atccgagcaa ggagttcctc aaggagtggt acggacgcag gggctaccgg
    15961 ctcgtcggga caacaacgat cgaacatact catccgcacc tggccccgct gctcgccacg
    16021 ccctgcgacc tcaccatcta cgagaagcct ttgcaggcga ccgacgtcgg ctgatcgcgg
    16081 tgatctgacg ttccgcggcc gctatccgaa gatcgcaccg caggcctgta tgcccatcgc
    16141 ctggaactca ttggcggccg ccatgatgtt gacgcgccag tcgtcgggtc ccagcagatt
    16201 gagcatctcg actgcctgtt gcaagtcctg tagcgcccga acccacgcat ctgggttgtt
    16261 cggcggcgaa cccagctgcg ccaacgcgtt gaccaggtac tgcgtgtcat g
//
LOCUS       DATBFZ010000431         5335 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_3254181, whole genome shotgun sequence.
ACCESSION   DATBFZ010000431 DATBFZ010000000
VERSION     DATBFZ010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 5335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5335
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3254181"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            3..158
                     /locus_tag="VH419_13095"
     CDS             3..158
                     /locus_tag="VH419_13095"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044599.1"
                     /translation="MVTAWTDEPQGRVVMGVEHRDRPLHGVQFHPESVLTEHGAVLVE
                     NFLRMRR"
     gene            155..1507
                     /locus_tag="VH419_13100"
     CDS             155..1507
                     /locus_tag="VH419_13100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008356110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HEY7044600.1"
                     /translation="MTVEPLPWVEPEDFVVAHVAGRDRAFWLDGAGSRPWSGRRSFVG
                     WLRPDDLSLTYDARTTTVMACRAGSAHPVGSDIFAAVQQLTPVVAHPGAGWTGYFGYA
                     ARPDLPARLDDGGAFRDGAAPDSCWMRADRMVAFDHEHHTVTAVGDDAWRAEVADLLC
                     RTATAHPATARPEAADVQTLDRGRYAEAFVRVQSELRRGNSYEANLTYRSQVVSSLDA
                     LSAYRRLRRINPAPYAAYFTHHGVSLLSSSPERFARIEPDGWLETRPIKGTTPRHEDA
                     DADVEAAHRLRRDPKFRGENLMIVDLLRNDLSIVCDVGTVEVADLMHVESYATVHQLV
                     TTIRGRLRGDVRPVQAISALFPGGSMTGAPKLRTMQIIAEVETTPRGAYSGALGWIGD
                     DGRADLGIVIRSLVHRDGIYTLGTGGGITVRSDCDEEYAESQWKLSAMLAALRPDSAG
                     "
     gene            complement(1688..2869)
                     /locus_tag="VH419_13105"
     CDS             complement(1688..2869)
                     /locus_tag="VH419_13105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008361075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEY7044601.1"
                     /translation="MTNDTFPPSYPPGPSLTPLAAKSRRGGRVVGAALLVGALAGGTA
                     GFGAAAVMDNDGAASMAASPTALAVAPSADETAANATLSGTESAAAKALPSVVKIYAS
                     GPQASGSGSGIILTKDGEILTNNHVVELAAGGGKLAVSFNDGSTAKATILGRDPLTDL
                     AVIKADGQQNLTPATLGNSGSLEVGQQVVAVGAPFGLESTVTTGIVSALDRPVSTQGE
                     SPSDPETIFPAIQTDAAINPGNSGGALIDLAGNVVGINSAIKTDTSGEGGSIGLGFAI
                     PIDDALPIVQELRAGDTPTHARIGVSVGNATDSIGLPDGALVGTIDPGTAAAEAGLEK
                     GDVITKIDDQLISDADSLVATIRSYRPGDKVTLTIAKSDHGSVTSDTHTIDLTLGSDE
                     S"
     gene            complement(3357..4844)
                     /locus_tag="VH419_13110"
     CDS             complement(3357..4844)
                     /locus_tag="VH419_13110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012924426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY7044602.1"
                     /translation="MTTPASFGAPQPERTHRMSLAARISLLAACAVGLAVAAAALAAY
                     VTLRSQLYDRQDAALLDRAHAVVNSGVITEVTLSRLPIDFLTAADLRVYLLYADGRIQ
                     PADHEYLDPPSTGESLVAAGRLDHALRTVDDDGDEFRVAAVNAGPGVALMLVQSTEPI
                     QSTLDRLGLVLLLVGAGGIVAAAAIGLAVARSSLMPVRRLSAAADHIAQTEELRPIEV
                     TGDDELASLAASFNTMLAALAASRERQRRLVADAGHELRTPLTSLRTNLELLAQADRR
                     GGMTTAQRDEIFLDATAQVEELSTLVGDLVELAREEPVALTPERIDFEGVVARAVDRV
                     RRRAPDVTFDVDTASWWVFGEATILERAVTNLLDNAAKYGPPDGTVTVRLADGVLTVA
                     DEGPGIGSADMPMVFERFYRSEAARALPGSGLGLSIVEQAVARHGGQVHIANHAPHGA
                     VFTLWIPGQSTELLPTDFDIGGTSSATPPRPAVPTVSDEITAPRP"
     gene            complement(4841..>5335)
                     /locus_tag="VH419_13115"
     CDS             complement(4841..>5335)
                     /locus_tag="VH419_13115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009083592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY7044603.1"
                     /translation="RAAGNDLPILVLTARDSVNDRVDGLDAGADDYLAKPFALDELLA
                     RVRAMLRRRTLGTSQTEDEGPLVYADLTMNPVTRDVERAGRPITLTRTEWDLLELFMR
                     RPKRVLERAFILEEVWGYDFPTTANSLEVYVGYVRRKTEAGGEPRLLHTVRGVGYVLR
                     ETPP"
BASE COUNT          815 a         1771 c         1892 g          857 t
ORIGIN      
        1 tgatggtcac tgcgtggacc gacgaaccgc aaggccgcgt cgtcatgggc gtggaacacc
       61 gcgatcggcc cttgcatggc gtccagttcc atccagagtc ggtgctcacc gagcacggcg
      121 ctgtgctcgt ggagaacttc ctgcggatgc gtcgatgacc gtcgagccgt tgccctgggt
      181 cgaaccggag gacttcgtcg ttgcccatgt cgccggacgg gaccgagcgt tctggctgga
      241 cggtgcaggc tctcgtcctt ggtcggggcg acggtcgttc gtgggttggc tccggccgga
      301 cgacctcagc ctgacgtacg acgcacgaac cacgacggtg atggcgtgtc gagccggctc
      361 cgcccacccc gtggggtcgg acatcttcgc cgcggtgcaa cagctgacgc cggtcgtcgc
      421 gcatcccggt gccggttgga ccggctactt cgggtacgcc gcgcgtcccg acctgccggc
      481 ccgactcgac gacggcggtg ccttccgtga cggcgcggcc cccgactcgt gctggatgcg
      541 tgcggaccgg atggtcgcgt tcgaccacga gcaccacacc gtcaccgcgg tcggggacga
      601 tgcgtggcgg gcggaggtcg ctgacctgct gtgccggacc gccaccgcgc acccggcgac
      661 agcccgcccg gaggccgccg acgtccagac cttggaccgt ggtcgctatg cggaggcgtt
      721 cgtccgggtc cagtccgaac tgcgacgggg caactcctac gaggccaacc tgacgtaccg
      781 aagccaggtg gtgagcagtc tggacgcgct gtcggcgtac cggcgactgc gacgcatcaa
      841 ccctgctccg tacgcggcgt acttcaccca tcacggcgtc agcctgctca gcagctcacc
      901 ggagcggttt gccaggatcg aaccggacgg ttggctggag acacgaccga tcaagggcac
      961 gacaccacgt cacgaagacg ccgacgccga cgtcgaggcg gctcatcgac tacgacgcga
     1021 cccgaagttc cgtggcgaga acctgatgat cgtcgacctg ttgcgcaacg acctgtcgat
     1081 cgtctgcgac gtgggaacgg tcgaagtcgc cgatctcatg catgtggagt cctatgcgac
     1141 ggtgcaccag ctcgtcacca cgatcagggg ccgactacgt ggcgacgtgc gaccggtcca
     1201 ggccatcagc gcgctcttcc ccggcggctc gatgacgggg gcgccgaaac tgcgcacgat
     1261 gcagatcatc gccgaggtcg agaccacgcc acgtggtgcg tactccggtg cactgggctg
     1321 gatcggcgac gacggccgcg ccgacctcgg gatcgtcatc cgcagtctcg ttcatcgcga
     1381 cgggatctac acgctcggga ccggcggcgg tatcacggtg cgttcggact gcgacgagga
     1441 gtacgccgag tcgcagtgga agctcagcgc catgctcgcc gcgctccgtc cggactccgc
     1501 tggttgactc ggtggcggcg ctccgccggc cgagcctgat cggtccgcgg ttcgcgggaa
     1561 aacggctcct gtcgcagacc tctcgtggac cgttttcccg caaacggcgg gaagggaagg
     1621 accggacacc ccagccagtt gatgtccggt ccctcgatcg tgggtggcgc gcgttgtcag
     1681 cggcgtgtca ggactcgtcc gatccgagtg tcaggtcgat cgtgtgggtg tcgctggtca
     1741 ccgatccgtg gtcggacttc gcaatggtga gcgtcacctt gtcgcccggc cggtaggacc
     1801 ggatggtggc gaccagggag tcggcgtcac tgatcagctg gtcgtcgatc ttcgtgatga
     1861 cgtcgccctt ctcgaggccg gcctccgcgg ccgccgtacc agggtcgatg gtgccgacga
     1921 gggcgccgtc cggcagaccg atcgagtcgg tggcgttgcc cacggagaca ccgatccggg
     1981 catgggtcgg ggtgtcaccc gcgcgcagct cctggacgat cgggagggca tcgtcgatcg
     2041 ggatcgcgaa gccgagtccg atcgaaccgc cctcgccact ggtgtcggtc ttgatcgcgg
     2101 agttgattcc gacgacgttg ccggcgaggt cgatgagcgc ccctccggag ttgcctgggt
     2161 tgatggcggc gtcggtctgg atcgccggga agatcgtctc cgggtcgctc ggcgactcgc
     2221 cctgggtgct caccgggcgg tcgagcgccg acacgatacc ggtcgtgacg gtgctctcga
     2281 gaccgaacgg cgcgccgacc gcgacgacct gctgtccgac ttcgagggag ccggagttgc
     2341 cgagcgtggc gggggtgagg ttctgctgcc cgtcggcctt gatgacggcg aggtcggtga
     2401 gcgggtcacg gcccaggatc gtggccttgg cggtgctgcc gtcgttgaag ctgactgcca
     2461 gcttgccgcc gccggcggcg agctcgacca cgtggttgtt ggtcaggatc tcgccgtcct
     2521 tcgtcaagat gatgccggat ccggagccgc tggcctgtgg tccgctggcg tagatcttga
     2581 cgaccgacgg aagcgccttt gccgccgccg actccgtgcc gctgagcgtg gcgttggcgg
     2641 cggtttcgtc agctgacgga gccacggcca acgccgtggg tgacgcggcc atcgaggcgg
     2701 cgccgtcgtt gtccatcaca gcggctgcac cgaaacctgc cgtgccgccg gccaaggcac
     2761 ccacgagcag ggctgcccca acaacacgtc ccccccgacg cgacttcgct gcaaggggag
     2821 tcagcgatgg gccgggtggg tacgaagggg ggaacgtgtc gttggtcatg gctccactct
     2881 gaggtgccac gctgaaagcc cgctgagatc gcgccgagag gtttctgtga ctccggggct
     2941 gcggttgccg cgtcggcgcg gccgcttgcc gaatagccgc ctacagcacc aatcggaaag
     3001 tcctccttgt gggtttgcga tgtttacagc ccaagcggcg tgtgcacatc gcaaacctga
     3061 ggtggacgga gtgggtttgg gatgtggaca tcgcaaaccg cgggcgctca gcgccaaatg
     3121 tccgaccacc gccagtcaca caccggacaa ggaacggtga gccggccggc ctgttcagtg
     3181 ggcacgaaca ttccccgcgc ttcgctgcca tccaccccag ccaccgaagc agggcagccg
     3241 ggtgagcacg accgccactc gcgaccatgt cccgcacacg ggggcccggc gggaacccag
     3301 gccagctggg aagtcacggg catcgcgggc gtttcgaacg actcacggcg ggtcggtcac
     3361 gggcgaggag cggtgatctc gtcggagacg gttgggacag caggtcgagg aggcgtcgcc
     3421 gagctggtcc cgccgatgtc gaagtctgtg ggtagcagtt cggttgactg gccgggaatc
     3481 cagagcgtga agaccgcgcc gtgtggtgcg tggttggcga tgtgcacctg gccgccgtgc
     3541 cgcgcgaccg cctgttccac gatcgacaga ccgagcccgg accccggcaa cgcacgggcc
     3601 gcctccgacc ggtagaaccg ttcgaagacc atcggcatgt cggcggaccc gatgcccggg
     3661 ccttcgtccg cgacggtgag cacgccatcg gcgagtcgga ccgtgaccgt gccgtccggt
     3721 gggccgtact tcgccgcgtt gtcgagcaga ttggtgacgg ctcgttcgag gatggtcgcc
     3781 tcaccgaaca cccaccacga cgcggtgtcg acgtcgaacg tcacatcagg tgcccgccga
     3841 cgtacgcgat ccacggcacg ggcgacgacg ccttcgaagt cgatccgttc cggcgtgagt
     3901 gccaccggct cctcgcgggc gagctcgacg aggtccccga ccaacgtgga gagctcttcg
     3961 acctgggcag tcgcgtcgag gaagatctcg tcacgttgcg ccgtcgtcat gccgccgcga
     4021 cggtcggcct gcgccagcag ctcgaggttc gttcgcaggc tcgtgagcgg cgtccgcagc
     4081 tcgtgcccgg catccgcgac cagccggcgc tgtcgttcgc gggacgcggc caatgccgcc
     4141 agcatcgtgt tgaacgacgc cgccaggctg gccaactcgt cgtcaccggt gacctcgatc
     4201 ggtcgcagct cctccgtctg tgcgatgtgg tcggcggccg cgctcagccg gcgtacgggc
     4261 atgagcgatg agcgggcgac tgcaaggccg atcgccgccg cagcgacgat tccccccgcg
     4321 ccgaccagca gcaggaccag gccgagccgg tcgagtgtgc tctggatggg ttctgtcgac
     4381 tggacgagca tgagcgccac gcctggtccg gcgttgactg ccgccacccg gaactcgtcc
     4441 ccgtcgtcgt cgacagtccg cagggcgtgg tcgagcctgc cggcagccac gagcgactcg
     4501 ccggtcgagg ggggatccag gtactcgtgg tcggcgggtt ggatacgccc gtcggcgtag
     4561 agcaggtaga cgcgaaggtc ggccgcggtc aggaagtcga tcggaagcct cgacagcgtg
     4621 acctcggtga tgaccccgct gttgacgacg gcgtgggccc ggtcgagcag cgccgcgtcc
     4681 tggcggtcgt agagctgtga acgcagcgtg acgtacgccg ccagggcggc tgcggcgacc
     4741 gcgagcccga cggcgcaggc ggccagcagc gagatacgcg ccgccagcga catccggtgc
     4801 gtccgctcgg gctggggcgc accgaacgat gccggggtcg tcacggaggc gtctcgcgca
     4861 agacgtaccc cacgcctcgc acggtgtgca acagccgagg ctcaccaccg gcctcggtct
     4921 tgcgccgcac gtatccgacg tacacctcga gcgagttcgc ggtcgtcggg aagtcgtaac
     4981 cccagacctc ttcgaggatg aacgcacgct cgaggacacg cttgggtcga cgcatgaaga
     5041 gctcgagcag gtcccactcc gtccgggtga gcgtgatcgg gcgtccggcc cgctcgacgt
     5101 ccctggtgac ggggttcatc gtgaggtcgg cgtacaccag cggaccctcg tcctcggtct
     5161 gactcgttcc gagggtccgg cggcgcagca tcgcccggac gcgcgcaagc agctcgtcga
     5221 gcgcgaacgg cttcgcgagg tagtcgtcgg cgcccgcatc caggccatcc acgcgatcgt
     5281 tgaccgagtc acgtgcggtc agcaccagga tggggaggtc gttgccggcg gcgcg
//
LOCUS       DATBFZ010000432         9141 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_4746510, whole genome shotgun sequence.
ACCESSION   DATBFZ010000432 DATBFZ010000000
VERSION     DATBFZ010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 9141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9141
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4746510"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(276..986)
                     /locus_tag="VH419_13120"
     CDS             complement(276..986)
                     /locus_tag="VH419_13120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044604.1"
                     /translation="MSTFSLDRSAAATSGPADGQAHRTLTRVSARFGVAFAICQIGVM
                     VLMATLVLPHGGSPSDPPLQRGQHVLDAETLYQVGNYVFMIAGSLLLGFIGVVQVRMR
                     RVDPSGVLATVAVAAGTLLAVVWPMAGMLHDVALETASSGTDLRILAGWDSVAPFALA
                     FSVLPRLFFIGALVLGLRLAGTAPWLQRIGVVLLPVSLIGSATLVSGALFPVLALSTL
                     GFELWVGALAWHWLRVAE"
     gene            1161..2273
                     /locus_tag="VH419_13125"
     CDS             1161..2273
                     /locus_tag="VH419_13125"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEY7044605.1"
                     /translation="MGLLDRLRTTPVDAALTVAMLLAGLIQVVLFPIAGPGLGELFVV
                     GSTLPLAWRRTYPLESALVASAFWLMPLDGFPVLGFLVVILQFFAVGTRGEPRLAVAL
                     VTAWASATAIVGTLVGPESPVAVIGAVLVVVAPVLAGRVVRHHYRQNQALAALADQLR
                     EERRKAEDAAVGAERARIARELHDVVGHEVTLIAIQAEAAAVALRVTPERACEPVEAI
                     RSTAHRTLAEMRAVLDVLEPGGEVDRSSDGLAALADRARAAGIANSLSVTGTPPPSHT
                     PVSLAVNRIVRECLTNAGRHAPGEAVSLDVEWRTGEVVVHAMNRTDSPGGLAPGRGLT
                     GIRHRAELFGGTFTATDSGGVFDVRVHIPAVTGDLA"
     gene            2270..2926
                     /locus_tag="VH419_13130"
     CDS             2270..2926
                     /locus_tag="VH419_13130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008356944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY7044606.1"
                     /translation="MTRVLVVDDQDLVRHGLRMILELGGIEVVGEAADGAAAVTAATE
                     LEPDVVLMDLRMPGMDGIEAIRHIAAQGRSRVLALTTFDVDQHVVDAVRAGAVGFLLK
                     DVTADGLVDAVRRAAAGEPVVAPAVLTRMMDHFSSRPPVPPRQLPGLSDLSDREREIL
                     ALIGAGRSNSEIAEELVISMATVKTHVRHVFAKLDLRDRAQAVVAAHDAGLTLPRRDT
                     "
     gene            complement(2994..3569)
                     /locus_tag="VH419_13135"
     CDS             complement(2994..3569)
                     /locus_tag="VH419_13135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020578202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEY7044607.1"
                     /translation="MTVTYTFDVFSSLDGYGAASANWSGYWGKQGPELLDHRLALYSS
                     EQRMVFGANTYRAFARMLASSSEDSDVRDPWVTRMRSLPATVVSTTLEEPLDWPNATV
                     VSGDAVDVVARLKEESDVPLRSHGSLSLNRSLMAAGLVDHVQVTLFPVITGQTGLEPI
                     FRSAADFDLELVEHRTLDGHIQELIYRPTLH"
     gene            3863..4705
                     /locus_tag="VH419_13140"
     CDS             3863..4705
                     /locus_tag="VH419_13140"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY7044608.1"
                     /translation="MVTSPREDRDVFRLAPSMDEAVLRTLAERLEFRATDAGYVALSQ
                     AYFERLPLSPPCRVLALGCGTGVEVRALRRMAAPEVSIVGVDHSDALVERARQETAAA
                     GLSENVEYVTGDAHDLAYSDAEFDVVTLHTLISHVEDPSRVLSEARRVLSPGGMAAVF
                     DGDYASLTFSHPDPVLAITIEERLKQLMVANPRVMRDLARLLQEADLELVAADGEVYA
                     DIGTGSFWANAAEAYGVLLERSGLLPVEVIQGWRAEQADAVARRAFFGASVYYTYLAR
                     RPTS"
     gene            4897..5538
                     /locus_tag="VH419_13145"
     CDS             4897..5538
                     /locus_tag="VH419_13145"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY7044609.1"
                     /translation="MADPEPSFARNAAAAAYYEDGAAEYDEWYLGIGQYAERDRPECH
                     ADVSQLVGLVATLPPARTLDVACGSGFLSRHVRGAVVGLDQSPSMARLAQSRIPGSMV
                     VRGDALCLPFDDRSFDRVLTGHFYGHLASEEREAFLSEASRVAAELVVVDSALRPELV
                     PELWEDRLLNDGSRHRIYKRYFTADQLAHEIEGEVVMAGKWFVAARAPLPAHN"
     gene            complement(5630..8893)
                     /locus_tag="VH419_13150"
     CDS             complement(5630..8893)
                     /locus_tag="VH419_13150"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044610.1"
                     /translation="MDTTGTTDPNPDNDSASFASTASVSADVSVTKAAPATALAGGTV
                     VYNLTVSNAGPSAADSTTVTDPLPIGLTVVSATTDQGSCTPASSVSCDLGTVNPGSTV
                     HITITARVDPGVASASVIANEAAVSSTTPDPTESNNSDRETTTIETAADIRVTKELLT
                     QPPLASGQFLQFRMLVRNDGPSDAATVSMRDSIGSLSAGVVDITIDGTQGFDQCEIDP
                     SGDLFCQLGDLPVGETSEVLLTVIPDPGASLNTRFANDVTVAAVTSDPDHDNNAAEAP
                     FQFTRTEADLDIAKDGAAQLVAGTEFSYTITVDNTTGPSNAADVVVTDTLPAGLTATQ
                     ALPSQGSCTIAGTVVTCSLGTVLRILQTPVITITGTVDPDAATGVVTNTASVTTSTPD
                     PEQSNNTATFDSTIGTQANLSLTKTADSDPLVAGTEVGYTLTVTNAGPSDASPVTLVD
                     VLPTAVAFDPTKSDPSCVQNATVDCALGNVPAGATRVVRISGQLDPAYAGATLTNTAS
                     IDSPVDDPDTSDNTATDVSDVQQQADLSVSKIAEATETPAAGSDLTYDISVVNHGPSD
                     AVNASFTDILPLPPTSFILPSQGDVTCALDGLTVTCTAPRLPAGESRTGQITIHIPAD
                     HTAGPMDNPASVTSDTSDPVQANNDVVRQVNVVLVADLKMTKTLLTDPIVAGEPVTYR
                     LTVENQGPSTAPNVLVSDPLANNVTLTGAPPGCDATQGEEHQTILACRLTTLAVGESA
                     SATITVTPSPDATGDLTNAATAGADSLDPAPDDNTSTVTAPITSEVDLAVRITGPAKV
                     QAGGTADFHIGYSNRGPSNATDVVITDTLPAGLTVPNPPAGCTISGRTLTCNVGALVA
                     GASGSLDLTVAVDASLKGGARLIQTVTISADQTDVNPDNDTASISSTVGRATDVAVTV
                     TADGPTVMQGGVAGYSVTVTNNGPLPATAVVLKNVLPPEVTNPRDPPDCTFTGSTATC
                     SLGDLAVGASTTIPFRGDVPAGTPVGSKLVDNASVTHDETDSVAVNNQDKATISVVAQ
                     ASGPPPPTQPPPQGPPPSNGPLPSTGAELLSLILVALILIGAGAGLTLAARARRPSK"
     gene            8802..>9141
                     /locus_tag="VH419_13155"
     CDS             8802..>9141
                     /locus_tag="VH419_13155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044611.1"
                     /translation="MTDTSAETLAVLANEALSLSGFGSVVPVVSTVAVFVMAPPGNVA
                     ITVALTVKASDPPTARSGAVQVTVCPLTEQPGAVMSVSEVGSVSLTVTPRAREGPLLV
                     AVRVYEIGPPA"
BASE COUNT         1436 a         2881 c         3223 g         1601 t
ORIGIN      
        1 caggggtcgt agacgctcgt cagtcgccgc ctccatcgga cagggactcg accgcacagt
       61 cggcaagacc ggctcggcac gactgggctc ttcacttccc ggaaccggct gatgggcagc
      121 ctcgtcacgc acgcggaccc gggtccgtcc cctggttgag gaagtcttgt cgcgttgctg
      181 acctggccgc ttggtgagct cgacgaagtg tgccccgccc aggcagcgtc ggctgcctgg
      241 gcgggacggg gtgtgtccga tcatgcatgg accggtcact cggcgacgcg cagccagtgc
      301 caggccagag cgcctaccca cagttcgaag ccgagcgtgc tcagtgccag aacggggaag
      361 agcgcgccgc tgaccagggt ggcgctcccg atcaggctga cgggaaggag cacgacgccg
      421 atgcgctgca accatggtgc ggtccctgcg agccgcagac cgagcacgag cgcgccgatg
      481 aagaacagtc gtggcagtac ggagaacgcg agggcgaacg gtgcgaccga gtcccaaccg
      541 gccaagatgc gcaggtcggt gccggagctc gcggtttcca gtgcgacgtc gtgcagcatg
      601 ccggccatcg gccagacaac ggccaacagc gtccccgcgg cgacagcgac ggtggcgaga
      661 acacccgagg ggtcgacccg ccgcatgcgg acctggacga cgccaatgaa accgagtagc
      721 agagagccgg cgatcatgaa gacgtagttg ccgacctggt acaacgtctc cgcgtcgagg
      781 acatgttgtc cgcgctgcaa tggggggtcg ctgggagagc cgccgtgtgg aaggacaagc
      841 gtcgccatca gcaccatcac accgatctgg cagatggcga acgcgacgcc gaatcgagcg
      901 ctgactctgg tcagcgtccg gtgggcttga ccgtctgcgg gtccgctcgt cgcggcggcc
      961 gatcggtcga gactgaaagt ggacaatgga ctgcctttcg ttgtgtcacg gcccgctcga
     1021 gccggtgcca aggacaatag aaacccggca gtgcccgcca catcgaccta cgggcggttc
     1081 tacggtcagc ccggtacttc gacccgcgtc agcccagagg ttgatgccga aggggacgcg
     1141 gctcgcctac tctcacctgc gtgggattgc tcgacagact tcgtacgacg ccggtggacg
     1201 cggcactgac ggtcgcgatg ttgctggcgg ggctgattca ggtggtgttg ttccccatcg
     1261 caggtccggg acttggcgaa ctgttcgtcg tggggtcgac gctgccgctg gcttggcggc
     1321 gtacctatcc gctggagtcc gcactggtgg cgtccgcctt ctggctgatg cctctggacg
     1381 ggttcccggt gctgggcttc ctcgtcgtga tcctgcagtt cttcgcggtc ggaacccgcg
     1441 gcgaaccacg gctcgcagtg gctctcgtca ccgcgtgggc atcggcgacg gcgattgtcg
     1501 gaacccttgt cgggcccgag tctccagtag ccgtgatcgg tgccgtcctc gtggtggtag
     1561 cgccggtgct tgccggccga gtggttcgac atcactaccg gcagaaccag gcgctggcgg
     1621 cgctggccga tcagctccgc gaggaacgca ggaaggcgga ggatgcggcc gtgggtgccg
     1681 agcgggcccg catcgctcgt gagctgcacg acgtggtcgg tcacgaggtg acgctcatcg
     1741 cgattcaggc cgaagccgcc gcagtcgcgc tgcgcgtcac acccgagcgt gcctgcgaac
     1801 ccgtcgaggc gatccgctcc accgcccacc gaaccttggc ggagatgcgc gcagtgctgg
     1861 acgtgctcga accaggtggc gaagtcgacc gaagttcgga cgggctggcc gcactcgcag
     1921 accgtgcccg ggccgccgga atcgccaact cgctgtcggt gaccgggacg ccgccaccga
     1981 gccatacgcc ggtctcgctc gcggtcaacc ggatcgtgcg cgagtgcctc accaacgccg
     2041 gccgccacgc tcccggcgag gccgtctcac tcgacgtgga atggaggaca ggagaggtcg
     2101 tggtccatgc gatgaaccgc accgactcgc ctggaggctt ggctccggga cgaggcctga
     2161 ccggcatccg ccaccgtgcc gagttgttcg gtggcacgtt cacggctacc gacagcggtg
     2221 gcgtcttcga tgtccgagtc cacatcccag cggtcacagg cgacctcgca tgacacgggt
     2281 gctcgtggtc gacgaccaag acctcgtccg ccatgggctc cggatgatcc tcgaactcgg
     2341 cgggatcgag gtcgtcggcg aagcggccga tggcgcagca gccgtgaccg ctgcgactga
     2401 actggaaccc gacgtggtgc tgatggacct ccgcatgccc ggcatggacg ggatcgaggc
     2461 catccgacac atcgcagcac aaggacgctc ccgcgtcctc gcgctcacca cgttcgatgt
     2521 cgaccaacac gtcgtcgatg cagtgcgcgc cggagccgtc gggttcttgc tcaaggacgt
     2581 cacggcagac gggctggttg acgcagtacg ccgcgcggcg gccggcgagc ccgtcgtcgc
     2641 gcccgctgtc ctcacccgga tgatggacca cttctcgagc cgcccgcccg tgccgccacg
     2701 gcagctgcca ggactgtcgg acctcagcga ccgcgaacgc gagattctgg cgctgatcgg
     2761 cgccggacgc tcgaactccg aaatcgccga agaactggtc atcagtatgg ccaccgtcaa
     2821 gacccacgta cggcacgtct tcgccaagct cgacctgcgc gatcgcgctc aggccgtggt
     2881 ggcagcacac gacgccgggc tcacactccc aaggcgcgac acctagtgtc cctcgcccgg
     2941 cagcagcccc gccactgtca atggcccaac gactgtcaat ggcccaacga ctgtcaatgg
     3001 agggtgggcc ggtagatgag ctcttggatg tgaccgtcca gcgttcggtg ctcgaccagt
     3061 tccaggtcga agtcggccgc actccggaag atcggctcca gcccggtctg accggtgatg
     3121 accgggaaca gcgtcacctg tacgtggtcg accaggccgg cggccatcag cgaccggttc
     3181 aacgacaggc tgccgtgcga gcgtaacggc acgtcggact cctccttgag ccgggcgacg
     3241 acgtcgacgg cgtcaccgct cacgacggtc gcgttcggcc agtcgagagg ctcttccagg
     3301 gtcgtcgaaa ccaccgttgc cggcaggctc ctcatccggg tgacccatgg gtcccttacg
     3361 tcggagtcct cgctgctgga ggccagcatc cgcgcgaatg ctcgataggt gtttgccccg
     3421 aagaccatcc gctgctcgct gctgtacaag gcaaggcggt ggtcgagcaa ctcggggccc
     3481 tgcttacccc agtagccgct ccagttggcg ctggcggcgc cgtaaccgtc aaggctggaa
     3541 aagacgtcga aggtgtaggt gacggtcatg tcgctctcct cgaggcggtc ggatcagtgt
     3601 ggatacagac tgaggactac cgcgggactc atcgccgtcg ggtcggccat cacgttgtgc
     3661 gggcgatcga tcggtcacgt tcgatggcag tcaacgccgc ggtccaaggg ggccaccgac
     3721 ccggcatatc aacagtcgcg gcgggcggtc gtgatgaagc cgaggtccac ggtcggacgg
     3781 tcgtctcggg cggcgagcac ctcggggcgt aggctgactg ccgggcgctc gggttgccga
     3841 ggtagtgccg cgcggaggac tgatggtcac gagcccgcgg gaggatcggg atgtcttccg
     3901 cctcgcgccg agcatggacg aggctgtgct gcggacgctg gccgagcgac tggagttccg
     3961 tgccacggat gccggctacg ttgcattgtc acaggcgtac ttcgaacgcc tgccgttgtc
     4021 gcccccatgc cgggttcttg ccctcggctg cggcaccggc gtcgaagtcc gtgccttgcg
     4081 acggatggcc gcccctgagg tttccattgt aggtgtcgat cacagtgacg cactggtcga
     4141 gcgcgcccgt caggaaacgg cagccgccgg attgtcggaa aacgtcgagt acgtgacggg
     4201 ggatgcacac gatctggcct actccgacgc agagttcgat gtagtgacgc tccataccct
     4261 gatcagtcac gtggaagatc cgagcagggt gctctcggag gcgcgtcgcg tgctcagtcc
     4321 ggggggtatg gccgccgtct tcgacggtga ctatgcctcg ttgaccttct cccatccaga
     4381 ccccgtcctc gcgatcacaa tcgaggagag gctcaaacag ctcatggtgg cgaacccccg
     4441 ggtgatgcgg gacctcgcgc ggcttctcca ggaggccgat ctggaactcg ttgccgcgga
     4501 cggagaagtg tacgccgaca tcggcaccgg cagcttctgg gcgaacgcgg ccgaggcgta
     4561 tggggtgctg ctggagcgtt ccgggcttct cccggttgaa gtgattcagg gctggcgagc
     4621 cgagcaggcg gacgccgtcg cgcgccgggc attcttcgga gcctcggtct actacaccta
     4681 tctggcgcgc cgaccgacga gctaggcctc acgtggtcgg gcgcgcacac cgctcatcag
     4741 cgcttccacg cgcggagctg ctccccgttc gatcgagtca ttcagatcac tgcgcaccgt
     4801 tggtcgagcc acgggatcgc ggaatgaggg tgccgtcctg aacacggtgg agggttcgcc
     4861 ttttcaaccc gcctggaagc ccacacaatg gcggacatgg ccgatccaga gccgtccttt
     4921 gcacgcaacg ccgccgcggc ggcgtactac gaagacggtg ccgcagagta cgacgagtgg
     4981 tacctgggca tcggacagta cgccgaacgc gatcggcccg aatgtcacgc ggacgtgtcg
     5041 cagctcgtcg gcctggtcgc cacgctgccc cctgcccgga cgcttgacgt ggcctgcggc
     5101 agcggattcc tttccaggca cgtacgcggt gccgtcgtcg gcctcgacca gagtccttcg
     5161 atggcgcggc tggcccagtc ccggataccc gggagcatgg tggtgcgcgg tgatgcactg
     5221 tgtctgccct tcgatgatcg gtccttcgat cgggtgctga ccggacactt ctacgggcat
     5281 cttgcctccg aggagcggga ggcgttcctg tcggaagcgt cgcgggttgc ggccgagctc
     5341 gtcgtggtgg actccgcgct acggccggag ctggtgccag agctgtggga ggaccgtctg
     5401 ctcaacgacg gcagccgaca ccggatctac aagcggtact tcaccgccga ccagctcgcg
     5461 cacgagatcg agggcgaagt ggtgatggct ggaaaatggt tcgtggcagc ccgcgccccg
     5521 cttccggccc acaactaacg atccgcgaag ggcgagttct tcaggagcca tcaccacgca
     5581 ccaaagcccc gggtgcggcc gccccgaccg cggaagggcc ggtgaaggtt cacttgctgg
     5641 gccggcgggc gcgggcggcg agggtgaggc cggcaccggc gccgatcagg atcagtgcga
     5701 cgaggatgag gctgagcagc tccgcaccgg tcgacggcag cgggccgttg gacggtggag
     5761 gcccctgtgg cggaggttgc gtcggtggcg gcggaccgga agcctgtgca acgacactga
     5821 tcgtcgcctt gtcctggttg ttgaccgcga cggaatcggt ctcgtcatgg gtgaccgaag
     5881 cgttgtcgac gagcttcgag ccgaccggcg tacccgctgg cacgtcgccc cggaacggaa
     5941 tcgtcgtact cgcgccgacc gccaggtcgc cgagcgagca ggtggcggtg ctgccggtga
     6001 acgtgcagtc cggcgggtcg cgcgggttgg tcacctctgg cggcaagacg ttcttcagga
     6061 cgactgcggt tgcgggcagc ggaccgttgt tcgtgaccgt caccgagtag ccggcgactc
     6121 cgccctgcat gacggtcggc ccgtccgcgg tgacggtgac agccacgtcc gtggcccgac
     6181 cgaccgtgct cgagatcgac gccgtgtcgt tgtctgggtt gacgtcggtc tggtctgcgg
     6241 agatcgtcac cgtctggatc agccgtgcgc cccccttcag cgaggcatcg accgcgaccg
     6301 tgaggtcgag gctcccggat gcgccggcga ccagagcccc gacgttgcag gtgagcgtcc
     6361 tcccagagat ggtgcagccg gcgggtgggt tcgggacggt gagccccgcg ggcagcgtgt
     6421 cggtgatgac gacgtccgtg gcgttcgacg gacccctgtt gctgtaaccg atgtggaagt
     6481 ccgccgtccc gccggcctgg accttggccg gaccggtgat ccggactgcc agatcgacct
     6541 cgctcgtgat cggagcggtc acggtcgacg tgttgtcgtc gggtgcaggg tcgagcgagt
     6601 ccgcaccagc ggtggcggcg ttggtcagat cgccggtcgc atccggcgat ggcgtcaccg
     6661 tgatcgtcgc gctggccgac tcaccgacgg cgagcgtggt gagtcggcac gcgaggatcg
     6721 tctggtgctc ctcaccctgg gtcgcgtcac agccaggcgg tgccccggtg agcgtcacgt
     6781 tgttggccag cggatcgctc acgaggacgt tgggtgcggt agacggaccc tggttctcaa
     6841 cggtgaggcg atacgtgacg ggttcgccgg cgacgatcgg gtcggtgagc agcgtcttgg
     6901 tcatcttcag gtccgcgacg agtacgacgt tgacttgccg gacgacgtcg ttgttggcct
     6961 ggaccgggtc tgaggtgtcg gaggtgacag atgcggggtt gtccatcggg ccggcggtgt
     7021 ggtcggcagg gatgtggatc gtgatctgcc cggtgcggct ctcacctgcc ggcaggcgcg
     7081 gcgcggtgca ggtcacggtc aggccgtcga gcgcacaggt cacgtcgccc tggctcggca
     7141 ggatgaagga cgtcggaggg agcggcagga tgtcggtgaa cgacgcgttc accgcatcgg
     7201 acggtccgtg gttgaccacc gagatgtcgt aggtcaggtc gctcccggct gcaggcgtct
     7261 cggtcgcctc ggcaatcttg gagacggaga ggtccgcctg ctgctggacg tccgacacgt
     7321 cggttgctgt gttgtctgat gtgtctggat cgtcgaccgg cgagtcgata ctcgctgtgt
     7381 tggtcagcgt cgcaccggcg tacgccgggt ccagctgtcc ggagatgcga acgactcggg
     7441 tcgctccggc gggcacattg ccgagcgcgc agtcgacagt tgcgttctgc acgcatgacg
     7501 ggtcggactt cgtcgggtcg aacgcgaccg cggtcgggag tacgtcgacc agcgtcaccg
     7561 gcgatgcatc cgacggacct gcgttggtga ccgtgagggt gtagcccacc tccgtgccgg
     7621 caacgagcgg atcggaatca gcggtctttg tcaggctgag gttggcttgg gtaccgatgg
     7681 tcgagtcgaa ggtggccgtg ttgttcgact gttccgggtc tggtgtcgac gtcgtcaccg
     7741 acgccgtgtt cgtgacgact cccgtcgcag cgtcggggtc gacggtaccg gtgatcgtga
     7801 tgactggtgt ctgcaggatg cgcaacaccg ttccgagact gcaggtgacg accgtgcctg
     7861 cgatcgtgca acttccctgg ctcggcaacg cctgggtggc ggtgagcccc gccgggagtg
     7921 tgtcggtgac gaccacgtct gctgcgttcg atgggccggt cgtgttgtcg acggtgatgg
     7981 tgtaactgaa ctccgttccg gccacgagct gcgctgcacc gtctttggca atgtccaggt
     8041 cggcttctgt ccgggtgaat tggaacggcg cttcggctgc gttgttgtcg tggtctgggt
     8101 cggacgtgac ggcggcgacg gtgacgtcgt tcgcaaagcg ggtgttcagc gaagcccccg
     8161 gatctgggat gaccgtgagc agcacctcgc tcgtttcgcc aacgggtaga tcaccgagct
     8221 ggcagaacag gtcgccactc ggatctatct cgcactgatc gaacccttgt gtgccgtcga
     8281 tcgtgatgtc gacaaccccc gctgagagcg atccgatgga gtcgcgcatg ctcacggtgg
     8341 ctgcgtcaga cggaccgtcg ttgcgaacca gcatcctgaa ctgcaggaac tgcccggacg
     8401 ccagtggtgg ttgcgtcagc aactccttgg tgacccggat gtcggccgcc gtctcgatgg
     8461 tcgtggtctc gcgatcggag ttgttcgact cggtcggatc aggggttgtc gacgagaccg
     8521 ccgcctcgtt ggcaatgacg cttgcactgg cgactccggg atcgacgcgg gccgtgatcg
     8581 tgatgtgcac cgtcgagccc gggttcaccg ttccaaggtc gcagctcact gacgaggctg
     8641 gcgtacagga cccctgatcc gtcgtcgctg acacgaccgt gagtcctatg ggaagtgggt
     8701 cggtcaccgt cgtggagtcc gcggccgagg gcccagcgtt ggagactgtc aggttgtaga
     8761 cgacggttcc acctgccaat gcagtggccg gggcagcttt ggtgaccgac acgtcagcgg
     8821 aaacgctggc ggtgctggcg aacgaggcgc tgtcgttgtc gggattcgga tctgtggttc
     8881 cggtcgtgtc cacggttgcg gtattcgtga tggcgccgcc cgggaatgtc gcaatcaccg
     8941 ttgcattgac cgtgaaggcc agcgacccgc caaccgcaag gtctggcgcc gtacaggtga
     9001 cagtctgccc gctgacggag cagcccggcg cggtgatgtc agtcagcgag gtcggcagcg
     9061 tgtcgctgac cgtcacgccc cgggcccgtg aggggccgtt gttggtcgcc gtgagggtgt
     9121 acgagatcgg tccacctgcc a
//
LOCUS       DATBFZ010000433        17074 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_1612775, whole genome shotgun sequence.
ACCESSION   DATBFZ010000433 DATBFZ010000000
VERSION     DATBFZ010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 17074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17074
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1612775"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..505
                     /locus_tag="VH419_13160"
     CDS             <1..505
                     /locus_tag="VH419_13160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044612.1"
                     /translation="ERNYYPLPRIGTGPSNMARVGSYQYFWATWVEALNTTKTTQPSF
                     RIAYNTISAGTVYLHATISDLTIHPGVDGMDNIACGRADVPFDPDAKDTIPQSKGGSQ
                     PSDCWTVYTHSSASEDNETVWLDGTATWHVQVEAPNGTPLAQLGDFHYVVTQRQAVGE
                     VEPLTDW"
     gene            565..3369
                     /locus_tag="VH419_13165"
     CDS             565..3369
                     /locus_tag="VH419_13165"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEY7044613.1"
                     /translation="MVGTRTQTHSRLPGGAPADSRGAEVVRGVLSLLAITLIAIGVPV
                     ALWLGFGPPWPSESPSGDWIYADITSRDVLAILIAVVWLAWLHFCICLVVEFVAERRN
                     RGLAPHIPGGSVGTQPLARRLVGAVLLLSGGVAATVPAAAAAATSNGVHQVSADAAAP
                     STRGGGAAGEAEAPPGQDAHRDRTPAFTDRDAHGLHKYVEVQPPAGRHYDTLWGIAER
                     YLGDGLRYKEIAALNRGVTQPDGTTLRDPDLIYPGWILKLPADAEGPGLRAARHDSQS
                     AAPERSRGGAGTDDPGSRTSPERGDTETSPAGQDGDESTSLVTIGGFSTGGALLAAGL
                     LLNLRRRRGWNGRPHPRGGKPLDHESDLRSSADESSAIFVDTVLRSLAQALPQGATLP
                     APTGAVLGVDGLALTFPAEARTRLDAPWSSDSGARTWLMKRTAAQTLRPEPGALSPLP
                     GVVALGRRADDVETLIDVESIGGVLSISGDLGVARDIAVGLGLALATNRWSDSPRVTF
                     VGFADDLSQLAPGQIRHFDELSAVFETVDAKRRRQHSACAASGFDSVRTGRLSNPDPR
                     LWAPEFIVVSGVPDDADVRRLSDLAGDPRNAIGVIVVGDVKQSPARMVAATDGSLWCG
                     PLGIDVVAHRVTADTYRDALSVFDAELAHGGDGDDPDDPAGAARAVPVVDPDALELET
                     RMPVQITSLGHLSVTAPGELEEARRDLLTELVVYLALHPDGTHPNVLSAAIWPRGVSD
                     EVRDSALAQAATWLGVDASRTPRLSIDAAGRWRLGRSGVRFDWDVFRALANRAAAAAD
                     PTRDLEQALTLATGQAWTGLPTGHYGWLAYETVEADVRVAVVAVARRLAELAAAAGDP
                     MRARTALLAGLRTAPACEDIWRDALTLANRFAGPADVRLVADDMYAALARHGSPRGPE
                     PETDALVDELLPGYRKVSAA"
     gene            3284..3997
                     /locus_tag="VH419_13170"
     CDS             3284..3997
                     /locus_tag="VH419_13170"
                     /inference="COORDINATES: protein motif:HMM:NF041024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acVLRF1 family peptidyl-tRNA hydrolase"
                     /protein_id="HEY7044614.1"
                     /translation="MAHHAALSRRPTLWSTSCCRATGRSPLPESRLVQVPFRRVAGWV
                     QRWDSRHPDSTWTFGSDAVAVESPDGAHATFGVPLGRLERCDLSGLLAHLDQRWRLGV
                     VLVRRGGFAVAYVDGPDVVDVKIGQRHVQGRSKAGGWSQQRFARRRANQAREAFDAAS
                     EHAGRLLAGRAGTLDLLVLGGDRAGVQAVLDHPRLERLSGTPQQWVPVAGDPRRPALD
                     TAVDVARSVQITVVDPQRE"
     gene            complement(4106..5089)
                     /locus_tag="VH419_13175"
     CDS             complement(4106..5089)
                     /locus_tag="VH419_13175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HEY7044615.1"
                     /translation="MGQGYRPYRRATEGNIAMKINPKARIDASKVRVRRGGSRGFPMP
                     GSGGSGGGGFPIPTGGGIGGLVVVVIILVVVTMCSGGIPGTGGGGGDTSASDQLENCQ
                     TGADAADNEQCKLALYVNVIENYWQDALPQQANTDYQHSEMEIFSGSTSSGCGQASSA
                     MGPFYCPADKQVYIDDTFFDDMLQGQLGARGGDFAEAYVVAHEYGHHVQDLLGTMAKA
                     QSRETGPTSPSVRLELQADCYAGIWAHNATTVEDADGNVFITDLTKDDIERAIDAATA
                     VGDDRIQQRSQGHVNEEQWTHGSAQERVHWFMTGYQSGDLDACNTFAADAL"
     gene            5259..6857
                     /locus_tag="VH419_13180"
     CDS             5259..6857
                     /locus_tag="VH419_13180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002550870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEY7044616.1"
                     /translation="MITARHLHIRAGARVLVEDASFFIGPGDKVGLVGRNGAGKTTMT
                     KILAGEAQPVRGEVTRTGSVGYLPQDPRTGDLSVLAQDRILSARGLDDVVRRLRRAEH
                     DMASEDDGVRDKAMHRYARADAELHAAGGFAAESEAAQIAAHLGLPDRVLGQPLQTLS
                     GGQRRRVELARILFSGAETLLLDEPTNHLDADSILWLRDFLKAHRGGLVVISHDVALL
                     DETVTKVFHLDANRTEVDVYNIGWKAYLQQRETDERRRRRERANAEKKAGALMAQADK
                     MRAKASKAVAAQNMARRAERLLDGLEAERRQDKVARIAFPKPASCGRTPLTAEELSKS
                     YGSLEVFTDVDLAVDRGSRVVVLGLNGAGKTTLLRILGGLDDSDTGTVLPGHGLKLGY
                     YAQEHETLDLGRTVLENLQSSAPQLTETQARSVLGSFLFSGDDAYKPARVLSGGEKTR
                     LALATLVVSTANVLLLDEPTNNLDPASREEVLAAIRSYEGAIVLVTHDEGAVQALQPD
                     RVLILPDGVEDLWNEEYADLISLA"
     gene            7255..8064
                     /locus_tag="VH419_13185"
     CDS             7255..8064
                     /locus_tag="VH419_13185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008361919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEY7044617.1"
                     /translation="MTDAYAEAGLRVDLDGPVATVTLCRPETRNAQSPRMWEALADVG
                     ERLGGDRRIVVIRAEGPSFSAGLDTRMFAADGVPGDTSFAELTELDDLALAEQIATYQ
                     EAFSVWRRPDLVSIAAVNGHAVGAGFQLALACDLRVCADDARFCMRETSLGLVPDLGG
                     TQSLVETVGYARALELCVTGRWVEADEARELGLATIVVPADQLEATVADLAAALMAAP
                     HGAVTATKALLRTAAGRSFDDQLAAERSAQVRRLRALTDQPQERASGNKPG"
     gene            8113..9993
                     /locus_tag="VH419_13190"
     CDS             8113..9993
                     /locus_tag="VH419_13190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020577311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY7044618.1"
                     /translation="MMHQLSREDPTKGRDGLTPGTTRRVLAFARPYTAQVMWFLALVI
                     VDALLVVATPLLFKKIVDDGILQSDGALITQLSLIVALIAVGEAALTLAQRYYSSRIG
                     EGLIYDLRTKVFGHVQRMPLAFFSRTHTGSLVSRLNNDVIGAQRAFTSTLSGVVSNVI
                     SLIMVVGFMFVLSWQITLIALLMLPLFLLPAKWAGRKLSGLTREQMQTNAEMSATMTE
                     RFNVGGALLVKLFGRPADEQREFSAKSAKVRDLGVQIAMTGRIFYSALTLVAALATAL
                     VYGVGGHLALRDVVTVGDLVALATLMGRLYGPLTALTNVRIDIMTALVSFERVFEVLD
                     LEPMIQQKPDAVTVDDAPAGVEFRHVDFSYPTADEVSLASLEVTAVQDGRVSKQVLHD
                     VTFGVEPGQMIALVGPSGAGKTTLTHLVARLYDVSAGAVLVGGVDVRDITAASLYDTV
                     GYVTQDAHMFHDTIRANLVYARPGATDAELEDALRSAQVWDLVSSLPDGLDTVVGDRG
                     YRLSGGERQRLAIARLLLKAPRIIVLDEATAHLDSESEVAVQQALDTALEGRTSMVIA
                     HRLSTVRNADQILVVDHGRVADRGTHAELLARGGLYRQLYDTQFASERQAAAMASAAA
                     LS"
     gene            10046..10801
                     /locus_tag="VH419_13195"
     CDS             10046..10801
                     /locus_tag="VH419_13195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020386297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY7044619.1"
                     /translation="MDLGLKDRVYIVTGGTRGLGRATADVLVAEGAKVVIASRDQGHV
                     DAAETALGPAAHGVVADNADPGTPQRLVEAARAAFDRLDGALISVGGPPAGQAMERTE
                     NEWTAAFQSVFLGAARLATLLGRELGDGGAIAFVLSTSVKQPLGGLAISNGLRPGLAM
                     LAKTLADELGPSGIRVNGLLPGSVDTDRVRELDARHDDPAAARARAEAGIPLRRYGRP
                     EEFGRAAAFLLSPAAAYITGAMLPVDGGITRGL"
     gene            complement(10785..11621)
                     /locus_tag="VH419_13200"
     CDS             complement(10785..11621)
                     /locus_tag="VH419_13200"
                     /inference="COORDINATES: protein motif:HMM:NF014193.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HEY7044620.1"
                     /translation="MFNQTNQSNRSNPYRILLSRRWLGLLLVVVVVAFACYELGRWQF
                     HRYDERHDRNAVTRTNERADPVAIDQLMSSTAPPADADEWRRATASGQYDVAHQLVVL
                     YRTREGAHGVDVVTPLVTGSGDAVLVDRGWVATNGNGNEPADVPDPPPGTVTVTGWVR
                     QDDADTGNRVTPSEGSVRSISSRAIGETVPYELYQGWLSLTDEDPTVQPSPERADPPD
                     LSAGPSFFYGLQWWFFALLAIAFWGYFAYAEVHRDPRGEAMAVVRRRRSEVTESNVTD
                     RG"
     gene            11701..12714
                     /gene="moaA"
                     /locus_tag="VH419_13205"
     CDS             11701..12714
                     /gene="moaA"
                     /locus_tag="VH419_13205"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008357067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HEY7044621.1"
                     /translation="MTSSLTDTLPLLDQYGRRASDLRVSLTDRCNLRCSYCMPAEGLD
                     WLPGDDVLTDHEVVRLIDIAVTRLGVDDVRFTGGEPLIRRGLTEIVSRTARFEPRPRI
                     SLTTNGLGLDKTAPALAAAGLDRVNVSLDTVRPDTFAAITRRDRLRDVVRGLEAARDA
                     GLTPVKVNAVLMRGHNDDQAPELLNWCLEHGYELRFIEQMPLDAQHRWSRDEMITADE
                     IFDRLSTAFVLTPHPDERGSAPAELFLVDGGPATVGVIASVTRPFCGACDRVRLTADG
                     QVRNCLFAREESDLRTPLRSGASDDEIADRWRAAMWTKRPGHGIDDESFLQPARPMSS
                     IGG"
     gene            complement(12837..13055)
                     /locus_tag="VH419_13210"
     CDS             complement(12837..13055)
                     /locus_tag="VH419_13210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetone carboxylase"
                     /protein_id="HEY7044622.1"
                     /translation="MTEPPDAGDGQVLICSAKACQRPATTALRWNNPKIHTPDRRKTW
                     LACAEHQESLTAFLAARGFMRETESLDP"
     gene            complement(13052..13369)
                     /locus_tag="VH419_13215"
     CDS             complement(13052..13369)
                     /locus_tag="VH419_13215"
                     /inference="COORDINATES: protein motif:HMM:NF022742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3099 domain-containing protein"
                     /protein_id="HEY7044623.1"
                     /translation="MTQHPEQPVSITSARTSHSDDLHRRQVRYLMSMGIRTICFVLAI
                     VTDGWLRWILVVAALCLPPVAVIIANASDRRRSAGPATFVVPDRAAIGTGRTPDDDAQ
                     PPR"
     gene            13457..13669
                     /locus_tag="VH419_13220"
     CDS             13457..13669
                     /locus_tag="VH419_13220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007077818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin"
                     /protein_id="HEY7044624.1"
                     /translation="MNRTYRVTEVVGTSPDGVDDAIHNAIKRASRTLRHLDWFEVTQV
                     RGYVVDDHIEHFQVGLKLGFRLEDDE"
     gene            13722..14426
                     /gene="fabG"
                     /locus_tag="VH419_13225"
     CDS             13722..14426
                     /gene="fabG"
                     /locus_tag="VH419_13225"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018567714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEY7044625.1"
                     /translation="MSRSVLVTGGNRGIGRAIAQSFVDAGEQVAVTYRSGEPPSGFLG
                     VRCDITDPEQVDSAFATIEEQQGPVEVLVANAGITRDTLLLRMSEDDWSSVIETNLTG
                     TFRVVKRAAKGMLRLRRGRIVLISSVVGLLGSAGQVNYAASKSGLVGMARSIARELGS
                     RSITANIVAPGFVETDMTAELPEDKRTEYLAAIPLGRFTTSDEIASVVRWVASDEAGY
                     ITGAVIPVDGGLGMGH"
     gene            14477..15247
                     /gene="fabI"
                     /locus_tag="VH419_13230"
     CDS             14477..15247
                     /gene="fabI"
                     /locus_tag="VH419_13230"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012921324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEY7044626.1"
                     /translation="MGILDGKRVLVAGVTLDSSIGFATAKVAQEQGATVLISNFGRAL
                     SITKRIAGRLPTTPPVIELDVSDDEHLARLEDEVREHVDGLDGVVHAIAYGNPQTILG
                     GKFLGGPWEDVSHAVRVSAYSYQSLAMACLPLMQPGGSIVGCTFDASVAWPGYDWMGF
                     AKGALESCNRYLARDLGPKGIRCNLVSAGALKTLAAKAIPGFERFEGEWVKRAPLGWD
                     LDDLEPTARTIVALLSDFLPKTTGEIVHVDGGYHAMGA"
     gene            15286..17028
                     /locus_tag="VH419_13235"
     CDS             15286..17028
                     /locus_tag="VH419_13235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012921323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEY7044627.1"
                     /translation="MTPPTTPTGPTGPTGPTGSLIELRMLEGPNLYFPRAAVKLTLDL
                     RALVELSDDAALAFARRIGLTNVRPGAAGSGFRQRFAARAVARLVRQIAAEAGTHRLA
                     VRVRPTTDVHQVVVAFPWRNRRRAEALGRAVAGVLDGLPAADIDTLVGEQAERVRRVE
                     PGAAPTTIKPKVPVIAVTGTNGKTTTSRMIGHIGRQAGLHVGWSSTDGIYFDGELVEA
                     GDYSGPSGAGRVLSQPGVQLAVTETARGGILLRGIGVRHNDVSVVTNISADHLGLQGI
                     DTLDQLAEVKSVVPQITKPSGWAVLNGDDPRVFAMRTSIVARPWVFSRDPSSPSLREV
                     LSSDGRGTTVIDGWICLLTPGGDPDPLVRIVDVPMTLAGLSRFNVENALAAASAALAA
                     GLPRDAVVSGLTTFAPGPELNPGRMNIYSLKDATVIVDLAHNEAGLEALLEVMRGLCR
                     PGGRTLLALGTAGDRTDEIVGSLGEIAARDADVVVIVHKLAYLRGRDQDEMTAIYREA
                     AARVGVHDVPAYDSELEGLEALVARAAPGDVVAVMCHQDREQLDSWLVDQGATVDPPD
                     VLRDKVLLASARTP"
BASE COUNT         2523 a         6004 c         5927 g         2620 t
ORIGIN      
        1 cgagcgcaac tactacccgc tgccgcggat cggcacgggt ccgtcgaaca tggctcgcgt
       61 cggctcctac cagtacttct gggcaacctg ggtcgaggcg ctcaacacca cgaagacgac
      121 tcagcccagc ttccggatcg cctacaacac gatctccgcc gggaccgtct acctgcacgc
      181 caccatcagc gacttgacca tccaccccgg cgtcgacggc atggacaaca tcgcctgcgg
      241 ccgtgccgac gttccgttcg accccgacgc caaagacacc atcccgcaga gcaagggcgg
      301 tagccagccg agtgactgct ggacggtcta cacacactcg tcggcgtcgg aggacaacga
      361 aaccgtgtgg ctcgacggca cggccacctg gcacgtccag gtcgaggcac cgaacgggac
      421 accgttggcg cagctcggcg acttccacta cgtcgtgacc cagcggcagg ctgtcggcga
      481 ggtcgagccg ctgacggact ggtagcgatg accggttccg tgacctctct gtgctccaga
      541 atgaccgtct gaggaggaat gcacgtggtc ggcacgcgta cccaaacgca cagccgcctt
      601 cccggaggag ccccggcgga cagccggggt gccgaggtgg tgcgcggcgt gctctcgctg
      661 ctcgccatca ccctgatcgc gatcggggtg ccggtggcgc tctggctggg cttcgggcca
      721 ccatggccga gcgagtcgcc ctccggcgac tggatctacg ccgacatcac ctcccgtgac
      781 gtccttgcga tcctgatcgc ggtcgtttgg ctggcgtggc tgcacttctg catctgtctg
      841 gtggtcgagt tcgtcgcgga acgacgcaac cggggtctgg cgccgcacat ccccggcggc
      901 tcggtcggga cccagccgct cgcacgccgc ctcgtcggcg ccgtgctgct gctgtccggc
      961 ggtgtcgccg ccaccgttcc cgccgctgct gcggcggcca cgtcgaacgg cgtacaccag
     1021 gtgagcgcgg atgccgcggc cccgtcgacc cgcggcgggg gtgcggcggg agaggccgag
     1081 gcgccgcccg gtcaggacgc gcaccgcgac cgtactcccg cgttcaccga ccgggacgcc
     1141 cacgggctgc acaagtacgt cgaggtgcag ccgccagcgg gccggcacta cgacacgctg
     1201 tgggggatcg cggagaggta tctcggcgac ggcctgcgct acaaggagat cgcggccctc
     1261 aacagggggg tcacacagcc cgacggcacg acgttgcgcg accccgacct catctatccg
     1321 ggatggatcc tcaagctccc cgccgacgcc gagggacccg ggctgcgcgc tgcccgtcac
     1381 gactcgcagt cggcggcgcc ggaacggtca cgtggaggcg ccggcaccga cgatccggga
     1441 tcgcggacca gtcccgagcg tggcgacacc gagacgtccc cagccggcca ggacggcgac
     1501 gagtcgacct ccctggtcac catcgggggg ttcagcaccg gcggtgccct gctggccgcc
     1561 ggcctgctgc tcaacctgcg ccggcgtcgc ggctggaacg gccgtccgca tccccgcggc
     1621 ggcaagccgc tcgaccacga gagcgacctg cgcagttccg ccgacgagtc ctccgcgatc
     1681 ttcgtggaca ccgtgctgcg cagtctcgcg caggcgctgc cccagggggc gacgctgcct
     1741 gcgccgacgg gcgcggtgct gggcgtcgac ggcctggcac tcacgttccc agctgaggcg
     1801 cggaccaggt tggacgcccc ctggtcgagc gactcgggcg cgcgcacgtg gctgatgaaa
     1861 cgcactgcgg cgcagaccct tcgccccgaa cccggcgcgc tgagtcccct gcccggcgtg
     1921 gtcgcgctcg ggcggcgcgc agatgacgtc gagaccctca tcgacgtcga gtcgatcggt
     1981 ggggtgctgt ccatcagcgg cgaccttggc gtggcccgcg acatcgcggt gggactcggt
     2041 ctcgcgctcg cgaccaaccg gtggtcggac tcgccgaggg tcacgttcgt cgggttcgcc
     2101 gacgatctct cccagctggc gccaggccag atccggcact tcgacgagct gtctgctgtc
     2161 ttcgagacgg tcgacgccaa acgccggcgc cagcactccg cctgtgcggc cagcggcttc
     2221 gactccgtcc gaacgggtcg actgtcgaac ccggacccgc ggctgtgggc tccggagttc
     2281 atcgtggtct ccggcgtccc ggacgatgcc gacgtacgcc ggctgtccga tctcgccggt
     2341 gacccacgca acgcgatcgg cgtgatcgtc gtcggtgacg tcaagcagtc accggcgcgg
     2401 atggtcgccg ccaccgacgg ctcgctgtgg tgcggccccc tcggcatcga cgtggtcgcg
     2461 caccgtgtca ccgccgacac ctaccgggac gcgttgagtg tcttcgacgc ggagctcgca
     2521 cacggcggcg acggcgacga ccccgacgac ccggccggcg ccgctcgggc agtacccgtc
     2581 gtcgacccgg acgcactcga gctcgagacc cgcatgcccg tgcagatcac cagcctcggc
     2641 cacctcagtg tgaccgctcc gggtgagctc gaggaggctc gccgcgacct gctgaccgag
     2701 ctcgtcgtct atctggcgct ccacccagac ggcacccacc cgaacgtcct gtccgccgcc
     2761 atctggccgc ggggcgtcag tgacgaggtg cgggactcgg cgctggcgca ggccgccacc
     2821 tggctgggcg tcgacgcctc ccggacgccg cggctgtcga tcgacgccgc cggccggtgg
     2881 cggctcggcc ggtcgggagt ccggttcgac tgggacgtgt tccgtgcgct cgccaaccgc
     2941 gccgccgctg ccgcggaccc gactcgtgat ctcgaacagg cgctcacgct ggccaccggt
     3001 caggcctgga ccggcctccc gaccggacac tacggctggc tcgcctacga gaccgtggag
     3061 gcggatgtcc gcgtcgcggt cgtggccgtg gcgcggcggt tggccgagct ggccgccgcg
     3121 gcgggcgacc cgatgcgggc acgtacggcc ctgctcgcgg ggcttcggac agcgccggcg
     3181 tgcgaggaca tctggcgaga cgcgctcacg cttgccaacc ggttcgccgg cccagccgac
     3241 gtacggctgg tggccgacga catgtacgcc gcgctggccc ggcatggctc accacgcggc
     3301 cctgagccgg agaccgacgc tctggtcgac gagctgctgc cgggctaccg gaaggtctcc
     3361 gctgcctgag tcgcggctgg tccaggtccc gttccggcgt gtagccggct gggtgcagcg
     3421 gtgggacagt cggcaccccg acagcacctg gaccttcgga tcggatgcgg tggccgtcga
     3481 gagcccggat ggcgcacacg cgacgttcgg tgtgccgctc ggccgcctcg aacgttgcga
     3541 cctgtccggc ctgctggcgc acctcgatca gcggtggcgg ctcggtgtcg tcctggtgcg
     3601 ccgcgggggc ttcgcggtcg cgtacgtcga cgggcctgac gtcgtcgacg tgaagatcgg
     3661 tcagcgtcac gtgcagggac gctccaaggc cggcggctgg tcgcagcagc gattcgcccg
     3721 ccgccgcgcc aaccaggctc gagaggcgtt cgacgcggcg tctgagcatg ccggtcggct
     3781 gctcgcgggc cgcgccggca cgctggacct gctcgtcctg ggtggagacc gggccggcgt
     3841 acaggcggtt ctggaccatc cgcggctgga gcggctgtcg ggcacacctc agcaatgggt
     3901 gcctgtcgca ggcgacccac gccggccggc actggacacc gcggtcgacg tggcgcggtc
     3961 ggtgcagatc acggtcgtgg atccgcaacg ggagtgaccc tccgccgcca agcccgatca
     4021 gcgggtgccg aggtggcaag cgcgcagcga cacgccggca gaactgaacg aaacctgcca
     4081 cctcggccgt gccagggtcg cgggctcaga gggcgtcggc ggcgaaggtg ttgcaggcgt
     4141 cgagatcgcc gctctggtag ccggtcatga accagtggac gcgctcctgc gcggagccgt
     4201 gtgtccactg ctcctcgttg acgtggccct gggaacgctg ctggatccgg tcgtcaccga
     4261 ccgccgtggc tgcgtcgatc gcgcgctcga tgtcgtcctt ggtcaagtcg gtgatgaaga
     4321 cgttgccgtc ggcgtcctcc accgtcgtcg cgttgtgcgc ccagatgccc gcgtagcagt
     4381 ccgcctgcag ctccagccgc accgacgggc tggtcgggcc ggtctcgcgg ctctgggcct
     4441 tggccatcgt gccgagcagg tcctggacgt ggtgcccgta ttcgtgggcg acgacgtacg
     4501 cctccgcgaa gtcgccaccg cgggcaccga gctgaccctg gagcatgtcg tcgaagaacg
     4561 tgtcgtcgat gtacacctgc ttgtcagccg ggcagtagaa cggccccatc gccgacgacg
     4621 cctgaccgca cccgctggat gtgctgccgc tgaagatctc catctcggag tgctggtagt
     4681 cggtgttggc ctgctgagga agcgcgtcct gccagtagtt ctcgatcacg ttcacgtaca
     4741 gggccagctt gcactgctcg ttgtcggccg cgtccgcacc ggtctggcag ttctccagct
     4801 ggtcgctcgc cgacgtgtca ccgccaccgc ctccggtgcc aggtatgccg cccgagcaca
     4861 tcgtcacgac gacgaggatg atcaccacga cgacgagtcc accgataccg ccaccggtcg
     4921 ggatggggaa cccgccaccg ccactgcccc cgctgcccgg catggggaag ccgcgcgaac
     4981 ccccgcggcg gacccggacc ttgctcgcgt cgatccgggc cttggggttg atcttcatcg
     5041 cgatattccc ttcggtggct cgccggtagg gacggtaccc ttgccccatg gcgaggggac
     5101 ctgtgctgac ccgacatccg cggtcggcgt catgaggaca acgggctagg agccattctc
     5161 cctgccggtg gccggttccc cgatctggcc actccattcc ttgtccgggt caaccacatc
     5221 catcctctgt ccgctctgca tcgaaagcca gtcgagccat gatcaccgca cgccacctcc
     5281 acatccgcgc gggtgcgcgg gtgctggtcg aggacgcgtc gttcttcatc ggcccgggag
     5341 acaaggtcgg gctggtcggt cgtaacggcg ccgggaagac cacgatgacg aagatcctcg
     5401 cgggggaggc gcagcctgtt cgcggtgagg tgacgcgcac cggctctgtc ggctacctgc
     5461 cgcaggaccc gcggaccggc gacctctcgg tgctggcaca ggaccgcatc ctgtccgctc
     5521 gtggcctcga cgacgtcgta cgccggctgc gccgggccga gcacgacatg gccagcgagg
     5581 acgacggcgt acgtgacaag gccatgcatc gctacgcccg cgccgacgcc gagctgcatg
     5641 cggcgggggg cttcgccgcc gagtcggagg cggcgcagat cgcggcccac ctcggtctgc
     5701 cggaccgggt gctcgggcaa ccgctgcaga ccctgtccgg cggtcagcgg cgccgggtcg
     5761 agctggcccg gatcctgttc tcgggcgctg agacgttgct gctcgacgag ccgacgaacc
     5821 atctcgacgc cgactcgatt ctgtggctcc gtgacttcct gaaggcgcac cgaggtggcc
     5881 tggtcgtgat cagccacgac gtggcactgc tcgacgagac cgtgacgaag gtcttccatc
     5941 tggacgcgaa ccggaccgag gtcgatgtct acaacattgg atggaaggcc tacctgcagc
     6001 agcgggagac cgacgaacgg cgccgccgac gcgaacgtgc caacgccgag aagaaggcgg
     6061 gcgcgctcat ggcgcaggcc gacaagatgc gcgcgaaggc cagcaaggcg gttgctgcgc
     6121 agaacatggc ccggcgggcg gagcggttgc tcgacggcct ggaggcggag cgccgccagg
     6181 acaaggtcgc ccggatcgct tttccgaaac ccgcgtcgtg cggccgcacg ccgttgaccg
     6241 ccgaggagct gtcgaagtcc tacggttcgc tggaagtctt caccgacgtc gaccttgccg
     6301 tcgaccgcgg tagccgggtc gtcgtcctcg gcctgaacgg cgccgggaag acgacgctgc
     6361 tgcggatcct gggcggcctc gacgactccg acaccgggac cgtcctgccc ggccacgggc
     6421 tcaagctggg ctactacgcg caggagcacg agacgctgga cctcggccgc accgtgctgg
     6481 agaacctcca gtcgtccgcg ccgcagctga ccgagacgca ggcgcgcagc gtcctcggct
     6541 ccttcctgtt ctccggcgac gacgcgtaca agccggcgcg ggtgttgtcc ggcggtgaga
     6601 agacccgcct cgcgctggcc accctggtcg tgtcgacggc caacgtcctg ctgctggacg
     6661 agccgaccaa caatctcgac cctgccagcc gcgaggaggt gctcgccgcg atccggtcgt
     6721 acgagggcgc gatcgtgctc gtcacgcacg acgagggcgc ggtccaggcg ctgcagcccg
     6781 atcgcgtgct gatcttgccg gacggggtcg aggacctgtg gaacgaggag tacgccgacc
     6841 tcatctccct cgcctgatcc acgcgttgca gccacctgcc agctgtgttt gccgagacat
     6901 ctctggaact ggcttgctct ccaggtggat ccggtgaccc ctcaggttgc gttgtccgga
     6961 cacggccgcg atgggtatgc gtgctcgtcc ggcgtaccgt cgtcatgggc tgaggtgaat
     7021 cggcattgac gagccggagg ctgtggggtc ctccgctgcc ggcccgcgtt tcgtccctgg
     7081 tgctctgtgt gactggcgtc ggacacgcaa ctctcagcct ccgcggtttg tgatgttcac
     7141 atccgagacc cactgtgccc atcggaggtt tgcgatgttc acacgccggt tgggctgtaa
     7201 acatcgcaaa ccaccagggg tcaggacgtc caacgggggc tctatccttc ggccatgacg
     7261 gatgcgtacg ccgaggccgg gttgcgggtc gacctggacg gtccggtggc aacggtcacg
     7321 ctgtgccggc ccgagacgcg caacgcacag tcgccacgga tgtgggaggc actcgcggac
     7381 gtcggcgagc gactcggcgg tgaccggcgc atcgtcgtca tccgggccga ggggccgtcg
     7441 ttcagtgccg ggctcgacac gcggatgttc gctgccgacg gagtcccggg cgacacgtcg
     7501 ttcgccgagc tgaccgagct cgacgacctc gccctggccg agcagatcgc gacctaccaa
     7561 gaggcgttct ccgtctggcg ccggcccgac ctcgtcagca tcgccgcggt gaacggacat
     7621 gcggtcggcg cgggattcca gctcgctctc gcctgcgacc tccgggtctg cgccgacgat
     7681 gcccgcttct gcatgcgcga gacgtcgctc ggtctcgtcc cggacctcgg cggcacccag
     7741 tcgctcgtcg agacggtcgg ctacgcccgc gccctcgagc tgtgcgtgac cggtcgctgg
     7801 gtcgaggccg acgaggctcg cgagctcggc ctggcaacga tcgtcgtacc cgcggaccag
     7861 ctcgaggcga ccgttgcgga cctggccgcg gcgctcatgg cggctccgca cggagccgtc
     7921 accgcgacga aggcgttgct ccggaccgca gccggacgca gcttcgacga ccagctcgcc
     7981 gccgaacgct cggcccaggt acgccgcctt cgcgcgctga ccgaccagcc ccaagaacgg
     8041 gcgtccggga acaaaccggg ctgaggagat gttgcaggtg gcaccatgag cgttcctccg
     8101 agcccgatgc ggatgatgca tcagctctct cgggaggacc cgacgaaggg tcgcgacggg
     8161 ctgacccccg ggacgactcg acgggtcctg gcgttcgcgc gcccgtacac ggctcaggtc
     8221 atgtggttcc tggccctggt gatcgtcgac gcgctgctcg ttgtcgccac gccgctgctg
     8281 ttcaagaaga tcgtcgacga cggcatcctc cagtccgacg gcgcgctcat cacccagctc
     8341 tcgctcatcg tggcgctcat tgccgtcggc gaggccgccc tcaccctggc gcagcgctac
     8401 tactcgtccc ggatcggtga gggactgatc tacgacctgc gcacgaaggt cttcggccac
     8461 gtgcagcgga tgccgctcgc gttcttctcc cgcacgcaca ccgggtccct cgtgtccagg
     8521 ctcaacaacg acgtgatcgg tgcgcagcgg gcgttcacgt ccacgctgtc cggcgtggtc
     8581 agcaacgtga tcagcctgat catggtcgtc ggcttcatgt tcgtgctgtc gtggcagatc
     8641 acgctgatcg cgctcctcat gctgccgctg ttcctcctgc cggcgaagtg ggcgggccgc
     8701 aagctgtcgg gcctcacccg cgaacagatg cagacgaacg ccgagatgtc cgccacgatg
     8761 accgagcgct tcaacgtcgg cggcgccctg ctggtcaagc tcttcggtcg cccggccgac
     8821 gagcagcgcg agttctccgc caagtcggcg aaggtgcgtg acctcggcgt ccagatcgcg
     8881 atgaccggac gcatcttcta cagcgcgctc accctcgttg ccgccctcgc caccgcgctc
     8941 gtgtacggcg tcggaggtca tctggccctg cgcgacgtcg tcacggtcgg cgacctcgtc
     9001 gcactcgcca cgctgatggg acgcctgtac ggaccactca ccgccttgac gaacgtccgg
     9061 atcgacatca tgaccgcgct ggtctcgttc gagcgggtct tcgaggtgct cgacctggag
     9121 ccgatgatcc agcagaagcc ggatgccgtg accgtcgacg acgcccctgc cggcgtcgag
     9181 ttccggcacg tggacttctc ctacccgacc gccgacgagg tgtcgctggc cagtctcgag
     9241 gtgaccgcgg ttcaggacgg ccgtgtcagc aaacaggtcc tgcacgacgt gacgttcggc
     9301 gtcgagcccg gccagatgat cgcgctcgtc gggccgtccg gggccggcaa gaccacactg
     9361 acgcacctgg tggccaggct gtacgacgtc agcgcgggcg ccgtcctcgt cggtggtgtc
     9421 gacgtgcgcg acatcaccgc cgcgtcgctg tacgacacgg tcggctacgt cacgcaggac
     9481 gcgcacatgt tccacgacac gatccgtgcc aacctggtct acgcgcgtcc cggcgccacc
     9541 gacgccgagc tcgaggacgc gctgcggtcc gcccaggtct gggacctggt gtcctcgctt
     9601 ccggacggtc tcgacacggt cgtcggtgac cgcggctacc gcctcagcgg cggagaacgg
     9661 cagcggctcg cgatcgcccg actgctgctc aaggctcccc gcatcatcgt gctcgacgag
     9721 gccaccgccc acctcgacag tgagtccgag gtcgcagtcc aacaggcact ggacaccgcc
     9781 ttggagggcc gtacgtcgat ggtgatcgcg caccgacttt cgaccgtccg caacgccgac
     9841 cagatcctcg tcgtcgatca cggccgggtc gccgaccgcg gcacccacgc cgagctgttg
     9901 gcgcgcggtg ggctgtaccg ccagctctac gacacccagt tcgccagcga gcggcaggct
     9961 gcggcgatgg cgtcggcggc ggcgctgtcc tgatcgaggt tcgcctcgac tgcaccagcc
    10021 gcgcgacgcg ggcagaatgg ccgccatgga tctgggcctg aaggatcgtg tctacatcgt
    10081 caccggcggc acacgtggtc tcggtagggc caccgccgac gtgctggtcg cggagggcgc
    10141 gaaggtggtg atcgcgtcgc gcgaccaggg ccacgtcgac gcggcagaga ccgcgctcgg
    10201 tcctgccgcg cacggcgtcg tcgccgacaa cgccgatccg ggtacgccgc aacggctggt
    10261 cgaggccgcc cgcgccgcct tcgaccgcct cgacggcgcg ttgatcagtg tcggcggtcc
    10321 accggcaggc caggcgatgg aacgcaccga gaacgagtgg acagcggcct tccagagcgt
    10381 gttcctcggc gcggcccggt tggcgacgct gctcggccgc gagctgggcg acggtggcgc
    10441 gatcgccttc gtgctctcga cgtcggtcaa gcaaccgctc ggcgggctgg cgatctccaa
    10501 cggccttcgc ccgggactcg ccatgctcgc caagacgctc gccgacgagc tcggtccctc
    10561 gggtatccgg gtgaacggct tgctcccggg cagcgtcgac accgaccgcg tccgcgagct
    10621 cgatgcccgg cacgacgatc ccgcggccgc ccgtgcgagg gcagaggcag gcatcccgct
    10681 gcggcgctac ggaaggccag aggagttcgg ccgtgccgcg gcgttcttgc tgtcacccgc
    10741 cgcggcgtac atcaccgggg cgatgctgcc cgtggacggc gggattaccc gcggtctgtg
    10801 acgttcgact cggtgacctc ggatcgcctg cggcgcacga cggccatggc ctcaccacgc
    10861 gggtcacgat gcacttctgc ataggcgaaa tagccccaga acgcgatggc gagcagcgcg
    10921 aagaaccacc actggagccc gtagaagaag ctgggcccgg cgctcaggtc aggtgggtcg
    10981 gcacgctccg gcgacggctg gacggtcggg tcctcgtcgg tgagggacag ccagccctgg
    11041 tagagctcgt acgggacggt ctcgccgatg gccctcgacg aaatcgaccg gaccgagcct
    11101 tcagacggtg tcacccggtt gccggtgtcg gcgtcgtcct gccgtaccca gccggtcacc
    11161 gtcacggtgc cgggcggagg gtccggtacg tcggccggct cgttcccgtt gccgttcgtc
    11221 gcgacccagc ctcggtcgac gagcacggcg tcgcccgagc cggtgacgag cggcgtgacg
    11281 acgtcgacac cgtgcgcgcc ctcgcgggtc cgatagagca cgaccagctg gtgggcgacg
    11341 tcgtactggc ctgacgccgt cgcgcgccgc cactcgtcag catcggccgg gggcgccgtg
    11401 ctgctcatca gctggtcgat cgcgaccggg tccgcgcgtt cgttcgtccg ggtgacggcg
    11461 ttccggtcgt gccgctcgtc gtaccggtgg aactgccacc ggcccagctc gtagcacgcg
    11521 aacgccacga ccacgaccac cagcagcaac ccgagccagc gccggctcag gaggatgcga
    11581 taggggttcg accggttgga ctggttcgtc tggttaaaca tgaggcaccg ccgccaacgt
    11641 aacccagcgc atccgcacca cgatgcggcg gccctgccgg cgaccccgta ggctgacctg
    11701 gtgacgagtt cgttgaccga cacgctgcca ctcctcgacc agtacggtcg ccgggccagc
    11761 gacctccggg tgtccctcac cgaccgctgc aacctgcgct gctcgtactg catgccggcc
    11821 gagggactcg actggctgcc gggcgatgac gtgctcaccg accacgaggt cgtgcggctg
    11881 atcgacatcg cggtcacccg gctcggcgtc gacgacgtcc ggttcaccgg tggcgagccg
    11941 ctgatccggc gcgggctgac cgagatcgtc agcaggacgg cgaggttcga gccacggccg
    12001 cggatctcac tgacgacgaa cgggctgggc ctcgacaaga cggcgccggc gctcgcggcc
    12061 gcggggctcg accgggtgaa cgtgagcctc gacaccgtgc gccccgacac gttcgccgcc
    12121 atcacccgtc gggaccgact ccgcgacgtc gtgcgcgggc tcgaggcggc ccgagacgca
    12181 ggcctcacgc cggtgaaggt caacgcggtg ctgatgcgcg gccacaacga cgaccaggcg
    12241 ccggagctgc tgaactggtg cctcgaacat ggctacgagc tgcgcttcat cgagcagatg
    12301 ccgctggacg cccagcaccg gtggagccgg gacgagatga tcaccgcgga cgagatcttc
    12361 gaccggctca gcaccgcttt cgtgctgacg ccgcatccgg acgaacgcgg cagcgccccc
    12421 gccgagttgt tcctcgtcga cggtgggccg gcaaccgtcg gcgtcatcgc ctcggtgacc
    12481 cgccccttct gtggggcgtg cgaccgcgta cggctgactg ccgacgggca ggtccggaac
    12541 tgcctgttcg cacgtgagga gtccgacctg cgtacgccgt tgcgctccgg cgcgagcgac
    12601 gacgagatcg ccgaccggtg gcgggcggcc atgtggacga aacgccccgg ccacggcatc
    12661 gacgacgagt cgttcctcca gccggcccgc ccgatgagct ccatcggcgg ctgaccgacc
    12721 ccgctgggtc cccgcacccc ggcgggtccc cgcgatttgc gggaaacatc aggttttcgt
    12781 gccccgagac gcagcacctt tcccgcaatt cgcggaaacc cggcagggcg ggagggtcag
    12841 gggtcgaggg actcggtctc gcgcatgaag ccacgggcgg cgagaaaggc cgtgagtgac
    12901 tcctggtgtt cggcacacgc aagccacgtc ttgcgacgat ccggggtgtg gatcttcggg
    12961 ttgttccacc gaagtgcggt cgtagccgga cgttgacatg ccttcgccga gcagatgagg
    13021 acctggccgt ccccagcgtc cggtggttcg gtcatcgagg cggttgtgcg tcgtcgtccg
    13081 gcgtccggcc cgtgccgatc gcggcccggt cggggacgac gaacgtggcc ggtccggcag
    13141 aacggcgccg gtcgctggcg ttggcgatga tgacggccac cggaggcagg cagagcgcgg
    13201 cgaccacgag gatccagcgc agccagccgt cggtcacgat cgccagcacg aagcagatgg
    13261 tgcggatgcc catcgacatc aggtaccgca cctggcgtcg gtgcaggtcg tcgctgtggc
    13321 tggtgcgcgc cgacgtgatg ctgacgggtt gctccgggtg ctgggtcacg gttggctggt
    13381 ctcctcacgt ccaccgtacg ccgcgaacag atccgggaca ccatccggct tcgcgcaagg
    13441 caggaggtca cccagcatga accgcacgta ccgcgtcacc gaggtcgtcg gcacatcgcc
    13501 ggacggtgtc gacgacgcca tccacaatgc gatcaagcgg gccagccgaa cccttcgaca
    13561 cctcgactgg ttcgaggtga cgcaggtgcg cggctatgtc gtcgacgacc acatcgagca
    13621 cttccaggtc ggtctcaagc tcggcttccg cctggaggac gacgagtgag ccccggtcgt
    13681 cgcctcgtcg tacccagccg tacgcggtag cgtcaggcgc cgtgagcaga tccgtactcg
    13741 tcaccggcgg caaccgtggg atcggcaggg cgatcgccca gtcgttcgtc gacgccggcg
    13801 agcaggtcgc cgtcacctac cgctcgggcg agccgccgtc agggttcctc ggcgtgcgct
    13861 gtgacatcac cgaccccgag caggtggact cggcgttcgc cacgatcgag gagcagcagg
    13921 gaccggtcga ggtgctcgtc gccaacgccg gcatcacccg cgacaccctg ctgcttcgga
    13981 tgtccgagga cgactggtcc tcggtcatcg agacgaacct gaccgggacg ttccgcgtcg
    14041 tgaagcgggc ggcgaagggc atgctccggc tgcgccgagg tcgcatcgtg ctgatctcgt
    14101 ccgtggtggg actgctcggt tctgccggcc aggtcaacta cgcggcgagc aagtcagggc
    14161 tggtcgggat ggcgcgctcc atcgcccgcg agctcggcag ccgttcgatc acggcgaaca
    14221 tcgtcgcccc agggttcgtc gagaccgaca tgacggcgga gctaccggag gacaagcgga
    14281 cggagtacct cgccgccatc ccgctcggcc ggttcaccac gagcgacgag atcgcgtcgg
    14341 tggtgcgctg ggttgcctcc gacgaggccg gttacatcac cggggccgtc atcccggtcg
    14401 acggagggct gggcatgggt cactgacgcg ggctggcgcc gaaacgaccg agcgggtacg
    14461 acgaaaggct ggacacatgg gaatcctcga cggcaagcgc gtcctcgtcg ccggcgtgac
    14521 gctcgactcc tccatcggct tcgcgaccgc gaaggtggcg caggagcaag gcgccaccgt
    14581 gctgatctcg aacttcggcc gggcgctgtc gatcacgaag aggatcgccg gccggctccc
    14641 caccacaccg ccggtgatcg agctcgacgt cagcgacgac gagcacctgg cccggctgga
    14701 ggacgaggtg cgtgagcacg tcgacggtct ggacggggtc gtccacgcga tcgcgtacgg
    14761 gaacccgcag acgatcctcg gcggcaagtt cctcggcggt ccctgggagg acgtgtcaca
    14821 tgccgtgcgg gtctcggcgt actcctatca gtccctcgcg atggcctgcc tgcccctcat
    14881 gcagccaggc ggcagcatcg tcggctgtac gttcgacgcc tcggtcgcct ggccgggata
    14941 cgactggatg ggcttcgcca agggcgcgct cgagtcgtgc aaccggtacc tggcccgcga
    15001 cctggggccg aaaggcatcc gctgcaacct cgtgtcggcc ggtgcgctga agacccttgc
    15061 cgccaaggcg attccaggct tcgagcgttt cgagggcgag tgggtcaagc gcgcaccgtt
    15121 aggctgggac ctcgacgacc tcgagccgac cgcgcggacg atcgtggcgc tgttgagcga
    15181 cttcctgccg aagacaacag gcgagatcgt ccacgtcgac ggtggctacc acgccatggg
    15241 cgcctgacca cgcacgacgc atcgggtcgg ctgtagcgtg acgacgtgac ccccccgacc
    15301 acgccgaccg ggccgaccgg gccgaccggg ccgaccgggt cgctgatcga gctccggatg
    15361 ctcgaggggc cgaacctgta cttcccgcgt gcggcggtca agctcacgtt ggacctgcgt
    15421 gcgctcgtcg agctctccga cgacgcggca ttggcgttcg cccgccggat cggcctcacg
    15481 aacgtgcgtc ccggtgcagc cggttctggg ttccggcagc gcttcgcggc tcgcgccgtc
    15541 gccaggttgg tgcgccagat cgcggcggag gcgggtacgc accggttggc ggtccgggtg
    15601 cgtcccacca ccgacgtcca ccaggtcgtt gtcgcgttcc cgtggcggaa ccgccgccgt
    15661 gccgaggcgc tcggccgcgc ggtggcgggt gtcctcgacg gtctccccgc ggccgacatc
    15721 gacacgctgg tgggggagca ggcggagcgg gtacgccgcg tggagccggg cgccgcaccg
    15781 acgaccatca agccgaaggt ccccgtcatc gccgtgaccg gcaccaacgg caagacgacg
    15841 acgtcccgga tgatcggcca catcggacgg caggccggac tccacgtcgg gtggtcgtcg
    15901 accgacggga tctacttcga cggtgagctc gtcgaggccg gcgactactc cggtcccagt
    15961 ggcgccggtc gcgtactgag ccagcccggc gtacagctcg ccgtcaccga gacagcccgc
    16021 ggcggcatcc tgctgcgcgg catcggcgtg cggcacaacg acgtctcggt ggtgacgaac
    16081 atcagcgcgg accacctcgg gctccaaggg atcgacacgc tcgaccagct ggccgaggtc
    16141 aagtccgtcg tgccgcagat cacgaagccg agtggctggg cggtgctcaa cggcgacgat
    16201 ccgcgcgtct tcgccatgcg gacgtccatc gttgcgcgcc cctgggtgtt cagccgcgac
    16261 ccgtcctcgc cgagcctgcg cgaggtgctg agctcggatg gccgcgggac cacggtcatc
    16321 gacggctgga tctgcctgct gacgcctggc ggtgatcccg atccgctggt ccggatcgtc
    16381 gacgtaccga tgacgttggc ggggttgagc cggttcaacg tcgagaacgc gcttgcggcg
    16441 gcatccgcgg cgctggcggc cgggctaccg cgcgacgccg tcgtgtccgg actgacgacg
    16501 ttcgcgcccg gtcccgagct caacccgggc cggatgaaca tctactcgct gaaggacgcc
    16561 acggtcatcg tggacctcgc gcacaacgaa gccggcctcg aggcactgct cgaggtgatg
    16621 agggggctgt gtcgaccagg cgggcgcacg ctgctggcac tcgggactgc cggggacagg
    16681 accgacgaga tcgtcggcag cctcggggag atcgcggcgc gcgacgccga cgtcgtcgtg
    16741 atcgtgcaca agctggccta cctgcgcggg cgggaccagg acgagatgac ggcgatctat
    16801 cgggaagcgg ccgcccgggt cggggtccac gacgtaccgg catacgactc agagctcgag
    16861 ggtctggagg cgctggtggc gcgggctgca cccggtgacg tggtcgcggt gatgtgtcac
    16921 caggaccgcg agcagctgga ctcctggctc gtcgaccagg gcgccaccgt ggacccaccc
    16981 gacgtcctcc gcgacaaagt cctcctcgcc tccgcccgca ctccctgacg gcgcgtgggt
    17041 ttgcgatgtt tacagccctt tcgggctgta aaca
//
LOCUS       DATBFZ010000434        13142 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_2592710, whole genome shotgun sequence.
ACCESSION   DATBFZ010000434 DATBFZ010000000
VERSION     DATBFZ010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 13142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13142
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2592710"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(137..1645)
                     /locus_tag="VH419_13240"
     CDS             complement(137..1645)
                     /locus_tag="VH419_13240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020574447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HEY7044628.1"
                     /translation="METATATTEPALRVGPVEQRLFGSFVEHMGRCVYTGIFEPGHPR
                     ADADGFRRDVLDLVRELGPTVVRYPGGNFVSSYRWEDGIGPPQQRPRRLDLAWRTIES
                     NAFGVDEFMAWCAAVGTEPMMALNLGTRGVESAVDLVEYCNVPRGTMWSDLRRSHGRA
                     EPYAVKLWCLGNELDGDWQVGHKTAHEYGRLAAETARAMRMVDPSVELVACGSSYAEM
                     PTFGSWEETVLGECYDLVDSVSLHAYYEPEGDDLDSFLASSVHLERAIDAVVTVADRV
                     RAEVGSPKRLRLSVDEWNVWYRQNFPSGGLDWAESRSLIEDSYDMVDAVVVGSLLIAL
                     LRHVDRVGIACQAQLVNVIAPILTEAGGSAWRQTTFHPFAQVTRYASGDVLRVQTRCP
                     VQETSKHGEVPLLDAVATYDGEPGGPGGLVVFAVNRSRSEPIKLDLDLRAFDGLDRVE
                     ATTLTHDDPHARNVAADPDRVIPRPLADVRLDSDHLDAVLPTLSWNVLRLTG"
     gene            1855..2580
                     /locus_tag="VH419_13245"
     CDS             1855..2580
                     /locus_tag="VH419_13245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044629.1"
                     /translation="MSSTVRDRCAAATGALYVVLVIVGNELYTRGTHQSAHPSGDQVL
                     ADARHIASSSMAHVGFAMEGLGFVALVAFLGYLGDVLIRRRREQVASNVAAVIAMVAG
                     ITGVAIKLGSAVPTAALMFDHKTLSPELAQLLNDMSTVGWVLSWLPFVIFVAAGGWAL
                     HRCSLVGKPTAYVALVIGVVGIPLAVLGLEDLANLNPIAFLLGLLWTLVVSIRLAVRP
                     GVVRASEPYDATVSTGMPVGAAN"
     gene            2625..4649
                     /locus_tag="VH419_13250"
     CDS             2625..4649
                     /locus_tag="VH419_13250"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEY7044630.1"
                     /translation="MIRWGIAKDPAAWAWVAVVAAIVDTVALGLMGVVIAVEDIQTRI
                     VQDGAFINLVAGPTFPLLAALMLRHSDRDTPRRQDRLAWLFLGLGVLCTATGVVFVTA
                     AYGLRHDLPFTQAAVWVEGWLWTFVGPGVILLLLWFPTGDVPGSRWRWAVRGVVAANV
                     CLWMAAAFMPGSYSDFTADVHNPVGWSGGEPLLQVLGGIGQALLAATFFAALASLAVR
                     YRRGDERVRSQLRWLLTAVVVIAVTIAVPTPESIDPVVLVLNVLATALLPLTLAVALT
                     RGGYALPRVLVYGMLSILLLIAYLAVIGVADAAFGSGADRAATLAAAGLIAVLVAPLR
                     ARLQQSVDRLVYGGRGDPYEALTDFGRRIAGSPDDLLAQVVRVVSEALRAPYVAVELP
                     GDITPTTSVGNAVEDSVAMPLSLRGQNVGRLVVGRRDRREQYGRRDMLLLEDLARHIA
                     VAAHAAVLTKDLQRSRESLVVAREEERRRIRRDLHDGLGPALAGVAFGLDAARNTLRV
                     DLTSTDRALAELKTELQASIADVRRLVYDLRPPALDQLGLVPALEEYAARLRERATLD
                     ISVTSPALPALPAAVEVAAYRIATEALTNVARHSRARWTTVAFALEDSCLRLQVLDNG
                     VGVASANGSTGVGLAAMAERAAELGGSCRVTARSGGGTSVVAELPLLVAL"
     gene            4646..5290
                     /locus_tag="VH419_13255"
     CDS             4646..5290
                     /locus_tag="VH419_13255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY7044631.1"
                     /translation="MSTRVLLVDDHPTFRRGLGALLESLPDIEVVAEAGDGEAAIALA
                     TSLQPDVVMMDLNMPELNGVEATRRLVAANPDVAVLVLTMLDDDEAVFGAMRAGARGY
                     IVKGADTDDVVRALESVARGDAVFGPAVASRVLSYLTRPLSARDPMLFPELSEREREV
                     LELMARGLTNSEIARRLVVSPKTVRNHASNVFTKLQVSDRSEAILRARNAGLGS"
     gene            5377..6273
                     /locus_tag="VH419_13260"
     CDS             5377..6273
                     /locus_tag="VH419_13260"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HEY7044632.1"
                     /translation="MTVTPIRYVSDDEDSARWLSFPFRDGDIVISTRSKSGTTWMQMI
                     CALLVFQSPDLPEPLATLSPWLDWKIAPIGEVIGRLEAQQHRRFVKTHTPLDGVPQDG
                     RATYIVVARDPRDMYVSLYHQGDNIDRDAVRRLLGQPERAPDDVRPERPPLDQALEAW
                     IGRDDSPQQSMDSIRGVFHHVTDAWRRRETQSNVLLVHYDDLLHDLDGQMRDLAARLG
                     LDVAEKRWPELVDAATFTTMRERASAVVPDTAGVLKDANAFFRRGSSGAWRDVMTAGQ
                     VEHYWRRVDGLAPPEVVAWLHR"
     gene            6294..7190
                     /locus_tag="VH419_13265"
     CDS             6294..7190
                     /locus_tag="VH419_13265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013161177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HEY7044633.1"
                     /translation="MPTTVADLLDLLELEQLEEDLYRGRQPDTAMQRVFGGQVAGQAL
                     RAAWHTIGAGLAVHSLHSYFLRPGDTSVPIIYDVERIRDGRSFATRRVVARQHGKSIF
                     YLTASFHIEEPGFDHQDAMPDVPDPEDCLSMGDLFRRITGRDDDTSWEREWAALDVRY
                     AADSRPGGALQNAQQPARTQIWVRAADKVPDEPVIDTCVLTYVSDLTLLGVSLVPHGV
                     IIASRQIQPASLDHSMWFHRPFRSDEWMLYDQHSPSATGARGFALGTVFSRDGVLVAS
                     VAQEGLIRPIGDARDRLLRSAD"
     gene            complement(7198..8004)
                     /locus_tag="VH419_13270"
     CDS             complement(7198..8004)
                     /locus_tag="VH419_13270"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY7044634.1"
                     /translation="MGRGILSVDPDDHDESQAAATSERMRPHRGAHAQRTAQSHAAHL
                     LPHLRPGMRLIDLGCGPGSITVGLAAAVTPGLTVGVDVDAVPVNGVAVVVGDVRRLPF
                     PDASFDAIFASAVLQHVAEPADVLREARRVARTGAVIALVDADWDGELLHPTNAVLRR
                     SREVAERLRSHSSPFVGKQLRQLLDDAGFVRAEASASVLNHGTADETRAVGQFTADLF
                     RHPASVERAVREGIATRDELEEMARAWTEWGERRGAFLARFWCEAVAWAP"
     gene            complement(7986..8972)
                     /locus_tag="VH419_13275"
     CDS             complement(7986..8972)
                     /locus_tag="VH419_13275"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY7044635.1"
                     /translation="MRILFIGGTRFVGRAMAEAAIERGHDVTLLHRGQTEDPAFEHVE
                     HLRADRNDDLGVLSGREFDATVDVCAYVPRQVTRLAEALDGRGGHHVFVSTMSVFADT
                     DAPGLDEDGTLVTLDDPDTEEVTDQTYGGLKVLCEEAATAAYDAVTVVRPTYVIGPHD
                     HTGRFTWWVRRIARGGEVLAPGPHDAPMQVVDARDQGDWTIRMLEDGTTGIFNSTTPT
                     PPFGFGDLLDAAVRAVGPHGTRLVWVEPGWLGERGETYQSLPLWTEGSPEWTMAADPT
                     KAIAAGLNPRPLTETIADTWTWIKAAQPPLVPGWGIPEEREAELLAEWGEAS"
     gene            9642..10835
                     /locus_tag="VH419_13280"
     CDS             9642..10835
                     /locus_tag="VH419_13280"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat domain-containing M23 family
                     metallopeptidase"
                     /protein_id="HEY7044636.1"
                     /translation="MRNRTGVLAAIATPAVTMLLGLCGALPASAISKPTFQMPFACGE
                     RWEASSRPTHSPSPWAVDWNRDADDLGHIVVATAPGVVTSVTNLGDKSYGLYVVIDHG
                     GGWTTLHAHLLRAYVVLGEHVDQGQVIALLGSSGGATGPHLHYEQRLNRSDRHAAFNG
                     RRLRYDSWLRSRNCGDFPVTGDWNGDRRSDVGVFGRRAVSAAFRERLPDGTAIATSFG
                     APTDQPVVGDWNGDGQSDLGAFSPSTARFRLQKGSAGRTSFTFGKPGDLPVTGDWDGD
                     GRWDVGVFRPPTHTFYLRDALGNVSSRVFGTVSSLPVVGDWNGDGRFDVGVYDPATST
                     FVLAMPDNTVLRVKYGTATSLPVTGYWNRDPITDLGVWDRTTGTFLERVRPHKTTTIA
                     FGRRR"
     gene            complement(10846..11739)
                     /locus_tag="VH419_13285"
     CDS             complement(10846..11739)
                     /locus_tag="VH419_13285"
                     /inference="COORDINATES: protein motif:HMM:NF013722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HEY7044637.1"
                     /translation="MPDVPVRVLAKSPPLPLLYVRTALSAVPGASLLMGRGHRPRNGD
                     VPDLRLELHDVTVDADHLAAYREVCRYPPRPGLPATYLHVLAFPLHLVLMTKGGFPFA
                     PMGLVHVANRIEQVRPIGIDESLDLAVWAEDIHPHTRGRTVTVVSEGSVRGEVVWRDE
                     TVLLKRGPGDPDAPDVQEPLPSEASPSGPARWPLAGNLSRRYAAVSGDRNPIHLYDLT
                     AKPFGFRRHIAHGMWTKARCLAALDNRLSDAFRVEVAFRKPIELPGTALFGARQLPGR
                     LDFDVHGTSGSTHLAGCVTTS"
     gene            complement(11732..13069)
                     /locus_tag="VH419_13290"
     CDS             complement(11732..13069)
                     /locus_tag="VH419_13290"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEY7044638.1"
                     /translation="MADRYQAFTETPVGKLAVKNLGLPKPVRLERYTEGSPWLTGEFL
                     VGGDATAVAMATHAITAAGGTVTAERAGGVRYQGLVFDASAFTSPDDLALLPAFFTPV
                     LRSLARCPHVVVIGRPPESTTSPPARIAHRALEGFTRSLGKEIGRGGTVQLVYVEPGA
                     EAGLTSTVQFLLSPKSAYVSGQVVQVGNDVGTDQSPVDPLRPLPDRVALVTGASRGIG
                     EAIARTLHRDGAVLVGVDVAQAADDLHRVMGSLGGQAVILDITAVDAPQRIARMLAEH
                     HDGVDVLVHNAGITRDKKLANMTEDRWRQVVDVNVAAPSRITTELLDQKLIRPNGRVV
                     GVASIAGIAGNPGQTNYAASKAGVIGFVDAFAPLAAGQQVTVNAVAPGFIETQMTASI
                     PLLIREAGRRMNSLSQAGLPVDVAETVSWLAHPGSAGVTGNVVRVCGQSLLGA"
BASE COUNT         1986 a         4477 c         4555 g         2124 t
ORIGIN      
        1 ggtgacgatc tgcggtgcgg gcgtgttggc agtcacctga cccacggtac gaccgccggc
       61 aaacgcccgc ggaatgcggg aggttccgcg atttcgacgg cagaatcgcg gaactccccg
      121 caattcgcgg ggaggctcag ccggtgaggc gaaggacgtt ccaggagagg gtggggagga
      181 cggcgtcgag gtgatcgctg tcaagacgta cgtcggccaa cggcctgggt atcacccggt
      241 ccgggtcggc ggcaacgttg cgcgcatgcg ggtcgtcgtg cgtcagcgtc gtggcctcga
      301 cccgatccag accgtcgaac gcacggaggt ccaggtccag cttgatcggc tcgctccggc
      361 tgcggttgac ggcgaacacg acgagaccac ccgggccgcc gggctcgccg tcgtacgtcg
      421 caaccgcgtc gagcagcggc acctctccgt gcttcgacgt ctcctggacg gggcagcgcg
      481 tctggacccg cagcacgtca ccgctggcgt agcgcgtcac ctgcgcgaac gggtggaacg
      541 tcgtctgtcg ccacgcactg ccacccgcct cggtgaggat cggcgcgatg acgttgacca
      601 gctgcgcctg gcaggcgatc ccgacccggt cgacgtgccg cagcaaggcg atgagcaacg
      661 agccgacgac cacggcgtcg accatgtcgt aggagtcctc gatcaggctc ctggactctg
      721 cccagtcgag gccacccgac gggaagttct gccggtacca gacgttccac tcgtcgacag
      781 acaaccgcag ccgtttcgga gagccgacct ccgcgcggac acggtcggcg acggtcacca
      841 cggcatcgat ggcccgctcg aggtgcaccg acgatgccag gaagctgtcg aggtcgtcgc
      901 cttccggctc gtagtaggca tgcaacgaga cggagtccac caggtcgtag cactcgccga
      961 gaaccgtttc ctcccacgaa ccgaacgtcg gcatctctgc gtatgagctt ccgcaggcca
     1021 ccagctcaac ggacgggtcg accatccgca tggcacgcgc ggtctcggcg gcgagtcgcc
     1081 cgtactcgtg ggcggtcttg tgcccgacct gccagtcgcc gtccagctcg ttgccgaggc
     1141 accacagctt gacggcgtac ggctcggcgc ggccatgtga acgccgcagg tcggaccaca
     1201 tcgtgccgcg agggacgttg cagtactcca ccaggtcgac ggcagattcg actccgcggg
     1261 tgccgaggtt cagcgccatc atcggctctg ttcccactgc tgcacaccac gccatgaact
     1321 cgtcgacacc gaacgcgttc gactcgatcg tccgccatgc caggtcgagc cggcgtgggc
     1381 gctgctgtgg cggtccgatg ccgtcctccc agcgatagct cgacacgaag ttgccgccgg
     1441 ggtagcgcac caccgtcgga ccgagttcgc ggaccaggtc gagcacgtcc cggcggaacc
     1501 cgtcagcatc ggcgcgagga tgtcccggct cgaagatgcc ggtgtagacg caccttccca
     1561 tgtgctcgac gaacgacccg aacagccgct gctcgacggg accgacacgg agggcgggct
     1621 cggtcgtggc ggtggccgtc tccatgaagg tgctcccttc gccgatgagg tacgccgcac
     1681 cctctcacag gatcaaccgg tgacaaccac acccatccgg taccagtccg acgacgagga
     1741 cagtgcgcgg tgacgggacg acacgggact accggcccat gacgccggga cacgtcccca
     1801 ggcaaccttc caatcgaggc cagagcctgt ctggccaggt gagggaggaa ccagatgtcc
     1861 agcacagttc gcgaccggtg cgccgcggcg accggggcgc tctacgtcgt actcgtcatc
     1921 gtcggcaacg agctctacac ccgagggacg catcagtcag ctcatccgtc cggagaccag
     1981 gtgctcgccg acgcccggca catcgccagc agcagcatgg cccatgtcgg cttcgcgatg
     2041 gagggactgg gcttcgtggc gctcgtcgcg ttcctcggct acctgggcga cgtgctcatc
     2101 cgccgccgca gggagcaggt cgccagcaac gtcgccgccg tcatcgcgat ggtggcaggc
     2161 atcacaggag tggcgatcaa gctcggttcg gctgtcccga cggcggcgct gatgttcgac
     2221 cacaagacgc tcagccctga gctggcccag ctgctcaacg acatgagcac cgtcggttgg
     2281 gtgctcagct ggctgccgtt cgtgatcttc gtcgccgccg gcggctgggc actccaccgc
     2341 tgctccctgg tcgggaagcc cacggcgtac gtcgctctgg tgatcggtgt ggtaggcatc
     2401 ccgttggcgg tgctgggact cgaagacctg gccaacctga acccgatcgc gttcttgctc
     2461 ggcttgctgt ggacgttggt ggtcagcatt cggctggctg tccggccagg tgtcgtgcgc
     2521 gcgtcggaac cgtacgacgc caccgtctcg accggcatgc cagtcggagc ggcgaactga
     2581 gcgcaaacga ggacagcagg cacaggtcgc gggacgatac agcggtgatt cggtggggca
     2641 tcgccaagga tcccgcggcc tgggcctggg tcgcggtggt cgcagccatc gtcgacacgg
     2701 tcgcgctggg cctgatgggc gtggtgatcg cggtcgagga catccagacg cggatcgtcc
     2761 aggacggcgc cttcatcaac ctggtggccg gcccgacctt cccgctgctc gctgcactga
     2821 tgctgcggca cagcgaccgg gacacaccgc gtcgacagga ccgcctcgca tggctcttcc
     2881 tcggtctcgg tgtcctctgc accgccaccg gggtggtgtt cgtgaccgct gcctacggac
     2941 tgcgccacga cctgccgttc acccaagcag ccgtctgggt cgagggctgg ctgtggacgt
     3001 tcgtgggtcc cggagtcatc ctgctgctgc tgtggtttcc caccggcgac gtgccagggt
     3061 cccgttggcg ctgggctgtc cgaggcgtcg ttgcggccaa cgtgtgtctg tggatggcgg
     3121 cggcgttcat gcccgggtcg tacagcgact tcactgcaga cgtacacaat ccagtgggct
     3181 ggagcggggg cgagccgctg cttcaggttc tcggagggat cggacaggca ctgctcgccg
     3241 ccaccttctt cgcggcgctg gcgtcgctgg ccgtgcgcta ccggcgaggt gacgaacgtg
     3301 tgcgttcgca gctgcggtgg ctgctcactg ccgtcgtcgt catcgccgtc accatcgctg
     3361 tgccgacacc cgagtcgatc gaccccgtgg tcttggtgct caacgtcttg gcgacagcgt
     3421 tgttgccatt gacactcgcg gtcgcactca cgcgtggcgg ctacgccctg ccgcgcgtcc
     3481 tggtgtacgg catgctgtcg atcctgctgc tcatcgcgta cctcgcggtc atcggtgtcg
     3541 cggacgctgc gttcggcagc ggggccgatc gggctgcgac gttggcggcg gctggcctga
     3601 tcgccgtcct ggtcgcgccg ttgcgggccc gcttgcagca gtcggtggac cggctcgtgt
     3661 acggcggccg gggggatcct tacgaggcgc tgaccgactt cggccggcgg atcgccggga
     3721 gcccggacga cctactggca caggtggtcc gggtggtatc ggaggccttg cgggcgccat
     3781 acgtcgcggt cgagctgccg ggcgacatca cgccgacgac gtcggtgggg aacgcggtgg
     3841 aggattcggt cgcgatgccg ctctccctgc gtgggcaaaa cgtcggacgg ctcgtcgtcg
     3901 gccgacgcga ccgacgcgaa cagtacgggc gacgcgacat gctgctgctc gaggacctgg
     3961 cgcgccatat cgccgtcgcc gcacacgcgg cggtcctcac caaagacctg caacggtctc
     4021 gcgagtcgct cgtggtggcg cgcgaggagg agcggcgtcg gatccgacgg gacctgcacg
     4081 atggtctcgg gcccgcgctc gccggcgttg cgttcggcct ggacgcggcc aggaacacgc
     4141 tgcgggtgga cctgacgtcg accgaccgtg cgcttgccga gctcaagacc gagctgcagg
     4201 cgtcgatcgc ggacgtgcgt cggctcgtct acgacctgcg tccgcccgcc ctcgaccagc
     4261 tcggcctcgt tcctgccctg gaggagtacg ccgcccgact ccgtgagcgc gccaccctgg
     4321 acatctccgt gacctcgccc gcgctaccgg ccctgccggc ggcggtggag gtggcggcgt
     4381 accggatcgc caccgaagcg ctcaccaacg tcgcccggca ctcgcgggcg cgctggacga
     4441 ccgtcgcgtt cgcgctcgag gacagctgcc ttcgattgca ggtgctcgac aacggcgtcg
     4501 gggtcgcgtc ggccaacggt tccaccggcg tcggcttggc ggccatggcg gagcgggcgg
     4561 ccgagctcgg cgggtcctgc cgggtgaccg cccgctcggg cggtggcacg tcggtcgtcg
     4621 ccgagctccc gctgctggtg gcgctgtgag cacccgcgtg ctgctggtcg acgatcatcc
     4681 gaccttccgt cgtgggctgg gtgcgctgct cgagtcgctg cccgacatcg aggtggtggc
     4741 tgaggcagga gacggtgagg ccgcgatcgc gttggccaca tccctgcagc cggacgtggt
     4801 catgatggat ctcaacatgc ctgagctcaa cggcgtggag gcgacccgcc ggctcgtggc
     4861 ggccaacccc gacgtcgccg tgctcgtact gaccatgctg gacgacgacg aggcggtctt
     4921 cggtgcgatg cgcgcgggtg cccgtggcta catcgtcaag ggggcggaca ccgacgacgt
     4981 cgtacgcgcg ctcgagtcgg tggctcgcgg cgacgcggta ttcggtccgg ccgtcgcgtc
     5041 ccgggtgctc agctacctga ctcggccgct gtccgctcgc gacccgatgc tgttcccgga
     5101 gctctccgag cgcgaacgtg aggtgctcga gctgatggcc cgcgggctga cgaactcaga
     5161 gatcgcccgg aggctcgtcg tgtcaccgaa gacggtccgc aaccacgcct ccaacgtgtt
     5221 caccaaactg caggtctcgg accgcagcga ggcgatcctg cgcgcccgca acgccggcct
     5281 cggcagctga cgtcccgcga attgcgggaa gttcctggat tccgaccgac gaaatcgcgg
     5341 aactcccctc aattcgcggg ctcgtaggat caaccggtga cagtcacacc catccggtac
     5401 gtgtccgacg acgaggacag tgcgcggtgg ttgtcgttcc cgttccggga cggtgacatc
     5461 gtcatctcga cccggtccaa gagcgggacc acgtggatgc agatgatctg cgccctgctc
     5521 gtgttccagt cgccggacct gcccgagccg ctcgccaccc tctcgccctg gttggactgg
     5581 aagatcgcac cgatcggtga ggtgatcggt cggctcgagg cgcagcagca tcgtcgtttc
     5641 gtgaagacgc acacgccgtt ggacggcgtt ccgcaggacg gcagagcgac gtacatcgtg
     5701 gtcgcccgcg acccgcggga catgtacgtc tccctctacc atcaaggcga caacatcgac
     5761 cgggacgcag tacgccgcct gctcggacag ccagagcggg ccccggacga cgtacgaccc
     5821 gaacggccgc cgctcgacca ggcactggag gcgtggatcg gccgggacga ctcgccgcag
     5881 cagagcatgg actcgatccg cggtgtcttc catcacgtca cggatgcgtg gcgacgacgc
     5941 gagacgcagt cgaacgtcct cctcgtgcac tacgacgatc tgctgcacga tctcgacggc
     6001 cagatgagag acctggcggc ccggttgggg ctcgacgtcg ccgagaagcg atggccggag
     6061 ctggtcgacg ccgcgacgtt cacgacgatg cgggaacgcg ccagcgccgt cgtcccggac
     6121 acggcgggcg tgctgaagga tgcgaacgcg ttcttccgtc gtggctcgtc cggcgcctgg
     6181 cgcgacgtga tgaccgcagg gcaggtggag cactactggc gacgggtcga cggtctcgcg
     6241 ccgccggagg tcgtggcctg gctgcaccgc tgaccgggtg acatgatcgg ctggtgccga
     6301 ccaccgtcgc ggacctgctc gatctgctgg agctcgaaca gctcgaggag gacctctatc
     6361 gcggtcgcca gccggatacg gccatgcagc gggtgttcgg cggccaggtg gcggggcagg
     6421 cgctcagggc tgcctggcac acgatcggcg cagggctcgc ggtgcattcg ctgcactcgt
     6481 acttcctacg tcccggcgac acgtccgtgc cgatcatcta cgacgtcgaa cggatccgcg
     6541 acggtcgttc gttcgccacc aggcgggtcg tggcccgcca gcacgggaag tcgatcttct
     6601 acctgaccgc gtccttccac atcgaggagc ccggtttcga ccaccaggac gccatgccgg
     6661 acgtgcccga cccggaggac tgtctgtcga tgggtgacct gttccgccgt atcaccggac
     6721 gtgacgacga cacgtcctgg gaacgagagt gggccgcgct cgacgtgcgg tacgccgccg
     6781 actcccggcc cggcggtgcc ctgcagaacg cgcagcagcc tgctcgtaca cagatctggg
     6841 tcagggcggc cgacaaggtc cccgacgagc cggtcatcga cacctgcgtg ctcacctacg
     6901 tcagcgacct gacgctgctc ggtgtgagcc tggtcccgca cggcgtgatc atcgcgtcgc
     6961 ggcagatcca gcccgcatcg ctcgaccact cgatgtggtt ccaccggccg ttccgctccg
     7021 acgagtggat gttgtacgac caacactcgc cctcggccac cggcgcccgc ggcttcgccc
     7081 tgggaacggt gttcagccgg gacggcgtcc tcgtggcctc ggtcgctcag gagggcctga
     7141 tccggccgat cggcgacgcg cgggaccggc tgctgcgctc cgctgattga tccgcggtca
     7201 cggcgcccag gcaaccgcct cgcaccagaa ccgggccagg aaggcgccgc gccgctcgcc
     7261 ccactcggtc caggcgcggg ccatctcctc gagctcgtcg cgagtggcga ttccctctcg
     7321 gaccgcgcgc tcgaccgagg ccggatgtcg gaagaggtcc gcggtgaact gtccgaccgc
     7381 tcgcgtctcg tcggcggttc cgtggttcag cacggatgcg gaggcctcgg cccgcacgaa
     7441 accagcgtcg tcgagcagct gccggagctg tttgccgacg aacgggctgg agtgggagcg
     7501 gagccgctcg gcgacctccc tcgaacgccg gagaacggcg ttcgtcgggt gaagcagctc
     7561 gccgtcccag tcagcatcga ccagtgcgat gacggctcct gtgcgggcga cccggcgcgc
     7621 ctctcgcagg acgtccgcgg gctcggccac gtgctggagc actgcgctgg cgaagatcgc
     7681 gtcgaagctc gcgtcgggga acggcagacg tcgtacatcg ccgacgacca ccgcgacgcc
     7741 gttgaccggt acggcgtcca cgtcgacacc gacggtgagc cctggtgtga cagcggcggc
     7801 gaggccgacc gtgatcgaac caggcccgca gccgaggtcg atcagccgca tcccgggccg
     7861 caggtgcgga agcaggtgcg cagcgtggct ctgcgccgtc cgctgggcgt gcgcgccgcg
     7921 atgcggacgc atccgctcgc tcgtcgcggc tgcctggctc tcgtcgtgat cgtccgggtc
     7981 gacgctcagg atgcctcgcc ccattcggcg agcagttctg cctctcgctc ctccgggatc
     8041 ccccagcccg gaaccagcgg cggctgcgcg gccttgatcc acgtccaggt gtccgcgatc
     8101 gtctcagtca acggtcgagg gttgagcccg gcggcgatcg ccttcgtcgg gtccgcggcc
     8161 atcgtccact cggggctccc ctcggtccac agcggcagag actgataggt ctcgccgcgt
     8221 tccccgagcc agccaggctc gacccagacg agccgcgtgc catgcggacc cactgctctg
     8281 acagcagcgt cgagcaggtc accgaagccg aacggaggcg tgggtgtcgt gctgttgaag
     8341 atcccggtcg tcccgtcctc gagcatcctg atcgtccagt cgccctggtc ccgtgcgtcg
     8401 acgacctgca tcggcgcatc atgcggccca ggtgccagca cctcaccgcc gcgcgcgatc
     8461 cggcgtaccc accaggtgaa ccgcccggtg tgatcgtgcg ggccgatgac gtacgtcggc
     8521 cggaccacgg tcacggcgtc gtacgccgcc gtcgcagcct cttcacacag caccttcagt
     8581 ccgccgtagg tctgatcggt gacctcctcg gtgtccgggt cgtcgagcgt gacgagcgtg
     8641 ccgtcctcgt cgagccccgg cgcgtcggtg tcggcgaaga cggacatggt cgacacgaac
     8701 acgtggtggc caccgcgacc gtccagcgcc tccgcgagcc tggtgacctg gcgtggcaca
     8761 tacgcacaga cgtcaacggt ggcgtcgaac tcgcgaccgg acagcacgcc gaggtcgtcg
     8821 ttgcggtcgg cgcgcaggtg ttcgacgtgc tcgaaggcag gatcctcggt ctggccgcgg
     8881 tggagcaggg tgacgtcgtg accgcgttcg atcgcggctt cggccatggc ccgtccgacg
     8941 aaccgggtcc cgccgatgaa gaggatgcgc atggctcgac cctagggtgc agcctgttcc
     9001 cgcgcacacc gcaatgcatg ccaaccaacg ccgtgcccgc cagacctcgg ccggctacgg
     9061 cccttccgtg ggccgcgctc gccaccagtg cggccgtggt ggtttgcctg ggtagccatc
     9121 cggatggacg ggtagccagg caggtctgcc ttgggaggga tggctttgcg tggtgaccca
     9181 ggcaactctt gcaggcgcct caggtcgtcg tggcggaaat ggtcgcgatg ggcgtgcgtg
     9241 gtcatcggct gtctcctgtc atggggctga ggtaagtcgg cagcgaagag cggaaacgtt
     9301 cgcgccgact gagctggtcg gccgacgttc cgttcctttt cgttggggtg tgactggcgg
     9361 ttttcggacg tttgcgctga ccggcctgat gtttgcgatg tcaacatcca agacccctgt
     9421 gcccatcgga ggtttgcgat gtttacacgc cttttgggct gtaaacatcc caaacctcga
     9481 cgcgtgaggg cgtgcacctg ggaggcgcgg tgcgttgagg cctgaggagt actcggagtc
     9541 ggtggcaatg agccggaaag ccttgacctg cctgggacgc gagtgactag tgtgccggtt
     9601 gggaaaacag ttccccggac tggccgggag gtggtcggct cgtgcgaaac cgcaccggtg
     9661 tcctggcagc catcgcgacg cctgccgtca cgatgctcct cgggttgtgc ggcgcgcttc
     9721 cggcatcggc catctccaag ccgacattcc agatgccgtt cgcctgcggt gagcgctggg
     9781 aggcgtcgag ccggccgacg cacagcccaa gcccctgggc agtggactgg aaccgagacg
     9841 ccgacgacct cggccacatc gttgtggcca ccgcgcccgg cgtcgtcacg tcggtcacca
     9901 acctcggcga caagagctac ggcctctatg tggtcatcga ccacggcggc ggctggacga
     9961 cattgcacgc gcacctgctc cgtgcgtacg tcgtcctcgg tgaacacgtc gatcagggcc
    10021 aggtcatcgc gctgctcggc agctccggag gtgcgaccgg gccgcacctg cactacgagc
    10081 aacgactcaa caggtccgat cggcacgccg cgttcaacgg acggaggctt cgctacgaca
    10141 gctggctcag gtccaggaac tgcggggact tcccggtgac cggtgactgg aacggtgacc
    10201 gccgtagcga cgtcggcgtg ttcggtcggc gggccgtgtc ggccgccttt cgtgagcgac
    10261 tgcccgacgg tacggcgatc gcgacatcgt tcggcgcgcc tacggaccag ccggtcgtcg
    10321 gcgactggaa cggcgacggt cagtccgacc tcggcgcgtt cagcccgtcg acggcccgct
    10381 tcaggctgca gaagggcagc gcgggtagga cgtcgttcac cttcggaaag ccgggcgacc
    10441 tgccggtgac gggcgactgg gacggggacg gccgttggga cgtcggcgtg ttcagaccgc
    10501 ccacacacac gttctatctc cgcgacgcgc tcggcaacgt ctcgtcacgg gtgttcggta
    10561 ccgtgtcgag cctgccggtc gtgggcgact ggaacgggga cggacgcttc gacgtcggcg
    10621 tgtacgaccc ggcaacgtcg accttcgtgc tcgcgatgcc cgacaacacg gtgctgaggg
    10681 tcaagtacgg caccgccacc tcactcccgg tgaccggcta ctggaaccgg gatccgatca
    10741 ccgacctcgg tgtgtgggac aggaccacgg gaaccttcct cgaacgcgtt cgcccccaca
    10801 agacgacaac gatcgcgttc ggccggcgtc gctgagacca gccggtcagc tcgtcgtgac
    10861 gcatccggcc aggtgggtcg aacccgatgt gccgtgtacg tcgaagtcga gccggcccgg
    10921 cagctgacgc gcaccgaaga gtgccgtccc cggcagctcg atcggcttgc ggaaggccac
    10981 ctcgacgcgg aaagcgtcgg acaacctgtt gtcgagcgcg gccaggcagc gcgccttcgt
    11041 ccacatgccg tgcgcgatgt ggcggcggaa cccgaacggt ttggcggtca gatcgtagag
    11101 atggatcggg ttgcggtcgc cggacaccgc ggcgtaccga cggctgaggt tcccggccag
    11161 cggccacctc gccggcccgc tgggcgacgc ctcgctcggc aacggctcct gcacatccgg
    11221 tgcatccggg tcgcccggcc cacgcttcag caacaccgtc tcgtcccgcc agacgacctc
    11281 gcccctgacg ctgccttcgg aaacgacggt gaccgtgcga ccccgtgtat gcgggtgtat
    11341 gtcctcggcc cagaccgcca gatccagtga ctcgtcgatg ccgatcggcc ggacctgctc
    11401 gatccggttg gcgacgtgca cgaggcccat tggcgcgaac gggaagcccc ccttggtcat
    11461 cagcaccagg tggaggggga acgccaacac gtgcaggtac gtcgccggca gaccaggtcg
    11521 cggcgggtac cggcacacct cgcggtacgc cgcaaggtgg tccgcgtcca cggtgacgtc
    11581 gtgcagctcg agccggaggt ccggtacatc gccgtttcgc ggccggtgtc cgcgtcccat
    11641 gagcaacgac gcaccgggta ccgcggacag cgcagttctc acgtagagca gcggcagcgg
    11701 cggcgacttg gcgagcacgc ggaccggcac gtcaggcacc gagcaggctc tggccgcaga
    11761 cgcgtacgac gttgccggtc acgcctgcgg agcccgggtg cgccagccag gagacggtct
    11821 cggccacgtc gaccgggagg ccggcctggc tcagcgagtt catccgacga cctgcctccc
    11881 ggatcagcag cgggatcgac gcggtcatct gggtctcgat gaagcccggt gccaccgcgt
    11941 tgaccgtgac ctgttgtcct gccgcgagcg gcgcgaacgc gtccacgaac ccgatgacgc
    12001 cggccttcga agcggcgtag ttggtctgcc cggggttgcc cgcgatcccg gcgatcgacg
    12061 cgacaccgac gacgcgaccg ttgggacgga tcagcttctg gtcgaggagc tcggtggtga
    12121 tccgcgaggg cgcggcgacg ttgacgtcga cgacctgtcg ccagcgatcc tcggtcatgt
    12181 tggcgagctt cttgtcccgc gtgatgccgg cgttgtgcac gagcacgtcg acgccgtcat
    12241 gatgctcggc aagcatccgg gcgatgcgct gcggagcgtc cacagcagtg atgtcgagga
    12301 tcactgcctg accgccgagc gaccccatca cgcggtgcag gtcgtcggcc gcctgggcca
    12361 cgtcgacgcc gaccagcacg gcgccgtcgc ggtgaagcgt tcgtgcgatg gcctcgccga
    12421 tcccacgcga tgctccagtc accagcgcaa cccggtcggg gagcggcctg agtgggtcaa
    12481 cgggcgactg gtcggtgccg acgtcgttac cgacctggac gacctgtccc gagacgtacg
    12541 ccgacttcgg cgagagcagg aactggacgg tggaggtcag cccggcttcg gcgcccggct
    12601 cgacgtacac gagctggact gttccgccgc ggccgatctc cttgccgagc gagcgggtga
    12661 agccctccag tgcgcggtgc gcgatccgcg ccggcgggga cgtcgtcgac tccggcggtc
    12721 tcccgatgac caccacgtgc ggacatcgag cgagcgaccg cagaaccggc gtgaagaacg
    12781 ccggcagcag ggccaggtcg tcgggactgg tgaaggcgct cgcgtcgaac acgaggccct
    12841 ggtaccggac gccgccggcc cgttcggcag tgaccgttcc gcccgccgcg gtgatcgcgt
    12901 gcgttgccat ggcgacagcg gttgcgtcgc ctccgacgag gaactcaccg gtcaaccacg
    12961 ggctgccctc cgtgtagcgc tccagccgga ccggcttggg caggccgagg ttcttgacgg
    13021 ccagtttgcc gaccggtgtc tcggtgaagg cctggtagcg gtctgccacg ggtacctact
    13081 cctgtctggt gagccggtgt cgtgaaacgg acgcacccgt tgcacgtaag gcttgagatg
    13141 ta
//
LOCUS       DATBFZ010000435         2582 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_5402663, whole genome shotgun sequence.
ACCESSION   DATBFZ010000435 DATBFZ010000000
VERSION     DATBFZ010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2582
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5402663"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1776)
                     /gene="pknB"
                     /locus_tag="VH419_13295"
     CDS             complement(<1..1776)
                     /gene="pknB"
                     /locus_tag="VH419_13295"
                     /inference="COORDINATES: protein motif:HMM:NF033483.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEY7044639.1"
                     /translation="MTQPRLVGARYELGELIGYGGMAEVHRGRDIRLGRDVAIKVLRA
                     DLARDPSFLNRFRREAQSAAGLNHPSIVAVYDTGEDVGAEGTSQPFIVMEYVEGRTLR
                     DILKAEGKLPPRRAMEIVADVAAALDFSHRSGLVHRDVKPANVMITQTGAVKVMDFGI
                     ARAVADNSATVTQTANVIGTAQYLSPEQARGEAVDARSDVYSTGCLLYELVTGVPPFQ
                     GDSPVAVAYQHVRENATAPSSRVPGLPRAMDSIVMKALAKNPQNRYQSAGEMRSDLQR
                     ALADQPVSAESVMTDAERTQFIARTPPPPIPMPRHEAAGEPEPERRNGVIWAAIVVAL
                     LLVVGTGAFFIWKLGSSDDATPKVTVPSLVGLSVEQANQKLGNTPNCDQDSSCKVYLQ
                     PDPNTPTTPGPCDDGTTPKQDGGICKVLQGDNTVAAGSQVEEGTTILYETYKKAVVIV
                     PYVIGSSYADAQSVIERAGLHIKKGKDVNTFGQDKGDVVSQDPDNNTSVNPGSDVTVR
                     LSTGKAKLPDVTGMKADEAKSKLNGMGFTSVDNSQTKVTHDQKKGGLVATQDPTPGNA
                     YTADVHITLTIWKYVKPAPTCDPNKT"
     gene            complement(1868..>2582)
                     /locus_tag="VH419_13300"
     CDS             complement(1868..>2582)
                     /locus_tag="VH419_13300"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEY7044640.1"
                     /translation="KTASLEFAFAKSCNTLFSALAVEQLGGQAIADEAKLFGFDPDDQ
                     LEIPLPVAKSTVGNTDDLTAKGFLARTAFGQQDVRMTTLQGAMIASAVANNGILMHPY
                     LVDRELAPDLSELRTTQPSQLSQVLEPTQDQQLQAMMEGVVTAPEGTGHSAAIDGAVV
                     GGKTGTADTGRKFKNGQDEPPDAWFIGYAMTNGSPKIAVAVLIENGGVQGNETTGGEA
                     AAPVAKAVMEAYLKGHGGS"
BASE COUNT          325 a          868 c          893 g          496 t
ORIGIN      
        1 cgtcttgttc gggtcacacg tcggcgccgg cttcacgtac ttccagatcg tcagcgtgat
       61 gtgcacgtcg gcggtgtacg cgttgcccgg agtcgggtcc tgcgtggcga ccaggccgcc
      121 cttcttctgg tcgtgcgtca ccttggtctg cgagttgtcg acgctggtga agcccatgcc
      181 gttgagcttg gacttggcct cgtccgcctt catcccggtg acgtcgggca gcttggcctt
      241 gcccgtcgac agccgcacgg tcacgtccga gccgggattg accgaggtgt tgttgtccgg
      301 gtcctgcgag accacgtcgc ccttgtcctg gccgaacgtg ttgacgtcct tgcccttctt
      361 gatgtgcagc cccgcccgct cgatcacgga ctgtgcgtcg gcgtaggacg acccgatgac
      421 gtagggaacg atgacgaccg ccttcttgta cgtctcgtac aggatcgtcg tgccctcctc
      481 gacctggctg cccgccgcga ccgtgttgtc gccctgcagc accttgcaga tcccgccgtc
      541 ctgtttcggc gtcgtgccgt cgtcacaggg ccccggcgtc gtcggagtgt tcgggtccgg
      601 ctgcaggtac accttgcacg aactgtcctg gtcgcagttc ggggtgttgc cgagcttctg
      661 gttggcctgc tcgaccgaca gcccgaccag gctcggcacc gtgaccttcg gcgtcgcgtc
      721 gtcggagctg cccagtttcc agatgaagaa cgcgccggtg ccgacgacga gcagcaacgc
      781 caccacgatc gccgcccaga tgacaccgtt gcggcgctcc ggttcgggct cgcccgccgc
      841 ctcgtggcgc ggcatcggga tcggcggcgg tggggtgcgc gcgatgaact gggtgcgctc
      901 ggcgtcggtc atcaccgact ccgccgagac cggctggtcg gcgagcgcgc gctgcaggtc
      961 gctgcgcatc tcgccggcgc tctggtagcg attctgcggg ttcttggcca gcgccttcat
     1021 cacgatcgag tccatcgccc gcggcagccc gggcacgcgg gacgacggcg ccgtggcgtt
     1081 ctcgcgcacg tgctggtagg cgaccgcgac cggggaatcg ccctggaacg gcggcacgcc
     1141 ggtcaccagc tcgtagagca ggcacccggt cgagtagaca tccgagcggg cgtcgacggc
     1201 ctcgccgcgc gcctgctccg gcgagaggta ctgcgcggtg ccgatgacgt tcgcggtctg
     1261 ggtgaccgtg gccgagttgt cggcgacggc acgggcgatg ccgaagtcca tcaccttgac
     1321 cgcgccggtc tgcgtgatca tcacgttggc cggcttgacg tcacggtgca ccagcccgct
     1381 gcggtggctg aagtccaggg cggcggccac gtcggcgacg atctccatgg cccggcgtgg
     1441 cggcagcttg ccctcggcct tgaggatgtc gcgcagcgtg cgtccttcga cgtactccat
     1501 gacgatgaac ggctgcgagg tgccttccgc gccgacgtcc tcgccggtgt cgtagaccgc
     1561 gacgatggac gggtggttga gtccggccgc ggactgggcc tcacggcgga accggttcag
     1621 gaacgacggg tcgcgggcca ggtcggcgcg cagcaccttg atggcgacgt cgcggccgag
     1681 ccggatgtcg cgaccgcggt gcacctcggc cataccgccg tagccgatga gctcgccgag
     1741 ttcgtagcgc gcgccgacca gtcgaggctg ggtcatctct gtcgtgtcct tactgtcctg
     1801 tgcgaccgtc ctggcgctgc ttccatcagt gtcccggctg cgagctgatc ttggtgtcgt
     1861 tgtgtcatca gctaccgccg tgtcctttca ggtacgcctc catgaccgcc ttggcgaccg
     1921 gagcggccgc ttcaccgccg gtcgtctcgt tgccctgcac gccaccgttc tcgatgagga
     1981 cggcgacggc gatcttcggg ctgccgttgg tcatggcgta tccgatgaac caggcgtccg
     2041 ggggttcgtc ctggccgttc ttgaacttgc gtccggtgtc ggcggtgccg gtcttgccgc
     2101 cgacgaccgc gccgtcgatg gcggccgagt gaccggtgcc ctcgggcgcg gtgacgacac
     2161 cttccatcat cgcctgcaac tgctggtcct gggtcggctc gagcacctgg ctgagctggc
     2221 tcggctgcgt ggtgcgcaac tcgctcaggt cgggggcgag ctcgcggtca acgagatacg
     2281 ggtgcatcag gatgccgttg ttggccaccg cgctcgcgat catcgctccc tgcaacgtcg
     2341 tcatccgcac gtcctgctgg ccgaacgcgg tgcgggccag gaagcccttc gcggtcaggt
     2401 cgtcggtatt gcccaccgtc gacttcgcga cggggagtgg tatctcgagc tggtcgtcgg
     2461 gatcgaagcc gaacagcttg gcctcgtccg cgatggcctg gccgccgagc tgctcgaccg
     2521 cgagcgccga gaacagcgtg ttgcacgact tggcgaacgc gaattcgagc gaggccgtct
     2581 tg
//
LOCUS       DATBFZ010000436         2598 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_301017, whole genome shotgun sequence.
ACCESSION   DATBFZ010000436 DATBFZ010000000
VERSION     DATBFZ010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2598
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301017"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2485)
                     /locus_tag="VH419_13305"
     CDS             complement(<1..2485)
                     /locus_tag="VH419_13305"
                     /inference="COORDINATES: protein motif:HMM:NF019353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044641.1"
                     /translation="MRARCEAGIGRRYAPDQHAAVEQRLADELGVIAQLGYASYFLTV
                     ADVVDLIGEMGVRAAARGSGAGSIVNYLLGISGVDPLRYGLLMERFLSPLRQALPDID
                     IDVESARRTEVYERILERYTGDRCACVSMMDSYRVRHAIRDVGAALGMPLTEIDSIAK
                     AFPHIRAKDARAALRELPELRASGLGEERLDVLFRLVEKLDGLPRHIAVHPCGILLSD
                     ATLLDRTPVEASYLGFPMSQFDKDDVEDLGLLKLDVLGIRMQSAMAHAVEEVRRVDGL
                     DIDVDDETQAPRDDPATYTLIQSTKTLGMFQIESPGQRELVGKFAPETFDDIIIDISL
                     FRPGPVKSDMVVPFLRARQGWSEPEYLHPDLIPVLGQTGGVVVFHEQVVEMIATLTGC
                     TLAEADEARRALGDHVGKHETRTWFFPRALGKGYPLFTVQRCWQVLEAFASFGFCKAH
                     AAAFALPTFQSAWLKTHHPAAFLAGVLTHDPGMYPKRLILDDARQFGITVLPLDVNAS
                     DDVYRVEKLDDGAVVAANCGKGPAETPVEGCTFPQFAVEELGRGYGIRLSLADVKGIS
                     DAEVARIIASRPYHSLTDLWQRARPSRPTAERLVLAGALDTLYAIGSPVSVRRRGTVT
                     RRDLLLQVAELDRWSRATDKAIRAASSSQARSRARIAASRSTHLPGSTASSGPPELRE
                     SAGRNAGPGMSFPAIRGWPDLPPEFPTTARRTTHPSRRGDDPRVLAALQSQAPLPVEP
                     VTAVQLTLDLDDTPAITEASGLPEMSGAERVRAELEILGLDVSSHVVEFYAPMLRELG
                     VTWSRDLLHRRSRSELLVAGVKVATQTPPI"
BASE COUNT          425 a          888 c          898 g          387 t
ORIGIN      
        1 ggatcggtgg cgtctgggtg gcgaccttga cacctgccac cagcagctcc gaacggctcc
       61 ggcggtgcag caggtcacgc gaccaggtga cgccgagctc acgcagcatc ggcgcgtaga
      121 actcgaccac gtggctgctc acgtcgaggc cgaggatctc cagctcggcc cggacgcgtt
      181 cggcaccaga catctccggg agcccggacg cctcggtgat cgccggggtg tcgtcgaggt
      241 cgagggtgag ctggactgcg gtgaccggct cgaccggcag cggcgcctgc gactgcagcg
      301 ccgccaagac ccgtggatca tcgccacgcc gtgacggatg ggtggtacgc cgcgccgtcg
      361 tcgggaactc cggagggagg tctggccacc cgcgaattgc gggaaaactc atgcctggac
      421 cggcgtttcg acctgcactt tcccgcaatt ccgggggacc cgaggaggcg gtagagcctg
      481 gaagatgggt cgaacgggag gcggcgatcc gggctcgaga cctggcctgg gacgacgagg
      541 cggcccggat ggccttgtcg gtggcgcggc tccaccggtc cagctcggcc acctgcagca
      601 gcaggtcacg gcgggtcacc gtgccccgtc ggcgtacgga caccggcgac ccgatggcgt
      661 agagcgtgtc gagcgccccg gccagcacca gccgctccgc ggtcggccgg gacggccggg
      721 cacgttgcca caggtcggtg agcgagtggt acgggcggga cgcgatgatc cgcgccacct
      781 ccgcgtcact gatccccttc acatcggcca acgagagccg gatcccgtat ccccgcccca
      841 gctcctctac cgcgaattgc gggaaagtgc acccttcaac cggcgtttca gcaggacctt
      901 tcccgcaatt cgcggcaacc accgcaccat cgtcgagctt ttcgacacgg tagacgtcgt
      961 cggaggcgtt gacgtccagc gggagcacgg tgatcccgaa ctggcgggca tcgtcgagga
     1021 tgaggcgctt ggggtacatg cccgggtcgt gggtgagcac gccggccagg aacgccgccg
     1081 ggtggtgcgt cttcagccag gcggactgga acgtcggcag cgcgaacgcc gccgcgtgcg
     1141 ccttgcagaa cccgaacgac gcgaacgcct cgagcacctg ccagcaccgc tggacggtga
     1201 acagcggata gcccttcccg agcgcacggg ggaagaacca cgtcctggtc tcgtgcttgc
     1261 ccacatggtc gccgagggcg cgcctggcct cgtcggcctc ggccaaggtg cagccggtga
     1321 gcgtcgcgat catctcgacc acctgctcgt ggaagacgac cacgccgccg gtctggccga
     1381 ggaccgggat gagatcggga tggaggtact ccggttcgct ccagccctgc cgcgcccgca
     1441 ggaacggcac caccatgtcg ctcttgaccg gacccggccg gaacagcgag atgtcgatga
     1501 tgatgtcgtc gaacgtctcc ggcgcgaact tgccgaccag ctcacgctgt cccggcgact
     1561 cgatctggaa catgccgagc gtcttcgtgc tctggatcag cgtgtatgtc gccgggtcgt
     1621 cgcgcggcgc ctgcgtctcg tcgtccacgt cgatgtcgag accgtcgacg cggcgtacct
     1681 cctcgacggc atgcgccatc gccgactgca tccggatccc gagcacgtcg agcttgagca
     1741 gcccgaggtc ctcgacgtcg tccttgtcga actggctcat cgggaacccg agatagctcg
     1801 cctcgacggg agtgcgatcg agcagggtcg catcggacag gaggatcccg cacgggtgga
     1861 cggcgatgtg ccgcggcagc ccgtcgagct tctcgaccag ccggaacagc acgtcgagcc
     1921 gctcctcccc cagcccggag gcacgcagct cgggcagctc gcggagcgcg gcacgggcgt
     1981 ccttggcgcg gatgtgcggg aacgccttcg cgatcgagtc gatctccgtc agcggcatcc
     2041 cgagcgccgc ccccacgtcg cggatcgcgt gccggacccg ataggaatcc atcatcgaga
     2101 cgcaggcgca gcggtcgccg gtgtagcgct cgaggatgcg ttcgtagacc tcggtacgcc
     2161 gagccgactc gacgtcgatg tcgatgtcgg gcagcgcctg tcgcagtggg gacaagaagc
     2221 gctccatcag caggccgtag cgcagcgggt cgactccgga gatgccgagc aggtagttga
     2281 cgatgctgcc ggctcccgac ccgcgggcgg ccgcacggac tcccatctcg ccgatcaagt
     2341 cgacgacgtc ggcgacggtg aggaagtacg acgcgtagcc gagctgcgcg atgaccccga
     2401 gctcgtcggc gagccgctgc tcgacggcag cgtgctggtc cggcgcgtac cgacggccga
     2461 tccccgcctc gcacctcgcc cgcaacactg ccgacccgct ggttgagctt gtcgaagatc
     2521 cgctggttga gcttgtcgaa accaaggatc cctgggacac ctcgaactct ggaaagtgga
     2581 cttcgccgag cccgaggt
//
LOCUS       DATBFZ010000437         3451 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_6388859, whole genome shotgun sequence.
ACCESSION   DATBFZ010000437 DATBFZ010000000
VERSION     DATBFZ010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 3451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3451
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6388859"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(80..1327)
                     /locus_tag="VH419_13310"
     CDS             complement(80..1327)
                     /locus_tag="VH419_13310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044642.1"
                     /translation="MVRRIYLHVGTPKTGTTYLQSVLWANRDALARQGLLLPLKGVRE
                     HFYLSVTARGSAEAKANMPPAGHTAWNRMIDEVKRSRRDVLISHELFSAGSMKEAAWV
                     HAQLRPLCQELHIVVTARDMGRQVPAEWQQTVKHGRDHTLPAFYDTLRRWDPDEPTRP
                     PLKSRPPVLFWQVQDLVAVLDRWGAGLPREHIHIVTVPPRGADKRLLWSRFAQVVNVD
                     PESVDQSVTTPNESLGVAEIELVRRVNTALPADLPRMRQQKVVKGGLADTILAARAGM
                     RKFAPSAQDYAWSVKRGTQMVEALRPLGYDVVGDLDDLIPAAQPPGGASVDDVTEGEL
                     NTVAVQTIVDLLLPSDAPAARGMDADPRRARGKIGDQQRRTNQQAKTLEQARQERRGG
                     AHRLLLRLRRAGGKVKRLITRRR"
     gene            1518..2930
                     /locus_tag="VH419_13315"
     CDS             1518..2930
                     /locus_tag="VH419_13315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020386953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEY7044643.1"
                     /translation="MSDRFQPPTYPVPASDALVEFMRTGWSDDEESVEPLPVAAWAAQ
                     RRAKLAERFPGERIVIPSGPLKPRANDTDYRFRAETAHVHLTGNQTSDAVLVMDGDEA
                     TLYFRPRHAKSDDAFWRDGRYGEAWAGRRLSLTEASETFGLPCRHLYDLPEALRALGP
                     ARVLRGVDPDVDALVPPQRDSAGDQDLAVHLSEMRLVKDVWEIEQLQDAVDSTILGFE
                     DCVREWDDAVRYGERWIEGTFWRRARANGNDVGYECISAAGPHATTLHWIDNDGPVTP
                     GHLVLLDMGVENRSLYTADVTRTLPVDGTFTADQRHLYELVLAAQEAGMAAVRPGAAY
                     NEFHLAAMGVLARGLSDMGILPVSPEEALESDSGLYRRWTLHGTGHMLGLDVHDCAAA
                     RRENYNDGKLETGMVLTVEPGLYFQSSDLLVPESLRGIGIRIEDDVVVTDDGCRNLSG
                     ALPRTATDVESWMAGTVRQR"
     gene            complement(2921..3262)
                     /locus_tag="VH419_13320"
     CDS             complement(2921..3262)
                     /locus_tag="VH419_13320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEY7044644.1"
                     /translation="MARTNIPGADKLPIRMLHDRVLVSLDHEAGERRSSAGIVIPATA
                     VMGRRLAWARVVGVGANVRTVQVDDRVLFDPEDKAEVEVRGEDFILLRERDIHAVASE
                     RLEDGQTGLYL"
BASE COUNT          546 a         1185 c         1166 g          554 t
ORIGIN      
        1 ggggtgtaaa catcgcaaac ctccgaccgc tgagccggga tcacatcccg cggtgcgcgc
       61 gggctggact ccgcgacgct cagcggcgac gagtgatgag ccgcttcacc tttccgccgg
      121 ctcgacgaag ccgcaacaac aggcgatggg ccccaccccg acgctcctgg cgcgcctgct
      181 cgagtgtctt cgcttgttgg ttggtgcgtc gttgttggtc gccgatcttg ccgcgcgctc
      241 ggcgaggatc ggcgtccatg ccacgggcag caggggcgtc cgacggcagc aggagatcga
      301 cgatcgtctg gacggcgacc gtgttgagct cgccctcagt cacgtcgtcg accgacgcgc
      361 cacccggtgg ctgcgcggcc ggaatcagat cgtccaggtc gccgacgacg tcgtaaccaa
      421 gcggtcgcag cgcctcgacc atctgcgtcc cacgcttcac cgaccaggca tagtcctgcg
      481 ccgagggagc gaacttccgc attcctgcac gcgcggccag gatcgtatcg gccaggccgc
      541 ccttgaccac cttctgttgt cgcatccgcg gcaggtcggc cggcagcgcc gtgttgaccc
      601 ttcgaacgag ctcgatctcg gcgacgccga gcgactcgtt cggcgttgtc accgactggt
      661 cgacggactc gggatcgacg ttcacgacct gcgcgaaacg cgaccacagc agtcgtttgt
      721 ccgcaccgcg aggtggaacg gtgacgatgt ggatgtgctc gcgagggagt ccagcacccc
      781 aacggtccag caccgccacg agatcttgaa cctgccagaa caacacaggt ggtcgcgact
      841 tcagcggggg acgcgtgggt tcatcgggat cccaacgacg cagcgtgtcg tagaacgccg
      901 gaagggtgtg gtcgcgtccg tgcttgaccg tctgctgcca ctccgcgggc acctgccgtc
      961 ccatgtcacg cgcggtcacc acgatgtgga gctcctggca cagcggcctc agctgagcgt
     1021 gcacccacgc cgcctccttc atcgacccag cggagaacag ctcgtgactg atcaggacgt
     1081 cgcgtcgcga gcgcttgacc tcgtcgatca tccgattcca cgcagtgtgg ccggctggtg
     1141 gcatgttcgc cttggcctcg gccgagccac gcgccgtcac gctgaggtag aagtgttcgc
     1201 gcacgccctt cagcgggagc agcagaccct gccgcgcgag ggcatcccgg ttggcccaca
     1261 gcacgctctg gaggtacgtc gtcccggtct tcggcgtccc cacgtgcaga tagatccgcc
     1321 tcaccatgtt cggtggttcc tccgcttcgt cgtacgccgc atgggccgag ccgtgtcccg
     1381 ccgtgcagcg tacccagcac cgctcggtcc gggacactca gccgtacctg gataatggga
     1441 cctgatgacc ccgagcggcg tgcgacctga tcagtggacc tcgtcgcggc gcctggttct
     1501 actgctgagg agatcacgtg tctgaccggt tccagccccc tacgtatccg gttccggcca
     1561 gcgacgccct ggttgaattc atgcggaccg ggtggtccga cgacgaggag agcgtcgagc
     1621 cgctgcccgt cgcagcgtgg gcagcacaac gccgggcgaa gctcgcggag cggttcccgg
     1681 gtgagcggat cgtgatcccg tccggaccgc tgaagccgcg ggcgaacgac accgactacc
     1741 gcttccgcgc cgagaccgcc catgtccatc tcactgggaa ccagacgtcc gacgctgtgc
     1801 tcgtgatgga cggcgacgag gcgacgctct acttccggcc acgtcacgcc aagagcgacg
     1861 acgcgttctg gcgagacggg cggtacggcg aagcgtgggc gggacgccgg ttgtcgctca
     1921 ccgaggccag cgagaccttc gggctaccct gccggcacct gtacgacctg ccggaagcgc
     1981 tgcgcgcact cggccccgcc cgggtcctgc gtggagtcga cccggacgtc gacgcgctcg
     2041 tccccccgca acgtgacagc gcgggcgacc aggacctggc cgtgcacctg tcggagatgc
     2101 gcctggtcaa ggacgtctgg gagatcgagc agctgcagga cgccgtcgac tcgaccattc
     2161 tcgggttcga ggactgcgtc cgcgagtggg acgacgccgt gcggtacggc gaacgctgga
     2221 tcgagggaac gttctggcga cgcgcccgcg ccaacggcaa cgacgtgggt tacgagtgca
     2281 tctccgccgc cggcccgcat gccacgacgc tgcactggat cgacaacgac gggcctgtca
     2341 cgcccggaca cctggtcctg ctcgacatgg gcgtcgagaa ccgctcgttg tacaccgccg
     2401 acgtgacgcg gaccctcccg gtcgacggca cgttcaccgc cgaccagcgt cacctctacg
     2461 agctcgtgct cgcagcgcag gaggcgggca tggcagcagt gcgcccgggc gcggcgtaca
     2521 acgagttcca cctggcggcg atgggcgtgc tggcccgtgg cctttcggac atgggtatcc
     2581 tcccggtctc gccggaggag gcgttggaga gcgacagcgg cctgtaccgg cggtggacgc
     2641 tgcacgggac cggccacatg ctcggcctcg acgttcacga ctgcgcggct gcccggcgtg
     2701 agaactacaa cgacggcaag ctcgagaccg ggatggtgct cactgtcgag ccgggcctgt
     2761 acttccagag cagcgacctg ctggtgccgg agtcgctgcg cggcatcggc atccggatcg
     2821 aggacgacgt ggtggtgacc gacgacggct gccggaacct gtcgggtgcg ctcccccgca
     2881 cggccaccga cgtcgagtcg tggatggcag ggacggtgcg tcagaggtag aggccggtct
     2941 ggccgtcctc gaggcgctcg gaggcgacgg catggatgtc gcgttctctc agcaggatga
     3001 agtcctcgcc tctgacctcg acctccgcct tgtcctcggg gtcgaagagg acccggtcgt
     3061 cgacctggac agtccggaca ttggctccga cgcccaccac ccgggcccag gcgagtcgtc
     3121 ggcccatcac cgcggtggcg gggatcacga tgcccgccga ggagcgtcgc tcaccggcct
     3181 cgtggtccag cgagacgagc acgcggtcgt gcagcatgcg gatcggtagc ttgtccgccc
     3241 ccgggatgtt cgtgcgcgcc atgaagcccc tgatcctgtg aaccggcgtt cagtggacga
     3301 tcttgcggat gacgacgagc gccacgacga caccgatggc gatgccggcg accttcagca
     3361 cattgtccgt gcggaccgag ccgtcagggt tgacgaacga cgccttcacg ctccggaact
     3421 ggcgttcggc gatcgtcttg ggctgcaccc g
//
LOCUS       DATBFZ010000438         9437 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_3583634, whole genome shotgun sequence.
ACCESSION   DATBFZ010000438 DATBFZ010000000
VERSION     DATBFZ010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 9437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9437
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3583634"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="VH419_13325"
     CDS             complement(<1..422)
                     /locus_tag="VH419_13325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044645.1"
                     /translation="MLGLLGPIVATLWIAVRDNGPVGVVSDPLRDDLVTNAIGKTLWL
                     AVLVTAVTWLCGLLFTLGVALAPRWLGGLLFAILFITFWISLLVRTYGWVLVLQPNGA
                     LDDVGGWLSITDNGFGLFQTMPGLVPPMVHVMLPYMVLP"
     gene            complement(521..1642)
                     /locus_tag="VH419_13330"
     CDS             complement(521..1642)
                     /locus_tag="VH419_13330"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY7044646.1"
                     /translation="MAHVTDRRSFLKYALAGAGLIAAPNVLAACGGASTTTSSNGPTS
                     GAPEGIDAKWPDITSKQVVLAGFGGETYDVRRSLIFNSYTKLSGAEVVEAPWDYGKFL
                     NMVAAKNPEWDMIDFDGYSVVGLVEAGTPPAKLASWVRRCDLVDAAYQDYCAGSYAYS
                     VVLGWSSDLDTAPTNWADFFDTSKFPGKRAFPKSIYAGTVELALLADGVAPDQLYPLD
                     FDRGFAKLDTIKKDLLFYDSYAQGQQYMAQQSVSMIATANSRMIQDKAKGVGDFTYEQ
                     AILYPWGGFPITQNAPDMDAANALVDYMSDSSIQAAVARELYLGPIVSAAFDELTDDE
                     LALQPNSDENRAKAAVVDTKAAAQADAEYVKRFFSWVGQ"
     gene            1887..2804
                     /locus_tag="VH419_13335"
     CDS             1887..2804
                     /locus_tag="VH419_13335"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEY7044647.1"
                     /translation="MALDEVDLALLEGLRTDGRASYETLGAKAGVSRTAARARVHRLI
                     EDGVVNIEAIVHPAVEGIHTFAHVSIATDGGSAPEVAAKLAELDKAPLVSLVAGRWSM
                     IAELRTRDLGEMDVALAAVRDVPGVVSIDTTLYVDIVKDSHLPLGGTHSFEAFQLDDV
                     DHRLLDLLRADPRMPYADLADAVQLSSGATRARVLRMLRDSVVAVTGMINATTMGFTQ
                     MCGFQVHLADNGRSATDAVAALPQVDFLARTLGRSDLIGTVIATSRQDVHRTLDTIRT
                     FPGVRTVEAWYHLDLIKEWYEPRTSAAIA"
     gene            complement(2825..3790)
                     /locus_tag="VH419_13340"
     CDS             complement(2825..3790)
                     /locus_tag="VH419_13340"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-terminal binding protein"
                     /protein_id="HEY7044648.1"
                     /translation="MKAAEEVSGSPAVLYLDMTGLDVEPGQALLTAAGIEVMAAADDP
                     GPEAMDRVVALLTGYGEVTEDLLERLPALRLIATHSVGVDMVDIAAARARGVTVANVP
                     ASAVDEVAVHALALALALIRRIPAFDRDVRAGRWDRHTSPLPRVPGDMTCGVVGLGRI
                     GRRFAEVAIPLFGRVVGFDPGVPTHAWPETVESLEWAELLSVSDCLSLHLPLTSETAN
                     MLDENALQRLPPGAVVINVSRGALVDEKALVAALESGHVSGVGLDVRSAEPVPAGDAL
                     SNRADVVSTPHVAYLSPGSVRRYAEVPARNVIAMLEGGRPLHPVD"
     gene            3923..5524
                     /locus_tag="VH419_13345"
     CDS             3923..5524
                     /locus_tag="VH419_13345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044649.1"
                     /translation="MTVEALAWDWLREHDGDISAWHQHIWQLAEPAWREYESCRWYVE
                     TLRAHGFEVEEGSGGMPTAFHASWSQGDGPRLLTYAEYDAVPGNCQAATTRREPRPGL
                     SPFAPGHTDPHSALGISTLAGVLAAQHAMRERGIAGSLVYTGEPAEKMQGSKVVHGLR
                     GYYDGLDAILSFHPFYMLPLANTVRWDTHCGAYYSRIYSFVCDEPETWGAVYPGSPSP
                     ASHSAARAPGANSALATMYTLTRTTQDAMLPSVGGWSLTDAILTAGQATADNLPASLA
                     QLQYSWRCPDLAAAEAVLGVLDANADAAALATHCRVEKRWVARNRPGIPNHTLARVTW
                     EVMAEVGAPDLGPEAMQVARQIHSELGVETTEDPFLPETRDVLAPWKAEEQLRRLLPA
                     DQRNWTSDDYVEMTWYAPTVRFYMARPTLAPQPAVGAYPAWVMNALGGIPETIDPMIR
                     SASRVVAGTLLRLMDAPDVLRQAHEEHGRRRREWGDLSPLLPPDFAAPIDFRWPEYVE
                     TVRGREWWIPDSAEPWSQTRAKEHT"
     gene            5521..7158
                     /locus_tag="VH419_13350"
     CDS             5521..7158
                     /locus_tag="VH419_13350"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEY7044650.1"
                     /translation="MSSDPVMFTGGPIRLMDGAADSEPPEALLLEAGVVRAVGRAGDV
                     RATARSTPQEVDLDGRTLFPGFVDAHAHTLLHGCGVDWVDLSDAKTIDDVVERLRRRA
                     EERPGGSVHGYGYDQSKLAERRHPSARDLDEVTQERQVWIQHASGHGYVVNSPALDAA
                     GITAATSTPVGGRIDRDADGRPTGVVFDAACDLLTGPDGVKVSNHGPNFHLPMTDDEV
                     ARLFDLGQQSFLAAGITTICDAQVTELEMRAYLLARDDDRLRIRAHLLALSSRLDQLR
                     SLGLVSTLGDERLQLHGVKFYADGSVIARTAYLGGHSCCGKPEPTGYLYHDPAELTEL
                     ITTAHDLGLRTATHAQGDIPIGIVLDAVEQARDRDRRPGLVHRIEHCGFPTPEQIARM
                     SGLGVVPVPQPMQVHLYGDSLIEEYGDWGGSFYPYGSFEKHGLPVVVSSDAPVTMPAP
                     LQAAWAAVTRTTASGAVAGGVEQGASRTAAIEGTTVTPARLLGRSDVGTLGVGSRADL
                     VLLDSDPIDGSIDSLRMAQVTETWVGGDVAWSQARAS"
     gene            7155..8783
                     /locus_tag="VH419_13355"
     CDS             7155..8783
                     /locus_tag="VH419_13355"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEY7044651.1"
                     /translation="MTAGGDASAAGAPTTAAAATTGAAVVQALTANGVELVFGIPGTH
                     NLEIYRALPGSGIRHVTPRHEQGGGYAADAYARVSGRPGVVVTTTGPGLTNASTALAT
                     SYADSIPVLLISPGVPRGMERQDIGWLHELRDQHAHLAAIVDSHRVDSAAEAVAVVNN
                     AFANWRVARPRPVHVEIPLDVLDGEAQPADGTAVVPAATSPPSPSDDAVAAAADALAA
                     ASSVVIVAGGGARTATDELRAVAERLDAPVITTVNGKGVLPETHRLSLGASIRLPVAQ
                     QIISGSGALLVVGTVLGDDELWGQVVQHDGAVVRIDIDADQLHKNLPSTHPLHGAAGG
                     VLRRLEEALPAKPASSLGEQAGELRARCDEAALETGRPFAPWHAALREVLPPETVISG
                     DSAQVSYFGTAHQWPAQRPGQFVYPAGYATLGYGIPGGIGASLGAPGTPVVVVAGDGG
                     TMFTIQEFATAADLHLPLPVIVMNNGGFQEIREGMESRGMAPLAVDVRSPDFPLLGEA
                     LGGAGARAATPTELAAEVSKALSRDVPTLIDVPL"
     gene            8933..>9437
                     /locus_tag="VH419_13360"
     CDS             8933..>9437
                     /locus_tag="VH419_13360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HEY7044652.1"
                     /translation="MGTQLIHAAVLHGIGQTPRYEAFPSPVAGDEEEVVSVTAAALKP
                     SDRLMAAGVHYAPSRFPHVVGLDGVGRLPDGTRVAFFIPQAPYCGMAEQTLVRRGAWL
                     TIRDGVDDVTAAAVTNPGMAAWKTVEWEGELTADQTVLVLGATGTSGRIATQLAKRVG
                     ARVIAAGR"
BASE COUNT         1541 a         3245 c         3219 g         1432 t
ORIGIN      
        1 ggcagcacca tgtagggcag catcacgtgc accatgggcg gcacgaggcc gggcatggtc
       61 tggaagagcc cgaacccgtt gtccgtgatc gaaagccagc cgcccacgtc gtccagcgca
      121 ccgttgggct gcagcacgag cacccaaccg taggtccgga ccagcaacga gatccagaac
      181 gtgatgaaga ggatggcgaa cagcagccca ccaagccacc gaggcgccag tgccacgccc
      241 aacgtgaaca gcaacccgca gagccaggtc acggccgtga ccaggaccgc gagccaaagg
      301 gtcttgccga tcgcgttcgt cacgaggtcg tcgcgcagcg ggtcggagac gacgccgacc
      361 ggtccgttgt ctctgaccgc gatccacagc gtggcgacga tgggacccaa cagaccgagc
      421 accagcaaca ccgccggcgg aaggaggagc catcctcccc cggtgctgga gcgacgaacc
      481 cgaccaggcg ttctcccggt cggcgccagc gccgtctggg tcactgaccg acccagctga
      541 agaagcgttt cacgtactcg gcgtccgcct gggcggctgc cttggtgtcg acgacggcag
      601 ccttggcgcg gttctcgtcc gaattgggct ggagggcgag ctcgtcgtca gtcagctcgt
      661 cgaaggcggc ggagacgatc gggccgaggt agagctcccg ggcgacagcc gcctggatcg
      721 acgagtcgga catgtagtcg accagcgcgt tcgccgcgtc catgtccgga gcgttctggg
      781 tgatcgggaa tccgccccac gggtagagga tcgcctgctc gtaggtgaag tcacccacgc
      841 ccttggcctt gtcctggatc atccggctgt tcgcggtcgc gatcatcgag acgctctgct
      901 gcgccatgta ctgctgaccc tgcgcatagc tgtcgtagaa gagcaggtcc ttcttgatcg
      961 tgtcgagctt ggcgaagccc cgatcgaagt cgagggggta gagctggtcc ggagcgacac
     1021 cgtcggccag cagcgccagc tcgacggtgc ctgcgtagat cgacttgggg aatgcccgct
     1081 tgccaggaaa cttgctggtg tcgaagaagt ccgcccagtt ggtcggggcc gtgtccaggt
     1141 cgctcgacca gccgagcacc acgctgtagg cgtaagagcc ggcgcagtag tcctggtacg
     1201 ccgcatcgac caggtcacac cggcgtaccc agctggccaa cttggccggc ggtgtacccg
     1261 cctcgacgag gccgacgacc gagtagccgt cgaagtcgat catgtcccac tcggggttct
     1321 tggccgcgac catgttgagg aacttgccgt agtcccacgg cgcctcgacc acctctgcgc
     1381 cgctgagctt cgtgtaggag ttgaagatca aactgcgccg cacgtcatag gtctcaccgc
     1441 cgaagccggc aaggacaacc tgcttcgatg tgatgtcggg ccacttggcg tcgatgcctt
     1501 cgggcgcgcc gctggttggt ccgttgctcg acgtcgtcgt ggaggcgccg ccgcaggcgg
     1561 ccaacacgtt cggtgcggcg atcagaccgg cacctgccag cgcgtacttg aggaacgacc
     1621 tgcgatcggt gacgtgcgcc atcctgccct cctgggtcag ccgtcgaggc gtcgaagcct
     1681 cgtgtcgctg ctgcacgcta gcgcggtgca ggcagccctg taaatacctt ctcggtcgcg
     1741 aacttgcagt ttcagcgccg attcgtcact ggaacgggtc attctgcagt acagtgagcc
     1801 cggcgtcgcg gtccaaatcg ggccccggct ccccggccgg aacgggtgtc gtcccaccgg
     1861 atgctcggca cgaaaggcag agcgccatgg cactggacga ggtggacctc gcactgctcg
     1921 aggggctccg cacggacggc cgtgcgtcgt acgagacctt gggcgcgaag gccggggtgt
     1981 cacgaacggc ggcgcgagca cgggtgcacc gcctcatcga ggacggggtc gtcaacatcg
     2041 aggcgatcgt ccatccggcg gtggaaggca tccacacgtt cgcccacgtg tcgatcgcga
     2101 ccgacggcgg atcggcaccg gaggtggcgg ccaagttggc cgagctggac aaggctccac
     2161 tggtctccct tgttgcgggc cggtggtcga tgatcgcgga gctgcggacc cgagatctcg
     2221 gagagatgga cgttgccctc gcggcggtgc gggacgtacc cggcgtggtg tcgatcgaca
     2281 cgacgttgta cgtcgacatc gtgaaggact cgcatctgcc gctgggcggg acccactcgt
     2341 tcgaggcctt ccagctcgac gacgtcgacc atcgactgct cgacctgctg cgcgctgatc
     2401 cacgtatgcc gtacgccgac ctcgccgacg ccgtgcagct ctccagcggc gccactcggg
     2461 cacgggtcct gcggatgctg cgggactcgg tcgtcgcggt gaccggcatg atcaacgcga
     2521 cgacgatggg cttcacccag atgtgcggct tccaggtcca cctggccgac aacgggcgtt
     2581 ccgcgacgga tgccgttgca gcgctgcccc aagtcgactt cctggcgcga acactcggac
     2641 ggagcgacct gatcggcacg gtgatcgcca cgagtcggca agacgtccac cggacgctcg
     2701 acaccatccg cacgttcccc ggcgtccgaa ccgtcgaggc ctggtatcac ctggatctga
     2761 tcaaggagtg gtacgagccg cggacctccg ccgcaatcgc gtaggtcact cctggcaacc
     2821 aaagctagtc gaccggatgg agcggccggc cgccctccag catggcgatc acgttgcgcg
     2881 ccggcacctc ggcgtaacgg cggaccgagc ctggcgacag gtacgcaacg tgcggggtgc
     2941 tgaccacgtc ggctcggttg ctcagcgcgt cgcccgcggg caccggctcg gcagagcgca
     3001 cgtcgagtcc gacgccgctg acgtgtcccg actcgagcgc ggccaccaaa gccttctcgt
     3061 cgacgagcgc gccccgcgag acgttgatga cgacagctcc cggaggaagc cgctgcagcg
     3121 cgttctcgtc cagcatgttc gccgtctcac tggtcaacgg cagatgcagg ctcaggcagt
     3181 cactcacact gagcagctcg gcccactcca acgactcgac cgtctccggc caggcgtgtg
     3241 tcggcacacc ggggtcgaag ccgacgactc ggccgaacag cgggatcgcg acctcggcga
     3301 accgccgccc gatgcgaccg agcccgacaa ccccacaggt catgtcgccg ggcacgcgag
     3361 gcagcggact ggtgtggcgg tcccagcgcc cggcacgaac atcacgatcg aacgccggga
     3421 tgcgacggat gagcgcgagc gccagcgcca atgcgtgcac cgccacctcg tcgactgcac
     3481 tggccggcac gttggcaacg gtgacaccgc gggcgcgcgc agccgcgatg tcgaccatgt
     3541 cgacgccgac agagtgcgtg gcgatgagcc gcagggcggg cagccgttcg agcaggtctt
     3601 cggtcacctc gccgtagccg gtcaggagcg caaccacccg atccatcgct tcgggcccgg
     3661 ggtcgtctgc agcagccatg acctcgatgc ctgccgcggt cagcagtgcc tgtcccggtt
     3721 ccacatccag cccggtcatg tcgagataga ggacagccgg tgatccggag acctcctcgg
     3781 ctgctttcat atgtgccgtc cctccgtcgc agaactgcgt tttcggatgc ctgcgccttg
     3841 gttctcgaac gttaggtcta ccattgccac gctttcactg ctagcgtcac tggtcaacat
     3901 ctgactgctg gagggaccgc tggtgaccgt cgaggcgctc gcgtgggatt ggctgaggga
     3961 gcacgacggt gacatctccg cgtggcacca gcacatctgg cagctcgccg agccggcgtg
     4021 gcgcgagtac gagtcgtgcc gctggtacgt cgagacgctg cgcgcgcacg gcttcgaggt
     4081 cgaggagggc agcggcggta tgccgaccgc tttccacgcg tcgtggagcc agggcgacgg
     4141 accccgcctg ctgacctatg ccgagtacga cgcggtgccc ggcaactgtc aggcggccac
     4201 gaccaggagg gaaccacggc cgggcttgtc gccgttcgcc cccggacaca ccgatccgca
     4261 ttcggctctg ggcatctcca cgctcgcggg cgtcctcgcc gcccagcatg cgatgcgaga
     4321 gcggggcatc gcgggcagcc tcgtgtacac cggcgagccc gccgagaaga tgcaggggtc
     4381 caaggtcgtg cacgggttgc ggggttacta cgacggactc gacgcgatcc tcagcttcca
     4441 ccccttctac atgctgccgt tggccaacac ggtgcggtgg gacacccact gcggggccta
     4501 ctacagccgc atctacagct tcgtgtgcga cgagcccgag acctggggtg ccgtctatcc
     4561 gggcagcccc agtcccgctt cgcactccgc ggcccgcgct cctggcgcca actccgccct
     4621 ggcgacgatg tacacgctga cgcgcaccac gcaagacgcg atgctcccga gcgtcggcgg
     4681 atggtccctc accgacgcga tcctcactgc gggccaggcc acggccgaca acctgcctgc
     4741 ctcgcttgca cagctccagt actcgtggcg ctgtcctgac ctcgctgcgg cggaagcagt
     4801 cctcggcgta ctcgacgcga acgccgacgc agcggcgctg gcgacgcact gccgggtgga
     4861 gaagcgatgg gtggcgcgca accgtccggg gatcccgaac catacgctcg cacgtgtcac
     4921 ctgggaggtc atggcggagg tcggagctcc cgacctgggc cctgaggcga tgcaggtcgc
     4981 ccgacagatc cattccgaac tcggtgtgga gacgaccgaa gacccgttcc tgcccgagac
     5041 tcgcgacgta ctcgcaccat ggaaggcaga ggagcagctc cgacggctgc tgccagcaga
     5101 tcagcgcaac tggacctcgg acgactacgt ggagatgacg tggtacgccc caacggttcg
     5161 cttctacatg gcgcgcccga ccttggcgcc acagccggca gtgggcgcat acccggcgtg
     5221 ggtgatgaac gccctcggcg ggatcccgga aaccatcgac ccgatgatcc ggtcggcatc
     5281 gcgggtcgtt gccggcaccc tgttgcggct catggacgcg ccggacgtcc ttcgtcaggc
     5341 gcacgaggag cacggtcggc gacgacggga gtggggtgac ctctcaccgc tgctgccccc
     5401 agacttcgcg gccccgatcg acttccgttg gccggagtac gtcgagaccg tgcgtggccg
     5461 cgagtggtgg atcccggaca gcgccgaacc atggagccag acccgagcga aggagcacac
     5521 atgagttcgg acccggtgat gttcactggc gggcccatcc ggttgatgga cggtgcggcc
     5581 gacagtgagc cgcctgaggc cttgctcctc gaggcgggcg tcgtccgcgc cgtcggccgg
     5641 gccggcgacg tacgtgccac cgcgaggtcg acgccgcagg aggtggatct cgatgggcgc
     5701 accctgttcc ccggcttcgt cgacgcccac gcgcacaccc tcctgcacgg gtgcggcgtg
     5761 gactgggtgg acctcagcga cgccaagacg atcgacgacg tggtcgaacg gcttcgtcgg
     5821 cgagccgagg agcggcccgg cggatccgtc cacgggtacg ggtacgacca atcgaagctg
     5881 gccgagcgcc gacacccgtc ggcccgcgac ctcgacgagg tgacgcagga gcggcaggtc
     5941 tggatccagc acgccagcgg gcacgggtac gtcgtgaaca gtccggcgct ggacgcggcc
     6001 ggcatcaccg ccgccacgtc gacgcccgtt ggtgggcgga tcgaccgaga cgcagatggc
     6061 agaccgaccg gggtcgtgtt cgacgcagcc tgcgacctcc tgaccggccc ggacggcgtc
     6121 aaggtcagca accacggccc gaacttccac ctgccgatga ccgacgacga ggtggcgcga
     6181 ctcttcgacc tcggccagca gagcttcctc gccgccggga tcacgaccat ctgtgacgcg
     6241 caagtcaccg agctcgagat gcgggcgtac ctgctggcac gcgacgatga tcggctgcgc
     6301 atccgtgctc acctgttggc gttgtcgagc cggcttgacc agctccggtc actcggactg
     6361 gtgtcgacgc tcggcgatga gcggctccag ctccatggcg tgaagttcta cgcggacggt
     6421 tcggtgattg cccgaacggc gtacctcggc ggccactcgt gctgtgggaa gccggagccg
     6481 acgggctacc tctaccacga cccggccgag ctcacggagc tgatcacgac cgctcatgac
     6541 ctcggcctgc gaaccgcgac acacgctcag ggcgacatac cgatcggcat cgtgctggac
     6601 gccgtcgaac aggcccggga tcgtgaccgc cggccgggac tggtgcaccg catcgagcac
     6661 tgcggcttcc cgacacctga gcagatcgcc cgcatgtccg gtctcggcgt cgtgccggtg
     6721 ccacagccga tgcaggtgca cctgtacggc gactcgctca tcgaggagta cggcgactgg
     6781 ggcggctcgt tctaccccta cggatcgttc gagaagcacg gcctgccggt cgtcgtctcg
     6841 tcggacgcgc cggtcaccat gccggcgccg ctgcaggccg cgtgggctgc cgtcacccgg
     6901 acgacggcat caggagctgt cgccggcggg gtcgagcagg gcgcctcacg gaccgcggcg
     6961 atcgaaggca ccacagtcac accggcacgc ctgctcggtc gctccgacgt cggaacgctt
     7021 ggcgtcggca gtcgggccga cctggtcctc ctcgactccg atcccatcga cggctcgatc
     7081 gactccttgc ggatggcgca ggtcaccgag acgtgggtcg gcggcgacgt ggcctggagt
     7141 caggcgaggg cgtcgtgacc gccggcgggg acgcatccgc tgcgggagca ccgacgaccg
     7201 ccgccgctgc gaccacagga gcggccgtgg tgcaggcgct gaccgcgaac ggtgtcgagc
     7261 tcgtcttcgg catccctggg acccacaacc tggagatcta ccgggcgctg cccggtagcg
     7321 ggatccggca cgtgacgccg cgccacgagc agggcggcgg gtacgccgcc gacgcctacg
     7381 cgcgtgtgtc cgggcgacct ggtgtcgtcg tcaccacgac gggccccgga ctgacgaacg
     7441 ccagcacagc gttggcgacg tcatacgccg actccatccc ggtcctgctc atcagccccg
     7501 gagttccgcg cgggatggaa cggcaggaca tcggctggct tcacgagcta cgcgaccagc
     7561 acgcccacct cgctgccatc gtcgacagcc accgcgtgga ctcagccgcc gaggccgttg
     7621 ccgtcgtcaa caacgccttc gccaactggc gcgtcgctcg gcctcggccg gtgcatgtcg
     7681 agatcccgct ggacgtcctc gacggtgaag cgcagccggc cgacggaacc gctgtcgtcc
     7741 cggcggctac gtccccgcca agcccttccg acgacgcggt cgcagccgca gcggacgcct
     7801 tggccgccgc gtcatccgtg gtgatcgtcg ccggtggcgg tgcccggaca gcgacggacg
     7861 aactccgcgc tgtggccgaa cgcctcgacg caccggtcat caccaccgtc aacggcaagg
     7921 gagtgctgcc cgagacacat cggctctcct tgggagcgtc catccggctg ccggtcgccc
     7981 agcagatcat cagcggctcc ggcgccttgc tcgtcgtggg aacggtcttg ggagacgacg
     8041 agctctgggg ccaggtggtg cagcatgacg gagcggtcgt acggatcgac atcgacgccg
     8101 accagttgca caagaacctg ccctcgaccc atccgctgca cggtgcggcc ggcggcgtac
     8161 tccgacggtt ggaagaggca ctgcccgcca agcccgcgag cagcttgggt gagcaagccg
     8221 gcgagttgcg agcacgatgc gacgaggcgg cgctggagac cggccgaccg tttgctcctt
     8281 ggcatgccgc gctccgggag gtgctgccgc cggagacggt gatcagcggc gacagcgcac
     8341 aggtctccta cttcggcacg gcgcaccagt ggccggcgca gcgacccgga cagttcgtct
     8401 atccggcggg ctacgcaacg ctgggctacg gcatccctgg cgggatcggt gcctcactcg
     8461 gcgctcccgg cacgccggtg gtggtggtgg ccggtgacgg cggcacgatg ttcaccatcc
     8521 aggagtttgc aaccgccgcc gacctgcatc tgccactgcc cgtgatcgtc atgaacaacg
     8581 gcggcttcca ggagatccgc gaagggatgg agtcgcgagg catggcgccg ttggccgtcg
     8641 acgtacgcag cccggacttc ccgctcctgg gagaagcact tggtggggcc ggcgccagag
     8701 ccgcaacgcc gactgagctc gccgccgagg tcagcaaggc gttgtcgcgc gacgtgccga
     8761 cgttgatcga cgtaccgctg tagagcgtgg caaggacgcc tcgctgactc gccgcactgt
     8821 ctgagcgcgg cattctcagg cgaatccccg ctgtggctca gaccagtctc agcgtggcgg
     8881 atcagcgtgg gcggtgaccg ggtgttcgtc ccgctccccg caggaggcca tggtgggcac
     8941 gcaactgatc cacgccgcgg tgttgcacgg gatcggccag acgccgcgct acgaagcgtt
     9001 cccgtcaccg gtcgccggcg acgaggaaga ggtggtgtcg gtcactgcgg ccgctttgaa
     9061 gccgtccgac cggctgatgg ccgccggcgt ccactacgcg ccctcccggt tcccacacgt
     9121 ggtcggtctg gacggcgtgg gcaggctccc ggatgggacc agggtggcgt tcttcatccc
     9181 ccaggcgccg tactgcggga tggccgagca gactctcgta cgccgtggag cgtggctcac
     9241 gatccgcgac ggcgtcgacg acgtgacggc cgcggcggtc accaacccag gaatggccgc
     9301 ttggaagacc gtcgagtggg aaggcgagct gaccgccgac cagacggtcc tcgtcctcgg
     9361 cgccaccggg acgtccgggc gcatcgccac ccagctggcg aaacgagtgg gagcgcgggt
     9421 gatcgccgcc ggccgca
//
LOCUS       DATBFZ010000439         1914 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_4422093, whole genome shotgun sequence.
ACCESSION   DATBFZ010000439 DATBFZ010000000
VERSION     DATBFZ010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4422093"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1132
                     /locus_tag="VH419_13365"
     CDS             <1..1132
                     /locus_tag="VH419_13365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009077971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY7044653.1"
                     /translation="FHSFADSVAAAGQPSFMTDLAGGRQALNYAVRKPVGVVAVIVPW
                     NLPLLLLTWKVAPALACGNTVVVKPSEQTPSTATLLAEVLDDAGLPPGAYNVVHGFGP
                     GSAGQYLTEHPGIDAVTFTGSSATGAGIMRAVAARVRPVSFELGGKNAAVVFEDADLD
                     ATLDGLSRSVFANTGQVCLCTERVYVQRPIFDDVAAGLVERAKALRLGRPDDPATTTG
                     PLISAEHRDKVLSYYRLAEQDGAKTLVGGGVPSLESSLDGGSWIEPTLWTGLGNADRI
                     LREEIFGPCAALVPFDTEDEAVTLANDTDYGLASAVWTTNLSRGHRVAQRMRVGMSWV
                     NTWFLRDLRSPFGGVGLSGIGREGGEHSLDFYTELTNVCVQL"
     gene            1129..1908
                     /locus_tag="VH419_13370"
     CDS             1129..1908
                     /locus_tag="VH419_13370"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEY7044654.1"
                     /translation="MTEAIASAADRLLAAASSRQPCAPVRDLIGSTDVDAAYAVQQAL
                     TEARLAAGARITGRKIGLTSDAVRRQMSVFQPDFGVLFNDRGYASGETLPLSGLLQPR
                     VEAEIAFVLGRDLDLPHASVADVLRATEFVLAAIEVVDSRIAGWDITITDTVADNASS
                     GCYVLGTVARSLLGLDLARVGMVVEHDGSEVSAGSGASCMGSPVTAVAWLAREVARRG
                     MPLQEGEIVLSGALGPVVPVDKPGSFTARIDGFGEIEAVFA"
BASE COUNT          286 a          615 c          672 g          341 t
ORIGIN      
        1 cttccactcg ttcgccgatt cggtcgcggc cgccgggcag ccgtcgttca tgacggatct
       61 cgccggcggg cgacaggcgc tgaactacgc cgtgcgcaag ccggttggcg tcgtcgcggt
      121 gatcgtgccg tggaacctgc cgctgctgtt gctcacctgg aaggtggctc cggcactggc
      181 ttgcggcaac accgtcgtgg tcaagcccag cgagcagaca ccttcgaccg ccacgctgct
      241 cgccgaggtg ctcgacgatg ccgggctccc gcccggtgcg tacaacgtgg tccacggctt
      301 cggtcccggg tccgcgggcc agtacctgac cgagcacccc ggtatcgacg cggtgacctt
      361 caccggttcg tcggccacgg gtgccggcat catgcgtgcg gtcgcggcac gcgtccgtcc
      421 ggtgtcattc gagcttggtg gcaagaacgc ggcagtagtg ttcgaggacg ctgatctcga
      481 tgcgacgctc gacggactca gccgatcggt gttcgccaac accgggcagg tgtgcctgtg
      541 caccgaacgc gtatacgtcc aacgaccgat cttcgacgat gtcgccgccg gactggtgga
      601 gcgcgccaag gcgctgcggc tgggtcgccc cgatgatccc gcgacgacga ccggaccact
      661 catctccgcc gaacaccgtg acaaggtgct gagctattac cgacttgccg agcaagacgg
      721 tgccaagacg ctcgtcggcg gtggcgtccc ttcgctggag agcagcctcg atggtggctc
      781 gtggatcgaa ccgacgttgt ggacgggtct cggcaatgcc gaccgcatac tgcgcgagga
      841 gatcttcggc ccgtgtgcgg ccctggtccc gttcgacacc gaggacgagg cggtgacgct
      901 cgccaacgac accgattacg gtctcgcgtc ggcggtttgg acgacgaatc tgtcccgcgg
      961 gcaccgagtc gcgcagcgca tgcgggtggg catgtcgtgg gtgaacacct ggttcttgcg
     1021 ggacttgcgc tccccgttcg gtggggtggg gctgtcggga atcgggcgcg aaggcggcga
     1081 gcactcgctc gacttctaca ccgagctgac gaacgtgtgc gtgcagctgt gacggaggcg
     1141 atcgccagcg ccgcagaccg gctgctcgct gcggcctcat cacgtcagcc gtgtgcgccg
     1201 gtacgcgacc tgatcggcag cactgacgtc gacgcggcgt acgcggtcca gcaggccctg
     1261 accgaggccc gcctggccgc cggagcccgc atcacgggac gcaagatcgg cctgacctcg
     1321 gatgcagtgc ggcggcagat gtcggtgttc cagccggatt tcggcgtgct gttcaacgac
     1381 aggggttacg cctccggcga gacgttgccg ctctccggtc tgctacagcc acgcgtggag
     1441 gcagagatcg cattcgtgct gggacgggat ctcgacctgc cgcatgcttc ggtcgccgac
     1501 gtgctgcgcg cgacggaatt cgtgctcgct gcgatcgaag tcgtcgactc gcgcatcgcc
     1561 ggctgggaca tcacgatcac cgacacggtc gcagacaacg cgtcgagtgg ctgctatgtg
     1621 ctcggaaccg tggcgcgatc actgctcggg ctcgacctgg cgcgcgtcgg gatggtcgtc
     1681 gaacacgacg gtagtgaggt gtcggccggt tcgggtgcat cctgcatggg ctcgcctgtg
     1741 accgccgtgg cgtggctggc ccgcgaggtc gcgcgtcgcg gaatgccctt gcaagaaggc
     1801 gaaatcgtgc tgtcaggtgc tctcggtccg gttgtcccgg tggataagcc gggcagtttc
     1861 accgcgcgca ttgatggctt cggcgagata gaggcagtgt tcgcatgacc gtag
//
LOCUS       DATBFZ010000440         6729 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_4749589, whole genome shotgun sequence.
ACCESSION   DATBFZ010000440 DATBFZ010000000
VERSION     DATBFZ010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 6729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6729
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4749589"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            431..3778
                     /locus_tag="VH419_13375"
     CDS             431..3778
                     /locus_tag="VH419_13375"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY7044655.1"
                     /translation="MDLALLTDSDMRRRFGNSTYERGRQYADSGHVEEWKHVIDDDGD
                     LQARGQIAGSRNAPYTAYVAAGLDSEGLWFTSRCDCPMTSDCKHVVALLLTVRAELEG
                     GGRDPTAASDGRWERLLAPVLDELDDEASRAIGTPGLVPLGLQIDITQRHRSRSTYGR
                     WDVEGRSERRLGQGVLRIRPVKRGARDKWVKSGVSFQDLPGMGYDRSFDRSQVEALRE
                     VLALYRGGAAFGYYGGEAQLTLDAFGRSLWTGLARIERTGMPFVAGPGLDDVHVCHEP
                     VRLQLDVNAADGSGSHLRLGVEHEGTWYSGEALELIGQPGHGAALWTPGARADRWVVT
                     LVPLDRPVGPQLRRLVTAAEDLVVPRGALDDLVGDYLPRLQRHVTVVSSDGSVTVPEP
                     VAPRLAATVTWLAADEVRLAWTWRYRVGGSDRVYGLSETRGIRGVRRPELERDIVMGL
                     ALTDQQIFHLCDGHRLERGLCAERTFTHDRAVGFAEELLPTLEALAADRQIELDEVGG
                     RPDYRESSEAPVIRFAPRDPSSAKHQTRTDWLDLEVVISVDGQHLALARLLEALTLGH
                     ARVVMPNGVHIRLDRPEFTHLAELVQAARELHDQPREGMRVGSHDLGLWEELAELGIV
                     DAQAAQWAAAAQALRGLTTLPEVEPIGVKAELRHYQLEGFRWLAFLWESRLGGILADD
                     MGLGKTLQTLALVAYARDRGAGPFLVVAPTSVVSTWAHEAAQFCPGLVVRTVTQAATR
                     RGASISSLAEGADLLITSYTLFRLESDEYVDGSWGGLVLDEAQMVKNFRGKTHQSIRR
                     VEAPFRVALTGTPMENRLIELWALLSIVAPGLYSHPTRFKELVANPVEREHDAAALER
                     FRRRIRPFLLRRTKELVAADLPPKQEQVLDVALTPRHRRIYDTHLQRERQTVLGLVGD
                     FEKHRIAIFRSLTRLRQLSLDPALVDPRHDSVGSAKLDVLVDHLTEIMAEGHRALVFS
                     QFTSYLRRVRERFDREGIGSTYLDGRTRRRHEVIDAFKRGEGAVFLISLKAGGTGLTL
                     TEADYVFVLDPWWNPAVEAQAVDRAHRIGQRNPVMVYRLVSEDTIEEKVMELKARKAA
                     LFAKVVDGEGGAGTPISAADVRALFDD"
     gene            3920..4858
                     /gene="speB"
                     /locus_tag="VH419_13380"
     CDS             3920..4858
                     /gene="speB"
                     /locus_tag="VH419_13380"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009075607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HEY7044656.1"
                     /translation="MTEENVGPVDATVVPRYAGPPTFARLPRLDEVDRADVAILGVPF
                     DSGVSYRPGARFGPSHIRQSSRLLRPYNPAQDIHPFAVQQVADAGDVAANPFSIDEAI
                     QQIETGADATLEQAAKLLSLGGDHTIALPLLRVMHRRHGPVAVLHFDAHLDTWDTYFG
                     APYTHGTPFRRASEEGLIDHATSLHVGIRGPLYADTDLDESTRLGFATVNCTDYDRRG
                     LDGVISAMRDRLGNGPVYVSVDIDVIDPGMAPGTGTPEAGGLTSRELLTTLRGLAGLD
                     VVGADIVEVAPAYDHAEVTGIAAAHVGYELVSVMAG"
     gene            4883..6343
                     /locus_tag="VH419_13385"
     CDS             4883..6343
                     /locus_tag="VH419_13385"
                     /inference="COORDINATES: protein motif:HMM:NF014218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HEY7044657.1"
                     /translation="MSTQTESAPTRTSRLEVRSIDYVPLDERHGQLWHLGPLWFMSNA
                     QIATLVVGVLGVTTGGNLIWSLIAIVLGVLTGTFFMAAHSAQGPQLGLPQMIQSRPQF
                     GYVGAILVWAFAYIQYAGFNVFNTLLAGDGVHEAAVGPVKFWVIVATVLAVLAALVGY
                     DFIHRMEQSLTYAFLVLFGILTIVVFTLHYPDGSFDLGNFDKTPFLLQYSVVAGYQIS
                     WAIYVSDYSRYLPPDVTVRKTFWWTYGGSGLGAVWIMCVGSAIAAWAGASFDTIPSLK
                     AAGDEVFNGFGDVILVFSALGLISVTALNLYGGSLTLISASDSFKRIQPTLSVRVFTI
                     SLTAAISLVVALLSTESFLSNFEDFLLLVLYLFIPWTAVNLVDYFIVRKGHYAIAEIF
                     NPKGIYGRWGWNGIIAYVVGFAAMVPFFSTGIYSGPVADASGGADYSLFIGLPVSAIL
                     YYVLSRNIDVAAETEIAEREADELEREAHVHARPEA"
     gene            6446..>6729
                     /locus_tag="VH419_13390"
     CDS             6446..>6729
                     /locus_tag="VH419_13390"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEY7044658.1"
                     /translation="MDGLGDGWGMAEAVRRGEVTATQLVEDALARIAERDPEINAFTI
                     VLDDDARQAARAADELVRDFRAVPKVDADALPPLLGVPVSVKDHIWLADAP"
BASE COUNT         1047 a         2278 c         2329 g         1075 t
ORIGIN      
        1 cgcaaacctc cgatgggcac agggggtttg tcgtctgaac atcgcaaaca ctgggccggt
       61 gagacgcgca tgtccgaaac ccgccagcaa gacgccaggc aaggagggac cgtacgccga
      121 ccgaccagcc cagcaggcac gagcgtctcc cgctcgtctt tgccgactca cgtcagccga
      181 gaggacggta cgtcgggtga ccacgcgcac ccagcgcgac catctccgtc cgcagaaacc
      241 gaagactccc cagatctggc tggcggagcc ggcagtcgga taacggggat ggctcatcgg
      301 ttccgcagcg gcggcggtcc atggagaggc ggggcttcga aaggctcgag cgccgaagga
      361 ggctcggccg cgggcttcgc gacgtggcgt gccgccgctg tctgtccctc cgcataggct
      421 cggtggcgac atggatctcg ccctcctcac cgactccgac atgcgccgcc ggttcggcaa
      481 ctcgacgtac gagcgcggcc gccagtacgc cgacagcggc cacgtcgagg agtggaagca
      541 cgtcatcgac gacgacggcg accttcaggc acgcggccag atcgcgggat ccaggaacgc
      601 gccctacacg gcctatgtag cagcagggct ggacagcgag gggctgtggt tcaccagccg
      661 ctgcgactgc ccgatgacga gcgactgcaa gcatgtcgtc gcgttgctgc tgacggtgcg
      721 cgccgagctc gagggtggag gccgggaccc gaccgccgcg tcggacggcc gatgggagcg
      781 gctgctcgcg ccggtgctgg acgagctcga cgacgaggca agccgggcga tcggcactcc
      841 tggcctcgtt ccgctgggcc ttcagatcga catcacgcag cgtcacaggt ctcggtcgac
      901 gtacggccgg tgggacgtcg aagggagatc cgagcgacgt cttggccaag gtgtgctgcg
      961 gatccggccg gtaaagcgcg gtgcccgcga caagtgggtc aagtccggtg tgtccttcca
     1021 ggacctcccc ggcatgggat acgaccgctc cttcgaccgc agccaggtcg aggcgctgag
     1081 ggaggtcctc gcgctgtacc ggggtggggc agccttcggc tactacggcg gtgaggcgca
     1141 gctcacgctc gacgcgttcg gccggtcgtt gtggacaggg ctcgcgcgga tcgaacgcac
     1201 cggcatgccg ttcgtcgccg gcccgggtct cgacgacgtc cacgtgtgcc acgagccggt
     1261 ccggctgcag ctcgacgtca atgccgcaga cggttcggga tcccacctgc ggctcggcgt
     1321 cgaacacgaa ggaacgtggt actccggcga ggccctcgag ctcatcggtc aacccggtca
     1381 tggcgcggcc ctgtggacgc ccggcgcgcg tgctgaccgc tgggtggtca cgttggtgcc
     1441 gttggaccgt ccggtcggac cgcagctgcg gcggctggtc accgctgccg aggacctcgt
     1501 cgtaccacgc ggcgcgctgg acgacctggt cggcgactac ctgccccggc tgcagcgcca
     1561 cgtcacggtc gtgtcctccg acggttcggt cacggtgccc gagccggtgg ccccgcggct
     1621 cgccgcgacc gtcacctggc tggcggccga cgaggtgcgg ctggcctgga cctggcgcta
     1681 ccgggtcggt ggcagcgacc gcgtgtacgg cctttccgag acgcgaggta tccgcggcgt
     1741 acgccggccc gagctggagc gcgacatcgt gatgggtctc gcactgaccg accagcagat
     1801 cttccacctt tgcgacggac accggctgga gcgcgggttg tgcgcggaac gcacgttcac
     1861 gcacgaccgg gctgtcgggt tcgccgaaga gctgctgccc acgttggagg cgctggccgc
     1921 cgaccgacag atcgagctgg acgaggtcgg cggcagaccc gactaccgcg aatcgtccga
     1981 ggctcccgtc atccggttcg cgccccgcga cccgagcagc gccaagcacc agaccaggac
     2041 cgactggctc gacctcgagg tcgtgatcag cgtcgacgga cagcacctcg cgctggcacg
     2101 gttgctcgag gcgttgacgc tcggtcatgc acgggtggtg atgccgaacg gcgtccacat
     2161 ccggctcgac cgcccggagt tcacccatct cgcggagctc gtccaggcag cccgggagct
     2221 gcacgaccag ccgcgcgaag ggatgcgggt cggcagccat gacctcggtc tgtgggagga
     2281 gctggccgag ctcggcatcg tcgacgcgca ggcggcgcag tgggcggccg cggcgcaggc
     2341 gctgcgcggc ctgacgacgt tgccggaggt cgagccgatc ggcgtgaagg ccgaactgcg
     2401 gcactaccag ctcgaggggt tccgctggct ggcgttcctg tgggagtcgc ggctcggcgg
     2461 gatcctcgcc gacgacatgg gactcggcaa gacgctgcag acattggcgc tggtggcgta
     2521 cgcccgcgac cgcggcgccg gtccgttcct cgtcgtcgct cccaccagtg tcgtctcgac
     2581 ctgggcgcac gaggcggcgc agttctgtcc cggcctggtc gtccgtacgg tcacccaggc
     2641 ggctacccga cgtggtgcgt cgatctcgtc gctggccgag ggagcggacc tgctcatcac
     2701 gtcgtacacg ctgttccggc tcgagagcga cgagtacgtc gacggatcgt ggggtggcct
     2761 ggtgctcgac gaggcgcaga tggtgaagaa cttccgcggc aagacccacc agtcgatccg
     2821 ccgcgtcgag gcaccgttcc gcgtcgcctt gaccgggacg ccgatggaga accggctgat
     2881 tgagctgtgg gcgttgctgt cgatcgtggc gccagggttg tactcgcatc cgacccggtt
     2941 caaggagctg gtcgccaacc ctgtcgagcg cgagcacgac gccgcggcgc tggaacggtt
     3001 ccgccgccgc atccggccgt tcctcctgcg gcgtacgaag gagctggtgg cggccgacct
     3061 gccgccgaag caggagcagg tgctcgacgt ggcgctgact ccgcgccatc gacggatcta
     3121 cgacacgcac ctgcagcgcg aacggcagac cgtgctcggt ctggtcggcg acttcgagaa
     3181 gcaccggatc gcgatcttcc ggtcgctgac ccggctgcgg cagctgagcc tcgacccggc
     3241 gctggtcgac cctcgtcacg acagtgtcgg ttcggccaag ctcgacgtgc tcgtcgacca
     3301 cctcaccgag atcatggccg agggtcatcg ggcactggtg ttcagccagt tcacgtcgta
     3361 cctgcgccgc gtccgtgagc ggttcgaccg cgaaggcatc ggatccacct atctcgacgg
     3421 tcgcacccgc cgacggcacg aggtcatcga cgcgttcaag cgcggcgagg gtgcggtgtt
     3481 cctgatcagc ctcaaggccg gcgggaccgg tctgacgctg accgaggccg actacgtctt
     3541 cgtcctcgac ccgtggtgga accctgccgt cgaggcgcag gcggtcgaca gggcgcaccg
     3601 gatcggccag cgcaacccgg tcatggtcta ccgcctcgtc tccgaggaca ccatcgagga
     3661 gaaggtcatg gagctgaagg cgcgcaaggc cgcgctgttc gcaaaggtcg tcgacggcga
     3721 aggtggtgca ggtacgccga tcagcgccgc cgacgtacgc gccctcttcg acgactgacc
     3781 cccgccgagg tccccgcgct gatcgagctt gtcgggacca acggttcctt gtcgcgatcc
     3841 ttggttttcg acaggctcaa ccagcggatc gccgcgtctc gaggggctcg accgcccgag
     3901 agcggggaag atgaacggta tgacggaaga gaacgtggga ccggtcgatg cgacggtggt
     3961 gccgcggtac gccggtccac cgacattcgc gagactgccg cgcctcgacg aggtcgaccg
     4021 ggcggacgtg gccatcctcg gcgtgccgtt cgactccggt gtcagctacc ggccaggcgc
     4081 gcggttcgga ccgagccaca tccggcagtc ctcgcggttg ttgcggccgt acaacccggc
     4141 ccaggacatc cacccgttcg ccgtgcagca ggtggcggac gcgggcgacg tcgcagccaa
     4201 cccgttcagc atcgacgaag ccatccagca gatcgagacc ggcgccgacg cgacgctcga
     4261 gcaggcggca aagctgctgt cgctcggcgg cgaccacacg atcgcgctgc cgctgctgcg
     4321 cgtgatgcac cgccggcacg ggccagtcgc cgtcctgcac ttcgacgcgc acctcgacac
     4381 gtgggacacg tacttcggtg cgccctacac gcacggcacg ccgttcaggc gagccagtga
     4441 agagggcctg atcgaccatg cgacgtcgct gcacgtcggc atccgcggac cgctgtacgc
     4501 cgacaccgat ctggacgagt cgacccgcct cggcttcgcc accgtcaact gcaccgacta
     4561 cgaccggcgc ggcctggacg gcgtcatctc cgcgatgagg gatcggctcg gcaacgggcc
     4621 cgtctacgtc tcggtcgaca tcgacgtgat cgaccctggc atggcgcccg ggaccggcac
     4681 gcccgaggcc gggggcctga cgagccgcga gctgctgacg acgctgcgcg gcctggcggg
     4741 gctcgacgtc gtcggcgccg acatcgtcga ggtcgccccg gcgtacgacc acgccgaggt
     4801 gacggggatt gccgccgccc acgtggggta cgagctggtc agtgtcatgg cagggtagcc
     4861 gccgcatcga aaggacaccg ggatgagcac ccagaccgag tccgccccca cccgaacgag
     4921 ccggctcgag gtccggtcga tcgactacgt gccgctcgac gaacggcacg gccagctctg
     4981 gcacctcgga ccgttgtggt tcatgagcaa cgcccagatc gccacgctgg tggtcggcgt
     5041 gctgggggtc acgaccggcg gcaacctgat ctggtcgctg atcgcgatcg tgctcggcgt
     5101 actgaccggc acgttcttca tggcggcgca ctcggcgcag gggccgcagc tggggctgcc
     5161 gcagatgatt cagtcgcggc cgcagttcgg gtacgtcggc gcgatcctgg tgtgggcgtt
     5221 cgcgtacatc cagtacgccg ggttcaacgt cttcaacacg ctgctcgccg gagacggcgt
     5281 ccacgaagcg gcggtcgggc cggtgaagtt ctgggtcatc gtggcgaccg tcctcgcggt
     5341 ccttgcagcc cttgtcggct acgacttcat ccaccggatg gagcagagcc tgacctatgc
     5401 gttcctggtg ctgttcggca tcttgacgat cgtcgtcttc acgttgcact acccggacgg
     5461 ctccttcgac ctcgggaact tcgacaagac gccgttcctg ctgcagtact ccgttgtggc
     5521 cggctaccag atcagctggg cgatctacgt ctccgactac tcgcgctacc tcccacccga
     5581 tgtgaccgtt cgcaagacgt tctggtggac gtacggaggc tcaggtctcg gcgcggtctg
     5641 gatcatgtgc gtcggctcgg ccatcgcggc gtgggccgga gcgagcttcg acacgatccc
     5701 gtcgctcaag gcggctggtg acgaggtgtt caacgggttc ggcgacgtca tcctcgtctt
     5761 ctccgccttg ggcctcatct cggtgaccgc gctgaacctt tacggcggct cgctcacgct
     5821 gatcagtgcg tcggactcgt tcaagcgcat ccagccgacg ctctcggtgc gcgtcttcac
     5881 gatctcgttg actgctgcga tctcgctggt tgtggcgctg ctcagcacgg agtcgttcct
     5941 cagcaacttc gaggacttcc tgctgctcgt gctctatctc ttcatcccgt ggacggccgt
     6001 caacctggtc gactacttca tcgtccgcaa ggggcactac gcgattgccg agatcttcaa
     6061 ccccaagggc atctacggac gttggggatg gaacggcatc attgcgtacg tcgtggggtt
     6121 cgccgccatg gtgccgttct tcagcaccgg catctactcc ggcccggtcg ccgacgcgtc
     6181 gggcggtgcc gactactcac tgttcatcgg gctgccggtg tcggcgatcc tgtactacgt
     6241 cttgagccgc aacatcgacg tcgccgccga gaccgagatc gccgagcgcg aggccgacga
     6301 gctggagcgc gaggcgcacg tgcacgctcg tcccgaggcc tgacggcacc gaggtggtcg
     6361 aacggaccac aagccagcac tcaacgcacc acaacccagc actcaacgca ccggaaccca
     6421 gcactcaacg attagggtcg ggggggtgga tgggctgggc gacgggtggg ggatggcgga
     6481 ggccgtacgc cggggtgagg tcacggccac gcagctggtc gaggacgcgt tggcgcgcat
     6541 cgccgagcga gatccggaga tcaacgcgtt cacgatcgtg ctcgacgacg atgcgcgcca
     6601 ggcggctcgc gcggccgacg agctggtgag agacttccgc gcggtgccga aggtcgatgc
     6661 cgatgcgctg ccacccctgc tcggcgtacc ggtgtcggtg aaggaccaca tctggctcgc
     6721 cgacgcacc
//
LOCUS       DATBFZ010000441        18458 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_640072, whole genome shotgun sequence.
ACCESSION   DATBFZ010000441 DATBFZ010000000
VERSION     DATBFZ010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 18458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18458
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_640072"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(107..1264)
                     /locus_tag="VH419_13395"
     CDS             complement(107..1264)
                     /locus_tag="VH419_13395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008360194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-transfer protein"
                     /protein_id="HEY7044659.1"
                     /translation="MTTARIVGIGATEFSKHSGRSEIELSVDAIARALHDAGLEPHQV
                     QGLTTFTFDTSPESEISRNLGLGPLRFFAQSPFGGGGGCGAIMHAAMAIEAGVADVVA
                     CYRGLNERSGRRFGATARAVPARPAAQDILRGYYNQFGLITPADSFAMVARRYMHDTG
                     ATSEDFGRVAVVQRDYAVTNPAAWFYGRPLTLEQHQASRWIAEPLRLHDCCQESDGAV
                     AVVLASARVAATLEVTPVVVKAAAQSLPTGYEMMTDYNQPTLSALRETAELGEQLWSR
                     SGLGTDDVQLAILYDHFSPFVLLQLEALGFCGFGEAPELVACGETSPGGRLPVNTNGG
                     QLSEAYIHGMNGLAEAVRQLRGTAANQVSGVENVVVTSGPGLPTSGLILGL"
     gene            complement(1287..1793)
                     /locus_tag="VH419_13400"
     CDS             complement(1287..1793)
                     /locus_tag="VH419_13400"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HEY7044660.1"
                     /translation="MATDDAAPVRPRSGVNEDNEFWYEGIEQRELRIQSCADCGRLRH
                     PPCPMCAACHSLNRSWVVASGRGVIYSYVVHHHPPVVGLDTPYVVVLVELEEGIRVVG
                     NLLDGPHAGVAIGSPVELCFTADPRGGAVLPQWRLAGGAGEGAEPTSLVPPVHVADSD
                     SGSRLSAT"
     gene            complement(1799..3010)
                     /locus_tag="VH419_13405"
     CDS             complement(1799..3010)
                     /locus_tag="VH419_13405"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY7044661.1"
                     /translation="MTRLNRKSERRTMATELSEPLIDTDSHVIEPPDLWTSRVSAAWG
                     GQVPHVQFDEESGEDIWFIGDNAVGAAGHFAMVGWNDVWPSYPPRFDQVPTAAYDGAA
                     RLQLLDQAGIHAQLLYPNLIGGFLAHEFQKMEPRLALECIRAYNDFLTEFASPDPDRL
                     IALTALPFWDLDASVKEMERCRGLGHRGVLFAHTFEDVGLPNINDSHWHPVLDAAQSL
                     QQSINFHVGFAVYAKRSAKDLVSYSRDKRPVDIASGAALSFLSNARALTLVLLHGVCH
                     RYPDLKFVAVESGFGYVPYQLQALDWQWENNGLRAEFPERMPPSEYFARQVYCTLWFE
                     RPPLELLETWQDNVMWETDYPHPTAQWPTPTSRVAVDAHTAVEQSLGGLSDEARRKIV
                     STNAAALYRIG"
     gene            3043..4311
                     /locus_tag="VH419_13410"
     CDS             3043..4311
                     /locus_tag="VH419_13410"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY7044662.1"
                     /translation="MDGDEGQWRSGGSRPLRPYRICDLSGQLAGAGATKVLAAFGAQV
                     IRVEDPISSGRWDLFREMGPYADERRGVNLGGAFNNHNVEKLGVTINLRDRRGRELLG
                     RLIAASDVVTENFSAGVFARLGFSYEELRRLRPDIIYVSNSGFGHDGPYSGYKSWGPI
                     VQAVSGLTFEAGLPDREPAGWGYSLMDHVGASAGAMAILAALLHRERTGEGQAIDVAC
                     TEVAVQLLGPALMDVVANHRPRRRPGSPAGNRSEFRGMVPHGVFPVSGHDNWVAIACR
                     HDDDWLALRAVIGEAWALDPELETVAGRRAAEAEVDQRIAAWTGERTGASVTERLRAA
                     RVPVSIVARPEDRVEHDHSTRAAGLWTEVLHPEIGPVRVEGIPFHLSEDDWQISRPGP
                     CLGEHNDYVFGELLALSAESRGQLRDDGVI"
     gene            4313..5551
                     /locus_tag="VH419_13415"
     CDS             4313..5551
                     /locus_tag="VH419_13415"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY7044663.1"
                     /translation="MAPSALPFAGVRVVELASEATAFTGLLFAGQGAEVTVVEPLEGS
                     PTRSFGPFVEDEVGPDRSLWWRFYNRGKDGVRVDLDRPSGRAELVALLRDADVLLESE
                     STAHLAEVGLEWPDASSRFPRLIWVAMTPFGRQEPREDEPVTDLTMLARSGPIWSCGY
                     DDHDLPPVRGGEGHAAHIAGFQAVIGASIALLARLRSGRGQRVDVSLTATGNVTTEMS
                     TYEWLVRRATVQRQTGRHAQVVATQPTQVRARDGRYVNTGVPRATRTECVVLLHWMDE
                     LGVRDEFDDVGLLEIGAQGALPVEEPAIRTELIGAQRACISLIARHLDGQVFFVEAQE
                     RGLSAGVINAAEDVLTDPQFEARGMWVEVDEPSLGRSLNYPAAPLAFGGVRAVLGPAP
                     SFGGVSFGASEAAETATNPD"
     gene            5698..6981
                     /locus_tag="VH419_13420"
     CDS             5698..6981
                     /locus_tag="VH419_13420"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY7044664.1"
                     /translation="MARTEDDAALAVLDGLHIIDCDSHLTEPPDLWTSRVPDSAKDRV
                     PVQKTADGRTAWYLDGAPWASIGGNTIGTGLQKRLGTHMVQPFDDIDHSAWSVKERLA
                     LLDQIGVWAQILYPNGIGFSSNHVFAIEDETTRKLVLQTYNDFLVDTQLESNGRLFPQ
                     ALLPVWDIDFTVQEIARLVDKGITGFTMSDKPELIGLPELPEPYFDPMWDILNDTGTV
                     VNFHIGSGNRREETEAARASTSKAPGPLPPQRPGGPIPAAASPTWRSFGRQRGFAVLA
                     TQGYMSNVRIIVNLCMSNLFDRFDKLKIVSAESGIGWVPFVLEAMEFQLDEMVSEADE
                     VSYQQRRPTEYFRDHLYVMFWFEHSAPEKLIADIGVNNVLIETDIPHPTCLYPDPMAH
                     FARVLGHLPADMRRRVLQDNGSELYGIAVPAPAGT"
     gene            6994..7758
                     /locus_tag="VH419_13425"
     CDS             6994..7758
                     /locus_tag="VH419_13425"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HEY7044665.1"
                     /translation="MDEQRAGSPEPAPKISLRRTEKVSEVLAREIVRDLSDLPPATML
                     PSESAMLAKYGVGRASLREALRLLEVQGLIIMRPGPGGGPMVARADSAHYGRISSLYY
                     NLAGGTYQDIVDARLVIEPAIARMVAEQSDATQHEALRRYVDDRPQRDPVTSEARTVD
                     TAAEFHALLMGMCNNLVLQLLARSLQHVLLDRGRGVKPLLEWDRIEDDHATIARAIID
                     GDGDLTEELMRQHMAKWVASAKARHPGVLDEPVTWG"
     gene            7866..8780
                     /locus_tag="VH419_13430"
     CDS             7866..8780
                     /locus_tag="VH419_13430"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY7044666.1"
                     /translation="MGRLDGRTAIVTGAGRGIGASVARLLAAEGASVVVADLGVAIDG
                     SGRDPGPAEAVVNEIEAAGGVAVAAPVDVSSFEDAERLVSEAVDMFGGVDVLINAAGI
                     LRDRMIFNMSEEEWDTVIAVHLKGCFNTTRFVSAHWRQERKGDYRLINFTSIAGLNGR
                     PTGPNYAAAKMGIIGFTWSCANALGRYGATANCISPAASTRMADTIPPELLATYRAGP
                     GAASGDPKVNSPDNIAPAVAYLASKESGWLNGRIIGAQEYRISLWSNPEIHRQIVSDG
                     PWDLDDVFTMVPQAFQSTVEGVRRFDEA"
     gene            8782..9702
                     /locus_tag="VH419_13435"
     CDS             8782..9702
                     /locus_tag="VH419_13435"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEY7044667.1"
                     /translation="MAVSNDGRYLGVRSVIETPIMDDRKWAKIPDIRADGFLVDMEDS
                     VPPGLKEAARERVVEYVQRPEYFGGRLVVARVNHLSTPWGRDDIAALAEAGVTCVAYP
                     KLETPEDVVEVQELFRAGGADPDLFAPIESARAVLEVARIASIDKVVSVGIGVGDLSV
                     DMGVPLYGSDGQLNALFLGPKVQVALAGAAFGRLIGDMAFPADLRDLDEVRRQSEASR
                     HLGYNMGYTFYPPHVPVINDVFTPSKADADAADEVIGLYEAAVAAGDPAVTLPSGRTI
                     LVHDYRKALRMRAKLAAIRTREVDDPGART"
     gene            9809..11014
                     /locus_tag="VH419_13440"
     CDS             9809..11014
                     /locus_tag="VH419_13440"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY7044668.1"
                     /translation="MQSATPSPSNFDGLKVVEFAQIIAGPMAGTLLADLGAEVVHVEA
                     PGQGDPCRGMGPRKNDVHLWWKVSGRNKRSVTLDLRCAEGQAVARRLAGWADVVIANF
                     RVGTLERWGLTWDALHAVNPNLIMLQVSGHGANTTNRDDPGFGKVGEAMSGAVFVTGF
                     PDGPPVHTGFSHGDSVTALMGAYAISAALYRRNEPDFAGEWIDLALFETLFRLVEWQV
                     IAFDQVGMVPVRAGNQLSIAPGVVVNTYLTADNQWVTVTSATPRSVTNVAVLLGLPAD
                     EFLTSEQQFDRRRELDDGLREWVGARDIEQCLAEMKACDVVASRIYSVVDILEDQTYR
                     ERDDIVTVDDPDLGPVRMQAVIPKLLNRPGRVWRTGPALGEDNDLVYKKYLGVTEDEF
                     DALRANATI"
     gene            11020..11976
                     /locus_tag="VH419_13445"
     CDS             11020..11976
                     /locus_tag="VH419_13445"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY7044669.1"
                     /translation="MPTRSPSMYLATLDPELAAVLADRPLVETLSEETLAARRSVYPL
                     PLTEPLSERVSRIDHDVRPGGPVVRVHRPIELDGPLPCLYWMHPGGLVLGNRFQDDLR
                     FDVWCVRHRMVAVSVEYRLAPEAPYPAALDDCHDGLRWVLEQADELGVDPSRVGVGGA
                     SAGGGLAAALALLTRDRGTARPAFQLLIYPMLDDRLATRSSQADAPVWAPEENRFAWR
                     CYLGDRAGDDGVESYAAAARATDLSGLPPAYLLVGGLDGFLDETVEYATRLLHAGVAA
                     DLRVHAGLPHGFDGVAPRAAACRRAQRDLNDWLGRALHPEES"
     gene            11973..13667
                     /locus_tag="VH419_13450"
     CDS             11973..13667
                     /locus_tag="VH419_13450"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF012946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY7044670.1"
                     /translation="MNDTRPGQGEPDVVIVGAGFAGLYMLHRALRLGLSARVFEAAGD
                     VGGTWYFNRYPGARCDIPSLEYSYSFDEALQQEWRWSEKYSSQPQILEYLRHVCDRFG
                     LRPHIQFETRVTQATFDERVGRWRVQTDRGDDVRPQFLVMATGCLSTANIPDLHGLGS
                     FRGQVLHTGRWPQEPVDFSGQRVAVVGTGSSGVQVVPEIARAAAKLYVFQRTASYTLP
                     SSNGPLDPEEEASVKADYAAFRAANRRMPAATGARYLHAWHSISALEADPEERRRAFE
                     ETWTAGGFTFTHTYPDLVINRDANELAADFVRAKIRSIVHDPATAELLSPKLIIGCKR
                     MCIDSGYYETFNRPNVHLVDVGTQPITAATERGLLVGEAEYEVDSIVFATGFDALTGS
                     LLRVDIRGRAGHALADAWGGGPVTYLGLGVSGFPNLFLITGPGSPSALANVVVAAEQQ
                     IDWVGDCIAYMQAHGHRTIECSPDAQDAWVAHVGAVAAGSLRTDPSCNSWYLGSNIPG
                     KKRVFMLLIGFPPYVEKCDQVAADGYEGFMFERDGGDGYGKVGDETVASSSSAREG"
     gene            13669..14388
                     /locus_tag="VH419_13455"
     CDS             13669..14388
                     /locus_tag="VH419_13455"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009075199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEY7044671.1"
                     /translation="MSGRLAEKVCLITGGARGQGAAEAALFAAEGANVYVTDVLVDQG
                     EKTAADCGATFLRHDVTASADWDAVVERVVADFGRVDVLVNNAGIFKKVSLVDTTEDT
                     WGRFVAVNQTGPYLGMRAVAPHMIRQRSGSIVNVSSIAGLSGSFAFAYSTTKWAVRGM
                     TRSAAMELGPHGVRVNSVHPGIIDTAMIAEMNVETATQRVPLGRVAAPQEVAALVLWL
                     ASDESSYATGAEFVVDGGRTA"
     gene            14385..15587
                     /locus_tag="VH419_13460"
     CDS             14385..15587
                     /locus_tag="VH419_13460"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY7044672.1"
                     /translation="MTGPLEGIKVVEVGNAMAAPFCGMLLGDYGADVIKIERVGAGDD
                     SRGWPPYFYGRLSHYYAAANRNKRCVALDLKSAAGVDIAKRLIARADVLLENYRVGAL
                     ERAGLGYEAMADLNPRLIYCSVSGFGREGPRREERANDLMMQAFAGSMSVTGEYGGPP
                     VRMGTSVADIGAGLFATVGILSALESRHRSGRGQHVTTSLLQGQLAVLSYQLTSYFAT
                     GVPPIAYGAGAGLGVPYGAFPTADDPIIIAAFNDTMWRRLCAVLSHPEWADDPRFVDS
                     AHRVQHRDLLLGLVTEVLEAKPSAEWLAILQDAGVPCAPINRIDQVVVDPQVRSGGMI
                     AEMDVPDVGPVSFAAPPIGLSETPGEITLPPPRLGEHTRPILRDLGFDDAEVDRLAAD
                     GVVGLDPT"
     gene            complement(15626..16378)
                     /locus_tag="VH419_13465"
     CDS             complement(15626..16378)
                     /locus_tag="VH419_13465"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HEY7044673.1"
                     /translation="MSHIDREQGSIGTLGPRRTEKISEVLAREIVRDLRDLPPATMLP
                     SESVMLKKYGVGRASLREALRLLEVQGLVVIRPGPGGGPMVAQVDSTHYGRISTLYYH
                     MLGATYRDVVDARMVLEPVIARIVAERQDPEHIAKLNEWLDEVGSPGPHDYLGEASAF
                     HAMLVSMSGNPVLELTVHALQLIYAERAHGWVFPPEEHHHVEQAHRAIALAILKGNAS
                     RAEVLMRKHMLEYVENSRVNAPIGINETVDWH"
     gene            16626..17897
                     /locus_tag="VH419_13470"
     CDS             16626..17897
                     /locus_tag="VH419_13470"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEY7044674.1"
                     /translation="MSVDVGQESFDPLDAAVRRCPHPHYARMRAEAPVQYVDAVGMYF
                     VTRYDLVDQVLRDPHTFSSSRDKFAILRYRNPDDRAALAAVEAEGYPRVSTLIRVDPP
                     THTRYRRLVSKAFSPRAIAELEPVAREITTRLMDGWSDVEPVEFVSQFAVPLPLQVIA
                     RALNVPDDRLADFKRWSDNIVISTGANPSLEALLAAERGLNEFQHYFADALEQRRAHP
                     QDDLLTSLLNARIDDDDPDVTDKRPLDLPEMLNLIQQVLAGGNETTTKLLTEMMLLLA
                     EHPDVWSRMQREPSVIPAVVEESLRWVSVAQGMLRIATRTVELGGVEIPEGSTVVPMF
                     ASANRDEQVFDDPGRFEPDRGNLSEHVAFGKGPHYCLGASLTRLEVRVAMEELSRRVR
                     SFTLSDSNDFAYNPSFLLRGLVKLDLDLVMS"
     gene            complement(17919..>18458)
                     /locus_tag="VH419_13475"
     CDS             complement(17919..>18458)
                     /locus_tag="VH419_13475"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEY7044675.1"
                     /translation="SRPIVERTAAFNDGEVMNVIATVARHPKLLKRWTVFGNHILGGS
                     TLPARERELVILRTGFVASSPYEWVQHVKIARQAGCTDEEIERVMSGPDHPDWSDTDR
                     ALLEATDELMADRFISDQVWGALRSKWSEQQCMDLVFTVGQYCLVSMALNTFGVQIED
                     GVERFPSALFTDGPFSDVP"
BASE COUNT         2934 a         6324 c         6289 g         2911 t
ORIGIN      
        1 tcgcaccacg ctcgagatcg agtccacgtc gactcgacga cggtaggacg gaggccgtca
       61 cgagtccgtc accaccccca ggcgcgaggc cggtgccgag cgacgctcac aggccgagga
      121 tcagcccgct cgtcggcagg ccggggcccg acgtgacgac cacgttctcg accccgctga
      181 cctggttcgc cgcggtacca cgcagctggc ggaccgcttc ggcgaggccg ttcatgccgt
      241 ggatgtacgc ctcgctgagc tggccgccgt tggtgttcac ggggagccgg ccacccgggc
      301 tcgtctcgcc gcacgccacg agctcgggcg cctccccgaa gccgcagaag ccgagcgcct
      361 cgagttgcag gagcacgaaa ggtgagaagt ggtcgtagag gatggccagc tggacgtcgt
      421 ccgtgcccag ccccgagcga ctccagagct gctcgcccag ctcggccgtc tccctgagag
      481 cgctgagcgt cggctggttg tagtcggtca tcatctcgta gccggtcggg agcgattgcg
      541 cggcggcctt caccaccacg ggcgtcacct cgagcgtggc cgccacccgg gctgacgcga
      601 ggacgacggc caccgcgccg tccgattcct ggcagcagtc gtgaagccgg aggggctcgg
      661 cgatccacct cgacgcttgg tgttgctcga gcgtgagggg gcgtccgtag aaccaggctg
      721 ccgggttcgt cactgcgtag tcccgctgca ccacggccac ccggccgaag tcctcggagg
      781 tggcgccggt gtcgtgcatg taccgccgcg ccaccatggc gaacgaatca gccggcgtga
      841 tgaggccgaa ctggttgtag tagccgcgca ggatgtcctg ggccgccggc cgcgccggca
      901 cagcgcgcgc cgtggcgccg aagcggcgtc ccgagcgttc gttgagaccg cgatagcagg
      961 cgacgacatc ggcgacgccc gcctcgatcg ccatcgcggc gtgcatgatc gccccgcagc
     1021 ccccgccgcc gccgaacggc gattgggcga agaagcgcag cgggcccaga ccgagattgc
     1081 ggctgatctc gctctccggg ctggtgtcga acgtgaacgt cgtcagaccc tgtacttggt
     1141 gcggctcgag gccggcgtcg tggagcgccc gcgcgatcgc gtcgaccgag agctcgatct
     1201 ccgatcggcc cgagtgcttc gagaactcgg tcgcgccgat cccgacgatg cgcgccgtcg
     1261 tcacgggcgg tccctcacca acgggttcat gtcgcggaaa ggcgcgatcc gctgtccgaa
     1321 tccgcgacgt gaacgggtgg aacgagggac gtcggctccg cgccctcgcc agcaccgccg
     1381 gcgagccgcc actggggcag cacggcacca cccctgggat cggcggtgaa gcagagctcg
     1441 accgggctcc cgatggcgac gccggcgtgc ggtccatcga ggaggttccc gacgacacgg
     1501 atgccttcct ccagctccac gagcaccacg acgtacggcg tgtcgaggcc gacgaccgga
     1561 gggtggtggt ggacgacgta gctgtagatg acgccccgcc ccgacgcgac gacccacgag
     1621 cggttcagcg aatggcacgc ggcgcacatc ggacacggcg ggtgccgcag ccggccacag
     1681 tcggcacagg actggatgcg cagctcgcgc tgctcgatgc cctcgtacca gaactcgttg
     1741 tcctcgttga cgcccgaccg cggccggacc ggcgcggcgt cgtcggtcgc caaccgagtc
     1801 agccgattcg gtacagggcg gcggcgttcg tcgagacgat ctttcgcctc gcttcgtcgc
     1861 tcagcccgcc gaggctctgc tcgacggccg tgtgggcatc gaccgcgacg cgcgaggtcg
     1921 gcgtgggcca ctgcgcggtc ggatgcgggt agtcggtctc ccacatcacg ttgtcctgcc
     1981 acgtctcgag cagttcgagc ggcggccgct cgaaccacag cgtgcagtac acctgccggg
     2041 cgaagtactc gctgggcggc atgcgctcgg ggaactcggc ccgcagcccg ttgttctccc
     2101 actgccagtc gagcgcctgg agctggtacg gcacataacc gaagccgctc tcgacggcca
     2161 cgaacttcag gtccgggtag cggtggcaca caccgtggag aaggacgagc gtgagggcgc
     2221 gcgcattgct caagaagctc agcgcggcgc cgctggcgat gtcgacgggt cgcttgtcgc
     2281 ggctgtagct gaccaggtcc ttcgccgacc gcttggcgta gacggcaaag ccgacgtgga
     2341 agttgatcga ctgctggagt gactgcgcgg cgtcgagcac tggatgccag tggctgtcgt
     2401 tgatgttggg caggccgacg tcctcgaacg tgtgcgcgaa cagcacgccc cgatgaccca
     2461 ggccacgaca gcgctccatc tccttcaccg acgcgtcgag gtcccagaag gggagcgcgg
     2521 tgagcgcgat gagccgatca gggtcgggcg atgcgaactc ggtgaggaag tcgttgtagg
     2581 cccggatgca ctcgagggcc aggcggggtt ccatcttctg gaactcgtgc gcgaggaacc
     2641 cgccgatcag gttcgggtag agcagctggg cgtggatgcc ggcctggtcg agcagttgca
     2701 gacgcgccgc gccgtcgtag gccgccgtcg ggacctggtc gaaacggggc gggtagcttg
     2761 gccagacgtc gttccagccg accatggcaa agtggccggc cgcgccgacg gcgttgtcgc
     2821 cgatgaacca gatgtcctcg cccgattcct cgtcgaactg cacgtgcggg acctgaccgc
     2881 cccacgcggc ggaaacccgt gacgtccaaa ggtccggagg ctcgatcaca tggctgtcgg
     2941 tgtcgatgag cggttctgag agctctgtcg ccatcgtcct ccgctccgac ttcctgttga
     3001 ggcgcgtcac gccagccgag ccttactagt ataatgctta gcatggatgg ggacgagggt
     3061 caatggcggt caggcggctc acgacccctg cgtccctatc gcatctgcga cctctctggc
     3121 cagctcgctg gagcgggagc gaccaaggtg ctcgctgcct tcggcgcgca ggtcatccgc
     3181 gtcgaggacc cgatctcgtc cgggcgctgg gacctgttcc gcgagatggg gccgtacgcc
     3241 gacgagcgac gcggtgtcaa cctcggaggt gcgttcaaca accacaacgt cgagaagctc
     3301 ggcgtcacga tcaacctccg cgatcgccga ggccgggaac tgctcggccg cctgatcgcc
     3361 gcttccgacg tggtgacgga gaacttcagc gctggcgtct tcgctcgcct cggcttttcg
     3421 tacgaggaac ttcgccgact gcggcccgac atcatctacg tctccaactc cggcttcggc
     3481 cacgacggtc cctactccgg gtacaagagc tgggggccga tcgtgcaggc ggtcagcggc
     3541 ctgacgttcg aggccggcct gccggaccgc gaaccagccg ggtggggcta ctcgctgatg
     3601 gatcacgtcg gcgccagcgc cggtgcgatg gcgatcctcg ccgcgctctt gcaccgcgag
     3661 cgcacgggcg agggccaagc gatcgacgtc gcctgcaccg aggtggcggt ccagctgctc
     3721 ggaccagcac tcatggacgt cgtggccaac caccggccac ggcgtcgccc cggatcgcca
     3781 gccgggaacc ggagcgagtt ccgaggcatg gtgccccacg gcgtgttccc cgtatcaggc
     3841 cacgacaatt gggtggccat cgcctgccgc cacgatgacg actggctggc gctgcgcgcg
     3901 gtgatcggcg aggcctgggc gctcgatcct gagctggaga ccgtggccgg acgccgcgcc
     3961 gctgaagccg aggtcgacca acgcatcgcg gcgtggacgg gtgagcgaac tggagcgtcc
     4021 gtgaccgagc gcctacgcgc cgctcgcgtc ccggtgtcga tcgtcgctcg gccggaggac
     4081 cgcgtcgaac acgaccattc gacacgcgct gctggtctgt ggaccgaggt gctccatccc
     4141 gagatcggtc cggtccgggt cgaaggcatt ccgttccacc tgtccgagga cgactggcag
     4201 atcagcaggc ccggtccctg cctcggagag cacaacgact acgtgttcgg cgagctcctc
     4261 gctctgtcgg cggagtccag gggccaactc cgagacgacg gggtcatctg agatggcgcc
     4321 atcggcactc ccgttcgccg gcgtgcgggt cgtcgagctc gcatcggagg ccaccgcgtt
     4381 cacgggcctg ttgttcgcgg gccagggagc ggaggtgacc gtcgtcgagc cgttggaggg
     4441 gagcccgacg cggtcgttcg gaccgttcgt cgaggatgag gtcggccccg accgaagcct
     4501 ctggtggcgt ttctacaaca gagggaagga cggggtccgc gtcgatctcg accggccgag
     4561 cggccgcgct gagctggttg cgctgctgcg ggacgccgac gtcctgctcg agtcggagtc
     4621 gacggcccac ctcgccgagg tcgggttgga atggccggac gcgtcgtcac gctttccccg
     4681 cctgatctgg gtggcgatga cgccgttcgg ccggcaggag ccacgcgaag acgagcccgt
     4741 caccgacctg acgatgctgg cccgatcagg cccgatctgg agctgcggct acgacgacca
     4801 cgatctgccg ccggtgcgag gtggcgaagg gcatgccgcg cacatcgccg gcttccaggc
     4861 cgtcatcggg gcgagcatcg cgctcctcgc ccgattgcgg tccggtcgtg gccagcgagt
     4921 cgatgtcagt ctgaccgcga ccggcaacgt cacgaccgag atgtcgacct acgagtggct
     4981 ggtgcgccgg gcgaccgtgc agcgccagac cggccggcac gcccaagtcg tcgccaccca
     5041 accgacgcag gtgcgagcgc gcgacggacg atacgtgaac accggtgtcc ccagagcgac
     5101 tcggacggag tgcgtcgtcc tgctgcactg gatggacgag ctcggggtac gcgacgagtt
     5161 cgacgacgtc ggcctgctcg agatcggtgc gcaaggagcg ctgccggtcg aggaaccggc
     5221 catccgcacc gagctgatcg gtgcccaacg ggcgtgcatc agcctcatcg ctcgtcacct
     5281 cgacggccag gtgttcttcg tcgaggccca agagcgggga ttgtcggctg gcgtcatcaa
     5341 cgcggccgag gacgtgctca ccgatccgca gttcgaggcc cgcggcatgt gggtggaggt
     5401 cgacgagccg agtctcggtc ggtcgctcaa ctatccggcc gcgccccttg ccttcggtgg
     5461 tgttcgagcc gtgctcgggc cggcgccgag cttcggcggg gtctcgttcg gcgcgagcga
     5521 agcggccgag accgcgacca accccgacta gccgtactat tgctagagat tgggtgcgcc
     5581 cgtgaccggc ggcctcgcgg atttgacaac acctgctaat gctgcgcata gttagagttg
     5641 attgggccgc gaggcggcgc ggcgcctcat ttgatcagca ctcctgggag acaaggcatg
     5701 gcacggaccg aggacgatgc ggcgctcgct gtgctggacg gtctccacat catcgactgc
     5761 gactcgcatc tgaccgagcc tcccgatctg tggacgtcac gggttccgga ctcggcgaag
     5821 gatcgagtgc cggtgcagaa gacggccgat ggccgcaccg cgtggtacct cgacggcgct
     5881 ccttgggcga gcatcggcgg caacacgatc ggcaccggcc tccagaagcg tctcgggacc
     5941 cacatggtgc agcccttcga cgacatcgat cactcggcgt ggtcggtgaa ggaacgcttg
     6001 gcgctcctcg atcagatcgg ggtgtgggcg cagatcctgt acccgaacgg catcggcttc
     6061 tcgtccaatc acgtgttcgc gatcgaggac gagacgaccc gcaagctcgt gctccagacg
     6121 tacaacgact tcctggtcga cacgcagctc gagtccaacg gccgcctctt ccctcaggca
     6181 ctgctgccgg tgtgggacat cgacttcacg gtgcaggaga tcgcccgcct cgtcgacaaa
     6241 gggatcaccg gcttcacgat gtccgacaag cctgagctga tcggactgcc ggagcttcca
     6301 gagccctact tcgatccgat gtgggacatc ctcaacgaca ccggcacggt ggtgaacttc
     6361 cacatcggct cggggaaccg tcgggaagag accgaggcgg cacgagcgtc gacgtccaag
     6421 gcgcctggcc cgctgccccc acaacgaccg ggcgggccga tcccggcggc cgcttcaccg
     6481 acgtggcgca gcttcggtcg ccagcgcggc ttcgccgtgc tggccacgca gggctacatg
     6541 agcaacgtcc ggatcatcgt caatctgtgc atgagcaacc tcttcgaccg tttcgacaag
     6601 ctcaagatcg tctctgcgga gagcggcatc ggctgggtgc ccttcgtgct cgaggcgatg
     6661 gagttccagc tcgacgagat ggtgtccgaa gccgacgagg tcagctacca gcagcgccgt
     6721 ccgacggagt acttccgcga ccacctctac gtgatgttct ggttcgagca cagcgcaccc
     6781 gagaagctga tcgccgacat cggcgtgaac aacgtcctga tcgagacgga catcccgcat
     6841 ccgacttgtc tctatccaga cccgatggcg cacttcgcga gggtgctcgg tcatctcccg
     6901 gccgacatgc gccggcgcgt cctgcaggac aacggctccg agctctacgg gatcgcggtc
     6961 ccagcgcccg cgggcacctg agagcggcct ctcttggacg agcagcgcgc cggctcgccc
     7021 gagcccgccc cgaagatctc cctgcgccga actgagaagg tctcggaggt cctggcgcgg
     7081 gagatcgtcc gcgacctgag cgacctgccg ccggcgacga tgctgccgtc cgaatcggcg
     7141 atgctcgcca agtacggcgt cggacgagcg tctctgcgtg aagcgctccg actgctcgag
     7201 gtgcagggcc tcatcatcat gcggcccggt cccggcggcg gacccatggt ggcgcgagcc
     7261 gacagcgccc actacggacg gatctcgtcg ctctactaca acctcgccgg ggggacgtat
     7321 caggacatcg tcgacgcccg cctggtcatc gaaccggcca tcgcccgcat ggtggccgag
     7381 cagagcgatg ccacccaaca cgaagccttg cgcaggtacg tggacgaccg tccccagcgc
     7441 gacccggtca cgtccgaggc acgcacggtc gacacggccg ccgagttcca cgcgctgctg
     7501 atggggatgt gcaacaacct ggtgctccaa ctgctggccc gctcgctgca gcacgtgctg
     7561 ctcgaccgcg gccgaggcgt caaaccgttg ctcgagtggg accggatcga ggacgaccac
     7621 gcgacgatcg cgcgagccat catcgacggc gacggggacc tcacggagga gctcatgcgc
     7681 cagcacatgg cgaagtgggt cgcgtcggcc aaggcccggc accctggcgt gctcgacgag
     7741 cccgtgacct ggggctgacg cccgctcgcc gccggcgctg ctcggatgcg gcacgcttgc
     7801 agcgatcgcc gcctgttcct atcctggcga atcattgctc tagattcgga agacggaggt
     7861 gccgaatggg tcggctcgac gggaggaccg ccatcgtcac gggcgccggg cgcggcattg
     7921 gtgcctcggt ggccagactg ctcgccgcgg aaggtgcttc ggtggtcgtg gccgacctcg
     7981 gtgtcgcgat cgatggctcc ggccgtgatc cggggccggc agaggccgtg gtcaacgaga
     8041 tcgaagcggc cggtggcgtc gcggtggccg ctccggtcga cgtctcgagc ttcgaggacg
     8101 ccgagcgact cgtcagcgag gcggtggaca tgttcggcgg tgtcgacgta ctcatcaacg
     8161 ccgctgggat cctgcgagac cgcatgatct tcaacatgtc cgaggaggag tgggacacgg
     8221 tgatcgcagt gcacctcaag gggtgcttca acacgaccag gttcgtgtcc gcccactggc
     8281 gccaggagcg caagggcgat taccggctca tcaacttcac ctccatcgcc gggctgaacg
     8341 gccggccgac cggtcccaac tacgccgcgg cgaagatggg gatcatcggt ttcacctggt
     8401 cctgcgccaa cgccctcggc cgctacggcg ccacggccaa ctgcatctcc ccggccgcca
     8461 gcacgaggat ggccgacacc atcccgccgg agctcctggc gacctaccgc gccggtcctg
     8521 gtgcggcctc gggcgatccg aaggtcaact cgccggacaa catcgcaccc gcggtcgcct
     8581 atctggccag caaggagtca ggctggctca acggtcggat catcggcgcg caggagtacc
     8641 ggatcagcct ctggagcaac ccggagatcc accgccagat cgtctcggac ggcccctggg
     8701 acctcgacga cgtcttcacc atggtccccc aggcgttcca atcgaccgtc gagggcgtcc
     8761 gccgcttcga cgaggcgtga cgtggcggtc tcgaacgacg gccgatacct gggcgtgcgg
     8821 agtgtcatcg agacgccgat catggacgac cggaagtggg ccaagatccc cgacatccgc
     8881 gccgacggct tcctggtcga catggaggac agcgtgccgc ccggtctcaa ggaggcggcg
     8941 cgggagcggg tggtggagta cgtgcagcga cccgagtact tcggtggccg cctcgttgtg
     9001 gcccgcgtca accacctgtc gacgccctgg ggccgcgacg acatcgctgc cctggccgag
     9061 gccggcgtca cctgcgtcgc ctacccgaag ctcgagaccc ccgaggatgt cgtcgaggtc
     9121 caagagctct ttcgcgccgg cggggccgac cccgacctct tcgcgccgat cgagagcgcg
     9181 cgggccgtcc tcgaggtggc acggatcgct tcgatcgaca aggtggtgtc ggtcgggatc
     9241 ggcgtgggcg acctgagcgt cgacatgggg gtccccctgt acggctcgga cggtcagctg
     9301 aatgcgctgt tcctcggccc caaggtgcag gtcgctttgg ccggcgcggc cttcggccgg
     9361 ttgatcggcg acatggcctt ccccgctgac ctccgcgacc tcgacgaggt gcgacggcag
     9421 tcggaagctt cccgtcacct cgggtacaac atgggttaca ccttctatcc gccgcacgtg
     9481 ccggtcatca acgacgtctt caccccgtcc aaggctgacg ccgatgcggc cgacgaggtc
     9541 atcggcctct acgaagccgc ggttgccgcg ggcgatcctg ccgtcaccct cccatcgggc
     9601 cgcacgatcc tcgtgcacga ctaccggaag gcgctccgga tgcgtgcgaa gctcgctgcg
     9661 atcaggaccc gagaagtgga cgatcccggc gccaggacat aatctctgcc tataatacgt
     9721 cttgtctcgg cgacaagagt ggatgccgat cgcgcggtcg gccgggtcac cagcggaacg
     9781 ggtcctcgat cgacgagtgg aggtcaacat gcaatccgcc acgccgagtc cgtcgaactt
     9841 cgatggactg aaggtcgtcg agttcgcgca gatcatcgcc ggcccgatgg ccggcactct
     9901 cctggccgat ctcggcgccg aggtcgtcca cgtcgaggcg cccggacaag gtgacccctg
     9961 ccgaggcatg gggccgcgca agaacgacgt gcacctgtgg tggaaggtgt cgggtcgcaa
    10021 caagcgctcg gtgacgctcg acctccgttg cgccgaaggc caggccgtcg cccgacggct
    10081 ggccggctgg gccgatgtcg tgatcgccaa cttcagggtg ggcacgctgg aacggtgggg
    10141 cctcacgtgg gacgccctgc acgcagtgaa cccgaacctg atcatgctcc aggtgtccgg
    10201 ccacggggcg aacacgacga accgcgacga tcctgggttc ggcaaggtcg gcgaggcgat
    10261 gagcggtgcc gtgttcgtca ccggcttccc cgacgggccg ccggtgcaca ccggcttctc
    10321 gcacggtgac tccgtgaccg cgctcatggg cgcctacgcc atcagcgccg ccctctaccg
    10381 ccgcaacgag ccggacttcg caggggagtg gatcgacctc gcgctgttcg agacgctgtt
    10441 ccgcctcgtc gagtggcagg tgatcgcctt cgatcaggtc ggcatggtgc cggtgcgtgc
    10501 tggcaaccag ctctcgatcg ctccgggcgt cgtcgtcaac acctacctca ccgccgacaa
    10561 ccagtgggtc acggtgacct ccgcgacgcc gcgttcggtc acgaacgtcg cggtcctgct
    10621 cggcctgccc gccgatgagt tcctcacctc cgagcagcag ttcgaccggc gacgggagct
    10681 cgacgacggc ctgcgcgaat gggtgggagc gcgcgacatc gagcagtgcc tcgccgagat
    10741 gaaggcgtgc gacgtcgtcg cctcgaggat ctactcggtc gtcgacatcc tcgaggacca
    10801 gacgtaccgc gagcgcgacg acatcgtcac cgtcgacgac ccggaccttg gccctgtccg
    10861 catgcaggcg gtgataccga agctgctcaa ccgaccgggc cgcgtgtggc gcacgggacc
    10921 cgcgctcggt gaagacaacg acctggtgta caagaagtac ctcggcgtca ccgaagacga
    10981 gttcgacgcg ctacgggcca acgccaccat ctgagaacga tgccgacccg ctcgccctcc
    11041 atgtaccttg cgacgctcga cccggagctc gccgcggtcc tggctgaccg gccccttgtg
    11101 gagacgttgt cggaggagac actggccgcg cgccgctccg tctacccgct gcccctcacc
    11161 gagccgctgt ccgaacgggt gagccggatc gaccacgacg tgaggccggg aggaccggtc
    11221 gtccgggtgc atcggccgat cgagctcgac ggcccactgc cctgcctcta ctggatgcac
    11281 ccgggcggcc tcgtgctcgg caaccggttc caggacgatc tgcggttcga tgtgtggtgc
    11341 gtccggcacc ggatggtcgc ggtctcggtc gagtaccgcc tcgctcccga ggcaccgtat
    11401 ccggcggcgt tggacgactg ccacgacggc ctccgttggg tcctcgagca ggccgacgag
    11461 ctcggagtcg acccttcccg cgtcggcgtc ggcggagcga gtgcgggtgg gggtctcgcc
    11521 gccgcgctcg ccttgctcac ccgggaccgc ggcacggccc gtcccgcctt ccaattgctc
    11581 atctacccca tgctcgacga ccggctcgcc acccgatcga gccaggccga cgccccggtc
    11641 tgggcccccg aggagaaccg gttcgcctgg cgctgctatc tcggcgaccg cgccggggac
    11701 gacggtgtgg agagctacgc cgccgcggcc cgcgccaccg acctgtccgg actcccgccc
    11761 gcgtacctgc tcgtaggcgg cctcgacggg ttcctcgacg agacggtcga gtacgccacc
    11821 cgactgctgc acgccggtgt ggcggccgac ctgcgggtgc acgccggcct gccgcacggc
    11881 ttcgacggcg tcgctccgcg ggccgcagcg tgccgacggg cgcaacgcga cctgaacgac
    11941 tggctcgggc gcgccctcca tccggaggag tcgtgaacga cacgcgcccg gggcagggtg
    12001 agccggacgt cgtgatcgtc ggcgccggct tcgccggcct gtacatgctc catcgcgcac
    12061 tgcggctcgg cctgtcggcg cgggtgttcg aggcggccgg tgacgtcggt ggcacgtggt
    12121 acttcaaccg ctatcccggt gcccgctgcg acatcccgag cctcgagtac tcctactcgt
    12181 tcgacgaggc gctccagcag gagtggcgtt ggtcggagaa gtactcgtcc cagccccaga
    12241 tcctcgagta cctgcgccac gtgtgcgacc ggttcggcct ccgcccgcac atccagttcg
    12301 agacccgcgt gacgcaggcg acgttcgacg agcgcgtcgg gcgctggcga gtgcagacgg
    12361 atcgcggcga cgacgttcgg ccacagttcc tggtcatggc gaccggatgc ctctcgacgg
    12421 cgaacatccc cgatctgcat ggtctcggca gctttcgcgg ccaggtgttg cacacgggcc
    12481 gctggcccca ggagccggtc gacttcagcg gccagcgcgt cgccgtcgtc ggcacgggct
    12541 cctcgggcgt gcaggtggtc ccggagatcg cccgcgccgc ggcgaagctc tacgtcttcc
    12601 agcgcacggc gagctacacc ctcccctcga gcaacggtcc gctggaccca gaggaagaag
    12661 cgtcggtgaa ggcggactac gcggcgttcc gcgccgccaa ccggcgcatg ccggccgcca
    12721 cgggcgcccg ttacctccac gcgtggcact cgatctccgc gctggaggcc gaccccgaag
    12781 aacggcgccg ggcgttcgag gagacgtgga cggcgggcgg gttcaccttc acgcacacct
    12841 atcccgacct ggtcatcaac cgggacgcca acgagctggc cgccgacttc gtgcgagcaa
    12901 agatccgttc catcgtgcac gacccggcaa ccgctgagct gctgtcgccc aagctgatca
    12961 tcggttgcaa gcgcatgtgc atcgacagcg gctactacga gaccttcaac cgtccgaacg
    13021 tgcacctcgt cgacgtgggg acccaaccga tcacggccgc gaccgagcga gggctgctgg
    13081 tgggcgaggc cgaatacgag gtcgacagca tcgtcttcgc gaccgggttc gacgcgctga
    13141 ctggctcgct gctccgcgtc gacatccgcg ggcgcgccgg tcacgccctg gccgacgctt
    13201 ggggcggggg tccggtgacc tacctcggcc tcggcgtgag cgggttcccg aacctgttcc
    13261 tcatcaccgg accaggcagc ccctcggccc tggccaacgt cgtcgtggcg gccgagcagc
    13321 agatcgactg ggtcggcgac tgcatcgcct acatgcaggc acacggtcat cggacgatcg
    13381 agtgcagccc ggacgcacag gacgcatggg tggcgcacgt cggggcggtc gcggctgggt
    13441 cgctgcgcac ggacccgagc tgcaactcct ggtacctggg cagcaacatc ccaggcaaga
    13501 agcgggtgtt catgctcttg atcgggttcc cgccctacgt cgagaagtgt gatcaggtgg
    13561 cggcggacgg ctacgagggc ttcatgttcg agcgcgacgg tggcgatggg tacggcaagg
    13621 tgggcgacga gaccgtggca agcagcagct ccgcaaggga gggatgacat gagtggccgg
    13681 ctcgccgaga aggtgtgcct catcaccggt ggggcgcgcg gccagggtgc ggccgaggcg
    13741 gcgctgttcg cggccgaggg cgccaacgtc tacgtcaccg acgtgctggt ggaccaaggc
    13801 gagaagaccg cggccgactg cggcgcgacg ttcctccgcc acgacgtgac cgcgtccgcg
    13861 gattgggacg cggtcgtcga gcgcgtggtt gccgacttcg gccgtgtcga cgtgctcgtc
    13921 aacaacgccg gcatcttcaa gaaggtgtcg ctcgtcgaca ccactgagga cacgtggggt
    13981 cgcttcgtcg ccgtgaacca gaccgggccc tacctcggca tgcgcgccgt cgctccgcac
    14041 atgatccgcc agcgctcggg ttcgatcgtg aacgtctcgt cgatcgccgg tctgagcgga
    14101 tcgttcgcct ttgcctactc caccaccaag tgggcggtgc gaggcatgac gaggtcagcg
    14161 gcgatggagc tcggtccgca cggcgtgcgc gtcaactcgg tccatccggg catcatcgac
    14221 acggcgatga tcgccgagat gaacgtcgag acggcgacgc agcgggtgcc gctcggaagg
    14281 gtcgcggcac cgcaggaggt cgccgcgctc gtgctctggt tggcatcgga cgaaagcagc
    14341 tatgccaccg gcgcggagtt cgtcgtcgat ggaggacgga cggcgtgacc ggaccgctcg
    14401 agggcatcaa ggtggtggag gtcggcaacg ccatggccgc accgttctgc ggcatgctcc
    14461 tcggtgacta cggcgcggac gtcatcaaga tcgaacgggt cggcgccggc gacgacagcc
    14521 gcggttggcc gccgtacttc tacggccggc tgtcccacta ttacgcggca gccaaccgca
    14581 acaagagatg tgtcgccctc gacctgaagt cggcggccgg tgtcgatatc gccaagcggt
    14641 tgatcgcccg agccgatgtg ctgctcgaga actaccgcgt gggcgcactg gagcgtgccg
    14701 gcctcggcta cgaggccatg gccgacctca acccgaggct catctactgc tcggtcagcg
    14761 gcttcggccg cgaaggcccg agacgcgaag agcgggccaa cgacctcatg atgcaggcct
    14821 tcgccggcag catgagtgtc accggcgagt acggcggccc gccggtacgc atgggcacgt
    14881 cggtggccga catcggtgcc gggttgttcg ccaccgtcgg gattctcagc gccctcgagt
    14941 cccggcaccg cagcggccga ggccagcacg tgacgacgtc gttgctccag ggtcagctcg
    15001 ctgtcctctc gtaccagctg acgtcgtact tcgccaccgg tgtgccgccg atcgcctacg
    15061 gcgcgggcgc cgggctcggg gtcccgtatg gggccttccc gaccgccgac gacccgatca
    15121 tcatcgctgc cttcaacgac acgatgtggc gccgtctctg cgccgtgctg tcgcacccgg
    15181 agtgggccga cgatccgcgc ttcgtcgaca gcgcgcaccg cgtccaacat cgcgacctgc
    15241 tgctcggcct cgtgaccgag gtgctggagg ccaagccgtc agcggagtgg ctggccatcc
    15301 tccaggacgc cggcgtaccg tgcgcgccga tcaaccgcat cgaccaggtc gtggtcgacc
    15361 cgcaggtgcg ctcgggcggg atgatcgccg agatggacgt tccggacgtc ggcccggtca
    15421 gcttcgcggc cccgccgatc ggtctcagcg agacgccggg tgagatcaca ctgccgccgc
    15481 cgcggctcgg ggaacacacc cggccgatcc tgcgcgacct cggcttcgac gacgccgagg
    15541 tggatcgact cgcggccgac ggcgtggtgg gactcgatcc gacctgagct ccccgcgagc
    15601 ggccgccgag ccggccgccg tcgactcagt gccagtcgac ggtctcgttg atgccgatcg
    15661 gcgcgttgac gcgggagttc tcgacgtact ccagcatgtg cttccgcatc agcacctccg
    15721 cccgggatgc gttccctttg aggattgcga gggcgatggc ccgatgcgcc tgctcgacgt
    15781 gatggtgctc ctcaggcggg aacacccagc cgtgggcccg ctcggcgtag atcagctgca
    15841 gcgcgtgcac ggtgagctcc agcacggggt tgccggacat gctcacgagc atggcgtgga
    15901 aggcgctggc ctccccgagg tagtcgtgcg ggcccggcga gccgacctcg tcgagccact
    15961 cgttcagctt ggcgatgtgc tcaggatcct gccgctcggc gacgatccgg gcgatgactg
    16021 gctcgagcac catgcgggcg tcgaccacgt cgcggtacgt cgcgccgagc atgtggtagt
    16081 aaagcgtcga gatccgcccg tagtgggtgc tgtcgacctg agcgaccatc ggaccgcccc
    16141 cgggtccagg ccggatgacg acgagcccct ggacttcgag cagccgcaac gcctcgcgga
    16201 gcgacgcccg gccgactccg tacttcttca gcatcaccga ctctgacggc aacatcgtcg
    16261 ccggtggcag gtcgcgaagg tcgcgcacga tctcgcgcgc cagcacctcg gagatcttct
    16321 cggtacgtcg aggaccaagc gtgccgatcg agccttgctc cctgtcgatg tgcgacaccg
    16381 atcgcgctcc taaccgctcg cgcccaagcc gtgcgccccg gttgaacgag ggctattgaa
    16441 acgcaccaat acgatggtat cgagcatccg tcgacctctg cgggatggcg aacagcgctc
    16501 gagcccgatc agttgccgac tccgaagtac cggctgctgg ctatacgaat cattcgcatg
    16561 cattctgccg caggtgctac ccttcatgcg cggcaagggc gcgggcgtca tcgaaagggg
    16621 atcggatgtc ggtcgatgtc gggcaggagt cgttcgatcc gctcgatgcg gcagtccggc
    16681 gctgcccgca tccgcattac gcgcgcatgc gagccgaggc gccggtgcag tacgtcgacg
    16741 ccgtgggcat gtacttcgtg acccggtacg acctcgtgga ccaggtgctg cgcgaccccc
    16801 acaccttttc gtcgtcgcgt gacaagttcg ccatcctccg ctaccggaac cccgatgacc
    16861 gcgctgctct cgccgcggtc gaggcggagg gctacccgcg cgtgtcgacc ctcatacggg
    16921 tcgatcctcc gacccacacc cgttaccggc gactcgtctc gaaggcgttc agtccgcgcg
    16981 ccatcgccga gttggagccg gtggcccgcg agatcacgac gcgactcatg gacgggtgga
    17041 gcgacgtcga gccggtcgag ttcgtcagtc agttcgcggt tccactcccg ctgcaggtga
    17101 tcgcccgagc gttgaacgtc ccggacgacc ggctcgccga cttcaagcgg tggtccgaca
    17161 acatcgtcat ctccaccggc gccaacccgt cgttggaggc tctgctggcc gccgagcgcg
    17221 gcctcaacga gttccagcac tacttcgcgg atgcactcga acagcgccga gcacacccgc
    17281 aggacgacct gctcaccagc ctgctcaacg ctcgtatcga cgacgacgat ccagacgtca
    17341 cggacaagcg cccgctggac cttcccgaga tgctcaacct catccaacag gtgctggcgg
    17401 gcgggaacga gaccacgacg aagctgctca ccgagatgat gctcctcctc gccgagcacc
    17461 cggacgtgtg gtcacgcatg cagcgcgagc cgtcggtgat cccggctgtc gtcgaggagt
    17521 cgctccgctg ggtctccgtc gctcagggga tgttgcggat cgcgacgagg acagtggagc
    17581 tcggtggagt cgagatcccc gagggcagca cggtcgtgcc gatgttcgcg tctgcaaacc
    17641 gcgacgagca ggtcttcgac gaccctggcc gcttcgagcc cgaccgaggc aacctgagcg
    17701 agcacgtcgc cttcggcaag ggaccgcact actgcctcgg tgccagcctc acccggttgg
    17761 aggtccgcgt ggcgatggaa gagctgtcgc gacgggtgcg gtcgttcacg ctctccgact
    17821 ccaacgactt cgcctacaac ccgagcttcc tgctccgcgg gctggtcaag ctcgacctcg
    17881 acctcgtcat gagctgacca gcactggtcg tcgccggctc aggggacgtc ggagaacggc
    17941 ccgtcggtga acagcgcgct cgggaaacgc tcgacgccgt cttcgatctg cacgccgaag
    18001 gtgttcagag ccatcgagac gaggcagtac tgaccaacgg tgaagaccag atccatgcac
    18061 tgctgctcgg accacttcga ccgcaaggcg ccccagacct ggtccgagat gaaccggtcc
    18121 gccatcagct cgtcggtggc ctcgagcagg gcgcggtcgg tgtccgacca gtcggggtgg
    18181 tccggtccgg acatgacccg ctcgatctcc tcgtccgtgc agccggcctg gcgagcgatc
    18241 ttgacgtgct gaacccactc gtacggggag gaggcgacga agccggttcg gagaatgacg
    18301 agctcgcgct cgcgggccgg cagggtcgag ccacccagga tgtggttgcc gaagacggtc
    18361 caccgcttga ggagcttggg gtgccgagcc accgtagcga tcacgttcat gacctcaccg
    18421 tcgttgaagg ccgccgttcg ttcgacgatc gggcgact
//
LOCUS       DATBFZ010000442         5335 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_5406374, whole genome shotgun sequence.
ACCESSION   DATBFZ010000442 DATBFZ010000000
VERSION     DATBFZ010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 5335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5335
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5406374"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="VH419_13480"
     CDS             <1..471
                     /locus_tag="VH419_13480"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-guanine transglycosylase"
                     /protein_id="HEY7044676.1"
                     /translation="GIGGALEKASLGTITGWVSSELPEDKPRHMLGISEPDDFFACIE
                     NGADTFDCVNPSRVARNAAVYTRTGRFNVNTAASRRAYVPIDAECDCYTCAHYTRAYL
                     HHLFKAKEMLASTLCTIHNEWFVVSLVDRIRAAIDDGTYEAVKAETLGRYYARR"
     gene            complement(538..1659)
                     /locus_tag="VH419_13485"
     CDS             complement(538..1659)
                     /locus_tag="VH419_13485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HEY7044677.1"
                     /translation="MTSLQQTRSVPATTGPRYVRHPTGIYTVIVALFCGLLLISNIAA
                     TKLITVVDGLPVIQGIFTDGGAFLFPLTYIFGDVLAEVYGMRQARRAIWLGFFLAGLA
                     SLTFLVVGLAPPGPGYENQDAFVAVLGFVPRIVVASLAGYLAGQFLNAYVLVKIKSRT
                     DERKLWVRLVGSTVVGELADTALFCFIAFAGVIAGWGALISYTITGYLYKVMVEVIFL
                     PVTYAVIRAIKKREPDYAAARTEKQGVHQLRLVVRAEDYDDAVRFYRDTLGMPEEGSY
                     NTGDARVMILDAGRATLELSNPAQVDYIDTAEVGRMGVSPPWRVALEVDDAAATTRRL
                     EYAGAKVIAEPVETPWRSLNARLSAPAGVQLTIFTELDR"
     gene            1715..2113
                     /locus_tag="VH419_13490"
     CDS             1715..2113
                     /locus_tag="VH419_13490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5709 domain-containing protein"
                     /protein_id="HEY7044678.1"
                     /translation="MSEIYESVPEETEQLDQDQLQPDVNLNNRGVDDALDEGYSPPES
                     YSTLVRHGTHETLDERLAEEEPDVAYDDDEYDDYVDDGEVGGARAGRLVDPNEGIGPD
                     DEKEMIGEDVGIDGAAASAEEAAMHIVGEE"
     gene            2110..3090
                     /gene="pip"
                     /locus_tag="VH419_13495"
     CDS             2110..3090
                     /gene="pip"
                     /locus_tag="VH419_13495"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HEY7044679.1"
                     /translation="MTLPRQPAESGWPITEPYDRGLLDVGDGQQISWEEYGNPDGKPA
                     VFLHGGPGGGINPGQRRLFDPDRYRVVAFDQRGCGRSLPHASAAGADLSTNTTWHLVA
                     DLERLREARGIDRWLVCGGSWGSTLALAYAETHPERTSELVLRGIFTLRRSELDFYYN
                     GGAGQLFPDRYEAFLAPLGGRGFSGDAIAAYHELLFDPDPAVHGPAGVAWSSWEAATI
                     TLEPNEELIATFSEPSYALAFARIENHYFVHGGWFADNQLIANADRLTGIPGVIVQGR
                     YDVATPAATAWDLHRAWPEADFRIMPVAGHAYSEPQIFAELIAALDRFAA"
     gene            3355..3981
                     /locus_tag="VH419_13500"
                     /pseudo
     CDS             3355..3981
                     /locus_tag="VH419_13500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007078270.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
     gene            5059..5335
                     /locus_tag="VH419_13505"
                     /pseudo
     CDS             5059..5335
                     /locus_tag="VH419_13505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007589577.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
BASE COUNT          919 a         1838 c         1750 g          828 t
ORIGIN      
        1 gggatcggcg gcgctctcga gaaggccagc ctcggcacga tcaccggctg ggtcagctcg
       61 gaactgcccg aggacaagcc ccggcacatg ctcggcatct ccgagccgga cgacttcttc
      121 gcctgcatcg agaacggcgc cgacaccttc gactgcgtca atccctcccg ggtcgcccgc
      181 aacgcggccg tctacacccg aaccggccgg ttcaacgtca acaccgccgc gtcgcgccgg
      241 gcgtacgtcc ccatcgacgc cgagtgcgac tgctacacgt gcgcccacta cacccgggcg
      301 tacctgcacc atctgttcaa ggccaaggag atgctggcat cgactctctg caccatccac
      361 aacgagtggt tcgtggtctc cctcgtcgac cggattaggg cagccatcga cgacggcacc
      421 tacgaggcgg tcaaggcgga gaccctgggc cgctactacg cccgacgatg acgcggcttc
      481 cctcgacgtg tcagaactac aactggctac agcacgctga ggttctgaca agtcgggcta
      541 tcggtcgagc tcggtgaaga tcgtcagttg tacgccggcg ggggctgaga gccgggcgtt
      601 cagcgagcgc cagggcgtct cgaccggctc cgcgatcacc ttcgctccgg cgtactccag
      661 ccgacgagtt gtcgccgcgg cgtcgtcgac ctcgagcgcc acccgccagg gcgggctgac
      721 ccccatgcgt cccacttcgg cggtgtcgat gtaatccacc tgggccgggt tggacagctc
      781 cagggtcgca cggccggcgt ccaagatcat cacccgggcg tctccggtgt tatagctccc
      841 ctcctcgggc atgccgagcg tgtcgcggta gaaccggacc gcgtcgtcgt agtcttccgc
      901 ccggacgacc agccgaagct ggtgcacccc ttgcttctcg gtgcgtgcgg cggcgtagtc
      961 cggctcccgc ttcttgatcg ccctgatcac cgcgtaggtg accggcagga agatcacctc
     1021 gaccatcacc ttgtagagat agccggtgat cgtgtagctg atcaacgcgc cccacccggc
     1081 gatcaccccg gcgaacgcga taaagcagaa caaggcggtg tcggccagct caccgaccac
     1141 ggtcgagccg accagccgca cccacagctt gcgttcgtcg gtccggctct tgatcttgac
     1201 caggacgtac gcgttcagga actggccggc gaggtagccg gccaggctgg cgacgacgat
     1261 ccgcggcacg aagccgagca cggcgacgaa cgcgtcctgg ttctcatatc ccggacccgg
     1321 cggggcgagg ccgacgacga ggaacgtgag cgatgccagg ccggcgagga agaagcccag
     1381 ccagatcgcc cgccgggcct ggcgcatgcc gtacacctcc gccagcacgt cgccgaagat
     1441 gtaggtcagc gggaacaaga acgcgccgcc gtcggtgaag atcccctgga tcaccggcag
     1501 cccgtcgacg accgtgatca acttcgtggc ggcgatgttg gagatcagca gcagaccgca
     1561 gaacaaggcg acgatcacgg tatagatgcc ggtcggatgt cggacgtacc tcggaccggt
     1621 cgtcgcgggg acggaacggg tctgttgcag cgaggtcacg tccggcaagc ctacggttct
     1681 gggccgtcgg gagcacacat ggcaggctgg agccatgagc gagatctacg agagcgttcc
     1741 cgaggagacc gagcagctcg accaggacca gttgcagccc gacgtcaacc tgaacaaccg
     1801 gggtgtggac gacgcgctcg acgagggata ctcgccgccg gagagctact ccacgctcgt
     1861 gcggcacggc acccacgaga cgctcgacga gcggctggcc gaggaggagc ccgacgtcgc
     1921 ctacgacgac gacgagtacg acgactacgt cgacgacggc gaggtcggcg gtgcacgtgc
     1981 cggccggctg gtggacccga acgaaggcat cggccccgac gacgagaagg agatgatcgg
     2041 cgaggacgtc ggcatcgacg gcgccgcggc gagcgccgag gaagccgcca tgcacatcgt
     2101 cggtgaggag tgacgcttcc ccgacagccg gcggagagcg gttggccgat caccgagccg
     2161 tacgaccgag ggctgctcga cgtcggcgac ggccagcaga tctcctggga ggagtacggg
     2221 aatcccgacg gcaagccggc ggtcttcttg cacggcggac ctggcggcgg catcaacccc
     2281 ggccagcggc ggctcttcga ccccgaccgc tatcgggtgg ttgcgttcga ccagcgcgga
     2341 tgcgggcgga gcctgccgca cgccagcgcg gccggagccg acctcagcac caacacgacc
     2401 tggcatctgg tcgccgacct ggaacggctc cgggaggcgc gcgggatcga ccgctggctg
     2461 gtctgcggcg gctcgtgggg tagcaccctg gccctggcgt acgccgagac ccaccccgag
     2521 cgaacttccg agctcgtgct ccgcggcatc ttcaccttgc ggcgcagcga gctggacttc
     2581 tactacaacg gcggcgccgg ccagctcttc ccggaccgct acgaggcgtt cctggccccg
     2641 ctcggcggcc gcggcttctc cggtgacgcg atcgcggcct accacgagct gctcttcgat
     2701 cccgacccgg ccgtgcacgg gccggcgggc gtggcctgga gcagctggga ggcggcgacg
     2761 atcaccctgg agccgaacga ggagctgatc gcgaccttca gcgagccctc gtacgccctc
     2821 gccttcgccc ggatcgagaa ccactacttc gtccacggcg gctggttcgc cgacaaccag
     2881 ctgatcgcga acgcggaccg gctgaccggc atccccggtg tcatcgtcca gggacgctac
     2941 gacgtcgcaa ccccggccgc gaccgcatgg gatctgcatc gagcctggcc agaggccgat
     3001 ttccggatca tgccggtcgc cgggcacgcg tactccgagc cgcagatctt cgccgagctg
     3061 atcgccgccc tcgaccgatt cgccgcctga cgcccccttt gcgccgagtt gtcaggttct
     3121 cagcgagcca gaactcgctg agaacctgac aggtcgcggg gtggccggcg ggtttttgcg
     3181 cttcaggtcg ccgcgccggg tctataggat gggttggcac cactgtcgat cacgatgtga
     3241 ggaatcctga ccagctccga gttctcggca ccgatccctg accgggcgtc cagcgcgtcg
     3301 gacaccgctc acgtcgaggg cttgcgtcgg gtcacggtcc cggcctcgat cgacatggtc
     3361 aacgtcctgg gaccgcgcga cgccttcctc cgcatcctgg agcgtgagct cgaggccgac
     3421 atccacgtcc gcggcaatga gatcaccctg agcggaccgc cgcacgcgct cgcgctgggc
     3481 gtcgaggtca tcaacgagct catcaccgtg ctccgcaccg gccagggcct gaccggcgac
     3541 gccgtcgagc gcatctgcag catgctcgcg gcccgtgaag aggcccggcc ggcggacgtg
     3601 ctcacccaca acatcttgtc cagccggggc cggaccatcc ggccgaagac cctcaaccag
     3661 aagcggtacg tcgacgccat cgacaagcac accgtcgtct tcggcatcgg tccggcgggg
     3721 acgggcaaga cctatctggc gatggccaag gcggtgcagg cgctgcagaa caagcaggtc
     3781 aaccggatca tcctcacccg tccggcagtc gaagccgggg agcacctggg cttcctgcca
     3841 ggcaccctca acgacaagat cgacccctac ctgcgcccgc tgtacgacgc cctgcacgac
     3901 atggtggatc ccgagtcgat cccgcggttg ctgactgccg ggaccatcga ggtcgcgccg
     3961 ctggccttca tgcgcggccg cgctcagccg gtgagcacgc cggtgttgac tcccgatggc
     4021 ttccgaccaa tcggcgatct tcgggtcggc gatctcgtgg tcgggtcgaa cggggagccg
     4081 acgccggtgc tggggaccta tccgcagggc cgcaaggagg tctaccgcgt ctctactcag
     4141 gacggcgcgt cgaccctagt ctgcggggag cacctctggt tcgtgacgac gccgtccgat
     4201 cgacgacacg gcaagactgg ccgaatcgtg cagacccagg acatgatcgg caagctgcgc
     4261 gtcgctcatc agcgccgctt cgagttgccg atggtggcgc cggtccggat gcccgagcga
     4321 gaggttccga tcgatcccta cgccttgggc ctgctcctgg gcgacggctg catcaccacc
     4381 aagacgacac ccagcttctg cacgcgcgat cccgaactgg tcgcgtcgct cgaggcggct
     4441 ctcccggaca tcgagctggt caaaaaatcc gagatcgact acgtgcttcg ccatctgaac
     4501 ggtcatcgcg gtggagtgat cgtctccaat cccgtcacgg tggctcttcg tgagctccag
     4561 ctcgccggca ccgactcgca cacgaagttc ataccgcagg gatacctggt caacagcgca
     4621 gcggttcggc tggcggtgtt gcagggcttg ctggacaccg acgggggacc ggtccgccag
     4681 acggcacgta cttgtcgcgt gcagtacacg acatgctccg atcggcttcg cgacgatgtc
     4741 atcttccttg ttcgctcgct gggcggcgtg gcctacacgc ggacgcgttt cgccgccgga
     4801 cgcaagccgg gcctggcgaa cggcaagccg gtcccgtacc gccacgacgc gtacgtggtc
     4861 gacattcggc tgcccttcgg agtccccccg ttccggctgc agcgaaaggc cgacctctat
     4921 cagcgagagg gcggtggcgg acgaccgatg cggttcgtcg attcgatcga acccgccggt
     4981 gaggccgaat gcgtgtgcat ctccgtggca gcagaggatt cgctgtatgt caccgaggac
     5041 tttctggtta cgcacaacac gctcaacgac tccttcatca tcctcgacga ggcgcagaac
     5101 acctcgatgg agcagatgaa gatgttcctg acccggctgg gcttcgggtc caagatcgtg
     5161 gtcaccggcg acatcacgca ggtcgacctg ccgggcggca tgcacagcgg tctgcgggtg
     5221 gtccagggca tcctcgacga ggtggacgac atctcgttct gttacctgac caaccacgac
     5281 gtcgtccggc acaagctggt cgggcggatc gtcgccgcgt acgaccggtt cgagg
//
LOCUS       DATBFZ010000443         1667 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_4751492, whole genome shotgun sequence.
ACCESSION   DATBFZ010000443 DATBFZ010000000
VERSION     DATBFZ010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4751492"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1017)
                     /locus_tag="VH419_13510"
     CDS             complement(<1..1017)
                     /locus_tag="VH419_13510"
                     /inference="COORDINATES: protein motif:HMM:NF014148.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HEY7044680.1"
                     /translation="MPKVAFIGAGSVEFTRNVVTDLCSYPELHGTLELALHDIDAERL
                     GHAETMARRIVAQTDAGATVTANLDRVAAIAGADYVVNEIQVGGYPATRIDFDIPAGY
                     GVRQTIADTIGIGGIFRGLRTMPVLIGIGEDMAKVCPDAYLLNYTNPMAMLPWAVYAA
                     TSFDRVFGLCHSVRDTHGFLAGLVGLPADQIDFTTTGFNHQAFVLRFEHQGKDLYPQL
                     REAIEADPDLQRRVRVEVFRRFGYFPTESSEHSAEYLPWFMRHDGEIEHFRIFVGDYL
                     HRSEENIDEYEQTRRELESDAPLQLQATSELASEFIHAHQTGTAREIYVNVRNGGKVT
                     SLPDD"
     gene            complement(1058..>1667)
                     /locus_tag="VH419_13515"
     CDS             complement(1058..>1667)
                     /locus_tag="VH419_13515"
                     /inference="COORDINATES: protein motif:HMM:NF012669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HEY7044681.1"
                     /translation="HGGAVASGVGLELPLRHRAARRQPQKGAIGRMAATLVPEGAVVG
                     MTGGTTVTEVARALKSSDGITVVTNALNIAAELVLSPSPRVVVVGGAARHASYELVGP
                     ASEMIIERYHLDISFIGVDGVTLEEGCSTYDEMEAHTDHAFIKRARRTIVVADSTKLG
                     KRTFARICRTDEISDFVTDADADAGFLDGLRDVGVNVLIAGN"
BASE COUNT          254 a          552 c          560 g          301 t
ORIGIN      
        1 gtcatccggc aggctggtca ccttgccgcc gttgcggacg ttcacataga tctcgcgagc
       61 ggtgccggtc tggtgcgcgt gaatgaactc cgaggccagc tcggacgtgg cttgcaactg
      121 cagcggcgcg tcgctctcca gttcgcgccg ggtctgctcg tactcgtcga tgttctcctc
      181 ggagcggtgc aagtagtcgc cgacgaagat ccggaagtgt tcgatctcgc cgtcgtggcg
      241 catgaaccac ggcaggtact cggcggagtg ttcgctcgac tcggtcggga agtatccgaa
      301 tcggcggaag acctcgacgc gcacccgccg ctgcaggtcc gggtctgcct cgatcgcctc
      361 gcgcagctgc gggtagagat ccttgccttg gtgctcgaac cgcagcacga atgcctggtg
      421 gttgaacccg gtggtggtga agtcgatctg gtcggcgggc aggccgacga ggccggcgag
      481 gaagccgtgc gtgtcgcgta cggagtggca caacccgaac acccggtcga acgaggtcgc
      541 cgcatagacc gcccacggca gcatcgccat cgggttggtg tagttcagca ggtaggcgtc
      601 ggggcaaacc ttggccatgt cctctccgat gccgatcaac accggcatgg tgcgcaggcc
      661 gcggaagatt ccgccgatgc cgatggtgtc ggcgatcgtc tgccgcacgc cgtagccggc
      721 agggatgtcg aagtcgatcc gggtggccgg atagccgccg acttggatct cgttcacgac
      781 gtagtcagcg cccgcgatcg cagcgacgcg gtcgaggttc gcggtgacgg tagcgccggc
      841 gtcagtctgg gccacgatgc gccgcgccat cgtctcggcg tggccgagcc gctccgcgtc
      901 gatgtcgtgc agggcaagtt cgagcgtgcc gtgcagttcc gggtagctac acagatcggt
      961 gacgacgttg cgggtgaact cgacgctgcc tgcgccgatg aacgcgacct tcggcatgtg
     1021 caaacctttc tcggtaggga cgctcgtcgt tctgggttca gttgccggct atcaggacgt
     1081 tcacgcccac gtcgcgcagc ccgtcgagga agccggcatc ggcatccgcg tcggtgacga
     1141 agtcgctgat ctcgtccgtc cggcagatcc gcgcgaaggt gcgcttgccg agcttggtgc
     1201 tgtcggcgac gacgatggtg cgccgcgccc gcttgatgaa cgcgtggtcg gtgtgcgctt
     1261 ccatctcgtc gtacgtgctg cagccctcct cgagcgtcac cccgtcgacg ccgatgaacg
     1321 agatgtccag gtggtaacgc tcgatgatca tttccgatgc cgggcccacc agttcatagc
     1381 tcgcgtgtcg cgccgcgccg cccaccacga cgacgcgcgg cgacggactc aacaccaatt
     1441 cggccgcaat gttcaacgcg ttggtgacga ccgtgatgcc gtcgctgctc ttgagcgccc
     1501 gagccacttc ggtgactgtc gtaccaccgg tcatcccgac cacggcaccc tcgggcacaa
     1561 gcgtcgccgc catccgtccg atcgccccct tctgcggctg gcgccgtgcc gcgcggtgcc
     1621 gcagcggaag ctcgagcccc accccgctcg cgacggcgcc gccatgc
//
LOCUS       DATBFZ010000444         2308 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_5082898, whole genome shotgun sequence.
ACCESSION   DATBFZ010000444 DATBFZ010000000
VERSION     DATBFZ010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5082898"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(370..1545)
                     /locus_tag="VH419_13520"
     CDS             complement(370..1545)
                     /locus_tag="VH419_13520"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR04350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004121 - cystathionine beta-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HEY7044682.1"
                     /translation="MASIERSVEDMRARRGTKWRKFPPDVLPAWVADMDFSVPDEVQA
                     AVDRITTLGDYGYAAREGDESLAAAFQFYAKQSFGWDVDPDGVLPVGDLIQGMYSSVY
                     AFSEPGDGIVVQTPIYPPFLDTIATTERRLVENRLLDDGTKYAVDTDGLRKVVDDRTR
                     LLMIPNPHNPTGRTFSRDELQVMLDVAIEHDLIVVSDEIHADLVYSGNKHIPFASLGP
                     EAAARTITLTSATKGFNIPGLRCALMYFGSQDLKARFHKRMPQRLLGSPNVIGIDATV
                     AAWRYGQPWLTQVLAILEGNRTRVTEFLATQMPAVTYRQPQATYLAWLDCRALNLGAP
                     PFDYFLEKAKVGLNDGAAFGEAGVGCVRLNFGTSPKILDEILGRLAESVREVALAPA"
     gene            1700..>2308
                     /locus_tag="VH419_13525"
     CDS             1700..>2308
                     /locus_tag="VH419_13525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044683.1"
                     /translation="MTPARPGLEAAVSLRLLAVGVALWVAAVSMTSGVATAQSTDPMD
                     IVTFHEEAGKQAQTVADEAGADQYGDWAHRRWTRDIDNSDKYVGPVVTDSKVWVAKDV
                     DAAQKLYKLQADQNAKMPERDIDANGPFPWVIKEGPQPKDFADEGTGASACRHNNCDE
                     PGKVFTHRRVVIRVGRYVATVYLYGRDETATPELATWFARKMS"
BASE COUNT          379 a          796 c          762 g          371 t
ORIGIN      
        1 cctccagcta catatctcca gatccgggta cccccggtcc gctgccgctc gacagatcgc
       61 gtcggaccgt tcgtgcgacc tcccggcatg acgcgcgcca gcatgctgga cgcgtggtcc
      121 ggtgagtctc gcgcttgtcg ctgccggctc gcgttccgtt tgcttttacg cccgccgatt
      181 gcgcccggct ttggagcgtg cgcagcaggt attcccggcc gatgttccca aaacccgacc
      241 tccttgtcga gaggccgggc tccaggagca ctccaggagc acgcccatac ctgtgagacc
      301 gtggccgggt gtcattgcag gatagaacgg gaactcgaaa tcccgggggc tcccgtcgct
      361 tcgcccgcct caggccggcg ccagcgccac ctcccgtacc gattctgcga gccggccgag
      421 gatctcgtcg aggatcttcg gcgaggtccc gaagttcagc cggacgcagc cgaccccggc
      481 ctccccgaac gccgcaccgt cgttcagccc gaccttcgcc ttctccagga agtagtcgaa
      541 cggcggggcg ccgaggttca gcgcgcggca gtcgagccag gccaggtagg tcgcctgggg
      601 ctgccgatac gtgacggccg gcatctgggt cgccaggaat tcggtcacgc gggttcggtt
      661 cccctccagg atcgccaaca cctgcgtcag ccagggctgg ccgtaccgcc aggccgccac
      721 cgtcgcgtcg atgccgatca cgttcggcga gccgagcagc cgctgcggca tccgcttgtg
      781 gaaccgcgcc ttcagatcct gcgacccgaa gtacatcagt gcgcagcgca acccgggaat
      841 gttgaacccc ttggtggccg aggtgagcgt gatggtgcgg gccgccgcct ccggcccgag
      901 cgaggcgaac gggatgtgct tgttgcccga gtagacgaga tccgcgtgga tctcatccga
      961 gaccacgatc aggtcgtgct cgatcgcaac gtccagcatc acctggagct cgtcgcggct
     1021 gaaggtgcga ccggtcgggt tatgggggtt cggaatcatc agcagccgcg tgcggtcatc
     1081 gacgaccttc cgcagaccat ccgtgtcgac ggcgtacttg gtgccgtcgt ccagcagccg
     1141 gttctcgacc agccggcgct cggtcgtcgc gatcgtgtcc aggaacggcg ggtagatcgg
     1201 cgtctggacg acgatgccat cgcccggctc gctgaacgcg tacaccgacg agtacatccc
     1261 ctgaatcagg tcgccgaccg gcaacacgcc atcgggatcg acgtcccagc cgaacgattg
     1321 cttcgcgtag aactggaacg cagcggccag gctctcgtcg ccctcgcgcg cggcgtaccc
     1381 atagtccccg agggtcgtga tccggtcgac ggccgcctgg acctcgtccg ggactgagaa
     1441 gtccatatcc gcgacccagg ccggcagcac gtcgggcggg aactttcgcc acttcgtgcc
     1501 ccgtcgcgcc cgcatgtcct cgactgagcg ctcgatgctc gccaccgttg tcacctccgg
     1561 acagacctag cgcttccgat ggtagccggt ccgccgaccg gcgtgcggtg tgtcccttgc
     1621 aacgcatggg tggcacgttt cgcatccgat cgggaaccat ggacgttatg aggagtgaca
     1681 tcgcggcaag ggagcggctg tgacacctgc tcgaccaggg ctcgaggcgg ccgtctcatt
     1741 gcggctgctg gcggttggcg tggcgctgtg ggtggcggcc gtctcgatga cctccggcgt
     1801 cgccaccgcc cagagcaccg atccgatgga catcgtgacg ttccacgagg aggccggcaa
     1861 gcaggcccag accgtggcgg acgaggccgg cgcagaccag tacggcgact gggcccatcg
     1921 ccgctggacc cgtgacatcg acaactccga caagtacgtc gggccggtcg tcaccgacag
     1981 caaggtctgg gtggcgaagg acgtcgacgc cgcccagaag ctgtacaagc tccaggccga
     2041 ccagaacgcg aagatgccgg agcgcgacat cgacgccaac ggtccgttcc cgtgggtgat
     2101 caaggaaggt ccgcagccga aggacttcgc cgacgagggc accggcgcca gcgcctgccg
     2161 ccacaacaac tgcgacgagc cgggcaaggt gttcacgcac cgccgggtag tgatccgcgt
     2221 cggccgctac gtcgcgacgg tctacctgta cggccgtgac gagacggcca cgccggagct
     2281 ggcgacctgg ttcgcgcgca agatgtcc
//
LOCUS       DATBFZ010000445         5769 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_972385, whole genome shotgun sequence.
ACCESSION   DATBFZ010000445 DATBFZ010000000
VERSION     DATBFZ010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 5769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5769
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_972385"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1535
                     /locus_tag="VH419_13530"
     CDS             <1..1535
                     /locus_tag="VH419_13530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA helicase"
                     /protein_id="HEY7044684.1"
                     /translation="VTPGEYTQLTGRAGRRGIDVEGHAVVLWAPGFDPKAVAGLASTR
                     TYPLRSSFRPSYNMAVNLVRQVGRDTARELLESSFAQFQADRAVVGLARQVRKAEEAL
                     AGYADAISCDRGDFMEYAGLRRALSDREAALSKRRKADRRDAAQESLETLRIGDVIEV
                     PAGRWSGIAVVIDPGMRSQREGPRPLVLTVDRQARRLSMVDFPTPVEPLTRLRIAKTF
                     NPRNAQQRRDLAALLRDRTRDLPRSDGSGSRRGRRDQSSAAEDPEVQRLRDAIRRHPC
                     HGCPDREDHARWAERYLKLGRETETLKRRIEQRTNTIARQFDRVCEVLEELDYLEPAS
                     GDGGDAVTEAGARLARIYSELDLVAAECLRRDVWTGLEAPGLAAALSALVYESRNPDD
                     ADRPRLPGGAVRTALGDTVNIWAELDLVERDHRLSFLREPDLGFAWAAYRWAGGAELD
                     EVLDEVDLAPGDFVRWVKQLLDLAEQIADAAGDMPLRRTAREAVRSMRRGVVAYSTGL
                     DM"
     gene            complement(1938..2879)
                     /locus_tag="VH419_13535"
     CDS             complement(1938..2879)
                     /locus_tag="VH419_13535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease"
                     /protein_id="HEY7044685.1"
                     /translation="MTDGSSRLMLLDTASLYFRAFFGVPDTVRAPDGTPVNAVRGLLD
                     FIARLVTTGRPTHLVCCWDNDWRPQWRVELISSYKAHRVEYVVADGVDVEETPDPLEA
                     QIPMIEAVLEALGIAVVGADGYEADDVIGSLASQTPGMVDVVTGDRDLFQLVDDEQQI
                     RVLYIARGVGNLETLTDATIVGKYGILPQQYVDFAVMRGDASDGLPGVKGVGEKTAAG
                     LLQQYGDLAAIREAAADPTSGLATGVRSKILAAADYLDVAPKVVAVARDLKLPTVDSR
                     IKPIGEESEEVLTTLQETWGLGGSVTRVVEAFAATSA"
     gene            3067..4179
                     /locus_tag="VH419_13540"
     CDS             3067..4179
                     /locus_tag="VH419_13540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009078205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEY7044686.1"
                     /translation="MTADISSRIRRVQEAMSAADVSGLMVTPGPDLRYLSGYDAKPLE
                     RLTALVVPVDGDPALVVPALELAEAQRSPAALSGMEVVTHGETDDAYALATSLFKEGS
                     GTIAVDDHMWSVRVFALSDALPSTRLIAAGRLIGVLRMRKGADEVASLRQAGQAIDRV
                     HQRMAEFLHVGRSERDAARLIADAIVAEGHATADFVIVGSGPNSASPHHSVSERVIEA
                     GDSVVIDIGGTTADGYCSDSTRTYVVGGEPSPDVCEYFDVLLEAQRTQCDYAKPGVTA
                     ESVDRTGRQIIADAGFGEYFMHRTGHGIGLETHEEPYIVEGNDLVLEPGMTFSIEPGI
                     YLTGKHGARIEDIVVTTEDGADRLNTTNRELTVLPG"
     gene            4203..4643
                     /locus_tag="VH419_13545"
     CDS             4203..4643
                     /locus_tag="VH419_13545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006141531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEY7044687.1"
                     /translation="MEDVDRLICRLLAADGRMSFTDLGKAAGLSTSAVHQRVKRLESR
                     GFIKGYAALVDYDLVGRPLTAFISIRPIDPSQPDDSPERLADIPEIEACHSVAGDESY
                     ILKVRVAEPIDLEDLLARIRARANVSTRTTIVLSTPYEHRPPHL"
     gene            complement(5475..>5769)
                     /locus_tag="VH419_13550"
     CDS             complement(5475..>5769)
                     /locus_tag="VH419_13550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012921218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysine 2,3-aminomutase"
                     /protein_id="HEY7044688.1"
                     /translation="RIVCDVPFVGKRWVHQLESYDREHGISYWTKNYRTSIERTDPDA
                     LTRTYEYYDPIHTLPQAGQDWWREHAGDVLAAAEEAAKASREAAESQKSLPLV"
BASE COUNT          899 a         1959 c         1969 g          942 t
ORIGIN      
        1 acgtgacgcc gggggagtac acgcagctga ccggcagagc tggtcgtcgc ggcatcgacg
       61 tcgagggaca cgccgtcgtc ctctgggcgc cgggcttcga cccgaaggcg gtggccgggc
      121 tcgcctccac tcgtacctat ccgcttcgct cgtcgttccg gccgtcgtac aacatggccg
      181 tcaacctggt gcggcaggtc gggcgggaca ctgcgcgcga gctgctcgag tcctccttcg
      241 cgcagttcca ggcagaccgg gcggtcgtcg gcctcgcccg gcaggtgcgc aaggcggagg
      301 aggcgctggc cgggtacgcg gacgcgatca gctgcgatcg gggcgacttc atggagtatg
      361 ccgggcttcg ccgcgcactg tcggaccgag aggcggcgct gtccaagcga cgcaaggcgg
      421 accggcggga tgccgcgcag gagtcattgg agacgttgcg catcggcgac gtgatcgagg
      481 ttcccgccgg gcgctggtca ggcatcgcgg tcgtcatcga tccaggcatg cggtcgcagc
      541 gggaggggcc gcgtccgctg gtgctgaccg tcgaccggca ggctcgtcgc ttgtcgatgg
      601 tcgacttccc gactccggtg gagccgttga cccggttgcg gatcgccaag acgttcaatc
      661 cgcggaacgc acagcagcgg cgagacctgg cggcgttgct acgggaccgg acccgggacc
      721 tgccccgctc tgatggcagc ggcagccgga ggggtcgccg ggaccagtcg tcggcggccg
      781 aggacccaga ggtgcagcgg ctgcgagacg cgatccgccg tcatccgtgc cacggctgtc
      841 cggatcggga ggaccatgcc cgctgggccg agcgctatct caagctcggg cgcgagaccg
      901 agacgctgaa gcggcggatc gagcagcgca cgaacacgat cgcccgacag ttcgaccggg
      961 tctgtgaggt gctggaagag ctcgactacc tcgagcccgc ctccggcgac gggggtgacg
     1021 ccgtcaccga ggccggcgcg cggttggcgc ggatctacag cgagctcgac ctcgtcgcgg
     1081 ccgaatgcct gcgccgcgat gtctggaccg ggttggaggc accgggattg gcggcggcgc
     1141 tgtcagccct ggtgtacgag tcgcgcaatc ccgacgatgc cgaccggccg cgccttccag
     1201 ggggtgcggt ccggacggcg ttgggcgaca ccgtgaacat ctgggcggaa ctcgacctcg
     1261 tcgagcgtga ccatcggctg agcttcctgc gcgagcctga tctcgggttc gcctgggcgg
     1321 cgtaccgctg ggccggcggt gctgagctgg acgaggtgct cgacgaggtc gaccttgccc
     1381 cgggcgactt cgtccggtgg gtcaagcagc tgttggacct ggcggagcag atcgccgacg
     1441 cggccggcga catgccgctg cgccgaaccg ctcgggaggc cgtcaggtcc atgcgccgcg
     1501 gcgtcgtcgc ctactccacg ggcctcgaca tgtagcccgc tcccgctgag ctggtcggcc
     1561 ggctttggga ctcctcaggt gcgtggcgtg gagacctcgc cattggtggg cgtggtcgcc
     1621 ctggcgcgcc cgtggtcacg ggccgaggcg tttcggcagt gaagagcgga aacgttcgtg
     1681 cccgctgagc cggccggctg gctttccgtc ccagttgggc ggtgtgtgac tggcggtttc
     1741 ggacatcggc cggtcagcga gccggtttgc cacggtcacc atgcaaaccc acccgtcccg
     1801 aggcagatct gcctggctcc ccgtccgttt ggatggctac ccaggcaaac caccaccgac
     1861 accgtttcca ttggtgcggt agggggccaa ccggaagatc gctgggccaa acgcccgagg
     1921 gcgactggat gtgatggtca ggcggacgtg gccgcgaacg cctcgacgac gcgagtgacc
     1981 gagccgccga ggccccacgt ctcttgcagc gtcgtgagca cctcttcgga ctcctcaccg
     2041 atgggcttga tccggctgtc gacggtcggc agcttcaggt cacgcgcgac ggccacgacc
     2101 ttcggagcca cgtcgagata gtcggccgcc gccaggatct tgctgcgtac accggtcgcg
     2161 agcccacttg tcggatcagc ggcggcttcc cggatcgcag ccaggtcgcc gtactgctgg
     2221 agcagccctg ccgcggtctt ctcgcccacc cccttgacac cgggcagccc gtcggacgcg
     2281 tccccacgca tcaccgcgaa gtccacgtac tgctgcggca ggatcccgta cttgcccacg
     2341 atcgtggcgt cggtcagcgt ctcgaggttg ccgaccccgc gtgcgatgta gaggacccgg
     2401 atctgctgct cgtcgtcgac aagctggaac aggtcacgat cgccggtcac gacgtcgacc
     2461 atccccggag tctgcgacgc cagcgagccg atcacgtcgt ctgcctcgta cccgtcggcg
     2521 ccgacgaccg cgatcccgag cgcctcgagg acggcctcga tcatcgggat ctgcgcctcg
     2581 agggggtccg gcgtctcctc gacgtcgacc ccatcggcca cgacgtactc cacgcggtga
     2641 gccttgtagc tcgagatcag ctcgacccgc cattgcggtc gccagtcgtt gtcccagcag
     2701 cagacgaggt gcgtcggtcg cccggtggtg accagccggg cgatgaagtc gaggaggcca
     2761 cgaacggcgt tcaccggcgt accgtcgggc gcccgcaccg tgtccggtac gccgaagaac
     2821 gcccggaagt acagggacgc ggtgtcgagc aacatcagcc gcgagctgcc gtcggtcatg
     2881 gcgtcactct aggatctgcg ttccgaggca cgatcctcac ccatgcgtga cgtgcctatg
     2941 aacactgaag agccttgtac ggtgacggct cggctgcacc gtggatcgca acgccgcgaa
     3001 cgcctcctcg gagcgcgtcc gagtcggacg aatcgggatc ccgttggcgc cactaggctc
     3061 taggtcatga ctgccgacat ctcctcacgc atccgtcggg tgcaggaagc gatgtccgcc
     3121 gcggacgtgt ccggcctgat ggtcacgccc ggaccggacc tgcgctacct gtcgggctac
     3181 gacgccaagc cactcgagcg gctcaccgcc ctcgtcgtac ccgtcgacgg tgacccggca
     3241 ctcgtcgtac ccgccctcga gctcgctgag gcacagcggt cgccggccgc cctgtccggc
     3301 atggaggtgg tcacacacgg ggagaccgac gatgcgtacg cgctcgccac gtcgttgttc
     3361 aaggagggaa gcgggaccat cgcggtcgat gaccacatgt ggagtgtccg ggtgttcgcg
     3421 ctctccgacg cgttgccctc gacgcggctg atcgcagccg gccggctgat cggtgtcctg
     3481 cggatgcgca agggagccga tgaggtcgcc tcgcttcggc aggctggcca ggcgatcgac
     3541 cgcgtccacc agcggatggc ggagttcctc cacgtcggca ggtccgaacg tgacgccgcc
     3601 cggctcatcg ccgacgccat cgtcgccgag gggcacgcca ccgccgattt cgtgatcgtc
     3661 ggctcgggac cgaacagcgc ctcgcctcac cactcggtct ccgagcgcgt gatcgaagcc
     3721 ggagactccg tggtgatcga catcggcggt acgacggcgg acggctactg ctcggactcg
     3781 acccgcacct acgtggtcgg tggtgaaccc agcccggatg tctgtgagta cttcgacgtt
     3841 ctgctggagg ctcagcggac gcagtgcgac tacgcgaagc ctggggtgac ggcagagtcc
     3901 gtcgaccgga cagggcggca gatcatcgcc gacgccggct tcggtgagta cttcatgcac
     3961 cgcaccgggc atggcatcgg cctcgagacg cacgaggagc cttacatcgt ggaggggaac
     4021 gacctcgtcc tcgagccggg gatgacgttc tccatagagc ccggcatcta cctgaccggc
     4081 aagcacggcg cccggatcga ggacatcgtc gtcacgaccg aagacggcgc cgatcgcctc
     4141 aacacgacca accgcgagct cacggtcctt cctgggtagc gccgatgcca catgatcgtt
     4201 cgatcgagga cgtcgaccgg ctgatctgcc ggttgctcgc cgccgacggc cggatgtcct
     4261 tcaccgatct cgggaaggcc gccgggttgt caacgtcggc ggtgcaccag cgggtcaagc
     4321 ggctggagtc gcgcgggttc atcaaggggt acgccgccct ggtcgactac gacctcgtcg
     4381 gccgtccgct caccgcgttc atctcgatcc ggccgatcga cccgtcccag ccggacgact
     4441 caccggagcg ccttgccgac atcccggaga tcgaggcctg ccactccgtc gccggtgacg
     4501 agtcatacat cctcaaggtc cgggtcgccg agccgatcga cctcgaagac ctgctcgctc
     4561 gcatccgggc ccgcgccaac gtctccaccc gcacgacgat cgtgctcagc acgccgtacg
     4621 agcaccgccc accgcatctg tgaggcggtc ccgggggtga cggtttgcct ggtgagccat
     4681 ccgaatgcac ggctgaccag gcagatctgg gttctcctcg gcttcgctgg gtggtgatgg
     4741 tcgccatggg ctgcgtggtc gcgcgagagg cactgtcgtg atgggctgag gtggatcggc
     4801 gatgaagagc ggcgaccttc gtgccttctg agttggctgg ccggctttcc ggcccctgtg
     4861 gttggtgtct gactggcggt tttcggacat tcgcccgttc agcgccccga tctgcctcgg
     4921 tcaccaggca gacccacccc tggcaaggca gatctgcctg gctatccgtc catttgcacg
     4981 gctaccgagg caaaccacca gggacgcctg tccgatcggt tccgtaggtg gcttgccggt
     5041 agcaagcgca gcccacaaga acccgagtat gccgcctacc gcacaaatcg gtcacgtgct
     5101 gcgggatggt ttgcgatgtt taccgcccaa gcggcgtgta accatcgcag acctgaggtg
     5161 gacagagtgg ggttgggatg tggacatcgc aaaccggggg cactgaccgg ccgatgtccg
     5221 aaacccgcta ctcagacgcc gacaaaaagg gacggaaagg cggccggtca gctccgcggg
     5281 cacgaacgtt tcccgctctt cattgccgaa accgcctcag cccataacga cgcagcgtcg
     5341 ggcgtcgggc gtcacgcgtc acccatcgcg accatgtccg cgcacaacgc cccgaagagg
     5401 acccaaacct gccttggccg cctcgaccgc ggcccttggt cgcctcggcc agcgcacgtg
     5461 gtgacttcga cgactcagac gagaggcagg gacttctggg actcggcggc ctcgcgggac
     5521 gcttttgcgg cctcctcggc ggcagccagc acgtcgcccg cgtgctcccg ccaccagtcc
     5581 tggccggcct gcgggagcgt gtggatcggg tcgtagtact cgtacgtccg ggtcaacgcg
     5641 tcagggtcag tacgctcgat cgaggtccgg tagttcttgg tccagtacga gatcccgtgc
     5701 tcacggtcgt atgactcgag ctggtgcacc cagcgcttgc cgacgaacgg aacgtcacac
     5761 acgatgcgc
//
LOCUS       DATBFZ010000446        14486 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_3268033, whole genome shotgun sequence.
ACCESSION   DATBFZ010000446 DATBFZ010000000
VERSION     DATBFZ010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 14486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14486
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3268033"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..610)
                     /locus_tag="VH419_13555"
     CDS             complement(<1..610)
                     /locus_tag="VH419_13555"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY7044689.1"
                     /translation="MKARQPDSSGYAVNDGVRVYYEVHGTGSTTVVLLPTWAIVDSQV
                     WKMQVPYLSRYFRVLTYDPRGNGKTDRPQDPAAYTYELASEDVEAVMDATDTEQAVLV
                     AYCDGTAPAVLFSGRRPERVSGLAVFAPNTPAVAVVPERRRRNYDFESVVDEPVGWAK
                     ETRAYWQQNWAGYLDWFFANVASEPHSTKVYDDLVDWGLQTDA"
     gene            complement(778..2193)
                     /locus_tag="VH419_13560"
     CDS             complement(778..2193)
                     /locus_tag="VH419_13560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY7044690.1"
                     /translation="MSTVIDVDTAREQLTDFSGELIGRSDASYDEAQKVFNAYIQKRP
                     LLVARPANPADVATAIAFARRHELPLAVRGGGHNGAGLGTCDDGVVIDLSKFRDIEVN
                     ADARTVTVGGGCRWGEVDQATNAFGMATPSGIISTTGVGGLTLGGGLGHLTRRCGLSI
                     DNVLGAEMVLADGRHVTVNAEQEPDLYWAIRGGGGNFGVVTSFTFRLHDVSTVIGGPT
                     FWPVEQTEEVLSAYREFLPNAPRELNGFFALHIVPPGDPFPEAIHLRKVCGVVWCFTG
                     DDEAAAARAMAPLLDSLPEPLLHGVAPMPHPVLQGAFDALYPAGDQWYWRADFVNQVP
                     DDAVRIHSQFGASIPTTKSTMHMYPVDGAAHDVRADETAWSYRDATWGSVYAGVDPDP
                     ANAGKIKQWTVDYFDALHAYSAGGAYVNMMMDEGQDRVMASYRDNYPRLAAIKAAYDP
                     ENVFSINQNVRPQQPVPEQRP"
     gene            complement(2323..4641)
                     /locus_tag="VH419_13565"
     CDS             complement(2323..4641)
                     /locus_tag="VH419_13565"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEY7044691.1"
                     /translation="MKRVRPVGGDLIGRDAELARTTALLDGDVRLLTLAGPPGVGKTR
                     LAQELGSRCADRYADGAVFVDLSATRDQSGAMNEIARGLGIDSRPGEDGVVRVRQWLM
                     DRDMLVILDNAEQVTGLPSELSALLRSTSRLTILVTSRENLHLSQEHQFPVPPLAMPV
                     ADDVADLARFQAIPSVALFTAAARAVRPDFHVSPDNANAVAEICVRLDGLPLALTLAA
                     PRVKVFSPADIAARLRDRRALLEATARDVPARHRSLRAAISWSHDVLTEDERRLFRRL
                     SVFASPWTLEVATQVCGGDGLDPDAVEHTMLSLVDKSLVQPADVEDAEQHFSMLQSVR
                     DFATEQRKRYDDGSLAEARHSAYFVGQALRSEASLGTPEEPRWYDWATPHERDVRTAL
                     DRVLAHNDLEQALPLVTALGWSSYTHGYVGAGKALVDEVLARVDGAAEPVDPSLEGPA
                     RAIGGVLAWSVGDLDLAHGQLERALDSVRRTGNLRRMALVHAFLGHVARDRGDFVEAA
                     AQHREAARLYDELGHDLGSAWARFDLGRVTWQRGEPSAASPLMADALDRFRRLDYVWA
                     VAWTSWALGTLLAEGGTIDDGAGFLAGSLREFERASDLRGAALCWESLATVAVAVDRP
                     GDAVRLATAAAAVRDRLAAPRVGTELARVEASAVAARAALGDYGFDRARQDGMTLPVA
                     SGYELAYEVAAAAARSTATPTTPQWETLTVREREVAGLVADGCTNQQIGRRLGIAART
                     AEAHVHNIMAKLDARNRAEVAAWSATQRPADR"
     gene            complement(4760..5845)
                     /locus_tag="VH419_13570"
     CDS             complement(4760..5845)
                     /locus_tag="VH419_13570"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY7044692.1"
                     /translation="MNGSTYDVVVVGGRVAGSLTAILLARAGHRVLVIDRARFPSTTL
                     STHFFRGNGLIRVLDMAGMLDAVLALGPPRLTCEYAYFNGDPSPTIEPPQDPGDAGYC
                     LSVRRETLDAVLLDGARRAGAHVRTATSVSRLITEGERVTGVLLGDGSTVMAGLVVGA
                     DGRRSMVARAVNAADRERHRGKRALYYRYVADMPSPSGGAPDGPEFSLLDDELAYIFP
                     SDAGLTCVAISVNLAAYARFRHSSAQVFDELLARHTGLWQRYADSTRHGRLFGSGPLD
                     DYVRQPAGPGWALVGDSGIHQDPWSGVGMDSAAVTAAALVECITSNADLRQNGWQVDY
                     ERNRDENVLSWFHDTVACAGDLSVLAS"
     gene            complement(5842..6417)
                     /locus_tag="VH419_13575"
     CDS             complement(5842..6417)
                     /locus_tag="VH419_13575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044693.1"
                     /translation="MPDDSGDAKDRAATYDADALRQDLNTEYYAIVRIVGEFDGRLLT
                     MKSWSVTLSLAAIGLGFQQSHYALFALAAGSAVAFWLVEAITKRHQMRYYPRMREIEV
                     DTFGLNRLQVDGVDASAPRIDWAWTDAGRAGHHKSRARPTVLTDGEITRMLHHTFWMP
                     HVCIPHAVAVVVGVALFAMGAATPWLNDIPL"
     gene            6667..9900
                     /locus_tag="VH419_13580"
     CDS             6667..9900
                     /locus_tag="VH419_13580"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEY7044694.1"
                     /translation="MIFLWVVAGAVVGLVVARFGTETSNDLTLPGTGSQQVQDLLSDR
                     FPPQQNGTNPIVFDVAKGKLTDDANKQAINASIKAMRKAPNVYSVTNPISSDGQTAGL
                     LSKDGQTAFAPVLLDIGSGELDEEIAQQVFDTTHAAQEAGITVAAEGSIGSELSDQPS
                     ETSELVGIVAAMLILTLVLGSLVAMGLPIITAIVGLGIALACVGLLGHLVATPDTAAT
                     LATMIGLGVGIDYALFLITRHQDQLAAGVEMKESIANAVATSGSAIVFAGGTVVIALV
                     SLRVAGIPLLSTLGLASAVAVVTAVLAAISLLPALLGLLGHRIRWLSLPRFMRSKHRA
                     GRSRWTSWAGFVSRHPVWITLGSLALLLPLITPATSLNLGQEDIGAASPDTTERQAYD
                     LMTAGFGVGYNGPLQVASELDPPAQQSSEYTKKYDKATSLQKDLENKQKQLPKEQKHL
                     EQEQDQLERQKTSLQQQQAALTRRQKTLENKSARLEAQQSQLEAQQAQLLAQKQQLEQ
                     QQRELEAQKRQLQAQQRRLEAQQTRLEERRAELRAERKALAAEARPLLKQLARIIVHE
                     RILERRIARLDDRPRLQARLQARLDDLRQREQQVRRELAPLVRQAERLAQHARRLAAQ
                     AEELDAQAQRLQARADDLQSQGAALQQQADQLEAQGAALQRKADQLEAEGAALQRQAD
                     QLQAQGDQLEQQAAELQQQADQLQKQANQLKGEQQQAEQEQQQAEQLQKQLTTMLTKA
                     GGDPRGTDARVVRIQDDLAATSGILTLTPPQINKKGDVVLLSAVPTTAPATVATANLV
                     GTVRDDVVPTWEKAGGTTSYIGGYTASYVDLASLISQRLLLVIGTVILLGFLVLMVAF
                     RSLLVPLQASITNLLSAAASFGVLTAMFQWGWGTSLVGLDTTTPEVPIASYVPLMMFA
                     VLFGLSMDYEVFLVSHIQHHHRGGEPERKAVASGLASSGRITTAAALIMASVFASFIL
                     NGDPTIKQFGVGLSVAVLLAGALVVTLAPATLSLMGEAAWWLPGWLDRVLPHISVEGE
                     EQPPAPAPRPAAQATDTWQPAEYEAGASDIFPWRGDDLSDDGGATPR"
     gene            complement(9869..11392)
                     /locus_tag="VH419_13585"
     CDS             complement(9869..11392)
                     /locus_tag="VH419_13585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007079203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY7044695.1"
                     /translation="MTGDATRLTLVTGATGYVGGRLVPELLDHGHQVRVMARDPGRAA
                     ARPWASDVEIVAADALDREAVHAALDGVAVAYYLMHSIDARTSFGETERQIAMTFAEA
                     AADRGVRRIVYLGGMSPPGEELSAHLRSRAAVGDILLASGVPTAVLRAGVVIGSGSAS
                     FEMLRYLTERLPVMVVPRWVHTRVQPIAIRDVLHYLVKAAEMPDDVNRGFDIGGPDVL
                     TYLEMMQRYADVVGLPRRRMLAVPVLSPGLSSLWVGLVTPVPGRLARPLVESLRNTVV
                     AADKDIAAYVPDPSAGLTSYPRAVELALAKIQNMDVPTRWSSASMPGAPSEPLPSDPD
                     WAGGSLYVDEREIEVSATSTQLWSVVEGIGGERGWYSFRLGWAVRGLVDRLFGGPGLR
                     RGRRDRDRLLVGDSVDWWRVEALESGKRLLLRAEMRLPGLAWLEFEVAAVDATHSRLR
                     QRALFHPHGALGHAYWAAVYPFHGLVFATMQRNVAKAAESSACQPVGFNEGSLHRRR"
     gene            11607..12971
                     /locus_tag="VH419_13590"
     CDS             11607..12971
                     /locus_tag="VH419_13590"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: protein motif:HMM:NF015406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HEY7044696.1"
                     /translation="MAPAPAVAIALFTRNLRVHDNPVLVAASRHEHVVPLFVLDDGCR
                     PFVSANRAAFLVDSLEDLSSALRARGGALVVRRGKVVHEVCALARQTGATQVHVAADV
                     SRYAQRREHALAAALRAEGCELVVHEGVVTVVPPGAVTPQTKDHYAVFTPYFRRWLQQ
                     PRRPLLDPPRRFTLPRLHAGAIPRAADLCQGATSDRLMRGGERAGRARLTSWSRKVDA
                     YSERRDALSGDGTSRLSAYLHFGCVSPVEVIHRIGHDSAGRRDFVRQLAWRDFHHQLL
                     AARPECSWADYRPRGDQWRDDHAAFEAWRAGRTGIPVIDAAMRQLRTEGWLHNRARLL
                     VGSFLTKTLYIDWRWGARHFLDWLVDADIANNQLNWQWVAGTGADTRPNRVLNPVVQA
                     LRHDPDGRFVRRHLSELAGIAGPAVHQPWKLPPDVRRSLDYPDPIVDIDTAGRGFRAG
                     RAGR"
     gene            complement(13003..13347)
                     /locus_tag="VH419_13595"
     CDS             complement(13003..13347)
                     /locus_tag="VH419_13595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HEY7044697.1"
                     /translation="MAYDEELADRIRGLVAGEQGVSEQRMFGGLAFLINGNMSVAASG
                     QGGLLVRVDPEESEALVDGAHVEPMEMRGRQMSGWLRVETAAVMSPGQLEEWVARGVA
                     YAKSLPAKANRS"
     gene            13467..14147
                     /locus_tag="VH419_13600"
     CDS             13467..14147
                     /locus_tag="VH419_13600"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY7044698.1"
                     /translation="MNTPARYDGSAQWYDERFRHYGDLTEVDSSASLLATVLGRGDGW
                     CLDVGCGTGLHAAAIESTGRRVFGVDLSADQLAHAAHRIAVRVRTDATRLPFRDGTFP
                     SAVCTYVHTDIEQIGQVFAEVCRVMRHGGTFVYVGVHPCFWGPHIERVDETTRVVHPG
                     YWECSWRTRSPYWSDVGLASRVGFRHRTLADLLTELLSSGLRLTHVAEGARGAPFADR
                     IAIAAVKE"
BASE COUNT         2364 a         5010 c         4842 g         2270 t
ORIGIN      
        1 acgcgtcggt ctggagcccc cagtccacca ggtcgtcgta caccttcgtc gagtgcggct
       61 cgctcgcgac gtttgcgaag aaccagtcga ggtagcccgc ccagttctgc tgccagtacg
      121 cccgcgtctc cttcgcccat ccgaccggct cgtcgaccac actctcgaag tcgtagttgc
      181 gccgacgccg ttcgggcacg actgccactg ccggagtgtt gggggcgaag accgccaggc
      241 ccgacacccg ctcaggacgc ctgccggaga acagcaccgc cggcgccgtg ccgtcgcagt
      301 aggcgaccaa cacggcctgc tcggtgtccg tggcgtccat caccgcctcg acgtcctcac
      361 tggccagctc gtaggtgtac gccgccgggt cctgcggccg gtcggtcttc ccgttgccgc
      421 gggggtcgta cgtgaggacg cggaagtaac gggacaggta cggaacctgc atcttccata
      481 cctgcgagtc gacgatcgcc caggtgggca acagcaccac ggtggtgctg ccggttccgt
      541 gcacctcgta gtagacacgg accccgtcgt tgacggcgta ccccgagctg tcaggttggc
      601 gtgccttcat gcctgcggca cctccatcgg ccgatgcatc gtgagcgtag tcctgtggag
      661 gtttgcctgg tgagccatgc agatggctgg ttgaccaggc aaacctgcct cggttcgggt
      721 ggtgttgcct tgcgaggatc tccgcagcag cgttcgctga ccgtttcgcg gcacggccta
      781 cggtcgctgc tcaggcaccg gctgttgcgg ccggacgttc tggttgatgg agaacacgtt
      841 ctccgggtcg tacgccgcct tgatcgccgc cagtcgcggg tagttgtcgc ggtagctggc
      901 catcacccgg tcctggccct cgtccatcat catgttcacg taggcgcctc ctgcggagta
      961 cgcgtgcaac gcgtcgaagt agtcgaccgt ccactgcttg atcttgcccg cgttcgccgg
     1021 gtccgggtcg acaccggcgt acaccgatcc ccacgtcgcg tcacggtagc tccaggccgt
     1081 ctcgtcggcg cgaacgtcgt gcgcggcccc gtcgacgggg tacatgtgca tcgtcgactt
     1141 cgtcgtcggg atcgacgcgc cgaactggga gtggatacgc acggcgtcgt cgggcacctg
     1201 gttcacgaag tccgcgcgcc agtaccactg gtccccggcc gggtacagcg cgtcgaacgc
     1261 gccctgcagc accgggtgcg gcatcggagc gacgccgtgg agcagcggct ccggaaggct
     1321 gtcgagcagc ggtgccatgg cccgcgccgc agccgcctcg tcgtcgccgg tgaagcacca
     1381 cacgacgccg cagaccttgc gcaggtggat cgcctccggg aacgggtcac cgggcgggac
     1441 gatgtgcagt gcgaagaagc cgttgagctc gcgtggcgcg ttcggcagga actcccggta
     1501 cgccgagagc acctcctcgg tctgctcgac cggccagaag gtcggaccgc cgatcaccgt
     1561 gctcacgtcg tgcagccgga acgtgaagga cgtgaccaca ccgaagttcc cgccaccacc
     1621 ccggatcgcc cagtagagat cgggttcctg ctcggcgttg accgtgacgt gccgtccgtc
     1681 agcgaggacc atctcggctc cgagcacgtt gtcgatgctg agcccgcacc ggcgggtcag
     1741 gtggccgagt ccgccgccca gggtcagtcc gccgacgccg gttgtcgaga tgatgccgct
     1801 cggcgtggcc atcccgaacg cattggtggc ctgatccacc tcgccccaac gacacccgcc
     1861 gccgacggtg acggtgcggg cgtccgcgtt gacttcgatg tccctgaact tcgacaggtc
     1921 gatgacgacg ccgtcgtcgc aggtgccgag accggcaccg ttgtgcccac caccgcgaac
     1981 ggccaatggc agctcgtgtc gccgcgcgaa ggcgatggcc gtcgcgacat cggcggggtt
     2041 cgccgggcgc gcaaccagca ggggacgttt ctggatgtag gcgttgaaga ccttctgtgc
     2101 ctcgtcgtac gaggcgtcgg acctgccgat cagctcaccg ctgaagtcgg tcagctgctc
     2161 gcgggcggtg tcgacgtcga tgaccgtgct catgggctcc tcctccggga ccgtcttctc
     2221 tgtgtacggc ggtcgaccgc gttcggctat ccgggaaacc acgtacgtac cgcgtcgagg
     2281 cgtccgtcct ggctgcgtct gtccggttac gcgcccgctg tttcagcgat ctgcgggccg
     2341 ctgcgtcgct gaccaggccg ccacttcagc acggttacgg gcgtcgagct tggccatgat
     2401 gttgtggacg tgcgcctccg cggtccgggc agcgattccg agccggcgcc cgatctgctg
     2461 gttggtgcag ccgtcggcga ccaggccggc gacctcacgc tcacggacgg tgagcgtctc
     2521 ccactgcggc gtggtcggcg tagcggtcga gcgggctgca gccgcagcga cctcatatgc
     2581 gagctcgtac cccgaggcga caggcaacgt catcccgtcc tgccgtgcac ggtcgaaccc
     2641 gtagtcaccg agggcggcac gtgccgcgac agccgacgcc tcgacgcgag cgagctccgt
     2701 gccgactcgg ggcgctgcga gccggtcacg taccgcagcg gccgccgtcg ccagtcggac
     2761 cgcgtccccc ggtcgatcga cggcgaccgc cacggtggcg agcgactccc agcacagtgc
     2821 cgcaccccgc aggtctgagg cgcgctcgaa ctcccgcaag gagccggcca ggaacccggc
     2881 gccgtcgtcg atcgtgccgc cctcggccag cagtgtcccc aacgcccacg aagtccaggc
     2941 cacagcccag acgtagtcga gccgtcggaa ccggtccaac gcgtcggcca tgagtgggga
     3001 ggccgccgat ggttcaccgc gctgccaggt aacccgtccg aggtcgaagc gtgcccacgc
     3061 cgagccgagg tcgtggccga gctcgtcgta gaggcgggcc gcttcccggt gctgtgccgc
     3121 agcctccacg aagtccccgc ggtcgcgggc gacgtgcccg aggaatgcgt gcaccagtgc
     3181 catccgacgc aggttccctg tgcgccggac ggagtccaac gcccgctcga gctgtccgtg
     3241 ggccagatcc aggtcgccga cgctccacgc gaggacccca ccgatggcgc gggcgggtcc
     3301 ctcgagcgac ggatcgacgg gttcggcggc accatccacc ctggccagca cctcgtcgac
     3361 gagtgccttg cccgcgccca cgtatccatg cgtgtacgac gaccagccga gcgcggtgac
     3421 cagtggaagt gcctgctcga ggtcgttgtg cgcaaggacc ctgtcgagtg cggttcgcac
     3481 atcgcgttcg tgcggggtcg cccagtcgta ccatcgaggc tcttcgggcg tacccaggct
     3541 ggcctcgctg cgaagcgcct ggccgacgaa gtacgccgag tgtcgagcct cggcgaggct
     3601 gccgtcgtcg taccgcttcc gttgctcggt ggcgaagtcc cggacgctct gcagcatgct
     3661 gaagtgctgc tcggcgtcct cgacgtctgc gggctggacc aggctcttgt cgacgagcga
     3721 cagcatcgtg tgttcgacag cgtccggatc gaggccatcg ccgccgcaca cctgcgtggc
     3781 cacctcgagc gtccacggcg atgcaaagac cgacaaccgg cggaagagcc tgcgctcgtc
     3841 ctcggtgagg acgtcgtggc tccagctgat ggccgctcgc aggctgcgat gtcgcgccgg
     3901 tacgtcgcgc gcggtcgcct cgagcaacgc ccttcggtcc cgaagtcgcg cggcgatgtc
     3961 cgcggggctg aacaccttga cgcgcggcgc cgccagcgtc agcgccagtg ggagaccgtc
     4021 gagccgcacg cagatctcgg cgaccgcgtt ggcgttgtca ggtgagacat ggaaatcggg
     4081 gcggaccgct cgcgcagcgg ccgtgaacag cgccaccgac gggatcgcct ggaagcgtgc
     4141 aaggtccgcc acgtcatcgg ccaccggcat ggccaacggc ggaaccggga actggtgctc
     4201 ctggctcagg tgcaggttct cgcggctcgt gaccaggatc gtgaggcggg aggtcgaccg
     4261 cagcagggct gagagctcgg aaggcagtcc ggtcacctgt tcggcgttgt cgaggatcac
     4321 cagcatgtcg cgatccatca gccactggcg aacccgaacg acaccgtcct cacccggacg
     4381 gctgtcgata ccgagaccgc gggcgatctc gttcatggcg ccggactggt cgcgtgtcgc
     4441 cgacaggtcg acgaagacgg cgccgtcggc ataccggtcc gcgcagcgac ttccgagctc
     4501 ttgggcgaga cgggtcttgc ccactccggg gggaccagcc agcgtgagca gccggacgtc
     4561 tccgtcgagc aacgcggtcg tcctcgcgag ctcggcgtcc cgaccgatca ggtccccccc
     4621 aaccgggcgc acccgtttca cgtcgacacg gtagtcccga acgaccaccc tgggcccccg
     4681 gcgaggtggc aggtttcgtt ggagtttgtc ggcgtgtcga accactatcg ccacttcgat
     4741 cgcggggccg accgatcgct caggaagcaa gcacggacag atctcccgcg caggccacgg
     4801 tgtcgtggaa ccagctgagg acgttctcgt ctcggttccg ctcgtagtcg acctgccagc
     4861 cgttctgcct gaggtcggcg ttcgacgtga tgcattcgac gagcgccgct gctgtgacgg
     4921 ccgcggagtc catgccgacg ccactccacg gatcctggtg aatgccggag tcaccgacga
     4981 gcgcccaccc aggccccgca ggctggcgca cgtagtcatc gagcggaccg gaaccgaaca
     5041 acctgccgtg ccgtgtgctg tcggcgtacc gctgccacag acctgtgtgc ctcgccaaga
     5101 gttcgtcgaa cacctgcgcg gaggagtgac ggaacctcgc gtacgccgcg aggttcacac
     5161 tgatggcgac acacgtcaga ccggcatcgc tgggaaagat gtaggcgagc tcgtcatcga
     5221 gcagcgagaa ctcagggccg tccggagctc cgcccgacgg gctcggcatg tcggcgacat
     5281 atctgtagta gagcgctcgc ttcccccgat gacgctcccg gtctgctgcg ttgacagctc
     5341 gcgcaaccat cgaccgtcgt ccatcggctc cgacgacgag tcccgccatc acagtgctcc
     5401 cgtcaccgag tagcacgccg gtcacgcgtt cgccctcggt gatcaggcgg gacaccgagg
     5461 ttgcggtgcg cacgtgggca ccggctcgcc gcgcaccatc gaggagtaca gcgtccaagg
     5521 tctcgcgccg taccgacagg cagtaccccg cgtcgccagg gtcttgcgga ggctcgatcg
     5581 tcgggcttgg gtctccgttg aagtaggcgt actcgcacgt gagcctgggt ggtccgagag
     5641 caagcacggc atcgagcatt ccggccatgt ccaggacgcg tatcagcccg ttgccgcgga
     5701 agaagtgcgt cgacagcgtc gtactcggga aacgtgcccg atcgatcacc agcacccgat
     5761 ggccggcgcg cgccagcaag atggccgtga gcgagccggc gacccggccg ccgacgacga
     5821 ccacgtcgta cgtcgagccg ttcacagggg gatgtcgttg agccaaggtg tcgctgctcc
     5881 catcgcgaag agtgcgacac cgacaacgac cgcgaccgca tgcgggatgc acacgtgcgg
     5941 catccagaac gtgtggtgca gcatccgggt gatctctccg tcggtcagca ccgtcggccg
     6001 ggcgcggctc ttgtggtgtc ctgcgcgacc ggcatccgtc caggcccagt cgatccgggg
     6061 agccgaggca tcgacgccgt cgacctgcag ccggttgagc ccgaacgtgt ccacctcgat
     6121 ctcacgcatc ctcgggtagt accgcatctg atggcgcttg gtgatggcct cgaccaacca
     6181 gaacgccacc gcgctgccgg ccgccaacgc gaacaacgcg tagtggctct gctggaatcc
     6241 gaggccgatg gcggccagac tcaacgtgac tgaccagctc ttcatggtca ggagccggcc
     6301 gtcgaactcc cccacgatgc gaacgatcgc gtagtactcc gtgttcaggt cctgacggag
     6361 agcgtcggcg tcgtacgtcg cagcacgatc cttcgcgtcg ccgctgtcgt cgggcatgca
     6421 ctcatgatgc cgaggcgacg caagcgtcga ggcggtccag accccgcggg gcagcgactg
     6481 cgacggctgc cggagaacgg tcgtcgtccc aagacccacg gcccgatctc ctgcagtcgg
     6541 atcggaactc gcaacagcag cgcggctgga caacgccgct agactcccaa gattcctgag
     6601 ccaggaggcg acgtgtcccg tgcgctccat gctttggggc ggttgtgcgc acgacggtcg
     6661 ctcttggtga tcttcctgtg ggttgtcgcc ggggcagtcg tgggactggt ggtggcccgg
     6721 ttcggcaccg agaccagtaa cgacctcacg ctccccggca ccggaagtca gcaggtacag
     6781 gacctgctga gcgaccgctt cccaccgcag cagaacggca ccaacccgat cgtcttcgac
     6841 gtcgcgaagg gcaagctcac cgacgacgcg aacaagcagg cgatcaacgc ctccatcaag
     6901 gcgatgcgca aggcgccgaa cgtctacagc gtcacgaacc cgatcagcag cgacggacag
     6961 acagcgggcc tgctgtcgaa ggacgggcag acggccttcg cgccggtcct cctcgacatc
     7021 ggctccggtg agctggacga ggagatcgct cagcaggtct tcgacacgac ccacgcggcg
     7081 caggaggcag ggatcaccgt cgccgccgaa ggctcgatcg gcagcgagct gtcagaccag
     7141 ccgtccgaga cgagcgagct cgtcgggatc gtggcggcga tgctgatcct gacactcgtc
     7201 ctggggagcc tggtggcgat ggggctgccg atcatcaccg cgatcgtcgg gctgggcatc
     7261 gcgttggcct gcgtcggtct gctcggccac ctcgttgcga cacccgacac cgccgccacg
     7321 ctcgcgacca tgatcgggct cggtgtgggg atcgactacg cgctcttcct catcacacgg
     7381 caccaggacc agctcgcagc cggtgtcgag atgaaggagt cgatcgcgaa cgcagtggcc
     7441 acgtccggca gtgccatcgt gttcgccggc gggacggtcg tgatcgccct cgtctcgctg
     7501 cgcgtcgcgg gcatccccct gctcagcacg ttgggcctcg cctcggcggt ggccgtcgtg
     7561 acagcagtgc tcgccgcgat ctcgctgctt cccgcgttgc tcggactcct cggacaccgg
     7621 atccgttggc tcagcctccc ccgcttcatg cgcagcaagc accgggccgg acgtagcaga
     7681 tggacctcct gggcgggatt cgtgtcgaga cacccggtct ggatcacgct cgggtcactg
     7741 gccctgctct taccgctcat cacgccggcg acgtcgttga acctcgggca ggaagacatc
     7801 ggcgcggcat cgccggacac caccgagcgg caggcgtacg acctcatgac ggctggcttc
     7861 ggcgtcgggt acaacggccc gctgcaggtg gcgtcggagc tggacccgcc ggcgcaacag
     7921 agctcggagt acaccaagaa gtacgacaag gcgacctccc tgcagaagga cctcgagaac
     7981 aagcagaagc agcttccgaa ggagcagaag cacctcgagc aggaacagga ccagctcgag
     8041 cggcagaaga cgtcgctcca acaacagcag gcggcgctca cccgccggca gaagacactc
     8101 gagaacaagt ccgcaaggct cgaggcacaa cagtctcagc tcgaggccca acaggcgcag
     8161 ctgctcgcgc agaagcagca gctcgaacag cagcagcgag agctcgaagc gcagaagagg
     8221 cagctccagg cccagcagcg aaggctcgag gcgcaacaga cccgtctcga ggagcgtcgg
     8281 gctgagttac gggcagaacg caaagcactc gcggccgagg cccggccgtt gctgaagcag
     8341 ctggccagga tcatcgtgca tgaacggatc ctcgagcgcc gcatcgcacg gctcgacgac
     8401 aggccgcgtc tgcaggcacg gttgcaggcc cgactggacg accttcggca gcgagaacag
     8461 caggtacgcc gcgaactggc gccgttggtg cggcaggccg agcgcctcgc gcagcatgcc
     8521 cgtcggctcg cggcgcaggc cgaggagctc gacgcgcagg ctcaacggct gcaggcccgg
     8581 gccgatgacc tccagtcgca aggcgccgct ctccaacagc aggccgacca gctcgaggcg
     8641 caaggcgctg ctctccagcg aaaggccgac cagctcgaag cggaaggagc ggcccttcag
     8701 cgacaggccg accagttgca ggcacagggc gaccagctcg aacagcaggc cgccgagctg
     8761 cagcagcagg ccgaccagct ccagaagcag gcgaaccagc tgaagggcga gcagcagcag
     8821 gccgagcagg aacagcagca ggccgagcag ctccagaagc agctcaccac catgctcacg
     8881 aaggccggcg gcgaccctcg tggcaccgac gctcgtgtgg ttcggatcca ggacgacttg
     8941 gccgcgacat ccgggatcct cacgctgacg ccgccacaga tcaacaagaa gggcgatgtc
     9001 gtcctgctgt cagcggtgcc gacgacggcg ccggcgaccg tcgccactgc caacctcgtc
     9061 ggtaccgtgc gcgacgacgt cgtaccgacg tgggagaaag ccggtggcac cacgtcgtac
     9121 atcgggggtt acacggcgtc gtacgtcgac ctggcgtcgc tgatctcgca gcggcttctc
     9181 ctcgtcatcg gcaccgtcat cctgctcggc ttcctcgtgc tcatggtggc gttccggtcg
     9241 ctgctcgtgc cgctccaggc ctcgatcacg aacctgctct ccgcggcggc gtcgttcggc
     9301 gtactgacgg cgatgttcca gtggggctgg ggcacgtcgc tcgtcggact ggacaccacg
     9361 acgccggagg tgccgatcgc cagctatgtg ccgctcatga tgttcgcggt gctgttcggg
     9421 ctctcgatgg actacgaggt gttcctcgtc agccacatcc agcatcacca tcgcggtggt
     9481 gagcctgaac gcaaggcggt ggcatcgggt ctcgcctcca gcggccgcat caccaccgct
     9541 gcggcgctga tcatggcgtc ggtgttcgcc agtttcatcc tcaacggtga cccgacgatc
     9601 aagcagttcg gcgtcggact gtcggtcgcg gtgctgctcg cgggagcgct cgtcgtcacg
     9661 ctcgcgccgg cgacactgtc gctcatgggt gaggcggcgt ggtggctgcc tggatggctc
     9721 gaccgcgtcc ttccgcacat ctcggtcgag ggtgaggaac aacctcccgc tccggctccc
     9781 cggccggcgg cccaggcgac ggacacctgg cagcccgccg aatacgaggc cggagcctcc
     9841 gacatcttcc cttggcgggg tgacgacctc agcgacgacg gtggagcgac ccctcgttga
     9901 acccgactgg ttgacacgcg ctcgactccg ccgccttggc gacgttgcgc tgcatcgttg
     9961 cgaacaccag gccgtggaat ggatagacgg ctgcccagta ggcatgaccc agagcaccgt
    10021 gcgggtggaa caaggcgcgc tgccgcagtc ggctgtgggt cgcgtccacg gcggccacct
    10081 cgaactccaa ccaggcgagt ccaggcagtc gcatctcggc acggagcaac agccgcttcc
    10141 cagactcgag cgcttcgact cgccaccagt cgacggagtc tcccacgagg agccggtccc
    10201 gatcccgtcg cccgcgacgc agaccgggtc caccgaagag ccgatcgacc agacctcgga
    10261 ccgcccatcc aaggcggaag gagtaccagc cacgctcgcc tccaatgcct tcgacgacgc
    10321 tccacagctg cgtcgacgtc gccgaaacct cgatctcacg ctcgtcgacg tacaacgagc
    10381 caccggccca gtcggggtcc gacggcagcg gctcgctcgg cgcgccgggc atgctggccg
    10441 acgaccagcg cgtcggcacg tccatgtttt ggatcttcgc gagggccagc tcgacggcac
    10501 gcgggtatga cgtcaggccg gccgaagggt ccggcacgta cgccgcgatg tccttgtcgg
    10561 ccgccacgac cgtgttgcgc aaggactcca ccagtgggcg cgccagccga ccgggaaccg
    10621 gtgtcaccag cccgacccac aggctggaga gacccggtga gagcacgggc acggccagca
    10681 ttcgccgccg cggtagaccc acgacgtcgg cgtaacgctg catcatctcc aggtacgtca
    10741 gcacgtcagg tccaccgatg tcgaaccctc ggttcacatc gtccggcatc tcggcggcct
    10801 tgacgaggta gtgcaggacg tcgcggatcg cgatgggctg cactcgcgtg tgcacccacc
    10861 gaggcacgac catcaccggg agacgctccg tgagatagcg caacatctcg aatgatgccg
    10921 agccggagcc gatgacgacg ccggcgcgca gtacggcggt gggaacgccg ctggcgagca
    10981 agatgtcccc gacggccgcc cgggaccgca gatgagccga cagctcctct ccgggcgggc
    11041 tcatgccgcc gaggtagacg attcgccgca cgccccggtc ggccgccgct tccgcgaacg
    11101 tcatcgcgat ctgccgctcg gtctccccga acgacgtccg ggcgtcgatg gagtgcatga
    11161 ggtagtacgc cacagcgaca ccgtccaggg ccgcgtgcac ggcctcccgg tcaagggcgt
    11221 cggccgccac gatctccacg tcggacgccc aggggcgagc ggcggcccgg ccggggtcgc
    11281 gtgccatcac gcggacctgg tgtccgtggt ccaacagctc gggcaccagc cgtccgccca
    11341 cgtaccccgt cgcgccggtg accagggtca gcctcgtcgc gtcgccggtc atcgggccgc
    11401 ctggtcgact tcggcgtgcc ttcccgggcc ggtgcaggca cggctcgtca gggccgacca
    11461 gacagcgacc ggcagcactc ggggcgcaac cgtccaggtc atggatgcgt cacgtccttg
    11521 gcttgtctag cgtatgtcta gattcaggac accatgcctc cgcgcggctg tcaaggattt
    11581 gtccatagtt tgctagggtt ctgtccatgg cacccgctcc ggccgtggcg atcgcgctgt
    11641 tcacgcgaaa cttgcgcgtg cacgacaatc ccgtgctcgt cgccgcttcc cgacatgagc
    11701 acgtcgtccc gttgttcgtg ctcgacgacg gttgccgtcc gttcgtgtcg gcgaaccggg
    11761 cagcctttct cgtcgactcc ctcgaagacc tgagcagtgc gctccgggcg cgaggcggtg
    11821 cactcgtcgt acgccggggg aaggttgtcc acgaggtgtg cgcactcgcc cggcagaccg
    11881 gcgcgaccca ggtgcatgtg gcggccgacg tgagccggta cgcccagcgc cgagagcatg
    11941 cactggcagc cgcgctgcga gcggagggtt gtgagctggt cgtgcacgaa ggcgtggtga
    12001 cagtggttcc accaggcgcc gtcactcctc agacgaagga tcactatgcg gtgttcacgc
    12061 cctacttccg gcgctggctc caacaaccgc gccggcccct cctcgatcca ccgcgacggt
    12121 tcacgctccc caggctccat gccggagcga tccctcgcgc cgcggacctg tgccaaggag
    12181 caacgtcgga ccggctgatg cgaggaggtg agcgcgccgg acgggcacgg ctcaccagtt
    12241 ggagcaggaa ggtcgatgcc tacagcgagc gtcgtgacgc gttgagcggc gacggcacgt
    12301 cgcggttgtc ggcgtacctc cacttcggct gcgtttcgcc ggtggaggtg atccaccgca
    12361 tcggacatga cagtgccggc cgtcgcgact tcgttcgcca gctcgcctgg cgggactttc
    12421 accaccagct gctggccgcc cgacccgaat gctcgtgggc ggactatcgg ccacgcgggg
    12481 atcagtggcg tgacgaccat gcggcgttcg aggcctggcg ggcgggacgc accggaatcc
    12541 ccgtcatcga cgcggcgatg cgacagctgc ggaccgaagg ctggctgcac aaccgtgccc
    12601 gactcctggt cggcagcttc ctcaccaaga cgttgtacat cgactggcgg tggggcgccc
    12661 ggcacttcct cgactggctc gtcgacgcag acatcgcgaa caaccagctg aactggcaat
    12721 gggtggcagg tacaggggcc gacacgcggc cgaaccgagt gctgaaccct gtcgtccagg
    12781 ccctccggca tgatcccgac gggaggttcg tccggcggca cctgtcggag ctggccggca
    12841 tcgccgggcc tgccgtacac caaccgtgga agcttccgcc ggacgtacgc cggtcgctgg
    12901 actatcccga cccgatcgtg gacatcgaca cggcgggacg cggtttcagg gcgggccgtg
    12961 ccgggagatg atcgtccggc gggcggttct gggccggctc cgtcatgatc ggttggcctt
    13021 ggcaggtagt gacttcgcgt atgccacacc acgggcaacc cattcctcga gctgacccgg
    13081 tgacatcaca gccgcggtct cgacccgcag ccatccactc atctgtcggc cgcgcatctc
    13141 catcggctcg acgtgcgcac cgtcgaccag cgcctccgac tcctcgggat ccacccggac
    13201 caacaagccg ccttgcccgc tggcagcgac cgacatgttt ccgttgatca ggaaggccaa
    13261 gccgccgaac atgcgctgtt cggagacacc ctgctcgccg gcgaccagac cgcgaatgcg
    13321 atccgccagc tcctcgtcgt acgccattcc ttgattgtcc acggggcacc gacagtgcga
    13381 cgcgagacgt ctggcgccgg agtacccacc agggatatgc cgagcgtccc cctcagggcg
    13441 atcgctcgcc caggcatgat cgaaggatga acaccccggc ccggtacgac ggcagcgcgc
    13501 agtggtacga cgagcgtttt cgccactacg gcgacctcac tgaagtggac tcgagcgcga
    13561 gcttgcttgc caccgtcctc ggccgaggcg acgggtggtg tctcgacgtc ggctgcggga
    13621 ccggattgca tgcggccgcg atcgagtcga ccggcaggcg ggtgttcggt gtcgacctgt
    13681 cggccgacca gctggcccac gccgcgcacc ggatcgccgt ccgggtacgg accgacgcga
    13741 ccaggctgcc cttccgcgac ggtaccttcc cctcggcggt gtgcacgtac gtccacaccg
    13801 acatcgagca gatcgggcaa gtgttcgccg aggtatgccg ggtgatgcgc cacggcggaa
    13861 ccttcgtgta cgtcggcgtc catccctgct tctggggccc gcacatcgag cgggtcgacg
    13921 agacgacacg ggttgtccac ccgggctact gggaatgctc atggcggacc cgatcgccgt
    13981 actggagcga cgtggggctg gcctcccggg tcggcttccg gcatcggacg ttggccgacc
    14041 tgttgacgga gctgttgtcc agcggcctgc ggctcactca cgtcgctgaa ggggcgcgtg
    14101 gggcaccgtt cgccgaccgg atcgccatcg cggccgtgaa ggagtgaggc ggacggtcca
    14161 tgcgcggttg gctgcgtgtc gacgcggcgg ccgctcgaac gtcagatcgc ctcgaacgat
    14221 gggtgacgcg cggccgtcgc atacgcgaaa gtcgctgccc gcgaagtcca agactcgctg
    14281 atgttcccgc gcgaccggcc agttctccta tcggtcggtt ctccttcggg gtttgcgatg
    14341 tttacagccc gggcgccgtg tcaacatcga ggatctgcgg tccacggtag gggtccgcga
    14401 tgtgaacatc gcggattgcg ggagatgagc ggcggtttgc acggctaccc aggcagaccc
    14461 acccctcgca aggcagatct gcctgg
//
LOCUS       DATBFZ010000447         6244 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_973988, whole genome shotgun sequence.
ACCESSION   DATBFZ010000447 DATBFZ010000000
VERSION     DATBFZ010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 6244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6244
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_973988"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            270..1223
                     /locus_tag="VH419_13605"
     CDS             270..1223
                     /locus_tag="VH419_13605"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEY7044699.1"
                     /translation="MGAGTASTGAYEPADTRRSRLRRLLPVARVVVPLLALVVLLQRF
                     GAEAFRPALEVVSPIPLLAALVLGGVAVAAQSARWRIVMRGSGLPLGRREALVECYRS
                     CALNTVLPGGVAGDVLRAWRQRTGAPKGWQPGAVSVAAERVAGFSVLLVAVSVVLFID
                     GEPMLAAAAATLASIAWLVTRRPLGRLGWRDRAAVWGLSLVALVALVGLMVVVAAAIG
                     VSERPNVIATFGLVLLAGMSIPLNIGGWGPRETAGALVAPLVGAHPATGIAVAAGYGV
                     LAAVSVLPGFLALALPRLTGDRQQRAASSDTDSESRTVAKV"
     gene            complement(1163..1903)
                     /locus_tag="VH419_13610"
     CDS             complement(1163..1903)
                     /locus_tag="VH419_13610"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEY7044700.1"
                     /translation="MSAEREVVRDATMAIVGGLVVAAVVATFAGRFQAASTSTMAIGV
                     VLGGVPAALAGASVVCRRPQVATPADRVTLVRAVLTSGCAAAAAMAPISDLDVSSWWF
                     VSLAALALLLDALDGAVARSSGTTAAGARLDMELDAGMLVVLSVAAATQLGPWVLLVG
                     AMRYLYLVASYLWPLLGTSVPSSRFRRTVAGLQGAALTVALAPFTPVDVGTAVVIASL
                     VLLVASFAHQTLATVRLSESVSDEAARC"
     gene            1935..2924
                     /locus_tag="VH419_13615"
     CDS             1935..2924
                     /locus_tag="VH419_13615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017935921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HEY7044701.1"
                     /translation="MRDDRRVTVTATAYWTLGPGQGTLRDEPLRDPGPDEAMVRTRHS
                     GISKGTELLVHNGSVPAAVAELMRAPFQEGDFPGPVKYGYQSVGVVEQGPDALLDREV
                     FCLFPHQDRYVVPVTALTPLPDGVPARRATLTGAVETAVNALWDAAPRLGDRVAVVGA
                     GMIGCAVAGLLRKLPLGRLELVDVDPARASVASRLGVGLVSPDAAAGGCDLVVHASAS
                     SAGLRRSLELLGDEGEVIELSWYGTEEVSVPLGSVFHPRRLAIRASQVGQVATARRSR
                     RTHAERRALALEMLRDDAFDALITGSSAFGDLPDTMRRLASGELPALCHVVDY"
     gene            2955..3332
                     /locus_tag="VH419_13620"
     CDS             2955..3332
                     /locus_tag="VH419_13620"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013861027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HEY7044702.1"
                     /translation="MYAVTVRDHVMVAHSLPRPVFGPAQGLHGATYVVEATFRRRDLD
                     EDGIVVDIGAATAALDEVLDRLRYRNLDEHPDFHGVLTTTEVLTRWVADRLDEAPVSR
                     QVDGLEVTLRESPEAWVSYTREP"
     gene            3329..4318
                     /locus_tag="VH419_13625"
     CDS             3329..4318
                     /locus_tag="VH419_13625"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY7044703.1"
                     /translation="MRLRLVVPANVDSPTGGNVYDVSMADALRRQGHGVEVVRCQPSG
                     LSDVLRRPWSGPTLVDGLLACRDPQALASRDIGVLVHMPLAWETGLSSEHAAELDGQE
                     EQALLAARVVVCTSHWCAHHLAHRHGVQHVVVAPPGVDPAPLSTGSDPPLLVQVAALL
                     PHKDQLTVVAALRRLTGLSWRARLVGSVDRDPEYAATVRDAVSSAGLTDRVDMPGEVS
                     RDDAYAGADLALLPSRAEAFGLVVTEALARGVPVVVSETGAAEALGLTHTGRRPGMVV
                     PAGDVDALSDALRRWLTDASFRNDLRSRALERRETLEGWEATAKRIVSALTAT"
     gene            complement(4333..5760)
                     /gene="ribA"
                     /locus_tag="VH419_13630"
     CDS             complement(4333..5760)
                     /gene="ribA"
                     /locus_tag="VH419_13630"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF001591.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HEY7044704.1"
                     /translation="MSAELTAWPRVTRVATTRIPTRHAEFVAYGYRDEHTGEDHVVLV
                     LGDLADADGPPPLVRLHSECLTGDALGSRRCDCRDQLDSAQAAIAAEGRGAVVYMRGH
                     EGRGIGLSAKLRAYELQDDGLDTLDANVALGLPVDAREYGAAVAVLNDLGVSSLRLLS
                     SNPAKEEGLRAQGMVVERLALPVPRRPENVRYLRTKQERMGHDAVAYDDAEQPWRELL
                     AGRVPSRGELADRYGWMVQAGDRFVVAQLGQSIDGFIAARTGDACYVTGDADRTHLHR
                     MRALVDAVVVGVGTVLADDCRLSVRSVSGPNPVRVILDPRGRVPRDAQLLTNPEAPTL
                     WLVADDRPPLSTGASHVDVVALPRSGPARGFAPADVLGLLGDRGLGRVLVEGGGVTVS
                     RFLAAGALDRLLVTTAPLLVGDGVPGLRFNGQDRLADALRAPSRHFVLGGDVCLELDL
                     AAARRNGNGSEVRDGHPVHTLPTSS"
BASE COUNT          882 a         2184 c         2204 g          974 t
ORIGIN      
        1 cggactcctg ctcggcacga agggttggcc cagtccctag cgcatggcga tgacctggtt
       61 gccgggtcag tcgatcgtcg actggcgcac tcacgggtgt ccgcgcccga ggaagacgac
      121 gccggggtcg tccgccgagg cccgtcgagg tggcaatgac ggttcgacac gccgacgaac
      181 tccaacgaaa gttgccacct cggcggggtt gggggcggtt gcccgataag gcaatggtgt
      241 ggacgcaagt ggaccgggag actcgatgta tgggagctgg caccgcgtcg accggtgcgt
      301 acgagccggc ggacacgagg aggtcgcgtc ttcggcggct gctgccggtc gcccgggttg
      361 ttgtgccgct ccttgcgctg gtcgtgctgt tgcagcggtt cggcgcggag gctttccgtc
      421 cggcgctcga ggtcgtgtcg ccgatcccgt tgctggccgc gttggtcctc ggtggcgtcg
      481 ccgtcgcagc gcagtccgcg cgctggcgga tcgtcatgcg tgggtccggg cttcccttgg
      541 ggcgccgcga agcactggtc gagtgctatc gatcgtgcgc cctgaacacc gtcctgcccg
      601 gcggggtcgc cggggatgtc ctgcgcgcct ggcgacaacg caccggcgcg cccaaaggat
      661 ggcagccggg ggcggtgtcc gtcgccgcgg agcgggtcgc cgggttctcg gtgctgctcg
      721 tggcggtgtc cgtggtcctc ttcatcgacg gagagccgat gctcgcggcc gctgcggcaa
      781 cgctcgcgtc catcgcctgg ctcgtcaccc gacgtccatt ggggcggttg ggctggcgcg
      841 acagggcggc ggtgtggggg ttgtcgctgg tcgcactcgt ggccctggtg ggcctgatgg
      901 ttgtcgtagc cgctgcaatc ggcgtgtcgg agcgacccaa cgtgatcgcc acgttcggcc
      961 tggtcctgct cgcgggcatg tcgatcccgc tcaacatcgg cggctgggga ccacgcgaga
     1021 cggccggtgc cctcgtcgct ccgcttgtcg gggcacaccc cgcgaccggg atcgcggtgg
     1081 cggccgggta cggcgtactt gcggccgtct cggtgctccc agggttcctc gcgctcgccc
     1141 taccccgact gaccggagat cgtcagcagc gggcggcctc gtctgacacg gactccgaga
     1201 gtcgcaccgt cgccaaggtc tgatgcgcga aggacgccac gagcaggacc agagaagcga
     1261 tcacgacggc cgtccccaca tcgacaggag tgaacggcgc cagcgccacc gtgagcgccg
     1321 ccccctgcag accggccacc gttctgcgga accggctcga cggaacggac gtgcccagca
     1381 gcggccagag gtatgacgcc accaggtaca ggtagcgcat cgcgccgacg agcaggaccc
     1441 aaggacccag ctgcgtcgcc gcggccacgc tgaggacaac gagcatcccc gcatcgagct
     1501 ccatgtcgag acgagcaccg gcggcggtcg tcccggagga acgcgcgacc gctccgtcga
     1561 gcgcatcgag cagcaacgcg agcgccgcca gcgacacgaa ccaccagctc gacacgtcga
     1621 ggtcgctgat cggagccata gccgccgcgg ccgcacaccc actcgtcagg actgcgcgga
     1681 cgagggtcac gcggtcggca ggggtcgcca cctgtggacg tcgacacacc accgacgccc
     1741 cggcgagcgc ggccggcacc ccacccagca cgacacctat ggccatcgtc gacgtcgaag
     1801 cggcctggaa cctgcccgcg aacgtggcga cgaccgcggc gacgacaagg ccgccgacga
     1861 tggccatcgt cgcgtcgcgg acgacctcgc gctcagcact catcaggtgc tccgtcgtgt
     1921 acggtcccaa catcatgcgc gacgatcggc gcgtcaccgt cacggcaacc gcctactgga
     1981 cactcggccc gggacagggc accttgcgcg acgagccgct gcgcgatccc ggcccggacg
     2041 aagcgatggt ccggacccgc cactcgggga tcagcaaggg gacggagctc ctggtgcaca
     2101 acggctcggt cccggcggct gtcgcggagc tcatgcgtgc cccgttccag gagggcgact
     2161 tcccaggccc cgtcaagtac ggctaccagt cggtcggggt cgtggagcag ggccccgacg
     2221 ctctgctgga tcgggaggtg ttctgcctct tcccccatca ggatcggtac gtcgtgccgg
     2281 tgactgccct gacgccactt ccggacggcg taccggctcg acgagcgacg ctcaccggtg
     2341 cggtcgagac agccgtcaac gcgctctggg atgcggcccc tcggctcggc gaccgggtcg
     2401 ctgtcgtggg cgccggcatg atcggatgcg cggtcgcagg actgttgcgg aagcttccgc
     2461 tcggccgact ggagctcgtc gacgtcgacc cggcgcgagc ctcggtcgcg tcgcggttgg
     2521 gggtcgggct ggtctcgccc gatgcggccg cgggcggctg cgatctcgtc gtccacgcat
     2581 cggcgtcctc ggcggggctc cgccgcagcc tggagctcct cggcgacgag ggcgaggtca
     2641 tcgagttgtc gtggtacggc acggaggagg tcagcgtgcc gctcggttcc gtcttccatc
     2701 cacggcggct cgcgatccgg gcgtcgcagg tgggccaggt cgcgaccgcc cgacgcagcc
     2761 ggcgtaccca cgccgaacgg cgggcgttgg cactggagat gctgcgcgac gacgccttcg
     2821 acgcactgat caccgggtcc agtgcgttcg gcgatcttcc cgacacgatg cgacggctgg
     2881 cctccggcga gctcccggcg ctctgccatg tcgtcgacta ctgagtccac cgaacgggac
     2941 agtgaggaga ggcgatgtat gccgtgacgg ttcgcgacca cgtgatggtg gcgcacagtc
     3001 ttccgcggcc tgtcttcgga cccgcgcagg ggttgcatgg agccacctac gtggtcgagg
     3061 cgaccttccg tcggcgcgac ctcgacgagg acggcatcgt cgtggacatc ggtgcggcca
     3121 ccgctgccct ggacgaggtc cttgaccggc tgcgctaccg caacctcgac gagcacccgg
     3181 acttccacgg cgtactcacc accaccgagg tgctcaccag gtgggtggcc gaccgactcg
     3241 acgaggctcc ggtctcccgg caggtcgacg ggctcgaggt cacgttgcgc gagtcccccg
     3301 aggcgtgggt ctcctacacg agggaaccgt gaggctccgt ctcgtcgtac cggcaaacgt
     3361 ggactcgccc accggcggaa acgtctacga cgtctcgatg gcggacgcgc tgcgtcgcca
     3421 gggacacggc gtcgaggtgg tccgttgtca gccgtccggt ctgtcggacg tgctgcggcg
     3481 tccgtggtcg ggtccgacgc tggtggacgg gctcttggca tgtcgtgacc cgcaggcgct
     3541 ggcgtcgcgg gacatcggcg tcctggtcca catgccgctt gcctgggaga cgggactgtc
     3601 atcggaacac gcagccgagc tcgacgggca ggaggagcag gcgctgctgg ccgcccgcgt
     3661 cgtcgtctgc acgagccact ggtgcgcaca tcatctggcg caccggcacg gtgtgcagca
     3721 tgtcgtcgtc gcgccacccg gtgtcgaccc ggctccgctg tcgaccggct ccgacccgcc
     3781 gctcctcgtc caggtcgccg cgctgctccc gcacaaggac cagctcactg tcgtcgctgc
     3841 cctcagacgt ctcaccggcc tgtcttggcg cgcccggttg gtcggttcgg tcgatcgtga
     3901 cccggagtac gccgcgacag tccgcgatgc cgtgtcctcc gccggtctga ccgaccgggt
     3961 cgacatgccc ggagaggtgt cgcgcgacga cgcctatgcg ggtgccgatc ttgcgttgct
     4021 gccgtcgcgg gcggaagcct tcggcctggt cgtgaccgag gccctcgccc gtggcgtccc
     4081 ggtcgtggtg agcgagacgg gtgccgccga ggcgctcggt ctgacccata ccggccggcg
     4141 accgggcatg gtcgtcccgg caggtgacgt cgacgcgttg agcgatgcgc tgcgtcgatg
     4201 gctgaccgac gcgtccttca ggaacgacct gcggagccgc gcgctcgagc ggcgggagac
     4261 cctcgaaggc tgggaggcga cagcgaagcg catcgtctcg gccttgactg ccacgtgagt
     4321 cggcggccgc gctcatgaac tggtgggcag tgtgtgtact gggtgaccgt cccgtacctc
     4381 gctgccgttg ccgttgcgac gggcggcagc gaggtcgagc tcgagacaga cgtccccgcc
     4441 caggacgaag tgacgcgacg gtgcccgcag ggcgtcggcc agccggtcct gcccgttgaa
     4501 acgcaggccg ggtacgccgt cgccgacgag cagcggggcg gtcgtcacga gcagccggtc
     4561 gagcgcgccc gcggcgagga accgcgacac ggtgacgcca ccgccttcga ccagaacgcg
     4621 tccgaggccg cgatcaccaa gcaggcccag cacatcggct ggtgcgaacc cgcgggccgg
     4681 gccggaccgc ggaagcgcca ccacatcgac gtgcgatgcg ccagtcgaca acggtggtcg
     4741 gtcgtcggcc accagccaca gcgtcggcgc ctcaggattc gtcagcagct gtgcgtcccg
     4801 cggcacccgc ccacgcgggt cgaggatcac ccggacgggg ttgggccccg acaccgatcg
     4861 gaccgagagg cgacagtcgt cggcgagcac ggtcccgacg ccgacgacga ccgcgtcgac
     4921 caaggcccgc atccgatgca ggtgggtccg gtcggcgtcc ccggtgacgt agcacgcgtc
     4981 gcccgtccgc gccgcgatga aaccgtcgat gctctgtccc agctgcgcca cgacgaagcg
     5041 gtccccggcc tgcaccatcc acccatagcg atcggccagc tcgccccgac tcggaacccg
     5101 cccggccagc agctcccgcc atggctgctc cgcatcgtcg tacgccacgg cgtcgtgacc
     5161 catccgctcc tgcttggtcc gcaggtagcg cacgttctct ggtcggcggg gcaccggcag
     5221 agccagccgc tcgacgacca tgccttgtgc gcgtagcccc tcctcctttg ccgggttgct
     5281 cgacagcagt cgcagggacg agacgccgag gtcgttcagc acggccacgg ccgcgccgta
     5341 ctcccgggcg tcgaccggca ggccgagtgc gacgttggcg tcgagcgtgt cgagcccgtc
     5401 gtcctgcagc tcgtaggcgc gcagcttggc cgagagcccg atgccgcgtc cctcatgacc
     5461 acgcatgtag acgacggcgc cgcggccttc tgccgcgatg gccgcctgcg ccgagtcgag
     5521 ctggtcacgg cagtcgcagc gtcgtgagcc gagggcgtcc ccggtgaggc attcggagtg
     5581 cagcctgacc agtggcggcg gcccgtcggc gtcggcgaga tcgccgagca cgagcacgac
     5641 gtgatcctct ccggtgtgtt cgtcgcgata gccatacgcc acgaactcgg catgccgggt
     5701 cgggatccgg gtcgtcgcga cgcgcgtcac ccgcggccac gcggtgagtt cggcactcac
     5761 acagacacct ccgctaccag gtgtgatcgg ttgatgccac tgtgcccggc tcacgtggct
     5821 tccgccatcc gacctccggg cacccgccac gcgcgtcccg gccggccgtt ggaaggtggt
     5881 tggcgggtga cggacatccg ccggtccacg gcccgctgtt tgcggcgtcc gcgggcagac
     5941 ccatcctctc aaggcccgat ccgagaactt cggagttctc attggcggag atggtctcga
     6001 tgggtgggct cgtcgcccgg cgtccgtcgt catcggttga cgtgaaatgg catcgaagcg
     6061 gggaacgttc cggaccgctg agttggtcgg ccgacttccc gtccctttgc ctggcgtctg
     6121 actggcggtt ttcgtacatc gcggctgccc ggcctgatgt ttgcgatgtc cacatccgaa
     6181 accccctctg tccatcggag gtttgcgatg tttacacgcc ttttgggctg taaacatcgc
     6241 aaac
//
LOCUS       DATBFZ010000448         2330 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_1301376, whole genome shotgun sequence.
ACCESSION   DATBFZ010000448 DATBFZ010000000
VERSION     DATBFZ010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1301376"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..771)
                     /gene="ligD"
                     /locus_tag="VH419_13635"
     CDS             complement(<1..771)
                     /gene="ligD"
                     /locus_tag="VH419_13635"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012922924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEY7044705.1"
                     /translation="MPEKVTVEIDGHKLGLSNLEKVLYPRAGFTKGEVIDYYVNVGDA
                     LLPHLSDRLLTRKRWPDGTAAQPFFEKNTPRGTPDWVRTVFMAEHGDDGVEYVVAGDL
                     ATLVWLANLAALELHVPQWKVGPRGGPQDPDLVVFDLDPGEPADIIDCCNVAFALKEL
                     LEADGLTAFPKTSGNKGAQLYVPIEPTTSRRTSDYAKRLADQLAEALPGEVVSSMEKR
                     IRKGKVFIDWSQNNAAKTTIAPYSMRGIDRPTVSTPLTW"
     gene            1052..2242
                     /gene="moeZ"
                     /locus_tag="VH419_13640"
     CDS             1052..2242
                     /gene="moeZ"
                     /locus_tag="VH419_13640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/sulfurtransferase MoeZ"
                     /protein_id="HEY7044706.1"
                     /translation="MSFPPLIEPADELTIDEVRRYSRHLIIPDVGMAGQKRLKNAKVL
                     VIGAGGLGSPALLYLAAAGVGTLGIAEFDEVDESNLQRQVIHGQRDIGKPKAVSAQES
                     VAEVNPYVNVVVHNERLDNDIVMGIFEQYDLIVDGTDNFATRYMVNDAAVLLGKPYVW
                     GSIYRFDGQAAVFWAEHGPCYRCLYPEPPPPGMVPSCAEGGVLGVLCASIGSIQVNEA
                     IKLLTGIGEPLIGKLMIYDALEMEYRKLAVRKDPNCAICGDNPTVTELIDYDNFCGAI
                     SDEAAEAAADSTISVVTLEHWLKERENGERDFVLVDVREPNEYEINKIPGSVLIPKGE
                     FLNGNALGDMPSDKQVVLHCKTGVRSAEALAVLKGAGFSDSVHVGGGVSAWVSQIDPS
                     QPSY"
BASE COUNT          399 a          765 c          760 g          406 t
ORIGIN      
        1 ccaggtcagc ggcgtggaca ccgtgggtcg gtcgatgcca cgcatcgagt acggcgcgat
       61 cgtcgtcttg gccgcgttgt tctggctcca gtcgatgaag accttgccct tgcggatccg
      121 cttctccatc gaggacacga cctcgcccgg caacgcctcc gcgagctggt cggcaagccg
      181 tttggcgtag tcggacgtac gccgcgaggt ggtcggttcg atcggcacgt agagctgcgc
      241 gcccttgttg ccggacgtct tcgggaaggc cgtcaatccg tcggcctcga gcagctcctt
      301 gagggcgaac gcgacgttgc agcagtcgat gatgtcggcg ggctcgcccg gatccaggtc
      361 gaacacgacc aggtccgggt cctgcgggcc accccgcggg ccgaccttcc actgcgggac
      421 gtgcagctcg agcgccgcca ggttggcgag ccagaccagc gtcgccaggt cgccggcgac
      481 gacgtactcg actccgtcgt cgccgtgctc ggccatgaag acggttcgca cccagtccgg
      541 cgtgccgcgc ggcgtgttct tctcgaagaa cggctgtgcc gcggtgccgt cgggccagcg
      601 cttgcgggtg agcagccgat cggagaggtg cgggaggagc gcgtccccga cgttgacgta
      661 gtagtcgatc acctcgccct tggtgaatcc ggccctcggg tacagcacct tctccaggtt
      721 ggacagcccc agcttgtgcc cgtcgatctc caccgtcacc ttctccggca tagctcctac
      781 cttcccaccc gcctccgttg cccgcccctg gtgggtttgc gatgtttaca gcccaggcgg
      841 gctgtgaaca tcgcaaacct gacgtgctga ctggtggttt gcgatgcagg cctctggtcg
      901 gccagtcagg tcgtatgccg ggacgcgtcc accgtcgcct gcagtcgtca gggaacatgg
      961 agagacgtgg tgtggttgta ccgacaggcg atcaccccag tctgtgagct gcgcccgctg
     1021 aaggacgtcg aacgtcgtac gaggagaaac cgtgtcgttc ccaccgttga tcgagcctgc
     1081 tgacgagttg acgatcgacg aagtccgccg ctacagccgg cacctgatca tccccgacgt
     1141 cggtatggcc gggcagaagc gcctcaagaa cgccaaggtg ctggtgatcg gggccggcgg
     1201 tctcggcagc ccggcactgc tgtacctcgc ggcggccggc gtcggaacgc tcggcatcgc
     1261 cgagttcgac gaggtggacg agtcgaacct gcagcgccag gtcatccacg ggcagcgcga
     1321 catcgggaag ccgaaggccg tttcggcgca ggagtccgtc gccgaggtca acccgtacgt
     1381 caacgtcgtc gtccacaacg agcggctcga caacgacatc gtgatgggga tcttcgagca
     1441 gtacgacctg attgtcgacg gcaccgacaa cttcgccacc cgctacatgg tcaacgacgc
     1501 ggccgtgctg ctcggcaagc cgtacgtgtg ggggtccatc taccggttcg acggccaggc
     1561 ggccgtcttc tgggccgagc acggtccctg ctaccgctgc ctgtacccag agccgccgcc
     1621 ccccggcatg gtcccgtcgt gcgccgaagg cggcgtcctc ggcgtactct gcgcgagcat
     1681 cggctcgatc caggtcaacg aggccatcaa gctgctcacc gggatcggcg agccgctcat
     1741 cggcaagctg atgatctacg acgcgttgga gatggagtac cgcaagctgg ccgtgcgcaa
     1801 ggatcccaac tgcgccatct gtggggacaa ccccaccgtc accgagctga tcgactacga
     1861 caacttctgt ggtgcaatca gcgatgaagc cgcagaggcg gccgccgact ccaccatctc
     1921 tgtggtgacg ctggagcact ggctcaagga gcgtgagaac ggcgaacgcg acttcgtcct
     1981 ggtcgacgta cgcgagccga acgagtacga gatcaacaag atcccgggct cggtgctgat
     2041 cccgaagggc gagttcctca acgggaatgc actcggtgac atgcccagtg acaagcaggt
     2101 cgtgctccac tgcaagaccg gggtccgttc tgctgaagcg ctggcggtgc tgaagggcgc
     2161 cggcttctcc gactcggttc acgtgggtgg cggtgtctcg gcctgggtga gccagatcga
     2221 cccctcacag ccgtcgtact gagttggtcg tacgccgctc aaggaaccac aactcacggt
     2281 tcgaggaacc acaactcacg gttcgacgaa ccacaactca cggttcgacg
//
LOCUS       DATBFZ010000449        14440 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_974420, whole genome shotgun sequence.
ACCESSION   DATBFZ010000449 DATBFZ010000000
VERSION     DATBFZ010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 14440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14440
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_974420"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..531)
                     /locus_tag="VH419_13645"
     CDS             complement(1..531)
                     /locus_tag="VH419_13645"
                     /inference="COORDINATES: protein motif:HMM:NF042550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6636 domain-containing protein"
                     /protein_id="HEY7044707.1"
                     /translation="MARTDAVHQTNTERPSTPQAPTTSDPSTPLESTPPSTTSGPTGS
                     SQSPRPPQTARRHLPNIIGGVPVFLTPSRNIGCAIGAGQARCDIAKHVYRDPPKPAGC
                     HGDYGQSIAVTAHGIASFLCVTDTVRNPGAPVLDYGTSTVVGDFGCTSRQSGIRCYHL
                     ESDHGFWLSQEHPALF"
     gene            complement(749..1684)
                     /locus_tag="VH419_13650"
     CDS             complement(749..1684)
                     /locus_tag="VH419_13650"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY7044708.1"
                     /translation="MNAQPDTFVRFVDTLAGCLDDHEVRGDDLAARAYLSRFHFDRVV
                     RATAGETPARFRRRVLLERSAYRLLTSGRGVLDIAVEAGYSSNEAFTRAFQRAYGVGP
                     ARWRSKPHRRIQLDSPNGVHFHPPGGLRLPARIEVSSMDLLVKMVEHHVWLVGELVDR
                     ANRLDDAASPGDGLDRPIQVSVDEGDDVTSLRHLLSRLIGQMDMWNCAIANEAYDWDV
                     EQHEPAAAMRTRLDRVGPVYLGHIREVCEQGRLDETFVDAVCDPPEVFTYGGMIAHVL
                     TFAAYRRTLAVQALDALGISDLGWGDPMRWVAEPA"
     gene            complement(1743..3365)
                     /gene="menD"
                     /locus_tag="VH419_13655"
     CDS             complement(1743..3365)
                     /gene="menD"
                     /locus_tag="VH419_13655"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017933147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HEY7044709.1"
                     /translation="MSASGDLAVDVVGTLRRGGVHDVVLAPGSRSAALAIAFHEADRR
                     GLLRLHVRIDERTAGFLALGVAKGSHRPVAVATTSGSAVANLHPAVLEAWHAGEPLIV
                     LSADRPALLRGTGANQTTDQVGIFGSVVPCTDVAVDARTQAVEAIAEAVRRSGPSHVN
                     LQFAEPLLPDPAQVSAALAAEPPSRRQARRRWSRDADPDSELLSPGLRTVVVAGDDAG
                     PPARLLAETANWPLLAEPTSGARTGTHAMRTSRLLLAGSLADDVERVVVTGHPTLSRP
                     MTRLISRTDIEVISVRGRGGVCTDPGRVARHVDAVPAVEKPDDEAWMDRWRSADAALS
                     ARVDSIAARDEGLPLRVAGAVAESVPPAGLLVVGSSQPVRDLDIMTTPYPSGERRLIV
                     GNRGLSGIDGTVSTAVGAALGRRSARSLAYVGDLTFLHDANGLVLGPDEPRPDLTIVV
                     ANDDGGAIFTTLEQGAPEYADAFERVFGTPHAVDLASLCASTRTAYERIETIDRLQAA
                     LVEPATGVRVLEVPLSRDRRRALGEELKALVS"
     gene            complement(3362..5395)
                     /locus_tag="VH419_13660"
     CDS             complement(3362..5395)
                     /locus_tag="VH419_13660"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: protein motif:HMM:NF002782.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HEY7044710.1"
                     /translation="MTDEASLRPLSGDARALERLLGRWLGDEAGSPLTVRTSGSAGEP
                     KEVLLSASAVRASALATLTRIGGPGQWLLALPPQYVAGLQVLVRSLLAATSPVVLEEY
                     PDVATATAAVTGERRYAALVPTQLHRLLVSDANSLAAYDVVLLGGAAAPDALLDRARE
                     AGVNVVTTYGMSETCGGCVYDGVPLDGVAVALGSAGEIRLSGPVLFDGYAERPDLTET
                     VLRDGWFHTSDIGGLDHDGRLVVLGRSDGVVITGGVNVPLSVVESRLQEMPGLTGVAI
                     TSRPDPEWGVEVVAVAARAPALPTLDAIRDFVAEVHPREWAPRSLVVVDELPMLSSGK
                     VDTRRLPTLGSSDPLRSRPPVLRGTTREVAVFSLPMNVRFRGLRRREGMLIKGAAGVG
                     EFSPFWDYDATESAAWLAAALEAADQPFPTPLRDLVPVNCTVPAVTPDEAKEIVARSH
                     GCRTAKVKVAEPGQTLDDDVARVAAVREALGPGGRIRVDANGGWPVDNAVKAIAGLAE
                     FDLEYVEQPVATVEDLALVRRRVDVAIAADESIRRASDPLRVKQLAAADIAVLKVQPL
                     GGVRACLRLAEQISMPVVVSSALESSVGIAAGVALAAALPELPYACGLATTAMFTADV
                     VDDPLTPVEGMLDVRRIAADPAALDAVRADDATAGRWHHRLAACAAVLEADRR"
     gene            5456..6340
                     /locus_tag="VH419_13665"
     CDS             5456..6340
                     /locus_tag="VH419_13665"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020579527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HEY7044711.1"
                     /translation="MTTATTTAAHWIEGARPRTLPAAVSPVLAGTGSAVYADSFVWWK
                     AVLALVVALALQVGVNFANDYSDGIRGTDTNRVGPLRLVGSGLVAASRVRAAAFLAFA
                     VAGVAGLVLAATTTWWLLLVGAVAIVAAWFYTGGSRPYGYHALGEVSVFVFFGLVAVV
                     GTAYVQTEDVTLDAVLAGCGVGALACAILVANNLRDIPTDREAGKQTLAVLLGDHRAR
                     VLYVALLAAALLLAVALAVSTSAWLLLAFVAAPIAVRACVTVIGGAIGPALIPVLRDT
                     GLTELGYALGLLLGYLLA"
     gene            complement(6391..6657)
                     /locus_tag="VH419_13670"
     CDS             complement(6391..6657)
                     /locus_tag="VH419_13670"
                     /inference="COORDINATES: protein motif:HMM:NF025378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4229 domain-containing protein"
                     /protein_id="HEY7044712.1"
                     /translation="MRAFVVYTLARLGLFAGVYGLIWLVFGHWIEWNALSALYTAIIA
                     LVVSSLLAFAFLKSLREDLAKHVQQRADKAKAAYESRRSREDDN"
     gene            complement(6654..7607)
                     /locus_tag="VH419_13675"
     CDS             complement(6654..7607)
                     /locus_tag="VH419_13675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009084045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HEY7044713.1"
                     /translation="MTAAADRIAGAVRRTPLIRADWGRSDAPLWLKAESLQVGGAFKA
                     RGATNAIALLSPEEKASGVITHSSGNHAQAVARAARDAGVSATIVMPHQSPAVKRAAT
                     EALGATVVLVDIAERAAEVERIRARTGAVFVPPFDDRRIIAGQGTVGLEIATDLPDVE
                     TVFVPVSGGGLISGIAVAIKACCPEARLVGVEPELAGDLADGFARGEHVVWDTDRTGR
                     TIADGLRVTGVGELNWLHITALVDDVVTVTEQQILGALRRIVLDSKLVAEPSGAVAAA
                     GYLAHPDLCRAGPAVAIVSGGNVEPAVLCDVVSRSGGGSSS"
     gene            complement(7652..7939)
                     /locus_tag="VH419_13680"
     CDS             complement(7652..7939)
                     /locus_tag="VH419_13680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013861667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLD nuclease N-terminal domain-containing
                     protein"
                     /protein_id="HEY7044714.1"
                     /translation="MRYLPAILMIVLAIYSWVEIAMSDPTQVRQLPRWVWTLVVFVPL
                     LGAICWLVLGRPNGSTVPQPVPKPKPRVVAPDDDPDFLRSLRHRKPPEDQA"
     gene            complement(8009..9007)
                     /gene="ccsB"
                     /locus_tag="VH419_13685"
     CDS             complement(8009..9007)
                     /gene="ccsB"
                     /locus_tag="VH419_13685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020579515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HEY7044715.1"
                     /translation="MTETFAHLSNNAMYSATIVYALAMLAHVVEWAKARDLPPTPSQT
                     LVRSAANVSASAVPSPPGLHDPAKGAEPTEDEIERTDRWGRIGFSLTVLGMLLQAVGV
                     VTRGIAAQRVPWGSMYEFAITGSLAVVVAYVLLVRRYAIRWLGLFVTGFLVTVLGIAM
                     IVLYRPVGPLVPALHSYWLAIHVSAAAVAGGAFTIGALASVLFLVRSRMEAKAVAHPE
                     RPQTGFLWRAPSSESIDRVAYRVHAFAFPLWTFAVLAGAIWAEYAWGRFWGWDPKEVW
                     AFITWVAYAAYLHARATAGWRGRAAAYVALLGYATFLFNFVGINLFGSGLHSYSGM"
     gene            complement(9004..10611)
                     /locus_tag="VH419_13690"
     CDS             complement(9004..10611)
                     /locus_tag="VH419_13690"
                     /inference="COORDINATES: protein motif:HMM:NF016995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HEY7044716.1"
                     /translation="MSQSSDTKVRPPEPPPPTTPPPLRPVEFLRWIWRQLTSMRTALI
                     LLFLLAVAAIPGSLIPQQRVDPSAVVAFKDRHPDLAPLFDRIGMFTVYSSVWFSAIYL
                     LLMVSLVGCFVPRLLVYAKASRAKPPEAPRNLTRLSAYDTWTTDDPPTVVAEQARAVL
                     AGQRRRTIVYPGTDQAVVAAEKGYLREAGNLLFHGALLVVLVGVAITGLYGFKGSAAV
                     ITGDGFSNTLIQYDDFTPGARFDPADLAPFSFTVDDFHVTWKKDGAGAGTPLTFDADL
                     SVVDKPGDPAHSYDLRVNHPLKVDGTSVYLVGHGYAPRVTVRDGDGRVAFSGPVIFLP
                     QDSSFISYGVVKVPDAAPEQLGFEGYFFPTASLCDGQPCSTFPDADNPVLSLVAYRGN
                     LGIDDGAAQSVYVLDKDKLKPFTTPGGKPRALLLREGQTVSLGNGAGSVTFDGYDRWV
                     KLQINRAPAKVVPLVGVLLAIVGLLGSLFVRPRRTWVKVRPVDGRTVVEVAALDRVSG
                     GDPAAHVRDVAARLRAGTRRGDGEDQE"
     gene            complement(10595..11416)
                     /locus_tag="VH419_13695"
     CDS             complement(10595..11416)
                     /locus_tag="VH419_13695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012923882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis CcdA family protein"
                     /protein_id="HEY7044717.1"
                     /translation="MAVPMASLDTMQAVHALPAASVASWFHDTALDGPVLLAVPVALT
                     AGLVSFFSPCVLPLLPGYLSYITGLSGADIVTNGSTGVRGRMVTGTLLFILGFSFVFV
                     AVGGAFGQIGYELIEHQQALTKILGVVTIVLGLVFMGVVPWMQRDVRIHAVPAVGLGA
                     APLLGVLFGLGWTPCIGPTLGAVLSLSGLGGSPTRGAFLAFVYCLGLGIPFFVAAVSY
                     RKMMSAVGWARRHQLAIMRFGGGMLVVVGVLLVTGAWDAMIVHMKDWVGDYVPVV"
     gene            complement(11423..12019)
                     /locus_tag="VH419_13700"
     CDS             complement(11423..12019)
                     /locus_tag="VH419_13700"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEY7044718.1"
                     /translation="MSARRPSRLATTLRCGVLVAALTAASACTTAGSDATGDNGYVSG
                     DGSITVIDPADRKPAPTIQGPKLGGGTLDTSAYADETVVLNIWGSWCGECRGEAADLA
                     AASKLLRKDGVVFVGLDTRDRDSLAEAYVRRFDIPYPSLVDQDSRLLLDFYGIVSPNA
                     IPSTLVIDSDGKLAAVITGPTTKDTLVDLVGDVQQSTS"
     gene            complement(12021..12734)
                     /locus_tag="VH419_13705"
     CDS             complement(12021..12734)
                     /locus_tag="VH419_13705"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY7044719.1"
                     /translation="MTTRSEDTPGTDVTPSVASGHDAPTTVIHLLRHGEVDNPKGILY
                     GRLPGYHLSELGRQMAERVAKTLSGRDITHLVSSPLERAQETARPSADALGLEIVSDK
                     RVIEAENAFEGLRFGVGDGSLRKPSAWKHLYNPFRPSWGEPYKDVVVRMLAAMHDARE
                     TATGHEALIVSHQLPVWIVRSFVEGRRLFHDPRKRECSLASLTTFTYSGDRVTTVSYS
                     EPAADLVPVRARKKFTAGA"
     gene            complement(12731..14059)
                     /gene="hemL"
                     /locus_tag="VH419_13710"
     CDS             complement(12731..14059)
                     /gene="hemL"
                     /locus_tag="VH419_13710"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020392281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEY7044720.1"
                     /translation="MSTALFERAVKVTPGGVNSPVRAFRAVGGTPRFMAHGSGAYVYD
                     VDGNEYVDLVCSWGPLILGHAHPAVVDAVAAAVSRGTSFGTPSRPEVELAEEIVARTP
                     VEQVRLVSSGTEATMSAIRLARGFTGRDLVVKFAGCYHGHVDSLLAAAGSGLATFAVP
                     GTPGVPAASTASTIVLPYNDLASVEAAFAAYGDQIACVITEAAAGNMGAVAPADGFNA
                     GLARLCREHGALFVSDEVMTGFRVSRSGYWGLDGMVEGWTPDLMTFGKVMGGGFPAAA
                     FGGRADVMAHLSPLGPVYQAGTLSGNPIATTAGLATLRQATDAVYARLDETAATIGRL
                     TGEALTSAGVPHVIQYAGNLFSTFFLDPTTTPVVRNFDDASRQAPDVFRAFFHAMLEH
                     GVYLPPSGFEAWFVSAAHDDPAVDRIASALPVAAAAAAAALHSEEGHGAP"
BASE COUNT         2319 a         5036 c         5010 g         2075 t
ORIGIN      
        1 ctagaagagc gcggggtgct cctgagagag ccagaagccg tggtcggact cgaggtggta
       61 gcagcggatc ccggactgac ggctcgtgca cccgaagtca ccgacgacgg tggacgtccc
      121 gtagtccagt accggcgctc ccgggttgcg caccgtgtcg gtcacgcaga ggaacgacgc
      181 gatgccgtgc gccgtcacgg cgatgctctg accgtagtcg ccgtgacagc ctgccggttt
      241 gggcggatcc cggtagacgt gcttggcgat gtcgcagcga gcctgaccgg caccgatggc
      301 acagccgatg ttgcgggacg gggtgaggaa caccggcacc ccgccgatga tgttggggag
      361 gtggcgccgc gctgtctgcg gtgggcgcgg cgactgcgag gacccggtcg ggcccgatgt
      421 cgtcgaaggc ggtgtggact ccagcggtgt ggaagggtcc gacgtggtcg gtgcctgtgg
      481 cgtcgagggc cgttcggtgt tcgtctggtg tacggcgtcg gtacgtgcca ccgatgcgac
      541 gtcctgtgcg gaggcgcagc cggcgaggca actgacggca agtgcaacgg ccgccaaccg
      601 ccctgcccgc acgtgaacct cccacgtcgc tgtgtctcac gcaccgtccg gtgcggatgg
      661 gacccgatga tggcacacgg ccgccgtgcg aggtcctgcg agagcagtcg gtgacgcggg
      721 gcgagagcag tcaggcggat cccctgtgtc aggcaggttc ggcgacccag cgcatcgggt
      781 cgccccagcc gaggtcgctg atgccgagcg cgtcgagtgc ttgcacggcg agcgtgcgcc
      841 ggtacgccgc gaacgtgagc acgtgcgcga tcatgccgcc gtacgtgaac acctcgggcg
      901 ggtcgcacac cgcgtcgacg aacgtctcgt ccagcctgcc ttgctcgcac acctcacgga
      961 tgtgaccgag atagaccggc cctactcgat ccagccgcgt ccgcatcgca gccgcgggct
     1021 cgtgctgctc cacgtcccag tcgtacgcct cgttggcgat ggcgcagttc cacatgtcca
     1081 tctgcccgat cagcctcgac aggagatggc gcaaggacgt gacgtcgtca ccttcgtcga
     1141 cggagacctg gatcggccgg tcgagtccgt cgcctggtga cgcagcgtcg tcgagccggt
     1201 tcgccctgtc gacgagctcg ccgacgagcc acacatggtg ctcgaccatc ttgacgagaa
     1261 gatccatgga actgacctcg attcgggcag gcagccgaag gccgccaggt ggatggaaat
     1321 gcacgccgtt cggggagtcg agctggatgc gccggtgcgg tttgctccgc cagcgggccg
     1381 gcccgacgcc gtacgcccgt tggaacgcac gggtgaacgc ctcgttgctg gagtagcccg
     1441 cctcgacggc gatgtcgagg actcctcgcc cactcgtcaa caaccggtac gccgaccgtt
     1501 cgagcagcac ccgacgacga aaccgcgccg gggtctcacc ggcggtcgcc cgcaccacac
     1561 ggtcgaagtg gaagcgcgac agataggcac gcgcagccag gtcatcgccg cgcacctcgt
     1621 ggtcgtcgag gcagccagcc agcgtgtcga cgaaccgtac gaaggtgtcc ggctgcgcgt
     1681 tcatgtcgcc agtgtcactt ccaccgacgt cttcacgctt gatcgttctt gcgcaggttc
     1741 tgtcacgaca cgagggcctt cagctcctcg ccgagcgcac gacgccgatc acgcgacaac
     1801 ggcacctcga gcaccctgac tcccgtcgcc ggttccacca gggcagcctg caacctgtcg
     1861 atggtctcga tccgttcgta cgccgtccgc gtgctcgcgc acaggctcgc gagatccacc
     1921 gcatgcggcg taccgaagac gcgttcgaac gcatcggcgt actccggagc accctgctcg
     1981 agcgtggtga agatcgcgcc gccgtcgtcg ttcgcgacca cgatcgtcag gtccggccgt
     2041 ggctcgtccg ggccgagaac gagaccgttg gcgtcgtgca ggaacgtcag gtccccgacg
     2101 taggccagcg accgagccga ccgacggccc agggccgcgc ccactgccgt cgagacagtg
     2161 ccgtcgatgc ccgacagccc acggtttccg acgatgagcc ggcgttctcc cgacgggtac
     2221 ggcgtcgtca tgatgtccag gtcgcggacc ggctgcgacg agccgaccac cagcagtcca
     2281 gccggcggga cggactccgc cacggcgccc gcgacccgca agggcagccc ttcgtctcga
     2341 gccgcgatcg agtccacgcg ggcagacagc gctgcgtcgg ccgaacgcca gcggtccatc
     2401 catgcctcgt catccggctt ctcgactgcg ggcaccgcgt cgacatgccg ggccacccga
     2461 ccgggatcgg tgcagacacc accacgtccc cgcacggaga tcacctcgat gtcggtgcgg
     2521 gagatgagcc gggtcatcgg cctcgacagc gtggggtgtc ctgtgacgac gacccgttcg
     2581 acgtcgtcgg cgagactgcc ggcgagcagc aaccgcgacg tgcgcatcgc gtgcgtgccg
     2641 gtgcgagcac ccgacgtggg ctctgccagc agcggccagt tggccgtctc tgcgaggagg
     2701 cgtgccggcg gccccgcgtc gtcgcccgcc acgacgaccg tacgcagtcc gggcgagagg
     2761 agctcggagt ccggatccgc gtctcggctc caccggcgtc gcgcctgtcg tcgagaaggc
     2821 ggctccgcgg cgagcgctgc ggagacctgc gcggggtcgg ggagcaaggg ttcggcgaac
     2881 tgcaggttga cgtgcgaagg gcccgaacgg cgtaccgcct ccgcgatcgc ctcgaccgct
     2941 tgtgtccgcg catcgacggc cacatcggtg cacggcacca ccgagccgaa gatgcccacc
     3001 tggtcggttg tctggttggc accagtaccc ctcagcaagg ccggccggtc ggccgacagc
     3061 acgatgaggg gctccccggc gtgccatgcc tccagcactg ccggatgcag gttggcgacc
     3121 gcagaacccg acgtcgtggc aaccgccacc ggtcggtgac tgcccttcgc cacgccgagc
     3181 gccaggaatc ccgccgttcg ctcgtcgatg cggacatgca accgcagcag gccgcgccgg
     3241 tcagcctcgt ggaacgcgat cgcgagcgcg gccgagcgag agcccggcgc gaggacgacg
     3301 tcgtggacac caccacgacg gagcgtgccg acgacgtcga ctgccaggtc accggacgcg
     3361 ctcatcgccg atccgcttcc agcacggctg cgcacgcggc cagtcgatgg tgccagcgtc
     3421 cggcggtcgc gtcgtctgct cgcaccgcgt cgagcgccgc ggggtccgct gcgatccggc
     3481 gtacgtcgag catgccctcc accggcgtga gcggatcgtc gaccacgtcg gcggtgaaca
     3541 tcgccgtggt cgcgagcccg catgcgtacg gaagttctgg caacgcagcg gccagcgcga
     3601 cccccgccgc gatgcccacc gatgactcga gcgccgacga gacgacgacc ggcatcgaga
     3661 tctgctcggc gagtcgcagg caggcccgca caccgccgag cggctggacc ttgagcaccg
     3721 cgatgtcggc ggcggcgagc tgcttcacgc gcagcgggtc ggaggcgcgc cggatcgact
     3781 cgtccgcagc gatggccacg tcgacgcgac gcctcaccag tgccaggtcc tccacggttg
     3841 cgacgggctg ctcgacgtac tccaggtcga actcggcaag ccctgcgatc gccttgaccg
     3901 cgttgtcgac gggccaaccg ccgttcgcgt cgacgcggat ccgtccgccg ggcccgagtg
     3961 cctcgcgcac cgccgcgacg cgggcgacgt cgtcgtcgag cgtctggccg ggctcggcca
     4021 ccttgacctt cgcggtacgg cagccgtgcg atctcgccac gatctccttc gcctcgtcag
     4081 gcgtcacggc gggaacggtg cagttgaccg gtaccaggtc gcggagtggc gtcggaaacg
     4141 gctggtcggc cgcctccagc gcagcagcga gccacgcggc ggactccgtc gcgtcgtagt
     4201 cccagaacgg gctgaactcc ccgacccctg ccgctccctt gatcagcatg ccttcgcgcc
     4261 gccgcagccc tcggaaccgc acgttcatcg gcagcgagaa caccgccacc tcacgagtcg
     4321 tcccacgcag cacgggtggt ctgctccgca acgggtcgga cgaaccgagg gtggggaggc
     4381 gtcgcgtgtc gactttgccg gagctgagca tcggcagctc gtcgacgacg acgaggctgc
     4441 gtggcgccca ctcacgggga tggacctcgg cgacgaaatc gcggatggcg tccagcgtcg
     4501 ggagcgccgg tgcccgagct gccacagcca cgacctcgac gccccactca gggtccggtc
     4561 gagacgtgat cgcgacaccg gtgaggcccg gcatctcctg cagacgcgac tcgaccaccg
     4621 agagcggcac gttcacgcca ccggtgatga cgacgccgtc gcttcggccg agcacgacca
     4681 gccggccgtc gtggtcgagc ccgccgatgt ccgacgtgtg gaaccagccg tcgcgcaaga
     4741 cggtctcggt caggtcgggt cgttcggcgt acccgtcgaa cagcaccgga ccgctgagcc
     4801 tgatctcacc cgccgacccc agcgccaccg cgacaccgtc gagtggcacg ccgtcgtaca
     4861 cacagccgcc acaggtctcg ctcatgccgt acgtcgtcac gacgttgacg ccggcttcgc
     4921 gggcccggtc gagcagcgcg tccggagccg ctgcgccgcc gaggagaacg acgtcgtacg
     4981 cagcaagcga gttggcgtcg gagaccaaca gccggtgcag ctgcgtcggg accagtgcgg
     5041 cgtagcgacg ctcgccggtc acggccgccg tcgccgtcgc gacgtcggga tactcctcca
     5101 gcacgaccgg cgacgtggcg gccagcaggc tacggacgag gacctgcaga ccggcgacgt
     5161 actgcggcgg caacgccagc agccactggc caggcccgcc gatcctcgtc agcgtggcga
     5221 gcgcggaggc gcgaacggcg gaggcggaca gcaggacctc cttgggttca ccggcggatc
     5281 ccgacgtgcg aaccgtcaac ggcgacccgg cttcgtcgcc gagccaacgc ccgagcagcc
     5341 gttcgagcgc gcgagcgtct ccgctgagcg gccgcaacga ggcctcgtcg gtcacgccgg
     5401 acagactacg gccgacgcca cgacgtcccg atgcggctgg cagactgtcc gcgccatgac
     5461 gaccgccacc accaccgcgg cccactggat cgagggggcc cgaccgcgca cgttgcccgc
     5521 tgccgtttca cccgttctcg ccggcaccgg ctccgccgtc tacgccgaca gcttcgtctg
     5581 gtggaaggcg gtgctggcac tcgtcgtcgc cctcgcgctc caggtcggtg tcaacttcgc
     5641 caacgactac tccgacggga tcagaggaac cgacacgaac agggtcggtc cgttgcggct
     5701 cgtcggctcc gggctggtcg cggcgtctcg ggtcagagct gccgcgttcc tcgccttcgc
     5761 cgttgccgga gtggcaggtc ttgtgctggc cgcgacgacc acgtggtggc tgttgctcgt
     5821 cggcgctgtg gcgatcgtcg ccgcctggtt ctacaccggc ggctcgcggc cgtacggcta
     5881 ccacgccctc ggtgaggtga gtgtcttcgt cttcttcggc ctggtggccg tcgtcgggac
     5941 ggcgtacgtc cagaccgagg acgtcacgct cgacgcggtt ctggcgggct gcggggtcgg
     6001 tgctctggcc tgcgcgatcc tcgtcgcgaa caacctgcgc gacataccaa ccgaccgcga
     6061 ggcggggaaa cagacactgg cggttctgct cggcgaccat cgcgcccggg tgctgtacgt
     6121 cgctttgctg gctgcggcgc tcctgctcgc cgtcgccctg gctgtgtcca cgtcggcgtg
     6181 gctgctgctg gcgttcgtgg cggcaccgat cgccgtccgg gcatgcgtca cggtcatcgg
     6241 cggcgcgatc gggccagcgc tcatccccgt cctgcgcgac accggcctca ccgagctcgg
     6301 ctatgccctc ggcctcctgc tcggctacct cctcgcttga ccgagttgtc agaaccccat
     6361 ggagtcatag gtcgatggag ttctgacaac tcagttgtcg tcttcgcggg agcggcggga
     6421 ttcgtacgcc gccttggcct tgtcggcgcg ttgttgcacg tgcttcgcga ggtcttcgcg
     6481 caacgacttc aggaaggcga aggccagcag ggaagagacg accagcgcga tgatggcggt
     6541 gtagagagcg ctcagcgcgt tccactcgat ccagtgcccg aagacgagcc agatgaggcc
     6601 gtagacgccc gcgaacaggc cgagccgcgc caacgtgtag acgacgaaag ccctcatgag
     6661 ctggacccgc ctcccgaccg gctcaccacg tcgcagagga cggccggctc cacgttcccg
     6721 cccgagacga tggccaccgc cggaccggca cggcacaggt ccgggtgcgc gagatagccg
     6781 gcagccgcga ccgccccgct cggctcggcg accagcttcg agtcgagcac gatgcggcgc
     6841 agggcgccga ggatctgctg ctcggtgacg gtgaccacgt cgtcgacgag cgccgtgatg
     6901 tgcagccagt tcagctcacc gaccccggtg acccgaagac cgtccgcgat cgtcctgccg
     6961 gtgcggtcgg tgtcccagac cacgtgctca ccacgcgcga agccgtcggc aaggtcgccg
     7021 gcgagctccg gctcgacacc gacgagacgg gcctccgggc agcacgcctt gatcgcgacc
     7081 gcgatcccac tgatgagccc accgccgctc accggcacga acacggtctc gacgtcgggc
     7141 aggtccgttg cgatctcgag cccgacggtt ccctgccccg cgatgatccg acggtcgtcg
     7201 aacggtggca cgaacaccgc acccgtcctg gcccggatgc gctcgacctc cgccgcgcgt
     7261 tcggcgatgt cgacgagcac gacggttgcc ccgagcgctt cggtcgcagc gcgtttgacg
     7321 gcaggagact ggtgtggcat cacgatcgtg gccgacacgc ccgcatctcg tgccgcccgg
     7381 gccaccgcct gggcgtggtt gccggaggag tgcgtgatga ccccgctggc cttctcctcc
     7441 ggcgaaagca gggcgatcgc gttcgtcgct ccccgcgcct tgaacgcgcc gccgacctgc
     7501 agcgactcgg ccttgagcca caacggggca tccgaccgac cccaatcggc ccgtatcaac
     7561 ggcgtacgac ggacggcgcc ggcgatccgg tcggcagcgg ccgtcacgtc ctggagtgag
     7621 accagcgtcg acgtgtcctg cgtgacgggg ttcaggcctg gtcctccggc ggcttgcggt
     7681 ggcgcaggct gcgcaggaag tccgggtcgt cgtcgggtgc gaccacacgc ggcttcggct
     7741 tcgggaccgg ctgcgggacg gtgctgccgt tcggacgtcc cagcaccagc cagcagatgg
     7801 cgcccagcaa ggggacgaac accacgagcg tccagaccca gcgaggcagc tggcggacct
     7861 gtgtcgggtc cgacatcgcg atctctaccc agctgtagat cgccagcacg atcatcagga
     7921 tggcgggtag atatctcatc gcgtggcctc ctcttctccc tccagagtaa acgcgtggtc
     7981 caagccgcgc gccatcccgg tcaggccgct acatcccgga gtacgagtgc agcccggagc
     8041 cgaacaggtt gatccccacg aaattgaaca ggaacgtcgc gtacccgagc agcgcgacgt
     8101 acgccgccgc gcgtccgcgc caccctgcag tcgcgcgtgc gtggaggtac gccgcgtagg
     8161 cgacccaggt gatgaacgcc cacacctcct tggggtccca gccccagaag cgaccccagg
     8221 cgtactccgc ccagatcgcg ccggcgagca ccgcgaacgt ccacagcggg aaggcgaagg
     8281 cgtggacccg gtacgccacc cgatcgatcg actccgacga cggcgcgcgc cacaggaacc
     8341 cggtctgcgg ccgctccgga tgtgcaaccg ccttggcctc catccggctc cggacgagga
     8401 agaggaccga ggcgagcgcg ccgatggtga aggcgccgcc ggcgacggca gcggcggaca
     8461 cgtggatcgc caaccagtag gagtgcagcg ccggcaccag cggtccgacc gggcggtaca
     8521 ggacgatcat cgcgatcccg agcacggtga ccaggaaccc ggtgacgaac aggccgagcc
     8581 agcggatggc gtagcggcgt acgagcagga cgtacgccac gacgaccgcc agtgacccgg
     8641 tgatcgcgaa ctcgtacatg ctgccccacg gcacccgttg cgcggcgatg ccacgagtca
     8701 cgacgcccac ggcctgcagc agcatgccca agacggtgag cgagaaaccg atccgccccc
     8761 acctgtcggt ccgctcgatc tcgtcctccg tcggctcggc acccttcgcc ggatcgtgga
     8821 gtccaggcgg agacgggaca gccgacgcgg agacgttcgc ggcgctgcgc accagtgtct
     8881 ggctcggggt gggaggcagg tcgcgggctt tcgcccattc gacgacatgg gccagcatgg
     8941 cgagggcgta cacgatcgtg gcggagtaca tcgcgttgtt gctcaggtgc gcgaacgtct
     9001 cggtcactcc tggtcctctc cgtcacctcg gcgagtgccg gcgcgtagcc gggcggctac
     9061 gtcgcggacg tgagccgcgg ggtcgccgcc tgacacccgg tcgagcgcgg cgacctcgac
     9121 caccgtacgc ccgtcaaccg gccgcacctt gacccaggta cggcgcggcc ggacgaacaa
     9181 cgacccgagc agacccacga tggcgaggag cacacccacc agcgggacga ccttggccgg
     9241 cgcccggttg atctgcagct tgacccagcg gtcatagccg tcaaaggtga ccgagcccgc
     9301 gccgttgccg aggctcacgg tctgtccttc gcgcagaagc agcgctcgtg gcttgccgcc
     9361 cggtgtcgtg aacggcttca gcttgtcctt gtcaagcacg tacaccgact gtgcggcacc
     9421 gtcgtcgatg ccgaggttgc cgcggtaggc gacgagcgac agcaccgggt tgtccgcgtc
     9481 cgggaacgtc gagcagggtt gtccatcgca cagcgaggcg gtcgggaaga agtacccctc
     9541 gaaccccagc tgttccggtg ccgcgtcggg aaccttgacg actccgtacg agatgaacga
     9601 gctgtcctgc gggaggaaga tcaccggccc ggagaacgcc actcggccgt cgccgtcacg
     9661 cacggtcacc cgcggcgcgt agccatgacc cacgaggtag accgacgtgc cgtcgacctt
     9721 cagcgggtgg ttgacccgaa ggtcgtacga atgggccggg tcgccaggct tgtcgacgac
     9781 cgacaggtcg gcgtcgaacg tcaacggggt gccggcgccg gcaccgtcct tcttccacgt
     9841 gacgtggaag tcgtcgacgg tgaacgagaa cggcgccagg tcggcaggat cgaatcgggc
     9901 gccgggcgtg aagtcgtcgt actggatcag cgtgttggag aaaccgtcgc cggtgatgac
     9961 agcggcgctg cctttgaagc cgtagaggcc tgtgatcgcg acacccacca gtaccacgag
    10021 cagcgcgccg tggaagagca gattgccggc ctcccggagg tagcccttct ccgcagccac
    10081 gaccgcctgg tccgttcccg ggtagacgat ggtgcggcga cgctgtccgg cgagcacggc
    10141 acgggcctgc tcggccacga cggtgggcgg atcgtccgtc gtccaggtgt cgtacgccga
    10201 cagacgtgtc agattgcgcg gcgcctccgg tggcttcgcc ctcgacgcct tcgcgtagac
    10261 cagcaggcgc ggcacgaagc acccgacgag cgacaccatc agcagcaggt agatcgcgct
    10321 gaaccagacc gagctgtaca cggtgaacat gccgatccgg tcgaacagcg gagccaggtc
    10381 gggatgccgg tccttgaacg ccacgaccgc tgacgggtcg acgcgttgct gcgggatgag
    10441 cgagccgggg atcgccgcca ccgcgagcag gaacagcagg atgagcgcgg tacgcatcga
    10501 cgtgagctgt cgccagatcc agcgcaggaa ctccaccggg cgcagcggcg gaggcgttgt
    10561 cggcggcggt ggttcgggcg gccggacctt ggtgtcagac gactgggaca tagtcaccca
    10621 cccagtcctt catgtgcacg atcatcgcgt cccaggcgcc ggtgacgagc agcacgccaa
    10681 cgacgacgag catcccgccg ccgaaccgca tgatggcgag ctggtgacgc ctcgcccagc
    10741 cgacggcact catcatcttg cgatacgaca cggcggcgac gaagaagggg atcccgagac
    10801 cgaggcagta cacgaatgcc aggaacgctc ctctggtggg gctgccgccg aggccggaca
    10861 gcgagagcac cgcacccagc gttgggccga tgcagggcgt ccatcccagg ccgaagagga
    10921 caccgagcag gggagcagca ccgaggccga cggcggggac ggcgtggatg cgcacgtcac
    10981 gctgcatcca cggcacgaca cccatgaaga cgagcccgag gacgatcgtg accacgccga
    11041 ggatcttggt cagtgcctgc tggtgctcga tcagctcgta cccgatctgc ccgaacgccc
    11101 cacccaccgc gacgaagacg aacgagaagc cgaggatgaa caggagggtc ccggtcacca
    11161 tccgcccacg aacacccgta ctgccattcg tcacgatgtc ggcgccggaa agcccggtga
    11221 tgtaggagag gtagcccggg agcagcggca gcacacaggg cgagaagaac gagacaagac
    11281 ccgcggtcag ggccaccggc accgcgagca gaacgggtcc gtcgagtgcc gtgtcgtgga
    11341 accaggacgc caccgacgca gccggcagtg cgtgcaccgc ttgcatcgtg tccagtgacg
    11401 ccatggggac ggccacgacg gctcagctcg tgctctgctg gacgtcgccg acgaggtcga
    11461 ccagggtgtc cttggtggtg gggccggtga tcacggcagc cagcttgccg tcgctgtcga
    11521 tgacgagcgt ggacgggatc gcgttcggcg agacgatgcc gtagaagtcc agcagcagcc
    11581 tgctgtcctg gtcgaccagg ctcggatacg ggatgtcgaa acggcgtacg taggcctctg
    11641 cgagcgagtc ccggtcacga gtgtcgagac cgacgaagac cacaccgtcc ttgcggagca
    11701 gcttcgacgc cgccgcgagg tcggcggcct cgccccggca ctcgccgcac caggatcccc
    11761 agatgttgag caccacggtc tcgtcggcgt acgccgaggt gtccaacgtt ccgccaccca
    11821 gcttcgggcc ctggatcgtc ggggcaggct tgcggtcggc cgggtcgatc accgtgatgg
    11881 acccgtcccc gctgacgtag ccgttgtcac cggtcgcgtc ggacccagcg gtcgtgcatg
    11941 cgctcgccgc ggtcagcgcg gcgacgagca cgccacaccg cagcgtggtg gcgagccggc
    12001 tcggtcgacg tgcgctcacc tcaggcgcct gcggtgaact tcttcctggc ccggaccggg
    12061 accaggtctg ccgccggttc ggagtacgac acggtcgtga cccggtcgcc ggagtacgtg
    12121 aaggtcgtca ggctcgccag gctgcactcc cgcttgcgcg ggtcgtggaa gaggcggcgg
    12181 ccctcgacga acgaccgcac gatccacacc ggcagctggt gagacacgat gagcgcctcg
    12241 tgccccgtgg ctgtctcgcg cgcgtcgtgc atggcggcga gcatccgcac cacgacgtcc
    12301 ttgtagggct cgccccagga agggcggaac gggttgtaca ggtgtttcca cgccgacggc
    12361 ttgcgcaacg agccgtcgcc gaccccgaag cggaggccct cgaaggcgtt ttcggcctcg
    12421 atcacacgct tgtcgctgac gatctcgagc ccgagcgcat cggccgacgg ccgcgccgtc
    12481 tcctgcgcac gctcgagggg tgacgagacc aggtgggtga tgtcgcgacc ggacagcgtc
    12541 ttggcgacgc gctccgccat ctggcggccg agctcgctga ggtggtagcc gggaagccgg
    12601 ccgtacagga tgcccttggg gttgtcgacc tcaccgtgcc ggagcaggtg gatgaccgtg
    12661 gtgggtgcat cgtggccgga cgcgacggac ggcgtcacat cggtaccggg cgtgtcctcc
    12721 gaccgggtcg tcatggggca ccgtgccctt cctcggagtg cagggcggct gcggcggctg
    12781 ccgcggccac ggggagtgcg ctggcgatcc ggtcgacagc cgggtcgtcg tgtgcggcac
    12841 tgacgaacca cgcctcgaac ccgctcggcg gcaggtagac gccgtgctcg agcatcgcat
    12901 ggaagaaggc gcggaacaca tcgggagcct gacgactcgc gtcgtcgaaa ttgcgtacga
    12961 cgggggttgt cgtcggatcc aggaagaacg tgctgaacag gttcccggcg tactggatca
    13021 cgtgcgggac accagcactc gtcagcgcct cgccggtgag tcgtccgatg gtggccgcgg
    13081 tctcgtcgag ccgggcgtac acggcatcgg tggcctgccg cagggtcgcg agcccggccg
    13141 tcgtggcgat ggggttgccg gacagggtgc cggcctggta gacggggccg agcggcgaca
    13201 ggtgggccat gacgtcggcg cggccgccga acgcggcggc cgggaagccg cctcccatca
    13261 ccttgccgaa cgtcatcagg tcaggcgtcc agccctcgac catgccgtcc agcccccagt
    13321 agccggacct ggagacgcgg aagccggtca tcacctcgtc gctgacgaac agcgccccgt
    13381 gctctcggca cagccgcgcc aggccggcgt tgaacccgtc tgcgggtgcg accgcgccca
    13441 tgttgccggc ggcggcttcg gtgatcacgc aggcgatctg gtcgccgtac gccgcgaacg
    13501 ccgcctctac cgaagccagg tcgttgtagg gcagcacgat ggtggacgcc gtggatgctg
    13561 ccgggactcc gggcgtgccc ggtacggcga acgtcgccag gccggacccg gcggcggcga
    13621 gaagggagtc gacgtggccg tggtagcagc cggcgaactt cacgacgagg tcgcgcccgg
    13681 tgaacccgcg agccagccgg atggccgaca tcgtggcctc ggtgcctgac gagacgagcc
    13741 ggacctgctc gaccggcgta cgggcgacga tctcctcggc gagctcgacc tccggcctcg
    13801 acggtgtgcc gaaggacgta ccgcgcgaga cggccgccgc cacggcgtcg acgactgccg
    13861 ggtgagcgtg gccgaggatg agcgggcccc acgagcagac caggtcgaca tactcgttgc
    13921 cgtccacgtc gtacacatag gcgccggagc cgtgcgccat gaagcgcggc gtcccaccga
    13981 ccgcccggaa ggcgcgcacc gggctgttga cgccgccggg cgtcaccttc accgcacgct
    14041 cgaacagggc cgtcgacacg ggggtgacag gtccgccggc ggccacttca gcgtgcagct
    14101 cggcatgttc cagggaagtc accgccccat tgtcaccgag ccggccaagc ggccacgaat
    14161 cggcctcggg gtgtgggcga cctggggcgt ggtgcccggg tacgtccccc gctggtctgc
    14221 cgtcaggacg ggaccgccgt gacgtgcggc ggcgctcctg cagctgctga tccgactttg
    14281 gtccagccga cctggccccg ccgccaccgg tggacccgcc ctgcctcccg gtcgcctgca
    14341 ccgactgacg gtcccggcga ggagtctgca cggcagaccg cctgcttgta tcgagggttt
    14401 gcgatgttta cacgcctttt gggctgtaaa catcgcaaac
//
LOCUS       DATBFZ010000450         1901 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_4250201, whole genome shotgun sequence.
ACCESSION   DATBFZ010000450 DATBFZ010000000
VERSION     DATBFZ010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1901
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4250201"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1901)
                     /locus_tag="VH419_13715"
     CDS             complement(<1..>1901)
                     /locus_tag="VH419_13715"
                     /EC_number="4.1.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009082910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional oxoglutarate
                     decarboxylase/oxoglutarate dehydrogenase thiamine
                     pyrophosphate-binding subunit/dihydrolipoyllysine-residue
                     succinyltransferase subunit"
                     /protein_id="HEY7044721.1"
                     /translation="DYAGTTISLTNPGGIGTVHSQPRLLAGQGTIIGVGAMEYPAPFA
                     GMSDKQLAELAVSKIITLTSTYDHRIIQGAQSGEFLKVMHELLLGEQGFYDSIFTSLR
                     IPYEPVRWVRDVSQGAEGQIDKTARVIELIHAYRVRGHLMADTDPLEFRMRRHPDLDV
                     LQHDLTLWDLDRTFPVGGFAGQQKMKLREVLGVLRDSYCRRVGVEYMHIQAPEERRWI
                     QERVERRYEKPTVDEQRHILGRLNAAEAFETFLQTKFVGQRRFSLEGGESLIPLLDEV
                     LGNSATRGLDEVVIGMAHRGRLNVLANIVGKPYEKIFNEFEGHLDPKMAHGSGDVKYH
                     LGMDGKFTTADDQHTIAVSVVANPSHLEAVDPVLEGIVRAKQDRLDLGLEGYSVLAVA
                     IHGDAAFAGQGVVAETLNLSQLRGYRTGGTVHVIVNNQVGFTTAPEYSRSSLYSTDVA
                     RMIQAPIFHVNGDDPEAVVRVARLAFEYRMAFNKDVVIDMICYRRRGHNEGDDPSMTN
                     PQMYSIIDTKRSVRKLYTEELIGRGDITLDMAEESLRDFQQQLETVFKATRDSSTVVR
                     TTRDRTVEPEPTVATAIPATLVKRIGDAHVELPAGFTPHKRVAQLLERRAKMSVEGDI
                     DWGFAEIIA"
BASE COUNT          271 a          639 c          663 g          328 t
ORIGIN      
        1 aacgcgatga tctcggcgaa gccccagtcg atgtcgccct cgacggacat cttcgcgcgg
       61 cgttcgagta gctgcgcgac gcgcttgtgc ggggtgaagc cggccggcag ctcgacgtgc
      121 gcgtcgccga tgcgcttgac cagcgtggcc gggatcgcgg tggccaccgt cggctccggc
      181 tcgaccgtgc ggtcgcgggt cgtgcggacc accgtcgacg agtcccgggt cgccttgaac
      241 acggtctcca gctgctgctg gaagtcgcgc agcgactcct cggccatgtc cagcgtgatg
      301 tccccgcggc cgatcagctc ctcggtgtag agcttgcgga ccgagcgctt ggtgtcgatg
      361 atcgagtaca tctgcgggtt ggtcatcgac gggtcgtcgc cctcgttgtg accgcggcgc
      421 cggtagcaga tcatgtcgat cacgacgtcc ttgttgaacg ccatgcggta ttcgaaagcc
      481 aggcgcgcca cccgcaccac ggcctcgggg tcgtcgccgt tcacgtggaa gatcggcgcc
      541 tggatcatcc gggccacgtc ggtcgagtag agcgaggagc gcgaatactc cggcgcggtg
      601 gtgaagccga cctggttgtt gacgatcacg tgcaccgtgc cgccggtcct gtacccccgc
      661 agctgcgaca ggttcagcgt ctcggccacg acgccctgcc cggcgaaggc cgcgtcgccg
      721 tggatggcga ccgcgagcac cgagtagccc tccaggccga ggtcgagccg gtcctgcttg
      781 gcccggacga tgccctccag caccgggtcg accgcctcca ggtgggacgg gttggcgacc
      841 accgagaccg cgatcgtgtg ctggtcgtcg gcggtggtga acttgccgtc catgccgagg
      901 tggtatttca cgtcgccgga gccgtgcgcc atcttcgggt cgaggtgccc ctcgaactcg
      961 ttgaagatct tctcgtacgg cttgcccacg atgttggcca gcacgttgag ccggccgcgg
     1021 tgggccatgc cgatgacgac ctcgtcgagg ccacgggtgg ccgagttgcc cagcacctcg
     1081 tccagcagcg ggatcagcga ctcgccgccc tccagcgaga accggcgctg cccgacgaac
     1141 ttggtctgca ggaaggtctc gaacgcctct gccgcgttca gccggccgag gatgtgccgc
     1201 tgctcgtcga ccgtcggctt ctcgtaccgg cgctcgaccc gctcctggat ccaacggcgc
     1261 tcctcgggcg cctggatgtg catgtattcc acgcccaccc ggcggcagta ggagtcgcgc
     1321 agcacgccga gcacctcgcg cagcttcatc ttctgctgcc cggcgaagcc gccgacgggg
     1381 aaggtgcggt cgaggtccca cagcgtcagg tcgtgctgga gcacgtcgag gtcggggtgg
     1441 cggcgcatgc ggaactcgag cgggtcggtg tcggccatca ggtggccacg cacccggtac
     1501 gcatggatca gctcgatcac ccgggcggtc ttgtcgatct gcccctcggc gccctgcgac
     1561 acgtcgcgga cccagcgcac cggctcgtag gggatgcgca gcgaggtgaa gatcgagtcg
     1621 tagaagccct gctcgcccag gagcagctcg tgcatcacct tgaggaactc gccggactgc
     1681 gcgccctgga tgatccggtg gtcgtaggtc gaggtcagcg tgatgatctt gctgaccgcg
     1741 agctcggcga gctgcttgtc gctcatgccg gcgaacgggg cggggtattc catcgcgccc
     1801 acgccgatga tcgtgccctg cccggccagc agccgcggct gcgagtggac ggtgccgatg
     1861 ccgcccgggt tggtcagcga gatcgtcgtg cccgcgtagt c
//
LOCUS       DATBFZ010000451         2803 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_1957058, whole genome shotgun sequence.
ACCESSION   DATBFZ010000451 DATBFZ010000000
VERSION     DATBFZ010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2803
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1957058"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2550)
                     /locus_tag="VH419_13720"
     CDS             complement(<1..2550)
                     /locus_tag="VH419_13720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012923260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY7044722.1"
                     /translation="MSVRELPTLLRDDPALLEVRGRSSALIAVPEPARAFTLAGLVAT
                     TQRRPYVIAVPTTADAERLAHDLAAYLGSDRVDLFPAWETLPFERVSPAVETMGRRLR
                     TIWRLLDPARTPDVIVAPIRALVQRLGPDIDKVEPIEVRPGERRDRDELVAALVNAGY
                     RREQQVEHRGEVGVRGSIVDVFPSTADHPVRIDLWGDEVDRLAEFSVNDQRSIADLTE
                     AQIFPCRELLPSDEVRARAGRLVSLQPWGREQWERLSDGLTFDGMESWLPWLADTEHV
                     LFDLVPADAQIVLVEPKRLRDRAADIAAEEADLARSLAKTWGADTVGEFPSLHLPFDR
                     LLVHTNASAWTVTVAPEGPDVTTIQAMGWDPVIGGGERLMSQLANLSAQGYRVVVSAD
                     GEGSKARLLRLLDEHGLKAEVAVAPLERGFILPAIKLALLADPDLTGRRRAHRPVRAR
                     RRTDAERFFDDLNPGDYVVHHQHGVARFAGMVKRSISGVERDYLLLEYRGDDKLYVPS
                     DQIDSVRHYTGGDQPSLSRLNGGDWAKTKARVRAEVTQIAQELVVLYQKRLNTPGHAF
                     PPDTPWQQELEDAFPYQETPDQLKAIVDVKADMEGSMPMDRLVCGDVGFGKTEVAIRG
                     VFKAVQDGKQAAVLVPTTLLAQQHSQTFTERFAGYPVRVEVLSRFLTPGQAKKVVDGL
                     ADGTVDVVIGTHRLLSEDVHFKRLGLLVVDEEQRFGVSHKEKIKELKTDVDVLTLTAT
                     PIPRTLEMSLTGIRDLTLLNTPPAERQPILTYVGEYDDRAVGEAIRRELLREGQVFFV
                     HNRVHDIEHTAAHIRELVPEARVAVAHGQMDEGSLEKVVLDFWENEYDVLVC"
BASE COUNT          447 a          933 c          978 g          445 t
ORIGIN      
        1 gcagacgagc acgtcgtact cgttctccca gaagtcgagg acgaccttct cgagtgaacc
       61 ctcgtccatc tggccgtgcg cgacagcgac gcgagcctcg ggcacgagct cgcggatgtg
      121 cgccgcggtg tgctcgatgt cgtgcacgcg gttgtgcacg aagaagacct gcccttcgcg
      181 aagcagctcg cgacggatgg cttcgccgac cgctctgtcg tcgtactcac ccacgtacgt
      241 gaggatcggt tgacgctccg ccggtggtgt gttgagcaac gtgagatcgc ggatgccggt
      301 gaggctcatc tcgagtgtgc gcgggatggg cgtcgccgtg agcgtcaaga cgtcgacgtc
      361 ggtcttgagc tccttgatct tttccttgtg gctcacgccg aagcgctgct cttcgtcgac
      421 gaccagcaag ccgagtcgct tgaagtgcac gtcctcggac agcaaccgat gtgtcccgat
      481 gacgacgtcg accgtgccgt cggcgagacc gtccacgacc ttcttcgcct ggcccggcgt
      541 gaggaaccgc gacagcacct cgacccgcac ggggtagccg gcgaagcgct cggtgaacgt
      601 ctggctgtgc tgttgcgcga gcaaggtcgt cggcacgagg actgctgcct gcttgccgtc
      661 ctgcaccgcc ttgaagaccc cccggatggc cacctccgtc ttgccgaatc cgacgtcgcc
      721 acagacgaga cggtccatcg gcatcgaccc ctccatgtcg gccttcacgt cgacgatggc
      781 cttcagttgg tcgggcgtct cctggtaggg gaacgcatcc tcgagctcct gttgccacgg
      841 cgtgtcgggc gggaaggcgt ggccgggcgt gttcaaccgc ttctggtaga gcacgacgag
      901 ctcttgcgcg atctgcgtga cctcggctcg gacacgggcc ttggtcttcg cccagtcgcc
      961 gccattgagg cgactcaagg acggctgatc gccgccggtg tagtggcgaa cgctgtcgat
     1021 ctggtccgac gggacgtaca acttgtcgtc gccgcggtac tcgagcagca ggtagtcacg
     1081 ctcgaccccc gagatcgacc gtttcaccat gccggcgaag cgcgccacac cgtgctgatg
     1141 gtgcacgacg tagtcgcctg ggttgaggtc gtcgaagaag cgctcggcgt ctgtgcggcg
     1201 acgggcgcgg acgggacggt gcgcgcgccg gcgcccggtc agatccgggt ccgccagcaa
     1261 cgcgagcttg atggcgggaa ggatgaatcc gcgctcgagc ggcgcaaccg cgacttcggc
     1321 cttcagcccg tgctcgtcga ggagacggag gagccgcgcc ttcgagcctt caccgtcggc
     1381 actcacgacg acccggtagc cctgcgcgct gaggttcgcg agctgcgaca tcaagcgctc
     1441 gccgccgccg atgaccgggt cccatcccat ggcctggatc gtggtgacgt ccggaccctc
     1501 gggcgcgacc gtgacggtcc acgccgaggc gttcgtgtgc acgagcaacc ggtcgaaggg
     1561 caagtgcagg ctcgggaact cgcccaccgt gtcggcgccc catgtcttcg ccagcgaccg
     1621 agcgagatcg gcttcctcag cggcgatgtc cgcggcccga tcgcgaaggc gcttcggctc
     1681 gacgagcacg atctgcgcgt cggcgggaac gagatcgaag agcacgtgct cggtgtcggc
     1741 gagccacggc agccatgact ccatgccgtc gaaggtcaag ccatcggaaa gccgctccca
     1801 ctgctcgcgg ccccacggtt gcagcgacac gagccgtccg gcccgagcac gcacctcgtc
     1861 actgggcagc agctcgcggc acgggaagat ctgtgcctcg gtcaggtcgg cgatcgagcg
     1921 ctggtcgttg accgagaact ccgccagcct gtcgacctcg tcgccccaca agtcgatgcg
     1981 gacggggtgg tcagctgtgg aggggaacac atcgacgatc gacccgcgga cgccgacttc
     2041 tccccggtgc tcgacttgct gctcgcggcg gtaccccgcg ttcacgaggg cagcgacgag
     2101 ctcgtcacgg tcgcgccgct cgccgggccg cacctcgatg ggttcgacct tgtcgatgtc
     2161 cggaccgagt cgctgcacga gtgcccggat cggagcgacg atcacgtccg gtgtgcgggc
     2221 ggggtcgagc agacgccaga tcgtgcggag gcgccggccc atggtctcca cggcgggact
     2281 gacacgctcg aacggcagcg tctcccacgc ggggaacagg tcgacccggt ccgagcccag
     2341 gtaggcggcg aggtcgtggg ccagccgttc ggcgtctgcc gtcgtcggca cggcgatgac
     2401 gtacggacgc cgctgcgtcg tggcgacgag gccggcgagc gtgaaggcac gtgccggctc
     2461 gggcacggcg atgagcgcgg acgaccgccc ccgaacttcg agaagggcgg ggtcatcgcg
     2521 aagcagcgtg ggaagttcac gaacgctcat cagcgaacgc ccaacgctac cggccacagt
     2581 cgcgaactcc ggggagaaac gggcgctatg acacccgttt ccacccaaca ttcgggtgaa
     2641 ggacggtcac gggcggaccc agagggtcca gcgggtgagg gcggtgtagc cgaggcgttc
     2701 gtagacggga cggccgaggt cgctggcgat gaggacagcc ggcagcgtcg ggtccgccgt
     2761 cgtcgccgcc cacgtgacgg cggcgccgta gccacggccg cgt
//
LOCUS       DATBFZ010000452        24995 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_1957992, whole genome shotgun sequence.
ACCESSION   DATBFZ010000452 DATBFZ010000000
VERSION     DATBFZ010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 24995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24995
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1957992"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1101
                     /locus_tag="VH419_13725"
     CDS             <1..1101
                     /locus_tag="VH419_13725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017933806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY7044723.1"
                     /translation="RFWHTWPMDAPQRRSRAARLKKPPSLRRDRWLATDEPRGVWGRM
                     LENVAVLVYDGVAPFELGVLCEAWGLDREDQGVPNFDFAICAPQPGRIPTSLPGFALE
                     VSEGLERLADADLICLPAMPRHDPVPEDVVAALRDAHERGAKLMSVCAGAFVLGEAGL
                     LDDRLCTTHWRYVDELEARYPQAKVRCDVLYVDDGPIITSAGSAAGLDACLHLIRSEF
                     GASVAATVARRMVVAPHRDGGQAQFIESPVPETTADTLQPLLEWVSANLGEDLSVQTL
                     AAKAMMSPRTFARRFKAETGTTPYHWITNQRVLLAERLLEESDETIERIATRVGFSNA
                     TAFRHHFARLRGTSPQRYRRIFRTPSDQFAAS"
     gene            1151..1846
                     /gene="coaE"
                     /locus_tag="VH419_13730"
     CDS             1151..1846
                     /gene="coaE"
                     /locus_tag="VH419_13730"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012920727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEY7044724.1"
                     /translation="MSTATFPQFAVAQQARTLVCNAGGMLRVGLTGGIGAGKSAVSQM
                     LADHGAVVIDSDVLAREVVAAGTPGLAEIVIAFGPDMLDDNGELDRAAMGRRVFEDEE
                     ARQRLEKIVHPRVRARAAEIEQAAGADAIVVHDIPLLVETGQADTFDVVIVVDASTDV
                     QVQRLAEHRGMSEDDVHARIAAQATRDERLAAADLVLSNNGSWEQLRTAVDQAWEDLS
                     AQAARSGSASRRS"
     gene            complement(1807..2703)
                     /locus_tag="VH419_13735"
     CDS             complement(1807..2703)
                     /locus_tag="VH419_13735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007077946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY7044725.1"
                     /translation="MYLDEIARTPLLDAATEVELSKAIEAGLLAKHLLETGRIARAKG
                     GAPKRATREELEWLVQEGERATQRFIQANLRLVVSIARKYGRSAMPMLDLVQEGNTGL
                     IRAVEKFDYAKGYKFSTYATWWVRQAITRGIAQQARVVRLPVHVVEELNQIGSARRTL
                     ERQLGREPEPVEIAAELDMEVERVVDLINWGRDHVSLDTPVDEDGDTSLGDLVARETT
                     PGPDSAVLDVESRQRLDDLVGLLDPRSADVIRSRYGLTDGRQEKLADIGARHGVSAER
                     VRQIEREALAQLRRLADPDLAA"
     gene            complement(2990..5149)
                     /locus_tag="VH419_13740"
     CDS             complement(2990..5149)
                     /locus_tag="VH419_13740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020388253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEY7044726.1"
                     /translation="MTSKTAEAATDVPLPSAPKHPVERVHHGDTFVDHYEWLRDKEDS
                     DTRAYLEAENAYADAQMAHLADLREAIFGEIKTRTQETDLSVPSRTGGYWYYRRTQEG
                     QQYPIACRAKAIADDWTPPALEPGLDVPGEEVLIDCNELADGHNFFSLGAFSVSLDET
                     LLAYSTDVKGDERYTVRVKDLRTGELLPDEIPNTMYDVTWSADGSHLFYVTVNDAWRP
                     DKIWRHELATDAAADVVVYEETDERFFVSIGRTTSDRFLVVSAASKITSEVRLLEADQ
                     PTGEFRVVVPRADGIEYDIDHTVVGGRDHLLVLHNKDALNFTLGIGPATLASLDELDT
                     VIAHDDKVRLSDLTVSATTVAVNLREDSLAQVRVFSVGTDGLGQGTNIAFDEELFGAG
                     AVGFSDWNQPFVRLHYGSFVTPSSVYEYDPQTEELHLRKRQPVLGGYEPTDYVQTREW
                     VTARDGARIPIGIVRHKETSERGGSPTLLYGYGAYEASYDPAVSIPRLSLLDRGMVFV
                     LAHVRGGGEMGRTWYEQGRLLHKKNTFTDYIDVAKHLIDTGWTSTPQLAGIGGSAGGL
                     LMGAVTNMAPELFGGIVAHVPFVDALTSILDPSLPLTVIEWDEWGDPLHDPDVYAYMK
                     SYTPYENVAPRDYPAIYAHTSINDTRVLYVEPAKWVAKMRDTLPDSSRVLFKCEMSAG
                     HGGASGRYDAWREQAEWHAWVIDTVGASHTPLRALGD"
     gene            5850..6572
                     /locus_tag="VH419_13745"
     CDS             5850..6572
                     /locus_tag="VH419_13745"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002529034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HEY7044727.1"
                     /translation="MTGRYELNADVGEGFDTDAELLTIVSQANVACGFHAGDVATMRT
                     VCTLAMENGVAVGAQPSYRDRANFGRADMEMGYDDLARDVNEQVQTLQRIAEQVGATV
                     TYLKPHGALYNRIVRDEQQARAVVDTALAHGLPLMTLPGSTAERLMAEGGGMVIREFF
                     ADRGYLADGRLVPRSDEGALVTDPVQVGDRVRWLIASGTVVAVDGTPLEVRADSICVH
                     GDSPGAVALARAVRDALGSAVA"
     gene            6569..7201
                     /locus_tag="VH419_13750"
     CDS             6569..7201
                     /locus_tag="VH419_13750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012924099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allophanate hydrolase subunit 1"
                     /protein_id="HEY7044728.1"
                     /translation="MTVRLSRRPYGGHAVLVEVADGEAVAAAYAHIDRMVEAGRLERP
                     RDVVPAARTILVDGVDPDPWWADLVATGPVQPGQADVTHGRLVTLPIRYDGEDLDEVA
                     RQWSCEPEDVVRRHRESEFTVAFCGFAPGFAYCTSRPALPTVTRRDDPRTKVPAGAVG
                     LAGDYCGVYPKQMPGGWQIIGSTGEEMFDASRNDPALLHPGDRVRFEAED"
     gene            7204..8082
                     /locus_tag="VH419_13755"
     CDS             7204..8082
                     /locus_tag="VH419_13755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HEY7044729.1"
                     /translation="MGLLVHATGPLLLVQDRGRSGLMHLGVPPSGALDIEALALANRL
                     VGNPESAAGLEVLLGGVRLESQTSTRVALTGARAVLRIGGRAASWGVAESVRPGETIE
                     IEPAPDELRSWLAVAGGLDVPEVLGSRSTDTLTGLGPPTVAAGDRLAVGSAGGAPEED
                     AAGVPQGRPEVWSLPVEPGPRADWFTAASSERLVDQEYVVSASSNRTALRLDADTPLE
                     RSVDEELVSEGIVTGAVQVPASGRPLVFLADHPVTGGYPVVGVVTSTGLAACAQARPG
                     DRLRFQRALHGHHVVG"
     gene            complement(8098..9462)
                     /locus_tag="VH419_13760"
     CDS             complement(8098..9462)
                     /locus_tag="VH419_13760"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012921038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEY7044730.1"
                     /translation="MRNEASILHLDLDAFYAAVEQRDKPSLRGKPVVVGGVGLRGVVA
                     TASYEARRFGVRSAMSTAEARSRCPHAAFLVGRFDAYRASSVRVMRTLRALSPLVEPL
                     SLDEAYVDLAAAPRTLDLSPAGLEQVAAELKDQVRAVTGGLTASVGIGTSKLIAKIAS
                     DLDKPDGTVVVPPGSEPDLLGPMQVTVIPGVGPATAGRLHHVGVDTVEELRVLSVEEL
                     VRIVGQAHGTSLYALARAEDDRPVVADRETKSISVEDTFEVDLVDPVLLAAIIDRHAR
                     NVCARLARAKLSGRTVTVKVRLHDFTTLTRSATIAGPTDRPKVVSRLARKLLGELDTS
                     GGVRLLGVGVSGLADWIQDDLFVDEEEAADEPSTVDADAEIAEIAETRPRHGRGWMPG
                     MDVEHARYGPGWVWGSGLGRVTVRFETADTGAGAVRTLAADDADLVRRPVPTEELEDD
                     LEAG"
     gene            9543..10505
                     /locus_tag="VH419_13765"
     CDS             9543..10505
                     /locus_tag="VH419_13765"
                     /inference="COORDINATES: protein motif:HMM:NF025200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4192 domain-containing protein"
                     /protein_id="HEY7044731.1"
                     /translation="MSNPVPVTKVRNPADVVAIVPYLLGFQPTDSIVVVSLQGSRNRL
                     GPCARLDLVDELGDVPHEVDYLAGIVEQHRFRRVVLVAYSERVDLVEVLMHELIGRLS
                     ADGVTVVEAVRADGTRWWSYVCDDHTCCPSDGTAYDPSTSRVAVEAVAAGLSRASSRD
                     ALRSVFDPRPDLQARLVGALSRSPECDVLAVDEAVGRVRSLLGHRDHLSMQDRADLVR
                     AVQDLAVREAVWSMMTRADAEDHFALWLDVLQATPDDLMAPVGGLAAFAAWLTGRGVL
                     ASHGAERVLAVHPGYSMAVLLLELCAASVNPAVWTKLSSGRAVG"
     gene            10674..12152
                     /locus_tag="VH419_13770"
     CDS             10674..12152
                     /locus_tag="VH419_13770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012921040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEY7044732.1"
                     /translation="MGQEVERREFSREDRRRYREKVRQCLDVFARMLRESRFEFERPK
                     SGLEVELNLVDEKCDPAMKNAEVLEAIADPDFVTELGQFNLEINVAPRRLSGGGVNAF
                     EQSVRTSLNAAESKARSVGAHMVMVGILPTLQTSHMSTDTLSANPRYRLLNDQIFAAR
                     GEDMDISIRGVDRLRVTADSIMPEAACTSMQLHLQTSPDDFPKYWNAAQAVAGVQLAL
                     GANSPFLFGKELWAETRIALFEQATDTRSEELKSQGVRPRVWFGERWINSIFDLFEEN
                     VRYFPALLPICDDEDPEEVLDKGDTPGLHELRLHNGTIYRWNRPVYDVVRGVPHLRVE
                     NRVLPSGPSVVDMMANAAFYFGLIRSLVADERPLWSQMSFSAAEENFHAGARDGMAAQ
                     VYWPGLGTIGAPELVLRRLLPRARDGLDRWGIDADERDRLLDIIEQRCLTGRNGSTWQ
                     VQMFHHLFDSGATDRFDALREVVRRYSEYMHQNLPVHEWPVE"
     gene            12278..12682
                     /locus_tag="VH419_13775"
     CDS             12278..12682
                     /locus_tag="VH419_13775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY7044733.1"
                     /translation="MGVIVVGYVPKAEGRAALRLAAEEAKRRDASLVVVNSHRGGREF
                     DSDDAVESEAQLDDVRNQLKDAGVQHEVRQLVRGMDPAEDLIKVAEEVSAEFIVIGLR
                     RRSPVGKLILGSNAQRILLDAPCPVLAVKAAE"
     gene            12679..13251
                     /locus_tag="VH419_13780"
     CDS             12679..13251
                     /locus_tag="VH419_13780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008361828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HhH-GPD-type base excision DNA repair protein"
                     /protein_id="HEY7044734.1"
                     /translation="MSTLQITQDEAADEVLSTNAFALLAGMLLDQQFPMERAFAGPAK
                     ILDRFGTLDPDTIASAPPEEFAAVCATPPAVHRFPGAMAARIQELARHVVGEYDGRAA
                     SLWESAPTGAELLRRVQALPGFGKQKAQIFVALLGKQLDVRPAGWEQAAGVYAEEGSY
                     RSVADVRDAASLDKVRSFKKAQKAAAKAGG"
     gene            complement(13266..13961)
                     /locus_tag="VH419_13785"
     CDS             complement(13266..13961)
                     /locus_tag="VH419_13785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017934166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEY7044735.1"
                     /translation="MTLDSVLDLRLSPTRRTEVHRHRERAATQREQLFAVLRDALVCH
                     VGVILGGHPAVLPLAFGVDADGPDEGGTLYLHGSVAAGALSAAPDSDICVTVTHVDGL
                     VLARSAFHHSMNYRSAVVVGRARRVEDDHEKRHALDLIVDHVVPGRSGTLRPHTRKEL
                     AATAVIALPLHEASVKIRTGGVSDDPEDVEAGGVWAGVLPLRVVADNVVVDETAAALD
                     VPADVLRRRAQLA"
     gene            14052..15443
                     /locus_tag="VH419_13790"
     CDS             14052..15443
                     /locus_tag="VH419_13790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012917737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEY7044736.1"
                     /translation="MTTATPLAVHLDRSGDQALPVQLARQIRALVADGSIRPGQRLPS
                     SRALARELQVARAVVEAAFDQLLAEGWVVSRQGAGTFVQDVGSRARRGDVARTTPDAV
                     AAEGHELISFDTGTPYVDPRLKSGWRRAWREMSMAEPPRGYPEPTGIPELRELLATYV
                     GHRRGVTCSPEEVLVTAGTTHGLGLVLSTLRPGAVAVEDPGYRAAVEISQASGFDVVD
                     VPVDDEGLDVRTLAVSRRRDIRAVYVTPAHQHPTGVTLSASRRLALVAEARRRGSLIV
                     EDDYDSEFRYDVAPLPALASLSREVVYLGTAAKSVHPALRIGWLVGPAELVAAIAQQR
                     AVRRDHPSWPVQRAFLAMLRDGHVDRLIRSARRVYAARSEAVSRRLAAYGPVQGSGAG
                     MYVTVTLAEEAARRVSAAARKAGFDVPVLSLYARTHRRHGLVLGFGGVTDDELDRCLD
                     VVESTLSRTQAMA"
     gene            15535..17253
                     /locus_tag="VH419_13795"
     CDS             15535..17253
                     /locus_tag="VH419_13795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020577620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY7044737.1"
                     /translation="MSSSTSRTLPESLLSHPSVMRLIARGQHHGQVSANDIRDTCDEA
                     DITAAQRKALLKHLAGHGVSVAVGAEDAAGRRKTVAAVAKRATVSTATKKTAAKKAPA
                     KKATTTKSAAKPASRSTTKSGDGTARKPAAKKAATVTKTSSRSTPVKQTSAKTEAKPV
                     AKKTTAKATAKKAAATKKAPATSAANKPAGTPVEKDSVERVEPMVEIEAAAILLDADV
                     DGDKKTLADIDDSVFEKDLENDPTLKEDEKEGFTLSEADEADEPEQTVTVAGATADPV
                     KDYLKQIGKVPLLNAEMEVELAKRIEAGLFSEEKLGREMKLSAKMLDELGWIAEDGRR
                     AKNHLLEANLRLVVSLAKRYTGRGMLFLDLIQEGNLGLIRAVEKFDYTKGYKFSTYAT
                     WWIRQAITRAMADQARTIRIPVHMVEVINKLARVQRQMLQDLGREPTPEELAKELDMT
                     PEKVVEVQKYGREPISLHTPLGEDGDSEFGDLIEDSEAIVPADAVSFTLLQEQLHAVL
                     DTLSEREAGVVSMRFGLTDGQPKTLDEIGKVYGVTRERIRQIESKTMSKLRHPSRSQV
                     LRDYLD"
     gene            17317..17844
                     /locus_tag="VH419_13800"
     CDS             17317..17844
                     /locus_tag="VH419_13800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEY7044738.1"
                     /translation="MRDTNRWTRCAHGHRHWGSLGAAGLMLTRRTPDSPAGTPEVLLQ
                     HRADWVHHGGTWSVPGGAIQRDETAWETALREVREETGLDVGAAHELARYVDDHGGWS
                     YTTIIATLDTNTGVSATLAQIGWLDTILIGEQQDLSWRPVAAVDGLALHPGFVDSWPA
                     VRSLVLTTPTPAPRE"
     gene            complement(17816..18205)
                     /locus_tag="VH419_13805"
     CDS             complement(17816..18205)
                     /locus_tag="VH419_13805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017933743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY7044739.1"
                     /translation="MTLRWQCVCVNTTDPGRLAPFWEQTLGWRRIYDAPDEVALGPPA
                     DSPEDGVSPELLFLQVPEEKSVINRLHIDLRPDDQAAEVERILALGATRADVGQGDDV
                     SWVVLADPDGNEFCVLRALTPEEQASG"
     gene            18301..18639
                     /locus_tag="VH419_13810"
     CDS             18301..18639
                     /locus_tag="VH419_13810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020579185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY7044740.1"
                     /translation="MASSYIKFVTVDCDDPIKVATFWAAALGSNLDEDATDERAFVEA
                     PGWGGPNMWFVKVPEPKSAKNRIHFDLRAPGTISDEVDRLTALGATVQHVRDDLVVMQ
                     DPGGNEFCVE"
     gene            complement(18652..19137)
                     /locus_tag="VH419_13815"
     CDS             complement(18652..19137)
                     /locus_tag="VH419_13815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HEY7044741.1"
                     /translation="MERTAVTFLVGVAIVVGLVGVVVPVLPGQLLVLGAIGVWAFAEA
                     SPTGWAVFAVALVSVGVSQVVKYVVPSKRLLAAGVPARSLTVGGLVGIVGFFVVPVVG
                     LFIGFPLGVYVAEHVRLRSPQAAWTSTRQAITAVGLSMLIELAGSLVAAGTWLAAVIA
                     A"
     gene            complement(19301..19558)
                     /locus_tag="VH419_13820"
     CDS             complement(19301..19558)
                     /locus_tag="VH419_13820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044742.1"
                     /translation="MSSEEGKAPVTTAVAPSPQITALDRCDRCGAQAYVRVELVSGGE
                     LLFCAHHAREHAEKLQLVAASIHDETAKLADTPSTAPDDER"
     gene            20052..22154
                     /locus_tag="VH419_13825"
     CDS             20052..22154
                     /locus_tag="VH419_13825"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020388289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEY7044743.1"
                     /translation="MPTRGHVGGAVDVVDASYTARHLLVLEGLEAVRKRPGMYIGSTD
                     TRGLMQCVWEIIDNGVDEALAGVCSHITVSLYADGSVEVHDDGRGIPVDVEPKTGLSG
                     VEVVFTKLHAGGKFGGGSYVATGGLHGVGASVVNALSARLDVVVDRSPASWAMSFRRG
                     VPGTFDGEGPRAAFTRREGSITKVGKVKKGVTGTRIRFWPDRQVFTKDAAYSYDELVT
                     RARQTSFIVPGLAISIRDERAAEVVEETFRHDGGITEFCEFLATDEKVTDVLRLHGVD
                     NFTETVPVLDDAGHMTPQDVERELTVDVALRWGSGYDTEIRSFVNVIATPKGGTHVTG
                     FERALTKTFNDVVRATRGMLKAGESDLVKDDILEGLTAVVTVRLAEPQFEGQTKDVLG
                     TPAASRIVAKIVSAQVRAFLTSTKKHQKPQARALLEKVVNAARTRVAARQQRDTQRRK
                     SALESSALPAKLADCRSNDVDRSELFIVEGDSALGTAKLARDSEYQALLPIRGKILNT
                     QKKSAGEMLQNAECAAIIQVVGAGSGRTFDLEQARYGKVIFMADADTDGAHIRCLLAT
                     LFHRHMHGLLAAGRVYAAVPPLHRIELSNPKKGMDRYVYTYSDDELQRKLAELSKKNV
                     RWKDPVQRYKGLGEMDAHQLAETTMDPRHRTLRRITIDDAGSAEQMFELLMGNDVGPR
                     REFIVQGAYELDLEAIDA"
     gene            22326..23627
                     /locus_tag="VH419_13830"
     CDS             22326..23627
                     /locus_tag="VH419_13830"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY7044744.1"
                     /translation="MATATRSWRGGGRHRRHRSLALIVVLAALSALLAACGGDSGKTT
                     LYWYINPDVGGQDAVAKNCSTDQYTISTEVLPQNATDQRVQLARRLAAHDSAIDLISL
                     DPPYTAEFSNAGFLAAIPKDEQQRLADQSFGGATKAATWDGQIVAFPFWSNTQVLWYR
                     KSFVEKAGIDMSKPVTWDELIDVASKNGGQLGVQANKYEGYVVWINALYAGAGGQLVS
                     NTERGADLTIDIDSPAGDKAAAVIQKLSSSPAAPPDLSVSNEGTAGSTFGGPAGSFMV
                     NWTYIFHNYDATDPDVAKDIGYTRYPETVAGEQSGPPYGGIGIVVSEYSSHKDFALQA
                     AACLVSPKNQGINAEITGNMPASAAGYQYPPLQKLYPADLLALFQQSVETSAPRPSTP
                     YWSDISGSIQSTWHPPDSVNQDTPKKSAAFTEQVINGERLL"
     gene            23624..24574
                     /locus_tag="VH419_13835"
     CDS             23624..24574
                     /locus_tag="VH419_13835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017936704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEY7044745.1"
                     /translation="MTTTTTAAPPTKRATKKPTSDRSVAENRLGRRLVAPAVIMMLIV
                     TAWPMIKALYDSTYRYRLTAPDDKEFVGLGNYATVLTDSLFWRDTWNTVIIMVVTVAV
                     ELVIGFAFAMVMHRIIFARGVMRTAILIPYGIVTVVSAFAWQFAFSINNGFVNDWFSW
                     LPWISPDTNWFGSHWPAMFAIMISEIWKTTPFMALLLLAGLSQISEDMLEAAKVDGAT
                     WWQRLYKVILPNMRAAIMVAVLFRALDAFRIFDNIYIMTKGAQNTESSSFLTYRQVIE
                     QMQLGLGSALSVLLFLTVLLLAGIIVKLFKVDLAQARGQS"
     gene            24571..>24995
                     /locus_tag="VH419_13840"
     CDS             24571..>24995
                     /locus_tag="VH419_13840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017936703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEY7044746.1"
                     /translation="MRNKSGIGVWVGFLAIMIWCLLPVAWIISLSFKSVAETTVGSPH
                     FLPDDPTWQNYKDILQNDDFQLALRNSFGIAFISTALSVVLATLAAYAIARLEFKGKR
                     FVLSTALAIAMFPVVALVGPLFDMWRTFGLFNTWLGLIIP"
BASE COUNT         4080 a         8397 c         8562 g         3956 t
ORIGIN      
        1 agattctggc atacttggcc gatggacgcg ccgcagcggc gtagccgcgc ggctcggctc
       61 aagaagccac cttccctccg acgggatcga tggttggcga ccgacgaacc acgaggagtg
      121 tggggccgga tgctcgagaa cgtggcggtg ctggtgtacg acggggtcgc gccgttcgag
      181 ctcggcgtcc tgtgcgaagc atggggcctg gaccgggagg accagggcgt tcccaatttc
      241 gacttcgcga tctgtgcgcc ccagcctggt cggattccga ccagtctgcc cgggttcgcg
      301 ttggaggtct ccgagggtct cgagcggttg gccgacgcgg acctgatctg cctgcccgcg
      361 atgccgcggc acgaccccgt cccggaagac gtggtggctg cgctgcggga cgcccatgag
      421 cgtggcgcga agctgatgtc ggtgtgcgcc ggcgcgttcg tgctgggaga ggcggggctg
      481 ctcgacgacc ggctgtgcac aacgcactgg cgctacgtcg acgagctgga ggcgcggtat
      541 ccacaggcca aggtgcgctg cgacgtgctg tacgtcgacg acgggccgat catcacctct
      601 gccggttcgg ccgccgggct ggacgcctgc ctgcacctca tccgcagcga gttcggcgcc
      661 agcgtcgcgg cgaccgtggc gcgacggatg gtcgttgccc cgcaccgcga cggcggccag
      721 gcgcagttca tcgagtcgcc ggtgccggag acgactgctg acaccttgca gccgctgttg
      781 gagtgggtca gcgccaacct gggcgaagac ctgagtgtcc agacgttggc ggccaaggcg
      841 atgatgtcgc cgcgaacgtt cgcccgtcgc ttcaaggcgg agaccggcac gacgccgtac
      901 cactggatca ccaaccagcg ggtcctgctg gccgagcggc tgctcgagga gtccgacgag
      961 accatcgagc ggatcgcgac ccgcgtcggc ttcagcaacg ccaccgcctt ccgccaccac
     1021 ttcgcccggt tgcgcggcac gagcccgcag cggtaccgac ggatattccg taccccgtcc
     1081 gaccagttcg ccgccagctg agcactcctg gggtcccgcg aattgcggga aacctcatcg
     1141 tcgtacggac gtgtcgacgg caactttccc gcaattcgcg gtcgcacagc aggcgcggac
     1201 gttggtgtgc aacgctggcg ggatgttgcg ggtcgggctc acgggcggaa tcggcgcggg
     1261 gaagtccgcc gtcagtcaga tgctcgccga ccatggtgcc gtcgtcatcg actccgacgt
     1321 gctcgcgcgc gaggtcgtcg cggccggtac gccgggactg gcagagatcg tcatcgcgtt
     1381 cggcccggac atgctcgacg acaatggcga gctcgaccgt gccgcgatgg gtcgccgcgt
     1441 cttcgaagac gaggaggcgc ggcagcggct cgagaagatc gtccatcccc gagtacgagc
     1501 ccgcgccgcc gagatcgaac aggcagcagg agctgatgcg atcgtcgtgc acgacatccc
     1561 gttgctcgtc gagacgggtc aggccgacac gttcgacgtc gtcatcgtgg tcgacgcgtc
     1621 caccgatgtg caggtgcagc gcctggccga gcaccgcggg atgtcggagg acgacgtaca
     1681 cgcgcggatc gccgcccaag cgacccgcga cgagcggctc gcagctgccg acctcgtcct
     1741 cagcaacaac gggtcatggg agcagctgcg aaccgccgtg gatcaggcct gggaggacct
     1801 gtccgctcag gccgccagat cagggtccgc gagccggcgc agctgagcga gtgcctcccg
     1861 ctcgatctgc cggacgcgct cggccgacac accgtgccgg gcgccgatgt cggccagctt
     1921 ctcctggcgg ccgtcggtga ggccgtaacg ggaccggatc acgtcggccg agcgcgggtc
     1981 gagcaggccg accaggtcgt ccagacgctg tcgggactcg acgtccagga cggcggagtc
     2041 cggaccgggc gtggtctccc gagccaccag gtcgcccagt gacgtgtcgc cgtcctcgtc
     2101 gacgggcgtg tcgaggctca cgtggtcgcg gccccagttg atgaggtcga ccactcgctc
     2161 gacctccatg tcgagctcgg cggcgatctc caccggctcc ggctcacgac ccagctgccg
     2221 ctccagggtg cgccgtgccg aaccgatctg gttgagctct tccacgacgt gcacaggaag
     2281 gcggacgacc cgcgcctgct gcgcgatgcc gcgggtgatc gcttgccgaa cccaccaggt
     2341 ggcgtacgtg gagaacttgt atcccttcgc gtagtcgaac ttctcgaccg cgcggatcag
     2401 gccggtgttg ccttcctgca ccaggtcgag catcggcatc gccgagcggc cgtacttgcg
     2461 tgcgatcgag acgaccagcc gcaggttcgc ctggatgaag cgttgggttg cccgctcgcc
     2521 ttcctggacg agccactcca gctcctcacg ggtcgcccgc ttcggggcgc cacccttcgc
     2581 ccgcgcgatc cggccggtct cgagcaggtg cttggccagc aggccggcct cgatcgcctt
     2641 cgaaagctcc acctcggtgg cggcatcgag cagcggcgta cgggcgatct cgtcgagata
     2701 catgcccaca ctgtcgcggc cttcgatcgc gccatcgcgc gcgtcgagcg tcgtggtacg
     2761 acccgtcaat gagcgactct gtgccacggc cttcctctcc ttcggtggtg cgcaccagat
     2821 ggtccgtaag ggaacaacgc tccgggccca cccaagattc ctacttcgga gtaggttgtt
     2881 gcgaccgttg ccggtcctac ctggggaaac gccaccgtca ccgattcggt tgcccgtctc
     2941 agggagttct caagctttca gcgcgccacc ggcatcaccg aggtgaccgt cagtcaccga
     3001 gcgcgcgcag cggggtgtgg gaggccccga ccgtgtcgat gacccaggca tgccattccg
     3061 cctgctcacg ccaggcgtcg taccggccgc tggccccgcc gtgcccggcc gacatctcac
     3121 acttgaacag cactcggctg gagtccggca gcgtgtcccg catcttcgcc acccacttgg
     3181 ccggttcgac gtacaggact cgggtgtcgt tgatcgaggt gtgagcgtag atggccgggt
     3241 agtcccgcgg cgcgacgttc tcgtacggcg tgtaggactt catgtacgcg tagacgtccg
     3301 ggtcgtgaag cggatcgccc cattcgtccc attcgatcac cgtcagggga agggacggat
     3361 cgaggatcga cgtcagcgcg tcgacgaacg gcacatgggc gacgatgcca ccgaacagct
     3421 cgggcgccat gttggtgacc gcgcccatca gcaggccgcc ggcgctgccg ccgatcccgg
     3481 cgagctgggg cgtggaggtc caaccggtgt cgatcaggtg cttggcgacg tcgatgtagt
     3541 cggtgaaggt gttcttcttg tggagcagcc tgccctgctc gtaccacgta cggcccatct
     3601 cgccgccccc ccgcacgtgc gccaagacga agaccatgcc gcggtcgagc agcgacagcc
     3661 gggggatgct cacggccggg tcataactgg cctcgtaggc gccgtacccg tagaggagcg
     3721 tcggtgagcc gccgcgctca gaggtctcct tgtgccggac gatcccgatc gggatcctgg
     3781 cgccgtcccg tgcggtcacc cattcgcggg tctgaacgta gtcggtgggt tcgtagccgc
     3841 cgagcaccgg ttgccgcttg cgcaggtgca gctcctccgt ctgcgggtcg tattcgtaga
     3901 ccgagcttgg agtcacgaat gagccgtagt gcagccggac gaacggctgg ttccagtcgc
     3961 tgaagccgac cgccccggcg ccgaacagct cctcgtcgaa cgcgatgttg gtgccctgcc
     4021 cgaggccgtc ggtgccgacc gagaagaccc ggacctgcgc caggctgtcc tcccggaggt
     4081 tgaccgcgac tgttgtcgcg gacacggtga ggtcgctgag ccggaccttg tcgtcgtgcg
     4141 cgatgacggt gtcgagctcg tccagcgagg ccagcgtcgc cggaccgatg cccagcgtga
     4201 agttcaaagc gtccttgttg tgcagcacga gcaggtggtc tcgaccaccg acgacggtgt
     4261 ggtcgatgtc gtactcgatc ccgtcggcac gcggtacgac gacgcggaac tcacccgtcg
     4321 gctggtccgc ctccaacagc cggacctcgg acgtgatctt cgacgccgcc gacaccacga
     4381 ggaacctgtc gctggtggtc cggccgatcg agacgaagaa ccgctcgtcg gtctcctcgt
     4441 agacgacaac gtcggccgcg gcgtcggtcg ccagctcgtg ccgccagatc ttgtcaggcc
     4501 gccaggcgtc gttcacggtg acgtagaaga ggtgggagcc gtccgctgac caggtgacgt
     4561 cgtacatggt gttcgggatc tcgtcgggca gcagctcacc ggtacgcagg tccttgaccc
     4621 gcacggtgta gcgctcgtcg cccttgacgt ccgtggagta cgccaacagg gtctcgtcga
     4681 gggacaccga gaacgctccg agcgagaaga agttgtgtcc gtcggcgagt tcgttgcagt
     4741 cgatgagcac ttcttcgccg ggcacgtcca ggccgggctc gagcgccggc ggtgtccagt
     4801 cgtccgcgat ggccttggcg cgacaggcga tgggatactg ctgtccctcc tgtgtccggc
     4861 ggtagtacca gtagccgccg gtgcggctcg gcaccgagag gtcggtctct tgagtgcggg
     4921 tcttgatctc gccgaagatc gcctcacgca ggtcagcgag gtgcgccatc tgcgcgtcgg
     4981 cataggcgtt ctccgcctcg aggtaggcgc gggtgtcgga gtcttccttg tcgcgcagcc
     5041 actcgtagtg gtcgacgaac gtgtcgccgt gatgaacccg ctcgacaggg tgcttcggtg
     5101 cggagggcag gggaacgtcc gtggcggctt cggcggtctt cgaggtcacg tcgagaccct
     5161 actcggcgag acccgttgct gaccgagaat ttctcgtggc ccaggcgttg gtggctcgtg
     5221 tcaggcgtgc ccgtgcatcg agtcagacca cccggtcgca aggtacgaaa tcgccttcgc
     5281 ctgccgtttg tgtgagccgc gcctgctacc ggtgcattcc tcacgcgtca agggtcgctt
     5341 cgcgatcgct gacgcgaccg ccttcggcgt cccttgactc gtgagcctcg atgcaccggt
     5401 tcgggcaggc tccgacgggc aggactgccg tcctgcccgt cgcaccggcg cggcccccac
     5461 ctccggaaag gcacctacgc caccattcgg acatgtgtcg gtggcggttt gcctcggtag
     5521 ccatccaacc ggacgggtag ccaggcaggt ctgccttggg aggggtgagt ctgcctgggt
     5581 agccatgcaa accgcggctc ggcgaccgac ggatgtccga aaaccgccag tcacacacca
     5641 ggcaaaaggc acggaacgcc ggccgggcgc ctcaggaggc acgaacgtct ccgctcgtgc
     5701 tgccgacccg cctcagccga ggccgacgga ctgccggacg accacgcgtc gcccatcgcg
     5761 accgtccccg cccaaagaac actcgagaag tccccaggtc tgcctgggtg ccatgcaaac
     5821 cgccgccagc gtcgcgggag aatggccggg tgaccgggag gtacgagctc aacgccgatg
     5881 tcggcgaggg cttcgacacc gacgccgagc tgttgacgat cgtcagccag gcgaacgtcg
     5941 catgcggttt ccatgccggc gacgtcgcga cgatgcgtac ggtctgcacg ctcgccatgg
     6001 agaacggcgt tgccgtcggc gcccagccgt cgtaccgcga ccgtgcgaac ttcggacggg
     6061 ccgacatgga gatggggtac gacgacctgg cccgcgacgt gaacgagcag gtccagacgt
     6121 tgcagcggat cgccgagcag gtcggcgcga ccgtgaccta cctcaaaccg cacggtgccc
     6181 tctacaaccg gatcgtccgc gacgaacagc aggcgcgcgc cgtggtggac accgcgctcg
     6241 cgcacggcct gccgctgatg acgctgccgg ggtcaaccgc ggaacggctg atggccgaag
     6301 ggggtggcat ggtgatccgg gagttcttcg ccgatcgtgg ctacctcgcc gacggccggc
     6361 tggtaccacg gtcggacgag ggggcgctgg tgaccgatcc ggtacaggtc ggtgaccggg
     6421 tccgctggct gatcgcgtct gggacagtcg tggcggtcga tggcactccg ctcgaggtgc
     6481 gggcggactc gatctgtgtg cacggcgaca gcccgggcgc ggtcgcgctg gctcgtgccg
     6541 tgcgtgacgc gctcgggtcc gcggtcgcat gacggtccgc ctctcccgcc gcccgtacgg
     6601 cggacatgcc gtgctcgtcg aggtcgcgga cggcgaggcg gtcgccgcgg cgtacgcgca
     6661 catcgaccgg atggtcgagg cgggacgctt ggaacggccg cgcgacgtgg tgccggccgc
     6721 ccggaccatc ctcgtcgacg gcgtggatcc ggacccgtgg tgggccgacc tggtggcgac
     6781 cggtccggtc cagccggggc aagccgatgt cacccatggc cgtctcgtga cccttccgat
     6841 ccggtacgac ggagaggacc tggacgaggt ggcgcgccag tggtcgtgcg aacccgagga
     6901 cgtcgtacgt cggcaccgcg agtcagagtt cacggtggcg ttctgcggct tcgcgccggg
     6961 gttcgcctac tgcacgagcc gacctgcgtt gccgacggtg acccgacgcg acgacccgcg
     7021 cacgaaggtt ccggctggtg cggtcggcct cgcgggtgac tactgcgggg tctacccgaa
     7081 acagatgccg ggtgggtggc agatcatcgg cagcacgggc gaggagatgt tcgacgcgtc
     7141 gcgcaacgac ccggcgctgc tgcatccggg cgaccgggtt cgtttcgagg ccgaggactg
     7201 acggtgggtc ttctcgtgca cgcgaccggt ccgctcctgc tggtgcagga ccgtggccgg
     7261 tccggcctga tgcacctcgg cgtcccaccg tccggggcgt tggacatcga agcgttggcg
     7321 ctggccaacc ggctggtcgg caacccggag tccgcggcag gcttggaggt cctcctcggc
     7381 ggcgtgcggc tggagtcgca gacctcgacc cgggtggcac tcaccggggc cagggctgtc
     7441 ctgcggatcg gcggccgcgc ggcgtcgtgg ggggtggctg agtcggtccg gccgggggag
     7501 acgatcgaga tcgagccggc gccggacgag ctccggtcct ggctggctgt ggcgggcggt
     7561 ctcgacgtcc ccgaggttct cggaagtcgg tccacggaca cgctgaccgg cctgggcccg
     7621 ccgacggtgg cggcgggtga ccgactcgct gtggggtccg ccggtggcgc acccgaggag
     7681 gacgcggccg gggttccgca gggccgcccg gaggtgtgga gccttccggt cgaacccgga
     7741 ccgcgtgcgg actggttcac cgcggcttcg tccgaacgac tcgtcgacca ggagtacgtc
     7801 gtctcggcgt cctccaaccg aacggccctc cgcttggacg cggacacccc gctcgagcgg
     7861 tccgtggacg aagagctggt gagcgaggga atcgtgaccg gggcggtgca ggtgccggcg
     7921 tccggccgac ccctggtgtt cctcgccgac cacccggtga ccggggggta cccggttgtc
     7981 ggcgtcgtca cttcgacggg gctggcggcg tgtgcccagg cccggcccgg tgaccggctg
     8041 cgtttccagc gggcgctgca tggacaccac gtcgtgggct gaggacgttc gggtccgtca
     8101 gcctgcctcc aggtcgtcct ccagctcttc ggtcggcacc ggacggcgta cgaggtccgc
     8161 gtcgtcggcg gccagcgtgc gcaccgcacc cgcgccggtg tcggcggtct cgaaccgcac
     8221 ggtgacacga cccagccccg atccccatac ccagcccggg ccgtagcggg cgtgctcgac
     8281 gtccatgccc ggcatccagc cgcgtccgtg gcgtggccgg gtctccgcga tctccgcgat
     8341 ctccgcgtcg gcgtcgacgg tcgacggctc gtcggccgcc tcctcctcgt cgacgaagag
     8401 gtcgtcctgg atccagtcag ccagcccgga cacccccacc cccaacagcc gaacgccacc
     8461 agacgtgtcg agctcgccca gcagcttgcg tgcgagccgg ctcacgacct tcggtcgatc
     8521 cgtcggaccg gcgatggtcg ctgaccgggt gagcgtggtg aaatcgtgca gccgcacctt
     8581 gaccgtgacc gtccgaccgg acaacttcgc ccgggccaat cgtgcgcaga cgttgcgggc
     8641 gtgccggtcg atgatggccg cgaggaggac cgggtccacg aggtcgacct cgaaggtgtc
     8701 ctcgacggag atggacttcg tctcgcggtc cgcaacgacc ggccggtcgt cctcggccct
     8761 ggcgagcgcg tagaggctgg tgccgtgggc ctgcccgacg atcctgacca gctcctccac
     8821 gctgaggaca cgcagttcct cgaccgtgtc gacacccaca tggtggagcc gtccggccgt
     8881 ggccggtccg acgccgggaa tcacggtgac ctgcatcggc cccagcaggt ccggttcaga
     8941 ccctggtggg acgacgaccg tgccgtccgg cttgtcgagg tcgctggcga tcttcgcgat
     9001 cagcttcgac gtgccgatac ccaccgatgc ggtgaggccc ccggtcaccg cccgcacctg
     9061 gtccttcagc tcggcggcga cctgttcgag acctgctggg gagaggtcga gcgttcgcgg
     9121 tgcggccgcg aggtcgacat acgcctcgtc gagggacagt ggctcgacca gcggcgagag
     9181 ggcacgcaac gtccgcatga cgcgaacgct ggacgcgcgg taggcgtcga aacggccgac
     9241 gaggaaggcc gcatgcgggc agcgtgagcg agcctcggcg gtcgacatgg ccgatcggac
     9301 accgaaccgc ctggcttcgt acgacgctgt ggcgacgacc ccgcggaggc cgacgccgcc
     9361 gaccacgacc ggcttgccgc gcagcgacgg cttgtcgcgc tgctcgacgg cggcgtagaa
     9421 ggcgtccagg tcgagatgca ggatcgacgc ctcgttacgc acatgctcat catcgctgat
     9481 ggctcgtcca cagcttgctg ttcggcgtcc catcgccgag ccggtgctgc aacggtcgga
     9541 tgatgagcaa ccccgtccct gtcacgaagg tccgcaaccc cgccgacgtc gtcgcgatcg
     9601 tgccctacct tctcggcttc cagccgacgg acagcatcgt cgtggtctcg ctgcaaggtt
     9661 cccgcaaccg gctcgggccg tgcgcgcggc tcgacctcgt ggacgaactc ggtgacgttc
     9721 cacacgaggt cgactacctg gcggggatcg tcgagcagca ccgcttccgc cgcgtcgtgt
     9781 tggtcgcgta ctcggagcga gtcgacctcg tcgaggtgct gatgcacgag ctgatcgggc
     9841 ggctgtcggc cgacggggtg accgttgtcg aggccgtgcg cgccgatggc acccgctggt
     9901 ggtcgtacgt ctgtgacgac cacacctgct gtccgtcgga cggcacggcg tacgacccgt
     9961 cgacatctcg cgtcgccgtg gaagccgtgg cggcgggcct gtcgcgggcg tcgagccggg
    10021 acgcgttgcg gtcggtgttc gacccacggc cggatctcca ggcacgtctg gtgggcgcgc
    10081 tgtcgcgttc tccggagtgc gatgtgctgg ccgttgacga ggccgtcggg cgagtccgca
    10141 gcctgctcgg ccatcgagac cacctgtcca tgcaggatcg tgcggatctc gtgcgagcgg
    10201 tgcaggatct cgccgtccgg gaagccgtgt ggagcatgat gacgcgtgcc gatgccgagg
    10261 accacttcgc gttgtggctc gacgtcctcc aagccacacc ggacgacttg atggcgccgg
    10321 tcgggggact ggcggccttc gcggcctggc tcaccggccg cggagttctc gcctcccacg
    10381 gggccgagcg cgtcctcgcg gtgcatcccg gctactcgat ggcggtgctg ctgctcgagc
    10441 tgtgtgccgc cagcgtcaac cccgcggtct ggaccaagct ctcgagtgga cgagcggttg
    10501 gttgacgctc ttcatcgaca agctcacgac cgaaacccca gcccgaaacc cagcccgaaa
    10561 ctgttcgacg ggctccatct ggtagcggcg aggcgacaga aagggcaggc ttgcatcagc
    10621 gggcgcagga ccaccgagcc cgcgggcaga cgtcctgtga ggaggcaccg cggatggggc
    10681 aggaggtcga gcgccgagag ttctcgcgtg aggacaggcg gcgctaccgg gagaaggtcc
    10741 gacagtgcct cgacgtcttc gcgcggatgc tgcgcgagtc gcggttcgag ttcgagcgtc
    10801 ccaagtccgg gctcgaggtc gagctcaacc tcgtcgacga gaagtgcgat ccggccatga
    10861 agaacgccga ggtgctcgag gccatcgccg accccgactt cgtcaccgag ctcggtcagt
    10921 tcaacctcga gatcaatgtc gcgccgcgcc gcctttccgg cggtggtgtc aacgcgttcg
    10981 agcagagcgt acggaccagt ctgaacgccg ccgagtccaa ggcacggtcg gtcggcgccc
    11041 acatggtgat ggtgggaatc ctcccgaccc tccagacctc ccacatgagc acggacacgc
    11101 tctcggcgaa tccgcgctac cggctgctca acgaccagat cttcgccgct cgtggcgagg
    11161 acatggacat ctcgatccgt ggtgtcgacc ggctccgcgt gacggccgac tcgatcatgc
    11221 cggaagcggc gtgcacctcc atgcagctgc acctgcagac cagcccggac gactttccga
    11281 agtactggaa cgccgcacag gcggtcgccg gcgtccagct cgctctcggc gccaactcgc
    11341 cattcctctt cggcaaggag ctgtgggccg agacccggat cgccttgttc gaacaggcga
    11401 cagacacgcg gagcgaggag ctgaagtcac agggtgtgcg gccacgggtc tggttcgggg
    11461 agcgatggat caactcgatc ttcgacctgt tcgaggagaa cgtgcgctac ttcccggcgt
    11521 tgctgcccat ctgcgacgac gaggacccgg aagaggtcct cgacaagggt gacaccccgg
    11581 gcctgcacga gctgcggctg cacaacggca cgatctaccg atggaaccgt cccgtgtacg
    11641 acgtggtgcg gggcgtcccg catctgcgcg tggagaaccg cgtcctgcca tccgggccga
    11701 gcgtggtgga catgatggcc aatgctgcct tctacttcgg tctgatccgg tcgctggtcg
    11761 ccgacgaacg gccgctgtgg tcacagatgt cgttctcggc tgccgaggag aacttccacg
    11821 cgggcgcccg tgacggcatg gccgcgcagg tgtactggcc ggggctcggc acgatcggcg
    11881 ctcccgagct cgtgctccgg cggctgctgc cgcgggcgcg agacgggctg gaccgctggg
    11941 ggatcgacgc cgacgagcgc gaccggctcc tcgacatcat cgagcagcgt tgcctgaccg
    12001 gacgcaacgg gtcgacgtgg caagtgcaga tgtttcacca cctgttcgac tccggcgcca
    12061 ccgaccgttt cgacgcattg cgcgaggtcg ttcgccgcta cagcgagtac atgcaccaga
    12121 accttcccgt tcacgagtgg ccggtggagt gaccggggct cgctggggct ccatgggcgt
    12181 tgtcggcggc gtaccgctcc tcgatcgtct ccggtagcgt tcggcccacg gcaggactgc
    12241 gcacaaccag tcgacgttcg gtacgaagga gcgctccatg ggcgtcatcg tggtggggta
    12301 cgtgcccaag gcggaaggaa gggccgccct gcggctggcg gcggaggagg ccaagcgccg
    12361 cgacgccagc ctggtggtcg tcaactcaca ccgcggcgga cgtgagttcg acagcgacga
    12421 cgccgtcgag tccgaggccc agctggacga cgtgcggaac cagctgaagg atgccggtgt
    12481 ccagcacgag gtgcgccagc tggtccgcgg catggacccg gccgaggacc tgatcaaggt
    12541 cgccgaggag gtctcggcgg agttcatcgt gatcggcctg cggcgccgga gccccgtcgg
    12601 caagctcatc ctcggcagca acgcccaacg gatcctgctc gacgctccct gtccggtgct
    12661 cgccgtcaag gcagcggagt gagcacgctc cagatcaccc aggacgaggc cgccgacgag
    12721 gtcctctcga cgaacgcgtt tgcattgctg gccgggatgt tgctcgatca gcagttcccg
    12781 atggagcgcg cgttcgctgg tccggcgaag atcctggacc ggttcggcac gctcgatccg
    12841 gacacgatcg ccagcgctcc gccggaggag ttcgccgccg tgtgcgccac cccgcctgcg
    12901 gtgcatcgtt ttcccggtgc catggcggct cgtatccaag agcttgcgcg ccacgtcgtc
    12961 ggggagtacg acggccgggc cgcctcgctg tgggagagcg cgcccaccgg cgcggagctg
    13021 ttgcggcgag tacaggcact gccggggttc gggaagcaga aggcgcagat cttcgtcgcg
    13081 ctcctcggca agcagctcga cgtccggccc gccggctggg agcaggccgc gggcgtgtac
    13141 gccgaggagg gctcgtaccg ctcggtcgcc gacgtacgag acgccgcctc gttggacaag
    13201 gtgcgcagct tcaagaaggc gcagaaagcc gccgccaaag caggcggctg aggtcgaccc
    13261 gggagtcagg cgagctgggc gcggcggcgc agtacgtcgg cggggacgtc cagcgcggca
    13321 gcggtctcgt cgaccacgac gttgtctgcg acgacgcgca acggcaggac gccggcccat
    13381 actccgcctg cctcgacatc ctcagggtcg tccgacacgc cgcccgtccg gatcttcacc
    13441 gacgcctcgt gcagcgggag cgcgatgacc gcggtcgcgg ccagctcctt gcgcgtgtgc
    13501 ggccgcagtg tgccggagcg cccgggtacg acgtggtcca cgatcaggtc gagggcatgc
    13561 cgcttctcgt ggtcgtcctc gacgcggcgg gcccggccga ccacgacagc cgagcggtag
    13621 ttcatcgagt gatggaacgc cgagcgcgcg agcaccaggc cgtcgacgtg cgtgaccgtc
    13681 acgcagatgt cgctgtcggg cgcggcgctc aacgcccctg cggcgacgga tccatgcagg
    13741 tagagggttc ccccctcgtc gggtccgtcc gcatcgaccc cgaaagccag cggcagcacg
    13801 gcggggtggc caccgaggat caccccgacg tggcagacga gggcatcgcg gaggacggca
    13861 aacagctgct cgcgttgggt cgccgctcgc tctcgatgcc ggtgcacctc ggtgcgacgt
    13921 gtcggggaga gccgcaggtc caggacggag tcgagcgtca tgagcccagt ctggccgaaa
    13981 agtggcctgg cgacacgggc caatcgccgc taatctcgaa aggccaattt ggagcggtgt
    14041 ggaggtggcg agtgacgact gcgacgccgt tggcggtgca cctggaccgg tcaggcgacc
    14101 aagcgctgcc ggtgcagctg gctcggcaga tccgcgccct cgtcgccgac ggttcgatcc
    14161 gccccggcca gcgactgccc tcctcgcggg cgttggcccg cgagctccag gtggcgcgag
    14221 cggtcgtgga ggccgcgttc gaccagctgc tcgcggaggg ctgggtcgtc tcacggcaag
    14281 gtgcggggac gttcgtgcag gacgtgggct cccgtgctcg ccggggagac gtggcgcgga
    14341 cgacgccgga cgccgtggcc gccgaaggcc acgagctgat cagcttcgac acggggacgc
    14401 cgtacgtcga cccgcggttg aagtccgggt ggcgccgtgc ctggcgcgag atgtcgatgg
    14461 ccgaaccgcc gcgcggctat cccgagccga ctgggatacc ggagctgcgc gagctgttgg
    14521 cgacgtacgt cggccaccgc cgcggggtca cctgctcgcc ggaggaggtg ctggtcaccg
    14581 caggaaccac gcacggtctc ggcctggtgc tgtcgacgct gcggcccggc gcggtcgccg
    14641 tcgaggatcc gggctaccgc gccgcagtgg agatctccca ggccagtggt ttcgacgtcg
    14701 tcgacgtgcc ggtcgacgac gagggactcg acgttcgaac gctcgcagtc agccgtcgca
    14761 gggacatccg ggctgtctac gtgacgcccg cccaccagca ccccacagga gtgacgctct
    14821 ccgcgagccg gcgtcttgca ctcgtcgcgg aggccaggcg ccgagggtcg ctgatcgtgg
    14881 aggacgacta cgactcggag ttccggtacg acgtggcgcc gctgcccgcc ctcgcgtcgc
    14941 tctcgcggga ggtcgtctac ctggggacag cggcgaagtc ggtacacccg gcgttacgga
    15001 tcggatggct ggtcggaccc gccgagctgg tcgccgcgat cgcgcagcaa cgtgcggtac
    15061 gccgcgacca tccgagctgg cccgtccagc gagcgttcct ggcgatgctg cgcgacgggc
    15121 acgtcgaccg gctgatccgc tctgcacggc gcgtgtatgc cgctcgcagc gaggccgtct
    15181 cgcgtcgtct cgcggcatac gggccggtgc agggatccgg cgcggggatg tacgtgaccg
    15241 tgacactggc agaggaggcg gcgcggcgtg tctctgccgc cgcccggaag gctggtttcg
    15301 acgttcccgt cctgtccctg tacgctcgca cgcaccgccg ccacggcttg gtgctgggtt
    15361 tcggcggtgt caccgacgac gagctcgacc gatgtctgga cgtggtggag agcacgctct
    15421 cccgcacgca ggcgatggct tgagcacgtt gcggtccatg tcacaataga ggcgcggcat
    15481 cttgacatga ccgggctttg ctgcgccccg gaagcaatct aagagaggac gttcgtgtcg
    15541 tcgagcacgt cccgcaccct tcccgagtcc ctgctctctc atccgtcggt gatgcgcctc
    15601 atcgcccgag ggcaacacca cggacaggtc tccgccaacg acatccgtga cacgtgcgac
    15661 gaggccgaca tcacggccgc gcagcgtaag gcgctgctga agcacctcgc cggtcacggc
    15721 gtgagcgtcg cggtcggtgc tgaggacgcg gccggtcgca ggaagacggt cgccgccgtc
    15781 gccaagcgcg cgaccgtgtc gactgccacg aagaagacgg ccgccaagaa agcgcccgcg
    15841 aagaaggcaa ccacgacgaa gtcggcggcc aaaccggcga gcaggtccac gacgaaatcc
    15901 ggagacggca cggccaggaa gccggctgcc aagaaggccg ccacggtgac gaagacgtcc
    15961 agccggtcca caccggtcaa gcagacgagc gccaagaccg aggccaagcc ggtcgccaag
    16021 aagacgacgg ccaaggcaac cgcgaagaag gcagccgcaa cgaagaaggc gccggcgacg
    16081 agcgcagcga acaagccggc cggtacaccg gtcgagaagg attccgtcga gcgggtcgag
    16141 ccgatggtcg agatcgaggc cgccgcgatc ctcctcgacg ccgacgtcga cggggacaag
    16201 aagacgctcg ccgacatcga cgactccgtg ttcgagaagg atctggagaa cgaccccacg
    16261 ctcaaggagg acgagaagga gggcttcacg ctctccgagg ccgacgaggc ggacgagccc
    16321 gagcagaccg tcacggtcgc cggcgccacc gcggacccgg tcaaggacta cctgaagcag
    16381 atcggcaagg tcccgctgct caacgccgag atggaggtcg agctggccaa gcgcatcgaa
    16441 gccggcctct tctcggagga gaagctcggt cgggagatga agctgtcggc gaagatgctc
    16501 gacgagctcg gttggatcgc cgaggacggt cgccgggcca agaaccacct gctcgaggcg
    16561 aacctgcggc tcgtggtctc gcttgcgaag cggtacaccg gccgcgggat gctcttcctc
    16621 gacctgatcc aggagggcaa cctcggcctg atccgggcgg tcgagaagtt cgactacacg
    16681 aagggctaca agttctcgac gtacgccacc tggtggatcc ggcaggccat cacgcgggcc
    16741 atggccgacc aggcgcgaac gatccgaatc ccggtgcaca tggtcgaggt gatcaacaag
    16801 ctcgcccgcg ttcagcggca gatgctccag gacctcggcc gggaacccac gccggaagag
    16861 cttgcaaaag agctcgacat gacgccggag aaggtcgtcg aggtacagaa gtacggccgt
    16921 gagccgatct cgctgcacac cccgctgggc gaagacggcg actcggagtt cggtgacctg
    16981 atcgaagact ccgaggccat cgtcccggca gatgcggtgt cgttcacgct gctgcaggaa
    17041 cagctgcacg ccgtgctcga cacgctgtcg gagcgcgagg ccggtgtcgt ctcgatgcgc
    17101 ttcggcctga ccgacgggca gccgaagacc ctggacgaga tcggcaaggt gtacggcgtc
    17161 acccgcgagc ggatccggca gatcgagtcg aagaccatgt cgaagctccg tcacccttca
    17221 cgttcacagg tcctccgcga ctacctcgac tgagttgtca gaaccacatg gagttatagc
    17281 cctctgtggt tctgacaact cggtagcgtc gggaccgtgc gagacacgaa ccgctggacg
    17341 cgctgtgccc atggacaccg gcactggggt tcgctgggcg cggctggcct gatgctcacc
    17401 cggcgtacgc cggacagtcc ggccgggaca ccggaggtct tgttgcagca tcgggcggac
    17461 tgggtgcatc acggcggcac ctggtcggtc cctggcggag ccatccagcg cgatgagacg
    17521 gcctgggaga cggcacttcg ggaagtacgt gaagagacag ggctcgacgt gggcgccgcg
    17581 cacgagctcg ctcggtacgt cgacgaccac ggcggctgga gctatacgac catcatcgcg
    17641 acgttggaca cgaacacggg agtctccgcg accctggcgc agatcggttg gctcgacacc
    17701 atcctgatcg gcgagcagca ggacctgagc tggcggccgg tcgctgccgt cgacgggctt
    17761 gcgctgcatc cgggtttcgt cgacagctgg cccgccgtcc gctcgctcgt cctcactacc
    17821 ccgacgcctg ctcctcggga gtgagcgccc ggaggacgca gaactcgttg ccgtccgggt
    17881 cggccagcac gacccaggag acgtcgtcac cttggcctac gtccgcgcgg gtggcaccga
    17941 gcgcgaggat ccgctcgacc tcggcggcct ggtcgtcggg tcgcaggtcg atgtgcaacc
    18001 ggttgatcac cgacttctcc tctgggacct ggaggaacaa caactcgggg gagacgccgt
    18061 cttctggact gtcggccggc ggtccgagcg ccacctcgtc cggggcgtcg tagatccgcc
    18121 gccagcccag ggtctgctcc cagaagggcg ccagccggcc gggatcggtc gtgttcacgc
    18181 acacgcactg ccatcgcagg gtcatggggc catcgaagca tcgaccgccg acagcctgca
    18241 cccggttatg gggccctggt cgcgccgcaa gtacgacgct cacgccgata ccgtgggccc
    18301 atggcttcga gctacatcaa gttcgtgacc gtggactgtg acgacccgat caaggtggcg
    18361 acgttctggg cggcggccct cgggtccaac ctcgacgagg acgccaccga cgagcgcgct
    18421 ttcgtggagg cgccgggttg gggcggtccg aacatgtggt tcgtgaaggt gccggagccg
    18481 aagtcggcca agaaccgcat ccatttcgac cttcgagcac caggcacgat ctccgacgag
    18541 gtggatcgcc tcacggcgct cggtgcgacc gtccagcacg tccgcgacga tctcgtggtc
    18601 atgcaggatc ccggcggcaa cgagttctgc gtggagtgaa ggtcgcagcc gtcaggccgc
    18661 gatgacagcg gcgagccagg tcccggcagc gaccaggctg cccgccagtt cgatgagcat
    18721 cgacaacccg acggccgtga tcgcctgccg ggtggacgtc cacgccgcct gtgggctgcg
    18781 gaggcggacg tgttcggcga catacacacc gagggggaac ccgatgaaca gccccaccac
    18841 aggaacgacg aagaagccca cgatcccgac caagccgccc accgtcaggc tcctggctgg
    18901 tacgccggcc gcaagcaacc gcttcgaggg gaccacgtac ttcacgacct gcgagactcc
    18961 gaccgagacc aaggcgacgg cgaagaccgc ccatccggtc ggcgatgcct cggcgaacgc
    19021 ccagaccccg atggcgccca acaccaacag ctgccccggc agcaccggca ccacgacgcc
    19081 gacgagcccg acgacgatcg caacaccgac caggaacgtc acggccgtcc tctccatcca
    19141 ggcggggtcc gaggacactg cttccgccca gacattccca gattgagccg gctagggcca
    19201 gacagaccca gaccggatac acacctgata cacaagcgtg gcggccacct ctcggtggcc
    19261 gccacggcac gtcatgaggg gcccggaggg gcccgaagga tcagcgctcg tcgtccggcg
    19321 cggtgctggg ggtgtccgcc aacttggcgg tctcgtcgtg gatcgacgct gccacgagct
    19381 gcaacttctc cgcgtgctct cgggcgtggt gggcgcagaa caacagttct ccaccgctca
    19441 ccagctcgac gcgcacgtac gcctgcgccc cacaacggtc acagcggtcc agcgccgtga
    19501 tctgcggact cggtgcaaca gctgtggtca cgggtgcctt cccttcttca ctggacatct
    19561 ccacaagaga caaccagtcg accaaggccg gtcattcccg atgtgacgtc catcgcttgt
    19621 cgagatgagc cggcgggtgc cgatcgtgat acctctgtga tctgccgtct tcgatcctgc
    19681 cgttccggtc ggacactacc tgcggccggg ttcaggtcga tgtccttact tcgacaccga
    19741 cacccgtgcg ggtgccctgc cggtcagcaa tctctgtcac taggacgcat cgtcgcccct
    19801 cctcgatgcc ggcggcggga gatgtaccca attcgtgacc cgcgtgtcgc tgaaaccatc
    19861 cgaacggttg agccctccgt ggcccgttcg atccgacggc gtggtggcga cggatctgtc
    19921 cctgccgccg ggtatcgttc gagccgtccc cgacaccggc gtacgccgcc cgcgaacgcc
    19981 cctgtcatca cccgtcatca cccgtcatca cccgtcatca cacagcatca cccggcatca
    20041 caaggagttc cgtgccgact cgaggccatg tcggcggcgc tgtcgacgtc gtcgacgcgt
    20101 cgtacaccgc tcgtcatctg ctcgtcctcg aggggctgga agcggttcgg aaacgtcccg
    20161 gtatgtacat cggctcgacc gacacccgcg gactcatgca gtgcgtctgg gagatcatcg
    20221 acaacggcgt cgacgaagcc ctggcgggag tctgcagcca catcacggtg agcctctatg
    20281 ccgacggttc ggtggaggtc cacgacgacg gacgcgggat cccggtcgac gtcgagccga
    20341 agaccgggct gtccggcgtc gaggtcgtct tcaccaagct acacgccggc gggaagttcg
    20401 ggggcggatc gtacgtcgcg acgggcggcc tgcacggtgt cggtgcgtca gtcgtcaacg
    20461 cgctgtccgc ccggctcgac gtggtcgtcg accgcagccc ggccagctgg gcgatgtcgt
    20521 ttcgtcgcgg agtcccgggc acgttcgacg gggaaggccc gcgggcggcg ttcacccgtc
    20581 gtgaaggcag catcacgaag gtgggcaagg tcaagaaggg cgtcaccggg acccgcatcc
    20641 gtttctggcc cgaccggcag gtcttcacca aggatgccgc ctactcctac gacgagctcg
    20701 tcacccgggc tcgccagacc tcgttcatcg tcccgggtct tgcgatctcg atccgggacg
    20761 agcgagcggc tgaggtcgtg gaagagacgt tccgtcacga cggcggcatc accgagttct
    20821 gtgagttcct ggcgacagac gagaaggtca ccgacgtgct gcgactgcac ggggtcgaca
    20881 acttcaccga gaccgtgccg gtgctcgacg acgccggcca catgacgccg caggacgtcg
    20941 agcgcgagct caccgtcgac gtcgcgttgc ggtggggcag cggttacgac accgagatcc
    21001 ggtcgttcgt caacgtgatc gccactccga agggcggcac ccacgtcacc ggcttcgaac
    21061 gagcgttgac gaagacgttc aacgatgtcg tccgcgccac ccgaggcatg ctgaaggcag
    21121 gagagtcgga cctggtcaag gacgacatcc tcgaagggct caccgcggtc gtgaccgtgc
    21181 gactggccga gccgcagttc gaggggcaga ccaaggacgt cctcggcacc cccgcggcct
    21241 cgcggatcgt ggccaagatc gtctccgcgc aggtcagggc gttcctgacg tcgacgaaga
    21301 agcaccagaa gccgcaggca cgggcgctcc tcgagaaggt cgtcaacgcc gctcgcaccc
    21361 gggtcgcggc tcgtcagcaa cgcgacacgc aacgccgcaa gagcgctttg gagtccagcg
    21421 ccctaccggc gaagctggcg gactgtcgga gcaacgacgt cgaccgcagc gagctcttca
    21481 tcgtcgaggg tgactcggcg ctggggacag ccaagctggc ccgcgactcg gagtaccagg
    21541 cactgctccc gatccgcggc aagatcctca acacccagaa gaagtcggcc ggcgagatgc
    21601 tccagaacgc cgagtgcgcg gccatcatcc aagtcgtcgg cgcgggcagc ggccgtacgt
    21661 tcgacctcga acaggcccgc tacggcaagg tcatcttcat ggccgacgcc gacaccgacg
    21721 gcgcccacat ccggtgtctg ctcgcaacac tgttccaccg gcacatgcac ggcctgctgg
    21781 cggccggtcg cgtgtacgcg gccgtcccgc cgctgcaccg gatcgagctg tccaatccga
    21841 agaagggcat ggacaggtac gtctacacgt actccgacga cgagctgcag cgaaaactcg
    21901 ccgagctgag caagaagaac gtccgctgga aggatcccgt ccagcgctac aaggggctcg
    21961 gagagatgga cgcgcaccag ctcgccgaga ccaccatgga tccccggcac cggacgctgc
    22021 ggcggatcac gatcgacgac gccgggagtg ccgagcagat gttcgagctg ctgatgggca
    22081 acgacgtcgg gccgcgccgg gagttcatcg tccaaggcgc ctacgagctc gacctcgaag
    22141 ccatcgacgc ctgacggctg accggtggtt cccgcgaatg cgggaaacgt ctgggttttc
    22201 gcgccccggc aacagaatct ttcccgcatt tccggggaag ccgtgacggc ttgttaccgg
    22261 ggggttgcac tttcagggat caacggcagg atgagactca cgacacagcc ctggggaggc
    22321 gctccatggc aacagccact cgttcctggc ggggcggcgg caggcaccgt cgccaccgat
    22381 ctctggccct catcgtcgtc ctcgcagccc tctcggcgct gctggccgct tgtggagggg
    22441 attctggcaa gaccacgttg tactggtaca tcaaccccga cgtcggcggg caggatgccg
    22501 tcgccaagaa ctgcagcacc gaccagtaca cgatcagcac cgaggtgctg ccgcagaatg
    22561 cgaccgacca acgagtccag ctggcaaggc ggctggctgc gcacgactcg gccatcgatc
    22621 tcatcagcct ggaccccccg tacaccgcgg agttctcgaa cgccggcttc ctcgcggcga
    22681 tccccaagga cgaacagcag cgcctggccg accagtcctt cgggggtgcc acgaaggcag
    22741 ccacctggga cggccagatc gtggccttcc cgttctggtc caacacccag gtgctgtggt
    22801 accggaagtc gttcgtggag aaggccggca tcgacatgag caagccggtg acctgggacg
    22861 agctcatcga cgtcgccagc aagaacggcg gccagctcgg cgtccaggcg aacaagtacg
    22921 aaggctatgt cgtctggatc aatgcgctgt acgccggcgc gggaggacag ctcgtctcca
    22981 acaccgagcg aggcgccgac ctgaccatcg acatcgactc cccggcgggt gacaaggctg
    23041 ccgccgtcat ccagaagctc tcgtcgtcgc cggcagcgcc gcccgacctt tcggtgtcca
    23101 acgaaggcac cgccggatcg acgttcggcg gcccggctgg ctcgttcatg gtcaactgga
    23161 cctacatctt ccacaactac gacgcgactg atccggacgt cgccaaggac atcggataca
    23221 cgcgctaccc ggagacggtg gcaggcgagc agtccggacc gccgtacggt ggcatcggca
    23281 tcgtcgtgag tgagtactcg tcgcacaagg acttcgcctt gcaggcggcc gcgtgcttgg
    23341 tttctccgaa gaaccagggc atcaatgccg agatcacggg caacatgccg gcgagcgcag
    23401 ccggctacca gtacccgcca ctccagaagc tctacccggc cgacctgctc gcactgttcc
    23461 agcagagtgt cgagacatcc gcgcccaggc cgtccacgcc gtactggagt gacatctccg
    23521 gctccatcca gagcacgtgg cacccgcccg actcggtgaa ccaggacacg ccgaagaaat
    23581 cggccgcctt caccgagcag gtgatcaacg gagagaggct gctatgacca ccacgaccac
    23641 cgcggccccc ccgaccaaac gggccacgaa gaaaccaacg agcgaccggt cagtagccga
    23701 gaaccggttg ggtcgacggc tggtcgcccc ggccgtgatc atgatgctga tcgtgacggc
    23761 atggccgatg atcaaggcgc tctacgactc gacgtaccgc taccggctca ccgcgccgga
    23821 cgacaaggag ttcgtcggcc tcggcaacta cgcgaccgtg ctgacggact cactgttctg
    23881 gcgggacacc tggaacaccg tgatcatcat ggtggtcaca gtcgcggtcg aactcgtgat
    23941 cggcttcgcg ttcgccatgg tcatgcaccg gatcatcttc gcgcgcggcg tgatgcggac
    24001 cgcgatcctc atcccgtacg gcatcgtcac ggtcgtgtcg gcgttcgcgt ggcagttcgc
    24061 gttcagcatc aacaacggct tcgtgaacga ctggttcagc tggctgccat ggatcagccc
    24121 tgacacgaac tggttcggct cacactggcc ggcgatgttc gcgatcatga tctcagagat
    24181 ctggaagacc acaccgttca tggcgttgct gctgcttgcc ggtctgtccc agatctccga
    24241 ggacatgctg gaggcggcga aggtggacgg cgccacctgg tggcagcgtc tgtacaaggt
    24301 gatcctgccg aacatgcgcg ccgcgatcat ggtcgcagtg ttgttccgcg cgttggacgc
    24361 cttccgcatc ttcgacaaca tctacatcat gacgaagggc gcccagaaca ccgagtcctc
    24421 gtcgttcctc acgtacagac aggtgatcga acagatgcag ctcgggctgg gctccgcgct
    24481 ctcggtgctg ttgttcctca ccgtgctgtt gcttgcgggc atcatcgtca agctgttcaa
    24541 ggtggatctt gcccaggcga ggggtcagtc atgaggaaca agagtgggat cggggtgtgg
    24601 gtcggcttct tggcgatcat gatctggtgc ctcctgccgg tcgcctggat catctcgttg
    24661 tcgttcaagt ccgtggcaga aacgacggtt ggcagtcccc acttcctccc cgacgatccg
    24721 acctggcaga actacaagga catccttcag aacgacgact tccagctcgc cttgcgcaac
    24781 tccttcggga tcgcgttcat ctccaccgca ttgtcggtgg tcctggccac gttggcggcg
    24841 tacgcgatcg cccggctgga gttcaagggc aagcgtttcg tgttgtcgac cgctctggcc
    24901 atcgcgatgt tcccggtcgt cgcgctcgtg ggtccgctgt tcgacatgtg gcggacgttc
    24961 gggctgttca acacgtggct cggcctgatc atccc
//
LOCUS       DATBFZ010000453         2338 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_3271322, whole genome shotgun sequence.
ACCESSION   DATBFZ010000453 DATBFZ010000000
VERSION     DATBFZ010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3271322"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(150..551)
                     /gene="sodN"
                     /locus_tag="VH419_13845"
     CDS             complement(150..551)
                     /gene="sodN"
                     /locus_tag="VH419_13845"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase, Ni"
                     /protein_id="HEY7044747.1"
                     /translation="MLARFFAPTIDVSAHCDLPCGVYDPAQARIEAESIKAIIGKLAD
                     NDDPDFRTRAIIIKEQRAELVKHHLWVLWTDYFKPPHFEKYPQLHTLFNEATKLAGAT
                     GVKGSVDAAKADELLAKIDEIDKIFFETKQA"
     gene            675..1001
                     /locus_tag="VH419_13850"
     CDS             675..1001
                     /locus_tag="VH419_13850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020575799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HEY7044748.1"
                     /translation="MTAAARRRLGLAVVRGRSMEPTLREGDRLLVLYGATPQPGGLVV
                     VRLPDRPVAVKRAARRTSEGWWVERDNPAEGVDSWLVGAIADDDVIAVVLLRFWRWRW
                     VRRLRG"
     gene            1055..2284
                     /locus_tag="VH419_13855"
     CDS             1055..2284
                     /locus_tag="VH419_13855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012922764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HEY7044749.1"
                     /translation="MATAPGTRRDRESEERVPPAPAPHPESDARWHAGDPVFELHRGG
                     KIRVESSVPLVDADDLSMAYTPGVARVCQEIVAHPDSARDLTWVSNTVAVVTDGTAVL
                     GLGDIGPVAALPVMEGKAVLFKEFGGVDAVPICLDCTDVDDLVETVVRLAPSFGGINL
                     EDISAPRCFEVERRLKERLDIPVFHDDQHGTAVVVLAALRNALRVVGKTLGDVRIVVS
                     GAGAAGVAVTTILREAGARRIAVLDSLGIIGPDRDDLTDVKQALADETNAEGLSGDLS
                     DAMGGADVYIGVSGGEVPEPDVAAMADDAIIFALANPDPEIHPDVAHTYAKVVATGRS
                     DFPNQINNVLAFPGIFRGAFDAGAREITEDMKLAAAGALADLVDSPDPAAIIPSPFDA
                     RVAPVVAAAVAAAAPRS"
BASE COUNT          355 a          751 c          837 g          395 t
ORIGIN      
        1 ctcgtcgcac tgctcgccgc tcctgccgtc gtggtgctcg tgatcgtgct gatgccgtcc
       61 acgacggtcg cgctgtacgg gcaccggctc agcgacaccg aacccgagtg acggcccaac
      121 cgcggtgaag agtcgctccg atggggtgat caggcctgct tcgtctcgaa gaagatcttg
      181 tcgatctcgt cgatcttggc cagcagctcg tcagccttcg ccgcgtccac gctgcccttg
      241 acgccggttg caccggccag cttggtggcc tcgttgaaga gcgtgtgcag ctgcgggtac
      301 ttctcgaagt ggggtggctt gaagtagtcg gtccacagca cccacaagtg gtgcttgacg
      361 agctcggcgc gctgctcctt gatgatgatc gcgcgggtcc ggaagtccgg gtcgtcgttg
      421 tcggcgagct tgccgatgat cgccttgatc gactcggcct cgattcgagc ctgcgccggg
      481 tcgtagacac cgcacggcag gtcgcagtgg gccgagacgt cgatggtcgg tgcgaagaag
      541 cgagccagca tgaactggtc ctttctgtag caggctggag aggtcgtcac actgagtctg
      601 cgccggggaa ttgtgcgcaa ccacagcaga ccctggactt gttcccgacc ctactccgct
      661 gaaacgtagg tgccatgact gctgcggccc gccggaggct gggcttggcc gtcgttcgcg
      721 gccgctcgat ggaacccacg ttgcgcgagg gcgatcgact gctcgtgctg tacggcgcca
      781 caccgcagcc cggcgggctc gtcgtcgtac ggcttcccga ccggccggtt gcggtgaaac
      841 gggcagctcg gcgtacgtcc gaaggctggt gggtcgaacg tgacaacccc gccgaggggg
      901 tcgactcgtg gctcgtcggt gcgatcgcgg acgacgacgt catcgcggtc gtgctcctca
      961 ggttctggag gtggcgctgg gtgaggcggc tgcgaggatg atgctgctgg acgttgaacg
     1021 cacggcagag acgaccggag acaggtgacc atcgatggcg acggccccag ggacacgacg
     1081 tgaccgcgag tccgaagagc gcgtgccacc cgcacctgca ccgcatccgg agagcgatgc
     1141 gaggtggcac gccggcgacc cggtgttcga gctgcaccgc ggcggcaaga ttcgtgtcga
     1201 gtcgagcgtg ccgctggtgg atgccgatga cctgtcgatg gcctatacgc cgggggtcgc
     1261 gcgggtctgc caggagatcg tggcgcaccc agactcggcg cgagacctca cgtgggtgtc
     1321 gaacaccgtc gccgtggtga ccgacgggac ggccgtgctg gggctcggcg acatcggtcc
     1381 ggtggccgcg ctgccggtga tggagggcaa ggctgttctc ttcaaggagt tcggtggcgt
     1441 cgacgccgtg ccgatctgtc tggactgcac cgatgtcgac gacctcgtgg agaccgtcgt
     1501 ccggctggca ccgagcttcg gcgggatcaa cctcgaggac atctcggcgc ctcggtgctt
     1561 cgaggtggag cgccggctca aggagcgtct cgacatcccg gtcttccacg acgaccaaca
     1621 cggcacagcg gtggtggtgc tggcggcgct gcgcaacgcc cttcgcgtcg tcggcaagac
     1681 cctgggcgac gtacggatcg tggtgtcggg agccggagcg gccggcgtcg ccgtcacgac
     1741 catcctgcga gaagcgggtg cacggcggat cgccgtcctc gacagcttgg ggatcatcgg
     1801 tccggaccgc gacgacctca ccgacgtgaa gcaggcactg gccgacgaga cgaacgccga
     1861 gggactctcg ggagacctgt ccgacgcgat ggggggcgcg gacgtgtaca tcggcgtgag
     1921 cggcggcgag gtcccggagc cggacgtggc ggcgatggct gacgacgcga tcatcttcgc
     1981 gttggcgaac cccgaccctg agatccatcc cgatgtcgcg cacacgtacg ccaaggtggt
     2041 cgcgaccggt cggtcggact tcccgaacca gatcaacaac gtgctggcct tccccggcat
     2101 cttccgtggc gccttcgacg ccggcgcccg cgagatcacc gaggacatga agctggcggc
     2161 cgccggcgcg ctggccgacc tggtcgacag cccggatccg gcggcgatca tcccgtcccc
     2221 gttcgatgct cgggtggcac cagtcgtggc tgccgccgtg gccgctgcag caccccgatc
     2281 ctgacgtcgg cgtggtccca gcgcgcgccc tagggtggac agatgttcgc cgcgtacg
//
LOCUS       DATBFZ010000454         4099 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_2940952, whole genome shotgun sequence.
ACCESSION   DATBFZ010000454 DATBFZ010000000
VERSION     DATBFZ010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 4099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4099
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2940952"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(487..3633)
                     /locus_tag="VH419_13860"
     CDS             complement(487..3633)
                     /locus_tag="VH419_13860"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEY7044750.1"
                     /translation="MKPVADRTLASLKDFQRATVDYVFDRLWLAEDQVKRFLVADEVG
                     LGKTMVAKGVIARTVEYLWDTDKRIDIVYICSNSQIARQNLSRLNVVGGFEVRHADRL
                     TLLPRVIRNLRDQRVNFVSFTPGTSFQVGSSGGAYPERVLLYWMLAACWGSNVTGVAG
                     WKRFFEGGMSHDNFESQLAGFDRTWLDEDLCQSFADAIANATGPAGRPLREELEDCAL
                     EFNYLRGKPEGDLGWRRNRLVGKLRQLVARAAVVHLEPDLVILDEFQRFKHLLDSDDE
                     GARLANAIFDYPDAKVLLLSATPYKMYTLPDEPEGDDHYRDFTRTVRFLAGDERAHIV
                     ERELRAMREGLLAGGDHTRARYARNRVEHELRRVMSRTERLSSTPDRNGMLVERPLDG
                     VQLAAEDLRSWRTFDDIARQIDRHDVFEYWRSTPYPLNLMERNSYQVRTKFQAAAERN
                     DPALRAMLQRARGLLDWEEIRRYHQVDPGNAKLRGLTHDVLDRGAWKLAWLPPSLPYY
                     ETAGAYALPPLRNFTKRLIFSAWSVVPKAVAAMLSYEAERRAIEAAGYSDRHYDDRPI
                     TPPLQFRLNGGRPAGMPALALLYPGLILARLGDPLEVARTLGTSMPLPRQALLDAVRE
                     RIQGVLTRLPEGSPRIGTPDQRWYWAAPFLLDRRIAAGDNYEFLSRMRAWDDPDDDDQ
                     ESRLAAHLRTADGVRELKLGPRPDDLDEVLTKMAIAGPGVCALRALSRATGGADALKD
                     AGFRNSAYRIAQGLRSLFNKPEIIVLLRSGEDESYWRAVLDHSVDGCLQAVLDEYVHV
                     LIESEGLQDADAHRRARTLTETMTEALSTRTAANTVEQVRVIDGEVHLDQHRISSHFA
                     ARYGRAQSSDQAAMREATIRTAYNSPFRPFVLASTSVGQEGLDFHTYSHAIVHWNLPG
                     NPVDLEQREGRVHRYKGHAVRKNVAAEYGPAALNPESCDPWSAVFSAAQAAGSDEESD
                     IKPFWIFTRPDGAVIERYVPALPLSREAQQYRRLLRTVGAYRLVIGQPRQDDLIRYVG
                     EDISWLRINLTPGLPNGA"
     gene            complement(3630..>4099)
                     /locus_tag="VH419_13865"
     CDS             complement(3630..>4099)
                     /locus_tag="VH419_13865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044751.1"
                     /translation="WTIAPVNVTPFIAVETTCGTGAARVTRRCVLKASLNGAINGRRQ
                     DAVFSILRNKEDVLRYLVFLLGDPSYDALFAQLAGVDSERFADSDRPVLTSADVALFE
                     PLVRATGRDEDALARVASLVEELRELPNGHELVPDGFDELWEVVWQVHQEGRA"
BASE COUNT          634 a         1195 c         1484 g          786 t
ORIGIN      
        1 gcgatctcgc tgttgctgcg acccgcgtgc cagtggacca ggatctcgat gatgtcgacc
       61 acgtcgaacg ttctcctcgc catcgggcct cccgccccga acgcccgggt tgagaggaat
      121 gctgctcttg ccctgatgac caggtccagc ccgacacgct cgcgctcccc atggggaatt
      181 acgtgatcgg gctggggaat tacgtgatcg ctaccggcct gcctgcggga atttcgtgat
      241 cgtcaacagc cgggactccg catctgttcg gctatggtca cggagtgtta taatcgctcc
      301 tcctgcgcct gccagatcgc cgtcgatgag cacggctagg tccgcgagcg ccgcgtcggc
      361 caggcctgca gcgacctaga ctgtgcccgc cgccggtgct ggatccaaga aacgcacgtc
      421 gagctgaccg gcctgctgag cgaagggtcg gcccagagat cagttcgaag gctgggtgtg
      481 cgttgctcaa gcgccgtttg ggaggcctgg tgtcaggttg atgcgaagcc aggagatgtc
      541 ctcgccgacg tagcggatca gatcgtcctg gcgtggttgc ccgatgacga ggcggtatgc
      601 gccgacggtg cggagcaggc ggcggtactg ctgggcttct cggctcagtg gaagtgcggg
      661 gacgtagcgc tcgatgacgg cgccgtcggg tcgtgtgaag atccagaagg gtttgatgtc
      721 ggattcttcg tcggagccgg cggcctgcgc cgcggaaaag acggctgacc aggggtcgca
      781 ggattcgggg ttgagggctg ccgggccgta ctcggctgcg acgttcttcc tgactgcgtg
      841 acccttgtag cggtggactc gtccttcacg ctgttccagg tcgacgggat tgccgggaag
      901 gttccagtgg acgatggcgt ggctgtaggt gtggaagtcg agaccttcct ggccgactga
      961 ggtggaggcc agcacgaagg gccggaacgg cgagttgtag gcggtccgga tggtcgcctc
     1021 gcgcatggcc gcttggtcgc ttgattgggc gcggccgtag cgggcagcga agtggctgct
     1081 gatgcggtgc tggtcgaggt ggacctcgcc gtcgatcacg cgcacttgct cgaccgtgtt
     1141 cgctgctgtt cgcgtcgaca gtgcctcagt cattgtctcg gttagcgtcc gcgcgcgtcg
     1201 gtgggcatcg gcgtcttgga ggccctcgga ctcgatcagg acgtggacgt actcgtccag
     1261 gactgcttgc aggcagccgt cgactgagtg gtcgagtacc gctcgccagt atgactcgtc
     1321 ctcgcctgag cggagcagga cgatgatttc tggcttgttg aagagggagc gaagaccctg
     1381 agcgatccga taggccgagt tgcggaaccc ggcgtcttta agtgcgtcgg ctccgccggt
     1441 tgcgcgggag agggcgcgta gggcgcagac gccggggccg gcgatggcca tcttggtgag
     1501 tacctcgtcg aggtcgtccg gtcgtggacc gagcttcagt tccctgacgc cgtcggctgt
     1561 gcgtaggtgg gcggcgagtc tggattcctg gtcatcgtcg tcagggtcgt cccaagcgcg
     1621 catgcgtgac aggaattcgt agttgtctcc ggccgcaatc ctccggtcta ggaggaacgg
     1681 cgctgcccag taccaccgtt gatctggtgt tccgatgcgt ggtgaaccct cgggaagcct
     1741 ggtcaggacc ccttgaatcc gctcgcggac ggcgtctagc agagcctgcc gggggagagg
     1801 cattgatgtg ccgagggtgc gtgcaacctc gagcgggtct ccgagccggg cgaggataag
     1861 gcctgggtag aggagagcga gcgcgggcat gccggctggt cggccgccgt tcaggcggaa
     1921 ctgcaatggt ggcgtgatgg gccggtcgtc gtaatgccgg tcactgtatc cggctgcctc
     1981 gatggctcgc cgctcagcct cgtagctgag catggcggca acggccttgg gcacgaccga
     2041 ccatgccgag aagatcagcc gcttggtgaa gtttcgcagc ggtgggaggg cgtaggcgcc
     2101 tgcggtctcg tagtagggaa gggaaggcgg aagccacgct agcttccacg cgcctcggtc
     2161 gagcacgtcg tgggtgaggc cgcgcagttt ggcgttgccg gggtcgacct ggtggtagcg
     2221 gcggatctct tcccagtcga gcaggcctcg cgctctttgg agcatcgcgc ggagtgccgg
     2281 gtcgttgcgt tcggctgctg cttggaactt ggtgcgcacc tggtagctgt tgcgctccat
     2341 gaggttgagc ggatagggcg tggagcgcca gtactcgaag acgtcgtgcc ggtcgatctg
     2401 ccgggcgatg tcatcgaagg tgcgccagga ccgcaggtct tcggcggcga gttgcacgcc
     2461 gtccaggggg cgttcgacta gcatgccgtt tcggtccggt gtggacgaca gacgctcggt
     2521 gcggctcatc acccggcgta gttcgtgctc tacccggttg cgggcatagc gagcgcgggt
     2581 gtggtcgcct ccggctagga ggccttcgcg catcgcgcgg agctcgcgtt ctacgatgtg
     2641 ggcgcgctcg tcgccggcga ggaagcggac ggtgcgggtg aaatcgcggt agtggtcgtc
     2701 gccctcgggt tcgtcgggga gcgtgtacat cttgtacggc gttgccgaca gcagcagaac
     2761 cttcgcgtcg gggtagtcga agattgcgtt ggcgaggcgt gcgccttcgt cgtcggagtc
     2821 gaggaggtgt ttgaagcgct ggaactcatc gagaatcacg aggtctggtt ccagatggac
     2881 caccgcggcg cgggcgacga gctggcgcag tttgccgacg aggcgatttc gacgccaccc
     2941 gaggtcaccc tcgggtttgc cgcgcaggta gttgaactcg agtgcgcagt cttccagctc
     3001 ttcccgcagc ggccggccgg caggacccgt ggcattcgcg atagcgtcgg cgaacgactg
     3061 gcacaggtcc tcatcaagcc aggtgcggtc gaacccggcg agctggcttt cgaagttgtc
     3121 gtgactcatg ccgccttcga agaaccgctt ccagccggca acgcctgtca cgtttgagcc
     3181 ccagcaggca gcgagcatcc agtacagcag cacccgctcg gggtaggcgc cgcccgagct
     3241 gccgacctga aacgaggttc ccggggtgaa ggagacaaag ttgacgcgct ggtcgcgcag
     3301 gttccggatc actctcggca ggagcgtcag gcggtcagcg tgcctgactt cgaaaccgcc
     3361 gacgacgttg aggcggctga ggttctggcg tgcgatctgg ctgttggagc agatgtagac
     3421 gatgtcgatg cgcttgtcgg tgtcccacag gtactcgaca gtcctggcga tgactccctt
     3481 ggcgaccatg gtcttgccca ggcccacttc gtcggccacg aggaacctct tgacctggtc
     3541 ctcggcaagc cagaggcggt cgaagacgta gtcgacggtt gctcgttgga agtccttgag
     3601 gcttgccagt gtgcggtctg cgacgggctt catgcgcggc cctcctgatg aacctgccag
     3661 acgacctccc agagttcgtc gaacccgtca ggtaccagct cgtgcccgtt gggaagctcc
     3721 cggagctcct cgacgaggct ggccacccgc gcgagcgcgt cctcgtcccg gccggttgcc
     3781 cggacgagcg gctcgaagag cgcgacgtcc gcgctcgtca gcacgggtcg gtccgagtcg
     3841 gcgaaccgtt cggagtcgac cccggcaagc tgggcgaaga gtgcgtcata gctcgggtca
     3901 cccagcagga aaacaaggta gcggagcacg tcctccttgt tgcggaggat ggagaagacg
     3961 gcgtcctgac gtcggccgtt gatggctccg ttcagcgacg ccttgaggac gcaacggcgg
     4021 gtaaccctgg ccgctcccgt accgcaggtg gtctcgacgg cgatgaacgg tgtgacgttg
     4081 actggtgcga tggtccagg
//
LOCUS       DATBFZ010000455         1537 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_3598237, whole genome shotgun sequence.
ACCESSION   DATBFZ010000455 DATBFZ010000000
VERSION     DATBFZ010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3598237"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="VH419_13870"
     CDS             <1..272
                     /locus_tag="VH419_13870"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEY7044752.1"
                     /translation="AREHLPVTFVILDNARYAAVDILASTDNAKMPGVDLGGIDFGKL
                     ATGMGCQAFRVAHPDELKPALTAALTDDRPTVVHVLVDPNPPALY"
     gene            complement(283..1164)
                     /locus_tag="VH419_13875"
     CDS             complement(283..1164)
                     /locus_tag="VH419_13875"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017934604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY7044753.1"
                     /translation="MPTMLEVRDLVHQYGTGPGAHLAVSGLTFSVDEDQLSCIVGPSG
                     CGKSTLLRCIAGLIKPTDGSVTLHGDAVHGVPEDLAVVFQDYSRSLFPWLSVRGNVEF
                     PLRTRVSGRGTRRTRAMEALEWVGLADAASKYPWQLSGGMQQRVSIARALASQPALLL
                     MDEPFASVDAQTRAELEDLLLRVRREHGSTVLLVTHDIDESVYLGDRVLVLSRSPAKI
                     VADLTVDLPAQRDQITTRGSSTFVDLRTEIARLLHDKQSGQTGPTNGTSHTSDEALAA
                     ERFLAAEREEASRQSTR"
     gene            complement(1166..>1537)
                     /locus_tag="VH419_13880"
     CDS             complement(1166..>1537)
                     /locus_tag="VH419_13880"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY7044754.1"
                     /translation="VEGVRSVDSVKTDTARSFRTPRGQWVTMVVLPAALPKIFAGLRL
                     SLAISVILMVVSEMVGTTSGIGYQLDHARAQFDFTTMWAWIVLLGIIGYGLNLALLTV
                     ERRVLRWQPTRAAVAAAKAGG"
BASE COUNT          237 a          567 c          526 g          207 t
ORIGIN      
        1 cggcccgcga gcacctgccg gtcaccttcg tgatcctgga caacgcccgc tacgcggcgg
       61 tcgacatcct ggcgagcacg gacaacgcga agatgccggg cgtcgacctg ggcggcatcg
      121 acttcggcaa gctcgccacc ggcatgggct gccaggcctt ccgggtggcg catccggacg
      181 agctgaagcc cgccctgacc gccgccctga ccgacgaccg cccgacggtg gtccacgtcc
      241 tggtcgaccc gaacccgccg gcgctgtact gaccccggcg ggtcagcgcg tcgactgcct
      301 gctggcttcc tcccgctcgg cggccaggaa gcgctccgcc gcgagggcct cgtccgacgt
      361 gtgggacgtg ccgttcgtgg gaccggtctg gccggactgc ttgtcgtgca ggaggcgggc
      421 gatctcggtg cgcaggtcga cgaacgtcga cgagccgcgg gtcgtgatct ggtcgcgctg
      481 cgcgggcagg tcgacggtca ggtcggcgac gatcttcgcg ggggagcggg acagcacgag
      541 cacccggtcg cccaggtaga cgctctcgtc gatgtcgtgg gtgaccagca gcaccgtgct
      601 gccgtgctcg cggcgcaccc gtagcagcag gtcctcgagc tcggcgcggg tctgcgcgtc
      661 caccgaggcg aacggctcgt ccatcagcag cagcgccggc tgcgaggcga gcgcccgcgc
      721 gatcgacacc cgctgttgca tgccgccgga gagctgccac gggtacttcg acgccgcgtc
      781 ggcgaggccg acccactcca gcgcctccat cgcccgcgtc cgccgcgtgc cccggcccga
      841 cacccgcgtg cgcagcggga actcgacgtt gccgcgcacc gacagccacg ggaacagcga
      901 gcggctgtag tcctggaaca cgaccgcgag gtcctcgggc accccgtgca ccgcgtcgcc
      961 gtgcagcgtg accgagccgt cggtcggctt gatcaggccg gcgatgcaac gcagcaacgt
     1021 cgacttgcca cacccggacg ggcccacgat gcacgagagc tggtcctcgt cgacgctgaa
     1081 cgtcaggccc gacaccgcca gatgcgcacc cggaccggtg ccgtactgat gcaccaggtc
     1141 gcgcacctcg agcatggtcg gcatatcacc ctcctgcctt cgcggccgct accgcggccc
     1201 gtgtcggctg ccaccgaagc actcggcgct cgacggtgag cagcgcgagg ttcagcccgt
     1261 agccgatgat gccaagcaac acgatccacg cccacatggt ggtgaagtcg aactgcgccc
     1321 gggcgtggtc gagctggtaa ccgatgccgc tggtggtccc gaccatctcg gacaccacca
     1381 tcaggatgac cgagatcgcg agcgaaagcc gcagcccggc gaagatcttc ggcagcgccg
     1441 ccggcagtac gaccatcgtg acccactgcc cgcgcggcgt gcggaacgag cgcgcggtgt
     1501 cggtcttcac cgagtcgacc gagcgcacgc cctcgac
//
LOCUS       DATBFZ010000456         5670 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_653779, whole genome shotgun sequence.
ACCESSION   DATBFZ010000456 DATBFZ010000000
VERSION     DATBFZ010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 5670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5670
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_653779"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..786)
                     /locus_tag="VH419_13885"
     CDS             complement(<1..786)
                     /locus_tag="VH419_13885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020388617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEY7044755.1"
                     /translation="MRNGETTPVYSYADAIRQSVWVLAPDLDHNGQADRVAVDIIRPA
                     ELDGTAQIPVIMDASPYYACCGRGNESELKEYDADGNPTKFPLFYDNYYVPRGYAYAA
                     VDMAGTNRSTGCVDEGAASDILSVKAVVDWLNGRADAVDSAGDPVTAYWTNGDSGLIG
                     KSYDGTLANGVAATGVEGLKTIVPVSAISSWYDYDRYQGLPFSYDYPSWLSSVVELDR
                     TEPVDCSAINAQMAAEDGDETGAYTRFWSRRDYRAAPLPKASAV"
     gene            complement(1121..1507)
                     /locus_tag="VH419_13890"
     CDS             complement(1121..1507)
                     /locus_tag="VH419_13890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020580136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY7044756.1"
                     /translation="MTSKFTELAIDCADPNGLARFWCSVLDYEVQDEDAGVVTIGSPM
                     VPEGKNRPGPVPPTLTFARVTERKTVKNRLHLDVNPTDREQDEEVRRLLDLGARHADV
                     GQGEVSWVVLADPEGNEFCVLASRRP"
     gene            1618..1986
                     /locus_tag="VH419_13895"
     CDS             1618..1986
                     /locus_tag="VH419_13895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044757.1"
                     /translation="MTAYAPPGWPEQVRPPGAPWWEDTAVGWLLDQCPPDYRMYQGLR
                     RHPVVLARFAALHTEASIQAARRGISEARTSLRDVLPTESVEGAIAMWEREGARLLRV
                     RRAVGLIEEAFRGRQFRAKL"
     gene            2195..4117
                     /gene="dxs"
                     /locus_tag="VH419_13900"
     CDS             2195..4117
                     /gene="dxs"
                     /locus_tag="VH419_13900"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020575734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEY7044758.1"
                     /translation="MGMLDRIEGPSDLRDLDEQQLRELADEIRTFLITTVSKTGGHLG
                     PNLGVVELTLALHRTFDSPHDRIVFDTGHQAYVHKILTGRADQFETLRQQGGLSGYPR
                     RSESEHDIVENQHASTSLSYGAGLAAAYSMQGDDRYVLTVIGDGALTGGMAWEALNNI
                     AAAKHSRLVIVVNDNGRSYSPTVGGLAEHLAGLRTDPRYEQVLERIKRRLNRTKLVGP
                     PLYDALHAIKKGLKDALAPQGMFEDLGLKYVGPVDGHDRATLEQALDQAKKFAGPVLV
                     HAITRKGNGYPPAERHDEDNFHTLSAFDPSTGEVIAQSPTIWTDMFSDELVRLGEERK
                     DIVAITAAMLHPTGLHAFAAKFPDRTFDVGIAEQHAVTSATGMAMAGLHPVVAIYATF
                     ANRAFDQVLMDAALHGCGVTLVLDRAGVTGEDGASHNGMWDMSIFQVVPGLRIAAPRD
                     GTRVRELLREAVAVGDAPTVLRIPKGQVQSDIAAIETVDGVDVLYRQGERDVLVVAAG
                     AMAATCLDVAARLRAQGIGVTVVDPRWVKPVNPRLVTMAREHRLVVSVEDNGRVGGCG
                     AVLRQVLADAEVDVPTMVFGIPQEFLDHAKRSVILEAVGLGAQSLSRDITARVAGLDR
                     HESPSHTRADTSADPG"
     gene            4183..5667
                     /locus_tag="VH419_13905"
     CDS             4183..5667
                     /locus_tag="VH419_13905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEY7044759.1"
                     /translation="MSVFRTKSIEQSLKDSEDPEFQLKRSLTAIDLTVFGIGVIIGAG
                     IFTLTGQVAAETAGPAVSIAFVISAVCCAFAALCYAEFASAVPVSGSAYTFSYATVGE
                     LVAWIIGWDLLLELMLGASVVAQGWSQYAEQLLDEIGLGWPDSVGPGSHFNLLAFLLV
                     AVLCVLVTIGIKESMRVNMVLVTIKVFIVLFVIVMGISLINTSNYTPFVPDKIPATDN
                     SVDLTTTVWQALTGAPGQYGWTGVFAAASIVFFAFIGFDVVATTAEEAENPQRDLPIG
                     IIASLAICTVLYVAVALVITGMVDYPNIDPEAALASAFSDRGHGTVATIVSAGAVAGL
                     TTVVMTLLIGATRVTFAMSRDHLLPREFGHVNRTTRTPMIITIGIGVIVAATASLTPI
                     DKLEEMVNIGTLAAFTLVSIAVVILRRTRPDLDRPFKVPGAPFTPILSAVICVYMMLN
                     LTIETWLRFAIWMLIGFVIYFGYGIRKSRVGEAERAKSARRQPA"
BASE COUNT          882 a         1896 c         1927 g          965 t
ORIGIN      
        1 caccgcgctc gccttcggta gcggagccgc gcggtagtca cgtcgcgacc agaaccgcgt
       61 gtaggcgccg gtctcgtcgc cgtcctcggc agccatctgc gcgttgatcg cgctgcagtc
      121 gaccggctcg gtcctgtcca gctcgacgac cgacgacaac cagctcgggt agtcgtagga
      181 gaacggcagc ccctggtagc ggtcgtagtc gtaccacgag ctgatcgcgc tgaccggcac
      241 gatcgtcttc agcccctcca ctccggtggc ggcgacgccg ttcgcgagcg tgccgtcgta
      301 cgacttgccg atcagtcccg agtcgccgtt cgtccagtac gccgtcacgg ggtcacccgc
      361 cgagtccacg gcgtccgccc ggccgttgag ccagtcgaca accgccttga ccgagaggat
      421 gtcggaggcg gctccttcgt cgacgcagcc tgtcgaacgg ttggtgccgg ccatgtcgac
      481 ggcggcgtag gcgtagccgc gtgggacgta gtagttgtcg tagaagagcg ggaacttcgt
      541 cgggttgccg tcggcgtcgt actccttgag ctcgctctcg ttcccacgtc cacagcaggc
      601 gtagtaggga ctcgcgtcca tgatcaccgg gatctgcgcc gtgccgtcga gctcggcagg
      661 ccggatgatg tcgacggcga cgcggtcggc ctgcccgttg tggtcgaggt cgggcgcgag
      721 gacccacacc gactggcgga tggcgtcggc gtaggagtag acgggcgtgg tctcgccgtt
      781 cctcaactgg tacgggtggg tccgcgttgc ggccgtggat gcggtcgtgg gtcctgtcgt
      841 tcctgccgtg gcagagactg cgttggcggc ggtcagggcg gtggtggggg ctacgagtgc
      901 gagtgtcacg agagccgtga aaacgactcg tcgggtgcgc gacgatgtca ttttggtgac
      961 tatgccacca acccggacga ggcgaaagcc gaccgtggaa tacccgatga gcacctgcgc
     1021 gaatgccgac cgaggtggca atcgcgtgcc gacacaccgg cagaactcaa cgaaagttgc
     1081 cacctcggcg gtggccgcct cggcagggac cagctcggcg tcaggggcgg cggctcgcaa
     1141 ggacgcagaa ctcgttcccc tccgggtcgg caagcacgac ccagctcacc tcgccttggc
     1201 cgacgtcggc gtgtcgagca cccaggtcga gcaggcgacg gacctcttcg tcctgctccc
     1261 ggtcggtcgg gttgacatcg aggtgcagcc ggttcttgac ggtcttgcgc tcagtcacgc
     1321 gcgcgaatgt cagcgtcggt ggtaccggac cagggcggtt cttcccctcc ggcaccatgg
     1381 gggagccgat cgtgacaact ccggcgtcct cgtcctgcac ctcgtagtcg aggaccgagc
     1441 accagaaccg ggcgagaccg ttggggtcgg cacagtcgat cgcaagctcg gtgaacttgc
     1501 tggtcatgtc aggacctccc tggcagcgac gggtctgctc accccagcca tccttcccga
     1561 ctcactccac tccgtcgtcg ttcgaacgtg tgttcgaact cgtgataacc tgccgacgtg
     1621 actgcctacg cgccgccggg gtggccggag caggttcgcc ccccgggcgc gccgtggtgg
     1681 gaggacaccg ccgtcggctg gctgctcgac cagtgcccgc ccgactaccg gatgtatcag
     1741 gggctgcggc gtcacccggt cgtgctggcc cggttcgcgg cgctgcacac cgaggcgagc
     1801 atccaagcgg cccgacgcgg catctccgag gcgcgcacga gcctgcgcga cgtgcttccc
     1861 accgagtcgg tcgagggcgc gatcgcgatg tgggaacggg agggggcacg cctgctccgg
     1921 gtacggcgag ccgtcggtct catcgaggag gcgtttcgcg gccggcagtt ccgggccaag
     1981 ctctgatctc acgcttctcg ccgacgtggc aactttcgtt gagttctgcc ggcgtgtcgg
     2041 tccacgatcg acacctcgtc ggcgtacgtg atgctgacga cagactccgc cagggcatcg
     2101 ccccacaacc agccgcaggc gcctacactc gacgggacga cctgcgcctc gccgtgcagg
     2161 agccacggca ccacagctcg acgaaggggc cttgatgggc atgctcgacc gcatcgaggg
     2221 accgagtgac ctgcgcgacc tcgacgagca gcagctgcgc gaactcgccg acgagatccg
     2281 cacgttcctc atcacgaccg tgtcgaagac cggcggccat ctgggcccca acctcggcgt
     2341 cgtcgagctg acgctggcgc tgcaccgcac cttcgactcg ccgcatgacc gcatcgtctt
     2401 cgacaccggt caccaggcgt atgtccacaa gatcctgacc ggtcgcgccg accagttcga
     2461 gacgctgcga cagcaaggcg gtctctccgg ttatccgcgg cggtcggagt ccgagcacga
     2521 catcgtcgag aaccagcacg cttcgacgtc gctgtcgtac ggcgccgggc tggctgcggc
     2581 gtactccatg cagggtgacg accgatacgt cctcacagtg atcggcgacg gagcgctcac
     2641 cggcggcatg gcatgggagg cgctcaacaa catcgcggcc gccaagcact cgcggctggt
     2701 catcgtggtc aacgacaacg gtcgttccta cagcccgacg gtcggtggac tggccgagca
     2761 cctggccgga ctgcgcaccg acccgcgcta cgagcaggtg ctcgagcgga tcaagcgccg
     2821 tctcaaccgc acgaagctcg tcggacctcc gttgtacgac gccttgcatg cgatcaagaa
     2881 gggcctcaag gacgcgctcg ccccgcaagg catgttcgag gacctcggcc tgaagtacgt
     2941 cggcccggtc gacggccacg accgcgcgac gctcgagcaa gcgttggacc aggccaagaa
     3001 gttcgccggg ccggtgctcg tgcacgcgat cacccgcaag gggaacggct atccgccggc
     3061 cgagcgccac gacgaggaca acttccacac gctgagtgcg ttcgacccga gcacgggcga
     3121 ggtcatcgcg cagtccccga ccatctggac cgacatgttc agcgacgaac tggtccggct
     3181 cggtgaggag cgcaaggaca tcgtggcgat cacggccgcg atgctgcacc cgaccggact
     3241 gcatgccttc gccgcgaagt tccccgaccg aacgttcgac gtcgggatcg ccgaacagca
     3301 cgccgtgacg tcggcgaccg gcatggcgat ggccggtctg cacccggtgg tcgcgatcta
     3361 cgcgacgttc gccaaccgtg cgttcgacca ggtgttgatg gacgcggccc tgcatggttg
     3421 cggcgtcacc ctcgtcctcg accgcgccgg tgtcacaggc gaggacgggg cgtcccacaa
     3481 cggcatgtgg gacatgtcga tcttccaggt cgtgccgggc ctgcggatcg cggcgccccg
     3541 tgacggcacc cgcgtccgcg agctgttacg ggaggcggtc gcggtcggcg atgcacccac
     3601 cgtgctccgt atcccgaagg gtcaggtcca gtccgacatc gcggcgatcg agaccgtcga
     3661 cggcgtcgac gtgctgtacc gccagggcga gcgcgacgtg ctggtcgtcg ccgcgggcgc
     3721 gatggccgcg acctgcctgg acgttgcggc gcgactgcgc gcgcagggca tcggtgtgac
     3781 cgtggtcgac ccgcggtggg tgaagccggt caacccgcgg ctggtgacga tggcgcgtga
     3841 gcaccggctc gtggtttccg tcgaggacaa cgggcgggtc ggcggctgcg gcgcggtgct
     3901 gaggcaggtg ttggcggacg ccgaggtcga cgtgccgacc atggtgttcg gcatcccgca
     3961 ggagttcctc gaccacgcca agcggtcggt catcctggag gcggtgggtc tcggcgcgca
     4021 gagcctgtcg cgcgacatca ccgcgcgggt cgccggcctg gaccggcacg agtcgcccag
     4081 ccatacccgg gcggacacgt cggccgaccc cggctgatct gcacgcccgc gcgctccgtt
     4141 ctagggtgag gtaacgcagc acccgccggg aggagccccg tgatgagcgt gttccgcacc
     4201 aagagcatcg agcagtcgct gaaggattcg gaggatcctg agttccagct caaacggtct
     4261 ctgacggcaa tcgacctcac ggtcttcggg atcggcgtga tcatcggcgc cggtatcttc
     4321 accctcaccg gtcaggtcgc cgccgagacg gcagggccgg cggtgtcgat cgcgttcgtg
     4381 atctccgcgg tctgttgtgc gttcgccgcg ctctgctatg cggagttcgc ctccgccgta
     4441 ccggtgtccg gttcggcgta cacgttctcc tacgccacgg tcggcgaact ggtcgcatgg
     4501 atcatcggct gggacctgct gctcgagctg atgctcggtg cttctgtcgt cgcacagggg
     4561 tggagtcagt acgccgagca gctgctcgat gagatcggtc tcggctggcc cgactccgtc
     4621 ggtccgggat cgcacttcaa cctgctcgcg ttcctgctcg tggcggtgtt gtgcgtcctg
     4681 gtcacgatcg ggatcaagga gtcgatgcgc gtcaacatgg tgctggtcac gatcaaggtc
     4741 ttcatcgtgc tgttcgtcat cgtcatgggc atctccttga tcaacacgtc gaactacacg
     4801 ccgttcgttc ctgacaagat ccccgccacc gacaacagcg tcgacctcac gacgacggtg
     4861 tggcaggcgc tgaccggcgc acccggccag tacggctgga ccggcgtgtt cgccgccgcg
     4921 tcgatcgtgt tcttcgcgtt catcggcttc gacgtggtgg cgaccaccgc ggaggaggcg
     4981 gagaacccgc agcgcgacct gcccatcggg atcatcgcgt cgctggccat ctgtaccgtc
     5041 ttgtacgtcg ccgtcgcgtt ggtgatcacc gggatggtgg actacccgaa catcgatccc
     5101 gaggcggctc ttgcgtcggc gttcagcgac cggggacacg gcacggtggc gacgatcgtg
     5161 tccgccggcg ctgtcgcggg gctcaccacg gtcgtgatga cgttgctcat cggcgccacc
     5221 agggtgacgt tcgcgatgag ccgcgaccac ctgctcccgc gcgagttcgg ccacgtgaac
     5281 cgcaccacca ggacgccgat gatcatcacg atcggtatcg gcgtcatcgt cgcggcgacc
     5341 gcgtcgctga ctccgatcga caagctcgag gagatggtca acatcgggac gctggcggcg
     5401 ttcacgctcg tgtcgatcgc cgtggtgatc ctgcggcgta cccggccaga tctcgaccgt
     5461 ccgttcaagg tgcccggtgc gccgttcacc ccgatcctgt cagccgtgat ctgcgtctac
     5521 atgatgctca acctgacgat cgagacgtgg ctgcggttcg cgatctggat gctgatcggc
     5581 ttcgtgatct acttcggtta cggcatacgc aagagtcggg tcggcgaggc ggagagagcg
     5641 aagagcgctc gccgacagcc cgcctgacag
//
LOCUS       DATBFZ010000457        10204 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_3768403, whole genome shotgun sequence.
ACCESSION   DATBFZ010000457 DATBFZ010000000
VERSION     DATBFZ010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 10204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10204
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3768403"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..742
                     /locus_tag="VH419_13910"
     CDS             <1..742
                     /locus_tag="VH419_13910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044760.1"
                     /translation="SEPTLRHLHRFIDRMAKSVRVVVYLRRQDDHMVSRYQQGVKIGW
                     IRRLCDWAHEDMTALYDYAARLDRHRELLAPTEFVVRRFERSCFADGSLYQDFLDAAG
                     IDARVDDLEQVADRNVSFDAESVEFLRLLNLHRVENEGATEGLIDNRRFVTRLAGSSN
                     GPVLTLPPPFLDEFMVQWEESNRRVARQFLGDKSGQLFHLPRKSDNTTAEQRLDPRRI
                     DHFVSLLDLPDSIHAPLRALAEREAKAR"
     gene            complement(773..1537)
                     /locus_tag="VH419_13915"
     CDS             complement(773..1537)
                     /locus_tag="VH419_13915"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HEY7044761.1"
                     /translation="MTSDLTVIMTAHDETAVCGPTMRAADLAIEAARGRGITVHAVIG
                     LDAPTAGTLTYFEQSHFDHWERRVLGEREVGTARNALVRECDSRYIAFLDADDLVSEN
                     WLAEGVAALDAAAARGEQAIAHPELNVIFDGAKEVLLNIDPGSPLFTPHYLYFRHYYD
                     SMCMAPRQAHVDMPYVTRDVVNGLSYQDFQFTIESLAAGWRHIVVRDTIIFKRRRDVS
                     LVTESNTRKSVVRALPAMAIDRIRDLGAAQRPNVRP"
     gene            complement(1534..3393)
                     /locus_tag="VH419_13920"
     CDS             complement(1534..3393)
                     /locus_tag="VH419_13920"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY7044762.1"
                     /translation="MTADGSAGKFRDQFRRGGGAAKRRFRRPVESVDVPPGPQGPPRE
                     AVDDQSHVGPIMDAAVARVRGAMRPAGLDRDYDLAYEHFDLMHFLLQARFLLSAGVDP
                     LQHFLDNGARAKASPEINFSMRSYVARYPEKKRAGRSPYLDWLEAGKEAGQIADPAPG
                     LEQMALILGMEPSELVTTLAETRLDLQARLRTGTLGAMFAKAAEVEPLVADSWPETTR
                     PTIPPLYSANTVDQVAAIHRCQQVAGFARARLVLVASNPRWGGGRRMEGHIAHALTGR
                     LDAREIVVIYTDKGGTAPPGRFPQRVREVDFATEVDGMDPLAAQRALVELIRSFHAEA
                     VVIINSRLLYEAMTTYGRALAASERLFLMLFCNEQMASGNWVGYPLRFFYRCFDVVEG
                     VITDSDYRANWLRHRHQLGPAEASRIHVLHAPVDPAMPLVSAPTSDPSRRRQVFWAGR
                     WDRQKRIDIALDVARRMPELDFRLWGESVLTRAHVDEASDNVRLEGAYAHISDLRLSD
                     ADVWLYTSAWDGVPSQLLEVAMTGIPIVGSLVGGTGEVLSLEHAWPVADLEDPEAYVR
                     AIRDVLADPPGARRRARALRDRLLVERTDSAFADHVAGLLLRDDPAAGKQVDG"
     gene            complement(3439..4392)
                     /locus_tag="VH419_13925"
     CDS             complement(3439..4392)
                     /locus_tag="VH419_13925"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TylF/MycF/NovP-related O-methyltransferase"
                     /protein_id="HEY7044763.1"
                     /translation="MWKQTVNDVLRRLTGYQVQRAHTVDQMARQRRARQREVMRLKSQ
                     LDLANTRLARTNVRLDETTARLDQAKRRLDKARARARQQRHAVKKARGMPKHYDDAAV
                     EILRRVKPRTMTEHGKLFGLVEATRYVVRRGIPGDIVECGVWRGGSMQAVALTLMEQG
                     VTDRRLHLFDTFEGMPPPSEKDRRPQGASAEELLATHDKHHRIWAIAGLDDVQEGMRE
                     TEYPEEQIHFHKGLVEDTVPVHAPAQLAILRLDTDWYESTRHELEHLFHRLSPGGVLI
                     IDDYGDWDGARQATDEFMDKLAEPLLLLPVGSGRLAVKPYV"
     gene            complement(4473..5432)
                     /locus_tag="VH419_13930"
     CDS             complement(4473..5432)
                     /locus_tag="VH419_13930"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY7044764.1"
                     /translation="MRIKRILACLFAAATLFAVAGCGGDDNSLEDQGGSSDGGAGDKG
                     SVTVGGQDFTESQVMAAIYQALLENEGFDVTTKLVQTRDIYLPELSKGNVDIVPDYLS
                     GITDFLNTEKNGPDAPLVSSHSPEKTLAALKPLAEAKGISVLAPSDATDQNAFFVTTD
                     FADENNLRTLSDLAALGQPIKLAANSDCPGRADCLDGLKNVYGLDISKFEPLEFGSAQ
                     MKDAVKSGEVDLGQTGTTDGTLTDAGFTLLQDDKGIQPAQNLTPVVNSDFLDANPDLE
                     ALFDKLSQTLSTQDLAEMNAKVDVERQKPEDVANEFLTENGLI"
     gene            complement(5607..6314)
                     /locus_tag="VH419_13935"
     CDS             complement(5607..6314)
                     /locus_tag="VH419_13935"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY7044765.1"
                     /translation="MLSEVWSYLSDGANWSGSEGIGAYFVQQLLLTVTSLAIAMVLGL
                     PIALYLGHRGRGGFVAVNVSNVGRAIPVFAVLIVLSIGPVGAAEFGPYGRAGLATLIA
                     LVMFALPPLITNAYVGVREVDRDVVEASRGMGMGGADVFRRIELPLAMPVIFTGIRLA
                     LVQIWATATIAALVAGPGLGRIITDGFANQRTYQVVAGAVLVAVGALVLEGLAWLAER
                     SVDPMRHVRRGRAARHG"
     gene            complement(6316..7098)
                     /locus_tag="VH419_13940"
     CDS             complement(6316..7098)
                     /locus_tag="VH419_13940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011753946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY7044766.1"
                     /translation="MTLLAALAAHLTSRAADPSPIQGGGTAHHATVRAADSIPQCYVD
                     NDWICAYYWKDRRTELIDATQQHLSITVISVLLGFAVALPLGLVARRYRNLESLIIGG
                     TTIVYTIPSLALFSLLLPWTGLTETTVVIGLALYSLTILVRNVLAGLRSVPDEVRESA
                     LGLGYGPARLLFKVELPLALPVIMAGVRVATVSTVALVTIGAIVAYGGLGNLLYEAFP
                     SEFKAQIFAASLLCIVLAVVLDIVLVVAQRLMTPWTRVGRSS"
     gene            7102..8295
                     /locus_tag="VH419_13945"
     CDS             7102..8295
                     /locus_tag="VH419_13945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015071709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY7044767.1"
                     /translation="MDATERRDGWPMIRLQGVGKTYADGTVAVRELDLDVPGGELVIL
                     VGPSGCGKSTTLKMINRLIEPTTGRIELDGQDVTSMNPVLLRRRMGYVIQQIGLFPHQ
                     TIRANVATLPRLLGWDRKRTNARVDELLETMGLDVATFAARYPHQLSGGQRQRVGVAR
                     ALAADPDVLLMDEPFGAVDPIVRTRLQDQFLEIQETLAKTVVLVTHDIEEAVRLGDRV
                     AVFEPGGRLAQFDTPARVLGDPGDEFVAEFVGADRGLRRLAVTPIDQIDLERPPIVHD
                     TDTLAEAHLSMDAEDARWAVVLDKNDNLRGWIGADDCEGAGTVADRSDRMDAWVDKDA
                     SLKDALATMLQWDAGWVAVLEGDQFLGVLTPNTLHEALRRSVGAEHDGVDLDEVSVST
                     TGPLH"
     gene            complement(8313..8933)
                     /locus_tag="VH419_13950"
     CDS             complement(8313..8933)
                     /locus_tag="VH419_13950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044768.1"
                     /translation="MLAGRSGMRRGSRRVQRSVRLVVATILLTLATVLVATAVATASL
                     VGVAAAASLLAGIVSCRIVSTEVTQTRRDAARQRAELAREFGAAMSRARHQHSVFTAT
                     MASRLAERDTTIVELYANLRLAERRAADAEIRVKREARRANEAQQRLSQLLDEVLLRQ
                     SDDAAADAEPTGPGIPEAADLPTVVELLVWEQRVSDATAQDLRRHA"
     gene            complement(8943..9449)
                     /locus_tag="VH419_13955"
     CDS             complement(8943..9449)
                     /locus_tag="VH419_13955"
                     /inference="COORDINATES: protein motif:HMM:NF022819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3180 domain-containing protein"
                     /protein_id="HEY7044769.1"
                     /translation="MAPSFRPTRPEKDPPQGTIVPTSLRLLALTFVVGGLLGYALAVI
                     SEQVSSTSSAPRVQWPAVVALAAIAVANVVLAWWTYWSVHRERRRIDPHLAVTFLLFA
                     KASSVVGAFVAGGYAGFALHFVDDMEADLPRERVIRSIVAAVTGVAIVISALLLERAC
                     RVPKQEDE"
     gene            complement(9464..10027)
                     /gene="folK"
                     /locus_tag="VH419_13960"
     CDS             complement(9464..10027)
                     /gene="folK"
                     /locus_tag="VH419_13960"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008354939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEY7044770.1"
                     /translation="MTETPNPHIIDPDTLTGEMRPIRPAVISLGSNVGDRLANLQGAV
                     DALADTPEVHLVTVSAVYETAPVDAPDGSPDFLNAIVLADTTLSVTTLLDRAQAIEAA
                     FGRERAEFNAPRTLDVDLIVVGDRLSDTEELVLPHPRAHDRAFVLVPWQDVDEQATIP
                     GKGKIADLLSDLDTSGVVRRDDLELEY"
     gene            complement(10020..>10204)
                     /locus_tag="VH419_13965"
     CDS             complement(10020..>10204)
                     /locus_tag="VH419_13965"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011753940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEY7044771.1"
                     /translation="HSSLTSDPVDLIETLAQRLAEICLAEPPVSWVSVTVHKPHAPIA
                     VPFDDVTLTIHRSRDD"
BASE COUNT         1698 a         3392 c         3455 g         1659 t
ORIGIN      
        1 ctccgaaccg acactgcgcc acctgcaccg gttcatagat cgaatggcaa agagcgtgcg
       61 tgtcgtcgtg tacttgcgtc gtcaggacga ccacatggtc agccgctacc agcagggcgt
      121 gaagatcggt tggatccggc ggctgtgcga ctgggcccac gaggacatga ccgccctcta
      181 cgactatgcc gctcgtctcg accggcaccg agaactgttg gcccccaccg agttcgtcgt
      241 acgacgcttc gagcgcagct gcttcgccga tggatcgctg taccaggact tcctcgacgc
      301 ggcggggatc gacgcccggg tcgatgacct ggagcaggtc gccgaccgca acgtgagctt
      361 cgacgccgag agcgtggagt tcctccggtt gctgaacctc caccgggtcg agaacgaagg
      421 cgccaccgag ggcctgatcg acaatcggag atttgtcacc cgcctggccg ggtcttccaa
      481 cggaccggtc ctgaccttgc ctcccccgtt ccttgacgag ttcatggtgc aatgggagga
      541 gtccaaccga cgtgtggcga ggcagttcct gggcgacaag agtggtcagc tgttccacct
      601 gccgaggaag agcgacaaca ccacggccga gcagcggctc gaccctcgtc ggatcgatca
      661 tttcgtcagc ttgttggacc tcccggattc gatacacgct ccgttgcgcg cgctcgccga
      721 acgcgaggcg aaggcacgat gacgggggca cgccgcgagc tgggccttcg ccctagggtc
      781 gaacgttcgg gcgttgcgcc gcccccaggt cgcggatccg atcgatggcc atcgccggta
      841 gcgctcgcac gaccgacttg cgtgtgttgc tctcggtgac gagcgaaacg tcgcggcggc
      901 gcttgaagat gatcgtgtct ctcaccacga tgtgccgcca gccggcggcg agactctcga
      961 tggtgaactg gaagtcctga tacgacaggc cattcacgac atcccttgtc acgtagggca
     1021 tgtcaacgtg cgcctgccgc ggtgccatgc acatcgagtc gtagtagtgc cggaagtaca
     1081 ggtagtgcgg cgtgaacagc ggcgagcccg ggtcgatgtt gagcaatacc tccttcgccc
     1141 cgtcgaagat cacgttgagc tccggatggg cgatggcctg ctcgccgcgc gcggcggccg
     1201 cgtccagggc agccaccccc tcagccaacc agttctcgct gaccaggtcg tcggcgtcca
     1261 gaaaggcgat gtaccgactg tcgcactcgc ggacgagcgc gtttcgtgcc gtgcctactt
     1321 cacgctcgcc caggactcgc cgctcccaat ggtcgaagtg cgactgctcg aagtacgtca
     1381 aggtcccggc cgtcggcgcg tcgagtccga tgaccgcatg gaccgtgatc ccgcggccac
     1441 gagccgcttc gatcgccagg tctgccgcgc gcatcgtagg tccgcagacg gccgtctcgt
     1501 cgtgggcggt catgatcacc gtgagatcgc tcgtcatccg tcgacctgct tcccggcggc
     1561 cggatcgtca cggaggagca ggccggcgac gtggtcggcg aaagcggagt cggtgcgttc
     1621 cacgagcagc cggtctcgca gcgcacgtgc gcgccgcctc gccccaggcg ggtcggcgag
     1681 cacgtcgcgg atggctcgca cgtacgcttc cgggtcctcg aggtctgcca ccggccatgc
     1741 gtgctcgagg ctgagcacct cacccgtacc cccgacgagg ctgccgacga tcggaatgcc
     1801 ggtcatagcc acctccagga gctggctcgg aaccccgtcc caggcggacg tgtagagcca
     1861 gacatcggcg tcggacaggc gaagatccga gatgtgtgcg taggcgccct ccagtcggac
     1921 gttgtcagat gcctcgtcga catgggcacg ggtcagcacc gactcccccc acagccggaa
     1981 gtccagctcg ggcatgcgtc gggcgacgtc gagcgcgatg tcgatgcgct tctgccgatc
     2041 ccaccgaccc gcccagaaca cctgccgccg ccgcgagggg tcggacgtag gcgccgaaac
     2101 caggggcatg gcaggatcta ccggcgcgtg caggacatgg atgcggctcg cctcggccgg
     2161 acccagctga tgcctgtgac gcagccagtt cgcccggtaa tcgctgtcgg tgatgacccc
     2221 ttcgacgacg tcgaagcacc ggtagaagaa ccgcaacggg tagcccaccc agttgcccga
     2281 cgccatctgc tcgttgcaga acaacatcaa gaagagacgc tcggacgcag ccaaggcccg
     2341 gccgtacgtc gtcatggcct cgtagagcag ccgcgagttg atgatgacga cggcctcggc
     2401 gtggaacgag cggatcagct cgacgagcgc ccgctgagca gcgagcgggt ccataccgtc
     2461 cacctcggtg gcgaagtcga cttcccgcac ccgctggggg aatcgacctg gcggcgcggt
     2521 cccgcccttg tcggtgtaga tcaccacgat ctcgcgagcg tcgagtcgac cggtcagggc
     2581 atgtgcgatg tgcccctcca tccgcctgcc acctccccag cgggggttgg aagccaccag
     2641 cacgagacgt gcgcgggcga acccggccac ctgctggcag cggtggatgg cggcgacctg
     2701 gtcgacggtg ttcgcggagt acagcggcgg gatggtcggt ctggttgtct ccggccacga
     2761 gtcggccacc aatggctcga cctcggctgc cttggcgaac atcgcgccga gggttcctgt
     2821 ccgcagccgt gcctgcagat ccagacgtgt ttcggcaagt gttgtcacaa gctccgatgg
     2881 ctccatgccg aggatgagcg ccatctgctc caggccagga gccggatcgg cgatctgacc
     2941 ggcctccttc ccggcctcca gccagtccag gtacgggctg cgaccggcac gtttcttctc
     3001 gggataccgg gcgacgtagg accgcatgct gaagttgatc tcggggctgg ccttggctct
     3061 cgcgccgttg tccaggaagt gctgcagcgg atcgaccccg gcggacagca ggaaccgcgc
     3121 ctgcagcagg aagtgcatga ggtcgaagtg ctcgtatgcg aggtcgtagt cgcggtcgag
     3181 gcccgccgga cgcatcgctc cacgcacacg cgcgaccgca gcgtccatga tcgggccgac
     3241 gtgggactga tcgtcaactg cctctcgagg aggtccttgc ggaccagggg gaacgtcgac
     3301 gctctcgacc ggccgccgaa accgacgctt ggccgcgccg cctccccggc ggaattggtc
     3361 gcggaacttc ccggccgatc cgtcagcggt caccgaggag ccttccactc gtttcgtgtc
     3421 atctcgacgc tgtgtgcatc agacataggg cttgaccgca aggcggccgc tccctacggg
     3481 aagcagcagc aggggttcgg ccaacttgtc catgaactcg tcggtcgcct gtcgagcgcc
     3541 gtcccagtcg ccgtagtcgt cgatgatcag cacaccgccg ggtgagaggc ggtggaagag
     3601 atgctcgagc tcgtgtcgcg tcgactcgta ccagtccgtg tccaggcgga ggatcgccag
     3661 ctgagccggc gcatgcactg ggacggtgtc ttcgaccagg cccttgtgga agtgaatctg
     3721 ttcctccgga tactcggtct cgcgcatgcc ctcctgtacg tcgtcgaggc ccgcgatcgc
     3781 ccagatgcgg tggtgcttgt cgtgggtggc caacagctcc tctgcactgg ccccctgcgg
     3841 gcgtcggtcc ttctcggaag gtggcggcat cccttcgaag gtgtcgaaca ggtggagccg
     3901 acggtcggtc acgccttgct ccatcagcgt cagtgcgacg gcctgcatgc tgcccccgcg
     3961 ccagacaccg cattcgacga tgtctccggg gattccgcgc ctgacaacgt acctcgtcgc
     4021 ctcgacgagt ccgaagagct tgccatgctc ggtcatggtt cgaggcttca cccgacgcag
     4081 gatctccacg gctgcatcgt cgtagtgttt cggcataccc cgggccttct tcaccgcgtg
     4141 ccgttgttgc ctggctctgg cgcgagcctt gtcgagtcgg cgtttcgcct ggtcgagtcg
     4201 tgccgtcgtc tcatcgaggc gcacgttggt ccgagcgagc cgtgtgttgg ccaggtcgag
     4261 ctgactcttc aaccgcatga cttctcgctg acgggcccgt cgctgtctcg ccatctggtc
     4321 gaccgtgtgg gcacgttgaa cttggtagcc ggtgagccga cgcagcacgt cgttgactgt
     4381 ctgcttccac atcgaggcca accctaacgg ccggccaccg gcacctgcgg tcgggccgga
     4441 cggcaacgcc ggggctcgac gatcggctcg gctcagatga ggccgttctc cgtcaggaac
     4501 tcattggcga cgtcttccgg cttctgccgc tcgacgtcga ccttcgcgtt catctcggcc
     4561 agatcctgcg tcgacaacgt ctgcgacagc ttgtcgaaga gcgcctcgag gtcagggttg
     4621 gcgtcgagga agtcgctgtt gaccaccggc gtgaggttct gcgcgggctg gatgcccttg
     4681 tcgtcctgca gcagagtgaa tcccgcatcg gtgagagtgc cgtccgtcgt acccgtctgg
     4741 cccaggtcga cctcgccgct cttcacggcg tccttcatct gggcactgcc gaactccagc
     4801 ggctcgaact tggagatgtc gagcccgtag acgttcttca gcccgtcgag acagtccgca
     4861 cggccagggc agtccgagtt cgccgcgagc ttgatgggct ggccgagcgc ggcgaggtcc
     4921 gacagcgtcc gcaggttgtt ctcgtcggcg aagtccgtgg tgacgaagaa ggcgttctgg
     4981 tcggttgcat cggagggagc cagcaccgat atccctttcg cctccgccag cggcttcaag
     5041 gcggcgagcg tcttctccgg cgagtggctg gacaccaacg gcgcgtcggg gccgttcttc
     5101 tcggtgttga ggaagtcggt gatcccggaa aggtagtcgg gcacgatgtc gacgttgccc
     5161 ttgctgagct cgggaaggta gatgtcgcgg gtctggacga gcttggtcgt cacatcgaag
     5221 ccttcgttct cgagcagcgc ctgatagatc gcggccatga cctggctctc ggtgaagtcc
     5281 tgaccgccga cggtcacgct gcccttgtcc ccggcgcccc cgtcgctgct accgccttgg
     5341 tcctccagcg agttgtcgtc gccgccgcag cccgcgaccg cgaacagcgt tgcagcagcg
     5401 aacaggcagg caaggatgcg cttgatccgc atgggtgaac caccttctgt gtcccgcgag
     5461 cgagccgctc gcgcgtgtcc gatgggcacc cgatgtcggc acccgcccta acggaatcac
     5521 ccaacgccga cagtggcaag ccaccaactg ctcacaaacg ggtcacgatg accccgtcca
     5581 cgccgtcgga aagcttgtcc aggtgctcaa ccatgacgtg ccgcgcggcc gcgtcggacg
     5641 tgtcgcatgg gatcgacaga ccgctcggcc agccaggcaa gcccctcgag gaccaacgcg
     5701 ccgacggcca ccaacacggc gccggcgacg acctggtagg tgcgctggtt ggcgaagccg
     5761 tcggtgatga tccggccgag tcccggcccg gcgaccagtg ctgcgatggt ggcggtggcc
     5821 cagatctgga ccagcgcgag cctgatgccg gtgaagatca ccggcatcgc cagcgggagc
     5881 tcgatccgcc ggaagacgtc ggcgccgccc atgcccatgc cacgcgacgc ctccacgacg
     5941 tccctgtcca cctcacgtac gccgacgtac gcgttggtga tgagcggcgg cagcgcgaac
     6001 atgaccaacg cgatcagcgt cgcgagaccg gcccgcccgt acggaccgaa ctcggctgct
     6061 ccgaccgggc cgatcgacaa cacgatcagc accgcgaaca cgggaattgc tcggccgacg
     6121 ttggagacgt tcactgcgac gaagccgccg cggccccggt gccccaggta cagggcgatc
     6181 gggaggccga gcaccatggc aatggcgaga ctggtgacgg tgaggaggag ctgctggacg
     6241 aagtatgcgc cgatgccctc cgagcccgac cagttggctc cgtcggacag gtacgaccag
     6301 acctcggaga gcatgtcagg agctccgtcc gacccgggtc cacggcgtca tgaggcgttg
     6361 cgcgacgacg aggacgatgt cgaggacgac ggccagcacg atgcacaaca gacttgctgc
     6421 gaagatctgc gccttgaact cactggggaa cgcttcgtac agcaggttgc ccagcccgcc
     6481 gtacgcgacg atcgctccga tggtgacgag tgccaccgtg gaaaccgtgg cgacccgaac
     6541 cccggccatg atgacgggta gcgcgagcgg cagctccacc ttgaacagca gccttgccgg
     6601 cccgtagccc agtcctagcg cggactcgcg gacctcgtcc ggaacggacc ggaggccggc
     6661 gagtacgttg cggacgagaa tcgtcagcga gtacagggcg agaccgatca ccacagtggt
     6721 ctcggtgagt ccggtccacg gcagcagcag cgagaacagc gccagcgacg ggatcgtgta
     6781 gacgatcgtg gtgccgccga tgatgagcga ctcgaggttc cggtagcggc gcgccaccag
     6841 acccaacggc agcgccaccg cgaaccccag caagaccgag atcacggtga tcgacaggtg
     6901 ctgctgggtc gcgtcgatca gctcggtacg ccggtccttc cagtagtagg cgcagatcca
     6961 gtcgttgtcg acgtagcact gcgggatcga gtcggccgca cgaacagtgg cgtgatgcgc
     7021 cgtccctccc ccctggatcg ggctgggatc ggcggctctg ctggtcaggt gcgccgccag
     7081 ggcggccagt agagtcacgg aatggacgct accgaacgtc gcgacggttg gccgatgatc
     7141 cggctgcagg gcgtcggcaa gacgtacgcc gacgggacgg tggccgtccg agagctcgac
     7201 ctcgacgtac cggggggcga gcttgtcata ctcgtcggac cgtcggggtg cgggaagtcg
     7261 acgacgctga agatgatcaa ccggttgatc gagccgacga cgggccggat cgagctcgac
     7321 ggccaggacg tcaccagcat gaacccggtc ctgctgcggc gacggatggg ttacgtgatc
     7381 cagcagatcg ggctgttccc gcaccagacg atccgcgcca atgtcgcgac cctgccccgc
     7441 ctgctcggct gggaccggaa gcgcacgaac gcccgcgtcg acgagctgct ggagacgatg
     7501 gggctggacg tggccacctt cgccgcccgc tacccgcacc agctgtcggg tgggcagcgg
     7561 caacgcgtcg gcgtagcccg tgccctggca gcggacccgg acgtgctgct gatggacgag
     7621 ccgttcggcg ccgtcgaccc gatcgtacgg acccgcctcc aggaccagtt cctggagatc
     7681 caggagacgt tggccaagac cgtggtcctg gtgacgcacg acatcgagga ggcggtccgg
     7741 ctcggcgacc gcgtcgcggt cttcgagccg ggcggccggc tcgcgcagtt cgacacccct
     7801 gcacgcgtcc ttggcgaccc cggcgacgag ttcgtggccg agttcgtggg tgccgaccgt
     7861 ggactgcgcc gactggccgt caccccgatc gatcagatcg acctggagcg tccgccgatc
     7921 gtgcacgaca ccgacacact ggccgaggcc cacttgtcga tggatgccga ggacgcgcgg
     7981 tgggcggtgg tgctggacaa gaacgacaac ctacgtggct ggatcggcgc ggacgactgc
     8041 gagggcgcgg ggacggttgc cgaccgctcc gaccggatgg acgcctgggt cgacaaggac
     8101 gcgtcgctga aggatgcgct cgccacgatg ctgcagtggg acgccggctg ggtggcggtg
     8161 ctcgagggtg atcagttcct cggcgtactg acgcccaaca cactgcacga ggcgttgcgt
     8221 cgttccgtgg gcgccgagca cgacggggtt gacctcgacg aggtcagcgt ctccaccacc
     8281 ggccccctcc actgagcagg gcgtacgacg ggtcaggcgt ggcggcgcag gtcttgggcc
     8341 gtggcgtcgc tgacccgttg ctcccagaca agcagctcga cgaccgtggg gaggtcggcc
     8401 gcctccggaa tccctgggcc agtcggctca gcatcagctg ccgcgtcgtc ggactggcgc
     8461 agcagcacct cgtcgagcag ctgtgacaac cgctgctgag cctcgttggc gcgcctggct
     8521 tccctcttga cgcggatctc cgcgtccgcg gcacgtcgct cggccagccg gaggttcgcg
     8581 tacagctcga cgatggtggt gtcacgctcg gcgagccgcg atgccatcgt ggcggtgaac
     8641 acggagtgct ggtgacgggc tctgctcatc gcggcgccga actcccgcgc cagctcggca
     8701 cgctgcctcg cggcgtctcg ccgggtctgg gtgacctcgg tggagacgat ccggcacgag
     8761 acgatcccgg ccagcagact cgccgcggcg gccacgccga ccaacgacgc ggtcgccacc
     8821 gcagtggcga cgagaacagt ggcaagcgtc agcaggatcg tcgcgacgac gagccggacg
     8881 ctgcgctgga cccggcgcga gccacggcgc attccggacc tgcctgccag catgtcggca
     8941 gcctattcgt cctcctgctt gggaactcgg caggcacgct cgagcagcag cgcagaaatc
     9001 acaatcgcca cacctgtcac agccgccaca atggatcgta tgaccctttc gcgcggaaga
     9061 tccgcttcca tgtcgtcgac gaagtggagc gcgaaaccgg cgtacccgcc ggccacgaag
     9121 gcaccgacga ccgagctcgc cttcgcgaac agcaggaacg tcaccgccag gtgcgggtcg
     9181 atccgccgtc gctcgcgatg cacgctccag tacgtccacc acgccagcac gacgttcgcc
     9241 accgcgatcg ccgccaacgc cacgaccgcc ggccactgga ctcgcggcgc cgacgacgtc
     9301 gagctcacct gctcggagat gacagccagg gcataaccca gcaacccgcc gaccacgaag
     9361 gtgagcgcga gcagccgcag cgaggtgggg acgatcgttc cctgcggcgg gtccttctca
     9421 gggcgtgtcg gccgaaacga gggtgccaag ggagttgcgg gactcagtac tcgagctcga
     9481 ggtcgtcacg gcgtacgacg cctgacgtgt cgaggtcgga cagcagatcg gcgatcttgc
     9541 ccttgcccgg gatcgtggcc tgctcgtcga cgtcctgcca ggggacgagc acgaacgcac
     9601 ggtcgtgggc gcgcgggtgc ggtaggacga gctcctcggt gtcggacaac cggtcgccca
     9661 ccacgatcag gtccacgtcg agggtgcgcg gggcgttgaa ctcggcccgc tcccggccga
     9721 acgcggcttc gatggcctgc gcccggtcga gcagcgtggt gacagacagc gtggtgtcgg
     9781 caagcacgat ggcgttcagg aagtccggag acccgtccgg tgcgtcaacc ggtgcggtct
     9841 cgtacaccgc agacacggtc acgaggtgga cttccggagt gtcagcgagt gcgtccacgg
     9901 caccttgcag gttcgccagc cgatcgccga cattgctgcc caggctgatg acggcggggc
     9961 ggatcggccg catctcgccg gtgagggtgt cggggtcgat gatgtggggg ttcggcgtct
    10021 cagtcatctc ggctccggtg gatggtcagg gtgacgtcgt cgaaggggac ggcgatgggc
    10081 gcatgcggct tgtggaccgt gaccgagacc cacgacaccg gaggttcggc caggcagatc
    10141 tcggccagcc gctgggcgag cgtctcgatg agatcgaccg gatcagaggt gagcgacgag
    10201 tgca
//
LOCUS       DATBFZ010000458        19219 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_1633952, whole genome shotgun sequence.
ACCESSION   DATBFZ010000458 DATBFZ010000000
VERSION     DATBFZ010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 19219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19219
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1633952"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..1214)
                     /locus_tag="VH419_13970"
     CDS             complement(78..1214)
                     /locus_tag="VH419_13970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044772.1"
                     /translation="MAADPSASETVLLLVIVAARLFVPLLIPRLPLMIVAALVLDAVD
                     HTLLETFTDFDTSPTGPYQSVDKALDIYYLSIAYLATMRNWTSDAAFRISQFLFYYRL
                     VGSMLFELTGARAMLLVFPNSFEYFFILYEVIRLRYQPSRFSARFWLWAAAVIWVVVK
                     LPQEYWIHVAKLDFTDTVRDHPVFGIAVGAGLLVAVIVFIVVVLPRLPAPDWSWRLRS
                     DTEPVRVLQSREGVGGRRVLTPATLEQVCLLTLLCIIFANILPGLDATVPQLVLGIGA
                     IVIANAAITAAVHRGRLTPRSAIEHYLARLLTNVVFLYAGNAMLGDRRDFAARYGIFF
                     AFLITTMIWLHDAFYPVHQVRFTDSTLRVRSVADLLRRVREPSP"
     gene            complement(1262..2221)
                     /locus_tag="VH419_13975"
     CDS             complement(1262..2221)
                     /locus_tag="VH419_13975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HEY7044773.1"
                     /translation="MSSAVLLVVGFVALVGGAELLVRAGTGLATWAGIRPMVIGLTVV
                     SIGTSVPELAIGIDAATTGSPGLAVGNIVGTNLVNILLILGLSALILPIAIERRTLRF
                     DLPAMTIASLALFVLALDGNLSKVDGILLCLGGVAYTWGILRTSRREGAAVEAAYSDA
                     IVVPRTQRPVRLALGLLVGIAVIVVGAELLVDGAVATAEGLGISEAVIGLTVVAVGTS
                     APELVTTIVSTVRGDRDIAIGNLVGSTIYNIAIILGLTTLVAPGGIAVPPEIQEADLV
                     LLAVVAVLTVPVFVSGARISRAEGGVFVVVYVAYVIWLLWTRS"
     gene            complement(2237..3487)
                     /locus_tag="VH419_13980"
     CDS             complement(2237..3487)
                     /locus_tag="VH419_13980"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA];
                     GO_process: GO:0019547 - arginine catabolic process to
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEY7044774.1"
                     /translation="MTDTATKLGVHSEVGKLRKVLVCAPGLAHRRLTPTNADDLLFDD
                     VMWVENAQRDHADFVNKMRGRGVEVVELHDLVSETLDIPEAKAWLLDRKIIPNIVGLG
                     LVDDTRAFLESLTSRELAEYLIGGLAVADISDNFQSAYVALAREQIGAREYLMPPLPN
                     TLYTRDTTCWLYGGLTMNPLFWPARHDETLIYKAIYSFHPDFVGSTVWWGDPEQDWGQ
                     ATFEGGDIMPIGNGTVLMGMSERTSRQAITQVAAALFAQQAADRVIVAGMPKLRSAMH
                     LDTVFTFADRDVVTLYPTIMDAVHTFTLLPSDKDPGLEVVDEGGAAFVDVVAKSLGLD
                     GLRVIETGGDVYASERQQWDSGNNAVAVEPGVVFTYDRNTTTNTLLRKAGIEVISIVG
                     AELGRGRGGGHCMTCPLIRDPVDL"
     gene            3765..4826
                     /locus_tag="VH419_13985"
     CDS             3765..4826
                     /locus_tag="VH419_13985"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEY7044775.1"
                     /translation="MLIGALIVCSGLAGALIGALIQGTGSGGASGLAPTPSNATSGSC
                     DTRRIAREVLPAVVTISAKAGAEGGTGSGEIIAADGVVVTNDHVISVAADGGTIEVIL
                     SDGVTKPAELVGRDPRTDLAVLRVSTRSDLPTVEFGDSASLSVGEPVVALGAPLGLSG
                     TVTSGIVSALGRDIPVPTGDGGQTVLAGTIQTDASINPGNSGGALVDCTGGLVGVNTA
                     ILTVPNEAGHGGGGNVGIGFAVPAETVQSVTSQLLDNGRVAHPTFGMATTPIPESTAE
                     EFGTPPGLFVQSVTKGGPADLAGLQRGDIISTIDGQRATQPTVVLHVLATSSVGDQVE
                     IEYYREGQAHSADLTLAAD"
     gene            4858..5181
                     /locus_tag="VH419_13990"
     CDS             4858..5181
                     /locus_tag="VH419_13990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044776.1"
                     /translation="MIMRIWRGVVATDRTKAYVRYIEDTGLAEYLAVEGNRGAQMVTR
                     DLADGRTEVMTVSWWDDLDSVRGFAGDDVEVAKYYPDDDEFLLERDDVVRHYVVAAAP
                     KTSGG"
     gene            complement(5194..6102)
                     /locus_tag="VH419_13995"
     CDS             complement(5194..6102)
                     /locus_tag="VH419_13995"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY7044777.1"
                     /translation="MAKARFKKSTIVRAAERGVPSLLAAAEHVSPSTAARVAERMWFT
                     VPRSAHTVRRTLPAGGRPFAVMVQRHVVRGVRWGDGPVVYLVHGWGGELSQLRAFVAP
                     LVAAGHRVVAFDGPSHGASEAGTSGPGRSDAVALGRALDAVGARFGQPAAIVAHSMGA
                     LSSMLALRDGWLSTPRLALIAPATDLSTYLDRFFETWRIGPRSRRRFEERLEHRVGYA
                     VDDFDVARISTDIARPDLLVVHDAGDRSTTYQGSVDLVQGWPGARLMTAQGLGHRRVL
                     TDQHVVGEVVSFVTVREATVPSDLTA"
     gene            complement(6132..6719)
                     /locus_tag="VH419_14000"
     CDS             complement(6132..6719)
                     /locus_tag="VH419_14000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY7044778.1"
                     /translation="MAKGEETRLAILDEATQVASRIGLSGLTIGRLAQQTQMSKSGLF
                     AHFKSKDVLQVEVLSRARQRFVDTVLAPALAAPRGVPRLRALFEAWLRWDAEILDGGC
                     IFAAASFELDAQPGSARDALVQAERDWLDSLATVAGAAVTEGEFRTDVDCEQFAFELH
                     GLMLAHHHASRLLGDPHATVRTRRAFEFLLERSAA"
     gene            complement(6759..9413)
                     /locus_tag="VH419_14005"
     CDS             complement(6759..9413)
                     /locus_tag="VH419_14005"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEY7044779.1"
                     /translation="MAELAAGQAPYAERARPNLSAYPSPTTARYLIFAVALLSSGLFV
                     GNWIHTEVKGDDWRQTVEACNAAAPADTVGIDQALANQPTFVDCTADVERVRSAFIFG
                     GAAIAAATAFGLLVLTPRIIERRRRLKPLGPGLAGAAERFSLLAAHAGVLRRVRPVIG
                     TAAQRDAFSYGAPGRYRVALPPAVAIRWRNTDTFDPLVAHELSHVARRDVALVWVVRS
                     IWYVLIPILALPLVVGLLVGDPGRVLDYSWRVLVLAAAVLLLSNSLLRSREYDADLGA
                     CRLIGSPDQMSRLLGRTRAAPRTPLWFVRAKHPLPEQRQAAVSAPGVVARMTFLDGLV
                     SGFLTSIAVPLLVSAFTTLLTTFHRTNWTYTVSALIVGPVLGATVGLGLARSALVDRV
                     TGVRSSALPVAAGVGLGFVVGEAVSLAQSSTGRVTGLEHPIWLVVTALVGAGATLLSA
                     SMGQLWADVAPSLRQRRVAWGLGFIVNALIFSSVLWAITQFQFIVDSLGWTIARESLL
                     TALATWPMFVVVVALAVAAAIPLLTTARPVPPPSWLIEAGERRPWIRGGFPQFLATVL
                     VAAITGLVGAACVVAYRAMHGPASELPDQILRFFSYVWVAGLSGAAAAMAISVIANRR
                     GPGAALLATPIAALVTTAGYLVMNETTGGSTTWNFVGLFARPAVVAGFYLTLVVVPAA
                     AIVRLALSRVVGAAGTATTARAVLVASSVVLASVLAVVFGTLALAGREDLVSVDPLAG
                     VDVTPVGPSAHDVVKADFVTYEQTIVPDVMSRYQPIASNLGAIANGGAPGQEVTALDS
                     VVISPLQGLIDDVSAYQPTTREVSDVHAHIIESLNLTMQSAQQWRTAFQTSDPVEAQN
                     ADASAGEAKAAFEAWEQARETLAGELDD"
     gene            complement(9417..9887)
                     /locus_tag="VH419_14010"
     CDS             complement(9417..9887)
                     /locus_tag="VH419_14010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044780.1"
                     /translation="MEVDVDPQLVVELAQISVSKVAPEELAIFDDTVADFFRDPDGTV
                     NPAHRDEAVGFGLDASLATPYVLAVAIPVVQYLVSVVVQTTKEEAGSVVSGWVRRLFR
                     RGDTQPPPPGPALTVEQGKRVRAIAFEKARALGVPDGQAALLADSVSGGLLTAG"
     gene            9998..10294
                     /locus_tag="VH419_14015"
     CDS             9998..10294
                     /locus_tag="VH419_14015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HEY7044781.1"
                     /translation="MIFKGVRDERPYPDHGLRMRDWAELPPHQIRLDQLVTTKDTLDL
                     ATLLDEDSTFFGDLFAHVVEWRGDLYLEDGLHRALRAALQQRHVLHARVHVMRG"
     gene            10335..10868
                     /locus_tag="VH419_14020"
     CDS             10335..10868
                     /locus_tag="VH419_14020"
                     /inference="COORDINATES: protein motif:HMM:NF024791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HEY7044782.1"
                     /translation="MNVRTPVTMVVLLGVLFGAAYYGWQTIVDPDGGNTDTASNGPRG
                     TPCKHKVTIKKGARIQTGDVRVNVYNTGTRSGLAGETLDELVQNGFHRGVADNAPGNL
                     STRNATVLLVHGKAVPQVQLVRKQFIGTVTVHKGPALAPGVDILVGDAFRGVRAAAPK
                     SVRVHKAVTTCAPRRGS"
     gene            complement(10875..12383)
                     /locus_tag="VH419_14025"
     CDS             complement(10875..12383)
                     /locus_tag="VH419_14025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011753794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HEY7044783.1"
                     /translation="MDVDRLDQLLLAGSAVVLLAVLAVRASVRVGLPSLLIYLAMGVL
                     LGESVLGIHFDDASTAQALGFAALVVILTEGGLTTKWSEMRPSLGLGLVLATVGVGVS
                     IVLVAVFAYFVLDFSWQLAFLLGAVTAPTDAAAVFSVLRRVPLKPSILGALEAESGLN
                     DATTVLLVVILSANHDPTGGALGFVGLIAYELVVGIAFGAVIGRLGAAMLRRVALPAS
                     GLYPIAVMAMAALAYSGAAAIHASGFAAVYIAALILGNSDLPHRASTRSFAEGFAWLA
                     QIGLFIMLGLLASPDTFTVQQVVGGVVIGAYLTLIARPISVAVSAAPFRLPWPDQAFI
                     ALAGLRGAVPIVLAAIPLAEHVERAQEEFNVVFVLVVVLTVATAPALPALARRLGVTV
                     DRPRDVEVEAAPLERIAADLLQVHITEHSRLHGVEIGELRLPKGTSVSLIVRGETSFT
                     PDLRTPLRRGDDMLVVTHRKDREATERRLQSVSRGGRLAGWTTGRTARRAGD"
     gene            12518..12934
                     /locus_tag="VH419_14030"
     CDS             12518..12934
                     /locus_tag="VH419_14030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044784.1"
                     /translation="MDKAARVVCLITVAVLAGTPVLAGCGGDDEKPAVCSDVDDIKSS
                     VDDLKNVNLDQSGLSEFQDDLNAVKSDLSKLKSDAEDQYASELDAVDQAFSTLNSSIS
                     AAVSSPSAATITKVQTALQSVGSSLDALDNAVKDTC"
     gene            complement(12938..13837)
                     /locus_tag="VH419_14035"
     CDS             complement(12938..13837)
                     /locus_tag="VH419_14035"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEY7044785.1"
                     /translation="MLAPRLHADQVDIDVPTAGRLLTQQFPEFSDLPLRHVPSGGTDN
                     AVFRIGEGLAMRMPLRPRSVDGLLKEVRWLPTVAPHLTLEVPQIVATGAPTEEYPFAW
                     AVVRWLHGEDALTSRFASMRDAAEAIGRFVAELQRIEPVPAPGSEGFVRGRSLIGRDE
                     AFRAALPKCAGLVDVRRVEQVWDDALSQPDWDGPPVWLHSDLIPGNILLRDGRVVGVL
                     DFGAMCTGDPAYDATAAWSLFDRDHRPLFREIIGVDEPAWCRARGLVVSGGVLALSYY
                     RDTNPSMVAVAHRGISEVLTDAA"
     gene            13969..14163
                     /locus_tag="VH419_14040"
     CDS             13969..14163
                     /locus_tag="VH419_14040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044786.1"
                     /translation="MPEPEGGHVVVLDDTSVLMASSAPQTRAMLEGRGLRCLAVDISE
                     YENLEGCVTCLSVRLRTGPT"
     gene            complement(14188..14277)
                     /locus_tag="VH419_14045"
     tRNA            complement(14188..14277)
                     /locus_tag="VH419_14045"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14241..14243),aa:Ser,seq:cga)
     gene            complement(14338..14802)
                     /gene="tadA"
                     /locus_tag="VH419_14050"
     CDS             complement(14338..14802)
                     /gene="tadA"
                     /locus_tag="VH419_14050"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014847856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HEY7044787.1"
                     /translation="MRDARRRHDEWMRLAIDEARRAETSSDVPIGALVIDPAGEVIGA
                     GHNLREADRDPTAHAEMIAIRQAANSRGEWRLAGCTLVVTLEPCTMCAGAIVLARLDR
                     LVFGALDPKAGAVGSLWDVVRDQRLNHRPQVYGGVLETEAGALIRDFFDVRR"
     gene            complement(14840..15340)
                     /locus_tag="VH419_14055"
     CDS             complement(14840..15340)
                     /locus_tag="VH419_14055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020388612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine deaminase-associated protein"
                     /protein_id="HEY7044788.1"
                     /translation="MCAMDAESTAAIDFAVAAYREDGDWTVSTLPPRAATELDVLVHA
                     LAQLPSDVGTIGMVSVDEDFFVIARVAAGDVRLLLSDVGAATESPLAMAVVDRLDLPM
                     PDDDDDQIQPAGDLGILADLGLAAMELGAMCDDLDLYPDELLGDIASRLGFGRQFDEM
                     LDTATA"
     gene            15433..16074
                     /gene="upp"
                     /locus_tag="VH419_14060"
     CDS             15433..16074
                     /gene="upp"
                     /locus_tag="VH419_14060"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011753771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEY7044789.1"
                     /translation="MQTHVVDHPLVAHKLTALRDVRTESPTFRRLAEELVTLLAYEAT
                     REVRTESVRITTPVADADGVRLAAPKPLVVPILRAGLGMLEGMVRLLPTAEVGFLGMI
                     RDEDTLLASTYAERLPDDLSGRQCFVLDPMLATGGTLSAAIKFLVDRGAHHITAICLL
                     AAPEGCERLEKELAGLDIPVNVVTAAMDEKLNDRGYIVPGLGDAGDRLYGVVG"
     gene            complement(16096..17475)
                     /locus_tag="VH419_14065"
     CDS             complement(16096..17475)
                     /locus_tag="VH419_14065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020389029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HEY7044790.1"
                     /translation="MSGTTAGLCYLVAAVCFILALKGLSSPRTARNGNLVGAAGALLA
                     TIVVLTTDGLQHRVVILVAIAVGTVVGVVAARQVQMTQMPQLVALFNGVGGGAAALVA
                     LLELHESVGASDVPTFNLVATAFTILIGTVSFSGSMVTFAKLQELMTTRPVIFTGLPV
                     LFGAAVVAGVAFSVVLVVDPSMWAGVVIALVGLLLGVLLVLPVGGADVPIVISLLNAF
                     TGLSVAAGGYVLANTLLLIAGTLVGASGTLLTKLMASAMGRSLFNILFGALKGGSTLG
                     GGEVSDRPVRSAGPQDVAILLRYANRVIIVPGYGLAVAQAQHTLRELVDELLERGTVV
                     DYAIHPVAGRMPGHMNVLLAEAQVPYEQLKEMNDINGEFKQADVVLVVGANDVVNPAA
                     RTTPGAPIYGMPILAADEAKQVVFMKRSMRPGFAGIENELLFDPKTTLLFGDAKDTMG
                     KLLSAVKSL"
     gene            complement(17472..17771)
                     /locus_tag="VH419_14070"
     CDS             complement(17472..17771)
                     /locus_tag="VH419_14070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012918298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HEY7044791.1"
                     /translation="MSQADLIWLTIFVLSVFTGIEVISKVSSTLHTPLMSGANAIHGV
                     ILIGAVLVTGASDSTGTTIVGLVAIVLATVNMVGGFVVTDRMLQMFRGRPREPRE"
     gene            complement(17768..18892)
                     /locus_tag="VH419_14075"
     CDS             complement(17768..18892)
                     /locus_tag="VH419_14075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012918297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HEY7044792.1"
                     /translation="MLVAVVKESVEGEARVAMVPELVPTLIGLGYDVGVEPGAGLLAQ
                     YTDDDYAEAGALVDARTLEYADVVVSVQPLDSPRLRQLRSGAATISFFLTLQSLPAVA
                     ERRDLDITSFAMELIPRISRAQSMDALSSQALVAGYRSAIVAAGRLRRFFPLNMTAAG
                     TIPPAEIVVLGAGVAGLQAIATCKRLGAVVKAYDVRAAAAEEIRSMGAIAIELELETL
                     EGAGGYAREMTEDRAERQRQLLAPYIAAADALITTAAVPGRPAPKLVTKAMVEAMGPG
                     SVVVDLAAESGGNVEGSVPGNVVQVGHAQVWGASNVPAQMPGPASKLYAQNLLNLLTL
                     MTGDDGFAPDFDDEVVAGCCVTHAGRVVHEPTREALENAQ"
BASE COUNT         3385 a         6576 c         6463 g         2795 t
ORIGIN      
        1 ttcaggtaac gcgtcagtgc cggtttgtga cgccgcagct ggtgagagcc tcgccacact
       61 ttcaattgcc ggcagggcta cgggcttggt tcgcgaacgc ggcggagcag gtcggccacc
      121 gacctcaccc ggagcgtcga gtccgtgaag cgcacctggt gaaccgggta gaaggcgtcg
      181 tgcagccaga tcatcgtggt gatcaggaag gcgaagaaga tcccatagcg cgcagcgaag
      241 tcgcgacggt ccccgagcat ggcattgccg gcgtacagaa agacgacgtt ggtgaggaga
      301 cgtgccagat agtgctcgat cgccgatcgt ggtgtgagac ggccgcgatg gaccgccgct
      361 gtgatggctg cgttggcgat cacgatggcg ccgatgccca ggacgagctg cggcaccgtc
      421 gcgtcgagcc caggcaggat gttcgcgaag atgatgcaga gcaacgtgag caggcacacc
      481 tgctcgagcg tcgctggggt cagtacgcgt cgtccgccga caccttcgcg ggactgcagg
      541 acccggaccg gctccgtgtc cgacctcagc cgccaggacc agtcaggagc aggaagtcgc
      601 ggcagcacca cgacgatgaa gacgatcacg gcgacgagca accccgcgcc gacagcgatc
      661 ccgaacaccg gatggtcacg caccgtgtcg gtgaagtcga gcttggcgac gtggatccag
      721 tactcctgcg gaagcttgac gaccacccag atgacggccg cggcccacaa ccagaaccgc
      781 gcggagaacc gggacggctg gtagcgcagc cggatgacct cgtacaggat gaagaagtac
      841 tcgaacgagt tcgggaagac cagcagcatc gctcgggcac cagtgagctc gaacagcatc
      901 gacccgacca gccggtagta gaacaggaac tggctgatgc gaaaggccgc atcgctcgtc
      961 cagttgcgca tggtggccag gtaggcgatc gacaggtagt agatgtcgag cgccttgtcg
     1021 acgctctggt acggccctgt cggggacgtg tcgaagtcgg tgaacgtctc gagcagcgtg
     1081 tgatccaccg catcgagcac cagagcggca acgatcatga gcggcagtcg cggtatcagc
     1141 agcggcacga acagtcgcgc ggcaacgatg accagcagca gcacggtttc cgacgccgac
     1201 gggtcggcgg ccaacacagt gatcgagacc agctcctatc cgtccatctc ggcgtgactc
     1261 gtcaactgcg cgtccagagc aaccagatca cgtacgcgac gtagacgacg acgaacactc
     1321 ctccttccgc gcggctgatc cgcgcacccg agacgaagac ggggacggtc agcacggcga
     1381 cgacggccag caggaccagg tcggcctcct ggatctccgg cggcaccgcg atcccgcctg
     1441 gggctacaag agtggtcagc cccaggatga tggcgatgtt gtagatggtc gatcccacca
     1501 gattgccgat cgcgatgtcg cgatcgccac gcacggtgga cacgatggtc gtgaccagct
     1561 cgggtgcgga ggtcccgacg gccacgacgg tgaggccgat caccgcctca ctgatcccga
     1621 gcccctcggc agtcgcgaca gcgccgtcga cgaggagctc ggcgcccacg acgatgacag
     1681 cgatgccgac gagcagtccg agtgcgagcc ggaccggacg ctgcgtacga ggcacgacga
     1741 tggcgtcgga gtaggcagcc tcgactgccg caccttcgcg tcgactcgtc cgcaggattc
     1801 cccaggtgta cgcgacaccg cccaggcaga gcaggatgcc gtcgaccttc gacaggtttc
     1861 cgtcgagcgc gagtacgaac agcgcaagtg acgcgatggt catcgcgggc aggtcgaaac
     1921 gcagcgttct ccgctcgatg gcgatcggca ggatcagcgc gctcaggccg aggatgagca
     1981 ggatgttgac gaggttcgtg ccgacgatgt tgcccaccgc cagaccggga cttccggtcg
     2041 tcgccgcgtc gatgccgatc gccagctcag gcacgctcgt gccgatcgag acgacggtga
     2101 gaccgatcac catcggccgg ataccggccc aggtggccag acctgtgccg gcccgcacca
     2161 acagctccgc tccgccgacc agcgcaacga agccgacgac cagcagcacc gcgctcgaca
     2221 tcgagcaggg tgctggtcag aggtccacag ggtcgcggat gagcggacag gtcatgcagt
     2281 ggccgccgcc gcgcccgcgc ccgagctcgg cgccgacgat ggagatgacc tcgatgccgg
     2341 ccttgcgcag cagggtgttg gtggtggtgt tgcggtcata ggtgaacacg acacccggtt
     2401 cgacagccac ggcgttgttg ccgctgtccc actgttggcg ctcggaggcg tacacatcgc
     2461 ccccggtctc gatcacgcgg aggccgtcga gtccgagcga cttggccacg acgtcgacga
     2521 acgcggcacc tccctcatcg accacctcga ggccaggatc cttgtcgctc gggagcagcg
     2581 tgaacgtgtg gaccgcatcc atgatcgtcg ggtacagcgt gaccacgtcg cggtcagcga
     2641 aggtgaacac ggtgtcgaga tgcatcgccg atcgcagttt cggcatcccg gccacgatga
     2701 cgcgatcggc ggcctgttgc gcgaacaacg ccgccgcgac ctgggtgatg gcctgtcgag
     2761 acgtacgctc actcatcccc atcagcacgg tgccgttgcc gatcggcatg atgtcgccac
     2821 cctcgaacgt ggcctgtccc cagtcctgct ccgggtcacc ccaccagacc gtcgacccga
     2881 cgaagtcggg atggaatgag tagatcgcct tgtagatcag tgtctcgtcg tggcgcgccg
     2941 gccagaagag cgggttcatg gtcagcccgc cgtacagcca acacgtcgtg tcacgggtgt
     3001 agagcgtgtt gggcagcggt ggcatgaggt actcgcgggc gccgatctgc tcacgcgcga
     3061 gggcgacata ggccgactgg aagttgtcgg agatgtcggc gacggcgagg ccaccgatga
     3121 ggtactccgc cagctcacgc gacgtcaacg actccaggaa ggcgcgggtg tcgtcgacga
     3181 ggcccaggcc gacgatgttg gggatgatct tgcgatccag cagccacgcc ttcgcctccg
     3241 ggatgtccag cgtctcgctg accaggtcgt gcagctcgac gacctcgaca ccgcgaccac
     3301 gcatcttgtt cacgaagtcg gcatggtcgc gctgtgcgtt ctccacccac atgacgtcgt
     3361 cgaacagcag gtcgtcggcg ttggtgggcg tgagccgacg gtgcgccagg cccggcgcgc
     3421 agacgagcac cttgcggagc ttgccgacct cggagtggac gccgagcttc gtcgcggtgt
     3481 cggtcatgtg attcctcctg agctcatcga tgcctcgggg tcgacgcgca ggtctcgcgg
     3541 gtggctgaga ccctcagcgc ataccccgcg gcgaacaagg acgactcggc catcatgccc
     3601 cctcttgcgt gcaggttggt gggtccgctg cctcagacca cagggcgcac cgccggcgga
     3661 ctccgcctcc gccagaggct gcccgccgag ccgatccggg cgtaacgtgt cgcgcagccg
     3721 gttccggtcc ggagaagggg tggccagcca cgcagcggcg gaacgtgctc atcggcgcgt
     3781 tgatcgtgtg ttccggtctc gccggagcac tgatcggtgc cttgatccag ggaacaggat
     3841 cgggaggcgc cagcggtctc gcacccaccc cgagcaatgc gacgtcggga tcctgcgaca
     3901 cccgccggat cgcccgcgaa gtcctgccgg cggtcgtgac gatctcggcc aaggcaggcg
     3961 ccgagggcgg gaccggaagt ggcgagatca tcgctgctga cggtgtcgtg gtcacgaacg
     4021 accacgtcat ctcggtcgcc gcggacgggg gcaccatcga agtgatcctc agtgatggcg
     4081 tcacgaagcc cgctgaactc gtgggacgcg atcccaggac cgaccttgcg gtgcttcgcg
     4141 tcagtacacg ctccgaccta cccaccgtgg agttcggcga ttccgcatcg ctcagcgtcg
     4201 gtgaaccggt cgtggccctc ggcgctccac tcgggctgtc gggaaccgtc acatcgggaa
     4261 tcgtcagcgc cctcggccgc gacatccccg tgccgacagg cgacggtggc cagacggtac
     4321 ttgccgggac gatccagacc gacgcgtcga tcaatccagg gaactccggc ggagcgctcg
     4381 tggactgcac aggcggcctc gtcggcgtca acaccgccat cctcaccgtg ccaaacgagg
     4441 ccgggcatgg cggaggcggc aatgtcggca tcgggttcgc cgtaccggcg gagacggtcc
     4501 agtccgtcac gtcgcagctg ctcgacaacg gccgggtcgc acatccgacc ttcggcatgg
     4561 cgacgacccc gatacccgag tcgaccgcgg aggagttcgg aacgccgccc gggctgttcg
     4621 tccagagcgt gacgaagggc ggaccggctg acctggccgg cctccagcgt ggcgacatca
     4681 tcagcaccat cgacggccag cgggccaccc aacccacggt tgtcctgcac gtccttgcca
     4741 cttcgtcggt gggcgaccag gtcgagatcg agtactaccg cgaagggcaa gcacattcag
     4801 ccgacctgac cctggccgca gactgaggtc gtcgtcgggt cgttctaacc ttgaccgatg
     4861 atcatgcgca tatggcgtgg cgtcgtcgcg accgatcgca cgaaggcgta cgtgcgctac
     4921 atcgaggaca cgggtctggc ggaatacctc gcggtggaag gcaaccgcgg cgcccagatg
     4981 gtgacgcgcg acctcgccga cggacgcacc gaggtgatga cggtcagctg gtgggacgac
     5041 ctggactccg tccgcggttt tgccggcgat gacgtcgagg tggcgaagta ctacccggac
     5101 gacgacgagt tcctgctcga gcgggacgac gtggtcaggc actacgtcgt cgctgccgct
     5161 ccgaagacgt caggcggctg agtccccggc acttcaggca gtgaggtcag acggcaccgt
     5221 cgcctcgcgc acggtgacga acgacacgac ctcgccgacg acgtgttggt cggtcagcac
     5281 gcgccggtgc ccgagcccct gcgccgtcat cagccgtgca cctggccacc cctggacgag
     5341 gtcgacactc ccctggtacg tcgtgctgcg gtctcccgcg tcgtgcacga ccagcaggtc
     5401 gggtctggcg atgtccgtcg agatcctggc gacgtcgaag tcgtcgacgg cgtatcccac
     5461 ccgatgctcg agtcgctcct cgaaacgacg acgcgaacgg ggaccgatcc gccaggtctc
     5521 gaagaagcgg tcgaggtacg tcgacaggtc ggtcgccggg gcgatcaacg ccagccgcgg
     5581 cgtcgacagc cagccgtccc gcagcgccag catcgagctc agcgcgccca tcgagtgcgc
     5641 gacgatcgcc gccggctgcc cgaagcgggc tcccaccgca tccagcgccc ggcccagcgc
     5701 gacagcgtcg cttcggccgg ggcctgaggt tccggcctcc gacgcaccgt gactgggccc
     5761 gtcgaacgcg accacgcgat gcccggcggc aaccagcggc gccacgaacg cacgcagctg
     5821 gctcagctcg ccgccccacc cgtgcaccag gtagacgacc ggaccgtctc cccaccgcac
     5881 gccgcgaacc acatgtcgct ggaccatcac agcgaaaggg cggccgcctg ccggcaacgt
     5941 ccgacggacg gtgtgcgccg atctcggcac ggtgaaccac atccgttcgg cgactcgggc
     6001 agccgtcgag ggcgacacgt gctcggctgc ggcgagcaag gacggcaccc ctcgctccgc
     6061 agcacgaacg atcgtgcttt tcttgaatcg tgctttggcc atgaatgtct cccgggactc
     6121 agtgagcgaa ctcaggcggc ggagcgttcg aggaggaact cgaacgcgcg tcgggtgcgg
     6181 acagtggcgt gcggatcgcc aagcaggcgg gacgcgtggt gatgggccag catcaggccg
     6241 tgcagctcga aggcgaactg ctcacagtcg acgtccgtgc ggaactcccc ctcggtgact
     6301 gctgcgcccg cgacggtggc gagcgagtcc agccagtcac gctccgcctg gaccaacgcg
     6361 tcgcgtgcgg accctggttg ggcatcgagc tcgaacgacg ccgcggcgaa gatgcagccg
     6421 ccgtcgagta tctccgcgtc ccaacgcagc caggcctcga acaacgcccg cagccgcgga
     6481 acccctcggg gcgcggccag cgccggcgcg aggacggtgt cgacgaagcg ctggcgggcc
     6541 cggctgagca cctcgacctg caacacgtcc ttggacttga agtgcgcaaa cagaccgctc
     6601 ttcgacatct gcgtctgctg agccaaccgg ccgatcgtca ggcctgacag accgatccgg
     6661 gaggccacct gagtcgcctc gtcgagtatc gcgagtcgcg tctcctcgcc cttcgccata
     6721 cacacataaa agcacgaccg tacttttctg gcaagtcttc agtcgtccag ttcaccagcc
     6781 agcgtctctc tggcctgctc ccacgcctcg aacgccgcct tggcctcacc ggccgacgca
     6841 tcggcgttct gcgcttccac ggggtcgctg gtctggaacg cagtcctcca ttgttgtgcg
     6901 gactgcatcg tcaggttcag cgactcgatg atgtgggcat gcacgtcgga gacctctcgg
     6961 gttgtcggtt gatacgccga gacgtcgtcg atgagcccct gcagcgggga gatcacgacg
     7021 ctgtcgaggg ccgtcacctc ctggccagga gcaccgccgt tggcgatggc ccccaggttc
     7081 gaggcgatgg gttggtaccg actcatgacg tccggaacga tcgtctgctc ataggtgacg
     7141 aagtcggcct tcacgacgtc gtgcgcgctc ggcccgaccg gagtgacgtc cacaccagcg
     7201 agcggatcca cactgacgag atcctctcgg ccggccaggg cgagcgttcc gaagacgacg
     7261 gccaacacgc tcgccagcac gacggacgaa gcgaccagca cggcgcgagc agtggtcgcc
     7321 gtgccggcag ccccgaccac ccgcgacaga gccagccgca cgatcgccgc cgccggcacg
     7381 acaaccaatg tgagatagaa gccggcgacg actgccggcc gggcgaagag cccgacgaag
     7441 ttccacgtcg tgctcccgcc ggtggtctcg ttcatcacca ggtagccggc ggttgtgacg
     7501 agtgccgcga tcggcgtggc cagcaaggcc gcgccaggcc ctcgacggtt cgcgatcacg
     7561 ctgatggcca ttgccgcggc cgcgcccgac agaccggcga cccacacgta ggagaagaac
     7621 cgcaggatct ggtccgggag ttcgcttgcc ggaccatgca tcgcccggta ggcgacgacg
     7681 caggcagcgc ccacgagtcc cgtgatggcc gcgaccagca cggtcgcgag gaactgtgga
     7741 aagccgccgc gaatccacgg tctgcgctcg ccggcctcga tcaaccacga aggcggaggc
     7801 accggccgcg cagtcgtgag cagcgggatg gcagcagcca cggccagtgc gacgacgacc
     7861 acgaacatcg gccaggtggc aagtgcggtc agtaggctct cccgagcgat ggtccagccg
     7921 agcgagtcga cgatgaactg gaactgcgtg atcgcccaca gcacgctgga gaagatcagc
     7981 gcgttgacga tgaagccgag accccaggcc acccgtcgct gccgaagcga cggcgcgacg
     8041 tccgcccaca gctgccccat cgatgccgac aacaatgtcg cgccggcgcc gacgagcgca
     8101 gtgaccacga gccagatcgg gtgctcgagt ccggtgaccc tgccggtgct cgactgagcg
     8161 agcgagaccg cctctccgac aacgaacccg agcccgacgc cggccgcgac cggaagcgcc
     8221 gagctgcgta cgccggtgac gcgatcgacc agcgccgaac gcgccaggcc caggccgacc
     8281 gtggctccga ggaccgggcc gacgatcagt gcgctgacgg tgtacgtcca gttggtgcga
     8341 tggaaggtcg tcagcaacgt ggtgaacgcg gagacgagca acgggacggc gatcgaggtg
     8401 agaaaccccg agacgagacc gtcgaggaag gtcatgcgag ccaccacgcc gggcgctgag
     8461 acggcggcct gccgctgctc gggaagcgga tgcttcgcac gcacgaacca taacggcgta
     8521 cgcggcgctg cccgcgtgcg gccgagcagg cgggacatct ggtccgggct gccgatgagc
     8581 cggcaggcgc ccaggtctgc gtcgtactcc cgcgaacgca gcaggctgtt ggagagcagc
     8641 agcaccgctg cggccagcac caggacccgc cacgagtagt ccaggactct ccccgggtcg
     8701 ccgacgagca gcccgacgac gagtgggaga gccaagatgg ggatcaggac gtaccagatc
     8761 gaccggacga cccagacgag ggccacatcg cggcgggcca catggctgag ctcgtgcgcc
     8821 acgagcgggt cgaacgtgtc cgtgttccgc catcggatgg cgacggccgg tggcagcgcg
     8881 acccggtacc gccccggtgc accgtagctg aaggcatcgc gctgcgcagc cgtcccgatc
     8941 accggccgga cccgacgaag cacccccgcg tgagctgcaa gcaacgagaa ccgctcggcc
     9001 gcgccggcca gcccagggcc gagcggtttc aaccgccggc gccgctcgat gatgcgcggc
     9061 gtcaggacga gcaggccgaa ggcggtggcg gcggcgatcg ccgctccacc gaagatgaac
     9121 gccgaacgga cccgctccac gtctgctgtg cagtcgacga acgtcggttg gttggcgagc
     9181 gcctggtcga tccccaccgt gtcggccggt gcagccgcat tgcatgcctc gaccgtctgc
     9241 cgccagtcgt cacccttgac ctcggtgtgg atccagttgc cgacgaacag tccggaggac
     9301 aacagcgcaa cggcgaagat gaggtaacgg gcggtggtgg gcgatgggta ggcggaaagg
     9361 ttcggccggg ctcgctcggc gtacggggcc tgtcctgccg ccagctcggc catcggtcac
     9421 cccgcggtca gcagaccgcc gctcaccgag tccgcgagca gggcagcctg tccgtcgggg
     9481 actccgagtg ctcgggcctt ctcgaacgcg atcgcgcgca ctcgcttccc ctgctcgaca
     9541 gtcaacgcag gtccaggtgg cggcggttgg gtgtcgccac gtcgaaacag ccgacgtacc
     9601 caccccgaca cgacagatcc cgcctcctcc ttggtggtct ggaccaccac ggagacgagg
     9661 tactggacca cggggatggc gaccgcgagc acgtatggcg tcgccagcga ggcgtccaga
     9721 ccgaacccga ccgcctcgtc gcgatgcgcc gggttaaccg tgccgtcggg atcacggaag
     9781 aagtccgcga ccgtgtcgtc gaagatcgcc agctcctccg gggccacctt ggacaccgag
     9841 atctgtgcca gctcgacgac gagttgcggg tcgacatcga cctccatgcc gtgagactac
     9901 gccgcgcggc aagccggtcc ggttcgtcga ccacgggccg ggactgcgtg tgagggcgag
     9961 tcggaggtat cggggctcgg cccttagact caccgacgtg atcttcaagg gcgttcgcga
    10021 cgagcggccg taccccgacc acgggctacg catgcgcgac tgggccgagc tcccacctca
    10081 ccagatccgc ctcgaccagc tcgtgaccac gaaggacacg ctcgatctgg ccaccctcct
    10141 cgacgaggac tcgacgttct tcggtgacct gttcgcccac gtggtcgagt ggcgtggcga
    10201 cctgtatctc gaggacggcc tgcatcgcgc tcttcgcgcg gccctgcagc agcgtcacgt
    10261 cctgcacgcc cgcgtgcacg tgatgcgcgg ctgacgtcgg gcagacccgc gcagagcgaa
    10321 aggagccgca ggcagtgaac gtgaggacgc cggtcaccat ggtggtgctg ctcggcgtct
    10381 tgttcggtgc ggcgtactac ggctggcaga cgatcgtcga cccggacggt gggaacaccg
    10441 acacggcgtc gaacgggcca cgcggcaccc cgtgcaagca caaggtcacg atcaagaagg
    10501 gggcgcggat ccagaccggc gacgtcaggg tcaacgtcta caacaccggc acgcgcagcg
    10561 gcctggccgg cgagaccctc gatgagctcg tgcagaacgg cttccatcgc ggcgtcgccg
    10621 acaacgcccc cggcaacctc tcgacacgga acgccaccgt gctgcttgtg cacggcaagg
    10681 cggtgccgca ggtgcagctg gttcgcaaac agttcatcgg cacggtgacc gtgcacaagg
    10741 gtccagccct cgcccccggc gtcgacatac tcgtcggcga cgctttccgt ggcgtcaggg
    10801 cggcggcacc caagtccgtc cgtgtgcaca aggccgtgac gacctgcgct ccgcgccgcg
    10861 ggtcctgacc cacgctagtc gcctgctcgc cgggccgttc gtccggtcgt ccatccggcc
    10921 agccgtccgc cgcggctgac ggactgcagc cggcgttcgg tggcctcccg gtccttgcgg
    10981 tgggtcacga cgagcatgtc gtcgccacga cgcagtggcg tccgcagatc gggggtgaaa
    11041 ctggtctcgc ctcgcacgat gagcgagaca ctggtcccct tgggcagccg gagctcaccg
    11101 atctcgacgc cgtgcagcct gctgtgctcg gtgatgtgga cctgcaggag gtccgccgcg
    11161 atccgctcga gcggagccgc ctcgacctcg acgtcacgag ggcggtccac ggtgacaccg
    11221 agccgccgtg ccagagcagg cagcgccggg gcggtcgcca cggtgaggac gacgacgagc
    11281 acgaagacga cgttgaactc ttcttgtgcg cgttcgacat gctcggcgag cgggatggcc
    11341 gcgagcacga tggggacggc accacgcagt ccggcgaggg cgatgaaggc ctggtccggc
    11401 cagggcagtc gaaagggggc agcggagacg gcgacgctga tcggtcgcgc gatgagcgtg
    11461 aggtaagccc cgatcaccac gccgccgacc acctgctgga cggtgaacgt gtccggcgac
    11521 gcgagcagcc cgagcatgat gaacagcccg atctgcgcca gccacgcgaa gccctccgcg
    11581 aacgaccgcg tcgacgcccg gtgaggaagg tcggagttgc cgaggatcag ggcggcgatg
    11641 tacaccgccg cgaaaccgct cgcgtggatg gcggccgcgc cggaatacgc cagggccgcc
    11701 atggccatca cggcgatcgg gtagaggcca gaggccggaa gggcgacacg gcgcagcatg
    11761 gcagcgccga gtcggccgat gaccgcgccg aacgcgatgc cgacgacgag ctcgtacgcg
    11821 atcagaccaa cgaagccgag ggcgcctccg gttgggtcgt ggttggcgct gaggatgacc
    11881 accagcagca cggtcgtggc atcgttgaga cctgactccg cctccaacgc accgaggatt
    11941 gacggtttga gggggacgcg ccggagcacg gagaagaccg cggccgcgtc agtcggggcc
    12001 gtgaccgcgc cgagcaggaa cgcgagttgc cagctgaagt cgagcacgaa gtaggcgaac
    12061 acggcgacca gcacgatgct gacaccgacg ccgacggtgg cgagcaccag accgagcccg
    12121 agggacggac gcatctccga ccacttcgtg gtcagaccac cttcggtgag gatgacgacc
    12181 aacgcggcga agcccagcgc ctgcgccgta ctcgcgtcgt cgaagtggat gccgagcacc
    12241 gactcaccga gcaggacacc catcgcgaga tagatcaaca ggctggggag acccacccgc
    12301 accgatgcac gcaccgccag cacagccagc aggacgacgg cagacccggc cagcagcagc
    12361 tggtccaggc ggtcgacatc cacgcgccca ccctattggg atcccgcccg cccccccgcc
    12421 gccgagagct cccgcgaatt gcgggaaaag tgtccgcgac acgccgatgg agaggtcccc
    12481 tttcccgcaa ttcgggggac gcgagacgat gggcggcatg gacaaagctg ctcgtgtcgt
    12541 gtgtctgatc accgtggcgg tgcttgcggg tacgccagtc ctcgccggct gcggaggaga
    12601 tgacgagaag cccgcggtgt gctccgacgt cgatgacatc aagtcatcgg tcgacgacct
    12661 gaagaacgtc aacctcgacc agagcggcct ctcggagttc caggacgacc tcaacgccgt
    12721 caagtccgac ctgagcaagc tgaagagcga cgccgaagat cagtacgcgt cggagctcga
    12781 cgccgtggac caggcctttt cgacgctcaa ctcgagcatt tcggcggcag tgagctcacc
    12841 gtccgccgcg accatcacca aggtccagac cgccctccag tcggtgggct cgtcactgga
    12901 cgcactcgac aatgcggtca aggacacctg ctgaccgtca ggctgcgtcc gtgagcacct
    12961 cgctgatccc acgatgtgcc actgcgacca tcgaggggtt ggtgtcgcgg tagtacgaca
    13021 gcgcgagcac acctccggag accaccagtc ctcgtgcgcg acaccacgca ggctcgtcga
    13081 ccccgatgat ctcgcggaac agcggcctgt gatcgcggtc gaacagactc caggcagccg
    13141 tcgcgtcgta cgccgggtca ccggtgcaca tggcgccgaa gtcgagcact ccgacgaccc
    13201 gcccgtcgcg gagcaggatg ttgccgggga tgaggtcgga gtgcagccac accggcggac
    13261 cgtcccagtc cggttgtgac agggcgtcgt cccacacctg ctccacgcgg cgtacgtcga
    13321 cgagccccgc acacttcggc aaggccgccc gaaaggcttc gtcgcgaccg atgagcgagc
    13381 ggccgcgcac gaatccctca gatccgggag caggcacggg ctcgatgcgc tggagctccg
    13441 cgacgaaccg gccgatcgcc tcagccgcgt cacgcatgct cgcgaatcgg ctggtcaagg
    13501 cgtcctcgcc gtggagccac cggacgaccg cccaggcaaa cgggtactcc tcggtcggcg
    13561 ctccggtggc gacgatctgg ggtacctcga gcgtcaggtg gggcgcaacg gtcggcagcc
    13621 aacgaacctc cttcagcaag ccgtcgacgg agcggggccg cagcggcatc cgcatcgcta
    13681 agccctcacc gatgcggaac acggcgttgt cggtgccgcc cgacggcacg tgtcgaaggg
    13741 gcaggtcgga gaactcgggg aactgctgcg tcagcagtcg acccgcagtc ggtacatcga
    13801 tgtccacctg gtcagcgtgc agacgaggtg cgagcatgcg gctcatcatc gcggccgagc
    13861 ctcggcgtcg cgaaccgttt tcacccaggg gcttcgaaga tgtcctttct cgcgccatgc
    13921 ttgagccgtg ccgctccgcg ccctggtccg ccgcccgagt tcctggccgt gcccgagccc
    13981 gagggcggac acgtcgtggt gctcgacgac acctcagtgc tgatggcgag cagtgcgccg
    14041 cagacccggg cgatgctcga gggtcgaggc ctgcggtgcc tcgccgtgga catctcggag
    14101 tacgagaacc tcgagggctg cgtgacctgt ctgtcggtcc ggctccggac gggacccacc
    14161 tgagtccggt ggcctcggtg gcttggttgg cggtggcggt gggatttgaa cccacggtgg
    14221 gcttgcaccc acacacgctt tcgaggcgtg ctccttaggc cgctcggaca cgccaccgcg
    14281 cgcaaggcta ccggaggtgc ccgagcgtgc agaaatcggc ggacccgccg acggcgctca
    14341 ccggcgaacg tcgaagaaat cgcgtatcaa cgctcccgcc tcggtttcga gcacgccgcc
    14401 gtacacctgt ggacggtgat tgagacgttg gtcacgcacg acgtcccaca aggatccgac
    14461 tgccccggcc ttcgggtcca acgcgccgaa caccaggcgg tcgagcctgg ccagcacgat
    14521 ggctccggcg cacatcgtgc agggctcgag cgtgaccacg agcgtgcatc cggccagccg
    14581 ccattcgccc ctgctgttcg ccgcctgccg gatcgcgatc atctccgcgt gagcggtcgg
    14641 atcccggtcg gcctcgcgga ggttgtggcc ggctccgatg acctcgccgg cagggtcgat
    14701 gacgagcgcg ccgatcggta cgtcgcttga cgtctcggcc cggcgagcct catcgatggc
    14761 cagccgcatc cactcgtcgt gtcgacgacg tgcatcgcgc atcggcgtac tcacgaaatc
    14821 gcgagatccg gtaacccggc tacgccgtcg cggtgtcgag catctcgtcg aactgccgtc
    14881 cgaacccgag tcgcgatgcg atgtcgccga ggagctcgtc gggatacaga tcgaggtcgt
    14941 cgcacatcgc gcccagctcc atcgccgcca agccgagatc ggcgaggatg ccgaggtctc
    15001 cggccggttg gatctggtcg tcgtcatcgt cgggcatcgg gaggtcgagc cggtcgacga
    15061 cggccattgc gagtggtgac tcggtggcgg ccccgacgtc ggagagcagc aaccgcacgt
    15121 cgccggcggc gacacgcgcg atgacgaaga agtcctcgtc cacgctcacc atcccgatcg
    15181 ttcccacgtc ggaagggagc tgcgcgagcg cgtgcacgag gacgtcgagc tccgttgctg
    15241 cgcggggtgg aagcgtggac acggtccagt caccgtcctc gcggtaggcg gctacggcga
    15301 agtcgatggc ggctgtggac tcggcatcca tcgcgcacat acatcgatgg tgacagacga
    15361 ggtgagcctc tggcgagccc cggcagtgcc ctctcgaggg gacggttgcg gtgatcgcct
    15421 agggtcgccc tcatgcagac tcacgtcgtc gaccacccac tcgtcgccca caagctcacc
    15481 gccctccgag acgtccggac cgagtcgccg acgttccgcc ggctggccga ggagctggtg
    15541 accttgctcg cgtacgaggc cacgcgggag gtcaggacgg agtcggtccg catcacgacg
    15601 ccggtcgcgg acgccgacgg tgtccgtctt gctgcgccga agccgctcgt cgtaccgatc
    15661 ctgagggcag gactcggcat gctcgagggg atggtccggt tgctgccgac ggccgaggtc
    15721 gggttcctcg gcatgattcg cgacgaggac acgctgctgg cttcgacgta cgccgagcgg
    15781 cttccggacg acctctccgg ccggcagtgc ttcgtgctgg atccgatgct ggcgacgggt
    15841 ggcacgctgt cggcggcgat caagttcctg gtcgaccggg gggcgcacca catcacagcg
    15901 atctgcctgc tggcggcccc tgagggttgc gagcggctgg agaaggagct ggccgggctg
    15961 gacatcccgg tgaacgtggt gaccgcggcg atggacgaga agctcaacga ccggggctac
    16021 atcgtgcctg gactcggcga cgccggtgac cgcctctacg gcgtcgtcgg ctgaccggcc
    16081 acttgctggt cgtcgtcaca gcgacttgac ggcggagagc agcttgccca tggtgtcctt
    16141 cgcgtcgccg aagagcagcg tcgtcttcgg gtcgaacaac agctcgttct cgatgccggc
    16201 gaacccgggc cgcatcgaac gcttcatgaa cacgacctgc ttcgcctcgt ccgccgcgag
    16261 gatcggcatc ccgtagatcg gcgcgccagg agtcgtcctc gccgccggat tcacgacgtc
    16321 gttcgcaccc accaccaaga cgacgtcggc ctgcttgaac tcgccgttga tgtcgttcat
    16381 ctctttcagc tgttcgtacg gcacctgcgc ctccgcgagc agcacgttca tgtgcccggg
    16441 catgcggccg gccaccggat ggatggcgta gtcgaccacg gtccctcgct cgaggagctc
    16501 gtcgacgagc tcgcgcaacg tgtgctgcgc ctgcgccacc gcgaggccgt agcccggcac
    16561 gatgatgacc cggttcgcat atctcagcag gatcgccaca tcctgcgggc ctgcggaacg
    16621 caccggacgg tccgacacct cgccgccacc gagcgtcgag ccacctttga gcgcgccgaa
    16681 caagatgttg aacagcgacc gtcccatcgc cgacgccatc agcttcgtca gcagcgttcc
    16741 ggacgcgccg accagggtgc ccgcaatcag cagcagcgtg ttggcgagca cgtagccgcc
    16801 ggcagcgacc gagagtccgg tgaaggcgtt cagcagcgag atgacgatgg ggacgtcggc
    16861 tccgccgact gggagcacga gcagcacgcc cagcagcagc ccgaccagcg cgatcacgac
    16921 gcccgcccac atcgacgggt cgacgacgag cacgactgag aacgcgaccc cggcgacgac
    16981 cgccgcgccg aacagcacgg gcagtccggt gaagatgacg ggcctggtcg tcatcagctc
    17041 ctggagcttc gcgaacgtga ccatggagcc ggagaagctg acggtcccga tcaggatcgt
    17101 gaacgcggtg gcgaccaggt tgaacgtcgg tacgtcggag gcacccacgc tctcgtgcag
    17161 ttcgagcagc gccaccagag cggccgcgcc accgccgact ccgttgaaca acgcgaccag
    17221 ctgcggcatc tgcgtcatct gcacctgccg ggcagcgacg acgcccacca ccgtccccac
    17281 ggcgatcgcc accaggatca cgacccgatg ctgcagcccg tcggtcgtca gcaccacgat
    17341 cgttgcgagc aaggcaccgg ctgcaccgac caggttgccg ttcctggcag tacgcgggga
    17401 cgagagcccc ttgagcgcca ggatgaagca gacggccgcg accagatagc acaggccggc
    17461 cgtcgtaccg ctcattcgcg cggctccctc ggccgtccgc ggaacatctg cagcatccgg
    17521 tcggtgacca cgaaaccacc gaccatgttc acggtggcga gcacgatcgc gaccaggccc
    17581 acgatcgtcg tcccggtcga atcactcgcc cccgtcacca gcaccgcgcc gatcaggatg
    17641 acgccgtgga tggcgttcgc acccgacatc agcggcgtgt gcagcgtcga cgagaccttc
    17701 gagatcacct cgatgccggt gaacacgctc agcacgaaga tcgtcaacca gatcaggtcg
    17761 gcttggctca ttgggcgttc tccaatgctt cccgggtggg ctcgtgtacg acgcggccgg
    17821 catgcgtgac gcagcagccg gcgaccacct cgtcgtcgaa gtccggggcg aaaccgtcgt
    17881 cgcctgtcat cagcgtcagc aggttgagga ggttctgcgc gtacagcttg ctcgccggtc
    17941 cgggcatctg ggccggtacg ttcgacgccc cccagacctg cgcgtgaccg acctggacga
    18001 cattcccggg tacggatcct tcgacgttgc cgcccgactc cgccgccagg tcgaccacga
    18061 cggaccccgg acccatcgcc tccaccatcg ccttggtcac gagcttcggg gccgggcgac
    18121 ccggcacggc cgccgtcgtg atcagggcgt cggccgcggc gatgtacggc gccaacagct
    18181 gccgctgccg ctcggcacgg tcctccgtca tctcacgggc gtagccgccc gcgccctcca
    18241 gcgtctcgag ctcgagctcg atcgcgatcg cgcccatcga cctgatctct tcggcggctg
    18301 ccgcccggac gtcgtacgcc ttgaccaccg cgccgagccg tttgcaggtg gcgatcgctt
    18361 gcagaccggc cacaccggcg cccagcacga cgatctcggc gggggggatt gtgcccgctg
    18421 ccgtcatgtt cagcgggaag aaccggcgga gccgcccggc cgcgacgatg gcggatcggt
    18481 aaccggccac gagcgcctgc gagctcagcg catccatgga ctgcgcgcgg gagatccgcg
    18541 ggatcagctc catcgcgaac gacgtgatgt cgaggtcgcg gcgctccgcg acggccggca
    18601 gtgattgcag ggtcaggaag aaggagatgg tcgcggcgcc ggaccgcagc tgacgcaggc
    18661 gcgggctgtc gagcggctgc acggacacga ccacgtcggc gtactccagc gtccgcgcat
    18721 cgaccagcgc gcctgcctcg gcgtagtcgt cgtcggtgta ctgcgccaac agccccgcgc
    18781 ccggctcgac gcccacgtcg taccccaacc cgatcaacgt gggcaccagc tccgggacca
    18841 tggcgacacg tgcctcgccc tccacggact ccttcacgac ggccacaagc acgtgccgaa
    18901 cctagagcac agccgacgcg cacgacacca ccgggtcgag tgtcgtcagt agtgatccgg
    18961 gaaccccggc aggctcgagg ctgttgacgg tcgtcggctt tcgcgcgacc aagcgctccg
    19021 acggaggtgg cacgagcccc gctccactgc ctagccaact ccaaactgac cgtcaccgcg
    19081 ttgtgatcga atcttgtgga tcgatgttcg acacggccct cggtcgtgta atacttgagt
    19141 cctagctccc caccgtcctc cgggactggt ggggtttttt ttctgcccgc aggagggtct
    19201 cgtggctgac ctccggact
//
LOCUS       DATBFZ010000459         1555 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_1964915, whole genome shotgun sequence.
ACCESSION   DATBFZ010000459 DATBFZ010000000
VERSION     DATBFZ010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1964915"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..649
                     /locus_tag="VH419_14080"
     CDS             <1..649
                     /locus_tag="VH419_14080"
                     /inference="COORDINATES: protein motif:HMM:NF012613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HEY7044793.1"
                     /translation="APPLPPGSLNDVVVMNPDTTTGTLLKGWVADFLDVPDPHQFHDF
                     VTTLVSNGVTVGCGFGNYCVDNPTTRAHMAVFLLKALLGFCFTPPPATGTVFADVPAT
                     SIYAPWIEELARRGLTAGCGGGNYCPDASVTRAQMAVFLLNTAYGPGYAPPPATGTAF
                     DDVPAGSFAADWIEDLVARGITAGCSASPPLYCPANPITRGQMATFIVITFALVP"
     gene            687..>1555
                     /locus_tag="VH419_14085"
     CDS             687..>1555
                     /locus_tag="VH419_14085"
                     /inference="COORDINATES: protein motif:HMM:TIGR03803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor tandem repeat GloVer-containing
                     protein"
                     /protein_id="HEY7044794.1"
                     /translation="MRFRVAAAWLVRCATGFAVFVASSRGARAQTYEVLHDFTGTNGD
                     GAIPYGTLVQVGSDFYGTTRSGGTTALCTAGCGTIFKLDSGGHVTTVYSFDADTDDGL
                     TPIAGLTLATDGNLYGTTPVGGATANAAVYRFEPGTGLVTTIDSFDRDTGGWFGSTNA
                     MEGTDGQLYVATTYGHCGDDTDNCPQVLRMTLSGSATPFHTFDPEAEGSMPLSPLLQT
                     SPGDFYGTTTGGNGAVFHLNAAGEWTLVHGFNDTDGRVPMGNLVRDGAGNIYGVTAFG
                     TESNLGTIYRIAA"
BASE COUNT          249 a          573 c          499 g          234 t
ORIGIN      
        1 ggctccgccc cttccgcccg gctccctgaa cgacgtcgtg gtgatgaacc cggacacgac
       61 cacgggcacg cttctcaagg gctgggtcgc cgacttcctc gacgttccgg acccgcacca
      121 gttccacgac ttcgtcacga cgctcgtctc gaacggggtg acggtcggct gcggcttcgg
      181 aaactactgc gtcgacaacc cgacgacgcg cgcgcacatg gcggtcttcc tcctgaaggc
      241 gctcctcggc ttctgcttca cgccgccgcc cgcgaccggg accgtgttcg cggacgtccc
      301 ggccacgagc atctacgcgc cgtggatcga ggagctggcg cgccgcgggc tcaccgcggg
      361 ctgcggcggc ggcaactact gcccggacgc ctccgtgacg cgcgcgcaga tggcggtctt
      421 cctcctgaac accgcctacg ggccgggcta cgcgccgcca cccgcgacgg ggaccgcctt
      481 cgacgacgtg ccggccggct ctttcgcggc cgactggatc gaggacctcg tcgcgcgggg
      541 catcaccgcg ggctgcagcg cctcgccgcc cctctactgc cccgccaacc cgatcacgcg
      601 cggccagatg gccacgttca tcgtgatcac gttcgcgctc gtgccatgag gcccggaggc
      661 cgagggagga cgagatgaga aaggcgatgc gtttccgagt cgccgccgcg tggctggttc
      721 ggtgcgcgac gggattcgcc gtcttcgtcg catcgtctcg gggcgcccgg gctcagacgt
      781 acgaagtcct ccacgacttc accgggacga acggcgacgg cgccatcccg tacggcacgc
      841 tggtccaggt cggaagcgac ttctacggca cgacgcgctc cggcggtacg accgctctct
      901 gcaccgccgg ctgcggcacg atcttcaagc tggactcggg cggccacgtc accacggtgt
      961 attcgttcga cgcggacacc gacgacgggc tcacccccat cgccgggctc accctcgcga
     1021 ctgacgggaa tctctatggg acgacgcccg tcggaggcgc gaccgcgaac gcagccgttt
     1081 accgcttcga gccgggaacc gggctggtca ccacgatcga cagcttcgat cgagacaccg
     1141 gaggatggtt cggatccacc aatgccatgg aggggacgga cggccagctc tacgtcgcta
     1201 cgacctacgg ccactgcggg gacgacaccg acaattgtcc ccaggtcctt cgaatgacgc
     1261 tatccggaag cgccaccccc ttccacacgt tcgatcccga agccgaaggc tccatgccgc
     1321 tctcgccgct cctgcagacg agccccggcg acttctacgg cacgacgacc ggcgggaacg
     1381 gagccgtgtt tcacctcaac gccgcgggcg agtggaccct ggttcacggc ttcaacgaca
     1441 cggacggccg ggtgccgatg gggaacctgg tgcgcgacgg cgccgggaac atctacggcg
     1501 tcacggcctt cgggaccgag tccaacctcg gaacgatcta tcgcatcgcc gcgaa
//
LOCUS       DATBFZ010000460         4068 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_3603310, whole genome shotgun sequence.
ACCESSION   DATBFZ010000460 DATBFZ010000000
VERSION     DATBFZ010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 4068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4068
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3603310"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1283)
                     /locus_tag="VH419_14090"
     CDS             complement(<1..1283)
                     /locus_tag="VH419_14090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044795.1"
                     /translation="MRVRLSNALLIAVLLAASQTAFAAERAQQNDSRYFAETGFRIAN
                     DKFWEFFQGRGGTKTFGYPTSREMTLKGFTVQFFQRGVMQLGADGVETLNLLEDGLLP
                     YTTINGSTFPAPDPAMTKAAPVPSDPDYASKIIDFVKENAPDTWEGMPVNFNKTFTST
                     VSYDAAFPRGDGPASLLPLFNLQIWGAPTSKPLRDPKNNNFVYLRFQRGIMHYDDGCK
                     CTQGLLLGDYLKALITGENLPADLEAQAADSPLLRQYSKSDSAGLARAGDLANSNFKD
                     AFEKVSPGQATAAAPGAPASSGAANSEAASKAAAQAAAKPSTSAGTSSSTASKPSTAA
                     SPPPAAGLPERAKSPEYGMNVFIWGNKATTDRDLKKLSDAGFGWQKTLFQWRFIEPQK
                     KGQFDWDEADRIVKASKANGIKILARLDQQPAWARA"
     gene            1634..2101
                     /gene="greA"
                     /locus_tag="VH419_14095"
     CDS             1634..2101
                     /gene="greA"
                     /locus_tag="VH419_14095"
                     /inference="COORDINATES: protein motif:HMM:NF001263.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEY7044796.1"
                     /translation="MPDRAVYLTREGFKKLDGELEHLRTVKRPEVSERIRRSKDMTDV
                     VDNAEYDEAKSEQGFIEGRIAELELTLASAQIIGDEPSGDFVGLGSRVTVRDDEGDTE
                     QYRIVGSMEADPKRGLISNESPVGRALLGKKPGESATVVAPGGSFDLVVVEVG"
     gene            2413..3921
                     /gene="lysS"
                     /locus_tag="VH419_14100"
     CDS             2413..3921
                     /gene="lysS"
                     /locus_tag="VH419_14100"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEY7044797.1"
                     /translation="MTHDDAGSSNGHDADRPDDRLFEQRLDKLNRLRAAGIDPYPARF
                     ERTDLAADVVAGFEATPGRQVRVAGRVVGGIRRMGRSTFLHLQDGSGRIQAYFKQDVV
                     GPERYALLELVDAGDFLGVVGETMRTRSGEVTVQASAFEVLSKALRPLPEKWHGLQDV
                     ETRYRKRYLDLIANPEVMEVFRARGRIVQEVRNFLVERGFLEVETPVLQTVPGGGSAR
                     PFETFYNALDRTLSLRIALELYLKRCVVGGIEKVFEIGRNFRNEGISLKHNPEFTMLE
                     VYQAYADYEEIMRLTEDLVSTVAQRALGTTKVVVKGDVEVDLSPPWERKPLHDAIYEA
                     SGVDYDEHLTAESLAKAAAEAGLRVQPDWTRGKIVDELLSVFVEPKITSPVFLVDYPL
                     ELSPLAKQRPDRPDIVERFEAFCGGMEIANAFSELNDPLDQRERFMQQARQKGAGDDE
                     AMPFDADFLEALEHGMPPTGGMGLGIDRLTMVLTGQDKIRDVVLFPQMRTVE"
BASE COUNT          641 a         1290 c         1481 g          656 t
ORIGIN      
        1 gcgcgggccc aggccggctg ctggtcgagc cgggccagga tcttgatgcc gttcgccttg
       61 gacgccttga cgattcggtc ggcctcgtcc cagtcgaact gccccttctt ctggggctcg
      121 atgaagcgcc actggaacag cgtcttctgc cagccgaagc cggcgtcgga cagcttcttc
      181 agatcacggt cggtcgtcgc cttgttgccc cagatgaaca cgttcatgcc gtattcgggg
      241 ctcttggccc gctccgggag gccggcggcc ggtggagggc tggctgccgt gctcggtttg
      301 ctggcggtgc tgctggacgt cccggccgac gtggacggct tcgccgctgc ctgggccgcc
      361 gccttcgagg ccgcttcgga gttggcggcg cccgacgagg ccggcgcgcc cggcgcggcg
      421 gccgtcgcct ggcccggcga gaccttctcg aaggcgtcct tgaagttgga gtttgccagg
      481 tcgccggccc gggccagccc ggccgagtcg ctcttcgagt actggcgcag cagcggactg
      541 tcggccgcct gcgcttccag gtcagccggc aggttctcgc cggtgatcag cgccttcagg
      601 tagtcgccga gcagcagccc ctgcgtgcac ttacagccgt cgtcgtagtg catgatgccg
      661 cgctggaacc ggagatacac gaagttgttg ttcttcgggt cgcggagcgg cttcgaagtc
      721 ggcgcgcccc agatctggag gttgaagagg ggcagcaggc tggccggacc gtcgccgcgt
      781 ggaaaggcgg cgtcgtagct gacggtgctg gtgaacgtct tattgaagtt gaccggcatg
      841 ccttcccagg tgtccggggc gttctccttg acgaagtcga tgatcttcga ggcgtagtcc
      901 gggtcgctcg ggaccggcgc ggccttggtc atggccgggt cgggagccgg gaacgtcgag
      961 ccgttgatcg tggtgtacgg cagcaggccg tcttccagca ggttgagcgt ctcgacgccg
     1021 tcggccccga gctgcatcac accgcgctgg aagaactgga cggtgaagcc tttgagggtc
     1081 atctcacgag aggtcggata gccgaacgtc ttggtgccgc cacggccctg gaagaactcc
     1141 cagaacttgt cgttggcgat tcggaatcct gtttcggcga agtagcggga gtcgttctgc
     1201 tgggctcgtt cggcggcgaa ggcagtctgt gatgccgcga gaagaacggc gatcagcagc
     1261 gcgttggata gacggacgcg catccggggc tcctccgtgc ggtgcggcgc cgccctgcac
     1321 ggggagggcc cgtggcgccg cgggattata cggaagccca tttcgggcca caagactttc
     1381 cgtcccgctc gtggcgagga acgtctgacg ggccccatcc gtcgcgattc gacgctggaa
     1441 ggtgaacgtc ggggtctgac cggacgggac gagcgtcgta tatcttgaca agaagcgaca
     1501 cggagctata atccgtttcc caaacacggc gcgggctggt cccgcgccgc gctcgtcgtc
     1561 gcggcgacga cgagtggcac ggtgacgacg cgcgagcacc gactgatcgg atgtgggaag
     1621 gacggacctg gcaatgcctg accgtgcggt gtacctgacc cgcgaggggt tcaagaagct
     1681 cgacggcgag ctggagcacc ttcgcacggt caagcgcccg gaggtctcgg agcgaatccg
     1741 tcggtccaaa gacatgaccg atgtggtcga caacgccgag tacgacgagg cgaagagcga
     1801 gcagggcttt atcgagggcc ggatcgccga gctggagctg acgctggcga gcgcccagat
     1861 catcggcgac gagccgagcg gcgactttgt cgggctgggg tcccgcgtga ccgtgcggga
     1921 cgacgagggg gacaccgagc agtaccggat cgttggctcg atggaggcgg acccgaagcg
     1981 gggcctgatc tcgaacgagt caccagttgg ccgggcgctg ctcggcaaga agcccggcga
     2041 gtcggccacg gtggtggcgc cgggcggcag cttcgacctg gtggtggtcg aggtcgggta
     2101 agcccggggc cgtgggttga cgtcggcggc gacccggggc cgtggactga agtccacgtc
     2161 taccatcgtg tgtggctccg ccacaccggg gcggggtggc ccgtccctcg cctggtcgcc
     2221 gagcgaccac cgaccgtagc catgggttac aacccacgaa cctggcttgg cgcggcgtcg
     2281 agtcctcggc ccgagcgtgc ggccactccg gtacacttca agaagacttc ccccagagcg
     2341 gacgttcgcg tccgctcccc actacccggc gcggggccca ccctcccgcc ctggcgccga
     2401 cggagctacg ccgtgacaca tgacgacgcc gggtcgtcga acggccacga cgccgaccga
     2461 cccgatgacc gactgttcga gcagcgcctc gacaagctga accgactccg cgcggccggg
     2521 atcgatccgt atccggcccg cttcgagcgg accgacctgg ccgccgacgt cgtcgccggc
     2581 ttcgaggcca cgcccggccg gcaggtccgg gtggccgggc gcgtcgtcgg cgggatccgg
     2641 cggatgggcc gctcgacctt tctccatctc caggacggca gcggcaggat tcaggcgtac
     2701 ttcaagcagg acgtggtcgg ccccgagcga tatgcgctgc tggagctggt tgacgcgggt
     2761 gacttcctgg gcgtggtcgg cgagacgatg cggacccgca gcggcgaggt gaccgtccag
     2821 gccagcgcat tcgaggtgct ctcgaaggcg ctccggccgc tgccggagaa gtggcacggc
     2881 ctccaggacg tcgagactcg ctaccgcaag cggtaccttg acctgatcgc caatcccgag
     2941 gtgatggagg tgttcagggc gcgcggccgg atcgtccagg aggtccggaa cttcctggtc
     3001 gagcgcggct tcctggaggt cgagacgcca gtcctccaga ccgtccctgg gggaggatcg
     3061 gcccgcccgt tcgagacgtt ctacaacgcg ctcgacagaa cgctgtcgtt gcggatcgcc
     3121 ctggagctgt acctgaagcg ctgcgtcgtc ggcggcatcg agaaggtctt cgagatcggg
     3181 cgcaacttcc gcaacgaggg catctcgctc aagcacaacc ccgagttcac gatgctggag
     3241 gtgtaccagg cgtacgccga ctacgaggaa atcatgcggc tgaccgaaga cctcgtctcg
     3301 acggtggcgc agcgggcgct cgggacgacg aaggtcgtcg tcaagggcga cgtcgaggtt
     3361 gacctctcac cgccgtggga gcgcaagccg ctccacgacg ccatctacga ggcgtccggc
     3421 gtggactatg acgagcacct gacggcggaa tcgctggcga aggcggcggc cgaggccggc
     3481 ctgcgggtcc agccggactg gacgcgcggc aagatcgtcg acgagctgct gagcgtcttc
     3541 gtggagccga agatcaccag cccggtcttc ctggtggact atccgttgga gctgtcgccg
     3601 ctggcgaagc agcgccccga ccggcccgac atcgtcgagc gcttcgaggc gttctgcggc
     3661 gggatggaga tcgccaacgc cttcagcgag ttgaacgacc cgctggacca gcgcgagcgg
     3721 ttcatgcagc aggctcgaca gaagggggcc ggcgacgacg aggcgatgcc gttcgacgcc
     3781 gacttcctgg aggcgctgga gcacgggatg ccgccgaccg gtggcatggg cctcgggatc
     3841 gaccgcctga cgatggtcct gacgggccag gacaagatcc gcgacgttgt cctgttccca
     3901 cagatgcgga ccgtggagta gggccgtgct accgatgacg ctgatccgat cccagccgga
     3961 ggtcgtgcgt gaggcgctgc ggcgccggcg cgaggacgaa tggctggtgg acgccgtgct
     4021 ggaggaggac gcgcgccggc gggcgctggt cgacgagcgc gacaacct
//
LOCUS       DATBFZ010000461         4786 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_322657, whole genome shotgun sequence.
ACCESSION   DATBFZ010000461 DATBFZ010000000
VERSION     DATBFZ010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 4786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4786
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322657"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="VH419_14105"
     CDS             complement(<1..485)
                     /locus_tag="VH419_14105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020384823.1"
                     /note="catalyzes the formation of D-glucono-1,5-lactone
                     6-phosphate from D-glucose 6-phosphate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEY7044798.1"
                     /translation="MTTLETSGSVNPLRDPEDRRLPRIAGPCGLVIFGVTGDLSRKKL
                     MPAVYDLANRGLLPPGFSLVGFARRDWADQDFAQIVHDAVSDYSRTPFREEVWRQLAE
                     GFRFVPGDFADDVAFDKLRETIHELDVARGTGGNHAFYLSVPPKFFPDVLGQLKEHGL
                     A"
     gene            complement(482..2086)
                     /locus_tag="VH419_14110"
     CDS             complement(482..2086)
                     /locus_tag="VH419_14110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020384822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEY7044799.1"
                     /translation="MKIPLTVTTSTAYDDQVAALVERQLASKIAAQDPTLWGPDAESE
                     AAIRLSWVDLPTSSRPLLAEIDALRAQLWSEGLDQVVLCGMGGSSLAPEVISRTYDVP
                     LTILDSTNPSVIQRAVSADLTRTVVVVSSKSGGTLETDSQRRIFAAAFEKAGIDAASR
                     FVVVTDPGSDLEKLATEQGYRKVFLADPHVGGRYSAMTAFGLVPTGLAGIDVAEVLDE
                     AEAVTENVRFDSPDNPALLLAVALAAAGRRDKAAIADLGSGIVGFADWAEQLIAESTG
                     KLGKGVLPIAVGDASAAEVRQPPADVVAVALVSPDAATSTPDGVGEPWVTVSGTLGAQ
                     FLAWETATAVLGHLLGIDPFDQPDVESAKKAARGLLDSRPEESPAQITEGEVEARGSD
                     GLLDGVDTISGAVESLLSRLGPDGYLAVMAYLDSDRDAALHGVRDTLSRRTGRPTTFG
                     WGPRFLHSTGQYHKGGPGQGVFLQITSTEPTDIDIPDRPFSFGTLIAAQAAGDASVLA
                     DHGRPVLRLHLNDPATGVTALQELLR"
     gene            complement(2083..3207)
                     /gene="tal"
                     /locus_tag="VH419_14115"
     CDS             complement(2083..3207)
                     /gene="tal"
                     /locus_tag="VH419_14115"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012920888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEY7044800.1"
                     /translation="MNDRLQALSDQGVSVWLDDLSRERLHTGNLERLVAERHVVGVTS
                     NPTIFAKALSDADDYAEQVKDLAVRGVELEEAVRAITTYDVRWACDVLRDAYDRTDGQ
                     DGRASIEVDPRLAHKTDATIAEARALWWLVDRSNVMIKIPATEEGLAAITTATSEGIC
                     VNVTLIFSLARYRAVMDAYLSGLEQAHSKGVDLSTIRSVASFFVSRVDTEVDKRLDAI
                     IESGQRVEQAKALRSKAAVANARLAYREYDEVFTSDRWSALEAAGANKQRPLWASTSV
                     KDKSLPDTMYVDELIAPDTVNTMPEGTIEAALDHATVDGDTVTTNYDDAEAVLDGLER
                     LGISYEDVVKVIEDEGVDKFEKSWQELLDSVREALGGAKR"
     gene            complement(3276..>4786)
                     /locus_tag="VH419_14120"
     CDS             complement(3276..>4786)
                     /locus_tag="VH419_14120"
                     /EC_number="2.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016744 - transketolase or transaldolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEY7044801.1"
                     /translation="SIEDDTAISFSEDVAKRYAAYGWHVQSIDWTNDATAYQENVRGL
                     FQAFKRAKAETRRPSFIRLRTIIAWPAPNAQNTGEAHGSALGDEEVAATKKVLGFDPD
                     KTFEVADDVISHTRQAIERGKAEQAEWQERFDAWAAKAGDRKELFERMQTRTLPTGWT
                     DALPTFDADAKGVATRKASGEILSAIAPRLPELWGGSADLAGSNNTTPKGEPSFIPSE
                     HATKMFSGDPYGRVLHFGIREHAMGSLLNGIALHGGTRPYGGTFLIFSDYMRPAVRLA
                     ALMKLPVIYVWTHDSIGLGEDGPTHQPIEHLAALRAIPGLDVVRPADANETVAAWRTV
                     LEHTDRPAGLALTRQNVPTFPRGTDGYADTSNVSRGAYVLLDVDGGAPDVVLIGTGSE
                     VQIAVAAREQLAADGIRARVVSMPCREWFEEQEKAYRDSVIPPTVKARVSVEAAVPLG
                     WRDIVGDAGRMVGIDHFGASADYQRLFMEFGLTSEVVTQAAKDSIAAADAKG"
BASE COUNT          676 a         1666 c         1653 g          791 t
ORIGIN      
        1 tcggccaggc cgtgctcctt gagctggccc agcacgtcgg ggaagaactt gggcggcacg
       61 gacaggtaga acgcgtggtt cccgccggtg ccgcgggcca cgtcgagctc gtggatggtc
      121 tcccggagct tgtcgaaggc cacgtcgtcg gcgaagtcgc ccggcacgaa ccggaagccc
      181 tcggccagct gccgccacac ctcttcgcgg aacggcgtgc gggagtagtc gctcaccgcg
      241 tcgtggacga tctgggcgaa gtcctggtcg gcccagtcac gccgtgcgaa cccgaccagc
      301 gagaaccctg gcggcagcaa cccacggttg gcgaggtcgt acaccgcagg catcagcttc
      361 ttgcgggaca ggtcgcctgt gacgccgaag atgacgaggc cgcaaggccc cgcgatgcgc
      421 ggcagccgcc ggtcctcggg atcccgtaat gggttgacgg aaccggacgt ctccagtgtc
      481 gtcatcgcaa cagctcctgc agggcggtca cgccggtcgc cggatcgttc aggtgcagtc
      541 gcagcaccgg ccgaccgtgg tcggcgagca cgctggcgtc accggcggcc tgtgcggcga
      601 tgagcgtccc gaacgagaac ggtcggtccg ggatgtcgat gtcggtgggc tccgtcgacg
      661 tgatctgcag gaagactccc tgcccgggcc cgcccttgtg gtactgcccg gtcgagtgca
      721 ggaagcgcgg cccccagccg aacgtcgtcg gccggccggt acgccggctg agcgtgtcac
      781 ggacgccgtg caaggccgca tcacggtcgg agtccaggta cgccatcacg gccagatagc
      841 cgtccgggcc gagccgggac aacagcgact cgacggcgcc ggagatcgtg tcgacgccgt
      901 ccagcagtcc gtcggatcca cgcgcctcca cctcgccctc ggtgatctgt gccggcgact
      961 cctccggacg tgagtcgagg agaccgcgcg ccgccttctt cgcactctcc acgtcaggct
     1021 ggtcgaacgg gtcgatgcca agcaggtggc ccagcacggc cgtggcggtc tcccaggcta
     1081 ggaactgcgc gccgagcgtg ccggagaccg tcacccacgg ctcgccgacg ccgtcagggg
     1141 tggaggtggc cgcgtccggg gacaccaacg caactgcgac gacgtcggcg ggcggctgcc
     1201 gcacctcggc tgccgatgcg tcgccgaccg cgatcggcag cacccctttg ccgagcttgc
     1261 cggtcgactc cgcgatcagc tgctctgccc agtccgcgaa gcccacgatg ccggagccga
     1321 ggtcggcgat ggctgccttg tcgcggcggc cggccgccgc caaggccacg gccagcagca
     1381 gcgccgggtt gtcggggctg tcgaagcgga cgttctcggt caccgcctcc gcctcgtcga
     1441 gcacctccgc gacgtcgatc ccggcgagcc cggtcggcac gaggccgaac gcggtcatcg
     1501 cgctgtaccg gccgccgacg tgggggtcag ccaggaagac cttgcggtag ccctgctcgg
     1561 tcgccagctt ctccaggtcc gagccggggt cggtgacgac cacgaaccga gacgccgcgt
     1621 cgatccccgc cttctcgaag gctgcggcga agatgcgccg ctggctgtcg gtctcgagcg
     1681 tgcccccgga cttgctcgac acgacgacaa ccgtccgtgt caggtcggcc gagaccgccc
     1741 gctggatgac gctcgggttg gtcgagtcga ggatcgtcag cggcacgtcg tacgtccgag
     1801 agatgacctc cggcgccagc gacgagccgc ccatgccaca gagcacgacc tggtcgaggc
     1861 cttcggacca cagctgcgca cgaagcgcgt cgatctcggc gagcagcggt cgcgagctcg
     1921 tcggcaggtc gacccaggac agccggatcg ccgcctcgga ctccgcgtcc ggtccccaca
     1981 gtgtcgggtc ctgggcggcg atcttgctgg cgagctggcg ctcgaccagt gcggccacct
     2041 ggtcgtcgta cgccgtcgag gtggtgacgg tgagcgggat cttcacctct tggctccgcc
     2101 gagcgcctcc cggacgctgt cgagcagctc ctgccaggac ttctcgaact tgtcgacgcc
     2161 ctcgtcctcg atgaccttca cgacgtcctc gtaggagatg ccgagccgct cgagtccgtc
     2221 gagcaccgct tccgcgtcgt cgtagttcgt ggtgacggtg tcaccgtcga cggtggcgtg
     2281 gtcgagggct gcctcgatcg tgccttccgg catcgtgttg accgtgtcgg gtgcgatgag
     2341 ctcgtcgacg tacatcgtgt ccgggagact cttgtccttg acggacgtgg aggcccacag
     2401 cggccgctgc ttgttggctc cggccgcttc cagcgcactc cagcgatccg aggtgaacac
     2461 ctcgtcgtac tcccggtagg caagccgggc gttcgccacc gcggccttgc tgcgcagcgc
     2521 cttggcctgc tcgacgcgtt ggccggactc gatgatcgcg tcgagccgct tgtcgacctc
     2581 ggtgtcgacc cggctgacga agaacgacgc aaccgaccgg atcgtcgaca ggtcgacgcc
     2641 cttggagtgc gcctgctcga ggccggacag gtaggcgtcc atcacggccc ggtagcgagc
     2701 caggctgaag atcaacgtca cgttgacaca gatgccctcg ctggtggcgg tggtgatcgc
     2761 cgccaggccc tcctccgtgg ccgggatctt gatcatcacg ttggagcggt cgaccagcca
     2821 ccacagcgcg cgcgcctcgg cgatcgtcgc gtccgtcttg tgcgcgagcc tggggtcgac
     2881 ctcgatgctc gcccgcccgt cctgcccgtc ggtgcggtcg taggcgtcgc gcagcacgtc
     2941 acaggcccag cgcacgtcgt acgtcgtgat ggcccggacc gcctcttcga gctcgacacc
     3001 acgcacggcg aggtccttca cctgctcggc gtagtcgtcc gcgtcgctca gcgccttcgc
     3061 gaagatggtc gggttggacg tcaccccgac gacgtggcgc tcggcgacga gccgctcgag
     3121 gttgccggtg tgcaggcgtt cgcgtgagag atcgtcgagc cagaccgaca cgccttggtc
     3181 ggagagtgct tgcagtcggt cgttcatgct ctgcttcctt tcgctcgatc tcgctgcatc
     3241 gtgggctgcg cttcggccgg cgcaggcgag tccggtcagc ctttggcgtc cgccgccgcg
     3301 atgctgtcct tggctgcctg ggtgacgacc tcggacgtca acccgaactc catgaaaagt
     3361 cgctggtagt ccgccgaggc gccgaaatgg tcgatgccga ccatccgacc cgcgtcgccg
     3421 acgatgtcgc gccaccccaa cggcaccgcc gcctccaccg acacccgagc cttgacggtc
     3481 ggcggtatca cgctgtcgcg gtacgccttt tcctgctcct cgaaccattc tcgacacggc
     3541 atcgacacga ccctggcgcg gatgccgtca gcggccagct gctcgcgcgc cgccaccgcg
     3601 atctggacct ccgagccggt accgatgagg acgacatcgg gtgcaccacc gtcgacgtcg
     3661 aggaggacgt aggcaccgcg agacacgttg gacgtgtcgg cgtacccgtc cgtgccgcgc
     3721 gggaacgtcg ggacgttctg tcgggtcagg gccaggccgg ccggccggtc ggtgtgctcg
     3781 agaaccgtcc gccatgccgc gacggtctcg ttcgcgtccg ccggccgcac cacgtcgaga
     3841 ccgggaatcg cccgcagcgc ggccaggtgc tcgatcggct ggtgggtcgg cccgtcctca
     3901 ccgagcccga tcgagtcgtg cgtccagacg tagatgaccg gcagcttcat gagtgcggcg
     3961 agccggacgg cgggccgcat gtagtcggag aagatgagga aggtcccgcc gtacggtcgg
     4021 gtgccgccgt gcagggcgat gccgttgagg agcgagccca tcgcgtgctc gcggatcccg
     4081 aagtggagaa cgcggccgta cgggtcgccg gagaacatct tcgtcgcgtg ctcggacggg
     4141 atgaagctcg gctcgccctt cggggtcgtg ttgttggagc cggccaggtc ggccgagccg
     4201 ccccacagct cgggcagccg cggagcgatg gccgacagga tctcgccgga cgccttgcgg
     4261 gtggcgactc ccttggcatc tgcgtcgaac gtgggcaacg cgtccgtcca gcccgtcgga
     4321 agcgtgcgcg tctgcatgcg ctcgaagagt tctttgcggt caccggcctt cgcggcccac
     4381 gcgtcgaacc gctcctgcca ctccgcctgc tcggctttgc cccgctcgat cgcctgccgc
     4441 gtgtggctga tgacgtcgtc ggcgacctcg aacgtcttgt ccgggtcgaa gccgaggacc
     4501 ttcttggtcg ctgcgacctc ctcgtcgccg agcgcactgc cgtgcgcctc gccggtgttc
     4561 tgcgcgttcg gtgcaggcca ggcgatgatg gtgcggagcc ggatgaagct gggccgcctc
     4621 gtctccgcct tggcgcgctt gaaggcctgg aacagtcctc ggacgttctc ctggtaggcg
     4681 gtggcgtcgt tcgtccagtc gatggactgg acgtgccagc cgtacgccgc gtagcgcttc
     4741 gcgacgtcct cgctgaacga gatcgcggtg tcgtcctcga tcgaga
//
LOCUS       DATBFZ010000462         6771 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_5413722, whole genome shotgun sequence.
ACCESSION   DATBFZ010000462 DATBFZ010000000
VERSION     DATBFZ010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 6771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6771
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5413722"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1016
                     /locus_tag="VH419_14125"
     CDS             <1..1016
                     /locus_tag="VH419_14125"
                     /inference="COORDINATES: protein motif:HMM:NF015083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peroxidase family protein"
                     /protein_id="HEY7044802.1"
                     /translation="MTTVFMREHNAICDRLHAAYPSWSDDQLFDKARLITAALIAKIH
                     TIEWTPAILGHPTLQVGMRANWFGLAGEHVHKLWGRLGGSEVLSGIPGSETNHHAAPY
                     SITEDFVTVYRMHPLIPDDYVIRGLTGAEEEQHEFMEIHGLHSHELLERVPVADALFS
                     LGTANPGAITLHNHPRFMHRLMRTDDIPIDLATVDILRSRERGVPRYNEFRRLLRLNP
                     ATSFDEIAAGDPETAEQLRAVYGDDIEKVDTTVGMFGERLPKGFGFSDTAFRIFVLMA
                     SRRLKSDRFYTVDFTPRMYTPEGMAWLDDNDMISVLLRHYPELAPALRTQRNAFAPWP
                     RPS"
     gene            complement(1130..2680)
                     /locus_tag="VH419_14130"
     CDS             complement(1130..2680)
                     /locus_tag="VH419_14130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012922191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY7044803.1"
                     /translation="MSDYGHEVEFGAFITPNADAIEQILELAQLAEVVGLDVVSVQDH
                     PYQAKFVDTWTLLSVLAARTSAVTLAPNVASLPLRPPVVLAKSAATLDLLTGGRLALG
                     LGAGSFWDAIEAAGGPRRTPKQAVDAMVEAITLIREFWSGGSLRFIGEHYRAKGLHAG
                     PTPAHDIPIWVGAYGPRMLRITGELADGWVPSMGYADPGRLAELNERVDEAAEAAGRS
                     PQAIRRIYNVFGRFGSDSGFLQGTPMDWSEQLAGLTLEQGISTYILGTDDADTLRRFA
                     DEVVPAVRELVAAERSRPADDEQPAPITAHAWSEAAPLSVQATSDTGERRTGELPWDE
                     PSRPTHAEPVNAAYTSDQQAVPQHLVDIHDALRAELQQVRAIVAQVRNGHLTVGAARS
                     AINTMTMRQNNWTLGAYCESYCRIVTGHHTLEDRSIFPHLRRSETALGQVLDRLEMEH
                     KVIAGVLDDVDRALIALVTGDNAGVAGRESLEELQHQVDLLTDTMLSHLAYEERELIG
                     PLARHGFT"
     gene            2823..3923
                     /locus_tag="VH419_14135"
     CDS             2823..3923
                     /locus_tag="VH419_14135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance/C4-dicarboxylate
                     transporter family protein"
                     /protein_id="HEY7044804.1"
                     /translation="MTDGIGQRIAVAVRGLTPGYFALVMATGILSVGMRLEDVDPISE
                     SLLVVCAAAFVVLLGLTAWRFVAFRSAVVEDFTDPRRGFGFFTFVAGTNVLGVRLGLE
                     GAYAATAVLLALAGLAWLSLGYVVPWTAVLGRQERPVVATANGTWFIWVVASQSVAVA
                     AATIEPVYESARRELAVLAIVSWSVGVFLYAAAAVLVSLRLMLYEFGPDDLTPPYWVS
                     MGALAITVLAGARIVEMADAPMVTVTRDLIAGLAVVFWAFATWLIPVLVAAGWWRHVR
                     RGVPLVYGATLWSLVFPLGMYAVAGIYLGHADRLPVVQAIGKAELWVAFAVWLVVFVA
                     MARHVVTTVLRAGERSAARDAGQTRAMSTKRD"
     gene            complement(3999..5270)
                     /locus_tag="VH419_14140"
     CDS             complement(3999..5270)
                     /locus_tag="VH419_14140"
                     /inference="COORDINATES: protein motif:HMM:NF022432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HEY7044805.1"
                     /translation="MGRNSKARRDARKRNQTRQGHRPKGPTDSPFGFGDGPNMDPLPE
                     AELHVVTQVRRLGPRGSEADARSRAQYLRRLGFPSWTLRRAVRGVLERVVATALRGGW
                     THAELLEAVDRVVGPGHRSVIAGLVLSVDPAGNNAFDGNTEGMTVEPPRNLKLADDAE
                     LALALRLASLLAALPATVLPNRRRTPPGTSNGSRAAAGKLAQVRALLAKAEATTFPEE
                     AETLSAKAQELISRHSLEELLVHDHQADDDRSSMTYRRLWLDAPYVDAKASLVDQVAR
                     TNRCRSVYDPRLGFCTVVGSPFDLDALDLMVTSLLAQAQRAMLAHGSRIDVIGRSRTR
                     SFRQSFLVSFAVHIGQRLQRAADDSVIECGGSSLLPAIRDHEVEVDELTTEMFPHVVS
                     KGAAISNAEGWAGGRAAAELARLSIFDEVNS"
     gene            complement(5337..6707)
                     /locus_tag="VH419_14145"
     CDS             complement(5337..6707)
                     /locus_tag="VH419_14145"
                     /inference="COORDINATES: protein motif:HMM:NF026240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LAGLIDADG family homing endonuclease"
                     /protein_id="HEY7044806.1"
                     /translation="MWSLDDRLKLVPRTMTHAFASGIKEVFGVKLASGREIKATSNHP
                     FLTCEGWRALGDLRPGSRVGVSRHVPPPLMTTAMEDDELILLGHLIGAGSFVRRQPIR
                     YASVDEENLAVVAAAANRRFGVDAVRDDRPAARVTTLRLPAPFHLSHGRRSPIAAWLD
                     GMGLFGCRSHERFVPAPVFSVPKRQVAVFLRHLWATDGSVTWDPTARSGCVSFASTSR
                     RLVDDVARLLLRFNVLSRMQRVTKPGCRDTFHLHIDGGDDQRRFLQEVGLHGARGTLA
                     AEVLTKIGRAKADTDLDTIPQDVWDRVRTALVERPVTHREFASAVGNEFGGSTLWRQA
                     PNRPRLAKIAAALDAADLEVLATNDIFWDEIRKVESLGEQQVYDATVLGTHNFIADGI
                     ALHNSLEQDADMVILLHRDDIYEKEPTRPGEADIIVAKHRNGPTADLVLAFQGHYSRF
                     VDMARA"
BASE COUNT         1033 a         2273 c         2299 g         1166 t
ORIGIN      
        1 tgatgacgac cgtgttcatg cgtgagcaca acgcgatctg cgaccggttg cacgcggcat
       61 acccgagctg gtcggacgac cagctcttcg acaaggcccg cctcatcact gcggcgctga
      121 tcgcgaagat ccacacgatc gagtggacgc cggcgatcct cggccacccg acgttgcagg
      181 tcggcatgcg ggccaactgg ttcggcctcg ccggcgagca tgtgcacaag ctgtgggggc
      241 ggctcggtgg gagcgaggtg ctcagcggca tacccggctc ggagaccaac catcacgccg
      301 cgccgtactc gatcaccgag gacttcgtga cggtctatcg gatgcacccg ctgatcccgg
      361 acgactacgt catcaggggg ctcaccggag cggaggagga gcagcacgag ttcatggaga
      421 tccacgggct ccactcacac gagctgctcg agcgggtccc ggtcgccgac gcgctgttct
      481 cgctcggcac ggccaacccc ggcgcgatca cgttgcacaa ccacccgcgg ttcatgcacc
      541 ggctgatgcg taccgacgac atcccgatcg acctcgccac cgtcgacatc ctgcggtcgc
      601 gcgaacgagg cgtgccgcgc tacaacgagt tccgccggct gctccggttg aaccccgcaa
      661 cgtcgttcga cgagatcgcg gccggcgatc cggagaccgc tgagcagctg cgtgcggtgt
      721 atggcgacga catcgagaag gtggacacca ccgtcgggat gttcggcgag cgactgccga
      781 aggggttcgg tttcagcgac acggccttcc ggatcttcgt gctgatggcg tcgcggcgac
      841 tcaagagcga ccgcttctac acggtggact tcacgccccg catgtacacc ccggaaggca
      901 tggcctggct ggacgacaac gacatgatct cggtgctgct gcggcactac ccggagctcg
      961 ccccggctct gcggacgcag cgcaacgcct tcgccccctg gccccgcccc tcctgatgcg
     1021 atgacccgtg acctggaccg tccgctcggg ctcaccgcga attgcgggaa agtggcggtc
     1081 ggcgcgctgc ctcacccgtg acttttcccg caattcgcgg gagccagggt caggtgaagc
     1141 cgtgccgcgc caacgggccg atcagctcgc gctcctcgta ggcgaggtgg gacagcatcg
     1201 tgtcggtcag caggtcgacc tggtgctgca actcctcgag cgactcccga cccgcgacgc
     1261 cggcgttgtc gccggtcacc agcgcgatga gggcacggtc gacgtcgtcg agcaccccgg
     1321 cgatgacctt gtgttccatc tccagccggt ccaggacttg gccgagggcg gtctcggagc
     1381 gacgcaggtg cgggaagatg ctgcggtcct ccagcgtgtg atggccggtg acgatccggc
     1441 agtaggactc gcagtacgca ccgagtgtcc agttgttctg ccgcatggtc atcgtgttga
     1501 tcgccgagcg cgccgcaccg accgtgaggt ggccgttgcg gacctgcgcg acgattgcgc
     1561 gaacctgctg gagctcggcg cgcagcgcgt cgtggatgtc aacaagatgc tgcggcactg
     1621 cctgctggtc cgacgtgtac gccgcattga ccggctccgc atgcgtggga cgactcggct
     1681 cgtcccaggg gagctccccg gtgcggcgtt cacctgtgtc gctcgttgcc tgaaccgaga
     1741 gtggggcggc ttccgaccat gcgtgagcag tgatcggcgc aggctgttca tcatccgctg
     1801 gccgggagcg ctccgccgcc accagctccc ggaccgccgg cacgacttcg tcagcgaacc
     1861 gacgcaacgt gtcagcgtcg tccgtgccga ggatgtacgt gctgatcccc tgctcgagcg
     1921 tcagacctgc cagttgttcg ctccagtcca tcggcgtgcc ctgcaagaac ccgctgtcgc
     1981 tgccgaaccg gccgaagacg ttgtagatgc gcctgatcgc ctgaggtgag cgtccggcgg
     2041 cctcggctgc ctcgtcgacg cgctcgttga gctcagcgag ccggcccggg tcggcgtacc
     2101 ccatgctcgg tacccaaccg tcggccagct caccggtgat ccgcagcatc cgcggaccat
     2161 aggcgcctac ccagatcggg atgtcgtgag ccggtgtcgg ccctgcatgc agtcccttgg
     2221 cgcggtagtg ctcgccgatg aagcgcagcg accctcccga ccagaactct cgtatcagcg
     2281 tgatcgcttc gaccatcgca tcgaccgctt gtttcggcgt acgccgcgga ccgccggcgg
     2341 cctcgatcgc gtcccagaac gaccctgcgc cgagtccaag cgccagccgc cccccggtga
     2401 ggaggtcgag ggtggcggcc gacttggcca gcaccacggg gggacgcagc ggcaggctgg
     2461 cgacgttcgg cgccagcgtg accgcgctgg ttcgtgcggc cagcaccgac agcaatgtcc
     2521 aggtgtcgac gaacttcgcc tggtacggat ggtcctgcac cgagacgacg tcgagaccca
     2581 ccacctccgc gagctgcgcc agctcgagga tctgctcgat cgcgtcggcg ttcggcgtga
     2641 tgaacgcgcc gaactcgacc tcgtgtccgt agtcgctcat ccggatggct ccgcaggcgg
     2701 ccttcccacg tcgtgacctc caatcagcaa cccggtgacc tcggctggtg ccagacccta
     2761 ctggtagacg cgtgtcgtgt gagccctcga ccgcagcacg caaggtacgg gaccatcggc
     2821 gtgtgactga cggcatcggg caacggatcg ccgtcgcggt tcggggtctg acgcccggct
     2881 acttcgcgct ggtgatggcg accggcatcc tctcggtggg catgcggctg gaggacgtgg
     2941 acccgatctc ggagtcgctg ctcgtggtgt gcgccgcggc cttcgtcgtg ctgctcggac
     3001 tgacggcctg gcggttcgtc gcgttccgaa gcgccgtcgt ggaggacttc accgaccccc
     3061 gccgaggctt cgggttcttc acgttcgtag cagggaccaa cgtcctcggc gtccggctcg
     3121 gtctggaagg cgcctacgcc gccactgcgg tgctgctcgc gctggccggt ctcgcctggt
     3181 tgtcgctcgg gtacgtcgtc ccgtggacgg ctgtcctggg ccggcaggag cggcccgtcg
     3241 tggccaccgc gaacggcacc tggttcatct gggtggtggc gagccagtcg gttgcggtcg
     3301 cggcggcgac catcgaaccg gtctacgagt ccgcccgacg ggagctggcg gtacttgcga
     3361 tcgtctcctg gtcggtgggg gtgttcctgt acgccgcggc agcggtcttg gtgtcgctgc
     3421 ggctgatgct gtacgagttc ggccctgacg acctgacgcc gccgtactgg gtctccatgg
     3481 gcgcgctggc catcacggtg ctcgccggtg ccaggatcgt ggagatggct gacgcgccga
     3541 tggtgacggt cactcgggac ctgatcgccg gcctcgcggt ggtgttctgg gcgttcgcga
     3601 cctggctcat cccggtgctc gttgctgcgg gttggtggcg acacgtgcgt cgcggggttc
     3661 cgctggtgta cggggccacg ctgtggagcc tggtgttccc cctcggcatg tacgccgtcg
     3721 cgggcatcta cctgggtcac gcggaccggt tgcccgttgt ccaggccatc ggcaaggccg
     3781 agctgtgggt ggccttcgcc gtctggctcg tggtgttcgt cgccatggcc cgccacgtcg
     3841 tcaccacggt gctgcgagcc ggtgaacggt ccgcagcgag ggacgccggt cagacgcggg
     3901 ccatgtcgac gaagcgggac tagcggtcct ggaatccgcg ccaggtcaac cgccagctgg
     3961 tgtcgactcg aggatgtgtt cggtgtgctg acgcccggtc aggagttgac ctcgtcgaag
     4021 atgctgaggc gggcaagctc ggcggcggca cgaccacctg cccaaccctc ggcgttcgag
     4081 atggcggcac ccttggagac gacatgcggg aacatctcgg tggtgagctc gtcgacctcg
     4141 acctcgtgat cacggatcgc cgggaggagg gatgaccctc cacactcgat gacggagtca
     4201 tcggccgccc gctgcaaccg ctgaccgatg tggaccgcga acgaaaccag gaacgactgg
     4261 cggaacgaac gggtgcgcga ccggccgatc acgtcgatgc gtgacccgtg ggcgagcatg
     4321 gcccgctgag cctgggccag cagcgaggtg accatcagat cgagcgcgtc gaggtcgaac
     4381 ggcgacccca ccaccgtgca gaagccgagc cgagggtcgt acaccgatcg acatcggttc
     4441 gtccgtgcga cctggtcgac gaggcttgcc ttggcgtcga cgtatggcgc gtcgagccac
     4501 agccgccgat acgtcatgct cgaccggtcg tcgtcggcct gatggtcgtg aaccagcagc
     4561 tcctccaacg agtgcctgct gatcagctcc tgcgccttgg cggacagcgt ctccgcctcc
     4621 tcggggaacg tcgttgcctc ggccttggcc aacagcgctc gtacctgtgc gagctttccc
     4681 gccgccgccc gcgaaccgtt cgacgtcccg ggcggcgtcc tacgtcggtt gggcagcacg
     4741 gtcgcaggca aggcggccag cagcgatgcc agccggagcg ccagagcgag ctcggcatcg
     4801 tccgccagtt tcagattcct gggcggttcg acggtcatgc cctcggtgtt gccgtcgaac
     4861 gcgttgttgc cggccgggtc taccgaaagc accagacccg cgatcactga gcgatggccc
     4921 ggcccgacga ctcggtcgac cgcctccagc agctctgcat gagtccagcc gccacgcaga
     4981 gcagtcgcga cgacgcgttc gagcacgcct ctgaccgccc ttcggagcgt ccatgagggg
     5041 aacccgagcc gccggaggta ctgcgccctg ctgcgagcgt ccgcctcaga gccgcgcggg
     5101 cccagcctgc gcacctgggt gaccacgtgg agctcggcct ccggcagcgg atccatgttc
     5161 ggcccgtcgc cgaacccgaa tggcgagtcg gtcggacctt tcggtcgatg gccttgccgt
     5221 gtctgattcc gcttgcgggc gtcacgccgt gccttgctgt tgcggcccat cggggggctc
     5281 cttcctcgtg cttcgaacgg tgcgaccccc agatcggtgt gacatgacgg cggctgtcag
     5341 gcgcgggcca tgtcgacgaa gcgtgaatag tggccctgga aggcgagcac gaggtcggcg
     5401 gtggggccat ttcggtgctt ggcgacgatg atgtccgcct cccccggccg agtgggttcc
     5461 ttctcgtaga tgtcgtcgcg gtgcagcaag atcaccatgt cggcgtcctg ctcgagggaa
     5521 ttgtgcaggg caatgccgtc ggcgatgaag ttgtgcgtgc caaggaccgt cgcgtcgtac
     5581 acctgctgtt cgccgaggct ctcgaccttg cggatctcgt cccagaagat gtcattcgtt
     5641 gcgaggacct cgaggtcggc ggcatcgaga gcagcggcga tcttggccaa ccgtggacgg
     5701 ttcggcgcct gcctccacag ggtcgaacca ccgaactcgt tgcccaccgc tgacgcgaac
     5761 tcgcgatgcg tcaccggtcg ctcgacgagc gcggtgcgta cgcggtccca cacgtcttgc
     5821 ggaatcgtgt cgagatcggt gtcggccttg gcacgaccga tcttcgtgag cacttcggct
     5881 gcgagcgtcc cgcgtgcgcc gtgaaggccg acctcctgca ggaaacggcg ttggtcgtct
     5941 ccgccgtcga tgtgcaggtg gaacgtgtcc ctgcaccccg gcttcgtgac tcgctgcatc
     6001 ctgctcagca cgttgaatcg gagcagcagt ctcgcgacgt cgtcgacgag acgtcggctt
     6061 gtcgatgcga aggagacgca gccgctgcgg gcggttgggt cccacgtcac ggacccgtcg
     6121 gtcgcccaaa ggtgtcgaag gaacacggca acctggcgct tgggaacgct gaacactgga
     6181 gcaggcacga atctctcgtg gctgcgacaa ccgaacagcc ccattccgtc caaccacgcc
     6241 gcgatgggac tgcgacgacc atgactgagg tggaagggcg cggggagccg cagcgttgtc
     6301 acgcgagccg ccggacggtc gtctcgaacg gcgtcaacgc cgaaccgacg gttcgccgcg
     6361 gcagcaacga ccgcgaggtt ctcctcgtcg acgctggcgt atcgaatcgg ctggcgccgc
     6421 acgaacgaac cggcaccgat caggtgaccg agcaggatca actcgtcgtc ttccatcgcc
     6481 gtcgtcatca acgggggagg gacgtgtcga ctgacgccga cccgcgatcc agggcgcaga
     6541 tcgccgagtg cccgccaccc ttcacaggtg aggaacggat ggttcgacgt cgccttgatc
     6601 tctcgacctg atgcaagctt gacgccgaac acctccttga tgccgctggc gaacgcgtgc
     6661 gtcatggtgc gcggcaccag cttcaggcgg tcgtccaagg accacacggg gatctcggtc
     6721 gcgccagtgg cgaccaactc tccgagcgag acatcgacgt tcgtgtcggc c
//
LOCUS       DATBFZ010000463         4966 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_2948216, whole genome shotgun sequence.
ACCESSION   DATBFZ010000463 DATBFZ010000000
VERSION     DATBFZ010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 4966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4966
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2948216"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..726
                     /locus_tag="VH419_14150"
     CDS             <1..726
                     /locus_tag="VH419_14150"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY7044807.1"
                     /translation="VPLNLDSEVTVVLTIDVSLSMSAADVDPDRITAAKETAKEFIDK
                     LPSNFKVGIVSFSKVATVDMPPTKDHVAAENAVDALTLSEYTATGEGIYSALAVIEQD
                     LAATGQEDADKPPAFIVLISDGARTIGRSQVGAAEAAKADRIPIYTVALGTLNATIES
                     DGKQIPVPVEIPQLEEVARISGGKSYVAESPNDLVKAYSSVDSQIRYVTERGDATSEY
                     MGVLVLLVLLSTGAGLSVAARWP"
     gene            865..1398
                     /locus_tag="VH419_14155"
     CDS             865..1398
                     /locus_tag="VH419_14155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3145 domain-containing protein"
                     /protein_id="HEY7044808.1"
                     /translation="MNRSLHNETGSAATTARGVLYVHASPSALCPHVEWAVGGALAVA
                     VDLAWTAQPAKVGAYRAEYSWTGPVGSGARIASALRGWEHLLFEVTEDATSVSEGARY
                     SFTPELGLFHAVTGLHGDIMIPEDRLRAACVKASMGAVTLVDEIDKLLGKAWDDSLEP
                     FRHAGDGAPVRWLHHVV"
     gene            1901..2062
                     /locus_tag="VH419_14160"
     CDS             1901..2062
                     /locus_tag="VH419_14160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044809.1"
                     /translation="MTDPMTGFQVAKLPKDWRAGDWERNQRLIQAATDEHNESGDAWC
                     SLRWVPTNG"
     gene            complement(2221..3687)
                     /locus_tag="VH419_14165"
     CDS             complement(2221..3687)
                     /locus_tag="VH419_14165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008364248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEY7044810.1"
                     /translation="MTTTDNAPVQAPEQAAPPQQAKPPKLPREQDPRNPNKRLAALFD
                     EGSLELISPDDDSGMLAAVGTVDGVRTVAYCSDATIMGGAMGNDGCKVVVDAYQRAMA
                     DQAPIVGLWHSGGARLAEGVLSLHAVGQIFHAMTQASGKIPQISVVLGPAAGGAAYGP
                     ALTDVVILGPEGRIFVTGPDVVRSVTGEDVDMLRLGGPEPHGRRSGVVHVLAESEAHA
                     LGEARRLVALLGDQGTIDPEHVKDVELSDELPESKKRAYDVHPLVHELLDGDSMLELH
                     NKWAPNIVTAFGRFGGRTVGVIANNPLRLGGCLDSPSAEKAARFVRMCDAYGVPLIVV
                     VDVPGYLPGVGQEWDGVVRRGAKLLHAFSEAQVPRVTLVTRKAYGGAYIAMNSRSLGA
                     TKVFAWPGAEIAVMGAVAAVRILHRRKIAAVAADLRPTVETELAAEHERIAGGVDKAV
                     EIGVVDEVVEPGRTRSALAAALFDADTGSRGAHGNIPL"
     gene            complement(3712..4950)
                     /gene="fabF"
                     /locus_tag="VH419_14170"
     CDS             complement(3712..4950)
                     /gene="fabF"
                     /locus_tag="VH419_14170"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020385813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEY7044811.1"
                     /translation="MTSQRVVVTGLGATSPVGGDVPSTWDALLAGTSGARLLTEDWAE
                     QLPVRIAARVAVEPGEVLERVKARRLDRSGQFALIAALEAWGDAGLGDADVDTERLGV
                     AMASGIGGVTTLLNNYDVLLEKGPRRVSPLAIPMLMPNSPAANIGLAIGARAGVHTPV
                     SACASGNEAIAHGIDMIRLGRADVVVVGGTEAAIHPLPMAAFGNMMALSKRNDEPEKA
                     SRPWDVDRDGFLLGEGSAALVIESAEHAERRGAKVYAEVAGAGVTADSHDIAQPDPSG
                     SGATRAMAMALEVGHLSPRQVVHINAHATSTPQGDIAECVAITNALGGETDHVVVTST
                     KSMTGHLLGAAGALESVAAVLALYNRVVPPTINVDNLDPAVKLDVATEQRKLPDGEIC
                     ALNNSFGFGGHNVAIAFRSV"
BASE COUNT          793 a         1650 c         1713 g          810 t
ORIGIN      
        1 gtgccgctca acctcgacag cgaggtcacg gtcgtcttga ccatcgacgt gtcactttcg
       61 atgtctgccg ccgatgtcga cccggaccgc atcacggcgg cgaaggagac cgcgaaggaa
      121 ttcatcgaca agctgccgtc gaacttcaag gtcggcatcg tcagcttctc gaaggtggcc
      181 acggtcgaca tgccgccgac caaggaccac gtcgccgccg agaacgccgt cgatgcgttg
      241 acgctgtcgg agtacaccgc gaccggtgag ggcatctaca gcgcgctggc cgtgatcgag
      301 caggacctgg cggcgacggg ccaggaggat gccgacaagc cgccggcgtt catcgtgttg
      361 atcagcgacg gcgcgcggac gatcggccgt tcgcaggtcg gtgctgctga ggcagcgaag
      421 gcggaccgga ttcccatcta cacggtggcg ctggggacgc tcaacgcgac catcgagtcg
      481 gacggcaagc agatcccggt gccggtcgag attccgcagc tggaggaggt ggctcggatc
      541 agtggcggga agtcctacgt cgccgagtcg cccaacgacc tggtgaaggc ctactcgtcg
      601 gtggacagcc agatccgcta tgtcaccgag cgcggcgatg ccacgtcgga gtacatgggt
      661 gttctcgtgc tgctggtcct gctgtcgacg ggcgcggggt tgagcgtcgc tgctcgctgg
      721 ccgtgacggg tacggcgcgc tgacctgggg ccgaatcctt ggtgcagatg tggcggatga
      781 tgcacaatag agccggagtt cacgactgat cctcgtggta cgagccagct ggggaaggcg
      841 acgctcgagc cttgaggagg atcggtgaac cgcagcctgc acaacgagac gggctccgcg
      901 gccacgacgg cgcgcggagt cctctacgtc cacgcgtcgc cgtcggcgct gtgcccgcac
      961 gtcgagtggg cggtcggcgg tgccctcgcg gtggccgtcg acctggcctg gacggcgcag
     1021 cctgcgaagg taggcgcgta ccgtgcggag tactcgtgga ctggtccggt gggctcgggt
     1081 gcccggatcg cgtcggcgct gcgtggctgg gagcacctgc tgttcgaggt gaccgaggac
     1141 gcgacatcgg tgagcgaagg cgcccgctac tcgttcacgc cggagctcgg gttgttccat
     1201 gcggtgacgg ggctgcacgg cgacatcatg atcccggagg accggctcag ggcagcctgc
     1261 gtcaaggcct cgatgggggc cgtgacgctg gtggacgaga tcgacaagct gctcggcaag
     1321 gcctgggacg actcactcga gcccttccgc cacgccggcg acggcgcccc cgtccgctgg
     1381 ctccaccacg tcgtctgacc ttctcgttga accgtgagtt gtggttcgtt gagccgtgag
     1441 ttgtggttcg ttgagtcgtg ggttgtggtt cgttgagtcg tgggttgtgg ttcgacgaag
     1501 ggcgaccgcg tcttgctagg ggttcgtgcc gcagttgcag tccaaccacg ggtacccctt
     1561 ccgtccggcc tcgccgcggg aatgttgcgg agtaccgttg cgatagtcct ggcaggagct
     1621 accgggcggc gcgacgaccc gggagccggt ggttggcgga gtggggatct gaccaatcct
     1681 gatcccgaca tcccaacggc gatggatcgg agcacttctg aaccagcggg tcagcacggg
     1741 ttcccaccac acggtcaccg gcaaacggat cgtggccggt ggggcgatcc tggtggtgac
     1801 gttgggcttg gtggtactga tcgccgacca cgaggagcca cttcccagga cctgcgcggg
     1861 catcaacgcg gagcgagacc agatcctcga acggaacacg atgaccgacc cgatgaccgg
     1921 atttcaagtc gccaagttgc cgaaggattg gcgtgctggc gactgggaac gcaaccagcg
     1981 gttgatccag gctgccaccg acgagcacaa cgaaagcggc gatgcgtggt gctcgctccg
     2041 gtgggtgccg accaatggct gatgtgcaga agtcaggtgg agatttcggt tcagcgcagg
     2101 gtcagcgccg gcgcgcagtt gacagttcct ctcgtcacgg ctctcgatga acacgaactc
     2161 acggttcgac gaaccacaac tcacggttca acgaaccaca actcacggtt caacgagggg
     2221 ttagaggggg atgttgccgt gggcgcctcg ggagccggtg tcggcgtcga agagggcggc
     2281 ggccagcgcg gaacgggttc gtcccggttc gacgacctcg tcaaccacgc cgatctcgac
     2341 ggccttgtcg acgcctccgg cgatcctctc gtgctcggcc gccaactcgg tctcaaccgt
     2401 cggccgcagg tccgcagcga ccgcggcgat cttgcgacga tgcagaatgc gcaccgcggc
     2461 caccgcaccc atcacggcga tctcggcacc cggccacgcg aacaccttcg tcgccccgag
     2521 cgagcgtgag ttcatcgcga tgtacgcccc gccgtacgcc ttccgcgtca cgagcgtcac
     2581 ccgcgggacc tgcgcctcgc tgaacgcgtg cagcagcttc gcaccccggc gtacgacgcc
     2641 gtcccactcc tggccgacac ccggcaggta gccaggcaca tcgaccacga cgatcagcgg
     2701 tacgccgtac gcgtcacaca tccgcacgaa ccgcgcggcc ttctccgcgg acggcgagtc
     2761 cagacaaccg ccgagccgca gcgggttgtt cgcgatcacg ccgaccgtac ggccgccgaa
     2821 ccgtccgaac gcggtcacga tgttcggcgc ccacttgttg tgcagctcga gcatcgagtc
     2881 gccgtccagc aactcgtgca cgagcgggtg cacgtcgtac gcgcgcttct tcgactcggg
     2941 cagctcgtcg gagagttcga cgtccttgac gtgttccggg tcgatcgtcc cctggtcacc
     3001 caacagcgcc acgagccggc gcgcctcacc cagcgcatgc gcctcggact cggccaacac
     3061 gtggaccacg ccggaacgcc gaccgtgcgg ctccggacca ccgagccgga gcatgtccac
     3121 gtcctcgccg gtcaccgacc gtacgacgtc tgggcccgtc acgaagattc gtccctccgg
     3181 gccgaggatc accacgtcgg tcagcgccgg accgtatgcc gcaccaccag cggccgggcc
     3241 gagcaccacc gagatctgcg ggatctttcc ggaggcctgc gtcatggcgt ggaagatctg
     3301 acccacagcg tgcagcgaca acacgccttc ggcgagccgt gcgccgccgg agtgccagag
     3361 cccgacgatc ggagcctggt ccgccatcgc gcgctggtag gcgtccacca cgaccttgca
     3421 gccgtcgttg cccatggcac cacccatgat cgtcgcgtcc gagcagtacg ccaccgtgcg
     3481 tacgccgtcg acggtgccga cagctgcgag catgcccgag tcgtcgtcgg ggctgatgag
     3541 ctcgaggctg ccctcgtcga acagcgccgc caaccgcttg ttcgggttgc gcgggtcctg
     3601 ctcgcggggg agcttcggcg gtttcgcctg ctgcgggggg gcagcctgct cgggtgcctg
     3661 caccggtgcg ttgtcagtcg tggtcatggg gaccccttaa gggagcaagg ctcagacgga
     3721 gcggaaggcg atggcgacgt tgtggccgcc gaagccgaac gagttgttga gcgcgcagat
     3781 ctcgccgtcg ggcagcttgc gctgctcggt ggcgacatcg agcttgacgg cgggatcgag
     3841 gttgtcgacg ttgatggtcg gtggtacgac gcggttgtac agcgcgagca cagcggccac
     3901 cgactccagc gcaccggcag cacccagcag gtgaccagtc atcgacttgg tcgacgtgac
     3961 gacgacatgg tcggtctcac cgccgagggc gttcgtgatc gcgacgcact cggcgatgtc
     4021 gccctgcggc gtggacgtgg cgtgcgcatt gatgtgcacc acctgtcgcg gactcaggtg
     4081 gccgacttcc agggccatgg ccatggctcg tgtcgctccc gaacctgacg ggtccggttg
     4141 ggcgatgtcg tgcgagtcgg ctgtgacgcc tgcgccggcc acctcggcgt acaccttcgc
     4201 gccccgtcgt tcggcatgct ccgcgctctc gatgaccagg gcggcgctgc cctcgccgag
     4261 gaggaaaccg tcgcggtcga catcccacgg tcgagacgcc ttctccggct cgtcgttgcg
     4321 cttggacagc gccatcatgt tgccgaaggc cgccatcggc agtgggtgga tggcggcctc
     4381 tgtgccgccg acgacgacca catcggctcg gcccagccgg atcatgtcga tgccgtgggc
     4441 gatcgcctcg ttgccggacg cacacgcgga gacgggcgtg tggacaccgg cccgcgcgcc
     4501 gatcgccaag ccgatgttcg cggccggact gttgggcatc agcatcggga tggccaacgg
     4561 cgagacacga cgcgggccct tctccagcag aacgtcgtag ttgttcagca gggtcgtcac
     4621 gccaccgatc ccggaagcca tcgcgacccc gagccgctcg gtgtccacgt cggcatcgcc
     4681 gaggcccgcg tcaccccacg cctcgagcgc cgcgatcagg gcgaactggc cggagcggtc
     4741 cagccggcgg gccttgaccc gctccagcac ctcaccgggt tcgacggcga cgcgtgcggc
     4801 gatccggact ggcagctgct cggcccagtc ttcggtcagg agacgggcgc cggaggttcc
     4861 ggccagcagg gcgtcccagg tgctgggcac gtctcctccg accggggacg tcgcaccgag
     4921 gccggtcacg acgacgcgct ggctcgtcat caggtaactc cttcgt
//
LOCUS       DATBFZ010000464         1631 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_323459, whole genome shotgun sequence.
ACCESSION   DATBFZ010000464 DATBFZ010000000
VERSION     DATBFZ010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 1631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1631
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323459"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..622
                     /locus_tag="VH419_14175"
     CDS             <1..622
                     /locus_tag="VH419_14175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HEY7044812.1"
                     /translation="QVFCMDEPLSNLDAKMRVQTRTDIARLQADLGVTTVYVTHDQIE
                     AMTMGDRVAVMNLGVLQQVDSPLNLYDRPANLFVAGFIGSPQMNLMEATVVDGKAKVG
                     TYDVPVDAASERRMEGNITIGVRPEAWRIVSPDQGGLPVDVTVVEELGADAFVYGTSD
                     VEGTPHNIVVRVSDRNSVHKGDVIHVTTDPRSVHVFDTDSGERLSD"
     gene            complement(663..1109)
                     /locus_tag="VH419_14180"
     CDS             complement(663..1109)
                     /locus_tag="VH419_14180"
                     /inference="COORDINATES: protein motif:HMM:NF016840.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEY7044813.1"
                     /translation="MDELEALMAALRSVRSSMLGTLAGLSEKDARRSTVPSGTNLAGL
                     VQHLTFVESTWFEQIVGGGRPTRGKRSMDVDPSVSVSSLRSDYRAACEASDAVILRVG
                     DPAARVEHNGKARDLRWAVLAVINETARHAGHADIIREQIDGRTGR"
     gene            complement(1242..>1631)
                     /locus_tag="VH419_14185"
     CDS             complement(1242..>1631)
                     /locus_tag="VH419_14185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044814.1"
                     /translation="GPPLVLVERRDLRDARADDACLSLRQSRLVLEALRPRRRLRRLA
                     GQATSSGQTFPGPPLLGVRDPGSDQQVGESLVVMVQKAVPLFCRLSGRRDRERSHGED
                     PPGRAEPHVRRSVKDFRPARIALRIEI"
BASE COUNT          275 a          568 c          545 g          243 t
ORIGIN      
        1 ccaggtgttc tgcatggacg agccgctgtc caacctcgac gccaagatgc gggtccagac
       61 gcgcaccgac atcgcccgcc tgcaggccga cctgggggtg acgacggtct acgtcacgca
      121 cgaccagatc gaggcgatga ccatgggtga ccgggtggcg gtgatgaacc tcggggtcct
      181 gcagcaggtc gactcgccgc tgaacctcta cgaccggccg gcgaacctct tcgtcgccgg
      241 gttcatcggc tcgccgcaga tgaacctgat ggaggccacc gtcgtcgacg ggaaggcgaa
      301 ggtcgggacg tacgacgtgc cggtcgacgc cgcgtccgag cgccggatgg agggcaacat
      361 caccatcggg gtccgtcccg aggcctggcg gatcgtctcg ccggaccagg gtgggctgcc
      421 cgtcgacgtc accgtggtcg aggagctcgg cgcggacgcg ttcgtgtacg gcacctccga
      481 cgtggaaggc acgccgcaca acatcgtggt ccgggtgagc gaccgcaact ccgtgcacaa
      541 gggcgacgtc atccacgtca ccaccgaccc gcgcagcgtg cacgtcttcg acaccgactc
      601 cggcgagcgc ctgtccgact gaggctccgg ccacaggaac agtcaccaca gggccgcacc
      661 gctcagcggc cggtcctgcc gtcgatctgc tcccggatga tgtcggcatg gccggcgtgg
      721 cgggcggtct cgttgatgac ggcgagcacg gcccaacgca ggtcgcgggc cttgccgttg
      781 tgctcgaccc gcgccgccgg gtcgcccacc cgcaggatca ccgcgtcgct ggcctcacag
      841 gcggcgcggt agtcgctgcg cagggacgag accgacaccg acgggtcgac gtccatcgaa
      901 cgctttccgc gtgtcggcct gccgccgccg acgatctgct cgaaccacgt cgactccacg
      961 aaggtcaggt gctgcacgag cccggcaagg ttcgtcccgc tcggcaccgt gctgcggcgc
     1021 gcgtccttct cactgagccc cgcgagcgtg cccagcatgc tcgagcgcac gctccggagg
     1081 gcggccatga gggcctcgag ctcgtccacg agcgcatcct ggcacgtgct gaccgccacg
     1141 gagaccggga gaaggaaacc cactggacga gaattgcgaa tatctgtaat gttcgagggt
     1201 ggtgatcaac tcggatattc gctgcgatta tgaccaacat atcagatttc aattcgaagt
     1261 gcaattctcg ccggtcggaa gtccttgacc gaccgcctga cgtggggttc cgcccgtccc
     1321 ggcgggtcct caccgtggct gcgttcgcgg tcacgtctgc ccgacaaccg acagaagaga
     1381 ggtaccgcct tttgaaccat gaccacgaga ctctcgccca cctgctgatc gctgcccggg
     1441 tcgcggacgc cgagcagcgg cggaccggga aacgtctggc ccgagctcgt cgcctgaccc
     1501 gccaagcggc gcaggcgtcg gcgcgggcga agcgcctcca gcaccaggcg gctctggcgc
     1561 aacgacaggc acgcgtcatc ggcgcgcgcg tcacgtagat ctcgtcgctc gaccaagacc
     1621 aggggcgggc c
//
LOCUS       DATBFZ010000465         4825 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_3278991, whole genome shotgun sequence.
ACCESSION   DATBFZ010000465 DATBFZ010000000
VERSION     DATBFZ010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 4825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4825
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3278991"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            321..1817
                     /locus_tag="VH419_14190"
     CDS             321..1817
                     /locus_tag="VH419_14190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044815.1"
                     /translation="MTDVGNVIESGANGRLLTAREDLSALLTAATGEPKVVLHFHGGL
                     VDREAGLAISARLGAVYRDAGAFPITFVWHSGLMEVLSGNLRQIAQEQLFRKLRTKVL
                     KYAVGRLEEAAGLKAGLGPAPPPPESQVEEELRLALEEEEEEREPYADLTGEPDEAAD
                     AAAAQSLERELRGDPVVQGVVAAVVLDEGDVADVDGGTRGLGVTPVAPGSLDGDIRTA
                     IVDDNQDAGERGLLSTAALVKKAVEVFLKVLQRFRAHTDHGVYVTVVEELMRALYAAS
                     VGAAVWSAMKGETEDTFKLDAQERAGHAFVEDFARMLADGVRPELTLVGHSTGAVFIV
                     NLLEALDQKRADPEHPLPADFTVKHVVFLAPALRSDLFAGTLQRHASLVEDFRMFSMS
                     DEAERADRLVGPVYPRSLLYLVSGLLETDQDGKSAVVPIVGLQRWCDGVRATTPELQA
                     VKSYIEEPGHVVWSPAGGSLGFSSAATTHSGFDDDDEVLASVKHLIQN"
     gene            1820..2893
                     /locus_tag="VH419_14195"
     CDS             1820..2893
                     /locus_tag="VH419_14195"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HEY7044816.1"
                     /translation="MVANPNHFALVVGINRYPGLSDLRFAKNDAADFAGWLVDPVGGG
                     CDPEHVKLIHVTEADEAAFGDWSEALPDWGRVTKALGKLHEEARAARSQDPSFWKDSR
                     LYVFASGHGIAPMDASGAYLTAEATLDQLGYNVDAATYANYYRQGSLFRQVIFLMDCC
                     RQRIPGAAALDPPFTRNDDPAPDRVDIVIGYATAYGDVAREPVGPVEDPDAQRGFFTR
                     ALLDGLRNGVGAADGSVDVASLERHMRNHVESATNGSSRRQSTRFVVESDEPVILRAP
                     DPTRAAVPPVAHKVVLRFQQDPPAQVKLLDGSLEQLSAVDVVEGAAVVELSDGIYGVT
                     VAGPPNVFPFSVAGSEVTVDVVL"
     gene            2880..4220
                     /locus_tag="VH419_14200"
     CDS             2880..4220
                     /locus_tag="VH419_14200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044817.1"
                     /translation="MSSSDTARGAGQLRVLAESAAEIEVVDSSAAVVAQGSGIVEVSL
                     PSGIYQVRASTSAGLQTPLAVVAQDETTEVLIRPGEPIAPVGLSVSEDQLPTSTMVDE
                     IATGAASGEGGVLYVMVRSVDASAAVVPDGVSVWRPGSARAVLQRSDWRSTATAAATA
                     VAVPAGWSVVESTGAPALARSVYVWPGRTTGLFALSVKGTVNWFDATVHSWPTGEPWS
                     RQPGMLDAELAMAALRTGRLVADDASASGDPYQVLLGGLTAAMTGRRTAPPSIDRSLR
                     DQCAETADWWLLFDHGFDRDHDHGDVKWRPLSTAPVFSLCIDLALERDRRRGDAFAPR
                     SVVGTIATHALRGSIWACWPPSIDADPPPPSPPMDADVTFGPPGPSRREALPRQLEKY
                     LSTVAEIEFGGSLQETAASLDAKDVSAAAHVPLSVAEAGLEWARRSERPSPEWL"
     gene            complement(4291..4364)
                     /locus_tag="VH419_14205"
     tRNA            complement(4291..4364)
                     /locus_tag="VH419_14205"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4328..4330),aa:Asp,seq:gtc)
     gene            complement(4416..4488)
                     /locus_tag="VH419_14210"
     tRNA            complement(4416..4488)
                     /locus_tag="VH419_14210"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4452..4454),aa:Glu,seq:ttc)
     gene            4641..>4825
                     /locus_tag="VH419_14215"
     CDS             4641..>4825
                     /locus_tag="VH419_14215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEY7044818.1"
                     /translation="MAQERVQVRRSAERFTTVGEGVRTRHLLSFADHYDPDLTSLGPL
                     VAHNDEVVEPGSGYAPHR"
BASE COUNT          768 a         1583 c         1699 g          775 t
ORIGIN      
        1 cgtacgcgag gctcacgtcg aaactctggt catgaccacg ccagcgcagc acgacctcga
       61 gttcggcgct gtcgcgtgcc acgtccaccc cccgatgcgc tcggcctcag catgtgcatg
      121 aggccacctt cgaacgtact cccggcgagc aagcatcatg ggcgcagcgc ggcatcgtgc
      181 cgatgacgcc tcccggcgtc gcaccaagcg ctcaccggcg accttgacgg tcctgggagc
      241 cgagagcacg cttgcctaac agcacgacac gtggacgatt cggtcgggga ccgtcggtcg
      301 cgcgtctcca gggggcaggc atgaccgacg tcgggaacgt catcgagtcc ggtgccaacg
      361 gacgcctcct caccgcgcgc gaggacctct ccgccttgtt gacggctgcg accggagagc
      421 cgaaagtcgt cctgcatttc catggcggac tggtggaccg agaggccggg ttggccatct
      481 ccgctcggct gggtgctgtc tatcgcgatg ccggggcctt cccgatcacc ttcgtctggc
      541 acagcggcct gatggaggtg ctctcgggca acctgcggca gatcgcgcag gagcagctgt
      601 tccggaagct ccggaccaag gtcttgaagt atgccgtcgg acggctcgag gaggcagccg
      661 gtctcaaggc cgggctgggc ccggcgccac cgccgccaga aagtcaggtc gaggaagaac
      721 tccggctggc actggaggag gaggaggagg agcgcgagcc gtacgccgac ctgaccggtg
      781 agccggacga ggcggcggac gccgcggcgg cgcagagtct ggagcgcgag ctccgcgggg
      841 accccgtcgt ccaaggtgtc gttgcggcgg tggtgctcga cgagggcgac gttgcggacg
      901 tggatggcgg gaccagaggc ctgggggtga ccccggtggc tcccggatcg ctcgacggtg
      961 acatcaggac ggcgatcgtc gacgacaacc aggacgccgg cgaacgcggg ctgctgagca
     1021 ccgccgcctt ggtgaagaag gctgtggagg tgttcctcaa ggtgctgcaa cggttccgtg
     1081 cgcacaccga ccacggcgtg tacgtcacgg tcgtcgaaga gctgatgcgg gcgctgtacg
     1141 ccgcctcggt cggcgccgcg gtgtggtcgg cgatgaaagg cgagaccgag gacaccttca
     1201 aacttgacgc gcaggagcga gcagggcacg cgttcgtcga agactttgcc cggatgctcg
     1261 ccgacggcgt gcggccggag ctcacgctgg tcgggcacag caccggcgcg gtgttcatcg
     1321 tgaacctgct cgaggccctg gaccagaagc gcgcggaccc cgaacacccg ttgccggctg
     1381 acttcaccgt gaagcacgtc gtgttcctgg cgccggcgct tcgctcagac ctgttcgccg
     1441 gcacgttgca gcggcacgcc tcgcttgtcg aggacttccg aatgttctcc atgtccgacg
     1501 aggccgagcg cgcggaccgg ctcgtcggcc cggtgtaccc acgttccctg ttgtacctgg
     1561 tgtcaggact cctcgagacc gaccaggacg gaaagtcggc cgtggttccc atcgtggggc
     1621 tgcagcggtg gtgtgacggc gtacgcgcga cgacgccgga gctgcaggcg gtcaagagct
     1681 acatcgagga gcctgggcac gtcgtgtggt cgccggccgg cgggtcgctg gggttctcgt
     1741 ctgcggcgac gacccactcg gggttcgacg atgacgacga ggtgctggcg agcgtgaagc
     1801 acctgatcca gaactgatca tggtcgccaa cccgaaccac ttcgcgctcg tcgtcggcat
     1861 caaccgctac cccggtctct cagacctcag attcgccaag aacgacgctg ctgacttcgc
     1921 cggctggttg gtcgaccccg tcggcggggg ttgcgacccg gagcatgtga agctcatcca
     1981 tgtcacggaa gccgacgagg cggcgttcgg cgactggtcg gaggcgctgc ccgactgggg
     2041 acgggtcacc aaggcgcttg gcaagctgca tgaggaggcg cgtgctgctc gaagccagga
     2101 tccctcattc tggaaggaca gcaggctgta cgtcttcgct tctgggcatg ggatcgcgcc
     2161 gatggacgcc agtggtgcct acctcaccgc tgaggcgaca ctggaccagc tcggctacaa
     2221 cgtcgatgcc gcgacgtacg cgaactacta ccggcagggc tcgttgttcc gacaagtcat
     2281 cttcttgatg gactgctgcc gtcaacgcat ccccggtgca gcggcgttgg accctccgtt
     2341 cactcgcaac gacgaccccg cgcccgacag ggtcgacatc gtcatcgggt acgcgacggc
     2401 ctacggcgat gtcgcccgcg agccggtagg cccggtcgag gatcccgatg cacagcgcgg
     2461 cttcttcacc cgggcactgc tggacgggct ccggaacggt gtcggcgccg ccgacggttc
     2521 ggtggacgtt gcgagcctgg agcggcacat gcgaaaccac gtggagagcg cgacgaacgg
     2581 ctcgagtcgc cgacagagca ccaggttcgt ggtcgagtcc gacgagccgg tcatcctgcg
     2641 cgccccggat cccacccgag ccgccgtacc accagtcgct cacaaggtcg tgctgcggtt
     2701 ccagcaggat cccccggctc aggtgaagct cctcgacgga tcgctcgagc agctcagcgc
     2761 agtggatgtc gtcgaaggag ccgccgtggt cgagttgagc gacggcatct atggggtcac
     2821 cgttgcgggc cccccgaacg tgttcccgtt ctcggtcgcc ggcagtgagg tgacggtgga
     2881 tgtcgtcctc tgacacggcc cggggggcag ggcagctccg ggtcctggcg gagtcagctg
     2941 cggagatcga ggtcgtcgac tccagcgccg cggtggtcgc tcagggctcc ggcatcgttg
     3001 aggtgtcgct gcccagcggc atctaccagg tgagggcttc gacgagcgcc ggtctgcaaa
     3061 cgcccctggc ggtcgttgcg caggacgaga cgaccgaggt cctcattcga cccggcgagc
     3121 cgatcgctcc ggtgggactg tcggtgtcgg aagaccagtt gccgacctcg accatggtcg
     3181 acgagatcgc caccggagcc gcgtcgggtg agggtggcgt cctctatgtc atggtgcggt
     3241 cagtcgacgc gagtgccgcc gtcgtacccg acggagtgag tgtctggcga cctggcagtg
     3301 cccgggctgt gttgcagcgt tccgactggc gcagcacagc caccgccgcc gcgacggcgg
     3361 tcgccgtgcc cgccggctgg tctgtcgtcg agagcaccgg ggcacctgcg ttggcgcgat
     3421 ccgtctacgt ctggcctggc cgcacaaccg gcctgttcgc gctttcggtc aaggggacgg
     3481 tcaactggtt cgacgccacc gtgcacagct ggccgaccgg cgagccttgg tcacggcagc
     3541 caggcatgct cgacgcagaa ctggccatgg ccgccctgcg caccggccgg ctggtggcgg
     3601 acgacgcgtc cgcgtctggt gatccttacc aggtgctgct cggtggcttg accgcagcca
     3661 tgaccggacg gcgaaccgcg cccccatcga tagaccggtc actgcgcgac cagtgcgcgg
     3721 agactgcgga ctggtggctg ttgttcgacc acggtttcga ccgagatcac gaccacggtg
     3781 acgtgaagtg gcggccactg tccacggctc cggtgttcag cctctgcatc gacttggccc
     3841 tggagcgtga tcgtcggcgc ggtgacgcgt tcgccccgcg gtccgtcgta gggacgatcg
     3901 cgacccatgc gctgcgcggc tcgatctggg cgtgctggcc accgtccatc gacgccgatc
     3961 cgccgccgcc gtcgccgccg atggatgcgg acgtcacatt cggaccgcct ggtccgagca
     4021 ggcgggaggc attgccgcgg cagctggaga agtacctgtc cacggttgcc gagatcgagt
     4081 tcggcggttc gctccaagag accgccgcgt cactggacgc caaggacgtc agtgccgcgg
     4141 cgcacgtacc gctgtcggtc gcggaggccg ggctcgagtg ggccaggcgg agtgagcgcc
     4201 cttcgcccga gtggttgtga gccgacatgt actcatgaag aaacccccgg tgatctcggt
     4261 cgtctcgatc aacgggggtt gacgttgcag cgaccccgac gggactcgaa cccgcggcct
     4321 tcgccgtgac agggcgacgc gctaaccaac tgcgctacgg ggccagtgtg cacatctcga
     4381 tgtgcggttc gcgcgccaaa ctttagcgca cgaagcaccc ccaacgggat tcgaacccgt
     4441 gctaccgccg tgaaagggcg gcgtcctagg ccactagacg atgggggcct aagcccaggg
     4501 ctttgtcagc aactcgcctg caaataccgt cggggaccga ccaagcatag ggggtcggct
     4561 ggccgccttc caaaccgata cggacgcctg cgccggccgc gcagagggcc cggcagactg
     4621 gcgacgagga ggctcgggca gtggcgcaag agcgggtgca ggtgaggcgg tcggccgagc
     4681 ggttcacgac cgtcggcgag ggtgtccgga cccgtcatct gctgtcgttc gcagaccact
     4741 acgatcccga cctgacgtcg ctcggcccgc tggtcgcgca caacgacgag gtcgtcgagc
     4801 cggggtccgg ctatgcgccc catcg
//
LOCUS       DATBFZ010000466         8996 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_1967270, whole genome shotgun sequence.
ACCESSION   DATBFZ010000466 DATBFZ010000000
VERSION     DATBFZ010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 8996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8996
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1967270"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="VH419_14220"
     CDS             complement(<1..523)
                     /locus_tag="VH419_14220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002544240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY7044819.1"
                     /translation="MRLDHLSFAVGADGLDGTTERLSTLLGEEFVDGGVHPRFGTRNR
                     VLPLTHGQFLEVVEVLDHPASDKAPFGQAVRARSEAGGGWLGWVVAVDDLAPVEKRLE
                     REAVPGNRHRPDGIELRWKQIGVKGLQSDPQLPFFVQWESPAAEHPSVGASGEVGLER
                     VEIAGDPRRVSSWL"
     gene            complement(641..1402)
                     /locus_tag="VH419_14225"
     CDS             complement(641..1402)
                     /locus_tag="VH419_14225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008354840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044820.1"
                     /translation="MPEVAPDFPRAFVEFDDPSDETQRFKVDLTWLTSRWTCIYGAGC
                     KGIYADRPDDGCCTLGAHFSDKEDEKRTRRFVKLLTAEDWQFHKVGHGKNAWIETDED
                     GERKTKVHEGGCIFLNRPGFSGGAGCALHAWALRNGHHPLETKPNVCWQLPIRRLFRE
                     VERGDKTSYTEVTITEYDRRGWGAGGHDLDWYCSGNTEAHIGTEPVYLANRPELVELM
                     GQRAYDVLAEHCRDFERRRSTLTLHPASKRRSKRR"
     gene            complement(1413..2270)
                     /locus_tag="VH419_14230"
     CDS             complement(1413..2270)
                     /locus_tag="VH419_14230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020578105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY7044821.1"
                     /translation="MTERLVRLPGTGAVDLCLEEFGDPEDPTVLLIAGAAASMDLWDP
                     ELCRRLAETGRHVVRYDHRDTGRSTTSPPGRPDYRSEDLFRDPVRLLDALEVGDAHLV
                     GMSMGGGVAQVVAIQHPDRVCTLTLMSTSAAGERSDPTRLPPAAPQLVTTFENPRPEP
                     DWNDREAVIAHLVETERPYAGSLGFDEEASRRRNARVVDRSIDVASAANHWSLEGDSP
                     TFAMSAIGVPTLVLHGSADPLFPLPHGESLAAEIPDARLVVLDGVGHEVPPPLLWDTV
                     VDEIARHTA"
     gene            2415..3338
                     /locus_tag="VH419_14235"
     CDS             2415..3338
                     /locus_tag="VH419_14235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007078928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HEY7044822.1"
                     /translation="MRLGVLDVGSNTAHLLVVDAYRGAPPLPASSTKQALRLAEHLTP
                     DGALSDKGVRSLVSFIEVALVEAEDRGCESVMAFATSAIREATNTDEVLATVHAKTDV
                     ELAVLSGDDEAKLTFLAVRRWFGWSSGRLAVFDIGGGSLEIAAGDDERPDVYHSLELG
                     AGRLTRGWLPDDVPTTEQIRALRRHIRAQIARDAGPLLRAGVGDHTVATSKTFRSLAR
                     VCGAAASEEGPFVRRSLARVDLEKKLPKIAAMTDTERANLPGVSAARAHQLVAGGLVA
                     DAVMDLFGIDALEICPWALREGVILEKLDLL"
     gene            3510..4316
                     /locus_tag="VH419_14240"
     CDS             3510..4316
                     /locus_tag="VH419_14240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012923956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEY7044823.1"
                     /translation="MSSASVYPESTPSAFELASRLGYDAVEVMVGIDPVSQDVDAIKH
                     LVDHHQIPVCSVHAPCLLITQRVWGSEPWAKLERSAAMASELGAATVVVHPPFRWQRD
                     YARGFVEGIARLEADTGVDFAVENMYPWRASKRELQAYLPGWNPVDHDYAHVTLDLSH
                     TSIAGTDPVRMVHELGPRLRHVHLADGTGSARDEHLVPGEGDQPCAEVLELLAERAYD
                     GNLVIEISTRKAASRADRLADLAEALAFTRLHFVAREPGYLTVLGDTSAD"
     gene            complement(4457..4678)
                     /locus_tag="VH419_14245"
     CDS             complement(4457..4678)
                     /locus_tag="VH419_14245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011753681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding carrier protein"
                     /protein_id="HEY7044824.1"
                     /translation="MSHHVNAELVANVLKVVAAPGDTVTPGDTLVILESMKMEIPVTT
                     EVSGTILEIGVSEGDVVTEGDLIADIDDA"
     gene            complement(4802..4978)
                     /locus_tag="VH419_14250"
     CDS             complement(4802..4978)
                     /locus_tag="VH419_14250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044825.1"
                     /translation="MTATASSAESPATKRETMPLDIGIELTKSLMLSLRDIISSERRN
                     NAILLGDCRRRIRS"
     gene            4989..5774
                     /locus_tag="VH419_14255"
     CDS             4989..5774
                     /locus_tag="VH419_14255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009075674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HEY7044826.1"
                     /translation="MADGLTATLDYLGEDTLDLTQAQHTVDAYVEVLKHLADAGLTRG
                     AEVSVKLSAVGQALPGDGERIALDNARAICQAASNAGTTVTLDMEDHTTTDSTLSILR
                     ELRQDFPSTGAVLQAYLHRTESDCRDLAYEGSRVRLCKGAYKEPESVAFQDKHQVNLS
                     YVRCLKVLMAGSGFPMIASHDPRLIEIAGALASRYQRSQGSYEYQMLYGIRPEEQKRL
                     ASRGERVRVYVPYGDEWYGYLMRRLAEKPANLAFFARSLISKS"
     gene            5817..6623
                     /gene="proC"
                     /locus_tag="VH419_14260"
     CDS             5817..6623
                     /gene="proC"
                     /locus_tag="VH419_14260"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020579491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEY7044827.1"
                     /translation="MTVAILGAGVMGETVVSGLVRAGRKPADLLMSERRAARAAELQE
                     RYGVDVVSNVDAARKADTLMLVVKPQDMPDLLDEIAPVVKPGQLVVSLAAGITTGYIE
                     GRLTDGVPVVRVMPNTPALVDEGMAAISRGSHCDEAHLVEAEELLRATGKVIRVPEKQ
                     QDAVTAISGSGPAYLFFVVEAMIEAGVHLGLPRSTATELVIQTMVGSAKLLRETGEHP
                     TVLRERVTSPGGTTAAAVRELEDHKVRAAFMTALESARDRSRALARGDDE"
     gene            6729..7904
                     /locus_tag="VH419_14265"
     CDS             6729..7904
                     /locus_tag="VH419_14265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020388009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin utilization protein AcuC"
                     /protein_id="HEY7044828.1"
                     /translation="MSGVGCLVFDEALTAYDFGHNHPMAPLRVDLTIRLARELGVLDG
                     LDVVPAPSATDDELELIHTEAYIEAVRKISDNPDQDDLLRGLGTDDNPTFAQMHQASA
                     HIVGATLEAARRVWDGRALHAANISGGLHHAMADCASGFCIYNDPAIAIAWLLRAGAE
                     RVAYVDIDVHHGDGVQAAFYDNPRVLTISLHESPRTLFPGTGDPSEIGGAGAEGMSVN
                     VAVPPGTGDDGWLRAFHAVVPPLLHAFEPTILVSQHGCDSHMDDPLAHLMLSVDGQRA
                     AHQAIHELAHEVCAGRWVVTGGGGYAVVDVVPRTWSHLLAVVAGSPVEPQTSTPRGWQ
                     DEVVARTGRPAPAWMTDGRTPEYADWSAGYDPASWLDRSVELTRRAAFPFHGIPLDY"
     gene            8175..8384
                     /locus_tag="VH419_14270"
     CDS             8175..8384
                     /locus_tag="VH419_14270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012923942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY7044829.1"
                     /translation="MTSHTTGEGISEVKFLTVAEVAAAMRVSKMTVYRLVHAGDLPAV
                     RVGRSFRVPEDAVNEYLRKSYFQAG"
BASE COUNT         1370 a         3055 c         3136 g         1435 t
ORIGIN      
        1 cgagccagga agagacccga cgcggatcgc cggcgatctc gaccctctcc agcccgacct
       61 cgcccgatgc gccgaccgac gggtgctccg cggcagggct ctcccactgc acgaagaacg
      121 gcagctgcgg atcagactgc agccccttga cgccgatctg cttccaccgc agctcgatgc
      181 cgtcggggcg atgccggttg ccaggcacgg cctcgcgctc gagtcgcttc tccactggcg
      241 cgaggtcgtc gaccgccacc acccagccga gccacccacc gccggcctcg ctgcgcgccc
      301 gcacggcctg cccgaacggc gccttgtcgg acgccgggtg gtcgaggacc tcgacgacct
      361 cgaggaactg cccgtgggtg agcggcagca cccggttgcg ggtcccgaaa cggggatgaa
      421 cgcctccgtc cacgaactcc tcgccgagca gcgtggacag ccgctccgtc gttccgtcca
      481 ggccatcggc cccgacagca aacgagagat ggtccaggcg catgccgttc attttgaccg
      541 cacgagttcc ggcgccggtc accggggtcg agttgggtga cgcctgccga ggagtctgcg
      601 ggaggcgctg ccggcctggg tgagcgtcgg ccgggcgtgc tcagcgacgc ttcgatcgcc
      661 gtttcgacgc cgggtgaagg gtgagcgtcg agcgtcggcg ttcgaagtcg cggcagtgct
      721 cggccaagac gtcgtacgcg cgttggccca tcagctcgac gagctccggc cggttggcca
      781 ggtagacggg ctcggttccg atgtgcgcct cggtgttgcc ggagcagtac cagtccaggt
      841 cgtgaccgcc ggctccccag ccgcgccggt cgtactccgt gatcgtcact tcggtgtacg
      901 acgtcttgtc gccccgctcg acctcacgga acagccggcg gatcggcagc tgccagcaga
      961 cgttcggctt ggtctccagt gggtggtggc cgtttcgcag ggcccacgca tgcagggcac
     1021 agccggcgcc tccggagaag ccgggacggt tgaggaagat gcacccgccc tcgtggacct
     1081 tcgtcttgcg ttcaccgtcc tcgtcggtct cgatccacgc gttctttccg tggccgacct
     1141 tgtggaactg ccagtcctcg gcggtcagca gcttgacgaa tcgacgggtg cgcttctcgt
     1201 cctccttgtc ggagaagtgc gcgcccagcg tgcagcaccc gtcgtcaggc cggtcggcgt
     1261 agatgccctt gcagccggcg ccgtagatgc aggtccaccg cgaggtcagc caggtcaggt
     1321 cgaccttgaa ccgttgcgtc tcgtcgctcg ggtcgtcgaa ctcgacgaag gcgcggggga
     1381 agtcgggagc tacctcgggc acgttctcca gcctaggcgg tgtgccgggc gatctcgtcg
     1441 acgaccgtgt cccacagcaa cggtggaggt acctcgtgac cgactccgtc gaggacgacc
     1501 agccgggcgt cagggatctc ggcggccagc gactcaccgt ggggcaacgg gaagagcgga
     1561 tcggcgcttc cgtgcagcac gagcgtgggt acgccgatcg cgctcatcgc gaaggtcggg
     1621 gagtcgcctt cgagactcca atggttcgcc gccgacgcga cgtcgatcga gcggtcgacg
     1681 acccgagcgt tgcgacgacg gctggcctcc tcgtcgaagc cgagcgatcc ggcgtacggg
     1741 cgttcggtct cgacgaggtg cgcgatcacc gcctcgcggt cgttccagtc cggctcggga
     1801 cgcgggttct cgaacgtcgt gacgagctgt ggcgctgctg gcggaagcct ggtcgggtcg
     1861 ctgcgctctc cggccgcgct cgtcgacatc agcgtcagcg tgcacacccg gtcgggatgc
     1921 tggatggcaa cgacctgcgc gacacctcca cccatcgaca taccgaccag gtgggcgtcc
     1981 ccgacctcca gcgcgtcgag cagccgtacc gggtcgcgga acaggtcctc gctgcggtag
     2041 tccggccggc cgggcggcga ggtggtggac cgccccgtgt cgcggtgatc gtagcgaacg
     2101 acgtgacgtc ccgtctcggc cagtcggcgg cacagctccg ggtcccagag gtccatcgat
     2161 gcagccgcac cggcgatgag caggacggtg ggatcctccg ggtcaccgaa ctcctccagg
     2221 cacaggtcaa ccgccccggt tcccggcagg cgcaccagtc gctcggtcat ggcagcagta
     2281 caccgtgagt ccatggcggg cgcacggcga gtgctccgcg agggcgcgta catcggcccg
     2341 tcacgaccga cccgcgctgc cacggcaggt ccggacagcg gcccgcgagc cagcaggtgt
     2401 cctaacctcg acccatgagg cttggtgttc tggacgtcgg ctcgaacacc gcgcacctgc
     2461 tcgtcgtcga cgcctatcgg ggcgcgcctc cactgcccgc atcgtcgacc aagcaggcgc
     2521 ttcggctggc cgagcacctc acgccggacg gcgcgctgtc ggacaagggc gtccggtcgc
     2581 tcgtgtcgtt catcgaggtc gcgctcgtcg aggcggagga ccgcggatgc gagtcggtga
     2641 tggcgttcgc cacgtccgcg atccgcgagg ccaccaacac cgacgaggtg ctggcgacgg
     2701 tgcacgccaa gacggatgtg gagctcgccg tactgagcgg ggacgacgag gccaaactga
     2761 ccttcctggc ggtccgacgg tggttcggct ggtcgtcagg acgacttgcc gtcttcgaca
     2821 tcgggggtgg ctcgctggag atcgcggcgg gtgacgacga acggccggat gtctaccact
     2881 cattggagct gggggccggt cggctcactc gcggctggct cccggacgac gtcccgacga
     2941 cggaacagat ccgcgcgctg cgtagacaca ttcgcgcgca gatcgcccgg gacgccgggc
     3001 cgttgctgcg cgccggcgtg ggcgaccaca cggttgccac gtccaagacg ttcaggtcgc
     3061 tcgcgcgggt ttgcggtgcg gcggcgagcg aggagggtcc gttcgtacgc cggagtctcg
     3121 cgcgcgtcga cctcgagaag aagctgccca agatcgcggc catgacggac accgagcgcg
     3181 ccaatctgcc cggtgtctcg gcggcgcggg cgcaccagct ggtggccggc ggactggtcg
     3241 cggacgcggt gatggacctc ttcggtatcg acgccctgga gatctgtccc tgggcgctgc
     3301 gcgaaggggt catcctggaa aagctcgacc tgctctgacg caggctccgc cgaacagtca
     3361 cgatctgcgt acagtcgtac ccccgcatgg cgccacggcg actggagtgc cgaccgcctg
     3421 cctacagtgt tggagtgagc gacgacgacg ccgatcctcg agacctggag ccggtgagcc
     3481 cggtccgggt cccgaccgcg actgtgggga tgtcgtcggc gtcggtctat cccgagtcca
     3541 ccccgagcgc cttcgagctg gcctcgcggc tcggctacga cgccgtggag gtgatggtcg
     3601 ggatcgaccc ggtgagtcag gacgtcgatg ccatcaagca cctcgtcgac caccaccaga
     3661 tcccggtctg ctcggtgcac gccccgtgcc tgctcatcac gcaacgggtc tgggggagcg
     3721 aaccctgggc caagctcgag cggagtgcgg ccatggcgag cgagctcggc gcagcgaccg
     3781 tcgtcgtcca cccgccgttt cgctggcagc gcgactacgc acgcggcttc gtggagggga
     3841 tcgcccggct cgaggcggac actggcgtcg acttcgcggt ggagaacatg tacccctggc
     3901 gggcgtccaa gcgggagctg caggcctacc tgcccggctg gaacccggtc gaccatgact
     3961 acgcccacgt cacgctcgac ctgtcgcaca cgtcgatcgc cggaaccgat ccggtccgga
     4021 tggtccacga gctcgggccg cggctgcggc acgtccacct ggcggacggc accggctcgg
     4081 cccgtgatga gcacctggtg cccggtgagg gcgaccagcc gtgcgcggag gtgctggagc
     4141 tgctggccga gcgtgcgtac gacggcaacc tcgtcatcga gatcagtacg cgcaaggcgg
     4201 cctcccgcgc cgaccggctg gccgacctgg cggaggcgct cgccttcacc cggctgcact
     4261 tcgtggcgcg cgaacccggc tacctcactg tcctgggcga cacctcggcc gactgaggct
     4321 gtccgctccg cgagtggacc tcgccgagct gtccgcgatc gaccagctgg tcgtcggagg
     4381 cttgaatcgt cgctgcggtt cgctcggcgc cgccggcctt tgacggcgta ccgcttgggc
     4441 tctgcgacga tgcgggtcag gcgtcgtcga tgtcggcgat gaggtcgccc tcggtgacga
     4501 cgtcgccctc ggagacgccg atctccagga tcgtgccgga cacctcggtg gtcaccggga
     4561 tctccatctt catcgactcg aggatcacga gtgtgtcgcc cggcgtcacc gtgtctccag
     4621 gcgcagcgac caccttcaag acgttcgcca cgagctcggc gttcacgtgg tggctcatgc
     4681 tgctccttgc ctcggacggc cacggcacag gatagacccg ggcgcggcac cggcaggcgg
     4741 acgtggagca tggcggcagc atgatggtgg cggggccaca cggcgtaccc tgtcctcgcg
     4801 gtcacgaccg gattcgccgc ctgcagtcgc caaggaggat cgcgttgttg cgacgttcgc
     4861 tgctgatgat gtcgcgcagt gacaacatca gggatttcgt cagctcgatg ccgatatcga
     4921 gcggcatcgt ctcgcgcttc gtcgccggcg actccgccga cgatgcggtc gcggtcacgg
     4981 agcacctcgt ggccgacggc ctcaccgcca cgctcgacta tctcggtgag gacaccctcg
     5041 acctgaccca agcgcagcac acggtcgatg cgtacgtcga ggtcctcaag cacctcgccg
     5101 acgccgggct cacccgcggc gcagaggtct cggtgaagct gtcggcggtc ggacaggcac
     5161 tgccgggtga cggtgaacgg atcgcgctcg acaacgcgcg ggcgatctgc caggccgcga
     5221 gcaacgccgg tacgacggtc acgctcgaca tggaagacca caccacgacc gactcgaccc
     5281 tgtcgatcct gcgcgagctc cggcaggact tcccgagcac cggcgctgtc ctccaggcgt
     5341 acctgcaccg aacggagtcc gactgccggg acctggcgta cgaaggatcg cgggttcggc
     5401 tgtgcaaggg cgcctacaag gagccggagt cggttgcgtt ccaggacaag caccaggtga
     5461 acctgtccta cgtccggtgc ctcaaggtgc tgatggccgg gtcaggcttt ccgatgatcg
     5521 cctcgcacga tccgcggctc atcgagatcg ccggcgccct cgcgtcgcgc taccagcgca
     5581 gccaggggag ctacgagtac cagatgctct acggcatccg tccggaggag cagaagcggc
     5641 tggcgtcgcg cggcgagcgc gtccgcgtct acgttccgta cggggacgag tggtacggct
     5701 acctgatgag acgcctcgcc gagaagcccg ccaacctggc attcttcgcc cgatcactca
     5761 tcagcaagtc ctgacaccgc ccagccggcg gtcaagggca gacgggagag cacctgatga
     5821 ccgtcgccat tctcggtgcg ggcgtgatgg gggagaccgt cgtctccgga ctcgtccgcg
     5881 cgggccgcaa gcccgccgac ctgctgatgt ccgagcgccg tgctgcgcgc gcggccgagc
     5941 tgcaggagcg gtacggcgtc gacgtcgtct ccaacgtgga tgccgctcgc aaggccgaca
     6001 ccttgatgct cgtcgtcaag ccgcaggaca tgcccgacct gttggacgag atcgcgccgg
     6061 tggtgaagcc ggggcagctc gtcgtctcgc tcgccgcggg catcacgacc ggctacatcg
     6121 aggggaggct caccgacgga gtgccggtcg tgcgcgtcat gccgaacacg ccggcgctgg
     6181 tcgacgaagg catggcggcc atctcgcgag ggtcccactg cgacgaggca cacctcgtcg
     6241 aggcggagga gctgcttcgc gccaccggca aggtgatccg tgtcccggag aagcagcagg
     6301 acgcggtgac cgcgatctcg ggctcgggtc cggcgtacct cttcttcgtc gtcgaggcca
     6361 tgatcgaggc cggtgtgcac ctcgggctgc cgcgctcgac ggcgaccgag ctggtgatcc
     6421 agaccatggt cggatcggcc aagctgctcc gtgagacggg tgagcacccg acggtgctgc
     6481 gggaacgagt cacctcgccg ggcggtacga ccgccgcggc ggttcgcgag ctcgaggacc
     6541 acaaggtgcg ggcggcgttc atgactgccc tggagagcgc gcgtgaccgc tctcgtgcgc
     6601 tggctcgtgg cgacgacgag tagccgggtt ccgcggattg cgggaaacat catcggcacg
     6661 tcggcgtgtc gacctgagct ttcccgcatt tcgcggtcca ccagcccgcc tggcctagag
     6721 tcgagtgcat gtccggtgtc ggttgtctgg tcttcgacga ggcactgacg gcgtacgact
     6781 tcgggcacaa ccaccccatg gcgcccttgc gggtggacct gacgatccga cttgcgcggg
     6841 agctcggagt cctcgacggg ctcgacgtcg taccggcgcc gagcgccacc gatgacgagc
     6901 tcgagctgat ccacacggag gcctacatcg aggctgtgcg aaagatcagc gacaaccccg
     6961 accaggacga cctgctccga ggcctgggga ccgatgacaa cccgacgttc gcgcagatgc
     7021 accaggcctc cgcgcacatc gtcggcgcca cactggaggc ggcccgacgc gtctgggacg
     7081 ggcgggcact gcatgcggcg aacatcagcg ggggactcca ccacgcgatg gccgactgcg
     7141 ccagcggctt ctgcatctac aacgacccgg ccatcgcgat cgcctggctg cttcgcgccg
     7201 gcgcggaacg agtggcgtac gtcgacatcg acgtgcacca cggcgacggc gtacaggcgg
     7261 cgttctacga caacccgcgc gtcctcacga tcagcctgca cgagagcccg cgcacgctct
     7321 tcccagggac cggtgacccc agcgagatcg gtggggccgg cgcggagggc atgtccgtca
     7381 acgtcgctgt cccgcccggg acgggggacg acggctggct gcgcgccttc cacgcggtcg
     7441 tgccaccgct gttgcatgcg ttcgagccca cgatcctggt gagccagcac ggatgcgact
     7501 cgcacatgga cgacccgttg gcgcacctga tgctcagcgt ggacggacaa cgcgcggccc
     7561 accaggcgat ccatgagctg gcgcacgagg tgtgtgcagg ccgctgggtc gtgaccgggg
     7621 gcggcggtta cgccgtggtc gacgtcgtgc cgcggacatg gtcacacctg ctcgccgtgg
     7681 tggccggatc gccggtcgaa ccgcagacct ccaccccccg tggctggcag gacgaggtcg
     7741 tcgctcgcac aggacgcccc gcgccggcct ggatgaccga tggccggacg ccggagtacg
     7801 ccgactggag cgccggctac gaccctgcat cgtggctgga ccgcagcgtc gagctgaccc
     7861 ggagagcagc cttccccttc cacggcatcc cgctcgacta ctgaaccgcc cccggtgaaa
     7921 tggccggcgg gcatgccgat ccgatgggcc gatgtggagc gggaggctgt ggggtctgca
     7981 ccccatctgg gtgacttgat catcggcgct cggcgacacg ccgaccgggt gcgccatttc
     8041 cggcacaatt cggcggtcat ttttctccgt ctctttccca tcagcctcat ttgccactac
     8101 tctcacgatg agcacgggtg atggtgtctt cggtggggaa gccgaagagc gctcgcgccg
     8161 aaagttggtg cgtcatgacc tcacacacga ccggcgaagg aatttctgag gtcaagttcc
     8221 tgactgtggc ggaagtggcc gcggccatgc gggtgtcgaa gatgacggtc taccgactgg
     8281 tgcacgcagg tgacctgccg gccgtgcggg tcgggcggtc gttccgcgtc cccgaagacg
     8341 ctgtcaacga gtacctccgg aagtcctact tccaggctgg gtgagtcgcg ccccgcgcta
     8401 gcgccgggag cctgtgcgct ggtgccgtgg cggttcgccg cgggcaccag gtgaacccct
     8461 gcgtcgcgag gcagatctgg gactcctcat gttccgtaag cggagacgtt cgtgatggct
     8521 gcgctcgtcg cccggcttcc gtcgtgatga gctcgtgggt cggcaacgag gcgcggcagc
     8581 gttcgcgtct gttgagccgg tcggccgacg tcccgtcccg tctggctggt gggcggttgg
     8641 cggtttcgga cccacgccgg ggtcgccgag cggcggtttg catgcctacc caggcaaatc
     8701 cacccgtccc gtggcaggtt tgcctggcta cccgtccgta tggatggcta ccgaggcaaa
     8761 ccaccgatac atgtccgatt ggtggcgtag gtggctttcc ggaggcggcc cacaagaagc
     8821 aaggagtctg tctgcagggt tgttgcgggt agcttcagcg tcggccgaag tcgatgcagc
     8881 cgatccagtt gccgtcggcg tcgcggcggg cggcgatgcc cgagagaccg aacgagccgt
     8941 cgagcaggat gcggcggtgg tgcggcgagt gcaaccacat ccggatcatc cggtgt
//
LOCUS       DATBFZ010000467         6023 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_5414777, whole genome shotgun sequence.
ACCESSION   DATBFZ010000467 DATBFZ010000000
VERSION     DATBFZ010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 6023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6023
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5414777"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="VH419_14275"
     CDS             complement(<1..500)
                     /locus_tag="VH419_14275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044830.1"
                     /translation="MKRRTLDIIFSVGGLALAVLLAVLGIVLKSNADFAKNYVHDQLV
                     AQKITFTPADKLAPAEKKSDALVKYAGEPLATGKQAETYANDYIALHLSEVNNGKTYS
                     QTSNDARALRAQATAAQKAGAPNAAELDKQATALEAQVQTLFRGETLRGMLLTSYGFS
                     IFGDKA"
     gene            complement(630..1097)
                     /locus_tag="VH419_14280"
     CDS             complement(630..1097)
                     /locus_tag="VH419_14280"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEY7044831.1"
                     /translation="MAGLSLPFILRDHDSTHGPHVLDATECWERVRESGVGRLAFVVE
                     NWPVVLPVNFCTDGDDVVLRSGEGLKLSTLRRSPAVALEVDSFEPLYRSGWSVLLLGT
                     ASVVDDPDESGRLDALGLRPWAASERASWLRVHPLQITGRRLARAWRYPDLPI"
     gene            1314..2348
                     /locus_tag="VH419_14285"
     CDS             1314..2348
                     /locus_tag="VH419_14285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008361297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HEY7044832.1"
                     /translation="MKAAVVHQLGGPLTVEDRPAPRPGPGDVIVRLEASGLCHTDIHA
                     AHGDWPVKPTPPFVPGHEGVGRIEALGEGVSTRRIGERVAVPWLHVACGICEFCVAGW
                     ETLCLAQQNTGYSVDGCYAELVRAPASHVVPVPEGIDPLEAAPLTCAGVTTYKAVKVS
                     GARSSDLVAVFGVGGLGHLAVQYARIAGATVVAVDVTDDKLGLAKSLGATYTVNARTE
                     SVVDAIRDLGGADVAIATAADAGPLRAAFDSLRRRGRLVLVGLPARNELSLPIFETVL
                     NGISVIGSIVGTRGDLAEVFELHASGATRVVYETRALADVNAAFDDVLSGKVAARLVF
                     DLADEHLPSS"
     gene            2359..2844
                     /locus_tag="VH419_14290"
     CDS             2359..2844
                     /locus_tag="VH419_14290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HEY7044833.1"
                     /translation="MNEQVLGLSVWEQDLYDLLLDHVKSESEVLDRYGSLADAAPGHV
                     RFLVELIAEDEARHHALYEQWAEAIKAAGTFQHTEDGVPDLMPEKDPQPLIAAIDELL
                     ELEKRDKQQLKELDRRLKDVRETTIWPLLVELMAVDTRKHIRILEFLRKHARGTARSR
                     R"
     gene            3055..3522
                     /locus_tag="VH419_14295"
     CDS             3055..3522
                     /locus_tag="VH419_14295"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEY7044834.1"
                     /translation="MTTDLQTPMTILDRDECWNLMRSAPVGRLAVSVGAHPDIFPVNF
                     VIDHGTVVFRTAEGTKLAASLLGVAVAFEVDGYDPAAGEAWSVVVKGRAVEIQRMHEL
                     FDAMDLPLFPWHAAPKHRFVRIVPDEISGRRFHVVDRPAATTTESERHAAAPE"
     gene            3562..4026
                     /locus_tag="VH419_14300"
     CDS             3562..4026
                     /locus_tag="VH419_14300"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEY7044835.1"
                     /translation="MNVSAGLSDLAVLTHDDCMHRLVRGCVGRVGFVHDERPEVLPVN
                     YAADADGRVVFRAGPNSVLRGVVGQPVVFEVDGADASRRSGWSVCVHGSGLEITDADD
                     PLASQLRKLPVITWAPGRRDRWFAVVPAAVTGRELPISVTGDEGWFPGVPAS"
     gene            4162..4968
                     /locus_tag="VH419_14305"
     CDS             4162..4968
                     /locus_tag="VH419_14305"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY7044836.1"
                     /translation="MFESIVVPLDLEAGGDRALPIAGALARGAGVTLELITVSSPGMS
                     EEFDLYDLSQRARSTGAASTTRVLHGNNVADTILSFVDDRPNPLVVMGTRARGPLGEY
                     FLGSVSEAVLTSATDPVLLVGPRTDIDEPSPTPTLVIAVDGFAAATALAPAVTAWVES
                     FGGPPPWMVEVAPDEGDEDAGDSSPGRRLGDLLDGRGITVEWEVSQDRRPVDALVDFA
                     DHVADAVLAVASSRWTDPRPRFTSTARKLTQQAHHPVLVVPVRDAGDGAP"
     gene            4984..5640
                     /locus_tag="VH419_14310"
     CDS             4984..5640
                     /locus_tag="VH419_14310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY7044837.1"
                     /translation="MAESQRITVFLLDDHEIVRRGLRDLLESEGDIEVVGEASTQEQA
                     VGRVHALDPDVAVLDVRLEDGNGIEACREIRSLHPRTACLILTSFADDEALFQAIMAG
                     AAGYVLKQVRSADLVNAVRRVAAGQNLLDPVVTARVLERIRHGPEQDERVARLTEQER
                     HVLELLAEGLTNREIGERLHLAEKTIKNYVTALLSKMGMARRTEAAVYAARHLATREQ
                     "
     gene            complement(5665..>6023)
                     /locus_tag="VH419_14315"
     CDS             complement(5665..>6023)
                     /locus_tag="VH419_14315"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY7044838.1"
                     /translation="EEMAPSLGTHPVVTFEGPLDLALSAAVGDELVVALQELLSNVAR
                     HAHASRVTVSVVVAGGLVTLQVDDDGVGPPDGSQPSSGLGLHNLATRAERLDGHFALH
                     RGAESGTTAVWSARSA"
BASE COUNT          926 a         2064 c         2118 g          915 t
ORIGIN      
        1 atggctttgt caccgaagat gctgaacccg tacgaggtca ggagcatccc acgcagcgtc
       61 tcgccccgga acagggtctg gacctgggcc tcgagcgctg tggcctgctt gtcgagctcg
      121 gccgcgttgg gggcgccagc cttctgcgcc gcggtcgcct gcgcccgcag ggccctggcg
      181 tcgttgctcg tctgggagta cgtcttgccg ttgttgacct cgctcagatg gagggcgatg
      241 tagtcgttgg cgtacgtctc ggcttgcttg ccggtcgcca gcggctcgcc ggcgtacttc
      301 accagggcgt ccgacttctt ctcggctggg gccagcttgt cagccggcgt gaaggtgatc
      361 ttctgcgcga ccagctggtc gtgcacgtag ttcttcgcaa agtcggcatt cgacttcagc
      421 acgatcccga ggaccgccag cagcacggcg agagccaagc cacccacgct gaagatgatg
      481 tcgagagtcc tgcgcttcat ggtctctccg tttcctagtt cgatcgtccc gggcttcagt
      541 gctcggtact ggcgtccagt cttcgagcgt cggggtgccg gtcgggaggg accaaggtca
      601 tgacttccgc ggaccaacga cggctccggt cagatcggaa ggtccgggta gcgccaggcg
      661 cgcgccagcc ggcgacccgt gatctgcagc gggtggacgc gcagccatga ggcgcgctca
      721 ctcgccgccc atggccgaag tccgagcgcg tcgagcctcc ccgactcgtc cggatcgtcg
      781 accacagacg cggtgccgag cagcaggacg ctccagccgg agcggtacaa cggctcgaac
      841 gagtcgacct cgagcgcgac cgccggcgaa cgccgcagcg tcgagagttt gagaccctcg
      901 ccgctccgga gaacgacgtc gtcgccgtcg gtgcagaagt tcaccggcag gaccaccggc
      961 cagttctcga ccacgaaggc gagccgtcct actccgctct cgcgaacccg ctcccaacac
     1021 tccgttgcat cgaggacgtg cgggccgtgg gtggagtcgt ggtcgcggag gatgaacggc
     1081 agggacaagc cagccatgcg gtgacgatgc tccacccacg gctcgagccg aagggtccaa
     1141 ggtcccgact tcggcccgac ccgatcggag tccgacgcgt cccaccccgt taagggacga
     1201 acgtccctgt ctgcgtcgac cgagggccat ggtccttcgc tcggcgcagc ggttcgctgt
     1261 cttcgacttc ccgtcgcgac gcgtcgcggg cgggccagac gaaggagatc gcgatgaagg
     1321 cagctgtcgt ccaccaactc ggcggcccac tgaccgtgga agaccgcccg gcgcccaggc
     1381 cgggcccagg ggacgtgatc gtgcggctgg aggcgtcggg cctgtgccac accgacatcc
     1441 acgccgccca cggcgactgg ccggtcaagc cgaccccgcc gttcgtgccg ggacacgaag
     1501 gggtcggcag gatcgaggcg ctcggcgagg gagtctcgac ccggcgcatc ggtgagcggg
     1561 tcgccgtgcc gtggttgcac gtggcctgcg gcatctgcga gttctgcgtg gccggctggg
     1621 aaacgctgtg cctcgcccag cagaacaccg gctattcggt cgacggctgc tacgccgagc
     1681 tcgtacgggc gccggcgtcg cacgtggtcc ccgttcccga gggcatcgat ccgctcgagg
     1741 cggctccgct cacctgcgcc ggggtgacca cgtacaaggc ggtgaaggtg tcaggcgcgc
     1801 ggtcgtccga cctcgtcgcg gtcttcgggg tcggcggtct gggtcacctg gccgtgcagt
     1861 acgcccgcat cgcgggcgca accgtcgtcg ccgtggatgt cactgacgac aagctgggcc
     1921 tggccaagag cctcggcgcc acgtacacgg tgaacgcgag gacggagtcc gtcgtcgacg
     1981 ccatccgaga cctgggtggc gcggacgtcg ccatcgccac agccgccgac gccggaccgc
     2041 tccgcgccgc gttcgactcg ctccgccggc ggggccggct cgtgctcgtc ggtctaccgg
     2101 cccgcaacga gctctcgctg ccgatcttcg agaccgtgct caacggcatc tcggtcatcg
     2161 gctcgatcgt cggcactcgc ggcgatctcg ccgaggtgtt cgagctccac gcctcgggcg
     2221 ccacacgggt ggtgtacgag acgcgagcat tggccgacgt caacgctgcg ttcgacgacg
     2281 tgctgagcgg caaggtcgcg gctcggctcg tcttcgacct ggccgacgaa cacctgccat
     2341 cgagctgagg acgacatcat gaacgagcaa gtcttgggcc tgtcggtctg ggagcaggac
     2401 ctgtatgacc tgctcctcga ccacgtgaag tccgaatcgg aggtgctcga ccgatacggc
     2461 agcctcgccg acgccgcgcc cgggcacgtg cgcttcctcg tcgagctcat cgccgaggac
     2521 gaagcgcgcc accacgcgct gtacgagcag tgggcggagg cgatcaaggc ggccggcacg
     2581 ttccagcaca ccgaggacgg ggttcccgac ctgatgccgg agaaggatcc gcagccgctc
     2641 atcgccgcga tcgacgagct gctcgagctc gagaagcgcg acaagcaaca gttgaaggag
     2701 ctcgacaggc gactcaagga cgtccgcgag acgacgatct ggccgttgct cgtcgagctg
     2761 atggcggtcg acacgcgcaa gcacatccgg atcctcgagt tcctgcgcaa gcacgcccga
     2821 gggacggcgc ggtcacgacg gtgagttgag ggcggaccgg accggtcccg cgcttccggt
     2881 ccggtctccg aggcgaagtg cacctcgcgc gagcccgaaa gggcatcggt gaggtgcact
     2941 tcggccggtt cggggcggcg tttcaccgac gcggcgcctg tcgaccggcc tcgtcccacg
     3001 ggtcagggac cttcggccct aggtcgacaa gccagacagg gcgacgatca acccatgacg
     3061 accgacctgc agacaccgat gacgatcctc gatcgcgacg agtgctggaa cctgatgcgg
     3121 tccgcgccgg tcggccggct ggcggtctcg gtaggcgcgc acccggacat cttcccggtc
     3181 aacttcgtga tcgaccacgg cacggtggtg ttccgtacgg ccgagggcac caagctggcc
     3241 gcgtcgctgc tcggcgtcgc cgtcgccttc gaggtcgacg gctatgaccc tgcggccggc
     3301 gaggcgtgga gcgtggtggt gaagggccga gcggtcgaga tccaacggat gcacgagctc
     3361 ttcgacgcca tggatctgcc gctcttcccc tggcacgccg cgccgaagca ccgattcgtg
     3421 cgcatcgtgc ccgacgagat cagcgggcgc cggttccatg tggtggaccg tcctgccgcc
     3481 acgaccacag agtccgaacg acatgccgcc gctcccgagt gacggagcga gccgtcgagc
     3541 gatgcaccga ggaggtggct catgaacgtg agtgccggac tgagtgacct cgccgtgctc
     3601 acccatgacg actgcatgca ccggctggtg cgaggatgcg tcggccgggt cggtttcgtg
     3661 cacgacgagc gcccggaggt gctgccggtg aactacgccg ccgatgccga cgggcgcgtc
     3721 gtcttccgtg cgggcccgaa ctccgtcttg aggggggttg tgggccagcc ggtcgtgttc
     3781 gaggtcgacg gcgcggatgc gtcgcggcgg agcggctgga gcgtgtgcgt gcacgggagc
     3841 ggcttggaga tcacggatgc ggacgaccca cttgcgtcgc agctgcgcaa gcttcccgtg
     3901 atcacctggg cgccgggtcg gcgcgaccgg tggttcgccg tcgtgccggc ggctgtcacg
     3961 ggccgggagc ttccgatcag cgtgacgggc gacgagggct ggttccctgg cgtcccggca
     4021 tcgtgatcgg cgaacagacg gaccatgcca gctcggtggc ctccttgcgg ggcgacaccc
     4081 cgcctcgcgg ccgtcggacc gcgttgtggg acaatgactg ggctcattgc acgtggggtc
     4141 ctcggacagg aggctgagcc gatgttcgag tccatcgtcg tgcccctcga cctcgaggct
     4201 gggggcgacc gcgccctgcc gatcgccggc gcgctggcca gaggcgccgg cgtgacgctc
     4261 gagctgatca cggtgtcgtc gccagggatg tctgaggagt tcgacctgta cgacctgtca
     4321 cagcgggcgc gctcgaccgg cgccgcttcg acgacgcggg tgctgcacgg caacaacgtc
     4381 gccgacacca tcctttcctt cgtcgacgac cggcccaacc cgctcgtggt gatgggaacg
     4441 cgcgcccgtg gtccgctcgg cgagtacttc ctcggcagcg tgagcgaagc cgtgctcacg
     4501 agcgccaccg accccgtact gctcgtcggg cccaggacgg acatcgacga gccttccccg
     4561 acaccgacgt tggtgatcgc cgtcgatggg ttcgccgcgg ccaccgcgct ggcgcccgcg
     4621 gtgaccgcat gggtcgagtc gttcggaggc ccgccgcctt ggatggtcga ggtcgccccc
     4681 gacgaaggcg atgaggacgc cggcgactcg agccccggcc ggcggctcgg cgatctgttg
     4741 gacgggcgcg ggatcacggt cgaatgggag gtcagtcagg accggcggcc ggtggacgcc
     4801 ctggtcgact tcgccgacca cgtcgccgac gcggtgctgg ccgtggccag ctcgcgctgg
     4861 accgacccga ggccgcgctt cacgagcacc gctcggaagc tgacgcaaca ggcgcaccat
     4921 ccggtgctcg tcgtcccggt gcgcgatgcc ggcgacggcg caccctgagc gcgaggatca
     4981 ggcatggcag agagccagcg catcacggtc ttcctcctcg acgaccatga gatcgtccgc
     5041 agaggtctgc gcgacctcct cgagtccgag ggcgacatcg aggtggtcgg tgaggcatcg
     5101 acgcaggagc aggcggtcgg acgggtccac gccctcgacc ctgacgtggc cgtgctcgac
     5161 gtccgactcg aggacggcaa cggcatcgag gcgtgccggg agatccggtc gctccacccg
     5221 cgcacggcgt gtctgatcct gacgtcgttc gccgacgacg aggctctgtt ccaggcgatc
     5281 atggccgggg ccgccggcta cgtgctcaag caggtgcgct cagccgacct ggtgaacgcc
     5341 gtccgccgag tggccgccgg gcagaacctg ctcgacccgg tggtcacggc tcgcgtgctc
     5401 gagcgcatcc gccacggacc cgaacaggac gagcgggtcg ctcgcctcac cgagcaggag
     5461 cgccatgtgc tcgagctgct cgccgaggga ctcaccaacc gggagatcgg cgagcgcctt
     5521 cacctcgccg agaagacgat caagaactac gtcactgccc tgctgtcgaa gatgggcatg
     5581 gcgcggcgca ccgaggcagc ggtgtacgcc gcccgccacc tcgccaccag ggagcagtag
     5641 acgcgtcgcg tcgttcgaac gcggtcaagc cgaacgcgcc gaccacaccg cagtggtccc
     5701 actctccgcg ccgcgatgca gggcgaagtg gccgtcgagc cgctcggcgc gcgtcgcgag
     5761 gttgtggagc ccgagtcccg agctcggctg cgaaccatcc ggcggaccga cgccgtcgtc
     5821 gtcgacctgc aacgtcacga gcccgccggc cacgacgacc gagaccgtca cgcggctggc
     5881 gtgcgcgtgc cgggcgacgt tcgacagcag ctcctggagg gcgacgacga gctcatcgcc
     5941 gacagcggcc gacaatgcca agtcgagcgg accctcgaac gtgacgaccg gatgggtgcc
     6001 gagcgacggc gccatctcct cgg
//
LOCUS       DATBFZ010000468         7074 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_3606319, whole genome shotgun sequence.
ACCESSION   DATBFZ010000468 DATBFZ010000000
VERSION     DATBFZ010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 7074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7074
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3606319"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(151..900)
                     /locus_tag="VH419_14320"
     CDS             complement(151..900)
                     /locus_tag="VH419_14320"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020578521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY7044839.1"
                     /translation="MPLALVTGPTSGIGHGFAQALAAEGHDLMLVSRDRTRLDKVAAE
                     LAERHGVACDVIAADLADLDQTKVVEAWLADHPVDVLVNNAGFGKRKPFEANDIESEQ
                     ESFDVLVRAVMRLTHAALGPMLERGHGDIVNVSSVAGFVPRGTYGAHKAWVTSFSRWA
                     HLRYKRQGVRVVAVCPGFVRTEFHQRMDANISGIPGFMWLQADDVVGQALADLRAGKA
                     ISIPSFRWKALMAVSRLTPSGLVERVARRGR"
     gene            992..1792
                     /locus_tag="VH419_14325"
     CDS             992..1792
                     /locus_tag="VH419_14325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017934869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEY7044840.1"
                     /translation="MTTTLAIANQKGGVAKTTTVASLGTALVDQGLRVLLVDLDPQSC
                     LTFSLGIDPEELDASLHQVLMGQSKCAEVILETEDGPDLLPATIELATAELMLLTRPG
                     REHVLQAALEDVDADYDWVLLDCPPSLGVLTVSALSAADSVMIPLQCETLSHRGVGQL
                     LDTVHDVRRFTNPRLRVMGVLPTLYDSRPKHSRAVLETISETYDVDVIEPPIPRSIRF
                     AEAPAAGRSIFRTARSTRGAQAYRTLAENLANPTGAPRGSEAQSDEST"
     gene            1813..2520
                     /locus_tag="VH419_14330"
     CDS             1813..2520
                     /locus_tag="VH419_14330"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEY7044841.1"
                     /translation="MPSRREPLRRTRTPAGRMVAVGSAAAISLLTMAGALGFGPGAAM
                     AQTQRTPSSAATDSAPDHQSADRDAADPSVHATLDDGWLTYDDSMSKAPAAQPDETAL
                     PPRTGRGERIVYDISAQRVWLVSSDDSVERTYLVSGGKDESLLEPGRYHVYSMSPHAV
                     SFNHKETMNYMVRFATGQHAPIGFHDVPARLDGTLVESRSELGTPQSSGCIRQWIDDA
                     RALWEFAEIDTPVVVTA"
     gene            complement(2606..4123)
                     /locus_tag="VH419_14335"
     CDS             complement(2606..4123)
                     /locus_tag="VH419_14335"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012922930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY7044842.1"
                     /translation="MLPEIAEALAEDDITDAFPIQEMTLPIALMGTDLIGQARTGTGK
                     TLAFSIPILQRIVSPHDPDYDELAAPGKPQALVIAPTRELALQVTNDLSLAGKHRGTR
                     TVTVYGGVPYEPQLAALSAGVDVVVGTPGRLLDLEARKALDLAHVKVLVLDEADEMLD
                     LGFLPDVEKLIAKTPELRQTMLFSATMRGEVVTLARTHMRHPVNIRAESSDDRATVPA
                     TAQFVYQAHDLDKIEVIARIMQAENRGLSLIFTRTKRQAQRVAEELIERGFPAAPLHG
                     DMAQVARERAMKRFRDGKVEILVATDVAARGIDVVGITHVINHTCPEDENTYVHRIGR
                     TGRAGATGVAITFVDWADVTRWKVINKALDLPFDEPQETYSTSEHLYHDQGIPGDAKG
                     RIGKPREKPDAADLLENSRRGDRRSGGGRRDARSRGDGSRGDRSTDGASTGRSRSRSR
                     RRTRSGQAVAGTPETSPAQSATAASEQGAGQSQRRRPRKRTRNRTRSGQSSPSPD"
     gene            4303..4983
                     /locus_tag="VH419_14340"
     CDS             4303..4983
                     /locus_tag="VH419_14340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like fold-containing protein"
                     /protein_id="HEY7044843.1"
                     /translation="MTATPFADPAYRVAIVDLLGGLAYSELTAFERMTEDASRAPTLQ
                     DKVALASMATVEFGHFTRLRDRLIELEADPFEAMEPFHAPVDAFHAHTAPSDWLESLI
                     KAYVGDGLAADFYREIAAYVDESTRDLILATLADTGQADFAVEHVRRAIEEDPKVAGR
                     LALWARRLMGEALSQAQRVAAERDALSALLVGGVDRPGMDLAAIGRMFARLSETHTER
                     MAALGLRA"
     gene            complement(5006..5278)
                     /locus_tag="VH419_14345"
     CDS             complement(5006..5278)
                     /locus_tag="VH419_14345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020576402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEY7044844.1"
                     /translation="MELIGLIIGGVVIGLLGKLVAPKGKDDIPWWGVIVCGVGGILIG
                     DIIYVAFGGNGSPGIDWTRWIVAVLVSAGLVVIASAMFGRRTTTKV"
     gene            complement(5381..6049)
                     /locus_tag="VH419_14350"
     CDS             complement(5381..6049)
                     /locus_tag="VH419_14350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017934879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY7044845.1"
                     /translation="MLYAAIRLIISPLARLIFRPTIEGRDNVPKHGGVIIASNHLSFI
                     DSVLIPIVAPRPVVFIAKKEYWTGTGIKGALSRAWFTAMQSIPIERDTHGAAQASLDA
                     ALSVLKNGQAFGIYPEGTRSRDGRLYRGKTGVGWLALTAQVPVVPCAVIGTDKLLPIG
                     AKFPRFADVRVRFGTPLTFPPPGTGTTAAQARRAATDEVMAAIAAMSPQELAGVYNIA
                     PQSS"
     gene            complement(6110..6337)
                     /locus_tag="VH419_14355"
     CDS             complement(6110..6337)
                     /locus_tag="VH419_14355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007077129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3107 domain-containing protein"
                     /protein_id="HEY7044846.1"
                     /translation="MEVKIGVINANRELTLDTNQSVDDVRKAITDALSSERGLVSFAD
                     DKGRTVVVPVEKLAYVEISGESGRRVGFGSA"
     gene            complement(6393..7013)
                     /locus_tag="VH419_14360"
     CDS             complement(6393..7013)
                     /locus_tag="VH419_14360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020580200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY7044847.1"
                     /translation="MPTETGERPRTGRMPRSARRAQLLESALDVFVAQGYHAAAMDDI
                     AERAGVSKPVLYQHFPGKLELYLALLDTACDQVVHEVRAALASTTDNKARVAATTQAF
                     YAYVANAGGAFRLVFESDLTNEPAVRERVDRVTFLCADAIADVIADDTGFPPDSSRLL
                     AVALVGMSQVSARYWLSSEGELPQHHATDLISSLAWRGIRGFPRHE"
BASE COUNT         1063 a         2393 c         2467 g         1151 t
ORIGIN      
        1 cggcgtgtcg aacgccgatt gccacctcgt caggacggat tgccgccaca cccgtcacca
       61 gaccaccaaa cgagtgtccc tccagatgga cgcgccgact cggtggtcga gcggcgtacc
      121 gaaagtccct ctccagcagc ccgcgccacg tcatcgaccg cgcctggcga cccgttcgac
      181 gagcccggac ggcgtaagcc gcgacaccgc catgagggcc ttccagcgga agctgggaat
      241 cgagatggcc ttgccggcgc gaagatcggc gagcgcttgg ccgaccacgt cgtcggcctg
      301 cagccacatg aagccgggga tgccggaaat gttggcgtcc atccgttggt ggaactccgt
      361 ccggacgaac cccgggcaca cagcaaccac ccgcacgcct tgccgcttgt agcgcaggtg
      421 cgcccaccgg ctgaagctcg tcacccaggc cttgtgcgcg ccgtaggtgc ctcggggcac
      481 gaaacccgcc acgctcgaga cgttgacgat gtccccgtga ccgcgttcga gcatcgggcc
      541 gagggcggcg tgggtcagcc gcatcacggc acggaccagg acgtcgaagc tctcctgctc
      601 ggactcgatg tcgttggcct cgaacggttt ccgcttgccg aacccggcgt tgttcacgag
      661 tacgtcgacc gggtggtcgg cgagccacgc ctcgaccacc ttcgtctggt cgaggtcggc
      721 caggtccgcg gcgatgacgt cgcaggcaac gccgtgccgc tcggcgagct cggcggccac
      781 cttgtccagg cgcgtgcggt cacgggacac gagcatcagg tcgtggcctt cggcggcgag
      841 ggcttgcgcg aacccgtgac cgatcccgga ggtcggtccg gtgacgaggg cgagcggcac
      901 gcggacagcc ttgcacgctg gctcgctgac tgcgtgtcca ggccgggtga cggagcgaga
      961 cgccctaggc cgggtggcag aatgacggcc gatgacaacg acgctcgcga tcgccaacca
     1021 gaagggcggc gtcgccaaga ccaccaccgt cgcctcactg ggtaccgcgc tggtcgacca
     1081 gggtcttcgc gtcctgctcg tcgacctcga tccgcagtcg tgcctgacgt tctcactcgg
     1141 gatcgatccc gaggagctgg acgcctcgct gcaccaggtg ctgatgggcc agtcgaagtg
     1201 tgccgaggtg atcctggaga cggaggacgg gccagatctg ctgcccgcga cgatcgagct
     1261 cgccacggcc gagctgatgc tgctcacccg gccgggccgg gaacacgtcc tgcaagccgc
     1321 gctcgaggac gtcgacgccg actacgactg ggtgctgctc gactgcccgc cgtcgttggg
     1381 cgtgctcacg gtgtccgcgt tgtcggccgc cgactccgtg atgatcccgc ttcagtgcga
     1441 gaccctctcc catcgaggcg tgggtcagct gctcgacacg gtgcacgacg tccgccggtt
     1501 cacgaatccg cggctgcggg tgatgggcgt gctgccgacc ctgtacgact cgcgtccgaa
     1561 acattcgcgg gcggtgctcg agacgatctc ggagacgtat gacgtcgacg tcatcgagcc
     1621 gccgatcccg cgctcgatcc ggttcgccga ggcacctgct gccggtcgtt cgatcttccg
     1681 caccgcccgg tcgacgcggg gcgcgcaggc gtaccggacg ctggccgaaa acctcgccaa
     1741 cccgaccggc gcgccacgcg gaagcgaggc gcaatcggac gaatccacct gatcgactcc
     1801 tagacttccg tcgtgccttc acggcgagaa cctctgcggc gtacccgcac gccggccgga
     1861 cggatggtcg ctgtcggctc cgccgccgcg atcagcctgc tcacgatggc cggcgccttg
     1921 ggcttcggcc cgggagccgc gatggcacag acgcaacgga ctccctcgtc ggcggcgacc
     1981 gactcggctc ccgatcacca gagcgccgat cgcgacgcgg cagacccgtc ggttcacgcc
     2041 actctcgacg acggttggct gacgtacgac gactcgatgt ccaaggcccc cgccgcccag
     2101 cccgacgaga cggccctgcc accgaggacc ggccgcggcg agcgcatcgt gtacgacatc
     2161 agcgcgcaac gcgtgtggct tgtctcgtcc gacgactcgg tggagcggac gtacctcgtg
     2221 tcgggcggca aggacgagag cctcctcgag ccgggccgct accacgtgta ctcgatgtca
     2281 ccgcacgcgg tcagcttcaa ccacaaggag acgatgaact acatggtccg gttcgccacc
     2341 ggccaacatg cgccgatcgg gttccacgac gtgccggcgc ggctcgacgg cacattggtc
     2401 gagtcccgct ccgagctcgg tacgccgcag tcgtccggct gcatccggca gtggatcgac
     2461 gacgcccgcg cactgtggga gttcgcggaa atagacaccc cggtcgtggt caccgcctga
     2521 cgacgtcacg acgctggcca ccgaggtggc aatcgagtag cgacacgccg tcagaattca
     2581 acgaaagctg ccacctcggc gtaggtcagt cgggggatgg ggaggattgg ccggagcggg
     2641 tgcggttgcg ggtgcgtttg cgtgggcggc ggcgttgcga ctgcccggcg ccctgctcgc
     2701 tcgccgcggt tgcagactgc gcgggagacg tctccggcgt accggcgacg gcctgacccg
     2761 agcgggtacg acgtcgggag cggctgcggc tacggccggt cgacgcgccg tcagtggacc
     2821 tgtcgccgcg cgaaccgtcc ccacggctgc gagcgtcgcg gcgtccaccg cctgagcgcc
     2881 gatcccctcg acgcgagttc tccagcaggt ctgctgcgtc cggcttctcc cggggcttgc
     2941 cgatccggcc cttggcatcg ccggggatgc cttggtcgtg gtagaggtgc tcgctggtcg
     3001 agtacgtctc ctgcggttcg tcgaacggca ggtcgagcgc cttgttgatg accttccacc
     3061 gggtgacgtc cgcccagtcc acgaacgtga tcgcgacacc cgttgcgccc gcgcgtccgg
     3121 tgcggccgat ccggtggacg taggtgttct cgtcctcagg acatgtgtgg ttgatgacat
     3181 gggtgattcc cacgacgtcg atcccgcgcg cggccacgtc ggtggccacc aggatctcga
     3241 ccttgccgtc gcggaaacgc ttcatcgcgc gttcgcgagc cacctgggcc atgtcaccgt
     3301 gcaacggcgc tgcggggaag ccgcgttcga tcagctcctc agcgacccgc tgtgcctgcc
     3361 gtttggtccg ggtgaagatc agcgacaggc cacggttctc ggcctgcatg atccgggcga
     3421 tgacctcgat cttgtcgagg tcgtgcgcct gatagacgaa ctgggctgtg gcgggcactg
     3481 tggccctgtc gtcgctcgac tcggcccgga tgttgaccgg gtgccgcatg tgcgtacgcg
     3541 ccagcgtcac gacttcgccc cgcatcgtgg cggagaacag catcgtctgg cgcagctcag
     3601 gagtcttggc gatgagcttc tccacgtccg ggaggaaccc gaggtcgagc atctcgtctg
     3661 cttcgtcgag aacgagcact ttgacgtgcg ccaggtcgag ggccttgcgg gcctcgaggt
     3721 ccagcaaccg gccgggtgtg ccgacgacca cgtcgacgcc ggcggacaac gcggccagct
     3781 gcggttcgta gggcacgccg ccgtacacgg tgacggtgcg ggtgccccgg tgcttgccgg
     3841 ccaggctgag gtcgttggtg acctggagcg cgagctcgcg agtgggcgcg atgacgaggg
     3901 cttgcggctt gccgggtgcg gcgagctcgt cgtagtcggg gtcgtggggg gagacgatgc
     3961 gctgcaggat cgggatgctg aaggcaagcg tcttgccggt gccggtacgc gcctggccga
     4021 tgaggtcggt gcccatcaag gcgatcggga gcgtcatctc ctggatcggg aatgcgtcgg
     4081 tgatgtcgtc ctcggcgagc gcctcggcaa tctcggggag caccccgagc tcatggaacg
     4141 ttgtgatgat cgaactcgat tctgaggtcc tgtgcggtcg cgaggcctct ggcgcggcgc
     4201 cacgatggcc tcgtccgcgg ggcacgacgg cctcctccat cgggggcgtc gcgccagtaa
     4261 gtctatcggt tcggcgccgg ccgatcgcta cgctctctgt ccatgaccgc gacgccgttc
     4321 gccgaccctg cataccgggt cgccatcgtg gacctgctcg gcggcctcgc ctactcagag
     4381 ctcaccgcct tcgagcggat gacggaggac gcgtcccggg cgcccacgtt gcaggacaag
     4441 gtggccctcg cgtcgatggc gacggtggag ttcggtcact tcacccggct ccgtgaccgg
     4501 ctgatcgagc tcgaggccga cccgttcgag gcgatggagc ccttccatgc tccggtcgac
     4561 gccttccacg ctcacacggc gccctcggac tggctcgaga gcctcatcaa ggcgtacgtc
     4621 ggagacggtc tcgctgccga cttctaccgg gagatcgcgg cctacgtgga cgagtcgacc
     4681 cgcgatctca tcctcgccac gctcgccgac accggccagg cggacttcgc cgtggaacac
     4741 gtccgcaggg ccatcgagga ggacccgaag gtcgccggcc ggctggcgct gtgggcgcgg
     4801 cggctgatgg gggaggcact cagccaggcc cagcgggtcg cggccgaacg cgatgcgctg
     4861 tcggcgctgt tggtgggtgg cgtcgaccgg cctggcatgg acctggccgc gatcggccgg
     4921 atgttcgcca ggctgtccga gacccacacc gaacggatgg ctgccctcgg tctgcgcgcc
     4981 tgacccgcct cggcgtgggt gtacgtcaga ccttggtcgt cgtacgtcgt ccgaacatgg
     5041 ccgacgcgat cacgacgaga cccgcggaga cgagcaccgc gacgatccag cgcgtccagt
     5101 cgatgccggg actgccgttg ccgccgaacg ccacgtagat gatgtcgccg atcaggatgc
     5161 cgccgacgcc gcacacgatg acgccccacc acggaatgtc gtccttgccc ttcggtgcga
     5221 cgagcttgcc gagcaggccg atgacgacgc ctccgatgat cagcccgatg agttccatgc
     5281 cgactccttc cgacacgtga cgtcgggcaa cgcagcccga gaggtcgctg caccccagtg
     5341 tggcgcgcgt ggcctgcgaa ttcacgcgac ggttcgcgcg tcaggacgac tgtggcgcga
     5401 tgttgtagac gcccgcgagc tcctgcggtg acatcgccgc gatggcggcc atcacctcgt
     5461 cggtcgcggc gcgccgggcc tgcgccgcgg tcgtgccggt tccgggcggg gggaacgtca
     5521 gcggcgtacc gaatcggacc ctgacatcgg cgaagcgcgg gaacttcgcg ccgatcggca
     5581 acagcttgtc ggttccgatc accgcgcagg gaacaaccgg cacctgcgcg gtcagcgcga
     5641 gccaaccgac accggtcttg ccgcggtaga gacggccgtc gcgtgaacgc gtgccttccg
     5701 ggtagatgcc gaaggcctgg ccgttcttga gcacggacaa cgctgcgtcg agcgatgcct
     5761 gggcggcccc gtgggtgtcg cgctcgatgg ggatcgactg catggcggtg aaccaggcgc
     5821 gcgacagcgc acccttgatc cccgtcccgg tccagtactc cttcttggcg atgaacacca
     5881 ccggacgggg cgccacgatc gggatgagca cggagtcgat gaaggacagg tggttggacg
     5941 cgatgatgac gccaccgtgc ttcgggacgt tgtcgcggcc ctcgatcgtc ggccggaaga
     6001 tcaggcgcgc caacggcgag atgatgagcc ggatggcggc gtacaacacg gctggcctcc
     6061 tttgcggccg ggatcggtca gccgtgtcgc atggccgacc cgtggttggt caggcggatc
     6121 cgaagccgac cctccgaccg gattcgccgc tgatctcgac gtaggcgagc ttctcgacag
     6181 gtacgacgac ggtgcgaccc ttgtcgtcgg cgaaggagac gagtccgcgt tcggaggaca
     6241 gcgcgtcggt gatggccttg cgtacgtcgt cgacgctctg gttcgtgtcg agcgtgagct
     6301 cacggttggc gttgatgacg ccgatcttga cctccacgtg gagtcctttc atgtgtctcg
     6361 tgacgtgccg ctggactgtc tcgacgtcgg ggctactcgt gtcgggggaa cccgcggatg
     6421 ccgcgccagg caaggctgga gatcagatcg gtcgcgtggt gttgcggcag ctctccttcg
     6481 gagctcaacc agtagcgcgc cgacacctgg ctcatcccga cgagcgcgac cgcgagcagc
     6541 cgggacgagt ccggcgggaa gcccgtgtcg tctgcgatca cgtcggcgat cgcgtcggcg
     6601 cacaggaacg tcacccggtc cacccgctcg cgcaccgcgg gctcgttggt caggtcggac
     6661 tcgaagacga gacggaaggc cccgccggcg ttggcgacgt acgcgtagaa ggcctgcgtg
     6721 gtggcggcga cccgcgcctt gttgtcggtg gtgctggcaa gcgccgcgcg tacctcgtgc
     6781 acgacctggt cacaggcggt gtcgagcagg gcgaggtaca gctcgagctt gccggggaag
     6841 tgctggtaga gcaccggctt ggagacaccg gcgcgctccg cgatgtcgtc catggccgcc
     6901 gcgtggtagc cctgcgcgac gaacacgtcg agcgcggact ccagcagctg cgctctgcgt
     6961 gcgctgcggg gcatgcgtcc cgtccgcggt cgttctccgg tctcggtggg cactcgcgca
     7021 tcctaccgcc gagtcacttg gccaccgcca gctcacgcag gatcggctgg gccc
//
LOCUS       DATBFZ010000469         2567 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_4261680, whole genome shotgun sequence.
ACCESSION   DATBFZ010000469 DATBFZ010000000
VERSION     DATBFZ010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4261680"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(159..533)
                     /locus_tag="VH419_14365"
     CDS             complement(159..533)
                     /locus_tag="VH419_14365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017934706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEY7044848.1"
                     /translation="MPRFMGFVRMEEGVGTPPQALFDAMDVHIGERAAKGVFLDGGGL
                     YGTEDAVNFVVRQGQITRVDGPYAEAKEVVGGWAIMQYDTLEEAVADQQEFAELHAKY
                     WPEVTVISTLRQISTGPEAPGE"
     gene            complement(682..2151)
                     /locus_tag="VH419_14370"
     CDS             complement(682..2151)
                     /locus_tag="VH419_14370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY7044849.1"
                     /translation="MTTTPSITETVAVPTRTYPSLRAAWIPLAALCLAFFVEMVDNTL
                     LSIALPTIGRDLGSGTTALQWVTGAYSLTFGGLLLTAGSIADRFGRRRVLLVGLAAFG
                     LMSLCVIVVTTAGQLIALRAALGIAAAAMAPITNSLVFRLFDDEAVRRRAITVMIVVG
                     MSGFVLGPLLGGTALAHVRWEWLLVVNAPIALFACIGVRLGVPADRPEDLTDDTLDLP
                     GAVLSIMTIGLACYSLTSGVLHGWLSAITLASVVGALAAAVGFVWHERRTSAPMLDLG
                     LFSSGTVRGAAIAQAGTAIGMASVMFGLILHFQFAYGWSPVRAGLANLPIIVTMIAAT
                     PVSESLARRFGHRMACLVGAAFLTGSLAGLARAVDHGYVAIAVCMVLMTIGLRTVMTI
                     CAVALIDAMPSNRTSIGAALNDTAQEVGTSVGTAVIGTAIAALVTTRLPPGVWSSDLV
                     ASFFHGERVTYLLLAAVVGLVTVWGALTLSDSRATEEAG"
     gene            complement(2241..>2567)
                     /locus_tag="VH419_14375"
     CDS             complement(2241..>2567)
                     /locus_tag="VH419_14375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020387885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY7044850.1"
                     /translation="PHPERRAMTTTMVRPPSLDDLSSVGTRREEVIGRLSPRELEVLA
                     LMAEGCSNRAIGELLRVELKTVETHAGRVFSKLGLNEDRQENRRVLAVLAFLGRDDAS
                     ATTVCR"
BASE COUNT          419 a          870 c          907 g          371 t
ORIGIN      
        1 ctcgaccctc cgccagatct ccactggccc ggaggcgccc ggcgactgaa cgggactcac
       61 tacgctggcc tcgtgccggc agagtccaga ccggatgacc ccaccgaggt catcttcgcc
      121 gcgtggcggg ccgagtcggc ccgcctcgtg acctcagctc actcgccggg ggcctccgga
      181 cctgtcgaga tctggcgcag cgtggagatc acggtgacct cgggccagta cttcgcgtgc
      241 agctcggcga actcctgctg gtcggcgacg gcctcttcga gggtgtcgta ctgcatgatc
      301 gcccagccgc ccacgacctc cttggcctcg gcgtagggtc cgtcgacccg ggtgatctgg
      361 ccctggcgga ccacgaagtt cacggcgtcc tccgtgccgt acaggccgcc gccgtcgagg
      421 aacacaccct tggccgcacg ttcgccgatg tggacgtcca tcgcgtcgaa cagcgcctgg
      481 ggaggcgtac cgactccctc ttccatcctc acgaatccca tgaaacgagg catcctgatc
      541 actccttgtg gttgctggga cgtgctctct cgccacgtac gtcgaacggc gtccgggcac
      601 ttcgacatcg gtcccgacgt tttccgtcca tcctgccgaa gtgtctgcgg ccggtcggcg
      661 cggcccatca gcggacagac gtcagccggc ttcctcggtg gcgcgggagt cgctgagggt
      721 gagggctccc cacaccgtca cgaggccgac gacggctgcc agcaggaggt aggtcactcg
      781 ttcaccatgg aagaacgacg ccaccaggtc gctgctccag acgccgggtg gcagccgagt
      841 ggtgaccagt gcggcgatcg cggtgccgat gacggcggta ccgacgctgg tgccgacctc
      901 ctgcgcggtg tcgttgagcg cggcgccgat ggaggtgcgg ttgctcggca tcgcgtcgat
      961 cagcgccacc gcacagatcg tcatcacggt gcgcagcccg atggtcatca ggaccatgca
     1021 gacggcgatg gcgacgtacc cgtgatcgac cgcccgggcg aggccggcca gcgagccggt
     1081 gaggaaggcc gcgccgacga ggcaggccat gcggtgcccg aaccggcgtg cgagcgactc
     1141 cgacacgggg gtcgccgcga tcatggtcac gatgatcggc aggttcgcca gaccggcgcg
     1201 gaccgggctc cagccgtagg cgaactggaa gtggaggatc agcccgaaca tcacgctcgc
     1261 catcccgatc gccgtcccgg cctgggcgat ggccgcgcct ctgacggtgc cgctggagaa
     1321 gaggcccagg tccagcatcg gtgcggacgt gcggcgttcg tgccacacga acccgaccgc
     1381 ggcagcgagg gcgccgacga cggaggcgag ggtgatcgcg gagagccagc cgtgcagaac
     1441 gccgctggtg agggagtagc acgcgagacc gatcgtcatg atgctcagca cggcgcccgg
     1501 caggtcgagc gtgtcgtcgg tcaggtcctc cggccggtcg gccggcactc ccaggcgtac
     1561 gccgatgcag gcgaacagcg cgatcggcgc gttgacgacc agcagccact cccaccgcac
     1621 atgggccagc gcggtgccac cgagcagtgg cccgaggacg aagcccgaca tgccgaccac
     1681 gatcatcacg gtgatggcgc gccggcgtac cgcttcgtcg tcgaagagcc ggaagacgag
     1741 ggagttggtg atcggcgcca tcgctgcggc ggcgatgccc agggcggcgc gaagggcgat
     1801 gagctggccg gccgtggtga cgacgatgac gcacaggctc atcagcccga acgcggcgag
     1861 cccgaccagg agcacacggc ggcggccgaa ccggtccgcg atcgacccgg ccgtgagcag
     1921 cagcccgccg aacgtcagcg agtacgcccc ggtgacccac tggagtgccg tcgtgccact
     1981 gccgaggtcg cgaccgatgg tgggcagtgc gatcgacagc aaggtgttgt cgaccatctc
     2041 gacgaagaag gctagacaca gggcggccag cgggatccag gccgcacgca gcgatgggta
     2101 tgtgcgcgtc ggaaccgcga ccgtctcggt gatggacggg gtggtggtca tgcgggcctc
     2161 ctggttgacg gcgtacgcgt gctggacgac gcatgaccga ccgtaggaag cagcgctttc
     2221 accgcggttt catcgtggct tcaccggcag acggtcgtgg ccgacgcgtc gtcgcgaccg
     2281 aggaacgcga ggaccgcgag gacccgccgg ttctcctgtc ggtcctcgtt gaggccgagc
     2341 ttggagaaga cgcggccggc atgcgtctcg accgtcttga gctccacccg gagcagctcg
     2401 ccgatggcgc ggttcgagca gccctccgcc atgagcgcga gcacctcgag ctcgcgcggg
     2461 gacagtcgcc cgatcacctc ctcacgacgc gttcccacgg atgagagatc gtcgaggctc
     2521 ggaggccgga ccatcgtggt cgtcatcgct cgtcgctccg ggtgagg
//
LOCUS       DATBFZ010000470         2104 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_6074821, whole genome shotgun sequence.
ACCESSION   DATBFZ010000470 DATBFZ010000000
VERSION     DATBFZ010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6074821"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..771)
                     /locus_tag="VH419_14380"
     CDS             complement(<1..771)
                     /locus_tag="VH419_14380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020580257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HEY7044851.1"
                     /translation="MSATMRTPDGETLELDVAGVTEGAPGADISKLLSKTGLVTFDPG
                     FANTASCTSQITYIDGDAGILRYRGYPIDQLAEGSTFLETSYLLIYGELPTADQLSSF
                     EGQIRRHTLLHEELKRFFDGFPRDAHPMPVLSSAVSALSTFYQDALDPFNEQDVELST
                     VRLLAKLPTIAAYAHKKSVGQPFLYPDNSLGLVENFLRMTFGFPAEPYEADPAVVRAL
                     DLLFILHADHEQNCSTSTVRLVGSAQANLFASISAGINA"
     gene            complement(789..1226)
                     /locus_tag="VH419_14385"
     CDS             complement(789..1226)
                     /locus_tag="VH419_14385"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007079226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEY7044852.1"
                     /translation="MAHLDLTDLPTAMPGLDGHLGIDLVEASGDRVILRWTVRPEHTQ
                     AYGIVHGGTYCTAVESSASVGAALWFGGRGRVVGVSNQTDFYRSVTAGELTATAVPLH
                     RGRSQQVWRVTIDDEQGRMVAQGQVRLQNLTDPDRGPGHAATD"
     gene            1309..2034
                     /locus_tag="VH419_14390"
     CDS             1309..2034
                     /locus_tag="VH419_14390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020391288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase family hydrolase"
                     /protein_id="HEY7044853.1"
                     /translation="MLGLPDTVTACLFDLDGVLTDTASVHRKAWKQTFDLLLATRGQA
                     EFTDQDYLAYVDGKPRADGVRDFLASRGITLPEGADDDPPGDTTVNAVGNRKNVALLK
                     AIHDDGVLVFADARAYLDASREAGLRRLVVSSSANTKDVLHVTGLEALVEGRVDGVTI
                     KEKKLRGKPAPDSFLTGAEVLGVRAEQAAVFEDALSGVQAGRDGGFGFVVGVNRQDDA
                     HGRGLAANGASVVVSRLTELLGP"
BASE COUNT          343 a          703 c          731 g          327 t
ORIGIN      
        1 cgcgttgatg cccgccgaga tcgacgcgaa gaggttggcc tgcgccgagc cgacgaggcg
       61 cacggtcgac gtcgagcagt tctgctcgtg gtccgcgtgc aggatgaaca gcaggtccag
      121 cgcgcgcacg acggccgggt ccgcctcgta cggctcggcc gggaagccga acgtcatgcg
      181 caggaagttc tccaccaggc cgagcgagtt gtccgggtag aggaacggct ggccgacgga
      241 cttcttgtgg gcgtacgccg cgatcgtggg cagcttcgcc agcaaccgga cggtggacag
      301 ctccacgtcc tgttcgttga acggatcgag cgcgtcctgg tagaacgtcg acagcgccga
      361 caccgcggac gagagcaccg gcatcgggtg cgcgtcacgc gggaagccgt cgaagaaccg
      421 cttcagctcc tcgtgcagca acgtgtggcg acggatctgg ccttcgaagg acgacagctg
      481 gtcggcggtg ggcagctcgc cgtagatcag cagatagctc gtctcaagga aggtcgaccc
      541 ttcggcgagc tggtcgatcg gatagccccg atagcgcagg atccccgcgt caccgtcgat
      601 gtaggtgatc tgcgacgtgc acgacgccgt gttcgcgaag ccggggtcga aggtgaccag
      661 tccggtcttc gacagcagct tgctgatgtc ggcacccggg gctccctcgg tgacgcccgc
      721 gacgtccagt tccagtgttt ccccgtccgg agtgcgcatc gttgctgaca taggggtctc
      781 cctgtcgttc agtcggtggc cgcgtgcccc ggaccccggt cgggatcggt gaggttctgc
      841 agccgtacct gtccttgtgc caccatgcgg ccctgctcgt catcgattgt gactcgccag
      901 acctgctgac tgcggcctcg atgcaacggc accgcggtcg cggtcagttc gccggcggtg
      961 acggaccggt agaagtcggt ctggttgctg acgccgacca cccgtccacg cccgccgaac
     1021 cagagcgcgg cccccacact ggccgatgat tccaccgcag tgcaatacgt cccgccatgc
     1081 acgatcccgt acgcctgggt gtgctcggga cgcaccgtcc agcgcaggat cacacgatca
     1141 ccggacgcct cgaccagatc tatcccgagg tgaccgtcca gccccggcat cgcggtcggc
     1201 agatcggtca ggtcgaggtg cgccacaact cgaacctagg ccaccggcgc ggcgcatcac
     1261 ccggcgggcg gcgcgacggg cggcaggctg cgggtagatg ctgtaccaat gctcggactg
     1321 cccgacaccg tgacggcctg cctgttcgac ctggacggtg tcctgaccga cactgcttct
     1381 gtgcaccgca aggcgtggaa gcagaccttc gacctgctgc tcgccacgcg cggacaggcg
     1441 gagttcaccg accaggacta cctcgcctac gtcgacggca agccccgcgc cgacggcgtg
     1501 cgcgacttcc tcgcctcgcg agggatcacg ctgcccgagg gcgcggacga cgacccgccc
     1561 ggtgacacga ccgtcaacgc cgtgggcaac cgcaagaatg tcgcgctgct gaaggcgatt
     1621 cacgacgacg gcgtgctcgt cttcgcagat gcgcgcgcgt acctcgatgc gtcccgggag
     1681 gccggcctgc gccgcctcgt cgtgtcctcg agcgcgaaca ccaaggacgt gctgcatgtg
     1741 accggccttg aggcgctggt cgaagggcgc gtcgacggag tgacgatcaa agagaagaag
     1801 ctgcgcggga agccggcacc ggactcgttc ctgacgggcg cggaggtact cggcgtgcgt
     1861 gcggagcagg cggccgtgtt cgaggacgca ctgtccggcg tgcaggccgg ccgcgacggc
     1921 ggcttcggct tcgtcgtcgg cgtcaaccgc caggatgacg cacacgggcg ggggctcgcc
     1981 gccaacggcg cctcggtcgt cgtgtcgcgg ctgaccgagc tgctcggacc gtgacgggac
     2041 tctgacggga ggaaacgtga cgacgaggca tgccgaccgc tatccgatcg agccgtggga
     2101 gctg
//
LOCUS       DATBFZ010000471         6774 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_1968656, whole genome shotgun sequence.
ACCESSION   DATBFZ010000471 DATBFZ010000000
VERSION     DATBFZ010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 6774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6774
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1968656"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="VH419_14395"
     CDS             complement(<1..323)
                     /locus_tag="VH419_14395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015069869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HEY7044854.1"
                     /translation="MTTESSASTAETYGNALVVEQNGLNVIPESERKGQPRDLFWPWC
                     ASNISVFGISWGAFVIGFGLSLWQALVAGVVGVVASFLLVGLVALAGKRGSAPTLVLS
                     RAPFG"
     gene            complement(475..1818)
                     /locus_tag="VH419_14400"
     CDS             complement(475..1818)
                     /locus_tag="VH419_14400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044855.1"
                     /translation="MALRHTPNVPRCRLTMALLRAAVIGGLIAATLAGCDSLGHTATD
                     VQRQGATSEPNSSPAGPAYQAVPGDFAAIRTLLKDRAHALTVGDRAAFIATVDPVSDA
                     FVERQNTYFDNLQALPVSVVTYEVADYALIPAPVRGGDPVLRPDVSEHVFMPGTDTRP
                     VGTGVGYTFVKRNGQWLLGAVDNSTAEFSGGAETAQPWSGGAISVEVRHHLVVVTDEA
                     AAGTTASLADAVAGDITRVARIVHQPADTRLLVDATSVGEATRISAIGQEDAAAITYP
                     VAATRDNEVTGLAGWRIKINPDRTQDLMLEPWLLRHEVAHYVLRRVSSCTPLWVSEGI
                     AEYAGEWPSRFSDQSITPSTYAAVQNAPRALPSSPMWSNDPGINYLIAHAAVTFLVRQ
                     YGMDRMIDLMTAYSTCRGDDSTGDANTSGLLKQTFGVTEAEVVDGAFDLIAQLRH"
     gene            1895..2983
                     /locus_tag="VH419_14405"
     CDS             1895..2983
                     /locus_tag="VH419_14405"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family peptidase"
                     /protein_id="HEY7044856.1"
                     /translation="MTYPLIRATGTPEEIGASYGEQAAELIAGNLEDYRTKFAAVGLG
                     ASDVTRIGDQFAATTREYTPRIAAMLDAVAESSGTAVGDVYALNARTELMLPDGLRIT
                     ECTSVAVLPEHTATGNTLLAQNWDWHPSQRPFMVVLATRDERGHEVVTLAEAGMLAKA
                     GLSSAGLGICVNLLHSDRDGAPGGVPYHVLIRHVLDQPGGLAYAMRVTALPRSASINL
                     LIAAAGGTAVDLEVAPGMVGRITPQDGLLTHANHFVSGIPLVDAIYDTGGSSLFRDYR
                     MRQLLAVAGADRKVLVDDVIAALQDHLGLPYAICRHVDEEDPENLKSMSAASVVMDLD
                     ERVMLVASGPPCETAYEHIDLSTVFDSA"
     gene            complement(3041..3985)
                     /locus_tag="VH419_14410"
     CDS             complement(3041..3985)
                     /locus_tag="VH419_14410"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HEY7044857.1"
                     /translation="MFVVLGEALLDMVQPAPGTTYDARPGGGPFNIAVGLARLGHPTA
                     LLARLSTGGLGRILRDHATANGLDLSACVDTPSQTTLAFASLDDHGRATYDFYVQGTA
                     DWGWTPGELATLPPDMRVFHTGSLATVVDPGADVVLDRLERLHATGQVLLSFDPNVRP
                     VLAGDRDAAVRRVERFVAAAHVVKASEEDLEWLYPGLDTVDAARRWAAHGPALVVVTR
                     GAEGCVAVTRVGSVLERRAPAVDLVDTIGAGDAFESGLLSGLVDAGAVDAASVAALPN
                     SALGKALDRAVLVAGMTCERLGADPPTRAEYEACAPVD"
     gene            complement(4002..5987)
                     /locus_tag="VH419_14415"
     CDS             complement(4002..5987)
                     /locus_tag="VH419_14415"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012918870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HEY7044858.1"
                     /translation="MSQPPSGSIPGVTGIDTSIRPQDDLFGHVNGEWLATAEIPADLP
                     TAGVFIDLVLDAERRTADILHDAAERSAAGDLAPGSPQKKIGDIFASFMDEARVEELG
                     AAPLSDDLNAIEAVSTTAQLFELLGRLERDGVSGVVSAYVDTDDRNSDRYVVKMVQAG
                     IGLPDESYYRDDGFADVRTKYVAHVARMLELAGRTSQQAADEAERIMALETRLASGHW
                     DRVASRDVVKTYNLMSRTELAAAAPIVDWDTWIDALQAPPAAFAEVVVRQPSYLSTVS
                     DALADLPLDDWKAWLRWQLVHAAAPYLSDAFVRENFEFYSRTLAGSEELRERWKRGVA
                     LAELALGEAVGQEYVARHFPPESKAAMDELVANLVEAYRRNIEKLEWMGQATRERALE
                     KLRAFRPKIGYPVRWRDYSALEIDRDDLLGNVRRAIAFETDRQLAKIGKPVDRDEWLM
                     TPQTVNAYYNPGTNEICFPAAILRAPFFDIEADPALNYGGIGAVIGHEVGHAFDDQGS
                     QYDSSGNLSNWWTDDDRSRFTERAETLIKQYDAFEPRDLPGHKVNGALTVGENIGDLG
                     GLAIALHAYEISLDGTQPSVVQGLSARQRLFANWATVWRSKRREQYQLQLLSVDVHSP
                     PEFRANIVRNLDEFHEAYQTKPGDALWLDPEDRVRIW"
     gene            6068..6649
                     /locus_tag="VH419_14420"
     CDS             6068..6649
                     /locus_tag="VH419_14420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044859.1"
                     /translation="MTAKAFAAAVFVATVVQLAIGALVPGLEQFDGKAFGARLIAYPL
                     LMLAAPVTWWVVRRRRGSSAPMPWDGFALIMAPFLVDVTGNALDLYDSVSWWDDANHF
                     VNWALLCAGFGLLIVRGGVRPPWAFVLAVTGLGALLAVGWEIGEWYTFIRHGTELDTA
                     YEDTLVDETLGTLGGLLGTVLVAWQKRSVLRPT"
BASE COUNT         1038 a         2378 c         2267 g         1091 t
ORIGIN      
        1 attccgaagg gagcccgcga cagcaccagg gtcggtgcgg acccgcgctt gccggcgagc
       61 gccaccaggc cgacgagcag gaacgacgcg acgacgccga cgacgcccgc gaccagcgcc
      121 tgccacaggc tcaggccgaa cccgatgacg aacgcgcccc agctgatccc gaacaccgag
      181 atgttcgagg cacaccacgg ccagaacagg tcacgcggtt ggcccttgcg ttccgactcg
      241 gggatgacgt tcagcccgtt ctgctcgacg accaacgcgt tgccgtaggt ctcggcggtg
      301 gatgcggaac tctccgtggt catgacgcgc ctctcgttgc cccgatgtgg tgctccaggt
      361 ggaacctagt ctccaggcca cccggcggtc gagcctgtcg aggccccgga tacgggcgtg
      421 cccttgatct cgacagtgat gatcagcggg aggtccggcg accggcgggc tttgtcagtg
      481 gcggagctgg gcgatcaggt cgaaggcgcc gtccaccacc tcggcctcgg tgacaccgaa
      541 ggtctgcttc aacagtcccg acgtgttcgc gtcaccggtg ctgtcgtctc cccgacacgt
      601 gctgtacgcc gtcatcaggt cgatcatccg gtccatgccg tactgccgga cgagaaaggt
      661 gactgcggcg tgcgcgatca ggtagttgat gcccgggtcg ttgctccaca tcggtgagct
      721 cggcagcgct cgaggggcgt tctgcaccgc ggcgtacgtc gacggcgtga tcgactggtc
      781 gctgaaccgc gaaggccact cgccggcgta ctccgcgatg ccctccgaga cccacagcgg
      841 cgtacagctg ctgacccggc gtagcacgta gtgagccacc tcatggcgca gcagccacgg
      901 ctccaacatc aggtcctggg tgcggtccgg gttgatcttg atgcgccaac ccgccaagcc
      961 ggtcacctcg ttgtcacgcg tcgccgccac cgggtaggtg atggccgccg catcctcctg
     1021 gccgatcgcg ctgatccggg tcgcctcgcc gacagacgtt gcgtccacca gcaaccgggt
     1081 gtccgcgggc tggtggacga tgcgggccac ccgagtgatg tcgcctgcga ccgcgtcggc
     1141 cagcgaagct gtcgttccgg ccgccgcctc gtcggtcacg acaaccaggt ggtggcgtac
     1201 ctccacgctg atcgccccac ccgaccatgg ttgggcggtt tcggcaccgc cgctgaactc
     1261 cgcggtcgag ttgtcgacag ctcccagaag ccactgcccg ttccgcttca cgaacgtgta
     1321 gccgacaccg gtcccgacgg gtcgggtgtc cgtaccaggc atgaacacgt gttcgctgac
     1381 gtccgggcgc agcacgggat cgccgccccg gaccggcgcc gggatgaggg cgtagtccgc
     1441 cacctcgtaa gtgacgacag agacgggaag cgcctgcagg ttgtcgaagt acgtgttctg
     1501 ccgttccacg aacgcgtcac tcaccgggtc gacggtggcg atgaacgccg cccgatcgcc
     1561 gacggtcaag gcgtgcgcgc ggtctttcag cagcgtccgg atcgccgcga agtcgccggg
     1621 caccgcctgg tacgcaggtc cggccggcga ggaattcggc tcacttgtgg cgccctgcct
     1681 ttggacgtcc gtcgccgtgt ggccgaggct gtcgcagccg gccaacgtgg ctgcgatgag
     1741 gccgccgatc acggccgctc gcagcagggc catcgtcagg cggcagcgcg ggacgttcgg
     1801 ggtatgacgc aacgccacgc gcgcaatata tccgcggctc gcctgtcaca gacctggaag
     1861 gcgaggaacg ggtcgccggg ctaccctcga cgccatgacc tacccgctga ttcgcgccac
     1921 cggcacaccc gaggagatcg gcgcctcgta cggagagcag gcggctgagc tcatcgccgg
     1981 caacctcgag gactaccgca cgaagttcgc agccgtgggc ctcggcgcca gcgacgtcac
     2041 ccggatcggc gaccagttcg cggcgaccac acgtgagtac accccgcgta tcgcggcgat
     2101 gctcgacgcc gtcgcggagt cgtccggcac agcggtcggc gacgtctacg cactgaacgc
     2161 gcgcaccgaa ctcatgcttc ctgacggcct gcgtatcacc gagtgcacca gcgtcgccgt
     2221 gctccccgag cacacggcga ccggcaacac cctgctcgca cagaactggg actggcaccc
     2281 gtcgcagcgg ccgttcatgg tcgtcctcgc cacccgcgac gagcgcggac acgaggtcgt
     2341 gacgctcgcc gaggcgggca tgctggccaa ggccggcctg agctccgccg gcctcggcat
     2401 ctgcgtcaac ctgctgcaca gcgaccggga cggcgcgccc ggcggagtgc cctaccacgt
     2461 gctgatccgg cacgtgctcg accagcccgg tggtctcgcg tacgcgatgc gcgtcacggc
     2521 cctcccccgc agtgcgtcga tcaacctgct gatcgcagcc gccggcggca cggcggtcga
     2581 cctggaagtc gcccccggca tggtcggccg gatcacgcca caggacggct tgctgacgca
     2641 cgccaaccat ttcgtgtccg ggatcccgct ggtggacgcc atctacgaca ccggcggctc
     2701 gtcgctgttc cgcgactacc ggatgcgtca gctgcttgcg gtcgcgggcg ccgaccgcaa
     2761 ggtcctggtc gacgacgtga tcgccgcgct gcaggaccac ctcggtctcc cctacgccat
     2821 ctgtcgccac gtcgacgagg aggaccccga gaacctcaag tcgatgtcag cggcgtcggt
     2881 cgtcatggac ctcgacgagc gggtgatgct cgtggcctcc ggccctccgt gcgagacggc
     2941 gtacgagcac atcgacctga gcacggtctt cgactccgcc tgaccgagtc gctgcgacga
     3001 ccgggtacct ggtttcgacg agcccaatca cccggctcgc tcagtcgacc ggtgcgcagg
     3061 cctcgtactc cgctcgggtc gggggatcgg cacccagccg ctcgcaggtc atgcccgcca
     3121 cgagcaccgc ccggtcgagc gccttgccca gcgcactgtt cggcagtgcg gccaccgaag
     3181 cggcgtccac ggcccccgcg tcgaccaggc cggacaacag accggactcg aacgcgtccc
     3241 cggcaccgat cgtgtcgacg aggtcaacag ccggggcgcg gcgctcgagc actgatccca
     3301 ccctggtcac ggccacacag ccctcggctc cccgggtcac gaccaccagc gctggaccat
     3361 gcgcggccca ccggcgtgcc gcgtcgacgg tgtcgagccc tggatacaac cactcgaggt
     3421 cttcctcgct ggccttgacc acgtgtgcag ccgcgacgaa gcgctcgacc cggcgtaccg
     3481 cggcgtcacg gtcgcctgcc aacacgggtc ggacgttcgg atcgaagctc agcagaacct
     3541 ggccggtcgc atgcagccgc tcgagccggt ccagcacgac gtcggcaccg ggatcgacga
     3601 cagtggcgag tgatccggtg tggaacaccc gcatgtctgg cggcagggtg gccagctcgc
     3661 ccggggtcca gccccagtcc gcggtcccct gaacgtagaa gtcgtacgtc gcccgaccgt
     3721 ggtcgtcgag cgacgcgaac gcgagcgtcg tctggctcgg ggtatcgacg cacgcgctga
     3781 gatccagacc gttcgccgtc gcgtggtcac gcaggatccg tccgaggccg cccgtcgaca
     3841 gccgcgcgag cagggcggtg ggatgaccga gtcgggcgag tccgacggcg atgttgaacg
     3901 ggccgccgcc tggtcgtgcg tcgtacgtcg ttcccggcgc cggctgcacc atgtcgagca
     3961 gcgcctcgcc gaggaccacg aacatcgctg acccctccgg tctaccagat gcggacgcgg
     4021 tcttcgggat cgagccagag cgcgtcgccg ggcttcgtct ggtacgcctc gtggaactcg
     4081 tcgaggttgc gcacgatgtt cgcccggaac tcgggaggtg agtgcacgtc caccgagagc
     4141 agctggagct ggtactgctc gcgccgcttg ctccgccaca cggttgccca gttcgcgaac
     4201 agccgctgcc gggccgacag tccctggacg accgacggct gtgtcccgtc gaggctgatc
     4261 tcgtacgcgt gcagagcgat ggcgagcccg ccgaggtcgc cgatgttctc gccgaccgtc
     4321 agcgcgccgt tcaccttgtg gccgggcagg tcgcgcggct cgaaggcgtc gtactgcttg
     4381 atcaatgtct ccgcacgctc ggtgaaccgg gatcggtcgt catcggtcca ccagttggag
     4441 aggttgccgg aactgtcgta ctgcgatccc tggtcgtcga aggcgtgtcc gacctcgtgc
     4501 ccgatcaccg cgccgatgcc gccgtagttg agtgccgggt cggcctcgat gtcgaagaac
     4561 ggcgcacgca ggatggccgc cgggaaacag atctcgttcg tcccggggtt gtagtaggca
     4621 ttgaccgtct gtggcgtcat cagccactcg tcgcggtcga ccggcttgcc gatcttcgcg
     4681 agctgtcggt cggtctcgaa cgcgatggcc cgtcgtacgt ttccgagcag gtcgtcccgg
     4741 tcgatctcga gcgccgagta gtcgcgccag cgcacggggt agccgatctt cggccggaag
     4801 gcgcgcaact tctccaaggc gcgctcgcgt gtcgcctgcc ccatccactc gagcttctcg
     4861 atgttgcggc ggtacgcctc gacgaggttc gcaaccagct cgtccatcgc cgccttggac
     4921 tccggaggga agtgtcgcgc gacgtactcc tgcccgaccg cctcgccgag tgccagctcg
     4981 gccagcgcca ccccgcgctt ccagcgttcc cgcagctcct cagatcccgc cagcgtgcgc
     5041 gagtagaact cgaagttctc ccggacgaag gcatccgaca ggtacggcgc cgccgcgtgc
     5101 acgagctgcc aacgcaacca cgccttccag tcgtcgagag ggaggtcagc gagagcgtcg
     5161 gagaccgtcg acaggtatga cggctgccgc accacgacct cggcgaatgc cgctggtggc
     5221 gcctgtagcg catcgatcca ggtgtcccag tcgacgatcg gcgcggccgc tgccagctcg
     5281 gtgcgcgaca tcaggttgta cgtcttcacg acgtcgcggc ttgccaccct gtcccagtgt
     5341 cctgaggcga gccgggtctc caacgccatg atgcgctcgg cctcgtcggc tgcctgctga
     5401 ctcgtccggc cggcgagctc gagcatccgg gccacgtgcg cgacgtactt cgtgcgtacg
     5461 tcggcaaaac cgtcgtcccg gtagtacgac tcgtccggca ggccgatccc ggcctggacc
     5521 atcttcacga cgtaacggtc ggagttcctg tcgtcggtgt cgacgtatgc cgacaccact
     5581 ccggacacgc cgtcccgctc gagccggccg agcagctcga acagctgcgc cgtcgtcgag
     5641 accgcctcga tcgcgttcag gtcgtcggac agcggcgcgg cacccagttc ctcgacgcga
     5701 gcctcgtcca tgaagctggc gaagatgtcg ccgatcttct tctgcggcga ccccggtgcg
     5761 aggtcgccgg cggcactgcg ctcagccgcg tcatgcaaga tgtcagcggt gcggcgctcc
     5821 gcgtcgagca cgaggtcgat gaagacaccg gctgtcggca ggtcggccgg aatctccgcg
     5881 gtcgccagcc attcgccgtt cacgtgaccg aacagatcgt cctgaggtct gatggacgtg
     5941 tcgatcccgg tgacacctgg aatggagccg gaaggtggtt gactcacgga agaccgcctc
     6001 ttcgatcgtc acgacactcg cgtgcgcggg tatccgcgac cctatctccg gaagcgaggg
     6061 gactgcgatg acggccaagg ccttcgcggc ggccgtcttc gttgccacgg tggtccagct
     6121 ggcgatcggc gccctcgtcc cgggtcttga gcagttcgac gggaaggcat tcggggcccg
     6181 gctgatcgcg taccccctgc tcatgctggc ggcgccggtg acgtggtggg tcgtacgacg
     6241 ccggcgcggc tcgtcggcac cgatgccgtg ggacgggttc gcgctcatca tggcgccgtt
     6301 cctcgtcgac gtcacgggga acgctctcga cctgtacgac agcgtctcct ggtgggacga
     6361 cgccaaccac ttcgtcaact gggcgctgct ctgcgccggc ttcggactgc tcatcgtgcg
     6421 cgggggcgtg cggccaccct gggcgttcgt gctggccgtc acggggttgg gcgcgctgct
     6481 ggccgtcggg tgggagatcg gcgagtggta cacgttcatc cggcacggta cggagctcga
     6541 cacggcgtac gaggacaccc tggtcgacga gactctgggc acactcggtg gtctgttggg
     6601 caccgttctc gtcgcgtggc agaagcgaag cgtcctgcgt ccgacgtagc gcacacggca
     6661 cagtgcgtga ccatcgcggt tctgcgcgca cggccgactg aggcggacga caatatggcc
     6721 cgtggaccat gagctccggc taccgagcga cacggagacg gtcccgctgg ctcg
//
LOCUS       DATBFZ010000472         2232 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_6075544, whole genome shotgun sequence.
ACCESSION   DATBFZ010000472 DATBFZ010000000
VERSION     DATBFZ010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6075544"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..885)
                     /locus_tag="VH419_14425"
     CDS             complement(<1..885)
                     /locus_tag="VH419_14425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044860.1"
                     /translation="MTEEDRAATIRSVERWFEHQGLPYFVPSNAERVHAALSRSRLIP
                     VLAVALALGVAAGLFSGLGSSDVSSGIVVGALVLGLVVLAYGLFSLHLKPIIRWATGQ
                     TFGSLGSLFPLVTRALPLLLLFITFLFINTEVWQVCSALDRGLLWLTVMLFAAIAVVF
                     LLVRLPEEVRQVSAEAEGDRLVKICARTPVEDAAHELTNDAAHADLTRLQQANLVLVL
                     LFSQALQVLLLSLSVFAFFLVFGKVAITDTVIKAWVGTAPEPLPSLDWLPISNQLFQV
                     AVFLAAFSGLYFTVYAVTD"
     gene            complement(1005..1652)
                     /locus_tag="VH419_14430"
     CDS             complement(1005..1652)
                     /locus_tag="VH419_14430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044861.1"
                     /translation="MLSRGKHRNPRRGACFMEFASWLAGEKFSDHPACTHPLLATVAR
                     GVNDNLSDAGRQRIVPLIADVVGLNGEDPVVDVTITVLAATKAFPVAAYDQQKGLAVG
                     LLRCEDEIDAMDDPRTKALMPMIEEALEHCPQATTWAQHFVARPWSGGNDFARARSHI
                     VRVAVMSLALAPASDDRLCELLETSVARTRMLLDTPRVDAPASWTAAPDSIDATA"
     gene            complement(1832..>2232)
                     /locus_tag="VH419_14435"
     CDS             complement(1832..>2232)
                     /locus_tag="VH419_14435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002519309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="riboflavin kinase"
                     /protein_id="HEY7044862.1"
                     /translation="VRGYVVKGDQRGRELGYPTANVPVQSSRTAVPADGVYAGWLRRL
                     DDPDAAYLPAAISVGTNPTFDGTDRRVESYVLDRDDLELYGVIVEVAFEERLRGMVKF
                     DSVDALLVQMADDVDRARAMLTTPPLIPAG"
BASE COUNT          388 a          764 c          781 g          299 t
ORIGIN      
        1 gtcggtgacg gcgtacacgg tgaagtagag accggagaac gctgcgagga agaccgccac
       61 ctggaagagc tggttgctga tcgggagcca gtccagcgac ggcagcggct cgggagcggt
      121 tccgacccag gccttgatca ccgtgtccgt gatggcgacc ttgccgaaca cgaggaagaa
      181 cgcgaacacc gagagcgaca gcagcagcac ctgcagcgcc tgggagaaca gcagcacgag
      241 gacgaggttc gcctgctgca gccgcgtcag gtcggcgtgc gcagcgtcgt tggtgagctc
      301 gtgggccgcg tcctcgaccg gagtccgcgc acagatcttc accagacggt caccttcggc
      361 ctcggcgctg acctgccgca cctcctccgg cagccggacg agcaggaaga ccaccgcgat
      421 cgccgcgaac agcatcaccg tcagccacag caggccacgg tcgagcgcac tgcagacctg
      481 ccagacctcg gtgttgatga agaggaacgt gatgaacaac agcagcagcg gcagggcgcg
      541 cgtcaccagc gggaacagcg agccgaggct cccgaacgtc tgccccgtcg cccagcggat
      601 gatcggcttg aggtgcaggg agaacaaccc gtaggcgagg acgaccagtc ccagcacgag
      661 cgcgccgacg acgatgccgc tggacacgtc cgacgacccg agcccggaga acaggccggc
      721 agcgacgccg agggcgagag ccaccgcgag gacagggatc agccgggacc gcgagagagc
      781 tgcatgcacc cgctccgcgt tgctcggcac gaagtacggc aacccttggt gctcgaacca
      841 gcgctcgacc gagcggatcg tcgctgcgcg atcctcctcc gtcacgcgat cacgctcacc
      901 gcaacgccgc aggtgcgggc tgcgtcgaca gcgtccgtac gccgttcggc gctccgggtc
      961 acgggcatgg ccgacaggct agccgtgacg gtgcggcgaa tcgctcaggc ggtggcgtcg
     1021 atgctgtccg gcgcggcggt ccaggaggcc ggggcgtcga cgcgtggcgt gtcgagcagc
     1081 atgcgggtgc gggcgacgct ggtctcgagc agctcgcaga gccggtcgtc ggaggccgga
     1141 gcgagcgcca ggctcatcac cgcgacccgg acgatgtgcg accgggcgcg ggcgaagtcg
     1201 ttgcctccgc tccacggccg ggcgacgaag tgctgcgccc aggtggtcgc ctgcgggcag
     1261 tgctccagcg cttcctcgat catcggcatc aaggccttgg tacgcgggtc gtccatggcg
     1321 tcgatctcgt cctcgcagcg aaggaggccg acggcgagtc ccttctgctg gtcgtaggcc
     1381 gcgaccggga aggccttggt ggccgccagc accgtgatgg tgacgtccac gaccgggtcc
     1441 tcgccgttca acccgaccac gtcggcgatc agcggcacga tccgctgccg tccggcatcg
     1501 gacaggttgt cgttgacgcc gcgcgccacg gtggccagca gtggatgcgt gcacgccggg
     1561 tggtcggaga acttctcccc cgccagccag gacgcgaact ccatgaagca cgcgccacga
     1621 cgcggattgc gatgcttgcc gcgcgagagc accggaacgc cttcgggcaa cgactgtcga
     1681 cgccgcattc gagatcaccc gacctgctgt ggagggagac tggttgttcc agtctgcgcc
     1741 ggaagtgggc gccgcaccac cccctggacc agcgagtttc cgggaatgga ccgcttcggc
     1801 atggtgtcga ccggcgtacg gcgaccgaca atcagccggc agggatgagc ggcggtgtcg
     1861 tcagcatggc ccgggcccgg tcgacgtcgt cggccatctg gaccagcagc gcgtccacgg
     1921 agtcgaactt gaccatccca cgcagccgct cctcgaaggc cacctccacg atcacgccgt
     1981 acagctcgag gtcgtcccgg tcgagcacgt aggactcgac ccgccggtcg gtgccgtcga
     2041 acgtcgggtt cgtcccgacc gagatcgccg ccgggaggta tgccgcatcc gggtcgtcga
     2101 gccggcgcag ccacccggcg tacacgccgt cggccggcac cgctgtacgg gacgactgca
     2161 ccgggacgtt cgcggtcggg taaccgagct cgcggccccg ctggtcaccc ttgacgacat
     2221 acccacggac cg
//
LOCUS       DATBFZ010000473         1745 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_2619285, whole genome shotgun sequence.
ACCESSION   DATBFZ010000473 DATBFZ010000000
VERSION     DATBFZ010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 1745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1745
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2619285"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..707
                     /locus_tag="VH419_14440"
     CDS             <1..707
                     /locus_tag="VH419_14440"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY7044863.1"
                     /translation="VDPVFARHARQLRDRLGAERVEVCIRFGSLADELTKIAGPTDVL
                     VTQAAPPGTAGLATTLGAHAEATVVAVRPTVARTRVAGPFSGHVVVGVDGAGRDSGPI
                     RFAFDYAARHGKPLTAVHAHEPESDGLWTDDQFMEIHPLGHTFGLDMLDAAVAAAHAD
                     YPRVAIRRAVMHQRAADALVHASAGAVLLVVGDRGRGPIARHLLGSVSRRVLSHGRCT
                     VAVVHGAPATPPAPAT"
     gene            complement(841..1383)
                     /locus_tag="VH419_14445"
     CDS             complement(841..1383)
                     /locus_tag="VH419_14445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044864.1"
                     /translation="MLTLVLLVAGGLLMWGYSFANSNVHDQLAAQQIYFPAQGSAALK
                     PAAIGPYLDQYAGQQLVTGAQAKAYADHFIAVHLSEVADGKTYAQVSALAQADPTNTT
                     LQGQVNTLFKGETLRGMLLNAYAFWKLGQLALYGAIVSFVLAAVMLALSALGIRHLRR
                     VPADAELLAPRARTAEVVNS"
BASE COUNT          278 a          594 c          593 g          280 t
ORIGIN      
        1 tggtcgaccc cgtattcgcg cgccacgcgc ggcaactgcg tgaccggctc ggtgccgagc
       61 gagtcgaggt ctgtatccgc ttcggttccc tcgccgacga gctgaccaag atcgctggcc
      121 cgaccgatgt gcttgtcacc caagcggcac cgcccggtac ggctgggctg gcgactaccc
      181 tcggcgcaca cgccgaggca acggtggtgg ccgtccgtcc gaccgtcgcc cgcaccagag
      241 tggccgggcc gttcagcggc cacgtggtcg tcggcgtcga cggtgccgga cgcgacagcg
      301 gcccgatccg gttcgcgttt gactacgccg cccgccacgg aaagcctttg acggcggtac
      361 atgcccacga acccgaaagc gacggtctat ggaccgacga ccagttcatg gagatccatc
      421 cgctcggtca cacattcggg ctcgacatgc tcgacgccgc ggtcgccgcc gctcacgccg
      481 actacccacg tgtagcgatc cggcgcgcgg taatgcacca gcgggcagcg gatgcgctgg
      541 tgcacgcgtc ggccggtgcg gtgctactcg ttgtgggcga ccgcggtcgc ggtccgatcg
      601 cccgccacct gctcgggtcg gtcagccgcc gcgtcctgtc ccacggccga tgcaccgtcg
      661 cagtcgtaca cggcgcaccg gcaaccccgc ccgcgccagc gacatagcgt ccgctctgag
      721 ccgtcggtcg gatccacgga tcgtcatcac gaggggccat cgcggttcga gatcgacgtc
      781 gtgagcaacg atgtgcgccc tgggtcggaa gcccagggcg cacgacgtgc ggtgcgatga
      841 ctagctgttg acgacctctg cagtgcgagc tcgaggtgcc aagagttccg cgtccgcggg
      901 gacccggcgg aggtgccgga tgccgagagc ggacagggcc agcatcaccg cggccagtac
      961 gaagctgacg atcgcaccgt agagcgcgag ttggccgagc ttccagaacg cgtacgcgtt
     1021 gagcagcatg ccgcgcagcg tctcaccctt gaacagcgtg ttgacctgcc cctgcagggt
     1081 ggtgttcgtc gggtccgcct gcgccagtgc gctgacctgc gcataggtct tgccgtcggc
     1141 gacctcggac aggtgcaccg cgatgaagtg gtcggcgtac gccttggcct gcgcaccggt
     1201 gacgagttgc tgaccggcgt actggtccag gtacgggccg atcgccgccg gcttgagggc
     1261 ggcgctgccc tgggccggga agtagatctg ctgggcggcc agctggtcgt ggacgttgct
     1321 gttcgcgaag ctgtagcccc acatgagcag cccaccggca accagcaaga ccagggtcaa
     1381 catgacaccg accgaggaga ccagggtgtc aaaagtcctg cgacgcattg tggttcgcct
     1441 cttctcaacg acactgttcg gcccggtcgg cctggccagt gattgcctgc gaactcatgg
     1501 tcgcgaccac gcaccccgcc cggacagagg ccaaggtccc ccgcacccgg gacaaaggac
     1561 agtcatttag ggagagccga acggccgtcg ggtgtggacc tacgagccag ccgttctcgc
     1621 ccccgcatgc tcggatgggc ggggtgcacg ccagaccggt cacgccgtgt tggcgtcgag
     1681 tgcgagcgag cggactagat gggtgtcatc tggtatgtct cgacctgcat ccacggtccg
     1741 tctcc
//
LOCUS       DATBFZ010000474         1813 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_3608090, whole genome shotgun sequence.
ACCESSION   DATBFZ010000474 DATBFZ010000000
VERSION     DATBFZ010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3608090"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            36..860
                     /locus_tag="VH419_14450"
     CDS             36..860
                     /locus_tag="VH419_14450"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY7044865.1"
                     /translation="MASPNRKEQPFIAAERTAAIEAFRLRYGSDLTFAPILVVIAAFD
                     EEDSLGEVLTHIPPDVTGLPVDTLVVDDGSHDATCEVAEKYNRVRVVRLARNCGHGVA
                     LRLGYRLAAEHGARYVVTLDADMQWDPREMPIVLPPLLDGEADFVIGSRVLGVAETDD
                     SFRHAGVRFFASLVRVLTGVAVTDTSSGYRAMRTEVTQRVPQTQVQYQTSELLIGAIY
                     AGYRITERPITMHKRFAGESKKGHNVLYGLRYARVIVQTWWRERARSRREGRSTAR"
     gene            971..>1813
                     /locus_tag="VH419_14455"
     CDS             971..>1813
                     /locus_tag="VH419_14455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016669928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY7044866.1"
                     /translation="MKVLVLGGDGFCGWPTSLHLSAQGYDVKIVDNFARRVADVELET
                     SSLTPISPLSTRLATWTTLTGTKIGFERFDVADHYHRLLTLIAEWRPDAIVHFAEQRA
                     APYSMKSSWHKRYTVSNNLNATNNLLAALVEAECDAHVVHLGTMGVYGYGTAGMKIPE
                     GYMRIQVPTDDGRVVESEILYPPNPGSIYHMTKTQDQLLFAYYNKNDGVRVTDLHQGI
                     VWGTQTRETALDQRLINRFDYDGDYGTVLNRFLMQAALDYPLTVHGSGGQTRAFIHIQ
                     DTVRC"
BASE COUNT          300 a          633 c          597 g          283 t
ORIGIN      
        1 tggcgtgtcc ccggggcccg agtagcctcc ccgccgtggc ttccccgaac cgcaaggagc
       61 agcccttcat cgcggcggag cggactgcgg cgatcgaggc cttccgcctc cgctacggct
      121 ccgacctgac cttcgcgccg atcctggtcg tcatcgccgc cttcgacgag gaggacagcc
      181 tcggggaggt gctcacgcac atcccgcccg atgtgaccgg gctcccggtc gacaccctcg
      241 tcgtcgacga cggcagccat gacgcgacgt gcgaggtcgc ggagaagtac aaccgggtgc
      301 gggtcgtgcg cctggcgcgc aactgcgggc acggtgtcgc gctgcgcctg ggctaccggc
      361 tggccgccga gcacggtgcg cgctacgtcg tgaccctgga tgccgacatg cagtgggatc
      421 cgcgggagat gccgatcgtg ctgcctccgc tgttggacgg cgaggcggac ttcgtcatcg
      481 gctcccgggt cctcggcgtc gccgagaccg acgactcctt ccgccatgcc ggtgtgcggt
      541 tcttcgcgtc cctggttcgc gtcctcacgg gggtcgccgt caccgacacg tccagcggat
      601 accgcgcgat gcgcaccgag gtgacccaga gggttccgca gacgcaggtg cagtaccaga
      661 cctccgagct cctgatcggc gcgatctacg ccggctaccg catcacggaa cgaccgatca
      721 cgatgcacaa gcggttcgcg ggcgagagca agaagggcca caacgtcctg tacgggctgc
      781 gctacgcccg ggtgatcgtg cagacctggt ggcgggagcg cgcccgctcg cgccgggagg
      841 ggcgcagcac ggcacggtga cagccgacga ggacaccagg cacgccggtg ccaccgacct
      901 ggtcccagcc tggacggcgc ctgtggttcg cccggatacc ctgcttggcg gacccgacga
      961 aaggcgtggc atgaaggttc tcgttctcgg tggggacggc ttctgcgggt ggccgacctc
     1021 gttgcacctg tcagcccagg ggtacgacgt gaagatcgtc gacaacttcg cgcgtcgtgt
     1081 cgccgacgtc gagctggaga cgtcctcgct caccccgatc tcgccactct cgacgcgtct
     1141 ggccacgtgg acgacgctga ccggcacgaa gatcgggttc gagcgtttcg acgtggccga
     1201 tcactaccac cgcctgctca cgctcatcgc ggagtggcgg ccggacgcga tcgtccactt
     1261 cgccgagcag cgggcggcgc cgtactccat gaagtcgtcg tggcacaagc gctacacggt
     1321 ctccaacaac ctcaacgcga ccaacaacct cctcgcagcc ctggtcgagg cggagtgcga
     1381 cgcccacgtc gtgcacctcg ggacgatggg tgtgtatggc tacggcaccg ccgggatgaa
     1441 gatcccggag gggtacatgc gcatccaggt tccgacggat gacggtcggg tcgtggagtc
     1501 ggagatcctc taccccccga acccgggctc gatctaccac atgaccaaga cccaggacca
     1561 gctgctcttc gcctactaca acaagaacga cggcgtccgg gtcaccgacc tccaccaagg
     1621 catcgtgtgg ggcacccaga cgcgcgagac cgctctggac cagcggctca tcaaccgctt
     1681 cgactacgac ggtgactacg gcacggtgct caaccgattc ctgatgcagg cagcgctgga
     1741 ctatcccctg accgtccacg gcagtggtgg gcagacgcga gccttcatcc acatccagga
     1801 cacggtgcgt tgc
//
LOCUS       DATBFZ010000475         5241 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_6077329, whole genome shotgun sequence.
ACCESSION   DATBFZ010000475 DATBFZ010000000
VERSION     DATBFZ010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 5241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5241
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6077329"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..332)
                     /locus_tag="VH419_14460"
     CDS             complement(60..332)
                     /locus_tag="VH419_14460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015069232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEY7044867.1"
                     /translation="MASITVKVGSSVGLHARPATIISEKAGELGVEVTLATPDGEPVE
                     ADSALLIMTLGASQGDEVIVASDDESAARTIADLVELDLDDPNYPA"
     gene            complement(382..2460)
                     /locus_tag="VH419_14465"
     CDS             complement(382..2460)
                     /locus_tag="VH419_14465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015069233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HEY7044868.1"
                     /translation="MSELINSALVALDASYGDTKDDVIHALAGLVAADGRSTDAAGLA
                     DDAFAREAKSVTGMPGGLAIPHCRSEYVLEPTLAFARLSPPVDFGAKDGPADLVFMIA
                     APAKGDQEHMKLLTKLARALVRKEFTDALRSATSAQQVVELVTDVVAPAPVAVTSSAA
                     EPAATTQPAPAPRTEHSAAPGAGTTIVAVTSCPTGIAHTYMAAEALAEAGAKAGVNLV
                     VEPQGSVGAEKVAPSVIRSASAVIFAADVGVRDKGRFAGLPEVKASVKQGIHEADDLV
                     ARALAAADDPNAPRVSGDGAVADEGSGEQSVWIKARTALLTGVSYMIPFVAAGGLLIA
                     LGFAFGGYQVDPTLATTNSFTHLPDASQAGGALFNSPFFLYLGAVMLFIGKAAFALFI
                     PALGGYIAYAIADRPGIAPGFVVGLISGGGGAVGGLDGVGAGFIGAIVGGFVAGVVAL
                     WISRWPVPRFVRGLMPVVIIPLLATIIAAGLMVVFLGRPLSWLTSELTDGLSSMSGTY
                     SVLLGVVLGLMMCFDLGGPVNKAAYVFATTGLGAAASSANLRIMAAVMLAGMVPPLAM
                     ALASTVRPQLFTEPELENGRAAWLLGASFISEGAIPFAAADPLRVIPAMMLGGAITGG
                     LSEAFAVELSAPHGGIFVLFAVDGVFWFLVALAAGVIAAAATVIALKTAHRSTKEVRA
                     DAEAPVPATV"
     gene            complement(2487..3434)
                     /locus_tag="VH419_14470"
     CDS             complement(2487..3434)
                     /locus_tag="VH419_14470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007078324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HEY7044869.1"
                     /translation="MIVTLTANPSVDRTMAIERPIRAGEVFRALSVGDEAGGKGVNVA
                     RAVAHAGVPAVAVLPADDDDLLVKGLVDAGVHPINVAVGRPSRVNITVTEPDGTTTKF
                     NDPGHTFVDGVFTQLEASLVARSQGDGWVVLCGSLPPSAPVGWYAALTSRLRSAGRRV
                     AVDTSGAALTAAVTGNHRPNLVKPNAEELASLTGDDPDRLEGDVTASTAAAQQLVDDG
                     IDAVLVTLGARGAVLVRSDGVWHASPPPITLRSTVGAGDASLAGYLLADLRGLGPAQR
                     LCEAVAYGAAAASLPGSTMPRESDLDRDGVVVTTLIAAR"
     gene            complement(3431..4210)
                     /locus_tag="VH419_14475"
     CDS             complement(3431..4210)
                     /locus_tag="VH419_14475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007078323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HEY7044870.1"
                     /translation="MYAEERQQAIADLVNRDRRVSVSHAAETFGVTTETVRRDLAMLE
                     RHGLVHRVHGGAVAAGSLTTVEPAMTERQEAAATQKERIAQAALDLLPADRGSILLDA
                     GTTTQRLAASLPTDRAYKVITNAATIATAVAANATTSGRNELHLLGGRIRATTLASVG
                     PQTLAALHDIRVDVAFVGTNGLTLQHGLTTPDVDEAAVKSAMVAAGRRVVVLADSRKF
                     GQETLVRFARLDQIDAVVTDDGVSPTDVAALESLDIEVVIA"
     gene            4393..>5241
                     /locus_tag="VH419_14480"
     CDS             4393..>5241
                     /locus_tag="VH419_14480"
                     /inference="COORDINATES: protein motif:HMM:NF017346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-utilizing N-terminal
                     domain-containing protein"
                     /protein_id="HEY7044871.1"
                     /translation="MVETRSVTEAAATRTRSGTPVVPGIAVGPVIRPAAAVDLGGFAA
                     AAAGTPEDEAGRFDAAVAQVAARLQRRADDAVGVAKEVLTAQVGLVADKGLRKAVDKS
                     IAAGSSAEAATAAAVEQFTVMFEKVGGMMAERITDLRDLGSRVVAELQGLPEPGIPRP
                     ETPSVLMAADLAPADTAGLDPERVIALVTSLGGPTSHTAIIARQLSIPCVVAADVDDV
                     TPGTVVLVDGAAGDIVIEPDVEEAAALVEQDRRTRAAADAWTGPGRTADGLEVAIYAN
                     VQDGASA"
BASE COUNT          823 a         1787 c         1857 g          774 t
ORIGIN      
        1 aaacatcgca aacccctcgg ggcggccgcg cacgtgaggt gcgaaaggcc ggggcgggtt
       61 taggcggggt agttggggtc gtcgagatcg agctcgacga gatcggcgat cgtgcgcgcc
      121 gccgattcgt cgtcgctggc gacgatcacc tcgtcaccct ggctggcccc gagggtcatg
      181 atgagcagcg ccgagtccgc ctcgacgggc tcgccgtccg gtgtcgccag cgtgacctcg
      241 acaccgagct cgccggcctt ctcggagatg atcgtggccg gtcgggcgtg cagcccgacc
      301 gacgagccga ccttgacggt gatgctggcc atcagtgcgt cctctcgttg gtatgacgtg
      361 aatttggaca ggtcggccgg atcagaccgt cgccggtacc ggcgcctcgg catcggcccg
      421 gacttccttc gtcgagcgat gcgcggtctt caacgcgatc accgtggccg cagctgcgat
      481 gacgccggct gccagtgcga ccaggaacca gaacaccccg tcgacggcga acaggacgaa
      541 aatgccaccg tgcggcgcgc tcagctcgac cgcgaaggcc tcggacaagc cgcccgtgat
      601 cgcaccgccc agcatcatcg cggggatcac ccgcaacggg tcggcagccg cgaacgggat
      661 cgcgccctcg gagatgaacg atgcgccgag caaccacgcg gcacgaccgt tctcgagctc
      721 cggctcggtg aacagctgcg gacgcaccgt cgaggcgagc gccatcgcca gcggcggcac
      781 catgccggcg agcatgaccg cggccatgat ccgcaggttc gcggaactgg cggcagcacc
      841 caggccggtc gtcgcgaaga cgtacgccgc cttgttcacc ggaccaccca ggtcgaagca
      901 catcatcagg ccgagcacga cgcccagcag gacggagtac gtaccgctca tgctggacag
      961 gccgtccgtc agctcgctcg tcagccacga cagtggtcga ccgaggaaca ccaccatcag
     1021 gccggcagcg atgatggtcg cgagcagcgg gatgatcacc accggcatca ggccgcggac
     1081 gaacctgggg acgggccacc gggagatcca cagggcaacg acaccggcca cgaacccgcc
     1141 gacgatggca ccgatgaacc ccgcgcccac accgtccagg ccgccgactg cgccaccacc
     1201 accgctgatc agcccgacca cgaagccggg tgcgattcct ggccggtcgg cgatcgcgta
     1261 cgcgatgtag ccgcccagcg ccgggatgaa cagcgcgaac gccgccttgc cgatgaacaa
     1321 catgaccgcg ccgagataca ggaagaacgg gctgttgaac agcgcaccgc ccgcttgcga
     1381 cgcgtccggc agatgcgtga acgagttcgt ggtcgcgagc gtcgggtcga cctggtagcc
     1441 accgaaggcg aacccgagcg cgatgagcag accaccagcg gcgacgaacg ggatcatgta
     1501 gctgacgccg gtgagaaggg ccgtacgggc cttgatccac accgactgct cgccggaacc
     1561 ctcgtcggcg acggccccgt cgcccgacac ccgcggggcg ttcggatcgt cggccgcggc
     1621 cagcgcccgc gcgacgaggt cgtcggcctc gtgaatgccc tgcttcacac ttgccttgac
     1681 ctcgggaagc ccggcgaacc gccccttgtc ccgtacgccg acgtcggcgg cgaagatgac
     1741 ggcgcttgcg gagcggatga cgctcggggc gaccttctcc gcgcccaccg aaccctgcgg
     1801 ctcgacgacc aggttgacgc cggccttcgc accggcctcg gcgagcgcct ccgcggccat
     1861 gtaggtgtgc gcgatgccgg tggggcacga ggtcaccgcc acgatggtcg tgccggcacc
     1921 gggggccgct gagtgctccg tcctcggcgc aggggccggc tgcgtcgtgg ctgcgggctc
     1981 cgcggccgat gacgtgaccg cgaccggcgc tggcgcgacg acatccgtca cgagctcgac
     2041 cacctgctgc gctgaggtcg ccgaccgaag ggcgtcggtg aactccttgc gtaccagtgc
     2101 ccgagcaagc ttggtgagca gcttcatgtg ctcctggtcg cccttcgccg gggcggcgat
     2161 catgaacacc agatcggccg ggccgtcctt ggcgccgaag tcgacggggg gcgacaggcg
     2221 ggcgaaggcg agcgtgggct cgaggacgta ctccgaccgg cagtgcggga tggcgagtcc
     2281 gccgggcatg ccggtcacgg acttcgcctc gcgggcgaac gcgtcgtccg cgagtccggc
     2341 tgcgtcggta cttcgtccgt ccgctgcgac cagtccggcc agcgcgtgga tgacgtcgtc
     2401 cttcgtatcg ccgtaggagg cgtccagcgc cacgagtgcg gagttgatga gttcagacac
     2461 ggtgctttcc ttgaggtgaa tgcggctcag cgagcagcga tgagcgtcgt gacgacgaca
     2521 ccgtcacgat cgaggtcgga ctcccggggc atcgttgacc cgggcaggga ggcagcggcg
     2581 gccccgtatg cgaccgcttc gcaaagccgt tgggcaggac cgagtcctcg caggtcggcc
     2641 aacagatagc cagccaggct ggcgtcgcct gcgccgacgg tgctgcgcag ggtgatcggg
     2701 ggagggcttg catgccagac gccatcgcta cgcacgagga cggcacctcg ggcaccgagc
     2761 gtgaccaaca cggcgtcgat gccgtcgtcg accagctgtt gtgccgccgc cgtcgaggca
     2821 gtgacgtcgc cctcgagacg gtccgggtcg tcgccggtga gggaggccag ctcctcggcg
     2881 ttgggcttga cgaggttggg gcggtgattg cctgtcacgg ccgctgtcag cgctgccccc
     2941 gacgtgtcga ccgcgacgcg gcgtcctgcg gaccgcagcc gagacgtcag cgcggcgtac
     3001 cagccgaccg gggctgacgg cggcagtgag ccgcacagca cgacccagcc gtcgccttgc
     3061 gagcgcgcca ccagcgacgc ctcgagctgg gtgaagaccc cgtcgacgaa cgtgtggcca
     3121 gggtcgttga acttcgtcgt cgtgccgtcc ggctcggtga cggtgatgtt gacccgtgac
     3181 gggcggccga cggccacgtt gatcgggtgg actccggcgt cgacgagccc tttgaccaac
     3241 aggtcgtcgt cgtccgcggg gaggacggcc acggccggta cgccggcatg agcgaccgcg
     3301 cgggcgacgt tgacgccctt gccaccggcc tcgtccccga cgctcagcgc gcggaagacc
     3361 tcgcccgccc tgatcgggcg ctcgatggcc atggttcggt cgacggaagg attcgccgtg
     3421 agtgtcacga tcatgcgatc accacctcga tgtcgagcga ctcgagggcc gcgacgtcag
     3481 tcggcgagac gccgtcgtcg gtgaccacgg cgtcgatctg gtcgagccgg gcgaagcgga
     3541 ccagcgtctc ctgaccgaac ttccgggagt cggcaagaac cacgacacgg cggccggccg
     3601 ccaccatcgc cgacttcacc gccgcctcgt cgacgtccgg cgttgtgagg ccgtgctgaa
     3661 gcgtcaggcc gttggtgccg acgaacgcca cgtcgacacg gatgtcgtgc agcgcggcca
     3721 gcgtctgtgg cccgacgctt gccagcgttg tggcgcgaat tcggccgcca agcaggtgca
     3781 gctcgttgcg tcctgatgtg gtggcgttgg cggcgacagc ggtcgcgatc gtggcggcgt
     3841 tggtgatcac cttgtacgcg cggtcggtcg gcaagctggc ggccagccgt tgggtcgtcg
     3901 taccggcatc cagcaggatg ctgccgcggt cggcggggag caggtcgagc gcagcctgcg
     3961 cgatccgctc cttctgcgtt gccgctgcct cctgacgctc ggtcatggcc ggctcgacgg
     4021 tggtcagcga gccggccgcc acggcgccgc cgtgcacgcg atggaccagc ccgtggcgtt
     4081 cgagcatcgc caggtcgcgg cggaccgtct cggtggtgac gccgaacgtc tccgccgcgt
     4141 ggctcacgct gacccgacgg tcgcggttca ccaggtcggc gatcgcctgt tggcgttcct
     4201 ctgcgtacaa cggttgtcct tcgcgtggaa gcggctgagg tgatgttgct tcgtattgtt
     4261 ttacgcccga acagttgcga tgtcaagtcc tcatgcggta aagttccagt agatcccaaa
     4321 gaaaaccaca acagcatctg ctgcacaccg acacgatgct tcgcgcaccg cggttccaag
     4381 gaggtccccc cgatggtcga gacgagatct gtaaccgagg ccgctgccac ccgcacgcgc
     4441 tccgggacac cggtcgtgcc gggcatcgct gtgggccctg tcatcaggcc ggcggcggca
     4501 gttgacctcg gcgggttcgc cgcggcggct gcggggactc cggaggacga agccggtcgc
     4561 ttcgacgcgg ccgtcgcgca ggttgctgca cggctgcaac ggcgcgccga cgatgcggtc
     4621 ggtgtcgcca aggaggtgct caccgcccag gtgggtctcg tcgctgacaa gggcctgcgg
     4681 aaggccgtcg acaagtcgat cgctgccgga tcgtccgcgg aagcagccac ggcagctgcg
     4741 gtcgagcagt tcaccgtgat gttcgagaag gtcggcggca tgatggccga gcgcatcacc
     4801 gacctgcgtg acctgggttc ccgggtggtc gccgagctcc agggactacc ggagcccggt
     4861 attcctcggc cggagactcc gtcggtcctg atggccgccg atctcgcgcc cgctgacacg
     4921 gctggactcg acccggagcg ggtcatcgcg ttggtgacga gcctgggtgg gcccacgagt
     4981 cacacggcga tcatcgcccg acagctctcg atcccgtgtg tggtggccgc cgacgtcgac
     5041 gacgtgacgc ccgggaccgt cgtgctcgtc gacggggcgg ccggcgacat cgtcatcgag
     5101 cccgacgtcg aagaggcggc cgcactggtc gagcaggacc ggcgtacccg cgccgccgcc
     5161 gatgcgtgga ccgggcccgg ccgaaccgcc gacggtctcg aggtcgcgat ctacgcgaac
     5221 gtgcaggacg gtgcctcggc g
//
LOCUS       DATBFZ010000476         7270 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_5418817, whole genome shotgun sequence.
ACCESSION   DATBFZ010000476 DATBFZ010000000
VERSION     DATBFZ010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 7270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7270
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5418817"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1342)
                     /locus_tag="VH419_14485"
     CDS             complement(<1..1342)
                     /locus_tag="VH419_14485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEY7044872.1"
                     /translation="MSAALPPLSVRAPSSEASAAAQRIRFRRALVLTGMTVVAPGSAQ
                     LVAGNKRVGRIALRCAAAGLVFVVGLVVLGLVHRSELISAFTRPGFLLLLRIGLIAYA
                     VGWAYLVVDAWRISEPMSLGRGHRLFVTAMNGLLCFGLSGVLLFGSHLIAVQRDFITS
                     VFGGDEVTSATHGRYNILLLGGDAGRGRVGLRPDSMTVASIDEETGRTVLIGLPRNLA
                     DVPFPEGSAMAEEFPHGFDCDGCYLNAVNTWANDHPELFPHDESPGITATTQAIEQIT
                     GLSINYYALIDLRGFRDLVDAVGGVDIKVGERTPIGGVAAPISGWIEPGRQHLDGFQT
                     LWYARSRATTNDYSRMARQKCVMNAMLHQLNPTKVLTNFGAIAEAGKEVVSTSIPASQ
                     LDTFVNLSLKARSLPVSTVSFVPPAIDTSHPDFAKIRSMVTRAIDASQAKDDGSH"
     gene            1454..2773
                     /locus_tag="VH419_14490"
     CDS             1454..2773
                     /locus_tag="VH419_14490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEY7044873.1"
                     /translation="MPDDRTEEEHYDWLYAGHAGRDGPRQDGDATDKARRSAAGTVSP
                     DTRPIEPVRSDERDEPERTQVLGVGPEAPPSTPGTDKPTTFGGTYAAPTRRSDEKPRF
                     ATPASPQQAPSRPTEPPSSTRPQPPGKRPRRRRKWWVIGIVSVLVAWILFLVLVPVWA
                     WSKISKVDAEPSGDRPADTPGTTYLLVGSDARKGVGGARTDTILLLHIPDGGGPDLLL
                     SIPRDSYVDIPDHGKNKINAAFAFGGPKLLVQTVEDNTGIRVDDYIEIGFTGFVDVVD
                     AVGGIEVCPKTAVNDSKAGHLKLHKGCQEVDGQTALGYSRSRAFPLGDITRTEHQREV
                     ISKVGKEAASWSTILLPWRYFSVNKAAAESLRIGENVGPLDLVQFAWAMAHSGDAKRC
                     VVPFSSLGTTTNAGSSVIWDEAKAKALFAKIRADDTAGAVCNSGQRR"
     gene            2861..3394
                     /locus_tag="VH419_14495"
     CDS             2861..3394
                     /locus_tag="VH419_14495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020388808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HEY7044874.1"
                     /translation="MTTSPAPAETSTRAPWHDAGRRALPWAGLVVRLVVGGVWIAAGL
                     LKLPDPAESVRAVRAYRLLPEFTVHAVGYGLPFLEIIVGLLLVVGLGTRIAAVISAVL
                     FVLFIVGITAAWARGLQIDCGCFGGGGYAADATSKYPWEIARDAGLLLGSVLLVIWPR
                     TRLSLDGWLVPDARSEA"
     gene            3391..4146
                     /locus_tag="VH419_14500"
     CDS             3391..4146
                     /locus_tag="VH419_14500"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEY7044875.1"
                     /translation="MSKKGRQASERIKAMQAQQAAAERRRRALVIGVIVAVVIAAVVG
                     IGVAVQSNRNETTSGAAPAGVTADNGILRGKDSAPVSVVVYEDFQCPVCKAFEENVGA
                     MLTDKIDQGVVQLEYRPIAFLDRASTTKYSSRALATAACALEDGGPDVFHKLHDLLFA
                     NQPEEGSDGLPDSKLADLAAEAGADKASVESCQEAGTFDGWVAKVTDQASKDGISGTP
                     TYFVDGDKVTFTNGEDPKVTLGNLIDQAASSDQ"
     gene            complement(4159..5655)
                     /locus_tag="VH419_14505"
     CDS             complement(4159..5655)
                     /locus_tag="VH419_14505"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY7044876.1"
                     /translation="MSDPDAETAAPSPDRPVVLRGGTVVTMDDSHTVLTDADVLVVDD
                     TIAAVGRGLDVPDGTQEIDASDGIVMPGMIDTHRHMWQTAMRGYGADWTLTQYFVWYY
                     LEHGKAFRPEDVYAGNLVSAWDALEAGVTTTVDWSHGLQTVDHAEAAVDALTSVPGRF
                     VLAYGNIQAGPWEWTADPAVQGFLERHRSGAGDRLGFQLAFDVTGDPAFPEKAAFEVA
                     RELGLPVTTHAGVWGATNDDGIRLMHENGFMTPENVYVHAATLTTDSYHRIAATGGSV
                     SVSTESEQSAGQGYPPTWQVRKHDIPVSMSMDTSVWWSSDLFSAMRTTLGADRSREHL
                     EAHAKGDTVTHAHLRAEHVVEWATRGGAHALGRDDLGRLEAGKKADVVLIKNDTSPVS
                     FPLLNPYGHIAFQAQRGDVHTVLVDGKVVKHAHRLVGVDLPAVRRTVEATVDHLRTTL
                     GDEAWTGGMNPDLPHEEVLDNPYQYTDYKSDSTRGARGTVFGEPTASG"
     gene            5851..6678
                     /locus_tag="VH419_14510"
     CDS             5851..6678
                     /locus_tag="VH419_14510"
                     /EC_number="4.1.3.34"
                     /inference="COORDINATES: protein motif:HMM:NF004868.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citryl-CoA lyase"
                     /protein_id="HEY7044877.1"
                     /translation="MTTSSDGLSFPTSLGTSTPDRIQLLGQDLTADLMGNVGFGELAF
                     WLVTLQRPTPSQTRVFEAVLVALADHGFTPTAIAARLTYLSAPDSLQGALAAGLLGGG
                     SRFLGVTEDCGAYLHTVLAAHEGTLPDDDDGWDALALAAVRAQHASGRYVPGLGHPVH
                     KVEDPRTAALIRIADEEGLRGPHLRLFEAIGRVHEQVLGRSLPLNGAGVCGAALADLG
                     LPVELLRGFALLARSAGLLGQLAEERRRPIGMDAYLAVDRNAVYVDPADGDGRDEAD"
     gene            6706..>7270
                     /locus_tag="VH419_14515"
     CDS             6706..>7270
                     /locus_tag="VH419_14515"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044878.1"
                     /translation="MATVAAVIASTHHPFYYRASTSTGEDRPPFADEWVSKIEAFRET
                     LTRAEPDVLVMVGSDHFHQLWLDNMPQFLVGKAPFFDANFYNEEREFGLPRMTLAGDE
                     ELAAYILRNGLDADFDLAFSNELRIDHSITCPIITLRPEADLPIVPIYTNIFAPPLPQ
                     PKRFVQLGAAIREIVESWPSDLRVAIIG"
BASE COUNT         1175 a         2511 c         2453 g         1131 t
ORIGIN      
        1 tgtgactccc gtcgtccttg gcctgcgagg cgtcgatggc gcgcgtgacc atgctgcgga
       61 tcttcgcgaa gtcggggtgg ctggtgtcga tggcgggggg tacgaacgag accgtcgaca
      121 ccggcaggct ccgcgccttc aacgacaggt tcacgaacgt gtcgagctgg ctcgccggga
      181 tggacgtgga cacgacctcc ttgcccgcct cggcgatcgc gccgaagttg gtgaggacct
      241 tggtcgggtt gagctggtgc agcatcgcgt tcatcacaca cttctggcgc gccatccgcg
      301 agtagtcgtt cgtcgtcgcg cgactgcggg cgtaccacag cgtctggaaa ccgtccaggt
      361 gctgacggcc gggttcgatc catccggaga tcggagcggc tacgcctccg atcggcgttc
      421 gctcccccac cttgatgtcg acgccgccca ccgcatcgac caggtcccgg aagcccctga
      481 ggtcgatgag ggcgtagtag ttgatgctca agccggtgat ctgctcgatc gcttgcgtcg
      541 tcgcggtgat gccgggactc tcgtcgtgcg ggaacagctc ggggtggtcg ttcgcccacg
      601 tgttgaccgc gttgagatag cagccatcac agtcgaaacc atgcgggaac tcttcggcca
      661 ttgccgagcc ttcgggaaac ggcacgtcgg cgaggttacg aggaagcccg atgaggaccg
      721 tccggccggt ctcctcgtcg atacttgcga ccgtcatgct gtccggccgc aacccgaccc
      781 gacctcgtcc tgcgtcgcca cccagcagca agatgttgta acggccgtgc gtcgccgacg
      841 tcacctcgtc cccgccgaag accgacgtga tgaagtcgcg ttggacagcg atgaggtggc
      901 tgccgaacag cagcaccccc gacaacccga aacacagcag tccgttcatc gcggtgacga
      961 acaagcgatg cccacgcccg agcgacatcg gctcggagat ccgccacgca tcgacgacca
     1021 ggtaggccca cccgacggcg tacgcgatga ggccgatccg cagcaggagc aggaaccctg
     1081 ggcgggtgaa ggccgagatc agctcgctgc ggtgaacaag cccgagcacg accagcccta
     1141 cgacgaacac caggcccgcc gcggcgcagc gcagcgcgat ccggccgacc cgcttgttgc
     1201 cggccaccag ctgggcactc cccggcgcca cgacggtcat gccggtcagc accagggcac
     1261 gccggaagcg tatccgctgg gcggccgccg aggcctcgct cgacggcgcg cgtacagaca
     1321 gcggcgggag cgcggcagac atgccgatca gtatcaccag tcactctggt cacagccgtc
     1381 acgacccgcc gacgaacagc agaccgctgc tgccagacgg aggccatcat catcggcagg
     1441 tagccttctc cgcatgcctg acgaccggac cgaggaggag cattacgact ggctgtacgc
     1501 cggtcatgcc ggtcgggacg gtccacgcca ggacggcgac gccactgaca aggcccgccg
     1561 gtccgctgcg ggcaccgtgt cgcccgacac tcggcccatc gaacccgtgc ggagcgacga
     1621 acgcgacgag cccgagcgca cccaggtgct cggtgtgggc ccagaggctc caccgagcac
     1681 cccgggcacc gacaagccga cgacgttcgg tgggacgtac gccgctccaa cgcgccggtc
     1741 ggacgagaag ccacgattcg ccaccccggc gagccctcag caggcgccgt cgcgacccac
     1801 cgaaccaccg tcgtcgaccc gcccgcaacc gcccggcaaa cgcccgcgac ggcgacggaa
     1861 gtggtgggtc atcggcatcg tgtccgttct cgtggcctgg atcctcttcc tcgtgctcgt
     1921 tcccgtctgg gcgtggtcga agatcagcaa ggtcgacgcc gagccgagcg gtgaccggcc
     1981 ggcagacacg cctgggacga cgtacctgct cgtcggttcc gacgcccgga agggggtcgg
     2041 cggtgcgcgc accgacacga tcttgctgtt gcacatcccc gacggcggcg gccccgactt
     2101 gctgttgtcc atcccgcgag actcctacgt cgacatccct gaccacggga agaacaagat
     2161 caacgcggcg ttcgcattcg gcggccccaa gctgctcgtg cagaccgtcg aagacaacac
     2221 cggaatccgg gtggacgact acatcgagat cgggttcacc gggttcgtcg acgtcgtgga
     2281 cgccgttggc gggatcgagg tctgtccgaa gaccgcagtc aacgactcga aggccgggca
     2341 cctgaagctg cacaagggtt gccaggaggt cgacggacag accgcgctcg gctactcgcg
     2401 gtcgagggct ttccccctcg gcgacatcac gcgcaccgag caccaacgtg aggtgatcag
     2461 caaggtgggc aaggaggcgg cctcgtggtc gacgatcctc ctgccctggc gctacttcag
     2521 cgtcaacaag gccgccgcgg agtcactgcg tatcggcgag aacgtcgggc ccctcgacct
     2581 ggtgcagttc gcctgggcga tggcacacag cggtgacgcc aagcgctgtg tcgtgccgtt
     2641 cagcagtctc ggcacgacca cgaacgccgg atcgtcggtg atctgggacg aggcgaaggc
     2701 caaggccttg ttcgcgaaga tccgcgcgga cgacacggcg ggtgccgtct gcaacagtgg
     2761 tcagcgacgc tgagcgtgag cgacgtcacc gactgtcacc gattttgacc gggcatgcgg
     2821 gaacgtctcc ggacatcacc tcgttaggct gctcgaaacc atgacgacct cgccggcacc
     2881 cgccgagacc tccacccgag cgccctggca cgatgccggg cgacgcgcgt tgccgtgggc
     2941 gggtctcgtg gtgcggctcg tcgtcggcgg cgtctggatc gccgccggcc tgctcaagct
     3001 gcctgacccc gcagagagcg tccgcgcggt ccgtgcgtac cggctgctcc cggagttcac
     3061 cgtgcacgcg gtcggctatg gactgccgtt cctcgagatc atcgtcggcc tgctgctggt
     3121 ggtcggcctc ggcacccgga tcgccgcagt catctccgcc gtcttgttcg tgctcttcat
     3181 cgtcggcatc accgctgcct gggcgcgtgg cctgcagatc gactgcggtt gcttcggtgg
     3241 cggcgggtac gccgccgacg ccacgagcaa gtatccgtgg gagatcgccc gtgacgcggg
     3301 actgctcctc ggatcagtac tgctcgtgat ctggccgcgc accaggctga gcctcgacgg
     3361 ttggctggtc ccggacgcga ggagtgaggc atgagcaaga agggtcggca ggcatccgag
     3421 cggatcaagg cgatgcaggc gcagcaggcg gcggccgaac gacgtcgccg agcgctcgtc
     3481 atcggagtca tcgtggcggt tgtcatcgct gcggtcgtcg gcatcggagt ggccgttcag
     3541 tcgaatcgga acgagacgac cagcggagca gcaccggccg gtgtcaccgc ggacaacggg
     3601 atcctgcgcg gcaaggactc ggcgccggtg tcggtcgtcg tgtacgagga cttccagtgc
     3661 ccggtgtgca aggcgttcga ggagaatgtc ggcgcgatgc tcaccgacaa gatcgaccaa
     3721 ggcgtcgtcc agctcgagta tcgcccgatc gcgttcctcg accgcgcctc gaccacgaag
     3781 tactcgtccc gcgccctggc gacggcagcc tgcgcgctcg aggacggcgg gccggacgtg
     3841 ttccacaagc tgcacgacct gctcttcgcc aaccagccgg aggaggggtc ggacgggcta
     3901 ccggactcga agctggccga cctcgcggcc gaggcgggtg ccgacaaggc gtcggtggag
     3961 agctgtcagg aggccgggac cttcgacggg tgggtcgcca aggtgaccga ccaggcttcc
     4021 aaggacggca tcagcggcac gccgacgtac ttcgtcgacg gcgacaaagt caccttcacc
     4081 aacggcgagg acccgaaggt caccctcggc aacctcatcg accaggccgc ctcctccgac
     4141 cagtaacccg gatgccggtc aaccactggc ggtgggctca ccgaagacgg tgccgcgcgc
     4201 acctcgcgtc gagtcggact tgtagtcggt gtactggtag gggttgtcga gcacttcctc
     4261 gtgcggcagg tcggggttca tcccaccggt ccacgcctcg tcgcccagcg tcgtccgaag
     4321 gtggtcgacg gtcgcctcga ccgtacgacg gaccgctggc aggtcgacgc cgaccaaccg
     4381 gtgcgcgtgc ttgaccacct tgccgtcgac caggaccgtg tgcacgtcac cacgctgcgc
     4441 ctggaaggcg atgtggccgt aggggttgag cagggggaac gagaccggcg aggtgtcgtt
     4501 cttgatgagc acgacgtcgg ctttcttgcc ggcttcgagc cgccccaggt cgtcccggcc
     4561 gagcgcgtgc gcaccgccgc gcgtcgccca ctcgacgaca tgctcggcac gcaggtgggc
     4621 atgcgtgacg gtgtccccct tcgcgtgtgc ctcgaggtgc tctcgggacc ggtctgcacc
     4681 gagcgtcgta cgcatggccg agaagagatc gctgctccac cacacgctgg tgtccatcga
     4741 catcgacacc gggatgtcgt gcttgcgtac ctgccaggtc ggcggatagc cctgcccggc
     4801 actttgctcg gactccgtcg acaccgagac cgaaccgccg gtggcggcga tccggtggta
     4861 ggagtcggtg gtcaacgtcg cggcatgcac gtagacgttt tcgggggtca tgaagccgtt
     4921 ctcgtgcatc aaccggatgc cgtcgtcgtt ggtggctccc cagactccgg cgtgcgtggt
     4981 caccggcagc cccagctcgc gggcgacctc gaacgccgcc ttctccggga acgccgggtc
     5041 acccgtcaca tcgaaggcca gctggaaccc gagccggtcg ccggcccccg agcgatgccg
     5101 ctccaggaac ccctgcaccg ccgggtcggc cgtccactcc cagggccccg cctggatgtt
     5161 gccgtaggcg agcacgaacc gcccaggcac cgacgtgagt gcgtcgaccg cggcctcggc
     5221 gtggtcgacg gtctgaagtc cgtgcgacca gtcgacggtg gtcgtgacgc cggcttccag
     5281 ggcgtcccac gccgagacga ggttgccggc gtacacgtcc tcggggcgaa acgccttgcc
     5341 gtgctcgagg tagtaccaga cgaagtactg cgtgagcgtc cagtcggcac cgtacccgcg
     5401 catggcggtc tgccacatgt gccggtgggt gtcgatcatc cccggcatca cgatcccgtc
     5461 ggaggcgtcg atctcctggg tgccgtccgg cacgtcgagc ccgcgaccaa ccgcggcgat
     5521 cgtgtcgtcg acgacgagca cgtcggcatc ggtgagcacg gtgtgcgagt cgtccatggt
     5581 gacgacggtg ccgccacgaa gcactaccgg cctgtccggc gagggcgcgg ccgtctcggc
     5641 gtcgggatca gacatggttc ctccagacgt acgccggtcg ggcggccggc gctccacact
     5701 ttgaccctgc gcagcagggc tgtcaatggc gctcggcaat ggcgcgcggc agtcacaccg
     5761 ttcaggcgcc gcctcccgtg acgtaccttg cttgacacgt cacaagggag acagactgac
     5821 cccggacgcg acccccaagg agcgatcggc atgaccacct catccgacgg gctcagcttc
     5881 cccacgtcgc tcgggacgtc cactcccgac cggatccagc tgctgggaca ggacctgacc
     5941 gccgacctga tgggaaacgt cggcttcggc gagctggcgt tctggttggt gaccctgcag
     6001 cgtccgacgc cgtcgcagac gcgcgtcttc gaggcggttc tcgtcgcgct ggccgatcac
     6061 gggttcacgc ccaccgcgat cgccgccagg ctcacgtacc tgtcggcccc ggactcgctc
     6121 cagggcgcgc tggccgccgg cctgctgggt ggcgggtcgc gtttcctcgg cgtcaccgag
     6181 gactgcgggg catacctgca cacggtcctg gcagcccacg aggggacgct gcccgacgac
     6241 gatgacggct gggacgctct cgcgctggct gcggtgcgtg cgcagcacgc gtccggccgc
     6301 tatgtcccgg gtctcggcca tccggtccac aaggtcgagg acccacgcac cgctgcgctg
     6361 atccggatcg cggacgagga ggggttgcgc gggccccatc tccggttgtt cgaggcgatc
     6421 ggccgcgtgc acgagcaggt gctcgggcgc agcctcccgc tcaacggagc cggcgtgtgc
     6481 ggggcggccc tcgcggacct cggcctgccg gtcgagctgc tgcgcggttt cgcactgctc
     6541 gcccggtcgg ccggcctcct cggtcagctc gcggaggaac gacgccggcc catcggcatg
     6601 gacgcctacc tggccgtcga ccgcaatgcc gtgtacgtcg accctgccga cggggacggg
     6661 cgggacgagg ccgactgatc caactgacga gggaggagga accccatggc cactgtcgcc
     6721 gcggtcatcg cctcgacgca tcatccgttc tactaccggg ccagcacctc gacgggcgag
     6781 gaccggccgc cgtttgcgga cgagtgggtc tcgaagatcg aggcgttccg cgagacgctg
     6841 acccgggccg aaccggacgt gctcgtgatg gtcggcagcg accacttcca ccagctgtgg
     6901 ctcgacaaca tgccgcagtt cctggtgggg aaggcgccgt tcttcgacgc gaacttctac
     6961 aacgaggagc gcgagttcgg tctgccgagg atgacgctgg ccggcgacga ggagctggcg
     7021 gcgtacatcc tgcgcaacgg cctcgacgcc gacttcgacc tcgccttcag caacgagctg
     7081 cggatcgacc acagcatcac ctgcccgatc atcacgctgc gccccgaggc cgacctcccg
     7141 atcgtgccga tctacaccaa catcttcgcg ccgccactcc cgcagccgaa gcgcttcgtg
     7201 caactcggtg cggcgatccg ggagatcgtc gagtcctggc cgtccgatct tcgggtcgcc
     7261 atcatcggaa
//
LOCUS       DATBFZ010000477         2330 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_4434734, whole genome shotgun sequence.
ACCESSION   DATBFZ010000477 DATBFZ010000000
VERSION     DATBFZ010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4434734"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="VH419_14520"
     CDS             complement(<1..371)
                     /locus_tag="VH419_14520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009079542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY7044879.1"
                     /translation="MRTLHIGLRVTDRGRSLAFYTAIGYEVVGSVPETPIGHLTMLKL
                     PDDEFVTVELVHDGNAVDRGTDVSHLVVQVESMGDVLGALAAHGIEPVAPGQESDDGM
                     KTTFIADPDGRRIELVQWPAG"
     gene            459..1595
                     /locus_tag="VH419_14525"
     CDS             459..1595
                     /locus_tag="VH419_14525"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY7044880.1"
                     /translation="MANAQPVHDQLHDTISRLVHRGLGVEDYWRAVSDALDGAVPSEG
                     SCLMTMDPATMLPTAEFVENGMPADAISRLVEIELREPDYTKWVDMAHEGRAAASLSD
                     VTAGQLDLSRRHREVRRPGGYSDELRVVLSSSTGTWGALTVFREKDRPYFSPSEVRLV
                     TSLTGLISDGLRRSLLLDDTCADRHDTGMLVLDPDDGVDLANPVAGNWIDELGIGTRA
                     GTTLPLVIRAVARQARAIGGQEEGHVSEAGTRPARARVRTKAGRWVIVRASLLGKGTT
                     APVAILLEAARPAEMAPLLADVYGLTPQERRVTECVAQGLPTRQIANELRRSTYTVQD
                     HLKSIFSKTGTSSRGDLIARLFFDQRAMQLAAPPAIPDEVRAVP"
     gene            2064..2291
                     /locus_tag="VH419_14530"
     CDS             2064..2291
                     /locus_tag="VH419_14530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044881.1"
                     /translation="MPFLVAQSDVAADLKFVAAASSAGPGTTMIVAMPNSPVKTVRDL
                     AGERPGPAAEPDLGCQDIRTVWFEAAGRTVP"
BASE COUNT          408 a          797 c          749 g          376 t
ORIGIN      
        1 tgacctgcgg gccactggac gagttcgatc cggcggccgt ccgggtcggc gatgaacgtc
       61 gtcttcatgc cgtcatcgct ttcctgtcct ggggcgaccg gctcgattcc atgcgcggcc
      121 agcgcgccga gcacgtcgcc catcgattcg acctgcacga cgaggtgact tacgtcggtg
      181 ccacggtcga ccgcgttgcc gtcgtgaacc agttcgacgg tgacgaactc atcgtcgggg
      241 agcttgagca tggtcagatg cccgatcggc gtctcgggca cgctaccgac gacctcgtag
      301 ccgatcgccg tgtagaacgc cagcgaccgt ccacgatcgg tcacccgcaa tccgatgtgt
      361 aacgttctca tcccgtacct ccattgcgcg tcgatgatgc tgcgaccgag gtgctcgcgg
      421 ctatccccac atttctgggg gttcggctaa gttgagtcat ggccaacgcc cagccggtgc
      481 acgaccagct ccacgacacc atctcgcggc tggtccaccg cgggctgggc gtggaggact
      541 actggcgcgc ggtgagcgat gcactggatg gcgcggtgcc gtcggagggc tcctgcctga
      601 tgacgatgga cccggccacg atgctgccga ccgcggaatt cgtcgagaac gggatgcccg
      661 cggacgcgat cagccggctc gtcgagatcg agttacgcga acccgactac accaagtggg
      721 tcgacatggc gcacgagggt agagccgcag ccagcctcag cgacgtcaca gcgggccagc
      781 tcgacctcag ccggcgtcac cgggaggtcc gacgccccgg tggttactcc gacgagctcc
      841 gagtcgtcct gtccagcagc accggcactt ggggcgcgct caccgtgttt cgcgagaagg
      901 accgtccgta cttctcgccc tccgaggtac ggctcgtcac atcgctgacc ggtctgatct
      961 cggacgggct gcgccggtcg ctgctactgg acgacacctg cgccgaccgc cacgacaccg
     1021 gaatgctcgt tctggatccc gatgacggcg tcgacctcgc caatccggtg gccgggaatt
     1081 ggatcgacga acttggcatc ggcacgagag caggcacaac gctcccactg gtgatccgtg
     1141 cggtcgccag gcaggcccgt gccatcggcg gccaggagga gggccacgtg agcgaggcgg
     1201 gcaccagacc agccagagcg cgtgtgcgta ccaaggcggg gcgttgggtg atcgtccggg
     1261 catcgctact gggcaagggc acgaccgcac cggtcgcaat cctgctcgaa gcagctcgcc
     1321 cggccgagat ggcaccactg ctagcggacg tgtacggcct caccccccaa gaacgacggg
     1381 tcaccgaatg tgtcgcgcaa ggcctgccga ccagacagat cgccaacgag cttcgaaggt
     1441 cgacctacac cgtccaagac catctgaagt cgatcttctc taagactggc acgagctccc
     1501 gcggagacct gatcgcccgc ctgttcttcg atcagcgcgc catgcaactt gcggctccgc
     1561 cggcaatccc agacgaggtg cgcgccgttc cgtaacggcc cgtctccaac gtctccctca
     1621 gggccgcgca taacgcctcg cggcgacgaa cccggttggc ctccagggca agcacggcca
     1681 gcacgaaccg ctgcatgcga acccgcacgc tgaccggtcg gccggagacg cacaggcgca
     1741 ccggtccgag cagctgaaag tcgatctccc gcggggcatc catcaccggc ccccgactgg
     1801 cgggtgcacc gaccggttga accgaacgcc agccatgatc gcaacatcgt tgaccgcaac
     1861 atcaccacac ccggcggtca ccgttcttgc atcgcgtcgc gccgtcgtgc tcgcgatcgg
     1921 gcctgcgcag tggtatggcc accagtcgag agtggagcag caacatgggc gactcgaagc
     1981 catcccggga aatcgaggtt ggtgctgtca tttcggtaca tcagacttcg cagggcattg
     2041 tccgacatgc gtacagctgc tggatgccgt tcttagtcgc tcagagcgat gtcgccgcgg
     2101 acctgaagtt cgtggccgcc gcgtcgtccg ccggtccggg cacgaccatg atcgtggcga
     2161 tgcccaactc gccggtcaag acggtgcgtg acctggccgg agagcggccc ggtcccgcag
     2221 ccgaacccga cctgggctgc caggacatac gcacggtctg gttcgaggcg gccgggcgaa
     2281 cggtgccgtg atcggcccga aaccgtgatg tcaggccggc cttctactgg
//
LOCUS       DATBFZ010000478         2010 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_5746158, whole genome shotgun sequence.
ACCESSION   DATBFZ010000478 DATBFZ010000000
VERSION     DATBFZ010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5746158"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1092)
                     /locus_tag="VH419_14535"
     CDS             complement(<1..1092)
                     /locus_tag="VH419_14535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012923294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HEY7044882.1"
                     /translation="MATTTEVPEAEPTRVVGQPGRHGEPHIHHDAGPVALLGTTIDGD
                     ELPRVRRRLTPPSYRSDPFWGWVAPLIITVCAGILQFWSLTTPHDITFDETYYAKDAY
                     SLLVKGYAESFVNNPDTDQNEADDLINSGDTKPSDLFTDTPNKVVHPEVGKWMIAVGE
                     WLFGLTPLGWRFSAALTGTLMVLVLCRLVRRLTGSTLLGCIAGTLLALDGLHFVMSRF
                     ALLDIFLAFWLVCAVHCLVADRDWGRAKIARRYERSPTQSPGDFGPIRGFLFRPWRIA
                     AGVCFGLAAGTKWTTIFVLAGFVLLVWAWDTGSRRSIGVRSAPLKSLVADMIPAFFSI
                     VMVAVVVYVVSWMGFLLHAQKFEDAFGHDT"
     gene            1127..1981
                     /gene="rsmI"
                     /locus_tag="VH419_14540"
     CDS             1127..1981
                     /gene="rsmI"
                     /locus_tag="VH419_14540"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002513264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEY7044883.1"
                     /translation="MSEDAGMTLVLAATPIGRIEDAPRRLSTELAGADVIAAEDTRRL
                     RRLLTELGVSTRARVVSYFDGNEGARTPVLLEALTAGERVVLVTDAGMPSVSDPGYRL
                     VAAAVAAGVSVTAVPGPSAVLTALAVSGLPTDRFCFDGFLPRKAGERRRRLEELTDEP
                     RTMVFFEAPHRTAETLSAMAAVFGSDRRAAVCRELTKTYEEVRRATLVELAGWAEEGI
                     RGEVTIVVAGASISEPAGDLDAADLRELVEAGVESGLSRKDAVADVARRTGTPRKTVY
                     AAAHSVEP"
BASE COUNT          352 a          663 c          698 g          297 t
ORIGIN      
        1 ggtgtcatga ccgaaggcgt cctcgaactt ctgcgcgtgg agaaggaagc ccatccacga
       61 cacgacgtac acgacgaccg cgaccatgac gatggagaag aacgccggga tcatgtcggc
      121 gaccagtgac ttgagcggcg ccgacctgac gccgatcgat cgccgcgacc ctgtgtccca
      181 cgcccagacc agcaggacga acccggcaag aacgaagatc gtcgtccact tcgtgccggc
      241 cgcgaggccg aagcagacac ccgcagcgat ccgccacggc cgaaacagaa aaccccggat
      301 cggcccgaag tcgcctggtg actgcgtggg ggaccgttcg taccggcggg cgatcttcgc
      361 gcgtccccag tcccggtcgg cgaccaggca atgcacggcg cagaccagcc agaatgcgag
      421 gaagatgtcg agcagcgcga accgcgacat cacgaagtgc agcccgtcga gggccagcaa
      481 tgtccccgcg atgcaaccaa ggagagtcga gccggtcagc cgtcgtacga gccggcacag
      541 caccagcacc atcaaggtac cggtgagtgc cgccgaaaaa cgccagccca gcggcgtcaa
      601 cccgaacagc cactcgccga ccgcgatcat ccacttgccg acctcagggt gcaccacctt
      661 gttcggcgtg tccgtgaaca ggtccgacgg tttcgtgtcg ccggagttga tgaggtcgtc
      721 ggcctcgttc tggtcggtat cggggttgtt cacgaacgac tcggcgtacc ccttgacaag
      781 gagggagtag gcgtccttgg cgtagtaggt ctcgtcgaag gtgatgtcgt gcggcgtggt
      841 caggctccag aactgcaaga ttccggcgca gaccgtgatg atgagcgggg cgacccaccc
      901 ccagaacggg tcgctgcgat acgacggtgg ggtcagccgg cggcgcacgc ggggcagctc
      961 gtcaccatcg atcgtcgtac cgagcagcgc gaccggaccg gcatcgtggt ggatgtgtgg
     1021 ctcgccgtga cggccgggct gcccgacaac ccgggtcggt tccgcctccg gaacctcggt
     1081 cgtcgtcgcc acgcccgcaa tggtaggtga ccgaacaacg cgccggatga gcgaggatgc
     1141 gggcatgacg ctcgtgctcg ccgcgacccc gatcggacgc atcgaggacg cgccccgacg
     1201 gctgtcgacc gagctcgccg gcgccgacgt catcgccgcc gaggacaccc ggcggctcag
     1261 acggctgctc accgagctcg gcgtttccac gcgtgcgcgc gtcgtgtcgt acttcgacgg
     1321 caacgagggt gcccgcacac ccgtgctcct ggaggcactg accgcggggg aacgggtcgt
     1381 gctggtgacg gatgcgggca tgccgagcgt ctcggacccg ggctaccggc tggtggccgc
     1441 agccgtcgcc gctggcgtat cagtgactgc cgttcccggc ccctccgcag tgctgacggc
     1501 gttggcggtg tcgggcctgc ccaccgaccg gttctgcttc gacggcttcc tgccgcgcaa
     1561 ggccggagag cgacgccgtc ggctggagga actgacagac gagcctcgga cgatggtctt
     1621 cttcgaggca ccgcaccgga ccgccgagac gctgtctgcg atggcagcgg tgttcgggtc
     1681 agaccgtcgt gcagcggtgt gccgggagct caccaagacg tatgaggagg tccgccgcgc
     1741 aacgctggtc gagcttgccg gatgggccga ggaagggatc agaggcgagg tcacgatcgt
     1801 ggtcgccggc gcatcgatct ccgaaccagc cggggatctc gatgcggctg acctgcgtga
     1861 gctggtcgaa gcgggtgtcg agagcgggct gtcccgcaag gacgcggtgg ctgacgtcgc
     1921 ccgccgtacc ggcacacccc gcaagaccgt ctacgccgcc gcccactccg ttgaaccgtg
     1981 agttgtggtt cgtcgagccg tgagttgtgg
//
LOCUS       DATBFZ010000479         4280 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_6088872, whole genome shotgun sequence.
ACCESSION   DATBFZ010000479 DATBFZ010000000
VERSION     DATBFZ010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 4280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4280
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6088872"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(76..429)
                     /gene="hisI"
                     /locus_tag="VH419_14545"
     CDS             complement(76..429)
                     /gene="hisI"
                     /locus_tag="VH419_14545"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009074964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HEY7044884.1"
                     /translation="MDETPDTVDAAADVAAGLRFDASGLLPVVVQEATSKDVLMLAWM
                     DAEAVQRTRATGRATYWSRSRREHWVKGETSGNVQYVRELRVDCDGDTLLLVVDQTGP
                     ACHTGTTTCFDEGLI"
     gene            complement(434..1369)
                     /locus_tag="VH419_14550"
     CDS             complement(434..1369)
                     /locus_tag="VH419_14550"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY7044885.1"
                     /translation="MTSLLTRRTELDDVDGRPVWSFVEGETLVRDHLAWTRLGIGTRC
                     ETWLAWSEPLWSPAIVKLTRPEQVTHPRGRAAVAREADALGAVRHPGLPRLLYDGRAG
                     QVPYLVAQYVDGPDLDHVLEESGPLDEAEAVMLVAQVLSVVRSLHAAGFAHLDLKPEN
                     LILQNGCAVLVDLGSSRRIGHRQPSGRPIGTTGYAAPDLERGDDIAASMDVYGLGTVF
                     AEALTGRRLFDPHAPAADRPHPELPGSVSPGVHRLLGDMLRAEPQDRPTIDELLLALP
                     ALMGAQDSDELLWPSWASRALSARPGPATASRAGQ"
     gene            complement(1489..3336)
                     /locus_tag="VH419_14555"
     CDS             complement(1489..3336)
                     /locus_tag="VH419_14555"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY7044886.1"
                     /translation="MRGPSSWSVAPVLRGDIGALAGASALGTLAVGAQLLQPWPISIV
                     VDHAIEGRPFSGPLAGLQSLSPSGVLLLAALATIGLAALAGLLDGWSMRLAEGATERI
                     GGRLRQHVFGRVLGLSLRWHDRHPSGEVVSRLTSDVGRVMDAISVTTGLIPDAILLVA
                     VLTVLATLDVGLALMGLLAVPVLAGLAVRQRRRLRAAEVAARTARGEMSANVTDLLRN
                     IRAVQALGATSRARSSFGVHNAKLVNREVEAVRTAARWTPRADIVLSVGTGAVLAIGG
                     HRVLAGALTTGTLLVVLSYLRDLYRPVRSLARASAVYAKARASDDRLQEVLTSTERVP
                     DGPTRGGRLPAGHADIRLRQVSFSYAAGRRVLDRFDMTIRSGEVVCITGPSGVGKSTL
                     LSLLLRLYDPDAGAITLAGVDIRALSLDTLRSNIAFVPQDPWLLDATIRDNIAFAATT
                     IDEAAVVAASRAALVQEFADRLPLGLDTRIGEGGAMLSGGQRRRVAIARAFATAAPVL
                     LLDEPTASLDQDAATSVLAAIQSAAAGRTVVVVTHDPQVGAIADRVVRLPATSSAPTE
                     PRMVSTSTRRTDDARKEVSQHAHTCTDHPGRGESPRKEPVAEPVALSQP"
     gene            3462..4145
                     /locus_tag="VH419_14560"
     CDS             3462..4145
                     /locus_tag="VH419_14560"
                     /inference="COORDINATES: protein motif:HMM:TIGR03085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03085 family metal-binding protein"
                     /protein_id="HEY7044887.1"
                     /translation="MDSAPDEHDLRHAYETPVASGPGPAVSEVERALLVETLLLVGKD
                     SPTLCTGWDTHHLAAHLVLRESNPVGSVRAALPKLSDDAVDEVVRRTPFERLVEQFRT
                     GPPALSFYRIPGADRRLNAIEHLIHHEDVRRAQPQWTRRALPPWVHEQIWHGLRYGGK
                     VMLRHSPVPVLLARSDTDETLVARKGAPAVVVRGLPVELALFASGRQQVAGVDLDGDP
                     TDVQRLRSA"
BASE COUNT          682 a         1477 c         1477 g          644 t
ORIGIN      
        1 ggctcgagca cgtgaccgag gaggagtaca gcagcgcgac gtaggccgct gggaccaccg
       61 ccggtgtccc cgatgtcaga tgagtccttc gtcgaagcag gtcgtggtgc cggtgtgaca
      121 agcaggtccg gtctggtcca cgacgagcag gagagtgtcg ccgtcgcagt caacccggag
      181 ctcacggacg tactgaacgt ttcctgacgt ctcgcccttc acccagtgtt cgcgacggct
      241 gcgactccag tacgtcgctc gaccggtcgc gcgggttcgc tgtacggcct cggcgtccat
      301 ccaggccagc atgagtacgt ccttcgaggt cgcttcctgc acgacgaccg gcagcagccc
      361 gctcgcgtcg aacctcaggc cggctgcgac gtctgcggcg gcgtcaaccg tgtcgggtgt
      421 ctcgtccacc tgatcattgt ccggcacgcg acgccgtggc cggaccgggc cgagccgaca
      481 aggcgcgcga cgcccacgac ggccacagca gttcgtcgct gtcctgcgca cccatcagcg
      541 cgggcagagc gagcagcagc tcatcgatgg tcggacggtc ttgtggctcg gctcggagca
      601 tgtcgcccag cagccggtgg acgcccggcg agacggatcc ggggagctcg gggtggggcc
      661 ggtcagctgc cggcgcgtgc gggtcgaaca gccggcgtcc ggtcaaggcc tccgcgaaca
      721 ccgtgccgag accgtagaca tccatcgagg cagcgatgtc gtcaccgcgc tcgaggtcgg
      781 gagcggcgta cccagtcgtg ccgatgggac gacccgacgg ctgacggtga ccgatccggc
      841 ggctggagcc caggtcgacg aggacggcgc agccgttctg caggatgagg ttctcgggct
      901 tgagatccag gtgcgcgaaa ccggctgcat gcaggctgcg aacgacggac agcacctgcg
      961 cgaccagcat gacggcctcg gcctcgtcga gcggccctga ctcctcgagg acgtggtcga
     1021 gatcgggccc atcgacatac tgcgcgacga ggtacgggac ctggccggct cggccgtcgt
     1081 acaagagtcg tggcaaccca ggatgcctga cggcacccaa cgcgtccgcc tcgcgggcca
     1141 ccgcagcccg accgcgtgga tgcgtcactt gctcgggccg tgtcagtttc acgatggccg
     1201 gtgaccagag cggctccgac caggccagcc aggtctcgca tcgggtgccg atgccgaggc
     1261 gggtccacgc gaggtggtcc cggacgagcg tctcgccctc gacgaacgac cagaccggcc
     1321 tgccgtcgac atcgtcgagt tccgtcctac gtgtcagaag tgaagtcatc atgctctcgc
     1381 gtgctcaggt cgcggactcg tatggttgga ggtagggcgc ggccgcccga gtgcgcaccc
     1441 tggggtgaac ggcttcgggc ggccgccgct ggtcagtcct tgctacggct acggctgcga
     1501 gagcgcgacc ggctccgcga ccggctcctt ccgtggtgac tcaccacggc ctggatgatc
     1561 cgtacacgtg tgcgcatgct ggctcacctc ctttcgggcg tcatcggtcc tacgggttga
     1621 ggtggacacc atccgtggct cggttggtgc cgaacttgtg gcgggaagcc gtacgacgcg
     1681 gtccgcgatc gcaccgacct gcggatcgtg tgtcaccacg acgacggtcc ggcccgcggc
     1741 agccgactgg atcgcggcca gcacagaagt ggcggcgtcc tggtcgagtg aggcggtcgg
     1801 ctcgtccagc agcagcaccg gggccgcggt ggcgaaggcg cgggcgattg cgacacgacg
     1861 gcgctgaccg cccgaaagca tcgcaccgcc ctcaccgatc cgcgtgtcga gaccgagggg
     1921 aagccggtcc gcgaactcct ggacgagcgc cgcgcggctc gcggcgacca ctgccgcttc
     1981 gtcgatcgtc gtggcagcga acgcgatgtt gtctcgaatg gtcgcgtcga gcagccacgg
     2041 gtcctgaggc acgaacgcga tgttcgaccg cagtgtgtcc agggacagtg cccggatgtc
     2101 gacgccggcc agggtgatcg cgccggcgtc cgggtcgtac aagcggagca gcaggctcag
     2161 cagcgtgctc ttgccgactc cgctcggtcc ggtgatgcac acgacctcgc cgctgcggat
     2221 cgtcatgtcg aaccggtcga gcacgcggcg gccggccgca tagctgaagc tcacctgccg
     2281 gagtcggatg tcggcatgcc cagccgggag ccgaccacct cgggtgggtc cgtccgggac
     2341 ccgctcggtc gacgtgagca cctcctgcag cctgtcgtcg ctggctctcg ccttcgcgta
     2401 gaccgcagac gctcgcgcga gcgaacgcac cggccggtac aggtcgcgca ggtagctcag
     2461 gaccaccagc agcgtcccgg tggtcaacgc accggcaagt acccggtgtc cgccgatcgc
     2521 gagaaccgca ccggtaccta cgctcagcac gatgtcggcc cggggggtcc agcgtgcggc
     2581 cgtacgtacc gcctcgacct cgcggttgac gagcttggcg ttgtgtacgc cgaaggagct
     2641 ccgcgctcga ctcgtcgcac cgagcgcctg gacagcccgg atgttgcgga gcaggtctgt
     2701 cacgttggcc gacatctcgc cacgggcggt tcgtgcggcg acctccgctg cccggagccg
     2761 gcggcgctga cgtactgcga gcccggcgag caccgggacc gccagcagac ccatcaacgc
     2821 cagtccgacg tccaacgtgg ccagcaccgt cagaacggcg acgaggagga tcgcgtccgg
     2881 gatcaggccg gtcgtgaccg agattgcgtc catcacacga ccaacatctg acgtcagccg
     2941 cgacaccacc tcgccgctcg gatggcggtc gtgccagcgc aacgacagcc cgaggacccg
     3001 gccgaagacg tgctggcgga gccgtccacc gatccgctcg gtcgcaccct cggcgagacg
     3061 catgctccat ccgtccagca acccagccag cgcggccaag ccgatcgtgg ccagcgccgc
     3121 cagcagcagg acgccggacg gtgacagcga ctgcagaccg gcgagcggcc cggagaaggg
     3181 ccggccctcg atcgcgtggt cgacgacgat ggagatcggc cagggttgga gaagctgagc
     3241 cccgacggcg agcgtgccga gggcggaggc gccggcgagc gcaccgatgt cgccacgcag
     3301 gacgggtgcg acactccagc tgctcggtcc tctcacgtgg acgacattgc cccaattgcg
     3361 gcgcaggttg ggattgtcct tatgtcctac gaccccgcat cgatcgggta ggacccgagt
     3421 acaggtcagg cggaacccgc acggccaact agatttgacg cgtggactcc gcacccgacg
     3481 aacacgacct tcggcacgcc tacgaaacgc cggttgcatc cggtcccggc ccagccgttt
     3541 cggaggtcga gcgggccttg ctggtcgaga cgctcctgct ggtgggcaag gactcgccaa
     3601 ctctgtgcac cggctgggac acgcatcacc tggctgccca cctggtcttg cgggagtcca
     3661 atccggtggg ctcggtcagg gccgcgctgc ccaagctgag cgacgacgcg gtcgacgagg
     3721 tcgttcggcg tactccgttc gagcggctgg tcgagcagtt ccggaccgga cccccggcgc
     3781 tgtcgttcta ccggatccca ggcgccgatc gacggctcaa cgcgatcgag catctgatcc
     3841 accacgagga cgtgcggcgg gcacaaccgc aatggacacg tcgcgcgctg ccgccgtggg
     3901 tccacgaaca gatctggcac ggactgcgat acgggggcaa ggtgatgttg cggcacagtc
     3961 cggtgccggt gctgctcgcg cgttcagaca ccgacgagac cttggtcgcc cgcaagggcg
     4021 caccggccgt cgtcgtgcgc ggtctgcctg tggagctggc gttgttcgcg tccgggcgcc
     4081 agcaggtggc cggtgtcgac ctggacggcg atccgaccga cgtccaacga ctccgctccg
     4141 cctagtaccc cgtcaaccaa cattcgccct gttgcgcgcc gcccgggcgg cgatctgcgg
     4201 cgttatcgtc ggtcaacgat gctgcgcatc gcctccctcc tctgccttgc agctcatcca
     4261 cccggacacc gctcacgacg
//
LOCUS       DATBFZ010000480        21800 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_5749686, whole genome shotgun sequence.
ACCESSION   DATBFZ010000480 DATBFZ010000000
VERSION     DATBFZ010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 21800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21800
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5749686"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1956)
                     /gene="cutA"
                     /locus_tag="VH419_14565"
     CDS             complement(<1..1956)
                     /gene="cutA"
                     /locus_tag="VH419_14565"
                     /EC_number="1.2.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic carbon-monoxide dehydrogenase large
                     subunit"
                     /protein_id="HEY7044888.1"
                     /translation="MTTKMFGQRIPRVEDPRLLSGRGTYLDDLGHDALAAAFVRSPHA
                     HARIVDIDVSAALDVDGLVAIYTHEDLDGRTAEPLPLLIPHPTLTQGRTPYALAKDEV
                     NHVGEAIVMVVATDRYVAEDAAGLVNVSYDFLPPVVGLENARDGEALVHDDVPGNVAA
                     TMIQEVGDAAAAIAAAPHRGSLSLSIERSASMPMEGKGVYARWDANDQSLLVQSSTQT
                     STSVRQAIAAKLSLPVDRVEVVTPDVGGGFGVKIVHPWPEEVLVPWAAIRLGHPVKWT
                     EDRREHFVSAAHERGQLHEVEFGYDDDGTLRGLSVRFWHDNGAYTPYGLIVPIITSTQ
                     LLGPYKPGAYRVEFQSLYTNTVLVTPYRGAGRPQGCFVMERVMDAIAHERGLDRTVVR
                     ERNFIMPDEMPYDHGLLFQDGRPLIYDSGDFPATLGKIKALVGWDDFPAYQEKARAEG
                     RRVGIGLACYVEGTGVGPYEGGHIRVETDGTVVVSTGLTTQGQGHQTMLAQIVSDELG
                     VPIARIRVTTGDTRRFKYAVGTFASRTAVMSGSAVALTARRVREKAMRIAANALEAAV
                     DDLEIVDGHVRVKGSPTSEIDLGAVAVLSNPLRYAFDEAAARATQFAAPADPDKPPVE
                     RGEAPGLEGTDYYSPPRSTFANGMHAVV"
     gene            complement(1953..2471)
                     /locus_tag="VH419_14570"
     CDS             complement(1953..2471)
                     /locus_tag="VH419_14570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEY7044889.1"
                     /translation="MSEQLHAITLVINDVLYPLAVPARRLLSDAIRHDAGLTGTHVGC
                     EHGVCGACTVLLDGVPVRSCLVLAVTADGSRITTTEGCLLPNGGLGPVQQAFQDCHAL
                     QCGFCTPGFITTITAYLETNPDPTEEEAREAIGGNLCRCTGYQNIVAAVLRAAEIRHQ
                     ATGETATGEPAR"
     gene            complement(2468..3349)
                     /locus_tag="VH419_14575"
     CDS             complement(2468..3349)
                     /locus_tag="VH419_14575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEY7044890.1"
                     /translation="MKPAPFDYHRAASVDDAVEALAATGGKVLAGGQSLVPILSMRLA
                     APPALVDINHIDRLDTIEVGDGGVRVGALVRHAGLEHDRAAYDANPLLRDALSHVAHA
                     TIRNRGTTVGSLVHADPAAEMPAVLALLGGYVEAVSRGRGRRRIEASDFFVGPLESAL
                     TDAEMAVSAYFPDPPEGAGTAWLELSRRRGDFAMVGVGAMVRLEAGVVAAASVSLVSV
                     GPVPVVVDLTSAVTGDTADESDWADAVDLVDQAIDPETDIHASADYRRELARVLSRRA
                     LTKAAAAARGRTIGGGT"
     gene            complement(3389..4882)
                     /locus_tag="VH419_14580"
     CDS             complement(3389..4882)
                     /locus_tag="VH419_14580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HEY7044891.1"
                     /translation="MAFGWTVVHGGRTPPPGEAVAPDERLSWPRTVGLGAQHVVAMFG
                     ATFVFPIVMGLNPQLAIMMSGIATICFLLIVSGKVPSYLGTSASFVGGVAAIRAQGGD
                     SADVTGAILVAGLVLLLIGVLIHYAGSHVLHAVLPPAVTGAVVMLIGFNLAPVVADIY
                     WPQDQWIALITMAFVIVFAVGVRGFLGRIAIFVGLIFGYVISWIADSFSQVNSVLPGQ
                     NLLGPDGKPCSPEGLYCVASPFDHDRISWDAVKSADWIGFPPHTENGVVGWHWPSFSL
                     TFTLLVLPAVIALIAENTGHVKAVAEMTRRDLDPVMGKAIAADGFGTALASSVGGSPT
                     TTYAENIGVMAATRVYSTAAYYVAAVVAILFGLSPKFGALVSSTPGGVLGGITVVLYG
                     MIGLLGAKIWKENNVDLGNPVNLVPLAAGIIIAIGDTSLEITDDFTLSGIALGTIVVI
                     VFYHLARAIAPAHLRVDDPGTMISVGESGLHEVEEEPGGSHRRDPDS"
     gene            5866..6333
                     /locus_tag="VH419_14585"
     CDS             5866..6333
                     /locus_tag="VH419_14585"
                     /inference="COORDINATES: protein motif:HMM:NF022834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HEY7044892.1"
                     /translation="MTFTVARWWRPAKPRHHALAEAVDPAGVRRLVDTWPDLLGRGTG
                     LTPYGDDVLCGALVTLSAVQHPDFAPLASDISAASLDERTTATSAALLRAACEGWCID
                     ELAWHLAALATPDPASYAAASRRRLHAVGSSSGRGLLEGVGLVLGDRAGRVAA"
     gene            6330..7790
                     /locus_tag="VH419_14590"
     CDS             6330..7790
                     /locus_tag="VH419_14590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FdrA family protein"
                     /protein_id="HEY7044893.1"
                     /translation="MTRAVEIRRGAYQDSVSLMQLSRELTGRPDVTNVQVAMATELNL
                     ELLEQMGFEVPAEATPNDMVVAIEAPDDEAIAATRSALDEALTRTPARTDGWDDATPP
                     APTLGAAARRVDASLALVSTPGRVAVVDAADALAAGLDVMVFSDNVSLDHEVALKDWA
                     AELGLLVMGPDCGTAVVDGVGLGFANVVRPGPVGIVAASGTGAQQLLALLDGADVGVK
                     HCLGVGGRDLSAPVAGRSTLAALDRLAADDDVETIVVISKPPAPDVAARVTEHAKAFD
                     KPAVIGYLGPGQPDLTTTAQRVAEAAGAAWTPPRRWGPQSPPPRDGFLRGLFSGGTLC
                     DESMVLAVEHLGRVASNIPLDGQSTLGHDLAGAGHTCIDFGDDQLTAGRPHPMIDPSL
                     RLDRLERELADPECAVVLLDVVLGHGAHPDPATDLSDVIAGSTTPVVVSLVGTRDDPQ
                     GLEAQATRLAEAGAVVHASNAAATREALAIIEGPGQ"
     gene            7787..9226
                     /locus_tag="VH419_14595"
     CDS             7787..9226
                     /locus_tag="VH419_14595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HEY7044894.1"
                     /translation="MSSDPISAAAVVTAGAALLEESLRGQGVTVEPVAWTPPVAGTET
                     ALAAVMLDRRRAAANTEAVRRMTSARATLVDVRPAATALGLERGEFLHAGPPISWDRA
                     SGPLRGALIGAVLFEGLAATAEEAEALLSSGACRLEPCHHRGAVGPMAGVVSPSMWVF
                     ELRDEVHEATSWCSLNEGLGKVLRYGAYSPDVIDRLHWMTDVLGPALGAATRARGPID
                     ITAIIAQMVQMGDEGHNRNRAGTLMLLKEWLPDLITSGLPTADLAEVVRFVAGNDHFF
                     LNLVMPACKLMTVAASGIPGSSVVTTMARNGTDFGIRVSGTGDRWYVGPAQLADGLYL
                     GNYGPDDANPDIGDSAITETAGIGGFAMATAPAIVRFVGGSVPDALATTRRMYDLCVG
                     ENPGWAIPIMDFRGTPTGIDVTSVLRSGQLPQINTGMAGKVAGTGQVGAGLVTPPMEC
                     FVAAIEGLAEVATASRHADKLQPNLPPRR"
     gene            complement(9269..9763)
                     /locus_tag="VH419_14600"
     CDS             complement(9269..9763)
                     /locus_tag="VH419_14600"
                     /inference="COORDINATES: protein motif:HMM:NF022216.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEY7044895.1"
                     /translation="MPADSDATRDGATPYEVHLPRTYRPLGARVATATAAVAIVVAMT
                     FLWLMLPGNVQDDFTTFQRITLVAFFVAILVLLNAIFRTSARATESGLEVVNGYRRRF
                     LEWGQIVRISLSPNRPWALMDLDDGTTQAVMAIQTSDGARAIRSAKELARVIARHTPT
                     DHDT"
     gene            complement(9747..10592)
                     /gene="hisG"
                     /locus_tag="VH419_14605"
     CDS             complement(9747..10592)
                     /gene="hisG"
                     /locus_tag="VH419_14605"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015011627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEY7044896.1"
                     /translation="MLKIAVPNKGSLADPATQMLREAGYRQRRDERDLVMSDDMNDVE
                     FFYLRPRDIAVYVGEGTLDVGLTGRDLVLDSRAPVVERLTLGFGESTFRFAAPVGTFA
                     GADDLDGARVATSYAGILEDWFKERAVNAKVVRLDGAVESAVRLGVADAIADVVSTGN
                     TVRQAGLEFFGEPILTSETVLVTRDNTPDPNGFEVFLRRIQSVLVARRYVMMDYDCGV
                     EDVEAACALTPGIESPTVAPLHRDGWVAVRSMVPRDDAQRVMDELYELGARGILVTDI
                     HACRL"
     gene            complement(10603..10866)
                     /locus_tag="VH419_14610"
     CDS             complement(10603..10866)
                     /locus_tag="VH419_14610"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HEY7044897.1"
                     /translation="MKTFDTLYAELATKVAERTPGSGTVVAVDAGVHAIGKKLVEEAA
                     ESWMAAEHESKERTAEELSQLLYQAQVMMLACGLTPDDVYAHL"
     gene            complement(10936..11412)
                     /gene="ribH"
                     /locus_tag="VH419_14615"
     CDS             complement(10936..11412)
                     /gene="ribH"
                     /locus_tag="VH419_14615"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020390966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEY7044898.1"
                     /translation="MSGTGQPTLTSIDAAQLRVVVVAAEWHATVMDGLIAGAERALSE
                     ANVAEYRVVRVPGSFELPIVSQRVASDGADAVVALGVVVRGGTPHFDYVCDAVTAGLT
                     RVALDTGVPVGFGVLTCDTEEQALDRAGLEGSKEDKGYDATHAALSTALTLAALRP"
     gene            complement(11409..12689)
                     /locus_tag="VH419_14620"
     CDS             complement(11409..12689)
                     /locus_tag="VH419_14620"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HEY7044899.1"
                     /translation="MRLDAIERAITDVAAGKPVVVVDDEHRENEGDLIFAASKATTKL
                     LAFMIRYSSGVICVPMEGPALDRLAIPLMTPHNRERMRTAYTISVDARDGVTTGISAA
                     DRAHTIRVLVDSATEPFEVVQPGHVFPLRYRDGGVLVRPGHTEASVDLARLAGLTPAG
                     AISELVNDDGTMKRGTQLREFADEHGLALISIADLIRYRRRHERQVQRVAETRLPTEF
                     GTFQALGYRNLVDGSEQIALVKGDISDGRDVLVRVHSECLTGDVFASQRCDCGPQLHA
                     CLEAVSREGRGVVVYLRGHEGRGIGLLHKLKAYTLQDDGLDTVDANLDLGLPADARDY
                     GTGAQILHELGITSVRLLTNNPDKRTALEGFGVTVAERIAVPGMTHEENRRYLATKRD
                     RMGHELSHLDLGPVSTAADDEVDSSENRIGGPPA"
     gene            complement(12749..13450)
                     /gene="pnuC"
                     /locus_tag="VH419_14625"
     CDS             complement(12749..13450)
                     /gene="pnuC"
                     /locus_tag="VH419_14625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017933560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HEY7044900.1"
                     /translation="MSLFQTAYDAHLTISGHEITWREIIGNAFGFASAIGGLKRRVWA
                     WPVGIIGNVLLFTVFMGTAVVGDQHIPLLGQAGRQIFFIIVSVYGWHRWRQTRHRSAQ
                     HGPAVIPRWATGRERAAYLAAAAVAVLVLTYVFRWIGAGWPAPSWYYVADAWIFVGSI
                     LATYAMARGWVDFWLCWLAVDLVGVPELIHFQLYPSAMLYAAYGVLVIYGFFSWRRIA
                     RNERLADPTAEMVGL"
     gene            complement(13447..14073)
                     /locus_tag="VH419_14630"
     CDS             complement(13447..14073)
                     /locus_tag="VH419_14630"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008359332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEY7044901.1"
                     /translation="MFTGIIEELGEVVALDQLGDSIRLTVRGPRVVSDAGHGDSIAVN
                     GCCLTVVEWDDERFTVDVMAESLDRTSLGALTQGSRVNLERAVGPQDRLGGHIVSGHV
                     DGVGTIVRREPSEHWDVVTIALPADLARYVAMKGSITVDGVSLTVSGLVDEPEPAFQV
                     SLIPTTLELTTLGFKQPGDPVNLEVDVIAKYVERLLDVPDGASTKERP"
     gene            complement(14073..15116)
                     /gene="ribD"
                     /locus_tag="VH419_14635"
     CDS             complement(14073..15116)
                     /gene="ribD"
                     /locus_tag="VH419_14635"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020390962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEY7044902.1"
                     /translation="MATDSEVLAMTQAFALACTPGVPTGPNPRVGAVVLDAGGRVVGE
                     GYHRGVGSPHAEVAALQAAGELAAGGTAVVSLEPCNHTGRTGPCTQALIAAGVRRVVF
                     AQHDLNPIADGGAEVLRHAGIDVEGGVLAAEAEAINEIWSFAVRNQRPFVTWKLAATL
                     DGRSAAADGSARWITSGPARADVHRQRALCDTVLVGTGTVLADDPQLNVRDEHDDPLP
                     VEHQPLRAVMGLRPIADGARVRDATAPTTFLATRDPQVALAKLYADDRQHVFLEGGPT
                     LAAAFVRAGLVDELIAYVSPALLGAGLSAVGDLGVTSIRGIRRFGLLEVVQVGDDVRL
                     TMRSPTRPAEEDR"
     regulatory      complement(15244..15371)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(15392..16069)
                     /gene="rpe"
                     /locus_tag="VH419_14640"
     CDS             complement(15392..16069)
                     /gene="rpe"
                     /locus_tag="VH419_14640"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEY7044903.1"
                     /translation="MAKLTTPLIAPSILSADYANLEAAARSVPGADWLHVDVMDNHFV
                     PNLTIGPPVVEALAKVAEQPLDCHLMIEDPDRWAPAYVEVGASSVTFHVEACAAPVRL
                     ARELRAMQARAGMALKPATPVEPYEAMLPELDLLLIMTVEPGFGGQQFLDGCLPKIRR
                     AREMIDRYGGEVWLQIDGGVAADTIERCAEAGADFFVAGSAVFGSDDPDAAVKSLRTR
                     AQTSVSY"
     gene            complement(16207..17628)
                     /locus_tag="VH419_14645"
     CDS             complement(16207..17628)
                     /locus_tag="VH419_14645"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020390702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEY7044904.1"
                     /translation="MPDAPRRARARTPVDPARQVAYDTLRAVEIDDAYLNLTLSRLLS
                     QRRIAGRDAAFATELAAGTARLQGSYDLILDALATGGVAGLQPAVLTALRLGVHQLLS
                     MRVPAHAAVGTTVELARTEVGERPVRLVNAVLRRVADRDLGSWLSALAPARDADLVGH
                     LAAVHAHPRWIVEAFVDALGSVPGSGHDRAGDARGEIEDLLRADNVPPAVTLVTRPGV
                     GRTDSGRPTGPGVRPGRWSPYAGILDGGDPGELPDVRAGRVGVQDEGSQLVALAFARA
                     AVSGSDERWLDICAGPGGKAALLTGLARQRGATLVANERQPHRATLVQSALRAYGRPC
                     TVVCADGRTAPWRADTFDRVIVDAPCTGLGALRRRPEARWRRTPTDLELLVPLQRALL
                     RSALDSTRPGGVAAYVTCSPHPAETSGVVEAVLAERPDVSQEDARGLLPEVDLLGDGP
                     HVQLWPHRHGTDAMFLAVLRRLT"
     gene            complement(17628..18554)
                     /gene="fmt"
                     /locus_tag="VH419_14650"
     CDS             complement(17628..18554)
                     /gene="fmt"
                     /locus_tag="VH419_14650"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008356737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEY7044905.1"
                     /translation="MRVVFAGTPEVALASLRAVLESRHQVAAVVTRPDAPAGRGRRMT
                     PSPVATMAAEQGIETLKPDRAGDPDFLRRLKEIGPDCCPVVAYGALLPERALELSPRG
                     WINLHFSILPAWRGAAPVQRSLMAGDEVTGATTFRIVKDLDAGPTYGVFTEQIRPDDT
                     AGVLLDRLAEHGAGLLVRTLDGIEDGTLVEREQDETLASYAPKLTVEEAQVDWGRPAF
                     AVDRRVRGCTPSPGAWTTLEGQRVKVGPATPRPERTELGPGDLAVGKHTVLVGTGSHA
                     LELGEVQPEGKARMRAAAWARGLHVAPARFGT"
     gene            complement(18571..19116)
                     /gene="def"
                     /locus_tag="VH419_14655"
     CDS             complement(18571..19116)
                     /gene="def"
                     /locus_tag="VH419_14655"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012921905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEY7044906.1"
                     /translation="MSVRDIRLFGDPVLRTVASPVVDFDKEIRALVEDLTDTMLAAPG
                     AGLAAPQIGVGLRVFTYNVDDSVGHLINPELTLSEDTQTGEEGCLSIPGLTFDCTRAM
                     RAVARGFDMYGEPVLLEGTETLARCIQHETDHLDGILFVDRLDPATRKAAMKAIREAE
                     WNGMSAPIVKTSPHPTFGRGM"
     gene            complement(19174..21219)
                     /locus_tag="VH419_14660"
     CDS             complement(19174..21219)
                     /locus_tag="VH419_14660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017933569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEY7044907.1"
                     /translation="MSGPGEQLALLRTQVRGRRRLPVTGPAPTLPVARVAVDLPLPHL
                     DQTFDYLVPLALHEEAQAGCRVKVRFAGRYVDGFVVERAEETTHVGELAALRRVVSRE
                     PVLHAEVLTTARMVADRYAGTLADVLRLAVPPRHARVESEPTTPIETRLDLPVDALLT
                     GTPGQAGWSRHRGGEALLRRLADGQAPRAVWSALPGEDWPAVLAAAAAATASSGRGSL
                     LLAPDVRDVARLDAVLTSLLGAGRHVVLTADLGPAARYRAFLAVSRGHVPIAVGTRAA
                     AFAPVRDLGLVALWDDGDDLYAEPRAPYPHAREVLLLRAHHQQAAFLAASHARSVEAA
                     LLVATGWAAGISADRRTIRETAPQVHVTGETDHERERDVAADTARVPHRVFDVVRSAL
                     QLGPVLVHTPRYGYQPSLGCAGCRSPARCGRCRGPLARASAGGAAACRWCGEVAESWR
                     CERCGEDRFRAPVVGALRTAEEWGRAFPSVPVSSSGGGNVLGTVPDRPSLVVATPGAE
                     PPAEAGYAAAVLLDTWLSLGLPGFRAAEESLRRWFMIGSLVRPAAAGGRIIAVGDASA
                     PALQALVRWDPDGFADRELAQRESARLTPAARLATITGSAERVAEALVGLQLPRGAEL
                     LGPVPIGGADEPLVRVVVRTSRERGAGLSNALRQLQAARSSRKLPPVRVQVDPADLV"
     gene            complement(21274..21696)
                     /locus_tag="VH419_14665"
     CDS             complement(21274..21696)
                     /locus_tag="VH419_14665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009074562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEY7044908.1"
                     /translation="MPSRNRPARVDDVHELAAGMPHVTVYPGTDDNPVYQVGGKSFVF
                     FRNPRPDAFDPETKQRYADVIVIWVGSEADKEALVQDESSPFFTTPHFNGHRSVLVRA
                     SRLGELTVEELAEVIQDAWLARASPSRGKAWLQGQPSR"
BASE COUNT         3282 a         7777 c         7542 g         3199 t
ORIGIN      
        1 gacgaccgcg tgcatgccgt tggcgaaggt cgaacgcggc ggcgagtagt agtcggtgcc
       61 ttcgagcccc ggtgcttcac cacgctcgac cgggggcttg tccgggtcgg ccggcgcggc
      121 gaactgggtc gcccgggccg ccgcctcgtc gaaggcgtag cgcagcgggt tcgacagcac
      181 ggcgaccgca ccgaggtcga tctccgacgt ggggctgccc ttcacccgga cgtggccgtc
      241 gacgatctcc aggtcgtcga cggccgcctc caacgcgttg gccgcgatcc gcatcgcctt
      301 ctcccgcacc cggcgagcgg tcagcgcgac cgcgctcccg gacatcactg cagtgcgaga
      361 ggcgaacgtc ccgacggcgt acttgaatcg cctggtgtca cccgtcgtca cccggatccg
      421 cgcgatcggc accccgagct cgtcggacac gatctgcgcg agcatcgtct ggtgcccctg
      481 cccctgggtg gtcaggccgg tggagacgac gaccgtgccg tcggtctcga cccggatgtg
      541 gccaccttcg tacggcccga cgccggtccc ctcgacgtag caggccagtc cgatcccgac
      601 gcgacgtccc tcggcgcgtg ccttctcctg gtacgccggg aagtcgtccc agccgaccag
      661 cgccttgatc ttgcccagcg tggctgggaa gtcgccggag tcgtagatca gcggtcgccc
      721 gtcctggaag agcaagccgt ggtcgtacgg catctcgtcc ggcatgatga agttgcgctc
      781 ccgcaccacc gtccggtcca accctcgttc gtgggcgatg gcgtccatca cgcgctccat
      841 cacgaagcag ccctgcggcc gaccggcgcc gcggtacggc gtgaccagca ccgtgttggt
      901 gtagaggctc tggaactcca cccggtaggc gccgggcttg tacgggccga gcagctgcgt
      961 cgatgtgatg atcggcacga tcaggccgta cggcgtgtac gcaccgttgt cgtgccagaa
     1021 gcgcacggac aacccacgca gggttccgtc gtcgtcgtag ccgaactcga cctcgtgcag
     1081 ctgcccccgc tcgtgcgcgg ccgagacgaa gtgctccctg cggtcctcgg tccacttcac
     1141 cgggtggccg aggcggattg ccgcccacgg cacgaggacc tcctccggcc acggatgcac
     1201 gatcttcact ccgaacccac cacccacgtc cggagtgacg acctcgaccc ggtcgaccgg
     1261 caggctcagc ttggcggcaa tggcctgccg cacgctcgtg gacgtctgtg tcgacgactg
     1321 caccaggagg gactggtcgt tggcgtccca gcgggcgtac acgcccttgc cctccatcgg
     1381 catggacgcg ctgcgctcga tcgacagcga caggctgccc cggtgcggtg cggccgcgat
     1441 cgccgccgcc gcgtcaccga cctcctggat catcgtcgcc gccacgttgc cgggcacgtc
     1501 gtcgtggacc agcgcctcgc cgtcccgcgc gttctccaga ccgacgaccg gtggcaggaa
     1561 gtcgtagctg acgttcacga gtcccgcggc gtcctcggcg acgtaccggt cggtggcgac
     1621 gaccatcacg atcgcctcgc ccacatggtt cacctcgtcc ttggcgagcg cgtacggcgt
     1681 ccggccctgc gtcagcgtcg gatgcgggat cagcagcggc aacggctcgg cggtccgtcc
     1741 gtccaggtcc tcgtgggtgt agatcgccac caggccgtcg acgtcgaggg ctgcggacac
     1801 gtcgatgtcg acgatccgcg catgtgcgtg cgggctacgg acgaacgccg ccgccagcgc
     1861 gtcgtggccg aggtcgtcga ggtaggtacc gcgaccggac agcagtcgcg ggtcctcgac
     1921 gcgcgggatg cgctgaccga acatcttcgt ggtcaccggg ccggctctcc cgttgccgtc
     1981 tcaccggtgg cctgatgccg gatctcggca gcgcgcagca ccgccgccac gatgttctgg
     2041 tagccggtgc agcggcagag gttcccgccg atcgcctccc gtgcctcctc ctcggttgga
     2101 tccggattcg tctccaggta tgccgtgatc gtcgtgatga agcccggagt acagaagccg
     2161 cactgcagcg catggcagtc ctggaaggct tgctgaacag gcccgagtcc gccgttcggg
     2221 agcagacaac cctccgtcgt ggtgatacgg ctcccgtccg ccgtgaccgc gagcacgagg
     2281 cagctgcgta cgggcactcc gtcgaggagc accgtgcacg cgccgcacac cccgtgctca
     2341 cagccgacat gcgttccggt gagccccgcg tcgtgacgga tcgcgtccga gagcagccgc
     2401 cgcgccggca cggccagcgg gtagagcacg tcgttgatca ccagcgtgat cgcatgcagc
     2461 tgctcgctca tgtgccgccc ccgatcgtcc ggccgcgggc cgcggccgcc gccttggtca
     2521 gcgcgcggcg gctgagcacc cgcgcgagct cccggcggta gtcggctgag gcgtggatgt
     2581 cggtctcggg gtcgatcgcc tggtcgacca gatcgaccgc gtccgcccag tccgactcgt
     2641 ccgcagtgtc accggtgacc gcactggtca ggtcgacgac gacgggtacg gggccgacgc
     2701 tgacgagact caccgacgct gccgcgacca cacctgcctc gagccgcacc atggcgccga
     2761 cgccaaccat cgcgaagtcg ccccgtcgcc gcgacaactc gagccaggcc gtcccggccc
     2821 cttccggagg atccgggaag tacgccgaga cggccatctc tgcgtcggtc agcgccgact
     2881 cgagcggacc gacgaagaag tcgctcgcct cgatccgacg ccgcccgcgc ccgcggctga
     2941 ccgcctcgac gtatccaccg agcagtgcga gcacggccgg catctcggct gcggggtcgg
     3001 cgtgcacgag gctgccgacg gtcgtaccgc ggttgcggat ggtggcgtgg gccacgtggc
     3061 tgagggcgtc acgcaggagt gggttggcgt cgtacgccgc tcggtcgtgc tcgagcccgg
     3121 catgccgcac gagtgcgccg acgcggacac ccccgtcgcc gacctcgatc gtgtcgagcc
     3181 ggtcgatgtg attgatgtcg acgagcgctg gcggcgcggc cagtcgcatc gacaggatcg
     3241 ggaccaggct ctgcccgccg gccagcacct tgcccccggt ggcggccagc gcttcgacgg
     3301 catcgtcgac actggccgct cggtgatagt cgaacggcgc cggcttcacg gtcggaaacc
     3361 tccggttggg cgggcagggc tcgctgcgtc acgagtcggg gtcgcggcgg tggctccccc
     3421 ccggctcctc ctcgacctcg tgcaggcctg attcacctac ggagatcatc gtgcctggat
     3481 cgtcgacgcg caggtgagcc ggggcgatcg cccgggccag gtggtagaag acgatgacga
     3541 caatcgtgcc gagcgcgatg ccggacaggg tgaagtcgtc cgtgatctcc aggctcgtgt
     3601 cgccgatggc gatgatgatg cccgccgcca acgggaccag gttgacgggg ttgcccaggt
     3661 cgacgttgtt ctccttccag atcttcgcgc cgagcaggcc gatcatgccg tagaggacaa
     3721 cggtgatgcc gcccaggaca ccacctggtg tcgacgacac gagtgctccg aacttcgggc
     3781 tcaggccgaa gaggatcgcc accacggctg ccacgtagta cgccgccgtc gagtagaccc
     3841 gggtggctgc catgacgccg atgttctcgg cgtacgtcgt ggtcggcgaa ccgccgacgg
     3901 acgaggcgag tgcggtgccg aacccgtcgg cagcgatcgc cttgcccatc accgggtcga
     3961 ggtcacggcg tgtcatctcg gcgaccgcct tgacgtgtcc ggtgttctct gcgatcagcg
     4021 cgatcacggc aggcaacacg agcagcgtga acgtgagcga gaagcttggc cagtgccagc
     4081 ccacgacacc gttctcggtg tgcggcggga aaccgatcca gtccgcagac ttgaccgcat
     4141 cccagctgat ccggtcgtgg tcgaacggcg aggccacgca gtagaggccc tctggcgagc
     4201 acggcttgcc gtcggggccg agcaggttct gcccagggag cacgctgttg acctggctga
     4261 acgagtcggc gatccacgag atcacgtagc cgaagatcaa cccgacgaag atcgcgatgc
     4321 ggccgaggaa gcctctgaca ccgacggcga agacgatgac gaacgccatc gtgatcagcg
     4381 cgatccactg gtcctgcggc cagtagatgt cggcgaccac cggggccagg ttgaacccga
     4441 tgagcatcac gacagcgccg gtgaccgccg gtggcagcac ggcgtgcagg acgtgcgagc
     4501 cggcgtagtg gatcagcaca ccgatcagca gcagcaccag gcccgccacc aggatcgcgc
     4561 cggtgacgtc agccgagtcg ccaccctgcg cgcggatggc cgcgacaccg ccgacgaagg
     4621 acgcgctcgt gccgagatag ctcggcacct tgccggagac gatgagcagg aagcagatgg
     4681 tggcgattcc gctcatcatg atggcgagct gcggattgag ccccatcacg atggggaaca
     4741 cgaacgtcgc gccgaacatc gccacgacgt gctgggcgcc gagcccgact gtcctcggcc
     4801 acgagagccg ctcgtccggt gctacggcct cccccggcgg cggcgtacga ccaccgtgca
     4861 cgactgtcca cccaaaagcc atggcccact cccgaatcaa tcaggtgtga cgtgtgtgtc
     4921 acccaactcc accatgccgg aaacgcactc cgggatacac gcgacgcgca ggccacgaca
     4981 cagcggctcc gggcggggag tgtcggcatc ggccgggttg acggggaggc tcgcttcgtt
     5041 ggtggcgcga gccagggact ctcaggtatt ccgtcggcgg catcggtcgt cgatgggtgg
     5101 ccgtgaccgc ccgggccagg tcgcctcggc tgaggtgggt cggcagcgaa gcgccggagt
     5161 cgttcgtgcc cgatgagccg atcggccgac gttcggttcc ttgtgcctgg tgtgcgactg
     5221 gccggttccg gacatccgcc gttgaccgtc ctcgtgtttg cgatgtccac atccgagacc
     5281 ccctgtgccc atcggaggtt tgcgatgttt acacgccgct tgggctgtaa acatcgcaaa
     5341 ccaccaccga cacatctctg actgcacctc gggtggcccg aaggcaggct cgtgtgacaa
     5401 ctcgcgtgcg tcccccgtgg gccgttggca cgtctgcgca tgtgagagag accgtggcgc
     5461 agcggctagc gtgtgtctgg agagaaagga agttgcggca gcgtggtgcg aaatgtgccg
     5521 ggacgcacgt ggcgggcgag gctgagacgt ccgtcacgtc cgatggcagc aagcatcctc
     5581 gttgcccccg tgatcgacgg tccacaccgc cacctgcacg tggttcatcg ctcccggttc
     5641 gctctccagc tcgctgacga gacaggtcgt gtcgatttcg ccgtcacgtt tcccggggcc
     5701 gtgcgtctcc cttatgcatg cgcggtcgct tcaccggtca gtgcctcgtc atcgttctcg
     5761 gtcggagacg gtgtcctggc ctggggcgac gatctgtgca acacgtgcac cacgccctcg
     5821 acgacgtggc ccggcgtcgc ccccgctgga ccgacgagct ccctggtgac gttcacggtc
     5881 gcgcgttggt ggcggccggc gaagccccgc caccacgcgc tggccgaggc tgtcgacccg
     5941 gccggcgtac gtcggctggt cgacacctgg cccgatctgc ttggccgggg aaccggcctc
     6001 acaccgtacg gcgacgacgt cctctgcggc gcactcgtca cattgtcggc agtgcagcat
     6061 cccgatttcg cgcccctggc cagtgacatc tccgcggcct ctctcgacga gcgcaccacg
     6121 gcgacatcgg cggcgctgct gcgggctgcc tgcgaagggt ggtgcatcga cgagctcgca
     6181 tggcatcttg ccgcgctggc gacgccggac ccggcgtcat acgccgcggc cagccggcgc
     6241 cggctccacg cggtcggctc gtcgtcgggc cgcggcctgc tcgaaggcgt cggcctggtg
     6301 ctcggtgacc gtgctgggag ggtcgccgcg tgacacgcgc cgtcgagatc cgtcgcggtg
     6361 cctaccagga ctccgtcagc ctcatgcagc tcagccggga gctcaccgga cggcccgacg
     6421 tgaccaacgt ccaggtcgcg atggcgaccg agctcaacct cgagctgctg gagcagatgg
     6481 gcttcgaggt gccggccgag gcgacgccca acgacatggt ggtggccatc gaggcgcctg
     6541 acgacgaggc gatcgcggcg acgcgaagcg ccctcgacga ggcgctgacc agaacgccgg
     6601 cccgcaccga cgggtgggac gacgccacgc cgccggctcc cacgctcggc gcggcggctc
     6661 gacgggttga tgcgtcgctg gccctggtga gcacccctgg ccgggtcgcc gtggtcgatg
     6721 ccgcggacgc actggccgcc ggtctggacg tgatggtgtt cagcgacaac gtctcgctcg
     6781 accacgaggt cgcgctgaag gactgggccg ccgaactggg gctgctcgtg atgggtcccg
     6841 actgtggcac ggccgtggtc gacggggtcg ggctcgggtt cgcgaacgtc gtacgaccgg
     6901 gtccggtcgg catcgtcgcc gcgtcgggga ccggcgcgca acagctcctt gcgttgctcg
     6961 acggcgccga cgtgggcgtc aagcactgtc tgggcgtggg cggccgcgat ctgtcggccc
     7021 ccgtcgcagg ccgctcgacg ctcgcggcac tggaccggct cgcagccgac gacgatgtcg
     7081 agacgatcgt ggtcatctcc aagccgccgg cacctgacgt cgcggcccgc gtgacagaac
     7141 acgcgaaggc gttcgacaag ccggcagtca tcggctatct cgggcctggc cagcccgacc
     7201 tgaccacgac cgcgcaacgg gtcgccgaag ccgcaggagc agcctggaca ccgccgcgac
     7261 gctggggacc ccagagtcca ccgcctcgcg atggtttcct gcgggggttg ttcagcggcg
     7321 ggaccctgtg cgacgagtcc atggtgctcg ccgtcgaaca ccttgggaga gtcgcgtcga
     7381 acatcccgct ggacggacag tccacgctgg ggcacgacct tgccggcgcg ggccacacct
     7441 gtatcgactt cggcgacgac cagctcaccg ccggacgccc gcacccgatg atcgacccga
     7501 gtctgcgact ggaccggttg gagcgcgagc tcgcggatcc cgagtgtgct gtcgtgctgc
     7561 tcgacgtcgt gctcgggcac ggtgcgcatc ctgacccggc gaccgacctg tccgacgtca
     7621 tcgccggctc cacgacgccg gtcgtggtct ccctcgtcgg cacccgcgac gacccgcagg
     7681 gtctggaagc acaggcgacc cgactcgcag aggcgggcgc cgtggtccat gcatccaacg
     7741 cagccgccac ccgggaggcg ctggccatca tcgaagggcc aggccaatga gctcggaccc
     7801 gatctcggct gccgcggtcg tcaccgccgg cgcggcgttg ctggaggaga gcctgcgggg
     7861 acagggcgtg accgtcgagc ctgtcgcgtg gacgccgccg gtcgccggga ccgagaccgc
     7921 gctagcggcc gtgatgctcg accgcaggcg ggccgccgcc aacaccgagg cggtacgacg
     7981 gatgacgtcc gcccgcgcca ccctggtcga cgtacggccc gccgcgactg cgctcggact
     8041 cgaacgtggt gagtttctgc acgcgggacc gcccatcagc tgggaccgcg cctccggtcc
     8101 actgcgtggg gccctcatcg gcgccgtgct cttcgaggga ctcgcggcga cggccgagga
     8161 agcggaggcg ctgctgtcgt ccggtgcctg tcgcctcgag ccgtgtcacc atcgtggcgc
     8221 ggtcggcccg atggccggtg tcgtgtcacc gtcgatgtgg gtgttcgagc tgcgcgacga
     8281 ggtgcacgaa gcgacgtcct ggtgttcgct caacgagggc ctcggcaagg tgctgcgcta
     8341 cggcgcctac tcccccgacg tgatcgaccg gctgcactgg atgaccgacg tgctcgggcc
     8401 cgcgctcggc gccgccaccc gggcgcgcgg cccgatcgac atcaccgcga tcatcgccca
     8461 gatggtgcag atgggcgacg agggccacaa ccgcaaccgg gccgggacct tgatgctgct
     8521 caaggagtgg ctgccggacc tgatcacgtc ggggttgccg acggccgacc tcgccgaggt
     8581 cgtcaggttc gtcgccggaa acgaccactt cttcctcaac ctcgtcatgc ccgcctgcaa
     8641 gctcatgacg gtggcggcct cgggcatccc cggttcgagc gtcgtcacca cgatggcgcg
     8701 caacggcacg gacttcggca tccgggtctc cggtaccggt gaccggtggt acgtcggccc
     8761 ggctcagctc gccgacggcc tgtacctcgg gaactacggg ccggacgacg ccaaccccga
     8821 catcggggac tcggccatca ccgagacggc cggcatcggg gggttcgcga tggcgacggc
     8881 gccggccatc gttcggttcg tcggcggctc cgtccccgac gcgctggcca cgacgaggcg
     8941 catgtacgac ctgtgtgtcg gcgagaaccc cggctgggcg atcccgatca tggactttcg
     9001 cggtacgccg accgggatcg acgtcacgtc cgtcctgcgg tccggtcagc tcccccagat
     9061 caacaccggc atggccggca aggtcgccgg caccggccag gtcggcgccg gtctcgtcac
     9121 gccgccgatg gagtgcttcg tcgccgccat cgaaggcctc gccgaggtgg caacggcttc
     9181 acgacacgcc gacaaactcc aaccaaacct gccacctcgg cgttgaacta ccgcgttggg
     9241 cctggcgttg aactcgccgc ttgcggggtc aggtgtcgtg atcggtgggg gtgtggcggg
     9301 cgatgacgcg ggccagctcc ttcgccgaac ggatggcccg tgctccgtca gacgtctgga
     9361 tcgccatcac cgcctgcgtc gtaccgtcgt cgaggtccat cagcgcccac ggacggttcg
     9421 gtgacagcga gatccgcacg atctgacccc actcgaggaa ccgccgtcgg tagccgttga
     9481 cgacctccag gccggattcg gtggcgcgtg cggaggtccg gaagatcgcg ttgagcagca
     9541 cgaggatcgc gacgaagaac gcgaccaggg tgatgcgctg aaaggtcgtg aagtcgtcct
     9601 gcacgttgcc cggaagcatc agccacagga acgtcatcgc caccacgatg gccactgctg
     9661 ccgtggcggt ggcgacgcgg gcgccgagcg gacggtaggt acgcggcagg tgcacctcgt
     9721 agggtgtcgc cccatcgcgc gtggcgtcag agtcggcagg catggatgtc ggtgacgagg
     9781 atgccgcgcg ccccgagctc gtacagctcg tccatcaccc gctgggcgtc gtcacgcggg
     9841 accatggaac gtacggcgac ccagccgtcg cggtgcaacg gcgccaccgt cggcgactcg
     9901 ataccagggg tgagggcaca cgcggcctcg acgtcctcga cgccgcagtc gtagtccatc
     9961 atcacgtacc tacgtgcgac caggacgctc tggatgcgcc ggaggaagac ctcgaacccg
    10021 ttgggatcgg gggtgttgtc ccgcgtcacc agcacagtct cagaggtgag gatgggctcc
    10081 ccgaagaact cgagtcctgc ctgccggacg gtgttgccgg tggacacgac atcggcgatc
    10141 gcgtcggcca cgcccaggcg gaccgcggac tccacggcgc cgtcgagccg gaccaccttg
    10201 gcgttgacgg cacgttcctt gaaccagtcc tcgaggattc cggcgtacga cgtcgcgacg
    10261 cgggccccgt cgagatcgtc agcgcccgcg aacgtgccca ccggggcggc gaaacggaag
    10321 gtcgactcgc cgaagccaag cgtgaggcgc tcgaccacgg gcgcgcgcga gtcgaggacg
    10381 agatcccggc cggtgagacc gacgtccagc gtcccctcgc cgacgtacac ggcgatgtcg
    10441 cgcgggcgca ggtagaagaa ctcgacgtcg ttcatgtcgt cgctcatcac caggtcgcgc
    10501 tcgtcgcgac gctgccggta gccggcctcg cggagcatct gggtggcggg gtcggcgagc
    10561 gagcccttgt tcggtacggc gatcttcagc atggcggcgg tcctacaggt gcgcgtagac
    10621 gtcgtccggg gtgagcccgc aggcgagcat catgacctgg gcctggtaca gcagctggct
    10681 gagctcctcg gccgtccgct ccttcgactc gtgctcggcc gccatccacg actcggccgc
    10741 ctcctcgacg agcttcttgc cgatcgcgtg gacgccggcg tcgacggcga cgaccgtgcc
    10801 cgagcccggt gttcgctcgg ccaccttcgt cgccagctcg gcgtacaacg tgtcgaaggt
    10861 cttcatcggc caccagccta atggcgctgc ccctgacgct tgcgtccgtg gagctgctcg
    10921 tcgagacagc cggcgctacg gacgcaaggc cgccagcgtg agggcggtcg acagggcggc
    10981 atgggtggcg tcgtacccct tgtcctcctt cgagccctcc agtcctgccc ggtcgagcgc
    11041 ctgctcctcg gtgtcacacg tgaggacgcc gaacccgacg ggtacgccgg tgtcgagcgc
    11101 cacccgggtg agccccgcgg tcacggcatc gcagacgtag tcgaagtgcg gagtgcctcc
    11161 ccgtacgacg acgccgagcg ccacgaccgc gtcggcgccg tcggaggcga cccgctggga
    11221 gacgatcggc agctcgaagc tccccggcac ccggacaacc cggtactccg ccacgttcgc
    11281 ctctgacagc gcacgctcgg cgccggcgat caacccgtcc atcaccgtgg catgccactc
    11341 ggcggcgacc acgacgaccc gcagctgggc ggcgtcgatc gacgtgaggg tcggctgtcc
    11401 ggttccactc atgctggcgg tcctccgatt cggttctcgc tgctgtccac ctcgtcgtcg
    11461 gcggctgtcg agacgggccc gaggtcgagg tggctgagct cgtgtcccat ccggtcgcgc
    11521 ttggtcgcga ggtagcgccg gttctcctcg tgcgtcatac ccgggacggc gatccgctcg
    11581 gcgaccgtga cgccgaaccc ctccaacgcc gtgcgcttgt ccgggttgtt cgtgagcagc
    11641 cggaccgagg tgatgccgag ctcgtgcagg atctgcgcgc ctgtgccgta gtcgcgcgca
    11701 tcggccggaa gccccaggtc gaggttggcg tcgacggtgt cgaggccgtc gtcctgcagg
    11761 gtgtacgcct tcagcttgtg gagcagtccg atgccgcgtc cttcgtgacc acgcaggtag
    11821 acgaccacac cacggccctc ccggctcacc gcctccagac acgcgtgcag ctgcggaccg
    11881 cagtcgcacc gctgggacgc gaagacgtcg ccggtgaggc attcggagtg cacccggacg
    11941 agtacgtcgc gaccgtcgct gatatcaccc ttgacgagcg cgatctgctc ggacccgtcc
    12001 acgaggttgc ggtagccgag ggcctggaac gtgccgaact cggtgggcag ccgcgtctcg
    12061 gcgacccgct ggacctgccg ttcgtgccgc cgccggtagc ggatgaggtc ggcgatcgag
    12121 atgagcgcga gcccgtgctc gtcggcgaac tcgcgcagct gcgtgccgcg cttcatcgtg
    12181 ccgtcgtcgt tgaccagctc ggagatcgca ccggccggcg tgagccccgc aagccgggcg
    12241 aggtcgaccg aggcctccgt gtggccgggc cggacgagta cgccgccgtc gcggtagcgc
    12301 agcgggaaga cgtgtcccgg ctgcacgacc tcgaacggct ccgtcgccga gtcgaccagc
    12361 acccggatcg tgtgggcacg gtcggcggcc gagatcccgg tggtcacacc gtcacgggcg
    12421 tcgacggaga tcgtgtacgc cgtgcgcatc cgctcccggt tgtgcggcgt catcaacggg
    12481 atcgccaacc ggtcgagcgc cggaccctcc atcggcacac agatcacgcc gctggagtac
    12541 ctgatcatga acgccagcag tttcgtggtc gccttgctgg cggcgaagat caggtcgcct
    12601 tcgttctccc ggtgctcgtc gtccacgacg acgaccggct tgcccgccgc aacgtccgtg
    12661 atcgcgcgct cgatggcgtc cagccgcacg gacggtctcg tcgcggcagc tgcacgtgcc
    12721 gcggaagggg tcgaaggcgt tgagaacgtc ataagccaac catctctgct gtcgggtcgg
    12781 ccagccgctc gttccgtgcg atccgccgcc aggagaagaa cccgtagatc accagcaccc
    12841 cgtaggcggc gtacagcatc gccgacgggt agagctggaa gtgaatcagc tcgggtacgc
    12901 cgaccaggtc caccgcgagc cagcacaacc agaagtcgac ccagccacgc gccatggcgt
    12961 acgtcgccag gatcgagccg acgaagatcc aggcgtcggc cacgtagtac cagctcggcg
    13021 ccggccagcc cgccccgatc cagcggaaca cgtacgtcag cacgaggacc gcgaccgccg
    13081 cggccgcgag ataggccgcc cgctcacgcc cggtcgccca gcgcgggatg acggccggtc
    13141 cgtgctgggc cgatcggtgc cgagtctggc gccaccgatg ccagccgtag acgctgacga
    13201 tgatgaagaa gatctgccgt ccggcctggc ccagcagcgg gatgtgctgg tccccgacga
    13261 cggccgtccc catgaacacc gtgaacagca agacgttgcc gatgatgccc accggccacg
    13321 cccacactcg tcgcttgagc ccaccgatgg cggaggcgaa accgaacgcg ttgccgatga
    13381 tctcgcgcca ggtgatctcg tgtccgctga tcgtcaggtg ggcgtcgtac gcggtctgga
    13441 agaggctcac gggcgctcct tcgtcgacgc gccgtctggt acgtcgagca gccgctcgac
    13501 atacttcgcg atcacgtcga cctcgaggtt gaccgggtcg ccgggctgtt tgaacccgag
    13561 cgtggtgagt tcgagggtgg tggggatcag gctcacctgg aaggccggct ccggctcgtc
    13621 gaccaggccg ctgacggtca gagagacgcc gtcaacggtg atcgagccct tcatcgcgac
    13681 gtaccgcgcg aggtcggccg gcagtgcgat cgtgaccacg tcccagtgct cgctgggctc
    13741 gcgacgcacg atcgtaccaa cgccgtcgac gtggcccgaa acgatgtgac cgccgagtcg
    13801 gtcctgcggg ccgacggcgc gctcgaggtt gacccggctg ccctgcgtca acgctccgag
    13861 ggaggtccgg tcgagacttt ctgccatcac atcgaccgtg aaccgctcgt cgtcccactc
    13921 cacgacggtc aggcagcagc cgttgaccgc gatcgagtca ccgtggccgg cgtcgctgac
    13981 gacacgcgga ccacgcacgg tcagccggat cgagtccccg agctgatcga gcgcgacgac
    14041 ctcgccgagc tcctcgatga tgcccgtgaa catcagcggt cctcctccgc gggtcgcgtc
    14101 ggggaccgca tggtgaggcg tacgtcgtcg ccgacctgga cgacctcgag cagcccgaag
    14161 cgccgaatgc cgcggatcga cgtgacgccg aggtcaccca cagcagacag ccccgcgccg
    14221 agcagcgccg gggagacgta ggcgatcagc tcgtccacca gaccggcccg cacgaacgcc
    14281 gccgccaacg tcggcccgcc ctcgaggaag acgtgctggc ggtcgtcggc gtacagcttg
    14341 gccagcgcca cctgggggtc ccgtgtcgcc aggaacgtcg tcggggcggt ggcgtcacgc
    14401 acccgggcgc cgtcagcgat cggccgcagc cccatgaccg cacgcagcgg ctggtgctcg
    14461 accggaagcg gatcgtcgtg ctcgtcgcgg acgttgagct gagggtcgtc ggcgagcacc
    14521 gtcccggtcc cgaccaggac cgtgtcacac agggcgcgct ggcggtggac gtctgcacga
    14581 gccggcccgc tcgtgatcca gcgggcggac ccgtcggcgg cggccgaccg gccgtcgagc
    14641 gtggccgcga gcttccaggt cacgaatggt cgctggtttc ggacagcgaa cgaccagatc
    14701 tcgttgatgg cctccgcctc ggcggcgaga acgcctcctt cgacgtcgat gccggcgtgt
    14761 cgaaggacct cggcgcctcc gtcggcgatc gggttgaggt cgtgctgcgc gaagaccacc
    14821 cggcgtacgc ctgctgcgat cagggcttgc gtgcacggcc cggtccgacc ggtgtggttg
    14881 cacggttcca ggctgacgac ggcggtcccg ccggccgcaa gctcgccggc cgcctgtaac
    14941 gccgccactt ccgcgtgcgg cgacccgacg ccacggtggt agccctcgcc caccacccgg
    15001 cctccagcat cgagcacgac ggcgccgaca cgtgggttgg gaccggtcgg tacgccaggc
    15061 gtgcacgcca acgcgaacgc ctgggtcatc gccaggactt cgctgtcggt tgccacgccg
    15121 cgctcctgtc gccgtctcgc ctcacgggat ccggggtggc gacgacgcga cgcctgccgt
    15181 ccggcgaggc ggtccggcgt actcacgccg ctgactcgcc tgtcccgccg ccggcgggcg
    15241 acgctgcgtg catcctccca tccggacttt caccgtcggt cccggagttc caccgggtca
    15301 accggccagg atcggccggg tcgcggactg tcaccgccgg ttcggacttt caccgacccc
    15361 ggagcacgcg agagctggtc tcttcgccgt ttcagtatga caccgacgtc tgagcgcggg
    15421 tacgcaggct cttcaccgcc gcatcggggt cgtcggaacc gaagacggct gatccggcca
    15481 cgaagaagtc cgcgcctgcc tctgcacatc gctcgatcgt gtctgcggcg accccaccgt
    15541 cgatctggag ccagacctct ccgccatacc ggtcgatcat ctcgcgggca cgccggatct
    15601 tcggcaggca cccgtcgagg aactgctggc caccgaaccc gggctccacc gtcatgatca
    15661 gcagcaggtc cagctcgggg agcatggctt cgtacggctc gaccggcgtc gccggcttga
    15721 gcgccatccc ggcccgggcc tgcatcgcgc ggagctctcg ggccagccgc accggtgcgg
    15781 cacacgcctc aacgtggaac gtcacgctcg acgcgccgac ctcgacgtac gccggcgccc
    15841 agcgatccgg gtcctcgatc atcaggtggc agtcgagcgg ctgctcggcc accttcgcca
    15901 acgcctcgac gaccggaggg ccgatcgtca ggttgggcac gaagtggttg tccatcacgt
    15961 ccacgtgcag ccagtcggcg cccggcaccg atcgcgcggc cgcctcgaga ttggcgtagt
    16021 cggcggacag gatgctcggc gcgatcagcg gcgtggtcag cttggccatc ggtcgagcag
    16081 tctatgccgg gcccgccccc accgccgagg tggcaacttt ggttgcgttc taccggcgtg
    16141 tcgaaccgcg agtgccacct cggtatcgcg ctgatcgagc tgcgcgatcg gtacgtccat
    16201 cgcgtatcag gtgagtcggc ggaggacggc gaggaacatg gcgtcggtgc cgtggcggtg
    16261 cggccagagc tggacgtgcg gtccgtcgcc gagcaggtcg acctccggga gcagacctcg
    16321 cgcgtcctcc tgcgagacgt ccggccgctc cgcgaggaca gcctcgacca cgccggacgt
    16381 ctccgccggg tgaggcgagc acgtcacgta cgccgccacc cctccgggcc gcgtcgagtc
    16441 cagggcagag cgaagcagtg ctcgctgcag cggcacgagc agctcgaggt cggtcggtgt
    16501 acgccgccag cgcgcctccg gacgtcgacg cagcgccccg agccccgtgc agggtgcatc
    16561 gacgatcacc cgatcgaacg tatccgcccg ccacggagcc gtccgcccgt cggcacagac
    16621 gaccgtgcac ggtctcccgt aggcgcggag tgcggactgc accagggtcg cgcggtgcgg
    16681 ctgtcgttcg ttcgcgacca gcgtcgcgcc gcgctgccgg gccagcccgg tgagcagggc
    16741 agccttgcca cccgggccgg cacagatgtc gagccagcgc tcgtccgacc cgctcaccgc
    16801 cgcccgagcg aaggccagtg cgaccagctg cgaaccctcg tcctgcactc cgacgcgtcc
    16861 ggcgcgcacg tccggcagct cgcccgggtc accgccgtcg aggatgccgg cgtacggcga
    16921 ccagcgtcca ggccggacac cggggccggt cggccgaccg gaatcggttc gaccaacacc
    16981 tggccgggtc accagcgtca ccgccggcgg cacgttgtcg gcgcggagca ggtcctcgat
    17041 ctcgccgcgg gcatccccag cacgatcgtg gccggatccc ggcacgctgc cgagggcgtc
    17101 gacgaaggcc tcgacgatcc agcgcgggtg cgcgtgcacg gcggcgaggt ggccgacgag
    17161 atccgcgtcc cgcgccggtg ccagcgcgct gagccagctc cccaggtcgc ggtcggcaac
    17221 ccggcggagt acggcgttca ccagccgtac cggccgttca ccgacctccg tccgggccag
    17281 ctcgacggtc gtgcccaccg ccgcatgggc ggggactcgc atgctcagca gctggtggac
    17341 gcccagccgt agcgccgtca gcacggccgg ttgcaacccg gcgacgccac cggtcgccag
    17401 cgcgtccagg atcaggtcgt agctgccctg gaggcgggcc gtcccggccg ccagctccgt
    17461 cgcgaaggcg gcgtcccgac ctgcgatccg ccgctgcgag agcaggcggg aaagcgtcag
    17521 attgaggtac gcgtcgtcga tctcgaccgc ccgcaacgtg tcgtaggcca cctgtcgggc
    17581 cgggtcgacc ggcgtcctcg cgcgggcccg ccgcggcgcg tcaggcatca ggtgccgaag
    17641 cgggccggtg cgacgtgcag gccgcgcgcc caggcggctg ctcgcatccg ggccttcccc
    17701 tcgggctgca cctcgccgag ctcgagggcg tggctgccgg taccgacgag gaccgtgtgc
    17761 ttgccgaccg ccagatctcc cggtccgagc tcggtccgct ccgggcgtgg cgtggccggc
    17821 ccgaccttga cgcgttgacc ctccagcgtc gtccaggcgc ccggcgacgg cgtacacccg
    17881 cgcacgcgcc ggtcgacggc gaacgccgga cgcccccagt cgacctgagc ctcctcgacg
    17941 gtcagcttcg gggcgtacga cgccagggtc tcgtcctgct cacgctccac gagggtgcca
    18001 tcctcgatgc cgtccagcgt cctgacgagc aacccggccc cgtgctcggc cagccggtcc
    18061 agcaggacgc ccgcggtgtc gtccgggcgg atctgttcgg tgaacacgcc atacgtgggg
    18121 ccggcgtcga ggtccttgac gatgcggaac gtcgtcgctc cggtcacctc gtcaccggcc
    18181 atcagcgacc gttggacggg ggcggcgcca cgccaggccg gcaggatcga gaagtgcagg
    18241 ttgatccagc cgcgcggcga cagctcgagg gcacgctccg gcagcagggc cccgtacgcg
    18301 acgacggggc agcagtccgg cccgatctcc ttcagccgac gcaggaagtc agggtcgccg
    18361 gccctgtcgg gcttgagcgt ctcgatcccc tgctcggccg ccatcgtggc gacgggcgac
    18421 ggcgtcatcc ggcggccccg cccggcgggc gcatcggggc gggtcaccac ggcggccacc
    18481 tgatgtcgcg actccaacac ggcgcgcaac gacgccagcg ccacctccgg cgtaccggcg
    18541 aaaaccaccc tcacccgcag aaccgccgca ctacatcccg cgcccgaacg tcgggtgggg
    18601 actcgtcttg acgatgggcg ctgacatgcc gttccactcc gcctcgcgga tcgccttcat
    18661 ggccgctttg cgggtggccg ggtccagccg gtcgacgaac agaatgccgt cgaggtggtc
    18721 ggtctcgtgc tgaatacacc gggcgagcgt ctccgttccc tcgagcagca ccggttcgcc
    18781 gtacatgtcg aagccgcgag cgaccgcacg catcgcgcgc gtgcagtcga acgtgagacc
    18841 cgggatggaa agacatccct cctcacccgt ctgcgtgtct tcgctgaggg tgagctcggg
    18901 attgatgagg tgaccgacgg agtcgtcgac gttgtaggtg aacacccgga ggccgacgcc
    18961 gatctgtggt gcggcgagac ccgcgccggg cgcggccagc atcgtgtccg tgaggtcctc
    19021 gacgagcgca cggatctcct tgtcgaagtc caccaccggc gatgccaccg tacgcagcac
    19081 ggggtcaccg aacagccgga tgtcgcgaac ggacaagggc actccagacc tcaggccaga
    19141 ccactggcgg aacggcggta agtctaggcc ggatcagacc aggtcggcgg ggtcgacctg
    19201 tacgcgcacg ggcggcagct tgcgactgct gcgtgccgcc tggagctgcc gcagcgcgtt
    19261 cgagaggccg gcgccgcgct cgcgggacgt gcggaccacg acgcggacga gcggttcgtc
    19321 ggcgccaccg atcgggacag gacccagcag ctcggcgccg cggggcagtt ggaggccgac
    19381 cagcgcctcg gcgacccgct cggccgatcc cgtgatcgtg gccagccgcg ccgccggcgt
    19441 gagccgcgcc gactcccgct gggccagctc acggtcggcg aagccgtcgg ggtcccaccg
    19501 gaccagtgcc tgcaaggcgg gcgccgacgc atcgccaacc gcgatgatcc ggccaccggc
    19561 agcagcgggc cggaccaggc tcccgatcat gaaccaccgc cggagagact cctccgcggc
    19621 gcggaaaccg ggcaggccga gcgagagcca ggtgtcgagc aggacggccg cggcataccc
    19681 cgcctccgcc ggcggctccg ctcccggcgt ggccacgacc aggctcggcc ggtctggaac
    19741 ggtgccgagg acgttgccac caccggagct gctcaccggg accgagggaa acgcgcggcc
    19801 ccattcctcg gccgtccgca gcgcgccgac cacgggtgcc cggaaccggt cttcaccgca
    19861 ccgctcgcac cgccaggact cggcgacctc gccgcaccaa cgacaggccg cagcgccacc
    19921 cgctgacgcg cgtgccagtg gaccgcggca acgaccgcag cgggcgggtg accggcagcc
    19981 cgcacagccg agcgaaggct ggtagccgta gcggggtgtg tgcacgagca ccggaccgag
    20041 ctggagcgcc gaccgaacga cgtcgaagac ccggtgcggc actcgggccg tgtctgcggc
    20101 cacgtcgcgc tcccgctcgt ggtcggtctc gccggtgacg tgcacctgtg gcgccgtctc
    20161 ccggatcgtc cgacggtcgg cgctgatgcc ggctgcccaa cccgtcgcga ccagcagcgc
    20221 agcctcgacc gaacgcgcgt ggcttgcggc caggaaggca gcctgctggt ggtgggcacg
    20281 cagcagcagg acctcgcgag cgtgcgggta cggcgcccgg ggttcggcgt agaggtcgtc
    20341 accgtcgtcc cacagtgcga ccagacccag gtcacgcacc ggggcgaagg cggccgcccg
    20401 ggtgcccacc gcgatcggaa cgtgacctcg cgacaccgcg aggaaggccc ggtaccgggc
    20461 ggccggaccc aggtcggcgg tgagcacgac gtgccggccg gctcccagca acgaggtcag
    20521 cacagcgtcg agccgagcca cgtcgcgcac gtccggggcc agcaagagac tcccccggcc
    20581 ggaggaggcg gtggctgctg cggccgccgc gaggaccgca ggccagtcct ctccaggcag
    20641 tgccgaccag acggcacggg gcgcttgtcc gtcagcaagg cgacgaagca gcgcctcacc
    20701 acctcgatgt cgagaccagc ccgcctgacc gggcgtcccc gtcagcaacg catcgacggg
    20761 aaggtcgagc cgggtctcga tgggcgtcgt cggctcgctc tcgacacgag cgtggcgggg
    20821 cggcaccgcc agacgtagca cgtcagcgag cgtcccggcg taccggtccg ccaccatccg
    20881 ggccgtggtg agcacctccg catggaggac aggctcgcgc gacacgaccc tgcgcagcgc
    20941 ggcgagctcc ccgacatggg tggtctcttc ggctcgctcg acgacaaagc cgtccacgta
    21001 gcggccggcg aaccgcacct tcacccggca tccggcctgc gcttcctcgt gcagggcgag
    21061 tggcaccaga tagtcgaatg tctggtcgag gtgcggcagc ggcaggtcga ccgcgacacg
    21121 cgcaaccggc agcgtcgggg ccggtccggt gacgggtagc cgacgccggc cccgcacctg
    21181 tgtccgcagc agtgccaact gctcaccagg cccactcatc gcagacctgt tctaccagcc
    21241 gggtacgaca ccggaccgaa ctaccggctg ggactaccgg ctcggctgtc cctggagcca
    21301 ggccttgccg cggctcggcg acgcacgggc gagccaggca tcctggatca cctccgccag
    21361 ctcctcgacc gtcagctcgc ccaagcggct ggcccgaacc agcaccgacc tgtgaccgtt
    21421 gaagtgcggc gtcgtgaaga acggactgct ctcgtcctgg acgagcgcct ccttgtccgc
    21481 ctctgagccg acccagatga cgatcacgtc ggcatagcgt tgtttggtct ccgggtcgaa
    21541 cgcgtccgga cgcgggttgc ggaagaagac gaacgacttg ccgccgacct ggtacaccgg
    21601 gttgtcgtcc gtgcccgggt agaccgtcac gtgcggcatc ccggccgcga gctcgtgcac
    21661 atcgtcgaca cgggctggtc ggtttcggct cggcacacga gcagactacg gcggcctccg
    21721 attcgtccga ccagctcggt ggtggtttgc ctgggtagcc atccaaccgg acgggtagcc
    21781 aggcagatct gcgttgggag
//
LOCUS       DATBFZ010000481         2038 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_5749753, whole genome shotgun sequence.
ACCESSION   DATBFZ010000481 DATBFZ010000000
VERSION     DATBFZ010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5749753"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            166..2004
                     /locus_tag="VH419_14670"
     CDS             166..2004
                     /locus_tag="VH419_14670"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S53 family peptidase"
                     /protein_id="HEY7044909.1"
                     /translation="MNKANALGATPSAQQVNFGVLLSMRDQAGAQAALQAISDPTSAS
                     YGQWLSNAAFDAKYAPAAADVAAVQGWLRSQGFQVTKTLPSGMYVEASGSVAQVEQTF
                     GTEVNNYSYLGKTVRANATNLSLPASTPAAVSGVIAGVVGIDQGSLLKQPADTEPGPP
                     DGARYGVQPCSNYYGQKVAYDKPPAYGRTEPYVICGYVPQQYQSAYGESGLLAAGVNG
                     RGVTVAITDAYAAPTIYQDAQRYNQVHHQPLFARGQFSQITPGPNGYDNVDLCGAQGW
                     YGEETLDVEAVHAMAPGAKVVYVGASDCLSGLDDAWANAIDNHVADVITNSWTDGTDD
                     INLLGSDYVAFYQQFSLEAALTGITVNFSSGDDGDHTAGGTDLSSKTVEFPADLPYVT
                     GVGGTSVEIGGRGQWLNEYGWQSAYSTLDNGAWDPTPPGPYSSGGGGGTSQLFAQPFY
                     QVGRVPASISRYYGGAPMRAVPDISMPGDPNTGFEVGETQVFPDGTYWDQYRIGGTSL
                     SSPLMAGVVAVADQLNRRPLGFINPLYYRLLGTSALHDIVAPRSPVAEVRTDYANFLD
                     SSDGFLFRLRTTDVQSSTLHDTPGYDDETGVGTPNGPAFFIGAARH"
BASE COUNT          344 a          656 c          696 g          342 t
ORIGIN      
        1 acgacccgaa aaacgtgggg agtagatatg agatcgtctc gatttgtcag acgggggttg
       61 cttgtaggcg tgcttgggtg cgtcgcgctg gtcgcggcca ccatcgttgc cacagcctcg
      121 gcgtctgccg gtcggaaaca gctgcgcggc agtagtcccg gttggttgaa caaggccaat
      181 gctcttggtg ccacgccgtc ggcgcagcag gtgaacttcg gcgtgctgct gagcatgcgt
      241 gaccaggccg gagcgcaggc cgcgctgcag gccatctccg atccgacgag cgccagctat
      301 ggccagtggt tgtcgaacgc agcgttcgat gccaagtatg cgcctgctgc cgccgacgtg
      361 gcggccgtgc agggctggct tcgctcgcag ggcttccagg tcaccaagac gttgccgagc
      421 gggatgtacg tggaggccag cggctcggtc gcgcaggtgg agcagacgtt cgggaccgag
      481 gtgaacaact actcctacct gggcaagacg gtgcgtgcca acgccacgaa cttgtcgctg
      541 ccggccagca ccccggcggc ggtcagtggc gtgatcgcgg gtgtggtcgg gatcgatcag
      601 ggttcgttgt tgaagcagcc tgccgacacg gagcccggac ctccggacgg ggctcggtac
      661 ggggtgcagc cgtgctcgaa ctactacggc cagaaggtcg cgtacgacaa gccgcccgct
      721 tacggccgga ccgagccgta cgtgatctgc gggtacgtgc cccagcagta ccagtcggcc
      781 tatggcgaga gcgggcttct ggctgccggc gtgaacggcc gcggggtcac cgtggcgatc
      841 accgacgcat acgccgctcc gacgatctat caggacgccc agcggtacaa ccaggtgcac
      901 catcagccgc tgttcgctcg tggccagttc tcccagatca ctcctggtcc caacggctat
      961 gacaacgtcg atttgtgcgg cgcccagggc tggtacggag aggaaaccct cgatgtggaa
     1021 gccgtgcacg cgatggcgcc aggcgccaag gttgtctatg tcggcgcgtc ggactgtctg
     1081 agtgggctcg acgacgcctg ggcgaacgcg atcgacaacc acgtcgccga cgtgatcacc
     1141 aactcatgga ccgacggcac ggacgacatc aacctgctcg ggtccgatta cgtcgcgttc
     1201 tatcagcagt tctcgctcga ggcggcgctg accggtatta cggtcaactt ctcctccggc
     1261 gatgacggcg accacactgc cggcggtacg gacctgtcct cgaagaccgt ggagttcccc
     1321 gcggacctgc cgtatgtcac cggcgttggc ggcacgagcg tcgagatcgg cggcaggggc
     1381 cagtggctga acgagtacgg atggcagagc gcgtactcga cgctcgacaa cggtgcgtgg
     1441 gatccgacgc cccccgggcc ttactcctcc ggcggcggcg gcgggacgag ccagctgttc
     1501 gcgcagccgt tctaccaggt cggcagagtt ccggcgagca tctcccggta ctatggtggc
     1561 gccccgatgc gggcggtacc agacatctcg atgccgggcg atcccaacac cggcttcgag
     1621 gtcggcgaga cccaggtgtt ccccgacggc acgtactggg accagtaccg catcggcgga
     1681 accagcctgt cgtccccgct gatggccggc gtggtagcgg tcgccgacca gctcaaccgt
     1741 cgaccgctcg ggttcatcaa tccgctgtat taccggctgc tcggcacgag cgcgctgcac
     1801 gacatcgtcg cgccgcggtc gccggtggcg gaggtgcgta cggactacgc gaacttcctg
     1861 gacagcagcg atggtttcct gttccggttg cggacaaccg acgtccagag ctcgacgctc
     1921 cacgacacgc cgggatacga cgacgagacg ggcgtgggta cgcctaacgg ccccgcgttc
     1981 ttcatcgggg ccgcgaggca ctgacataag tcggcgccgt cccaccgtcc gcatgagg
//
LOCUS       DATBFZ010000482         4011 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_5434519, whole genome shotgun sequence.
ACCESSION   DATBFZ010000482 DATBFZ010000000
VERSION     DATBFZ010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 4011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4011
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5434519"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            88..1245
                     /locus_tag="VH419_14675"
     CDS             88..1245
                     /locus_tag="VH419_14675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009083725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY7044910.1"
                     /translation="MSSFELSAQQQEHLAWVKDIARTTLLPMAERGEPGRVNRELIAA
                     MGELGLLPRLFPGVGEDETTSREAAALDLCLLREALATVSTEAETALALQGLGTYPVL
                     QSGSDELVRRWVPAVARGDAVAGFALTEPEAGSDAAALALDAKPDGDGWRLTGEKVWI
                     SNAPEADVYSVFARTTAEAGARGVTAFAVRGDADGLTGEHLDMLSPHPIGRLVFDGVR
                     VDRADLLGEPDRGFAVAMRTLDLFRPSVGAFAVGMAQAALDAALAYADERQAFGGPLR
                     EMQSVAHTLAEMATRVESARLMVYRAGAAYDAGRPAISTTSAMAKLVATETAQWVVDR
                     AIQIHGARALERGHLLEHLYREVRAPRIYEGATEVQRSIIARDLYRLVRNP"
     gene            complement(1259..2947)
                     /locus_tag="VH419_14680"
     CDS             complement(1259..2947)
                     /locus_tag="VH419_14680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017936275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY7044911.1"
                     /translation="MSRDLDCRHGEGILSGIMAADPITLSPSAHVDTFCRDSLPPPES
                     WPEFVFDLEDVQYPDRLNCAVELLDRTIAAHGADRTCLLAPNGERWTYGELRSTANRI
                     ARVLVDDLGIVPGNRVLLRGPNNPWLVACWFGVLRAGAVVVTTMPLLRPGELSTVHEI
                     ARIDVSLCDHRFLDDLRASDLGTVPLVPYGGDADDDLGRRVLQVSHEFEPVDTAADDV
                     ALLAFTSGTTGRPKATMHFHRDVLANADTFSKHVLRPTSDDVFTGTPPLAFTFGLGGL
                     VVFPLRVGASSLLIEKATPAELAEHIGAHDVTICFTAPTAYRAMLTAADISAVSSLRR
                     CVSAGEHLPVATWTAFHEATGVKIIDGIGSTELLHIFISAADDAIVPGSTGVPVPGFQ
                     AEVVDADGKPLPAGESGRLAVKGPTGCRYLADPRQAAYVQNGWNITGDTFVKDDNGYF
                     WYQARSDDMIISSGYNIAGPEVEEAVLRHPDVVECAVVGTPDEDRGQLVTAYVVLRDG
                     AVGDDTFVKALQDFTKGEIAPYKYPRAVHVVDSLPRTSTGKLQRFRLRDRVVRS"
     gene            2966..3808
                     /locus_tag="VH419_14685"
     CDS             2966..3808
                     /locus_tag="VH419_14685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009083718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaX family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HEY7044912.1"
                     /translation="MSIARTDPNNAFQPRALIVTVYGLYARDATGWLSVSALIRLMAE
                     LGVDEAAVRSSISRLKRRGILQARARDGAAGYALSGTARSLLDSGDRRIFERPRAAPG
                     EAWLLAVFSVPESERSRRHALRSRLTWLGFGTVAAGVWIAPAHLEAETRDALERDDLT
                     AYVDLFRADYLDFGKVGEQVRTWWDLTGLQGLYDDFLSAYGPVLTRWRRRRATSGTSA
                     DADAFADYVSTLTAWRRLPYLDPGLPDDVLPKGWSGARAADLFFDLRDRLAEPAAAFV
                     TTVR"
BASE COUNT          607 a         1413 c         1386 g          605 t
ORIGIN      
        1 tgatgaaggg tgacgactac gccgagttcc atgccgcgtt caccggcagg cgaccgcccg
       61 agtggaccgg gcgctgacat ggagggcatg agcagcttcg agctctccgc acagcagcag
      121 gagcacctcg cctgggtgaa agacatcgcc cggaccaccc tgctgcccat ggccgaacgc
      181 ggcgagccgg gccgcgtcaa ccgcgagctc atcgcggcga tgggtgagct cggcctgctg
      241 ccgcgcctgt tccctggtgt gggcgaggac gagacgacgt cccgggaggc cgcggccctg
      301 gacctgtgtc tcctgcggga ggcgctcgcg accgtcagca ccgaggccga gacggcgctc
      361 gccctgcagg gactggggac ataccccgtg ctgcagtccg gatcggacga gctggtccgc
      421 cggtgggtgc cggcggtcgc ccgcggggac gccgtcgcag gattcgcgct gaccgaaccg
      481 gaggccggtt cggatgccgc ggcgcttgca ctggacgcca agcccgacgg cgacgggtgg
      541 cggctgaccg gtgagaaggt ctggatctcg aacgctcccg aggccgacgt ctactcggtg
      601 ttcgcacgca ccaccgccga agcgggtgct cgtggcgtga ccgccttcgc cgttcgagga
      661 gacgccgacg gactgaccgg tgagcacctc gacatgctgt cgccgcaccc gatcggccgg
      721 ctcgtcttcg acggcgtacg agtcgaccgt gcggacctgc tgggcgaacc cgaccgcggc
      781 ttcgccgtgg ccatgcgcac gctcgacctg ttccggccga gcgtgggcgc gttcgcggtc
      841 ggcatggcgc aggctgcgct cgacgcggca ctcgcgtatg ccgacgagcg ccaggcgttc
      901 ggcggaccgc tgcgcgagat gcagtcggtg gcccacacgc tggcggagat ggcgacccgg
      961 gtcgagtcag cccggctgat ggtctaccgc gccggagcgg cgtacgacgc gggccgcccg
     1021 gccatctcga cgacctcggc catggcgaaa ctggtcgcca ccgagaccgc acagtgggtc
     1081 gtcgaccggg ccatccagat ccatggcgcc cgcgcactcg aacgcggcca cctgctcgag
     1141 cacctgtacc gggaggtccg ggctccgcgc atctacgagg gcgccaccga ggtccagcgc
     1201 tcgatcatcg cccgcgacct ttaccgactt gtcagaaacc catagaccta tagccccatc
     1261 agcttctgac aactcggtcg cggaggcgga agcgctgcag cttgccggtg ctcgtgcggg
     1321 gcagcgagtc gaccacgtgg acggcacgcg ggtacttgta cggcgcgatc tcgcccttcg
     1381 tgaagtcctg cagcgccttg acgaaggtgt cgtcaccgac cgcgccgtcg cgcaggacga
     1441 cgtacgccgt cacgagctgc ccgcgatcct cgtccggagt cccgacgacc gcacactcga
     1501 ccacgtccgg atgccgcagc accgcttcct ccacctctgg tccagcgatg ttgtatccgg
     1561 aggagatgat catgtcgtcg ctgcgcgcct ggtaccagaa atagccgttg tcgtccttga
     1621 cgaacgtgtc gccggtgatg ttccagccgt tctgcacgta cgccgcctgg cgaggatccg
     1681 cgaggtagcg gcacccggtc ggacccttga cggcaagccg cccggactcg cccgccggga
     1741 gcggcttccc gtccgcgtcg acgacctcgg cctggaagcc ggggaccggg acacccgtcg
     1801 agccgggcac gatggcgtcg tctgctgccg agatgaagat gtgcagcagc tcggtcgaac
     1861 cgatgccgtc gatgatcttc acgccggtcg cctcgtggaa cgccgtccac gtcgccaccg
     1921 gcaggtgctc gcccgcggac acacagcgtc gtaggctcga cacggcagat atgtcggccg
     1981 cggtgagcat cgcccggtac gccgtgggag cggtgaagca gatcgtcacg tcgtgcgcgc
     2041 cgatgtgctc ggccagctcg gccggcgtgg ccttctcgat cagcagcgac gatgcaccga
     2101 cccgcagcgg gaacacgacc agcccgccga gcccgaacgt gaacgccagc ggcggcgtcc
     2161 cggtgaagac gtcgtcgctc gtcggtcgca gcacgtgctt ggagaacgtg tcggcgttgg
     2221 ccagcacgtc acgatggaag tgcatcgtcg ccttggggcg acccgtcgtt cctgaggtga
     2281 acgccaacaa cgccacgtcg tcggcagccg tgtcgacggg ctcgaactcg tgtgacacct
     2341 gcaggacgcg gcggccgaga tcgtcgtcgg cgtcgccgcc gtacggcacc agcgggaccg
     2401 tgccgaggtc cgacgcccgc aggtcgtcga ggaacctgtg gtcgcacagc gacacgtcga
     2461 tccgggcgat ctcgtgcacc gtgctgagct caccgggacg taacagcggc atcgtcgtca
     2521 cgacgaccgc ccccgcgcgc agcacgccga accagcaggc gaccagccac gggttgttcg
     2581 gtccgcgcaa cagcacccgg ttgcccggca cgatgccgag gtcgtcgacg aggacccggg
     2641 cgatccggtt ggccgtcgac cgcagctcgc cgtacgtcca ccgctcaccg ttcggcgcca
     2701 gcaggcaggt ccggtcggcg ccatgggcgg cgatggtcct gtcgagcaac tcgacggcgc
     2761 agttcagccg gtcggggtac tgcacgtcct cgaggtcgaa gacgaactcc ggccatgatt
     2821 ccgggggcgg caggctgtcg cgacagaagg tgtcgacatg cgcggacggg ctcagcgtga
     2881 tcgggtctgc ggccatgatg ccgcttagta ttccctcccc atgacgacag tcaaggtcac
     2941 gcgacacgcg cgctatcgtg ctggcgtgtc catcgcgcgc acggacccga acaatgcctt
     3001 ccagccgcgc gcgctgatcg tcacggtcta cgggctgtac gcgcgggatg cgaccgggtg
     3061 gctcagcgtg tctgcattga tccgcctgat ggccgagctc ggcgtcgacg aggcggctgt
     3121 ccggtcatcg atctcgcggc tgaagcggcg cggcatcctc caggcgcgag cacgtgacgg
     3181 cgcggccggt tacgcgctct cggggaccgc gcgatcgctg ctggactccg gagaccgccg
     3241 catcttcgag cgaccgcgcg ccgcccctgg tgaggcgtgg ctgctggcgg tgttcagcgt
     3301 gcccgagtcc gaacgcagcc gccggcatgc gctgcgttcc aggctgacct ggctgggttt
     3361 cggcacggtc gcggccggtg tctggatcgc gcccgcgcac ctcgaggccg agacacgaga
     3421 cgcgctcgaa cgcgatgacc tgacggcgta cgtcgatctc ttccgtgccg actacctgga
     3481 cttcggcaag gtcggcgagc aggtccggac ctggtgggac ctcacgggtc tgcaggggct
     3541 gtacgacgac ttcctgtcgg cgtacggccc cgtcctcacg cgctggaggc gacgccgcgc
     3601 aacctcgggg acttcggccg acgccgacgc gttcgccgat tacgtcagca cgctcacagc
     3661 ctggcgccgg ctgccctacc tggacccggg actccccgac gacgtcctcc ccaaaggctg
     3721 gtccggcgcc cgtgcggccg acctcttctt cgacctgcgc gaccgcctcg ccgaaccggc
     3781 ggccgccttc gtcacaaccg ttcgctgagg cgatgggccg ggaagctacc cgagtcttcc
     3841 gggtctcgac aagctcgacc gccggatatt cccggcccgc tgatggagcc cgtcgagatc
     3901 accgggtgac ggctcgacaa gctcgaccgc cgcaggccgt tcagacgtcg gatcgtgcct
     3961 cgacgcgacc atcgacgacc gcagccgaca gcgtggcgtg gtcgcgctcg t
//
LOCUS       DATBFZ010000483         3483 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_4777338, whole genome shotgun sequence.
ACCESSION   DATBFZ010000483 DATBFZ010000000
VERSION     DATBFZ010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 3483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3483
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4777338"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..919
                     /locus_tag="VH419_14690"
     CDS             <1..919
                     /locus_tag="VH419_14690"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEY7044913.1"
                     /translation="LAIFVLLSIAVLGRGWAAWWQMLRDWLPFYAVLLVYDYSRGFAS
                     PYSPEQMANRQYPIQDVHNGLGLPLHVQFPIRADLWIGQHLGMGGMPTTWVQEHLHPG
                     VQEPWYAVLASLTYCSHFLVMPTVAVVLWLTNRGRFRVWMRMVVALAVAGVATYFLYP
                     MAPPWLADHQGVFPGPPVGRYTSEGFDLIGLHMVGGALDQGQYLVNPVAAMPSLHMAY
                     ATLVVGFFWFGKRWWQRCLLALYPLAMGITLVYGGEHYVVDEIAGVAYALVIIAVSRY
                     IHVRRLRQASSVELVETTTGRHRPHPTLG"
     gene            complement(901..2193)
                     /locus_tag="VH419_14695"
     CDS             complement(901..2193)
                     /locus_tag="VH419_14695"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008363769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY7044914.1"
                     /translation="MVKIADAFAQLLEGPLPLRFTAYDGSASGPEGAPFGMELKNERG
                     LAYLMTAPGDLGLGRAYVSGDLVVTGIHPGDPYPALAVLQKGLKFRTPSPTEVVKLAS
                     GLGLSHLKPPAPPPQEHLPRWRRVAEGLRHSRKRDSEAIHHHYDVSNTFYEYVLGPSM
                     TYTCAVFPTEEATLEQAQYYKYDLVARKLGLEPGMRLLDVGCGWGGMVRHAAEHYGVT
                     ALGVTLSREQATWAQEKIKEQGLDHLAEVRHGDYREVTEGDFDAVSSIGLTEHIGVRN
                     YKSYFSFLRDRLRPRGRLLNHCITRPHNRHQSTGAFIDRYVFPDGELTGSGRIITEVQ
                     NTGLEVMHEENFRLHYARTLAGWCHNLAENWDACVAEVGEGTARVWGLYMAGSRLAFE
                     RNEIQLHHVLAVKTDEEGHDDFPVRPTFPMEASYPSVG"
     gene            complement(2225..>3483)
                     /locus_tag="VH419_14700"
     CDS             complement(2225..>3483)
                     /locus_tag="VH419_14700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017935633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY7044915.1"
                     /translation="TDSPGLDVSGLSGVISIDEEAQTADVQGMCTYEDLVDATLPRGF
                     IPYVVPQLRTITLGGAVSGLGIESTSFRNGLPHESVVEMDIFTGAGEVVTARPAPDDE
                     HHDLFDTFPNSYGSLGYATRLRIKLERVPSYVDLRHVRFDDAGVLAKTISQIMETREY
                     EGVRVDGLDGTAFQPGEYYLSLATWTDRVAIAPSDYTGQDIYYRSIQRLAAAGSGQDA
                     LTMHDYLWRWDTDWFWCSRAFGAQHPVVRRFWPRRYKRSDVYYRMVQFDRRLHVGAWL
                     DRRRGNPEGERVVQDVEIPVERLGEFLDWFDHEVGMRPVWLCPLTAAKKWPSYPLEPG
                     EVYVNVGFWGNVGVGPEAPNAPRNRAVEAKVHELDGHKSLYSEAFYDRETFDRLYDGD
                     NLARVKDRYDPGQRLTSLYDKAVRRR"
BASE COUNT          504 a         1205 c         1188 g          586 t
ORIGIN      
        1 cctcgcgatc ttcgtcctgc tgtcgatcgc ggtgctcggc cggggctggg cggcgtggtg
       61 gcagatgctg cgcgactggc tccccttcta cgccgtcctg ctggtctacg actacagccg
      121 cggcttcgcc tcgccgtact caccggagca gatggcgaac cgtcagtacc cgatccagga
      181 cgtgcacaac ggtctcggac tgcctctgca cgtccagttc ccgatccgcg ccgacctgtg
      241 gatcgggcag cacctcggca tgggcggcat gccgacgacg tgggtccagg agcacctgca
      301 ccccggcgtc caggaaccct ggtacgccgt cctggcgagc ctcacctact gcagccactt
      361 cctggtgatg ccgaccgtgg ccgtcgtcct ctggttgacc aaccgcggac gtttccgggt
      421 ctggatgcgg atggtcgtgg cactggccgt cgcgggcgtc gcgacgtact tcctgtaccc
      481 gatggcgccg ccctggctgg cggaccacca aggggtcttc cccgggccac ccgtcggtcg
      541 gtacacgtcc gagggcttcg acctgatcgg cctgcacatg gtgggcggcg cgctggacca
      601 ggggcagtac ctggtgaacc cggtggcggc gatgccgtcg ctgcacatgg cgtacgcgac
      661 gctggtcgtc gggttcttct ggttcgggaa gcggtggtgg cagcgctgtc tgctcgctct
      721 ctaccccctc gcgatgggga tcacgctggt gtacggcggc gagcactacg tcgtcgacga
      781 gatcgccggc gtcgcctacg cgctcgtgat catcgccgtg tctcgctaca ttcacgtgcg
      841 ccggctcaga caggcgtcgt cggtcgagct tgtcgagacc acgaccggca ggcaccgacc
      901 tcatccaacg ctcgggtagc tcgcctccat cgggaacgtc ggccggaccg ggaagtcgtc
      961 gtggccttcc tcgtcggtct tcaccgcgag cacgtggtgg agctggatct cgttgcgctc
     1021 gaacgcgagc cgtgacccgg ccatgtacag cccccagacc ctggctgtcc cctcgccgac
     1081 ctcggcgacg caggcgtccc agttctcggc gaggttgtgg caccagcccg ccagcgtcct
     1141 ggcgtagtgg aggcggaagt tctcctcgtg catcacctcg agaccggtgt tctgcacctc
     1201 ggtgatgatc cggccggacc cggtcagctc cccgtcgggg aacacgtacc ggtcgatgaa
     1261 cgcacccgtc gactggtgcc ggttgtgcgg ccgggtgatg cagtggttga gtaaccggcc
     1321 ccgaggacgc agacggtcgc gcaggaacga gaagtacgac ttgtagttgc ggacgccgat
     1381 gtgctcggtg aggccgatcg aggagaccgc gtcgaagtcg ccctcggtca cctcgcggta
     1441 gtcgccgtgg cgcacctcgg cgaggtggtc gagcccctgc tccttgatct tctcctgcgc
     1501 ccacgtcgcc tgctcgcgcg acagggtcac gcccagggcc gtcacgccgt agtgctcggc
     1561 ggcgtggcgg accatgccac cccaaccgca cccgacgtcg agcaggcgca tgcccggctc
     1621 gagcccgagc ttcctggcca ccaggtcgta cttgtagtac tgcgcctgct ccagcgtggc
     1681 ctcctcggtc gggaagaccg cgcacgtgta ggtcatcgac ggacccagga cgtactcgta
     1741 gaacgtgttc gacacgtcgt agtggtggtg gatggcctcg gagtcacgtt tgcgtgagtg
     1801 acgcaagccc tccgcgacgc gacgccagcg cggcaggtgc tcctgcggcg gcggggccgg
     1861 aggcttgagg tgagagagcc ccaggccgga ggcgagcttg accacctccg tcggcgacgg
     1921 ggtccggaac ttcagcccct tctgcaggac cgcgagcgcg gggtatgggt cgccggggtg
     1981 gatgccggtc accacgaggt cgccggacac gtaggcacgg cccagtccga ggtcgccggg
     2041 cgcggtcatc aggtacgcga ggccgcgctc gttcttcagc tccatgccga acggcgcacc
     2101 ctccggcccg ctcgcgctcc cgtcgtacgc ggtgaaccgg agcggcaacg ggccctcgag
     2161 cagctgcgcg aacgcgtctg cgatcttcac catgccatca cctcgcacat ctcctcgcca
     2221 ggcatcatcg tctcctcacg gccttgtcgt acaggctggt cagccgctga cccgggtcgt
     2281 accgatcctt cacgcgcgcc aggttgtcgc cgtcgtagag ccggtcgaag gtctcccggt
     2341 cgtagaaggc ctccgagtac agcgacttgt ggccgtcgag ctcgtgcacc ttcgcctcga
     2401 cggcccggtt gcgcggcgcg ttcggtgcct cggggccgac acccacgttg ccccagaacc
     2461 cgacgttgac gtacacctcg ccgggctcga gcgggtacga cggccacttc ttcgccgcgg
     2521 tcagcgggca cagccagacc ggacgcatcc cgacctcgtg gtcgaaccag tcgaggaact
     2581 cccccagccg ctcgaccggg atctcgacgt cctgcaccac ccgctcgccc tcggggttgc
     2641 cgcggcgacg gtcgagccag gcaccgacgt ggagacgccg gtcgaactgg accatccggt
     2701 agtagacgtc gctgcgcttg tagcgccggg gccagaaccg tcgcacgacg gggtgctgcg
     2761 cgccgaaggc acgggagcac cagaaccagt cggtgtccca acgccacagg tagtcgtgca
     2821 tcgtgagcgc gtcctgcccg gatcctgcag cggcgagacg ctggatcgag cggtagtaga
     2881 tgtcctggcc cgtgtagtcg ctgggcgcga tcgccacccg gtcggtccac gtcgccaggg
     2941 acaggtagta ctcccccggc tggaacgccg tgccgtccag accgtcgaca cggacgcctt
     3001 cgtactcccg ggtctccatg atctgggaga tcgtcttggc cagcaccccg gcgtcgtcga
     3061 agcgcacgtg ccggaggtcg acgtacgacg gcacccgttc gagcttgatc cgcagccgcg
     3121 tggcgtagcc gagcgagccg tagctgttgg ggaacgtgtc gaagagatcg tggtgctcgt
     3181 cgtccggggc gggccgggcc gtcacgacct caccggcgcc ggtgaagatg tccatctcga
     3241 cgacggactc gtgcggcaac ccgttgcgga agctcgtcga ctcgatgccg aggccgctca
     3301 cggcgccgcc gagggtgatc gtgcgcagct gcgggacgac gtaggggatg aagccgcggg
     3361 gcagcgtcgc gtcgacgagg tcctcatagg tgcacatgcc ctggacgtcg gcggtctgcg
     3421 cctcctcgtc gatcgagatg acgccgctca gcccggagac gtccaggccc ggcgagtcgg
     3481 tcg
//
LOCUS       DATBFZ010000484         3564 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_6411069, whole genome shotgun sequence.
ACCESSION   DATBFZ010000484 DATBFZ010000000
VERSION     DATBFZ010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 3564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3564
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6411069"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..643
                     /locus_tag="VH419_14705"
     CDS             <1..643
                     /locus_tag="VH419_14705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044916.1"
                     /translation="MVALHDAASSTMGAKPGQTTLSFEANDIDALATRLRAAEVPGVA
                     VVDEAYGRVLSCKDPLGDEIVIDERTGDLYGYRTHPESRPEAGLAVVPVRFTAPQGPY
                     GVWLEALGMTRVGDLDEFYVMWDAGDAKHGYVGLHYVYVDDESELPIVPGPASVHLTF
                     ATAEPLDEVAGRLVQAGHADARVSVEDFGSMLSVTDPDGQEVQVHERPAADAA"
     gene            640..1953
                     /locus_tag="VH419_14710"
     CDS             640..1953
                     /locus_tag="VH419_14710"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY7044917.1"
                     /translation="MTDPGNPTTRLRDDADFRRYWWARLLSLSGTVVTLVALPVLVYR
                     LSGSAFLTALVSALEAVPYLLFGLFAGALADRLDRRAVMVAADLANALVMGSVPLAHW
                     LGVLTVPHVLAVAFLTPAIAVFFDGANFGALPVLVGRDRIATANAVVWSAATSVEIVL
                     PALVGVALAVVDPASLLLLDALSFVASGLCVRAITRLLHDPWRPRGPLTRRVLFGDIG
                     AGLHFLVHHAGVRTMTMVGTVQCLAGGGFVALLVVWCDRILGVGTEGVRFGLVYAGWS
                     VGALAAAALLPRILRRSSAAVVALAALPFSAVLGILTSFATWWPLAALGLMCWGSVYT
                     LVVVNSISYRQEVTPEALLGRVNTAGRMLSWGLGWTVGALFAGWLSHQIGVRPALTTM
                     ASLGLVAVAVAWTSPLRREQGEPATNSARHAADSVGIAGEQGTAR"
     gene            1964..3214
                     /locus_tag="VH419_14715"
     CDS             1964..3214
                     /locus_tag="VH419_14715"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEY7044918.1"
                     /translation="MNPLGRSSARAALSVVAAAAISLAALEAPPSAAGLRASVDSGVP
                     SATGASALAGVAGHHQRKRLPALRTHVVMPHLDIPWDISVMPSGAWLVTERDRQRILL
                     HLPGGGRRVLSNGPAGIWSSGETGLMSIVHDPRVARNNRFYTCNGWERNGHHDIRVLA
                     WRLNRGRTHARIVDTLVSGIQITSGRHGGCRLRFDRAGALWVGTGDSTVGTNPQNLHS
                     LNGKLLRLNRFSGRPWPTNPWVHAPNRHKRFIFNYGHRNVQGLAVRPGGGIWSVEHGT
                     DRDDEVNRGARGGNFGWNPVPGYDESTPMTDFSLPGRQIGARWRSGFPTLATSGAVWV
                     RGKKWGRYNGTLAVCALKASELLFMKFDRHGRFRWMRTPPAMRGRYGRLRSVALTHNH
                     DLLVTTSNGGGGSGGDRVIRVSPR"
     gene            complement(3313..>3564)
                     /locus_tag="VH419_14720"
     CDS             complement(3313..>3564)
                     /locus_tag="VH419_14720"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY7044919.1"
                     /translation="RVVFKAELGAVTPHAAHLQSVWIDPSFRGQGLAESRVAAVCQLA
                     LGDVAPVVSLYVNQHNEPARRTYERVGFVTTGTFATILF"
BASE COUNT          489 a         1222 c         1254 g          599 t
ORIGIN      
        1 gatggtcgcg ctccatgacg cggcgtcctc gaccatgggc gcgaagccgg gacagaccac
       61 cttgagcttc gaggcgaacg acatcgacgc gctggcgacg cggcttcgcg ccgccgaggt
      121 tccgggcgtg gcggtcgtcg acgaggcgta cggacgtgtg ctcagctgca aggacccgct
      181 cggtgacgag atcgtgatcg acgagcgcac cggcgacctg tacggctacc gcacgcaccc
      241 cgagtcgcgc cccgaagccg gccttgccgt cgtgcctgtt cgcttcaccg cgccgcaggg
      301 tccctacggc gtttggctcg aggccctcgg catgacccgg gtgggcgatc tggacgagtt
      361 ctacgtgatg tgggacgccg gagacgcgaa gcacgggtac gtcggcttgc actacgtcta
      421 tgtcgacgac gagtccgaac tgccgatcgt gcccggcccc gcgtcggtgc acctgacgtt
      481 cgccacggcc gaaccgctgg acgaagtggc cgggcgcctg gtccaggccg ggcacgccga
      541 tgcgcgcgtg tcggtcgagg acttcgggtc gatgctgtcc gtcacggatc ccgacggcca
      601 ggaggtccag gtccacgaac ggccggccgc ggatgccgca tgacggaccc tgggaaccct
      661 acgacgcgcc ttcgcgacga cgctgacttc cgccgctact ggtgggcgcg cctgttgtca
      721 ctgagtggca cggtcgtgac gctcgtggcg ctgcccgtcc tggtgtaccg gttgagcgga
      781 tcggccttcc tcacggcgct agtgtcggcg ctggaggccg tgccgtacct gctgttcggt
      841 ctgttcgctg gtgcgctggc cgaccggctg gaccgtcggg cggtgatggt tgctgcggac
      901 ctcgccaacg ctctggtgat gggttccgtg ccgctcgctc actggctcgg cgtcctcacc
      961 gtcccgcacg tcctggccgt cgcgttcctg accccggcca tcgcggtgtt cttcgacggt
     1021 gccaacttcg gggcgctgcc ggtgctggtc ggccgggacc ggatcgcgac cgcgaacgcc
     1081 gtcgtgtgga gcgcggccac ctcggtcgag atcgtgcttc ccgcgctggt cggcgtggcg
     1141 ctcgccgtcg tcgacccggc gtcgctgctg ctgctggatg cgttgagctt cgttgcgtcc
     1201 gggctctgcg tccgggcgat caccaggctg ctgcacgacc cgtggaggcc acgcggcccg
     1261 ctcacccggc gcgtgctgtt cggcgacatc ggcgccgggc tccactttct cgtccaccat
     1321 gccggcgtac gcacgatgac gatggtcggc accgtgcagt gcctggccgg cggcggcttc
     1381 gtggccctgc tggtcgtgtg gtgcgaccgc atcctcggtg tcggcaccga gggtgtccgg
     1441 ttcggtctgg tgtacgccgg atggagtgtc ggcgcgctgg ccgccgctgc attgcttcca
     1501 cggatcttgc gcaggtcgag tgcagccgtg gtcgcgttgg cggccctccc gttctcggcg
     1561 gtcttgggca tcctgaccag cttcgcgacc tggtggccgc tggcggcgct cggactgatg
     1621 tgctggggca gcgtctacac actggtcgtc gtcaactcga tctcctaccg acaggaggtg
     1681 acgccggagg cgttgctcgg ccgggtgaac actgccggcc ggatgctgtc gtgggggttg
     1741 ggctggaccg tcggggcact cttcgccggc tggctgagcc accagatcgg cgtacgaccg
     1801 gccctgacga cgatggcgtc gctgggcctg gtcgcggtcg ccgtggcctg gacgtctccg
     1861 ctgcggcgcg aacaaggaga gccggcgacg aacagcgcgc gacacgctgc cgactccgtt
     1921 gggatcgctg gcgagcaggg caccgcacgt tgatactgag ccgatgaatc ccctgggaag
     1981 gtcctccgct cgcgcagcac tcagcgtcgt cgccgcggcg gcgatcagtc tcgcggcgct
     2041 ggaagctccg ccgtcggccg ccggtctccg cgcgtccgtg gacagtggcg tcccctcggc
     2101 gaccggtgcg tccgcgctcg ctggggtcgc cggccaccac cagcgcaagc gactgccggc
     2161 gctgcgcacg catgtcgtga tgccacatct ggacattccc tgggacatct ccgtcatgcc
     2221 gagcggagca tggctggtca ccgagcgcga ccgccagcgc atcctgctgc accttccagg
     2281 cggcggtcgg cgagtcctca gtaacggtcc ggccggcatc tggtccagtg gcgagaccgg
     2341 cctcatgtcg atcgtccacg acccacgggt ggcgaggaac aaccgcttct acacctgcaa
     2401 cggttgggag cgcaacggcc accacgacat ccgcgtgctc gcctggcggc tcaaccgggg
     2461 gcggacccac gctcgcatcg tcgacacgtt ggtgagcggc atccagatca cgtcgggtcg
     2521 acacggtggt tgtcggctcc gcttcgaccg agccggtgcg ttgtgggtgg gcaccggcga
     2581 ctccacggtc ggaaccaacc cgcagaacct gcacagcctc aacggaaagc tgttgcggct
     2641 gaaccggttc agcgggcgtc cgtggccgac gaacccgtgg gtccacgcgc cgaaccggca
     2701 caagcgcttc atcttcaact acggccaccg caacgtgcag ggacttgcgg tgcggccagg
     2761 cggtggcatc tggtccgtcg agcacggcac ggatcgcgac gacgaggtca accgcggcgc
     2821 ccggggcggc aacttcgggt ggaacccggt ccccggctat gacgagtcca ctccgatgac
     2881 cgacttcagt ctgcccggca ggcagatcgg ggcacgctgg cgctccggct tcccgacgct
     2941 cgccacctcc ggcgcggtct gggtgcgtgg caagaagtgg ggacgctaca acggcacgct
     3001 ggcggtctgt gcgctgaagg cctccgagct gttgttcatg aagttcgacc gacacggcag
     3061 gttccgctgg atgcgaacgc cgccggccat gcgcggtcgg tacggccggc tgcgttcggt
     3121 cgccctgacg cacaaccacg acctgttggt cacgacctcg aacggtggtg gcggcagcgg
     3181 cggcgaccgg gtcatccgcg tcagcccccg ctgaccgccg gaaaccgctg gtcgagccct
     3241 tcgacaagct caggaaccac ttgtcgagac ccgggctccc gggtcttgac gggtcgaccg
     3301 aaggattggc cgctagaaca agatcgtggc gaaggtgccg gtcgtgacga agccgacgcg
     3361 ttcgtacgta cgacgcgcgg gctcgttgtg ctggttgacg tacaacgaca caaccggtgc
     3421 cacgtcaccc agcgccagct ggcagaccgc ggcgacccgg ctctctgcca gaccctgccc
     3481 acggaaactc gggtcgatcc agacgctctg caggtgcgct gcgtgcggtg tgacggcacc
     3541 gagctcggcc ttgaacacga cccg
//
LOCUS       DATBFZ010000485        16813 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_5438620, whole genome shotgun sequence.
ACCESSION   DATBFZ010000485 DATBFZ010000000
VERSION     DATBFZ010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 16813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16813
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5438620"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1242)
                     /locus_tag="VH419_14725"
     CDS             complement(<1..1242)
                     /locus_tag="VH419_14725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017936373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEY7044920.1"
                     /translation="MSESVNHQVARGDEDDARLADLGYQPQLRRVLGLFANFSVAFTY
                     LSPMVGIYSLFLLGVVAGGPAYIWLTWIPVAGMLLVALVFGELASHYPVAGALYQYSK
                     FSVGPGFGWFVGWFYGFALLITVAAVDTGVVGYVTALTHNWFGWDLDYTDHLTILLIT
                     VLLLAIQTILNITGAKVMGRVAQFGVYVEIVGTLGIALILAIHGFHHGVGFLFSTQDM
                     QNVASNPLGFDFGGSWLAAALIAVLAPVYIFYGFESAGDISEETVDAGRQVPKSMRWA
                     LIWGGVSSFILIGALLLAMPSGDDPVGQTNEGGGVPFILAELPSGLQDFLLLIIIFAF
                     FSCGTSIQGAGSRLAFSYARDGALPRSAWVSKVHARFGTPVNALLTGAVVTALFVLLV
                     FASPDHDVKIGFITYPANVNAL"
     gene            complement(1501..2502)
                     /locus_tag="VH419_14730"
     CDS             complement(1501..2502)
                     /locus_tag="VH419_14730"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY7044921.1"
                     /translation="MIGDALVFDGVAHVFNFESDNAYGPAGTMFSNHLYAFHATLTPD
                     GETVMPPEQFLHKWTIEEIRQMVYDQSGTDMLVAMPLPLTDLYKDGLSPWEECAELAK
                     RDPDKTVFWGTVNPLEGKKALDDVTRQVEEYGARAFKFYNVRYEDAGPVPWRMDDPKV
                     AFPVFERMQELGVNLVGVHKGVPLGPQPIEHTQTWDMDGAAANFPDMNFVIFHVGLPF
                     IDETCWQLIRYPNLYASLAATINFLVRAPRQFAEILGKLLFWCGEDKIVYGSEAPIFH
                     PQWALEAFWNFEIPQDLCEGYGYPQLTEQAKRKILGENLARLHGIDVEATKARMATT"
     gene            complement(2492..2917)
                     /locus_tag="VH419_14735"
     CDS             complement(2492..2917)
                     /locus_tag="VH419_14735"
                     /inference="COORDINATES: protein motif:HMM:NF013999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEY7044922.1"
                     /translation="MSTEVTDIDPSVGAALTDVYDPCCREKGISIIDMGLLHRASIDD
                     GHARVEIVLTSGWCPFASTVLTEIEDVVRQLPGVRTAAVEIVWDEAWTPERLSDTARS
                     KLRFLPPPSGVRNVTSYVAEHRAAAQPTADQSQGASHDR"
     gene            3107..3550
                     /locus_tag="VH419_14740"
     CDS             3107..3550
                     /locus_tag="VH419_14740"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEY7044923.1"
                     /translation="MPLIRDYVAEHERATDLGGDAVRAIEHDELQKARQLIAEMASEL
                     ESHWHGEENGIFAVMRSQKQYADYIAPLVKEHRELAELLTTVDIDDPADQERFRVAVA
                     ELHEHISKEEDGLFPASLTTLRGDEWNVAMKAWQEAHPGETMIPD"
     gene            complement(3583..4272)
                     /locus_tag="VH419_14745"
     CDS             complement(3583..4272)
                     /locus_tag="VH419_14745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044924.1"
                     /translation="MFTSLESQRRIGRIAWYSAWVALVLGQLHALSRFATEDGKEDLD
                     LPLTGAWAEPAGKALRPLLDWAGPDTVYLTYGKVWFPLFLAFTLCAFVVRRRRQAVGF
                     EKWAWRVALLGYVWGAVGTFCDYWTQWADPNAFFDIAFVITIPGLLLTMIGSTMLGTA
                     LLRNGFRPKVSAWLLTAAVPGLLLISEVTSLGNTALPIMFAFGIVGRRIAREPSYAVD
                     VSSTSLLVEHA"
     gene            4422..5540
                     /locus_tag="VH419_14750"
     CDS             4422..5540
                     /locus_tag="VH419_14750"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEY7044925.1"
                     /translation="MKRLPLWLQDAALPAALALIGLVELLVMRPSGWPFGVLVNGLSC
                     LLLVWRRRNALLYATASGVVLLTAPWFGPQLDEPSTPIAILAVITYSLGRFIADLRGM
                     VGLAVIGLSFFGDYLLVDTRDHNISDVVFVTALMAPPYVVGRVARRLAEAAALLERQQ
                     EVVKREAIKAERDRIARELHDVIAHSVSAMVVQTAAAQDIVRSDPVKAEAVLADVAET
                     GRKALVETGRLLHVIRDSDDELGLEPTPGVTNVPELVERFRGSGLVVDLDLDASMRPL
                     PAGVDVSVYRIVQEALTNALKYGSGRTANLKLWSTPTVVSVEASNPSGGGGQVGSGLG
                     LLGMAERVAVLGGSLSHGVDLNGRFVLTATIPLAGGPA"
     gene            5537..6247
                     /locus_tag="VH419_14755"
     CDS             5537..6247
                     /locus_tag="VH419_14755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020386359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY7044926.1"
                     /translation="MTSVVVADDQDLVRTGLEMVLTARGYDVVGLAADGREAVELARR
                     TRPDVVLMDIRMPVLDGIAATAQITAEKLPCRVLVLTTYDVDQYVYDALKAGAAGFLL
                     KATPPDRLADGIALVAAGEALLAPTLTRRLIEEHVRRPPPLNRLPEPLDRLTSREVEV
                     FTLIAHGLSNGEIAHRLVVSEATVKTHVNRLLAKLGLASRVQMVVLGYESGLVRPGDH
                     PHSGVRLVAVDTAAEPAR"
     gene            6374..7975
                     /gene="dacB"
                     /locus_tag="VH419_14760"
     CDS             6374..7975
                     /gene="dacB"
                     /locus_tag="VH419_14760"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012920667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HEY7044927.1"
                     /translation="MSGRFTRRGVTSTATTLGASVIAVLALTGPSASVAPASASRAAP
                     AADTLASQIDALLTDPRYDGSQVGVVVRDADTGATLYDHNGDERLIPASNTKLFTSTA
                     ALDLLGSDYRFRTTVSTSGGQRGSTVKGDLYLTGGGDPTTLASDYGDLAAQLRDRGVR
                     TVRGNLVADDSFFDHQRLGAFWSWDDEPFYYSAQISGLTVAPNTDYDSGTVIVETRAG
                     SKVGRPAQLTLVPKTHYVHVVNDAVTGAAGSDNTISVEREHGNNIIHVTGSFPIDETV
                     EQDWSTVWEPTGYAADVFKRALRDHGIDLRGRVRTGVTPDATTTLATHESMTLGDMLI
                     PWLKLSNNMHAEHIVKTIGAEVEGDGSWDAGLDAIESDLPSLGVDTDVMRMVDGSGLA
                     RADFIPPDQITNLLVAARSKPWFDQWYNALPIAGNPDRFVGGTLRSRMVGTPAANNLH
                     GKTGSLTGVTALSGYVTDADGDPLVFSIVSNNYLSSPRSVEDALGVLLASYSADTGAV
                     APNVRALRPTHRLPADIECSWAKAC"
     gene            complement(8048..8611)
                     /locus_tag="VH419_14765"
     CDS             complement(8048..8611)
                     /locus_tag="VH419_14765"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY7044928.1"
                     /translation="MTAELHVLCAGYAHDRVASTVTLIRDEGRAVIVDPGMVSDRRNI
                     LEPLASLGVDPSEVGDVVFSHHHPDHTLNAALFERARFHDFWAIYEADNWTDRPADGF
                     QLSASVRLMATPGHTAEDITTLVETSEGLVACTHLWWSEDGPAADPLAEDQAALERSR
                     ARLLELEPLLIVPGHGAPFPAHRSAAG"
     gene            complement(8733..9143)
                     /locus_tag="VH419_14770"
     CDS             complement(8733..9143)
                     /locus_tag="VH419_14770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044929.1"
                     /translation="MTALTPYLHFPGTARDALMFYGKVFDCAVQMHTFAEFNRTDGPA
                     DAIAHGYLAEGPVSLFVADVTGDEPPFRCEGMMLSLLGTAAPSTLQTWFTRLSEGGRI
                     VDDLQVRPWGASDGQVIDRYGVHWLIGFEEDAGD"
     gene            9282..10592
                     /locus_tag="VH419_14775"
     CDS             9282..10592
                     /locus_tag="VH419_14775"
                     /inference="COORDINATES: protein motif:HMM:NF013918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin reductase family protein"
                     /protein_id="HEY7044930.1"
                     /translation="MTSVVRGIAWLGIFVGICVAPLVFALMSTNQPGQGFWTDFSVAL
                     GFVGLALMGIEFALVARVKAVAEPFGTDGVIDFHRQIGYAGLIFIVIHVALSADWSQA
                     NPLTPGNTPARVWFGGIAAAALVVLVASSVWRRQLRLSYEVWQLLHTVLAVIAVVAAL
                     VHVLLVDYYVDNVWKQVLWSIMTAAFVGLLVWVRVLRPLRMRRRPWTVERIRPERDSS
                     TVLTVLPTGHEGMRFAPGQFGWITVDRSPFSVTSHPFSFCSSAEKDGSLEFAIKAAGD
                     FSSGVATVEPGTKVFVDGPHGVFSIDQYEGAGFCLIAGGVGIAPALSMLETLADRGDV
                     RPVVVFAGNRNWESIILRERIDELTTRLNVTVVHVLEEPPPDWDGEQGYIDVDIFTRH
                     LPAGFRRFQFFACGPNPMLAAVGTALVGIGVPADRIHTESFDWV"
     gene            complement(10753..12111)
                     /locus_tag="VH419_14780"
     CDS             complement(10753..12111)
                     /locus_tag="VH419_14780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017933653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEY7044931.1"
                     /translation="MTDLASLAAAVRDGMPRARTELGRLVALRSVADPDIEPAEECVE
                     AASAVADLLREARVDGVHAIETADGSLAVVGHTPAPAGAPTVLLYAHYDVQPAGELSE
                     WASSPWELTERDERWYGRGAADCKGNLVMLLCALRALPQPWPVGVRVVCEGSEEMSTG
                     GLEELVRTQPSLFAADVMLIADSGNVELGTPTVTTSLRGTGSVVVTVSTLDGPVHSGM
                     YGGAAPDALAALIAILATLRDADGSTTIRGLDASGTWTGADYPIDRFRRDAGVLDGVS
                     VLGGSTVADALWARPAVAVLAVDAPSVAGATAAIQHTARALVNLRVPPGYDAAVAQDL
                     LVDHLRTAAPWGVHVDVVPRSLGEPFAARTDGPGFAALSAAMEQSFGRPLTTAGQGGA
                     IPLCNTLQDAHPDAEILLIGVEEPGCRIHAPNESVHPAELERTAVGVALFLSSLAGST
                     SG"
     gene            complement(12108..12836)
                     /locus_tag="VH419_14785"
     CDS             complement(12108..12836)
                     /locus_tag="VH419_14785"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEY7044932.1"
                     /translation="MSELKATEQWYSDRMKQAMSLARWGSYGVPVLVFPTAGGDAEEV
                     ERHHLVGHLWPLVEAGRVKVYSCDSVAGAAMAKGDGSADYRCWLFNQFQQAVANEVVP
                     AIHADSAGPHDVIVAGASIGAFNALAMISRFPQLFRAAICMSGTYNLERMVGGFTEDL
                     FFSSPIHFLPGLDGPILDLLRQRFVYIASGSGAWEDVGESWRVAEVLGAKGVPNRVDD
                     WGPEYEHDWPTWWQMLPTYVEQAL"
     gene            complement(12855..14030)
                     /locus_tag="VH419_14790"
     CDS             complement(12855..14030)
                     /locus_tag="VH419_14790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013866130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEY7044933.1"
                     /translation="MTAVPTARKGRQRLAINRLMDRRPLDAATIDRFIDRYGAPVVEG
                     ARTTFLWRGDADEVMVRHRVMGLTDPLRLRRIKDTDLWYVTTEMPEGSRVEYQFEIVR
                     GDQHESFVNDPLNPRLAHGPFGASSVCAATGYVVPDWTLPDPEARPGELVEHSLASKA
                     LRRQVPFLVYLPARFRRVIRYPLVVVHDGTDYLDYTGAKTVLDNLIHRNEVAEMIVAF
                     VPPGNRLVEYANHAPHARFVARELVPYLTEGYPLIDTPDGRTLMGSSFGGIASLSTAV
                     RYPGQFGSLFLQSASLVFTDIGLDHGGGPAFDPVVKFVNAFRARPKRVVERIYMTCGM
                     YEPLITPNRSMVSTFRETGTEVRYAEARDGHNWENWRDRLRDGLAWLHPGEQKFVYE"
     gene            complement(14027..14950)
                     /locus_tag="VH419_14795"
     CDS             complement(14027..14950)
                     /locus_tag="VH419_14795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044934.1"
                     /translation="MRTILLGPQRFLTTAGAAVHSLSVDGHVAIVTAGWRDREDDDAE
                     LDSVLDGRSRNLGLFGRLTDVLDTDTRFAAAALEHRDAMDDLVAVYSLRLQRTLDSVY
                     AAQRRTVRADIADAALADGVRAVRAVDSWYLDTVDQLYGELELASPVAESEPVQRHRA
                     EVAEILESAGVLAIAGGHVGVLLRCLKLFAVLPPVDMPVVAWSAGAMALTDRVVLYND
                     NGPEGRQGAELWDRGLGRVHGVVAMPHARRRLRMDDPMTLRVLVRRFVDASCLLLDDG
                     TRVDMGADGEIPDGARVLSDYGSVGTVGGLP"
     gene            complement(14947..16164)
                     /locus_tag="VH419_14800"
     CDS             complement(14947..16164)
                     /locus_tag="VH419_14800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013866132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEY7044935.1"
                     /translation="MNIVFVEPFFPHNQRRFVHALAGVGANVIGVGEYSEHELGDELR
                     GQMGAYYKVSNVTDIGQLNAATEWAQNLAWIDRLESTIEAHQMAAAQVREARGIPGTS
                     VRTTWLCRDKPSMKEALRQAGVPTAASTAAHNADEVIDFANRIGFPLILKPRAGAGAQ
                     GTTRVDSMAELQAALASFGSADSIAVEEFVEGHEGFYDTITIDGHIVHDWMTHYYPNV
                     LEAMRHRWISPQFVTTNRIEDSLFYSEVRELGSRVIEALGIPTSATHMEWFYGPKGLR
                     FSEIGCRPPGVGAWDLYSAANDVDVYREWAHAIVHGSPENKLSRQYSAGIVALRPEQD
                     GQIRGYSGLDEINERHGRWIIDAHLPNPGTPTQPVEAGYMANAYIRMRHPDYDTLRGM
                     LDEAGRTVRVHAG"
     gene            16201..>16813
                     /locus_tag="VH419_14805"
     CDS             16201..>16813
                     /locus_tag="VH419_14805"
                     /inference="COORDINATES: protein motif:HMM:NF018154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY7044936.1"
                     /translation="MEHKPVVYLITGPMAAGKSTVARLLAARFERGVHLEGDLFRRSI
                     VSGREEMTPHPSPAALEQLGLRYRLAAAAADAYVEAGFSVALEDVAAGGWLGDYRTMI
                     RSRPCHVVVLLPSVEVITAREAGRDHKGYSTWTIEQLYEGFAGSTPRVGIWLDTTHLT
                     PEQTVDEILSQTDSTRSPIVVSDYDDRWPDAFEQIAKPVRAAVA"
BASE COUNT         2751 a         5669 c         5692 g         2701 t
ORIGIN      
        1 gagcgcgttg acgttcgcgg gataggtgat gaagccgatc ttgacgtcat ggtcgggaga
       61 ggcgaagacc agcagcacga acagcgcggt cacgacggcg ccggtgagca aggcgttcac
      121 cggcgtaccg aatcgagcgt gcaccttcga cacccacgcg gacctcggca acgcgccgtc
      181 gcgggcgtag gagaacgcga gccgactgcc cgctccctgg atcgaggtgc cgcacgagaa
      241 gaacgcgaag atgatgatca gcagcaagaa gtcctgcagc ccgctgggca gctccgccag
      301 gatgaacggc acccctccac cctcgttcgt ctggccgaca gggtcgtcac ctgacggcat
      361 ggcaagcagc agcgccccga tgaggatgaa cgacgagaca ccgccccaga tcagcgccca
      421 ccgcatcgac ttggggactt ggcgaccagc gtcaaccgtc tcctccgaga tgtcgccggc
      481 ggactcgaaa ccgtagaaga tgtagacggg agcgagcacc gcgatcagcg cggcggccag
      541 ccagcttcca ccgaagtcga atcccaaggg gttgctcgcg acgttctgca tgtcctgcgt
      601 cgagaagagg aagcccacgc cgtggtggaa cccgtggatg gcgaggatca gcgcgatacc
      661 gagggtgccg acgatctcga cgtacacgcc gaactgcgca acgcgaccca tgaccttggc
      721 gccggtgatg ttgaggatgg tctggatcgc cagcagcagg accgtgatca gcaggatcgt
      781 gaggtgatcg gtgtagtcga ggtcccagcc gaaccagttg tgcgtcagtg cggtcacgta
      841 gccgacgacc ccggtgtcga cggcggcgac ggtgatcagc aacgcgaagc cgtagaacca
      901 tcccacgaac cagccgaagc ctggacccac ggagaacttg ctgtactggt acagcgctcc
      961 cgccaccgga tagtggctcg cgagctcgcc gaacaccagc gcgaccagca gcatgccggc
     1021 gaccgggatc caggtgagcc agatgtacgc cggtccgccc gcgacgactc ccaggaggaa
     1081 cagcgagtag atgccgacca tcggcgacag gtaggtgaac gcgacggaga agttcgcgaa
     1141 gagcccgagg accctgcgca gctgcggttg gtatcccagg tcggcgagtc gggcgtcgtc
     1201 ctcgtcgccc ctggcaacct gatggttgac ggattcgctc atgggggtct ccactcctcg
     1261 actgcggcgc ggtgccgctg tagacgacgt gtcccccgtc cagcctgtgt cgccggtccg
     1321 aggaccggtc gccatcgccg aaggtaccaa gaccggcagt gggcaggctc cggttacggc
     1381 gccgggtcgc ccggcgtgtc gtgtcgcgag tgagccctcg cgaatgcggg aaacatgtgc
     1441 gcgagggcag tggttcagca ggacctttcc cgcaattcgc gggaagccgg gatggccgga
     1501 tcaggtcgtg gccatgcgtg ccttcgtcgc ctcgacgtcg atgccgtgca gtcgggcgag
     1561 gttctcgccg aggatcttgc gcttcgcctg ctcggtgagc tgggggtagc cgtagccttc
     1621 gcacaggtcc tgcgggatct cgaagttcca gaacgcctcg agcgcccact gcggatggaa
     1681 gatcggtgct tccgagccgt acacgatctt gtcctcgccg caccagaaca gcagcttgcc
     1741 gaggatctcg gcgaactgcc gcggcgcgcg aacgaggaag ttgatggtgg ccgccagcga
     1801 cgcgtacagg ttcggatagc ggatcagctg ccagcaggtc tcgtcgatga acggcagccc
     1861 gacgtggaag atgacgaagt tcatgtcggg gaagttcgcg gccgcaccgt ccatgtccca
     1921 ggtctgcgtg tgctcgatcg gctgcggccc gagcggcaca cccttgtgaa cgccgaccag
     1981 gttgaccccg agttcctgca tccgctcgaa gaccggaaac gccactttcg ggtcgtccat
     2041 ccgccacggc accgggccgg catcctcgta gcgcacgttg tagaacttga acgctcttgc
     2101 gccgtactcc tccacctggc gggtcacgtc gtcgagcgcc ttcttgccct cgagcggatt
     2161 caccgtcccc cagaacaccg tcttgtcagg atcacgcttc gccagctccg cgcactcctc
     2221 ccagggagac agaccatcct tgtagagatc ggtcagcggc agcggcatgg cgacgagcat
     2281 gtccgtgccg gactggtcgt agaccatctg cctgatctct tcgatggtcc acttgtgcag
     2341 gaactgctcg ggcggcatga cggtctcgcc gtctggcgtg agggtcgcgt ggaacgcgta
     2401 caagtggttg gagaacatcg tcccggccgg cccgtacgcg ttgtcggact cgaagttgaa
     2461 tacgtgcgct acgccgtcga agaccagcgc gtcaccgatc atgagaggct ccctggctct
     2521 gatctgctgt tggttgcgcc gccgcgcgat gctcggcgac atatgaagtc acgttcctca
     2581 ctcctgaggg aggcgggagg aacctgagct tggagcgggc ggtgtcggac agccgctccg
     2641 gagtccaggc ttcgtcccac acgatctcga ccgcggcggt acgcactcct ggcagctgtc
     2701 gcacgacgtc ttcgatctcg gtgagcacgg tgctcgcgaa tgggcaccag cccgacgtca
     2761 gcacgatctc gacgcgtgcg tgaccgtcat cgatcgaggc gcggtgcagc agtcccatgt
     2821 cgatgatcga gatgcccttc tcacggcagc aggggtcgta cacgtccgtg agagcggcgc
     2881 ctaccgacgg atcgatgtct gtgacttcgg tcgacactcc ttgtccctcc caggccccgt
     2941 ttctgggttc agtgtcgtcc cggcccggcg gtttgggtag cccttggcgt ctccttcctg
     3001 catccctgcg ggcggaaggg cgggtacgcg caattcgtgc tgaactgagg acagcacgat
     3061 cggggtggga aggacggaca tgtgccacta ctgcggatgc cgacagatgc cgctgatccg
     3121 cgactacgtg gccgagcacg agcgggcgac cgaccttggt ggtgatgccg tccgcgcaat
     3181 cgagcacgac gagctccaga aggcccgcca gctgatcgcc gagatggcgt ccgagctgga
     3241 gtcgcactgg cacggcgagg agaacggcat cttcgcggtg atgcggagcc agaagcagta
     3301 cgccgactac atcgcgccac tcgtcaagga gcaccgtgaa ctcgcggagc tgctcaccac
     3361 cgtcgacatc gacgacccgg ccgaccagga acggttccgt gtcgctgtcg cagagctgca
     3421 cgagcacatc agcaaggagg aggacggact cttcccggcg tcgctgacga cgctgcgtgg
     3481 cgacgaatgg aacgtggcga tgaaggcgtg gcaggaggcc caccccggcg agacgatgat
     3541 cccggactga cgacacgcct cgccggggag agctgcacca gctcaggcat gctcgaccag
     3601 caggctcgtc gacgagacgt ccacggcgta cgacggttcc cgagcgatac gccgtcccac
     3661 gatgccgaac gcgaacatga tcggaagcgc cgtgttgccc agtgaggtca cctcactgat
     3721 caacaacagc ccggggacag ccgccgtcag cagccaggcc gacaccttcg gccggaagcc
     3781 gttgcggagc agggctgtac ccagcatcgt cgagccgatc atcgtgagca gcagtcccgg
     3841 gatggtgatg acgaacgcga tgtcgaagaa cgcgttgggg tcggcccatt gggtccagta
     3901 gtcgcagaac gtcccaacag ccccccagac gtagccgagc agggccaccc gccacgccca
     3961 cttctcgaag ccgactgctt ggcggcgacg gcgaaccacg aaagcgcaca gcgtgaacgc
     4021 gaggaacagc gggaaccaga ccttgccgta ggtcaggtag accgtgtcgg ggccggccca
     4081 gtcgagcagt ggccgtagcg ccttccccgc cggctctgcc cacgccccgg tcagaggaag
     4141 atcgaggtcc tccttgccgt cttcggtggc aaaccgcgac agtgcgtgca gctgtccgag
     4201 gacgagcgcc acccatgcgc tgtaccaggc gatgcggccg atccgccgct ggctctccaa
     4261 cgatgtgaac acgtgagtct ccttggtcgt gtcgtcgtgc ggacgtgttg ggtcgtgatg
     4321 aggctaggca gcgtgagcag tgctcgtcgt cagccgacgg ttcggaacgt gtcaacccaa
     4381 aggtggacgt cgaacgtcgt ggagatcgtt agcctcggac tgtgaaacgc ctgccgctgt
     4441 ggctgcaaga cgctgcgctt ccggctgccc tcgcactgat cgggctcgtc gagctcctcg
     4501 tcatgcggcc gtcggggtgg ccattcggtg tactggtcaa cggtctctcg tgcctcttgc
     4561 tggtgtggcg acgccgcaac gctctcctgt acgcgacggc atccggtgtg gtgctcctga
     4621 ccgcaccatg gttcggccca cagctggacg agccctcgac accgattgcg atccttgccg
     4681 tcatcacgta ctcgctcggg aggttcatcg ccgatcttcg gggcatggtc ggactcgcgg
     4741 tcatcgggct ttcgttcttc ggcgactacc tcctcgtcga cacccgcgac cacaacatct
     4801 ccgacgtggt gttcgtgacc gcgctgatgg cgccgccgta tgtcgtcggc cgggtcgccc
     4861 gcaggctcgc tgaagcggca gcgctcctcg aacggcagca ggaggtcgtc aagcgtgagg
     4921 cgatcaaggc cgagcgggac cggatcgcgc gcgagctgca cgacgtgatc gcgcattcgg
     4981 tgagcgcgat ggtggtgcag accgcggcag cgcaggacat cgtccgttcg gacccggtca
     5041 aggccgaggc cgttctcgcc gacgttgcgg agacgggccg caaggcgctg gtcgagaccg
     5101 gtcggctgct gcacgtgatc cgcgactccg acgacgagct gggcctcgag ccgacgccgg
     5161 gcgtcaccaa cgtccctgag ctggtcgaac ggttccgggg cagcggcctc gtcgtcgacc
     5221 tggacctcga cgcatcgatg cggccgttgc ccgccggcgt cgacgtgtcg gtctaccgca
     5281 tcgtccaaga ggcgctgacg aacgcgctga agtacggatc cgggcggacg gcgaacctga
     5341 agctctggtc gacgccgacc gtggtctccg tcgaagcatc caacccgtcc ggcggcgggg
     5401 gccaggtcgg cagcggcctg gggttgctcg ggatggccga acgcgtcgcc gtgctcggtg
     5461 gcagcctgtc tcacggcgtc gacctgaacg gacggttcgt gctcaccgcg acgattccgc
     5521 tcgccggagg tccggcatga cgagcgtggt ggtggccgac gaccaggacc tcgtccggac
     5581 gggccttgag atggtgttga cggcgcgcgg gtatgacgtc gtcggtctcg cggcggacgg
     5641 acgggaggcc gtcgagctgg ctcgccgcac tcgacccgac gtggtcttga tggacatccg
     5701 gatgcccgtc ctggacggca tcgccgcgac cgcccagatc accgcggaga agctgccttg
     5761 ccgcgtgctc gtcctcacga cctacgacgt cgaccagtac gtctacgacg cgctgaaggc
     5821 cggggcggcc ggcttcctgc tcaaggccac gccgccggac cggcttgccg acggaatcgc
     5881 gctggtggcg gcgggcgagg cactgctcgc accgacgctg acgcgccgac tgatcgagga
     5941 gcatgtacga cgtccgccgc cactgaaccg gctccccgag ccgttggatc gactgaccag
     6001 ccgcgaggtc gaggtgttca ccctgatcgc gcacggtctg tcgaacggtg agatcgccca
     6061 ccgcctcgtc gtgtccgagg cgacagtgaa gactcacgtc aaccgcctgc tggccaagct
     6121 cggcctcgcc agccgggtgc agatggtcgt cctgggctac gagtccggcc tcgtccgccc
     6181 tggtgaccac ccgcacagcg gcgttcgcct cgtcgctgtc gacaccgctg ctgaacctgc
     6241 ccggtagcga cgcgcccttc gcaggtcttc cgctgcaccg tctcgatggg cttggatgcc
     6301 gtggggcgcc tttcgcccca cgaaggccgt tgcgccagat caccggcgta cccaccagga
     6361 aaggtctcaa cagatgtcag gaaggttcac ccgtcgcggc gtcacctcga ccgcaacgac
     6421 tctcggggcg tccgtcatcg cggttctcgc cctcaccggc ccatcggcct cggtcgcacc
     6481 ggcgtccgcc agccgcgcgg cccctgcagc ggacacgctc gcaagccaga tcgacgccct
     6541 gctcaccgac ccgcggtacg acggctcaca ggtcggggtc gtcgtccggg acgccgacac
     6601 cggcgccacc ctgtacgacc acaacggtga cgagcggctg atcccggcgt ccaacaccaa
     6661 gctgttcacg tcgacggccg cgctcgacct gctcggttcc gactatcggt tccgcaccac
     6721 ggtgtcgacg agcggagggc agcgcggctc taccgttaag ggcgacctct acctgaccgg
     6781 cggcggcgac ccgacgacgc tggccagcga ctacggcgac ctggcggccc agctgcgcga
     6841 ccggggggtg cgcacggtcc gcgggaatct cgtggcggac gacagcttct tcgaccatca
     6901 gcggctcggc gcattctggt cgtgggacga cgagccgttc tactactcgg cacagatctc
     6961 cggtctcacc gtcgcgccga acaccgacta cgactccggc accgtgatcg tcgagactcg
     7021 cgccggctcg aaggtcggcc ggcccgcaca gctcacgctc gtgccgaaaa cccactacgt
     7081 gcacgtggtc aacgacgccg tcaccggcgc cgccggctcg gacaacacga tctccgtgga
     7141 acgggagcac ggcaacaaca tcatccacgt gaccggcagt ttcccgatcg acgagacggt
     7201 cgagcaggac tggtcaacgg tgtgggagcc gaccgggtac gccgccgacg tgttcaagcg
     7261 cgcgttgcgc gaccacggca tcgacctgcg cggccgggtg cgtaccgggg tgacaccgga
     7321 cgccacgacg acgctcgcga cccacgagtc gatgacgctc ggtgacatgt tgatcccttg
     7381 gctcaagctg tccaacaaca tgcacgcgga gcacatcgtg aagaccatcg gggccgaggt
     7441 cgagggcgat ggcagctggg acgccgggct ggacgcgatc gagagcgacc tgccgagcct
     7501 cggcgtcgac acggacgtca tgcgcatggt cgacggctcc ggtctcgcca gggcggactt
     7561 catccctccc gaccagatca ccaacctgct cgtcgccgcc cgcagcaagc cgtggttcga
     7621 ccagtggtac aacgcgctgc cgatcgccgg caacccggac aggttcgtcg gcggtacgct
     7681 gcggtcccgc atggtcggaa ccccggccgc gaacaacctg cacggcaaga cgggttcgct
     7741 caccggggtc accgcactgt ccggctacgt caccgatgcc gacggcgacc cgctggtgtt
     7801 ctcgatcgtc tcgaacaact acctgagcag ccctcgcagc gtcgaggacg cgctcggcgt
     7861 actgctggcc tcgtacagtg cggacacggg agcggtggca cccaacgttc gcgcgctgcg
     7921 gccgacgcac cggctgcccg ccgacatcga gtgctcatgg gcgaaggcct gctaggtcgc
     7981 tagtgtcccg gagcctcatg cgggatccgc cgcaccagtg gcggatcccg cgtcggctcg
     8041 tcctcgatca gccagcagcg gagcggtggg ccgggaacgg tgcgccgtgg cctggcacga
     8101 tcagcaacgg ctccagctcc agcagcctgg ctcgcgagcg ttcgagcgca gcttggtctt
     8161 ccgccagcgg atccgctgcg gggccatcct ccgaccacca aaggtgcgtg caggccacca
     8221 gcccttccga cgtctcgacg agcgtcgtga tgtcttcggc cgtgtggccg ggtgtcgcca
     8281 tcaaccgcac tgaagcagac agctggaatc cgtcggccgg cctgtcggtc cagttgtctg
     8341 cctcgtagat cgcccagaag tcgtgaaacc gggcccgctc gaacagtgcc gcgttcaacg
     8401 tgtggtctgg gtggtggtgg ctgaacacca cgtcgcccac ctcagacggg tcaacaccga
     8461 ggctcgccag cggttcgagg atgttgcggc ggtcggacac cattccggga tcgacgatga
     8521 ctgctcggcc ttcatcgcgt atcaacgtca ccgtgctggc cacccggtcg tgggcgtagc
     8581 cggcgcagag tacatgcagt tcggccgtca cgccgcaatc ctggcacgtc gctctcggcg
     8641 atcacaccag atggtctggt ccccgcaccg gtcgacgtcg cctcagactg ggactggcac
     8701 ctctgtcctt tcgcccgtcc tggtcccgga ggtcagtcgc ctgcgtcctc ctcgaatccg
     8761 atcagccaat ggacgccgta gcggtcgatg acctgtccgt cggatgcccc ccacggccgg
     8821 acctgcaggt cgtccacgat ccgaccacct tccgagagcc gggtgaacca ggtctgcagg
     8881 gtggacgggg cggcggtccc gagcaacgac agcatcatgc cttcgcatcg gaatggtggc
     8941 tcgtcgccgg tcacgtcggc gacgaagagg ctgaccggcc cttcggcgag atagccgtgg
     9001 gcgatggcat ccgccggacc gtcggtcctg ttgaactcgg cgaaggtgtg catctgtacg
     9061 gcgcagtcga agactttgcc gtagaacatc agggcgtcac gagcagtgcc ggggaaatgg
     9121 aggtagggcg tcaatgcggt catgccaggg gatgttaggc gaaattccca gccgaacacg
     9181 ccaacaggga tggagaccta tcagggtcgc ggcgcgggca tagactgtcg gcgttttgcg
     9241 gggtggaatg ccccgccgcc aaccgtgaag cgaggcgcgc catgacgtcg gtcgtgcgcg
     9301 gaatcgcgtg gctcgggatc ttcgtcggga tctgcgtggc gccgctggtc ttcgcgctga
     9361 tgagcacgaa ccagccgggg caaggtttct ggaccgactt ctccgtggct cttggcttcg
     9421 tcggcttggc cctcatgggg atcgagtttg cgctggtcgc gcgggtcaag gccgtcgcgg
     9481 agccgttcgg cacggacggg gtgatcgatt tccatcggca gatcggttat gcgggcctga
     9541 tcttcatcgt gatccacgtg gcactgtccg ccgactggag ccaggccaat cccctcacgc
     9601 cgggcaacac gcccgcccgg gtctggttcg gcggcatcgc ggcagccgcc ttggtggtcc
     9661 tggtggccag ctcggtctgg cgtcgtcagc tgcgactgtc ctatgaggtg tggcagctcc
     9721 tgcacaccgt gctggcggtg atcgctgtgg tggccgcctt ggttcacgtg ctgttggtgg
     9781 actactacgt cgacaacgtc tggaagcagg tgctgtggtc gatcatgacg gcggctttcg
     9841 tcgggctgct cgtgtgggtg cgcgtgcttc gtccactgcg gatgcgtcga cggccgtgga
     9901 cggtggaacg gatcaggccg gagcgtgaca gctcgaccgt cctcacggtg ctcccgaccg
     9961 gccacgaggg gatgcggttc gcacccggcc agttcggctg gatcacggtc gacagatcgc
    10021 cgttctcggt cacctcccac ccgttctcgt tctgctcgag cgccgagaag gatggcagcc
    10081 tcgagttcgc catcaaggcg gctggggact tctcgtccgg cgttgccacc gtcgagccgg
    10141 ggacgaaggt cttcgtggac gggccgcacg gggtgttctc gatcgaccag tacgagggcg
    10201 ccggtttctg cctgatcgcc ggtggagtcg ggatcgcgcc cgccctgagc atgctggaga
    10261 cactggccga ccgtggcgac gttcggcctg tcgtcgtgtt cgccggcaac cgcaactggg
    10321 agagcatcat cttgcgcgag cgcatcgacg agctcaccac tcggctgaac gtgacggtgg
    10381 tgcacgtcct cgaggagccg ccgcccgact gggacgggga gcaggggtac atcgacgtcg
    10441 acatcttcac ccgccacctg cccgccggct tccggcggtt ccagttcttc gcctgcggcc
    10501 ccaacccgat gctcgccgcc gtggggaccg cactcgtcgg aatcggcgtg ccggccgatc
    10561 gcatccacac cgaaagcttc gactgggtct gaagccggct gcccgagaag gagggaccgt
    10621 gcgccacgtc tacctggccc gtatcgcgct ggccacgagt gtcctcctgc tgctcgcctg
    10681 cttggccttc gccgccatcc agaactgaac gacctccggc cgagcatccg gactggtcgg
    10741 gacccgccaa actcaaccgg atgtcgagcc ggccaggctg ctgaggaaca gggcaacgcc
    10801 gaccgccgtg cgttcgagct cggcggggtg gacgctttcg ttcggagcat ggatccggca
    10861 gccgggctcc tcgaccccga tgagcaggat ctcggcatcc ggatgggcgt cctgcagtgt
    10921 gttgcagagc ggaatggcac cgccttggcc cgcggtggtc aggggccgcc cgaacgactg
    10981 ctccatcgcc gcggacagtg ccgcgaagcc cgggccgtcg gtgcgggcgg cgaacggttc
    11041 acccagtgaa cgcgggacga cgtcgacgtg cacgccccac ggagctgccg tcctcaggtg
    11101 gtcgaccagc aggtcttggg ccaccgcggc gtcgtagccg ggcgggacgc ggaggttcac
    11161 cagcgcccgg gcggtgtgct ggatggccgc tgtggcgccg gccaccgagg gagcgtccac
    11221 ggcgagaacg gccaccgcgg ggcgggccca gagcgcgtcg gcgacggtcg acccgccgag
    11281 tacggagacg ccgtcgagca caccggcatc acgccggaac cggtcgatcg gatagtcggc
    11341 gcccgtccac gtcccggaag cgtccagacc cctgatcgtc gtgctcccgt cggcgtctcg
    11401 cagggtcgca aggatcgcga tcagcgcggc cagcgcatcc ggggcggcgc cgccgtacat
    11461 gccggagtgg accggcccgt ccagcgtgct cacggtgacg acgacactgc ccgtcccgcg
    11521 caggctggtg gtgacggttg gcgtgccgag ctcgacgttt cccgagtcgg caatcagcat
    11581 gacgtcggca gcgaacaacg agggctgcgt ccgcaccagc tcctcgagac cgccggtcga
    11641 catttcctcc gagccctcgc agaccacccg tactccgaca ggccagggct gcggaagtgc
    11701 ccgcagcgcg cacagcagca tgaccaggtt gcccttgcag tcggccgcac cacggccgta
    11761 ccacctctcg tcccgctcgg tgagctccca gggcgaggac gcccactcgg acagctcgcc
    11821 ggccggctgg acgtcgtagt gcgcgtagag caggacggtc ggagcaccgg ccggcgccgg
    11881 cgtgtgaccg acgacggcga ggctgccgtc agcagtttcg atcgcgtgta cgccgtccac
    11941 gcgggcctcg cggagcagat cggcgaccgc cgaagccgcc tcgacgcact cctcggccgg
    12001 ctcgatgtcc gggtcggcga cggagcggag ggcgacgagc cgcccgagtt cggtccgggc
    12061 gcgtggcatc ccgtcgcgaa ccgctgccgc gagagaggcc aggtcggtca caaggcctgc
    12121 tcgacgtacg tcggaagcat ctgccaccac gtcggccagt cgtgctcgta ctccgggccc
    12181 cagtcgtcga cacgattggg aactcccttg gcgccgagga cttcggcaac tcgccacgac
    12241 tcgcccacgt cctcccacgc gcccgagccc gaggcgatgt agacgaagcg ctggcggagg
    12301 aggtccagga tggggccgtc gaggccgggc aggaagtgga tcggcgagga gaagaacagg
    12361 tcctcggtga agccgccgac catgcgctcg aggttgtagg tgccactcat gcagatggcg
    12421 gcgcggaaca gttgcgggaa gcgggagatc atggccagcg cgttgaacgc gccgatcgac
    12481 gccccggcga cgatgacgtc gtgtggcccg gccgagtcgg cgtggatcgc gggcacgacc
    12541 tcgttcgcaa cggcctgctg gaactggttg aacagccagc accggtaatc cgccgaaccg
    12601 tcacctttgg ccatggcggc cccggcgacg ctgtcgcagg agtagacctt gacccgaccg
    12661 gcctcgacca acggccacag gtgcccgacg aggtggtgcc gctccacctc ctcggcatca
    12721 ccgccagccg tggggaagac caggacgggc acgccgtagc tgccccatcg cgcgagcgac
    12781 atcgcctgct tcattcggtc cgagtaccac tgttcggtgg ccttgagctc gctcatcgca
    12841 ctcctcgtcc ctgctcactc gtagacgaac ttctgctcac cggggtgcag ccaggcgagc
    12901 ccgtcgcgca gccgatcccg ccagttctcc caattgtggc cgtcccgcgc ctcggcgtac
    12961 cgcacctcgg tccccgtctc ccggaacgtc gacaccatcg accggttcgg cgtgatcagc
    13021 ggctcataca tgccgcaggt catgtagatg cgttcgacga cccgcttcgg ccttgcccgg
    13081 aaggcgttga cgaacttgac gacgggatcg aaggccggtc ctcctccgtg gtcgaggccg
    13141 atgtcggtga agacgagcga cgccgactgc aggaagagcg agccgaactg cccggggtag
    13201 cgcacggccg tcgacagcga cgcgatgccg ccgaagctcg agcccatcag cgtgcgcccg
    13261 tccggggtgt cgatgagcgg atagccctcg gtgaggtacg gcaccagttc gcgggcgacg
    13321 aaccgcgcat gcggtgcatg gttcgcgtac tcgaccagcc ggttgccggg cggcacgaac
    13381 gccacgatca tctccgcgac ctcgttgcgg tggatgaggt tgtcgagcac cgtcttcgcg
    13441 ccggtgtagt cgaggtagtc cgtcccgtcg tggaccacca ccagcgggta gcggatgacc
    13501 cggcggaacc gcgccggcag gtagacgagg aacggcacct gacggcgcag cgccttgctg
    13561 gccagcgagt gctcgacgag ctcgcccgga cgtgcctcgg gatcaggcag cgtccagtcc
    13621 ggtacgacgt agccggtggc cgcgcacacc gagctggcgc cgaacggtcc gtgcgcgagc
    13681 cgggggttga gcgggtcgtt gacaaaggac tcgtgctggt cgccacggac gatctcgaac
    13741 tggtactcca cccgagaccc ctcgggcatc tcggtggtga cgtaccagag gtcggtgtcc
    13801 ttgatccgtc gaagtctgag cgggtcggtc aggcccatca cccggtgccg gaccatcacc
    13861 tcgtcggcgt cgccgcgcca caggaacgtc gtacgggcgc cttcgacgac gggggcaccg
    13921 taacggtcga tgaaccggtc gatggtcgcc gcgtccaacg gccgtcggtc catcaaccgg
    13981 ttgatcgcga gccgctggcg ccccttccgc gcggtgggca cagccgtcat ggcagaccgc
    14041 ccacggtgcc gacagacccg tagtcgctga gcacccgcgc gccgtccggg atctctccgt
    14101 cggctcccat gtcgacccgg gtgccgtcgt cgagcagcag gcagctggcg tcgacgaacc
    14161 ggcggaccag gacccgaaga gtcatggggt cgtccatgcg cagccggcga cgcgcgtgcg
    14221 gcatcgcgac gacgccgtgg acgcggccga gtccgcgatc ccagagctcc gcaccctggc
    14281 ggccctctgg tccgttgtcg ttgtacaaga cgacccggtc ggtcagcgcc atcgctcccg
    14341 ccgaccacgc gacgacaggc atgtcgaccg gtggcagaac ggcgaagagc ttgagacagc
    14401 gcagcaggac gccgacatgg ccgccggcga tggcgagcac accggccgac tccaggatct
    14461 cggcaacctc ggcgcgatgc cgctggacag gctcgctctc ggcgaccggc gacgccagct
    14521 cgagctcgcc gtagagctga tcgacggtgt cgaggtacca cgagtcgacc gcacggaccg
    14581 ctcgcacccc gtcggcgagc gccgcgtcgg cgatgtcggc gcggaccgtc cggcgctggg
    14641 cggcgtacac gctgtccagg gtgcgctgga ggcggagtga gtagacggcg accaggtcgt
    14701 ccatcgcgtc gcggtgttcg agcgccgccg cagcgaaacg agtgtcggtg tcgaggacgt
    14761 ccgtgagacg accgaacaga ccgaggttgc ggcttcgtcc gtcgagcacg ctgtcgagct
    14821 cggcgtcgtc atcctcgcgg tcccgccacc cggccgtcac gatcgcaacg tgaccatcga
    14881 ccgacagtga gtgcaccgcc gccccggccg tggtcaggaa gcgttgtggg ccaaggagga
    14941 tcgtcctcat ccggcatgca cgcgcaccgt tcgccctgcc tcgtcgagca tgccccgcag
    15001 tgtgtcgtag tcgggatggc gcatccggat gtacgcgttg gccatgtacc cggcctcgac
    15061 cggctgggtc ggtgtcccgg ggttcggaag gtgcgcatcg atgatccagc gcccgtgacg
    15121 ctcgttgatc tcgtccaggc cgctgtatcc gcggatctgt ccgtcctgct ccggccggag
    15181 ggcgacgatg ccggcggagt actggcgcga cagcttgttc tccggcgacc cgtggacgat
    15241 ggcatgcgcc cactcgcggt agacgtcgac gtcgttggcg gccgagtaca ggtcccaggc
    15301 gcccacaccc ggcggccggc acccgatctc ggagaagcgc aggcccttcg gtccgtagaa
    15361 ccactccatg tgggtggccg acgtgggaat cccgagtgcc tcgatgaccc ggctgccgag
    15421 ctcgcgaacc tctgagtaga acaacgagtc ttcgatccgg ttggtcgtga cgaactgtgg
    15481 cgagatccag cggtgacgca tcgcttcgag cacgttgggg tagtagtgcg tcatccagtc
    15541 gtggacgatg tgcccgtcga tggtgatggt gtcgtagaac ccctcgtgac cctcgacgaa
    15601 ctcctccacc gcgatcgagt cggccgagcc gaagctcgcg agagccgctt ggagctcggc
    15661 catggagtcg accctggtcg tcccctgggc acctgcgcct gctctgggct tgaggatgag
    15721 cgggaaaccg atccggttcg cgaagtcgat gacctcgtcg gcgttgtggg ctgccgtcga
    15781 cgcggccgtc ggcacaccag cctgccgaag cgcttccttc atcgacggct tgtcccggca
    15841 cagccacgtc gtacgcacgg acgtccccgg aatcccgcgc gcctcacgga cctgagcggc
    15901 cgccatctgg tgggcctcga tggtcgactc cagccggtcg atccacgcaa ggttctgtgc
    15961 ccattccgta gccgcgttca gctgtccgat gtcggtgacg ttcgagacct tgtagtaggc
    16021 gcccatctgt ccgcgtagct cgtccccgag ctcgtgctcg gagtactcac cgaccccgat
    16081 cacgttggcg ccgacccccg ccagagcatg cacgaaccgg cgctggttgt gtggaaagaa
    16141 gggctccacg aagacgatgt tcacccgagc gactctagac gatcgccgta cgctcggctg
    16201 atggagcaca agcccgtcgt ctacttgatc acggggccga tggcagcggg aaaatcgacg
    16261 gttgcgcgcc tgctcgccgc acgcttcgag cgtggcgttc acctcgaagg ggacctgttc
    16321 cgtcgcagca tcgtcagcgg gcgtgaggag atgacgccgc acccgtcgcc ggcggcgctc
    16381 gagcagctcg gcctgcgcta tcgcttggcg gctgccgccg ccgacgcgta cgtcgaggcc
    16441 ggcttcagcg tcgcgctcga agatgtggcg gcaggaggct ggctgggcga ctaccgcacc
    16501 atgatccgca gccgcccctg tcacgtggtc gtcctgctcc cgtctgtcga ggttatcacc
    16561 gcgcgggaag ccggacggga ccacaagggg tacagcacct ggacgatcga gcagctctac
    16621 gaaggcttcg ccggctcgac gccgcgcgtc ggcatctggc tcgacacgac gcacctcact
    16681 ccggagcaga cggtcgacga gatcctctcc cagaccgatt cgacccgctc gccgattgtc
    16741 gtcagcgact acgacgatcg gtggcccgac gccttcgagc agatcgcgaa gccggtccgc
    16801 gctgcggtcg ccg
//
LOCUS       DATBFZ010000486         5038 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_6098300, whole genome shotgun sequence.
ACCESSION   DATBFZ010000486 DATBFZ010000000
VERSION     DATBFZ010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 5038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5038
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6098300"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="VH419_14810"
     CDS             complement(<1..266)
                     /locus_tag="VH419_14810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044937.1"
                     /translation="MSELLKDVMNERAGAAHIPDLDVASIIATGDRRIRRRRVAVGMA
                     AVSAVVAAAVAVPAVVDHDSAGRRTPPATKGSFETHTTTYALGT"
     gene            complement(266..799)
                     /locus_tag="VH419_14815"
     CDS             complement(266..799)
                     /locus_tag="VH419_14815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020384898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigE family RNA polymerase sigma factor"
                     /protein_id="HEY7044938.1"
                     /translation="MSSRDSAAFAEFVAARSTALFRAAYLMVGDHALAEDLLQEALTK
                     TYVAWPRLRDINKAEAYTRKAITTTAISWWRRKAWKHEKPRDDVPDVGHEGQTEQVAQ
                     RDWLWQELQNLAPKQRAAIVLRYYEDLTEAQTAEALGVSVGTVKSQVSDGLKKLRSIL
                     GADVVLVANGSDGMAVG"
     gene            complement(1276..3276)
                     /locus_tag="VH419_14820"
     CDS             complement(1276..3276)
                     /locus_tag="VH419_14820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020574071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY7044939.1"
                     /translation="MAPRFWGSRRRGSGGYRYGAWHGGPDPLAPPFDVRAAVDEIGDD
                     MLAGKSVRSALRDLLRRGTDGRRGLADLRRDVERRRRNLRRRGDLAGTLDRVQQMLDQ
                     VLAAEREQLAGEDSDEARLAEMTLDDLPAQIGSAVRELADYEWHSDEARSGYEQIRQM
                     LQREVLDAQFAGMKQALSGNDPEAMQRVRDMLADLNQLLSDHARGQDTPEQFEAFMER
                     HGEFFPDNPRDVEELIDSLARRQAAAERMLRSLSPGQRAELADLMAQALDDVDLQSQL
                     AQLQDNLAALRPGMGRGEPVDIRGNEPLGYGEAVGAVADLADLEALERQLAQDYPGAT
                     LDDVDVEALERQLAASDVADLETLRQLEAELERQGYIRRDPEGLSLTPKALRRLGESA
                     LRRIFAQLDADARGDHDDQRSGAADERTGAFLPWQFGDERPIDAVRTVQNAVLRRASQ
                     PRETDQPRLHVDDFTVAETERRTTAAVALCVDLSFSMVQEDRWQPMKQTALALAHLIG
                     TRYRSDALEIIGFDRTARRLTPLQLAEVEPEWVQGTNLQHALKIAGRHLRRHPDAEPV
                     VLVVTDGEPTAHLDTDGYPLFHWPPTAETVQATVAEVDAMTRLGATLNFFLLGDDPGL
                     ARFVDAVARRNGGRVLTPEPSRLGEYVVADYLRARRGHRRSA"
     gene            complement(3278..4681)
                     /locus_tag="VH419_14825"
     CDS             complement(3278..4681)
                     /locus_tag="VH419_14825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008359263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEY7044940.1"
                     /translation="MTANTPAPQIVTLHDLKSSGHEFRTVKEEVRRNLAERLRSGEPT
                     FPGIVGFDNTVLPELETALLAGHDLVLLGERGQGKTRVMRTLAGLLDEWTPVVAGCEI
                     NDHPYSPACLRCRSMVAEIGDDVPVAWRHRSLRYGEKLATPDTSVGDLVGDIDPTKVA
                     EGRLLGDPETVHYGLLPRANRGIFGLNELPDLAERIQVALFNVLEERDIQIRGYALRL
                     PLDLLLVASANPEDYTNRGRIITPLKDRFGAEIRTHYPLDIADEIELVTQESELVAEV
                     PQHLLEVVARFTDELRGSSAVDQRSGVSARFPVAAAEGIAGAALRRSALCGEDHAVAR
                     IGDLPGVLVTLQGKVEFEMGEEGREDEVFDHLLRLSTAATYREHLAGVDLNGFTQHFV
                     DGDTVETGALVPAADLLAQLGTIPGLSSVLDGLGVGEDNVSPGQVAAAVEFVLEGLHL
                     TRRLSKDSVDGTTIYGT"
     gene            complement(4781..>5038)
                     /locus_tag="VH419_14830"
     CDS             complement(4781..>5038)
                     /locus_tag="VH419_14830"
                     /inference="COORDINATES: protein motif:HMM:NF018647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HEY7044941.1"
                     /translation="PGGLVVFAVNRSRSEPIKLDLDLRAFDGLDRVEATTLTHDDPIA
                     RNVAADPDRVIPRPLADVRLDCDHLDAVLPALSWNVLRLTG"
BASE COUNT          692 a         1739 c         1775 g          832 t
ORIGIN      
        1 ctcgtgccga gcgcgtaggt cgtcgtgtgc gtctcgaagc tgcccttggt ggccggcggc
       61 gtacggcgcc cggctgagtc atggtcgacg acagccggga cggccaccgc ggcagcgacc
      121 acggccgaga ccgccgccat tcccaccgcg acccgccgac gccggatgcg tcggtcgccg
      181 gtggcgatga tcgacgccac gtcgaggtct gggatgtggg ccgcgcccgc ccgttcgttc
      241 atgacgtcct tcagcagttc actcatcacc ccaccgccat cccatcggac ccgttcgcca
      301 cgagcacgac atcggcgccc aggatcgacc tgagcttctt caagccatcg gagacctgac
      361 tcttgacggt gcccacgctg acaccgagtg cctcggcggt ctgcgcctcg gtcaggtcct
      421 cgtagtagcg cagcacgatc gctgcccgct gcttcggtgc gaggttctgc agctcctgcc
      481 acaaccagtc gcgctgggcg acctgctcgg tctgcccttc gtggccgacg tccggcacgt
      541 cgtcgcgcgg cttctcgtgc ttccaggcct tgcgccgcca ccacgagatc gcggtcgtcg
      601 tgatcgcctt gcgcgtgtac gcctccgcct tgttgatgtc acgcagccgt ggccaggcga
      661 cgtacgtctt ggtcaacgcc tcctggagca ggtcctcggc cagcgcgtga tcgccgacca
      721 tgaggtacgc cgcccggaac agcgctgtcg accgcgcagc cacgaactcc gcgaacgcgg
      781 cgctgtcgcg gcttgacatc gttgctcccg gggctggctg gtcggtcccc ttcgggcctc
      841 tctcaccctg acgtggtcag ccttgcaaat ggttgggttc ccggccatgc cttccggaca
      901 ggtcgctcaa cccgtgcggg tccgcgatgg agacatggca acccccgttt cgacggcagg
      961 tcgggagact gctcaggttt ctgtggccgg ggatggtcgc gggggcttgg cgcgatcgcc
     1021 cggcgttcgg tcgtcatggg ctgcgggtgg gttcgcagtg aaaagcgggc agcgtccgcg
     1081 cccgttgagc aggtcggccg gcgctccgtt gcgttcgcct ggtgcgtgac tcgcggtttt
     1141 cggacatccg cccttggccg ttcccggttg cctcgggcac catgcaaagc caccggtcgc
     1201 gccgcaagtc tgcctggcta cccgtgcatc cggatggcta cccaggcaaa ccaccaccgg
     1261 tgagcgcccc cgacgtcagg cggagcggcg gtggccgcgg cgggcgcgca ggtagtcggc
     1321 gaccacgtac tcaccgaggc ggctcggttc aggcgtcagg acgcgcccac cgttgcgccg
     1381 cgcaaccgcg tcgacgaagc gggcgagacc ggggtcgtct ccgagcagga agaagttcag
     1441 cgttgctccg agacgtgtca tcgcgtccac ctcggcgacc gtcgcctgca cggtttccgc
     1501 ggtcgggggc cagtggaaca aggggtaacc gtcagtgtcg aggtgtgcgg tgggctcgcc
     1561 gtcggtgacc acgagcacga ccggctcggc atcgggatgt cgacgcaggt gcctgcccgc
     1621 gatcttgagc gcgtgctgca ggttggtgcc ctggacccat tccggctcga cctcggcgag
     1681 ctggagcggg gtcagccggc gggcggtgcg gtcgaagccg atgatctcca gcgcatcgct
     1741 gcggtagcgg gtgccgatca ggtgggcgag cgcgagcgcg gtctgcttca tcggttgcca
     1801 gcggtcttcc tggaccatcg agaacgacag gtcgacgcac agcgccacag ccgcggtcgt
     1861 acgccgctcg gtctccgcca cggtgaagtc gtcgacgtgc aggcggggct ggtctgtctc
     1921 ccggggctgg gacgctctgc ggaggacggc gttctgcacc gtgcggaccg cgtcgatcgg
     1981 ccgctcgtcc ccgaactgcc agggcaggaa ggcgccggtc cgctcgtcgg cggctccgct
     2041 tcgttggtcg tcgtggtcgc ctcgcgcgtc ggcgtcgagc tgggcgaaga tccgtcgcag
     2101 cgcgctctcg ccgagccggc gtagtgcctt gggtgtcagc gacaggcctt ccgggtcgcg
     2161 tcggatgtac ccctggcgct ccagctccgc ctcgagctgg cgaagcgtct cgaggtccgc
     2221 cacgtcggac gcggcgagct gccgttcgag cgcttcgacg tcgacgtcat cgagcgtcgc
     2281 ccctgggtag tcctgtgcca gctgacgctc cagcgcctcg aggtcggcaa ggtcggcgac
     2341 ggcaccgacg gcctcgccgt acccgagcgg ctcgttcccg cggatgtcga ccggctctcc
     2401 tcgtcccatg ccgggccgaa gtgcggcgag gttgtcctgg agctgggcca gctggctctg
     2461 caggtcgacg tcatccagtg cctgcgccat gaggtcggcg agctcggcac gctgcccagg
     2521 gctcaacgag cgcagcatgc gttcggccgc ggcctggcgc cgggccagcg agtcgatgag
     2581 ctcctcgacg tctcgggggt tgtccgggaa gaactcgccg tgccgctcca tgaacgcctc
     2641 gaactgctcc ggcgtgtcct ggccacgtgc gtggtcggac agcagctggt tcaggtcggc
     2701 cagcatgtca cggacccgct gcatcgcctc ggggtcgttc ccggagagcg cctgcttcat
     2761 gccggcgaac tgcgcgtcga gcacctcacg ctggagcatc tgacggatct gctcgtaccc
     2821 cgaccgggcc tcgtcggaat gccactcgta gtcggccagc tcgcggaccg cgctcccgat
     2881 ctgtgccggc aggtcgtcca gcgtcatctc ggcgagcctg gcctcgtcgc tgtcctcccc
     2941 ggcgagctgc tcgcgttccg cggccaggac ctggtcgagc atctgttgga cacggtcgag
     3001 cgttcctgcg aggtcgccac gtcgacgcag gttgcgacga cggcgctcga catcacgccg
     3061 gaggtcggcg agtccgcgtc gaccgtcggt gccccgacga agcaggtccc gcagcgccga
     3121 acgcacggac ttgcccgcca gcatgtcgtc gccgatctcg tcgacggccg cccggacgtc
     3181 gaacggcggg gcgagcggat ccgggccgcc gtgccaggcg ccgtaccggt agccgccgga
     3241 cccacggcgg cgggagcccc agaaccgcgg tgccacgtca ggtcccgtag atcgtcgtcc
     3301 cgtcgacgct gtccttggac agccgacggg tgaggtgcaa cccctccagc acgaactcga
     3361 ccgcggcggc gacctggccg ggtgagacgt tgtcctcccc gacgccgagg ccgtcgagca
     3421 ccgacgacag gccggggatc gtgccgagct gtgccagcag gtcggcggcc ggcacgagcg
     3481 cccctgtctc gacggtgtcc ccgtcgacga agtgctgggt gaagccgttg aggtcgactc
     3541 ccgcgaggtg ctcgcggtac gtcgcagcgg tcgacaggcg tagcaggtgg tcgaagacct
     3601 cgtcctcgcg gccttcctcg cccatctcga actcgacctt gccctgcagt gtgaccagca
     3661 cgcccggcag gtcaccgatc ctggcgacgg catggtcctc gccgcacagc gcggaccgtc
     3721 gcagcgctgc cccggcgatg ccctccgctg ccgccacggg gaaccgtgcg gacacgccgg
     3781 agcgctggtc gactgcgctc gagccacgca gctcgtcggt gaacctcgcc accacctcga
     3841 ggaggtgctg gggcacttcg gcgacgagct cgctctcctg ggtgaccagc tcgatctcgt
     3901 cggcgatgtc gagcgggtag tgggtgcgga tctccgcacc gaaccggtcc ttgagcggtg
     3961 tgatgatgcg gccgcggttg gtgtagtcct cggggttcgc cgaggcgacg agcaacaggt
     4021 cgagcggcag ccggagcgcg tagccacgaa tctggatgtc ccgctcctcg agcacgttga
     4081 acagcgcgac ctggatgcgc tccgcgaggt cggggagctc gttgagcccg aagatgccgc
     4141 gattcgcccg cggcagcagt ccgtagtgga cggtttccgg atctcccagc agcctgcctt
     4201 cggccacctt ggtcgggtcg atgtcaccca ccaggtcgcc gacgctggta tccggcgtgg
     4261 cgagcttctc gccgtaccgg agcgatcggt gccgccaggc gaccggcacg tcgtcgccga
     4321 tctcggcgac catcgatcgg caccgtaggc acgcggggga gtaggggtgg tcgttgatct
     4381 cgcagccggc gaccaccggc gtccactcgt cgagcagccc ggcgagggtc cgcatcactc
     4441 gcgtcttgcc ctggccgcgt tcgccgagca ggaccaggtc gtgaccggcg agcagggcgg
     4501 tctcgagctc gggcaggacg gtgttgtcga acccgacgat gcccgggaac gtcggttcac
     4561 cgctacggag tcgttcggcc aggttgcgcc ggacctcctc cttcaccgtt cggaactcgt
     4621 gaccggagga cttgaggtcg tgaagggtga cgatctgcgg tgcgggcgtg ttggcagtca
     4681 cctgacccac ggtacgaccg ccggcaaacc cgcggaatgc gggaggttcc gcgatttcga
     4741 cggcagaaat cgcggaactc cccgcaattc gcggggaggc tcagccggtg aggcggagga
     4801 cgttccagga gagggcgggg aggacggcgt cgaggtgatc gcagtcaaga cgtacgtcgg
     4861 ccaacggcct gggtatcacc cggtccggat cggcggcaac gttgcgcgca atcgggtcgt
     4921 cgtgcgtcag cgtcgtggcc tcgacccgat ccagaccgtc gaacgcacgg aggtccaggt
     4981 ccagcttgat cggctcgctc cggctgcggt tgacggcgaa cacgacgaga ccacccgg
//
LOCUS       DATBFZ010000487         9685 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_5439531, whole genome shotgun sequence.
ACCESSION   DATBFZ010000487 DATBFZ010000000
VERSION     DATBFZ010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 9685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9685
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5439531"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..720
                     /locus_tag="VH419_14835"
     CDS             <1..720
                     /locus_tag="VH419_14835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020388090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY family transport protein"
                     /protein_id="HEY7044942.1"
                     /translation="IFTSVISAFPTALWGIKKEHGWDVFAIVAGIGLVIIAAVIFIEQ
                     AQRRIPVQYARRMVGRRMFGGSSTYIPLKVNQAGVIPVIFASSLLYLPALFSQFNQTS
                     GWSAWVQQHLVRGDHPYYMITYFLLIVFFTYFYVAITFNPQEVADNMKKYGGFVPGIR
                     AGKPTEDYLAYVLSRITFPGSIYLALVAIVPLIAFVVVNANQNFPFGGTSLLIMVGVG
                     LDTVKQIESQLQQRHYEGFLR"
     gene            720..1391
                     /locus_tag="VH419_14840"
     CDS             720..1391
                     /locus_tag="VH419_14840"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002517589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEY7044943.1"
                     /translation="MRVVLVGAPGAGKGTQAKFIAEHFAIPAISTGDIFRANIAARTE
                     LGQQAKKYMDAGDLVPDQVTIGIVRDRLSQDDAESGFLLDGFPRTLAQAEALRDLLDE
                     LGKPLDVVLELQVDDNEIIRRLSGRRTCRKCGHVWHVEFDPPKVEGVCDVCGGELFQR
                     DDDMPETIRRRLEVYTEQTAPLVGFYDDAGLLRSVSASGRVDQITEAAIAALTAATAD
                     ASAEV"
     gene            1405..2217
                     /gene="map"
                     /locus_tag="VH419_14845"
     CDS             1405..2217
                     /gene="map"
                     /locus_tag="VH419_14845"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007078614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEY7044944.1"
                     /translation="MFRERGVEIKSPDQIALMRRAGLVVAETLSLLGSAVRPGVTTKQ
                     LDAIAEDHIRTRGATPNFLGYHGFPATICASVNDEVVHGIPGDRVLRDGDVVSLDCGA
                     IVDGWHGDAAITVPVGDVRPELVELMRVCEEGLWRGFAAARLGGRVTDISHAIETFVH
                     SQGDYGIVEDYVGHGIGSAMHMPPSVPNYGRPGKGPKLVTGMALAVEPMITLGGIDTR
                     TLEDDWTVVTDDGSWSAHFEHSFTLTPDGALILTALDGGAAKLAELGVPCAA"
     gene            2535..5447
                     /locus_tag="VH419_14850"
     CDS             2535..5447
                     /locus_tag="VH419_14850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020574685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HEY7044945.1"
                     /translation="MEADPGYDVGALVRRLRTELRGTVADDPKAKALYAVDASNYRVV
                     PDVVVVPRDVDDLMTAVVTTAGVHAPVTLRGGGTSMAGNAIGGVVIDVSRHLTKILEL
                     DPGSRTALVEPGVVLTDLLAAAAPHGLTFGADPSSASRATLGGMIANNACGAHSVAWG
                     TTSDNLRRLEVLLPDGEQVALDAPEPGADPMKRRRELEARPGRAGVLARDLLALGDEH
                     ERLISRRFGTFSRQISGYALQHLLPDRGHDLARLLCGSEGSLATTLRAAIALTPVPAA
                     VALLAVGYPDAGTAADSVPVLLTHHPLTVESINATLVDRLPEPVRREAERAGLPPGQT
                     WLLVEIAGESADDAARLAEAVAADLADLEPRAASVVVVDRRAQAALWRCRRDAAGLAT
                     RRPDGAEAWGGWEDAAVPPARLGEYLRRLDELLARHRLTGASYGHFGEGCMHIRLDFD
                     LFTDAGVAAYRLFVEEACDLVVSLGGSASGEHGDGRARSALLARMYGADVLAVFADVK
                     KVFDPEGVMNPHIIVGAGEVDDGLRHLGRTSTRQLQTRFAYTADAGSFERAQRRCVGV
                     GKCRQSHGGVMCPSFQVTREEQHSTRGRAHLLWEMLQGDVVTGGWRSTEVKEALDLCL
                     SCKGCLADCPVNVDMATYKAEFTHAYYRGRQLARPRSHWSLGWLPVWARVAATAPRLT
                     NRLVTPPFDGLVKRAGGIAATRRLPAFSDQSFVLWFGGRPVQDLGGRRGRVLLWPDTF
                     TNFLAADVGRSAVAVLEDAGFEVVVPRRPVCCGLTWVSTGQLGLAARVLRRSLRRVAP
                     YVDAGVPVVALEPSCAAMLRHDGPGLLHRDPRAAAVAGATYTLAQLLERFAPDWRPPK
                     VERDALVQVHCHQHAVLGFDADAALLAAADVAVTRPDSGCCGLAGNFGLEPGHDEVSR
                     AAGERVLLPAVRAAAPETLIVADGFSCRTQIGQLTDRRAYHLAQVLAPSRGGRSRE"
     gene            5736..5957
                     /gene="infA"
                     /locus_tag="VH419_14855"
     CDS             5736..5957
                     /gene="infA"
                     /locus_tag="VH419_14855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEY7044946.1"
                     /translation="MPKKEGVIEIEGTVVEALPNAMFRVELNNGHKVLAHISGKMRQH
                     YIRILPEDRVVVELSPYDLTRGRIVYRYK"
     gene            6043..6156
                     /gene="rpmJ"
                     /locus_tag="VH419_14860"
     CDS             6043..6156
                     /gene="rpmJ"
                     /locus_tag="VH419_14860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEY7044947.1"
                     /translation="MKVNPSVKKICDKCKVIRRNGRVMVICANPRHKQRQG"
     gene            6375..6752
                     /gene="rpsM"
                     /locus_tag="VH419_14865"
     CDS             6375..6752
                     /gene="rpsM"
                     /locus_tag="VH419_14865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016637793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEY7044948.1"
                     /translation="MARLVGVDLPRDKRIEVALTYIFGIGKTRALETLDATGVSPDMR
                     VHQLGDEELVKLRDHIEANYQIEGDLRREVTADIRRKVEIGSYQGRRHRQGLPVRGQR
                     TKTNARTRKGPKRTVAGKKKAGK"
     gene            6847..7254
                     /gene="rpsK"
                     /locus_tag="VH419_14870"
     CDS             6847..7254
                     /gene="rpsK"
                     /locus_tag="VH419_14870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008747488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEY7044949.1"
                     /translation="MPPKGRQAAGAKKVRRKEKKNVGAGHAHIKSTFNNTIITITDPY
                     GAVISWASAGTVGFKGSRKSTPFAAQMAAEAAGRRAMEHGMKKIDVFVKGPGSGRETA
                     IRSLGAVGLEVGTIQDVTPSPHNGCRPPKRPRV"
     gene            7285..7893
                     /gene="rpsD"
                     /locus_tag="VH419_14875"
     CDS             7285..7893
                     /gene="rpsD"
                     /locus_tag="VH419_14875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEY7044950.1"
                     /translation="MARYTGPMTKKSRRLGVDLIGGDAAYERRPYPPGQHGRGRVKES
                     EYRNQLMEKQKARYTYGVLEKQFRNYYKEASRRPGKTGDNLLQMLESRLDNVVYRAGF
                     ARTRRHARQLVVHGHFLVNGRKVDIPSYQVAAHDVIDVRPKSVETTPFIIARETHGER
                     IVPAWLEVIPSRMRVLVHQLPVRQQIDVPVQEQLIVEYYSKK"
     gene            8055..9071
                     /locus_tag="VH419_14880"
     CDS             8055..9071
                     /locus_tag="VH419_14880"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018538739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEY7044951.1"
                     /translation="MLIAQRPSLSEDVVDDHRSRFSIEPLEPGFGYTLGNSLRRTLLS
                     SIPGAAVTSIRIDGVLHEFSTISGVREDVTEVILNLKQLVVSSEHDEPVTMYLRKQGP
                     GEVTAADIAPPAGVEVHNPELVLANLNDKGKLDMELVVERGRGYVSAVQNKAGDTEIG
                     RMPVDSIYSPVLKVTYKVEATRVEQRTDFDRLIIDVETKPSMKPRDALASAGKTLVEL
                     FGLARELNVEAEGIDIGPSPVDEQLAADLALPVEDLALTVRSYNCLKREGIHTVGELL
                     SRSEQDLLDIRNFGSKSIDEVKAKLAEMGLSLKDSAPGFDPSAVIDSYDEDDDTSFAE
                     DEQY"
     gene            9242..>9685
                     /gene="rplQ"
                     /locus_tag="VH419_14885"
     CDS             9242..>9685
                     /gene="rplQ"
                     /locus_tag="VH419_14885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008361063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEY7044952.1"
                     /translation="MPTPKKGPRLGGSPAHQKAILANLATSLFEHGSITTTEAKARVL
                     RPYAEKLITKAKRGDLHNRRQVMSVIRDKSVVHVLFTEIGPRYSERPGGYTRITKIGP
                     RKGDNAPMAVIELVEEEFTSQARTTKKTASAKQAAAAPETEVEETT"
BASE COUNT         1622 a         3266 c         3263 g         1534 t
ORIGIN      
        1 atcttcacgt cggtgatctc ggcgttcccg accgcgttgt ggggcatcaa gaaggagcac
       61 ggctgggacg tgttcgcgat cgtcgcgggc atcggactgg tgatcatcgc cgcggtcatc
      121 ttcatcgagc aggcgcagcg ccgcatcccg gtccagtacg cccgtcggat ggtcggtcga
      181 cggatgttcg gcggcagctc gacgtacatc ccgctgaagg tcaaccaggc cggcgtcatc
      241 ccggtcatct tcgcgagcag cctgctctac cttcccgcac tgttctcgca gttcaaccag
      301 acctccgggt ggtcggcgtg ggtgcagcag cacctcgtcc gtggtgacca cccgtactac
      361 atgatcacgt acttcctgct gatcgtgttc ttcacgtact tctacgtggc gatcacgttc
      421 aatccgcagg aggtcgcaga caacatgaag aagtacggcg gtttcgtacc tgggatccgg
      481 gccggtaagc cgaccgagga ctacctcgcc tacgtgctgt ctcggatcac gttcccgggc
      541 tcgatctacc tcgccctggt cgcgatcgtg ccgctcatcg ctttcgtcgt cgtcaacgcg
      601 aaccagaact tcccgttcgg cgggacctca ctcctcatca tggtgggcgt cggactcgac
      661 acggtgaaac agatcgagag ccagctgcag cagcgacact acgaaggatt ccttcgctga
      721 tgcgcgttgt cctggtcggt gcgcctggag cgggcaaggg gacacaggca aagttcatcg
      781 ccgagcactt cgccatccca gcgatctcga cgggcgacat cttccgggcg aacatcgcgg
      841 ccaggaccga actcggccag caggccaaga agtacatgga tgccggcgac ctcgtgccgg
      901 accaggtgac gatcggcatc gtgcgcgacc ggctgagcca ggacgacgcg gagtcgggct
      961 tcctgctcga cggcttcccg cgcacactgg cacaagccga agcgcttcgc gacctgctcg
     1021 acgagctggg caagccgctc gacgtcgtcc tcgagctgca ggtcgacgac aacgagatca
     1081 tccgccggct gtcaggacgt cgtacctgcc gcaagtgcgg ccacgtctgg cacgtcgagt
     1141 tcgacccgcc gaaggtcgag ggagtctgcg acgtctgtgg cggcgagctc ttccagcgtg
     1201 acgacgacat gccggagacg atccgccggc ggctcgaggt gtacaccgag cagaccgctc
     1261 cgctcgtggg gttctacgac gacgccggcc tgttgcgctc cgtctccgct tcgggccggg
     1321 tcgaccagat caccgaggcc gccatcgcag ccctgaccgc cgccaccgcc gacgcgtcgg
     1381 cagaggtctg acgggcgccg gcgcatgttc cgcgagcgag gcgtcgagat caagtcaccc
     1441 gaccagatcg cgctgatgcg aagagctggt ctcgtggtgg ccgagacact gtctctgctg
     1501 gggtcggctg tgcggcccgg ggtgaccacg aagcagctgg acgcgatcgc cgaagaccac
     1561 atccggactc gcggcgccac gcccaacttc ctcggctacc acggctttcc tgcaacgatc
     1621 tgcgcgtccg tcaacgacga ggtcgtccac gggatccctg gcgatcgtgt cctgcgtgac
     1681 ggcgatgtcg tgtcgctgga ctgcggcgcg atcgtcgacg gctggcacgg tgacgcggcc
     1741 atcacggtgc ccgtcggcga cgtccgtcct gagctggtcg agctcatgcg ggtttgcgag
     1801 gagggtcttt ggcgcggttt cgcggctgcc cgactgggcg ggcgggtcac cgacatctcg
     1861 cacgcgatcg agacctttgt gcacagccag ggtgactacg gcatcgtcga ggactacgtg
     1921 ggtcatggca tcgggtcggc gatgcacatg ccgccgagcg tgccgaacta cggtcgtccc
     1981 ggcaaaggac cgaagctggt caccggcatg gcgctggccg tcgagccgat gatcaccctc
     2041 ggtggcatcg acaccaggac cctcgaggac gactggaccg tcgtgaccga tgacggcagc
     2101 tggtccgcgc acttcgagca ctcgttcacg ttgactccgg acggcgcact gatcctgacc
     2161 gccctggacg gcggtgccgc gaaactcgcc gagctcggcg tcccctgcgc cgcctgaccc
     2221 tcccgccgag gtggcagctt tcgttcactt ctgacggcgt gtcgtgcgac gagtgccacc
     2281 tcgatcggtt ctccgcggct gatcaggcgc accgttggca gcggctgagc tgatcgccgg
     2341 cgaagagcgg gagacgtgtt tgctcgctga gccggtcggc cgagcttccg ctccttgtgg
     2401 tcgctgggtg acggtttgca tggctaccca cgcaagctca cccgtaccaa ggcgggtttg
     2461 cctggctacc cgtgcgcttg gatggctacc caggcaaacc acgagggacg gtcggggtgc
     2521 gtcagactgc tgggatggag gcggacccgg ggtatgacgt cggggcgctg gtccgccggc
     2581 tccgaactga gctgcgggga accgtcgcgg atgatcccaa agcgaaggcg ttgtacgccg
     2641 tcgacgcgtc gaactatcgc gtcgtgcccg acgtcgtcgt cgtaccgcgg gatgtcgacg
     2701 acctgatgac ggccgtcgtg accaccgccg gagtccacgc gcccgtcacg ttgcggggcg
     2761 gcggtacgtc gatggcgggc aacgcgatcg gtggtgtcgt gatcgacgtg tcgcgacatc
     2821 tcacgaagat cctcgagctc gatcccggta gccgcacggc gctcgtcgag cccggagtcg
     2881 tactcaccga cctgctggca gcggccgctc ctcacgggct gacgttcggc gctgatccgt
     2941 cgtcggccag ccgggccacc ctcggcggga tgatcgccaa caacgcctgc ggcgcacact
     3001 ccgtcgcctg ggggacgacg tcggacaacc tgcgcaggct cgaggtgttg ttgcccgacg
     3061 gcgagcaggt ggcgctcgac gcgccggagc cgggcgccga tccgatgaaa cgccgccgtg
     3121 agctggaggc gcggccgggt cgcgcaggcg tactcgcccg cgacctcctc gcgctcggcg
     3181 acgagcatga gcgactcatc agccgccggt tcgggacgtt ctcgcggcag atctccggct
     3241 acgccttgca gcacctgctg cccgaccgcg ggcacgacct ggctcggctg ctgtgtggca
     3301 gtgaggggag cctcgcgacg acactgcgcg cagcgatcgc cctcacgccg gtacccgcgg
     3361 ccgtcgcgct cctggcggtc ggttacccgg atgcgggtac cgccgccgac agcgtcccgg
     3421 tccttctcac ccaccacccg ctcaccgtgg agtcgatcaa cgccacgctt gtcgaccggc
     3481 tgccggagcc ggtacgccga gaggcggagc gggcgggcct accgccgggg cagacctggc
     3541 tgctcgtcga gatcgcaggc gaatcagcag acgacgccgc ccgactggcg gaggcggtcg
     3601 cggcagacct cgccgatctg gagccacgag ccgcttcggt ggtcgtggtc gatcgacgcg
     3661 cgcaggccgc cctgtggcga tgccgccgcg acgccgccgg gttggcgact cggcggcccg
     3721 acggggccga agcctggggt ggttgggagg acgcggccgt gccgcccgct cggctgggcg
     3781 agtacctccg tcggctcgac gagctgctcg cacgccatcg actgacggga gcgtcgtacg
     3841 ggcatttcgg tgagggctgc atgcacatcc ggctggactt cgacctcttc acagacgcgg
     3901 gcgttgcggc gtaccgattg ttcgtcgagg aggcctgcga cctcgtcgtc tcactcggcg
     3961 gctcggcctc aggcgagcat ggcgatggcc gcgcccggtc ggcactgctg gcgcggatgt
     4021 acggcgcaga cgtgcttgcc gtcttcgccg acgtcaagaa ggtcttcgac ccggaaggcg
     4081 tgatgaaccc gcacatcatc gtgggtgcgg gcgaggtcga cgacggtctc cggcatctcg
     4141 ggcgcacgag cacacggcag ctgcagactc ggttcgcgta tacggccgac gccgggagct
     4201 tcgagcgggc gcaacggcga tgtgtgggtg tcgggaagtg ccgtcagtcg cacggtggcg
     4261 tgatgtgccc gagcttccag gtcacccgcg aggagcagca ctcgactcgc gggcgggcac
     4321 atctgctgtg ggagatgctc cagggtgacg tggtgacagg tggctggcgc tctaccgagg
     4381 tcaaggaggc gctggacctg tgtctgtcgt gcaaggggtg tcttgccgac tgtccggtca
     4441 acgtggacat ggccacctac aaggcggagt tcacccacgc gtactaccgc ggcaggcagc
     4501 ttgcgcggcc acgctcgcac tggtcgttgg gctggctgcc ggtgtgggcg cgggtcgcgg
     4561 caacggcccc tcggctcacg aaccgcctgg tcaccccgcc cttcgacggt ctcgtgaagc
     4621 gggccggtgg catcgcggcc acgcgccggc taccggcgtt ctccgaccag tccttcgtcc
     4681 tctggttcgg cggcagaccg gtgcaggacc tcggtggtcg tcgcggccgc gtcttgctgt
     4741 ggcccgacac gttcacgaac ttcctcgccg ccgacgtcgg gcgcagcgct gtcgccgtcc
     4801 tcgaggacgc cggcttcgaa gtggtggtgc cgcggcgacc cgtctgctgc ggcttgacgt
     4861 gggtgtcgac gggccagctc ggtttggcgg cacgcgtgct gcgacggtct ctgcgtcgcg
     4921 tcgcaccgta cgtcgacgcc ggggtccccg tcgttgccct ggagccgagc tgcgcggcga
     4981 tgctgcgcca cgacgggcct gggctgctcc accgggatcc acgtgccgcc gctgtggcgg
     5041 gagcgacgta cacgctcgcg cagctgctgg agcggttcgc ccctgactgg cgtccaccga
     5101 aggtcgaacg ggacgcgctg gtccaggtgc actgtcacca gcacgcggtg ctcggcttcg
     5161 atgcggacgc tgccctgctc gcggcggccg acgtggcggt cacccgtccc gactccggct
     5221 gttgcggcct cgccggcaac tttggcctcg aacccggcca tgacgaggtg tcgcgggctg
     5281 ccggtgagcg ggtcttgctg ccggcggttc gagcggcggc gccggagacg ctgatcgtcg
     5341 ccgacggctt cagctgccgg acccagatcg ggcagctgac cgaccggcgg gcgtatcacc
     5401 tggcacaggt cctcgctccc agccgaggtg gcaggtcccg cgaatagcgg gaaacgtcag
     5461 gttcggatcc gcgtgtcgac cgccactttc ccgcaattca cgggagcccg gcagcaggcg
     5521 gtcgggcagg tgcggctcgt cgattttggg tctgtgccgc ctctcccgta gacttggggg
     5581 tcggcccaga cgtgggcccg ttcctgtgtg ccctgcggct gcccgttggg gagggcgccc
     5641 ggagcggccg gatgcaccgt catccgctcg tcggaccgag tgaaccacct gccagacaac
     5701 cgccagacac aacactgctg aagattgcgg aggacatgcc gaagaaagaa ggggtcatcg
     5761 agatcgaggg caccgtcgtc gaggctttgc cgaacgccat gttccgggtc gagctcaaca
     5821 acgggcacaa ggttctcgcc cacatcagcg gcaagatgcg ccagcactac atccggatcc
     5881 ttcccgagga ccgtgtcgtc gtggagctgt cgccgtacga cctcaccagg ggtcgcatcg
     5941 tctaccggta caagtagcag tcccgtccct accagccgtc catccagcga tctgccgagg
     6001 ctcgagtgtg agcgtccggc accgcgcctc acggagagcc cgatgaaggt caacccgagc
     6061 gtcaagaaga tctgcgacaa gtgcaaagtg atccgccgca acggccgggt catggtgatc
     6121 tgcgccaacc cgcggcacaa gcagcgccag ggctgacccc ggctcaccgg caactcacac
     6181 aactcaacag caccaccaac aagcgcgacc gctcgtcgtc gtacgccgtc tggactccaa
     6241 cggagtacgc cgatgtcgcc cccggaacga aggccggggc cctgccatcc gatggtcgaa
     6301 ccgtcgacag atcgcagagg cgggcaggga cgcggcgcgc agacaccttc gacgactgaa
     6361 aggtataccg ccacatggca cgcctcgtcg gtgtcgacct cccgcgcgac aagcgcatcg
     6421 aggtcgcact cacctacatt ttcgggatcg gcaagactcg tgcactcgag acgctggacg
     6481 cgaccggggt cagccccgac atgcgcgtcc accagctcgg agacgaggag ctggtcaagc
     6541 ttcgtgacca catcgaagcg aactaccaga tcgagggtga ccttcgtcgt gaggtgaccg
     6601 ccgacatccg ccgcaaggtc gagatcggga gctaccaggg ccgccggcac cgccagggcc
     6661 tgcccgtccg tggtcagcgc accaagacca acgctcggac tcgcaagggt cccaagcgca
     6721 ccgttgccgg caagaagaag gcgggcaagt gacagcaggc cctgcgccac tgcagagcca
     6781 tgtcgctgcc gtctcccccc gactgctcag ctgaccagct acctcgaact caggagtttg
     6841 tgacctatgc ctcccaaggg ccgtcaggcc gccggcgcca agaaggtgcg ccgcaaggag
     6901 aagaagaacg tcggtgcggg ccacgcccac atcaagagca cgttcaacaa cacgatcatc
     6961 acgatcaccg acccgtacgg cgcggtgatc tcatgggcct ctgccggcac cgtcggtttc
     7021 aagggctccc gcaagtccac tcccttcgct gctcagatgg ccgcagaggc cgctgggcgt
     7081 cgggcgatgg agcacggcat gaagaagatc gacgtcttcg tcaagggccc cgggtcgggt
     7141 cgcgagaccg ccatccgttc gctgggtgcc gtcggcctcg aggtcggcac gatccaggac
     7201 gtcacgccga gcccgcacaa cggctgccgc ccgccgaagc ggccgcgcgt ctgagcgccc
     7261 gagcgagaga ggagacagtc tgacatggcc cgctacaccg gacccatgac gaagaagtcg
     7321 cgccgcctgg gggtcgacct catcggcggc gacgcggcgt acgaacgtcg tccctacccg
     7381 cccggccagc acggccgcgg ccgggtcaag gagtcggagt accgcaacca gttgatggag
     7441 aagcagaagg ctcgctacac gtacggcgtg ctggagaagc agttccgcaa ctactacaag
     7501 gaagccagcc gtcgtcccgg caagaccggt gacaacctgc tgcagatgct cgagtcgcgg
     7561 ctcgacaacg tcgtctaccg cgccggcttc gcccgtaccc gccgccacgc tcgccagctg
     7621 gtggtgcacg gccacttcct ggtcaacggc cggaaggtcg acatcccgtc gtaccaggtg
     7681 gccgcgcacg acgtgatcga cgtacgcccc aagtccgtcg agacgacccc gttcatcatc
     7741 gcccgtgaga cccacggcga gcggatcgtc ccggcctggc tcgaggtcat cccgagccgg
     7801 atgcgggtgc tcgtgcacca gctcccggtg cgtcagcaga tcgacgtgcc ggtccaggag
     7861 cagctcatcg tggagtacta ctcgaagaag tagctggtct tcgcggcttc gccgcgaagg
     7921 ctgcctgacg ggggcttcgc cccctacccc cgtctcatgg ctggggtcca gttggaccgt
     7981 ggttatgaaa cgggaggtcc gaaccgaata gctgcgtcat caaatagcgg gtggcgtagg
     8041 aaggaagaac tactgtgctc atcgcccagc gcccgtccct gtccgaagat gtcgtcgacg
     8101 accaccgctc ccggttctcc atcgagccgc tggagccggg tttcggctac accctcggca
     8161 actcgcttcg tcgtacgctg ctctcctcga ttccgggggc ggcggtcacg tccatccgaa
     8221 tcgacggtgt cctgcatgag ttctcgacga tctcgggggt ccgcgaggac gtcaccgagg
     8281 tcatcttgaa cctcaagcag ctcgtcgtct cctccgagca cgacgagccc gtcaccatgt
     8341 acctgcgcaa gcagggtcct ggtgaggtca ccgccgccga catcgcgccg ccggcgggtg
     8401 tcgaggtcca caaccccgag ctcgtcctgg ccaacctcaa cgacaagggc aagctcgaca
     8461 tggagctcgt cgtcgagcgt ggccgtggct acgtctcggc cgtccagaac aaggccggcg
     8521 acaccgagat cggccgcatg ccggtcgact cgatctactc gcccgtcctc aaggtcacgt
     8581 acaaggtcga ggcgacccgt gtcgagcagc gcaccgactt cgaccggctc atcatcgacg
     8641 tcgagaccaa gccgtcgatg aagccgcgcg acgcactcgc ctctgccggc aagaccctcg
     8701 tggagctgtt cggtctggcc cgggagctca acgtcgaggc cgaaggcatc gacatcggcc
     8761 cctcgccggt ggacgagcag cttgctgccg acctggcgct gcccgtcgag gacctcgcgc
     8821 tgacggtccg ctcgtacaac tgcctcaagc gtgagggcat ccacaccgtg ggtgagctgc
     8881 tctcccgcag tgagcaggac ctgttggaca tccgcaactt cggctcgaag tcgatcgacg
     8941 aggtcaaggc gaagctcgcc gagatggggt tgtcgttgaa ggactcggct cccggattcg
     9001 acccgagcgc cgtcatcgac tcgtacgacg aggacgacga cacgtcgttt gccgaggacg
     9061 agcagtactg acagagcacc ggtcgggccg gagagcgcag agcgcgaccc gacgggccgt
     9121 caaggagcgg agcgacaggc ccgagggcgt ggtcggagcg accgggccga agcgcacaac
     9181 tgaagagaag agcaagcatc caggtacctg acacggcctg gaggagaaga gagtagacat
     9241 catgcctacc ccgaagaagg ggccccgcct cggcggcagc ccggcacacc agaaggcgat
     9301 cctggcgaac ctggccacca gcctgttcga gcacggctcg atcacgacga ccgaggcgaa
     9361 ggcgcgtgtg ctgcgtccgt atgccgagaa gctcatcacg aaggcgaagc ggggcgacct
     9421 gcacaaccgc cgtcaggtga tgtcggtgat ccgcgacaag agcgtcgtac acgtgttgtt
     9481 caccgagatc gggccgcgat acagcgagcg tccgggcggc tacacccgca tcacgaagat
     9541 cggtccgcgc aagggcgaca acgccccgat ggcggtgatc gagctcgtcg aggaggagtt
     9601 cacctcgcag gcgcggacga cgaagaagac ggcatccgcg aagcaggcag cagcagctcc
     9661 ggagaccgag gtcgaggaaa ccacg
//
LOCUS       DATBFZ010000488         2993 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_4784250, whole genome shotgun sequence.
ACCESSION   DATBFZ010000488 DATBFZ010000000
VERSION     DATBFZ010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2993
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4784250"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /locus_tag="VH419_14890"
     CDS             complement(<1..594)
                     /locus_tag="VH419_14890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016666397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY7044953.1"
                     /translation="MSAPAARAVEVRKVYGAGDAAVVALDHINVDLQAGHFTAIMGPS
                     GSGKSTLLHVMAGLDRPDSGHVYLGDTEITSLKDRALTILRRDQLGFIFQSFNLLPTM
                     TAAENIVLPMRIAGRKADRAWIDKIVDIVGLEDRLTHRPAELSGGQQQRVAAARALAS
                     RPQIVFADEPTGALDSKSGAELLAFLQMAVKELGQTVV"
     gene            908..2230
                     /locus_tag="VH419_14895"
     CDS             908..2230
                     /locus_tag="VH419_14895"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEY7044954.1"
                     /translation="MSETTGWRRWRSSRRAAVDLVVAAGLAGFVVGTYVVVVLGGGAL
                     LGNTSSPEVGLSILATVMVALGFERVRRRLHAWVENVIHHGQPSPYDVLRQFSTTVTG
                     SSTEEEIAPRMAKALAEGTGSDWAQVWFLVHGRPQLAATWPPRADTGDFSPYSGRVDV
                     QTRQVRQAGELLALLAVRTADDASLGPVEQRLFAGLANQAGLVLRGARLRAELVVRAA
                     ELSIQQAELEASRERVVDAHDQGRRRLERDIHDGAQQHLVALSVNLRLAQTLADSSPV
                     RAERVLDEQTEAVATAIATLVDLSRGIYPRQLGEHGLATALRVAFESNPVPVVVTTSS
                     LARLASGTESALYFCALEAVQNATKHAAASMIRVDVGSTDDVVTVSIEDDGQGYAVER
                     PGNGAGLANMRDRIESVGGTLTVHSSPTRGTQVAASVPAMRLPEQRPG"
     gene            2232..>2993
                     /locus_tag="VH419_14900"
     CDS             2232..>2993
                     /locus_tag="VH419_14900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044955.1"
                     /translation="MLPRFAWFLTLVSGVCAAADTWITVASRPLNSERTLAVHGWPLV
                     TLATVGASAMGALIISRYPRHPIGWLFCVVGVTSAMSMVTEAFAMWHAADGRPGSMAL
                     ANVAAWISTVFGGASSLTGLTLIFLLVPDGHLLSRRWRYVAWTSVAGFATFLASLVLI
                     DPTSYTIIEPENASATAASLSTIGFLLITVAMVASVTSLVIRFRRSRGRTRQQLRLIV
                     VAGSLVAVGLATLLVVQLFNGGKQTWAAGLPLFVSY"
BASE COUNT          422 a         1006 c         1039 g          526 t
ORIGIN      
        1 gacgacggtc tggccgagct ccttgaccgc catctgcagg aaggcgagca gctcggcgcc
       61 agacttcgag tccagtgcgc cggtcggctc gtccgcgaag acgatctggg gccgtgaggc
      121 gagggcccgg gcagcagcga cgcgttgctg ttgaccgccc gacaactccg ccggtcggtg
      181 tgtcagccga tcttcgaggc cgacgatgtc gacgatcttg tcgatccacg cgcggtcggc
      241 cttgcgaccg gcgatgcgca tcggtagcac gatgttctcc gctgccgtca tcgtcggcag
      301 caggttgaag gactggaaga tgaagccgag ctggtcacgg cgcaggatgg tcagcgcacg
      361 gtccttcagc gaggtgatct cggtgtcccc gaggtacaca tgtccgctgt cgggccggtc
      421 gagaccggcc atcacgtgca gcagggtcga cttccccgac ccggacgggc ccatgatggc
      481 ggtgaagtga ccggcctgga ggtcgacgtt gatgtggtcg agcgcgacga cggcggcgtc
      541 gccggcgcca tacaccttcc gcacctcgac ggctctggcc gcgggtgccg agatcgagac
      601 tgcgccgttc ggtccggtcg gctggacggg gatggtcggg gtgcctcgcg cgtcggcgtg
      661 ttcggtggtg ctcgcggtcg actgggcatt gctggtcatg ggtgaggctc ctggatcggg
      721 ttggtccact cggtcgagag gaccaccacc agagaaccgt cgccgactcg agcccgacat
      781 ggtggtgagc gcacggctca gtgccgtccc ccgacccaga tttgccggtg aaccatgcgg
      841 acaaccgcac gtggcggttc ggtcgcttgc cctgtgagcg ctggcgcctt cggcgcacac
      901 tgtggagatg agcgaaacga ccgggtggcg gcgttggcgt agctcgcgcc gggcagctgt
      961 cgatctggtc gtcgcggccg gtctggccgg cttcgtcgtc ggcacctacg tggtcgtcgt
     1021 gctcggaggc ggtgcgctgt tgggcaacac gtcctcaccg gaggtcggtc tctcgatcct
     1081 cgccaccgtc atggtggcgc tcgggttcga gcgtgtccgt cggcggctgc atgcctgggt
     1141 ggagaacgtg atccaccacg gtcagccatc gccgtacgac gtcctccgcc agttctcgac
     1201 cacagtcacc ggatcgtcga ccgaggagga gatcgcgccg cggatggcca aggcactggc
     1261 cgaggggaca ggctcggact gggcacaggt gtggttcctg gtgcacggcc ggccccagct
     1321 cgccgcaacc tggccgccac gcgccgacac cggtgacttc agcccgtaca gcggccgcgt
     1381 cgatgtccag acgcgccagg ttcggcaggc tggtgagctg ctcgcactcc ttgccgtccg
     1441 caccgccgac gacgcgtcgc tgggcccggt cgaacagcgg ctgttcgccg gcctcgccaa
     1501 ccaggccggc ctggtcctgc gcggcgcgcg gctccgcgcc gagctggtcg tgcgggccgc
     1561 tgagctgtcg atccagcagg ccgagctgga ggcgtcccgc gagcgtgtcg tcgatgccca
     1621 cgaccaaggg cgccgccgcc tggaacgcga catccacgac ggtgcccagc aacatctcgt
     1681 ggctctgtcg gtgaaccttc ggctggcaca gacgttggca gacagctcgc cggtccgtgc
     1741 cgaacgggtg ctcgacgagc agaccgaagc cgtcgcgaca gccatcgcca ccctcgtcga
     1801 cctgtcgcgc ggaatctatc cgcggcaact gggtgagcac ggcctggcta ctgcactgcg
     1861 cgtcgcgttc gagtccaacc ccgtgcccgt cgtcgtaacc acgtcgtcac ttgcccgact
     1921 cgcttccggg acggagtccg cactgtactt ctgcgccctg gaggcagtgc agaacgccac
     1981 caagcacgca gcggcgagca tgatccgagt ggacgtcggc tcgaccgacg acgtcgtcac
     2041 cgtctcgatc gaggacgacg ggcagggcta tgccgtcgaa cggccgggca acggtgcagg
     2101 tcttgccaac atgcgcgacc gcatcgagtc ggtcggcggg accctgaccg tccactcctc
     2161 gccgacccgg gggacgcagg tcgccgcgag cgttcccgcg atgcggctac ccgagcagcg
     2221 gccgggctga catgctgccc cggttcgcct ggttcctcac cctcgtcagt ggtgtctgcg
     2281 cggctgctga cacctggatc accgttgcct cgaggccgct caacagcgag cggaccctgg
     2341 ctgtccacgg ctggccgttg gtgacgctgg ccacggtcgg ggcgtcggcc atgggagcgc
     2401 tgatcatctc tcgctatcca cggcacccga tcggctggct gttctgcgtc gtcggcgtga
     2461 ccagcgcgat gtcaatggtc accgaggcct tcgcgatgtg gcatgcggcc gacggacggc
     2521 ccggttcgat ggcgctggcg aacgtggcgg catggatttc gacggtgttc ggcggcgcgt
     2581 cctcgttgac cgggttgacg ctgatctttc tcctggtacc ggacggtcac ctgctgtcga
     2641 gacgctggcg gtacgtcgcc tggacatccg ttgccggctt cgccaccttc ctcgcgagcc
     2701 tggtgctgat cgacccgacg tcatacacca tcatcgagcc cgagaacgct tccgcaaccg
     2761 ccgcttcgct gtcgacgatc ggcttcctcc tcatcacggt ggcgatggtg gcgtcggtga
     2821 catcactcgt gatccggttc cggcgtagtc ggggcagaac gaggcagcag cttcgactca
     2881 tcgtggttgc cggctcattg gtcgcggtcg ggctcgccac cttgttggtc gtgcagctgt
     2941 tcaacggcgg caagcagacc tgggcggccg gcctcccgct gttcgtctcg tac
//
LOCUS       DATBFZ010000489         8963 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_6413822, whole genome shotgun sequence.
ACCESSION   DATBFZ010000489 DATBFZ010000000
VERSION     DATBFZ010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 8963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8963
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6413822"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="VH419_14905"
     CDS             complement(<1..235)
                     /locus_tag="VH419_14905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044956.1"
                     /translation="MTTVICRGSAVSVAEVGAVGAVGSVDWVVADVLGEEGVASTGGE
                     PVDGEFGLASESPWMVQPAASSTPAATSSARRYS"
     gene            234..1256
                     /locus_tag="VH419_14910"
     CDS             234..1256
                     /locus_tag="VH419_14910"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEY7044957.1"
                     /translation="MNGDMLLHEGLWESAQIDAARTGRGPDGMDFRPILADMRGVVSG
                     ADLAVCHLETPLAPHGGPYSGYPVFSVPPAILPALKWEGYDACTTASNHSLDQGFDGL
                     KRTIDDFDRIGLAHAGTAETQKASRQPLLLEVKGVKVALISATYGTNGIPLPADEPWS
                     VPLINPDRIERLAHRARSEGAEIVLVALHWGLEYMHAPTSDQIAIADELTQSPDIDFV
                     YGHHAHVVQPYDRIHGTWVVYGLGNAVAQQDTAVEGVYDGNTCRVTFTEQPDGSFKVS
                     RLEYIPTMITPFDGVHPMRWLNVPKDIHDPAYAGLRPELEATQGRVRTVVGSLGAFKH
                     GVTEGH"
     gene            1415..1816
                     /locus_tag="VH419_14915"
     CDS             1415..1816
                     /locus_tag="VH419_14915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044958.1"
                     /translation="MVPWIVTIAAALVFVGLLDCVLPKRRTHWTVRLGNNLRGLVSRL
                     RRSRVTDPDPFVVLRIQSRLGVLASEIRAVEADRFAYAKVRRLEALQAAYDDLLNEGC
                     KLAGVDAFETQDREAKRWREEQELAERGWSW"
     gene            1987..3753
                     /locus_tag="VH419_14920"
     CDS             1987..3753
                     /locus_tag="VH419_14920"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012920649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEY7044959.1"
                     /translation="MSMRMSSLFLRTLREDPADAEIPSHKLLVRAGYIRRAAPGIYSW
                     LPLGYKVLRRIETIVREEMDAIGAQEVHFPALLPREPYEASNRWTDYGPNIFRLEDRK
                     GADYLLGPTHEEMFTLLVKDLYSSYKDLPLSLYQIQTKYRDEARPRAGILRGREFVMK
                     DSYSFDVSDEDFLKSYEAHRHAYIRIFDRLGFDYVIVAAMSGAMGGSASEEFLVKAEN
                     GEDTYVRCTNCDYAANVEAVRVPVPEPASYADAPEAHAEHTPDTPTIETLVDHLNQKF
                     ARGDREWSAGDTLKNVVVTLVHPDGHREPLSIGIPGDRDVDLKRLGAQVEPAEVEAFD
                     ERDFAKYPTLAKGYIGPGALGEKGTSGIRYVLDPRVVSGTRWVTGANQDGHHVLDLVA
                     GRDFEGDGWIEAADVRDGDPCPKCGHALETARGIEMGHIFQLGRRFAEVLDLRVLDES
                     GKLVTVTMGSYGVGISRAVAALVENSHDELGIIWPREVSPADVHLVATGKDAAVFDAA
                     DRLCTELESRGVSVLYDDRQKVSPGVKFTDAELIGVPTIVVVGKGLADGVVEVRDRRS
                     GDRENVAVTEASEHVARVVRGD"
     gene            3743..4438
                     /locus_tag="VH419_14925"
     CDS             3743..4438
                     /locus_tag="VH419_14925"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEY7044960.1"
                     /translation="MAIDAVIFDWGGTLTPWRTISPDEEWASLAAVAAPDRRTAVTDA
                     LVAAATSVWARAREEHRSATIEEICRLADLVYDVEHLQAYRDFWEPATWTHEDVPTLF
                     NWLRGEGIKIGVLSNTVWPRDWHEEIFVRDGVDHLIDGAVFTSEIEWTKPAPQAFEAA
                     MHAVGATVPERCVFVGDRLFDDIYGAKNVGMKAVLLPHSDIPVDQHGHTLGEPDAVIQ
                     RLADLPDVLSPWR"
     gene            4610..5344
                     /locus_tag="VH419_14930"
     CDS             4610..5344
                     /locus_tag="VH419_14930"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY7044961.1"
                     /translation="MEVIVMPVALITGGSRGLGREIALALAGRGWHLVTDARTPTPLE
                     AAADELRAAGAASVTAVAGSVAEADHRDRLSAAVAETGALDLLVNNASLLGPSPMPLL
                     AEYPLDTFVQVYAVNVVAPLAITQQLLPRLRASRGRLLNITSDAAVEPYDGWGGYGSS
                     KAALDQWTAIFAAEHPDLHVYALDPGDMRTTMHQDAFPGEDISDRPEPRTVVPAVLRL
                     VDDDLPSGRYRAAELLAGSAQAGEVA"
     gene            5341..6411
                     /locus_tag="VH419_14935"
     CDS             5341..6411
                     /locus_tag="VH419_14935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HEY7044962.1"
                     /translation="MSTAVATPIRFHVPPDSEATAPAERRGIARDGVRLLVASPGRIV
                     HAHFRDLADHLQPGDLVVVNTSATLPAAVDGVRRTGEHTIVHVSTELDDGMWVIEVRR
                     PDNSGPADDVAPGERFELPGGLRLTAVESFPEGGVSGARLWRAITSTRLDRTQFLADH
                     GRPIRYGYLSARWPLGDVQNVYAVQPGSAEMPSAGRPFTDRLLVRLMSRGVIVAPLVL
                     HTGVSSPEKHEPPAPEWYDVPTATADLVNLTRAAGRRVVAVGTTVTRALESAVDPDGI
                     VTAQQGWTDLVLGPGRPVRVVTGLVSGLHEPEASHLLLLEAVAGTSLVGRAYREAVAR
                     RYLWHEFGDSMLFLPACPAAAH"
     gene            complement(6424..6855)
                     /locus_tag="VH419_14940"
     CDS             complement(6424..6855)
                     /locus_tag="VH419_14940"
                     /inference="COORDINATES: protein motif:HMM:NF025883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEY7044963.1"
                     /translation="MTAVDALQECLAAEHAAVYGYGVLGGVLAGVTPVGSSEQQRAAS
                     AYVEHRRRRDELTSLITGRGSEPVAAEAAYDVPRQVQTVQDCRDLARLIEDRCAQTYA
                     DAVSRTTDRDRELTAGALTACTLTAVGWGAPIEPFPGVAEI"
     gene            complement(6852..7358)
                     /locus_tag="VH419_14945"
     CDS             complement(6852..7358)
                     /locus_tag="VH419_14945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044964.1"
                     /translation="MTTPQPSRRGVLTSFVVALPLFLSGCGRDAGRRRTARATSLSAA
                     DDADVVLVSAAVADESALLSYSVRSGRAHPELASVVAPITARQRVHVRELTSVVTELP
                     EPVPAGRPDVPGSRQAALTKLRRLVAAAEQARLDDSLAAESGLLARLLASASASHAAT
                     VESLRRTP"
     gene            7503..8000
                     /gene="rimP"
                     /locus_tag="VH419_14950"
     CDS             7503..8000
                     /gene="rimP"
                     /locus_tag="VH419_14950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004808687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEY7044965.1"
                     /translation="MTGHQPPQALIDVLEAPLARSGLDLEGVEMSRVGRRNLVRVLVD
                     QDGGVTLDAIADATHLVSQVLDESDVLGDSPYTLEVTSPGTDRPLSHARHWHRNVGRL
                     VKVTPKDGDTFVGRVREADEDGVVLDVEGASRRIPYADVSTARVQVEFNRPKSQQHAQ
                     PVEEG"
     gene            8004..>8963
                     /gene="nusA"
                     /locus_tag="VH419_14955"
     CDS             8004..>8963
                     /gene="nusA"
                     /locus_tag="VH419_14955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020387319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HEY7044966.1"
                     /translation="MDIDMTVLRSLVAEKEISLDVLVDAIEQALLVAYHRTPGAFEDA
                     RVELDRKTGHVTVYAAERDDDGEIVREFDDTPEGFGRIAATTAKQVILQRLRDAEDDI
                     KFGEFSGKEGDIISGIVQQGRDPEMVFVDLGKIEAVLPAAERVPGERYDHGRRIRCLV
                     VSVRKGPKGPQVMVSRSHPNLVKKLFALEVPEIADGTVEIAGIAREAGHRTKIAVRSK
                     SENVNPKGACIGPMGQRVRNVMNELHGEKIDIVDWSEDPAEMIAHALSPATVSSVEIV
                     DEAARSARVVVPDFQLSLAIGKEGQNARLAARMTGWRIDIRSDT"
BASE COUNT         1453 a         3040 c         3103 g         1367 t
ORIGIN      
        1 cggagtaccg gcgagcgctg ctcgtcgcag ccggcgtgct gctggcggcg ggctgcacca
       61 tccagggaga ttcggacgcg agtccgaact caccgtcgac cggctcgccc cctgttgacg
      121 cgacgccctc ctcgccaagc acatcagcga ccacccagtc gaccgagcca accgccccga
      181 ccgctccgac ttccgcgacc gagacggctg agccgcggca gatcaccgtg gtcatgaacg
      241 gcgacatgct gctgcacgag ggtctctggg agtccgcgca gatcgacgcg gcacgcaccg
      301 gcagaggtcc ggacgggatg gacttccggc cgatcctcgc cgacatgcga ggtgtggtgt
      361 ccggcgccga cctggcggtc tgccatctgg agacaccgct cgcgccccac ggcggaccgt
      421 actccggcta tccggtcttc tccgtcccgc cggccatcct gccggccctg aagtgggagg
      481 ggtacgacgc ctgtacgacg gcgtcgaacc acagtctcga ccagggcttc gacgggttga
      541 agaggaccat cgacgacttc gaccggattg gtcttgcgca tgccggcacc gctgagacgc
      601 agaaggcctc gcgtcagccg ctgctgctgg aggtgaaggg cgtcaaggtc gcgctgatct
      661 cggcgacgta cgggacgaac gggatcccgc ttccggccga cgagccgtgg tcggtgccgc
      721 tcatcaaccc cgaccggatc gagcggctcg cgcacagggc ccggtcggag ggtgccgaga
      781 tcgtgctggt cgccttgcac tgggggctgg agtacatgca tgcgccgacg tccgaccaga
      841 tcgcgatcgc cgacgagctg acccagtccc cggacatcga cttcgtgtac ggccaccacg
      901 cccacgtcgt ccagccgtac gaccgcatcc acggcacctg ggtcgtctac ggcctcggca
      961 acgcggtggc gcagcaagac actgccgtcg agggcgtgta cgacggcaac acctgccggg
     1021 tgacgttcac cgaacagccc gacggatcgt tcaaggtgag ccggctggag tacatcccga
     1081 cgatgatcac cccattcgac ggcgtccacc cgatgcgctg gttgaacgtg ccgaaggaca
     1141 tccacgaccc ggcgtacgcc ggtctgcgac ccgagctcga ggccacccag ggccgggtcc
     1201 ggaccgtcgt cggctccctc ggcgccttca agcacggcgt caccgaaggc cactgacccc
     1261 gccgcctctc gccgaggtgg caagtttggt tcgcttctgg cggcgtgtcg gacggcgact
     1321 gccacctcgg ccgtcaccgc ttcgagacct tggcggtcga accaccgacg ggtgtgcgac
     1381 gtaccctcag tatgaggaca agacggtgtt gatgatggtg ccgtggatcg tgaccatcgc
     1441 agcagccctg gtgttcgtcg ggctgctcga ctgcgtgctg cccaagcgcc ggacccactg
     1501 gacggtccgg ctcggcaaca acctgcgcgg tctggtgtcg cggctgcgac ggtcccgcgt
     1561 gaccgaccct gatccgttcg tcgtgctgcg catccagagt cgtctcggtg tgctggcttc
     1621 ggagatccgc gctgtcgagg cggaccggtt cgcatacgcg aaggtgcgtc ggctcgaggc
     1681 gctccaggcg gcgtacgacg acctgctcaa cgaaggctgc aaactggcag gcgtcgacgc
     1741 gttcgagacc caggaccgcg aggccaagcg gtggcgcgaa gagcaggagc tggccgagcg
     1801 cggctggtcc tggtagcgag ggatcgccac cgaggtggca acaccgtgac gacacgccga
     1861 caaactccaa cgattgttgc cacctcggcg tacacaggaa actggcgtga ggtggtgggg
     1921 gtgtggcgga tatcctcgga ggccacgtcg gcgaaccgaa gccgtcccga ccagcaggag
     1981 agacccgtgt ccatgcggat gtcgtcgctg ttccttcgta ccctgcgcga ggacccggcg
     2041 gacgcggaga tccccagtca caagctgctc gtccgcgccg gctacatccg ccgggcggcg
     2101 ccgggcatct actcctggct cccgctggga tacaaggtcc tccgcaggat cgagaccatc
     2161 gtccgggagg agatggacgc aatcggcgcg caggaggtgc atttcccggc gctgctcccg
     2221 cgtgagccct acgaggcgag caaccgctgg acggactacg gtcccaacat cttccggctc
     2281 gaggaccgca agggggccga ctacctgctc ggtccgacgc acgaggagat gttcacgctg
     2341 ctcgtgaagg acctctactc gtcgtacaag gatctgccgc tgtcgctgta ccagatccag
     2401 accaagtacc gcgacgaggc gcggccgcgg gccgggatcc tgcgcggccg cgagttcgtg
     2461 atgaaggact cgtactcctt cgacgtgtcc gacgaggact tcctgaagtc ctacgaagcc
     2521 catcgtcacg cctacatccg gatcttcgac cggctcgggt tcgactacgt gatcgtggcg
     2581 gcgatgtctg gcgccatggg cggctcggcc agcgaggagt tcctcgtcaa ggcggagaac
     2641 ggcgaggaca cctacgtccg ctgcacgaac tgcgactacg ccgccaacgt cgaagccgtc
     2701 cgtgtgccgg ttccggagcc ggcgtcgtac gccgacgcgc cggaagcgca cgccgagcac
     2761 acgcccgaca cccccacgat cgagacgttg gtcgaccacc tcaaccagaa gttcgctcgc
     2821 ggcgaccggg agtggagcgc gggtgacacg ctgaagaacg tcgtcgtgac gctcgtccac
     2881 ccggacggtc accgtgagcc gctgtcgatc ggcatacccg gcgaccgcga cgtcgacctg
     2941 aagcggctcg gggcgcaggt cgagccggcc gaggtcgagg cattcgacga gcgcgacttc
     3001 gcgaagtacc cgacgctggc caagggctac atcggccccg gtgcgctcgg cgaaaagggc
     3061 accagcggca tccgctacgt cctcgacccc cgcgtggtca gcggaacgcg ctgggtcacc
     3121 ggcgccaacc aggacggtca ccacgtgctc gacctggtgg ccggccgtga cttcgagggt
     3181 gacggctgga tcgaggcggc cgacgtccgg gacggtgacc cgtgtccgaa gtgcggccac
     3241 gccctcgaga cggcgcgcgg catcgagatg ggccacatct tccagctcgg ccgccggttc
     3301 gccgaggtgc tcgacctcag ggtgctggac gagagcggca agctcgtcac ggtgacgatg
     3361 ggctcgtacg gcgtcggcat ctcccgggcg gtcgccgccc tcgtggagaa ctcacacgac
     3421 gagctcggca tcatctggcc ccgtgaggtc tctccggcgg acgtgcacct ggtcgcgacc
     3481 ggaaaagatg cggcagtctt cgatgctgcc gatcggctct gcaccgagct cgagtcgcgc
     3541 ggggtcagcg tgctctacga cgaccggcag aaggtctcac caggtgtgaa gttcaccgat
     3601 gccgagctga tcggtgtccc gaccatcgtc gtggtgggca agggactcgc cgacggtgtg
     3661 gtcgaggtcc gcgaccggcg cagcggcgac cgcgagaatg ttgccgtcac cgaggcgtcc
     3721 gagcacgtcg ccagggttgt ccgtggcgat tgacgccgtc atcttcgact ggggtggcac
     3781 gctcacaccg tggcgcacga tctcaccgga cgaggagtgg gcgtcgcttg ctgccgttgc
     3841 cgcgccagat cggcgtactg ccgtgacaga tgcactcgtc gcggcagcca cgtcggtttg
     3901 ggcgcgcgcc cgtgaggagc accgcagcgc cacgatcgaa gagatctgtc gcctcgccga
     3961 cctggtgtac gacgtcgaac acttgcaggc ataccgcgac ttctgggaac ccgcgacgtg
     4021 gacccacgag gacgtgccga cactgttcaa ctggctgcgc ggcgagggca tcaagatcgg
     4081 tgtcctgtcc aacaccgtat ggccgcgtga ctggcacgag gagatcttcg tccgcgacgg
     4141 ggtcgaccat ctcatcgacg gtgcggtgtt cacttccgag atcgagtgga ccaagccggc
     4201 gccgcaggcg ttcgaggcgg ccatgcacgc ggtcggtgct acggtcccgg agcggtgcgt
     4261 cttcgtcggt gaccggttgt tcgacgacat ctacggcgcg aagaacgtcg ggatgaaggc
     4321 cgtgctgctg ccacacagcg acattcccgt cgaccagcac ggccacacgc tcggggaacc
     4381 cgatgcggtc atccagcggc tggccgacct cccggacgta ctttccccct ggcgctagcg
     4441 ggggcttccc ggaattgcgg gaaaccggcg gcccataccg gcgtgtgagc cgccactttc
     4501 ccgcaattcg cgggaaccag gcagcggccg gaagccgaac cggacttgac ctcaagttaa
     4561 cttgaagtag cacgatggcg ggactcgaac aacaacgaga cacctgatcg tggaggtcat
     4621 cgtcatgccg gttgccttga tcaccggagg gtcccgcggg ctcggtcggg agatcgctct
     4681 cgccttggcg ggacgtggct ggcacctcgt cacggacgcg cggacgccca ctccgctcga
     4741 ggccgctgcg gacgagctgc gagcggcagg agcggcctcg gtgacagccg tagcaggaag
     4801 cgtcgccgag gccgaccatc gtgaccggtt gtcggcggcc gtcgcggaga ccggcgcact
     4861 cgacctgctc gtcaacaacg ccagcctgct cggccccagc ccgatgccgc tcctcgcgga
     4921 gtacccgctg gacacgttcg tgcaggtgta tgccgtcaac gtcgtcgcgc cgctcgccat
     4981 cactcagcag ctgttgccgc ggttgcgcgc ctcccgtggg cgactgctca acatcacgtc
     5041 cgacgccgcg gtcgagccgt acgacggctg gggcggctac ggatcgtcga aggcggcgct
     5101 cgaccagtgg accgccatct tcgccgcgga gcacccggac ctgcacgtct acgcactcga
     5161 cccgggcgac atgcggacca cgatgcatca agacgcgttc cccggcgagg acatttcgga
     5221 ccgaccggag ccgcggacgg tcgtgccggc cgttctccgg ctcgtcgacg acgacctgcc
     5281 gagcggccgc taccgagccg ccgagctgct ggcgggttcg gcacaggcgg gagaggtcgc
     5341 gtgagcacgg cagtcgccac gccgatccgg ttccacgtgc cgcccgacag cgaggcaacc
     5401 gcaccagccg agcggagggg aatcgcccga gacggtgtgc ggttgctcgt cgcctcgcca
     5461 ggacggatcg tgcatgcgca cttccgtgac ctcgccgacc acctccagcc aggcgacctc
     5521 gtggtcgtca acacctcagc aacgttgcct gcagcggttg acggtgtgcg gcgtaccggc
     5581 gaacacacga tcgtgcacgt gtcgaccgag ctcgacgacg gcatgtgggt catcgaagtc
     5641 agacgtcccg acaacagtgg tccggccgac gacgtggctc cgggggagcg cttcgagctg
     5701 ccgggaggtc tacgcctgac ggccgtcgag tcgtttcccg agggtggtgt gtctggtgcc
     5761 cggttgtggc gcgccatcac ctcgacgcgg ctcgaccgga cgcaattcct cgccgaccac
     5821 ggccgaccca tccgctacgg ctacctcagc gcacgctggc cgctgggcga cgtccagaac
     5881 gtctacgcgg tccagcccgg cagtgccgag atgcccagcg ccggccgccc gttcaccgat
     5941 cgcctgctgg tgcggctgat gtcgcgtggg gtgatcgtgg cgccgctggt cttgcacacc
     6001 ggcgtgtcga gcccggagaa gcacgaacct cctgcaccgg agtggtacga cgtgcccacc
     6061 gcaaccgcgg acctggtcaa tctcacgcgg gcggcaggcc gtcgcgtcgt cgccgtcggc
     6121 accacggtga cccgtgcgtt ggagtcagct gtcgacccgg acggcatcgt caccgcacag
     6181 cagggctgga ccgatctcgt gctcgggccg ggccgtcctg tccgcgtggt cactggcctg
     6241 gtgagcggcc tgcacgagcc ggaggccagc catctgctgt tgctcgaggc cgttgccggt
     6301 acgtcgctcg tcggacgcgc gtaccgggag gcggtggctc gtcgctacct ctggcacgag
     6361 ttcggcgact cgatgctctt cctcccggcg tgcccggccg ctgcccattg acgccgaccc
     6421 gagtcagatc tcggcgacac cggggaacgg ctcgatgggt gctccccagc ccacggctgt
     6481 gagcgtgcat gcggtcaacg cgccggcagt gagctcccgg tcgcggtcgg tggtcctgga
     6541 cacggcatcg gcgtaggtct gcgcgcacct gtcctcgatg agccgcgcca gatcccggca
     6601 gtcctggacg gtctgcacct gcctgggcac gtcgtacgcc gcttcggctg cgaccggttc
     6661 gctgcctcga cctgtgatga gcgaggtgag ctcgtccctg cggcggcggt gctcgacgta
     6721 cgccgaagcc gcccgctgct gctcggagga gccgacgggg gtcacacccg cgagcacccc
     6781 gccgaggacc ccgtaaccgt agacggccgc atgctcggcg gccaggcact cctgcagggc
     6841 atcgacggcg gtcatggcgt cctgcgcagg gattccacgg tggcggcgtg cgaggccgat
     6901 gccgatgcga ggagccgggc gagcaggccg gattcagcgg ccaggctgtc gtcgagccgg
     6961 gcctgttccg ccgcggcgac gaggcgtcgc agtttcgtga gtgccgcctg tctggagcca
     7021 ggtacatcag gacggccggc gggcaccggc tctggcagtt cggtgaccac ggacgtgagc
     7081 tcacggacat ggacgcgttg gcgtgccgtg atcggcgcga cgacgctcgc gagctcggga
     7141 tgtgcccgtc cgcttcgcac cgagtacgac agcaacgccg actcgtctgc gacggctgcg
     7201 ctgacgagga cgacgtccgc gtcgtcggcg gcgctcaagc tggtcgcccg cgccgtacgc
     7261 cgccggccgg cgtcacgtcc acagcctgag aggaagagcg gaagcgccac cacgaaggag
     7321 gtcaggacac cgcggcgcga gggctgcggg gtcgtcacgc gagcgaccgt agcaggtacg
     7381 ggtgtgcggt ccggaacgtg agagcatgcc tgtccggcgg tggttcggta gatcctgcga
     7441 tcggccgcgt tcgtctagtc tgagagggcg cgaggacaac accacaggga ggaggacggt
     7501 ccgtgaccgg tcaccagccc ccacaagccc tgattgatgt gttggaagct ccgctggcga
     7561 ggtccggcct cgatctcgaa ggcgtcgaga tgtcgcgtgt cggtcgccgc aacctggtgc
     7621 gcgtgctcgt cgaccaggac ggcggcgtca ccctggacgc catcgccgac gccacgcatc
     7681 tggtgagcca ggtgctggac gagtccgacg tgctcggcga ctcgccgtac acgctggagg
     7741 tcacgtcacc cggcaccgac cgaccgctga gtcacgcccg ccactggcat cgcaacgtcg
     7801 ggcgactggt caaggtcacg ccgaaggacg gcgacacgtt cgtcggtcgt gtccgcgagg
     7861 ccgacgaaga cggcgttgtc ctggacgtcg agggtgcctc gcgtcggatc ccgtacgccg
     7921 acgtctccac cgcccgagtg caggtcgagt tcaaccgtcc gaagtcgcag cagcacgctc
     7981 agcctgtcga ggagggatga ccggtggaca tcgacatgac ggtgctgcgt tcgctggtcg
     8041 ccgagaagga gatctccctc gacgtgctcg tcgacgcgat cgaacaggcg ttgctggtgg
     8101 cgtaccaccg cacgcccggc gccttcgagg acgcgcgggt cgaactcgac cgcaagaccg
     8161 gtcacgtgac ggtgtacgcc gccgagcgcg acgacgacgg tgagatcgtt cgcgagttcg
     8221 acgacacacc ggaggggttc ggccggatcg ccgccaccac cgccaaacag gtcatcctgc
     8281 agcggctgcg cgacgccgag gacgacatca agttcggcga gttctccggc aaggaaggcg
     8341 acatcatctc cgggatcgtc cagcagggac gtgatcccga gatggtcttc gtggacctcg
     8401 gcaagatcga ggcggtgctt cccgcagccg agcgtgtgcc gggggagcga tacgaccacg
     8461 gtcggcgcat ccgctgcctc gtcgtgtcgg tccgcaaggg tccgaaggga ccgcaggtca
     8521 tggtgtcgcg gtcccacccg aacctggtca agaagctgtt cgccctcgag gtccccgaga
     8581 ttgcggacgg gaccgtcgag atcgccggca tcgcccgcga ggccggccac cgcaccaaga
     8641 tcgcggtccg gtcgaagtcg gagaacgtca acccgaaagg ggcctgcatc ggcccgatgg
     8701 gccagcgagt ccgcaacgtc atgaacgagc tgcacggcga gaagatcgac atcgtcgact
     8761 ggtcggagga cccggccgag atgatcgcgc acgcgctctc gccggcgacc gtgtcgagcg
     8821 tggagatcgt cgacgaggcg gcccggtcgg cgcgtgtcgt cgtaccggac ttccagctgt
     8881 cgctcgcgat cggcaaggag gggcagaacg cccggttggc cgcgcgtatg acgggctggc
     8941 ggatcgacat ccggtcagac acc
//
LOCUS       DATBFZ010000490         4775 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_3795960, whole genome shotgun sequence.
ACCESSION   DATBFZ010000490 DATBFZ010000000
VERSION     DATBFZ010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 4775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4775
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3795960"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="VH419_14960"
     CDS             <1..387
                     /locus_tag="VH419_14960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008356537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HEY7044967.1"
                     /translation="STNILPVGDPDNVATAWKLHARLVRRHLERGIYQGWDMHPAQLV
                     TRFLATYAFYRGAFVPAATRLRNYVHRLDSTVMDEPATARALASVIHRGSVCGALTVD
                     EIETATDLSMSTVRDIALGRPIRSTP"
     gene            384..1211
                     /locus_tag="VH419_14965"
     CDS             384..1211
                     /locus_tag="VH419_14965"
                     /EC_number="3.5.3.26"
                     /EC_number="3.5.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008364285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004037 - allantoicase activity [Evidence
                     IEA];
                     GO_function: GO:0071522 - ureidoglycine aminohydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0010136 - ureide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional allantoicase/(S)-ureidoglycine
                     aminohydrolase"
                     /protein_id="HEY7044968.1"
                     /translation="MTHPVRQASYYTPRGGLPPQTDLLTDRAIVTEAYTVIPRGVLSD
                     IVTSVFPEWTDTRAWIINRPVAGGATTYYQAIVEVKPGGGAQRPEPQPEVQSFLFVTS
                     GALTVGAAGQSQTLTEGGFAYLPAGTDWSAHNNGDADATFVWIRKRYEAIEGHTASVK
                     FGNEQDIEPSAMPGTDGKWRTTRMLDPQDLAYDMHVNVVTFEPGATIPFAETHVMEHG
                     LLMLEGKAVYHLNGDWVEVQEGDFLSLRAFCPQACYAGGPSNFRYLLYKDVNRQIML"
     gene            complement(1266..1988)
                     /locus_tag="VH419_14970"
     CDS             complement(1266..1988)
                     /locus_tag="VH419_14970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009079571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HEY7044969.1"
                     /translation="MRILVANVNTTVSMTDAIAESARGVASPGTEIVGITPRFGADSC
                     EGNFESYLAAIAVMDAITSYPEPFDAVIQAGYGEHGREGLQELLDVPVVDITEAAAST
                     AMYLGHKYSVVTTLDRTVPLIEDRLKLAGLDARCASVRASGLGVLELESDPDRAVEAI
                     VRQSREAVENDHAEVICLGCGGMAELEAKVRAATGVPIVDGVRSAVTIAEGLVRMGLS
                     TSKVRTYATPREKKVIGWPFQL"
     gene            complement(2001..3506)
                     /locus_tag="VH419_14975"
     CDS             complement(2001..3506)
                     /locus_tag="VH419_14975"
                     /inference="COORDINATES: protein motif:HMM:NF014218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS1 family nucleobase:cation symporter-1"
                     /protein_id="HEY7044970.1"
                     /translation="MSTESTGLIGDTSRNDATPFADVSPHLYNADLAPTKREGRRWGA
                     YNIFTLWANDVHSLGNYSFAIGLFALGLGGWQILLVLGLGGAFLFLLLSLSGFMGEKT
                     GVPFPVMSRISFGIRGAQIPAIIRGAVAIAWFGIQTYLASVVLRILIVALAPSAATLD
                     SNSFLGLSTLGWISFVGLWVIQVVIVSYGMEMIRKYEAVAGPVILITMVALAVWMLSS
                     ADWSIAWTTPDSLTGVDMWLKIVGGASLWVAIYGTFVLNFCDFTRNATSKRAIVKGNF
                     WGIPLNMLVFGSIVITLAGAQFRIDGRIIESPADIVKEVPNTLLLVLAGLALLILTIA
                     VNLMANFVAPIYALNNLFPKHLNFRRAALISAVIGLVILPWNLYNSPAVINYFLGGLG
                     AILGPLFGIIMADYWLVRRSKINVPALFSTDALGEYHYAKGVNVRAVIALVITAVVAL
                     LLAFVPAFRVVSEFSWFIGAGLGAIVYLAVADRRGPFNDVCGEPIAVASTH"
     gene            3731..>4775
                     /gene="allB"
                     /locus_tag="VH419_14980"
     CDS             3731..>4775
                     /gene="allB"
                     /locus_tag="VH419_14980"
                     /EC_number="3.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008364286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0050897 - cobalt ion binding [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HEY7044971.1"
                     /translation="MTADAPETLNHPEFDLVVRGERTLTTAGIVAREIGIRDGRVVAI
                     EPLGSGLTGAEIIELTDEQVMIPGLVDTHVHVNEPGRTEWEGFDSATRAAAAGGVTTL
                     IDMPLNSIPPTVNVEALNAKRAAASGKTHIDVGFWGGAIPGNTDDLRGLHDEGVFGFK
                     CFLLHSGVDEFPHLDADEMEKDMAVLADFDSMMIVHAEDSRAIDHAPTAEGDRYERFL
                     ASRPRGAENVAIAEVIERARWTGARAHILHLSSSDALPMLATAKRDGVKISVETCPHY
                     LTLLAEEIPNGATAFKCCPPIREASNRELLWQGLLDGTIDCIVSDHSPSTVDLKDVEN
                     GDFGVAWGGVASLQ"
BASE COUNT          885 a         1575 c         1536 g          779 t
ORIGIN      
        1 tcgacgaaca tcctcccggt cggcgacccc gacaacgtcg ccacagcgtg gaagctgcac
       61 gcgcgcctag tccggcgtca cctggagcgc ggcatctacc aaggttggga catgcacccc
      121 gcccaactcg tcacccgatt cctggcgacc tacgcgttct accgtggcgc cttcgtgcca
      181 gccgcgacac ggctacgcaa ctacgtccac cggctcgact cgaccgtcat ggacgaaccg
      241 gccaccgccc gcgccctcgc cagcgtgatc catcgtggca gcgtgtgtgg cgccctgacc
      301 gtcgacgaga tcgagacagc caccgatctg tccatgtcca ccgtgcgcga catcgcgctc
      361 ggccgcccta tcaggagcac cccgtgaccc acccagtgag gcaagcgtcg tactacacgc
      421 cccgcggcgg cctgcccccg cagaccgatc tgctgaccga tcgcgccatc gtcaccgagg
      481 cctacacggt gatcccgcgc ggagtgctct cggacatcgt cacctcggtg ttcccggagt
      541 ggaccgatac gcgcgcatgg atcatcaacc ggccggtcgc cggtggtgcg acgacgtatt
      601 accaggcgat cgtcgaggtc aagccgggcg gcggggcgca gcgacccgag ccgcaacccg
      661 aggtgcagag cttcctgttc gtcacgtccg gtgcgctcac tgtgggcgcg gcaggccagt
      721 cgcagacact caccgagggc ggcttcgcgt accttccggc aggcaccgac tggtcggccc
      781 acaacaacgg cgacgccgac gcgacgttcg tgtggatccg caagcgctac gaggccatcg
      841 agggccacac ggcatcggtg aagttcggca acgagcagga catcgaaccg tcggcgatgc
      901 ccggaaccga cggcaagtgg cgcaccacac gcatgctcga cccgcaggac ctcgcctacg
      961 acatgcacgt caacgtcgtc accttcgagc cgggcgcgac cattccgttc gccgagaccc
     1021 acgtgatgga acacggcctc ctgatgctcg agggcaaggc cgtctatcac ctcaacggag
     1081 actgggtgga ggttcaggag ggcgacttcc tgtcgctgcg tgccttctgc ccgcaggcgt
     1141 gttacgcggg cgggccgtcc aacttccgtt acctgctgta caaggacgtc aaccgccaga
     1201 tcatgctctg acgcgtagaa ccgggcgcct ctttcgaggc gcccggtctc gtctatttcg
     1261 ttggcctaca actggaatgg ccagccgatg acctttttct cacgcggagt cgcgtaggta
     1321 cgcaccttcg acgtggacag gcccatccgg accagccctt cggcgatggt gaccgccgaa
     1381 cggactccgt cgacgatcgg gacgccggtc gcggccctca ccttcgcctc gagttcggcc
     1441 ataccgccgc agccaagaca gatcacttcg gcgtgatcgt tctcgaccgc ctctcgcgat
     1501 tggcggacga tcgcttcgac cgcccgatcc ggatccgact cgagttcgag caccccgagg
     1561 cccgacgccc gcaccgacgc gcatcgtgcg tcgagtcccg cgagcttgag acggtcttcg
     1621 atcagcggga cggtgcggtc gagcgtggtg acgaccgagt acttgtgtcc cagatacatc
     1681 gccgtcgacg cggccgcctc ggtgatgtcg accaccggga cgtcgagcaa ctcctgcaga
     1741 ccttctcggc cgtgctcgcc gtagccggcc tggatcaccg cgtcgaacgg ctcggggtac
     1801 gacgtgatcg cgtccatgac cgcgatggcc gcgagatagc tctcgaagtt tccttcacac
     1861 gagtccgcgc cgaagcgcgg tgtgatgccg acgatctcgg tgcccggaga cgcgacaccg
     1921 cgcgcggact cggcgatcgc atcggtcatc gagaccgtcg tgttgacgtt ggcgacgagg
     1981 attctcatgg cggtccctgt ctagtgcgtg ctcgccacgg cgatgggttc gccgcagaca
     2041 tcgttgaacg gccctcggcg gtctgcgacc gccaagtaca cgatcgcacc gagtcctgcg
     2101 ccgatgaacc aggagaactc cgagacgacc ctgaacgccg gaacgaacgc gagcaggagt
     2161 gccaccaccg cagtgatgac gagggcgatc acggcacgca cgttgacgcc cttcgcgtag
     2221 tggtattcac cgagcgcatc ggtcgagaac agggcgggaa cgttgatctt ggaacgacgc
     2281 accagccagt agtcggccat gatgatgccg aacagcggcc cgagtatggc gccgaggccg
     2341 ccgaggaagt agttgatgac ggcaggggag ttgtacaggt tccacggcag gatcaccagg
     2401 ccgatgacgg ccgagatgag tgctgcgcga cggaagttga ggtgcttcgg gaacaggttg
     2461 ttgagcgcat agatcggcgc gacgaagttc gccatcaggt tcaccgcgat ggtcaggatc
     2521 agcagcgcga gcccggcgag caccagcagc agggtgttcg gcacttcctt gacgatgtcg
     2581 gcgggcgatt cgatgatgcg tccgtcgatg cggaactgag caccggccag agtgatgacg
     2641 atgctgccga acaccagcat gttgagcggg atgccccaga agttgccttt cacgatggcc
     2701 cgcttcgacg tggcgttccg ggtgaagtcg cagaagttga ggacgaacgt gccgtagatc
     2761 gcgacccaca ggctggcgcc gccaacgatc ttcagccaca tgtcgacgcc ggtcagcgag
     2821 tcgggcgtgg tccacgcgat ggaccagtcg gcgctgctga gcatccagac cgcgagcgcg
     2881 accatcgtga tcaggatgac cggacccgcg accgcctcgt acttgcggat catctccatg
     2941 ccgtaactga cgatgaccac ctggatcacc catagcccga cgaacgagat ccagccgagc
     3001 gtcgacaacc ccaggaagct gttcgagtcc agggtcgccg ccgacggtgc gagcgcgacg
     3061 atcaagatgc gcagcaccac ggacgcgagg taggtctgga tgccgaacca tgcgatcgca
     3121 acggcgccgc ggatgatcgc ggggatctgt gcgccgcgga tgccgaagga gatgcggctc
     3181 atcaccggga acgggacgcc ggtcttctca cccatgaacc cggacaggct cagcagcagg
     3241 aagaggaatg cgccgccgag tccgagcacc agcaggatct gccacccacc gaggccgagg
     3301 gcgaacaggc cgatggcgaa cgaatagttc cccaggctgt gtacgtcgtt cgcccacaag
     3361 gtgaagatgt tgtacgcgcc ccaccgtcga ccttcgcgct tggtgggcgc gagatcggcg
     3421 ttgtacagat gcgggctgac gtcggcgaac ggggtcgcat cgttacgact ggtgtcgccg
     3481 atgagcccgg tggactctgt gctcatggat gcgcctgtca cggtcggtgc gacggcgctc
     3541 gacgccggcg cagtattcca ttatgtggaa gtaatattcc acgagtttag taagtaagtc
     3601 gcatgtagac accggctgtc aagcctctcg gcccgattga ctgtggcaac gctcacattt
     3661 acagttgccc atagggtgga gtgttacttc cacgatgagg aattatgctg ctagtcatgg
     3721 agagctaccg atgaccgccg acgcacccga gacgctgaac caccccgaat tcgacctggt
     3781 ggtccgcggc gaacgcacgc tgacgacggc gggaatcgtc gcgcgcgaga tcggtatccg
     3841 cgacggtcgc gtcgtcgcga tcgagccgct ggggagtgga ctcaccggcg cggagatcat
     3901 cgaactcacc gacgagcagg tcatgatccc cggcttggtc gatacgcacg tgcacgtcaa
     3961 cgagccgggc cgcaccgagt gggaggggtt cgactccgct acccgcgcgg cggcggccgg
     4021 cggtgtgacc acgctgatcg acatgccgct caactcgata cccccgacgg tcaacgtcga
     4081 ggcactcaac gccaaacgtg cggctgcatc gggcaagaca cacatcgacg tcggtttctg
     4141 gggcggcgcg atccccggca acaccgacga tctgcgcggt ctgcacgacg aaggcgtgtt
     4201 cggcttcaaa tgcttcctgc tgcactccgg cgtcgacgag ttcccgcatc tggacgcgga
     4261 tgagatggag aaggacatgg ccgtgctggc cgacttcgac tccatgatga tcgtgcacgc
     4321 cgaggactcc cgcgccatcg accatgcccc cacagccgag ggggaccggt acgaacgctt
     4381 cctcgcgtcg cggccgcgcg gcgccgagaa tgtggcgatc gccgaggtca tcgagcgtgc
     4441 tcggtggacc ggtgcccgcg cgcacatcct gcacctgtcg tcgtcggacg ccttgccgat
     4501 gctcgccacc gcaaagcgcg acggcgtcaa gatctctgtc gagacgtgcc cgcactactt
     4561 gacgctcctc gccgaggaga tcccgaacgg cgcaaccgcg ttcaaatgct gtccgccgat
     4621 ccgtgaggcg tcgaatcggg aactgctgtg gcagggcctg ctcgacggca cgatcgactg
     4681 catcgtctcc gaccactcac cgtcgacggt cgacctgaag gacgtcgaga acggtgactt
     4741 cggtgttgcc tggggcgggg tcgcgtcgct gcagc
//
LOCUS       DATBFZ010000491         2311 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_4465115, whole genome shotgun sequence.
ACCESSION   DATBFZ010000491 DATBFZ010000000
VERSION     DATBFZ010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4465115"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="VH419_14985"
     CDS             complement(<1..325)
                     /locus_tag="VH419_14985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044972.1"
                     /translation="MHMKVAGVFTGRVTKWLTVVFWIAMLVALGPLAGKLTGIEDNEA
                     SSWLPASAESTQALKLQEKFQNTDVLPAIIVYERPSGITEQDKALAAQQAADLKNDQY
                     VVTDEV"
     gene            509..1432
                     /locus_tag="VH419_14990"
     CDS             509..1432
                     /locus_tag="VH419_14990"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY7044973.1"
                     /translation="MASEALASTPEPDTPTGADDAKATASGAGLPQLTGFLLRRAYVK
                     AAASAAACVGEDAHVRDVGLLAILDERGPLSQRHLSDMTRINPTIMVKVVDRLEQRGW
                     VVRERNPADRRSYAVRLTAEGSRAFSALSDELHIGERILTETLTTGQRDQLKRALLKL
                     LGGRPDPGIGSLSDHTGYLISLGHRLVRGWAIEALAPLDLDPRDFGVLSTIGRDQPCS
                     QNHLAQALGVTPPAALAFVEELASAGLVRRERNVGDRRSYDLTLTELGAERLAAARKA
                     AADVQTRVVDRLGEKADQRLRRLLTKVVYDA"
     gene            1874..>2311
                     /locus_tag="VH419_14995"
     CDS             1874..>2311
                     /locus_tag="VH419_14995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEY7044974.1"
                     /translation="MARAVGIDLGTTNSVVAVLEGGDPTVIANAEGSRTTPSVVAFAK
                     NGEVLVGEVAKRQAVTNVERTIRSVKRHMGTDWKQQIDDKTFTAQQISAFVLQKLKRD
                     AEAYLGETVTDAVITVPAYFSDAQRQATKEAGEIAGLNVQRIIN"
BASE COUNT          423 a          782 c          740 g          366 t
ORIGIN      
        1 ggacctcgtc ggtcacgacg tactggtcgt tcttgaggtc ggcagcctgc tgggcggcga
       61 gcgccttgtc ctgctcggtg atgccggacg gacgctcgta gacgatgatc gcgggaagga
      121 cgtcggtgtt ctggaacttc tcctggagct tcagcgcctg tgtcgactcg gcgctggccg
      181 gcagccagga cgacgcctcg ttgtcctcga ttccggtcag ttttcccgcc aacgggccaa
      241 gggcgacgag catcgcgatc cagaagacca ccgtcagcca cttcgtgacg cggccggtga
      301 acactccggc gaccttcatg tgcatggcgt tccctttccc gattcttcgt gcgaggctat
      361 gaacctggtc gcaaatcgtc aaccctatta atgatcaact tggtaaagtc aaggtcaaga
      421 tcagggaaac ccctgagccg cacgtgtaca gactggtgct gcaccgagaa ctgatcgggc
      481 tgcaccgaaa actgatcggg ctgggccgat ggcttctgaa gcgctggcgt cgacgcctga
      541 gcctgacacg cccacgggtg cggacgatgc caaggcgacc gcaagcggtg ccgggctgcc
      601 gcagctgacc gggttcctgt tgcggcgcgc gtacgtgaag gcggccgcca gtgctgcagc
      661 ctgtgtcggc gaggacgccc acgtcaggga cgtgggcctg ttggccatcc tcgacgaacg
      721 cggaccgctg tcccagcggc acctgtccga catgacccga atcaacccca cgatcatggt
      781 caaggtggtc gaccggctcg agcagcgcgg ctgggtggtc cgcgagcgca acccggcgga
      841 ccgtcgttcg tacgccgtgc ggctcacagc cgagggatcg cgagccttct ccgccctgag
      901 tgacgagctg cacatcggtg aacgcatcct caccgagacg ctcacgacgg gccagcgcga
      961 ccagctcaag cgcgcactgc tcaagctgct cggcgggcga cccgaccccg gcatcggctc
     1021 gctgtccgat cacaccgggt acctgatctc acttggacat cggctcgtcc gggggtgggc
     1081 gatcgaggca ctcgccccgc tcgacctcga cccacgtgac ttcggtgtgc tgtccacgat
     1141 cggccgtgac cagccctgct cgcagaacca cctcgcccaa gcactcggcg tgaccccgcc
     1201 ggccgccctg gcgttcgtcg aggagctggc gtcggcaggc ctggtgcggc gcgagcgcaa
     1261 cgtgggcgac cggcgctcgt acgacctcac cctcaccgag ctgggcgccg aacgcctcgc
     1321 cgccgcccgg aaagccgccg ccgacgtcca gacccgcgtc gtcgaccgcc tcggcgagaa
     1381 ggccgaccaa cgcctccgac ggctcctcac caaagtcgtg tacgacgcct gacctgggtc
     1441 gcttgcccgt gagggtttgc gatgtttaca gcccaaacgg cgtgtaaaca tcgcaaacct
     1501 ccgatggaca cagggggctt gggatgtgag catcgcaacc cttggccggg tggagcggcg
     1561 aaggtccgaa agccgccaac atgcgaccac caccagcggc ggaacccggt cgacaagctc
     1621 aggccccgca gtggctccgc tgtcgtcgcc gagaacccag ccgaagcaga cggacggtcc
     1681 gccgccgagc accgggggca cacgaacctg ccgatcgaag aaaccacccc cggatccggg
     1741 aagaaattgc cgcggcaggc gttgtatccg atgtatgact tgagtgtgcg cgactcaact
     1801 ttggagttgt cgccacgatc cggtagaact tgagtccaca aggctcaatc ttgactcgaa
     1861 aggtatgacc accatggctc gtgcggtcgg aatcgacctg ggtacgacga actccgtcgt
     1921 tgccgtcctc gagggagggg atcccaccgt catcgcgaac gccgagggct cccggaccac
     1981 tccgtccgtc gtggcgttcg ccaagaacgg tgaggtgctc gtcggtgagg tcgccaagcg
     2041 gcaggccgtc accaacgtcg agcgcaccat ccgctcggtg aagcgccaca tgggcaccga
     2101 ctggaagcag cagatcgacg acaagacgtt cacggcgcag cagatctccg cgttcgtcct
     2161 gcagaagctc aagcgggacg ccgaggcgta cctcggcgag accgtgaccg acgccgtgat
     2221 caccgtgccg gcgtacttca gtgatgccca gcgccaggcc accaaggagg ccggtgagat
     2281 cgccgggctc aacgtccagc gcatcatcaa c
//
LOCUS       DATBFZ010000492         1690 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_4793793, whole genome shotgun sequence.
ACCESSION   DATBFZ010000492 DATBFZ010000000
VERSION     DATBFZ010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4793793"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            465..1562
                     /locus_tag="VH419_15000"
     CDS             465..1562
                     /locus_tag="VH419_15000"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HEY7044975.1"
                     /translation="MDAVIARWRLCNQHLTAPHAVSVSEVMDKLLAVQAENPSQSAWA
                     VASRTVSPEPGRLAALLASGEVARTHVLRPTWHYVARGDIDWLLALTGPRLLKIIDAQ
                     LMGDLDLTTRNVQQLSAAVLDTLVATPDRTRQEVVAALRDQIPALAQRITGRLVMLLM
                     AHLELHRLVISGRPREGEHTYAAYEDRVGSRVDPARFDRDEALSKLALRYYTGHGPST
                     VKDLAYWATLTITDVRRGLDAVREHLDCFEHDGRTFWHAPGEPPAQPGKPAGHLLQLL
                     DEIYRGYQDSRWVLDASRVVPRQRESAIGIALIDAQLAAGMKRTLTASQVQFELTPYR
                     PLSSVEWEVLNEAASRYGTFLGRPATIVHSP"
BASE COUNT          278 a          574 c          561 g          277 t
ORIGIN      
        1 cggagttcca gtcgttgatg aggaccgggc tgaactgggc gcagatgttg ttcgcgttgc
       61 tgacgaagac cgcgttgtca tcgactacgg ggctcaccga ggttgtaatg ggcatcgtcg
      121 tgatgggcac cgtcgtgatg gtgaccgtcg tgctcggcgg gggcgtgggc gtcgtcgtgt
      181 caggtacggg cggtggagga tccggcgtgg gatcggagcc accacacgca cccagaacgg
      241 cgagcaccag caccactgcc accagggaaa cgtgtccgcc ccactcccgg ccattgatcg
      301 ccacagcaaa ccccccttgc caggccaatg ttcaatcgta ttgagtgcaa ctcgccatcg
      361 gcaagacacg cgccgcagct gactccgaag cgcggacaac cttcgggtcc gcatgtcgaa
      421 ggctgtccgg tctcttcgtc gtccgccgta cgatactggc gcgcgtggac gctgtgatcg
      481 cacgctggcg gctgtgcaac caacacctca ccgcgccgca tgccgtttcg gtgagcgagg
      541 tgatggacaa gctgttggcc gtgcaggcgg agaacccgag tcagtccgcc tgggcggtgg
      601 ccagccggac ggtctcgccg gagcctggcc gtctggccgc cctgctcgcc tccggggagg
      661 tggctcgtac gcatgtgctg cgcccgacat ggcactacgt ggcgcgcggt gacatcgact
      721 ggctgcttgc tctgaccgga ccaaggctgc tcaagatcat cgatgcccag ctgatgggtg
      781 acctcgacct gacgacccgc aacgttcaac agctgtccgc cgcggtcttg gacacgctcg
      841 tcgcgacgcc ggaccggacc cgccaggagg tcgtggccgc gttgcgcgac cagatccccg
      901 ccctggcgca gcggatcacc ggccggctgg tgatgctgct gatggcccac ctggagctgc
      961 accgtctggt catctccggc agaccgcgcg agggcgagca cacctacgcg gcatatgagg
     1021 accgggttgg ttcgcgagtc gacccggcgc ggttcgaccg tgacgaggca ctgtccaagc
     1081 tggcgctgcg ctactacacc ggtcacggcc cgtcgaccgt caaggatctc gcctactggg
     1141 ccaccctcac catcaccgac gtgcgccgtg gactggacgc ggtccgcgag cacctggact
     1201 gcttcgagca cgacgggcgc acgttctggc acgcgcccgg cgagcctccc gcgcaaccag
     1261 gcaaacccgc tggtcacctg ctgcagctgc tggacgagat ctaccgcggc taccaggact
     1321 cgcgctgggt cctcgacgcc agtcgcgtgg tcccgcgtca gcgcgagagc gcgatcggga
     1381 tcgcgctgat cgacgcccaa ctggcagccg ggatgaaacg cacgctcacg gcgtcgcagg
     1441 tgcagttcga gctgacgccg taccggccgt tgtcgagcgt cgaatgggag gtgctgaacg
     1501 aggcagccag ccgttacggg acgtttctcg gcaggcccgc aacgatcgtc cactccccct
     1561 gagccgtggg ctcgcgccgc tgccgatcca ggcatcgcga gccggcatca cagacccagc
     1621 gagtcgaagt actccttggc gacgtacttc gggaccacca tcgtccaggc gtcggtcacc
     1681 agttcggtca
//
LOCUS       DATBFZ010000493         1588 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_5450402, whole genome shotgun sequence.
ACCESSION   DATBFZ010000493 DATBFZ010000000
VERSION     DATBFZ010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5450402"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="VH419_15005"
     CDS             <1..216
                     /locus_tag="VH419_15005"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY7044976.1"
                     /translation="DAPVGPSGIQSTGHHFCVAVDDLEGAIAEFESRGIEYKRGRQGD
                     VVQIWIVDPAGNTIEFQQEIGQAEVRA"
     gene            213..1310
                     /locus_tag="VH419_15010"
     CDS             213..1310
                     /locus_tag="VH419_15010"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY7044977.1"
                     /translation="MTSLPLGRPARVGIAGLGRIYDLTIRAYRDNPDAEVVALCDRNT
                     ARLAERGAEWPDAKQYTDYDEFLGADIDVVEVLVPTPMHCEIVTAALHTGKHVNMQKP
                     FANTLDEADAMLAAAEASGTYLRVMENFIHYEPLRVLKEVLESGEIGDPAGLHMKMVA
                     TGVGGWDVPWSAWEWQFEQARAGRGILVFDDGWHKFSVAQWLFGPVTEVMAWIGNTEV
                     VPDLVVIDAPTTVMWTHANGVRGVFDITFAPDMLMKSDYYTNDERFEVTGRKGFVRVN
                     RCTSLGLQQPSVEVYHDGASRSYHALDDDWGSSFDRQTRDFLRYVVSGGDEPLWNGRQ
                     ARAVMAFIMAVYESAARTAPVTITAGGASNG"
     gene            1303..>1588
                     /locus_tag="VH419_15015"
     CDS             1303..>1588
                     /locus_tag="VH419_15015"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY7044978.1"
                     /translation="MAEITDIVIVGGGIGGASLAYALAREGLGVTVLEASTEYEDRVR
                     GESMQVWGVKEARDLGVEQVMVDAGAHIATVWRQYVEGVGAAGDIPAGMFI"
BASE COUNT          270 a          517 c          560 g          241 t
ORIGIN      
        1 gacgcgccgg tcggcccatc gggcatccag tcgaccgggc accacttctg tgtcgccgtc
       61 gacgacctcg aaggtgcgat cgccgagttc gaatcccgtg gcatcgagta caagcgtggc
      121 cggcagggtg acgtcgtgca gatctggatc gtcgacccgg cgggcaacac catcgagttc
      181 cagcaggaga tcggtcaggc ggaggtgcgc gcgtgacctc gctcccgctc ggccggccgg
      241 cgcgcgtcgg tatcgccggc ctcggccgca tctacgacct caccatccgc gcctatcgcg
      301 acaaccccga cgccgaagtg gtagcgctgt gcgaccgcaa tacggcacgg ttggccgagc
      361 gcggcgcgga gtggcccgac gcgaagcagt acaccgacta cgacgaattc ctcggcgccg
      421 acatcgacgt cgtcgaggtg ctggtgccga cgccaatgca ctgcgagatc gtgaccgcag
      481 cgctgcacac cggcaagcac gtcaacatgc agaaaccgtt cgcgaacacg ctcgacgaag
      541 ccgacgcgat gctcgcggcg gccgaggcat ccggcacgta cctgcgggtc atggagaact
      601 tcatccacta cgagccgctg cgcgtgctga aggaagtcct ggagtcgggc gagatcggtg
      661 acccggccgg gctccacatg aagatggtcg ccaccggggt cggcggctgg gacgtcccgt
      721 ggtcggcctg ggagtggcag ttcgaacagg cgcgggccgg ccgcggcatc ctcgtgttcg
      781 acgacggctg gcacaagttc tccgtggcgc agtggctgtt cgggccggtc acggaggtga
      841 tggcctggat cggcaacacc gaggtcgtgc cggacctggt cgtgatcgat gcgccgacca
      901 ccgtgatgtg gacgcacgcg aacggagtgc gcggagtctt cgacatcacc ttcgcgcccg
      961 acatgctcat gaagtccgac tactacacga acgacgagcg cttcgaggtc accggacgca
     1021 agggattcgt gcgcgtcaac cgctgcacat cgctcgggct gcagcaaccg tcggtcgagg
     1081 tgtaccacga cggcgcgtcc cgcagttacc acgcgctcga cgacgactgg ggttcttcgt
     1141 tcgaccgcca gacgcgcgac ttcctgcgct acgtcgtttc gggtggtgac gagccgttgt
     1201 ggaacggcag gcaggcccgc gccgtcatgg cgttcatcat ggcggtgtac gagtcggcgg
     1261 cgcgcaccgc gccggtcacg atcaccgcgg gaggcgcatc caatggctga gatcacggac
     1321 atcgtcatcg tcggcggcgg tatcggcggc gcgagcttgg cctacgcgct cgcacgcgaa
     1381 gggctcggcg tgacggtcct cgaggcatcc accgagtacg aagaccgcgt gcgcggcgag
     1441 agcatgcagg tgtggggcgt caaggaggcg cgcgacctcg gagtcgagca ggtgatggtc
     1501 gacgccggcg cgcacatcgc cacggtctgg cgccagtacg tcgagggcgt cggcgcggca
     1561 ggagacatcc cggccgggat gttcatcc
//
LOCUS       DATBFZ010000494         7052 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_3809001, whole genome shotgun sequence.
ACCESSION   DATBFZ010000494 DATBFZ010000000
VERSION     DATBFZ010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 7052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7052
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3809001"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="VH419_15020"
     CDS             complement(<1..194)
                     /locus_tag="VH419_15020"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020387941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEY7044979.1"
                     /translation="MTDANAVTYAAALAPDDRLALRGLRGVGHHGVFDHERRDGQEFV
                     VDVELGLDTRAAATADDLSAT"
     gene            complement(191..631)
                     /locus_tag="VH419_15025"
     CDS             complement(191..631)
                     /locus_tag="VH419_15025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012924013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY7044980.1"
                     /translation="MTTENDRIDEVLAANAAFYAAFERSDFDAMEAVWADDEGVVCVH
                     PGAAPIRGRSAVMRSWMALMAGAPYIQFFLTDVQASVVDDLASVTCTENVLSAGADTP
                     VGVFAGGSAAATNVLRYTSEGWRLWIHHASPVLSGTNARQEEAP"
     gene            complement(628..1512)
                     /gene="folP"
                     /locus_tag="VH419_15030"
     CDS             complement(628..1512)
                     /gene="folP"
                     /locus_tag="VH419_15030"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEY7044981.1"
                     /translation="MVYSAPPRYVAGLPRPDRTLVMGVVNVTPDSFSDGGMWLEPTAA
                     IDHGAALLDEGADIVDVGGESTRPGAERPSVEEELSRVIPVVRALAGRGALVSIDTMR
                     SEVARRSVDAGAVAVNDVSGGRAEPEMLSAVAELGVPFVCMHWRGHSTTMQEQASYDD
                     VVSDVIDELGERLKAADEAGIAPERLAVDPGLGFAKTGEHNWAVLRRLEELHQLGRPV
                     LVGSSRKAFLGALLADETGRPRPATERDDASAALSALAALAGAWCVRVHDVRRSLDAV
                     KVAARWARESTPALGADR"
     gene            complement(1566..2171)
                     /gene="folE"
                     /locus_tag="VH419_15035"
     CDS             complement(1566..2171)
                     /gene="folE"
                     /locus_tag="VH419_15035"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011753938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEY7044982.1"
                     /translation="MVDQGLDVSVTDGVRPFDHAKVEAAVREILVAIGEDPERDGLRH
                     TPARVARAYAETFAGLRMNPDHVLTTTFDIGHDELVLVKDIEVWSTCEHHLVPFNGVA
                     HVGYIPNAEGRITGLSKLARLVDVYARRPQVQERLTTQVADSVMKFLQPRGVIAVFEC
                     EHLCMTMRGVRKPGSKTVTSAVRGQLRDPATRAEAMSLILG"
     gene            complement(2182..4233)
                     /gene="ftsH"
                     /locus_tag="VH419_15040"
     CDS             complement(2182..4233)
                     /gene="ftsH"
                     /locus_tag="VH419_15040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004810424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEY7044983.1"
                     /translation="MKRWFRGPWLWVVLFAIVILVVIDVVSNNDGSKQVPTSEMVATI
                     QKGGVKEVTLVDGDQKIEATLDNGDKISSSWAAAQGPALTRTIQKAVDEGKIDQSYNV
                     EVPKPSLIWSFLGTIFPILLIVLFFLFMMNSLQGGGGRVMQFAKSRAKVVSKDTPKTT
                     FADVAGVDEAIEELHEIKEFLQQPAKFQAVGAKIPKGVLLYGPPGTGKTLLARAVAGE
                     ANVPFYSISGSDFVEMFVGVGASRVRDLFEQAKENAPAIVFIDEIDAVGRHRGAGMGG
                     GHDEREQTLNQLLVEMDGFDVRGGVILIAATNRPDVLDPALLRPGRFDRMVGVSAPNM
                     DGRHKILKVHSRGKPIASGVDLLAVARRTPGFTGADLANVLNEAALLTARQDKKLIDG
                     YLLDEAIDRVSMGPQRHSHIMSDAEKLMTAYHEGGHALVAAALPGTDPVTKITILPRG
                     RALGFTQVLPEADKSSVSRNELLNQLAYALGGLAAEQLIFHDPTTGSSNDIEKATKVA
                     RAMVTSYGMSERLGAVKLGEENSEPFLGRDFGHTRNYSEEIAAIIDEEVNSLLTKAHQ
                     EAFDILVDNRDVLDNLVTELIEKETIDRNGLAEIFADLRMRPRRPAWTGSESRIPSSV
                     PPVEVPASSNGTRPEPKPEITVGPGAESPGPGFGEGPPSGPPGAPPPGPPLGPSVGSQ
                     D"
     gene            complement(4506..5057)
                     /gene="hpt"
                     /locus_tag="VH419_15045"
     CDS             complement(4506..5057)
                     /gene="hpt"
                     /locus_tag="VH419_15045"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020387935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEY7044984.1"
                     /translation="MDVSNVEKDLSRILITEDEIQQRLVELARDIEADYAGTDLLLVG
                     VLKGACMVMADLARSLNRHVAMDWMAISSYGSATTSSGVVRILKDLDTDISGKHVLIA
                     EDIIDTGLTLSWLVANLESRGPESVNICTLLRKPDALQMAVEVKYVGFDIPNEFVVGY
                     GLDYAERYRNLRSVATLAPHVYT"
     gene            complement(5109..6092)
                     /gene="tilS"
                     /locus_tag="VH419_15050"
     CDS             complement(5109..6092)
                     /gene="tilS"
                     /locus_tag="VH419_15050"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011753934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEY7044985.1"
                     /translation="MTAGLDPAVAAVRHAVRDALADVEPGAQVLVACSGGADSMALAD
                     AAVFEGRHAGWLVGAVTVDHRLQPGSDKVAEDVAVRLASLGCDPVRVAVTEVVAHGGP
                     EAAARTARYAELERVAVEYDATVLLGHTLDDQAETVLLGLARGSGTRSLSGMAPVTGR
                     FRRPLLGIRREQTRRACQAAGLPTWDDPHNDDETFSRVRVRRRLLPALEATLGPGMVE
                     ALARTASAARRDADLLDDLAADVFATASGADGLDADVLAGAPSALRTRVLRVAALQAG
                     CPATDLFAVHVDEMERLVTDWHGQGPLTLPGGVRAARGRGKITFTTAAVGR"
     gene            complement(6133..>7052)
                     /locus_tag="VH419_15055"
     CDS             complement(6133..>7052)
                     /locus_tag="VH419_15055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012924020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HEY7044986.1"
                     /translation="GLYAESASAPVLVVDRGGWVQANADGFKQVLRPLIAKMREKRGE
                     PGAVTAAIGSRVTGLEAGGLLAYLSNKVLGQFDPFYSGPAGPSGTTYGGRLLLVAPNI
                     VHVERELEATPHDFRLWVCLHEETHRVQFTAVPWLRQHLLSELELLLQSTELDPAKLG
                     SMMRDGIEAVGRLVRGDENVSLLDIMQTPEQKAVVERLTAVMSLLEGHADVVMDGVGP
                     DVVPSVESIRKKFTKRRAGAGPIDQMIRRLLGFDAKLRQYRDGAHFVRGAIEKVGMDG
                     FNAVWSEPANLPTRDEIGDPAQWVTRVHG"
BASE COUNT         1075 a         2491 c         2356 g         1130 t
ORIGIN      
        1 gttgcggaca ggtcgtcggc ggtcgccgca gcccgggtgt ccaggccgag ctcgacgtcg
       61 acgacgaact cctgcccgtc gcgccgctcg tggtcgaaga cgccgtggtg gccgacgccg
      121 cgcagcccgc gcagcgccag ccggtcgtcc ggcgcgagcg ctgcggcgta cgtcaccgcg
      181 ttcgcatcag tcacggagcc tcttcctgtc tcgcgttcgt gcccgacagt accggggaag
      241 catggtggat ccacaaccgc cagccctccg acgtgtaccg cagcacgttg gtcgccgccg
      301 cgctcccgcc ggcgaagacg ccgacgggtg tgtcggcgcc ggccgacagc acgttctccg
      361 tgcaggtgac ggatgccagg tcgtcgacca ccgatgcctg gacgtcggtg aggaagaact
      421 ggatgtacgg cgctcccgcc atcagcgcca tccacgaccg catcaccgcg ctccggccac
      481 gaatcggcgc cgcccccgga tgcacacaca cgacaccctc gtcgtcggcc cacaccgcct
      541 ccatggcgtc gaagtccgaa cgctcgaagg ccgcgtagaa cgcggcgttg gcggccagga
      601 cctcgtcgat gcggtcgttc tccgtcgtca ccggtcggcc cccagcgcag gggtgctctc
      661 acgagcccag cgcgccgcga ccttgaccgc gtcgagcgaa cgacgtacgt cgtgcacgcg
      721 cacacaccat gcgccggcga gggctgccaa ggccgacagg gccgcggacg cgtcatcccg
      781 ctcggtcgcc gggcgaggac gacctgtctc gtcggccagc agcgcgccca ggaatgcctt
      841 gcggctcgac ccgacgagca ccggccggcc cagctggtgc agctcctcca gtcgccgcag
      901 cacggcccag ttgtgctcgc cggtcttcgc gaagccgagc ccggggtcga ccgccaaccg
      961 ctccggcgcg atgcctgctt cgtcggctgc cttcagccgc tccccgagct cgtcgatcac
     1021 gtccgacacg acgtcgtcgt acgacgcctg ctcctgcatc gtcgtcgagt ggccacgcca
     1081 gtgcatgcac acgaacggga cgcccagctc ggccacggcc gacagcatct cgggttcggc
     1141 acgtccaccg gagacgtcgt tcactgcgac cgctccggca tccacggagc gccgtgcgac
     1201 ctcgcttcgc atcgtgtcga tactgacgag agcaccccgc ccggcgagcg cgcggacgac
     1261 cgggatcacc cgcgacagct cctcctcgac gctgggccgc tctgcgcccg gtcgggtcga
     1321 ctcaccgccg acgtcgacga tgtcggcgcc ttcgtcgagc agggcggcac catggtcgat
     1381 ggcagcggtg ggctccagcc acattccccc gtcggagaac gagtccggcg tgacgttgac
     1441 gacacccatc accaacgtgc ggtccggacg cggtagaccg gccacgtagc gagggggagc
     1501 gctgtagacc atggtggtcc gccaacctag ccgaccggtg ttcggtggct ccagggcgtg
     1561 gcctatcagc cgaggatgag gctcatggcc tcggcgcggg tcgccgggtc acggagctga
     1621 ccgcggacgg cgctcgtcac ggtcttcgac ccgggctttc ggacgccacg catcgtcatg
     1681 cagagatgct cgcactcgaa caccgcgatc acgccgcgcg gctggaggaa cttcatcacg
     1741 gagtccgcaa cctgggtcgt cagccgctcc tggacctgcg gtcgacgggc gtagacgtcg
     1801 accagccggg ccagcttcga cagaccggtg atgcgtcctt cggcattcgg gatgtacccc
     1861 acgtgcgcca ccccgttgaa cggcaccaga tggtgctcgc acgtcgacca gacctcgatg
     1921 tccttgacga gcaccagctc gtcatgcccg atgtcgaacg tcgtcgtgag cacgtggtca
     1981 gggttcatcc gcagacctgc gaacgtctcg gcgtacgcac gcgccacccg ggccggggtg
     2041 tggcgcaggc cgtcccgctc cgggtcctcg ccgatggcga ccaggatctc gcggacggcc
     2101 gcctcgacct tcgcatggtc gaagggacgc acaccgtcgg tcacactgac gtcgaggccc
     2161 tgatccacca tcgccgccga cctagtcctg cgagcccacg gacggaccga gtggcgggcc
     2221 gggcggcggc gcacccggag gtccgctcgg cggcccctca ccgaagccgg ggcccggcga
     2281 ctccgctccc gggccgacag tgatctccgg tttgggctcc ggccgtgtcc cgttgctgct
     2341 cgccggtact tcgacgggtg gaaccgacga ggggatccgg ctctcggatc cggtccaagc
     2401 cggtcgccgt ggccgcatcc ggaggtcggc gaagatctcg gccaggccgt tgcgatcgat
     2461 cgtctccttc tcgatcagct cggtgacgag gttgtcgagc acgtcccggt tgtcgacgag
     2521 gatgtcgaac gcctcctggt gggccttcgt caggagcgag ttgacctcct cgtcgatgat
     2581 cgccgcgatc tcctcggagt agttgcgggt gtggccgaag tcgcggccga ggaacggctc
     2641 gctgttctcc tcgcccagct tcacggcacc gagacgctcg ctcatgccgt agctggtgac
     2701 catggcacgg gccaccttcg tggccttctc gatgtcgttg gacgacccgg tcgtcgggtc
     2761 gtggaagatc agctgctcgg cagcgaggcc accgagggcg taggcgagct ggttgagcag
     2821 ctcgttgcgc gacaccgacg acttgtcggc ctcggggagc acctgggtga agccgagtgc
     2881 gcggccgcgc ggcaggatcg tgatcttcgt gaccgggtcg gtgcccggga gcgcggctgc
     2941 gacgagggcg tggcccccct cgtggtacgc ggtcatgagc ttctcggcgt cgctcatgat
     3001 gtgcgagtgg cgctgcggac ccatgctgac ccggtcgatc gcctcgtcga gcaggtagcc
     3061 gtcgatgagc ttcttgtcct gccgggcggt gagaagtgcg gcctcgttca gcacgttggc
     3121 gagatcggcg cccgtgaagc ccggcgtacg acgcgccact gccaacaggt cgacgccact
     3181 cgcgatcggc ttgccgcggg agtgcacctt gaggatcttg tgacggccgt ccatgttcgg
     3241 ggctgagacg cccaccatcc ggtcgaaccg cccgggtcgc agcagcgcgg ggtcgagcac
     3301 gtcgggacgg ttcgtggccg cgatgaggat cacgccacca cgcacgtcga agccgtccat
     3361 ctcgaccaac agctggttga gggtctgctc gcgctcgtcg tgcccgccgc ccataccggc
     3421 accgcggtgg cgaccgacgg cgtcgatctc gtcgatgaag acgatcgccg gggcgttctc
     3481 cttcgcctgc tcgaagaggt cgcgaacccg tgaggcaccg acaccgacga acatctcgac
     3541 gaagtcggat ccggagatgg agtagaacgg cacgttcgcc tcgccggcca cagctcgggc
     3601 gagcaacgtc ttcccggtgc cgggtggccc gtagagcagc acgcccttgg ggatcttcgc
     3661 gcccaccgcc tggaacttcg ccggctgctg caggaactcc ttgatctcgt gcagctcctc
     3721 gatcgcctcg tcgaccccgg cgacgtcggc gaacgtggtc ttcggtgtgt ccttgctgac
     3781 caccttcgcg cgcgacttgg cgaactgcat cacacgtcca ccaccgccct gcaggctgtt
     3841 catcatgaag aggaagaaca gcacgatcag caggatcggg aagatcgtgc cgaggaagct
     3901 ccagatcagg ctgggcttgg gaacctcgac gttgtaggac tggtcgatct tgccctcgtc
     3961 gaccgccttc tggatcgtcc tcgtcagggc cggcccctgg gcggctgccc agctggagga
     4021 gatcttgtcg ccgttgtcga gcgtggcttc gatcttctgg tcaccgtcga ccagtgtgac
     4081 ctctttcaca ccacccttct ggatggtggc gaccatctcg gaggtcggga cctgtttcga
     4141 gccgtcgttg ttggacacca catcgatgac gacgaggatc acgatggcaa agaggacgac
     4201 ccagagccag ggtccacgga accaacgctt cacgcttgtg taccggagca gacggccccg
     4261 ttcctcctac caactcactc gatcgacgct gcttcaaggg ggtgcgcgcg gggtttcgcg
     4321 gccacgcgct accttcaagg gtccatccat tgtcgcctac ggaccaaatc ggcgtacatg
     4381 acggcgcaaa actggtctcc cgctccaacc ctggtcccag cgaaatgcgg gaaacatcat
     4441 gctcgcaacc gcgtgtcgcc gtgacctttc ccgcaaatcg cggaagccag gcagccggcg
     4501 aggggtcagg tgtacacgtg gggggcgagt gtggcgaccg agcggaggtt ccggtagcgc
     4561 tccgcgtagt cgagcccgta gccgacgacg aactcgttcg ggatgtcgaa gccgacgtac
     4621 ttcacctcaa ccgccatctg caacgcgtcg ggcttgcgca gcaacgtgca gatgttcacc
     4681 gactccggac cgcgtgactc aaggttcgcg accagccacg acagtgtcag cccggtgtcg
     4741 atgatgtcct cggcgatgag cacgtgcttg cctgagatgt cggtgtcgag gtccttgagg
     4801 atgcggacga cgccggagct cgtcgtggcc gacccgtacg acgagatcgc catccagtcc
     4861 atcgccacgt gccggttgag cgaccgggcg aggtcggcca tgaccatgca cgcacccttg
     4921 agcacaccga ccagcagcag gtccgtaccg gcgtagtcgg cctcgatgtc acgcgccagc
     4981 tcgacgagcc gctgctggat ctcgtcctcg gtgatcagga tgcggctcag gtccttctcg
     5041 acgttgctga cgtccactgc cgtctcctgc gcttccgcgt gatgtcgtgg gcctgcggct
     5101 gggagccctt agcgtcccac agcagcggtg gtgaacgtga tcttgccccg cccgcgagcc
     5161 gctcggactc cgccgggcag ggtgagcggt ccctgtccgt gccagtccgt gacgaggcgt
     5221 tccatctcgt ccacgtgcac cgcgaacagg tcggtagcag ggcagcccgc ctggagtgca
     5281 gccaccctga ggacgcgggt gcgcagggca ctgggagcgc cggccagcac atcggcgtcc
     5341 agaccgtcgg cgccggatgc ggtggcgaac acgtcggcag ccaggtcgtc gagcaggtcg
     5401 gcgtcgcgcc gggcagccga cgcggtccgg gccagcgcct cgaccatgcc gggcccgagc
     5461 gtggcttcga gtgccggtag caaccgccga cgcacgcgga cgcgcgaaaa cgtctcgtcg
     5521 tcgttgtgcg ggtcatccca cgtcggcaga ccagcggcct gacaggcccg gcgcgtctgc
     5581 tctcgacgta tccccaggag cggacggcgg aagcgccccg tcaccggagc catcccgctc
     5641 agtgaacgcg ttccgctccc gcgagccaag cccagcagca ccgtctcggc ctggtcgtcg
     5701 agcgtgtgcc cgagcagcac cgtcgcgtcg tactcgacgg cgacacgttc gagctcggcg
     5761 taccgggctg tcctggccgc cgcctccggg cctccgtggg ccacgacctc ggtgaccgcg
     5821 acgcggacgg ggtcgcagcc gagcgacgcc agcctcaccg cgacatcctc ggcgaccttg
     5881 tccgagccgg gctgcagccg gtggtcgaca gtgaccgccc cgacgagcca gccggcgtgc
     5941 cgtccttcga acacggcggc gtcggcgagc gccatcgagt cggctccacc cgaacaggcc
     6001 accaatacct gtgcgccggg ctcgacgtcg gccagtgcgt cacgaacggc atggcgtacc
     6061 gccgcgacgg cgggatcgag accggccgtc acgttgccct cgtggccctt tcgccgtgat
     6121 tccggcgcgg gctcagccgt ggacgcgggt gacccactgc gccgggtcgc cgatctcgtc
     6181 gcgggtgggc aggttcgccg gctccgacca gaccgcgttg aagccgtcca tgccgacctt
     6241 ctcgatcgcg ccgcggacga agtgcgcgcc gtcacggtac tgccggagct tggcgtcgaa
     6301 tccgagcagc cggcggatca tctggtcgat gggcccggcg ccggcccgtc gcttcgtgaa
     6361 cttcttccgg atgctctcca cgctggggac gacgtctggt ccgacgccgt ccatcacgac
     6421 gtcggcatgc ccttccagca gcgacatcac ggcggtgagc cgttcgacga cggccttctg
     6481 ctcgggtgtc tgcatgatgt cgagcagcga cacgttctcg tcaccccgga cgagacggcc
     6541 gacggcctcg atcccgtcgc gcatcatcga gccgagtttc gccggatcga gctcggtgct
     6601 ctgcagcagg agctccagct cggagagcag gtgctgccgg agccagggga ccgcagtgaa
     6661 ctgcacgcgg tgggtctcct cgtgcaggca cacccacagc cggaagtcgt gcggcgtcgc
     6721 ctcgagctcg cgctcgacgt gcacgatgtt cggcgcgacg agcagcagcc ggccgccgta
     6781 cgtcgtaccg ctcggtccgg ccggcccgct gtagaagggg tcgaactggc cgagcacctt
     6841 gttcgacagg taggcgagca gtccgccggc ctccagcccc gtcacccgag acccgatcgc
     6901 ggcggtcacc gcaccgggtt cacctcgctt ctcgcgcatc ttggcgatga gcggacgcag
     6961 cacctgcttg aacccgtcgg cgttggcctg cacccagcca ccacggtcga ccacgagcac
     7021 cggcgcgctg gccgactcgg cgtacaaacc gg
//
LOCUS       DATBFZ010000495         1818 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_5131973, whole genome shotgun sequence.
ACCESSION   DATBFZ010000495 DATBFZ010000000
VERSION     DATBFZ010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5131973"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="VH419_15060"
     CDS             complement(<1..228)
                     /locus_tag="VH419_15060"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY7044987.1"
                     /translation="MVVPKLLTDAGVSEREAEVLGLVGDHLTNAEIARRLFISVRTVE
                     SHVSSLLRKLDQADRRGLAVLAASLAAQARPA"
     gene            442..1005
                     /locus_tag="VH419_15065"
     CDS             442..1005
                     /locus_tag="VH419_15065"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEY7044988.1"
                     /translation="MSTTTESPMRNGVDTATLFATLDAVKADPDIAKFKFRVNNRWVN
                     GTHNRSTIDGFYGARQELSHRQSSTFDADHPEVLVGADNAPTPAEHLLHALASCLTSG
                     LANIAAARGVNLTSVESTVEGDIDLLGILGLSDEVRNGYQQIRVSFRLEGDEPDKLRS
                     VVEQARLRSAVYDVITNGVPVSIDVTA"
     gene            1010..>1818
                     /locus_tag="VH419_15070"
     CDS             1010..>1818
                     /locus_tag="VH419_15070"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY7044989.1"
                     /translation="MPFLRKSEFTMARRTSTSATYDVVVVGARPAGAATALLLSRAGL
                     RVLVVDRDRHGADTVSTHALLRGGVLQLARWGVLGRVEAAGTPPVRGCVFHFGADQIE
                     IPVKPAAGVPALYAPRRTVLDAILVDAAKDAGAEVRFGIAVTDLRRDRQGRVTGVAGR
                     DERGTAFEARARIVVGADGIGSIIARRSGAPVERKGNSACAFIYGYWDGLPIVDYELC
                     YQPGIAAGFFPTNDRQTCVFVGTTPQRYRRETKAAGVAVTYARLLDEAAPGA"
BASE COUNT          314 a          652 c          589 g          263 t
ORIGIN      
        1 ggccggtcga gcctgcgccg cgagcgacgc ggccagcaca gcaagcccgc gccggtcggc
       61 ttggtcgagc ttgcgcagca gcgacgagac gtggctctca acggtgcgca ccgagatgaa
      121 cagacgccgc gcgatctcgg cgttggtcaa gtggtcaccc accagcccca gcacctcggc
      181 ctcccgctcg gagaccccgg cgtcggtcaa cagcttcggc acaaccatcg gactgatcgg
      241 acaagcatcc catgcccaac acctcccgcg aactgcggga agttccggga ttccgcggcc
      301 ccagaacgcg gaacctcccg caattcgcgg aagatccgtg gtcgatccgt gaccagcacg
      361 gatgccttgt cgctgacccg agcggacagt gaccacatgc cagcaagctc cgcaatgccg
      421 accaccaagg aggtccaacc gatgagcacc accaccgagt cacccatgcg gaacggcgtg
      481 gacaccgcca cgttgttcgc aaccctggat gccgtgaagg ccgacccgga catcgccaag
      541 ttcaagttcc gggtcaacaa ccgctgggtc aacggcacac acaaccgctc gacgatcgac
      601 ggtttctacg gcgcccgaca ggagctcagc catcggcagt cctccacctt cgacgccgat
      661 caccctgagg tgctggtcgg cgcagacaac gcgccgacgc cggctgaaca cctgctgcac
      721 gcactggcct cctgcctcac ctccggactg gccaacatcg ccgcggcgag aggcgtcaac
      781 ctgacctcgg tcgagtcgac ggtcgaggga gacatcgacc tgctcggcat cctcggcctc
      841 tccgacgagg tccgcaacgg ctaccagcag atccgggtca gcttccgcct cgaaggcgac
      901 gagccggaca agcttcgcag cgtggtcgag caggcacggc tgcgctcagc cgtgtacgac
      961 gtcatcacga acggcgtgcc ggtcagcatc gacgtgaccg cctgacccga tgcctttcct
     1021 ccgaaagagt gagttcacga tggcgcgtcg tacgtcgacg tccgcgacgt acgacgtcgt
     1081 tgtcgtcggg gcgcggccgg ccggggcagc caccgcactc ctgctgtccc gggccgggct
     1141 gcgggtgttg gtggtcgacc gggaccgcca cggcgcggac accgtgtcga cccacgccct
     1201 gctgcgcggt ggtgtgctgc aactggcacg ctggggcgtt ctcggtcggg tcgaagccgc
     1261 cggcaccccg ccggtgcgtg gctgcgtgtt ccacttcggc gccgaccaga tcgagatacc
     1321 ggtcaaaccc gctgccggcg ttcccgcctt gtacgccccg cgccgcaccg tgctggacgc
     1381 gatcctggtc gacgcggcca aggatgccgg cgccgaggtt cgcttcggca tcgccgtcac
     1441 cgacctgcgc cgggaccggc aaggcagggt gaccggggtc gcgggacggg acgaacgggg
     1501 tacggcgttc gaggcccgcg ccaggatcgt cgtcggcgca gacgggatcg gttcgatcat
     1561 cgcccgacgc tccggagcac cggtggaacg caaagggaac agcgcctgcg cgttcatcta
     1621 cggatactgg gacggcctgc cgatcgtgga ctacgagctg tgctatcagc ccggaatcgc
     1681 cgcagggttc ttccccacca atgaccgcca gacgtgtgtc ttcgtcggca ccaccccgca
     1741 gcgttaccga cgtgagacca aggcggccgg cgtcgcggtg acctatgccc ggctcctgga
     1801 cgaggccgca ccgggtgc
//
LOCUS       DATBFZ010000496        22208 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_3812537, whole genome shotgun sequence.
ACCESSION   DATBFZ010000496 DATBFZ010000000
VERSION     DATBFZ010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 22208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22208
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3812537"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..510)
                     /locus_tag="VH419_15075"
     CDS             complement(<1..510)
                     /locus_tag="VH419_15075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008360570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5931 domain-containing protein"
                     /protein_id="HEY7044990.1"
                     /translation="MPRVNSWSSPAAIAVENRLFSALVVLRVVLLVNAVALNIYRADT
                     FQHSAAGAACVVVMVLWTGFATWAYAEPRRRTFPLLATDLALAVALLLVTPLVKGAEF
                     SASVPGFWVIGALIAWSIHYRWVGGLVAGLLLAAADLSQRQEIGQSDYGNAFLLIIGG
                     PTVGFMCESL"
     gene            691..1122
                     /locus_tag="VH419_15080"
     CDS             691..1122
                     /locus_tag="VH419_15080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7044991.1"
                     /translation="MTGASESPGGSAPVPWTPAPYSRNGDRHVPDSGWRRWTPRILVT
                     LLIVGGTAALSALGLAFLYGMNPMGDEWVCGDGEAPTTTYCYPTDEPLPAGVQWDPLG
                     NRPMPYNCDKNGWTLIEHNRGDKQDCLNNNLPMPVGWHEVK"
     gene            complement(1164..1757)
                     /locus_tag="VH419_15085"
     CDS             complement(1164..1757)
                     /locus_tag="VH419_15085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020578820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03086 family metal-binding protein"
                     /protein_id="HEY7044992.1"
                     /translation="MAPSELVTAAPARRHRLIADDFARRVHGTKDWSAAAPVSGWTAR
                     DVVVHLVGWLPGFVAAGSDVRLPEVPSPAEQPAPAWDAHQRAVQGLLDDPATAGVEFA
                     NRHTGSMPLAQAIDRFYTSDVFMHTWDLARATSQDDALDPDLCDVLLSGMEPIEDLMR
                     DSGQFGPRVPVPAIADPQTRLIGFTGRDPKWRAATMR"
     gene            complement(1744..2244)
                     /locus_tag="VH419_15090"
     CDS             complement(1744..2244)
                     /locus_tag="VH419_15090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007079532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEY7044993.1"
                     /translation="MSTASTDTRYDQAVIEADPTIPAIHIWRDFAGTPDQLFRAHTDP
                     ELYARWIGPADRTAVIDRWDATDGGSFRFLDSRDDDQQAFRGCFHTVRPDRIVQTFTW
                     EGMPDDVALETMTFEDLGDGRTRLHAVSLCDSFEGRDAWLRSGMEVGVNDGYAEIDRM
                     LADGSL"
     gene            complement(2241..2597)
                     /locus_tag="VH419_15095"
     CDS             complement(2241..2597)
                     /locus_tag="VH419_15095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007079531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY7044994.1"
                     /translation="MPDQLSRVFAALADPTRRDMVARLTDGDATVGTLAAPYAVSLQA
                     VSKHLKVLEEAGLVTRSREAQRRPVHLEAEVFDLMTRWIERYRRRVEERYQRLDAVLA
                     ELDEEQTETPTEGTAS"
     gene            complement(2713..4101)
                     /locus_tag="VH419_15100"
     CDS             complement(2713..4101)
                     /locus_tag="VH419_15100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007079312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dTDP-4-dehydrorhamnose
                     3,5-epimerase family protein/NAD(P)-dependent
                     oxidoreductase"
                     /protein_id="HEY7044995.1"
                     /translation="MSDITVEPTPIPGLLVVRVPLHEDARGWFKEAWQREKMTALGLP
                     DFGPVQANMSYNANRGTTRGIHAEPWDKFVTVAAGRVFGAWVDLRTGPTFGTVFTTEV
                     DPSVCVYVPRGVGNSYQALAAATTYCYLVNDHWRPGQEYPALALDDPTAAIAWPIPLA
                     EADVSEKDRTRNPRLAEVVPLQPRRTLVTGSNGQLGRALAAEFPDATTAGSHELDLTD
                     EAAVAAWPWHEYGFVLNAAAYTAVDAAETPEGRRSAWRANAGIPTTLARIATEHRLTL
                     VHYSTDYVFDGTQVDGGHREDEALSPLGVYGQSKAAGDLVVGTCPRHYLVRTSWVVGD
                     GNNFVRTMRRLAADGVSPSVVDDQVGRLTFTSELARATRHLLEVDAAPGTYNVTNAGP
                     PQSWADVARRVFELSGRDPEDIRPVSTEEYDAGRSSAPRPRCSLLSLDKIAATGFVSE
                     PVDDALIAYLTD"
     gene            complement(4104..5102)
                     /gene="rfbB"
                     /locus_tag="VH419_15105"
     CDS             complement(4104..5102)
                     /gene="rfbB"
                     /locus_tag="VH419_15105"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016666789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEY7044996.1"
                     /translation="MNRILVTGGAGFIGSNFVHHLMRNTDAIVTVLDKLTYAASPEAF
                     EGLPEDRFTLVKGDVADAALVEPLVASHDTVVHFAAESHNDNSLRDPTPFVHTNLVGT
                     FTLLEAVRKADVRLHHVSTDEVYGDLALDDPKRFTEDTPYRPSSPYSASKAGSDHLVS
                     AWVRSFGIRATISNCSNNYGPWQHVEKFIPRQITNVLAGGRPRLYGSGENVRDWIHTE
                     DHSSAVLTILERGRLGETYLIGADGERSNIAVVRLILRLMGRDEDDFDHVTDRVGHDL
                     RYAIDSGKLRSELGWTPRFGDFEAGLAQTIDWYRDNEAWWWPHKDAIEAAYAAKGQ"
     gene            5161..6036
                     /gene="rfbA"
                     /locus_tag="VH419_15110"
     CDS             5161..6036
                     /gene="rfbA"
                     /locus_tag="VH419_15110"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007079314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEY7044997.1"
                     /translation="MRGIILAGGTGSRLHPITRAISKQLMPVYDKPMIYYPLSTLMLA
                     GIREVLVITTPHESEMFRRLLRDGSQFGIAIEYAVQPSPDGLAQAFLIGEDHLAGGPA
                     GLVLGDNIFYGSGLGSRLRRFAEIDGAAVFGYRVADPSAYGVVEVGADGRALSLEEKP
                     QKPRSSLAIPGLYFYGPDVVERARTLKPSARGELEITDLNRLYLEEGRLRVEILPRGS
                     AWLDTGTFDDLNDASTYIRALEHRQGTKVGSPEEVAWRMGYIDDDQLGELAQDMLKSG
                     YGRYLLDLLAEERGS"
     gene            6148..6912
                     /locus_tag="VH419_15115"
     CDS             6148..6912
                     /locus_tag="VH419_15115"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY7044998.1"
                     /translation="MRKHQLLRVIHERLRPRTYFEIGVRKGVSLGLSRVPSVGVDPFY
                     MVTDELRCDVHLVRTTSDEFFARKHPFAHFKQQVIDLAFIDGMHLAEFALRDFINTER
                     HCHPASVIVIDDVLPRTVEEGGRGRSGSADGGAWAGDVYKIIDVLRRNRPDLVVLEMD
                     TQPTGTLVILLPDPTNRALAAAYDEAVPAMVVPDPQDVPAHILRRTNARDPEKLIAAP
                     IWDALREARRLSRTEAGAQVQEALAAAGLTDEAVLR"
     gene            6909..9203
                     /locus_tag="VH419_15120"
     CDS             6909..9203
                     /locus_tag="VH419_15120"
                     /inference="COORDINATES: protein motif:HMM:NF016464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 61 family protein"
                     /protein_id="HEY7044999.1"
                     /translation="MSRNTMPVDELLSWARREGPAIQVGLRLPGADLAPGKTTVRLTR
                     RKGELQVDADATGESDAVTLAFTVPRTTLAPPAWRIAVQTGADGRFQPVEARLLAPKD
                     QPVAVLPGPPPDTKMPAPSPRRPGRAMRTPVATPTRRVLRRGRSLARAARRAARKLTA
                     GRGSDRSADRPGRRPGTQPDRPALRLDGPALRAVRAAGKHPVVVLADASTEHAVSAWL
                     PALRGTDVTMFRIGADSPRAATAADVVDVADTADVADAADAADVVDANDVDDVERRLF
                     PTEAPAALVVALSPATLRSLATDHVQLLVRLFPHLRHGGAYVAYAVPTGGPSASARDH
                     AIATFRRALEESAGDLTAGSPGGRKPRMRGIRDVVVRPGFVLLRKNRRDALHVREEQV
                     ADLLPAREPGTEVSILEVRPSGSLQVTATEISYGPRQAEPWPKRLDYPELTLRHYRGD
                     LMSMGSMLLTAESTILPESFRWPHRSKLGQASVRSAGPHFSTVPRVKGAPILEGDYYF
                     LDCLFSGHFGHLLTEVVCRLWGWEAARRELPNVKALFHTNPRRGRHGALERKLFAAYG
                     VPEADLVSTDRPVRLRSVVGASPMWHNAPPFYVHPDIQETWARLTSGLLDGRPPAEHE
                     RIFVSRGDDLNRRRCRNQPEVERFFADRGFHVFYPERLPLEEQVALFAGARVVAGFGG
                     SAMFNMLHCRRLEKTIVISHNSYEARNENLFMSVLGGELHYFWQESDVSLTGGATNRQ
                     KRRSSYAFDFENYGTDLARVISQP"
     gene            9260..9646
                     /locus_tag="VH419_15125"
     CDS             9260..9646
                     /locus_tag="VH419_15125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013861932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY7045000.1"
                     /translation="MITSVHSLIYSDDAPATRAFFRDVLGWPYVEDTESDAGWLIFQT
                     GPSELGVHPTSGMHEGRPWSATRHHAISLMCDDIAATRKELEDKGATFSGEIADHGYG
                     QVTMLQVPGADDIQLYEPSHPTAYDL"
     gene            complement(9661..9831)
                     /locus_tag="VH419_15130"
     CDS             complement(9661..9831)
                     /locus_tag="VH419_15130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7045001.1"
                     /translation="MKDDVVLQDVQLLEEIELLGSLMVVASSADRQLTQREIDHVLGV
                     GGSQTPTLEISG"
     gene            10244..12685
                     /locus_tag="VH419_15135"
     CDS             10244..12685
                     /locus_tag="VH419_15135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7045002.1"
                     /translation="MRTHAYRTPRRTAGWTVRRLPAIAALILAAAFLPACTSSSDHDA
                     TNKTNNQSGESEQSEDEHAEAEGEEPGEEEGEEGGEGWNEDIEHAAEAAGPELPEDSF
                     LFQRLMTSGSPDLNAFGRAKQQATALTALAARTTPQLKQRRWEFLGPENVGGRVTDGV
                     VDPTRPNTIYVAASTNGVWRSSDAGQTFTSVWPDDITHSMGALAIAPNGMLYAGTGET
                     NPGGGSITYGGNGVYRSTNHGLSWQRVGLTRSGTIGRIVIDPKAPDRIWVAVSGNLFT
                     PGGQRGVYESTDGGDTWKRSLRPPNELTGATDIAIDPANSRHLIATMWDHIRRPSARI
                     YTGIGSGVWETHNAGRTWKRLGPAQGLLAPSGNTGRIGVTFSPADADRAWIIYSNDET
                     GAFQDFFRSDDNGASWQRPPGTDQLADSQSVYGWWFGRLYADPEDADRLYVLGLDLWE
                     TRDAGATFDTVPDIHVDQHVVMYDPAVPDRTYVGNDGGLYVSEDNGASFTKSEDQPWS
                     QYSGVDVSEQDQSHIVGGLQDNGTQATWVADGSDWGSIFGGDGQRSLIDPTDADNYYA
                     CAQYGVCAGFEDGSSFQMQFTSKRFPYFMQYAFNPKNTDVMYGGGSELNKSTDEGHTW
                     TVISDDLGKGEAGDEPNPLYRNHYGTVSALAVAPSRPRIVWAGTDNGYLYRSADAGAS
                     WQELPNPVRPKLWIQRLVVAPDNARTVYLTFSGYREGDNSAYVLRTRNNGQTWTDITA
                     NLPHAPVNDLAVVGDRLYVATDVGVFTSPASRPRWTAYGSGMPQLVTTDLRYVPENAT
                     LYVATFGMGVWSIKA"
     gene            12912..15014
                     /gene="glgX"
                     /locus_tag="VH419_15140"
     CDS             12912..15014
                     /gene="glgX"
                     /locus_tag="VH419_15140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HEY7045003.1"
                     /translation="MEVWPGGPYPLGASYDGAGTNFALFSEVAGSVELCLFDAAGAET
                     RIQMIERDALVWHTYLPGVAPGQRYGYRVNGPYDPKRGLRCDPSKLLIDPYAKALEGA
                     IDWAPACFSYSFGNPAQRNTDDSAPHTMKSVVISPYFDWQEDRAPRHPYHETVVYEAH
                     VKGLTKTHPDIPAAIQGTYAALAHPAMIDHLTDLGVTAIELMPVHQFVNDSNLVDKGL
                     SNYWGYNTIGFFAPHNAYSSSGQVGEQVQEFKAMVRELHRADIEVILDVVYNHTAEGN
                     HLGPTLGFRGIDNTAYYRLVDEDLSYYYDTTGTGNTLLMRNPHVLQLIMDSLRYWVIE
                     MHVDGFRFDLAASLARQFHEVDRLSVFFDLVQQDPVVSQVKLIAEPWDVGPGGYQVGN
                     FPPRWTEWNGKYRDTVRDYWRGEPASLGEFAARLSGSSDLYESDGRAPYASINFVTAH
                     DGFTLRDLVSYDVKHNDANGENNNDGENHNRSWNCGVEGPTDDIAVLALRSRQQRNFL
                     ATLLLSQGVPMLLHGDEFGRTQRGNNNVYCQDNAVSWVDWSAVDGELLVFTQRLTDLR
                     ADHPVFRRRRFFTGRTADDGLPDIAWLGPDGTPMDSEDWYQPRVRPLAVFLNGEGITE
                     PGLRGEEIVDDSFLLLLNPGGDEVAMTLPAAPFGDRWQLLFDTSATDPFDVAASVAQV
                     ARATRDVGAHSIVLMRKV"
     gene            15052..17448
                     /gene="treY"
                     /locus_tag="VH419_15145"
     CDS             15052..17448
                     /gene="treY"
                     /locus_tag="VH419_15145"
                     /EC_number="5.4.99.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020392720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047470 - (1,4)-alpha-D-glucan
                     1-alpha-D-glucosylmutase activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HEY7045004.1"
                     /translation="MRDRLTATYRLQLHRRFTFDDAAAIVPYLGELGVSHLYLSPVLQ
                     ATPGSMHGYDVVDHGTVSSELGGREGLERLADRAHDHGLGILVDVVPNHMAIPSPEYL
                     NHQLWDVLERGRSSAYAHWFDVDWDLCAGRLGLPLLGAELGTVLAAGELRLDVHDGQP
                     VVRYGEHVFPVADGTEDGDVAQVLARQHYLLGSWRDNAGLLGYRRFFDVDTLIAVRVE
                     LDDVFDATHAVLLDLYAEGVVDGFRIDHPDGLADPEAYLARLADATHGAWVAVEKILI
                     ADERLPDAWQASGTTGYDAITAVQTALSPPSGRRLDDLWSGIAGQASVADVELAAKRQ
                     VVSTLLAPEVARLARVVREISAVPRSDSWPAAIAELLVHVDVYRVYLRPGTTPTPAQV
                     DHLDRIVERARRSRPDLSVELSWLRDRLCDVASDDLRVQELLVRFQQVCGPVMAKGVE
                     DTTFYRHNRLLALNEVGGDPDSLLAPGPERLHEWAAHQQRRHPLGLTTLSTHDTKRSE
                     DVRARLLALAADPDSWEELWSEVWARATARGVDGPTAYLVFQTVLGAWPIDEGRLTEY
                     LTKAMREAAQHTSWTDPQHGYEDRVHGLARDCLSDAMFTVGVERLLGIHAEAISTVTL
                     GQKLIQLTLPGVPDCYQGCESVALTLVDPDNRRPVDHPSLHARLVRLSRAGGLSAGSD
                     ESLVDKKLWVTARTLRLRRDRPELFGPGSAYRPLDTSADVVGFVREGGAAAAATVVRR
                     VRAGAEGEVESEVLLPAGRWTDVLTGRPHSGGRRATAGLLIDLPVALLVSQDAARVAY
                     "
     gene            complement(17442..18044)
                     /locus_tag="VH419_15150"
     CDS             complement(17442..18044)
                     /locus_tag="VH419_15150"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEY7045005.1"
                     /translation="MVDVVARPPVSLRPERPGEGAIVDSMVVSAFEDDRLAGLLSALR
                     GSDDAWVEGLSFVAELDGEIAGHVLFTRSLLDAPPRLVNVLVLSPLSVRSDLRGRGIG
                     SALVRHGLDQVRRRTEPLVFLEGSPAYYGRLGFVPGGGLGFRKPSLRIPDAAFQVMTL
                     PAYEPWMTGTLVYADAFWRHDCVGLRDEPTSAAKPQPSDQ"
     gene            complement(18057..19127)
                     /gene="rsgA"
                     /locus_tag="VH419_15155"
     CDS             complement(18057..19127)
                     /gene="rsgA"
                     /locus_tag="VH419_15155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012920950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEY7045006.1"
                     /translation="MVEPSSTLRELGWDDGWHALAQHASCPGIPGRVSRVDRGVCTVL
                     TEHGPVRASWSGALLEAAAADTTEAPCTGDWCLVRCWPDGPVTVETVLERRTAVIRAE
                     VSRSSRGQVLVANIDVIAVVVALHPEPNLSRVERLLTLAWESGATPVILLTKADLVRD
                     AAGVAEDVRASCPGVDVIVCSTVTGAGLDELRTTLGPAGTLGLVGASGHGKSSLTNAL
                     VGTDVLVTKEIRDDGKGRHTSVRRELVTIPGGGAIIDTPGLRSVGLQRAEEGLAATFP
                     DVDAFTERCKFRDCSHEREPGCAVREAVARGELSQRRFESWLRLGRELARMEARADAR
                     LRAELLKKSKQATQQLRQRHRR"
     gene            complement(19208..20194)
                     /locus_tag="VH419_15160"
     CDS             complement(19208..20194)
                     /locus_tag="VH419_15160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEY7045007.1"
                     /translation="MAAETVGGEPASRTSRDWLVSFRSVSWRLLTATVGTCFRYRVTG
                     LAAEAAFFAVLSLPPLVFGLAGSIYFIVGKVDPEQISYLRGEIIDLSGQVLTPESVHK
                     IVVPTVNTVLQQPRFDVVSVGFVLALWSGSRALNVFVDTITIMYGLGGRRGIIRTRML
                     SFTLYVIGLVVGVVVLPLVLAGPRLVDQLLPQSADVLNTLYWPVVIVLSVAFLTALYH
                     LSVPVRTLWLHDVPGAVLAFTMWVLGSYLLRVVLETATTSIYGPLAAPIAVLIWLYLT
                     ALAVLIGAAVNAAFDRVFPEPSTVRARLEMLRRLGRKRSTNDPRPATDVRGI"
     gene            20287..21756
                     /locus_tag="VH419_15165"
     CDS             20287..21756
                     /locus_tag="VH419_15165"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002546127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA];
                     GO_process: GO:0035999 - tetrahydrofolate interconversion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine hydroxymethyltransferase"
                     /protein_id="HEY7045008.1"
                     /translation="MTDTAPGPVLDDDALIKTASTAYQSSLEVMAAVEPRIADATRAE
                     LADQRASLKLIASENYASPAVLLTMSSFLSDKYAEGTIGHRFYAGCQNVDTVESVAAE
                     HARELFDAPYAYVQPHSGIDANLVAFWAILATRVESPALQNAGAKTVYDLSEEDWETL
                     RHELGNQRMLGMSLDAGGHLTHGFRPNISGKMFHQRSYGTDPETGLLDYDRVAAAARE
                     FRPLILIAGYSAYPRRINFAKMREIADEVGATLMVDMAHFAGLVAGKVFTGDEDPVPH
                     AHVVTTTTHKSLRGPRGGLVLATAEYSDAVDRGCPMVLGGPLSHVMAAKAVALAEARR
                     PEFQDYAARIATNAQALAEGFLTRGATLVTGGTDNHIVLLDVSSFGVTGRQAESALLD
                     SGVVTNRNSVPADPNGAWYTSGIRLGTPALTTRGFGPDEFDRVAELIVEVLTNTSADA
                     AKSGPSKAKYTLADGVADRVKAASAELLDRYPLYPGLEL"
BASE COUNT         3693 a         7720 c         7499 g         3296 t
ORIGIN      
        1 cagtgattca cacatgaagc cgacggtggg tccgccgatg atgagcagga acgcgttgcc
       61 gtagtcggac tggccgatct cctggcgttg ggagaggtcc gccgcggcga ggaggagccc
      121 ggcgacgagg ccgccgaccc atctgtagtg gatcgaccag gcgatcagag cgccgatgac
      181 ccagaacccg ggcacactgg cgctgaactc tgcccccttt acgagcggtg tgacgagaag
      241 cagcgccacg gcaagcgcca ggtcggtagc caggagcggg aaggtacgcc gccgcggctc
      301 ggcgtacgcc cacgtcgcga aaccggtcca caggaccatc acgacgacgc aggcggctcc
      361 tgcggccgaa tgctggaagg tgtcggcccg gtagatgttc agcgcgacgg cgttgacgag
      421 gaggacgacg cggagcacca cgagagcgct gaacagccgg ttctcgacgg cgatcgccgc
      481 aggggacgac cacgagttca cacgcggcat tgtcgcacca ccggtgcgtc ggatccgctc
      541 tcggcaattc agatgcgcga ggcagccacg agcgagacct gagtatccgt accgatgtca
      601 gctcgtgtcc ggcccgatga gatgggaggc atggaaacgc caccacccgc cgcgaagccc
      661 tgcggcgacg accatcgagg ggttatgacg atgactggag cctctgagag cccgggagga
      721 tcggcgcccg tcccgtggac cccggcgccc tactcacgca acggcgaccg acacgtgccg
      781 gactccgggt ggcgtcgttg gacaccccga atcttggtca cgctgctgat cgtcggtggg
      841 accgcggccc tttctgccct cggcttggcg ttcctctacg gaatgaaccc gatgggcgat
      901 gaatgggtgt gcggcgacgg cgaggcgccg acaaccacgt actgctaccc gacggacgaa
      961 cccctcccgg ccggcgtgca gtgggatccg ctcggcaacc ggccgatgcc ctacaactgc
     1021 gacaagaacg gctggaccct catcgagcac aaccgcggcg acaaacagga ctgcctcaac
     1081 aacaacctgc cgatgcccgt cggatggcac gaggtgaagt gatcccggca ccgtccacca
     1141 ccggcatccc gagcgacggg acgtcagcgc atcgtcgcgg cccgccactt cggatcccgg
     1201 ccggtgaacc cgatcaggcg cgtctgcggg tcggcgatag cgggcaccgg gaccctcggc
     1261 ccgaactggc cggagtcccg catcaggtcc tcgatcggct ccattcccga cagcagtacg
     1321 tcgcacaggt ccgggtcgag cgcgtcgtcc tgggacgtgg cacgggccag gtcccaggtg
     1381 tgcatgaaca cgtcggaggt gtagaagcgg tcgatcgcct gcgcgagcgg catcgagccg
     1441 gtgtgccggt tcgcgaactc cacaccggcc gtcgcggggt cgtcgaggag accctggacc
     1501 gcgcgctggt gggcgtccca ggcgggtgcc ggctgctcgg ccggcgacgg cacctccggc
     1561 agtcggacgt cggagccggc agccacgaat ccgggcagcc acccgaccag gtgcacgacg
     1621 acgtcccgtg ccgtccagcc cgacaccggc gccgcggccg accagtcttt ggtgccgtgc
     1681 acgcgtcggg cgaagtcgtc ggcgatcaac cggtggcggc gagccggggc ggcggtgacc
     1741 agctcagagg gagccatcgg cgagcatccg gtcgatctcg gcgtacccgt cgttgacgcc
     1801 gacctccatg ccgctgcgca gccaggcgtc gcgaccctcg aacgagtcgc acagggagac
     1861 agcgtggagg cgggtacgac cgtcgccgag atcttcgaag gtcatcgtct cgagtgcgac
     1921 gtcgtcgggc atgccctccc acgtgaacgt ctggacgatg cggtcaggac ggacggtgtg
     1981 gaagcagccg cggaacgcct gctggtcgtc gtctcggctg tcgaggaaac ggaacgagcc
     2041 tccgtcggtc gcgtcccagc ggtcgatgac cgccgtccgg tcggccggcc cgatccaccg
     2101 ggcatagagc tccgggtcgg tgtgggcacg gaacagctga tcgggggtgc ccgcgaagtc
     2161 gcgccagatg tggatggcgg ggattgtcgg gtcggcctcg atcacggcct ggtcgtagcg
     2221 ggtgtcggtc gatgcggtgc tcatgatgcg gtcccctccg tcggagtctc ggtctgttcc
     2281 tcgtccagtt ccgcgagtac ggcgtccaag cgctggtagc gctcttccac gcgtcggcgg
     2341 tagcgctcga tccacctggt catcaggtcg aacacctcgg cttccaggtg caccggacga
     2401 cgctgagcct cgcggctgcg cgtgaccagg ccggcctcct ccagcacctt caggtgcttg
     2461 gacacggcct gcaggctcac ggcgtacggc gcggcgagcg tgccgacggt ggcgtcaccg
     2521 tcggtgagcc gggccaccat gtcgcgccgg gtcgggtcgg ccagggcggc gaagacccgg
     2581 gagagctggt cgggcatctc gtcgcctttc gtcaaccatt cggttgagca cgactctatc
     2641 caggcacctg cgacttcgtc aaccaattgg ttgacgaagg tcggcacgga aaatcaggtg
     2701 cgggaagggc tctcagtcgg tgagatacgc gatcagcgcg tcgtcgaccg gctcactgac
     2761 gaaaccggtg gcggcgatct tgtccagcga caacaggctg caccgtggac gcggggcgga
     2821 cgaccggccg gcgtcgtact cctcggtgct gaccggccgg atgtcttcag gatcgcgacc
     2881 ggagagctcg aagacccgtc gcgcgacgtc ggcccacgac tgcggcggcc cggcattggt
     2941 gacgttgtac gtcccgggcg ccgcatcgac ctcgagcagg tgccgggtgg ctcgcgcgag
     3001 ctcggaggtg aacgtcagtc ggccgacctg atcatcgacg acggacggac tgacgccgtc
     3061 ggcggcaagc cggcgcatgg tccggacgaa gttgttgccg tcgccgacca cccaggacgt
     3121 gcgcaccagg tagtggcgcg ggcaggtccc caccaccagg tcacccgcag ccttggactg
     3181 gccgtaaacg ccgagcggtg agagagcctc gtcctctcgg tgaccgccgt cgacctgcgt
     3241 gccgtcgaag acgtagtcgg tcgagtagtg gaccagcgtg aggcggtgtt ccgttgcgat
     3301 tcgagcaagg gtggtcggga tcccggcgtt cgcccgccag gccgaacgtc ggccctcagg
     3361 ggtctcggcc gcgtcgacgg cggtgtacgc cgccgcgttg aggacgaagc cgtactcgtg
     3421 ccacggccag gccgcaacgg cagcctcgtc ggtcaagtcc agctcgtggc tgccggccgt
     3481 cgtcgcgtcc gggaactccg ccgccagtgc ccggccgagc tgcccgttgc ttccggtgac
     3541 gagagtcctg cgcggctgca acgggacgac ctcggcgagc cgaggattgc gggtgcggtc
     3601 cttctccgag acgtctgcct ccgccagcgg gatgggccac gcgatggctg ctgtggggtc
     3661 gtcgagcgcc aacgccgggt actcctggcc cgggcgccag tggtcgttga cgaggtagca
     3721 gtacgtcgtg gcagccgcca gggcctggta ggagttgccg accccgcgcg ggacgtacac
     3781 gcacaccgac gggtcgacct ccgtggtgaa cacggtgccg aacgtcgggc cggtgcgcag
     3841 gtcgacccag gcgccgaaca cccgcccggc cgcgaccgtg acgaacttgt cccacggctc
     3901 ggcgtggata ccgcgggtgg taccccggtt ggcgttgtag ctcatgttgg cctgcaccgg
     3961 cccgaagtcg ggcagcccga gcgcggtcat cttctcgcgc tgccacgcct ccttgaacca
     4021 cccccgcgca tcctcgtgca gcggcacgcg gacgaccaga aggccgggta tgggcgtggg
     4081 ctcgacggtg atgtcggaca acgtcactgc cccttcgctg cgtacgccgc ctcgatcgca
     4141 tccttgtggg gccaccacca ggcctcgttg tcgcgatacc agtcgatcgt ctgcgcgaga
     4201 ccggcttcga agtcgccgaa ccgtggcgtc cacccgagct cgctgcggag cttcccggag
     4261 tcgatcgcgt accgcaggtc gtggccgacc cggtcggtga cgtggtcgaa gtcgtcctcg
     4321 tccctgccca tcaggcgaag gatcagccgc accaccgcga tgttcgaccg ctccccgtcg
     4381 gcgccgatga gataggtctc acccagccgg cctcgctcca ggatggtcag caccgccgag
     4441 ctgtggtctt cggtgtggat ccagtcgcgc acgttctcac cggagccgta cagcctggga
     4501 cgcccgcccg cgagcacgtt ggtgatctga cgcgggatga acttctccac gtgctgccag
     4561 ggcccgtagt tgttggagca gttcgagatc gtcgcccgaa tcccgaagct gcggacccac
     4621 gcgctgacca ggtggtccga gcctgccttc gacgccgagt aagggctgct ggggcggtac
     4681 ggcgtgtcct cggtgaaccg cttcgggtcg tcgagcgcga ggtcgccgta cacttcgtcg
     4741 gtcgagacgt ggtggagtcg gacgtcggcc ttgcggacgg cctccagcag cgtgaaggtg
     4801 ccgacgaggt tcgtgtggac gaacggggtc gggtcgcgca gtgagttgtc gttatgcgac
     4861 tctgcggcga aatgcacgac ggtgtcgtga ctcgcaacca gcggctcgac cagcgccgca
     4921 tcggcgacgt cgcccttcac gagcgtgaaa cggtcctccg gcaacccctc gaaggcttcc
     4981 gggctggccg cataggtcag cttgtccagc acggtcacga tcgcgtcggt gttccgcatg
     5041 aggtggtgca cgaagttcga gccgatgaag ccagctccgc ctgtgaccag gatccgattc
     5101 acgcccgcct gtgcatggac cacaatgagg cacaccctac cgggacgacg gaaggtggac
     5161 atgcgcggga tcatcctggc cggcggcacg ggcagccggc tgcacccgat cacccgggcc
     5221 atcagcaagc agctgatgcc ggtctacgac aaaccgatga tctactaccc gctctcgacg
     5281 ctgatgctgg cgggcatccg cgaggtgctg gtcatcacca cgccacacga gagcgagatg
     5341 ttccgtcggt tgttgcgcga cgggtcacag ttcggcatcg ccatcgagta cgccgtgcag
     5401 ccgtcgccgg acggcctcgc ccaggcgttc ctcatcggcg aggatcacct cgccgggggt
     5461 ccagcaggac tggtgctcgg cgacaacatc ttctacggct cggggctcgg gagccgactc
     5521 cgccggttcg ccgagatcga cggggccgcc gtcttcggct accgggtagc agacccgtcg
     5581 gcgtacggcg tcgtcgaggt cggtgcggac ggccgcgctc tctccttgga ggagaagccg
     5641 cagaagccac gcagcagcct cgccatcccc ggcctctact tctacgggcc cgacgtcgtg
     5701 gagcgcgccc ggacactgaa gccgtcggcg cgcggcgagc tggagatcac cgacctcaac
     5761 cggctctatc tcgaggaggg ccggctccgc gtcgagatcc tgccacgcgg atcggcgtgg
     5821 ctcgacacgg gcacgttcga cgacctcaac gacgccagca cgtacatccg ggcgctcgag
     5881 caccggcagg gcaccaaggt cgggtctccg gaggaggtcg cctggcggat gggctacatc
     5941 gacgacgacc agctcggcga gctcgcccag gacatgctca agagcggcta cggcaggtat
     6001 ctcctcgacc tcctcgccga ggagcggggc tcctgaccgg aactgcgacg ggcacccctt
     6061 agggtggtgg gcggtccgcc ggggcctgtc cgtcgcacct cgtcccgggc catctatgct
     6121 gccgtgactc gagagaggac tgcacaggtg cgcaaacacc agttgctgcg ggtgatccac
     6181 gagcgcctgc ggccgagaac ctacttcgag atcggcgtcc gcaagggtgt cagtctcggg
     6241 ctgtcgcgag tgccgagtgt cggcgttgac cccttctaca tggtcaccga cgagctgcgc
     6301 tgcgacgtgc acctcgtgcg cacgaccagt gacgagttct tcgcgcgcaa gcacccgttc
     6361 gcccatttca agcagcaggt gatcgatctg gccttcatcg acgggatgca tctcgccgag
     6421 ttcgcgctgc gcgacttcat caacaccgag cggcactgcc atccagccag cgtgatcgtc
     6481 atcgacgacg tgctgccccg caccgtcgag gaaggcggtc gtggccgttc cgggagcgcg
     6541 gacggcggtg cctgggcagg cgacgtctac aagatcatcg acgtgctgcg ccgaaaccgc
     6601 cccgacctgg tcgtgctcga gatggacacg cagccgaccg gtaccctcgt catcctcctg
     6661 ccggacccca ccaaccgggc attggccgcg gcgtacgacg aggcggtccc cgcgatggtg
     6721 gtgcccgacc cgcaggacgt cccggcgcac atccttcgcc gaaccaacgc gcgcgacccg
     6781 gaaaagctga tcgccgcgcc gatctgggac gcactccgtg aggccaggag gctttcgcgg
     6841 accgaggctg gtgcgcaggt ccaggaagca ctcgcggctg caggtctcac cgacgaggcg
     6901 gtactgcgat gagccgcaac acgatgcccg tcgacgaact gttgagctgg gcacgccgcg
     6961 agggacctgc gatccaggtc ggtctgcgtc tcccaggtgc ggaccttgca cccggcaaga
     7021 ccacggtgcg cctgactcgc cggaagggtg agctccaagt cgacgcggat gccacgggcg
     7081 agtcggacgc agtcacgctc gcgttcacgg tgccacgcac gacacttgcg ccgcccgcgt
     7141 ggaggatcgc cgtgcagacc ggcgccgacg gccggttcca gccggtcgaa gcgcggctcc
     7201 tggccccgaa agatcagccg gtcgcggtac tgcctgggcc tcccccggac acgaagatgc
     7261 cggctccctc cccgcgtcgg ccaggtaggg ccatgcggac gccggtcgca acgccgacac
     7321 gccgagtgct ccggcggggc cgaagcctcg cgcgggccgc ccgacgggca gcgcgaaagc
     7381 tcaccgcggg ccgcggcagc gaccgatcgg ccgatcgacc tggccgacgg cctggcaccc
     7441 aacccgaccg gccggcgctt cggctggacg gacccgcgct ccgggcggta cgggcggcag
     7501 ggaaacaccc ggtcgtcgta ctcgcggacg cctcgacgga gcacgcggtc tcggcgtggc
     7561 tcccggcgct gcgtggcact gacgtcacga tgtttcggat cggggccgac agtccccgtg
     7621 ccgcgactgc tgccgacgtg gtcgacgttg ccgacactgc cgatgttgcc gacgccgccg
     7681 acgctgccga cgtggtcgac gcgaacgacg tcgacgacgt cgaacggagg ctcttcccga
     7741 ccgaggcgcc ggcggcgctc gtggtggctc tgtcaccggc cacgcttcga tcgctcgcca
     7801 ccgaccatgt ccagctgctc gtccggctct ttccccacct gcgccacggc ggagcctatg
     7861 tcgcgtatgc ggtgccgacc ggcgggccct ccgcctccgc ccgcgaccat gccatcgcca
     7921 cgttccggcg tgctctcgag gagagcgcag gggacctcac ggccggctcg ccgggtggca
     7981 ggaaaccgcg gatgcggggc atccgcgatg tcgtggtgcg accaggcttc gtgctgctgc
     8041 gcaagaaccg cagggacgca ctccacgtgc gcgaggaaca ggtcgccgac ctgctgccgg
     8101 cccgggagcc gggcaccgag gtgtcgatcc tcgaagtccg cccgagcggg tcgttgcagg
     8161 tcacagcgac cgagatcagc tacggtccgc ggcaggcgga accgtggccg aaacgactgg
     8221 actaccccga gctgaccctg cgccactacc gcggagacct gatgagcatg ggttccatgc
     8281 tgctgaccgc cgagtccacg atcctgcctg agtcgttccg gtggccgcat cgctcgaagc
     8341 tgggtcaggc cagcgttcgc tcggcagggc cgcacttcag cacggttccc cgggtcaagg
     8401 gtgcgccgat cctcgagggg gactactact tcctcgactg tctgttcagc gggcacttcg
     8461 gccatctcct caccgaagtg gtctgccggt tgtggggctg ggaggcggcc cgccgggaac
     8521 tgccgaacgt caaggcgttg ttccacacca acccacgtcg gggtcgccac ggggcgctcg
     8581 aacgaaagct ctttgcggcg tacggcgtcc cggaggctga tctcgtctcg acggaccggc
     8641 cggtgcggct gcgaagtgtc gtcggtgcat cgccgatgtg gcacaacgcg ccgccgttct
     8701 acgtccaccc ggacatccag gagacgtggg cccggctcac gtccgggctg ctcgacggca
     8761 ggccaccggc tgagcacgag cgcatcttcg tctcccgcgg cgacgacctc aaccgacgac
     8821 gctgccgcaa tcagcccgag gtggaacgct tcttcgccga tcgcggcttc cacgtcttct
     8881 atcccgagcg gctgccgttg gaggagcagg tggcgttgtt cgccggcgcg cgggtggtcg
     8941 cgggcttcgg aggctcggcg atgttcaaca tgctgcactg ccgccgactg gagaagacca
     9001 tcgtcatcag ccacaacagc tacgaggccc gcaacgagaa cctgttcatg tcggtgctcg
     9061 gcggcgagct ccactacttc tggcaggagt ccgacgtgtc gctcaccggg ggcgccacca
     9121 acaggcagaa gcggcggtcg tcgtacgcct tcgacttcga gaactacggc accgatctgg
     9181 cgcgggtgat cagccagccc tgaccagcac gcaggacact ggatctgatc caccaagcag
     9241 cggaccctac gctggcgaga tgatcaccag cgtccactca ctcatctact ccgacgacgc
     9301 tcccgccacc cgggccttct tccgcgacgt gctcggttgg ccgtacgtcg aggacacgga
     9361 gtccgatgcc ggctggctca tcttccagac cgggccgagc gagttggggg ttcacccgac
     9421 atccgggatg cacgagggtc ggccatggtc ggcaacccgg catcacgcga tctcgctgat
     9481 gtgcgacgac atcgcggcga cccggaagga gctcgaagac aaaggcgcga cgttcagcgg
     9541 agagatcgcc gatcacggct acggccaggt gacgatgctc caagttcccg gcgccgacga
     9601 catccagctg tacgagccga gtcatccgac ggcctacgac ctctgatccg cgctctggag
     9661 tcagccagag atctccaagg tgggggtctg gctgccgccg acgcccagga catggtcgat
     9721 ctccctctgc gtcagctggc ggtcagcact cgaggccacc accatcaacg accccagcag
     9781 ctcgatctcc tcgagcaact gaacgtcctg caggacaacg tcgtccttca tggagcctgc
     9841 cttctcgtcg ggcaccgcag ccaatgacgc tcggcctcac agaccaagca cgtcgaacct
     9901 cccgagacca acgctgccgc atgaccggcc gtggtgagga ccacccacgc cgaagatggc
     9961 ccgaagtgac cactcggcat cacccgcgag acgtccatgg ccgcgggcgg ccctggcgca
    10021 ggtggtgatc gacgccaggg cctggaccgg tgacacaccg cggatggcag ccggtccttc
    10081 cttgcggtat gcccaggacc ggctcgctta gtcgtccggc cctgttagtc gtccggcccc
    10141 gctgtgtgca ccggcctgcg tcacttgccc cggcgtccgc ccgggtcgag actggttgat
    10201 gacggtgtga tcgcacccat accgacacga tcgaggttct cccatgcgca cccatgcata
    10261 tcgcacccct cgaaggacgg ccggctggac ggttcgacgg ctgcctgcca tcgcagccct
    10321 catcctggcg gcggccttcc ttcccgcgtg tacgtcgagc tcggaccacg acgcgacgaa
    10381 caagacgaac aatcagtcag gcgagtcgga gcagtccgag gacgagcacg ccgaagccga
    10441 aggcgaagag cctggcgaag aagaaggcga agagggcggc gagggctgga acgaggacat
    10501 cgagcatgcc gcagaagcgg caggcccgga gcttccggag gactccttcc tgttccagcg
    10561 gctgatgacg agcggctccc ccgacctcaa cgccttcggt cgggccaagc agcaggccac
    10621 cgcgctgacc gcgctggcgg ccaggacgac tccgcagctg aagcagcggc gatgggagtt
    10681 cctcggcccg gagaacgtcg gcggccgcgt taccgacggc gtggtcgacc ccacgagacc
    10741 caacacgatc tatgtcgccg cctccacgaa cggcgtgtgg cgctcatccg acgccggtca
    10801 gacgttcacc tcggtgtggc ccgacgacat cacacactcg atgggagccc tggcgatcgc
    10861 accgaacggc atgctgtacg ccgggaccgg cgagacgaac cccggcggtg gcagcatcac
    10921 gtacgggggc aacggcgtct accgatcgac caaccacggc ctcagctggc aacgagtcgg
    10981 cctgacgaga tcggggacga tcggccgcat cgtgatcgac ccgaaggcgc cggaccgcat
    11041 ctgggtcgcg gtcagcggca acctcttcac ccccggcggc cagcggggcg tctacgagtc
    11101 gaccgacggt ggcgacacgt ggaagcgctc actcagacca ccgaacgagc tcaccggagc
    11161 gaccgacatc gccatcgacc cggccaacag ccgccacctc atcgcgacga tgtgggacca
    11221 catccgcagg ccgagcgcac ggatctacac cggcatcggt tccggggtct gggagaccca
    11281 caacgccggt cgcacgtgga aacgcctcgg gccggcgcag gggctgctgg caccgtcggg
    11341 caacaccggg cggatcggcg tcacgttctc accggccgac gctgaccggg cctggatcat
    11401 ctactccaac gacgagaccg gggccttcca ggacttcttc cgttccgacg acaacggtgc
    11461 gagctggcag cggccgccgg gaaccgacca gctggccgac tcacagagcg tctacggctg
    11521 gtggttcggt cgcttgtacg ccgacccgga ggacgccgac cggctgtacg tcctcggtct
    11581 cgacctctgg gagacgcggg acgcgggcgc aacgttcgac accgtgccgg acatccacgt
    11641 cgaccagcac gtcgtgatgt acgacccggc tgtccccgac cgcacctacg tcggcaacga
    11701 cggcggcctg tacgtcagtg aggacaacgg cgccagcttc accaagtccg aggaccagcc
    11761 gtggtcgcag tactccggcg tcgacgtgtc cgagcaggac cagtcccaca tcgtcggcgg
    11821 tctgcaggac aacggcacac aagccacctg ggtggccgac gggtcggact ggggatcgat
    11881 cttcggtggc gacgggcagc gctcgctcat cgacccgacc gacgctgaca actactacgc
    11941 ctgcgcgcag tacggcgtct gcgcgggctt cgaggacggc tcgtcgttcc agatgcagtt
    12001 cacgtcgaag cgattcccgt acttcatgca gtacgcgttc aacccgaaga acaccgacgt
    12061 catgtacggc ggcggcagcg agctgaacaa gtccaccgac gaggggcaca cctggacggt
    12121 gatcagcgac gacctcggca agggcgaggc tggtgacgag cccaacccgc tctaccgcaa
    12181 ccactacggc accgtctccg cgctcgcggt ggcgccgagc aggccacgga tcgtctgggc
    12241 cggtaccgac aacggctacc tgtatcgcag cgcggacgcc ggcgccagct ggcaggaact
    12301 gccgaacccc gtccgcccga agctgtggat ccagcgcctg gtggtcgccc cggacaacgc
    12361 caggaccgtg tacctgacgt tctccggcta tcgggaaggc gacaactcgg cgtacgtcct
    12421 ccggacccgc aacaacggcc agacctggac cgacatcacc gccaacctcc cgcacgcccc
    12481 ggtcaacgac ctggccgtcg tcggtgaccg gctgtacgtc gccaccgatg tcggcgtctt
    12541 cacctcaccc gccagccgac cacgctggac ggcatacggc agcggcatgc cacagctggt
    12601 gacgaccgac ctgcggtatg tgccggagaa cgcgaccttg tacgtcgcca cgttcggcat
    12661 gggggtgtgg tcgatcaagg cctgagatgg tccgcgggct ccggcggtca gcgggttccc
    12721 gcggtcggcg gtttccggcg gtcggcgggt tccggcggtc gagcccgtcg agaccctcga
    12781 cttccggcgg tcgagcctgt cgagaccccg agacccctcg atctcgacaa gctcgatcag
    12841 cgggccacgg cgatcagcgg accacggcga tcagcggggc tcggtagggt ctccccgaac
    12901 gaaggagacc gatggaagtc tggccgggag ggccctaccc gctgggcgcg agctacgacg
    12961 gggccgggac caacttcgcg ctgttctccg aggtcgcagg gtccgtcgag ctgtgcctgt
    13021 tcgacgctgc aggcgccgag acccggatcc agatgatcga gcgcgacgca ctcgtgtggc
    13081 acacctacct gcccggcgtg gcgccgggac agcggtacgg ctaccgcgtc aacggaccat
    13141 acgacccgaa gcgtggcctg cgctgcgatc ccagcaagct gctcatcgac ccgtacgcca
    13201 aagcactcga aggcgccatc gactgggcgc cggcgtgctt ctcgtactcg ttcggcaacc
    13261 cggcccagcg caacaccgac gactcggctc cgcacacgat gaagtcggtc gtgatcagcc
    13321 cgtacttcga ctggcaggag gatcgggcgc cgcggcaccc gtaccacgag acggtcgtct
    13381 acgaggcgca cgtcaaggga ctgaccaaga cccaccccga catcccggcg gcgatccagg
    13441 gcacgtacgc cgccctcgcg catccggcga tgatcgacca cctgaccgac ctcggtgtca
    13501 ccgcgatcga gctcatgccg gtgcaccagt tcgtcaacga ctcgaacctg gtcgacaaag
    13561 gactgtcgaa ctactggggc tacaacacga tcgggttctt cgccccgcac aacgcgtaca
    13621 gctcgtccgg tcaggtcgga gagcaggtgc aggagttcaa ggcgatggtg cgtgagctgc
    13681 accgcgccga catcgaggtg atcctcgacg tcgtctacaa ccacaccgcg gagggcaacc
    13741 atctcggccc gacgctcgga ttccgcggca tcgacaacac ggcgtactac cggctcgtcg
    13801 acgaggacct gtcgtactac tacgacacca ccgggaccgg caacacgttg ctgatgcgca
    13861 acccgcacgt cttgcagctg atcatggact cgttgcggta ctgggtcatc gagatgcacg
    13921 tcgacggatt tcgcttcgac ctggcggcca gcctcgcccg ccagttccac gaggtcgacc
    13981 ggttgagcgt gttcttcgac ctggtccagc aggatccggt cgtctcccag gtgaagctga
    14041 tcgccgagcc gtgggacgtc gggccgggtg gctaccaggt cgggaacttc ccaccccgct
    14101 ggacggagtg gaacggcaag taccgcgaca cggtccgcga ctactggcgc ggcgagccgg
    14161 cgtcactggg agagtttgcc gcccgccttt ccggttcgag tgacctctac gagagtgacg
    14221 gccgggcgcc gtacgcgtcc atcaacttcg tcaccgccca cgacggcttc acgttgcgcg
    14281 acctggtgtc gtacgacgtc aagcacaacg acgccaacgg tgagaacaac aacgacggcg
    14341 agaaccacaa ccgatcctgg aactgcgggg tcgaaggccc gaccgacgac atcgccgtgc
    14401 ttgcgctacg gagcaggcag cagcgcaact tcctcgccac cctgctgctg agtcagggcg
    14461 tcccgatgct gctccacggc gacgagttcg gacgcacgca gcgcggcaac aacaacgtct
    14521 actgccagga caacgcggtg tcgtgggtcg actggtctgc ggtcgacggt gagctgctcg
    14581 tgttcaccca gcggctgacg gacctgcggg cggaccaccc ggtgttccgg cgccgtcgat
    14641 tcttcaccgg ccggactgcc gacgacggcc tgccggacat cgcctggctc ggccccgacg
    14701 gaacgccgat ggactccgag gactggtacc agccgagggt ccgtccgctg gcggtgttcc
    14761 tcaacggtga ggggatcacc gagcccggcc tgcgcggtga ggagatcgtc gacgactcgt
    14821 tcctgttgct gttgaacccc ggcggcgacg aggttgcgat gacgctcccg gcagcaccgt
    14881 tcggtgacag gtggcagctg ctcttcgaca cgtccgcgac ggatccgttc gacgtggcgg
    14941 catccgtggc gcaggtggcc cgcgccacgc gggatgtcgg cgcacactcg atcgtcctga
    15001 tgcgcaaggt ctgatgtgcg agacgtcgcc gaacccggtg acgggcacgc tgtgcgtgac
    15061 cggttgaccg cgacgtaccg gctgcagctg caccggcgct tcaccttcga cgacgcagcg
    15121 gcgatcgtgc cgtacctcgg tgagctcggt gtctcgcacc tctacctgtc cccggtgctg
    15181 caggcgacgc cgggctccat gcatgggtac gacgtcgtcg accacggcac ggtgtcgagt
    15241 gagctcggcg gacgggaggg tctcgaacgg ctcgccgacc gggcgcatga ccacggtctg
    15301 gggatcctgg tcgacgtggt cccgaaccac atggcgatcc cgtcgccgga gtacctgaat
    15361 caccagctct gggacgtgct ggagcgtggc cgctcatcgg cgtacgccca ttggttcgac
    15421 gtcgactggg acctgtgcgc cgggcggctc ggcctgccgt tgctcggtgc agagctcgga
    15481 accgtcctcg ccgcaggtga gctgaggctc gacgtccatg acggccagcc agtcgtgcgc
    15541 tacggcgagc acgtgttccc tgtcgcggac ggcaccgaag atggcgatgt ggcgcaggtc
    15601 ctcgcccggc agcactacct gctcgggtca tggcgagaca acgcaggcct gctcggctac
    15661 cggcggttct tcgacgtcga cacgctcatc gccgtccggg tcgagctcga cgacgtgttc
    15721 gacgcgaccc atgccgtgct cttggacctg tacgccgagg gagtcgtcga cggcttccgg
    15781 atcgaccacc ccgatggcct tgctgatccc gaggcgtacc tggcacggct cgctgacgcg
    15841 acgcacggcg cctgggtcgc cgtggagaag atcctcatcg cggacgagcg gctccccgac
    15901 gcctggcagg cgtccggcac caccggctac gacgccatca ccgccgtgca gacggcgttg
    15961 agcccgccgt cgggccgccg cctcgatgac ctctggagcg gcatcgcggg tcaggcgagc
    16021 gtcgccgatg tcgagctggc cgccaaacgg caggtcgtga gcacgctgct ggcgcccgag
    16081 gtcgcccgac tggctcgtgt tgtgcgcgag atctcagccg tcccccgctc cgacagctgg
    16141 ccggcggcga tcgcggaact gttggtccac gtcgacgtct accgggtgta cctgcggccc
    16201 ggtacgacgc cgacgcctgc gcaggtcgac cacctcgaca ggatcgtcga gcgcgcccgg
    16261 cgatcacgcc ctgatctgtc cgtcgagctc agctggctgc gcgaccggct ctgcgatgtc
    16321 gccagcgacg acctccgggt gcaagagctg ctcgtgcggt tccagcaggt gtgcggtccg
    16381 gtgatggcca agggtgtgga ggacacgacg ttctaccgtc acaaccggtt gctcgcgctc
    16441 aacgaggtcg gcggtgaccc cgactcgctc ctggcaccgg ggcctgagcg gctgcacgag
    16501 tgggccgcgc atcagcagcg ccgccacccg ctcggcctga ccacgctgtc gacgcacgac
    16561 acgaaacgca gcgaggacgt gcgggctcgg ctcctcgcgt tggctgccga tccggacagc
    16621 tgggaggaac tctggtccga ggtctgggcg cgggccacgg cgcgtggcgt cgacggcccg
    16681 acggcgtacc tcgtgttcca gaccgtgctc ggggcctggc cgatcgacga ggggcgactg
    16741 acggagtacc tcaccaaggc gatgcgcgag gccgcgcagc acacgtcgtg gaccgacccg
    16801 cagcacggct acgaagaccg tgtgcacgga ctggcgcgcg actgcctgag cgacgcgatg
    16861 ttcaccgtcg gggtcgaacg gctcctcggc atccacgccg aggcgatctc gacggtcacc
    16921 ctcggacaga agctgatcca gctgacgctt ccgggagtcc cggactgcta ccagggatgc
    16981 gagtccgttg ccttgacgct ggtcgacccc gacaaccgcc gtccggtcga ccatccatcg
    17041 ctccatgcac ggctggtccg gctgagccgg gctggagggc tcagcgccgg gtccgacgag
    17101 tcgttggtgg acaagaagct gtgggtcacc gcgcggacgc tcaggttgcg acgcgatcgt
    17161 ccggagctgt tcggaccggg gtcggcctac cgtccgctgg acacgtcggc ggacgttgtc
    17221 gggttcgtcc gagaaggcgg cgctgcggcc gcggcgacgg tcgtacgccg ggtacgggcc
    17281 ggcgcagaag gcgaggtcga gagcgaggtc ctgcttcccg cgggacgatg gaccgacgtg
    17341 ctcaccggac gaccgcacag cggtggccgg cgcgccaccg ctggcctgct catcgacctt
    17401 cccgtggccc tgctcgtctc gcaagacgct gcacgagtgg cctattgatc cgacggttgc
    17461 ggcttcgcag ctgacgtggg ttcatcgcgc aggccgacac agtcgtgccg ccagaacgcg
    17521 tcggcgtaga cgagcgtccc cgtcatccac ggctcgtacg ccggcagcgt catcacctgg
    17581 aacgcggcgt ccgggatccg cagcgacggt ttccggaaac cgagcccgcc accggggacg
    17641 aagcccaacc gtccgtagta agcgggggag ccctcgagga acaccagcgg ttcggtccgc
    17701 cggcggacct ggtcgagacc gtgccgcacg agcgctgacc cgatgcctcg accacggagg
    17761 tcggacctga ccgagagcgg gctcaggacg aggacgttca ccaaccgcgg cggcgcgtcg
    17821 agaagactcc gcgtgaacag gacgtgaccg gcgatctcgc cgtcgagctc cgccacgaac
    17881 gacagtccct cgacccaggc gtcgtctgag ccacgcaggg cggacagcaa ccctgcgagc
    17941 ctgtcgtcct cgaacgccga gacgaccatc gagtcgacga tggcaccctc cccggggcgc
    18001 tcgggacgca gactcaccgg cggtcgagcg acgacatcaa ccatcggcgt acgacctcat
    18061 cgccggtggc gttgtcgcaa ctgctgggtc gcctgcttcg acttcttgag cagttcggcg
    18121 cgcagccggg cgtccgctcg cgcctccatg cgggcgagct cacgcccgag ccgcagccag
    18181 ctctcgaacc gtcgctgaga gagctcaccg cgcgccacgg cctcgcgcac ggcgcacccc
    18241 ggttcgcgtt catggctgca gtcgcgaaac ttgcatcgct cggtgaacgc gtcgacgtcc
    18301 gggaacgtcg cggccaaacc ctcctcagcg cgctgcagcc cgacgctccg cagtcccggc
    18361 gtgtcgatga tggcgccgcc gccggggatc gtgaccagct cgcggcgtac cgacgtgtgc
    18421 cggcccttgc cgtcgtcgcg gatctccttc gtcacgagca cgtcggtgcc caccagcgcg
    18481 ttcgtcaggg aggacttgcc gtgcccggac gcgccgacca ggccgagcgt cccggccggt
    18541 ccgagcgttg tccgcagctc gtccaggccc gcacccgtca cggtgctgca cacgatgacg
    18601 tcgacgcccg ggcacgaggc gcggacatcc tcggcgacac cagccgcatc ccggaccagg
    18661 tcagccttgg tgagcaggat gaccggcgtc gccccgctct cccaggccag cgtcagcagc
    18721 cgctccacgc gcgacaggtt cggctcgggg tgcagcgcga ccacgacggc gatgacgtcg
    18781 atgttggcga ccagcacctg gccgcgcgac gagcgggaca cctccgcccg gatgaccgcc
    18841 gtacgccgct cgagcaccgt ttcgaccgtc accggaccgt ccggccagca gcggacgagg
    18901 caccagtccc ccgtgcacgg cgcctcggtg gtgtccgcgg ccgcagcctc gaggagtgca
    18961 cctgaccaac tcgcgcggac cggaccatgc tcggtgagca ccgtgcacac gccgcggtcg
    19021 acccgggaca ccctgcccgg aataccgggg cacgaggcgt gctgcgccaa cgcgtgccag
    19081 ccgtcgtccc agccgagctc gcgcagcgtc gatgagggtt caaccacgcc atgagggtag
    19141 gtggccgccc gcacccccgc ccattgattt tccgcgcctt ggtctcctgg accggccgcg
    19201 cccaggctca gatgccgcgc acgtccgtcg ccggtcgagg gtcgttggtg ctgcgcttgc
    19261 gacccagccg tcgcagcatc tccagccgcg cacggacggt ggacggctcc gggaagaccc
    19321 ggtcgaaggc ggcgttgacg gccgcgccga tgagcaccgc cagcgcggtc aggtacagcc
    19381 agatgaggac ggcgatcgga gccgccagcg gaccgtagat cgacgtcgtc gcggtctcca
    19441 ggaccacccg gagcaggtag ctgccgagca cccacatcgt gaacgccagt acggcgcccg
    19501 ggacgtcgtg cagccacaac gtccgaaccg ggaccgacag gtggtacaga gccgtgagga
    19561 acgcgacgct gagcacgatg acgacgggcc agtagagcgt gttcaggacg tccgcgctct
    19621 gcggcaggag ctggtcgacg agtcgcggtc cggccagcac caggggcagc acgacgacgc
    19681 cgacgaccag cccgatgacg tagagggtga acgacagcat ccgagtgcga atgatcccgc
    19741 gacggccgcc gaggccgtac atgatcgtga tcgtgtcaac gaagacgttg agggcgcgcg
    19801 agccggacca cagcgcgagc acgaagccga ccgacacgac gtcgaaacgg ggctgctgga
    19861 ggacggtgtt gacggtcggg acgacgatct tgtgcaccga ctccggcgtg agcacctgcc
    19921 cggacagatc gatgatctcc ccgcgcaggt agctgatttg ttccgggtcg accttgccga
    19981 cgatgaaata gatgctgccg gccaggccga acaccagggg tgggagcgac agcaccgcga
    20041 aaaatgcggc ctcggccgcg agcccggtga cgcggtaacg gaagcaggta ccgacggtcg
    20101 ccgtgagcag tcgccacgag acagatcgga acgagacgag ccagtcgcga gaggtccgcg
    20161 aagccggctc gccgccaacc gtctccgccg ccatgggcca tacggtatcg accagaacac
    20221 gccgtctccg gcagccacga cgcggacgac ttcaccgagc gcatggccgg gcctacgctg
    20281 acctgcatga ccgacactgc tcctggccct gtcctcgacg acgacgccct catcaagacc
    20341 gccagtacgg cgtaccagtc ctcactcgaa gtgatggccg cggtcgaacc acggatcgcg
    20401 gacgccacca gggcggagct ggccgaccaa cgcgcgtcgc tgaagctcat cgccagcgag
    20461 aactacgcgt caccggcggt gttgctgacg atgagcagct tcctgtcgga caagtacgcc
    20521 gaaggcacga tcggccaccg gttctacgcc ggctgccaga acgtcgacac ggtcgagtcg
    20581 gtcgccgccg agcacgcccg cgagctgttc gacgcgccct acgcctacgt gcagccgcac
    20641 tccggcatcg acgccaacct ggtggcgttc tgggccatcc tcgccacccg ggtcgagtcg
    20701 cctgccttgc agaacgccgg ggcgaagacc gtgtacgacc tgtcggagga ggactgggag
    20761 acgctgcgtc acgagctcgg caaccagcgg atgctgggca tgtcactcga cgccggtggt
    20821 cacctgaccc acgggttccg ccccaacatc tccggcaaga tgttccacca gcgcagctac
    20881 ggcaccgatc ccgagactgg gctgctcgac tacgaccggg tggccgcggc ggcgcgcgag
    20941 ttcaggccgc tcatcctcat cgcgggctac tcggcgtacc cgcgtcggat caatttcgcg
    21001 aagatgcgcg agatcgccga cgaggtgggc gccaccctga tggtcgacat ggcgcacttc
    21061 gccgggctgg tcgccgggaa ggtgttcacc ggcgacgagg acccggtccc gcacgcgcac
    21121 gtcgtcacga cgaccacgca caagtcactg cgcggaccac gtggcggcct ggtgctggcg
    21181 acggcggagt actccgacgc ggtcgaccgg ggctgcccga tggtgctcgg cgggccgctc
    21241 tcgcatgtga tggcagccaa ggcggtggcg ctcgccgagg cgcgtcgtcc tgagttccag
    21301 gactacgccg cgcgcatcgc gaccaacgcg caggcgctgg ctgaggggtt cctgacgcgc
    21361 ggcgccaccc tggtcaccgg cggtacggac aaccacatcg tgctgctgga cgtgtcgtcg
    21421 ttcggcgtca ccggccggca ggccgagtcc gcactgctgg actcaggggt cgtgaccaac
    21481 cgcaactcgg tgccggccga cccgaacggt gcctggtaca ccagcgggat ccggctcggt
    21541 acgccggccc tcaccactcg cggcttcggt cccgacgagt tcgaccgagt ggccgagctg
    21601 atcgtcgagg tgctgacgaa cacctcggcc gatgccgcca agagcggtcc gtcgaaggcg
    21661 aagtacaccc tcgccgacgg cgtcgccgac cgggtcaagg ccgcctccgc cgagctgctc
    21721 gaccgctacc ccctctatcc cggcctcgag ctctgacggt ttgcgtcggc cggtcgacgg
    21781 ggttcagggg accccggtgt ggttaggagt ttgcgatgtt tacagcccaa aaggcgtgta
    21841 aacatcgcaa acctccgatg ggcacagcga gtctcggatg tggacatcgc aaatccaggc
    21901 tgatcagcca ccgatgtccg aaatccgcca gtcacaccga ccacaggggg acggcaagtc
    21961 ggccgaccag ctcctcaagc acggacgctc ccccgctcct cgctgccgac tcagcccagc
    22021 ccatcactac ggtgggccgg gcgatcacgc ccaccgatcg tgaccattcc cgccccgcag
    22081 ggtcctgaga accccgcagc tgcctggtta cccagccatc cggatgggta accaggcaaa
    22141 ccgccacggc ggtcagttgg cgatcgcgtc gctgatgatg atcgccacga gcgccaccgt
    22201 cgccagcg
//
LOCUS       DATBFZ010000497        10803 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_5135354, whole genome shotgun sequence.
ACCESSION   DATBFZ010000497 DATBFZ010000000
VERSION     DATBFZ010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 10803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10803
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5135354"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..925
                     /locus_tag="VH419_15170"
     CDS             77..925
                     /locus_tag="VH419_15170"
                     /inference="COORDINATES: protein motif:HMM:NF015743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017128 - phospholipid scramblase activity
                     [Evidence IEA];
                     GO_process: GO:0017121 - plasma membrane phospholipid
                     scrambling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid scramblase-related protein"
                     /protein_id="HEY7045009.1"
                     /translation="MTETAGYAPNWYPDPWGRHEHRYYDGRRWTEHVASHGRQSVDPP
                     GGAGHRPTVNRATEKVQRDVQRAGVQAGTSQGGGTLFTEPVLVVNQKAKLIEINQEFA
                     IYDQHGKQIGAVRQVGQSTAKKVMRFVADVDQFMTHKFQVVDSDGQVLMALTRPRKFM
                     KSRVMIEGGNGEEIGQIVQQNVIGKIRFALESDGQQVGSLNGENWRAWNFNIKDAAGR
                     EVARITKTWEGLAKTMFTMADNYVIQIHEQLPQPLHSIVLASAFGIDTALKQDARGLN
                     AGSLFG"
     gene            complement(1010..3049)
                     /locus_tag="VH419_15175"
     CDS             complement(1010..3049)
                     /locus_tag="VH419_15175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020576428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY7045010.1"
                     /translation="MDAEISHEQAHLLQSREALHRMRERTASMQAMSGDRVSGEYLKF
                     TLWKRMKALEDDPTIPLFFGRLDYGPAAATTVDSAGRPGERFYIGRRHVTDEQGDPLV
                     VDWRAPISRPFYRASKTTAMGVRLRRRFGFQHGQVTAYEDERLEAGGAPGLGDTAELH
                     SAILEAEIERPRTGPMRDIVATIQPEQDDIVRSELASTVCVQGAPGTGKTAVGLHRAA
                     YLLYAHRDQLNRQGVLVVGPNASFLRYIADVLPALGEIDARQTTVEELVAHVPVRSTD
                     PVQVARIKGDARMAVVLERALWSFVRAAPEGLVVPRGARRWRVPAYEIDEILAELRAR
                     GVRYGAGRGMLPQRLAHAVLVKMELAGDSPDDRVQNAVARSAPVKAMAEKLWPAVDAK
                     RLVWRLLSEPEFLASAADGLLTADEQQALWWPKPPKGPGSARWSLHDAVLIDEVSDLL
                     DRTPSLGHVVLDEAQDLSPMMLRSVGRQCSTGSATILGDLAQATTPWAVSAWDTALEH
                     VGKPTAHVEELTLGFRVPGDVIDYAAGLLPAIAPTLRRPTSVRRTRGELDIRVSQDLL
                     RDGVGAVRRLMDQEGSIGLIVADGSAARANAALTDADITHSVLGRDDDIESRVDVVPA
                     TLAKGLEFDHVVLLEPADIAADEADELTGLRRLYVCLTRAVTSLVVLHAKPIPRG"
     gene            complement(3205..4848)
                     /locus_tag="VH419_15180"
     CDS             complement(3205..4848)
                     /locus_tag="VH419_15180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020391871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair helicase XPB"
                     /protein_id="HEY7045011.1"
                     /translation="MTDGPLIVQSDKTLLLEVEHPKALECRRAIAAFAELERSPEHVH
                     TYRLTPLGLWNARAAGHDAEQVVDALLTYSRYPVPNSLLVDVAETMGRYGRLRLDKHP
                     THGLVLSSNDQNVFEEVLRAKRIAPMLGARIDEQIVVVHASDRGNLKQALLKLGWPAE
                     DFAGYVDGEAHPIALAEETWSLRPYQRDAADALWDGGSGVVVLPCGAGKTLVGAAAMA
                     KAQATTLILVTNTVAARQWKDELLRRTSLTADEIGEYSGAVKEIRPVTIATYQVMTTR
                     RKGVYSHLELFDARDWGLVIYDEVHLLPAPIFRLTADLQARRRVGLTATLIREDGREG
                     DVFSLIGPKRYDAPWKDIEAQGYIAPAECVEVRVDLTESERFVYATAEPEDKYRLAAA
                     CDSKRAVVDSLVEANRAEPTLVIGQYIDQLDELAEHLDAPLIKGETPVKERERLFAAF
                     RAGEVSLLVVSKVANFSVDLPEATVAIQVSGTFGSRQEEAQRLGRLLRPKADGRSARF
                     YSVVARDTVDADFAQHRQRFLAEQGYSYSIVDADHFLAH"
     gene            complement(4894..5424)
                     /locus_tag="VH419_15185"
     CDS             complement(4894..5424)
                     /locus_tag="VH419_15185"
                     /inference="COORDINATES: protein motif:HMM:NF018984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HEY7045012.1"
                     /translation="MDFVRYADFAAGLVNSPIESQHDVGDYLSGRQWLQPRIAERDVA
                     PLRRLRRELRPIFESSDTGDEAKVVELVNGLLTKYPVSPHIAGHDSQSWHLHVAERGS
                     SVAALITAECIMGLAMLVVDFGPTRLGVCSADRCDNVFVDTSPNKSRRYCSDRCSSRA
                     NVAAYRARRKAAATTG"
     gene            complement(5483..7207)
                     /locus_tag="VH419_15190"
     CDS             complement(5483..7207)
                     /locus_tag="VH419_15190"
                     /inference="COORDINATES: protein motif:HMM:NF025011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-associated domain-containing protein"
                     /protein_id="HEY7045013.1"
                     /translation="MSTSESVPRSLTQMLRERDDAVLVRLLLDRPDLAFPAPTDMSEV
                     ASRATTRHSVTEALALLNAFELWVATTVVARSAPFDIVDLSPGTDPTAVHRAVQRLVD
                     LGLVWGHADELTALRPVRAMSALLDQEGSPGPAPAADPPAVSSEHRTPALVDKLAAGS
                     AFELVQRMGVLLEHCGHQPVVLRRDGELTTRELRAFGSLLDVPSATTQVLFDLARRSG
                     LLGLGSDAGHEVLLPTRAFDDWLALSLADQWAVVVDAWFARHHESGPGWLKRLVLAAF
                     GEPGGGNAVPTADVLRWVAWHRPRRAPRSERVVTTMLDQAAWIGVTGIGAVASFALAA
                     DRSALAKLLPPLVDHVLIQADLTAVAPGPLLPEAAHDLGTLADVESRGGATVYRLSAE
                     SLTRAQGLGWSAAEILATLEKRSRTPLPQALEYLVHDLDRQRRARRGDRAVRAEVAIH
                     LEPERGVAPDVDELTVADRLDARLAEELVATLRGTDEPASHRDGDWDGGEPVFDSPLV
                     TLREAAETGEVVWVGYVDPLGVRSERLVRVGGVEDGLLRAKEAASGDEFSLAVHRITA
                     AHIIRASR"
     gene            7292..8404
                     /locus_tag="VH419_15195"
     CDS             7292..8404
                     /locus_tag="VH419_15195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020576055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY7045014.1"
                     /translation="MAGLPDDHEKGRGMALIQARQLSKSFGGKAAVRGLSFDVVPGSV
                     TGFLGPNGAGKSTTMRLMLGLDNGDGVTTYDGVTYRDLNEPLRHVGSVLDAKPFHPTR
                     TAINHLRMLAASNSIPDSRVTDVINIVGLGDVATAKPKTYSLGMGQRLGLACALLGDP
                     GTLILDEPANGLDPQGVHWLRNLMKMLAGQGRSVFVSSHLLSEMALMADHIVVIGRGS
                     LIASGRMSDFISTSRRNAVVARVDNPDLLRRELRARNAEAVAEQDGRLAITGSDCTTV
                     GGLAFDLGIRVFELFNREATLEEAFLDATGASEEFRASMGFAPHGAVSAYAGPGGPPG
                     PPGPPGLPGQQPGQPGPLGPPGQHGQYGQRPEGGGW"
     gene            8401..9231
                     /locus_tag="VH419_15200"
     CDS             8401..9231
                     /locus_tag="VH419_15200"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY7045015.1"
                     /translation="MISALKYEWRRITTIRSSYWMSGMAVALSAGVTMILILVVNEAN
                     SQGDLGDLAGVGVYTTWMVTAGGSSIAMPIVGAMFFAVMGAMAMGHEYRYGTNKATLT
                     AIPDRIAVLAAKAAVLSAWVAVAAAISLLLNAVLAWLFVGSTSIDTEALRPFLMYVLY
                     CVGFGLAGFGLSSIFRNQVGAIVTVLIWPLVLEPIIFAVLRATALANADIGLGKIANL
                     LPASAGRRTMFRPYELWSNLDTSDRAHIWSLAPSVLVFWCGIAALVVAGSVLFVKRDA
                     "
     gene            9357..>10803
                     /locus_tag="VH419_15205"
     CDS             9357..>10803
                     /locus_tag="VH419_15205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY7045016.1"
                     /translation="MGDRSQDSAETTLSYAAGPTDVPLLDETIGANFERTVAQYADNE
                     ALVEVATGRRWTYAELDRDVDALARGLVAAGVEIGDRVGVWSPNCAEWTMAQYATAKI
                     GAILVNINPAYRTHEVEYALNQSAVKLLISATTFKTSDYRAMIDDVRDQCTALQRVVY
                     IGTEDWSELVDAGKQSDADVVAARMAALAPDDAINIQYTSGTTGYPKGATLSHRNILN
                     NGYFVTELINFTSADRLAIPVPFYHCFGMVMGNLGCTSHGATMVIPAPAFDSEATLRA
                     ISDERCTGVYGVPTMFIAMQNHHAFADFDLSSLRTGLMAGSPCPVEVMKRCVKDMHMA
                     EVCIAYGMTETSPVSTQTRVDDDLERRTSTIGRVHPHVEIKITDPATGETLQRGEPGE
                     LCTRGYSVMLGYWQQDDKTAEAIDQDGWMHTGDLAVMREDGYCTIVGRIKDMVIRGGE
                     NIYPREIEEFLFRHPKVQDVQVFGVADAKYGE"
BASE COUNT         1721 a         3734 c         3691 g         1657 t
ORIGIN      
        1 cgccttgcca gatcgggcgc ggatgagccg ctcgcttcac cggcacccct tggaatcgat
       61 cgtctagagt tccgccatga ccgagacggc gggctacgca cccaactggt atccggaccc
      121 gtggggacgt catgagcacc gctactacga cggccggcgc tggaccgagc acgtcgccag
      181 ccacggccgc cagtcggtcg acccgcccgg cggtgcgggg caccggccga ccgtcaaccg
      241 ggcgaccgag aaggtccagc gggacgtgca gcgggccggc gtgcaggccg gtacgtcgca
      301 gggcggcggg acgctgttca ccgagccggt gctggtggtc aaccagaagg cgaagctgat
      361 cgagatcaac caggagttcg cgatctacga ccagcacggc aagcagatcg gagccgtccg
      421 ccaggtcggg cagagtaccg cgaagaaggt catgcgattc gtcgccgacg tcgaccagtt
      481 catgacgcac aagttccagg tcgttgactc cgacggtcag gtgctgatgg cgttgaccag
      541 accgcgcaag ttcatgaagt cgcgggtgat gatcgaaggc ggcaacggcg aagagatcgg
      601 tcagatcgtc cagcagaacg tcatcgggaa gatccgtttc gcgctggagt cggatggcca
      661 gcaggtcggc tccctgaacg gcgagaactg gcgcgcctgg aacttcaaca tcaaggacgc
      721 cgccggccgc gaggtcgccc gcatcacgaa gacgtgggag gggctggcga agacgatgtt
      781 caccatggcg gacaactacg tcatacagat ccacgaacag ctgccgcagc cgctgcacag
      841 catcgtgctc gcctccgcct tcggcatcga caccgccctg aagcaggacg cgcgcggcct
      901 caacgcggga tcgctcttcg gctgaccctc gcggcgaggt ggcaaagacg tttccggaga
      961 cggcgtgtct tgaaccaaac ttgccacctc ggcgagaagg ggcggtcgct cagccgcggg
     1021 ggatgggttt ggcgtgcagg acgacgaggg aggtgacggc tcgggtcagg cagacgtaga
     1081 gccgtcgtag cccggtcagc tcatctgcct cgtcggcggc gatgtcggcc ggttccagca
     1141 gcacgacgtg gtcgaactcc agacccttcg ccagggtcgc cggtacgacg tccaccctcg
     1201 actcgatgtc gtcatcgcgg cccagcacgc tgtgcgtgat gtcggcgtcg gtcagcgcag
     1261 cgttcgcccg cgccgccgag ccgtcggcca cgatcaggcc gatcgagccc tcctgatcca
     1321 tcagccggcg tacggcgccg acgccgtcgc gcagcaggtc ctgcgagacg cggatgtcca
     1381 gctcaccccg cgtacggcgc accgacgtcg gtcgtcgcag tgtcggcgcg atcgctggca
     1441 gcagtcccgc ggcgtagtcg atcacgtcac ccgggacccg gaagcccagc gtcagctcct
     1501 cgacgtgggc agtcggcttg ccgacatgct ccagggcggt gtcccaggcc gacacggccc
     1561 acggcgtcgt cgcctgcgcc aggtcgccga ggatcgtggc cgaccccgtc gagcactgcc
     1621 gtccgacgct gcgcagcatc atcggcgaca ggtcctgcgc ttcgtcgagc accacatgac
     1681 ccaggctcgg cgtccggtcc agcagatccg agacctcgtc gatcagcacg gcgtcgtgca
     1741 acgaccaacg cgcactgccc ggccccttgg gcggcttcgg ccaccacagc gcctgctgct
     1801 cgtcagcggt gaggaggccg tcggccgccg aggcgagaaa ctccggctcg gacagcagcc
     1861 gccacaccag ccgcttcgcg tccaccgccg gccacagctt ctctgccatc gccttcaccg
     1921 gggcactgcg cgccacggcg ttctgcacgc ggtcgtccgg tgagtcgccg gccagctcca
     1981 tcttcaccag caccgcgtgg gcgagccgct gcggcagcat gccgcgtccg gctccgtagc
     2041 ggacgccgcg agcacgcagc tcggcgagga tctcgtcgat ctcgtacgcc ggcacccgcc
     2101 accgccgggc gcctcgcggg accaccaacc cttccggcgc cgcgcggacg aacgaccaga
     2161 gcgcgcgttc gagcaccacc gccatccggg catcgccctt gatccgcgca acctggaccg
     2221 gatcggtcga ccggacgggg acatgggcga cgagctcttc gacggtggtc tgtcgcgcgt
     2281 cgatctcacc gagcgcgggc aggacgtcgg cgatgtagcg caggaacgac gcgttcggcc
     2341 cgaccaccag cacgccctgc cggttgagct ggtcacggtg ggcgtagagc aggtacgcgg
     2401 cccggtgcag cccgacagcc gtcttgccgg tgccgggcgc gccctggacg cacacggtcg
     2461 aagcgagctc cgaccggacg atgtcgtcct gctccggctg gatcgtggcc acgatgtcgc
     2521 gcatcggccc cgtacgcggc cgctcgatct ccgcctcgag gatcgcgctg tgcagttccg
     2581 ccgtgtcgcc aaggcccggc gcgccgccgg cctccaaccg ctcgtcctcg tatgccgtga
     2641 cctggccgtg ctggaaaccg aagcggcgac gcagccgcac ccccatcgcg gtcgtcttgc
     2701 tcgcccggta gaagggccgc gagatcgggg ctcgccagtc gaccacgagc gggtcgccct
     2761 gctcgtcggt cacgtggcgg cggccgatgt agaagcgctc cccgggccgg ccggcgctgt
     2821 cgaccgtcgt cgcggccgcc gggccgtagt cgagccgacc gaagaagagc ggaatcgtcg
     2881 ggtcgtcctc gagcgccttc atccgcttcc agagggtgaa cttcaggtac tcaccgctga
     2941 cgcggtcccc gctcatggcc tgcatcgacg cggtgcgttc acgcatccgg tggagcgcct
     3001 ctcgcgactg gagcagatga gcctgttcgt gggagatctc ggcgtccact ggccggagaa
     3061 tagcaagttg gccgaggtcg cctcaccggg ttttacaggt acccggctgc cgggttggcc
     3121 gcgaattgcg ggaaagttgc ggtccacagg ccggtctgga cctgacgttt cccgcattcc
     3181 gcgggaacca gtgacgtacg gcgctcagtg ggccaggaag tggtcggcgt cgacgatcga
     3241 gtacgagtag ccctgctcgg cgaggaagcg ctgccggtgc tgggcgaagt cggcgtcgac
     3301 cgtgtcccgc gcgaccacgc tgtagaaccg ggcggaccgg ccgtcggcct tgggacggag
     3361 caggcgaccc aaccgctgcg cctcctcctg gcgtgatccg aacgttcccg agacctggat
     3421 ggccacggtc gcctccggca ggtcgacgga gaagttggcg accttgctga cgacgagcag
     3481 cgacacctcg ccggcccgga acgccgcgaa cagccgctcc cgctccttca ccggtgtctc
     3541 acccttgatg agcggcgcgt ccaggtgctc ggcgagctcg tcgagctggt cgatgtactg
     3601 gccgatcacc agagtgggtt cggcccggtt cgcctccacc agcgagtcca cgaccgcgcg
     3661 cttgctgtca cacgccgccg ccagccggta cttgtcctcg ggctcggcgg tcgcgtagac
     3721 gaaccgctcg ctttcggtca ggtcgacccg cacctcgacg cactcggccg gagcgatgta
     3781 accctgcgcc tcgatgtcct tccacggggc gtcgtaccgt ttcggtccga tcagagagaa
     3841 cacgtcaccc tcacggccgt cctcacggat gagcgtggcg gtcagtccca cccgccgccg
     3901 cgcctgcaga tctgccgtga gccggaagat cggtgccggc agcagatgca cctcgtcgta
     3961 gatcaccagc ccccagtcgc gggcgtcgaa cagctcgagg tgtgagtaca cgcccttgcg
     4021 acgcgtcgtc atcacctggt acgtcgcgat cgtcaccggg cggatctcct tgacggcgcc
     4081 ggagtactca ccgatctcgt ctgccgtcaa cgacgtacga cgcaacagct cgtccttcca
     4141 ctgccgggct gcaaccgtgt tggtgacgag gatgagcgtc gtcgcctgcg ccttggccat
     4201 ggcggctgca ccgaccagtg tcttcccggc gccgcacgga agtacgacga ctccggagcc
     4261 gccgtcccac aaggcgtcgg cggcatcacg ttggtacggc cgcagcgacc aggtctcctc
     4321 ggcgagcgcg atcggatggg cctcgccgtc gacgtagccc gcgaagtcct cggccggcca
     4381 gcccagcttg agcagcgcct gcttgaggtt tccgcggtcg gatgcgtgga cgacgacgat
     4441 ctgctcgtcg atccgcgcgc cgagcatcgg tgcgatgcgt ttggcgcgca gcacctcctc
     4501 gaagacgttc tggtcgttac tcgagaggac gagtccgtgg gtcgggtgct tgtcgaggcg
     4561 cagccgcccg taccgcccca tcgtctcggc gacgtcgacg agcagcgagt tcggcacggg
     4621 gtagcgcgag tacgtgagca gcgcgtcgac gacctgctct gcgtcgtggc cggcggcccg
     4681 ggcattccac agacccaggg gcgtgaggcg gtaggtgtgc acgtgctccg gcgagcgctc
     4741 gagctcggcg aacgccgcga tcgcccggcg gcactccagc gccttcgggt gctcgacctc
     4801 gagcagcaag gtcttgtcgg actgaacgat cagcggaccg tcggtcattc gcgtggtact
     4861 cctgggagtc ggtcggtgca gcggggaggg agctcagccg gtggtggcag cggccttgcg
     4921 acgcgcacgg tacgccgcga cgttggcccg tgaggagcac cggtccgagc agtaccgccg
     4981 ggacttgttc ggcgacgtgt cgacgaagac gttgtcgcag cggtcggccg agcagacgcc
     5041 cagccgggtg gggccgaagt cgacgacgag catggccagt cccatgatgc actcggccgt
     5101 gatgagcgca gccacgctcg accctcgctc ggcgacgtgc agatgccacg actgcgagtc
     5161 gtgcccggcg atgtgcggtg acaccgggta cttggtgagc aggccgttca ccagctcgac
     5221 caccttcgcc tcgtctccgg tgtcggacga ctcgaagatc ggccgcagct cacgtcgcag
     5281 ccggcgcagc ggtgccacgt cgcgctcggc gatgcgcggc tgcagccact ggcggccgct
     5341 caggtagtcg ccgacgtcgt gctggctctc gatcggggag ttgaccagcc cggccgcgaa
     5401 gtcggcgtac cgaacgaagt ccacgacccc atggtaggcc gttcacgcca ggatgggtaa
     5461 tgcccaagat gtcccgcagg tattaccgag aggcgcggat gatgtgggcg gcggtgatgc
     5521 ggtgcacagc cagtgagaac tcgtctcccg acgccgcttc cttggcccga agcaggccgt
     5581 cctcgacccc gccgacccgg acaagtcgtt cactgcgtac gccgagtggg tcgacgtacc
     5641 ccacccagac cacctcgccg gtctccgccg cctcccgcaa ggtgacaagt ggtgagtcga
     5701 agacgggttc gccaccgtcc cagtccccgt cacggtgcga cgccggctcg tcggtgccac
     5761 gcaacgtcgc gaccagctcc tcggccagcc tcgcgtcgag tcggtcggcc accgtgagct
     5821 cgtcgacgtc cggggcgacc ccccgctccg gctcgaggtg gatggccacc tcggcgcgga
     5881 cggcgcggtc gcctcgccga gcccggcgct gccggtcgag gtcgtggacg aggtactcca
     5941 aggcctgcgg cagcggtgtg cgagaccgct tctcgagggt ggccaggatc tctgccgcgc
     6001 tccagccgag gccctgtgcc cgcgtcagcg actctgcgct caaccggtac acggttgcgc
     6061 cgccgcgaga ctcgacgtcg gcgagtgtcc cgaggtcgtg ggccgcctcc ggtagcagcg
     6121 gtccgggcgc caccgccgtc agatcggcct ggatcaagac gtggtcgacg agcggaggca
     6181 gtagcttggc cagcgcactc cggtccgccg cgagcgcgaa cgacgcgacc gcgccgatac
     6241 cggtgacgcc gatccaggca gcctggtcca gcatcgtcgt gacgacccgc tccgaacgcg
     6301 gcgctcgacg tggtcggtgc caggcgaccc agcggagcac gtcagccgtc ggtacggcgt
     6361 tcccgccgcc cggctcgccg aaggccgcga ggacaagacg cttgagccaa cccgggccgc
     6421 tctcgtggtg tcgcgcgaac cacgcgtcca cgaccacggc ccactggtcg gcaagcgaca
     6481 gtgccagcca gtcgtcgaat gcccgggtcg gcagcagcac ctcgtggccg gcatcgctgc
     6541 caagcccgag cagtccggac cggcgggcca ggtcgaacag aacctgagtc gtggcggacg
     6601 gcacgtccag caggctccca aaggcccgta gctcgcgagt cgtgagctca ccgtcacggc
     6661 gcagcaccac cggctggtgg ccgcagtgct cgagcagcac acccatgcgc tggacgagct
     6721 cgaaggccga gccggcggcg agcttgtcca ccagggccgg cgtacggtgc tcggaactga
     6781 cggccggcgg atcggctgcg ggcgccggcc cgggactgcc ctcctgatcg agcagcgccg
     6841 acatcgcccg taccgggcgt agcgcggtca gctcgtcggc gtggccccag acaagcccca
     6901 ggtcgaccag tcgttgcacc gcgcggtgga cggcggtcgg gtctgtgccc ggcgagaggt
     6961 cgacgatgtc gaacggcgcg gagcgagcga ccaccgtggt cgctacccac agctcgaacg
     7021 cgttcaacag ggccagcgcc tccgtcaccg agtggcgggt ggtcgcgcgc gacgccacct
     7081 cgctcatgtc ggtcggagcc gggaacgcga ggtcgggccg gtcgagcagg agccgcacca
     7141 gcaccgcgtc gtcgcgttca cgcagcatct gcgtgaggct cctcggcacc gactcgctcg
     7201 tgctcatcat gtccacgtta cgcgcgccgc cggaggtggt gcggattcgt gccgatgggt
     7261 gccgggaacc ggacgatatc ggcctgtcct cgtggcagga ttgcccgacg accacgagaa
     7321 gggacgagga atggcgctca tccaggcgcg gcagctgagc aagagcttcg gcggcaaggc
     7381 tgccgttcgc ggcctgtcgt tcgacgtcgt acccggctcc gtcacgggct tcctcggacc
     7441 caacggtgcc ggcaagtcga ccaccatgcg gctcatgctc ggcctggaca acggcgacgg
     7501 cgtgacgacg tacgacggtg tcacctaccg cgacctgaac gagccactgc ggcacgtggg
     7561 gtcggtcctg gacgcgaagc cgttccatcc gacgcgtacg gcgatcaacc acctgcggat
     7621 gttggccgcc tcgaactcga tccccgactc gcgggtcacg gacgtgatca acatcgtggg
     7681 tctcggggac gtggcgacgg cgaagcccaa gacgtactcg ctcggtatgg gacagcggct
     7741 cgggctcgcc tgcgcgctgc tcggcgaccc aggcacgctc atcctcgacg agccggccaa
     7801 cggtctcgac ccacaaggcg tgcactggct tcgcaacctg atgaagatgc ttgccggtca
     7861 gggccggtcg gtcttcgtct ccagccacct gctgtccgag atggcgttga tggccgacca
     7921 catcgtcgtg atcggccgcg gttcgctgat cgccagcggc aggatgagcg acttcatctc
     7981 gacgagccgg cgcaacgccg tcgtcgcccg tgtcgacaac cccgacctgc tcagacgtga
     8041 gctgcgggca cgcaacgccg aggcggtggc agaacaggac ggccgactcg ccatcaccgg
     8101 ctcggactgc acgaccgtcg gtgggctggc gttcgacctg ggcatacgcg tcttcgagct
     8161 cttcaaccgt gaggcgaccc ttgaagaggc gttcctcgac gccaccggtg cgtcggagga
     8221 gttccgggca tcgatgggct tcgcgccgca cggcgccgtt tcggcgtacg ccggtccagg
     8281 tgggccgccc ggcccacccg gcccacccgg cctaccgggg cagcaaccgg ggcagcctgg
     8341 tccgctcggt ccgcctggac agcatggtca gtatggtcag cggccggaag gaggtggctg
     8401 gtgatcagcg cgctgaagta cgagtggcgg cggatcacga cgatccggtc gagctactgg
     8461 atgagcggga tggccgtcgc cttgtcggcc ggcgtcacga tgatcctcat cctcgtcgtc
     8521 aacgaggcga actcccaggg cgacctcggt gaccttgccg gtgtcggcgt ctacacgacg
     8581 tggatggtga ccgccggagg ttcgtcgatc gcgatgccga tcgtcggcgc gatgttcttc
     8641 gccgtgatgg gtgccatggc gatgggccac gagtaccgct acggcaccaa caaggcgacg
     8701 ctgaccgcga tcccggaccg gattgccgtg ctcgcggcca aggcggccgt gctgtcggcg
     8761 tgggtcgccg tcgccgccgc catcagcctg ctgctcaatg ccgtgctggc ctggctgttc
     8821 gtcgggtcga cgtcgatcga caccgaagcg ctgcgcccgt tcctcatgta cgtcctgtac
     8881 tgcgtcggct tcggtctcgc cggcttcgga ctctcgtcga tcttccgcaa ccaggtcggc
     8941 gcgatcgtca ccgtcttgat ctggccgctc gtgctcgagc cgatcatctt cgccgtgctt
     9001 cgcgcgacag cgctggccaa cgccgacatc ggcctcggca agatcgccaa cctgctgcct
     9061 gcgtcggccg gtcggcggac gatgttccgg ccgtacgagc tctggtccaa cctcgacacc
     9121 tccgatcgag cgcacatctg gtccctcgcc cccagcgtgc tcgtcttctg gtgtggcatc
     9181 gccgccctcg tggtcgccgg ttcggtcctc ttcgtcaagc gcgacgcctg accgctggcc
     9241 ttcccgcgaa ttgcgggaag ttccggaaat tcgggagcga gaaagccgga actcgccgca
     9301 attcgcgggg aatctgggga tggttggagg gatgcggacg gtcttaggct gcgggcatgg
     9361 gtgaccgttc gcaggactcc gccgagacaa cgctctcgta cgccgccgga ccgacagacg
     9421 tacctctcct ggacgagacg atcggcgcca acttcgagcg tacggtcgcc cagtacgccg
     9481 acaacgaggc gctcgtcgag gtcgccactg gtaggcggtg gacgtacgcc gagctggacc
     9541 gcgacgtcga cgcgctggcg agaggactgg tcgcggcggg tgtcgagatc ggcgaccgcg
     9601 tcggggtgtg gtcgccgaac tgcgccgagt ggaccatggc gcagtacgcg accgccaaga
     9661 tcggcgccat cctcgtcaac atcaacccgg cgtaccggac gcacgaggtc gagtacgcgc
     9721 tcaaccagtc cgcggtgaag ctgctcatct cggcgacgac gttcaagacc agcgactacc
     9781 gggcgatgat cgacgacgtc cgcgatcagt gcacggcact ccagcgggtc gtctacatcg
     9841 gtaccgagga ctggtcggag ctcgtcgacg cgggcaagca gtcggacgcc gacgtggtcg
     9901 ccgcgcggat ggcggcactg gcacccgacg acgcgatcaa catccagtac acgtcgggta
     9961 cgacgggcta cccgaagggc gcgacgctca gccaccgcaa catcctgaac aacggctact
    10021 tcgtgaccga gctcatcaac ttcacatccg ccgaccgcct cgcgatcccg gtgccgttct
    10081 accactgctt cggcatggtg atgggcaacc tcggctgtac gagccacggc gccacgatgg
    10141 tgatcccggc tcccgcgttc gactcggaag ccacgctgcg cgcgatcagc gacgagcggt
    10201 gcacgggtgt gtacggggtg ccgacgatgt tcatcgcgat gcagaaccac cacgccttcg
    10261 cggacttcga cctgtcatcg ctccgcaccg ggttgatggc gggatcgccc tgcccggtcg
    10321 aggtgatgaa gcgctgcgtc aaggacatgc acatggcgga ggtctgcatc gcctacggca
    10381 tgaccgagac gtcgccggta tcgacccaga cgcgcgtgga cgacgacctc gagcggcgta
    10441 cgtccacgat cggacgggtg catccgcacg tcgagatcaa gatcaccgac ccggccaccg
    10501 gtgagaccct gcagcgcggc gagcccggcg agctgtgcac ccggggatac tccgtgatgc
    10561 tcggctactg gcagcaggac gacaagacgg cggaggcgat cgaccaggac ggctggatgc
    10621 acaccggcga tctcgccgtc atgcgcgagg acggctactg caccatcgtc ggacgcatca
    10681 aggacatggt gatccgcggc ggcgagaaca tctatccgcg cgagatcgaa gaattcctat
    10741 tccggcatcc gaaggtgcag gacgtgcagg tcttcggcgt cgccgacgcg aagtatggcg
    10801 agg
//
LOCUS       DATBFZ010000498         1803 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_4478168, whole genome shotgun sequence.
ACCESSION   DATBFZ010000498 DATBFZ010000000
VERSION     DATBFZ010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1803
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4478168"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            807..1085
                     /locus_tag="VH419_15210"
     CDS             807..1085
                     /locus_tag="VH419_15210"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEY7045017.1"
                     /translation="MTRPGNRIELIRNAEPTVLRLVGELRSADAPYIDAMLKVIHDTE
                     PREVVLDLTGAVFLDTCMLKSIADARKSGVRLTIQWPPSDPRPGHADA"
     gene            1126..1701
                     /locus_tag="VH419_15215"
     CDS             1126..1701
                     /locus_tag="VH419_15215"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEY7045018.1"
                     /translation="MAELLVDFITSLDGYGAAEGWPGWWGLEGPEYLTWLSEVPEGDY
                     VVLMGATTYRIMSGFAAQSEPGTGSLTDMSKVVFSSTLTEPLSWANTQLVARDAVEVV
                     REMKNERSTSMRTIGSITLCRSLLEAGLVDRFRVVVFPVITGRTGRDRIYDGYPDVGL
                     EMISSRTFDGRTQLLEYVPTVLAGPPVRNSR"
BASE COUNT          318 a          569 c          611 g          305 t
ORIGIN      
        1 cgccgccagg cgcgacgccg acgacgtcca gcgtcgtagg acctgggtcc gccggcacgc
       61 ctcctacgtc atcatcgaca gggccgacga ccgtcggcgc cggtgccaca gacaatccct
      121 ccgttgctcc acctggctcc gccggtggcg gcgacctgtc cgcgccgagc aacgaggtgg
      181 cagaagcggc cgctccgcca gaacaacagt ccgctccgct cgagcgccaa cgacaacgaa
      241 caacaggtac cgcggccgcg gcgatcggtg tcatgaccgt cgcgggattc gccctcggcc
      301 tccatgagcg gcgggtgaca agacgcaagc ccacggcgtg acatcgcaga ctgagttctc
      361 agccgctgaa gatgttcgct gtcggtttga acaccggatc atccggcact ctgactcgta
      421 ggagcgactc gccgaaatct gctgaggact ccgctcctgg aggtcagcga cgcggagtcc
      481 tcagcgggcg agcgccggtt tgccaccggg cctgccgaag accccgcctc attgcggggc
      541 cttcgggcaa gacagcctca accagcagga tggatcgggt ctgtctccga tagagatgcc
      601 cgatccgacc gtgttcaaga ccgtgccgtg aggcgttgag atgtcgacag tgaacgagcg
      661 catgtgcacc gtgtttgcgg gccctggagg atggagcccg ggtcgtccag ttggcaccga
      721 gctcggccga cgatggtctt cgacgcgctg acaacagacg cttggccgag gtaggtcgcg
      781 aaccggcggt gggtaggcgt ctgcgtatga ctcgccccgg caaccgcatc gagctgatcc
      841 ggaacgccga gccgacggtg ctcaggctcg tcggcgaact ccggtcggcc gacgcgccat
      901 acatcgacgc catgttgaaa gtgatccacg acactgagcc acgggaggtc gttctcgacc
      961 tcaccggcgc agtgttcctc gacacatgca tgctcaagtc catcgccgac gctcggaagt
     1021 ccggcgtccg cctcaccatt cagtggccac cgtcagatcc gcgcccgggt catgccgacg
     1081 cgtgacgctg gttgagatgc gatgagagcg gaaaggcgat gaaccatggc agagctgctg
     1141 gtcgacttca tcacgtcgct cgacgggtac ggggccgccg aggggtggcc cggttggtgg
     1201 ggcctggaag gccctgagta cctgacgtgg ttgagcgaag tgcctgaagg cgactacgtg
     1261 gtgctgatgg gcgcgaccac gtaccgcatc atgtcgggat tcgcggcgca gagtgagccg
     1321 ggcaccgggt cgctgacgga catgtcaaag gtggtgttct cgtccacgtt gacggagcca
     1381 ctctcgtggg cgaacacgca gctcgtggct cgagatgcgg ttgaggtggt gcgcgagatg
     1441 aagaacgagc gctccacgtc gatgcggacc atcggcagca tcacgctgtg ccgctcactg
     1501 ctcgaagctg gcctcgtgga tcgctttcgc gtcgttgtct ttccggtcat caccggtcgc
     1561 actggacggg accggatcta cgacggctac ccggatgtcg gcctggagat gatcagtagc
     1621 cgcaccttcg atgggcgaac ccagctgttg gagtacgtgc cgacggtcct ggccgggccg
     1681 cctgtgagga acagccgctg accgttcctc gccagagacg aagaggtgcc gtggtcgtcg
     1741 ccgacgacgt catggatgtt ggagatcgga tcggagggct gctcatcgac agcgtggtgc
     1801 gcc
//
LOCUS       DATBFZ010000499         4600 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_6123212, whole genome shotgun sequence.
ACCESSION   DATBFZ010000499 DATBFZ010000000
VERSION     DATBFZ010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 4600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4600
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6123212"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="VH419_15220"
     CDS             <1..321
                     /locus_tag="VH419_15220"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY7045019.1"
                     /translation="DDALQGYPDAGTLPARLQRLQERIRQGRDYELTAAELRLIHFLP
                     THLSLQEIADRLYLSRPTVKTHIASIYSKLGVQGRSQAVELINQTGLGSTENAATVPD
                     PDRD"
     gene            445..1056
                     /locus_tag="VH419_15225"
     CDS             445..1056
                     /locus_tag="VH419_15225"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEY7045020.1"
                     /translation="MNIPINAKVECTDGACGRSTNVVVNPVNHTVTHVAIEDKTLPDN
                     PSRLVPVANVSEVADGRITLSCTRADVAKLGPFIVADLVQQSPSGGAYSYGEGYSAQY
                     VLDDTAYDEVEEEEIPEGERALYGGMHVKASDGRVGKLDELVLDSESGMITGLVMRKG
                     HLWGSRDVTVRVDQIESVDGDTVYLKLDKSAVGALPAMKVRRG"
     gene            1170..1742
                     /locus_tag="VH419_15230"
     CDS             1170..1742
                     /locus_tag="VH419_15230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7045021.1"
                     /translation="MVDKSAGRRKRRTAAPLLALVGILVLVAAGCGGKSADQKASEAY
                     ANSVCTAIAGWQTQVKSITTDLSGGISRASLQSKVTRVESATKSLVTQIKTIPLPNTS
                     QGQTTRQQLDQLSANLTSTVASIKAAFAGLQTDASAGTVAAAVLTLAPQVKTLATSAQ
                     STISTLKSSEEARSSAFKNAESCQSLGGSG"
     gene            1748..2347
                     /locus_tag="VH419_15235"
     CDS             1748..2347
                     /locus_tag="VH419_15235"
                     /inference="COORDINATES: protein motif:HMM:NF015674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEY7045022.1"
                     /translation="MTTVAQEQVYAENAAPVWWVFLITGTAWLVLSLIMFRFDISSAK
                     TIGVLAGIVFLAAGVIEFCVAAVVTSGWWKALNAVLGVLLVVGGIMSFIHPGNAFVAV
                     ASIIGFMFLLVGIFDLIVAFSDRTGLWWVRMITGFICIGLAFWASGDFDRKSILLVAW
                     LGLFALLRGINSFIVAFTLHHLEKDAAADRLAVAAIEGR"
     gene            2350..>4600
                     /locus_tag="VH419_15240"
     CDS             2350..>4600
                     /locus_tag="VH419_15240"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HEY7045023.1"
                     /translation="MSKPFKGVINIDIKDSTPDWTPYAQPVPPAGAPNVVYLVLDDVG
                     FSALEPYGGPIETPNIKRVADKGLKYTNFHTTALCSPTRSCFLTGRNHTTNGMACISE
                     ASSGFPNASGHIPLECAMAPEILGEQGWSTFMVGKWHLCAEDEENLASRRLGWPSGRG
                     FDRWYGFIGAETNQWYPDLIHDNHTVDPPKTPEEGYHLSIDLTDKALSFVRDMKAVAP
                     DKPFFIYYAFGAGHAPHHVPKEWADKYKGKFDMGYEKIRETILENQKKLGIVGKDVEL
                     SPINPYTDLKGPKGQDWPALDVVKPWDSLTDDEKKLFSRMAEVYAGFVSHADHEFGRF
                     LDYLEESGELDNTLIIIVSDNGASGEGGPNGSVNENKFFNGIDDTMEDNLKYLDVLGS
                     TKTYNHYPTGWAWALNTPNKMWKRYSNYQGGTADPMIVSWPAKIKKAGIRTQYQHAID
                     IVPTIYDCLGVEMPEVYRGATQIPLEGASFKATFDDEKAPGRETQFYSMLSTRGIYHK
                     GWKAASVTPATPDGWADFTNQRWELFNTETDPSEIHDLADKELEKLNELISLWWSEAG
                     KYNALPLESRTAVDILTTERPQLTKPRNQYTYYPNCAEVPEAAAVNVRNRSYAIGADV
                     TIDKDPAGVLFAHGCRFGGHALYVKDGKLKYDYNYVGMEDQYVESSKPVPTGDCRLAA
                     VFEKDDSKGMPTTGTLSLYINKEKVGEAKIKTQPGKFSLAGEGLNIGRDGGEPVTTDY
                     PGDAPWAFTG"
BASE COUNT          886 a         1550 c         1464 g          700 t
ORIGIN      
        1 gacgacgcct tgcagggcta ccccgacgcc ggaacgctgc cggcccggct ccagcgcctc
       61 caggaacgca tcaggcaggg aagagactac gagctcaccg ccgccgaact gcgactgatc
      121 cacttcctcc cgacccacct ctccctgcag gagatcgccg accgcctcta cctctcacgc
      181 ccgaccgtca agacccacat cgcctcgatc tacagcaagc tcggcgtcca gggcagatcc
      241 caagccgtcg agctcatcaa ccagaccggc ctcggctcga ccgagaacgc ggccacggtt
      301 cctgatccag atcgcgactg aggccgcgag ccgcctcgcg acgaggccgg gcgatctccg
      361 cctttttggc cgatgacgcg gcgggcgtcc gttcgtagcg ttgaggtcgc ctgcacggca
      421 gtcggcgaaa ggaggctggt cgcagtgaac atcccgatca acgcgaaggt cgaatgcacg
      481 gacggcgcct gcgggaggtc gacgaacgtc gtcgtcaacc cggtcaacca cacggtgacg
      541 cacgtggcga tcgaggacaa gaccctcccc gacaacccgt cgcgcctcgt gccggtagcc
      601 aacgtgagcg aagtcgccga cggccgcatc acgctgagct gcacgagggc ggacgtcgcg
      661 aagctgggcc cgttcatcgt cgctgacttg gtccagcagt caccgtccgg tggggcgtac
      721 tcgtatggcg agggttacag cgcccagtac gtcctcgacg acaccgcgta cgacgaggtc
      781 gaggaagagg agatccccga gggagagcgt gccctctacg gcggcatgca cgtgaaggcg
      841 tcggacggcc gggtcggcaa gctcgacgag ctcgtgctcg actcggagag cggcatgatc
      901 accggtctcg tgatgcgcaa ggggcacctc tggggctcga gggacgtcac cgtccgcgtc
      961 gaccagatcg agtcggtcga cggcgacacc gtgtacctga agctcgacaa gtcggcggtc
     1021 ggagcgctgc cggcgatgaa ggtgcggcgg ggctaggccc cccaatctca gccatttcgg
     1081 ccgatgacgc aacgacagtc gcggtcgtat cgtcgactca ctgagggagg ccgcgggctt
     1141 cccgtgagat ccggcggagg aggttctcga tggtcgacaa gtcagccgga cgtcggaagc
     1201 gccgcaccgc tgcgccgctt ctggctctcg tcggaatcct cgtcctggtg gcggccggat
     1261 gcggaggcaa gagcgcagac cagaaggcga gcgaggcgta cgcgaacagc gtttgcactg
     1321 cgatcgcggg ctggcagacg caggtcaaga gcatcacgac cgacctcagc ggcgggatct
     1381 cccgggcgtc gctccagtcg aaggtgacgc gggtcgagtc cgcaaccaag agcctcgtga
     1441 cccagatcaa gacgattccg cttccgaaca catcacaagg ccagacaacc aggcagcagc
     1501 tcgatcagct ctcggccaac ctcacgtcga ccgtcgcctc gatcaaggct gccttcgccg
     1561 ggcttcaaac cgacgcctcg gccggcacgg tcgccgccgc ggtgctcacg ctcgcgcccc
     1621 aggtgaagac cctcgcgacc tcggcccagt cgacgatttc gacgctgaag agctccgagg
     1681 aagcgcgctc gtcggccttc aagaacgccg agtcctgcca gagcctcgga ggcagcggct
     1741 gatgtccatg accaccgtcg cccaggaaca ggtctacgcg gagaacgccg ccccggtgtg
     1801 gtgggtgttc ctgatcaccg gcaccgcctg gctcgttctc tcgctgatca tgttccggtt
     1861 cgacatctcc tccgcgaaga cgatcggcgt gctcgccgga atcgtgttcc tggccgcagg
     1921 cgtgatcgag ttctgcgtgg cggccgtcgt cacgagcggc tggtggaaag ccctgaacgc
     1981 ggtcctcggc gtgctgctcg tcgtcggcgg gatcatgtcg ttcatccatc cggggaacgc
     2041 gttcgtcgcg gtcgcgtcga tcatcggatt catgttcctc ctggtcggca tcttcgacct
     2101 gatcgtcgcg ttctccgatc gcaccggcct ctggtgggtg cgaatgatca ccggcttcat
     2161 ctgcatcggc ctcgcgttct gggcctccgg cgacttcgat cgcaagtcga tcctgctcgt
     2221 cgcctggctc ggcctgttcg cgctgctccg cggaatcaac agcttcatcg tcgcattcac
     2281 tttgcaccac ctagaaaagg acgccgcggc cgatcggctt gcggtcgccg cgatcgaagg
     2341 gagataacca tgagcaagcc attcaagggt gtcatcaaca tcgacatcaa ggactcgacg
     2401 ccggactgga cgccgtacgc ccagcccgtg ccgccggcgg gcgcaccgaa cgtcgtgtat
     2461 ctcgtgctcg acgacgtcgg cttctcggcg ctcgagccgt acggcgggcc gatcgagacg
     2521 ccgaacatca agcgcgtcgc cgacaagggc ctgaagtaca cgaacttcca cacgacggcg
     2581 ctctgctcgc cgacgcgttc gtgcttcctc accggccgca accatacgac gaacggcatg
     2641 gcctgcatct ccgaggcgtc ctcgggcttc ccgaacgcga gcggacacat cccgctcgag
     2701 tgcgcgatgg cccccgagat cctgggcgag cagggctgga gcacgttcat ggtcggcaag
     2761 tggcacctgt gcgccgagga cgaggagaac ctcgcctcca gaaggctcgg gtggccgtcc
     2821 ggccgcggct tcgaccgctg gtacggattc atcggcgccg agaccaacca gtggtatccg
     2881 gacctgatcc acgacaacca cacggtcgac ccgccgaaga cgccggaaga gggctatcac
     2941 ctctcgatcg acctcaccga caaggcgctg tcgttcgtcc gcgacatgaa ggccgtcgcg
     3001 ccggacaagc cgttcttcat ctactacgcc ttcggcgccg gccacgcgcc gcatcacgtc
     3061 ccgaaggagt gggcggacaa gtacaagggc aagttcgaca tgggctacga gaagatccgc
     3121 gagacgatcc tcgagaacca gaagaagctg gggatcgtcg gcaaggacgt cgagctctcg
     3181 ccgatcaacc cgtacaccga tctcaagggg cccaagggac aggactggcc ggcactcgat
     3241 gtcgtcaagc cgtgggactc gctcaccgac gacgagaaga agctgttctc ccgcatggcc
     3301 gaggtctacg cgggcttcgt cagccacgcc gaccacgagt tcggccgctt cctcgactac
     3361 ctcgaggagt caggggagct cgacaacacc ctgatcatca tcgtgtcgga caacggcgcg
     3421 tccggtgagg gcggcccgaa cggctcggtc aacgagaaca agttcttcaa cggcatcgac
     3481 gacacgatgg aggacaacct caagtacctc gacgtgctcg gcagcacgaa gacctacaac
     3541 cactatccga ccggctgggc gtgggcgctc aacacgccga acaagatgtg gaagcggtac
     3601 tcgaactacc agggcggcac ggccgatccg atgatcgtct cgtggcccgc caagatcaag
     3661 aaggcgggca ttcggacgca gtaccagcat gcgatcgaca tcgtgccgac gatctacgac
     3721 tgcctcggtg tcgagatgcc cgaggtgtac aggggtgcca cacagatccc gctcgaaggc
     3781 gcgagcttca aggcgacctt cgacgacgag aaggcgccgg gcagggagac gcagttctac
     3841 tccatgctca gcacgcgcgg catctaccac aagggctgga aggccgcgtc ggtgacgccg
     3901 gccaccccgg acggctgggc ggacttcacc aaccagcgct gggagctgtt caacaccgag
     3961 accgatccga gcgagatcca cgacctcgcc gacaaggagc tggagaagct gaacgagctg
     4021 atctcgctct ggtggtcgga ggcgggcaag tacaacgccc tcccgctcga gtcgcggacc
     4081 gccgtggaca ttctcaccac cgagcggccg cagctcacga agccgcgcaa ccagtacacc
     4141 tactacccga actgcgccga ggtccccgag gcggccgcgg tgaacgtccg caaccgctcc
     4201 tatgcgatcg gggcggacgt cacgatcgac aaggaccccg caggcgtgct gttcgcgcac
     4261 gggtgtcgct tcggcggcca cgccctgtac gtcaaggacg ggaagctcaa gtacgactac
     4321 aactacgtcg gcatggagga tcagtacgtc gagtcctcca agcccgtccc gaccggcgac
     4381 tgcaggctcg ccgccgtgtt cgagaaggac gacagcaagg ggatgccgac caccggcacg
     4441 ctgagcctct acatcaacaa ggagaaggtc ggagaggcga agatcaagac ccagcccggc
     4501 aagttctcgc ttgccggcga gggcttgaac atcggccgtg acggcggtga gccggtcact
     4561 accgactacc cgggagacgc cccgtgggcg ttcaccggcg
//
LOCUS       DATBFZ010000500        15955 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_5766678, whole genome shotgun sequence.
ACCESSION   DATBFZ010000500 DATBFZ010000000
VERSION     DATBFZ010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 15955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15955
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5766678"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2736)
                     /locus_tag="VH419_15245"
     CDS             complement(<1..2736)
                     /locus_tag="VH419_15245"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY7045024.1"
                     /translation="MPVCPGCGETNPPRFRLCGFCGTPLGAPVREVRKTVSIVFCDLK
                     GSTSLGEHLDSEALREVMTRYFDEMRVVLEQHGGTVEKFIGDAVMAVFGLPRAHEDDA
                     LRAVRAAVDMRKSLLRLNEELMRRWGVRMANRTGVNTGEVVAGDPAAGQRLVTGDAVN
                     VAARLEQAATENGILIGSDTYRLVKEAVTVERLDPLPLKGKAEPVPAYLLLGVDPGAS
                     AVPRRLDTPMVGRQQELQLLRERFDEAVTGRRCQLATIVGSAGVGKSRLVHEFLGYVA
                     GRARILRAQCLPYGEGITFWALTVAVMQASGAGPHEAREAARARLDGILGDDHQDVAE
                     RLAAVTGLSSQTFPLQETFWAVRRFLEILASVEPVVLVLDDIHWAEPTFLDLIDHVRE
                     TAEAPMLMVCPARHELLDHRADWALSKPNAVRLHLESLSIAEAEQVVTQLLSSAEVPP
                     GVRESLARAVGGNPLYLEQTLSMLLERGVLRSQEDRWHLAGDIDSLDIPPTISALITA
                     RLDQLEDEERIVMGRASVIGETFYTGAVAHLSPDSLSPLVPSSLHTLTTKELVAPVVD
                     ATFVGEEAFCFRHLMIRDCAYGSLLKRTRAELHERFVEWLESVTGARAGDFEEIIGYH
                     LEQACSHRKELGPLDETGHGLAARASHSLGSAGRQALSRRDLPAATNLLERAVAIEQE
                     RAPARLALIPDLAEALIDSGEFASADGYLEQAIKASRESGDERLEADAKIMRMFVRYS
                     TDPEGWSEEVVDVAEEAMSVLERHGDHAALAKAWRLIGSIHGLNCRYAQAEDAMRRAV
                     LEAREAGDRRQELLCLPTYALSAAYGPTPVPSAIRRCEEVLEQSTDSKSAVALVLCAL
                     AHLQGLAGQFESAREHYQRSRSIYQQLGMKIAAALVSLDSAPVEMLAGDPAAAARE"
     gene            complement(2892..3887)
                     /locus_tag="VH419_15250"
     CDS             complement(2892..3887)
                     /locus_tag="VH419_15250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020657320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HEY7045025.1"
                     /translation="MKKFVNDPREFVAEMLTGIELASGGSLKYEPKYNLIYRADIPKD
                     DKVSIIQGSGSGHEPAHTMIVGPGMLDGACPGDVFAGPPVEFVYETTKLLYSPKGILH
                     IVNNYTGDRTAWEMAKEMCEAEGMKVGALLVNDDVAVKDSTWTVGRRGVAGNFFVIKA
                     TGAASENGAELDELVRIGEKVIANVRTMGIALSSCTPPVRGTPLFDIADDEMEIGIGI
                     HGEPGRERTKLGSANEIVDILLDAVIPDLPFESGDEIALMVNGLGGTPISELYLLYGL
                     AHKRLADRGISVFRSYVNEYCTSLEMAGAHLTALKVDDELKQLLLAPAEISNRVF"
     gene            complement(3884..4552)
                     /gene="dhaL"
                     /locus_tag="VH419_15255"
     CDS             complement(3884..4552)
                     /gene="dhaL"
                     /locus_tag="VH419_15255"
                     /inference="COORDINATES: protein motif:HMM:TIGR02365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HEY7045026.1"
                     /translation="MARTALDEVDLVINTMATTIVDNADYFAQLDAVVGDGDFGYSLR
                     NGFEVVLADYETFDHSSVGAILKRIGFVIAGKVGGVSGPIWGTAFLRAAAAADDKTEL
                     ASADIIDILRAAIEGIKQRGEASLGDKTLLDALVPAVDSLEQSLAAGVSDDGVAAIQD
                     AADVAVKAAEQTLGMLAMRGRAAYTGERSRDSVDAGATAVGVILQAISAAWREAHTEE
                     ETEA"
     gene            complement(4530..5369)
                     /locus_tag="VH419_15260"
     CDS             complement(4530..5369)
                     /locus_tag="VH419_15260"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEY7045027.1"
                     /translation="MVNALIFDCDGVLADTERDGHRVAFNRTFAEFDLPIEWSEATYA
                     EKLKTAGGKERMATELTPEFSRANNLPEASVEQAALLAQLHKRKTAIYTDMVAAGMLP
                     TRPGITRIIAEAQDNGWEVAVASTSAEPSVRAILERAAGPERAARFDVVLAGDIVAHK
                     KPAPDIYLLALERLEVSPAEALVIEDSRNGLLAATAAGMRCVMTVNGYTEEEDNSEAI
                     LVVSSLGDPDGERTRVIVNRGSASPGNYITLADLERCLAPDSDTRPRNGIGGGSYGTH
                     CAR"
     gene            complement(5363..6214)
                     /locus_tag="VH419_15265"
     CDS             complement(5363..6214)
                     /locus_tag="VH419_15265"
                     /inference="COORDINATES: protein motif:HMM:NF013296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HEY7045028.1"
                     /translation="MPVVPTQDILARAFDQRYGVAAINVVNDLSMEAVLAAAEELRSP
                     LIVQTSVKTVKSLGAEVLYAMWRAMTDTIDVPVALHLDHCPERETITLCLEKGWNSVL
                     FDASHLSVEDNKRQCAEVVAEARPYGALVEGEIEGFKRVEDIGDEQVESEQSLETAVD
                     FIKSTGVDVFAPAIGNAHGMYQRAPKLDSQRVTDIVEATGIPVALHGGTGMTAEQFSD
                     LISRGCAKVNISTALKIQLMQSGYDFMTENPGTYDPPSQFRVQRAAVMDMTREHIRLF
                     GSEGQAW"
     gene            complement(6214..7179)
                     /locus_tag="VH419_15270"
     CDS             complement(6214..7179)
                     /locus_tag="VH419_15270"
                     /inference="COORDINATES: protein motif:HMM:NF016114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HEY7045029.1"
                     /translation="MVDLDREEHELLARVARRWYVDRRTQGEIAAEFGLSRPKVQRLL
                     DRARATGVVEVHIEVPMGMDLDLEHRFVDLFGLKAAIVSPSVPDPDEQRVLVARRAAR
                     YLEQLVGDGSVVAVSHGRDIGAVPRQFRPIGPIDCVFASAMGGSPRVDAPTNPNETCR
                     SMAERSGGRAESLYAPAYVESAEVRDRLLEQDAVAHALKVAAGADVALVGMGGTDDAC
                     TMVRSGCLTRAEIRSLRRQGAVGDVLGNYVDIDGEHIVAPHSHRLIGLSIDDLRGIPT
                     VVAVVSGDEKPRAILGVLRAGIVDVLILDESNAQTVLDLAQTGDN"
     gene            complement(7413..8657)
                     /gene="rocD"
                     /locus_tag="VH419_15275"
     CDS             complement(7413..8657)
                     /gene="rocD"
                     /locus_tag="VH419_15275"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009075701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine aminotransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HEY7045030.1"
                     /translation="MTVTTRTPEAISSPLSDTEAHIELNEAYAAHNYHPLRVVLSAGE
                     GAWVTDVEGRRFLDCLAGYSALNFGHAHPRLVARAREQVGRLTLTSRAFYNDELGPFA
                     RDLAQLTGKEMILPMNSGAEAVETAIKVARKWGYLVKGVPADQATIVAMGGNFHGRTT
                     TIVSFSTDSVATAHYAPYTPGFRLVQFGDFADLEAAIDESTVAVLLEPIQGEAGVVIP
                     PEGYLQQVRTLCNSAGVLLIADEIQSGLARTGRTFACDHEEVVPDVYVLGKALGGGLY
                     PVSAIAADGDVLQVITPGSHGSTFGGNPLAAAIGREVVAMLDTGEFQQRAETLGERLA
                     AGLGELVGQGVDTVRARGLWAGVDISPELSTGRHVCEQLLDQGILAKEAHGQTVRLAP
                     PLVASEDDIDLLVSAFRTVCTG"
     gene            8895..9806
                     /locus_tag="VH419_15280"
     CDS             8895..9806
                     /locus_tag="VH419_15280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7045031.1"
                     /translation="MPTLIVPRGRVDLSRVGMRAAAVRLVVDSGPRGVIDVDVEGVVV
                     PMLVHANAGFTVMLTHDALRRINGTRVAKETEFGLGADLRVSDLGRGTTTLGSLAVAG
                     SRLSDVRCEVFQLPTTNWEGMLGVGWLAAVEPVVDLANRWLFVPTARQRAEIDERLGA
                     DATRVELTRDEMSDRYTSLVSVDSGGATSVTFVASTVADTIVDVEYARRCGIELGPVV
                     GEEHGPTGAVVANHRAKEPVRFYSSGDLIATLTPEVHDVYAYSGAERPTSSDAIGGYL
                     GADLLLATGAILDFGQPRAAGGRVSGR"
     gene            complement(9795..11477)
                     /locus_tag="VH419_15285"
     CDS             complement(9795..11477)
                     /locus_tag="VH419_15285"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY7045032.1"
                     /translation="MATPPDLPADTGAGDQRASQAPWLPLIVIVLAQLQMSINISVLP
                     VSLGPISEDLDAPATATATALLVYSLFVAAFVMLGAKIGKLAGERRVLQASVVVHGVA
                     MGLMAASTDASTMNTAQAIAGVAAAAAVPTWVVLIAANYHGRQQETALGVLAGIPAVA
                     SAVTFVVAGFLATALSWRYSYGLIVGLAVVVLILSYRLTPIPRQRSIRIDVVGVVLSA
                     AAIGLMLFGFSNLQTWGLLAATDEAPFSLLHVSPAPMFILGGIVLGQAFFAWSHRRVA
                     AEKQPLLAMEVLDSREEKSAVIAFLVAGSLGLAVGFLIPLYVQIVQDRTPLATAVAIL
                     PYAVAIAAAGVASVRLYDRRSPRTLGIVSFVLIAVGLVVVAFTIGQDWSTVGVILGLL
                     LVGLGEGTLLTLLFNVLVSASPKRLAGDVGALRGVANNVSNAFGAALASVVAVGLLSV
                     FLTSALSRSELPFEIRAEGAFGNVDFVTDDELRSELGAISATPEQVEEAVAINEDARL
                     NALRAAFLIVAGISLLSIFPAMRLPKYVPGELSAADIVSEAGPGETQPASPT"
     gene            complement(11856..12248)
                     /locus_tag="VH419_15290"
     CDS             complement(11856..12248)
                     /locus_tag="VH419_15290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020578460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY7045033.1"
                     /translation="MARLGDIVFDCEKPASLARWWASTLDGYRVAPYDAEELERLRNM
                     GVFDPEDDPSVVVEPSLGDGPRYLFMRVPEPKRHKNRLHLDLRSDNLEAEVTRLVDRG
                     AVELYRIDTNLTMADPEGNEFCINAGPV"
     gene            12550..13137
                     /locus_tag="VH419_15295"
     CDS             12550..13137
                     /locus_tag="VH419_15295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012921153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY7045034.1"
                     /translation="MSDFIIKALTPETFDDFAALVERNKGMFASCWCTKFHPDCPEKG
                     QSAEGNRALKQRLVDDGIAHASLVYDGDRAVAWAEYGSPEELPSIQHRKEYLATAERL
                     PDYRVTCIQVERGLRGQGLAAIALRGAVELIAKAGGGLVEGYPHDTGGVRKKNSSFLY
                     NGTRTMYEREGFTYDRPKGQGNCVMVRQVAARTRD"
     gene            complement(13192..>15955)
                     /locus_tag="VH419_15300"
     CDS             complement(13192..>15955)
                     /locus_tag="VH419_15300"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HEY7045035.1"
                     /translation="GDLAINEVMTFDGLGRATMVQRANADGGWDVVETTSNVRGSHRT
                     VTVPYRGMRDDDTGKISETYDPLGRLTRRVTSPDSSVSAWFYDEPAAIGSRMFHEQFH
                     TTMRSVDPWGRERWAAFDEKGRVRSVAEPLPDGDGAVTAGKPTLHTDIVYAALRTSII
                     SNSHQGGAQWDQIRHFHVDGLGRLVAQSLPERAQPHSYPETPSIRWSDLYTYDLRGNM
                     TSHTDARGVITHYDYHDDPLDRLHRVTRDISHVTQTYPVKPVPPVEYGYGDLVDPRRV
                     TIESSGALTRTNTYDSGGLHETSLAWADLPNGPLTLTYERETLGRITRTILPAQYGQP
                     GDHRPTLGYTYGVGGSIARLTVDGSDLASEIHHDAGGNLSALTLGPAGAGQTIENYSW
                     DLEHGLLEHQSLTRNGTTLLDLWYDYQRHMPDVVAGKTGQVTVVTNAIAPVTTAEYVY
                     DNLGRVTRVGHGAQGGSFDWSQEYTYDAYGNRLDVTATNSDSAHPVPADGDTGLTADR
                     STNHVTATGWAYDAAGNLVERPVAWGTLRFGYDAAGRLVTIADGTGNVLQEHLYGACH
                     RRRATYTPSTGTTLLYAWEGDNVIAQYFAEPPTTTGGVAGKDGRPTWFVGAHFLGGRQ
                     LVTVGPRYQPTWSTQLHPDRIGTASVTGPQGDPATDATIAWLPYGSQQLQDSAAIPYI
                     GAGFAGYWPQDLELNYAQHRFYDPRAGRFTSPDPLGNQAFDLLNPQTLNPYSYVVNDP
                     VNRTDPLGLMAILGCIQAGDGPMECRWYYDPGPLGTSWSDPEERPGTNGGAGGRGGGV
                     GPSLLMGFHKGPTHIEWRAAPDPEFLVDYAKYAEQMVKTAKPDANHVIKLVQGINSAT
                     RAGRAYAEAAWQIAKPALEHAAQQGVNVGEVIFNYAGRIVLAPMAIFAIDPALYERDP
                     ANRWVG"
BASE COUNT         2569 a         5220 c         5349 g         2817 t
ORIGIN      
        1 ttctcgcgcg gcagccgcgg gatctccggc cagcatctcg acgggcgcgg agtccaacga
       61 cacgagcgcc gcggcgatct tcatgcccag ctgctgatag atcgagcggc ttcgttgata
      121 gtgctcgcga gcagactcga actggccggc cagcccttga aggtgagcga gcgcgcacag
      181 gaccagcgcg acggcactct tcgagtcggt ggactgttcc agcacctcct cgcaccgtcg
      241 gatggccgac ggaaccggtg tggggccgta cgccgcggac agtgcatacg tcggaaggca
      301 caagagttcc tgtcgccggt ctccggcctc cctcgcctcc agcacggctc ggcgcatggc
      361 gtcctcggcc tgggcgtacc ggcagttcag gccgtgaatc gacccgatca atcgccaagc
      421 cttggccagc gccgcgtgat caccgtgccg ttcaaggacc gacattgcct cttcggcgac
      481 gtcgaccact tcctccgacc agccttcggg gtcagtggag tagcggacga acatgcgcat
      541 gatcttcgcg tccgcctcga gccgctcgtc gccggactcc cgactggcct tgatcgcctg
      601 ctcgaggtag ccgtcggcgc tcgcgaactc accgctgtcg atcaacgctt cggccaggtc
      661 cggaatcagc gcgaggcgcg cgggagctcg ttcctgctcg atggcgaccg cacgctccag
      721 caggttcgtt gcggcgggga ggtcgcgccg agacagcgcc tgacggccgg cagagcccag
      781 actgtgtgac gcacgagcgg cgagtccgtg accggtttcg tcgagtggtc ccagctcttt
      841 ccggtgggaa caggcctgct cgaggtggta gccgatgatc tcttcgaagt cgccggcccg
      901 agcgccggtc acgctctcca gccattcgac gaagcgctcg tgcagctcgg cacgcgtacg
      961 tttcaagagc gacccgtacg cacagtcgcg gatcattagg tgacggaagc agaacgcctc
     1021 ctcaccgacg aatgtggcgt cgacgactgg agcaaccagt tccttggtcg tgagcgtgtg
     1081 cagactcgag ggcacgagtg gcgacaggct gtcgggcgac agatgtgcga ccgcgccggt
     1141 gtagaacgtc tcgccgatga cggacgctct acccatcacg atccgctctt cgtcctcgag
     1201 ttggtccagc cgcgcggtga tgagggccga gatcgtcggc ggaatgtcga gcgagtcgat
     1261 gtccccagcc aggtgccatc gatcttcctg tgagcgcagc actccccgct ccagcagcat
     1321 ggagagggtc tgctccaggt agaggggatt tccgccgacc gcgcgcgcga gactctcccg
     1381 tacgccgggt gggacctcgg ctgaactcaa caactgggtg acgacctgtt cggcctcggc
     1441 gatcgaaagc gactccaggt gcagccgcac cgcgttgggc ttggaaagag cccagtccgc
     1501 tcgatggtcg aggagctcgt gacgggcggg gcacaccatg agcatgggtg cctcggcggt
     1561 ctcgcggacg tggtcgatga gatccagaaa cgtcggctcg gcccagtgga tgtcgtcgag
     1621 gaccagtacg acgggctcga cgctcgccag gatctccaag aaccggcgta ccgcccagaa
     1681 tgtctcctgg agcggaaacg tctgtgatga gaggccagtg accgccgcca gccgctccgc
     1741 gacatcctga tggtcatccc cgagaatgcc gtccagcctt gctcgggcag cctccctcgc
     1801 ttcatgaggg ccggccccgc tcgcctgcat gaccgcgaca gtgagcgccc agaacgtgat
     1861 cccctccccg tagggcaggc actgagctcg gagaatccgg gcacgcccgg cgacgtaccc
     1921 gaggaactcg tgcaccagcc gggacttccc gacgccagca ctgccgacga tcgtcgcgag
     1981 ttggcaccgt cgcccggtga cggcttcgtc gaagcgttcc cggaggagct ggagctcctg
     2041 ctgccggccg accatgggcg tgtcgagccg gcgaggcacc gccgacgcgc cggggtcgac
     2101 cccgaggaga agataggccg gcactggttc cgccttgccc ttgagcggga gcgggtcgag
     2161 gcgctcgact gtgactgcct ccttgaccag ccggtacgtg tctgacccga tcaagatccc
     2221 gttctcggtc gctgcctgct cgagccgggc ggcgacgttc accgcgtcgc cggtgaccaa
     2281 ccgctgtccc gccgccgggt caccggcgac cacctcgcca gtgttcacgc cggtgcggtt
     2341 cgccatccga actccccacc tgcgcatcag ttcttcgttg agccggagca acgacttccg
     2401 catgtccacc gcggcacgga ctgcccgaag tgcgtcgtcc tcgtgcgccc gcggcagacc
     2461 gaaaaccgcc atgacggcgt ctccgatgaa cttctcgacg gtgcccccgt gctgctcgag
     2521 caccactcgc atctcgtcga agtacctcgt catcacctca cggagcgctt cggagtcgag
     2581 gtgttcgccg aggctggtcg agcctttgag gtcgcagaag acgatggaga cggtcttgcg
     2641 cacctcccgg acaggggcgc ccaacggtgt gccgcagaag ccgcagagcc gaaacctcgg
     2701 cgggttggtc tcaccgcagc cggggcagac gggcatggct cagcgtaggt ctcgccgctc
     2761 caccacgtcc gtcgagtcgt cagaagggca cgggactgtc gccctctgta atcgtgacgg
     2821 tgtagtcgtg acggctgata tggcagaagt gtgtggagcc ctggccccac acacctctga
     2881 caactcggcg gtcagaagac tcggttgctg atctcggccg gagccaacaa cagctgcttc
     2941 agctcgtcat ccaccttcag cgccgtcaga tgagcgcccg ccatctccaa cgacgtgcag
     3001 tactcgttga cgtagcttcg gaagaccgag atgcccctgt cggcgagtcg cttgtgagcc
     3061 agcccgtaca gcaggtacag ctcgctgatc ggggttccac cgaggccgtt gaccatcaac
     3121 gcgatctcgt caccggactc gaacggcagg tcggggatca ccgcgtcgag caggatgtcg
     3181 acaatctcgt tggcggatcc gagcttggtt cgctcgcggc cgggctcgcc gtggatcccg
     3241 ataccgatct ccatctcgtc gtcagcgatg tcgaacagcg gtgtcccccg caccggagga
     3301 gtgcacgagc tcagtgcgat gcccatggtc cgcacgttgg cgatgacctt ctcgccgata
     3361 cgcaccagct cgtcgagctc ggcgccgttc tcggacgcgg cccctgtcgc cttgatgacg
     3421 aagaagttgc ccgccacacc gcggcgtccg accgtccagg tggagtcctt caccgcgacg
     3481 tcgtcgttga ccaggagggc gccgaccttc atcccctcgg cctcgcacat ctccttcgcc
     3541 atttcccagg cggtgcggtc gcctgtgtag ttgttgacga tgtgcaggat ccccttcggg
     3601 gagtagagca acttggtggt ctcgtagacg aactcgacgg ggggaccggc gaagacgtca
     3661 cccgggcagg ctccgtcgag catgcccgga ccgacgatca tcgtgtgcgc cggctcgtgt
     3721 cctgaccccg agccctggat gatgctgacc ttgtcgtcct tggggatgtc ggcacgatag
     3781 atcaggttgt acttcggctc gtacttgagt gacccgccac tcgcgagctc gatgccggtg
     3841 agcatctcgg ccacgaactc gcgcgggtcg ttgacgaact tcttcacgcc tcggtctcct
     3901 cttccgtgtg tgcttcgcgc caggcggcgc tgattgcttg caggatcacg cccacggcgg
     3961 tggcccccgc gtccaccgag tcgcggctgc gctcgcccgt gtaggcggca cgtccgcgca
     4021 tcgcgagcat gcccagggtc tgctcagcgg ctttgacggc gacgtcagct gcgtcctgga
     4081 tggcggcgac gccgtcgtcg ctgacacctg cagcgagact ctgttcgagg ctgtccactg
     4141 cggggacgag cgcatcgagc agtgtcttgt ccccgagtga cgcctcaccg cgttgcttga
     4201 ttccctcgat agcggcgcgc aggatgtcga tgatgtcggc ggacgccagc tctgtcttgt
     4261 cgtccgcggc agcagcggct cgaaggaatg ccgtgcccca gatcgggcca ctcacgccac
     4321 cgaccttgcc ggcgatcacg aacccgatcc gcttgaggat cgctccgacg ctgctgtggt
     4381 cgaacgtctc gtagtcggca agcacgacct cgaacccgtt cctgagcgag tagccgaagt
     4441 cgccgtcacc gacgacggcg tcgagctggg cgaagtagtc tgcgttgtcg acgatcgtcg
     4501 tggccatcgt gttgatgacg aggtcgacct catcgagcgc agtgcgtgcc atacgaacct
     4561 cctccgatgc cgttgcgtgg tcgggtgtcc gaatcgggcg cgagacaccg ctccagatct
     4621 gccagggtga tgtagttgcc gggggaggct gaaccacggt tgacgatgac gcgcgtccgc
     4681 tccccatccg ggtcgcccaa tgacgagacg accaggatcg cttcgctgtt gtcctcctct
     4741 tctgtgtacc cgttgaccgt catcacgcaa cgcattcctg cggcggtggc cgccaacagg
     4801 ccgttgcgcg agtcctcgat caccagcgcc tccgccggcg agacctccaa ccgttccagg
     4861 gccagcaggt agatgtccgg cgcaggcttc ttgtgggcga cgatgtctcc ggccagcacg
     4921 acgtcgaatc gcgcggcgcg ctcgggcccg gctgcccgct cgaggatggc ccgcaccgat
     4981 ggctctgccg acgtcgacgc aaccgcgacc tcccagccgt tgtcctgcgc ctcggcgatg
     5041 atccgcgtga tgcctggccg ggtcggcagc atcccggcgg cgaccatgtc ggtgtagatg
     5101 gctgtcttgc gcttgtgcag ctgggccagc agcgctgcct gctccacact ggcctccggg
     5161 agattgttcg ctcggctgaa ctccggtgtc agttcggtag ccatccgctc cttgccgccg
     5221 gcggtcttga gcttctcggc gtacgtcgct tccgaccact cgatgggcag gtcgaactcg
     5281 gcaaaggttc ggttgaaggc gacgcggtgc ccgtcacgtt ccgtgtcggc cagtacgccg
     5341 tcacagtcga agatgagggc gttcaccatg cctgaccctc gctgccgaac aggcggatgt
     5401 gctcgcgcgt catgtccatc actgccgctc gctgcacgcg gaactgtgac ggcgggtcgt
     5461 acgtgccagg gttctcggtc atgaagtcat aaccggactg catcagctgg atcttcagag
     5521 cggtcgagat gttcaccttg gcgcacccgc gggagatgag atcgctgaac tgctcggcgg
     5581 tcatgccagt accaccgtgc agggcgaccg ggatgccggt ggcctccacg atgtccgtga
     5641 cgcgttgact gtccagcttc ggtgcgcgtt ggtacatgcc atgcgcgttg ccgatcgccg
     5701 gtgcgaacac atcgaccccc gtcgacttga tgaagtcgac cgcggtctcc agcgactgct
     5761 ccgactcgac ctgttcatcg ccgatgtcct cgacccgctt gaagccctcg atctcaccct
     5821 cgacgagcgc gccgtaggga cgcgcctcag cgacgacctc ggcacactga cgcttgttgt
     5881 cctccacgct caggtgtgag gcgtcgaaca gcaccgagtt ccaccccttc tccagacaca
     5941 gcgtgatcgt ctcgcgctca gggcagtggt ccaggtgcag ggccaccggc acgtcgatcg
     6001 tgtcggtcat ggcgcgccac atcgcgtaga ggacttccgc gccgagagac ttcacggtct
     6061 tcaccgatgt ctgcacgatc agcggtgagc gcagctcctc ggccgccgcc agcaccgctt
     6121 ccatgctgag gtcgttgacg acgttgatcg ccgcaacgcc gtaccgctgg tcgaaggcgc
     6181 gggccaggat gtcctgggtg gggacgacgg gcatcagttg tctcctgtct gggcaaggtc
     6241 gaggaccgtt tgcgcattcg actcgtccag gatgagtacg tccacgatcc cggcacggag
     6301 cacacccagg atcgcgcggg gcttctcgtc gccgctcacc actgcgacca cggtcgggat
     6361 cccgcgcagg tcgtcgatgg acagtccgat gagtcgatgg ctgtgcggtg cgacgatgtg
     6421 ctcgccgtcg atgtcgacgt agttcccgag tacgtcgccg acggcacctt gtcgacgcag
     6481 gctgcgtatc tccgctctgg tgagacaacc actgcggacc atcgtgcacg catcgtcggt
     6541 gccgcccatc ccgaccaggg cgacatccgc acctgctgcc accttcagag cgtgtgccac
     6601 cgcatcctgt tcgagcagcc gatcgcgaac ctctgcgctc tccacgtacg ccggcgcgta
     6661 gaggctctca gcgcgaccgc cgctccgctc ggccatcgag cgacaggtct cgttcgggtt
     6721 ggtgggggcg tcgacgcggg gtgacccgcc catggcgctg gcgaagacgc agtcgatcgg
     6781 gccgatgggg cggaactgcc gcggtacggc gccgatgtcc cgcccgtggc tgacggccac
     6841 cacgctgccg tcacccacca gctgctcgag gtagcgagca gcacgacgag cgacaagcac
     6901 ccgttgttcg tccggatcgg gaacgctcgg gctgacgatt gctgccttga ggccgaacaa
     6961 atcgacgaac cgatgctcga gatcgagatc catccccatg gggacctcga tgtgcacctc
     7021 gaccacgccg gtggctctgg cacgatccag gagccgttga accttgggac gcgacaagcc
     7081 gaactcggcg gcgatctctc cctgggtccg tcgatcgacg taccaccgcc gtgcgacacg
     7141 ggccagcagc tcgtgctctt cccgatcaag gtcgaccatc agggatctct cgtccgactg
     7201 agcaaatgct cagttgcgcg cagatgctca acgtagcagg cgctgccgat cgttgggcag
     7261 aggctgcctg acccgtgggg gtctgggcat cttggtccgg ctcgtgccgg actgtgcccg
     7321 ccggctgacg tcctcggttg gaagaggagg ctgtggtggc gacgccggcg gcgcgcagca
     7381 aggccagggt tcgatgaccc ccatgggccg aatcagccgg tgcagacggt gcggaacgcg
     7441 ctgaccagga gatcgatgtc gtcctcggag gcgacgagcg gcggagccag tcggaccgtc
     7501 tgcccgtgcg cctccttggc gaggatgcct tggtcgagca gttgctcgca cacgtgccgg
     7561 ccggtgctca gctcggggct gatgtcgacg cccgcccaca gcccacgtgc tcgtaccgtg
     7621 tcgacgccct ggccgaccag ctcgccgagg ccggcagcaa gccgttcacc gagcgtctcc
     7681 gcgcgctgtt ggaactcacc cgtgtccagc atcgcgacca cctcgcggcc gatcgcggcg
     7741 gccagcgggt tgccgccgaa ggtcgagcca tgggaccccg gggtgatcac ctggagcacg
     7801 tcgccatcgg ctgcgatcgc cgacaccggg tagaggccgc ccccgagcgc cttgccgagg
     7861 acatagacgt cgggcacgac ctcctcatgg tcgcaggcga aggtccgccc ggtccgggcg
     7921 aggccggact ggatctcatc ggcgatcagc agcacaccgg cggagttgca cagagtgcgc
     7981 acctgctgga ggtagccctc gggcggaatg accacaccgg cctcgccctg gatgggctcg
     8041 aggagcacgg cgaccgtgga ctcgtcgatc gctgcctcga ggtcggcgaa atcgccgaac
     8101 tgcacgaggc ggaagcccgg tgtgtacggc gcgtagtggg cggtcgcgac ggaatcagtc
     8161 gagaacgaca cgatcgtcgt tgtccggccg tggaagttcc ctcccatcgc gacgatcgtc
     8221 gcctggtccg ccgggacgcc cttgaccaga tagccccact ttcgggcgac cttgatcgcg
     8281 gtctcgacgg cctcggcgcc ggagttcatc ggcaggatca tctccttgcc ggtcaactgc
     8341 gcgaggtccc gggcgaaggg gccaagttcg tcgttgtaga acgcccgcga ggtcagcgtg
     8401 agccggccga cctgctctcg cgcgcgagcc accaggcggg gatgcgcgtg gccgaagttc
     8461 agcgcgctgt agccggccag gcagtcgagg aagcgacgtc cctcgacgtc ggtgacccag
     8521 gcgccctcac cggccgacag cacgacgcgc agcgggtggt agttgtgcgc agcgtaggcc
     8581 tcgttgagct cgatgtgggc ttcggtgtcg ctgagtgggg agctgatcgc ctcaggcgtg
     8641 cgggtggtga ccgtcatgcc cggcatcgtc tcatcccgac ctgcggtacc tcgcgggatc
     8701 ctgccgattc ttggcgcaga gtcgcgggcg gcggacgagg agacgctgtc gagcggcaac
     8761 cagccggtgc actgctttca gtcggcgctg tcgcggggca ctaggttgag tcggtgactc
     8821 ctgaccttgg gccaggacgt ctcgtgagag gacttcggca cgcagatctg ggcgtgagag
     8881 cggaggacgg ggtcgtgccg acgctgatcg tgcctcgtgg gcgtgtcgat ctgtcgcgcg
     8941 ttggcatgcg cgcggcagcc gttcgcctcg tcgtcgactc gggtccgcga ggtgtcatcg
     9001 atgtcgacgt cgagggcgtc gtcgtaccga tgctcgtgca cgccaatgcg ggcttcaccg
     9061 tcatgctgac gcacgacgcc ctgcgcagga tcaacggcac ccgcgtggcg aaggagaccg
     9121 agttcggcct cggcgccgat ctgcgggtca gcgacctcgg ccgggggacg acgacgcttg
     9181 gttcgcttgc tgtggccggt tcccggctga gcgacgttcg gtgcgaggtg ttccagctgc
     9241 cgacgacgaa ctgggagggg atgctcggtg tcgggtggct ggcggcggtc gagccggtcg
     9301 tcgatctggc gaaccgctgg ctgttcgtgc cgaccgcgcg gcagcgcgct gaaatcgacg
     9361 aacgcctcgg cgcggacgcg acccgagtgg agctgacccg cgacgagatg tcggatcgct
     9421 acacctccct ggtttcggtg gactcgggcg gggcaacgtc agtcacgttc gtcgccagca
     9481 cggtcgccga caccattgtc gatgtcgagt acgcccggcg gtgcggcatc gagctcggac
     9541 cggtcgtcgg cgaggaacac ggtccgaccg gtgccgtggt cgcgaaccac cgagccaagg
     9601 aaccggtgag gttctactcg tccggtgacc tgatcgcgac cctcacaccc gaggtgcacg
     9661 acgtgtacgc ctacagcggc gccgagcgac caacgagttc tgacgcgatc ggcggctatc
     9721 tcggtgccga ccttctcctc gcgaccggtg ccatcctcga cttcggtcag cctcgggcgg
     9781 ccgggggacg ggtctcaggt cggtgaggct ggctgcgtct cgcctggtcc cgcctcactc
     9841 acgatgtccg ctgccgacag ctcgcctggc acgtacttcg gcaatctcat ggctgggaag
     9901 atcgacaaca acgagatgcc cgccacgatc aagaacgctg cgcgcagggc gttgagtcgg
     9961 gcgtcttcgt tgatcgcgac cgcctcttcc acttgctccg gcgtcgccga gatcgcaccg
    10021 agctcgctcc tcaactcgtc atcggtcacg aagtcgacgt tgccgaacgc gccctcggcc
    10081 cggatctcga agggcagctc ggagcggctg agggctgacg tgagaaacac gctcaacaac
    10141 ccgaccgcca cgacgctggc caacgccgcg ccgaacgcgt tcgagacgtt gttcgccacg
    10201 ccccgcaacg cgcccacgtc gccggcgagt cgcttgggag atgcggatac gagcacgttg
    10261 aacaacagcg tgagcagcgt gccttcaccg agaccgacga ggagcagacc caggatgacc
    10321 cccaccgtgc tccagtcctg gccgatggtg aaggcgacca cgaccagccc gaccgcaatg
    10381 aggacaaacg agacgatacc gagagtgcgc ggcgaccggc gatcgtagag tcggaccgag
    10441 gcaacaccag ctgctgcgat ggcaacggcg tacgggagga tggccaccgc cgtggccagg
    10501 ggcgtgcggt cttgaacgat ctgcacatac agcgggatga gaaaacccac cgcgagaccg
    10561 aggctccccg cgacgaggaa cgcgatgacg gcactcttct cctccctgct gtccaagacc
    10621 tccatcgcga gcagcggctg cttctcggcg gccacccgcc tgtgtgacca cgcgaagaag
    10681 gcttgaccga gcacgatccc gccaaggatg aacatcggcg ctggtgacac gtgcagcagc
    10741 gagaacggtg cctcgtccgt cgcggcaagc agtccccagg tctggaggtt gctgaagccg
    10801 aagagcatga gcccgatcgc tgcggctgag agcacgaccc ccacaacgtc gatcctgatg
    10861 ctgcgttggc gggggatcgg cgtcaggcga tagctcaaga tgaggacgac gactgccagg
    10921 ccgacaatca gtccatacga gtagcgccag ctcagggcag tggcgaggaa gccggcgacg
    10981 acgaaggtga ccgcgctggc aaccgcgggg attccggcaa gaacgcccaa tgccgtctcc
    11041 tgctgccggc cgtggtagtt cgccgcgatc aggaccaccc acgtcggtac ggccgcggcg
    11101 gcagcaactc ccgcgatcgc ctgggctgtg ttcatcgtgc tcgcgtcggt gctggccgcc
    11161 atcaaaccca tcgccacgcc gtgcaccacg acacttgctt ggagcacccg gcgctcgccc
    11221 gcgagcttgc cgatcttcgc gcccagcatg acgaacgcgg cgacgaacag ggaatagaca
    11281 aggagcgcag tcgccgtcgc cgtcgccggc gcatccaggt cttcggagat cggcccgagc
    11341 gacacaggca agacgctgat gttgatggac atctggagtt gggcgagcac gatgacgatc
    11401 aatggcaacc agggcgcctg ggacgcgcgc tggtccccag cgccggtgtc ggccggcaga
    11461 tctggtggtg tcgccatgtg atccatctca gaggctgtga cgccgttgct ggcttcctcg
    11521 ccaggtgctc acgaactcag gagtctcctc tctcctgcgg tgaaccggta ctccgctcgt
    11581 cctcctgccg tatccacgcc gctcgtggtt gccaggccct ggacgggaat ccaaggactg
    11641 tgaggcacgc atcgaggtgc ggccggccac cttggatgca gtgcaacgcc acaagcggtg
    11701 caagctctgt cattggtagc aggatgccgc atcatcgagg ttgcgaacgg cggtggccgt
    11761 ggtggagatg cgggagagtc cgctgtcgag ggcggcgggt gcgccgaaca agccgctcga
    11821 aaacgtccgc ccataccgat gatctgacgt acgagtcaca ctggaccggc gttgatgcag
    11881 aattcgttgc cttcggggtc ggccatggtc aggttggtgt cgatccggta cagctccact
    11941 gctccgcggt cgacgagccg ggtgacctct gcctcgagat tgtcgctgcg cagatccagg
    12001 tgaagtcggt tcttgtgtcg cttcggctca ggaaccctca tgaacagata acggggcccg
    12061 tcgccgagcg acggttccac cacgacgctt ggatcgtcct caggatcgaa gacgcccatg
    12121 ttgcgcaatc gctccaactc ttcggcgtcg tagggtgcga cgcggtaccc atcaagcgtc
    12181 gatgcccacc agcgcgccag ggaggccggt ttctcacagt cgaagacgat gtcacccagg
    12241 cgagccaccg acccaagata gcgctccacg cagaccggaa cctggttgtg tcgtggcatg
    12301 tctacctggc gagcggccct tgggcgctgg ggctttgggc aagtgatccg atcgaaccac
    12361 gcgagtcggc ttcagatggt ccggactggc tgttgcatcc tcgtcaccag accaagcgct
    12421 ggctcttgca actcaaatgc tggctgcggg ctggcccgtt cgccgcagcc gaagagcgtg
    12481 cgcgtgcgcc gcctcggcag cgtgaactac cggacgccga acgtccgcaa tcgcaggtac
    12541 gttgctggca tgtccgactt catcatcaag gcgctcacgc cggagacgtt cgacgacttc
    12601 gcggctctcg tcgagcggaa caagggcatg ttcgccagct gctggtgcac gaagttccat
    12661 cccgactgtc cggagaaggg ccagagcgcc gaggggaacc gggcgctcaa gcaacgtctg
    12721 gtggacgacg ggatcgcgca cgcgtcgctg gtctacgacg gcgaccgggc cgtcgcctgg
    12781 gccgagtacg gatcacccga ggagctgccc agcatccagc accgcaagga gtacctggcc
    12841 actgctgagc gactgcccga ctaccgggtc acctgcatcc aggtcgagcg aggccttcgc
    12901 ggtcagggct tggcggcgat cgccctgcgc ggagccgtcg agctgatcgc gaaggccggc
    12961 ggcggcctgg tcgagggcta cccgcacgac accggcgggg tccggaagaa gaactcgtcg
    13021 ttcctctaca acggcacccg cacgatgtac gagcgcgagg gcttcaccta cgaccgaccc
    13081 aagggccagg gcaactgcgt gatggtgcgc caagtggccg cgcgcacgcg tgactgagcg
    13141 atcagccatg tggggcatgc ctgatcgggc caccagggcc gcgcgggttc gttatccgac
    13201 ccacctgttc gctgggtccc gctcgtagag ggcggggtcg atggcgaaga tcgccatggg
    13261 agcaaggacg atccggccgg cgtagttgaa gatgacctct ccgacgttca cgccttgctg
    13321 tgcggcatgt tctagcgctg gcttggctat ttgccatgca gcctcagcgt aggcgcgccc
    13381 cgccctggtg gcgctgttga tgccctgtac gagcttgatg acatggtttg cgtctggctt
    13441 ggcggttttg accatctgct ctgcgtactt ggcgtagtcg acgaggaact cggggtcggg
    13501 agcggctctc cactcgatgt gagtggggcc cttgtggaac cccatgagga gtgacgggcc
    13561 gacgcctccg ccgcgaccgc ctgctccgcc attcgtgcca ggtcgctcct cgggatcgga
    13621 ccacgaggtt ccgagggggc ctgggtcgta gtaccacctg cactccatcg gcccgtcccc
    13681 ggcctggatg caaccgagaa tcgccatcag tccgagcggg tctgtgcgat tcaccgggtc
    13741 gttgacgacg tagctgtacg ggttgagagt ctgcgggttc aggaggtcga aggcttgatt
    13801 gccgagcggg tcaggggagg tgaaccggcc agctcgtggg tcgtagaacc gatgttgtgc
    13861 atagttcagc tcgaggtcct gcggccagta cccggcgaag ccggctccga tgtatgggat
    13921 tgcggcgctg tcctggagct gttgtgagcc gtagggcagc cacgcgatgg ttgcgtctgt
    13981 tgcggggtct ccctgcgggc cggtcaccga cgctgtgccg atccggtccg ggtgcagctg
    14041 ggtgctccag gtcggctggt agcgaggccc taccgtgacc agctgacgac cgcccagaaa
    14101 gtgcgcgccg acgaaccagg tggggcggcc atccttgccc gcgactccgc ctgtggtggt
    14161 cggaggctcg gcgaagtact gcgcaatgac attgtcgcct tcccaggcgt acaacaacgt
    14221 cgtgcctgtt gatggagtgt aggtggcgcg tcggcggtgg cacgcgccgt agaggtgctc
    14281 ttggagcacg ttgcccgtgc cgtcggcgat cgtcaccagc cggcccgcgg cgtcgtaacc
    14341 gaaccggagg gttccccaag cgactggcct ttcgacgagg ttgcccgcgg cgtcgtacgc
    14401 ccatccggtt gccgtgacat ggttcgtcga gcggtccgca gtgagcccgg tgtctccgtc
    14461 ggctgggacc ggatgggccg aatcgctgtt ggtcgcggtg acatcgagcc ggttcccgta
    14521 ggcgtcgtag gtgtattcct gcgaccagtc gaagctgccg ccctgcgcgc cgtgtccgac
    14581 gcgggtcacc ctgccgagat tgtcgtacac atactccgct gtggtgaccg gcgcgatggc
    14641 gttggtgacc accgtcacct gtccggtctt gccggcaacg acgtcgggca tgtgccgctg
    14701 atagtcgtac cagaggtcta gcagggtcgt tccgttcctc gtcaggctct ggtgctcgag
    14761 gaggccgtgt tccagatccc aggagtagtt ctcaatagtt tgaccggcgc cagctggacc
    14821 gagcgtgagt gcgctgaggt tgccgccggc gtcatggtgg atctcactgg cgagatcgga
    14881 gccgtcgacg gtcagccgag cgattgaacc gccaaccccg tacgtgtatc ccagggtcgg
    14941 ccggtgatcc cctggctgcc cgtactgcgc tggaaggatc gttcgcgtga tgcgtcccag
    15001 cgtttcgcgt tcatacgtca aggtgagtgg gccgttggga aggtcggccc aggccaggga
    15061 cgtctcgtgg agtccaccgc tgtcgtaggt gttggtacgg gtgagcgcac cggatgactc
    15121 gatcgtgacc cggcgtgggt ccacgaggtc gccatagccg tactcgaccg gcggtaccgg
    15181 cttgaccggg taggtctgag tgacgtgcga gatgtcccgc gtgacgcggt ggagacggtc
    15241 gagtgggtcg tcgtggtagt cgtagtgcgt gatcacaccg cgggcgtcgg tgtgtgaggt
    15301 catgttgccg cgcaggtcat aggtgtacag gtcgctccac ctgatgcttg gggtctctgg
    15361 gtagctgtgc ggctgagcgc gctcgggaag gctttgggcg accagccgac cgagcccgtc
    15421 aacgtggaag tgccggatct ggtcccactg cgccccaccc tggtgagaat tcgagatgat
    15481 ggaggtccgc agtgccgcgt agacaatgtc ggtgtgcagc gtgggcttgc ccgcggtgac
    15541 ggccccgtct ccatcaggta gtggttccgc gaccgatcgg actcgacctt tctcgtcgaa
    15601 ggcagcccag cgctcccgtc cccaagggtc gactgagcgc atcgtcgtgt ggaactgctc
    15661 atggaacatc cgggagccga tcgctgctgg ctcgtcgtag aaccaggcgc tgacgctgct
    15721 gtcggggctg gtgacgcggc gggtcagtcg tccgagcggg tcgtaggtct cgctgatctt
    15781 gccggtgtcg tcgtcacgca tcccccggta cgggacggtg acggttctgt ggcttcctcg
    15841 cacgttcgag gtggtctcca cgacgtccca gccgccgtcc gcattcgctc gctgcaccat
    15901 ggtggcgcgt ccaaggccgt cgaacgtcat cacctcgttg atcgccaggt ccccg
//
LOCUS       DATBFZ010000501        13465 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_5467300, whole genome shotgun sequence.
ACCESSION   DATBFZ010000501 DATBFZ010000000
VERSION     DATBFZ010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 13465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13465
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5467300"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..898
                     /locus_tag="VH419_15305"
     CDS             <1..898
                     /locus_tag="VH419_15305"
                     /inference="COORDINATES: protein motif:HMM:TIGR03057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7045036.1"
                     /translation="PNFNALNCDQNPADDPNPVDDCGIGQVQQLVGAGIDQLVLSISD
                     RLSGVLGQAYTGSQDLYAGTQQLAHGTRKLADGSTKLVDGTVQLADGTVVLADGTRQL
                     ADGTVVLADGTRQLADGTVVLADGTRLLKNGAVRLHNGAGKLDDGGVKLSDGADQLSN
                     GATQLDDGSHQLLSGLTTAADGSNRLRAGLQKAADAAPQLSDGATKLSKQGTEKLIEA
                     GSDTTVEFGAKYAQIQAGAERAHDESMAFGAPQGADGLTAYTYEIRGEDGEGSRNWTR
                     GIAAIALFGLGAGAYALRRRFV"
     gene            complement(931..1725)
                     /locus_tag="VH419_15310"
     CDS             complement(931..1725)
                     /locus_tag="VH419_15310"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017933809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY7045037.1"
                     /translation="MRSEFRHVSDAEAQTANRADWDAEADSYQREHGTFLGDARFVWC
                     PEGLDEQDARLLGDVSGRRVLDLGCGAAQCARWLRTKHAAAVGVDLSQRMLQHGHRLD
                     DATGVNVPVVCGSATALPFGDDSFDAVCSAFGALPFVVDIERAFREVVRVLRADGLFA
                     FSVVHPVRRMFPDEPTSAGMTIVRSYFDRSAYVEVAHDGGATYVEPHHTLGDWIEAIT
                     ASGLDVESLVEPEWPPGHTRVWGGWGPVRGSLLPGTALFVTRAPAG"
     gene            1894..2829
                     /locus_tag="VH419_15315"
     CDS             1894..2829
                     /locus_tag="VH419_15315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009080934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY7045038.1"
                     /translation="MNLSHSRQAGPPAVALDDVSRVFGAVRAVDGLSLTIQPGEIVAL
                     LGPNGAGKTTTIDMILGLSTPTRGDVSVYGLRPVDAMVRGLVSAVMQSGGLLKDYTVE
                     ETVQLTASLFPSSRPVSEVMQRAGLEHIAGRRVGACSGGEQQRLRFALALLPDPELLI
                     LDEPTTGMDVTTRREFWSAIRRDAHRGRTVIFATHYLEEADAYADRVVLVRSGRLVAD
                     GTASQIKGLASGRTVRATIPESGDHADAVLRSLPGVDLVERRGDTVFVHGSDSDAVAR
                     LLLTSTDARDLEITSRNLEDAFVALTSDDLEGVPS"
     gene            2826..3665
                     /locus_tag="VH419_15320"
     CDS             2826..3665
                     /locus_tag="VH419_15320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017936351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY7045039.1"
                     /translation="MTTDMDTHMGPETSTLATAVGSIATSQRPTPALGGFSLTFVRLE
                     LRRMLRNRRTVIFTLIMPSMFFLLFGTDSSQQHDSVGRGNVTAYVLISMAVYGAMLAT
                     TSGGASVSLERAVGWSRQLRLTPLQPVAYIAVKVVVAMSLGLASVVVVSVTGSFFGPQ
                     LPLHVWVMSAALAWICSVVFAAFGLCMGYLLPSENVMQVLGPALALLSFAGGLFVPLD
                     QMGHTFSTLATITPVYGVGVVARYPLTSDGSLPIAVINVLAWTCVFVVGAAWRFRRDT
                     ARV"
     gene            3763..4911
                     /locus_tag="VH419_15325"
     CDS             3763..4911
                     /locus_tag="VH419_15325"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEY7045040.1"
                     /translation="MAGNPTTVPGISVPGAPVDTSFRWRFGWIFAAAWLVYLGQPLSE
                     MVHDSPGWRQFLGFASLAVFVALFLAAVVQGRRARRGLSVELPVWKRLAVVAGLLVCV
                     VGMVPAAGAAALTGVTFAAAVAAMTLPVRQGLVVVLALFASTELLVRVVPGWADKGYG
                     LSVILAGVAAGAFRAALERNAALLRAQQELADLAVEEERSRIARDLHDILGHSLTVIT
                     VKSELAQRLLDVDTERARAELADLEELCRDALADVRATALGVRGVSLAGEIATARTAL
                     ESAGIEATLPVAADAVSSRWREVFAWTIREGVTNVIRHSRACHCRVEMGSDHVSVSDD
                     GVGMDLGRVATGQGLSGLRQRVELAGAKLSTGPGEGGRGVRLLVEVPA"
     gene            4911..5552
                     /locus_tag="VH419_15330"
     CDS             4911..5552
                     /locus_tag="VH419_15330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009080931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY7045041.1"
                     /translation="MTQIHDTAETGVIRVLLADDQGLVRGAVAALLDLEPDLEVVADV
                     GAGDEVVDAALAARPDVALLDVEMPGLDGVAAARRLRTALPSCRILMVTTFGRPGYLR
                     QAMAAGASGFVVKDTPARQLADAVRRVHEGLRVVDPALAADSLTYGESPLTERERDVL
                     RAASDGGTVADIAATLHLSDGTVRNHLSSAIGKTGARTRAEAARVAADNGWLL"
     gene            complement(5661..6533)
                     /locus_tag="VH419_15335"
     CDS             complement(5661..6533)
                     /locus_tag="VH419_15335"
                     /inference="COORDINATES: protein motif:HMM:NF003738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacetylase"
                     /protein_id="HEY7045042.1"
                     /translation="MGLVGDPVRRRLASPVAEPPAGDASALRKFLARQRKVFVLTGAG
                     CSTESGIPDYRGLDGEWKRQRPVEYLPFVRDPLTRSRYWARSLIGWRAIGRAAPNDAH
                     RALAAMERTGAVGLLVTQNVDGLHQAAGSRRVVDLHGRLDRVTCLSCRQMAARATWQA
                     RLEDLNGDWSGLSAPVAPDGDADLDGLDFSRFVVPACPRCGGVIKPDVVFFGENVPPW
                     RHTQATAALVTSDAMLVVGSSLMVHSGYRYALAAHRRGIPVAAVNLGRTRADHLLTLK
                     VSAPAGSTLTAALS"
     gene            6603..7592
                     /locus_tag="VH419_15340"
     CDS             6603..7592
                     /locus_tag="VH419_15340"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03557 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEY7045043.1"
                     /translation="MGEAKIGYAAALEQFGPGEILDLCASAEKHGFSGVMAADHFQPW
                     IPQQGQASYVWNVLGALGQCTTGDLGPGVTCPTFRHHPAIVAQAASTLEAMYPGRTWL
                     GLGSGEALNEHVFGGYWPEAPERIAMMFEAIEVIQKLFTGEDVKHKGEYFRMHTTRLW
                     TRAAEPPPIYVATAGPITARRAGARCDGLITPGATTEKVAGVLARFDEGARRADKDPT
                     ALPKLLQLHLSWAPTDEQAMDNAVTEWPNGGMRFGKQDVRSPYDFAAMAALVRREDFE
                     GRMVISSDPDAHRQEIQRFLDLGITQVYLHNVGRNQTEWMDVFGREVLPKLTA"
     gene            complement(8039..8398)
                     /locus_tag="VH419_15345"
     CDS             complement(8039..8398)
                     /locus_tag="VH419_15345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7045044.1"
                     /translation="MTAPTGQPAHLAPADSAAGGFAIETSVLARLLGMKLLTLEGVVL
                     VSPAQVRQLQGANGTPDRSATGATPAKTVVPAATTPVTGRRSATTDHPGNRLAQAAHV
                     LEESSKELRQLGGSETR"
     gene            complement(8355..8549)
                     /locus_tag="VH419_15350"
     CDS             complement(8355..8549)
                     /locus_tag="VH419_15350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7045045.1"
                     /translation="MKAVTIAAFGLGYVFGTKAGRERYAQILEIAEKASRRLETYGNG
                     GERSSAGDRTDRSASASRAS"
     gene            complement(8546..8713)
                     /locus_tag="VH419_15355"
     CDS             complement(8546..8713)
                     /locus_tag="VH419_15355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7045046.1"
                     /translation="MNLFRKKSPWERLVGPVSDVAPRAAKSGLTALGAVAGLSLASAA
                     VSKARARWGSE"
     gene            complement(8759..9766)
                     /locus_tag="VH419_15360"
     CDS             complement(8759..9766)
                     /locus_tag="VH419_15360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7045047.1"
                     /translation="MTANRPKKATKRATKQAKQASNQTTQQAPQKVASLMALGVKDLP
                     RNASWLLAKALSPAHDARDKAVTAGASTADATGRAAQSLGGLVREAGSSIKDVMPTSN
                     TSIEHRLAQAREAMERAREAEDRALRSAEEASQRVAVARATAEEEKAHLAEVRRTQAE
                     NVSLRVDEARRAAESEIDAVRTAAQQEADAAIARQQAQADERMAQARSEAERAQELAD
                     REVATATEQLAEARRLAEEATSAAEAVARAAKEERKRLSPTVKTDRRSSERAVANRNG
                     RSRATKTSTAVVAKSSRTRKDTLGSKTKEELLEMAKTKDIKGRSSMSKSQLVRSLARS
                     K"
     gene            complement(9839..11248)
                     /locus_tag="VH419_15365"
     CDS             complement(9839..11248)
                     /locus_tag="VH419_15365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7045048.1"
                     /translation="MNDEAFDRFVISHAPVIQGFCRLVIGDPDVLRDVTTGGWSEVYR
                     RWPRIVDDDDRLALALRPFVSDAIRRTRADEPAASGGLELREDTLGPAETVLSREPDL
                     DLVGEALRDLTPRQRALVALRWYVGVDEHALSRAIGWWPPRPAQEADRALRDLMLTVA
                     PHLAPPGRVAGSAWQHEELLRHIQADLADRAGVQDGDGQDVLAQLRPHLAGVEQQGRP
                     PATRRRIVLAATAVAAALVVGIAVSTHRPDEPQPPAQAADSGPPPSVPIATPGMQLVG
                     FQTVYTQVPASWSHNRIECGRPAESTVVYPDASDGCRAGSPMAVALPPSSVTFNTPPT
                     SLLPIGPVRTVNQVGEDPVYATVPRHRAGLLEQIVLIPDRDVQMIVRTPDEHVLDDIV
                     DSLQPVPTGYTVVPPCMRLRLRDAIDRLDDAGLRVRITQSSTLSDRFPVPPVTHQSID
                     SGQLVPVGTVVGLGFPSMN"
     gene            11374..11901
                     /locus_tag="VH419_15370"
     CDS             11374..11901
                     /locus_tag="VH419_15370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HEY7045049.1"
                     /translation="MTIHSEHPFLPPESERDPVRRWRGRLGGTVSLWTSGTGTGPDGQ
                     AGLTVSSYLVAAGEPGHVLALLDPDALLTDRMLQTRTAVVQLLEWKHRDLADAFAGVA
                     PAPGGVFTLGRWVDTEWGPRLADASSWAGVRLSTGEAKTVGWSVLVDTVIEHLEIGEE
                     HQRLVHHRGRYLRPT"
     gene            11969..12913
                     /locus_tag="VH419_15375"
     CDS             11969..12913
                     /locus_tag="VH419_15375"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEY7045050.1"
                     /translation="MDWFGAALKPMPGGYSGETFLVGDESDEQFVVRIYRRDPGRAPV
                     DASLLRLMRGIVPVADVVEARPADNDAPAILVTRRIDGVPLDVVLREDLDGIDWEQLG
                     LDVGAILARLSGVPFLQGGMFEDAALGLAPAPLPDDLTEWARHFRDDGRLAAWQQRDF
                     DALLHLTDAAEDISAEAAEEDGGRVVLVHSDFNPKNILVDPDEGHVVGLVDWEFAYAG
                     SPYADYGNLSRFEREDRLVGPAIEAFSDLAPRVRDIDGRARAADLWALIELAGGVPSN
                     PVRELASELLLAQARTGNLHAWPWKTPRVDPNSAKPVP"
     gene            13090..>13465
                     /locus_tag="VH419_15380"
     CDS             13090..>13465
                     /locus_tag="VH419_15380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020388850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEY7045051.1"
                     /translation="MTSSIEAPPAAQETPQVAVNDVGTAEDFLAAIDATIKYFNDGDI
                     VDGTIVKVDRDEVLLDIGYKTEGVIPSRELSIKHDVDPHDVVEVGDKVEALVLQKEDK
                     EGRLILSKKRAQYERAWGTIEKI"
BASE COUNT         2037 a         4521 c         4703 g         2204 t
ORIGIN      
        1 cccgaacttc aacgccctga actgcgacca gaaccccgct gatgatccca atccagtcga
       61 cgactgcggg atcgggcagg tgcagcaact ggtcggggca gggatcgacc agctggtgct
      121 ctcgatctcc gatcgactca gcggcgtact cggccaggcc tacaccggtt cgcaggacct
      181 gtacgcaggc acgcagcagc tggcccacgg cacccgcaag ctggctgacg gcagcacgaa
      241 gctggtcgac ggtacggtac agcttgccga cgggaccgtg gtcctggctg acggcacccg
      301 ccagcttgcc gacgggaccg tcgtcctggc tgacggcacc cgccagcttg ccgacgggac
      361 cgtggtcctg gcagacggga cccggctcct gaagaacggc gcggtccggc tccacaacgg
      421 tgcgggcaag ctcgacgacg gcggtgtgaa gctgtccgac ggagcagacc agctctccaa
      481 cggggcgact cagctcgatg acggcagcca tcagctgctg agtggtttga ccaccgccgc
      541 ggacggcagc aaccgcctgc gtgctggtct gcagaaggcc gccgacgccg cgccccagct
      601 gtccgacggt gccacgaagc tgtcgaagca gggcaccgag aagctgatcg aggcagggtc
      661 agacacgacg gtggagttcg gtgcgaagta cgcccagatc caggccggtg ccgagcgggc
      721 tcacgacgag agcatggcgt tcggggctcc gcagggtgcc gacgggctga cggcgtacac
      781 gtacgagatc cgaggcgagg acggcgaggg ctcccgcaac tggacccgcg ggatcgcagc
      841 gatcgccctc ttcggtctgg gtgccggcgc gtacgcgctc cgacgccggt tcgtgtgacg
      901 tgaccgctgg ttgagccgcc gacgagcggc tcaaccagcg ggcgcgcgag tcacgaacaa
      961 ggcggttccg ggtagcagtg agccgcggac cggtccccac ccaccccaca cccgcgtgtg
     1021 gccgggtggc cactcgggct cgacgagcga ctccacgtcg agtccgctgg cggtgatcgc
     1081 ttcgatccag tcgcccagcg tgtggtgcgg ctcgacgtac gtcgctccgc cgtcgtgagc
     1141 cacctcgacg tatgcgctgc ggtcgaagta cgacctgacg atcgtcatac cggcgctggt
     1201 gggctcgtcg gggaacatcc ggcgtaccgg atgcacgact gagaacgcga agagaccgtc
     1261 tgctcgtaac acccggacca cttcgcggaa cgctcgctcg atgtccacga cgaacggcag
     1321 cgcaccgaac gccgaacaga cggcgtcgaa cgagtcgtca ccgaacggca gcgctgtcgc
     1381 cgagccgcag acgaccggga cgttcacgcc ggtcgcgtcg tccagccgat ggccgtgctg
     1441 gagcatgcgc tgggagaggt cgacgccgac ggcggcggcg tgcttggtgc gcagccatcg
     1501 ggcgcactgg gcggccccgc agccgaggtc gaggacgcgc cggcccgaga catcccccag
     1561 cagacgagcg tcctgctcgt ccagaccctc cggacaccac acgaagcgtg cgtcaccgag
     1621 gaacgtgccg tgctcacgtt ggtagctgtc cgcctccgcg tcccagtccg cacggttcgc
     1681 cgtctgcgcc tccgcgtcgc tgacatggcg gaactcgctg cgcatgctcg aactctaggg
     1741 gcgcggaggc gcgcggcggg tggaccaagc catgccacct cgtcccgtgt gcacgtggtc
     1801 tgcgactaac ggtcctcttg cctgacattt gtaatgggga agtcgtgatg gtcgacctaa
     1861 cgaccaggtc ttgtgtcgta cgacgatcga gacatgaatc tttcgcactc gaggcaggcc
     1921 gggccgccgg ccgtggcatt ggacgacgtg agcagggtct tcggtgccgt acgggccgtc
     1981 gacggactca gtctcacgat ccaacctggt gagatcgtcg cgttgctggg acccaacggc
     2041 gcgggcaaga cgacgacgat cgacatgatt ctcggcctgt cgacgccgac ccgcggagat
     2101 gtcagtgtct acggcttgcg gccggtcgac gcgatggtgc gtggtctggt gtcggccgtg
     2161 atgcagagcg gcggtctgct gaaggactac acggtggagg agaccgtgca gctcaccgca
     2221 tcactgttcc cgtccagccg tccggtgtcg gaggtcatgc agcgggctgg actcgagcac
     2281 atcgccggcc gacgggtcgg cgcgtgctcg ggcggcgagc aacagcggct gcggttcgcc
     2341 ttggcactgc ttcccgaccc ggagctgttg atcctggacg agccgaccac agggatggac
     2401 gtgacgacgc gtcgcgagtt ctggtcggcc atccgccgcg acgcccaccg cggccgcacg
     2461 gtcatcttcg ccacccacta cctggaggag gccgatgcgt acgccgaccg ggtcgtgctg
     2521 gtccggtcgg gtcgactcgt cgccgacgga accgcgagcc agatcaaagg actcgcctcc
     2581 ggacgtacgg tccgggccac cattccggaa tccggcgacc acgcggacgc ggtgctccgc
     2641 tcactccccg gggtcgacct cgtcgagcga cgcggcgaca ccgtcttcgt ccatggctcc
     2701 gacagcgacg ctgtggcgcg actgctgctg acgtcgacgg atgcccggga cctggagatc
     2761 acctctcgca acctcgaaga cgcgttcgtt gcgctgacca gcgacgacct cgaaggagtc
     2821 ccttcatgac caccgacatg gacacccaca tgggtcccga gacgagcacg ctcgccaccg
     2881 ccgtcggcag catcgccacg tcacagcggc cgacaccggc acttggcggg ttctcgctga
     2941 cgttcgtccg gctcgagcta cggcggatgc tgcgcaaccg gcgtacggtc atcttcacgt
     3001 tgatcatgcc gagcatgttc ttcctgctct tcgggacgga ctcgagccag cagcacgaca
     3061 gcgtcggtcg aggcaacgtg acggcgtacg tcttgatcag catggccgtg tacggcgcga
     3121 tgctggcgac gacgagcggg ggagcctcgg tctcactcga acgcgccgtc ggctggagcc
     3181 gccagctgcg actcacgccg ctccaaccgg tcgcgtacat cgccgtcaag gtcgtagtcg
     3241 ccatgtcact cggtctggca tcggtggtcg tcgtgagcgt gacgggatcg ttcttcggcc
     3301 cgcagcttcc gctccacgtg tgggtcatga gcgcagcgct cgcctggatc tgctccgtcg
     3361 tgttcgccgc gttcgggttg tgcatgggtt atctgctgcc gagcgagaac gtcatgcagg
     3421 ttctcggtcc ggcgcttgcc ctcctgtcgt tcgccggagg gctgttcgtc ccgctcgacc
     3481 agatgggcca cacgttctcc acgctcgcca cgatcacccc ggtgtacggc gtcggcgtgg
     3541 tcgcgcgtta cccgttgacc agcgacggat cactcccgat cgcggtcatc aacgtgctgg
     3601 cgtggacctg cgtcttcgtg gtcggcgcgg cctggcggtt ccgtcgagac accgcgagag
     3661 tctgacgtgg ctctgcaggt gttccggctc gtggcctgga tgtggtcgcg atggcgtcga
     3721 cgccaggttg tccggggctg atcgcgtaat agcgtcgtgt ccatggcagg caacccgacg
     3781 accgtcccgg ggatctccgt ccccggggct ccggtcgaca cgtcgtttcg gtggcggttc
     3841 ggctggatct tcgcggctgc atggctggtc tacctgggtc agccattgtc ggagatggtc
     3901 catgactcgc caggctggcg gcagttcctc gggttcgcgt cgctggccgt gttcgtcgcc
     3961 ctgttccttg cggcggtcgt ccagggacgc agggcaagac gcggcctgag cgtcgagctg
     4021 ccggtctgga agcggcttgc cgtggtcgcc ggcctcctgg tgtgcgtggt ggggatggtc
     4081 cccgctgcag gagctgccgc gttgacggga gtgacgttcg ccgccgcagt ggccgcgatg
     4141 acgctgccgg tgcggcaggg cctcgtcgtc gtactcgcgc tcttcgcgtc gaccgagctg
     4201 ttggtgcggg tcgtgccggg atgggcggac aaggggtacg ggctcagcgt catccttgcc
     4261 ggcgtcgcag cgggcgcctt tcgcgccgcg ctggagcgca acgccgcact gctgcgggcg
     4321 cagcaggagc tcgctgacct ggcggtcgag gaagagcgct cgcgcatcgc cagggacctg
     4381 cacgacatcc tcggtcactc gttgaccgtg atcacggtca agtccgagct ggcacagcgg
     4441 ttgctcgacg tcgacacgga gcgtgcccgg gcggagctgg ccgacctgga ggagctgtgc
     4501 cgggatgcgt tggccgacgt gcgcgcgacg gcactcggcg tacgaggcgt gtccctggcc
     4561 ggcgagatcg ccaccgcacg gacggcactc gagtcggccg ggatcgaggc gacgctgccc
     4621 gtcgcggcgg acgcggtgtc gagccgctgg cgcgaggtgt tcgcgtggac gatcagggaa
     4681 ggtgtcacga acgtgatccg acacagccgg gcctgtcact gccgggtcga gatggggtcg
     4741 gaccatgtga gtgtgagcga cgacggcgtg gggatggacc tcgggcgcgt cgcgaccggt
     4801 cagggactca gcggtcttcg ccagcgggtg gaactggctg gggcgaagct gtcgacgggg
     4861 cccggtgagg gcggccgcgg cgtgcgcctg ctcgtcgagg tgccggcgtg atgacgcaga
     4921 tccatgacac cgccgagacc ggcgtgatcc gggtgttgct cgccgacgac caagggctcg
     4981 ttcgcggtgc ggtggcggca ttgctcgacc tcgaacccga cctcgaggtg gtggcggacg
     5041 tgggggccgg cgacgaggtg gtggacgccg cgttggcggc ccgtccggac gtcgccctgc
     5101 tcgacgtcga gatgccggga ctggacggcg ttgctgctgc gcgtcgtctg cgcacggcgc
     5161 tgccgtcgtg ccggatcctg atggtgacga cgttcggccg gccgggatac ctgcggcagg
     5221 ccatggcggc cggcgcgtcc ggcttcgtcg tcaaggacac gccggcgcgg cagctcgctg
     5281 acgccgtacg ccgcgtccac gaagggctgc gcgtggtcga cccggcgctg gcggcggact
     5341 cgctcacgta cggagagtcg ccgctgacgg aacgggagcg cgacgtcttg cgggcggcgt
     5401 cggacggcgg aaccgtggcg gacatcgcgg cgacgctgca cctgtcggac gggacggtgc
     5461 gcaaccacct ctccagcgcc atcgggaaga ccggagcccg gactcgcgcc gaagccgctc
     5521 gcgtcgccgc cgacaacggc tggctcctct gaccctccgt cgaggtttgc gatggaaaca
     5581 tcccgcctgg actgtttgca tcgcggatcc tcgatgtcca cacgccttct gggctgtaaa
     5641 catcgcaaac ctcgaacgcc tcagctgagg gcggcggtga gggtggaacc ggcgggggcg
     5701 gagaccttga gggtcaggag gtggtcggcc cgggtccggc cgaggttcac cgcggcgacc
     5761 gggatgccac ggcggtgcgc ggcgagcgcg tatcggtagc cggagtgcac catcaacgac
     5821 gaccccacga ccagcatcgc gtcggaggtg accagcgccg ccgtcgcctg cgtatgccgc
     5881 cacggcggga cattctcacc gaagaacacc acgtccggct tgataacacc gccacagcgc
     5941 gggcaagcgg gtacgacgaa ccgcgagaag tcgagaccgt ccagatccgc atcgccgtcc
     6001 ggtgcgaccg gggcggagag cccggaccag tcaccgttga ggtcctcgag cctggcctgc
     6061 cacgtcgccc gcgctgccat ctgacgacag gacagacaag tcacccggtc cagtcgaccg
     6121 tgcaggtcga cgacccgcct cgacccggca gcctggtgga gtccgtcgac gttctgggtc
     6181 accagcaggc cgacggcacc ggtccgttcc atcgcggcca gtgctcggtg cgcgtcgttg
     6241 ggagcggctc ggccgatggc gcgccagccg atcaggctgc gagcccagta gcgggaccga
     6301 gtcaatgggt cgcggacgaa cggcaggtac tcgaccggcc gttgtcgttt ccattcgcca
     6361 tcgagtccgc ggtagtcggg gatgcccgac tccgtgctgc agcccgcccc ggtgaggacg
     6421 aagaccttgc gctgccgggc caggaacttc cgcaacgcgc tcgcgtcacc ggccggcggt
     6481 tcggcgaccg gtgacgccag ccgtcgccgc accggatcgc cgacgagccc catggccctc
     6541 gagcgtaccc gcggcgggcc gacgggtcgt cggccaagat gctgagccgt cacaatgagg
     6601 tcatgggcga agccaagatc gggtacgccg cagcccttga gcagttcggc ccgggggaga
     6661 tcctcgacct gtgtgccagt gcggagaagc acgggttctc cggtgtgatg gcggccgacc
     6721 acttccagcc gtggataccg cagcaaggcc aggcgtcgta cgtctggaac gtcctcggtg
     6781 ccctcgggca atgcaccacg ggcgacctcg gacccggcgt cacctgcccg acgttccgac
     6841 accacccggc gatcgtggct caggcggcgt cgacgctgga ggcgatgtat cccggacgga
     6901 cctggctcgg tctcggctcc ggcgaggcgc tgaacgagca cgtcttcggc ggctactggc
     6961 cggaggcgcc ggagcgcatc gcgatgatgt tcgaggcgat cgaggtcatc cagaagctct
     7021 tcaccggtga ggacgtcaag cacaagggcg agtacttccg gatgcacacc acccggttgt
     7081 ggaccagggc agccgaaccg ccgcccatct acgtcgccac tgccggtccg atcaccgccc
     7141 ggcgggcggg agcacgctgc gacggtctca tcacgcccgg cgcgacgacg gagaaggtcg
     7201 ccggcgtgtt ggccaggttc gacgaagggg cgcgccgtgc cgacaaggac cccacggcgc
     7261 tgccgaagct gctgcagctc cacctctcct gggctccgac cgacgagcag gccatggaca
     7321 acgcggtcac cgagtggccg aacgggggga tgaggttcgg caagcaggac gtccggtcgc
     7381 cgtacgactt cgccgccatg gcagcactcg tacgccgtga ggacttcgag ggccggatgg
     7441 tgatctcgtc cgacccggac gcccaccgac aggagatcca acgcttcctg gacctcggga
     7501 tcacccaggt ctacctgcac aacgtcgggc gcaaccagac cgagtggatg gacgtcttcg
     7561 gccgagaggt ccttcccaag ctaaccgcct gatcgacccg cgttgctggt gggttgcctg
     7621 gctacccgtg caaacgcatc ggtagccacg gaaatctagg gtctcctcgg ctttcggtgg
     7681 gcggagatgg tcgcggtggg tgggcgtggt cgccgggctt ccgtcgtgat gggctcaggt
     7741 gggtcggcag ttacgagcgg gaagtgttgc tgcctgctga gctggccggc cgggctcctg
     7801 tcccttgatg gttcggcgtc tgactggcgg gttccggaca tccgtggtcg cccggcgtga
     7861 tggttgcgaa gttcagacgg caaacccact gtgcccatcg gaggtttgcg atgtttacac
     7921 gccttttggg ctgtaaacat cgcaaacccc gaagctggcc ggtccctgaa ggagccccgg
     7981 atttgcctgg catgcctcgt gcacgtggca acacgcccga cgacaacgac ccccggagtc
     8041 accgggtttc tgagcccccc aactgacgca gctcctttga agactcctcg agaacatgcg
     8101 cggcctgggc aagccggttc ccggggtgat ctgtggtggc cgaccgacgg ccggtcaccg
     8161 gcgtcgtggc cgctggaacg accgtcttgg ccggagtcgc tccagtggcc gatcgatctg
     8221 gcgttccgtt cgcgccctgc agctgcctca cctgcgcggg cgagaccagc acgacacctt
     8281 cgagtgtcag cagcttcatg ccgagcagcc gggcaagcac cgacgtctcg atcgcgaagc
     8341 caccggctgc gctgtcagct ggcgcgagat gcgctggctg accggtcggt gcggtcacca
     8401 gcggacgatc gctcgccgcc gttgccgtac gtctccagcc gccgtgacgc cttctccgca
     8461 atctccaaga tctgtgcata ccgctcacgc cctgccttcg taccgaacac gtaaccgagt
     8521 ccgaaggcag cgatcgtcac ggccttcatt ccgagcccca cctcgcacgg gccttggaga
     8581 ccgcggcact cgccagggac aaccccgcga ccgcacccag cgcggtgagt cccgacttcg
     8641 ccgctcgcgg cgcgacgtcg gacaccggtc cgacgagccg ctcccagggg ctcttcttcc
     8701 tgaacaggtt catcactgtg ctcctagtgc tcctactggt cctggtggtc ctggcggtct
     8761 acttcgatcg ggcgagagat ctgacgagtt gtgacttcga catggacgag cgccccttga
     8821 tgtccttcgt cttggccatc tcgagcagct cttccttcgt cttggagccg agcgtgtcct
     8881 tgcgggttcg cgacgacttc gccacgacgg cggtcgaagt cttggtggcg cgggaccttc
     8941 cgttgcggtt cgccaccgct cgttcgctcg accggcggtc ggtcttgacc gtcggcgaca
     9001 accgcttgcg ttcctccttc gccgcgcggg cgaccgcttc cgcggcggac gtggcctcct
     9061 cggcgagccg gcgggcttcg gccagctgct cggtggctgt cgcgacctca cggtccgcca
     9121 gctcctgcgc gcgttcggct tccgaccggg cttgcgccat acgctcgtcg gcctgggcct
     9181 gctgtcgtgc gatcgccgcg tcggcttcct gctgggctgc cgtacgcacg gcgtcgatct
     9241 ccgactcggc cgcgcgccgg gcttcgtcga cacggaggct gacgttctcg gcctgggtac
     9301 gtcgtacctc ggccaggtgc gccttctcct cctccgctgt ggctcgggcc actgcgacgc
     9361 gctggctggc ttcctcggct gagcgcagcg cccggtcctc ggcctcgcgc gcccgctcca
     9421 tggcttctcg ggcctgggcc aaccggtgct cgatcgatgt gttcgaggtc ggcatgacgt
     9481 ccttgatgct ggaccctgcc tcgcggacga gtccgcccag gctctgcgcg gctcggccgg
     9541 tggcatcggc ggtggaagcg cctgcggtca ccgctttgtc gcgggcgtcg tgggccgggc
     9601 tcagtgcctt cgccagcagc cagcttgcgt tgcgcggcag gtccttgacg ccgagcgcca
     9661 tcaaggacgc gaccttctgc ggcgcctgct gggtggtctg attcgaggcc tgcttggcct
     9721 gcttggtggc ccgcttcgtg gcctttttgg gtcggttggc ggtcatcacg tctccttccg
     9781 gtgccctgga gaccgccctc agggccccct gcttccctga tgcgtacccc gtatgcggtc
     9841 agttcatgct ggggaagccg aggccgacca cggtcccgac cggaacgagc tgaccggagt
     9901 cgatcgactg atgcgtcacc ggcggtaccg ggaagcggtc cgaaagcgtc gacgactggg
     9961 tgattcgcac ccgcagacca gcgtcgtcga gtcggtcgat cgcatcgcgc agccgcagtc
    10021 gcatgcatgg tggcacgacg gtgtaccccg tagggactgg ctgcaacgag tcgacgatgt
    10081 cgtccagtac gtgctcatcg ggcgttcgca cgatcatctg gacgtcgcgg tcggggatca
    10141 gcacgatctg ctccaggagc cccgcgcgat gtcgcggaac ggtggcgtag acaggatcct
    10201 cgcccacctg gttcaccgtt cggacgggac cgatcggcaa caggctggtg ggcggcgtgt
    10261 tgaacgtcac gctggacggc ggcagcgcga cggccatcgg cgaaccggca cggcacccgt
    10321 cgctggcgtc cggatagacg acggtgctct cggccggccg accacattcg atccggttgt
    10381 gtgaccacga ggccgggacc tgggtgtaga cggtctggaa gcccaccagc tgcatgcccg
    10441 gcgtcgcgat cggcaccgac ggtggtgggc cagagtccgc agcctgcgcc ggcggttgcg
    10501 gctcgtccgg cctgtgtgtc gacacggcga tcccgacgac gagcgcggca gcaaccgcgg
    10561 ttgccgccag tacgatccgc cgacgtgttg ccggaggtcg accctgctgc tcgacccccg
    10621 ccagatgcgg tcgcagctgc gccagtacgt cttgtccgtc gccatcctgc acgccggcac
    10681 ggtcagcgag atcggcctgg atgtgtcgca acagctcttc gtgctgccag gcagagcccg
    10741 caactcgacc cgggggtgcc aggtgtggag cgaccgtcag catcaggtcg cgtagtgcgc
    10801 ggtcggcctc ctgcgcgggt cgtggtggcc accacccgat cgcgcgggac aaggcgtgct
    10861 cgtccacgcc tacgtaccac cgcagcgcca ccagcgcccg ctgccggggc gtgaggtcgc
    10921 gcagcgcttc gccgaccagg tccaggtcgg gttcgcggct gagcacggtc tcggccggcc
    10981 cgagcgtgtc ctcgcgaagt tccaggccac ccgacgcagc gggctcgtcg gcgcgggttc
    11041 gacggattgc gtccgatacg aacgggcgga gtgcgagcgc gagacggtcg tcgtcgtcga
    11101 cgatgcgcgg ccaacgccga tacacctccg accagccgcc ggtggtcacg tcgcgcagga
    11161 catccggatc accgatgacg agacggcaga acccttggat caccggtgcg tgcgagatga
    11221 cgaagcggtc gaaggcctcg tcgttcacgg tcgtctcgtc caggtcgtcc ccacccgcgc
    11281 cagaccttgg cggagcttgg ttcgcaagcc gggccgacgg cggcggtcgg ccgtgcgctg
    11341 ctcctaccag cctatgcgcc ctaggctcgg gccgtgacga tccattccga gcatccgttc
    11401 ctgccgccgg agagcgagcg tgacccggta cgccggtggc gcggccggct cggagggacc
    11461 gtctcgttgt ggaccagcgg caccgggacg gggccagacg gtcaagccgg gctcacggtg
    11521 tcgtcgtacc tggtcgcggc cggagaaccc ggacacgtgc tcgcgctgct cgacccggac
    11581 gcgctgctga cggatcgaat gctgcagacc cgcaccgcgg tcgtgcagct gctggagtgg
    11641 aagcaccggg acctcgcaga cgcgttcgcc ggtgtcgcgc cggctccggg cggcgtgttc
    11701 acccttggcc gctgggtcga caccgagtgg ggaccgcgac tcgcggacgc gtcgagttgg
    11761 gccggcgtac ggctgagtac cggcgaggcg aagacggtcg gctggtcggt gctcgtcgac
    11821 accgtcatcg agcatctcga gatcggcgag gaacaccagc gcctcgtgca tcaccgcggc
    11881 cgttacctgc gccccacatg accgagttgt cagaagctca tcgacctatg gccccatggg
    11941 attctgacaa ctcggcggag gagaacgaat ggattggttc ggggctgcgc tgaagccgat
    12001 gccgggtggg tacagcggcg agaccttcct ggtcggtgac gagagcgacg agcagttcgt
    12061 ggtgcgcatc taccggcgcg atcccgggcg ggcgccggtc gatgcgagcc tgcttcgcct
    12121 gatgcgcggc atcgtcccgg tcgcggacgt cgtggaggca cgacctgccg acaatgacgc
    12181 cccagcaatc ctcgtcacac gccggatcga cggcgtaccg ctcgacgtgg tgctgcgaga
    12241 agacctcgat ggcatcgact gggagcagct cggtctcgac gtcggagcga tcctggctcg
    12301 actgtccggt gtgccgttcc tccagggcgg gatgttcgaa gatgccgccc tcgggttggc
    12361 tcccgcgccg ctgccggatg acctcacgga gtgggcgcga cacttccgag acgacggacg
    12421 gttggccgcc tggcagcagc gtgacttcga cgcgctgctg cacctcaccg acgcggccga
    12481 ggacatctcg gcggaggcgg ctgaggagga cggaggacgt gtggtcttgg tccacagcga
    12541 cttcaacccc aagaacatcc tggtcgaccc cgacgagggg catgtcgtcg gactggtcga
    12601 ctgggagttc gcgtacgcgg gttcgccgta cgccgactac ggcaacctca gccggttcga
    12661 gcgcgaggac cggctcgttg gaccagcgat cgaggccttc agcgaccttg cgcctcgtgt
    12721 tcgcgacatc gacggcaggg cgagggcggc cgacctgtgg gcgctcatcg aactggccgg
    12781 cggcgtaccg tcgaatccgg tgcgcgagct ggcgagcgag ctgctgttgg cgcaggcgag
    12841 gacgggcaac ctgcacgcgt ggccgtggaa gacccctcgc gttgacccca acagcgcaaa
    12901 gcccgtaccc tagacgatgc gcttttggcc tgcatgtcct cagaccgagc aggccgtgct
    12961 cgccatttca ttcgtgaggg ccatattccc cggcgcttga cgtcgcaggg ttcctcggtg
    13021 ggaacgcgat ccaagggcca aagggcgcgc ccgcacgagt tctatccacc ccatcggagt
    13081 ccctcctata tgacaagcag catcgaagca ccgcccgcgg ctcaagagac gccgcaggtc
    13141 gcggtcaacg acgtcggaac ggcggaggat ttcctcgccg cgatcgacgc caccatcaag
    13201 tacttcaacg acggcgacat cgtcgacggc acgatcgtca aggtcgaccg cgacgaggtc
    13261 ctgctcgaca tcggctacaa gaccgagggc gtcatcccct cgcgtgagct gtccatcaag
    13321 cacgacgtgg acccgcacga cgtggtcgag gtcggcgaca aggtcgaggc cctggttctc
    13381 cagaaggagg acaaggaagg ccggctgatc ctctccaaga agcgcgccca gtacgaacgc
    13441 gcctggggca cgatcgagaa gatca
//
LOCUS       DATBFZ010000502        13158 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_6126794, whole genome shotgun sequence.
ACCESSION   DATBFZ010000502 DATBFZ010000000
VERSION     DATBFZ010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 13158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13158
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6126794"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1025
                     /gene="tuf"
                     /locus_tag="VH419_15385"
     CDS             <1..1025
                     /gene="tuf"
                     /locus_tag="VH419_15385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005055596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEY7045052.1"
                     /translation="EKQRGITISIAHIEYQTESRHYAHVDCPGHADYIKNMITGAAQM
                     DGAILVVAATDGPMPQTREHVLLARQVGVPAIVVALNKCDMVEDEELIELVELEVREL
                     LSEYEFPGDDTPVVRVAAFPALNGDEKWQQSVLELMDAVDTYIPQPERDTDKPFLMPV
                     EDVFTITGRGTVVTGRIERGVIHINETVDIIGIHDEKQSTTVTGIEMFRKLLDTGEAG
                     ENVGLLLRGTKREEVERGMVVIKPGSTTPHTNFEANVYVLSKEEGGRHTPFFNNYRPQ
                     FYFRTTDVTGVVTLPEGTEMVMPGDNTEMSVELIQPIAMEDGLRFAIREGGRTVGAGR
                     VTKIVK"
     gene            1134..1661
                     /locus_tag="VH419_15390"
     CDS             1134..1661
                     /locus_tag="VH419_15390"
                     /inference="COORDINATES: protein motif:HMM:NF039409.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6328 family protein"
                     /protein_id="HEY7045053.1"
                     /translation="MNSPERRNGGGRSTDRPNESEAERLDRNYGELLQEMRVLQAGVQ
                     ILFAFLLTVAFQSGFSGVDTFQRTTYVLALVAAALAAALIIGPVPFHRFVFRRGMKAD
                     LIKAATRYVAAGLVFLFAAMIGAVMLVLDFLVSRTLAYVVSGLIALVFIALWLVVPVS
                     ARAQARAATQDEPGD"
     gene            1712..2206
                     /locus_tag="VH419_15395"
     CDS             1712..2206
                     /locus_tag="VH419_15395"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY7045054.1"
                     /translation="MSSDRPLGAELLSVIARLNRWATRQADLSLPPAQGRLLAQIDEL
                     GTARIGDLARADHCSQPTMTTQVQRLEERGWVSREQDPADARAVLISLTEQGRELLAY
                     VRRRRAQVLAPYLDDLDDTERARIEDAVDVLRSFLDAVSAAARPTFLPAPETAPDSGA
                     QEDN"
     gene            2209..3426
                     /locus_tag="VH419_15400"
     CDS             2209..3426
                     /locus_tag="VH419_15400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY7045055.1"
                     /translation="MWRQPKAVWAVGFACVIAFMGIGLVDPILKPIADDLGASPSQVS
                     LLFTSYMAVMGVAMLITGWIASRIGAKKTLLLGLLVIIVGAGLAGMQDTVSGIVAFRA
                     VWGLGNALFIATALATIVNAARGSVAQAIILYEAALGLGIAAGPLIGGELGSISWRGP
                     FFGVSVLMTVALVATALFLEATPPAARPTSIREPFRALRHRGLLVVAITALLYNFGFF
                     TLLAFTPFPLDMTAHQVGLVFFGWGLALAFTSVVVAPRLQRRFGTIPSVVVSLLALSV
                     ILAVMAKWTDDRGLLAACVVIAGLFLGINNTLVTETVMKAAPVERGVASAAYSFVRFS
                     GGAIAPYLAGRLGEEFNVHVPFWVGAVAVLAAAFVLAGGRRHLAAVDADVALGTSVED
                     SEEEAVFVTVGDA"
     gene            complement(3444..3803)
                     /locus_tag="VH419_15405"
     CDS             complement(3444..3803)
                     /locus_tag="VH419_15405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020579170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY7045056.1"
                     /translation="MRITRTVIVLDAADLDAVSSFWAGLLGGTVATYDDWHMVEVDGE
                     ARLGIQLAPDHVPPDWPDGAPQQIHLDLWVDDITAAHKEVAALGAKVLKTADLTADDG
                     YQVYADPAGHPFCLCWG"
     gene            3858..4217
                     /locus_tag="VH419_15410"
     CDS             3858..4217
                     /locus_tag="VH419_15410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7045057.1"
                     /translation="MSLDPVVTNPEHYSVIFENERVRVLEYTDQPGDHTTPHRHPDSV
                     MYTLSAFRRRLVSDGARRDVVLEAGSVGWLPAQQHHGENIGDTPTHVLFVELKEAGRE
                     ADLSPPVDEARLGPQTV"
     gene            4653..4961
                     /gene="rpsJ"
                     /locus_tag="VH419_15415"
     CDS             4653..4961
                     /gene="rpsJ"
                     /locus_tag="VH419_15415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012084982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEY7045058.1"
                     /translation="MAGQKIRIRLKAYDHEVIDSSARKIVDTVTRTGAKVAGPVPLPT
                     EKNVFCVIRSPHKYKDSREHFEMRTHKRLIDIIDPTPKTVDSLMRLELPAGVDIEIKL
                     "
     gene            4975..5652
                     /gene="rplC"
                     /locus_tag="VH419_15420"
     CDS             4975..5652
                     /gene="rplC"
                     /locus_tag="VH419_15420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008360995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEY7045059.1"
                     /translation="MSHHETKTPRKVRGLLGTKLGMTQLWDENNRVVPVTVIEAASNV
                     VTQVRAADTDGYTAIQLGFGDVKPAKVTKPQAGHFSKAGVTPRRHVAEIRTADADTYT
                     LGQEVGADVFTAGELVDVTGTSKGKGFAGVMKRHGFAGVSASHGAHRNHRKPGSIGGC
                     ATPGRVFKGLRMAGRMGVDTVTAQNLTVHSVDAEKGLILVKGAVPGPRGTVVVIRSAA
                     KAKGADQ"
     gene            5649..6365
                     /gene="rplD"
                     /locus_tag="VH419_15425"
     CDS             5649..6365
                     /gene="rplD"
                     /locus_tag="VH419_15425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014847257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEY7045060.1"
                     /translation="MTSIDVIDVKGAKAGSVDLPAEIFDVQVNIPLIHQVVVAQLAAA
                     RQGSADTKNRGEVAGGGRKPYRQKGTGRARQGSVRAPQYAGGGVVHGPTPRSYDQRTP
                     KKMKAAALRGALSDRARDGRLHIVEAIVEGDTPSTKAAVGVLGKLTALPRTLVVLERG
                     DDVAWLSLRNVDTVHVLNVDQLNTYDVLLSDDVVFTKAAYDAFVAGPATGKSVKAVAT
                     STEAEAEQTADEAVEEAEEQ"
     gene            6362..6667
                     /gene="rplW"
                     /locus_tag="VH419_15430"
     CDS             6362..6667
                     /gene="rplW"
                     /locus_tag="VH419_15430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002523451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEY7045061.1"
                     /translation="MSNLYKDHRDVLLAPVVSEKSYALLDQNKYTFLVRKDSNKTEIK
                     IAVENVFNVTVTGVNTINRPGKRRRTRNGWGSRPDTKRAIVSIAEGQRIDIFGGPVS"
     gene            6697..7527
                     /gene="rplB"
                     /locus_tag="VH419_15435"
     CDS             6697..7527
                     /gene="rplB"
                     /locus_tag="VH419_15435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011778573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEY7045062.1"
                     /translation="MPIRKYKPTTPGRRGSSVADFVEITRSTPEKTLVKPLPKKGGRN
                     NQGRITTRHQGGGHKRAYRIIDFRRYDKDGVPAKVAHIEYDPNRTARIALLHYADGEK
                     RYIIAPNGLRQGMQVENGPKADIKPGNNLPLRNIPVGTTVHAIELRPGGGAKIGRSAG
                     VSVQLVAREGNRAQLRMPSGEMRYVDVRCRATVGSVGNAEQSNINWGKAGRMRWKGKR
                     PSVRGVAMNPIDHPHGGGEGKSSGGRHPVSPWGVPEGRTRKRKASDKLIVRRRKTGKR
                     "
     gene            7560..7841
                     /gene="rpsS"
                     /locus_tag="VH419_15440"
     CDS             7560..7841
                     /gene="rpsS"
                     /locus_tag="VH419_15440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015035577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEY7045063.1"
                     /translation="MPRSLKKGPFVDDHLQKKVDAQNDKGTKNVIKTWSRRSMIVPAM
                     IGHTIAVHDGRKHVPVFVTDAMVGHKLGEFAPTRTFRGHEKDDRKSRRR"
     gene            7891..8316
                     /gene="rplV"
                     /locus_tag="VH419_15445"
     CDS             7891..8316
                     /gene="rplV"
                     /locus_tag="VH419_15445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEY7045064.1"
                     /translation="MAVTERRRISARRETLLGDQPGAFAVARFVRVTPMKARRVVDMV
                     RGAQVDDALATLRFAPQAAAADVYKVLESAISNATTGEGLERGTLVVSKAMVDEGPTL
                     KRFRPRAQGRATRILKRTSHITLVVQPGDETATTAGGTR"
     gene            8316..9155
                     /gene="rpsC"
                     /locus_tag="VH419_15450"
     CDS             8316..9155
                     /gene="rpsC"
                     /locus_tag="VH419_15450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010986347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEY7045065.1"
                     /translation="MGQKVNPHGFRLGISTDHKSRWYADKLYKSYVGEDVAIRKLLTK
                     GMDRAGISRVEIERTRDRVSVTIYTARPGIVIGRRGAEADRIRENLEKLTGKQVKLNI
                     REVLNPEIDAQLVAQGVAEQLSSRVAYRRAMRKAIQTAMRSGAKGIKIQCSGRLGGAE
                     MSRSDFYREGRVPLHTLRADVDYGFYEAKTTFGRIGVKVWIFKGEVAGTRAEREAQAA
                     QLAQAGNRNRPGRGRRGDRPERPERGDRYAAEAAPAETTAAAVPAETPAAPAPTETPG
                     QEA"
     gene            9160..9579
                     /gene="rplP"
                     /locus_tag="VH419_15455"
     CDS             9160..9579
                     /gene="rplP"
                     /locus_tag="VH419_15455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEY7045066.1"
                     /translation="MLIPRRVKHRKQHHPKRTGAAKGGTKLAFGDFGIQAVEGHYVTN
                     RQIESARIAMTRHIKRGGKVWINIYPDRPLTKKPAETRMGSGKGSPEWWIANVKPGRV
                     MFELSGVPEQIAREAMRRAQHKLPMKTRFVSREGGDF"
     gene            9576..9869
                     /gene="rpmC"
                     /locus_tag="VH419_15460"
     CDS             9576..9869
                     /gene="rpmC"
                     /locus_tag="VH419_15460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007078628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEY7045067.1"
                     /translation="MMASTTAAELRHLDDEGLEEKLRESKEELFNLRFQAATGQLESH
                     GRLRHVRRDIARIYTIMRERELGITDSASDDEAAASSSNDSNDSNGSNGKGAA"
     gene            9866..10144
                     /gene="rpsQ"
                     /locus_tag="VH419_15465"
     CDS             9866..10144
                     /gene="rpsQ"
                     /locus_tag="VH419_15465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004950457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEY7045068.1"
                     /translation="MSDVTTQPSERNRRKVREGVVLSDKMDKTVVVSVEDRVKHALYG
                     KVLRRTSKLKAHDETNQCGIGDRVMIMETRPLSATKRWRVVQVIEKAK"
     gene            10203..10571
                     /gene="rplN"
                     /locus_tag="VH419_15470"
     CDS             10203..10571
                     /gene="rplN"
                     /locus_tag="VH419_15470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEY7045069.1"
                     /translation="MIQQESRLKVADNTGAKQILCIRVLGGSGRRYAGIGDVIVATVK
                     DAIPGGAVKKGDVVKAVVVRTAKERRRPDGSYIRFDENAAVLLRQDGEPRGTRIFGPV
                     GRELRDKRFMKIISLAPEVL"
     gene            10574..10975
                     /gene="rplX"
                     /locus_tag="VH419_15475"
     CDS             10574..10975
                     /gene="rplX"
                     /locus_tag="VH419_15475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEY7045070.1"
                     /translation="MTHKTHKTHKARNLTIKKGDVVRVVAGKDKGAEGKVIAVEPENN
                     RVIVEGVNRVKRHTRLVQQGGRGGTTGGIITQEAAIHASNVMLLTEVDGKKVPTRIGA
                     KRVEVEKTRTDGTTYTKHRSVRIASKNGEEI"
     gene            10977..11561
                     /gene="rplE"
                     /locus_tag="VH419_15480"
     CDS             10977..11561
                     /gene="rplE"
                     /locus_tag="VH419_15480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017932845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEY7045071.1"
                     /translation="MTDTVTADSPTTGVQPRLKLRYREEIVPALKDEFSYPNIMQVPG
                     LTKIVVNMGVGEAARDSKLIEGAVRDLATITGQKPQVTRARKSIAQFKLREGMPIGAH
                     VTLRGDRMWEFLDRLLSLALPRIRDFRGLSPRQFDGKGNYTFGLVEQAMFHEIDQDRI
                     DRQRGMDITIVTTATNDDEGRSLLKRLGFPFREN"
     gene            11567..11752
                     /locus_tag="VH419_15485"
     CDS             11567..11752
                     /locus_tag="VH419_15485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003948630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEY7045072.1"
                     /translation="MAKTSLIVKAARKPKFKVRGYTRCQRCGRPKAVYRKFGLCRVCL
                     REMAHRGELPGITKISW"
     gene            11998..12411
                     /gene="rpsH"
                     /locus_tag="VH419_15490"
     CDS             11998..12411
                     /gene="rpsH"
                     /locus_tag="VH419_15490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003974254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEY7045073.1"
                     /translation="MTMTDPIADMLTRLRNANQAYHESVSMPYSKLKSGVAEILQQEG
                     YIGSWKVEEPADAATVVGKTLVIDLKYGPNRERSIAGVRRISKPGLRVYAKSTGLPKV
                     LGGLGVAIISTSNGLLTDRQAKQKGVGGEVLAYVW"
     gene            12437..12979
                     /gene="rplF"
                     /locus_tag="VH419_15495"
     CDS             12437..12979
                     /gene="rplF"
                     /locus_tag="VH419_15495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002514854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEY7045074.1"
                     /translation="MSRIGKLPITVPSGVDVALDGQTVTVKGPKGTLSHIVREPIKVA
                     KGDDGSVQVTRPDDERESRSLHGLSRTLIANMVTGVTDGYEKKLEIVGVGYRVVSKGP
                     AQLEFALGYSHSITVDAPEGVAFAVESPTKFAVQGIDKQVVGEVAAKIRKLRKPEPYK
                     GKGIRYAGEQVRRKVGKAGK"
     gene            12979..>13158
                     /locus_tag="VH419_15500"
     CDS             12979..>13158
                     /locus_tag="VH419_15500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012923671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEY7045075.1"
                     /translation="MAIALRTRKHTAAKSSSRQRRQVRGRKKLHGSAQRPRLVVTRST
                     RHLFVQVVDDLAGKTL"
BASE COUNT         2389 a         4446 c         4380 g         1943 t
ORIGIN      
        1 aggagaagca gcgcggcatc acgatctcga tcgcccacat cgagtaccag accgagtccc
       61 gccactacgc ccacgtcgac tgcccgggtc acgccgacta catcaagaac atgatcaccg
      121 gcgccgcgca gatggacggc gcgatcctcg tggttgcggc caccgacggc ccgatgccgc
      181 agacgcgtga gcacgtgctg ctcgcccgcc aggtcggcgt gcccgccatc gtcgtggcgc
      241 tgaacaagtg cgacatggtc gaggacgagg agctcatcga gctcgtggag ctcgaggttc
      301 gtgagttgct gtccgagtac gagttccccg gcgacgacac cccggtcgtg cgcgtggctg
      361 ccttcccggc gctgaacggc gacgagaagt ggcagcagtc ggtcctcgag ctgatggacg
      421 cggtcgacac ctacatcccg cagccggagc gtgacaccga caagccgttc ctcatgcccg
      481 tcgaggacgt cttcacgatc accggtcgtg gcacggtcgt caccggccgg atcgagcgcg
      541 gtgtcatcca catcaacgag accgtcgaca tcatcggcat ccacgacgag aagcagagca
      601 ccacggtcac cgggatcgag atgttccgca agctgctcga caccggtgag gcgggcgaga
      661 acgtcggcct gctcctgcgc ggcaccaagc gcgaggaggt ggagcgcggc atggtcgtca
      721 tcaagccggg ctcgacgacg ccgcacacca acttcgaggc gaacgtctac gtcctgtcca
      781 aggaggaagg tggccggcac acgccgttct tcaacaacta ccgtcctcag ttctacttcc
      841 gcacgacgga cgtgacgggt gtcgtgacgc tgccagaggg caccgagatg gtcatgcccg
      901 gtgacaacac cgagatgtcc gtcgagctga tccagccgat cgccatggaa gacggtctgc
      961 ggttcgccat ccgcgagggt ggtcgcaccg ttggcgccgg ccgcgtcacg aagatcgtca
     1021 agtaacaacg cttcacacgc gtccagacgg gccccggact ccgcaggagt ccggggcccg
     1081 tttcacgtct tcgcccatca gccgaatcga caacggagtg gttgaatccg gggatgaact
     1141 ctccggagcg ccgcaacggt ggcgggcggt caaccgaccg gccgaacgag tcagaggcgg
     1201 agcgtctcga ccgcaactac ggcgagctgc tgcaggagat gcgcgtgttg caggccggcg
     1261 tgcagatcct gttcgcgttc ctgctgacgg tggcgttcca aagcgggttc agtggcgtcg
     1321 acacgttcca gcgcacgacg tacgtgctgg cgctggtggc ggcggccctg gccgcggcgc
     1381 tcatcatcgg gccggtgccg ttccaccgct tcgtcttccg ccgcgggatg aaggcagatc
     1441 tcatcaaggc tgccacccgt tacgtggctg ccggactcgt cttcttgttc gcggcaatga
     1501 tcggtgcggt catgctcgtg ctcgacttcc tcgtcagccg gacgctcgcg tacgtcgtgt
     1561 cggggctgat cgccctcgtc ttcatcgcct tgtggctcgt cgtgcccgtg tccgcacgtg
     1621 ctcaggcccg tgcggccact caagacgagc cgggggactg agctgtccgg atttggagtt
     1681 agataggtct cctatgtaaa ctcgacgggt gatgtcatcc gatcgaccct tgggcgccga
     1741 gctgctcagc gtgatcgccc ggctcaaccg atgggcaacc cggcaggccg acctgagtct
     1801 tccgccggcg cagggccggc tgctcgccca gatcgacgag ctcggcacgg ctcgcatcgg
     1861 cgatcttgcc cgggccgacc actgcagcca gccgacgatg accacgcagg tccagcggct
     1921 cgaggagcgc ggctgggtct cccgcgaaca ggatccggct gacgcgcggg cggtactgat
     1981 ctcgctgacc gagcaggggc gtgagctgct tgcgtacgtt cgtcgccgcc gtgcccaggt
     2041 cctcgcgccg tacctcgacg acctcgacga cacggaacgg gctcggatcg aggacgccgt
     2101 cgacgtactc cggtcgttcc tcgacgccgt gtcggcggct gcccgaccta cctttctgcc
     2161 agcacccgag accgcacccg attcgggtgc acaggaggac aactgaacat gtggcgccaa
     2221 cccaaagccg tctgggccgt cggattcgcc tgcgtgatcg cgttcatggg catcgggctc
     2281 gtggacccga tcctcaagcc gatcgctgac gatctcggcg cctcgccgtc gcaggtgtcg
     2341 ctgctgttca ccagctacat ggccgtgatg ggcgtcgcga tgctcatcac cggctggatc
     2401 gccagccgca tcggcgccaa gaagacgttg ctgctgggcc tgctggtgat catcgtcggc
     2461 gccggcctcg ccggcatgca ggacaccgtc agcggcatcg tggccttccg cgcggtgtgg
     2521 gggctcggca acgcgctgtt catcgccacc gcgctggcca cgatcgtgaa cgcggcacgt
     2581 ggttcggtcg cccaggcgat catcctgtac gaagcggcgc tcggcctcgg catcgccgcc
     2641 ggcccgctca tcggtggtga gctcggcagc atctcctggc gtggtccgtt cttcggcgtc
     2701 tccgtgttga tgacggtcgc gctcgtggcg acggcgctgt tcctcgaggc gacgcctccg
     2761 gccgctcggc cgaccagcat tcgcgagcct ttccgtgcgc tgcgtcatcg cggactgctc
     2821 gtggtggcca tcaccgcact gctgtacaac ttcggcttct tcacgctgtt ggcgttcacg
     2881 ccgttcccgc tcgacatgac cgcgcaccag gtcggcctgg tcttcttcgg ctggggcctg
     2941 gcgctggcgt tcacgtccgt cgtggtggcg cctcggttgc agcgccgctt cgggaccata
     3001 ccgtcggtgg tcgtctcact cttggcgctg tcggtgatct tggccgtgat ggcgaagtgg
     3061 accgacgaca gagggctgtt ggccgcctgc gtcgtcatcg ccggcctgtt cctcggcatc
     3121 aacaacacgc tcgtcaccga gacggtcatg aaggccgctc cggtggagcg cggggtcgcg
     3181 tcggcggcgt acagcttcgt ccgcttctcc ggaggcgcga tcgccccgta cctggctgga
     3241 cggctcgggg aggagttcaa cgtccacgtg ccgttctggg tgggcgcggt ggccgtactc
     3301 gccgctgcgt tcgtgctggc cggcgggcgt cgccatctgg ccgccgtgga cgcggatgtc
     3361 gccttgggta cgtcggtcga ggacagcgaa gaggaagccg tcttcgtcac cgtcggcgac
     3421 gcctgaaggg cacggccccg agttcagccc cagcagagac agaaggggtg cccggccggg
     3481 tcggcgtaca cctggtagcc gtcgtctgcc gtcaggtcgg ccgtcttcag caccttggcg
     3541 ccgagggcag ccacttcctt gtgcgccgct gtgatgtcgt ccacccagag gtcgaggtgg
     3601 atctgttgcg gcgctccgtc aggccagtcc ggcgggacgt ggtcaggcgc cagctggatt
     3661 cccagccgcg cttcgccgtc gacctcgacc atgtgccagt cgtcgtacgt ggcgaccgtg
     3721 ccaccgagca gtccggccca gaatgagctc acggcgtcca ggtcggctgc atcgaggacg
     3781 atcaccgtcc gagtgatccg catggcccca tcctggcaag agcgcggcac gggcgcggcg
     3841 tctgccatcc tgcacggatg agcctcgacc ctgtcgtcac caaccccgag cactactcgg
     3901 tcatcttcga gaacgagcgg gtacgcgtgc tcgagtacac cgaccaaccc ggcgaccata
     3961 cgacgccgca ccggcatccg gacagcgtca tgtacacgct gtccgctttc cgccggcggc
     4021 tggtctccga cggggcgcga cgcgatgtgg tgctggaggc ggggtcggtc ggttggctcc
     4081 cggctcagca gcatcacggg gagaacatcg gcgacacgcc cacgcacgtg ctcttcgtcg
     4141 agctgaagga ggctggacgc gaggcagatc tctccccacc cgtcgacgaa gcgcggctcg
     4201 gcccgcagac cgtctgagga ggggttccgg gcagcgcctc cccgccaaga cttccaccgc
     4261 gagccggtcg cggggacggt ggagaagcct gcgactcaca tcacacccct gatcggtcgc
     4321 ggaggctgat ttggcagcgt cgggggcgat ctggcaaact atcggggttg ctctggcggg
     4381 gtcgccgggg tgcgtgatcg aggtttcgac cgtggtcctg gccacggaca aggccgggtc
     4441 ccaccgcacc ggagacgctg ctcaaggccg acgatctgtg ggtttgctcc agaccgtggc
     4501 cctagacctc cccgtacgac gatcgacgag atctgcggcg tgccctgcgg acaccgcgcg
     4561 acacacccga cctcgggggt cggagtacgg ctcgacgcca accgggcggc gggcaggcac
     4621 atgtcatcga tagtgaagga cgaagcgaga gcatggcggg acaaaagatc cgcatccggc
     4681 tcaaggccta cgaccacgag gtcatcgact cgtcggcgcg caagatcgtc gacacggtga
     4741 cgcgtacggg tgcgaaggtt gcgggcccgg tgccgctgcc taccgagaag aacgtgttct
     4801 gcgtcatccg ctcgccgcac aagtacaagg acagtcgcga gcacttcgag atgcgcaccc
     4861 acaaacgact catcgacatc atcgacccca cgccgaagac ggtcgactcg ctcatgcggc
     4921 tcgagcttcc ggccggtgtc gacatcgaga tcaagctctg aggaaccgac acccatgagc
     4981 caccacgaaa ccaagacgcc acggaaggtc cgcggcctgc tcggcaccaa gctcggcatg
     5041 acccagctct gggacgagaa caaccgcgtc gtccccgtca cggtcatcga agccgcgtcg
     5101 aacgtcgtca cccaggtccg tgctgccgac accgacggct acaccgccat ccagctcggc
     5161 ttcggcgacg tgaagccggc gaaagtgacc aagccgcaag ccggtcactt ctcgaaggcc
     5221 ggcgtgacac cgcgccgcca cgtggccgag atcaggaccg ccgacgccga cacctacacg
     5281 ctgggccagg aggtcggcgc tgacgtgttc accgccggcg agctggtcga cgtcaccggc
     5341 accagcaagg gcaaggggtt cgccggcgtg atgaagcgcc acggcttcgc cggtgtcagc
     5401 gcttcccacg gtgcgcaccg caatcaccgc aagcccggct ccatcggcgg ctgcgccacc
     5461 cccggccggg tgttcaaagg cctgcggatg gccggccgga tgggtgtcga cacggtcacc
     5521 gcacagaacc tcactgtcca ctcggtcgac gccgagaagg gactgatcct cgtcaagggt
     5581 gcggtccccg gcccgcgcgg caccgtcgtc gtcattcgct ccgcggccaa ggcgaaggga
     5641 gcagaccaat gacttcgatc gacgtcatcg acgtgaaggg tgccaaggcc ggcagcgtcg
     5701 acctgcccgc agagatcttc gacgtccagg tcaacattcc gttgatccac caggtagtcg
     5761 tggctcagct cgcggccgcc cgtcagggca gcgccgacac gaagaaccgc ggcgaggtcg
     5821 ccggcggagg ccgcaagccc taccgccaga agggcaccgg ccgcgctcgt cagggctcgg
     5881 tccgcgcacc gcagtacgcc ggcggtggcg tggtccacgg tccgacgcct cgctcgtacg
     5941 accagcggac accgaagaag atgaaggctg ccgcgctgcg tggtgccctg tccgaccgcg
     6001 ctcgcgacgg ccggcttcac atcgtcgaag ccatcgtcga aggtgacacg ccgtcgacga
     6061 aggcggctgt cggcgtactc ggcaagctca cggcgctccc gcgcacgctc gtcgtgctcg
     6121 agcgtggcga cgacgtcgcc tggttgagcc tgcgcaacgt cgacacggtc cacgtgctca
     6181 acgtcgacca gctcaacacg tacgacgtgc tgctcagcga cgacgtggtc ttcaccaagg
     6241 ctgcctacga cgccttcgtg gccggtccgg ccaccggcaa atccgtgaag gccgtcgcga
     6301 cgtccaccga ggcggaagcc gagcagaccg cggacgaggc cgtggaggag gcagaagagc
     6361 aatgagcaac ctctacaagg atcaccgcga cgtcctgctc gccccggtgg tgagcgagaa
     6421 gtcgtacgcg ctgctcgacc agaacaagta cacgttcctg gtccgcaagg actccaacaa
     6481 gaccgagatc aagatcgcgg tcgagaacgt cttcaacgtc acggtcaccg gtgtcaacac
     6541 gatcaaccgc cccggcaagc gtcgccgcac ccgcaacgga tggggcagcc gaccggacac
     6601 caagcgtgcg atcgtcagca tcgccgaggg ccagcgcatc gacatcttcg gaggtccggt
     6661 ctcctgaccg ggctcgagta cgaggactga ggacacatgc ctatccgcaa gtacaagccg
     6721 accacgccgg gccgccgcgg ctcgagcgtc gccgacttcg tcgagatcac gcgctcgact
     6781 ccggagaaga ccctggtcaa gccgctgccg aagaagggtg gtcgcaacaa ccagggccgg
     6841 atcacgaccc gtcatcaagg tggcggccac aagcgcgcgt accggatcat cgacttccgt
     6901 cgctacgaca aggacggtgt gccggcgaag gtcgcccaca tcgagtacga cccgaaccgc
     6961 acggctcgga tcgcgctgct gcactacgcc gacggcgaga agcgctacat catcgcgccg
     7021 aacggactgc gtcagggcat gcaggtcgag aacggtccga aggccgacat caagccgggc
     7081 aacaacctgc cgctgcgcaa catcccggtc ggtacgaccg tgcatgcgat cgagctccgt
     7141 cccggcggcg gcgcgaagat cggccgctcg gccggggtct ccgtgcagct cgttgctcgc
     7201 gagggcaacc gcgcgcagct gcggatgccg tccggcgaga tgcggtacgt cgacgtgcgc
     7261 tgccgcgcca ccgtcggttc cgtcggcaac gccgagcagt ccaacatcaa ctggggcaag
     7321 gccggccgca tgcgctggaa gggcaagcgc ccgagcgtcc gcggtgtcgc catgaacccg
     7381 atcgaccacc cgcacggcgg gggcgagggc aagagctccg gtggacgcca cccggtgtcc
     7441 ccgtggggtg tcccggaggg ccggacgcgc aagcgcaagg cctccgacaa gctgatcgtc
     7501 cgtcgccgca agaccggcaa gcgctgagtc tcgacaggct cgaccaccga aggaagacca
     7561 tgccacgcag cctgaagaag ggcccgttcg tcgacgacca cctgcagaag aaggtggacg
     7621 ctcagaacga caagggcacc aagaacgtca tcaagacctg gtctcgtcgt tcgatgatcg
     7681 tgccggccat gatcggccac acgatcgccg tccacgacgg ccgcaagcac gtccctgtgt
     7741 tcgtgaccga cgccatggtc ggccacaagc tgggcgagtt cgcgccgacg cggaccttcc
     7801 gtggccacga gaaagacgac cgcaagagcc gccgccgctg acgcgggcca ccgactgtga
     7861 ttgccgagaa gccgaaagaa gttactgaca atggctgtta ctgagcgcag gcgcatcagc
     7921 gcccgccgcg agaccctgct gggtgaccag ccgggtgcct tcgctgtcgc gcgcttcgtc
     7981 cgggtgaccc cgatgaaggc tcgtcgcgtg gtcgacatgg tgcgtggtgc acaggtggac
     8041 gacgccctgg ccacgctgcg cttcgcaccg caggccgcgg ccgccgacgt ctacaaggtg
     8101 ctggagagcg ccatctcgaa cgccacgacc ggtgagggcc tggagcgggg caccctcgtg
     8161 gtgtcgaagg cgatggtcga cgagggtccg acgctgaagc ggttccgccc gcgtgcacag
     8221 ggccgcgcga cccgcatcct caagcgcacg agccacatca cgctggtcgt gcagcccggc
     8281 gacgagaccg ctaccacggc aggaggcacc cgctaatggg ccagaaggtc aacccgcacg
     8341 gcttccgtct cggcatctcc acggaccaca agtcccgctg gtacgccgac aagctgtaca
     8401 agtcgtacgt cggtgaggac gtcgcgatcc gcaagctgct gaccaagggc atggaccgcg
     8461 ccggcatcag ccgcgtcgag atcgagcgca cccgtgaccg cgtctcggtg acgatctaca
     8521 ccgcccgccc gggcatcgtg atcggtcggc gtggcgccga ggcggaccgt atccgcgaga
     8581 acctcgagaa gctcaccggc aagcaggtca agctcaacat ccgtgaggtg ctcaaccccg
     8641 agatcgacgc ccagctcgtc gctcaaggcg tcgccgagca gctgtcgagc cgggtcgcct
     8701 accggcgcgc catgcgcaag gcgatccaga ccgcgatgcg gtccggtgcc aaggggatca
     8761 agatccagtg ctcgggtcga ctcggcggcg ccgagatgtc ccgctcggac ttctaccgtg
     8821 agggccgggt tccgctgcac acgttgcgcg ccgacgtcga ctacggcttc tacgaggcca
     8881 agacgacatt cggccggatc ggtgtgaagg tctggatctt caagggtgag gtcgccggta
     8941 cccgcgccga gcgcgaggcc caggcggcgc agctcgccca ggccggcaac cgcaaccgcc
     9001 ccggccgcgg tcgccgcggt gaccgccccg agcggcccga gcgcggtgac cggtacgccg
     9061 ccgaggctgc cccggccgag accacggcgg ctgccgtgcc cgcggagaca ccggctgctc
     9121 cggcgccaac cgagaccccc ggacaggagg cctgacgaca tgctgatccc ccgcagggtg
     9181 aagcaccgca agcagcacca ccccaagcgc accggcgctg ccaagggtgg gaccaagctc
     9241 gctttcggcg acttcggcat ccaggcagtc gaggggcact acgtcaccaa ccggcagatc
     9301 gagtcggcac gtatcgccat gacccgtcac atcaagcgtg gtggaaaggt ctggatcaac
     9361 atctacccgg accgaccact gaccaagaag cccgccgaga cccggatggg ctccggcaag
     9421 ggttcccccg agtggtggat cgccaacgtg aagcccggcc gtgtgatgtt cgagctctcc
     9481 ggtgtccccg agcagattgc tcgcgaggca atgcgccgcg cgcagcacaa gttgccgatg
     9541 aagacgcgtt ttgtgagccg tgaaggcggt gacttctgat ggcatccacg acagcagcgg
     9601 agctgcgtca cctcgacgac gaaggcctcg aggagaagtt gcgcgagtcg aaggaggagc
     9661 tgttcaacct gcggttccag gccgcgaccg ggcagctgga atcccacggc cggcttcgcc
     9721 acgtccgccg cgacatcgcg cggatctaca cgatcatgcg tgagcgcgag ctgggcatca
     9781 cggattccgc ttcggacgac gaggccgcag cgagcagcag caacgacagc aatgacagca
     9841 atggcagcaa cggcaagggt gcagcatgag tgacgtcacg acacagccga gtgagcgcaa
     9901 ccggcgcaag gtgcgcgagg gcgtggtgct gagcgacaag atggacaaga ccgtggtcgt
     9961 gtccgtcgag gaccgcgtca agcacgcgct gtacggcaag gtgctccgtc gtaccagcaa
    10021 gctcaaggcc cacgacgaga ccaaccagtg cggcatcggc gaccgggtca tgatcatgga
    10081 gacccgtccg ctgtccgcca ccaagcggtg gcgggtcgtg caggtcatcg agaaggccaa
    10141 gtaaccagtt ccgcaaggct ctcgccgcgg cgagagaacc ggcagacaac caggagtaag
    10201 agatgatcca gcaggagtcg cgactgaagg tcgccgacaa caccggtgcc aagcagatct
    10261 tgtgcatccg ggtgctcggc ggctccggtc ggcgctacgc cggcatcggg gacgtcatcg
    10321 tcgccactgt gaaggacgcg atcccgggcg gtgcggtgaa gaagggcgac gtcgtgaagg
    10381 cggtcgtcgt gcgcaccgcg aaggagcggc gtcgccccga cggctcctac atccgattcg
    10441 acgagaacgc cgccgtgctg ctccgtcagg acggcgagcc gcgtgggacg cgcatcttcg
    10501 gaccggtcgg ccgggagctg cgcgacaagc ggttcatgaa gatcatctcg ttggctccgg
    10561 aggtgctctg accatgacac acaagacaca caagacacac aaggctcgca acttgaccat
    10621 caagaagggc gacgtcgtcc gcgtcgtcgc cggcaaggac aagggcgctg aaggcaaggt
    10681 catcgcggtc gaacccgaga acaaccgggt catcgtcgag ggtgtcaacc gagtcaagcg
    10741 gcacacgcgt ctggtccagc agggcggccg cggcggcacg accggcggca tcatcacgca
    10801 ggaggccgcg atccacgcct cgaacgtgat gctgctgacc gaggtcgacg gcaagaaggt
    10861 cccgacccgg atcggcgcga agcgcgtcga ggtcgagaag acccggaccg acgggacgac
    10921 gtacaccaag caccgcagcg tgcgcatcgc cagcaagaac ggcgaggaga tctgacatga
    10981 ccgacaccgt gaccgcggac agcccgacga cgggcgtcca gccgcggctc aagctccgct
    11041 accgcgaaga gatcgtgccg gcgctgaagg acgagttcag ctacccgaac atcatgcagg
    11101 tgcccggcct gacgaagatc gtcgtcaaca tgggtgtcgg cgaggcggcg cgtgactcga
    11161 agctcatcga aggtgccgtc cgcgacctgg cgaccatcac cggccagaag ccgcaggtga
    11221 cacgcgcacg caagtcgatc gcccagttca agctgcgcga gggtatgccg atcggcgccc
    11281 acgtgacgct gcgtggcgac cggatgtggg agttcctcga ccgcctgttg agccttgcgc
    11341 tgccccgtat ccgcgacttc cgtggcctgt cgccgcggca gttcgacggc aagggcaact
    11401 acaccttcgg cctggtcgag caggcgatgt tccatgagat cgaccaggac cggatcgacc
    11461 gccagcgcgg catggacatc acgatcgtga cgaccgcgac caacgacgac gagggacgct
    11521 cgctgctcaa gcggctcggc ttccccttcc gggagaactg actgacatgg ccaagacttc
    11581 gctgatcgtc aaggcagcac gcaagcccaa gttcaaggtc cgcggctaca cccgctgcca
    11641 gcggtgtggc cggccgaagg cggtctaccg caagttcggg ctgtgccggg tgtgcctgcg
    11701 cgagatggcg caccgcggcg agctgcccgg catcaccaag atctcctggt aagtcgtgtc
    11761 cacgactcgc ttcgctcttc gtggacgctg tcgacctggg gctccgcccc agaccccgat
    11821 ttcacggttg cggtccccta ccggatcgcc gacgcgcgag aagcgagaac actgaggggc
    11881 tccgccccag accccgattt catggttgcg gctccctacc ggaccgccac ccagagaaaa
    11941 acgaaagacg ctgaaggtcg ggagaccgaa accacagcga gaaagaggcc aacggccatg
    12001 acgatgaccg acccgatcgc agacatgctc acccgtctgc gcaacgccaa ccaggcgtac
    12061 cacgagagcg tcagcatgcc ctacagcaag ctgaagtccg gggtcgccga gatccttcag
    12121 caggaggggt acatcggctc ctggaaggtc gaggagcctg ccgatgcggc cactgtggtc
    12181 ggcaagacct tggtgatcga cctgaagtac ggccccaacc gcgagcgctc catcgccggc
    12241 gtacgccgga tcagcaagcc gggtctgcgc gtgtacgcga agtcgaccgg gctgcccaag
    12301 gtgctcggag ggctcggcgt cgcgatcatc tcgacgtcga acggtctgct gaccgatcgg
    12361 caggcgaagc agaagggcgt aggtggggaa gtcctcgcct acgtctggta gccgaggaac
    12421 tggaggagga agacccatgt cgcgtatcgg caagctcccc atcacggttc cgtccggcgt
    12481 cgacgtcgcg ctcgacggcc agaccgtcac cgtcaagggc ccgaagggca ccttgtcgca
    12541 catcgtccgt gagccgatca aggtcgccaa gggcgacgac ggctccgtgc aggtgacacg
    12601 tcccgacgac gagcgggaga gccgctcgct gcacggcctg tcgcggacgc tgatcgccaa
    12661 catggtcacc ggcgtcaccg acggctacga gaagaagctc gagatcgtcg gcgtcggcta
    12721 ccgcgtcgtg tcgaagggac ccgcgcagct cgagttcgcg ctcgggtaca gccactcgat
    12781 caccgtcgac gcgcccgaag gcgttgcgtt cgctgtcgag agcccgacga agttcgcggt
    12841 ccagggcatc gacaagcagg tcgtcggcga ggtcgccgcg aagatccgca agctccgcaa
    12901 gcccgagccc tacaagggca agggcatccg gtacgccggc gagcaggtcc gccgcaaggt
    12961 cggaaaggct ggtaagtgat ggctatcgca ctcaggaccc gcaagcacac cgcggcgaag
    13021 agctcctcgc ggcagcgtcg ccaggtccgc ggtcgcaaga agctgcacgg ttccgcacag
    13081 cggccgcggc tggtcgtgac ccggtccacc cggcacctgt tcgtccaggt cgtggacgat
    13141 ctggccggca agacgctc
//
LOCUS       DATBFZ010000503         1559 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_6128248, whole genome shotgun sequence.
ACCESSION   DATBFZ010000503 DATBFZ010000000
VERSION     DATBFZ010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6128248"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="VH419_15505"
     CDS             <1..308
                     /locus_tag="VH419_15505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7045076.1"
                     /translation="KDSVATYPRIALAATFSTSILAVGVLVWTDPPVPEFWVVGPRLP
                     LGLGGWPTGSTIILSGLIAWGILIYSLKVYRPQVLEERKAKQDGQPEQNGKAESKAG"
     gene            375..>1559
                     /gene="lpdA"
                     /locus_tag="VH419_15510"
     CDS             375..>1559
                     /gene="lpdA"
                     /locus_tag="VH419_15510"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEY7045077.1"
                     /translation="MSSHYDVVVLGAGPGGYVAAIRAAQLGKKTAIIEKKYWGGVCLN
                     VGCIPSKALLRNAELAHIFTADAKTFGISGDVSFDYQAAFDRSRKVAEGRVKGVHFLM
                     KKNNITEYDGWGTFTDANTLSVDLSDGGEETVTFDNLILATGATTRLLPGTSLSDRVV
                     TYEEQIMTPELPGSVVIAGAGAIGVEFGYVMANYGVDVTIVEFLDRMVPLEDEEVSAE
                     LAKRFKKLGVKVLTGTRVDAIDDSGDSSRPVKVTVTKGDEQQTLEADKVLQAIGFAPR
                     VDGYGLDKAGVELTDRGAIEIDEFMRTNVPHIYAIGDVTAKLMLAHAAEAMAIIAAET
                     IGGAETMEVDFAMIPRATYCQPQIASFGYSEKQARDKGYDINVAKFPFQVNGKANGLG
                     DTV"
BASE COUNT          291 a          499 c          533 g          236 t
ORIGIN      
        1 ccaaggacag tgtggcgacc tatccgcgga tcgcgctggc ggcgacgttc tcgacctcaa
       61 tcctggccgt gggggtcctg gtgtggaccg atccgccggt acccgagttc tgggttgtcg
      121 gaccgcggct acccctcggg ctcggcgggt ggccgaccgg ctccacgatc atcctgtccg
      181 gactgatcgc ctggggaatc ctgatctact cgttgaaggt ctaccgcccg caggtcctcg
      241 aggagcgcaa ggccaagcag gacggccagc cggagcagaa cggcaaggcc gagtccaagg
      301 ccggttgacg tcgcagcacg aaggctcgcc cgactgggtc tgccgagcct tcgtcacttc
      361 aataggctgg acgcatgagc tctcactacg acgtcgtcgt gctcggcgcc ggaccgggcg
      421 ggtacgtcgc cgcgatccgc gccgcacagc tgggcaagaa gaccgcgatc atcgagaaga
      481 agtactgggg cggggtctgt ctcaacgtcg ggtgcatccc gagcaaggcg ctgctccgga
      541 acgccgagct cgcgcacatc ttcaccgcgg acgccaagac gttcggcatc tccggcgacg
      601 tgtcgttcga ctaccaggct gcgttcgacc gcagccgcaa ggtggccgag gggcgggtca
      661 agggcgtcca cttcctgatg aagaagaaca acatcaccga gtacgacggc tggggcacgt
      721 tcaccgatgc caacacgctg tcggtcgacc tctccgacgg cggtgaggag acggtgacgt
      781 tcgacaacct catcctggcg acaggggcga cgacgcgcct gctcccgggg acgtcgctca
      841 gcgaccgggt cgtcacctac gaagagcaga tcatgacgcc cgagctaccc ggctcggtcg
      901 tgattgcggg tgccggggcg atcggagtcg agttcgggta cgtgatggcg aactacggcg
      961 tcgacgtgac gatcgtggag ttcctcgacc ggatggttcc gctggaggac gaagaggtct
     1021 cggccgagct ggccaagcgg ttcaagaagc tcggcgtgaa ggtgctgacc ggcacccgcg
     1081 tcgacgcgat cgacgactcc ggcgacagct cacgaccagt caaggtcact gtcaccaagg
     1141 gcgacgagca gcagaccctc gaggcggaca aggtcctgca ggcgatcggc ttcgcgcccc
     1201 gcgtggacgg ttacggcctc gacaaggccg gcgtcgaact caccgaccgc ggtgcgatcg
     1261 agatcgacga gttcatgcgc acgaacgtgc cgcacatcta cgccatcggc gacgtcacgg
     1321 ccaagctcat gctcgcgcac gcggcggagg cgatggccat catcgcggcc gagacgatcg
     1381 gcggggctga gacgatggag gtcgacttcg cgatgatccc gcgggcgacg tactgccagc
     1441 cgcagatcgc cagcttcggc tacagcgaga agcaggcgcg ggacaagggc tatgacatca
     1501 acgtcgccaa gttcccgttc caggtcaacg gcaaggccaa cggtctgggc gacacggtc
//
LOCUS       DATBFZ010000504         8651 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_4489427, whole genome shotgun sequence.
ACCESSION   DATBFZ010000504 DATBFZ010000000
VERSION     DATBFZ010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 8651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8651
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4489427"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            36..776
                     /gene="larB"
                     /locus_tag="VH419_15515"
     CDS             36..776
                     /gene="larB"
                     /locus_tag="VH419_15515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013861468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HEY7045078.1"
                     /translation="MTDSPDVDDFEATAGVVDASLDLGYARLDVDRVRRTGDPEVVYG
                     AGKSPEQVVDLLDRLRTAHPDRCVFATRLSDDAIAAVRERLPDSEVNETARAVTCGEL
                     PRTRGLVTVVSAGTSDATVTHEAALTARVFGAGTRVINDVGVAGLHRVLGVRAELESA
                     DCLVVVAGMEGALPSVVGGLTGTPLVAVPTSVGYGASFGGVAALLAMLNSCAPGVVVV
                     NIDNGFGAGVFAARVARNAAPKSATLTH"
     gene            794..1963
                     /gene="larC"
                     /locus_tag="VH419_15520"
     CDS             794..1963
                     /gene="larC"
                     /locus_tag="VH419_15520"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020577961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HEY7045079.1"
                     /translation="MIGWIDASSGASGDMLLGALLDAGADEDLVRDSIASVAPEPTTL
                     TLVRARRGSLWGNHALVETAASSSHRGLRDVVGLISSAPLHADVKRHAIDVFNLLGAA
                     EADVHGLTVDEVHFHEVGAVDAIADIVGVCAGFVGLGLSDLHCSPVAVGSGTVTTSHG
                     PLSVPPPAVVHLLHGAPTYAGPAVAELCTPTGAALLAHWVTAWGPQPLMTVDQIGIGC
                     GTRDFDSHPNVLRIFVGVAHEASAAQAVVYETNVDDLDPRLWPTILQRLLDAGASDAW
                     LTPILMKKGRPAYTLSALTNAAAAADVRQVIFAETSAIGLRETYVGKHALDRKMETVE
                     VDGRSIAVKLAVSDGKVVNVQPEYDEVVAAAQDLGRPVKSVLADAAAAAARFWQQ"
     gene            2090..2695
                     /locus_tag="VH419_15525"
     CDS             2090..2695
                     /locus_tag="VH419_15525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017933438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HEY7045080.1"
                     /translation="MDWHLVLISFALIFPVELPDKTFVAALVLSTKYRPLFVWIGVGL
                     AFGVQCLVAVTAGAAVSFLPEWFVHGLSTLMFLLGAIILFRSAPGADAEEREQEESFA
                     EKATREQKGWHAAAASFAVLFAAEWGDLSQILTLNLVAKYGSPLEVFIGSWAALLAVS
                     GLAVIGGRVLLRYMRLSLLHYFAGSVCLIFVVVGIVQLIND"
     gene            2765..3988
                     /locus_tag="VH419_15530"
     CDS             2765..3988
                     /locus_tag="VH419_15530"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020390594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA];
                     GO_process: GO:0019547 - arginine catabolic process to
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEY7045081.1"
                     /translation="MTDAPAPLGADSEVGRLRTVLLHRPGPELKRLTPRNNDSLLFDG
                     IPWVGRAQDEHDAFAAALRDRGVEVLYLVELLVETLADPDARAQAIGHVTASLRLGDT
                     LRAHLGRWLHSLSADELAAAITAGVRNDEVRGGFGLVTALLAHDDFLIDPLPNLLFTR
                     DSSFWLRDRVAVASLAMPARTRETQLMELVYTFHPRFSGVERIHGWRAEHVEGGDVLL
                     LGPGVIAVGVGERTTPAGAERLARRVLADGIAHTVLAVPIAQERATMHLDTVCTMVDA
                     DAIVMYPNVADTLSAHAVTAREPYDDESQLELAVAAAEPFLVAAAKAMGIDTLQQIDT
                     GLDPVTAEREQWDDGNNTLSIAPRVAVAYERNTETNARLRDAGIEVVAIAGSELGSGR
                     GGPRCMSCPIGRDPI"
     gene            complement(4410..5381)
                     /locus_tag="VH419_15535"
     CDS             complement(4410..5381)
                     /locus_tag="VH419_15535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020390595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5926 family protein"
                     /protein_id="HEY7045082.1"
                     /translation="MGGHLHMGKKARSKAKALPETTDGVGPRQPCPCGSGKRYKACHG
                     SARGDVYVVRTFAGLPGECDWVALREFVPVARAPLTLLPDAFDGAGKGASVSVVTVLP
                     GIAPAQVRDDGVVWVAVQVTHQSGDPSRDFAEALRLGLEGEAGTTVALPELPGAGPRL
                     QDVVDPTSTFDVEVLDGFDFWFDGSAEDEASIAATLEQLNASVEPTTRLSSVDAAYWT
                     SVGTKEHLRWVMPYDEEDVLTALARLHVADADRLGPDSRLVGSFRAHGLLVPVWDLPV
                     GTGAEAVEEPAAAFAERLGTALSATSALTDGERAARHGLANRQITIR"
     gene            complement(5485..5916)
                     /locus_tag="VH419_15540"
     CDS             complement(5485..5916)
                     /locus_tag="VH419_15540"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY7045083.1"
                     /translation="MGANAVAIPAESGDDQDQAWSVPFRAVAVRSARTTITVALRRLG
                     VSSQVIEDARIVVSELLGNALRHARPLPDGTLRVGLAVEDAAVRLYVVDGGSATLPTL
                     LNPPPMALGGRGLSIVRTLTRDWGVHETLSGNTVFGVLNLA"
     gene            complement(5975..6856)
                     /locus_tag="VH419_15545"
     CDS             complement(5975..6856)
                     /locus_tag="VH419_15545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017933432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HEY7045084.1"
                     /translation="MTKVRGLESLRGIRLRKRPPISLAQSPEPPQVVAHRGSSDAKAE
                     HTLAAYVHALDEGAEALECDVRLTADGHLVCVHDRRIDRTSNGRGVVSTLQLDQLEAL
                     DWSSWKRPWHELDDEADDPSPDSRSILTLEKLLDTIRGYDRYVELAIETKHPTRFAGL
                     VERRLVELLDSYGWARPRRGTTSRVRVMSFSWISLRRSLEMAPSMQTVYLMDRVPLRF
                     RDGSLPLGTSIAGPSIEIIRAHPEYVDKAHRAGNQVHVWTVNHPADVELCAALGVEAI
                     ITDKPGATLRQLNERTT"
     gene            complement(6925..7800)
                     /locus_tag="VH419_15550"
     CDS             complement(6925..7800)
                     /locus_tag="VH419_15550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc finger Ran-binding domain-containing
                     protein"
                     /protein_id="HEY7045085.1"
                     /translation="MQCPRCGLQNPPGITACLRCGLPAPQSQPAPVQPPQGPQPGASE
                     EQTTVIRREAPAGQGQRGGPPGQYAAQPPGQPVPAPQQPQPAGQYGGAADRPTYAGGA
                     TPSSAWPVTTQAEARAAARDAGATATLVALGIGALLALCYAVWAFTARRGVFADFADG
                     KTVSGDDAKSSDRIDTVLLVIAGVVVVIALALWLMRMVNQKTAGGTPDLVGLGVAMLG
                     VVVVLVGLFLSGQISDAGDQASQGDKGVTATLVTGAGFLLIAVGLLVGIVSVRGRPSG
                     STSYAHRAGTGYPGW"
     gene            complement(7986..8324)
                     /locus_tag="VH419_15555"
     CDS             complement(7986..8324)
                     /locus_tag="VH419_15555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011753652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEY7045086.1"
                     /translation="MEPIPEVDVTGVPKEMPPALTVLDVREDDEWAAGHIEGARHIPL
                     MQVPARFAELPSDRQVLVVCRVGSRSAQATAYLQAQGVQAVNLAGGMVAWAQAGRPMV
                     SDVADVPYVL"
BASE COUNT         1312 a         2995 c         2965 g         1379 t
ORIGIN      
        1 gcgaatgttg gttgacgggg tactaggttt gcggcatgac ggactcccct gatgtggacg
       61 acttcgaggc gacggccggt gtcgtcgacg cgtccctcga cctcggctac gcgagactcg
      121 acgtggaccg agtccgtcgg accggagacc cggaggtcgt gtacggcgcc ggcaagagcc
      181 cggaacaggt cgtagacctg ctcgaccggc tgcgcacggc gcaccccgat cgctgtgtct
      241 ttgcgacccg actcagcgac gacgcgatcg ccgccgtgcg agagcggctg cccgactcgg
      301 aggtgaacga aaccgcccgc gcggtcacct gtggcgagct gcccaggacg cgcggtctcg
      361 tcacggtcgt ctccgcgggg acatccgacg cgacggtcac ccacgaggct gccttgacgg
      421 cacgagtctt cggcgcgggg acgcgcgtca tcaacgacgt cggtgtcgcc gggctgcacc
      481 gcgtcctcgg cgtgcgcgcc gagctggagt cggcggactg cctcgtcgtc gttgccggga
      541 tggagggtgc actgccgtcg gtcgtgggcg gactgaccgg tacgccgctg gtggccgtgc
      601 ccacgtcggt cggttacggc gcctcgttcg gtggcgtggc cgccctgctc gccatgttga
      661 actcctgcgc acccggcgtc gtggtcgtca acatcgacaa cgggttcggg gccggcgtct
      721 tcgcggctcg cgtcgcgcgc aacgccgctc cgaagtcggc gaccctgacg cactgaccgg
      781 gaggacacat caggtgatcg ggtggatcga cgcatcgtcg ggagcctccg gcgacatgct
      841 tctcggcgcc ctgctcgacg ccggcgcgga cgaggacctc gtccgcgact cgatcgcttc
      901 ggtcgcaccc gagccgacga cactgacctt ggtgcgagcg cgacgcgggt cgctctgggg
      961 caaccacgcg ctcgtggaga ccgccgcctc gtcatcacat cgcggactgc gcgacgtggt
     1021 cggtctcatc agctcggccc cgttgcacgc ggacgtgaaa cgacacgcga tcgacgtgtt
     1081 caacctgctc ggcgcggccg aggccgacgt gcacggcctc accgtcgacg aggtgcactt
     1141 ccacgaggtc ggcgcggtgg acgccatcgc ggacatcgtc ggagtctgcg ccggattcgt
     1201 cggcctcggc ctctccgatc tgcactgcag cccggtcgcc gtcggatcgg gcaccgtgac
     1261 gacgagccac ggcccactga gcgtgccgcc cccggcggtg gtgcatctcc tgcacggcgc
     1321 accgacgtac gccggtccag cggtcgccga gctgtgcacg cccaccggtg ccgcgctgct
     1381 cgcgcactgg gtgacagctt ggggaccgca accgctgatg accgtggacc agatcggcat
     1441 cgggtgcggc accagggact tcgacagcca cccgaacgtc ctgcgcatct tcgtcggcgt
     1501 cgcgcatgag gcgtcggccg cacaggcggt cgtgtacgag acgaacgtcg acgacctcga
     1561 tccccgcctg tggccgacga tcctgcagcg gctcctcgac gccggcgcct ccgatgcgtg
     1621 gctcaccccg atcctgatga agaagggccg gccggcatac acgttgtcgg cgctcaccaa
     1681 cgccgccgcg gctgccgacg tacggcaggt gatcttcgcc gagacgtcgg cgatcgggct
     1741 gcgcgagacg tacgtcggca agcacgcact cgaccgcaag atggagaccg tcgaggtcga
     1801 cggacgttcg atcgcggtca agctcgcggt gagcgacggc aaggtggtca acgtgcaacc
     1861 ggagtacgac gaggtggtcg cagccgcgca ggatctggga cgtcccgtca agtctgtcct
     1921 ggccgatgcc gcagcggcgg cggcacgctt ctggcagcag tgacgacccg tcggactcgg
     1981 cggctcaggc tctgttggtt aaggtggctg atcgcgggtg tcccccgcgg cttcgccgtt
     2041 acggacatcc gatcaccgct cgacctccgt ctccgaccgt tgggctcaca tggactggca
     2101 ccttgtcttg atcagctttg cgctgatctt cccggtggag ctcccggaca agacgttcgt
     2161 cgccgccttg gtgctgtcga cgaagtaccg gccgttgttc gtctggatag gcgtcggact
     2221 ggccttcggc gtccaatgcc tggtggcggt gacggcgggc gcggcggtgt cgttcttgcc
     2281 ggagtggttc gtccacggcc tgagcacgct gatgttcctc ctcggggcga tcatcttgtt
     2341 caggagcgcc cctggtgcgg atgccgaaga gcgggagcag gaggagtcgt tcgccgagaa
     2401 ggcgacccga gagcagaagg gttggcacgc ggctgccgcc tccttcgccg tcttgttcgc
     2461 ggcagagtgg ggtgacctct cgcagatcct gaccctgaac ctggtggcca agtacggttc
     2521 cccgctcgag gtcttcatcg gttcgtgggc ggcgctgctg gccgtctcag gactggccgt
     2581 gatcggtggc cgggtgctgc tgcgctacat gcggttgtcg ttgttgcact acttcgcggg
     2641 cagcgtctgc ctgatcttcg tcgtggtcgg gatcgtgcag ctcatcaacg actgacaccg
     2701 caagcaggac acctgagcag cgcaaagcgg cgtctgttcg cggacgccga ttaggctcag
     2761 cctcatgacg gatgcgcctg ctccattggg cgccgacagc gaggtcggcc ggctgcgtac
     2821 ggttctgctg caccgccccg gcccggagct caagcgactc accccacgca acaacgacag
     2881 cctgctgttc gacggcatcc cgtgggtcgg aagggcgcaa gacgagcacg acgcgttcgc
     2941 cgcggcgctc cgggaccgcg gagtcgaggt gctgtacctc gtggagctgc tggtcgagac
     3001 gctggccgac cccgatgcgc gagcgcaggc gatcggccac gtgacggcga gccttcggct
     3061 cggtgacacg ttgcgcgcgc atctcggccg ttggctgcac agcctgtcgg ccgacgagct
     3121 ggcggcggcc atcaccgccg gcgtacgcaa cgacgaggta cgcggcggct tcggtctggt
     3181 gacagcgttg ctcgcgcacg acgacttctt gatcgacccg ctgcccaacc tgttgttcac
     3241 acgtgactcg tcgttctggc ttcgcgaccg ggtcgcggtg gcgagcctgg ccatgcccgc
     3301 ccgcacgcgg gagacacagc tgatggagct ggtctacacg ttccaccctc gcttctccgg
     3361 ggtggaacgc atccacggct ggcgggccga gcatgtcgaa ggcggcgacg tcctgctgct
     3421 cgggccgggt gtcatcgccg tcggcgtggg cgagcgtacg acgcccgccg gtgccgagcg
     3481 gctggctcgt cgcgtgctgg ccgacgggat cgcccacacc gtgctcgcgg tgccgatcgc
     3541 gcaggagcga gccaccatgc acctggacac ggtctgcacg atggtcgacg cggacgcgat
     3601 cgtcatgtac ccgaacgtcg ccgacacgct gtctgcccac gcggtgacgg ccagagaacc
     3661 gtacgacgac gagtcgcagc tcgagctcgc ggtggccgcc gccgagccgt tcctcgttgc
     3721 tgccgccaag gcgatgggga tcgacacgct gcaacagatc gacaccggtc tcgacccggt
     3781 gaccgctgaa cgcgagcagt gggacgacgg caacaacacg ctgtcgatcg cacccagggt
     3841 ggctgtggcc tacgaacgca acaccgagac caacgcgcgg ttgcgggacg cgggaatcga
     3901 ggtggtggcg atcgctgggt cagagctggg ctcgggccgg ggtggtccgc ggtgcatgtc
     3961 ctgcccgatc ggccgggacc cgatctgacc aacccggacg acgcagtcgc gatggccgaa
     4021 tccgttggcg actcgggtgc ggcgacggtc ttcatcccgg ccgttcggac gctcccggga
     4081 caacgcgttc cgcgccgtgg aacgaccgat tctgactcac gagtcagttt tcttcctgac
     4141 tcgcgagtca ggagcggagc cgaaaacccc tatcttgcag gggaaatgga gaacccggcc
     4201 gttactgacg ggggatacag caacgaccgg gtgtgcttga agcataaagc gactgcgggc
     4261 gtgacacaag ccgaagccac gcatccgtga cgggaatctc aaagagttgc cgcgtggtca
     4321 cagagagcgc cgacaccgtc ggcttctgca ctgagccgac gctcgcgcag cacatcatga
     4381 ggccgtgtgt ctgcacggct ggcggcgctt cagcgaatgg tgatctgccg attcgcgaga
     4441 ccgtgcctgg ccgcccgttc gccatcggtc aacgccgagg tcgcggacaa cgcagtgccc
     4501 agccgttccg cgaacgcggc cgccggttcc tccaccgcct ccgcgccggt cccgaccgga
     4561 agatcccaga ccggcaccag cagaccgtga gcacggaagg agccgacgag ccgtgagtcc
     4621 ggcccgagcc ggtccgcgtc cgcaacgtgc aggcgtgcaa gggcggtgag gacgtcctcc
     4681 tcgtcgtacg gcatgaccca acgcagatgc tccttcgtac cgaccgacgt ccagtacgcc
     4741 gcgtccaccg acgacagtcg agttgtcggt tcgaccgagg cgttgagctg ttcgagggtg
     4801 gctgcgatcg acgcctcgtc ctccgccgac ccgtcgaacc agaagtcgaa gccgtcgagc
     4861 acctcgacgt cgaacgtgct cgtcgggtcg acgacgtcct ggagccgcgg tccggcgccc
     4921 ggcagctccg gcagcgcgac cgtcgtgccg gcctcgccct cgagcccgag ccgcaacgcc
     4981 tccgcgaagt cacgactcgg gtcgccggac tggtgcgtga cctggaccgc cacccacacc
     5041 acaccgtcgt cacgaacctg cgcgggcgcg atccccggga gcacggtcac cacagacacc
     5101 gatgcaccct tgccggcgcc gtcgaacgcg tcgggcagca gcgtcagcgg cgcccgcgcc
     5161 accgggacga actcacgcag ggccacccag tcgcactcgc ccgggagtcc cgcgaacgta
     5221 cggacgacat acacgtcacc gcgcgcgctc ccgtggcacg ccttgtagcg cttgcccgac
     5281 ccgcacggac acggctgccg cggacctacc ccatcggtcg tctccggcaa cgccttcgcc
     5341 ttgctccgag ccttcttgcc catgtggagg tgccctccca tccgtcaatc ttgacgcttg
     5401 ccgcaggcca tcattgccga ggcgaacggc caccaggtgg cttggtgtgc cggacccggc
     5461 gactccgtcg ctcgcgtcgg tccatcaggc caggttgagg acgccgaaca cggtgttgcc
     5521 tgacagggtc tcgtgcacac cccagtcgcg ggtcagcgtt cgtacgatcg acaggccgcg
     5581 cccaccgagc gccatcggcg gcgggttcag caacgtcggc aacgtggccg atccgccgtc
     5641 gaccacgtac agccgcacag cggcgtcctc gacggcgagg ccgacgcgca aggtgccgtc
     5701 cggcagcggc cgggcgtgac gcaatgcgtt gccgagcagc tcactgacca cgatccgggc
     5761 atcttcgatg acctggctgg agacgccgag ccgccgcagc gcgaccgtga tcgtcgtgcg
     5821 cgccgagcgc acggcgaccg cccggaacgg gacgctccag gcctggtcct ggtcgtcacc
     5881 gctctcggct gggatcgcca cagcattcgc ccccacctcc aacactgtga ccagtgggac
     5941 tcgagttcgc aacctacccg gcagatcccg agggtcaggt cgttcgctcg ttcagctgtc
     6001 gcaaggtggc acccggcttg tcggtgatga tggcttccac accgagcgcc gcgcacagtt
     6061 ccacgtccgc cgggtggttg accgtccaca catgaacctg gttgccggcg cgatgggcct
     6121 tgtcgacgta ctccggatgg gcccggatga tctcgatcga cggaccggcg atgctggtgc
     6181 cgaggggcag cgagccgtcg cggaatcgca acggcacacg gtccatcaga tacaccgtct
     6241 gcatcgaggg tgccatttcg agggagcgtc gcaggctgat ccaggagaag ctcatgaccc
     6301 ggacccgcga cgtcgtgccg cgccgtggcc gagcccagcc gtagctgtcg aggagctcga
     6361 cgagccgtcg ctccaccagc ccggcgaacc gtgtcggatg cttggtctcg atcgccagtt
     6421 cgacgtaccg gtcgtagccg cggatcgtgt ccagcagctt ctccagggtg aggatggacc
     6481 ggctgtccgg cgaagggtcg tctgcttcgt cgtccagctc gtgccacggt cgcttccacg
     6541 acgaccagtc caaggcctcg agctggtcga gctggagcgt ggacaccacg ccgcggccgt
     6601 tgctggtgcg gtcgattcgg cgatcatgca cgcagaccag atgaccgtcc gcggtgagcc
     6661 ggacgtcgca ctcgagagct tcggctccct cgtcgagcgc atggacgtac gccgccagcg
     6721 tgtgttcggc cttcgcgtcg ctggagccgc gatgggcgac gacctgtggg ggttcgggtg
     6781 actgcgccaa ggagatcgga ggtcgcttgc gtagacgtat cccgcggagg ctttccaagc
     6841 ctcgcacctt cgtcacgtct caatcgtgac acgccaggag cggccatcgg gcccgcgtcc
     6901 ggtttgtgct cccgcgagtt gtggtcacca cccggggtag ccggtgccgg cacgatgggc
     6961 gtagctcgtc gatccgctcg gtcgacctcg gacggagacg atcccgacca gcagcccgac
     7021 cgcgatcagg aggaatcccg cgccggtgac gagtgtggcg gtgacgccct tgtcaccctg
     7081 gctcgcctgg tcgccggcgt cggagatctg cccggagagg aacaacccga cgagcaccac
     7141 gacgacaccg agcatcgcaa cacccaggcc gaccaggtcg ggagttccgc ctgctgtctt
     7201 ctggttgacc atccgcatca gccacagcgc gagcgcgatg acgacgacga ctccggcgat
     7261 gacgaggagg acggtgtcga tccggtcgct ggacttggcg tcatcgccgg acaccgtctt
     7321 accgtcggcg aagtcggcga agacgccccg gcgggccgtg aacgcccaca ccgcgtagca
     7381 cagggccagc agcgcgccga tgcccagtgc gacgagcgtc gcggtcgcgc cggcgtcgcg
     7441 agcggcggct cgcgcctctg cctgggtcgt caccggccag gccgagctcg gcgtggcacc
     7501 gccggcgtac gtggggcggt ccgcagctcc gccgtactgc cctgctggtt gcggctgctg
     7561 cggagcggga acgggctggc ccggtggctg cgcggcgtac tggcccggcg gtccgcctcg
     7621 ttgaccctgg cccgcgggcg cttcccggcg gatgaccgtg gtctgctcct cagacgcgcc
     7681 gggctgtggt ccctgcggcg gctgcaccgg cgccggctgg ctctgcggtg ccggcagacc
     7741 gcagcggagg caggccgtga tcccgggcgg gttctgcaga ccacagcgag gacactgcat
     7801 cacgggctct ccccttgtga gcgctggcga gcacagtcag ccgacgtgtg cggatacgac
     7861 ctgccgagcg tacccaagca gcagcccacc acggcccgag aatccgaacg ccgggctacg
     7921 ggccctggcc cacgacgcac cgtgacatgg gcaccgatcg atgcctcgag agtcggtcag
     7981 ccggtctaga gcacgtatgg cacgtcggcg acgtccgaga ccatcggacg gcctgcctgt
     8041 gcccacgcga ccatgccacc cgccaggttg accgcctgca caccctgtgc ctgcaggtac
     8101 gccgtcgcct gcgccgaccg gctgcccacc ctgcacacaa ccagcacctg acggtcggag
     8161 ggaagctccg cgaagcgtgc cgggacctgc atgagcggga tgtgccgagc accttcgatg
     8221 tgaccggccg cccactcgtc gtcctcgcgc acgtcgagga cggtgagcgc cggtggcatc
     8281 tccttcggaa ccccggtcac gtccacctca ggtatcggct ccatcacggc tccagcctgc
     8341 cacgggacca cgcgacgtcc ggaccggcac tgcccgggcc tcaggttctg gtgggcgcag
     8401 tggtcgcgat cggcggacgt gttccccggg cgctccgtcg tcatgggctg aggtggattg
     8461 gcagtgagga gcgggagagc tatgaggtcc tgcggtccgg cggctgatcc ggccggcttt
     8521 ccgtcccgtt ggtgggtggg tgtgactggc ggttttcgca catccgccgg tcagcgagct
     8581 cggtttgcat ggctaaccag ccaaacccct ccctgcccag gcagacctgc ctggctaccc
     8641 gtccgtttgg a
//
LOCUS       DATBFZ010000505         3992 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_4490218, whole genome shotgun sequence.
ACCESSION   DATBFZ010000505 DATBFZ010000000
VERSION     DATBFZ010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 3992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3992
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4490218"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(138..1679)
                     /locus_tag="VH419_15560"
     CDS             complement(138..1679)
                     /locus_tag="VH419_15560"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY7045087.1"
                     /translation="MPETIPGLLAAAAERDPSGVWVHTDDGTQTYAEAAAQTARLAER
                     LQAEGVRKGDLVVVTTRTTPPYLLCWLALASLGAITVPTDPRGTAEELAGLMRQVRPR
                     MIVTDAGLTPLVDEAGVAGLAELGVLDIDDAVGGWPADTAGGPRLRVDVEPGDLAVLI
                     PTSGTTGRSKLVMQTHRAYAMAGEGFPYWMELDSSDRLMTSLPLFHINAPAYSVMGSL
                     AAGAGLVLVPRFSASGFLDTARRHRATEFNAIGAMLEVLIRQPERADDADTDLRVCYT
                     GPSPAKEWQEAFERRFALRVVCGYGLSESPYGLIWRHGTRPFGTLGWVRQHPTLGTVN
                     EARVIDDDGTDVTTGDTGELLLRNPTITPGYWEMPDETAKTIVDGWLHTGDLVTANAD
                     GTYTFVSRRKEVLRRRGQNLSPAEVEDVIATHADVLEVAVVAVPSELTEDEVKAFVVP
                     KPGRDLDFAALRAWTAEHLSAFKVPRFWQSIDALPRTPTARVAKHKLPSGHPSEEYDA
                     DQQGQ"
     gene            complement(1694..2752)
                     /locus_tag="VH419_15565"
     CDS             complement(1694..2752)
                     /locus_tag="VH419_15565"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HEY7045088.1"
                     /translation="MDADQVAHALPSLFPTSLVGSYAQPDWLIDRERLAGRFPPRVRA
                     RELWRPAPEHLREAQDDATLLAIREQEQAGLDIITDGEIRRESYSNHFATALGGVDID
                     NPGEALDRSGHPNPVPRVVGPIERPEPVQVRDTQFLRAHTSRTIKVTVPGPFTMSQQA
                     QNDYYADAESLAMAYADACNAEVKDLFAAGADIVQLDEPYLQARPDPARAYGVKALNR
                     ALDGVVGTTAVHLCFGYAAIIHDRPSGYSFLSELADCSCDQVSIETAQSGLDPGVLGT
                     LDGKTVILGVLDLSDHEVETPEVVAQRVHRAMAVVDPARIVLAPDCGMKYLPRASAEG
                     KLRSMAAAAQLLRAELAG"
     gene            2953..3948
                     /locus_tag="VH419_15570"
     CDS             2953..3948
                     /locus_tag="VH419_15570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7045089.1"
                     /translation="MNEPKEPAGTAEPDGVEPENGLDSTPDSSPRTTLVERPRWLVVV
                     VVTLLGTCGLVAAWLVNPGDPQGFWQNVLIELSGAVALLGVLILLERAMMRHVQAVVR
                     ELEERLDERREEGPDRLQQDVRSVVQRSEATAEGVRRLGLAEEARAVADEAMRRELLR
                     IASTLEENEVNLRCTLRAEVPPNSNVLGWHPNNFRRGDVSKQAPRCGEGRGSDRGWQL
                     DVRAAIGARVKWTLAAATRLCPDGQCWLAVGSIVEPINPLGEGADRFSLSWENDSLKR
                     LIGFRRVHAPDPSLSDLPQVIDAAKKDLAGWVAESLDVFYELTEWSAESEHYSRR"
BASE COUNT          602 a         1297 c         1449 g          644 t
ORIGIN      
        1 cttgatctcg acgggctcga tcagcgtggg tacggcggtc gagcttgtcg agaccctgga
       61 ggatcggtgc gcgtgtgatc tcgacgggct cgatcaccgg gggtacggcg ggtggagcgg
      121 gtcgagacct cggacgatca ctggccctgt tggtcggcgt cgtactcctc tgaggggtgg
      181 ccggacggca gcttgtgctt ggccacgcgc gcggtgggcg tgcgaggcag tgcgtcgatc
      241 gactgccaga agcgcggaac cttgaacgcc gacagatgct cggcagtcca cgcgcggagt
      301 gcggcgaagt cgaggtcgcg cccgggcttg ggtacgacga atgccttcac ctcgtcctcg
      361 gtcagctccg atgggacagc caccacggcc acttcgagga cgtccgcgtg cgtcgcgatg
      421 acgtcctcca cctccgccgg cgacaggttc tggccacggc gacgcaggac ctccttgcgg
      481 cgggacacga acgtgtaggt tccgtcggcg ttggccgtga cgaggtctcc cgtgtgcaac
      541 cagccgtcga cgatcgtctt cgcggtctcg tccggcatct cccagtaccc cggcgtgatg
      601 gtcgggttgc ggagcagcag ctcgccggtg tcgccggtgg tcacgtcggt gccgtcgtcg
      661 tcgatcacgc gcgcctcgtt cacggtgccg agggtcggat gctgccgcac ccagccgagc
      721 gtgccgaacg gtctggtgcc gtgccgccag atcaggccgt acggcgactc ggacagcccg
      781 tagccgcaga cgacgcgaag cgcgaaccgg cgctcgaagg cctcctgcca ctctttggcc
      841 ggtgacggcc cggtgtaaca cacgcgcagg tcggtatcgg cgtcgtcagc ccgttccggt
      901 tgtcgtatga ggacctcgag catcgccccg atcgcgttga actcggtcgc tctgtgccgg
      961 cgagctgtgt cgaggaaccc gcttgcagag aagcgcggga cgagcacgag gccggcgcct
     1021 gctgccagcg aacccatcac ggagtacgcc ggcgcgttga tgtggaagag cggcagcgac
     1081 gtcatcaggc ggtcggagct gtcgagctcc atccagtagg ggaagccctc gcccgccatg
     1141 gcgtacgcgc ggtgcgtctg catgacgagc ttggaccggc ctgtggtgcc ggacgtcgga
     1201 atgaggaccg ccaagtcgcc gggctcgacg tcgacccgaa ggcgtgggcc gccggcggtg
     1261 tcggcaggcc agccaccgac ggcatcgtcg atgtccagta cgccgagctc ggcaaggccc
     1321 gccacccccg cctcgtcgac gagcggcgtc agaccggcgt cggtgacgat catccgagga
     1381 cgtacctggc gcatgaggcc tgccaactct tcggcggtcc cgcgtgggtc ggttggcacg
     1441 gtgatcgcgc cgagggacgc cagcgccagc cagcagagca gatacggcgg cgtcgtccgg
     1501 gtcgtgacga cgaccagatc ccccttacgg acgccttcgg cctgcagccg ctcggcgagt
     1561 ctcgccgtct gagccgcagc ctcggcgtac gtctgggtgc catcgtctgt gtgcacccac
     1621 acgccggacg ggtcacgctc ggccgcagcc gccagcaggc cggggatcgt ctcgggcatc
     1681 gtccgcgtgc cgttcagccg gcgagctcgg cgcgcaacag ctgtgcggcc gcggccatcg
     1741 agcgcagctt gccctcggca cttgcgcgcg gcaggtactt catcccacag tccggcgcca
     1801 gcacgatgcg tgcgggatcg accacggcca tcgcccggtg aacccgctgc gccaccacct
     1861 caggtgtctc cacctcgtgg tccgagaggt cgagtacgcc gaggatcacg gtcttgccgt
     1921 cgagcgtccc gagcacgccg gggtcgagcc cggactgcgc ggtctcgatc gacacctggt
     1981 cgcacgagca gtccgccagc tcggagagga acgagtagcc agacgggcgg tcgtggatga
     2041 tcgcggcgta cccgaagcac aggtgcacgg cggtcgtccc gacgactccg tcgagcgccc
     2101 ggttgagcgc cttgacgcca tacgcgcgcg ccgggtcggg ccgtgcctgg aggtagggct
     2161 cgtcgagctg gacgatgtcg gctccggcgg cgaacaggtc cttgacctcg gcgttgcagg
     2221 cgtcggcgta cgccatggcc agcgactcgg cgtcggcgta gtagtcgttc tgcgcctgct
     2281 gcgacatcgt gaacggtccg gggacggtga ccttgatcgt gcggctggtg tgcgcccgca
     2341 ggaactgcgt gtcacgcacc tggaccggtt ccgggcgctc gatcggaccg acgacacgcg
     2401 gcaccgggtt ggggtggcca ctgcggtcga gcgcctcgcc gggattgtcg atgtcgacgc
     2461 cgccgagcgc ggtggcgaag tggttggagt agctctcccg acggatctca ccgtcggtga
     2521 tgatgtcgag tccggcttgc tcctgctccc ggatcgcgag gagggtcgcg tcgtcctgag
     2581 cttcgcggag gtgttccggt gcgggccgcc acagctcgcg ggcgcggacg cggggcggga
     2641 accggccggc cagtcgctcc cggtcgatga gccagtccgg ctgcgcatag ctgccgacca
     2701 gtgaggtcgg gaacaacgat ggcaaggcgt gcgcgacctg gtcggcgtcc atccgctcag
     2761 tcttgctgag ccgtcaacca ccggcaacgg tcgccaggtc cggggggtcc gcgcatgggc
     2821 agcccgtccc tctgagctca ggttgagtgc agcgtgggcc ggccactggg tctgctctct
     2881 ggtcccgcgt cggactgaga acgcttgccc gcacgagcaa ccgggagtcc aatgactcaa
     2941 cgagggggcg ggatgaacga accgaaggag ccggcaggca cggccgagcc ggacggcgtc
     3001 gagccggaga acggcctcga cagcactccg gacagctcgc cacgaacgac gttggtggaa
     3061 cggccgcgat ggcttgtcgt cgtggtcgtg acgctgttgg gcacctgcgg tctcgtcgca
     3121 gcctggctgg tgaaccccgg agacccgcag ggcttctggc agaacgtctt gatcgagctg
     3181 agcggcgccg tcgcgttgct cggcgtactg atcctcttgg aacgggccat gatgcgacat
     3241 gtccaggcag tggttcgaga gctggaggag cgcctggacg aacggcggga ggagggtccg
     3301 gaccgactgc agcaggacgt acgcagcgtc gtgcaacgtt ctgaagccac cgccgagggg
     3361 gtacggcgac tgggccttgc cgaagaggcg cgggcggtcg cggacgaggc aatgaggagg
     3421 gagctgcttc gcatcgcttc gacgctcgag gagaacgagg tcaacctgcg ctgcacactt
     3481 cgcgccgaag tccctccgaa cagcaacgtc ctcgggtggc atccgaacaa ctttcgccgt
     3541 ggtgacgtct cgaagcaggc accgcggtgc ggcgaaggcc gcgggtctga ccgcggctgg
     3601 cagctcgacg ttcgcgcggc gataggcgcc cgcgtcaagt ggacgttggc agccgcgacc
     3661 cggctgtgtc ctgacggcca gtgctggctt gctgtcggat ccatcgtgga gccgatcaac
     3721 ccgctcggag aaggcgccga ccggttcagc ctctcgtggg agaacgactc cctcaaacgg
     3781 ctcatcggat ttcgtcgcgt ccacgcccct gatccgagcc tgtcagatct cccgcaggtc
     3841 atcgacgcgg cgaagaagga cttggccggt tgggtggcgg agtcgctgga cgtgttctac
     3901 gagctgactg aatggtccgc ggagtccgag cactacagcc gtcggtgagg tgcacttttg
     3961 cggtctgatg actcggccga ggcggctggt ga
//
LOCUS       DATBFZ010000506         8917 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_5474337, whole genome shotgun sequence.
ACCESSION   DATBFZ010000506 DATBFZ010000000
VERSION     DATBFZ010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 8917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8917
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5474337"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1442
                     /locus_tag="VH419_15575"
     CDS             <1..1442
                     /locus_tag="VH419_15575"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HEY7045090.1"
                     /translation="DGMGGAPAGDHASSLAVSTMRRLDSAPPDDLPEALAGAVHRAND
                     RLSEAIEEDRAVEGMGTTIDAVLFDGARIGVAHLGDSRGYLWRDGELLRLTSDHTWVQ
                     SLIDEGRITEEEAKVHAHRSLLLKVLDGRHDNDPDLTLYDVQAGDRILLCSDGLSSFV
                     PHDRLERVLSIGTADSVAHELVTLALEAMSNDNITVVIADVVDDGPDDAAEPIVVGAA
                     AEPHRTGRSRLRAWAHREEPEEAMLLPDDIDPEEIRYAPREPRRYRWLFRAIVAIVAL
                     AVVVGLGMVAYGWSQDQYYVAAADGKVAIYQGVQADLPGVDLHHVYETQDLTLDELPS
                     YRRSQVIEGLGADDLDDARQIVGRLESFAQTCAQEATSTSEQTTTTETTRATNTTRPG
                     SSPRTTPAHRPNTGKTPGGGAVTTKPTRAAGGGPGAGHATRTTRHTEPATTGSTRTGG
                     SGAHSQPPEECAGATPSAGSAAVTTGTTR"
     gene            1439..2815
                     /locus_tag="VH419_15580"
     CDS             1439..2815
                     /locus_tag="VH419_15580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013866110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEY7045091.1"
                     /translation="MNSATFTSAPGRIVSSFVPRKRRGAELFLLILALIVGVSSYALV
                     GLGRDGEVPTDIVEYGGFLVLAGVVCHIAVRMLAPYADPVLLPAVVALNGIGLAMIRR
                     IDLGSNTHFGRQQLLWAGISVVGFVATLLVVRDHRRLQSFTYTMGFVGIALLLLPLFP
                     LIGANIHGARIWIHVGSLSFQPGEVAKIALAIFFSGYLVVKRDALALAGRRFLGVDLP
                     RGRDLGPILLMWLISLGVLVFQRDLGSSLLFFGLFVVLLYVATERPSWLAVGALLFVA
                     GAYLGYLAFGHVQSRIDDWLDPERTGQLMQGQYGLAWGGILGRGWGQGSPFLTPFSYS
                     DFIATSLGEELGLTGLMAIIVIYGLIVERGFRTALVCRDPFGKLLAVGLSMAIALQVF
                     VVVGGVTRLIPLTGLTTPFLSYGGSSLVANWIIVALMLRISDHARRPPPDLRMPSADD
                     ETQVVKLR"
     gene            2812..4278
                     /locus_tag="VH419_15585"
     CDS             2812..4278
                     /locus_tag="VH419_15585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020575025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEY7045092.1"
                     /translation="MNKPIRTLAVGCLLLFGLLLLNVNYVQVFRATSLNDAGANTRAR
                     DAECSRERGPILVDGETVARSVPSNDSLKFQRRYIDTPMYANLTGFFSCIYGTTAIEN
                     TENSILSGSDERLFVNRMVDLVGNEQPKGGSVLLTIDPKAQRAALDGLQDLTRQFGPT
                     KGAVVALDPTTGAILAMVTQPSYDPNLIASHDPTKARRAFNAVVNAPDQRGLNRGAQS
                     IYPPGSTFKLVTSAAALSNGYTPDSTVKGGTVFDLPQSSDTIHNENGSDCGGSQITLR
                     HALEVSCNVAFADLGVKLGAQTLQEQAEKFGFNDDVLDELPAATSVFPTDLDEPQTAL
                     SAIGQYDVAASPLQMALVGAGIANGGAVMKPYIVQQVRSPDLDVLREADPQVLHQAVS
                     ASVASELTQMMVDVVQSGTGTNTQIPGVTVAGKTGTANSAEDRAPYAWMVSFAPADDP
                     QVAVAVIVEQTGVSRSEISGNGLAGPIARSVMEAVINR"
     gene            4275..4868
                     /locus_tag="VH419_15590"
     CDS             4275..4868
                     /locus_tag="VH419_15590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7045093.1"
                     /translation="MMTAVGNGRSRPPASKPTPPRRTPYALRALQGSSSPATSPAARH
                     RRQAWLSIAAAAGAVLLGLGLAYAFTHGGGPDQGAVGRTAGQTFTGPVDSGKGERTAP
                     PTAPAAPVTLTVDPSDYVGRDAKDVEVELERLGLAVHVEHIEAGEKGTVAALAPTGAL
                     EEGQQVTLSVYKGTSGQPDHSGDFGPASDKQRDHEED"
     gene            4878..6638
                     /gene="pknB"
                     /locus_tag="VH419_15595"
     CDS             4878..6638
                     /gene="pknB"
                     /locus_tag="VH419_15595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012917746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEY7045094.1"
                     /translation="MDKTMIGHGRYQMGALLGRGGMAEVHRAEDLRLGRPVAIKRLRV
                     DLASDPTFQARFRREAQSAASLNHPTIVAVYDTGEEEAPDGSGLSLPYIVMELVEGRT
                     LRDILREGRRILPERALEITSSVLEALDYSHRAGIIHRDIKPANVMLTPSGDVKVMDF
                     GIARAIADSSATMTQTAAVVGTAQYLSPEQARGEQVDARSDLYSAGCLLFELLTGRPP
                     FVGDSPVSVAYQHVREDAVPPSAFDPELSPAIDAITLKALAKSVDQRYQSASEMRADI
                     MRALEGRSVLAPAVAMEETQQFLPPPPTDSTPTAVTPAMATEDREEGRRRVGLWILLA
                     ALVVAALAAGLYYAFATTGDGVTTTTVPNVQGLTLDQAEKKIEDANLVVGDETPENSD
                     TVKKGLVLRQSETPNATVDEGTVVDLTYSAGQKTAIVPDVTDMNLTDATAALEDRGFN
                     VIPKQDTESPEAHNQVTRTDPGPSTRLPVGSNVTVFYSTGFREVPNVVGQTERNATHL
                     LQSLGFVVVPSDIQSDQPEGQVVAQNPPGESRQPFGSTVVIQVSLGPTETTPIETTPT
                     ETTPTETTPTETTTETTPTP"
     gene            complement(6738..7388)
                     /locus_tag="VH419_15600"
     CDS             complement(6738..7388)
                     /locus_tag="VH419_15600"
                     /inference="COORDINATES: protein motif:HMM:NF017734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HEY7045095.1"
                     /translation="MINARGTDLRGGRNNDLTGVVADQSQRVKDLRVQAQTVQRSIDE
                     LTAAINDPQVNQLNRKLERLAAPAGMQRLAGPGLVVTLDDAPLDQDVPDGTNPNLLVV
                     HQQDLQAVINALWAGGAEGISLQGQRIIGTTGIKCVGNTVRLQGVPYAPPYRIVAVGD
                     QVAMYDALTNSPDVQDYRAFTEPPYNLGWDLRTETRVRVPAYKQPITLEYAQPLTD"
     gene            7663..7914
                     /locus_tag="VH419_15605"
     CDS             7663..7914
                     /locus_tag="VH419_15605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020575029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CrgA"
                     /protein_id="HEY7045096.1"
                     /translation="MPESRSRKKADYTPPTSSKKPARVGSRRWVAPVMVGCWLFGLAW
                     IVVFYLAPDTKYLRDLGNWNLAVGMFFIALGFVFSTKWE"
     gene            complement(8126..>8917)
                     /locus_tag="VH419_15610"
     CDS             complement(8126..>8917)
                     /locus_tag="VH419_15610"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEY7045097.1"
                     /translation="ICPDCMRQASVGFQCPDCVKRGATQSRSVRTVYGGRVASGSNVV
                     TISLIAINVLVWIVAVATGGVDGKFARTLILLPQGGPTRLSNGQVVIVDGVADGSYWQ
                     LVTSTFLHTQPLHILLNMVGLWIFGSFLEVALGRWRYLLLYLLSGLAGSVAIYWLAPP
                     GSFSLGASGAVFGLFGASLVILLRQHRDVTQLLILLGLNLVFSFTASNVSWQAHVGGL
                     VAGLVMGAGFAYTPRSQRTPIHAGMLIGVGVVLVLAALARTAALT"
BASE COUNT         1482 a         3120 c         2996 g         1319 t
ORIGIN      
        1 ccgacgggat gggtggggcg ccggccggcg accacgcgtc ctccctggcc gtcagcacga
       61 tgcgtcggct cgactccgcg ccgcccgacg acctgcccga ggccctcgcc ggggccgtgc
      121 accgcgccaa cgaccggctc tcggaggcca tcgaggagga ccgtgcggtc gaaggtatgg
      181 ggacgacgat cgacgccgtc ctgttcgacg gtgcccggat cggcgttgcc catctcggtg
      241 actcgcgcgg ctacctgtgg cgcgacggag agctgctccg cctcaccagc gaccacacct
      301 gggtgcagag cctcatcgac gagggccgga tcaccgagga ggaggcgaag gtgcacgcgc
      361 accgctcgct cctgctcaag gtcctcgacg gccggcacga caacgacccc gacctgacgc
      421 tgtacgacgt gcaggcggga gatcgcatcc tgctctgcag cgacgggctc tccagcttcg
      481 tcccgcacga ccggctcgag cgtgtcttgt ccatcggcac ggccgacagc gtggcccacg
      541 agctcgtgac gctcgcactg gaagcgatga gcaacgacaa catcaccgtc gtgatcgccg
      601 acgtcgtcga tgacggcccc gatgacgcgg ccgagccgat cgtcgtcgga gcagccgccg
      661 agccgcatcg caccggacgg agccggctgc gtgcctgggc acaccgcgag gagcccgagg
      721 aggcgatgct gctcccggac gacatcgacc ccgaggagat ccgctacgcg ccgcgtgagc
      781 cgaggcgcta ccggtggttg ttccgcgcga tcgtcgccat cgtcgcgctc gcggtcgtcg
      841 tcgggctggg catggtcgcc tacggctgga gccaggacca gtactacgtc gcggccgccg
      901 acgggaaggt cgccatctac cagggggtgc aggccgacct gcccggggtg gacctgcatc
      961 acgtgtacga gacgcaggac ctcaccctgg acgagctgcc gagctaccga cgcagccagg
     1021 tgatcgaggg gctcggggcc gacgacctcg acgatgcacg ccagatcgtc ggtcggctcg
     1081 agtcgttcgc gcagacctgc gcccaagagg ccacttcgac gagtgagcag acgaccacga
     1141 cggagacgac cagagcgacg aacaccacca ggcccggatc atcgccacgc accaccccgg
     1201 cacaccgtcc gaacaccggc aagacccccg gcggcggggc tgtcacgacg aagccgaccc
     1261 gagccgccgg aggtggtccg ggcgcaggac acgccaccag gacgacccga cacaccgagc
     1321 cggccacgac cggcagcacg cgcaccggcg gatcgggagc gcacagccag ccaccggagg
     1381 agtgcgcggg cgccaccccc agcgccggct cggccgccgt cacgacgggg acgacccgat
     1441 gaacagcgcc acgttcacca gcgcgccagg ccggatcgtc agctcgttcg tgccgcgcaa
     1501 gcgccggggc gccgagctct tcctcctcat cctcgcgctg atcgtggggg tcagctccta
     1561 cgcgctcgtc gggctcggca gggacggcga agtgccgacc gacatcgtgg agtacggcgg
     1621 cttcctcgtc ctcgccggcg ttgtctgcca catcgccgtc cggatgctcg cgccgtacgc
     1681 cgacccggtc ctgctgcccg cggtggtcgc cttgaacggc atcgggctgg cgatgatccg
     1741 ccggatcgac ctgggcagca acacccactt cgggcgtcag cagctgctct gggcaggcat
     1801 ctcggtggtc gggttcgtcg ccaccctgct cgtggtccgc gaccaccgca ggctccagtc
     1861 gttcacgtac accatgggct tcgtcggcat cgccttgctc ctgctgccgc tctttccgct
     1921 gatcggcgcg aacatccatg gcgcacggat ctggattcac gtcggcagcc tgtcgttcca
     1981 gcccggcgag gtcgccaaga tcgcgctggc gatcttcttc tccggctacc tcgtggtcaa
     2041 gcgcgacgcc ctggccctgg ccggccgccg gttcctcggt gtcgacctgc cccgtggacg
     2101 cgacctcggt ccgatcctgc tgatgtggct gatcagcctg ggcgtgttgg tgttccagcg
     2161 cgacctgggg tcctcactgc tgttcttcgg cctcttcgtc gtactgctct acgtcgcgac
     2221 cgagcggccg agctggctgg cggtcggggc gttgctgttc gtcgccggcg cgtacctcgg
     2281 gtacctcgcg ttcgggcacg tccagtcccg gatcgacgac tggctcgacc ccgaacgcac
     2341 cgggcagctc atgcagggcc agtacgggct cgcatggggc ggcatcctgg gccggggctg
     2401 gggacagggg agcccgttcc tgaccccctt ctcgtactcc gacttcatcg cgacctcgct
     2461 cggtgaggag ctcggtctga ccggcctgat ggcgatcatc gtcatctacg ggctcatcgt
     2521 cgagcgcggc ttccggacgg cgctggtctg ccgtgacccg ttcggcaaac tgctcgcagt
     2581 gggcctgtcg atggcgatcg ccctgcaggt gttcgtcgtc gtgggtggcg tgacgaggct
     2641 catcccgctg accggcctca ccacgccgtt cctgtcgtac ggtggctcgt ccctcgtggc
     2701 gaactggatc atcgtggcgc tcatgctccg gatcagtgac cacgcccggc ggccaccgcc
     2761 tgacctgcgg atgccgtccg ccgacgacga gacccaggtg gtgaagctgc gatgaacaag
     2821 cccatccgca cgctcgccgt cggttgcttg ctgcttttcg ggctgctgct gctgaacgtc
     2881 aactacgtcc aggtgttcag ggccacctcg ctcaacgacg ccggtgccaa cacccgtgcc
     2941 cgggacgccg agtgctcccg cgaacgcggc ccgatcctcg tggacggcga gacggtggcg
     3001 cgcagcgtcc cgagcaacga cagcctgaag ttccagcgtc gctacatcga cacacccatg
     3061 tacgcgaacc tcaccggttt cttctcctgc atctacggaa cgacggccat cgagaacacc
     3121 gagaactcga tcctctccgg cagcgacgag cggctgttcg tcaaccgcat ggtcgacctg
     3181 gtcggcaacg agcagccgaa gggcggcagc gtcctgctga cgatcgaccc gaaggcgcag
     3241 cgggccgcgc tggacgggct ccaggacttg acgaggcagt tcggaccgac gaagggcgct
     3301 gtggtcgcgc tcgaccccac gaccggggcg atcctggcga tggtgaccca gccgtcgtac
     3361 gacccgaacc tcatcgccag ccacgacccg acgaaggcca ggcgcgcgtt caacgcggtc
     3421 gtgaacgcgc ccgaccagcg cggactgaac cggggcgcgc agtcgatcta cccgcccggc
     3481 tcgacgttca aactcgtgac gtcggcggcg gcactgagca acggctacac gccggactcg
     3541 acggtcaagg gcggcacggt gttcgacctg ccacagtcca gcgacacgat tcacaacgag
     3601 aacggcagcg actgcggcgg atcgcagatc accctgcggc acgcgctcga ggtgtcgtgc
     3661 aacgtcgcgt tcgccgacct cggtgtgaag ctcggggcgc agacgctgca ggagcaggcc
     3721 gagaagttcg gcttcaacga cgacgtcctc gacgagctgc ccgcggcgac aagcgtgttc
     3781 ccgaccgacc tcgacgagcc gcagaccgcg ctctcggcga tcggtcagta cgacgtggcc
     3841 gcctcgccgc tccagatggc gctggtcggg gcaggcatcg ccaacggcgg cgcggtgatg
     3901 aagccgtaca tcgtgcagca ggtgcgatcc cctgatctcg acgtactccg tgaggccgac
     3961 ccgcaggtcc tccaccaggc ggtctcggcc tcggtggcca gcgagctgac ccagatgatg
     4021 gtggacgtgg tccagtccgg tacggggacg aacacccaga tccctggtgt cacggtcgcg
     4081 ggcaagacgg ggacggcgaa ctccgccgag gatcgtgcgc cgtacgcctg gatggtgagc
     4141 ttcgcacccg cggacgaccc ccaagtggcg gttgcggtca tcgtcgaaca gaccggcgtc
     4201 agcaggagcg agatcagcgg caacgggctt gccggaccga tcgcccggtc ggtgatggag
     4261 gcggtgatca accgatgatg accgccgtcg gcaacggccg atcccgaccc ccggccagca
     4321 agccgacacc accgcggagg acgccctacg ccttgcgcgc gttgcagggc tccagcagcc
     4381 cagcgacgag tccggcagcc cgacaccggc gtcaggcctg gctgtccatc gccgcggccg
     4441 ccggggcggt cctcctcggc ctcggactcg cgtacgcctt cacccatggc ggcggtcccg
     4501 accagggcgc ggtcggccgc acggccggcc agacgttcac cggaccggtc gactccggca
     4561 agggcgaacg aacagcgccg ccgaccgcgc ccgccgcgcc ggtgaccctc accgtcgacc
     4621 cgtccgacta cgtgggtcga gacgccaagg acgtcgaggt cgagctcgag cgcctcggtt
     4681 tggctgtgca cgtggagcac atcgaagccg gcgagaaggg cactgtcgcc gcactcgcgc
     4741 cgacgggcgc gttggaggaa ggacagcagg tgacgttgtc ggtgtacaag ggaacgtcgg
     4801 ggcagcccga ccactcaggc gacttcggcc cagcgtcgga caagcaacgc gatcacgagg
     4861 aggactgacc ggtgaacatg gacaagacca tgatcggcca cggtcggtac cagatgggcg
     4921 cgctactcgg ccgaggcggc atggccgagg tacaccgggc tgaagacctg cgcctcggcc
     4981 gcccggtcgc gatcaagcga ctgcgcgtcg acctcgccag cgaccctacg ttccaggcgc
     5041 ggttccgccg ggaggcccag tcagcggcgt cgctcaacca cccgaccatc gtcgccgtct
     5101 acgacaccgg tgaggaggag gcaccagacg ggtccggcct cagcctgccc tacatcgtga
     5161 tggagctggt cgagggccgg acactgcgcg acatccttcg cgagggccgc cgcatcctgc
     5221 cggagcgcgc gctggagatc accagctcgg tgctggaggc gctcgactac agccaccggg
     5281 ccggcatcat ccaccgcgac atcaagccgg ccaacgtcat gctcacgccg tccggggacg
     5341 tgaaagtgat ggacttcggg atcgcgcgcg caatcgccga ctcgtcggcc acgatgaccc
     5401 agaccgccgc ggtggtcggt accgcgcagt acctgtcgcc ggagcaggct cgtggcgagc
     5461 aggtggatgc gcgcagcgac ctctactccg cgggatgtct gctgttcgag ctgctcaccg
     5521 gtcggccgcc gttcgtcggc gactcgccgg tgtcggtggc ctatcagcac gtgcgcgagg
     5581 acgccgtacc cccgtcagcg ttcgatcccg agctctcacc ggcgatcgac gcgatcacgt
     5641 tgaaggcgct ggcaaagtcc gtcgaccagc gttaccagag cgcttcggag atgcgggccg
     5701 acatcatgcg cgcgctcgag ggaaggtcgg tgctggcacc ggccgtggcg atggaggaga
     5761 cgcagcagtt cctcccgccg ccgccgaccg acagcacacc gacagcggtg acgccggcga
     5821 tggccaccga ggaccgcgag gagggtcgtc gcagggttgg cctctggatc ctgttggcgg
     5881 ctctggtcgt cgccgcactc gccgccggtc tgtactacgc cttcgccacc accggcgacg
     5941 gtgtcacgac gacgactgtc ccgaacgtgc agggcctgac gttggatcag gcggagaaga
     6001 agatcgagga cgccaacctc gtcgtcggcg acgagacgcc ggagaacagc gacacggtca
     6061 agaaggggct cgtccttcgc caaagcgaga caccgaacgc gaccgtcgac gagggcaccg
     6121 tcgtcgacct cacgtactcc gccgggcaga agaccgcgat cgtcccggac gtcaccgaca
     6181 tgaacctcac cgacgccacc gcagcgctgg aggaccgggg tttcaacgtc atcccgaagc
     6241 aggacaccga gagtcctgag gctcataacc aggtgacccg gaccgacccc gggccgagca
     6301 cgcgtttgcc ggtcggctcg aacgtcaccg tgttctactc gaccggcttc cgcgaggtgc
     6361 cgaacgtcgt gggccagacc gagcgcaacg ccacccacct gctgcagtcg ctgggcttcg
     6421 tcgtggttcc gtctgacatc cagtccgatc agcccgaggg tcaggtggtc gcacagaacc
     6481 cgccgggcga gagccgccag cccttcggct cgacggtcgt catccaggtg tcactcggac
     6541 ccaccgagac caccccgatc gagaccactc ccaccgagac caccccgacg gagacaaccc
     6601 ccaccgagac aaccaccgag accacgccca ccccgtgacc tgtgacgttg tcgcggtcgg
     6661 tcgaggtgtc actcgcggtg cgacacgccg acaaaagtga acgaaagttg ccacctcggc
     6721 gttgggcggg atgcggatca gtcggtgagg ggttgggcgt actcgagcgt gatcggctgc
     6781 ttgtacgcag ggacgcgcac gcgtgtctcg gtgcgtagat cccaacccag gttgtatggg
     6841 ggctcggtga aggcacgata gtcctgcacg tccggcgagt tcgtcagcgc gtcgtacatg
     6901 gccacttgat caccgaccgc gacgatccgg tacggcggcg catacgggac gccctgcaac
     6961 cgaacggtgt tgccgacaca cttgatgccg gtcgtgccga tgatgcgctg gccttgtagc
     7021 gaaatgcctt cggcgccgcc cgcccacagt gcgttgatga ccgcctgaag atcctgctga
     7081 tggacgacga gcaggttcgg gttggtcccg tcgggcacgt cctggtccag cggggcgtcg
     7141 tcgagcgtga cgacgaggcc cggtccggcc agccgctgca tgccggcggg agcggccagt
     7201 ctctcgagct tgcggttcag ctggttgacc tgcgggtcgt tgatcgccgc cgtcagctcg
     7261 tcgatgctgc gctggaccgt ttgtgcctgc acacgcaggt ccttgacgcg ctggctctgg
     7321 tcggcaacca caccggtcag gtcattgttg cgcccgccgc gcaggtcggt gccgcgcgca
     7381 ttgatcatgc tggtggtcgc cagcagaccc gcgcacgcac aggccagcgg aacggcgatc
     7441 cgccagccga cagatcggcg acggctcgtc cagagccacg cgaccggcga ccgacgggcc
     7501 ggcgagcgat ggctctgggg ggaaaggggt tcggacacgg actctcgggg ggactctcgg
     7561 gcgacgactg ttcgcgccaa gggtagtcgc cgcccgtgca gcagtcccaa tctgcgacgg
     7621 gttaccctga tcactcagcc gtttgcagca acaggagaag ccgtgcccga gtcccgatcc
     7681 cgcaagaagg ccgactacac cccacccacg tcgagcaaga agcccgcccg ggtcggtagt
     7741 cgccgctggg ttgccccggt catggtcggc tgctggctgt tcggtctggc ctggatcgtg
     7801 gtcttctacc tggcgcccga caccaagtac ctgcgcgacc tcggcaactg gaacctcgcc
     7861 gtcggcatgt tcttcatcgc gctcgggttc gtcttctcca ccaagtggga gtagccggcc
     7921 gaccgctggt cgccgtgctc ggccatctgc cgttcaccct gccgtcaccg cccgtactta
     7981 cacagatgta gttctccaca gcggttgtcc ccagtgtgga caacccgcct tcggcggtcg
     8041 agcttgtgga gacccggccc ttgatctcga caggctcgat cagcggggcc ggctcgatca
     8101 gcggggccgg ctcgatcagg gggcgtcagg tgagggcggc ggtgcgagcg agggctgcca
     8161 gcacgagtac gacgccgacg ccgatcaaca tgccggcatg gatcggtgtg cgctgactcc
     8221 tcggcgtgta cgcgaacccg gcgcccatca cgagcccggc gacaagacct ccgacgtgcg
     8281 cctgccacga cacgttcgac gcggtgaagc tgaacacgag gttcaggccg agcaggatga
     8341 ggagttgggt cacgtcccgg tgttgccgca gcaagatgac cagggatgcc ccgaagagcc
     8401 cgaacaccgc gcccgatgcg ccgagcgaga acgatcccgg tggcgcgagc cagtagatcg
     8461 cgaccgagcc ggcgagcccc gacagcaggt acagcagcag gtagcgccac cggcccaacg
     8521 ccacttcgag gaatgagccg aaaatccaca gccccaccat gttcagcagg atgtggagcg
     8581 gttgggtgtg gaggaaggtc gacgtgacca gctgccagta ggacccgtcg gccaccccgt
     8641 cgacgatgac gacctgtccg ttgctcaacc gggtcggccc gccctgcggg agcaggatca
     8701 gcgtccgggc gaacttgccg tccacgccgc cggtggccac cgccacgatc cagacgagga
     8761 cgttgatcgc gatcagactg atcgtcacga cgttcgagcc gctggcgacc cggccgccat
     8821 agaccgtacg caccgagcgc gactgcgtgg cacctcgttt cacgcagtcg gggcactgga
     8881 agcccaccga ggcctgccgc atgcagtcag gacagat
//
LOCUS       DATBFZ010000507        19340 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_3836296, whole genome shotgun sequence.
ACCESSION   DATBFZ010000507 DATBFZ010000000
VERSION     DATBFZ010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 19340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19340
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3836296"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3009
                     /locus_tag="VH419_15615"
     CDS             <1..3009
                     /locus_tag="VH419_15615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013069514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HEY7045098.1"
                     /translation="DLPVASPQVFGDQTDVPESGDWWDATYLMMWGSNVPVTRTPDAH
                     WMAEARYRGTKVVSVSPDYADNTKFADEWLPAQAGTDAALAMAMGHVILKENFVDREV
                     PFFTDYVRRYTDLPFLVRLESHGDGTAHVPGKFLMAADLPGEAAPEDSWKTVLLDDVT
                     GQPVVPNGSMGFRYADSGIGQWNLDLEGVTPRLSLYDHDHASVEVLLPAFDAPDGSGS
                     VVRRGVPAQRVGGHLVTTVFDLMLAQYGVARAALPGEWPEGYDDGSTPYTPAWQAEIT
                     SVPAQACIRIAREFAANSEESGGRSMVIMGAGICQWFHGDATYRAVLALLMLTGCQGR
                     NGGGWAHYVGQEKCRPITGWISLANALDWSRPPRTMIGTAYWYMHTDQWRNDGYSTDS
                     LASPLANGHLRGKHTADTVAQAARLGWMPFYPQFERNPLDLADEAGEAVERGAAPDTA
                     AYVASSLADGSLRTAIEDVDAPQNWPRTLVVWRSNLLGSSAKGNEYFLKHLLGTHSNV
                     MGEENPLAPRPTEVAWRDDAPEGKLDLLVSADFRMTSTTLLSDVVFPAATWYEKHDLS
                     STDMHPFVHAFSPAIDPPWEAKTDFETFHLLAEEVSRLARTHLGTRRDLVSVPLQHDT
                     PGETAQPGGRVDDWKVVGKVGTPGRNMPVFTVVERDYTAIADKIAAIGPLADSAGFTV
                     KNVTFRLDEQLARLARSNGVMLGGAADGRPAIDTDAKLAEAILTFSGTTNGSLAVQGF
                     KTLEQRVGKPLADLAEGSEEKRISFADTQVAPVPVITSPEWSGSETGGRRYAPFTVNI
                     ERLKPFHTLTGRMHFFLDHDWMQDLGEALPTYRPPLDMHRLFGEPTLGPDGAKQVTVR
                     YLTPHSKWSIHSEYQDNLLMLSLSRGGPTVWMSEADAKSIEVSDNDWVECVNANGVLV
                     ARAIVTHRMPEGVVYVYHVQERTIDVPKSEATGRRGGIHNSVTRLLVKPTHLIGGYAQ
                     LSYAFNYLGPTGNQRDMVTTIRKRSQTVEY"
     gene            3056..4732
                     /gene="narH"
                     /locus_tag="VH419_15620"
     CDS             3056..4732
                     /gene="narH"
                     /locus_tag="VH419_15620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HEY7045099.1"
                     /translation="MKVMAQMGMVMNLDKCIGCHTCSVTCKQAWTNRAGTEYVWFNNV
                     ETRPGQGYPRRYQDQETWRGGWTLTKRGRLKLKAGGKVQKLLSIFASPVQPSLADYYE
                     PWTYDYRNLVEAPLGDDFPVARPKSLITGEDTKVTWSANWDDDLGGAPEMGHLDPVVE
                     QVRRESEDRIRFELEQTFMFFLPRICEHCLNPSCMASCPSGAIYKRAEDGIVLVDQDR
                     CRGWRQCITGCPYKKIYFNHKSGKAEKCTFCYPRIEVGRPTVCSETCVGRLRYLGLFL
                     YDADRVTEAASTRDEGELYQAQLDLLLDPDDPAVVAAARADGIPEDWLAAARRSPVFA
                     LAKRYGVALPLHPEYRTMPMVWYVPPLSPVVDLLREQGHDAENQSTLFGAIGSLRIPV
                     EYLAALFTAGRTDIVTDVLQKLAAMRSYMRDITLGRAADPRIPHSVGMTEESMYAMYR
                     LMAIAKYDERYVIPQAHVEQAHELEEIGCSLDFEGGPGMYESGPYGEASGRPAPVAVE
                     TFNALRQRQRSDEPTESHLQGRVNLLNWDGNGTPEGLFPDKTAGSSSEVS"
     gene            4729..5373
                     /gene="narJ"
                     /locus_tag="VH419_15625"
     CDS             4729..5373
                     /gene="narJ"
                     /locus_tag="VH419_15625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HEY7045100.1"
                     /translation="MTVRQGHDKAEHRVVHQVAAWCLDYPDEALARQLPTLRGALAEQ
                     PEGRPVRLLRTFLDHVSATPLEALQQHYVKVFDLSRKHALHLSYWTDGDTRRRGEVLA
                     AFKARYRDTGFLVDTGGELPDFLPMVLEFAAVADPAAGAELLQQYRPSLELLRLGLLE
                     DGTPYADVVTAVCETLPGPSPADRAAVMAMAGAGPPVESVGLEPYGVRVLPVQG"
     gene            5384..6133
                     /gene="narI"
                     /locus_tag="VH419_15630"
     CDS             5384..6133
                     /gene="narI"
                     /locus_tag="VH419_15630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HEY7045101.1"
                     /translation="MRMDTVLWGVLPYLMLAVLIGGTVWRYRYDKFGWTTRSSQLYES
                     RLLRIGSPLFHFGILLVLVGHIGGLVIPESWTSAFGVSEGLYHVNAVLFGGLAGICTL
                     VGILILIYRRRTTGPVFMATTKNDKTMYVVLVGAIVLGLWTTLASVGAGSEAHNYRES
                     VSPWFRSLFVLQPDVDSMAEAPLSFHIHTLIGMLLFAIWPFTRLVHAFTAPLHYLFRP
                     YIVYRSRDLQAAGARPSRRGWSDVGTPDRKA"
     gene            6135..7364
                     /locus_tag="VH419_15635"
     CDS             6135..7364
                     /locus_tag="VH419_15635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY7045102.1"
                     /translation="MEAELSQTQPRTDIRGGQAKNLALATLAFAISFWAWNLVAPLGV
                     RYTEQLGLTSNQKSLLVAIPVLVGSLGRILAGALTDRYGGRRMFPLLMLLATPFVLLI
                     AYAGNQDSYGLLLVFGFFLGIAGTTFAVGIPFVNAWYEPARRGFATGVFGAGMGGTAL
                     SAFFTPRFVNWFGYTATHVIVAVALAATAVLCWFTMRDSPAWSPNIDPFMPKLVAALK
                     LPVTWQMSFLYAVAFGGFVAFSTYLPTYLKDIYDLDLTGAGTRTAGFAVAAVVARPIG
                     GFLSDRVGPRVIVAISLAGTTVLAVVEALEPPLEVPAGLSFVLLAGFLGLGTGGVFAW
                     VARQAPPERVGSVTGVVGAAGGLGGFFPPLVMGATYEVLWDGYGLGLSLLAVTAAIAL
                     AFTLLDIKPARHTTPAD"
     gene            complement(7369..7983)
                     /locus_tag="VH419_15640"
     CDS             complement(7369..7983)
                     /locus_tag="VH419_15640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020385100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEY7045103.1"
                     /translation="MTHTTCHVTISLDGFIAGPSQSRDDPIGVGGLRMHEWHFKADDP
                     GFEQDARMRDDLLRERGAFVMGRNMFGPIRGEWDEEWNGWWGAEPPYHAPVFVLTHHA
                     RDSVEMQGGTTFHFVTDGFDSAYAQACAAAGDKEVTIAGGASTVRQALAAGALDEIWL
                     DIAPVLLGSGERLFDGVEDPGLDPVEVVASPRATHIHYRIGSEA"
     gene            complement(8014..8373)
                     /gene="arsC"
                     /locus_tag="VH419_15645"
     CDS             complement(8014..8373)
                     /gene="arsC"
                     /locus_tag="VH419_15645"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00014.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HEY7045104.1"
                     /translation="MTDEDVVIWYNPRCSKCRGAEDLLAAYGVSSRQLRYLEDPPPRE
                     EIVRVLGLLDATGPREMMRTKEPRYTELGLDGASDNELIDAMAAHPELIERPIVIRED
                     RAVIARPPELLLSLLDR"
     gene            8659..9804
                     /locus_tag="VH419_15650"
     CDS             8659..9804
                     /locus_tag="VH419_15650"
                     /inference="COORDINATES: protein motif:HMM:NF024810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HEY7045105.1"
                     /translation="MARQTTILSLVLVVGGWLAACTGPEATDEIQAGRSTAASGLGDD
                     GYQAIQLLQPRAAHNATRLRDGRVLLTGGCTLPGCGGFEEARVTELFDPQTRSLAAGP
                     EMLYARASGTATLLADGRVLLTGGYPGEGEAPQDTAEVYDPEAGEFEQVGSMTTARAE
                     QTATLTSDGRVLIAGGRDADDRALQSTEWFDPDTDTFAPGPPLDAPRTAHVALVADDQ
                     VVLVGGTAGDRALASTSVLTDSGWRAGPRLATPRVKLAAVTLDDQRIFVVGGSESIEG
                     RHQLRTTELVNLVRDVVRPGPLLSEGEYKLDGAVVKLHDGRVVIAAGDRVNIFNPRTN
                     RISVLPGPTMGERSFVTATPLTSNEILVAGGYDAFIEPTDRAWLIRV"
     gene            9865..10608
                     /locus_tag="VH419_15655"
     CDS             9865..10608
                     /locus_tag="VH419_15655"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020388874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY7045106.1"
                     /translation="MRQDEIWDVDAAARYDTPGTGMFAPEVLEPTVNRLVELSAGGDV
                     LEFAIGTGRVAVPLSERGISVTGIELSQPMTDQLRTKVDADTIPVVEGDMASAQAGGV
                     YTLVYLVFNTISNLQTQAEQVACFRNAARHLAPGGRFVIELWVPDLRRLPPGQAATVW
                     QCDAEYIALDTYDVVQQHVVSHHFHLSDGEEARLFRSPHRYIWPAELDLMAQLAGFEL
                     ESRHADWVGSEFTAESRSHVSVYRLPSAL"
     gene            complement(10724..11506)
                     /locus_tag="VH419_15660"
     CDS             complement(10724..11506)
                     /locus_tag="VH419_15660"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY7045107.1"
                     /translation="MSGLPSGTVTFVFSDIEGSTTLLKALGDRYAEVLSGHRKLMRES
                     FAPRGGVEIDTQGDAFFFAFPRARDAVSAAVEAQRVHASHDWPNGSVVRVRLGLHTGE
                     PAVGSEGYLGIDVVRAARLCTLGRGGQVLLSETTRALVGSSLPDGVSVHTLGERQLKD
                     IDEPERVFELDIEGAPAPEAVATSAKPAEAVSEPAVDEPVPDDLGPVDMDDFGTRLSR
                     RIQEHVRRKIEEPLERMFSEVDGLADKAAKHPESISEKLDEE"
     gene            11620..11802
                     /locus_tag="VH419_15665"
     CDS             11620..11802
                     /locus_tag="VH419_15665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7045108.1"
                     /translation="MSSKAGAAAGSGAIYGLGIFGALVYYWQEAERAWEYVYAVFQAF
                     FWPAYMVYSAFSALHA"
     gene            11901..12779
                     /locus_tag="VH419_15670"
     CDS             11901..12779
                     /locus_tag="VH419_15670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7045109.1"
                     /translation="MRIRLLGVAIVTAVSSGCGLALSPSTDSSTVVVEAHPVPHPYAG
                     PMSVQPDTSDNATVLERSGAAGRALECDYEPYDGGGGDYDSGLESVQDSPAKALENFL
                     EEELFLQLPKTGYQLEREDDGRALLSYDVGQRTKIAFIAADGIRDYEDDEGWGIETWA
                     ECDPAELPAPVTEALGIGVWQDRSGRRVPVAQIRSFQGPEHCSWQDVTFLELGPEHHA
                     DEYVGHPTPDLADWLKTTYTTNPPWPPGVTDTGFRQDGRELWLGPEHDAAYLVSRGDP
                     TNVERWPAAKQPIGCD"
     gene            12800..14068
                     /gene="kynU"
                     /locus_tag="VH419_15675"
     CDS             12800..14068
                     /gene="kynU"
                     /locus_tag="VH419_15675"
                     /EC_number="3.7.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030429 - kynureninase activity [Evidence
                     IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HEY7045110.1"
                     /translation="MSSGTETTRAYAEQLDRADPLASYRDEFVIPDDDLVYLDGNSLG
                     RLPKSSQERVKHVLDDDWGGQLIRSWEDRWMHLPTQIGDLIGTGLLGAAPGEVVVGDS
                     TTVSLYKAMSALLDAQSDRRAVIIERDNFPSDRYLVESLAKQRDLEVRWIDETGYEGV
                     SSDHVKPLLDNDVALVSLSHVDYRSAAIVDMASITAAVHEVGALTLWDLCHSVGSIPI
                     DLHRAGADVAVGCTYKYLNSGPGAPAFTYVRTDLQPRLSQPIWGWWSRAEMFDMAQGY
                     QPYADMRSWLVGTPSVLSLVAIEPSVRLILDAGMDALRAKSLLLTAYAVDLFDSWLAP
                     LGAGLASPREPERRGSHVTVTVPDAKVLCAQLTRRGVLPDFRRPDGIRIGLAPLTTRF
                     VDVYDGLATLRGLLVSGVTRSSVDVAQREP"
     gene            14192..16255
                     /locus_tag="VH419_15680"
     CDS             14192..16255
                     /locus_tag="VH419_15680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012923170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HEY7045111.1"
                     /translation="MKRLATIGAIAAVTAAGLSAAPSGAAIATAPAASRQLVTKADPH
                     FTPKERAAVLREARADARDTARTLRLPASQSLHPTDALADTNGAVHIRYDRTYRGLRV
                     IGGDLVVHQRPSGKVKSVDYASQANVALRSIHPRTGAPAKRSHIVVFAARHRAVLAWE
                     THTIGTTRRGGPISRLTYTNANTGRRITSWSTVDTADGTGNSLYSGTVPLKTVLSGST
                     YQLIDQTRGSSAIYDANNSTGTGRGTLFTDSDNVWGNGSTTSRQSAAVDAAYGAANTW
                     DFYKNTFGRNGIRNDGVAAYSRVHYGRNYENAFWDDSCFCMTYGDGGSTFSPLVSLDV
                     AGHEMSHGVTSNTAGLVYSGESGGLNESTSDIFGTMVEFTANNASDPGDYYIGEEIAR
                     DGTYLRRMDHPSLDGASADCWSSGVGNLNVHYSSGVGNHFFYLLAEGSGAKTIGGRSH
                     NSPTCNGSSVTGIGRDAAARIWYRALTTYMTSTTNYKGARDAAIKAARDLYGATSTQC
                     NTVVSAMNAVSVPVGAETCSGSNPPPPGGNLLLNPGFESGNVNWSATSGVIGTGSGYQ
                     PRTGAYNAWLDGYGTTHTDTLSQTITIPSGKSTASLGFYLWITTQERGSTVYDTLRVQ
                     AIRNGVTTTLATYSNANAGSGYVLRTLSLNSYIGQTVTLRFTGTEDSSLATSFRIDDT
                     SVTTT"
     gene            16420..18027
                     /locus_tag="VH419_15685"
     CDS             16420..18027
                     /locus_tag="VH419_15685"
                     /EC_number="1.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinic semialdehyde dehydrogenase"
                     /protein_id="HEY7045112.1"
                     /translation="MTPRPGPDVDAPADPEHDPRAAYAVDPGLVRRLATRIDAGSGRT
                     ETTYAPFTGQPIATLTVSTPDDVAAAVQQARQAQRAWARVPVELRQRIFLRFHDLVLD
                     RQDELLDLIQWESGKARSHAFEEVAHVAMTARYYARTGGKQLAGGRRYGILPLLTRVD
                     VHQVPLGVVGVIGPWNYPLTMAMADGLAALYAGNAVVLKPDAQTPLSALLGVDLLSEA
                     GVPPDAWRVVYGDGPVIGGAILDHVDYVSFTGSTSIGRSIATRAAARLIGASLELGGK
                     NPILVLRDADLERAAEGAVRACFSSAGQLCVSMERMYVADQIYDRFLDRFVRRTEAMR
                     LSPALDYSADMGSLVSQRQLDTVTAHVQDAVRQGAMVVTGGHARPDVGPLFHEPTVLA
                     GVTPTMRCFAEETFGPVVSVYRFSDEQEAVARANDGVFGLNASIYTRDAARGRRLARQ
                     LRCGSVNVNEGYAASFGSLDSPMGGMRDSGLGRRQGPEGLLRYTETQTVATQRGLPFR
                     PVLGMSQQRYAKTFTSTLRLLKELRRP"
     gene            18024..>19340
                     /locus_tag="VH419_15690"
     CDS             18024..>19340
                     /locus_tag="VH419_15690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007078554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEY7045113.1"
                     /translation="MTHDYDVIVVGSGFGGSVAALRLTEKGYRVGVLEAGARFDETSY
                     PATSWDLRRFLFAPALGCYGIQRIDVLKNCVILSGAGVGGGSLVYANTLYEPLDAFYR
                     DPSWSHITDWRTELAPYFDQARRMLGVTTNPTMTPSDVVMKQVADELGVGATFRRTPV
                     GVLFGEPGADVPDPYFGGAGPDRKACIECGECMTGCRHHAKNTLTKNYLHLAEQAGAV
                     VHPLTTVTGIRPLAVGGYRVATRPTALHGRRARRASRAFTAEQVVLAASTLGTQRLLH
                     RMRDEGLLPRLSPRLGHLTRTNSESILGAVASGGDVDFSRGVAITSSFHPDDHTHIEP
                     VRYGKGSNAMSLLQTVLTDDDGGVARWRVWLREMWRQRRSLASLYDVRHWSERTVIAL
                     VMQTHDNSITTYTKRSGLTRRRRLTSRHGHGAPNPTWIPVGNDAVRR"
BASE COUNT         3115 a         6568 c         6530 g         3127 t
ORIGIN      
        1 gacctccccg tcgcgagccc gcaggtcttc ggcgaccaga ccgacgtgcc cgagtccggc
       61 gactggtggg acgccacgta cctcatgatg tggggctcca acgtcccggt cacgcgcacg
      121 cccgacgcgc actggatggc agaggcccgg taccgcggca cgaaggtggt cagcgtcagc
      181 ccggactacg ccgacaacac caagttcgcc gacgagtggc tccccgcgca ggcgggtacg
      241 gacgccgcgc tggcgatggc gatggggcac gtgatcctca aggagaactt cgtcgaccgt
      301 gaggtgccgt tcttcaccga ctacgtacgc cgctacaccg acctgccgtt cctcgtccga
      361 ctcgagtcgc acggggacgg gaccgcgcac gtccccggca agttcctcat ggccgccgac
      421 ctgccaggcg aggccgctcc ggaggactcg tggaagacgg tcctgctcga cgacgtgacg
      481 ggccagccgg tcgtgccgaa cggctcgatg ggcttccgct acgccgactc cggaatcggg
      541 cagtggaacc tcgacctcga gggtgtcacg ccgcggttga gcttgtacga ccacgaccac
      601 gccagtgtcg aggtgctgct gccggcgttc gacgcgccgg acgggagtgg ttcggtcgta
      661 cgccgtggcg ttcccgcgca acgggtcggc ggccacctcg tgaccacggt gttcgacttg
      721 atgctggcgc agtacggcgt cgcgcgcgcg gctctgccag gggagtggcc cgaaggctac
      781 gacgacggtt cgacgccgta cacgccggcg tggcaggccg agatcacaag tgttccggcg
      841 caggcgtgca tccggatcgc ccgcgagttc gccgcgaact ccgaggagtc cggtggccgc
      901 tcgatggtca tcatgggggc ggggatctgc cagtggttcc atggcgacgc gacgtaccga
      961 gcggtccttg ccctgctcat gctgaccggc tgccagggcc gtaacggcgg cggctgggcg
     1021 cactacgtcg gacaggagaa gtgtcggccg atcaccggct ggatctccct ggccaacgcg
     1081 ctcgactgga gccggccgcc acggacgatg atcgggaccg cctactggta catgcacacc
     1141 gaccagtggc gcaacgacgg ctactcgacg gactccctcg cctccccgct ggccaacggc
     1201 catctccgcg ggaagcacac cgccgacacg gtggcgcagg cggcacggct cggctggatg
     1261 ccgttctacc cgcagttcga gcgcaacccg ctcgacctgg ctgacgaggc cggcgaggcg
     1321 gtcgaacgcg gcgccgcgcc ggacacagcg gcgtacgtcg cctcgtcgct cgcggacggg
     1381 tcgctgcgca ccgccatcga ggacgtcgat gcgccacaga actggccgcg caccctcgtc
     1441 gtgtggcggt cgaacctgct cggctcgtca gcgaaaggca acgagtactt cctcaagcat
     1501 ctgctcggca cccactccaa cgtcatgggc gaggagaacc cgctggcgcc gcgaccgacg
     1561 gaggtggcgt ggcgcgatga tgcgcccgag ggcaagctcg atctgctggt gagcgctgac
     1621 ttccggatga cgtcgacgac gctgctctcg gacgtggtgt tcccggctgc gacgtggtac
     1681 gagaagcacg acctctcgtc gacggacatg catccgttcg tgcatgcgtt cagtccggcg
     1741 atcgacccgc cgtgggaggc gaagaccgac ttcgagacct tccacctgct cgccgaggaa
     1801 gtctcccgac tggcgcggac gcacctgggc acgcgccgtg acctcgtgag tgttccgttg
     1861 cagcacgaca cgccgggcga gacagcgcaa ccaggtggcc gggtggacga ctggaaggtg
     1921 gtcgggaagg tcggtacgcc aggtcgcaac atgccggtgt tcaccgtcgt cgagcgtgac
     1981 tacacggcga tcgcggacaa gatcgccgcc atcggcccgc tggccgactc ggccggtttc
     2041 accgtcaaga acgtcacgtt ccggctcgac gagcagcttg cccggctggc ccggtccaac
     2101 ggtgtgatgc tcggcggcgc tgccgacgga cgcccggcga tcgacaccga cgccaagctg
     2161 gcagaggcga tcctgacgtt ctcgggcacc acgaacggct cgcttgcggt gcagggcttc
     2221 aagacgctcg agcaacgggt cggcaagccg ctcgcggacc tggccgaggg ctcggaggag
     2281 aagcggatct cgttcgccga cacccaggtc gcgccggtgc cggtcatcac gtcgccggag
     2341 tggtcgggct cggagaccgg tggccgacgc tatgcgccgt tcaccgtcaa catcgagcgg
     2401 ctcaagccgt tccacaccct caccggccgg atgcacttct tcctcgatca cgactggatg
     2461 caggacctcg gcgaggcgct gccgacgtac cgtcctccgc tcgacatgca ccggctgttc
     2521 ggcgagccga cgctcgggcc cgacggcgcg aagcaggtca cggtccgcta cctcacgccg
     2581 cactcgaagt ggtcgatcca ctccgagtac caggacaacc tgctgatgct ctcgctgtcc
     2641 cgcggtgggc ccacggtctg gatgagcgag gccgacgcga agtcgatcga ggtgtccgac
     2701 aacgactggg tggagtgcgt caacgccaac ggtgtcctgg ttgcccgcgc gatcgtgacc
     2761 caccggatgc cggagggagt cgtgtacgtc taccacgtgc aggagcgcac gatcgacgta
     2821 ccgaagtcgg aggcgaccgg gcggcgcggc ggcatccaca actcggtgac gcggctgctg
     2881 gtgaagccga cgcacctgat cggtgggtac gcccagctgt cctacgcgtt caactatctg
     2941 ggacccacgg ggaaccaacg ggacatggtg acgacgatcc ggaaacggtc ccagacggtc
     3001 gagtactagt cagcggaacg atcccagagc ccagaccggg aagggaggta cggcgatgaa
     3061 ggtcatggcg cagatgggca tggtcatgaa cctcgacaag tgcatcggct gccacacctg
     3121 ctcggtgacg tgcaagcagg cgtggaccaa ccgcgcgggc acggaatacg tctggttcaa
     3181 caacgtggag acccgccccg gtcagggcta cccccggcgt taccaggacc aggagacctg
     3241 gcgcggcggt tggacgctca ccaagcgtgg ccggttgaag ctcaaggccg gtgggaaggt
     3301 ccagaagctg ctgagcatct tcgccagccc ggtgcagccg agcctcgccg actactacga
     3361 gccgtggacc tacgactacc gcaacctcgt cgaggcgccg ctcggggacg acttcccggt
     3421 cgcgcgaccc aagtcgctca tcaccggcga ggacacgaag gtcacctggt ctgccaactg
     3481 ggacgacgac ctcggcggcg caccggagat ggggcatctc gacccggtgg tcgaacaggt
     3541 gagacgtgag tcggaggacc gcatcaggtt cgagctcgag cagacgttca tgttcttcct
     3601 tccgcggatc tgcgagcact gcctgaaccc gtcgtgcatg gcctcctgcc cgtccggcgc
     3661 gatctacaag cgcgccgagg acggcattgt cctcgtggac caggatcgct gtcgcggctg
     3721 gcggcagtgc atcaccggct gcccgtacaa gaagatctac ttcaaccaca agtccggcaa
     3781 ggccgagaag tgcacgttct gctacccgcg catcgaggtg ggccggccga ccgtgtgctc
     3841 ggagacctgc gtgggccgac tgcgctacct gggtctgttc ctgtatgacg cggaccgggt
     3901 cacggaggcg gcgtcaacgc gtgacgaagg cgagctctac caggcgcagc tcgacctgct
     3961 gctcgaccca gacgaccccg ccgtcgtggc agccgcccgg gcggacggga taccggagga
     4021 ctggctggct gctgcccggc gctcgccggt cttcgcgctg gcgaagcggt acggcgtcgc
     4081 gctgccactg cacccggagt accgcacgat gccgatggtc tggtacgtgc cgccgctgtc
     4141 tcccgtcgtc gatctgctga gggagcaggg tcacgacgcc gagaaccagt ccaccttgtt
     4201 cggtgcgatc ggctcgttgc ggatcccggt ggagtatctc gccgcgctct tcacggccgg
     4261 ccgcacggac atcgtgacgg acgttctgca gaagctcgcc gccatgcggt cgtacatgcg
     4321 cgacatcacg ctgggtcggg cggcggaccc gcgcatcccg cactcggtcg gcatgaccga
     4381 ggaatccatg tacgcgatgt atcggctgat ggcgatcgcc aagtacgacg agcgctacgt
     4441 catcccgcaa gcgcacgtgg aacaggcgca cgagctggag gagatcggct gctcgctcga
     4501 cttcgagggc ggaccgggta tgtacgagtc cggcccgtac ggcgaggcgt ccggccggcc
     4561 cgccccggtg gcggtcgaga cgttcaacgc actgcggcag cgacagcgct ccgacgagcc
     4621 gacggagtct cacctgcagg gccgggtcaa cctgttgaac tgggacggca acggaacgcc
     4681 cgaagggctg ttcccggaca agaccgccgg ctcgtcgagc gaggtctcat gaccgtccgt
     4741 caaggtcacg acaaggccga gcatcgcgtc gttcatcagg tggcagcgtg gtgtctggac
     4801 tacccggacg aggcgttggc gcggcagctg cccacgctgc gcggcgcgtt ggcggagcag
     4861 cccgagggca ggccggtccg gctgctcagg accttcctcg accacgtgag tgcgacgccg
     4921 ctcgaggcgt tgcagcagca ctacgtgaag gtgttcgacc tctcccgcaa gcatgccctc
     4981 cacctgtcct actggaccga cggtgacacc cgccgccgcg gcgaggtgct ggcggcgttc
     5041 aaagcccgct accgggacac cggtttcctc gtcgacaccg ggggtgagct accggacttc
     5101 ctcccgatgg tgctggagtt cgctgccgtc gccgacccgg cggccggagc cgagctcctg
     5161 cagcagtatc ggccgagcct ggaactgctc cgactcggac tgctcgaaga cgggacgccg
     5221 tacgccgacg tggtcaccgc cgtctgcgag acgctccctg gcccgtcacc ggccgaccgc
     5281 gctgcggtga tggcgatggc cggtgctggc ccaccggtcg agtccgtcgg cctggagccg
     5341 tacggcgtac gcgtgctgcc ggtgcaaggg tgatgaggag gcgctgagga tggacacggt
     5401 gctgtgggga gtccttccct acctcatgct cgccgtgctc atcggcggga cggtgtggcg
     5461 ctaccggtac gacaagttcg gctggaccac acggtcgtcg cagctgtacg agtcgcggct
     5521 gctgcgaatc gggtcgccgc tgttccactt cggcatcctg ctggtgctcg tcggccacat
     5581 cggcggcctc gtcataccgg aatcctggac gtcggcgttc ggcgtcagcg aggggctgta
     5641 ccacgtcaac gcggtgctct tcggtggtct cgccggcatc tgcacgctgg tcgggatcct
     5701 gatcctgatc taccgcagac ggacgacggg gccggtgttc atggcgacga cgaagaacga
     5761 caagaccatg tacgtcgtcc tcgtcggagc gatcgtgctg ggtctctgga cgacgctggc
     5821 gagtgtcggc gcgggcagcg aggcgcacaa ctaccgagag agtgtctccc cgtggttccg
     5881 gtcgctgttc gtgctgcagc cggacgtcga ctcgatggcg gaggcgccgt tgtcgttcca
     5941 catccacacg ctcatcggga tgctgctgtt cgcgatctgg ccgttcaccc ggctcgtgca
     6001 cgcgttcact gcgccgctcc actacctgtt ccggccgtac atcgtctacc gcagccgcga
     6061 cctccaggcc gccggcgctc gtccatcccg ccgcggctgg tcggacgttg gaacaccgga
     6121 caggaaggcc tgacgtggaa gctgaactgt cgcagaccca accgcgcacc gacatccggg
     6181 gcggacaggc gaagaacctg gcgctggcca cgctggcgtt cgcgatcagt ttctgggcat
     6241 ggaacctcgt ggctccgctc ggtgtccgct acaccgagca gctcgggctg acgagcaacc
     6301 agaagtcgct gctggtcgcc atcccggtgc tcgtcgggtc gctcggccgg attcttgccg
     6361 gcgcgttgac cgaccggtac ggcggccggc ggatgtttcc gctgctgatg ctccttgcga
     6421 cgccgttcgt gctgctcatc gcgtacgccg ggaaccagga ctcgtacggc ctgttgctcg
     6481 tcttcggctt cttcctcggc atcgccggta cgacgttcgc ggtcgggatc cccttcgtca
     6541 acgcgtggta cgagcctgcc cgccgcggct ttgccaccgg ggtcttcggc gccggcatgg
     6601 gcggtaccgc cctgtcggca ttcttcacgc ctcggttcgt caactggttc ggctacacgg
     6661 ccacgcacgt catcgtcgcg gtggctcttg cggcgaccgc cgtcctctgc tggttcacga
     6721 tgcgggactc gcccgcctgg tcacccaaca tcgacccgtt catgccgaag ctggtggcgg
     6781 cgctgaagct gcctgtgacc tggcagatgt cgttcctgta cgccgtggcg ttcggcggct
     6841 tcgtcgcatt ctcgacgtac ttgccgacct acctgaagga catctacgac ctcgacctga
     6901 ccggagcggg gacgcgcacc gcgggcttcg cggtggccgc cgtcgtcgcg cgcccgatcg
     6961 gcggcttcct gtccgaccgg gtcggaccgc gtgtgatcgt ggcgatctcg cttgccggca
     7021 cgacggtgct ggcggtcgtc gaggcgctgg agccgccgtt ggaggtgccg gccggattgt
     7081 ccttcgtgct gctggcgggt ttcctcggcc tcggcacggg cggcgtcttc gcctgggtgg
     7141 cgcgacaggc tcctcccgag cgagtcggca gcgtcaccgg tgtcgtcggt gcagccggtg
     7201 gcctgggcgg gttcttccca ccgctggtga tgggcgcgac gtacgaggtg ctgtgggacg
     7261 gctacgggct cggactcagc ttgctcgccg tgacggcagc gatcgcgctc gcgttcaccc
     7321 tgctcgacat caagccggcg aggcacacga cccccgccga ctgagccctc acgcctcgct
     7381 gccgatgcgg tagtggatgt gggtggcacg cggcgacgcg accacctcga ccgggtcgag
     7441 tccgggatcc tcgacgccgt cgaagagccg ctcgccgctg ccgagcagaa cgggtgcgat
     7501 gtcgagccag atctcgtcga gtgcaccggc cgcgagtgcc tgccgcaccg tcgaggcgcc
     7561 acccgcgatg gtgacttcct tgtcgccggc cgccgcgcac gcctgcgcgt aggcggaatc
     7621 gaagccgtcg gtgacgaagt ggaacgtcgt cccgccctgc atctcgaccg agtcgcgcgc
     7681 atggtgggtg agcacgaaga ccggcgcgtg gtacggcggc tccgcacccc accagccgtt
     7741 ccactcctcg tcccactcac cgcgaatcgg gccgaacatg ttccggccca tgacgaacgc
     7801 tccgcgctct ctcagcaagt cgtcgcgcat ccgggcgtcc tgctcgaagc cgggatcgtc
     7861 ggccttgaag tgccactcgt gcatccgcag cccgccgact ccgatcgggt catcccgact
     7921 ctgcgagggt ccggcgatga agccgtcgag agagatggtc acgtgacacg tcgtgtgggt
     7981 catggttgca tcgaccttcc gtggcgtcgg aactcatcgg tccagtagcg acagcaacag
     8041 ttcgggcggt cgcgcgatga cagctcggtc ctcgcggatg acgatggggc gctcgatgag
     8101 ttcggggtgg gctgccatcg cgtcgatcag ctcgttgtcg gatgcgccgt cgagcccgag
     8161 ctcggtgtac cgcggctctt tcgtccgcat catctcccga ggaccggtcg cgtcgaggag
     8221 gcccaggacc cggacgatct cctcccgcgg tggcgggtct tcgaggtacc gcaactgcct
     8281 ggacgagacg ccgtacgccg ccagcaggtc ttcagcgccc cggcacttcg agcaccgcgg
     8341 gttgtaccag atcacgacgt cttcatcagt cacgcgcaca ccctactggc gcatcgcagg
     8401 ttcacgatga cggcagcgag gcacaggcgg ttttgtcgac ctcggcgatc gagcttgtcg
     8461 agacccggtc ccggcggagt ccttgatctc gacaagctcg atcagcgggt tactcgacaa
     8521 gctcgatcag cgggttactc ggcaagctcg atcagcggcc acctcggcag gggagaccgt
     8581 cgcttgaagg aagtcggctc ggcaccgcac agttggtgtg tggtccagtg gagggcttcg
     8641 gaaccgacag ggcgagcgat ggcccggcag acaacgatcc tttccctcgt cctcgtcgtc
     8701 ggcgggtggc tcgctgcctg caccggtccg gaagcaaccg acgagatcca ggcgggtcga
     8761 tcgacagccg ccagcggact gggtgacgac ggctaccagg cgatccagct gctgcagccg
     8821 agagcggcgc acaacgcgac ccggcttcga gacggtcgtg tcctcctgac gggtggctgc
     8881 acgttgccgg gctgcggtgg gttcgaagag gcccgagtca cggagctgtt cgatccccag
     8941 accaggtcgt tggcggcggg tccggagatg ctgtacgcgc gcgccagcgg gaccgcaaca
     9001 ctgttggcag atggccgcgt cctgctgacg ggcggctacc cgggcgaagg cgaggcgcca
     9061 caggacacgg ccgaggtcta cgaccccgag gccggcgaat tcgaacaggt cggctcgatg
     9121 acaacggcgc gggccgagca gacggcgacc ttgacgtcag atgggcgggt gctgatcgcg
     9181 ggtggccgag atgcggacga tcgggcgctg caatcgaccg agtggttcga tcccgacacc
     9241 gacacgttcg cgccaggtcc gccactcgac gcgccgcgca cggcccatgt cgcgctggtc
     9301 gcggacgacc aggttgtgct cgtcggcggc accgcgggcg atcgagctct tgcgagcacg
     9361 tcggtgctga ccgactcggg atggcgagcg ggtcctcggc tggcgacccc tcgtgtcaag
     9421 ctcgccgcag tcacgctcga cgaccaacgg atcttcgtcg tcggcggctc ggagtcgatc
     9481 gaaggacgac accagctccg gacgaccgag ctggtgaacc ttgtgcgcga cgtcgtacga
     9541 cctggtccgc tgctctccga gggggagtac aagctcgacg gcgccgtcgt caagctccac
     9601 gacggccggg tcgtgatcgc agcgggcgac cgggtcaaca tcttcaaccc acgtaccaac
     9661 cggatcagcg tgttgcccgg tccgaccatg ggcgagaggt ccttcgtcac cgcgacgccg
     9721 ctcacgagca acgagatcct tgtcgcaggt gggtatgacg ccttcatcga gccgacggat
     9781 cgtgcctggc tgatccgggt ctgactggac ccagctctga aaagggttgg acgcgatcgc
     9841 ggttccgttg gcatggtcgt cgccatgcgt caggacgaga tatgggacgt cgatgccgcc
     9901 gcgcgctatg acacgcctgg aacgggcatg ttcgcgcccg aagtgctcga gcctacggtc
     9961 aaccgtctcg tcgagctgtc cgctggtggc gacgtgctcg agttcgcgat cgggaccggt
    10021 cgtgtcgccg tcccgttgtc cgagcgcggc atcagcgtca ccgggatcga gctgtcccaa
    10081 ccgatgaccg accaactccg caccaaggtc gacgccgaca cgatcccggt cgtcgagggc
    10141 gacatggcga gcgcgcaagc cggtggcgtc tacacgttgg tctacctcgt cttcaacacg
    10201 atctccaacc tgcagacgca ggccgagcag gtggcgtgtt tccgtaacgc cgcccgccac
    10261 ctcgcgccgg gcggccggtt cgtcatcgag ctgtgggtac cggacctgcg caggctgcca
    10321 cctggtcaag ctgccaccgt ctggcagtgc gatgcggagt acatcgctct ggacacgtac
    10381 gacgtcgtgc agcaacacgt tgtctctcac cacttccacc tcagcgacgg tgaggaggcc
    10441 cggctcttcc gcagtccaca ccgctacatc tggccggcag agctcgacct gatggcgcag
    10501 ctggcgggct tcgagctcga aagcaggcat gctgactggg tcggatccga gttcaccgcc
    10561 gagtcgcggt cgcacgtgtc ggtgtaccga ctgccatcag cgctttgagg ttttcgatgt
    10621 ttacagccca aaaggtgtgt aaacttcgca aacctccgat aggcacggtg ggtttcggat
    10681 gtgaacatcg caaactgcgg cggctggcgt ccggcttccc ctgctattcc tcgtcgagct
    10741 tctcgctgat cgactccggg tgcttggcgg ccttgtcagc caggccgtcg acctcggaga
    10801 acatgcgctc gagcggctct tcgatcttcc gccgcacgtg ttcttggatt cgccgggaga
    10861 gtctcgttcc gaaatcgtcc atgtcaaccg gcccgaggtc atctggaaca ggctcatcga
    10921 ctgccggctc tgacacggct tcggccggct tggcggatgt ggcgacggcc tctggagcgg
    10981 gtgcaccctc gatgtcgagc tcgaagacac gctctggttc gtcgatgtcc ttcaactgcc
    11041 gttcacccaa cgtgtggacc gagacgccat cgggaagcga gctcccgacc agcgcacgcg
    11101 tcgtctccga cagcagcacc tggccgccgc gcccgagggt gcacaaccgg gccgcccgga
    11161 cgacatcgat gcccaggtac ccctcactgc cgacggcagg ctcgcccgtg tgcaggccca
    11221 gacggacgcg gaccacagaa ccgttcggcc aatcatggga ggcgtgcacc cgctgcgcct
    11281 caacggccgc cgacaccgcg tctcgggcac ggggaaacgc gaagaagaag gcatcgcctt
    11341 gtgtgtcgat ctcgacgccg ccgcgcggcg cgaagctctc gcgcatcagc ttccggtgac
    11401 ccgacaggac ctcggcgtac cgatcgccga gcgccttcag cagcgtggtc gagccctcga
    11461 tgtcgctgaa gacgaaggtg acggttccac tcggcaatcc agacacgcgc ccagtatgcc
    11521 ggtctcgtcg ccggcccggg acaccgtggt cccgtggtcc accaacggtt cccgttctgg
    11581 cagcatcgcc ctcgagttct tcacttcaac ggaggcagca tgagcagcaa agccggggcg
    11641 gccgctggca gcggcgccat ctacggtctc gggatcttcg gcgcactggt ctactactgg
    11701 caggaggccg aacgggcgtg ggagtacgtc tacgcggtct tccaggcgtt cttctggccc
    11761 gcatacatgg tgtactcggc gttctcggcg ctgcacgcgt gacgccggtt cattcttggc
    11821 gcgccaacct cgccccgcag gtcgggaacc acggcgctgc cggcaacgtc ccagcagttg
    11881 actgtgtgag gtgaaggcct gtgcgtatcc ggctgctcgg agtcgccatc gtcaccgcgg
    11941 tgtcgagcgg gtgcggtctg gcgctgagtc cgtcgactga cagctccacc gtcgtggtcg
    12001 aggcccaccc ggttccgcat ccgtatgccg gaccgatgag cgtgcagccg gacacctccg
    12061 acaacgccac ggtgctcgaa cggtccggcg ccgccgggcg tgccctcgag tgcgactacg
    12121 agccgtacga cggcggaggt ggcgactatg acagcggcct ggagtcggtc caggacagcc
    12181 cggcgaaggc gctggagaac ttcctcgagg aagaactgtt cctccagctg ccgaagaccg
    12241 gctaccagct ggaacgggag gacgacggtc gggcgctcct gtcgtacgac gttggacaac
    12301 gcacgaagat cgcgttcatc gctgccgacg gcatccgcga ctacgaggac gacgaaggct
    12361 ggggcatcga gacgtgggcg gagtgcgatc cagcagagtt gccggcaccc gtgacggagg
    12421 cactcggcat cggcgtatgg caggacaggt ccggtcgacg cgtaccagtc gcccagatcc
    12481 gatccttcca aggacccgag cactgcagct ggcaggacgt gacgttcctg gagctcgggc
    12541 ccgagcatca cgcggacgag tacgtgggtc acccgacgcc ggacctcgcc gactggctga
    12601 agacgacgta caccacgaac ccgccatggc caccaggcgt cacggacacc ggcttccgcc
    12661 aggacggccg ggagctgtgg ctcggacccg agcacgatgc ggcctacctc gtcagtcgag
    12721 gtgatccgac gaacgtggaa cgctggccgg cagccaagca acccatcggc tgtgactgag
    12781 tccgggtagg ttgcggggca tgagctccgg caccgagacg acccgtgcgt acgccgaaca
    12841 gctcgaccgc gctgacccgc tggcgtcgta ccgcgacgag ttcgtcatcc ccgacgacga
    12901 cctcgtctac ctcgacggga actcactcgg ccggctgccg aagtcatcgc aggagcgtgt
    12961 caagcacgtg ctcgacgacg actggggcgg ccagctgatc aggtcctggg aagaccgctg
    13021 gatgcacctg ccgacacaga tcggtgacct catcggcacc ggcctgcttg gtgccgcgcc
    13081 cggcgaagtc gtcgtcgggg actcgacgac ggtctcgctg tacaaggcga tgtcggcact
    13141 gctcgacgca cagtctgatc gccgcgccgt gatcatcgag cgagacaact tcccgtccga
    13201 tcgctacctc gtcgagtcgc tggcgaaaca gcgcgacctc gaggtgcgat ggatcgacga
    13261 gaccggctac gagggcgtct cgtccgatca tgtcaagccc ttgctggaca acgacgtggc
    13321 tctcgtctcg ctgagtcacg tggactaccg gtcggccgcc atcgtcgaca tggcgtcgat
    13381 caccgcagcg gtgcacgagg tcggcgcgct gacgttgtgg gacctgtgcc actcggtggg
    13441 gtcgatcccg atcgacctgc atcgtgccgg ggctgatgtc gcggtgggct gcacgtacaa
    13501 gtacctcaac agcgggcccg gcgcgccggc cttcacctac gttcgcaccg acctgcagcc
    13561 tcgactctca cagccgatct gggggtggtg gagccgggcc gagatgttcg acatggcgca
    13621 gggctaccag ccgtacgccg acatgcgttc ctggctggtc gggactccca gcgtgctgtc
    13681 cttggtcgcg atcgagccca gcgtccggtt gatcctggac gccgggatgg acgcgctacg
    13741 ggccaagtcg ctgctcctca cggcatacgc cgtcgacctg ttcgacagtt ggctggcgcc
    13801 gctgggtgcg gggcttgcga gcccgcggga gcccgagcgg cgcggttcgc acgtgacggt
    13861 gacggtgccc gacgcgaagg tgctgtgcgc ccagctgacg cggcgcggtg tgctgccgga
    13921 ttttcgtcgt cctgacggaa tccgcatcgg actggccccg ttgacgacga gattcgtcga
    13981 cgtgtacgac ggcttggcga ctctgcgcgg cctcctggtg tccggagtga cgagatcctc
    14041 cgtcgatgtt gcacaacggg agccttgacg caacaaagca tgatgcggca ggtttcgatc
    14101 ggctctcgtc gtgatccctc gtcacgccgt cccctgccga gcctcccgag gcatcgacac
    14161 gatcgtcgat gcgccgacat ggaggttgtc cgtgaaacgt cttgccacca tcggagcgat
    14221 cgccgcagtc accgcggcag gtctctccgc cgcaccgtcg ggggcggcca tcgcgaccgc
    14281 acccgccgcg agtcgccagc tcgtcacgaa ggcggatccc cacttcacac cgaaggagcg
    14341 cgcggcagtg ctgcgcgagg ccagggccga cgcgcgggac accgcccgta cgctccggct
    14401 accggcgtcg cagtccctgc acccgaccga cgcgctggcc gacaccaacg gtgccgtgca
    14461 catccgctat gaccgtacct accggggtct gcgcgtcatt ggtggagacc tggtcgtcca
    14521 ccagcggccg agtgggaagg tcaagagcgt cgactacgcg tcgcaggcca acgtcgcgct
    14581 gcgcagcatc cacccacgaa ccggcgctcc tgcgaagcgc tcccacatcg tcgtcttcgc
    14641 cgctcgccac cgggccgtcc tcgcctggga gacccacacc atcggcacca ccaggcgcgg
    14701 cggaccgatc tcgcggctga cgtacaccaa cgccaacacc ggtcggcgca tcacgtcgtg
    14761 gagcacggtc gacaccgctg acggcaccgg gaactcgttg tactccggca ccgttccgct
    14821 gaagacggtg ttgtccggct cgacgtacca gctcatcgac cagactcgcg gcagcagcgc
    14881 gatctatgac gccaacaact cgaccggcac cggccgcggc acgctgttca ccgactccga
    14941 caacgtctgg ggcaacggct cgacgacatc caggcaaagc gcagcggtgg acgcggcgta
    15001 cggcgcggcc aacacgtggg acttctacaa gaacaccttc gggcgcaacg gtatccgcaa
    15061 cgacggcgtc gcggcgtaca gccgggtcca ctacggcagg aactacgaga acgcgttctg
    15121 ggacgactcc tgcttctgca tgacgtacgg cgacggcggg tcgacgttca gcccgctggt
    15181 ctcgctcgac gtcgcaggtc acgagatgag ccacggcgtc acgtccaaca ccgccgggct
    15241 ggtctacagc ggtgagagcg gcggcctgaa cgagtccacg tccgacatct tcggcacgat
    15301 ggtcgagttc acggccaaca acgccagcga cccgggtgac tactacatcg gtgaggagat
    15361 cgccagggac ggcacgtacc tgcgccggat ggaccacccg tcgctcgacg gagcctccgc
    15421 cgactgctgg tcgtctggtg tcggcaacct caacgtgcac tactcgtccg gcgtcggcaa
    15481 ccacttcttc tacctgctcg ccgagggctc gggcgccaag acgatcggcg gacgctcgca
    15541 caacagtccc acctgcaacg gctcgtcggt caccgggatc ggacgcgacg ctgccgccag
    15601 gatctggtac cgcgcgttga cgacgtacat gacgtcgacg acgaactaca agggcgcgcg
    15661 cgacgccgcg atcaaggccg cccgcgacct gtacggcgcc acctcgacac agtgcaacac
    15721 ggtggtcagc gccatgaacg ccgtctccgt gccggtgggt gccgagacgt gctccggctc
    15781 gaatccgcca ccaccgggcg gcaacctgtt gctcaacccg ggcttcgagt ccggcaacgt
    15841 gaactggtcg gcgaccagcg gtgtcatcgg gaccggcagt ggctaccagc cgcgcaccgg
    15901 tgcctacaac gcctggctcg acggctacgg gacgacgcac accgacacgc tgtcgcagac
    15961 catcacgatt ccgtccggca agtcgacagc atcgctcggg ttctacctgt ggatcacgac
    16021 ccaggaacgc ggctcgacgg tctacgacac gttgcgtgtg caggccatca ggaacggcgt
    16081 cacgacgacg ctggcgacgt actccaacgc gaatgccggc agcgggtatg tgctgcggac
    16141 gctgagtctc aacagctaca tcggccagac cgtcacgctg aggttcaccg gcaccgagga
    16201 ctcctcgctc gccacgagct tccgcatcga cgacacctcg gtgacaacca cgtagccgcc
    16261 gtcggtggtc gagcccttcg acaagctcag gaaccacttg tcgagaccgc ggcccttggt
    16321 ttcgacaagc tcaaccagcg gacgaaaagc gccgcaccca cgagcagggg cgccacccgg
    16381 acctgggtgg cgccccttgt ttcgcatacg ctcggggaca tgaccccgcg ccccggtccg
    16441 gacgtcgacg cacccgcaga tccggagcac gatccgcgcg ccgcgtacgc ggtcgatcca
    16501 ggcctcgtac gcaggttggc aactcggatc gatgccggtt cgggccggac tgagacgacg
    16561 tacgcgccgt tcaccggtca gccgatcgcg acgctcaccg tgtcgacacc ggacgacgtg
    16621 gctgctgcgg tccagcaggc ccgccaggct cagcgcgcgt gggcgcgagt cccggtggag
    16681 ctgcggcagc ggatcttcct gcggttccac gacctggtgc tcgaccggca agacgagctc
    16741 ctcgacctga tccagtggga gtcggggaag gcgcgcagcc acgcgttcga ggaggtggcg
    16801 cacgtcgcga tgaccgcgcg ctactacgcc cggaccggcg gcaagcagct ggccggcggc
    16861 aggcggtacg gcatcctgcc gctcttgacc cgcgtggacg tccaccaggt tcccttgggt
    16921 gtcgtgggcg tgatcggtcc gtggaactac ccgctgacga tggccatggc tgacgggctg
    16981 gccgcgctgt acgccggtaa cgcggtcgtg ctgaagccgg acgcgcagac gccgctcagc
    17041 gcgctcctcg gggtcgacct gctgtccgaa gccggtgtgc cgccggacgc atggcgtgtc
    17101 gtctacggcg acgggccggt gatcggtggc gcgatcctcg accacgtcga ctacgtcagc
    17161 ttcaccggct cgaccagcat cggccggtcg atcgccaccc gcgcggccgc acggttgatc
    17221 ggcgcctcgc tggagctcgg cggcaagaac ccgatcctcg tcctgcgcga cgccgacctg
    17281 gagcgggcgg ccgaaggagc ggtccgcgcg tgcttctcgt cggcgggtca gctctgtgtg
    17341 tcgatggagc ggatgtacgt cgccgaccag atctacgaca ggttcctcga ccgtttcgtc
    17401 cggcgtaccg aggcaatgcg gctctcgccg gctctcgact actcggcgga catgggatcg
    17461 ctcgtctcgc agcgacagct cgacacggtg accgcgcatg tccaggacgc cgtgcgacaa
    17521 ggggcgatgg tcgtgaccgg cggccacgcc aggccggacg tcgggccgct cttccacgag
    17581 ccgaccgtgc tcgccggcgt gacgccgacc atgcgctgct tcgccgagga gacgttcggg
    17641 ccggtggtct cggtctaccg gttctccgac gagcaggaag ccgtcgcgcg cgccaacgac
    17701 ggggtcttcg ggctcaacgc cagcatctac acccgggacg ccgcgcgcgg tcgtcggctg
    17761 gcacggcagc tgcggtgcgg ctcggtgaac gtcaacgagg ggtacgccgc cagcttcggt
    17821 tcgctcgact ccccgatggg cggcatgcgc gactcgggtc ttggccgccg gcaagggccg
    17881 gagggcctgc ttcgctacac cgagacgcag accgtcgcga cccagcgggg gctgccgttc
    17941 cgaccggtcc tcggcatgtc acagcagcgg tacgcgaaga ccttcacatc gacgctgcgg
    18001 ctgctgaagg agctgcgccg tccgtgaccc acgactacga cgtgatcgtc gtcgggtcgg
    18061 gtttcggtgg gtcggtcgcg gcgcttcgac tgacggagaa ggggtatcgc gtcggtgtgc
    18121 tcgaggccgg tgcgaggttc gacgagacgt cgtaccccgc cacgtcgtgg gacctgcgga
    18181 ggttcctgtt cgcccccgcc ctcggttgct acggcatcca gcgcatcgac gtgctgaaga
    18241 actgcgtgat cctgtccgga gccggtgtcg gcggcggttc cctggtgtac gccaacacgc
    18301 tgtacgagcc gctcgacgcc ttctaccgtg acccgtcctg gagccacatc accgactggc
    18361 ggaccgagct cgcgccgtac ttcgaccaag ccaggcggat gctcggcgtg acgacgaacc
    18421 cgacgatgac gcccagcgac gttgtcatga agcaggttgc cgacgagctc ggagtcggcg
    18481 cgacgttccg ccgtacgccg gtgggcgtgc tcttcgggga gccaggtgct gacgtgcccg
    18541 acccgtactt cggtggtgcc ggccctgacc gcaaggcgtg catcgagtgc ggggagtgca
    18601 tgaccggttg ccgacatcac gccaagaaca cgctgacgaa gaactacctc catctcgccg
    18661 agcaggccgg tgccgtcgtc cacccgctca ccaccgtcac cgggatacgc ccgctggcgg
    18721 ttggcggtta ccgggtcgcc acgcgtccga cagcgttgca tggacgacgg gcccggcggg
    18781 cgagtcgagc gttcacggcc gagcaggtcg tgctggctgc gtcgacgctc gggacgcagc
    18841 ggctgctgca ccggatgcgc gacgagggtc tgctgcccag gctctcgcca cgtctcggtc
    18901 acctgaccag gacgaactct gagtcgatcc tcggagctgt cgcttcgggc ggcgacgtcg
    18961 acttcagccg cggggtcgcg atcacgtcgt cgttccaccc cgacgatcac acccacatcg
    19021 agccggtgcg gtacggcaag ggcagcaatg cgatgagcct gctgcagacc gttctgaccg
    19081 acgacgacgg cggcgttgcc cgttggcgcg tctggctgcg cgagatgtgg aggcagcgac
    19141 ggtcgctggc aagcctgtac gacgtccggc actggtcgga gcggacggtc atcgccctgg
    19201 tcatgcagac gcacgacaac tcgatcacga cgtacacgaa gcgctccggt ctcacgcgac
    19261 gtcgccggct gacgtcgcgg catggccacg gagcgccgaa cccgacctgg attcccgtcg
    19321 gcaacgacgc cgtacgacgg
//
LOCUS       DATBFZ010000508         1510 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_4503788, whole genome shotgun sequence.
ACCESSION   DATBFZ010000508 DATBFZ010000000
VERSION     DATBFZ010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4503788"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..798
                     /locus_tag="VH419_15695"
     CDS             <1..798
                     /locus_tag="VH419_15695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7045114.1"
                     /translation="KVGSAAAVTVNSASTTFSVANTGQTAVTYYATDRAGNVETTKSY
                     TVRRDNLAPTTTASSNPAPNENGWVNSTPTLTLAATDSTSAVASITYKIGSAAPVTVN
                     AATASFPVAATGTTAVTYYATDLAGNVESTKTYTVRRDSTAPTASVTFPVNNGSYTNK
                     AFEDGCGTSAKDVCGNVSDNVGVDTVIVGIYSNDRAACLYRTGAWGDCFESNGDPDPL
                     LPVATVTGTTWRYVTGNLPKGSYIAAVGAYDLAGNSVISATLAFTTQ"
     gene            938..>1510
                     /gene="pdxT"
                     /locus_tag="VH419_15700"
     CDS             938..>1510
                     /gene="pdxT"
                     /locus_tag="VH419_15700"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009079325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HEY7045115.1"
                     /translation="MPPTSGSPTIGVLALQGDVREHLRMLTDAGVSAVPVRRPAELAA
                     VDAMVLPGGESTTMHKLATIFELFEPLRDRIGSGMPALGTCAGMILLADRIEGGIAGQ
                     QTLGGLDVVVRRNAFGRQVDSFEVDVDFAPFDCPFHALFIRAPWVEKVGDDVDVLATV
                     ASGEAAGRIVAVRQGVVMATSFHPEITQDDR"
BASE COUNT          257 a          532 c          498 g          223 t
ORIGIN      
        1 aaggtcggct cggcggcagc ggtcacggtg aacagtgcct cgaccacctt ctcggtcgcc
       61 aacaccggac agacggcggt gacgtactac gccacggatc gtgccggaaa cgtcgagacg
      121 accaagtcgt acaccgtcag gcgagacaac ctcgctccga cgaccacggc gtcgagcaac
      181 cccgccccga acgagaacgg ctgggtcaac tcgaccccga cgctgacgct cgccgccacc
      241 gactccacct ctgcggtcgc gtccatcacc tacaagatcg gctccgccgc accggtcacg
      301 gtcaatgcgg caaccgccag ctttccggtc gccgccacag ggacgacggc cgtgacgtac
      361 tacgcgacgg acctcgctgg gaacgtcgag tcaacgaaga cctacaccgt gaggcgggac
      421 agcacagctc cgaccgcgag cgtcacgttc ccggtgaaca acggctcgta cacgaacaag
      481 gcgttcgagg acggatgcgg cacgtcggcc aaggacgtct gcggcaacgt ctccgacaac
      541 gtcggtgtcg acacggtgat cgtcggcatc tactccaacg atcgggcggc gtgcctgtac
      601 cgcaccgggg cctggggcga ctgcttcgag agcaacggcg acccggaccc gctgctcccc
      661 gtggcgacgg tgacgggcac gacctggcgt tacgtgaccg gcaacctccc caagggctct
      721 tacatcgccg cggtgggcgc ctacgacctg gccgggaact cggtgatctc cgcgaccctg
      781 gccttcacga ctcaatgaac gacgagctgc acgtggccga tcacccggca acgaccacgt
      841 gaccaccgac cgccggccgg ttcccgagct gtgacggggg ccggccggcc tccacctctg
      901 ggatcacagc gcacgggggg ccacctacga tgtgggtgtg ccccccacct ccggatcacc
      961 gacgatcggc gtgctcgcgc ttcagggtga cgtgcgggag cacctgcgga tgctgaccga
     1021 tgccggcgtg tccgcagttc ccgtacgccg acccgcggag ctggctgccg ttgacgccat
     1081 ggtgctgccc ggcggtgagt cgaccacgat gcacaagctc gcgacgatct tcgagctgtt
     1141 cgagccgctg cgggaccgga tcggatcggg gatgcctgct ctcgggacgt gcgcaggcat
     1201 gatcttgctg gccgaccgga tcgaaggcgg gatcgccggc cagcagacgc tcggcggtct
     1261 cgacgtggtc gtccggcgca acgcgttcgg ccgccaggtg gactccttcg aggtggacgt
     1321 cgacttcgca ccgttcgact gcccgttcca tgccttgttc atccgggccc cctgggtcga
     1381 gaaggtcggc gacgacgtgg acgtgctcgc gaccgtcgcg tcgggagagg ccgccggtag
     1441 gatcgtcgcg gtgcgccagg gcgtcgtcat ggccacgtcg ttccatcccg agatcaccca
     1501 ggacgaccgg
//
LOCUS       DATBFZ010000509         1995 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_5487194, whole genome shotgun sequence.
ACCESSION   DATBFZ010000509 DATBFZ010000000
VERSION     DATBFZ010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 1995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1995
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5487194"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1143)
                     /locus_tag="VH419_15705"
     CDS             complement(<1..1143)
                     /locus_tag="VH419_15705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007078796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY7045116.1"
                     /translation="MPTRSDLRNIAIVAHVDHGKTTLVDAMLKQSGGWHARTEMTERV
                     MDSMDLEREKGITILAKNTSVTYHPTVGDPVTINIIDTPGHADFGGEVERGLTMADGV
                     VLLVDASEGPLPQTRFVLRKALAARMPIILVVNKVDRPDARIKEVVDDTYELFLDLDA
                     DEAQIDFPIVYASARDGVASLTQPADGTVPTDSTSLEPLFRTILTTMPAPEFVDGAPL
                     QAHVTNLDASPFLGRLALCRVREGTIRKGQAVAWCKSDGTIERVRISELLMTDGLERK
                     SAEQAGPGDIIAVAGIGDIMIGETLAEADDPVPLPLITVDEPAISMTIGTNTSPLAGR
                     VKGSKVTARLVKDRLDRELVGNVSLRVLPTERPDTWEVQGRGELALA"
     gene            1554..>1995
                     /locus_tag="VH419_15710"
     CDS             1554..>1995
                     /locus_tag="VH419_15710"
                     /inference="COORDINATES: protein motif:HMM:NF013358.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HEY7045117.1"
                     /translation="MAEPEAPRVAAAGGIADPAPLGLAAFALTTFLLSASNAGWTRGT
                     GAAWLGYALAYGGLIQLLAGMWEFRRNNTFGAVAFSSYGGFWIGLFLYQVFVKSSNPT
                     NDLGWILLAFAIFNLYMLIAATRINAAVTAVFLTLQLTEIVLFIG"
BASE COUNT          319 a          659 c          644 g          373 t
ORIGIN      
        1 cgcgagcgcg agttcgccgc gaccctgaac ctcccaggtg tccggccgct cggtgggcag
       61 cacgcgtagc gatacgttgc cgaccagctc acgatctaga cggtccttca ccagccgcgc
      121 ggtgaccttc gaccccttga cacgcccggc gagcggtgag gtgttcgtgc cgatcgtcat
      181 ggagatcgcg ggttcgtcga cggtgatgag tggcagcggt accggatcgt ccgcctccgc
      241 cagggtctcg ccgatcatga tgtcgccgat gccggcgacc gcgatgatgt cgcccggtcc
      301 ggcctgttcg gccgacttac gctcgaggcc gtcggtcatc agcaactcgg agatgcgtac
      361 ccgctcgatg gtgccgtccg acttgcacca cgcgaccgcc tgtcccttgc ggatcgtccc
      421 ttcccgcacg cggcacagtg cgaggcgacc gaggaacggc gacgcgtcga ggttcgtgac
      481 atgcgcctgc agcggcgcgc cgtccacgaa ctccggcgcc ggcatggtgg tgaggatcgt
      541 gcggaacagc ggttcgagcg acgtcgagtc ggtcggcacg gtgccgtccg ccggctgggt
      601 cagcgacgcg acgccgtccc gggcggacgc gtagacgatc ggaaaatcga tctgcgcttc
      661 atccgcgtcg aggtcgagga acagctcgta agtgtcgtcg acgacctcct tgatccgcgc
      721 gtccggccgg tccaccttgt tgacgacgag gatgatcggc atgcgcgcgg cgagtgcctt
      781 gcgcagcacg aagcgggtct gcggcagcgg gccctcggat gcgtcgacga gcagcacgac
      841 tccgtccgcc atcgtgaggc cgcgctcgac ctcgccaccg aagtcggcgt gcccgggcgt
      901 gtcgatgatg ttgatcgtga ccgggtcacc gaccgtcggg tggtacgtga ccgatgtgtt
      961 cttcgcgaga atcgtgatgc ccttctcgcg ctcaagatcc atcgagtcca tcacgcgctc
     1021 ggtcatctcg gtgcgtgcat gccaaccacc ggactgcttc agcatggcat cgacgagcgt
     1081 cgtcttgccg tggtcgacgt gggcgacgat cgcgatgttg cggaggtcgg aacgcgtagg
     1141 cataggtacc agtgtcccgg ttgccgagcc accctgtctg ccgaccccga cggtgggcaa
     1201 tttcgatcac actgcgtgtg acgaaacggc gatgcaatcg acggtcatcg ggtgtgcgac
     1261 ggcgtcaaac gacacgccaa tacttagcta ttactggata ttgcggcttg acgatcaacg
     1321 cgctactttc tcgataatca acgtttcgtg aggtagcgat gcacctggta gccaaggttg
     1381 tggtcgccaa ttcgccactc acgagggacc cggtccctcg cgggccccat cgttgtttcc
     1441 acccttcctt cgccacaggt ctcttgctgc cgacaggttc gcgagcgacg actcgcgcct
     1501 gccaggcgac cggggacagg gcgtccacgc tacggtagag agaggccacc accatggctg
     1561 aaccggaagc acctagagtc gcggcagccg gcggcattgc cgatcccgca cccctgggac
     1621 tcgcggcctt cgcgctgacg acgttcctcc tgtccgcgtc caacgcgggc tggacccgag
     1681 gcaccggagc cgcgtggctc ggctacgcgc tcgcgtacgg cggcctcatc caactcctgg
     1741 ccggcatgtg ggaattccgc cggaacaaca cgttcggcgc cgtcgcgttc tcgtcgtacg
     1801 gcgggttctg gatcggattg ttcctctacc aggtgttcgt caagtcgagc aacccgacga
     1861 acgatctggg ctggatcctg ctggccttcg ccatcttcaa cctctacatg ttgattgcgg
     1921 cgacgcgcat caacgcggca gtcacggccg tgttcctgac cctgcagctg accgagatcg
     1981 tgctgttcat cggct
//
LOCUS       DATBFZ010000510         2031 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_5487468, whole genome shotgun sequence.
ACCESSION   DATBFZ010000510 DATBFZ010000000
VERSION     DATBFZ010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5487468"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            60..746
                     /gene="phoU"
                     /locus_tag="VH419_15715"
     CDS             60..746
                     /gene="phoU"
                     /locus_tag="VH419_15715"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEY7045118.1"
                     /translation="MESRPLRVTFHEELAELEDTLQREGNLIVRSVQAATRAMVERDL
                     ELADEVISFDDDVDSAYLAIQEGVQSLLARQTPVASDLRLVLAMLHVNLHLERMADYC
                     VTVAKLTKLSGNAPSDPRIVAALAEMGVRAEEMTRVAMRSFQRRDLERAEALVDLDEL
                     IDRTNRRLAGHVLELDAEHREWGLRMLVVSRCLERIGDHAVDIGEQTAYLVTGEFREF
                     TDASHPEEIG"
     gene            861..>2031
                     /locus_tag="VH419_15720"
     CDS             861..>2031
                     /locus_tag="VH419_15720"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF012470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HEY7045119.1"
                     /translation="MAGFRRPAVGAVVLVAAGLVAAGLAVAGTAGKGDEVRSGGKAQN
                     VVYFLGDGMGTQEITAARYYQYGAAGKMNIDRLLFTGFKTTWSIKPAAAPPYLPDYDP
                     DSASTGTMWATGKKTLDERISQGPSSAITVPGQNFRTVLEIAQKDGKRTGNVSTAEIT
                     DATPAVLDSHISQRACQSPANAQATCPTETKAAGGLGSIAEQTVDHHIDVVLGGGRAR
                     FDAGGGLIAGGPDDGQTVEQSAARQHIKEINTAAQLAALEPSDTPVLGLFAAGNMTTE
                     WTGPVATLGDGNAAHTCVTTNRPANEPSLADMTRKALDLLSSDKPGRGGGKDRGFFLQ
                     VEGASIDKQDHAANACAQIGETVAFDTAIGVALQFQRTHPDTLIVVTADHSHTSQI"
BASE COUNT          353 a          704 c          688 g          286 t
ORIGIN      
        1 ggggtgctca ccgtgatcct cctgttctcg acgttctgag gcgggctagc atcgaacccg
       61 tggagagccg tccgctgcgc gtcacgttcc acgaggagct cgccgagctg gaggacacgc
      121 tgcagcggga gggcaacctg atcgtccggt cggtgcaggc tgcgacccgg gcgatggtcg
      181 agcgggacct cgagctggcc gacgaggtca tctccttcga cgacgacgtc gacagcgcct
      241 acctcgcgat ccaggagggc gtgcagtcgc tcctcgcccg ccagactccc gtcgcctcgg
      301 acctccggct ggtgctggcg atgctgcacg tgaacctgca tctggagcgg atggccgact
      361 actgcgtcac cgtcgcgaag ctgacgaagc tgtccggcaa tgcaccgtcg gacccgcgca
      421 tcgtcgccgc gctggcggag atgggcgttc gcgccgagga gatgacccgg gtggccatgc
      481 gctcgttcca gcgacgcgat ctggagcgcg ccgaggcgct ggtcgacctg gacgagctga
      541 tcgaccggac caaccgccgc ctcgccggcc acgtgctcga gctggacgcc gaacaccgtg
      601 aatggggcct gcggatgctg gtcgtgtcgc gctgcctcga gcggatcggc gaccacgccg
      661 tcgacatcgg cgagcagacc gcctacctcg tcaccggcga gttccgcgag ttcacggacg
      721 cctcacatcc ggaagagatt ggttagcggg gccgtcccaa cgttcaccag ctgatcgcct
      781 ctcgttaacc catcctgccg gcgcccgcgc ctagcctcgg ctccgacagg caggcggact
      841 atcgacgaaa ggggaggcga atggctgggt tcaggcggcc ggcagttggc gctgtggtgc
      901 tggttgcggc cgggctcgtc gcggccgggc tggccgttgc cggcacggcc ggcaagggcg
      961 acgaggtgag gagcggcggc aaggcgcaga acgtcgtcta tttcctcggc gacgggatgg
     1021 gaacgcagga gatcacagcg gcgcggtact accagtacgg cgctgccggc aagatgaaca
     1081 tcgaccggct gctcttcacc gggttcaaga ccacctggtc gatcaaaccg gcagctgccc
     1141 cgccgtacct gcccgactac gaccccgatt cggcatcgac cgggacgatg tgggcaaccg
     1201 gcaagaagac cctcgacgag cgtatctctc agggcccgag tagtgccatc accgttcccg
     1261 ggcagaactt caggaccgtg ctcgagatcg cgcagaagga cggcaagcgc accggcaatg
     1321 tgtcgaccgc ggagatcacg gacgcgacac cggccgtgct cgactcgcac atctcgcagc
     1381 gcgcctgcca gtcaccggcc aatgcccagg cgacctgccc gaccgagacg aaagctgcgg
     1441 gcgggctcgg ctcgatcgcc gagcagaccg tcgaccacca catcgacgtc gtcctcggcg
     1501 gtggccgtgc tcgcttcgac gccggcggcg gcctgatcgc cggcgggccc gacgacggcc
     1561 agaccgtcga gcagtcggcc gcacggcagc acatcaagga gatcaacacc gctgcgcagc
     1621 tcgcagcgct cgagcccagc gacacgccgg tcctcggcct gttcgctgcc ggcaacatga
     1681 ccaccgagtg gaccgggccg gtggcgacgc tcggtgacgg aaacgcggcc cacacgtgcg
     1741 tgaccacgaa ccggcctgcg aacgagccga gcctggccga catgacgcgc aaggcactcg
     1801 acctcctgtc ctccgacaag cccggcaggg gcggcggaaa ggatcgtggc ttcttcctcc
     1861 aggtcgaagg cgcatcgatc gacaagcagg accacgccgc gaacgcctgc gcgcagatcg
     1921 gcgagacggt tgcgttcgac accgcaatcg gtgtcgcgct gcagttccag aggacgcacc
     1981 cggacacgct gatcgtcgtc acggccgatc actcgcacac cagccagatc g
//
LOCUS       DATBFZ010000511         1799 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_4505810, whole genome shotgun sequence.
ACCESSION   DATBFZ010000511 DATBFZ010000000
VERSION     DATBFZ010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4505810"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="VH419_15725"
     CDS             <1..213
                     /locus_tag="VH419_15725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7045120.1"
                     /translation="DVTDAELLDAALDGLAGYAGQLNRTRSERDALESALRGSSEAAT
                     AGSGRTRTLRTKASAVLRGLRRRVRS"
     gene            316..>1799
                     /gene="acnA"
                     /locus_tag="VH419_15730"
     CDS             316..>1799
                     /gene="acnA"
                     /locus_tag="VH419_15730"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HEY7045121.1"
                     /translation="MNSFDALADLEVGGTSYAIYRLDAVTGEGLDVTSLPYSLKVLLE
                     NLLRTEDGTNITADDVRALAGWDPEAEPSKEIQYTPARVIMQDFTGVPAVVDLATMRE
                     AMQDLSGDPAKINPLAPADLVIDHSVIADEFGTPQAFQRNVEFEYERNRERYQFLRWG
                     QGAFADFKVVPPGTGIVHQVNIEHLAQVVFTREVSTGSTTDVLAYPDTCVGTDSHTTM
                     VNGLGVVGWGVGGIEAEAAMLGQPVSMLIPRVVGFKLSGELPDGATATDLVLTITEML
                     RQHGVVGKFVEFYGSGVAAVPLANRATIGNMSPEYGSTIAIFPVDDETLRYLRFTGRS
                     DEQVALVEAYAKEQGLWHDPGREPRYSEYLELDLSTVVPSIAGPKRPQDRIAVSDAKD
                     GFRAALAEYVEAEADEEEKGYDEAAAESFPASDPPSYHGDNDSEPPHGKPSAAAGSEG
                     RPSRPVLVKLEDGSEFEIDHGAVVIAAITSCTNTSNPSVMIGAAL"
BASE COUNT          315 a          584 c          633 g          267 t
ORIGIN      
        1 gacgtgaccg atgccgagct actcgacgca gcgctggacg gactcgcggg gtatgcgggg
       61 cagctgaaca ggacccgttc cgaacgcgac gccttggagt cggcgctccg aggttcgagc
      121 gaggcggcca cggctggttc ggggaggacg cgaacgctgc gtacgaaggc gagtgccgtc
      181 ctccgtgggt tacgacgccg tgtccggagc tgacgtaccg gagatgcatc acggcggacg
      241 cgttgctatg ctgatatctt gacgtcaaga tacctgggcg agagaggcga agacgtgggc
      301 acccaaggca gttcggtgaa cagtttcgac gcgttggcgg acctcgaggt cggtggcacg
      361 tcgtacgcga tctatcggct cgatgccgtg acaggggaag ggctcgacgt cacgagcctg
      421 ccgtacagcc tcaaggtgct gttggagaac ctgctgcgca ccgaggacgg caccaacatc
      481 actgcggacg acgtcagagc gctggccggc tgggacccgg aagccgagcc gagcaaggag
      541 atccaataca cgccggcccg ggtgatcatg caggacttca ccggagtacc ggccgtggtc
      601 gacctcgcca cgatgcggga ggcgatgcag gatctctccg gggacccggc caagatcaac
      661 ccgctcgcgc cggcggacct ggtgatcgac cattccgtga tcgccgacga gttcggtacg
      721 ccgcaggcat tccagcgcaa cgtcgagttc gagtacgagc gcaaccgcga gcgctaccag
      781 ttcctgcggt gggggcaggg agccttcgcc gacttcaagg tcgtcccacc cggcaccggc
      841 atcgtgcacc aggtgaacat cgagcacctc gcacaggttg tcttcacgcg tgaggtttcg
      901 acaggctcaa ccaccgacgt gctggcctac ccggacacgt gcgtgggcac cgactcgcac
      961 acgacgatgg tcaacgggct cggcgtcgtc ggctggggcg tcggcggcat cgaggccgag
     1021 gcggcgatgc tcggccagcc tgtctcgatg ctgatcccgc gggtcgtcgg gttcaagctg
     1081 tccggcgagc tgcccgacgg cgcaaccgcg accgacctgg tgctcacgat caccgagatg
     1141 ctccggcagc acggggtggt cgggaagttc gtcgagttct acggctccgg tgtcgccgcc
     1201 gtcccgcttg ccaaccgagc caccatcggc aacatgtccc cggagtacgg ctcgacgatc
     1261 gccatcttcc cggtcgacga cgagacgctt cgctacctgc ggttcaccgg gcgcagcgac
     1321 gagcaggtcg cgctcgtcga ggcgtacgcg aaggagcagg gactgtggca cgacccgggc
     1381 cgtgagccgc gctactccga gtacctcgag ctcgacttgt ccacggtcgt tccgtccatc
     1441 gcagggccga agcggcctca ggaccggatc gcggtcagcg acgccaagga cggcttccgg
     1501 gcggcgctcg ccgagtacgt cgaggcggag gcggacgagg aggagaaggg gtacgacgag
     1561 gcggccgccg agtcgttccc cgccagcgac ccgccgtcct accacggtga caacgactcc
     1621 gagccgccgc acgggaagcc gtccgcggcc gctggctcag aagggcggcc gagccggccg
     1681 gtcctggtga agctcgagga cggcagtgag ttcgagatcg accacggtgc ggtcgtgatc
     1741 gcggcgatca cgtcctgcac gaacacgtcg aacccgtcgg tgatgatcgg cgccgccct
//
LOCUS       DATBFZ010000512         9456 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_5165686, whole genome shotgun sequence.
ACCESSION   DATBFZ010000512 DATBFZ010000000
VERSION     DATBFZ010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 9456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9456
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5165686"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /gene="tatB"
                     /locus_tag="VH419_15735"
     CDS             complement(<1..188)
                     /gene="tatB"
                     /locus_tag="VH419_15735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020385678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HEY7045122.1"
                     /translation="MFDIGLPEFLVLAVVALFVFGPDKLPDVARQAGRMLRSARQMLT
                     QAKSQLSDELGPEFADLDL"
     gene            complement(260..1408)
                     /locus_tag="VH419_15740"
     CDS             complement(260..1408)
                     /locus_tag="VH419_15740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017934850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HEY7045123.1"
                     /translation="MTSPLLERVQAALATVNDPEIRRPITELGMVEDVTVGDDGQVAV
                     TVLLTVSGCPMRETLTRDVTAAVSNVSGVSTVDVRLGVMSDEQRQQLQQDLRGGQPAK
                     EIPFTRPDSLTKVLAVASGKGGVGKSSITVNLAMAMVRSGRKVGILDADIYGHSVPAM
                     LGVADERPTQVEDMIMPVPALGLSVISIGMLKPRRDQVVAWRGPMLDRALVQMLTDVY
                     WGDLDVLLLDLPPGTGDMAISIGQHLPSSEVLVVTTPQAAASEVAERAGTMASMMHQR
                     VTGVIENMSYLVCPHCGPDHRIDVFGSGGGAQVAATLSKRFGYDVPLLGEVPLDTRLR
                     AGGDAGEPIVLAAPDTPPAQVLTDIATVLTAKGRGLAGMQLGLTPAGR"
     gene            complement(1405..1983)
                     /locus_tag="VH419_15745"
     CDS             complement(1405..1983)
                     /locus_tag="VH419_15745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020385689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HEY7045124.1"
                     /translation="MAREDVRDEERQASRRTTSRTARLDVPRETRRQLVRRPTYDPER
                     FGRFAESFARFMGTAQFIAYMTVFVILWITINLVGVWGLEWDPYPFILLNLIFSTQAS
                     YAAPLILLAQNRQEARDRVVAEQDREQDVRAHADMEFLAREMASLRMAVGEVATRDFV
                     RSELRSLLSDLDDRAQDDRDAEPVAETRSLDA"
     gene            complement(1973..3262)
                     /locus_tag="VH419_15750"
     CDS             complement(1973..3262)
                     /locus_tag="VH419_15750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017934852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEY7045125.1"
                     /translation="MSVAPTRVYIARLAGLLVFDPNGDQVGKVRDVVAAVRAENVQPR
                     VLGLVVEVLGRRRIFVPMTRVTSVDSGQVITTGLVNMRRFEQRAGETLVLGELLDRKV
                     TITETGVEGTVYDIAMEQARTRDWVISRVAVQQGAKRFGRRGQTHVFEWSEVAGLAES
                     EAGQGATHMLAAIDNMRAADLANMIHDLSPKRRGEVAAALDDERLADVLEELDEHDQV
                     QILSQLENERAADILEEMSPDDAADLIAELPPDTAEALLALMEPDEAEDVRRLLTYEE
                     HTAGGLMTTDPVILPPDATVAEALARIRHSELTPSLAAMVYVCRAPLETPSGRFLGVG
                     HFQRLLRESPSGLVSGVLDSDLEALRADAPLEEVARHLAYYNLVASPVVDEDGHLLGV
                     VSVDDVLDHLLPENWRQRADDSTVTAAASPEGASRGA"
     gene            3438..4457
                     /locus_tag="VH419_15755"
     CDS             3438..4457
                     /locus_tag="VH419_15755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020574710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEY7045126.1"
                     /translation="MNAPSTHTPSERAATNAETTDLMRPVEGRLRFEVVDVFTDRPFA
                     GNPLAVVYGAEGLATEQLQAIALEFNLSETTFPVVASDEDRAAGADYRVRIFTPTEEI
                     PFAGHPTLGTAWSLRQAGLIDPGARGQRCGAGLIGVDVPEDPAGNVELTAAPRDEPRA
                     LSAADVATVAALVGLTADDVTGSAYTAGCGLSWIFLPVTREAVARARPGSQPISEAGL
                     DLGALVDPLDGVDVYAVRDVNGNDDVNGNDGVSVESRVFVPGFGIPEDPATGSAAAGL
                     GLVLAASGVAAAVGTTAYTISQGVQMGRPSTVHGRVEVTGGRATRVHVAGSVVPVSTG
                     TIAIP"
     gene            complement(4513..5322)
                     /locus_tag="VH419_15760"
     CDS             complement(4513..5322)
                     /locus_tag="VH419_15760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7045127.1"
                     /translation="MGDAREPDDEAPRAFIDQVFEDQSRPLVEDDGVGADQEEWSDEA
                     AASEVDEAAQDGRHPASPDPAADLDAVEEVEPAEEVEPAEEVAPPDPEPTVSLDPADE
                     ALVDEAMKKSGLIWVTTPDVPAGRAFWHAWVDGTAYVLTGSGEQPDPGWEEHAPVSVL
                     VRSKDTVHRLVTFDAYASRVLPEADDWEAATAALATGRLNLRDAESAPQRWAAGAAVI
                     YRLRPAGTISEKPGSYPSESHRAAPVPTAATTAGKPPWVLHKRGHRGRPLS"
     gene            complement(5315..5530)
                     /locus_tag="VH419_15765"
     CDS             complement(5315..5530)
                     /locus_tag="VH419_15765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7045128.1"
                     /translation="MIGPDIETPTTPTTINATVLAEPPTAVRGPAAGDAADAVDRVGG
                     IDGVGGVDGGPVTLPSHHRLWHASTRG"
     gene            5529..6356
                     /locus_tag="VH419_15770"
     CDS             5529..6356
                     /locus_tag="VH419_15770"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY7045129.1"
                     /translation="MAAATAVPALAMSFWRNGLGALAVLPFALVSGRAELAALSRRQL
                     RLGLLAGAMLAAHFGTWVSSLRLTSVASATALVSLQVAWVVVLTRLLGQPVVRRVWLG
                     LVLALSGVIVISGVDFSVSTEALTGDALAFAGAFFAAVYTMVGGRVREELSTTTYTFV
                     CYSTSALLLLACCVVGRLQVVGYDAKSWLLIVGVTVSAQLLGHTVFNHLLATISPTIV
                     SMALLLEVPGAALLAAVFLSQAPPTAVYAGLALIVAGLAIVVGARSVSSAPREAPVD"
     gene            6453..7412
                     /locus_tag="VH419_15775"
     CDS             6453..7412
                     /locus_tag="VH419_15775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020385694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HEY7045130.1"
                     /translation="MPEAPRPRRSCLAVPGSNPRFLEKAKGLDADQVFLDLEDSVAPI
                     AKADARGSVIEALNSGGWGEKVRVVRVNDWTTEWTYRDVTQVVEAAGANLDCVMLPKV
                     QSADQVVALDLLLTQIEKTVGLELGRIGIEAQIENARGLVNVDAIVTASPRVETVIFG
                     PADFMASINMKSLVVGEQPPGYDVGDAYHYILMRILMAARTHDKQAIDGPYLQIRDVD
                     GFKRVAARSAALGFDGKWVLHPDQIAAANEVYSPLQSDYDHAENILDAYAHSTSESGG
                     ATGAVMLGDEMIDEASRKMALVVSAKGRAAGMTRTDVWTPPND"
     gene            7511..8713
                     /locus_tag="VH419_15780"
     CDS             7511..8713
                     /locus_tag="VH419_15780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020385697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY7045131.1"
                     /translation="MGRLCETDGLTDVQTEILKTVREFVGKEILPVANQLEHADEYPE
                     KIVEGMKDLGLFGLTIPEEYGGLGESLLTYALVVEEIARGWMSVSGIVNTHFIVAYML
                     MQHGTDEQKRRYLPQLATGDVRGSFSMSEPGCGSDVSAIRTRAVRDGDGFVVTGEKMW
                     ITNGSTSNLLAVLVKTDEGPEYAGKPHRNMTTFLVEKDPGIGAQAAGTLTIPKKIDKM
                     GYKGVDTTGAVFDGYRIPSDQVLGGQTGRGFYHMMDGVEVGRVNVAARGCGVAWRAFE
                     LGVAYAQQREAFGRKIAEHQAVLFRLAEMATKVEAAHQMMVRAARKKDSGERNDLEAG
                     MAKYLASEYCAQVVEDSFRIHGGYGYSTEYEIERLYREAPMLLIGEGTADIQRMIIGR
                     RLLEDYKL"
     gene            complement(8769..>9456)
                     /locus_tag="VH419_15785"
     CDS             complement(8769..>9456)
                     /locus_tag="VH419_15785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7045132.1"
                     /translation="RGWVAALACVAVVSACSSASDTEQSGNDGNDGNDTVSSATQTEP
                     DSSTPPTTTEPSESTEPTETEPTGQTQTGDGQGGNQLVGTGYSVEVPQQWEDVTDLAR
                     QQNAQADIAMAEPQQSGKFRTNFNVVRPNPIPDSVTDAQLAVQAARELNSVTHNKVSR
                     LDGPDFDGEHSLGQTSKASASGFSVTLVQYLVRHDGKVYATTMTFDSTRAAQAQQVLD
                     DIVDSWSWTG"
BASE COUNT         1407 a         3224 c         3283 g         1542 t
ORIGIN      
        1 aggtccaggt cggcgaactc cggtcccagc tcgtcggaca gctgtgactt cgcctgggtg
       61 agcatctggc gggccgagcg cagcatccga cctgcctgcc gcgcgacgtc agggagcttg
      121 tcgggcccga aaacgaacag cgcgacgaca gccagcacga gaaactccgg caagccgatg
      181 tcgaacattg ctcaagcctt ccacgtcgga aagagggctg aacccgtcat cacacatctg
      241 attcggcacc gaagccggtt cagcgtccgg cgggtgtcaa acccagctgc atcccggcga
      301 gtccgcgccc cttggcggtg agcacggttg cgatatcggt gaggacctgc gcgggcggcg
      361 tgtccggcgc tgccaacacg atcggttcac ccgcgtcgcc acccgcacgg agccgtgtgt
      421 cgagggggac ctcgccgagc aggggtacgt cgtacccgaa ccgcttcgac aaggtcgcgg
      481 cgacctgcgc accaccgccg gagccgaaga cgtcgatccg gtgatcgggc ccgcagtgcg
      541 gacagaccag gtagctcatg ttctcgatca cgccggttac ccgctggtgc atcatcgagg
      601 ccatcgtccc ggcgcgttcg gccacctccg aggcggccgc ctgcggggtg gtaacgacca
      661 gcacctccga gctcggcagg tgctgtccga tcgagatcgc catgtcaccg gtgccgggcg
      721 gcaggtcgag cagcaggacg tccaggtcac cccagtacac gtcggtcagc atctgcacca
      781 acgcccggtc cagcatcggt ccacgccacg cgacgacctg gtcgcggcgc ggcttgagca
      841 tcccgatgct gatcacgctg agtcccaagg ccggaacggg catgatcatg tcctcgacct
      901 gcgtcggacg ctcgtcggcc acacccaaca ttgccgggac cgagtgaccg tagatgtcgg
      961 cgtcgaggat gccgaccttg cgaccgctcc ggaccatcgc catcgcgagg ttgacggtga
     1021 tcgaggactt gccgactccg cccttcccgg acgccaccgc gagcaccttg gtgagtgagt
     1081 cgggacgggt gaacgggatc tccttggcag gttggccgcc gcgaaggtcc tgctgcagct
     1141 gctggcgctg ttcgtcgctc atcacgccga ggcgtacgtc gacggtgctc acacctgaca
     1201 cgttgctcac cgcggcagtc acgtcgcggg tgagggtctc gcgcatcggg cagccggaga
     1261 cggtcagcaa caccgtgacc gcgacttgcc cgtcgtcacc gacggtgacg tcttcgacca
     1321 tcccgagctc ggtgatcgga cgacggatct ccgggtcgtt gacggtggcg agcgcagcct
     1381 gcacccgctc gagcaggggg gacgtcatgc gtccaggcta cgtgtctcgg cgaccggctc
     1441 ggcatcgcgg tcgtcctgcg cccgatcgtc gaggtcggac aacagcgacc gcagctcgga
     1501 ccggacgaag tcgcgcgtcg cgacctctcc gacggccatc cgcaacgacg ccatctcgcg
     1561 ggcaaggaac tccatgtcgg cgtgcgcccg gacgtcctgc tcccggtcct gctcggcgac
     1621 aacgcggtcg cgcgcctcct gccggttctg cgccagcagg atgagcgggg cggcgtacga
     1681 cgcctgggtg gagaagatga ggttcagcag gatgaacggg tacggatccc actccaatcc
     1741 ccagacgccg acgaggttga tcgtgatcca caagatcacg aacaccgtca tgtaggcgat
     1801 gaactgtgcc gtccccatga agcgtgcgaa gctctcggcg aagcggccga agcgctccgg
     1861 gtcgtacgtc ggccggcgga cgagctgccg tctcgtctcc cgcgggacgt cgagccgcgc
     1921 ggtgcgcgac gtcgtacgac ggctcgcctg gcgctcctcg tcgcggacgt cctcacgcgc
     1981 cacgactggc tccctccggg ctcgccgcgg cggtcacggt ggagtcgtcg gcgcgctgtc
     2041 gccagttctc cgggagcagg tggtcgagga cgtcgtcgac cgacacgacg ccgagcaggt
     2101 ggccgtcctc gtcgacgacc gggctcgcga ccaggttgta gtaggcgaga tggcgggcca
     2161 cctcctccag tggcgcatcc gcacgaaggg cttcgagatc gctgtcgagg acgccggaca
     2221 cgagacccga gggtgactcg cgcagcagtc gctggaagtg tccgacaccg aggaaccggc
     2281 cggacggcgt ctcgagcggc gcgcggcaga cgtacaccat ggccgccagc gacggggtga
     2341 gctcgctgtg cctgatgcgt gcgagcgcct ccgcgaccgt ggcgtccggc gggaggatca
     2401 ccgggtcggt ggtcatcagg ccgccggccg tgtgctcctc gtaggtgagc aggcggcgta
     2461 cgtcctcggc ctcgtcgggt tccatcagtg ccagcagcgc ctcggcggtg tcgggcggga
     2521 gctcggcgat gaggtcggcc gcgtcgtcgg gggacatctc ctcgaggatg tcggcggcgc
     2581 gctcgttctc gagctggctg aggatctgga cctggtcgtg ctcgtcgagc tcctcgagga
     2641 cgtcggccag ccgctcgtcg tcgagtgccg cggccacctc gcctcggcgc ttcggggaga
     2701 ggtcgtggat catgttggcc aggtcggctg cgcgcatgtt gtcgatcgcg gcgagcatgt
     2761 gcgtggcgcc ttggccggct tcgctctcgg cgagtccggc gacctctgac cactcgaaga
     2821 cgtgggtctg gcctctgcgc ccgaaccgct tcgcgccctg ctgcaccgcc acccgtgaga
     2881 tcacccagtc ccgggtgcgc gcctgttcca tcgcgatgtc gtagaccgtg ccctcgaccc
     2941 cggtctccgt gatcgtcacc ttgcgatcga gcagctcgcc gagcaccagg gtttcgccgg
     3001 cgcgctgctc gaagcgccgc atgttgacaa ggccggtcgt gatgacctgg cccgagtcga
     3061 cgctggtgac ccttgtcatc ggcacgaaga tccgtcgtcg gccgagcacc tcgacgacga
     3121 gcccgagcac gcgcggctgg acgttctcgg cccggaccgc tgccacgacg tcgcggacct
     3181 tgcccacctg gtcgccgttc gggtcgaaga cgagcaggcc ggcgagccgt gcgatgtaga
     3241 cgcgggtcgg ggcgacgctc acgccgaaag gctaccgttg acgcttcgcg ttcccgcccc
     3301 gtacgcgcct ggccaccacc gttgtggggt tgcgatgttt acagcccgcc cggcctgtca
     3361 gcatcgcaaa tcctcgatgc aggcagtggg tttgggatgc gcaccctctg ggcgggtggg
     3421 gctcataggc tcgaggtatg aacgctccgt cgacgcacac tcccagcgaa cgagccgcca
     3481 cgaacgccga gacgaccgac ctgatgcggc cggtggaggg gcgactgcgc ttcgaggtcg
     3541 tcgacgtgtt cactgaccgt ccgttcgcag gcaacccgct tgccgtggtg tacggcgcgg
     3601 agggtctggc caccgagcaa ctgcaggcga tcgcgctgga gttcaacttg tccgagacga
     3661 cgtttccggt cgtggcgagc gacgaggacc gcgcggcggg tgccgactac cgggtccgga
     3721 tcttcacgcc aaccgaggag atcccgttcg ccggacatcc cacgttgggc actgcctggt
     3781 cgcttcgaca ggcgggtctg atcgacccgg gcgcgcgcgg gcagcgctgc ggggcgggtc
     3841 tcatcggcgt ggacgtgccc gaggatcctg ccgggaacgt cgagctgacg gcggcaccca
     3901 gagacgagcc ccgcgcgctg tccgctgccg acgtcgccac cgtcgccgcg ctggtcggtc
     3961 tcaccgccga tgacgtgacc ggctcggcat acacggccgg atgcggcctc agctggatct
     4021 tccttccggt cacgcgggag gccgtcgcga gggctcggcc aggctcgcag ccgatcagcg
     4081 aggcgggact cgacctcggc gcgctcgtcg acccgctcga cggagtcgac gtgtacgccg
     4141 ttcgcgacgt caacgggaac gacgacgtca acggcaacga cggcgtctcc gtggagtcgc
     4201 gcgtcttcgt gcccggcttc ggcatccccg aggacccggc aaccgggtct gcggccgctg
     4261 gtctcggtct ggttctggcc gcctcaggag tggcggctgc cgtcggcacc acggcgtaca
     4321 cgatcagtca gggagttcag atgggtcgac cgtccaccgt gcacgggcga gtcgaggtga
     4381 ccggaggccg ggcgacacgc gtccacgtgg ccggctccgt cgtacctgtc tccaccggca
     4441 cgatcgccat cccgtgagcc gagttgtcag aagtggatcg agccataggt cgatccactt
     4501 ctgacaagtc ggtcaggaca gggggcggcc gcggtggccg cgcttgtgca gcacccaggg
     4561 cggcttgccg gcggtcgtgg ccgccgttgg gacaggtgcc gcgcgatggc tctcggacgg
     4621 gtacgacccg ggcttctccg agatcgtgcc ggcgggccgc agccggtaga tcaccgcggc
     4681 acctgcagcc caccgctggg gcgccgactc ggcgtcgcgc aggttgagcc gcccggtcgc
     4741 cagcgcagcg gtcgccgcct cccagtcgtc cgcctccggg agcaccctgg acgcgtaggc
     4801 gtcgaacgtc acgagccggt gcacggtgtc cttgctccgc acgagcacgg acaccggtgc
     4861 atgctcctcc cacccggggt cgggctgctc ccccgatccg gtcaggacgt atgccgtgcc
     4921 gtccacccag gcgtgccaga acgcccggcc cgccgggacg tccggcgtcg tgacccagat
     4981 gaggccggac ttcttcatgg cctcgtccac gagcgcctcg tccgccggat ccaggctgac
     5041 tgtcggctcc ggatcgggcg gggcgacctc ctcggccggt tcgacctcct cggccggttc
     5101 gacctcctcg accgcatcga gatctgctgc gggatcgggc gacgccgggt gacggccatc
     5161 ctgcgcggcc tcgtccacct ccgaggcggc cgcctcgtct gaccattcct cctggtcggc
     5221 gccgacgccg tcgtcttcga cgagtgggcg ggactggtcc tcgaacacct ggtcgatgaa
     5281 cgccctcggc gcctcgtcgt ccggctcgcg tgcgtcaccc acgtgtcgaa gcgtgccaca
     5341 gccggtggtg agatggcagc gtgaccggcc ccccgtcgac accgccgact ccgtcgatgc
     5401 caccgactcg gtcgacggca tcggccgcgt cgccggcggc cggcccgcgt accgctgtcg
     5461 gtggctcggc gagcacggtg gcgttgattg tggtcggcgt tgtcggcgtc tcgatgtcgg
     5521 gtccgatcat ggccgccgcc accgcggttc ccgctctggc gatgtcgttc tggcgcaacg
     5581 gcctcggcgc cctggccgtg ctgccgttcg cgctcgtcag cggaagagct gagctcgccg
     5641 cgctgtcgcg ccgccagctc cgcctgggcc tgctggccgg cgccatgctg gcggcgcact
     5701 tcggtacctg ggtgtcgtcg ctgcggctca cgtcggtcgc gtccgcgacc gcgctggtga
     5761 gcctgcaggt ggcgtgggtc gtcgtgctca cccgccttct cggccagccc gtcgtccgcc
     5821 gggtctggct cggcctcgtg ctggccctgt cgggtgtgat cgtcatctcc ggtgtggact
     5881 tctctgtctc gaccgaggca ctgacgggtg acgcgctcgc tttcgccgga gcgttcttcg
     5941 ccgcggtcta caccatggtc ggtgggcggg tccgggaaga gctgagcacg acgacgtaca
     6001 cgttcgtctg ctactcgacc agtgccctgc tcctgctcgc ctgctgtgtg gtggggcggc
     6061 tgcaggtcgt ggggtacgac gcgaagagct ggctgctcat cgtcggcgtc accgtctcgg
     6121 cccaactgct cggacacacg gtcttcaacc acctcctcgc gacgatcagc ccaaccatcg
     6181 tgtcgatggc gctgctgctc gaggtcccgg gcgctgccct gctcgccgcg gtcttcctct
     6241 cccaggcccc gccgaccgcc gtgtacgccg gcctggccct gatcgtcgca ggtctcgcga
     6301 tcgtggttgg tgcgcgttcg gtgtcgtcgg cgccccgcga agcgccggtc gactgaggcg
     6361 gtcgcggagg agacgtcgcg gcgccgtagg ctcggcccgg acggcgggac cggacgtcga
     6421 ggacgtcacg gacgacagga ggagagatgg ccatgcctga agctccgcga ccacgccggt
     6481 cgtgcctggc tgtccctggg tccaacccgc ggttcctcga aaaggccaaa ggactggacg
     6541 ccgaccaggt cttcctcgac ctggaggact cggtcgcccc gatcgccaag gccgacgcac
     6601 gcggcagcgt catcgaggca ctgaactcgg gggggtgggg cgaaaaggtc cgcgtcgtcc
     6661 gagtcaacga ctggacgacc gagtggacct accgcgacgt gacccaggtg gtcgaagcgg
     6721 ccggggcgaa cctcgactgc gtcatgctgc cgaaagtgca gagcgccgat caggtcgtcg
     6781 ccctcgacct gctgctcacc cagatcgaga agaccgtcgg cctcgagctc ggccggatcg
     6841 gcatcgaggc gcagatcgag aacgcgcgcg gcctcgtcaa cgtcgacgcc atcgtcaccg
     6901 cgagcccgag agtcgagacg gtcatcttcg gtcccgctga tttcatggcg tcgatcaaca
     6961 tgaagtcact cgtcgtcggt gaacagccgc cggggtacga cgtcggcgac gcctaccact
     7021 acatactgat gcggatcctg atggccgccc ggacacacga caagcaagcc atcgacggcc
     7081 cgtatctgca gatccgcgac gtcgacgggt tcaagcgtgt cgccgcgcga agcgccgccc
     7141 tcgggttcga cggcaagtgg gtgctccacc ccgaccagat cgccgccgcc aacgaggtgt
     7201 actcgccact acagtccgac tacgaccacg ccgagaacat cctcgacgcc tacgcgcact
     7261 ccacctccga aagcggcggc gccaccggag cggtcatgct tggcgacgag atgatcgacg
     7321 aggcatcccg caagatggcg ctcgtcgtct cggcgaaagg tcgcgcggcc ggtatgacgc
     7381 gtactgacgt ctggacgcca ccgaacgact gaccccacct tgcgtccgtg cttccggccg
     7441 ccagggcggc cggaagcacg gacgcaaacc acgtagcctc gaagcgagtg ccgagcagga
     7501 aggcatcgac atgggtcgcc tctgcgagac cgacggtctg accgatgtgc agaccgagat
     7561 cctcaagacc gtccgcgagt tcgtcggcaa ggagatcctg ccggtggcga accagctcga
     7621 gcacgccgac gagtacccgg agaagatcgt cgagggcatg aaagacctcg gcttgttcgg
     7681 cctgacgatc cccgaggagt acggaggtct gggcgagtca ctcctcacct acgccctcgt
     7741 cgtcgaagag atcgctcgcg gctggatgag tgtctccggc atcgtcaaca cccacttcat
     7801 cgtggcctac atgctgatgc agcacgggac ggacgagcag aagcggaggt acctcccgca
     7861 gctggcgacc ggcgacgtgc gcggctcgtt ctcgatgagc gagcccggct gcggctcgga
     7921 cgtgtcggcc atccgcacga gggctgtgag ggacggcgac ggcttcgtcg tcaccggcga
     7981 gaagatgtgg atcacgaacg gcagcacgtc caacctgctg gcggtgctcg tcaagaccga
     8041 cgagggtccg gagtacgccg ggaagccgca ccgcaacatg acgacgttcc tcgtcgagaa
     8101 ggaccccggg atcggtgcac aggcagctgg cacgctgacg atcccgaaga agatcgacaa
     8161 gatgggctac aagggcgtcg acacgacggg ggccgtcttc gacggctacc ggataccgtc
     8221 tgaccaggtg ctcggaggac agaccggtcg cggcttctac cacatgatgg acggcgtcga
     8281 ggtcggccgg gtgaacgtcg ccgcccgcgg ctgtggcgtc gcctggcggg cgttcgagct
     8341 gggcgtcgcg tacgcgcagc agcgcgaggc gttcgggagg aagatcgccg agcaccaggc
     8401 ggtgctcttc cggctggccg agatggcgac gaaggtcgag gcggcgcacc agatgatggt
     8461 gcgggcggcc cgcaagaagg actccggtga gcgcaacgac ctcgaggcgg gtatggcgaa
     8521 gtacctcgcc tcggagtact gcgcccaggt cgtggaggac tcgttccgga ttcacggcgg
     8581 ctacggctac tccacggagt acgagatcga gcgcttgtac cgcgaggcgc cgatgcttct
     8641 gatcggtgaa ggcacagcgg acatccagcg gatgatcatc ggccgacggc tgctcgagga
     8701 ctacaagctc tgacgctgct tcccgccgtc acctgtcgag acgtgacgtg atgctcgccg
     8761 gggagcgttc agccggtcca gctccagctg tcgacgatgt cgtcgagtac ctgctgcgct
     8821 tgagctgctc gcgtgctgtc gaaggtcatc gtggtggcgt acaccttgcc gtcgtggcgc
     8881 acgaggtact gcacgagggt caccgagaag ccggaggctg atgccttcga ggtctggccg
     8941 agcgagtgct cgccgtcgaa gtccgggccg tcgagcctgc tcaccttgtt gtgcgtgacg
     9001 ctgttgagct cgcgggcggc ctgtacggcc agctgcgcgt cggtgacgga gtccgggatc
     9061 gggttgggcc tgaccacgtt gaagttggtc cggaacttcc cggattgctg cggctccgcc
     9121 atcgcgatgt cggcctgggc gttctgctgc cgggcgaggt cggtgacgtc ctcccactgc
     9181 tgcggcactt cgacgctgta gcccgtaccg acgagctggt tgccgccttg tccgtcgccg
     9241 gtctgcgtct gcccggtggg ttcagtctcg gtcggctcgg tcgactcgct cggctcggtg
     9301 gtggtcggcg gcgtcgagct gtccggttcg gtctgcgtgg cggaggacac ggtgtcgtta
     9361 ccgtcgttcc cgtcgttgcc gctctgttcg gtgtccgacg ccgagctgca cgcggagacc
     9421 accgccacgc acgccagcgc tgccacccag cctcgt
//
LOCUS       DATBFZ010000513         3326 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_4509120, whole genome shotgun sequence.
ACCESSION   DATBFZ010000513 DATBFZ010000000
VERSION     DATBFZ010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 3326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3326
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4509120"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..859
                     /locus_tag="VH419_15790"
     CDS             <1..859
                     /locus_tag="VH419_15790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY7045133.1"
                     /translation="LIAPYGFAQSSADGVRFPKLGHPSADHIFGTNDLFYDVFSRIIW
                     GARTEIEVVLLSILLCVIVGVPVGLISGYYGGWLDRILVLIMDAMFAFPSFLLAIVFA
                     FLLTNLFGGSVIAVALSLTAIYVPQYFRVVRNTTVSAKEHTYIEAAKALGARDGVVMR
                     RYLFGNVVQSVPVLGTLNAADAILTLAGLGFLGLGIQPTDAAEWGYDLNRALDDAGSG
                     VWWTGLYPGLAIVLLVLGLTLVGEGLNETLNPTLRRRRLLPVMMPKREKVDLAEQAID
                     LNQGDRVQR"
     gene            856..1917
                     /locus_tag="VH419_15795"
     CDS             856..1917
                     /locus_tag="VH419_15795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY7045134.1"
                     /translation="MTAQTTQIVRTDTASPVISARDVRVWYGTTRGAIRAVDGVSFDL
                     APGETLGLVGESGCGKSTLGRGLMGLLPEGAKRDGEVLFKGKDILSMSAKESYRLRGS
                     ELGMIFQEPLTRLNPLMRISEHFEETIEQHEPDLSKLELRNRALDVLRLMGIPPTRYK
                     SYPHEFSGGMRQRLMIALALVLRPSFIVADEPTTALDVLVEAQIIAILNDLRRNFDTS
                     LLLITHNLGIVAESCDRVAVMYAGRIVEQGMVREVFGKPQHPYTRELLRSTISLSTTG
                     LNFIPGAPPDLVDPPSGCRFHPRCPEAMKVCVDHDPVLTRSSGGTAVECWLHGPDELI
                     PPGGTEPVEQKEISLADEA"
     gene            1901..3196
                     /locus_tag="VH419_15800"
     CDS             1901..3196
                     /locus_tag="VH419_15800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY7045135.1"
                     /translation="MPTKPDPRVDRLASSETVVDIADLSVHFSLQGSSFARLFGRDTG
                     TVKAVDGIDLTLRKGEVVGLVGESGSGKSTLGRALLGLAPATTGSITFRDEDIAKMSN
                     RALRQIRQDVQMIFQDPNAALNPAMTIEQAVGDPLRIHGVRSVEERRTKVIDALERVG
                     LSPVELFMNKYPRDLSGGQKQRVVIARAIIMGPTVLVADEPIAMLDMSVRAKILQLML
                     DLRDQLDLTYVYITHDLASAKFFCDRIAIMYLGRIVEIGETSEIFDAPKHPYTKALLA
                     AIPDPDPERTMPRDLLKGEIPDAARPPRGCAFHPRCPEAFEKCGWEARDLVATLEAHW
                     LKSGISTFDTERAQIGNLDELTAQGDGPAHLSAAGEGSPERLQEILEEVRAERADARF
                     WKGVESVEPDTTGVVVSFHEGEDPKLRPAGGVEVACHLY"
BASE COUNT          544 a         1081 c         1125 g          576 t
ORIGIN      
        1 gttgatcgcg ccctacggct ttgcccagtc cagcgccgac ggcgtgcggt tcccgaaact
       61 gggtcacccg agcgcggacc acatcttcgg caccaacgac ctcttctacg atgtcttctc
      121 ccgcatcatc tggggggcgc gcaccgagat cgaggtcgtg ctgctttcga tcctgttgtg
      181 cgtgatcgtc ggtgtgccgg tggggctcat ctcgggctac tacggcggct ggctggaccg
      241 catcctggtg ctgatcatgg acgcgatgtt cgcgttcccg tcattcctgc tggcgatcgt
      301 gttcgccttt ctgctgacca acctgttcgg cggcagtgtc atcgctgtcg cgctgtccct
      361 gacggcgatc tacgtcccgc agtacttccg cgtcgtgagg aatacgaccg tgagtgcgaa
      421 ggagcacacc tacatcgagg cggccaaggc gctcggggcg agggacggtg tcgtgatgcg
      481 gcgctacctg ttcggcaacg tcgtgcagag cgtgccggtg ctgggcacgc tcaacgccgc
      541 ggacgccatc ctgacgctcg cggggctcgg tttccttggt ctcgggatcc agccgacgga
      601 tgccgcagag tgggggtacg acctcaaccg tgcattggac gacgcgggat ccggcgtctg
      661 gtggacggga ctgtatcccg gcctggcgat cgtgctgctc gtgcttgggt tgacgctcgt
      721 cggcgaaggg ctgaacgaga ccttgaaccc gacgctgcgc cgacgccggc tgctgccggt
      781 catgatgccg aaacgcgaga aggtggacct ggccgaacag gcgatcgacc tcaaccaggg
      841 tgacagggtc caacgatgac cgcccagacg acgcagatcg tccgcaccga caccgcgagt
      901 ccggtgatct cggcacgtga cgtccgtgtt tggtacggca cgacgcgtgg cgcgatccgc
      961 gcggtcgacg gcgtcagctt cgatctcgcg ccgggagaga cgctcggcct ggtcggtgag
     1021 tccggctgcg gcaagtcgac cctcggacgc ggcctcatgg gcctgttgcc cgagggcgcc
     1081 aagcgtgacg gcgaggtgct cttcaagggc aaggacattt tgtcgatgag cgccaaggag
     1141 tcctaccgac tgcgcggtag tgagctcggg atgatcttcc aggagccgct cacccggctc
     1201 aacccactga tgcgtatctc cgagcacttc gaggagacga tcgagcagca cgagcccgac
     1261 ctctccaagc tggagctccg caaccgtgcc ctggacgtgc tgcggttgat ggggattccg
     1321 ccgacccggt acaagtccta tccgcacgag ttctcgggcg ggatgcggca gcgcctgatg
     1381 atcgcactgg cgctggtgct gcgcccctcg ttcatcgtgg ccgacgagcc gacgacggcg
     1441 ctcgacgtgc tggtcgaggc acagatcatc gcgatcctca acgacctacg gcgcaacttc
     1501 gacacgtcgc tgctcctgat cacccacaac ctcgggatcg tcgctgagtc ctgcgaccgc
     1561 gtcgcggtga tgtacgccgg ccgcatcgtc gagcagggga tggtccgcga ggtgttcggg
     1621 aagccccagc atccgtacac ccgagagctg ctcagatcga cgatcagcct gtcgacgacc
     1681 gggctcaact tcatcccggg cgcaccgccc gacctcgtgg acccaccgtc cggctgccgg
     1741 ttccatccgc ggtgtccgga ggcgatgaag gtctgcgtcg atcacgaccc cgtgctgacg
     1801 cgctcgtccg gcgggactgc cgtcgagtgc tggttgcacg gtccggatga gctcatccca
     1861 ccgggcggga ccgagccggt cgagcagaag gagatcagcc ttgccgacga agcctgaccc
     1921 gcgggtcgac cggctcgcga gcagcgagac cgtcgtcgac atcgccgacc tgtcggtcca
     1981 cttcagcctg caggggagtt ctttcgcgcg gttgttcgga cgtgacacgg gcaccgtgaa
     2041 ggcggtggac ggcatcgacc tcaccctgcg caagggagag gtggtcgggc tggtcggcga
     2101 gagcggcagc ggcaagtcga cactcggccg tgcgctgctg ggccttgccc cggccacaac
     2161 cggctcgatc accttcaggg acgaggacat cgccaagatg tcgaaccgcg cccttcggca
     2221 gatccgccag gacgtgcaga tgatcttcca ggacccgaac gccgcgttga acccggccat
     2281 gacgatcgag caggcggtcg gcgacccgct tcgcatccat ggggtcaggt cggtcgagga
     2341 acggcgtacc aaagtcatcg acgcactcga gcgcgtcggg ttgtcgccgg tcgagctgtt
     2401 catgaacaag tacccgcgcg acctctccgg tgggcagaag cagcgggtcg tcattgcgcg
     2461 cgcgatcatc atggggccga cggtgctggt cgccgacgag ccgatcgcga tgctcgacat
     2521 gagcgtgcgc gcgaagatcc tgcagctgat gctcgaccta cgtgaccagc tcgacctgac
     2581 gtacgtctac atcacgcacg acctggccag cgcgaagttc ttctgcgacc ggatcgcgat
     2641 catgtacctc ggccggatcg tcgagatcgg cgagacgtcg gagatcttcg acgcccccaa
     2701 gcacccgtac acgaaggcgt tgctcgcggc catccctgac cccgatcccg agcggacgat
     2761 gccgcgggac ctgctcaagg gcgagatccc ggacgcggcg cgtccaccac gcggctgtgc
     2821 cttccacccg cggtgcccgg aggcgttcga gaagtgtggc tgggaagccc gtgacctggt
     2881 ggccacgctg gaggcgcact ggctgaagag cggcatctcg acgttcgaca ccgagcgagc
     2941 tcagatcggc aacctcgacg agctgaccgc gcagggcgac ggccccgcgc atctcagcgc
     3001 ggctggggaa ggtagcccgg aacggcttca ggagatcctc gaggaagtgc gtgccgaacg
     3061 cgccgacgcg aggttctgga agggcgtgga gtccgtcgag cccgacacca ctggagtcgt
     3121 cgtcagcttc cacgagggcg aggacccgaa actccgaccc gccggcggcg tcgaggttgc
     3181 ctgccacctg tattgagccg ctgcgcgtac gttgacggtt tgcctggcta gacggtatgg
     3241 agcgtgcctg cggttggtga gtggaggttc ctcgctgagc tcagtgtctg ccggtggccc
     3301 ttggtgtcaa cgtcgttcat gctcgc
//
LOCUS       DATBFZ010000514        12557 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_5492869, whole genome shotgun sequence.
ACCESSION   DATBFZ010000514 DATBFZ010000000
VERSION     DATBFZ010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 12557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12557
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5492869"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..107)
                     /locus_tag="VH419_15805"
     CDS             complement(<1..107)
                     /locus_tag="VH419_15805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HEY7045136.1"
                     /translation="MLLSDRDIRAEIDEKRVLLEPYDAAMIQPSSVDVRL"
     gene            complement(193..1020)
                     /locus_tag="VH419_15810"
     CDS             complement(193..1020)
                     /locus_tag="VH419_15810"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY7045137.1"
                     /translation="MAILFALLSSVMWGSSDFLGGLRSRTRPPVAVVAGSMFCGLVVI
                     SIVALAYGAYDDPTGWIPWSMMSGVVGATGLVAFYGALAGGTMGVVSPIAALGVAVPV
                     IIGFARGETPSTIQTVGLVVALTGAIATSGPELSGRASARSVLLAAFAGVCFGLVFVA
                     VDQGAESSPLMTLIGMRTTSIVMFGIVALVARNIGGLDRTDVLPLVVIGSFDVFANLM
                     FALATNRGYVSIVSVLGSLYPVMTVLLARAFLHERMRTIQGLGVAVTMLGVAFISAG"
     gene            1176..1246
                     /locus_tag="VH419_15815"
     tRNA            1176..1246
                     /locus_tag="VH419_15815"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1208..1210,aa:Gly,seq:ccc)
     gene            complement(1272..1661)
                     /locus_tag="VH419_15820"
     CDS             complement(1272..1661)
                     /locus_tag="VH419_15820"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEY7045138.1"
                     /translation="MLRVLDSTVLIDYLRGRPAVERVASMRSRGDTAATTGINVEEVV
                     RGLRDHEMEAAQRLFSGLVVLPIDARAGWRAGTWRREFAARGVTLWQADCLLAATAMV
                     HGATLVTGNPKDFPMEGLDVDHWPVGG"
     gene            complement(1666..1905)
                     /locus_tag="VH419_15825"
     CDS             complement(1666..1905)
                     /locus_tag="VH419_15825"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HEY7045139.1"
                     /translation="MRMHIELDDELVAQLDELAGHRGRSAFVRRAIESAVRHELRWTD
                     IESAAGAIEDEGHDWDADPAAWVRQQRRADPRRAG"
     gene            complement(1956..2705)
                     /locus_tag="VH419_15830"
     CDS             complement(1956..2705)
                     /locus_tag="VH419_15830"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012920951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY7045140.1"
                     /translation="MSRRVLVTGAARGIGRAVAEAFAGTGARVAVHYGRSADQAAEVV
                     ASLPGAGHVLVQADMSEAEDVRRAVDSAAERLGGLDALVNNAGVFLAHPPMETSWEDW
                     QAAWTATLQTNLVGAAHATFAAIPHLVSAGGGAVVNVSSRGAFRGEPNNPAYGASKAG
                     LNAFGQSMARALAPYGIWVGTVAPGFVDTDMARDVLEGPEGDGVRGQSPFGRVARPDE
                     VAAAVLWLASPAAKFASGAIIDVNGASYLRS"
     gene            complement(2702..3901)
                     /locus_tag="VH419_15835"
     CDS             complement(2702..3901)
                     /locus_tag="VH419_15835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase-like protein"
                     /protein_id="HEY7045141.1"
                     /translation="MDFDVERVRADFPALEDGVAYFDGPGGSQVPRQVADAMAAAMVS
                     GISNRGTVTRAELRAEAVVRNAREAVADLLGAVPGGVVFGRSMTQLTFDVAHTMAKNW
                     SAADEIVVTRLDHDGNVRPWVVAAERAGATVRWVDFDPDTAELTVADVEAQLSDRSRL
                     VAVTGASNLLGTRPDIPAITAAVHAAGAMAYVDGVHLTPHAPVDVAALGADFFVCSPY
                     KFFGPHHGVLVADPGLLETLDPDKLLPATSAVPERYEQGTLPYEILAGTTATVDFIAG
                     IASEAASRRERVLESMASVEAYEDNLLARLVEGLASQARVRLHGAPKRRTPTLLFSVD
                     GVEARTVYEALAARGVNAPAGSFYALEASRRIGLGDTGAVRAGLAPYTTTQDVDRLVA
                     GVAEICR"
     gene            4037..4435
                     /locus_tag="VH419_15840"
     CDS             4037..4435
                     /locus_tag="VH419_15840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7045142.1"
                     /translation="MTDRVHVTMDLELFVDDMDAMRKAAYERMKSAWSSDDAFPVESP
                     DDVALDQAVASLLADALPISVPGARRSQLAVNTAEPERLDDGEHADENGEQDDQTEDS
                     DDSDEGDQGSDDDDQGSGDSDEGERRSSQD"
     gene            complement(4454..4780)
                     /locus_tag="VH419_15845"
     CDS             complement(4454..4780)
                     /locus_tag="VH419_15845"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020385065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEY7045143.1"
                     /translation="MSEAADAVRPGDVDKAVWAELTRLRESIDNLDAALVHVLAERFR
                     CTQAVGLLKAEHDMPPADPAREAQQIARLRRLAQEARLDPEFAEKFLNFIIAEVVRHH
                     EAIAKP"
     gene            complement(4944..5402)
                     /locus_tag="VH419_15850"
     CDS             complement(4944..5402)
                     /locus_tag="VH419_15850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEY7045144.1"
                     /translation="MRVSAKSDYALRALIEIATRGDGGPVSAEELGRLQDIPHGFLQA
                     ILADLRRAGIVMSVRGQSGGWRLARGSDEVSVADIIRAVDGPLVSVYGLRPEAVTYNE
                     SATVLQLVWIAARASLRDVFEQVTVEMLATGKLPNDVVRLTKTEDAWRSH"
     gene            5589..5987
                     /locus_tag="VH419_15855"
     CDS             5589..5987
                     /locus_tag="VH419_15855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7045145.1"
                     /translation="MITVDADTIRLFLHVLAATVWVGGQIVLAALLPTLRAAGAAVPG
                     AAARAFNRVAWPAFGVLVLTGVWNVVAEGDRGPAYEHTLMLKYVLVLGSGVTAFVHMK
                     ARSRAMMAVFGALTGLFALAALFVGIMLAG"
     gene            complement(6165..6587)
                     /locus_tag="VH419_15860"
     CDS             complement(6165..6587)
                     /locus_tag="VH419_15860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7045146.1"
                     /translation="MKPASQGRTAPCGRTEALARLDQARKFQEVAMMVGAEADSVAPS
                     ASVAAASAVLAGIAAVDAACCASLGRRSRAKGHKAAVELVRQVEPGGANAAKNLDRLL
                     DLKDSAHYGVINVSGANLKTCLRTAGTLVEFADTVVRS"
     gene            complement(6584..7210)
                     /locus_tag="VH419_15865"
     CDS             complement(6584..7210)
                     /locus_tag="VH419_15865"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEY7045147.1"
                     /translation="MDWSEPAAAVVPSLDGAVLDVLASTTRPLTGRDVARLARRGSPA
                     GVSNVLNRMVDQGLVTVSSAGMSNLYTLNHDHVATPAVLALQDLRGEAFARIRKAMNS
                     WDPPPVAAAVFGSAARGDGGVDSDVDLLVIRPADTRDDDPNWSGQVDALAQSVRRWTG
                     NAASIIQLTPAEMTDMLDRKERIVQELRKVKVALTVTDVLDLGAEEFE"
     gene            7454..9316
                     /locus_tag="VH419_15870"
     CDS             7454..9316
                     /locus_tag="VH419_15870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015788127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEY7045148.1"
                     /translation="MTHYKSNLRDLEFTLFEVFGRDSVLGTGPFADVDGATAREILHE
                     VDRLAREELAESFASADRQPPVFDPDTHAVTMPEDLKRAFASYMDAEWYRLELPRELG
                     GQPIPASLRWAVAELVLGANPAIHLYSAGPAFAGVLWRNGTERDRRIARHMVDRRWGA
                     TMVLTEPDAGSDVGAARAKAVPLPDGRWRIEGTKRFITSAEHDMADNIIHLVLARPVG
                     VEGAGGPGTKGLSLFIVPKHDFDLETGELTGRRNGVHVTGVEHKMGIKASATCELTFG
                     AEEPAYGWLLGEVHDGIAQMFQVIENARMMVGTKAMATLSTGYLNALEYAKARIQGPD
                     LTRSADKTAPRVPITHHPDVRRSLLTQKAFSEALRALVIYTATYQDTAALARANGQTD
                     ELAESVNDLLLPIVKGYCSERAWVVLGTESLQTLGGSGFLEDYPIEQYVRDSKIDTLY
                     EGTTAIQGMDLFFRKIVKNQGRALRALAAEITGFLQSEAGNGRLKDERELLANGLADA
                     EALVGSLVGHLMSSSPDVEGGDITNVYKAGLNTTRLLMALGDVICGYLLLLQAEVALG
                     RLAGEVSPRDELFYEGKVAAAQFFARTVLPKVTAERAIAESTDLMPMELDEAAF"
     gene            9401..9973
                     /locus_tag="VH419_15875"
     CDS             9401..9973
                     /locus_tag="VH419_15875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7045149.1"
                     /translation="MATPPVEEETIRPRAAWYVVPAALWLTALTLFVIALLSIAHVVK
                     SGVDPIDNHALVDIDTDGLTFYTTGSQSKVTCVLTPPNGSAFSLDLLDVTLNITVDEN
                     SYHAIGVTPDDLPAGRYRFDCTPVLPRTTFGTGPRVDIAALATRALWGIFLPLVLGVI
                     GLVVLIVVIVRRHGAKIRLRQPTPPPPPPY"
     gene            10129..11031
                     /locus_tag="VH419_15880"
     CDS             10129..11031
                     /locus_tag="VH419_15880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7045150.1"
                     /translation="MLATYQHGVVTRRQLTGLSMTPGQIDGQLDARRWRAETPRVVVM
                     HNHQLTRAQLMWVALLTAGSRAALCSHTALECAGFRAFASEAEHVHLLVPRGAKTLDL
                     PNVTVHESRRFKDWELVWFNGLPCTDTARSAVDAAAWQRWPRFAMTMLAAVVQQRLCT
                     AAQLDQALARVGRVRHKQYMRLALRDIAGGAEALGEIDVARMCRRFDLKPPDRQVVRR
                     DPSGRKRYLDCEWNLGNGVIVVLEVDGSHHLTVEHWEADIRRERKIVTSGRLVLRATS
                     AELRLDPHEVATDLRALGVPSCQK"
     gene            complement(11068..>12557)
                     /gene="paaN"
                     /locus_tag="VH419_15885"
     CDS             complement(11068..>12557)
                     /gene="paaN"
                     /locus_tag="VH419_15885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaN"
                     /protein_id="HEY7045151.1"
                     /translation="VPGAAGTVATEKSPFGKPLGVRYPRVTPDAVPALVEAASAGLTA
                     WRDCGIEARVGVGLEVLKRLHARVFELASAVQHTSGQAFVMAFQAGGAHALDRALEAI
                     AYAYIEMTRVPGTAVWEKPGRDAPLRMEKTFTVVPRGLALVVGCNTFPTWNSWPGLFA
                     SLVTGNPVIVKPHPGAVLPLAITVQVCQEVLAEAGYDANIVTLAAEDPADGIAAVLAV
                     RPEVKLVDFTGGSAFGEWLEENARQAIVFTEKSGVNTVVIDSTDSFKGMCFNLAFSLA
                     LYTGQMCTAPQNLFIPGEGIDTDEGHKSVDDVVSGLEGALGKLVGDDARAVEVLGGVV
                     NDGVLERLAAAGSAGTVLIESREVNHPTFEDAVVRTPTLVALDASDAKTYESECFGPV
                     GYLIRTGSTAESIDTFRRTVIDHGAMTASVYSTDDDVVAAMRSAALDAGVALSENLTG
                     QVFVNQSAAFSDFHGTGANPASNATYTDGAFVASRFRFVQSRRHL"
BASE COUNT         2099 a         4325 c         4164 g         1969 t
ORIGIN      
        1 aggcgaacgt ccacactcga cggctggatc atcgcggcgt cgtacggctc gagcagcacc
       61 cgcttctcat cgatctcggc gcggatgtcc cggtcactca gcagcacgcg ccgaccctac
      121 cctttcccgc aactgcgggg acttccggga ttctcgggcg cgaaatcccg gaacttcccg
      181 caactcgcgg gactaaccgg cggagatgaa ggcgacgccg agcatggtga cggcgactcc
      241 caggccttgg atcgtccgca tgcgctcgtg caggaacgcg cgcgcgagga gcacggtcat
      301 cacgggatac agcgacccga gcacgctgac gatcgagacg tagcctcggt tcgtcgcgag
      361 ggcgaacatg aggttcgcga acacgtcgaa ggacccgatc accacaagtg gcagcacgtc
      421 cgtgcggtcc agtccgccga tgttgcgggc caccaacgca acgatgccga acatgacgat
      481 gctcgtcgta cgcatgccga tcaacgtcat cagcggagac gactctgcac cctggtcgac
      541 ggcgacgaag accagtccga aacacacgcc cgcgaacgcc gccaagagca ccgaccgagc
      601 agacgcccga cccgacagct caggaccact cgtcgcgatg gcaccggtca acgccaccac
      661 gagtcccacc gtctgtatcg tcgacggggt ctcgcctcgt gcgaagccga tgatcaccgg
      721 taccgcgacc ccgagggccg caatcggcga aacgactccc atggtgccgc ccgccagcgc
      781 gccgtagaac gccaccagtc cagtcgcgcc gacgactccg gacatcatcg accacgggat
      841 ccagccggtg gggtcgtcgt aggcgccgta cgcgagcgcc acgatcgaga tcacgaccag
      901 tccgcagaac atcgagcccg cgaccaccgc gaccggtggg cgcgtacggc tgcgcagacc
      961 gccgaggaag tcgctgctcc cccacatcac gctcgacagg agcgcgaaga ggatggccat
     1021 ctggctgatt cttccgactg agcggctcag gccgccggga ataactctcc tgctgagacg
     1081 ggtggtcagc aggagagccg ccgaccgacc ccggtagtat cgaccccgct cgtcgcggcg
     1141 ccgctcgttc gctggttcgg cagctgcgac gaagtgcggg tgtagttcaa tggcagaaca
     1201 tcagcttccc aagctgacag tgggggttcg attcccctca cccgctctca agagtctgcc
     1261 ttgccagcca cctatccgcc aacgggccag tgatcgacat ccaagccttc catcgggaag
     1321 tccttggggt tgcctgtcac cagcgtggcc ccgtgcacca tcgcggtcgc ggcaagcaga
     1381 cagtccgcct gccagagcgt cacaccacgg gcagcgaact cacgccgcca cgtgcccgcc
     1441 cgccagccgg ctcgggcgtc gatcggcaac acgacgagcc cgctgaacag ccgctgggct
     1501 gcttccatct cgtgatctcg caggccgcgg acgacctctt cgacgttgat gcccgtggtc
     1561 gccgcggtgt caccacgcga ccgcatcgac gccacccgct ccaccgccgg ccggccgcgc
     1621 agatagtcga tcaggacggt cgagtccagg acgcgaagca cgaagtcaac cggcgcgtcg
     1681 cgggtctgct cgccgctgct gacgcaccca ggcagctggg tcggcatccc agtcgtggcc
     1741 ttcgtcctcg atggcgccgg cggcagactc gatgtccgtc cagcgcagct cgtggcgcac
     1801 ggccgactcg atcgcccgcc gaacgaaggc gctgcggccg cggtgaccgg caagctcgtc
     1861 gagctgtgcg accagctcgt cgtcgagctc gatgtgcatt cgcatgtgct gacgttagca
     1921 catagggagc cgcctagtcg gcggtggtag gtgtgtcagc tgcgcaggta ggacgcgccg
     1981 ttgacgtcga tgatcgcccc gctcgcgaac ttcgccgccg gtgacgcgag ccagagcacc
     2041 gcagccgcca cctcgtccgg ccgggcgacc cgcccgaacg gcgactggcc gcgcaccccg
     2101 tccccctctg gtccctcgag gacgtcgcgt gccatgtcgg tgtcgacgaa tcccggggcc
     2161 accgtcccga cccagatgcc gtacggcgcg agcgccctgg ccatcgactg cccgaacgcg
     2221 ttgagacctg ctttcgaggc gccgtatgcc ggattgttcg gctcgccgcg aaaagcgccg
     2281 cgactcgaga cgttgacgac ggcgccgccg ccggccgaca cgaggtgagg gatcgctgcg
     2341 aacgtcgcat gcgcagcgcc gaccaggttc gtctgcagcg tcgcggtcca ggccgcctgc
     2401 cagtcctccc acgacgtctc catcggcgga tgggccagga acacaccggc gttgttgacc
     2461 agcgcgtcga gccctccgag ccgctcggct gcgctgtcga cggcccggcg tacgtcctcc
     2521 gcttcggaca tgtcggcttg cacgaggaca tggcccgccc cgggaagcga cgccaccacc
     2581 tccgccgcct ggtcggctga gcgaccgtag tgcaccgcaa cccgggcccc cgtcccggcg
     2641 aaggcctctg ccacagctcg cccgatgcca cgcgccgccc cggtgacgag gacccggcgg
     2701 ctcaccggca gatctccgcc acaccggcga cgaggcggtc gacatcctgc gtggtggtgt
     2761 acggcgccag cccggcccgg acggctccgg tgtctcctag gccgatccgc cgcgatgctt
     2821 cgagcgcgta gaagctgccg gcgggcgcgt tgacaccccg ggcagccagc gcttcgtaca
     2881 cggtgcgcgc ctcgaccccg tcgacggaga acaacagcgt cggcgtacgc cgcttgggtg
     2941 caccgtgcag ccggacgcgc gcttgggacg ccagaccttc cacgagccgc gcgagcaggt
     3001 tgtcctcgta ggcctcgacc gacgccatcg actcgagcac ccgttcccgg cgcgaagcgg
     3061 cctccgaggc gatgccggcg atgaagtcca cggtcgccgt cgtgccggcg aggatctcgt
     3121 agggcagcgt cccctgttcg tagcgttccg ggacggcgga ggtggcgggc agcagcttgt
     3181 ccgggtccag cgtctcgaga agtcccggat ctgcaaccag cacaccgtgg tgcggaccga
     3241 agaacttgta cggcgagcag acgaagaagt cggcgcccag ggctgcgaca tccaccggcg
     3301 catgcggcgt caggtggacg ccgtccacgt acgccatcgc cccagccgca tgaaccgcgg
     3361 ccgtgatcgc cgggatgtcg ggcctcgttc cgagcagatt cgacgctccg gtgacagcca
     3421 ccagccgcga acggtcggac agctgcgcct ccacgtcggc gacggtcagc tcggcggtgt
     3481 cgggatcgaa gtcgacccag cggaccgtgg caccggcgcg ttcggcagcg acgacccagg
     3541 gacgtacgtt gccgtcgtgg tcgagtcgcg tcacgacgat ctcgtcggcg gccgaccagt
     3601 tcttggccat ggtgtgcgcg acgtcgaacg tcagctgggt catcgaccgg ccgaagacaa
     3661 caccgcccgg gaccgcaccg agcaggtccg cgacggcctc ccgcgcgttg cggacgacag
     3721 cctcggcacg aagctccgcc ctggtgacgg tcccccggtt cgagatgccg gagaccatgg
     3781 cggcggccat ggcatcggca acctgcctcg gtacctgcga cccacccggt ccgtcgaaat
     3841 acgcgacccc gtcctccagg gccgggaagt ctgcacgcac ccgctctacg tcgaagtcca
     3901 ctcgggcatt ctgcctgccg tacgccgcac cggcgtcgcg ggtgccgtcg tcgaagggcg
     3961 gaccggctcg gctggcagta gcgtcgtgtc agtccgccgg gccgggggac gatccgtgag
     4021 cacgagagga caggccatga ccgatcgagt ccacgtgacg atggacctcg agctgttcgt
     4081 cgacgacatg gacgccatgc gcaaggcggc gtacgaacgg atgaagtcgg cgtggagctc
     4141 cgacgacgca ttcccggtcg agtcgccgga cgacgtggcg ctcgaccagg cggtcgccag
     4201 cctgctggcc gacgcgctcc cgatcagcgt gcccggcgcc cggcgcagcc agctcgcggt
     4261 gaacaccgca gagcccgaac gcctcgacga cggggagcac gccgacgaga acggcgagca
     4321 agatgaccag accgaagact ccgacgattc cgacgagggt gaccaaggct ccgacgacga
     4381 tgaccagggc tcaggcgact ccgatgaggg tgagcggcgt tcgtcgcagg actgacgaga
     4441 ctgccggccg gcgtcagggc ttcgctatcg cctcgtggtg tcggaccacc tcggcgatga
     4501 tgaagttcaa gaacttctcg gcgaactcgg ggtcgagacg cgcctcctga gcgagtcgcc
     4561 gcaaccgcgc gatctgctgc gcctcccgtg ccgggtctgc gggcggcatg tcgtgctcgg
     4621 ccttgagcaa cccgacagcc tgagtacagc ggaagcgctc agccagcaca tgaacgagtg
     4681 ccgcgtcgag gttgtcgatg ctctcgcgga gccgcgtcaa ctcggcccac acggccttgt
     4741 cgacgtcgcc cggccgaacc gcgtcggccg cttcgctcat gtcggcgagc ctatccgccc
     4801 ggtggcccaa caccgtctga ggcggtcgcc cggcccgaca ctgggcaccc caccaccgcc
     4861 ggagcggacg gacccttgtg accgatcgat ctcgcggtcg tcgtcccatg cttcaacgaa
     4921 gagcaggcca tcgaccagtg ctctcaatgt gagcgccagg cgtcctcggt cttcgtcaac
     4981 ctcaccacgt cgttgggaag cttcccggtc gccagcatct ccaccgtgac ctgttcgaaa
     5041 acgtcacgca ggctcgctcg ggcggcgatc cagaccagct ggagcaccgt cgcggactcg
     5101 ttgtaggtca ccgcctccgg ccgcagcccg tacacgctca ccagcgggcc gtcgaccgca
     5161 cggatgatgt cggcgacgct gacctcgtca ctgccgcggg ccagccgcca cccaccactt
     5221 tgtccgcgga cgctcatcac gatgcccgcg cgacgcaggt cggcaaggat cgcctgcagg
     5281 aatccgtgcg ggatgtcttg caacctcccg agctcctctg ccgacaccgg tccgccgtcg
     5341 ccacgggtgg cgatctcgat cagcgcccgc agcgcatagt cggacttggc ggagactcgc
     5401 atggggccaa gtttgacaga aggcgctcag gttggaccgt cgtcgcaacg cggagcagtg
     5461 tgtccgctgt gcgtgtcgag ccggacccgc aacatctgtg cacgaccaga ccgatccacg
     5521 tccgtggttc ccggtcgcgg ctgttccgcc acgcagtccc gggggggcgg gtggcaggct
     5581 gggcggtcgt gatcactgtc gacgcagaca cgatccggct cttcctccat gtcctcgcgg
     5641 cgacggtgtg ggtcggggga cagatcgtcc tcgccgcctt gctgcccacg ctgcgagcgg
     5701 ccggggcagc cgttcccggt gccgcggcac gtgcgttcaa ccgggttgcc tggccggcgt
     5761 tcggcgtcct cgtcctcacc ggtgtgtgga acgtcgtcgc cgagggcgac cggggtccgg
     5821 cgtacgagca caccttgatg ctgaaatacg tgctggtgct cgggtccggt gtgaccgcgt
     5881 tcgtgcacat gaaggcgagg tcccgagcga tgatggcggt cttcggcgcg ttgacgggac
     5941 tcttcgcact ggccgcactg ttcgtcggca tcatgctcgc cggctgagat gcactgtttg
     6001 cggcatccgg ttgatcgacg tgtcgatcgc cgtccgaaca ccaacacagg ccgcgtcgga
     6061 cgaacctgag cacctcgaaa tgcaggggag aagccgtacc aggaaccccg caaagcgttg
     6121 cggtgctgct cgcgaacgca cgacggccga gcacgaactc ccgatcagga gcgcacgacg
     6181 gtgtccgcga actcgaccaa cgtcccagcc gtccgcagac atgtcttgag attggcgccc
     6241 gaaacgttga tgactccgta gtgagcactg tccttcaagt ccagaaggcg gtcgaggttc
     6301 tttgctgcgt tcgccccgcc cggctcgacc tgtcgtacga gttcaaccgc ggccttgtgg
     6361 ccctttgccc gtgatcgacg gcccaaggat gcgcagcagg cagcatccac ggcggcgatg
     6421 ccagccaaga cggccgaggc tgcggcgaca ctggcggagg gcgccaccga gtcggcttct
     6481 gcgccgacca tcatcgccac ctcttggaac ttgcgcgcct ggtcaaggcg cgctaacgcc
     6541 tcggtgcgac cgcatggcgc ggttcgtccc tgcgaagcgg gcttcattcg aactcctcag
     6601 ctcccaggtc gaggacatcg gtcacggtga gtgcgacctt gaccttgcgg agttcttgga
     6661 caatgcgttc cttgcggtcc agcatgtcgg tcatctctgc gggggtgagc tggatgatgc
     6721 tcgccgcgtt tcccgtccac cggcggacgc tttgggccaa cgcatcgacc tgaccgctcc
     6781 agttcgggtc gtcgtcgcga gtgtcagccg gccggatgac gagaaggtct acgtcggaat
     6841 caactccgcc gtcgccgcgg gcggcggatc cgaagacagc cgccgcgacg ggtggcggat
     6901 cccaggagtt catggctttc cggatacgcg cgaaggcctc gcctcgcagg tcctgtagcg
     6961 ccaacaccgc tggagtggcc acatggtcgt ggttcagggt gtagaggttt gacattcctg
     7021 cagatgagac cgtgacgaga ccttggtcga ccatgcggtt gaggacgttg ctgacgcccg
     7081 ccgggctacc gcgccgagcg agacgagcga catcacggcc ggtgagtggt ctcgtcgtgc
     7141 tcgcaagcac atcgagaacg gcaccgtcga gcgagggcac gactgccgcg gccggctctg
     7201 accagtccat ccgctcttct ccagggtcta gtgaacagaa tactaggcga gtgtctcaga
     7261 tagttgccac gtgcccagta tttcggacgc aggggcctgg gctgggcggc ggagaatgtg
     7321 cctgtccagc cctctacggg gtgtgggcca cgatccggcg aatgcttgat tcggtctact
     7381 ggtgggtagc atactgaaag ttactcgcgg gtagtatgct cgcggcggct gcgtggccgg
     7441 tggaaggggt tcgatgaccc attacaagag caatctgcgc gacctcgagt tcacgttgtt
     7501 cgaggtcttc ggacgcgaca gcgtgctcgg caccggaccc ttcgccgacg ttgacggtgc
     7561 aacggcccgc gagatcctgc acgaggtcga tcggctggca cgcgaggagc tggccgagtc
     7621 gttcgcgagc gccgaccggc agccgcccgt gttcgacccc gacactcacg ccgtgacgat
     7681 gcccgaggat ctcaagcggg cgttcgcgtc atacatggac gcggagtggt accgcctcga
     7741 gctcccccgc gaactcggcg gccagcccat cccagcgagt ctgcgctggg cggtggccga
     7801 gctcgtcctc ggcgccaacc ccgcgatcca cctgtactcc gccggcccag cgttcgccgg
     7861 cgtcctctgg cgcaacggca ccgagcgcga ccggaggatc gcgcgccaca tggtcgaccg
     7921 tcgctggggg gcgacgatgg tgctcaccga gccggacgcc ggttcggacg tgggggcggc
     7981 tcgggcgaag gccgtcccgc tgccggacgg ccgctggcgc atcgaaggca ccaagcgttt
     8041 catcacctcc gccgagcacg acatggccga caacatcatc catctcgtgc tcgctcgtcc
     8101 cgtcggggtc gaaggggcgg gaggtccggg caccaaggga ctctccttgt tcatcgtccc
     8161 gaagcacgac ttcgacctcg agaccggcga gctgactgga cgacgcaacg gcgtccacgt
     8221 gaccggcgtc gagcacaaga tgggcatcaa ggcctccgcc acgtgcgagc tcaccttcgg
     8281 tgccgaggag ccggcgtacg gttggctgct cggtgaggtc cacgacggca tcgcacagat
     8341 gttccaggtg atcgagaacg cccggatgat ggtcggcacg aaggcgatgg cgaccctttc
     8401 gaccggctat ctcaacgcgc tggagtacgc caaggcacgc atccagggcc ccgacctgac
     8461 tcgctccgcc gacaagaccg cgccacgcgt cccgatcacg caccacccgg acgtacgccg
     8521 ctcgttgctc acccagaagg ctttctcgga ggcgcttcgt gcgctggtga tctacaccgc
     8581 gacataccag gacacggcag cattggcgcg agccaacggc cagaccgacg agctcgccga
     8641 gtccgtcaac gacctgctgc tgcccatcgt gaagggctac tgctccgagc gcgcctgggt
     8701 cgtcctgggc accgagtcgc tccagacgct ggggggttcc gggttcctgg aggactatcc
     8761 gatcgagcag tacgtgcgcg actcgaagat cgacacgctg tacgaaggga cgaccgccat
     8821 ccagggtatg gacctgttct tccggaagat cgtgaagaac caggggcggg ccctccgggc
     8881 gctggccgcc gagatcaccg gcttcctgca gagcgaggcc ggcaacgggc ggctcaagga
     8941 cgagcgcgag ctgctggcga acggcctggc ggacgcggag gcgctggtcg ggtcgctggt
     9001 cggccacctc atgtcgagca gccccgacgt cgaaggcggt gacatcacga acgtctacaa
     9061 ggcggggctg aacaccacgc ggctgttgat ggcactcggc gacgtcatct gcggctacct
     9121 gctgctcctg caggccgagg tcgccctcgg ccggctcgcc ggcgaggtct cgccgcggga
     9181 cgagctcttc tacgagggaa aagtggccgc cgcgcagttc ttcgcccgaa cagttctgcc
     9241 gaaggtgaca gcagaacgcg ccatcgccga gtcgacggac ctgatgccga tggagctgga
     9301 cgaagccgcc ttctgacgac gtctgcggcg ccacctgctg acacgtcagg acacggttcg
     9361 atcagggagc ggccggctcc cggatgccac acttgtccgc atggcgacgc cgccggtcga
     9421 ggaggaaacc atccggcccc gggccgcgtg gtacgtcgtc ccggccgcac tgtggctgac
     9481 cgccctgacg ctgttcgtca tcgcgctgct ctccatcgcg cacgtcgtca agtccggggt
     9541 cgacccgatc gacaaccacg cgttggtgga catcgacacg gacggtctca ccttctacac
     9601 gactggctcg cagtcgaagg tcacctgcgt gctcacacca ccgaatggca gcgccttctc
     9661 actggacctg ttggacgtca cgctgaacat cacggtggac gagaactcct accatgcgat
     9721 cggtgtcacc cccgacgacc taccggcagg ccggtaccgc ttcgactgca cgcctgtctt
     9781 gccgcggacg accttcggca ccggaccgcg cgtggacatc gcggcgctcg ccacccgggc
     9841 cttgtggggg atcttcctgc cgctggtgct cggtgtgatc ggcctggtcg tgctgatcgt
     9901 ggtcatcgtg aggcggcatg gcgcgaagat ccgcctcagg cagccgacac ctcctccccc
     9961 gccgccgtac tgacttgtca gaaataggtg gacctatagg tcgatggggt tctgacaggt
    10021 cggcgggcgg tttccacagg accgcgactg cgggtttcgt gcgggacgtt ctgtccgcca
    10081 cggtggcgag tcatgtacgt cgcacagtca tgcgtcccag gactttcctt gttggcgaca
    10141 taccagcacg gcgtggtcac ccgtcggcag ctgactggtc tcagcatgac accgggtcag
    10201 atcgacggcc agctcgatgc gagacgatgg cgggcggaga ctccgcgcgt ggtcgtgatg
    10261 cacaaccatc agctgacccg ggcacagttg atgtgggtgg cacttctgac ggcgggaagc
    10321 cgcgccgcgc tctgcagcca tacggcgctg gagtgcgccg gcttcagagc gttcgcctcc
    10381 gaagccgagc atgtgcatct gctcgtcccg aggggcgcga agaccttgga cctgccgaac
    10441 gtcaccgtgc acgaatcgag gcgcttcaag gactgggagc tcgtgtggtt caacggcctc
    10501 ccctgcaccg acacggcgcg gtcggcggtg gatgcggccg cgtggcagcg ttggccgcgg
    10561 ttcgcgatga ccatgcttgc cgccgtggtg cagcaacgct tgtgcacagc agcccagctc
    10621 gaccaggcac tcgcgcgcgt cggacgggtg cggcacaagc agtacatgcg gttggcgttg
    10681 cgcgacatcg caggcggcgc ggaggccctg ggcgagatcg atgtcgccag gatgtgccgg
    10741 cgattcgatc tcaagccgcc ggatcggcag gtggtcagac gagatccaag cggccgcaag
    10801 cgctacctcg actgtgagtg gaacctcggg aacggcgtga tcgtggtgct cgaagtcgac
    10861 ggctcacatc acttgaccgt cgagcactgg gaggcagaca tccgacgtga acgcaagatc
    10921 gtcacgtccg gcagactcgt gctccgggcg acctcggctg agctgcgtct cgatccccac
    10981 gaggtggcaa ccgacctgcg cgccctcggc gtaccgagtt gtcagaagta ggtggaccta
    11041 taggtcgatg gggttctgac aagtcggtca gaggtggcgg cgggactgca cgaagcggaa
    11101 gcggctggcg acgaaggccc cgtcggtgta cgtggcgttg gatgcgggat tggccccggt
    11161 cccgtggaag tcgctgaacg ccgcggactg gttgacgaag acctggccgg tgaggttctc
    11221 gctgagcgcg acgccggcgt cgagggcggc cgagcgcatg gcggccacga cgtcgtcgtc
    11281 ggtcgagtac accgaggctg tcatcgcgcc gtggtcgatg acggtacgcc ggaacgtgtc
    11341 gatcgactca gcggtcgacc cggtccggat caggtagccc accggcccga aacactcgga
    11401 ctcgtaggtc ttcgcatcgc tcgcgtccag cgccacgagc gtcggcgtac gaaccacggc
    11461 gtcctcgaac gtcggatggt tcacctcgcg cgactcgatc aggacggttc cggccgaccc
    11521 ggcagcggcg agccgttcga ggacgccgtc gttgacgacg ccgcccaaca cctcgacggc
    11581 tcgggcgtcg tcaccgacaa gcttgcccag cgccccttcg aggcccgaga cgacgtcgtc
    11641 gacgctcttg tgtccctcgt cggtgtcgat gccctccccc ggaatgaaga ggttctgtgg
    11701 agcagtgcac atctgaccgg tgtagagcgc cagcgagaat gcgaggttga agcacatgcc
    11761 cttgaacgag tccgtcgagt cgatcacgac cgtgttgacg ccggacttct cggtgaagac
    11821 gatggcctgc cgggcgttct cctccagcca ctccccgaac gcgctgccgc cggtgaagtc
    11881 gacgagcttc acctccggcc gaaccgccag cactgctgcg atgccgtcgg ccgggtcctc
    11941 cgctgccaag gtgacgatgt tcgcgtcgta ccctgcctcg gccagcacct cctgacacac
    12001 ctgcaccgtg atcgcgagcg gaagcaccgc ccccgggtgc ggcttcacga tcacgggatt
    12061 gccggtgacc aacgacgcga acaggccggg ccacgagttc cacgtgggga acgtgttgca
    12121 gccgacgacc agcgcgagac cgcgcggcac gaccgtgaac gtcttctcca tccgcagcgg
    12181 cgcatcgcgt ccgggcttct cccagacagc agtacctgga acgcgcgtca tctcgatgta
    12241 cgcgtacgcg atcgcctcga gcgcgcggtc gagggcatgg gcgccacccg cctggaacgc
    12301 catcacgaag gcctggcctg atgtgtgctg aacagcgctg gcgagctcga acacccttgc
    12361 atgcaagcgt ttcaggactt ccagaccgac accgacgcgg gcttcgatcc cgcaatcacg
    12421 ccaggcggtc agcccggcgg acgccgcctc gaccagcgcc ggtacagcgt ccggcgtcac
    12481 ccgtgggtag cgcacgccga gcggcttgcc gaacggcgac ttctccgtcg ccaccgtccc
    12541 cgcagcaccg ggcacag
//
LOCUS       DATBFZ010000515         2826 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_4513329, whole genome shotgun sequence.
ACCESSION   DATBFZ010000515 DATBFZ010000000
VERSION     DATBFZ010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2826
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4513329"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..600)
                     /locus_tag="VH419_15890"
     CDS             complement(<1..600)
                     /locus_tag="VH419_15890"
                     /inference="COORDINATES: protein motif:HMM:NF012344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEY7045152.1"
                     /translation="MSLTACSQDDVDQLKRLGLPEPGSDRAVYIHDLWIGSWIAAGII
                     GGLTWFLILWSAIHYRRRSTTDLPTQVRYNLPIEVLYTIAPIIVVSVLFFFTVEKQNK
                     VLEAVDNPDHRVVVTGQQWSWTFNYLNEPGAGGEDVYDAGTSTIEPTLWLVEDQTVTF
                     TLHSPDVIHSFWVPSFYFKRDVIPGRDSSFSITPTAAGDF"
     gene            complement(872..1861)
                     /locus_tag="VH419_15895"
     CDS             complement(872..1861)
                     /locus_tag="VH419_15895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HEY7045153.1"
                     /translation="MPIAVTGSVATDHLMSFPGRFADSLVVDQLDKIALSFLVDDLQV
                     RRGGCAANIAFGLAALGVRPVLVAAVGEDFADYRSWLERHGVDCAALHYSQTRHTARF
                     ICTTDTSHAQIASFYAGAMSEAREIELKPIMDRVGGLDLVVIAPNDPEAMIRHTHECR
                     DRDIPFAADPSQQLAFSDGETIRDLVDGAEILLSNEYESALMEQKTGWTSAEILKRVG
                     TRVVTLGQAGVRVDSVDADPIVVPAVPDVTAVEPTGVGDAFRSGFLAAVSAGLSWRSA
                     AQVGCVLAAYVVERVGTQEYSFSHQSFVNRLARAYGDDAAADVAGWLAPGTSS"
     gene            1939..2175
                     /locus_tag="VH419_15900"
     CDS             1939..2175
                     /locus_tag="VH419_15900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7045154.1"
                     /translation="MSAPTPRRHRDAEHVALPYTESSTLDEAYDDATQVARAYPVVRL
                     PHQDIVNKMVGDARWNEICEEISTHGALERPYLD"
     gene            complement(2282..2641)
                     /gene="erpA"
                     /locus_tag="VH419_15905"
     CDS             complement(2282..2641)
                     /gene="erpA"
                     /locus_tag="VH419_15905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HEY7045155.1"
                     /translation="MTVQDEATTTAVDGVLLTDNAATKVRTLLDQEGRDDLQLRVSVQ
                     PGGCSGLRYQLFFDERHLDGDTVKDFSGVAVVVDRMSTPYLHGATIDFVDTIEKQGFT
                     IDNPNATGSCACGDSFH"
BASE COUNT          467 a          944 c          945 g          470 t
ORIGIN      
        1 gaagtcaccg gccgctgtcg gtgtgatcga gaaggaggag tcgcgcccgg ggatcacatc
       61 ccgcttgaag tagaacgacg gcacccagaa cgagtggatc acgtccggcg agtgcagcgt
      121 gaacgtgacc gtctggtcct ccaccagcca caacgtcggt tcgatggtcg aggtgccggc
      181 gtcgtacacg tcttcgcccc cggcgcccgg ctcgttgagg tagttgaacg tccacgacca
      241 ctgttgcccg gtcaccacga cccggtggtc cgggttgtcg accgcctcga gcaccttgtt
      301 ctgcttctcg acggtgaaga agaagagcac ggagacgacg atgatcggcg cgatcgtgta
      361 caggacctcg atcgggaggt tgtagcggac ctgcgtcggg aggtctgtcg tgctgcgccg
      421 ccggtagtgg atggccgacc acaggatcag gaaccacgtg aggccgccga tgatgccggc
      481 ggcgatccag gaaccgatcc agaggtcgtg gatgtagacc gcccggtcgc tgcccggctc
      541 cggcaggccg agccgcttca gctggtcgac gtcgtcttgc gagcacgccg tcagggacac
      601 caggagaagg acaccaacgg ccgccgttgc cacccgacgg gtcagcggtc gactacgtcg
      661 cccgcggcca cattcgctca gatgtcgact cacgtggcgc cttcctcgtt ggtcgaatcg
      721 gtgacgttcc cgtgacgcat caggtgtcga atcgcgctgg tggggggcca ggtcggttca
      781 gcagaaccct actccagcag actggtcagt tcgctcgcag gtggaaggtg ttgtccgcct
      841 gatcgtccaa cggcagcggt gctgtttgct gtcagctgga ggtccctggg gcgagccacc
      901 cggccacgtc cgctgccgcg tcgtcgccgt atgcgcgggc cagccggttc acgaaggact
      961 gatggctgaa ggagtactcc tgagtgccga cacgttcgac gacgtacgcc gccagcacac
     1021 agccgacctg ggccgccgat ctccaggaca gcccggccga gaccgcggcg aggaacccgg
     1081 agcggaacgc gtcgccgaca ccggtgggct cgacggcggt cacgtccggc accgccggta
     1141 cgacgatcgg gtcggcgtcc acgctgtcca cgcggacccc cgcctgcccc agcgtgacga
     1201 ccctggtgcc gacccgcttg aggatctccg cggaggtcca gccggtcttc tgctccatca
     1261 gggcggattc gtactcgttg gagagcagga tctcggcacc gtcgaccagg tcacggatcg
     1321 tctcgccgtc ggagaaggcc agctgctgcg acgggtccgc ggcgaacggt atgtcgcggt
     1381 cccggcattc gtgcgtgtgc cggatcatcg cctccgggtc gttcggggcg atgaccacga
     1441 ggtccaggcc gccgacgcgg tccatgatcg gcttcagctc gatctcccgt gcctcgctca
     1501 tcgctcctgc gtagaacgag gcgatctggg catgagacgt gtccgtcgta cagatgaagc
     1561 gggccgtgtg gcgcgtctgc gaatagtgca gtgcagcaca gtccacgccg tgccgttcga
     1621 gccacgagcg gtagtcggcg aagtcctcac ccacggcggc gaccaggacc ggccgtacac
     1681 ccagtgcggc caggccgaac gcgatgttgg ccgcgcaacc gccccggcga acctggagat
     1741 cgtcgaccag gaaggacagc gcgatcttgt cgagctgatc cacaacgagg gagtcggcga
     1801 accttcccgg gaacgacatg aggtgatcgg tcgcgaccga tccggtgacg gcgatgggca
     1861 cggccacaca ctacccatgg gtacgccacg ccaggcggac ccttgcatgg agttccttca
     1921 gcggcctacc gttgccctgt gagtgcgccg accccacgca ggcaccgcga cgccgagcac
     1981 gtcgccctgc cgtacaccga gtcgtcgacg cttgatgagg cgtacgacga tgcgactcag
     2041 gtggcgcgcg cctaccccgt cgtacgcctc cctcaccaag acatcgtgaa caagatggtg
     2101 ggcgacgccc gctggaacga gatctgcgaa gagatctcga cgcacggcgc cctggaacgc
     2161 ccctacctcg actgaagccg accccggcca cgccagctcc ccgcgaattg cggggagttc
     2221 cgcgttttct tccctcggaa tcccggaacc tcccgcattt cgcgggatcg gtggcgcggg
     2281 atcagtggaa ggagtcgccg caggcgcagg agccggtggc gttggggttg tcgatcgtga
     2341 agccctgctt ctcgatcgtg tcgacgaagt cgatcgtcgc accgtgcagg tagggcgtgc
     2401 tcatccggtc cacgaccaca gccacgccgc tgaagtcctt gaccgtgtcg ccgtcgaggt
     2461 gccgctcgtc gaagaacagc tggtagcgca gaccggagca gccgccgggc tgcacggaga
     2521 cgcgcagctg caggtcgtca cgaccttcct ggtcgagcag tgtcctgacc ttcgtcgcag
     2581 cgttgtcggt caggagcacg ccatcgacgg cggtcgtggt ggcttcgtcc tggacggtca
     2641 ttcgtgtgct ccccaaggtt cggtccgctt gccatcccca tgataacggt tcaacggaca
     2701 actcctgcac cagcgcgaag ttctggtctc gcccctcgcc cccaagcgtg gcccggtgcc
     2761 gtcgttggcc gcggtgggcc gcggccgcgc gtgctaccgg tgcattcctc acgcgtctgg
     2821 gcccgc
//
LOCUS       DATBFZ010000516        13083 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_4514327, whole genome shotgun sequence.
ACCESSION   DATBFZ010000516 DATBFZ010000000
VERSION     DATBFZ010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 13083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13083
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4514327"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="VH419_15910"
     CDS             complement(<1..399)
                     /locus_tag="VH419_15910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017933355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEY7045156.1"
                     /translation="MARVLVVDHHDSYTYNLVHLIAGVTGTLPDVVQHDDPEAARCWS
                     DDYTHVVLSPGPGTVHDERDFALGRTIVATARMPVLGVCLGMQGIVEGSGGGVEVVDP
                     AHGEVSRVMHTGSGVFSGLPQGFAAVRYHSQ"
     gene            complement(414..2285)
                     /locus_tag="VH419_15915"
     CDS             complement(414..2285)
                     /locus_tag="VH419_15915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007079381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase family protein"
                     /protein_id="HEY7045157.1"
                     /translation="MSLVDDVVSAPAYALVRLHGDDHVTLLTGRPEIHAELAEVPLDD
                     GRPEAPRLDRLVCLPFSQIRERGFRARMDDAQLVSIEIEHQSRTPIEALVGQLPDEPI
                     STVGEPGFDIDDAAYAEIVRTVIDSEIGNGEGANLVIARNFTATIADWSHLKALTVFR
                     RLLERERGAYWTFVVFTGDRFLIGASPERHISVRSGEVRMNPISGTFRVGSAVDAGSR
                     KQRLMDFLADEKEIYELFMVVDEELKMMCDICSEGGQILGPYLKPMTHLIHTEYILAG
                     RTHKDVRDVLRSSMYAATVTGSPVENACRLIEKYEQQGRGYYGAVLALLGRDSDGGPT
                     VDAPIVLRTADVRPDGRLRVTAGATLVRDSQPAYEVAETHAKAGGILTAFGLAEPPAT
                     PATEIADLAQDEDVLVALGRRNQRLSRFWFNDQSDAERASALSDRHVVVLDGEDDFVT
                     MLRHMLAVLGMTSETVRHHDYRQDSFDGADLVILGPGPGDPRERDDPKIKAVRAAVDD
                     LLARDIPFLAVCLGHQVLSSALGLELAYKDIVFQGTQTRIDLHGSQENVGFYNTFVAK
                     QPANPKEGLDIQADPKTGDVHLLAGRGFRGIQFHAESILTENGFDLVRRLLVELLDL"
     gene            complement(2387..4558)
                     /locus_tag="VH419_15920"
     CDS             complement(2387..4558)
                     /locus_tag="VH419_15920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012920481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEY7045158.1"
                     /translation="MPNRARNLEPSETGHVSTHVVEQEIKHEQAVVDRIYGRLEAATQ
                     AARALAEEGHARARLGTDSGLVERDAIVFQASRRLATLNAAHEGLVFGRLDMRADGPR
                     YVGRLGLRDEDREVLLIDWRAPAASVFYQATAQDPLGVVRRRVLQSSGDTVIGIEDDL
                     LDPEHAPDDLVVVGEGALIASLSRARDRAMHSVVATIQKEQDDAIRAPQKGVTTISGG
                     PGTGKTVVALHRAAYLLYTDRRRFEGGGVLVVGPNAVFMHYIERVLPSLGETSVSLRA
                     LGEVVDGIVAARHDLPTVAAIKGSSRMKTLLARTARGPVPDAPTSFRFFYRDDVLTLT
                     EPELRALRRTLLGSGQRRNRVAPKVADELTELLWTKAAGDRAREHGHDEFAHTLRESR
                     EFNDFVRAWWPVVDAVDVYGWLVDRDRLSRDAHALLNKVEIDELLESFGSDFTVEDVP
                     LIDELRYLLGEVPEPEPDEDPLADLYDETVPELSTFDERQWGRSVRWRSGQRPTASIE
                     DDLYAHVLVDEAQDLSPMQWRMVGRRGRYASWTIVGDPAQSSWSRPDEAATARADALR
                     GKDQNAFRLSTNYRNSREIFELAADVARTWIPGADLPDAVRETGVAPRIHDVEPAALA
                     LAVREAMERLVDEVEGTIAVVAPVGRRDEVEGWLGDRDAERIPVLDAIDTKGLEFDGI
                     VVVQPDEIVAESEVGIRTFYVVLTRATLELDVIGTSHHWRP"
     gene            complement(4733..5194)
                     /locus_tag="VH419_15925"
     CDS             complement(4733..5194)
                     /locus_tag="VH419_15925"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY7045159.1"
                     /translation="MIRPATSDDLLTLVEMERQASTTGLSHIFGPDLPFPAADVLARW
                     RIVIDDADVVTLIDEDESGPVGYAAFGGGWLRHFGYLPRAWGTGRPHVLHAEALAGLA
                     AGGAQPAYLWVLVENHRARAFYARRGWQDTGVREREVFEPYPEKMQLMLRT"
     gene            5258..6694
                     /locus_tag="VH419_15930"
     CDS             5258..6694
                     /locus_tag="VH419_15930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011753620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY7045160.1"
                     /translation="MAEGIRYKSTTGRWVLAAAVLGSGMAFLDGTVVNVALRTIGRDL
                     DASLSQLQWITNGYMVSLASLILLGGSLGDHFGRRRVFVVGVVWFAAASLVCGLAQSP
                     EQLIVARVLQGVGAALLTPGSLAMIQGSFHPADRARAIGAWSGLGGIAAALGPFVGGW
                     LVQYATWRLVFLINLPLAAFTVILAQRHVPETKDPDSVPGFDSVGAALGAVGLAGVTF
                     ALIEADSMSIPAVVASAVVGVVAFTAFIQVERTSRHPMMPTGLFASRQFSAANAMTLL
                     VYAALGGVAFFLVLELQTVSGYGPLGAGISMLPVTIVMLFGAPRGGQLAQKIGPRIPM
                     TVGPLLCAAGIALLTGVGADASYWVDVLPGVTIFAIGLALLVAPLTATVLAAAPDRFA
                     GVASGINNAVARTGSLLAVAALPAAVGLSGADYDDPTAFNEGFRMAFGICAGLLAAGG
                     VVSWLLIRNPLPAGDGRVTAAEHQGPDH"
     gene            complement(6731..6928)
                     /locus_tag="VH419_15935"
     CDS             complement(6731..6928)
                     /locus_tag="VH419_15935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020392668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7045161.1"
                     /translation="MTPDVPEYCDRCGGALSDTDHSACRRERELEPPRYCGFCRRRMV
                     VQVLPTGWVARCVEHGLVTSE"
     gene            complement(6925..7929)
                     /gene="bioB"
                     /locus_tag="VH419_15940"
     CDS             complement(6925..7929)
                     /gene="bioB"
                     /locus_tag="VH419_15940"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020392667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HEY7045162.1"
                     /translation="MTVDVDVLGVARLQALDEGRGLSEAQLVEVLRLPDDALPALLAL
                     AHQVRMRWCGPDVEVEGIVSVKTGGCPEDCHFCSQSGKFESPVRAAWLDIPSLVGAAK
                     QTAATGATEFCIVAAVRGPDERLLRQVEDGITAIRTEVDINIACSLGMLTPEQVDRLA
                     AMGVHRYNHNLEAARSYFPHVVTTHSFDERWETCLLVREAGMELCCGGLVGMGESLEQ
                     RAELASQLAELDPDEVPLNFLNPRPGTPFGMLPVMGSADALRTVAAFRLAMPRTVLRY
                     AGGREITLGELGTRDGLLGGVNAVIVGNYLTTLGRDPAKDLDLLDELQMPIKELSKTL
                     "
     gene            8109..9389
                     /locus_tag="VH419_15945"
     CDS             8109..9389
                     /locus_tag="VH419_15945"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009074845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HEY7045163.1"
                     /translation="MLQSAVDERLAFDREHLWHPYSSMTSPLPARYVVSAEGVRLRLS
                     DGTELIDAMSSWWCAIHGYRHPHLDDAVRRQLDSMSHVMFGGLTHEPAIELARRLIGL
                     SPEPLQHVFFADSGSVSVEVALKMAVQYQRGRSRPRRTRMLALRGGYHGDTTGAMSVT
                     DPVGGMHSMFADVLPQQVFADRPPAPGSDIAAWSSHVRSLAAAHADDIAAVFCEPVLQ
                     GAGGMYVYEPACLNVLREICDEHGFLLVLDEIATGFGRTGTLFAAEQAGIAPDIMCVG
                     KALTGGYLTLAATLCTTDVAAGVGSSEAAALMHGPTFMANPLACAAASASLDLLDERD
                     WRADVHRIESRLRQYLEPARHSAQVVDVRVLGATGVIQLAHQVDVVRASATAAKHGVW
                     LRPFRDLVYTMPPYVTSDGELDQVCTALIAAASA"
     gene            9386..10513
                     /locus_tag="VH419_15950"
     CDS             9386..10513
                     /locus_tag="VH419_15950"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012923569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HEY7045164.1"
                     /translation="MSLDDWLVRRRSRREEAGLQRFLHTRPATSDVIDLAGNDYLGLS
                     RDPRVTAAAAEAASRWGAGAGASRLVTGTHEIHNRLEHALADFTGHAAALVFSTGYHA
                     NLGVVSALADADTLLVSDAHVHASLVDACRLARAGGLTITPHNDLTAVEEVLRTRSHD
                     RALVLVESVYSVLGDAAPLVELAGLVDAYDAVLLVDEAHAVGVAGKDGSGLVATCGLA
                     GRPDVVTTMTLSKALGSQGGAVLGSTALVDHLVNTARPFIFDTGLAPAAAGGALAALG
                     VLRDEPGLPHDVLATACRLADAAGVDQPAAAVLSVPMGSPQEALSAQHWLAGEGVQVG
                     CFRPPSVPDGMSRLRVTARADLAPAEVALACRLLRHIERRR"
     gene            10510..11226
                     /gene="bioD"
                     /locus_tag="VH419_15955"
     CDS             10510..11226
                     /gene="bioD"
                     /locus_tag="VH419_15955"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012923568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HEY7045165.1"
                     /translation="MRFVVVTGTGTGVGKTVATAAFVAAALEQRRRVCVVKPYQTGVT
                     SDEPSDVASVVALTGCLDVHELVRLDDPLAPETAARLRAIEIPTVQAIADEVVSCTRQ
                     ADVTFVEGAGGVAVRLDTEGGTLISLAAALRSYGHDVDVVVVTGLGLGTLNHTALTVM
                     ALRAEGFEPHGLVFGDVVEPLGLAERCNVTDLPRITQVPVVASIPHGVGRLRREEFLA
                     VASSWMLDGRLFDRSETDQR"
     gene            complement(11217..>13083)
                     /locus_tag="VH419_15960"
     CDS             complement(11217..>13083)
                     /locus_tag="VH419_15960"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEY7045166.1"
                     /translation="LVLTACTGGSDEPSPTESPDTADSGGAIINEFATAWADPSVSAF
                     RGLVDEPGVAAHDIAAHVAELGIIRTTVEPSGDLDCDSDSCKEHARVTHELAGAGEWS
                     YETLIRADLNQGQWLVSWSPSTFHPDLTDVTTLVRHRSLPPRAPILDRNGVALTPERS
                     IVRVGVVAGKVRPATYDDLSQLLDIDPSALRDRVAASKPGWFVSVIDLRRADYEPLRD
                     DLLQIPGISIDTAKRALAPTSAWGRAVLGTVGPATEDTLKNAGPLALPTDEVGTSGLQ
                     YAFQQRLAGKPGITIDLVEKAPGGSTLDQVFVRRARTGEPLETTLDLRAQNAAEKAVE
                     EATDTTAVVLVKASTGEILAAANAPGPTSYNTAFVGQYAPGSTFKTVSSAALLDAGVV
                     TPQTRVECPDSTVVGGKRFRNYEQGITGPNPTFAQAFAASCNTTMVSFADEITGQQLA
                     AEGTAFGFGQKWDLGIDSYSGSVPADSDLVTRAADMIGQGKVLASPLMMAMTAAAVDS
                     GVSRTPTLLPGELPGKRLEELNPDTIASLQQMTRLVVTRGTGTSVNLPGLPVHAKTGT
                     AEYANGSVTGTNAWMIGYRGDVAFAVFVANGTSGAHDAAPIVADVLAELPASLYR"
BASE COUNT         1925 a         4580 c         4458 g         2120 t
ORIGIN      
        1 ctgtgagtgg taacggacgg ccgcgaaccc ctgcggcagg ccgctgaaga ctcccgaccc
       61 ggtgtgcatg acgcgcgaga cctctccgtg cgcgggatcg accacctcga ctccaccgcc
      121 ggatccttcg acgatcccct gcatgccgag gcacacgccg agcacgggca tcctggcggt
      181 ggccacgatc gtgcgcccga gcgcgaagtc gcgttcgtcg tgcacggttc ccggtcccgg
      241 cgagagcacg acgtgcgtgt agtcgtccga ccagcagcgg gctgcctctg gatcgtcgtg
      301 ctgaaccacg tcgggcagtg tcccggtgac gccggcgatc aggtgaacca ggttgtaggt
      361 gtacgagtcg tggtggtcga cgacgagcac gcgcgccata ccgcagtcac tgctcagagg
      421 tccagcagct cgacgagcag ccgtcgtacg aggtcgaagc cgttctcggt caggatcgac
      481 tccgcgtgga actggatccc acggaaccct cgacccgcca ggaggtgcac gtcgccggtc
      541 ttggggtcgg cttggatgtc gagaccttcc ttcgggttgg ccggctgctt cgcaacgaac
      601 gtgttgtaga acccgacgtt ctcctggctg ccgtgcaggt cgatccgggt ctgcgtgccc
      661 tggaacacga tgtccttgta cgcgagctcg aggccgagcg cactcgacag cacctgatga
      721 cccagacaca ccgcgagaaa ggggatgtcg cgtgccagca ggtcgtcgac ggcggcgcgg
      781 accgccttga tcttcgggtc gtcccgctca cgcggatcgc ctggcccagg ccccaggatg
      841 acgaggtctg caccgtcgaa gctgtcctgg cggtagtcgt ggtggcgcac ggtctcgctc
      901 gtcatcccga ggacagcgag catgtgacgg agcatggtca cgaagtcgtc ctcgccgtcg
      961 agcacgacca cgtgccggtc actcagcgcg gatgcgcgtt cggcgtcgga ctgatcgttg
     1021 aaccagaacc ggctgagtcg ctggttgcgc ctgccgagtg cgaccaggac gtcctcgtcc
     1081 tgggcgaggt cggcgatctc cgttgcgggg gtggcaggcg gctctgccag cccgaacgcc
     1141 gtcaggatcc cgccggcctt cgcgtgggtt tccgccacct cgtacgccgg ttgggagtcg
     1201 cggaccaagg tcgcgccggc agtgacccgt agccgcccgt ccggccggac gtcggccgtc
     1261 cgcaacacga tcggcgcgtc gacggtcgga cccccgtcgc tgtcgcgtcc gagcagcgcc
     1321 aacacggcgc cgtagtagcc ccggccctgc tgctcgtact tctcgatgag tcgacaggca
     1381 ttctccaccg ggctgccggt gacggtggcg gcgtacatcg aggagcgcag cacgtcgcgc
     1441 acatccttgt gtgtgcggcc ggccagaatg tactcggtgt ggatgaggtg cgtcatcggc
     1501 ttgaggtacg ggccgaggat ctggccgccc tcactgcaga tgtcgcacat catcttcagc
     1561 tcctcgtcga caaccatgaa gagctcgtag atctccttct cgtcggcgag gaagtccatc
     1621 agccgctgct tgcgagatcc cgcgtcgacc gccgacccga cacggaacgt gccactgatg
     1681 gggttcatcc tcacctcgcc cgagcgcaca ctgatgtggc gttccgggct cgcaccgatc
     1741 aggaagcggt cacccgtgaa gacgacgaac gtccagtagg caccccgctc acgctccagc
     1801 agccgtcgga acaccgtcag cgccttgagg tggctccagt cggcgatggt ggcggtgaag
     1861 ttgcgggcga tgacgaggtt ggcgccctcg ccgttgccga tctcggagtc gatgaccgtg
     1921 cggacgatct cggcgtacgc cgcgtcgtcg atgtcgaacc cgggctcgcc gaccgtgctg
     1981 atcggctcgt cgggcagctg tccgacgagc gcctcgatcg gcgttctcga ctgatgctcg
     2041 atctcgatgc tcaccagttg cgcgtcgtcc attctggccc ggaagccgcg ttcgcggatc
     2101 tgagagaagg gcaggcagac gagccggtcg aggcgaggcg cctcgggacg accgtcgtcc
     2161 agcgggacct cggcgagctc ggcgtggatc tccggtctcc cggtgaggag ggtcacgtgg
     2221 tcgtcgccgt gcagccggac cagcgcgtac gcgggtgcgc tgacaacgtc gtcgaccagg
     2281 ctcatgcgct cgaccccttc gggtgcggga cctgttctcc cgctcgggtg gtccgaggct
     2341 accaacgcca ccgcgcacgc aacggctgcc cgcgaccccg tgactgtcag ggacgccagt
     2401 ggtgactggt gccgatgacg tcgagctcga gagtggcgcg ggtgaggacc acgtagaacg
     2461 tgcggatgcc cacctccgac tcggccacga tctcgtccgg ctgcaccacg acgatgccgt
     2521 cgaactccag gcccttggtg tcgatggcat cgagcacggg gatgcgctcg gcatcgcgat
     2581 cgcccaacca gccctcgacc tcgtcacgtc ggccgaccgg cgcgaccacg gcgatggtgc
     2641 cctcgacctc gtcgaccagg cgttccatcg cctcccgaac cgccagcgca agggcggcgg
     2701 gttccacgtc gtggatacgc ggcgcgacgc cggtctcccg aaccgcgtcc ggcagatcgg
     2761 cgccggggat ccaggtgcgg gcaacgtctg cggccagctc gaagatctcc cgggagttgc
     2821 ggtagttcgt ggacagccgg aaagcgttct ggtccttgcc tctcaatgcg tcggcccgcg
     2881 ccgtcgccgc ctcgtccggt cgggaccacg acgactgggc tggatcgccg acgatcgtcc
     2941 agctcgcgta ccggccacgc cgaccgacca ttcgccactg catcggactc aggtcctgcg
     3001 cctcgtcgac gagcacgtgg gcgtagaggt cgtcctcgat ggaggcggtc ggccgctggc
     3061 ccgaccgcca gcggaccgat cggccccact gccgctcgtc gaacgtggac agctcgggaa
     3121 cggtctcgtc gtacaggtcc gccagcgggt cctcatcagg ctccggctca ggcacttcgc
     3181 ccaggaggta ccgcagctcg tcgatgagcg ggacatcctc gacggtgaag tcactcccga
     3241 aggactcgag cagctcgtcg atctcgacct tgttcagcaa cgcgtgggcg tcgcgtgaca
     3301 accggtcgcg gtcgaccagc cagccgtaca cgtcgacggc gtccaccacc ggccaccagg
     3361 cacggacgaa gtcgttgaac tcacgactct cccgcagtgt gtgcgcgaac tcgtcgtgcc
     3421 cgtgctcgcg ggcacggtcg ccggccgcct tcgtccacag cagctcggtc agctcgtcgg
     3481 caaccttcgg tgcgacgcgg ttccggcgct gcccggagcc gaggagggta cgccgcagcg
     3541 cccgcagctc cggctcggtc aacgtcagca cgtcgtcgcg atagaagaac cggaacgacg
     3601 tcggcgcgtc cggcaccgga ccccgggccg ttctcgccaa cagggtcttc atcctcgacg
     3661 agcccttgat cgccgccacc gtcggcaggt cgtgccgcgc ggccacgatc ccgtcgacca
     3721 cctctccgag ggcacgcagc gagacgctgg tctcgcccaa cgacggaaga acccgttcga
     3781 tgtagtgcat gaacacggcg ttcgggccga ccacgaggac gccgccgccc tcgaaccggc
     3841 ggcggtcggt gtagagcagg tacgccgcac gatgcagcgc gaccaccgtc ttgccggttc
     3901 ccggaccgcc actgatcgtg gtgacaccct tctgtggtgc ccggatcgcg tcgtcctgct
     3961 ccttctggat ggtggcgacc acggagtgca tggcccggtc acgggcgcgg ctcagcgacg
     4021 cgatcagggc gccctcgccg accaccacga ggtcgtccgg agcgtgctcc gggtcgagca
     4081 ggtcgtcctc gatcccgatg acggtgtcgc ccgaggactg gagcacccga cggcgtacga
     4141 cgcccagcgg gtcctgggcg gtggcctgat agaacaccga ggcggccggg gcacgccagt
     4201 cgatcagcag cacctcgcgg tcctcgtcgc gcaggccgag ccggccgacg taccgcggtc
     4261 cgtcggcacg catgtcgagc cgaccgaaga ccagcccctc gtgtgcggcg ttcaacgtgg
     4321 ccagccgcct gctggcctgg aagacgatgg cgtcacgctc gacgagcccg ctgtcggtgc
     4381 cgagacgcgc ccgggcgtga ccctcctcgg cgagcgcgcg agcggcctgg gtggcggcct
     4441 cgaggcgtcc gtagatgcgg tcgacgaccg cctgctcgtg cttgatctcc tgctcgacca
     4501 cgtgggtgct gacatgcccg gtttcggatg gctccaggtt gcgcgctcgg ttcggcacgg
     4561 gtgctcgttc ctatcccccg aaaatttgat gaacatacga tcttagacca cgccaccggg
     4621 ccatcgcggc ggatttccga cggcctcgac cggcgtacga acgccatcga ggtggcaatg
     4681 acgggccgac acgccgacag aaacgaacga aacctgccac ctcggcggga ggtcaggtgc
     4741 ggagcatcag ctgcatcttc tccgggtagg gctcgaagac ttcacgctcg cgtaccccgg
     4801 tgtcctgcca cccgcgccgt gcgtagaagg cgcgggcccg gtggttctcg accagcaccc
     4861 acaagtacgc cggctgcgca cctccggcgg cgagcccggc cagcgcctcg gcatggagca
     4921 cgtgcggacg acccgtgccc cacgcgcgtg gcaggtaacc gaaatggcgt agccagccgc
     4981 caccgaaggc ggcatagccc accggcccgc tctcgtcctc gtcgatcaac gtgaccacgt
     5041 cggcgtcgtc gatgacgatg cgccaccggg cgaggacgtc ggcggccggg aacggcaggt
     5101 cggggccgaa gatgtgggac agccccgtcg tcgaggcctg gcgttccatc tcgaccagcg
     5161 tcagcaggtc gtcgctggtc gccggacgga tcacgttcgc acgctaccca atcggcgtag
     5221 gacggttctc ccatccgagc gatggcaggc tggtgcgatg gccgaaggga tccggtacaa
     5281 gtccacgacc ggccgttggg tgctggcggc agcagtgctc gggtcgggaa tggccttcct
     5341 cgacgggacc gtggtcaacg tcgcgttgcg cacgatcggc cgagacctcg acgcgagcct
     5401 ttcgcagctg cagtggatca ccaacggcta catggtctcg ctggcgtcgc tgatcctcct
     5461 cggcggctcg ctcggcgacc acttcgggcg acggcgggtg ttcgtcgtcg gcgtcgtctg
     5521 gttcgccgcc gcctcgctgg tgtgtggtct cgcgcagagc ccggaacagc tcatcgtcgc
     5581 ccgtgtcctg caaggtgtcg gcgccgcgct gctcacgccg ggaagcctcg cgatgatcca
     5641 aggctcgttc cacccggcgg accgagcgcg tgcgatcggg gcgtggtccg gactcggcgg
     5701 gatcgcggcg gctctcgggc cgttcgtcgg cggttggctg gtccagtacg cgacctggcg
     5761 gctggtgttc ctgatcaacc tgccgctcgc agcgttcacg gtgatcctcg cgcagcggca
     5821 cgtcccggag acgaaggacc ctgactccgt gccggggttc gacagcgtgg gggcagcgct
     5881 cggcgccgtc gggctggccg gtgtcacctt cgcgctgatc gaggcggact ccatgtccat
     5941 ccctgcggtc gttgcatctg ccgtggtcgg ggtcgtggcc ttcacggcct tcatccaggt
     6001 cgaacgcacc agtcgacacc cgatgatgcc gacgggactg ttcgcctctc gacagttcag
     6061 cgcggcgaac gcgatgacgc tgctcgtgta cgccgccctg ggcggcgtgg ccttcttcct
     6121 cgtgctcgag ctgcagaccg tctccgggta cgggccgttg ggagccggga tctcgatgct
     6181 tcccgtcacg atcgtgatgc tgttcggggc gcctcgcggc ggtcagctgg cgcagaagat
     6241 cggaccacgc atcccgatga cggtcggtcc gctcctgtgt gcggccggga tcgcgctgct
     6301 caccggtgtc ggcgcggatg cgtcgtactg ggtcgacgtg ctccctgggg tcacgatctt
     6361 cgcgatcggg ctcgcgctgc tggtggcacc gctgaccgcg acagtgctgg ccgccgctcc
     6421 cgaccggttc gccggcgtcg ccagcggcat caacaacgcc gtcgcgcgca cggggtcgct
     6481 gctcgccgtt gcagcgctgc ccgcggcggt cgggctgagc ggcgcggact acgacgatcc
     6541 gacggcgttc aacgaggggt tccggatggc cttcgggatc tgtgccggcc tgctcgccgc
     6601 cggcggcgtc gtctcgtggt tgctgatccg caacccgttg ccggcaggcg atggacgggt
     6661 caccgcggcg gagcaccaag gcccggatca ctagcttcac tgatcacgcg actggcggcg
     6721 agccgcgtcc tcactcgctc gtgacgagac catgctcgac acagcgagcg acccagcccg
     6781 tcgggagcac ctggaccacc atccgacgac gacagaagcc gcagtaccgc ggtggctcga
     6841 gctcccgctc ccgtcggcac gccgagtggt cggtgtcgct cagcgctccg ccgcaccggt
     6901 cgcagtactc cggcacgtct ggagtcatag cgtcttggac agctccttga tcggcatctg
     6961 cagctcgtcc agcagatcca ggtccttggc cgggtcgcgg cccagggttg tcaggtagtt
     7021 gccgacgatc accgcgttga ccccgcccag caggccgtcc ctggtgccga gctccccgag
     7081 ggtgatctcg cggccaccgg cgtaacgcag cacggtgcgc ggcatggcga gccggaaggc
     7141 cgccacggtg cgcaaggcat cggcggatcc catcaccgga agcatcccga acggcgtacc
     7201 gggtcgcggg ttgaggaagt tgagcggcac ctcgtcgggg tccagctcgg cgagctgcga
     7261 cgcgagctcg gcgcgctgct ccaacgactc gcccatcccg accaggccac cgcagcacag
     7321 ctccatcccg gcctcgcgga ccagcaggca ggtttcccat cgttcgtcga acgagtgcgt
     7381 cgtcacgacg tgcgggaagt agctgcgagc ggcctcgagg ttgtggttgt agcggtgcac
     7441 tcccatcgct gcaagccggt cgacctgctc gggtgtgagc atgccgaggg agcacgcgat
     7501 gttgatgtcc acctccgtcc ggatcgcggt gatgccgtcc tcgacctgcc ggagcaggcg
     7561 ttcgtcaggt ccgcggaccg cggcaacgat gcagaactcg gtagcgccgg tggcggcggt
     7621 ctgcttggcc gccccgacca gcgaggggat gtccagccag gccgcgcgga ctggcgactc
     7681 gaacttgccg gactgagaac agaagtggca gtcctccgga catccaccgg tcttcaccga
     7741 gacgatgccc tcgacctcca cgtcgggtcc gcaccagcgc atccggacct gatgggcgag
     7801 ggcgagcagg gcaggaagcg cgtcgtcggg cagccggagc acctcgacga gctgcgcttc
     7861 ggacaacccg cgtccttcgt ccagagcctg caggcgggca acccccagga catcgacatc
     7921 gaccgtcacg gtggcaatct atcgagaccc ccttgatctc gacaggctcg atcagcggag
     7981 ttcagcggcc ttcgccgaac agcggtcctc ggcggtcgag cttgtcgaga ccccgaaact
     8041 gcaggcggag tgttgaccag gacccggcgt ctccgtcgtc cgggtccatc catcggtagc
     8101 ctgctgtgat gctgcagtcg gccgtggacg agcgactcgc cttcgaccgc gagcacctct
     8161 ggcatcccta cagctcgatg acctcaccgc tgccggcccg gtacgtcgtg agtgccgaag
     8221 gagtccggct gcggctctcc gacgggaccg agctgatcga tgccatgtcg tcgtggtggt
     8281 gcgcgatcca cggctaccgt cacccacacc tcgacgacgc cgtacgccga cagctggact
     8341 ccatgagcca cgtgatgttc ggcgggctca cccacgaacc ggccatcgag ctcgccaggc
     8401 gactgatcgg cctctcccct gagcccctcc agcacgtctt cttcgccgac tccgggtcgg
     8461 tctcggtcga ggtggcactg aagatggccg tgcagtacca gcgcggccgg agtcgcccac
     8521 ggcgcacccg gatgctcgcg ctgcgcggtg gctaccacgg cgacaccacg ggcgcgatga
     8581 gcgtcaccga cccggtcggt gggatgcaca gcatgttcgc cgacgtgctt ccacagcagg
     8641 tgttcgccga ccggccgccc gctccgggat cggacatcgc cgcctggtca tcgcatgtcc
     8701 gctcgctcgc cgctgcccac gccgacgaca tcgccgctgt cttctgcgaa ccggtcctcc
     8761 agggcgccgg cggcatgtac gtgtacgaac ctgcatgcct gaacgtgctc cgcgagatct
     8821 gtgacgagca cggcttcctg ctcgttctcg acgagatcgc caccggcttc ggtcgcaccg
     8881 ggacgttgtt cgcggccgag caggccggta tcgcccccga catcatgtgt gtcggcaagg
     8941 cgttgaccgg tggctacctc acgctggccg ccaccttgtg tacgaccgac gtcgccgccg
     9001 gcgtcgggtc gagtgaagct gcggcactga tgcacggccc gaccttcatg gccaacccgc
     9061 tcgcctgtgc ggccgcgtcg gccagcctgg acctgctgga cgaacgcgac tggcgagccg
     9121 acgtacaccg gatcgagtcc aggctgcggc agtacctcga gcctgcccgt cactcggcgc
     9181 aggtcgtcga tgtgcgtgtg ctcggagcaa caggcgtcat ccagcttgcc catcaggtcg
     9241 acgtcgtgcg agcatccgcg accgcagcga agcacggggt ctggctgcgg ccgttccgcg
     9301 acctcgtcta cacgatgccg ccgtacgtca ccagcgacgg cgagctcgac caggtctgca
     9361 ccgcgctgat cgccgcggca tcggcatgag tctcgacgac tggctggttc ggcggcgctc
     9421 gcgccgggag gaggccggcc tgcagcgctt cctgcacacg cggccggcaa cctccgacgt
     9481 catcgacctg gccggcaacg actacctcgg cctgagtcga gacccgcggg tgaccgcagc
     9541 ggccgccgaa gcagcgagcc gttggggtgc gggagctggg gcgtcacgtc tggtcaccgg
     9601 cacgcacgag atccacaacc ggctcgagca cgcgctcgcc gacttcaccg gacacgccgc
     9661 ggcattggtg ttctccaccg gttaccacgc caacctcggc gtcgtgtctg cgctcgccga
     9721 cgccgacacc ctgctcgtca gcgacgcgca cgttcacgca tcgctcgtcg acgcctgccg
     9781 gttggcgcgg gcaggcggcc tcacgatcac gccccacaac gacctgacgg cggtggagga
     9841 ggtcctgcgg acgcgttcgc acgatcgtgc gctggtcctc gtcgagtcgg tgtactccgt
     9901 cctgggcgac gccgccccgc tcgtcgagct ggcgggactc gtcgacgcgt atgacgcagt
     9961 gctgctcgtc gacgaggcgc atgccgtcgg tgtggccggc aaggacggga gcggcctcgt
    10021 cgcgacctgc ggactggccg gacggcctga cgtggtgacg acgatgacgc tgtcgaaggc
    10081 gctcggcagc caaggcggag ctgtgctcgg gtcgaccgcg ttggtcgacc acctggtcaa
    10141 caccgcccgg ccgttcatct tcgacaccgg actcgcgccg gcggctgccg ggggtgccct
    10201 ggccgcgctc ggcgtactcc gtgacgaacc cgggctgccg cacgacgtac tggccaccgc
    10261 ctgccggctc gccgatgcag ccggcgtcga ccaaccggcg gccgcagtcc tctcggtccc
    10321 gatgggatcc ccgcaagagg ccttgtcggc acagcactgg cttgccggcg agggtgtcca
    10381 ggtcggctgt ttccggccac catccgtccc ggacggcatg agccggctac gcgtgaccgc
    10441 gcgcgcggac ctcgccccgg ccgaggtcgc gctcgcgtgc cggctgctgc gtcacatcga
    10501 gcgccggcga tgaggttcgt cgtcgtcacc ggcaccggaa cgggtgtcgg caagacggtc
    10561 gccacggctg cgttcgtggc ggccgcgttg gaacagcgcc ggcgcgtgtg cgtcgtgaag
    10621 ccctaccaga ccggcgtcac ctccgacgag ccgagtgacg tcgcgtcggt cgtcgccctc
    10681 accggctgcc tggacgtgca cgagctcgtc cgcctcgacg acccgctggc tcccgagacg
    10741 gctgcgcggc tgcgcgccat cgagatccca accgtgcagg cgatcgcgga cgaggtcgtc
    10801 tcgtgcacgc gtcaggccga cgtgaccttc gtcgagggcg cgggtggcgt cgctgtccgg
    10861 ctcgacaccg aaggcggcac cctcatctcg ctcgccgcag cgttgcggtc gtacgggcac
    10921 gacgtcgacg tcgtcgtcgt gaccggcctc ggtctcggca cgttgaacca caccgcgctc
    10981 accgtgatgg cgctgcgggc ggagggtttc gaaccacatg gcctcgtctt cggtgacgtc
    11041 gtcgagccgc tcggacttgc ggagcggtgc aacgtcacgg acctgcctcg gatcactcag
    11101 gtgccggtgg tcgcgtcgat cccgcacggt gtcggccgcc ttcggcgcga ggagttcctc
    11161 gccgtggcct cgagctggat gctcgacggt cggctgttcg atcggtcgga gaccgatcag
    11221 cgatagaggc tggccgggag ctccgcaagc acatcggcca cgatgggcgc ggcgtcgtgt
    11281 gctccggacg tgccgttcgc gacgaagacc gcgaacgcca cgtcgccgcg atagccgatc
    11341 atccaggcgt tcgtaccggt gaccgacccg ttggcatact ccgccgtacc ggtcttcgcg
    11401 tgcaccggca ggccaggaag gttgaccgaa gtgccggtgc cgcgagtgac gacgagccgc
    11461 gtcatctgtt gaagcgaggc gatcgtgtcc gggttcagct cctccaggcg cttgccggga
    11521 agttcgccgg gcaacagcgt cggcgtacgg gagacaccgg agtccacggc ggccgccgtc
    11581 atcgccatca tcaacgggct ggccaagacc ttgccctggc cgatcatgtc ggccgctctc
    11641 gtcaccaggt cgctgtcagc cgggaccgaa ccgctgtagc tgtcgatgcc gagatcccac
    11701 ttctgaccga aaccgaaggc cgtcccttcc gccgcgagct gctgaccggt gatctcgtca
    11761 gcgaaggaca ccatggtcgt gttgcaggac gcggcgaacg cctgggcgaa ggtcgggttg
    11821 ggcccggtga tcccctgctc gtagttcctg aaccgcttgc caccgacaac ggtcgagtcc
    11881 gggcactcca cgcgcgtctg cggcgtcacg acgccggcgt cgagaagcgc ggcggagctg
    11941 accgtcttga aggtggagcc gggcgcgtac tgtcccacga acgccgtgtt gtacgacgtc
    12001 ggtcccggcg cgttggccgc cgccaggatc tcgccggtgc tcgccttcac caagacgacg
    12061 gcagtcgtgt ccgtcgcctc ctcgaccgcc ttctctgcgg cattctgcgc ccgcaggtcg
    12121 agcgtggtct ccagcggctc gcctgtcctc gcgcggcgga cgaacacctg gtcgagcgtg
    12181 ctgcctcccg gcgccttctc gaccaggtcg atggtgatgc caggcttccc cgccagccgc
    12241 tgctggaacg cgtactgcag cccgctcgtc cccacctcgt cggtcggcag cgcgagcggt
    12301 ccagcgttct tcaacgtgtc ctcggtggcc ggaccaacag tcccgagcac tgcccgcccc
    12361 caggcagagg tcggtgccag cgcccgcttc gcggtgtcga tggagatgcc ggggatctgg
    12421 agcaggtcgt cacgcagcgg ctcgtagtcc gcccgccgca ggtcgatgac ggagacgaac
    12481 cagccaggct tggacgcagc aacccggtct cggagcgccg aagggtcgat gtccagcagc
    12541 tgcgagaggt cgtcgtacgt cgccggacgc accttgcctg ccacgacgcc gacacggacg
    12601 atcgaccgct cgggcgtgag cgccaccccg ttgcggtcga ggatcggagc gcgcggcggc
    12661 agcgagcggt gccgcaccag cgtcgtgacg tcggtgaggt ccgggtggaa cgtcgacggt
    12721 gaccacgaga ccagccactg gccctggttg aggtccgccc tgatgagcgt ctcgtacgac
    12781 cactcgccgg ctcctgccag ctcgtgcgtg acgcgcgcgt gctccttgca ggagtcgctg
    12841 tcgcagtcga ggtcgccgga tggctcgacg gttgtcctaa tgattccgag ctcggcgacg
    12901 tgggcggcga tgtcgtgtgc agcaaccccg ggctcgtcca ccagaccgcg gaacgccgac
    12961 acggacgggt cagcccaggc ggtggcgaac tcgttgatga tcgcccctcc gctgtcagcc
    13021 gtgtcgggcg actccgtcgg gctgggctcg tccgagcccc ctgtgcacgc ggtcagcacg
    13081 aga
//
LOCUS       DATBFZ010000517         7862 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_6157514, whole genome shotgun sequence.
ACCESSION   DATBFZ010000517 DATBFZ010000000
VERSION     DATBFZ010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 7862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7862
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6157514"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1119
                     /locus_tag="VH419_15965"
     CDS             <1..1119
                     /locus_tag="VH419_15965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012919443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEY7045167.1"
                     /translation="LAIEPIDRYVVTQPGVYNAVLSRAVAEHGLFYPPDPSSWEFCSI
                     GGNVSTNSGGLCCVKYGVTTDYVLGLEVVLADGQVLRTGRRTVKGVAGYDLTRLFVGS
                     EGTLGVITEITLLLRPATERPLTVAAVYESAAAAAEAVSRVVRCGVVPSLLEFMDKVS
                     VQAVNEAFNMGISPEAGALVLAQSDAGGERATVEIARIAEVLEEAGAIEIAVADDPAE
                     GDLLLAARREVLTAFEKLGTTMIDDVCVPRTLLAELVERIAQVSADCGLTIGVVGHAG
                     DGNFHPTVVFDGQDASQVAKAQQAFDDIMQISLDLGGTITGEHGVGVLKMKLLERELG
                     DLSLQIHRDIKHALDPLSILNPGKVFDLQPRSAGEGQH"
     gene            1119..1649
                     /locus_tag="VH419_15970"
     CDS             1119..1649
                     /locus_tag="VH419_15970"
                     /inference="COORDINATES: protein motif:HMM:NF019614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HEY7045168.1"
                     /translation="MPSYIAFLRAVNVGGRMVKMATLRERLVDAGFTEVETFIASGNV
                     KVTTRLRSTTKVENALEAVMADWLDFEVPTLVRTPAQLSELYDAGARLSSPLSGRARH
                     YVAFLRDGPSASNVKRLESWEEPREAATVVGREVHLWLGSERPKLTNARMEKILDTVA
                     TARDWKTVAKLASAWV"
     gene            1804..3582
                     /locus_tag="VH419_15975"
     CDS             1804..3582
                     /locus_tag="VH419_15975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008361314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HEY7045169.1"
                     /translation="MLLATYQPDIESINGSLTASALVAALPLATLFVLLGVLRITAWI
                     SGLAALLVSILVATIGFDMPVDQALVVSSWGAAFGFFPIMWIVINAIWIYNMTVETGH
                     FDVLRRSFAAVSDDMRIQAIIIAFCFGALMEALAGFGTPVAISSFMLMGLGFRPLKAA
                     ALSLVANTAPVAFGALATPIVTLSTVTTAAVDPSQAGDFTTDRLGALVGHQTPVLALF
                     VPLALVFIVDGKRAIRQTWPAAVVCGVAFAIAQWLTSTYFSVQLTDIIASLVSALAVV
                     ALLRFWQPAEPYVEHADTVAEQDAGAGPAGGVSSGRRPVPTGVRTSTGGNAPGGTGTP
                     ATEAGISTEPVQDTPADVARAYAPYLVIIAIFSIVQIPAVKDELAKEPWTYLFQWPGL
                     DLVTAGGAPPADFTFNWLAAAGTLTIVAGLITIPIIGISAAGALKAYGATYRQLAPAI
                     VTVMAVLALAYVMNNSGQTGALGNWLATAGSAFALLSPVLGWLGVAVTGSDTSSNSLF
                     GALQVTAANQAGLDPALMAAANSSGGVLGKMISPQNLAIAAAAVGMTGREGDIFRRVV
                     GWSVLFLAVMCVLVYLQTTVLEWMLP"
     gene            complement(3664..4239)
                     /locus_tag="VH419_15980"
     CDS             complement(3664..4239)
                     /locus_tag="VH419_15980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012919453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEY7045170.1"
                     /translation="MTRALIVVDVQNDFCEGGSLAVSGGAEVAFRIGGLLHHWQESDP
                     PDRQYDVIVATRDHHIDPGPHFSDNPDFVDSWPPHCVAGTDGAAFHPNLDPQPFDAIF
                     LKGEHQAAYSGFEGKSSDGSSLADWLAAHEVHSVDICGIATDYCVRATALDAQQTGFA
                     TRVLTGYVAGVAPQTSEAALAEVEAAGVDLV"
     gene            complement(4283..5590)
                     /locus_tag="VH419_15985"
     CDS             complement(4283..5590)
                     /locus_tag="VH419_15985"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012919485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEY7045171.1"
                     /translation="MQPTGLLTDHYELTMVEAALRSGTAGRRSVFELFGRRLPSGRRY
                     GIVAGTGRLLDAIESFQFTTEQLDHLREHEVVDEPTLEWLATYRFDGDIWGYGEGDAY
                     FPFSPLLVVESTFAEAVVLETLLLSILNHDSAVASAASRMTVAAEDRPCIEMGSRRTH
                     EWAAGASARAAYIAGFASTSNLQAGLDHGVPTTGTAAHSFTLLHDSERDAFVAQVESL
                     GTGTTLLVDTYDVETAVKTAVEVAGNELGAVRLDSGDLGVLASRVRRQLDDLGATKTR
                     IIVTSDLDEFSIAGLAAAPVDGYGVGTSLVTGSGHPTCGFVYKLVARAAEPTPNAPLQ
                     SVAKKSADKISVGGRKWALRRRDRTGIAEAEVIGIGAAPVDDGDDRPLLLPLVQAGEI
                     VGREPLQTARERHDAAVAELPLDAHKLSRGEPAIPTEYLGRTV"
     gene            5688..5987
                     /gene="clpS"
                     /locus_tag="VH419_15990"
     CDS             5688..5987
                     /gene="clpS"
                     /locus_tag="VH419_15990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008358640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HEY7045172.1"
                     /translation="MTATPESVTVERPEVDETVLLDPPWITLVWDDPVNLMSYVTFVF
                     SDYFHYSRTKAHRLMLQVHTEGRAVVATGSREEMERDVQAMHSYGLWATLQKSDE"
     gene            5998..6555
                     /locus_tag="VH419_15995"
     CDS             5998..6555
                     /locus_tag="VH419_15995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012919487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2017 domain-containing protein"
                     /protein_id="HEY7045173.1"
                     /translation="MTEFRSRRKGGVYVELPPYAAGLLANLTRQLVELLSDGEATSSD
                     DPLEELLAIDQPRETPEDPALRRLLPDAHQQDPEAATEFRRLTERSLRQGKIADAAVV
                     LAAVGSPEDAVADLEFDLDRDQVRSWLRCLTDLRLTLAERLGVTANDDDYWESLDPSD
                     ERLPVHEIYQWLGYLLESLLDAART"
     gene            6604..7836
                     /locus_tag="VH419_16000"
     CDS             6604..7836
                     /locus_tag="VH419_16000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEY7045174.1"
                     /translation="MQFATSLYRQRADVLYHGYVRRDPMCLLQLAPGRRDPYAIYEQM
                     RAVAPIQPTRLGNWVTTSHVICRDVLRDRRLGVRPESVDPADEDFDLSFLDRNPPDHT
                     RLRRLAAPAFSPKHMAAYRPRIEKTVHALIDDMERQAAGGHSVDLVAAFAAPLPITVI
                     TDLLGIPDARATEFARYGATIGSALDGIKSMRHASALMAANRELEKLFGDLFALRRRE
                     PTDDVVSSLVAAEGDQISSDELLPLCVLLLIAGFETTVNLIGNAVLGLLDHPHQWSLL
                     REDRGLAPQAVEETLRFDPPVQRTARVALHDMELAGVTVRKDQFMVTLIGAANRDPEV
                     YDRPAEYDITRQPSVEHLAFSAGIHYCLGQPLARLEAEVAFAAIAERLPRLALAGPVR
                     RRNATTIRGPLTLPVNVS"
BASE COUNT         1203 a         2663 c         2692 g         1304 t
ORIGIN      
        1 ctggcgatcg agccgatcga ccggtacgtc gtcactcagc ccggcgtgta caacgccgta
       61 ctgtcccgcg cggtcgccga gcacggactg ttctacccgc cggacccgtc cagctgggag
      121 ttctgctcga tcggcggcaa cgtctcgacc aactccggtg gtctctgctg cgtcaagtac
      181 ggcgtcacga ccgactacgt gctgggactc gaagtcgtgc ttgcggacgg gcaagtcctg
      241 cggaccggcc ggcgtacggt caagggcgtc gccgggtacg acctgacccg gctcttcgtc
      301 ggcagcgagg gcacgctcgg cgtgatcacc gagatcacct tgctcctgcg cccggccacc
      361 gagcgcccgc tcaccgtggc ggcggtctac gagtctgcgg ccgctgcggc cgaggcggtc
      421 tcgcgtgtgg tgcgctgtgg cgtcgtaccg tccctgctgg agttcatgga caaggtcagc
      481 gtgcaagcgg tgaacgaggc cttcaacatg ggcatctcgc ctgaagccgg cgcactcgtg
      541 cttgcgcagt ccgatgccgg cggcgagcgc gccacggtcg agatagcgcg catcgccgag
      601 gtgctggagg aggcgggcgc catcgagatc gcggtggccg atgacccggc cgagggtgat
      661 ctgctgctcg ccgcccggcg cgaggtgctc accgcgttcg agaagctcgg cacgacgatg
      721 atcgacgacg tgtgcgtccc gcggactctt ctggccgagc tggtggagcg gatcgcgcag
      781 gtgtcggcgg actgcggcct caccatcggc gtggtcggtc atgcgggtga cggcaacttc
      841 caccccactg tcgtcttcga cgggcaagac gcgagccagg tcgccaaggc gcagcaggcg
      901 ttcgacgaca tcatgcagat cagcctcgac ctcggcggca ccatcaccgg tgagcacggc
      961 gtcggggtac tcaagatgaa gctgctcgag cgcgagctcg gagacctgtc gttgcagatc
     1021 catcgcgaca tcaagcacgc cctcgacccg ttgtccatcc tcaacccagg gaaggtgttc
     1081 gacctccagc ctcggtcagc gggcgaaggc caacactgat gccgtcgtac atcgccttcc
     1141 tgcgcgcggt caacgtgggt ggacggatgg tgaagatggc gacgctgcgt gagcggctcg
     1201 tcgatgccgg cttcaccgag gtcgagacgt tcatcgcaag cggcaacgtc aaggtgacca
     1261 cgaggcttcg cagcacgacc aaggtcgaga acgcgctcga ggcagtgatg gccgactggc
     1321 tcgacttcga ggttcccacg ctggtgcgca cacccgcgca gctgtcggag ctgtacgacg
     1381 ccggtgcccg gctgagcagt ccgctgtccg ggagagcccg gcactacgtc gccttcctgc
     1441 gtgacggccc gtctgcgtcg aacgtgaagc gcctcgagtc gtgggaagag cctcgtgaag
     1501 ccgcgaccgt ggtgggtcgt gaggtgcatc tgtggctcgg atccgagcgg ccgaagctca
     1561 ccaacgccag gatggagaag atcctcgaca cggttgccac tgcgcgcgac tggaagaccg
     1621 tggccaagct ggccagcgcc tgggtctgac ccgccgtcct gccgcgtctc ctggaggtac
     1681 ggggacgtgc atcggtggca ggtggcgcag tgagccgatc gaccacgcgt gagtggcgga
     1741 tccagtgcgg tgcggttagc gtgcgagagt tcgcaccgtt ccagacgcac cctggagctt
     1801 gtcatgctgt tggccacgta ccaacccgac atcgagtcga tcaacggctc gctcaccgcc
     1861 tcggccctcg tcgccgcact accgctcgcc accctgttcg tcctgctggg cgtgttgcgc
     1921 atcaccgcct ggatctccgg cctggccgcc ctgctcgtct ccattctcgt cgccacgatc
     1981 ggtttcgaca tgccggtcga ccaggcgctc gtcgtgtcga gctggggagc ggccttcggc
     2041 ttcttcccga tcatgtggat cgtcatcaac gcgatctgga tctacaacat gaccgtcgag
     2101 accggacact tcgacgtact tcgtcgctcg ttcgcggcag tcagcgacga catgcgcatc
     2161 caggcgatca tcatcgcgtt ctgcttcggt gcgctgatgg aggcgttggc gggtttcggc
     2221 accccggtcg cgatcagcag cttcatgctc atggggctcg gcttccggcc gttgaaggcg
     2281 gcggccctct cgctggtggc gaacaccgct ccggtcgcgt tcggagcgct ggccacaccg
     2341 atcgtgacgc tgtccacggt gacgacagcg gccgtcgacc ccagtcaggc cggggacttc
     2401 accactgatc ggctcggtgc tctcgtcggc caccagacgc cggtgctcgc gctgttcgtc
     2461 ccgctggccc tcgtcttcat cgtcgacggc aagcgggcca tccggcagac atggcccgcc
     2521 gcggtcgtgt gcggggtcgc gttcgcgatc gcgcagtggc tgacctcgac gtacttctcg
     2581 gtgcagctca ccgacatcat cgcgtccctg gtgagcgcgc ttgccgtcgt ggccctgctg
     2641 cgtttctggc agccggcgga gccgtacgtc gagcacgccg acaccgtggc cgagcaggat
     2701 gccggtgcgg gtccagcagg aggcgtgtcg tcgggccggc gtccggtccc cacgggcgtg
     2761 cggacctcca ccggcgggaa cgcgccgggt ggaaccggca cccccgccac cgaagccggc
     2821 atctcgaccg agcccgtgca ggacacgcct gcggacgtcg ccagggcata cgccccgtat
     2881 ctcgtgatca tcgcgatctt cagcatcgtg cagatcccgg cggtcaagga cgagctcgcc
     2941 aaggagccgt ggacgtacct cttccagtgg ccgggtctcg acctcgtcac cgccggcggc
     3001 gcgccgcccg cagacttcac gttcaactgg ctggctgcgg ctgggacgct cacgatcgtc
     3061 gctgggctca tcacgatccc gatcatcggg atctcggcgg cgggggcgct caaggcctac
     3121 ggcgccacct accgacagct cgcgccggcc atcgtcaccg tcatggcggt gctggcgctc
     3181 gcctacgtga tgaacaactc tgggcagacc ggagcgctcg gtaactggct ggccaccgcc
     3241 ggctccgcct tcgcgctgct ctcacccgtg ctcggctggc tgggcgtcgc ggtgacgggt
     3301 tcggacacgt cgtcgaactc gctgttcggt gcactccagg tcacggcggc caaccaggct
     3361 gggctggatc cggcactgat ggcggcggcg aactcctcgg gtggcgtgct cggcaagatg
     3421 atctcgccgc agaacctcgc gatcgcagcg gcagcggtcg ggatgaccgg acgcgaaggc
     3481 gacatcttcc gccgggttgt cggctggagc gtgctgttcc tcgccgtgat gtgcgtgctc
     3541 gtctacctgc agaccaccgt cctggagtgg atgctcccgt gatccgacgg tcgagcttgt
     3601 cgagaccacg cctttgatgc ctttgatctc gacgagctcg atcagcggac tgcgtgatcc
     3661 gggctagacg aggtcgacgc cggcggcttc gacctcggcg agggcggcct cggacgtctg
     3721 cggggccacg ccggcgacgt agccggtcag cacccgcgtg gcgaacccgg tctgctgtgc
     3781 gtcgagcgcg gtcgcccgca cgcagtagtc ggtcgcgata ccgcagatgt ccaccgagtg
     3841 gacctcatgt gccgcgagcc agtcggccaa cgatgagcca tcgctcgatt tcccctcgaa
     3901 gccggagtac gccgcctggt gctctccctt gaggaagatc gcgtcgaagg gctgcgggtc
     3961 gaggttcgga tggaaggcgg cgccgtcggt gcccgcgacg cagtgcggcg gccacgagtc
     4021 gacgaagtcg gggttgtcgg agaagtgcgg acctgggtcg atgtggtggt cgcgagtcgc
     4081 cacgatcacg tcgtactggc ggtccggcgg atcggactct tgccagtggt gcagcagacc
     4141 gccgatcctg aacgccacct cggcacctcc cgagacagca aggctgccgc cctcgcagaa
     4201 gtcgttctgc acgtcgacga cgatgagtgc tcgcgtcacg gttggttccc ttcggccgag
     4261 atccccacgt tagcggtccc gatcacacgg tccgcccgag gtactccgtc ggtatcgccg
     4321 gctctcccct cgacagcttg tgcgcgtcca acggcagctc cgcgacggcg gcgtcgtgtc
     4381 gctcgcgggc tgtctgcagc ggctcgcggc ccacgatctc cccggcttgc accaaaggca
     4441 gcaggagcgg ccggtcgtcg ccgtcgtcga ccggcgccgc accgatcccg atgacctccg
     4501 cctctgcgat gccggtcctg tcgcggcggc gcagggccca cttgcggccg ccgaccgaga
     4561 tcttgtcggc gctcttcttg gcgacagact gcaacggggc gttgggagtc ggttcagcag
     4621 ctcgtgccac caacttgtag acgaagccgc aggtcgggtg cccggaaccg gtgaccagcg
     4681 acgttccgac gccgtatccg tcgactggcg ccgcagcgag accggcgatg gagaactcgt
     4741 cgaggtcgct ggtgacgatg atccgcgtct tggtggcacc caggtcgtcg agctggcggc
     4801 ggacgcgact cgcgagcaca cccaggtcac cggagtcgag ccggacggcg ccgagctcgt
     4861 tccctgccac ctcgacggcc gtcttgactg ctgtctcgac gtcgtaggtg tcgaccagca
     4921 gcgtcgtacc agttccgagc gactcgacct gcgcgacgaa ggcatcgcgt tcgctgtcat
     4981 ggagcaaggt gaacgagtgc gcggcggtgc cggtcgtcgg tacgccgtgg tcgagcccgg
     5041 cctgcaggtt cgacgtggac gcgaagccgg cgatgtacgc cgcgcgcgca ctggcaccag
     5101 cggcccattc gtgcgtccgc cgggagccca tctcgatgca cggccggtcc tccgccgcga
     5161 cggtcatccg ggacgccgca gacgccacgg cactgtcatg gttgaggatc gacagcagca
     5221 gcgtctcgag taccacggct tccgcgaacg tcgactccac cacgagcagc gggctgaacg
     5281 gaaagtacgc gtcgccttcg ccgtatcccc agatgtcgcc gtcgaagcgg tacgtcgcga
     5341 gccattcgag tgtgggctcg tcgaccacct cgtgctcgcg cagatggtcg agctgctcag
     5401 tcgtgaactg gaacgactcg atcgcgtcga gcagccgacc cgtccccgcg acgatgccgt
     5461 agcggcgtcc cgacggaagc cgtcgcccga acagctcgaa gacgctgcga cggccggccg
     5521 tgccggagcg gagggctgcc tcgaccatcg tcagctcgta gtgatcggtc aacagaccgg
     5581 tcggctgcat gaacgtcagc gtagtgtgca gcgcctcacc cgaaagcggc tgccacgatg
     5641 ctcccgctgg tggtttccat gaggttggcc tctggaacaa tgggcgggtg actgcgacac
     5701 cggagtccgt caccgtcgaa cgaccggaag tcgacgagac ggtgcttctc gaccctccct
     5761 ggatcacgct cgtctgggac gacccggtca acctgatgtc gtacgtcacg ttcgtcttca
     5821 gcgactactt ccactactcg cgcacgaagg cgcacaggct gatgctgcag gtccacaccg
     5881 agggccgggc cgtggtcgca accggctcgc gggaggagat ggagcgagac gtccaggcca
     5941 tgcactccta cggattgtgg gcgaccctgc agaagagcga cgagtgaccg gttcgggatg
     6001 acggagtttc gcagtcggcg caagggcggg gtgtacgtcg agctgccgcc gtacgcggcc
     6061 ggccttctcg cgaacctgac gcggcagctc gtcgagctgc tcagcgacgg ggaggccacc
     6121 tccagcgacg acccgttgga agagctcctc gcgatcgacc agccgcgcga gactccggag
     6181 gatccggcgt tgcgtcggct gctgccggac gcccaccagc aggacccgga ggcggccacg
     6241 gagttccggc ggctgaccga gcgctccttg cggcagggca agatcgccga cgccgccgtc
     6301 gtgctcgccg ccgtcggctc gccggaggac gccgtcgccg atctggagtt cgacttggac
     6361 cgcgaccagg tgaggtcatg gctgcgttgc ctgacggatc tgcgtctcac ccttgccgag
     6421 cggctgggcg tgacggccaa cgacgacgac tactgggagt cgctcgaccc gagcgacgag
     6481 cggcttccgg tccatgagat ctaccagtgg ctcggctacc tcctcgagtc tctgctggac
     6541 gcggcgcgga cctagcggac cagggcgatg tcgaagagtc gtctctcggg gacgcgtcaa
     6601 gcgatgcagt tcgcgacgtc gctgtaccgg cagcgcgcgg acgtgctgta ccacggctac
     6661 gtgcggcgcg acccgatgtg tctgctccag cttgcgccgg gacgtcgtga cccgtacgcg
     6721 atctacgagc agatgcgcgc ggtggcaccg atccagccga cgagactcgg gaactgggtc
     6781 acgacgagcc acgtgatctg tcgcgacgtc ctcagggatc gccggctcgg cgtacgaccg
     6841 gagtccgtcg acccggcaga cgaggacttc gacctgtcgt tcctcgaccg caacccgccc
     6901 gaccacaccc ggctacgccg actcgccgcc ccggcgttca gcccgaagca catggcggcg
     6961 taccggccgc ggatcgagaa gaccgtgcac gcgctgatcg acgacatgga gcgacaggcg
     7021 gcaggtgggc actccgtgga cctcgtggca gcgttcgcgg caccgctgcc gatcacggtg
     7081 atcaccgacc tcctcgggat cccggacgcg cgggcgaccg agttcgcgcg gtacggcgcg
     7141 acgatcggca gcgcgctcga cggcatcaag tcgatgcggc acgcgtcggc tctgatggcg
     7201 gccaaccgcg agctggagaa gctgttcggt gacctcttcg ccctgcgccg tcgggagccg
     7261 acggacgacg tggtgagctc gttggtggcg gccgagggtg accagatcag cagcgacgag
     7321 ctgctgccgc tgtgcgtgct gctgctcatc gcaggcttcg agaccacggt caatctgatc
     7381 ggcaacgccg tactcggcct cctcgaccat ccgcaccaat ggtcgttgct gcgggaggac
     7441 cgcggtctgg caccgcaggc ggtggaggag acgttgcgct tcgacccgcc ggtgcagcga
     7501 acggcacggg tggcgctgca cgacatggag ctcgcgggtg tcacggttcg caaggaccag
     7561 ttcatggtga cgctcatcgg tgccgccaac cgcgatccgg aggtgtatga ccgtccggcg
     7621 gagtacgaca tcacccggca gccgtcggtg gagcatctcg cgttctcggc aggcatccac
     7681 tactgcctgg gtcagccgct ggccaggctc gaggccgagg tggcgttcgc ggcaatcgcc
     7741 gagcggctgc ctcgcctcgc actcgccgga ccggtccggc gtcgcaacgc gaccacgatc
     7801 cgcggcccgc tgacactgcc cgtcaacgtg agctgacgcg gcggctcgaa tcgtcgacgg
     7861 gc
//
LOCUS       DATBFZ010000518         4417 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_4516385, whole genome shotgun sequence.
ACCESSION   DATBFZ010000518 DATBFZ010000000
VERSION     DATBFZ010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 4417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4417
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4516385"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="VH419_16005"
     CDS             complement(<1..282)
                     /locus_tag="VH419_16005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEY7045175.1"
                     /translation="MTVITTPPPVEPAQDWTFPAYTETRLDNGLRVLRYDCPGQYVVS
                     ATLLFDLPLSAEPREQEGVAGLLGRCLTKGAGGRDAEQFADALALCGADL"
     gene            complement(279..1622)
                     /locus_tag="VH419_16010"
     CDS             complement(279..1622)
                     /locus_tag="VH419_16010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEY7045176.1"
                     /translation="MTDIPDVRYPIEARTLDNGLRVVVSPTPGLGVVAVNLWYDVGSR
                     HEQPHRQGFAHLFEHLMFQGSRHVGPGDHFSLLQGHGASLNATTSFDRTNYFESMPNG
                     VLDLALWLEADRLAYLDDVLTQENFANQVDVVAQERSQRMDNVPYGTVFEKALPLIFD
                     PEHPYGHLPIGNMEHLAAATLDEVAAFHAQFYMPNNAVLSIVGDVDVDAAFGKAVTYF
                     EHIEPGDAPKREIPPTLPPLDEARSLDHREDVPSPGVWFAFRLPLDAPDSRELAAVSL
                     ASSILGENETSRLYRRLVRQDRTALAAGLGLNTLIAGNSLGLGSVRALPGQDLDEIVA
                     AVDDVLHRFAVDGPSEHELTMARAQAERDWLDEMGTAAGRADAISGCTLLFDDPQSLN
                     ERLPLLRSITAEEVRAAAQTWLVPALNAQVRVHPEGPTAEAALAGEIDPSEGVSS"
     gene            1875..3266
                     /locus_tag="VH419_16015"
     CDS             1875..3266
                     /locus_tag="VH419_16015"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HEY7045177.1"
                     /translation="MPAPAAVASQPQPELLANAPSWSSTIAAVQAVPVVPVPTDRPPV
                     VTDAPASDRHKHGKPKARHHQKKRAVIKKTQHERIEQISANTSYDVPLAALTAYRNAA
                     NKVAATYPGCHLSWGVIAAIGQVESNHGRFGGAAVLSNGVTTPRIVGPELNGRGPVAA
                     IRDTDNGVYDGDKVWDRAVGPMQFIPSTWVNIGVDGDGDGHRNPSDFDDAALSTALYL
                     CNAGGDFSSLGQARSNVMRYNHSEEYVDLVLKIANAYDSGVVDVVPNDVAPPKQPAHK
                     PHHKHPGRHHHHGKPAPHRPQHPNQPGQQPDRPSQPKPHHPSQPKPNPQPKPDPKPDP
                     KPDPPKPDPPVVRSYKGTWDYTTGTKGTVRGTVVKSFTIPAELVGDANADGDTDAANL
                     ELEAIEGGTVTVTTTDGVVTGFVVNRQPPPPSTPSPSPTSPAPSSPATTPPATTGFSA
                     EPTPAAPAETTAP"
     gene            3762..>4417
                     /locus_tag="VH419_16020"
     CDS             3762..>4417
                     /locus_tag="VH419_16020"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY7045178.1"
                     /translation="MSGRDNHEVVVVGAGLAGLSCAIDLHTAGHDVVVLEASDAVGGR
                     VRTDVLDDELILDRGFQLLNPAYPALRARVDLGALDLQPFEAGLVVASHGHHTVIADP
                     LRAPRHTGRALSHVTGSVREKLRFARYAARVAAMSPQRIKSQPDVPWGEALDAAGVGG
                     KFRSSVIEPFLAGVLAENDKQTSRVFVDLLIRSFVRGAPALPARGMQALPEQLASRLP
                     "
BASE COUNT          709 a         1564 c         1490 g          654 t
ORIGIN      
        1 gaggtcggca ccgcacagcg cgagggcatc ggcgaactgc tcggcgtcgc gtcctcctgc
       61 gcccttcgtc aggcatcgcc cgagcagacc ggcgacaccc tcctgctccc gaggttcagc
      121 cgacagcggc aggtcgaaga gcaaggtcgc cgagacgacg tactggccgg gacagtcgta
      181 gcgcagcacc cgcagaccgt tgtcgagacg agtctcggtg tacgccggga aggtccagtc
      241 ctgcgccggc tcgaccggtg gcggtgtcgt gatcacggtc atgacgagac tccttcggac
      301 ggatcgatct cgccggccag ggcagcctcg gccgtgggtc cctccgggtg gacgcggacc
      361 tgggcgttca gcgccggcac cagccacgtc tgggcggcgg cacggacctc ctcggcggtg
      421 atgctgcgca gcaacggcag ccgttcgttg aggctctgcg ggtcgtcgaa caacagcgtg
      481 cagccggaga tggcgtctgc ccgcccggct gccgtgccca tctcgtcgag ccagtcacgc
      541 tcggcctggg cacgcgccat ggtcagctcg tgctcgctcg gaccgtccac ggcgaaccgg
      601 tggagcacgt cgtcgacggc ggcgacgatc tcgtccaggt cctggccggg caacgcccgc
      661 accgatccga ggccgagcga gttgccggcg atcagtgtgt tcaggccgag accggcggcg
      721 agcgcggtgc ggtcctgacg gaccaggcgg cggtagagcc ggctggtctc gttctccccg
      781 aggatgctgc tcgccaggct gacagcggcc agctctcggg agtcgggtgc atccagcggc
      841 aaccggaacg cgaaccacac gcctggcgac ggcacgtcct cgcggtggtc gaggctgcgc
      901 gcctcgtcca gcggcggcag ggtcggcggg atctcccgct tgggtgcatc accgggctcg
      961 atgtgctcga agtacgtgac cgccttgccg aaggccgcat cgacgtcgac gtcgccgacg
     1021 atcgagagca cggcgttgtt cggcatgtag aactgtgcat ggaacgccgc gacctcgtcc
     1081 agggtggcgg ccgccaggtg ctccatgttc ccgatcggca ggtggccgta cgggtgctcg
     1141 ggatcgaaga tcaacggcag cgccttctcg aagacggtgc cgtacggcac gttgtccatc
     1201 cgctggctgc gctcctgcgc cacgacgtcg acctggttcg cgaagttctc ctgcgtcagc
     1261 acgtcgtcga ggtaggccaa ccggtccgcc tcgagccaaa gggccaggtc gaggacaccg
     1321 ttcggcatcg actcgaagta gttggtccgg tcgaaggacg tcgtcgcgtt gagggaggcg
     1381 ccatggccct gcagcaagga gaagtggtcg cccggtccga cgtgccgcga cccctgaaac
     1441 atcaggtgct cgaagaggtg cgcgaagcct tggcggtggg gctgctcgtg acgggacccg
     1501 acgtcgtacc agaggttgac cgccaccacg ccgagaccgg gtgtcgggct gacgaccacg
     1561 cgaaggccgt tgtcgagggt gcgcgcctcg atggggtatc gcacgtcggg gatgtcggtc
     1621 acccgacgaa tctacctgcc gttcgaaccc gtgctcgtga ccgcggtcgt cccccggcgg
     1681 atgcgcgatc gggcagcgcg gagcgcatcg gcgtctcacc ttgcggacga aagacgatgc
     1741 ggtggtcatc cggtcggctg atgttcaccg gaacgtgtca cgcacaccga aagtgctgat
     1801 tacgcttgag ccacatggtg agagggcgat tctctatacg tcacggtggc ttgtgcattg
     1861 ccgtggctgt cctcgtgccc gcacccgctg ccgtggccag tcagccgcag ccggagctgc
     1921 tcgccaacgc cccttcgtgg agctccacga tcgccgcggt ccaggccgtt ccggtcgtgc
     1981 cggtgcccac cgaccggccc ccggtcgtca ccgacgcacc cgcatccgac cgccacaagc
     2041 acggcaagcc gaaggcgcgt caccaccaga agaaacgcgc agtcatcaag aagacgcagc
     2101 acgagcggat cgagcagatc agcgccaaca ccagctatga cgtgccgctg gccgcgctga
     2161 cggcataccg caacgccgcc aacaaggtcg ccgccactta ccccggctgc cacctcagct
     2221 ggggtgtcat cgccgcgatt ggtcaggtcg agtccaacca cggtcggttc ggcggcgcag
     2281 cggtcctctc caacggtgtg acgacgccgc ggatcgtcgg ccccgagctc aacggtcgcg
     2341 gcccggtcgc cgccatccgc gacaccgaca acggcgtgta cgacggtgac aaggtctggg
     2401 accgcgcggt cggcccgatg cagttcattc cgagcacgtg ggtcaacatc ggcgtcgacg
     2461 gcgacggtga cggccaccgc aacccgagcg acttcgacga cgccgcactc tccaccgcgc
     2521 tctacctgtg caacgccggt ggtgacttca gcagcctcgg gcaggcgcgg tcgaacgtga
     2581 tgcgctacaa ccactccgag gagtacgtcg acctcgtgct gaagatcgcc aacgcgtacg
     2641 acagcggcgt cgtcgacgtg gttccgaacg acgtggcgcc gcccaagcag cccgcgcaca
     2701 agccgcacca caagcacccg ggccggcacc accatcacgg caagcccgca ccgcatcggc
     2761 cgcagcaccc gaaccagccc ggccaacaac cggaccgccc gagccagccg aagccgcacc
     2821 acccgagcca gccgaagccc aacccgcagc ccaagccgga cccgaagccg gacccgaagc
     2881 ccgatccgcc caagccggat ccgccggtgg tcagaagcta caagggaacc tgggactaca
     2941 cgaccggcac aaagggcacg gtgcgaggaa cggtggtcaa gtccttcacg atcccggccg
     3001 agctggtggg tgacgccaac gccgacggcg acacggacgc cgccaacctc gagctcgagg
     3061 cgatcgaggg cggaaccgtc acggtcacca cgactgatgg agtggtcacc gggttcgtgg
     3121 tgaaccggca gccgcctccg ccgtcgacac cctctccgtc ccccacgagt cccgccccgt
     3181 cgagcccggc gaccacgccg ccggcgacga cgggattcag cgcagaaccc acgcccgccg
     3241 cccccgcgga gaccaccgcc ccctgacccc ttcgtcgagt gcgcaagacg tgggtgtgtt
     3301 gtgtgcgcag gttgtcggtg ttcagtgcgc agcccgccgc tgactcgacg ctgattacct
     3361 gcgcactcga tgaggtggag gggtggctgg gctcgcggcc gcgacgcccg gcagggcgtc
     3421 gcggcgcgag atccctgact gcgggtgggc agtcgggtgt ggtgcagaca tgcccttcct
     3481 cccggaccaa gggacgttga cggacgcgga cgtcacctgg tcgagcctct ccgtctctcg
     3541 ccggttcggc aggaaggaag gtcctccctc cagtgcgctc cactctacga agacctgcca
     3601 ccctgcgtag cggattcact acacatcgtc cgtttgggtg agtcgtttcg gctgaacggt
     3661 gctctttgcc ctgatctggt cctgcacacc tccctctcct ccctcatgat gggtcggcag
     3721 ccgatgtggc tgggatcggc gaggagaggg catgggacca cgtgagcggt cgtgacaacc
     3781 atgaggtcgt ggtcgtcggc gccgggctcg ccggcctcag ctgtgcgatc gacctgcaca
     3841 ccgccggaca cgacgtcgtc gtcctcgagg cgtccgacgc cgtcggcggc cgcgtccgta
     3901 ccgacgtcct cgacgacgag ctcatcctcg accgcggctt ccagctgctg aacccggcgt
     3961 accccgccct ccgagcccgg gtcgatctcg gggcgctgga cctgcaaccg ttcgaggccg
     4021 ggttggtcgt cgcgtcgcac ggccaccaca cggtgatcgc cgacccgctg cgcgcgcccc
     4081 ggcacaccgg cagggcgctc agccacgtga ccgggtcggt gcgcgagaag ctgcggttcg
     4141 cgcggtacgc cgcccgggtg gccgcgatgt cgccgcagcg gatcaagtcg cagcccgacg
     4201 tcccctgggg tgaggcgctc gatgccgccg gtgtcggcgg gaagttccgg agcagcgtga
     4261 tcgagccgtt cctcgccggc gtgctcgccg agaacgacaa gcagacgtcg cgggtcttcg
     4321 tcgacctgct gatccgttcc ttcgtccgcg gtgcgcccgc ccttcccgca cgcggcatgc
     4381 aggcccttcc cgagcagctg gcgtccaggt tgcccga
//
LOCUS       DATBFZ010000519         9496 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_4847679, whole genome shotgun sequence.
ACCESSION   DATBFZ010000519 DATBFZ010000000
VERSION     DATBFZ010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 9496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9496
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4847679"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1407)
                     /gene="glpK"
                     /locus_tag="VH419_16025"
     CDS             complement(<1..1407)
                     /gene="glpK"
                     /locus_tag="VH419_16025"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009072696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEY7045179.1"
                     /translation="MAEYIASIDQGTTSTRCMIFDHSGGVVSVAQMEHEQIFPRAGWV
                     EHDPMEIWNNTREVIGGALSKANLNSGNIKAVGITNQRETAVVWDKTSGKPVYNAIVW
                     QDTRTQKICEELAGDAGAEKYKAKVGLPLATYFSGPKVRWILDNVEGAREKAEAGDLL
                     FGNMDTWVLWNLTGGAENNGVHVTDVTNASRTMLMDLSSLSWDESIASDMQIPTSMLP
                     EIRSSSDVYGDCKPGVINGVPVAGILGDQQAATFGQACLETGMAKNTYGTGNFVLLNT
                     GTEQVASENGLLTTVCYKIGDNDAIYALEGSIAVTGSLVQWVRDNLGLISAAPEIEDL
                     AKGVEDNGGAYFVPAFSGLFAPYWRADARGALVGLTRYVNKGHIARAVLEATAFQSRE
                     VVDAMNADSGVALTELKVDGGMVQNETLMQFQADILGVDVVRPKVAETTALGAAYAAG
                     LATGYWQGEDDVRNNWGED"
     gene            complement(1425..2162)
                     /locus_tag="VH419_16030"
     CDS             complement(1425..2162)
                     /locus_tag="VH419_16030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HEY7045180.1"
                     /translation="MSFGEIVLSEIGGTGMLTLLGCGVVANVVLAKNKGFNGGFLMIN
                     FGWGIAVFCGVYVAYKTGAHLNPAVTFGILSSGSDTYGPDIDVDFANTIAYLIGEFAG
                     AFLGAVLAWAAYRDHFDDPESEPGAKLATFSTGPAIRNYANNLITEIIGTFVLVFVIL
                     SFGHTPTGLGPLAVALLVVGIGASLGGPTGYAINPARDLGPRIAHFLLPIRGKGDSDW
                     AYSWVPVAGPLIGGIIAGILANIVGYV"
     gene            2326..4038
                     /locus_tag="VH419_16035"
     CDS             2326..4038
                     /locus_tag="VH419_16035"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020576467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HEY7045181.1"
                     /translation="MPARLDGAQRAAAWDRLTSRKFDVLIIGGGVTGCGAALDAATRG
                     LSVALVEARDFASGTSSRSSKLFHGGLRYLEQFNFGLVREALRERELMLTRIAPHLVK
                     PVSFLYPLTHRGWERPYIGAGMTLYDTMGGARSVPRHRHFTRGGALRKVPALKRTALT
                     GALMYYDAQADDARHTLTVARTAATYGATILNSAEVIALERAGERIVGAHVRDVESGQ
                     EVEVEASVVVNATGVWTDDIQTLAGGRGRFHVRASKGVHIVVPRDRINSETGLILRTE
                     KSVLFVIPWGTHWIIGTTDTDWELDKAHPAASRADIDYILEHVNAVLAVPLSHEDIQG
                     VYAGLRPLLSGENESTSQLSREHAVARPQPGLISIAGGKYTTYRVMAADAIDAAEEDI
                     GAPLPPSVTSLIPLMGAEGYHAMVNQVERLARRADLPVWRMQRLLDRYGSLTEELLTL
                     AADDRSLLEPVDGAEEYLRIEILYAATHEGALHLDDVLARRTRISIETPDRGTTSAAA
                     AAEIIAPVLGWDHERMTNEVDAYAARVEAERRSQQADDDLAADAARIAFPDTREVAVG
                     RALD"
     gene            complement(4197..5234)
                     /gene="trpS"
                     /locus_tag="VH419_16040"
     CDS             complement(4197..5234)
                     /gene="trpS"
                     /locus_tag="VH419_16040"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020579492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEY7045182.1"
                     /translation="MTTTTTTTPATTMLPATRPRVLSGITPSGPLTLGNYLGALRRFV
                     DGQDTSEAYYFVADQHAMTTEHDPRRLTELTVQTAALYLASGLDPSRSTIFVQSHVPE
                     HVELAYLLECTAYVGELGRMIQFKEKGGRPRTRASLFTYPCLMAADILLYDTETVPVG
                     GDQDQHVELARDLALRFNREYGETFVVPELARAALAARVCDLVTPTAKMSKSAPDSAM
                     GVVRMLDAPDLIRRKVMRAVTDSDTDVRYDPEAKPGVSNLLDIMAVIQRTDPRALAGQ
                     FTSYGQLKRACADAVVDVLTPIQQRYAELLAAPAALHEALADGAAAARRQAATVVARA
                     RAAMGLVPVTR"
     gene            complement(5396..6457)
                     /locus_tag="VH419_16045"
     CDS             complement(5396..6457)
                     /locus_tag="VH419_16045"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEY7045183.1"
                     /translation="MSWWQRFDSFQRRHRWAGYPLGVVYKFGDDQGNYLAALLTYYAF
                     LSVFPLLLLGSAILGFVLQGNEHLQHQILDSTLARFPVIGDDLRAPGGLKGSGIAVAV
                     GILTSLYGGLGVANATQNALNVAWAVPRNRRPNPIMIRLRSLVLLAFSGFAIIATTLI
                     ANTTIGVSALGGDLQPGVRWLITIGTVLVNAAIFMVIFRLAAAHKHPIHQDIPGALFV
                     SVIWQLLQYVGSAYVSRVVQDQSATTGAFAVVLGMLAWIYLGAMAVIAAIEINVVLAN
                     RLYPRALLTPFTDNVDLTDADRLVYTRYAKAQGHKGFQSVDVTFDESFRQRRGQKMAA
                     EEPIEQRAEEVREDRDALP"
     gene            6585..7238
                     /locus_tag="VH419_16050"
     CDS             6585..7238
                     /locus_tag="VH419_16050"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009076012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEY7045184.1"
                     /translation="MLDTFVFDVDGTLVDTNYHHAIAWFRAFRRFDLTPPLWRIHRAI
                     GLGGDKLVGAVAGDDVEKRFGDDIRDGWSEEYAALMGEVRPFEGARALLEEVRKRHVG
                     VVFASSGPADHVEHYVDLLDARSIAQDWTTAEDAEESKPAPDLVQIAMAKVDAGSSIL
                     LGDSTWDAEAAKNAGIPVYAVRTGGFSVDELEEAGARRVYESLDEIRGDLDRILGDH"
     gene            complement(7241..7651)
                     /locus_tag="VH419_16055"
     CDS             complement(7241..7651)
                     /locus_tag="VH419_16055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020574201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HEY7045185.1"
                     /translation="MPKAPLPPEVLELLEQPNPAVITSVRSDGQPVSVATWYLMDGDR
                     ILVNMDAGRKRLAYLRDDPRVSVTALKLDDWYTHVSVQGRVAEMRDDDGLVDIDRLSK
                     HYTGHPYRRRNRPRVSAWIEIDRWHGWSDGAPTH"
     gene            7731..8834
                     /locus_tag="VH419_16060"
     CDS             7731..8834
                     /locus_tag="VH419_16060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY7045186.1"
                     /translation="MLEIDRTRLRELLRQEQAEYERLHPTSRQLYEDATHLFGKVPMT
                     WMNMWSGGFPLSFERARGNHVVDVDGLEYVDFALGDTGAMAGHSPRPTVRAIEVRIRD
                     MGGITTMLPTADASWVGNELARRFGLTLWSFTLSATDANRWAIRLARAVTGRPKIICF
                     SYSYHGSVDETHTFSAGPGGATAAWGLRPDILVIGKSIGGGIPCGAYGITEDVGLAVD
                     ALVARGEADVEDVGGVGGTLAGNAMSVAAMRATLSEVLTEDAFADMIDRATGFTEGVR
                     SVIERCRLPWSITQLGARTEYRFTPDPPRDGESSAAVADTELDTYLHLGLANRGILMT
                     PFHNMALMCPTTSDDDVDRHTTAFGDLVDTLVG"
     gene            complement(9142..>9496)
                     /locus_tag="VH419_16065"
     CDS             complement(9142..>9496)
                     /locus_tag="VH419_16065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020574128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7045187.1"
                     /translation="NTDAVDYTVAGADWSPYNTDLHTSCGDYTAVAVREVLDTGSGDT
                     YFQDLPGQSFNVKNLPNGVYYISVEANPFGVLQETNTRNNNSLRKIILKGTPEHRRLI
                     VPRKGIIDESSWYSY"
BASE COUNT         1600 a         3124 c         3185 g         1587 t
ORIGIN      
        1 gtcctcgccc cagttgttgc ggacgtcgtc ctcgccttgc cagtagcccg tggccaggcc
       61 tgctgcgtac gccgctccaa gtgctgtcgt ctcggccacc ttcggtcgga cgacgtcgac
      121 gccgaggatg tcggcctgga actgcatgag cgtctcgttc tggaccatgc cgccgtcgac
      181 cttgagctcg gtcagcgcca cgcccgagtc ggcgttcatc gcgtctacga cctctcggct
      241 ctggaaggcg gtggcttcca ggaccgcgcg agcgatgtgg cccttgttga catagcgggt
      301 caacccgacg agcgcaccac gggcgtcggc gcgccagtac ggcgcgaaca ggccggagaa
      361 cgccggcacg aagtacgcac caccgttgtc ctcgacgccc ttggcgagat cctcgatctc
      421 gggggcggcg ctgatgagtc cgaggttgtc gcggacccac tgcacgagcg atccggtgac
      481 ggcgatcgag ccttcgaggg cgtagatcgc atcgttgtca ccgatcttgt agcagaccgt
      541 cgtcaacaag ccgttctccg acgcgacctg ctccgtacct gtgttgagga ggacgaagtt
      601 gccggtgccg taggtgttct tcgccatgcc cgtctcgagg catgcctgtc cgaaggtcgc
      661 cgcctgctgg tcaccgagga tgccggcgac cgggacaccg ttgatgacgc cgggcttgca
      721 gtcgccgtac acatcggacg acgacctgat ctcgggcagc atcgacgtgg ggatctgcat
      781 gtcggacgcg atgctctcgt cccacgacaa cgacgacagg tccatcagca tcgtgcggct
      841 ggcgttcgtc acgtcggtga cgtgcacacc gttgttctcc gcaccgccgg tcaggttcca
      901 aagcacccag gtgtccatgt tgccgaagag caagtcaccg gcctcggcct tctcccgtgc
      961 tccctcgaca ttgtcgagga tccagcggac cttcggtccg gagaagtacg tcgccagggg
     1021 gagacctacc ttggccttgt acttctccgc gcccgcgtcg cccgccagct cttcacagat
     1081 cttctgggtg cgggtgtcct gccacacgat ggcgttgtag acgggcttgc cgctggtctt
     1141 gtcccagacc accgccgtct cgcgttggtt ggtgatgccg acggccttga tgttgcccga
     1201 gttgagattc gccttcgaca aggcgccgcc gatcacctca cgggtgttgt tccagatctc
     1261 catggggtcg tgctcgaccc atcccgcgcg cgggaagatc tgctcgtgct ccatctgcgc
     1321 gacggagacg acgcccccgg agtggtcgaa gatcatgcat cgcgtactgg tggtgccctg
     1381 gtcaattgat gcgatgtatt cagccataga aaatcacggt ccgatcagac atagccgacg
     1441 atgttcgcga ggattccggc gatgatgccg ccgatgagcg ggcccgcgac ggggacccag
     1501 gagtaggccc agtcgctgtc acccttgccc ctgatgggca ggaggaagtg tgcgatacgc
     1561 ggtccgaggt cacgggccgg gttgatggcg tagccggtcg gcccaccgag tgaggcaccg
     1621 ataccgacca ccagcagtgc gaccgcgagc gggcccagtc cagtcggtgt gtgcccgaag
     1681 gacagaatga cgaagaccag cacgaacgtg ccgatgatct cggtgatcag gttgttggcg
     1741 tagttacgga tcgcgggacc ggtcgagaac gtggcgagct tcgcgcccgg ttcactttct
     1801 gggtcgtcga agtggtcccg gtacgccgcc caggccagca cggctcccag gaaggcaccg
     1861 gcgaactcgc cgatgaggta ggcgatggtg ttggcgaagt cgacatcgat gtccgggccg
     1921 tacgtgtctg aaccggatga caggatgccg aacgtgaccg ccgggttgag gtgcgcgccg
     1981 gtcttgtagg cgacgtacac gccgcagaag acggcgatgc cccacccgaa gttgatcatc
     2041 aggaagccgc cgttgaaacc tttgttcttg gcgaggacga cgttggcaac gacgccacat
     2101 ccgagcaacg tcagcatgcc ggtgccgcca atttcgctga gcacgatttc gccgaaactc
     2161 atgtgtccct ccgtacaaga aaggacgagc aggttaccct ccccgacctc tgcgcgaatg
     2221 gttagtgtgt aagacactag gggcggcgtt cactgacggt caacgacgcc tttccttggc
     2281 aaagtccgcg tctcgccata cgcaacggag gggtgttcca tgccgatgcc agcacgactc
     2341 gacggagccc agcgggccgc ggcgtgggac cggttgacgt cgaggaagtt cgacgtcttg
     2401 atcatcggag gtggtgtcac cggttgcggc gctgcactcg acgcggcaac gaggggattg
     2461 tcggtggcgc tcgtcgaggc gcgtgacttc gcgtcgggaa cctcatctcg ttcatcgaag
     2521 ctgttccacg gcggccttcg ttacctcgag cagttcaact tcggactggt ccgagaagcg
     2581 ctgcgcgaac gcgagctgat gctcaccagg atcgcacccc atctggtcaa accggtgtct
     2641 ttcctctacc cgctgacaca tcgtggctgg gagcgtccgt acatcggcgc cggcatgacg
     2701 ctctacgaca cgatgggcgg cgcacgatcg gttccccgcc accgtcactt cacccgaggc
     2761 ggggcgctcc ggaaggtccc cgcgctgaag cggacagcgc tgaccggcgc cctcatgtac
     2821 tacgacgcac aggccgacga cgcccgccac acactcaccg tcgcgcgcac cgcggcgacc
     2881 tacggtgcga ccatcctcaa ctcggccgag gtcatcgccc tcgagcgggc cggtgaacgc
     2941 atcgtcggcg cccacgtgcg tgacgtcgaa tccgggcaag aggtcgaggt cgaggcgtcg
     3001 gtcgtcgtca acgccaccgg tgtctggacc gacgacatcc agacgctcgc tggcggcagg
     3061 ggcagatttc acgtgcgggc atccaaaggt gtgcacatcg tcgtgccgcg cgaccggatc
     3121 aactccgaga ccgggctcat cctgcgcacg gagaagtccg tgttgttcgt gatcccgtgg
     3181 ggcacccact ggatcatcgg cacgaccgac accgactggg agctcgacaa ggcacaccct
     3241 gccgccagca gggctgacat cgactacatc ctcgagcacg tcaacgccgt cctcgccgtg
     3301 ccgctgtcac acgaggacat ccaaggggtg tacgccggcc ttcgaccgct gctgtcagga
     3361 gagaacgagt cgacgtccca gctgtcacgt gagcacgccg tcgcccggcc acagcctgga
     3421 ctcatctcca tcgccggcgg caagtacacg acgtaccggg tgatggcggc tgacgccatc
     3481 gacgccgccg aggaggacat cggtgcaccg ctgccgccga gcgtcaccag cctgatcccg
     3541 ctgatgggcg ccgagggcta ccacgcgatg gtcaaccagg tcgagcggct ggcccgacgg
     3601 gcagacctgc cggtctggcg aatgcagcgc ctgctcgacc gctacggttc gctcaccgag
     3661 gagctgctca ccctcgccgc ggacgaccgg agcctgctcg agccggtcga cggcgccgag
     3721 gagtacctgc ggatcgagat cctctacgcc gccacccacg aaggcgcgct gcatctcgac
     3781 gacgtcctcg cccggcgtac ccggatctcg atcgagacac ccgaccgtgg gaccacgtcc
     3841 gcggccgctg cggccgagat catcgcaccg gtcctgggct gggaccacga gcggatgacg
     3901 aacgaggtcg acgcgtacgc cgcgagggtg gaggcagaac gccgatccca acaggccgac
     3961 gacgacctgg ccgccgacgc cgcccgcatc gcgttccccg acacccgcga ggtggcggtc
     4021 ggcagagcac tcgactagct tcgccggttt ggttctgtcg accgaacacc gcggatcccg
     4081 aggtcctcga tctcgacacg ctcgatcagc gggctgcggc aatccggcgg gctgcggcct
     4141 tccggcggtc gagcctgtcg agaccccgcc ggcgccctcg atctcgacac gctcgatcag
     4201 cgggttaccg gtaccaggcc catggcggcg cgagcccgag ccacgaccgt cgctgcctgg
     4261 cgccgagcgg cggcagcacc gtcagccaac gcctcgtgga gcgccgctgg ggccgccagc
     4321 agctcggcgt agcgctgctg gatcggcgtc agcacgtcga cgaccgcgtc cgcgcacgca
     4381 cgcttgagct ggccgtacga cgtgaactgt ccggccagcg cgcgcgggtc ggtccgctgg
     4441 atcacggcca tgatgtccaa caggttggac actccgggct tggcctctgg gtcgtaccgc
     4501 acgtcggtgt cggagtcggt gacggcgcgc atcaccttcc gccggatcag gtccggcgcg
     4561 tccaacatcc gcaccacgcc catcgccgag tccggcgccg acttgctcat cttcgcggtc
     4621 ggggtgacga ggtcacagac ccgtgccgcc agtgcggccc gcgccagctc cggcaccacg
     4681 aaggtctcgc cgtactcgcg gttgaaccgc agggcgaggt cacgggcgag ctctacgtgc
     4741 tggtcctggt cgcctccgac cggcaccgtc tcggtgtcgt agagcaggat gtcagcggcc
     4801 atcaggcagg gataggtgaa gaggctggcc ctcgtcctgg gacggccgcc cttctccttg
     4861 aactggatca tccgtccgag ctcaccgacg tacgccgtgc actcgagcag gtaggcgagc
     4921 tcgacgtgct cgggcacgtg ggactggacg aagatcgtcg atcggctcgg gtcgagaccc
     4981 gatgccaggt agagcgcggc ggtctgcacg gtgagctcgg tcagccgtct cgggtcgtgc
     5041 tccgtggtca tggcgtgctg gtcggcgacg aagtagtacg cctcgctcgt gtcctgcccg
     5101 tcgacgaacc ggcgcagcgc cccgagatag ttcccgagcg tgagcggccc gctgggcgtg
     5161 atgcccgaca acaccctcgg cctggtggcc ggcagcatcg tcgtcgctgg tgtggtggtc
     5221 gtggtcgtgg tcatggcgtg tcctcggtga cattgtcggt gagttgtccc agggtggccg
     5281 ctgacaacac gacagccgcc cacctggggc ggctgggagt gaacagtctc gatctggcag
     5341 cggcccctag gccgaccacc acccctgcgt cgtcgcgaac gtcgagaaca tgcactcatg
     5401 gtagagcatc gcggtcctca cgtacctcct cggcccgttg ctcgatcggt tcctctgccg
     5461 ccatcttctg accacgccgc tgacggaacg actcgtcgaa cgtcacgtcg acgctctgga
     5521 agcccttgtg gccttgggcc ttcgcgtatc tggtgtagac gagccggtcg gcgtcggtga
     5581 ggtcgacgtt gtcggtgaac ggcgtgagca gtgcgcgtgg gtacagcctg ttggcgagca
     5641 ccacgttgat ctcgatggcc gcgatcaccg ccatcgcgcc gaggtagatc cacgccaaca
     5701 tgccgagcac caccgcgaac gcgccggtcg tggcgctctg gtcctgtacg acgcggctga
     5761 cgtacgccga gccgacgtac tgcagcagct gccagatgac cgagacgaac aacgcaccag
     5821 ggatgtcttg atggatcgga tgcttgtgcg ccgcggccag ccggaagatg accatgaaaa
     5881 tcgcggcgtt gacgagcacg gtcccgatgg tgatgagcca tcgcacaccg ggctgcaggt
     5941 caccgccgag cgccgaaacc ccgatcgtgg tgttcgcgat cagcgtggtc gcgatgatgg
     6001 caaagccgga gaaggcgagc agcacgaggc tccgcagccg gatcatgatc gggttgggcc
     6061 ggcggttgcg cggcaccgcc caggcgacgt tgagcgcgtt ctgggtggcg ttcgcgacac
     6121 cgagaccgcc gtacagcgag gtgaggatac cgacggccac ggcgatcccg ctgcccttca
     6181 gaccgccggg tgcccgcagg tcgtccccga tcaccgggaa acgcgccaac gtggagtcga
     6241 ggatctggtg ttggaggtgc tcgttcccct gcaggacgaa gccgaggatg gccgagccca
     6301 gcagcagcag cgggaagacc gagaggaatg cgtagtacgt gagcagcgct gccaggtagt
     6361 tgccctggtc gtcaccgaac ttgtagacga cgcccagcgg atagccggcc cagcggtggc
     6421 gccgctggaa gctgtcgaac cgctgccacc acgacacgtg tgccgccttt ccgcgcgtcg
     6481 tccacccgcg ctcccgtcaa gtcaagcatg gcgaaaacaa agacggggta cagcgcggtt
     6541 ggcgagctcg aagagccgcc taccgccgag ctccgggaga tgcagtgctg gacacattcg
     6601 tcttcgacgt cgacgggacg ctcgtcgaca ccaactatca ccatgcgatc gcatggttcc
     6661 gggcgttccg ccgcttcgat ctgacaccac cgctgtggcg catccaccgg gccatcggcc
     6721 tgggtggcga caagctcgtc ggcgcggtcg cgggtgacga cgtcgagaag cggtttggcg
     6781 acgacattcg ggacggatgg tccgaggagt acgccgcctt gatgggcgag gtgcgaccgt
     6841 tcgagggagc acgtgcgctg ctcgaggagg tccgcaagcg tcacgtcggc gtcgtgttcg
     6901 ccagctcagg ccccgccgac cacgtcgagc actacgtgga cctgctcgat gcacgctcga
     6961 tcgcccagga ctggacgacg gccgaggacg cggaggagag caagcccgcg ccggacctgg
     7021 tgcagatcgc catggccaag gtcgacgccg ggtcgtcgat cctgctcggg gactcgacct
     7081 gggacgctga ggcggcgaag aacgccggca ttcccgtgta tgccgtccgg accggcggct
     7141 tctccgtcga cgagctcgaa gaggccggcg cgcggcgggt ctacgagtct ctcgacgaga
     7201 tccggggcga cctcgaccgc atcctcggcg accactgaga tcagtgggta ggggcgccgt
     7261 cggaccaacc gtgccagcgg tctatctcga tccatgcact gacacggggt ctgttgcgcc
     7321 gacggtacgg gtggccggtg tagtgcttcg acagccggtc gatgtcgacg agcccgtcgt
     7381 cgtcgcgcat ctccgcgacc ctgccttgga cgctgacgtg cgtgtaccag tcgtcgagct
     7441 tcagggcggt caccgagacg cgagggtcgt ctcgcaggta ggccagcctc ttgcgaccgg
     7501 cgtccatgtt gacgaggatg cgatcaccgt ccatgaggta ccaggtggca acggagaccg
     7561 gctggccatc ggaccggacc gacgtgatca ccgccgggtt gggttgttcc agcagctcga
     7621 ggacctcagg cgggagtggc gctttcggca tgtcagctcc tgattcgtgg gcgacgacgt
     7681 gttcactgtg ccactgttcc ctgaacgagc ggtgagcaag cgaggtcccg atgctggaga
     7741 tcgaccgcac ccggttgcgt gagctgctgc ggcaggagca agcggagtac gaacgactgc
     7801 atcccacgag cagacagctc tacgaggacg ccacccacct gttcggcaag gttccgatga
     7861 cgtggatgaa catgtggtcc ggcgggttcc cactgtcgtt cgagcgtgcc cgcggcaatc
     7921 acgtcgtcga cgtggacggt ctggagtacg tcgacttcgc gctcggcgac accggcgcga
     7981 tggccggtca ctcacccagg cccaccgtgc gggcgatcga ggtgcgcatc cgcgacatgg
     8041 gcgggatcac gacgatgcta ccgacggcag atgccagctg ggtcggcaac gagctggccc
     8101 ggcgcttcgg tctcacactc tggtcgttca cgttgtcggc caccgacgcg aaccgatggg
     8161 cgatccggct ggcccgtgcg gtgacgggcc gcccgaagat catctgcttc agctacagct
     8221 accacggctc ggtcgacgag acgcacacgt tctcggcggg tcctggcggt gcgaccgcgg
     8281 cctgggggtt gcggccggac atcctcgtca tcgggaagag catcggcgga ggcatcccgt
     8341 gtggcgccta cggcatcacc gaggacgtcg gcctcgccgt cgatgccctg gtggcacgtg
     8401 gtgaggccga tgtcgaggac gtcggcggag tcggtgggac gttggccggc aacgccatgt
     8461 cggtcgcggc gatgcgtgcg accttgtcgg aggtgctgac cgaggacgcg ttcgccgaca
     8521 tgatcgaccg cgccaccggc ttcaccgaag gcgtccgatc ggtgatcgaa cgctgccggc
     8581 tgccgtggtc gatcacccag ctcggtgccc ggacggagta ccggttcacc ccagatccgc
     8641 cgagggacgg ggagtcgtca gccgcggtcg ccgacaccga gctcgacacg tacctgcacc
     8701 ttggcctggc caaccgcgga atcctgatga cgccgttcca caacatggcc ctgatgtgtc
     8761 cgacaacttc cgacgacgac gtcgaccgcc acaccaccgc attcggcgac ctcgtcgaca
     8821 ccctcgtcgg ctgacctacc cgccgaccaa ccgagtcgtc ggcggtggtt tgccttggta
     8881 gccatgcaaa cggacgggta gccaggcaga tctgccttgg gagggatggc actgcctggt
     8941 tagccatgca aaccgcaccc gcggacgagc ggatgtccga aacccgcgag tcacaacatc
     9001 aagcacaagg aacggagcgc cggctgacga gctcagcggc accgaacgtc tcccgctctt
     9061 cactgccgat ccgcctcagc cgaggacgac cgagggtccg gcgaacagag cgacccagcg
     9121 ccaccggcgc agacaccggg ctcagtagga gtaccaggac gactcgtcga tgatgccctt
     9181 gcgagggacg atgagacgac ggtgctccgg ggtgcccttc aagatgatct tgcggaggct
     9241 gttgttgttc cgggtgttgg tctcctggag aacgccgaac gggttggcct ccaccgagat
     9301 gtagtacacg ccgttgggca ggttcttcac gttgaaggac tggccaggca ggtcctggaa
     9361 gtaggtgtcg cccgacccgg tgtcgagcac ctcccgcacc gccaccgcgg tgtagtcgcc
     9421 gcaggacgtg tgcagatcgg tgttgtacgg actccagtcg gcgcccgcga cggtgtagtc
     9481 gaccgcgtcg gtgttg
//
LOCUS       DATBFZ010000520         5024 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_4518378, whole genome shotgun sequence.
ACCESSION   DATBFZ010000520 DATBFZ010000000
VERSION     DATBFZ010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 5024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5024
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4518378"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="VH419_16070"
     CDS             complement(<1..297)
                     /locus_tag="VH419_16070"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEY7045188.1"
                     /translation="MTDFTHTWDGVEIAADEPRGSTVVVRRHSAAGELELLLLHRNAR
                     GPDFAGDWAWTSPAGARQPGEVVHAAAMRELAEETGLTDVPIFAVDLSGSWALFG"
     gene            complement(303..848)
                     /locus_tag="VH419_16075"
     CDS             complement(303..848)
                     /locus_tag="VH419_16075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HEY7045189.1"
                     /translation="MARRHQHEPDDADRDLGPVADPESVARTIVLAKLTASAKSRQEL
                     ADALASRNVPDGVANRVLDRFEQVGLVDDTAFAEAWVASRQRSRGLSRRALRHELHRK
                     GVNDEAITAALEQVDSESERAAATELVERKLASTRRLDPDRRTRRLLGLLARKGYGPA
                     LATSVVREALASEDVSDRTLG"
     gene            complement(885..1511)
                     /locus_tag="VH419_16080"
     CDS             complement(885..1511)
                     /locus_tag="VH419_16080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7045190.1"
                     /translation="MDHKLFENQRGQFDVQPGFVQPEVRPVPARILSIHIKPPTRSMH
                     KFDIEVEGNHNYFVDGVMVHNSPETTTGGRALKFYSSVRLDVRRIESLKDGTDFVGNR
                     TRVKVVKNKVASPFKQAEFDIMYGQGISREGGLIDVGVETGLVRKAGAWYTYEGDQLG
                     QGKENARTFLRDNPDLANELEKKILEKLGIGASLDDEGAPSEPTDIDF"
     gene            complement(1557..2108)
                     /locus_tag="VH419_16085"
                     /pseudo
     CDS             complement(1557..2108)
                     /locus_tag="VH419_16085"
                     /inference="COORDINATES: protein motif:HMM:NF015141.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LAGLIDADG endonuclease"
     gene            complement(2169..3017)
                     /gene="recA"
                     /locus_tag="VH419_16090"
                     /pseudo
     CDS             complement(2169..3017)
                     /gene="recA"
                     /locus_tag="VH419_16090"
                     /inference="COORDINATES: protein motif:HMM:TIGR02012.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
     gene            complement(3320..3514)
                     /locus_tag="VH419_16095"
     CDS             complement(3320..3514)
                     /locus_tag="VH419_16095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016667497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3046 domain-containing protein"
                     /protein_id="HEY7045191.1"
                     /translation="MRVTEFWSRMEAALGSTYARVWAREHVIEGLGGRTVVEAFDEGY
                     DAKTVWREVWKVLELPPRER"
     gene            complement(3568..4401)
                     /locus_tag="VH419_16100"
     CDS             complement(3568..4401)
                     /locus_tag="VH419_16100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002517211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HEY7045192.1"
                     /translation="MTSQPEVQPRLASSHVDGAGAVVVQDATTARRRLSWEIVIVLGL
                     SLGASATYAVISLVGKLTEPGRLSEQTATLNQSVTPRPLLDLTYQLAGIFFALMPVAL
                     VIFLLSAGGASAIRQLGVDVRSRAGLGRDLAWGAGLAALIGIPGLGLYYLAKALDLNA
                     TIVPSALNDHWWTLPVLVLSAAQNAVLEEVVVVGYLITRLRQLGWTPVWATAASAVLR
                     GSYHLYQGFGAFLGNAVMGLLFGTFFIRRGRVLPLIVAHTILDVVAFVGYAVLKDRLN
                     L"
BASE COUNT          807 a         1717 c         1648 g          852 t
ORIGIN      
        1 gccgaacagc gcccacgacc ccgagaggtc gaccgcgaag atcggcacgt cggtcagccc
       61 ggtctcctcc gcgagctccc gcatcgccgc cgcgtggacc acctcgcccg gctgcctggc
      121 gccagcgggt gaggtccagg cccagtcgcc cgcgaagtcc gggccccgtg cgttgcgatg
      181 cagcaggagc agctccagct cgccggcggc gctgtgacgg cgtacgacga cggtggaacc
      241 tcgaggctcg tccgcggcga tctccacacc gtcccacgtg tgcgtgaagt ccgtcatggc
      301 gctcacccta gggtgcgatc gctgacgtcc tctgaggcca gggcctcgcg cacgaccgag
      361 gtggccagcg ccggtccgta gcccttgcgg gcgagcagcc cgagcagtcg acgggtacgc
      421 cgatcggggt cgagccgacg ggtgctcgcc agcttgcgct ccacgagctc ggtggcggca
      481 gcgcgctcgg actcggagtc cacctgttcc agcgccgctg tgatcgcttc gtcgttcacg
      541 cccttgcgat gcagctcgtg tcggagtgcg cggcgcgaca gcccgcgcga ccgctgtcgc
      601 gacgccaccc acgcctcggc gaaggctgtg tcgtcgacca accccacctg ctcgaaccgg
      661 tcgaggaccc ggttggccac gccgtcgggg acgttccggg acgccagcgc gtcggcgagc
      721 tcttgccgcg acttggccga cgcggtgagc ttcgccaaca cgatcgtcct ggccaccgac
      781 tcgggatcgg cgaccggtcc gaggtcgcgg tcagcgtcgt cgggttcgtg ctgatgtcgt
      841 cgtgccacag ctgtccaact cgaggacggc ggatacgtcg tgggctagaa gtcgatgtcg
      901 gtcggctcgg atggggcacc ctcgtcgtcg aggctcgcac cgatgccgag cttctcgagg
      961 atcttcttct ccagctcgtt ggcgaggtcg gggttgtcgc gcaggaacgt ccgggcgttc
     1021 tccttgccct ggccgagctg gtcgccctcg taggtgtacc aggcgccggc cttgcggacg
     1081 agcccggtct ccacgccgac gtcgatcaat ccgccttcgc gactgatgcc ctggccgtac
     1141 atgatgtcga actctgcctg cttgaacggg ctggcgacct tgttcttcac gaccttcacc
     1201 cgggtgcggt tgccgacgaa gtcggtgccg tccttgaggc tctcgatgcg ccgtacgtcc
     1261 agccgcaccg acgagtagaa cttcaacgcc cgcccacctg tggtggtctc ggggctgttg
     1321 tggaccatca cgccgtccac gaagtagttg tggttgccct cgacctcgat gtcgaacttg
     1381 tgcatcgaac gcgtcggcgg cttgatgtgg atgctcagga tccgcgccgg taccggacgg
     1441 acctctggct gcacgaaccc aggctgcacg tcgaactggc cgcgctggtt ttcgaacagc
     1501 ttgtggtcca tcgacgggtg gatgtacgga gccaccaggt cgaggaaccg gcgggtcgac
     1561 tgcgtagcga aggtcagcac cgccttgcgg ttggcacctg ccagtctcca ggacacgtcg
     1621 aatccgtagg cgtcgcgcag gaacgcgacc agccggtcgc gggatccttc tgacatcgcc
     1681 tccacaccga acgccacccg tccgctaccg tcgcgggtcc gctgttgcaa ccctttggag
     1741 cgcacgctca gcgatccgtc gtccatgtac cagatcgcca gcgccaacgg tgtcagcgcc
     1801 ttcaggcaat cctcggtgat cgtcttcttg ccgtcaccca tgtagacggt gcgctggagc
     1861 tctccgagct ccggcaacgg agtgaaatcc acgaaactcg cacgctcggt atcgcgacga
     1921 cttgaccgga tgttgccgag cagtgagacc ttccagtcga ggtaggcacg ctgcctgaca
     1981 ccgtgaccga ggcggaagcg cactccgttg cggtctcgca ggttgggcga aagattgcca
     2041 tcaccgagta gcgagcccaa gacgacctgc cactgcagcg ggctcaaccg gtgagtctcg
     2101 gacgccatca cccgatcacc ggggatgagc tcgccagctt ggcgccagcc ctgaggtgtc
     2161 cggatgaggt ggttctccgt caccccgaac tgcaaccgtc cgttcccatg cgacttctcg
     2221 actgtgaact gcaggaagcg atccgcgatg ccgttgtcga accagttgac gaccttccgc
     2281 ggcacgacct tgtccgtctc cgggtcgtag gagagcacct cgacgtccat cttctggttg
     2341 acgatcttgc gaatcttctc cgtcgtgccg tcggccaaca ccacccgagc gtctcccgag
     2401 aaacaaccga acatgacgcc gatcttttcg cggagctggt tgatgaagat cgcggtcgtg
     2461 ccggagttgc tcaacgcgcc ggtgagcttg cgcagtgcct ggctcatcag gcgtgcctgc
     2521 agcccgacgt gcgagtcgcc catctctcct tcgatctcgg cgcgcggcac gagcgcggcg
     2581 acggagtcga tgacgatgat gtcgagggcg ccggaccgga tcagcatgtc ggcgatctcg
     2641 agcgcctgct cgccgctgtc gggctgggag accagcagcg cgtcggtgtc gacaccgagt
     2701 ttctgggcgt actccggatc gagcgcatgc tcggcatcga tgaacgccgc gatcccgccg
     2761 ttgcgctgcg cgttggccac cgcgtgcagc gccaccgtag ttttgccgct gctctccggt
     2821 ccgaatatct cgaccacgcg accacggggc aggccgccga tacccaaggc gatgtcgagc
     2881 gagatggccc cggtggggat cacctccacc ggagcgtggg actggtcgcc gagccgcatg
     2941 accgacccct tgccgtgggc ccggtcgatc tgcgccagtg cgttgtccag tgccttcatg
     3001 cggtctgcat cagccatctc agaagccatc cctgtgctcg ggtcggatgt ggtcgggtcg
     3061 ggtctcacga cgagcccggg gtgtgcgggt cgagcaggac gctagaggtg gccaccgaca
     3121 gtgccgccga agccggtggt cctgtggagg aacgccctcg tctgcgcgtc gtgtggacca
     3181 cgctatctag aacaggtgtt cgaacggcgg tgcgacacac cgtacgctga cgtgttccgc
     3241 gaattgcggg gaatgtcatc tcggcaccgg cgtgtcaacc tgcactttcc cgcaattcgc
     3301 gggaagccac gggcgacggt cagcgctccc ggggagggag ctcgagcacc ttccacacct
     3361 cgcgccagac ggtcttggcg tcatacccct cgtcgaacgc ctcgacgacc gtgcgtccac
     3421 ccaatccctc gatgacatgc tcgcgcgccc agacacgggc ataggtcgac ccgagtgccg
     3481 cctccatccg cgaccagaac tccgtgaccc gcacgtccag cctccgcctc cgccttctcg
     3541 ccaggcggtc agggtacgcc gacgcggtca gaggttcagc cgatccttca acacggcgta
     3601 cccgacgaac gcaacgacat cgaggatcgt gtgcgcaacg atgagcggca ggactcgccc
     3661 gcggcgtatg aagaacgtgc cgaacagcag acccatcacg gcgttgccga ggaatgcgcc
     3721 gaacccctgg tagaggtggt agctgccgcg cagcacggca ctcgctgcgg tggcccacac
     3781 cggcgtccag ccgagctgtc gcagtctcgt gatcaggtag ccgacgacga cgacttcttc
     3841 cagcaccgcg ttctgcgccg cggacaagac gagcaccggc agcgtccacc agtggtcgtt
     3901 gagcgccgac ggcacgatgg tggcgttcag gtcgagcgcc ttggccaggt agtacagccc
     3961 gagccccggg atgccgatca gcgcagccag gccggctccc caagccaggt cgcgcccgag
     4021 cccggcccga ctgcgtacgt cgacgcccaa ctgcctgatc gccgacgcgc cgcccgcgct
     4081 gaggaggaag atcaccagcg ccaccggcat caacgcgaag aagatgccgg ccagctggta
     4141 agtgagatcc agcagcggcc gtggcgtcac gctctggttc agggtcgcgg tctgctccga
     4201 gagcctgccc ggctcggtca gcttgccgac caggctgatc acggcgtacg tcgccgacgc
     4261 cccgagcgac agccccagca cgatgacgat ctcccagctg agccgccgcc gggccgtcgt
     4321 tgcgtcctgg acgaccactg cccccgctcc gtccacgtgg ctggatgcga gacgaggttg
     4381 aacttctggc tggcttgtca cgctgtcagt gtgccgcttg cggccaagag cgtgctgaga
     4441 tcccaagagc aggctgagag aggagaagtt gggactcctc aggtttctgc ggggcggaga
     4501 cggtcgtggg tgggcgtggt cgctcggctt ccgtcgtatg agctcgggtg ggtcggcaac
     4561 gaggagcggt aaccgttcgt gccccgctga ggccggtcgg tcggcgcccc cgtccctagt
     4621 gcctggtgtg tgactggcgg gtttcggata tccgcggttg accggacccg gtttgcctcg
     4681 gtccccacgc agacccaccg ctcccaacgc agatctgcct ggctacccgt ccggttggat
     4741 ggctacccag gcaaaccacc accgacaccc tgtcccattg gggcggtaga tggctttccg
     4801 gagacggtgg ccgcgccgat gcaagggcac ggacgaagtc ctgccgtgca gagcctgccc
     4861 gaaccggtgc atcgaggctc acgagtcaag ggcgatggac ggacccgagc gacgcagtcg
     4921 ccgggtccgg ctcaacaagc cacctgcgcc tgaccgaagc cgtcgcgaag cgatcgcgac
     4981 agcgaccctt gacgcgtgag gaatgcaccg gtagcaggcg cggc
//
LOCUS       DATBFZ010000521         2773 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_6166852, whole genome shotgun sequence.
ACCESSION   DATBFZ010000521 DATBFZ010000000
VERSION     DATBFZ010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2773
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6166852"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="VH419_16105"
     CDS             <1..273
                     /locus_tag="VH419_16105"
                     /inference="COORDINATES: protein motif:HMM:NF026049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7045193.1"
                     /translation="ERGMPFREAHALVGALVRDSIERRVPLAELVASHPDLGEDALEL
                     LEPGVAVTRRTTPGGAGPESVAIQIDRYRTLLEKLDTKLSLSPPKA"
     gene            270..653
                     /locus_tag="VH419_16110"
     CDS             270..653
                     /locus_tag="VH419_16110"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEY7045194.1"
                     /translation="MSFRHDIRVRYGEVDMQGVVFNAHYLAYVDDCIDSWLRSIEAHF
                     EQFGWDFMVKKATVEWLGTAGLGDVLTLVPRVSRWGRTSFDVTVVGAVDERPVFEAVV
                     VYVGVETGTLTPAPVPDEIRTALSA"
     gene            650..1237
                     /locus_tag="VH419_16115"
     CDS             650..1237
                     /locus_tag="VH419_16115"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF002003.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEY7045195.1"
                     /translation="MTSSILDRAFYGRDPRHVAPDLLNKVLVRGELSARIVEVEAYCG
                     SIDPGSHAYRGRTNRNATMFGPPGGLYVYFTYGMHWCANAVCGDDDEGVAVLLRAAAP
                     LTGIEQMRTARGAAARRDRDLCSGPAKLCEAFALDKAFDGADLVTADRGVTIVDDGTR
                     PPPQPGNSRRIGLTAGAEHPWRWFVAGDVNLSRRQ"
     gene            complement(1245..1763)
                     /locus_tag="VH419_16120"
     CDS             complement(1245..1763)
                     /locus_tag="VH419_16120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7045196.1"
                     /translation="MSQTSFLQTRTIRILVGIVIGVLAVMWIYVLFIAKPVTPDRLSD
                     RSFPTAAEPICAATITTMKNAGLVNQQASSPQDRAQLADRADAQLTDMVAQLRTKVPA
                     TGEAHDAVSKWLDDWDQWLRDRAAWTAKLRAGEDVQFLEKQRDSGEPNSQALNAFAVT
                     NDMGSCQTPAGV"
     gene            complement(1876..>2773)
                     /locus_tag="VH419_16125"
     CDS             complement(1876..>2773)
                     /locus_tag="VH419_16125"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEY7045197.1"
                     /translation="TNLIEGTVHSFNTLFAQLILQVGPKNAIDMAHRMGITTPLQAVP
                     AAVLGSNDVQPLEMASVYGTLANRGVHVDPVMITRITKANGTVLYEDVHEQKKAMDET
                     VADTVTTVLQQVIARGTGTAAQLDRPAAGKTGTGEDYKNAWFCGYTPTLSTAVWVGFP
                     ETEVQMTPPATSITVYGGTWPAQIWQRFMTAASAGTPPADFAPPAPATTTTTTTPPPA
                     TTAPPTTTATQVTASLDVPVPSVVGMAADQATSQLSQSGFVVERVDKGQAGTAGVVKS
                     QSPRPGTMAASGASVTIVVAPQ"
BASE COUNT          402 a          946 c          932 g          493 t
ORIGIN      
        1 gagcggggga tgccgttccg ggaggcgcac gccctcgtcg gtgcgctcgt ccgtgactcg
       61 atcgaacggc gcgttccgct ggccgagctc gtcgcctcgc atcccgatct cggcgaggac
      121 gcgctggagt tgctcgaacc cggtgtggcc gtcacgcggc gtaccacgcc cggcggtgcc
      181 ggccccgagt cggtcgcgat ccagatcgac cggtaccgca ccctgctcga gaagctcgac
      241 acgaagctgt cgctgtcgcc gccgaaggca tgagcttccg ccacgacatt cgggtccggt
      301 acggcgaggt cgacatgcag ggcgtggtct tcaatgcgca ctatctcgcg tacgtcgacg
      361 actgcatcga ctcgtggctc cggtcgatcg aggcccactt cgaacagttc ggctgggact
      421 tcatggtcaa gaaggccacg gtcgagtggc tgggcacggc aggtctcggt gacgtgctga
      481 cgctcgtgcc tcgcgtctcg cgctggggcc gcacgtcctt cgatgtcacc gtcgtcggtg
      541 ccgtggatga gcgtcccgtg ttcgaagccg tcgtcgtcta cgtcggcgtc gagaccggaa
      601 cgctgacccc ggcacccgtt cccgacgaga tccgtaccgc gctctcggcg tgacgtcttc
      661 gatactcgac cgggccttct acgggcgcga cccaagacac gtcgcgccgg acctgctcaa
      721 caaggtgctg gtccgaggtg agctgtcggc tcgcatcgtc gaggtggagg cgtactgcgg
      781 ctcgatcgat cctgggagcc acgcataccg cgggaggacg aaccgcaacg cgaccatgtt
      841 cggcccaccg ggcggtctct acgtgtactt cacctacggc atgcactggt gcgcgaatgc
      901 agtgtgcggc gatgacgacg agggtgtcgc cgtgctcctg cgagcagccg cgccgctcac
      961 cggcatcgaa cagatgcgaa cggcccgagg tgcagccgct cgccgcgacc gcgacctctg
     1021 cagcggcccg gccaagttgt gtgaggcgtt cgctctcgac aaggccttcg acggcgcgga
     1081 tctcgtgacc gccgaccgcg gcgtcacgat cgtcgacgac ggcacccgtc caccaccgca
     1141 gcccggcaac agccgccgca tcggcctcac cgctggcgcg gagcacccct ggaggtggtt
     1201 cgtggcgggt gatgtgaacc tgtcgcggcg tcagtgagga cgcgtcagac gccggccggc
     1261 gtctggcatg accccatgtc gttcgtcacc gcgaacgcgt tgagcgcctg gctgttgggc
     1321 tcgcccgagt cccgctgttt ctcgaggaac tgaacgtcct caccggcccg gagcttggcc
     1381 gtccacgccg cccgatcacg caaccattgg tcccagtcgt cgagccactt cgacaccgca
     1441 tcgtgtgcct cgcccgtggc cggcaccttg gtgcggagtt gcgccaccat gtccgtcagt
     1501 tgcgcgtcag cccggtcagc gagttgcgcg cgatcctgcg gtgacgacgc ctgctggttg
     1561 accagccccg cgttcttcat ggtggtaatg gtcgccgcac agatcggctc ggccgccgtc
     1621 ggaaacgatc gatcgctgag gcgatcaggt gtgaccggct tggcgatgaa aagcacatag
     1681 atccacatca cagcgagcac gccgatgacg atcccgacga ggatccggat cgtgcgcgtc
     1741 tgcaggaacg acgtttgact cattgcggcg ccaccacatc tctgtggggg cttcgccccc
     1801 acaccccagc gcatccggct ccctggcggt cgctcggctg cggtggtggc gccggtgccc
     1861 gattggtgcg cttgctcatt gcggcgccac cacgatcgtc accgaggctc ctgaggctgc
     1921 catcgtgccg ggccgcggtg actgcgactt cacgacgcca gcggtgccgg cctgtccctt
     1981 gtccacccgc tcgaccacga accccgactg ggacagctga ctcgtcgcct ggtccgccgc
     2041 catgccgacg acggagggca cgggcacgtc gagcgaagcg gtgacctgcg tcgcggtcgt
     2101 cgtcggcggt gcggtcgtcg ccgggggcgg cgtcgtcgta gtggtggtgg ttgccggtgc
     2161 aggcggggcg aagtcggcgg gaggcgttcc agcgctcgcc gccgtcatga accgctgcca
     2221 gatctgcgcc ggccacgtgc cgccgtagac ggtgatgctg gtggcgggcg gagtcatctg
     2281 cacctcggtc tcgggaaagc caacccacac ggcagtcgac aacgtcggtg tatagccgca
     2341 gaaccacgcg ttcttgtagt cctccccggt cccggtcttg cccgcagccg ggcggtcgag
     2401 ttgagcggcc gttcccgtgc cgcgggcgat gacctgctgc agaacggtcg tgacggtgtc
     2461 ggccacggtc tcgtccatcg ccttcttctg ctcgtgcacg tcttcgtaca gaaccgtccc
     2521 gttcgccttc gtgatccggg tgatcatcac cggatcgacg tgcacgcctc ggttggcgag
     2581 ggtgccgtag accgacgcca tctcgagtgg ctgcacgtca ttcgagccga gcactgccgc
     2641 cggcaccgct tgcagcggcg tggtgatgcc catccggtgg gccatgtcga tcgcattctt
     2701 cggcccgacc tggaggatca actgtgcgaa cagcgtgttg aacgagtgga ccgttccctc
     2761 gatcaggttc gtg
//
LOCUS       DATBFZ010000522         3282 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_6446506, whole genome shotgun sequence.
ACCESSION   DATBFZ010000522 DATBFZ010000000
VERSION     DATBFZ010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 3282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3282
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6446506"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="VH419_16130"
     CDS             complement(<1..277)
                     /locus_tag="VH419_16130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7045198.1"
                     /translation="MTSNGEEPEPLTDDEPDENEDVGPDNRIRLAVGLVALEALALVV
                     FAGIAVATIDPDRKGNGIVTVVFLLLYAAGLSLCALGLYRLRSWSRGP"
     gene            681..929
                     /locus_tag="VH419_16135"
     CDS             681..929
                     /locus_tag="VH419_16135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013160928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HEY7045199.1"
                     /translation="MDWRHRAACLDEDPELFFPIGNTGPAILQIEEAKAVCRRCDVRE
                     QCLAWALEAGQDHGVWGGMSEDERRALKRRNARAKIRA"
     gene            complement(1028..2488)
                     /locus_tag="VH419_16140"
     CDS             complement(1028..2488)
                     /locus_tag="VH419_16140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEY7045200.1"
                     /translation="MPSLNDLVTAHTDLDPDDVAWLHLLVADWQIIADLSFADLVLWL
                     PDRDGIGFWGGAQMRPTTGPTAFVDDLVGAFVPRGRRPLLDTAYDERRIAREGDPEWR
                     DDVPVRVETIPVLRAGALLGVIARNTNLLGVRTPSRLELAYLQTASDLTRMISQGSFP
                     VPAERREWTSSPRVGDGFVRTDAAGVVTYASPNAQSAYRRLGLATDLVGEAFAPTTAV
                     LVNPAVQAVDESLSVTLSGLHHREVEYANDDATVAARVIPLTPDGRHEGAAILLRDVT
                     ELRSRERELITKDATIREIHHRVKNNLQTVAALLRLQSRRMDVSAARLALDEAVRRVG
                     SIAIVHETLSQTFDERVEFDEVADRLAAMVTEVAAEPAVVATTRTGSFGQLPAEAATP
                     LAMVLTEVMQNAVEHAFPEGSGRISLRVLRLAGRLEVTVDDDGTGLPADFDPVSSGNL
                     GLSIVRTLVESELGGVITLGTNPEGGTRVDLDIPLA"
     gene            2577..>3282
                     /locus_tag="VH419_16145"
     CDS             2577..>3282
                     /locus_tag="VH419_16145"
                     /inference="COORDINATES: protein motif:HMM:NF022431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2785 domain-containing protein"
                     /protein_id="HEY7045201.1"
                     /translation="MPRGFWQQVLESDMAVPDNPPLNELTAELVTMLGSTNPVDRDET
                     AYSVLAAWIGEGVYDDLLVSVGDSIAQGLRIGVGTSLDDTVFRRSFSGLVLAECVRRD
                     NVARILPVDVVLNWAERAIGWYTREQDLRGWIAGKGWAYAVAHGADLIGALGSSRHLQ
                     TQHLGVLLDVIAERVTSPTPTVLVDGEDDRLALAALTIVHRNLVDTETLEAWVATMAQ
                     ALTRPRDHLTEHTWPTP"
BASE COUNT          499 a         1168 c         1117 g          498 t
ORIGIN      
        1 ccgggccacg gctccagctg cgcaaccggt acagcccgag ggcgcagagg ctgagtccgg
       61 cggcgtagag caggaggaac accacggtga cgatgccgtt gcccttgcgg tcggggtcga
      121 tcgtcgccac cgcgatcccc gcgaagacga ccagggcgag cgcctcgagg gcgaccagtc
      181 cgaccgccag cctgatccgg ttgtcgggcc cgacgtcctc gttctcgtcc ggctcgtcat
      241 ccgtcagcgg ctcgggttcc tcgccgttgc tggtcatgac cgacacccta gggccgcgac
      301 cggcgtacgc cgcatcggtc ccggacgtgc cgaccggcgg ggcaccgcac ccgaggcaga
      361 cgtgaccgtg gccacgatcg gggatcgtca ctcccggtcg cgctcgcgcg tgcctagact
      421 ggatcgcggg atgtggacgc atgtctttct cgtccggatt gtgaggaaac tgacaaccgt
      481 cgaggggttg tgtcgcgccg catctcttgc gacgatcgag tcgcgctctg tcacgacgct
      541 gtggcgtcgg acgctctcac ctaccccgca gccagccgag cgaggcgggt gagacggttc
      601 gtgaaaacgt tcacgagccc cagaccacga tgtggtcgcg gtaccccgcc gcggccacca
      661 ggatgaagag gagccgcgcc gtggattggc gtcaccgtgc cgcgtgcctc gacgaggacc
      721 cggagctgtt cttccccatc ggcaacacgg gcccggccat cctccagatc gaggaggcca
      781 aggcggtgtg ccgtcgatgc gacgtacgcg agcagtgtct cgcctgggcg ctcgaggccg
      841 gccaggacca cggtgtctgg ggcgggatgt ccgaagacga gcggcgcgcc ctgaagcggc
      901 ggaacgcccg cgccaagatc cgcgcctgac cactcctgtg ggcctccgcg aattgcggga
      961 agctccggct tttccggcgg tagattcccg gaacctcccg caattcgcgg ggagcccggc
     1021 acccgggtca ggcgaggggg atgtcgaggt cgacgcgagt gccgccttcc gggttggtgc
     1081 cgagggtgat caccccgcca agctccgact cgacgagcgt gcgcacgatc gagagcccga
     1141 ggttccccga cgacacgggg tcgaagtccg ccggtagccc cgtgccgtcg tcgtcgaccg
     1201 tgacctccaa gcggcccgcc agccggagca cacgcaacga gatccggccc gagccttccg
     1261 ggaaggcgtg ctcgacggcg ttctgcatca cttccgtcag gaccattgcc aacggtgtgg
     1321 cggcctccgc gggcagctga ccgaacgacc cggtacgagt cgtggccacg actgcgggtt
     1381 ctgccgccac ctcggtgacc attgcggcca acctgtcggc gacctcgtcg aactcgaccc
     1441 gctcgtcgaa ggtctggctc aacgtctcgt gcacgatcgc gatggagccc acccggcgta
     1501 ctgcttcgtc gagcgccagt cgcgcagcgg acacgtccat ccgacgagac tggagtcgca
     1561 gcagcgccgc gacggtctgc aggttgttct tgacccgatg gtgaatctcc cggatcgtcg
     1621 cgtccttcgt gatcagctcg cgctcgcggc tgcgcagctc ggtcacgtca cggagcagga
     1681 tggccgcccc ttcatgccgg ccgtccggcg tcagcgggat gacgcgcgcc gcgaccgtgg
     1741 cgtcgtcgtt ggcgtactcg acctcccgat ggtgcagccc ggacaacgtg accgacagcg
     1801 actcgtcgac ggcttggacc gccgggttga ccagcaccgc ggtcgtcggc gcgaacgcct
     1861 cccccaccag atcggtcgcc aacccgagac gccggtacgc cgactgggcg ttcggcgacg
     1921 cataggtcac cacgccggct gcgtcggtcc ggacgaaacc gtcgccgacc cgcggtgagc
     1981 tggtccactc acgtcgctcc gccggcaccg ggaacgaccc ttggctgatc atcctcgtca
     2041 gatcgctggc ggtctggagg tacgccagct ccaaccggct cggcgtccgg actccgagca
     2101 ggttcgtgtt gcgcgcgatc acgccgagca gtgcgccggc ccgcagcacc gggatcgtct
     2161 cgacccgcac gggaacgtcg tcgcgccact ccgggtcgcc ctcgcgcgcg atccgtcgtt
     2221 cgtcgtacgc cgtgtcgagc agcgggcggc gaccacgcgg cacgaacgcc cccaccaggt
     2281 cgtcgacgaa ggcggtcggg cccgtcgtcg gccgcatctg tgctccgccc cagaagccga
     2341 tgccgtcccg atcgggcagc cacagcacca agtcggcgaa cgacagatcg gcgatgatct
     2401 gccagtcggc cacgagcaga tgcagccacg cgacgtcgtc cgggtcgagg tccgtgtgcg
     2461 ccgtgacgag gtcgttcagc gagggcacgt gagcaacggt agggcctggg cacccgtcgg
     2521 tgcagcgctg tccgacgggg agtccgcgcc ccgaaccgtg tggaaggatg ggcgccatgc
     2581 cacgcggttt ctggcagcag gtgctcgagt ccgacatggc ggttcccgac aacccgccgc
     2641 tcaacgagct caccgcagag ctcgtcacca tgctgggttc gaccaacccc gtcgaccgcg
     2701 atgagaccgc gtactcggtg ctcgccgcct ggatcggtga gggcgtgtac gacgacctgc
     2761 tcgtcagcgt cggcgactcc atcgctcaag gcctccgcat cggtgtgggc acgtcgctgg
     2821 acgacacggt gttccgtcgc agcttctcgg gactggttct ggccgagtgc gtccggcggg
     2881 acaacgtggc ccgcatcctg cccgtcgacg tcgtcctcaa ctgggccgaa cgtgccatcg
     2941 gctggtacac ccgcgagcag gacctgcgtg gttggatcgc cggcaagggc tgggcgtatg
     3001 ccgttgccca cggcgccgac ctgatcggcg cactcgggtc gtctcgccac ctgcaaaccc
     3061 agcacctcgg tgtgctgctg gacgtgatcg ccgagcgcgt gacctcgccg acgccgaccg
     3121 tgctcgtcga cggggaggac gaccggctcg cgctggcggc gctgacgatc gtgcaccgta
     3181 acctcgtcga caccgagacg ctcgaggcct gggtggcgac catggcgcag gcgctcaccc
     3241 gaccgcgcga tcacctcacc gagcacacct ggccgacccc gg
//
LOCUS       DATBFZ010000523         1501 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_4528063, whole genome shotgun sequence.
ACCESSION   DATBFZ010000523 DATBFZ010000000
VERSION     DATBFZ010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4528063"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            284..493
                     /locus_tag="VH419_16150"
     CDS             284..493
                     /locus_tag="VH419_16150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003949541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BldC family transcriptional regulator"
                     /protein_id="HEY7045202.1"
                     /translation="MASRTHEPEPLLTPAEVASMFRVDPKTVTRWAKAGKLSAIRTLG
                     GHRRYRESEVRALLQGQIPAQRQGD"
     gene            complement(527..838)
                     /locus_tag="VH419_16155"
     CDS             complement(527..838)
                     /locus_tag="VH419_16155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7045203.1"
                     /translation="MSALSSHFAARQGGVMTDEVGTVTGDLTLRTEVDEDGTLRVLVQ
                     YLGADEWYRITDAPTSLPADTSLASVHARLLDELRVSQPRVTSTDDDGPLTGLGGGRA
                     E"
     gene            complement(835..>1501)
                     /locus_tag="VH419_16160"
     CDS             complement(835..>1501)
                     /locus_tag="VH419_16160"
                     /inference="COORDINATES: protein motif:HMM:NF019151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HNH endonuclease family protein"
                     /protein_id="HEY7045204.1"
                     /translation="LVQTPNVSAVLRRLATAVLAGLLGITGLAACGPIDESLGQPGPF
                     DSGEVSAELDGLTVAGWASMTGYSRDRFPTWDSQGQGCDTRDRVLQRDGRNVVTGDDC
                     KILNGAWTSAYDGEQVDDPSKLDIDHVVPLADAWRTGAAAWTDEKREAFANDLTRPQL
                     VAVSASSNRSKGDQDPSQWRPPRHDYWCTYAESWVTVKAYWHLSVTTDEKAALVELLG
                     TCP"
BASE COUNT          247 a          509 c          508 g          237 t
ORIGIN      
        1 gcgaacggtg acgcggagac ggctaccgcg tcgtacgagc ggctcgtgga acggtggcgc
       61 gcgatccagc tgctggagcg ctgcaactag agtccgcgaa cgttgtaatt gaaccgttac
      121 cgttcaaagc ggacaatcgg cgtgtaactc gtccgaccat ccacaaccca tcatccgttc
      181 ggcccatgtc ggacatcggg gactagccgg acgatgagta gcgctggcgc gctggggccc
      241 tcaccaaccg cccgccacga gtacctggta gaggagatac ccgatggcat cccgtacgca
      301 cgagccagag ccattgctta ccccggctga ggtcgcgtcg atgttccgcg tcgacccgaa
      361 gacggtgacc cggtgggcaa aggccggcaa gctcagtgcc attcgtactc tcggtggaca
      421 ccgacggtac cgcgagtccg aggtacgcgc cctgctccag gggcagatcc cggcgcagcg
      481 acagggcgac tgagatccgt acggcgatca ttccatagga actgacctat tcggcccgcc
      541 ctccaccaag cccggtgagc gggccgtcgt catcggtgga cgtgacccgc ggctggctca
      601 cgcgcagctc gtcgaggagc cgcgcatgga ccgaagccag gctcgtatcg gccggtagcg
      661 atgtcggcgc gtccgtgatc cggtaccact cgtcggcgcc caggtactgc acgagcaccc
      721 gcagggtgcc gtcctcatcg acctccgtcc gcagcgtgag gtcgccggtg accgtgccga
      781 cctcgtcggt catcacgcca ccctgcctcg ccgcgaagtg gctgctcaga gcgctcacgg
      841 gcaggtcccg agcagctcga ccagggccgc cttctcatcg gtcgtgaccg acaggtgcca
      901 gtaggccttg acggtgaccc aggactcggc atacgtgcac cagtagtcgt gccggggtgg
      961 ccgccactgg gatgggtcct ggtcgccctt ggaacggttg ctggatgcgc tgaccgcaac
     1021 caactggggt cgcgtgaggt cgttggcgaa cgcctcacgc ttctcgtcgg tccaggcggc
     1081 ggcaccggtc cgccaggcgt cagccagcgg cacgacgtgg tcgatgtcca gcttgctggg
     1141 gtcgtcgacc tgctcgccgt cgtatgcgct ggtccacgct ccattgagga tcttgcaatc
     1201 gtcaccggtg acgacgttgc ggccgtcgcg ctggagcacc cggtcgcgcg tgtcacagcc
     1261 ctgcccctgc gagtcccagg tgggaaagcg gtcgcggctg taaccggtca tcgaagccca
     1321 cccggccacc gtcaacccgt cgagctcggc actgacctcg ccgctgtcga acggcccggg
     1381 ctggccgaga gactcgtcga tgggcccgca cgcggccagg cccgtgatgc cgagcagccc
     1441 ggcgaggacg gcggtggcga ggcgacgcag gacagcggag acgttggggg tctgcacgag
     1501 c
//
LOCUS       DATBFZ010000524         1635 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_4858606, whole genome shotgun sequence.
ACCESSION   DATBFZ010000524 DATBFZ010000000
VERSION     DATBFZ010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 1635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1635
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4858606"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            204..1529
                     /locus_tag="VH419_16165"
     CDS             204..1529
                     /locus_tag="VH419_16165"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HEY7045205.1"
                     /translation="MTDTSTADATDVDTPRADTLTIRDNRSGREYEVPITDGTIRAAD
                     LKQIAVEDDPGLATYDPGFVNTASCRSSITYIDGDVGILEYRGYPIEQLAENSTYLEV
                     AYLLLNGELPTSDQLDAWVHEVTYHTFLHENLKQFIQGFRYDAHPMGMLMAAVSALST
                     FYPEARNVLDPEVRALQITRMIAKMPTLGAFAFRHGQGRPYVYPDNDLSYTENFLAML
                     FKMSEPKYDADPRLVKALEILFILHADHEQNCSTNAVRSVGSSQVDPYSAVAAGIGAL
                     YGPLHGGANEAVLRMLREVKTVDRIPEFISGVKEGKEKLMGFGHRVYKNYDPRAKIIK
                     KACDDVFDVTGVNPLLKIAQELEKIALEDEYFVKRRLYPNVDFYSGLIYEALGFSPEM
                     FTVLFAIPRTSGWLAQWQELITDKEQKIARPKQIYTGHRSRDFVPIDQR"
BASE COUNT          302 a          549 c          506 g          278 t
ORIGIN      
        1 gacggtcgac atcgtctccg aaccgcgggt ttcacccgcc ttgtatcgtc gtccactgct
       61 cgccggctgt gggtccgtcg gtaggggcga aacggtcgaa agcgagcctg ctggactccc
      121 gacgcccgcg gtttacgccc agttcgggtg ggagcatctc acccgactgg tccgcgccgc
      181 tgctgcccgc gtagtttgtg cgcatgacag atacgtccac ggcggatgcg accgacgtcg
      241 acaccccccg cgcagacacg ctgacgatcc gcgacaaccg gagcgggcga gaatatgagg
      301 tgccgatcac cgacggcacc atccgggccg ccgatctgaa gcagatcgcc gtcgaggacg
      361 acccgggcct ggcgacgtac gatccgggct tcgtcaacac cgcctcatgc cgcagctcga
      421 tcacctacat cgacggcgat gtcggcatcc tggagtatcg cggctatccg atcgagcagc
      481 tggccgagaa ctcgacctac ctcgaggtgg cctatctgct gctcaacggc gagctgccca
      541 cctccgacca gctcgacgcg tgggtgcacg aggtcaccta ccacaccttc ctgcacgaga
      601 acctcaagca gttcatccag ggcttccgct acgacgcgca cccgatgggc atgctgatgg
      661 ctgccgtcag cgcgctctcc accttctacc ccgaggcgcg taacgtgctc gaccccgagg
      721 tgcgcgcgct gcagatcacc cggatgatcg ccaagatgcc gacgctcggc gcgttcgcct
      781 tccggcacgg tcagggacgg ccgtacgtct atcccgacaa cgacctgtcc tacaccgaga
      841 acttcctcgc gatgttgttc aagatgagcg agccgaagta cgacgcggac ccgaggctgg
      901 tcaaggcgct cgagatcttg ttcatcctgc acgccgacca cgagcagaac tgctcgacca
      961 atgcggtccg gtcggtgggc tcgtcgcagg tcgatccgta ctcggcggtt gccgcgggta
     1021 tcggcgccct ctacggcccg ctgcacggcg gggccaacga ggccgtgctg cggatgctgc
     1081 gcgaggtcaa gacggtcgac cggatccccg agttcatctc cggtgtcaaa gagggcaaag
     1141 agaagctgat gggcttcggt caccgggtct acaagaacta cgaccctcgg gccaagatca
     1201 tcaagaaggc gtgcgacgac gtcttcgatg tcaccggagt caacccgctg ctgaagatcg
     1261 cgcaggagct ggagaagatc gcgctggagg acgagtactt cgtcaagcgg cggctgtacc
     1321 ccaacgtcga cttctactcc ggactcatct acgaggcgct gggcttctcc cccgagatgt
     1381 tcacggtgct gttcgcgatc ccgcgcacct cgggctggct cgcgcagtgg caggagctga
     1441 tcaccgacaa ggagcagaag atcgcccggc cgaagcagat ctacaccgga catcgcagcc
     1501 gggacttcgt gccgatcgac cagcgctgaa cctcggtcgc gaaccgattt gggcaattcc
     1561 tgacattcgt ttggcggacg cccgcccgtt cacgcctcag gcgtgtgagc cgtcgcgcga
     1621 cgagtgtcag gaatt
//
LOCUS       DATBFZ010000525         3631 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_5787688, whole genome shotgun sequence.
ACCESSION   DATBFZ010000525 DATBFZ010000000
VERSION     DATBFZ010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 3631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3631
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5787688"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..532)
                     /locus_tag="VH419_16170"
     CDS             complement(<1..532)
                     /locus_tag="VH419_16170"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEY7045206.1"
                     /translation="MTDSHAERRAALIAGALALPFALLTVLVVAEWGPLMRLDAHVVD
                     SWHGAVVGTPWESFFIGVARISQPWVDRIVLLVLAVVLALRQQTRTALWILTVVVVSG
                     PAYRLVQHLVARPRPDVPHQIGGFSFPSGHATQIAATMGVLVVLALQRVRRRRLRRVL
                     TAAAVLVVGLVGVDRIF"
     gene            671..1966
                     /gene="serS"
                     /locus_tag="VH419_16175"
     CDS             671..1966
                     /gene="serS"
                     /locus_tag="VH419_16175"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017933443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEY7045207.1"
                     /translation="MIDLRVLRQDPELARESQRRRGASPELVDEILERDEARRRSIAT
                     FEKLRAEQKAIGKEVAKAQGEEKATLLSRTKELSQQVKAAEAAQSEAEAAFDEAMALM
                     PNLAAPEAPAGGEDDYVVLEHIGAPRDFEAEGFEPLDHVELGRRLGAIDVERGAKVSG
                     ARFYYLTGVGAELEFALVNMAMDQARDAGFTPMIPPALVKPRAMEGTGFLGQAAGDVY
                     RLPEEDLYLVGTSEVPLAAYHSDEILEAETLPRRYVAFSPCYRKEAGSYGKDTRGIIR
                     VHWFDKVEMFTYTTLEDSYKEHECLLNWEKEWLDKLELSYRVIDTAAGDLGLSAQRKF
                     DCEAWIPTQGRYRELTSTSNCTEFQARRLNIRGRFPSTGSGQGSEIKPLATLNGTLVA
                     IPRTIVAILETHQQADGSVRVPAALQPYLQGRTTLEPIA"
     gene            2050..2847
                     /locus_tag="VH419_16180"
     CDS             2050..2847
                     /locus_tag="VH419_16180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011753671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HEY7045208.1"
                     /translation="MALDLDGTLVDFDDTARPLVVDAVQAVRDSGVHVVLATGRSVFG
                     ILPVIDAFGLTDGYGVASNGAVTFTYHPTTVRTAITFDPEPAVRAVLDHVPDALVAVE
                     VIGRGYRTNRHFPPGEINGEIWLETVDELVREPVTRVIIRDPQSSVDDFVALAEQLGL
                     HGTNYYVGYTAWLDLAPEGVSKASGLAGIAAELGVDRSDVLAIGDGRNDLEMLRWAGR
                     GVAMGHAPDVVKQAADAVTASWDDDGVAVELAAWFDGRIEVRAPERR"
     gene            2861..3454
                     /locus_tag="VH419_16185"
     CDS             2861..3454
                     /locus_tag="VH419_16185"
                     /inference="COORDINATES: protein motif:HMM:NF014520.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HEY7045209.1"
                     /translation="MTDFAAGAAPPGDEFTATPCATDLDLYLHPLLEDPPVRSTAAKE
                     TWTEYETLVNTARASCSTCPLLADCMYKAVVQTDVSGYVGCTTPKERAAIRKLLDVKV
                     EADDLDSFAGTRGTRQPVDHDDVLRMRAQHPDESLEALASRLGCSLSTVKRHLRRARR
                     EAAGPHVTIADTLPTMEEVFDAFDEVVEANRARSRVC"
BASE COUNT          617 a         1262 c         1243 g          509 t
ORIGIN      
        1 cgaagatccg atcgactccg acgaggccga cgacgagcac cgcagcagcc gtcaacacac
       61 gccggagccg tcgacgccgg acacgctgca gggccagcac caccaggacg cccatcgtcg
      121 cagcgatctg ggtggcatgc ccggacggaa acgagaagcc gccgatctgg tggggtacgt
      181 ccggccgtgg ccgcgcgacc agatgctgga cgagccggta cgccgggccg gacaccacca
      241 cgacggtgag gatccacaac gcggtacgcg tctgctgccg tagcgccagg acgacggcca
      301 gcaccagcag tacgatccgg tcgacccagg gctggctgat cctcgcgaca ccgatgaaga
      361 aggactccca cggcgtaccg acgaccgcgc cgtgccagga gtcgacgacg tgggcgtcga
      421 gccgcatcag cgggccccac tcggcgacga cgaggaccgt cagcagcgcg aacggcagcg
      481 cgagcgcacc ggcaatcagc gctgcgcgcc gttcggcatg cgagtcggtc actggactct
      541 ctcgggaagt ctcggttcgc gttcggccgc cgtcacgcgg cccgcaggtc agccgacagt
      601 atgccgtgag ccggcactcg gccagaaaag gcgagtgagg tcgtcaccca ccgctggata
      661 cccttgcgga gtgatcgatc tgcgtgtact ccgccaagac ccggagctgg cccgcgagtc
      721 gcagcggcgg cggggcgcat cgccggagct ggtcgacgag atcctggagc gggacgaggc
      781 gcgcagacgg tccatcgcca ccttcgagaa gctccgtgcc gagcagaagg cgatcggcaa
      841 ggaggtcgcg aaagcacaag gcgaggagaa ggccaccctg ctgtcccgca ccaaggagct
      901 ctcgcagcag gtcaaggcgg ccgaggcagc ccagagcgag gccgaggcag cgttcgacga
      961 ggcgatggcc ctgatgccca acttggccgc cccggaggct ccggccggtg gcgaggacga
     1021 ctacgtcgtg ctcgagcaca tcggtgcgcc acgtgatttc gaggcggagg gcttcgagcc
     1081 gctcgaccat gtcgagctgg ggcggcggct cggcgccatc gacgtggagc gtggcgccaa
     1141 ggtctccggg gcccgcttct actacctcac cggcgtcggg gccgaactgg agttcgcgct
     1201 cgtcaacatg gcgatggacc aggcacgcga cgccggcttc acgccgatga tcccgcctgc
     1261 gctggtgaag ccacgcgcga tggagggcac gggcttcctc ggccaggccg ccggggacgt
     1321 gtaccggcta cctgaggagg acctgtacct ggtcggcacc tccgaggtgc cgctggcggc
     1381 gtaccactcc gacgagatcc tcgaggccga gacgttgccg cggcggtacg tcgcgttcag
     1441 cccgtgctac cgcaaggagg ccggctcgta cggcaaggac acccgcggca tcatccgggt
     1501 gcactggttc gacaaggtcg agatgttcac ctacacgacg ttggaggact cctacaagga
     1561 gcacgagtgc ctgctcaact gggagaagga gtggctggac aagctcgagc tgtcgtaccg
     1621 cgtcatcgac accgccgccg gtgacctcgg cctgtccgcg cagcgcaagt tcgactgcga
     1681 ggcctggatc ccgacgcagg gccgctaccg ggagctgacg tccacgtcga actgcacgga
     1741 gttccaggcc cggcgcctca acatccgcgg ccggtttccc tcgacaggct cagggcaagg
     1801 aagtgagatc aagccgctgg cgacgctgaa cggcacgctg gtcgcgatac cacggaccat
     1861 cgtggcgatc ctcgagacgc atcagcaggc cgacggctcg gtccgggtcc cggccgctct
     1921 ccagccgtac ctgcaaggtc gtacgacgct ggagcccatc gcgtgacccc acctcgtgcc
     1981 gtcggccgcg atcggccggg gcagcaggac gggcagcggc gaagcggcgc cggcacgagg
     2041 ccagcgctca tcgcgctcga cctcgacggg acgctggtgg atttcgacga cacggcccga
     2101 ccactggtcg tcgacgccgt ccaggccgtc cgagactccg gcgtccacgt cgtcctcgcc
     2161 accggccgct cggtcttcgg catcctgccg gtgatcgacg cgttcggact cacggacgga
     2221 tacggcgtcg cgagcaacgg cgcggtgacg ttcacctacc acccaacgac cgtccgtacg
     2281 gcgatcacgt tcgaccctga gcctgcggtc cgtgctgtcc tcgaccacgt gcccgacgca
     2341 ctggtggcgg tcgaggtgat cggccgcggc taccggacga accggcattt cccacccggc
     2401 gagatcaacg gtgagatctg gctcgagacc gtcgacgagc tggtccggga gccggtcacc
     2461 cgcgtcatca tccgcgaccc gcagtcgtcc gtcgacgact tcgttgccct ggccgagcag
     2521 ctgggacttc acggcaccaa ctactacgtg ggctacacgg cctggctgga cctcgcaccg
     2581 gaaggcgtgt cgaaggcgtc tgggctcgcc ggcatcgccg cggagctcgg tgtcgaccga
     2641 tccgacgtgc tcgcgatcgg agacggccgc aacgaccttg agatgcttcg ctgggctgga
     2701 cgcggcgtgg ccatgggaca cgcccctgac gtggtcaagc aggcggccga tgctgtgaca
     2761 gcgagctggg acgacgacgg ggtcgccgtc gagttggccg cctggttcga tgggcgtatc
     2821 gaggttcgcg caccagaacg acgatgacgg gggtcatccg atgaccgact tcgccgcggg
     2881 ggcggcacca cccggcgacg agttcacggc aaccccctgc gcgacagacc tggatctgta
     2941 cctgcatccg ctgctcgagg accctccggt ccgctccacg gctgccaagg agacctggac
     3001 ggagtacgag acgctggtca acaccgcccg cgcatcgtgc tcgacgtgcc cgttgctcgc
     3061 ggactgcatg tacaaggccg tggtccagac cgacgtcagc gggtacgtcg gatgtacgac
     3121 gccgaaggag cgggcggcga tccgcaagct gctcgacgtc aaggtcgagg ccgacgacct
     3181 ggactcgttc gccggtacgc gtggaacgcg gcagcccgtc gaccacgacg acgtgctgcg
     3241 gatgcgcgcc caacacccgg acgagtcgtt ggaggccctc gcgagccggc tcggctgctc
     3301 cctgtcgacg gtgaagcggc accttcgccg ggcgcggcgc gaggcggccg gcccgcacgt
     3361 gacgatcgcg gataccctgc cgacgatgga ggaggtcttc gacgccttcg acgaggttgt
     3421 cgaggccaac cgcgcccgct cccgcgtctg ctgaccgaca cgggctcgac gtcctaactc
     3481 gcctgactcg gcctagtacc ccgtcaacca acattcgccc tgttgcgcgc cgcccgggcg
     3541 gcgatctgcg gcgttatcgt cggtcaacga tgctgcgcat cgcctccctc ctctgccttg
     3601 cagctcatcc acccggacac cgctcacgac g
//
LOCUS       DATBFZ010000526         2614 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_4861354, whole genome shotgun sequence.
ACCESSION   DATBFZ010000526 DATBFZ010000000
VERSION     DATBFZ010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2614
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4861354"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1258
                     /locus_tag="VH419_16190"
     CDS             <1..1258
                     /locus_tag="VH419_16190"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY7045210.1"
                     /translation="SVGRARPRSSYGHGIQYVDASDDTVIPGLWESHAHEGMDQPFAG
                     GRKNRLELSLGVTTEMSMGDEPYHSLEEVESQQSGAAPGPRYFWSGEPIDGGRIFYSW
                     MRPDPTAASMTTNLSRLAKLKPDILKTYVRLPNDMEQAAIQAGHDLGIPSFSHYFWPA
                     LAFGQDGTSHWATQRLGYQIAVSQDNVAYDDTIQLYARSGMSITTTPFAGATTFLPNI
                     NGQPTINDPRMKALLSPWNYALAQQEFAQPPLTAAQLSTSVGFGKAVAKIVAAGGTTL
                     VGTDNPIGYGNFGQVIAISAMAHTGLSNYEALRGATVEPAKVMGVLDQIGTIQPGMIA
                     DLDIIHGNPLEDIQTIANDVYVMQNGNLYTPQELIGPYGAGNPAQDATKLAPARKTPG
                     QRAPAWQTLGELQKRTSFDYIMEYMC"
     gene            1296..1751
                     /locus_tag="VH419_16195"
     CDS             1296..1751
                     /locus_tag="VH419_16195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7045211.1"
                     /translation="MRTVSSTYTPAREAEETASRAALGVASVALAGMAYCHIRDVGMK
                     FDEHVYYMAGLFCGNIALSLALIPALILAELRGSARSRQIVWAATAGLAAATIVGFVW
                     SRTIGFPQMADHVGEWDALGVTSLVFEGILLALSLAMLTHVARTKMHRP"
     gene            complement(1800..>2614)
                     /locus_tag="VH419_16200"
     CDS             complement(1800..>2614)
                     /locus_tag="VH419_16200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7045212.1"
                     /translation="ARSIGEILKITTPGANTVKWLAVDIKGNQEAVQSKSFLLDQTAP
                     TVTVNIPEGAVYTQGRPVPLTFSCADDQGGSGIASCVGSTPSGSNLPTGTAGMHVLTI
                     TATDNVGNVFVKTVNYRVLDATNVNGTVGGNVPATLSLTLGAPAAFGQFTPGIDNDYT
                     ASSTASVLSTAGDATLSVSDPSSTNTGQLVNGTFALPSKLTVSAASAGSTAGAGGPVG
                     GSSAPTTLSTWSNPVSNDVVTITFKQHVSRTDALRTGTYAKTLTFTLSTTTP"
BASE COUNT          438 a          856 c          906 g          414 t
ORIGIN      
        1 gagcgtcggc cgggcgcgcc cgcggtcgtc ctacggtcac ggcatccagt acgtcgatgc
       61 gtccgatgac acggtgattc cgggtctgtg ggaatcgcat gcgcacgagg ggatggacca
      121 gcccttcgcc ggcggccgga agaaccgcct cgagctctcg ctcggggtca ccaccgagat
      181 gtcgatgggc gacgagccgt accactcact cgaggaagtc gagtcccagc agtcgggcgc
      241 ggcgcccgga ccgcggtact tctggtccgg cgaaccgatc gacggaggcc ggatcttcta
      301 ctcctggatg cgacctgacc cgaccgccgc gtcgatgacg acgaatctgt cgcggctcgc
      361 gaagctcaag ccggacatcc tcaagaccta cgtccggctg cccaacgaca tggagcaggc
      421 cgcgatccag gcgggccacg acctcgggat cccgtccttc tcgcactact tctggccggc
      481 gctggccttc ggccaggacg gcacctcgca ctgggcgaca cagcgcctgg gctaccagat
      541 cgccgtgtcc caggacaacg tcgcctacga cgacacgatc cagctctacg cccgttcggg
      601 catgtcgatc acgactacgc cgtttgcggg cgcgacgacc ttcctgccga acatcaatgg
      661 gcagccgacc atcaacgacc cgcggatgaa ggcactgctg agcccgtgga actacgcgct
      721 ggcacagcag gagttcgcgc agcctccgct caccgcggct cagctgtcga cctcggtcgg
      781 gttcggcaag gcggtggcga agatcgtcgc ggcgggtggc acgacgctgg tcggtaccga
      841 taatcccatc ggctacggga atttcggaca ggtcatcgcc atcagcgcga tggcacatac
      901 cggcctcagc aactacgagg ccctgcgtgg agccacggtc gagcccgcca aggtcatggg
      961 cgtgttggat cagatcggca ccatccagcc gggaatgatc gccgacctgg acatcatcca
     1021 cggcaacccg ctggaggaca tccagacgat tgccaacgac gtgtacgtga tgcagaacgg
     1081 caacctgtac accccgcagg agctgatcgg cccgtatggg gccggcaacc cggcacagga
     1141 tgccaccaag ctcgcacccg cgaggaagac acccgggcag cgcgctcccg cgtggcagac
     1201 gctcggcgag ctgcagaagc ggacgagctt cgactacatc atggaataca tgtgctagcc
     1261 ggttcgggag tcggcgaccg ccgaggaggt cagtggtgag gaccgtgagt agtacctaca
     1321 cacccgcacg cgaagcggag gagacggcaa gccgagcggc gctcggggtg gcctcggtcg
     1381 cgctcgccgg aatggcctac tgccatatcc gcgacgtcgg catgaagttc gacgagcacg
     1441 tgtattacat ggcagggctc ttctgcggca acatcgccct gtcgctcgcg ctcatcccgg
     1501 cgttgatcct ggccgaactg agaggatcgg cgaggtcgag gcagatcgtg tgggccgcga
     1561 ccgcgggact ggcggcggcg accatcgtgg gtttcgtgtg gagtcgcacg atcggattcc
     1621 cccagatggc cgaccacgtc ggtgaatggg acgccctggg agtgacctcg ctggtcttcg
     1681 agggcatcct gctcgccctc agcctcgcca tgctcacgca cgtcgcgcgg acgaagatgc
     1741 atcgcccgtg aaacgtctgg ccccggagcg cgcggacgcg ctccggggcc gatcggacgc
     1801 tacggggtgg tcgtggacag cgtgaacgtc agcgtcttcg cgtacgtgcc ggtacggagg
     1861 gcatccgtgc ggctcacgtg ctgcttgaac gtgatggtca cgacgtcgtt ggagaccggg
     1921 ttgctccacg tggagagcgt ggtgggagcg gaggagccgc ccaccgggcc gcccgcaccg
     1981 gccgtgctgc cggcgctcgc cgccgagacg gtcagcttgc tgggcagcgc gaacgtgccg
     2041 ttcaccagct ggccggtgtt ggtcgagctc gggtcggaga cgctcagcgt cgcgtcaccg
     2101 gcggtcgaga gcacgctggc cgtcgacgac gcggtgtagt cgttgtcgat gcccggcgtg
     2161 aactggccga acgcggccgg cgcgcccagc gtcagagaca gcgtggcggg cacgttgccg
     2221 ccgacggtgc cgttgacgtt ggtggcgtcc agcacgcggt agttgacggt cttgacgaac
     2281 acgttgccga cgttgtcggt cgcggtgatc gtcagcacgt gcatgccggc ggtgccggtc
     2341 ggcaggttgg agccgctcgg agtcgagccg acgcacgagg cgatgcccga gccaccctgg
     2401 tcatccgcgc acgagaacgt gagcgggacc ggacggccct gcgtgtagac cgcgccctcc
     2461 gggatgttga ccgtgacggt cggagccgtc tggtcgagca ggaacgactt gctctggacg
     2521 gcctcctggt tgcccttgat gtcgaccgcg agccacttga cggtgttcgc gcccggcgtg
     2581 gtgatcttga ggatctcgcc gatgctccgc gccc
//
LOCUS       DATBFZ010000527         5122 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_4531643, whole genome shotgun sequence.
ACCESSION   DATBFZ010000527 DATBFZ010000000
VERSION     DATBFZ010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 5122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5122
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4531643"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..758)
                     /locus_tag="VH419_16205"
     CDS             complement(<1..758)
                     /locus_tag="VH419_16205"
                     /inference="COORDINATES: protein motif:HMM:NF016143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HEY7045213.1"
                     /translation="MRFRDNARLDTGEVSDQRGGIRPGLMIGGGGGLIGVIIAVVIAL
                     SGLGGGGGSANNVIDTGSGGSDLATECKTGSDANQRTDCLVVGVVDSVQEYWAGAING
                     YQPARTVLFTGQVGTGCGTATSDVGPFYCPNDERVYLDLGFYQELHDRFGARGGPFAE
                     AYVIAHEYGHHVQDLLGTSSTAAARGEGPLGGSVRLELQADCYAGVWAAHAVQTSQIS
                     EITDQDIADGLDAAAAVGDDRIQQAATGHVNQET"
     gene            911..1159
                     /locus_tag="VH419_16210"
     CDS             911..1159
                     /locus_tag="VH419_16210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7045214.1"
                     /translation="MPQRWEYMTLDLNKRRTELDSLNELGRDGWEAVAMVSSWGVGWR
                     WVHPIVLLKRPVRGDGTVGESDGRSDSRHDTGLTTSTA"
     gene            1229..3583
                     /locus_tag="VH419_16215"
     CDS             1229..3583
                     /locus_tag="VH419_16215"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEY7045215.1"
                     /translation="MTSASDPPATGLTTAEVQARIADGRTNRVPQATSRPLSAIIRAN
                     VLTRFNGILGVLVVAVVSVGSPGDALFGLVLVSNSLIGIVQELRAKRTLDRLAVLTAP
                     GVTVRRDGVPVTVALEDVVLDDLVVLRAGDQVPADGEVVQSDELEVDESLLTGEAEPI
                     AKSASSQVLSGSIVVAGSGAFRATAVGINAYAQRLTAETRRFTTARSELMTSINALLR
                     FISWGLLVIGPLLLASQLVRGSESASDAVVSTVAALVGMVPEGLVLLTSIAFFVGALA
                     LARRSVLVRELPAVEGLARVDVVCFDKTGTITDGEIRFAAIDPIVQDADTSAALGALG
                     DDPDANSTLRALAAAFPPPDGWRGQASVPFSSARKFSARTFEGRGTWILGAPEVLLNP
                     ATASDVSDRVAGLAASGRRVVLLARSDRAARAPDLLAHVDPVALVSLEEGIRPDSADA
                     VRYFKTQNVDLKVMSGDNAVTVAAVAREVGIPRAETPVDARLLPALDADWEPIVEERA
                     VFGRVTPSQKRAFVGALQRRNHVVAMTGDGVNDALALKDADVGVAMGSGAPATRAVAQ
                     LVLLDSRFAHVPQVVAEGRRVIANIERVANLFLIKNVYAALLALVVIVIGLPYPFLPR
                     QLTVVSTLTIGIPAFVLSLAPNETRYRPGFLGRVVRFSVPAGVITAIGVMTTYALARA
                     QHRTPVEARAAATVVAMIVGLRVLLLVARPLRPWKVALVLTMGAAFALIMVVPALRDA
                     FKLNLPASMLAQSLLIGALAALALGLADRGLRQDESGTGNVAQS"
     gene            complement(3612..4481)
                     /locus_tag="VH419_16220"
     CDS             complement(3612..4481)
                     /locus_tag="VH419_16220"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY7045216.1"
                     /translation="MDRAPGIIVGVDGSACADMALRWAVDEARLRSAALVAVLVWGWL
                     NQHPLPGGPEFDPSYDAAAARQALDAFVDRAVGPDANEVGRRVVNDLPARALLVEARD
                     ADLLVVGARGLGGLRGLLLGSVSQRCAHLAMVPVAIVREGSESRTGPVVVGVDGSGAS
                     CEALGWAADEACRRGSQLEVVHAWRDGTAWLPRLLPLHDSRVAESEAARLVAGLIDER
                     VEGDLCPGVVISSTGAAAALVEASATASLLVVGNHGHGTVGGTLLGSVAQQVAHHAAC
                     PLVLVPASRVGRI"
     gene            complement(4644..>5122)
                     /locus_tag="VH419_16225"
     CDS             complement(4644..>5122)
                     /locus_tag="VH419_16225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY7045217.1"
                     /translation="SYYTLRRANCDPKPLQDPHPPIWIGGGGEQLTLRVVARYADCSN
                     FGGRPDEWAHKCEVLKGHCAAVGRDYDEIRKTWSPELLLRETEAEVVEAGSRSIWGEA
                     FDRWRESNLVGTPEQVAEKIKTYVDLGCRGVVPWCADYPDTQSLELFASKVMPAFA"
BASE COUNT          766 a         1786 c         1707 g          863 t
ORIGIN      
        1 cacgtctcct ggttcacgtg gccggtcgcg gcctgctgga tgcggtcgtc gccgaccgcc
       61 gccgcagcgt cgaggccgtc ggcgatgtcc tgatccgtga tctcgctgat ctggctggtc
      121 tggacggcgt gggccgccca cacacccgca tagcaatcgg cctgtagttc gagtcggacc
      181 gaaccgccga gcggcccttc gccccgggcc gccgcggtcg agctggtgcc gaggagatcc
      241 tggacatggt gtccgtactc gtgggcgatc acgtaggcct cggcgaaggg accgccgcgg
      301 gcgccgaacc gatcgtggag ctcctggtag aagccgagat cgaggtacac ccgctcgtcg
      361 ttcgggcagt agaacggccc gacgtcggac gtcgcggtcc cacagccggt gccgacctgg
      421 ccagtgaaca gcacggtgcg cgccggctgg taaccgttga tcgcgccagc ccagtactcc
      481 tgcaccgagt cgacgacgcc cacgacgagg cagtcggtgc gctggttcgc atcggatccg
      541 gtcttgcact ccgtggccag gtcggaccct cccgacccgg tgtcgatgac gttgttcgcc
      601 gacccacccc cgcccccgag gccggagagc gcgatcacga cggcgatgat cacgccgatc
      661 agaccgccgc caccgccgat catcagcccg ggacggattc caccgcgctg gtccgatacc
      721 tccccggtgt cgaggcgtgc gttgtccctg aacctcatcg atcgctctcc gctctcagcc
      781 gaagcgctgg tgtacccacg ctaggtgtgg ggcaatcgtc gacgaccgac cgcgggagtc
      841 gtgacctttg gccctgggcg ggcacgccgt cgtggttcac gctgacagct ggccgaacgg
      901 aggcactgac atgccgcagc gttgggagta catgacgttg gacctcaaca agcggaggac
      961 cgagctcgac agcctcaacg agctcgggcg cgacggctgg gaggccgtgg ccatggtgtc
     1021 gtcatggggc gtcggttggc ggtgggtgca cccgatcgtt ctgctgaagc gaccggttcg
     1081 aggtgacggc acggtcggcg agagcgatgg tcggtccgac tcacggcacg acacagggtt
     1141 gacgacgtcg actgcgtgac gtcggtttcg taccgatcgt ccgcgggcca tcaaggtccc
     1201 ggcgcgacgc cggttagcgt cggtcgtagt gacgtcggct tcagacccgc cggcaacggg
     1261 cctcacgacg gccgaggtcc aggcgcgtat cgcggacgga cggacgaacc gggttccaca
     1321 agcgaccagc cgtccgctct cggcgatcat ccgcgccaat gtgctgaccc gtttcaacgg
     1381 catcctcggc gtactcgtcg tcgccgttgt atcggtcggg tcgccgggcg acgcattgtt
     1441 cgggctcgtg ctcgtctcga actcgctcat cggcatcgtg caggagctga gagccaagcg
     1501 cacgctcgat cgcctggcgg tcctgaccgc gccgggagtg actgttcggc gcgacggagt
     1561 cccggtcacc gtcgcgctcg aagacgtcgt gctcgacgac cttgtcgtgt tgcgggccgg
     1621 tgatcaggtt ccggctgacg gcgaggtcgt ccagagcgac gagctcgagg ttgacgagtc
     1681 gctcctcacc ggtgaggcag agccgattgc gaagtccgca agctcgcagg tcctctccgg
     1741 ctcgatcgtc gtggccgggt caggtgcctt ccgtgcgacc gcagtcggca tcaacgctta
     1801 tgcccagcgt ctcaccgccg aaacgcgacg cttcacgacg gcccgctccg agctcatgac
     1861 cagcatcaac gcgctgctcc ggtttatcag ctggggtctt ctcgtcatcg gtcccttgct
     1921 gcttgccagc cagctcgttc gaggatcgga gagcgcgagc gatgcagttg tcagcaccgt
     1981 cgctgcgttg gtgggcatgg tcccggaggg gctcgtcctg ttgacgagca tcgcgttctt
     2041 cgtgggtgcg ctcgcgctcg cgaggcgttc ggtcctcgtt cgcgagctcc ccgcggtcga
     2101 aggattggcg cgtgtcgatg tcgtgtgctt cgacaagacg ggcacgatca ccgatggaga
     2161 gatccgcttc gcggcgatcg acccgatcgt ccaggacgcc gacacctcgg ccgcgctcgg
     2221 cgcgctcggc gacgatcccg acgccaactc gaccttgcgg gcgctcgcgg cggcgttccc
     2281 gcctccagac ggatggcgag gccaagcctc agtcccgttc tcctctgccc gcaagttcag
     2341 tgcgaggacc ttcgagggcc gtggtacctg gatcttgggc gcaccagagg tcctgttgaa
     2401 cccggccact gcttctgacg tcagcgatcg cgtcgcgggc ttggcggcct cggggcgtcg
     2461 ggtcgtgctg ctcgctcgat ccgatcgcgc tgcccgcgcg ccagacctcc ttgcccacgt
     2521 cgacccggtg gccttggtca gtctcgagga aggcatccgg ccagactctg ccgacgccgt
     2581 gcgctacttc aagactcaga acgtcgacct gaaggtcatg tcgggcgaca acgccgtcac
     2641 cgtcgctgcc gtcgcgcgcg aggtggggat cccaagagct gagacacctg tcgacgctcg
     2701 actgctgcca gcactcgacg ccgattggga gccgatcgtc gaagaacggg cggtgttcgg
     2761 ccgggtcacg cccagtcaga agcgcgcgtt cgtcggtgcg ctccagcggc gcaaccatgt
     2821 cgtcgccatg accggagacg gcgtaaacga cgccctggcg ctcaaagatg ccgacgtcgg
     2881 cgtcgcgatg ggaagtggcg caccggcgac ccgcgccgtc gctcagctcg tgctcctcga
     2941 cagccggttc gcgcatgttc cccaagtggt tgcagaaggc cggcgggtca tcgcgaacat
     3001 cgagcgggtc gccaacctgt tcttgatcaa gaacgtctat gcggcgcttc tcgccctcgt
     3061 tgtgattgtg atcggtttgc cgtatccgtt tctccctcgc cagctgacgg tcgtcagtac
     3121 gttgaccatc ggcattcccg cgttcgtgct ctccctcgca ccgaacgaga cccgctaccg
     3181 gcccggcttt ctcggccgag tcgtgcgctt ctccgtccca gctggtgtga tcacggcgat
     3241 cggcgtgatg accacgtacg cacttgctcg cgcccagcat cggacgccag tcgaggcgag
     3301 ggctgcggca accgtcgtgg cgatgatcgt cggactgcgg gtgctgttac tcgtcgcccg
     3361 cccactgcga ccgtggaagg tcgccctcgt gctgacgatg ggtgctgcgt tcgctctcat
     3421 catggtcgtg ccagcgctcc gcgacgcgtt caagctgaat ctcccggcgt cgatgctggc
     3481 gcagtcgctg ctcatcgggg cgcttgccgc gctcgcgctc ggtctcgccg accgtgggct
     3541 gcgtcaagac gagtccggca caggcaacgt tgcgcagagt tgagggcgca cccggtctcg
     3601 gccgttgacg ttcagatgcg tccgacgcgt gaggcaggca cgagaaccag cggacacgcc
     3661 gcgtggtgcg cgacctgctg cgcgacggac ccgagcagtg tcccgcccac cgtaccgtgg
     3721 ccgtggttcc ccacgacgag gaggctggcc gtcgcggacg cctcaacgag cgcagccgcc
     3781 gcgccggtgc tcgagatcac gacccctggg cacaggtcgc cctcgactcg ctcgtcgatc
     3841 aggccggcga cgaggcgagc cgcctcggac tcggcgaccc gactgtcgtg gagtggcagc
     3901 agtctgggca gccaggccgt tccgtctcgc cacgcgtgca ccacctcgag ttgagatccg
     3961 cgtcggcagg cctcgtcggc ggcccacccg agcgcctcac aggatgcccc ggagccgtcg
     4021 accccgacga cgacgggacc ggttcgagat tcactacctt cacgaacgat ggcgacggga
     4081 accatcgcga ggtgggcgca gcgctgactc accgagccga gcagcaggcc acgtagaccg
     4141 cccagcccac gcgccccaac gacgaggagg tccgcgtccc tggcctcgac gagcagcgct
     4201 cgtgccggca gatcgttgac cactcgcctg ccgacttcgt tcgcgtccgg gccgaccgcc
     4261 cggtcgacga aggcatcaag cgcctgacgt gccgccgcgg cgtcgtacga cgggtcgaat
     4321 tcgggaccgc cgggcagtgg gtgctggttc agccagcccc ataccagcac cgcgacaagg
     4381 gccgcgctgc gaagtcgggc ctcgtcgacg gcccagcgca gtgccatatc agcacacgcc
     4441 gacccatcga cgccgacgat gatgcctggg gcgcgatcca cgatctcatc gtcgcctcgg
     4501 cgcggcggcg tgcaccgagg cggaggtccc gaccggccgg gccgacagac ccgttccaaa
     4561 ccaacttgca ctcggcggaa cgttctcgcg ccctccggga ccttcgcccc ttgggtcacg
     4621 ggcggatcgg gcgcccgcgt gactcaggcg aacgcgggca tgaccttcga cgcgaagagc
     4681 tccaagctct gcgtatccgg ataatcggca caccacggga cgacgccgcg gcagccgagg
     4741 tcgacgtacg tcttgatctt ctcggccacc tgctcgggcg tgccgacgag gttgctttcc
     4801 cgccagcggt cgaacgcctc gccccagatg ctgcgactgc cggcctccac gacctcggcc
     4861 tccgtctcgc gcagcaggag ctcgggtgac cacgtcttgc ggatctcgtc gtagtcgcgc
     4921 ccgaccgccg cgcagtgacc cttgagcacc tcgcacttgt gcgcccactc gtcgggccgc
     4981 ccaccgaagt tggagcagtc ggcgtagcgc gccaccaccc gcagggtcag ctgctcaccg
     5041 cctccgccga tccagatcgg cggatgcgga tcctgcaacg gcttcggatc gcagttggcg
     5101 cgtcgcaacg tgtagtagct gc
//
LOCUS       DATBFZ010000528         2298 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_4533197, whole genome shotgun sequence.
ACCESSION   DATBFZ010000528 DATBFZ010000000
VERSION     DATBFZ010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2298
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4533197"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            69..1292
                     /locus_tag="VH419_16230"
     CDS             69..1292
                     /locus_tag="VH419_16230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7045218.1"
                     /translation="MSKVVYLHVGLHKTGTTYLQDYFSSNREGLVSQGVDFPSGDGEP
                     VQVLAAYDLRGRRARGYTDKRVAGHWAALTEHIRDSPAPVALVSQESLALATLKEARR
                     AVASFPDLEVHVVVTARDLGRTLVSMWQEEIKAEGTWTWREYADAVKDADRATSNPAF
                     GFWRREDLIRVCDTWAAAATLDHLHIVTVPPSGTDHSELVRRVASVVGFDPTALTEPP
                     RRSNEMIGVPGIEVLRQVNVRLGDRLNERARARAVRGELLSMLARPDSQRRAGLPESE
                     FDWVSKRADSMVETVRSRGFHVVGDLDDLRPRREDSARSPDDATDAELFEAAVDALAQ
                     LTERYGRSWWSKRRSRFEDVSEGGTLRSRLRGRTYKLKVKMLATADRSKLAAKAVGVA
                     VDAEARKLRRARNRG"
     gene            1325..>2298
                     /locus_tag="VH419_16235"
     CDS             1325..>2298
                     /locus_tag="VH419_16235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7045219.1"
                     /translation="MASEPKRIFLHIGLHKTGTTYLQNLMRVNRAPLCAAGVHYPGAT
                     GEPAPTRATRDLLRRSPRGYADPRIGGSWDALVATVRQQDLPTVVISDEGLGGCRRGH
                     VRRIVESFADREVHAVLTVRDLARVVVSEWQEEIKNDRSWTWQDFVDAVRDEDATFGN
                     GRAFWRRQDLVRKGEVWLTSLPAAHVHIVTVPKPGSPDDELVERFMRVVNIDRTHLPN
                     EPEWNNESVGVAGTEVIRRMNERMGGRLNEPAYNRAVKATLVPLLAQHTKPARFTLPV
                     EELGWVSARADQMIEWIKTNRLPVSGDLEDLRVSVDHHQGRRPDDVTK"
BASE COUNT          403 a          760 c          808 g          327 t
ORIGIN      
        1 tcgatcacgc ctgctgacgc ggtgccagcg gccgctgctg cccattggag tcaccgtagg
       61 atcagcccgt gtcgaaagtg gtctacctgc acgtcggtct gcacaagacg gggaccacct
      121 atctccagga ctacttcagc agcaaccgag agggactcgt ctcgcaaggg gtggacttcc
      181 ccagcggtga cggcgagccg gtccaggtgc tggcggcgta cgacctgcgt ggacgacgtg
      241 cccgcggcta caccgacaag cgggtcgccg gtcactgggc ggcgctgacc gagcacatcc
      301 gggactctcc ggcgccggtc gccctggtgt cccaggaaag cctcgcgctc gcaacgctga
      361 aggaggcacg tcgggcggtc gcgtcgttcc ccgacctcga ggtccacgtt gtcgtgaccg
      421 ctcgggacct gggccgcaca ctcgtgtcga tgtggcagga ggagatcaag gcggagggca
      481 cgtggacctg gcgcgagtac gccgatgcgg tcaaggacgc cgaccgggcg accagtaacc
      541 ccgccttcgg gttctggcgc cgggaggatc tgatccgtgt ctgtgacacg tgggctgcgg
      601 ccgccacgct ggaccacctg cacatcgtca ccgtgccacc atcgggcacc gaccactcgg
      661 agctggtgag aagggtcgcg tcggtggtcg gattcgaccc caccgcgctc accgaaccac
      721 cgcgccgctc caacgagatg atcggagtcc cggggatcga ggtgttgcga caggtcaacg
      781 tgcgcctggg tgaccgactc aacgagcgag cgcgagcccg cgccgtgcga ggggagctcc
      841 tctcgatgtt ggcgcgacct gactcacaac gacgggctgg gctgccggag tccgagttcg
      901 actgggtgtc gaagcgcgcg gactcgatgg tcgaaaccgt gcgttcgcgc gggttccacg
      961 tcgtcggtga cctcgacgac cttcgtccgc gacgcgagga cagcgctcgc tcacctgacg
     1021 acgccaccga cgcggagctc ttcgaggccg ctgtggacgc gcttgcgcag ctcaccgagc
     1081 gctacgggcg gtcctggtgg agcaagcgac ggtcacggtt cgaggacgtc agcgagggcg
     1141 gaaccctccg gagccggctg cgcggacgta cgtacaaact gaaggtgaag atgctggcga
     1201 cggccgaccg aagcaagctc gccgcgaagg cggtgggcgt cgctgtcgac gcggaggccc
     1261 gcaagctccg gcgggcacgc aaccgcgggt agcgtcagcc tgccgtcgac ggaaggaaag
     1321 ccagatggcg tcggagccga agaggatctt cctgcacatc ggcctgcaca agaccggtac
     1381 gacgtacctg cagaacctca tgcgagtcaa ccgagcgcca ctgtgcgcgg cgggagtcca
     1441 ctatcccggc gccaccggag agccggcgcc gacacgcgcc acccgggacc tgctcagacg
     1501 gtcgccgcgc ggctacgccg acccccggat cggcggctcc tgggacgcac tcgtcgccac
     1561 cgtccgccag caggaccttc ccaccgtggt gatctccgac gaagggctcg gcggatgtcg
     1621 gcgcgggcac gtgcgacgca tcgtcgaatc cttcgccgac cgtgaggtgc acgccgtgct
     1681 caccgtgcgc gatctcgccc gtgtcgtcgt gtcggagtgg caggaggaga tcaagaacga
     1741 ccgcagttgg acctggcagg acttcgtgga cgccgtacgc gacgaggacg cgacgttcgg
     1801 caacggtcgt gccttctggc gtcggcagga cctcgtacgc aagggtgagg tctggttgac
     1861 cagcctgccg gccgcgcacg tccacatcgt gaccgtgcca aagccagggt cgccggacga
     1921 cgaacttgtc gagcgcttca tgcgggtggt gaacatcgac cggacgcacc tgccgaacga
     1981 gccggagtgg aacaacgaga gcgttggcgt cgcgggcacc gaagtcatcc gccgcatgaa
     2041 cgagcgcatg ggcggaaggc tgaacgagcc cgcctacaac cgggcggtca aggccacgct
     2101 ggtgcccctg ctggcgcagc acacgaagcc tgctcgcttc acgctgccgg tcgaggaact
     2161 ggggtgggtg agtgcgcggg cggaccagat gatcgagtgg atcaagacga accggctgcc
     2221 ggtcagtggc gacctggagg acctacgagt ctccgtcgac caccaccaag gccggcgccc
     2281 ggacgacgtc accaagga
//
LOCUS       DATBFZ010000529         1505 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_3867066, whole genome shotgun sequence.
ACCESSION   DATBFZ010000529 DATBFZ010000000
VERSION     DATBFZ010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3867066"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="VH419_16240"
     CDS             <1..364
                     /locus_tag="VH419_16240"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEY7045220.1"
                     /translation="SLAFAAAAGVTGAVMLHAVAGTGARLGFLLGFLCFASHLVLDMF
                     GPDARPPYGIPLFWPLADHAFLSPVPLLLGVHHVSRTSASTREWVNAVLTLHNVAALG
                     LEIVLILPFLLLARRRRA"
     gene            500..>1505
                     /gene="asnB"
                     /locus_tag="VH419_16245"
     CDS             500..>1505
                     /gene="asnB"
                     /locus_tag="VH419_16245"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEY7045221.1"
                     /translation="MSDALRHRGPDDEGFYSDGVAALGHRRLSIIDLDGGHQPLTNED
                     GSLWIVFNGEIYNYRELRDRLVRDHRFATRSDTEVILHLYEERREKCVEELRGMFAFA
                     IWDTRDRSLFLARDHLGQKPLFYAHLDGRFLFGSEIKALLAADPSLREMDARALDEYL
                     ALRFISPPRTMFAKVRKLPPGCHMLYKNDRLTIQRYWAPIYQPKLVAPEADLLDELEQ
                     RVVETVRYHMVSDVPIGAFLSGGLDSSLIVALMSKLSPEPIKTFSVGVPYADYSELPF
                     ARLVAQACCTEHYEETINPSLIDTLPKLVWHMDEPSDPLSVCMYYIAAMARRHVKVVL
                     G"
BASE COUNT          235 a          511 c          446 g          313 t
ORIGIN      
        1 cagtctcgcc ttcgctgccg ccgccggtgt gactggtgcg gtgatgctgc acgccgtagc
       61 gggcaccggt gcccggttag gcttcctgct gggtttcctc tgctttgcgt cgcacctggt
      121 ccttgacatg ttcggtccgg atgctcgacc gccgtacggg atcccgctct tctggccact
      181 cgccgaccat gcattcctct cccccgtgcc actgctgctc ggcgtgcacc acgtcagtcg
      241 cacgtctgca tcaacgcgtg agtgggtgaa tgccgtcctg acgcttcaca acgtagctgc
      301 cctcgggctc gagatcgtgc ttatcctccc gttcttgctg ctggcccggc ggcgccgcgc
      361 gtagccagga cgatcgcgct acaatgtgtt tgttactcgc cacgcgtacg ggcgagggat
      421 aagtcgaggc tagccagtca tgtgtggaat ctgcggcatc gtggcgcccg gcggtgtaaa
      481 ccgtcgggcc gtcggcctga tgtccgacgc gctccgtcat cgtggtccgg acgacgaagg
      541 cttttattcg gacggcgtcg ctgcattggg gcatcgtcgg ctgtccatca tcgacttgga
      601 cggcggccat cagccgctca ctaacgagga tggttccctc tggatcgtct tcaacggcga
      661 gatctacaac taccgtgaat tgcgtgaccg tctcgtccgt gaccatcgct tcgcgacccg
      721 cagcgacacc gaggtcatcc tgcacctcta tgaggaacgc cgggagaagt gcgtcgagga
      781 gctgcgcggt atgttcgcgt tcgccatctg ggacacccgc gatcgttcgc tcttccttgc
      841 ccgcgaccac ctgggccaga agccgctctt ctatgctcac ctggacggcc gattcctgtt
      901 cggatcagaa atcaaggcgc tgctcgcggc cgacccgtcg ctgcgtgaga tggatgcccg
      961 agccctcgac gagtacctcg cgctccgctt catctcgccc ccgcggacca tgttcgcgaa
     1021 ggtccgaaag ctaccgccgg gctgccacat gctgtacaag aacgatcgtc tcaccatcca
     1081 gcgatactgg gccccgatct accagccgaa gctggtggcg cccgaggcgg acctgctgga
     1141 cgagctcgag cagcgtgtcg tcgaaacggt ccgctaccac atggtgagcg atgttcccat
     1201 cggggcgttc ctaagcggtg gcctcgactc cagcctcatc gtggccctga tgagcaagct
     1261 gtccccggag ccgatcaaga cgttctcggt cggcgttcct tacgctgact acagcgagtt
     1321 acccttcgcc cggctcgtcg cccaggcctg ctgcaccgag cactacgagg agacgatcaa
     1381 cccctcccta atcgacaccc tcccgaagct tgtctggcac atggacgagc cgtccgatcc
     1441 gctgtcggtt tgcatgtact acatcgccgc gatggcacgt cgtcacgtca aggtggtcct
     1501 cggcg
//
LOCUS       DATBFZ010000530         2077 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_4865385, whole genome shotgun sequence.
ACCESSION   DATBFZ010000530 DATBFZ010000000
VERSION     DATBFZ010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4865385"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2077
                     /gene="uvrA"
                     /locus_tag="VH419_16250"
     CDS             <1..>2077
                     /gene="uvrA"
                     /locus_tag="VH419_16250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009079664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEY7045222.1"
                     /translation="RPSSKQRITDSIETALALAGGLVVLDFVNLDEADPHRERRYSER
                     LACPNDHPLAIDELEPRSFSFNSPFGACPVCTGLGTRKEVDPELVVPDPELSLDEGAI
                     APWATGQTSEYFLRLLQGLAEAVGFDLDTPWDQLTARAQKAVLHGIDDQVHVSYRNRY
                     GRDRSYYAAYEGVVPWIERRYAETESDWSREKYEGYMREVPCPTCEGTRLKPEILAVT
                     VHGRSIAELAALSIGEANDWLQSLDLDERERLIGERVLKEIYARLGFLVDVGLHYLSL
                     DRASATLAGGEAQRIRLATQIGSGLVGVLYVLDEPSIGLHQRDNHRLIDTLVRLKELG
                     NTLIVVEHDEDTIRTADWVVDIGPRAGEHGGQIVVSGPVEELLTSQQSITGAYLSGRR
                     AIEVPPIRRPRQHDREVLVEGAREHNLRNIDVAFPLGCFVAITGVSGSGKSTLVNDIL
                     YAALANKINGAKLPPGRHKRVRGVEQLDKVVGVDQSPIGRTPRSNPATYTGVFDHIRK
                     LFSETTEARIRGYQQGRFSFNVKGGRCENCAGDGTIKIEMNFLPDVYVPCEVCKGARY
                     NRETLEVHYKGKTISEVLEMPIEEAAEFFKPISKIARHLDTLVDVGLGYVRLGQPAPT
                     LSGGEAQRVKLASELQKRQTGKTIYVLDEPTTGLHFEDVSKLLGVLHSLVDKGNTVLV
                     IEHNLDVIKT"
BASE COUNT          367 a          715 c          696 g          299 t
ORIGIN      
        1 ccgtccgtcg agcaaacaac gcatcaccga ttcgatcgag actgcgctgg ctctcgccgg
       61 cggcctggtg gtgctcgact tcgtcaacct ggacgaggcc gacccgcacc gcgagcgccg
      121 ctactccgag cggctggcct gccccaacga ccacccgctc gcgatcgacg agctcgagcc
      181 acggtccttc tcgttcaact cgccgttcgg cgcgtgcccg gtctgcaccg gcctcggcac
      241 gcgcaaggag gtcgatcccg agctcgtggt ccctgatccg gagctcagcc tcgacgaggg
      301 cgcgatcgcc ccgtgggcga ccgggcagac cagcgaatac ttcctgcggc tgctgcaagg
      361 cctcgccgag gcggtcggct tcgacctgga cacgccatgg gaccagttga ccgcgcgcgc
      421 gcagaaggcg gtgctgcacg gcatcgacga ccaggtgcac gtcagctacc gcaaccggta
      481 cggccgcgac cgctcgtact acgccgcgta cgagggcgtc gtgccgtgga tcgagcgtcg
      541 ctatgccgag acagagtcgg actggtcgcg cgaaaagtac gagggctaca tgcgcgaggt
      601 gccgtgcccg acgtgtgaag gcacccgact caagccggag atcctcgcag ttaccgtgca
      661 cggacgctcg atcgcggagc tcgccgcgct gtccatcggc gaggcgaacg actggctgca
      721 atcgctcgac ctcgacgagc gcgagcggct catcggcgag cgcgtgctca aggagatcta
      781 cgcccggctc gggttcctgg tggacgtcgg gctgcactac ctgtccctgg accgtgcctc
      841 cgcgaccctg gccgggggcg aggcgcaacg catccggctc gcaacgcaga tcgggtccgg
      901 cctggtcggc gtgctgtacg tgctggacga gccgtcaatc ggcctgcacc agcgcgacaa
      961 ccaccgcctg atcgacaccc tggtccggct gaaggagctc ggcaacacgc tgatcgtcgt
     1021 cgagcacgac gaggacacca tccgcaccgc ggactgggtg gtcgacatcg gtccgcgcgc
     1081 cggtgagcac ggcggccaga tcgtggtcag cggcccggtc gaggagcttc tcacctcgca
     1141 gcagtccatc accggcgcct atctgtcggg gcggcgcgcg atcgaggtcc cgcccatccg
     1201 gcggccgcga cagcacgacc gcgaggttct ggtcgagggc gcgcgcgagc acaacctgcg
     1261 caacatcgac gtggcgttcc cgctcggctg cttcgtcgcg atcaccggcg tgagtggttc
     1321 gggcaagtcg accttggtca acgacatcct gtacgccgcg ctggcgaaca agatcaacgg
     1381 ggcgaagctg ccgccgggac ggcacaagcg ggtgcgcggc gtcgagcaac tcgacaaggt
     1441 ggtcggtgtc gaccagtcgc cgatcgggcg caccccccgg tccaacccgg ccacgtacac
     1501 cggcgtgttc gaccacatcc gcaagctgtt cagcgagacc accgaggcga ggatccgcgg
     1561 ctaccagcag ggccgcttct cgttcaacgt caagggcggc cgctgcgaga actgcgccgg
     1621 cgacggcacc atcaagatcg agatgaactt cctgccggac gtgtacgtgc cctgcgaggt
     1681 ctgcaaaggc gcgcggtaca accgcgagac gctcgaggtg cactacaagg gcaagacgat
     1741 cagcgaagtc ctggagatgc cgatcgagga agccgccgag ttcttcaagc cgatctccaa
     1801 gatcgcccgc cacctggaca cgctggtgga cgtcgggctc ggctacgtcc ggctcggcca
     1861 gccggcaccg acgctgtccg ggggagaggc gcaacgggtg aagctcgcgt ccgagctgca
     1921 gaaacgccag accggaaaga cgatctacgt gctggacgag ccgaccaccg ggctgcattt
     1981 cgaggacgtg tcgaagctgc tcggcgtgct gcactcgctg gtcgacaagg gcaacaccgt
     2041 gctcgtcatc gagcacaacc tggacgtgat caagacc
//
LOCUS       DATBFZ010000531        10702 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_6177687, whole genome shotgun sequence.
ACCESSION   DATBFZ010000531 DATBFZ010000000
VERSION     DATBFZ010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 10702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10702
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6177687"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /locus_tag="VH419_16255"
     CDS             complement(<1..544)
                     /locus_tag="VH419_16255"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017934449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HEY7045223.1"
                     /translation="MPDARAEIAVIGGSGFYRFLDAPDDVTMATPYGEPSAPVSVGAV
                     HGRPVAFLPRHGRRHEFPAHQVNYRANLWALRSLGVRRVLAPCAVGSLQAELGPGTLV
                     VPDQLVDRTTNRVQTFYDVGACHVEFADPYCPSLRTALLGVDDTIVDGGAMVVVQGPR
                     FSTRAESQWYAAQGWSLVNMT"
     gene            complement(631..2811)
                     /locus_tag="VH419_16260"
     CDS             complement(631..2811)
                     /locus_tag="VH419_16260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020393377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEY7045224.1"
                     /translation="MARRPVAVLVAGVALLVVSIGYGVGVFGALSNGGFDDPATESAK
                     ELAAEQAAFDNHDADVVAIYSSDHLTAADPRFEAAVQAVVDDLPGDAVARVQPYYAPQ
                     ARAGAQPMVSDDGHAAMVVLSLTGTTQDEKHDSYEAIKDQLDADGLTTDVGGPWAVFG
                     DVSETVSADIARAESLTMPIVFVLCLVIFGSFVAALMPGMIGAVAVFGAFAALRLLTT
                     VTGVSIFAINIITLIGMGLAIDYALFVVSRFREELRAQPGTDRVHVRHAVVRTMSTAG
                     RTVLFSGLTVAASMAALVLFPQNFLRSMGYGGVAAVLVAMTAALTILPALLAVLGHRI
                     DAGRMPWRRGRRTTLVTGSVASAGWARIAHSVMRRPVAYLVVITAALLALGLPFLSAT
                     WGSVDYRVLPESAPSHQAALVQADRFGGETSTASIMVSGATPDELTAYVDRLADVPGI
                     ATAQVVDRTAAPDGQALVQATWSGNSQTERSHQIVQDLRDVDPGDGASALVGGQSAQS
                     VDLISSIGDHLPWMGLVVVAVMLVLLFLAFGSVVLPLKAVVMNAVSIGASFGVVTWIF
                     AEGHLSGLLGFTSPGFLDATQPILMLGILFGLSMDYEVFLLSRIREEWDRTHDNTTAV
                     ATGLQRSGRIITSAALLLAVVIGGFATSGIVFLKMIGIGMLVAVLLDATVVRALLVPA
                     TMRLLGSANWWAPAPLRRWWERHGMRESDVSDELEPELVGAGQV"
     gene            2956..3738
                     /locus_tag="VH419_16265"
     CDS             2956..3738
                     /locus_tag="VH419_16265"
                     /inference="COORDINATES: protein motif:HMM:NF024701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR-like C-terminal domain-containing protein"
                     /protein_id="HEY7045225.1"
                     /translation="MTATGTEAPLTRRERQRQATYDEIVDVSRRLIAAGDGLSLRAVA
                     AEMGMTAPALYRYVDSYQELVLLVARSIYADVLATLVSARNTYPDDDPGAQVVASAVA
                     FRTWALRHRAEFALLFANPAVSREEGSHPDEDSAAFANFFGDIYRRLWNRYGFAIPTD
                     DELHPEALRSLLALREAGSLPCDFPGMPIGMSWIFIRAWNRLYGTVTLEVFKHMDSGV
                     VSSGALFRAMLEDNGRDLDFGADWSRLLAVADTEMAADAEVT"
     gene            3735..4223
                     /locus_tag="VH419_16270"
     CDS             3735..4223
                     /locus_tag="VH419_16270"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017935998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY7045226.1"
                     /translation="MSGADVVVRPARADEADLLTGLALRSKAHWGYHEDFMAACRDEL
                     TVPATACESGHVLVGELVGEVYGEVAGGVLGFSRIEPAGDVVELVALFVEPSAIGSGL
                     GRLLMDAAVDQAKALGAARIELDADPYAEPFYARMGCVPIGESPSHSIPGRSLPRMAL
                     DF"
     gene            complement(4666..5505)
                     /locus_tag="VH419_16275"
     CDS             complement(4666..5505)
                     /locus_tag="VH419_16275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020576489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY7045227.1"
                     /translation="MSTTSTQQRPEFQRGSLGPRLTLPFLGARPWRVVERNFLVYRHG
                     WFVFATGMLEPILYLLSIGIGVGGLVGDFTLADGSEVGYVEFVAPAMLAASAMNGALF
                     DATYNIFFRMKYEKLYDAMLATPLRPTDVARGEIVWALLRGAIYSAVFIVVMLCLGLV
                     SSWWMVLALPATMLIGYAFAGAGMALTTWMRSWQDFEYIQLSIVPMFLFSATFYPLST
                     YPDTVEAIVRWTPLYQGVVLCRSMSLGTMGWDDLIAVVYLAVMGTLGLVVAARRIATL
                     LLK"
     gene            complement(5502..6359)
                     /locus_tag="VH419_16280"
     CDS             complement(5502..6359)
                     /locus_tag="VH419_16280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012923190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY7045228.1"
                     /translation="MSDKAVLDAGTPADPRRRIHPRLRDFTPTMVPRLRDYWVTVYRR
                     TWRGSVVTSFLMPFLYLTAMGVGLGSFVDANSGPSALDGVSYLAFIAPGLLATTAMET
                     AIGESTYPVMGGFKWHKVFFSMAATPLRIADVVAGQVVFTAFRIVLTCSVFLGVLAAY
                     GALHSAVGGLAALAVVVLLGLAYTTPMVAITSRMKSESGFALVFRLGLMPMILFSGAF
                     FPISQLPQAIEWLAFLTPIWHGVELARSLTLGTVTFWPAVGHLAYLLGWFAVGWYYCI
                     TGFARRLAT"
     gene            complement(6359..7291)
                     /locus_tag="VH419_16285"
     CDS             complement(6359..7291)
                     /locus_tag="VH419_16285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014847715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY7045229.1"
                     /translation="MITARGLRKSYGRTPETRVEAVKGVDVDVHKGEAFGFLGPNGAG
                     KSSTMRMIAAVSPVTEGTLRILGMDPATEGSAIRARLGVCPQEDTLDNELTVLENLVV
                     YGRYFGLSRAEATDRARELLSFVQLTEKSKAKVEDLSGGMKRRLTIARSLINNPDLLL
                     LDEPTTGLDPQARHQLWDRLFRLKQQGVTLAITTHYMDEAEQLCDRLVVMDKGVIVAH
                     GSPAELIVEHSTREVLELRFAVGTDNESMAAKVDDLADRVEALPDRLLLYTPDGEASA
                     AAVYDRGLRPVSSLVRRATLEDVFLRLTGRSLVD"
     gene            7545..8279
                     /locus_tag="VH419_16290"
     CDS             7545..8279
                     /locus_tag="VH419_16290"
                     /inference="COORDINATES: protein motif:HMM:TIGR02983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigE family RNA polymerase sigma factor"
                     /protein_id="HEY7045230.1"
                     /translation="MAGFDEPTSDGVRRHRPALGQPHRTATRPRLEMTSSTTLAFGAG
                     AALGPPLPSPAPGSTGPEPLATLTVPRQRQRSGDDTVREQEREAFDAFVRARLPELLR
                     FGQALTGSPEAAADLVQDALERTLLAWSRLESRDAPEGYVRRIMVNRNISIWRKFGRE
                     RAKDPIEFVDERGAEDHHVDGDLWRALQQLPPKQRAVVALRYYEDMSEADIARTLGCS
                     VGTVKSQASKALAKLRVLVPQDAGEV"
     gene            8279..9535
                     /locus_tag="VH419_16295"
     CDS             8279..9535
                     /locus_tag="VH419_16295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7045231.1"
                     /translation="MSPQRDPDDLGGLEERLRRTFHGERPDVDVESFLGNVHRGARRH
                     RRRQVIGGAVATVAVIGLGAYGVTAAGLFDDNGTPAADQTTAPVRTTSSAPGTATSTE
                     TPPPGQRALSLSATDSERQYVLVAGRFGCRGDCLAAYITDDAGRSWSATSPLGLRPAD
                     PDPTDTTAYAIRFADSSNGWVFGGGLRATHDGGRTWTTPKLPARGIVTTLEAWGDRVY
                     AGVEDYTTETTTLVGSPVDQDAWVTIDVGQKLNNVTEMAVSKDLVAVLASPTPLSEGN
                     EILISTDGQQWTTSPDPCSSAQWPNSVSTSADSLWTVCSSSVGSAVYVSTDAGDSWTV
                     VPGEFFPGSLVQARDDSTAVVVDSNEPGVSIVTVKNQPQHILDDRGDLLPIGFTNPTT
                     GYLRTGDGGILRTLDSGATWQDYPLP"
     gene            complement(9586..10179)
                     /locus_tag="VH419_16300"
     CDS             complement(9586..10179)
                     /locus_tag="VH419_16300"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEY7045232.1"
                     /translation="MDAPWTIDQALDYASGLLRGERVSLRESTEDDFAVLARWWAEPA
                     VSVLQTNFVRPTPATTVSDMMRGWNTNVGNDAGFTIVLNPEGSEGGQPGTVIGQVGLF
                     GIGKNRTATLGIIVGTEYWGQGLGTEALRLAVGYGFVEMGLHRIQLGVYGYNDRAIRA
                     YSKVGFREEGRQREAVYHDRRWHDELLMGILEHEWHS"
     gene            complement(10466..>10702)
                     /locus_tag="VH419_16305"
     CDS             complement(10466..>10702)
                     /locus_tag="VH419_16305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7045233.1"
                     /translation="PTPNDRVSRPGSALSRVGISVLRPKIIGSPLLDVARVRTPLRGT
                     VFRVGLPSLATRKWLIRQVMARWEVCHVYSPKGV"
BASE COUNT         1775 a         3699 c         3641 g         1587 t
ORIGIN      
        1 cggtcatgtt gaccagcgac cagccttggg cggcgtacca ctgtgactcg gcccgggtgg
       61 agaaccgcgg cccttggacg acgaccatcg ctcccccgtc cacgatcgtg tcgtccacgc
      121 ccagcagcgc tgtacgcagg ctcgggcagt acgggtcggc gaactccacg tggcacgcgc
      181 cgacgtcgta gaacgtctgg acccggttgg tggtccggtc gaccagctgg tcgggaacga
      241 cgagcgtgcc gggcccgagc tcggcctgca ggctgccgac cgcgcacggc gcgaggacgc
      301 ggcgtacgcc gagcgagcgc agcgcccaca ggttcgctcg gtagttcacc tggtgggcgg
      361 ggaactcgtg tcgtctcccg tgccgcggga ggaaggccac cggccttccg tgcacggccc
      421 cgacggagac cggcgctgac ggctcgccgt acggcgtggc catggtcacg tcgtcgggcg
      481 cgtcgaggaa ccggtagaag cccgaaccgc cgatgacggc gatctcggca cgagcgtcgg
      541 gcatgcgcgc cagtctggca gccaggtgcg cggaagcgcc cttcgacccg gacgggacga
      601 agggcgctcc gtcgacggga cgcctctcgg tcagacctga ccagcgccca ccagctcggg
      661 ctcgagctcg tcgctgacat cggactcacg catgccgtgc cgctcccacc aacggcgcag
      721 cggagctggc gcccaccagt tcgccgagcc gagcagccgc atggtcgccg gaacgaggag
      781 cgcccggacg accgtggcgt cgagcaggac ggccacgagc atgccgatcc cgatcatctt
      841 gaggaagacg atccccgacg tggcgaaccc cccgatcacg acagcgagca ggagtgcggc
      901 gctggtgatg atgcgaccac tgcgctgcag gccggtggcg accgctgtgg tgttgtcgtg
      961 ggtcctgtcc cactcctcgc ggatccgcga gagcaggaac acctcatagt ccatcgagag
     1021 accgaacagg attcccagca tgaggatcgg ctgggtggca tccaggaatc cgggcgacgt
     1081 gaacccgagc agtccggaca ggtgaccttc cgcgaagatc cacgtcacga cgccgaacga
     1141 cgcgccgatc gacacggcgt tcatcaccac cgccttgagc ggcagcacga ccgacccgaa
     1201 ggccaggaag agcagcacga gcatcaccgc gacgaccacc aggcccatcc agggcaggtg
     1261 gtccccgatc gagctgatca agtcgacgct ctgcgcggac tgtccgccca ccagtgccga
     1321 agcgccgtcg ccagggtcga catcgcgcaa gtcctgcacg atctgatgag agcgctccgt
     1381 ctggctgttg ccggaccagg tcgcctgcac gagtgcctgt ccgtcaggtg cggcggtccg
     1441 gtcgaccacc tgtgcggtcg cgatgccggg gacgtcggcc agccggtcga cgtacgccgt
     1501 cagctcgtcg ggcgtcgcgc cggacaccat gatgctggcc gtcgacgtct cgccgccgaa
     1561 ccggtcggcc tggacgaggg ctgcctgatg gctcggtgcc gactcgggga gtacgcggta
     1621 gtccacgctt ccccacgtgg ccgacaggaa cggcaggccg agcgccagca gcgccgccgt
     1681 gatgacgacg aggtaggcga ccggacgcct catcacgctg tgcgcgatcc tcgcccagcc
     1741 cgcggaagcc accgatccgg tgacgagggt ggtccggcgg ccgcggcgcc acggcatccg
     1801 gcccgcgtcg atccggtgac cgagcacggc gagcagggcg ggcaggatcg tcaacgcggc
     1861 ggtcatcgcc accagcaccg ccgcgacgcc gccgtacccc atggagcgca ggaagttctg
     1921 cgggaagagc accagcgccg ccatggaggc cgccacggtc aagccggaga agagcaccgt
     1981 gcggccggcc gtcgacatgg tgcgcaccac tgcgtggcgg acgtggaccc gatcggtgcc
     2041 cggctgtgcc cggagctcct cgcggaagcg gctgaccacg aacaacgcgt agtcgatcgc
     2101 gaggcccatc ccgatcagcg tgatgatgtt gatcgcgaag atcgagacgc cggtcacggt
     2161 ggtgagcaga cgaagggcgg cgaacgcgcc gaacaccgcc accgctccga tcatcccggg
     2221 catcaatgcg gcgacgaacg agccgaagat gacgaggcag agcacgaaca cgatcggcat
     2281 ggtgagcgac tcggccctgg cgatgtcggc gctgacggtc tcgctcacgt caccgaacac
     2341 cgcccacgga ccgccgacgt ccgtcgtcag accgtcggcg tcgagctggt ccttgatcgc
     2401 ctcgtagctg tcgtgcttct cgtcctgggt cgtgcccgtg aggctcagca cgaccatcgc
     2461 ggcgtggccg tcgtcgctca ccatcggctg tgcgccggcc cgggcctgcg gtgcgtagta
     2521 gggctggacc cgcgccacgg catcgccggg caggtcgtcg accaccgcct gcacagccgc
     2581 ctcgaaccgt gggtccgcgg cggtcaggtg gtcgctggag tagatcgcca cgacgtccgc
     2641 gtcgtggttg tcgaacgctg cttgctcggc cgcgagctcc ttcgccgact cggtggcggg
     2701 atcgtcgaac ccgccgttgc tgagggcgcc gaagaccccg acgccgtacc cgatgctgac
     2761 gacgagcagc gccacccccg ccacgaggac ggcgacgggc cgacgtgcca cgaacgttcc
     2821 ccatcgctga accatgctga cctccgcaat gctcaatcac ttctgctaac atcgtttact
     2881 gtttacgatg ttcactctct tgtcaagcgt ttatgcttgg gccagtgaag ctgcgacaga
     2941 agaagggagt ccgcgatgac ggccaccggc accgaggctc cgctgacccg tcgcgagcgg
     3001 cagcggcagg cgacgtacga cgagatcgtc gacgtgtcgc gacggctcat cgcggccggt
     3061 gacggcctgt cgttgcgcgc ggtggcggcc gagatgggga tgaccgcgcc ggcgctgtac
     3121 cggtacgtcg acagctacca ggaactcgtg ctgttggtgg cccgctcgat ctacgccgac
     3181 gtactcgcga cgctggtctc ggcgcgcaac acgtaccccg acgacgatcc cggcgctcag
     3241 gtcgtcgcgt ccgcggtcgc cttccgcacg tgggcgttgc gccatcgagc ggagttcgcg
     3301 ctgctgttcg ccaaccctgc ggtctcccga gaggaggggt ctcaccctga cgaagactca
     3361 gccgcgttcg ccaacttctt cggcgacatc taccgccggc tgtggaaccg ctacggcttc
     3421 gcgatcccaa ccgacgacga gctccacccg gaggcgctgc gctcgctgct cgcgctacgc
     3481 gaggccggat cgctgccgtg cgacttcccc ggcatgccga tcggcatgag ctggatcttc
     3541 atccgggcgt ggaaccggct gtacggcacc gtgacgctcg aggtgttcaa gcacatggac
     3601 agcggtgtcg tctcgtccgg cgcgttgttc cgcgcgatgc tcgaggacaa cggccgcgac
     3661 ctggacttcg gtgccgactg gtctcgcctg ctggccgtcg ccgacacgga gatggctgcc
     3721 gacgctgagg tcacgtgagc ggcgccgacg tcgtggtccg gcccgcccgg gccgacgaag
     3781 ccgacctcct caccggcctc gcgctgcgga gcaaagcgca ctggggctac cacgaggact
     3841 tcatggccgc gtgccgcgac gagctgacgg tgcccgcgac cgcctgcgag tccggccacg
     3901 tcctggtcgg cgaactggtc ggcgaggtgt acggcgaggt ggccggcggg gtgctgggct
     3961 tctcgcgcat cgaaccggct ggtgacgtgg tcgagctggt cgcgctgttc gttgagcctt
     4021 ctgcgatcgg gtccggactc gggcggcttc tgatggacgc agccgtcgat caggcgaagg
     4081 ccctgggcgc tgctcgcatc gagctggacg ccgacccgta cgccgagccg ttctacgccc
     4141 ggatgggctg cgtccccatc ggcgagtcac ccagccactc gatcccgggc cgctcccttc
     4201 cccgcatggc gctggatttc tgaagtggcc cgctgatcga gctcgtcgag atcgaggggt
     4261 ctcgacaagc tcgaccaccg aagcgtgccg gccacccttg aggcctggga atgccgcgtt
     4321 ccgccccaat aggacaagtg actgtggtgg tttgcgatgt ttacaggcca agtgggctgt
     4381 aaacatcgca aacctccgat gggcagaggg ggtttgggat gtgaacatcg caaccgtggg
     4441 accgctgagc ggtgagcggt gggcggtccg gaaaccgaca gcacacacac cgagcaaatc
     4501 ggcggggcgc cggctgaccg gctcaaccac gcagcaaggt ctcccgctcg tctttgccga
     4561 tgcacctcag cccacgacga cagagcgttg ggcgaacggg cgcaccaacc gcggcctgca
     4621 ccgcggacgg cataccgaag gaatcacctg ggcaaccggc aacagtcact tgaggagcag
     4681 ggtggcgatg cgacgggcgg cgaccacgag gccgagggtg cccatcaccg ccaggtagac
     4741 cacggcaatc aggtcgtccc agcccatcgt gccgagcgac atgctgcgac acagcaccac
     4801 accttggtac agcggggtcc agcgcacgat ggcctcgacc gtgtcggggt acgtcgacag
     4861 cgggtagaac gtggccgaga acaagaacat cgggacgatc gacagctgga tgtactcgaa
     4921 gtcctgccag gaccgcatcc acgtcgtcag cgccatcccg gctccagcga acgcgtagcc
     4981 gatcagcatg gtcgccggca gcgccagcac catccaccac gacgagacca gcccgaggca
     5041 cagcatcacc acgatgaaga ccgccgagta gatcgcgccg cgcagcaacg cccagacgat
     5101 ctcgccgcgc gcgacgtcgg tcggccgaag cggcgtggcc agcatcgcgt cgtagagctt
     5161 ctcgtacttc atccggaaga agatgttgta cgtcgcgtcg aacagcgcgc cgttcatggc
     5221 ggaagcggcc agcatcgccg gcgcgacgaa ctcgacgtac ccgacctcgc tcccgtccgc
     5281 caaggtgaag tcgccgacga gcccgccgac gccgatgccg atcgacagca ggtagaggat
     5341 cggttcgagc atcccggtcg cgaagacgaa ccagccgtgc cggtagacga ggaagttgcg
     5401 ctcgacgacc cgccagggac gcgccccgag gaagggcagg gtcagccgcg gtccgaggct
     5461 cccccgctgg aattccggtc gttgctgcgt cgaggtcgtg ctcatgtcgc caacctgcgc
     5521 gcgaagccgg tgatgcagta gtaccagccg accgcgaacc agccgagtag gtacgccaag
     5581 tgtccgaccg ccggccagaa cgtcaccgtc ccgagcgtga ggctgcgggc cagctcgacg
     5641 ccgtgccaga tcggcgtgag gaacgccagc cactcgatcg cctgtggcag ctggctgatc
     5701 gggaagaacg cgccgctgaa caggatcatg ggcatgaggc cgagtcggaa gacgagcgcg
     5761 aagccgctct cgctcttcat ccgcgaggtg atcgccacca tgggcgtcgt gtaggccagg
     5821 ccgagcagca cgacgacggc gagcgccgcg agtccgccca ctgcactgtg cagggcgccg
     5881 tacgccgcca gcaccccgag gaacaccgag caggtcaaca cgatgcggaa ggcggtgaac
     5941 acgacctgcc cggccacgac gtcggcgatg cgcagcggag tcgccgccat cgagaagaac
     6001 accttgtgcc acttgaagcc gcccatcacc gggtaggtcg actccccgat cgcggtctcc
     6061 atcgcggtcg tggccaacag gccaggggcg atgaaggcga gatagctgac cccgtccaga
     6121 gcgctcgggc ccgagttggc gtcgacgaag ctgccgagcc ccacccccat cgccgtcagg
     6181 tagaggaacg gcatcaggaa gctggtgacc accgagccac gccaggtacg ccggtagacg
     6241 gtcacccagt agtcgcgtag tcgcggcacc atcgtcgggg tgaagtcgcg gagtcgaggg
     6301 tggatacgcc ggcggggatc ggccggcgta ccggcgtcga ggacggcctt gtcgctcatc
     6361 agtccaccag cgaccggccg gtcaaccgga ggaacacgtc ctccagcgtc gccctccgga
     6421 ccaaggacga gaccgggcgg agtccccggt cgtagacggc cgccgctgat gcttcgccgt
     6481 ccggggtgta gagcagcagc cggtcgggca gcgcctcgac gcggtcggcg aggtcgtcga
     6541 ccttcgcggc catcgactcg ttgtccgtac cgacagcgaa ccgcagttcg aggacctcgc
     6601 gcgtggagtg ctccacgatg agctcggccg gtgagccgtg cgcgacgatc acgcccttgt
     6661 ccatcaccac gagccggtcg cacagctgct cggcctcgtc catgtagtgg gtcgtgatgg
     6721 cgagcgtcac gccttgctgc ttcagccgga acagccggtc ccacagctgg tgccgggcct
     6781 gagggtcgag cccggtcgtc ggctcgtcga gcagcagcag gtcagggttg ttgatgagcg
     6841 agcgggcgat cgtcagcctg cgcttcatgc caccggacag gtcttcgacc ttcgccttgg
     6901 acttctcggt cagctgcacg aagctgagca gctcgcgagc ccggtcggtg gcctcggccc
     6961 ggctgaggcc gaagtagcgg ccgtagacga cgaggttctc cagcacggtc agctcgttgt
     7021 cgagcgtgtc ctcctgcgga cagacgccga gccgagccct gattgccgac ccttccgtcg
     7081 cggggtccat gccgaggatc cgcagcgtcc cctcggtcac cggcgagacc gcggcgatca
     7141 tccgcatcgt ggaggacttc ccggcgccgt tcggtccgag aaacccgaag gcctccccct
     7201 tgtgcacgtc gacgtcgacg cccttgaccg cctcgaccct ggtctcgggc gtccgaccgt
     7261 acgacttgcg caggccacga gcggtgatca tcagctgcgc ctcgctgcgc gactgctgag
     7321 ccgccgactg gggactgcgg gtgggcaggt cggacgtcac gaggtacgac cctaacttcc
     7381 ggcaccgacg cttcccaacc ggataacttc tgaccaccgt ggccacaacc gcgccggtga
     7441 cacagtcgtg acggacccat gaccgagccg tgacggggcc atcacgggga tggtcacggg
     7501 tctgtccgca ccggatggca agatgagcgc tgcgacccgg gagcttggcc ggtttcgacg
     7561 agccgacctc ggacggcgta cgccgacatc gtcccgcgct cgggcaacca catcgcaccg
     7621 cgacgcgtcc tcggctcgag atgacaagtt ccacgacgct tgcgttcggc gcgggggcgg
     7681 cgctcggacc cccgctgccg tcgccggcac ccggatccac cgggccggag ccgttggcga
     7741 cgctcacggt gccacggcag cggcagcgat cgggggacga caccgtgcgc gagcaggaac
     7801 gcgaggcgtt cgacgcgttc gtgcgcgccc ggcttcctga gctcctgcgc ttcggccagg
     7861 ccctgacggg gtcacccgaa gccgccgccg atctggtgca ggacgcgctc gagcgcacct
     7921 tgctggcctg gtcgaggctg gagtcacgcg atgcgccgga aggctacgtg cggcggatca
     7981 tggtcaaccg caacatcagc atctggcgca agttcgggcg ggagcgcgcc aaggacccca
     8041 tcgagttcgt cgacgagcgc ggcgcggagg accaccacgt cgacggtgac ctgtggcgcg
     8101 ccctgcagca gctgccgccg aagcagcgtg ccgtcgtcgc cctgcgctac tacgaggaca
     8161 tgtcggaggc cgacatcgcg cgcaccctcg ggtgctcggt cggcaccgtg aagagccagg
     8221 cgtcgaaggc gcttgccaag ctgcgggtgc tcgtgccgca agacgcgggg gaggtgtgat
     8281 gagtccgcag cgcgaccctg acgacttggg cggcctcgag gagcgcctgc gccgtacctt
     8341 ccacggggaa cgccccgacg tggacgtcga gtcgttcttg ggcaacgtgc accgcggcgc
     8401 ccgacggcac cgtcgccgcc aggtgatcgg tggcgccgtg gccaccgtgg ccgtgatcgg
     8461 tctcggcgcg tacggcgtca cggccgccgg actcttcgac gacaacggca cgcccgcggc
     8521 cgaccagacc acggccccgg tcaggacgac gtccagcgca ccgggcaccg cgacgtccac
     8581 cgagacgcct ccgccgggcc agcgagcgtt gtccctgtcg gcgacggaca gtgagcgtca
     8641 gtacgtgctc gtcgcgggga ggttcggctg tcgcggtgac tgcctggcgg cctacatcac
     8701 cgacgacgcg ggccggtcct ggtcagcgac gtctccactc gggctccgac ctgctgaccc
     8761 ggaccccacc gacacgacgg cgtacgcgat ccgattcgcc gacagcagca acggttgggt
     8821 cttcggtggc ggcctgcggg cgacccacga cggcggccgc acctggacga cgccgaagct
     8881 gccggcgcgc ggcatcgtca cgacgctgga ggcctggggc gaccgcgtgt atgccggggt
     8941 cgaggactac acgaccgaga ccaccacgct ggtcggctca cccgtggacc aggacgcgtg
     9001 ggtcaccatc gacgtcggtc agaaactgaa caacgtcacg gagatggcgg tgtcgaagga
     9061 cctcgtcgcc gtactggcca gccccacacc actgagcgaa ggcaacgaga tcctgatcag
     9121 caccgacggt cagcagtgga cgacgagccc tgatccgtgt tcatcggccc aatggccgaa
     9181 cagcgtctcg accagcgccg actcgttgtg gacggtgtgc tcgagctctg tcggatcggc
     9241 cgtctacgtc agcaccgacg ccggtgactc gtggaccgtc gtacccggtg agttcttccc
     9301 ggggagcctc gtccaagcca gggacgattc caccgctgtc gtcgtggaca gcaacgagcc
     9361 cggggtctcg atcgtgaccg tcaagaacca gccgcagcac atcctcgacg accgcggtga
     9421 cctgttgccg atcggtttca cgaacccgac cacgggctac cttcgcacgg gcgacggcgg
     9481 catcctgcgc acgctcgaca gcggcgccac ctggcaggac taccccttgc cctagctttc
     9541 gtcaccctgt gctccccgcg acgtgcgggt cggcgtggtc gctggtcagg agtgccactc
     9601 gtgctcgagg atgcccatca ggagctcgtc gtgccatcgc cggtcgtggt acaccgcttc
     9661 ccgctggcgc ccctcctccc ggaacccgac cttgctgtag gcgcggatcg cgcggtcgtt
     9721 gtagccgtac acgccgagct ggatccggtg caggcccatt tccacgaagc cgtacccgac
     9781 ggccaaccgg agcgcctccg tcccgagccc ctggccccag tactccgtcc cgacgatgat
     9841 gccgagggtc gcggtccggt tcttcccgat cccgaaaagg ccgacctggc cgatgacggt
     9901 gcccggctga ccgccttccg aaccttcggg gttcagcacg atggtgaacc cggcgtcgtt
     9961 gcccacgttg gtgttccagc cccgcatcat gtcgctgacc gtggtcgccg gggtgggccg
    10021 gacgaagttc gtctggagca ccgagaccgc aggctcggcc caccaacggg ccaggacagc
    10081 gaaatcgtcc tcggtcgact cgcgcaggct gacccgctct cctcgcagca gcccgcttgc
    10141 gtagtcgagt gcctgatcga tcgtccaggg tgcgtccatc cgccgatgct cgcagtggcg
    10201 ggcataccgg gcaagtcagt tcgaccctgc tcggacacgg tcgtggtctg tgggcgtttt
    10261 cagtcggcgt accggtcggc gtcggctgag ggtgagtcgc aaagacgagc gggagacgtt
    10321 cgtgcccgct gggctggtcg gccggcgctc cgtccctcct tacctggcgt ctgactggcg
    10381 gttttcggac atgcgcggtt gaccggcccg ctgtttgcga tgttcagacg gcaaaccccc
    10441 tctgcccatc ggaggtttgc gatgtttaca cgccttttgg gctgtaaaca tggcaaacct
    10501 cccaacgcgc catgacctgg cggatcagcc attttcgggt ggccagcgag ggcagaccga
    10561 cccggaatac ggtgccccga aggggtgttc gcactcgtgc cacgtcgagg agaggactgc
    10621 cgatgatctt cgggcgcaag accgagatgc ccacccgcga caacgcgctt cccggccgcg
    10681 acacgcggtc gttcggcgtc gg
//
LOCUS       DATBFZ010000532         6343 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_3868767, whole genome shotgun sequence.
ACCESSION   DATBFZ010000532 DATBFZ010000000
VERSION     DATBFZ010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 6343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6343
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3868767"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1408
                     /locus_tag="VH419_16310"
     CDS             <1..1408
                     /locus_tag="VH419_16310"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEY7045234.1"
                     /translation="DAPLRRTIQKAQDGSPYVCLRQDDVNLTSPPLASTCHDAAHGTP
                     DSAFENEPFTIDDYIAPTDTTCPPPGVFAPNGIPNGQGEPGGCTRDLVHRFYQEKYQI
                     DHGKQDHYVTGSDAIGLTMGIYDTTQLPIYQYLHSSGAPHYVIADHFFQGANGGSFFN
                     HQWLIAGRAPVVDQSAPVPDPTKRPVNSVLDDNGMPNTYPQYTPTGPVVDGQLTVTCR
                     NPANVDNYRKACGNFAVNTVQPSSPPFSAGGAFIPLIDDSQYPNIGDRLTAKGISWAW
                     YSGGWDDAAAGHPGPLFQYHHQPFNYFAAYAPGTPGRHHLQDETDFFDAAAIGNLPTV
                     SFVKPYGAENEHPGYASANSGSMHLVDLVKAVVEGPQADDTLVVITYDEFGGQWDHVS
                     PPGPGSSTRGPYDAFGPGTRIPALLLSARMHRSGVDHRSYDTTSIIATLERSFHLAPV
                     GIRDTRVADLRHAIRLGN"
     gene            complement(1465..2490)
                     /locus_tag="VH419_16315"
     CDS             complement(1465..2490)
                     /locus_tag="VH419_16315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004558616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEY7045235.1"
                     /translation="MRVGVLTGGGDCPGLNAVIRAVVRKGVGEYGYDFVGFRDGWRGP
                     LENITSDLGIPQVRGILPRGGTILGSSRTNPFSVENGVERITDNLRANRVDALVAIGG
                     EDTLGVATRLSDLGVNVVGVPKTIDNDLSGTDFTFGFDTAVNIATEAIDRLHTTAESH
                     HRVLVVEVMGRHTGWIALHSGLAGGANVILIPERPFEIDKVCKLVEQRFQLQYAPIIV
                     VAEGAVPAEGTMQTVSEEPDEFGHVRLGGIGDRLAAEVQTRTGKESRAVVLGHIQRGG
                     TPTAFDRWLATRFGLHAIQAVHDGEYGTMVALRGTQIAHVDLTEGTGELKTVDPELYA
                     EAEVLFG"
     gene            2645..3133
                     /locus_tag="VH419_16320"
     CDS             2645..3133
                     /locus_tag="VH419_16320"
                     /inference="COORDINATES: protein motif:HMM:NF012982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008138 - protein
                     tyrosine/serine/threonine phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HEY7045236.1"
                     /translation="MSAPFGADGKGLPNFSYVVDDKLAGMAHPRYAGDLGEALDALKA
                     EGVTALVSLDEVGLDSDLVRRHGLVHRHIPVPDFGAPTAKQADAFVAFVDAEIERGGA
                     VVAHCGAGIGRTGTMLAAYLIAHGSSVGAATEEVSRRRTQSHESAQQLEFLREYAGRR
                     TG"
     gene            complement(3137..3979)
                     /locus_tag="VH419_16325"
     CDS             complement(3137..3979)
                     /locus_tag="VH419_16325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007078095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY7045237.1"
                     /translation="MFYWLLKWVLIGPLLKFIFRPWAEGTENVPEEGGAILASNHLSY
                     SDWLFMPVTIPRRVTFVAKAEYFTTPGFKGWMQKTFFSGAGQVPIDRSSGTAAAGAIA
                     TGLRILASGELFGIYPEGTRSADGRLYRGKTGVARMALEAKVPVIPIAVVDTDIVAPP
                     GKVFGRFGRPGVRFGKPLDFSRYEGLEDDRFVLRSITDEIMYEIMQLSGQEYVDVDST
                     TYKAALAKESKEAKAEARAEAKEAKAEAKLEAKLEAQAAAEAVARAEQADDDDEPGPA
                     SASG"
     gene            4158..4934
                     /locus_tag="VH419_16330"
     CDS             4158..4934
                     /locus_tag="VH419_16330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7045238.1"
                     /translation="MRSNGITAPAYTPVADLDPRVADALLGELKQRGVAAYTKPVESS
                     SIPGLDRPEFRVSALDRLYVDARESEQVKALITAHDPGLVTSSDDLTWAQIVAGFDQP
                     MATQEHPWPVREDVGRSGGDDHGSVNADADGGTELRQWLSRRDATWTEDEPGDGFGET
                     VARGTRRHDQEAGDDAGDRFVPDPPPPLPHLEPLQQLAWVGLIGGPLLLLAAVLFSIS
                     LPTWVALLAVAGFVGGFVTLVATMEDKSGSEDDPENGAVV"
     gene            4945..5298
                     /locus_tag="VH419_16335"
     CDS             4945..5298
                     /locus_tag="VH419_16335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7045239.1"
                     /translation="MIPVLEAVVVVLAVVVTLLALVAVIRDREPDWWLAGALVALELA
                     LIVQCVVGLVRLGSTDRDVDGLTFAAYLVLALLVPPAGFLWAATERTRWGTAVIAVAG
                     VVVVVMVFRLDQVWG"
     gene            5306..5776
                     /locus_tag="VH419_16340"
     CDS             5306..5776
                     /locus_tag="VH419_16340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020579314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7045240.1"
                     /translation="MTVKRAQRSEDGPDGGRDPQSAPPATRSGFGRVLIAVYAVFAIA
                     ATGRSAYQLSVKASDAPIPYTLSLVAALIYVTATVALFRGGPTWRRVAWVACSIELIG
                     VLVVGTVSVLVEFPDATVWSDYGIGYGFVPLVLPIFGLWWLRKTAPPQEGGLRP"
     gene            5895..>6343
                     /locus_tag="VH419_16345"
     CDS             5895..>6343
                     /locus_tag="VH419_16345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00300 family protein"
                     /protein_id="HEY7045241.1"
                     /translation="MSTHDTETVEITGHLMDSGILSMVLSDIRDYGGDHVVEKFDLGH
                     EATDMSYARIVVSAQDEESLQRMLMRLQTRGVNMVDPGEVTVVAAEQDGVFPDGFYST
                     TNLQTRVRYQGKWVQVSNPEMDCGLIIDDSADETRVSTLPMSDVKAG"
BASE COUNT         1014 a         2218 c         2105 g         1006 t
ORIGIN      
        1 cgacgcgcca ctgcgtagga cgatccagaa ggcgcaggac ggttcgccgt acgtctgcct
       61 gcgtcaagac gacgtcaacc tgacgtcgcc tccgctggcg tcgacctgcc acgacgcggc
      121 acacggtacg ccggacagcg ccttcgagaa cgagccgttc accatcgacg actacatcgc
      181 gccgaccgac acgacctgcc cgccgcctgg ggtcttcgcg cccaacggca taccgaacgg
      241 tcaaggtgag ccgggtggct gcacacgtga cctggtgcac cgcttctacc aggagaagta
      301 ccagatcgac cacggcaagc aggaccacta cgtcaccgga tccgacgcca tcggactcac
      361 gatggggatc tacgacacga cccagctgcc gatctaccag tacctgcact cctccggcgc
      421 accgcactat gtgatcgccg accacttctt ccagggcgcc aacggcggct cgttcttcaa
      481 ccaccaatgg ctcatcgccg gccgtgcgcc cgtcgtcgac cagagcgcac cggtgcctga
      541 tccgaccaag cggccagtca actccgtgct ggacgacaac gggatgccga acacctaccc
      601 gcagtacacc ccgaccggcc cggtcgtcga cggccagctc accgtgacgt gtcggaaccc
      661 ggcgaacgtc gacaactacc ggaaggcctg cggcaacttc gcggtcaaca ccgtgcagcc
      721 ctcgagccca ccgttcagcg cgggtggcgc gttcatcccg ctcatcgacg actcgcagta
      781 cccgaacatc ggcgaccggc tcacggccaa gggcatctcg tgggcgtggt actccggcgg
      841 ttgggatgac gcggcagccg gtcaccccgg cccgctcttc cagtaccacc accagccgtt
      901 caactacttc gcggcgtacg cacccggcac gcccggccgg caccatctgc aggacgagac
      961 ggacttcttc gacgcggcgg cgatcgggaa cctgccgacg gtcagcttcg tcaagccgta
     1021 cggcgccgag aacgagcacc ccggctacgc gagcgccaac agcggcagca tgcacctcgt
     1081 cgacctcgtg aaggccgtcg tggaaggtcc gcaggccgac gacacgttgg tggtcatcac
     1141 ctatgacgag ttcggcggac agtgggacca cgtgtcgcca cccgggcccg gcagctcgac
     1201 acgaggcccg tacgacgcct tcggtccggg cacccggatc ccggcactgc tgctgtcggc
     1261 gcggatgcac cgctccggcg tggaccatcg gtcgtacgac acgacctcga tcatcgccac
     1321 tctggagcgc tccttccacc tcgcaccggt cgggatccgc gacacccggg tggccgacct
     1381 ccgccacgcc atccgcctgg gtaactgagt tgtcagaagc ccatggagct ataggcccat
     1441 gagcttctga caagtcggcg ggcgtcagcc gaagagaacc tcggcctcgg cgtacagctc
     1501 ggggtcgacg gtcttcagct cgcccgtccc ctcggtgagg tcgacgtggg cgatctgagt
     1561 cccgcgcagt gcgaccatcg tgccgtactc gccgtcgtgg accgcctgga tcgcgtgcag
     1621 accgaaccgg gtggcgagcc agcggtcgaa cgccgtcggc gtaccgccgc gttggatgtg
     1681 tccgagcacg accgcgcggg actccttgcc ggtacgcgtc tgcacctcgg cggcgagccg
     1741 gtccccgatc ccgccgagcc gcacgtgccc gaactcgtcg ggctcctcac tcaccgtctg
     1801 catggtgccc tcggccggca cggcgccttc ggcgacgacg atgatcggtg cgtactggag
     1861 ctggaaccgc tgctcgacca gcttgcacac cttgtcgatc tcgaacggcc gctcggggat
     1921 caagatgacg ttcgcaccac cggcgagtcc ggagtgcagc gcgatccagc cggtgtgccg
     1981 ccccatcacc tcgaccacga gcactcgatg gtgcgactcg gcggtcgtgt gaaggcggtc
     2041 gatcgcctcg gtggcgatgt tgaccgcggt gtcgaagccg aacgtgaagt ccgtcccgga
     2101 caggtcgttg tcgatcgtct tgggtacgcc gacgacgttc accccgaggt cggacagcct
     2161 cgtcgccacc cccagcgtgt cctcaccgcc gatcgcgacc agtgcatcga cccggttggc
     2221 ccgcaggttg tcggtgatgc gctccacgcc gttctcgaca ctgaacgggt tcgtgcgtga
     2281 cgagccgagg atggtgccgc cgcgcggcag gatgccccgg acctgcggga tgccgagatc
     2341 gctcgtgatg ttctcgagcg gaccgcgcca gccgtcgcgg aacccgacga agtcgtagcc
     2401 gtactcgccg acgcccttgc gcaccacggc gcggatcacc gcgttgaggc ccggacagtc
     2461 tccaccaccc gtcagcacgc cgacccgcat gtgcactcct ccatgccgct gctcagctgt
     2521 gtttcgtccc ggccccaacc tagccagcgc aggcgaacga tggtcagagc ctgggacctg
     2581 tggtccggat gatggacagg gatgctcccc ctcgggccag ggcctgtctg gcacagtgac
     2641 ggccatgagc gcaccgttcg gcgcggacgg gaaaggcctc ccgaacttca gctacgtcgt
     2701 cgacgacaag ctcgcgggga tggcacatcc gcggtacgcg ggcgatctcg gcgaagccct
     2761 cgacgccctg aaggccgaag gcgtcaccgc tctcgtctct ctcgacgagg tcggtctcga
     2821 cagcgacctg gtacgacggc acggcctcgt gcaccggcac atccccgtcc cagacttcgg
     2881 ggcgccgacc gcgaagcagg ccgacgcgtt cgtggcgttc gtggacgccg agatcgaacg
     2941 tggcggcgca gtggtggcgc actgcggtgc gggcatcggc aggacgggga cgatgctggc
     3001 ggcgtacctc atcgcccacg ggtcgtcggt cggggccgcc accgaggagg tctcgcggcg
     3061 acgcacgcag tcccacgagt cagcgcaaca gctggagttc cttcgcgagt acgccggccg
     3121 ccgaaccggg tgaggctcag ccgctggcgg acgccggtcc cggctcgtcg tcgtcgtcgg
     3181 cctgctcagc acgcgccact gcctcggcgg ccgcctgcgc ctcaagcttg gcctcgagct
     3241 tcgcctcggc tttggcctcc ttcgcctccg cccgggcttc cgccttcgcc tccttcgact
     3301 ccttcgccaa cgctgccttg tacgtcgtgg agtccacgtc gacgtactcc tgaccggaca
     3361 gctgcatgat ctcgtacatg atctcgtcag tgatcgagcg cagcacgaag cggtcgtcct
     3421 cgaggccttc gtatcgggag aagtcgagcg gcttcccgaa ccgcacgccc ggccgcccga
     3481 acctgccgaa gaccttgcca ggcggcgcga cgatgtcggt gtccaccacg gcgatcggga
     3541 tcaccgggac cttcgcctcc agcgccatcc gggcaacgcc cgtcttaccg cgatacagac
     3601 ggccatcggc ggagcgggtg ccctcggggt agatgccgaa cagctcgccg ctggcgagga
     3661 tacgcaaccc ggtcgcgatg gcgcctgcgg ccgcggtccc actcgaacgg tcgatcggca
     3721 cctggccggc tccggagaag aacgtcttct gcatccagcc cttgaacccg ggcgtcgtga
     3781 agtactccgc cttcgctacg aacgtcacgc gacgcggaat ggtcactggc atgaacagcc
     3841 agtcggagta ggacaggtgg ttgctcgcga ggatggcgcc gccctcttcg ggaacgttct
     3901 ccgtcccctc cgcccagggt ctgaagatga acttcaacag cgggccgatc aagacccact
     3961 tcagcaacca gtagaacaac gttcgactcc ttgtcgccgc acggtctggg cacacactag
     4021 acgccaccgc caggcaacga aaccggggtt tgcgggaaga cggtcggcca atgggccgtg
     4081 accccagccg ttttcccgca aacagcgggc ggacccggaa gtcgtcagcg gcttggccgg
     4141 catgacacga tgaggatgtg cgttccaacg gcatcaccgc cccggcgtac acgccggtcg
     4201 cggacctcga cccgcgagtc gccgacgcgt tgctcggcga gctcaagcag cggggcgttg
     4261 cggcgtacac gaagccggtc gagtcgtcgt ccatcccggg gctggaccga ccggagttcc
     4321 gggtgagcgc tctggaccgg ctgtacgtcg acgcgcgcga gtcggagcag gtcaaggcgc
     4381 tgatcaccgc ccacgacccc gggctcgtca cgagcagcga cgacctgacc tgggcgcaga
     4441 tcgtggccgg cttcgaccag ccgatggcga cgcaggagca tccctggccg gtgagagagg
     4501 atgttggtcg ctcaggcggc gacgaccatg gttccgtgaa tgccgatgct gacggcggga
     4561 ccgagctgcg gcaatggctc tcccgtcggg acgcgacctg gacggaggac gagcccggcg
     4621 acgggttcgg cgagaccgtg gcccgcggga cgaggcgtca cgaccaggag gccggcgacg
     4681 acgcgggcga ccgcttcgtg cctgatccac cgcctccgct cccgcacctc gaaccgctgc
     4741 agcagctggc ctgggtcggg ctgatcggcg gtccgctgct gctgctcgcg gccgtgctgt
     4801 tctcgatctc gctgccgacc tgggtcgccc tcctggcggt tgccgggttc gtcggaggct
     4861 tcgtcaccct ggtcgcgacc atggaggaca agtcgggttc ggaggacgat ccggagaacg
     4921 gcgcggtcgt ctgacgctgc tgacgtgatt cctgtcctcg aagcggtcgt cgtcgtgctc
     4981 gctgtcgtcg tcacgctgct cgcactggtc gccgtcatcc gcgacaggga accggactgg
     5041 tggctcgccg gtgcgctggt cgcactcgag ctggcgctga tcgtgcagtg cgtcgtcggc
     5101 ctggtccggc tcggcagcac agaccgagac gtcgacggcc tgacgttcgc ggcatacctc
     5161 gttctggcgc tcctcgtccc cccggccggc ttcctgtggg cggccaccga acgcacccga
     5221 tggggaaccg cggtgatcgc ggtcgccggc gtcgtcgtcg tcgtgatggt gttccggctc
     5281 gaccaggtct gggggtgagg ggtcggtgac ggtcaagcgg gcacagcgtt ccgaggacgg
     5341 gccggacggc gggcgggatc cgcagtcggc gcccccggcc acccggagcg gattcggccg
     5401 cgttctgatc gcggtgtacg ccgtgttcgc gatcgcggcc accggccgtt cggcgtacca
     5461 gctgagtgtg aaggcttccg atgctccgat cccctacacg ctgtcgctgg tggctgcgct
     5521 catctacgtc acggcgacgg tggcgctgtt ccgtggcgga ccgacctggc ggcgagtggc
     5581 gtgggtcgcg tgcagcatcg agctcatcgg cgtgctggtg gtcgggacgg tgagcgtgct
     5641 cgtcgagttt ccggatgcga cggtgtggag cgactacggg atcgggtacg gcttcgtccc
     5701 gctcgtgctg ccgatcttcg ggctctggtg gcttcgcaag accgcaccgc cacaggaggg
     5761 cggcttgcgt ccgtagcccc ggccgccccg ccagccgcct tcacggacgc aacaccacag
     5821 gaggcggctt gcgtccgtga aggcggctgt catcgcgatc gctttcacgg acgtcacgtc
     5881 gatagcgtgt ggccatgagc acgcacgaca cggagacggt cgagatcacc ggacacctca
     5941 tggacagcgg aatcctgtcc atggtcctca gtgacatccg cgactacggc ggcgaccacg
     6001 tcgtggagaa gttcgacctc gggcacgagg caaccgacat gtcgtacgcc cgcatcgtcg
     6061 tgagcgccca ggacgaggag tcgctgcagc ggatgctcat gcggctgcag acccgtggcg
     6121 tgaacatggt cgaccctggc gaggtgacgg tggtcgcagc cgaacaggac ggcgtcttcc
     6181 cggacggctt ctactccacg accaacctcc agacccgcgt gcgctaccaa ggtaagtggg
     6241 tgcaggtgtc caacccggag atggactgcg ggctcatcat cgacgacagc gcagacgaga
     6301 cgcgggtctc aacgctgccg atgtccgacg tgaaggcggg aat
//
LOCUS       DATBFZ010000533         4513 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_4867673, whole genome shotgun sequence.
ACCESSION   DATBFZ010000533 DATBFZ010000000
VERSION     DATBFZ010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 4513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4513
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4867673"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(192..983)
                     /locus_tag="VH419_16350"
     CDS             complement(192..983)
                     /locus_tag="VH419_16350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7045242.1"
                     /translation="MGTAFGSLWAVSDNGNRCLAGGPRKGYSLFRIDPEDGRVGDGIP
                     LHTDLSLYVTEVVGGEGAVWVATWTEPGTGDGPRYPHGVLSRVDPVAGEVTGRLGLNV
                     VEGHLAVGNGAVWVADTPRWGGAYILRKVDPSTMRIASRLRVGRRLGGVAVAFGSVWV
                     AADGSVLRIDPSTNRVVATITAGPDVGGFVIWRGLVWAGVPSDKGRGLTLGIDPSTNR
                     IVERERLLAFGSLAAFGSVWGTDDERELLWRTDRSDREPATQSPI"
     gene            2030..2200
                     /locus_tag="VH419_16355"
     CDS             2030..2200
                     /locus_tag="VH419_16355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7045243.1"
                     /translation="MRVRGVIMMVLGLALAAYSVPFVTGVAFAAFLLGVALVVFGVRL
                     ILRSGKARSEAT"
     gene            complement(2236..2637)
                     /locus_tag="VH419_16360"
     CDS             complement(2236..2637)
                     /locus_tag="VH419_16360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY7045244.1"
                     /translation="MILRVDAGSAVPPYEQVRRQIATMSATGVLPAGTRLPTIRQLSK
                     DLGLAGGTIARAYRELEAEGVIATHGRHGSFVAEANSAGRRERGDADLAAVAQDFAIR
                     VHQLGVEPRRAIEMAEEAFRAVQISQPARKA"
     gene            complement(2634..3560)
                     /locus_tag="VH419_16365"
     CDS             complement(2634..3560)
                     /locus_tag="VH419_16365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7045245.1"
                     /translation="MHARTWGSGLGLPEALLLVLPAVVFLAVVIRRAWVSDTAVERWC
                     VASGLQTSGESSRLARKFLETSRRFRACGAVLGFLVAISWEYAFGAAHIGWELPLALI
                     GYLLGAVAAETGANRPLRRPKEVARLVPRRVEDYVPPSLLRTQRVLGVAAFVVALVYA
                     SYTHSLASQWLPGQLAAFSRAYLLGSSLGLVPFGLLGLCVLALIEWAERSIVARPQPA
                     MDAERRALDDAMRATSARSIAGGGMALLLALVAIEVMVCAAGLDQPPSLARYLVAAVA
                     AALWLGSLVLWLRLSEPHAPESTRGMPPGLAA"
BASE COUNT          771 a         1534 c         1476 g          732 t
ORIGIN      
        1 tgcggggcaa atacccatag gaccccgttg ccagccacga acaccaagaa gaaaatgagc
       61 gcgatcaccc tccgtcccta cccagaatcg caagcttgcg ccgtgtcctc ggaggccggc
      121 cagggaggag gagcgtcggg ccccggatag ggtgacagac aagcgcccgg ggcaggtttg
      181 gctcccgggt gtcagatcgg cgattgggta gccggctcgc ggtcggacct atcggtccgc
      241 cacaggagtt cccgctcatc atcggtcccc cacactgaac cgaaggcggc aagggacccg
      301 aacgccagca accgttcccg ttccacgatc ctgttcgtgc tcggatcgat gccgagggtc
      361 aaaccccgac ccttgtcgct cggcactccg gcccagacga ggccgcgcca gatcacgaac
      421 cccccgacgt ccggtccggc cgtgatggtg gccacgacac ggttcgtcga cgggtcgatc
      481 cgtaggacag acccatcagc cgccacccac accgatccga atgccacggc aaccccgccg
      541 aggcgacgcc cgacacggag gcgggacgct atccgcatgg tcgaggggtc caccttcctc
      601 aagatgtagg cgccacccca acgtggtgtg tcggccaccc agaccgcgcc gttgccgacg
      661 gcgagatgac cctccaccac gttcaagcca agacgtcccg tgacctcgcc ggccacgggg
      721 tcgacccgac tgagcactcc atggggatag cgcggaccat ctccggtccc aggctccgtc
      781 caggtcgcga cccagaccgc gccttcgccg ccgacgacct cggtgacgta cagactgagg
      841 tcggtgtgca gcggaatccc atcgccgacg cggccgtcct ccggatcgat gcgaaacagt
      901 gagtatccct tgcgcggccc accggccagg cagcggttgc cgttgtcgct caccgcccag
      961 agagaaccga aggccgtgcc catgcctgct gtgctgcaca gcacgcgcga tccgattggc
     1021 tggttcgtat tggggtccac gcgctgaaga gagccatcgt cgcccatccg cacccagagg
     1081 agggagcctg ccgccgcgag tcgtgttgga tgcccgggca tgtgaatgcg cacgaaggcc
     1141 cagcgtgcgg gggcgggttc ctgggccgct ggcgcggccg ggtccgtgca ggcgccggcc
     1201 aagagtgcgg tcaataccag aggcacgctc cggcggagcc agcgtctgag gtcaatgggc
     1261 atctgcactg ttcatccaga caaccatcaa tcccttgccg ttaccagcaa acttcgtcga
     1321 ccgctgccaa tgctctcgac agacactccc ggtagtcctc gggccggtct gccagtgcag
     1381 ttggggcagc atgaacgctc gacgcgcagg gtatgagcac caaccgatgc ttgcgaccag
     1441 cgccgcgacg accgtcctct gtctcggcct ggccttcggt accgtcgctg ccctgttctg
     1501 gtttggagcg cgtgcccgaa tccagcctcc cgatccgaac tggcgtgacc accagccgcc
     1561 atcgaccaag gccccacgca gctactggat cacgtggtgg atcggcttcg ccctccttgt
     1621 ttcgatcgcc ctcgcggcat ccctggcgca tcagatctgg ctcgcgtggc ttgctttcgc
     1681 cgggtgggtt gtcacctacg ttgttcggct ggcgatcctc cggcaaaccc gccgagtctg
     1741 ggaggcgaaa tgagcgaacc gggcgcgagg aggcggtcgc cgaaccgcct ttccccggta
     1801 ttggtcgctg gcgaggcctc gactgctggc cgtttcgtac tggcgactgg gatggcacct
     1861 gctgttccca ggactcttcg ctgctctgtt cttggtgcgg tccgtccgcg ccgaccaaac
     1921 tggatgggca gtcctggtcg ggctcatgct gacgatcacg ctggctcagg tcgccttgtc
     1981 gctcgttctg aaggcaggag ccaggcgtgg acggtgagat gatcgtggaa tgagggtccg
     2041 cggcgtgatc atgatggtgc tcgggctcgc gctcgctgcg tattcggttc cgttcgtgac
     2101 gggggtggcc ttcgcggcct ttctcttggg cgtcgcactg gtcgtcttcg gcgtgcgact
     2161 gatccttcga agcgggaagg cacggagcga ggcaacctag gggtgttgtg ccgcagccgg
     2221 gccgctagag cgagatcagg ccttgcgagc cggctgcgaa atctgcacgg ccctgaaagc
     2281 ttcctccgcc atctcgatgg cccgccgcgg ctccacccca agctgatgaa cccggatcgc
     2341 gaaatcctga gcgacggccg ccaggtctgc gtcgcctcgc tcgcgacgac ctgcactatt
     2401 ggcttccgcg acgaaggacc catgccgccc atgcgtcgcg atgacacctt cggcttcgag
     2461 ctcccggtag gcgcgagcga tcgtgccgcc ggccaatccg aggtctttgg agagttgccg
     2521 gatcgtcggg agcctcgtcc cagccgggag cacccccgtt gccgacatgg tcgcgatttg
     2581 ccggcgaacc tgctcgtacg gcggcacggc cgagcccgcg tcgacgcgaa ggatcacgcc
     2641 gcgagccccg gcggcatgcc gcgtgtgctt tcgggggcgt gtggctcgga cagtcgcagc
     2701 cacagaacga gggagcccag ccacaaggcg gccgcgaccg cggccaccaa gtacctggcg
     2761 agcgatggcg gctgatcgag ccccgccgcg cacaccatca cctcgatagc cacgagtgcg
     2821 agcaggaggg ccatcccgcc gccggcgatg ctgcgggcgg aggtggcccg catcgcgtca
     2881 tcgagtgccc ggcgttcggc gtccatcgcc ggctggggtc gcgcgacgat cgaacgctcg
     2941 gcccactcga tgagcgcgag cacgcacagt cccagaagtc cgaaggggac cagaccgagg
     3001 gagctaccca acaggtacgc acggctgaac gccgcgagtt gtccggggag ccactgagac
     3061 gccagggaat gcgtgtagct cgcatagacg agtgccacaa cgaatgccgc caccccgagc
     3121 acgcgctgcg tgcggagcaa ggaaggaggg acgtaatcct ccacacgccg agggacgagt
     3181 cgagccacct ctttgggtcg ccggagcgga cgattggccc ccgtctctgc ggcgacggcg
     3241 cccaggagat acccgatcag agccaggggc agctcccacc cgatgtgggc cgctccgaat
     3301 gcgtactccc acgaaatggc gacgagaaac ccgagaacgg ctccgcaggc gcggaatctg
     3361 cggctcgtct ccaagaactt gcgagccagc cgactgctct cacccgatgt ttggaggccg
     3421 gatgccacgc accaccgttc caccgccgtg tccgacaccc atgcacggcg gatgaccacg
     3481 gccaggaaca cgaccgcggg gaggacaagg agaagagcct ccgggagtcc cagcccgctt
     3541 ccccacgtcc tcgcatgcat catcgtattg atacattgat acagaccgag gagtcaagcg
     3601 tcctctcgcg ttctcccggg agggctccgt cggctgctgg cggccgtgct cgactgaatc
     3661 ctcggggatg cgactccaat tcagtccggc gacggccggc aaaccgggac cggcggaggc
     3721 cggcggcacg gcgcgctggg gcagtccacg acagctcggg agggtgaagg tccggctgcg
     3781 ggattccgat aaatgggaca tgcgcgccgt cggtcgattg ttgccgagac cccttgctcc
     3841 cctgatgtgt ctcgtcctgt gcctggtcgc ttgctcggcg ccccacacca cgagtggttc
     3901 cggcccggct catccgcccg gcactccgtc ggtcgcaggc ggatcgccct cgcgccagcc
     3961 gaaccctctt tgctcccggc tgccggaggt cgacaagccc atagccagcg ttcttgcggc
     4021 gttccagctt cgagggtttc gaccgcggtc gcccacggcg cttctctcgc gaatcgccgc
     4081 tggaagaagt gccgcagcca acttggccac tgccgcagat gccgcaggga acgagcccgt
     4141 gtcaaatgac ttcatcgggt tggtggcccg tctggacacg ctctccgacg gcattgagga
     4201 cgcgggcgac ccgccccgct tcacaagaag gctgcgtcat ctcgctaacg gtgtcggggc
     4261 ggcgctgacg gcacctgcca tcgatctgca ttgcccattg acgttctgaa acagcagtga
     4321 gggactcctt aggagaggca ccgtgctcgt tcccatagac gactcggagt tcgattggat
     4381 tggagaggcc ggcggcctac cagagacgtt cgtccgaacg acctgcatcc tccacgcgat
     4441 gtgggaggac gtctcgatcg aggacaggac gctcacgtgg ggacggtggg aggagcagac
     4501 tggagtcgag gag
//
LOCUS       DATBFZ010000534         9160 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_5519944, whole genome shotgun sequence.
ACCESSION   DATBFZ010000534 DATBFZ010000000
VERSION     DATBFZ010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 9160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9160
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5519944"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="VH419_16370"
     CDS             <1..363
                     /locus_tag="VH419_16370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017933880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEY7045246.1"
                     /translation="ADGLGCEVGAIANSLVFDADGSPALILTSGAHRVDTTVTATTIR
                     VGSLGRANPDFVRRHTGQTIGGVAPLGHPEPIPTYLDKALAAYPTIWAAAGHSHAVFS
                     TTYTELRDLTGATEIDVE"
     gene            531..1055
                     /locus_tag="VH419_16375"
     CDS             531..1055
                     /locus_tag="VH419_16375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7045247.1"
                     /translation="MDDESDWRKQLVLGIGVLLLVAALVGSIIGVIAIKAADVAGIGA
                     TPAPDTSVDFPTFQPTTPSNDGSPTTPEEPTRSTPVKTPPAKGIRLTASPTQADTYQR
                     VNLTGTYQAPPGTSLQVQRKEGAAWVEFPTSATVSGGQFSTYIETGRPGKNLFRMLDS
                     GSGKTSNIVTIQIS"
     gene            complement(2047..4029)
                     /locus_tag="VH419_16380"
     CDS             complement(2047..4029)
                     /locus_tag="VH419_16380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012920484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HEY7045248.1"
                     /translation="MLAAAVGVSALALTASGLTFGAASSSATTSRHGSSGRDCLAAIP
                     AGPATHARQRVFAAASRTYHVPTSVLLGVSYMESRWDDHGASPSTSGGYGPMHLTDVA
                     VEAAGTAKGDGSVNRSTGPASLHTAATAADLTGFSVARLTSDDVANICGGAALLASYR
                     SSSHATGPASWYDAVAAYSGASSAADAREFVHRVFATVRHGEGRTTNDGQRVHVAAHP
                     TLRVPAARLPSAAKASTNPHIDCPRSLDCEWIPAPYEQYGPDPGDYGNHDIANREQDL
                     TIDYIVIHDTETSYDETLDLVQDPTYLGWNYTIRSSDGHVAQHMDAKDIGFQAGNWYI
                     NQHSMGIEHEGFAAQGASWFTENLYESSAKLVKYLAKKYTVKLDRAHIIGHDQVPGIT
                     PPLVAGMHWDPGPYWDWEHYFDLLGKPIARHGHGGHGHHALAASAFHKNDVVTVAPGF
                     DQNRQLVTGCDDPVDHPEAPCTPQGTNFVYLHTQPSEFAPLVTDVGLHPDGSPSTTFV
                     SDHGARVAAGMKLVVAGHSGDWLGVWYLGNIGWLESPRNHPTVVPSKGRVVQAKAGVA
                     EVPVYGRAYPEESAYEGTGVPVQAVVPLQYSIKAGQKYVLADDTIDTTYYYSTIFDVT
                     DPTNHIVVNGQDKYDEIWFGHRMFFVRVADITTSKH"
     gene            4367..4966
                     /locus_tag="VH419_16385"
     CDS             4367..4966
                     /locus_tag="VH419_16385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HEY7045249.1"
                     /translation="MPLVFTSGWGSGINAYAVIVMLNLLGRYAGVDAVPESLQSTTVL
                     AIAGGMFLVEFVTDKIPYVDSAWDTISTIIRPTIGAMLGYLIAGQADSLDHDQAAWYA
                     VMGGGVAMTSHLVKAGIRLAVNTSPEPVTNIGLSLGEDITVASVITLALYHPWLALAI
                     ATTLLVAGVLLLVVLIRLVRRGWRRWQSRRTSGSESARA"
     gene            4959..6437
                     /locus_tag="VH419_16390"
     CDS             4959..6437
                     /locus_tag="VH419_16390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7045250.1"
                     /translation="MPDDTPATGGDAEALDGWETYLSDPVRDRKVIVAFEVLAGMTGV
                     AAQLRRWGAQRPLLIADGRGTGPVPDSSEAEIVVLPTPNFESVTEQVRDRIRPAERLT
                     PDVTSAVESYDPEGTAVWWLNPVCLNEPLLERRVLGGRPRRQAALEDKLILDDLLDAT
                     GTERTPSRVVPAAYHDLTVGSDQLVEEATSCDATGNGTTTLTGVVWAGDNHEGPNGAG
                     DYVRWIRSPQQAREASAFFGRHCRRVRISLFLEGVPCSIHGIVLPDGVVVLRPVELVT
                     LREPETGLFVYGGMGTTWSPPEADATHMRDLTRRLGIHLRRAHGYRGGFCIDGVLTAD
                     GFRVTEVNTRFSGALARLHRAAPEAQLELVHLNALLDRDVGRSAAAIEDQALALLDAN
                     RFVDAMGLSRNVVIAEPEEVPVRVGEDRLEVAESDESTIGSVLAGPSALGSFFRFAAL
                     DGIVRPGERAAPLTVLLHEFADRTWGAGFHASLIAPDVRRRG"
     gene            6430..7779
                     /locus_tag="VH419_16395"
     CDS             6430..7779
                     /locus_tag="VH419_16395"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY7045251.1"
                     /translation="MADVVVVGAGLGGLAAAARLAKLGHRVTVLERNATAGGAIHRVE
                     KDGFGWDAGPSSTTLPAVLRDLFRKSGRPLERYVDLVLRTPARRHVFEDGSHVDLPTG
                     SRADQIRAVDAGLGTGSGAAWAAFVDAQGSLWDRLRLDVLDLPDGAARLADRDVARSL
                     GARTSLGTLLTKSLPDDRMRAMAGCHVELAGSTVQDAPAYAAVSCYVERSFGVWGVPA
                     GGLAAVTDALVTRLGERKVRLELGAEVTALLVTGGRVSGVTLADGTERRADVVVAAID
                     ARTVFGRLLPHGLNLPGRRLFGAATPAIPPSVTHVGLHGDVPALPDEVVLHGDPLLVL
                     TTCGSAPPDGHAWTVQRRGDAQEDVLVVLARRGIDVRDHVVTRLDRSADDLVRETGGS
                     PYGLAWPGWRAHVRRAAQSNPVPGLHLCGSSVHPGPGVPYVAWGAAHIATRIGPVQP"
     gene            complement(7902..8780)
                     /gene="metF"
                     /locus_tag="VH419_16400"
     CDS             complement(7902..8780)
                     /gene="metF"
                     /locus_tag="VH419_16400"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017933882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HEY7045252.1"
                     /translation="MAVPRIRELLATGERSYSFEFFPPKDDEGEARLWQAIRRLEPLR
                     PTFVSVTYGAGGSTRDRTIRVTERIASETTLTPVAHLTCVGHDRDELRHIIGAYADAG
                     VRNILALRGDPVAGPGTPWTPTPGGLTYASELVALVKTLGDFSVGVAAFPEGHREALS
                     LDADAQVLRAKADAGADFAVTELFFRPADYLAMVERANAAGVAIPIIPGIMPITNLRQ
                     IQRFAELSGREVPAEVVARFDAVVEPADVRHVGVEIATQMCDKLLSEGAPGLHFYTLN
                     RSTATREIYEALNIVV"
     gene            8914..>9160
                     /locus_tag="VH419_16405"
     CDS             8914..>9160
                     /locus_tag="VH419_16405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020390332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEY7045253.1"
                     /translation="MTVVQALDPVPAELRDRVAAELDEFVDGKQTALAPIGTELDALI
                     DAARKAVSGGKRLRPAFAYWGWRAAGGSADKTDAIIKA"
BASE COUNT         1398 a         3104 c         3186 g         1472 t
ORIGIN      
        1 gccgacgggc tcggctgtga ggtcggcgcg atcgccaact cgctcgtctt cgacgccgac
       61 ggctcccccg ccctcatcct cacctccggc gcccaccggg tcgacaccac ggtgaccgcg
      121 accaccatcc gtgtcggatc actgggtcgg gctaatccgg acttcgtacg ccggcatacc
      181 ggccagacga tcggtggagt ggcgccgctc ggacacccgg agccgatccc gacctacctg
      241 gacaaggcgc tggcggcgta cccgacgatc tgggccgccg cggggcactc gcacgcggtc
      301 ttcagcacga cgtacaccga gctgcgcgac ctcaccggtg ccaccgagat cgacgtcgag
      361 tagcgggtcg ctgtgacccg atcgtgactg ggttcaggca actcgggtgc ttcccggctc
      421 gtctgtcacc ataggactgt caccctcgaa aagggcgaaa ccagcccgct tcggcccggc
      481 aggggccact cgaacccacg taggtaccga caagacgagg acccttcccc atggacgacg
      541 agagcgactg gcgcaagcag ctggtcctcg ggatcggggt cctgctcttg gtggcggcgc
      601 tggtcggcag catcatcggc gtcatcgcga tcaaggcggc cgacgtggcc gggatcggtg
      661 cgacgcccgc cccggacacc agcgtcgact tcccgacgtt ccagccgacg actccctcga
      721 acgacggatc gcccacgacc cccgaggaac cgacccggtc aaccccggtg aagacgccac
      781 ccgcaaaggg catccggctc accgcctcac cgacgcaggc agacacgtac caacgggtca
      841 acctcaccgg cacctaccag gccccgccag gtacgtcgct gcaggtgcag cgcaaggagg
      901 gcgcggcttg ggtcgagttc ccgaccagcg ccaccgtcag cggcggccag ttctcgacgt
      961 acatcgagac cggtcggccc ggcaagaacc tgttcaggat gctcgactcc ggctccggga
     1021 agacgtccaa catcgtcacc atccagatca gctaggggtg gcggtttgcc tcggtagcca
     1081 tccaaacgga cgggtagcca ggcaggtctg ccttgggagg gatggatttg gctggttagc
     1141 catgcaaacc gccgctcggc aaggcggatc tttgtatcct cccgcctctg tgggccgcgc
     1201 ctgctaccgg tgcattcctc ccgcgtcaag ggtcgctacg cgatcgctga cgacggctcg
     1261 gtcagtgcag gtggcttatt gagccggacc cggcgactgc gtcgctcggg tccgtccatc
     1321 ccatgacccg cgagccttca tgcaccggtt cgggcaggct tgacgggcag gacttcgtcc
     1381 ggcccgtgca ccggcgcggc ccacccacct ccgaaaagcc acctacgtca ccaatcgggc
     1441 ccccgggtca ggggtttgcg atgtttacgg cccaaacggc gtgtaaacat cgcaaacctc
     1501 agatggacgg agcgggtctc ggatgtgcac atcgcaaaca tgtgacggtg gtggtttgcc
     1561 tcggtagcca tccaaacgga cgggcagcca ggcaggtctg ccttgggaag gatgggtttg
     1621 gctggtgagc catgcaaacc gtcgacggcg accgacggat gtccgaaacc cgccagtcaa
     1681 ccgctgggca aaagggacag aatgccagtg tccgtaaacc gcgagccaga cgcccggcaa
     1741 aagggacgga acgcggccga acagctcagg tggcagccag aattcccgct catatctgcc
     1801 tacccacctc agcggtggtc gacggagcgc cgggcgacca cgcccgccga ctgagcccct
     1861 tcccgcccac cgagcccctt cccgcccaca gcaatccgag gagagcccac gtctgctatg
     1921 cgacgggtgg ccttgccttg gcacctgccc cgccagcccg ccacggcgga cagccgggcg
     1981 accaacgccg accggccctt gctgcgtctg cagcaagggc cgggcgggag tcggacgcca
     2041 gagacgtcag tgcttcgagg tcgtgatgtc ggcgacccgg acgaagaaca tccgatggcc
     2101 gaaccagatc tcgtcgtact tgtcctgacc gttcaccacg atgtggttgg tcggatcggt
     2161 cacgtcgaag atcgtcgagt agtagtacgt ggtgtcgatc gtgtcgtccg ccagcacgta
     2221 cttctgaccg gccttgatcg agtactgcaa cggcacgacg gcctggacgg gaacacccgt
     2281 gccttcgtac gccgactcct ccgggtaggc acggccatag accggaacct cggcgacgcc
     2341 cgccttggcc tgcacgactc ggcccttcga cggcaccacg gtcgggtggt tgcgcggcga
     2401 ctcgagccag ccgatgttgc ccaggtacca gacgccgagc cagtcgccgg agtgtccggc
     2461 gacgacgagc ttcatcccag cggcgaccct ggccccatgg tcggagacaa acgtcgtcga
     2521 cggcgaaccg tccggatgca gaccgacgtc ggtcaccagc ggcgcgaact cactgggctg
     2581 cgtgtgcagg tagacgaagt tcgtgccctg cggcgtacac ggtgcctccg ggtggtcgac
     2641 cgggtcgtcg caaccggtga cgagctgacg gttctggtcg aagcccggcg cgaccgtcac
     2701 gacgtcgttc ttgtggaacg ccgacgcagc gagcgcgtgg tgtccgtgtc cgccgtgtcc
     2761 gtggcgggcg atcggcttgc cgagcaggtc gaagtagtgc tcccagtccc agtacggacc
     2821 cgggtcccag tgcatcccgg ccaccagcgg tggcgtgatg cccggcacct ggtcgtggcc
     2881 gatgatgtgg gcgcggtcga gcttgacggt gtacttcttc gccaggtact tgaccagctt
     2941 cgccgagctc tcgtagaggt tctcggtgaa ccagcttgct ccctgagcgg cgaagccctc
     3001 gtgctcgatg cccatcgagt gctggttgat gtaccagttg cctgcctgga agccgatgtc
     3061 cttggcgtcc atgtgctgcg cgacatggcc gtccgacgaa cggatcgtgt agttccagcc
     3121 caggtaggtc gggtcctgca cgaggtcgag ggtttcgtcg taggacgtct cggtgtcgtg
     3181 gatgacgatg tagtcgatcg tcaggtcctg ctcccggttg gcgatgtcgt ggttgccgta
     3241 gtcgccaggg tcaggtccgt actgctcgta cggcgccggg atccattcgc agtccagcga
     3301 cctcgggcag tcgatgtggg ggttggtgct cgccttcgcg gcggaaggca ggcgcgcagc
     3361 cggcaccctc agtgtcgggt gcgccgcgac gtggacgcgc tggccgtcgt tcgtcgtacg
     3421 gccttcaccg tgtcgcacgg tcgcgaagac acggtgcacg aactcgcgcg catcagccgc
     3481 ggaggaggcg ccgctgtacg ccgccacggc gtcgtaccag ctcgccggtc cggtggcgtg
     3541 tgacgatgac cggtaggacg cgagcagcgc cgcgccaccg cagatgttgg cgacgtcgtc
     3601 ggacgtcagg cgggcgacgc tgaagccggt gaggtcggcc gcggtggctg cggtgtgcag
     3661 tgaggccggg ccggtcgagc ggttgacgct gccgtcgccc ttcgctgtcc ccgcggcttc
     3721 cacggccaca tcggtgaggt gcatcggtcc gtatccaccg gacgtactgg gtgaggcgcc
     3781 gtggtcgtcc cagcgggact ccatgtacga gacgccgagc agcactgacg tcgggacgtg
     3841 gtaggtgcgt gaggcagcgg cgaagacgcg ctggcgggcg tgtgtggcgg gacccgctgg
     3901 gatcgcggcc agacagtcgc gaccggacga gccgtgtcgg ctggtggtgg ccgatgagga
     3961 cgcggcgccg aacgtgagcc ccgaggcggt gagcgccagg gcactcacgc cgacggcggc
     4021 agccagcatt cgacgttgtc gtgggagtcg gggtgatcgc catcgaggtg atcgggaagg
     4081 tgatgccgga gtacttctgg tgcgcatgca gatctcctgt gcagacgggt gcggtcatgc
     4141 agaggtcgcc gccgcagcag cgacgcctca ctcaaacaca ccggcgacga gtccgcaatg
     4201 accacgccga tgaatggtcc ttaccgcacc cgtgctgccg ggctcccgcg aattgcggga
     4261 agttccgcga tttcggggcc gaaaagcccg gagcttcccg caaatggcgg gagccaccgc
     4321 ccgcccgtag ggagtcgagc atccttagag ttgggccgtg gacgtcatgc cgctcgtgtt
     4381 cacctccgga tggggaagcg gcatcaacgc gtacgcggtc atcgtcatgc tgaacctgct
     4441 cggtcgctac gccggcgtgg acgccgtgcc ggagtccctc cagagcacca cggtgctcgc
     4501 gatcgcgggc ggcatgttcc tggtcgagtt cgtgacggac aagatcccgt acgtcgactc
     4561 cgcctgggac acgatctcga cgatcatccg gccgacgatc ggcgccatgc tgggctacct
     4621 gatcgccgga caggccgact cactcgacca tgatcaggcc gcctggtacg ccgtgatggg
     4681 tggcggggtg gccatgacgt ctcacctcgt caaagccggc atccgcctcg cggtgaacac
     4741 ctcgcccgag ccggtgacga acatcggtct ctcgctcggg gaggacatca cggtggcctc
     4801 cgtgatcacc ctcgccctct accacccctg gctggcactg gccatcgcga cgacgctgct
     4861 cgtcgccggc gtgctgctgc tcgtggtcct gatacggctc gtccgacgtg gctggcgacg
     4921 ctggcagtcc cgccgcacct ccggctcgga gtcggcccgt gcctgacgac acaccagcga
     4981 ccggcggtga cgccgaggct ctcgacggct gggagacgta cctctcggac ccggtgcgcg
     5041 atcgcaaggt catcgtcgcc ttcgaggtcc tggccggtat gacaggcgtc gcggcccagc
     5101 tacgccggtg gggagcccag cggccgctgt tgatagcaga cggtcgcggc accggcccgg
     5161 tcccggactc gagcgaggcc gagatcgtcg tcctccccac accgaacttc gagtccgtca
     5221 ccgaacaggt ccgcgatcgc atccggccgg cggaacggct cactccggac gtgacgtctg
     5281 ccgtcgagtc gtacgacccg gaaggcaccg cggtctggtg gttgaacccg gtctgcctca
     5341 acgagccact cctcgagcgt cgcgtgctcg gtgggcggcc acgccgccag gcggcgctcg
     5401 aggacaagct gatcctcgac gatctgctcg acgcgaccgg caccgaacgc acgccgagcc
     5461 gagtcgtgcc ggcggcgtac cacgacctga cggtgggcag cgaccagctg gtcgaggagg
     5521 cgacgtcgtg cgacgccacc ggcaacggga ccacgacgct caccggtgtg gtctgggccg
     5581 gcgacaacca cgaggggccg aacggtgccg gcgactatgt ccgctggatc cgttcacctc
     5641 agcaggcacg cgaggccagc gccttcttcg gccggcactg ccgacgggtg cggatcagcc
     5701 tgttcctcga aggggtcccg tgcagtatcc acggcatcgt gcttccggac ggcgtcgtcg
     5761 tgctgcggcc agtcgagctg gtcaccctgc gcgagccgga gacgggcctc ttcgtgtacg
     5821 gcggcatggg cacgacctgg tcgccgcccg aggctgatgc cacgcacatg cgcgacctga
     5881 cgcgacggct cgggatccac ctccgacgag cccacgggta ccgcggcggc ttctgcatcg
     5941 acggtgtgct caccgctgat ggcttccggg tcaccgaggt caacacgaga ttcagtggcg
     6001 cactcgcccg gctgcaccgg gcagcaccgg aggcgcagct ggagctcgtg catctcaacg
     6061 cgcttctcga tcgcgacgtc ggccggtccg ccgccgcgat cgaggatcag gcgcttgcgc
     6121 tgctggacgc caaccggttc gtcgacgcga tggggctgag ccggaacgtc gtcatcgcgg
     6181 agcccgaaga agtgcccgtg cgtgtcggcg aggaccggct ggaggtcgcc gagagcgacg
     6241 agagcaccat cggcagcgtg ctggccgggc cgtcagccct cgggtcgttc ttccggttcg
     6301 cggccctcga cggcatcgtc cgccccggag aacgagcggc accgttgacg gtgctgctgc
     6361 acgagttcgc agaccggacc tggggtgccg gcttccatgc gtcgctgatc gcgccggacg
     6421 tacgacgtcg tggctgacgt cgtcgtcgta ggcgccggac tcggcggttt ggcggccgcg
     6481 gctcggctgg cgaagctcgg ccaccgtgtc accgtgctgg agcgcaacgc caccgccggc
     6541 ggtgcgatac accgcgtcga gaaggacggc ttcgggtggg acgccgggcc gtcgtcgacc
     6601 acgctgccgg ccgtgttgcg cgacctcttc cgcaagtcgg gccggccgct cgagcggtac
     6661 gtcgacctgg tcctgcgtac cccggcccgg cggcacgtgt tcgaggacgg ctcccacgtc
     6721 gacctgccga cggggtcgcg tgcggaccag atccgcgccg tggatgcagg tctcggcacc
     6781 ggcagcggcg ctgcatgggc ggctttcgtc gacgcccaag gatcgctgtg ggaccgattg
     6841 cggctggacg tgctcgacct ccccgacggt gccgcgagac tcgccgaccg agatgtcgcc
     6901 cgctcgctcg gcgcgcgtac ttcgctcgga acgttgctca cgaagtcgtt gcccgacgac
     6961 aggatgcgag ccatggccgg ctgtcacgtc gagctggccg gctcgacggt gcaggacgca
     7021 ccggcgtacg ccgcggtcag ctgttacgtc gagcgcagct tcggggtctg gggtgtcccg
     7081 gccggggggc tggcggcggt gaccgacgcg ttggtgacca ggctcggcga gcgcaaggta
     7141 cggctcgagc tcggcgccga ggtcacggcg ctgctcgtca ccggtgggcg cgtgtcagga
     7201 gtcacgctgg ctgacggcac cgagcgtcgt gctgacgttg tcgtcgccgc gatcgacgcg
     7261 cggaccgtgt tcggccgcct ccttccccac ggtctgaacc tgcctggacg gcggttgttc
     7321 ggggccgcaa ctccggccat cccgccgagc gtgacgcatg tcggtctgca cggcgacgtc
     7381 ccggcccttc cggacgaggt ggtgctgcac ggtgacccgc tgctggtgct caccacgtgt
     7441 ggcagcgcgc cgccggatgg acatgcctgg acggtgcagc gtcgcggcga tgctcaggag
     7501 gacgtgctgg tggtcctcgc ccgtcgcggt atcgacgttc gcgaccacgt cgtgacgcga
     7561 ctcgaccggt ctgctgacga cctggtccgc gagacgggcg ggtcgccgta cgggctggcg
     7621 tggcccggct ggcgcgcgca cgtcaggcgt gctgcccaga gcaacccggt gccgggcctc
     7681 cacctctgcg ggagctccgt ccaccccggc cccggcgtcc cgtacgtcgc ctggggcgcg
     7741 gcccacatcg ccacccgcat cggccccgtc caaccgtgag ttgtggttcg ttcaaccgtg
     7801 agttgtggtt cgttcaaccg tgagttgtgg ttcgtcgaac cgtgagttgt ggttccttga
     7861 gtcgtgggtt gcggttggtg gaggtcccgt agcgctcacc gctacacgac gatgttgagg
     7921 gcctcgtaga tctcgcgggt ggccgtcgag cggttcagcg tgtagaagtg gaggcccggc
     7981 gcaccttcgc tcagcagctt gtcgcacatc tgggtggcta tctccacgcc cacgtgacgc
     8041 acgtccgccg gttcgacgac cgcgtcgaag cgcgcgacca cctcggcagg cacctcgcgg
     8101 cccgagagct cggcgaaccg ctggatctgc cgcaggttcg tgatcggcat gatgccgggg
     8161 atgatcggga tcgcgacgcc ggccgcgttc gcgcgttcga ccatggcgag gtagtcggcg
     8221 gggcggaaga acagctcggt gaccgcgaag tcagcgccgg cgtcggcctt ggcccggagc
     8281 acctgcgcat cggcgtcgag cgagagcgcc tcacggtgcc cctcggggaa cgcggccaca
     8341 ccaacgctga agtcgcccag cgtcttcacg agcgcgacca gctcgctggc gtacgtcaag
     8401 ccgccgggcg tcggcgtcca cggcgtaccg ggtccggcca ccggatcacc acgcagagca
     8461 aggatgttgc gaacgcctgc atccgcgtac gcaccgatga tgtgccgcag ctcgtcgcgg
     8521 tcgtggccga cacaggtcag gtgggcaacc ggagtcagcg tcgtctcgct ggcgatccgc
     8581 tcggtgaccc gaatggtgcg gtcgcgtgtc gagccgccgg cgccgtacgt cacggagacg
     8641 aacgtcgggc gcaacggttc gagcctgcgg atcgcctgcc acagtcgggc ctcaccctcg
     8701 tcgtccttcg gcgggaagaa ctcgaaggag tacgagcgct ctccggtcgc cagcagctcc
     8761 cggatcctcg gcacggccat gccgacactg tagggcttgt ctcccggcga cgaggtactc
     8821 cgaccgagca catccgacgt gtcgatcgtc cactgtcgag tcggcgtgtc atggggagcg
     8881 ttggcgcctc ggcgggcggg taatgtcgca ggcgtgaccg tcgtgcaggc cctcgacccg
     8941 gtgccggctg agctgcgcga tcgggtcgcg gccgagctcg acgagttcgt cgacggcaag
     9001 cagaccgcac tcgcaccgat cggcaccgag ctcgacgcgc tgatcgacgc tgctcggaag
     9061 gcggtgtccg gcggcaaacg actgcgtccg gcgttcgcgt actggggatg gcgggcggca
     9121 ggcggctctg ccgacaagac cgacgcgatc atcaaggccg
//
LOCUS       DATBFZ010000535         6862 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_4868889, whole genome shotgun sequence.
ACCESSION   DATBFZ010000535 DATBFZ010000000
VERSION     DATBFZ010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 6862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6862
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4868889"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2518
                     /gene="topA"
                     /locus_tag="VH419_16410"
     CDS             <1..2518
                     /gene="topA"
                     /locus_tag="VH419_16410"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013161892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEY7045254.1"
                     /translation="VYVVPRDKKSQITKLRSLLKDADELYLATDEDREGEAIAWHLYD
                     ELKPRIPVHRMVFHEITPEAIATAVANPREIDDDLVEAQEARRILDRLSGYEISPVLW
                     KKVMSGLSAGRVQSVATRLVVDRERERMAFRAASFWDLEATFDAGEGKDPQTFPAKLT
                     GVDGRRVAQGRDFSSTGVLRDGADSLDRVHLDEAAAAALAEALRDQRFAVRSVESKPS
                     RRSPYAPFRTTTLQQEASRKLGYGAARTMQIAQRLYENGFITYMRTDSITLSDTAVSA
                     ARAQVAELYGREYLPDSPRTYQSKVKNAQEAHEAIRPAGEQFRTPAQTGLSGDEFRLY
                     ELIWMRTVASQMRDAEGRSVSVRIGATASTGEACEFSASGRVITFYGFLKAYVEGRDD
                     PDADRDDRETRLPDLAEGNPLTVLGLGPAGHETRPPARYTEASLVRELEEREIGRPST
                     YASIIGTILNRGYVYKKGTALVPAWLAFAVVRLLERHFARLIDYNFTAGMEDVLDEVA
                     AGRRDSTSELAEFYFGSGRHEGLKTIVSELGEIDARELSTFPIGDGVVLRVGRYGPYV
                     EDGHGNRANVPEDLPPDELTLDRAHELLANPAGVEQELGTDPETGRTIVAKSGRFGPY
                     VTEVLENDAPKSAKPRTGSLFKSMSLDAITLEQALTLLTLPRVVGVDPGSEEEITAQN
                     GRYGPYLKKGTDSRSLDSEEQLLTITLDEALAIYAQPKQRGRRAAAAPLKELGKDPAT
                     DLPVVVRDGRFGPYVTDGEINATLRKADAVDTITIERAAELLAEKRAKGPAPTKTAAK
                     KTATKKTAAKKTAAKKTAAKKTTAKKSTAGKASAKKTATK"
     gene            2630..4363
                     /locus_tag="VH419_16415"
     CDS             2630..4363
                     /locus_tag="VH419_16415"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY7045255.1"
                     /translation="MVAAVALVAWNGLRSDGDSAPEGRAETGELGDGAFDPDRQGPAA
                     PVQGAVEGGTVTVSTAGTYGLKSWDPTAAYGGLPDFVLRGLVTRSLTQYVYDPQQGSS
                     VLVPDLATDLGTPNADFTEWTYTIRSGVRWEDGTEVTADDVAFGVKRSFEPDNGAFSS
                     VFAPAPFSRDFFLDGDTYQGPYKSGSQYSGVVVQGNTLTLKMRKPFPDMPYWAAYPAM
                     GPIPESGSNPATYGAHPLATGPYKVAGVAGRKTLTLVRNDEWDPDTDPGRRQYPDRYV
                     FHFGAPSDHVAATILDGSAVGQTAISYDSPSPDDMRMAAGSGLLTTDIPDLCTHYWTF
                     DVRTFDDIRVRRAVGYAFPYGEALSPFADVLDVTAEPGSSILPPGLPGRQRYSALPRT
                     SSESGVNQAKALLKAAGYAPGEFVVRWPYTEDDPEQKASIDAIVEALESAGFDVRPVA
                     VPVAPGIGEWAYDPQAPIDLRGLAWCGDYPSGSSWLPALFSSDGQTPWALPPSMVKLV
                     DAESERIGALPIDQQLTEWGALDKKVMREYFPALAVSYKRWPMLHGSRIGGMNTDWGE
                     PTWKDIYVSPR"
     gene            4389..6395
                     /gene="tmk"
                     /locus_tag="VH419_16420"
     CDS             4389..6395
                     /gene="tmk"
                     /locus_tag="VH419_16420"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020393368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEY7045256.1"
                     /translation="MPSVRPLPPTRGVLSIPSFRKLWIGLGLSSLGDWLGLLALTAMA
                     SALAGDGYAQQNFAIAGVLFLRVLPAVVLGPIAGYIADRLDRRWTLIVGDVLRGLIFV
                     TIPIVGTLEWVFVATVLIEAVTLVWLPAKDATIPNLVPRDKLEAANQVSIATTYGSAL
                     PAAAIFVLLSLSTKGLNSALGWLDAPVTLSLVFNAASFVVSGLVIATLHDIPPGPALA
                     PEDRRGMVAVIHEGWAYVARTPLIRGLVIGIAGAFAAGGVVVGLARVYVADLGGGDPG
                     YGLLFAAVFTGLALGMWRGPRLLVGMTRRRLFGFALISAGVLLAAIAVIANLVVVTFL
                     TVVLGFFSGAAWITGYTLLGLEVEDSMRGRTFAFVQTLIRLVLALVLAAAPLVAGLIG
                     THSWTINDDAELDYNGAAITMFASSLLMIAVGVLAYRQMNDRPGATIRSELKRSLMGE
                     QGSYAEHGVFVALEGGEGAGKSTQAQLLRDWLEHRGYDVVLTHEPGDSAVGEQLRQIL
                     LSPETGDLDHRAEALLYAADKAEHVEKVVRPALARGAVVITDRYVDSTLAYQGAGRDL
                     LPREVERIARWATSDLRPHLTVLLDVAPELGLGRFDTVDRIEAESQAFHARVRESFLE
                     LAAADPEHYLVIDAGLPREQIAAVVRDRLAPVLPLADRRPGVDA"
     gene            6392..>6862
                     /locus_tag="VH419_16425"
     CDS             6392..>6862
                     /locus_tag="VH419_16425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012918265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY7045257.1"
                     /translation="MSVWDDLVGQRDVVDRLRASAESAARAMLGEATSGMTHSWLFTG
                     PPGSGRSTAARAFAAALQCERGGCGECLACRTSLAGSHADVTVIATEQSQLKVGQIRE
                     YVRQAALAPAGRRWQVLIVEDADRLNDPAADALLKSIEEPPPRTVWLLCAPTVED"
BASE COUNT         1082 a         2368 c         2340 g         1072 t
ORIGIN      
        1 ggtctacgtc gttcctcgtg acaagaagtc acagatcacc aaactgcggt cgctgctcaa
       61 ggacgccgac gagctctacc tcgcgaccga cgaggaccgc gagggcgaag cgatcgcctg
      121 gcacctgtac gacgagctga aaccacgcat ccccgtccac cggatggtgt tccacgagat
      181 cacgccggag gcgatcgcca cggccgtggc caacccacgc gagatcgacg acgacctggt
      241 cgaggctcag gaggcacgcc ggatcctcga ccgcctctcc ggctacgaga tctcgccggt
      301 gctgtggaag aaggtgatga gcggactgtc cgccgggcgg gtccagtccg tcgccacccg
      361 gctcgtcgta gaccgcgagc gcgagcggat ggcgttccgg gcggcgtcct tctgggacct
      421 cgaggccacc ttcgacgccg gcgagggcaa ggacccgcag accttccccg cgaagctgac
      481 cggggtcgac gggcggcgcg tggcccaggg ccgcgacttc tcctcgaccg gagttctccg
      541 ggacggcgcc gacagcctcg acagagttca cctcgacgag gccgccgccg ctgccctcgc
      601 cgaggcgctg cgcgaccagc gtttcgccgt ccgctcggtc gagtcgaagc cgtctcgccg
      661 atcgccgtac gcaccgttcc gtacgacgac gctgcagcag gaggcgtccc ggaagctggg
      721 ctacggcgcg gcacggacga tgcagatcgc gcagcggctc tacgagaacg gcttcatcac
      781 ctacatgcgc acggactcga tcacgttgtc cgacaccgcc gttagcgccg cacgcgcgca
      841 ggtcgccgag ctctacggac gcgagtacct gcccgactcg ccccgcacgt atcagagcaa
      901 ggtcaagaac gcccaggaag cccacgaagc gatccgtccc gcgggagaac agttccgcac
      961 tccggcgcag accgggctgt ccggtgacga gttccgtctc tacgagctga tctggatgcg
     1021 gacggtcgct tcgcagatgc gtgacgccga aggtcgttcg gtctccgtcc gcatcggcgc
     1081 caccgcatcc accggcgaag cctgcgagtt ctccgcgagc ggacgcgtca tcacgttcta
     1141 cggcttcctg aaggcctatg tcgaagggcg tgacgacccc gacgcggacc gggacgaccg
     1201 cgagacgcgg ctgccagacc ttgccgaagg caacccgctc accgtcctcg gactgggccc
     1261 agccgggcat gagaccagac cacccgcgcg ctacaccgag gcctctctcg tgcgcgagct
     1321 ggaggaacgc gagatcggac gcccgtcgac gtacgcgtcg atcatcggga cgatcctcaa
     1381 ccggggttac gtctacaaga agggcaccgc gctggtgcct gcctggttgg ccttcgccgt
     1441 ggtacggctg ttggagcggc acttcgcccg gctgatcgac tacaacttca cggccgggat
     1501 ggaggacgtc ctcgacgagg tggcggccgg ccggcgtgac tcgaccagtg agctggcgga
     1561 gttctacttc ggctcaggcc ggcacgaagg tctgaagacg atcgtgagcg agctcggtga
     1621 gatcgacgcg cgcgagctgt cgaccttccc gatcggtgac ggcgtcgtcc tgcgggtcgg
     1681 ccggtacggc ccgtacgtcg aggacggcca cgggaaccgg gccaacgtcc ccgaggacct
     1741 gccgccggac gagctgacgc ttgacagggc gcacgagctg cttgccaacc ccgcgggcgt
     1801 ggagcaggag ctcgggaccg acccggagac cggtcggacg atcgtcgcca agagcggccg
     1861 cttcggcccg tacgtcaccg aagtgctcga gaacgacgcc ccgaagagcg ccaaaccgcg
     1921 gaccggcagc ctgttcaagt ccatgtcgct cgacgcgatc accctcgagc aggcgctgac
     1981 cctgctcaca ttgccacggg tcgtcggcgt cgaccccggg tcggaggaag agatcaccgc
     2041 gcagaacgga cgctatgggc cgtacctgaa gaaagggacc gactcgcgct ccctggattc
     2101 cgaggagcag ctgctgacga tcacgctcga cgaggcactg gcgatctacg cccagccgaa
     2161 gcagcgcggt cggcgcgccg cggcagcgcc gctcaaggag ctcggcaagg acccggccac
     2221 ggatctccct gtcgtcgtca gggacggccg gttcggcccg tacgtcaccg acggggagat
     2281 caacgccacg ttgcgcaagg ccgacgcggt cgacacgatc acgatcgagc gagctgcgga
     2341 gctactggcc gagaagcggg cgaagggacc ggccccgacg aagaccgccg ccaagaagac
     2401 cgccaccaag aagaccgccg ccaagaagac cgccgccaag aagaccgccg ccaagaagac
     2461 cactgcaaag aagtccactg ctgggaaggc gtccgccaag aagactgcca cgaagtaggc
     2521 gcgcgccctc agccgtcgtg gtggacggca tcctgagccg agagcacact cgattgtggc
     2581 ccgtcgtacg aggctgggga ctgcggtcgc cgcgggtgct ggagtggcgg tggtggccgc
     2641 ggtcgcgctg gtggcgtgga acggtctgcg ttccgatggc gattcggcac cggaaggtcg
     2701 cgccgaaacc ggcgagctgg gggatggggc gttcgacccc gaccggcaag gtcccgccgc
     2761 gccggtgcag ggcgccgtcg agggagggac cgtgacggtg tcgaccgccg gcacgtacgg
     2821 gctcaagtcc tgggacccga ccgccgcgta tggcgggctt ccggacttcg tcctccgggg
     2881 gttggtcacc cgctcgctga ctcagtacgt ctacgacccg cagcagggct cgtcagtgct
     2941 ggttcccgac ctcgcgaccg acctcggaac ccccaacgcg gacttcacgg agtggacgta
     3001 cacgatccgc agcggtgtgc gttgggaaga cgggaccgag gtgaccgccg acgatgtcgc
     3061 gttcggtgtc aaacgctcgt tcgagcccga caacggcgcg ttcagctccg tgttcgcgcc
     3121 ggccccgttc agccgcgact tcttcctcga cggcgacacc taccaaggtc cctacaagtc
     3181 gggcagccag tactccggcg tggtcgtgca aggcaacacg ttgacgctga agatgcggaa
     3241 gcctttcccg gacatgccct actgggctgc ctacccggcg atgggtccga tcccggagag
     3301 cggcagcaac ccggcgacgt acggggcgca ccccctggcc acgggtccgt acaaagtcgc
     3361 gggcgtcgct gggcggaaga cactgacgct ggtccgcaac gacgagtggg atcctgacac
     3421 agacccaggc cgccgccagt acccagaccg ctacgtcttc cacttcggtg cgccatccga
     3481 ccacgttgcc gcgacgatcc tcgacggctc cgccgtgggc cagaccgcga tctcgtacga
     3541 cagcccgtcc ccggatgaca tgcgaatggc tgcgggctcg ggcctgctga cgacggacat
     3601 cccggacctg tgcacccatt actggacctt cgacgtccgc accttcgacg acatcagggt
     3661 tcgccgagcc gtcggctacg cgttccccta tggcgaggca ctcagcccgt ttgcggacgt
     3721 cctggatgtc accgccgagc cgggttcgtc gatcctgccg ccagggttgc ctggacgaca
     3781 gcgctacagc gcgctcccac gcacctcgtc ggagagtggg gtcaaccagg ccaaggccct
     3841 cctgaaggcc gcaggatatg caccgggtga gttcgtggtg cgatggccct acacagagga
     3901 cgatcctgag cagaaggcgt cgatcgatgc gatcgtcgag gcgctcgaat ccgcgggttt
     3961 cgacgtcagg ccggttgcgg tgccggtcgc ccccgggatc ggtgagtggg cctacgaccc
     4021 gcaggctccg atcgacctcc ggggccttgc gtggtgcggg gactacccgt cgggctcgtc
     4081 gtggcttccg gcgttgttca gctcggacgg ccagaccccc tgggcccttc caccgtcgat
     4141 ggtgaaactg gtcgacgccg agagcgagcg catcggcgcg ttgccgatcg accagcagct
     4201 gacggagtgg ggcgccctgg acaagaaggt catgcgcgag tacttcccag ccttggccgt
     4261 ctcctacaag cggtggccga tgttgcacgg gtcgaggatc ggcggcatga acaccgactg
     4321 gggcgagccg acctggaagg acatctacgt gtccccccgc tgacggggtc acagcctagg
     4381 cttggcccgt gccgagtgtc cgaccgttgc cgccgacgcg gggtgtgctc tcgatcccct
     4441 cgttccggaa gctgtggatc gggctcgggc tctccagcct cggcgactgg ctgggtctgc
     4501 tcgcgctcac ggcgatggca agcgcgctgg cgggtgacgg ctacgcccag cagaacttcg
     4561 ccatcgccgg tgtgctgttc ctccgcgtcc tcccggccgt cgtcctcgga ccgatcgcgg
     4621 gatacatcgc cgaccggctc gaccgcaggt ggacgctgat cgtcggtgac gtcctgcgcg
     4681 gtctgatctt cgtgacgatc ccgatcgtcg gcacgttgga gtgggtgttc gtcgcgaccg
     4741 tgctgatcga ggcggtcacg ctcgtctggt tgcccgcgaa ggacgcgacg atcccgaacc
     4801 tggtgccgcg cgacaagctc gaagccgcca accaggtgtc gatcgccacg acctacggct
     4861 cggcgttgcc cgccgccgcg atcttcgtcc tgctgtcgct ctcgacgaag gggctgaact
     4921 cggcgctcgg ctggctggac gcaccggtca ccttgtcgtt ggtgttcaac gctgcgtcgt
     4981 tcgtcgtgtc gggcctcgtg atcgccacgc tgcacgacat acctcccggc cccgccctcg
     5041 ctcccgagga ccgacgcggc atggtcgccg tcatccacga agggtgggcg tacgtcgcgc
     5101 ggactcccct gatccgcggg ttggtcatcg gcatcgcagg tgcattcgcc gcgggtggtg
     5161 tggtcgtcgg gctcgcgcgt gtgtacgtgg ccgaccttgg tggcggagac cccggttacg
     5221 gcctgttgtt cgcggccgtg ttcaccggat tggcactcgg gatgtggcgg ggcccgcggc
     5281 tgctcgtcgg catgacgcgg cgcaggttgt tcggcttcgc gctgatctcg gccggcgtcc
     5341 tcctggccgc catcgcggtc atcgccaacc tcgtcgtcgt cacgttcctc accgtcgtcc
     5401 tggggttctt ctcgggcgct gcctggatca ccggctacac actgctcggg ctcgaggtcg
     5461 aggactcgat gcgtggacgg acgttcgcgt tcgtgcagac actgatccgg ctggtgcttg
     5521 cgctcgttct ggcggctgcc ccgctggtcg ctggtctgat cggtacgcac agctggacca
     5581 tcaacgacga cgccgagctc gactacaacg gtgctgcgat caccatgttc gcctcgtcgc
     5641 tgttgatgat cgctgtcgga gtgctggcgt accgtcagat gaacgaccgg cccggcgcca
     5701 cgatccgcag cgagctgaag aggtccttga tgggtgagca gggctcgtat gccgagcacg
     5761 gcgtcttcgt cgcgctcgag ggtggcgaag gggccggcaa gtcgacgcag gctcagctgc
     5821 tccgcgactg gctcgagcac cgcgggtatg acgtcgtgct cacccacgag cccggtgact
     5881 ccgccgtcgg cgagcagcta cgccagatcc tgctctcgcc ggagacgggc gacctcgacc
     5941 atcgcgccga ggcgctgttg tacgcggcgg acaaggccga gcacgtcgag aaggtcgtgc
     6001 gcccggcact cgcccgaggc gcggtcgtca tcaccgaccg ctacgtcgac tccacacttg
     6061 cctaccaggg tgcgggacgc gacctgctcc cgcgcgaggt cgagcgcatc gcccgatggg
     6121 ccacgtcgga tctgcggccg cacctcaccg tgctgctcga cgtcgcaccc gagctcgggc
     6181 tgggcaggtt cgacaccgtc gaccggatcg aggcggagtc acaggcgttc cacgcacgcg
     6241 tgcgtgagtc cttcctcgag ctcgccgcag ccgaccccga gcactacctc gtcatcgacg
     6301 ccgggctgcc gcgcgaacag atcgcggccg ttgtccggga ccgcttggca ccggtgcttc
     6361 ctcttgccga ccgtcggccg ggggtcgacg catgagcgtc tgggacgatc tcgtcggaca
     6421 gcgcgacgtg gtcgaccggc tccgcgccag cgcagagtcc gctgctcgcg cgatgctcgg
     6481 tgaggcgacc agcggcatga cccactcctg gctgttcacc gggccgcccg gatccggccg
     6541 atcgaccgcg gcgcgggcct tcgcggctgc gttgcagtgc gagcgtggtg gttgcggaga
     6601 atgcctggcg tgccggacct ccctcgccgg ttcgcacgcc gacgtgacgg tgatcgcgac
     6661 cgagcagagc cagctcaagg tcgggcagat ccgcgagtat gtccgacagg ccgcgctggc
     6721 gccggccggc cgccgctggc aggtgctgat cgtcgaggac gccgaccggc tcaacgaccc
     6781 cgccgcggac gccctcctca agagcatcga ggagccgccg ccgcggaccg tctggctgct
     6841 ctgcgcaccg accgtcgagg ac
//
LOCUS       DATBFZ010000536         1558 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_4538991, whole genome shotgun sequence.
ACCESSION   DATBFZ010000536 DATBFZ010000000
VERSION     DATBFZ010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4538991"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1558
                     /locus_tag="VH419_16430"
     CDS             <1..>1558
                     /locus_tag="VH419_16430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEY7045258.1"
                     /translation="HLGPVLTGYDNATEVIVEGGYIRAVQDVRGKYGSEGDYVVNRPL
                     HGPQNPTPVDHATDTYDTIDWLVKNVPESNGKVGIIGISYDGFTPLMALVNPHPALKV
                     SVPMNPMVDGWMGDDWFHNGAFRQQGIGYIFEQEADRKNKEKFWRTHYDDYDVFMSAV
                     SAGELGRRHGMDVIGFWRKILDHPSYDAFWQDQAMDKILAAQPLKVPVLLVHSLWDQE
                     DIYGATAVYKALKPRDTANDKVFMVLGPWHHGQAIHDGSTLGPLRFEQNTALQFRRDV
                     LAPFLAQYLKDGAPKADIATVTAFETGTNAWRRLPSWPAGCASGCTVKGTPIYLTQGP
                     GLSRIAPAAGGREYEEYVSDPARPVPFISRPVQASGARWADWLVTDQREASGRTDVLT
                     FVSEPLTAPVKISGQPIANLVASTSGTDSDWVVKVIDVYPDEVGSQPEMGGYQLMVSA
                     DIFRGRYRESLDTAKPIPAGQPLLYKFALPNANHVFLPGHRMMVQVQSSWFPLYDRNP
                     QTFVPNIFWAK"
BASE COUNT          272 a          528 c          528 g          230 t
ORIGIN      
        1 gcacctcggc ccggtgttaa ccggttacga caacgcgacg gaggtgatcg tcgagggggg
       61 atacattcgg gccgtccagg acgtgcgcgg caagtacggc tcggaggggg attacgtcgt
      121 gaaccggccg ctgcacgggc cgcagaaccc gacgcccgtc gaccacgcga ccgacacgta
      181 cgacacgatc gactggctcg tgaagaacgt ccccgagtcg aacggcaagg tcggcatcat
      241 cggcatctcg tacgacggct tcacgccgct gatggctctc gtgaacccgc acccggcgct
      301 caaggtctcg gtgccgatga accccatggt cgacggatgg atgggcgacg actggttcca
      361 caacggcgcc ttccggcagc agggaatcgg ctacatcttc gagcaggagg cggaccgcaa
      421 gaacaaggag aagttctggc gcacgcacta cgacgactac gacgtgttca tgagcgcggt
      481 ctccgcgggt gagctcggcc ggcgccacgg gatggacgtg atcggcttct ggaggaagat
      541 cctcgaccat ccgagctacg acgcgttctg gcaggaccag gcgatggaca agatcctcgc
      601 ggcgcagccg ctcaaggtcc cggtgctgct cgtccacagc ctgtgggacc aggaggacat
      661 ctacggcgcg acggcggtct acaaggcgct gaagcccagg gacactgcca acgacaaggt
      721 gttcatggtg ctcggcccgt ggcaccacgg ccaggcgatc cacgacggct cgacgctggg
      781 accgctgcgc ttcgagcaga acaccgcgct ccagttccgg cgcgacgtgc tcgcgccgtt
      841 cctcgcgcag tatctgaagg acggcgctcc gaaggcggac atcgcgaccg tgacggcgtt
      901 cgagaccggc acgaacgcgt ggcgccgcct gccgtcctgg ccggccggct gcgcgtccgg
      961 ctgcacggtg aagggaacgc cgatctacct gacccagggc ccggggctga gccgcatcgc
     1021 gcccgccgcg ggcggccgcg agtacgagga gtacgtctcg gatcccgcga ggccggtccc
     1081 gttcatctcc cgtcccgtcc aggcgagcgg cgcgcggtgg gccgactggc tcgtcaccga
     1141 ccagcgcgag gcttcggggc gcaccgacgt gctgacgttc gtctcggagc cgctgaccgc
     1201 gccggtgaag atcagcggcc agccgatcgc gaacctcgtg gcttccacga gcgggacgga
     1261 ctcggactgg gtcgtcaagg tcatcgacgt ctatcccgac gaggtcgggt cgcagccgga
     1321 gatgggcggc tatcagctca tggtctccgc cgacatcttc cggggccgct accgcgagag
     1381 cctcgacacc gcgaagccga tccccgcggg ccagccgctg ctctacaagt tcgcgctgcc
     1441 gaacgcgaac cacgttttcc tgccgggcca ccgcatgatg gtccaggtgc agtcgagctg
     1501 gttcccgctc tacgaccgca acccgcagac gttcgtcccg aacatcttct gggcgaag
//
LOCUS       DATBFZ010000537         2588 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_4540603, whole genome shotgun sequence.
ACCESSION   DATBFZ010000537 DATBFZ010000000
VERSION     DATBFZ010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2588
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4540603"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..512
                     /locus_tag="VH419_16435"
     CDS             <1..512
                     /locus_tag="VH419_16435"
                     /inference="COORDINATES: protein motif:HMM:NF025687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4386 family protein"
                     /protein_id="HEY7045259.1"
                     /translation="GDPARVPMTVGVLLMMVAGLAVVGIGVLMYPVLKEVDPRLAGWY
                     PAMRITEFAVSAACGVYLLAKLEVVPNYLLWVYVPTGVGGIVLTYLLFVSRLLPRAIA
                     LLGLVGYGLLTIGVPLDLLGILDMSAGAGLVLVLPGGLFELVFLPSWLIAKGFSAPRS
                     SEDSGSRVQ"
     gene            796..1746
                     /locus_tag="VH419_16440"
     CDS             796..1746
                     /locus_tag="VH419_16440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7045260.1"
                     /translation="MAESQSAILSRTKSKGSKLSSDTRSILTAALALPVLTSLSLSSP
                     SAGLTSATAAPREPASHVRHVPVPAPVVHVHVTVKKHRIIASKSGFRPGNTVVDLSSE
                     GGGIAAIQLLRLRSGYTFAEFQADVESDNIQALRRIDRDAIFYGGMPVYERRASHFGA
                     RLDAGRYFLIDFDHPRIGRLLIEGAPERRSLPRSTGSINMVLDHQQHRFQTPRRLPRS
                     GWLRQTNHTDEPHFMDMIRIKSSTTRKQVRRFYSGQGPEDPSWVLHNYSGTFLISPGR
                     SVVWRYAFPRGKYLEACYWPSREDGTTHAEMGMWNFTTLE"
     gene            1933..2322
                     /locus_tag="VH419_16445"
     CDS             1933..2322
                     /locus_tag="VH419_16445"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY7045261.1"
                     /translation="MDLFKTPQIVLFCRDVSRTAQFYERLGFEEAFRTPADDPPIHVD
                     LVLDGYRIGLASEESTRDDHGMDPVISGQRAAVILWTEDTPAAYVHIQELGATPVKAP
                     EPWLGRLLIAWAEDPEGHLIQIVQETS"
BASE COUNT          424 a          897 c          812 g          455 t
ORIGIN      
        1 acggcgaccc ggcccgggtc cccatgaccg tcggcgtcct cctgatgatg gtggccggac
       61 tcgccgtcgt gggcatcggc gtgctgatgt acccggtcct caaggaggtc gatccacgcc
      121 tggccggctg gtacccggcc atgcggatca ccgaattcgc ggtctccgcc gcctgcggcg
      181 tctacctact ggccaagttg gaggttgtcc cgaactatct gctgtgggtc tacgtgccca
      241 ccggcgtcgg gggcatcgtc ctcacctacc tgctcttcgt ttctcgactc ctcccgaggg
      301 ccatcgcgct ccttggcctc gtgggatacg gcttgctcac catcggagtg cccctcgacc
      361 tcctcggcat cctcgacatg agcgccggtg ccggcctggt cctcgtcctc cccggcggcc
      421 tgttcgaact ggtgttcctc ccgagctggc tgatcgcgaa agggttcagc gcgccacgct
      481 cctccgagga ctcaggctcg cgagtccagt gacaagcggc agagcgtcct ccgaggccgt
      541 catcgggacc tggtcgacga actgcgcgat ccgcggcacc ttgtaggcgg ccaggtgctc
      601 tcggcagtgg gtcaccagct cctcgctcgt cgcgtgggtg tcggggtggg agccacgcgc
      661 ctcgttgccg tggtagccgc gtcgcccccg gccgacctgg tccgcccgac ggccttttcg
      721 cgacagtcct tgtatggcca gcggggaggc cacaggcgtc tgtcacgccg tgtctgacca
      781 atggaaggac gaatcatggc cgaatcgcaa agtgcgatcc tctcccggac caagagcaag
      841 ggttcgaagc tgagcagtga cacgcgctcg atcctgaccg ccgctctggc gctgcccgtc
      901 ttgacctccc tctcgctcag ttccccgtcg gcagggctca cctcggcaac ggccgcacca
      961 cgcgagcccg cgtctcacgt gcgccatgtg cctgtccccg cccccgtcgt gcacgtgcac
     1021 gtcaccgtga agaaacaccg gatcatcgcg tcgaagagcg gcttccgtcc cggcaacacc
     1081 gttgtcgacc tcagctcaga ggggggcgga atcgccgcca ttcaactgct ccgcctccga
     1141 agcggttaca ccttcgcaga gttccaggcg gatgttgaga gcgacaacat ccaggccttg
     1201 cgtcgcatcg accgcgatgc gatcttctac ggcggcatgc ccgtatacga acggcgagcc
     1261 agccacttcg gcgccaggct cgacgcggga cgctacttcc tcatcgactt cgaccacccg
     1321 cgcatcgggc ggctgctgat tgagggagcc ccggagcgac gctcgctgcc acgaagcaca
     1381 ggatcgatca acatggtgct cgaccaccag caacatcggt tccaaacgcc gcggcggttg
     1441 cctcgttcgg ggtggctgcg gcagacaaac cacacggatg aaccgcactt catggacatg
     1501 atcaggatca agtcctccac gaccaggaag caggtccgca ggttctactc cggccaagga
     1561 cccgaggacc ccagctgggt gctgcacaac tactcgggaa ccttcctcat cagccctggc
     1621 cgcagcgtgg tctggcgcta tgccttcccg cgcggcaagt atctcgaggc ttgctactgg
     1681 cccagccgtg aggacggcac gacgcacgcc gaaatgggca tgtggaactt caccaccctg
     1741 gagtgatgcg ccgtcaacgc ttcggggtgt cggcgtcccc cgccgctcga acggcagatg
     1801 tccgccgtga gtcgctcatc cgcgaccggg tccatccgcc gcgagtgagt gactggggga
     1861 cccggagccg ctgcgctgat ctgccattca ggcgaccgga gcagcgtcac ggacaggtga
     1921 caatctaggt agatggacct gttcaagacc ccgcagatcg tcctgttctg cagggacgtg
     1981 tcgcgtacgg cccagttcta cgagcgcctt ggattcgagg aggcttttcg aactccggcc
     2041 gacgacccgc cgatccatgt cgacctggtc ctcgatggct accgcattgg cctcgcgagc
     2101 gaggagtcca ctcgtgacga tcatggaatg gacccggtga tctccggcca gcgggccgca
     2161 gtgatcctgt ggacagagga cacacccgcg gcgtacgtgc acatccaaga actcggtgca
     2221 acgcccgtga aggcgccgga accgtggctc gggcgactcc tcatcgcctg ggcggaggac
     2281 cccgaagggc acctcatcca gatagtgcag gagacgagct agcccgagtc cacggacccg
     2341 tcaggcgccc acatgtgacg cgatgattgc gtccctccgc cgcggtgcgg gcaggctcgg
     2401 cggccacgga gtcgccgtct ggtcgtgcgg gccattgctt gcaggtctct tcgccgtcga
     2461 tgccctgcga ggatgaggcc tgtgcgccgg tacgagggca ctttctacga cagcaaccgt
     2521 tgggacggct tcgagctccg acccggcgac gtcatcatcg cttcgccgcc caagtgcgga
     2581 acgacgtg
//
LOCUS       DATBFZ010000538         3930 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_4871399, whole genome shotgun sequence.
ACCESSION   DATBFZ010000538 DATBFZ010000000
VERSION     DATBFZ010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 3930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3930
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4871399"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="VH419_16450"
     CDS             <1..360
                     /locus_tag="VH419_16450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7045262.1"
                     /translation="HGHFKCNRHKLTEMPVLWAYARDLFQTPGFGDTTDFVHIKRHYY
                     VVHQDINPTQIIPRGPDLGGWLTPHFREELGGAPFGDGTPPGPPPQSERVPLDHGVGL
                     ERWEEPVEDGDDLRESA"
     gene            complement(363..995)
                     /locus_tag="VH419_16455"
     CDS             complement(363..995)
                     /locus_tag="VH419_16455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7045263.1"
                     /translation="MTDRLARLVRTRLSLHAVAELLLAGPQYERSKSIELRVTPGGFA
                     STRAPDIRVDGADIVAGDRRLALDGRTVDQLAADLGLEPRDLHDVYSDGRDFEASMAL
                     TIDDDAAEEIARAFGIGNAALAKLSTDVRPILWPEHFDVGIALEKVNYGVSPGDDDNA
                     EPYAYVGPWEAPSGERDPFWNKPFGAARAIAELGDEQSVLAFFAEGRDRT"
     gene            complement(1005..1547)
                     /locus_tag="VH419_16460"
     CDS             complement(1005..1547)
                     /locus_tag="VH419_16460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013861350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HEY7045264.1"
                     /translation="MSDDAGVLAAREILTDSFDRIRELVADTCAHVTPAQAAYRLTPT
                     ANSIGWLIWHLTRVQDDHLSGVTGDEQVWTSDGWYDRFGLPLPPHDTGFAHSADQVEL
                     VSADPALLDAYQAAVHEQTIAYVSTIEPDELARIVDEAWDPPVTASVRLVSVIGDCLQ
                     HLGQAAYVRGVLPDDAGGEA"
     gene            1692..2441
                     /locus_tag="VH419_16465"
     CDS             1692..2441
                     /locus_tag="VH419_16465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7045265.1"
                     /translation="MTDAVSDLVMACVAENAAWCALVARTHGIGTERSGGWTRTLRHA
                     PAYHPDLITLDPTVTSEEVAAQLRDRPDCSVKDSFGILDLSGHGFERLFDADWLALPD
                     LTPDVNVKSPKPAGPVRLAPGWHQVSDVDAFRRWVAGLAPDTPLRPALLDDANTRIWL
                     LEEADHVAGFVAHRTQHVVAVTNAFAGGRDAATSWRDLIALANRGWGSASGSLRVVCY
                     EHGDDLVSAHAAGFESHGRLTVWVRAAAAAL"
     gene            complement(2457..2735)
                     /locus_tag="VH419_16470"
     CDS             complement(2457..2735)
                     /locus_tag="VH419_16470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007078376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HEY7045266.1"
                     /translation="MKCPVDETVLQMTERRGVEIDYCPQCRGVWLDRGELDKIIEADL
                     AASTPAPEPARESGRDYYRDEYREDRSYDRGRDRKPKKKSMLGELFDF"
     gene            2781..>3930
                     /locus_tag="VH419_16475"
     CDS             2781..>3930
                     /locus_tag="VH419_16475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7045267.1"
                     /translation="MASHPVRVRLRRLGAASLDEVIGRQTRYMLFATVLVAPLVGVLS
                     HPLPSAAAVPVATNPDIEESCGIDLTVVLDASGSIESSHAVDDVRDAGDALLTALKNT
                     NSTARVTQFATFAEQLAPKTQIDDTSMGNNGALRDALNDYYAPKPPRPSGSNIYSYKG
                     SGSPQSSSSFTQSNSSNQYTNWDQSLDQAAETTPELVLYVTDGDPTAYDFNQAGDPFN
                     DDDVAVNTNRGQADQLTIDRAVQEANRVKNQGSRMLAVGVGSALDNPASADRLTQISG
                     PQVVRNADLANLDSLNDVDVALVTDFKQLAAFMRGVVIQLCSPSLTIQKLAQTADSTA
                     YAPARNWDITVTPTVPGGTFRWLLPNTNQTTSKTVSTDTNGFAQFQWEP"
BASE COUNT          661 a         1366 c         1284 g          619 t
ORIGIN      
        1 cacggtcact tcaagtgcaa ccggcacaag ctcaccgaga tgccggtgct ctgggcgtac
       61 gcgcgtgacc tcttccagac ccccggcttc ggcgacacga cggatttcgt gcacatcaag
      121 aggcactact acgtcgtgca ccaagacatc aacccgaccc agatcattcc ccgcgggccc
      181 gacctgggcg gctggctcac cccgcacttc cgcgaggagc tcgggggcgc accgttcggc
      241 gacgggacac cgcccggtcc gcctcctcag agcgaacgcg tgccgctcga ccacggtgtc
      301 gggctcgagc gctgggagga gccggtggag gacggggacg atctccgcga gtccgcctga
      361 gctcaggtgc ggtcgcgacc ttcggcgaag aacgcgagca cgctctgctc gtccccgagc
      421 tcggcgatcg cacgagctgc tccgaacggc ttgttccaga acggatcgcg ctcgcccgac
      481 ggcgcctccc aaggtccgac gtacgcgtac ggctccgcgt tgtcgtcgtc acctggcgag
      541 acgccgtagt tgaccttctc gagcgcgatc ccgacgtcga agtgctccgg ccacaggatc
      601 ggccgcacat cggtggacag cttcgccagc gcggcgttgc cgatcccgaa ggcccgggcg
      661 atctcctccg ccgcgtcatc gtcgatggtc aatgccatcg acgcctcgaa gtcgcgaccg
      721 tcggagtaca cgtcgtggag atcccgaggc tcgaggccaa ggtcggcagc gagctggtcg
      781 acagttcgcc cgtccagggc aagccgtcga tcgccggcca cgatgtcagc gccgtcgacc
      841 cggatgtccg gggctctggt cgacgcaaaa ccgccgggag tgacgcggag ctcgatcgac
      901 ttgcttcgtt cgtactgcgg tccggccagc agcagctccg ccacggcgtg cagggacagc
      961 cgggtgcgga ccagtctcgc cagccgatcc gtcacggtgc cgcctcaggc ctcgccgccg
     1021 gcgtcgtcag gcaggactcc gcgaacgtag gcagcctggc ccagatgttg caggcagtcg
     1081 ccgatgacgc tcacgagtcg aacgctcgcc gtgaccgggg gatcccacgc ctcgtcgacg
     1141 atccgggcca gctcgtccgg ctcgatcgtg gagacgtagg cgatcgtctg ctcgtggaca
     1201 gccgcctggt aggcgtcgag cagggctggg tcggccgaga cgagctcgac ctggtcggcc
     1261 gagtgggcga agccggtgtc gtggggcggc agcggcagcc cgaagcggtc gtaccagccg
     1321 tcggatgtcc acacctgctc gtcgccggtg acaccggaca gatggtcgtc ctgcactcgg
     1381 gtgaggtgcc agatcagcca tccgatcgag ttcgccgtcg gggtcagccg gtacgccgcc
     1441 tgggcgggag tcacgtgggc acacgtgtcc gcgaccagct cgcggatccg gtcgaacgag
     1501 tccgtcaaga tctcacgggc ggccaagacc ccagcgtcgt cactcatctg cgctcctcct
     1561 ggatcgagtc acaccactca agcacgccag cagaatcccg cgtccccggg cgacgatgac
     1621 atgccctcgc cctgcagcgg tcggtcggcc atgctgggga aggacgatgg cgaacgaggg
     1681 agggatggcc gatgaccgat gcggtctccg acctggtgat ggcatgtgtc gccgagaacg
     1741 cagcgtggtg cgccttggtc gctcgcactc acggcatcgg gaccgagcgg agcggcggct
     1801 ggacccggac actgcgtcat gccccggcgt accaccccga cctgatcacg ctcgatccga
     1861 ccgtgacctc cgaggaggtt gccgcgcagc tgcgtgaccg gccggactgt tcggtgaagg
     1921 acagcttcgg gatcctcgac ctgtccggcc acggtttcga acgactcttc gacgccgatt
     1981 ggctggccct gccagacctg acgcccgacg tcaacgtcaa gtctcccaag ccggcggggc
     2041 cggtcaggct cgcgccgggc tggcaccagg tgagcgatgt cgacgcattc cgtcggtggg
     2101 tcgccggtct cgcgccggac actccgctcc ggccggcgct gctcgatgac gccaacaccc
     2161 gcatctggtt gctcgaggag gccgaccacg tcgcaggatt cgtcgcccac cgcactcagc
     2221 acgtcgttgc ggtgacgaac gcgttcgccg gtggtcgaga cgcggcgact tcctggcgcg
     2281 acctgatcgc gctcgcgaac cgtggttggg gttcggcgtc aggatcgttg cgcgtggtct
     2341 gctacgagca cggcgacgac ctcgtgagtg cacatgcggc ggggttcgag tcccacggcc
     2401 gcctgaccgt ctgggtgcgc gcagctgcgg cggcgctgtg acgtcctggc tcccggtcag
     2461 aagtcgaaca gctcgccgag catgctcttc ttcttgggct tgcggtcgcg gccgcgatcg
     2521 tagctgcgat cttcccggta ctcgtcacgg taatagtcgc gtccggactc acgggccggc
     2581 tccggcgctg gagtgctcgc ggccaggtcg gcctcgatga tcttgtcgag ctctccgcgg
     2641 tcgagccaga cgcctcgaca ctgcgggcag tagtcgatct cgacaccacg gcgctcggtc
     2701 atctgaagga cggtctcgtc aacagggcac ttcattgcgc cacgttacct ggaacttccg
     2761 gtatgccgga ctgtcgaatc gtggcatctc acccggtgcg ggtgaggttg cgccggttgg
     2821 gcgctgcgag cctcgatgag gtgatcggac gccagacccg gtacatgctc ttcgcaaccg
     2881 tcctggttgc cccgcttgtg ggcgtcctta gccatccgct gccctccgca gcagccgtgc
     2941 cggtggccac caacccagac atcgaggagt cgtgtggcat cgacctgacc gtggtgttgg
     3001 acgcctccgg ctcgatcgag tcgtcgcacg ccgtggacga cgtccgcgat gcgggcgacg
     3061 ccttgttgac ggcgttgaag aacaccaact cgacggcgcg cgtcacgcag ttcgcgacgt
     3121 tcgccgagca gctggcgccg aagacccaga tcgacgacac cagcatggga aacaacggcg
     3181 ccctgcgtga cgcgctcaac gactactacg ccccgaagcc tccccgaccc agcggatcga
     3241 acatctactc ctacaagggc agcgggagcc cccagagcag tagcagcttt acgcagagca
     3301 acagctccaa ccagtacacg aactgggacc agtcactcga ccaggccgct gagacaacgc
     3361 ccgagctggt tctgtacgtc accgacggtg acccgacagc ctacgacttc aaccaggccg
     3421 gagatccgtt caatgacgac gacgtcgccg tcaacaccaa cagaggccaa gccgaccagc
     3481 tcaccatcga tcgggccgtc caggaagcca accgggtcaa gaaccagggc agccggatgc
     3541 tcgctgtcgg ggtcggctcg gctctcgaca acccagccag cgcggaccgg ctcacccaga
     3601 tctccggtcc ccaggtcgtc cggaacgccg acctcgcgaa cctcgacagc ctgaacgacg
     3661 tcgacgtcgc gctggtcacc gacttcaagc agctggccgc cttcatgcgc ggggtcgtga
     3721 tccagctgtg ctccccgtcg ctcacgatcc agaagctcgc gcagactgcg gacagcacgg
     3781 cgtacgcgcc ggcgcggaac tgggacatca ccgtcacccc gacggtgccc ggcggcacgt
     3841 tcagatggtt gctgccgaac acgaaccaga cgacgtcgaa gaccgtcagc acggacacca
     3901 acgggttcgc gcagttccag tgggaaccca
//
LOCUS       DATBFZ010000539         7432 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_5197818, whole genome shotgun sequence.
ACCESSION   DATBFZ010000539 DATBFZ010000000
VERSION     DATBFZ010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 7432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7432
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5197818"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="VH419_16480"
     CDS             <1..278
                     /locus_tag="VH419_16480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017936375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HEY7045268.1"
                     /translation="VITIEDHCQVGPNVQFLTPVHPVEPRRREDKWETARPITVGRNV
                     WIGGGAIICPGVTIGADTVVGAGAVVTKDLPAGVVAQGNPARVVRDV"
     gene            379..1587
                     /locus_tag="VH419_16485"
     CDS             379..1587
                     /locus_tag="VH419_16485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012924002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HEY7045269.1"
                     /translation="MADEQSQVVGVGAGAAGFSGYERGGTGVASELATEDMVLNIGPQ
                     HPATHGVLRLRITLDGERIVECEPIIGYMHRGVEKLFEVRDYRQIIVLANRHDWLSAF
                     SSELGVVLAVERMLGMEVPERATWTRTLLAELNRVLNHLMFLGSYPLEVGAITPIFYA
                     FRERETIQEVMEEISGGRMHYMFNRVGGLKEDLPAGWLGRARAAVAAVRSRLTQIEDL
                     IFGNEIFRARTVGVGVLRPETIAQYGVSGPIARAAGVDFDLRRDDPYLAYDELTTSGV
                     FRVCTRSDGDCYARYGMLLDQIHVSLDVADACLDTIASLPQGPINVRLPKILKAPEGH
                     TYAWTENPLGINGYYLVSRGEKTPWRLKLRSASFNNISVLPTMLPGCVVADMIAILGS
                     MFFVVGDIDK"
     gene            1607..2119
                     /locus_tag="VH419_16490"
     CDS             1607..2119
                     /locus_tag="VH419_16490"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEY7045270.1"
                     /translation="MPHQPPSNVKHDAHGRWHEFGGKILRYTTGSVVAAICSEVTFVV
                     VYGGLDASPAWATCIGWLAGAIPNFWLNRSWTWRVRGRPHLLREVLPYVVIIVVTLVT
                     AVVVTAAVDNWLKGKDLGPGVQTFLVSLAFLGVYGAMFLLRFFLLDKLFGRAHLKGAD
                     AATELTPVRR"
     gene            2116..2871
                     /locus_tag="VH419_16495"
     CDS             2116..2871
                     /locus_tag="VH419_16495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020386805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY7045271.1"
                     /translation="MSADDEGSRGDEGAGAFTLATGRGMEDGSGANQRRYRAYQFELI
                     SEHCGPSVLEVGAGLGEFASQFSGLERHIVTDVDPDAVASMAARFADRPEVEARQFDV
                     SSGPLDIGAAVSTIVAINVLEHIEPDCETLRALATMVRPGGSIVLWVPGYQQLYGDFD
                     RKVGHVRRYTPTTLRAAIVGAGLAPGVVKPVNLLGGVAWWLTVRRGGVGSPRPGLLQT
                     YDRLVVPATRLLERRVTPPFGQSVLGVARVPAA"
     gene            complement(2816..4855)
                     /locus_tag="VH419_16500"
     CDS             complement(2816..4855)
                     /locus_tag="VH419_16500"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY7045272.1"
                     /translation="MHPTDSPDSEVMVPEAPWLRRHGWFLVVLAVGAVLRVCFVVGFR
                     PAFQIGASFPYLSASSGPRPSPPPAFGYSWLELAPLSRLTDSLTLVVLVQHVIGLLVG
                     IAVYVLLQRWGVWRWLSVVAAAPALLDSHLLTSEHLVLPDTLFVALCTGATLVLGWRS
                     RPRLVTAASAGVLLGTASTVLAVGPPLLALGVLFWLLVGISWPHRVAGAVLLVLGFCV
                     PIALYAGWQDSPTVDEVKATIGWGAGSGDVTDSVTGVVNGSDFDADRWDPDSFAWMAA
                     VTDGADVGQDGLTRHDPAGDVVVGYSGWGTIPLGWLFAGTAVALIAALGLYRARRSGM
                     RAVCALPAALPVAVFTGAVIGDEASWHSLPLAIVLVPAAGALAVTAILRGTRSPAADR
                     PQVDLVDEAAVEDFAERYGEPAFAPIVVVIAAFNESTGLPRVLDTLATSVCGLDVDVA
                     VVDDGSTDETAAVVRGHPRAYLASCRTNRGQGAALRLGYRLARTHGARFIITTDADGQ
                     YDAADFPAVLGPVVAGDADLVTGSRRLGHQHTYDRLRRLGVHVFGWLVSCLVGQYHTD
                     TSFGLRAMRAELTEDVTLNQPQYQSAELLIGAHSHGWRVLEVPATMHVRAAGSTKKGR
                     NLVYGTRYARVAVGTWWREGCPRPVSDEAPALQPLRVVRRRARVPRPAQTDRTAV"
     gene            5131..7089
                     /locus_tag="VH419_16505"
     CDS             5131..7089
                     /locus_tag="VH419_16505"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HEY7045273.1"
                     /translation="MDYRSALSDTLTVLRSVATEYQPAVLANAFGPESMVMTDLIAKH
                     RLDIEIFTIDTGRLPEETHALAHSVHQRYGPIIKVVNPDADEVTAWTSRHGLNGFYDS
                     VDLRHGCCHVRKVAPLRRVLAGKRAWVTGLRRQQSVTRRDLTVSSWDDDYGLQKFNPM
                     VGWTSEQVWAYIRDHEVPYNKLYDRGFTSIGCAPCTRAVSKGEDVRAGRWWWELDSVK
                     ECGVNFDPTTGRMQRTPTVAEPQTAASTDHEGKAMRVLILGGDGFCGWPTALHLSALG
                     HEVAIVDNLSRRKIDIELEVSSLTPIKPMGERLRTWKEITGNDIAFHDIDVAVNYQRL
                     LDLFEGWKPQALVHFAEQRAAPYSMKSSRHKRYTVDNNLNATNNVLAAVVESGLDIHV
                     VHLGTMGVYGYGTAGMKIPEGYLQVKVETGQGTLVDQEILYPANPGSIYHLTKTQDQL
                     LFAYYNKNDHVRVTDLHQGIVWGTQTKETKLDERLVNRFDYDGDYGTVLNRFLMQAAI
                     GYPLTVHGTGGQTRAFIHIQDTVRCIQIALENAPERGDRVHIFNQMTETHRVRDLAKM
                     ISDRTGVKIAKLPNPRNEADENDLHVANDRFLHLGLTPITLSEGLMEEVTEITKKYAD
                     RCDQTKIPCVSVWSADSAAAQARVTDSI"
     gene            complement(7102..>7432)
                     /locus_tag="VH419_16510"
     CDS             complement(7102..>7432)
                     /locus_tag="VH419_16510"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEY7045274.1"
                     /translation="VYGDGSQTRDYLYVGDLVEAIWAAVESDARGAYQLGSGRPTTVN
                     QLLDAIRTVTRRDLDVVYENFRPGEIRDTWCDIGKARASLGFDPMTKLEDGLRYTWEW
                     FSAQRQD"
BASE COUNT         1330 a         2505 c         2473 g         1124 t
ORIGIN      
        1 ccgtcatcac gatcgaggac cactgtcagg tcggcccgaa cgtgcagttc ctcactcctg
       61 tgcaccccgt ggagccgcga cgtcgcgagg acaagtggga gacggcccgg ccgatcacgg
      121 tcggacgcaa cgtctggatc ggcggtggtg cgatcatctg ccccggcgtc acgatcggcg
      181 cggacacggt cgtcggcgcg ggcgctgtcg tgaccaagga cctccccgca ggtgtggttg
      241 cccaaggcaa ccctgcccgg gtcgtgcgag acgtctgacg aaggcgcccc aaccagaact
      301 cacggttcat gaggccacaa ctcacggttc gacgaaccac aactcacggt tcaacgagag
      361 gcgtgggagc atgacggcgt ggcggatgag cagagccagg tcgtcggtgt cggcgcaggt
      421 gctgccggct tctccgggta cgaacgcggt ggaaccggcg ttgcgagtga gctggccacc
      481 gaggacatgg tcctcaacat cggtccgcag cacccggcga cccacggcgt actccggctg
      541 cgtatcacgc tggacggcga gcgcatcgtc gagtgcgagc ccatcatcgg ctacatgcac
      601 cggggggtcg agaagctctt cgaggtccgc gactaccggc agatcatcgt cctcgccaac
      661 cggcacgatt ggctgtcggc cttctcctcc gagctcggcg tcgtactggc cgtcgagcgg
      721 atgctcggca tggaggtgcc cgagcgggcg acctggaccc ggacgctgct cgccgagctg
      781 aaccgcgtcc tgaaccatct gatgttcctc ggctcatacc cgctggaggt cggcgcgatc
      841 actccgatct tctatgcctt ccgggagcgg gagacgatcc aggaggtcat ggaggagatc
      901 tccgggggcc gcatgcacta catgttcaac cgggtcggcg ggctgaaaga ggacctgccg
      961 gccggctggc tgggtcgcgc gcgtgcagcg gtcgctgcgg tccggtcccg gctgacccag
     1021 atcgaggacc tcatcttcgg caacgagatc ttccgcgccc ggaccgtggg tgtcggcgtc
     1081 ctacgtccgg agacgatcgc gcagtacggc gtcagcggcc cgatcgcgcg cgcagccggt
     1141 gtcgacttcg acctgcgcag ggacgacccc tacctggcgt acgacgagct gaccacatcc
     1201 ggcgtgttcc gggtctgcac gaggagtgac ggggactgtt atgcgcgcta cggcatgctg
     1261 ctggaccaga tccacgtctc actcgacgtc gccgatgcct gtctggacac gatcgcatcg
     1321 ttgccgcagg gcccgatcaa cgtccggctg cccaagatct tgaaggctcc cgagggacac
     1381 acgtacgcgt ggacggagaa ccccctcgga atcaacggct actaccttgt ctcgcgcggt
     1441 gagaaaaccc cgtggcggct caagctgcgc tcggcgtcct tcaacaacat ctccgtcctc
     1501 ccgacgatgc tgcccggttg tgtcgtggcc gacatgatcg ccatcctcgg ctcgatgttc
     1561 ttcgtcgtcg gcgacatcga caagtgacga ccgtaggatc gacgccatgc ctcaccagcc
     1621 gccgtcgaac gtcaaacacg acgcgcacgg ccggtggcac gagttcggcg ggaagatcct
     1681 ccggtacacg acgggctcgg tggtggcggc gatctgcagc gaggtcacct tcgtcgtcgt
     1741 gtacggcggc ctggatgcgt cgccggcatg ggcgacgtgt atcggctggc tggcgggagc
     1801 gattcccaac ttctggctga accggagctg gacgtggcgg gttcgggggc ggccgcacct
     1861 gttgcgcgag gtgctcccct acgtcgtgat catcgtggtc acgctggtca ctgccgtggt
     1921 ggtcaccgca gcggtggaca actggttgaa gggcaaggat ctcggtcccg gtgtccagac
     1981 gttcctcgtg agcctggcgt tcctcggcgt gtacggcgcg atgttcctgc tccgcttctt
     2041 cctgctcgac aagctgttcg gccgcgctca tctcaagggt gcagacgcgg ccaccgagct
     2101 cacgccggta cggcgatgag cgccgacgac gagggcagcc gtggcgatga aggagccggc
     2161 gcgttcacgc tggcgaccgg acgcggcatg gaggacggct ctggcgccaa ccagcggcgc
     2221 taccgggcgt accagttcga gctgatatcc gagcactgcg gcccttcggt cctcgaggtc
     2281 ggcgcggggc tcggcgagtt cgcgagccag ttcagcgggc tcgagcggca catcgtcacc
     2341 gacgtcgacc ccgacgcggt cgcctcgatg gcggcccgtt ttgcggatcg acccgaggtc
     2401 gaggctcggc agttcgatgt gtcgtctggc ccgctcgaca tcggtgcggc ggtgtccacg
     2461 atcgtggcga tcaacgtgct cgagcacatc gagccggact gcgagacact gcgggcactg
     2521 gcgacgatgg tgaggccagg cggttcgatc gtgctgtggg tgccgggcta tcagcagctc
     2581 tacggcgact tcgaccgcaa ggtcggtcac gtacgtcgct acacgccgac gaccttgcgc
     2641 gccgcgatcg tcggcgccgg ccttgctccg ggtgtcgtga aaccggtcaa cctgctcggg
     2701 ggcgtggcct ggtggctcac cgtacgccgc ggcggcgtcg gctcgccgag gcccggcctt
     2761 ctccagacgt acgacaggct ggtggtgccg gcgacgagac tgctcgagcg cagggtcaca
     2821 ccgccgttcg gtcagtctgt gctgggcgtg gcacgcgtgc ccgccgcctg acgacgcgga
     2881 gcggctgcaa cgccggcgct tcgtcggaca ccggacgcgg gcatccttcc cgccaccacg
     2941 ttccgaccgc gacacgcgca taacgcgttc cgtacacgag gttgcggccc ttcttggtcg
     3001 agccggctgc ccggacgtgc atcgtggctg gtacctccag gacccgccag ccgtgcgagt
     3061 gagctccgat gagcagctcc gccgactggt actgcggctg gttcaaggtg acgtcttcgg
     3121 tcagctctgc gcgcatcgcc cgcagtccga acgacgtgtc ggtgtggtac tgcccgacca
     3181 ggcacgacac gagccagccg aagacatgga cgccgagacg acgcagccgg tcatacgtgt
     3241 gctgatgtcc gaggcgacgt gagccggtga ccagatccgc gtccccagcg accaccggac
     3301 ccagcacggc agggaagtct gctgcgtcgt actgcccgtc ggcgtcggtg gtgatgatga
     3361 atcgggctcc gtgcgtccgg gcgagccggt agccaagccg aagcgctgcg ccttgaccgc
     3421 ggtttgtccg gcacgaggcg aggtaggccc gcggatggcc gcgcacgacc gctgcggtct
     3481 cgtcggtgct gccgtcgtcg acaaccgcga cgtccacgtc caacccacac accgacgtcg
     3541 cgagggtgtc cagcaccctg ggcagaccgg tgctctcgtt gaacgccgcg atcacgacga
     3601 cgatgggggc gaatgccggt tcgccgtacc gctcggcaaa atcctccacc gccgcttcgt
     3661 cgacgaggtc gacctgtgga cgatcagcag ccggggaccg ggtcccccgc aggatcgccg
     3721 tgacggccaa cgcgccggct gcggggacca acacgatcgc gagcggcagg ctgtgccagg
     3781 acgcctcatc gccgatgacc gcgccggtga acaccgctac ggggagagcg gcgggaagtg
     3841 cacacaccgc ccgcatgccg gatcgacgag cccggtagag accgagcgcc gcgatcagcg
     3901 caacagccgt gcccgcgaaa agccatccga gcggaatcgt cccccatcct gagtatccga
     3961 cgaccacgtc accggccggg tcatgccgcg tcaggccgtc ctgaccgaca tccgcgccat
     4021 cggtcacggc ggccatccat gcgaacgagt ccgggtccca acggtcagcg tcgaagtcgg
     4081 acccgttgac gacaccggtc acggagtccg tgacgtctcc agaccccgcg ccccatccga
     4141 tcgtcgcctt gacctcgtcg acggtcggcg agtcctgcca gccggcgtac aaggcgatcg
     4201 ggacgcagaa gccgagcacg agcagcacgg ctccagccac tcgatgcggc caggagatac
     4261 cgacgagcag ccagaacagg acgccgagag ccagcaacgg cggaccaacg gcgaggacgg
     4321 tggatgcggt cccgagcagt acaccggcgc tcgcggcggt gacgagtcgc ggccgcgaac
     4381 gccagccgag aacgagcgtc gcgccggtgc agagtgcgac gaagagggtg tcggggagca
     4441 ccaggtgctc gctcgtcagc agatgcgagt ccaacaacgc tggtgcagcc gcgaccacgg
     4501 acagccatcg ccacacaccc cagcgctgca acagcacgta gaccgcgatc ccgacgagca
     4561 agccgatcac gtgctggacg aggaccacca gcgtgagcga atcggtcaga cgcgacagcg
     4621 gagccagctc cagccaggag taaccgaagg caggcggcgg gctgggccgg ggcccggagc
     4681 tcgcagagag atatgggaag gacgcaccga tctggaaggc cggacggaag cccacgacga
     4741 agcacacccg cagcactgct ccgacggcga gcacgacaag gaaccatccg tgccgccgca
     4801 gccacggcgc ctccggcacc atcacctcgg agtcggggct gtccgtcgga tgcataacag
     4861 gaccctagac ggtggcgggc gcccccgcct gcgcgcacgc ccggtggccg tgtacaccga
     4921 cgcgagcatc ccggcggccg cgtctcgccg actacgcaga aacggggcaa cgggtcagcc
     4981 aacgggaccg cgaccgggat tcgggcgcgg attcggcccg cctcggtgtt agcatcgcgg
     5041 gcacctgatc gtcgcgcatg gcaccacatc aaggcaccac atcaaggcac cacgtcaagg
     5101 caccacgtca aggcataggg gagttcacac gtggactacc gttctgccct ctccgacacg
     5161 ttgaccgtgc tgcgcagcgt tgccaccgaa taccagcccg cggtgttggc gaacgcgttc
     5221 ggccccgaga gcatggtcat gaccgacctg atcgccaagc accggctcga catcgagatc
     5281 ttcaccatcg acaccggacg gctcccggag gagacacatg cgctcgcgca cagcgtccac
     5341 cagcgctacg ggcccatcat caaggtcgtc aaccccgacg ccgacgaggt cacggcctgg
     5401 acgtctcggc acggcctcaa cggcttctac gactccgtcg acctgcggca cggctgttgc
     5461 catgtccgta aggtcgcgcc gttgcgtcgc gtccttgccg ggaaacgtgc ctgggtcacc
     5521 gggctgcgcc ggcagcagtc ggtgacccgg cgcgacttga cggtcagttc ttgggacgac
     5581 gactacggac tccagaagtt caaccccatg gtgggctgga ccagcgagca ggtatgggcg
     5641 tacatccgcg accacgaggt gccctacaac aagctgtacg accgaggctt caccagcatc
     5701 ggttgtgcgc cgtgcacgag agcggtgtcg aagggcgagg acgttcgagc gggtcgctgg
     5761 tggtgggagc tggactccgt caaggagtgc ggagtgaact tcgaccccac gaccggcagg
     5821 atgcaaagga cccccactgt tgcagagccg cagaccgcgg cctcgacgga ccacgaaggg
     5881 aaagcgatgc gagtactgat tctgggcggg gacggcttct gcggctggcc cacggctctc
     5941 cacctttccg cactgggtca cgaggtcgcg atcgtcgaca acctgtcccg ccgcaagatc
     6001 gacatcgagc tggaggtctc ctccctgacg ccgatcaagc cgatgggtga acggcttcgc
     6061 acgtggaagg agatcaccgg caacgacatc gccttccacg acatcgatgt tgcagtgaac
     6121 taccagcgac tgctcgacct gttcgagggc tggaagccgc aggctctggt gcacttcgcg
     6181 gagcagcggg cggcgccgta ctccatgaag agcagccggc acaagcgcta caccgtcgac
     6241 aacaacctca acgccacaaa caacgtcctg gcggcggtcg tggagagtgg gttggacatc
     6301 catgtcgtgc acctcggcac gatgggggtc tacggctatg gaacggccgg gatgaagatc
     6361 cccgagggtt acctgcaggt caaggtcgag accgggcagg gcacgctcgt cgaccaggag
     6421 atcttgtacc cggcgaaccc cggcagcatc taccacctga ccaagacgca ggaccagttg
     6481 ctgttcgcct actacaacaa gaacgaccac gttcgggtga ccgacctgca ccagggcatc
     6541 gtgtggggca cccagacgaa ggagacgaag ctcgacgagc ggctggtcaa ccggttcgac
     6601 tacgacggcg actacggcac cgtcctcaac cgcttcctga tgcaggcggc gatcggctac
     6661 ccgctgaccg tccacggcac aggcggacag acgcgcgcct tcatccacat ccaggacacg
     6721 gtcaggtgca tccagatcgc gctggagaac gcgcccgagc gtggtgaccg ggtccacatc
     6781 ttcaaccaga tgaccgagac ccatcgggtc cgtgacctgg ccaagatgat ctccgaccgg
     6841 accggcgtga agattgcgaa gctgccgaac ccgcgcaacg aagccgacga gaacgacctc
     6901 cacgtcgcca acgaccgctt cctgcacctc ggactcacgc ccatcaccct cagcgagggc
     6961 ttgatggagg aagtcaccga gatcaccaag aagtacgccg atcgctgcga ccagacgaag
     7021 atcccctgcg tctcggtgtg gagcgccgac tcggccgccg cccaggcgag ggtcaccgat
     7081 tcgatctgag cgccgccccg ttcagtcctg ccgctgggcg ctgaaccact cccaggtgta
     7141 ccgcaggccg tcctcgagct tcgtcatcgg atcgaacccg aggctcgcgc gggccttccc
     7201 gatgtcacac caggtgtcac ggatctcgcc gggacgaaag ttctcgtaga cgacgtcgag
     7261 gtcgcgtctc gtcaccgtgc ggatggcgtc gagcagctgg ttgacggtgg tcggcctccc
     7321 gctgccaagc tggtaggcgc ctcgggcatc gctctcgacg gccgcccaga tggcctcgac
     7381 gagatcgccg acgtacaagt agtcgcgggt ctgactgccg tcgccgtaca cg
//
LOCUS       DATBFZ010000540         1541 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_6186123, whole genome shotgun sequence.
ACCESSION   DATBFZ010000540 DATBFZ010000000
VERSION     DATBFZ010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6186123"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..690)
                     /locus_tag="VH419_16515"
     CDS             complement(<1..690)
                     /locus_tag="VH419_16515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020392570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3050 domain-containing protein"
                     /protein_id="HEY7045275.1"
                     /translation="MSRYDWGKTHPGIERLEKNITEARDRVVKHPLYANLDTHEALVT
                     FMEHHIFAVWDFMSLLKSLQRNLTCVTVPWIPTGPTGSRRLINDIVMVEESDELGDGF
                     ISHFELYVNGMREAGADTTAIDSLISLLREERPVLEALREAGVPKASIMFAGVTWSII
                     ESSPVHCQAAAFAFGREDLIPDMFTQVVAINERSKKLGTFVDYLERHIEVDGEQHTPM
                     AMQMVTDLCGDD"
     gene            complement(726..>1541)
                     /locus_tag="VH419_16520"
     CDS             complement(726..>1541)
                     /locus_tag="VH419_16520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020392569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEY7045276.1"
                     /translation="YLTKKLFTDDEVDELVEASERYYAGERARRLPSRPPRLAYWEPG
                     SGNVQRHNDYVHYESDALAKILCKPLLGAVAARLAQADEIRVFQSTLIYKPPVAGEPS
                     NIVPWHFDKHYWSSSSSEKMLTAFIPFHDCDEEMGTITMVDGSNRWEEIGAEDSQTRH
                     FAERDRSELEDMLAANAAYNGVEVTKVPMVIPKGHMSFHHCRTYHGSGANVSSRPRRA
                     VSLHLQDGANAFREFPLSDGTHAFYNHDSLVRHTADGRPDYSDPDYCPTLWRD"
BASE COUNT          215 a          507 c          532 g          287 t
ORIGIN      
        1 gtcgtcgccg cacaggtcgg tgaccatctg catggccatc ggggtgtgct gctcgccgtc
       61 gacctcgatg tgccgctcga ggtagtcgac gaacgtgccg agcttcttgc tgcgctcgtt
      121 gatcgcgacc acctgggtga acatgtcggg gatcaggtcc tcgcggccga acgcgaacgc
      181 ggccgcctgg cagtgcaccg gcgagctctc gatgatgctc caggtcacgc cggcgaacat
      241 gatcgacgcc ttgggcacgc ccgcctcgcg cagcgcctcc agcaccgggc gctcctcacg
      301 cagcagcgag atgagcgagt cgatcgcggt ggtgtccgcg cccgcctcgc gcatgccgtt
      361 gacgtagagc tcgaagtggg agatgaaccc gtcgcccagc tcgtcggact cctcgaccat
      421 cacgatgtcg ttgatgagcc tgcggctgcc cgtcgggccc gtggggatcc acggcacggt
      481 cacgcaggtc aggttgcgct gcaacgactt cagcagcgac atgaagtccc acaccgcgaa
      541 gatgtggtgc tccatgaacg tcacgagcgc ctcgtgggtg tcgaggttcg cgtacagcgg
      601 gtgcttgacc acccggtcgc gggcctcggt gatgttcttc tcgagacgct cgatgccggg
      661 atgggtcttg ccccagtcgt atctggacac gctggttctc cttgtcgtga gggggttcgg
      721 cgggatcagt cgcgccagag ggtgggacag tagtcgggat cggagtagtc gggtcgcccg
      781 tcggccgtgt gccgcaccag cgagtcgtgg ttgtagaagg cgtgggtgcc gtccgagagc
      841 gggaactcgc ggaaggcgtt cgcgccgtcc tgcaggtgca gcgacaccgc gcggcggggc
      901 ctgctgctga cgttcgcgcc gctgccgtgg taggtccggc agtggtggaa gctcatgtgt
      961 cccttgggga tcaccatcgg gaccttggtg acctcgacgc cgttgtacgc cgcgttggcc
     1021 gcgagcatgt cctccagctc gctgcggtcc cgctcggcga agtggcgcgt ctggctgtcc
     1081 tcggcgccga tctcctccca gcggttgctg ccgtcgacca tcgtgatcgt gcccatctcc
     1141 tcgtcacagt cgtggaaggg gatgaacgcg gtcagcatct tctccgagga cgacgaggac
     1201 cagtagtgct tgtcgaagtg ccacggcacg atgttcgacg gctcgcccgc caccggcggc
     1261 ttgtagatca gcgtcgactg gaacacccgg atctcgtccg cctgcgcgag ccgcgcggcc
     1321 accgcgccga gcagcggctt gcacaggatc ttggccagcg cgtcgctctc gtagtgcacg
     1381 tagtcgttgt gccgctggac gttcccgctg cccggctccc agtaggccag ccgcggcggg
     1441 cggctcggca gccgcctggc ccgctccccg gcgtagtacc gctcgctcgc ctcgaccagc
     1501 tcgtcgacct cgtcgtcggt gaacagcttc ttggtgaggt a
//
LOCUS       DATBFZ010000541         2441 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_6188494, whole genome shotgun sequence.
ACCESSION   DATBFZ010000541 DATBFZ010000000
VERSION     DATBFZ010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6188494"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1195
                     /locus_tag="VH419_16525"
     CDS             <1..1195
                     /locus_tag="VH419_16525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEY7045277.1"
                     /translation="VRRESEAKIRFELEQTFMFYLPRICEHCLNPSCMASCPSGAIYK
                     RSEDGIVLVDQDRCRGWRQCITGCPYKKIYFNHKSGKAEKCTFCYPRVEVGIPTVCSE
                     TCVGRLRYLGLFLYDADAVTAAASVPDEKDLHQAQLDLLLDPNDPAVVAAAREQDIPE
                     DWLDAARRSPVYALAKTYGVALPLHPEYRTMPMVWYVPPLSPVVDLLAEQGHDAEDRD
                     VLFGAIDSLRIPVEYLAELFTAGDTAVVTGVLHKLAAMRAYMRGVNLGREPDASIPAS
                     VGMTEESIYAMYRLMAIAKYDERYVIPKAHVEQAHELEEIGCSLDYDEGPGMYGSAAF
                     GEASGRPVPVAVETYHALRRRQSSDVPAGDAQLRGRVNLLNWDGNGTPEGLFPPSRHD
                     EEASR"
     gene            1192..1860
                     /gene="narJ"
                     /locus_tag="VH419_16530"
     CDS             1192..1860
                     /gene="narJ"
                     /locus_tag="VH419_16530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HEY7045278.1"
                     /translation="MRRGLDKLDRRRDVDRRRGARVVHQVASWCLCYPDEALVEMIPM
                     LRTALAEESVVSFDPFLEHVERTPVAQLEREYVDVFDLSRRHALYLSYWTDGDTRRRG
                     EVLAGFKQRYRRSGFLVDTRGELPDFLPLVLEYAAVADPVDGTALLEEYRPSLELLRI
                     GLAEDGSPYADVVGAVCATLPGVSPPDRAAVMAMAAAGPPTETVGLEPYDPRLLPLAE
                     EARR"
     gene            1857..>2441
                     /gene="narI"
                     /locus_tag="VH419_16535"
     CDS             1857..>2441
                     /gene="narI"
                     /locus_tag="VH419_16535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HEY7045279.1"
                     /translation="MNVVLWGVLPYLVIATLVGGTIWRYRYDKFGWTTRSSQLYESRL
                     LRIGSPLFHFGILVVLVGHVVGLLIPESWTDAVGVSEHAYHLQALTLGGIAGFCTLVG
                     IIILVVRRRTTGPVFMATTNNDKAMYVVLVGAIVLGLLTTLVAIGAGADAHNYRETVS
                     PWFRSLVRLNPDVDAMTAAPAQFKVHALVGMALFA"
BASE COUNT          355 a          886 c          814 g          386 t
ORIGIN      
        1 ggttcgtcgg gagtccgagg ccaagatcag gttcgagctc gagcagacct tcatgttcta
       61 cctgccccgg atctgcgagc actgcctgaa tcccagctgc atggcctcct gcccgtcggg
      121 ggcgatctac aagcgcagcg aggacggcat cgtgctggtc gaccaggacc gctgtcgcgg
      181 ctggcgccag tgcatcaccg gatgtccgta caagaagatc tacttcaacc acaagtccgg
      241 caaggccgag aagtgcacgt tctgctaccc ccgcgtggag gtcggcatcc cgacggtgtg
      301 ctcggagacc tgcgtgggcc ggctgcgcta cctcggcctc ttcctgtacg acgccgacgc
      361 ggtcaccgcg gccgcctcgg tccccgacga gaaggacctc caccaggcac agctcgacct
      421 gctgctcgac ccgaacgacc ccgccgtggt cgccgcggcc cgggagcagg acatcccgga
      481 ggactggctg gacgcagcac gtcgttcccc cgtctacgcc ctggccaaga cctacggcgt
      541 cgccctgccg ctgcacccgg agtaccgcac gatgccgatg gtctggtacg tgccaccgct
      601 gtcaccggtg gtcgacctgc tcgcggagca gggacacgac gccgaggacc gcgacgtgct
      661 cttcggagcg atcgactccc tgcggatccc ggtcgagtac ctcgccgagc tcttcaccgc
      721 cggcgacacc gcggtggtga ccggggtcct gcacaagctg gccgcgatgc gggcctacat
      781 gcgcggggtc aacctcggtc gtgagcccga cgcctcgatc cccgcctcgg tcggaatgac
      841 cgaggagagc atctacgcga tgtaccggct gatggcgatc gccaagtacg acgagcgcta
      901 cgtcatcccc aaggcgcacg tcgagcaggc gcacgagctc gaggagatcg gctgctcgct
      961 cgactacgac gaagggcccg gcatgtacgg ctcggcggcg ttcggcgaag ccagcggccg
     1021 gcccgtgccg gtcgccgtgg agacctacca cgccctgcga cggcgccaga gcagcgacgt
     1081 ccccgccggc gacgcgcagc tgcgcggccg ggtcaacctg ctcaactggg atggcaacgg
     1141 cacccctgag gggctgttcc ctccgtcgcg gcacgacgag gaggcctcgc gatgaggcgt
     1201 ggtctcgaca agctcgaccg acgccgcgac gtcgaccgac gccggggcgc gcgggtggtg
     1261 caccaggtcg cctcgtggtg cctgtgctac cccgacgagg ccctcgtgga gatgatcccg
     1321 atgctgcgaa cggcgctggc cgaggagtcc gtcgtgtcgt tcgacccctt cctcgagcac
     1381 gtggaacgga ctccggtcgc ccagctcgag cgggagtacg tcgacgtctt cgacctgtca
     1441 cgtcgccacg cgctctacct ctcctactgg accgacggcg acacccgacg ccgcggtgag
     1501 gtgctcgccg ggttcaagca gcgctaccgg cgcagcggct tcctggtcga cacccgcggt
     1561 gagctccccg acttcctgcc tctggtcctc gagtacgccg cggtcgccga ccccgtggac
     1621 gggacggctc tcctcgagga gtaccggccc agcctggagc tgctccggat cggcctggcc
     1681 gaggacggct cgccgtacgc cgacgtggtc ggagccgtgt gcgcgacgct ccccggcgtc
     1741 tcacccccgg accgcgccgc cgtgatggcg atggctgcgg ccggaccgcc caccgagacg
     1801 gtcgggctgg agccctacga cccgcgcctg ctgccgctcg cggaggaggc gcgtcgatga
     1861 acgtcgtgct ctggggcgtg ctgccctacc tggtcatcgc cacgctcgtc gggggcacga
     1921 tctggcggta ccgctacgac aagttcggtt ggacgacccg ctcgtcccag ctctacgagt
     1981 cccggctcct gcggatcggc tcgccgctgt tccacttcgg gatcctcgtg gtcctggtcg
     2041 ggcacgtggt cggcctgctc atcccggaga gctggaccga cgcggtgggc gtctccgagc
     2101 acgcctacca cctgcaagca ctcacgctgg gtgggatcgc cggcttctgc accctggtcg
     2161 ggatcatcat cctggtcgtg cgtcgacgta cgacgggccc ggtgttcatg gcgaccacca
     2221 acaacgacaa ggccatgtac gtcgtcctcg tcggggcgat cgtcctcggc cttctgacca
     2281 cgctggtcgc gatcggcgcg ggcgcggacg cccacaacta ccgggagacc gtctcgccct
     2341 ggttccgctc cctggtccgg ctcaacccgg acgtcgacgc gatgacggcc gctcctgcgc
     2401 agttcaaggt ccatgccctg gtcgggatgg cgctcttcgc g
//
LOCUS       DATBFZ010000542        10950 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_4546962, whole genome shotgun sequence.
ACCESSION   DATBFZ010000542 DATBFZ010000000
VERSION     DATBFZ010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 10950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10950
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4546962"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            152..1255
                     /locus_tag="VH419_16540"
     CDS             152..1255
                     /locus_tag="VH419_16540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012918746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HEY7045280.1"
                     /translation="MSLSWSQALAWRLRQQLLEPVGTESVAGVVRRLGAVAALPDTAA
                     ELAVRTRRQRSRPGEVARALAEGRIIQTFAFRGATHLMTPEDGGVYLALRTASRMWER
                     PSWQTFYGLTPTDWPLFREAVRDALADGPKTRAELGAAVATRRRFRQLGSVLADGAWT
                     LLKPLAWQGDMSFGPPRDGQATFQRLDGNPRWAGVPDLDTAGPRAVAAYFRAYGPATP
                     ARVQYWLGDGLGAGRKRVQGWIAGFGDRLAAVEIDGESAYVLREDLDQLSSTPATSAV
                     RLLPARDQWVLGAGTKDAHVVPPARRSLVTGGANVVIVGGVVSGTWSLTDDQVVTAWF
                     AEAGRPPRQALETEVARLATILDRPLRSTLQPA"
     gene            complement(1289..2449)
                     /locus_tag="VH419_16545"
     CDS             complement(1289..2449)
                     /locus_tag="VH419_16545"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009074364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HEY7045281.1"
                     /translation="MIVVSVASGTSADGLDVAVVDLVWDGSTIQAALRHHATEPWPGG
                     LRERLLALLPPASTTAADVCELDTRIGQAVGAAAARVVDRFPETELVVSPGQTVHHEV
                     RHDRCLGTLQLGQPAWIAEATGLPVLSDLRARDVAAGGHGAPLASTFDALWLAGPKGP
                     RAALNLGGIANATVVGAPIDRAALAWDTGPANCLLDVASARATGGRLRFDEDGRLAAS
                     GSVRTDLREVLLAHPFFRLKPPKSTGRETFSAAYLDEVLSRFGDLRSGDLLATLTDLT
                     ATTVAQALAPYDVVEVVVSGGGARNPALMAALRQRLSPTPLLTSAELGLPVQAKEAVM
                     WALLGFLTWHAVPGTNGATGGRPRVLGRITPGSEPLVLPQPAGIVPTRLILG"
     gene            complement(2446..4401)
                     /gene="murQ"
                     /locus_tag="VH419_16550"
     CDS             complement(2446..4401)
                     /gene="murQ"
                     /locus_tag="VH419_16550"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: protein motif:HMM:NF009222.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HEY7045282.1"
                     /translation="MSVIIGVDLGKTSCRARIRVGDRSTDEVTGPGAIGLAEPEGVAD
                     ATTAVVTVVARALRLLGEDHASEGSVRVPRVETVVIGAAGAEAAAQSATRLANAVLDA
                     VGAVHVAVTSDAVIAHAGAFSGHAGSVVSIGTGAVAVAVDERGRLHRSDGWGPWLGDD
                     GSGAWIGREGLRAVLLAAEGRGGPTALTELAERRFGARRQLPEAVSAGNQVARTLASF
                     APDVLSAAATGDPAAEDVVGRAGRALAETVMTTARAAGVSLVTAVGGLTDASLLTDAW
                     RAALPSGVTVCRADGTAIDGALLMAGRTDLPHESQVLRVNSAAHLGRHRATSVDVTPR
                     SAGTGHEHVDVDGLATERVRPDLFDLDMREPEELVALLLEAEATVPDVMRSARPSLAA
                     AVRLVEAAFEAGGRLIYVGAGTPGRLAAVDAAECPPTFGTSPDQVVALLAGGNRAAVA
                     AVEGAEDDEVAGRSDLDGLEPGRHDVVVGITASGRTPYVLAALDHARARGAATVAIVN
                     NPGSPAKSHADVLVELPTGGEVLAGSTRLKAGTSQKVALNVLSTSAMIRSGKTYGAWM
                     VDVMASNEKLRRRARRILREATGVDDLTALQALDDCGWNTKTAMVSVLTGLDPDASRR
                     RLEEAGGHVRQAVSPAALASPAAQSEP"
     gene            complement(4398..5960)
                     /locus_tag="VH419_16555"
     CDS             complement(4398..5960)
                     /locus_tag="VH419_16555"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEY7045283.1"
                     /translation="MTRTGSVERLACAVTVPGFNGTTTPDWLARRIDEGLGGVCWFGH
                     NVPDVETARALSDDLHARRPDIVVLCDEEAGGVSRLEDRTGSSWPGAAALGRLDDVAA
                     TRDVAAAVGRLARKAGIDVVLAPVVDVNSDPDNPVIGVRSFGSEPELVGKHAAAFVDG
                     LQSSGVAACAKHYPGHGATRTDSHLDLPYVDDPEAVLRTRDIAPFAAVVEAGVRTVMT
                     AHVVFPAMDDQPATTSARLLGLLRDELGFGGVIVSDALDMHAISRRVGRGPGGVQAMA
                     AGVDLLCIGNPGFPAAYDAETVCDEVVDAIVRAVEDGSLTEERLADAAARVTALSQWL
                     DERPEAFGEADDDERFGLDVARRTISVRGDVRLRRTPFVVDVAGPVSIAAGHRHAHLL
                     DALVADAPGAEVCTLPSGAPESHVDEVLAAAGDRDVVIVVSSRLDPATVANVRTLVAA
                     APDAVVVSAGSAVEIDATGRGAVDTFGGGRATAVAVAEALGVAVLDDDPNGPPATADG
                     LDHTDSRQRGLA"
     gene            complement(5957..6784)
                     /locus_tag="VH419_16560"
     CDS             complement(5957..6784)
                     /locus_tag="VH419_16560"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEY7045284.1"
                     /translation="MSSRSRTAKVWKAVGVVAVLVFSLFPVFMMVSTAVDVHANDGSR
                     NLIPTSFTLDNFRFVLDEGNFTTYLRNSVIVSLGTVLISGLVALFASVGVARFRFRFR
                     TSVLVMILIVQMVPLEALVIPLFLQAKTLGLLNHLFGLVVVYVAFSLPFAIWTLRGFV
                     AAIPVEIEEAAYVDGASWFRMFWSILFPLVAPGLVAVSVFSFITAWNEFIFAITFMND
                     PEKYTVAAGLRQFFSQYGTNWGAVMAGSTLITLPVMLFFVVVQRKLSSGLASGAVKG"
     gene            complement(6781..7674)
                     /locus_tag="VH419_16565"
     CDS             complement(6781..7674)
                     /locus_tag="VH419_16565"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEY7045285.1"
                     /translation="MSVQVWRPWLLLTPALVVVAGLLIYPLYRVGVLSFQDFGLPELL
                     KGDAPWIGLDNFKEVLGDPLLWKTVLPNTVVFAAVNVALTVIVGTLVALLLASLGRWT
                     RGFVTSAIMVAWAVPAVTGTYVWIFLFDPGTGVFMRALAAIGLIDLDTTNWFTDRLTF
                     YAIATLNVVHHGFPFVAITVLAGLLTLPQETLEAARMDGAGAWRRFWYITVPLLKPVF
                     AVVTILSTIWDFKVFTQIYLMPGGNGTNREVLNLGTWSYVSALAQNRFGLGASIAVLL
                     TVVLLIITVVYLRVMFRQEEL"
     gene            complement(7823..9124)
                     /locus_tag="VH419_16570"
     CDS             complement(7823..9124)
                     /locus_tag="VH419_16570"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEY7045286.1"
                     /translation="MQIRSRRHHRRLAVGLAALGLLAAACGGNDSSDDSSSAKKLTVW
                     IMQGTNPDATPFFKDVTAAFKDQTGADLDVQFVEWADAHDKFISSIAGGTTPDVAEVG
                     TTWTPEFADAGALEDLTDQVDSAGLQSDLVPGLVEAGTIDGKLYGMPWYAGVRSVVYR
                     TDVFKKAGVEPPTTWDEFVQVADKVKAAEPDMTTFPVPGDSEYGLYPWIWGAGGEIAT
                     QNDDGTWTSTIDSAEAQQGTTFYTDLALKHGFSSPAATTWDEADLSDAFSRGDVAMMI
                     AGSWTPGALVEANPDLKGKIGAFPIPGPDGGLSPSFLGGSHLSIFNTADDPDLAWKFV
                     ELMTTGKFAAEWGEQSGFFPGTKSLLEKVQAENDPLVAPFAKQMAEAGKSVPVTPLYG
                     QIQGNKTVSAMLQSILSGKASVDEASKQAAQEMDDIFSRGS"
     gene            complement(9218..9958)
                     /locus_tag="VH419_16575"
     CDS             complement(9218..9958)
                     /locus_tag="VH419_16575"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY7045287.1"
                     /translation="MTTVESGRETAKQRQVRERLEQLISTLSPGEPLPVERDLAKDLG
                     VARMTLRRAVDALVLEQRLIRRQGSGTFVAAPKVTQRLSATSFSADMRARGLRPGSHT
                     VFARQAPAGMMLSSLLGVPPHANVLHVRRLRMADDEPMALEDLHVPSDIAPGLTGDDL
                     EDTSFYELLAQRYQRPIVAGTQTVEPCLVSREEAKLLHVEVGAPAFLFERTSRIADGR
                     VAEFVRSIYRGDRYRIVVDIFPQAPGSS"
     gene            complement(10017..>10950)
                     /locus_tag="VH419_16580"
     CDS             complement(10017..>10950)
                     /locus_tag="VH419_16580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020577667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HEY7045288.1"
                     /translation="VAGQAFPDDDTFAQYPDGYTDKAQWRRHNINLLITELADRIHDA
                     KPWVKFGVSPFAVWRNKATDPEGSDTTAGAQTYDDLYADTRRWVRDEWIDYIAPQVYW
                     NIGFAPADYAMLVPWWAQQVRGTDVQLYIGQATYKVELSTQDPAWQDPQEMTRHLFFN
                     RFFPEVQGDIYFSAKDVRANRLGNMDIVQADHYQHPALIPVTAGVPGHTPKAPSRLTS
                     SRGPDAVDLSWRGNSDSSVEYAVYRFDGAGVRPSRCDGADASHLVATVRGDATSTDQH
                     WTDVTAEPGATYTYVVSALDRAYRESAPSRPVTS"
BASE COUNT         1725 a         3814 c         3810 g         1601 t
ORIGIN      
        1 gctcgtcgtg agcggtgtcc gggtggatga gctgcaaggc agaggaggga ggcgatgcgc
       61 agcatcgttg accgacgata acgccgcaga tcgccgcccg ggcggcgcgc aacagggcga
      121 atgttggttg acggggtact aacatgcgct ggtgtccctc agctggtccc aagccctcgc
      181 ctggcggctg cgacagcagt tgctcgaacc ggtcggcacc gagtcggtcg caggtgtcgt
      241 acgccgcctc ggcgccgttg ccgctctccc cgacacagcc gccgagctcg ccgtccgcac
      301 caggcgccag cggtctcggc cgggcgaggt tgcacgcgcg ctggccgagg ggcggatcat
      361 ccagaccttc gcgttccgcg gcgccaccca cctgatgacc ccagaagacg gtggtgtgta
      421 cctcgccctg cggacggcca gtcggatgtg ggagcgcccc agctggcaga ccttctacgg
      481 gctgacgcca acggactggc cgctgtttcg cgaggcagtc cgtgacgcgc tcgccgacgg
      541 gccgaagacg cgggcggagc tgggggcggc ggtggctacc cgtcgccggt tccggcagct
      601 gggctccgtc ctcgccgatg gcgcctggac gctgctgaag ccgctggcgt ggcagggcga
      661 catgagcttc gggccgccgc gggatggcca ggcgacgttc cagcggctgg acggcaatcc
      721 gcgttgggcg ggcgtccctg acctcgacac ggccggcccg cgtgcggtgg cggcgtactt
      781 ccgggcctac ggtccggcga cgcctgcccg cgtccagtac tggctcggcg acggactggg
      841 cgcaggacgc aagcgggtcc agggctggat cgccggcttc ggtgaccgac ttgcggcggt
      901 cgagatcgac ggggagtccg cctacgtcct gcgcgaggat ctggaccaac tctcatcgac
      961 cccggcgacg tctgcggttc gcctgctgcc cgcgcgcgac caatgggtgc tcggtgcggg
     1021 cacgaaggac gcccacgtcg taccccctgc ccgtcggtcg ctggtcaccg gcggggcgaa
     1081 tgtcgtgatc gtcggtggtg tcgtgtcagg aacgtggtcg ttgaccgacg accaggtcgt
     1141 caccgcgtgg ttcgccgaag cgggacggcc gccgcggcag gcgctcgaga ccgaggttgc
     1201 gcgactcgcc acgatcctcg accgacccct tcggtccacg ctccagccgg cgtgagcgac
     1261 cgccatcgcc gacgctcgcc gagtcaggtc accccaggat caaccgggta gggacgatgc
     1321 cggcgggttg tggaaggacg agcggctcgc tgcccggggt gatgcggccg aggacacgtg
     1381 gtcggccgcc ggtcgcgccg ttcgtccctg gtactgcgtg ccaggtgagg aagccgagca
     1441 acgcccacat caccgcctcc ttggcctgca ccggaagccc gagttcggca ctcgtcaaca
     1501 gcggggtagg gctcaaccgc tgccgcaggg ccgccatcag tgccgggttg cgggcacctc
     1561 caccggacac caccacttcg acgacgtcgt acggcgccaa cgcctgcgcg accgtcgtcg
     1621 ccgtcaggtc cgtgagcgtc gccagcaggt ccccggatcg gaggtcgccg aagcgggaca
     1681 gcacctcgtc gaggtacgcc gccgagaacg tctcccgtcc ggtcgacttc ggtggcttca
     1741 gccggaagaa cggatgagcc agcagcacct cgcgcaggtc ggtgcgcacc gagccggacg
     1801 cggcaagccg tccgtcctcg tcgaaacgaa ggcgaccgcc ggtcgcccgc gctgacgcga
     1861 cgtcgagcag acagttcgcg gggccggtgt cccacgccag ggcggcacga tcgatgggcg
     1921 ctcccaccac ggtggcgttc gcgatcccac ccaggttcaa ggcggcgcgg ggtccctttg
     1981 ggccggcgag ccagagcgcg tcgaacgtgc tggcgagcgg cgcgccatgg ccccccgcgg
     2041 cgacgtcgcg cgctctcagg tcggacagca ccggaagccc ggtcgcctcc gcgatccagg
     2101 cgggctgacc gagctggagc gtgccgaggc agcggtcgtg ccggacctcg tgatgcacgg
     2161 tctgacccgg agacacgacc agctccgtct cggggaagcg atcgacgacc cgtgccgcag
     2221 cggccccgac agcctggccg atgcgggtgt cgagctcaca cacatcggcg gcggtcgtcg
     2281 atgccggtgg cagcaacgcc agcagtcgct cacgcagccc gcccggccac ggctcggtcg
     2341 cgtggtggcg gagggccgcc tggatcgtcg agccgtccca gaccagatcc acgaccgcga
     2401 cgtcgagacc gtcggccgag gttccggagg cgacggagac gacgatcacg gctcggactg
     2461 agcggccggc gaggccagcg ccgcaggcga gaccgcctgt cggacgtgtc cacccgcctc
     2521 ctcgagccgt cgccgcgacg cgtccggatc gagcccggtg aggacggaca ccatcgccgt
     2581 cttggtgttc cagccacagt cgtccaacgc ctgcaacgcg gtgaggtcgt cgacgccggt
     2641 cgcctcacga aggatccgcc gcgcacgtcg gcgaagcttc tcgttcgacg ccatcacgtc
     2701 gaccatccac gcgccgtacg tcttgccgga ccggatcatc gcgctggtcg acaacacgtt
     2761 gagtgcgacc ttctgcgacg tacccgcctt cagccgggtg gaccccgcca gcacctcacc
     2821 accggtcggg agctcgacca gcacgtcggc gtggctcttc gccggactgc ccgggttgtt
     2881 gacgatcgcg accgtcgcgg ctccgcgcgc ccgggcatgg tcgagcgccg ccagcacgta
     2941 cggtgtgcgg cccgatgcgg tgatgccgac gacaacatcg tgccgccctg gttccagccc
     3001 gtcgaggtcg ctgcgcccgg ccacttcgtc atcctcggcg ccttcgacgg cggcgaccgc
     3061 cgcgcggttg ccgccggcca gcagcgccac gacctggtcc ggcgatgtgc cgaacgtcgg
     3121 tgggcactcg gccgcgtcga cggctgccag ccggcctggg gtcccggcac ccacgtagat
     3181 cagccgcccg cccgcttcga acgctgcttc cacgagccgc actgcggcgg ccagcgacgg
     3241 ccgtgccgac cgcatcacgt ccggaaccgt ggcttcggct tccaacagca gggcgacgag
     3301 ctcttccggc tcacgcatgt cgagatcgaa caggtcagga cgcacccgtt cggtggcgag
     3361 gccgtcgacg tcgacgtgtt cgtggccggt gccggccgac ctcggcgtga cgtcgaccga
     3421 ggtcgcccgg tgccggccaa ggtgcgcagc ggagttgacc cggagcacct gggactcgtg
     3481 cggcagatcg gttcgaccgg ccatcagcag cgctccgtcg atggccgtcc cgtcggcccg
     3541 gcagacggtc acgccggacg gcagggcggc gcgccaggca tccgtcaaca ggctcgcgtc
     3601 cgtcagcccg ccgacggcgg tgaccagcga cactccggcc gcccgcgcgg tcgtcatcac
     3661 ggtctcggcg agcgcacgac cggctcggcc cacgacgtcc tcggctgccg ggtcaccggt
     3721 cgcggccgcg gacagcacgt cgggtgcgaa gctcgccagc gtccgcgcca cctggttgcc
     3781 ggccgatacc gcctctggca gctgacggcg cgcaccgaaa cggcgttcgg ccagctcagt
     3841 gagcgccgtc ggtccgccgc ggccctccgc ggccaacagc accgcacgca gtccttcacg
     3901 cccgatccag gcgccactgc cgtcgtcgcc gagccagggt ccccagccgt ccgatcggtg
     3961 cagccttccg cgctcgtcga cggcgaccgc caccgcgccg gtgccgatcg agacgacgct
     4021 tcccgcgtga ccggagaacg cacccgcatg ggcgatcacc gcatcggagg tcaccgccac
     4081 gtggacagcg ccgacggcgt cgaggacagc gttggcgagt ctggtggccg actgcgcggc
     4141 tgcttcggcg ccggccgctc cgatcaccac ggtctcgact cgcggcaccc gaacagagcc
     4201 ttcggaggcg tgatcctccc cgagcagtcg cagtgcgcgg gccacgaccg tcaccaccgc
     4261 cgtggtggca tccgcgaccc cttccggctc tgccagaccg atggcgcccg gtcccgtcac
     4321 ctcatcggtc gaccggtcgc cgaccctgat ccgggcccgg cagctcgtct tcccgaggtc
     4381 gacgccgatg atcacgctca tgccagtcct cgttgtcggc tgtcagtgtg gtcgaggccg
     4441 tccgctgtcg cgggaggccc gttcgggtca tcatcgagca cggcgacacc cagcgcctcg
     4501 gcgacggcga cggccgtcgc tcgtcccccg ccgaaggtgt ccacggcccc ccggccggtc
     4561 gcatcgatct cgacggcact cccggcggag acgacaaccg cgtcgggggc tgccgcgaca
     4621 agggtgcgca cgttcgcgac cgtcgccggg tccagccgcg acgacacgac gatcaccacg
     4681 tcgcgatcac cggctgcagc cagcacctcg tcgacgtgtg actccggggc gccgctcggc
     4741 agtgtgcaga cctccgcacc cggtgcgtcc gccacgagtg cgtccagcag gtgcgcgtgc
     4801 cgatggccgg cggcaatgct cacgggacct gcgacgtcga cgacgaacgg cgtacggcga
     4861 aggcgcacgt ccccacggac ggagatcgtg cgtcgtgcca cgtcgagacc gaaccgctcg
     4921 tcgtcatcag cttcgccgaa tgcttccggg cgttcgtcga gccattggga cagcgcggtc
     4981 acacgggcgg ccgcgtcggc cagccgctcc tccgtgaggc tcccgtcctc gacggctcgc
     5041 acgatagcgt ccacgacctc gtcgcagacg gtctccgcgt cgtacgccgc cgggaagccc
     5101 gggttgccga tgcacagcag gtcgacaccg gccgccatcg cctgcacccc acccggtccg
     5161 cggccgaccc gacgggagat cgcgtgcatg tccagcgcgt cggagacgat cacgccgccg
     5221 aacccgagct cgtcgcgcag cagtccgagc agccgcgcgc tcgtcgtcgc gggctgatcg
     5281 tccatcgcgg ggaagacgac gtgggcggtc atcaccgtgc gcacaccggc ttccaccacg
     5341 gccgcgaagg gtgcgatgtc gcgcgtgcgc agcacggcct ccgggtcgtc cacgtacggc
     5401 aggtcgaggt gcgagtcggt ccgggtggct ccgtgacccg ggtagtgctt ggcgcacgca
     5461 gcgacgccgc tggactggag accgtcgacg aaggcggctg cgtgcttgcc cacgagctcc
     5521 ggctcggacc cgaacgagcg cacgccgatg accgggttgt cgggatcgga gttgacgtcc
     5581 acgacgggtg cgagcacgac gtcgatccct gccttccggg ccagcctgcc gacggcggcg
     5641 gccacgtcgc gagtggcagc gacgtcgtcg agacgaccga gggcggcagc gcccggccat
     5701 gacgagccgg tgcggtcctc gagccggctc acaccgcccg cctcctcgtc acacagcacg
     5761 acgatgtccg ggcgcctggc gtgcaggtcg tcggacaatg cgcgcgccgt ctcgacgtcc
     5821 ggcacgttgt gcccgaacca gcagaccccg ccgagaccct cgtcgatgcg gcgggcgagc
     5881 cagtccgggg tcgtggtgcc gttgaacccc ggcaccgtca cggcgcaggc gaggcgttcg
     5941 accgatccgg tacgcgtcat cccttcacgg cccccgaagc caggccggac gagagcttgc
     6001 gctgcaccac cacgaagaag agcatgacgg gcagcgtgat gagcgtcgac cctgccatca
     6061 cggcacccca gttcgtcccg tactgcgaga agaactgtct gagtccggcc gccaccgtgt
     6121 acttctccgg gtcgttcatg aacgtgatgg cgaagatgaa ctcgttccac gccgtgatga
     6181 acgagaagac gctcaccgcg accaacccgg gagctaccag cgggaagagg atcgaccaga
     6241 acatccgaaa ccaggaggcc ccgtcgacgt acgccgcctc ctcgatctcg accgggatgg
     6301 cggcaacgaa gccgcgcagg gtccagatgg cgaacggcag cgagaacgcc acgtacacga
     6361 cgacgagtcc gaacagatgg ttgaggagtc cgagcgtctt cgcctgcagg aagagcggga
     6421 tgaccagcgc ctccaacggc accatctgca cgatcaggat catcaccaga acggaggtcc
     6481 ggaaccggaa ccggaagcgt gccaccccga ccgaggcgaa cagcgcgacg agcccgctga
     6541 tcaggaccgt tccgagcgag acgatcaccg agttgcgcag gtacgtggtg aagttcccct
     6601 cgtcgagcac gaaccggaag ttgtcgagcg tgaacgacgt cgggatgagg ttgcgggagc
     6661 cgtcgttggc gtgcacgtcc accgccgtcg acaccatcat gaacaccggg aagaggctga
     6721 agaccaggac ggcgacgaca ccgaccgcct tccacacctt cgcggttctg gaccggctgc
     6781 tcacagttcc tcctgtcgga acatcacccg caggtagacg acggtgatga tcagcaggac
     6841 gacggtgagc aggaccgcga tggacgcgcc gaggccgaag cggttctgcg cgagcgcgga
     6901 gacgtacgac caggtgccga ggttgaggac ctcgcggttc gtgccgttgc caccgggcat
     6961 gaggtagatc tgcgtgaaga ccttgaagtc ccagatcgtc gacaggatcg tcacgaccgc
     7021 gaacaccggc ttcaacaggg gcacggtgat gtaccagaag cgtcgccagg cgcccgcgcc
     7081 gtccatccga gcggcctcga gcgtctcctg cgggagcgtg agcagccccg ccagcacggt
     7141 gatggccacg aacgggaagc cgtggtggac gacgttgagg gtggcgatcg cgtagaaggt
     7201 gagccggtcg gtgaaccagt tcgtcgtgtc caggtcgatc aggccgatcg cggcgagcgc
     7261 gcgcatgaag acaccggtgc cggggtcgaa caggaagatc cacacgtagg ttccggtgac
     7321 ggcagggacc gcccaggcga ccatgatcgc gctcgtcacg aaaccccgcg tccagcggcc
     7381 gaggctcgcc aagagcaacg cgaccagcgt gccgacgatg acggtgagtg cgacgttcac
     7441 ggcggcgaag acgacggtgt tgggcagcac cgtcttccac agcagcgggt cgccgagaac
     7501 ctccttgaag ttgtccaggc cgatccacgg ggcatcgccc ttgagcagct cgggcagacc
     7561 gaagtcctgg aacgacaaga cgcccactcg atagagcggg tagatgagca gtccggcaac
     7621 gacgacgagc gccggcgtca agagaagcca gggacgccac acctggacgc tcaccggacc
     7681 cctgcccatc tcccggaaga gatggcggaa tctcggggaa cgcccaggtg tggtcaccgc
     7741 cggtcgtggg tgcgagtcat ctggcggtga ggggatcagt gccgagccct ggttcgggct
     7801 cggcccctgg accatgtccc gatcaactgc cacgagagaa gatgtcgtcc atctcctggg
     7861 cagcctgctt actggcctcg tcgacgctgg ccttgccgga aaggatcgac tgcagcatcg
     7921 ccgagacggt cttgttgccc tggatctggc cgtacagcgg cgtcaccggg acggacttgc
     7981 ccgcttctgc catctgcttg gcgaacgggg ccacgagcgg gtcgttctcg gcctgcacct
     8041 tctccagcag cgacttcgtg ccggggaaga agccggactg ctcgccccac tcggcagcga
     8101 acttgccggt ggtcatcagc tcgacgaact tccaggccag gtccgggtcg tcggcggtgt
     8161 tgaagatcga caggtgcgac ccaccgagga acgaggggct cagcccaccg tccggaccag
     8221 ggatcgggaa ggcgccgatc ttccccttca ggtctgggtt ggcctcgacg agcgctccgg
     8281 gtgtccagct gccggcgatc atcatcgcga cgtcaccgcg ggagaacgcg tcactgaggt
     8341 cggcttcgtc ccatgtcgtc gcggcggggg acgagaagcc gtgcttcagt gcgagatcgg
     8401 tgtagaaggt cgtgccctgc tgagcctcgg cagagtcgat cgtcgacgtc caggtgccgt
     8461 cgtcgttctg ggtggcgatc tctccgcccg caccccagat ccacgggtac aggccgtact
     8521 cgctgtcacc gggaaccggg aaggtggtca tgtcgggctc agcggccttg accttgtccg
     8581 ccacctgcac gaactcgtcc caggtcgtcg gcggctctac gccggccttc ttgaagacgt
     8641 ccgtccgata cacgacggac cgcacaccgg cgtaccacgg catgccgtag agcttgccgt
     8701 cgatcgtgcc ggcctcgacg agtccgggta ccaggtcgct ttgcagaccc gcggagtcga
     8761 cctggtcggt gaggtcctcc agcgcacccg cgtcagcgaa ctccggtgtc caggtcgtgc
     8821 ccacctcggc gacgtcaggg gtcgtgccgc ccgcgatgga gctgatgaac ttgtcgtggg
     8881 cgtccgccca ctcgacgaac tggacgtcga ggtcggcgcc ggtctggtcc ttgaacgccg
     8941 ccgtcacgtc cttgaagaac ggcgtcgcgt ccgggttggt cccctgcatg atccagacgg
     9001 tcagcttctt tgccgacgac gaatcatccg aggagtcgtt gccaccgcac gcagccgcga
     9061 gcagaccgag agcggccagc ccgacagcga ggcgccggtg atggcggcgg gaacggatct
     9121 gcatggtgcg ggtcctttcg aaggagggtg ctcttcgagc cgaggcagac cataggtcta
     9181 gacctccgga cgattcttga cctcgctggc ccgtccgtca agagctgccg ggcgcttgcg
     9241 ggaagatgtc gaccacgatc cgataacggt cgccacgata gatcgaccgc acgaactccg
     9301 cgactcgacc gtccgcgatc ctcgacgtgc gctcgaacag gaaggcgggt gcgccgacct
     9361 cgacgtggag gagcttcgcc tcctcccgcg agaccaggca gggctcgacc gtctgggtac
     9421 ccgcgacgat cgggcgttgg tagcgctgcg cgagcagctc atagaacgac gtgtcctcga
     9481 ggtcgtcgcc ggtgagtccg ggcgcgatgt cagacggcac gtgcaggtcc tccaacgcca
     9541 tgggctcgtc gtcggccatc cgcaaccgcc gcacgtgcag gacgttcgcg tgcggcggta
     9601 cgccgagcaa cgacgacagc atcatcccgg ccggcgcctg ccgggcgaag acggtgtggc
     9661 tgcccgggcg caggccgcgg gcgcgcatgt cggcggagaa cgacgtggcc gacagccgtt
     9721 gcgtcacctt cggcgcggcg acgaacgtgc ccgagccctg ccggcggatg agccgctgct
     9781 cgagcacgag ggcgtcgacc gcccgccgca acgtcatccg cgcgacaccc aggtccttgg
     9841 cgaggtcgcg ctcgaccggc aacggctcgc ccggcgacaa ggtggagatg agctgctcga
     9901 gtcgctcacg cacctgccgt tgcttggccg tctcgcgtcc ggactcgacg gtggtcatgg
     9961 accattggta caggaaatgg ccggcagacg ccagcacccg gcgggagcgc gcgccatcac
    10021 gaggtcaccg gccggctcgg agcgctctcc cggtacgcgc gatcgagagc gctgacgacg
    10081 tacgtgtacg tcgcccctgg ctccgcggtg acatcggtcc agtgctggtc ggtcgacgtc
    10141 gcgtcacctc gcaccgttgc gaccaggtgg ctggcgtcgg cgccatcaca tcgggacggc
    10201 cggacacctg cgccgtcgaa gcggtagacg gcgtactcga cggagctgtc cgagttgccg
    10261 cgccagctca ggtccacggc gtcgggtccg cgcgacgagg tcaaccggct cggtgccttc
    10321 ggtgtgtgac cgggcacgcc ggcggtgacc gggatcaggg ccgggtgctg gtagtggtcg
    10381 gcctgcacga tgtccatgtt gccgagccgg ttggcgcgga cgtccttggc ggagaagtag
    10441 atgtcgcctt gcacctcagg gaagaagcgg ttgaagaaca ggtgccgggt catctcctgc
    10501 gggtcctgcc atgccgggtc ctgggtcgac agctccacct tgtacgtcgc ctggccgatg
    10561 tagagctgga cgtcggtgcc gcggacctgc tgagcccacc aggggacgag catcgcgtag
    10621 tcggccggtg cgaagccgat gttccagtag acctgcggcg cgatgtagtc gatccactcg
    10681 tcacggaccc agcgcctggt gtcggcgtac aggtcgtcat acgtctgggc gccggccgtc
    10741 gtgtcggagc cctcagggtc ggtggccttg ttgcgccaca ccgcgaaggg gctgacgccg
    10801 aacttcaccc agggcttggc atcgtggatg cggtcggcga gctcggtgat gagcaggttg
    10861 atgttgtgcc gccgccattg ggccttgtcg gtgtaaccgt cggggtactg cgcgaacgtg
    10921 tcgtcgtccg ggaacgcctg gcctgccacc
//
LOCUS       DATBFZ010000543         2395 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_3890285, whole genome shotgun sequence.
ACCESSION   DATBFZ010000543 DATBFZ010000000
VERSION     DATBFZ010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3890285"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="VH419_16585"
     CDS             complement(<1..293)
                     /locus_tag="VH419_16585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009083592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY7045289.1"
                     /translation="MRILVVDDDRAVRDSLRRSLEFNGYEVALANDGVEALARINGLA
                     PDALIVDVMMPRLDGIETTRALRAAGNDLPILVLTARDSVNDRVDGLDAGADD"
     gene            complement(352..1230)
                     /locus_tag="VH419_16590"
     CDS             complement(352..1230)
                     /locus_tag="VH419_16590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020577963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HEY7045290.1"
                     /translation="MSTIAARAEALLGTAVVATTPVAGGDVCTATRLRLSDGRSAFVK
                     TRPHAPTGFFAAEARGLDWLADGDGAAVPKVLGVSDDCLILSWIEPGRPSLDTAEAFA
                     RQLAATHTSGADRFGGEQDGYIGTLPLTNAPADTWTTFWATRRVLPYLKVARDRDHIT
                     DGDAAAVEEVIEQIDEYAGPPEPPSRIHGDLWSGNIVWAAEGPAYLVDPAAHGGHRET
                     DLAMLALFGAPHLSRLLEAYDEAAVLADGWRERQPLHQLHPLLVHAILFGGSYGSRAG
                     AAARSLLDGTSGHLPG"
     gene            complement(1314..1853)
                     /locus_tag="VH419_16595"
     CDS             complement(1314..1853)
                     /locus_tag="VH419_16595"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007079394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HEY7045291.1"
                     /translation="MTSPTAQLLPEPSDAGSAYTIALVCLGNICRSPTAQVVLTKKLA
                     DAGLDEKVTVRSAGTGTWHIDEAMDHRSAALLTAGGYDPTLHRATLFDKTWFGQYDLV
                     LVMDHHNLRDVQQLATTAADMERIMMFRAFDPAADESLDVPDPWFGDEDRFEDVLTIV
                     ERTSDALVTALRERLATNS"
     gene            complement(1850..2242)
                     /locus_tag="VH419_16600"
     CDS             complement(1850..2242)
                     /locus_tag="VH419_16600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008354652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEY7045292.1"
                     /translation="MKAWLIRIFTNACALAVATWLFDGIDITGHNDRNRVLTLIVVAF
                     LFGVVNEFVRPVVAFLSIPLYLITLGLMYFVVNALMLLLTSWICDWLDIGFHVDGFWT
                     AVWGGIVIAIVSWIVSQLLPEPDGHGWR"
BASE COUNT          383 a          805 c          830 g          377 t
ORIGIN      
        1 tagtcgtcgg cgcccgcgtc caggccatcc acgcggtcgt tgaccgagtc acgtgcggtc
       61 agcaccagga tggggaggtc gttgccggcg gcgcggaggg cccgcgtcgt ctcgatgccg
      121 tcgagccgag gcatcatgac gtcgacgatc agagcgtccg gggcgagacc gttgatgcgt
      181 gcgagcgcct cgacgccgtc gttcgcgagc gccacctcgt agccgttgaa ctccaacgaa
      241 cgccgcagcg agtcgcgtac ggcgcggtcg tcatccacca cgaggatgcg catggctcaa
      301 gcctgccacg tacttctgag accgcgctga gaggttgcgg atccgtgccg gtcaccccgg
      361 cagatggccc gacgtgccgt ccagcagcga ccgtgctgcg gcaccggcgc gcgatccgta
      421 ggagccaccg aagaggatcg cgtggacgag gagtggatgg agctggtgca agggctgccg
      481 ttcgcgccag ccgtcggcga ggaccgcggc ctcgtcgtac gcctccagca gccgcgacag
      541 gtggggggca ccgaacagcg cgagcatcgc caggtcggtc tctcggtggc cgccgtgcgc
      601 cgctgggtcg accaggtacg ccggcccttc cgccgcccag acgatgttgc cggaccacag
      661 atcgccgtgg atacggctcg gcggctcggg cgggccggca tactcgtcga tctgctcgat
      721 cacctcctcg accgcggcgg cgtcgccgtc ggtgatgtgg tcccggtcgc gggcgacctt
      781 gaggtagggc agcaccctgc gggtggccca gaacgtcgtc caggtgtcgg ccggcgcgtt
      841 ggtgagcggc agtgtgccga tgtagccgtc ctgctcgcca ccgaagcggt cggctcccga
      901 cgtgtgggtg gcggccagct ggcgtgcgaa tgcctcggcg gtgtccaggc tcggccgccc
      961 cggctcgatc caggacaaga tcaagcagtc gtcgctcacg cccagaacct tcggaacagc
     1021 agcgccgtcg ccgtcggcga gccaatccag gccgcgggcc tcggccgcga agaaccctgt
     1081 tggtgcgtgc ggccgggtct tcacgaacgc gctgcgcccg tcgctgaggc gcagccgcgt
     1141 tgcggtacag acgtcgccac cggccaccgg cgtggtggcg acgaccgccg taccgagcag
     1201 cgcctccgca cgcgctgcga tcgtgctcat ccgggccact ccccgaacct atcgcgcgaa
     1261 cgccatccac cgatcgagcc gtcgagaccg tcggccgtgc ggcagccggc cactcaggag
     1321 ttggtcgcga ggcgttcgcg gagcgcagtg acgagcgcgt cgctggtgcg ctcgacgatc
     1381 gtgaggacgt cctcgaaccg gtcctcgtcc ccgaaccagg ggtcggggac gtccagtgac
     1441 tcgtcagctg ccgggtcgaa ggcacgaaac atcatgatgc gctccatgtc ggcagccgtc
     1501 gtggccagtt gctggacgtc tcgcaggttg tggtggtcca tcaccagcac gaggtcgtac
     1561 tggccgaacc aggtcttgtc gaacaaggtc gcccggtgca gcgtcgggtc gtatcccccg
     1621 gcggtcagca gtgcagctga gcggtggtcc atcgcctcat cgatgtgcca ggtgccggtg
     1681 ccggcactgc gcacggtgac cttctcgtcc agtcccgcgt cggcgagctt cttggtcagt
     1741 acgacctggg cggtcggcga tcggcagatg ttgccgaggc agaccaacgc gatggtgtac
     1801 gccgaaccgg cgtcgctcgg ttcgggaagc agctgcgccg tcggagacgt catcgccagc
     1861 cgtggccgtc cggctccggc agcaactggc tcacgatcca gctcacgatg gcgatgacga
     1921 tcccacccca cacggccgtc cagaacccgt cgacgtggaa gccgatgtcg agccagtcgc
     1981 agatccagct cgtcagcagc agcatcaagg cgttgacgac gaaatacatc agcccgagcg
     2041 tgatcaggta gagcgggatc gacaagaagg cgacgaccgg gcggacgaac tcgttcacca
     2101 caccgaacag gaacgccacg acgatcaacg tgaggacgcg gttgcggtcg ttgtgtccgg
     2161 tgatgtcgat gccgtcgaac agccaggtgg cgacggcgag cgcacacgcg ttcgtgaata
     2221 tccggatgag ccaggccttc atgatcccga ccctagacga gtgattccaa gggaggttgc
     2281 ctggctcgcg gctccccgca tcccgcctgg ctgcgttgtc gtcactccga ataggagctc
     2341 cgctatgagt cgctcctccg ccttgccaga tcgggcgcgg atgagccgct cgctt
//
LOCUS       DATBFZ010000544         2018 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_5801514, whole genome shotgun sequence.
ACCESSION   DATBFZ010000544 DATBFZ010000000
VERSION     DATBFZ010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5801514"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2018
                     /gene="secA2"
                     /locus_tag="VH419_16605"
     CDS             <1..>2018
                     /gene="secA2"
                     /locus_tag="VH419_16605"
                     /inference="COORDINATES: protein motif:HMM:TIGR04221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="accessory Sec system translocase SecA2"
                     /protein_id="HEY7045293.1"
                     /translation="MATGEGKTLAGAIAAAGYAIGGRHVHVITINDYLARRDAEWMTP
                     LLEALGLTVGWITADSTAEQRRAAYECNVTYASVNEIGFDVLRDQLVTDVADLVSPNP
                     DVALIDEADSVLVDEALVPLVLAGTSHREQPRVEVIRMVGDLISGPDSHENFATDDDN
                     RNVHLTEAGARRLESKLGNIDLYSEEHVATTLTEVNVALHAHVLLQRDVHYIVRDGAV
                     HLINASRGRIASLQRWPDGLQAAVEAKEGIETTETGEVLDPITVQALVNRYPTVCGMT
                     GTALAAGEQLRQFYKLGVSPIPPNKPNIREDETDRVYLTEAAKNHAIVEHIAEVHETG
                     QPVLVGTHDVAESEDLHRRLVKAGVPAVVLNAKNDAEEAAVIAEAGKLGSVTVSTQMA
                     GRGTDIRLGGSDAGDDSAEKEKVAELGGLHVVGTGRHHTERLDNQLRGRAGRQGDPGS
                     TVFFSSWEDEVVASHLDDTKLPTETDEDGRILAPKAAGLLDHAQRVAEGRLLDVHANT
                     WRYNQLIAQQRAIIVDRRETLLRTDTARQELKDRSPERYAALAEELGEDGEEKLEKIC
                     RLIMLYHLDRGWCDHLAFLADIRESIHLRALGRQNPLDEFHRMAVDAFASLAADAIEA
                     AQQTFDTADSIEDEPGVDLSKLARPTSTWTYMVHDNPLNDDTMSALSL"
BASE COUNT          361 a          721 c          673 g          263 t
ORIGIN      
        1 gatggccacc ggtgagggca agaccctggc cggggcgatc gccgcggccg gctatgccat
       61 cggcggcagg cacgtccacg tcatcaccat caatgactac ctggcgcgtc gcgacgccga
      121 gtggatgacc ccgttgctgg aggcgttggg gctcacggtc ggctggatca ccgccgattc
      181 cacggccgag cagcgccgcg cggcctatga gtgcaacgtc acctatgcct cggtcaacga
      241 gatcggtttc gacgtgctgc gcgaccagct ggtcaccgac gtcgccgacc tggtttcgcc
      301 gaatcccgac gtggccctga tcgacgaggc cgactcggtg ctggtcgacg aggcgctggt
      361 gccgctggtg ctggccggca ccagccaccg tgaacagccg cgcgtcgagg tcatccggat
      421 ggtcggcgac ctgatcagcg gacccgactc gcacgagaac ttcgcgaccg acgacgacaa
      481 ccgcaacgtg cacctcaccg aagcgggtgc gcggcgcctc gaatccaaac tcggcaacat
      541 cgacctgtac tccgaggaac acgtcgccac cacgctcacc gaggtcaacg tcgcactgca
      601 cgcgcatgtg ctgttgcagc gcgacgtgca ctacatcgtc cgcgacggcg cggtccacct
      661 gatcaacgcg tcccgcgggc ggatcgcctc gctgcagcgc tggccggacg gcctgcaggc
      721 cgccgtcgag gccaaggagg gcatcgagac caccgagacc ggtgaggtgc tcgaccccat
      781 cacggtgcag gcgctggtca accggtaccc gacggtgtgc ggcatgaccg gtaccgcgct
      841 ggccgccggc gagcagctgc gccagttcta caagctcggc gtgtcgccca tcccgccgaa
      901 caagcccaac atccgcgagg acgagaccga ccgggtctac ctcaccgagg cggccaagaa
      961 ccacgccatc gtcgagcaca tcgccgaggt gcacgagacc ggtcagccgg tgctcgtcgg
     1021 cacccacgac gtggccgagt ccgaggacct gcaccgcagg ctggtcaagg ccggtgtccc
     1081 cgccgtcgtg ctcaacgcca agaacgacgc cgaagaggca gccgtgatcg ccgaggccgg
     1141 caagctgggc tcggtgacgg tgtccaccca gatggccggc cgcggcaccg acatccggct
     1201 gggcggatcc gacgccggcg acgactcggc ggagaaggag aaggtggccg aactcggcgg
     1261 gctgcacgtc gtcggcacgg gccgtcacca caccgaacga ctggacaacc agctgcgcgg
     1321 gcgcgcgggc cgtcagggcg accccggctc gacggtgttc ttctccagct gggaggacga
     1381 ggtcgtggcg tcgcacctcg acgacaccaa gctgccgacc gagaccgacg aggacggccg
     1441 catcctggcg cccaaggcgg ccggtctgct cgaccacgcg cagcgggtcg ccgagggccg
     1501 gctgctcgac gtgcacgcca acacgtggcg ctacaaccag ttgatcgccc agcagcgcgc
     1561 catcatcgtg gaccgtcgcg aaaccctgct gcgtaccgac accgcacgcc aggagctgaa
     1621 ggaccgttca cccgagcgct acgccgcgct ggccgaggaa ctgggggagg acggcgagga
     1681 gaagctggag aagatctgcc ggctgatcat gctctaccac ctggaccggg gctggtgcga
     1741 ccacctggcg ttcctcgccg acatccggga gagcatccac ctgcgggcgc tgggccgcca
     1801 gaatccgctc gacgagttcc accggatggc cgtcgacgcg ttcgcctcgt tggccgccga
     1861 tgccatcgag gccgcccagc agaccttcga caccgccgac tcgatcgagg acgaaccggg
     1921 cgtcgacctg tccaagctcg cccggccgac atcgacgtgg acgtacatgg tccacgacaa
     1981 tccgctcaac gacgacacca tgtcggcgct gagcctgc
//
LOCUS       DATBFZ010000545        10578 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_5219465, whole genome shotgun sequence.
ACCESSION   DATBFZ010000545 DATBFZ010000000
VERSION     DATBFZ010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 10578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10578
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5219465"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..1250)
                     /gene="metK"
                     /locus_tag="VH419_16610"
     CDS             complement(57..1250)
                     /gene="metK"
                     /locus_tag="VH419_16610"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017933571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEY7045294.1"
                     /translation="MTGRLFTSESVTEGHPDKIADQISDSILDALLEQDPRSRVAVET
                     LVTTGLVVVAGEVTTEAYAPIADIVRDRILDIGYDSSKKGFDGASCGVQVAIDKQSKD
                     IAQGVDDAYEERHDHSSDPLDRQGAGDQGLMFGYACDDTPELMPLPITIAHRLAERLT
                     TVRKDGTMPYLRPDGKTQVTIEYDGDRPVRVDTVVISSQHAADIDLERLLTPDVKKHV
                     VDEVLSHFDVDASDYTLLVNPTGRFEVGGPMGDAGLTGRKIIIDTYGGMARHGGGAFS
                     GKDPSKVDRSGAYAMRWVAKNVVAAGLARRCEVQVAYAIGKAHPVGVYVECFGTETVE
                     VDAIRAAVLEVFDLRPAAIIRDLDMLRPIYALTAAYGHFGRDLPEFTWERTDRVAELK
                     AAVRS"
     gene            complement(1373..2608)
                     /gene="coaBC"
                     /locus_tag="VH419_16615"
     CDS             complement(1373..2608)
                     /gene="coaBC"
                     /locus_tag="VH419_16615"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009075820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEY7045295.1"
                     /translation="MSDQSRPRVLLGVSGGIAAYKACELLRRFTESGHDVTVVPTESA
                     LRFVGAATWEALSGHPVASGVFENVDTVQHVRLGQTADLVVVAPATADLLARAVHGRA
                     DDLLAATLLTARCPVVMAPAMHTEMWEHPATQANVATLRGRGVLVIEPAYGRLTGKDT
                     GPGRLPEPDEIFSVATDMLHRGQAGLEQDLRGRRVLVSAGGTREYLDPVRFLGNRSSG
                     KQGYALARAAVARGADVTLVAANVDLPDPAGAKVVQVVSALDLQEAMVAAAAAADVIV
                     MAAAPADFRPAVRVTHKIKKQPDGVTPDVPLVENPDILADLVARRSRSTSQVLVGFAA
                     ETGDATGSVLDHARRKLARKGCDLLVVNDVSAGAVFGEDDNQAVILGREGSQRAVPRG
                     SKAALAHVVWDCVVERLAM"
     gene            complement(2643..2921)
                     /gene="rpoZ"
                     /locus_tag="VH419_16620"
     CDS             complement(2643..2921)
                     /gene="rpoZ"
                     /locus_tag="VH419_16620"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012921921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEY7045296.1"
                     /translation="MSGSLPVAEGITHPPIDSLLEKTDSKYKLVLYSAKRARQINAYY
                     SQLGEGLLEYVGPLVDTHVQEKPLSIALREIDSDLLTCEDVEPESDEA"
     gene            complement(2933..3565)
                     /gene="gmk"
                     /locus_tag="VH419_16625"
     CDS             complement(2933..3565)
                     /gene="gmk"
                     /locus_tag="VH419_16625"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEY7045297.1"
                     /translation="MLAGPTAVGKGTVSAQIRAEYSDIWISVSVTTRPARPGEVDGVH
                     YRFVDDAEFDAMIERDELLEWAVVHKAARYGTPRAPVEKAVRDGRLALLEIDLQGARQ
                     VRARMPDALFVFLAPPSWEELVRRLTGRGTESEEDRARRLETAVEELAAEREFDVTVV
                     NTDVRTAAQELVSLMRSATDPVDVRDARHTVGTARPPASSSTSRVAPTTE"
     gene            complement(3624..3944)
                     /gene="mihF"
                     /locus_tag="VH419_16630"
     CDS             complement(3624..3944)
                     /gene="mihF"
                     /locus_tag="VH419_16630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007077635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor, actinobacterial type"
                     /protein_id="HEY7045298.1"
                     /translation="MAVPELTAEQRRAALEKAAQVRRQRAEVKNRLKHSGASLTDVLH
                     SGEHDEVVAKMKVLDLLQAVPGLGKVRARNLMERIGISESRRVRGLGANQIAALEREF
                     EHRS"
     gene            complement(4171..4806)
                     /locus_tag="VH419_16635"
     CDS             complement(4171..4806)
                     /locus_tag="VH419_16635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7045299.1"
                     /translation="MDRDVAPDDRSAGPPGSPGSQRPRSPASWVPAAVAVAAVLVLVV
                     GGGVSFALLRGESDPHAASPPSTTSTTSTTSTTSTTSTTGGTTVDESAYCAKLGDIES
                     SLEKYRSSQMDTADLDQTIADFEDLQAVAPHSMQDEIETYLSGFRALRSLLDDLGITF
                     GQFQDPTYLAGHAAEWNAHQQQEIQSVTDQLTQPEFVAAGRALDDDFKERC"
     gene            complement(4810..5373)
                     /locus_tag="VH419_16640"
     CDS             complement(4810..5373)
                     /locus_tag="VH419_16640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7045300.1"
                     /translation="MSQQGPPPWDPDNQPPPRQPGDQPPPWEQSGQPPWQQGGPGGPP
                     RQYPRQHLLPQEVKPHRNRAPLFVGLGVVLVLIVAGVVTYLVLRDSGEDTRQAYCTEL
                     RRLTHDGDLAGALQSADASAEQEIKKVAGLAPDAVADDWDALVPFVEKAAQSGTPDWG
                     GALMAFNALKVIAQDAKDNCGIDMPTL"
     gene            complement(5456..5809)
                     /locus_tag="VH419_16645"
     CDS             complement(5456..5809)
                     /locus_tag="VH419_16645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7045301.1"
                     /translation="MTKRRPVIAVAALLCAGMLTACGGGDGSGDYCNTIKDAVDNDES
                     LTEAEGMDLAKKVRDQAPDEVRDDWDALIDAYNSIGGGDLSDLDPSTLTKMQDAVDNI
                     TKFTKDECDVDLEET"
     gene            complement(6026..6853)
                     /gene="pyrF"
                     /locus_tag="VH419_16650"
     CDS             complement(6026..6853)
                     /gene="pyrF"
                     /locus_tag="VH419_16650"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEY7045302.1"
                     /translation="MEPFGTRFRHAMQTRGPLCVGIDPHPGLIAAWGLDDTPAGLQRF
                     ALTCVEALAAEVAVLKPQSAFFERHGSAGVAVLEQTVAAARAAGALVLLDAKRGDIGS
                     TMQAYADAYLDQTSPMAVDAMTVSPYLGFESLRPVLDTAATAGAGVFVLALTSNPEGP
                     QVQQARGLQGTVAGGVLAAIAAENAGAEPMGSVGAVVAANLTSCVEDLDVNGPLLAPG
                     YGAQGGSAGDVLRIFGGVRANVLPSTSRDVLRHGPDVDSLRDAARRTRDEVSALTAR"
     gene            complement(6857..7873)
                     /locus_tag="VH419_16655"
     CDS             complement(6857..7873)
                     /locus_tag="VH419_16655"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF005574.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEY7045303.1"
                     /translation="MDGATLRPEDVDLSTVLADAHLENPVMTASGCAAAGRELHQFFD
                     VASIGAVVTKSIMRDPRSGRPTPRMAETPSGMLNSIGLQGPGIDGFLARDLPWLLQHD
                     ARPIVSIAGSSLGEYVELARRVGNTPGVAAIEVNISCPNVENRGLVFACDPFQAARVL
                     SAVRRDTPRGVPVFAKLSPDVTSIVEVAKAVVEAGADGLVLINTLLGMHVDLHTMQPH
                     LGGGTGGLSGPAIRPVAVRAVWQVAAAMPGVPIVGVGGIRTGADALEFIVAGASAVQV
                     GTAIFNDPSAPVRVIDELRHELAIRGIGKVSEAIGLAHQPARSIPRAVLDRPHDPDDH
                     RAEG"
     gene            complement(7873..8781)
                     /locus_tag="VH419_16660"
     CDS             complement(7873..8781)
                     /locus_tag="VH419_16660"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HEY7045304.1"
                     /translation="MTPLDTANPAAPTGRGERTPVQVVGKVFSLKRVGDYHHLTLVAD
                     GVAERTRPGNFVALAVGGETSATLLRRAFSIYRVRPAGIYGGTVEIVFAAHGPGTRWL
                     RDVQQHDEVDVVGPLGRPFALPREPVACALIGGGYGSAPLFMLAERLRERRCAVHMVL
                     GAATESRLFGALEAKRSAQSVTVTTVDGSVGIKGLVTEPLPALISRNRVEVVYACGPM
                     GMLQAVAEVAQRHGAWSQTAVEESMACGVGVCMTCVLPVRGDDGVIRMTRSCVDGPVF
                     AGDKVCWEAVGTIPPGTHGAPTGRGH"
     gene            complement(8778..>10578)
                     /gene="carB"
                     /locus_tag="VH419_16665"
     CDS             complement(8778..>10578)
                     /gene="carB"
                     /locus_tag="VH419_16665"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEY7045305.1"
                     /translation="GEDGVRTLRHRLTVRPVYKTVDTCAAEFAAKTPYHYSSYDEETE
                     VGLRTRPAVIILGSGPNRIGQGIEFDYSCVHAAMALSAAGFETVMVNCNPETVSTDYD
                     TSDRLYFEPLTLEDVLEVVAAETVAGPVAGVLVQLGGQTPLGLAEGLAAAGVPIVGTS
                     PEAIHLAEHRGAFGHVLANAGLPAPKHGTATSLSEATQVAHEIGYPVLVRPSYVLGGR
                     GMEIVYGEEELTAALDRLGEGGGERFTHAVLIDRFLDDAIEVDVDALYDGDELFLGGV
                     MEHIEEAGIHSGDSSCVLPPITLGVEDVARIRESTRAIADGVGVRGLINIQFAVAADV
                     LYVLEANPRASRTVPFVSKATATPLAKAAARIMLGASIAELRSEGLLPATGDGGDLPP
                     DAPVAVKEAVMPFNRFRTEDGRNVDTVLGPEMRSTGEVLGLADAFGSAFAKAQTAAFG
                     GLPVKGRAFVSVANPDKRHMIFPIKRLADLGFDILATEGTAAVLRRNGVPATVVRKHS
                     AGPGPGGELTTVGRILAGEVDIVINTPHGSTSGGSPRMDGYEIRTACVAQNIPCITTV
                     QGFGATVQGIEALRANSLGVRSLQSWHSEGGDA"
BASE COUNT         1598 a         3698 c         3633 g         1649 t
ORIGIN      
        1 gatgtctaca cgccgcttgg gctgtaaaca tcgcaaacct caagcggacg aaggcgtcag
       61 gagcggacgg ctgctttcag ctcggcgact cggtcggtgc gctcccaggt gaactcgggc
      121 aggtcacgac cgaagtggcc gtacgccgcg gtcagcgcat agatcgggcg cagcatgtcg
      181 aggtcacgga tgatcgccgc cggacgcagg tcgaagacct ccaagaccgc ggcccggatg
      241 gcgtcgacct cgacggtctc ggtaccgaag cactcgacgt acacgccgac ggggtgggcc
      301 ttcccgatcg cgtacgccac ctgcacctca cagcgccggg cgagacccgc agcgacgacg
      361 ttcttcgcga cccaacgcat ggcgtacgcg ccggaccggt ccaccttcga cgggtccttg
      421 ccgctgaacg cgcctccgcc gtgccgggcc atgccgccgt aggtgtcgat gatgatcttg
      481 cggccggtca ggccggcgtc gcccatcgga ccaccgacct cgaagcggcc cgtggggttg
      541 accagcagcg tgtagtcgct cgcgtcgacg tcgaagtggg acagcacctc gtcgacgaca
      601 tgcttcttga cgtccggggt caacagccgc tcgaggtcga tgtctgccgc gtgctgcgac
      661 gagatcacga cggtgtccac gcggactggg cggtcgccgt cgtactcgat ggtcacctgg
      721 gtcttgccgt ccggccgcag gtacggcatg gtgccgtcct tgcgcacagt ggtcagccgc
      781 tcggccagtc gatgcgcgat cgtgatggga agcggcatca gctcgggcgt gtcgtcgcag
      841 gcgtacccga acatgaggcc ctggtcaccg gcaccctgcc ggtccagcgg gtcggacgag
      901 tggtcgtgac gctcctcgta ggcgtcatcg acaccctgcg cgatgtcctt cgactgcttg
      961 tcgatcgcga cctgtacgcc gcacgacgcg ccgtcgaagc ccttcttgga cgagtcgtac
     1021 ccgatgtcga ggatgcggtc gcggacgatg tcggcgatcg gggcgtacgc ctcggtggtg
     1081 acttcaccgg caacgacgac caatcccgtc gtcaccaacg tctcgacagc gacccggctt
     1141 cggggatcct gctcgagcag agcgtcgagg atggagtcgc tgatctggtc ggcgatcttg
     1201 tcgggatggc cttcggtgac ggattccgac gtgaacagtc gtccggtcaa gcttctctcc
     1261 tgtgtcaggg tccgagtcca cggacctgcg gcgggtcgca atcagggtag accagagcgg
     1321 cgtcgatcaa gcgagcggtc cagtgcgtgt ccgtatctgc gccgtccggc gctcacatcg
     1381 cgagccgctc gaccacacag tcccagacga cgtgtgccag cgcggccttc gagcccctcg
     1441 ggacggctcg ttgcgaccct tctcggccca ggatcacggc ctggttgtcg tcttcgccga
     1501 acacggcgcc ggcgctgacg tcgttgacga ccagcaggtc acagcccttg cgggccagct
     1561 tcctgcgcgc gtggtcgagc accgagccgg tcgcgtcgcc ggtctcggcg gcgaagccga
     1621 cgaggacctg gctcgtggat cggctccgac gtgccacgag atcggccaga atgtccgggt
     1681 tctccaccag cgggacgtct ggggtcacac cgtccggctg tttcttgatc ttgtgcgtga
     1741 cccggacggc cggccggaag tcggcgggcg cggccgccat cacgatcacg tcggccgccg
     1801 ccgcagccgc caccatcgcc tcctgcaggt cgagtgcgga cacgacctgt acgaccttgg
     1861 cgcctgcggg atcgggcagg tcgacgttcg ccgccaccaa ggtcacgtcg gcgccgcgcg
     1921 ccacggccgc cctggccaag gcgtacccct gcttgccgga gctccggttg ccgaggaacc
     1981 gcaccgggtc gaggtattca cgggtgccgc cggcgctcac cagcacccgc cgcccacgga
     2041 ggtcctgctc gaggccggcc tggccacggt ggagcatgtc ggtggcgacc gagaagatct
     2101 cgtccggttc gggcagccgc ccaggaccgg tgtccttccc ggtgagccgg ccgtacgccg
     2161 gctcgatcac cagcaccccg cgcccacgca gcgtcgcgac gttcgcctgg gtggccggat
     2221 gctcccacat ctccgtgtgc atggccgggg ccatcaccac cgggcaccgt gccgtgagca
     2281 gggtcgcggc gagcaggtcg tcggcccggc cgtgcacggc gcgggccagc aggtcggcgg
     2341 tggctggagc cacgacgaca aggtctgctg tctgaccgag gcgcacgtgc tggacggtat
     2401 cgacgttctc gaagacgcct gacgccaccg gatgaccgga gagcgcctcc caggtggccg
     2461 cgccgacgaa ccgtaacgcc gactcggtcg ggacgacggt gacgtcgtgg ccggactcgg
     2521 tgaaccgccg aagcagctcg cacgccttgt acgcagcgat gccgccactc acgccgagca
     2581 ggacgcgagg gcggctctgg tcgctcatgt cggtggtggc gtgaccgtgt gccggtgggc
     2641 ggtcaggcct cgtcagactc cggctcgaca tcctcacacg tcagcaggtc gctgtcgatc
     2701 tcgcgcagcg cgatcgacaa cggcttctcc tggacgtggg tgtcgacgag cgggccgacg
     2761 tactccagca ggccttcgcc gagctgggag tagtaggcgt tgatctggcg cgcacgcttc
     2821 gcggagtaca gcacgagctt gtacttcgag tcggtcttct cgagcaggga gtcgatcgga
     2881 gggtgggtga tcccctcggc gacgggcagg gagccggaca cggcaggaag cctcactcag
     2941 tggttggggc gacccgagac gtcgacgacg aagccggtgg cctcgcagtc ccgacggtgt
     3001 gacgagcgtc acgtacgtcg accgggtcgg ttgctgaacg catcaaggat accaattcct
     3061 gcgccgcggt acgaacgtcg gtgttcacga cggtcacgtc gaactcgcgc tcggccgcga
     3121 gctcttcgac agccgtctcc aaccgtcgcg cccggtcctc ctccgactcc gtgccgcgcc
     3181 cggtcaaccg gcgaacgagc tcctcccacg acggcggcgc aaggaagacg aacaacgcgt
     3241 cgggcatcct cgcccgcacc tgccgagctc cttgcaggtc gatctcgagc agcgccagcc
     3301 gtccgtcgcg gaccgccttc tcgaccggcg cgcgcggcgt gccgtaccgc gccgccttgt
     3361 gcaccaccgc ccactccagg agctcgtcgc gctcgatcat cgcgtcgaac tccgcgtcgt
     3421 cgacgaatcg atagtggacg ccgtcgacct cacccggacg ggccggccgg gtggtgacgg
     3481 agaccgagat ccagatgtcg gagtactcgg cgcgaatctg tgcggacacc gttcccttgc
     3541 cgaccgcggt cggccccgca agcacggtga gacgagaacg ccgctccggt acgccgcgct
     3601 ccgcggctga ccgctcgggc gcgtcaggaa cggtgctcaa actcgcgctc gagggcggcg
     3661 atctggttgg cgccgagacc gcgcacgcga cgtgactcgg agatcccgat ccgctccatc
     3721 aggttgcgtg cacggacctt gccgagtccc ggcaccgcct ggagcaggtc gaggaccttc
     3781 atcttcgcca cgacctcgtc gtgctcgcct gagtggagca catcggtgag cgaggcaccg
     3841 gagtgcttga gacggttctt cacctcggct cgttgccggc ggacctgcgc tgccttctcc
     3901 aacgcggctc ggcgttgctc ggcggtcaac tccggcacag ccacggctcc cacctctgct
     3961 cgatcgtcgc tcgaccgttg cgcgtcagct gcccgtcatg tgctcgacgg tgcgcgacag
     4021 atgagaactc ctcgatccgg cacgacgacc ccgctggcga tgatgctgac gacgctgatg
     4081 ccaacgtagc gcgacgacca ctgctgtgca caaggggacg gacggcgttc ggcaacagtc
     4141 gcgcacgtgt tcagatgcat gtctggcgta tcagcaacgc tccttgaagt cgtcgtcgag
     4201 tgcgcgtccc gcagcgacga actccggctg ggtgagctgg tccgtcacgc tctgtatctc
     4261 ctgctgctgg tgggcgttcc actccgccgc gtgaccggcc aggtacgtcg ggtcctggaa
     4321 ctggccgaag gtgatgccga ggtcgtcgag caacgatcgc agggcgcgga aaccggagag
     4381 gtacgtctcg atctcgtcct gcatcgagtg cggggccacg gcctggagat cttcgaagtc
     4441 cgcgatcgtc tggtcgaggt cggcggtgtc catctgggac gatcgatact tctccagcga
     4501 tgactcgatg tcgccgagct tcgcgcagta cgccgactcg tcgaccgtcg taccgcccgt
     4561 ggtggacgtg gtggacgtcg tggacgtggt ggacgtggtg gacgtggtgg agggagggct
     4621 ggcagcgtgc ggatcggact caccacgcag cagcgcgaac gagacacccc cgccgacgac
     4681 gagcacgagc acggccgcca ccgcgacggc ggccgggacc cacgatgccg gcgatcgtgg
     4741 acgctgcgac cccggtgagc ctggcggtcc cgccgagcgg tcgtcgggtg cgacgtcgcg
     4801 gtccatggcc tacagcgtgg gcatgtcgat gccgcagttg tccttggcgt cctgcgcgat
     4861 caccttgagc gcgttgaacg ccatgagggc gccaccccag tcgggggttc cggactgggc
     4921 ggccttctcg acgaagggca ccagtgcgtc ccagtcgtcc gcgaccgcgt cgggtgcgag
     4981 cccggcgacc ttcttgatct cctgctccgc cgacgcgtcc gcgctctgca aggccccggc
     5041 caggtcgccg tcgtgggtga gccggcgcag ctccgtgcag tacgcctgcc gagtgtcctc
     5101 gccgctgtcg cgcagaacca ggtaggtcac gaccccggcg acgatgagca cgaggacgac
     5161 gccgagcccg acgaacagcg gcgcccggtt gcggtgcggc ttgacctcct gcggaagcag
     5221 gtgttgtcgc gggtactggc gcggcgggcc ccccgggccg ccctgctgcc acggaggttg
     5281 gccgctctgc tcccacggcg gcggctggtc gccgggctgc cgcggtggcg gctggttgtc
     5341 cggatcccag ggtggcggcc cctgctgtga catgcgtctg ctcccattcc agccggtcga
     5401 cggctccagt ctgccaaggc gaccaagtcc ggcggccgat cgacgaaccg cgcgatcagg
     5461 tctcctcgag gtcgacgtcg cactcgtcct tggtgaactt cgtgatgttg tccacggcgt
     5521 cctgcatctt cgtcagcgtc gacgggtcga gatcggacag gtcgcctccg ccgatcgagt
     5581 tgtaggcgtc gatgagcgcg tcccagtcgt cgcggacctc gtccggcgcc tggtcgcgaa
     5641 ccttcttcgc caggtccatc ccctcggctt cggtgagcga ctcgtcgttg tccactgcgt
     5701 ccttgatcgt gttgcagtag tcaccggacc cgtctccgcc accgcatgcg gtgagcatgc
     5761 ccgcgcagag cagtgcggcc accgcgatga ccggtcgtcg tttcgtcatg gtcttctccc
     5821 cttgtcgtcg aggcgacgcg tgcgcctcag tgtgctgtgc caacagacga cggcggctgt
     5881 cggagccgag ggccagcgac acacccttgc acgtcacggt gtcactcgcg ccaccggatc
     5941 gccgcggcgg gggcccccga attgcgggaa agttgctgcc gacacggggc tccaggccac
     6001 acgtttcccg caattcgcgg gagcctcagc gggcggtgag cgccgagacc tcgtcgcggg
     6061 tacgacgtgc agcgtcacgc agggagtcca cgtcaggacc gtgacggagt acgtcgcgtg
     6121 acgtgctcgg cagcacgttc gcacggacac cgccgaagat ccggagcacg tcaccggcgc
     6181 tgccgccctg ggctccgtaa ccgggcgcca gcaggggtcc gttgacgtcg aggtcctcca
     6241 cgcaggacgt cagattcgcg gccacgacag cacccaccga gcccatcggc tcggcgccgg
     6301 cgttctcggc ggcgatcgcg gccagcaccc caccggcgac cgtgccctgc aggccgcgtg
     6361 cctgctgcac ctgcggaccc tccgggttcg acgtgagcgc cagcacgaac acacccgcac
     6421 cggcggtcgc agccgtgtcg aggaccggcc gcagcgactc gaagccgagg taggggctca
     6481 ccgtcatcgc atcgaccgcc atgggagagg tctggtcgag gtacgcatcg gcgtacgcct
     6541 gcatggtcga tccgatgtcg ccacgtttgg cgtcgagcag cacgagcgcc ccggcagcgc
     6601 gggccgccgc cacggtctgc tccagcacgg cgacaccagc ggagccatgc cgttcgaaga
     6661 acgccgactg cggcttcagc accgcgacct cggccgccaa cgcctccacg caggtgagcg
     6721 cgaaccgctg gagccccgcc ggagtgtcgt cgagtcccca cgccgcgatc agaccgggat
     6781 gcgggtcgat gccgacacag agtgggccac gcgtctgcat cgcgtgacga aaccgagtac
     6841 cgaatggctc cacgaatcat ccttccgcac ggtggtcgtc ggggtcgtgt gggcggtcga
     6901 gcacggcccg cgggatcgat cgggccggtt ggtgcgcgag tccgatcgct tcgctcacct
     6961 tgccgatacc acggatcgcc agctcgtgac gcagctcgtc gatgacgcgc accggcgcgc
     7021 tcgggtcgtt gaagatggcg gtccccacct ggaccgccga cgcgccggcg acgatgaact
     7081 ccagcgcgtc cgccccggta cggataccgc cgacgcccac gatcggcaca ccgggcatgg
     7141 ccgcagccac ctgccacacc gcccggacgg ccaccggtcg gatcgcgggc ccggacaggc
     7201 ctcccgtgcc gccgccgagg tggggttgca tcgtgtggag gtcgacgtgc atgccgagca
     7261 gcgtgttgat gaggacgagc ccgtcggcgc ccgcctccac cacagccttc gcgacctcca
     7321 cgatgctggt gacgtcaggc gacagcttgg cgaagaccgg tacgccgcgt ggcgtgtccc
     7381 gtcgtaccgc ggagagcacc cgcgcggcct ggaacgggtc gcacgcgaag acgagcccgc
     7441 gattctcgac gttcgggcac gagatgttga cctcgatcgc cgcaacgccg ggtgtgttgc
     7501 cgacccgccg ggccagctcg acgtactccc ccagcgacga gccggcgatc gagacgatcg
     7561 gtcgggcgtc gtgctggaga agccagggca ggtcacgggc caggaacccg tcgatcccgg
     7621 gtccctggag accgatggag ttgagcatgc cggacggcgt ctccgccatc cgcggcgtgg
     7681 gtcgtccgga ccgtgggtcg cgcatgatgg acttggtgac gaccgctccg atcgaggcga
     7741 cgtcgaagaa ctggtggagc tcccgcccgg ccgcagcgca cccgctggcg gtcatcaccg
     7801 gattctcgag atgtgcgtcg gcgagcacgg tggacaggtc gacgtcttcg ggtcgaagtg
     7861 tggcgccatc catcagtggc cccgtcccgt cggcgcgccg tgggttcccg gcggtatcgt
     7921 cccgaccgcc tcccagcaga ccttgtcgcc ggcgaacacc ggcccgtcga cgcaggaccg
     7981 ggtcatccgg atcacgccgt cgtccccgcg cacgggcagc acgcaggtca tgcagacacc
     8041 gacaccgcag gccatcgact cctcgacagc cgtctggctc cacgccccgt gcctctgggc
     8101 gacctcggcc accgcctgca gcatgcccat cgggccgcac gcgtacacga cctcgacccg
     8161 gttgcgcgag atgagcgccg gcagcggctc ggtcaccaga cccttgatgc cgacggaccc
     8221 gtcgaccgtc gtgaccgtga cggactgggc ggagcgtttg gcttcgagcg ctccgaacag
     8281 gcgtgactcg gtggctgcgc cgagcaccat gtgcaccgca cagcgtcgct cccgtagccg
     8341 ctcggccaac atgaacagcg gtgccgagcc gtagccaccg ccgatcagcg cgcaggccac
     8401 cggttcgcgg ggcagggcga agggacggcc gagcgggccg accacatcga cctcgtcgtg
     8461 ctgctgcacg tcccgcagcc agcgggtgcc cgggccgtgc gcggcaaaca cgatctcgac
     8521 cgtcccgccg tagatgccgg ccggccggac gcggtagatc gagaacgccc ggcgcagcaa
     8581 ggtggccgac gtctcgccac cgacggcgag cgccacgaag ttaccggggc gtgtccgctc
     8641 tgcgacaccg tcggcgacga gcgtcagatg gtggtagtca ccgacgcgct tgagcgagaa
     8701 gaccttcccg accacctgca ccggcgttcg ctcgcctcgt cctgtggggg cggctgggtt
     8761 ggccgtgtcg aggggtgtca cgcgtctcct ccctcggagt gccagctctg caaggaccgt
     8821 acgccgagac tgttcgcgcg cagcgcctcg atcccctgca cggtggcccc gaacccctgc
     8881 acggtcgtga tgcacgggat gttctgcgcc acgcaggcgg tacggatctc gtagccatcc
     8941 atccgcgggg aaccgcctga cgtcgaaccg tgcggcgtgt tgatgacgat gtcgacctcg
     9001 ccggcaagaa tgcgcccgac ggtcgtgagc tcgccgccgg gacctggtcc ggccgagtgc
     9061 ttgcgaacga ccgtcgccgg gacgccgttg cgtcgcagca cggccgcggt cccttcggtg
     9121 gccaggatgt cgaagccgag atcggccaac cgcttgatcg ggaagatcat gtggcgcttg
     9181 tcggggttgg cgaccgacac gaaggcacga cccttgaccg gcaggccgcc gaacgctgca
     9241 gtctgcgcct tggcgaaggc ggaaccgaag gcgtccgcga gtccgagcac ctcccccgtc
     9301 gatcgcatct cggggccaag caccgtgtcg acgttacggc cgtcctcggt acggaagcgg
     9361 ttgaacggca tcaccgcctc cttcaccgcg accggcgcgt ccggcggcag gtcccccccg
     9421 tcaccggtgg cggggagcag cccttcgctc cgcagctcgg cgatcgacgc gccgagcatg
     9481 atccgggcag ccgccttcgc caacggggtg gccgtggcct tcgagacgaa cggcacggtc
     9541 cgcgacgccc tcgggttggc ctcgaggacg tacagcacgt ccgcagccac ggcgaactgg
     9601 atgttgatga gcccccggac tcctacaccg tcggcgatcg cgcgggtcga ttcgcggatc
     9661 cgggccacgt cctcgacgcc caacgtgatc ggcggcagga cgcaggagga gtccccggag
     9721 tggatcccgg cctcttcgat gtgctccatg acgccgccga ggaagagctc gtcgccgtcg
     9781 tacagcgcgt cgacgtcgac ctcgatcgcg tcgtcgagga agcggtcgat caggaccgcg
     9841 tgggtgaacc gctcgccgcc gccctcaccg agccggtcca acgcggcggt cagctcctcc
     9901 tcgccgtaga cgatctccat gccacgacct ccgaggacgt acgacggtcg cacgagtacg
     9961 gggtagccga tctcgtgtgc gacctgggtg gcttcggaga gcgacgtcgc ggtcccgtgc
    10021 ttcggcgcag gcaggccggc gttcgccagc acatgtccga aagcgccacg gtgctcggcg
    10081 aggtgaatgg cctccgggct cgtaccgacg atcgggacac cggcggccgc caggccttcc
    10141 gcgaggccga gcggtgtctg accgccgagc tgcaccagga cgccggcgac aggtccggcg
    10201 acggtctcgg cggcgacgac ctcaaggacg tcctcgagcg tgagcggttc gaagtacaac
    10261 cggtccgagg tgtcgtagtc ggtcgacacc gtctccgggt tgcagttgac catcaccgtc
    10321 tcgaagccgg ctgccgacag cgccatcgcg gcatggacac aggagtagtc gaactcgatg
    10381 ccctgcccga tccggttggg tccgctgccg aggatgatca ccgcgggccg ggtgcgcagt
    10441 cccacctcgg tctcctcgtc gtacgacgag tagtggtacg gcgtcttggc cgcgaactcc
    10501 gccgcgcagg tgtcgacggt cttgtagacc ggacgcacgg tcagccggtg ccgcagcgtg
    10561 cggacgccgt cctcaccg
//
LOCUS       DATBFZ010000546         2735 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_3895057, whole genome shotgun sequence.
ACCESSION   DATBFZ010000546 DATBFZ010000000
VERSION     DATBFZ010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2735
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3895057"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1018
                     /locus_tag="VH419_16670"
     CDS             <1..1018
                     /locus_tag="VH419_16670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020387549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEY7045306.1"
                     /translation="VVAGWLFGALIAFVISTVFGMVGADRDAPAERALSSMPMTYRTM
                     AIVLNPIKVPDSEGFKARMGSAARAAGWDEPLWFETTVDDAGASMAKAAVAAGADVVV
                     AAGGDGTVRVICAEMAGTGVPVGVIPAGTGNLLARNLGVPLAHDEAIDTVLRGQDRAI
                     DVVSIDGDNLDTTRFVVMAGLGLDAAIMAGAPDALKARIGWTAYVVAGARHLRYPAVR
                     VDISVDGAEPVRRRARTVVIGNVGSLQAGIPLLPDALIDDGLLDVVVVAPRRLFGWVP
                     VALRVFTRGRRIDERLDRYTGRSVSLTVASPAPRQLDGDTVPPGTHLHATVEPGLLLV
                     RVPR"
     gene            1158..1619
                     /locus_tag="VH419_16675"
     CDS             1158..1619
                     /locus_tag="VH419_16675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008355933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4446 family protein"
                     /protein_id="HEY7045307.1"
                     /translation="MPLSATLLSWLTLVALGLAAVGVVIALAAWRRAGVRRFGGRRPI
                     KRRMLPRPRTKTLPLTSEEVSAEIHRLRRDVASTLRHMAVVRYDAFGDMGGHLSWSMA
                     LLDDGGDGVVLTSIHGRSDSRTYAKNVSAWSANQQLSPEEEEAIGFARAEA"
     gene            complement(1738..2670)
                     /gene="pheA"
                     /locus_tag="VH419_16680"
     CDS             complement(1738..2670)
                     /gene="pheA"
                     /locus_tag="VH419_16680"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020579823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEY7045308.1"
                     /translation="MPGVPPARYAYLGPEGTFTEAALRSLPAAARAELQPLPTITAAL
                     DAVRVSEADGAVVPMENSVEGGVAVTIDELSTGDPLMITREVNIEVQFALLAKHGTRP
                     EAIKKVATHPHAEAQCRRWLATHLPDAEVVLESSTAHAAAMVAVTNSPYDAAISAPIA
                     AQTYRLATLANGIADRAGAVTRFVLVSRPGTPPAPTGADKTSVVAFIQDNHPGALLEL
                     LEQFAARGVDLTRIESRPIGSALGKYCFSIDAEGHVADARMGEALMGLRRTCDEVRFL
                     GSYPRADGTPTSVSAGTSDGDFADAAAWLTRLRS"
BASE COUNT          381 a          951 c          965 g          438 t
ORIGIN      
        1 cgtggtcgcc gggtggctgt tcggcgcgct catcgccttc gtcatctcca cggtcttcgg
       61 gatggtcggg gcggaccgcg acgcgcccgc cgagcgtgcg ctgtcgtcga tgccgatgac
      121 ctatcgaacg atggcgatcg tcctgaaccc gatcaaggtc ccggactccg aaggattcaa
      181 ggcgcggatg ggatccgctg cccgtgcggc gggttgggac gaaccgctgt ggttcgagac
      241 gacggtcgac gacgccggtg cctcgatggc gaaggctgct gtcgctgcgg gtgccgacgt
      301 cgtggtcgcc gctgggggtg acggcacggt tcgtgtcatc tgcgcggaga tggcggggac
      361 gggggtaccg gtcggggtga tccctgccgg aacggggaac ctgctggccc gcaacctcgg
      421 cgtcccgctc gcccacgacg aggccatcga cacggtgttg cgcggccagg accgcgcgat
      481 cgacgtcgtc tcgatcgacg gggacaacct cgacacgacc cgcttcgtcg tcatggccgg
      541 gctcggcctc gacgctgcga tcatggccgg tgcgccagac gcgctgaagg cgcgcatcgg
      601 ctggacggcg tacgtcgtgg ccggcgcccg gcacctgcga tacccggcgg tccgcgtcga
      661 catcagtgtc gacggcgctg aaccggtacg ccgtcgggcc cggactgtgg tgatcggcaa
      721 cgtcgggtcg ctgcaggccg gcatcccact gctgcccgac gcgctgatcg acgacgggct
      781 gctcgacgtg gtggtggtcg cgccgcggcg gctgttcggc tgggtgccgg tggcgcttcg
      841 ggtcttcacc cggggccggc gcatcgacga gcggctggac cgctacaccg gccgatcggt
      901 ctccctgacc gtggccagtc cggcgccccg ccagctcgac ggcgacaccg tacctcccgg
      961 gacccacctg cacgccaccg tcgaacccgg cctcctcctc gtccgggtcc cccgctgacg
     1021 ccgacacttt tcgccgaggt ggcaggtttc gttggagttt gtcggcgtgt cgcacatgga
     1081 ttgccacctc gatgatggac tgacgcgtcc acggcgggtc atcctggtcg cggtccgccg
     1141 tcgacttacg ctcgggtatg ccgttgtccg cgaccctcct gagctggctc acgctggtgg
     1201 cactcggact cgctgcggtc ggcgtcgtga tcgccctcgc cgcctggcgc cgagccggcg
     1261 tacgacgatt cggcggtcgg cgtcccatca agcgacggat gcttccgcgc cccaggacca
     1321 agacgctccc gctgacctcg gaggaggtct ccgctgagat ccatcgcctc cggcgtgatg
     1381 tcgccagcac gttgcgccac atggcagtgg tccggtacga cgcgttcggt gacatgggcg
     1441 gtcatctgtc ctggtcgatg gcactgctcg acgacggcgg tgacggcgtt gtcctgacgt
     1501 cgatccacgg tcgcagtgac tcgcgcacct atgccaagaa cgtcagcgca tggtccgcca
     1561 accagcaact gtcgccggag gaggaagagg cgatcggctt cgcccgcgcc gaggcctgag
     1621 ccaacggatc gaggtcacgg gcttcgcgtc gcgatccggc acacgccgtt caccgaggtg
     1681 gcaaccgttg cgcgacacgc cgtcaaactc caacgaaacc tgccacctcg gcgggggtca
     1741 ggagcggagg cgggtgagcc aggcggcggc gtcggcgaag tcgccgtccg acgtacctgc
     1801 cgagaccgag gtcggagtcc cgtccgcgcg tgggtaggag ccgaggaagc ggacctcgtc
     1861 gcacgtacgc cgcagaccca tcagcgcctc acccatccgc gcgtccgcga cgtggccttc
     1921 ggcgtcgatc gagaagcagt acttcccgag cgcagagccg atgggacgcg actcgatcct
     1981 cgtcaggtcc accccgcgcg ctgcgaactg ctcgagcagc tccaacaacg cgcccgggtg
     2041 gttgtcctgg atgaacgcca cgaccgacgt cttgtccgcc ccggtcggag ccggcggcgt
     2101 gcccggtctg gacaccagca cgaaccgcgt caccgcaccg gcccggtccg cgatcccgtt
     2161 ggcgagtgtg gccaggcgat acgtctgcgc ggcgatgggt gcggagatgg cggcgtcgta
     2221 cggcgagttc gtcaccgcca ccatcgcggc ggcatgtgcg gtcgacgact cgagcaccac
     2281 ctcggcgtcc ggcaggtgcg tcgccagcca gcgccggcac tgcgcctcgg cgtgcgggtg
     2341 cgtggccacc ttcttgatcg cctccggccg ggtgccgtgc ttggccagca gcgcgaactg
     2401 gacctcgatg ttcacctcgc gcgtgatcat cagtgggtcg ccggtcgaga gctcgtcgat
     2461 ggtcacggcc acaccacctt cgacggagtt ctccatcggg accaccgcgc catctgcctc
     2521 ggacacgcgt acggcgtcca gcgccgccgt gatcgtgggc agcggttgca gctccgcccg
     2581 cgccgcggcc ggcaacgacc gcagcgccgc ctcggtgaag gtgccttccg gaccgaggta
     2641 cgcgtagcga gcgggcggga ctccgggcat gggaaccact gtagggaggc tagtgccgcg
     2701 tcaaccaacg ttgctcgtcg tgagcggtgt ccggg
//
LOCUS       DATBFZ010000547         2172 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_4896763, whole genome shotgun sequence.
ACCESSION   DATBFZ010000547 DATBFZ010000000
VERSION     DATBFZ010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4896763"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="VH419_16685"
     CDS             <1..323
                     /locus_tag="VH419_16685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008356461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY7045309.1"
                     /translation="EAEEAAASLGAGRFTVFRRIIVPGLAPAIAAGGALSFARAVSEY
                     GSLVLLSGNLPNRTEVTSVRIITFIEGDRLDNAAAVAVVLLLISLVVIVAVDLLQRSV
                     VRRG"
     gene            316..1242
                     /locus_tag="VH419_16690"
     CDS             316..1242
                     /locus_tag="VH419_16690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007078505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit"
                     /protein_id="HEY7045310.1"
                     /translation="MAEPSIIHKGRPRPGAARASKRSPGTYVLRVLVIAYLFFLVAWP
                     VYLVGRNAFARGLTDLGAILSDPYTVYSLELTLWIAVASVAINTVFGVGISLLIVRFR
                     FVGRRVLNALLDLPLSVSPVVVGLALVLVYGGRTGWFGPTVQRWGLTVIYDVPGMILA
                     TTFVSLPLVIREVIPVLQEVGDEQEQAARTIGANGLQTFFRVTLPAIRWGVSYGVVLC
                     LARSLGEFGAVKVVSGNIYGETRTATLTVENEYLNFDKGGAYATAFLLTLVAVAALLV
                     ASLARNAPSRARSAPSRGRDVTEQPRQPEERR"
     gene            1239..>2172
                     /locus_tag="VH419_16695"
     CDS             1239..>2172
                     /locus_tag="VH419_16695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE-like domain-containing protein"
                     /protein_id="HEY7045311.1"
                     /translation="MSIEISHVSKRFDGFVAVDDVCVSIPTGQLTALLGPSGGGKSTL
                     LRIIAGLDRPDSGRIEIAGVDATNLPPQKRNVGFVFQHYAAFKHLTVRRNVAFGLEIR
                     HRPKAEIRARVDELLALVQLEQFADRLPAQLSGGQRQRMALARALAVEPSVLLLDEPF
                     GALDAQVRRQLRDWLRRLHDEVPVTTVLVTHDQEEALEVADEIAVINQGRVEQVGTPD
                     ELYDRPVNDFVMRFLGPVTELGPRLVRPHDLRLTIGTAGGVVTGRVSRIVRVGFEVRV
                     DVATDDQVITVTLPRGEFLELGLRLDTVVAVEEVA"
BASE COUNT          298 a          763 c          741 g          370 t
ORIGIN      
        1 gcgaggcgga ggaggctgcc gcgtcgctgg gagcgggacg gttcaccgtg ttccggcgga
       61 tcatcgtgcc cggtctggcg ccggccatcg cggcgggcgg tgccctctcg ttcgcccgag
      121 cggtcagcga gtacggctcg ctcgtcctgc tctccggcaa cctgccgaac cggaccgagg
      181 tgacgtcggt gcgcatcatc acgttcatcg agggcgaccg tctcgacaac gccgcggccg
      241 tcgcggtcgt cctgctgctc atctcgctgg tggtcatcgt cgccgtggat ctgctgcagc
      301 gcagcgtggt tcggcgtggc tgagccatcc atcatccaca agggacgccc ccggcccggc
      361 gcggccagag cgtccaagcg cagtccgggt acctacgtgt tgcgggtgct ggtcatcgcc
      421 tacctgttct tcctggtggc gtggccggtt tacctggtcg gcaggaacgc gttcgcccgt
      481 ggcctgaccg acctcggcgc catcctgtcc gacccctaca cggtctactc cctggagctg
      541 accctgtgga tcgcggtcgc ctcggtcgcg atcaacaccg tgttcggggt gggcatctcg
      601 ctgctgatcg tacggttccg gttcgtcggc aggcgggtcc tcaacgcact gctggacctg
      661 ccgctgtcgg tctcgcccgt ggtcgtgggt ctggccctgg tgctcgtcta cggcgggcgt
      721 accggatggt tcggccccac cgtgcaacgc tgggggctga cggtcatcta tgacgtgccc
      781 ggcatgatcc tggccacgac gttcgtctcc ctacccctgg tcatccggga ggtcatcccg
      841 gtgctgcagg aggtgggcga cgagcaggag caggcggcgc gcacgatcgg cgccaacggc
      901 ctgcagacgt tcttccgggt gacgctgccc gccatcaggt ggggcgtcag ctacggcgtg
      961 gtgctgtgcc tcgcccgatc actgggcgag ttcggcgcgg tgaaggtcgt ctctggaaac
     1021 atctacggtg agacgaggac ggccacactc accgtcgaaa atgaatatct caatttcgac
     1081 aagggtggtg cctacgccac ggcgttcctg ctcacgctgg tcgccgtcgc cgccctgctg
     1141 gtcgccagcc tcgcccggaa cgcgccgtcg cgcgcccgga gcgcaccctc gcgcggccgt
     1201 gacgtaacgg agcagcccag gcaacccgag gagcggcgtt gagcatcgag atcagccacg
     1261 tcagcaagcg cttcgacggc ttcgtcgccg tcgatgacgt ctgtgtcagc atccccaccg
     1321 gtcagctcac ggccctgctc ggcccctccg gcggtggcaa gtcgaccctg ctgcgcatca
     1381 tcgcgggcct ggatcggccc gactcgggac ggatcgagat cgcgggcgtc gacgcgacca
     1441 acctgccacc gcagaagcgc aacgtcggct tcgtcttcca gcattacgcg gccttcaagc
     1501 acctcaccgt gcgccgcaac gtcgcgttcg gactggagat caggcatcgg cccaaggcgg
     1561 agatccgcgc ccgggtcgac gagctgctcg cgctggtcca actggagcag ttcgccgacc
     1621 ggctgcccgc ccagctctcc ggcggccagc gccagcggat ggccctggcc cgcgcgctcg
     1681 cggtcgagcc gtcggtactg ctcctcgacg aacccttcgg cgcgctggat gcccaggtac
     1741 gcaggcagtt gcgggactgg ctgcgccgcc tgcacgacga ggtgccggtg acgaccgtcc
     1801 tcgtgacgca cgaccaggag gaggcgctcg aggtcgccga cgagatagcc gtcatcaacc
     1861 agggccgcgt cgagcaggtc ggcacacccg acgagctcta tgaccgaccc gtcaacgact
     1921 tcgtcatgcg cttcctcggc ccggtcaccg agcttggccc gcgcctggtc cgcccccacg
     1981 acctgcggct gacgatcggt acggccggtg gggtcgtgac cggacgggtc agccggatcg
     2041 tacgggtcgg tttcgaggtg cgcgtggacg tcgccaccga cgaccaggtc atcacggtga
     2101 ccctgccccg cggcgagttc ctcgagctcg gcctgcggct cgacaccgtg gtcgcggtcg
     2161 aggaggtcgc gg
//
LOCUS       DATBFZ010000548         3585 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_4897381, whole genome shotgun sequence.
ACCESSION   DATBFZ010000548 DATBFZ010000000
VERSION     DATBFZ010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 3585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3585
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4897381"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(169..1071)
                     /gene="thrB"
                     /locus_tag="VH419_16700"
     CDS             complement(169..1071)
                     /gene="thrB"
                     /locus_tag="VH419_16700"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008362126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEY7045312.1"
                     /translation="MPATSANLGPGFDAFGLCLGLYDDLAATATETGLVVDVAGEGAE
                     SVPLDEQHLVIRAMRATFDLLGVAQPGLSLAAENRIPHARGLGSSAAAIVAGIRLAQA
                     LAPDVELGADDALSLAVELEGHPDNVAACLFGGLTIAWTDLQGSSAARVEVDARVRAT
                     VLVPGAGVSTEVARGILPETVPHRAASANAGRAGLLVAALTGQPELLHPATRDWLHQK
                     YRRPAMPATLQLVTALRDSGVAAMVSGAGPSVLVLGTADSPAYDALVPVGWDTLALAI
                     DGVGCVWTPVGAGSGPAEQRSAPT"
     gene            complement(1118..2215)
                     /gene="thrC"
                     /locus_tag="VH419_16705"
     CDS             complement(1118..2215)
                     /gene="thrC"
                     /locus_tag="VH419_16705"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017935496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEY7045313.1"
                     /translation="MTLHASQSAATHQWRGVIEEYRRWLPVSDETPVVTLREGGTPLV
                     QSGWLSERTACSVWLKVEANNPTGSFKDRGMTVAVSNALEQGAKAVVCASTGNTSASM
                     AAYASRAGMTPIVLVPQGKIARGKMAQAIMHAAKIIQVRGGFDDCLDLARTLSKDYPV
                     ALVNSVNPVRLEGQKTAAFEIVDFLADAPEIHVLPVGNAGNIAAYWKGYVEYAATGRA
                     SRRPAMWGFQAAGAAPIVTGHVVPKPETVATAIRVGNPASWLLAEAARDESGGIIEAV
                     SDAQILAAQRTMAARDGVFVEPASAAGVAGLLKRHDDGLLDPGTVVAITVTGHGLKDI
                     DTALEYVGTPEELVVDKDAAQAARAAGLVDG"
     gene            complement(2212..3522)
                     /locus_tag="VH419_16710"
     CDS             complement(2212..3522)
                     /locus_tag="VH419_16710"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEY7045314.1"
                     /translation="MNEPVRIAMLGCGVVGTEVARLLLEQTDDLVARVGAPLELVGIA
                     VRRPDRARAADLPRGLFTTDAHALVTRGDLDVVIEVIGGIEPARSLILAALEAGSSVV
                     TANKALLAEDGSTLFETADKVGRDVYFEAAVAGAIPIIRPLRESLAGDKVRRVLGIVN
                     GTTNYILDQMDTSGASFDDALEQAQSLGYAEADPTADVDGFDAAAKAAILASLAFHTR
                     VTAADVHREGIGDVSAQDVTSARDMGSVVKLLAICDRDGAIESGRPDTVSVRVHPAMI
                     PRAHPLASVREAYNAVYVETEAAGQLMFYGPGAGGGPTASAVLGDVVTVARNRLAAVT
                     GAGESTYAKCEIASMGETITRYHVAIDVDDKPGVLAAVATLFARHQVSIQAVRQEGRG
                     DDAQLVVVTHSAPDAALSATVDGLRSLDVVRDVSSVMRVEGAPE"
BASE COUNT          557 a         1310 c         1218 g          500 t
ORIGIN      
        1 ccaacgcgac catcgccgag cacggccggc caacgcgacc atcgccgagc acggccggcc
       61 aacgcgacca tggcggagca ggcccgccca ccgaagcccg aggagcatcc agatcgaacc
      121 gtgcgacgag cgctccttgg actgaaccgt caacggggag tcgatcggct acgtcggcgc
      181 ggagcgctgc tcggccggcc cggaaccagc accgacgggc gtccagacgc agccgacacc
      241 gtcgatcgca agcgcgagtg tgtcccaacc aacgggcacc agcgcgtcgt acgccggcga
      301 gtccgctgtg cccagcacga gcaccgaggg tccggccccg gagaccatcg ccgcgacccc
      361 actgtcgcgc agcgccgtca cgagctggag cgtcgccggc atggccgggc ggcggtactt
      421 ctggtgcagc cagtcccggg tggccgggtg gaggagctcg ggctgaccgg tgagggcggc
      481 caccaacaac ccggcgcgtc cggcgttggc cgaagcggct cggtgcggca cggtctcggg
      541 caggatgccg cgcgccacct ccgtggacac gccggccccg ggaaccagca ccgttgcccg
      601 cacgcgagcg tcgacctcga cgcgggcggc cgaggatccc tgcagatccg tccaggcgat
      661 cgtcaaccct ccgaagaggc aggccgcaac gttgtccgga tgtccctcga gctcgacggc
      721 gagtgagagc gcatcgtcag caccgagctc gacgtctggt gccaacgcct gggcgagtct
      781 gatgccggcc acgatcgcgg ccgccgacga gcccaggcca cgggcgtgcg gaatgcggtt
      841 ctcagccgcc agcgacaagc cgggctgggc gacgccgagc aggtcgaacg tcgcccgcat
      901 cgcgcggatg acgaggtgct gctcgtcgag cgggaccgac tccgccccct cgccggccac
      961 gtcgaccacc aggccggtct cggtcgcggt ggcggccagg tcgtcgtaca ggccgagaca
     1021 caggccgaac gcgtcgaagc cgggtcccag gttggcactc gtcgcgggca ccctcgccgt
     1081 cgcagaacgg cccacgaccg accgggacga aggcacctca gccgtccacc agcccggccg
     1141 cacgcgcagc ctgagcagcg tccttgtcga ccacgagctc ttccggggtg ccgacgtact
     1201 ccagcgcggt gtcgatgtcc ttcagcccgt gtccggtcac cgtgatcgcc acgaccgtgc
     1261 cggggtcgag cagaccgtcg tcatgtcgct tcaggaggcc cgccaccccg gcggccgacg
     1321 ccggctcgac gaagacaccg tccctcgcgg ccatggtccg ttgcgctgcc aggatctgcg
     1381 cgtcgctgac ggcctcgatg atgccgccgg actcgtcacg ggcggcctcg gccagcaacc
     1441 aggacgccgg gttaccgacc cggatggccg tcgcaacggt ttccggcttc ggtaccacgt
     1501 gaccggtgac gatcggcgcg gcgcccgcgg cctggaaccc ccacatggcc ggtcgtcggc
     1561 tcgctcggcc ggtggcggcg tactcgacat accccttcca gtacgccgcg atgttgcccg
     1621 cgttaccgac tgggagcacg tggatctcgg gggcatccgc caagaagtcg acgatctcga
     1681 aggcagccgt cttctgtccc tcgaggcgta cggggttgac ggagttgacg agcgcgacgg
     1741 ggtagtcctt cgagagggtc cgcgcaaggt ccagacagtc gtcgaagccg ccgcgcacct
     1801 ggatgatctt cgccgcgtgc atgatcgcct gtgccatctt gccgcgggcg atcttgcctt
     1861 gcggtaccag cacgatcggc gtcatgcctg ctcgcgacgc gtacgccgcc atcgacgccg
     1921 acgtgttgcc cgtggatgca cacacgaccg ccttggcccc ctgctcgaga gcgttcgaga
     1981 ccgcaacggt catgcctcgg tccttgaagg aacctgtcgg gttgttggcc tcgaccttga
     2041 gccacaccga gcacgcggtg cgttcggaca accaaccgga ctggaccagc ggtgtgccac
     2101 cctcccgcag agtgaccacg ggggtctcgt cgctcaccgg gagccagcgg cggtactcct
     2161 cgatgacccc acgccactgg tgcgtggccg ccgactgcga cgcgtgcagc gtcattccgg
     2221 cgcgccttcc acgcgcatga ccgagctgac gtcccgcacg acgtccagcg aacggagccc
     2281 atccacggtc gcggacaacg cggcgtcggg cgcgctgtgg gtgacgacga cgagctgggc
     2341 gtcgtcacct cgcccctcct gacggaccgc ctggatcgag acctggtgcc gggcgaacaa
     2401 cgtggcaacc gccgccagca ccccgggctt gtcgtccacg tcgatcgcca cgtggtaacg
     2461 ggtgatcgtc tcccccatcg acgcgatctc acacttcgcg tacgtcgact cgccggctcc
     2521 ggtcacggcg gcgagccggt tcctcgcgac ggtgacgacg tcccccagaa cagcactcgc
     2581 ggtcggtcct ccaccggcac ccggaccgta gaacatcagc tgtcccgcgg cctcggtctc
     2641 cacgtacacc gcgttgtagg cctctcggac gctcgccagc ggatgtgccc gcggaatcat
     2701 cgcgggatgc acgcgcacgg acacggtgtc gggccgacct gactcgatcg caccgtcgcg
     2761 gtcgcagatg gcgagcagct tgaccaccga gcccatgtcg cgggccgacg tcacgtcctg
     2821 tgccgacacg tccccgatgc cttcgcggtg cacgtccgcg gccgtgaccc tggtgtggaa
     2881 cgcaaggctc gccaggatcg cggccttggc agccgcgtcg aagccgtcga cgtcggccgt
     2941 cgggtcggcc tcggcgtacc cgagcgactg cgcctgctcg agcgcatcgt cgaagctcgc
     3001 gccggaggtg tccatctggt cgaggatgta gttcgtcgtc ccgttcacaa tgccgagaac
     3061 gcgacggacc ttgtcaccgg ccagtgactc gcgcaacggc cggatgatcg ggatcgcgcc
     3121 ggcgacggcg gcctcgaagt agacgtcgcg tccaaccttg tccgcggtct cgaacagggt
     3181 ggagccgtcc tcggcgagca gcgccttgtt ggccgtcacg acagaggaac cggcctcgag
     3241 cgcagccagg atgagcgagc gcgccggctc gatcccgccg atcacctcga tcacgacatc
     3301 gaggtcgccg cgcgtgacga gtgcgtgcgc gtcggtggtg aacaggcccc gcgggaggtc
     3361 cgccgcgcgc gcgcggtcgg gccgccgtac cgcgatcccg acgagctcca acggggcacc
     3421 gacccgcgcc accagatcat cggtctgctc cagcaggagc cgcgcgacct cggtaccgac
     3481 gacaccgcag ccgagcatgg cgatgcggac gggctcgttc atggacgcct ttccagtgcg
     3541 ggcgtcgcgc ggctgcgacg gcacggtcga gcaatggcgc ggtgc
//
LOCUS       DATBFZ010000549         9808 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_6209456, whole genome shotgun sequence.
ACCESSION   DATBFZ010000549 DATBFZ010000000
VERSION     DATBFZ010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 9808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9808
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6209456"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="VH419_16715"
     CDS             complement(<1..272)
                     /locus_tag="VH419_16715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008363779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEY7045315.1"
                     /translation="MAIHPQRSSGMPFTRYRPFPPIDLPDRSWPTRTISHAPRWLSTD
                     LRDGNQALIDPMTPGRKQRMFDLLVELGYKEIEVGFPSASQFDFDFV"
     gene            complement(607..882)
                     /locus_tag="VH419_16720"
     CDS             complement(607..882)
                     /locus_tag="VH419_16720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7045316.1"
                     /translation="MTKRSEAELDAKAAEFEQWAETLDPESADFERVDDLRAVAEAAD
                     AVAAAQANLNAQVAQARAKGGSWGRIGIALGVSRQAARQRYSDLVLR"
     gene            1376..2374
                     /locus_tag="VH419_16725"
     CDS             1376..2374
                     /locus_tag="VH419_16725"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY7045317.1"
                     /translation="MDLERIDPRGLDDATASQIADAMNATARAVGQKVAPMTAENLIA
                     QATYTHDDRPYDAMWLAREDSREVVGTAWVELPHWDNQHLGLAFCVVHPDSWGRGIGT
                     ALLDAQAGFAREQGRTMLLTFCLRDKPAHALLTSYGFEVGQSQAQRRLYPRELDYGRI
                     QTLADDAAAKAGGYELVRLDGPAPDDWLDELVNIFEAINDAPNDDIEITPDVFSVERI
                     RQYESAMSHRRSHLYRLMARHRDSGEWAGHTILCVDELRPGMAFQEDTTVVARHRGHR
                     LGMWLKATMLLWMRDLGVDLETIDTWNAESNAHMIAVNDELGCIVNARGLALQLHL"
     gene            2456..3352
                     /locus_tag="VH419_16730"
     CDS             2456..3352
                     /locus_tag="VH419_16730"
                     /inference="COORDINATES: protein motif:HMM:NF025215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation family protein"
                     /protein_id="HEY7045318.1"
                     /translation="MPRPLPLRLGLALVALVVAALLAGCGVLDTSSGASTATPDDGAA
                     SSPGAPIRSRSADLSPPREPAAAPEVGECRVLSYADIGRFSNSSSTVRCDRPHTGYTF
                     AVAQLPKDIAFEGVDIGNDAVQDAAAGLCRTAFASYVGGSAATHRLSRLSSTYFLPGQ
                     TDFDLGARWVRCDAIALQSERVLADLPGRLEGFNDSDAALADYGLCARGEPGTRDGVL
                     VMCNQKHTFRALTALRLGAEDAAYPGDNVVADGGQQRCDDFISRTLGLGGGYSYTWTY
                     PSRDDWANGQRFGFCWMKSSAA"
     gene            3397..4329
                     /locus_tag="VH419_16735"
     CDS             3397..4329
                     /locus_tag="VH419_16735"
                     /inference="COORDINATES: protein motif:HMM:NF025215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation family protein"
                     /protein_id="HEY7045319.1"
                     /translation="MGRAFGGPQVIGSAVVALILTACGGAQDVANGPDDTTETTRSAA
                     PSSTSTTRSAEPSTPTSDPASAPTTTPTTTPTSQPLAGRPQVGDCYDTSRRAFARQND
                     GSFPVSCARPHTAETFAVVTVGPLPSARAIDKVWRHCQPRFKTYVGGSPTISTIGFTV
                     MLPSTAQAAAGQGWIRCDAMEQPSYNGHRGITRRGSLQDALSSGVPAKYRGCALHWPK
                     VDHPVIFSSCKRRHQAELIPESLHLGGPEAKYPGLKASRSRSRQFCESTFQDYVTETL
                     NYYFYFPTPASWRSGSHDTACWALDIHGDGLPPI"
     gene            complement(4427..5362)
                     /gene="era"
                     /locus_tag="VH419_16740"
     CDS             complement(4427..5362)
                     /gene="era"
                     /locus_tag="VH419_16740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014156366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEY7045320.1"
                     /translation="MTESASTPIFRSGFASLVGRPNTGKSTLTNALVGSKVAITSSRP
                     QTTRHAVRGIVHRPDAQLVLVDTPGLHKPRTLLGERLNDLVKATWAEVDVCAVCLPAS
                     DRIGPGDRFLVSELAKVKRTPKIAVATKADLVTPDRMAEHLAGIAELGAVANVEWAEV
                     IPVSALRGDQVDLLSDLLVSRLPEGMPLYPEGELTDEPEETLVAELIREAALEGVRDE
                     LPHSIAVVVEEMGLRPGRPDDQPLLDVHANLFVERSSQKAIVIGRQGGRLKNVGTTAR
                     RHIEALLGTPVYLDLRVKIAKDWQRDPKQLRRLGF"
     gene            complement(5466..5813)
                     /locus_tag="VH419_16745"
     CDS             complement(5466..5813)
                     /locus_tag="VH419_16745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020386033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEY7045321.1"
                     /translation="MPIELTDPEDAKLLTLARTSLARAGASESAAVRDVDGRTYVSTN
                     VDLPSLQLTALQVAVSMAISSGVTGLETALVVNDLLTLAESDLAVVRDFAGAGVPVFR
                     ADASGQVIDQVTT"
     gene            complement(6013..7377)
                     /locus_tag="VH419_16750"
     CDS             complement(6013..7377)
                     /locus_tag="VH419_16750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020386034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEY7045322.1"
                     /translation="MTSSDVLLLVIAAILVVFGGVLASADAALSTISKVRAEELARHR
                     RRGAGRLLVILADAPRFLNTALLLRILCETTATVLVTLVLVDLLDPTWLAVVTAAGSM
                     IVVSYIAVGVGPRTLGRQHAERVALVTARPIAALTAVLGPLTQLLIMIGNAITPGRGF
                     PEGPFATEAELRELVDLAEQSRVIESDESRMIHSVFELGDTLAREVMVPRTDIVFIER
                     YKTLRQMISLALRSGFSRIPVVGDGLDDVVGIAYLKDVTKRVFDRHEAESTERIETVM
                     RPCIFVPDSKPADELMREMQAKRTHVAIVVDEYGGTAGLVTIEDLLEEIVGEITDEYD
                     VEPVEVERLADGAVRVSSRMPVDELAEIFDVEIEDEDVETVGGLMAKHLGKVPIPGAE
                     VVCEGLHFQAESLAGRRNKVVTVVVTPADRRTAKEAAKDEPAAAADANGIAPDADSHP
                     VANP"
     gene            complement(7419..7889)
                     /gene="ybeY"
                     /locus_tag="VH419_16755"
     CDS             complement(7419..7889)
                     /gene="ybeY"
                     /locus_tag="VH419_16755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEY7045323.1"
                     /translation="MSVEVLNESGIDLDVAEFAKLARFTMSRMRVHPEAELCLRLVDE
                     ATITEYNQHWMGKEGPTDVLSFPMDELRPAREGEVEEEGILGDMLLCPQYAAAQAPSF
                     SRSVDDEMHLLTIHGILHLLGYDHAEPEEEREMFALQARLLAEWLGVKHGDRVE"
     gene            complement(7900..8946)
                     /locus_tag="VH419_16760"
     CDS             complement(7900..8946)
                     /locus_tag="VH419_16760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007078270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEY7045324.1"
                     /translation="MTQQPVDQAGPRHVIVIPNSVPVVAVLGPQDEFLSLIEREFKAE
                     VAVRGNEISLVGDRGEVALVERLIDELVTMVRTGQGLTSETVERVIGMLRAETEERPA
                     DVLTLNILSNRGRTIRPKTLNQKRYVDAIDKHTVVFGIGPAGTGKTYLAMAKAVQALQ
                     AKHVNRIILTRPAVEAGERLGYLPGTLSEKIDPYLRPLYDALHDMLDPEKIPKLLTAG
                     TIEVAPLAYMRGRTLNDAFIILDEAQNTSPEQMKMFLTRLGFGSKMVVTGDITQVDLP
                     SGTRSGLRVVREILTGIKDISFCMLTAQDVVRHRLVGKIVGAYDDYEARGGYGGHAAH
                     AHRPHGPTSVVEGG"
     gene            complement(9036..9386)
                     /locus_tag="VH419_16765"
     CDS             complement(9036..9386)
                     /locus_tag="VH419_16765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HEY7045325.1"
                     /translation="MTAQQDCLFCRIVSGEVPSDTVAENERTVAFRDIDPKAPTHILV
                     IPRVHVPTLPELAESHPDDALAVLAMARQVAESDGVGDGYRLTANNGAPAQQSVFHAH
                     VHVLGGRTFSWPPG"
     gene            9593..>9808
                     /locus_tag="VH419_16770"
     CDS             9593..>9808
                     /locus_tag="VH419_16770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020393326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7045326.1"
                     /translation="MTSLSGVPALALDAGRPAAVTDGGATMAADRWDADEAVTQLYAA
                     HYTALVRLAALLVRNSGEAEEIVQDSFV"
BASE COUNT         1543 a         3362 c         3272 g         1631 t
ORIGIN      
        1 acgaagtcga agtcgaactg gctggctgag gggaagccga cctcgatctc cttgtagccc
       61 aactcgacga gcaggtcgaa catgcgctgt ttgcgccccg gcgtcatcgg gtcgatcagc
      121 gcctggttgc cgtcccgcag gtcggtcgac agccagcgtg gcgcgtggct gatcgtgcgg
      181 gtgggccagc tgcggtcagg aaggtcgatg ggtgggaagg ggcggtagcg ggtgaacggc
      241 ataccgctgg atcgctgagg gtggatcgcc atggcggggc tgctttcgaa acggaatggg
      301 agccggcggg cagcagcaca tctccgcggc gagggtccgg ctaccgaacc tcgccgcggc
      361 acccaagcag caggctcgag cctgcgtggt ccgtcatggc ccgaccaggc tagcaccggt
      421 acgccgtccg acgcttcccg ccttcccgcc cgctgagacc cgacggcgga cgagtcgtcg
      481 aagtcaccgc cctcaccaga ctcgacggcg tgtacgtcgc gatcgaacgg gtccgcaggc
      541 aggaacgaag aggctcgatc acatccggtc gggatacatc acctcactcg cggcggtgat
      601 gagcggctac cgcaggacca gatcgctgta ccgctgccgc gcggcctggc gagacacacc
      661 cagcgcgatc ccgatccgtc cccagctgcc gcctttggcc cgcgcctgcg ccacctgcgc
      721 gttgaggttg gcctgcgcag cggccaccgc gtcggccgcc tccgccaccg cccgcaggtc
      781 gtcgacgcgc tcgaagtccg cagactccgg gtccagggtc tcggcccact gttcgaactc
      841 ggccgccttc gcgtcgagct cagcttcgct tcgcttcgtc atcgtgtcat cacctccttc
      901 gcaggaatcg ttggaacgcc ggacgcagga ccatcgcgtg gatgatcacc gggtcgtcgt
      961 cgggatcgac cacgcccacc tcgagcagtc gcccggtccg gtccgatgag catcagcagg
     1021 gtcgtcggcc cgggcacgtc cgtcatcgcg tgccgtgccg catgcagcat gtcctcgtcg
     1081 cggatcccgt gttgccgggc ggtgtggtgg atcctcacat cagtgacaag cgtagttgtc
     1141 agcgtgcgtc atgacaagga gtcttgtcat gacctggcgt cacagcccgg tcaccgtcat
     1201 ggggctcgag catgtagtgt ccgtcaagcg tgtacgcccc cgcaccgcag ggctcactac
     1261 gctccctcgg ccgcagccgc ctcctggcac tagcggtgtt ctcagcacag ttcgggctga
     1321 acacgtcgcc gtcacgtcgt gttgccgagt tgggtcggag gccaaaggat ggagcatgga
     1381 cctcgaacgc atcgaccccc gggggttgga cgatgcgacc gcgtcgcaga tcgccgacgc
     1441 gatgaacgcc actgcgcgtg ccgtcggcca gaaagtggcg ccgatgacgg ccgagaacct
     1501 gattgcgcag gcgacgtaca cccacgacga ccggccgtac gacgccatgt ggctggcccg
     1561 tgaggacagt cgtgaggtgg tcgggacggc atgggtggag ctgccacatt gggacaacca
     1621 gcatctcggc ctcgccttct gtgtcgtgca tcccgattcc tggggccgcg gcatcggcac
     1681 cgccttgctc gacgcgcagg cgggattcgc ccgggagcag ggtcgcacga tgctgctgac
     1741 gttctgcttg cgggacaagc ctgcgcacgc gctgctcacg tcctacggct tcgaggttgg
     1801 ccagtcgcag gcgcagcggc ggctctaccc acgcgagctg gactacggcc gcatccagac
     1861 actggccgac gacgcggccg cgaaagccgg cggttacgag ctcgttcgcc tcgacggacc
     1921 ggcaccagac gactggctgg acgagctcgt caacatcttc gaggcgatca acgatgcacc
     1981 gaacgacgac atcgagatca cacctgacgt gttcagcgtc gagcgcatcc gccagtacga
     2041 gtccgcgatg tcgcaccgac gttcgcacct gtaccggttg atggcgcggc accgggacag
     2101 tggcgagtgg gccgggcaca cgatcctctg cgtggacgag ctgcgaccag gcatggcgtt
     2161 ccaggaggac accacggtgg tcgcccggca tcgtggccac cggctcggga tgtggctcaa
     2221 ggccaccatg ttgctgtgga tgcgcgacct cggggtcgac ctcgagacca tcgacacctg
     2281 gaatgccgag tcgaacgcgc acatgatcgc ggtcaacgac gagctcgggt gcatcgtcaa
     2341 cgcacgcggt ctcgcactcc agctgcacct gtgaaccgcc cgtccggaag ccgtcacgcg
     2401 cgccgctgct gacctccaca gatcggcgct tcgccgtggt tacagtggtc ggaccatgcc
     2461 tcgccccctg ccgcttcgcc tcgggctcgc cctggtcgcc ctcgtcgtcg ccgctcttct
     2521 cgcaggctgt ggcgtgctgg acacctcgtc cggcgccagt acggcgacac ccgacgatgg
     2581 cgctgcgtca tcacctggtg cgccgatccg gtcgaggtcg gccgacctgt caccgccgcg
     2641 tgagccggcc gctgcgcccg aggtcggaga gtgtcgtgtg ctgagctacg ccgacatcgg
     2701 ccggttctcg aactccagca gcacggtccg gtgcgaccgg ccgcacaccg gttacacgtt
     2761 cgccgtggcg cagctcccga aggacatcgc gttcgagggc gtcgacatcg gcaacgatgc
     2821 cgtccaggac gcggcggccg ggctgtgccg gacagcgttc gcgtcgtacg tcggcgggag
     2881 tgccgcgacc catcggctct ctcggctgtc gtcgacctac ttcctgcccg gccagacgga
     2941 cttcgacctc ggcgcccgct gggtgcgctg cgacgcgatc gcccttcagt ccgagcgtgt
     3001 actggcggat ctgcccggca ggctcgaggg cttcaacgac tccgacgccg ccctcgccga
     3061 ctatggtctc tgcgcgcggg gcgaacccgg cacgcgcgac ggcgtcctcg tgatgtgcaa
     3121 ccagaagcac acgttccgag ctctgacggc gctgcgcctc ggtgcggagg acgcggcgta
     3181 ccccggtgac aacgtcgtcg ccgacggcgg ccagcaacgc tgcgacgact tcatctcccg
     3241 cacgctcggc ctcggcggcg gctactcgta cacgtggacg tacccgtcac gtgacgactg
     3301 ggccaacggt cagcggttcg gtttctgttg gatgaagtca tccgccgcct gaccgactga
     3361 tcatgtgctg ggcagacagg ctggcatgat cgacgcatgg gtagggcctt cggggggccg
     3421 caggtcatcg gctcggcggt ggtggcgctg atcctcaccg cctgtggcgg tgcacaggac
     3481 gtcgcgaacg gtcccgacga caccaccgag acaacccgca gcgcggcgcc gagcagcact
     3541 tcgacgacga ggtcggcgga gccgagcacg ccgaccagcg accccgcgtc ggcaccgacc
     3601 acaacaccga ccacaacacc gacctcccag ccgcttgccg gtcgaccaca ggtcggcgac
     3661 tgctacgaca cgagcagacg tgcgttcgcc cgacagaacg acggcagctt cccggtgtcc
     3721 tgcgcacgac ctcacacggc tgagacgttc gccgtggtca ccgtcggtcc gctgccgtcg
     3781 gcacgcgcga tcgacaaggt gtggcgacac tgccaaccac gcttcaagac gtatgtcggc
     3841 gggtccccga cgatcagcac catcgggttc acggtgatgc tgccgagcac agcgcaggcc
     3901 gccgccggcc agggctggat ccgttgcgat gcgatggaac agccgagcta caacggacac
     3961 cgaggcatca ctcgacgcgg cagcctccag gacgcgctct ccagcggcgt gccggccaag
     4021 taccgaggct gcgcactgca ctggccgaag gtcgatcacc cggtgatctt ctcctcgtgc
     4081 aagcgacgcc accaggccga gctcatcccc gagtcgctcc atctcggcgg gccggaggcg
     4141 aagtaccccg ggctgaaggc gtcacgcagc cggagcaggc agttctgtga gtcgacgttc
     4201 caggactacg tgaccgagac gctcaactac tacttctact tcccgacccc ggcgtcctgg
     4261 aggtccggct cgcacgacac cgcctgctgg gcgctcgaca tccacggtga cggcctgccg
     4321 ccgatctgac gcatcaccgc gctccgctgg ttgagcctgt cgaaaccaag ggagtcaggt
     4381 ggcttcttga gccggacccg gcgactccgt cgctcggctc cgtccatcag aagccgaggc
     4441 gccgcagttg cttcgggtcc cgctgccagt ccttggcgat cttgacccgc aggtcgaggt
     4501 agaccggcgt accgagaagt gcctcgatgt gacgacgggc cgtcgtgccc acgttcttca
     4561 gcctgccgcc ctgccgtccg atcacgatcg ccttctggct ggaccgttcg acgaacaggt
     4621 tggcgtggac gtcgagcagc ggctggtcgt ccggccgtcc cggtcgaagt cccatctcct
     4681 cgaccaccac cgctatggag tggggaagct cgtcccggac accttcgagg gctgcttccc
     4741 tgatcagctc cgcgaccaat gtctcttcgg gttcgtcggt cagctcgccc tcggggtaca
     4801 gcggcatacc ctccgggagc cggctgacca gcaggtcact cagcaggtcg acctggtcgc
     4861 cgcggagcgc cgacaccggg atcacctcag cccattcgac gttcgcgact gcgccgagtt
     4921 cggcgatacc ggccaggtgc tcagccatcc ggtcgggcgt gaccaggtcg gcctttgtcg
     4981 cgacggcgat cttcggcgtg cgcttcacct tcgccagttc cgagaccagg aaccggtcac
     5041 ccggtccgat ccggtcgctc gccggcaggc acaccgcgca cacgtcgacc tccgcccagg
     5101 tcgccttcac caggtcgttg aggcgctcgc cgagcaacgt ccgcggcttg tgcagacctg
     5161 gtgtgtccac gagcaccagc tgcgcgtccg gccggtgcac gatgccgcgc actgcgtggc
     5221 gcgtcgtctg cggccgcgat gacgtgatcg cgaccttcga accgacgagt gcattcgtca
     5281 gcgtggactt gccggtgttg gggcggccca ccaggctggc gaagccactc cggaagatcg
     5341 gcgtcgacgc agactcggtc acctgaccga ttgtgtcgtg ccccccggga ttgcgggaaa
     5401 cgtgcggtga aaaggtacgc cgagggaaga cctttcccgc aattcgagga agccctcgcc
     5461 gacggtcagg tggtgacctg gtcgatgacc tggccggacg cgtcggcgcg gaagacgggt
     5521 acgccggcac cggcgaagtc gcggacgacg gcgaggtctg actcggcgag cgtgaggaga
     5581 tcgttgacca ccaacgccgt ctccagcccg gtcactccac tggagatggc catggacacc
     5641 gccacctgca acgcggtcag ctgcaacgac gggaggtcga cgttcgtcga gacgtacgtg
     5701 cggccgtcga cgtcacgaac cgcggcggac tccgacgccc cggcccgcgc caagctcgtc
     5761 cgcgccagcg tgagcagctt cgcgtcctcg ggatccgtca gctcgatcgg catccagcga
     5821 gcctagtgcc gcgtcaacca acgttgctcg tcgtgagcgg tgtccgggtg gatgagctgc
     5881 aaggcagagg agggaggcga tgcgcagcat cgttgaccga cgataacgcc gcagatcgcc
     5941 gcccgggcga ccgcgcaaca gggcgaatgt tggtcgacgg ggtacaaggc aggggttgac
     6001 tgtccgtgcg actcaggggt ttgccacggg gtgcgagtcg gcgtccgggg caatgccgtt
     6061 cgcgtcggcg gccgcggccg gctcgtcctt cgccgcctcc ttggcggtcc gtcggtcggc
     6121 cggcgtgacg acgaccgtca cgaccttgtt ccggcgaccc gccagcgact ccgcctggaa
     6181 gtgcagaccc tcgcagacga cctcggcgcc cgggatgggg acctttccga ggtgcttggc
     6241 catcaagccg cccaccgtct cgacgtcctc gtcttcgatc tccacgtcga agatctccgc
     6301 cagctcgtca acgggcatcc gcgagctgac ccggacggcc ccgtcggcaa ggcgttcgac
     6361 ctcgaccggc tcgacgtcgt actcgtcggt gatctcgccg acgatctcct cgagcaggtc
     6421 ctcgatcgtc accagcccgg cggtgccgcc gtactcgtcc acgacgatcg caacgtgcgt
     6481 gcgcttcgcc tgcatctccc gcatgagctc gtcggcgggc ttggagtcgg gtacgaagat
     6541 gcacggccgc atcacggtct cgatccgctc ggtcgactcc gcctcgtgcc ggtcgaagac
     6601 ccgcttggtg acatccttga ggtacgcgat gccgacgacg tcgtcgagac cgtccccgac
     6661 gaccgggatc cgtgagaagc cgcttcgcaa cgccagcgag atcatctgtc gaagcgtctt
     6721 gtagcgctcg atgaacacga tgtcggtccg cggcaccatc acctcgcggg ccagtgtgtc
     6781 gccgagctcg aagaccgagt ggatcatcct gctctcgtcg gactcgatga cgcgcgactg
     6841 ctcggcgagg tcgacgagct cgcgcagctc ggcctcggtg gcgaacggac cctccgggaa
     6901 gccgcggccg ggcgtgatgg cgttcccgat catgatcagc agctgggtga gtggcccgag
     6961 cacagcggtc agggcggcga tcggccgagc cgtcacgagt gcgacccgct ccgcgtgctg
     7021 acggccgagc gtacgcgggc cgacgccgac cgcgatgtag gacacgacga tcatcgagcc
     7081 cgccgccgtc accaccgcga gccacgtcgg gtcgagcagg tcgacgagga cgagcgtgac
     7141 gaggacggtc gcagtcgtct cgcagaggat ccgcagcagc aacgcggtgt tcaggaaccg
     7201 gggggcgtca gcgaggatga cgagcaagcg cccggcaccg cgtcgacggt gacgggccaa
     7261 ctcctcggcg cgcaccttcg agatcgtcga gagcgccgcg tcggcgctcg ccagcacacc
     7321 gccgaacacg accaggatcg ccgcgatcac gagcagcagc acgtccgacg acgtcatcag
     7381 gcgttcaccc tcaccgggaa atcactgtca tcggcggctc actcgacgcg gtcgccgtgc
     7441 ttcacaccga gccactccgc gagcagccga gcctgcagcg cgaacatctc acgctcctct
     7501 tccggctccg cgtggtcgta gccgagcagg tgcaggatcc cgtggatcgt cagcaggtgc
     7561 atctcgtcgt cgacgctacg cgagaaggac ggtgcctgcg cagcggcgta ctgcggacag
     7621 agcagcatgt cgccgaggat gccctcctcc tccacttctc cctcgcgcgc cggacgcagc
     7681 tcgtccatcg ggaacgacag cacgtcggtg gggccctcct tgcccatcca gtgctggttg
     7741 tactccgtga tcgtcgcctc gtcgacgagt cgcaggcaca gctcggcctc tgggtgaacg
     7801 cgcatccggc tcatcgtgaa gcgggccagc ttggcgaact ctgcgacgtc gaggtcgatc
     7861 cccgactcgt tgagcacctc gacgctcacc gccggaccgt cacccaccct cgacgaccga
     7921 cgtcgggcca tgcggtcggt gcgcgtgcgc cgcgtgtccg ccgtagccgc cgcgggcttc
     7981 gtagtcgtca tacgcgccga cgatcttccc gacgagccga tggcggacga cgtcttgtgc
     8041 cgtgagcatg cagaaactga tgtccttgat gccggtcagg atctctcgga cgacgcgcag
     8101 cccgctgcgt gtcccgctcg gcaggtcgac ctgggtgatg tcaccggtga cgaccatctt
     8161 cgagccgaag ccgagccggg tcaggaacat cttcatctgc tcaggagagg tgttctgcgc
     8221 ctcgtcgagg atgatgaacg catcgttgag cgtgcgacca cgcatgtacg cgagcggcgc
     8281 gacctcgatc gtcccggccg tgagcagctt ggggatcttc tcggggtcga gcatgtcgtg
     8341 cagcgcgtcg tacagcggcc gcaggtacgg atcgatcttc tcgctcagcg tgccgggcag
     8401 gtagccgagc cgctcgcctg cctcgacggc aggtctggtg aggatgatcc ggttgacgtg
     8461 cttggcctga agcgcttgga cggccttggc catcgcgagg taggtcttgc cggtgcccgc
     8521 cggaccgatg ccgaagacga cggtgtgctt gtcgatggcg tcgacgtacc gcttctggtt
     8581 cagcgtcttg ggtcggatcg tgcgaccgcg gttgctcagg atgttgagcg tgagcacgtc
     8641 cgcaggacgc tcctccgtct ccgcccgcag catgccgatg acccgctcga cggtctccga
     8701 cgtcaaccct tgaccggtcc gaaccatcgt gacgagctcg tcgatgagcc gctcgacgag
     8761 cgcgacctcc ccgcgatctc cgacaaggct gatctcgttg ccgcggacgg cgacttcggc
     8821 cttgaactcg cgctcgatga gggagaggaa ctcgtcttgg gggccgagta cggcgacgac
     8881 cgggacgctg ttggggatca cgatcacgtg gcgtggtccg gcctggtcta cgggttgctg
     8941 tgtcatcgtg atgtccatgc tacgggcatc acccgacccg tttcacgctg atttggtccg
     9001 atgcgcaggt cagcgcgtac gtcttctcgg agaggtcacc ccggcggcca gctgaacgtg
     9061 cggccgccca ggacgtgcac gtgagcatgg aagaccgact gctgggccgg cgcaccgttg
     9121 ttcgcggtca gccggtagcc gtctccgaca ccgtctgact ccgcgacctg acgggccatg
     9181 gccaagacgg cgagcgcgtc gtccgggtgg gactcggcga gctccggcag cgtcgggacg
     9241 tgcaccctcg ggatcaccag gatgtgcgtc ggcgccttcg ggtcgatgtc ccggaacgcc
     9301 acggtccgct cgttctccgc caccgtgtcg ctcggcacct cgccgctcac gatccggcag
     9361 aacaggcagt cctgctgcgc cgtcatcacc tctccctccg ttcggtcgtt gagtgctggg
     9421 ttcttggttg ttgagtgctg ggttttcggt tgttgagtgc tgggttctcg gtcaatgagt
     9481 gctgggttgt gggtcaatga gtgaccgtgt tggtgcaaga cggccgagca gcgtacgccg
     9541 atcatggtga cgagcgtgtc aaccgaaccg cggggttgcg cgtgtgttgg acatgacgag
     9601 cctatccggc gtaccggcac ttgcgctgga cgccggtcgc cccgcggccg tgaccgatgg
     9661 aggcgccacg atggcggctg accgctggga cgccgacgag gcggtgaccc agctctacgc
     9721 ggctcactac accgcgctcg tccggctggc cgccttgctg gttcgcaaca gcggcgaggc
     9781 ggaggagatc gtgcaggact cgttcgtc
//
LOCUS       DATBFZ010000550         2685 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_5227017, whole genome shotgun sequence.
ACCESSION   DATBFZ010000550 DATBFZ010000000
VERSION     DATBFZ010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2685
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5227017"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..822)
                     /locus_tag="VH419_16775"
     CDS             complement(<1..822)
                     /locus_tag="VH419_16775"
                     /inference="COORDINATES: protein motif:HMM:NF018436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1206 domain-containing protein"
                     /protein_id="HEY7045327.1"
                     /translation="MSRTIGNPARGAGAEAALAAGRLNDSVWFERGIRLGLVAYGVIH
                     ILIGWLALQLAFGDRSGSPDQQGALHQVAQQSLGDPLLWLIGVGLLVLAIWQVFEAIW
                     GHRKRDEPKRTFKRISSAGRVVLYAVLGVTAIRMAVQTSSSGGSSTDSMTAKLMDQTA
                     GRWLVAAVGILIWLVAVSQVKRGVTKSFTHDLTGDAVSGDSGTAVEKLGKVGYVAKGV
                     ALTVVGALFVWAAWDHDPKKAGGLDVALRKILDQTFGPVLLTVVAAGIICFGLYCF"
     gene            complement(884..>2685)
                     /locus_tag="VH419_16780"
     CDS             complement(884..>2685)
                     /locus_tag="VH419_16780"
                     /inference="COORDINATES: protein motif:HMM:NF023326.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3418 domain-containing protein"
                     /protein_id="HEY7045328.1"
                     /translation="PHWSKKRGAVMAYEKVTLYGVPVVTDRLVNYARIDPETARELFI
                     RHALVGGEWQTHHRFFAANQQLLEEAEELEHRARRRDIVVDDETIFDLYDARVPAGIV
                     STAHFDAWWKKERHAHPDLLTFTMDMLVRDAAEQVSDEDFPEVWRHGDAELTLDYQFE
                     PGSEADGVTVDIPVATLNQVEADDFSWPVPGLREELVVALLRSLPKTLRRQVVPAPNH
                     ARAFLAATVPGEEPLLDALERYLRRTVGVVVSRDAWDVDKIPPHLRLSFRIVDSTGTV
                     VGEGKDLARLRDDLRDESGAAISAAAATVERAGLTTWDFDELPRTVVVTRAGHEVRGY
                     PALVEERHSDGSEPNAVAIRVLATEGEQVTTTRIGVRRLLVNAVPAAVRDIVGGLTNQ
                     QRLTLGLAPHPTIPAVLEDAYAAAVDEVMLETYAGLAWNRAEFDRLVDAVRREGPRRT
                     DEVVQVLLRILPAAHDVHRRLSGRADLSLLPALSDMKGQWERLLHPGFVAAAGLARLR
                     HYPRYLSAMDVRLDKLHRDPGRDAVLMATVAGVQSAYLDRIGALPGDQPPSEALRDIA
                     WMIEELRVSTWAQELKTAQPISVQRVEKALRDL"
BASE COUNT          427 a          963 c          883 g          412 t
ORIGIN      
        1 gaagcagtac aagccgaagc agatgatgcc ggctgcgacc accgtcagga gcaccggccc
       61 gaacgtctgg tcgaggatct tgcgcagggc gacgtcgagt ccacccgcct tcttcgggtc
      121 gtggtcccac gccgcccaca cgaacagcgc cccgacgacc gtgagcgcca cccctttggc
      181 gacgtatccg accttgccca gcttctccac ggcggtaccg gagtcccccg agaccgcgtc
      241 accggtcagg tcatgggtga acgacttggt gacgccacgc ttgacctgac tcacggcgac
      301 gagccagatg aggatcccca ccgccgcgac cagccatcgc ccggctgtct ggtccatgag
      361 cttcgcggtc atcgagtcgg tgctgctccc tccgctcgag ctcgtctgca ccgccatccg
      421 gatcgcggtc acgccaagca cggcgtacag cacgacccga ccggccgagc tgatccgctt
      481 gaacgttcgt ttcggctcat cccgcttgcg gtgaccccag atcgcctcga agacctgcca
      541 gatcgcaaga accaggagtc cgaccccgat cagccacagc agcgggtcac cgagcgactg
      601 ctgggcaacc tgatgcaacg cgccctgctg gtccggcgaa cccgaccggt caccgaacgc
      661 cagctgcagt gccagccagc cgatcaggat gtggatgacg ccgtaggcga ccagaccgag
      721 ccggatccca cgctcgaacc acacactgtc gttcagccgt ccggccgcca gtgctgcctc
      781 tgcacccgcg ccacgagccg ggttgccgat cgttctcgac atcggacctc ccacgtgccg
      841 ggttcccgtt cccgggcgag tccaaacggg aaccccgaag gcgtcagagg tcgcggaggg
      901 ctttttcgac gcgctgcacg gagatcggtt gcgcggtctt cagctcttgc gcccatgtgc
      961 tcacccgaag ctcctcgatc atccatgcga tgtcgcgcag ggcctcgctg ggcggttggt
     1021 ccccaggcag cgccccgatc cggtcgaggt acgccgactg aacgccggcg acggtggcca
     1081 tgagtacggc gtcccgtcca gggtcacggt gcagcttgtc gagacggaca tccatcgcag
     1141 acagatagcg cgggtaatgc cgcagccggg cgagaccggc cgcggcgacg aaacccggat
     1201 ggagaagccg ctcccactgg cccttcatgt ccgacagggc gggcagcaag gacaggtccg
     1261 ctcgaccgga caaccggcga tgaacgtcgt gcgccgccgg caggatccgc agcagcacct
     1321 gcacgacctc atcggtccga cgtggacctt cgcggcgtac cgcatccacc aatcggtcga
     1381 actcggctcg gttccaagcc aggccggcat acgtctccag catcacctcg tcgacggcgg
     1441 cggcgtacgc gtcctcgagg acggccggga tcgtcggatg cggcgcgagc ccgagcgtca
     1501 gcctctgctg gttggtgagt ccgccgacga tgtcgcgcac ggcggccggc acggcgttga
     1561 ccaggagtct gcgtacgccg atccgggtgg tcgtgacctg ctcaccttcg gtggcgagaa
     1621 cgcggatcgc caccgcgttc ggctccgagc cgtcgctgtg tcgctcctcg acgagcgcgg
     1681 ggtagccgcg gacctcgtga cctgcccgcg tcacgacaac ggtgcgcggc agctcgtcga
     1741 agtcccaggt cgtgagtccc gcccgttcga ccgtggcggc ggccgccgag atggcggctc
     1801 cggactcgtc gcgcaggtcg tcgcgcaggc gggccaggtc cttgccctcg cccaccacgg
     1861 tcccggtgga gtcgacgatc cggaaggaca gccgcaggtg cggcgggatc ttgtcgacgt
     1921 cccaggcatc gcgcgagacg accactccga cggtacgccg gaggtaccgc tcgagcgcgt
     1981 ccagcagcgg ctcctcacca gggacggtcg cggccaggaa cgcgcgtgca tggttcggcg
     2041 caggcacgac ctgccggcgc aacgtcttcg gcagcgacct cagcaacgcc acgaccagct
     2101 cctcgcgcag ccccggcacc ggccaggaga agtcgtccgc ctccacctgg ttgagtgtcg
     2161 ccaccgggat gtcgacggtg acgccgtcgg cctccgagcc cggctcgaac tggtagtcga
     2221 gcgtcagctc ggcgtcgccg tgccgccata cctcggggaa gtcctcgtcg ctcacctgct
     2281 cggcggcgtc gcggacgagc atgtccatcg tgaacgtcaa caggtccggg tgggcgtgcc
     2341 gctccttctt ccaccaggcg tcgaagtgcg ccgtcgagac gatgccggcc gggacgcggg
     2401 cgtcgtacag gtcgaagatc gtctcgtcgt cgacgacgat gtcgcggcgc cgcgcccggt
     2461 gctcgagctc ctcggcctcc tccaggagtt gctggttggc ggcgaagaac cggtggtggg
     2521 tctgccactc cccgccgacg agcgcgtgcc ggatgaacag ttcgcgggcg gtctcggggt
     2581 cgattcgcgc gtagttgacc agccggtcgg tcaccaccgg tacgccgtac aacgtcacct
     2641 tctcgtaggc catgacggcg ccacgcttct tggaccagtg cggct
//
LOCUS       DATBFZ010000551         2687 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_5554424, whole genome shotgun sequence.
ACCESSION   DATBFZ010000551 DATBFZ010000000
VERSION     DATBFZ010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2687
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5554424"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(53..610)
                     /locus_tag="VH419_16785"
     CDS             complement(53..610)
                     /locus_tag="VH419_16785"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY7045329.1"
                     /translation="MVRRADQMDATRERIIEATVHLHGTVGPAHTTVAGIAERAGVTR
                     LTVYRHFPDEDTLFEACTSHWRAQQRMPDVDAWMQIPGPVERVAAALEDVYRFFSEAE
                     TMLTLTARDREALPSFVHERNRQVLNAQTAAVLDAWPQRQRTKLRRRLIGHALTFSTW
                     RSLSREQGLTRRDAVAAMTRLVVEC"
     gene            729..1244
                     /locus_tag="VH419_16790"
     CDS             729..1244
                     /locus_tag="VH419_16790"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY7045330.1"
                     /translation="MAQLDAPPLTVRKPGEGRAGGLVPGLGVIFKLDTADTNGMVSVV
                     EHPFDVGVLVPPHIHHREDEYSIVTEGQIGFRSGDREVVLGAGGYITKPRGEVHAMWN
                     AGAVPARMIEIISPGGFEGFFTELSELVASGPPPAEARLEIADRYGLEFAEPSWLPEI
                     IERYGLTPPPG"
     gene            complement(1368..1613)
                     /locus_tag="VH419_16795"
     CDS             complement(1368..1613)
                     /locus_tag="VH419_16795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7045331.1"
                     /translation="MDERSASEPEPYIEMTSHVRARHGIPDDAARMGLDRNTEEGALV
                     AMAGSLNPAKLSHRIVAWLVLVSFIGPMLFGAVHRIF"
     gene            1789..>2687
                     /locus_tag="VH419_16800"
     CDS             1789..>2687
                     /locus_tag="VH419_16800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HEY7045332.1"
                     /translation="MRAVRVHAYGKDPSLDDVPEPRIEGPLDVIVRIDAAGVCRTDLH
                     IIEGQWAEKSGVTLPYTIGHENAGTVAEVGEAVSNVSVDDKVILHPLVTCGLCRACRA
                     GDDVHCTNSRFPGINDDGGMADLLRTGARSVIRLDAGLEPKDVAALADAGLTAYHAVR
                     KAVPLLYPGTHVVMIGAGGLGHIGLQSLLALTPAEVTVIDRSPDALRKATELGAHHTV
                     TAGDDPDAQVSEVMDVTGGLGAHVVLDFVGEHGTESIGVAMTRDAGSYFVIGYGGHVD
                     IATIDIISREINVIGNLVGSYNDL"
BASE COUNT          456 a          924 c          869 g          438 t
ORIGIN      
        1 accgaccacg ggcggccaga ctccacaccc ggtgacagag ctgaggaagc gctcagcact
       61 cgacgacaag cctggtcatc gccgcgacgg cgtcgcgccg cgtgagcccc tgctccctgg
      121 agagcgaccg ccatgtgctg aacgtcaagg cgtggccgat cagcctccgt ctcagcttcg
      181 tgcgttgccg ctgtggccag gcgtccagca ccgctgcagt ctgcgcgttc agcacctgcc
      241 ggttgcgttc gtggacgaag ctcggaagtg cctcccggtc acgtgccgtc agcgtcagca
      301 tggtctccgc ctcgctgaag aagcggtaga cgtcctccag cgctgctgcg actcgctcga
      361 ccggaccggg aatctgcatc caggcgtcca cgtcgggcat ccgctgctgg gcgcgccagt
      421 gcgacgtaca cgcctcgaac agcgtgtcct cgtccgggaa gtgacggtag accgtgagcc
      481 gggtgacgcc ggcccgttct gcgatgccgg caacggtcgt gtgggcgggt ccgacggtgc
      541 cgtggagatg gacggtggcc tcgatgatcc gttcccgggt cgcgtccatc tggtccgcgc
      601 ggcgcaccat gcgatagcca cgggatgatt tcgatgaacg catctgttca ctcaactctt
      661 gacaggccct ctcgcaacgt gtattcaatg aacactactg tacaacgaat atatctgagg
      721 agccggtcat ggcacagctc gacgcacctc ccctcaccgt tcgcaagccc ggtgaaggaa
      781 gagccggtgg cctcgtgccg ggactgggtg tgatcttcaa actcgacacc gccgacacca
      841 acggcatggt ctcggttgtc gagcacccct tcgacgtcgg cgtgttggtg ccgccgcaca
      901 tccatcaccg cgaggacgaa tattccatcg tcaccgaggg acagatcggc tttcgatccg
      961 gagaccgaga ggtcgtgctc ggcgccggcg gctacatcac caaaccgcgc ggcgaggtcc
     1021 acgcgatgtg gaacgccggg gctgtccccg cgcggatgat cgagatcatc agcccgggag
     1081 ggttcgaggg cttcttcacc gaactgtccg agctcgtggc ctccggccca ccgccggccg
     1141 aggcgcggct ggagatcgcg gaccgctacg gcctggagtt cgccgagccg tcgtggctgc
     1201 ccgagatcat cgagcgctac ggtctcactc cgccgccagg ctgacctcct cgcatttctg
     1261 ccccctggac caaccccttg atctcgacaa gctcgatcag cggactccgg cggtcgagct
     1321 tgtcgagacc ccccggaccc ttgatctcga cagctcgatc ggcggagtca gaagatgcgg
     1381 tggacggcgc cgaacagcat cgggccgatg aagctcacca acaccagcca cgcgacgatc
     1441 cggtgggaca gcttggccgg gttgagagag ccggccatcg ccaccagggc accctcttcg
     1501 gtgttgcggt ccagtcccat ccgggcggcg tcgtcgggga tgccgtgcct cgcacggaca
     1561 tggctggtca tctcgatgta tggctccggc tcgctcgccg accgctcgtc catgccgtga
     1621 tggtagcccg accaggcgca gattctcgcc gagcgacagc acctcattct cgccgagcga
     1681 cagcacctga ttctcgccga gcgacaatgc ccgttggagc ggtgatcttc gcggtgccag
     1741 ggtgtgagaa ggcatcgacg ctggcaccga cgaccggagg agccgcccat gcgagcagtc
     1801 cgcgttcacg cgtacggcaa ggacccgagc ctcgacgacg tacccgaacc caggatcgaa
     1861 ggacccctcg acgtcatcgt gcgcatcgac gcagcagggg tctgtcgcac cgacctgcac
     1921 atcatcgaag gccaatgggc agagaagtcc ggcgtgacgc tgccctacac gatcggacac
     1981 gagaacgccg ggacggtggc ggaagtcgga gaggcggtct cgaacgtctc ggtcgacgac
     2041 aaggtgatcc tgcacccgct cgtcacctgt gggctgtgcc gcgcgtgtcg ggccggcgac
     2101 gacgtccact gtacgaacag ccgcttcccc ggcatcaacg acgacggtgg gatggccgat
     2161 ctcctgcgca cgggtgcccg ttcggtcatc aggctggatg cggggctcga gcccaaagac
     2221 gtcgctgccc tcgccgacgc gggcctgacg gcgtaccacg cggtccgcaa ggcggtgccc
     2281 ctcctgtatc ccgggacgca cgtcgtgatg atcggcgccg gcggcctcgg ccacatcggg
     2341 ctccagtccc tgctcgcgct cacgcccgcc gaggtcaccg tcatcgaccg ctcgcccgac
     2401 gcgctgagga aggcgacgga gctcggcgcg caccacacgg tgaccgccgg tgacgacccc
     2461 gacgcccagg tctcggaggt gatggacgtg accggcggcc tcggtgctca tgtcgtgctc
     2521 gacttcgtcg gcgagcacgg caccgagtcg atcggggtgg cgatgacacg cgacgccggc
     2581 tcgtacttcg tgatcgggta cggcggtcac gtcgacatcg cgaccatcga catcatctcc
     2641 cgcgagatca acgtcatcgg caacctggtc ggctcctaca acgacct
//
LOCUS       DATBFZ010000552        11899 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_4906963, whole genome shotgun sequence.
ACCESSION   DATBFZ010000552 DATBFZ010000000
VERSION     DATBFZ010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 11899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11899
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4906963"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(9..932)
                     /locus_tag="VH419_16805"
     CDS             complement(9..932)
                     /locus_tag="VH419_16805"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HEY7045333.1"
                     /translation="MSGRSDRRCDVVVVGSVNRDYVCSSDRLPASGETVLGGEVTVGS
                     GGKGGNQAVSAAYWGATTAIVGCVGGDADGEALRADLEAAGVDTSALTVLSDVRTGCA
                     FVFVAPDGENAIVVAPGANGRVDPANVHAATRRLLRPGGVVVTQAEIPAPAAAAGVAA
                     AAEIGARAIVNLAPYRNTEAGLLQLADPLVVNESEAAGLLGSRIEDVAEARAAAQELA
                     RRTRSVVITLGPSGAVLADGVETLHVPAEERAAVDTTGAGDAFTGVVAAALAGGDDLA
                     TAVRWGVAAGTYAVGGHGAQASFPRRADIAG"
     gene            complement(929..1876)
                     /locus_tag="VH419_16810"
     CDS             complement(929..1876)
                     /locus_tag="VH419_16810"
                     /inference="COORDINATES: protein motif:HMM:NF013335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEY7045334.1"
                     /translation="MRRILLDTDLAMGAPGSDIDDGYALALALADPEITVDLVTTVGG
                     NSDVDTSTRLTHELLKRVDRTDVPVVRGAGAPLDPRWRRPGSAGRDTTSSLRARPSAA
                     AALVERVMAEPGELTIVAIGPLTNVALALMIEPRVADSVVEIVVMGGVYLKHTNVLGM
                     PGEYNFWCDPQAAASVLASGAPLRLVGLDVTEQVRLTRDHTAAMRAEPKRFAPYAAEC
                     ADGWIDHQQRVKAGDELEHGSCALHDPLAVAAVTHPELVTFQPAHVAVETASQLTRGV
                     AVADLLRWENPPPANCEIAVAVDADAFMRYFLDGMSSLP"
     gene            complement(2007..2681)
                     /locus_tag="VH419_16815"
     CDS             complement(2007..2681)
                     /locus_tag="VH419_16815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018022987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HEY7045335.1"
                     /translation="MTMQDASGRSAIVVRGGWEGHNPVEATDRFVPFLEEHGFSVEVR
                     DDLDVYTDAEKLAATDLVLQCYTQGTATDNQVNGLSAAVQAGTGLAGWHGGIVDSFRA
                     SPDYLHMTGGQWAAHPGGFVDYEVEVVPERADHPIVAGLTQWKHHTEQYWCLTDDLND
                     VLATSTFHVEDGMPWREQLVVPAVWTRQWGAGRVFVSTIGHKLEDLDVPEVRTLTERG
                     LLWASR"
     gene            complement(2678..4126)
                     /gene="xylB"
                     /locus_tag="VH419_16820"
     CDS             complement(2678..4126)
                     /gene="xylB"
                     /locus_tag="VH419_16820"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HEY7045336.1"
                     /translation="MPLVAGIDSSTQSCKVVICDSGTGRQVRQGVASHPDGTEVDPRA
                     WWDALQSAAKAAGGLDDVVAVAVGGQQHGMVCLDEHGEVVRPALLWNDTRSAQAADDL
                     IDELGGGDAGRTAWAEAVGLVPVASFTVTKLRWLAQHEPAAAARVAAVCLPHDWLTWQ
                     LAGARGLEALVTDRGDASGTGYWSAATGQYRRDLLQRALGHDVVLPTVLGPADAGGET
                     LAGARLAAGTGDNAAAALGLGAVAGDVMVSIGTSGVATAVSEVPVADPSGTVAGFADA
                     TGRYLPLVATLNAARVVDAVSYLLGRSQDEVAELALTVDPGADGLVLVPYLEGERTPN
                     RPDSTGAIHGLRLSTSTPAHLARAAIEGMLCGLADGVDALRDQGVEVRRLMLIGGGSR
                     SPAVRRIAPSVLGGEISVPAAGEYVARGAARQAAWVVAGTDEPPDWPLDGEECFTSEP
                     APFVRERYAQLRDLTARRASAHQPSQPSARRT"
     gene            complement(4170..5360)
                     /gene="xylA"
                     /locus_tag="VH419_16825"
     CDS             complement(4170..5360)
                     /gene="xylA"
                     /locus_tag="VH419_16825"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HEY7045337.1"
                     /translation="MTDPVARPEDKFSFGLWTVGWPARDPFGEPTRAPLDPVETVYRL
                     AELGAYGVSFHDDDLIPFGSDDTAREQHVSRFRQALESAGLVVPMMTTNLFTHPMFKD
                     GAFTSNDRGVRRYAIRKVMRNLDLAAELGAKTYVFWGGREGAEVDAAKDVRTALDRYR
                     EAFDVLGTYVKDNGYDIRFALEPKPNEPRGDILLPTVGHALAFISTLEHAEIVGLNPE
                     VGHEQMSNLNFVHAISQALWHGKLFHIDLNGQHGPKFDQDLVFGHGDLISAFGLVDLL
                     ENGGPGGGPAYDGPRHFDYKPLRTEDPEGVWQTAASNMRTYLLLKERTAAFRADPEVQ
                     EALAEAGVTELALPTLAEGESYADLLADPKSFESFDVEQAARRGYGFGRVDQLAIEHV
                     LGAR"
     gene            complement(5357..6625)
                     /locus_tag="VH419_16830"
     CDS             complement(5357..6625)
                     /locus_tag="VH419_16830"
                     /inference="COORDINATES: protein motif:HMM:NF012551.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HEY7045338.1"
                     /translation="MSQDLSPHDSHDPTSSGLSRRSLMIGAAAGVLGAAAGTVTTSAA
                     ARTSTLAATAPAPPLVPLWKTAGHRGILYGSSTATWQISDKAYSRLYTRHPAILFTED
                     DMLWYRLKPTPKSDLDFTYPDQIVEYAEDRNMLVFGAHLVWDEGFGDGWSDSDLWDIH
                     EKRARKLLYGTMRRLMHRYRGRVAIWSVVNEAIVNGPDVGFHGLRTDVPWYHTIGPEY
                     VAHAFHVAHEVDPRACLVMNDFGYETINQYGDKAVDKQRHTLQVLDRLLGRGVPVDAF
                     GIQAHLLAERFADRFHTRQYRRFLSELADRGLKILITEMDVLDDGLPRNPSRRDAMVA
                     DVYRRYLDCALDEPAVASLITFGLSDRYTWLQEDYPREDGAARRPLPFNAHLKPKPAF
                     HALHHALRQAPRRWPMFDAPRHVNTKDVPR"
     gene            6893..7858
                     /locus_tag="VH419_16835"
     CDS             6893..7858
                     /locus_tag="VH419_16835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HEY7045339.1"
                     /translation="MSSHRTPFDATTDPPTVTPDAMLFLGRPFTSAESSATGVSAMQL
                     RRLVREGTLRRLMAGVFVDAAAEDTPTLRAQALLCVLDRHVVVCDRSAAWLHGVDVLG
                     AEGQSVVPPLEVFRIEGHDGIRRPQCRRGKRTLGDSDVQVIRGIPVTTPLRTALDLGR
                     LARREEAIGAVDALMRIAGLSVEDLWKEMHRFRGARGVVQLRSLVGLANPLAESMAES
                     KLRIQLHDAGLPWPEAQWPVRHGRGFVMFRLDLAYPKLKLAVEYDGRDHHTSVADRTR
                     DDRRRAHLRRMGWTVVVLTARDVYGADARAARIVERELARLSKAA"
     gene            complement(7948..8169)
                     /locus_tag="VH419_16840"
     CDS             complement(7948..8169)
                     /locus_tag="VH419_16840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7045340.1"
                     /translation="MAKKRHSSASAEHLKQVAAGHDAPDYSHLPTEIDPDRLVETHDV
                     EPSVPEPRSVWATPDLADMARTNAIGGVV"
     gene            complement(8289..8645)
                     /locus_tag="VH419_16845"
     CDS             complement(8289..8645)
                     /locus_tag="VH419_16845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007433696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein RbpA"
                     /protein_id="HEY7045341.1"
                     /translation="MAGGSAIRGSRVGAGPMGEAERGDAAPRQRITYFCANGHETAPM
                     FAVDAQIPDTWDCPRCGLPANRDSDNPPPPPKIEPYKTHLAYVKERRSDAEAKEILDE
                     ALQTLRAKRKSGEIIF"
     gene            complement(8724..8957)
                     /gene="secG"
                     /locus_tag="VH419_16850"
     CDS             complement(8724..8957)
                     /gene="secG"
                     /locus_tag="VH419_16850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020384831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEY7045342.1"
                     /translation="MVLAFSILLVITSTLLVVMILLHKGRGGGLSDMFGGGVSSGLGG
                     SSVAEKNLDRITIGIALIWFACIVSLGLLMKNG"
     gene            complement(9015..9812)
                     /gene="tpiA"
                     /locus_tag="VH419_16855"
     CDS             complement(9015..9812)
                     /gene="tpiA"
                     /locus_tag="VH419_16855"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012920880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEY7045343.1"
                     /translation="MAAPTRIPLMAGNWKANLNHHEAVVLVQKLAWTLADKKHDYGRV
                     EVAVLPPYTDLRSVQTLVDGDRLKIGYGAQDVSVHDEGAYTGEITTGMLSKLGCSYVV
                     VGHSERREYHGEDDALVNAKAHKAVAAGLVPIVCIGEGLEIRQAGDHVAYTLGQLDGS
                     FAGFSAEQVAGLVVAYEPVWAIGTGEVATPEDAQEVCAAIRARVVEVHGDAAGGSVRI
                     LYGGSVKAANVAGIMAQPDVDGCLVGGASLQVDEFGGICRYYDMPVL"
     gene            complement(9812..11029)
                     /locus_tag="VH419_16860"
     CDS             complement(9812..11029)
                     /locus_tag="VH419_16860"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEY7045344.1"
                     /translation="MPGLDELAAAVGGFERKRVLVRSDLNVPLDGSTITDDGRIRASV
                     PTIGELRRRGARVVVMAHLGRPKGEPDPAYSLAPVAKRLGELLGGEVKQADDTVGESA
                     RAAVGAMSEGDVVLLENVRFNPGETSKDDGERGAFADRLAELGDAFVSDGFGVVHRRQ
                     ASVYDVAQRLPHAAGGLVLAEVDVLRRLTQDPERPYVVVLGGSKVSDKLGVIHNLLSK
                     ADRVLIGGGMVFTFLKAQGHEVGTSLLEAEQLGAVRGYLEQAESAGVDLVLPTDIVVA
                     PEVDAAAPTTVVPADAMPDDQLGLDIGPESAKRFASVVADARTVFWNGPMGVFEVAPF
                     AAGTKAVAEALTRVDGLSVVGGGDSAAAVRQLGFDDDQFGHISTGGGASLEYLEGKTL
                     PGLDVLRDHGQAG"
     gene            complement(11055..>11899)
                     /gene="gap"
                     /locus_tag="VH419_16865"
     CDS             complement(11055..>11899)
                     /gene="gap"
                     /locus_tag="VH419_16865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007077854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEY7045345.1"
                     /translation="LDADVEVTKDAIVVGGRSIAALEERDPSALPWKDLGADIVIEST
                     GFFTDATKAKAHIEGGAKKVIISAPAKNEDFTVVMGVNHEDYDPTTHDVISNASCTTN
                     CLAPMAKALNDEFGIVKGLMTTIHAYTQDQNLQDGPHKDLRRARAAALNIVPTSTGAA
                     KALGLVLPELKGKLDGYALRVPVPTGSATDLSFEAGRETSVEEVNAAMKAAAEGSLKG
                     FLSYTEDPIVSSDIVTDPASCIFDAGLTKVIGNQVKCVGWYDNEWGYSNRLVDLVNYV
                     GARL"
BASE COUNT         1803 a         4094 c         4149 g         1853 t
ORIGIN      
        1 accgccgctc aaccggcgat gtcggcgcgg cgcgggaagg acgcctgggc gccgtgcccg
       61 ccgacggcgt acgtcccggc ggcgaccccc caacgaacgg cggtggcgag atcgtcgcct
      121 ccggccagag cggccgcgac cacgccggtg aacgcatccc cggcgccggt cgtgtcgacg
      181 gcggcacgtt cctcggcggg tacgtgcagg gtctcgacgc cgtcggccag caccgcaccg
      241 gaggggccga gcgtgatgac caccgagcgg gtacgccggg cgagctcctg cgctgccgcc
      301 cgagcctcgg ccacgtcctc gatccggctg ccgagcaggc cggcagcctc gctctcgttc
      361 accaccagcg gatcggccag ctgcagcaac ccggcctcgg tgttccgata gggagccagg
      421 ttgacgatgg cgcgggcacc gatctctgct gccgcggcca ccccggcggc ggccgccggg
      481 gccgggatct ccgcctgcgt gaccaccacg ccgccggggc gaagcaaccg acgtgtcgcc
      541 gcgtgcacgt tcgccggatc cactcggcca ttcgcgccag gggcgaccac gatggcgttc
      601 tcaccgtccg gggcgacgaa gacgaacgca caccccgtcc ggacgtcaga caggaccgtc
      661 agcgcggacg tgtcgacacc cgcggcctcg aggtcggccc ggagcgcctc cccgtcggcg
      721 tccccgccca cacaccccac gatggcggtc gtcgcgcccc agtacgccgc cgagaccgcc
      781 tggttgccgc ccttgccgcc agagcccacg gtgacctcgc cgccgagcac ggtctccccc
      841 gacgccggga gtcgatcgct gctgcacacg tagtcacggt tgaccgaccc cacgacgacc
      901 acgtcgcatc ggcggtcgga gcggccgctc atggcaacga ggacatcccg tccaggaagt
      961 agcgcatgaa cgcgtccgcg tcgacggcga ccgcgatctc acagttcgcg ggtggcgggt
     1021 tctcccaccg cagcaggtcc gcgactgcga cacccctggt gagctgactc gcggtctcca
     1081 ccgcgacgtg cgccggctgg aacgtgacca gctcgggatg cgtcacggcc gcgaccgcga
     1141 gcgggtcgtg cagcgcgcag ctgccgtgct ccagctcgtc acctgccttg acccgttgct
     1201 ggtggtcgat ccagccgtcg gcgcactcgg cggcgtacgg cgcgaaccgc ttcggctccg
     1261 cccgcatcgc cgccgtgtgg tcacgcgtga gccgcacctg ctcggtcacg tcgagtccga
     1321 cgagccgcag cggggcaccg ctggccagca cggacgcagc ggcctgcgga tcgcaccaga
     1381 agttgtactc accgggcata ccgagcacgt tggtgtgctt caggtacacc ccgcccatca
     1441 cgacgatctc gacgactgag tcggccaccc gaggctcgat catcaacgca agcgcgacat
     1501 tggtgagtgg gccgatggcg acgatcgtga gctcgccagg ttcggccatg acgcgctcga
     1561 ccagcgccgc ggctgcggac ggccgcgccc gcaacgacga ggtggtgtcg cggccggcgg
     1621 atcccgggcg ccgccagcgc gggtccagcg gtgctccagc cccccggacg accgggacat
     1681 cggtacgatc cacccgcttc agcagctcgt gcgtgagccg ggtcgaggtg tcgacgtcgg
     1741 agttgccgcc gacggtcgtg acgagatcga cggtgatctc ggggtcggcc agcgccaaag
     1801 ccagcgcgta cccgtcgtcg atgtccgagc cgggcgcccc catggccagg tcggtgtcca
     1861 gcaggatccg cctcatacga gaaccacccg ccaccggctc ccgccgaccg cgaattgcgg
     1921 gaaagcgcag gtcgacacgt cgatctgaac gtgatgtttc ccgcaattgg cgggaagccc
     1981 aggccgcgga tggtgatcga acacggtcat cggctggccc agaggaggcc gcgctcggtg
     2041 agcgtcctga cctccggcac gtccaagtcc tcgagcttgt ggccgatggt cgagacgaac
     2101 acgcggccgg cgccccactg ccgggtccag accgctggga ccacgagctg ctcgcgccac
     2161 ggcatgccgt cctcgacgtg gaacgtggac gtcgcgagta cgtcgttcag gtcgtcggtc
     2221 agacaccagt actgctcggt gtggtgcttc cactgggtca acccggccac gatcgggtga
     2281 tcggcacgct cggggacgac ctccacctcg tagtcgacga agccgccggg gtgggccgcc
     2341 cactggccgc cggtcatgtg caggtagtcc ggtgaggcac ggaacgagtc cacgatgccc
     2401 ccgtgccaac cggcgagtcc ggttcccgcc tggacggcgg ctgacaggcc gttcacctgg
     2461 ttgtcggtcg ccgtgccctg ggtgtagcac tgcagcacga gatccgtcgc cgccagcttc
     2521 tccgcatcgg tgtagacgtc caggtcgtcg cggacctcga cgctgaagcc gtgttcctcg
     2581 aggaacggga cgaagcggtc ggtggcctcg accgggttgt gtccctccca cccgccgcgc
     2641 acaacgatgg cgctgcgacc tgatgcgtcc tgcatggtca tgtcctcctg gcggacggtt
     2701 gggacggctg gtgggcggaa gctctgcgcg cggtgagatc ccgcagctgg gcgtagcgtt
     2761 cccggacgaa cggtgccggc tcggaggtga agcactcctc accgtccagc ggccagtccg
     2821 gtggctcgtc ggtgccggcg accacccatg cggcctgacg agcggcacct cgggcgacgt
     2881 actcaccagc cgcgggcacg ctgatctcac caccgaggac gctgggggcg atccggcgta
     2941 ctgccggcga ccgcgagcca ccgccgatga gcatcaaccg acggacctcg acgccttggt
     3001 cacgcagcgc atcgacgcca tcggcgagtc cgcagagcat gccctcgatt gccgcccgcg
     3061 cgagatgtgc aggagtggag gtgctgagac ggagtccgtg gatggcgccc gtcgagtccg
     3121 gacggttggg tgtgcgttcg ccttcgaggt acggcaccag cacgaggccg tcggccccgg
     3181 ggtcgacggt cagcgccagc tctgcgacct cgtcctggct tcggccgagg agatatgaga
     3241 ctgcatcgac cacgcgggcc gcgttgagcg tcgcgacaag gggaaggtag cggccggtgg
     3301 cgtcggcgaa gcctgcgacg gtcccgctcg ggtcggcgac cggcacctct gacaccgcgg
     3361 tcgccacacc ggacgtgccg atggacacca tcacgtcgcc ggcgacggcg ccgaggccca
     3421 acgcagctgc ggcgttgtcg ccggtccctg ctgcgagccg ggcacctgcc agtgtctcgc
     3481 cgccggcgtc tgccggaccc aggacggtcg ggagtacgac gtcgtgaccg agcgcgcgtt
     3541 gcaacaggtc acggcggtac tgccctgttg cagcggacca atagccggtg ccgctggcat
     3601 caccccgatc ggtgacgagc gcctcgagac cacgagcgcc ggcgagttgc caggtcagcc
     3661 agtcatgcgg caggcagacg gcggccaccc gtgccgcggc ggcgggctcg tgctgggcca
     3721 gccagcgcag cttggtgacc gtgaaggacg cgacggggac gaggccgacc gcctcggccc
     3781 aggcggtcct ccctgcgtcg ccaccgccga gctcgtcgat cagatcgtcg gccgcctgcg
     3841 cggagcgggt gtcgttccac agcagcgcgg ggcggacgac ctcgccgtgc tcgtcgagac
     3901 agaccatgcc gtgctgctgc ccgccgactg cgaccgccac gacgtcgtcc agccctccgg
     3961 cggcctttgc cgccgactgc aaggcgtccc accaggcgcg aggatcgacc tcagtgccgt
     4021 cggggtgcga agcgacgccc tgccggacct gtcggccggt gcccgagtcg cagatgacga
     4081 ccttgcagga ctgggtggac gagtcgatgc ctgcgacgag cggcatgcgg cgaggtcctc
     4141 tctgtctgcg ggtggtgggg ctgacgcgat cagcgagcgc cgagcacgtg ctcgatcgcg
     4201 agctggtcga cgcggccgaa cccgtagccg cgacgcgcgg cctgctcgac gtcgaagctc
     4261 tcgaacgact tcgggtcggc cagcaggtcg gcgtagctct cgccctcggc caacgtcggc
     4321 agcgccagct cggtgacacc ggcctcggcg agcgcctcct ggacttcggg atcggcgcgg
     4381 aacgcggctg tccgctcctt caggagcagg taggtccgca tgttggatgc cgcggtctgc
     4441 cacacccctt cggggtcctc ggtgcgcaac ggcttgtagt cgaagtggcg tggaccgtcg
     4501 tacgccggtc cgcccccggg tccaccgttc tcgagcaggt cgaccaggcc gaacgcgctg
     4561 atcaggtccc cgtgtccgaa gaccaggtcc tggtcgaact tcgggccgtg ctggccgttg
     4621 aggtcgatgt ggaagagctt cccgtgccac agcgcctggg agatcgcgtg cacgaagttg
     4681 aggttggaca tctgctcgtg cccgacctcg gggttgagcc cgacgatctc ggcgtgctcc
     4741 agggtggaga tgaacgcgag cgcatgcccg acggtcggca gcaggatgtc accacgcggc
     4801 tcgttcggct tcggctccaa cgcgaaccgg atgtcgtagc cgttgtcctt gacgtacgtg
     4861 ccgagcacgt cgaaggcctc ccggtagcgg tcgagagcgg tccggacatc cttggcggcg
     4921 tcgacctcgg cgccttcccg gccaccccag aacacgtacg tcttcgcgcc cagctcggcc
     4981 gccaggtcga ggttgcgcat caccttgcgg atggcgtagc ggcgtacgcc ccggtcgtta
     5041 ctggtgaacg ccccgtcctt gaacatcggg tgggtgaaga ggttcgtggt catcatcggc
     5101 acgacgagac cggccgactc cagtgcctgc ctgaaccgcg acacgtgctg ctcgcgagcg
     5161 gtgtcgtccg agccgaaggg gatgaggtcg tcgtcgtgga agctgacgcc gtaggcgccg
     5221 agctcggcaa gccggtacac ggtctcgacc gggtcgagcg gcgcccgggt gggctcgccg
     5281 aacgggtcac gggccggcca acccaccgtc cacaggccga aggagaactt gtcctccggc
     5341 cgggcgacag gatcggtcat cgtggcacgt ccttggtgtt cacatgacgc ggtgcgtcga
     5401 acatcggcca gcggcgtggt gcctggcgca acgcgtggtg gagcgcgtgg aacgccggct
     5461 tcggcttcag gtgggcgttg aacggcagcg gtcggcgtgc tgccccatcc tcccgcgggt
     5521 agtcctcctg caaccaggtg tatcggtcgc tgagaccgaa cgtgatgagc gacgcgacgg
     5581 ccggctcgtc gagggcgcag tcgaggtagc gtcggtagac gtcggcgacc atcgcgtctc
     5641 gcctgctcgg gttcctcggc agcccgtcgt cgagcacgtc catctcggtg atgaggatct
     5701 tcagtccacg gtcggcgagc tccgagagga accgccggta ctggcgggtg tggaaccggt
     5761 cggcgaaccg ttcggcgagc aggtgcgcct ggatgccgaa ggcgtcgacc ggtacgccgc
     5821 ggccgagcaa ccggtcgagg acctgcaggg tgtgcctttg cttgtcgacg gccttgtcgc
     5881 cgtactggtt gatcgtctcg tagccgaagt cgttcatcac caggcaggcg cgcgggtcga
     5941 cctcgtgggc gacgtggaag gcatgggcga cgtactccgg tccgatcgtg tggtaccagg
     6001 gcacgtcggt gcgcaggccg tggaagccga cgtccgggcc gttgacgatg gcttcgttga
     6061 cgaccgacca gatcgcgacc cggccgcgat agcggtgcat gagccggcgc atcgtgccgt
     6121 agaggagctt gcgggcccgc ttctcgtgga tgtcccagag gtcgctgtcg ctccagccgt
     6181 cgccgaagcc ctcgtcccag acgaggtggg cgccgaagac gagcatgttg cggtcctcgg
     6241 cgtactcgac gatctggtcg gggtaggtga agtcgaggtc ggacttcggg gtcggcttga
     6301 gccggtacca gagcatgtcg tcctcggtga agaggatcgc cgggtggcgg gtgtagaggc
     6361 ggctgtacgc cttgtcggag atctgccagg tcgcggtcga ggagccgtag aggatcccgc
     6421 ggtggccggc ggtcttccac aacggcacga gcggcggcgc cggagctgtt gccgcgaggg
     6481 tggaggtgcg cgcggcggcg gacgtcgtga cggtgccggc cgcggcgccg agcacgcccg
     6541 cggcagcccc gatcatcagc gaccgacggg acagacccga actcgtgggg tcgtgagaat
     6601 cgtgcgggct caggtcctgg ctcatgcgtc gcgctcctgt gagaggtccg gtgcaaatgt
     6661 gagaggtccg gtgcaaaacg attgcgtcaa tcggtacgac cggagagtaa gccctcttcc
     6721 cggagcccgg caagggtttc ccaccccccg ccgaggtggc aagtttcgtt caactttgtc
     6781 ggcgtgtcgt gcggcctgtg ccacctcggt ccggcaagcc gccgcgcgag aggggttcag
     6841 gcctgtggac agcgtgcgcg aggctgccct ccactggtgc acgctgtcgg ccatgtcctc
     6901 gcaccgtacg ccgttcgacg ccaccacaga cccgccaacc gtgacgccgg acgccatgct
     6961 gttcctcggt cggccgttca ccagtgctga gtcgtcggcg accggcgtct ccgcgatgca
     7021 actgcgtcgt ctggtccgag aagggacgtt gcggcgactg atggcgggag tcttcgtcga
     7081 cgcggctgcg gaggacacgc ccacacttcg cgcgcaggcg ctgctctgcg tgctcgaccg
     7141 gcacgtcgtc gtgtgcgacc gctcggcggc ctggctgcat ggcgtggacg tcctgggtgc
     7201 ggagggacaa agcgtcgtac cgcctctcga ggtgttccgc atcgaaggcc atgacgggat
     7261 ccggcgaccc cagtgccgac gaggcaaacg gacgttgggc gactccgacg tccaggtcat
     7321 ccgggggatc ccggtcacga ctccgctccg gactgcgctc gacctcggcc ggttggcccg
     7381 gcgggaggag gcgatcggcg ctgtggacgc gctgatgcgg atcgctggcc tgtcagttga
     7441 ggacctgtgg aaagagatgc accggttcag gggcgcgcgg ggcgtcgtac agctccgcag
     7501 ccttgtcggg ttggcgaacc cgttggcgga gtcgatggcc gagtccaagc tgcggatcca
     7561 gctgcacgac gcgggactgc catggccgga ggcgcagtgg ccggtgcgcc atggccgagg
     7621 gttcgtgatg ttccgcttgg acctcgccta cccgaagctc aagctggcgg tcgagtacga
     7681 cggccgcgac caccacacct cagtcgcgga tcgcacccgc gacgaccgtc ggcgagccca
     7741 ccttcgccgg atggggtgga cggtggtggt cctcaccgcc cgagacgtct acggcgccga
     7801 tgctcgcgcc gcccgcatcg tcgaacgcga actggctcgc ctctcgaagg ccgcctgagt
     7861 ggccacccgg atcgaagtgg caatcgcggt ccgacacgcc gtcaaagtcc aacgaaagct
     7921 gccacctcgg cgttggcgag ggcgccgcta gacgacgccg ccgatggcgt tggtccgggc
     7981 catgtcggcc aggtccggcg tggcccacac ggagcggggt tcaggcaccg agggctcgac
     8041 gtcgtgcgtc tccacgagtc gatcggggtc gatctcggtg ggcagatgcg agtagtccgg
     8101 agcgtcgtga ccggctgcga cctgcttgag gtgctcagcg ctggcgctcg aatggcgctt
     8161 cttcgccata catcgagggt acgacggcgg accaagccca cgatcgaggt ggcaatcgcg
     8221 gtccgacacg ccggcaaatt ccaacgaaac ttgccacctc ggcgggagat agggagaggg
     8281 agagggggtc agaagatgat ctcgccggac ttgcgtttgg cgcgaagggt ctgcagcgcc
     8341 tcgtcgagga tctccttggc ctcggcgtcg gagcggcgct ccttcacgta ggccagatgc
     8401 gtcttgtacg gctcgatctt cggcggcggc ggcgggttgt cggagtcgcg gttcgcgggc
     8461 agaccgcacc gtgggcagtc ccaggtgtcc gggatctggg cgtcgacggc gaacatgggc
     8521 gccgtctcgt gcccgttggc gcagaagtag gtgatccgct gccgaggcgc cgcatcgcca
     8581 cgctcggcct cgcccatcgg acctgcaccc acacggctgc cccggatcgc gctgccacca
     8641 gccacatcaa actcctcgac tcagggtgtc gatgcctcgc ctgccagcct ggcatcaggt
     8701 caggtgattg tacgacgcac ggctcagccg ttcttcatca acagaccgag cgagacgatg
     8761 caggcaaacc agatgagcgc gataccgatc gtgatccggt ccaggttctt ctccgccact
     8821 gacgaaccgc cgagcccact ggacacgccg ccgccgaaca tgtcggacaa gccaccgccg
     8881 cgtcccttgt gcagcaggat catgacgacg aggagtgtgc tcgtgatgac gagcaggatg
     8941 ctgaaagcca agaccacggg cagagagtct acctgccggt gtcgcgcttc agagaatcgg
     9001 cggttcgacc cacctcagag gacgggcatg tcgtagtacc ggcagatacc gccgaactcg
     9061 tcgacctgca aggacgcgcc gccgaccaga cagccgtcga cgtcgggctg cgccatgatc
     9121 ccggccacgt tcgccgcctt gaccgagccg ccgtacagga tgcgcaccga gccaccggcg
     9181 gcgtcgccgt gcacctcgac cacccgcgca cgaatcgcag cacacacctc ctgggcgtcc
     9241 tccggcgtgg cgacctcgcc ggtgccgatc gcccacaccg gctcgtacgc gacgaccagg
     9301 cccgcgacct gctcggcgga gaatcctgcg aaggacccgt cgagctggcc gagcgtgtac
     9361 gcgacgtggt caccggcctg gcggatctcg agcccctcgc cgatgcagac gatcgggacg
     9421 agacctgcgg caaccgcctt gtgcgccttc gcgttgacga gcgcgtcgtc ctcaccgtgg
     9481 tactcacgac gctcggagtg gccgacgacc acgtacgagc accccagctt cgacagcatc
     9541 cccgtcgtga tctcgccggt gtaggcgcct tcgtcgtgca cggacacgtc ctgcgcgccg
     9601 tacccgatct tcaaccggtc gccgtcgacg agcgtctgca cgctgcgcag gtcggtgtac
     9661 ggcggcagca ccgccacctc gacccgaccg tagtcgtgct tcttgtcagc cagcgtccag
     9721 gcgagcttct gcacgagcac gactgcctcg tggtggttga ggttggcctt ccagttgccg
     9781 gccatcaacg ggatgcgggt gggagctgcc atcagcccgc ctgcccgtgg tcgcgcagca
     9841 cgtccaggcc gggcagcgtc ttgccttcga gatactccag gctcgcgcca ccaccggtgg
     9901 agatgtggcc gaactgatcg tcgtcgaagc cgagctgccg tacggcggcg gccgagtcac
     9961 caccgccgac gaccgagaga ccgtcgacgc gcgtcaatgc ctcggcgacc gctttcgtgc
    10021 cggcagcgaa cggcgccact tcgaagacac ccatcggacc gttccagaag accgtcctgg
    10081 cgtcggcgac aacggatgcg aaacgcttcg cagactccgg gccgatgtcg agaccgagct
    10141 ggtcgtccgg catggcatct gccggcacga ccgtggtggg tgctgcggcg tcgacctccg
    10201 gcgccacgac gatgtcggtc gggagcacca ggtcgacgcc cgcggactcg gcctgctcga
    10261 gatagccgcg aaccgcaccg agctgctcgg cctcgagcag gctggtgccg acctcgtggc
    10321 cctgcgcctt gaggaaggtg aagaccatcc cgccgccgat gaggacacgg tcggccttcg
    10381 acagcaggtt gtggatgact ccgagcttgt ccgacacctt cgagccaccg agcacgacga
    10441 cgtacggtcg ctcggggtcc tgcgtcagcc gtcgcaggac gtccacctcc gcaagcacga
    10501 gcccgcctgc cgcgtgcggg agacgctgag ccacgtcgta cacgctggcc tgcctgcggt
    10561 ggacgacgcc gaatccgtcg ctgacgaacg cgtccccgag ctccgcgagc cggtcggcga
    10621 acgcaccgcg ctcgccgtcg tccttgctcg tctcgcccgg gttgaagcgg acgttctcga
    10681 gcaggacgac atcgccctcg gacatcgcac cgaccgctgc ccgtgcggac tcccctaccg
    10741 tgtcgtcggc ctgcttgacc tccccgccga gcagctcacc gagccgcttc gcgactggag
    10801 cgagcgagta cgccggatcc ggctcaccct tggggcgacc gagatgcgcc atcacgacca
    10861 cccgggcccc ccggcgtcgt agctcgccga tcgtggggac gcttgcccgg atgcgtccgt
    10921 cgtcggtgat cgtggacccg tcgagcggga cgttcaggtc ggaccggacg aggacgcgct
    10981 tgcgctcgaa cccgccgacc gccgcggcga gctcgtcgag cccgggcagc gtcgagggca
    11041 cggtggcgac ccggtcagag ccgggcgccg acgtagttga ccaggtcgac aagccggttg
    11101 gagtagcccc attcgttgtc gtaccagccc acgcacttca cctggttgcc gatcaccttg
    11161 gtcagaccgg cgtcgaagat gcaggaagcg gggtcggtca cgatgtcgct cgacacgatc
    11221 gggtcctcgg tgtaggagag gaagcccttc agcgacccct ccgcggctgc cttcatcgcc
    11281 gcgttgacct cttcgaccga ggtctcccgg ccggcttcga acgacaggtc ggtggccgag
    11341 ccggtcggca ccggcacccg caaggcatac ccgtcgagct tgcccttgag ctcggggagc
    11401 acgagaccga gtgccttggc ggcaccggtc gacgtcggca cgatgttgag cgccgccgcc
    11461 ctggcccggc ggaggtcctt gtgcggacca tcctgcaggt tctggtcctg ggtgtaggcg
    11521 tggatcgtgg tcatcagccc cttgacgatc ccgaactcgt cgttgagtgc cttcgccatc
    11581 ggcgccagac agttcgtcgt acacgaggcg ttggagatga cgtcgtgcgt ggtggggtcg
    11641 tagtcctcgt ggttgacacc catcacgacc gtgaagtcct cgttcttcgc cggcgcggag
    11701 atgatgacct tcttcgcgcc accttcgatg tgcgccttcg ccttggtcgc gtcggtgaag
    11761 aaccccgtcg actcgatgac gatgtcggcg ccgaggtcct tccacggcag tgccgacggg
    11821 tcgcgctcct cgagggcggc gatgctgcgg cccccgacga cgatcgcatc cttggtgact
    11881 tcgacgtcgg cgtcgagcc
//
LOCUS       DATBFZ010000553        13173 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_6218093, whole genome shotgun sequence.
ACCESSION   DATBFZ010000553 DATBFZ010000000
VERSION     DATBFZ010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 13173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13173
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6218093"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="VH419_16870"
     CDS             complement(<1..434)
                     /locus_tag="VH419_16870"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY7045346.1"
                     /translation="MSDTEAATPLRTTIPARLDRLNWTPFHTRMVAGLGAAWILDGLQ
                     ITISSAVTGQLTKPATLGMSSTEVGLIASVYLVGEMVGALYFGRQSDRLGRKRLLILT
                     LLLYLVGTGAAAFTTGHHHGWLLYFYGTRFVAGMGIGGQYAA"
     gene            complement(431..1648)
                     /locus_tag="VH419_16875"
     CDS             complement(431..1648)
                     /locus_tag="VH419_16875"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY7045347.1"
                     /translation="MTARAESRVVNLAGLAQGVTLVTFPAASSVLTDPDEYDLSSTQY
                     GAMFVPQVLTAIAAALLGTALARHITLKRVLVLGLGANIASMLLLLLSTLVKSQTSIA
                     YPLLLVATACLGVGFGLTVPALNTYVALFRPTAADRAVLMLNALLGLGTALAPVFVAV
                     FVGLGFWWGMPVLSAVALTAIGAGCLQLPLKASTPAAGAAQARSAPIPGRFWFFAGFA
                     LLYGMAETMNGNWSQLDLSDLGASTTVAAVGLTAFWAMVTVGRLAFAAAERWIPSRVT
                     YHSLPVALAVVFIVIASLPDDTPWLAVVAFGLAGLGCSALLPLTISFGHDALPEMADG
                     VAGGVIAFYQIGYGIVAFGVGTLVDNGRSLSSLYGLMAFVAAGMAVLSFTVTRDRPSL
                     VASSPSAVSEDTS"
     gene            complement(1651..3228)
                     /locus_tag="VH419_16880"
     CDS             complement(1651..3228)
                     /locus_tag="VH419_16880"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEY7045348.1"
                     /translation="MSAEYDVIIVGSGAGGGTLAHRLAPSGHRILILERGDWLPREIE
                     NWDAQAVFVDNRYVSKDTWYDATGQPFQPQIHYFVGGATKFYGAALYRLRELDFGELK
                     HHDGISPAWPIGYDVMEPYYTQAERLYCVHGNRGEDPSDPPASEPYPYPAVSHEPRMQ
                     RLFDDMAKVGLHPFHSPSGIMLDESRPELSRCIRCATCDGFPCLVHAKSDADVVAVRP
                     ALDHANVTLVRNAHVRHLETDASGGAVTAVVAEVNGEPQRFTGAIVVLSAGAANSAKL
                     LLASANDRHPNGLGNGSDQVGRNYLFHNSRAFLAVSTEKNPTKFQKTVGVNDYYFGDD
                     DFEYPMGNVQMVGKSSAPMYRGEKPIEAGLAPTFALNDVADHAVDFWLSTEDLPDPDN
                     RVTLNRDGDIVLSYTPNNRKPLEQLYHRVKKHLSHLGMHPHHLIPRDIYMKNDIPVAG
                     VAHQAGTARFGNDPATSVLDTNCKAHELDNLYVVDTSFFPSIGAVNPALTAMANALRV
                     GDHLVERLGRPGSAAES"
     gene            complement(3433..5571)
                     /locus_tag="VH419_16885"
     CDS             complement(3433..5571)
                     /locus_tag="VH419_16885"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEY7045349.1"
                     /translation="MVTRTALVDRMVAADEPVITVVAPAGYGKTTLLAQWARRRTPRV
                     VWVSCDVADNDPVTLWTALLTGLDAVEPVGPAAARILAAYGGGVDAVPGLASAVGSLS
                     EPVTVILDHVETITAPACRAALAEFALRVPVGWQLAMSSRDQVPIRAARMRTQGEVLE
                     LSASDLAMAGAEARALLASAGVDLSKAQADALVGTTEGWPVALYLAVLAMKSGAPAEN
                     FTGEDWLVGDYLATELLPSLTKTQAGFLQRTSILDRMSGPLCDAVLDSTGSARLLERL
                     EKRNLLVVPLDRRRRWYRYHHLLREHLYAELRADRTAEIPSLHARASAWYENNDLPEK
                     AIEHAQAAGDADRVAALVLQVMQPVWVSGRVDTVAGWMEWLGERPPTQHSAAITAHGS
                     LIFALLGRASEAERWTAIAERLPAEGTLPDGSTVAGTMAYLRANLGRDGPAAMRRDAG
                     AALTGLSPDSPYRATMMYVQGISHLLEGHLDQADAMLAHACDLATELAVGPLTALTLA
                     ERYLVAAERGDWIAADSYARRSLEIVDDGHFDGYWTSALVLAIAARSCARSGDIPSAR
                     RLIRRAARLRRLLTYALPVVSVQALIQLANAYIAVVDPGGARAAIEQATAILQQRPHL
                     GNLAGEVEHLRSRVERISGSGTGASSLTAAELRLLPLLPTHLSLPEIGARLFISRHTV
                     KSEVSSIYRKLGVSSRTAAVERISELGLHG"
     gene            complement(5565..5873)
                     /locus_tag="VH419_16890"
     CDS             complement(5565..5873)
                     /locus_tag="VH419_16890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7045350.1"
                     /translation="MNQDGHDDLPVPVISLVITLVISLVISLVISLVIALVIARGRNA
                     FLSVPHRATFVPCLLGTVATRFGHFGPTPNRRQRPVARRALKAVLVRSSSCLWRVDGW
                     "
     gene            complement(5870..7522)
                     /locus_tag="VH419_16895"
     CDS             complement(5870..7522)
                     /locus_tag="VH419_16895"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY7045351.1"
                     /translation="MEDTRAEDPIEPPSEVATAEAAEAPPRAGLILTALILVAGVANL
                     NLSVANVALPSIGLAFDASQTSLNLIAIGYSLGLAGSVLYLGAVGDRYGRKLLLLLGV
                     TLSIPACLLAAYAPNEYVLAGARIFGGVSAGMAYPTTLALITALWAGQPRTKSIALWS
                     ALGGAISALGPLVAGAMLEHFWWGSVFLVTLPLAGVALVMAWLLVPAHVNEDSDPVDN
                     LGGILSVVLVGAVVLAINLAPVPGKLSLALGLALIAVAAVGAFLIRQRRAANPLYDLR
                     VASRRIFWVAATAGIIVFGSLMAAAFVSQQYLQNVLGYSTLEAGAAFLPAIVMMVLVA
                     PRSAKLIESHGSRLTLLLGYCFLLLAFLSMLLLWKEGSSYWQIGIAYAFIGVGVGLAG
                     TPASHSLTGSVPRARAGMASGTADLQRDLGGAIMQSILGALLTTGYASAFAAQITSSP
                     DADQVSKNVQSELTKSFSSAEETAKRYPQYADQITAAAKTSFLDGDDWAYGAGAVAVV
                     LGATLVFFCFPKQREEEELLSRYRLEDTQPGGRDRTTQSPAQ"
     gene            complement(7551..8927)
                     /gene="zwf"
                     /locus_tag="VH419_16900"
     CDS             complement(7551..8927)
                     /gene="zwf"
                     /locus_tag="VH419_16900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020580229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEY7045352.1"
                     /translation="MTTTQPVDALVIFGATGDLAKLETFPALVGLVERGLLDVPVIGV
                     AKSGWGLEDFRNYAADSLRRNDLDPDSGSGKRMLGLLEYVDGDLADAATYQTLADKLP
                     DGARVLFYLEVPPPLFGTIADGLGKAGLTDGARVMVEKPFGTDLESARKLNETMHKVF
                     SEDAIFRVDHWLGLDPVENVLFTRFANTLFEAVMNRTYVENIQITMAEAFDVADRGAF
                     YDRTGAIRDVLQNHMLQVLAAVTIDPPQGGLGGVRDVRAGLVKAIRPLTPDDVVRGQY
                     EGYLDVEGVAPGSTTESFVAVRLAVDSWRWEGVPFLIRAGKCMPVTATEITVNFRRPP
                     QNVFGLDPFEGMNYLRFRIWPETQVGLTVAGKKPGAEMVPQTNDVTFIERPNTEMRPY
                     DRLIGAALADEQWLFARQDTVEAAWRVVGPVLGDVTPLHTYARGTWGPSEADSLLEPG
                     ESWRALEA"
     gene            complement(8928..10763)
                     /locus_tag="VH419_16905"
     CDS             complement(8928..10763)
                     /locus_tag="VH419_16905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020576182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEY7045353.1"
                     /translation="MEHYPPIAEHGLIGDLQTSALVTTDGTIDWLCLPRFDSPSVFGS
                     LLDREKGGELTITPAAETYTTKQLYIPDTAILLTRFLTADGVGEITDFMIPLPGQPAT
                     DNHRLVRRVRCVRGSMEFKGLLKPRFDHGRRPHKTSVVEPGVVLDDGELSLTMHVVRE
                     PEDEHLIRPTLDADGDIAISLTLQAGQVRGIVLESAAEGPPREIRVAEFQQMFDETVA
                     YWRDWLDQSTYTGRWREAVHRSAVALKLMTYAPSGALVAAPTAGLPEQLGGERNWDYR
                     YTWVRDASFSVYALLGLGFTDEAMGFLTWLRDRIRGDSASGRPLDIMYRVDGSSDLVE
                     ESLDDWDGYQHSRPVRIGNGAATQVQMDIFGEAMDSVFFASEHGIDVGHHGWLAITEM
                     IDWLCDNWDQPGEGIWESRGGQQDFTYGRVMSWVAFDRAIRMAGSMGRPADINRWVQA
                     RDAIYAQVMDKGWSEKREAFIQHYGSDVLDSSLLRMATVGFIAPTDPMWASTLNAMEQ
                     ELVTDSLVYRYDPAASPDGLTGDEGTFSLCTFNYVDALARAGRLEEARLTFDKMLTYA
                     NHLGLFSEEIALTGEQLGNFPQAFTHLALIDAAITLDRALNRGRQ"
     gene            complement(10878..11909)
                     /locus_tag="VH419_16910"
     CDS             complement(10878..11909)
                     /locus_tag="VH419_16910"
                     /inference="COORDINATES: protein motif:HMM:NF014386.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEY7045354.1"
                     /translation="MSDVAAVLLFAAVTIYAVFGGADFGAGFWDLVAGGAHRGDRPRE
                     VIDHAVAPVWEANHVWLVFTFVVLWTCFPEAFESITLTMFVPLTLAALGIVLRGASFA
                     FRKAVFSTRERRNFGAVFAVSSVLVPYCLGAVAGAVAAGRIPAGGEAGDPWSSWVNAT
                     SVLAGVLAVSLAAYLAAVFLVWDARRLSDEVMVAYFRRRAVAAAVVAGVVASAGLFVL
                     HDEATYVFDGLMSRGLPLAILSVIAGCAALVLLRRDSHVVARPAAVLAVVALVVGWGV
                     AQWDYLLPTTLTISDAAAPDGTITAVLVAAALAAVLIVPAFVLLYVLDQRSLLPEEGL
                     EEPVGAEET"
     gene            complement(11906..>13173)
                     /locus_tag="VH419_16915"
     CDS             complement(11906..>13173)
                     /locus_tag="VH419_16915"
                     /inference="COORDINATES: protein motif:HMM:NF013791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEY7045355.1"
                     /translation="KHDDADALLLAQRWSKYMAVTFAVGAVTGTVLTFEFGLLWPKFM
                     GQWGQAFGIPFAFEGIFFFAEAVFISIYIFGWRRLRPWAHFWTGVPIPVAGVFGSISI
                     VAANAWMNSPQGVTLDASGNIVDVDPVQVILNDAMPLMAAHMVIAAYLVGGFLIASVY
                     AVGMLRGRRDRYHRLGFIIGFTVAAIATPVQMGVGDTLARWVYDNQPVKFAAIEMVPE
                     TSSDVPETLLGHLNSEGEVTGGIPIPGLASWLSDPSEGKSTVVQGLDTVPSSERPSIH
                     EVNVVHLAWDVMVGVGTLLFLLTLWYWASWIFRRDIPKSRWFLRCCAVAGALSVVTLE
                     AGWTVSEVGRQPWIVYEKMKVEDAATANTGVWITFVAVIILYAALGTTIILVLRAMSR
                     RFRQQRGFDDRDAPYGPSDVTVEPEVPVG"
BASE COUNT         2330 a         4483 c         4322 g         2038 t
ORIGIN      
        1 atggccgcat actggccacc aatccccatc ccggcgacga aacgggtccc gtagaagtac
       61 aacaaccagc cgtgatgatg gccggtcgtg aacgccgccg cccccgtacc gaccaggtag
      121 aggaggagcg tgaggatcag caggcgcttg cgaccgagcc tgtcggactg ccgtccgaaa
      181 tagagggctc cgaccatctc gccgaccagg tacaccgagg cgatgagtcc gacctcggtc
      241 gagctcatgc cgagtgtcgc cggtttggtc agctgacctg tcacggcgct cgagatcgtg
      301 atctgcaggc cgtcgagaat ccatgctgcg cccagtccgg cgaccatacg ggtatggaag
      361 ggtgtccagt tcaaccggtc gaggcgggcc ggaatcgtcg tgcgcagggg cgttgccgcc
      421 tcggtgtcgc tcatgacgtg tcctcactga ctgccgaagg ggaggatgcg acgagggagg
      481 gtcggtcgcg ggtcacggtg aacgacaaca ccgccatgcc agcagcgacg aacgccatca
      541 atccgtagag cgatgaaagg ctccggccgt tgtcgacaag ggtcccgacg ccgaacgcaa
      601 cgatcccgta gccgatctgg tagaaggcga tgaccccgcc tgccacgcca tcagccatct
      661 ccggcagagc gtcgtggccg aagctgatgg tgagcggcag cagggcggag cagccgagcc
      721 ccgcgagccc gaacgcgaca acggcgagcc acggtgtgtc gtccggcagg ctcgcgatca
      781 cgatgaacac gacggcaagc gcgactggga gcgagtggta ggtgacccga gaaggaatcc
      841 accgctccgc ggctgcgaac gcgagccgac cgaccgtcac catcgcccag aaggcggtca
      901 gcccgactgc ggccaccgtc gtcgaagcgc cgagatccga caggtcgagc tgcgaccagt
      961 tgccgttcat cgtctctgcc attccgtaca gcagcgcaaa gcccgcgaag aaccagaacc
     1021 gcccgggaat cggggccgat cgtgcctgtg ctgcgccggc ggccggcgtg ctcgccttca
     1081 acggaagctg cagacagccc gcgccgatcg ccgtcagcgc aaccgccgac agcaccggca
     1141 tgccccacca gaatcccagc ccgacgaaca cggccacgaa caccggtgcc agcgcggtgc
     1201 cgaggccgag gagcgcgttg agcatcagca cggcacgatc tgcggccgtt ggccggaaca
     1261 gcgccacata ggtgttcagt gccgggacgg tcaatccgaa accgacgcca agacatgccg
     1321 tcgccaccag cagcagcggg taggcgatgc tcgtctggga cttcacgagc gtgctcaaca
     1381 gcagcagcag catcgaggcg atgttcgcgc cgagcccgag gaccagcaca cgcttgagtg
     1441 tgatgtgcct cgcgagggcg gtcccgagca gcgcggcggc gatcgctgtc agtacctgcg
     1501 gcacgaacat ggcgccgtac tgcgtgctcg acaggtcgta ttcgtcggga tcggtcaaca
     1561 cggagctggc agcaggaaac gtcaccagcg tcacgccctg tgccaggcct gccaggttga
     1621 ccacccggct ctcagcccgg gccgtcatca tcacgactcc gcggccgacc cgggccttcc
     1681 aagtcgttcg acgaggtggt cgccgacacg aagcgcgttc gccatggcgg tcagtgcggg
     1741 gttcaccgca ccgatgctgg ggaagaagct cgtgtcgacg acgtacagat tgtcgagctc
     1801 atgggccttg cagttcgtgt cgagtaccga ggtggccgga tcgttgccga accgcgcggt
     1861 gcccgcctgg tgtgcgacac cggcgaccgg aatgtcgttc ttcatgtaga tgtcgcgcgg
     1921 aatcaggtgg tgcggatgca tgcccaggtg actgagatgc ttcttgaccc ggtggtacag
     1981 ctgctcgagc ggcttgcggt tgttgggcgt gtagctcaac acgatgtcac cgtcgcgatt
     2041 gagcgtcacg cggttgtccg ggtcgggcag atcctccgtc gacagccaga agtccaccgc
     2101 gtgatcggcg acatcgttga gtgcgaaggt cggagcgagc ccggcctcga tgggcttctc
     2161 gccccgatac atcggagcgg atgacttccc gaccatctgg acgttgccca tcgggtactc
     2221 gaagtcgtcg tcaccgaagt agtagtcgtt cacgcccacg gtcttctgga acttcgtcgg
     2281 gttcttctcc gtcgagacgg cgaggaacgc gcggctgttg tggaagaggt agttgcggcc
     2341 cacctgatcc gatccgttgc ccagcccgtt gggatgccgg tcgttcgcgc tggcgaggag
     2401 cagcttggct gagttggcgg cacccgctga gagcaccacg attgcgccgg tgaaccgttg
     2461 cggttcaccg ttgacttcgg cgacgaccgc tgtgacagcc ccgccgctcg catccgtctc
     2521 gaggtgccgc acgtgcgcat tgcggaccag ggtgacgttg gcatggtcca acgccggccg
     2581 caccgcgacg acgtccgcat cggacttggc gtggacgaga cacgggaagc catcgcaggt
     2641 ggcgcatcgg atgcagcggc tcagttcggg acgtgactcg tccagcatga tgccggatgg
     2701 gctgtggaag gggtgcaggc ccaccttcgc catgtcgtcg aacagccgct gcatccgagg
     2761 ttcgtgggac accgcggggt agggataggg ttcgctggcc ggcggatcgc tggggtcctc
     2821 tcccctgttg ccgtggacgc agtagagccg ctcggcctgc gtgtagtacg gctccatcac
     2881 gtcgtatccg atgggccagg cgggcgagat cccgtcgtga tgtttgagct ctccgaagtc
     2941 gagttcgcgc agtcggtaca gcgccgcgcc gtagaacttg gtggcgccgc cgacgaagta
     3001 gtggatctgc ggctggaacg gttggcccgt ggcgtcgtac cacgtgtcct tcgacacgta
     3061 gcggttgtcc acgaacaccg cctgcgcatc ccagttctcg atctcgcgag gcagccagtc
     3121 gccgcgctcc aggatcagga tccgatgacc gctcggggcc agtcgatgcg ccaacgtgcc
     3181 gccgcccgcg cccgacccca cgatgatgac gtcgtattcc gctgacatct gccgctcctc
     3241 ccatcggctc catccacggt cggacggcgg cggacggacg gcatcacccc gatggcatga
     3301 aaactgggga cccgcgaggc cgctcttggc gggacatgta cgcgtatggc tggatgattg
     3361 cggtgtctcc atcgcagttg catggtcgct agcgcggatc gcaaaccatt cccggtgctt
     3421 cgtcggcctg cttcagccgt gaagacccag ttcgctgatg cgttcgaccg ccgcggtgcg
     3481 cgacgacaca ccgagcttgc ggtagatgga gctcacctcc gacttgacgg tgtggcgtga
     3541 gatgaagagt cgcgcgccga tctccggcaa cgacaagtgg gtcggcagca agggcaggag
     3601 tcgcaactcg gcagccgtca gggacgacgc accggtgccg gacccgctga tgcgctcgac
     3661 cctcgaccga aggtgctcca cctccccggc gaggttcccg aggtgtggtc gctgctgaag
     3721 gattgccgtg gcctgctcga tcgcagcgcg ggcgccaccc ggatcgacaa cggcgatgta
     3781 ggcgttggcg agctggatca gcgcctggac ggagaccacc ggcagagcgt aggtgagcag
     3841 tcggcgcagt cgcgctgcgc gcctgatgag ccggcgtgcg gacgggatgt ctccagagcg
     3901 cgcacaggat ctcgccgcga tggcgagcac gagcgcgctc gtccagtagc cgtcgaagtg
     3961 gccgtcgtca acgatttcca gtgaccttcg cgcgtacgaa tcggcggcga tccagtcccc
     4021 gcgttcggcg gctacgaggt aacgctcggc gagggtcagc gcggtcagcg ggccgaccgc
     4081 gagctcggtg gccagatcgc acgcgtgggc cagcatcgcg tccgcctggt cgaggtggcc
     4141 ctccaggaga tgggagatcc cttggacgta catcatggtt gctcggtagg gactgtcggg
     4201 actcagcccc gtcagggcag cccccgcgtc acggcgcatc gcggccggcc cgtcccgacc
     4261 gaggttcgca cggagatagg ccatcgtgcc cgcgaccgtg ctcccgtcag gcagggtccc
     4321 ctcggcaggc agccgctctg cgatggcggt ccaacgttcg gcctcactcg cccgaccgag
     4381 gagcgcgaag atgagtgacc catgggctgt gatcgcggcg gagtgctgcg tcggcggccg
     4441 ttcccccagc cattccatcc atccggcgac ggtgtcgacg cgaccgctga cccagaccgg
     4501 ctgcatgacc tgcagcacaa gggcggccac ccgatcggca tcaccggcgg cctgcgcatg
     4561 ctcgatcgcc ttttccggca ggtcattgtt ctcgtaccag gccgaggcgc gggcatgcag
     4621 gctcgggatc tcggccgtcc ggtcggctcg aagctcggcg tacagatgct cgcgcaacag
     4681 atggtgatac cggtaccagc ggcgacgacg gtcgagcggg acgacaagca ggttgcgctt
     4741 ctccagccgc tcgagcagtc gggccgaacc cgtgctgtcg aggaccgcat cacacaacgg
     4801 cccagacata cgatccagga tcgacgtgcg ctggaggaag ccggcctgtg tcttcgtgag
     4861 gctgggaagc agttccgttg cgaggtagtc ccccaccagc cagtcctcac cggtgaagtt
     4921 ctcggcggga gcacctgact tcatggcgag caccgccagg tacagcgcca ccggccagcc
     4981 ctcggtcgtc ccgaccagag cgtcagcctg tgccttcgac aggtcgacgc cggcacttgc
     5041 gagcaaggca cgggcctccg cccccgccat cgcgaggtca ctggcgctca gttcgagaac
     5101 ctccccctgc gttcgcattc gcgccgcgcg aatcgggacc tggtcgcgcg acgacatcgc
     5161 cagctgccaa cccaccggta cccgcagcgc gaactcggcg agcgccgccc gacacgcagg
     5221 tgcggtgatg gtctcgacat ggtcgagtat caccgtcacg ggctcgctca atgagccaac
     5281 cgcggacgcc agcccgggca cggcgtccac accaccgccg tacgctgcca ggatccgtgc
     5341 ggcagccgga ccaaccggct ccaccgcatc cagtcccgtc aggagcgccg tccacaacgt
     5401 gaccgggtcg ttgtccgcga cgtcacacga aacccacacc acgcgcggtg tacgacgcct
     5461 cgcccactgg gccaacagcg tggtcttccc gtatccggcc ggcgcgacaa ccgtgatcac
     5521 cggttcgtcg gcggccacca tgcggtccac cagcgcggtt cgcgtcacca tccatcgacg
     5581 cgccacaggc agctggaact tcggaccaag actgccttca gggcgcgacg agccaccggg
     5641 cgctggcgcc ggttcggcgt cggcccgaag tgtccgaacc tcgtcgctac cgtccccaac
     5701 aggcacggaa cgaacgtagc gcgatgcgga acgctcagga acgcgttccg cccacgggcg
     5761 atgaccaggg cgatgaccag gctgatgacc aggctgatga ccaggctgat gaccagggtg
     5821 atgaccaggc tgatgaccgg tacgggcaga tcgtcgtgac cgtcctggtt cactgagcgg
     5881 gcgactgcgt cgtccggtcg cgtccgcctg gttgcgtgtc ctcgagccgg tagcgactga
     5941 gcagctcctc ttcctcccgc tgcttgggga agcagaagaa gacgagcgtc gcaccgagca
     6001 ccacggccac agcacctgcg ccgtaggccc agtcgtcacc gtcgaggaag gatgtcttcg
     6061 ccgccgccgt gatctggtcg gcgtactgcg ggtagcgctt ggccgtctcc tcggcactgg
     6121 agaaggactt ggtcagctcg ctctgcacgt tcttgctcac ctggtcggca tcgggcgagc
     6181 tggtgatctg cgccgcgaac gcactggcat accccgtggt cagcaaggcc ccgagaatcg
     6241 actgcatgat ggcgccaccg aggtcgcgct gcagatccgc cgtgcccgag gccatacccg
     6301 ctctcgccct cgggaccgaa ccggtgagcg agtgcgacgc cggcgtaccg gccaacccga
     6361 cccctacacc gatgaacgcg tacgcgatgc cgatctgcca gtacgagctt ccttccttcc
     6421 acagcagcag catcgagagg aaggcgagca gcaggaagca gtagccgagc agcaacgtga
     6481 gccgcgaacc gtgggactcg atcaacttcg ccgagcgggg tgcgaccagc accatcatca
     6541 cgatcgcggg caagaacgct gccccggcct ccaacgtcga gtagccgagc acgttctgga
     6601 gatactgctg gctcacgaac gccgcagcca tcagcgagcc gaacacgatg atccctgccg
     6661 tcgcggcaac ccagaagatg cgacgcgacg ccacgcgcag gtcgtagagc gggttcgctg
     6721 cgcgccgttg ccggatgagg aacgccccga cggccgcgac cgcgatcaag gccaggccga
     6781 gggccaggct gagcttgccc ggtacaggcg cgaggttgat ggccagcacg acagcgccga
     6841 cgaggaccac cgagaggatg ccgcccaggt tgtcgaccgg gtcggagtcc tcgttgacgt
     6901 gtgccggcac cagcagccag gccatgacga gtgcgacacc ggccaacggc aacgtgacca
     6961 ggaagacaga tccccaccag aagtgctcca gcatggcgcc ggcgacaagc gggccgagtg
     7021 ccgagatggc gccgccgagg gctgaccaca gtgcaatcga cttcgttcgc ggctggcctg
     7081 cccacaaagc tgtgatgagt gccagcgtcg tcgggtacgc catgcccgcc gacacgcccc
     7141 cgaagatccg ggccccggcg agcacgtact cgttcggtgc gtacgccgcg agcaggcagg
     7201 cggggatcga gagggtcacg ccgagcagca ggagcagctt gcgcccgtac cggtcgccaa
     7261 ccgcgcccag atacagcacc gacccggcga gcccgagcga gtagccgatg gcgatgaggt
     7321 tcagcgacgt ctgcgacgcg tcgaacgcca ggccgatcga aggcagcgca acgttcgcca
     7381 ccgaaaggtt caggttggcg accccggcca ccaggatcaa cgccgtcaag atcagcccgg
     7441 cccgcggtgg agcctcagca gcctcagccg ttgccacctc cgaaggcggc tcgatcgggt
     7501 cctccgccct ggtgtcctcc atgctcagct ccccgtcgca ggtgactcgg ctacgcctcc
     7561 agtgctcgcc agctctcacc aggctcgagc aggctgtcag cttccgacgg tccccaggtt
     7621 ccgcgtgcgt acgtgtgcaa cggtgtcaca tcgcccagca ccgggccgac cacccgccat
     7681 gccgcctcca ccgtgtcctg cctcgcgaac agccactgtt cgtcggccag cgccgcgccg
     7741 atgagccggt cgtacggcct catctcggtg ttcgggcgtt cgatgaatgt cacgtcgttc
     7801 gtctgcggca ccatctccgc tcccggcttc ttgcccgcca ccgtcagtcc gacctgcgtc
     7861 tccggccaga tccggaagcg caggtagttc atgccctcga aggggtcgag gccgaacacg
     7921 ttctgcggtg gcctgcggaa gttgacggtg atctcggttg cggtcaccgg catgcacttg
     7981 ccggcccgga tgaggaaggg gaccccctcc catcgccagg agtccaccgc cagtcgaacg
     8041 gcgacgaacg actccgtggt tgatccggga gccaccccct cgacgtcgag gtagccctcg
     8101 tactgtccgc gcacgacgtc gtcaggagtc agcgggcgaa tcgccttgac caggccggcg
     8161 cggacgtctc ggacgccgcc cagcccgccc tgcggcgggt cgatcgtcac tgcggcgagc
     8221 acctggagca tgtggttctg cagcacgtct cggatcgcac ctgttcggtc gtagaaagcg
     8281 ccccggtcgg ccacgtcgaa cgcctcggcc atcgtgatct ggatgttctc gacgtaggtc
     8341 cggttcatca cggcctcgaa gagcgtgttg gcgaacctgg tgaacaggac gttctcgacc
     8401 gggtcgaggc cgagccagtg gtcaacccgg aagatcgcat cctcggagaa gaccttgtgc
     8461 atggtctcgt tgagcttccg tgcactctcc aagtcggtgc cgaacggttt ctccaccatg
     8521 acgcgagcac catcggtcaa gccggccttg ccgagtccgt cggcgatcgt gccgaacaat
     8581 ggcgggggca cctcgaggta gaagaggacg cgggcgccgt ccggcagctt gtcggcaagg
     8641 gtctggtacg tcgccgcgtc cgcaaggtcg ccgtcgacgt actcgagaag accgagcatg
     8701 cgcttgccgg acccggaatc gggatcgagg tcgttgcgcc gcaacgagtc ggcggcgtag
     8761 ttgcggaagt cctcgagtcc ccagccactc ttggcgacgc cgatcaccgg tacgtcgagg
     8821 agtccacgtt cgacgagtcc gacgagagca ggaaacgtct cgagtttggc caggtcaccc
     8881 gtggccccga aaatgacgag agcgtcgact ggctgtgtgg tggtcatcta ctgcctccca
     8941 cggttgagtg ctcggtccag cgtgatcgcg gcgtcgatca gcgccaggtg ggtgaaggcc
     9001 tgcgggaagt tgccgagctg ctcgccggtc aaggcgatct cctcggagaa gagcccgagg
     9061 tggttcgcgt acgtcagcat cttgtcgaag gtgagcctcg cctcttcgag acgtcccgct
     9121 cgggccagag cgtccacgta gttgaacgtg cacagagaga aggtcccctc gtcgccggtg
     9181 agcccgtccg gtgacgccgc cgggtcgtat cggtagacga ggctgtcggt gaccagctcc
     9241 tgctccatcg cgttcagcgt cgacgcccac atcgggtccg tcggtgcgat gaacccgacg
     9301 gtcgccatcc gcagcagtga cgagtcgagc acgtccgagc cgtagtgctg gatgaacgcc
     9361 tctcgcttct cgctccagcc cttgtccatc acctgtgcgt agatcgcgtc gcgggcctga
     9421 acccagcggt tgatgtccgc aggccttccc atgctgcccg ccatccggat cgctcggtcg
     9481 aacgcgaccc agctcatcac gcggccgtac gtgaagtcct gctggccgcc ccggctctcc
     9541 cagatgccct ccccaggctg gtcccagttg tcgcagagcc agtcgatcat ctccgtgatg
     9601 gcgagccacc catggtggcc gacgtcgatg ccgtgctcgc tcgcgaagaa cacgctgtcc
     9661 atcgcctcgc cgaagatgtc catctggacc tgggtggcgg cgccgttgcc gatgcggacc
     9721 ggtcgcgagt gctgataccc gtcccagtcg tccagcgact cctcgacgag gtccgacgac
     9781 ccgtccacgc ggtacatgat gtcgagcggc cgcccggagg cgctgtcccc acggatgcgg
     9841 tctcgcagcc atgtcaggaa tcccatcgcc tcgtcggtga agccgagacc gagcagcgcg
     9901 tagaccgaga aggacgcgtc ccggacccag gtgtagcggt agtcccagtt ccgctcaccg
     9961 ccgagctgct ccggaagccc cgcggtcggg gccgcgacga gtgccccgct gggcgcgtag
    10021 gtcatgagct tgagcgcgac ggctgaccgg tgtaccgcct ctcgccaccg cccggtgtac
    10081 gtcgactgat cgagccagtc acgccagtac gccacggtct cgtcgaacat ctgctggaac
    10141 tcggcgaccc tgatctcgcg cggcggacct tcggcagcgc tctccagcac gatcccccga
    10201 acctgccccg cctgcagcgt gagcgaaatc gcgatgtcgc cgtctgcgtc cagcgtcgga
    10261 cggatgaggt gctcgtcctc cggctctcgg acgacatgca tggtcagcga cagctcaccg
    10321 tcgtcgagca cgacgccggg ctcgacgacg ctggtcttgt gcggccggcg accgtggtcg
    10381 aagcgtggct tgagcaggcc cttgaactcc atcgagccac gtacgcaccg caccctgcgg
    10441 acgagacgat ggttgtcagt ggccggttga cccgggaggg ggatcatgaa gtcggtgatc
    10501 tctccgacgc cgtcagcggt gaggaaacgc gtcagcagga tggccgtgtc cggaatgtac
    10561 agctgcttgg tcgtatacgt ctcggccgcc ggcgtgatcg tgagctcacc acccttctca
    10621 cgatccagta gcgacccgaa gacgctcggc gagtcgaacc gcggaaggca caaccagtcg
    10681 atcgtgccgt ctgtggtgac cagcgccgac gtctgcaggt ctccgatcag cccgtgctct
    10741 gcaatgggcg ggtagtgctc cataaccggc tcctcctgcg catgcccgtt gcccggccag
    10801 cggccgggca cgactccgga cgtctccagc cttggctccc acgggcgggc acagcatcac
    10861 ccgaagcaga tgagttctca ggtctcctcg gctccgaccg gctcctccag cccctcctcg
    10921 ggcagcagac tccgctggtc gagcacgtac aggaggacga aggcaggcac gatgagcacc
    10981 gccgccaggg cggccgcgac caacactgcc gtgatcgtgc cgtccggcgc ggccgcgtcg
    11041 gagatcgtca atgtggtggg aagcaggtag tcccactgcg ccacacccca ccccaccacc
    11101 agtgcgacca ccgccagcac cgctgcgggc cgggcgacaa catgcgaatc cctgcgaagc
    11161 aggaccaaag ccgcgcaacc ggcgatgacg gagaggatgg cgagggggag cccacgcgac
    11221 atcaggccgt cgaagacgta ggtcgcctcg tcgtgcagca cgaagagccc ggccgaagca
    11281 acgacgcccg cgacgaccgc cgctgccacc gctctgcgcc ggaagtacgc caccatgacc
    11341 tcgtcggaga gccgacgcgc gtcccagacc aggaagaccg cagcgaggta cgccgccagc
    11401 gagaccgcca acacgcccgc aaggacggac gtggcgttca cccagctgct ccaggggtcg
    11461 ccggcctccc cgccggccgg gatccggccc gccgccacgg ctccggcgac ggccccgagg
    11521 cagtacggca ccagaaccga cgacacggcg aagacggcgc cgaagttccg ccgctcgcgg
    11581 gtgctgaaca ccgccttgcg gaaggcgaag ctcgcccccc ggagcacgat ccccaaggcg
    11641 gccaacgtga gcggcacgaa catcgtcagc gtgatcgact cgaacgcctc gggaaagcag
    11701 gtccacaaca cgacgaaggt gaagacgagc cagacgtggt tcgcctccca caccggtgcc
    11761 acggcgtggt cgatcacctc acggggacgg tcaccacggt gtgcccctcc ggcgacgagg
    11821 tcccagaacc cggcgccgaa gtccgcacct ccgaacaccg catagatcgt caccgcggcg
    11881 aacagcagga cggcggccac gtcactcatc cgaccggtac ctccggctcg accgtcacgt
    11941 cggacgggcc gtagggcgcg tcacggtcgt cgaacccgcg ttgctgccgg aaccggcggc
    12001 tcatggcacg gaggacgagg atgatcgtcg tgccgagtgc ggcgtacagg atgatcacgg
    12061 cgacgaacgt gatccagacg ccggtgttcg cggtggcggc gtcctcgacc ttcatcttct
    12121 cgtagacgat ccacggctga cgtccgacct cactgaccgt ccagccggcc tcgagcgtga
    12181 cgactgacag cgccccagcg actgcgcaac agcggaggaa ccaccggctc ttcgggatgt
    12241 ctcgtcggaa gatccacgag gcccagtacc acagcgtcaa caggaacaac agggtcccga
    12301 cacccaccat cacgtcccag gcgaggtgca cgacgttgac ctcgtggatg gatgggcgct
    12361 cactcgacgg cacggtgtcg aggccctgga cgaccgtcga cttcccctcg ctgggatccg
    12421 acaaccagga tgcgagcccg gggatgggaa tcccgccggt cacttcccct tcggagttga
    12481 gatggccgag cagtgtctcg ggcacgtcgc tgctcgtctc ggggaccatt tcgatcgccg
    12541 cgaacttgac cggctggttg tcgtacaccc atcgggccag ggtgtcgccc acacccatct
    12601 gcaccggcgt tgcgatcgcc gccacggtga agccgatgat gaagcccagg cggtggtaac
    12661 ggtcacggcg accacgcaac atgcccacgg cgtacaccga cgcgatcaga aatccaccga
    12721 cgaggtatgc cgcgatgacc atgtgcgccg ccatcagcgg catggcgtcg ttgaggatca
    12781 cttggacggg gtcgacgtcg acgatgttgc ccgacgcgtc gagtgtcacg ccctgcggcg
    12841 agttcatcca agcattggct gcgacgatcg agatgctgcc gaacactccg gcaaccggga
    12901 tcggcacgcc ggtccagaag tgcgcccacg gtctcaaccg tcgccagccg aagatgtaga
    12961 tggagatgaa gaccgcttcg gcgaagaaga agatgccttc gaacgcgaat gggataccga
    13021 atgcttgccc ccactgcccc atgaacttgg gccagagcag cccgaactcg aacgtcagca
    13081 ccgttccggt caccgcgccc acggcgaacg tcaccgccat gtacttcgac caacgctgcg
    13141 cgagcagcag cgcgtcggcg tcgtcgtgct tga
//
LOCUS       DATBFZ010000554         6373 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_4578447, whole genome shotgun sequence.
ACCESSION   DATBFZ010000554 DATBFZ010000000
VERSION     DATBFZ010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 6373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6373
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4578447"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1616)
                     /locus_tag="VH419_16920"
     CDS             complement(<1..1616)
                     /locus_tag="VH419_16920"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HEY7045356.1"
                     /translation="MRRRWMLVLAALVATAAVASACGRDASAMPEAPRYVDETGAAGI
                     VHRYDGDADFFVGGGVAAFDCNDDGRDDLYFAGGSQPAALYRNESPVGGALRFRPQPS
                     PVTDLTSVTGAYPLDIDGDGHTDLAVLRRGGNVILRGLGDCRFEPANDRFGIDGGHAW
                     TVGFSATWEGTNALPTLAFGNYLVPGTYGCDTSELLRPASVAGRYASPIALQPGYCAL
                     SALFSDWDRSGERDLRLANDRNYYRDGSEQLWQVAPGAAPRLYTEKDGWRPLQIWGMG
                     IASQDLTGDGRPEVFITSQADNKLQTLARGDARPTYEDIALERGVTAQRPYSGGDVLP
                     STAWHPEFADVNNDGFVDLFITKGNVDAQVDYATRDPNDLLLGQGDGTFVEGAKQAGI
                     VGFERSRGAALVDLNLDGMLDVVVVNRRANVKVWRNVGRGDAERPRPVGHWIALQLQQ
                     PGPNRGAIGAWVEVKVGDRRIDREVTVGGGHAGGQLGWIHVGLGDHDATQVRVQWPDG
                     ETGPWMDARADEFVKIERGATEVERWDPEV"
     gene            1680..3272
                     /locus_tag="VH419_16925"
     CDS             1680..3272
                     /locus_tag="VH419_16925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7045357.1"
                     /translation="MTSRTLTLGHTTYPVILPNARDARVHVAAVIITIHVLGQVGLRF
                     DVSVPQILAAIVTCAVLEIALTFRARKAFVWPASAMLTGSGVALILRVPGTPPGDHWT
                     TYKWYVFAGVAALSLVTKYAIRYRGSHVFNPSNLGLVVAFLLLGSTRVEPLDFWWAPW
                     TRWMILAYAVILVGGLLITARLQLLAAAVMFWGALAAGIGVLAASGHCMTARWAFAPV
                     CGFDYWRVIVTSPEVMIFLFFMLTDPKTVPAGRVGRVVFALLVAVTSTLLMATQSQEF
                     GTKVALLGGLVLVCAARPLVDRLVPEPRSTADDLRRFASRLALPSGPGGHVVGGALRL
                     AVIAAVCLGLTAGIVAAGSPARGVVVPAAAEVLNRVPHDVDPATLPKISVDQDVLDWN
                     HEISGPGAQEIVLTLVENLELEDQALRRADESILTAVDHGDRLEQMRDRLDAAVSSGT
                     TTVEHYRIDDVHVTLLVPFGRQDGLSLGLESRGTETRETYDSAGHLRSRTSSPFATTF
                     VVRRATGARWLNVAQLRPGADR"
     gene            3385..4473
                     /locus_tag="VH419_16930"
     CDS             3385..4473
                     /locus_tag="VH419_16930"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEY7045358.1"
                     /translation="MRARSLLAVTMGFGLLLLPACGSSDNNSSSGTTAGAGGTSGGCK
                     VGVSWNNYQEERWAKFDEPAIKKAVEDGGGTYISNDAKSSAETQASNVENLMSQGANV
                     LIILAQDGTAIKPSVTEALSNKVPVIAYDRLIEDPGALYITFDNVLVGQLQAESIFKA
                     VPKGNYIIIKGNKADANADFLRSGMDKVIKSAVDSGDIKIVGETYTDNWDPSKAQTET
                     EQFLTAANNDVQAVLSENDGMAGGVVSALQAQGLAGKVPVSGQDGDQAALNRIALGTQ
                     TVDVWKDARLLGKTAGESAIQLCKDKDVTKVSGTSSFDSPGGNQLTSILLKPQPITKD
                     NLDVVIDAGWTTKDNVCKGVTAGSVPVC"
     gene            4508..5899
                     /locus_tag="VH419_16935"
     CDS             4508..5899
                     /locus_tag="VH419_16935"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7045359.1"
                     /translation="MTETEAPSAFGTSPTLGPSPQTTPTPARERGWRSLLASTELDTR
                     LLGMVVALAAIWITFNILSDGDFLTSRNLWNLSVQSTSIAIMATGMVLIIVSRNIDLS
                     VGSLLGFLGYTMALVQTDGVIAFFGLKVTIGGLADKPYVWVVALLVGVVFGALIGSLM
                     GYIVAYGGVPSFIVTLGGFLVWRGMIFRTGGKQGQTLAPLDHIFQLIGGGAKGSLGEW
                     RSWGFALLGCAGVVLSIYLARRRRSRYDLAVRPILVDVGLGIVGCAAVIAGVGLIANR
                     YDSPITGKPTGVAYPVVILIGVTLLMTYLAKRRRFGRYVYAYGGNPEAAELSGINTRR
                     TVMLTFSLMGVLVAVSAAIQTARLNAAVTNLGVQNELDVIAAAVIGGTSFSGGIGTIP
                     GAVLGAIVMQSLRSGMVLLRIDSPSQDIVVGVVLVAAVAVDSALRAGRRRAALGALVI
                     GLALAAIVIQTGR"
     gene            5921..>6373
                     /locus_tag="VH419_16940"
     CDS             5921..>6373
                     /locus_tag="VH419_16940"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY7045360.1"
                     /translation="MTDTQPAPIPSEAGLTPLVEMRDIRVAFGGLHAVDGVSVDLYPA
                     EVVALVGGNGAGKSTLIHTLSGAHPADSGQILVNGEPVTIHTPRDAQKYGIETIYQKL
                     ALAENIDAAGNVFLGRELRTRTGALDDSAMASATQKVVRRLNPRFTNLK"
BASE COUNT          954 a         2261 c         2142 g         1016 t
ORIGIN      
        1 tgaacctccg gatcccagcg ctcgacctcg gtcgcacccc gctcgatctt cacgaactcg
       61 tcggcccgcg cgtccatcca cggccccgtc tctccgtcgg gccattggac gcggacctgg
      121 gtggcgtcgt gatcgccgag cccgacgtgg atccagccga gctggccacc ggcgtggccg
      181 ccgccgacgg tgacttcgcg gtcgatgcgc cgatcgccga ccttcacctc cacccacgca
      241 ccgatggcac cccggttcgg ccccggctgc tggagctgca gcgcgatcca gtgaccgacg
      301 ggacggggcc gctcggcgtc gccccggccg acgttgcgcc acaccttgac gttcgcgcgc
      361 cggttgacca cgacgacgtc gagcatgccg tcgaggttca ggtcgacgag cgcggccccc
      421 cgactccgct cgaagccgac gatgcccgct tgcttcgcgc cctcgacgaa cgtgccgtcg
      481 ccctggccca gcaggaggtc gttcggatcg cgagtggcgt agtcgacctg ggcgtcgacg
      541 tttcccttgg tgatgaacag gtcgacgaag ccgtcgttgt tgacgtccgc gaactcgggg
      601 tgccatgcgg tcgacggcag cacgtcgcca cccgagtacg gccgctgggc cgtgacacca
      661 cgctcgaggg cgatgtcctc gtacgtcggt cgtgcgtcgc cgcgggccag cgtctgcagc
      721 ttgttgtccg cctggctggt gatgaacacc tcgggccggc cgtctccggt gaggtcctgg
      781 ctggcgatgc ccatccccca gatctgcagt ggccgccaac cgtccttctc ggtgtagagg
      841 cgcggcgctg ctcccggggc gacctgccac agctgctcgg atccgtcgcg gtagtagttg
      901 cggtcgttgg ccaagcggag gtcgcgctca cctgaccggt cccagtcgct gaacagcgcg
      961 gacagcgcgc agtagccggg ctggagcgcg atgggcgatg cgtagcgccc tgccacgctc
     1021 gcgggccgca ggagctcgct cgtgtcgcag ccataggtgc cgggcaccag gtagttgccg
     1081 aacgccagcg tgggcaacgc gttcgtgccc tcccacgtcg cgctgaaccc gacggtccac
     1141 gcgtgaccgc cgtcgatccc gaagcggtcg ttggcaggtt cgaaccggca gtcgccgagc
     1201 ccgcggagga tgacgttgcc accccggcgg aggacggcca ggtccgtgtg gccgtcgccg
     1261 tcgatgtcga ggggatacgc accggtgacc gaagtgaggt cggtgacagg cgacggttgg
     1321 ggacggaagc ggagcgcgcc acccaccggg ctctcgttgc ggtacagggc cgcgggctgc
     1381 gaaccgccgg cgaagtagag gtcgtcgcgc ccgtcgtcgt tgcagtcgaa ggcggcgacg
     1441 ccgccaccca cgaagaagtc ggcgtcgccg tcgtagcggt ggacgatgcc cgcggctccg
     1501 gtctcgtcga cgtaccgcgg cgcctccggc atcgctgacg cgtcacgacc gcacgcggat
     1561 gccacggcgg ccgtcgcgac cagcgcggcg agcacgagca tccaccgacg tcgcacccac
     1621 ggattgaacc acgacctaga gtccccggga cgaccagtca tgcgttgggt ggagagggtg
     1681 tgacgagccg gacgctgacg ctcggccaca ccacctaccc cgtgatcctg ccgaacgcgc
     1741 gtgatgcgcg tgtgcacgtg gcggccgtca tcatcacgat ccacgtcctc ggacaggtcg
     1801 ggctgcggtt cgacgtcagc gtgccgcaga tcctggcggc catcgtcacc tgcgcggtcc
     1861 tcgagatcgc actgaccttc cgcgcccgga aggcgttcgt gtggcccgcc agcgccatgc
     1921 tcaccggcag cggggtggcg ctcatcctcc gggtgcccgg aaccccgccc ggcgaccact
     1981 ggaccaccta caagtggtac gtgttcgccg gcgtcgcggc gctgtcgctc gtgacgaagt
     2041 acgccatccg gtaccgcggg tcgcacgtgt tcaacccgtc caacctcggc ctggtcgtcg
     2101 ccttcctcct gctcggcagc acgcgggtcg agccgctcga cttctggtgg gccccatgga
     2161 cgcgctggat gatcctggcc tacgccgtca tcctcgtcgg cgggttgctg atcaccgctc
     2221 gcctgcagct gctcgccgcc gcggtcatgt tctggggcgc gcttgcggcg ggcatcgggg
     2281 tgctggccgc ctccgggcac tgcatgaccg cccggtgggc gttcgcgccc gtgtgcgggt
     2341 tcgactactg gcgggtgatc gtcacgtcgc ccgaggtgat gatcttcctc ttcttcatgc
     2401 tcacggatcc gaagacggtt cccgccggcc gggtcggccg cgtcgtgttc gcgctcctcg
     2461 tcgccgtcac cagcacgctg ctgatggcga cccagagcca ggagttcgga accaaggtcg
     2521 ccctcctcgg tgggctggtg ctcgtctgcg cggctcgccc gctcgtggat cggctggtgc
     2581 ccgagccgcg atcgacggcc gatgacctcc gccggttcgc ctcgaggctg gcgctcccga
     2641 gcgggcccgg cggacacgtg gtcggtggtg cgctccggct ggcagtgatc gcggcggtgt
     2701 gcctcggcct caccgccggc atcgtcgccg cggggtcacc cgcccgcggc gtcgtcgtac
     2761 ctgcggcggc cgaggtcctg aaccgggtgc cccacgacgt ggacccagcg accctgccga
     2821 agatctccgt ggaccaggac gtcctcgact ggaaccacga gatctccggt cccggcgcgc
     2881 aggagatcgt cctgaccctg gtcgagaacc tcgagctcga ggaccaggcg ctgcggcgcg
     2941 ccgacgagtc gatcttgacg gccgtggacc acggggaccg gctggagcag atgcgagacc
     3001 gccttgacgc ggcagtttcg agcggaacga ccaccgtcga gcactaccgc atcgacgacg
     3061 tgcacgtcac gttgctcgtc ccgttcggtc ggcaggacgg cttgagcttg gggctggaat
     3121 cccggggcac ggagacccgc gagacctacg acagcgcggg gcatctccgc tcccggacct
     3181 cgtccccgtt cgcgacgacg ttcgtcgtcc gccgggcgac gggagcacgt tggctcaacg
     3241 tcgcgcagct gcgacccgga gccgatcgct gaacggcgcg gcggccggac aggatcgacg
     3301 ctttgtcgcc gaccgtgtgc taagagtgtg gccttctcga ggcgaccgac tacctcgcaa
     3361 catgcagaga gggagggctc tccgatgcgt gcacgtagct tgcttgccgt cacgatgggt
     3421 ttcgggctcc tgctgctgcc cgcgtgcggc tccagcgaca acaacagctc gtcgggcacc
     3481 acggcgggcg caggtggcac ctccggaggg tgcaaggtcg gcgtctcctg gaacaactac
     3541 caggaagagc gctgggccaa gttcgacgag cccgcgatca agaaggccgt cgaggacggc
     3601 ggcggcacct acatctcgaa cgacgccaag tcgtcggccg agacccaggc cagcaacgtc
     3661 gagaacctga tgtcacaggg cgccaacgtg ctgatcatcc tcgcccagga cggcaccgcc
     3721 atcaagccgt cggtcaccga ggcgctctcg aacaaggtgc cggtcatcgc ctacgaccgc
     3781 ctcatcgagg accccggcgc tctctacatc accttcgaca acgtcctggt gggtcagctc
     3841 caggccgagt cgatcttcaa ggccgtgccg aagggcaact acatcatcat caagggcaac
     3901 aaggccgacg ccaacgccga cttcctacgc tccggcatgg acaaggtcat caagagcgcg
     3961 gttgattccg gcgacatcaa gatcgtcggc gagacctaca ccgacaactg ggatccgtcc
     4021 aaggcccaga ccgagaccga gcagttcctc accgcggcca acaacgacgt gcaggcggtt
     4081 ctgtcggaga acgacgggat ggccggcggc gtcgtgtccg cgctccaggc gcagggtctc
     4141 gccggcaagg tgccggtgtc cggtcaggac ggcgaccagg cggccctcaa ccggatcgcg
     4201 ctcggcacgc agaccgtcga cgtctggaag gacgctcgcc tgctgggcaa gaccgccggc
     4261 gagtcggcca tccagctgtg caaggacaag gacgtcacca aggtgagcgg aaccagttcg
     4321 ttcgacagcc ccggcggcaa ccagctcacc tcgatcctgc tcaagccgca gccgatcacc
     4381 aaggacaacc tcgacgtggt catcgacgcc ggctggacga cgaaggacaa cgtgtgcaag
     4441 ggcgtgacgg ccggttcagt acctgtctgc tgatcggttc tgctgatcgg ttccacgagc
     4501 gtggacgttg accgagactg aggctccatc tgcattcggg accagcccga ccctcggccc
     4561 gagtccccag accaccccga ccccagcgcg cgagcgcggc tggcgctcgt tgctggcctc
     4621 gaccgagctg gacacccgcc tgctcggcat ggtggtggcc ctcgcggcca tctggatcac
     4681 gttcaacatc ctgtcggacg gcgacttcct gacgtcccgc aacctctgga acctgtccgt
     4741 ccagagcacc tcgatcgcga tcatggcgac ggggatggtc ctgatcatcg tgtcgcgcaa
     4801 catcgacctg tcggtcggtt cgctgctcgg cttcctcggc tacacgatgg ccctggtgca
     4861 gaccgacggc gtcatcgcct tcttcggtct gaaggtcacg atcggcggat tggccgacaa
     4921 gccgtacgtg tgggtcgtcg cgctcctcgt cggcgtggtg ttcggcgccc tcatcggttc
     4981 gctgatgggc tacatcgtgg cgtacggagg ggtgccctcg ttcatcgtca cgctgggcgg
     5041 cttcctcgtc tggcggggca tgatcttccg caccggcggc aagcagggac agaccctcgc
     5101 gccgctcgac cacatcttcc agctgatcgg cgggggagcg aagggctcgc tcggcgagtg
     5161 gcggagctgg gggttcgcac tgctgggctg tgccggcgtg gtgctcagca tctacctcgc
     5221 gcgccgccgc cggagtcggt acgacctcgc cgtgcgcccc atcctcgtgg acgtcggact
     5281 gggcatcgtg gggtgcgcgg cggtgatcgc cggggtcgga ctgatcgcca accgctacga
     5341 ctcgccgatc acgggcaagc ccacgggcgt ggcgtacccg gtggtcatcc tcatcggcgt
     5401 gaccctcctg atgacctacc tcgccaagcg ccgtcgcttc ggccgctatg tgtacgccta
     5461 cggcggcaac ccggaagccg cggagctgag cggcatcaac acccgccgga cggtcatgct
     5521 cacgttctcc ctcatgggcg tgctcgtcgc cgtgagcgcc gcgatccaga cggcgcggct
     5581 caacgcggcg gtgacgaacc tcggtgtcca gaacgagctc gacgtcatcg cggccgccgt
     5641 catcggcggc acgtcgttct ccggcggcat cgggacgatc cccggcgcgg tcctcggtgc
     5701 catcgtcatg cagtcgctgc gctccggcat ggtgctcttg aggatcgact cgccgtcgca
     5761 ggacatcgtg gtgggtgtcg tcctcgtcgc cgcagtcgcg gtcgactccg cactcagagc
     5821 cgggcgccgg cgagcggcgc tgggcgcgct cgtcatcggc ttggcgctgg ctgccatcgt
     5881 catccagacc ggccgctgag caagcgagag cgacggcacg atgaccgaca cccagcccgc
     5941 cccgatcccg tccgaagcgg ggctcacgcc gctcgtcgag atgcgcgaca tccgcgtcgc
     6001 cttcggcggc ctccacgccg tcgatggcgt cagcgtcgat ctctaccctg ctgaggtcgt
     6061 cgccctcgtc ggcggcaacg gcgcggggaa gtcgaccttg atccacacgc tgtccggcgc
     6121 gcaccccgcg gactccggac agatcctcgt caacggcgaa ccggtgacca tccacacccc
     6181 gcgtgacgcc cagaagtacg gcatcgagac gatctaccag aagctggcgc tcgccgagaa
     6241 catcgacgcc gcgggcaacg tcttcctcgg ccgggagctc cggacccgca ccggcgccct
     6301 cgacgactcc gccatggcgt cggcgaccca gaaggtcgtc cgccggctca acccgcggtt
     6361 cacgaacctc aag
//
LOCUS       DATBFZ010000555         2506 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_5559791, whole genome shotgun sequence.
ACCESSION   DATBFZ010000555 DATBFZ010000000
VERSION     DATBFZ010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5559791"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2288
                     /locus_tag="VH419_16945"
     CDS             <1..2288
                     /locus_tag="VH419_16945"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020575726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HEY7045361.1"
                     /translation="KVVFTREVPSTGSATEVLAYPDTCVGTDSHTTMVNGLGVVGWGV
                     GGIEAEAAMLGQPVSMLIPRVVGFKLSGELPEGATATDLVLTITEMLREHGVVGKFVE
                     FYGSGVGSVPLANRATIGNMSPEYGSTIAIFPVDEETLNYLRFTGRSDEQVALVEAYA
                     KEQGLWLDPDKEPRFSEYLELDLATVVPSIAGPKRPQDRISLSDAKEGFRGVLDEYVK
                     SEDTEDEKGYDEAAAESFPASDPPSYHGDNGSAPPHGKPSAVTDPQGRPSRPVLVKLE
                     DGSEFEIDHGAVAIAAITSCTNTSNPSVMIGAALLAKNAVDKGLTRKPWVKTTLAPGS
                     KVVTDYYERAGLTPYLEKLGFNLVGYGCTTCIGNSGPLIPEVSQAVNDADLAVVSVLS
                     GNRNFEGRINPDIKMNYLASPPLVVAYALAGSMDVDLLNDPLGQDTDGNDVFLHDIWP
                     SPKEVEEIVGTAITAEMFAKDYSDVFAGDERWQNLPTPEGDTFAWDDESTYVRKPPYF
                     DDLPAEPTPVEDISGARVLAKLGDSVTTDHISPAGAIKKDSPAGKYLAEHGVEPRDFN
                     SYGSRRGNHEVMIRGTFANIRLRNQLAPGTEGGFTKSFIDPSTDSGQAEGEVTTIYDA
                     SVAYAEAGTPLVILAGKEYGSGSSRDWAAKGTALLGVKAVIAESYERIHRSNLIGMGV
                     LPLQYPEGENAESLGLTGEETFEITGVTELNEGSTPRTVKVKAGDTELDAVVRIDTPG
                     EADYFRHGGIMQYVLRSLLNA"
     gene            2313..>2506
                     /locus_tag="VH419_16950"
     CDS             2313..>2506
                     /locus_tag="VH419_16950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012921366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEY7045362.1"
                     /translation="MGVDPWVWAVLALAVFTGATVQGLVGLGIGLVAAPVTTLLEPSL
                     MPALLLWLAFVMPVVTLARE"
BASE COUNT          428 a          846 c          849 g          383 t
ORIGIN      
        1 cgaaggtggt cttcacccgc gaggtccctt cgacaggctc tgccaccgaa gtcctcgcct
       61 acccggacac ctgcgtaggc accgactcgc acacgacgat ggtcaacggg ctcggcgtcg
      121 tcggctgggg cgtcggcggg atcgaggcgg aggccgccat gctcggccag ccggtgtcga
      181 tgctgatccc acgggtcgtc ggcttcaagc tttcggggga gctgcccgag ggcgcgaccg
      241 cgaccgacct cgtgctgacg atcaccgaga tgctgcgcga gcacggcgtg gtcgggaagt
      301 tcgtcgagtt ctacggctcg ggcgtcggct cggtcccgct cgcgaaccgg gccacgatcg
      361 gcaacatgtc gccggagtac ggctcgacga tcgcgatctt cccggtcgac gaggagaccc
      421 tgaactacct ccgcttcacg gggcgtagcg acgagcaggt cgcgctggtc gaggcctatg
      481 ccaaggagca gggactctgg ctcgaccccg acaaggagcc tcgcttctcg gagtacctcg
      541 agctcgacct ggccacggtg gtgccgtcga tcgccggtcc gaagcgcccg caggaccgga
      601 tctcgctctc cgacgcgaag gagggcttcc gcggggtgct cgacgagtac gtgaagtccg
      661 aggacaccga ggacgagaag ggctacgacg aagcggctgc cgagtcgttc cccgccagtg
      721 acccgccgtc gtaccacggg gacaacggct cagcgccccc gcatggcaag ccgtccgccg
      781 tgaccgaccc gcaagggcgt ccgagccggc cggtgctggt gaagctcgag gacggtagcg
      841 agttcgagat cgaccacggc gccgtggcga tcgccgcgat cacgtcgtgc accaacacct
      901 ccaacccctc ggtgatgatc ggcgccgccc tgctcgcgaa gaacgctgtc gacaagggcc
      961 tgacccgcaa gccgtgggtc aagaccacgt tggcaccggg ctcgaaggtc gtcaccgact
     1021 actacgagcg cgccggcctg acgccgtacc tcgagaagct cggcttcaac ctcgtcggct
     1081 acggctgcac cacgtgcatc ggcaactcag gtccgctgat ccccgaggtc agccaggccg
     1141 tcaacgacgc cgatctcgcc gtggtctcgg tgctctccgg caaccgcaac ttcgaaggcc
     1201 ggatcaaccc cgacatcaag atgaactacc tggcgtcgcc gccgctcgtg gtcgcctatg
     1261 cgctcgccgg ctccatggac gtcgacctgc tcaacgaccc gctcggccag gacaccgacg
     1321 gcaacgacgt cttcctgcac gacatctggc cgtctccgaa ggaggtcgag gagatcgtcg
     1381 gcaccgcgat cacggcggag atgttcgcga aggactactc cgacgtcttc gccggtgacg
     1441 agcgctggca gaacctcccg accccggagg gtgacacgtt tgcatgggac gacgagtcga
     1501 cgtacgtccg gaaaccgccg tacttcgacg acctgccggc cgagccgaca ccggtggagg
     1561 acatcagcgg cgctcgcgtg ctcgccaagc tcggcgactc ggtgacgacc gaccacatct
     1621 cgccggcggg tgccatcaag aaggactcac cggctgggaa gtacctcgcc gagcacggtg
     1681 tcgagccgcg cgacttcaac tcctacggct ctcgacgcgg caaccacgag gtgatgatcc
     1741 gcgggacgtt cgcgaacatc cggctgcgca accagctggc acccggcacc gagggtgggt
     1801 tcacgaagag cttcatcgac ccttcgacgg attcaggaca agcggaaggg gaggtgacca
     1861 cgatctacga cgcgtcggtg gcgtacgccg aggcggggac cccgttggtg atcctggccg
     1921 gcaaggagta cggctcggga tcgtcgcgtg actgggccgc caagggcacg gcgttgctcg
     1981 gtgtcaaggc cgtgatcgcg gagtcctacg agcggatcca ccggtccaac ctgatcggca
     2041 tgggtgtgct gcctctgcag taccccgaag gggagaacgc cgagtcgctc ggcctcaccg
     2101 gcgaggagac gttcgagatc accggcgtga ccgagctgaa cgagggctcc accccacgga
     2161 cggtcaaggt caaggccgga gacaccgagc tcgacgcggt cgtacgcatc gacacgcctg
     2221 gggaggcgga ctacttccgc cacggcggga tcatgcagta cgtgctgcgc agtctgctga
     2281 acgcctgacg tcggccacga ggttctcgcc cggtgggagt cgatccgtgg gtctgggctg
     2341 tcctcgccct ggcggtgttc accggcgcca ccgtgcaggg cctggtcggt ctggggatcg
     2401 ggctcgtcgc ggcaccggtg acgacgctgc tcgagccgag cctgatgccg gcgctgttgc
     2461 tctggctggc cttcgtcatg ccggtcgtca cgttggcgcg tgagca
//
LOCUS       DATBFZ010000556         2865 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_6469817, whole genome shotgun sequence.
ACCESSION   DATBFZ010000556 DATBFZ010000000
VERSION     DATBFZ010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2865
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6469817"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2167
                     /locus_tag="VH419_16955"
     CDS             <1..2167
                     /locus_tag="VH419_16955"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HEY7045363.1"
                     /translation="MWTIAMPPADTGGGNQPPVAAFSSDCTDLDCTFTDHSTDSDGTV
                     TAHAWHFDDGTTSNQQSPAHAFPSAGSYDVTLTVTDDAGDSDSVTHTVNVDDGSQSSA
                     ISFVGIGHSEPQSQKFKQVTIPAGAQVGDVALMEFTRASTVAWTGPSQVTGWTQVDSF
                     TASGLSSTVWTKVLTASDLGGTVRFDTANFAKALLTVSVYSGVSAGVPVSATHASDTS
                     RTAHVSPSIVASQGDWVVSSWVDRSSTTTGWAAPGTMTSRDVVLSDVSGRYSGVVADS
                     GGPVPAGTFGGKTATSTPATVALMWTVKLAAGTSAAADVVDEINCNFGDDDASLWIHY
                     RMLDGQPTENSVQYGTTAAYGMTATAVAPGILPVDRAGVFREARLTGLSPGQLVHYRI
                     GPNGLDHTCPMPPIGDFRWDDVGDTGTTLCTPWVATTHALLAADEPDFVTHGGDLSYA
                     NVCGTPAVHQYYVDQQVWSTKAAFQPTWGNHEYGQPEDEAPPGTPRDRLANYKSRSTI
                     THAQTLAIDGPNRLSPPGCPAVAGSSTNSCRGEDWGWFRVSGVLFISYPEPWAGAMEE
                     WRGKARALMLDAASDPSIDFVVTYGHRPAYTSQPNNGVVLDVREALDALGDEFGSGVG
                     GKYVLNVAHHVHGEEAFSAQHGVVHLTNGGGGEGQTNLLTADGLVFKSRHPGYLTGDY
                     SATNHQLAVRLRCGPRFGTSNADPCTQGSVLHAVSFTAS"
     gene            2215..>2865
                     /locus_tag="VH419_16960"
     CDS             2215..>2865
                     /locus_tag="VH419_16960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7045364.1"
                     /translation="MTDLRRRRVWAVVPLVAAALVAPLAGPMLTADAATDVTFVAASH
                     SDPASQKFKQAVIPATAQAGDTAVMVFTRATSVSWTGPSGVTGWAQVDSFAGNSLTST
                     VYRKALTAGDVGSTVRFDTANFAKGLLTVSVYRGVDPGSPFVISHASDATSTTSHTTP
                     TVAGVFGQAVMSYWVDRSDTTTAWSTPAPAVTRDTVIGTGTGRYSGLSVDSGGTVAT"
BASE COUNT          461 a          989 c          972 g          443 t
ORIGIN      
        1 gatgtggacc atcgcgatgc cgcccgccga cacgggaggc ggcaaccaac cacccgtcgc
       61 ggctttctcg agcgactgca ccgacctcga ctgcacgttc accgaccact cgaccgactc
      121 cgacggcacg gtgacggcgc acgcgtggca cttcgacgac ggcaccacgt cgaaccagca
      181 gagtcccgcg cacgcgttcc cctcggccgg cagctacgac gtgaccctca cggtcaccga
      241 tgatgccggg gactccgact ccgtgaccca taccgtgaac gtcgacgacg gatcgcagtc
      301 gtcggcgatc tccttcgtcg gcatcggcca ctcggagccg cagtcccaga agttcaagca
      361 ggtcacgatc ccggcgggtg cccaggtcgg tgacgtggcc ttgatggagt tcacccgggc
      421 gagcacggtg gcctggacag gcccgagcca ggtaactggc tggacgcagg tggactcgtt
      481 cactgcctcc ggtctcagct cgaccgtctg gaccaaggtg ctcacggctt ccgatctcgg
      541 cgggacggtc aggttcgaca ccgcgaactt cgcgaaggca ctgcttaccg tgtccgtgta
      601 ctccggtgtc agcgcgggcg tgcccgtctc ggccacccac gcgtcggata cctcacgaac
      661 cgcacacgtg agtccgtcca tcgtcgcttc ccagggggac tgggtcgtct cgtcgtgggt
      721 cgaccggtcc agcacgacga ccgggtgggc ggcgccggga accatgacga gccgcgacgt
      781 ggtgctcagc gacgtcagcg gtcgctacag cggcgtcgtc gcggactccg gcggcccggt
      841 cccggccggg accttcggcg gaaagacggc cacgtcgacg cccgcgacag tcgcgttgat
      901 gtggacggtg aagctcgccg cggggacctc cgctgcggca gacgtggtgg acgagatcaa
      961 ctgcaacttc ggggacgacg acgccagctt gtggatccac taccgcatgc tcgacggtca
     1021 accgaccgag aactccgtgc agtacggcac caccgcggcg tacgggatga cggcgacagc
     1081 ggtcgcgccc ggcatccttc cggtcgaccg ggcgggcgtc ttccgagagg cgcggctgac
     1141 cgggctcagt ccaggccagc tcgtgcacta ccggatcggt cccaacggtc tcgatcacac
     1201 ctgcccgatg ccacccatcg gtgacttccg ctgggacgac gtcggtgaca ccgggacgac
     1261 gctctgcacg ccgtgggtgg cgacgacgca tgcgctgctg gccgccgacg agccggactt
     1321 cgtgacccac ggcggtgacc tctcgtacgc gaacgtctgc ggcactcccg ccgtccacca
     1381 gtactacgtc gaccagcagg tgtggtcgac gaaggcggcg ttccagccga cgtggggcaa
     1441 ccacgagtac ggccagcctg aagacgaggc tccaccgggc actcctcgtg accggctggc
     1501 caactacaag agccgctcca cgatcacgca tgcgcagacc ctggccatcg acgggccgaa
     1561 ccgcctgagc ccacccggtt gcccggccgt ggccggctcg tcgacgaact cctgccgggg
     1621 cgaggactgg ggatggttcc gggtctctgg cgtgctgttc atctcttacc cggagccatg
     1681 ggccggtgcg atggaggagt ggcgcggcaa ggcgcgcgca ctgatgctcg acgccgcgtc
     1741 cgacccgtcc atcgacttcg tcgtgaccta cggccaccgt ccggcataca cgagccaacc
     1801 caacaacggc gtggtgctgg acgtgcggga agcgttggac gccctcggtg acgagttcgg
     1861 ctccggtgtc ggcggcaagt acgtgctcaa cgttgcgcac cacgtgcacg gcgaagaggc
     1921 gttctcggcg cagcacgggg tcgtccacct gaccaacggt ggcggtggcg agggtcagac
     1981 gaacctgctg accgcggacg gcctcgtctt caagagccgg cacccgggat acctgacggg
     2041 cgactactcg gcgaccaacc accagctggc ggtgcggctg cgctgcggac cgcggttcgg
     2101 cacgagcaac gctgatccgt gcacgcaggg aagcgtgctc cacgcggtca gcttcaccgc
     2161 atcctgatcg aagatgtccg gagatcgaca tggtccttga gaggggcgga tgacatgact
     2221 gatttgcgac gacgtcgcgt ctgggcggtg gtgcctctgg tggcggcagc cttggtggca
     2281 ccgctggccg ggccgatgct cacggccgat gccgccacgg atgtcacctt cgtggcggcg
     2341 agtcactccg accctgcctc gcagaagttc aagcaggcgg tcatcccggc cacggcgcag
     2401 gcgggtgaca ccgcggtgat ggtgttcacc cgggcgacgt cggtctcctg gacgggtcct
     2461 tccggcgtca ccgggtgggc ccaggtggac tccttcgccg gcaactcgct gacctcgacg
     2521 gtgtatcgca aggcgttgac ggccggtgat gtcggctcga cggttcgctt cgacaccgcg
     2581 aacttcgcca agggtctgct gacggtgtcg gtataccggg gcgtcgatcc cggcagcccg
     2641 ttcgtgatct cccacgcgtc cgacgccacc tcgacgacca gccacacgac cccgaccgtg
     2701 gcaggggtct tcggccaggc agtgatgtcg tactgggtgg accggtccga cacgacgacg
     2761 gcctggtcca cgccggcccc ggccgtgacg cgggacaccg tgatcggcac cgggaccgga
     2821 cggtacagcg gtctgtccgt cgacagtggc ggaaccgttg ccacg
//
LOCUS       DATBFZ010000557         1572 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_5241713, whole genome shotgun sequence.
ACCESSION   DATBFZ010000557 DATBFZ010000000
VERSION     DATBFZ010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5241713"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            69..380
                     /locus_tag="VH419_16965"
     CDS             69..380
                     /locus_tag="VH419_16965"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY7045365.1"
                     /translation="MAAPKKYPDDLRARAVRLYRESEPKPTIRRLAEQLNVHHEALRN
                     WIRQAEADAGERHDRPTTEMAEENRRLREEVAELRRVNEILRAASSYFASELGPTRRR
                     S"
     gene            377..1291
                     /locus_tag="VH419_16970"
     CDS             377..1291
                     /locus_tag="VH419_16970"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEY7045366.1"
                     /translation="MRFVDEHRDRFAVALLLRVLNIAESTYYQWVKQAEQPCDRDLVD
                     LGLLSNIHEIWEASGRTYGADRVHRQLRRDGIRVGRKRVERLMAGQGWHGAFLRRGWR
                     GGSTRQDPRHTPAPDLVNRNFTAGAPNRLWVADATRIPCGEGVFWLAAVRDVFSRRIV
                     GWKTSDRCDTDLILAALEYGIWSRDVRDGQLIHHSDRGSNYTSFRFSQRLQDNGIRPS
                     MGSVGDSYDNALMENFWSTLKIELVYRTSWRTRDEAENAIFTYIDTWYNARRIQKELG
                     YLSPDEYETTWHAQTQPDNLTPAPTGGR"
BASE COUNT          291 a          518 c          521 g          242 t
ORIGIN      
        1 ggctgagttc cccccggttt ggcggagctt ctcaagcatg atcgactgat catggggagg
       61 aagcatccgt ggccgcaccg aagaagtacc ccgacgacct acgggcgcgt gccgtgcgct
      121 tgtatcgcga gtcggagccg aagccgacga tccggcgcct ggcggagcag ctgaacgtgc
      181 atcacgaggc gctgcgcaac tggatccggc aggccgaggc cgacgcgggt gaacgtcatg
      241 accggccgac caccgagatg gccgaggaga accgccggct gcgggaagag gtcgctgagc
      301 tgcgccgggt taacgagatc ctgcgcgcgg cgtcctcgta tttcgcgtcg gagctcggcc
      361 cgacccggcg acggtcatga ggttcgtcga cgaacaccgt gaccgcttcg cggtcgcgct
      421 cctgctacgg gtcctgaaca tcgccgagtc gacctactac cagtgggtga aacaggccga
      481 acagccctgc gaccgggacc tggtcgacct cgggctgctc tccaacattc atgagatctg
      541 ggaggcgtct ggccgtacct acggcgccga ccgggtccac cggcagttgc gccgcgacgg
      601 gatccgggtg ggccgcaaac gcgtggagcg gctgatggcc ggccagggct ggcacggggc
      661 gttcctgcgc cgcggctggc gcggtggctc aacgcggcag gacccgcggc acacgccggc
      721 gccggatctg gtcaaccgga acttcaccgc aggagcgccg aaccggctgt gggtcgccga
      781 tgccacccgg atcccgtgtg gcgagggcgt gttctggctg gccgccgtcc gggatgtgtt
      841 ctcccgccgg atcgtgggct ggaagacctc cgaccggtgc gacaccgatc tgatcctggc
      901 cgcgctcgag tacggcatct ggtcgcgtga cgtccgcgac ggccagttga tccaccacag
      961 cgacagaggc tcgaactata cgagcttccg cttctcgcaa cgcttgcagg acaacggaat
     1021 ccggccctcg atgggctcgg tcggcgactc gtacgataac gcgctgatgg agaacttctg
     1081 gtcgacgctg aagatcgaac ttgtctatcg cacgagctgg cggacccgtg acgaggccga
     1141 gaacgcgatc ttcacctaca tcgacacctg gtacaacgcc cgccgcattc agaaggaact
     1201 cggctacctc agccccgacg aatacgagac cacctggcac gcccagacac agccagataa
     1261 cctcacccct gccccgaccg gaggcaggta accaccgctc cgccaaagcg ggggtggctc
     1321 acagagccgc agccgatgaa acatggcgaa ggcgaccacg ctgagccgta acatctcctg
     1381 ttccaccaga tccggcatgg ccgagttcgg ttgcccgaga tgcacgcggc gcacctcgcg
     1441 ggcggcgaac tccgccagca aacgttcgta gagttggccg gtgtcgaacg tctcgtcgtc
     1501 gtgttgcaga gcattcccag tggcgtcata gagcgcgagc atcagcagca acagcggctg
     1561 gttagcaaga tc
//
LOCUS       DATBFZ010000558         1591 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_4916811, whole genome shotgun sequence.
ACCESSION   DATBFZ010000558 DATBFZ010000000
VERSION     DATBFZ010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4916811"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="VH419_16975"
     CDS             complement(<1..187)
                     /locus_tag="VH419_16975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7045367.1"
                     /translation="MAIVTNMTSLNAGDLVGRMPGIRSWLVTEPELPGRPGPAVLEPH
                     PETTAVAVTSTAAVRSDR"
     gene            607..879
                     /locus_tag="VH419_16980"
     CDS             607..879
                     /locus_tag="VH419_16980"
                     /inference="COORDINATES: protein motif:HMM:NF020532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1876 domain-containing protein"
                     /protein_id="HEY7045368.1"
                     /translation="MNTAKTWNVHIVLGEHDDGSTYAEARLVPHHEVTLIGVGEARLN
                     PRDRNVPEIGDELAAARALSDLAHRLLDAAAEDISGVAQGFRKPGS"
     gene            891..>1591
                     /locus_tag="VH419_16985"
     CDS             891..>1591
                     /locus_tag="VH419_16985"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY7045369.1"
                     /translation="MTSKRSSRVVVGIDGTAAGLRAAAYATTEAEQRGAELLLVHAHH
                     LPTIMNPSLASLDNEQVRDHGRIALDTAEVGVRYSHPAMQVQTLLVPGRPARAIVDQS
                     AHADLVVVARRERKGSHIVGGRISNAVAARAHCPVVSLNHSWREDHDGRVVVGVDGSP
                     NGRDALAYAFEYASIHHLRLAVLRSWELPLFLQLGEPASAEIDEWTEKAKLALAEDLA
                     GWRSEYPDIQVLPHV"
BASE COUNT          283 a          548 c          498 g          262 t
ORIGIN      
        1 ggcgatcgct ccggacagct gcggtgctgg tgactgccac agccgtcgtt tccgggtgcg
       61 gttcgagcac ggcaggcccc ggccgcccag gcaactccgg ttcggtcacg agccaggaac
      121 gaatgcctgg catgcgaccg accaagtcgc ctgcattcaa tgacgtcatg ttcgtcacga
      181 tggccattcc ggtcgagcgt cgtctcatca cgctcgcagc ccgagctcga ccgggccgga
      241 aggacctgcc ggcgtggttg ctcgccgatg tgcgcaagga cgcgcaatcc cgacttcacg
      301 ccgaggtcgc ccggttccgg gcatgtgacc gggccgacct gtgtgatcgc tgggtacggc
      361 agctgtcttc cgccacctgg cggagccagg atcgctatct tccagcgtgt cggctggcgc
      421 cgcccacccg aaggaaccac gcgcgctcaa cgctgcggtg tgcactcgag accgcttcac
      481 gcctgtctcg ctacgaacag ctcaacgggt tgaacccaga caccagacgg cttgcctcca
      541 ccatgcaccg acagaccacg gatcagctct cccggatgtg tcggaattga cccgagagga
      601 gcagaaatga acaccgcaaa gacctggaac gtccacatcg tcctcggcga gcacgacgac
      661 ggctctacct atgcggaagc ccgtcttgtc ccgcaccacg aggtcaccct gatcggcgtc
      721 ggagaggctc ggctcaatcc gcgtgaccga aacgtcccgg agatcgggga cgagctcgcc
      781 gctgcacgcg ccctgagcga tctggcccac cggttgctgg atgccgccgc cgaagacatc
      841 tcgggggtcg ctcaaggctt ccgaaaaccc ggcagctgag aggaactgtc atgacgtcga
      901 aacgcagttc acgtgtcgtc gtcggtatcg acggcacggc cgcagggctt cgtgccgctg
      961 cgtacgcgac cacggaggcc gagcaacgcg gcgctgagct cctgctggtg cacgcgcatc
     1021 acctccccac gatcatgaat ccatcgcttg cttctctcga caacgagcag gtccgcgacc
     1081 acgggcgcat cgcgctcgac accgcagaag tcggtgtccg gtattcacac ccggcaatgc
     1141 aggtgcagac gctcctcgtt ccggggcgac ccgcccgtgc catcgtcgat caatccgccc
     1201 acgcggacct ggtggtcgtg gcacggcgtg agcgcaaggg cagccacatc gtcggggggc
     1261 gaatctccaa cgccgtcgca gcgcgggctc actgcccggt ggtcagcctc aatcactcat
     1321 ggcgtgagga tcacgacggg cgcgtcgttg tcggggtcga cggatcaccg aatggacgag
     1381 acgcgctcgc gtacgccttc gagtacgcct cgatccacca cctccggctc gctgtgctcc
     1441 gcagctggga acttccactg ttcttgcagc tcggcgaacc cgcctcggcc gagatcgatg
     1501 agtggacgga gaaggccaaa ctggctctgg ctgaggacct ggccgggtgg cggtccgaat
     1561 accctgacat ccaggtgctt cctcacgtcg a
//
LOCUS       DATBFZ010000559         8212 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_4916812, whole genome shotgun sequence.
ACCESSION   DATBFZ010000559 DATBFZ010000000
VERSION     DATBFZ010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 8212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8212
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4916812"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..720
                     /locus_tag="VH419_16990"
     CDS             <1..720
                     /locus_tag="VH419_16990"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017935169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEY7045370.1"
                     /translation="FLIGGDHCTRRCDFCQIDTGKPAPLDRDEPRRVAESVHTMGLRY
                     ATITGVARDDLPDGGAWLYAETVRQIHALNPGTGVENLIPDFNGRPALLAEVFDSRPE
                     VLAHNVETVPRIFKRIRPAFRYDRSLDVITSARDFGLITKSNLILGMGETRAEVSQAL
                     LDLYDAGCDLVTITQYLRPSARHHPVERWVKPEEFIELQQEATDIGFAGVMSGPLVRS
                     SYRAGRLYQQAVASRGAQASA"
     gene            757..1467
                     /locus_tag="VH419_16995"
     CDS             757..1467
                     /locus_tag="VH419_16995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012920297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4191 domain-containing protein"
                     /protein_id="HEY7045371.1"
                     /translation="MARKDKGEKKQSSISKLIQAYKMTRQRDKRVTLVCLLWFLGVGL
                     VFGALFWYFLHPVAGVIVGVATGLLAAMVVFGRRAERAAYAQLEGQPGAAAAALGVLK
                     RGWHVQPAVSVTKNQDLVHRVVGRPGVILVGEGNPQRVRNLLGVEKKKHARVVGEVPI
                     YDIVAGDTGEDAVPIKRLSKHIMKLPRNIRPADVTDLLQRLKALDAMRPQMPLPKGPM
                     PTTARQAKQAARQSMRGR"
     gene            complement(1647..2072)
                     /locus_tag="VH419_17000"
     CDS             complement(1647..2072)
                     /locus_tag="VH419_17000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008357363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEY7045372.1"
                     /translation="MTSEPVDQQYPGQRLGLPPTGPGAVAGWGRRVLALGIDWALSLL
                     AVGAFVGQDVWTGAGGAGWAPLVVFALEVWILTALLGSSAGQLVTGVVVRRTSGEPLD
                     PWRTLARTVLICLVIPPVIYNSDQRGLHDLAVDSVAVRR"
     gene            2187..3608
                     /gene="glnA"
                     /locus_tag="VH419_17005"
     CDS             2187..3608
                     /gene="glnA"
                     /locus_tag="VH419_17005"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004811889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEY7045373.1"
                     /translation="MFADRDELLSFIKDEDVEFVDVRFCDLPGVMQHFTVPVSSFGPA
                     VFDDGLMFDGSSIRGFQAIHESDMALFPDPTTAYLDAFRTDKTLVVNFFIHDPLTGEA
                     YSRDPRNIARKAQEYLKSTGIGDTAYFAPEAEFYVFDNVRFDTRQQEGFYHIDSIAGA
                     WNTGTEGVDARGYKVRYKGGYFPVPPYDHFADLRAEITRELETSGLQVERQHHEVGTA
                     GQAEINFRFDELLACADNLMKFKYIVKNVAWKNGKTATFMPKPIFGDNGSGMHCHQSV
                     WAEGEPLFYDESGYGGLSDTARYYIGGLLAHAPSLLAFTNPTVNSYHRLVPGFEAPVN
                     LVYSQRNRSACIRIPITGANPRAKRIEFRCPDPSANPYLAFSAMLLAGLDGVKNKIEP
                     PAPIDKDLYELPPDEHADVETVPATLTAVLDTLESDHDYLLEGGVFTPDLIETWIDYK
                     RTNEVMPIALRPHPHEFELYYDI"
     gene            complement(3857..4138)
                     /locus_tag="VH419_17010"
     CDS             complement(3857..4138)
                     /locus_tag="VH419_17010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7045374.1"
                     /translation="MIKKKMLRMAIAVAAVAAPLTVLTPNAHASGGDCSYTSSSGEQI
                     SLEDGQQVWERDGDSPNGYFWACQEGTLIFAGYGSEPDWGGYTPGPLEN"
     gene            4618..5310
                     /locus_tag="VH419_17015"
     CDS             4618..5310
                     /locus_tag="VH419_17015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7045375.1"
                     /translation="MAAWAVGGEAFRPPFWQGDGDQNASSATAAQHDGSGSDPAQPVR
                     ARSGMIPKSKPKGVQNFIDACQTRWQEQGRPLDAADDSLSQWRVHVVAMNKLVAGQIT
                     LSQATAFWNQTRLGAIHKVHRFDQSLALFKRSGTPCGRPVGKLVATLAAIDTLDRCVR
                     ATAAGDRVLDVAESAIMTWEHHIHDMEMLRSGQLSPTQATRMWVSNWHKGNRQIDRYA
                     DAEQRARSIHCA"
     gene            5547..5708
                     /locus_tag="VH419_17020"
     CDS             5547..5708
                     /locus_tag="VH419_17020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7045376.1"
                     /translation="MWVLVILLVLVVAAAAYLMFTKGSSTIDEKSLRERHRNIEKEAN
                     LMRRPNYDE"
     gene            complement(6000..7757)
                     /locus_tag="VH419_17025"
     CDS             complement(6000..7757)
                     /locus_tag="VH419_17025"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEY7045377.1"
                     /translation="MTLTLVRLAFSGMRFRLLATGLTVVLACAAAATVVLALQVGETI
                     RDPWQRTFDASHGAHVLVIASSHEQARSLRNAAGVTEAAAPVPYAFSGIRLATPGGPE
                     PVQLLSLSGRPSVNAPVRISGTTTPGSGIVLERSLADALGVGVGTSLRLAGPDAERQL
                     PVVGTAVVPSQGRYPRVNPGIGWVSEPTLRDLEADQARWHWLVAMRLAEPEASAGVAT
                     TILRHAPGSTVSVQTWEDQRNDALKDVEPFRLVLTMYAMVLLGVVFIVVAILVGARAL
                     QQHREIGVLKAVGLSPPQVTSIFVIESTALGLIGSSLGFFVGTLLAPRVAQAAAASLL
                     GSPTVAVDPLALLLAVAPVTLVLAISSWASTRHRTQFSVVQAVEAGRAAPPDESRLGT
                     LVSALPMPAPVVVGTRTVLAGRGRLALLATATCLTGATLVFALSMQASFAAQPAGQVS
                     DVPAELPAVVYTLDAVLVVIALTSLLAVSLLTLRERLRDFAVLKAMGLTPRQVASTLV
                     APYAVMALVSALVSVPLGIALFLAAFYASGSDSDPTLASAAVMAVVPVGMTLLVVVVV
                     AAPARLATRIPAAVALRAE"
     gene            complement(7754..>8212)
                     /locus_tag="VH419_17030"
     CDS             complement(7754..>8212)
                     /locus_tag="VH419_17030"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY7045378.1"
                     /translation="FQFFNLLPALSAGENVELPLLLTGQRRGQARRTAAELLRDLGVA
                     DKQSAAPGRLSGGQQQRVALARALANDPDLILADEPTGNLDSSSTRDVLTLLKAAHQR
                     GRAVLVATHDPRVAAAADRVLTMRDGQITDDRAFADARPVSLPFEQDLRP"
BASE COUNT         1476 a         2743 c         2758 g         1235 t
ORIGIN      
        1 ttcctcatcg gtggcgacca ctgcacccga cgctgcgact tctgccagat cgacacgggc
       61 aagccggcac cgctcgaccg tgacgaaccc cggcgagtcg ccgagagcgt gcacacgatg
      121 gggctgcggt acgccacgat caccggcgtc gcgcgcgacg acctgcctga cggtggcgcg
      181 tggctgtatg ccgagacggt ccggcagatc cacgccctca accccgggac cggcgtcgag
      241 aacctcatcc ccgacttcaa cggcaggccg gccctgctcg cggaggtgtt cgactcccgc
      301 ccggaggtgc tcgcgcacaa tgtcgagacg gtgccgcgta tcttcaagcg gattcgaccg
      361 gccttccggt acgaccgctc gctcgacgtg atcacctcgg cccgcgactt cgggctcatc
      421 acgaagtcga acctgatcct gggcatgggc gagacgcggg cggaggtgtc gcaggcgctg
      481 ttggacctgt acgacgccgg ctgtgacctg gtgaccatca cccagtacct ccggccgtcg
      541 gcgcgccacc accccgtcga gcgatgggtc aagccggagg agttcatcga gctccagcag
      601 gaggcgaccg acatcgggtt cgccggggtg atgagcgggc cgctggtcag gtcgtcgtac
      661 cgggcgggcc ggctctacca gcaggcggta gcatcgcgtg gcgctcaggc gtcggcctga
      721 ccggcatccc agcaacgaac gaggacacca ccagccatgg cacgcaagga caaaggcgag
      781 aagaagcaga gctcgatcag caagctgatc caggcctaca agatgacgcg ccagcgcgac
      841 aagcgggtca ccctcgtctg tctgctgtgg ttcctcgggg tcggtctcgt cttcggtgcg
      901 ctgttctggt acttcctgca cccggttgcc ggtgtcatcg tcggcgtggc gaccggcctg
      961 ctggccgcga tggtggtgtt cgggcgccgg gccgagcggg cggcgtacgc gcagctggaa
     1021 ggccagccag gtgccgcagc tgcggccctg ggcgtgctga agcgtggttg gcacgtgcag
     1081 ccggcggtgt ccgtgaccaa gaaccaggac ctcgtccacc gggtcgtcgg ccggcccggc
     1141 gtgatcctgg tcggtgaggg caacccgcag cgcgtgcgga acctgctcgg ggtcgagaag
     1201 aagaagcacg cccgcgtggt cggcgaggtg ccgatctacg acatcgtcgc cggcgacacc
     1261 ggtgaggacg ccgtgccgat caagcggctg tccaagcaca tcatgaagct tccgcggaac
     1321 atccggcccg ccgacgtcac cgatctgctc cagcggctca aggcgttgga cgcgatgcgg
     1381 ccgcagatgc cgctcccgaa gggtcccatg ccgacgaccg ctcgacaggc caagcaagcc
     1441 gcccgccagt ccatgcgtgg ccgctgacgg cgcccttccc ccatctccat ctctccaccg
     1501 ttcgacctcg cccggctgac cccgactgcc cttgtcggtt tgcgatgttt acagcccgga
     1561 cgccgtcttt ccatcgcaga tccgcgatgt gaagggtggg ggcggcatgt aaacatcgca
     1621 aaccccgtcc cccatccgga cgggtttcag cggcgtacgg cgaccgagtc caccgccagg
     1681 tcgtgcagcc ctcgctggtc ggagttgtag atgaccggcg ggatcaccag acagatcaac
     1741 accgtccgag ccagcgtgcg ccacgggtcg agcggctctc ccgacgtacg ccggacgacg
     1801 actccggtga cgagctgacc cgcagaactc ccgagcaatg ccgtgaggat ccagacctcg
     1861 agcgcaaaga ccaccagcgg cgcccagccc gccccgccgg cgccggtcca cacgtcctgg
     1921 ccgacgaagg cgccgacggc cagcaaggac agcgcccagt cgatgcccag tgcgagcacc
     1981 cgccgacccc atccggcgac cgctccgggg ccggtgggcg gcaggccgag acgctgtccc
     2041 gggtactgct ggtcgaccgg ctccgacgtc acgcgcggca acgtaacacg cgcgaaacac
     2101 ggcagacacg gcagggaaat cgccggctcc taccttgaga ccagaccgga ggccagagcg
     2161 ggccgagccg gatgcgagga ggatcgatgt tcgccgaccg cgacgagttg ttgagcttca
     2221 tcaaggacga ggacgtcgag ttcgtcgacg tccggttttg cgacctgccg ggggtgatgc
     2281 agcacttcac cgtgccggtg tcgtcgttcg ggccggcggt gttcgacgac gggctgatgt
     2341 tcgacggctc gtcgatccgc ggcttccagg cgatccacga atccgacatg gcgctcttcc
     2401 ccgaccccac gacggcgtac ctcgacgcgt tccgcaccga caagacgctc gtcgtgaact
     2461 tcttcatcca cgacccgctc accggagagg cgtacagccg cgacccgcgc aacatcgccc
     2521 gcaaggcgca ggagtacctc aagagcaccg gcatcggcga cacggcgtac ttcgctcccg
     2581 aggcggagtt ctatgtgttc gacaacgtgc ggttcgacac ccggcagcag gagggcttct
     2641 accacatcga ctcgatcgcc ggcgcctgga acaccggcac cgagggcgtc gacgcgcgtg
     2701 gctacaaggt ccgctacaag ggcggctact tcccggtacc cccgtacgac cacttcgccg
     2761 atctccgtgc cgagatcacc cgcgagctcg agacgtccgg cctgcaggtc gagcggcagc
     2821 accacgaggt cggcaccgcc ggccaggccg agatcaactt ccgtttcgac gagctgctcg
     2881 cctgcgcgga caacctgatg aagttcaagt acatcgtcaa gaacgtcgcg tggaagaacg
     2941 gcaagacggc gacgttcatg ccgaagccga tcttcggcga caacggctcc ggcatgcact
     3001 gtcaccagtc ggtgtgggcg gaaggggagc cgctgttcta tgacgagagc gggtacggcg
     3061 gtctgagcga cacggcgcgg tactacatcg gtggacttct cgcgcatgcg ccgtcgctgc
     3121 tcgccttcac caacccgacc gtgaactcct accatcgcct cgtgcccggc ttcgaggctc
     3181 cggtcaacct cgtctacagc cagcgcaacc ggtcggcctg catccggatc ccgatcaccg
     3241 gcgcgaaccc ccgcgcgaaa cggatcgagt tccgttgccc cgacccgtcg gcgaacccgt
     3301 acctggcgtt ctccgcgatg ctgctggccg gtctcgacgg ggtgaagaac aagatcgagc
     3361 cgccggcgcc catcgacaag gacctgtacg agcttccgcc agacgagcac gccgacgtcg
     3421 agaccgtccc ggccacattg accgccgtac tcgacacgtt ggagtccgac cacgactacc
     3481 tgctcgaagg cggtgtgttc accccagacc tcatcgagac atggatcgac tacaagcgca
     3541 ccaacgaggt catgcccatc gcccttcgtc cccaccccca cgaattcgag ctctactacg
     3601 acatctgatc tggaattggt cgtggagagg ccggttgggc gctgccgtct gacagtgtcg
     3661 aggtgcgagc gggtgcttga ctggaacggc gcgtgcggtg cacggcaccg agcaagccgt
     3721 cctccgtccc ggcaagcccc cgtcgaccgg actcccgacg tactgagcct gccgaggtgt
     3781 caccggcgtg ccgaaacccg acaacgatgg acgaaagttg gcacctcggc ggtgtgggta
     3841 gcgcggagtg tcgttgtcag ttctcgagtg ggccgggcgt gtagcctccc cagtcgggct
     3901 cggagccgta gccggcgaag atcaaggttc cctcctgaca cgcccagaag tagccgttgg
     3961 gagagtcacc gtcccgttcc cagacttgtt ggccgtcctc gagcgagatc tgctcgcctg
     4021 aactgctcgt gtagctgcag tctccgccgg aggcatgtgc gttcggcgtg aggaccgtga
     4081 gaggcgcagc gactgccgca accgcgatcg ccatccgcag catcttcttc ttgatcatcg
     4141 ttccccctac tgctgtccgt gattcgtttc tggacgcacc gcatcccgca cgtgattcgc
     4201 tgccggaccg taccccggat gccgccgcgg tgttgatcag gagtcggcgg cgaacttcgg
     4261 tacgcgtctg tcgacgacag cgtcggaccg accggtaggc agcgacggcc ccacgtttgc
     4321 acgcgggctg gcgctgcttc gtccagacgc tccgagtgcg caattgaccg cagctcgcac
     4381 cgaggtgtcg ccccctgtcg acacgccgcc gaagatgggc gacagttgac acatcggcgt
     4441 ttcgcggagt cgtcggacgg gcaccaacga gggaacatgt aaacaacatg tgggtcaggg
     4501 gggtctgaca tggggtacga cggtgcagac gagggaagtt attcttcgag accgggtaag
     4561 cggcgggcgg attggatcgc catcgtggcg ctgtgcgttc tcgcattact cgcaaatgtg
     4621 gcagcctggg cggtcggtgg cgaggcgttc cgcccgccgt tctggcaggg ggacggcgac
     4681 cagaatgcct cgagcgcgac agcagcgcag cacgacggca gcgggagtga tccggcccaa
     4741 ccggtgcgcg cccgcagtgg catgattccg aagtcgaagc cgaaaggagt tcagaacttc
     4801 atcgacgcct gccagactcg ttggcaggag caggggcgac cactcgatgc cgccgacgac
     4861 tccctcagcc aatggcgagt ccatgtggtc gcgatgaaca agctcgtggc cggccagatc
     4921 acattgtcac aggcgaccgc gttctggaac cagacccgac tcggtgcgat ccacaaggtg
     4981 caccggttcg accagagcct tgcgttgttc aagcgcagcg gcacaccgtg cggacgtccg
     5041 gtcgggaagc tcgtcgccac cctcgcggcg atcgacacgc tggatcggtg cgtgcgtgcg
     5101 accgcggccg gagaccgtgt cctcgatgtc gccgaaagcg ccatcatgac gtgggagcat
     5161 cacatccacg acatggagat gctccgcagc ggccagctct cgccgactca agccacccgg
     5221 atgtgggtga gcaactggca caagggaaac cggcagatcg acaggtacgc cgacgccgag
     5281 cagcgggcca ggagcatcca ctgcgcctga cccgcgaaga catcaggcga ggtcaacggc
     5341 ggtccgaccg ccgacatact tgggcgaacg ttggcacctc ggcggaagca agagggtcag
     5401 ccggcgcgag cgggggcagc gtacggcatc caatcgctga ggtaccgggc cgtgccgttc
     5461 ctgtgtcccg gcgcatcgag tcgctgtctt gacagggatt caggcgcacg cgcacagtca
     5521 aatgaggaga gcgaggtggt ggttcgatgt gggtcctcgt cattcttctc gtgttggttg
     5581 tcgccgccgc tgcttacctc atgttcacga agggttcctc cacgattgac gagaagagtt
     5641 tgcgcgaacg gcaccggaac atcgagaagg aagccaacct gatgagacgg ccgaactacg
     5701 acgagtgacg acgctgcgag acaatccgac atcttgaaac gggtgcgagc gaatcggatc
     5761 atccaattcg accgacccat gacagctcgt caacacaccg tgaagttggt caaccaagcc
     5821 accggcgccc atccacgcat cgacctcgac gcagcgatcg tcaacgaggt ctgctggacc
     5881 gtcatcagta ttccgccgcg gtgttgatca ggtcgtcgaa ccgaacgtca gagcgcggac
     5941 tgtcgggcca tgacacgtcc ccggaaccgc ccagatccct ggcggttcgg cgttcgaaat
     6001 cactccgcgc gcagagcgac ggctgccggt atgcgagtgg ccagccgggc cggcgcagca
     6061 acgacgacga ccaccaggag cgtcatgccg acgggcacaa cggccatcac tgcggccgag
     6121 gcaagggtgg ggtcactgtc gctaccggat gcgtagaacg ccgccaggaa caacgcgatg
     6181 ccgagcggca ccgacacaag cgcggacacc aaggccatga cggcgtacgg cgcgacgagg
     6241 gtcgaggcga cctgtcgtgg cgtcagaccc atcgctttga ggaccgcgaa gtcccgcagc
     6301 cgttcccgca gcgtcagcag tgagacggcc agcagggagg tcagggcgat gacgacgagg
     6361 acggcgtcga gggtgtagac aacggcgggc agctcggctg gaacatcgct cacctgtccg
     6421 gctggctgcg ccgcgaacga tgcctgcatc gacagcgcga acacgagggt ggcgccggtg
     6481 aggcaggtgg cggtggccag cagggcgagc cgcccgcgtc cggccagcac tgtgcgtgtg
     6541 ccgacgacca caggggcggg catcggcaga gcgctgacca gagtgcccaa tcgcgactcg
     6601 tcaggcggcg cagcacgccc cgcctcgacc gcctggacaa cggagaactg cgtacgatgt
     6661 cgagtcgatg cccatgagct gatggcgagg acgagcgtga ccggcgcgac cgccaagagc
     6721 agggcgagcg ggtcgactgc caccgtcggc gaccccagca acgacgccgc cgcggcctgg
     6781 gccacccgag gcgccagcag ggtgccgacg aagaagccga gactcgaccc gatcagcccg
     6841 agtgcggtgg actcgatcac gaagatcgac gtcacctgtg gcggcgacag accgactgcc
     6901 ttgagtacgc cgatctcgcg atgctgctgc agcgcccgcg ctccgacgag gatggcgacg
     6961 acgatgaaca cgacgccgag caggaccatc gcgtacatcg tcagcaccag ccggaacggc
     7021 tcgacgtcct tgagcgcatc attgcgctgg tcctcccacg tctgcaccga gaccgtgctc
     7081 cctggcgcat gtcgaaggat ggtcgtcgcg acaccggcgg atgcttcggg ttcggcgagc
     7141 cgcatcgcca ccagccagtg ccagcgggct tggtcggcct cgagatcccg cagggtcggc
     7201 tcgctgaccc aaccgatgcc ggggttgacc cggggatacc gaccctgact cgggaccacc
     7261 gccgtaccga cgacgggcag ctgcctctcc gcgtccgggc cggccaaccg gagcgacgtg
     7321 ccaaccccaa cgcccagcgc gtcggccagg ctgcgttcga gcacgattcc gctaccgggc
     7381 gtcgtcgttc cgctgatgcg gaccggggcg ttgacgctgg gccggccgga caagctgagc
     7441 agctgcacgg gttccgggcc gccgggtgtc gcgaggcgta tgccggaaaa ggcatagggc
     7501 accggggcgg cagcttcggt gacaccggct gcgttgcgga gtgagcgcgc ctgctcgtgg
     7561 ctcgacgcga tgacgaggac atgcgcgccg tgggacgcgt cgaaggtgcg ctgccacgga
     7621 tcgcggatcg tctcaccgac ctggagggcg aggacgaccg ttgcagccgc ggcgcaggcg
     7681 aggacgaccg tcaggccagt ggcgagcaac cggaagcgca tgccggagaa ggccaacctg
     7741 acgagcgtca gagtcatggg cggagatctt gctcgaacgg caggctgact gggcgggcgt
     7801 ccgcaaaggc ccggtcgtcg gtgatctggc cgtcgcgcat ggtgagaacc cggtctgccg
     7861 ccgcggcgac cctggggtcg tgggtggcca cgagcaccgc gcgtccgcgt tggtgtgcag
     7921 ccttgagcaa ggtcaacacg tcgcgggtgg acgacgagtc gaggttgccc gtgggttcgt
     7981 cggcgaggat caggtcggga tcgttggcaa gcgcccgagc cagcgcgact ctctgctgct
     8041 ggccacctga cagccgtccc ggtgccgcgc tctgcttgtc agcgacaccg aggtcgcgta
     8101 gcagctccgc cgcggttcga cgtgcctgcc cacgccgttg accggtgagc aggagcggca
     8161 gttcgacgtt ctcccccgct gacagcgccg ggagcaggtt gaagaactgg aa
//
LOCUS       DATBFZ010000560         1946 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_6231716, whole genome shotgun sequence.
ACCESSION   DATBFZ010000560 DATBFZ010000000
VERSION     DATBFZ010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6231716"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="VH419_17035"
     CDS             complement(<1..273)
                     /locus_tag="VH419_17035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020391090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEY7045379.1"
                     /translation="MGIVVQKYGGSSVADAQGIKRVAQRIVATRKAGHDVCVVVSAMG
                     DTTDELLDLAYQVSPLPPGRELDILLTAGERISMALLAMAIHNLGYE"
     gene            complement(340..927)
                     /locus_tag="VH419_17040"
     CDS             complement(340..927)
                     /locus_tag="VH419_17040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011753815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5063 domain-containing protein"
                     /protein_id="HEY7045380.1"
                     /translation="MTTDSRGTLSSEMEDLAQGIADQAESFLLAVREIARSGDPGPAV
                     PLLLLEVSQLLLAGARLGVQMDFVPTEPWEPDAGPDPDLDEMRERLAALLEGVDDFTE
                     VFDPYAHPPELISSRLSDDLADIAANVAHGLQHYRAGSTQEALWWWQFSYVSSWGAEA
                     SASLRALQSVIAHDMLDAAEVGEVEASRVAAAQDL"
     gene            complement(917..1516)
                     /gene="recR"
                     /locus_tag="VH419_17045"
     CDS             complement(917..1516)
                     /gene="recR"
                     /locus_tag="VH419_17045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020391097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEY7045381.1"
                     /translation="MYEGIVQDLIDELGRLPGVGPKSAQRIAFHLLNADAVDVQRLAN
                     VLVEVKDKVRFCVTCGNVAEEEQCKICRDPRRDLSVLCVVEEPKDVVAIERTREFRGR
                     YHVLGGAISPIEGIGPDELRIRELMTRLADGAVTEVILATDPNLEGEATATYLTRLLK
                     PMGLHVTRLASGLPVGGDLEYADEVTLGRAFEGRRTVDD"
     gene            complement(1598..>1946)
                     /locus_tag="VH419_17050"
     CDS             complement(1598..>1946)
                     /locus_tag="VH419_17050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019513638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEY7045382.1"
                     /translation="ALLEQAAAMQQQLMAAQEDLAARRVTGSAGGELVRATVTGTGEL
                     VGLKIHPDACDPADPETLADLVLAAVRDAATRAHALAAEQMGGLTGDFGGGLPSGGET
                     DDSPGPGPAGFSS"
BASE COUNT          291 a          701 c          644 g          310 t
ORIGIN      
        1 ctcgtagccc aggttgtgga tggccatggc cagcagggcc atggagatgc gctcgccggc
       61 ggtgagcagg atgtccagct cgcggccggg cggcagggga ctgacctggt aggccaggtc
      121 gagcagctcg tcggtggtgt cgcccatcgc agagacgacc acacacacgt catggcctgc
      181 cttccgggtc gccacgatgc gctgggccac ccgcttgatg ccttgggcgt cggcgacaga
      241 cgacccgccg tacttctgca ccacgatccc cacgccgccc gactcctctc tctcgttgaa
      301 ccgtgagttg tggttcgtcg aaccgggtgt tgtggttggt cagaggtcct gggcagcggc
      361 gacgcgagat gcctccacct cacccacctc ggcggcgtcc agcatgtcgt gcgcgatcac
      421 ggactgcagg gcccgcagcg aggcgctggc ctcagcaccc cagctggaga cgtaggagaa
      481 ctgccaccac cacagcgcct cctgcgtcga gcctgcacgg tagtgctgga gtccgtgtgc
      541 aacgttggcg gcgatgtctg ccaggtcgtc ggaaaggcgt gaggagatca gctccggcgg
      601 atgtgcgtac gggtcgaaca cctcggtgaa gtcgtcgacg ccttcgagca gggcggccag
      661 ccgctcgcgc atctcgtcca ggtcgggatc gggacctgcg tcgggctccc acggctcggt
      721 cggcacgaaa tccatctgta cgccgagccg cgcccccgcg agcaacagct gcgacacctc
      781 gagaaggagc agcgggacgg caggaccggg gtcgccgctg cgagcgatct cgcgcaccgc
      841 caggaggaag ctctcggcct ggtcggcaat cccctgggcg agatcctcca tctcgctcga
      901 cagcgtgcca cgtgaatcag tcgtcaacgg tgcgtctccc ttcgaaggcg cgtcccagcg
      961 tcacctcgtc tgcgtactcc aaatctcccc cgaccggcag tccgctcgca agtcgcgtca
     1021 cgtgcaggcc catcggcttg agcagccggg tgaggtaggt ggcggtcgcc tcgccttcca
     1081 gattcgggtc ggtcgccaag atcacctcgg tgaccgcgcc gtcggccaac cgagtcatca
     1141 gctctcggat ccggagctcg tcgggaccga tcccttcgat ggggctgatc gccccgccaa
     1201 ggacgtgata ccggccgcga aactcccgcg tccgctcgat cgcgaccacg tccttgggct
     1261 cctccacgac gcacagcacg ctgaggtcgc gccgtgggtc ccggcagatc ttgcactgct
     1321 cctcctcagc gacgttgccg cacgtgacgc agaaacggac cttgtccttc acctcgacca
     1381 acacgttcgc cagccgctgt acgtcgaccg cgtccgcgtt gagcaggtgg aacgcaatcc
     1441 gttgcgcgct cttcgggccg acgccgggaa gcctgccgag ctcgtcgatc aggtcctgga
     1501 cgatgccctc gtacactgcg ctcccgttcg tcgacgtcgt cgcgccgtct ttccgggcgc
     1561 cgccagcagc ctagcgagcg tacgacgcgg ggccgggtca ggaggagaac cccgccggac
     1621 cagggccggg cgagtcgtcg gtctcgcctc ccgagggcag cccaccgccg aagtcgccgg
     1681 tcagcccgcc catctgctcg gccgccaacg catgcgccct ggtggcggca tcgcgcacgg
     1741 cggccagcac gaggtcggcg agggtctcgg ggtccgccgg gtcgcaggcg tccgggtgga
     1801 tcttgagtcc gaccagctcg ccggtgccgg tcaccgtggc ccggacaagc tccccgccgg
     1861 cgcttccggt gacacgccgc gccgccaggt cctcctgcgc cgccatcaac tgctgctgca
     1921 tcgccgccgc ctgctcgagc aaggcg
//
LOCUS       DATBFZ010000561         3792 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_4592401, whole genome shotgun sequence.
ACCESSION   DATBFZ010000561 DATBFZ010000000
VERSION     DATBFZ010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 3792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3792
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4592401"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="VH419_17055"
     CDS             complement(<1..587)
                     /locus_tag="VH419_17055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020664821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEY7045383.1"
                     /translation="MKLAGVELRRITMPLVAPFRTSFGTQTVRDVLLLRVVSDKAEGW
                     GECVASSDPLYSSEYVDAAADVLRRFFVPALAATGSFDAPGVAGVLARFKGHRMAKAA
                     LEMGVLDAELRAEGRSFARELGAVRDRVPCGVSVGIMDTIPALLDAVGAYLDEGYLRI
                     KLKIEPGWDVDPVRAVRERFGDDLLLQVDANTAYTL"
     gene            complement(584..1462)
                     /locus_tag="VH419_17060"
     CDS             complement(584..1462)
                     /locus_tag="VH419_17060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020392848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY7045384.1"
                     /translation="MDELTELSDVEGDLVQAVQTAGAAALAAGVSVRNVEDLAELEQV
                     VTLFASIWGRDANPPMTLELLRAFTKAGNYVGGAFEGQHLVGACVGFFHAPAEDALHS
                     HIAGVAAGMTGRNIGFALKLHQRSWAMVRGVSEIAWTFDPLVSRNAYFNLSKLCAQPV
                     EYLPNFYGPMLDTINGVDDSDRLLVRWRLRDPSVVAACAGRGKPTLLADLLAAGATVG
                     LDVGDDGAPKWGSLEGETVLVTVPQDIGALRDHDPAVAAQWRAAVREALAALLADGAC
                     IDGFDRTGSYVVRRHR"
     gene            complement(1486..2619)
                     /locus_tag="VH419_17065"
     CDS             complement(1486..2619)
                     /locus_tag="VH419_17065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020392849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEY7045385.1"
                     /translation="MDLALLLADVRELVECESPSEDLAAVARSADVVARIGTAHLGVA
                     PEYLVRDGRTHLRWRLGDGESRVLLVGHHDTVWPLGSLAAHPWSVEEGIMRGPGCFDM
                     KTGLAMAFHAVAALDRRDGMTVLVTGDEELGSPGSRTLIEEEARLVSAALVLEASAGG
                     GALKTERKGVSLYDVRVIGRAAHAGLEPELGVNATVELAHQSLAVAALSDPAAGTTVT
                     PTAARSGTTSNTVPAVASFSVDVRARSVAEQARVDAGMRALRPVLPGATLEVTGGPNR
                     PPLEASASEALLERARAVARRHGLAEPVGTAVGGASDGNFTAGVGTATLDGLGAIGGG
                     AHAEDEHVLVDALPARAALLEALVADLLAHPISLVESVSGARP"
     gene            complement(2619..>3792)
                     /locus_tag="VH419_17070"
     CDS             complement(2619..>3792)
                     /locus_tag="VH419_17070"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012922486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEY7045386.1"
                     /translation="KVWTATVVMQLVDEGLVELDAPVAEVLPELRLSDPDVAKRVTVR
                     HLLTHTSGIDGDIFTDTGRGDDCLEKYVDLLAEAAQNHPIGATWSYCNAGFVLLGRLI
                     EKLTDSTWDQAMRDRLFTPLGLTRTVTLPEEAILHPVAVGHIDKDGEQILTPAWGLPR
                     SVGPAGLVTSTAADVLAFARLHLTGGLAADGTRVLSEASATAMAAHEADLPDKYVLGD
                     SWGLGWIRFGWDGERLIGHDGNTLGQAAFLRILPEQGLAVTLLTNGGHTRDLYEDLYR
                     ELFAELAGVAMPHPLTPPEQPVEVDVTPFVGTYERASVRMEVLADGPTLRTTVTGPLA
                     EVMPDPVETYPMTPVGPALFAVKPPEAETWFPVTFYELPTGERYVHFGARATPRVD"
BASE COUNT          585 a         1349 c         1329 g          529 t
ORIGIN      
        1 agggtgtacg ccgtgttggc gtcgacctgc agcaggaggt cgtcgccgaa gcgttcccga
       61 acggcgcgca ccgggtcgac gtcccagccg ggttcgatct tcagcttgat ccggaggtac
      121 ccctcgtcga ggtacgctcc gacggcgtcg agcagcgccg ggatggtgtc catgatcccg
      181 acggagactc cgcaagggac ccgatcccgg acagctccca gctcacgtgc gaacgagcga
      241 ccctcggccc ggagctcggc gtccagtacg cccatctcca gcgccgcctt cgccatccgg
      301 tggcccttga accgcgccaa tacgccggcg actcccgggg cgtcgaagga cccggtcgca
      361 gccagcgccg ggacgaagaa ccggcgcagt acgtcggcgg ccgcatcgac gtactccgag
      421 gagtagagcg ggtcggacga cgcgacgcac tcgccccacc cctccgcctt gtcggagacg
      481 acacggagca gcaggacgtc ccgcacggtc tgcgtgccga acgacgtccg gaacggtgca
      541 acgagcggca tcgtgatccg gcgcagctcc accccggcca gcttcatcgg tgtctcctca
      601 cgacgtacga acccgtccga tcgaatccgt cgatgcaggc gccgtccgcg agcagtgccg
      661 cgagcgcctc gcgtaccgcg gcccgccact gtgcggcaac ggccgggtcg tggtcgcgga
      721 gcgcaccgat gtcctgtgga acggtgacca ggacggtctc gccttccagg ctcccccact
      781 tcggcgcacc gtcgtcacca acgtcgagcc cgacggttgc tcccgcagcg agcaggtcgg
      841 cgaggagcgt cggcttgccc cgcccggcgc aggctgcaac gaccgacgga tcccggaggc
      901 gccaccgcac cagcagtcgg tcggagtcgt cgacaccatt gatggtgtcg agcatcgggc
      961 cgtagaagtt cggcaggtac tcgaccggct gggcgcacag cttggacagg ttgaagtatg
     1021 cgttgcgact gacgagcggg tcgaaggtcc acgcgatctc cgagacccca cgcaccatcg
     1081 cccacgaccg ttggtgcagc ttgagcgcga agccgatgtt gcggccggtc atgcccgccg
     1141 ccaccccggc gatgtggctg tgcagcgcgt cctcggccgg ggcgtggaag aacccgacgc
     1201 aggcgccgac caggtgctgg ccttcgaagg ccccgccgac gtagttgccg gccttcgtga
     1261 aggcgcgcaa cagctcgagc gtcatcggag ggttggcgtc gcgcccccag atcgacgcga
     1321 acagcgtcac gacctgctcg agctcggcca ggtcctcgac gttgcgcacc gagacgccgg
     1381 cggccagggc tgccgcgccc gccgtctgaa ccgcctggac caggtcgccc tcgacatcgg
     1441 acagctccgt cagctcgtcc atgctgctga ggttctccgg gctcgtcatg gtcgggcgcc
     1501 actcacgctc tcgacaaggc tgatcgggtg ggccagcaga tccgcaacca gcgcttccag
     1561 cagcgcggcg cgggcgggca gcgcatcgac gaggacgtgt tcgtcctcgg cgtgcgctcc
     1621 acccccgatg gcgccgaggc cgtcgagcgt ggcggtgccg accccggcgg tgaagttgcc
     1681 gtccgacgcg ccgccgacgg cggtgccgac gggttcggcg aggccgtgcc gacgggcaac
     1741 cgctcgggcg cgttccaaca acgcttcgga cgcggatgcc tcgaggggcg gacggttcgg
     1801 accgccggtg acctccaggg tcgcgcctgg caacacgggg cgcagcgcac gcatgcccgc
     1861 gtctacacgg gcctgctcgg caaccgaccg ggcccgtacg tcgacggaga acgaggccac
     1921 ggcggggaca gtgttgctgg tggtgcctga ccgcgccgcg gtcggagtga cggtggtgcc
     1981 ggcagcggga tcgctgagcg cggccaccgc cagcgactga tgggccagct cgacggtggc
     2041 gttgacgccg agctcgggct cgagtccggc atgcgcagcc cggccgatga cgcgcacgtc
     2101 gtacaacgac acacccttgc gctcggtctt gagtgcacca ccaccggctg aggcctcgag
     2161 cacgagtgct gccgagacga gcctggcctc ctcctcgatc aaggtgcgcg aacccggcga
     2221 gccgagttcc tcgtcaccgg tcaccagcac ggtcatcccg tcgcggcgat ccagcgccgc
     2281 gacggcgtgg aacgccatcg ccaggccggt cttcatgtcg aagcagccgg ggccgcgcat
     2341 gatcccttcc tcgacggacc acggatgggc ggccagcgaa cccagcggcc agaccgtgtc
     2401 gtggtggccc acgaggagca cgcgcgactc cccgtcgccc agccgccagc gcaggtgcgt
     2461 ccgaccgtcg cggacaaggt attccggggc caccccgagg tgagcggtgc cgatccgggc
     2521 gacgacgtct gccgaccgag ccacggcggc caggtcctcc gacggcgact cgcactcgac
     2581 gagctcacgc acgtcagcga gcagcagggc gaggtccatc agtccacccg gggtgtggcg
     2641 cgggcgccga agtgcacgta gcgctcgccg gtcggcagct cgtagaacgt caccgggaac
     2701 caggtctcgg cctcaggagg cttgaccgcg aacagtgccg ggccgaccgg agtcatcggg
     2761 taggtctcga ccgggtccgg catcacttcg gccagcggcc cggtgaccgt ggtgcgcagg
     2821 gtcgggccgt ccgcgagcac ctccatccgg accgacgccc gctcgtaggt cccgacgaac
     2881 ggcgtcacgt cgacctcgac cggctgctcg ggtggcgtca gcggatgtgg catcgcgaca
     2941 ccggcgagct cggcgaacag ctcccggtag aggtcctcgt agaggtcgcg ggtgtggcca
     3001 ccgttggtca gcagcgtgac ggcgagcccc tgctcgggca ggatccgcag gaatgcggcc
     3061 tggcccaggg tgttgccgtc gtgaccgatc agccgttcac cgtcccagcc gaaccggatc
     3121 cagcccagcc cccaggagtc gccgaggacg tacttgtccg gcaggtccgc ctcgtgcgcg
     3181 gccatggcgg tcgccgacgc ctccgagagc actcgcgtgc cgtccgccgc caacccgccg
     3241 gtcaggtgca accgggcgaa ggcgaggacg tcggcggccg tggaggtgac gaggccggcc
     3301 ggtccgaccg agcgcggcaa cccccatgcg ggtgtcagga tctgctcgcc gtccttgtcg
     3361 atgtgaccga cggcgaccgg gtgcaggatc gcctcctccg gcaacgtgac cgttcgggtg
     3421 agcccgagcg gggtgaacag ccggtcgcgc atcgcctggt cccacgtcga gtcggtcagc
     3481 ttctcgatca gccggccgag caacacgaag ccggcgttgc agtacgacca ggtcgcgccg
     3541 atcggatggt tctgggccgc ctcggcgagc aggtcgacgt acttctccag gcagtcgtcc
     3601 ccgcgaccgg tgtcggtgaa gatgtctccg tcgatcccgc tggtgtgggt caacaggtgg
     3661 cggacggtta cgcgcttggc gacgtcgggg tcggacagtc tcagctccgg aagcacctcg
     3721 gcgaccgggg catccagctc gaccagaccc tcgtcgacga gctgcatcac gactgtggcc
     3781 gtccagacct tg
//
LOCUS       DATBFZ010000562         7074 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_4593117, whole genome shotgun sequence.
ACCESSION   DATBFZ010000562 DATBFZ010000000
VERSION     DATBFZ010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 7074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7074
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4593117"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1288
                     /locus_tag="VH419_17075"
     CDS             <1..1288
                     /locus_tag="VH419_17075"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY7045387.1"
                     /translation="LVDIADIARSFELPVGTTPTAADLLLDALVVLFTEPLASSIPVV
                     QRALTAIREDPELRNTPRHLARACYLPQALSDDESHFALASACATACRAQGSFQGLNE
                     ALAYLFIRELRVGSIDRAADLVLEQRELRAVVGHPSGLTDVQDLIVSAWRGRETDVRT
                     KAPDLDTVAPPSGYLSRLITHARIVLEVGLGNYDAAASLVRDDWYQDFALGALRAADA
                     TEAQVRSGDVAAALTSAAFLRERASATESPFDRGLWRRSEALLADGGDAETSFRDSIT
                     DLQTCGAQLHVARSQLVYGEWLRRQKRRRDAREVLETARESFEEMGAQAFAERARVEL
                     LATGANARRRVDETRQVLTPQESQIARLAGGGFTNPEIAERLFISANTVDYHLRKVYR
                     KLDIGSRHEIAAALDDRRIERDDAPANLTIAPQIVS"
     gene            complement(1294..2100)
                     /locus_tag="VH419_17080"
     CDS             complement(1294..2100)
                     /locus_tag="VH419_17080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020388494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY7045388.1"
                     /translation="MTRPFRFGVVAPVTPDPATWRDRVRRIADSGFSTLLMPDVPQWQ
                     PAPGPTLAFAAALADLRVGTWVYASPLHPAWSTAWEAHSLSVLTDGRFEMGIGTGRPG
                     IEDELRELGLPVVPSGERLAQVRETVAALRHLDGPDLHTPVVMAVRGPKAQALAAEIA
                     DTVTFAVLPDDPPSEVAQRARDFRRIRDVELALHVPVVGDTVAPFMAPPDTDPAALRA
                     AGSLAILPGDPAAAAEEIQRRREEIGYSYFVFGADAAETLAPVVAELAGH"
     gene            complement(2097..2825)
                     /locus_tag="VH419_17085"
     CDS             complement(2097..2825)
                     /locus_tag="VH419_17085"
                     /inference="COORDINATES: protein motif:HMM:NF017771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 family protein"
                     /protein_id="HEY7045389.1"
                     /translation="MTVARPPVVTQAEWDAALATMTEREKAVATAMNELAAARKRMPM
                     VRVEHQYRFEGPDGERSLPELFDGRSQLILYRFFFEEGVGGWPDAGCVGCSSFADGIP
                     ELGLLHARDITFAMASPAPQANLRRYAERMGWTDVPWYTICTERFSSDFGVEEWFGLN
                     VFLRDGDDVYRTYFLQHGPMVQKIGSVWSLFSLTPYGGQGEDEDVPEGWPQAPLPFWY
                     RRHDEFDETPPSARGIAPARGVKA"
     gene            complement(2822..3403)
                     /locus_tag="VH419_17090"
     CDS             complement(2822..3403)
                     /locus_tag="VH419_17090"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEY7045390.1"
                     /translation="MPELLVDFITSLDGYGAAEGWRGWWGLEGPEYLGWLGGQPEGEY
                     TILMGATTYRIMSGFAASGEPGTDALGGMLKVVFSSTLKEPLSWPNTQLVDRDAVDAV
                     RKMKDEGSTSMRTIGSLTLCRSLLNAGLVDRFRVVVFPVITGSTGRDRIYDGYPDVAL
                     DMISSRTFDGRSQLLEYVPTVLAGPPDTTKAQP"
     gene            complement(3529..4749)
                     /locus_tag="VH419_17095"
     CDS             complement(3529..4749)
                     /locus_tag="VH419_17095"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEY7045391.1"
                     /translation="MTACDVVVVGGGIGGAALAYGLAQSGLGVVVLEASKEFPDRVRG
                     ESMQLWGLAEARRLGVEDVLLKAGAHIAPVWRQYVEGIGEPGDIPASMLLPDIPGTLN
                     LRHPDACQALLDAAAGAGATVVRGVADVTLTAGPEPAVSYRSDGDAATVKTTLVVGAD
                     GRASTIRRQAGIELHRQEPINYIAGLLVDGLDGVPDDYDVLVSEGDYFSLMFHQGGGR
                     ARLYLCGGRSMQHRFAGQDGVAKFMAAWNPSCYPETERVARATPAGPCATYPGDDTWT
                     DAPAAPGVVLIGDAAGHNDPIIGQGLSIAMRDARMVRDLVVDGARQPADFAPYATERV
                     DRMRRLRLAADIMSVVQAEDCDNLMARRQLMAEKMAAMDPEFFPVLIGCFTGPETIPE
                     ECVDDRVLDRLRRA"
     gene            complement(4787..5083)
                     /locus_tag="VH419_17100"
     CDS             complement(4787..5083)
                     /locus_tag="VH419_17100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7045392.1"
                     /translation="MFIQVNITHSDHSIEEFTALEDEFVKLTEGERTIQRAIGCVGAD
                     DPSLRVLIAIFPDAEAAARNSALPGTDQVAQKVMAMSKGEPQFINLNVVTDRSF"
     gene            5413..5679
                     /locus_tag="VH419_17105"
     CDS             5413..5679
                     /locus_tag="VH419_17105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7045393.1"
                     /translation="MDRPRDPDLDRQLQFLRALLEDNASIDGDVLEISENTWAIHGDI
                     AVDGEVLMAEFATYEEARYVLDQVRGTTLPTSEPESPDRQEGAS"
     gene            5676..6434
                     /locus_tag="VH419_17110"
     CDS             5676..6434
                     /locus_tag="VH419_17110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008357958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY7045394.1"
                     /translation="MKIVVIGGTGLIGSKLVKKLIDQGHDAIPATPDTGVNTLTGAGL
                     ADVLTGAAVVVDVSNSPSLDGAAALEFFETSTRNVLDAEAVAGVGHHVALSVVGTERL
                     GESGYFRAKMAQEKLIKSSSIPYSIVHATQFFEFVKGIADSGTEGNTVRVAPVQIQPM
                     AADDVATALATVTVGAPINGTVEIAGPERFRLDELVGLSLRARHDPHEVVADPDARYF
                     GVELRNDTLVPNDDAQLGATRFEQWLAQQASTGR"
     gene            6437..6592
                     /locus_tag="VH419_17115"
     CDS             6437..6592
                     /locus_tag="VH419_17115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7045395.1"
                     /translation="MSDAYIRVRVPNEPGPDGPTLVYLPDGTALVVDANQLLHLDAGS
                     TRGRDAA"
     gene            6613..>7074
                     /locus_tag="VH419_17120"
     CDS             6613..>7074
                     /locus_tag="VH419_17120"
                     /inference="COORDINATES: protein motif:HMM:NF042641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6582 domain-containing protein"
                     /protein_id="HEY7045396.1"
                     /translation="MARLESAERAKLPDSAFAYVDSRGNRRLPINDAAHVRNALARFN
                     QVAFEDEDARERGRTRLLRAAKKYGIVPVGFITGQLQSERELGRSSTPEPVMLPSGFV
                     TMLLTDIEGSTALVQRLGERYGELLDGVRGLLREAVLASDGHVVETRADEFF"
BASE COUNT         1161 a         2443 c         2366 g         1104 t
ORIGIN      
        1 cctcgtcgac atcgccgaca tcgcgagatc gttcgaactg ccagtgggaa ccacgccgac
       61 ggctgccgat cttctcctcg acgctctggt cgtcttgttc actgagccat tggcttcgtc
      121 gattcctgtc gttcagcggg cactcaccgc cattcgggag gacccagaac tccgaaacac
      181 ccctcgacac ctggcgcgag cctgttacct gccgcaggcg ctcagcgacg acgaatcgca
      241 cttcgcgctg gcgtcggcgt gtgcgacggc atgtcgcgcc caggggtcct tccaagggct
      301 caacgaagca ctggcgtacc tcttcattcg cgagctgcgg gtcggttcga tcgaccgtgc
      361 cgccgacctc gtgctcgaac agcgcgagct ccgagcagtc gtcggccatc cgagcggctt
      421 gaccgacgtc caggacctca tcgtgtcggc gtggcgcgga cgcgaaactg acgtccggac
      481 caaggccccc gatctcgaca ctgtcgcacc cccgtccggc tacctgagtc ggctgatcac
      541 ccacgcgcga atcgttctcg aagttgggct ggggaactac gacgcagccg cgtcgctggt
      601 ccgcgacgac tggtatcaag acttcgcgct cggcgccctg cgcgccgcgg acgccactga
      661 ggcgcaagtc cgcagcggcg acgtcgccgc ggcactgaca tccgccgcct tcctccgcga
      721 acgagcgtca gcgaccgaga gcccgttcga ccgaggactg tggcgacgca gcgaggcctt
      781 gctcgccgac ggcggcgacg ccgagacgtc gtttcgggat tcgatcaccg acctccagac
      841 gtgcggggcc cagctgcacg tggcccgaag tcaactcgtc tacggcgagt ggctccgccg
      901 gcagaagcga cgccgtgatg cccgcgaggt cttggagacg gcacgggaga gcttcgagga
      961 aatgggcgcg caggcattcg cggaacgagc tcgggtcgag ttgctggcca ccggcgcgaa
     1021 tgcccgcagg cgagtcgacg agacccgcca ggtgctcacc ccgcaggagt cgcagatcgc
     1081 ccgcctcgcg ggcggcggct tcacgaaccc cgagatcgct gaacggctgt tcatcagcgc
     1141 caacaccgtc gactatcacc tccggaaggt gtaccggaag ctcgatatcg gctcccgaca
     1201 cgagatcgcc gctgcgttgg atgatcgacg gatcgagcgc gacgacgcgc cggcgaatct
     1261 cacgatcgcc ccgcagatcg tcagctagcg ccctcagtgc ccggccagct cagcgaccac
     1321 gggagcgagt gtttccgcgg cgtcggcgcc gaagacgaaa taggagtagc cgatctcctc
     1381 ccgtcgtcgc tggatttcct cggccgccgc cgccgggtca cccgggagta tggccagcga
     1441 gcccgccgcc cggagcgccg caggatcggt gtcggggggc gccatgaacg gcgcgaccgt
     1501 gtcgcccacg accggcacgt ggagcgcgag ttcgacgtcc cggatgcggc ggaagtcacg
     1561 tgcccgctgc gcgacttcgc tgggggggtc atccggcaac acggcgaagg tgactgtatc
     1621 tgcgatctcg gccgccagtg cctgggcctt cggtccacgc acggccatca ccacaggcgt
     1681 gtggaggtcg gggccgtcga gatggcgcag cgccgccacg gtctcacgca cctgagccag
     1741 ccgctcgccc gatggcacca cgggcagtcc caactcgcgg agctcatcct cgatccctgg
     1801 ccggccggtg ccgatgccca tctcgaagcg accgtcagtg agcaccgaca gcgagtgtgc
     1861 ttcccacgcg gtgctccacg ccgggtggag cggagaggca tacacccagg tgcccacccg
     1921 caggtcggcc agagccgccg cgaacgccag ggtggggccg ggcgccggct gccattgcgg
     1981 gacgtctggc atcagcagcg tcgagaagcc gctgtcggcg atgcggcgca cccggtctct
     2041 ccaggtcgcc gggtcaggtg tgaccggtgc aacgaccccg aatcggaacg gtctggtcat
     2101 gccttgaccc ctctcgcagg agcgatgccg cgggccgacg gcggcgtctc gtcgaactcg
     2161 tcgtggcggc ggtaccagaa cggcagcggc gcctgcggcc agccttcagg cacgtcttcg
     2221 tcctcgccct gcccgccata gggtgtgagg gagaacaggc tccagacgct gccgatcttc
     2281 tggaccatcg gaccgtgctg gaggaagtaa gtgcggtaga cgtcgtcgcc gtcgcgcagg
     2341 aagacgttga ggccgaacca ctcctcgacc ccgaagtcgc tcgagaagcg ctcggtgcag
     2401 atcgtgtacc acggcacgtc ggtccagccc atgcgctcgg cgtagcggcg caggttggcc
     2461 tggggtgccg gtgaggccat ggcgaaggtg atgtcgcggg cgtgcaggag gccgagctcc
     2521 gggatgccgt cggcgaacga cgagcagccc acgcagcccg cgtccggcca gccaccgacg
     2581 ccttcctcga agaagaaccg gtacaggatg agctggctgc gcccgtcgaa caactcgggc
     2641 agcgaccgtt cgccgtcggg gccctcgaag cggtactggt gctcgacgcg caccatcggc
     2701 atgcgcttgc gggcggcggc gagctcgttc atcgccgtgg cgacggcctt ttcgcgttcg
     2761 gtcatggtgg ccagggcggc gtcccattcg gcctgggtga cgacaggcgg gcgagcgacc
     2821 gtcatggctg tgccttcgtc gtgtccgggg gcccggccag caccgtgggg acgtactcga
     2881 gcagctggct gcgaccgtcg aaggtgcgac tgctgatcat gtcgagggcg acgtcggggt
     2941 agccgtcgta gatccggtcg cgaccggtgc tgccggtgat gacgggaaag acgaccacgc
     3001 gaaagcggtc cacgaggcca gcgttgagga gcgagcggca cagggtgagg ctgccgatgg
     3061 tgcgcatcga cgtggaacct tcgtccttca tcttccgcac ggcgtcaacc gcgtctcggt
     3121 ccacgagttg cgtgttgggc cacgacagcg gctccttcaa tgtgctcgag aagacgacct
     3181 tcaacatgcc cccgagcgca tcggtaccgg gctcacccga ggctgcgaag ccggacatga
     3241 tccggtacgt cgtcgcgccc atcaggatcg tgtactcgcc ttctggctgt ccccccaacc
     3301 agccgaggta ctccggacct tccagccccc accagccccg ccagccttcg gcggcgccgt
     3361 agccatccag cgatgtgatg aagtcgacga ggagttctgg catcaccgtc tcctttcggc
     3421 gcgggtcgcg cgacatcggt ctgttcacac gattccctgt ttgaccggtc atcaacgcgt
     3481 gacgtccacc cgtacgggtc tttgaaaccg cgtcggtcgt ggatccggtc aggcccgacg
     3541 cagtcggtcg aggacccgat cgtcgacgca ctcctcgggg atggtctccg gcccggtgaa
     3601 acagccgatg agaaccggga agaactcggg atccatggcg gccatcttct cggccatcag
     3661 ctggcggcga gccatgaggt tgtcgcagtc ctcggcctgg accaccgaca tgatgtccgc
     3721 cgccagccgc agtcgccgca tgcggtcgac ccgttccgtc gcgtagggcg caaagtcggc
     3781 cggctgtcgg gctccatcga ccacgagatc gcggaccatt cgagcgtcgc gcatcgcgat
     3841 cgagagacct tgcccgatga tcggatcgtt gtgcccggcc gcgtcgccga tcaggacgac
     3901 accaggggca gccggcgcgt ccgtccacgt gtcgtcgccc ggataggtgg cgcaggggcc
     3961 agccggcgtc gccctggcca cgcgctcggt ctcggggtag cacgacgggt tccaggccgc
     4021 catgaacttg gccacgccat cctggccggc aaagcgatgc tgcatcgacc ggccgccgca
     4081 caggtacagg cgcgcccgcc cgccgccctg gtgaaacatc agggagaagt agtcgccctc
     4141 gctcacgagc acgtcgtagt cgtccggaac accgtcgagc ccgtcgacca gcagccccgc
     4201 gatgtagttg atcggttcct ggcggtggag ctcgatcccg gcctgccggc ggatcgtcga
     4261 cgcccggcca tcggcaccca cgacaagtgt cgtcttgacg gtcgcggcgt cgccgtcgga
     4321 acggtacgag acagccggtt cgggccccgc cgtgagggtc acgtccgcca cgccgcgcac
     4381 caccgtcgcg ccggccccgg ccgccgcgtc caaaagtgcc tgacaggcgt cggggtgacg
     4441 caggttcaac gtgccgggga tgtccggaag cagcatgctg gccgggatat cgccgggttc
     4501 gccgattcct tcgacgtatt gcctccacac gggagcgatg tgggcgcccg ccttcagcag
     4561 cacgtcctcc acacccaggc ggcgggcctc ggcgagaccc cacagctgca tgctctcgcc
     4621 ccggacgcgg tcggggaact ccttgctcgc ctcgagcaca acgacgccga ggccgctctg
     4681 ggcgagtccg tacgccagcg cagcaccgcc gatgccaccc cctactacga cgacgtcgca
     4741 cgcggtcatg gccggcgagt atcgcacgcc accaagcggt ggcggtctag aagctgcggt
     4801 ccgtgacgac gttcaggttg atgaactgcg gctcgccctt ggacatcgcc atcaccttct
     4861 gcgcgacctg atcggtgccc ggcagggccg agttccgggc cgccgcctcg gcgtcgggga
     4921 agatggcgat gagcacgcgc aggctcgggt catcggctcc gacgcagccg atcgcgcgct
     4981 ggatggtgcg ttctccttcg gtcagcttca cgaactcgtc ttcgagcgcc gtgaactcct
     5041 cgatcgagtg gtcactgtga gtgatattga cctggatgaa caccgcgaca acctccctca
     5101 cgtcgatggg gcccccaccg tagaccggat cgccggcccg gcccgctcgg gcaatgacct
     5161 ggcccgacgg gatccacact ggggactttc acggatgcga aggaccgccc gtcagggaga
     5221 cgctcggtgg tgacagttcc cgatcggacc tcgagcagga aggacgaacg atgtcaggtc
     5281 agtcccccac cgtcgtgctg gtccgcggcg gcttcgccgc cgccatggcc gcaacgatcc
     5341 tcgacgctct agcctcggtg tgacggaccg atgaactcgg tacagcaatg ctgtctcgtg
     5401 agtgaggttg tcatggaccg cccgcgcgat ccagacctgg accgccagct tcagttcctg
     5461 cgagcgctcc tggaggacaa cgcgtcgatc gacggcgacg tgctcgagat cagcgagaac
     5521 acctgggcga tccacggaga catcgccgtt gacggcgagg tcctgatggc cgagttcgcc
     5581 acctacgagg aagcgaggta cgtgctcgac caagtccgcg gcacgacgct cccgacgagc
     5641 gaacccgaat cgcccgaccg acaggaaggt gcatcgtgaa gatcgtcgtc atcggcggca
     5701 ccgggctgat cggctcgaag ctcgtcaaga agctcatcga ccagggccac gacgccatcc
     5761 cggcgacacc cgacaccggg gtgaacacgc tcactggtgc cggcctggcc gatgttctca
     5821 ccggtgccgc ggtcgtggtc gacgtgtcga actcgccctc cttggacggc gccgcggcgc
     5881 tcgaattctt cgagacgtcg acccggaacg tgctcgatgc agaggcggtc gcgggcgttg
     5941 gccatcatgt ggcgctctcg gtcgtcggca ccgaacgctt gggcgaaagc ggctacttcc
     6001 gcgccaagat ggcgcaggag aagctcatca agagctcgtc gatcccctac tcgatcgttc
     6061 acgccacgca gttcttcgag ttcgtcaagg gcatcgccga ctccgggacc gagggcaaca
     6121 cggtgcgagt cgcaccggtg cagatccagc cgatggccgc cgacgacgtc gccactgcgc
     6181 tcgccacggt cacggtcggc gctccgatca acggcaccgt cgagatcgcc ggacccgagc
     6241 ggttccggct cgacgaactg gtcggactca gcttgcgcgc ccgccacgat ccccatgaag
     6301 tcgtcgccga cccggacgcc aggtatttcg gcgtcgaact gcgcaatgac accctcgttc
     6361 cgaacgacga cgcccagctc ggcgcgaccc ggttcgagca atggctcgcc caacaagcgt
     6421 caaccgggag gtgaccgtgt ccgacgcata catccgagtc cgggtcccga acgagcccgg
     6481 gcccgacgga cccacgctgg tctacctgcc ggatggaacc gcgctcgtcg tggacgccaa
     6541 ccagttgctg cacctcgacg cgggctcgac tcgcgggcgg gacgccgcct aggcccttgt
     6601 taggttcgcg tcatggcgcg actcgagtcg gctgagcgag ccaagctgcc ggacagcgcc
     6661 ttcgcctacg tcgactcgcg tggcaaccgc cggttgccga tcaacgatgc ggcccacgtc
     6721 cgcaatgccc tggcccgatt caaccaggtc gcgttcgagg acgaggatgc ccgcgaacga
     6781 ggccgcacgc ggttgctgag ggcggccaag aagtacggga tcgtgccggt cgggttcatc
     6841 accggacagt tgcagtcgga gcgggagctg ggccggagtt ccacgccgga gccggtgatg
     6901 ctgccgtccg gcttcgtgac catgctgctg accgacatcg agggctcgac ggccttggtc
     6961 cagcggctcg gtgagcgcta cggggagctg ctcgacggcg tccggggcct gctccgggaa
     7021 gcggtgctgg cctcggacgg tcacgtcgtc gagacacgag ccgacgagtt cttc
//
LOCUS       DATBFZ010000563        10056 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_6233911, whole genome shotgun sequence.
ACCESSION   DATBFZ010000563 DATBFZ010000000
VERSION     DATBFZ010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 10056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10056
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6233911"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..593)
                     /locus_tag="VH419_17125"
     CDS             complement(<1..593)
                     /locus_tag="VH419_17125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7045397.1"
                     /translation="MSEQRGGADNERYDRDLLLYGSKRDQVLDLWEVKRYGSDTFGDP
                     DAISVYGMTPDDWFARGIRLLGRTVVECTRDVLAREIAADVATVAQTSSSVPLVLDPF
                     VGSANTLYWIQQRLPTSPAIGVDLDGAICELTKRNLDVVQSRIDLLNLDYAAGLDRVA
                     VQTSQPIVVYVAPPWGHALDPEVGLDLSRSEPPVAHVV"
     gene            773..2230
                     /locus_tag="VH419_17130"
     CDS             773..2230
                     /locus_tag="VH419_17130"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEY7045398.1"
                     /translation="MTDVAGEQPTRGLRRGVVGFNGVLFQSITFMAPAIATAFSIPAG
                     IAYSGGGAPLSVIFALVASMLAAISIGQLSKHMPTAGSFYTYVSNGLHPIVGFIVAWG
                     FLLGILVGGPFLALQMGFIVAGTTNSEWGWSPDLWWVWTVLVCLLVFWLGYRGIRAST
                     ETGILLGAFEILVFVALSLTLIVQAGGDNTLEVFGTHYANNPDYSGFSGVIVGSIYTI
                     LAFIGFEEAAPIAEEAHEPHRNVNLAIIFSCLGIGIFYLLNTYASTITFGPEQMTGFV
                     GAGDGNPWQNLLARDAWGTIGFVLVFIALINSVIANQNAANNSSTRTMFSMGRIRLLP
                     TQYGLLSRGSPMLALITQLVVSVGVALWLGNQYTPYDGFVILATILVDIFAPMYILLN
                     IACIAYFWRYRRAEFSWWLHGLIPLLGALAFIPAFFAGAGIPAFSFINPLPHPYSYAG
                     PAAAIWMGIGIVYLIVLMVTRPGQITETKRVFAEE"
     gene            2235..3245
                     /locus_tag="VH419_17135"
     CDS             2235..3245
                     /locus_tag="VH419_17135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020389174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HEY7045399.1"
                     /translation="MDVLNYRPNPDELAWTLGGVKPVRRVKPGTVLEVWTEDAFGGKV
                     RGTDDLVSKVIEFPFVNPLTGPFYIEGAEPGDTLAVHFVSIEPSREWAASSTIPFFGA
                     LTSTHVTAMLQEPLPERVWMYDVDRAARTVKVRASQGGAEVVMPLDPMHGTVGVAPAN
                     LEVRSSLVPDMHGGNMDTPEMRAGVTCYLGVNVEGALFALGDGHDRQGEGETCGVAIE
                     AAMDTVVIVDLLKGVSTPWPRLESDDYLMSAGSARPLEDAFRIAQHDLITWIAVECGL
                     DPLDGYQLVTQASESPIANVCDPNYTVIAKIRKAYLPSHDAYAGMHTRLREISAAYKS
                     ER"
     gene            3403..3705
                     /locus_tag="VH419_17140"
     CDS             3403..3705
                     /locus_tag="VH419_17140"
                     /inference="COORDINATES: protein motif:HMM:NF016141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEY7045400.1"
                     /translation="MDVGSFIAAILLGFVAGAIGRALVPNDAFRDMAGWKSWATSTVL
                     GLIGALLGYWIFTGLLGIGDTDKFDWGGIIGAIIGAVIVVAIASWGFKRYARGRTA"
     gene            complement(3728..4081)
                     /locus_tag="VH419_17145"
     CDS             complement(3728..4081)
                     /locus_tag="VH419_17145"
                     /inference="COORDINATES: protein motif:HMM:NF016326.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH938/NDUFAF3 family protein"
                     /protein_id="HEY7045401.1"
                     /translation="MDVRLASFGAIEAEGQRYEHDIVVDHGVVRKRSKGPSKAYRGRF
                     GHTPLSVDETLPWDTDRLIIGTGAYGSLPVMAEVSAEAEHRGIELITVPTEEACRLIS
                     SLDAADVCAVLHVTC"
     gene            complement(4110..4739)
                     /locus_tag="VH419_17150"
     CDS             complement(4110..4739)
                     /locus_tag="VH419_17150"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY7045402.1"
                     /translation="MIAQPVLQTDRLLLVPLAERHLDLEIELDSDTEVLRYIWGRART
                     PDEVAEDHAERMALGRKFPGLGYWAAFGTDGGARDSHRPSTEEAGHFVGLMMLPPAHR
                     RGQPEDPGVASLGYRIVRRCWRQGLATEASRTLLRHAFDTVGVRRVVAQTMAVNAGSR
                     GVMSALGMSHVRTVFHDWDEPVRGSELGDVEYELTLAEWRRRRSTTSWA"
     gene            complement(4770..5249)
                     /locus_tag="VH419_17155"
     CDS             complement(4770..5249)
                     /locus_tag="VH419_17155"
                     /inference="COORDINATES: protein motif:HMM:NF018663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY7045403.1"
                     /translation="MAKVYPCLWFDGNAEEAANFYVTLLPDSHVDKLWHSPAETPSGP
                     AGMVLTVDFTLAGQQFQGLNGGPDFTFNEAVSFVIDCEDQAEVDRLWDALTADGGEPG
                     PCGWLKDRYGLGWQIVPRRLDELLNDPDPERARRAMEAMLEMGKIEVAGLERAADAA"
     gene            complement(5515..5587)
                     /locus_tag="VH419_17160"
     tRNA            complement(5515..5587)
                     /locus_tag="VH419_17160"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5552..5554),aa:Lys,seq:ttt)
     gene            5710..6213
                     /locus_tag="VH419_17165"
     CDS             5710..6213
                     /locus_tag="VH419_17165"
                     /inference="COORDINATES: protein motif:HMM:NF018590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HEY7045404.1"
                     /translation="MADNDGVFLYIATYPDEAAARADYDVVKALHELDAVGRYDAAVV
                     TKDDKGKVHVNKDETSTRRGAWGGIAVGALVGILFPPSILGSAAVAGVVGGFAGHLWG
                     GLSRSDVKELGELIDEGQAALLVIGDLTVSKALEKAQLHAIKEVRKQVNVDLDELEKD
                     IKDADKA"
     gene            6834..7412
                     /locus_tag="VH419_17170"
     CDS             6834..7412
                     /locus_tag="VH419_17170"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEY7045405.1"
                     /translation="MTDTASNEPHPPDTATNPASAARRTRRVTTRDIDLFTEITGDRN
                     PVHYDAELAARSRFGGIVVQGGVTSGLLNALVAEDLPGPGSVFLNVNWNFRAPVKPGD
                     LLTAEARVTKEHPDKPVTWLDTTIINQHDEVVLDGSAVVWRDPVVAAATAARGTPEDI
                     SPMRPARVSPSHYLHAHHIPAPARPATTRRKS"
     gene            7409..7999
                     /locus_tag="VH419_17175"
     CDS             7409..7999
                     /locus_tag="VH419_17175"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEY7045406.1"
                     /translation="MTVTTESPRRNGVDVATLFATLDAVKGQPEIAKFQFRATNTWQS
                     GTHSRSEISGFHGALQEMQHKQVTVVDADHPEVLVGTDKAPTPVEYLLHAIAACLTAG
                     IGNIASARGVDLTSVSSTVEGDIDLLGILGLSDGSVRNGYEQMRVTFHIEGDADEDTL
                     RGIVEQSRRRSAVYDVLTNPTPVSVEVVATRRPGDR"
     gene            8071..9330
                     /locus_tag="VH419_17180"
     CDS             8071..9330
                     /locus_tag="VH419_17180"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF025114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY7045407.1"
                     /translation="MTTRIDTVVIGAGHAGLAVSHLLSRAGRDHVVLDRGRIAERWRS
                     ERWDSLHLLTPRWMTRLPGWQYSGAGLEGFMPKDEFVRHLERYASESPLALDQDVLAV
                     DAAGDGYHVVTDRAAWQTSNVVIATGPGRPYLPPTVERLGADVRVVTTSRYRNPEQLT
                     AGGVLVVGASASGVQIADELARSGRRVVLSTGNHNRMPRRYRGMDIFWWLEQTRWLER
                     RSNDAHDAKATHPEPSLQLAGRDSKDPRGADVDLRSLHHAGVELVGRLDHVDRHRVTF
                     RTDLATTTQAADARMHRFLDMVDTYVELNGLSSEVEPPDRPQPFRTRRGHSRLNLRSE
                     GISTVILATGFRPHHPWLRVPVLDERGAIRQSRGVTAAPGLYVVGQRYQRHRGAPFID
                     SARHSAHDVVTHLCTGDLAAACVPEPS"
     gene            9327..>10056
                     /locus_tag="VH419_17185"
     CDS             9327..>10056
                     /locus_tag="VH419_17185"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEY7045408.1"
                     /translation="MTSAGETTYDVVVVGARVAGASTAMLLARAGAHVALVDRAVYGS
                     DTVSTHGLMRAGVLQLHRWGLLDRIVTAGTPPVRRTTFHYDGTRGETNVQISIRPSGG
                     IDALYAPRRHVIDRVLVDAAAEAGVDVLHETRVDDVVFADDGRVCGVAVHTPDGRRVL
                     RSRYVVGADGIGSLVARAVRAETVLEGRGASAVRYAYYSGLEPTGYEWAYADGAAAGI
                     IPTNDDVSCVFVSTTPLRMRTLRTN"
BASE COUNT         1681 a         3426 c         3300 g         1649 t
ORIGIN      
        1 acgacgtgag ccaccggcgg ctcggagcga ctcagatcaa gacccacctc tggatcgagc
       61 gcatgacccc agggcggcgc gacgtacacg acgatcggct ggctcgtttg taccgcgaca
      121 cggtcgaggc cggctgcgta gtcgaggttc aggaggtcga tgcgtgactg gacaacatcg
      181 aggtttcgct tcgtgagctc acagatggcc ccatccaggt cgacgccgat cgccggtgac
      241 gtcggcagtc gctgctgaat ccagtagagc gtgttggccg atccgacgaa cgggtccaac
      301 acgagcggca ccgatgacga ggtctgcgca acggttgcca cgtcggcggc gatctctcga
      361 gccagtacgt cgcgcgtgca ctcgaccaca gtccggccaa gcagccggat gccgcgtgca
      421 aaccagtcgt ccggcgtcat gccgtacacg gagatggcgt ccgggtcacc gaacgtgtcg
      481 ctgccgtagc gcttgacctc ccacaggtcc aggacctgat cgcgcttcga gccgtagagc
      541 aacaggtcgc ggtcgtatcg ttcgttgtcg gcgccaccgc gctgttcgct cacccgtcca
      601 tggtgggcca tccggtcgat cagcggcctg gcgacgtgct ggactcgcga tctcgatcgt
      661 gttgcggtcg agcagcggac aaaggccggc cgcgtccatc gacgtaacac gcggcacggc
      721 gctagagtcg ttcggcggaa aactcggggc cacttctcaa caggagggcg cgatgaccga
      781 cgtcgctggt gaacaaccga cccggggttt gcggaggggc gtcgtcgggt tcaacggggt
      841 cctgttccag tccatcacgt tcatggcgcc cgcgatcgcc accgccttct cgatcccggc
      901 gggcatcgca tacagcggcg gcggcgcgcc gctctcagtg atcttcgcgc tcgtcgcgtc
      961 gatgctcgcc gccatctcga tcggtcagct ctccaagcac atgccgaccg ccgggtcgtt
     1021 ctacacctac gtcagcaacg ggctccaccc catcgtgggc ttcatcgtcg catgggggtt
     1081 cctgctcggg atcctcgtcg gcgggccgtt cctcgcgctg cagatgggct tcatcgtcgc
     1141 cgggaccacg aactcggagt ggggatggtc gccggacctg tggtgggtct ggacggtgct
     1201 ggtctgcctc ctggtcttct ggttgggcta ccgcggcatc agggcgtcga ccgagaccgg
     1261 catcctgctc ggtgcgttcg agatcctcgt cttcgtcgcg ctctccttga cgctgatcgt
     1321 ccaggcgggt ggtgacaaca cgctcgaagt gttcggcacc cactacgcca acaacccgga
     1381 ctactccggc ttctccggcg tgatcgtcgg gtcgatctac acgattctcg cgttcatcgg
     1441 cttcgaggag gcggcgccca ttgccgaaga ggcccacgag ccgcaccgga acgtgaacct
     1501 cgccatcatc ttctcctgcc tcggcatcgg gatcttctac ctcctcaaca cctacgccag
     1561 cacgatcacg ttcggaccgg agcagatgac cggcttcgtc ggtgccggcg acggcaaccc
     1621 gtggcagaac ctgctggccc gtgacgcctg gggcacgatc ggcttcgtcc tcgtcttcat
     1681 cgcgctcatc aactcggtga tcgcgaacca gaacgctgcc aacaactcat ccacgcgcac
     1741 gatgttctcg atgggccgga tccggttgct gccgacgcag tacggcctgc tgagccgcgg
     1801 ttcgccgatg ctcgcgctga tcacgcagct cgtggtgagc gtcggcgtag cgctctggct
     1861 cgggaaccag tacacgccgt acgacggctt cgtgatcctg gcgacgatcc tggtcgacat
     1921 cttcgcgccg atgtacatct tgctgaacat cgcctgcatc gcctacttct ggcgctatcg
     1981 ccgggcggag ttcagctggt ggctgcatgg gctgattccg ctcctcgggg cgctcgcgtt
     2041 catcccggcg ttcttcgccg gtgcaggcat ccccgccttc agcttcatca acccgctgcc
     2101 gcacccgtac tcgtacgccg gacctgctgc cgcgatctgg atggggatcg gcatcgtgta
     2161 cctgatcgtc ctcatggtga cccggccggg acagatcacc gagaccaagc gggtgttcgc
     2221 agaggagtga gaccgtggac gtcctcaact accgtccgaa tcccgatgag ctcgcctgga
     2281 ccctcggtgg ggtgaagccg gttcgccggg tcaagcccgg cacggtgctc gaggtgtgga
     2341 ccgaggacgc cttcggcggg aaggttcgcg ggacggacga cctcgtgtcg aaggtgatcg
     2401 agttcccgtt cgtcaacccg ctgaccggac cgttctacat cgagggcgcg gagcctggcg
     2461 acacgctggc cgtgcacttc gtctcgatcg agccgtcccg cgagtgggcc gcgtcctcga
     2521 cgatcccgtt cttcggagcg ctcacgtcga cccacgtcac cgcaatgctt caggaaccgc
     2581 ttcccgaacg ggtatggatg tacgacgtcg atcgtgccgc gcggaccgtg aaggtgaggg
     2641 ccagccaggg tggtgccgaa gtggtcatgc cgcttgaccc gatgcacgga acggtcgggg
     2701 tggcaccggc caacctcgag gtgcgctcct cgctggtgcc cgacatgcac ggcgggaaca
     2761 tggacacccc cgagatgcgc gctggtgtca cgtgctacct cggcgtcaac gtcgaagggg
     2821 cactgttcgc ccttggtgac ggacacgacc ggcagggcga gggcgagacc tgcggtgtcg
     2881 cgatcgaagc ggcgatggac acggtcgtca tcgtcgacct gctgaaaggc gtgtccacgc
     2941 cgtggcctcg gctcgagagt gacgactacc tgatgagcgc cgggtctgcg cggccgctcg
     3001 aagacgcgtt ccgcatcgcc cagcacgacc tcatcacctg gatcgcggtc gagtgcggtc
     3061 tggatccgct ggacggctac cagctcgtca cgcaggcgtc cgagagcccg atcgccaacg
     3121 tgtgcgaccc gaactacacg gtcatcgcga agatcaggaa ggcctacctc cccagccacg
     3181 acgcgtacgc cggcatgcat acccggctgc gcgagatcag tgcggcgtac aagtccgagc
     3241 gctgacacgc catgagggcc cgcgccgtcc acgatcttcc cgccaatcgc cagcaccgcg
     3301 gcgcgcggta cggggaatgt cggttggcgc gtactaacct cggagttgtg cacattcgtg
     3361 cacgagaacg gagcgaattg ggctctccga cggggaggca tcatggacgt cggcagtttc
     3421 atcgcggcaa tcctgctcgg tttcgtggcc ggcgccatcg gtcgcgctct cgtaccgaac
     3481 gacgcgttcc gagacatggc tggatggaag tcgtgggcga cctcgacggt gctcggtctg
     3541 atcggggcct tgctcggcta ttggatcttc accggcctgc tggggatcgg tgacacggac
     3601 aagttcgact ggggcgggat catcggcgcc atcatcggtg cggtcatcgt cgtggcgatc
     3661 gcgtcgtggg gattcaagcg ctacgcccgt ggccgcaccg cctgacgttc gggcctgggc
     3721 tgcgctgtca acaggtgacg tgcaagacgg cgcagacgtc ggctgcgtcg aggctgctga
     3781 tcagccggca agcttcctcg gtcgggacgg tgatgagctc gatgccacgg tgttccgcct
     3841 ctgccgagac ctcggccatg accggaagcg agccgtacgc ccccgtgccg atgatcaacc
     3901 tgtcggtatc ccagggcagg gtctcgtcga ctgagagcgg cgtgtgaccg aagcgccctc
     3961 ggtacgcctt ggagggtccc tttgagcgtt tccgcacgac tccatggtcg accacgatgt
     4021 cgtgctcata ccgctgccct tcggcctcga tcgcaccgaa cgatgccagc cgcacatcca
     4081 tgtcagattc tctcctcctc ggtccgtgct cacgcccatg acgtcgtact ccgccggcgc
     4141 ctccactcgg ccagggtcag ctcgtactcg acgtctccca gctcggagcc gcggactggc
     4201 tcgtcccagt catgaaacac ggtgcgaacg tgcgacatcc cgagcgcgga catcacgccg
     4261 cgagagccgg cgttgaccgc catcgtctgg gcgacgacac ggcgtacgcc gaccgtgtcg
     4321 aacgcatgcc gaaggagcgt gcgtgacgcc tccgtcgcga gtccctgccg ccagcaccgg
     4381 cgcacgatcc gatagccgag actcgcgacg ccggggtcct cgggctgccc gcggcggtgc
     4441 gccggcggga gcatcatgag ccccacgaaa tgcccggcct cctccgtcga gggacggtga
     4501 gagtcacgcg cccctccgtc ggtcccgaac gcggcccagt agcccagacc aggaaacttg
     4561 cgtccgagcg ccatccgctc cgcgtgatct tcggccacct cgtcaggtgt gcgggcgcgt
     4621 ccccagatgt atcgcagcac ttcggtgtcg gagtcgagct cgatctcgag gtcgaggtgg
     4681 cgttcggcga gcggaacgag caggagccgg tctgtttgca ggacgggctg cgcgatcaca
     4741 cgtcaatcat ggcccgcact tcgtgtgagt cacgccgcgt cggcagcccg ctccaacccg
     4801 gcgacctcga tcttgcccat ctcgagcatc gcctccatcg cgcggcgggc cctctcggga
     4861 tcgggatcgt tgagcagctc gtcaagcctg cgcggcacga tctgccagcc caacccgtac
     4921 cggtccttga gccagccaca cggaccgggc tcgccgccgt cggccgtcag cgcatcccag
     4981 agccgatcga cctcggcctg gtcctcgcag tcgatgacga acgacaccgc ttcgttgaac
     5041 gtgaagtccg gaccgccgtt gagcccctgg aactgctggc cggcgagcgt gaagtcaacg
     5101 gtcagcacca tccccgccgg ccccgacggc gtctcagccg gcgagtgcca gagtttgtcg
     5161 acgtggctgt ccggcagcaa cgtcacgtag aagttggccg cctcctcggc gttgccgtcg
     5221 aaccacagac aggggtacac cttggccatc gcgtcctcct tcgatcagga cccgctcggc
     5281 gatgtggaga gcctccgggt cacctcaaca cgtccttgaa cgtaagaccg ggccgcagac
     5341 tgatactcat cggtcccgac gagctgcttc ccttggcgga cggcgggggc ggggacgagg
     5401 cctccgtcga gcacgcccgt tgatacccgc gtcgggaacc aaccaaagct gcgctgcaag
     5461 cgtgtgagcc ttccgcgagc acagagcgcc ctcatccgac gtggggaacg tgcgtgggcc
     5521 ccgtgggact cgaacccaca acccgcggat taaaagtccg ctgctctgcc aattgagcta
     5581 gaggccccag ccggcgtacg gaaggacgca ggcaacaagg atagggaccg aactccggag
     5641 ggccactgtc cgactcggac ctccatctgc cagcatcggt cggaggaccc agaggaagga
     5701 acgtcgacca tggcggacaa cgacggcgtg ttcttgtaca tcgcaaccta tcccgacgag
     5761 gcggcggccc gcgcggacta cgacgtggtg aaggcgctgc acgaactcga tgcggtgggg
     5821 aggtacgacg cagcggtcgt caccaaggac gacaagggca aggtccacgt caacaaggac
     5881 gagaccagca ctcggcgtgg ggcgtggggc ggcatcgccg tgggtgcctt ggtcggcatc
     5941 ctgttccctc cgtcgatact cggctcggct gcggtggcgg gcgtggtcgg aggcttcgcc
     6001 ggacacctgt ggggcggctt gtcccgcagc gacgtgaagg agctcggcga gctgatcgac
     6061 gaagggcagg ccgccctgct ggtgatcggt gacctgaccg tgagcaaggc gttggagaag
     6121 gcgcagctgc atgcgatcaa ggaggtccgc aagcaggtca acgtcgatct cgacgagctc
     6181 gagaaggaca tcaaggacgc tgacaaggca tagcgactcc cctcggcccg cgaaccggcc
     6241 cttccggaaa gccacctacg ccaccattcg gacaggtgtc ggtggtggtt tgcctcggta
     6301 gccatgcaca cggacgggta gacaggcaga tctgccttgg gaggggtggg tttgcctggt
     6361 tagccatgca aaccgccgtt cgtcactgcc gatcctttca gcccatgacg aaggaccgcc
     6421 cgggcgatca cggccacttc ctcgcggcca tgtccgcccg aaaaccctgt ggagaaccgg
     6481 agtcacctac cgggtcacgc ggaatgcggg aaaggtcagt tcaatacgcc ggtggaaacc
     6541 tgcactttcc cgcaatccgc ggaagcgggc tgcgagcgtt tccagcgacg aaggcgggtc
     6601 gatgtcggct cgtgaagccg tcgtcgtgcc tgtcgtgcct gtcgtacgtg ggcatcgtcg
     6661 tactcctggg tgacgcgcaa cgccacgtaa tggacaaacg ggttcatggt gtcctcctga
     6721 tcggtgatgg cgtccagcgt tgcggcccga ccttcgcctg accttccccg gcagaatccg
     6781 cgtcccacga cgagggaagg ctgtgggaag ggtgctccga caaggtgtgg ggcatgacag
     6841 acaccgcatc caacgagcca cacccaccgg acaccgccac caacccggca agtgctgccc
     6901 ggcgtacgcg cagggtgacc acgcgggaca tcgacctgtt caccgagatc accggtgacc
     6961 gcaacccggt ccactacgac gccgagctgg cagcacgcag caggttcggg ggcatcgtcg
     7021 tccagggcgg cgtgaccagc ggcctgctga acgctttggt ggccgaggac ctgcccggcc
     7081 cggggagtgt gttcctcaac gtcaactgga acttccgcgc gccggtcaag cccggagatc
     7141 tgctcacggc tgaggcacgc gtgaccaagg agcatcctga caagccggtc acctggctcg
     7201 acaccacgat catcaaccag cacgacgagg tcgtcctcga cggaagcgcc gtcgtgtggc
     7261 gggaccccgt ggtcgcggcc gccacggcgg cccgaggcac cccagaagac atctcaccga
     7321 tgagaccggc gcgggtcagc ccgagccact acttgcacgc acaccacatc ccagcgccgg
     7381 cgaggccggc caccaccagg aggaagtcat gaccgtcacc accgagagcc cgcgccgcaa
     7441 cggcgtcgac gtcgccacac tgttcgcgac cctcgacgcc gtcaagggac agcccgagat
     7501 cgcgaagttc cagttccgcg ccaccaacac ctggcagagc ggcacccaca gccgttcgga
     7561 gatctccggc ttccacggcg ccctgcagga gatgcagcac aagcaggtca ccgtggtcga
     7621 cgcggaccac cccgaagtgc tggtcggcac cgacaaggcg cccactcccg tggagtacct
     7681 cctccacgcg atcgcggcct gcctcaccgc cggaatcggc aacatcgctt ccgcccgcgg
     7741 tgtcgacctg accagcgtca gctccacggt cgagggggac atcgacctgc tcggcatcct
     7801 cggcctctcg gacggctcgg tccgcaacgg ctacgagcag atgcgggtca cgttccacat
     7861 cgagggggac gccgacgagg acacgctgcg cggcatcgtc gagcagtcgc gtcgtcgctc
     7921 cgccgtgtac gacgtgctca ccaaccccac accggtctcc gtcgaggtcg ttgccacgag
     7981 acggcccgga gaccgctgaa ccaccactca ctcagccacg cggccccggc cggcaggcgc
     8041 tgccggccgg gccgcaccag aaggggaatc atgaccaccc gaatcgacac cgtcgtcatc
     8101 ggcgccggtc acgcgggcct cgcagtcagc catctgctct cccgcgccgg gcgtgatcac
     8161 gtggtgctcg accgcggccg gatcgccgag cggtggcggt ccgaacgctg ggactcgctg
     8221 cacctgctca cgccgcgctg gatgactcgg cttcccggat ggcagtactc cggcgccggc
     8281 ctggaaggct tcatgccgaa ggacgagttc gtccggcacc tcgagcggta tgcatcggag
     8341 tcgccgcttg ccctcgacca ggacgtgctc gcagtcgatg ccgcaggaga tggataccac
     8401 gtcgtcactg atcgagcggc gtggcagacg agcaacgtcg tcatcgccac cggcccgggt
     8461 cgcccatacc tcccgccgac cgtcgaacgg ctcggcgccg acgtccgtgt ggtcacgaca
     8521 tcgagatacc gcaaccccga acagctgact gctggtggtg tgctcgtcgt gggcgcctcg
     8581 gcgtccggcg tgcagatagc cgacgagctc gcccgttccg gccgacgagt tgtcttgtcc
     8641 accgggaacc acaaccggat gccccgccgc taccgcggca tggacatctt ctggtggctc
     8701 gagcagacac gttggctgga acgtcgaagc aacgatgccc acgatgcgaa ggcgacgcat
     8761 cccgagccat cgcttcagct cgcaggtcgc gacagcaagg acccgcgcgg cgcggatgtc
     8821 gacctgcggt cgctccacca cgccggcgtc gagctggtgg gccgcctgga tcacgtcgac
     8881 cggcaccgcg tcacgttcag gaccgacctg gcaacgacga cgcaggcagc cgacgcgcgg
     8941 atgcaccgct tcctcgacat ggtcgacacc tatgtcgagc tgaacgggct cagctccgag
     9001 gtcgagccgc ccgaccggcc acaaccgttc cgcacccgcc gtgggcacag ccggctgaac
     9061 ctgcgatccg aggggatcag caccgtgatc ctggccaccg ggttcaggcc acatcatccg
     9121 tggctgcgcg tgcccgtgct cgacgagcgc ggcgcgatcc ggcagtcgcg tggtgtgaca
     9181 gccgcccccg ggctgtacgt cgtcggacag cgctaccagc gccatcgcgg tgcaccgttc
     9241 atcgactcgg cccggcacag cgcgcacgac gtggtgacac atctgtgcac cggtgacctg
     9301 gccgcagcct gtgttccgga gccgtcatga cgtcggcggg tgaaacgacg tacgacgtgg
     9361 tcgtcgtggg tgcgagagtc gccggagcgt ccaccgcgat gctgctcgcg cgggcgggcg
     9421 cgcacgtggc acttgtcgac cgcgctgtct acggctcgga cacggtgtcg acgcacggcc
     9481 tcatgcgagc cggggtgctc cagctgcatc gatgggggct gctcgatcgc atcgtgactg
     9541 ccggcacacc gccggttcga cggacgacgt tccactacga cggcacccgt ggcgagacca
     9601 acgtccagat ctcgatccgc cccagcggtg gtatcgacgc gttgtacgct ccacggcggc
     9661 acgtcatcga tcgtgtgctc gtggacgccg cagccgaggc cggagtggac gtgttgcacg
     9721 agacgcgcgt cgacgacgtc gtgttcgccg atgacggacg agtgtgcggt gtcgccgtgc
     9781 acacccccga tggccggagg gtgctccgat cgcggtacgt cgtaggcgcc gacgggatcg
     9841 gttcgttggt ggcgcgcgcg gtccgtgccg agaccgtgct ggaggggcgt ggtgcgagcg
     9901 ccgtccggta cgcctactac agcgggctcg agccgaccgg gtacgagtgg gcctatgcgg
     9961 atggcgccgc cgcggggatc attcccacga acgacgacgt gtcgtgcgtc ttcgtgtcca
    10021 cgacgccgct tcgcatgcgc acgctgcgga cgaacg
//
LOCUS       DATBFZ010000564        33499 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_5822242, whole genome shotgun sequence.
ACCESSION   DATBFZ010000564 DATBFZ010000000
VERSION     DATBFZ010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 33499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33499
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5822242"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="VH419_17190"
     CDS             <1..378
                     /locus_tag="VH419_17190"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEY7045409.1"
                     /translation="RQSVLAGCALDIDAVIDCYGAWVLTPTDADSTIQVSSLEDRLPR
                     LSCPVLGMFGAEDKHPSPEEVDRFDKVLSELGVEHEFHTFEDAGHGFFGVDRPGYRQA
                     AATEGWRFVRDFLERHLDVKGDR"
     gene            378..716
                     /locus_tag="VH419_17195"
     CDS             378..716
                     /locus_tag="VH419_17195"
                     /inference="COORDINATES: protein motif:HMM:NF039404.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6295 family protein"
                     /protein_id="HEY7045410.1"
                     /translation="MCTYVTEHAAVQGSGKGTDGWFPLTAVTTSYDHPVHAQAEHCVI
                     IDFANLERGPAARVAVELTTASALDLVASIARALVSVPSDLSGVETSDVRRLLDAAEQ
                     VTGAAKVTSW"
     gene            846..2144
                     /locus_tag="VH419_17200"
     CDS             846..2144
                     /locus_tag="VH419_17200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020385820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY7045411.1"
                     /translation="MRRKQLKKTVGLLGVAAFLTTGLAACGGDDSSSGSEDVTLRVNL
                     FGKFGYTDLYKQFEADHPGVKIVETAEGDLGQYNTKLSQWIAAGSGAGDVVAIEEGQI
                     VNFLQKPDNFVNMQDHGSNDMKDQWLPWKYAQATTADGSYTVGLGTDVGGLAMCYRRD
                     LFEKAGLPTDRDEVSALWPTWDDYIDVGKQFMGGIGDDKIHFVDSATNTYNSILMQSG
                     GETYFDHDNNLIIDSNPAVKAAWDESLKMVDAGLSAKLKSFSNEWNAGFKNGAFATVA
                     CPAWMTGYIQDQAGEGNAGKWDIASVPGGGGNWGGSFLAVPSKSDHQDLAIELVKFLT
                     SAKGQLSAFSEVGNLPSNPTVYDDPAVANKTNEYFNNAPVGKIFVQGASQLKPVFLGA
                     ANQPVRDAVENALRSVESGDVSPEDGWDNAVSDAQKAAGI"
     gene            2261..3274
                     /locus_tag="VH419_17205"
     CDS             2261..3274
                     /locus_tag="VH419_17205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020389540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEY7045412.1"
                     /translation="MRTAPTTSPASTETSPGRPGGPDEAAASGSAESSTRWRTRLARW
                     DLRFSPYFFVSPYFILFAIFGLYPLGYTFWVSLHDWALVGNHSWTGFSNYQELLGDED
                     FRNALFNTLGIFILATVPQLLLALVLAHILDKRLRTPLIWRMGVLLPNITSVAAVGII
                     FTLLFARDFGLVNWVLGHLGVDEPIAWQDHRWSSWIAISTMVDWRWTGYNALIFLAAL
                     QAVPKDLYEAAALDGASQFRQFVNVTVPMLRPTLIFVGIISTIGGLQLFTEPLLFNSG
                     ANAITGGTTRQFQTMSMYLVEQAFTRQQFGYAAAIAWVMFLLIGVVAAINIVMLRRIR
                     SAE"
     gene            3284..4171
                     /locus_tag="VH419_17210"
     CDS             3284..4171
                     /locus_tag="VH419_17210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020389539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEY7045413.1"
                     /translation="MVETLLAEHSVARPGLRSSGRRLRQVSPLTYLALVVALLLSAGP
                     LYFMVVMASRPNDEITSIPPPLTPGGQLGANVQRVLDNQDVMFQQALMNSIVVAGITT
                     ISVVVASSLAGFAFAKLRFRGRNALLLVVIATMMVPVQLGFIPLYMLMARLGISGQLS
                     TVVWPFLVSGFGVFMMRQYAIQAIPDELIEAARIDGASTWRIFVSIVFPALRPAAAVL
                     GLLTFMERWNDFLWPYLSLDVDHPTVQVALSRLSSGYYTDQALVMAGTLLGTLPLVVV
                     FIVFGRQIIGGIMEGGVKS"
     gene            4168..5565
                     /locus_tag="VH419_17215"
     CDS             4168..5565
                     /locus_tag="VH419_17215"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020391596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HEY7045414.1"
                     /translation="MTYAQERIVAASSEPDHLAFPHDFVWGAATAAYQVEGSVDADGR
                     KPSIWDAFAAQPGRVVNGDTGEYAADHYRRWREDIGLMQQLGLGAYRFSLSWSRILPD
                     GTGRPNDAGLDFYDRLVDGLLAAGITPWATLYHWDLPVGLESRGGWPVRDTAERFAEV
                     AAASARRLGDRVKNWITLNEPWCSAFLGYSSGVHAPGRTDPAAAVAAAHHLLLGHGLA
                     TAVVREESPASRVGITLNLYPTLPANDSPAAADAARRVDGLQNRWFLDPVLRGRYPED
                     VRNDLREITDFGHERPGDLDVIAAPIDFLGVNYYSSHYVRPGAFPGSNPVEFVDRGRP
                     RTALGWEIDHEGLTTILRRIRADYGPIPLCVTENGAAFDDAVDVDGVVHDQDRVDYLA
                     SHVLACQQAIDDGVPLTAYFVWSLLDNFEWAEGYGSRFGIVHVDFATQQRTIKDSGWW
                     YADFLARHRDGTLRP"
     gene            5562..6581
                     /locus_tag="VH419_17220"
     CDS             5562..6581
                     /locus_tag="VH419_17220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008357326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY7045415.1"
                     /translation="MTTTSGQPTLEEVAALAGVGRGTASRVINGSSQVSDRSRQAVMA
                     AVAELGYVPNPAARSLVTRRTDTVALVISESEDRIFGEPFFAGVIRGISMTITASSRQ
                     LVLALAQTHDETSPLERYLSRQHVDGVLLLSLHGEDPLPRHIRERGLPMVLGGRPVGW
                     DDGGYVDVDNRGGARMGVMHLVERGRRRIATIAGPDDMVAGRDRFDGYCAALQDARLE
                     VDMALVARGDFSQASGSDAMASLLRQAPDIDGVFAANDLMAAGALRILRDAGRNVPHD
                     VSVVGFDDAPMAALTEPPLTTVRQPAEQMGREMATLLIKQITAGAASSDFRILSTQLI
                     KRDSS"
     gene            6659..7186
                     /locus_tag="VH419_17225"
     CDS             6659..7186
                     /locus_tag="VH419_17225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008359203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HEY7045416.1"
                     /translation="MTEQSGDRTPAQEILHDAFDRARELVTDLCDGLTLEQAAFRPSA
                     DTNSIGWLVWHLTRIQDDHICGITGDEQVWTADGWYDRFGLPFRASEHGYGHSSAEVA
                     AVKVDPSLLDGYHGAVHERTLAYVDTVDADELARVVDERWDPPVTASVRLVSVIGDCL
                     QHAGQAAYVRGLLTA"
     gene            complement(7240..8373)
                     /locus_tag="VH419_17230"
     CDS             complement(7240..8373)
                     /locus_tag="VH419_17230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase C-terminal
                     domain-containing protein"
                     /protein_id="HEY7045417.1"
                     /translation="MRLTMGDDKTGTAFVTSGQDFERDTAYITTRVTRDARDGYSVEP
                     GRYRLVVARPCPWANRAVIVRRLLGLEEVMSMGICGPTHDSRSWRFDLDPGGVDPVLG
                     IERLQQAYLARDSGYDKGVTVPALVDIASGQVVTNDFPRLTLDFTTEWVAHHRPGAPD
                     LYPEPLRDEIDSINERVYTDVNNGVYRCGFAGSQKSYDVAFDRLFAALDWLSDRLTAQ
                     RYLVGTTITEADVRLFTTLARFDAVYHGHFKCNRHKLSELPVLWAYARDLFQTPGFGD
                     TIDFVHIKQHYYLVHEDINPTGIVPKGPAGQGWLAPHFRDQLGGRPFGDGTPPGPPPV
                     AERVPADHGVGLPRYDDARPAATEVASAARHADKLQRNLPLRR"
     gene            8601..9410
                     /locus_tag="VH419_17235"
     CDS             8601..9410
                     /locus_tag="VH419_17235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7045418.1"
                     /translation="MADDRHKLELSLPQITGGALAAASAAVASSWLGLAGTVMGAVLV
                     SLVVSIGGALYTHSLQRSSNAIRESLPVVPIRTIRSASREDGAVATTTAVLPRVEEEP
                     DAGKPTDSVPEHQRSRAIRWRTVLITAGASLVLGLGVLTGFELLVGKPASSIVNGNNG
                     GGTTVSRLVSSGQPSNDNPSPADQSPPPGSATTDAPQGSTGGSTSPEPTEPTQPAPTD
                     EQPTPTDPTEPTPTEPTPTEPEPTGATPSQPDSGQAPEGVTEAPTSGAPAV"
     gene            9510..10775
                     /locus_tag="VH419_17240"
     CDS             9510..10775
                     /locus_tag="VH419_17240"
                     /inference="COORDINATES: protein motif:HMM:NF012308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HEY7045419.1"
                     /translation="MVLRTRSRTLVASLAGLSLVTLTACGSQPSDDPGRKESDQVLQS
                     RVAMLPRDASVDFTGTARGLLTLGHDLSTHLPASSGDGGNQVFSPASLAIAFAMLREG
                     ADAASAQEIDDVIGLPENRRAAYNALLHELSDVGPGDRLEINDAVFLDPELEVKPAYL
                     DAIKRWYGAGLHETPFPHPALEDINAWVKTKTHDRIPRLLDDLDASAVLALVNTIYLN
                     AKWETPFSKNATHDAAFTTTEGGTTTVKMMHLNTTLDYATGDGWQAVRLPYAGGSLSM
                     WVLVPDGSGDPKELLAASALATAESSATPTQVRLSLPRWESETKASLVDVLKRLGMGH
                     TFEDGAFPGISDEPRLGVSQVVQQANITVGEKGTEAAAATAIVMELTGVPVLEGVEVT
                     ADHPFAYAIVHDETGVPLFEGVVGNPSAK"
     gene            11086..13551
                     /gene="leuS"
                     /locus_tag="VH419_17245"
     CDS             11086..13551
                     /gene="leuS"
                     /locus_tag="VH419_17245"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017935600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEY7045420.1"
                     /translation="MVEGSEQYDVDAVQAKWLKVWADLDPFKMRDDGSAERRYLLDMY
                     PYPSGDLHMGHAEAWALADVIARWWFQQGYDVLHPVGWDSFGLPAENAAIKRNENPAI
                     WTNANIETQAESFRRYAVSFDWSTRLETHDPAYYKWTQWLFLKLYEHDLAYRKDSPVN
                     WCPNDQTVLANEQVVGGHCERCGAEVTKRDLTQWYFRITAFNQRLLDDMAALEGRWPD
                     RILAMQRNWIGRSEGAHIDFAIGGRDEPVTVFTTRPDTVFGATFFVVAADAPLAAELV
                     TEARRHDFEQYLTEVKRTTEIERLTEGREKSGVFLGVHAVNPATGERIPVYAADYVLS
                     DYGTGAIMAVPGQDQRDWDFAMKFGLPIVRTVQPCDGFDGEAYTGEGPAINSSNDEVS
                     LDGLGVDEAKSRIIGWLESKANGKGTVNFRLRDWLISRQRYWGAPIPIIHCAVDGEVP
                     VPADQLPVVLPELHGGEMQPKGVSPLASATDWVDVECPRCGGPAKRDTDTMDTFVDSS
                     WYFLRYPTPNDATQPFDVEAVRRWGPVDQYVGGVEHAILHLLYARFVTKALHDLGYLD
                     FVEPFTALMNQGEVINQGKGMSKSLGNGVDLGEQIEKFGVDAIRLTLVFAGPPEDAID
                     WADVSPGGSLRFLQRAWRLAGDVTSAPGTDPTGGDAGLRKVTHKVIHDATQLLEIQRF
                     NVVVARVMELVNATRKAIDSGVGPADPSVREAAETVAIILSLVAPYTAEEMWERLGYP
                     PSIARVGWPVADEALLVQDTVTCVVQVQGKVRARLEVSPEASESELEHLAKADENVLR
                     AVGGAEIRKVIIRAPKLVNIVTG"
     gene            14664..15506
                     /locus_tag="VH419_17250"
     CDS             14664..15506
                     /locus_tag="VH419_17250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012920006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEY7045421.1"
                     /translation="MTRSIVLVSDSTSVEPEMARRLDVTVVPLQVIIGATSYDEGVDA
                     GPDDVAAALHRRQPVSTSRPAPEAFLRVYEGAAKQGAEAVVSIHLSGEVSGTFESAQM
                     AARGASIPVHCVDSRQLGMGTGFAVETAAAALDDGISAEKVAEAACERARSTKALFYV
                     DTLEFLRRGGRVGAATAIFGSALAVKPLLAIDDGTIVPLEKVRTTTKAISRLEELAFE
                     AAGDAVVDIAVSHLASADRACDLADRLRAQVPNARGLVVNQVGAVIGAHVGPGLLAVV
                     VAPR"
     gene            15740..16627
                     /locus_tag="VH419_17255"
     CDS             15740..16627
                     /locus_tag="VH419_17255"
                     /inference="COORDINATES: protein motif:HMM:NF024245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HEY7045422.1"
                     /translation="MWGRRTSRTSDTASTARRRLAAIAAEFDRSPVPDQGSSPAPSTP
                     VRPTPAPTTADSEGGRHRGPGGVWDRRRWRLSVHHVAVLTFGAAVLLSLAAWWALRAV
                     PAEEPVPQSDRLGVPTHPSADSPSPGTPSVSTPVDGVPAPAVTAAAAPALVVDVTGKV
                     RRPGIVELSPGSRVVDALAAAGGARPGVDTTGLNLARLLVDGEQIVVGLAIPSMRDTT
                     EAPAVSAPSTGPALGSINVNTASQAELETLPDIGPVTAQAIVDWRRENGSFASVDQLV
                     DVSGIGEATLQDIEPYVHV"
     gene            16624..18993
                     /locus_tag="VH419_17260"
     CDS             16624..18993
                     /locus_tag="VH419_17260"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HEY7045423.1"
                     /translation="MTRDRLDLRLALPALAVWGAAAWGVGWTAVHAVLGALSLLALSG
                     LVAWRGRHRHGADRSGQADRAARQERRDRSAARAQTALAPLLLAAAALTVTALHASSI
                     HAGPVDELAFASSRVEVGGVVASDPEFRIGTFAPYVLVRLRVQLVVATGSQAQVRTPV
                     LVIADDSWRDVRLGDHIVASGRLKPAEGHDLAAVLMADPDPQVVGEASWVQRSVTAVR
                     NGLVEATTPLPSAEHALVVALVDGDDSSMPADTVRDFQTTGLTHLLAVSGSNLTLVLS
                     FVLGLARAMGLRGRWRLALGGLAVVFFVLLARPEPSVLRAAAMGVVALAGLSVGGRRR
                     GMRGLCIAVVCLVLIQPELARSIGFLLSTSATAGILLWAPAWRDQLARWMPAALAEAV
                     AVPLAAQLACTPAIAAISGQVSTVAVIANIAAAPAVGPATVIGLVAGLVALVSEPLAH
                     LVGRLAGIPAWWIVTVSRHGADFAGSSLAWPVGPVAIVALGLLCLLVVLVLPRILASP
                     ATSLACSVLLVLVVVQPLGRLGWPPAGWLLVMCDVGQGDGMVLNAGGGVAVLVDTGPD
                     PRTMDHCLDRLDVKRIDLVILTHFHADHVNGLPGALEGRDVSEIETTSLRDPPDRAAA
                     VDAWAADSGVTVTEATLGESRTIGELTWTVVGPVRTPSDGLSDDGSGPNNASLVILLE
                     THNHSFLLSGDAEPEEEDDILAGHVDLGVDVFKVAHHGSANQSPDFVLSTHARLALVS
                     VGADNDYGHPAPETLALLRRLGAITYRTDLDGDIAVVDRAGQLAVVTAH"
     gene            complement(18994..20286)
                     /locus_tag="VH419_17265"
     CDS             complement(18994..20286)
                     /locus_tag="VH419_17265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020579126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY7045424.1"
                     /translation="MTWKERLARVRVDTTPLRESRDFRLLFWAGTVFYLGGMVSYVAV
                     PYQLYQLTGSNFAVGALGIVELVPLVVFGLWGGALADHMDRRIMLVSTGMAQILLTSC
                     LMINAFMPSPQVWPLYAIGALLSAAQALQRPSWEALIPRTVKHAQLPAAVSLSSFGMQ
                     IGVLGGPAAGGVLLQWVGVPWCFAVDVSGLLVATALFALLRPYPALDTSNPPSVRGIM
                     EGIGYAVRRRDLLGTYIIDIVAMLMAMPTVLFPAFAQDVLKTPSVLGLLYTAGTVGGL
                     LATATSGWTSRVHHHGRAVVIAAAAWGAAIALVGVSSSVLFILFWLTVAGAADMVSGI
                     FRDVIWHQTIPDSMRGRLAGIEMLSYSLGPLGGQTRAGLVADRWSVRAAITTGGVLCV
                     IGVAATAAWLRDFWSYDARTDEHAVRERLVREAAANAG"
     gene            20397..21383
                     /gene="holA"
                     /locus_tag="VH419_17270"
     CDS             20397..21383
                     /gene="holA"
                     /locus_tag="VH419_17270"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020388464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEY7045425.1"
                     /translation="MSLGTSTDAVLATTTLITGGDEFLAERAIAGLKTVVRAADVDAD
                     VAELEAAALGVGALAEIASPSLFAANRCVVVRRIEELADEAVEPLLAYVAEPSPDVAL
                     VLHHTGGVKGKATLDKLRKAGAREIAAQGPKRWELPTWVQREFRSHGKKVEKAAAAAL
                     VDALGDDLRSLAGAASQLAVDCPDDTITAQTVRAYFGGRADVKGFAVADAAIDGKGAE
                     AIEQLRWALGGRSPAPVLITSAMAAGLRSIARFQAAPRGLREGDLAREVGVPPWKLKS
                     IAAQSRGWSARGLAAAIQAAAQADADVKGAGGDPLWACERLVISVIRARALR"
     gene            complement(21525..21785)
                     /gene="rpsT"
                     /locus_tag="VH419_17275"
     CDS             complement(21525..21785)
                     /gene="rpsT"
                     /locus_tag="VH419_17275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012920011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEY7045426.1"
                     /translation="MANIKSQIKRIKQNNAAHERNKSVKSALKTSIRRFREAAEAGET
                     EQATELAKAASRKLDKAASAGVIHKNQAANKKSAIAKKAASL"
     gene            22001..23401
                     /locus_tag="VH419_17280"
     CDS             22001..23401
                     /locus_tag="VH419_17280"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF006006.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEY7045427.1"
                     /translation="MTSGALVEQALDRIDSIDPLINAVCTLNPAALDDAAARDAEEGD
                     GRSRGPLHGVPILVKDNIDTAGLATTAGSLALVDIPPPATDATLVQRLRDAGMVVIGK
                     ANLSEWANLRDERSTSGWSAYGGLTRNPYGLNRSAGGSSSGSGAAVAAGLVRYAVGTE
                     TDGSITCPAAFNGCVGIKPTVGLVPTDGVVPISSSQDSPGPLNATVGEAAALLGVLTG
                     EGPDHYTRRLDEGLRGQRIGVPRKVFWGYSSQADEIAEEALALLSGAGAVIVDETDLD
                     SMVDFGFDDELAVLLAEFEVGLGAYLRSREGDGPKSMADLVAFNREHADTELAWFGQE
                     YLEKALIAPGPDSPEYAEARAKCLKAGRDDGIDAVLREHELDALVTPSYAPAIPIDLV
                     NAERHHGSCTQPSAMAGYPILTVPAGLSQGLPVAISFWGAARSEPALLAMGSGYERAR
                     DAAAGELASPTFPQFV"
     gene            23411..24100
                     /locus_tag="VH419_17285"
     CDS             23411..24100
                     /locus_tag="VH419_17285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7045428.1"
                     /translation="MLRALLYGLGTAGPLVAGAAAGLWWKLPDRVLAALMAFGAGTMI
                     AAVSSELFQPAFNELGGGWASIALLGGAAVYVVADRLIELRLGPASLGWALMLGTVLD
                     GVPENTALGVSLTETGGVVLLVAIAVGNLPEAISGSAQMRRHHSLNPGHIVLLWVATG
                     AVLVAVTVVGYAASDVVRPASIAAVQAIAGGATIAVLSDSLIPEAYREGGWWIGLATA
                     FGFVVAFSLGA"
     gene            complement(24134..24955)
                     /locus_tag="VH419_17290"
     CDS             complement(24134..24955)
                     /locus_tag="VH419_17290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEY7045429.1"
                     /translation="MAHVCVGISGWVYPAWRGTFYPKGLAQKRELEYAAERLTSIEIN
                     GTFYSLQRPSSFGRWHDETPDEFVFAVKGPRFITHMKRLADPVSPLANFFASGVLALG
                     RKLGPVLWQLPPTFGFDEARLVAFFEQLPRTTSAAARLSKHHDERITEDRAFTTASVD
                     IPVKHALEVRHESFVTDRSLDLLREHDVAIVVADTAGRWPMIDAVTSDFVYVRLHGAD
                     ELYVSGYTPDALDRWAERVRGWFGDGLDVYVYFDNDAKVRAPYDALGLIERLDQR"
     gene            complement(24962..25825)
                     /locus_tag="VH419_17295"
     CDS             complement(24962..25825)
                     /locus_tag="VH419_17295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007078340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEY7045430.1"
                     /translation="MSDGTVKPPASTTSVLITGCSTGIGRATATRLARRDDLQVYATA
                     RRLETIDDLREADCRTLALDVTDEDSMAAAVAAVEEQTGSVGVLVNNAGYSQSGAVET
                     VPMDEVRRQFETNVFGLVRMCQLVLPGMRRAGRGRIVNVSSMGGRLTFPGGGIYHGTK
                     YAVEAISDVLRFEVRGFGVDVCIIEPGFIRSSFADTAVESVTQEDGPYAEYNAAVARS
                     TAGIYHSPLGRLGGAPEAVAKAIEKAVTSRRPRTRYPVTPSARLFMGQHALLPDRAWD
                     AVVGSSFPRPS"
     gene            25960..27111
                     /locus_tag="VH419_17300"
     CDS             25960..27111
                     /locus_tag="VH419_17300"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF010041.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEY7045431.1"
                     /translation="MTETGEGRQVGVEEELMLVDPETGRLRAVSDRALAAHRDVRSDE
                     ADDEAFGASGGLAQELFLVQIETGTQPTRDIAELVADVRRCRQSAAESARSAGAELVA
                     TATAVIGGEDREVTRKTRYERIVGTFGEISRQGSVCGMHVHVEVRDAAEAVKVIDRLR
                     PWLPVLRAMSVNSPFYYGVDTGYASWRTQIWSRWPTAGPCEPYGDLSGYRQATDALIA
                     SGAALDRGMLYLDARVSEHYPTIEIRVFDVVSEPTDVGLLAALTRALVDTLAERSDGP
                     VWRSDLTKAAHWLASRDGLSRDLVEPFSAAPVSARQVIERMIDHCGESLRRAGDLDRV
                     SEGLDLLLARGTGATQQRAAAERTGSMDGVVADLRERFTASLSEPRQAN"
     gene            complement(27127..27549)
                     /locus_tag="VH419_17305"
     CDS             complement(27127..27549)
                     /locus_tag="VH419_17305"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY7045432.1"
                     /translation="MTAVMHARRKIFVNLPVNDLADSVAFFSKLGFRFNPEFTDEQAA
                     CMVVSDDAFVMLLPRDFYASFTTKSVVDSTTHNEVILAISADSRDEVDRLVARALESG
                     GAPSAAKIVDGPMYGWSFADVDGHLWEVLYMDPADVAR"
     gene            28154..28531
                     /locus_tag="VH419_17310"
     CDS             28154..28531
                     /locus_tag="VH419_17310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012924489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY7045433.1"
                     /translation="MFAESKAFSGFAVDDVDAAKRFYGEVLGLTVNAVDDEGQLLQLE
                     LAGGTNVFVYGKPGHVPATYTILNFPVDDVERAVDALTANGVAMLRYDGFGQDEKGIA
                     RGNGPDIAWFSDPSGNILSVLST"
     gene            28578..29594
                     /locus_tag="VH419_17315"
     CDS             28578..29594
                     /locus_tag="VH419_17315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012918204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY7045434.1"
                     /translation="MSQDLLASTDEPSTPDDDFPRLVDPYRRELLAHCYRMMGSIHDA
                     EDLVQETYLRAWRSYHAFENRSSLRTWLYRIATNACLTALDGKSRRPLPTGLGAPSSS
                     PDEPLVEQGEVPWLEPVPDVMLGNDPNDPASIVTSRESIRLALIAALQYLPPRQRAVL
                     VLRDVLRWKAAEVADALETSTAAVNSILQRARAQLERADITEDSIVEPESAEQRELLD
                     RWVDAFWEKDVAAIVRMFSEDAVWEMPPYTGWYRGPEVIGRLIDTKCPGDVHDMRMLP
                     TRANGQPAFALYMRTPSGEFEPFHLQVLTIGPNGVRHVAAFFDHALFERFGLPARLPA
                     ESRA"
     gene            29606..30226
                     /locus_tag="VH419_17320"
     CDS             29606..30226
                     /locus_tag="VH419_17320"
                     /inference="COORDINATES: protein motif:HMM:TIGR03086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03086 family metal-binding protein"
                     /protein_id="HEY7045435.1"
                     /translation="MPFDLSAGRSGRTLMPELDLLERAVAYTRLSLQLVAPDVLHAPT
                     PCEEWDLVTLLGHMDDSLAALADAADVGYVAIRPLPRPDPANAVTSIKARACALLAAW
                     TAGGGADLVSVAGCPLSARVLVSTGALEITTHGWDVARACGVDRQIPGALAQVLLRML
                     PDLVHDSDRVTRFAHAVDVPPWWSPSDRLVAALGRQPVWSAHSRPV"
     gene            complement(30399..31340)
                     /locus_tag="VH419_17325"
     CDS             complement(30399..31340)
                     /locus_tag="VH419_17325"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEY7045436.1"
                     /translation="MADGVGERLTGVRTAYDDIPGPLHSWIEDQLGGQVSTTIPRTGG
                     MSPAVAATVVVADGGRAFVKAVSSDINPDTPTHFRHEIDVLSRIGPAPYRADLLGTYD
                     DGHWVAMLLEDIDGDHPDWDDESQIEAVFTAVRQQADELTPMPDGLDTVSVAEILEKH
                     QRMLLADPPAQLFARLPAWARQDYADLTAMVRDGRPVHGDTLCHWDVRHDNLLVRRTD
                     HQVVLLDWGMARQGPWWGDVLVLGLEWADTGRFDDLLDRAGLDESEQHDATRVLASVG
                     MFLTMSSGLPPPPGLPHLPTFRADLGNRCLAGVRRRW"
     gene            complement(31333..32424)
                     /locus_tag="VH419_17330"
     CDS             complement(31333..32424)
                     /locus_tag="VH419_17330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7045437.1"
                     /translation="MNAGRSDATRGYPAAAPAVAAPIDDHQWQNDSVMLPTGVVVPVR
                     ATARRRTWQGLPAVVQTAIEYAAGSPVVTATTTGTGFTAGFASRLDLADGSACFVKAA
                     STRDDRLHGWQISSAYRIEARNLRLLPSDVGAIPLSWSLDVDDEDDAWIVLGFPYIHG
                     HPPRRPWRPRELRLVTDKLAQVAPALARAPQGLDLEPYDGDRDQLPAWIARVLERDGE
                     SRWLDAIIGLAGESVTCTQGTAVAHLDLRDDNIVLGDDGSVWICDWNWPMLAAPWLDL
                     VTVLIAAYGDGLDTDTALCDHPLTAALDPRSVDAWLANLWLYLTTRMEGDVPAGSPHL
                     RDHQAWYEDVTRRWLEQRIGISARVDDHG"
     gene            32720..>33499
                     /locus_tag="VH419_17335"
     CDS             32720..>33499
                     /locus_tag="VH419_17335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015070108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY7045438.1"
                     /translation="MANPSKTIASPPQPGRTAPSIIRNFCIIAHIDHGKSTLADRMLQ
                     LTGVVDARSMRAQYLDRMDIERERGITIKSQAVRLPWTVSDGDAAGTTYVLNMIDTPG
                     HVDFTYEVSRSLAACEGAVLLVDAAQGIEAQTLANLYLALDADLDIIPVLNKIDLPSA
                     QPEKYAAELAHIIGCDADDVLRVSAKTGAGVTELLDKIVASVPAPRGVAEAPPRALIF
                     DSVYDTYRGVVTYVRVVDGKLSHRDRIKMMSTGAVHEMLEVG"
BASE COUNT         5318 a        11315 c        11547 g         5319 t
ORIGIN      
        1 cggcagtcgg tgctggccgg ttgcgcgctg gacatcgacg cggtgatcga ctgctacggg
       61 gcctgggtgt tgacaccgac ggatgcggat tcaaccatcc aggtgtccag cctggaggac
      121 cggcttcccc gcctgtcgtg cccagtcctc ggcatgttcg gggcggagga caagcacccc
      181 tccccagagg aggtcgaccg tttcgacaag gtcttgagcg agctgggcgt cgagcatgag
      241 ttccacacct tcgaggacgc cgggcacggc ttcttcggcg tcgaccggcc gggctaccgg
      301 caggcggctg ccaccgaggg ctggcggttc gtccgcgact tcctcgagcg tcacctcgac
      361 gtgaagggcg accgctgatg tgcacctacg tgaccgagca cgcggcggtc caggggagtg
      421 gcaagggcac cgacggctgg ttcccgctga ccgccgtcac cacgtcgtac gaccatccgg
      481 tgcacgctca ggccgagcac tgcgtcatca tcgacttcgc caacctcgag cgcggtcctg
      541 ccgcgcgggt ggccgtggag ttgaccaccg cctcggcact cgacctggtc gcgtcgatcg
      601 cgcgcgccct cgtctcggtg ccgagcgacc tttcgggtgt cgagacctcc gacgtccggc
      661 ggctgctcga cgccgccgag caggtgaccg gggcggcgaa ggtcacaagt tggtaacggt
      721 catgggagcg ttcccaaatc gctcttgacg cgatgtgacc tgcggggcca ctgttcgtgg
      781 gaacgctccc acgaatcaga ctgggggcac accgagagcg tgagggctcg cgaaatgagg
      841 tcgctgtgag gcgcaagcaa ctcaagaaga ctgtggggct gctcggagtg gcggccttcc
      901 tgaccaccgg acttgcggcg tgcggcggtg acgactcgag ctccggctcc gaggacgtga
      961 cgctgcgcgt caacctgttc ggaaagttcg gttacaccga cctgtacaag cagttcgagg
     1021 ccgaccaccc cggcgtgaag atcgtggaga ccgccgaggg cgacctgggc cagtacaaca
     1081 ccaagctgtc ccagtggatc gccgccggca gtggcgccgg tgacgtggtg gccatcgagg
     1141 aggggcagat cgtcaacttc ctccagaagc ccgacaactt cgtgaacatg caggaccacg
     1201 ggtccaacga catgaaggac cagtggctgc cctggaagta cgcacaggcg acgacggccg
     1261 atggttccta caccgtcggg ctcggaaccg acgtcggcgg gctggcgatg tgctaccggc
     1321 gtgacttgtt cgagaaggcc ggcctcccga cggatcgtga cgaggtcagc gcgttgtggc
     1381 ccacctggga cgactacatc gacgtcggca agcagttcat gggcggcatc ggcgacgaca
     1441 agatccactt cgtcgactcg gcgacgaaca cgtacaactc gatcctcatg cagtccgggg
     1501 gcgagacgta cttcgaccac gacaacaacc tgatcatcga ctcgaacccg gccgtcaagg
     1561 cggcgtggga cgagtcgctc aagatggtcg acgcgggtct gtcggcgaag ctcaagtcct
     1621 tctccaacga atggaacgcc ggcttcaaga acggcgcgtt cgcgaccgtg gcctgcccgg
     1681 cgtggatgac cggctacatc caggaccagg ccggtgaggg caacgcgggc aagtgggaca
     1741 tcgcatccgt gcctggtggc ggtggcaact ggggcggctc gttcctcgcg gtcccgtcca
     1801 agagcgacca ccaggacctg gcgatcgagc tcgtgaagtt cctgaccagc gcgaagggcc
     1861 agttgtcggc gttcagcgag gtcggcaacc tgccctccaa cccgacggtg tacgacgacc
     1921 cggcggtggc gaacaagacg aacgagtact tcaacaacgc accggttggc aagatcttcg
     1981 ttcagggagc aagccagctg aagccggtgt tcctcggagc cgcgaaccaa ccggtccgtg
     2041 acgccgtgga gaacgccctg cgcagcgtcg agtccggcga cgtctcgccc gaggacgggt
     2101 gggacaacgc ggtgtccgac gctcagaagg ccgccggcat ctgacccggg cctgaagcgc
     2161 acccgacgag cctcgggtgc ccgcggtacc cctgatcgcg ggcacccgtg cgctatcgga
     2221 cgtgcgccgg accatcggcg acgagcgcga aggaggaggt atgaggacag cgcccacgac
     2281 gtcacccgcg tcgacggaga cgtcgcccgg ccggccgggc ggccccgacg aggccgcggc
     2341 cagcggctcc gccgagtcgt cgacccggtg gcggacccgg ctggctcgct gggacctcag
     2401 gttctcgccg tacttcttcg tctcgccgta cttcatcctg ttcgcgatct tcgggctcta
     2461 tccgctgggc tacacgttct gggtgtcgct ccatgactgg gcgctggtcg gcaaccacag
     2521 ctggaccggg ttcagcaact accaggagct gctgggggac gaggacttcc ggaacgcgct
     2581 gttcaacacc ttggggatct tcatcctcgc gaccgtgcct cagctgcttc tggcactcgt
     2641 gctcgcgcac atcctggaca agcggctccg gacgccactg atctggcgaa tgggcgtact
     2701 gctgccgaac atcacgtcgg tggcagcggt cggcatcatc ttcacgttgc tcttcgcccg
     2761 cgacttcgga ctggtgaact gggtcctcgg ccatctcggc gtcgacgagc cgatcgcctg
     2821 gcaggaccac cggtggtcct cctggatcgc gatctcgacc atggtcgact ggcgctggac
     2881 gggatacaac gcactgatct tcctcgccgc actccaggcc gtgcccaagg acctgtacga
     2941 ggctgccgcg ctggacggtg cgtcgcagtt caggcagttc gtgaacgtca ccgtcccgat
     3001 gctgcggccc acgctcatct tcgtcgggat catctccacg atcggtggcc tgcagctctt
     3061 caccgagcca ctgctcttca actccggtgc gaacgcgatc accggcggca ccacacggca
     3121 gttccagacc atgagcatgt acctggtcga gcaggcgttc acgcgtcagc agttcggcta
     3181 cgccgcggcg atcgcgtggg tcatgttcct cctcatcggc gtcgtcgctg cgatcaacat
     3241 cgtgatgctc cgacgcatca ggtcggcaga gtaggcggcg gacatggtcg agacattgct
     3301 cgccgaacac agcgtcgcgc gacccggcct ccggtcgagt gggcgccgac ttcggcaggt
     3361 gagcccgctg acgtacctgg cgctcgtcgt ggcgctgctg ctgtcggccg gaccgctcta
     3421 cttcatggtg gtgatggcgt cgcgtcccaa cgacgagatc acctcgatcc cgccgccgct
     3481 gacgcctggt ggccagctcg gcgcgaacgt gcaacgcgtc ctcgacaacc aggacgtgat
     3541 gttccagcag gcgctgatga acagcatcgt cgtcgccggc atcacgacga tctccgtggt
     3601 cgtcgcttcg tcactggccg ggttcgcctt cgcgaagctg cgcttccgcg gccgcaacgc
     3661 actgctgctc gtcgtgatcg cgaccatgat ggtgccggtc cagctcggct tcatcccgct
     3721 ctacatgctg atggcgaggc ttgggatcag cggacagctc tcgacggtcg tctggccgtt
     3781 cctcgtgagc ggtttcgggg tcttcatgat gcgtcagtac gccatccagg ccatccccga
     3841 cgagctcatc gaggcggccc ggatcgacgg tgcgtcgacc tggcggatct tcgtctccat
     3901 cgtctttccg gcgctgcgcc cggcggccgc ggtgctcggt ctgctcacgt tcatggagcg
     3961 atggaacgac ttcctgtggc cgtacctgag cctcgacgtc gaccatccga ccgtgcaggt
     4021 ggcgctgtcg cggctttcga gtggctacta cacggaccag gcgctggtga tggccggcac
     4081 gctgctgggc acgctgccgc tggtcgtcgt gttcatcgtc ttcggtcggc agatcatcgg
     4141 cgggatcatg gaaggcggag tgaagtcatg acctacgcgc aggagcggat cgtcgcggct
     4201 tcttccgagc ccgaccacct cgcatttccg cacgacttcg tctggggcgc ggccacggcg
     4261 gcgtaccagg tcgaaggttc ggtcgacgca gacggcagga agccgtcgat ctgggacgcg
     4321 ttcgcggcac agccgggccg cgtcgtcaac ggtgacaccg gcgagtacgc cgctgaccac
     4381 taccggcggt ggcgcgagga catcgggctg atgcagcagc tcggactggg cgcctaccgg
     4441 ttttcgctgt cctggtcgcg gatcctgccc gacggcaccg gtcgcccgaa cgatgcaggg
     4501 ctggacttct acgaccggct cgtcgacggt ctgctcgccg ccggcatcac accgtgggcg
     4561 accctttacc actgggacct gccggtcggg ctcgagtcga ggggcggctg gccggtccga
     4621 gatacggccg agcggttcgc cgaggtcgcc gcggcgagtg cgcgccggct cggcgatcgg
     4681 gtgaagaact ggatcacgct caacgagcct tggtgctcgg cgtttctcgg ttactccagc
     4741 ggtgtgcacg cgccgggacg gaccgaccct gctgctgcgg tcgccgccgc acaccacctg
     4801 ctgctgggac acggactcgc gacggccgtg gttcgtgagg agtcgccagc cagccgggtc
     4861 ggcatcacgc tgaacctgta cccgacgctg cccgccaacg actcgccggc cgcagcagac
     4921 gccgctcgcc gggtcgacgg gctgcagaac cgctggttcc tcgaccccgt gctccgcggt
     4981 cgttatcccg aggacgtccg gaacgacctg cgcgagatca ccgacttcgg ccacgaacgt
     5041 ccaggcgacc tggacgtgat cgccgcgccg atcgacttcc tcggcgtcaa ctactactcg
     5101 agccattacg tgcgtcccgg cgccttcccc gggagcaatc ccgtcgagtt cgtagaccgt
     5161 ggacgacccc gaaccgcgct gggctgggag atcgaccacg agggcctgac gacgatcctg
     5221 aggcggatcc gggcggacta cggacccatt ccgctgtgcg tgaccgagaa cggcgctgcg
     5281 ttcgacgacg ccgtcgacgt cgacggggtg gtgcacgacc aggaccgggt cgactatctc
     5341 gcgtcgcacg tcctcgcctg tcagcaggcg atcgacgacg gggtgccgct gacggcgtac
     5401 ttcgtctggt cgctgctcga caacttcgag tgggcggaag ggtacggcag caggttcggc
     5461 atcgtgcacg tggacttcgc cacccagcag cgcacgatca aggacagcgg ttggtggtat
     5521 gccgacttcc tggcccgcca tcgcgacggg acactgcgac catgacgacg accagtgggc
     5581 agccgacact tgaagaggtg gccgctctcg caggtgtcgg ccgcggcacc gcgtcgcgtg
     5641 tcatcaacgg gtcgtcgcag gtgtccgacc gcagcagaca ggccgtgatg gccgcagtcg
     5701 ccgagctcgg ctatgtcccc aacccggcag ccaggtcgct ggtgacgcgc cggacggaca
     5761 ccgtggcgct ggtgatctcc gagtccgagg accgaatctt cggcgagccg ttcttcgccg
     5821 gcgtgatccg ggggatcagc atgacgatca cggcctcctc gcggcagctc gtgttggcgc
     5881 tggcacagac ccatgacgag acgagcccgc tcgagcggta cctgtcacgt cagcacgtcg
     5941 acggcgtact cctgctctcc ctgcacggcg aagatccgct gcctcggcac atccgtgaac
     6001 gcggcctgcc gatggtgctc ggtggccggc cggtcggatg ggacgacggc ggctacgtcg
     6061 acgtggacaa ccgtggcggc gcacggatgg gcgtgatgca cctcgtcgag cgcggccggc
     6121 gccgcatcgc caccattgcc ggtcccgacg acatggttgc cggtcgggac cggttcgacg
     6181 gttactgcgc ggcgctgcag gacgctcgtc tcgaggtcga catggcactc gtggcgcgcg
     6241 gcgacttcag ccaggcgagc gggagtgacg cgatggcatc gctgctgcgg caggcacccg
     6301 acatcgacgg cgtgttcgcg gcgaacgacc tgatggcggc cggcgcgctg cgcatcctgc
     6361 gcgacgccgg ccgcaacgtc ccacatgacg tctccgtggt cggcttcgac gatgcgccga
     6421 tggccgcgct caccgagccg ccgctgacga cggtgcggca gcccgccgag cagatgggtc
     6481 gtgagatggc gacgctgctc atcaagcaga tcacggcagg cgcggcgtcc tcggacttcc
     6541 gcatcctgtc cacccagctc atcaagcgcg actccagctg agcggtccac tcgcggatgt
     6601 cggcgcaggg tgcttgagtt gtctccggcc tgggtgacgc gactcgacag gagcaacgat
     6661 gaccgaacag tccggcgacc gcactcctgc ccaggagatc ctgcacgacg ccttcgatcg
     6721 cgcccgtgag ctggtgacgg acctgtgcga cggcctgaca ctcgagcaag ccgccttccg
     6781 cccgtccgcg gacaccaact cgatcggctg gctggtctgg catctgacgc gcatccagga
     6841 cgaccacatc tgcggcatca cgggcgacga gcaggtgtgg accgcagacg ggtggtacga
     6901 ccggttcggc cttccgttcc gcgcgtcgga gcacggctac gggcacagct cggccgaggt
     6961 ggccgcagtg aaggtggacc cgtcgctgct cgacggctac cacggcgctg tccacgagcg
     7021 gacactcgcg tatgtcgaca cagtcgatgc cgacgagctg gcccgtgttg tcgacgagcg
     7081 ctgggaccca ccggtcacgg ccagcgtccg gctggtgagc gtgatcggcg actgcctcca
     7141 gcacgccggc caggcggcgt acgtcagagg tctcctcacc gcctgacggt tggttgtcgg
     7201 acccgaggtc cctgtgcgac agcgcggacc cgccagcgtt caacgccgaa gtggcaggtt
     7261 tcgttggagt ttgtcggcgt gtcgcgcagc gcttgccacc tcggttgcgg cgggtcgggc
     7321 gtcgtcgtac cgcgggagac cgacaccatg gtcggcgggc actcgctcgg ccaccggagg
     7381 cggcccaggt ggcgtaccgt ccccgaacgg tcggccgccg agctggtcgc ggaagtgcgg
     7441 cgccagccac ccttgcccgg ccggcccctt gggaacgatt ccggtcgggt tgatgtcctc
     7501 gtgcacgagg tagtagtgct gtttgatgtg cacgaagtcg atcgtgtcgc cgaagcctgg
     7561 tgtctggaac aggtcgcggg cgtacgccca cagcacgggt agctcgctga gcttgtggcg
     7621 gttgcacttg aagtgcccgt ggtagacggc gtcgaaccgc gcaagcgtcg tgaacagccg
     7681 cacgtcggcc tcggtgatgg tcgtgccgac caggtaccgc tgagcggtga gccggtcgga
     7741 cagccagtcg agcgcagcaa acagacggtc gaacgccacg tcgtacgact tctgcgaccc
     7801 ggcgaaacca cagcggtaca ccccgttgtt gacgtcggtg tagacccgtt cgttgatcga
     7861 atcgatctcg tcgcgcagcg gctccggata caggtccggg gcgccgggtc ggtgatgcgc
     7921 gacccattcc gttgtgaagt ccagcgtcag tcttgggaag tcgttggtca cgacctgtcc
     7981 gctcgcgatg tccacgagag ccggaacggt gacgcccttg tcgtaaccgg agtcgcgcgc
     8041 gaggtaggcc tgctgcagcc gttcgatgcc gagcacggga tcgacgccgc ccgggtcgag
     8101 gtcgaaccgc cagctccggc tgtcgtgcgt cggaccacag atccccatcg acatgacctc
     8161 ttccagccca agcaggcggc ggacgatcac cgcacggttg gcccatgggc acggccgcgc
     8221 gacgacgagg cggtagcgac caggttcgac ggagtagccg tcacgcgcgt cgcgtgtcac
     8281 tcgggtcgtg atgtacgccg tgtcgcgctc gaagtcctgc ccggacgtga cgaacgcagt
     8341 accggtcttg tcgtcgccca tcgtcagcct catctcgtcg gtccgtcgta ctgtgcccgg
     8401 gcccggcgag aacatgcccc gcaccacctg gtgagccggc agtgcgtgtg gccggagtca
     8461 ccatccagat ggcggttcgc gtttgagtct gttcggcaac cctcgatagc ctggaggggt
     8521 cctcatggga cgcgacaacc ccacaaccca gacgtccgca acgggcgtct gactcacgtt
     8581 ggaaggaacc tgaccgcacg atggccgacg accgtcacaa gctcgagctg agcctgccgc
     8641 agatcaccgg tggtgcgctg gccgctgcct ctgcagcggt cgcctcgtca tggctggggc
     8701 tggccgggac cgtcatgggc gctgtgctgg tcagccttgt cgtctcgatc ggcggcgccc
     8761 tgtacacgca ctccctccaa cgcagctcga acgcgatccg cgagtccctc cccgtcgtgc
     8821 cgatccgcac catcaggagt gcctcccgcg aagacggtgc agtcgcgacc acgacggcgg
     8881 tgctgccgcg cgtcgaggag gagcctgacg ccgggaagcc gaccgactcg gtgccggagc
     8941 accagcgcag ccgcgcgatc cgctggcgca ccgtcctgat caccgccggc gccagcctgg
     9001 tgctcggcct cggcgtgctg accgggttcg agctcctcgt cgggaagccc gcgtcgtcga
     9061 tcgtcaacgg caacaacggt ggcggcacca ctgtctcccg gctggtgtcg agcggccagc
     9121 cgagcaacga caacccgtct ccggctgacc agagcccacc tccaggctcc gcgacgaccg
     9181 acgctcccca gggcagcacc ggtggcagca cgtcgccgga gccgaccgag ccgacgcagc
     9241 cggctccgac ggacgagcag ccgaccccga ccgacccgac cgagccgacg ccgaccgagc
     9301 cgacgccgac cgagcccgaa ccgacagggg cgacgccgtc gcagcccgac tccggccagg
     9361 ctcctgaagg ggtgaccgag gcgccgacgt caggagcccc ggccgtctga caacgcggcg
     9421 tacgccggac gagcgggcgg aaccgaaaac gtcggagcgc cgtctcacca ccacgtgact
     9481 catcgtcacc ctcgacgaag gagaccctcg tggtgctgcg cactcggtcc cgtacgctgg
     9541 tggcctcgct ggccgggctg tccctcgtca cgctgacggc gtgcggcagc cagccgagcg
     9601 atgatcccgg gcggaaggag agcgaccagg tgctgcagtc ccgggtggcg atgctgcccc
     9661 gcgacgcctc ggtcgacttc accggcaccg cccgagggct cctcacgctg ggacatgacc
     9721 tgtccaccca ccttcccgcc agctcgggag atggcggcaa ccaggtcttc tccccggcca
     9781 gtctcgccat tgcgttcgcg atgctccgcg aaggtgccga cgcggcaagt gcccaggaga
     9841 tcgacgacgt catcggactg cccgagaacc gtcgcgcggc gtacaacgca ctgttgcacg
     9901 agctttcgga cgttggcccg ggagatcggt tggagatcaa cgacgccgtg ttcctcgacc
     9961 cggagctcga ggtcaagccg gcgtacctcg acgcgatcaa gcggtggtac ggcgccgggc
    10021 tgcacgagac gccgttcccg cacccggcgc tggaggacat caacgcctgg gtcaagacga
    10081 agacccacga ccgcatcccg cggctgctcg acgacctcga cgcgagcgcc gtactcgcgc
    10141 tggtcaacac gatctacctc aacgcgaagt gggagacacc gttcagcaag aacgccacac
    10201 acgacgcagc cttcacgacc accgagggtg ggacgaccac ggtcaagatg atgcatctga
    10261 acacgacact cgactatgcg accggcgacg gctggcaggc cgtccgactg ccgtacgcgg
    10321 gcgggagtct ctcgatgtgg gtcctcgtcc cggacggttc gggtgatccg aaggagctgc
    10381 tggcggcgag tgcgctcgcc acagccgagt catcggcgac gcccacgcag gtgaggctga
    10441 gcctgccgag gtgggaaagc gagaccaagg cgagtctcgt cgacgtcctg aagcgactcg
    10501 gcatggggca cacgttcgag gacggtgcgt tccccggcat cagcgacgag ccacggttgg
    10561 gcgtctccca ggtcgtacag caagcgaaca tcacggtcgg cgagaagggc acggaagcag
    10621 ccgcggcgac ggcgatcgtc atggaactga cgggagtgcc ggttctcgaa ggcgtcgagg
    10681 tcacggctga tcacccgttc gcctacgcga tcgtccacga cgagaccggc gtgcctctct
    10741 tcgaaggcgt cgtcggaaac ccgtccgcga agtagacgca gccgacgggc acacgcgcgg
    10801 aacggcatcg cgggacgtgt gacgcctgcg ctaccctgtc tgcatgcaga agttcctgct
    10861 ccttagatgg ccgcgctgat cacagctcaa cgacagcgcg gctgcccctc atggctgagg
    10921 ggcttttcta tgtccgcatg acgggcagtc tcgatgtgac ccatcgacgg cacccgcgac
    10981 cggcgtacgt ctgaccgacg cgaccggtga accaccatct tcgagcaggc aaccccgcac
    11041 aggagagcag tgatgagcga gcagacggac cgggccgacc aacgcatggt cgagggatct
    11101 gagcagtacg acgtcgacgc cgtccaggcc aagtggctga aggtctgggc cgacctcgac
    11161 ccgttcaaga tgcgcgacga tggcagcgcc gagcgccgct acctgctgga catgtacccg
    11221 tacccgtcgg gtgacctgca catgggacac gccgaggcgt gggcgctggc cgacgtgatc
    11281 gcgcgctggt ggttccagca ggggtacgac gtgctgcacc cggtcggctg ggactcgttc
    11341 gggctgccgg cggagaacgc cgcgatcaag cgcaacgaga acccggcgat ctggaccaac
    11401 gccaacatcg agacgcaggc ggagtcgttc cggcggtacg ccgtcagctt cgactggtcg
    11461 acccgcctgg agacgcacga cccggcgtac tacaagtgga cgcagtggct gttcctgaag
    11521 ctgtacgagc acgacctggc gtaccgcaag gactcgccgg tcaactggtg cccgaacgac
    11581 cagaccgtgc tggccaacga acaggtggtc ggtggccact gcgagcgttg cggcgccgag
    11641 gtgaccaagc gcgacctgac ccagtggtac ttccgtatca ccgcgttcaa ccagcggctg
    11701 ctggacgaca tggcggcgct ggagggcagg tggccggacc gcatcctggc gatgcagcgc
    11761 aactggatcg ggcgttctga gggcgcccac atcgacttcg ccatcggggg ccgcgacgag
    11821 ccggtgacgg tcttcacgac gcggccggac acggtgttcg gtgcgacgtt cttcgtggtc
    11881 gcggcggacg ccccgttggc cgccgagctg gtgaccgagg cgcggcgaca cgacttcgag
    11941 cagtacctga ccgaggtcaa gcggacgacg gagatcgagc ggctgacgga gggccgtgag
    12001 aagtccggcg tcttcctggg tgtgcacgcg gtcaatccgg ccaccggcga gcggatcccg
    12061 gtgtacgccg cggactacgt gctgtccgac tacgggacgg gcgcgatcat ggccgtgccc
    12121 ggacaggacc agcgcgactg ggacttcgcg atgaagttcg ggctgccgat cgtccgtacc
    12181 gtgcagccgt gcgacggctt cgacggtgag gcgtacaccg gcgagggccc agccatcaac
    12241 tcgtccaacg acgaggtgag cctggacggg ttgggcgtcg acgaggcgaa gtcgcgcatc
    12301 atcggctggc tggagtcgaa ggcgaacggc aagggaacgg tcaacttccg gctgcgggac
    12361 tggctgatca gccggcagcg gtactggggg gcgccgatcc cgatcatcca ctgcgcggtc
    12421 gacggcgagg tgccggtccc agcggaccag ctgcccgtcg tactgccgga gctgcacggt
    12481 ggcgagatgc agccgaaagg cgtgtctccg ctggcgtctg ccactgactg ggtcgatgtc
    12541 gaatgccccc gctgcggtgg ccccgccaaa cgcgacacgg acacgatgga cacgttcgtc
    12601 gactcgtcgt ggtatttctt gcgctacccg acgccgaacg acgccaccca gccgttcgac
    12661 gtcgaggcgg tccggcgttg gggtccggtc gaccagtacg tcggcggggt cgagcacgcg
    12721 atcctgcacc tgctgtacgc gcggttcgtc acgaaggcgc tgcacgacct gggctacctg
    12781 gacttcgtgg agccgttcac ggcgttgatg aaccagggtg aggtcatcaa ccagggcaag
    12841 ggcatgtcga agtcgctggg gaacggcgtc gacctggggg agcagatcga gaagttcggc
    12901 gtcgatgcga tccggctgac gctggtgttc gccggtccgc cggaggacgc catcgactgg
    12961 gccgacgtct caccgggtgg ttcgctgcgt ttcctgcagc gggcctggcg gctggcgggg
    13021 gatgtgacca gcgcacctgg caccgacccg acgggaggtg acgcggggtt gcgcaaggtc
    13081 acccacaagg tgatccacga cgcgacccag ctgctggaga tccaacgctt caacgtcgtg
    13141 gtcgcccggg tgatggagct ggtgaacgcc acgcgcaagg cgatcgactc gggcgtcggg
    13201 ccggccgacc cgtcggtgcg tgaggcagcc gagacggtgg cgatcatctt gagcctggtg
    13261 gcgccgtaca ccgccgagga gatgtgggag cggctgggat acccgccgtc gatcgcccgc
    13321 gtcggctggc cggtggcaga cgaggcgctg ctggtccagg acacggtgac ctgcgtcgta
    13381 caggtgcagg gcaaggtccg ggcccggctg gaggtgtcgc cggaggcctc cgagtccgag
    13441 ctggaacacc tggccaaggc ggacgagaac gtgttgcgtg ccgtgggcgg ggccgagatc
    13501 cgcaaggtca tcatccgtgc tcccaagctg gtcaacatcg tcaccggctg accggatacc
    13561 cgggcgtgac tgccgcagtt cgctgaccgc acaggcgcgt ccgtggtggt ttgcgatgtt
    13621 tacagcccaa aaggcgtgta aacatcgcaa accttcgatg ggcacagtgg gtttggggtg
    13681 cgaacatggc aaccaccagt ccgcgcagtc gcagatgtcc gaaaaccgcc agtcatatgg
    13741 ccgtcaaagc ggacggcgac ccgagcgccc ggcgcagcgg ggcgaacgtt ttcgcgtctc
    13801 gtcaccggct gaccggcata cccgggtgtg actgcagcag ttcgctgacc gacagctgcg
    13861 tcggtggtgg tttgcctcgg tagccatcca aacggacggg tagccaggca ggtctgcctt
    13921 gggagggatg ggtttggctg gttagccatg caaaccgcgg ccgcgaacga gcggatgtcc
    13981 gaaacggcca gtcagatcag gccacaagga acgggaagcc ggctggccag ctctgcgggc
    14041 accaacgtct cccgctcttc gctgccgacc cacctcagcc gggacgaccg aggatccggt
    14101 ggccacagcc accaaacctc gaccgtcgcc gctccacagg agccggagga ctcccagacc
    14161 tgccgtgcgg gcggtgagtc tgcctgggta gaggtccaga cgaacggtta gccaggcaga
    14221 cctgccttgg gaggggtggg tctgcctggc gaccgtggca gatcgggggg ctgaccgggc
    14281 ggatgtccga aacccgccag tcagacacac caggcaaaag gcacggaacg ccggccaaac
    14341 cggctcagca tgcacgaacg ctccccgctc ttcgctgcca acccaggtca gccgaagccg
    14401 acggagagcc ggtgaccggc ccagcgagcg cgaacgattc ccgcccctcg ttgcccaccc
    14461 acctcggccc acgcggacgg cgtggcggag aaaacgcgcc cacccatcgc aacggtctcc
    14521 gcctgccgaa gcccgatcaa tccccggacc tgccccgacc tcagggcagt cggaccgaca
    14581 tccgaacaag ccaccctgcc gacgagtcat ccgccagctg gcgccagagc gcacaacagg
    14641 ttacgagccg gtaatctcac cgcatgaccc gctcgatcgt gctcgtgtcg gactccacgt
    14701 cggtggagcc tgagatggca cgtcggctgg acgtcaccgt cgttccgctg caggtgatca
    14761 tcggcgcgac gtcgtacgac gaaggcgtcg acgcagggcc cgacgatgtc gccgccgcct
    14821 tgcaccggcg ccagccggtg tcgaccagcc ggccggcacc ggaggcgttc ctgcgggtat
    14881 acgagggcgc ggcgaagcag ggcgccgagg cggtcgtgtc gatccatctg tccggagagg
    14941 tttcgggcac gttcgagtcc gctcagatgg cagctcgggg agcatcgatt ccggtgcact
    15001 gcgtcgacag ccgccaactc gggatgggca caggattcgc tgtcgaaacc gccgccgccg
    15061 ccctggacga cggcatctcg gcggagaagg tggccgaggc ggcatgcgag cgggcgcgca
    15121 gtacaaaggc gctcttctac gtcgacacac tggagttcct gcgtcgcggc ggccgggtcg
    15181 gtgccgcaac ggccatcttc gggtcggcac tggcggtcaa gccgttgctg gcgatcgacg
    15241 acggcacgat cgtcccattg gagaaggtgc gtacgacgac gaaggcgatc agccgattgg
    15301 aagagctggc cttcgaggct gccggcgatg ccgtcgtcga catcgccgtg tcccacctcg
    15361 caagtgccga cagagcttgt gacctggcgg accggctccg tgcacaggtg ccgaacgccc
    15421 gcggtctcgt cgtgaaccag gtcggcgccg tcatcggtgc ccatgttggc ccgggactcc
    15481 tcgcggttgt cgtggcgcca cgctgacccg ctgcgctgac ctgctgggct gacccgcgga
    15541 gctgacctgc tgggctgacc cgcggagctg acctgctggg ctgacccgcg gcgctgaggc
    15601 atgccgctgg accgtcgcgt gtcctggatc ctcgaacggt ccgtgggatg tccacaaccc
    15661 ttgtcgctgg aggcattcat ccacaggccg tcaatcggga ctgccgtggt gagtccaccc
    15721 tctcctagcg tgttgaggca tgtggggacg acggacatca cggaccagcg acaccgcgtc
    15781 gacagcgcgg cgtcggctgg ccgcgatcgc tgctgagttc gatcgcagtc cagttcccga
    15841 ccagggcagc tcgccggcgc cgtcgactcc cgtgcgcccg acacccgcgc cgaccacggc
    15901 cgactctgaa ggcggtcggc atcgtggtcc gggcggggtg tgggaccggc ggcgttggcg
    15961 actgtccgtc caccacgtcg ctgtgctgac gttcggtgcc gcggttctgc tgtcgctcgc
    16021 cgcgtggtgg gcgctgcgag cggtgccagc ggaagagccg gttccgcagt ccgaccggct
    16081 gggtgttccg acgcacccgt ccgcggactc tccgagcccg ggcacgccat cggtttcgac
    16141 ccccgtcgat ggcgtgcccg ccccagccgt cacggcggcc gcagcgcctg ctctggtcgt
    16201 cgacgtgacc ggaaaggtac gccgaccggg catcgtcgag ctatcgcctg gatcgcgggt
    16261 cgttgatgcg ctggcggctg ccggtggcgc tcggccgggt gtggacacca caggtctcaa
    16321 cctggcccgg ctgctcgtcg acggcgagca gatcgtcgta ggtctggcga tcccttcgat
    16381 gagggacacc accgaggctc cggccgtatc cgcgccgtcg accgggcctg cattgggaag
    16441 catcaacgtc aacacggcgt cgcaggcgga gctggagacc cttcccgaca tcggtccggt
    16501 gacggcgcag gccatcgtgg actggcgccg cgagaacgga tcgttcgcga gtgtcgacca
    16561 gctcgtcgac gtctccggta tcggtgaggc gacgttgcaa gacatcgagc cgtacgtcca
    16621 tgtatgacgc gcgaccgcct cgacctccgg ctggcgctgc ccgcactcgc cgtctgggga
    16681 gccgcggcct ggggagtggg ctggaccgct gtccacgcag tgctcggggc gctgtccctg
    16741 ctcgccctgt cgggcctggt tgcgtggcgc ggtcgccacc gccatggagc tgaccgatcc
    16801 ggtcaggccg atcgggcggc acgacaggaa cgtcgcgata gatctgcggc gcgagcacag
    16861 accgcgctgg ctcccctgct cctggcggct gctgcactca ccgtgacagc cctgcacgcg
    16921 agttcgatac atgctgggcc ggtcgacgag cttgcgtttg catcgagccg tgtcgaggtc
    16981 ggtggggtgg ttgccagcga cccggagttc aggatcggga cgttcgcacc gtacgtgctc
    17041 gtccggctcc gcgtgcaact cgttgtcgcc accggctcgc aggcacaggt tcgcactccc
    17101 gtcctggtga tcgccgatga ctcctggcgc gacgtgcggc tcggcgacca catcgtcgca
    17161 tccgggcggt tgaagccggc ggagggacac gacctcgctg cggtcttgat ggcggacccc
    17221 gatccgcagg tggtcggcga ggcttcgtgg gtgcagcgca gcgtcacggc ggtccggaac
    17281 gggttggtcg aggcaacgac cccgctcccg agcgctgagc acgctctggt ggttgcgctg
    17341 gtcgacggcg acgactcgag tatgccggcg gacacggtcc gcgacttcca gacaaccggg
    17401 ctcacccacc tgctcgcggt gagcggttcg aacctgacct tggtgctctc cttcgtgctc
    17461 ggactggccc gcgccatggg cctccgcggt cgttggcgac tcgcactcgg cggtctcgcg
    17521 gtggtgttct tcgtcctcct cgctcggccc gagcccagcg tgcttcgcgc agcggcgatg
    17581 ggtgtggttg cgctggccgg cctgtccgtc ggtggtcgtc gccgtggcat gcggggactg
    17641 tgcatcgctg tggtctgcct cgtgctgatc caacctgagc ttgcccgttc catcggcttt
    17701 ctgctgtcga cgtcggcgac cgccggaatc ctcctgtggg ccccggcgtg gcgcgaccag
    17761 ttggcacgct ggatgccggc cgcgttggct gaggctgtcg cggttccgct cgcggcgcag
    17821 ctcgcgtgta cgccggccat cgccgccatc tcaggacagg tctcgaccgt cgctgtcatc
    17881 gcgaacatcg ctgctgcgcc ggcagtcggg cccgcgacgg tgatcggact ggttgccgga
    17941 cttgtggcgc tggtgagtga gccgctcgcg cacttggtcg gacgattggc cggcataccg
    18001 gcctggtgga tcgtgacggt gtcacggcac ggtgcggact tcgcaggttc cagcctcgcg
    18061 tggccggtcg gaccggtcgc catcgtcgcc ctcggcctgc tgtgcctgct cgtcgtcttg
    18121 gtgctgcccc ggatcctcgc cagtcctgcg acgtcgctcg cctgcagcgt gctgctcgtt
    18181 ctcgtcgtcg tccagccgct cggccggctc ggatggccgc cggcgggctg gctcctcgtc
    18241 atgtgtgacg tcggtcaggg cgacggcatg gtgctcaacg ccggcggcgg ggttgccgtg
    18301 ctcgttgaca cgggcccgga cccaaggacg atggatcact gtcttgaccg actcgacgtc
    18361 aagcggatcg acctggtcat actcacgcac ttccacgccg atcacgtcaa cgggttgccc
    18421 ggcgccctgg agggtcgtga cgtgtccgag atcgagacca cgtcgctgcg ggacccaccc
    18481 gaccgcgcag cagctgtgga cgcctgggcg gcggactcgg gagtcacggt gaccgaggca
    18541 acgctggggg agtcccgcac catcggcgag ctgacgtgga cggtggtcgg accggtccgt
    18601 acgccgtccg acggactctc cgacgacgga tccgggccga acaacgccag cctcgtgatc
    18661 cttctggaga cgcacaacca cagcttcctg ctcagcggcg acgcggaacc tgaggaggag
    18721 gacgacatcc tcgccggaca cgtggacctc ggcgtggacg tgttcaaggt cgcgcatcac
    18781 ggctccgcga accagagtcc cgacttcgtg ctgagcacgc atgcgaggct cgccctcgtc
    18841 tccgtcggtg cggacaacga ctacggtcac cccgcgcccg agacgctcgc actgctacgc
    18901 cggctcggag cgatcaccta ccggaccgat ctcgacggtg acatcgccgt ggtcgaccgg
    18961 gccggtcagc tggcggtcgt gaccgcgcac tgatcagccg gcgttggcgg cggcttcccg
    19021 gaccagacgc tcgcggactg cgtgctcgtc ggtccgagcg tcgtatgacc agaagtcgcg
    19081 cagccacgcc gccgtcgctg ccacaccgat cacgcacaga acgccgccgg tcgtgatggc
    19141 ggcccgtacg gaccaccggt cagcaacgag accggcgcgg gtttggccac caagcgggcc
    19201 gagggagtac gacagcatct cgatcccggc gagccgaccg cgcatcgagt ccgggatcgt
    19261 ctggtgccag atgacgtccc ggaagatgcc gctcaccatg tcggcagcgc cggcgacggt
    19321 gagccagaac agaatgaaca acaccgacga cgagacaccc accagggcga tcgcagcacc
    19381 ccaggcggcg gccgcgatga cgacggcgcg accgtggtgg tggactcggg aagtccagcc
    19441 ggacgtggcc gtggccagca agccccccac cgtgcccgcg gtgtacagca gccccagcac
    19501 gctgggcgtc ttcaagacgt cttgagcgaa cgccgggaac agcacggtcg gcatggccat
    19561 gagcatcgcg acgatgtcga tgatgtaggt gccgagcagg tcccgccggc gaaccgcata
    19621 gccgattccc tccatgatgc cgcgcactga aggcgggttg gacgtgtcga gtgcggggta
    19681 ggggcgaagc agcgcgaaga gtgccgttgc gaccagcagc ccggacacgt cgaccgcgaa
    19741 gcaccacggc acgccaaccc actggagcaa caccccaccg gccgccggtc caccgagcac
    19801 cccgatctgc atcccgaagg aggacagtga tacggcggcc gggagttggg cgtgcttgac
    19861 ggttcgcggg atgagcgcct cccacgaagg tcgctgcaac gcctgggcag cggacagcaa
    19921 cgcgccgatc gcgtacagcg gccagacctg cggcgacggc atgaaggcgt tgatcatcaa
    19981 gcacgacgtc agcaggatct gtgccatgcc ggttgagacc agcatgatcc gtctgtccat
    20041 gtggtcggcg agcgcgccgc cccacagacc gaagaccacc agcggtacca gctcgacgat
    20101 gccgagtgcg ccgacggcga agttcgatcc ggtcagttgg tagagctggt agggcacagc
    20161 gacgtacgac accatgccac cgagatagaa gactgtcccc gcccagaaca gcagccggaa
    20221 gtctcgggac tcgcgcaatg gtgtcgtgtc gacacgcact ctcgcgaggc gttccttcca
    20281 cgtcacgcgg agaatggtga cagaggcgtg caccggaggc gaatcctttg cggccggcct
    20341 gcgtcgtcgc acgcccgtgg catgctgggc gcaccatggc gaagcagtct tcggagatga
    20401 gtctcggaac ttccaccgac gcagtgctcg caacgacgac gctcatcacc ggcggcgacg
    20461 agttcctcgc cgaacgcgcc atcgccggcc tgaagacggt cgtacgggct gccgacgtcg
    20521 acgccgacgt ggccgagctc gaggcggctg ccctcggcgt cggtgcgttg gccgagatcg
    20581 ccagcccgtc cctcttcgct gccaaccggt gcgtggtcgt ccgacgaatc gaggagctcg
    20641 cggacgaagc agtcgaacct ctgctggcgt acgtcgcgga gccgtctccc gatgtcgcgc
    20701 tcgtcctgca ccacaccggc ggtgtcaagg gcaaggcgac cctcgacaag ctccgcaagg
    20761 ccggagcacg cgagatcgct gcccaggggc cgaagaggtg ggagctgccc acgtgggtcc
    20821 aacgtgagtt ccgcagtcac ggcaagaagg tcgagaaagc ggccgccgca gcgctcgtcg
    20881 acgccctcgg cgacgacctg cgttcactcg ccggcgccgc cagtcagctc gccgtcgact
    20941 gtccggacga cacgatcact gcacagacgg tccgggccta cttcggtgga cgggccgacg
    21001 tgaaaggatt cgcagtcgcc gacgcggcga tcgacggaaa gggcgcggag gcgatcgagc
    21061 agctgcgctg ggcgctcggt ggccgatccc ccgccccggt gctgatcacc agcgcgatgg
    21121 cggccggtct tcgctcgatc gcccgcttcc aggcggctcc ccgcgggctt cgtgaaggcg
    21181 acctggcgcg tgaggtcggc gtaccaccgt ggaagctgaa gtcgatcgcg gctcagtccc
    21241 gtggctggtc agcccgtggg ctggcggcgg cgatccaagc ggccgcgcag gcggacgccg
    21301 acgtcaaggg cgccggtggc gacccactct gggcgtgtga acggctggtc atctcggtga
    21361 tccgcgcccg cgccctgcga tagcggctct gcgtacgccg ggagggcagc gaccgatctg
    21421 agccggtcgg agccggaaat gcaacgacgg gtcgacccga tcccggtcaa cccgtcgaag
    21481 acggtatcgc cactggccgg cgccggtggc tcgtcagcgt cagctcagag cgacgcagcc
    21541 ttcttggcga tggccgactt cttgttggca gcctggttct tgtggatgac gccggcggac
    21601 gcggccttgt ccagcttgcg cgaggcagcc ttcgccagct cggtcgcctg ctccgtctcg
    21661 ccggcttcgg cggcctcgcg gaaccggcgg atcgacgtct tgagcgccga cttgaccgac
    21721 ttgttgcgct cgtgcgcagc gttgttctgc ttgatgcgct tgatctggga cttgatgttg
    21781 gccacggggc aatgcctctt gctgggttga gtggatcggt ggtgctgctc ttcgcgtggc
    21841 cgcaaacctg ctggtcagcg ggccgacacg cgaccgctta ggctaccagt cggccgctga
    21901 gccggccaaa tcgtcgtaca acgccgattc gcccgactcg ccgccccgcc ggacccagcg
    21961 cttgcgcgct cgagtcgagg gtggtgcgag gatgcaccgc atgacatcag gcgcccttgt
    22021 ggagcaggcg ctggaccgga tcgactccat cgacccgctg atcaacgccg tctgcacgct
    22081 caacccggcc gcgctggacg atgcggccgc cagggacgcc gaggaaggcg acggccgcag
    22141 cagaggaccc ctccacggcg taccgatcct cgtcaaggac aacatcgaca ccgcgggact
    22201 ggcgacgacg gcaggttcgt tggcgctggt cgacatcccg ccgcccgcga ccgacgccac
    22261 gctggtgcag cgcctccgcg acgccggcat ggtggtgatc gggaaggcga acctcagcga
    22321 gtgggcgaac ctacgggacg agcggtccac gtccggatgg agcgcgtacg gcggcctgac
    22381 gcgaaacccg tacggcctca accggtccgc cggcggatcc tcgtccggca gcggtgcagc
    22441 cgtggccgcg gggctcgtcc ggtacgccgt cgggaccgag acggacggtt ccatcacgtg
    22501 tccggcggcg ttcaacgggt gcgtcggcat caagccgacg gtgggtctgg tgccgaccga
    22561 cggtgtcgtc ccgatctcct cgtcccagga ctcgccaggc ccgttgaatg cgaccgtcgg
    22621 cgaggcggcg gcgctgctcg gtgtcctcac cggggaaggc ccggaccact acacccgacg
    22681 gctggacgag ggcctgcgcg gccagcggat cggtgttccc cgcaaggtct tctggggcta
    22741 cagctcgcaa gccgacgaga tcgccgagga ggccctggcc ctgctctcgg gcgccggtgc
    22801 cgtgatcgtc gacgagaccg atctcgactc gatggtcgac ttcggtttcg acgacgagct
    22861 cgcggtgctg cttgccgagt tcgaggtggg tctgggtgcc tacctgcgct cgcgcgaagg
    22921 cgatggaccg aagtcgatgg ccgatctggt cgcgttcaac cgcgagcacg ccgacaccga
    22981 gctcgcgtgg ttcggacagg agtacctcga gaaggccctg atcgcacccg ggccggactc
    23041 accggagtac gccgaggcgc gggccaagtg cctgaaggcc gggcgtgacg acgggatcga
    23101 cgccgtcttg cgtgagcacg agctcgacgc gctcgtcacg ccgtcgtacg cccctgccat
    23161 cccgatcgac ctcgtcaacg ccgagcggca ccacggctcg tgcacgcagc catcggcgat
    23221 ggccggatac ccgatcctga ccgtgcccgc aggcctgtct cagggactgc ccgtggcgat
    23281 ctccttctgg ggcgctgcgc gcagcgagcc ggcactgctg gcgatgggca gcgggtacga
    23341 gcgggcacgc gacgcagccg ccggtgaact ggcgagcccg accttcccgc agttcgtctg
    23401 agggcggacc atgctccgag cccttctcta cggtctgggc accgcaggcc cactggtcgc
    23461 cggcgccgcg gcaggcctgt ggtggaagct gcccgaccgg gtcctcgcgg ccttgatggc
    23521 gttcggagcc gggaccatga tcgccgcagt gtcgtccgag ctgttccagc ccgcgttcaa
    23581 cgagctcggc ggcggctggg ccagcattgc cctgctgggc ggtgcggccg tgtacgtggt
    23641 ggccgaccga ctgatcgagc tgcgcctcgg accggcatcg ctcggttggg cgctgatgct
    23701 cgggaccgtc ctcgacggcg ttcccgagaa caccgctctg ggcgtgtcgc tgaccgagac
    23761 cggcggagtg gtgctgctgg tggcgatcgc ggtcggcaac ctgccggagg cgatcagcgg
    23821 ctccgcccaa atgcgaaggc accacagtct caaccctggc cacatcgtgc ttctgtgggt
    23881 tgctaccggg gctgtcctcg tggcggtgac cgttgtgggc tacgcggcgt cggatgtcgt
    23941 gcgtcccgcc tcgatcgctg ccgtccaggc gatcgcggga ggcgccacca tcgctgtgct
    24001 ctcggactcg ttgattccgg aggcgtatcg cgaaggcggt tggtggatcg gcttggcgac
    24061 cgcattcggc ttcgtggtgg cgttctcgct gggtgcctag cgggaaacta gaaaagactt
    24121 ccgacgtggg acactaacgc tggtcgagcc gctcgatgag gccgagggcg tcgtacggcg
    24181 cccgcacctt cgcgtcgttg tcgaagtaga cgtagacgtc cagtccgtcg ccgaaccagc
    24241 cgcgaacccg ctcggcccat cgatccagcg cgtccggggt gtagccgctg acgtagagct
    24301 cgtcggctcc gtgcaaccgg acgtacacga agtcggaggt caccgcgtcg atcatcggcc
    24361 aacgcccggc cgtgtcggca acgacgatcg ccacatcgtg ctcgcgcagc aggtcaaggc
    24421 tccggtcggt gacgaagctc tcgtgtcgta cctcgagggc gtgcttgaca ggaatgtcca
    24481 cgctcgcggt ggtgaaggcg cggtcctcgg tgatgcgctc gtcatggtgt ttcgacagcc
    24541 gcgcggctgc cgacgtcgtc cgcggcagct gctcgaagaa cgcaacgagc cgggcctcgt
    24601 cgaatccgaa cgtcggtggc agctgccaca gcaccgggcc gagcttgcgg cccaacgcga
    24661 gtacgccgga cgcgaagaag ttcgccaacg ggctcacggg gtcggcgagc ctcttcatgt
    24721 gggtgatgaa ccgcggcccc ttgaccgcga acacgaactc gtcgggtgtc tcgtcatgcc
    24781 agcggccgaa gctcgacggt cgctgcaggg agtagaacgt cccgttgatc tcgatggacg
    24841 tcagtcgctc ggcggcgtac tccagctcac gcttctgggc gagtcctttc gggtagaacg
    24901 tgccgcgcca ggcggggtag acccacccgg agatcccgac gcacacgtgg gccatcgacg
    24961 ctcaggacgg tcgtgggaag gacgacccga ccacggcgtc ccaggcacgg tcgggaagca
    25021 gcgcatgctg gcccatgaac agccgggccg acggcgtgac cgggtagcgg gtgcgcggcc
    25081 gccggcttgt gaccgccttc tcgatcgcct tcgccacggc ctcgggcgca ccacccagcc
    25141 tgcccagcgg gctgtggtag atcccagcgg tacttcgggc gaccgctgcg ttgtactcgg
    25201 cgtacggccc gtcctcctgc gtcaccgact cgacagctgt gtcggcgaag ctgctgcgga
    25261 tgaaacccgg ctcgatgatg cacacgtcga cgccgaaacc tctgacctcg aaccgcaaca
    25321 cgtcggagat ggcctcgacg gcgtacttcg tcccgtggta gatcccgcct ccaggaaacg
    25381 tcagccggcc acccatcgag ctgacgttca cgatccggcc gcgaccggct cgacgcatgc
    25441 cggggaggac cagttggcac atccggacca gcccgaacac gttggtctcg aactggcggc
    25501 ggacctcgtc catcggcacg gtctcgacgg cgccactttg gctgtatccg gcgttgttga
    25561 ccagcacgcc gacagaaccg gtctgctcct cgacagcggc gacggcagcc gccatcgagt
    25621 cctcgtccgt cacgtcgagc gcgagcgtcc ggcagtcggc ctcccggagg tcgtcgatcg
    25681 tctccagccg acgggcagtc gcgtacacct ggaggtcgtc gcggcgcgcg agtcgcgttg
    25741 cggtcgcgcg tcctatcccg gtcgagcaac cggtgatgag cacggacgtc gtactggctg
    25801 ggggtttgac ggtgccgtcg gacatgcgtc acagcgttcc actgctgtcg atctggtcaa
    25861 gtcgagccct cgccaccggc gcggagcgag aggctcggtg ctggggtcga cgggtcggcg
    25921 ggacggcggc ggcccgccga gttcggcaga ctttcgacca tgacggaaac cggcgagggg
    25981 cgacaggtcg gcgtcgagga ggagctgatg ctcgtcgacc ccgagactgg ccgccttcgt
    26041 gcggtgtcgg accgggcgct cgccgctcac cgcgatgtgc gctccgacga agcagacgat
    26101 gaggcgttcg gtgcgagtgg tggtctcgcg caggaactct tcctcgtcca gatcgagacc
    26161 ggcacgcaac cgacgcgcga catcgccgag ctggtcgccg acgtacgccg atgccggcag
    26221 tccgcagcgg agtcggctcg ctcggcggga gccgaactcg tggcgacagc gacggccgtg
    26281 atcgggggtg aggatcggga ggtcacccgc aaaacacggt acgagcgcat cgtcgggacc
    26341 ttcggggaga tctcccggca gggatccgtg tgcggtatgc acgtgcacgt cgaggtgcgc
    26401 gacgcagcgg aggcggtcaa ggtgatcgac cgcctccgtc cttggctccc cgtactccgg
    26461 gccatgagcg tgaactcgcc gttctactac ggcgtcgaca ccggctacgc cagctggcgt
    26521 acgcagatct ggagccggtg gccgaccgcc gggccctgcg agccgtacgg ggacttgtcc
    26581 gggtatcggc aagccaccga cgccctgatc gcaagtgggg ccgcgctcga cagggggatg
    26641 ctgtacctcg acgcccgggt gtccgagcac tacccgacga tcgagatccg ggtgttcgac
    26701 gtcgtctcgg agcccacgga tgtcgggctt ctcgccgccc tgacccgggc actcgtggac
    26761 acccttgccg aacgctcgga cggaccggtc tggcggagcg acctgaccaa ggccgcgcac
    26821 tggcttgcct cgcgtgacgg cctctcacgt gacctggtcg agccgttctc ggcggctccc
    26881 gtgtcggctc gtcaggtgat cgagcgaatg atcgaccact gcggcgagtc gctgcgtcgg
    26941 gccggtgacc tggatcgtgt cagcgagggc ctcgacctcc tcctcgctcg cggtaccggc
    27001 gccacccaac agcgagcggc cgccgagcgc accggatcca tggacggggt cgtcgccgac
    27061 ctgcgcgaac gcttcacggc gtccttgtcg gagcctcgac aagcgaactg agcagcggcc
    27121 gagcggtcac cgcgcaacgt cagcgggatc catgtacagc acctcccaga ggtgaccgtc
    27181 gacgtccgcg aagctccatc cgtacatcgg tccgtcgacg atcttggctg ccgacggcgc
    27241 accgccggac tccagcgcac gcgcgaccag gcgatcgacc tcgtcgcgac tgtcagccga
    27301 gatcgccagg atcacctcgt tgtgtgtcgt gctgtcgaca acggacttgg tcgtgaacga
    27361 tgcgtagaag tcgcggggca gcagcatcac gaaggcgtcg tcgctgacca ccatgcaagc
    27421 cgcctgttcg tcggtgaact cggggttgaa ccggaagccg agcttgctga agaacgcgac
    27481 ggagtcggca aggtcgttga ccggcaggtt gacgaagatc ttccggcgcg catgcatcac
    27541 agcggtcacg gcggtccccc tcgcgatcgg ttcacgtggt ctctccccgt accgaccact
    27601 cggcgtcgcc gaactcatcg cgcccgatgt gcgatggcga ctgggcgact cctcaggttt
    27661 ctgtcggcgg ggacggtcgc gatgggccgt gattcccccg gcgcaccctc cgcgtgggtt
    27721 gaggtggatc ggcaatgaaa agcgggagac gttcgtgcct gctgagctcg tccgccggct
    27781 ttccgtccct tttgcgtggt gtgcgactgg cggtttcgga catccctcgg tcgccggtgg
    27841 cggtttgcat ggctacccag gcaacctcac cgctcccgag gcagacctgc ctcgctaccc
    27901 atccgtttgg atggctaccc aggcaaacca ccaccgacgc aggtccgatt ggtgcggaag
    27961 atggctttcc ggaggcggga gggccgcgcg ggtgcacggg caggacgcag tcctgcccgg
    28021 agaagcctgc accggtagct ggcgcggccg ggagaaacct gaggggtcga cgaaaacctc
    28081 ccgagccggc gatgagttcc ggccagcagc gtggtcggta cgagtgagac tccgctcagg
    28141 acgggagata tcgatgtttg ccgagtccaa ggccttcagc ggcttcgccg tcgacgacgt
    28201 cgacgcggcc aagcgtttct acggcgaggt cctcgggttg accgtcaacg ccgtcgacga
    28261 cgagggccag ctgctccagc tcgagctcgc gggaggcacc aacgtgttcg tgtacggcaa
    28321 gcccggacac gtcccagcga cgtacacgat cttgaacttc cccgtcgacg atgtcgagcg
    28381 tgcggtggat gcgctgaccg cgaacggcgt cgccatgctc cggtacgacg ggttcggcca
    28441 ggacgagaag ggcatcgctc gcggcaacgg gcctgacatc gcgtggttca gcgacccctc
    28501 cggcaacatc ctttccgtcc tgtcgacgtg agtcaccggc cccgtacagt gaaggcaccg
    28561 aaccgggagg ccgttccatg agccaggacc tgctcgccag caccgacgaa ccctccacgc
    28621 ccgacgacga ctttcccaga ctggtcgacc cgtatcgccg cgagctgctc gcacactgct
    28681 accggatgat ggggtcgatc cacgacgccg aggacctcgt ccaggagacc tatcttcgtg
    28741 catggcgcag ctatcacgcc ttcgagaacc ggtcgtcact gcggacctgg ctttaccgca
    28801 tcgccaccaa cgcgtgcctg acggcgctcg acggcaagtc ccggcgcccg ctgccgacgg
    28861 ggctcggtgc gccgagcagc agtccggacg agccgctggt cgagcaaggc gaggttccgt
    28921 ggctcgagcc ggtcccggac gtgatgctcg gcaacgaccc caacgaccca gcgtcgatcg
    28981 tgacctcccg ggagagcatc cggctcgcac tgatcgccgc tttgcagtac ctgccgccgc
    29041 ggcagcgtgc cgtcctcgta ctccgcgatg tgttgcggtg gaaggctgcc gaggtcgccg
    29101 acgcactcga gacgtcaacc gcggcggtga acagcatcct gcaacgggcc agggcccagc
    29161 tcgagcgtgc ggacatcacc gaggacagca tcgtcgagcc cgagtccgcc gagcagcgtg
    29221 agctgctcga ccggtgggtc gatgcgttct gggagaagga cgtggccgcg atcgtgcgta
    29281 tgttcagcga ggacgcggtg tgggagatgc cgccctacac cggctggtac cgcgggccgg
    29341 aggtcatcgg ccgtctcatc gacacgaagt gcccaggtga cgtgcacgac atgcggatgc
    29401 tccccacgcg tgccaacggc caacccgcct tcgccctcta catgcgtacg ccgtccggcg
    29461 aattcgagcc gttccatctg caggtgctca cgatcggtcc gaacggcgtc cgccacgtgg
    29521 cggctttctt cgaccacgcg ctcttcgagc ggttcggcct tcccgcccgg ctgcccgccg
    29581 aatcaagggc ctgatcggcg tacacatgcc gttcgacctg tctgcgggcc ggtcgggccg
    29641 gacgttgatg ccggagctcg acctgctcga gcgcgcggtt gcgtacacgc gcctgagcct
    29701 gcagctcgtc gcgccggacg tcctgcatgc gcccacacct tgcgaggagt gggacttggt
    29761 cacgttgctc gggcacatgg acgactcgct ggccgcgctg gcggatgccg cggacgtcgg
    29821 ctacgtcgcg attcgtccgc tacccaggcc ggatcccgcc aacgccgtga ccagcatcaa
    29881 ggcccgcgcc tgcgcgctgc tcgccgcctg gacggcaggc ggtggcgcgg acctggtatc
    29941 cgtcgcagga tgtccgctga gcgcacgcgt cctggtctcg acaggcgccc tcgagatcac
    30001 gacccacggg tgggacgtcg ctcgagcgtg tggcgtcgac cgccagattc ctggcgcact
    30061 ggcgcaggtg ctgctgcgga tgctgcccga cctcgtccac gactcggatc gggtgacgcg
    30121 gttcgcgcat gcggtggacg tccccccgtg gtggagtccg agcgatcggc tcgtcgcggc
    30181 actgggccgg cagccggtct ggtcggcgca ctcccggccg gtctgacgtg atggtccgct
    30241 gatcccgctc gtcgatctcg aggagatcaa ggagagcgag gagatcgagg agatcgagga
    30301 gagggtcact catcgccggt gtccgcctgc ggcttcgcca cactcgcaca cgattgcctc
    30361 ccggctcgac tgccgaacgg cacgccgccg ttcgggattc accatcgacg gcgtacacct
    30421 gccaggcatc ggttccccag gtcagcacgg aacgtcggca ggtgcggcag ccccggcggg
    30481 ggcggcagcc cgctggacat ggtgaggaac atgccgaccg acgccaagac ccgggtggcg
    30541 tcgtgctgtt cggactcgtc gaggccggcc cggtccagca ggtcgtcgaa ccgtcccgtg
    30601 tccgcccatt cgagcccgag cacgagcaca tcgccccacc acggaccctg ccgcgccatg
    30661 ccccagtcca gcagcacgac ctggtggtcg gtgcgacgga cgagcaggtt gtcgtgccgg
    30721 acgtcccagt ggcagagcgt gtcgccgtgg acgggtcggc cgtcgcgcac cattgccgtc
    30781 aggtcggcat agtcctggcg cgcccatgcc ggcagccgag cgaacagctg cgccggcggg
    30841 tcggccaaca gcatccgctg gtgcttctcg agaatctcgg ccaccgacac ggtgtcgagt
    30901 ccgtcgggca tcggcgtgag ctcgtccgcc tgctggcgaa ccgcggtgaa gacggcctcg
    30961 atctggcttt cgtcatccca gtcggggtgg tcgccgtcga tgtcctccag cagcatcgcc
    31021 acccagtgtc cgtcgtcgta cgtgcccagc agatcggccc ggtacggcgc cggaccgatg
    31081 cgagacaaga cgtcgatctc gtgacggaag tgcgtcgggg tgtcggggtt gatgtcggag
    31141 ctgaccgctt tgacgaaggc ccggccgccg tccgccacga ccaccgtcgc cgccaccgca
    31201 ggagacatcc cgccggttcg cgggatcgtc gtcgagacct gtccgccgag ctggtcctcg
    31261 atccagctgt gcagcggccc agggatgtcg tcgtacgccg tccgcacccc cgtgagccgc
    31321 tcgccgaccc cgtcagccat ggtcgtcgac acgggccgag atgccgatgc gttgctccag
    31381 ccaacgcctg gtgacgtctt cgtaccaggc ctggtggtct cgcagatgtg gggagccggc
    31441 aggcacgtcg ccctccatgc gggtcgtcag gtacagccac aggttcgcca gccacgcgtc
    31501 caccgacctc gggtcgagcg ccgcggtgag cggatggtcg cagagcgcgg tatcggtgtc
    31561 caggccgtct ccgtatgcgg cgatcaggac cgtgacgaga tccagccagg gcgccgcgag
    31621 catcggccag ttccagtcgc agatccagac gctgccgtcg tctccgagca cgatgttgtc
    31681 atcgcggagg tcgagatggg ccaccgctgt cccttgcgtg catgtgaccg actcgccggc
    31741 cagcccgatg atcgcgtcga gccagcggct ttcgccgtcc cgctccagga cccgtgcgat
    31801 ccacgcgggc agctggtcgc gatcgccgtc atagggttcc aggtcgagcc cctgcggagc
    31861 ccgggcgagt gccggagcaa cctgggcgag cttgtcggtg acgagtcgca gttcgcgcgg
    31921 ccgccacggc cgccgcggcg gatgaccatg gatgtacggg aagccgagca cgatccaggc
    31981 gtcgtcctcg tcgtcgacgt cgagcgacca cgacagcggg atcgcgccga cgtctgaggg
    32041 cagcagccga aggttgcggg cctcgatccg gtacgccgag ctgatctgcc agccgtgcag
    32101 gcggtcgtcg cgggtcgacg ccgccttgac gaagcatgcc gagccgtccg ccagatccag
    32161 ccgggatgcg aagccggccg tgaaccccgt ccccgtcgtg gtcgcggtga cgacggggga
    32221 gcccgcggcg tactcgatgg ctgtctggac cacggccgga aggccctgcc aggttcgccg
    32281 ccgcgcggtg gcccgaaccg gcaccaccac cccggtcggg agcatgacgc tgtcgttctg
    32341 ccattggtgg tcgtcgatgg gcgctgcgac ggccggagca gcggcagggt agccacgggt
    32401 ggcgtcagac cggccggcgt tcatgccacg agtgtcgccg aggtgcgcgt cctcgggcca
    32461 accgattatg gctgttcgag gttgcacgtc cggcggaccg gctgagactg ccgtcgacgg
    32521 gggcgactca gcggagccgg tcgagccgac cctgccgagg acggctcccg gcagtagctg
    32581 cccgatggcg cccccacggc gatctgccgc ggacgcgggt ccggcccgtg ggagaattgc
    32641 agaactgtgc gaccggtcta gccctgtgat cgggtgcgcg catctgttca cgacttcgac
    32701 ccggaagaac gacctgcccg tggccaaccc gtccaagacg atcgcgtccc cgccgcagcc
    32761 ggggcggacc gccccgtcga tcatccgtaa cttctgcatc atcgcccaca tcgaccacgg
    32821 caagtcgacg ttggcggacc ggatgctcca gctgacgggc gtggtggacg cgcgcagcat
    32881 gcgggctcag tacctcgacc ggatggacat cgagcgcgag cgcgggatca ccatcaagag
    32941 ccaggccgtc cggttgccgt ggacggtgtc cgacggcgac gcggcgggta cgacgtacgt
    33001 gctcaacatg atcgacaccc ccggccacgt cgacttcacc tacgaggtga gccggtctct
    33061 cgcggcgtgc gaaggtgccg tgctgctcgt cgatgccgcc cagggcatcg aggcgcagac
    33121 cctcgccaac ctgtacctcg cacttgatgc agatctcgac atcatcccgg tgctgaacaa
    33181 gatcgacctc cccagcgcac agccggagaa gtacgccgcc gagctcgcgc acatcatcgg
    33241 ctgcgacgcc gacgacgtac tccgcgtgtc ggcgaagacc ggtgcgggcg tgaccgaact
    33301 gctcgacaag atcgttgcca gcgtgcccgc gccacggggg gttgcggagg cgccgccgcg
    33361 ggcgctcatc ttcgactcgg tctacgacac ctaccgcggc gtcgtgacgt acgtgcgcgt
    33421 cgtcgacggc aagctcagtc accgggaccg gatcaagatg atgtcgaccg gagccgtgca
    33481 cgagatgctc gaggtcggc
//
LOCUS       DATBFZ010000565         7369 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_3929131, whole genome shotgun sequence.
ACCESSION   DATBFZ010000565 DATBFZ010000000
VERSION     DATBFZ010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 7369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7369
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3929131"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..599
                     /locus_tag="VH419_17340"
     CDS             <1..599
                     /locus_tag="VH419_17340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020387825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY7045439.1"
                     /translation="TPGCLRSATFYPEPGAAAYVARRIGAGAEIIKVHVQVGGFDVRD
                     PLLADVWGVLEDAGTPVVIHAGSGPVGTPYTGPGPVAELLASHPRLALVIAHLGAPEY
                     AEFLDLVAAYDHVRADTTMVFTPFFDRLGAAFPPDLLPRLRDLQPKILLGSDFPNIPY
                     QYAEQLAALARLDLGDDWLRDVCWHNGAELFGGVSPTM"
     gene            770..1140
                     /gene="ssrA"
                     /locus_tag="VH419_17345"
     tmRNA           770..1140
                     /gene="ssrA"
                     /locus_tag="VH419_17345"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1147..1569)
                     /locus_tag="VH419_17350"
     CDS             complement(1147..1569)
                     /locus_tag="VH419_17350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020574201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HEY7045440.1"
                     /translation="MPKPPLPDRVRDLLAKANPSVITTVRPDGQPVSVATWYLWEDGR
                     LLVNMDEGRTRLAYLRHDPRVTLTILDNDTWYTHVSVQGRVVEIRDDTGLADADRLAR
                     RYLGVPYPKRERRRVSAWIEVERWHGWGDVENTDVIHH"
     gene            complement(1586..2665)
                     /locus_tag="VH419_17355"
     CDS             complement(1586..2665)
                     /locus_tag="VH419_17355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013861879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEY7045441.1"
                     /translation="MPQTRDQAPIGTNDDRIPRDVPSVSPLPLMQLSIGFWSFKALAT
                     AYELDLFARLSGTDGRTAEELAEMLGVGRRPAELLATACASIGLLERRDGRYLNSAMA
                     DEFLVPGKTHHFGGWVQMLDRRLYPAWGRLTEAMRTGRPTSWNPDEQAHLFDSEDPQL
                     LALFWEAMHSLSTLTARELGAHVDLSGSTALLDVGGGSGAFDIELCRRHPDLRATVLD
                     LPPVCEIAEEKIEASGYADRIAVTPGDFLADRELPAGHDVVLLSMILHDWTPEQNLAI
                     VHQCFAALPPGGLIVISELLVNDEKTGPPAAALMSLNMLVETVGRNYTAAEYEQWLDA
                     TGFVDIRTVHFEAPGANGAVLARKL"
     gene            2785..3663
                     /locus_tag="VH419_17360"
     CDS             2785..3663
                     /locus_tag="VH419_17360"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY7045442.1"
                     /translation="MLERHELEAFLTLAEELHFGRTAERTHVSTARVSQTIKKLERRV
                     GAPLFNRTSRRVELTPIGQRLYDDLRPAREMMETAFERAVRSGRGITGTLQVGFVGAA
                     GGQLLSEAAELLHARRPGCQVLVREVQLVDAVPWLLSGEVELLLTCFDSARDDLAAGP
                     ALVTEARMLAVPAGHPLAERTSVSVEDLADVTVVRTPDDLPDSLHPPEAAGLLEPVPG
                     AGSFQEALTLVGAGRGVLPIGAHIRRYYTRPDVAYVVLRDAPPVHWRLMWRSDGATAR
                     VRAFAEVAHDLVSAAA"
     gene            3724..3876
                     /locus_tag="VH419_17365"
     CDS             3724..3876
                     /locus_tag="VH419_17365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7045443.1"
                     /translation="MTRLDDYIALRQVAFVLWFTGMASVNPAFASDLDKVRRWSLTLL
                     DLVERQ"
     gene            3905..4489
                     /locus_tag="VH419_17370"
     CDS             3905..4489
                     /locus_tag="VH419_17370"
                     /inference="COORDINATES: protein motif:HMM:NF025343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY7045444.1"
                     /translation="MSPARGPVEGRKDRRSLILDAVLTLLGREGMAGVTMRAVAREAG
                     VALGLVHYYFDDKPGLISAALRRVEEQDLLIVEPDTSLAPDRRLRAALRRVADPEFLT
                     TEYLSLRMQLWALARVNDDFAEINSSAQRRYRAALAALIRAARPALTRAECARRAADI
                     DVLQNGVWLTALLGLDRSSIRRSVARTEEIALAD"
     gene            4556..5728
                     /locus_tag="VH419_17375"
     CDS             4556..5728
                     /locus_tag="VH419_17375"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-hydroxylating oxygenase subunit alpha"
                     /protein_id="HEY7045445.1"
                     /translation="MAPVTADEVRAIAEGYDADPARSFSLRSAAYTEPRWAEADMAAI
                     FARTWQWVCHTEKLAEPGSYVATTIAEMPIAVVRDRAGRLRAFYNVCRHRAHELLAGS
                     GTTRNIVCPYHAWTYALDGQLQGARRADRMATFEKSEICLRQVRVEEFGGFIYVNLDQ
                     TARSLGEQAADLAADIERWAPDVAGLTHAKRLTYDVRTNWKNVVDNFLECYHCHIAHK
                     EFVDLVDMDTYEVRTHGIWSSHFAEAGKHANAAYDVSQARITQHAVWWLWPNTCLLRY
                     PGRSNFMVFQVLPTGPDTTHETWDFFLETTELDDAERQSVRYIDEVLQQQDIALVESV
                     QRGMSTPAFDQGRLVCDPEGSGLSEHGVHHFHGLVLDAYRCLVGLEPAADRESAGA"
     gene            5730..6656
                     /locus_tag="VH419_17380"
     CDS             5730..6656
                     /locus_tag="VH419_17380"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEY7045446.1"
                     /translation="MREPVRVAVLGGGLIGRSWTALFLAGGMPVCVHDPDPAAEARVR
                     DAVEHAWPVLTELGLADGDVRSDALTFSRDAASAVAGAGFVQESIPERLALKHRLYAE
                     IEPVLDDDAVVASSASGLTLSELQAGWASPGRLVLGHPFNPPHLIPLVEVMGNEHTDA
                     GVVESARRFYESVGKVTIEVRREVPGHVANRLQAALWREAIHLIDTGVASVRDVDVAV
                     SSGPGLRWALMGPTTLFHLGGDEGGIAAFCERYADSFHRWWDDLGDPRLDAATVHRLV
                     EGMSTVASVDELAARRDAALTAVIAATHPRSG"
     gene            6762..6941
                     /locus_tag="VH419_17385"
     CDS             6762..6941
                     /locus_tag="VH419_17385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7045447.1"
                     /translation="MPIVFIVIAGVVLLALAAAGVWMLAPSLRIFRHLDFDPPASSED
                     AVFFAENMKIVDGGQ"
     gene            complement(6949..7194)
                     /locus_tag="VH419_17390"
     CDS             complement(6949..7194)
                     /locus_tag="VH419_17390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY7045448.1"
                     /translation="MTSDGDAFQVMAPDHAYAALFGQPAGSPVPLFEVDDLAEARRDL
                     DSAGVEIIGPIDRDDRWEWLTFRAPDGHLYELAARRR"
BASE COUNT         1087 a         2574 c         2541 g         1167 t
ORIGIN      
        1 ggacgccggg gtgcctccgc tccgccacgt tctaccccga gcccggtgcg gcggcgtatg
       61 tcgcgcggcg gatcggggcg ggggccgaga tcatcaaggt gcacgtccag gtcggcggct
      121 tcgacgtgcg tgacccgctg ctggcggacg tgtggggcgt gctcgaggac gccggcacac
      181 cggtcgtgat ccacgccggc tccggccccg tcggcacgcc gtacaccggg cccggaccgg
      241 tcgccgagct gctcgcgtcg cacccccgcc tggcgctggt gatcgcgcac ctgggcgccc
      301 cggagtacgc cgagttcctc gacctcgtgg cggcgtacga ccatgtccgc gcggacacga
      361 ccatggtctt cacgccgttc ttcgaccggc tcggcgcggc cttcccaccc gacctgctgc
      421 ctcggctgcg cgacctgcag ccgaagatcc tgctcggctc ggacttcccg aacatcccct
      481 accagtacgc cgagcagctg gccgctctgg cccgtctcga cctcggcgac gactggctgc
      541 gcgacgtgtg ctggcacaac ggcgccgagc tgttcggggg cgtgtcaccg accatgtgag
      601 cgcgacacgc cgcgcccagg tacggcgtgt cgcaccgaaa cggtcggtga tacgcaagcg
      661 cggccgcttg tcggggaatc gagttcgtga cctgggcgtt ctcacccgta gaatggagta
      721 cgtcgtcggt cgagcgtgtc gagaccacga cgtgagtcac aactcaagag gggctgatcg
      781 gtttcgactt gggacgtcag ttccaggaga agcgggtcga gaagccggcg tcatctcgta
      841 aacgttcgcc ggaaaccaat aagtgccaac gctaagcgca ctgacttcgc tctcgccgcc
      901 tgagcggtga ccgaagggtc tgaccgggtg actgccttcg acccggaccc aggcctcatc
      961 tagaaggctt gctggccgct ctctgtcagg aggggcggcc gggacttcat cctgactgag
     1021 tccgtcggtg gacgtgtcag tgcgagcacc gggactgaga aaacgccatc actgactgca
     1081 cccggagaag acctggttcg acgctcgagg acgcgggttc gattcccgcc agctccacca
     1141 accgcctcag tggtggatga cgtcggtgtt ctcgacgtct ccccatccgt gccagcgctc
     1201 cacctcgatc caggcgctga ctctgcggcg ctcgcgcttc gggtacggca cgccgaggta
     1261 tcgccgggcc aaccggtcag cgtcggccag ccccgtgtcg tcgcggatct cgacgacccg
     1321 tccctggacg ctcacgtggg tgtaccacgt gtcgttgtcg agaatcgtca gcgtgacccg
     1381 tggatcgtgg cgcaggtacg cgagcctggt gcgaccctcg tccatgttga cgaggaggcg
     1441 accgtcctcc cagaggtacc acgtggcgac ggagaccggc tgaccgtccg gccgtaccgt
     1501 cgtgatgacg gacgggttgg ccttggcgag caggtcacgg acccgatcgg gaagaggtgg
     1561 cttcggcacg tgactccccg gttcctcaca gcttgcgggc gaggacggcg ccgttcgcgc
     1621 ccggagcctc gaagtgaacc gtccgaatgt ccacgaaccc ggtggcgtcc aaccactgct
     1681 cgtactccgc ggcggtgtag ttgcggccca ccgtctccac cagcatgttc aggctcatca
     1741 gggcggcggc gggtggcccg gtcttctcgt cgttgaccag cagctcgctg atcacgatca
     1801 ggccgccggg cggcagggcc gcgaagcact ggtgcacgat cgccaggttc tgctcgggtg
     1861 tccagtcgtg caggatcatg gacagcagca ccacgtcgtg gccggcaggg agctcgcgat
     1921 cggccaggaa gtctcccggg gtgaccgcga tccggtccgc gtagcccgag gcctcgatct
     1981 tctcttcagc gatctcacag accggcggca ggtcgagcac cgtcgcccgc aggtcggggt
     2041 ggcgccggca cagctcgatg tcgaaggcgc ccgagccgcc gccgacgtcg agcagggccg
     2101 tggagccgga caggtcgacg tgtgcgccga gctcgcgtgc ggtgagcgtc gacagtgagt
     2161 gcatggcctc ccagaacaga gcaagtagct gagggtcctc gctgtcgaac aggtgagcct
     2221 gctcgtcggg gttccagctg gtcggccggc cggtgcgcat cgcctcggtc agcctgcccc
     2281 aagccgggta cagcctgcgg tcgagcatct gcacccagcc gccgaagtga tgtgtcttgc
     2341 cgggcaccag gaactcgtcg gccatggcgc tgttgaggta tcgtccgtcc cggcgctcga
     2401 gcaacccgat ggacgcgcag gccgtcgcca gcagctcggc gggccgccgg ccgacgccca
     2461 gcatctcggc gagctcctcg gcggtgcgac cgtcggtgcc ggacagccgg gcgaagaggt
     2521 cgagctcgta ggcggtggcg agcgccttga aggaccagaa gccgatggag agctgcatca
     2581 gcggcaacgg cgagacactc ggcacgtcac gggggatgcg gtcgtcgttc gtcccaatcg
     2641 gggcctggtc acgtgtttga ggcatgaact ctccttgctc acgtcgtgcg aagctcgagc
     2701 tcccaccgtc gtccgagccc ccgggtcgat caacacggac ctgtgccaca ctcgtgaagc
     2761 gtggcttaac gatgggagtg tggagtgctg gagcgccatg agctggaggc gttcctgacg
     2821 ctggccgagg agctgcactt cggccgcacc gccgagcgca cccacgtgtc caccgcccgc
     2881 gtcagccaga cgatcaagaa gctggagcga cgggtcggtg cgccgttgtt caaccgcacc
     2941 agtcgccgcg tcgagctgac cccgatcgga cagcggctgt acgacgacct gcggcccgcg
     3001 cgggaaatga tggagaccgc gttcgaacgc gcggtcaggt cggggcgggg catcaccggc
     3061 accctgcagg tcggcttcgt cggtgcggcc ggaggccagc tgctgtcgga ggccgccgag
     3121 ctgctgcacg ctcgccggcc cggctgccag gtcctggttc gcgaggtgca gctcgtcgat
     3181 gccgtgccat ggctgctcag cggcgaggtg gagctgctcc tgacctgctt cgacagtgcg
     3241 cgggacgacc tcgctgccgg accggcgttg gtcaccgagg ctcggatgct cgccgtgccc
     3301 gccggacatc ctctggccga gcggacgtcg gtctcggtcg aggacctcgc cgacgtcacc
     3361 gtggtgcgga cgcccgatga cctgcccgac tccctgcacc cgcccgaggc tgcgggactg
     3421 ctggagcccg ttcccggggc cgggtccttc caggaggcgt tgaccctcgt cggtgcgggt
     3481 cggggcgtct tgccgatcgg cgcgcacatc aggcgctact acacgcgccc ggatgtcgcc
     3541 tacgtcgtcc tccgagatgc gccgccggtg cactggcggc tgatgtggcg gtcggacggt
     3601 gccaccgccc gggtccgggc cttcgccgag gtggcacatg acctggtgag cgccgccgcg
     3661 tgagcgcggg gcgccaggag ccgatgagat cccggcgcgc ccagatcgca ccgcgacgtc
     3721 gacgtgaccc gtctggacga ctacatcgcg ctccgccagg tcgccttcgt cctctggttc
     3781 accggcatgg ccagcgtcaa cccggcgttc gcatccgacc tggacaaggt tcgccgctgg
     3841 tcattgaccc tgctcgacct cgtcgagcgg cagtagcggc cctcgcacgc tctaccctga
     3901 cccggtgagt cccgcgcgag gccctgtcga ggggaggaag gaccggaggt cgctcatcct
     3961 ggacgcggtg ctcacgctgc tcggccgaga ggggatggcc ggcgtgacca tgcgcgcggt
     4021 cgcgcgggaa gccggtgtcg cgctcgggct cgtccactac tacttcgacg acaagcccgg
     4081 cctgatcagt gctgcgttgc gtcgcgtcga ggagcaggac ctgctcatcg tcgagcccga
     4141 cacatcgctt gcccccgatc gccgtctgcg cgccgccctg cgccgggtcg ccgatccgga
     4201 gttcctgacc accgagtacc tctcgttgcg catgcagctg tgggcgctgg cgcgggtgaa
     4261 cgacgacttc gcggagatca acagcagtgc gcagcggcgc taccgggccg cgctcgccgc
     4321 gctcattcgt gccgcgcggc ctgcgctgac ccgcgccgag tgcgctcgac gtgccgcgga
     4381 catcgacgta ctccagaacg gcgtctggct caccgcactc ctcgggctcg accgctcctc
     4441 gatccgtcga agcgtcgccc ggaccgagga gatagcgctg gcggactgac gggggatgct
     4501 ggacgttcgt ccagaaatat tgaacgaacg tccaatgcag cgatagcctc ccgtcatggc
     4561 acctgtcacc gccgacgagg tccgcgccat cgccgaaggg tacgacgcgg atccggcccg
     4621 gtccttctct ctgcggtccg ccgcctacac cgagccgcgg tgggccgagg ccgacatggc
     4681 ggcgatcttc gcgcggacct ggcagtgggt gtgccacacc gagaagctcg ccgagcccgg
     4741 aagctatgtc gctacgacga tcgccgagat gccgatcgcc gtggtccgtg accgagccgg
     4801 ccgcctgcgg gcgttctaca acgtctgccg gcaccgcgcg cacgagctgc tggccggctc
     4861 gggcaccacg cgcaacatcg tgtgtcccta ccacgcgtgg acctacgcac tcgacgggca
     4921 gctccaggga gctcgtcgcg cggaccggat ggcgacgttc gagaagagcg agatctgcct
     4981 tcgccaggtc cgcgtcgagg agttcggcgg gttcatctac gtcaacctcg accagaccgc
     5041 ccgatccctc ggggaacagg cggcggacct cgccgccgac atcgaacggt gggcgcccga
     5101 cgtcgccggg ctgacccacg ccaagcgcct cacgtacgac gtgagaacca actggaagaa
     5161 cgtcgtcgac aacttcctgg agtgctacca ctgccacatc gcccacaagg agttcgtcga
     5221 cctcgtcgac atggacacct acgaggtccg gacgcacggc atctggtcga gccacttcgc
     5281 cgaggcgggc aagcacgcga acgcggcgta cgacgtctcg caggcgcgta tcacccagca
     5341 cgccgtctgg tggctctggc cgaacacctg cttgctgcgc tatcccggcc gcagcaactt
     5401 catggtgttc caggtcctgc ccaccgggcc cgacaccacc cacgagacct gggacttctt
     5461 cctcgagacg accgagctcg acgacgcgga gcggcagtcg gtccgctaca tcgacgaggt
     5521 gctgcagcag caggacatcg ccctcgtcga gagcgtccag cgcggcatga gcacccccgc
     5581 cttcgaccag ggccgcctcg tctgcgaccc ggagggctcc ggtctgtccg agcacggcgt
     5641 ccaccacttc cacgggctcg tcctggatgc ctatcggtgc ctggtcggcc tcgagccggc
     5701 cgccgaccgt gagagcgcag gagcctgacg tgcgcgagcc ggtccgggtc gccgtgctgg
     5761 gcggtggcct catcgggcgc agctggacgg ccctgttcct agcgggcggc atgccggtct
     5821 gtgtccacga ccccgacccc gccgccgagg cgcgggtccg cgatgcggtg gagcacgcgt
     5881 ggccggtgct gaccgagctg ggactggcgg acggcgacgt ccgctccgac gccctgacct
     5941 tcagccgtga cgcagcgtcg gccgtggcag gcgccgggtt cgtgcaggag agcatcccgg
     6001 agcggctggc cctcaagcac aggctgtacg ccgagatcga gccggtcctc gacgacgacg
     6061 ccgtggttgc ctcgtccgcc tcgggtctga cgctgtcgga gctccaggcc ggttgggcca
     6121 gtcccggccg cctcgtcctc ggccatccgt tcaacccgcc gcacctgatc ccgctggtcg
     6181 aggtgatggg caacgagcac accgacgccg gcgtcgtcga gtcggcgcgc cgtttctacg
     6241 agtcggtcgg caaggtgacg atcgaggtac gtcgcgaggt gcccggtcac gtggccaacc
     6301 ggctccaggc ggccctctgg cgcgaggcca tccatctgat cgacacgggg gtcgcctctg
     6361 tccgtgacgt cgacgtcgcc gtctcgtccg ggccgggcct gcgctgggcg ctgatgggcc
     6421 ccacgacgtt gttccacctc ggtggtgacg agggcggtat cgcggccttc tgtgagcggt
     6481 acgccgacag cttccaccgc tggtgggacg acctcggcga tccccgcctc gacgcggcga
     6541 ccgtgcaccg tctggtcgag gggatgtcca cggtcgcgag cgtcgacgag ctcgcggccc
     6601 ggcgggacgc cgcactcacc gccgtcatcg ccgccacaca cccgcgatcg gggtagccgc
     6661 cgcatacggg tgacgtggag ctctccgcgg ctgcgctccg atccgtcgct ggcgggggaa
     6721 aagcggagta tggtttcttc acgtcaagag aggtggtcct catgccgatc gtgttcatcg
     6781 tgatcgcagg ggtcgtcctg ctggcactcg ctgccgcggg cgtgtggatg ctcgcgccgt
     6841 cgctacggat cttccgccac ctcgacttcg atccacccgc ctccagcgag gacgccgtgt
     6901 tcttcgcgga gaacatgaag atcgtcgacg gcggccagtg acgccgcctc acctgcgtcg
     6961 agcggccagc tcgtagagat gcccgtccgg cgcacggaac gtgagccact cccagcggtc
     7021 gtcgcgatcg atgggcccga tgatctcgac acccgctgag tcgaggtcgc ggcgcgcctc
     7081 ggccaggtcg tccacctcga acagcgggac cgggctgccc gcaggctgcc cgaacagggc
     7141 ggcgtacgca tggtcgggag ccatcacctg gaaggcatcg ccgtcggacg tcacgaactc
     7201 ggtggtcgcg ggttctcgaa gccgaccgcg aggccgaaga cgtcgcgaca gaacgccgtc
     7261 atctcggcgt agtgctgcgt tgcgacgccg acccagcgga gtgctcgtac gtgcatgtcc
     7321 ccaccgtcgc acccggcggg gacggtcggc ccggcgtcga cggtggccc
//
LOCUS       DATBFZ010000566         2417 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_5831888, whole genome shotgun sequence.
ACCESSION   DATBFZ010000566 DATBFZ010000000
VERSION     DATBFZ010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5831888"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..903
                     /locus_tag="VH419_17395"
     CDS             <1..903
                     /locus_tag="VH419_17395"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HEY7045449.1"
                     /translation="RYAKLPARRNEAASNVRAASELAHEKLVDAVVAGDASLGESRMV
                     KHLAAMRDWMLSTRQRPIGRSPGFLTMGLDGKDKLAEITARRITADIIRSHAKDGEVV
                     GSEAQLLQRYDVSRAVLREAARLLDYHSVARMRRGPGGGLVVSTPDVNASVQAAAVYL
                     DYRGVALNDLKAVRNVLELGGLELVMTRQDDPEVSNKLADRHQVRPDTPLAVIDDLSR
                     ELHRAIAELSGNPVLELFVALLTEVWARHHHSPADISTSDTGAAAHVARVHDRIVEAV
                     VAGDLPLARHRMHRHLDALSAWWE"
     gene            1301..1789
                     /locus_tag="VH419_17400"
     CDS             1301..1789
                     /locus_tag="VH419_17400"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY7045450.1"
                     /translation="MYGIELDALGARFDHVGVAVPRIADAIPIYMTLLGGRFLLANIE
                     ENDGFAALQLSFGHDGKFEIIQPYGPASPLARFLAAYPSGALHHVTFKVDNVRSSITA
                     AQNMGLEVVGIDLERSFWQQAYLHPRNTLGVLIQIVQSDTPFPRDPDLTLDRLLERLD
                     ES"
     gene            complement(1857..>2417)
                     /locus_tag="VH419_17405"
     CDS             complement(1857..>2417)
                     /locus_tag="VH419_17405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009077172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEY7045451.1"
                     /translation="AHDSDGPLTEEVFWSPIHNRVWKPVIVAVNGLVAGGGLHFVVDA
                     DIVVAAESAAFVDTHVNVGMVGAIENIGLAKRLPLGTALRMTLQGRNFRLPARRAYEL
                     GLVDELVAPEDLLATADTIAADIVGNSPHATSLSKQAIWAAQEMGYHQAMEYGWALAR
                     MHWSHPDLAEGARAFAEKRAPQWTTG"
BASE COUNT          418 a          824 c          787 g          388 t
ORIGIN      
        1 cgatacgcca agctcccggc gcgacggaat gaggctgcgt caaatgtccg ggcggcctcg
       61 gagctagcgc acgagaagct cgtcgacgct gtggtcgccg gggacgccag cctcggagag
      121 tcgcgcatgg tgaagcatct cgcggccatg cgagattgga tgttgtcgac ccgacagcga
      181 cccatcggtc gctcacctgg cttcctcacc atggggttgg acggcaagga caagctcgcg
      241 gagatcactg cacgccggat caccgccgac atcatcaggt cgcacgcgaa ggacggggag
      301 gtcgtcggct cagaagcgca actcctccaa cgatatgacg tgagccgtgc cgtacttcgg
      361 gaggctgcac ggctgctcga ttaccactcg gtggcgcgga tgcgccgcgg accaggcggt
      421 ggactggttg tctcgacccc ggacgtcaac gcctctgtgc aggccgcggc cgtgtacctg
      481 gactaccgcg gcgttgcctt gaacgatctc aaggccgtgc gcaacgtcct cgaactcggt
      541 ggtctcgagc tcgtgatgac gcgccaggac gatcctgaag tctcgaacaa gctggccgac
      601 cgccatcagg ttcgaccgga taccccgctg gccgttatcg acgacttgtc ccgcgagttg
      661 caccgtgcga tcgccgaact gtcgggcaat ccggtgctgg aactcttcgt cgccctgctg
      721 acggaggtgt gggcccgtca ccaccacagc ccggccgaca tctccacctc ggacacaggg
      781 gccgccgcac acgtagcccg cgtgcacgac cgcatcgtcg aagccgtcgt ggcaggcgac
      841 ctgcccctgg cgcgtcaccg gatgcaccgc cacctcgacg cgctcagcgc ctggtgggag
      901 tgaggttcga caccgcgcgt tagcgcgaat ctggaagagc gcccacccgg cgcggccgct
      961 caaacgtggg agccacgtcg gcaggtttgc gcaggtgacg ctttgccgaa ctgcgggtgg
     1021 gcgctgtccg ccccgtcgag acggcagaag gcgcgctggc gacgccctac gacgcggtag
     1081 cgggccgtgt cgcgctgacg gccgaccgtc ggcgctacct catgcccgcc ggcagcaggg
     1141 gttgtcggca ggttcggcaa tgggtgggtg atctccgcga cgtgacccga ccgcgctgag
     1201 cttgcacgca cggcatcgct actgggtaca ctcagattgc agactagtgt cgagtcacca
     1261 gcgtggaggg gttgtcgtgg accacacggc cctacaatca atgtacggta ttgaactcga
     1321 cgccctgggg gcgcgcttcg atcacgtggg ggtggcggta ccccgcatcg ccgacgcgat
     1381 accgatctac atgaccttgc tcggcggccg gttcctgctg gccaacatcg aggagaacga
     1441 cggcttcgcc gccctccaac tctccttcgg ccacgacggc aagttcgaga tcatccagcc
     1501 ctacgggccc gcaagcccgc tcgcccgttt cctggccgca taccccagcg gagcgctgca
     1561 ccacgtgacc ttcaaggtcg acaacgtcag atcctcgatc accgccgccc agaacatggg
     1621 cctggaagta gtcggcatcg acctcgagcg ctccttctgg caacaggcct acctacaccc
     1681 gcgcaacacg ctcggagtcc tcattcagat cgtccagtcc gacacaccgt tcccccgaga
     1741 cccggacttg acactggacc gactgctgga acgactggac gagtcctgaa cccggcccac
     1801 gccctcaagc aggtcggtcg cgcggggtca ggaggacgag ccggagaact cctccttcat
     1861 ccggtggtcc actgcggcgc acgcttctcg gcgaatgcgc gggcgccctc ggccagatcg
     1921 gggtggctcc agtgcatgcg cgccaacgcc cagccgtact ccatcgcctg gtggtaaccc
     1981 atctcctgcg cggcccaaat cgcctgcttc gacagggacg tcgcgtgcgg ggagttgccg
     2041 acgatgtccg cggcgatcgt gtcggcggtg gcgaggaggt cctccggggc gaccagctcg
     2101 tcgacaaggc ccaattcata ggcccgacgt gcgggcagcc ggaagttgcg cccctggagc
     2161 gtcatacgca gggcggtgcc gaggggcagt cgcttggcca ggccgatgtt ctcgatggcc
     2221 ccgaccatcc cgacattgac gtgcgtgtcc acgaaagccg ctgattcagc ggctacgacg
     2281 atgtcggcat cgacgacgaa gtgcagaccg ccaccggcga cgaggccgtt gacggcgacg
     2341 atgacgggct tccagacgcg gttgtggatg ggcgaccaga acacctcctc cgtcaacggc
     2401 ccgtcggagt cgtgagc
//
LOCUS       DATBFZ010000567         2836 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_5834928, whole genome shotgun sequence.
ACCESSION   DATBFZ010000567 DATBFZ010000000
VERSION     DATBFZ010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2836
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5834928"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="VH419_17410"
     CDS             complement(<1..347)
                     /locus_tag="VH419_17410"
                     /inference="COORDINATES: protein motif:HMM:NF023144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HEY7045452.1"
                     /translation="MSEWNAMTYEGKDTILRVVRNEAERFFALAERPDLWHAQSACDR
                     WTTADVVGHIVDTTEGYFRAFDAARGTGEVGEPHGLPAMSELANEGATSFRGASQDEM
                     MERIRTDFHKMQE"
     gene            complement(437..949)
                     /locus_tag="VH419_17415"
     CDS             complement(437..949)
                     /locus_tag="VH419_17415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY7045453.1"
                     /translation="MLDETAREDLEARREELRQKYLKPRTEQESTPGRGIHHAALICS
                     DVEQTIQFYQGLLGFPLVELVENRDYQGSSHFFFDLGNQTLLGFFDFPGLGLQPGVES
                     IGSVQHIAISCPRDRWEQLRARLDDAGVEYAGPDRGIEESMYFKDPDGIQIELLSDPL
                     MYFAGRQLDE"
     gene            1249..1995
                     /locus_tag="VH419_17420"
     CDS             1249..1995
                     /locus_tag="VH419_17420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7045454.1"
                     /translation="MPRKRPLIAILNESTLITDDEIAPIVAAAQRAVDENFEPAWGAT
                     ARLRQIPSGESPPAKAWWIGFFDDSDVANALGYHDVTDEDLPLGKAFVRTVQHYGASL
                     SVDFTHELFEMLADPYVMLDVQIDNKGTRFAHEVCDAVEADELGYDVDGVLISDFVTP
                     EWFAPHFDGPYDLKEHTSKALQLLPGGYIGVWTPEKGWSQRFGKDDGGVTDELADQPS
                     RIWPRLGSRRQRRGRRPKRSDPQNHPTTNP"
BASE COUNT          478 a          949 c          954 g          455 t
ORIGIN      
        1 atctcctgca tcttgtggaa gtcggtacgg atccgctcca tcatctcgtc ctgagaggcg
       61 ccgcggaacg acgtcgcgcc ctcgttggcc agctcgctca tcgccggcag gccgtgcggc
      121 tcaccgacct cgccggtccc gcgtgcggcg tcgaacgcgc ggaagtagcc ctcggtcgtg
      181 tcgacgatgt gaccgaccac gtcggccgtg gtccagcggt cgcacgcgga ctgcgcgtgc
      241 cacaggtcgg ggcgttcggc aagggcgaag aagcgctcgg cctcgttgcg gaccacgcgc
      301 aggatcgtgt ccttgccctc gtacgtcatg gcgttccact cactcatgtt cgacacctct
      361 ctcggtgagg acccccgtgg attcgactgt cagacaaggg cggacaagac ggcggacgag
      421 accgtacgcc ggaccgtcat tcgtcgagct gacggcccgc gaagtacatc aacgggtcgc
      481 tcagcagctc gatctggatg ccgtcggggt ccttgaagta catcgactcc tcgatcccgc
      541 ggtcggggcc ggcgtactcc acacccgcgt cgtccagccg ggcccgcagc tgctcccacc
      601 ggtctcgggg acacgagatc gcgatgtgct ggacgctgcc gatcgactcg acacccggct
      661 gcagtccgag cccggggaag tcgaagaagc ccagcagggt ctggttgccg aggtcgaaga
      721 agaagtgcga cgagccctgg tagtcgcggt tctcgaccag ctcgaccaac gggaagccca
      781 gcaggccctg gtagaactgg atcgtctgct cgacgtcgga gcagatcagt gccgcgtggt
      841 ggatcccacg tcccggcgtc gactcctgct ccgtccgcgg cttcagatac ttctggcgca
      901 gctcttcacg tcgagcctcc aggtcctccc gcgcggtttc gtccagcatg cttcacactc
      961 ctgcggctcg cgacggcatc tcactggcga ctgtagtgct cgccgtcgac cagtggaaga
     1021 tgaatgtgca accactcgcg tacgcccgac tgagcccctt gacaacgcac cgacgcgcga
     1081 gtcccctcgt cagtgagaag gccgtcggac cggcgccagg agggcgcgga ggaccggccg
     1141 gatccacccg aaacccgggc cacggagcat gcctggcgcc tcgtcgcgcc gcagccagag
     1201 gttgccgcgt gacccctgac agacgtcgtg tacgtcagag gaggggacat gccacgcaag
     1261 cgtccgctga tcgcgatcct gaacgagagc acgctgatca ccgacgacga gatcgcgcca
     1321 atcgtggcgg ctgcgcagcg ggccgtcgac gagaacttcg agcccgcctg gggcgcaacc
     1381 gcacggctcc gccagatccc ttcgggcgag tcgccaccgg cgaaggcctg gtggatcggg
     1441 ttcttcgacg actccgacgt tgcgaacgcg ctgggctacc acgacgtgac ggacgaggac
     1501 ctgccgctgg gcaaggcgtt cgtccgtacg gtgcagcact acggcgcctc gctcagcgtc
     1561 gacttcacgc acgagctctt cgagatgctc gccgatccgt acgtcatgct cgacgtgcag
     1621 atcgacaaca aggggacgcg gttcgcgcac gaggtctgcg atgcggtgga ggcggacgag
     1681 ctcgggtacg acgtcgacgg cgtcctgatc agtgacttcg tgacgccgga gtggttcgca
     1741 ccccatttcg acggtcccta cgacctcaag gagcacacga gcaaggcgct gcagctgctg
     1801 ccgggcggct acatcggtgt gtggacgccg gagaaggggt ggagccagcg gttcggcaag
     1861 gacgacggcg gggtcaccga cgagctggcg gaccagccgt cccggatctg gccgcgcctc
     1921 gggtcccgcc ggcagcgacg cgggcgccgc cccaagcgca gcgaccccca gaaccacccg
     1981 acgaccaatc cctgaccaac gaccctggtc agtggttccc cgggctcatg cgggaagagt
     2041 gggggactcc tcgggcttct gtgggcggag aggtcgcgat tggcgggcgt ggtcgcccgg
     2101 cccgccgtcg gctttcggcc gaggtggaac ggcagatgag agcgggaggt ggggctgcgc
     2161 gccgagctgg ccggccggct ttcggcccct tggtggtcgg tgtgtgggtg gcggttttcg
     2221 gacatctgcg gctgacccac ctgctggttg cgatgtccac atcccaaacc cactgtgccc
     2281 atccaaggtt tgcgatgttt acacgccttt tgggctgtaa acatcgcaaa cctcgaagca
     2341 gggatgtccg attggtgcgg tagacggcct tccgaggcgg ggggttggcc gcccaccggc
     2401 atttcggaaa ccactttcac cgccagtcgg agacgccgat ggtggtttgc ctgggtagcc
     2461 atccagccgc acgggtggcc aggcagacct gccttgggac cggtggggtt gcctgggtag
     2521 ccatgcaaac cgccgctcgg cgaccggcgg atgtccgaaa cccggcagtc agaccaggca
     2581 aagggacaga agtgctgccc accggctcag cgggcagcaa cactcccgct cttcgctgcc
     2641 atcccctcgc ccccaggcaa ggggcagccg gacgaccacg cccgccaatc gcaaccatct
     2701 ccgcaaacag aagcccagaa gtgtcgctgc gagccgtccg aaggcaggga gactcccgca
     2761 ctcgtggacc tcgtcggtgg gccgggctgt atcgcgcgtg cggagcatgc tgggctaacc
     2821 tcttctaagg taaggc
//
LOCUS       DATBFZ010000568         1542 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_6491911, whole genome shotgun sequence.
ACCESSION   DATBFZ010000568 DATBFZ010000000
VERSION     DATBFZ010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6491911"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..840
                     /locus_tag="VH419_17425"
     CDS             <1..840
                     /locus_tag="VH419_17425"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017936602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY7045455.1"
                     /translation="VVRAAGAVVLRRGHVLLVHRPRYDDWSFPKGKLDRRELTPVAAV
                     REVGEETGVRVRLGVPLGSQSYPNGRGTKIVDYWVGRVVGDADVSGYLVNAEIDEVAW
                     VPVDKAEKRLTYSRDGSTLREALATGKKTRAVVVLRHGKSRSRKAWRKDDWKRPLLVA
                     GAAQARALVPVLAAYGVTRIVTSSSARCADTVVPYAESTGWPVVRTDALSEEDATDTS
                     VLGIVDELLHSDEDAVLCTHRPVLPTVYDSIGVPAEHHATGEMVVVHHRKGHVRAVER
                     HLV"
     gene            1020..>1542
                     /locus_tag="VH419_17430"
     CDS             1020..>1542
                     /locus_tag="VH419_17430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017936601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEY7045456.1"
                     /translation="MNTSSLRRIALPAVAALTVGLTLTACGDKNSGSSSGGDTLSGGG
                     ATSQANAQQTWRSNYQKQNGGTINYEEVGSGTGVENFSSGAYSFAGTDAYLTSDQMSA
                     AKSQCGADPIEVPAYVSPIAVAFNLSGVKSLNLDAKTVADIFNGKVTKWNDPENASLN
                     SGAKLPDTAISTVH"
BASE COUNT          250 a          565 c          513 g          214 t
ORIGIN      
        1 gtggtcaggg ctgccggtgc cgtggtcctg cgccggggac atgtcctgct cgttcaccga
       61 ccgaggtacg acgactggtc gttccccaag ggcaagctcg accggcgcga gctcacgccg
      121 gtcgcggccg tccgcgaggt cggcgaggag accggcgtcc gggtgcggct gggcgtgccg
      181 ctcggcagcc agtcctaccc caacggccgt gggaccaaga tcgtcgacta ctgggtgggc
      241 cgcgtggtgg gggacgccga cgtgtccggc tacctcgtca acgccgagat cgacgaggtg
      301 gcctgggtgc cggtcgacaa ggccgagaag cggctgacct acagccgtga cggcagcacg
      361 ctccgcgagg cgctggccac cggcaagaag acgcgggcgg tcgtcgtgct ccggcacggg
      421 aagtcgcgct cccgcaaggc gtggcgcaag gacgactgga agcgtccgct tctcgtcgcc
      481 ggcgccgcac aggcgcgggc cctggtgccc gtgctcgcgg cgtacggcgt gacccggatc
      541 gtgacctcgt ccagcgcgcg ctgcgcggac accgtggtgc cgtacgccga gagcaccggc
      601 tggccggtcg tccggaccga cgcgttgtcc gaggaggacg ccaccgacac ctcggtgctg
      661 ggcatcgtgg acgagctcct ccactccgac gaggacgccg tcctctgcac gcaccggccg
      721 gtgctcccca ccgtgtacga ctccatcggc gtccccgcgg agcaccacgc gaccggcgag
      781 atggtcgtcg tccaccaccg caagggccac gtccgagccg tcgagcgcca cctcgtctga
      841 ccccgccgtc accgcgcggg cgtacccccg gcgttcacat cgctgtcgct tctcagggac
      901 ccagatgttg cgatctgggc gccctgagtt caccgctcgt tcaccgacgc cgccatgacc
      961 gtccacctcg cgtgcctacg ttccgagatg tccgagacga actgactcag gagcaccacg
     1021 tgaacacctc ttccctgcgc cggatcgccc ttccggctgt cgcggccctc accgtgggtc
     1081 tgacgctgac tgcctgtggc gacaagaaca gcgggagcag cagcggtggc gacaccctca
     1141 gcggcggcgg cgcgacctcc caggcgaacg cccagcagac ctggcgttcc aactaccaga
     1201 aacagaacgg cggcaccatc aactacgagg aggtcggctc cggcaccggt gtcgagaact
     1261 tctccagcgg cgcctactcg ttcgccggga ccgacgccta cctcaccagc gaccagatga
     1321 gcgcggccaa gagccagtgc ggcgccgacc cgatcgaggt gccggcgtac gtcagcccga
     1381 tcgccgtggc cttcaacctg tcgggcgtga agtcgctcaa cctggacgcc aagacggtcg
     1441 cggacatctt caacggcaag gtcaccaagt ggaacgaccc cgagaacgca tcgctgaact
     1501 ccggcgccaa gctgccggac accgcgatct cgaccgtcca cc
//
LOCUS       DATBFZ010000569         2056 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_6495742, whole genome shotgun sequence.
ACCESSION   DATBFZ010000569 DATBFZ010000000
VERSION     DATBFZ010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6495742"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..932
                     /locus_tag="VH419_17435"
     CDS             <1..932
                     /locus_tag="VH419_17435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020578160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070005 - cysteine-type aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HEY7045457.1"
                     /translation="WNMFVALVRKHGLVPKAAMPETHSSSKTGSLNRSIAQLMRKTAR
                     DLRAQAADGASEEQLHDSKLAALEVMHRMLSIHLGTPPTTVQWQWNDKDKTFHRDETM
                     TPQEFAEKYVALPVDDYVCIVHDPRASSERGRTYTVEYLGNVVGAPPVVYLNVDMAAI
                     KELAMAALLTGEPVWFGCDTGKMSNLERGYWDERLYDYDAVYDADLGMDKADRLVHHE
                     TLMTHAMLFTGVDVVSGADGSNVPRRWRVENSWGDEKAEKGFWTMNDNWFGEHVFEIA
                     VRRSALSPELQQALDQEPIVLPAWDPMGALALA"
     gene            complement(1013..>2056)
                     /locus_tag="VH419_17440"
     CDS             complement(1013..>2056)
                     /locus_tag="VH419_17440"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HEY7045458.1"
                     /translation="LSAVRQLRVAETTWLVVLCALALLMVLCQREFPEWWPLTGYVIP
                     LVLAMNVLSIGRLLVLDVVVAACLIVSLTQVVELTALRWAATGVIAIVMIVVLWYAHD
                     RQRLGVASARGESMLIDLRDRLASQSQLPALPRAWHAEAVMRSAGGAKFAGDFIVAAK
                     SSEGNVFEVAVVDVSGKGVNAGSRALLLSGAFGGLLGALPPEQFLPAANEYLMRQEWS
                     EGFATAVHLSVNLLTGDFETRTAGHPPALQFQAGSGRWVVHWTEGPVLGLVHRAEYTT
                     HRGRMTPGDALLLYTDGLVETPKRDITYGIDKLLGEAERLVQLGFVHGADRLVASVES
                     EGDDRALFLLHRR"
BASE COUNT          376 a          716 c          674 g          290 t
ORIGIN      
        1 agtggaacat gttcgtcgca ctcgtccgca agcatggcct ggttcccaag gcggccatgc
       61 cggagacgca ctcgtcgtcg aagaccggca gcctcaaccg ctcgatcgcc cagctgatgc
      121 gcaagaccgc acgcgacctg cgtgcgcagg cggctgacgg ggcgagcgag gagcagctgc
      181 acgacagcaa gctggctgcg ctcgaggtga tgcaccggat gctcagcatc cacctcggta
      241 cgccgccgac gaccgtgcag tggcagtgga acgacaagga caagacgttc caccgcgacg
      301 agacgatgac gccgcaggag ttcgccgaga agtacgtcgc gctgccggtc gacgactatg
      361 tctgcatcgt ccacgacccg cgggcctcga gtgaacgcgg ccgcacctac acggtcgagt
      421 acctcggcaa cgtcgtcggt gccccgccgg tcgtctacct caatgtcgac atggcggcca
      481 tcaaggagct cgcgatggcg gccctgctga ccggcgagcc ggtctggttc ggctgcgaca
      541 ccggcaagat gtccaacctc gagcgcgggt actgggacga gcgcctctac gactacgacg
      601 cggtgtacga cgccgatctg ggcatggaca aggccgaccg cctggtccac cacgagacgc
      661 tcatgaccca cgcgatgctc ttcaccgggg tcgacgtcgt gtccggtgcc gacgggtcga
      721 acgtgcctcg ccgctggcgg gtcgagaaca gctggggcga cgagaaggcc gagaagggct
      781 tctggacgat gaacgacaac tggttcggcg agcacgtgtt cgagatcgcc gtacgccggt
      841 cagcgctcag tccagagctc caacaggcgc tcgaccagga gccgatcgtc ctcccggcct
      901 gggatccgat gggcgccctc gccctcgcct aactggttgc cgcgaattgc ggcaaagttg
      961 ctgcccacac ccgcgtgtcg acctccgttt tcccgcaatt cgcgggaccc cgctaccgcc
     1021 ggtggaggag gaacaaggcg cggtcgtcgc cctcggactc gacgctggcc acgagtcggt
     1081 cggcgccatg gacgaagccg agctgcacga gccgttccgc ttcaccgagc agcttgtcga
     1141 tgccgtaggt gatgtctcgc ttcggtgtct cgacgagtcc gtcggtgtac agcagcagtg
     1201 cgtcacccgg cgtcatccgg ccgcgatggg tcgtgtactc cgcccgatgc accaggccga
     1261 ggactggccc ctcggtccag tgcaccaccc agcgtccgga gccggcctgg aactgaaggg
     1321 caggcggatg cccggcggtc cgcgtctcga agtcgccggt gaggaggttg accgacaggt
     1381 gtaccgcggt ggcgaagccc tcgctccact cctggcgcat cagatactcg ttcgccgccg
     1441 gcaggaactg ctcgggcggc agtgcgccga ggagtccgcc gaacgcgccg gagagcagca
     1501 gcgcacgcga cccggcgttg acccccttgc cggacacgtc gacgacggcg acctcgaaca
     1561 cgttcccttc gctggacttc gcagcaacga tgaagtcacc agcgaacttc gccccgccgg
     1621 ccgaccgcat cacggcctcg gcatgccacg cccggggcag cgccggcagc tggctctggc
     1681 tggccaaccg gtcccggagg tcgatcagca tggactcacc ccgggcggag gcgacgccga
     1741 ggcgctgtcg gtcgtgcgcg taccacagca ccacgatcat cacgatcgcg atcacccccg
     1801 ttgcggccca ccgcaacgcg gtcagctcga caacctgggt gagcgacacg atcaggcagg
     1861 ccgccaccac cacgtccagg accagcaacc ggccgatcga caagacgttc atggcaagca
     1921 ccagagggat gacatagccg gtgagcggcc accattccgg gaactcgcgc tggcagagca
     1981 ccatgagcag ggcaagggcg cacaacacca ccagccatgt ggtctcggca acccgcagct
     2041 ggcgaaccgc cgacag
//
LOCUS       DATBFZ010000570         1565 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_5842314, whole genome shotgun sequence.
ACCESSION   DATBFZ010000570 DATBFZ010000000
VERSION     DATBFZ010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5842314"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1565
                     /locus_tag="VH419_17445"
     CDS             <1..>1565
                     /locus_tag="VH419_17445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7045459.1"
                     /translation="RFADPPPEYRPIDGWWWEAGQLDREKLRWQLEEFRDKGIAGTWF
                     YARYLYDEPLGSQPGYMSPGWWEYTRFVAEEHKRLGMIDWFSNWTALQFEQDAIRKQR
                     DAGEPALWGRRLVLHRGSQGQDGTVRIDLPEGDEVLDAAAYRVTDRSVDHASRIDLND
                     VVEGRSISWPASGAGWEVLVVAARPWDLDYVNPEIGRRWTDVILGQYERNLDGLLGDV
                     VQAYGPDEMELLNGTTIFSPDVLERVSAELGQDARPLLVGMFLDIGPQTSRIRTNYHG
                     TVGSLLGDRFYDEPTRWLHDRGMLHVTLSQRNEDAIAHAFHHGDFFRYLRSFDAPGNE
                     DPLQSPPGGRRLFRTKISSSVAHLYERQRVVVLAHYASGWGHTPEENVGWTNEAYAKG
                     ANLYSRHLGSYSLLGGWYEYVPPHDHFFHPYWRYWKTFADYVHRLSFVMSQGVHRADV
                     ALLYPLTTIHAHWKAVEELDAVEADDVDRDPFGRQAASQVFEPFAYEVSATLEHLATA
                     IYHSGIDFDFVDD"
BASE COUNT          265 a          510 c          536 g          254 t
ORIGIN      
        1 gcggttcgcc gacccgccgc ccgagtaccg tcccatcgac ggctggtggt gggaggccgg
       61 tcaactggac cgggagaagc tccggtggca gctcgaggag ttcagggaca agggcatcgc
      121 cggaacgtgg ttctacgccc gatacctgta cgacgagccc ctcgggtccc aacccggcta
      181 catgagcccg gggtggtggg agtacacacg gttcgtcgct gaggagcaca agcgcctcgg
      241 gatgatcgac tggttctcga actggacggc gctgcagttc gagcaggacg cgatccgcaa
      301 gcagcgcgac gctggtgagc cagcgctgtg gggtcgccgg ctggtccttc acagaggaag
      361 tcagggccag gacggcacgg tccggatcga ccttcccgag ggcgatgagg tcctggacgc
      421 tgccgcctat cgcgtgaccg accggtcggt cgaccacgcc agccgcatcg acctgaacga
      481 cgtggtggag ggccgatcca tatcgtggcc ggcgagcggc gctgggtggg aggtcctggt
      541 cgtcgccgct cgcccctggg acctggacta cgtgaacccc gagatcggcc gccggtggac
      601 ggacgtgatc ctcggccagt acgagcggaa cctcgacggg ctgctcggag atgtcgttca
      661 agcgtacggg ccggacgaga tggagctcct caacggcacc acgatcttct cgcccgatgt
      721 gctcgaacgg gtgtcggcgg agctggggca ggacgccagg ccgttgctcg tcggcatgtt
      781 cctcgacatc gggccgcaga catcccggat ccgtaccaac taccacggaa ccgtgggatc
      841 gctgctcggg gatcggttct acgacgagcc caccagatgg ctgcacgaca gggggatgct
      901 ccacgtcacg ctctcgcagc ggaacgagga cgccatcgcg catgcgttcc atcacggcga
      961 cttcttccgg tacctccggt cgttcgacgc gccgggcaac gaagatcccc tgcagagccc
     1021 cccgggcggt cgccgcctgt ttcggacgaa gatctcgagc tcggtggctc acctgtacga
     1081 gcgtcagcgg gtggtcgtgc tggcgcacta cgcctcaggg tggggacaca cgccggagga
     1141 gaacgtcggc tggacgaacg aggcctacgc caagggcgcc aacctgtact cacggcatct
     1201 gggctcctac agtctgctcg gcgggtggta cgagtacgtg ccgccacacg accacttctt
     1261 ccatccctac tggcggtact ggaagacatt cgccgactac gtgcaccgcc tgtcgttcgt
     1321 gatgagccag ggggtgcacc gcgcggacgt cgcgttgctg tatccgctca ccaccatcca
     1381 cgcacactgg aaggccgtcg aggagctcga cgccgtcgag gcggatgacg tggaccgcga
     1441 tccgttcggg cggcaggcgg cgtcacaggt cttcgagccg tttgcctacg aggtgtcggc
     1501 cacgctcgag cacctggcca ccgcgatcta ccactcgggg atcgacttcg acttcgtgga
     1561 cgacc
//
LOCUS       DATBFZ010000571         4456 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_6503577, whole genome shotgun sequence.
ACCESSION   DATBFZ010000571 DATBFZ010000000
VERSION     DATBFZ010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 4456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4456
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6503577"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="VH419_17450"
     CDS             <1..258
                     /locus_tag="VH419_17450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020386145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY7045460.1"
                     /translation="LSRSLFSQFVPRGREAEYFSLYQAAERGTSWLGTLLFGLVHQWA
                     DSYRPAIVALMVFFVIGIVLLSRVDVRRGIADAGNRAPAVV"
     gene            502..906
                     /locus_tag="VH419_17455"
     CDS             502..906
                     /locus_tag="VH419_17455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007077757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein RbpA"
                     /protein_id="HEY7045461.1"
                     /translation="MSERATLRGSRLGGTSFEDESGIEFAPRQRVFYDCPNGHEFDIT
                     MAAEAEVPFTWECPRCGADSRQRDGEQPEAKAEKPARTHWDMLLERRSIPELEDILSE
                     RLELLRSGVIGPAHLHRPGRSGSARSKTKKSA"
     gene            complement(1013..1519)
                     /locus_tag="VH419_17460"
     CDS             complement(1013..1519)
                     /locus_tag="VH419_17460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012921211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HEY7045462.1"
                     /translation="MPLILFILFIGVPILEIYVIIQVGQVIGALPTLVLLIVESILGA
                     WLIKHEGRRAWDALNEAIRTGQMPGRQLADAALVLVGGTLLLTPGFVTDVFGFFFVLP
                     FTRAIARRIFGFLIARGVLRRFGPTVRVYRHFGDPGTSYRDDRDRGRPPQPAPHDGDV
                     HQIYRPEE"
     gene            complement(1529..2305)
                     /locus_tag="VH419_17465"
     CDS             complement(1529..2305)
                     /locus_tag="VH419_17465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002545252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HEY7045463.1"
                     /translation="MSAPQQPTEPARPERFLVIIPTYNEAENLELVVDRVRASAPALD
                     VLVVDDASPDGTGDIADKIARHDASVHVLHRAEKTGLGAAYLAGFTWGLDAGYDVLIE
                     MDADGSHLPEQLPRLLDALPGADLVLGARWVPGGRVVNWPRRREWLSRGGNTYARALL
                     GLGLHDATGGFRAFRRTTLEGIDLSDVSSQGYCFQVDLARRTLAAGFRVVEVPITFVE
                     RVYGESKMTGEVVREALVKVTAWGARHRLDQARRLVRGRG"
     gene            complement(2305..3873)
                     /gene="lnt"
                     /locus_tag="VH419_17470"
     CDS             complement(2305..3873)
                     /gene="lnt"
                     /locus_tag="VH419_17470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEY7045464.1"
                     /translation="MPAAPTALALGRAAASTASGLAVAFAFPPYDLIWLMPFGLAGLM
                     LTVRGDPRGATPLRGRSGLLQGFCFGMGFMLPLMRWITIIGIDAWFALAVLEALFYAL
                     MGLTWAWLRPERWWPIGFAATWVGAESLRGVIPFGGLPWGSLAFGLVDTPMVRWGRVG
                     GTALVSFVVVLVVAVLVDVVERRDWGVRGLAGVGVALALAVGSAALPVGIAGAAGTTQ
                     VAAVQGNVPGEGMNPFAERRAVLDNHATATVDFAARVRAGQAVAPDVVIWPENSTDID
                     PYSDASAYEEIDSAVRAIGVPTLVGAVVDGPGPDHVRNMGIVWSPTTGPGEQYVKRHP
                     VPFGEYIPFRGLLTKFITRLDQIPRDFARGTFDSVLDLGPVKVGDVICFEVAYDGLVR
                     DAIEGGGQMLVVQTNNATYTGTGQLEQQFAISRYRAIETGRTVVVAATDGISGIIAPD
                     GSVVAQTEEKTRAVLDENVELGEGITWGLRAGPWVEAGLCALALVAVGLVLLRRPNRT
                     TRLGRRSAPAKIDA"
     gene            complement(4201..>4456)
                     /locus_tag="VH419_17475"
     CDS             complement(4201..>4456)
                     /locus_tag="VH419_17475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7045465.1"
                     /translation="AAVELVETDRVLDLDRLDQRVAAVDQRVTAVGPVAAVELVETKR
                     VGAINELATRRGYGNSRRILVDLRVALTCPFEETRRSRNG"
BASE COUNT          750 a         1583 c         1456 g          667 t
ORIGIN      
        1 ctgtcccgct ccttgttcag ccagttcgtc ccgcgtggtc gtgaggcgga gtacttctcg
       61 ctctaccagg ccgccgaacg cggcaccagc tggctgggca cgctgctgtt cggtctcgtg
      121 caccagtggg cggactccta ccgacccgcg atcgtcgcgc tgatggtgtt cttcgtgatc
      181 gggatcgtgc tgctctcccg ggtggacgtg cggcgcggca tcgccgacgc cggcaaccgg
      241 gcccccgctg tggtgtgagt cccgccgttc tccgccttac cgggcgaaat ggggcatcat
      301 ggactatggt atcggtcacg aaacgagctg ctcgggcctg gttttcagcc tggtctcagc
      361 cgggtaggga atgatctgca cggccggttc gttgggaccg gtgtcaccct gaggcgatct
      421 tcgtggcctg catccaatcc ggtggacctc gggtgacgaa ccacacgggg acccacacac
      481 cgaacgggga gtttcagcac catgtcagaa cgagcaacgc ttcgcggatc ccgtctgggc
      541 ggaacgtcgt tcgaagacga gagcggcatc gagttcgccc cgcgccagcg tgtcttctac
      601 gactgcccga acggtcacga gttcgacatc acgatggccg ccgaggccga ggtcccgttc
      661 acgtgggagt gcccacgatg cggcgcggac tcacgccagc gcgatggcga gcagccggag
      721 gccaaggcgg agaagcccgc acgcacgcac tgggacatgc tgctcgagcg gcgttcgatc
      781 cccgagctcg aagacatcct gtccgagcgg ctcgaactgc tgcgatccgg cgtcatcggt
      841 ccggcgcacc tgcaccgccc gggccggtcc ggctccgcac gcagcaagac gaagaagtcg
      901 gcctgaccca cagcatctgc acaagaggcg ccgtccgtaa cggatggcgc ctctcccgtc
      961 tgtgtcgctt ctctgggtca ctcctccttg gggtcactcc ttcggtctgg cgtcactcct
     1021 ccgggcggta gatctggtgg acgtcgccgt cgtgtggcgc cggctgtggg ggacgtccgc
     1081 gatcacgatc atcccggtac gacgtccccg ggtcgccgaa gtggcggtag acacgcacag
     1141 tcggaccgaa gcgtcgcagt accccgcgcg cgatgaggaa accgaagatc cggcgtgcga
     1201 tcgcccgggt gaacggcagc acgaagaaga agccgaacac gtcggtgacg aacccgggcg
     1261 tcagcagcag tgtccccccg acgaggacga gcgcggcgtc ggcgagctgc cggccgggca
     1321 tctgaccggt gcggatcgcc tcgttgagcg catcccaggc gcgacgacct tcgtgcttga
     1381 tgagccaggc accgaggatc gactcgacga tgagcagcac caacgtcggc agcgccccga
     1441 tcacctggcc gacctggatg atcacgtaga tctccaggat cggcaccccg atgaacaaga
     1501 tgaacaagat caacggcatc gcgcgaacct agcccctccc ccggacgaga cgccgggcct
     1561 ggtcgagtcg gtgtcgcgcg ccccacgcgg tgaccttcac cagcgcctcc cggacgactt
     1621 caccggtcat cttggactcg ccgtagaccc gttcgacgaa cgtgatcggc acctccacga
     1681 cccgaaaccc ggccgcgagc gtacgccggg cgaggtcgac ctggaagcag tatccctgcg
     1741 acgacacgtc cgacaggtcg atgccctcca acgtcgtacg ccggaacgcc cggaagccgc
     1801 ccgtggcgtc gtgcaggccg agtccgagca gtgccctggc gtatgtgttg ccgccacgtg
     1861 acagccactc gcgccgccgt ggccagttca ccacccgtcc gccaggcacc cagcgcgccc
     1921 cgagcacgag gtcggcaccc ggcaacgcgt ccaacaggcg tggcagctgc tcggggagat
     1981 gggatccatc cgcgtccatc tcgatgagca cgtcgtagcc cgcgtccagc ccccacgtga
     2041 aacccgccag gtacgccgca ccgagcccgg tcttctcggc ccggtgcagc acgtgcacgg
     2101 atgcatcgtg gcgggcgatc ttgtcggcga tgtcgcccgt accgtccggg gaagcgtcgt
     2161 cgacgaccag gacgtccagc gcaggggccg acgcccggac ccggtcgacc acgagctcga
     2221 ggttctccgc ctcgttgtag gtggggatga tcaccaggaa gcgttcgggc cgggccggtt
     2281 cggtgggctg ctggggcgct gacatcacgc gtcaatcttg gcaggggcgc tacgacgacc
     2341 caaacgagtc gtgcggttcg gccgcctgag cagcaccagg cccacggcga cgagagcgag
     2401 cgcgcacagt cctgcctcca cccacggtcc ggcgcggagt ccccaagtga tcccttcccc
     2461 gagctcgacg ttctcgtcca gcaccgcgcg ggtcttctcc tcggtttgcg ccaccacgct
     2521 gccgtccggc gcgatgatgc cgctgatccc atcggtcgct gccacgacga cggtccgccc
     2581 ggtctcgatg gcgcggtagc gagagatcgc gaactgctgc tcgagctgcc cggtgccggt
     2641 gtaggtcgcg ttgttcgtct gcacgacgag catctgcccg ccgccctcga tcgcgtcgcg
     2701 gaccaagccg tcgtacgcca cttcgaaaca gatcacgtcg ccgactttga ccgggccgag
     2761 gtccaggacg ctgtcgaagg tgccgcgggc gaagtcgcgc gggatctggt ccaggcgggt
     2821 gatgaacttg gtgagcaacc cgcggaacgg gatgtactcg ccgaacggca cgggatgccg
     2881 tttgacgtac tgctctccgg gaccggttgt cgggctccag acgatgccca tgttgcgcac
     2941 atggtcagga cccggcccgt ccacgaccgc gccgaccagc gtgggtacgc cgatcgcccg
     3001 gaccgcgctg tcgatctcct cgtacgccga ggcgtcactg tacggatcga tgtcggtcga
     3061 gttctccggc cagatcacga cgtcgggtgc cacggcctgt ccggcgcgca ccctggccgc
     3121 gaagtcgacc gtcgccgtgg catggttgtc gagcaccgct cgccgctcgg cgaacgggtt
     3181 cattccctcc ccaggcacgt tgccctggac cgcagcgacc tgcgtggtgc cggctgcgcc
     3241 ggcgatcccg accggcaggg ccgcgctgcc gaccgcgagc gcgagcgcca cgccgacacc
     3301 agcaagacca cggacgcccc agtcgcgacg ttcgaccacg tcgaccagga ccgccaccac
     3361 gagcaccacg acgaaggaga ccagcgcggt tccaccgact cggccccagc ggaccatcgg
     3421 tgtgtcgacg aggccgaatg ccaggctccc ccagggcagc ccgccgaagg ggatcacccc
     3481 gcgcaacgac tcggctccca cccacgtcgc cgcaaaaccg atcggccacc accgctccgg
     3541 gcgcagccac gcccaggtca gtcccatgag cgcgtagaac agcgcttcga gcaccgccag
     3601 cgcgaaccac gcgtcgatgc cgatgatcgt gatccaccgc atcaacggca gcatgaagcc
     3661 cataccgaag cagaacccct ggaggaggcc cgaccggcca cggagcgggg tcgctcccct
     3721 cgggtcgccg cggacggtca gcatcagccc ggccaggccg aacggcatca gccagatcag
     3781 gtcgtacggc gggaaggcga acgcgacggc cagaccggag gcagtggagg cggccgcccg
     3841 gcccagggcc aacgcagtgg gggcggcggg cacgcgacga gcctaccttt gccgctcaag
     3901 gaactcgccg gctgatcgag cctgtcgaga tcgtggaccg gatcgccgtc ggccccagaa
     3961 acggtacgag cccgaccccc ttcggaccga acgtggcctc actggcggtg aagccggacg
     4021 cccgttgtct actggcggtc gagctcattc cgggtccact ctcccgaggc gggccgcttc
     4081 ccggacgtcg cgctgctgct gagtttggat cgaaaaccag gttgcacgac gacccgggtg
     4141 ctgcgctctc gacagcctgc tcagtggacg gcgcgactct gccaaccaat ggcctgcctg
     4201 tcaaccgttt cgtgacctgc gcgtttcctc aaaggggcag gtcagcgcaa cccggaggtc
     4261 gacaagaatt cgccgggagt ttccgtagcc gcggcgtgtc gccagttcgt tgatcgcacc
     4321 aacgcgtttg gtctcgacaa gctcgaccgc cgcgacaggc ccgaccgccg taacccgctg
     4381 atcgaccgcc gcaacccgct gatcgagcct gtcgagatca aggacccgat cggtctcgac
     4441 aagctcgacc gccgcg
//
LOCUS       DATBFZ010000572        10539 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_5851348, whole genome shotgun sequence.
ACCESSION   DATBFZ010000572 DATBFZ010000000
VERSION     DATBFZ010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 10539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10539
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5851348"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1480
                     /locus_tag="VH419_17480"
     CDS             <1..1480
                     /locus_tag="VH419_17480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017934844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HEY7045466.1"
                     /translation="NAKYHEREATIVAQAGRKGAVTVATNMAGRGTDIMLGGSVEFLA
                     DEELRRQGLDPLEHSDDYEAAWPATLEKIEAQVGAEHDDVNDAGGLYVVGTERHESRR
                     IDNQLRGRSGRQGDPGESRFYLSLEDDLMRLFKSDWVDWVLTTMKIPDDVPIENKRVT
                     GAIASAQAQVESQNFETRKNILKYDDVMSRQREVIYGERRQVLEGADLHEQIRAMLDD
                     TVEGYVNGATEGFPEDWDLDALWTALKTLYPVSLSQQQLVDDAGGVEGLDRDYLAEEL
                     KADAHAAYDAREESLGSEVMRELERRVLLSVLDRKWREHLYEMDYLREGIGLRAYSQR
                     DPLVEYQREGFELFNAMMDSIKEESVGFLFNVEVSVDTEETPSDEAAVQNAAAALVAG
                     EVPAQPTIPEGRPDAEPAHDDHRPHITARGLAAPQAPRKLTYSAPSVDGDAGTTIQAE
                     QGAPGSSAADDEFAGVSRNELCPCGSGRKFKRCHGDPARRNA"
     gene            complement(1535..2059)
                     /locus_tag="VH419_17485"
     CDS             complement(1535..2059)
                     /locus_tag="VH419_17485"
                     /inference="COORDINATES: protein motif:HMM:NF039460.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rv3235 family protein"
                     /protein_id="HEY7045467.1"
                     /translation="MTEQPLRTLTAPASRRWQLIDDSATSTYTQGALALSYPVPGGDE
                     SGSAAAGLSLVRPDRDIDTEIPDPEPWAARFIQAVLEVVSSDRPLTQLARWTAARVYA
                     DLSRRRDRVARHRRPGQLRATRQHVATVHVWQPSRDSAEVAARVTMGGRSRAIAARLD
                     FRRGRWLCTALTFG"
     gene            complement(2056..2589)
                     /locus_tag="VH419_17490"
     CDS             complement(2056..2589)
                     /locus_tag="VH419_17490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HEY7045468.1"
                     /translation="MKANTGIDALVVLLIAPIVTVVAAITLLCLAAAIAEAVLTRIAP
                     LRPWRWSPAPHVLRRLAYAACGATLALPAATAAAVPAARSTTIDRTCPPVCVGRLEGL
                     PLPDLPTSVPAADPIRVEPGDSLWLIATGLLDARADDSQVARAVARLYRTNRSVIGAD
                     PDLIFPGTSLTIPEVLR"
     gene            2681..3253
                     /locus_tag="VH419_17495"
     CDS             2681..3253
                     /locus_tag="VH419_17495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7045469.1"
                     /translation="MRWRDLFSDLEGQAAAWEQRERDVEVADRSRAEQGRVALVERVS
                     AATGRDLLVRLHGGAQVHGTIVTVGADWMLVERTDPVEESLVLLGAVLGIADLDRRAH
                     SEAARGVVMSRLRVASPLRAMARDRSAVRVVLTDGSLLAGTLQLVGKDHLDLLLHDVE
                     EAPRPEHARGRLTVPLQALAVLHRGSSRWT"
     gene            complement(3286..3549)
                     /locus_tag="VH419_17500"
     CDS             complement(3286..3549)
                     /locus_tag="VH419_17500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY7045470.1"
                     /translation="MADAPKPRFLALADVAEILQISVAQAYALVRRGELPAIKVGGRG
                     QWRVEIDALESYIADAYSATKTYIEQHPYTSDDTSADELADRE"
     gene            3650..4342
                     /locus_tag="VH419_17505"
     CDS             3650..4342
                     /locus_tag="VH419_17505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7045471.1"
                     /translation="MSLSHRDVTPRADVDRAPGQGTSPPAQRLASRRWRDPRLWAGVL
                     LVLVSVLAGAALLASSDDTVGVWAADADLHAGMSITPGDVHVVRVHFTDAASSDRYLD
                     ASHTIPDGAVLTRDVLAGELLAVAALDDGATEQDQLPLAVTPAGLPAGLAVGDKVDVW
                     AMPAPETAADGAQASRQVLDGVVVTALGSPGPGGLESARNVLVGIPDRTDVGEVLDGL
                     RGADVVLVRVGG"
     gene            4365..5591
                     /locus_tag="VH419_17510"
     CDS             4365..5591
                     /locus_tag="VH419_17510"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7045472.1"
                     /translation="MGVLVAVGSAPFEADLLTALDAPGLHVVRRCVDVADLLATATAR
                     QAEVALVSAQLRGLDATVVARLRHEAVSVLGVTVEAASADEARLRELGIVDVVPADRL
                     DELESTVAGVTSDVATHRLAEEPDGHVRVPRAATTVAVWGPTGAPGRSTLAVGMAAAA
                     ADLGRRTLLVDADVYGGAVAPMLGLLDEASGLLAAARAANHGELGPDILDRTARTVNP
                     TLRVLTGLPRADRWVEVGSTLIRRVIDTAADVSDLVVVDCGFSLEADEDLSYDTAAPR
                     RNGATLEVLSGADVVIALGGADPVGLSRLIRAVHDLRTTFPGVQPRIVVNRVRPSLGW
                     SDEEVSAMIMRATGLTVTATLPDDPASCDRALVQGKTLTECASDGRLTRRIHAVTADL
                     LGLRQPVTRRRAGTVR"
     gene            complement(5560..6918)
                     /locus_tag="VH419_17515"
     CDS             complement(5560..6918)
                     /locus_tag="VH419_17515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008359773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7045473.1"
                     /translation="MSDSVQAPHTSVLAHGVGGPQDLPIPAHYAFVGAVVALVVSFAV
                     LGFAWRTSRFRGDESGRPLPGWLARFVDSPVTRTTLVALSLTFTAWVVLAALFGSDTL
                     VNPTFGVVFVLLWVGLVPAALLFGFIYRLCNPLRWVHRGICAAAAIDHREGLRTYPER
                     LGYWPGAFFLFAFVWLELVDPQISTSLTAIRLWFGVVGAVLLAGAAVFGDTTFARAGP
                     FEVYSGMVARLSPFGRRTDGVLVVRNPLQNLDGLTPRAGLVGVAAVLFGSTAFDSFKE
                     SSRWLRFSQDYTGHMVLLNTLALLTFCLIVLVTFSLAAVMTGGLGHAVGIRRRDLPNL
                     MAHSVVPIVVGYIVAHYLSFFASTGVATLQQLGDPLSRGWTLTSFLDDVNKYAIYEHP
                     TALAVTKVVAVVTGHVLGVVAAHDRAVRILPKRHALVGQLPMLVLMVAYTLTGLFLLF
                     SS"
     gene            complement(6937..7314)
                     /locus_tag="VH419_17520"
     CDS             complement(6937..7314)
                     /locus_tag="VH419_17520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7045474.1"
                     /translation="MRAISRALGVVLAITALTLGLGGCSDDSSDGGSDSSKPVTVDIT
                     EQNGKVQPVGKVVNAAKGQDITLVVTSDADDEIHVHSEPEHEFQVEAGDTDKRFTFSV
                     DIPGTIEVESHGLDVTILKLQIS"
     gene            7481..8941
                     /locus_tag="VH419_17525"
     CDS             7481..8941
                     /locus_tag="VH419_17525"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012919308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HEY7045475.1"
                     /translation="MPERLRPADMTFLAMESARTPMHVATLDIFEPADDGFDYERLVA
                     LINDRIAFVPRYRQRVRQVPGRLANPVWVDDEDFDLAYHVRRSALPRPGSMHQLRELV
                     ARIMSRRLDRDRPLWEVYLVEGLEAGRYAVLSKSHQVLVDGATVDLGQLIVDDSPQAP
                     VTPDVGWRPERQRRGVELLGDAFAESVRAPAVAADNVRGQLVGFRFAGERLRRFAWGR
                     PTTSPGPLGAEPSEQRRFVTVETRLSAYRKVRQQHGGTVNDVVLATVAGALRGWLLTR
                     AESVTTSTRIKAMVPMSVVDDEFAEPTSLGSQVTPHLLTLPAGEPNPVMRLHQVSYAF
                     KAHKETGRAVAADKLAELSGFAPSTFHAIGARLAASEASRSFDLVVTNVPGPQFPLYA
                     AGARLQASYPVPPLLAGQALAIGVTSYNGKVFYGLNADRDAMPDIDVLAACVSDALDE
                     LVDASSSTRARAPRGKVKRAATKKAPRPKASAGEGS"
     gene            8941..9381
                     /locus_tag="VH419_17530"
     CDS             8941..9381
                     /locus_tag="VH419_17530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7045476.1"
                     /translation="MRVYVAATIERLQSWHDDQVLQAPVAAFAVTDDLRTELPDLGDE
                     EAEYAVATAAAEASGQMLTDGMHKRGRRVVVVVEVSYSVVEPDDETPGAVNVVAPIEV
                     QHIAAVLADPVDIPLTGGGEMDDLAWFAVQEIPELLVSKHDGDQ"
     gene            9398..10405
                     /locus_tag="VH419_17535"
     CDS             9398..10405
                     /locus_tag="VH419_17535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020577276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEY7045477.1"
                     /translation="MAQAIWKGSISFGLVTIPVKLYSATEEKDISFRQVHEEDGGRIR
                     YRRVCEIDGEEVPYSDIGKGYEMPDGRMVILTNDDFADLPLPTAKSIEVLEFVPIEQV
                     DPLFFAKAYYLGADGGPGGKPYVLLRDALTKTKQCALVKVAIRTKEVLALLREKEGAL
                     VLQTMLWPDEVRDAGFAAPDDSIEIRKQEVEMAESYIATLRADFEPEKFHSDYREALE
                     RLVEAKAEGVPVPEVEEDESEPAEVVDLVAALRASVDAAKRRRTAPASGHAPAERGSP
                     TARQAGAEASAKKSRTKKSDKKAAGKKTPAKKSAAKKAAAKKSTAKKSTSKKSPVKKS
                     A"
BASE COUNT         1759 a         3465 c         3747 g         1568 t
ORIGIN      
        1 gaacgccaag taccacgagc gggaggccac gatcgtcgcc caggccgggc gcaagggcgc
       61 ggtcaccgtg gccaccaaca tggcgggacg cggcaccgac atcatgctcg gcggctccgt
      121 cgagttcctc gccgacgagg agctccgccg ccaagggctc gacccgctgg agcactccga
      181 cgactacgaa gcggcctggc cggcgaccct ggagaagatc gaggcgcagg tcggggcaga
      241 gcacgacgac gtcaatgacg caggcggcct ctacgtggtc ggcactgagc ggcacgagtc
      301 acgtcgcatc gacaaccagc tgcgcggacg ttcgggccgc cagggtgacc cgggcgagtc
      361 ccgcttctac ctgtcgctcg aggacgacct gatgcggctg ttcaagtccg actgggtcga
      421 ctgggtcctc accaccatga agattcccga tgacgtgccg atcgagaaca agcgcgtgac
      481 cggagccatc gcgtcggcgc aggcacaggt cgagtcgcag aacttcgaga cgcgcaagaa
      541 catcctcaag tacgacgacg tgatgagccg gcagcgggag gtcatctatg gtgagcgccg
      601 tcaggtgctc gaaggcgctg acctgcacga gcagatccgg gccatgctcg acgacaccgt
      661 cgagggatac gtcaacggcg cgaccgaggg cttccccgag gactgggacc tcgatgcgct
      721 gtggacggcg ctcaagacgc tctaccccgt ttcgctctcc cagcagcaac tggtcgatga
      781 cgccggtggg gtcgaggggc tcgaccgcga ctacctcgcc gaggagctga aggcggatgc
      841 ccatgcggcg tacgacgccc gcgaggagtc gctcggctct gaggtgatgc gcgagctcga
      901 gcgccgggtg ctgctgtcgg tcctggaccg caagtggcgt gagcacctct acgagatgga
      961 ctatctgcgt gagggcatcg ggcttcgtgc gtactcgcag cgagacccgc tggtcgagta
     1021 ccaacgtgag ggcttcgagc tgttcaacgc gatgatggac tcgatcaagg aggagtcggt
     1081 cggcttcctc ttcaacgtcg aggtcagcgt cgacacggag gagacgccgt ccgacgaagc
     1141 agcggtgcag aacgccgcgg cagcgctcgt cgccggcgag gtgccggcgc agccgacgat
     1201 tccggagggc cgaccagatg cggaacccgc ccacgacgac caccggccgc acatcaccgc
     1261 cagagggctg gccgcaccgc aggcgccgcg gaaactgacc tactcggcac cctcggtcga
     1321 cggtgacgcc ggtacgacga tccaggcgga gcaaggtgcg cccggaagca gcgccgccga
     1381 cgacgagttc gccggtgttt cgcgcaacga gctgtgccct tgtgggtcag gtcgcaagtt
     1441 caagcgctgt cacggagacc cagcccgccg caacgcataa ccgagttgtc agaaccccat
     1501 ggacctatga gtcgatggag ttctgacaag tcggtcagcc gaaggtgagg gcggtgcaga
     1561 gccagcggcc gcggcggaag tccagtcggg cagcgatggc tcgagagcgg ccgcccatgg
     1621 tgacgcgagc cgccacctcc gcgctgtcgc gcgacggttg ccagacgtgc acggttgcca
     1681 cgtgctgcct ggtcgcgcgc aactggccag gcctacggtg ccgggcgacg cggtcgcgac
     1741 gacggctgag gtccgcatag acccgagcgg ccgtccaccg tgccagctgg gtgaggggcc
     1801 ggtcactgct gaccacttcc aacaccgcct ggatgaagcg ggccgcccac ggttccggat
     1861 ccgggatctc ggtgtcgatg tcgcgatcgg gcctcaccag actgagcccg gccgcggcgg
     1921 agccggactc gtcgccaccg gggacgggat aggagagggc gagcgcacct tgtgtgtagg
     1981 tgctcgtcgc cgagtcgtcg atgagctgcc agcgccgaga cgccggtgct gtcagggtgc
     2041 gaaggggttg ctcggtcatc ggagtacctc cgggatcgtc agggacgtgc cggggaagat
     2101 caggtcaggg tcggctccga tcacggagcg attggtgcgg tagagcctgg cgaccgcgcg
     2161 tgcgacctgg ctgtcgtcgg ctcgtgcgtc gaggagcccg gtggcgatca gccacaggct
     2221 gtcgcccggc tcgacccgga tcgggtccgc agcggggact gacgtcggca ggtcagggag
     2281 gggaagtccc tccagcctgc ccacacagac cggcggacac gttcgatcga tcgttgtgct
     2341 ccgcgcggcc ggtacggcgg cggcagtcgc agcaggcagg gccagcgtgg cgccacatgc
     2401 ggcgtacgcg agtcgtcgca gcacgtgcgg cgccggcgac cagcgccatg gccgcagtgg
     2461 tgcgatccgg gtcagcactg cctccgcgat cgcagccgcc aggcacaaca gcgtgatcgc
     2521 ggccacgacc gtcacgatgg gtgcgatgag cagcaccacg agtgcgtcga tccctgtgtt
     2581 tgctttcatt tgctttattt agcctccgtt tggtggcgga cgtcaagttc gtgtccacag
     2641 cccgtggcgg aagcgtgcga cggcgtcgta gcgtgcggcc atgcgctggc gtgacctgtt
     2701 ctcggacctg gagggtcagg cagcggcgtg ggagcagcgt gagcgcgacg tcgaggtcgc
     2761 tgaccgaagc cgagccgagc agggacgagt ggcgctcgtc gaacgggtga gcgccgcgac
     2821 cgggcgggat ctgctcgttc ggttgcacgg cggggcgcag gtgcacggga cgatcgtcac
     2881 ggtcggcgcc gactggatgc tggtcgagcg caccgacccc gtcgaggaga gcctggtcct
     2941 gctcggggcc gtcctgggca tcgccgacct cgaccggcgg gctcactccg aagcggcgcg
     3001 tggtgtcgtg atgagccggc ttcgggtggc gtcgccgttg cgcgcgatgg ctcgtgaccg
     3061 gtcagcggtg cgcgtggttc tgacggacgg gtcgctgctg gcgggcacgt tgcagctggt
     3121 ggggaaggac cacctcgacc tgctcctcca cgatgtcgag gaggcgccgc gaccggagca
     3181 cgcgcgagga cggctgaccg taccgctgca ggccctggcc gtcctgcacc gcggctcgag
     3241 ccgctggacc tgagtacgtc gacccgcggt ctggacgcca accggtcact cgcgatccgc
     3301 gagctcgtcc gcggaggtgt cgtcgctggt gtaggggtgc tgctcgatgt aggtcttggt
     3361 ggcggagtac gcgtcggcga tgtagctctc gagcgcgtcg atctcgaccc gccactgacc
     3421 gcgcccgccg accttgatgg ccggcagctc gccacggcgt accagtgcgt aggcctgagc
     3481 aaccgagatc tgcaggatct cggccacgtc ggccagcgcc aggaaacgcg gcttcggggc
     3541 gtcggccatg cgctgcagtc tgtcacgggg ttcgacccac tcagccgccg cgactccgcg
     3601 tgtggacaac tcttcccaac cgggcgagtc tgtggcaaga atggccgacg tgtcgttgtc
     3661 gcaccgcgac gtcacgccaa gagccgacgt cgatcgagcc cccggacagg ggacatctcc
     3721 accggcgcag cgtctcgcga gtcgccgctg gcgcgatccg cgactgtggg cgggcgtgtt
     3781 gctcgtcctg gtctcggtgc ttgcgggagc tgccctgctg gcgtcgtccg acgacaccgt
     3841 tggagtctgg gcagcggacg ccgatctgca tgcggggatg tccatcactc caggagatgt
     3901 ccacgtggtg cgcgtccact tcaccgacgc agcgagctcc gaccgctacc tcgacgcctc
     3961 gcacacgatc cccgacggtg cggtgctgac tcgtgacgtg ttggcgggcg aactcctcgc
     4021 ggtcgccgcc ctcgacgacg gcgcgaccga gcaggaccag cttccgctcg ccgtcacgcc
     4081 cgccgggctg cctgctgggc tcgcggtcgg cgacaaggtg gacgtgtggg ccatgcccgc
     4141 gccggagacg gctgcggacg gggcgcaggc atcgcggcag gtcctggacg gcgtcgtcgt
     4201 caccgcactg ggctcgcccg gaccgggcgg tctcgagtca gcccgcaacg tgctggtcgg
     4261 cataccggac cgcaccgacg tgggcgaggt tctcgacgga ctccgcggtg ccgacgtcgt
     4321 cctcgtccgg gtcggtggct gacatggctc gcgcaggccg atcggtgggc gttctggtcg
     4381 cggtggggtc ggcgcccttc gaggctgatc tgctgaccgc actggacgcg ccgggcttac
     4441 atgttgtacg ccggtgcgtc gacgtcgccg acctgctcgc cacggcaacg gctcgacagg
     4501 ccgaggtggc tctcgtctcg gcccagctgc gaggactgga cgcgaccgtc gtcgcgcggt
     4561 tgcgacacga agccgtgtcg gtcctggggg taacggtcga ggccgccagt gctgacgagg
     4621 cccggttgcg tgagctcggc atcgtcgacg tcgtccctgc cgaccggttg gacgagctgg
     4681 agtccacggt cgcaggtgtc accagcgacg tcgcgacgca ccgccttgcc gaggaaccag
     4741 acggtcacgt gcgggttccg cgcgcggcca cgacggtcgc cgtgtgggga ccgaccggag
     4801 cgccgggccg cagcacgctc gcggtgggca tggcggccgc cgcggccgac ctgggtcgcc
     4861 gaacactcct cgtcgacgcc gacgtgtacg gcggcgctgt cgccccgatg ctcggactgc
     4921 tcgacgaagc gtccgggctg ctcgcagcag cccgcgccgc gaaccacggt gagctcggtc
     4981 cggacatcct cgaccgcacc gctcgtaccg tgaaccccac gttgcgcgtg ctgacgggcc
     5041 tgccccgggc cgaccggtgg gtggaggtcg ggagcacgct catccggcgg gtcatcgaca
     5101 ctgcggccga cgtgagcgat ctcgtcgtgg tcgactgcgg tttcagcctc gaagccgacg
     5161 aagacctctc gtacgacacc gccgcgccac ggcgcaatgg ggcaaccctg gaagtgctct
     5221 cgggtgccga cgtcgtcatc gccctcggtg gcgcggaccc ggtcggtctc agtcggctga
     5281 tccgggcggt gcacgacctg cgaacgacct tccccggcgt acagccccgc atcgtcgtca
     5341 accgggttcg gccgagtctc gggtggtcgg acgaagaggt gtccgcgatg atcatgcgtg
     5401 ccactggact gacggtgacg gcgacgctgc ctgacgaccc cgcgtcctgt gaccgggcct
     5461 tggtgcaggg gaagaccttg accgagtgcg ccagtgacgg gcggctcact cgtcggatcc
     5521 acgccgtgac cgcggacctg ctcggcctcc gtcagccggt cacgaggaga agagcaggaa
     5581 cagtccggtg agcgtgtacg ccaccatcag gaccagcatg ggcagctgac cgacgagggc
     5641 gtgtcgcttc ggcaggatcc gtacggcgcg gtcgtgcgcg gcgaccacgc cgaggacgtg
     5701 accggtcacg acggcgacga ctttggtcac ggcgagagca gtcgggtgct cgtagatcgc
     5761 gtacttgttg acgtcgtcga ggaacgacgt gagcgtccaa ccacgtgaca gcgggtcacc
     5821 gagctgctgc aacgtcgcga caccggtcga ggcgaagaac gacaggtagt gcgccacgat
     5881 gtagccgacc acgatcggta cgacggagtg ggccatcagg ttgggcaggt ctcgtcgccg
     5941 gatacctacg gcatgaccga gaccgcccgt catcaccgcc gccaggctga acgtcacgag
     6001 tacgatcagg cagaacgtca acagcgccag cgtgttcagc agcaccatgt ggccggtgta
     6061 gtcctgcgag aaacgcagcc agcgtgacga ctccttgaag ctgtcgaagg cggtggagcc
     6121 gaacagcact gccgccaccc ctaccagccc ggcccgtggg gtcagcccgt ccaagttctg
     6181 gagcgggttg cgtacgacga gcacaccgtc ggtacgccgt ccgaacggcg acaggcgggc
     6241 caccatgccg gagtacacct cgaacgggcc cgcgcgcgcg aacgtcgtgt caccgaacac
     6301 tgccgcccca gccagcaaca ccgcaccgac cacaccgaac cacagcctga tcgccgtgag
     6361 gctggtcgag atctgtggat cgacgagctc gagccagacg aaggcgaaca ggaagaacgc
     6421 gccgggccag tagccgagcc gttcggggta ggtgcggagt ccttcgcgat ggtcgatggc
     6481 ggcggccgca cagatgccgc gatgcaccca ccgcagcggg ttgcacagcc ggtagatgaa
     6541 gccgaacagc agcgccgccg gaaccaggcc tacccagagc agcacgaaca cgaccccgaa
     6601 cgtcgggttg acgagggtgt ccgagccgaa caatgccgcc agcacgaccc aggcggtgaa
     6661 cgtcagcgag agcgcgacca gcgtcgtacg cgtgacgggc gagtccacga accgcgccag
     6721 ccaaccgggc agtggccgac cggactcgtc accgcggaac cgcgacgtcc gccaggcgaa
     6781 cccgagcacc gcgaaggaga cgacgagcgc gacgactgcg ccaacgaagg cgtagtgcgc
     6841 ggggatcggg aggtcctgcg ggccgccgac gccatgtgcg agcaccgacg tgtgcggcgc
     6901 ctggaccgag tcactcacga tgcacgtcgg ggccgctcag ctgatctgga gcttgaggat
     6961 ggtcacgtcg agtccgtgcg actcgacctc gatcgttccc gggatgtcga ccgagaacgt
     7021 gaacctcttg tcggtgtcgc cggcttcgac ctggaactcg tgctccggct cggagtggac
     7081 gtggatctcg tcgtccgcgt ccgaggtgac cacgagcgtg atgtcctggc ccttcgcggc
     7141 attgaccacc ttgcccaccg gctggacctt tccgttctgc tccgtgatgt cgaccgtgac
     7201 cggcttggac gagtcggagc caccgtccga cgagtcgtcc gaacaaccgc ccaggccgag
     7261 ggtcagcgcg gtgatggcga ggacgactcc gagtgcacgg ctgatggcac gcatgcgagg
     7321 gcggctcctt gacgtcggtg cagcctcacg agtcgaggcc cggtcagaat cggtggtcgc
     7381 gcagtccgac ccactagacg atacgaccgt gcaaggatga aggttcccgg cggtgccgaa
     7441 tcggaccgat ccacccgtca gcccaacgga cggagacgcg atgccagagc ggttgcgacc
     7501 ggcggacatg accttcctgg cgatggagtc ggcacgtacg ccgatgcacg tcgcgaccct
     7561 cgacatcttc gaaccggccg acgacggctt cgactacgag cgcctcgtcg cgctgatcaa
     7621 cgatcggatc gcgttcgtac cgcggtaccg gcagcgggtc cggcaggtcc ccggccggct
     7681 cgccaacccg gtgtgggtcg acgacgagga cttcgacctc gcctaccacg tccgacggtc
     7741 ggcgttgccg agaccgggat cgatgcacca gctgcgtgag ctggtcgcca ggatcatgag
     7801 ccgccgtctc gaccgggacc gcccgctctg ggaggtctac ctcgtcgagg ggttggaggc
     7861 cggccggtat gccgtgctgt ccaagtcaca tcaggtgctc gtcgacggcg ccacggtcga
     7921 cctcggccag ttgatcgtcg acgacagtcc gcaggcaccg gtgacgccgg acgtcggatg
     7981 gcgtccggag cgtcagcgcc gtggtgtcga actgctcggc gacgcattcg ccgagtcggt
     8041 gcgcgcccct gccgtcgccg ccgacaacgt tcggggccag ctggtggggt tccggttcgc
     8101 cggcgagcgg ctgcgccgtt tcgcctgggg ccgaccgacg acgtcgccgg gcccgttggg
     8161 tgccgagccg tccgaacaac gacggttcgt gaccgtcgag acgcggttgt cggcgtaccg
     8221 gaaggtgcgt caacagcacg gcgggaccgt caacgacgtc gtcctcgcaa cagtggccgg
     8281 tgccctacgc ggctggctgc tcacgcgcgc ggagtccgtg acgacgtcga cccggatcaa
     8341 ggcgatggtg ccgatgagtg tcgtggacga cgagttcgcc gagccgacgt cacttggcag
     8401 ccaggtcacg ccacatctgc tgacgctgcc ggccggtgag cccaaccccg tgatgcggct
     8461 tcaccaggtt tcgtacgcgt tcaaggcgca caaggagacc ggccgggcgg tggcggcgga
     8521 caagctcgcc gagctgtccg gcttcgcacc gtcgacgttc cacgcgatcg gcgcacggct
     8581 cgccgccagc gaggcatcgc gcagcttcga tctcgtcgtc accaatgttc ccggtccgca
     8641 gttcccgctg tacgcagcgg gggctcggct gcaggcgagc tatcccgtac cgccgctgtt
     8701 ggcgggccag gcgctggcga tcggcgtgac gtcgtacaac ggcaaggtgt tctacggtct
     8761 gaacgccgat cgagatgcca tgcccgacat cgatgtcctg gcggcctgcg tgagcgatgc
     8821 gctcgacgag ctggtggacg cctcgtcgtc gaccagagca cgcgccccgc gcggaaaggt
     8881 gaagcgcgcg gcgacgaaga aggcaccacg accgaaggcc tcggccgggg aagggtcctg
     8941 atgcgggtct atgtcgccgc aacgatcgag cggctgcagt cctggcacga cgatcaggtg
     9001 ctgcaggctc cggttgctgc ctttgcggtg acggacgact tgcggacgga gctccccgat
     9061 ctcggggacg aggaggcgga gtacgccgtc gcgaccgcag cggccgaggc gtccgggcag
     9121 atgttgaccg acggcatgca caagcgtggc cggcgcgtgg ttgtcgtggt ggaggtgtcc
     9181 tactccgttg tggagcccga cgacgagacc ccgggcgcag tgaacgtcgt cgcgccgatc
     9241 gaggtgcagc acatcgcggc tgtgctcgcc gaccccgtcg acatccccct cactggcggc
     9301 ggcgagatgg acgacctcgc ctggttcgcc gtgcaggaga ttcccgaatt gctagtgtcg
     9361 aagcacgacg gcgaccagta gccgacgagg agggcacatg gcgcaggcga tctggaaggg
     9421 ctcgatctcg ttcggtctcg tcacgatccc ggtcaagctc tactcggcga ctgaggagaa
     9481 ggacatctcg ttccgccagg tgcatgagga ggacggcggg cggatccgct accgccgcgt
     9541 ctgcgagatc gacggcgagg aagtgccgta ctccgacatc ggcaagggct atgagatgcc
     9601 cgatggcagg atggtcatcc tcaccaacga cgactttgcc gatctgccgc tgccgacagc
     9661 gaagtccatc gaggtactcg agttcgtgcc gatcgagcag gtcgatccgc tctttttcgc
     9721 caaggcctac tatctcggtg ccgacggcgg tcccggcggc aagccgtacg tgctgctgcg
     9781 tgacgcgctc acgaagacca agcagtgcgc gttggtgaag gtcgcgatcc gcaccaagga
     9841 ggtgctcgct cttctgcgtg agaaggaggg cgccctggtg ctgcagacga tgttgtggcc
     9901 ggacgaggta cgtgacgccg gcttcgccgc gccggacgac tcgatcgaga tccgcaagca
     9961 ggaagtcgag atggccgagt cgtacatcgc aacgctgcgg gccgacttcg agcccgagaa
    10021 gttccacagc gactaccgcg aggcgctgga gaggctcgtg gaggcgaagg cggagggcgt
    10081 gccggtaccc gaggtcgagg aggacgagag cgaaccggcc gaggtcgtcg accttgtcgc
    10141 tgcgctgcgc gccagcgtcg acgcagccaa gcgcaggagg accgctcccg catctggcca
    10201 cgcccctgcc gaaagaggca gccctaccgc acggcaagcc ggcgccgagg catcggcgaa
    10261 gaagtcgcgc accaagaagt ccgacaagaa ggcggccggc aagaagaccc ccgccaagaa
    10321 atccgcagcg aagaaggctg ccgcaaagaa atctaccgcg aagaagtcga cgagtaagaa
    10381 gagccccgtc aagaagtccg cctgagcgcc ttcacgtcag gtgagcccgc gtggccagtg
    10441 ctgagttgcc agtgctgacg cggcccgtgc cgaagtggca aacacgtcac cggaaccggc
    10501 gtgtctccaa caaaacctgc cacctcggcg aggactccc
//
LOCUS       DATBFZ010000573         1612 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_6519889, whole genome shotgun sequence.
ACCESSION   DATBFZ010000573 DATBFZ010000000
VERSION     DATBFZ010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6519889"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..781
                     /locus_tag="VH419_17540"
     CDS             <1..781
                     /locus_tag="VH419_17540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7045478.1"
                     /translation="AATNPARGFWMRQDLAGICATWETAVPAERLHVITVPRSGASPD
                     ELLARFASVVGFAPKALTAEPRWNNETVGVAATEVIRQVNQRLGGRLNQHEHDMIIKM
                     TVVQLLAQRSESVRFSVPEDELGWIGDRAEHSIKELAERGYAIVGDLDELRPVHRDGS
                     RRPDDATESELLDAALDALAVLAEDYATAWWEAKKSSVEGVKEAGDVRSRTRRLVFEG
                     QRRAATLADSNPVAAKAMGAVLRRRERARERARAKTTKKSS"
     gene            complement(810..>1612)
                     /locus_tag="VH419_17545"
     CDS             complement(810..>1612)
                     /locus_tag="VH419_17545"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY7045479.1"
                     /translation="GGLTRLDRPNRRGGGMKKVDYDERLHAGYAAGRKLSPAALQTWM
                     DTFARHLPQTRPLAWLDLGSGTGRMAPALANAFGGPVHGVEPADKMRAQAVAHADHPA
                     VTYTAGSAESIPLPDDSCDAALLFFVWHHVVDRDSAAQELLRVVKPGGKLFVQANFSD
                     RMPDVWWFQVVPEWRQADTAQFRSEEDVTTDFVDAGWTLVSRDEVRWLRSATLTEDFE
                     RLKLRAVSVFAHLSEEAADEGFARIEAARPSLGDGPQYETSQLLVFER"
BASE COUNT          259 a          532 c          581 g          240 t
ORIGIN      
        1 ggcggcgacc aacccggccc gtggtttctg gatgcgacag gatctggccg ggatctgtgc
       61 gacgtgggag accgcggtgc cggccgagcg gttgcacgtc atcaccgtgc cccgttcggg
      121 cgcgtcaccg gacgagctgc tggcccggtt cgcgtccgtg gtcggcttcg cgccgaaggc
      181 gttgaccgcc gagccgcgct ggaacaacga gacggtgggc gtcgccgcga ccgaggtgat
      241 tcgacaggtc aaccagcgtc tgggcggtcg gctcaaccag cacgagcacg acatgatcat
      301 caagatgacc gtggtccagc tgctggcaca gcgctctgag tccgtgcggt tcagcgtccc
      361 cgaggacgag ctcggctgga tcggtgaccg cgccgaacac tccatcaaag agctggccga
      421 gcgtggctac gcgatcgttg gcgatctcga cgagctacga cccgtgcacc gggacggcag
      481 tcggcgtccc gacgatgcga ctgagtcgga gctgctcgac gcggcgctgg atgcgctcgc
      541 cgtgctggca gaggactacg ccactgcctg gtgggaggcg aagaagtcgt cggtcgaggg
      601 ggtcaaggag gcgggggacg tccgcagccg gacccgacgc ctggtcttcg agggacagcg
      661 ccgggcggcg accctcgccg acagcaaccc ggtggcagcc aaggcgatgg gcgccgtgct
      721 ccggcgtcgc gagcgggcgc gagagcgggc ccgggccaag acgacgaaga agtcctcctg
      781 acgacaccga ggtgcggaag ccggcttggc tagcgctcga agacgagcag ctggctggtc
      841 tcgtactgag gtccgtcgcc gagtgagggt cgagctgcct cgatcctggc gaagccctcg
      901 tcggccgctt cctcgctgag atgcgcgaag accgagacgg cccgcagctt cagcctctcg
      961 aagtcctcgg tcagggtcgc tgaccgaagc cacctgactt catcacggga gacgagcgtc
     1021 cacccagcgt cgacgaagtc cgtcgtcacg tcttcttcgg accggaactg cgccgtgtcg
     1081 gcctgcctcc actcggggac gacctggaac caccagacgt cgggcatccg gtcggagaag
     1141 ttcgcctgga cgaagagctt gccccctggc ttgacgaccc ggagcaactc ctgagcagcg
     1201 ctgtcgcggt cgacgacgtg gtgccagacg aagaacagca aggctgcgtc gcacgagtca
     1261 tccggcaacg ggatgctctc ggcggaccca gcggtatacg tgacggcggg gtggtcggcg
     1321 tgtgcaacgg cttgcgcgcg catcttgtcc gcgggctcca cgccgtggac cgggccgccg
     1381 aacgcattcg ccagggcggg ggccatccgt cccgtgccgg agcccaggtc gagccaggcc
     1441 agcggccgcg tctgcggcag gtggcgagcg aacgtgtcca tccaggtctg cagcgcggcg
     1501 ggcgacagct tccggccggc ggcgtaaccg gcgtgcagac gttcgtcgta gtcgaccttc
     1561 ttcattccac ctccacgtcg gttcggccgg tccagcctag tcaggccgcc gg
//
LOCUS       DATBFZ010000574         8415 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_6525770, whole genome shotgun sequence.
ACCESSION   DATBFZ010000574 DATBFZ010000000
VERSION     DATBFZ010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 8415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8415
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6525770"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..991)
                     /locus_tag="VH419_17550"
     CDS             complement(<1..991)
                     /locus_tag="VH419_17550"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEY7045480.1"
                     /translation="MSTLAIVLAAGQGTRMQSERPKPLHVICGRPMVVHVLHALEGLD
                     IDRTAVVVGHDAPRVVKRVQADRPAGLELTFVEQRVQRGTGDAVMVGLIAFADDDLDD
                     TSTVLIIPGDTPLLRSQTLADLIAFHEDSGHGATLLATHMADPTGYGRLVRNKHGLVA
                     RVVEEADATPQERAIQEVNTSIYCFRRDLLGPALRRVSPDNSQAEYYLTDVVAVLHDT
                     GHRVDALTLDDAQEVQGVNDRWQLAIAERELRARINHAWLLAGVTMLDPRQTFIDATV
                     SLGRDVTLYPGTMLQGGTVIGDGCEIGPDTRLVDSVVGDGAVVENAVVRESHIG"
     gene            complement(1201..1983)
                     /locus_tag="VH419_17555"
     CDS             complement(1201..1983)
                     /locus_tag="VH419_17555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7045481.1"
                     /translation="MSSTETGGRLVDERLVELLSLVNRSDSVEMKLTVPEAAHRSTVV
                     GLGMDPLAAQIRQVFFFDTPDLALNRAGVVARARRIQAKGGDSVIKLRPVVPDELPED
                     LRRSPGMVVEVDAMPNGFVCSASLKGRVEDQMILDAAAGRQPIRKVFTKEQRAFYAAH
                     APEGLELDSLAVLGPIFVLKLNFVPEGWDGRMVAEMWFYPDGSQILELSTKSLPADAL
                     HVGLALRSFLRDKGVEVTDEQQTKTKTALEFFSSQLQSAANP"
     gene            complement(2088..2288)
                     /locus_tag="VH419_17560"
     CDS             complement(2088..2288)
                     /locus_tag="VH419_17560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7045482.1"
                     /translation="MGKPAEPPMSGSTFVLLTLLIVLAGYLLVRWILGTIALVFNTAV
                     LIAVLAGAVYLYVRFRESRSNR"
     gene            2502..4913
                     /locus_tag="VH419_17565"
     CDS             2502..4913
                     /locus_tag="VH419_17565"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEY7045483.1"
                     /translation="MKTGEVVAQPSASPTGPVAAATTVAPVSAASPEAARAAVVDLPE
                     SRSYRLKKRLLGPPLHTERLAHERLGKPTALAVFASDNLSSSAYATEEILRVAIPAVG
                     VAAFSLVVPITVALLIVLGFLILSYRQTIKAYPSAGGAYIVTRDNFGLLPAQVAGVAL
                     LTDYVLTVSVSVAAGIAALSSAFPALSPYNLWLSIFCVLLIAGGNLKGVRESGKIFAV
                     PTYFFIANMAILLSVGVARMSWGHLPVDSLDHAGLVKVGSPAVGLFYGASLYVVLHAF
                     ASGGAAVTGVEAISNGVPAFREPAWKNARTTLVIMGSLLGVMFLGLSTLAAKVHAAPY
                     ESGTPTVISQVGKLVYGPSASGHLLYYMLQAGTMLILILAANTSFADFPRLASFHAGD
                     NFMPRQLTKRGHRLVFSNGIIFLAAAGILLLLVTDAKVDRLIPLYALGVFTSFTLSQA
                     GMAKHHVTHKEQGWRFGLFVNGMGAGLSFLVAVIIAITKFTHGAWVVVVLVPVMVAGL
                     VRLNRQYEGEAEELEHDAPKAATVPILRRHVVLLFVDDLNLAAARALQYARTLTPDEL
                     RVIHFDLDPIRTEDLTGAWRRLGLSRLSLEIHELPDRRLNRAVLELVAAELTDGDTEV
                     SVLVPRIQHTRIWHRLIHDRTAATIAEALANLPHCNVTFVPYHLRSGAPKRTVEFIAK
                     HEPPPNGNARHQSASISADAGLPRVAGSIPIGEIPPRRKVQVTGRVVAVRVQPWGGAP
                     TLEATLRDATGEVTLVFLGRRAVGGVGPGTVLTAEGVLGKHHNHSALLNPSYTLLSVP
                     GNGHH"
     gene            complement(4956..5708)
                     /locus_tag="VH419_17570"
     CDS             complement(4956..5708)
                     /locus_tag="VH419_17570"
                     /inference="COORDINATES: protein motif:HMM:NF024884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HEY7045484.1"
                     /translation="MSGRRSPGWGSWLTRIALATAISIGLVAVLIPLRTHTRSANLAL
                     VLVLAVLAAAVVGGRVIGVVTGVVIAVAFDFFLTQPYGSFSIERGDDVQTTILLAAVG
                     LVGGELVERARRSEADAEARRREVARFQRRAELAASGEPPARLISRSAEELAELLSAV
                     DVQYRAGAAPARVAVLTHDGARVPAGPDAVGADVVALPVRAHGRDIGHFLLLFSRAHA
                     GMSVPVDQRHAAVAVADQLGLALLRYDRQRPA"
     gene            5994..6467
                     /locus_tag="VH419_17575"
     CDS             5994..6467
                     /locus_tag="VH419_17575"
                     /inference="COORDINATES: protein motif:HMM:NF018663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY7045485.1"
                     /translation="MPQITPNLWFDLESKDAAEFYVSVFPNSKITNVTYYNAAGPRPA
                     GMILTVEFELDGQPYIAINGGPEFTFSEAISLLVNCADQAEIDYYWDKLSEGGEEGPC
                     GWLKDKFGLSWQVAPLGMDEMLNDPDSERGARAMKAMFGMKKIDLAALQAAADGA"
     gene            6645..8231
                     /locus_tag="VH419_17580"
     CDS             6645..8231
                     /locus_tag="VH419_17580"
                     /inference="COORDINATES: protein motif:HMM:NF042648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N,N-dimethylformamidase beta subunit family
                     domain-containing protein"
                     /protein_id="HEY7045486.1"
                     /translation="MSANPPVAGSAPELKRPFTMWSAVEGFTDRQSYRSGETVEVRCS
                     GRPSSMTAVVSRVGRERAEVWRQSGIGVSEQPVADDAYATGCDWPVTFTLEVDASWPS
                     GFYEVSLQADGVGGDQARSEAFFVVRSSGRSAADALLVLATNTYNAYNQWGGKCLYSG
                     ATSVSFARPIERGYLRRPASPDDAAYDGRVASITEEPDEEHHQFQDYLRNGPYPLWCA
                     SSGWHNWERRFVRWAESEGISLDYAVNADLQLHPEVLDGHRLMASVGHDEYWSWAMRD
                     RADTFVEDGGGWAIFSGNTCFWQVRIEDDGRTMVCFKSRFAEDPVLGTDRAQLLSTMW
                     SLPAIGRPEAETTGLSFSRGGYARVGKATPRSSGGYTVHRPDHPVFAGTDLRYGDVLG
                     AADRIVGYEVDGCELTVVNGDPVPTYNDSTPAGFEVLGTAPARLLSVTTTTSEIPPAL
                     WANTDPPGDLEGVAAALFGSASPENVARIAHNHAVMGTFEKGRGRVVHTGTTDWAFGL
                     DRDPLVQQVTGNILRWLAGR"
BASE COUNT         1279 a         2912 c         2918 g         1306 t
ORIGIN      
        1 agccgatgtg gctctcgcgc acgacggcgt tctcgaccac ggcgccgtca ccgacgacgc
       61 tgtcgaccag gcgggtgtcc ggtccgatct cgcagccgtc gccgatgacg gtgccgccct
      121 gcagcatcgt gcctggatag agcgtgacgt cgcgaccgag gctgacggtg gcgtcgatga
      181 acgtctggcg cgggtcgagc atggtgaccc cggccaggag ccaggcgtgg ttgatgcgcg
      241 cccgcagctc gcgctcggcg atcgcgagct gccagcggtc gttgacccct tgcacctcct
      301 gcgcgtcgtc gagcgtgagc gcgtcgacgc ggtggccggt gtcgtggagc acggcgacga
      361 cgtcggtgag gtagtactcg gcctggctgt tgtccggtga gacacgacgc agggccgggc
      421 ccagcaggtc tcgccggaag cagtagatgg aggtgttgac ctcctggatg gcccgctcct
      481 gcggcgtcgc atcggcctcc tcgacgacgc gcgccaccag cccgtgcttg ttgcgcacga
      541 gccggccgta gccggtgggg tcggccatgt gcgtggcgag gagcgtcgcg ccgtggccgc
      601 tgtcctcgtg gaaggcgatg aggtcggcga gggtctgtga gcggagcagc ggcgtgtcgc
      661 ccgggatgat cagcacggtg gaggtgtcgt cgaggtcgtc gtcggcgaag gcgatgagcc
      721 ccaccatcac ggcgtcgccg gtgccccgtt gcacccgctg ttcgacgaag gtgagctcga
      781 gacccgccgg acggtcggcc tggacccgct tgaccacccg gggggcgtcg tggcccacca
      841 ccaccgcggt ccggtcgatg tcgaggccct ccaaggcgtg caggacgtgg accaccatcg
      901 gccggccgca gatgacgtgc aggggcttgg gtcgctcgga ctgcatgcgc gtcccctggc
      961 cggcggcgag gacgatcgcc agggtgctca catcgtcata tctagcgccc tcagggcacc
     1021 gatcacatgc gcggcgcagt cttgctggtg tcgagctggg acagcaggac gactgggacg
     1081 cgccgatcgt ggtggtcggc ctgcgctgcg ccgctgagcg cggtgttacc ctccgcgccg
     1141 ggagggcctg catggggagg cctgacatgg gggaagcccg tgacgggagg gcctcgctcg
     1201 tcaggggttg gcggccgact gcagctgcga cgagaagaac tcgagggcgg tcttcgtctt
     1261 cgtctgctgt tcgtccgtga cctcgacgcc cttgtctcgc aggaaggatc gcagtgcgag
     1321 gccgacgtgg agcgcgtcag cgggcaggct cttcgtcgag agctcgagga tctggctgcc
     1381 gtccgggtag aaccacatct ccgccaccat ccgcccgtcc cagccctcgg gcacgaagtt
     1441 gagcttgagc acgaagatcg gtccgaggac cgccaggctg tcgagctcga gcccctccgg
     1501 agcgtgcgcg gcgtagaagg cgcgctgttc cttggtgaac accttgcgga tgggctgccg
     1561 accggcggcc gcgtcgagga tcatctggtc ctcgaccctg cccttgagcg acgccgagca
     1621 gacgaagccg ttgggcatcg catcgacctc gacgaccatg cccggcgagc ggcgcaggtc
     1681 ctctggcagt tcgtcgggta cgacggggcg aagcttgatc accgaatccc cgcccttggc
     1741 ctggatccgc cgggcgcggg ccacgacgcc ggcccggttc agcgcgaggt cgggcgtgtc
     1801 gaagaagaag acctgcctga tctgcgccgc gagtgggtcc atgccgagcc cgacgacggt
     1861 cgagcggtgc gcggcctcgg gcaccgtgag cttcatctcg acgctgtcgg agcggttgac
     1921 gagcgacagc agctcgacga gccgctcatc caccagacgt ccaccggttt ccgtggagct
     1981 catgggtgcc ctccctgatc ttcgtgcctg cgtacgcaac ggcgcacggt ccgtgatcat
     2041 ggcacttccg cgacgcccgg ttcaggtgac gcgtcagccg aacgagctca gcggttgctc
     2101 ctgctctctc ggaagcgaac gtacaggtac accgcgccgg cgagcacggc gatcaacacc
     2161 gcggtgttga acacgagtgc gatggtcccc aggatccacc ggaccaggag gtagccggcc
     2221 agcacgatca acagcgtcag caggacgaac gttgaacccg acatcggggg ctctgcgggc
     2281 ttgcccatgt tcctgctcct gcctcgtcgc tcgctccgtg cggctccgga cgtccgaacc
     2341 gtagaccgac agctgggcga cgtcgtcgcg gctcgcgccg tcagtcgacc gggcccgagc
     2401 gccatccaaa cgcctccttg acgcgtccgc gcccgatctt gtcgggatct tgacgccgca
     2461 caacggactc accgcagctc accggtgttg actcggaagc ggtgaagaca ggcgaggtcg
     2521 tcgcccagcc gagcgcgagc ccgaccggac cggtagcggc cgcgacgacc gtcgctccgg
     2581 tgtcggcggc gtcgccggag gcggcgcgag ccgcggtcgt cgacctcccg gagtcgcgta
     2641 gctaccgcct caagaagcgt ctgctcggac cgccgctgca caccgagcgt ctcgcccacg
     2701 aacgactggg caagccgacc gccctcgccg tgttcgccag cgacaacctg tcgtcgtcgg
     2761 cctacgccac ggaggagatc ctccgggtcg ccatcccagc ggtcggcgtg gccgcgttca
     2821 gcttggtcgt accgatcacc gtcgcgttgt tgatcgtcct tggcttcctc atcctctcgt
     2881 accgccagac gatcaaggcc tacccgagcg cgggcggcgc gtacatcgtg acgcgcgaca
     2941 acttcgggct ccttcccgca caggtcgccg gtgtcgccct tctcaccgac tacgtgctga
     3001 cggtgtcggt ctccgtcgcc gcaggcatcg ccgcgttgtc gtctgctttt ccggcgctgt
     3061 ctccgtacaa cctgtggctc tcgatcttct gcgtgctgtt gatcgccggc ggcaacctga
     3121 agggcgtcag ggagtcaggc aagatcttcg ccgtccccac ctacttcttc atcgccaaca
     3181 tggcgatcct gctctcggtc ggcgtggccc ggatgtcctg ggggcacctg cccgtcgact
     3241 cgctcgacca cgcgggcctg gtgaaggtgg gctcgccggc cgtcggtctg ttctacggcg
     3301 cgtcgctgta cgtcgtgctg cacgcgttcg cgtcgggagg cgcggcggtc acgggcgtcg
     3361 aggcgatctc gaacggtgtg ccggcgttcc gcgagcccgc atggaagaac gcccgcacga
     3421 ccctcgtgat catggggtcg ctcctcggcg tcatgttcct cgggctgtcg acgctcgccg
     3481 cgaaggtcca tgccgcgccg tacgagtcgg ggacgccgac ggtcatctcg caggtgggca
     3541 agctcgtcta cgggccttcg gcgagcgggc acctgctcta ctacatgctc caagcgggca
     3601 cgatgctcat cctgatcctg gccgcgaaca cgagcttcgc cgacttcccc cggctcgcca
     3661 gcttccacgc cggtgacaac ttcatgcccc gtcagctcac caagcggggt caccgactgg
     3721 tgttctcgaa cgggatcatc ttcctcgccg ctgccggcat cctcctgctg ctcgtcaccg
     3781 acgcgaaggt ggaccggctg atcccgctct acgccctggg cgtgttcacg agcttcacgt
     3841 tgtcacaggc gggcatggcc aagcaccacg tcacccacaa ggagcagggc tggcggttcg
     3901 gcctgttcgt caacggcatg ggcgcggggc tgtcgttcct cgtcgccgtg atcattgcga
     3961 tcaccaagtt cacccacggc gcctgggtcg tcgtcgtcct cgtcccggtc atggtggcgg
     4021 ggctggtgcg cttgaaccgg cagtacgagg gggaggcgga agagctcgaa cacgacgcac
     4081 caaaggcggc gacggtcccg atcctgcgcc ggcacgtcgt cctcctgttc gtggatgacc
     4141 tcaatctggc cgcggcgcgt gccctgcagt acgcccggac gctcaccccg gacgagctgc
     4201 gagtgatcca cttcgacctc gacccgatcc gcaccgagga tctgaccggc gcatggcgtc
     4261 gtctcggcct ctcgcgcctg tcgctcgaga tccacgagct gcccgatcgg cggctgaacc
     4321 gcgccgtcct cgagctcgtg gcggccgagc tgaccgatgg cgacaccgag gtctcggtcc
     4381 tggtgccgcg gatccagcac acccgcatct ggcaccgtct catccacgac cgcaccgcag
     4441 cgacgatcgc cgaagcgctc gccaacctcc cgcactgcaa cgtgacgttc gtgccgtatc
     4501 acctgcgctc cggtgctccg aagcgcacgg tcgagttcat cgccaagcac gagccgccgc
     4561 ccaacggcaa cgcccgccac cagtcggcgt cgatctcggc cgacgctggc cttccccgcg
     4621 tggccgggtc gatccccatc ggtgagatcc caccccgccg caaggtccag gtgaccggcc
     4681 gcgtggtggc ggtgcgggtc cagccgtggg gtggtgctcc cacgctcgaa gcgacgctgc
     4741 gcgacgccac gggcgaggtg accttggtgt tcctcgggcg acgggcggtc ggcggggtcg
     4801 ggccgggcac ggtgctgacc gccgagggtg ttctcggcaa gcaccacaac cactcggcgc
     4861 tgctcaaccc gagctacacg ctgctgtcgg tcccgggcaa cggccaccac tgacgccgcc
     4921 gcggtgctgg ctgcggatcg tgcgttcctc cgtcgtcagg ctggtcgctg ccggtcgtag
     4981 cggagcaggg ccaggccgag ctggtcggcc accgccaccg ccgcgtgtcg ctgatccacg
     5041 ggcaccgaca tgccggcgtg ggcgcgggag aacaacagga ggaagtgccc gatgtcgcgg
     5101 ccgtgggccc gcacggggag ggcgacgacg tcggcgccga cagcatcagg acccgccggc
     5161 acccgcgccc cgtcgtgggt gagcaccgcc acccgcgccg gcgcggctcc agcgcggtac
     5221 tgcacatcga ccgcgctcag cagctccgcc agctcctcgg cggagcggga gatgagacga
     5281 gccggtggct cgccggacgc ggccagctcg gctcgacgct gaaaccgagc gacctcgcgc
     5341 cggcgggctt cggcgtcggc ctcactgcgc cgtgctcgct caacgagctc accgcccacc
     5401 agtcctactg cggcgagcag gatcgtcgtc tggacatcgt cgccccgctc gatgctgaac
     5461 gacccgtacg gctgggtcag gaagaagtcg aacgccacgg cgatcacgac gccggtgacg
     5521 accccgatga cgcgaccgcc gacgacggcg gcggcgagga cggcgaggac gaggacgagc
     5581 gcgaggttcg cgctccgggt gtgcgtccgc agggggatga ggacggccac caacccgatc
     5641 gagatggcgg tggccaatgc gatccgcgtc agccacgatc cccacccagg ggagcgccga
     5701 ccagacacag cagccgtcta cgaccgacgc catgccgagt caagcgccct aacgcgatct
     5761 tgacgctgcc gtgtccgcgg gaagtcgacc ggaccggatc gcccgtaccg gatcgcccgt
     5821 accgccaacc ccgacggaag cgtcaaatcg acgttcatgt cgcggaaaag cgcgatccgc
     5881 cgtcgagatg cgcgacatga acgctggccg ggcactccgg tgcgcgagtg gcgatgagtc
     5941 tcggcgggat cccgagtcat catccagagc cgtactgacg tcaggaggat gtgatgccgc
     6001 agatcacccc gaacctctgg ttcgacctgg agagcaagga cgccgccgag ttctacgtct
     6061 ccgtgttccc gaactccaag atcacgaacg tcacctatta caacgccgcc gggccgcggc
     6121 cggcgggaat gatcctcacc gtggagttcg agctcgacgg ccagccgtac atcgcgatca
     6181 acggcggtcc ggagttcacg ttcagcgagg cgatctcgct cctggtcaac tgcgccgacc
     6241 aggccgagat cgactactac tgggacaagc tctcggaggg cggcgaggag ggcccgtgcg
     6301 gctggctcaa ggacaagttc gggctgtcgt ggcaggtcgc gccgctgggc atggacgaga
     6361 tgctgaacga ccctgactcc gagcgtggcg cgcgtgcgat gaaggcgatg ttcggcatga
     6421 agaagatcga cttggccgcc ctgcaagccg cggcggacgg cgcctgattc gacccgcctg
     6481 agcgaggact cctccgggtc gccctttcat ctcttgtgga tgacgaccgg cccaacgatc
     6541 gcgtggagaa tggccgagcg gatccaccgt cgtgaccgcg ctgacgcggc gcgggctgag
     6601 gttcgaggag aggggaccgt gggcgtgttg aaggggccga ccgaatgagc gccaacccgc
     6661 cggtcgctgg atcggctccg gagctgaagc ggcccttcac gatgtggtcg gcggtcgagg
     6721 gcttcaccga ccgccagagc taccggtcgg gggagacggt cgaggtgcgc tgcagcggcc
     6781 ggccgtcgtc gatgaccgcc gtcgtctcgc gcgtcggccg cgagcgcgcc gaggtgtggc
     6841 gtcagagcgg catcggcgtc agtgagcagc cggtggccga cgacgcgtac gcgaccgggt
     6901 gcgactggcc ggtcacgttc acgctcgagg tcgacgcatc gtggccgtcg ggcttctacg
     6961 aggtcagcct gcaggccgac ggcgtcggcg gcgaccaggc ccggtccgag gccttcttcg
     7021 tggtccgttc cagtggtcgg tcggcggccg acgcgctcct cgtcttggcc acgaacacgt
     7081 acaacgccta caaccagtgg ggcgggaagt gcctctacag cggggccacc tccgtctcgt
     7141 tcgcccgacc catcgaacgc gggtacctcc gccgtccagc gtcaccggac gacgccgcct
     7201 acgacggccg ggtggcgagc atcaccgagg agcccgacga agagcaccac cagttccagg
     7261 actacctccg caacggcccg tacccgctgt ggtgcgcgtc gtcgggatgg cacaactggg
     7321 aacggcgctt cgtccgttgg gccgaatcgg aaggcatcag ccttgactac gcggtgaacg
     7381 cagatctcca actgcatccc gaggtgctcg acggccatcg gctcatggcg agcgtcggtc
     7441 acgacgagta ctggtcgtgg gcgatgcgcg accgtgccga caccttcgtc gaggatggcg
     7501 gcgggtgggc catcttcagc ggcaacacgt gcttctggca ggtgcgcatc gaagacgacg
     7561 gtcgaacgat ggtctgcttc aagagccgct ttgccgaaga tccggtgctc ggcaccgatc
     7621 gggcccagct cctgagcacc atgtggtcgt tgccggcgat cggccgtccc gaggcggaga
     7681 cgacggggct cagcttcagt cgcggcggct acgcgcgcgt ggggaaggcc acgccgcgca
     7741 gctcgggtgg ctacacggtg caccggccgg accatccggt gttcgccggc accgacttac
     7801 gctacggcga cgtgctgggc gcggcggacc ggatcgtcgg ctacgaggtc gacggctgcg
     7861 agctgacagt ggtgaacggc gacccggtgc cgacctacaa cgactcgacc cctgccggct
     7921 tcgaggtgct gggcaccgcg ccggcccgct tgctctcggt caccacgacg acgagcgaga
     7981 tcccaccagc gttgtgggcc aacaccgatc cgcctggcga cctcgagggt gtggccgcgg
     8041 cgctgttcgg atcggcgtcg ccggagaacg tcgcccgcat cgcccacaac cacgcggtga
     8101 tggggacgtt cgagaagggc cggggccggg tcgttcacac cggaacgacc gattgggcct
     8161 tcggtctgga ccgcgacccg ctcgtgcagc aggtgaccgg caacatcctc cgctggctgg
     8221 ccggtcgctg accgccgagg gctcggcgcc cgcagtcgcc gggcgcggcg ttcactcgct
     8281 gcgcaggacc tccgccggtc gcagacgact ggcccgacga ccgggccacc acgcgagcgc
     8341 gaccgcggcg gcgacgatca ccgccgcgaa cagcacgaga ccggccgccg acacgtcggt
     8401 acgcggcacg agatg
//
LOCUS       DATBFZ010000575         2429 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_5872255, whole genome shotgun sequence.
ACCESSION   DATBFZ010000575 DATBFZ010000000
VERSION     DATBFZ010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5872255"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1922)
                     /gene="glgB"
                     /locus_tag="VH419_17585"
     CDS             complement(<1..1922)
                     /gene="glgB"
                     /locus_tag="VH419_17585"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017935547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEY7045487.1"
                     /translation="MTVSSIGSAELERLVGGAHHDPHGVLGAHPTGDGRTVIRTLRPD
                     ATSVDVVIGKSTAPLGLIHDGGVWEAIIDGEPPDYRLDVAYGSDHYVVDDPYRWLPTL
                     GDIDLHLIGEGRHENLWEVLGAHVHTYDTPGGPVTGTSFAVWAPNARGVRIAGDFNFW
                     MGHALPMRSLGSSGVWELFVPGVGDGDRYKFRVLGADSQWREKADPMAFATEVPPATA
                     SVVHTDRYTWADDEWLARRAETNWVAAPMSIYEVHLGSWKFGMTYRELANDLVNYVRD
                     AGFTHVEFLPVAEHPFGGSWGYQVSSYYAPSARFGNPDDFRYLVDALHGAGIGVIVDW
                     VPAHFPKDAWALGRFDGTPLYEHGDPRRGEQPDWGTFVFDFGRREVRNFLVANALYWL
                     EEFHVDGLRVDAVASMLYLDYSRAEGQWVPNVHGGRENLDAVSFLQEMNATVYKRVPG
                     VVTVAEESTSWPGVTRATHLGGLGFGFKWNMGWMHDTLSYVSRAPIHRQYHHNDMTFS
                     MMYAYSENFVLPISHDEVVHGKGSMLNKIPGDRWQQMATLRALYAYMWAHPGKQLLFM
                     GQEFGQGHEWAESRSLDWWLLDGPDHRGVARLVTDLNRVYRETPALWSQDNDPKGFHW
                     IDANDASGNVYSFVRFG"
     gene            complement(1919..>2429)
                     /locus_tag="VH419_17590"
     CDS             complement(1919..>2429)
                     /locus_tag="VH419_17590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase"
                     /protein_id="HEY7045488.1"
                     /translation="ELEPLAAGLRATYDAFAEAASEVSLQRIHGDLHLGQALRTSHRW
                     VLIDFEGEPMADLDDRRRPDSTLRDIAGMLRSFEYAGHHRVVEAGYQPQLSYRASEWT
                     QRNRDAFLAGYAEAADHDPREHEVPLRAFEADKAVYEAVYEARNRPAWLPIPLATLSR
                     LAAAESETS"
BASE COUNT          376 a          837 c          848 g          368 t
ORIGIN      
        1 ccgaaccgga cgaaggagta cacgttcccc gacgcgtcat tggcgtcgat ccagtggaag
       61 cccttcgggt cgttgtcctg cgaccacagg gcgggggtct cgcggtagac ccggttgaga
      121 tcggtgacca gccgggccac gccgcggtgg tccggcccgt cgagcagcca ccagtccagc
      181 gaccgcgact cggcccactc gtgcccttgc ccgaactcct ggcccatgaa cagcaactgc
      241 ttgccgggat gggcccacat gtaggcgtag agcgcgcgca gcgtcgccat ctgctgccag
      301 cggtcaccag ggatcttgtt cagcatcgac cccttgccgt ggacgacctc gtcgtgcgag
      361 atcggcagca cgaagttctc ggagtaggcg tacatcatcg agaacgtcat gtcgttgtgg
      421 tggtactgac gatggatcgg cgcccgcgag acgtaggaca gcgtgtcgtg catccagccc
      481 atgttccatt tgaaaccgaa gcccaggccg ccgagatgcg tcgcgcgggt gacgcccggc
      541 catgacgtcg actcctcggc gacggtcacc acgccgggga cccgcttgta gaccgtggcg
      601 ttcatctcct gcaggaacga caccgcgtcg aggttctcgc ggccgccgtg gacgttgggc
      661 acccactgac cctccgcacg cgagtagtcg aggtacagca tcgacgccac ggcgtcgacg
      721 cgcagcccgt ccacgtggaa ctcctcgagc cagtacagcg cgttggcgac caggaagttg
      781 cgtacctcgc gcctcccgaa gtcgaacacg aaggtgcccc agtcgggctg ctcgccacgg
      841 cgtgggtcgc cgtgctcgta cagcggtgtc ccgtcgaagc ggccgagcgc ccacgcgtcc
      901 ttcgggaagt gtgcgggcac ccagtcgacg atcacgccga tgccggcgcc gtgcagcgcg
      961 tcgacgaggt agcggaagtc gtccgggttg ccgaagcgcg ccgacggcgc gtagtaggac
     1021 gacacctggt agccccagga accgccgaac gggtgctcgg ccaccggcag gaactcgacg
     1081 tgggtgaacc cggcgtcgcg cacgtagttg acgaggtcgt tcgcgagctc gcggtaggtc
     1141 atgccgaact tccacgaacc gaggtgcacc tcgtagatcg acatcggcgc ggccacccag
     1201 ttcgtctcgg cgcgccgggc cagccactcg tcgtcggccc aggtgtagcg atcggtgtgc
     1261 accaccgagg ccgtcgccgg cggcacctcg gtggcgaagg ccatcggatc ggccttctcg
     1321 cgccactggc tgtccgcacc gagcacgcgg aacttgtacc ggtcaccgtc gccgacgccg
     1381 ggcacgaaca gctcccacac accggacgag ccgagcgagc gcatcggcag cgcgtggccc
     1441 atccagaagt tgaagtcgcc ggcgatgcgc acgccgcgcg cgttgggcgc ccacaccgcg
     1501 aacgaggttc cggtcaccgg gccaccgggc gtgtcgtagg tgtgcacgtg cgcgccgagg
     1561 acctcccaca ggttctcgtg ccggccttca ccgatcaggt gcaggtcgat gtcgccgagc
     1621 gtgggcagcc agcggtacgg gtcgtcgacg acgtagtggt cgctgccgta ggcgacgtcg
     1681 agccggtagt ccggcggctc gccgtcgatg atcgcctccc acacgccgcc gtcgtggata
     1741 agacccagcg gcgcggtgct cttcccgatc acgacgtcga cgctcgtggc gtcgggccgc
     1801 agggtgcgga tgacggtgcg gccgtcgccc gtgggatggg cgccgaggac gccgtgcggg
     1861 tcgtggtgcg cgccaccgac gaggcgttcc agttcggccg aaccgatcga gctcaccgtc
     1921 atgacgtctc gctctctgcc gccgcgagcc gcgacagcgt cgcgagcggg atcggcagcc
     1981 aggccgggcg gttgcgcgct tcgtacacgg cctcgtagac cgccttgtcc gcctcgaacg
     2041 cgcgcagcgg gacttcgtgc tcgcgcgggt cgtggtcggc cgcttcggcg tagccggcga
     2101 ggaaagcgtc gcggttgcgc tgcgtccact ccgatgcgcg gtaggacaac tgcggctgat
     2161 acccggcctc gaccacgcgg tggtgcccgg cgtactcgaa cgagcgcagc attccggcga
     2221 tgtcgcgcag cgtcgagtcc ggccggcgcc gatcgtcgag gtcggccatc ggctcgccct
     2281 cgaagtcgat gagcacccac cggtgcgagg tgcgcaacgc ctggcccagg tgcaggtcac
     2341 cgtggatgcg ttgcagcgag acctcggacg cggcctcggc gaacgcgtcg taggtggcgc
     2401 gcaaaccggc ggcgagcggc tccagttcc
//
LOCUS       DATBFZ010000576         4066 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_5875166, whole genome shotgun sequence.
ACCESSION   DATBFZ010000576 DATBFZ010000000
VERSION     DATBFZ010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 4066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4066
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5875166"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1399)
                     /locus_tag="VH419_17595"
     CDS             complement(<1..1399)
                     /locus_tag="VH419_17595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY7045489.1"
                     /translation="MNDTHDNRPSEMAESGADWRSWNPSRRRFLTLTGLAVGGTVIGV
                     SAAGCGTAQTGSQQGSGEQKGRPGASGETLFVAGFQWGPPKSFNPLAASPDFPVANGQ
                     PQYIYESLLRFNLLDGSLQPGLAKTVDMKDDTTMSVPLQDGTKWQDGSDLTADDVVFT
                     FELGKTASVNFSTVWQYIDSITAVDPKTVEFKLKTKPYNPNMVKNYLSTVLIVPKAVF
                     SKISPDKIPSETNLQPVGSGPFKLDKYDQTQVNLTRDDNYWGKSVYGTPAPATINHPI
                     FKSNNDGDVKLQSGEIDASQQFTAQIWKMWEAGKPVSTWSKKKPYHVPGQIPLLIFNL
                     NKKGLENVKVRQAIAYAIDYPNIATTAMSDYSVPANASLIIPSGYESKYYDGDAVKAD
                     GWTYDKDKAVSILEDDLKAKKGSDGIYVLPDGTKLGGWKLVTPTGWTDWNTACQIVAK
                     SATDIGIGITTEFPQF"
     gene            complement(2043..2573)
                     /locus_tag="VH419_17600"
     CDS             complement(2043..2573)
                     /locus_tag="VH419_17600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015071627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEY7045490.1"
                     /translation="MSDTLEKPATEHAAHPPFQASQQLADHLQQLHVDLIDLHLQGKQ
                     AHWNVVGKNFRDLHLQLDELVDAAREFSDTVAERMRAIYVTPDGRAATVAAKTTLAPF
                     PADEVDTGETVDLISTAIYAVAGTARRIHDDVDAEDPTTADILHAILERLEQLAWMID
                     AENRIANRSIPRPIHE"
     gene            complement(2945..3436)
                     /locus_tag="VH419_17605"
     CDS             complement(2945..3436)
                     /locus_tag="VH419_17605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008357673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY7045491.1"
                     /translation="MDQQSTEPSWLDADERAAWLGLLGVTLLLPSLLDSQLQRDSGLT
                     TFDYLVLAMLSETPDRTLQLKTLARLSNGSLSRLSHTVTRLQKRGWIRRETSPTDGRA
                     TVAVLTDEGYAKVVETAPGHVALVRRVVFDSVRPEDVPQLGRSMGAILATIDPTWVAT
                     PTA"
     gene            complement(3515..>4066)
                     /locus_tag="VH419_17610"
     CDS             complement(3515..>4066)
                     /locus_tag="VH419_17610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012918734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HEY7045492.1"
                     /translation="TKKIAFLVAAEGIEKAELVEPWDAVADAGHQPVLLSPKTGDVQL
                     FEHLDKADTRTVDAAVGEAQVADYDALVLPGGVANPDALRMDDAAVAFVKEFVESGKP
                     VASICHAAWTLVEADVVRGKQLTSWPSIRTDVRNAGGNWVDQEVVVDGNLITSRKPAD
                     LPAFNSALLEAVQHGVSSAAAVR"
BASE COUNT          613 a         1268 c         1394 g          791 t
ORIGIN      
        1 ggaactgcgg gaactcggtg gtgatgccga tgccgatgtc cgtggccgac ttggcgacga
       61 tctggcaggc cgtgttccag tccgtccagc cggttggcgt gacgagcttc cagccaccga
      121 gcttggtgcc gtcgggcagc acgtagatac cgtcggagcc cttcttggcc ttgagatcgt
      181 cctcgaggat ggagacggct ttgtccttgt cgtacgtcca gccgtccgcc ttcacagcgt
      241 cgccgtcgta gtacttggac tcgtacccgc tcgggatgat caggctcgcg ttggccggca
      301 ccgagtagtc cgacatcgcg gtggtcgcga tgttcgggta gtcgatggcg taggcgatgg
      361 cctggcggac cttgacgttt tcgaggccct tcttgttcag gttgaagatc agcagtggga
      421 tctggccagg cacgtggtac ggcttcttct tgctccacgt cgagaccggc ttgccggctt
      481 cccacatctt ccagatctgg gcggtgaact gctggctcgc gtcgatctcg ccgctctgca
      541 gcttcacgtc accgtcgttg ttgctcttga agatcgggtg gttgatcgtc gccggtgcgg
      601 gggtgccgta gaccgacttg ccccagtagt tgtcgtcgcg ggtcaggttg acctgcgtct
      661 ggtcgtactt gtccagcttg aacggcccgg atccgaccgg ttgcaggttg gtctcggacg
      721 ggatcttgtc cggggagatc ttgctgaaga ctgccttcgg cacgatcagc acggtcgaga
      781 ggtagttctt gaccatgttc gggttgtacg gcttggtctt gagcttgaac tcgaccgtct
      841 tcggatcgac ggcggtgatg gagtcgatgt actgccacac cgtggagaag ttcacgctcg
      901 cggtcttgcc gagctcgaag gtgaacacga cgtcatccgc ggtcaggtcc gaaccgtcct
      961 gccacttcgt gccgtcctgc aacggcacgg acatcgtcgt gtcgtctttc atgtcgaccg
     1021 tcttggccag accgggctgc agcgagccgt cgagcaggtt gaagcggagc aagctctcgt
     1081 agatgtactg cggctgaccg ttggcgaccg ggaagtccgg tgaggccgcg agtgggttga
     1141 agctcttcgg cgggccccac tggaacccgg cgacgaacag cgtctcgccg gaggcgccgg
     1201 ggcggccctt ctgctcgccg ctgccctgct ggctgccggt ctgggcggtg ccgcagccgg
     1261 cggcgctgac gccgatgacg gtcccgccga cggcgagacc ggtcaacgtg aggaaccgac
     1321 gacggctggg gttccaggac cgccagtccg cgccgctctc ggccatctcc gatggccggt
     1381 tgtcgtgcgt gtcgttcatg cttccctttc tcgagagcct ctcgacgagc cgaactcgcc
     1441 tttgcctgag ttgctgtgca ggggcggccc gccggtgtcc cgatgacgcg atggtccggg
     1501 aaccattgac actgccccga tcagttgtcg gtgcggcacc gcggagagag aggcgggaca
     1561 gggccgatcg gggccctcga tcagtacggg atcggctggt gggtcgatgg tcggcctgcc
     1621 cgcgagtcgc ggttcggcgc tggtgcccgt tttgatcagt tccgcttcga cgaaaaggga
     1681 cattgatcac ctgacggacg cctgaacgtt gccatggtcg tcgagcccct tcgctctcag
     1741 caagtcgctg cgacacagcc aagcacttaa ccgcttcaga gtcaacgcgt tccgataaat
     1801 ctgtggccag atcgttgtgt aagtgctact cgggctcctt tcgcgggccc gatgacttgc
     1861 cgtggcggtc gtggttaggt cggagagcga cttaaccgat tcacgaaacg gccgctcgag
     1921 tcggtacgga ccggggcacc ggtggaacgt gaacgagccc ggaacctgag aggttccggg
     1981 ctcatcgttc agaatgcctt cgaggcgtct gcctgtcgaa ggccccgact acgtcggtcg
     2041 gatcattcgt ggatcggtcg ggggatgctg cgattcgcga tccggttctc ggcgtcgatc
     2101 atccacgcca gttgttcgag tcgctcgaga atcgcatgca ggatgtccgc agtcgtcgga
     2161 tcctcggcgt ccacgtcgtc atggatccga cgggccgtgc cggccaccgc atagatagcg
     2221 gtgctgatca ggtcgacggt ctcgcccgtg tccacctcgt cggcggggaa gggcgccagg
     2281 gtggtcttgg ctgccaccgt ggcggcccgg ccgtccggcg tgacgtagat ggctcgcatc
     2341 cgctcggcga cggtgtcgga gaactcgcga gccgcgtcga cgagctcgtc tagctgcaga
     2401 tgaagatcgc ggaagttctt cccgaccacg ttccagtgtg cctgcttgcc ctgcaggtgc
     2461 aggtcgatca ggtcgacgtg caactgctgt aggtggtcgg cgagctgttg agacgcttgg
     2521 aaggggggat gcgctgcgtg ctctgtcgcc ggcttctcca aggtgtcact cattcggtcg
     2581 ctcctttgga ctgggtctgc ctctgtcgac tctagccaat cacgctttct ggaatgattc
     2641 cagtaaagca aggctctcct catcggcctc gcaccccgcg cgccgagtgc gcaccccggc
     2701 caaccggccc gcgccgacct cgcacctcgt acgtcgactt cgcaccccgg ccaaccggtc
     2761 caggcccact tcgcaccccg gacgtcgact tcgcaccctg cacgcaaggt gcgaagtgca
     2821 tctgcggggt gcgaagtggg acgggccggg ctgccgagag ctcacaacgg gacggcaagg
     2881 gcggagcggg cgcgcggtga agcgcgcatc gggcggacgg gtcgaccccc aatgggcccg
     2941 tgactcacgc ggtgggggtc gccacccagg tggggtcgat cgtggccagg atggcgccca
     3001 tggaccggcc gagctgcgga acgtcctcgg gacgtaccga atcgaagacc acgcgccgaa
     3061 ccagggcgac gtgaccaggg gcggtctcga cgaccttcgc gtacccctcg tcggtcagca
     3121 cggcgacggt ggcccgaccg tcggtcgggg aggtctcgcg ccggatccag ccacgcttct
     3181 gcagccgcgt gacggtgtgc gagagccgcg agagtgagcc gttcgagagc cgggcgagcg
     3241 tcttgagctg cagggtccgg tcgggtgtct cggagagcat ggcgagaacg aggtagtcga
     3301 acgtcgtcag gcccgagtcg cgttggagct gggagtccag caggctgggc aggagcagag
     3361 tgaccccgag cagacccaac cacgccgccc gctcgtcggc gtcgagccaa ctcggttcgg
     3421 tggattgctg gtccatggca gcagtctagc gttaaacttg actgttcaac tgaattttgt
     3481 gcgccgaatc aatcgatccg cttgccgtgg acggtcagcg aacggcggcc gcgctgctga
     3541 cgccatgctg tacggcctcc agcaaggcgg agttgaaggc cggaagatcg gccggcttgc
     3601 ggctggtgat caagttgccg tcgaccacga cctcctggtc gacccagttc ccgcccgcgt
     3661 tgcgaacgtc ggtccggatg ctcggccacg acgtgagttg cttgccgcgg acgacgtcgg
     3721 cttcgaccag cgtccaggcg gcgtggcaga tggacgcgac cggcttgccg gattcgacga
     3781 actccttgac gaatgcgacc gcggcgtcgt ccatccgcag tgcgtccggg ttggcgaccc
     3841 caccgggcag caccagggcg tcgtagtcgg ccacctgggc ctcgccgacg gccgcgtcga
     3901 ccgtgcgtgt gtcggccttg tcgaggtgct cgaacagttg cacgtcgccg gtcttcgggc
     3961 tgagcaggac gggctggtgg ccggcgtcgg ccaccgcgtc ccacggctcg accagttcgg
     4021 ccttctcgat cccttctgcc gcgaccagga acgcgatctt cttcgt
//
LOCUS       DATBFZ010000577         1917 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_5876985, whole genome shotgun sequence.
ACCESSION   DATBFZ010000577 DATBFZ010000000
VERSION     DATBFZ010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 1917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1917
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5876985"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1194)
                     /locus_tag="VH419_17615"
     CDS             complement(<1..1194)
                     /locus_tag="VH419_17615"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY7045493.1"
                     /translation="MSGVTAEPPVVVPPETGGEPGRWRQYALAAGFLAPAAVFLGIWV
                     LYPTVRTFGRSFFDNDGNFVWIDNYKEIFTTDVLRTAVKNNAIWVAVVPAFVTAIGLI
                     FAVLTERIRWSVAFKTVVFMPMAISAFAAGITWRIVYTQDPNLGALNAGIKVVHDSFG
                     AGGAIPDARPSNDELTGSTSQGFTTKSTLEPGDVARIGLTAIAPDELPARAVQAVQPE
                     PLQGGINGVVWRDFKPGGGVPGKVEQGELGIPGVTMKLLNADGDWVGNATSTDNGTFQ
                     FDDVSPGTYRVQVASATFAQPFGGVSWLGSKLITPAIMIAYIWIWAGFAMVVIAAGLA
                     AIPRDTLEAARTDGATEWQVFRRITVPLLAPVLSVVFITMIINVLKVFDIVVAVAPGS
                     VQDDAN"
     gene            complement(1235..>1917)
                     /locus_tag="VH419_17620"
     CDS             complement(1235..>1917)
                     /locus_tag="VH419_17620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7045494.1"
                     /translation="NIYLRTAGAEKYDQLSKHEIKWTDPSVKTALDEMAKVFSDTKNI
                     AGGTSGALQTDFPTSVSNVLSENPKAAQVMEGDFVPGVVKTSLQPETGYNQFKFPAVN
                     GSPEVVVGGGNLFIIFRDSPAVEAFIKYLATPESAEVWVERGGFSSPNKNVSTDKYPD
                     ELLKATSEALTNAETFRFDMSDLAPAAFGATVGQGEWKILQDFLKNPSDVAGTQQALE
                     SSAAKAFK"
BASE COUNT          300 a          653 c          640 g          324 t
ORIGIN      
        1 gttcgcgtcg tcctgaaccg acccgggggc gaccgccacg acgatgtcga agactttcaa
       61 gacgttgatg atcatcgtga tgaagacgac cgagagcacc ggcgcgagca gcggcacggt
      121 gatgcgccgg aagacctgcc actcggtggc accgtcggtt cgcgcagcct cgagcgtgtc
      181 gcgcgggatc gcggccagcc cggccgcgat gacgaccatt gcgaatccgg cccagatcca
      241 gatgtaggcg atcatgatcg ccggcgtgat cagcttggag ccgagccacg agacgccgcc
      301 gaacggctgt gcgaacgtcg ccgacgccac ctggacgcgg taggtccccg gcgacacgtc
      361 gtcgaactgg aacgttccgt tgtcggtcga ggtcgcgttc ccgacccaat cgccgtcggc
      421 gttcagcagc ttcatcgtca cgcccgggat gccgagctca ccctgctcga ccttgcccgg
      481 caccccgcct ccgggcttga agtcccgcca gacgacaccg ttgatgccgc cctgcagcgg
      541 ctcgggctgc accgcctgca cggcgcgggc cggaagctcg tcgggggcga tcgcggtcag
      601 gccgatgcgg gcgacgtcgc ctggctcgag cgtgctcttc gtcgtgaagc cctgactcgt
      661 cgaccccgtc agctcgtcgt tcgacggacg cgcatcgggg atcgcgccgc ctgcgccgaa
      721 gctgtcgtgc acgaccttga tgcccgcgtt cagcgcaccg agattcgggt cctgggtgta
      781 cacgatgcgc caggtgatcc cggccgcgaa ggccgagatg gccatcggca tgaagacgac
      841 cgtcttgaag gcgaccgacc agcggatgcg ctcggtgagc accgcgaaga tcaggccgat
      901 cgccgtcacg aacgcgggga cgacggcgac ccagatcgcg ttgttcttca ccgccgtgcg
      961 gaggacgtcg gtggtgaaga tctccttgta gttgtcgatc cagacgaagt tgccgtcgtt
     1021 gtcgaagaag ctccgcccga aggtgcgcac ggtcgggtag agcacccaga tgccgaggaa
     1081 gaccgcggcg ggcgcgagga acccggccgc cagcgcgtac tggcgccagc ggccgggctc
     1141 tccgcctgtc tcgggcggga cgacgaccgg aggctcggcg gtgacgccgc tcacgccggt
     1201 gtcacacctc ctgtcgcggc ccgcttcggc atcgctactt gaacgccttc gccgccgacg
     1261 actcgagcgc ctgctgggtg cccgccacgt cgctcgggtt cttcaggaag tcctgcagga
     1321 tcttccactc gccctgtccc accgtcgcgc cgaatgccgc gggggcgagg tcggacatgt
     1381 cgaaccggaa cgtctccgcg ttcgtcagcg cctcagacgt cgccttcagc agctcgtccg
     1441 ggtacttgtc ggtcgagacg ttcttgttcg gcgacgagaa cccgccacgc tcgacccaga
     1501 cctcggccga ctccggcgtc gcgaggtact tgatgaaggc ctcgaccgcg ggtgagtcgc
     1561 ggaagatgat gaacaggttg ccgccgccga cgacgacctc gggcgacccg ttcaccgccg
     1621 ggaacttgaa ctggttgtac ccggtctcag gctggaggct cgtcttcacg acgcccggga
     1681 cgaagtcgcc ctccatgacc tgtgcggcct tcgggttctc cgacagcacg ttgctgacgg
     1741 aggtcgggaa gtccgtctgc agcgcgcccg acgtcccgcc cgcgatgttc ttcgtgtccg
     1801 agaagacctt cgccatctcg tcgagcgcgg tcttgaccga cggatcggtc cacttgatct
     1861 cgtgcttcga gagctggtcg tacttctcgg cgccggcggt ccggagatag atgttct
//
LOCUS       DATBFZ010000578         9647 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_5878857, whole genome shotgun sequence.
ACCESSION   DATBFZ010000578 DATBFZ010000000
VERSION     DATBFZ010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 9647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9647
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5878857"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /locus_tag="VH419_17625"
     CDS             complement(<1..521)
                     /locus_tag="VH419_17625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020579008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HEY7045495.1"
                     /translation="MSRPRVSRRLCSITASASMVLAGLTVGVSARSAGDADEDVAATR
                     ALGLLAAARPVRVPGNFEGVGFDVCSAPDQATMDALRTESPYWGVGVYIGGANRTCDQ
                     PELTAAWIHTQHARGWHIFPIFIGLQAPVLHRGTSREVWCIDGSRHRKPMRANNEMAR
                     EQGRLAAIRAVGRA"
     gene            623..997
                     /locus_tag="VH419_17630"
     CDS             623..997
                     /locus_tag="VH419_17630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008360550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterol carrier family protein"
                     /protein_id="HEY7045496.1"
                     /translation="MPGRLRPLPATELAFLLARLDEAERADLEPSTDDVRAAVKHYLA
                     VLTMRAPGKSVEVRVPPYAVAQCIAGARHTRGTPPAVVETDAHTWLALARGRSTFADE
                     QRAGRLRASGERSDLSPVLPLT"
     gene            1042..2376
                     /locus_tag="VH419_17635"
     CDS             1042..2376
                     /locus_tag="VH419_17635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020389032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEY7045497.1"
                     /translation="MTRDLQQAIKDVLPGVRAELEALVRIPSVSADPARHDDVRRSAR
                     ATAALFEAEGLDTRVVEVDGGLPAVIARKPAPPGKPTVLLYAHHDVQPEGDHANWSTV
                     PFEPTERDGRLYGRGAADDKAGIAAHLAAIRAFGGDLPVGVTVFAEGEEESGSASLGT
                     ILSEHRDSLSSDVIVIADSGNWDIGVPALTTSLRGIVQLVVEVRTLSHAVHSGMWGGV
                     APDALTTLIQLLATLHDAEGNVAVDGLVSGPAADLDYPDDRLRTESGLLDGVETLGSG
                     SVVERLWTRPAISVIALDATKVADASNTLLPVATAVVSLRVAPGDDARRALQKLQDHL
                     TAHVPFGAQLSFPRSETGEPFAIRAIGPAYDAARAAFKEAWDGTDPVDAGVGGSIPFI
                     AEFAEIFPEAAILVTGVEDPDTRAHGANEGLHLAEFERVCLAEALLLRNLAT"
     gene            2522..4072
                     /gene="purF"
                     /locus_tag="VH419_17640"
     CDS             2522..4072
                     /gene="purF"
                     /locus_tag="VH419_17640"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012918301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEY7045498.1"
                     /translation="MPRGDGLLTHDLDPQERGPQDACGVFGVWAPGEDVAKLTYFGLY
                     ALQHRGQESAGIAVGNGRQILVYKEMGLVSQVFDEPTLSSLRGHVAIGHSRYSTTGAS
                     VWHNAQPTFRSTATGSLALGHNGNLTNTGDLAHRVDELARGHGQLDLATRTSEASSDT
                     DLVTALLAAYCDLPLEQAAVEVFPTIHGAYCFVFMDEGTLYAARDPQGIRPLVLGRLE
                     RGWVVASETAALDIVGATFVREVEPGELIAIDEDGLRSRRFAEPDPKGCLFEFVYLAR
                     PDTIISGQRVQTVRTEIGRTLARESPVEADLVIPVPESGTPAAIGYAEESRIPYGQGF
                     VKNSYVGRTFIQPSNTIRQLGIRLKLNALRDVIEGKRLVVVDDSIVRGNTQRAQVRML
                     REAGAREVHVRIASPPVKWPCFYGIDFATRAELIANGMTTDEICRSIGADSLAYVSLE
                     GLVAASKVAEGNLCRACFDGIYPVELPEPRFLGKSLLEQPIDAPLRPDGLSATMAGGG
                     AGDALTRP"
     gene            4116..5195
                     /gene="purM"
                     /locus_tag="VH419_17645"
     CDS             4116..5195
                     /gene="purM"
                     /locus_tag="VH419_17645"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008360561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEY7045499.1"
                     /translation="MSDQATSSTSTRSTYAGSGVSIEAGDRAVELMKTWVEKARRPEV
                     VGSIGGFAGLFDASALTTYERPLLASSADGVGTKVAIAQALDKHDTIGFDLVGMLVDD
                     LVVCGAEPLFMTDYIACGKVIPERIASIVKGISEACIEAGCALLGGETAEHPGLLGPA
                     EYDLAGSTTGVVEAAEVLGPSRVVAGDLLLALGSSGLHSNGYSLVRHVLLDRAGWSLD
                     RYVEVLGRSLGEELLEPTRLYTQACLALARTTDTHAMAHVTGGGLANNLARVLPDAVS
                     AVVDRSSWAPQPIFGLVADVGDVPRADLEATLNMGVGMVTVTPAEEVDVALTLLRDAG
                     VDGWVCGEVLDVPGGAVTLTGAHPD"
     gene            5425..5667
                     /locus_tag="VH419_17650"
     CDS             5425..5667
                     /locus_tag="VH419_17650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3073 domain-containing protein"
                     /protein_id="HEY7045500.1"
                     /translation="MGRGRAKAKQTKVARELKYREFNTDFSALQRELHGDDQDGTPET
                     DDTSSDQDDDEYDDPYADLYDDEDGMQAAESQGQSR"
     gene            complement(5826..6962)
                     /locus_tag="VH419_17655"
     CDS             complement(5826..6962)
                     /locus_tag="VH419_17655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HEY7045501.1"
                     /translation="MTDTRGTTADSADGSDTASGDVPAYSLFDTSSHEQVVFCHDEAS
                     GLKAIIAIYSTALGPGLGGTRFYPYATEADAVHDVLALSRGMAYKAALAGLDLGGGKA
                     VIIGDPHVDKTEALLRAYGRFVQSLNGRYLTACDVGTYSEDMDIVARECDFVTGRTVE
                     HGGAGDSSVLTAYGVFQGMRASAEVAWGTPTLHGKRVGVAGVGKVGHHLVEHLLHDGA
                     EVVVTDVYEPAVAAVRAAHPEVEVVADNDELVASDLDVYAPCALGSAINDDSVQLLKA
                     RIICGAANNQLAHPGIEEVLESRGILYAPDYCVNSGGLVQVADELEGFSFERAKARAT
                     KIYDTTKRVFAVAAAEGIATAVAADRIAERRMADVGRLRGIFLR"
     gene            7093..7908
                     /locus_tag="VH419_17660"
     CDS             7093..7908
                     /locus_tag="VH419_17660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7045502.1"
                     /translation="MLPYPSYLRVYEPLHALSSTVQAQLSADLDASAEAVSTLVTEQQ
                     TALARAVASPGLLLETEGRGSYVLRRDGHTYFCPTDMPLRSWLSLTSLVQSVGGATAH
                     LWFSAESLAVADEAFLRWRQAHPEAMPHIRQTTWGIPRTWFVLVAEDEREMYDVAGQT
                     SVRYRARMSDARRRMDAANAILSRVIDDADLMEELGDVSGWLEAFDDAGWVELDYAGV
                     ARLLGTALASDTSAGDIHRALAALRRGDFAGAGEAYRVFEERWRAVNAFERAN"
     gene            8179..8388
                     /locus_tag="VH419_17665"
     CDS             8179..8388
                     /locus_tag="VH419_17665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003949541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BldC family transcriptional regulator"
                     /protein_id="HEY7045503.1"
                     /translation="MNARPTESEPLLTPAEVAAMFRVDPKTVTRWAKAGKLSSIRTLG
                     GHRRYRESEVRELLAGVPGQRQADS"
     gene            8864..>9647
                     /locus_tag="VH419_17670"
     CDS             8864..>9647
                     /locus_tag="VH419_17670"
                     /inference="COORDINATES: protein motif:HMM:NF019145.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7045504.1"
                     /translation="MSAAVAVGLSLSAIPGSSGTTTTVSSRPSSETFGKAADSSTESK
                     ATYYDVRAGATPTQSAVLQRKAAAAGARQPTSRLRASLGSEAVVDMDGLTGTPRQLAR
                     LDGFLTRRSALPASAVAMGYVRTHATALGLTQRDIAGLRLARNYVDIAGTHHLSYVQY
                     AGGVPLFGNGLQANVTRDGRLLSLGGSPVSGLHAASVGSVITDGRSAVSTARRDQGDA
                     AKVGPADDARAVLFQTPTGTRRAWETITMSAEHPALTVLDSAT"
BASE COUNT         1501 a         3332 c         3305 g         1509 t
ORIGIN      
        1 gcccgtccaa ccgcccggat cgccgcgagc ctgccttgtt ccctggccat ctcgttgttc
       61 gcccgcatcg gcttgcggtg ccggctgccg tcgatgcacc agacctcgcg cgatgtgccg
      121 cgatgcagga caggtgcctg caggccgatg aagatcggga agatgtgcca gcctcgcgcg
      181 tgctgcgtgt ggatccaagc cgcggtgagc tcgggctggt cgcaggtgcg gttggcgcct
      241 ccgatgtaca ccccgactcc ccagtacggc gactcggtgc gcagcgcgtc catcgtggcc
      301 tggtcaggag cggaacagac gtcgaacccg acaccttcga agttgcccgg gacccggacc
      361 ggacgagccg cggccagcag cccaagagcc cttgttgccg ccacgtcctc gtcggcatca
      421 ccggccgacc gggcggagac tccgaccgtc agaccggcga gcaccatcga ggcggacgct
      481 gtgatcgagc agagcctgcg ggacacgcgt ggtcgcgaca tgtgctcacc ccgacgtcct
      541 gaacctgcct ctcgtggacc tcgcgtactg gaggctaaca gcccgtccga gcacgacgac
      601 gtacctcgct atgctgcgcg tcgtgcccgg tcgcctgcgc ccgctcccgg caaccgagct
      661 ggctttcctg ctcgctcgac tcgacgaggc agagcgcgcg gacctggagc cgagcacgga
      721 cgacgtccgg gcggcggtca agcactactt ggccgtgctg acgatgcggg ctccgggcaa
      781 gagcgtcgag gtgcgcgtgc cgccctacgc cgtggcgcag tgcatcgcag gtgcccgcca
      841 cacacggggc acgccgccgg cggtggtcga gaccgacgcg cacacctggc tggcgctcgc
      901 ccgcgggcgg tcgacgttcg ccgacgagca gcgggcaggg cgcctacgcg cctctggcga
      961 acgctccgat ctcagcccag tgctgccgtt gacctgacgt gcggcggatt cggccggccg
     1021 gacgatcact agagtcacat catgacccga gacctgcaac aagcgatcaa ggacgtgctt
     1081 ccaggggtgc gcgccgagct cgaggcgctg gtcaggatcc cttccgtcag tgccgacccg
     1141 gcccgccatg acgacgtacg ccggtctgcc cgcgcaacgg cggcgctgtt cgaagcagag
     1201 ggtctcgaca cgcgcgtcgt ggaggtggac ggcggacttc ccgcggtcat cgcccgcaag
     1261 cccgcgccgc cgggcaagcc aaccgtgctg ctgtacgccc accacgacgt gcagcccgag
     1321 ggcgaccatg cgaactggag cacggtcccg ttcgagccga ccgagcgcga cggccgcctc
     1381 tacggtcgcg gcgctgcaga cgacaaggcg gggatcgccg cacacctcgc ggccatccgc
     1441 gcgttcggtg gcgacctgcc cgtcggtgtg acggtgttcg ccgagggcga ggaggagtcc
     1501 gggtcggcat cactcgggac gatcctgtcc gagcaccgcg acagcctctc cagcgacgtg
     1561 atcgtcatcg ccgactccgg caactgggac atcggtgtgc ctgcgctgac cacgtcgctg
     1621 cgggggatcg tgcagctggt cgtcgaggtg cgaacgctgt cgcatgccgt gcattccggt
     1681 atgtggggcg gcgtggctcc cgatgcgctc acgacgctga tccagctgct tgccacgctc
     1741 cacgacgctg agggcaacgt cgcggtcgac gggctggtct cgggcccggc cgcggacctc
     1801 gactaccccg acgaccggct gcgtaccgag tcagggctgc tcgacggtgt cgagacgttg
     1861 ggctccggct ctgtcgtcga acggctctgg accaggcccg cgatctccgt catcgcgctc
     1921 gacgccacca aggtggccga cgccagcaac acactgctcc cggtggcgac tgctgtggtc
     1981 agcctccggg tcgctcccgg cgacgacgcc cggcgcgcgt tgcagaagct ccaggatcac
     2041 ctgacggcgc acgtgccgtt cggcgcgcaa ctgtcgttcc cgcgttctga gacgggtgag
     2101 ccgttcgcca tccgcgccat cggaccggcg tacgacgccg cgcgggctgc gttcaaggag
     2161 gcctgggacg ggactgaccc ggtcgacgcg ggtgtcggcg gctcgatccc gttcatcgcg
     2221 gagttcgcgg agatcttccc ggaggcggcc atcctcgtca ccggcgtgga ggacccggac
     2281 acccgtgccc acggcgccaa tgagggcctg cacctcgccg agttcgagcg agtctgcctc
     2341 gccgaggcgc tgctcctccg caacctcgcc acctgacccc tcacgccgag gtggcaggtt
     2401 tcgttgcaga cacgccgctt ggccggcgct gcttgccacc tcggcagctc ccccgagcga
     2461 ctcgcggcgt acgccgtgag gcaccggcgt tgtgggcacc gcgtccgcat agactgctgg
     2521 cgtgcctcgc ggagatgggc tgctcaccca cgatctcgat ccacaggaac gcggacccca
     2581 ggatgcatgt ggcgtcttcg gcgtctgggc ccccggcgag gacgtcgcca agctcacgta
     2641 cttcggcctc tacgccctgc agcaccgcgg ccaggaatcc gccggcatcg cggtcggcaa
     2701 cggtcggcag atcctcgtct acaaggagat ggggctggtc tcccaggtct tcgacgagcc
     2761 cacgctgagc tccctgcgcg gccacgtggc gatcggccac tctcgctact ccacgaccgg
     2821 ggccagcgtc tggcacaacg cgcagccgac gttccgttcg acggccaccg ggtcgttggc
     2881 gctcggccac aacgggaacc tcaccaacac cggtgacctc gcccatcggg tcgacgagct
     2941 tgcccgcggc cacggccagc tcgacctcgc cacccggacg tctgaggcct ccagcgacac
     3001 cgacctggtc acggcgctgc tggcggcgta ctgcgatctg ccgctggagc aggcggcggt
     3061 ggaggtcttc ccgacgatcc acggagcgta ttgcttcgtc ttcatggacg aggggaccct
     3121 gtacgccgct cgcgacccac agggcatccg gcctctcgtc ctcggccgcc tcgagcgagg
     3181 ctgggtcgtg gcatcggaga ccgcagcgct cgacatcgtg ggggcgacgt tcgtccggga
     3241 ggtggagccg ggcgagctca tcgcgatcga cgaggacggc cttcgcagcc gccggttcgc
     3301 ggagcccgat cccaagggat gcctgttcga gttcgtctac ctcgcacgtc cggacacgat
     3361 catcagtggc cagcgggtgc agaccgtgcg gaccgagatc ggccggaccc tggcccggga
     3421 gtcccccgtc gaggcggacc tggtcattcc cgtccccgag tccggtacgc cggcggcgat
     3481 cggctacgcc gaggagagca ggatccctta cggccaggga ttcgtgaaga actcctacgt
     3541 cggccggacc ttcatccaac ccagcaacac catccgtcag ctcggcatcc ggctcaagct
     3601 caacgccttg cgcgacgtca tcgagggcaa gcggctcgtc gtcgtcgacg actcgatcgt
     3661 ccgcggcaac acccagcgtg cgcaggtgcg gatgctgcgg gaggcgggag cgcgcgaggt
     3721 ccacgtgcgg atcgccagcc cgccggtgaa gtggccgtgc ttctacggca tcgacttcgc
     3781 gaccagggcg gagctgatcg cgaacggcat gaccaccgac gagatctgcc ggtccatcgg
     3841 cgcagactcg ctggcctacg tctccctgga agggctggtt gccgcgagca aggttgccga
     3901 gggcaacctg tgccgcgcct gcttcgacgg catctacccg gtcgagctgc ctgagccgcg
     3961 cttcctgggc aagtccctgc tggagcagcc gatcgacgcg ccacttcgtc ccgacgggct
     4021 gtcggcgacg atggcgggtg gcggcgccgg cgacgccctg acccgcccat gagcgcttcg
     4081 accgtccact cgcgcaggta cgccgaggaa ccgttatgag cgaccaggcc acgagttcga
     4141 cgtcgacccg atcgacgtac gccgggtcgg gcgtctcgat cgaggccggc gaccgagccg
     4201 tcgagctgat gaagacgtgg gtcgagaagg cgcgtcggcc cgaggtcgtc ggaagcatcg
     4261 gtggcttcgc ggggctgttc gacgcgtccg ccctgacgac gtacgagcga ccgctgctcg
     4321 ccagcagcgc ggacggcgtc ggcaccaagg tggccatcgc gcaggcgctc gacaagcacg
     4381 acacgattgg cttcgacctc gtgggcatgc tcgtcgacga cctcgtcgtg tgcggtgccg
     4441 aaccactgtt catgaccgac tacatcgcgt gcggcaaggt gatccccgag cgcatcgcca
     4501 gcatcgtgaa aggcatctcg gaggcctgca tcgaggctgg gtgtgcgttg ctcggcgggg
     4561 agacggctga gcatcccggc ctgctcggac ctgccgagta cgacctggcc gggtcgacca
     4621 cgggagtcgt cgaggccgct gaagtcctgg gaccgtctcg ggtcgtggcg ggtgacctgc
     4681 tgctcgcact ggggtcgtcg ggcctgcact cgaacggtta ctcgttggtg cgacacgtcc
     4741 tgctcgaccg cgccggctgg agcctcgacc ggtacgtcga ggtcctcggc cggtcgctcg
     4801 gcgaggagct gctcgaaccg acgaggctgt acacgcaggc ctgccttgca ctggctcgca
     4861 cgaccgatac gcatgcgatg gcccacgtca ccggcggggg gctggcgaac aacctggccc
     4921 gggtccttcc ggacgcggtc agtgccgtcg tcgatcgctc cagctgggca ccacagccga
     4981 tcttcgggct ggtcgccgac gtcggcgacg tcccacgtgc tgacctcgaa gcgaccctca
     5041 acatgggcgt cggcatggtc acggtgacac cggccgagga ggtcgacgtc gcgctcacgc
     5101 tgctccgcga cgctggggtc gacgggtggg tgtgcgggga ggtcctcgat gttccgggcg
     5161 gcgccgtgac gctcaccggc gcgcacccgg actgacgcgg actgacttgg actgaccctt
     5221 cgtcggggta cgcgcgttgc ccggcgctcg ggttccatcg cctggatcgg ccctgggtga
     5281 acttcaggac aacatcgagt cggacggcag accgcccgtt cgtgcgaagg cgtcgggcca
     5341 ggtatcgttg ggcccacgaa aaactgatga gggcccgggc gtccacgggg cggacccggc
     5401 acgaggtgcg agggggtcga ccctatgggg cgcggccgtg cgaaggcgaa gcagaccaag
     5461 gttgctcggg agctgaaata tcgcgagttc aacacggact tctccgctct ccagcgtgaa
     5521 cttcacggag acgaccaaga cggcactccc gagaccgacg acacctcctc ggaccaggac
     5581 gacgacgagt acgacgaccc gtacgccgac ctgtacgacg acgaagacgg catgcaggca
     5641 gccgagtccc aaggccagtc gcgctaggcc actcgcgagg tcacgggcag ccggtaccgg
     5701 tcccagccca cctgccgaat gcggtttgcg atgttgacag cccggacgcc gtctttacat
     5761 cgcaggtctg cggtgcaggg taggggtctg cgatggcgac tgatcggtga gtcccgacct
     5821 gtcgtctagc gcaggaagat gccgcggagg cggccgacat cggccatccg gcgttcggcg
     5881 atccggtcgg ctgcgaccgc ggtcgcaata ccctccgcag ccgccaccgc gaagacccgt
     5941 ttcgtcgtgt cgtagatctt ggtggctcgt gcctttgccc gctcgaacga gaacccttcg
     6001 agctcgtcgg cgacctggac cagaccgccc gagttcacgc agtagtccgg cgcatacagg
     6061 attccgcgtg actccagcac ttcctcgatc cccggatgcg cgagctggtt gttcgccgcc
     6121 ccacagatga tgcgtgcctt cagcagctgg accgagtcgt cgttgatcgc cgagccgagc
     6181 gcgcacggcg cgtagacgtc caggtcggag gccaccagct cgtcgttgtc ggcgacgacc
     6241 tcgacttccg ggtgagccgc gcgtacggcg gcgacggcgg gctcgtacac gtcggtgacg
     6301 accacctcgg caccatcgtg gaggagatgc tcgacgaggt ggtgcccgac cttgccgacg
     6361 ccggcgacgc cgacacgctt gccatgcaga gtgggcgtgc cccaggcgac ctcggcggat
     6421 gcgcgcatgc cctggaagac gccgtacgcc gtgaggacgc tggagtcccc ggcgcctccg
     6481 tgctcgacgg tccggccggt gacgaagtcg cactcgcggg ccacgatgtc catgtcctcg
     6541 ctgtacgtcc ccacgtcgca cgcggtgagg taacggccgt tcagggactg gacgaaccgg
     6601 ccgtacgcgc gcagcagcgc ctcggtcttg tcgacgtgcg ggtcgccgat gatgaccgcc
     6661 ttgccgccgc ccaggtcgag tccggccaac gccgccttgt acgccatccc gcgcgacagg
     6721 gcgagcacgt cgtgcaccgc atcggcctca gtcgcgtagg ggtagaagcg agtgccaccc
     6781 agcccggggc cgagcgcggt gctgtagatg gcgatgatgg ccttgagccc gctggcctcg
     6841 tcgtggcaga agacgacctg ctcgtgcgac gaggtgtcga acagggagta cgccggtacg
     6901 tcgccggatg cggtgtcgga gccatcggcg ctgtccgccg tcgtaccacg ggtgtcggtc
     6961 acggtagtga ctcttccttc gtgctcggtg tccgctgggg tggtgcggac tgtctcacac
     7021 caacgatagg gccaccgcga cgtactcgtg tgttctcggt caccgagccc cctgcgggga
     7081 tagcctgaat gcgtgctgcc gtacccctcg tacttgcgcg tctacgagcc gctgcacgcg
     7141 ttgagctcga ccgttcaggc acagctgtcc gccgacctcg acgcgagtgc tgaggctgtg
     7201 tcgacgctgg tgaccgagca gcagacggcg ctggcgcggg cggtggcgtc gccgggactg
     7261 ctcctcgaga ccgaaggccg cggcagctac gtcttgcggc gtgacggtca cacgtacttc
     7321 tgccccaccg acatgcccct gcgttcctgg ttgtcgctga cctcgctcgt ccagtcggtc
     7381 ggaggggcga cagctcacct gtggttcagc gcggagtcgt tggcggtggc cgacgaggcg
     7441 ttcctgcgct ggcggcaggc gcatccggag gcgatgccgc acatccgtca gacgacctgg
     7501 ggcattccgc gtacgtggtt cgtgctggtg gcagaggacg aacgcgagat gtacgacgtc
     7561 gccgggcaga ccagtgtgcg ttaccgggca cggatgtccg acgcccgacg acgcatggac
     7621 gcggccaacg cgatcctgag ccgggtgatc gacgacgccg acctgatgga ggagctcggc
     7681 gatgtcagtg gatggctgga ggcgttcgac gacgccggtt gggtcgagct cgactacgca
     7741 ggcgtggcgc gactgctcgg cacagccttg gcatccgaca cctcggcagg cgacatccat
     7801 cgtgcgctcg cggcactacg ccgcggcgac ttcgccggcg caggcgaggc ctaccgcgtc
     7861 ttcgaggagc gttggcgggc agtgaacgcg ttcgaacgcg ccaattgacc gcggcgcgtt
     7921 tcctgcacag aggaaatcgc caatcgcaac cggtgaaatt gtgtgcgcgc ggatctcccc
     7981 acattttgct cgacatacct ttgggtgctt cggctacgct ttagcgaagg atgaccgctt
     8041 agttccaccg ccgcgcacgt tgccgtccgg gcggtggcgc gcgaaggagt agtgcctcat
     8101 tgcgggtagc ctgcagcact aggacggaca aacggtccga ctgacggcag gtcggacacg
     8161 aagaaacgag gaggcggcgt gaacgcacga ccgaccgagt ccgaacccct gctcactccg
     8221 gccgaggtgg cggcgatgtt ccgcgtcgac cccaagaccg tgacgcgctg ggcgaaggcc
     8281 ggcaagctca gttcgattcg cacgctcggc ggccaccgcc ggtaccgcga gtcggaggtt
     8341 cgtgagctgc tcgccggcgt accgggtcaa cgccaggccg acagctgagt cggctgaccg
     8401 acacccgagt gtcagcgcac ctgatccgta cgagcatgaa agggccagca cgacccagat
     8461 gacactgccg gcagtgaaca accccgtgag gttcactcgg cagctgcacc acagcatcgc
     8521 ctgacttgac gacgaaggac caacggcgct cgccggtttc ccctgcagac cggatcgtcg
     8581 gcacggacag cacaggcgaa cggtgacggc gccctccaga ggggcgccgt cgcgcgtgtt
     8641 caggcaagcc aactcacgga cggatcaccg tgagatcacg gtcctgtcac ggccagatcg
     8701 ctgttcgcaa cggtaatgac cgcttcgccc ccagcctctg acatgcaacg gttgccgatc
     8761 ggccattccc aggggatagt tggcgcgttc atccgtaccc tcaggagggt cattccccgt
     8821 gcagtcctca ccacgacgcc ggcgccgcgc gctggcggca agcatgtccg cagcagtcgc
     8881 cgtcgggctc tccctgtcgg cgattcccgg gagctccggt acgacgacca ccgtctcttc
     8941 acgaccgtcg tccgagacgt tcggcaaggc cgcggacagt tccaccgagt ccaaggcgac
     9001 gtactacgac gtgcgggccg gggccactcc gacccagtcg gcggtgctgc agcgcaaggc
     9061 ggccgcggca ggtgctcgac agccgacgtc acgcctgcgc gcgtcgctcg ggagtgaggc
     9121 ggtcgtcgac atggacggcc tcaccggcac gccccgccag ctcgcgcgac tcgacggctt
     9181 cttgactcga cgcagcgctc ttccggccag cgcggtggcg atgggctatg tgcgtacgca
     9241 cgcgaccgcg ctcgggttga cgcagcgcga catcgccggt ctcaggttgg cgcgcaacta
     9301 tgtcgacatc gccggcaccc accatctgtc ctacgtgcag tacgccggcg gcgtaccgct
     9361 gttcggcaac ggactgcagg cgaacgtcac gcgggatggt cggctcctca gcctcggcgg
     9421 atcaccggtc agcgggctgc atgctgcatc agtcgggtct gtcatcaccg acggacgatc
     9481 ggcggtgtcg acggctcgtc gtgaccaggg cgacgccgcg aaggtcggtc cagccgatga
     9541 cgcccgcgcc gtactgttcc agaccccgac cgggacccgt cgtgcctggg agacgatcac
     9601 gatgtcggcc gagcaccctg cactcaccgt gctcgactcg gcgaccg
//
LOCUS       DATBFZ010000579         3639 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_5879122, whole genome shotgun sequence.
ACCESSION   DATBFZ010000579 DATBFZ010000000
VERSION     DATBFZ010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 3639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3639
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5879122"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1430
                     /locus_tag="VH419_17675"
     CDS             <1..1430
                     /locus_tag="VH419_17675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020392928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HEY7045505.1"
                     /translation="HEFDEGYKELLRMQDGGCIDDGDGQNNQNSCPDHEFKGANFQYL
                     SANVKYAKGQPQAGDTLFPAYRIERVDGIKVGFIGMTLKDTPTIVTRSGIEGLKFTDE
                     VVTANRLVPKLQKQGVKAIVVLLHQGVVPSDPSNYDGCSGVSGPALDIAQRLDPEIDA
                     IISGHTHQAYNCTVPDPRGHARLLTSASSFGRMVTDVHLMLNPRTHNVVRPAAWANNV
                     IVTNDNDGPDSDTLPDNDVTPVPALTRLITLYKTLVAPIANAVVGHIDPGTQVTRTMD
                     SDGESPAGDLIADSQLTDPSVIPPGGTAPQIAFMNPGGIRADLLENAAGDVTYEAAFT
                     MQPFNNYDVSMDLTGQQILDLLEQQWDGLNAGANNKILQVSGISYTYDKSNAADNSAR
                     AVDASTVMVDTDADGTPDTLLNPSSTYRIVCNSFLSDGGDNFGVFASGTNKYFGGLDI
                     DALTRYLGAHDPYNPVNKNLINSVP"
     gene            complement(1526..2899)
                     /locus_tag="VH419_17680"
     CDS             complement(1526..2899)
                     /locus_tag="VH419_17680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020387757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEY7045506.1"
                     /translation="MTDLTDLLQKAAAEAAAYRSSLDDRPVGPPIDLDAFVAGFDTEL
                     RAEPTAPDVVLDELVKVVDVGLVASAGPRYFGFVIGGSLPGAAAADILTTGWDQCAFN
                     AVLSPAAVTVEKVAGHWAKELLGIPTTASVGFVTGAQEANTVALAAARHHVLAQHGWD
                     VEQDGLVGAPRITVVASEERHATIDRSLRLLGLGNKAVVAVPADGNGAIETGRLARAL
                     ADVEGPTIVCLQAGNVSTGASDDLETAIAVAHDHDAWAHVDGAFGLWANAAPQRRHLV
                     RGVEAADSWGCDAHKTLNVPYDSGLVFVSRPDVHAAAASYTAAYLVGSGLGAAGGGAD
                     FALQSSRRAHGFTVWAALRELGRDGVGDLVERCCRLASRFADQLRDAGAQIGNDVVFN
                     QVLAAYGDDETTDAVISAVQRDGTCWMGGTTWKGRRYMRISVSNHTTTEADVDRSVDA
                     ILRCWKR"
     gene            complement(2948..>3639)
                     /locus_tag="VH419_17685"
     CDS             complement(2948..>3639)
                     /locus_tag="VH419_17685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020387763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY7045507.1"
                     /translation="RKYLLSSLDDSLTRMRLDYVDIFYSHRFDPDTPLEETMGALDSA
                     VRQGKALYAGISSYSAERTREAAAILRDLGTPLLIHQPSYSMLNRWIEPDLLDTLEEV
                     GAGCIGFTALAQGLLTSKYLDGIPAGSRAASEGTLDTDMFTDETMARVRGLNRVAEGR
                     GQSLAQLALAWALRDERMTSLVVGASSVRQLEENIKALEQLVFDDDELSRIDEHAVDS
                     GINLWASSSEA"
BASE COUNT          631 a         1274 c         1183 g          551 t
ORIGIN      
        1 accacgagtt cgacgagggc tacaaggaac tgctccgtat gcaggacggc ggctgcatcg
       61 acgacggtga cggacagaac aaccagaaca gctgccccga ccacgagttc aagggtgcga
      121 acttccagta cctctcagcg aacgtcaagt acgccaaggg ccagccgcag gccggcgaca
      181 cgctcttccc ggcgtacagg atcgagcggg tcgacggcat caaggtcggc ttcatcggta
      241 tgacgctgaa ggacaccccg accatcgtca ccaggtccgg tatcgaggga ctcaagttca
      301 ccgacgaggt cgtcaccgcg aaccggctcg tcccgaagct gcagaagcag ggcgtcaagg
      361 cgatcgtcgt gctgctccac caaggcgtgg tgccgagtga cccgtcgaac tacgacggct
      421 gctcgggagt ttcggggccg gcgctggaca tcgcacagcg cctcgacccg gagatcgacg
      481 cgatcatctc cggtcacacc caccaggcct acaactgcac cgtcccggac ccacgcgggc
      541 atgcgcggct gctcaccagc gcgtcttcgt tcggtcggat ggtgaccgac gtccacctga
      601 tgctcaaccc caggacgcac aacgtcgtgc gcccggcggc atgggccaac aacgtcatcg
      661 tgaccaacga caacgacgga cccgactcgg acacgttgcc cgacaacgac gtcacgccgg
      721 tgcccgcgct caccaggctc atcacgctct acaagaccct ggtcgcgccg atcgccaacg
      781 cggttgttgg acacatcgat ccgggcaccc aggtgacccg cacgatggac agcgatggtg
      841 agtcgccggc cggcgacttg atcgcggatt cccagctgac ggatccgtcg gtcatcccgc
      901 cgggtggcac cgcgccgcag atcgcgttca tgaaccccgg tggcatccgc gccgacctgc
      961 tggagaacgc tgccggtgac gtcacctacg aggcggcctt caccatgcag cccttcaaca
     1021 actacgacgt gtcgatggac ctcaccggcc agcagatcct cgacctgctc gagcagcagt
     1081 gggacggtct gaacgccggc gcgaacaaca agatcctgca ggtcagcggg atctcctaca
     1141 cctatgacaa gtccaacgct gccgacaact ccgcccgggc ggtggacgcg tcgacggtga
     1201 tggtcgacac cgacgccgac ggtacgccgg acacgctgtt gaacccgagc agcacgtacc
     1261 gcatcgtgtg caactcgttc ctgtccgacg gtggcgacaa cttcggtgtg ttcgcgtcgg
     1321 gtacgaacaa gtacttcggc ggactcgaca tcgacgccct gacgcggtac ctcggcgcac
     1381 acgacccgta caacccggtg aacaagaacc tcatcaactc agtgccctaa gcacgcgcgg
     1441 ccttccgcgg attgcgggaa aagtcaggct cggaccagcg tctcggcctc cactttcccg
     1501 caattcgcgg gaagccctgc ggtggtcagc gtttccagca gcgcaggatc gcgtcgacgg
     1561 accggtcgac gtcggcttcg gtcgtggtgt ggttggacac cgagatccgc atgtaacggc
     1621 ggcccttcca cgtcgtaccc cccatccagc acgtgccgtc gcgctggacc gcggagatga
     1681 ccgcgtcggt cgtctcgtcg tcgccgtacg ccgccagcac ctggttgaac accacgtcgt
     1741 tcccgatctg cgcaccggcg tcgcgcagct ggtcggcgaa cctgctcgcc agccggcaac
     1801 agcgctcgac aaggtcgcct accccgtcac ggccgagctc ccgcaacgcc gcccacaccg
     1861 tgaacccgtg cgcccggcgc gacgactgga gcgcgaagtc cgccccgccg ccggccgctc
     1921 cgaggccgga gccgacgagg tacgccgcgg tgtacgacgc agcagcggcg tggacgtccg
     1981 gacgggagac gaagacgagc ccggagtcgt acggcacgtt gagcgtcttg tgcgcatcgc
     2041 atccccacga gtccgcagct tcgaccccac gcacgagatg gcgtcgctgc ggtgctgcgt
     2101 tggcccagag accgaaggca ccgtcgacgt gcgcccaggc gtcgtggtcg tgcgccacgg
     2161 cgatggccgt ctccaggtcg tcgctcgcgc cggtgctcac gttgcccgcc tgcagacaga
     2221 cgatggtggg gccctcgacg tccgccaacg cccgcgcgag acggccggtc tcgatggcgc
     2281 cgtttccgtc agctggaacc gcaacgacgg ccttgttccc cagtcccagc agccggagcg
     2341 accggtcgat cgtcgcgtgc ctctcctcgc tggcgacgac ggtgatgcgg ggtgcgccga
     2401 cgagcccgtc ctgctcgacg tcccaaccgt gctgcgccag tacgtgatga cgcgcggcgg
     2461 cgagcgcgac ggtgttcgct tcctgcgcac ccgtgacgaa cccgaccgag gcggtcgtcg
     2521 ggataccgag cagctccttc gcccaatgac cggcgacctt ctcgaccgtg acagccgcgg
     2581 gcgacagcac ggcgttgaac gcgcactggt cccagccggt ggtcaggatg tccgccgcgg
     2641 cggcaccggg cagcgaacca ccgatcacga acccgaagta gcgtggcccg gcgctggcca
     2701 cgagtcccac gtccacgact ttgacgagct cgtcgagcac gacgtccgga gcggtcggct
     2761 cggcgcgcag ctcggtgtcg aaaccggcga cgaacgcatc gaggtcgatc ggcggcccga
     2821 cgggccggtc gtccagcgag gaacggtatg cggccgcctc ggcggcggcc ttctgcagca
     2881 ggtctgtcag gtcagtcacg tcgggaaggt acgccggctg cgcggcggcg acaactgccc
     2941 aaaggtctca cgcctcgctc gagctggccc acaggttgat gcccgagtcg acggcgtgct
     3001 cgtcgatgcg ggacagctcg tcgtcgtcga acacgagctg ctcgagcgcc ttgatgttct
     3061 cctccagctg gcggacgctg ctggcgccga cgacgaggga cgtcatccgt tcgtcgcgaa
     3121 gcgcccaggc gagcgccagc tgggccagtg actggccgcg tccttccgcg accctgttga
     3181 gcccacgcac gcgtgccatc gtctcgtcgg tgaacatgtc cgtgtccagg gtgccctccg
     3241 aggccgcccg agagccggcc ggaatcccgt ccaggtactt gctcgtgagg agtccttgcg
     3301 ccagcgcggt gaaaccgatg cagcccgcgc cgacctcttc cagcgtgtcg agcaggtcgg
     3361 gctcgatcca ccggttcaac atcgagtacg acggctggtg gatcaggagc ggagtcccga
     3421 ggtcgcgcag gatggcggcg gcttcgcggg ttcgctcggc ggagtacgag gagatgccgg
     3481 cgtacagcgc cttgccctgc cggaccgcgc tgtcgagcgc acccatcgtc tcctccagcg
     3541 gcgtgtccgg gtcgaaccgg tggctgtaga agatgtcgac gtagtcaagg cgcatccgtg
     3601 taagcgagtc gtcgaggctg gacaacaggt acttgcgtg
//
LOCUS       DATBFZ010000580         2390 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_5879776, whole genome shotgun sequence.
ACCESSION   DATBFZ010000580 DATBFZ010000000
VERSION     DATBFZ010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5879776"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1454)
                     /gene="uvrB"
                     /locus_tag="VH419_17690"
     CDS             complement(<1..1454)
                     /gene="uvrB"
                     /locus_tag="VH419_17690"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015582967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEY7045508.1"
                     /translation="MRPVTDLERTVAPFKVVSDYQPSGDQPTAIDDLQQRIEGGEKDV
                     VLLGATGTGKTATIAWLVERVQRPTLVMLPNKTLAAQFANELRELFPDNAVEYFVSYY
                     DYYQPEAYVPQTDTYIEKDSSINEEVERLRHSATNSLLTRRDVVVVSTVSCIYGLGTP
                     QEYVDRMVPLKVGTEVDRDSLLRRLVTIQYARNDLSFSRGTFRVRGDTLEIFPVYEEH
                     PIRVEFFGDEIERLMTLHPVTGEVLSEDSSLYVFPASHYVAGPERMERAIAGIEVELE
                     DRIVELERGAQLLEAQRLRMRTTYDIEMMRQIGTCSGIENYSRHIDGRAPGSAPNCLL
                     DYFPEDFLLVIDESHVTVPQIGGMYEGDMSRKRTLVEHGFRLPSAMDNRPLRWEEFQD
                     RIGQTVYLSATPGPYEMSRVDSTVEQLIRPTGLVDPQVIVKGTKGQIDDLIGEINART
                     ERGERVLVTTLTKKMAEDLTDYLLENGIRTRYLH"
     gene            complement(1488..2195)
                     /locus_tag="VH419_17695"
     CDS             complement(1488..2195)
                     /locus_tag="VH419_17695"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEY7045509.1"
                     /translation="MSPTSDDRTVVRRAWATLRPDGADVAVLTVAAVLYLLITADVVA
                     GGLMTRLDEVVRDATLPSGDPPAWTEVVGLLGNLGAMGGAALVVTIVVMQLTWRWWPG
                     VFAAAELAVTGLVVSTTKYLVDRPSPTGESPDGHLGFFPSGHTATAVIGMGTIVFLVS
                     LRADRPHAPRPGPRPALVAGVLAGALVASSSVFSGHHWLSDVLASLVVCAAILIVGCN
                     AVERHLEVGKVARKAAA"
     gene            complement(2192..>2390)
                     /locus_tag="VH419_17700"
     CDS             complement(2192..>2390)
                     /locus_tag="VH419_17700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013861185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HEY7045510.1"
                     /translation="SFADFHVAKHIGYALTGEVIGDQELAALLEPYRPHRYRVQHIVT
                     TRLPGPPRRGPRMASRAHLPR"
BASE COUNT          373 a          779 c          843 g          395 t
ORIGIN      
        1 ctgtggaggt agcgggtgcg gatgccgttc tccagcaaat agtcggtgag gtcctcggcc
       61 atcttcttcg tcagcgtggt gacgaggacg cgttcgccgc gctccgtgcg ggcgttgatc
      121 tccccgatca ggtcgtcgat ctggcccttc gtgcccttga cgatgacctg agggtcgacg
      181 agtccggtcg gccggatgag ctgctcgacg gtgctgtcca ctctggacat ctcgtacggc
      241 cccggggtcg ccgacaggta gacggtctgc ccgatccggt cctggaactc ctcccagcgc
      301 agcggccggt tgtccatcgc gctcgggagc cggaagccgt gctcgaccag cgtgcgcttg
      361 cgggacatgt cgccttcgta catgccaccg atctgcggga cggtgacgtg cgactcgtcg
      421 atgacgagca ggaagtcctc ggggaaatag tcgagcaggc agttcggcgc cgagcccggc
      481 gcccggccgt cgatgtgccg ggagtagttc tcgatgccgc tgcaggtgcc gatctggcgc
      541 atcatctcga tgtcgtacgt cgtccgcatc cgcagccgct gcgcctccag cagctgtgcg
      601 cctcgctcga gctcgacgat ccggtcctcg agctcgacct cgatcccggc gatggcgcgc
      661 tccatgcgct ccgggccggc gacgtagtgg gaggcgggga agacatacag cgacgagtcc
      721 tcactcagca cctcgccggt caccgggtgc agcgtcatga gccgctcgat ctcgtcgccg
      781 aagaactcca cccggatcgg gtgctcttcg tagacgggga agatctcgag cgtgtcgccg
      841 cggacccgga acgtgccgcg gctgaacgac aggtcattgc gggcgtactg gatggtgacg
      901 agcctgcgga gcaatgagtc gcggtcgacc tcggtgccga ccttcagcgg gaccatccgg
      961 tcgacgtact cctgcggggt gcccagaccg tagatgcagg agaccgtcga caccaccacg
     1021 acgtcgcggc gggtgagcag cgagttggtg gcggagtgcc tgagccgctc gacctcctcg
     1081 ttgatcgacg agtccttctc gatgtaggtg tccgtctgcg ggacgtaggc ctccggctgg
     1141 tagtagtcgt agtaggagac gaagtactcg acggcgttgt cggggaagag ctcgcgcagc
     1201 tcgttggcga actgggcagc gagtgtcttg ttggggagca tcaccagcgt cggccgctgg
     1261 acgcgctcga ccagccatgc gatcgtcgcc gtcttgccgg tgccggtcgc cccgaggagg
     1321 acgacgtcct tctcgccgcc ctcgatccgc tgctggaggt cgtcgatggc cgtgggttgg
     1381 tcgcctgacg gttggtagtc ggagacgacc ttgaacggcg cgacggtgcg ctcgagatcg
     1441 gtgacgggcc tcatgacatc gagcctacgg ggacgggccg acaagtccta cgcggcggcc
     1501 ttgcgtgcca ccttgccgac ctcgaggtgc cgctcgaccg cgttgcatcc cacgatcagg
     1561 atggcagcgc agaccaccag ggaggccagc acgtccgaga gccagtggtg tccgctgaac
     1621 acactcgatg aagcaaccag tgcccccgcg agcacaccgg cgaccaatgc tggtcgcggg
     1681 ccggggcgtg gggcgtgcgg ccggtcagcc cggagcgaga ccaggaacac gatcgttccc
     1741 atgccgatca ctgcggtggc cgtgtgtccc gacgggaaga atccgaggtg cccatcgggg
     1801 ctctcgcccg tcggcgacgg ccggtcgaca aggtacttgg tggtcgagac gaccagaccg
     1861 gtcacggcga gctccgccgc cgcgaagacg cccggccacc aacgccacgt cagctgcatc
     1921 acgacgatcg tgacgacgag cgcggccccg cccatggcgc ccaggttgcc gagcagtccg
     1981 accacttcgg tccacgcggg cgggtcgccg gacggcagcg tggcgtcacg tacgacctcg
     2041 tcgagccggg tcatcagacc accggcaacg acgtcggcgg tgatgagcag gtagagcacc
     2101 gcggcaacgg tgagaacagc cacatcggcc ccgtccggtc gcagggtcgc ccatgctcgg
     2161 cgtacgacgg tccggtcgtc ggatgtcgga ctcatcgcgg caggtgcgcc cgcgatgcca
     2221 tccgtggacc ccgtctgggc ggacccggca gtcgggtggt cacgatgtgc tggacgcggt
     2281 accggtgcgg acggtacggc tcgagcaggg ccgcgagctc ctggtcgccg atcacctcgc
     2341 cggtgagcgc gtatccgatg tgcttggcca cgtggaagtc ggcgaagctc
//
LOCUS       DATBFZ010000581        13804 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_6538985, whole genome shotgun sequence.
ACCESSION   DATBFZ010000581 DATBFZ010000000
VERSION     DATBFZ010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 13804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13804
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6538985"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..919)
                     /locus_tag="VH419_17705"
     CDS             complement(<1..919)
                     /locus_tag="VH419_17705"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY7045511.1"
                     /translation="MHDVLPFVITGLTTGAIYGLFGVGLVLTYKTSGIFNFAHGAIAT
                     AAAYLFYFLHVQHSWWWVPAAAVSVLVAGPLIGIIFQRMAARLSLQRTAYKIVATVGV
                     ILLVQALAVIKFGSNPLLVRPFLPGGRKEAFQVAGVRVSWDQLEAVLFALAAVSLLYA
                     VFRLTKLGVTTRAVVEDADLAAMRSVNPVAVRRLAWITGSTFAAASGVLFVNFVGVDS
                     IALTFLVVTALGAAAVGAFSSIPLTFLGALAIGVIADISKKYTIDVPLLNGLPDALPF
                     IILFIALLVTPRRKLVTPSVHTRPKEEYRT"
     gene            complement(1020..2102)
                     /locus_tag="VH419_17710"
     CDS             complement(1020..2102)
                     /locus_tag="VH419_17710"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY7045512.1"
                     /translation="MKLAYMINATGPAPKGQNVDVAKAWVAYTNAHGGVAGHPVDLEI
                     KDTKADAPTGQTFANELAKDPSVLGVMVLDPVGESTFIPTLVDQGLPVLGGVGSSQKV
                     WGVLPNVFAVATTPKTVANGFVLAAQDAGAKGIGVAACAEAEACSAGVPLFQAEANKL
                     GIKFTGVVKLDLNATSYTAECLQLMQNGTDFMLLAATAVAGIRLFNDCAQQGYKGNLG
                     ASVGAVTPDLYDGSPDIRLTGMIGAFPWWVDDAPVKQFRDVMQAGGVDAKKYGSPNGT
                     ATYTSLELFKKALESDSALPAEPTRADVLAAYGKLKDETLGGLLPKPLTFTPGQPGPD
                     VNCFWFYKYADGQFSGSFKPACDPDS"
     gene            2113..2355
                     /locus_tag="VH419_17715"
     CDS             2113..2355
                     /locus_tag="VH419_17715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7045513.1"
                     /translation="MGVEAAAVVAAAAAVVAAAPAVVAVAPAAAVVGLAATAVVALAA
                     AAVVWPAAPEPLPLLPQADTSSPQTLSTLATSHPAP"
     gene            2547..2981
                     /locus_tag="VH419_17720"
     CDS             2547..2981
                     /locus_tag="VH419_17720"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY7045514.1"
                     /translation="MSPEDRAALGDLVARYAQVADSRDPAAFAPLFTEDGTVTLVKDG
                     EPEVVRGPAQIVERLSRILRYDRTYHFLGQQTVELAGDGARGETYCLAHHLSTRDDGR
                     RDLMIAYVRYQDHYVRDGTTWRFGARTIVMDWTERRALDALR"
     gene            complement(3072..3962)
                     /locus_tag="VH419_17725"
     CDS             complement(3072..3962)
                     /locus_tag="VH419_17725"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEY7045515.1"
                     /translation="MLRNYADGPRDWRRLLDEAQLVDEVGVDRVVVVDHVVLGPHLDE
                     YPAGTFLTGPGGDWLEPMTTLAALAGVTRRVRLSTAIVIAGLRHPVVFAKTAATLDVL
                     AGGRLDLGVGVGWQRAEYEAVDLPFADRGRILDDALALWRRLWTPGPTRLGPDHREEV
                     WCEPSPVRDSGIPIWIGGGLSTAVLRRIVAHGAGWIPWARWANDVERGIDVIRAALIE
                     GGRSTDDFEVRARLPIISGDDQTIDLDATMERVERLMNAGVTDFSIQTQLSLDDLDRD
                     GERLSALVGAFRSKVGGAAF"
     gene            complement(4016..4963)
                     /locus_tag="VH419_17730"
     CDS             complement(4016..4963)
                     /locus_tag="VH419_17730"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY7045516.1"
                     /translation="MTTTEQRPTFPGVAAGTIVDTMVQVYVGPNGSNAPIPRESFRDA
                     ASLDAWPTMALVRYLFRGAGSARMDQAGELAAVVSMLDEWGVDIGIVPVSNDEAEAVL
                     EQLSRFGERFAAAVRVNPHTGMEAVRRLEALTRAFPVVKACAITPHFLWPPLPPNSKE
                     CYPIYAKCVELGLPVCINVGIPGPRVPGDCQNPMHLDEVCWFFPELTVVMKHGGEPWA
                     DVCVKLMLKWPNLFYTTSGFAPRYYPREIVEYANTRGGDRILYAGYFPMLGYDDVFLQ
                     LPSVPFRDHVWPRFLGENARRVFGLGRSANAERDAVLDG"
     gene            5028..5219
                     /locus_tag="VH419_17735"
     CDS             5028..5219
                     /locus_tag="VH419_17735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7045517.1"
                     /translation="MDDAGAVVRTLLEAAGISPPDDEVEELAESYARLRAMVDALYGP
                     AAATGAPAFLPHPAQVEPS"
     gene            5222..6610
                     /locus_tag="VH419_17740"
     CDS             5222..6610
                     /locus_tag="VH419_17740"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEY7045518.1"
                     /translation="MPSTITDAARALRRGDITSADLVQHAIDRAAAVDAALGTYIARF
                     DESALDAARAADKELAVGIDRGPLHGVPIGVKDIIAAREGVTTAGSRVLKGWGERRDA
                     TAVARLRDAGAVITGKTSTSEFAIGAPDDDGPFPVPHSPWDVSRWPGGSSSGSATGVA
                     AGLMLGALGSDTLGSIRIPSALCGISGLKTTFGRVPMSHVVPLSYTMDTVGPMARSAA
                     DCAIILQAIAGDDARDQYAADRAVPDYRAALVGELAGLRIGVEREHHTAVEGFNPLCA
                     ERFEAAIAVLRALGADVVDVRFEGYELMTAAALVTCGGDALAYHLGDLRTRYEDYGRS
                     CRKLLTRGVLYTAADYVQAQRVRAWGLSTLGDLFERCDAIVTPTTGVASPPSGADYWA
                     VMGPWTYTPAWSLLGVPAMSIPMGSIDEGLPSGLQIIGPHFSEARLLRIADAYQRSTD
                     WHLHEPPMVARS"
     gene            6714..8639
                     /locus_tag="VH419_17745"
     CDS             6714..8639
                     /locus_tag="VH419_17745"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF025114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY7045519.1"
                     /translation="MAPEVELLDEHIAGDEAIWAEAIQFANIPTLLMVLVHMTGDLKW
                     LDDPYRPSRTRGLDDNDTGGLPDEVQDEVRAAALETILAWSRGKPLAITEPSDELLVR
                     MLSVSVGYPIDAEYAPLITNELSLGKREAAPVQVPEGFRVLIIGAGISGLCAGVRLQA
                     AGIPFSIVERNDHVGGTWFENRYPGCGVDTPSYLYSFPFARARWSHYYALRNELYAYL
                     ESVADKFDLRRHITFGAEATSAEYDEDGQRWTVRARGRGGASLSYEANVVITAVGAFN
                     KPKLPKVEGVASFEGPVAHTARWPVDGIDVHDKRVGVVGTGASAMQLVPAIVDDAARV
                     IVFQRTPQWAAPFEKFKKPVPESVQHLLATVPLYYDWYRLRLAWTYNDAGYPALQKDP
                     TWDHPDRSTGPINDRHREIFSRYITAELGDRQDLLDKVLPTYPPYGKRMLMDNGWFRT
                     MTRDDVELVTQAVAEVRSQSVVTVSGAEYEVDVLVWATGFDVVHFLVPIDIRGRDGRD
                     LHAHWGDDARAYLGTTIPGFPNLFCLYGPNAQFGHGGSLITIMDRQMHYVMSVLTQMF
                     EAGAGAVEIKADVHDRYNAEVDARHENLVWTHPGMDNYYRNGRGRVVAINPFRVVEFW
                     GMTEHADLGEYHVEPAR"
     gene            complement(8676..9959)
                     /locus_tag="VH419_17750"
     CDS             complement(8676..9959)
                     /locus_tag="VH419_17750"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY7045520.1"
                     /translation="MAISELTEWDVLGDVHIIDCDSHLTEPPDLWSSRASASMRDRVP
                     VQRTVDGLTAWYLDGAVWASIGGNTIGTGHQKHLGTHMVQPFEKIDQAAWSAKERLAL
                     LDASGIWAQILYPNGIGFASNHIFAIPDERDRTVVLQIYNDFLVDIQEESGGRLLPQA
                     MLPYWDMDLTIKEMTRLLDKGIRGFTMSDKPEMIGLPELPEPYFDPMWDLFNESGAVA
                     NFHIGSGNRREDFEAIRASSGAMTGATESSGQKTLPLVAPPSWRTFGRQRRFAAIACQ
                     GYMSNVRIIVNLCLSDLFDRFPKLKVVSAESGIGWVPFVLEAMEFQLDEMVSDHDEVV
                     FQQRRPTEYFRDHLSVMFWFERSAPEKLITDVGVNNVLIETDIPHPTCLYPNPLRHFA
                     RVLGHLSPHVRQRVLQDNAAELYDVAIPVSDTRPA"
     gene            10080..11315
                     /locus_tag="VH419_17755"
     CDS             10080..11315
                     /locus_tag="VH419_17755"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY7045521.1"
                     /translation="MRVQKWGTSMSRIPPIISVDDHVVEPPHLWQTWLPAKWRERGPK
                     VVSAGYSFERVNGRRVETMTDTGPEVDWWVYEESLKLLPIQSHFAGRDRQTATDQPLR
                     FAELRPGYYDPKARLADMDLNHTERSLCFPNLLPRFAGQTFLEAKDKELALACVMAYN
                     DFIVEEWCGDSGGRLIPLGVVPLWDPALAAGEVRRNAARGCRAITFTELPANLGLPSL
                     HDRNRHWDPLFAACAETGTVICMHVGSGTTLLTTAPDAPTGMFAALINLNAMMSLTDW
                     LLSGVLMRFPALKIAYSESQIGWMPYMFERIDNVFVKARGWGGFDPALTDLPTTQIAG
                     RVYGCFFEDDFGLAVRDAVGIDQITFEVDYPHQDTTWPDSVAYLEKITTGLSDDEVHK
                     VVRGNAIRMLDLPAELPGS"
     gene            11331..13016
                     /locus_tag="VH419_17760"
     CDS             11331..13016
                     /locus_tag="VH419_17760"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY7045522.1"
                     /translation="MTHELVIRGGTVVDGTGLAAYRADVGVDGGLITEIGRIHDSGER
                     QIDAEGHVVTPGFIDVHTHMDAQVFWDPLGTPVCWHGVTTAVMGHCGFTLAPSAESDK
                     AFVIRNLERAEDISGEAMAAGIAWSWTTFAEYLDTVDRLPKGINYAANIGHSPLRTYV
                     MGERAFTEAANDDDLAGMCAQLGDALRAGAIGFTTSRSKNHQTSDDRPVASRLAEWSE
                     VDALIRHMGDSGGGIFQMVADWPPTAEARAAYEAGLVQLAAESGVPFALPAVGGNNDS
                     IMALLDRMAAAGGRAFGITHCRGIGSMSSFAARLPFDELAEWQPIRALPREEQTRALR
                     DPDVRARLIHAAHHGPYKDAVGAEARRPEFELMRVMDRPVPPNPTVAEVAAAHGVDPV
                     EAMIDLALENFETFFLQRFGRWDVENILKTMRHPRTVLGFSDAGAHVSQMCDASLPSH
                     LLAHWVRDRQDFTLEEGIRMLTLDQARWWGFHDRGVVRVGAKADLNVLDPATVTPDMP
                     TVVNDLPANQRRIKQTATGYLATIVNGRVTLEGGEPSGALPGQLLRGPLAVNR"
     gene            complement(13053..13700)
                     /locus_tag="VH419_17765"
     CDS             complement(13053..13700)
                     /locus_tag="VH419_17765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020576438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEY7045523.1"
                     /translation="MGQLLRVQNFAVSRDGFGAGEGQSLERPFGDADPAALMSWAGAT
                     ASWPNRTDPGGTRGLDDYFARDFKNNIGAEIMVRNKFGPQRGPWQNEEWKGWWGDVPP
                     FHTPVFVLTHHHRPSFTLDDTTFHFLDASPQEALRQAKAAADGKDVRLGGGVHTVHEF
                     LDADLVDAMHVAVAPVELGHGERLWKSHEELLDRFDVDVVPSPRSGVTHILLWRS"
BASE COUNT         2277 a         4668 c         4679 g         2180 t
ORIGIN      
        1 gtgtccgata ctcctccttc gggcgggtgt gcacagacgg agtcacgagc ttgcgccgcg
       61 gtgtgacgag cagggcgatg aacaggatga tgaacggcag cgcgtcgggc aggccgttga
      121 gcagtggcac gtcgatcgtg tacttcttcg agatgtccgc gatcaccccg atggccaacg
      181 cgccgaggaa ggtcaggggg atgctggaga acgcgccgac cgcggccgcg ccgagcgccg
      241 tgacgacgag gaaggtcagc gcgatgctgt cgacgccgac gaagttgacg aagagcacgc
      301 cggaggcggc cgcgaacgtc gagccggtga tccaggccag gcgacgcacg gcgacagggt
      361 tgaccgacct catggcggcg aggtcggcgt cctcgacgac ggcccgcgtg gtgacgccga
      421 gcttggtcaa gcggaacacc gcgtagagca ggctcactgc ggccagtgcg aacagcaccg
      481 cctccaactg gtcccagctg accctgacgc ccgcgacctg gaaggcctcc ttccggccgc
      541 ccgggagaaa tggccggacg agcagcgggt tcgagccgaa cttgatcacc gcgagcgcct
      601 gcaccaacag gatcacgccg acggtggcga cgatcttgta cgccgtgcgc tgcagcgaga
      661 gtcgagctgc catccgttgg aagatgatgc cgatgagcgg cccggcgacg agcacgctga
      721 ccgcggcagc aggcacccac caccacgagt gctgcacgtg caggaaatag aagagatacg
      781 cggcggcggt cgcgatggcg ccgtgcgcga agttgaagat gcccgacgtc ttgtacgtca
      841 ggacgagccc gacgccgaac agtccataga tcgcaccagt ggtcagtcca gtgatgacga
      901 acggcaggac gtcgtgcacg ctatgaggct cctagtgccg gcagtcgcga agacgacacc
      961 accagtgtgc cgcagtgccg tcctcgcccg ggctcacgca gagcgtgttc cgacctcgcc
     1021 tagctgtcgg ggtcacacgc cggcttgaac gacccggaga actgcccgtc ggcgtacttg
     1081 tagaaccaga agcagttgac gtcgggcccc ggttggccgg gcgtgaaggt cagcggcttg
     1141 ggcagcagcc ccccaagggt ctcgtccttg agcttgccgt aggcggcgag cacgtcagcc
     1201 cgcgtgggct cggccggcaa ggcgctgtcg ctctcgagcg ccttcttgaa cagctcgagc
     1261 gaggtgtacg tcgccgtgcc gttcgggctg ccgtacttct tcgcgtcgac acctcctgcc
     1321 tgcatgacat ccctgaactg cttgaccgga gcgtcgtcca cccaccacgg gaaggcgccg
     1381 atcatgccgg tcagacggat gtcgggagag ccgtcataca ggtctggcgt caccgctcca
     1441 actgatgcgc cgaggttgcc cttgtaccct tgctgcgcgc agtcgttgaa gagccggatg
     1501 ccggcgacgg cggtggcggc cagcagcatg aagtcggtgc cgttctgcat gagctggaga
     1561 cactcggcgg tgtagctggt ggcgttgagg tcaagcttca cgacgcccgt gaacttgatg
     1621 ccgagcttgt tggcctctgc ttggaacaac ggcacacccg cgctgcacgc ctcggcttcc
     1681 gcgcaggccg cgaccccgat gcccttggct cccgcgtcct gggccgccag cacgaacccg
     1741 ttggcgacgg tcttcggcgt ggtcgccacg gcgaacacgt tgggcagaac accccagacc
     1801 ttctggctcg agccgacgcc cccgaggacg gggagaccct gatcgaccag cgtcgggatg
     1861 aaggtcgact caccaacagg gtcgagcacc atgacgccga gcactgacgg atccttggcg
     1921 agctcgttcg cgaaggtctg cccggtcgga gcgtcagcct tggtgtcctt gatctcgagg
     1981 tcgaccgggt ggccggcgac gcccccgtga gcgttggtgt atgcgaccca cgctttggcc
     2041 acgtcgacgt tctgaccctt cggcgctgga ccggtcgcgt tgatcatgta ggcgagcttc
     2101 agaggtgacc cagtgggcgt cgaggcggct gctgtcgtcg ccgccgcggc cgcggtcgtc
     2161 gcagcggcgc ccgcggtcgt cgcagtggcg cccgcggctg cggttgtcgg actggcggcg
     2221 accgcggtgg tcgcgctggc ggcggccgcg gtcgtctggc cagcggctcc ggaaccgctc
     2281 ccgctgctcc cgcaggcgga caccagcagc ccgcagacgc tcagcacgct ggcgaccagc
     2341 catccggcac catgacgacc aaagacggat tgttgggtca tgagctcctc ccctcggact
     2401 cggccgacta tatgcataga ttctgttcag ccgcaaccgc gtcctgtccg cctcgatcct
     2461 ggaagcatcc cgcgacctcg ctccgtgcgc aggcgaacat ggcaaactcg ctgcatgctg
     2521 cgaatgattc gaaggcgtgc ggccagatga gcccggaaga ccgagctgcc ctcggcgacc
     2581 tcgtcgcgcg ttacgcgcag gtggccgaca gtcgggatcc agcggcgttc gcaccgctct
     2641 tcaccgaaga cggcaccgtc acgctggtca aggacggcga gccagaggtt gtccgcggac
     2701 cggcacagat cgtcgagcgc ctgtcgcgca tcctccggta tgaccgcacg taccactttc
     2761 tgggccagca gacggtcgag ctcgccggtg acggagcccg cggcgagacc tattgcctcg
     2821 cgcaccactt gagcacgagg gacgacggtc gacgggacct gatgatcgcc tacgtccgct
     2881 accaggatca ctacgtccgc gacggcacga cgtggcgctt cggggcgcgg accatcgtca
     2941 tggactggac ggagcgccgg gccctcgacg ccctgcgcta gtggaacgtt tcggagattc
     3001 ctgtgatgtc tcgccggccc tcggcgtccc cgatgactcg ccgatcccgc actcacgtcc
     3061 gaaacgctcc actagaaggc cgcgccgccg accttcgagc gaaacgcacc gacgagtgcc
     3121 gagagacgct cgccgtcgcg atcaaggtcg tcgagtgaga gttgggtctg gatcgagaag
     3181 tcggtcacgc ccgcgttcat gagtcgctcg acgcgttcca tggtcgcgtc gaggtcgatc
     3241 gtctggtcat cacccgagat gatgggcagc ctggcgcgaa cctcgaagtc gtctgttgat
     3301 cgtccaccct cgatgagggc ggctcgaatc acgtcgatcc cgcgctcgac gtcattggcc
     3361 cacctggccc acgggatcca acccgcaccg tgggccacga tccgccggag caccgcagtg
     3421 ctgagccctc cgccgatcca gatcgggata ccactgtccc ggaccggact cggctcgcac
     3481 cacacctcct cgcggtggtc cggaccgagt cgggtcgggc ctggggtcca aagtcgacgc
     3541 cagagcgcga gcgcgtcgtc gaggatgcgc ccgcggtcgg cgaacggcag atcgacagcc
     3601 tcgtactcgg cgcgttgcca gccgacgccg acaccgaggt cgaggcgacc gccggcgagt
     3661 acgtcgagcg tggccgcggt cttcgcgaac accacggggt ggcggaggcc ggcgatcacg
     3721 atggcagtcg agagccgcac gcgccgcgtc acgccggcga gcgcggccag tgtcgtcatc
     3781 ggctcgagcc agtcgccgcc agggccggtc agaaacgtcc cggccgggta ctcgtcgagg
     3841 tgcggaccga ggacgacatg gtcgacgacg accacgcgat cgacgcccac ctcgtcgacg
     3901 agctgcgcct cgtcgagcag ccggcgccag tcacgcgggc catccgcgta gttgcgcagc
     3961 atcatcgtga acgtcggagc gcggcgttcc ccttgcactg gcgagtcgtc ggtggctatc
     4021 cgtcgagcac ggcgtcgcgc tctgcgtttg cagagcggcc caagccgaag acgcgtcggg
     4081 cgttctcccc gaggaacctg ggccagacat ggtcgcggaa cgggaccgac ggcagttgca
     4141 ggaacacgtc gtcgtacccg agcatcggga agtagccggc gtacaggatg cggtcaccgc
     4201 cacgtgtgtt cgcgtactcg acgatctcac gcgggtagta acgaggggcg aaccccgacg
     4261 tcgtgtagaa gaggttcggc cacttcagca tgagcttcac gcagacgtct gcccaaggct
     4321 cgccaccgtg cttcatgacc acggtgagct cggggaagaa ccaacacacc tcgtcgaggt
     4381 gcatggggtt ctggcagtcg cccggcacac ggggtcccgg gatgcccacg ttgatgcaca
     4441 ccggaagccc gagctcgacg cacttcgcgt agatcgggta gcactccttg gagttcggcg
     4501 gcagcggcgg ccagaggaag tgcggtgtga tggcgcacgc cttcaccacc ggaaaggcgc
     4561 gggtcagggc ttccagccgg cgtacagctt ccatgccggt gtggggattg acccggacgg
     4621 ccgctgcgaa ccgttcgccg aagcggctca gctgctcgag cacggcctcg gcctcgtcgt
     4681 tggagaccgg gacgatgccg atgtcgacgc cccactcgtc gagcatggag accaccgcgg
     4741 cgagctcgcc cgcctggtcc atccgggcgc tcccggcacc acggaacagg tatcgcacca
     4801 gagccatcgt cggccacgcg tcgaggctgg cggcatcgcg gaaggactcg cgcgggatcg
     4861 gtgcgttcga tccgttcgga ccgacgtaca cctggaccat ggtgtcgacg atggtgccgg
     4921 cggcgacgcc ggggaacgtg ggccgctgct cagtcgtcgt catccgacgc ccctctccga
     4981 ctcgcgggta aacgataata tcatccgcag agtctggagg tgagcggatg gacgatgccg
     5041 gggcggtcgt caggaccctc ctggaggccg cggggatcag ccctccggac gacgaggtcg
     5101 aagagctcgc cgagagctac gcgcgattgc gggcgatggt cgatgcgctc tacggcccgg
     5161 cggccgcgac gggcgcgccc gccttccttc cccatcctgc gcaggtcgaa ccgtcgtgaa
     5221 gatgccatcg acgattacgg atgccgcccg cgccctgcgc cgaggcgaca tcaccagcgc
     5281 cgatctcgtc cagcacgcga tcgaccgcgc cgccgcggtc gatgccgcgc tcgggaccta
     5341 catcgcacgc ttcgacgagt cagccctcga cgcggcgcgt gccgcggaca aggaacttgc
     5401 ggtgggaatc gatcgcgggc cgctccacgg cgttccgatc ggcgtcaagg acatcatcgc
     5461 cgctcgtgag ggcgtcacga ccgcgggcag ccgcgtgctg aagggctggg gcgagcgacg
     5521 cgatgccacc gccgtcgccc ggcttcgtga cgcgggcgcg gtcatcaccg gcaagacctc
     5581 cacctcggag ttcgccatcg gcgcgcccga tgatgatggg ccgttcccgg tgccacacag
     5641 cccatgggac gtgagtcggt ggcccggtgg ctcgagctcg ggttcggcca ccggggtggc
     5701 cgccggcctg atgctcggcg cgctcggtag tgacaccctc ggcagcatcc ggatcccgtc
     5761 ggcgctctgc ggcatctccg gcctgaagac gaccttcggg cgggtgccga tgtcgcacgt
     5821 cgtgcccctc agctacacga tggacacggt gggtcccatg gccagatcgg cggctgactg
     5881 cgcaatcatc ctccaagcga tcgcgggtga cgacgcccgc gaccagtacg ccgccgaccg
     5941 tgccgtaccc gactaccggg ccgctctggt cggcgagctc gcggggttgc gcatcggggt
     6001 ggaacgggaa caccacaccg ccgtggaggg cttcaacccg ctgtgcgccg agcgattcga
     6061 ggcagcgatc gcggtgctac gtgcccttgg tgccgacgtc gtcgacgtgc gcttcgaggg
     6121 ctacgagctg atgacggccg ctgcgctcgt gacctgtggc ggcgacgcgc tcgcctacca
     6181 cctcggcgat ctgcgcactc ggtacgagga ctacggccgg tcgtgtcgca agctcctgac
     6241 ccgcggtgtc ctctacacgg cggccgatta cgtccaggcg cagcgagtcc gcgcgtgggg
     6301 tctgagcacg ctgggagacc tgttcgaacg gtgcgatgcg atcgtcacgc cgacgacagg
     6361 cgtggcgtcg ccaccgtccg gcgcggacta ctgggctgtg atgggaccgt ggacctacac
     6421 accagcgtgg agcctcctcg gggtgcccgc gatgtcgatc ccgatggggt cgatcgacga
     6481 ggggctgcca tccggcctgc agatcatcgg accccacttc agcgaggcgc gcctgctacg
     6541 tatcgcggac gcctatcagc ggtcgactga ttggcacctg cacgaaccac cgatggtcgc
     6601 gcgcagctag gagcccgcag ccggatatgc tgcgcatgat agtgtcggat tgcggtggac
     6661 agaccgacac cgcgtcgtgc ggtgtcgcgc cgagcgaagg ccgaggacgt gccgtggcgc
     6721 cagaagtcga gttgctggac gagcacatcg ctggagacga agccatctgg gccgaggcca
     6781 tccagttcgc gaacatcccg acgctgctca tggtgctcgt ccacatgacc ggcgacctca
     6841 aatggctcga cgacccgtac cgacccagcc gcacccgggg cctcgacgac aacgacaccg
     6901 gcggtctgcc cgacgaggtg caggacgagg tcagggctgc cgcgctcgag accatcctcg
     6961 cctggagtcg cggcaagccg ttggcgatca cggagccatc cgatgagctg ctggtccgaa
     7021 tgctcagcgt ctcggtgggc tatccgatcg atgccgagta cgcgccgctc atcacgaacg
     7081 aactgagcct ggggaagcgc gaggccgccc ccgtccaggt cccggagggg ttccgggtgc
     7141 tcatcatcgg cgctgggatc tcagggctgt gtgccggcgt ccgtctccag gcggcaggga
     7201 tccccttctc catcgtcgag cggaacgatc acgtcggcgg gacgtggttc gagaaccgct
     7261 atccggggtg cggcgtcgac acgcccagct acctctactc gttccctttc gcgcgtgcac
     7321 gctggtcgca ctactacgcg ctgcgcaacg agctctacgc gtatctcgag tcggtggcgg
     7381 acaagttcga tctgcggcgc cacatcacgt tcggcgccga ggcgacatcg gcggagtacg
     7441 acgaggacgg ccagcgatgg acggtgcgcg cccgtgggag gggcggtgca tcgttgtcct
     7501 acgaggcgaa cgtcgtcatc acagcagtgg gtgccttcaa caagcccaag ctgcccaagg
     7561 tcgagggagt ggcctcgttc gagggcccgg tggcccatac ggcgcgctgg ccggtcgacg
     7621 gcatcgacgt ccacgacaag cgggtgggcg tcgtcggcac cggcgcgagc gcgatgcagc
     7681 tcgtccccgc gatcgtcgac gacgcggcca gggtgatcgt cttccagcgc acgccgcaat
     7741 gggcagcgcc gttcgagaag ttcaagaagc ccgtgcccga gtcagtgcag cacctgctcg
     7801 cgacggtgcc cctgtactac gactggtacc ggctgcggct ggcgtggacc tacaacgacg
     7861 ccgggtaccc ggctctccag aaggacccga cgtgggacca tccggaccgg tccaccggtc
     7921 ccatcaacga tcgccatcgt gagatcttct cgcgctacat caccgccgag ctcggtgacc
     7981 gccaggacct gctcgacaag gtgctgccga cgtacccgcc gtacggcaag cggatgctga
     8041 tggacaacgg ctggttccgc acgatgacac gtgacgacgt cgagctggtc acgcaggccg
     8101 tcgccgaagt ccgatcgcag tcagtggtga cggtctcagg ggcggagtac gaggtcgacg
     8161 tgctcgtctg ggccacgggc ttcgacgtcg tgcacttcct cgtaccgatc gacatccgcg
     8221 gccgcgacgg ccgcgacctg cacgcccact ggggcgacga cgcgcgcgcc tacctcggta
     8281 cgacgatccc cggattcccg aacctcttct gcctctacgg ccccaacgcc caattcggtc
     8341 acggcggcag cctcatcacg atcatggacc gccagatgca ctacgtgatg agcgtgctga
     8401 cgcagatgtt cgaagccggt gccggcgccg tcgagatcaa ggccgacgtg cacgaccgct
     8461 acaacgccga ggtcgacgcg aggcacgaga acctcgtgtg gacccatccg gggatggaca
     8521 actactaccg gaacgggcgc ggccgggtcg tcgcgatcaa tccgttccgc gtcgtcgagt
     8581 tctggggcat gaccgagcac gcggatctcg gcgaatacca cgtcgagccg gcgcgctgag
     8641 accgccggcg cggcggtcgc tgcgcttgga gcgtgctacg ccggccgagt gtcgctgacc
     8701 ggaatcgcga cgtcgtagag ctccgcggcg ttgtcctgca gcactcgttg gcggacgtgt
     8761 ggcgagaggt gaccgagcac ccgagcgaag tgacgcagcg ggttcggata gaggcacgtg
     8821 gggtggggga tgtcggtctc gatgagcacg ttgttgacac cgacgtcggt gatgagcttc
     8881 tcgggcgcgc tccgttcgaa ccagaacatc accgacaggt gatcgcggaa gtactcggtg
     8941 gggcgccgct gctggaagac gacctcgtcg tggtcggaga ccatctcgtc gagctggaac
     9001 tccatcgcct cgaggacgaa cggcacccag ccgatgccgc tctcagccga gacgaccttg
     9061 agcttcggaa accggtcgaa caggtcgctg aggcagaggt tgacgatgat ccggacgttg
     9121 ctcatgtagc cctggcaggc gatcgccgcg aaacggcgtt gccgtccgaa ggttctccac
     9181 gagggtggag cgaccaacgg cagcgtcttc tggccggacg actccgtggc gcccgtcatc
     9241 gcgccgctcg aggcgcggat cgcctcgaag tcctcccgac ggttgcccga tccgatgtgg
     9301 aagttcgcga ccgcgccact ctcgttgaag agatcccaca tcggatcgaa gtagggctcg
     9361 ggaagctcgg ggaggccgat catctccggc ttgtcggaca tggtgaaccc gcggatcccc
     9421 ttgtcgagca gccgcgtcat ctccttgatg gtgaggtcca tgtcccagta cggcagcatc
     9481 gcctgtggga ggagccgtcc acccgattcc tcttggatgt cgacgaggaa gtcgttgtag
     9541 atctgcagca cgacggtgcg gtcgcgctcg tccgggatcg cgaagatgtg gttcgacgcg
     9601 aagccgatgc cgttcgggta gaggatctgg gcccagatcc ctgacgcgtc gagcagagcg
     9661 aggcgctcct tggccgacca cgccgcctgg tcgatcttct cgaagggctg caccatgtgc
     9721 gtcccaaggt gcttctggtg ccctgtgccg atcgtgttgc ctccgatgct ggcccacacc
     9781 gcgccgtcga ggtaccaggc tgtcaggccg tcgaccgtgc gctgcaccgg gacacggtcc
     9841 ctcatcgacg ccgatgcccg tgacgaccac aggtcgggcg gctccgtgag atgcgagtcg
     9901 cagtcgatga tgtgcacatc gccgagcaca tcccactcgg tcagttcgct gatggccacg
     9961 aggccctcct cacgggactt tcggatcgcg ccagcggttc gctcgcggcc ggcgccgcca
    10021 tccactcgac caaaccatac tatcatgcgc atggtaaact tcgtagagaa ccagggccga
    10081 tgcgcgtcca gaagtggggg acgtcgatga gtcgcattcc gccgatcatc tccgtcgacg
    10141 accacgtcgt cgaaccgccc catctctggc agacgtggct accggccaag tggcgcgagc
    10201 gaggaccgaa ggtcgtcagc gcgggctaca gcttcgagcg cgtgaacggc cggcgcgtcg
    10261 agacgatgac cgacactgga ccggaggtcg actggtgggt gtacgaggaa tccctcaagc
    10321 tgctgcccat ccagtcacac ttcgccggcc gcgaccgtca gacggccacc gaccaaccac
    10381 tgcggttcgc cgagcttcgt cccggctact acgacccgaa ggcgcggctg gccgacatgg
    10441 atctcaacca caccgagcgg tcgctgtgct tcccgaacct gcttccccgc ttcgccggcc
    10501 agaccttcct cgaggccaag gacaaggagc tcgcgctcgc ctgtgtcatg gcctacaacg
    10561 acttcatcgt cgaggaatgg tgtggcgaca gcgggggccg cttgatcccg ctcggggtcg
    10621 tgcccctgtg ggacccggcg ctggccgccg gcgaggtgcg acgcaacgcc gcccgcgggt
    10681 gccgggcgat cacgttcacg gagctgccgg cgaatctcgg gttgccgtcg ttgcacgacc
    10741 gcaaccgcca ctgggatccg ctgttcgccg cctgcgccga gactggaacc gtcatctgca
    10801 tgcacgtcgg ttctggcacc acgctgctga cgaccgcgcc ggatgcgccg acgggcatgt
    10861 tcgcggcact gatcaacctc aacgccatga tgtcgctgac cgactggctg ctgtccgggg
    10921 tgctgatgcg gtttcccgcg ctgaagatcg cctactcgga gagccagatc ggctggatgc
    10981 cctacatgtt cgagcgcatc gacaacgtgt tcgtgaaggc gcgtggctgg ggcggcttcg
    11041 atccggcgct cactgacctg ccgaccacgc agatcgcggg gcgggtgtac ggctgcttct
    11101 tcgaggacga cttcggtctc gccgtgcgcg acgccgtcgg catcgatcag atcaccttcg
    11161 aggtcgacta tccgcaccag gacaccacct ggccggactc ggtggcgtac ctggagaaga
    11221 tcaccaccgg gctgtcggac gacgaggtcc acaaggtggt gcgcggcaac gccatccgga
    11281 tgctcgacct tccggccgag ctgcccggtt cctgagcacg aagggctgag atgacacatg
    11341 aactcgtgat ccggggcggc accgtcgtcg atggcaccgg gctggcggcc taccgggccg
    11401 acgtcggtgt cgacggcggt ctgatcaccg agatcggccg catccatgac tccggcgagc
    11461 ggcagatcga cgccgagggt cacgtcgtca cgccgggttt catcgacgtg cacacccaca
    11521 tggacgccca ggtgttctgg gatccgctcg gcacgccagt gtgctggcac ggcgtgacca
    11581 ctgcggtcat gggccactgc ggcttcacgc tcgcgccatc ggcagagtcg gacaaggcgt
    11641 tcgtgatccg caacctggag cgggctgagg acatctcggg tgaggccatg gccgccggta
    11701 tcgcgtggtc gtggacgacc ttcgccgagt acctcgatac ggtcgatcgg ctaccgaagg
    11761 gcatcaacta cgcggccaac atcggtcact cgccgttgcg cacgtatgtg atgggcgagc
    11821 gcgcgttcac ggaagcagca aacgacgacg atctcgctgg catgtgcgct cagctcggcg
    11881 acgccttgcg cgccggcgcc atcggcttca ccacctcccg ttcgaagaac caccagacct
    11941 ccgacgaccg ccctgtggcc tcccggctgg ccgagtggtc ggaagtggac gcgctcattc
    12001 gacacatggg cgactcgggc ggcggcatct tccagatggt ggccgactgg cccccgaccg
    12061 ccgaggcgcg cgccgcctac gaagccggac tcgtccagct cgcggcagag agcggggttc
    12121 cgttcgcact gccggcggtg ggcgggaaca acgactcgat catggcgctg ctcgaccgga
    12181 tggcggccgc cggtggccgg gccttcggca tcacgcactg ccggggcatc ggctcgatga
    12241 gctcgttcgc ggcgcggctc ccgttcgacg agctggccga gtggcagccc atccgagcgc
    12301 tcccgcgaga ggagcagacg cgagccctcc gcgacccgga cgtgcgggcc cggctcatac
    12361 acgccgccca ccacggcccg tacaaggacg cggtcggtgc cgaagcacgg aggcccgagt
    12421 tcgagctgat gcgggtgatg gaccggccgg tgccgcccaa ccccaccgtc gccgaggtcg
    12481 ccgcagccca cggcgtggat ccggtggagg cgatgatcga cctggccctg gagaacttcg
    12541 agacgttctt cctccagcgg ttcggacgtt gggacgtcga gaacatcctc aagacgatgc
    12601 gccacccgcg cacggtcctc ggcttctccg atgccggcgc ccacgtcagc cagatgtgcg
    12661 acgcgtctct accaagccat ctgctcgccc actgggtgcg ggaccggcag gacttcacgc
    12721 tcgaggaggg cattcgcatg ctgaccctcg accaggcgcg gtggtggggc ttccacgacc
    12781 gaggcgtcgt ccgggtcggc gccaaagccg atctcaacgt gctcgacccc gccaccgtca
    12841 cgcccgacat gccgaccgtg gtcaacgatc tgccggcgaa ccagcggcgc atcaagcaga
    12901 cggccaccgg ctatctggcc acgatcgtga acggccgagt cactctcgag ggcggcgaac
    12961 cctccggcgc gctgcccggc cagctcctac gcgggcccct ggcggtcaac cgctagcggc
    13021 gtaggcccgt ggccccacgt cggcgccgcc ggtcagctcc gccagaggag gatgtgcgtg
    13081 acgccgctcc gcgggctggg cacgacgtcg acgtcgaagc gatcgaggag ctcctcgtgc
    13141 gacttccaca gccgttcgcc gtggccgagc tcgaccggcg ccacggcgac gtgcatcgcg
    13201 tcgacgagat cggcgtcgag gaactcgtgc acggtgtgca cgccgccgcc caggcgcacg
    13261 tccttcccgt ccgcggccgc cttcgcctgt cgcagcgcct cctgtggcga cgcgtcgagg
    13321 aagtggaagg tcgtgtcgtc gagcgtgaag gagggacgat gatggtgcgt cagcacgaac
    13381 accggcgtgt ggaagggagg cacgtcgccc caccagccct tccactcctc gttctgccac
    13441 ggcccacgct ggggtccgaa cttgttgcgg accatgatct cggcgccgat gttgttcttg
    13501 aagtcgcggg cgaagtagtc gtcgaggccg cgtgtgcctc ccggatcggt gcggttcggc
    13561 cagctcgcag tggcccccgc ccatgacatg agcgcggctg ggtccgcgtc gccgaacggc
    13621 cgctcgagac tctggccttc accggcgccg aagccgtcgc gcgacacagc gaagttctgg
    13681 accctcaaga gctgccccat cagagcgacg cctccagctg cttcagcagg ccgatcgtgc
    13741 cttcctcacg actggcgttc gcggccgcgc catccccgaa gtacgcgcct tgctcggtga
    13801 acac
//
LOCUS       DATBFZ010000582        23953 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_5881798, whole genome shotgun sequence.
ACCESSION   DATBFZ010000582 DATBFZ010000000
VERSION     DATBFZ010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 23953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23953
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5881798"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..696)
                     /locus_tag="VH419_17770"
     CDS             complement(<1..696)
                     /locus_tag="VH419_17770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7045524.1"
                     /translation="MTVTTGAGRIDVVAGAEYAQLGEATLSEVARRVLAVDDLHRATA
                     VLQPVSYDIGTVSTCALLRAVGRATVGGRTARWSAFVKVLQSPLAWELLHVIPDHLRD
                     RFVREFPWRLEIDALRSPVRSVLPDCLRMPELYAVHEPDDLHAALWMEDVDERTGPWS
                     LDTFARAARLLGELAGRRPVGSEAAFGDPLVRLQPGFGLRMYAESRVKHESAQVFASD
                     ATWADPVLVAALDD"
     gene            764..1252
                     /locus_tag="VH419_17775"
     CDS             764..1252
                     /locus_tag="VH419_17775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEY7045525.1"
                     /translation="MTVVKGSARPVPGIDAFEPKHMGFLLDRTMRRMRHDLYHLADAE
                     GIIGRYEPLRPSSFRLLSLIPAGGARVTDLAARASMTKQALGQFVDMLEPLRYVESNQ
                     DPADRRSRIITRTPLGDEAVADVNHLYELLDDQWRDIVGPRRWATFRAVMLELAIGWE
                     ES"
     gene            complement(1258..2478)
                     /gene="fahA"
                     /locus_tag="VH419_17780"
     CDS             complement(1258..2478)
                     /gene="fahA"
                     /locus_tag="VH419_17780"
                     /EC_number="3.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009080544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004334 - fumarylacetoacetase activity
                     [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetase"
                     /protein_id="HEY7045526.1"
                     /translation="MTWLSPSADSAYGIDTLPYGVFATEGSSTPRVGTRVGDQVVDLA
                     QVATVLEHPHAGVFASTSLNALMALDPSDWHEIRGWVRDLVTRDTHRGLVEQHLHDAS
                     AVTMLLPVDVADYVDFYASEVHASNVGKLFRPDAPDLPAAWRHLPIGYHGRGGTVVVS
                     GTDVVRPCGQRRPGADGIPPFGPSTRLDIECEVGFVVAGATVLGEPVPVAQARRHLFG
                     VVLLNDWSARDIQAWEYVPLGPFLGKSFATSISAWVVPFEALGEAEFDLLRQDPTPLP
                     YLHGESARDTFGLDLHLEVRLNDSLVSSPEYRDMYWSPAQMLAHLTVNGASLRTGDLF
                     ASGTVSGADRSTFGSLLELTWNGTEPITLADGSTRGFLEDGDVVTISGWAPGPDGGRV
                     TLGDVRGRVVAARC"
     gene            complement(2475..3305)
                     /locus_tag="VH419_17785"
     CDS             complement(2475..3305)
                     /locus_tag="VH419_17785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7045527.1"
                     /translation="MLSPVGSRGVSKELLFHDLLDDAAMFPPGNADATTALTRHLGYR
                     SGFMDAFVGPLLVHVDRWQDVVAAHAAIGSPPLEVVVIGGTEVHGAVPGLHVVGFELP
                     AHGLPLPQVAPGLGVACELRADETGLSLLGAIADAPGHVGKFRTGGTTPDAFPDEPTL
                     ARVVVAAALADAPMKYTAGLHHAVRFRDEESGFEHHGFLNLLAATHAALLGGTEAEVA
                     KLLGQRDGAVLAETVRSWSAQDVAGVRRAFVSFGCCGVEDPVRDLADLGLVDVEKESR
                     "
     gene            3344..4588
                     /locus_tag="VH419_17790"
     CDS             3344..4588
                     /locus_tag="VH419_17790"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009080540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006570 - tyrosine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase domain-containing
                     protein"
                     /protein_id="HEY7045528.1"
                     /translation="MAHYRSVGSVPPKRHTQHRPDGTTLVFEELMGEEGFSSDSSLLY
                     HRNLPSRIVAAEEWAMPALATRANHPLKPLHLKLHDLFDPEKIDDIDVVRGRRLVLGN
                     GDVRISYVVAGAASPLYRNGIGDECCYVERGSARVETIFGAFDVAQGDYVIIPRATSH
                     RWIPSGEGEPLRVYCIEANSHISPPKRYLSRFGQLLEHAPYCERDLRGPTGPLLAEDV
                     EAEADSDTEVYIKHRGNGPSGVVGTIFTYPFHPFDVVGWDGCLYPYVFNVSHFEPITG
                     RVHQPPPVHQVFEGYNFVVCNFVPRKVDYHPLSIPVPYYHSNVDSDEIMFYVDGDYEA
                     RKGSGIGRGSITVHPGGHAHGPQTGAYEKSIGVHYFDELAVMVDTFRPLELGEGGQAA
                     DDGRYAWSWARGGQAAEPPSHG"
     gene            4847..5428
                     /locus_tag="VH419_17795"
     CDS             4847..5428
                     /locus_tag="VH419_17795"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEY7045529.1"
                     /translation="MVSNRVLTVPNALSIVRLCGVPLFLWLVLGPQLDGWALLVLAVS
                     GITDWLDGKLARTLNQSSRLGQILDPVADRCYILAVVIGLALRDIIPWWLAVGLPLRD
                     VFLFCLVPFLRTRGYSALPVHFLGKAATACLLYAFPLLLLGDGDSTLARLADVFGWAF
                     AIWGTALYWWAGILYAYQVVQLMRTPRVAEEVR"
     gene            5425..6294
                     /locus_tag="VH419_17800"
     CDS             5425..6294
                     /locus_tag="VH419_17800"
                     /inference="COORDINATES: protein motif:HMM:NF017734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HEY7045530.1"
                     /translation="MSDEEPSTSTPPVSQQPRAYVGLLTEVMSNTLDEDYATVAAKRP
                     SGPVESPRGRRTRNNRIALTSVVVAFGLMLGISALKTDKDRPVTRAERDELVAQIHNR
                     QHEQAQLAAQLNAVQDDVTDLQNRVGTEASRNSQITDQLVALGVQAGTLAVEGPGMVI
                     TVDDAPSADEGSGGTILDTDLQALVNGLWAAGAEAVSIDGHRLTSLTAIRFAGSAITV
                     DNVSLAPPYVITAIGNPDTLPARLLETEGGQTWLGLEANFGIRFDRETKENVVVPGDP
                     HDHLLYAEPDTGG"
     gene            6302..6634
                     /locus_tag="VH419_17805"
     CDS             6302..6634
                     /locus_tag="VH419_17805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020100154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic family protein"
                     /protein_id="HEY7045531.1"
                     /translation="MIAVFGLVVGILAGLFLEPSVPASLQPYLPIAVVAALDAVFGGL
                     RAFLDGIFDDKVFVVSFVSNVLIAALIVYLGDQLGVGGQLSTGVIVVLGIRIFSNVAA
                     IRRHLFHA"
     gene            6640..7428
                     /locus_tag="VH419_17810"
     CDS             6640..7428
                     /locus_tag="VH419_17810"
                     /inference="COORDINATES: protein motif:HMM:NF017734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HEY7045532.1"
                     /translation="MSSDNADRDDRDPGSEETMDSGGASGRQRLADALRARPSRAQFV
                     VALLLCALGIAAATQIRLTHSDDDFAGQRREDLVDLLDSLSSATDRARTQLDDLEQTR
                     NELQSSSARRAAVLEEGQQRLAVLGILSGAVAATGPGVTITIDDPRGSVQAATLLDGI
                     EELRDAGAEAIEINDSVRVVATTSFIDGDGEIIVDGETLRPPYVIDAIGASHTLSEAV
                     VFPGGLSDDVERDGGTVTVKEADVVEVGSLHPIEQPQYAQPTNR"
     gene            7453..7839
                     /gene="gcvH"
                     /locus_tag="VH419_17815"
     CDS             7453..7839
                     /gene="gcvH"
                     /locus_tag="VH419_17815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020659841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEY7045533.1"
                     /translation="MAYPDDLKYTAEHEWVKAGEGSTVRVGITDYAQEALGDIVYVNL
                     PDVDTTVEAGSAMGELESTKSVSDLFAPVSGKVTARNEALDANPELCNSDPYGEGWIV
                     EIDADDASAVEGLMSADDYEATVTSN"
     gene            7938..8444
                     /locus_tag="VH419_17820"
     CDS             7938..8444
                     /locus_tag="VH419_17820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEY7045534.1"
                     /translation="MPFCTECGHQNPDGARFCSNCGHRLVATEAPAESTSTLSFGGPD
                     RSEPQSTGLSDADEAAVNALPRGSALLVVQRGSSAGSRFLLDSDEVTAGRHPDSEIFL
                     DDVTVSRRHAVFRRTSDGYLVADVGSLNGTYVNRDRIDEVLLVTGDEVQIGKYRLVYF
                     NSPQDTDT"
     gene            8477..9193
                     /locus_tag="VH419_17825"
     CDS             8477..9193
                     /locus_tag="VH419_17825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017936315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEY7045535.1"
                     /translation="MSIGEVLNRLRSDFPDVSISKIRFLESEGLIEPERTPSGYRKFS
                     YGDLERLRYILSEQRDHYYPLKVIKEHLDAMERGLEPPAVAGESPRAPHSVRSLGIVT
                     EDASDFGPAPASDLRMSRAELLANSGLTDQQLTELMEFGMLRPRPGTRYFDADALVVA
                     ETIAQLTAFGLEARHIRAFKAAADREVGLIDQIVSPLSRGRDDNAAARAADTAAELGA
                     LSVRLHAALVKTGLRRPHPR"
     gene            9326..9826
                     /locus_tag="VH419_17830"
     CDS             9326..9826
                     /locus_tag="VH419_17830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008359554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HEY7045536.1"
                     /translation="MREVDVIGVRVEMPSNQPLVLLRESSGERYLPIWVGAVEATAIA
                     FAQQGVVPPRPLTHDLMKDLLVATGHELHEVQITEVRDGIFYAILVLGDGVEVSARPS
                     DSIALALRTGSRIVCSDEVLEEAGMAVPDDQEEEVEKFREFLDQINPEDFGPYQGQRG
                     ETETGE"
     gene            10069..10626
                     /locus_tag="VH419_17835"
     CDS             10069..10626
                     /locus_tag="VH419_17835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017936317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEY7045537.1"
                     /translation="MAAAIDTASGQGLLFDDDVAPMPDDVGYRGPMACAAAGITYRQL
                     DYWARTGLVEPTVRTAGGSGTQRLYSFRDILLLKVIKRLLDAGVSLQQIRSAVHHLRE
                     RGTDDLTEVTLMSDGASVYECRSAEEVIDLVQGGQGVFGIAIGGVWREIEGSLSELPS
                     ERAARDDLEHPGDELAARRRARHAG"
     regulatory      10697..10805
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            10883..13780
                     /gene="gcvP"
                     /locus_tag="VH419_17840"
     CDS             10883..13780
                     /gene="gcvP"
                     /locus_tag="VH419_17840"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HEY7045538.1"
                     /translation="MTDRQPIPTLRELESAAPFSRRHIGPDAAEQRHMLDTLGFAGME
                     DLLYAALPDKIRTDKPLDLPPALSEVGARDALGAIAARNNVKVPMIGLGYSGTVTPAV
                     IRRNVLEDPSWYTAYTPYQPEISQGRLEALLNFQTMVADLSGLPIANASLLDEATAAA
                     EAMTLVRRANRSNASARFVVDEQALPQTLAVVRTRAEAMGITVDVRDVYAGLPSDDLA
                     GVLIQYPDAHGRVRDPRPVIEAAHAAGALAVVAADLLSLTLLEAPGELGADVVLGSSQ
                     RFGVPLFYGGPHAGFMAVRTGLERHLPGRLVGVSVDAEGRPAYRLALQTREQHIRRDK
                     ATSNICTAQVLLAVAASMYAVYHGPTGLTRIARRTHRYATVLAEALRQTGQEVEHAHF
                     FDTVSVRVDGEADAIRVRAGDLGLHVLADGADRVGVSLSELTTREHLALVLDAFDVRP
                     DSVDWDALDSSANDPIPEELRRRTPFLTHPVFNDHRSETDLLRYLKRLSDRDYALDRG
                     MIPLGSCTMKLNATAEMEPISLPGFADLHPFVPAEDALGYVELVESLERWLAEVTGYA
                     EVSIQPNAGSQGELAGLLAIRGYHRARGETGRDVCLIPSSAHGTNAASAVMAGMRVVV
                     VKATDEGAIDLDDLRARCETHTDELAAIMVTYPSTHGAYEDTITELCSIVHGHGGQVY
                     VDGANLNALLGLAKPGEFGGDVSHLNLHKTFCIPHGGGGPGVGPVAVAAHLAPFLPSH
                     PLHPERGRRDGIGPVSAAPYGSAGILPISWAYIAMMGQAGLIEATTVAVLSANYIARR
                     LAEHYPVLYKGHGDLVAHECILDLRQLTKASGVSVDDVAKRLIDYGFHAPTMSFPVPG
                     TFMVEPTESENLAELDRFIDAMIAIRAEIADVEAGRTDLSSSPLVHAPHTTAAVTGDW
                     ERSYSRAVGAFPAGHSADKYWPPVARIDQAYGDRHLVCSCPDPSAFAEDGS"
     gene            13777..15075
                     /locus_tag="VH419_17845"
     CDS             13777..15075
                     /locus_tag="VH419_17845"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEY7045539.1"
                     /translation="MTEEATGDRRSVLVADAHTKGRLPGIVAATSRDGVVDWRCVFGD
                     TGAAYRIASITKTFTAVAVMQLRDEGRIDLDEAIGRHVPETPYADQAIRRLLSHSSGM
                     TAEPAGPWWERVPGGSWSELVAANRRATHVFKPGERYHYSNLGYGLLGELVARLRGRS
                     WWDVVTERIVEPVGLTGTTFARPDDAAVGTSRNPLTDELVREPSEDEGAMAAAGQLWS
                     TPDDLLRWVDVLAAGHPGVLSADTAVEMRTVQSGDPDLQHRGAYGLGLRLRWSDQATL
                     IGHSGSLPGFLAGMFADPVSRVGAVVLTDATYGLDPEGVCASLIESAPPAQAQPPVAD
                     PASPATDLAGAWYWGNVPFELVPTASGAIVISGANRSRIEQVGVDVYRGLNGYWAGEL
                     LHVHRDADGRARHLDVVTFILTRTPYDPKAPIPGRTPEPL"
     gene            complement(15158..16540)
                     /locus_tag="VH419_17850"
     CDS             complement(15158..16540)
                     /locus_tag="VH419_17850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HEY7045540.1"
                     /translation="MATPQALVEHALDVSTADHCAVILTLSSTANLRWANNTLTTNGV
                     MTASHLTVVSVVDGRQGVSAGVLTQSASTPGQVSDLVARADATARAASPADDARPLVN
                     GDAASSWDDPAGTTSIDVFEDFAVALGDVLGRARDDDRLLFGYVEHDVTTTYLATSTG
                     LRLRHEQPTGHIGITGKPGDLTTSAWVGQAAERIDEIDLSALDAELVRRISWATRKVE
                     LPAGRYDTVLPPTAVADLMIYAYFVASGQDAYDGRTVYSDPDGGTKVGQRIAATGVRL
                     SSDPATPGLAAAPFLTARATSSAQSVFDNGLTLAKTDWIADGTLRHLGTSRHTADQTG
                     LPVAPLIDNLSLEIASGHGALDDVVAEVDRGLLVTCLWYIRSVDPQTLLLTGLTRDGV
                     YLVEGGEVVGAVNNFRFNESPIDLLNRFSRAGETVRSFSREWGDHFPRTATPPLRLPD
                     FNMSSVSQAS"
     gene            complement(16540..18054)
                     /locus_tag="VH419_17855"
     CDS             complement(16540..18054)
                     /locus_tag="VH419_17855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEY7045541.1"
                     /translation="MPTSHGIDLEFADLPLARLASTALQRAQDLKVEHADLRVERLRT
                     QHLQLRDGGLVDASEGDDVGFAVRVVHDGTWGFAAGVTLSVEDVVRVTEQAVRVAMVA
                     KAITHERIELAPEAVYADATWVSSYDVDPFEVSTPDKVAVLAEWSQRLLGAPSVDHVR
                     ATVQQVRENKFYADSHGTTTTQQRVRINPQFEVFGADHDRGLLDGMRTIAPPTGRGWE
                     YLIDGAYDWDTELAELPDLLSAKLAAPSVDAGRFDLVIHPSNLWLTIHESIGHATELD
                     RALGYEANYAGTSFATIDKLGSLEYGSRHLNITGDRTVEHGLASIGFDDDGVGTQAWD
                     IVKDGILVGYQTDRRMAARMGWERSTGCAFADSPGHIPIQRMANVSLAHSPEDISTED
                     LISQVERGIYIVGDKSWSIDMQRFNFQFTGQRFFAIENGTLSHPLRDVAYQATTTDFW
                     GSMEAVGGADTWVLGGAFNCGKAQPGQVAPVSHGCPSALFRDVNVLNTTHEAGR"
     gene            complement(18073..18312)
                     /locus_tag="VH419_17860"
     CDS             complement(18073..18312)
                     /locus_tag="VH419_17860"
                     /inference="COORDINATES: protein motif:HMM:NF039316.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5999 family protein"
                     /protein_id="HEY7045542.1"
                     /translation="MRSVISGSTCTHEPLCPSAEAADREAAHTVAAHPEQGWSLLCNG
                     VLLFEDTGELLPNGDVIAPHRPTDTHPEVFGHLVS"
     gene            complement(18366..21086)
                     /locus_tag="VH419_17865"
     CDS             complement(18366..21086)
                     /locus_tag="VH419_17865"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HEY7045543.1"
                     /translation="MRIHRLELTAFGPFPGTEVVDFDRLNDAGLFLLTGPTGSGKTSV
                     LDAVCFALYGAVPGNRNAAKSFKSHHAAPATVPSVLLEVTVRGRRLRLRRQPAWTRPS
                     RRARSGVVEETAKASVEEYVEGGWHVHSTRVDEVGHLVSSILGMNKDQFCQVVMLPQG
                     EFQTFLTAGAKDRHDVLETLFRTHRFNAVERWLTDSRRALDAECRAVEHTMDRLLARV
                     EEAFAGAPTAAKDVGHAADTHTHDQADEHDYAAMVERLQARREVVAAEAAAAAEAAVE
                     ASELAKEAQHELDSARALVDQQQRRQQAQNHWDELVALVPEVRRRDREVVLARAAAAF
                     VPLLDLVAEAEVELDDATRTVDEATAAIRRPEADACPDLSSIEAVQALSDSRRRELAR
                     LTELVESQAEVERLMLDLAEVAAVLERSRAREAELVAATSAELAAVDEALLAARESRA
                     DARDGLYDVRERRIAGMAAQLAAELTPGTPCPVCGSVEHPQPATLARDHVSADDEAAS
                     RSRVEVAEDAVAQLEQRRERLHGESTVELESVRATVLTLQAQHAERSAATLARQSRIQ
                     AVVGDTSLDEQIAVVSEQVESADALVSAMRAASDAAVTLRTTVDRLDRLVADSVFATV
                     QEVRDAVRSPADIASAEALNRTHTTERAVVEKMLADPALHRAAQTPAPDIEQLVRTTD
                     ARVGRAAAANARADRLEEAAVRLESLIGDVHGQLARWQPLRGRRDLTEQVAQLVAGTA
                     KDNLSKIRLSHYVLAARLAQVVAAANVRLRGICAGRYELEHTLQRGVGDARGGLGLRV
                     MDTYTGRLRDPATLSGGETFYVSLALALGLADLVNNEIGGTELSTLFVDEGFGSLDAD
                     TLDEVMGELDALRSGGRSVGLVSHLAELRMRVPTQLSVVRSRKGSRLDDS"
     gene            complement(21100..22266)
                     /locus_tag="VH419_17870"
     CDS             complement(21100..22266)
                     /locus_tag="VH419_17870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HEY7045544.1"
                     /translation="MRFLHTSDWHLGRSFHREDLLGAQAAFVDHLVDTVRSERVDAVL
                     VSGDVYDRALPPVDAVELANDTLHRLVSTGVRVVISSGNHDSAQRLGFAADLIDTAGV
                     HLRTDPSRIGTPVVFEDARGNAGGVAVYALPYLEPDLVRTSLDVSERSHQAVLSAAMH
                     RVRANAADHPGRRCVVMAHAFVTGATASQSERDIAVGGVSSVGRDTFAGVDYAALGHL
                     HGRHTLSETVRYSGSPLAYSFSEASHRKGSWLVEMGAHGLESVTFVDAPVPRPLALIR
                     GRFDELLVDRRYDTAEHAWVQVTLTDALRPRDAMEQLRSRFPHCVAILFEPDGQQASM
                     PPDGFDPRHLADRRDEEIVADFVRDVRGREPSTDEAALIRAACEGCRLAEDLAS"
     gene            complement(22583..23431)
                     /locus_tag="VH419_17875"
     CDS             complement(22583..23431)
                     /locus_tag="VH419_17875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020389980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HEY7045545.1"
                     /translation="MNLPRRPGPTSRRFVHEVVDGTARPRPDAVVTEEPLEIRLSWPG
                     QPAHRVAVTMRTPGADFELAAGFLLAEGVVPLCEPPRSVAYCTDVSLAPEQEYNVVTV
                     ELADTPRRHPGSRSTAISSACGVCGTESIDEIFRPDDDPIPVGSLVKADVISSLPDRL
                     GERQRVFSRTGSLHAAGVFDPTGRFVVAREDVGRHNAVDKVLGARQLGSTSYDADVLL
                     CVSGRVGFDIITKAVAGRLGTVVAVGGPSSLALDLAERAGVTVCGFTRGGRFVAYTHP
                     ERIAGI"
     gene            23469..>23953
                     /locus_tag="VH419_17880"
     CDS             23469..>23953
                     /locus_tag="VH419_17880"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEY7045546.1"
                     /translation="MTVPGHPHGAAGGPDHRRRLTVVLLVTSVVVLIQIAGAVVSGSL
                     ALAADAGHMFTDVAAIGIALSAMTIAERSTTARSTFGLYRLEIFAAAVNAVILLAVAG
                     WVVWSAVRRLEDPPEVESGLMILVALIGLLANAFSLWWLREGQASNLNVRGAYLEVLG
                     DL"
BASE COUNT         3783 a         8266 c         8187 g         3717 t
ORIGIN      
        1 gtcgtccaac gccgccacga ggactgggtc agcccaggtc gcgtcggagg cgaagacctg
       61 cgcgctttcg tgcttgaccc ggctctctgc atacatgcgc aacccgaacc ccggctgcag
      121 ccgtaccagt gggtccccga acgcagcttc gctaccgacg ggacgccgac cggcgagctc
      181 accgagcagg cgggcggcgc gagcgaacgt gtccaggctc cacgggccgg tccgctcgtc
      241 cacgtcctcc atccagagcg cggcgtgcag gtcgtcgggc tcgtgcacgg cgtacagctc
      301 gggcatccgc aagcagtcgg gcagcaccga ccggacgggg gagcggagtg catcgatctc
      361 cagccgccac gggaactccc ggacgaagcg gtcgcgcagg tgatcgggaa tgacgtggag
      421 gagctcccag gcgagtggac tctgcagcac cttgacgaat gccgaccagc gagcggtccg
      481 tccgcccacc gtcgcccggc cgacggcccg cagtagcgca cacgtcgaca ccgttccgat
      541 gtcgtacgac accggctgca gcaccgccgt cgctcgatgc aggtcgtcga cggccagcac
      601 tctccgggca acctcgctca gagtggcttc gccgagctgt gcgtactccg ctccggcaac
      661 gacgtcgatc cggcccgcgc cggtcgtcac ggtcattcgt ccccctaccg aagccaggta
      721 gtaaggctac ctgacaagtc ttccaggata gtaaggtagc tttatgaccg tcgtcaaggg
      781 gagtgcgcga ccggtgccgg ggatcgacgc gttcgagccc aagcacatgg gtttcctgct
      841 cgaccggacc atgcgcagga tgcgacacga tctctaccac ctcgcggacg ccgagggcat
      901 catcggtcgc tacgagccgc tccgcccgtc gtccttccgg ttgctgtcgc tcatccctgc
      961 cgggggagcg cgggtcacgg acctcgccgc gcgggccagc atgaccaagc aggcactcgg
     1021 acagttcgtg gacatgctgg agccgttgcg gtacgtcgag agcaaccagg acccggccga
     1081 ccggcgttcc cggatcatca cgcgcacacc cctcggtgac gaggcggtcg ccgacgtcaa
     1141 tcacctgtac gagctgctgg atgaccagtg gcgcgacatc gtgggaccgc ggcgctgggc
     1201 gacgttccgg gccgtgatgc tcgagctcgc gatcggctgg gaggagtcct agcgcgatca
     1261 gcagcgtgcc gcgacgacgc ggccgcgcac gtcaccgagc gtgactcgac caccgtccgg
     1321 accaggcgcc cacccgctga tcgtcacgac gtcgccgtcc tcgaggaagc cccgggtgct
     1381 tccgtcggcg agggtgatgg gctcggtgcc gttccaggtg agctcgagca ggctgccgaa
     1441 cgtcgagcgg tccgctccgc tcaccgtccc ggacgcgaag aggtcgccgg tccgcaggct
     1501 cgccccgttc accgtcaggt gagcaagcat ctgcgccggc gaccagtaca tgtcgcggta
     1561 ctccggagag ctcacgagtg agtcgttgag gcgcacctcc aggtgcaggt ccaggccgaa
     1621 cgtgtcgcgc gccgactcgc cgtgcaggta ggggagcggg gtggggtcct ggcgcagcag
     1681 gtcgaactcg gcctcgccga gggcctcgaa gggtacgacc cacgccgaga tgctcgtcgc
     1741 gaacgacttg ccgaggaacg gcccgagcgg gacgtactcc caggcctgga tgtcgcgtgc
     1801 gctccagtcg ttgaggagca ccactccgaa cagatgacgt cgcgcctgcg ccaccgggac
     1861 gggttcgccg agcaccgtcg cgccggcgac cacgaagccg acctcgcact cgatgtccag
     1921 ccgtgtggag ggcccgaacg gcgggatacc gtcagcaccg ggtcgccgtt gtccgcacgg
     1981 cctgacgacg tcggtgccgc tcaccacgac ggtgccgccg cgcccgtggt agccgatcgg
     2041 gaggtgccgc caggcggccg gaaggtcggg tgcgtcgggg cggaacagct tgccaacgtt
     2101 cgacgcgtgc acctcgctgg cgtagaagtc cacatagtcg gcgacgtcca cgggtagcag
     2161 catcgtcacg gccgacgcgt cgtgaaggtg ctgctcgacg agcccgcgat gcgtgtcgcg
     2221 ggtgaccagg tcgcgtaccc acccgcggat ctcgtgccag tccgacggat ccagcgccat
     2281 cagtgcgttg agcgacgtgg aggcgaacac gccggcgtgc gggtgctcca acacggtcgc
     2341 cacctgcgcg agatcgacga cctgatcgcc cacccgggtt cccacccggg gggtcgagga
     2401 cccttcggta gcgaagacgc cgtagggcag cgtgtcgatc ccgtacgccg agtccgcgct
     2461 cgggctgagc cacgtcatcg tgactccttt tcgacgtcca cgagaccgag atccgccagg
     2521 tcgcgcaccg ggtcctccac gccacagcaa ccgaacgaca cgaaggctcg gcgtacgccg
     2581 gccacgtcct gcgccgacca tgaacgcacc gtctcggcga gcacggctcc gtcgcgttgt
     2641 ccgagcagct tcgccacctc cgcctcggtg ccgcccagca gggccgcgtg tgtcgccgcc
     2701 aacaggttga ggaaaccatg gtgctcgaaa ccggactcct cgtcgcggaa ccgcaccgcg
     2761 tggtgcaagc ccgcggtgta cttcatcggc gcgtcggcga gggctgcggc caccaccacc
     2821 cgagccagcg tcggctcgtc cggaaaagcg tccggcgtcg taccgccggt gcggaacttg
     2881 ccgacgtggc cgggtgcgtc ggcgatcgcg ccgagcaggc tcagtccggt ctcgtcggct
     2941 cgcagctcgc acgcgacgcc gaggccgggc gcgacctgcg gaaggggcag gccgtgcgcc
     3001 ggcagttcga agccgacgac gtgcagtccc ggaaccgcgc cgtgcacctc ggtcccaccg
     3061 atgacgacga cctcgagcgg cggcgaaccg atcgcggcgt gcgcagcgac gacgtcctgc
     3121 catcggtcga cgtgcacgag gagcgggccg acgaacgcgt ccatgaagcc ggaccggtat
     3181 cccaggtgcc gggtgagcgc agtcgtcgcg tcagcgttgc ccggcggaaa catcgcggcg
     3241 tcgtcgagca ggtcgtggaa cagcagctcc ttgctgactc cgcgagagcc caccggcgaa
     3301 agcaccttga gacggtactg cagacgtatg gcactgtggg cacgtggcgc actatcgatc
     3361 tgtgggctct gtgcccccga aacggcatac ccaacatcga cctgacggca cgacgctggt
     3421 gttcgaggag ctgatgggcg aggagggctt ctcgtcggac tcctcgctgc tctaccaccg
     3481 caacctcccg tcgcggatcg tggcggccga ggagtgggcg atgcccgccc tggccaccag
     3541 ggcgaaccac ccgctcaagc cgctgcacct gaagctgcac gacctcttcg atccggagaa
     3601 gatcgacgac atcgacgtcg tgcgcggccg gcggctcgtg ctcggcaacg gcgacgtccg
     3661 gatctcgtac gtcgtggcgg gcgctgcctc gccgctgtac cgcaacggca tcggtgacga
     3721 gtgctgctac gtcgaacgcg gcagcgcgcg cgtcgagacg atcttcggcg cgttcgatgt
     3781 cgcgcagggt gactacgtga tcatcccgcg cgcgacatca caccgctgga tcccctctgg
     3841 cgagggggag ccgctgcggg tgtactgcat cgaggcgaac tcgcacatct cgccgccgaa
     3901 gcggtacctc tcacggttcg gccagctgct cgagcacgcg ccgtactgcg agcgcgacct
     3961 ccggggaccc actggaccgc tgctggccga ggacgtcgag gctgaggccg actccgacac
     4021 cgaggtgtac atcaagcacc gggggaacgg gccgagcggc gtggtgggca cgatcttcac
     4081 ctatccgttc caccccttcg acgtggtcgg gtgggacggc tgcctctacc cgtacgtctt
     4141 caacgtctcc cacttcgagc cgatcaccgg gcgggtgcac cagccgccgc ctgtgcacca
     4201 ggtgttcgag ggatacaact tcgtggtctg caacttcgtg ccgcgcaagg tcgactacca
     4261 ccccctctcg atcccggtgc cgtactacca ctcgaacgtc gacagcgacg agatcatgtt
     4321 ctacgtcgac ggtgactacg aggcgcgcaa gggctccggg atcggccgcg gatcgatcac
     4381 cgtccaccca ggcggccacg cgcacggccc gcagaccggt gcgtacgaga agtccatcgg
     4441 cgtgcactac ttcgacgagc tggcggtgat ggtcgacacg ttccgtccgc tggagctcgg
     4501 agaaggcggc caggccgccg acgacggcag gtacgcgtgg tcgtgggcga ggggaggcca
     4561 ggctgccgag cctccctcgc acggctgatc gacggaccgc agcgtcctga ccgggtggtg
     4621 cggctcacca cgccacccgc ccgttctgcg aggcgacacg tattacagtc gtgtgtggcg
     4681 accgagggag tcgcttggat cgtcatgagt ggaggcgtct gtgccggagc gcgggccggg
     4741 ccggctgcca gaccaggtcc agggagcacc ccgggccgaa gaagaccacg agcccagcgg
     4801 gagcaacgac gccgacagca ccggccggaa ggctcagcag ccggagctgg tgagcaaccg
     4861 cgtcctcacc gtgcccaacg cgttgagcat cgtccgactg tgcggtgtgc cgctgttcct
     4921 ctggctcgtc ctcgggccgc agttggacgg ctgggcgctg ctggtgctcg ccgtgtccgg
     4981 catcaccgac tggctcgacg gcaagctcgc ccgcacgctc aaccagtcat ccaggctcgg
     5041 ccagatcctc gacccggtgg ccgatcgctg ttacatcctg gcggtggtga tcgggctggc
     5101 gctgcgcgac atcatcccgt ggtggctcgc tgtcggtctg ccgttgcgtg acgtcttctt
     5161 gttctgtctg gtgccgttcc tgcgtacccg cggttacagc gcgctgccgg tgcacttcct
     5221 cggcaaggcc gccaccgcct gcttgctcta cgcgttccca ctcctgctgc tcggcgacgg
     5281 cgacagcacg ctggcgcgac tggcggacgt cttcggctgg gcattcgcga tctggggaac
     5341 cgcgctgtac tggtgggcgg gcatcctgta cgcgtaccag gtcgtgcagc tgatgcggac
     5401 gcctcgagtc gccgaagagg tcagatgagc gacgaggaac catcgacgtc aacccctccg
     5461 gtctcgcagc aaccccgtgc gtacgtcggt ctgctcaccg aggtgatgag caacaccctc
     5521 gacgaggact acgccaccgt cgctgcgaag cgtccgtccg gtccggtcga gagtccccgc
     5581 gggcggcgta cgcggaacaa caggatcgcg ctgacctcgg tcgtggtggc gttcggcctc
     5641 atgctgggga tctccgcctt gaagacggac aaggaccggc cggtgacacg tgccgagcga
     5701 gacgagctcg tcgcgcagat ccacaaccgg cagcacgaac aggcacagct ggctgctcag
     5761 ctcaacgcag tccaggacga cgtgacggat ctccagaacc gggtcggcac cgaggcctcg
     5821 aggaactcac agatcaccga ccagctcgtg gcacttggcg tgcaggcggg cacgctcgcc
     5881 gtcgagggtc ctggcatggt gatcacggtc gacgatgcgc catcggccga cgagggatca
     5941 ggcggcacga tcctcgacac cgacctccaa gccctcgtca acggactctg ggcagccggt
     6001 gcggaagcgg tgtccatcga cggccatcgg ctcacgtcgc tgaccgcgat ccggttcgcc
     6061 ggttcggcca tcaccgtgga caacgtgtcc ctcgccccgc cctacgtcat caccgcgatc
     6121 ggcaatccgg atacccttcc ggcacggctg ctggagaccg agggcggcca gacctggttg
     6181 ggactcgaag ccaacttcgg catcaggttc gaccgcgaga cgaaggagaa cgtcgtcgtg
     6241 ccgggggacc cgcacgatca cctgctgtac gccgagccgg acaccggcgg gtgatcgacc
     6301 cgtgatcgcc gttttcggcc tcgtcgtcgg catcctggcc gggctgttcc tcgagccgtc
     6361 ggtgccggca tcgctccagc cctatctccc catcgcggtc gtggcggcgc tggacgccgt
     6421 gttcggcggg ctgcgggcgt tcctcgacgg gatcttcgac gacaaggtgt tcgttgtctc
     6481 gttcgtgtcg aacgtgctca tcgccgcact catcgtctat ctcggcgacc agctcggtgt
     6541 cggcggccag ctgtcgaccg gcgtgatcgt cgtcctcggt atccgcatct tctccaacgt
     6601 ggccgccatt cgccgccacc tgttccacgc gtgagtccga tgagcagcga caacgccgac
     6661 cgcgacgacc gggatcccgg gtctgaggaa accatggatt ctggcggcgc atccgggcgg
     6721 cagcggttgg cggacgcgct tcgcgcccgt ccctcgcggg cccagttcgt ggtggcgttg
     6781 ctgctgtgcg ccctcgggat cgcggcggcg acccagatcc ggttgacgca ctccgacgac
     6841 gacttcgccg gccagcgacg cgaggacctg gtcgacctgc tcgactcctt gtcgtccgcg
     6901 accgaccggg cgcgcacaca gctcgacgac ctcgaacaga cccgcaacga gctgcaatcg
     6961 agctcggctc gccgcgcggc agtgctcgag gaggggcagc agcgactggc agtgctcggc
     7021 atcctcagcg gcgcggtcgc cgccaccggc ccgggtgtga cgatcacgat cgacgacccg
     7081 cgcggttcgg tgcaggcagc caccctgctc gacgggatcg aggagctgcg cgacgccggc
     7141 gccgaggcca tcgagatcaa cgacagcgtt cgcgtggtcg caacgacctc gttcatcgac
     7201 ggcgacgggg agatcatcgt cgacggcgag acactccgac cgccatacgt gatcgatgcg
     7261 atcggcgcat cgcacacgct gagcgaggcc gtcgtctttc cgggcgggct gtcggacgac
     7321 gtcgagcggg acggcggaac ggtcacggtc aaggaggcgg acgtggtcga ggtcggatcg
     7381 ttgcatccga tcgagcagcc tcagtacgct cagccgacca acaggtaacg cgccggccga
     7441 aggagcctga cgatggccta ccccgacgac ctcaagtaca ccgccgaaca cgagtgggtg
     7501 aaagcagggg aggggtcaac ggtccgggtc gggatcaccg actacgccca ggaggcgctg
     7561 ggcgacatcg tctacgtcaa cctccctgac gtcgacacga cggtcgaggc cgggagcgcg
     7621 atgggtgagc tcgagtcgac caagagcgtg agcgacctgt tcgctcccgt gtcgggcaag
     7681 gtcacagcgc gcaatgaagc gttggacgcc aaccccgagc tgtgcaactc cgacccgtac
     7741 ggcgaaggct ggatcgtcga gatcgatgcc gacgacgcgt cggcggtgga aggcctgatg
     7801 tccgccgacg actacgaagc cacggtaacc tccaactagc gcccgctccc ggtcgagacg
     7861 ccctgggtaa ccttcacgtc gatggtgagg tttcgtgccg agactgcccg cgacgcgggg
     7921 gtccggagga ggaagacatg ccgttttgca ccgaatgcgg tcatcagaac cctgatggcg
     7981 ctcggttctg ctccaactgc ggtcaccggc tcgtggccac ggaagcacca gccgaatcga
     8041 cctcgacgtt gagcttcggt ggcccggacc gcagtgaacc gcagtcgacc ggcttgtccg
     8101 atgccgacga ggcagccgtg aacgccctgc cccgcggcag cgcgctgctc gtcgtccagc
     8161 gtgggtcgag cgcgggcagc aggttcctgc tcgactccga cgaggtgacc gcagggcggc
     8221 atcccgacag cgagatcttc ctcgacgacg tcacggtgag tcgccgccac gcggtctttc
     8281 gtcgtacgtc ggacgggtac ctcgtcgccg acgtgggcag cctcaacggc acctacgtca
     8341 accgcgaccg gatcgacgag gtgttgctcg tcactggcga cgaggtgcag atcggcaagt
     8401 accgtctcgt gtacttcaac agcccgcagg acaccgacac gtgagctcgg cgacagccac
     8461 cagcggccga gggcggatga gcatcggcga ggtcctcaac cgtctgcgca gcgactttcc
     8521 cgatgtgagc atctcgaaga tacggtttct cgagtccgag gggctgatcg agccggagcg
     8581 gaccccgtcg ggataccgga agttctccta cggcgacctc gagcggctgc gctacatcct
     8641 ctccgagcag cgcgaccact actacccgct caaggtcatc aaggaacacc tcgacgcgat
     8701 ggagcgtggc ctggagccgc cggccgtggc cggtgagtcg ccacgggcgc cgcactccgt
     8761 gcgctcactc ggcatcgtca ccgaggacgc gagcgacttc ggcccggccc cagccagcga
     8821 tctgcggatg tcgcgagccg agctgctcgc caactccggg ctcaccgacc agcagctcac
     8881 cgagctgatg gagttcggga tgctccgtcc gcgccctggg acccgctact tcgacgcgga
     8941 cgccctggtg gtcgcggaga cgatcgccca gctcacggcc ttcgggctcg aagcgcgcca
     9001 cattcgcgcg ttcaaggccg ccgccgaccg cgaggtcggg ctcatcgacc agatcgtcag
     9061 cccgctctcg cgtggccgcg acgacaacgc tgcggcccgc gccgccgaca ccgccgccga
     9121 gctcggcgcg ctctccgttc gcctgcacgc cgccttggtc aagaccggcc tccgccgccc
     9181 ccacccccgc tgaccgacct cccgccaacc tcccggcgat gcccggtcga agtggcaact
     9241 ttcgttgagg tttggcggcg tgtcgaacga cgatcgccac ctcggcgcat cttgggcgag
     9301 ggccgcgaga gagtacgctg aaaacgtgcg tgaggtcgat gtgatcggag tccgggtcga
     9361 gatgccgtcg aaccagccgc tcgtgttgtt gcgcgagagt tcgggggagc gctacctgcc
     9421 gatctgggtc ggagccgtgg aggccacggc catcgccttc gcacagcaag gagtggttcc
     9481 tccgcgtccg ctgacccacg acctgatgaa ggatctgctc gtggcgaccg gccacgagct
     9541 gcacgaagta cagatcaccg aagtacgcga cggcatcttc tacgcgatcc tggtcctcgg
     9601 cgacggcgtc gaggtcagcg cccggccgtc ggactcgatc gcccttgccc tgcgcaccgg
     9661 gagccgcatc gtctgcagcg acgaggtgct cgaggaagcc ggcatggcgg tccccgacga
     9721 ccaggaggaa gaggtcgaga agttccggga gttcctcgat cagatcaacc ccgaggactt
     9781 cgggccgtat caggggcagc gcggcgagac cgagaccggc gagtaacccc tcggccgtca
     9841 gaaaccctca acctcaacct ctagttgaga gttgcgacac gccctcggcg atcgcccgaa
     9901 gtcgttgacc ctgccaccag ccgcgccgta ccgtctcccc tgaagaccct cgctgtaact
     9961 tcaccgctgg cacttccccc gggcagcgag ctgagaccag gaggtcccgt gtctggagac
    10021 aaggacaacg cagcctcgac ggcgcgtgag gccaacgcgg cggccgaggt ggctgcggcg
    10081 atcgacactg cgagcgggca aggtctgctg ttcgacgacg acgtcgcgcc catgccagac
    10141 gacgttggct accgcggtcc gatggcgtgt gctgctgccg gcatcacgta tcgccagctc
    10201 gactactggg cccgtaccgg cctggtggag cccacggtcc gcacggccgg cggctccggc
    10261 acccagcggc tctactcctt ccgcgacatc ctgctgctca aggtcatcaa gcgcctgctc
    10321 gacgccgggg tctcgctgca gcagattcgc agcgccgtcc accacctccg cgagcgtggc
    10381 accgacgacc tgaccgaggt cacgctgatg agcgacggcg ccagcgtgta cgagtgccga
    10441 tccgccgaag aggtcatcga cctcgtgcaa ggcggacagg gcgtgttcgg catcgcgatc
    10501 ggcggtgtct ggcgggagat cgaaggatcc ttgtccgagc tgccgagcga acgcgccgcg
    10561 cgcgacgacc tcgagcaccc tggcgatgag ctggcagcca ggaggcgcgc ccgccacgct
    10621 ggttgacagc gcgttcgggg ctggcgttgc gcagacatgc gcaacccgcc ggcgctggta
    10681 aggttgcgaa tgccgcaaca gtcacacggg agagtccgag actcgcacac actcgtacga
    10741 cggatcggcg ccgaaggggc aactcctccc cggaacctct caggcatcag gaccgtgtcg
    10801 accggcaact ctggagggtg cgcgccaccc gcgtcctgac agaggggagg ctcgatgcgt
    10861 ccgacgccga aggagccttc cgatgaccga ccggcagccc attcccacct tgcgcgaact
    10921 cgagtccgcc gcgccgttct cccgtcgcca catagggcct gatgcggccg agcagcgtca
    10981 catgctggac acgctgggat tcgccggcat ggaggacctg ctgtacgccg cgctgcccga
    11041 caagatccgc accgacaagc ccctcgacct tcccccggcg ctgagcgagg tcggcgcccg
    11101 cgacgcgctc ggcgcgatcg ccgcgcgcaa caacgtcaag gtcccgatga tcgggctcgg
    11161 gtactccggc actgtcacac ctgcggtcat ccgccgcaac gtcctcgagg acccgtcctg
    11221 gtacaccgcc tacacgccgt accagccgga gatctcacag ggtcggctgg aggccctgct
    11281 gaacttccag accatggtgg ccgacctctc cggtctcccg atcgcgaacg cctcgctgct
    11341 cgacgaggcg actgcggcgg ccgaggcgat gacgctcgta cgccgcgcca accggtccaa
    11401 cgcgtcagcg cgtttcgtcg tcgacgagca ggcgctgccg cagaccttgg cggtcgtgcg
    11461 cacccgggcc gaggcgatgg gtatcaccgt cgacgtgcgc gacgtctacg ccggactacc
    11521 gtctgacgac ctggcaggtg tcctgatcca gtacccggat gcacacgggc gggtacgcga
    11581 cccccgaccg gtgatcgagg cggcccacgc cgccggtgcg ctcgccgtgg tcgccgcgga
    11641 tctgctttcg ctgacgctgc tcgaggcgcc cggagagctg ggtgccgatg tcgtgctcgg
    11701 ttcgagccag cgtttcggcg taccgctctt ctacggcggc ccacacgccg ggttcatggc
    11761 ggtccggacc ggactcgagc ggcatctgcc cggccggttg gtcggcgtct cggtcgacgc
    11821 cgagggccgg ccggcgtacc gactcgcact gcagacgcgc gagcagcaca tccgccgcga
    11881 caaggcaacg tcgaacatct gcaccgcaca ggtgctgctc gccgtggcgg cctcgatgta
    11941 tgccgtctac cacggtccga ccgggctcac ccggatcgcg cgacggacac accgttacgc
    12001 aaccgtactg gcggaggcgt tgcggcagac cggtcaagag gtcgagcatg cgcacttctt
    12061 cgacacggtc agtgtccgcg tcgatggtga ggccgacgcg atccgggtcc gggctggaga
    12121 cctcggcctg cacgtcctgg ccgacggtgc cgaccgggtc ggcgtctccc tttcagagct
    12181 caccacgcgg gagcacctcg ccctggtgct ggacgccttc gacgttcgcc ccgactcggt
    12241 ggactgggac gcgctggaca gctcggccaa cgaccccatc ccggaggagc tgcgccgccg
    12301 tacgccgttc ctgacgcacc ccgtcttcaa cgaccaccgc tccgagaccg acctgcttcg
    12361 ctacctcaag cggctctccg accgtgacta cgcactcgac cgcggcatga tcccgctcgg
    12421 atcgtgcacg atgaagctga acgcgacggc cgagatggag ccgatcagcc ttcctggctt
    12481 cgccgacctg cacccgttcg tcccggcaga ggacgcgctc ggctatgtcg agctggtcga
    12541 gagcctggag cgctggctcg ccgaggtgac cgggtacgcc gaggtgtcga tccaaccgaa
    12601 cgccggatcc cagggcgagc tggccgggct gctggcgatt cgtggctacc accgggcacg
    12661 tggtgagacg ggacgagacg tctgcctgat cccgtccagc gcccacggca ccaacgccgc
    12721 gtcggcggtg atggctggga tgcgggtcgt ggtcgtgaaa gccaccgacg aaggcgcgat
    12781 cgacctcgac gacctccggg ctcggtgtga gacgcacacc gacgaactgg cggcgatcat
    12841 ggtcacctat ccgtcgacgc acggcgcgta cgaggacacg atcaccgagc tgtgctcgat
    12901 cgtgcacggc catggtggcc aggtgtacgt cgacggggcc aacctcaacg cactgctggg
    12961 tctggcgaag ccgggcgagt tcggtgggga cgtctcgcac ctcaacctgc acaagacgtt
    13021 ctgcatcccg cacggtgggg gcggacctgg cgtcggcccg gtggcggtcg ccgcccacct
    13081 ggcgccgttc ctcccgagcc atccgctgca tcctgagcgt ggccgtcgcg acgggatcgg
    13141 accggtgagc gcggcgccgt acggctcggc cggcatcctg ccgatctcgt gggcgtacat
    13201 cgcgatgatg ggccaggccg gtctcatcga ggcgacgacg gtggcggtgc tctcggccaa
    13261 ctacatcgcg cgtcgtctcg cggagcacta cccggtgctc tacaaagggc acggcgacct
    13321 ggtcgcgcac gagtgcatcc tcgacctgcg tcagctgacc aaggccagcg gtgtgagcgt
    13381 cgacgatgtc gccaagcggc tgatcgacta cggcttccat gcgccgacga tgagtttccc
    13441 ggtcccgggc acgttcatgg tggagccgac ggagtcggag aacctcgccg agctggaccg
    13501 gttcatcgac gcgatgatcg ccatccgcgc cgagatcgcc gacgtggagg cgggccgcac
    13561 cgatctgtcg tcgagcccac tcgtccacgc accgcacacc accgccgcgg tgacgggcga
    13621 ctgggaacgc agctactcac gggcggtcgg tgcgttcccg gccggacact ccgcggacaa
    13681 gtactggcca ccggtggccc ggatcgacca ggcgtacggc gaccggcacc tggtgtgttc
    13741 ctgccccgac ccgtcggcgt tcgccgagga cggttcgtga ccgaggaggc caccggagac
    13801 cgccggtcgg tgctggtcgc tgacgcgcac acgaaaggac ggttgcctgg aatcgtcgcc
    13861 gcgacctcgc gggacggcgt cgtcgattgg cgctgcgtgt tcggcgacac cggggcggcg
    13921 taccggatcg cctcgatcac gaagacgttc actgctgtcg ccgtgatgca gctgcgcgac
    13981 gaggggcgca tcgacctcga tgaggcgatc ggccggcatg tgcctgagac cccgtacgcc
    14041 gaccaggcca tccgtcggct gctgtcgcac tcctcaggga tgacggccga gccggccggc
    14101 ccctggtggg agcgggtgcc gggcggcagc tggagcgagc tcgtcgccgc gaaccggcga
    14161 gcgacccacg tcttcaagcc gggggagcgc taccactact ccaacctcgg ttacggccta
    14221 ctcggtgaac tggtcgcgcg gttgcgcggc cggagctggt gggacgtcgt gaccgagcgc
    14281 atcgtcgagc cggtcggtct gacgggtacc acgttcgccc gaccggacga cgcggcggtc
    14341 ggcacctcgc gcaacccact caccgacgag ctcgtccgtg agcccagcga ggacgagggc
    14401 gcgatggccg cggcaggtca gctgtggagc acgcccgacg atctgcttcg ctgggtggac
    14461 gtgctcgccg ccggtcatcc gggcgtgttg tcggccgaca ccgccgtgga gatgcgcacg
    14521 gtccagtccg gtgacccgga cctgcagcat cgcggtgcct acggactcgg cctgcggttg
    14581 cggtggtctg accaggcgac gttgatcggt cacagcggct cactgcccgg cttccttgct
    14641 gggatgttcg ccgatcccgt gtccagggtc ggcgcggtcg tgctcaccga cgcgacgtac
    14701 ggccttgacc cagagggtgt ctgtgcgtcc ctgatcgagt cggcgccgcc agcgcaggcg
    14761 cagccgccgg tcgccgaccc cgcctcacct gccaccgacc ttgccggggc gtggtactgg
    14821 ggaaacgtgc cgttcgagct cgtcccgacc gcgtcaggtg ccatcgtcat cagcggcgcc
    14881 aaccggtcgc ggatcgagca ggtcggtgtc gatgtctacc gtggactcaa tggttactgg
    14941 gccggtgagc tgctccacgt gcaccgggat gccgacgggc gggcccggca tctcgacgtg
    15001 gtgacgttca tcctcacccg aacgccatac gatccgaagg cgcccattcc cggccggact
    15061 cccgagccac tgtgaggcag accagccggt cgaggtggca ctcgtggcgc gacacgccgg
    15121 cagagttcaa caaaacctgc cacctcggcg aagggcgtca gctggcttgg ctgacgctgg
    15181 acatattgaa gtcggggagg cgaaggggcg gcgtcgcggt gcgggggaag tggtcgcccc
    15241 actcgcggga gaagcttcgc acggtctcgc ctgcccgcga gaaccggttc aacaggtcga
    15301 tcggtgactc gttgaatcgg aagttgttga cagcaccgac cacctcgcca ccttcgacga
    15361 ggtagacccc gtcccgcgtc agacccgtga gcaagagcgt ctgggggtcg acgcttcgga
    15421 tgtaccacag gcacgtcacc agcagcccgc gatcgacctc ggccacgacg tcgtccaggg
    15481 cgccatgccc ggaggcgatc tccaggctga ggttgtcgat gagcggtgcc accggaagcc
    15541 cggtctggtc cgcggtgtgg cgggacgtgc cgagatggcg gagcgtcccg tcggcgatcc
    15601 agtccgtctt cgccagcgtc aagccgttgt cgaagacgct ctgtgcggac gacgtcgcgc
    15661 gcgcggtgag gaacggtgcc gcagcgaggc cgggagtcgc cgggtcggag gacagccgta
    15721 cgccggtcgc cgcgatccgc tgcccgacct tggtgccacc gtccgggtcg ctgtagacgg
    15781 tccgcccgtc gtacgcgtcc tgcccgctcg ccacgaagta cgcatagatc atcaggtctg
    15841 cgacggcggt gggaggcagc acggtgtcgt agcgtccggc cggcagctcc accttccgcg
    15901 tcgcccagct gatccggcgt acgagttccg cgtcgagtgc cgacaggtcg atctcgtcga
    15961 tccgttcggc agcctggccg acccacgcgg acgtggtcag gtcgcccggc ttgccggtga
    16021 tgccgatgtg cccggtgggc tgctcgtgcc gaagacgcag gccggtcgag gtggccaggt
    16081 aggtggtcgt gacgtcgtgc tcgacgtacc cgaagagcag ccggtcgtcg tcgcgggcgc
    16141 ggccgagtac gtcgccgagc gcgaccgcga agtcctcgaa cacgtcgata ctggtcgtcc
    16201 cggcaggatc gtcccacgac gaggcagcgt ccccgttgac gagcggccgg gcgtcgtctg
    16261 ccggcgaggc tgccctggcg gtcgcgtcgg cgcgggccac caggtcactc acctggccag
    16321 gggtcgacgc ggactgcgtc agcactccgg cgctgacgcc ctgcctcccg tccacgacgg
    16381 agaccacggt caggtgactc gccgtcatca cgccgttcgt cgtcagcgtg ttgttggccc
    16441 acctgaggtt ggcggtggag gacagcgtca ggatgaccgc gcaatggtcg gccgtcgaga
    16501 cgtcgagtgc gtgctcgacg agggcctggg gcgtcgccat catcgtcctg cctcgtgggt
    16561 tgtgttcaag acgttgacgt cacggaacag cgcggaagga caaccgtggc tcaccggcgc
    16621 cacttgtccc ggttgcgcct tgccacagtt gaaggcgccg ccgagcaccc aggtgtcagc
    16681 gccaccgacg gcttccatgc tgccccagaa atccgtcgtc gtggcctggt aggcgacatc
    16741 gcggagcggg tgcgagaggg tgccgttctc gatcgcgaag aaccgctgac cggtgaactg
    16801 gaagttgaac cgttgcatgt cgatcgacca tgacttgtcg ccgacgatgt agatgccgcg
    16861 ctcgacctgg gagatcaggt cctcggtcga gatgtcttcg ggtgaatgcg ccagcgagac
    16921 gtttgccatc cgctggatcg gaatgtgacc gggcgagtcg gcgaacgcac accccgtcga
    16981 tcgttcccac cccatcctgg ccgccatccg gcggtccgtc tggtagccca cgaggatgcc
    17041 gtccttgacg atgtcccagg cttgcgtccc gacgccgtcg tcgtcgaagc cgatgcttgc
    17101 gagcccgtgc tcgacggtgc ggtcaccggt gatgttgaga tggcgcgagc cgtactcgag
    17161 gctgccgagc ttgtcgatgg tggcgaacga cgtgcctgcg tagttggcct cgtagcccag
    17221 tgcgcggtcg agctccgttg cgtgcccgat gctctcgtgg atggtcagcc acaggttgct
    17281 ggggtggatg acgaggtcga agcgcccggc gtcgaccgac ggtgcggcga gcttggccga
    17341 cagcaggtcg ggcagctcgg caagctcggt gtcccagtcg tacgcgccgt cgatgaggta
    17401 ctcccagccg cgtccggtcg gtggagcgat cgtccgcatc ccgtccagca gcccacgatc
    17461 gtggtcggca ccgaacacct cgaactgggg gttgatccgg acgcgttgct gcgtcgtcgt
    17521 cgtcccgtgg ctgtctgcgt agaacttgtt ctcccgcact tgctgaacgg tggccctgac
    17581 gtggtcgacc gacggcgccc ccagcagccg ctggctccac tcggccagca cggcgacctt
    17641 gtcaggtgta ctgacctcga acgggtcgac gtcgtacgac gagacccagg tggcatcggc
    17701 gtacaccgcc tcgggcgcga gctcgatgcg ttcgtgggtg atggccttcg cgaccatggc
    17761 cacgcgcacg gcctgctcgg tgacccggac gacatcttcc acggacaggg tgacgccggc
    17821 cgcgaagccc caggtgccgt cgtgcacgac tctgaccgcg aagccgacgt cgtcaccttc
    17881 ggaggcgtcg acgagcccgc cgtcacgtag ctgtaggtgc tgcgtgcgca gccgctcgac
    17941 ccggaggtcg gcgtgctcga ccttcaggtc ctgggcgcgc tgcagcgcgg tggaggcaag
    18001 tcgcgcgagg gggaggtctg cgaactcgag gtcgatgccg tggctcgtgg gcatgccacg
    18061 cagcctatga cgtcaggaga cgaggtggcc gaacacctcg gggtgcgtgt cggtcggtcg
    18121 gtgcggggcg atcacgtcgc cgttgggaag cagctcgccg gtgtcctcga acaggaggac
    18181 gccgttgcag agcagactcc agccctgctc agggtgggcg gccacggtgt gcgcagcctc
    18241 acggtcagcc gcctccgccg acggacaaag gggctcgtgg gtgcaggtcg accctgagat
    18301 aacgcttctc acgtctccag tgtgcatagc gacctcgaaa tcgtcaggag attattggcc
    18361 cgttttcagg aatcatccaa acgggaaccc ttccgtgacc gaacgaccga tagctgcgtg
    18421 gggactcgca tccgcagctc ggccaggtga ctgaccaggc cgaccgagcg gccgccgctg
    18481 cgcagcgcgt cgagctcgcc catcacctcg tccagtgtgt cggcgtcgag actcccgaac
    18541 ccctcgtcga cgaaaagcgt ggacaactcg gtgccgccga tctcgttgtt gacgaggtcg
    18601 gcgaggccga gtgccagggc caggctgacg tagaacgtct cgccgccgga cagcgtcgct
    18661 gggtcgcgca gccgacccgt ataggtgtcc atgacccgga gccccaaccc gccacgcgcg
    18721 tcaccgacgc cgcgttgcaa cgtgtgctcc agctcgtacc gtccggcgca gatgccccga
    18781 agccgcacgt tggcggcggc gacgacctga gccagccggg cggcgaggac gtagtgcgac
    18841 aacctgatct tggacaggtt gtccttggcc gttccggcca cgagctgagc gacctgttcg
    18901 gtgaggtcac ggcgtccgcg cagtggctgc cagcgtgcaa gctgcccgtg tacgtcaccg
    18961 atcaacgact ccaaccggac cgcggcctcc tcgaggcggt ccgcgcgagc gttggccgct
    19021 gcggcacggc cgacccttgc gtcggtcgta cggacaagct gctcgatgtc aggcgccggc
    19081 gtctgcgcag cccggtggag agcggggtcg gccagcatct tctcgacgac ggcgcgttcg
    19141 gtcgtgtggg tacggttgag cgcttctgct gacgcgatgt cagccgggct ccggaccgca
    19201 tctcgaacct cctgcacggt cgcgaacacc gagtcggcga cgagccggtc gagtcgatcc
    19261 acggtcgtgc gcagcgtgac agctgcatcc gacgccgctc gcatcgccga caccagcgcg
    19321 tcagccgact caacctgctc gctgacgacg gcgatctgct cgtcgagact ggtgtcgccg
    19381 acgacggcct gaatccgaga ctgtcgcgcc agcgtcgccg cggagcgctc ggcatgttgg
    19441 gcctgcaacg tgagcacggt cgcgcgaacg gactcgagct cgacggtgga ttcgccgtgc
    19501 agtcgttcgc gccgctgctc gagctgggcg accgcgtcct cggcaacctc gacccgcgac
    19561 cggctcgctg cttcgtcgtc ggccgagacg tgatcgcggg cgagggttgc gggttgcgga
    19621 tgctcgaccg aaccacagac cgggcacggc gtgcccggcg tcagctcggc ggcgagctgt
    19681 gcggccatgc cggcgatgcg tcgctcgcgg acgtcgtaca gcccgtctcg agcatccgcc
    19741 cgggactccc tggccgcgag cagcgcctcg tcgactgcag cgagctcggc cgacgtcgcg
    19801 gcgacgagct cggcttctcg cgcccgggac cgctcgagta cggccgcgac ctcggccagg
    19861 tcgagcatca accgctccac ctccgcttga ctctcgacga gctcggtcag ccgcgccagc
    19921 tcgcggcggc gactgtcgga cagcgcctga accgcttcga tcgacgagag atcggggcag
    19981 gcgtcggcct cgggccgtct gatggccgcc gtcgcctcat caaccgtgcg ggtggcatcg
    20041 tcgagctcga cctcggcttc cgcgacgagg tcgagcaacg ggacgaatgc tgcggctgcc
    20101 cgggccaaca ccacctctcg gtctcgccga cgtacctccg ggacgagcgc gaccagctcg
    20161 tcccagtggt tctgagcctg ttggcgtcgc tgctgttggt cgacgagcgc gcgagcgctg
    20221 tcgagctcgt gctgcgcctc cttggcgagc tccgacgcct cgaccgctgc ctcagccgcg
    20281 gccgcggctt cggcagcaac cacctcccga cgcgcctgga gtcgctcgac catcgcggcg
    20341 tagtcgtgct catcggcctg gtcatgggtg tgcgtgtcgg cggcgtggcc aacgtccttc
    20401 gctgccgtag gggcgccggc gaaggcctcc tcgacccgag ccagcaagcg gtccatggtg
    20461 tgctcgaccg cacgacactc ggcgtccagc gcgcgccggc tgtcggtcaa ccatcgctcg
    20521 actgcgttga agcggtgggt gcggaacaac gtttcgagga cgtcgtgccg atccttcgcc
    20581 cctgctgtga ggaacgtctg gaactcaccc tgcggcagca tgacgacctg gcagaactgg
    20641 tccttgttca tcccgaggat cgacgacacg aggtgaccga cctcgtcgac gcgcgtgctg
    20701 tgcacgtgcc agccgccctc gacgtactcc tcgacggaag ccttcgccgt ctcctcgacc
    20761 acgccggatc ttgcgcggcg cgatggccgg gtccaggccg gctggcggcg cagccgaagt
    20821 cgcctgccgc gcacggtgac ctccagcaga accgatggga cagtggcggg ggcggcatgg
    20881 tggctcttga acgacttggc cgcgttgcgg ttgcccggaa cagcgccgta cagcgcgaag
    20941 cacacggcgt ccagcacact ggtcttgccc gatccggtcg ggccggtgag caggaagaga
    21001 cccgcgtcgt tgagtcggtc gaagtcgacg acttcggtgc cagggaacgg cccgaacgcc
    21061 gtcagctcca gtcgatggat cctcatcgag tggccgccgt cacgacgcca ggtcctcggc
    21121 cagtcgacag ccctcgcagg ccgctcggat caacgccgcc tcgtcggtgg acggctcacg
    21181 ccctcgcacg tcgcggacga agtcggccac gatctcctcg tcacgacgat cggcgaggtg
    21241 gcgggggtcg aaaccgtcgg gcggcatcga tgcctgctgg ccatccggct cgaacaggat
    21301 ggccacgcag tgcgggaacc gtgaacggag ctgctccatc gcatcgcgcg ggcgcagtgc
    21361 atcggtgagg gtcacctgca cccatgcgtg ctccgctgtg tcgtaccggc gatcgacgag
    21421 cagctcgtcg aaacgaccac gaatcagcgc caacggtcga gggaccggcg catccacgaa
    21481 cgtgaccgac tcgagcccgt gcgcccccat ctcgacgagc cagctgccct tgcggtgcga
    21541 cgcctccgag aaggagtagg cgagcggcga ccccgaatac cgcacggtct ccgacaaggt
    21601 gtgccggccg tggagatgtc cgagagcggc gtagtcgacg ccggcgaacg tgtcccggcc
    21661 gacactcgag acacctccga cggcgatgtc gcgctcactc tgcgaggccg tcgcccccgt
    21721 cacgaaggcg tgtgccatga cgacacaccg gcggccggga tgatcggcgg cgttggcgcg
    21781 aacccggtgc atcgcggccg acagcaccgc ctggtgactg cgctcggaga cgtcgagcga
    21841 cgtgcgcacg aggtctggct cgaggtaggg gagggcgtag acggccacgc cgccagcgtt
    21901 gccgcgcgcg tcctcgaaca cgaccggcgt accgatgcgg ctcgggtcgg tacggagatg
    21961 gacgccggcg gtgtcgatga ggtcggcggc aaagcccagc cgctgcgcgg agtcatggtt
    22021 gccgctggag atgacgacgc gtacgccggt cgacaccaac cggtgaagcg tgtcgttcgc
    22081 gagctcgacg gcgtcgaccg gtggcagcgc ccggtcgtac acgtcaccgc tcaccagcac
    22141 cgcgtcgacc cgctcggacc ggacggtgtc gaccaggtgg tcgacgaacg ccgcctgcgc
    22201 gcccagcagg tcctcgcgat ggaaggaccg gccgaggtgc cagtcggagg tgtgcaggaa
    22261 gcgcatgcga gaacggtagg tctcgagccc gacagttcgg cgttaccacg ccgcacccgt
    22321 cgcagcgcca cgtcagatgc cggcgaggcg ttcgctgccg ctgatcgagc gtgtcgagac
    22381 caccacgcgg tccgacaacg tcgacccgtg gccgtcagat ctcggcaatg cgttcgctgc
    22441 cgctgatcga gcgtgtcgac atcaccacac cgtccgagaa cccgaaccgt ggcacgtcag
    22501 ctcccggcaa cgcgttcact gccgctgatc gagcctgtcg agatcactgc acggttggac
    22561 aacaacgtcg acccgtggcg cgtcagatgc cggcgatgcg ttccgggtgc gtgtaggcga
    22621 cgaaccgtcc gccgcgcgtg aagccgcaca ccgtgacgcc cgcccgctct gccaggtcga
    22681 gggcaaggct cgacgggcca ccgaccgcga ccacggttcc gagccgtcct gcgaccgcct
    22741 tcgtgatgat gtcgaatccg acccgtccgc tcacacacag caagacgtcg gcgtcgtacg
    22801 acgtggagcc caactgccgg gcgccgagca ccttgtcgac ggcgttgtgt cggccgacgt
    22861 cctcgcgtgc gacgacgaac cgaccggtcg ggtcgaagac gcccgccgcg tgcagcgacc
    22921 cggtccggga gaagacccgt tgccgctcgc ccagccggtc ggggagcgag ctgatcacat
    22981 ccgccttgac cagcgagccg accgggattg gatcgtcgtc gggccggaag atctcgtcga
    23041 tgctctcggt tccgcagacg ccgcacgcgg aggagatcgc cgtcgagcga cttcccggat
    23101 gccggcgtgg ggtgtccgcc agctccaccg tcacgacgtt gtactcctgc tccggcgcca
    23161 aggagacgtc ggtgcagtac gccacgcttc gtggcggctc gcacaagggc acgacgcctt
    23221 cggcgagcag gaagccggcc gcgagctcga agtcagctcc tggggtgcgc atggtgaccg
    23281 cgacgcggtg ggccggctga cctggccagc tgagcctgat ctcgagcggc tcctccgtga
    23341 ccacggcgtc cggtctcggc cgcgcggtcc cgtcgaccac ctcgtgaacg aagcgccgcg
    23401 aggtcggtcc gggacgtcgc gggaggttca cccttcgagc gtagtccggt ctctagagtt
    23461 gacttctgat gaccgtgccc ggacacccac acggcgcggc tggtggcccg gaccaccgcc
    23521 gccgcctgac tgtcgtcctt ctcgtcacca gcgtcgtcgt actgatccag atcgccggcg
    23581 cggtcgtcag tggcagcctt gcgctcgcag cagacgccgg ccacatgttc accgacgtgg
    23641 ccgccatcgg gatcgcgctg agcgcgatga cgatcgccga gcggagcacc acggcgcggt
    23701 cgacgttcgg gctgtaccgc ctggagatct ttgcggccgc cgtgaacgcg gtcatcctgc
    23761 tcgccgttgc cggatgggtg gtgtggtcag cggtacgccg gctggaggat ccgccggagg
    23821 tggaaagcgg actgatgatc ctcgtcgcgc tcatcggact gctcgccaac gcgttctcgc
    23881 tgtggtggct gcgggaaggg caggcgtcga acctcaacgt gcgtggcgcc tacctcgagg
    23941 tgctcggcga cct
//
LOCUS       DATBFZ010000583         5997 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_5885938, whole genome shotgun sequence.
ACCESSION   DATBFZ010000583 DATBFZ010000000
VERSION     DATBFZ010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 5997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5997
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5885938"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(127..1002)
                     /locus_tag="VH419_17885"
     CDS             complement(127..1002)
                     /locus_tag="VH419_17885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7045547.1"
                     /translation="MGLTGVIYALVVLAWAAYLLPQALRRHDEAARKHSIERFSSAMR
                     VLARRGRSEHGRLVVTPPRSVDRVLTPSLTSPASAGTAREQRPRPSRAALKVAATRRR
                     RVLVVLVALAVLIAVAAVVGLIPWWSTAVPVALIVAFLLLARRQVRRATDAYWDEVAD
                     AHVEPTNVITRRATRVEASHGATRPADSDDEPTVTLDPDSLREARVDVGVLETADGGS
                     LWDPLPVTLPTYVDKPVARRSYRTVELAESGTWSSGHTSEPELDAPRPSSAAMRDEVS
                     EQDVEAGEAPQAVNG"
     gene            complement(1290..1922)
                     /locus_tag="VH419_17890"
     CDS             complement(1290..1922)
                     /locus_tag="VH419_17890"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEY7045548.1"
                     /translation="MPGRHVLTRGWPVVLRSGRIGLRPISRRDAARWAELRRANLDWL
                     GPWESTRPPDSSGHSSGYRALTRDLIKQGKQGRALPFVVTFDDVMVGQLTVSSITWGS
                     ARWAQMGYWIDEGHAGRGIMPTAVAMACDHCFSALGLHRIEIAIRPENVASLRVVEKL
                     GFTQIGIAPRYLHINGSWRDHLLFALTVEEVPEGVAARLRAHNSTDSHNP"
     gene            complement(1901..2413)
                     /locus_tag="VH419_17895"
     CDS             complement(1901..2413)
                     /locus_tag="VH419_17895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020392710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor synthesis domain-containing
                     protein"
                     /protein_id="HEY7045549.1"
                     /translation="MTDLADRRAHVVSVSNRASAGVYEDTTGPRIVAALREAGFGEVT
                     SSVVPDGEPVAEVLQGAVDEGVDLVVTTGGTGLTPTDRTPEMTRSVLDRDVPGVAEAI
                     RAYGRDNGIATAALSRGLVGVAGTTFVVNLPGSPGGVKDGLAVLLPILGHALDQLAGG
                     DHERSRGGTS"
     gene            complement(2410..2937)
                     /gene="moaC"
                     /locus_tag="VH419_17900"
     CDS             complement(2410..2937)
                     /gene="moaC"
                     /locus_tag="VH419_17900"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006131163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HEY7045550.1"
                     /translation="MPATPSDPEPRLTHVDQSGAARMVDVGAKAVSARTAVAAGRVLI
                     SSEVVALLRGDGVPKGDALGVARIAGIMAAKRTPDLVPLCHPIALSGVTVDLSVLDDA
                     VEITATVTTADRTGVEMEALTAVSVAGLALVDMVKAVDKAAVITDVRLLRKEGGRSGT
                     WTREQMRGGVEAELP"
     gene            complement(2937..4157)
                     /gene="glp"
                     /locus_tag="VH419_17905"
     CDS             complement(2937..4157)
                     /gene="glp"
                     /locus_tag="VH419_17905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007076872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HEY7045551.1"
                     /translation="MRSVEDHLNKILELVQPLPPYEQPLLEAIGLPICQDVTASIDLP
                     TFDNSAMDGYACRRDDLLDATEADPVRLPVVGESAAGQSKAYALSPGQAVKIMTGAPL
                     PTGADVVVPIEWTDGGRANVSITKLPTPGEHVRPAGEDVKAGQQVIEEGTVLGPRQAA
                     ILAATGHAQVRARPRPRIVVLSTGSELREPGTSLGFDSVYDANSFAIAAAARQADAIA
                     YRVGIVGDDPQELTDTLSDQLVRADLVVTSGGVSKGDYDVVKDVLSRLGTVQFTEVAM
                     QPGKPQGFGTIGEDDTPIFTLPGNPVSSYVSFEVFVLPAMRRMMGRLPYRRPLVRAEL
                     TDDISSPRDRRQFARGVFEVTTRGARVAPVGGHGSHLVGSLAQSNALIVVPEATTSID
                     AGTTVDVLVLDRSF"
     gene            complement(4245..5630)
                     /locus_tag="VH419_17910"
     CDS             complement(4245..5630)
                     /locus_tag="VH419_17910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015583191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HEY7045552.1"
                     /translation="MSAEGRAAAQQKMRDRGVHQAAIDVFLHYYEQLESGATGLIHES
                     DIRPLEDPPRLAHLPGDEAKDRAALAATTVIKLNGGLGTSMGMAGPKSLLEVREGLTF
                     LDIIARQVLAARQRWDVSLPVLFMNSFRTRDETLEALAAYDDLAVEGLPMDFMQHREP
                     KLLADDLTPVDWPREPELEWCPPGHGDLYTALSASGLLQLLRDAGYRYAFVSNSDNLR
                     ATVDARVAGWFASSGAPFASEITLRTPADRKGGHLAVRVDDGRLVLRETAQTSPEDAD
                     ALADISRHRYCNTNNLWLDLDALATKLEETQGFLGLPLIKNEKTVDPGDRSSPKVIQI
                     ESAMGAAVEVFEGSQALEVERTRFLPVKSTNDLLGLRSDVYQLADGCELRYADGVDEP
                     PLVDLDDDFYKIVGDFDKRFPHGPPSLRDARSFTVEGDWTFGPDVVVRGDVTVDTEGS
                     PGTISATTLSG"
     gene            5649..>5997
                     /locus_tag="VH419_17915"
     CDS             5649..>5997
                     /locus_tag="VH419_17915"
                     /inference="COORDINATES: protein motif:HMM:NF013934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEY7045553.1"
                     /translation="MGQVLQTKHELRALHATRRQARGESDQERLALGFRDQLLGVPEV
                     SAARRVAAFVSQPGEPGTGPLLETLHAHGVDVLLPFLRADFDLDWGVYRPGELQVGRF
                     GLLVPSTPLEGVGS"
BASE COUNT          925 a         2095 c         2047 g          930 t
ORIGIN      
        1 gaacgagacg cgctaccaac tgcgccacag ccccgggatc ggtcggcctt ccgaaccgcg
       61 ggttcgggcg gccgatcgcc gggaaactct agcaccaggc gtgggtgcgg ccgaaaacga
      121 gaacggtcag ccgttgaccg cttggggagc ctcaccggcc tccacgtctt gctcgctgac
      181 ctcatcacgc atggcggcgg acgagggccg aggcgcgtcc agttcgggtt ccgatgtgtg
      241 gccggacgac caggtgcccg actcggcgag ctcgacggtg cggtagctcc gccgcgccac
      301 tggcttgtcg acgtacgtcg gcagggtcac cggcagcggg tcccacagcg acccgccgtc
      361 cgcggtctcg aggactccca cgtcgacgcg tgcttcgcgc aggctgtcgg gatccagcgt
      421 gaccgtcggc tcgtcgtcgg agtccgccgg ccgagtcgcg ccgtgcgagg cctcgaccct
      481 ggtcgcccgg cgcgtgatga cgttggtcgg ttcgacgtgt gcgtcagcca cctcgtccca
      541 gtacgcgtcg gtcgctcggc gtacctggcg ccgggccagc agcaggaacg cgacgatcaa
      601 cgcgaccggc accgccgtcg accaccaagg gatcaggccg accactgcgg cgacggcgat
      661 gaggacggcg agggcgacga gcacgacgag cacgcgacga cgtcgggtcg ccgcaacttt
      721 caaggctgcc cggctgggtc gcggtcgttg ttcgcgcgcg gtcccggcac tcgccggcga
      781 ggtcagggac ggcgtcagca cccggtccac gcttcggggt ggtgtcacga ccaggcgacc
      841 gtgttccgac cggccgcgcc gcgccaggac gcgcatggcc gaggagaacc gttcgatgga
      901 gtgcttgcgg gccgcttcgt cgtgtcgacg caacgcctgc ggaaggaggt aggccgccca
      961 ggccaggacg acaagcgcgt agatgactcc ggtcaaaccc acggttacga ccgtatggcc
     1021 ggaattcgaa ccggcagcgg agcgaggccg gtgtgtcgtc tgagaattgt gtgacggaag
     1081 tgaatcgagt gttcaccgga tctgctgcgc ccgacgaggg cggccgagca tttgcgatgt
     1141 ccacatccga gatccactgt gcccatcgga ggtctgcgat gtcgacacgc cgcttgggct
     1201 gtaaacatcg caaaccacca actccacgcc aagcccgtgc gtttggatgg ctaccgaggc
     1261 aaaccgtcac cgacggacag ggtcagacgt caggggttgt gggagtcggt cgagttgtgg
     1321 gcgcgaagac gggcggcgac tccctcgggg acctcttcga cggtcagcgc gaacaacagg
     1381 tggtcgcgcc agcttccgtt gatgtgcaga tagcgcggcg cgatcccgat ctgggtgaag
     1441 ccgagcttct cgaccactcg cagactggcc acgttctcgg ggcggatcgc gatctcgatc
     1501 cggtgtagcc cgagcgccga gaagcagtgg tcgcaggcca tggccaccgc cgtcggcatg
     1561 atgcctcggc ccgcgtgccc ttcgtcgatc cagtagccca tctgcgccca ccgcgccgag
     1621 ccccaggtga tgctcgacac cgtcagctgg ccaaccatca cgtcgtcaaa ggtgacgacg
     1681 aacggcagcg cacgaccctg cttcccctgt ttgatcaggt cacgtgtgag ggcgcggtag
     1741 ccggaggaat gcccgctgga gtcgggcggg cgggtcgact cccacggacc caaccagtcg
     1801 aggttcgcgc gacgcagctc ggcccagcgg gcggcgtcac ggcgcgagat cggccgcagt
     1861 ccgatccgcc cgctccgtaa gacgacgggc cagccgcgag tcaggacgtg cctccccggg
     1921 atcgttcgtg gtcgccgccg gccagctggt cgagcgcgtg gcccaggatc gggagcagca
     1981 cggcaagacc gtccttcacg cctcccggcg agccggggag gttgacgacg aacgtcgtac
     2041 cggccacacc gacgagacca cgtgacagcg ctgcggtggc gatcccgttg tcccggccgt
     2101 acgcgcggat cgcctccgcc acaccaggta cgtcgcggtc gagcaccgag cgggtcatct
     2161 ccggcgtccg gtcggtcggc gtgagaccgg tgccgcctgt cgtgacgacc aggtcgacgc
     2221 cttcatcgac cgcaccctgc agcacctcgg cgacaggttc gccatcgggt acgacgctgg
     2281 atgtcacctc gccgaatccc gcctcgcgca aggcagccac gatgcgcggg ccggtcgtgt
     2341 cctcgtagac acccgccgat gcccggttgg acaccgagac gacatgcgct cgccggtccg
     2401 ccaggtcggt cacggcaact ccgcctcgac accgccacgc atctgctccc gcgtccacgt
     2461 acccgaccgg ccgccctcct tgcggagcag ccggacgtcg gtgatgacgg cggccttgtc
     2521 gaccgctttc accatgtcga ccaacgccag tccggcgacg ctcactgccg tcaacgcctc
     2581 catctcgact ccggtacggt ccgcggtcgt cacggtcgcg gtgatctcga ccgcgtcgtc
     2641 gaggaccgaa aggtcgaccg tgacgccgct caacgcgatc ggatggcaca gcggcacgag
     2701 gtcgggggtg cgtttggcgg ccatgatgcc tgcgatcctg gcgactccca gcgcgtcccc
     2761 cttcggcacg ccgtcaccgc gcagcagcgc caccacctcc gacgagatca gcacgcgtcc
     2821 ggcggcgacg gccgtccgcg cgctgaccgc cttcgctccc acgtcgacca tgcgtgccgc
     2881 ccccgactgg tcgacgtgcg tcaaccgggg ctctgggtcg gacggcgtgg caggcatcag
     2941 aagctccggt ccagcaccaa cacgtcgacc gtcgtacccg cgtcgatcga ggtcgtcgcc
     3001 tcaggcacca cgatcagcgc gttcgactgc gcgaggctgc cgaccaggtg cgagccatga
     3061 ccgcccaccg gcgcgacccg cgcccctcgc gtcgtcacct cgaagacgcc tcgggcgaac
     3121 tgcctgcggt ccctcggcga gctgatgtcg tcggtgagct cggcgcggac gagtggtctg
     3181 cggtacggca gccggcccat catccggcgc atcgccggca gcacgaacac ctcgaaggag
     3241 acgtacgacg acacgggatt gccgggcagc gtgaagatgg gggtgtcgtc ctcgccgatc
     3301 gtcccgaagc cctgcggctt gcccggctgc atggccacct cggtgaactg gaccgtcccg
     3361 agccggctga gcacgtcctt gacgacgtcg tagtccccct tgctcacgcc gccgctcgtc
     3421 accaccaggt ccgcccggac gagttggtcg gacagcgtgt cggtgagctc ctgcgggtcg
     3481 tcgccgacga tgccgacgcg gtaggcgatc gcgtcggcct gccgagccgc cgcggcgatc
     3541 gcgaacgagt tcgcgtcgta gaccgagtcg aacccgagcg acgtacccgg ctcgcgcaac
     3601 tcgctaccgg tcgacagcac gacgatgcgc gggcgcggcc gagcccgcac ctgggcgtgc
     3661 ccggtcgcgg ccaggatcgc ggcctgtcgc gggccgagca ccgtcccttc ctcgatgacc
     3721 tgctggcccg ccttcacgtc ctcgccggcg gggcggacgt gctcgccggg ggtgggcagc
     3781 ttcgtgatcg acacgttcgc acgaccgccg tccgtccact cgatcggtac gacgacgtcg
     3841 gcaccggtgg gcagtggcgc ccccgtcatg atcttcacgg cctggccggg tgagagcgca
     3901 tacgccttgc tctgcccagc agcagactcc cccaccacgg gcagccggac agggtcggcc
     3961 tcggtggcgt cgagcagatc gtcacgccga cacgcgtagc cgtccatcgc cgagttgtcg
     4021 aacgtcggca ggtcgatcga ggccgtcacg tcctggcaga tgggcaggcc gatcgcctcg
     4081 agcagtggct gctcgtacgg cgggaggggc tggacaagtt ccaggatctt gttcagatgg
     4141 tcttcgacac tgcgcatcag gcaacgagtc tctcgcgtcg gggatcgtga ccacggcctc
     4201 ggggcctcaa cactagcctc gatgtttcgt ctgatggcta gacctcagcc ggagagcgtg
     4261 gtggcggaga tcgtgccggg tgacccctcc gtgtcgacgg tgacgtcgcc gcggacgacg
     4321 acgtcaggac cgaacgtcca gtcaccttcg acggtgaacg accttgcgtc acgcaacgac
     4381 ggcgggccgt gagggaaccg cttgtcgaag tcgccgacga tcttgtagaa gtcgtcgtcg
     4441 aggtcgacca ggggcggttc gtcgacgccg tcggcgtacc gcagctcgca cccgtccgcc
     4501 aattggtaga cgtccgaccg cagcccgagc aggtcgttcg tcgacttgac gggcaggaag
     4561 cgcgtccgct ccacctccag ggcctgtgag ccttcgaaga cctcgacggc cgcgcccatc
     4621 gccgactcga tctggatcac cttcggcgac gacctgtccc ccgggtcgac ggtcttctcg
     4681 ttcttgatga gcggcaggcc caggaaccct tgggtctctt ccagcttcgt ggccaacgcg
     4741 tccagatcca gccacaagtt gttcgtgttg cagtaacggt ggcggctgat gtcggccagc
     4801 gcatcggcgt cctccggaga cgtctgagcg gtctcgcgca ggaccagtcg tccgtcgtcg
     4861 acccggaccg ccaggtgtcc gcccttgcgg tcggccggcg tacgcagggt gatctcgctg
     4921 gcgaatggcg caccgctgga ggcgaaccac ccggcaaccc gggcatccac ggtggcgcgc
     4981 aggttgtcgg agttcgagac gaacgcgtac cggtagccgg cgtcgcgcag cagctgcagc
     5041 aggccggagg cggacaacgc cgtgtagagg tcgccgtgcc ctggcgggca ccactcgagc
     5101 tccggttcgc gcggccagtc caccggcgtc aggtcgtcgg cgagcagctt cggttcgcgg
     5161 tgctgcatga agtccatggg caagccctcg acggccaggt cgtcgtaggc cgccagcgcc
     5221 tcaagggtct cgtcacgggt ccggaacgag ttcatgaaca gcaccggcaa cgacacgtcc
     5281 catcgttggc gcgcagccag gacctggcgg gcgatgatgt ccaggaacgt caagccctca
     5341 cgcacctcga gcagcgactt cggcccggcc atgcccatcg acgtaccgag gccaccgttg
     5401 agcttgatca ccgtcgtggc ggcgagcgcc gcacggtcct tcgcttcgtc gccgggaagg
     5461 tgggcaagtc gaggcgggtc ctccaacggc cggatgtctg actcgtggat gaggccggtg
     5521 gcgccggact ccagctgctc gtagtagtgc aggaaaacgt cgatggcggc ctggtgcaca
     5581 ccgcggtcgc gcatcttctg ctgggcggcg gcacgtccct cggcgctcat accgggcatg
     5641 ctaacggcgt gggtcaggtt cttcagacca agcatgagct gcgggcgctc cacgcgactc
     5701 gtcgtcaggc acgaggcgag tcagaccagg aacggctcgc cctcggcttc cgcgaccagc
     5761 tgctcggcgt gccggaggtc agcgcggccc gacgggtcgc cgcgttcgtc tcgcagcccg
     5821 gtgagcccgg caccgggccg ctgctcgaga cactgcacgc ccacggcgtc gacgtactgc
     5881 ttccctttct gcgtgccgac ttcgacctcg actggggcgt gtaccggcct ggcgagctcc
     5941 aggtcggtcg gttcggtctc ctggtcccgt cgaccccgct cgaaggggtg ggctcca
//
LOCUS       DATBFZ010000584         2608 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_5893577, whole genome shotgun sequence.
ACCESSION   DATBFZ010000584 DATBFZ010000000
VERSION     DATBFZ010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5893577"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="VH419_17920"
     CDS             <1..257
                     /locus_tag="VH419_17920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012918020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY7045554.1"
                     /translation="ADVVDKHDEQDRAETRHVLLAALSELTRRQRATVVLRYYFDLSE
                     AETAAALGCSEGTVKSQAARGLARLRKSDLAELVHEGTSR"
     gene            254..1081
                     /locus_tag="VH419_17925"
     CDS             254..1081
                     /locus_tag="VH419_17925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7045555.1"
                     /translation="MTQLTEHQLSTLLKETAPEPSHDTVRAAEARRLAGRMRRRRWMT
                     SGVLVAVIAVASPVAVVVGQSSTDSQPASSVPTKPEHSPVSALNSDCLNGATCDPASV
                     YAAIDRPLHLPSLAPGQACPVSPTRTFTGGAGFTGRFTGIGSGPLYLTPFGRKPSDAL
                     LLTAGYGSRQWPGYKVIWVIDRYYGGPLLLRGGRIDKPGELRFERYLGAADYPKSSIP
                     ADRPQHSVLYVRGGLGAEGPRVLDSSPSGVFAQSSGCYAIQVDGQGFTETIVFRAAL"
     gene            complement(1259..>2608)
                     /locus_tag="VH419_17930"
     CDS             complement(1259..>2608)
                     /locus_tag="VH419_17930"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015070162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEY7045556.1"
                     /translation="ERTVQSLVGATQANVHLYNATAPLFRRVVFAVDRDECRAIATRG
                     TEIVMKYADELLGGTDFGYQYSPEIFTGTELEFAVDVCESVSDVWEPGPGREIILNLP
                     ATVEMATPNVYADQIEWFSRHLTRREHSVVSLHPHNDRGTAVAATELALMAGADRVEG
                     CLFGHGERTGNVCLVTLGMNLFSQGIDPMIDFGDIDEIRRTVEYCTQLPVHPRHPYAG
                     DLVYTAFSGSHQDAIKKGLEALEADASQGVGDLTHPWAVPYLPIDPKDVGRSYEAVIR
                     VNSQSGKGGVAYIMKAEHKLELPRRLQIEFSRVVQGLTDEEGGEISADEIWSAFRTEY
                     LERRTPLALNSVHTSSAAGEHDALTVNVYVDGENRTLQGSGNGPIAAFVTALDDIGYD
                     VRVLDYAEHALSAGGDATAAAYVECAVGDQVLWGVGLDSNIVTASLKAVISAINRAA"
BASE COUNT          419 a          889 c          869 g          431 t
ORIGIN      
        1 tggccgatgt cgttgacaag cacgacgagc aggaccgtgc cgagacgcga cacgtgctgc
       61 tggctgcgct gtccgagctg acgcgcagac aacgggccac ggtggtcctt cgctactact
      121 tcgacctctc ggaggcggag accgcggccg cgctcggctg ctccgaggga accgtcaaga
      181 gccaggcggc caggggcctt gcccggctcc gcaagtccga cctggcagaa ctggtccacg
      241 aagggacgag ccgatgaccc agctgaccga gcaccagctg tcgaccctgt tgaaggaaac
      301 cgcgccggag cccagccacg acacggtccg cgccgccgag gcacgacgac ttgccggacg
      361 gatgcgtcga cggcgatgga tgacatcggg tgtcctggtc gccgtgatcg ccgtggcgag
      421 cccggtcgct gtggtcgtcg gacagtcatc caccgactcg caacccgcca gctccgtgcc
      481 gacgaagccc gagcactcgc ctgtctcagc gctcaacagc gactgcctga acggcgccac
      541 gtgcgaccca gcttccgtgt acgccgcgat cgacagaccg ctgcacctgc cctcgcttgc
      601 acctggacag gcctgcccgg tgagcccaac gcgcaccttc accggcgggg cgggtttcac
      661 cggcaggttc accggcattg gtagcggacc tctctacctg acgcccttcg gccggaagcc
      721 ttccgatgcg ctgttgttga ccgctggcta cggctcgagg cagtggcccg gctacaaggt
      781 gatctgggtg atcgaccgct actacggcgg gccactcctg ctgcgtggcg gacgcatcga
      841 caaaccgggc gagctccggt tcgagcgcta cctcggtgcg gcggactatc cgaagtcctc
      901 gatccccgca gaccgacctc agcattcagt gctctacgtc cgcgggggcc taggggctga
      961 gggaccgcgg gtcctggact cctccccgag cggagttttc gcgcaatcat cgggttgtta
     1021 cgccatccag gtcgacggcc agggcttcac cgaaaccatc gtcttccgcg ccgccctctg
     1081 atcgacccac gccgaacggc ccgctgatcg acctctcagc ccgccgatcg agcttgtcga
     1141 gatcaaggac cgccgcggac cgctgatcga gcttgtcgag accaaggacc gccgcggacc
     1201 gctgatcgag cttgtcgaga tcaagggctc gtcggttcgg tgccaccacc gcccgaagtc
     1261 aggcagccct gttgatggcg gagatgacgg ccttgagcga ggcggtgacg atgttcgagt
     1321 cgagcccgac accccacagc acctggtcgc cgacggcgca ctcgacgtag gcggcggcgg
     1381 tcgcgtcacc acctgccgac aacgcgtgct cggcgtagtc gaggactcgg acgtcgtacc
     1441 cgatgtcgtc gagcgcggtg acgaaggccg cgatcggacc gttgcccgaa ccttgcaggg
     1501 tgcggttctc gccgtcgacg tacacgttga cggtgagtgc gtcgtgctcg cctgccgccg
     1561 acgacgtgtg cacggagttc agcgcgagcg gcgtacgacg ctccaggtac tccgtgcgga
     1621 acgccgacca gatctcgtcg gccgagatct cgccaccctc ctcgtcagtc agcccctgga
     1681 cgacccggct gaactcgatc tgcagccggc gtggcagctc gagcttgtgc tcggccttca
     1741 tgatgtacgc gacgccgccc ttgcctgact gggagttgac gcgaatgacc gcctcgtagg
     1801 agcggccgac gtccttcggg tcgatgggca ggtacggaac cgcccatgga tgggtgaggt
     1861 cgccaacgcc ctggcttgcg tcggcctcca gggcttcgag acccttcttg atggcgtcct
     1921 gatgggagcc ggagaacgcc gtgtagacca ggtcgccggc gtacgggtgg cgaggatgga
     1981 ccggcagctg cgtgcagtac tcgaccgtac gccggatctc gtcgatgtcg ccgaagtcga
     2041 tcatggggtc gatcccctgg ctgaacaggt tcatgccgag cgtcaccagg cagacgttcc
     2101 ccgtgcgctc gccgtggccg aacaggcagc cctcgacgcg gtcagccccg gccatgagcg
     2161 cgagctcggt ggccgcgacc gctgtgccgc ggtcgttgtg cgggtgcagt gagacgacgg
     2221 agtgttcccg ccgcgtgagg tgccggctga accactcgat ctgatcggcg tacacgttcg
     2281 gcgtcgccat ctcgacggtg gcgggcaggt tgaggatgat ctcccgtcct ggtcccggct
     2341 cccacacgtc agacaccgac tcgcacacgt cgacggcgaa ctccagctcg gttccggtga
     2401 agatctcggg gctgtactgg tagccgaagt cggtgccgcc gaggagctcg tcggcgtact
     2461 tcatcacgat ctccgtgccg cgcgtggcaa tcgcccggca ctcgtccctg tcgacggcga
     2521 acacgacacg ccggaacagc ggagccgtcg cgttgtagag gtggacgttc gcctgagtgg
     2581 cgccgaccag gctctggacg gtgcgctc
//
LOCUS       DATBFZ010000585         2339 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_5895852, whole genome shotgun sequence.
ACCESSION   DATBFZ010000585 DATBFZ010000000
VERSION     DATBFZ010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5895852"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1673)
                     /gene="kdpB"
                     /locus_tag="VH419_17935"
     CDS             complement(<1..1673)
                     /gene="kdpB"
                     /locus_tag="VH419_17935"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012924336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HEY7045557.1"
                     /translation="MSKHQPSRVVSAGVLDPRLLSRSLPDALRKLDPRSMVHAPVMLV
                     VEIGSAVSTVLAVKHPTVFGWTIVVWLWLTVVFANLAEAVAEGRGKAQAAALRKTRQE
                     TVARRLTATGEETVPATALQLGDRVVVEAGGTIPGDGDVIEGVASVDESAITGESAPV
                     IRESGGDRSSVTGGTKVLSDRIVVSITSRPGDTFVDKMIALVEGASRQKTPNEVALNI
                     LLASLTIIFLAATVTLQPFAIYSGAPQSLIVLTALLVCLIPTTIGALLSAIGIAGMDR
                     LVQHNVLATSGRAVEAAGDVNTLLLDKTGTVTLGNRQASDFIAVAGTTAETLADAAQL
                     ASLADETPEGRSVVVLAKQRHGLRARTPGELAAVQEVPFSAQTRMSGVDLDTGRRIRK
                     GAASAVVRWVEEQGGTTPDEVRQVVDGISRSGGTPLVVAEKPADAAARVLGVIQLKDV
                     VKDGMRERFDEMRRMGIRTVMITGDNRLTAKAIADEAGVDDFLAEATPEDKMALIRKE
                     QAGGRLVAMTGDGTNDAPALAQADVGVAMNTGTQAAREAGNMVDLDSNPT"
     gene            complement(1716..>2339)
                     /locus_tag="VH419_17940"
     CDS             complement(1716..>2339)
                     /locus_tag="VH419_17940"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020577378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HEY7045558.1"
                     /translation="FHSSFSALGGGLLILNMGLGEVAPGGVGSGLYGMLVLAIVAVFV
                     AGLMVGRTPEYLAKKIQAREIKLISLSILTMPLLVLVGTGLAMAMSSARASILNPGPH
                     GLSEVLYGFMSASNNNGSAFAGLAADTTFYNTALGLCMLIGRFLPIMFVLALAGSLAV
                     QKPVPATAGTLPTHRPLFVGLLVGVALIVAGLTFFPALALGPLAEGL"
BASE COUNT          378 a          794 c          804 g          363 t
ORIGIN      
        1 gtcgggttgg agtcgaggtc gaccatgttg cccgcctcgc gcgccgcctg cgtgccggtg
       61 ttcatcgcca ccccgacgtc ggcctgcgcc aaggcagggg cgtcgttggt gccgtcaccg
      121 gtcatcgcga ccagccggcc gcccgcctgc tccttgcgga tcagggccat cttgtcctcg
      181 ggcgtcgcct ccgcgaggaa gtcgtcgacg cctgcctcgt ccgcgatggc cttggcggtg
      241 agccggttgt cgcccgtgat catcaccgtg cggattccca tccggcgcat ctcgtcgaac
      301 cgctcgcgca tgccgtcctt gacgacgtcc ttgagctgga tgacaccgag aactcgcgcg
      361 gcggcgtcgg caggcttctc cgcgacaacc agcggcgttc cgccggacct ggatatcccg
      421 tcgaccacct ggcgcacctc gtccggtgtg gtaccaccct gctcctcgac ccagcgaacg
      481 acggcggacg ccgcgccttt gcggatcctc cggccggtgt cgaggtcgac cccgctcatc
      541 ctggtctgcg cgctgaacgg aacctcctgg acagcggcga gctcccccgg agtacgagca
      601 cgcagcccgt gccgttgctt ggcgagtacg acgaccgagc gtccctccgg cgtctcgtcc
      661 gccaggctgg cgagctgggc cgcatcggca agtgtctcgg ccgtggtgcc cgcgacggcg
      721 atgaagtcgc tggcctgtcg gttgccgagc gtcaccgtgc cggtcttgtc gagcagcaac
      781 gtgttgacgt ctccggctgc ctccaccgca cgtccggatg tggcgagcac gttgtgctgc
      841 acgaggcggt ccatcccggc gatgccgatg gccgacagaa gggcgccgat ggtggtcggg
      901 atcaggcaca cgagcagcgc cgtcaggacg atgagcgact gaggcgcgcc ggagtagatc
      961 gcgaagggct gcagcgtcac ggtcgcggcc aggaagatga tggtcaggct tgccagcagg
     1021 atgttgagcg cgacctcgtt cggggtcttc tggcgcgacg ctccttcgac gagcgcgatc
     1081 atcttgtcga cgaacgtgtc gcccggccgc gacgtgatcg agacgacgat ccggtccgac
     1141 aagaccttgg tgccaccggt caccgagctg cggtctccgc cggactcgcg gatgacggga
     1201 gccgactcgc cggtgatggc ggactcgtcg acgctcgcca caccctcgat gacgtcaccg
     1261 tcacccggga tcgtcccgcc ggcctcgacg acgacacggt cgccgagctg gagcgcggtc
     1321 gcagggacgg tctcctcgcc ggtcgctgtg agacgccggg cgacggtctc ctgccgggtc
     1381 ttgcggagcg ccgcagcctg cgccttcccg cgcccctcgg cgaccgcctc cgcgagattc
     1441 gcgaacacga ccgtcagcca gagccagacc acgatggtcc agccgaagac ggtcgggtgc
     1501 ttgacggcga gcaccgtgga gacagccgat ccgatctcga cgacgagcat cacgggcgcg
     1561 tgcaccatcg accgcgggtc gagcttgcgg agggcgtcgg ggagcgacct gctcaataac
     1621 ctcggatcga ggactcccgc tgacacgacg cggctcggtt gatgcttcga catcccgtcg
     1681 acggagggtt cgtggcgctc gagaacggtc atggctcaca ggccttctgc gagcgggccg
     1741 agggcgagtg cggggaagaa cgtcaacccc gcgacgatga gtgccacgcc gaccaggagt
     1801 ccgacgaaca acggacgatg tgtcgggagc gttccggcgg tggcggggac gggtttctgc
     1861 accgccagcg aaccggccag tgcgaggacg aacatgatgg gcaggaatct cccgatcaac
     1921 atgcacagac ccagagccgt gttgtagaag gtcgtgtcgg cggccagccc ggcaaacgcg
     1981 gagccgttgt tgttgctcgc cgacatgaag ccgtacagca cctccgacag gccgtgcggt
     2041 ccggggttga ggatggacgc gcgggcgctc gacatcgcca tcgcaagtcc ggtcccgacg
     2101 agcacgagca gcggcatggt gaggatcgag agtgagatga gcttgatctc gcgagcctgg
     2161 atcttcttcg cgaggtactc cggcgtacgt ccgaccatca ggccggcgac gaacaccgcg
     2221 acgatcgcga gcaccaacat gccgtagaga cccgagccga cgccgccagg cgccacctca
     2281 ccgaggccca tgttgaggat caagagcccg ccgcccagcg cgctgaacga cgagtggaa
//
LOCUS       DATBFZ010000586         2356 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_6557602, whole genome shotgun sequence.
ACCESSION   DATBFZ010000586 DATBFZ010000000
VERSION     DATBFZ010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6557602"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..620)
                     /locus_tag="VH419_17945"
     CDS             complement(<1..620)
                     /locus_tag="VH419_17945"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEY7045559.1"
                     /translation="MEETTENPIGFDSARVTTWLAERVPGLQPPLQWTKLPGGHSNFT
                     YRVDDTAGRTLVVRRPPLGELLPTAHDMRREFTVISALWPTPVPVPEPIAYTDDEAVT
                     GAQFYVMGWVEGRSLYTAVETAAHIPSEEARARTGPSFIDVLAALHSLDPDDVGLGAL
                     GKRTSYVGRQLHRWYESWNASKNRELPDVDRLYTFLVDRLPEQTKTT"
     gene            complement(713..1984)
                     /locus_tag="VH419_17950"
     CDS             complement(713..1984)
                     /locus_tag="VH419_17950"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY7045560.1"
                     /translation="MDFELPEELQALLDEIDDFIEREIKPLEEEDDNIRFFDHRREDS
                     RTDWDRGGLPNDEWENLLRRARQRADDAGLYRYAFPKEYGGRDGTNLGMAVIREHLAR
                     KGLGLHCDLQNEHAVVGNNVGLLLMLAYGTDKQKKEWVDDLAQGRRGFAFGITEPNHG
                     SDATFMETHAERDGDEWIINGEKTWNTGIHSAQYDMIMARTSGKPGDGDGITAFLTPT
                     DSPGFKVEEFLWTFNMPTDHARVSFTDVRVPHENIFGGEGKGLAVVQLFFNENRIRQA
                     ASSLGAAEYCIDESVKYAKERKPFGKPLSVNQAIQFPLVELQTQCEMLRALIQKTAWM
                     MDKYGAFSQSDKVSMCNYWGNRLCCEAADRAMQVHGGMGYSRHKPFEHIYRHHRRYRI
                     TEGSEEIQMRRVAGYMFGFMHQQAPKGVAEG"
     gene            complement(2062..2253)
                     /locus_tag="VH419_17955"
     CDS             complement(2062..2253)
                     /locus_tag="VH419_17955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY7045561.1"
                     /translation="MPKRWQSLSNEELREVHLALALRITQLSTMDTSKMGPSAKAKMT
                     RHLDLCTELNDTVQKARGK"
BASE COUNT          358 a          755 c          772 g          471 t
ORIGIN      
        1 gtggtcttcg tctgctcggg gaggcgatcg acgaggaacg tgtagagacg gtcgacgtcg
       61 ggcagctccc gattcttcga cgcgttccac gactcgtacc accgatgcaa ctggcgcccc
      121 acgtaggagg tgcgcttgcc tagcgcaccc aggccgacgt catcgggatc gaggctgtgg
      181 agtgcggcga gaacgtcgat gaacgacggg ccggtgcgag cacgcgcctc ctcgctcgga
      241 atgtgcgccg ccgtctcgac agcggtgtag agcgagcggc cctcgaccca tcccatgacg
      301 tagaactggg cccccgtcac ggcttcgtcg tcggtatacg cgatgggttc ggggacgggc
      361 acgggcgtgg gccacagcgc cgagatgacg gtgaactcgc ggcgcatgtc gtgggccgtc
      421 ggaagcagct cgccgagcgg cgggcggcgc acgaccaacg tccgccccgc cgtgtcgtcc
      481 acgcgatagg tgaagttgga gtggccaccc gggagcttgg tccactgcaa cggcggctgc
      541 agcccgggga cgcgctcggc gagccaggtc gtcacccgcg cgctgtcgaa gccgatcggg
      601 ttctcggtcg tctcctccat gcgcgcggat gctacggcag aacactgttg ggtcactttt
      661 tggcgccaga gtgctcatcc gcgacccaac agtgaacgcc ggtcttcgcg cctcagcctt
      721 cggccacacc cttgggcgct tgttggtgca tgaagccgaa catgtagccg gcgacgcgcc
      781 gcatctggat ctcttcgctt ccttcggtta tgcggtagcg ccggtgatgg cggtagatgt
      841 gctcaaacgg cttgtgacgg gaatagccca tgccgccgtg gacctgcatg gctcggtcgg
      901 ccgcctcgca gcacaagcgg ttcccccagt aattgcacat cgagaccttg tcggactgcg
      961 agaaagcccc gtacttgtcc atcatccacg ccgtcttctg gatgagggcg cggagcatct
     1021 cgcattgggt ctggagttcc acgaggggga actggatcgc ctggttgacg gagagcggct
     1081 tgccgaacgg cttgcgttcc ttggcgtatt tcaccgactc gtcgatgcag tactcggcgg
     1141 cgccgaggct ggaggccgcc tggcggatgc ggttctcgtt gaagaagagt tgcacgaccg
     1201 ccagaccctt gccctcacct ccgaagatgt tctcgtgcgg cacgcgcacg tccgtgaacg
     1261 acacgcgggc gtggtccgtc ggcatgttga acgtccacag gaactcttcg accttgaatc
     1321 caggcgagtc cgtcggcgtc aggaacgcgg tgatgccgtc gccgtcgccg ggcttgccgc
     1381 tcgtccgcgc catgatcatg tcgtactgag cactgtggat accggtgttc caggtcttct
     1441 caccgttgat gatccactcg tcgccgtcgc gctcggcgtg ggtctccatg aaggtggcat
     1501 cggagccgtg gttcggctcg gtgatgccga acgcgaagcc ccgccgcccc tgggcgaggt
     1561 cgtcgaccca ctccttcttc tgcttgtcgg tgccatacgc cagcatgagg agcaggccga
     1621 cgttgttgcc gacgacggcg tgctcgttct ggaggtcgca gtgcagtccg agccctttgc
     1681 gggcgaggtg ctcacggatg accgccatgc cgaggttggt gccgtccctg ccgccgtact
     1741 ccttcgggaa cgcgtagcga tacaggccgg catcatcggc ccgctgccgg gccctccgta
     1801 gcaggttctc ccactcgtcg ttcggcagcc caccccggtc ccagtcggtg cgagagtcct
     1861 cccggcggtg gtcgaagaag cggatgttgt cgtcctcctc ctcgagaggc ttgatctccc
     1921 gctcgatgaa gtcgtcgatc tcgtccagca gcgcttggag ttcctcgggg agttcgaagt
     1981 ccatgttctc gatcctccta tgtccgtgac cgcagatcga aactcggcgg acgtctagcg
     2041 tcaaatccgg acggcgccgg cctacttgcc gcgtgccttc tgcacggtgt cgttgagttc
     2101 agtgcagagg tcgaggtggc gcgtcatctt cgccttcgcc gacgggccca tcttgctggt
     2161 gtccattgtg ctcaactgcg tgatgcgcag tgccaaggcg agatgaacct ctcgcagctc
     2221 ctcgttcgag aggctttgcc agcgcttggg cacacgcctt cgttagcaca gcggcagttc
     2281 caattgcacc gctgcgtttt ctgccgcttg acgcagcgcg acgccagtgt cgtacgtgcg
     2341 tgacactgtg cgccat
//
LOCUS       DATBFZ010000587         6978 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nocardioidaceae bacterium isolate SMAG_U3824
            k141_5900765, whole genome shotgun sequence.
ACCESSION   DATBFZ010000587 DATBFZ010000000
VERSION     DATBFZ010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958730
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nocardioidaceae bacterium (soil metagenome)
  ORGANISM  Nocardioidaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae.
REFERENCE   1  (bases 1 to 6978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 08:25:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,597
            CDSs (total)                      :: 3,554
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6978
                     /organism="Nocardioidaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5900765"
                     /isolate="SMAG_U3824"
                     /isolation_source="soil"
                     /db_xref="taxon:1871072"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1338)
                     /locus_tag="VH419_17960"
     CDS             complement(<1..1338)
                     /locus_tag="VH419_17960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008359728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 2,3-aminomutase"
                     /protein_id="HEY7045562.1"
                     /translation="MTDLTGLDRDETPFLAEGLEQPYPYKRVELVEPDWTRFPGWRDV
                     TAEDWASVQWQRAHCVKNIKQLRQVMGDLIEDRFYDDLQRDQQERATMSMLMPPQMVN
                     TMVPHATPTTDLLYADPVRRYMLPVFSDRRTDWPSHPHATRDSLHEHDMWVAEGLTHR
                     YPTKVLAEVLPTCPQYCGHCTRMDLVGNSTPVIDKLKFHLKPVDRLDAMIEYLRSTPG
                     VRDVVVSGGDVANMPWPRLEGFVDQVLDVDNIRDIRLATKALMGLPQHWLQDDVRAGM
                     ERLATKAREREASIAIHTHVNHANSVTPMVAKATRAMLDTGIRDVRNQGVLMGGVNDD
                     AHTLLDLCFALLDGAQIMPYYFYMCDMIPFSEHWRVSVARAQELQHGIMGYLPGFATP
                     RIVCDVPFVGKRWVHQLESYDREHGISYWTKNYRTSIERTDPDALTRTYEYYDP"
     gene            1472..2530
                     /locus_tag="VH419_17965"
     CDS             1472..2530
                     /locus_tag="VH419_17965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-erythro-3,5-diaminohexanoate dehydrogenase"
                     /protein_id="HEY7045563.1"
                     /translation="MNVASDPIGLHRVVAPAGVLPQAAQRLDTSPEIGPDEIRIDVTR
                     LNLDAASFRQLFDKHGGDGAAVRAEVLEIISTRGKMHNPVTGSGGMLTGTVAAVGPES
                     SVGLSVGDEVTTLVSLTLTPLVITDRLANWDGRSEQVPCEGYAILFGRTIVAKLPTDL
                     PARLALAVMDVCGAPALTARVVGGYAARGSAPTVCVIGGAGKSGSLSLAAAKDAGAGR
                     TIAVVPVQGEVDLLADKQLVDVVTLADARDPVALSQAVLDAGGPADVTVVCVDVPGCE
                     GGAILSTAQGGTVVFFSMATSFTAAALGAEGLAADVTMLIGNGYLPGHADYAVDLLRR
                     HSGVRALFESRLAHEAAG"
     gene            2536..4170
                     /locus_tag="VH419_17970"
     CDS             2536..4170
                     /locus_tag="VH419_17970"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012921216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEY7045564.1"
                     /translation="MTASSESAMADHVDVPTGGNPVPRRVLLRGGHICSPVSPSATAM
                     FTVNGAVAWVGDDAAASSYADTADEVVQLDGRLVTPGFVDAHVHLAQTGFALASLDLS
                     TTRTAAEALDLVAAFARRSDGAVLFAYGWDESRWADRRPFTAGQLDRAAPGRVLYAAR
                     VDSHSAVVSSRLIEHDPSIRTRDGWRGDGWVARDAHHAARAVTDALRSPSDREQALLA
                     ALTHAASRGITSVHELNAPHIAPVSDFAALRTLAEHTPLPEVVPYWGELLGEGLDDAH
                     VTGLSGFAGDLCVDGAIGSRTACMHAPYADADTSGHLYLDRDEIAEHVVFCTDRDVQA
                     GFHVIGDRAMSETVAGLELAADKVGTDKIVAARHRLEHVEMPTPEGVATLARLGVVAS
                     VQPAFDAAWGAAGELYEQRLGADRARPMNPFGSMRRAGIVLAFGSDSPITPIDPWSAV
                     RASVLHHDEDERLSPQAAFEAHTRGGHAARRDDTGGVLEPGASASYVVWDLSDGAVQT
                     PSGGSALPDLDPHLPLPTCVQTVVAGVSVYSAEDSA"
     gene            4167..5765
                     /locus_tag="VH419_17975"
     CDS             4167..5765
                     /locus_tag="VH419_17975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 5,6-aminomutase subunit alpha"
                     /protein_id="HEY7045565.1"
                     /translation="MTGPSTRSRPHLDLDPQMVKEARRLARKAGAPIVRLAKRHTTVS
                     VERATLRLAGLSGADPDGTPWVNRLLDAVRADVGLEHGVALPVWDSIATQGFPDLLSL
                     AQKAATGDVRFRIPEGKAATGARAAARRQVTAGIRRIDARRRERERLVKRLGDPKQKP
                     WIYLIVATGDIYEDIPQAQAAAREGADIIAVIRSTGQSLLDYVPEGPTREGFAGTYAT
                     QENFRLMRQALDETSRQLGRYVRLTNYASGLCMPEIASLAGLERLDMMLNDSMYGILF
                     RDINPIRTFVDQRFSRQVHARAGIIINTGEDNYLTTADAVEAAHTVTVSQLLNEYFAK
                     EAGLEDWQLGLGHAFEIDPSLPDSFRLELAHALLARTLFPKAPLKWMPPTRHMTGDVF
                     RGYLLDGFFNLAGAMTDQDILLVGMMTEAVVTPWLSDRDLAVQNVKYVVNAAGRLSED
                     FAPRSDGFIATRARQVLAESIELLEQILDHAGTEFGAGLLDAIADGTFGLMKRPADAG
                     KGLEGVAKKARDYYNPATSILEEQ"
     gene            5849..6619
                     /locus_tag="VH419_17980"
     CDS             5849..6619
                     /locus_tag="VH419_17980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OAM dimerization domain-containing protein"
                     /protein_id="HEY7045566.1"
                     /translation="MTPTILRPYGDTTGDGMVQLSFTLPMPHDKRAEGAAAQLANKMG
                     IDPALVVHAKAMGPDFTFFVVYGRSNDLVDVSKVEVVERDYPLLTPKEVNLAVKRALR
                     RRLVVVGGCIGTDAHTVGIDAILNIKGFAGEKGLEYYRELRVVNLGAQVSVPELVARA
                     REERADAVLVSQVVTQRDAHILNTKEMSAAFREAYPEGQRPVLIIGGPRFEERQATEL
                     GVDRVFTRGTTPGEVASYLVHRVIGQRAATSSAATVSA"
     gene            6616..>6978
                     /locus_tag="VH419_17985"
     CDS             6616..>6978
                     /locus_tag="VH419_17985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold domain-containing protein"
                     /protein_id="HEY7045567.1"
                     /translation="MTASAEPDPRVGLTVTHRRYVPYSHAHYAGNLVDGAYSLGLFGD
                     VATEVCIRTDGDEGLFASYSDVQFLAPVRAGDVLEIEAVVTRVGRRSRTIEFTARVTC
                     RGRPESSESAAEVLAEPIV"
BASE COUNT         1060 a         2428 c         2408 g         1082 t
ORIGIN      
        1 cgggtcgtag tactcgtacg tacgggtcaa cgcgtcaggg tcagtacgct cgatcgaggt
       61 ccggtagttc ttcgtccagt acgagatccc gtgctcacgg tcgtacgact cgagctggtg
      121 cacccagcgc ttgccgacga agggcacgtc gcacacgatg cgcggcgtcg cgaagccggg
      181 caggtagccc atgatcccgt gctgaagctc ctgcgcccgg gccaccgaga cccgccagtg
      241 ctcgctgaac gggatcatgt cgcacatgta gaagtagtag ggcatgatct gcgcgccgtc
      301 gagcagcgcg aagcacaggt cgagcagcgt gtgtgcgtcg tcgttgacgc cgcccatcag
      361 cacaccctgg ttgcggacgt cgcggatacc ggtgtccaac atcgcccggg tcgccttcgc
      421 caccatcggc gtcacggagt tggcgtggtt gacgtgggtg tggatcgcga tcgacgcctc
      481 gcgctcccgc gccttcgtgg cgagccgctc catccctgcc cgtacgtcgt cctgcagcca
      541 gtgctgcggc aggcccatca gggccttggt ggccaaccgg atgtcacgga tgttgtcgac
      601 gtcgagcacc tggtcgacga acccctcgag ccgtggccac ggcatgttcg ccacatcacc
      661 gccggacacc acgacgtcgc ggacgccggg ggtcgagcgc aggtactcga tcatggcgtc
      721 cagccggtcc accggcttca ggtggaactt gagcttgtcg atcactggcg tcgagttgcc
      781 gaccaggtcc atccgggtgc agtgaccgca gtactgcggg caggtcggca gcacctcggc
      841 cagcaccttg gtcgggtagc ggtgcgtcag accctcggcg acccacatgt cgtgctcgtg
      901 caggctgtcg cgggtcgcgt gcgggtggct cggccagtcg gtacgccggt cggagaacac
      961 cggcagcatg taacggcgca ccgggtcggc gtacagcagg tcggtcgtcg gcgtcgcgtg
     1021 cggcaccatc gtgttgacca tctgcggcgg catcagcatc gacatcgtgg cgcgctcctg
     1081 ctggtcgcgc tggaggtcgt cgtagaaccg gtcctcgatc aggtcgccca tcacctggcg
     1141 cagctgcttg atgttcttga cgcagtgcgc acgctgccac tggaccgacg cccagtcctc
     1201 ggcggtgacg tcccgccagc ccgggaaccg ggtccagtcg ggctcgacca gctcgacccg
     1261 cttgtacggg tacggctgct ccagaccctc ggccaggaat ggcgtctcgt cacggtccag
     1321 accggtcagg tcggtcatgc tcacctcgcg tgctgatgtg tttgtctcgg gcgtcgatct
     1381 catgtcagag tacgggagaa tatgcggcgt cgccaacgcg acaccgtaag tcttgcggcc
     1441 tggacccgca cacttcggag gttttgtgat cgtgaacgtg gccagcgacc cgatcggcct
     1501 gcatcgggtc gtggcgccgg ccggggtgct gccgcaggcg gcccagcgcc tcgacaccag
     1561 ccccgagatc gggccggacg agatccgcat cgacgtgacc cggctcaacc tcgatgccgc
     1621 gtcgttccgc cagctgttcg acaaacatgg cggcgacgga gcggcggtcc gggcggaggt
     1681 cctggagatc atctcgaccc ggggcaagat gcacaatccg gtcaccgggt cgggtggcat
     1741 gctcaccggc acggtcgccg cggtcgggcc tgagtcgagc gtcggtctca gcgtcggcga
     1801 cgaggtcacg acgctggtct ccctcaccct cacaccgctg gtcatcaccg accggctcgc
     1861 gaactgggac ggacggagcg agcaggtgcc gtgcgagggg tacgcgatcc tgttcggccg
     1921 aaccatcgtg gccaaactgc cgaccgacct cccggcgcgg ctcgcgcttg ccgtgatgga
     1981 cgtgtgcggc gcgcccgccc tcaccgcgcg cgtcgtcggc gggtacgccg ctcgaggctc
     2041 ggcgccgacc gtctgtgtca tcggcggggc cggcaagagc gggtcgttgt cgttggcggc
     2101 ggcgaaggac gcgggagcgg gccggaccat cgccgtcgta cccgtgcagg gggaggtcga
     2161 cctgctcgcg gacaagcaac tggtcgacgt ggtcacgctg gcagacgcgc gcgacccggt
     2221 ggcgctgtcg caggctgtac tggatgccgg gggaccggcg gacgtgaccg tggtctgtgt
     2281 cgacgtaccg ggctgtgagg gcggtgcgat cctgtccacc gcgcagggcg gcacggttgt
     2341 cttcttctcg atggcgacgt cgttcacggc ggccgcgctc ggggcggagg ggttggcggc
     2401 ggacgtgacg atgctcatcg ggaacggcta cctgcccggc cacgccgact acgccgtcga
     2461 cctgctgcgt cgccacagcg gtgtgcgggc gctgttcgag tccaggctcg cgcacgaagc
     2521 ggcaggatag agacaatgac tgcgagctca gagtcagcga tggctgacca cgtcgacgtg
     2581 ccgacaggtg gaaacccggt tccacggcgg gtgctcctgc ggggcggcca catctgctcg
     2641 ccggtgtcgc cgtccgcgac ggccatgttc accgtgaacg gcgccgtcgc ctgggtcggc
     2701 gacgatgcgg cagcctcgtc gtacgccgat acggcagacg aggtcgtcca gctcgacggt
     2761 cggctggtca cccccgggtt cgtcgacgcc cacgtgcacc tggcgcagac cgggttcgcg
     2821 ctcgcgtcgc tggacctctc gacgacccgc acggcggcgg aggcgctgga tctcgtggcg
     2881 gccttcgcgc ggcgcagcga cggcgctgtg ctcttcgcgt acggctggga cgagtcgcgg
     2941 tgggccgacc ggcgtccgtt cacggccggt cagctggacc gcgctgcacc gggccgggtc
     3001 ctgtacgccg cgcgggtcga ctcgcactcg gcggtcgtct cgtcccggct gatcgagcat
     3061 gatccctcga tcaggacgcg ggacgggtgg cggggtgacg gctgggtggc gcgcgacgcc
     3121 caccacgctg ccagggcggt caccgacgca ctgcgaagtc ccagcgaccg ggagcaggcg
     3181 ctgctcgccg cgctgacgca cgcggcgagc cgaggcatca cgtccgtgca cgagctcaac
     3241 gcgccgcaca tcgccccggt ctcggacttc gccgcccttc ggacactcgc cgagcacaca
     3301 ccgcttcccg aggtcgtgcc gtactggggc gagctgctcg gcgagggcct cgacgatgcc
     3361 catgtcaccg ggctgtccgg cttcgccggc gacctctgcg tcgacggggc gatcggttca
     3421 cgcaccgcct gcatgcacgc gccgtacgcc gacgccgaca cctccggcca cctctacctc
     3481 gaccgggacg agatcgccga gcacgtggtg ttctgcaccg atcgtgacgt ccaggccggt
     3541 ttccacgtca tcggcgaccg tgcgatgtcg gagaccgtgg cgggtctcga actcgcggcc
     3601 gacaaggtcg gcaccgacaa gatcgtggcc gcccgccacc ggctcgagca tgtcgagatg
     3661 ccgacgcccg agggggtcgc gaccctggcc aggctcggcg tcgttgccag cgtccagccc
     3721 gccttcgacg ccgcgtgggg cgccgccggc gagctgtacg agcagcgact gggcgctgac
     3781 cgcgcacggc cgatgaaccc gttcgggagc atgcgccggg ccgggatcgt gctcgccttc
     3841 ggttcggaca gcccgatcac gccgatcgac ccgtggtccg ccgtccgagc gtcagtgctg
     3901 caccacgacg aggacgagcg gttgtcgccg caggcggcgt tcgaggcgca cacccggggt
     3961 ggtcatgcgg ctcgccgtga cgacaccggt ggcgtcctcg aaccgggagc gtcggcgtcg
     4021 tacgtcgtct gggacctctc cgacggggcc gtccagacgc cgtccggcgg gtccgcgctg
     4081 cccgacctcg accctcacct tccgctgccc acctgcgtcc agaccgtcgt cgcaggtgtc
     4141 tccgtctact ccgcagagga ctccgcatga ccggcccgtc cactcggtcg agacctcatc
     4201 tcgacctcga cccacagatg gtgaaggagg cgcgccgcct cgcccggaag gccggggcgc
     4261 cgatcgtccg actcgcgaag cggcacacca ccgtctcggt cgaacgcgcg accttgcggc
     4321 ttgccgggct gtccggagcg gacccggacg ggacgccatg ggtcaaccgg ctgctcgacg
     4381 cggtccgtgc cgacgtcggc ctcgagcacg gcgtcgctct gccggtgtgg gactcgatcg
     4441 ccacacaggg gttccccgac ctgctgagcc tcgcccagaa ggccgccacc ggcgacgtgc
     4501 gcttccgcat cccggaaggc aaggcggcaa ccggtgcacg ggcggctgcc cgccgccagg
     4561 tcaccgccgg catccggcgc atcgacgcgc ggcgccgcga gcgtgagcgc ctcgtgaagc
     4621 ggctcggcga cccgaagcag aagccatgga tctacctgat cgtcgcgacc ggcgacatct
     4681 acgaggacat cccgcaggca caggccgcgg cccgcgaggg cgccgacatc atcgctgtga
     4741 tccgttccac cggccagtcg ctgctcgact acgtacctga ggggcctacc cgtgaaggct
     4801 tcgccggcac ctatgccacg caggagaact tccggctgat gcgtcaggct ctcgacgaga
     4861 cgagccgcca actcggtcgc tacgtgagat tgacgaacta cgcctcgggg ctgtgtatgc
     4921 cggagatcgc gtcgctggcc ggcctcgaac gcctcgacat gatgctcaac gactcgatgt
     4981 acgggatcct cttccgcgac atcaacccga tccgcacgtt cgtcgaccaa cggttcagcc
     5041 gccaggtgca cgccagggcc ggcatcatca tcaacaccgg tgaggacaac tacctgacga
     5101 ccgccgacgc cgtcgaggcg gcgcacaccg tcacggtcag ccagctgctc aacgagtact
     5161 tcgccaagga ggccggcctc gaggactggc agctcggcct cggccacgcg ttcgagatcg
     5221 acccgtcgct accggacagc ttccggctcg agctggccca cgccctgctc gctcggacgc
     5281 tcttcccgaa ggctccgctg aagtggatgc cgccgacccg ccacatgacc ggcgacgtct
     5341 tccgcggcta cctgctcgac ggcttcttca acctggccgg cgcgatgacc gaccaggaca
     5401 tcctgctcgt cggcatgatg accgaggcgg tcgtcacgcc gtggctgtcc gaccgcgacc
     5461 tggcggtgca gaacgtcaag tacgtcgtca acgccgccgg ccgcctgagc gaggacttcg
     5521 cgccgaggtc tgacgggttc atcgccaccc gtgcccgaca ggtgctggcc gagtcgatcg
     5581 agctgctcga gcagatcctc gaccacgctg gtaccgagtt cggggcaggc ctgctcgacg
     5641 cgatcgccga cggcacattc ggcctgatga aacggccggc cgacgccggc aaggggctcg
     5701 agggcgtggc gaagaaggcc cgcgactact acaaccccgc gacgagcatc ctggaggagc
     5761 agtagtggct tcgacgagcg caaccagcga tggacggggt cgagcgacgg agtcgccggg
     5821 ctcggctcaa caagccactc gcaccaacgt gaccccgacg atcctgcgac cgtacgggga
     5881 caccaccggc gacggcatgg tccagctgtc cttcacgctg ccgatgccgc acgacaagcg
     5941 ggccgaaggc gccgcagccc agctcgccaa caagatgggc atcgacccgg cgctcgtggt
     6001 gcacgcgaag gcgatggggc ccgacttcac gttcttcgtc gtctacggcc gttcgaacga
     6061 tctcgtcgac gtgagcaagg tggaggtcgt cgaacgcgac tacccgctgc tgacgccgaa
     6121 ggaggtcaac cttgccgtca agcgagcgct gcggcgccgc ctcgtcgtcg tgggcggctg
     6181 tatcggcacc gacgctcaca ccgttggcat cgacgcgatc ctcaacatca agggcttcgc
     6241 gggcgagaag gggttggagt actaccgcga gctgcgggtg gtgaacctcg gtgcgcaggt
     6301 gtcggtgccg gagctcgtgg cgcgggcgag ggaggagcga gccgacgccg ttctcgtctc
     6361 gcaggtggtg acgcagcgcg acgcacacat cctcaacacc aaggagatgt ccgccgcctt
     6421 tcgtgaggcc taccccgagg gtcagcgacc ggtgctgatc atcggcggac cacgcttcga
     6481 agaacgacag gccaccgagc tgggggtcga ccgtgtcttc acccgtggca cgacaccagg
     6541 cgaggtcgcc agttatctcg tgcaccgggt gatcggccag cgggcagcga cgtcgtcggc
     6601 tgcgacggtg agcgcatgac cgcctcggcc gagccggatc cgcgagtcgg actcacggtg
     6661 acgcatcgcc gctacgtgcc gtactcgcac gcgcactacg ccggcaacct cgtcgacggt
     6721 gcctacagcc tcggtctctt cggagacgtc gccaccgagg tgtgcatccg gaccgacggc
     6781 gacgaaggac tcttcgcgtc gtactccgac gtgcagttcc tggctccggt ccgggccggc
     6841 gacgtcctgg agatcgaggc cgttgtcacc cgggtcggca ggcggtcccg gacgatcgag
     6901 ttcaccgccc gggtcacctg ccgtggtcgt cccgagtcga gtgagtcggc agccgaggtg
     6961 ctcgcggagc ccatcgtc
//
